LOCUS       DAOQRO010000001        34288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_678_length_34288_cov_6.087752, whole genome shotgun sequence.
ACCESSION   DAOQRO010000001 DAOQRO010000000
VERSION     DAOQRO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 34288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34288
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_34288_cov_6.087752"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKK20_00005"
     CDS             complement(<1..501)
                     /locus_tag="PKK20_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNU98305.1"
                     /translation="MQRRLMQTVLLAAATMSGGSAHSDEGMWLFNNPPRHILRERYGF
                     EPSNAWLEHLQKASVRFNSGGSGSFVSEDGLVASNHHVGADALQKFGDKDHNYLRDGF
                     HARTLAEEKQCHDLELNVLMSIEDVTGRVNAAVTADLSAEEAFLARRKVMAQIEKESL
                     DTTGLRS"
     gene            complement(717..1856)
                     /gene="ychF"
                     /locus_tag="PKK20_00010"
     CDS             complement(717..1856)
                     /gene="ychF"
                     /locus_tag="PKK20_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNU98306.1"
                     /translation="MLKAGILGLPNVGKSTLFNALTRSRKAPAENYPFCTIEPNLGVV
                     NVPEPRLEALARIAKVPTTIPPAIEFVDIAGLVKGASQGEGLGNKFLSHIREVDALVH
                     VVRCFDDPEIVHVTGSVDPVRDIGIVTTELVLADLEALKRRRERILKEVRGLDKHALA
                     ETHVLDKLEPHLNAGHPALTLNLAREEWDLARPCCLLTTRPTIFAANVKEADLPRLDQ
                     NPYVVQVRDYARRHHACDTVVVCAQLESEAADLTPEEARLYLEELGVKESGLDALIRS
                     TYQLLGLRTFFTFNEKEARAWTIRGGETALRAAGTVHTDFEKGFIKAERVSCADLVRC
                     GSVAHARETGHHIVATLVRVVPRFARVGRDYVVRDGDVLLFKFSV"
     gene            2066..3190
                     /locus_tag="PKK20_00015"
     CDS             2066..3190
                     /locus_tag="PKK20_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU98307.1"
                     /translation="MPGCTKAPSPSSLPPPVVLIAEVTASNVPVVIEYIGQLDSPQNV
                     EVRARVEAFVEKVLFTEGATVNQGDPLYLLDTKPFFERLEAAKGGLAEAWAALNKYEK
                     DVARLRPLAEKRAIPQQDLDNAIASVEVGRANVQSAEARLKSSELDLSYCDVRAPASG
                     RIGAKEVSVGELVGKGQPTLLATISQLDPIWFYGNISEVDFLRGLEVERRFGLRFIDV
                     PLTLILADGSEHAEPGRWVFADRAVDTTTGTLRARAVFPNPGGALRPGMFGRVRMVLR
                     IDRPSILVPQRAVQELQGEHFVWVVDNANTATQRPVKAGVRAGSAWVIDEGLQAGERI
                     IVEGAQKARQGAPVHPMTAEEMAQAAAERQAGAQKPAGKE"
     gene            3194..6358
                     /locus_tag="PKK20_00020"
     CDS             3194..6358
                     /locus_tag="PKK20_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNU98308.1"
                     /translation="MADFFIRRPIVAMVISIVIVLVGLISLTRLPIAEYPAVSPTLIQ
                     ATATYRGAAAEAVMDSVATPIESQVNGVDKMLYLQSVSANDGTMTLNVTFDVGTGVDI
                     MQVNTQNRVSLATPQLPDAVKREGVTVKRSSPDVLLVIGLFSPGRMWDSVFLGNYANI
                     NLVDAIKRVPGVGDVNNFTAQDYAMRIWLRPDKLAILGLTPDDVSSAIREQNAQSPAG
                     VIGAEPAPPGQELQYNVRTLGLLREASEFEEIIVRSNPDGSHVKIKDIGRVELGAQTY
                     ALRARLNQQSCAALGVYLAPGANALATADAIKRLLEDRKAQFPPDMDYEITLDSTLPI
                     KASMNEIVHTLFEALVLVLLVVFVFLQSVRATIIPMLTVPVSLLGVFIAFPLLGFSVN
                     TLTLFGLVLAIGIVVDDAIVVVEAVQHHIERGLTPVAATRQAMREVSGPVVAIALVLC
                     AVFVPVAFMGGVTGRLYQQFAITIAVSVVFSALNALTLSPALSAMLLRKPTPRRGPVG
                     AFFRWFNDTFDRFTHGYTAIVRFLVRKAARSMLLLVAAVGGILILSKLVPGGFIPDED
                     KGYLFLAVELPEGSSLQRTDAVLEKAEAIVMATPGVRSVVAVSGFNLLNSLNTPNAAL
                     MFIGLDDWEKRKTPALQAGAIARQWNRTFFAFPPARIFAFGPPALPGYGNVSGFALQF
                     QDRSGGSVDHLSAGVQQFITEASKRPEIGRLSTTFQPMTPQVRVELDREKARMLGVKV
                     DSVFSTLQAYLSGLYVNDFVRFGRVFKVFVAAEPRFASRPDQVGDLYVRNGDGKMVPL
                     DSLVRIEPMSGPNFAARFNLYPAAEVIGAPAPGYSSAQALRAIEDVARQLPREYGFEW
                     SGLTLQQKRAEGEANVVFGMAIVFVFLLLAAQYESWALPFSVLLATPLVVLGTFTGLL
                     VRRFDLNVYAQIGLIMLIGLAAKNAILIVQFAKMRRDEGREIIEAAVAGSQLRLRPIL
                     MTSFAFILGCVPLMLASGSGAAARSTMGTGVVFGMTVATAVGVFLVPVCYVFVQTLAA
                     RAGKTAPLPAHAASPGGESEGVCG"
     gene            6355..7926
                     /locus_tag="PKK20_00025"
     CDS             6355..7926
                     /locus_tag="PKK20_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNU98309.1"
                     /translation="MSRRWNAGSKRRLAADLTRTRLPDSADAHETVPPARPERTALGQ
                     IRRSVPLALALLAGCAVGPRYQRPALDTPAAYRPARSDGRDLTAPESLGDLDWREVMP
                     DPQLRAYVAEALTNSWDIKAAAARVLQAEAAARAARSQFLPAVGVGGDWATARSSQRG
                     AAAIPRGVGPEAEQFSAYATMASYELDLWGRIRRANQAARARLLATEAARQTVLQTLV
                     AQVATAYLSLLELDCELEIAQNTLEASSDSLALTTAREEGGVASMQDVQQARILVATA
                     QAAIAETHRRIEHKENELSLLLGRNPESPARGEGLLRQRLGPAVPPGLPSSLIERRPD
                     IRAAEQALVAANADIGQAKAAFFPQVTLTGLYGYQAVDLSDLFTAPARTWQFGPAVSA
                     PVFTGGALRANLRLARAKFDEALAQYQRTVQTAFHEVSDALIAYQRTREFRVRQEERT
                     EAHRGATELANTRYEGGVTSYLEVLYNEQELFTSELVLAQARLNELLSVVQLYRALGG
                     GWQAAAPLENTFERN"
     gene            8017..9015
                     /locus_tag="PKK20_00030"
     CDS             8017..9015
                     /locus_tag="PKK20_00030"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006170246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNU98310.1"
                     /translation="MTCAVFDARPYDTDALRHASADLGIDWRFLEFRLTAHTAPAARG
                     AQAVCAFVNDQVDRPCLEALAAEGVKLVALRGTGFNNVDVAAARELGISVTRVPVYSP
                     YAVAEHAVALLLTLNRRIHRAFNRVRELNFSLNGLVGFDLHGKTAGILGTGKIGRVTA
                     QILRGFGMTVLAHDPFKDREWAAREGVDYVDASSLLGLSDVLSLHLPLTPETRHLIRA
                     ETLELVKAGAILINVSRGALIDTTALIEALKTGRLGGVALDVYEEEEGVFFEDLSDQV
                     LQDDELARLLTFPNVLITAHQAFLTREALADIARATVANLEALADGHPFVEGSVLT"
     gene            9207..10643
                     /gene="pyk"
                     /locus_tag="PKK20_00035"
     CDS             9207..10643
                     /gene="pyk"
                     /locus_tag="PKK20_00035"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNU98311.1"
                     /translation="MPFPTHKTKIVATIGPASESPEMLERLIRAGLNVARLNFSHGEF
                     LEHAERIRRIRAAERATGRRVAIMADLPGPKIRIGRIEPEPIQLQAGGSFTLTRADIV
                     GDGQRVSMSLERLPRVVRPGDRLFLNDGLVQLVVERVAGDDVHCHVAVGGELRSRKGL
                     NLPGIDLGMSAFTDRDRACLEFALEHGVDAVSQSFVETPADLEAVRDAARALGKTPFL
                     IAKIERAGALKHLDGILKAADGVMVARGDLGVEVPIEEMALLQKQMIAKASLVGKPVI
                     TATQMLESMVAHRLPTRAEATDVANAVLDGTDCLMLSGESAIGRYPEEAVTMLARIAA
                     FTEAHRPPVRLDDLKALSDPQLPFTASEAIAALVEHALETVPCAAVFVPTRTGTTARM
                     ISRFNPRVWIVALSGDRAVCQGLAFSYGVHPIELADEPEQWPDFARQWLSTQRVPGAL
                     AALVAGPSARNPHANHRLEFLRLDEAST"
     gene            10809..11651
                     /locus_tag="PKK20_00040"
     CDS             10809..11651
                     /locus_tag="PKK20_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNU98312.1"
                     /translation="MQTGLIICQCAHAGLIAPETLGSLTASLRAHGTPFDLVPDLCEL
                     AARRDPRLEAVSRAPRPTVLACHERAVRNLFHMAAAPLDPERALLLDVRTGSAAQALG
                     RLGVPQAAPGSPVEAAPDAPAPAGWIPWFPVIDGARCTNCQQCLGFCLFGVYDLSPEG
                     RVRVASPQSCKTNCPACARICPEVAIIFPKYHESPINGGPVLDEAKEKARIQVDLDAI
                     LGTDVYAKLAARRMEVERRRLLRRDVELADAERQRHLDRESRAESESVERRASSRDRS
                     QGTS"
     gene            11648..12841
                     /locus_tag="PKK20_00045"
     CDS             11648..12841
                     /locus_tag="PKK20_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNU98313.1"
                     /translation="MIPAYFNRLAREVDLGLLARCAWTLGFGGVRSVRAFQKRLRRGR
                     VFPAFLFLSVTNRCNLKCQGCWVTPTTPPRELEPALLDRIIGQAKREQCRFFGILGGE
                     PLLYPRLFEILGRHQDCYFQVFTNGTTLDQEAARAMRRLGNITPLISVEGLEEVSDQR
                     RGGTRVYRRSFQGIECCRRERLLIGVATSICKSNFRDLVSETFVRALIGYGVHYLWYY
                     IYRPMGPDPSAELCLSADDIVALRRFIVDIRPRFPLIVVDAYWDADGRALCPAATGIS
                     HHIGPHGDLEPCPPVQFARENARDSDDTVALVERSAFLGAFRRFAASQTRGCVLLECP
                     DALRAWMAAQGARDTTGRGTALAELAAMQCRPGHHMPGREIPESHWAYRLAKKYWFFG
                     FGAYG"
     gene            12834..14513
                     /locus_tag="PKK20_00050"
     CDS             12834..14513
                     /locus_tag="PKK20_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNU98314.1"
                     /translation="MAEPSPRPAAVSTPDRRLARPAQRVPKDKAMRELLRAAARDHVA
                     RHRLVPPLTLEELRAHTRAVIESLGQDPAYSDFTTVLIGNETWRETLAGIPFDRRVVL
                     LPQCLRTRGDCAAEMDEFGLLCEQCGRCLIGEFQAEAQGLGCVTLVAEGTTVVTRLLE
                     QGRVDAVIGVSCLHVLERAFPHAAMHAIPGIAIPLFRDGCDATGVDADWVREAIRLKS
                     EVVWPGRLDIDALRREVERWFQIDELQSVLGCAGTFTELKAHEWLAQAGKRWRPLLTV
                     CVHNTLAGLADTDSLPDSVRKVAMATECFHKASLLHDDIEDGDEFRYGAPTLARQHGM
                     PIALNLGDYLIGEGYRLLADSGLTPLQRVRAFKIAAEGHRSLCLGQGAELDWMRSASP
                     LTTAQTLDLFRCKTAPAFEVALRLGAVCADADEAIEEPLRRFSAALGIAYQIRDDLDD
                     AFDPQAPGDAAARRPSILVSIACEQASGDARARLQLAFGTEASAATRREIFRDVFLML
                     RTEDKARQLLEHYRNEAQRSLSPLQNAHLKGLLRRLVGRLLGPPPTAPAKA"
     gene            14510..15448
                     /locus_tag="PKK20_00055"
     CDS             14510..15448
                     /locus_tag="PKK20_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNU98315.1"
                     /translation="MSPAAPAPLVPRLRTRCAAALQRALARAASSLDTQARSALSAFV
                     RAQLAADGGFRGRTADGDLYYTMFGLECARALELDLPVEPIRRFVESRLDAPLDFVHL
                     TCLARCIEKLAPGGAPPACGAGLSERLARHACAEGGYHRLPGQRRGSVYESFIGALAI
                     ESLDEPLPNPAGLGAILASLGKASGGFVNSDGWGAASTPVSAAALLLLHELGEPVPPK
                     RVAWLLGQQRAGGGFAAAALAPVSDLLSTATALHALNRIGAIESVAIDSCLEFVTGCW
                     DDSGGFHAHALDPTPDCEYAFYGLLALGNLVAPGEM"
     gene            15463..17295
                     /locus_tag="PKK20_00060"
     CDS             15463..17295
                     /locus_tag="PKK20_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNU98316.1"
                     /translation="MSPDPLDYQSIAAAAHNARRALLERRTPDGHWTGYLSSSALSTA
                     VATVALALTDAKGHDRAVRRGLIWLEEHQDADGGWGDTPSSPANLSTTLLSWAAFDWA
                     SDRMVHRGACERAERWLEKRLGSLAPDQIADRVLDAYGKDRTFSVPILTFCALCGRLG
                     DPDRAWTAVPQLPFELAAMPHRFFKWLRLPVVSYAVPALIAIGLARHEHTGHGVRPLR
                     RLRDALKPRVLGILREIQPAGGGFLEAIPLTGFVVSALAACGLHGHPVAMAGAEFLLR
                     TVRADGSWPIDVNLSTWVTTLSIGALGCGSTPLEGLNAGQRAALVNWLLGQQHQIEHP
                     FTHAAPGGWAWTNLAGGVPDADDTAGALLALHALDRDSPETRAAAARGVEWLLDLQNR
                     DGGIPTFCRGWSNLPFDRSCPDITAHVLAAWATWEPRLETRLRVRLNRAARRALESLL
                     EAQRPDGAWIPLWFGNQHEARLENPVYGTSRVLLAMRRVRGRVGVGVEAAIARGEEWL
                     RRSQREDGGWGGGGESPATVEETALALHALMTGENAGHQTVRRGLAWLASATQGGTVF
                     ASAPIGLYFAALWYAEELYPIIFSCGAFGCAEARRSSTGVFRCC"
     gene            17337..18701
                     /gene="asnS"
                     /locus_tag="PKK20_00065"
     CDS             17337..18701
                     /gene="asnS"
                     /locus_tag="PKK20_00065"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNU98317.1"
                     /translation="MKRTLIKHALAAVEPLEAITVAGWVRTRRDAKGFSFLELNDGSC
                     LGSLQAIVDAGVPGYADMAHCITGASAEVSGRLVPSPAAGQKWELQAARLRLLGKSDP
                     SYPLQKKGHTLEFLRTIGHLRPRSNLFGAIFRTRSRLAYAVHTFFQERDFVYVHTPII
                     TTSDCEGAGEMFRVTTLKGNIDDQGQADFFGRPAYLTVSGQLEAETFACALSNVYTFG
                     PTFRAENSNTARHAAEFWMIEPEMAFCDLEGDMDLAEEFVKALARHALEECAEDLALF
                     GKFVDKGLHDRLQFVVERPFQRVSYAEAVDILRASGKTFEYPVEYGLNLQSEHERYLT
                     EQHFHSPVTVFNYPREIKPFYMRLNDDGKTVTAMDVLVPGIGEVIGGSQREERLDALL
                     ENLSYHKLRPEDYWWYVDLRKYGTVPHAGFGLGFERLLMFLTGVGNIRDVVPFARTPG
                     SAEF"
     gene            19141..19869
                     /locus_tag="PKK20_00070"
     CDS             19141..19869
                     /locus_tag="PKK20_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNU98318.1"
                     /translation="MTGLDERFDGIARLLGRDALARLGRAHVCVVGLGGVGSWAVEAL
                     ARSGVGRMTLVDFDVVSVGNINRQIHALEPTLDQPKAQVLADRVRLIHPRLRLEMIPA
                     RFGEETAARILETPYDCLLDAIDGAANKSRLIAGCRARHIPIVTSGGAGGRRDPTRVR
                     IDDLAFSSHDRLLAQVRAILRQDYAFPRGHAPFGVPCVHSTERPVPAQTPDPARPIFG
                     TAVFVTGTFGFALASLAVKRILGP"
     gene            20000..21097
                     /locus_tag="PKK20_00075"
     CDS             20000..21097
                     /locus_tag="PKK20_00075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HNU98319.1"
                     /translation="MPLAKVLIKETVVTILTGSQRFKHLLADPVKTAAAFRQSGDPYG
                     LGLKGERPEKILVINCGSSSLKYAFFDTASEAEPARGLIERIGGQGMRLVQQGPRGEV
                     ARELPRGGYEEAFQAMLVELTAKETGVLREPSEITVVGHRVVHGGERFSEGVVIDDEV
                     LREIETLNPLAPLHNPVNLAGIQAARRIFPAVPHVAVFDSGFHHTLASYAYLYGLPYE
                     YYEKMGVRRYGFHGSSHAYVCLRAAQHLQRRVNELEIARETLRALNRSYLTRVIEAHR
                     NDPIPIEVSAHHIHLAQEHVEALFGQGHRLTRQSDLSQPGQFACREQLAIVGPKGRVE
                     RVRVLGPMRKATQVEISMTEQFKLDRIQSED"
     gene            complement(21346..22767)
                     /locus_tag="PKK20_00080"
     CDS             complement(21346..22767)
                     /locus_tag="PKK20_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNU98320.1"
                     /translation="MTGFGVVAQATLPMLLDHLRIPLRQITVIDFADREEELRPWMNK
                     GLRFVRERVTPLSLPRLLSTHVGQGGLIVDLAWSIDCFDMLEWAHNNEVLYVNASLES
                     WDPASDMHSKSSLEKSLYARYQKLLPLTARWRDGVTAVIDHGANPGLVSHFVKQGLLD
                     IGAKVARETDLPRGHAARLRRLMKAGDFAALARELGVRVIHCSEWDTQRAARPKRPDE
                     FVATWSVEGMWEESISPSEIGWGTHEKWLPPFSTKPENGTANQIILSQMGLNTWVRSW
                     VPDQEIVGMIVTHGESFGLSHALTVLENGRPVYRPTVHYAYMPCNDSIVSLHELRCRN
                     YELHPKQRILTDEIVEGMDVVGALIMGHRYQSWWTGSMLSIGAARRKLPHLNATAVQV
                     GAGVMAAVLWAIQNPRRGVCFPEDLPHSEVLRWARPYLGRLVSQPTDWTPLRRHRVYF
                     DENPQAQPDQSDPWQFKNFLFRP"
     gene            22970..23848
                     /locus_tag="PKK20_00085"
     CDS             22970..23848
                     /locus_tag="PKK20_00085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNU98321.1"
                     /translation="MRHIKSDTPGREEDSAIPAEGDSNALHVEFERVFGRDRPDNAAS
                     SRRLVGLIETPLGPMLAAASDRAICWLEFARILEPRNMVRSSPVWPSPLSLPFRENSK
                     ERGAGGESWEGIGIGFEAKDGDRSEGRLAREFERMRVEMQRRFGVCIVCGTNPVLERL
                     RVEVRDYFRGKGRTFTVPIELRGTEFQERVWEELQRIPYGATASYEAVAKRIGLPTGA
                     RAVARANATNRLCLIVPCHRVIAKNGALSGYSGGVWRKPLLLELERTGRLPQKTAPIT
                     GCQARPPASEPSRERC"
     gene            complement(23793..24827)
                     /locus_tag="PKK20_00090"
     CDS             complement(23793..24827)
                     /locus_tag="PKK20_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98322.1"
                     /translation="MNPDYRQPIRQRFLRLVLLIALPLATACVASGASIRVGMIGLDA
                     HAVPWTQIMHGPNVARPISEMRVVAAVPAFSADVPFSNDNIAKNIAAMRDLGVEICDT
                     IEAMLGRVDAVMLLSIDGRPHLEQARPVFAARKPLFIDKPVAATLADIIRIFRAAESS
                     RTPCFSNSSLRYSPGIAAMAKDAKVGRVLGCDAYSNNQSILPGHPDLYYYGIHGCEIL
                     FTIMGPGCQTVSRVKGETADIAVGTWNDGRVGSFRGILRGQTGFGALVFGEKGIAPSG
                     PFEGYEPLLVEIAKFFRTGKPPMSAQDTIEIYAFMEAADESLRQEGRPVSISSVLEKA
                     RKQADAPGNP"
     gene            complement(24817..26166)
                     /locus_tag="PKK20_00095"
     CDS             complement(24817..26166)
                     /locus_tag="PKK20_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNU98323.1"
                     /translation="MTTVCGTDFSIHAAEAATVAAILTARAGGTLTLVHACDSAGIDT
                     RSPDAVAALEERGRAQLNAEAERLRKLGATVRTRHLSGSPSSVLLDVAAESKARLMVV
                     SSLGHFPPSRWLVGSVAERLAQNSTIPTLVARGDKPFTAWAAGKRALRVLVGYDFSSS
                     ADAALKWVDTLKQTGRCRITVAYLAWAPEESWRLGVRDDWAITANAAEITKYIKRDLM
                     ERCRDILGTTNVQIRIGDGWGPAGPELVALAKAESADLIVVGTNQRRGLERLWLGSVS
                     RAVLHRAPVSVACIPLPAAEDSPAPTIPEVRRVLVPTDFSTLGNQAIPYAYAALRRGG
                     EVCLMHVAPPLARRGLESEQLESQRAARESSLSARLRQLIPPQAKALGLRTHVQVVDH
                     ANPATAICQAAERFGASLICMSSRGRSRVAKALLGSVAQAVMTGSRRPVLILRDYES"
     gene            complement(26438..27469)
                     /locus_tag="PKK20_00100"
     CDS             complement(26438..27469)
                     /locus_tag="PKK20_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98324.1"
                     /translation="MKYPFEASYAEILADPEEFVTAVFSSLASEFLVLPKGDGFVDYP
                     QFEAGYEALKRVTMGFSVLPQKELLQLVTTKPICLIVLRSILGFTPPEWAYLTSQRKG
                     VAVNQSFVRTLDRKVRIQPLRPLRADGVTKQRLVALVETACDLMEEGCPAVNSHSIHR
                     LHKADTGGGLDTIRALAVMGAPYAMLLYERFLGRPFAGHRDSVSELVGDNLECAIEEV
                     LTKHGISYRKTKRAERIPGFDQAPDFIVPDEFNPEVIIEAKITEDDGTARDKVTRIQH
                     LGELSMGGAAGGKQKYQVIACIAGRGFGVRREDMKKMLLATRGKVFTLKTLDQLVEHS
                     HLRKFKTKV"
     gene            complement(27453..29657)
                     /locus_tag="PKK20_00105"
     CDS             complement(27453..29657)
                     /locus_tag="PKK20_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HNU98325.1"
                     /translation="MIERDFDVPFVADLALREKQIQQNYRPVVAVHKWFARRPGTLFR
                     GLLLSEFLDAPLRQTFYRGQSLPDLRILDPFMGGGTTLIEANRVGCAVTGFDINPMAW
                     WIVRQEIGELNLEEYLQAARVLRGALLNDVSRFYKTQCETCGSEDAHVKYFLWVKSLT
                     CQGCGTLVDLFPNYLLAEDARHPANVFVCRACGQLYESKSRKDPGDCPHCRTAFSIEF
                     TARRNRSKCEHCGALNAYPRPASGPPTHRMFAIEYHCEHCRNSHSGRFFKNPSADDLR
                     QYNEAQACFDALRSTFIPEEEIPAGDETDRLHRWGYRFYREMFNARQLLGLELSCRLI
                     RQHRSPQIAEALATNLSDLIRYQNMLCRYDTMALKSLDVFSVHGFPVGLIQCESNLLG
                     VAGRTATVGSGGWLNITEKFARAKAYCQHPFEVRHLGHRKVQVPIDGEWIGDRRHRAG
                     QTQTKQVRLERADATVVSLEGMKFDAIFTDPPYFRNVQYAELMDFCYVWLRRLALADH
                     PEFAPLTTRHANELTGNDNMGRTLEHFTEGMSRVFQKMASALKPGSPLAFTYHHNRLD
                     AYYPVAVAMLDSGLVCSASLPCPAEMGASIHINGTSSSIIDTVFVCRSTGKVPRRWLS
                     ANPAALAQVVREDLRLLAGGGVKATQGDTRCIAFGHLIRLAVWNLRKGWKLTRPARSR
                     KEAVSKWIEDFGGVEAVLRELGADFTSAQATQQWLASEEPQPYRTRVHEVSF"
     gene            complement(29707..30150)
                     /locus_tag="PKK20_00110"
     CDS             complement(29707..30150)
                     /locus_tag="PKK20_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNU98326.1"
                     /translation="MKTVAVLDASVVIGACGWGAESHLCLVYPAKRRFRSAASALIVD
                     EWRTALGHTAGEGARFRRDPWPTLDWLIRMTGLVTPAPLGKQRSRDAKDDPYLAAALA
                     ARAGFIVSRDPDLLSLGGPFGIEIVTPRAFLSRLTNPHQFRSRRV"
     gene            30379..31641
                     /locus_tag="PKK20_00115"
     CDS             30379..31641
                     /locus_tag="PKK20_00115"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HNU98327.1"
                     /translation="MKAQSVERAYALARERYAELGIDTAQAIRRLARIPLSIHCWQGD
                     DVGGFETAGGTLGDGLAVTGNYPGKARTADQLRADFEKALALVPGTHRFNLHACYAEM
                     GARRVERDALSAAQFKNWIAWAKARKIALDFNQTYFSHPKVDGGMTLTHPDKGIRRFW
                     IEHGIRCREIGAAFGKALGKTCLTNLWIPDGMKDTPADRRGPRQRLAESLDAIFARKI
                     NPVYNVDTVEPKLFGIGAESYTPGSHEFYLGYAVSRKVLLTLDAGHYHPTEGIADKIS
                     SALCFLPEIALHVSRGVRWDSDHVVTLTDDLEAIAQEIVWGGYVDRVHIGLDYFDASI
                     NRVAAWVIGMRNMRRALLLAMLAPIAALKRAELERDYTSRLALMEEAKSLPFGAVWDY
                     ACLQADVPAGEAWLAEVKRYEADVLSKR"
     gene            31716..33113
                     /locus_tag="PKK20_00120"
     CDS             31716..33113
                     /locus_tag="PKK20_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98328.1"
                     /translation="MSEETSGGTGGEFEREPVPEKARKGSSAFWGMYASEHTAGTEFM
                     IGPLFVAWGASAFDMVFGLLLGNLLAVLTWRFLTAEIATARRLTLYYQLERICGRNLV
                     VLYNLFNGILFCFLAGAMVTVSATAVGVPLGESVRMPTFEQTWPTGVVWCLLCMAIGT
                     AMTFVAVRGYGFVARVGHLAAPWMLLVFVACGVVMLAKLGSTNLFELIDPPEGAPRRI
                     GFWGIVFFAWFCNAAVHLGMSDLSILRFARKPSYGWASAAGMFLGHYVAWICAALLLV
                     YWVRQFGVDPQKGAMPGPMVFDAVGVAGLLCVAVAGWTTANPTIYRAGLAFQGMFPRM
                     TRTTATIIAGAFCTVAGVFPAVAMKLLDFVGLYGTILAPVGAVIVVDHFLARRLGLAT
                     NYAERHGIRFNVAVLLAWAIPVAIGLAIYRTQHINSFMLPLPCWVACGALYILFTKLM
                     NRRAGLGLSSSPSCR"
     gene            33121..33294
                     /locus_tag="PKK20_00125"
     CDS             33121..33294
                     /locus_tag="PKK20_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98329.1"
                     /translation="MKTLAKILAPLALAATVVPPLLFLFKAMGEGPMKATMLGAAVVW
                     FAFSPHWMKGGSQ"
     gene            33291..>34288
                     /gene="rhaT"
                     /locus_tag="PKK20_00130"
     CDS             33291..>34288
                     /gene="rhaT"
                     /locus_tag="PKK20_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HNU98330.1"
                     /translation="MNANPFLGVVFHWLGGLASGSFYVPYRFVRKWSWEVYWLVGGFF
                     SWIIAPWILASLKTNDLVTVLSSASAGTLGWAYFWGAMWGLGGLTFGLTMRYLGMSLG
                     MGVALGYCAAFGTLMPPIFHGEFVPKILGTVSGQVILVGVGICLLGIAVAGMAGMSKE
                     REMSYEQKRATIKEFNFVKGILMATFSGVMSACFAYGLDAATPIADLSRRHGTPELWT
                     GLPKLCVVLLGGFTSNFIWCAYLNVRNRTGYQYFSPTLREEHVESPAKTVCGPVAPGA
                     SAVPRLSNYFFSALAGTTWYMQFFFYTMGETQMGDYKFSSWTLHMASIIIFSTMWG"
BASE COUNT         5744 a        11131 c        11234 g         6179 t
ORIGIN      
        1 gctgcgcagg cccgtggtgt cgagtgattc cttctcgatt tgggccatca ccttgcggcg
       61 ggcgagaaac gcctcctcgg cgctgaggtc tgctgtgacg gcggcattca cgcgcccggt
      121 cacatcctcg atgctcatca ggacgttgag ttcgaggtcg tggcactgct tctcctcggc
      181 cagggtccgg gcatgaaatc cgtcgcgcag gtaattgtgg tccttgtcgc cgaacttctg
      241 gagggcatcg gcgccgacgt ggtgattcga ggccacaagg ccgtcttcgg acacgaacga
      301 gcccgacccg ccgctgttga accggacgga ggctttctgg agatgttcga gccaggcatt
      361 cgacggctcg aatccgtagc gttcgcgcag gatgtggcgc ggggggttgt tgaacagcca
      421 cattccttcg tcggaatgcg ctgagccgcc gctcatggtc gcggcggcga gaaggacagt
      481 ctgcatgagt ctccgttgca tagtttgttg gattggcggc agccgcgttc tacacagagc
      541 aaaacaaacc gatacagcgc cgggtcaggg gacccggcct acaaatctgt aggccgcgtg
      601 ccctcacgcg gcggaacttc tgacgctggt atagcccgca tcggtccgcc tcttggagtt
      661 gtcaggttcg cggtggtcac aactgcatga gccctccggg attgttcgcg gcttgttcac
      721 acgctgaatt tgaagagcaa cacgtcgccg tcccgcacca catagtcgcg acccacgcgc
      781 gcgaaacggg gcactactcg cacgagggtc gcgactatgt ggtgccccgt ttcgcgcgcg
      841 tgggccaccg acccgcaccg gacaaggtcg gcgcaactca cgcgctcggc tttgatgaag
      901 cccttctcga aatcggtgtg gacggttccg gcggcgcgca gcgcggtctc gccgccgcgg
      961 atggtccagg cccgcgcttc cttctcattg aaggtgaaga aggtgcggag tcccagcaac
     1021 tgataggtgc tgcggatcag ggcatcgagc ccggattctt tgacgcccaa ttcttcgagg
     1081 tagagtctcg cctcctcggg cgtcagatcg gccgcttcgc tctccaactg cgcgcagacg
     1141 accacggtgt cacaggcatg gtggcgtcgg gcgtaatcgc gcacctggac cacgtagggg
     1201 ttctggtcga gacgcggcaa atcggcttcc ttgacattgg cggcgaagat ggtcgggcgt
     1261 gtggtgagga ggcagcaggg gcgggccagg tcccattcct ccctggccag gttgagcgtc
     1321 agggcgggat ggccggcgtt gaggtgcggt tcgagcttgt cgaggacatg ggtttccgcc
     1381 agcgcgtgct tatcgaggcc ccggacctcc ttgaggatgc gctcgcgccg ccgcttgagg
     1441 gcttcgaggt ctgccagcac cagttcggtg gtcacgatcc cgatgtcgcg gaccggatcg
     1501 acactgccgg tgacatgcac gatttcgggg tcgtcgaagc agcgcacgac gtggaccagg
     1561 gcgtcgactt cgcggatgtg gctgaggaat ttgttgccca ggccttcgcc ctgggaagcg
     1621 cccttgacca ggccggcgat gtccacaaac tcgatcgccg gcggaatggt cgtgggcact
     1681 ttggcgatgc gggcgagggc ttcgaggcgc ggctccggca cgttcacgac gcccaggttg
     1741 ggctcgatcg tgcagaacgg gtagttttcc gccggggcct tgcgggaacg cgtcagggcg
     1801 ttgaacagcg tggatttgcc gacgttgggc aaaccgagga ttccggcttt gagcatgggc
     1861 gtcttgtacg gcctcggccc gaaaatgaac atcctaaaaa tcgctgttcg actttttgcg
     1921 aggatgtgac acagtccgcg caacgcctat gcaaccaggt tcgccgtgcc tgacagatcg
     1981 agccaccgcg tcgccatgcc ccggatcgtc ggggcgatgg tgccggggct tttcctggat
     2041 aggtttgctc gcgacggcgc tgtttgtgcc ggggtgcacg aaggcgccct cgccctcctc
     2101 gctgccgccg ccggtggtcc tgatcgccga ggtcaccgcg agcaacgtgc cggtggtgat
     2161 cgagtacatc ggacaactgg attcgcccca aaacgtcgag gtgcgtgcgc gggtcgaggc
     2221 ctttgtcgag aaggttctgt tcaccgaggg tgcgacagtg aaccagggag acccgctcta
     2281 tctgctcgac accaagccgt ttttcgaacg gctcgaggcc gccaagggcg gcctggccga
     2341 ggcctgggcc gccttgaaca agtatgagaa ggacgtggcc cgcctccgac cgctggccga
     2401 gaaacgcgca atcccgcagc aggatctcga caatgcgatt gcctcggtgg aagtcggccg
     2461 ggcgaacgtg caaagcgccg aggcgcggct caagtcgtcg gaactggatc tctcctattg
     2521 cgatgtgcgc gcgccggcct caggtcggat tggggcgaag gaggtttcgg tgggcgagtt
     2581 ggtgggcaag gggcagccga cgctgctggc cacgatctcc caactggatc cgatctggtt
     2641 ttatggcaac atcagcgagg tggacttcct gcgcggattg gaggtcgagc gccggttcgg
     2701 tctccgattc atcgatgtcc ccctcactct gattctggcc gacggctccg aacacgccga
     2761 gccgggcagg tgggtcttcg ccgaccgcgc ggtcgacacc accaccggca cactgcgggc
     2821 gcgcgccgtg ttccccaacc ccggcggcgc gctgcggccc gggatgtttg gccgggtgcg
     2881 catggttctg aggatcgatc ggccgagcat tctggtcccg caacgcgccg tgcaagagct
     2941 gcagggcgag catttcgtct gggtggtgga caacgcgaac accgccacgc agcgcccggt
     3001 gaaggccggg gtgcgcgccg gcagcgcctg ggtgattgac gagggcctgc aagccggcga
     3061 acgcatcatc gtcgagggcg cgcagaaagc gcggcaaggc gcgcctgtgc atcccatgac
     3121 ggccgaggag atggcccagg cagccgcaga acggcaggca ggcgctcaga aaccggccgg
     3181 caaggagtag cgcatggccg actttttcat tcgccgcccg attgtggcga tggtgatttc
     3241 catcgtcatc gtcttggtgg gcctgatctc cctgacgcgt ctgccgatcg cggaatatcc
     3301 ggccgtgagc ccgacgctca tccaggcaac cgcaacttac cgcggcgcgg cggccgaggc
     3361 ggtgatggac tccgtggcga cgccaatcga atcgcaggtc aacggcgtcg acaagatgct
     3421 ctatctccag tcggtcagcg ccaatgacgg cacgatgacg ctcaatgtca cgttcgatgt
     3481 ggggacgggc gtggacatca tgcaagtcaa cacgcagaac cgggtgtcgc tggccacgcc
     3541 gcagctcccc gacgccgtca agcgcgaggg ggtgaccgtc aagcgttcga gcccggacgt
     3601 cctcttggtg atcgggctgt tctctccggg gcggatgtgg gactcggtgt ttctcggcaa
     3661 ctacgccaac atcaatctcg tcgacgccat caagcgcgtg ccgggcgtgg gcgacgtgaa
     3721 caacttcacc gcccaggact acgccatgcg catctggctg cgcccggaca aattggccat
     3781 tctgggcctg acacccgacg acgtcagttc agccatccgc gagcagaatg cgcagtcgcc
     3841 cgccggggtc atcggggccg agcccgcccc gcccggccag gaactccagt acaacgtccg
     3901 caccctcggc ctgctccgcg aggcgtccga gttcgaggag atcatcgtcc gctcgaaccc
     3961 tgacggctcg cacgtgaaga tcaaggacat cggccgggtg gaactcgggg cgcagaccta
     4021 cgcccttcgc gcccgtctga accagcagag ctgcgccgcg ctcggcgtgt acctggctcc
     4081 cggcgccaac gcgctggcga ccgccgatgc gatcaagcgc ctgctcgagg accggaaggc
     4141 gcagtttccg ccggacatgg actatgagat cacgctggac tcgacgctgc cgatcaaggc
     4201 gtcgatgaac gagattgtcc acacgctgtt cgaagcgctg gtgctggtgc tcctggtggt
     4261 gttcgtcttc ctgcagagcg ttcgcgccac gatcataccc atgctgacgg tcccggtctc
     4321 gctgctgggg gtgttcatcg cgttcccgct gctcgggttc agcgtgaaca cactgaccct
     4381 cttcggtctg gtgctggcca tcgggatcgt ggtcgacgac gccattgtcg tggtggaggc
     4441 ggtgcagcat cacatcgagc gcgggctgac gcctgtcgcg gccacgcgcc aagccatgcg
     4501 tgaagtctcc gggccggtcg tggcgatcgc cctggttctc tgcgcggtgt tcgtgccggt
     4561 ggccttcatg ggcggcgtga cgggccgtct ctaccagcag ttcgcgatca ccattgcagt
     4621 gtcggtcgtg ttctcggcct tgaatgccct gaccctcagt ccggcgctga gcgcgatgct
     4681 cctgcgcaag ccgacgcccc ggcgggggcc ggtgggcgcg ttctttcgct ggttcaacga
     4741 cacctttgac cgcttcaccc acggctacac cgccatcgtc cgcttcctgg tccgcaaggc
     4801 cgcgcgctcg atgctgctgc tcgtggccgc cgtcggcggc atcctgattc tctcgaagct
     4861 tgtcccgggc gggttcattc cagacgagga caagggctac ctgtttctgg cggtcgagtt
     4921 gccggaaggc agctcgctgc agcgcaccga cgcggtgctc gagaaggcgg aggcaatcgt
     4981 catggccacg cccggcgtgc ggtccgtggt tgcggtctcg ggcttcaatc tgctcaacag
     5041 ccttaacacc ccgaatgccg ccctgatgtt catcggtctc gacgactggg agaagcgcaa
     5101 gacgcccgcg ctgcaggccg gcgccattgc gcggcaatgg aaccggacat tcttcgcgtt
     5161 tccgccagcg cgcattttcg cgttcggtcc gccggccctt ccggggtacg gcaatgtgtc
     5221 cgggttcgcg cttcagttcc aggaccgatc gggcggatcg gtcgatcatc tgtcagcggg
     5281 cgtgcagcag ttcatcaccg aggcctcgaa gcgccctgag atcggacggt tgtcgacgac
     5341 gttccagccg atgaccccgc aggtgcgcgt cgaactcgat cgcgagaagg cgcgcatgct
     5401 gggtgtgaag gtcgacagcg tgttctcgac cctgcaggcc tacctgagcg ggctctacgt
     5461 gaacgacttt gtccgattcg gacgcgtgtt caaggtattc gtggccgccg aaccccggtt
     5521 tgccagccgc ccggaccagg tcggcgacct ctacgtgcgc aacggcgacg gcaagatggt
     5581 gccgctcgac agcctggtcc gaatcgagcc gatgtccgga ccgaacttcg ccgcccgttt
     5641 caatctgtat ccggccgcgg aagtcatcgg cgcgccggcg ccgggctaca gctccgccca
     5701 ggccctgcgc gccatcgagg atgtcgcgcg ccaactgccg cgcgaatacg gcttcgagtg
     5761 gtccggactc accctgcagc agaagagggc cgaaggcgag gccaacgtcg tcttcggcat
     5821 ggcgatcgtc tttgtgtttc tgctgctggc ggcgcagtac gaaagctggg ccctgccttt
     5881 ctccgtgttg ctggccacgc cgctggtcgt gctcggcacc ttcaccggcc tgctggttcg
     5941 acggtttgac ctcaacgtct atgcccagat cggcctcatc atgctcatcg gcctggccgc
     6001 caaaaacgcc atcctgatcg tccagttcgc caaaatgcgg cgcgatgagg gccgcgaaat
     6061 catcgaggcc gccgtcgcgg gatcgcagct ccgattgcgt ccgatcctca tgacctcgtt
     6121 cgccttcatc ctgggctgcg tgccgttgat gctggcctcg ggttccggcg cggccgcgcg
     6181 cagcacaatg ggcacgggcg tcgtcttcgg catgaccgtg gccaccgcgg ttggggtgtt
     6241 cctcgtgccg gtctgctatg tgttcgtgca gaccctggcc gcgcgcgcgg gcaagaccgc
     6301 cccgctgccg gcgcatgccg cctcgcccgg cggcgaatcg gaaggagtgt gcggatgagc
     6361 cgtcgatgga atgcgggatc gaagcgccgg cttgcagccg acttgacgcg cacgcgcctt
     6421 ccggattccg cggatgcaca cgagacggtt cctcctgccc ggcccgagcg cacggcactc
     6481 ggccagattc ggcggtcagt gcctcttgcg ctcgccctgt tggccggctg cgctgtcgga
     6541 ccgcgctacc aacgccccgc cctggatacg cctgccgcct atcgccccgc gcggtcggac
     6601 gggcgggatt tgacggcgcc cgagtcgctg ggggacctcg actggcggga ggtcatgccc
     6661 gatccgcagc ttcgggccta cgtggccgag gcgctcacca acagttggga tatcaaggcc
     6721 gccgccgccc gtgtcctcca agccgaggcg gccgcgcgcg cggcccggtc ccagttcctg
     6781 cccgcggtgg gcgtgggcgg cgactgggcc accgcccgca gttcgcagcg cggcgccgcc
     6841 gccatcccgc gaggagtggg tccggaggcg gagcagttca gcgcctacgc cacgatggcc
     6901 agctacgaac tggatctctg gggccgcatt cgccgggcca accaggccgc tcgcgcccgg
     6961 ttgctggcca ccgaggcggc gcgccaaacc gttctccaga cgctcgtcgc ccaggtcgct
     7021 acggcgtatc tgagcctgct cgaactcgac tgcgaactcg agatcgcgca gaacacgctc
     7081 gaagcaagca gcgattcgct ggccctcacc acggcgcgcg aggagggcgg agtggcttcc
     7141 atgcaggatg tccagcaggc gcgcatcctc gtcgccaccg cccaggccgc catcgccgaa
     7201 acccatcgcc gcatcgagca caaggagaac gaactgagcc tgctcctcgg acgcaacccg
     7261 gagtctcccg cgcgcggcga gggattgctg cgccagagac tcggccccgc cgtgccgccc
     7321 ggattgccgt cgtccctgat agaacggcga ccggacattc gcgcggcgga acaggccctc
     7381 gtggccgcca acgccgacat cggccaggcc aaggcagcct tcttcccgca ggtgacgctg
     7441 acgggcctct acggctatca ggccgtcgat ctctcggacc tcttcaccgc gcctgcacgg
     7501 acctggcaat ttgggcccgc ggtttcggcg cctgtgttca ccggcggggc gctgcgggcc
     7561 aatctgaggc tcgcgcgcgc gaagttcgac gaggcgctgg cccaatacca gcggactgtg
     7621 cagacggcgt ttcacgaggt ctcggacgcg ctcatcgcct accagcggac gcgcgagttt
     7681 cgtgtgcggc aggaggaacg cacggaggcg catcgcggcg cgacggaact ggccaacacg
     7741 cgctatgaag ggggagtcac cagctatctg gaagtgctct acaacgagca ggaactgttc
     7801 acgtccgaat tggtgctggc ccaggcccgc ctcaacgaac tgctgagcgt cgtccagctc
     7861 taccgggccc tggggggagg ctggcaggcg gcggcgccac tcgagaacac attcgagcgg
     7921 aattgaagtg cgttcttgcc ggtcagagac gaaaccgatt ttctggttcg cagttttctg
     7981 cggaaagcct gaaatggatc gagttgaatg cctgtgatca cctgcgccgt ttttgacgca
     8041 cgcccctacg acaccgacgc gctgcgacac gcctcggctg atctgggcat tgactggcgt
     8101 ttcctcgagt tccggctgac cgcccacacc gcacccgcgg cccggggcgc ccaggccgta
     8161 tgcgccttcg tcaacgacca ggtggatcgc ccgtgtctcg aggccctggc ggccgagggc
     8221 gtcaagctcg tggccctgcg tggcaccggc ttcaacaacg tggatgtcgc cgcggccagg
     8281 gagttgggca tcagtgttac ccgcgtgccg gtctactcgc cctacgccgt ggccgagcac
     8341 gcggtcgccc tgctgctgac gctcaaccgc cggatccatc gcgccttcaa tcgcgtgcgc
     8401 gaactgaact tctccctcaa tggcctggtc ggattcgacc tgcatgggaa gacggcgggc
     8461 atcctcggga cgggcaaaat cggacgcgtc acggcccaaa tcctgcgcgg tttcggcatg
     8521 acggtgctgg cgcatgaccc attcaaggac cgggagtggg ccgcccggga aggtgtcgac
     8581 tacgtcgacg cctcctcgct tctgggcctg agcgatgtcc tctccctgca cctgccgctg
     8641 acgccggaga cccggcacct catccgcgcc gaaacgctcg aactggtcaa ggccggcgcc
     8701 attctgatca acgtcagccg cggcgcactc atcgacacca ccgcgctcat cgaggcgctc
     8761 aagaccgggc ggctgggcgg ggtggcgttg gacgtctacg aggaggagga aggggtgttt
     8821 ttcgaggacc tctcggacca ggtactccag gacgacgaac tggcgcggct gttgacgttt
     8881 ccgaatgtgc tgatcaccgc acaccaggcg ttcctgacgc gcgaggcgct ggcggacatc
     8941 gcgcgcgcaa ccgtcgccaa cctcgaggcg ctggcggacg gacacccgtt tgtggaaggg
     9001 tccgtgctca catagttctt cccggaagga cggaacactg acaacaaccg gatgcaccga
     9061 ccgaacattg ggcttcatcc caggccgcca gtgtgccatc ttgcggccat gtcgagccgc
     9121 gttcggacca gactcgttga gggccgcgtt ggcccggctt cgtcacgagg cgccgtgcga
     9181 ttctgctttt ccatctgact gttgccgtgc cctttcccac tcataaaacg aagatcgtcg
     9241 cgaccatcgg gccggcttcg gaatcgcccg agatgctcga acggctcatc cgcgccgggc
     9301 tgaacgtcgc gcggctcaac ttctctcatg gcgagttcct cgagcacgcg gagcgcatcc
     9361 gccgcatccg cgccgccgaa cgggccacgg gacggcgtgt tgcaatcatg gccgatctgc
     9421 ccggccccaa gatccggatc ggaaggattg agcccgagcc gatccaactg caggccggcg
     9481 gctctttcac actcactcga gccgacattg tcggcgacgg gcaacgggtc tcgatgagcc
     9541 tcgagcggct gccgcgggtt gtcaggccgg gcgaccgcct gttcctcaac gacggactgg
     9601 tccaactggt agtcgagcgg gtcgcggggg acgacgtgca ctgccacgtg gcggtgggcg
     9661 gcgagctccg atcgcggaaa ggcctcaatc tgccggggat cgatctgggc atgagtgcgt
     9721 tcaccgatcg cgaccgcgcc tgcctcgaat tcgcgctcga acacggcgtg gacgccgtga
     9781 gccagtcgtt cgtcgagacg ccggcggacc tcgaagcggt gcgcgacgcg gctcgggccc
     9841 tgggcaagac gccgttcctc atcgcgaaaa tcgagcgcgc cggggcgctc aagcacctgg
     9901 acggcatctt gaaggcggcg gatggtgtga tggtggcgcg cggcgacttg ggagtcgagg
     9961 tgccgatcga ggaaatggcc ctcctccaga agcaaatgat cgccaaggcc agcctcgtcg
    10021 gcaaaccggt catcacggcc acccaaatgc tcgaatcgat ggtcgcgcac cgcctgccca
    10081 cgcgcgccga ggccaccgac gtcgccaatg ccgtcctcga tggaaccgat tgcctgatgc
    10141 tgtcgggcga gtcggccatc ggcaggtatc ccgaggaagc cgtgacgatg ctcgcgcgga
    10201 tcgctgcttt taccgaggcg caccgcccgc ccgtgcggct tgacgacctc aaggcgctgt
    10261 ccgatccgca gctcccattc acagcgtcgg aagccatcgc tgcgctggtc gagcatgcgc
    10321 tcgagacggt gccgtgcgcc gctgtcttcg tgccgacgcg cacgggaacc acggcccgga
    10381 tgatctcccg tttcaatccg cgcgtgtgga tcgtggcgct gagcggcgac cgggcggtct
    10441 gtcaggggct ggccttctcc tatggcgttc atcccatcga gctggccgac gagcccgagc
    10501 aatggcctga cttcgcccga cagtggctga gcacgcagcg ggtccccggc gccctggccg
    10561 cgctggtcgc cggaccgtcg gcacgcaacc cgcacgccaa tcatcgcctc gaattcctcc
    10621 ggctcgacga ggcatcgaca tgacatgtcc gacaactcgg agttgcgccc ttgcagcctt
    10681 tgcggcagca gccggtattt ggacattccg atccggtatc ctatagtgga agtcagccat
    10741 gcgatgcaga atccgaatga ctcgaggtcc cgaagcgtcc ggcttatgtt ccgagtgaaa
    10801 acgccccgat gcagacaggc ctgatcatct gccaatgcgc ccacgccggg ctcatcgcgc
    10861 ccgagaccct gggcagcctg accgcaagtc tccgggctca cgggacgccg ttcgacctgg
    10921 tgccggattt gtgcgagttg gcggcgcgcc gggacccgcg tctcgaggcc gtgtcccgcg
    10981 cgccgcgccc gacggttctg gcctgccatg aacgcgcggt caggaacctg ttccacatgg
    11041 ccgctgcacc gctggacccg gaacgggctc tgctgctcga cgtgcggaca ggatcggcgg
    11101 cccaggcgtt ggggcgcctg ggcgttcccc aagccgcacc cggcagtccc gtggaagccg
    11161 caccggacgc cccggcgccc gcgggctgga ttccgtggtt tccggtgatc gacggggcgc
    11221 gttgcaccaa ttgccagcaa tgcctcgggt tctgcctgtt cggcgtctac gatctctcgc
    11281 ctgaaggccg cgtccgggtg gccagcccgc aatcctgcaa gacaaactgc cctgcgtgcg
    11341 cccgcatctg ccccgaagtg gcgatcatct ttccgaaata ccacgagtcc ccgatcaacg
    11401 gcggtccggt cctggacgaa gcgaaggaaa aggcgcgcat ccaggtggac ctcgatgcga
    11461 ttctgggtac ggacgtctac gccaagctgg ccgcgcgccg catggaagtt gagcgcaggc
    11521 gtcttctgcg gcgggacgtg gaactggccg acgcggagcg ccaacggcac ctggaccgcg
    11581 agagccgggc ggagtcggag agtgtagagc ggagggcatc gtctcgagac cgaagccaag
    11641 gcacatcgtg attcccgcgt atttcaaccg gctggcgcgc gaggtcgacc ttggtctgct
    11701 ggcgcgatgc gcatggacgc tcgggttcgg tggtgtgcgc agcgtgcggg cctttcagaa
    11761 gcgactgcgg cgaggccggg tgtttccggc gttcctgttt ctctccgtga ccaaccggtg
    11821 caacctgaag tgccaaggct gctgggtgac gccgaccacg ccgccgaggg aactcgagcc
    11881 ggcgctcctc gaccgcatca tcggccaggc caaacgcgaa caatgccgtt ttttcggcat
    11941 cctgggcggc gaaccgctgc tttatccgcg gctgttcgag attctcgggc ggcaccagga
    12001 ctgctatttc caggtgttca ccaacggaac gacgctcgat caggaggcgg cgcgcgcgat
    12061 gcgccgcctg ggcaacatca cgccgttgat cagtgtggaa ggcctcgagg aggtgagcga
    12121 ccaacgccgg ggcggcacgc gcgtttaccg ccgcagcttc cagggaatcg agtgctgccg
    12181 ccgcgagcga ctgctcatcg gagtcgccac gagcatctgc aaatcgaact tccgcgactt
    12241 ggtgtccgag accttcgtcc gggcgttgat cgggtacggc gtccattacc tgtggtacta
    12301 catctaccgg cccatgggac ccgacccgtc ggcggaactc tgcctgtcgg ccgacgacat
    12361 cgtggcgctg cggcgcttca tcgtcgacat ccgcccgcgg ttccctctga ttgtcgtcga
    12421 cgcctactgg gacgccgacg gccgcgcgtt gtgtccggcg gccacgggca tcagccatca
    12481 catcgggccg cacggcgatc tcgagccgtg cccccccgtg cagttcgctc gggagaacgc
    12541 gcgcgactcg gatgacacgg tggcgctcgt cgagcggtcg gcttttctgg gtgcctttcg
    12601 gcgtttcgcg gcctcgcaaa cccgcgggtg cgtgttgctc gagtgcccgg acgcgttgcg
    12661 cgcatggatg gcggcccagg gggcccgcga caccacgggc cggggcacgg ctttggcgga
    12721 actggccgcc atgcagtgcc gccccgggca tcacatgccg gggcgagaga ttcccgagag
    12781 tcactgggcc tatcggctgg ccaagaaata ctggtttttt ggatttggcg cttatggctg
    12841 aaccttcccc tcgacccgcg gcggtttcga cgccggacag aaggctggcg cgcccggcgc
    12901 agcgtgtgcc caaggataag gccatgcgcg aactcctgcg ggccgcggcg cgcgaccatg
    12961 tcgcgcgcca ccggctcgtc ccaccactca cgctcgaaga gttgcgcgct catacgcgcg
    13021 ccgtgattga gtcgctcggc caggacccgg cctacagcga tttcaccacc gtcctgatcg
    13081 ggaatgaaac atggcgcgag acgctggcgg gcattccatt cgaccggcgt gttgtcctgc
    13141 tgccccaatg cctgcgcacg cgcggcgact gtgcggcgga gatggacgaa ttcggcctgc
    13201 tgtgcgagca gtgcgggcgc tgtctgattg gcgagttcca ggcggaggcc cagggtctgg
    13261 gctgtgtcac actcgtggcc gaaggcacaa ccgtcgtcac ccggctcctc gaacagggcc
    13321 gggtcgatgc ggtcatcggc gtgagctgcc tgcacgtgct cgaacgcgcc ttcccccacg
    13381 ccgccatgca cgccatcccg ggcatcgcca tcccgttgtt ccgcgacggc tgcgacgcga
    13441 ccggcgtgga tgcggactgg gtgcgcgagg cgatccggct caagtccgaa gtcgtgtggc
    13501 ccgggcggct cgacatcgac gccctgcggc gcgaggtcga gcggtggttc cagatagacg
    13561 aactgcagag cgtcctgggc tgcgccggca cgttcacgga actcaaggcg cacgagtggc
    13621 tggcgcaggc gggcaagcgc tggcgtccgt tgctgaccgt ctgcgtccac aacacactcg
    13681 cgggcctcgc tgataccgac tcgctgcccg actccgtgcg caaagtggcc atggccaccg
    13741 agtgcttcca caaggcttcc ctcttgcacg acgacatcga agacggcgac gagttccggt
    13801 atggcgcccc cacattggcg cgccaacacg gcatgcccat cgcgctgaac ctgggcgatt
    13861 atctgatcgg cgagggctac cgcctgctgg ccgacagcgg gctcacgccg ctccaacggg
    13921 tgcgcgcctt caagatcgcc gccgaaggcc atcgctctct ctgtctggga caaggcgcgg
    13981 aattggactg gatgcgctca gcctcgccgc tgacaaccgc ccagacgctc gatttgttcc
    14041 ggtgcaagac cgcgcccgca ttcgaggtcg cgctgcgttt gggagccgtg tgcgcggacg
    14101 cggacgaggc gatcgaagag ccgctgcggc gcttcagcgc cgccctgggc atcgcctacc
    14161 aaatccggga cgacctcgat gacgctttcg atcctcaggc gcccggcgac gccgcggccc
    14221 ggcgcccgtc gatcctggtc tccatcgcct gcgaacaggc gtctggcgac gcccgcgccc
    14281 gcctgcaact cgcattcggc accgaggcgt ccgccgccac ccgtcgggaa atcttccgcg
    14341 acgtcttcct catgttgcgc accgaggaca aagcccgcca actgctcgaa cactaccgca
    14401 acgaagccca gcgctcgctc agccccctcc aaaacgccca tctcaaaggc ctgctgcgac
    14461 ggctcgtcgg ccgcctcctc ggccctccgc caaccgcgcc cgccaaagca tgagcccggc
    14521 cgcgcccgcc cctctcgtcc cgagactccg cacccggtgc gccgccgcgc tccagcgcgc
    14581 cctggcccgc gccgcctcaa gcctcgacac ccaagcccgc tcggccctga gcgcattcgt
    14641 ccgcgcccag ttggccgcgg atgggggatt tcgcgggcgg accgcggatg gcgacctcta
    14701 ctacacgatg ttcggtctcg agtgcgctcg ggcgctcgaa ctcgatctgc cggtcgaacc
    14761 gattcgccgc ttcgtcgagt cccgactcga tgccccgttg gacttcgtgc acctgacctg
    14821 cctggcgcgc tgcatcgaga aactggcccc cggcggcgcg cctccggcct gcggcgcagg
    14881 cctcagtgaa cggcttgcgc gtcatgcatg cgccgaaggc ggctatcatc gactgcccgg
    14941 ccagcgcaga gggagcgtct acgaaagttt catcggcgcg ctggcgatcg aatcccttga
    15001 cgagccgctt ccgaatccag ccggcctggg tgccatcctg gccagcctgg gcaaagcctc
    15061 cggaggattc gtcaacagtg acggatgggg agccgcgagc acgccggtga gcgcggcggc
    15121 gctgctgttg ctgcatgagt tgggggaacc ggtgccgccc aaaagggtcg cgtggctcct
    15181 gggccaacag cgcgcaggcg gcggtttcgc ggcggcggcg ctggcgcccg tatcggactt
    15241 gctctcgacc gcgaccgcgt tgcacgcctt gaaccggatc ggcgcgatcg agagcgtcgc
    15301 aatcgactct tgcctcgagt ttgtgaccgg atgctgggac gattcgggcg ggttccacgc
    15361 gcatgcgctc gatccaacac cggattgcga atacgctttc tacggtctcc tggcgctcgg
    15421 caacctggtc gccccaggtg aaatgtagtc cgctgcagcg tcatgtcccc agacccccta
    15481 gattaccaga gcatcgccgc ggcggcgcac aacgcgcggc gcgcccttct ggaacgtcgg
    15541 acgccggacg gtcattggac ggggtattta tccagcagcg cgctctccac cgccgtcgcc
    15601 acggtggcgt tggctcttac ggatgcgaag gggcacgacc gcgctgtccg ccgcggcctg
    15661 atttggctcg aggaacatca agatgcggac ggcgggtggg gtgacacccc atcgagcccg
    15721 gccaacctca gcacgacgct gctgagctgg gccgcgttcg attgggcctc ggaccgcatg
    15781 gtccacaggg gcgcttgcga gcgtgcggag cggtggctgg agaagcgtct cggcagcctg
    15841 gccccggatc agatcgccga tcgcgtgctc gatgcatacg gaaaggaccg cacattctcg
    15901 gtgccgatcc tcacgttctg cgcgctgtgc ggtcggttgg gcgatccgga ccgagcctgg
    15961 accgcggttc cccagttgcc gttcgagttg gccgcaatgc cgcatcggtt cttcaagtgg
    16021 ctgcgcctgc ccgtggtcag ctacgcagtg cctgcgctca tcgcgatcgg cctggcccgc
    16081 cacgagcaca ccgggcatgg agtccgcccc ttgcgtcggc tgcgcgacgc gctcaaacca
    16141 cgcgttctcg gaatcctcag ggagattcag cccgccggcg gcggattcct cgaagcgatc
    16201 cccctgaccg gtttcgtcgt ctcggctctg gccgcgtgcg gactgcacgg ccatcccgtg
    16261 gcaatggcgg gcgcggagtt tctgctccgc acggtgcggg ccgacggcag ttggccgatc
    16321 gacgtcaatc tgagcacatg ggtcaccacg ttgagcatcg gcgcgcttgg ctgcggctcg
    16381 acgccactcg aaggactgaa tgcgggacag cgggctgccc tcgtgaactg gttgctgggc
    16441 cagcaacatc agatcgagca tcccttcacc cacgcggcgc ccggcggctg ggcatggacg
    16501 aacctcgccg gcggcgtgcc cgatgctgac gatacggcgg gcgccctgct ggcgctgcac
    16561 gcgctggacc gagactcgcc cgagacgcgc gccgccgccg ctcgcggcgt cgagtggctg
    16621 ctcgatctcc aaaaccgcga cggcggcatc ccgaccttct gccgcggttg gtcgaatctg
    16681 ccgttcgatc ggagttgtcc ggacatcaca gcccatgtgc ttgcagcgtg ggcgacctgg
    16741 gaaccgcgcc tcgagacgcg cttgcgcgtg cgcctgaacc gcgccgcgcg ccgcgcgctc
    16801 gaatccctgc tcgaagccca gcgtccggac ggggcctgga ttccattgtg gttcgggaac
    16861 caacacgagg cgcgcctcga gaatccagtg tatgggacgt cacgcgttct cttggccatg
    16921 cggcgggttc gaggccgagt cggagtcgga gttgaggccg ccatcgcgcg tggcgaggaa
    16981 tggctgcgac ggagccagcg cgaggacgga ggttgggggg gtgggggtga gtccccggcg
    17041 accgtcgagg aaacggcgct cgccttgcac gcgttgatga ccggcgaaaa cgccgggcac
    17101 caaaccgtcc gtcgcggcct ggcgtggttg gcgagcgcga cacagggcgg cacggtcttt
    17161 gcctccgcgc ccatcgggct gtatttcgcg gccttgtggt atgccgagga actctatccg
    17221 atcatcttca gttgcggggc cttcggatgc gccgaggcgc gccgcagctc gacaggcgtc
    17281 ttccggtgct gctagtgtcc gcggccagcc ggattgtacg gctcgattct ttgcctatga
    17341 agcgcacgct catcaaacat gccctcgcgg ccgtcgaacc gctggaagcg atcacggttg
    17401 ccggctgggt gcgaaccagg cgcgacgcga aaggtttctc gttcctcgaa ctcaacgacg
    17461 ggtcctgcct cggcagcctg caggcgatcg tcgacgcggg tgtccccggc tatgccgaca
    17521 tggcccactg catcaccggc gcctcagccg aggtctcggg ccgactcgtg ccctctccgg
    17581 ccgcgggcca gaagtgggaa ctccaggccg cgcgcctgcg tctgctcggc aagtccgatc
    17641 cgtcgtatcc gctccagaag aagggccaca cgctcgaatt cctgcgcacg atcgggcacc
    17701 tgcgcccccg ctcgaatctg ttcggcgcga tcttccggac gcgcagccgg cttgcctacg
    17761 cagtgcacac gttcttccag gaacgcgatt tcgtctatgt ccacacgccg atcatcacca
    17821 ccagcgattg cgagggagcc ggcgagatgt tccgcgtcac cacgctcaag gggaacatcg
    17881 acgaccaggg gcaggccgac ttcttcgggc gtccggccta cctgaccgtg agcgggcaac
    17941 tcgaagccga gacgtttgcc tgcgccctgt cgaacgtcta cacgttcggt ccgaccttcc
    18001 gggccgaaaa ctcgaacaca gcccggcacg cggcggagtt ctggatgatc gagcccgaga
    18061 tggccttttg cgacctcgag ggcgacatgg accttgcgga ggagttcgtc aaggcgctgg
    18121 cccggcacgc gctcgaagag tgcgccgagg acctcgcgct gttcggcaag ttcgtcgaca
    18181 agggcctcca cgaccgcctc caattcgtcg tcgaacgccc cttccagcgc gtgtcctacg
    18241 ccgaggccgt cgacattctc cgcgcgtccg gcaagacgtt cgagtacccg gtcgagtacg
    18301 gactcaatct gcaatcggaa cacgagcggt atttgaccga acagcatttc cattctccgg
    18361 tcaccgtctt caactacccg cgcgagatca aaccgttcta catgcgcctg aacgatgacg
    18421 gaaaaaccgt caccgccatg gacgtgctcg tgcccggcat cggcgaagtg atcggcggct
    18481 cccagcgcga ggaacgcctc gatgccctcc tcgagaacct gagctaccat aagctgcggc
    18541 ccgaggacta ctggtggtat gtggacctgc gcaaatacgg caccgtgccg cacgccggct
    18601 tcggcctcgg cttcgaacgc ctgctgatgt tcctcaccgg cgtcgggaac atccgcgacg
    18661 tcgttccctt cgcccgcacc cccgggtcgg ccgagttcta gcgagccgcg cctcagacca
    18721 catccggttc tccccatgag gcgtccccgt gtctccccgc cccttctctc ccgcgcctcg
    18781 ctcagtagaa tccttgagcc gtttgacgca gcacagaaac ttgacttgtc tgcaacatgt
    18841 ttcaccggtc aaggattcca gcccgcatgt ttcatcgagt tgtgaccacg gcgaagccga
    18901 gcactcccaa acacgtgcta atgcgggctg gaaagggctc tgcctgccca tgaaccccct
    18961 tctccatgaa ccgcctgcgc aagccgttga taattaacaa aaggagtaac ctgaacgtcc
    19021 aacgcgcaac ttttaacgaa ccgtttcgtt gaacgttgag cgttggaagt tgggcgttga
    19081 acgttccccg gttcatgggt ccaatgcgca ttaaaccgtt gaatatcacc cccccttccc
    19141 gtgaccggcc ttgacgaacg cttcgacggc atcgcccgct tgctgggccg cgacgcgctg
    19201 gcccgcctgg gccgcgccca cgtctgtgtc gtcggccttg gcggcgtcgg ctcgtgggcc
    19261 gtcgaggccc tggcacggtc gggcgtcggg cggatgaccc tggtggactt cgacgtcgtg
    19321 agtgtcggca acatcaaccg ccagattcat gcgctcgaac cgaccctcga ccaacctaag
    19381 gcccaggtgc tggcggaccg ggtccggctc atccatccgc gcctgcgcct cgagatgatc
    19441 cccgcccgct tcggcgagga gaccgccgcc cggattcttg aaacgccata cgactgcctg
    19501 ttggacgcca tcgacggcgc cgcaaacaag agccgcctta tcgcgggttg ccgcgcccgg
    19561 catatcccca ttgtcacaag cggcggcgcg ggagggcgcc gcgatccaac ccgcgtgcgg
    19621 atcgacgacc tcgcgttcag cagccatgac cgcctcttgg cccaagtccg ggccatcctt
    19681 cggcaggact acgctttccc tcgagggcat gcacccttcg gcgtgccgtg cgtccattcc
    19741 accgagcggc ctgtgccggc acagacacct gatcccgccc gcccgatatt cggaacggcg
    19801 gtgttcgtga cggggacgtt cggattcgct cttgcgagcc ttgctgtgaa acggattctc
    19861 gggccttgag gtcgcagggc gttggacact atgccttgcg ccggagcaag gagtgcggcg
    19921 acaatgaatg tcgagcactc gtttggcctc gcccgattcc gcggttgaag cgggacgtgg
    19981 ttttggggta tatgatccgg tgcctctggc gaaagtgctc atcaaggaaa ccgtcgtgac
    20041 aatcctcact ggcagccagc ggttcaagca cctgttggct gatccggtca agaccgcggc
    20101 ggcgtttcgc cagagtgggg acccttacgg cctgggcctc aaaggagagc gtccggaaaa
    20161 gattctcgtc atcaactgcg gctcgtcatc gctcaagtac gcgttcttcg acaccgcgag
    20221 cgaagccgag ccggcgcgcg gtctgatcga acggattggg gggcaaggca tgcggctggt
    20281 tcagcaggga ccgcggggtg aggtggcgcg cgagcttccg cggggcgggt acgaagaggc
    20341 cttccaggcg atgctggtcg aactgacggc gaaggaaacg ggcgtgctcc gggaaccgtc
    20401 ggagatcacc gtggtcggcc atcgcgtcgt tcacggcggc gaacgattca gcgaaggggt
    20461 ggtcatcgac gacgaagtgc tgcgtgaaat tgagaccctg aacccgctgg cgccgctgca
    20521 caatccggtg aacctggccg gtattcaggc ggcgcggcgg attttcccgg ccgtgcctca
    20581 tgtggcggtg ttcgacagcg ggtttcatca cacactggca tcctatgcct acttgtatgg
    20641 tctgccctac gagtactacg agaagatggg ggtccgccgt tacggattcc atgggagttc
    20701 acacgcctac gtttgtctcc gcgctgcgca gcatctgcaa cggcgggtga acgaactgga
    20761 gatcgcccgc gagaccttgc gcgcgttgaa tcgctcctac ctcacccgcg tgatcgaggc
    20821 gcaccggaac gacccgatcc ccatcgaggt ttcagcgcat cacatccacc tggcccagga
    20881 gcacgtcgag gcgctcttcg gccagggcca tcggctgacc aggcaaagcg acctttctca
    20941 gcccgggcag tttgcctgcc gggaacagct cgcgattgtc ggaccgaaag ggcgtgtcga
    21001 acgcgtgcgc gtgctgggtc ccatgcgcaa ggccacccag gtcgagattt cgatgaccga
    21061 gcaattcaaa ctggatcgaa ttcagagcga ggactgaggg cgacttccat gaaccgaacc
    21121 ttggaggggg cgtcgccgct ccgcaccggt tcatggttcc cgtgcgcatt caaccgttga
    21181 atgtcaatgc ttgtcatgaa cctcgggggg tgcgcctgcc tgccctggtg gccctcgtcg
    21241 gaaccgggga aaggctgttc ccgctcctcc ggttcactgg gcgctgagca ggtcgggatc
    21301 gaccaggacg cttttcatgg cgcagccggc gcaacgccgg ccggctcagg gacggaacag
    21361 gaagttcttg aattgccagg gatcgctctg atcgggctgc gcctgcgggt tctcgtcgaa
    21421 gtagacccgg tgccgccgca acggggtcca gtcggtgggc tgcgagacca gtcgacccag
    21481 gtaggggcgc gcccagcgga ggacctccga gtgcgggagg tcctcgggga agcagacgcc
    21541 gcgccgcgga ttctgaatgg cccacaagac ggcggccatg actccggcgc ccacctgcac
    21601 ggccgtggcg ttcaagtgcg gcagcttgcg ccgtgccgcg ccgatgctca gcatgctccc
    21661 ggtccaccag gactgatagc gatgccccat gatcagtgcg ccgacgacgt ccatgccctc
    21721 gacgatttcg tcggtgagga tgcgctgttt gggatgcagc tcatagttgc ggcatcgcag
    21781 ttcgtgcagc gagacgattg agtcgttgca cggcatgtag gcgtagtgaa ccgtcgggcg
    21841 gtagacgggc cggccgtttt cgaggacggt caaggcgtgg gagaggccga acgactcgcc
    21901 atgtgtgacg atcatgccca cgatttcctg gtcgggcacc caggagcgca cccaggtgtt
    21961 gagccccatc tgcgagagga tgatctggtt ggcggttcca ttctccggct tggtcgagaa
    22021 cggcggcagc catttctcgt gcgtgcccca gccgatctcg ctgggagaaa tcgactcctc
    22081 ccacatgcct tcgacgctcc aggtggccac gaactcgtcc ggacgcttgg gacgcgcggc
    22141 acgctgggtg tcccattcgc tgcaatgaat gacccgcaca cccagctcgc gcgccagcgc
    22201 cgcgaagtcg cccgctttca tcaaccgcct cagccgcgcg gcgtgacctc gagggagatc
    22261 ggtttcccgc gcgaccttgg ccccgatgtc gagcaggccc tgcttgacga agtgcgagac
    22321 caggcccgga ttggcgccgt ggtcgatcac cgccgtcacg ccgtcacgcc accgcgccgt
    22381 cagcggcagg agtttctggt agcgggcgta gagcgatttc tcgagggacg acttgctgtg
    22441 catgtccgac gctgggtccc acgattcgag cgacgcgttg acgtaaagca cctcgttgtt
    22501 gtgcgcccac tcgagcatgt cgaaacaatc gatgctccag gccagatcga cgatcaggcc
    22561 gccctgcccc acgtgtgtgg acaacagccg cgggaggctc agcggcgtga cgcgttcgcg
    22621 cacgaagcgc agccccttgt tcatccaggg acgcagctcc tcctcgcggt ccgcaaagtc
    22681 gatcacggtg atctgccgca acggaatgcg cagatgatcg agcaacatcg gcagcgtggc
    22741 ctgggccacc acgccgaagc cagtcatgag aactctcgat tcgaaatcag tcatggcatc
    22801 tctccgtccg cccctgcgtt cgcagcattg tgacagacaa tctcttgagc gtcaaacaat
    22861 cgatcagaaa cctgtcattt tatcgcaaca aatcgatgaa gtgtgcgatg aagtccaatt
    22921 cgacgtgtcc agcgttgttt gccggctgag ggggcgatag agtgcggata tgcgccacat
    22981 caaatccgac accccagggc gggaagaaga cagcgccatt cccgcggagg gcgattccaa
    23041 cgcattgcac gtcgagttcg aacgggtatt tggacgggac cgtccggaca acgccgcgag
    23101 ttcccgtcgc ttggttgggc tcatcgagac tcccctggga ccgatgctgg ccgcggcgag
    23161 cgatcgggct atctgctggc tcgaattcgc tcgaatcctc gaaccccgaa acatggtgcg
    23221 atcaagtccg gtttggccct cacccctatc ccttccattt cgcgagaatt cgaaggagcg
    23281 aggcgcggga ggagagagct gggaaggcat tgggattggc tttgaggcta aggatgggga
    23341 tcggtctgag gggcgcctcg ctcgagaatt cgaacggatg cgcgtcgaaa tgcagcgccg
    23401 gttcggtgtt tgcatcgtgt gcgggacaaa tccggtgctc gagcgcctgc gcgtcgaggt
    23461 gcgggattac tttcgaggca agggccggac cttcaccgtg cccatcgagt tgcggggcac
    23521 ggaattccag gagcgtgtct gggaggagtt gcagcggatc ccttacggcg cgacagcgag
    23581 ctacgaggcc gtcgcgaaac gcatcggctt acccacgggc gcgcgggccg tcgcgcgggc
    23641 gaacgctacg aatcgcctct gcctgatcgt gccctgtcac cgcgtgatcg ccaaaaacgg
    23701 cgcgctcagc ggatacagcg ggggcgtctg gcgcaaaccg ctgcttctcg aattggagcg
    23761 gacgggccgg ctgccgcaga agaccgcgcc gatcacgggt tgccaggcgc gtccgcctgc
    23821 ttccgagcct tctcgagaac gctgctgatc gagaccgggc ggccttcctg gcgcaggctc
    23881 tcgtcggcgg cttccatgaa agcgtaaatc tcgattgtat cctgggcgga catgggcggc
    23941 ttgcctgtcc ggaagaactt cgcgatctcg accagaagcg gctcgtagcc ttcaaacggg
    24001 ccggagggcg cgatgccttt ctcgccgaag accagcgcgc cgaagccggt ctgtccccgc
    24061 aggatgccac ggaaagagcc gacccgtccg tcgttccagg tgccgacggc gatgtcggcc
    24121 gtctcgccct tgacacggct gacggtctga cagccgggtc ccatgatcgt gaagaggatc
    24181 tcgcagccgt gaatgccgta gtaatagagg tcggggtgtc cgggcaggat ggactggttg
    24241 ttgctgtacg cgtcgcagcc cagcacgcgg ccgaccttgg cgtccttggc catggcggcg
    24301 atgccggggc tgtaacgcag cgaggagttc gagaagcagg gcgtgcggct cgattcggcc
    24361 gcgcggaaga ttcggatgat gtcggcgagc gtcgccgcga ccggcttgtc gatgaacagc
    24421 ggtttgcggg cggcaaagac cggacgcgcc tgctcgaggt gcgggcgtcc gtcgatgctc
    24481 agcagcatga cggcgtccac gcgcccgagc atcgcctcga tggtgtcgca aatctcgacg
    24541 cccaaatcgc gcatggccgc gatgttcttc gcgatgttgt cgttgctgaa gggcacatcg
    24601 gcgctgaagg cgggcacggc cgccaccacc cgcatctccg agatcgggcg cgccacattg
    24661 ggtccgtgca tgatctgcgt ccacggcacc gcatgggcgt ccaggccgat catgccgacc
    24721 cggatggacg cgccggacgc cacacacgcg gtggcgagag ggagggcgat cagcagaacg
    24781 aggcggagga accgctgtcg tatgggttgg cgatagtcag gattcataat cgcgtaaaat
    24841 aaggacgggg cggcggctgc cggtcatgac cgcctgggcc accgaaccga ggagcgcctt
    24901 ggcgacccga gaccgtccgc gggaactcat gcagatcagg ctggcgccga accgctcggc
    24961 cgcctgacaa atggccgtcg cgggattcgc atggtccacg acctgcacgt gcgtgcgcag
    25021 cccgagcgcc ttcgcctggg gcgggatgag ttggcgcaac ctggccgaca gactggactc
    25081 acgcgccgcc cgttgggatt cgagttgttc ggactcgagg ccgcggcgcg caagcggcgg
    25141 cgccacgtgc atgaggcaga cttccccacc ccgccgaagc gcggcgtacg catacgggat
    25201 cgcctgattc cccagagtcg aaaaatccgt cggcaccagc acacgccgga cctcggggat
    25261 ggtcggcgcg ggcgaatcct cggcggcagg caaagggata cacgccacac tcacgggcgc
    25321 ccggtgcagc acagcccgcg acacggaccc gagccacagg cgctcgagtc cgcggcgctg
    25381 attggtcccg acaacaatca gatcggctga ctcggccttg gccagggcga cgagttccgg
    25441 gccggcgggg ccccagccat cgccgatccg aatctggaca ttggttgtgc ccaggatatc
    25501 ccggcatcgc tccatcagat cacgcttgat gtacttcgtg atttcagccg cgttggccgt
    25561 gatggcccaa tcgtcccgca cacccagccg ccaactctcc tccggcgccc acgccaggta
    25621 agccaccgtg attcggcatc ggcccgtttg cttgagcgtg tcgacccatt tcaacgcggc
    25681 gtccgcgctc gacgaaaaat cgtaccccac cagcacgcgc aacgcgcgtt tgccggcagc
    25741 ccatgccgtg aacggcttgt ctccccgcgc cacgagggtc gggattgtcg aattctgcgc
    25801 cagccgctcg gccacgctgc ccaccagcca gcggcttggc gggaaatggc ccagcgacga
    25861 cacgaccatg aggcgcgcct tcgactcagc cgccacatcc agcagcacgg atgaaggcga
    25921 tccggacaga tgcctggtcc gcaccgttgc gccgagtttg cggagccgct cggcttcggc
    25981 gttcaattgc gcccttccgc gttcttcgag cgccgccacg gcgtccggcg atcgcgtgtc
    26041 gatcccggcg ctgtcgcagg cgtggaccag cgtcagcgtc ccgccggccc gggcggtgag
    26101 gattgcggcc acggtggccg cctccgccgc gtggatcgag aaatccgtgc cacaaactgt
    26161 tgtcatgatc gcctcgtcgg ttgcgcagcc tcgagcatgc gtctctccta ctctcttttc
    26221 cggcgcttgg ccataacgga gtgcgcgcag cggagtgcgc gggttcctgc catccccccg
    26281 gatccccctc gattcgcgtc cattcgcgcg agcctgccgg agcctcggcg caggcaggtc
    26341 cattcgcgga tgccctgtga cgaacgcatt gggcgcatga accggaaacg ttcaacgctc
    26401 aacgaagggc tttgttggat cctggcgcgg gcgtcactca caccttggtc ttgaacttcc
    26461 gcagatgtga atgctcgacc agttgatcga gtgtcttaag cgtaaagacc ttgccccgtg
    26521 tggccagcag catcttcttc atgtcctcac ggcgaacacc aaagccccgc ccggcgatgc
    26581 aagcgatcac ctgatacttc tgtttgcccc cagcggcgcc gcccatgctc agctctccaa
    26641 ggtgctggat gcgcgtgacc ttgtcgcggg ccgtgccatc gtcctctgtg atcttggctt
    26701 cgatgatcac ctccgggtta aactcgtctg gcacaatgaa gtccggggcc tgatcgaatc
    26761 caggaatgcg ctcggcgcgt ttggtcttgc ggtagctgat gccgtgtttc gtgagaacct
    26821 cttcgattgc acattccagg ttgtcgccca ccagttcact cacggaatca cggtggccgg
    26881 caaacgggcg gccaaggaac cgctcgtaga ggagcattgc atacggcgcg cccatcacag
    26941 ccagggcgcg gatggtatcg agtccgcctc cggtgtccgc cttgtgaagg cggtggatgc
    27001 tgtgactgtt cacggcgggg cagccttcct ccatcagatc acacgccgtt tcgaccagag
    27061 caacaaggcg ttgtttcgtt acaccgtctg cacgcagggg tcgcagcggc tggatccgca
    27121 ctttgcggtc cagcgttcgc acgaagctct ggttgaccgc gacgcccttt cgctgactgg
    27181 tcaagtaggc ccactctggc ggtgtgaaac ccaggattga tcgcagaacg atcaggcaga
    27241 ttggcttggt ggtaacaagt tgaagaagtt ccttttgcgg gagaaccgag aaacccatcg
    27301 tgaccctttt cagcgcctca tagccggcct cgaactgcgg gtagtcaaca aatccatctc
    27361 ccttcggcag caccaggaac tccgaggcaa gactggagaa gaccgccgta acgaactcct
    27421 cagggtcggc cagaatctcg gcgtagctgg cttcaaaagg atacttcatg cacgcgcgtc
    27481 cggtagggct gtggctcctc gctggccagc cattgttgag ttgcctgagc gctggtgaag
    27541 tcggcaccga gttctctgag gaccgcttcg actccgccaa agtcctcgat ccacttcgag
    27601 acggcctcct tgcgcgatct ggcgggtcgg gtcaacttcc agcctttccg caggttccag
    27661 accgcgagac gaatcaagtg gccgaacgca atgcagcggg tgtctccttg cgtcgccttg
    27721 actccgccgc ctgccaatag cctcagatct tcgcgaacca cttgagcaag ggccgcggga
    27781 ttagccgaca gccagcgacg gggaactttc ccggtggatc ggcagacgaa cacggtgtca
    27841 atgatggagg aactggtgcc gttgatgtgt atcgaggccc ccatttcggc ggggcacgga
    27901 agagaggcgg aacagaccaa ccccgaatca agcattgcca cggcgaccgg gtaataggca
    27961 tccaagcggt tgtggtggta ggtgaacgca aggggtgacc cgggcttcaa cgcgctcgcc
    28021 atcttctgaa acacacgtga cattccctcg gtaaaatgtt cgagcgtgcg ccccatgttg
    28081 tcattgcccg tcaactcgtt ggcgtggcgg gtggtcagcg gtgcgaattc ggggtgatca
    28141 gcaagggcca gcctgcgcag ccacacgtag cagaagtcca tcaactcggc atattgaacg
    28201 ttcctaaagt aaggcgggtc agtgaagatg gcgtcgaact tcatgccctc gaggctgact
    28261 accgtggcat cagcacgttc cagcctcacc tgtttggttt gggtttgacc cgctcggtgc
    28321 cggcggtcgc cgatccattc gccgtcaatg gggacctgga cctttcgatg acccagatgc
    28381 cggacttcaa aagggtgttg gcagtatgcc ttggccctgg cgaatttctc ggtgatgttc
    28441 agccagcccc cactgcccac ggtggcggtc cttccggcga cgccgaggag attcgattcg
    28501 cactgaatca ggccgacggg gaatccgtgg acagagaaga catccagaga cttcaacgcc
    28561 atcgtgtcgt accggcagag catgttctgg taccggatca ggtcggagag gtttgtggcc
    28621 agcgcctcag cgatctgagg gctccggtgc tggcgaatca aacggcagct cagttccaag
    28681 ccgagcagtt gtcgcgcatt gaacatctcc cgatagaagc gatagcccca gcggtgcagc
    28741 cgatccgtct cgtccccagc cggtatctct tcttcgggga taaaagtcga acgtagcgcg
    28801 tcaaagcacg cctgagcctc attgtactgc cgcaggtcgt cggctgacgg attcttgaaa
    28861 aagcggcctg agtggctgtt ccggcaatgc tcgcaatggt actcgatggc aaacatccgg
    28921 tgggtgggcg gaccagacgc tggacgagga tacgcgttga gcgcaccgca atgctcgcat
    28981 ttagaccggt tccgacgagc cgtgaactcg atggagaacg cggtcctgca atgtgggcag
    29041 tcgcccggat ccttgcgtga cttgctctcg tagagttgcc cgcatgcgcg gcaaacgaag
    29101 acgtttgcag gatgcctggc atcttcggcg agcagataat tggggaacag atcaaccagc
    29161 gttccgcagc cctggcacgt cagagactta acccagagga agtatttgac gtgggcgtct
    29221 tcgctcccgc acgtctcgca ctgggtcttg tagaacctgc tgacgtcgtt cagcaacgca
    29281 ccacgaagca cgcgagcagc ctgaaggtac tcctccaagt tcagttctcc gatctcctgt
    29341 cggacgatcc accaagccat tggattgata tcgaagccgg ttaccgcgca cccgacgcga
    29401 ttcgcctcga tcagggtggt gcctccaccc atgaaaggat cgaggattct gaggtccggg
    29461 agggactggc ccctgtagaa cgtctggcga aggggcgcat cgaggaactc cgacagaagc
    29521 agaccccgaa acaaggtccc cgggcgtcga gcgaaccatt tgtggactgc cacgacgggg
    29581 cggtaattct gctggatttg cttctcacgc aaagccaggt cagccacgaa tggcacatcg
    29641 aagtcgcgct cgatcatgag catggagcct gacacagcag gcggcgtatg caacgtgcaa
    29701 gttggttcag acgcgcctcg agcggaactg atgcggattg gtaagacggc tcaggaaggc
    29761 gcggggcgtg acgatttcaa tgccgaaagg tccaccaagg gacagcaggt ccggatcgcg
    29821 agagacgatg aagccggctc tcgccgccaa cgcggcggcg aggtatggat cgtctttggc
    29881 atcccggctg cgctgcttgc ccaacggcgc cggcgtcacc aggcctgtca tgcgaatgag
    29941 ccaatccagg gtcggccacg gatcgcgccg gaacctggcg ccttcccctg ccgtgtgtcc
    30001 gagcgctgtg cgccactcgt cgacgatcaa ggcactcgcc gcggatcgaa accgacgctt
    30061 ggccggatag accaggcaca agtgcgattc cgctccccaa ccgcacgcgc caatcacaac
    30121 gctggcgtca agaacggcaa ccgtcttcaa cgcttggcct ctcttgcgac ctctgcccga
    30181 agctgggtcc tcgccgttcc ggattgaacc gcacaggcat agcgcggcgg agccgcaaac
    30241 caagggtgga gggctgctgt ttttcgttgg cattggggcg cggcctgctg gcatgatccg
    30301 gccaggcagc cgctggatca ggaatcggaa tccgggatgc cgcgccttgc tgaggcgcgc
    30361 ggggaaccac gaagaatcat gaaagcacag tctgtcgaac gcgcgtacgc actggcccgc
    30421 gaacgctatg ccgaactggg gattgacacc gcgcaggcca tcagacgcct ggcacgcatc
    30481 cccctgtcga ttcattgctg gcagggtgac gacgtgggcg gattcgaaac ggccgggggc
    30541 acgctcggag acggcctggc ggtcacgggg aattacccgg gcaaagcgcg taccgccgat
    30601 caattgcggg cggatttcga gaaagccctg gcgctggtcc ccggcacgca ccgattcaat
    30661 ctgcacgcct gctatgcgga gatgggcgcg cggcgtgtcg agcgcgacgc gctcagcgcc
    30721 gcgcagttca agaactggat cgcctgggcc aaggcgcgca agatcgccct cgacttcaac
    30781 cagacctatt tctcgcatcc gaaggtggac ggcgggatga cgctgacgca tccggacaaa
    30841 gggatccggc ggttctggat cgagcacgga atccggtgtc gcgagatcgg ggcggcgttc
    30901 gggaaagcgc tcggaaagac ctgcctcacc aacctctgga ttccggacgg catgaaggac
    30961 acgccggcgg accggcgtgg gccgcggcaa cggctggcgg aatcgctcga tgccattttc
    31021 gcgcgcaaga tcaacccggt ctacaatgtc gatacggtcg aacccaagct tttcgggatt
    31081 ggggccgaga gctacacgcc cggttcgcac gagttctacc tgggctacgc ggtcagccgc
    31141 aaggtgctcc tcaccctcga tgccggccat taccacccga ccgaggggat tgcagacaag
    31201 atctcgtcgg cgctctgttt ccttcccgag atagccctgc acgtcagccg cggcgtgcgc
    31261 tgggacagcg accacgtcgt cacgctgacc gacgatctcg aggcgatcgc gcaggagatc
    31321 gtctggggcg gctacgtgga ccgggtgcac atcggcctgg actacttcga tgcgagcatc
    31381 aaccgggtgg cggcctgggt gatcggcatg cgcaacatgc gccgggcgtt gttgctcgcc
    31441 atgctcgcgc cgatcgcggc gctcaagcgg gccgagctcg agcgcgatta caccagccgg
    31501 ctcgccctga tggaggaggc caagtcgctg ccgttcggcg cggtatggga ctacgcgtgc
    31561 ttgcaggcgg acgtgccggc gggtgaagcg tggctggccg aggtcaagcg gtacgaagcc
    31621 gacgtgctct cgaaacgctg acatgggaat ctcacgcctg gacaggcata tgcatttccg
    31681 ggtctgagcc tacgcaattc acgagcgcaa ctggcgtgag cgaggagacc tcaggcggga
    31741 ccggcggcga gttcgagcgg gaaccggtgc ccgagaaggc gcgcaagggt tcgagcgcgt
    31801 tctgggggat gtatgccagc gagcacacgg ccggcaccga gttcatgatc ggaccgctgt
    31861 ttgtggcttg gggcgccagc gccttcgaca tggtcttcgg cctcctgttg ggcaatctgt
    31921 tggcggtcct gacgtggcgg ttcctgaccg cggagatcgc caccgccagg cggctgaccc
    31981 tctattacca gctcgagaga atctgcggac gcaatctggt cgtcctctac aacctgttca
    32041 acggcatcct gttctgtttc ctggcaggcg ccatggtcac ggtctcggcc acggccgtgg
    32101 gcgtgccttt gggcgagagc gtgaggatgc cgacattcga gcagacctgg ccaacgggcg
    32161 ttgtgtggtg cctgctctgc atggcgatcg gcacggcgat gacgttcgtc gcggtgcgcg
    32221 gatacgggtt tgtggcgcgc gtggggcatt tggcggcgcc ctggatgttg cttgtctttg
    32281 tggcttgcgg cgtggtgatg ctggccaagc tcgggtcgac caatctcttc gagctgatcg
    32341 acccgcctga gggcgctccg cggcgaatcg gcttctgggg catcgtcttc ttcgcctggt
    32401 tctgcaacgc agctgtgcat ctcgggatgt ccgatctctc gatcctgcgc ttcgcccgca
    32461 agccgtcgta tggctgggcc tcggcggcgg gcatgttcct cggccattac gtcgcctgga
    32521 tttgcgcggc cctcctgctc gtctactggg tgcgccagtt cggcgtcgac ccgcaaaaag
    32581 gggcgatgcc cggtccgatg gtgttcgacg cggtgggtgt ggccggcttg ctctgcgtcg
    32641 ccgtggcggg gtggaccacg gcgaacccga cgatctaccg ggccgggctg gcgtttcagg
    32701 ggatgttccc caggatgacc cggacaaccg ccacgatcat cgccggcgcc ttctgcaccg
    32761 tcgccggggt gttcccggcg gtggcgatga agctcctgga tttcgtgggg ttgtacggca
    32821 cgatcctggc cccagttggg gcggtgattg tcgtggatca cttcctggcc agacgactgg
    32881 gcctggccac caactatgcg gagcggcatg ggattcgttt caacgtcgcc gttctgctgg
    32941 cctgggcgat cccggtggcg atcggcctgg cgatctacag gacgcagcac atcaattcgt
    33001 tcatgctgcc gctcccctgc tgggttgcct gcggcgccct ttacatcctg ttcacgaaac
    33061 tgatgaacag gcgggcggga ttgggccttt cgagcagccc gtcctgtcgt taatcgacga
    33121 atgaaaacgc ttgccaaaat cctggccccg ctggccctcg cggccacggt cgtgccgcct
    33181 ctgctcttcc tgttcaaagc gatgggggaa gggccgatga aggccacgat gctgggagcc
    33241 gctgtcgtct ggttcgcatt ctctcctcac tggatgaaag gcggttccca atgaatgcaa
    33301 atccgttcct gggcgtggtt ttccactggc tgggcggcct ggcgtccggc agcttctacg
    33361 tcccgtaccg cttcgtccgc aaatggtcgt gggaggtcta ctggctggtg ggcggtttct
    33421 tctcgtggat tattgcgccc tggattctgg cgtcgctcaa gaccaacgat ctggtgaccg
    33481 tcttgtcctc ggcgtcggcg gggacacttg gctgggccta tttctggggc gcgatgtggg
    33541 ggttgggcgg gctcacgttt ggactcacga tgcggtacct gggcatgtca ctgggcatgg
    33601 gggtggcgct cgggtattgc gcggcgtttg gcacgctgat gccgccgatc tttcatggcg
    33661 agttcgtgcc caagattctc gggacggttt cggggcaggt cattctggtc ggcgtgggca
    33721 tctgccttct gggcatcgcc gtcgccggca tggcgggcat gtcgaaggaa cgcgagatgt
    33781 cctacgaaca gaagcgcgcg accatcaagg agttcaattt cgtgaagggc attctcatgg
    33841 cgaccttctc gggggtgatg agcgcctgct tcgcctatgg cctggacgcc gccacgccga
    33901 tcgccgacct gtcgcgccgg cacggaacac cggaactctg gaccggtctg cccaaactct
    33961 gcgttgtcct gctgggcggg ttcacttcca acttcatctg gtgcgcttac ctgaacgtgc
    34021 gcaaccggac gggctaccag tacttcagcc cgacgctgcg cgaggagcac gtcgagagcc
    34081 cggcaaagac cgtctgcggc ccggttgcgc ccggcgccag cgccgtcccg aggctgagca
    34141 actacttctt cagcgccctg gcggggacga cctggtacat gcagttcttt ttctacacca
    34201 tgggcgagac gcagatgggc gattacaagt tttcgagttg gaccctgcac atggccagca
    34261 tcatcatctt cagcacgatg tggggttg
//
LOCUS       DAOQRO010000002        33848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_700_length_33848_cov_5.562838, whole genome shotgun sequence.
ACCESSION   DAOQRO010000002 DAOQRO010000000
VERSION     DAOQRO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 33848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33848
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_33848_cov_5.562838"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..184)
                     /locus_tag="PKK20_00135"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="PKK20_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112331.1"
                     /note="catalyzes the transfer of electrons from NADH to
                     ubiquinone; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
     gene            940..1701
                     /locus_tag="PKK20_00140"
     CDS             940..1701
                     /locus_tag="PKK20_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNU98331.1"
                     /translation="MPKTPLAAIVFECDRLLQPDRFQDWDGAQNGLQVENRGGVTRIA
                     AAVDATRVTVRLAIETRADLLLVHHGLFWSARHPWTGRHYELLRLLLDHDLAVYSAHL
                     PLDAHPRLGNNARLCAALGFKRIEPFFECKGRTIGCAARGRIGRAELAERLRGAVGGP
                     VRVVPGGPAICRRIGVVSGGVGAELSLAAAGGVDTFITGEGPHWTYGLAEELGINVLY
                     AGHYATETFGVKALAAHLGRKFRLPWSFVDHPTGL"
     gene            complement(1768..2274)
                     /locus_tag="PKK20_00145"
     CDS             complement(1768..2274)
                     /locus_tag="PKK20_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98332.1"
                     /translation="MRFFRKKPDPIAESERQLNARIEALQGELRLLNERLSAPPTASR
                     PGSPTAQSASFAARIPAGSREPRFEAIDHLGVQGAADRDSTPDHFNQLGVRKYDPMTT
                     LRKWLSQIRGTPTSNPKLVNYLAAGSIHGLRPLRYEKRVARNRFLALLAFFVAILWGL
                     IYIYMKNR"
     gene            complement(2311..3006)
                     /locus_tag="PKK20_00150"
     CDS             complement(2311..3006)
                     /locus_tag="PKK20_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98333.1"
                     /translation="MTSPFEETDFVDRDVEIGRAARMSPAGAPPIAGSPVPTRPPTRE
                     ELEVQVGETQSRIAELKRAQEELERQRSALEEARRRRVEFEVGRTETTGHLTRGIGLL
                     EKAEFEARRDAEQLAKTLSGFREALARIESINEKAWTTETWSNELTRALTSIENARME
                     YNAARLKWTLLDDSAAPKGEASAAASADDPLSGWTRRPLRELCRVGLALTWPLAVVGV
                     IALITFCVLLLRQ"
     gene            3229..4443
                     /locus_tag="PKK20_00155"
     CDS             3229..4443
                     /locus_tag="PKK20_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98334.1"
                     /translation="MKPILRVVRGQPSWRVASADVEAFVTRMGGQIGPVTFDRRGRRV
                     QPFAVAPWAEEEEAAALPPILRALRGDFFCLPFGGNAKPFGKERHPLHGETANASWTL
                     ESLEKKGAASILHLSLSTKARRGRVDKFITLVDGHQAVYARHRISGMKGPMCLGHHAM
                     LRFPDHAGSGTVSTSPFLLGQVLPEPTERPEQRGYSLLKPGAEFASLENVPTITGETA
                     DLSRYPARRGFEDLALIVSDSAAPFAWTAVTFPRERYVWIGLKDPRVLRSTLFWFSNC
                     GRHYPPWSGRHVNVMGLEEVTSYFHYGLAESARKNTLSRRGYATSIRLNPDRPLEVNY
                     IMAVSSIPSGFDRVAAVDRSPRAGEGLLVRSASGKTLTIALDIDFLGGGNAGSEVSNG
                     PSLSEGRSRRRA"
     gene            4458..6653
                     /locus_tag="PKK20_00160"
     CDS             4458..6653
                     /locus_tag="PKK20_00160"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HNU98335.1"
                     /translation="MNQKPRIQRGNAPRFSFGLRFSTASLFLALIVGSTLLAAAAAGA
                     KPSSTWPQDYSVRRDDDSGALVLTTPFYTVRHDLRQGGAICQITLTHGKATNLLMAPI
                     ETRVRTDRDAVFADVHDRAPRVTHRREGTTEIVTVDGFLADEKGRPSDVRTRTVFEYH
                     WGYIKVRKELTSSGGAVRARELTPFFTRLAPNLCEYGYREGTTEEEGAPPFSFGSNRW
                     GKLRKGVPADKPVETPYAPRSMMFADPGVEGLEWFVGSDLSQWETQCTGRRGQGRCHL
                     ARGTEPDGMAVSISPLWMADAPVTLPGRLVFDFYIGLPLLEGRALKPWLHTSFNRNRG
                     DWVSPDQIQRWKDSGIQTVHCHNDGDYYGDGLFWRDGSYPPYPDMDRYDQVLDACRKN
                     GIRVATYFSNKELHSSTREFQERGTEWGRNDRKGNLQHNFFKEKSEFGVQMCLRSGWI
                     DFLKHSIDRVLDKHPLDGVYYDWNVALFCSNPRHEGKEPGAVAAGHWDIEELLDLMEW
                     TRRRVGPDGLVIVHNTTTPMFVTENFADYVVANEWGYGSWSKKAPELKDLPLEWSLVG
                     ARSRGVISYGQIDANADRHLHRLFALYALVSGVTPWPASPETFTLFAPLGQIEDIASH
                     RFADWRNTAVRLEGDRCASAVYSRPGQCYLLVANLDPVPKQVRCAVRLDQLPHALPAP
                     TSATLVASGLSSPTDSDPTVGSALDVGRLTGEGIAIPVPGDSTVLIRLR"
     gene            complement(6803..9628)
                     /locus_tag="PKK20_00165"
     CDS             complement(6803..9628)
                     /locus_tag="PKK20_00165"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNU98336.1"
                     /translation="MSMLSINSARNLRFFTLTCLLCCSLAFALRTPQGQAAQAPDAPA
                     GLAERFVQPPAQTRILKIIHGWPDEPGRQDDLIGRLQNQGFGGVVCNVAFENYLESEA
                     KWAAFTRAVEAARQAGMALWLYDEKGYPSANAGGLVLRDHPEWEARGLLAKDAETGAG
                     PATLELPPGRLFLAAAYPVRDGDVDFDGRVDLSAHVRDGRVSWTAPEGRWRLLGVTEA
                     PLYEGTHADGNLWRKMPYPNLLEAAPTARFLELTHARYAKEMGPDLGRHFVATFTDEP
                     SLMSLFLRRMPYRPLPWSTGLSAEFRKRHGYALEDAIPSLLVGAGPRVERHRHDFWQT
                     VGELVAENFFGQIQDWCRRHGVQSGGHLLAEEGLTAHVALYGDFFACLRRLDAPGIDC
                     LTSLPPEVPWHIARLAGSAAELDRRPFVMCETSDHGQVYRPAGDARAKRVVTEAEIRG
                     TCNRLFTGGVNVITSYYSFTGMDDAALRRLNEWVGRCATLLAGGHQVADVAVVYPIES
                     LWTRFIPAEHWANASPGANRIEHLYRNALESLFASRRDLTVIDSRTLIEARVLEGELA
                     HNALRWQVVVLPGIDTLPEAAWDRLDDFMRQGGTVIALGARPANTESEFPDRRIRAWG
                     ERAFGETGDGPIARTAVGRGTGVFLSAGIEALLPRVLDQIIEPDLAVPNPRAPLRLTH
                     RRVDGHEVYFVINDSPVPWQGAIECRASGAAEQWDPATGQRSVWPGKTATELVLPPYG
                     ATFLRFDGPPLPARKLNPGVSPSMTFKELPAAQAAVARGEFVRETMSADSEATRPGRP
                     GWRVEGTLTRSDVDTFLFVRLPFEDSLDLSAADLLEIESWVPEGQHTPTQLLVILHER
                     GGGDFLASTPRSLSAAGYDRCYLPLDRLQLAGWSSDNDATLDRRRIDEIRVGWGGYVG
                     REGERIEFSLRMPRAGAIGAGTGADVR"
     gene            complement(9721..11226)
                     /locus_tag="PKK20_00170"
     CDS             complement(9721..11226)
                     /locus_tag="PKK20_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNU98337.1"
                     /translation="MGNTVRIELLPLGRRLTVAPGTSLQDALFPQGVEFPCGGRGRCK
                     GCRVRVLEGGLPVTPEDKRVLTSKELAEGWRLACRAHARSDLRIELAQWELSILSDNS
                     TFAFTPHDGLGAAVDLGTTTIVAQLVDRRTGNVLGVRMGLNQQAKHGADIMSRIDFAM
                     SAGGQTTLQTLVREQIGGLLAELMSEAPPGAPLERVVLVGNTVMHHLFCGLSVEPLSH
                     HPFDPPEDALKTFSAPDLGWHGLGNATVCFLPCLGGFVGSDVLAGIVATQVNESDRLV
                     GLVDLGTNGEIVIGNRVRLLCASTAAGPAFEGARISMGMRAATGAIAEVALRDSAMQC
                     RVLGGGAPRGICGSGLVDAVACGLDLGLVRPTGRLASGDRWTLAPPVVLTQTDVRELQ
                     LAKGAIAAGFRLLLAQWGAGLEDLECLHLAGAFGNYINRASARRIGLLPLPPDRVRPA
                     GNTALLGAKLALFRESGDGFEYEALRRKIRHVPLNEDPDFHETYVAEMQFS"
     gene            11746..13413
                     /locus_tag="PKK20_00175"
     CDS             11746..13413
                     /locus_tag="PKK20_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU98338.1"
                     /translation="MRITDVLVRNGLLTAEQMADVLRLQQAEGLRLDRAIVRLEYLSE
                     RELLGALGEHLKLKVADLSQLSVGQEVLRLLPAKLVFRKRVFPIGQADGAIEVATSDA
                     FDMSALDEARLLTGLEVRPVLAAAEEIDKLIKAHYGVGGDTIDEMVAGEAGGVSAESA
                     TSEDLLEMAQEASVIKLVNEIIIEAVNERASDIHIEPYEHELSIRYRIDGVLHEASMP
                     PQIHRFQAAIISRLKILANLNIAERRVPQDGRIEFSVGGRQIDVRVSVIPMLFGEGIV
                     MRLLDKSSVLFTLTQLGMDDATFSRFQGLIRRPHGILLVTGPTGSGKTTTLYAALNAL
                     VNSEIKVLTVEDPVEYHLRGVNQIPVDPQVGMTFERGLRAILRHDPDVLMIGEIRDLE
                     TARAAIAAALTGHLVLSTLHTNDAASAPMRLLDMGVEPYLVSSTLIGAMAQRLVRTIC
                     PKCRTTDPADPRTLPEDLGWRPGDVLHRGAGCEACRGTGFRGRTGLYELLTINEPLAE
                     CILHRRPPPELIRAGREDGLRLLREDGWIKVRQGVTTVDEVVTCTAV"
     gene            13430..14635
                     /locus_tag="PKK20_00180"
     CDS             13430..14635
                     /locus_tag="PKK20_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU98339.1"
                     /translation="MADFQYTAKTAAGETVTGVLSAESEATALRILDERNLFPLALRN
                     GSDAAGRQAARGRVRTRDVGIMYGQLADLIGAGVPMLRALDSLVKSTVSARLAGLLRE
                     VRAAVADGKSLTEALREHPEAFPPLHTAMVQAGERASFLEEVLQSLSGFLERLDELRS
                     KVIGALIYPALLAGVGTLVLVGALLFFVPRFEPLLGDARKPLPTELVLWLSGVLRGYW
                     YLALAAGAGLGALVWGALSSERGRRWYEEWRLRVPVVGTALRLLAITRFCRILGTLLA
                     NGVPLLQALSISRGATGSRLLGDRIADAMESVRGGRPLTEPLRVGNLFPEQILAMIRV
                     AEESNRLDKVLLKIADTVERRTNRQVDQVVRLLEPLILCLVAMAIGILALGLLLPIFT
                     MASNLGRAS"
     gene            14701..15231
                     /locus_tag="PKK20_00185"
     CDS             14701..15231
                     /locus_tag="PKK20_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNU98340.1"
                     /translation="MKTRNLAGRIAPVRAPVGFTLIEVLLVIVIIMMLAAALVTFVLP
                     QQAGAERNTTRLLLTQVQTSLDTYRMNVGHYPTEEEGGLGALLVRPQFENEQLSERWQ
                     GPYIKPGTKLLDAWGNQVVYELQDIARTGQTNGLPYRLFSMGPDGQANTEDDLVLETT
                     ADENLDSRSVLETTPR"
     gene            15242..15964
                     /locus_tag="PKK20_00190"
     CDS             15242..15964
                     /locus_tag="PKK20_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98341.1"
                     /translation="MSKTNCQAGYTLLELLLVVLLVLLFTGAAVMNVAPLAQGARLDE
                     GVGQLETLLRFARAEAAQQGRRHQLQIQAASEAQPASRAAIAVRWEPEPLQDPGVFVQ
                     RQSTAALAQSVTEHVRIEHVRLIEPASPSAHPPAARDAWDMSAPASEPPGAPAFGAVE
                     EWPSITFYPDGSSDSAEILVGSTDINDPRLMLVKWNGLSGSTAREGVNPESLGLVGSR
                     TAEAQPLEPASVAHTILGPSAP"
     gene            15979..16410
                     /locus_tag="PKK20_00195"
     CDS             15979..16410
                     /locus_tag="PKK20_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98342.1"
                     /translation="MQLPGPADQTQPGREPRSGRGAVLLEVLLALGLFVVAAAIVTGG
                     LNAAVERTIRLRAQTHALDLAASVISEIQMGLRAAQAAGPESFEAPFDQWTCEIEVGP
                     YTFGTENAAGLQLVTVIVRGETPPMVQRLTTILVSESGEAP"
     gene            16413..17303
                     /locus_tag="PKK20_00200"
     CDS             16413..17303
                     /locus_tag="PKK20_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98343.1"
                     /translation="MRPLRVRAVPLGAGAFTLLEVLLALSIAIGLLIVVLYYYQQAAL
                     LRDSTLDSATGLAAVRLCMDRLGTELRTASAQPDSLRGSSQEIEFLQCELPESGNVAA
                     VDDDAPVSTPVLLRRIRYAMRASEESSAAGGLIRTETVVTPAASGSGEPEPDSVETDP
                     AETGVVEESSEPPDTNDVWAVETASVEEEGTRITTSFPSAGAPPLTIPELRYLRLRYW
                     DGAQWQDSWSGSHLPRGIEVTLALEPVPSDAASDTLPEEVFRRVIAIPAGLASSAAPA
                     VADDYESGSVNGGMTPEGGR"
     gene            17300..18844
                     /locus_tag="PKK20_00205"
     CDS             17300..18844
                     /locus_tag="PKK20_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98344.1"
                     /translation="MKTHCYPRKDRARPWRSGGFVLLIVLVVVLLSSMLAASVLYAMR
                     SEAAAHTASVQREQAWAAAMSGVARALAVATDPGGDWMGWEDNPAMFQHQFVKQDGDD
                     QWFFTVYSASDSLGAEVRFGLTDEASKLSVRHHDPAWLGKLPGLDAKLAAALAGAVPR
                     SETAPRTNGPRAASWAPDVSGRAGANDLPPDARSDETRLSGAPVSEGQPSEAAPSIPA
                     ASASPVPSIEEWFAAAGLSSQLLYGEDANYNLRLDPNEDDGETRKPLDNADGQLDRGL
                     QQHLTTISYDLNVNSDGQPRINLNDAKADLSKSGLPQATLDYVAALRRAGQALSHVAA
                     LLEAEGEFQDAEGRPQRLRSGIGREELSVVLDQCTTTNAARLEGLINVNTAPRAVLIA
                     IPFVGEALADAILATRPGLTADEARTPAWLYRRGLVTAEEFKQIVPHLTTRSLQFSFR
                     CLGYAVPSGRYRVLSVMVDTAPHPPRVAALRDLTRFGFPVPLAVLENGGAGGVSTTSA
                     GGRLAW"
     gene            18855..20735
                     /gene="pilM"
                     /locus_tag="PKK20_00210"
     CDS             18855..20735
                     /gene="pilM"
                     /locus_tag="PKK20_00210"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNU98345.1"
                     /translation="MALNLDHIWTGLAARLNRPTASRRSRRTSITALDLEGGFLEVVQ
                     AASRSGEPRVVRHAAVPLDRPPDADSETGSVSGPVFAAALARAGIRPGAVVMGVPRAR
                     VMLRTLDLPPAEREAEIAAMVHLRLQRDLPFGLDEAVIDFRTLKHPASLQAEPTAPDR
                     PGPQGAATPVRVLAAVVRRDTVEHYQSLAKAAGVKLAGLGLRSMASARAAQCLDSRTT
                     QGCVALISVHRREVLFDVLFEGALVFSQVGALAAGAGPARGPGPALVEPTAESVLTEV
                     VRSLHSYEGVADHHPIGRCLVTGSTGAEPGLAQALASQLGVQAEVLEPAGPWSEASDN
                     AESPTHALPAIGLALGALEPEGLPIDLLNPKRPPVARDNRRARQLGIVAAILAVLLAA
                     AGLRARWIGARERHRAQLQEQITLGSKNLAAYRHVRNQARTLAEWSETGRNWLDHLTV
                     LSALLPPSRDLYVTALSASARNTLNLAVKVRGGETVDRLLETLRSAGYQVKAPAIMPA
                     MDRFGFRFQANLELGIPTAITNNLDALAVESRPPHSPAASEAAASAPTPQAAAPTPQV
                     ALPAPPAASPAPPAEASHGAPPGAVPAAAPASGAWREQRGSQAGGEPRMRRRRPEGGW
                     RE"
     gene            20728..21732
                     /locus_tag="PKK20_00215"
     CDS             20728..21732
                     /locus_tag="PKK20_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98346.1"
                     /translation="MNKRVRLLAWAALGVAGLLAMGFVLRAAINRPLRTLDGQILALE
                     SRLKSLQKERQAFLKADTEVRAGAARLFASQAAEAEARLGALLTAQIVRVGLREADFT
                     RVPVGRRRLPGAEEVGWSVQGEGSLARLLDLLFLLQSEPRLQRIEGLSLSPANEGDRT
                     RIRFRYATLVFLPAVEIEAAIPEAELSLDAPLRRRYDAITQRDLFRPHSPQERAANPP
                     PAGAPTPESEQPHLRIVSLSSWDGQPEVHLFDARQQRLVVRRPGDELLDGEVAAVDYR
                     PQPVPGKAGLISYSRLIWRIDTNYWAVEPGQTLADRRLLAPEELPATLHPIPTHPVLP
                     "
     gene            21729..30446
                     /locus_tag="PKK20_00220"
     CDS             21729..30446
                     /locus_tag="PKK20_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNU98347.1"
                     /translation="MRLDHCICLVCLALSSLAPLRPLAADGAGADTPVPATNSAPDAV
                     PIDAGAATPASVTPPSGTATNAPPDAAGIRFDFAGMPYSEAIQRFCQMAGKPLIADTV
                     VEGALTFSDPKPYTYGEALDTLNLILATKSVMLIEDDRYLRLVPFKALSQMPLKLIRG
                     LENVGDVRPGELVTVVLDLKNLDAGELSQPISAMLSSAGSVASLSRGRGLIITDRLAN
                     IQRVRQLLDQVDTASPVQRQVKTFALRNVSGTLMSDLLNRTFGIATAPKRTVFNEQRK
                     AYEVLPPDPTDYVTAIFAEASNTLVLFGPSERIDMAGDLIARFESDAGAAASEIKVFY
                     PQMPVEELAEMVRQAIPGVAGRGDRGRESDTRAKVITDRTSNRLIVTAPAIGQLDAIT
                     ALIRRLDPSTQETPAEKAPDRSTPQRELRVVDLKSGSAQALTPMLRDALTDLTRDTPG
                     PNPMSQVRIQAAPMGNRILLTGAPAALDDVLRLIEQLDTVSAPAEVTRVFKLRQANAG
                     RVAGLIMGAMGGYDWRRRGGGGAVLAAADDRSNSLIVAASEGQMKMIEQVIEQLEAVG
                     AVGGRQLKILELTNNSAATVAGTLARLFAPQVNSYDPTQRVALAPAPNDRALVLEADE
                     SMMARLEEAVRTLDTAAAAGGIETRVVEVKSGSAQALAPMLNEALYQFGRDGRGASPF
                     GRVQVQSSPVGNRLLLAGTAEALDRVETLVAQLDSVPTPQSTRVFKLKNADARQVANM
                     VLSTMGGYESRRRGGGAGYVAAAAEERSNCVVVSAPDSQLELVEDIINRLDEAGLAGG
                     RKLEILPIDRNSAASIAAMVTQMFYPQMRSSDPDTRIALTAAPDDRALVIEAGESMID
                     RLRETVQALDVPPTLGGIEVRTYALPEGRSVELAEALNRIFADPSESRRRWDQPMSAA
                     PPAPRFEIDSGSELLMVAATADQFERIEKVLDDLRAAAAAASQIRTFRLQHGDPAQVV
                     EVLQSMLLGDASQRWRSSRGWRGGTGSGEVRVAAAPALNAVVVQASPEKLALATQLIR
                     TLDSPRSEDAASIQTVRVEKAQPEAVAAAVTQTLATQSPPNTAPRVSVTAVPGGRSLL
                     LSGPEAEVQRIAALIRELDQDSPSDQIETRVFQLEHGSARELSRVLTQLIEGLSRARP
                     RFGNRFQRDSTVTIAADERTNSLLVSGSADSFKLVEQLLVTLDQAPQHSDRQVQFYWL
                     KNARAFDVALKAEALFAERPRAERPLIEPDSFSNSLTIVAKPADLLEIESVVLKLDEA
                     ARDTSLQVRMIPLTDVPVERMAAMLTNLYSQLNEGQVRLVDRLPSPTPRPAPSLSPVT
                     NAPAATPTRPDAPAAPDAPPSPAAPPEVVIAVDKAANALIASGPAYELDHISSLVYQL
                     SASIASPEFEIRQFRLKEADPVVVARVLNELFKPVPTQAQGQAGQPGGATPRRGADGR
                     REAESQGGGRVDNQGRSQGQPQVVAPKVAVVAESRTHSVIVRARPADFALLESIIEQL
                     DEGGLTAELAHRLVPLEHVTPERVLPLLRQMLTQLTSARPGEPAAIAGDPRSRGIFLV
                     GRQSVLDRIEALIHELDTSSEFAEIELRTLDLKHAQAPAVAGLMKSVLRPDASSELSA
                     EARQLQEQISRLKLLDAQGRPIGLDLTQPIKIVADPAQPNAEGGNRLVIGSTPGNLDA
                     LAAVVALLDTPSAAGAESLRVFPLTHADASSILQVLNDLSTRAAGAGRPRPEDRANVS
                     VDERTNTLLAAGAAKALERIAELVEQLDRELPDDLRGIRILPLTHADATTIASSLQRL
                     LDERARPRGGGRRMGPSSRVAVIPDARSNSLLISGGPDGFDLVQTLVAQLDQPGTSLV
                     GQVRLIPLEHSDAGTLATTLSDLFNRRYQAARTPDMQRNRPVILADPRSNSLLVAAGV
                     DDNQAIDALLVKLDRPPENPSVELSVLGLEYNDSARVAVTIRSVFAARLRSMTPVGQQ
                     PAPSDRVEVEADTLSNALVISASKPNLELVRELVAKLDAEPVATGGLIETFTLEHADV
                     QRVSTLLRSLLDQGLYRPGLAVEGGRRSRRDALALATDPRSNTLIVSASPENLLIVKE
                     LIRQLDTADFTGASDIRLFALKRARASHLATVLEQFFRARRASETAVGAAQRSAPVTV
                     TADDRTNTLLITGGKESFDAVERMIEQLDTDTAMARTNFRVYELRHTTATKLQATLQR
                     LFTNRPPRIQGQPPEPVTVIADGWANALIIGASDDDLAMVASLIEKLDTEQPAGGAQV
                     QVFQMAKADARRVQQTLQSLFRGSAGAGAAPGAALVPVVLDVDERLNAIIVSAGETDL
                     KRIADLVKKLDTDQVARVAEIRIFPLQNGRAVEMALVLNNVLNNNPRALTEASPNRQS
                     LLQFITKTQEGERLITSALKEGILIVPDSRANSLVVSAPLDYMALLSEVISHLDATAP
                     TMAQIKVFHLKNADARQMAQVLTSLFRLQRVGAAGAGQQSVQYVMPATTDATGPEGEA
                     PAEAIGAVVGSAQEDALSVTVDFRTNSLLVGGTEHYVALATQIIATLDSTPAQERTAK
                     VYRLKNARSQAVEASLRTFLQQDLQRIMSILGPSGVGTAQNILDREVSIVSETNSNSL
                     LISASPRYFKEVQGLVEELDQPQPQVLIQVLLAEVTLDSTTQLGVEWDYTSDTDPVVS
                     TGTDFGQRELLQDYGGYWSSVTGSNYRFLVRALQNQGRLEVLSRPQILTADNQEATIN
                     IGQSVPVVQSTQVTPQGGTVSAIEYRDVGVMLTVTPRISPDGYVKMDVAPSISDLSSS
                     KVTISKGEDLPIINQRTATTTVTVKSGQSVLIGGLIGTIDDERTKKVPLLGDIPGLGV
                     LFRSKSKGQTRKELLVVLTPQILLKGEGEGRSFDAQTFTEEELRGSTIQGEFQRDPLQ
                     RRVLDPLLPPKTLESQTPQ"
     gene            30507..31031
                     /locus_tag="PKK20_00225"
     CDS             30507..31031
                     /locus_tag="PKK20_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98348.1"
                     /translation="MISIQFGIHNGLRRAAGACAACLLAVALCGCVTNSKTPRAASSS
                     AIDELNLLGLPVAVNLDRRPGADGVVVKLFATSRSSPRAMPLRSGTLELIAYEGTPSP
                     AALPMPFHRWTFTPAELAHNEFTTVLGTGYRLVLSWMPRSLPSDRVTIIARYHPHKAE
                     PVTSAPSSITASAY"
     gene            31169..31576
                     /gene="rnk"
                     /locus_tag="PKK20_00230"
     CDS             31169..31576
                     /gene="rnk"
                     /locus_tag="PKK20_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNU98349.1"
                     /translation="MSDDRTIYVTTADMARLQALLARQKNAGEALEKLAAELDRARVV
                     APEDIPQDIITMNSTAQLRELLTDDVMTYTLVFPDRADYEAGRISVLAPIGTAMLGQR
                     EGDEFEWEVPAGPVRLRVEKVIYQPEAAGRSDP"
     gene            31811..32218
                     /locus_tag="PKK20_00235"
     CDS             31811..32218
                     /locus_tag="PKK20_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HNU98350.1"
                     /translation="MHSNLDNGPVLTKTRELCETIINQTDFLAMRRDVETFLADDDAR
                     SRYHSLANKGQDLQYKQQQGAQLTREEIEAFEQERETIMQNGVIRGFLEAQQQMQRVH
                     ETVGRYVMKAMELGRVPEEDDFAGCGQGCSCGH"
     gene            complement(32547..33074)
                     /locus_tag="PKK20_00240"
     CDS             complement(32547..33074)
                     /locus_tag="PKK20_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98351.1"
                     /translation="MKWTPRLALVCVVGGWLCPETLPAGWIDILPGRSWTAQFYGARR
                     YVKPIAPQTQLATPPATLSPTPPPLPGTTPPARPQTNTVDAEILKKQTDQKAETLKRT
                     IEFQKKRAEQGSAVAQFDLGKRYLTGDGLEKNLQEARKWFEAAAKQGHAGAAARIEEV
                     GKLEAAATKKAEPSP"
     gene            33351..>33848
                     /locus_tag="PKK20_00245"
     CDS             33351..>33848
                     /locus_tag="PKK20_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98352.1"
                     /translation="MRIVALVCLASSLWRNGVQAGGEDGWLTGVDTWGGRAALPAVVN
                     PAVASPLQSVLSLRGEWEFTTRPVAPLRHPGWNAFYAQPWPDARTIQVPGCWEAQGVG
                     APGMGDSWDCKWDHCAKPLRAVYRGDAWYRKTVAIPEAWRGQRVWLKVGGVRSQGWFW
                     VNRTPV"
BASE COUNT         5721 a        11114 c        11220 g         5793 t
ORIGIN      
        1 tcgccgcgat tccggcgaca tgggcgccct ggaagcgcgc ttgagccagt tccctgacgc
       61 tgggctgacg cgtcccgtcc gatccggcga ttgtgccggc gcccaagggc gaaccgcctt
      121 tcatctcgcc aacatcgctc aggtcgggac atgaatacgg cagtccggca tagatcatgc
      181 cgtagcactt ctatcgatct tccagttcga ttcgatcggg gagactatgg agtgagacgt
      241 cacaaccgca agcgcgacgt gttcgcagcc agcgcagcgg gcgaggatgt gcgtcactct
      301 tgcatcgggt tgcgcgggag cttctcgtcg agcatagccg tctgataaac gaacgtcgcc
      361 atgataatcg cagcctgctt aaggtccggc acggatgccc gcccgcgaac gtcgatgcag
      421 ctttagtgta agtcttcgtt acatgattgt cggaggccga cttcatgcgt acacccaggc
      481 gcggtaccac gcagagttcc aaagcaggcc tacacccact gccactgctt tcaccgtgtt
      541 gcgcgccacc caatcggcgc tgcgttcgcg cagccaccac aagcaccccg tgaaccgttg
      601 ggccgcgtgc accgggccca gccaccagtg gcttagtaat ttgcgctacg tattttcgcg
      661 aagctcgtcg ccccggtgtc cgtatcggat aaaaagcctg tcattttatt tgcccccgac
      721 acgctcaaga atctgcacct ctacccgcag tcccgttccc agaagccgag ctgcggttcc
      781 ccgctcctca agctggtcgg cgtcttgaac ctgaccagtg gcgcgctcct ggattacctc
      841 aaaggtcaca agcaccaaca aaagcgccat tctgttcagt gtgtagtgcg aaagatcagg
      901 ccgcttcaca agccggcgaa tgcgggttag catggccgca tgccgaagac tcctctcgct
      961 gcgattgtgt tcgagtgcga tcgcctgttg cagcctgacc ggttccagga ctgggacgga
     1021 gcgcagaacg ggcttcaggt cgagaatcgc gggggcgtga cgcgaatcgc cgccgcggtg
     1081 gatgccacac gcgtcacggt gcgactggcc atcgagacgc gtgccgacct gctcctcgtg
     1141 catcacggtt tgttctggtc tgcgcgccat ccttggaccg gccggcacta cgaactgctg
     1201 cggctgctgc tcgatcacga cctggccgtg tacagcgcgc atctgcccct cgatgcgcat
     1261 ccgcgccttg gaaacaacgc gcgactctgt gccgcgctcg gattcaaacg catcgagccg
     1321 ttcttcgaat gcaagggccg gacgattggt tgtgccgcgc gcggacgcat cggacgggcg
     1381 gaactggccg aacggttgcg cggggcggtt ggggggcccg tgcgtgtggt gcccggcggc
     1441 ccggcgatct gccggcgcat tggggttgtc agcggcggcg tcggcgccga gttaagcctc
     1501 gcggccgccg gtggtgtcga cacgttcatc accggagaag gcccgcactg gacgtatggg
     1561 ctggcggagg agctggggat caacgtgctc tacgccggcc attatgcgac cgagacattc
     1621 ggggtcaagg cgctcgcggc gcacctgggc aggaagtttc gtctgccctg gtcattcgtg
     1681 gatcatccta ccgggctttg atccgcatgg cagtcatcct gcgagatcgg gccgccctgt
     1741 gcaccgacgg ggtgtagaac aagcgggtta gcggttcttc atgtagatgt agatgaggcc
     1801 ccagaggatg gccacgaaga acgccagcag tgccaaaaaa cggttgcgcg ccacgcgctt
     1861 ttcgtaccgc aacggacgca acccgtgaat gctgcccgcg gcgagatagt tgacgagttt
     1921 cggattcgac gtcggagtgc cgcgaatctg tgacagccac ttgcggagcg tggtcatggg
     1981 atcgtacttg cgcacgccca gttggttgaa gtggtcggga gtcgagtcgc ggtccgccgc
     2041 cccttgaaca ccgagatggt cgatcgcctc gaatctgggc tcgcgcgacc ccgccgggat
     2101 gcgagctgcg aaagatgcgg attgcgcggt ggggctgcca ggtcgagatg cggtgggcgg
     2161 ggcgctgagc cgctcgttga gcaggcgcag ttcaccctgg agcgcctcaa tcctcgcgtt
     2221 gagttggcgc tcactctcgg caatcggatc cggtttcttg cggaagaacc gcatggtcgc
     2281 tgggactcga aggcgtgtcg ggtgcaagga ttactggcgc agcagcagca cgcaaaacgt
     2341 gatcagcgcg atcaccccga ccaccgccag cggccaggtc agggccaggc cgacacgaca
     2401 gagttcgcgc agaggacgcc gggtccagcc gctgagcgga tcgtcggccg atgcggcggc
     2461 ggacgcctcg cccttcggcg cggcggagtc gtcgaggagg gtccatttca accgcgcggc
     2521 gttatactcc atgcgcgcgt tctcgatgct ggtgagggcg cgcgtgagtt cgttgctcca
     2581 ggtctccgtt gtccaggctt tttcgttgat cgattcgatc ctggccagcg cttcccgaaa
     2641 gccggacagc gtcttggcca gttgctcggc gtcgcgacgc gcctcgaact ccgccttctc
     2701 gagcagccca atgccgcgcg tcaaatgccc ggtcgtctcg gtccgcccaa cttcgaactc
     2761 cacccgccgc cgccgcgcct cttcgagggc tgagcgttgc cgttccaatt cctcctgggc
     2821 ccgcttgagt tcggcgatgc ggctctgtgt ctcgccgacc tgcacttcga gttcctcccg
     2881 cgtgggcgga cgagtcggca cgggagaccc ggcgatgggc ggcgcgcccg ccggactcat
     2941 ccgcgcggct cgcccgatct ccacatcacg atccacaaag tcggtctctt cgaacggcga
     3001 cgtcatagac gcactgtaac accggccttg ggtgtgtaaa ggccagctcg ttgcgcggag
     3061 acaatcgcgc gttgaccatg accgcccgcg ggtcgtaaac tcgcgtcgcg ttttcctgcc
     3121 aggcccagag tcgatcgatt cgaagccggc gcctccggcc gtcaacggcc cgaggccgag
     3181 tctcgacgcg gcggctccga actgagcggg cctgacaaac agactttcat gaaaccaatc
     3241 ctgcgcgttg tgcggggcca gccttcatgg cgggtggcct cggcggatgt cgaagccttc
     3301 gtcactcgga tggggggcca gatcggaccg gtcaccttcg accgccgcgg gcgccgggtg
     3361 caacccttcg ccgtcgctcc ctgggccgag gaggaagagg cggctgcgct gccgccgatt
     3421 ctgcgcgcgt tgcgaggcga tttcttctgc ctgccatttg gcggcaatgc caagccattc
     3481 gggaaggagc gccatccgct tcatggcgag acggccaatg cgtcgtggac tttggaatcg
     3541 ctcgagaaga aaggcgcggc ctcgatcctc catctgagtc tcagcaccaa agctcgccgc
     3601 ggtcgagtcg acaagttcat cacgctggtg gacggccatc aggccgtcta tgcgcggcat
     3661 cggatcagcg gcatgaaagg ccccatgtgc ctcgggcacc acgcaatgct gcggttcccc
     3721 gaccatgcag gcagcggcac ggtatcgacc agccccttcc tgctcgggca ggtgctgcct
     3781 gaacctaccg aacgtcccga gcagcgcggt tattccctgt tgaaaccggg ggcggagttc
     3841 gcctccctcg agaacgtgcc caccatcacc ggagagacgg ccgatttgag ccgctacccg
     3901 gcgcgccggg gcttcgagga cctcgccttg attgtcagcg actcggcggc gccatttgcc
     3961 tggacggctg tgacatttcc gcgcgagcgc tatgtgtgga tcgggttgaa agacccgcgt
     4021 gtgttgcgct cgacgctatt ctggttctcg aattgcggac gccactaccc gccctggagc
     4081 gggcgccatg tcaatgtgat gggactcgag gaggtgacgt cctatttcca ctatgggctg
     4141 gccgagtcgg cccgcaagaa caccttgtcc cggcgaggct acgcgacctc gattcggctc
     4201 aacccggatc gcccgctcga agtcaattac atcatggcgg tctcgagcat cccgtccgga
     4261 ttcgatcgcg tggcggctgt ggatcgcagc ccgagggcgg gggagggcct gctggtgcga
     4321 tcggccagcg gcaagactct gacaatcgcc ctggatatcg acttcctggg aggcggcaac
     4381 gccgggtctg aggtctccaa tggaccatct ctctcggagg gccgatcccg ccgacgcgct
     4441 tgaatctgaa cgatcttatg aaccaaaaac cacgcattca gcgcggcaat gccccgcgct
     4501 tctcgttcgg ccttcgtttt tcaacagcca gtctgtttct agccctgatc gtcggttcga
     4561 cccttctggc ggcagccgcg gccggggcga agccatcgag cacgtggcca caggactact
     4621 cggtccggcg cgatgacgat tcgggcgctc tggtactgac cacgcccttc tacacagtgc
     4681 gccacgacct gcggcaaggc ggcgccatct gccagatcac cctcacgcac ggcaaggcga
     4741 ccaacctcct gatggccccc atcgagaccc gcgtccgcac tgaccgcgat gcggtgtttg
     4801 ccgacgtgca tgaccgggcg ccccgcgtga cgcatcgccg ggaagggacg accgagatcg
     4861 tgaccgtgga cggctttttg gcggacgaga aggggcgccc ctcggacgtg cgcacacgca
     4921 cggtcttcga ataccactgg ggatacatca aggtgcgcaa ggaactcacg agttcgggcg
     4981 gagccgttcg cgcgcgcgag cttacgccgt tcttcacgcg tttggcgccg aacctgtgcg
     5041 aatacggcta tcgcgaaggc acgaccgagg aggaaggtgc gccgcctttt tcatttggca
     5101 gcaaccggtg gggcaaactg cgcaaaggcg ttccggccga caagcccgtc gagacgcctt
     5161 acgcgccgcg ctcgatgatg tttgccgatc cgggcgtcga agggctcgag tggtttgtgg
     5221 gctccgacct ctcgcaatgg gagacccagt gcacaggacg ccgcggccag ggccggtgtc
     5281 atctcgcccg cggcacggaa ccggacggga tggcggtgtc gatctcgcca ctctggatgg
     5341 cggatgcgcc cgtgacgttg ccggggcgcc tggtttttga cttctacatt ggcctgcctc
     5401 tgctcgaagg ccgcgctctc aagccgtggc ttcacacctc gttcaatcgc aaccggggcg
     5461 actgggtgag tccggatcaa atccagcgct ggaaggactc gggcattcag acggtccact
     5521 gccacaatga cggcgattat tacggcgacg gcctcttctg gcgcgatggg tcctatccgc
     5581 cctaccccga catggaccgt tacgaccagg tgctcgacgc gtgccgcaag aacgggattc
     5641 gcgtcgcgac ctacttttcg aacaaggaac tccattcgag caccagagag tttcaagagc
     5701 gaggaaccga atggggtcgg aacgaccgca aaggcaatct ccaacacaat ttcttcaagg
     5761 agaagagcga gttcggggtg cagatgtgcc tgcgctccgg ctggatcgac ttcctcaagc
     5821 actcgatcga ccgcgtgctc gacaaacacc cgctcgacgg ggtctattac gattggaatg
     5881 tcgccctgtt ctgctccaac ccgagacacg agggcaagga gcccggcgcc gtcgcggcgg
     5941 gccattggga catcgaagaa ctgctcgatt tgatggaatg gactcgccga cgcgtcgggc
     6001 ccgacggcct ggtcatcgtc cacaacacaa cgactccgat gttcgtgacg gagaacttcg
     6061 ccgattatgt ggtggccaat gaatggggct acggcagttg gtccaagaag gcgccggaac
     6121 tcaaggacct tccccttgaa tggtccctgg tcggagcccg gtcccgcggc gtgatcagct
     6181 acggtcagat cgacgccaat gcggacaggc atctccaccg gttgttcgct ctgtatgccc
     6241 tggtcagcgg cgtcacaccc tggccggcca gccccgagac gttcacgctc ttcgcccccc
     6301 tgggacaaat cgaggacatc gcctcgcatc gctttgccga ttggcgcaac acagccgtcc
     6361 gcctcgaagg tgatcgctgc gcctcagcgg tctacagccg ccccggccaa tgctacctgc
     6421 tcgtggccaa tctcgatccc gtccctaaac aggtccgctg cgcagtccga ttggaccaac
     6481 tccctcacgc cctgcccgcc ccaaccagcg ccacgctcgt cgcgtccgga ctctcgagtc
     6541 cgaccgattc ggaccccacg gttggaagcg ccctggacgt gggcagactg acgggcgagg
     6601 gtattgccat tccggttccg ggcgacagca ccgtcctcat ccgtctgcgt tagcctgcat
     6661 ttctcgccaa ctgctgaagt catctgaccg gtcacagtca cgtttcatct gcaatgcagg
     6721 ctctcagtcc cggaaatctc acccgcagac actccggaat gccgcgatcg ccccactggt
     6781 gagatttccg ggttaggctg gactatcgca catcggcgcc cgtgccggcg ccaatcgcgc
     6841 cggcgcgcgg catccgcagg ctgaactcga tccgctcgcc ttcgcggccc acgtacccgc
     6901 cccagccgac gcggatttca tcgatgcggc gccggtccag tgtcgcgtcg ttgtcgcttg
     6961 accagcctgc gagttgcagg cgatccaggg ggagataaca acggtcgtag cccgccgcgg
     7021 agagcgagcg cggtgtgctt gccaagaaat ccccgccgcc gcgttcgtgc agtatgacga
     7081 gcaattgtgt cggcgtgtgt tgtccctcgg gcacccaact ctcgatctcg aggagatcgg
     7141 cggcgctcaa gtccaaggaa tcctcaaatg gcaaacgcac aaacaggaat gtgtcaacat
     7201 cgcttcgggt cagagtgccc tcgactcgcc atcccgggcg gccaggtcga gtcgcctcgc
     7261 tgtccgccga cattgtctcg cgcacgaact cgccccgagc caccgctgct tgggcggcgg
     7321 gaagctcctt gaatgtcatc gagggggaga cgcccggatt cagcttcctc gccggtagcg
     7381 gcggaccgtc gaaccgcagg aaggtcgccc catacggcgg caagaccagt tcggtggcgg
     7441 tcttgcccgg ccacaccgaa cgctgtccgg tggcggggtc ccactgttcg gccgcgcctg
     7501 aagcccggca ctcgatggct ccctgccagg gcacggggct gtcgttgatg acaaagtaga
     7561 cttcgtggcc gtcgactcgc cgatgcgtca gacgcaacgg cgcgcgcggg ttcggcaccg
     7621 ccaaatccgg ctcgatgatc tgatcaagca cccggggcaa cagagcctcg atgcccgccg
     7681 acagaaagac gcctgtgccg cgcccgacgg ctgtgcgcgc gatcgggccg tcacccgtct
     7741 ctccgaacgc acgctcgccc caggcccgga tgcggcggtc ggggaactcg gactccgtat
     7801 tggcggggcg cgcgcccagg gcaatcacgg tcccgccctg gcgcatgaaa tcatcgagcc
     7861 gatcccaggc agcttcgggc agggtgtcga ttcccggcaa caccaccacc tgccaccgga
     7921 gggcgttgtg cgccaactcg ccttccagaa cccgcgcctc gatgagcgtg cggctgtcga
     7981 tcactgtcag gtcgcggcgc gacgcgaaca gactttccag cgcgttgcga tagagatgct
     8041 cgatccgatt ggcgccaggc gatgcattgg cccagtgctc cgccggaatg aagcgcgtcc
     8101 aaaggctctc gatcggatag acaaccgcca catccgccac ttgatggccg cccgcaagca
     8161 gcgttgcaca ccgcccaacc cattcgttca gtcggcgcag ggcggcgtca tccatccctg
     8221 tgaacgagta gtagctggtg attacgttga caccgcccgt gaagagccga ttgcacgtcc
     8281 cgcggatttc ggcctcggtc acgacgcgct tggcacgcgc gtcgcccgcc ggacggtaga
     8341 cctggccgtg atcggatgtt tcgcacatga caaacgggcg gcggtcaagc tcggccgcgc
     8401 tgcccgccag ccgcgcgatg tgccacggga cctcgggcgg aaggcttgtc agacagtcaa
     8461 tgccgggcgc atcgaggcgg cgcaggcagg cgaagaaatc gccatagagc gccacgtgcg
     8521 cggtgagccc ttcctcggcc agcagatggc cgcccgactg cacgccgtgg cgccggcacc
     8581 agtcctggat ttgcccaaag aagttctccg ccaccaactc gccgaccgtc tgccagaaat
     8641 cgtggcgatg ccgctccact cgaggtcccg ctcccaccag caacgacggg atggcatcct
     8701 cgagcgcgta tccgtgccgc ttgcgaaact cggcgctcag ccccgtcgac cagggcagcg
     8761 gccgatatgg catccgccgc aggaacaggc tcatcaacga cggttcatcg gtgaaggtgg
     8821 cgacgaagtg acggcccagg tcaggcccca tttccttcgc gtagcgggcg tgagtcagtt
     8881 cgagaaaacg ggcggtcggg gccgcctcga gcaggttcgg atacggcatc ttccgccaca
     8941 gattgccgtc ggcatgggtg ccttcgtaga gaggcgcttc cgtgacgccc aggagtctcc
     9001 aacgcccctc gggcgccgtc catgacaccc gtccgtctcg cacatgggcg ctcagatcga
     9061 cgcgcccgtc gaagtcgaca tctccatccc gcaccgggta ggccgccgcc agaaagagac
     9121 gtccgggggg cagttcgagt gtcgccggac cggcgcccgt ctcggcgtcc ttcgccaata
     9181 acccccgcgc ttcccactcg ggatggtccc gcaacacgag cccgccggcg ttggccgagg
     9241 gatagccctt ttcgtcgtac agccacaacg ccatcccggc ttgcctggcc gcttcaacag
     9301 cccgcgtgaa agcagcccac ttcgcctcgc tctcgagata attctcgaat gcgacattgc
     9361 agaccactcc gccgaagccc tggttctgca gtctgccgat gaggtcgtcc tgccggcccg
     9421 gctcatcggg ccagccgtga atgatcttga gaatgcgggt ctgcgcgggc ggttgaacaa
     9481 accgttcggc aagcccggcc ggggcgtccg gagcctgggc cgcctggccc tgcggagtcc
     9541 tgagcgcgaa agccaggctg caacaaagga ggcaagtcag cgtgaagaaa cggagatttc
     9601 gagcggaatt tatagatagc attgacattc aacgacttaa tgtacacgat gaccatgaac
     9661 cggtgcggag cggcgacgcc tgcctgccgg caggcaggcg tcccccctcc acggttcgga
     9721 tcatgaaaac tgcatttcgg cgacgtaggt ctcgtggaag tcagggtctt cgttgagcgg
     9781 aacatggcga atcttccggc gcaacgcctc atactcgaag ccgtcgccgg attcccggaa
     9841 caacgccagc ttcgctccga gcagcgccgt gtttcccgcc ggacgcaccc gatcgggcgg
     9901 caatggaagc aagccgatgc gccgggcgct ggcgcggttg atgtagttgc cgaaagcgcc
     9961 tgccaggtgc agacattcca aatcctcgag cccggctccc cattgggcca acagcaatcg
    10021 aaaaccggcg gcgatagccc ctttcgccag ttgcaactcg cgaacatccg tctgcgttaa
    10081 caccacgggc ggcgccaacg tccatcgatc gcccgatgcc aaccgccccg tcgggcggac
    10141 cagtccgagg tcgaggccgc atgccacggc gtccaccagg ccgcttccac agatgccgcg
    10201 cggagcgccg cctccgagca cccggcactg cattgcgctg tcccgcaacg cgacctcggc
    10261 gatcgccccg gtggcggctc gcatgcccat ggaaattcgc gcgccttcaa acgccgggcc
    10321 cgcagcggtc gaggcgcaga ggaggcggac gcggttccca atgacaatct ctccgttcgt
    10381 gcccagatcg accaggccca ccaagcgatc gctttcattc acctgcgtcg ccacaatgcc
    10441 cgccaagacg tcgctgccca cgaaaccgcc cagacacggc aggaaacaaa ccgtcgcatt
    10501 gccaagtccg tgccagccca aatccggcgc ggaaaaggtc ttcaatgcgt cttcgggcgg
    10561 atcgaacggg tgatgggata gaggttcgac gctcaagccg cagaacaggt gatgcatcac
    10621 ggtgtttccg accaggacaa cgcgttcgag cggcgccccg ggcggggcct cggacatcaa
    10681 ctccgcgagc aagcctccga tctgttcgcg cacgagcgtc tgcaaggtgg tctggccccc
    10741 ggccgacatc gcgaaatcga tgcgactcat gatgtcggcg ccgtgcttgg cctgctgatt
    10801 cagccccatg cgcacgccca gcacgttgcc cgtgcgccga tccaccaact gcgcgacgat
    10861 ggtggtcgtc ccgaggtcca cggctgcgcc caacccgtcg tgcggcgtga aggcgaatgt
    10921 cgagttgtca ctgaggatcg agagttccca ctgagccagc tcgatgcgca aatcgctgcg
    10981 ggcatgcgcc cggcaggcga gccgccatcc ttcagccagt tctttggacg tcagcacgcg
    11041 cttgtcttcc ggcgtcaccg gcagaccccc ttcgagcact cggacacggc agcccttgca
    11101 gcggccgcga ccgccgcacg ggaactcgac cccttgcggg aagagcgcat cctgcagcga
    11161 tgtgcccgga gcgaccgtca atcttcggcc caacggcagc aattcgatcc gcacggtgtt
    11221 gcccatgcta acgccaaatc atgcagttac gctagttccg gccccgctcg atgacaatcg
    11281 ccaaatccgt tccaccacgc ccatgcccgc cccaggtgtg aacatggcga acgagctcac
    11341 cgaccgccgt cccacaggcg cgggagcacc ggcatcgaat ctggcgtccg attaaaccgg
    11401 cgcgcccgaa acggagagac tgagcggcgg ttcagcgact ggttggccag cctcctcatc
    11461 gtgctatggg caggaaccac acaagcccac ttccgtggcc agcgacggat gagcctctca
    11521 tcgcggccaa gagccagtcg aacagattgc cgttcgccgt gctgttctgt tgctgttctt
    11581 ccgcgcacac ggacgatttc cccgcaccct ggacgagata agggtctggt tcatggcgtc
    11641 ctagatgaat cgcgccgttg ctggatgcgt caacattact gtccgcggcg cgctcagagt
    11701 gcaggctcac cggggcggct tggccccgtg cgcccgggag agaccatgcg aatcaccgac
    11761 gtcctcgtgc gaaacgggct gctgacggcc gagcaaatgg ccgacgtgct gcgtttgcag
    11821 caggcggagg gattgcgtct ggaccgggcg atcgtgcggc tcgagtacct gagcgagcgg
    11881 gagcttctgg gtgcgttggg agaacatctc aagctcaagg tggcggacct gtcgcagttg
    11941 agcgtcggcc aggaggtgct gcggctgctc cccgcgaagc tcgtgtttcg caagcgtgtg
    12001 tttccaatcg gccaggcgga cggggcgatc gaggtggcga ccagcgacgc gtttgacatg
    12061 agcgcgctgg acgaggcgcg actgctgacc gggctcgagg tgcgcccggt gcttgcggcg
    12121 gcggaggaga tcgacaagct gatcaaggcg cactatgggg tcgggggcga cacgattgac
    12181 gagatggtgg cgggggaggc ggggggggtg tcggcggaat cggcgacgag cgaggatttg
    12241 ctcgagatgg cgcaggaggc gagcgtcatc aagctggtga acgagatcat catcgaggcg
    12301 gtgaacgagc gggcgagcga catccacatc gagccgtacg agcacgagct gtcgatccgc
    12361 tatcggatcg acggggtgct gcacgaggcc tcgatgcctc cgcagattca ccggttccag
    12421 gcggcgatca tcagccggct caagattctg gccaacctga acattgcgga gcggcgggtg
    12481 cctcaggacg ggcgcatcga gttcagtgtg ggcgggcgcc agatcgacgt gcgtgtgagc
    12541 gtgatcccga tgctttttgg cgagggcatc gtgatgcgtc tgctcgacaa gtcgagcgtg
    12601 ctcttcacgc tgacgcaact ggggatggac gacgccacgt tcagccggtt tcagggcctg
    12661 atccggcgtc cgcacgggat cctgctggtg acggggccca cgggcagcgg caagacgacg
    12721 acgctctatg cggcgctcaa cgccctggtg aactccgaga tcaaggtgct caccgtcgag
    12781 gatccggtcg agtaccatct gcgcggcgtg aatcaaatcc cggtcgatcc gcaggtcggg
    12841 atgactttcg agcgcgggct gcgcgccatc ctgcgtcacg atccggacgt gcttatgatc
    12901 ggtgagattc gcgacctcga gacggcgcgg gccgcgattg cggccgcgct gaccggccac
    12961 ctggtgttgt ccaccctgca cacgaacgat gcggcgtcgg cgccgatgcg gctgctcgat
    13021 atgggggtcg agccgtattt ggtgagcagc acgctgatcg gcgcgatggc ccagcgcctg
    13081 gtgcggacga tttgtccgaa gtgcaggacg accgatccgg cggatccgcg gaccctgccc
    13141 gaggatctgg gctggaggcc gggcgatgtc ctccatcgcg gggccggatg cgaagcctgc
    13201 cgcggcaccg ggttccgcgg gcgaacgggg ctctacgaac tgctcacgat caacgagccg
    13261 ctggccgagt gcatcctgca ccgccgtcca ccgcccgaac tcatccgggc cggccgggag
    13321 gacgggttgc gcctgctgcg ggaagacggc tggatcaagg tgcgccaggg ggtgaccacg
    13381 gtggacgagg tagtgacctg cacggcggtg tgaggcatcg aggaggcgca tggccgattt
    13441 ccagtacacc gccaagaccg ccgcgggcga gaccgtcacc ggcgtgttgt cggccgagag
    13501 cgaggcgacg gccctgcgaa tcctggacga acggaatctc tttccgctgg cgttgcggaa
    13561 cggttcggat gcggccggcc ggcaggcggc gcgggggcgg gtccgcaccc gggacgtggg
    13621 gatcatgtac gggcagttgg cggatttgat cggggccggg gtgccgatgc tgcgcgcgct
    13681 cgattcgctg gtcaagtcga cggtcagcgc gcgtctggcc ggtttgttgc gggaggtgcg
    13741 ggcggcggtc gcggacggca agtcgctcac ggaggccttg cgggagcatc cggaggcctt
    13801 tccgccgttg cacaccgcga tggtgcaggc aggggagcgg gcgagttttc tcgaggaggt
    13861 tctgcagagc ctctcgggct ttctcgagcg actggacgaa ttgcgcagca aagtaatcgg
    13921 ggcgctgatt tatccggcgt tgcttgccgg cgtgggcacg ctggtcctgg tcggcgcgct
    13981 gctcttcttc gtgccgcggt tcgagccgct gctgggcgac gcgcgcaagc cgttgccgac
    14041 ggagctggtc ctttggctga gcggggtgtt gcgcgggtat tggtatttgg cgctggcggc
    14101 cggggcgggc cttggggccc tggtctgggg ggcgctgtcg agcgagcgcg ggcggcgctg
    14161 gtacgaggag tggcgcctgc gcgtgccggt tgtcggcacg gcgctgaggc ttctggccat
    14221 cacacggttt tgtcggattc tgggcacgct tttggccaat ggcgttcccc tgctgcaagc
    14281 cctctcgatc agccgcgggg cgaccgggtc gaggctcttg ggcgaccgca tagccgatgc
    14341 gatggagagc gtgcgtgggg ggcgcccgct gacggagcca ttgcgggtgg gcaacctgtt
    14401 tcccgagcag attctggcga tgatccgggt ggcggaggaa tcgaatcgtc tggacaaggt
    14461 gttgttgaag attgcggaca cggtcgagcg gcggaccaac cggcaggtgg accaggtggt
    14521 gcgtttgctc gaaccgctca ttctctgtct ggttgcgatg gcgatcggga ttctggcgct
    14581 gggtttgctc ctgcccatat tcacgatggc cagcaacctc gggcgggcgt cctgacggga
    14641 ggatgagctg aatgcaaaca ggtttgacgg cgtctattct cggtaaaata ctctgctcct
    14701 atgaagacgc gcaatctggc cgggcgaatc gcgccagtgc gggccccggt gggtttcacg
    14761 ctcatcgaag ttctgctggt gatcgtcatt atcatgatgc tggcggcggc gttggtgacg
    14821 tttgtgctgc cgcagcaggc gggggcggag cggaacacga cgcggctgtt attgacgcag
    14881 gtgcaaacct cgctcgacac gtaccgcatg aatgtggggc actatccgac cgaggaggag
    14941 gggggattgg gggcgctgct ggtcagaccg cagttcgaga acgaacaact cagcgagcgc
    15001 tggcaggggc cctacatcaa acccggcacg aaactgttgg atgcctgggg caatcaggtg
    15061 gtttacgaat tgcaggacat cgcgcgcacg ggccagacca acggcctccc ttaccgcctg
    15121 ttttcaatgg gaccggacgg ccaggcgaac accgaagacg atctggtgct ggaaaccacg
    15181 gcggacgaga acctggactc ccggagtgtg ctcgagacaa cgccgagata ggctgccgta
    15241 cgtgtcaaag accaactgcc aggcgggtta cacgctcctc gagttgctgc tcgtggtgct
    15301 cttggtgctg ctgttcaccg gcgcggctgt catgaatgtg gcgccgctgg cgcagggagc
    15361 gcggctcgac gaaggggtcg gacagctcga gacgctgctg cggtttgccc gcgccgaagc
    15421 cgcccaacag gggcggcggc accaactcca gatccaggcg gcatcggaag cccagccggc
    15481 cagccgcgcg gcgatcgcgg tgcgttggga gcccgagcct ctgcaagatc ccggtgtctt
    15541 cgtccaaagg cagtccaccg ccgcgctggc ccagtccgtg accgagcacg tgcgcatcga
    15601 gcatgtgcgc ttgatcgagc cggcgtcccc gtccgcccac ccgccagccg ctcgggacgc
    15661 atgggacatg tcggcgccgg cgtccgaacc tcccggggct cctgcgtttg gggcggtcga
    15721 ggaatggccg tcgatcacgt tctatcccga tggatcgtcc gattcggcgg agattctcgt
    15781 cggctcgacg gacatcaacg atccgcgcct gatgttggtg aagtggaacg gactgagcgg
    15841 cagcacggcg cgcgagggag tgaatcccga gagccttggc ctggttggga gcagaaccgc
    15901 cgaggcgcaa cccttggagc cggcctcggt cgcccatacg atcctcgggc cctcggctcc
    15961 ttgacctatg gggcgcgcat gcagttgcct ggaccggcgg accagactca gcccgggcgg
    16021 gagccgcgct ccgggcgcgg ggcggtcctc ctcgaggtcc tgctcgccct cggtttgttt
    16081 gtggtcgccg cggcgatcgt cactggcggc ttgaacgccg ccgtcgagcg cacgatccga
    16141 ttgcgggcgc agacccacgc gctggatctt gccgcatccg tgatctccga gattcagatg
    16201 ggactgcggg ctgcgcaggc cgcagggccg gaatctttcg aggccccctt cgatcaatgg
    16261 acctgtgaga tcgaggtggg cccgtacacg ttcggaaccg agaacgcagc cggattgcaa
    16321 ctggtgacgg tcatcgtgcg cggcgagacg ccgcccatgg tccaacgctt gacgacgatt
    16381 ctcgtttcgg agtcagggga ggcgccgtga ggatgagacc gcttcgtgtg cgggctgtgc
    16441 cgctgggtgc cggggcgttt acgcttctcg aggtgttgtt ggccctgtcg attgccatcg
    16501 gcctgctgat cgtcgtcctt tactactacc agcaggccgc cctgctccgg gacagcacgc
    16561 tcgactcggc cacgggactg gccgccgtgc gtctgtgcat ggaccgcctg ggaacggagc
    16621 tgcggacggc cagcgcgcag cccgacagtc tccgcggcag ctctcaagag atcgagttcc
    16681 ttcagtgcga gcttcccgaa tcgggcaatg tggcagcggt cgacgacgac gcccctgtgt
    16741 caaccccggt tctgctccgt cggattcgct atgccatgcg cgcgtccgag gaatcgagtg
    16801 cagcgggggg attgattcgg acggagaccg tggtgacgcc cgccgcctct ggatcgggag
    16861 aaccggagcc ggactctgtg gaaacagacc cggcggaaac gggcgttgtg gaggagtcgt
    16921 cggaaccgcc tgacaccaac gacgtgtggg cggtcgagac tgcgagcgtc gaggaagagg
    16981 gcacacgcat caccacctcg ttcccgagcg cgggcgcgcc gccgctgacc attcccgaac
    17041 ttcgctattt gcgcctgcgc tattgggatg gcgcgcagtg gcaggactcg tggtccggtt
    17101 cccatctgcc ccgcggcatc gaggtgacgc tcgccctcga gccggttccg tcggatgccg
    17161 cctcggacac gctgcccgag gaggtgtttc ggcgagtgat tgcgattccg gcggggctcg
    17221 cttcgagcgc ggctccagcc gtcgccgacg actatgaatc ggggagcgtg aacgggggga
    17281 tgaccccgga gggtggccgg tgaagacgca ctgctatccg cgcaaagacc gagcgaggcc
    17341 gtggcgatca gggggctttg ttctgctgat cgtgttggtg gtcgtgttgc tcagttcgat
    17401 gctggcggcg agtgttctct acgcgatgcg gtccgaggcc gccgcccaca ctgcgagtgt
    17461 ccagcgcgag caggcctggg cggcggccat gagcggggtg gcccgggcgc tggccgtggc
    17521 gacggatcca gggggcgact ggatggggtg ggaggataac ccggcgatgt tccagcatca
    17581 gtttgtgaag caggacggcg atgaccagtg gtttttcacg gtgtactcgg cctcggattc
    17641 cctgggcgcc gaggtgcggt tcggtttgac ggacgaagcg tccaagctgt cggtgcgaca
    17701 tcacgatccc gcatggctcg gaaagctgcc ggggctcgat gcgaagctcg ccgcggcgct
    17761 tgcgggcgcc gtcccgcgat ccgaaaccgc gccgcggacg aacggcccga gagctgcatc
    17821 atgggccccg gacgtctctg gtcgggcggg tgcgaacgat ctgccgccgg acgcgcggtc
    17881 ggacgagacc cggctgtcgg gggcaccggt atcggagggg cagccgtcgg aggctgcgcc
    17941 ttcgatcccg gcggcgtcgg ccagcccggt cccatccatc gaggagtggt ttgccgcggc
    18001 agggttgagc agtcagttgc tttacgggga ggacgcgaac tacaaccttc ggttggaccc
    18061 gaacgaggac gacggggaga cgcgcaagcc gctggacaat gcggacggcc agttggatcg
    18121 gggtttgcag cagcatctca cgacgatcag ctacgacctg aacgtcaaca gcgatgggca
    18181 gccgcgcatc aatctcaacg atgccaaggc cgacctctcg aagagcggcc tgcctcaggc
    18241 gacactcgat tacgtagcgg ccctgcgccg cgccggccag gcgctttccc acgtggccgc
    18301 attgctcgaa gccgaaggcg agtttcagga tgccgagggc cgcccgcagc gtctgcgctc
    18361 gggcattgga cgcgaggaat tgtccgtcgt gttggaccag tgcaccacga ccaatgccgc
    18421 gcgacttgaa gggctgatca atgtgaacac ggcgccgcgc gcggttctga tcgcgattcc
    18481 gtttgtgggc gaggccctgg cggacgcgat cctcgcgacg cggcccggtc tcacggcgga
    18541 cgaagcgcgc acacccgcgt ggctgtatcg gcgcggcctg gtgaccgccg aggagttcaa
    18601 gcagattgtt ccgcacctga ccacgcgcag cctccaattc agcttccgct gcctgggtta
    18661 tgccgtgccg tccgggcgct atcgggtgtt gagcgtcatg gtggacacgg cgccgcatcc
    18721 gccgcgtgtc gccgccctgc gggatctgac gcggttcgga ttcccggtgc cgctggcggt
    18781 gctggaaaac ggaggcgcgg ggggcgtgtc gacaacgagt gcgggtgggc ggctggcgtg
    18841 gtgagtcttg agtcatggcg ctcaacctcg atcacatctg gactggtctt gcggcccgtt
    18901 tgaaccggcc gacagcgtcg cgccggagtc ggcgcaccag catcacggcc ctcgatctcg
    18961 aagggggctt cctcgaggtg gtgcaggcgg cgtcgcgcag cggagagccc cgggttgtcc
    19021 ggcatgccgc tgttccactg gatcggccgc cggacgccga ttcggagacg gggtcggttt
    19081 cgggtccggt gtttgccgcc gcgctggcgc gcgctggaat tcgccctggc gccgtggtca
    19141 tgggggtgcc gcgcgcgcgg gtgatgctgc ggactctcga cttgccgccg gccgaacgcg
    19201 aggcggagat cgcggcgatg gtgcatctgc gactgcagcg cgacttgccg ttcggtctcg
    19261 acgaagctgt gatcgatttc agaactctga agcatccagc ctccctgcag gctgagccga
    19321 ccgcgccgga ccgcccgggc ccgcaagggg cggcgacccc ggtccgcgtg ctggccgccg
    19381 tggtccgacg cgacacggtt gagcactacc agtccctggc caaagcggcg ggagtgaagc
    19441 tggcgggctt gggactgcgg tcgatggcga gcgcgcgcgc agcgcaatgc ctggattctc
    19501 gaacgacgca agggtgtgtc gccctgatct ctgtccatcg acgcgaagtc ctgttcgacg
    19561 tgttgttcga gggcgcgttg gtgttcagcc aggttggggc cttggcggca ggcgcggggc
    19621 ccgcgcgggg acctgggccc gcgctggtcg agccaacggc cgaatcggta ttgaccgagg
    19681 ttgtgcgcag cctgcacagc tacgaaggcg ttgcggatca ccatccgatc gggcgttgcc
    19741 ttgtgacggg gtcgaccggc gccgagccgg gcctggcgca ggcgctggcg agccagttgg
    19801 gggtgcaggc cgaggttctc gagcccgcgg ggccgtggag tgaggcgtcc gacaacgccg
    19861 agtcgccgac ccatgcgctg ccggccatcg gtctcgccct gggcgcgctc gaacccgagg
    19921 ggttgccgat cgatcttctg aatcccaagc gccctccggt cgcgcgtgac aaccggcgcg
    19981 cgcggcaatt gggtattgtg gcggcgattc tggcggttct gctggcggct gccggcctgc
    20041 gggcgcggtg gataggggcc cgggagcggc accgcgccca actgcaggaa cagatcacgc
    20101 tcgggtccaa gaacctcgcc gcctaccggc atgtccgcaa ccaggcccgg accctcgccg
    20161 aatggtcgga gaccgggcgc aactggctcg atcacctgac tgtcttgagc gccctgctgc
    20221 cgcccagtcg ggatctctat gtgaccgcgc tttcggcaag cgcccgcaac acgctcaacc
    20281 tcgcggtcaa ggtccgcggc ggtgagactg tggatcgcct gttggaaacg ctgcgttctg
    20341 ccggctacca ggtcaaggcg cccgccatca tgccggcgat ggaccgcttc ggattccggt
    20401 tccaggcgaa cctcgaactg ggcatcccaa ccgcgatcac aaacaatctc gatgccctcg
    20461 cggtcgaatc gcgtccaccc cattcccccg ccgcctctga ggccgcggcg tcggctccca
    20521 cgccccaggc tgcggctccc acaccccagg ttgcgttacc cgcgccgccg gctgcgtcac
    20581 ccgcgccgcc ggctgaggcc tctcacggcg caccaccggg cgccgttccg gctgcggcgc
    20641 cggcatcggg ggcctggagg gagcagcgtg gatcccaggc ggggggtgag ccgcgaatgc
    20701 gtcgacgccg tccggaagga ggctggcgtg aataagcggg tccgacttct tgcctgggcg
    20761 gcgttgggcg tggccgggct gctggccatg ggttttgtgc tgagggctgc gatcaatcgt
    20821 cccctgcgca ctctcgatgg gcagattctg gcgctcgaat cgcggttgaa atcgctgcag
    20881 aaggagagac aggcctttct gaaggcggac accgaagtgc gggccggcgc cgcccggctg
    20941 ttcgccagcc aggccgcgga ggcggaggcg cgacttggag ccttgttgac ggcccagatc
    21001 gtgcgcgtgg gcttgcgcga agccgatttc acccgcgtcc ccgtcggacg ccgccgcctg
    21061 ccgggcgcgg aggaggtggg atggtccgtg cagggggagg ggtctctggc gcgtttgctg
    21121 gatttgctct ttctgttgca gagcgagccg cggctgcaac ggatcgaggg tctctcgctg
    21181 tctcccgcca atgaaggcga ccgtacgcgg attcggtttc ggtatgcgac attggtattc
    21241 ctgccggcgg tcgagatcga ggccgcgatc cccgaggcgg aactgagcct cgatgcgccc
    21301 ctgcgccggc gatacgacgc aatcacgcag cgcgatctct tccgtccaca ctcgccccag
    21361 gagcgcgccg cgaatccgcc gcctgcgggt gcgccgacgc ctgagtcgga acagccgcat
    21421 ttgcggatcg tctcgctctc ttcgtgggac gggcaaccgg aggtgcacct gttcgacgcg
    21481 cggcaacagc ggctggttgt ccgccggccc ggcgatgaac tgctcgacgg cgaggtggcg
    21541 gcggtggact accgcccgca gcccgtgccc ggcaaggccg gcctgatcag ctacagccgc
    21601 ctcatctggc ggatcgacac caactactgg gcggtcgagc cgggtcagac cctggctgac
    21661 cgacgcctac tggcgccgga agaacttccg gcgacgcttc acccgattcc cacacacccg
    21721 gttctaccat gagactcgat cactgcatct gtttggtctg tttggctctc tctagcctgg
    21781 cgccgctgcg accgctggcg gccgacggcg cgggcgccga cacgcctgtc ccggccacaa
    21841 actcggcgcc ggacgccgtc ccgatcgatg ccggcgccgc gacgccggcc tccgtcaccc
    21901 cgccatcggg cacagcgacg aacgcgccgc ccgacgcggc cggcatccgg tttgactttg
    21961 ccggcatgcc ttattccgag gcgatccagc gcttctgcca gatggccggc aagccgttga
    22021 tcgccgacac ggttgtagaa ggcgcgctga cgttttcgga tccgaagccc tacacatacg
    22081 gggaggcgct ggacacgttg aacctgatcc tggcgaccaa gagcgtcatg ttgatcgaag
    22141 acgaccgtta cctgcgcctg gttcccttca aggcgttgtc ccaaatgccg ctcaagctga
    22201 tccgcgggct cgagaacgtt ggcgatgtgc gccccggcga actcgtcacg gttgtgttgg
    22261 atctcaagaa tctggatgcg ggcgaactct cgcagcccat cagcgccatg ctttcgtcag
    22321 ccggttccgt ggcgtcgctc tcgcgcgggc gcgggctgat catcaccgat cgccttgcga
    22381 acatccagcg cgtccggcaa ttgctcgacc aggtggacac ggcctcgccg gtccagcggc
    22441 aggtcaagac gttcgccctg cgcaacgtgt ccggcacgtt gatgagcgac ctgttgaacc
    22501 gcacgttcgg catcgccacg gccccgaagc ggaccgtgtt caacgagcag cgcaaggcct
    22561 acgaagtgct gccgcccgac cccaccgatt acgtgaccgc gatttttgcc gaggcctcga
    22621 acacgctggt gctgttcggg ccgtcggaac ggatcgacat ggccggcgac ctgattgccc
    22681 gtttcgagag cgatgcgggc gccgccgcga gcgaaatcaa ggtgttctac ccacaaatgc
    22741 ccgtcgagga actggccgaa atggtccgcc aggcgatccc gggcgtcgct gggcgaggcg
    22801 atcgcgggcg cgagagcgac acccgcgcca aggtgatcac cgatcggacc agcaaccggc
    22861 tgattgtgac ggcgccggcc attgggcaac tcgacgccat caccgcgctc attcgccgtc
    22921 tcgatccgtc cactcaggag acgcccgcgg agaaggcccc cgaccgttcg acgccgcagc
    22981 gagaactgcg cgtggtggat ctcaagtcgg gatccgccca ggctttgacg ccgatgctcc
    23041 gcgatgccct gaccgatctg actcgggaca cacccggccc gaatccaatg agccaggtgc
    23101 gaatccaggc cgcgccgatg gggaaccgga tactgctgac cggcgccccg gccgccctcg
    23161 acgacgtcct gcgcctgatc gagcaactcg acacggtgag cgcgccggcc gaagtgacgc
    23221 gcgtgttcaa gctgcgccag gcaaacgccg gccgcgtcgc gggcctgatc atgggcgcga
    23281 tgggcggata cgactggcgc cgccgcggcg gcggcggtgc agtgctggct gccgcggacg
    23341 atcgcagcaa ctcgctgatc gtcgcggctt cggaaggcca gatgaagatg atcgagcagg
    23401 tgatcgaaca gctcgaggcg gtgggcgcgg tcggcgggcg gcaattgaaa atcctcgaac
    23461 tcaccaacaa ctcggccgcc acggtcgccg gcacgctcgc gcggttgttc gccccccaag
    23521 tcaactcgta cgatcccacc cagcgcgtcg cgctcgctcc ggcgcccaat gaccgcgccc
    23581 tcgtgctcga agccgacgag tcgatgatgg cccggctcga ggaggcggtg cgaacactcg
    23641 atacggccgc cgcggccgga ggcattgaaa cgcgcgtcgt cgaggtcaag tcgggatcgg
    23701 cgcaggccct ggccccgatg ttgaacgagg cgctgtatca gtttgggcgt gacggccggg
    23761 gcgccagccc gttcgggcgc gtccaggtgc agtcctcgcc cgtgggcaac cggctgctgc
    23821 tcgccggcac ggcggaggcg ctcgaccgcg tcgagaccct cgtcgcccaa ctcgacagcg
    23881 tcccgacccc ccaaagcacc cgagtcttca agctgaagaa cgccgatgcg cggcaggtgg
    23941 ccaacatggt cctgagcaca atgggcgggt acgaatcgcg ccgccgcggc ggcggggcag
    24001 gctacgtggc ggccgccgct gaagaacgga gcaattgcgt ggttgtgtcg gctcccgact
    24061 cccagctcga actggtcgag gacatcatca accggctaga cgaggccggg ctcgcgggcg
    24121 ggcgcaagtt ggaaatcctg ccgatcgacc gcaactcggc ggcgtcgatc gcggcgatgg
    24181 tcacgcagat gttctacccg cagatgcgca gttccgatcc ggacacgcgg atcgcgctca
    24241 cggcggcccc ggacgaccgt gcgctggtga tcgaggctgg cgagtcgatg atcgatcggc
    24301 tgcgtgagac tgtgcaggcc ctcgatgtcc cgcccacgct tggggggatc gaggtgcgca
    24361 cctacgcact gccggagggg cgatccgtcg agctggcgga ggccctcaac cgcatcttcg
    24421 ccgacccgtc ggaatcccgc cgtcgctggg atcaaccgat gtcggccgca ccgccggcgc
    24481 cgcggttcga gatcgacagc ggctcggaac tgctgatggt ggcggccacg gccgaccagt
    24541 tcgagaggat cgagaaggtc cttgacgatt tgcgcgcggc cgcagcggcg gcgtcgcaaa
    24601 tccgcacctt ccgcctccag catggcgatc cggcccaggt ggtcgaggtt ctgcagtcca
    24661 tgctcttggg agacgccagt caacgttggc gcagctcgcg cggctggcgg ggcgggaccg
    24721 gttcgggcga ggtccgcgtg gccgccgcgc cggcgctcaa cgcggtcgtc gtgcaggcgt
    24781 ccccggagaa gctcgcgctg gccacccagc tcattcgcac cctcgattcg ccccgcagcg
    24841 aggacgccgc aagcatccag acggtgcggg tcgagaaggc ccagcccgag gcggtggccg
    24901 ccgcggtgac gcaaacgctc gccacgcaga gtccgcccaa caccgcgccg cgggtgagcg
    24961 tgacggccgt gccgggtggg cgcagcctgc tgctcagcgg cccggaagcc gaagtgcaga
    25021 ggattgccgc cttgattcgg gaactcgacc aagacagtcc ttccgaccag atcgagacgc
    25081 gggtcttcca gctcgagcac ggcagtgcgc gcgagttgag ccgcgtgctc acgcagttga
    25141 tcgaggggct ctcgcgggcg cgcccgcgtt tcggcaatcg gttccagcgg gacagcaccg
    25201 tcacgattgc ggctgacgag cggacgaact cgctgctggt gtcgggctcg gcggactcgt
    25261 tcaagctggt ggagcaactt cttgtcacgc tcgaccaggc gccgcaacac tcggatcgcc
    25321 aggtgcaatt ttactggctg aagaacgcgc gggcctttga cgtggcgctg aaggccgagg
    25381 ccctcttcgc cgagcgcccc cgggccgaac ggccgctcat cgagcccgat tcgttttcga
    25441 actcgctgac catcgttgcc aagcccgccg atcttctcga aatcgagagc gtcgtgctga
    25501 agctcgatga agccgcccgc gacaccagcc tccaggtccg aatgatcccc ctgaccgacg
    25561 tcccggtcga gcgcatggcc gccatgctga ccaacctcta ctcgcagctc aacgagggac
    25621 aggtccggct cgtcgaccga ttgcccagcc caacgccacg tccggctccc tctctctccc
    25681 ccgtcaccaa cgcgcctgcc gccacaccca cccgacccga cgcgccggcg gccccggacg
    25741 cccctccgtc gccggcggcc cctccggagg tggtcatcgc cgtggacaag gcggccaacg
    25801 cgctgatcgc ctcgggtccg gcctacgagc tggatcacat cagttcactt gtttatcagc
    25861 tttcggcctc gatcgcctcg ccggagttcg agatacgcca gttccgcctc aaggaagccg
    25921 accccgtcgt ggtcgcgcgc gtgctgaacg aattgttcaa gccggttccg acgcaagctc
    25981 agggacaggc gggccagccg ggcggcgcca cgccccgacg gggcgcggac gggcgcagag
    26041 aggccgagag tcaaggcggg gggcgtgtcg acaaccaggg gcgctctcag ggccagccgc
    26101 aggtcgtcgc gccgaaggtc gccgtggttg cggaatcgcg cacgcacagc gtcatcgtgc
    26161 gcgcgcgtcc ggcggatttc gctttgctcg aatcgatcat cgagcaattg gacgagggcg
    26221 gattgacggc ggaactggcg caccgccttg tgcccctcga gcacgtgacg cccgaacggg
    26281 tgttgccgct ccttcgccag atgctgaccc agttgaccag cgcgcgtccg ggcgagccgg
    26341 cggccattgc gggcgatccg cgcagccggg gcatcttcct ggtcggccgg cagagcgtgc
    26401 tggaccgtat cgaggccttg atccacgagt tggacacgtc gtccgagttc gccgagatcg
    26461 agctgcggac gctcgatctg aagcacgcgc aggcgcccgc ggtggcgggg ctgatgaaga
    26521 gcgtcttgcg ccccgatgcc tcgagcgaac tgagcgccga agcccgccaa ttgcaggagc
    26581 agatcagccg cttgaaactg ctcgatgccc aggggcgtcc gatcggtctg gatctgaccc
    26641 agccgatcaa gatcgtggct gacccggccc aacccaatgc cgagggtggg aaccggctcg
    26701 tcatcggctc gacacccgga aacctcgatg ccctcgccgc cgtcgtcgcg ctcctcgaca
    26761 cgccctccgc cgccggggcg gaatcgcttc gcgtgttccc cctcacccat gcggacgcct
    26821 cgagcatcct gcaggtgctc aatgacctgt ccacccgcgc cgcgggcgcc ggacgccccc
    26881 gccccgagga ccgggcgaac gtgtcggttg acgagcggac caacacgctg ctcgccgcgg
    26941 gcgccgccaa ggccctcgaa cgcatcgccg aacttgtcga gcaacttgat cgcgaactgc
    27001 ccgacgacct gcgcggcatc cgcatcctcc ccctcaccca tgccgacgcc acaaccatcg
    27061 cctcatccct ccagcgactg ctcgatgaac gggcccgacc ccggggcgga gggcgccgga
    27121 tgggcccatc ctcgcgcgtg gccgtgattc ccgatgcccg gagcaacagc ttgctgatca
    27181 gcggcggtcc ggacggcttc gatcttgtcc agacgctggt ggcgcagttg gatcagccgg
    27241 gcacctcgct cgtcggccag gtgcggctga ttcccctgga gcactccgat gccgggacgc
    27301 tcgcgacaac gctgagcgac cttttcaacc ggcgctatca agccgcacgg acgcccgaca
    27361 tgcagcgcaa ccggcccgtc atcctggccg atccgcgcag caacagcctc ctggtcgccg
    27421 ccggggtgga cgacaaccag gccatcgacg ccctgctggt caagctcgac cgcccgccgg
    27481 agaacccgtc ggtggaactg agcgtcctcg gcttggagta caacgactcg gcccgcgtgg
    27541 cggtcacaat ccgcagcgtg ttcgccgccc ggcttcggag catgacgccc gtcggccagc
    27601 agcccgcgcc ttcggatcgc gtcgaggtcg aggcggacac cctcagcaat gcgctggtca
    27661 tctcggccag caagccgaac ctcgagttgg tgcgcgagtt ggtggccaag ctcgatgccg
    27721 agccagtggc caccggaggc ctcatcgaga ccttcacgct cgaacatgcg gatgtgcagc
    27781 gtgtctcgac cctgttgcgg tcgctgctcg atcagggcct ctaccgcccg ggtttggcgg
    27841 ttgaaggcgg gcgccgcagc cggcgtgacg cgctcgccct ggccaccgat ccgcgctcga
    27901 acacgctgat cgtctcggcc agccccgaga atctcctgat cgtcaaggaa ctcatccgtc
    27961 aactcgatac cgccgatttc accggggcca gcgatatccg cctcttcgca ctcaagcgcg
    28021 cgcgcgcctc gcacctggcc acggtgctcg aacagttctt ccgcgcccga cgcgccagcg
    28081 agaccgccgt gggcgccgcc cagcggtccg ccccggtcac tgtcacggcc gacgaccgca
    28141 ccaacacgct cctgatcacc ggcggcaagg agagtttcga cgccgtggag cgaatgatcg
    28201 agcaactcga caccgatacg gcaatggcgc gcaccaactt ccgggtgtat gaactgcgtc
    28261 acaccaccgc cacgaagctg caggccacac tccagcgcct cttcaccaac cgcccccccc
    28321 gcatccaggg ccagccgccc gaaccggtga ccgtcattgc cgacggctgg gccaacgccc
    28381 tgatcatcgg ggcgtccgat gacgatcttg ccatggtcgc ctcgctcatc gagaagctcg
    28441 ataccgagca gccggccggc ggcgcgcagg tccaggtgtt tcagatggcc aaggcagacg
    28501 cgcggcgcgt gcaacagacc ctccagtcgc tcttcagggg cagtgcgggc gccggggcgg
    28561 ctccgggcgc cgcgctggtc ccggtggtgc tcgatgtcga cgagcgcctc aatgccatca
    28621 tcgtctcggc cggcgagacg gatctcaaac gcatcgccga cctcgtcaag aagctcgaca
    28681 cggaccaggt ggcgcgtgtg gctgaaatcc gcattttccc gttgcagaac gggcgtgccg
    28741 tcgagatggc cctggtgctc aacaacgtgc tgaacaacaa tccccgcgcc cttaccgagg
    28801 ccagtccgaa ccggcagtcc ctgctccagt tcatcaccaa aacacaggaa ggcgagcggc
    28861 tgatcaccag cgcgctcaag gaaggcattt tgatcgtgcc cgattctcgg gcgaattcgc
    28921 tggtggtgtc cgccccgctc gattacatgg cgctgctcag cgaggtcatc tcgcacctcg
    28981 acgccacggc ccccacgatg gcgcagatca aggtgtttca tctgaaaaac gccgatgccc
    29041 gtcagatggc ccaggtcctc accagcctgt tccgtctcca gcgggtcggc gccgcgggcg
    29101 ccggccagca gtcggtgcaa tacgtcatgc ctgccacgac ggacgcgacc ggtcccgagg
    29161 gcgaggcgcc ggccgaagcc atcggcgcgg tggtcggatc cgcccaggaa gacgcccttt
    29221 cggtaaccgt cgacttccga accaacagcc tgctggtggg cggcacggaa cactacgtcg
    29281 ccctcgccac gcaaatcatc gccaccctcg attccacgcc ggcgcaggag cgcacggcca
    29341 aggtgtaccg gctcaagaac gcgcgctcgc aggccgtcga ggcttccctg cgcaccttcc
    29401 tccagcagga tctccagcgg atcatgtcga tcctcggccc ctccggcgtg ggcaccgcgc
    29461 agaacatcct cgaccgcgaa gtcagcatcg tgtcggagac caacagcaac agcctcctga
    29521 tctccgccag cccgcgctac ttcaaggaag tccagggact ggtcgaggaa ctcgatcaac
    29581 cccagccgca agtgctcatc caggtcctgc ttgccgaggt cacgctcgac tcgaccaccc
    29641 aactaggcgt ggaatgggac tacacgtccg acaccgaccc tgtggtgagc accgggacgg
    29701 attttggaca gcgggagctc ctccaggact acggcgggta ctggtcttcc gtcaccggca
    29761 gcaattaccg gtttctggtg cgcgccctcc agaaccaggg ccggctcgag gtgttgagcc
    29821 gcccccagat tctcactgcc gacaaccagg aggccacgat caacatcggc caaagcgtcc
    29881 cggtcgtcca aagcacccag gtcactccgc agggcggaac ggtcagcgcc atcgagtacc
    29941 gggacgtcgg tgtcatgctc accgtcaccc cgcggatcag tcccgacggc tacgtgaaga
    30001 tggacgtggc gccgagcatc tcggatctct cgagttcgaa ggtcaccatc tcgaaggggg
    30061 aggacttgcc gatcatcaac cagcgcaccg ccaccacgac cgtgaccgtc aagagcgggc
    30121 agtcggtcct gatcggcggg ctgatcggga ccattgatga cgagcgaacc aagaaggtgc
    30181 ctcttctggg agacatcccg ggtctggggg tcctgttccg cagcaagagc aaaggccaaa
    30241 cccgcaaaga actgctcgtt gttctgaccc cgcaaatcct gctcaaggga gaaggcgagg
    30301 gcagaagctt cgacgcccag accttcaccg aagaggaact ccggggatcg accatccagg
    30361 gcgagttcca gcgcgatccg cttcagcgcc gtgtgctcga tcctctgctg cccccgaaaa
    30421 ccctcgaaag ccagactccg cagtgaggag agttagcgtt tcatgggtgc cccgcgttct
    30481 caacgaagaa acagcaattc tcacgaatga tatcgatcca attcggaatt cataacggcc
    30541 tgcggcgagc cgcgggggct tgcgcggcct gcctgcttgc cgtggccctc tgcggctgcg
    30601 tcaccaacag caagacgccc cgcgcggctt cctcgtcggc catcgacgag ttgaacctcc
    30661 tcgggctgcc ggtcgcggtc aatctggacc gcagaccggg tgcggacggc gtggtggtga
    30721 agctctttgc cacaagccga tcgagcccgc gcgccatgcc tctccgatcc ggcacactcg
    30781 aactgatcgc ctatgaaggc acgcccagcc cagccgcttt gccgatgccg ttccatcgct
    30841 ggacattcac cccggccgaa ctggcccata acgagttcac cacggtgcta gggacgggct
    30901 atcgcctggt tctgagctgg atgccccgat cgctgccatc cgatcgcgtc acgatcatcg
    30961 ctcgctacca cccgcacaag gccgagcccg tgacgtcggc tcccagctcg atcaccgcgt
    31021 ccgcgtattg aatgcggatg cgggacgaat cttcgagacc cgggattcga tcagactggc
    31081 ttgcaatcga ggcggaagga attactatgc ccactgcgat tgcaagccac tcccaggata
    31141 gcgagagggc tcggacctaa acaccattat gtcagacgac cggacgattt atgtaaccac
    31201 ggccgacatg gcccgccttc aggcgctgct ggcccgccag aagaacgctg gtgaggccct
    31261 cgagaaactg gccgccgaac tcgatcgcgc ccgggtggtc gcccccgagg atatcccgca
    31321 ggacatcatc acgatgaact cgacggcgca gttgcgcgaa ctgctaaccg acgacgtgat
    31381 gacctacacg ctggtctttc cggaccgggc tgattacgag gcagggcgca tctccgtgct
    31441 cgcgcccatc ggcaccgcca tgctcggcca gcgcgagggt gacgagttcg agtgggaggt
    31501 cccggccggg cctgtgcgtc tgcgggtcga gaaggtgatc tatcagcctg aggccgccgg
    31561 acgctctgat ccgtagagcg aattcagggt ggaccaccgg agaaacaagg gactgcatct
    31621 ttccgttctc cccttagggt ctgtgcggac ggcaagatct ggcagaccgc ggcttccgcc
    31681 tacaaccccg acggtaagcc atacgcgtgt gatctgtccc tacttttagc gataggtttt
    31741 ggataaagac cggaaatgac ttgtggaaag gcaggtctgg tctactttca ccgatttgaa
    31801 agaacacgac atgcattcta accttgataa cggaccggtt ctcaccaaga cacgcgaact
    31861 gtgcgagacc atcatcaacc agactgattt ccttgccatg cggcgggacg tcgagacgtt
    31921 tctggctgac gatgatgcgc gctcccggta ccacagcctc gccaacaagg ggcaggacct
    31981 gcaatacaag cagcagcagg gtgctcagtt gacacgcgag gaaatcgagg ccttcgagca
    32041 ggagcgggag accatcatgc agaatggtgt gatccgcgga ttcctggaag cccaacagca
    32101 gatgcaacgg gtccatgaga ccgtcggccg ctatgtcatg aaggcgatgg aactcggtcg
    32161 cgtgcctgaa gaggacgatt ttgccgggtg cggccagggc tgcagttgcg ggcactgagg
    32221 cgcgccggtg cgggaagttg gggttggcgg caggccggat gcacagcgtc cgccaaattc
    32281 ctctttggag agccggaagc ggcgcagttg taaccagaag tcggagggcc gagttccacg
    32341 aggcactgca ggttccgtag ccgtcccgca acgcgggatc agtccgcgcc atctcgctcc
    32401 gtcggaggca ggaatccgcg ccgactcatc ccgcaatgcg ggacggcgac aaaccggttc
    32461 atggtcccgg tccgcattca atcggtgaat gtcaacgctt ccacgattag ggtctggttc
    32521 agcgcgcagc ctgggatcga tccgggctat gggcttggct cggctttttt ggtcgccgcg
    32581 gcttcgagct ttcccacttc ctcgatcctg gctgcggctc ctgcgtggcc ttgcttggca
    32641 gccgcctcga accatttcct ggcttcctga agattcttct cgaggccgtc acccgtcaga
    32701 tatcgcttgc caaggtcgaa ttgggcgacg gctgacccct gttcggctcg tttcttctgg
    32761 aactcgatgg ttcttttaag agtctcggcc ttctgatctg tctgtttctt gagaatctct
    32821 gcgtcgaccg tgttggtctg gggtcgggcg ggcggggtcg tgcccggcag aggcgggggt
    32881 gttggagaga gagtcgccgg cggtgtggcc agttgggtct gaggggcgat cggtttgaca
    32941 tagcgccgcg caccgtagaa ttgggcagtc cagctacgtc cggggaggat gtcgatccag
    33001 ccggcaggca aggtctcagg acagagccag ccgccgacca cgcacacgag cgccaggcgc
    33061 ggagtccatt tcatgacgct ggaaaagtcc cacatttcat cgcatcggtc aacctatccc
    33121 agtgctttgc tccttgttcc cggcccagcc gcgattacac cgccttgatt tgtgtggggg
    33181 caacggaggt ttcgacaaag gttggctgcg cggcaggcaa tcacggttgt tctccggtgt
    33241 ctctcacgct tcttgacgtt ggcgccggct caccgcagcc tgcgcccatg aacgccttcg
    33301 caaagggttg tccgggcccc ggcgccccgc gtaccagctc ccaggcctgt atgaggatcg
    33361 ttgcccttgt ctgcctcgcg tcgagtctct ggcgcaatgg cgtccaggcc gggggggagg
    33421 atggctggct gactggcgtc gacacctggg gcgggcgggc ggcattgccg gccgtggtga
    33481 atcccgcggt cgcgtcgccg ctgcaatccg tgctttcgct ccgtggcgaa tgggaattca
    33541 ccacgcgccc ggtggccccg ctgcgtcatc cgggctggaa cgccttctac gcccagccgt
    33601 ggcccgatgc gcgtacgatt caggttcccg ggtgttggga agcgcagggg gtgggggcgc
    33661 ctggcatggg ggattcctgg gactgcaagt gggatcattg tgcgaagccg ctgcgcgcgg
    33721 tttaccgggg ggacgcgtgg tatcgcaaga cagtcgccat tcccgaagcg tggcgcgggc
    33781 agcgggtctg gctcaaggtg ggcggcgtgc gttctcaggg ctggttctgg gtcaaccgga
    33841 cacccgtc
//
LOCUS       DAOQRO010000003        23248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1435_length_23248_cov_5.091666, whole genome shotgun sequence.
ACCESSION   DAOQRO010000003 DAOQRO010000000
VERSION     DAOQRO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 23248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23248
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_23248_cov_5.091666"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKK20_00250"
     CDS             complement(<1..499)
                     /locus_tag="PKK20_00250"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNU98353.1"
                     /translation="MDTSKITADHVRARKGGARIPALTAYDYPTTRLLDEAGIPLILV
                     GDSLGMVVLGYPDTTAVTMEVMEHHTRAAARARPRALLAADLPSRSYRTPAEAVVNAR
                     RLVAAGAEAVKAEGGLAIADQVRAIVAEGIPFLGHLGMLPQHVLEEGGYHIKGKREHE
                     RKALLE"
     gene            604..1704
                     /gene="recF"
                     /locus_tag="PKK20_00255"
     CDS             604..1704
                     /gene="recF"
                     /locus_tag="PKK20_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147771.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNU98354.1"
                     /translation="MHLAHLRLRDFRNYARLDADFPPGFHLLLGNNAQGKTNILEAIY
                     LLATLRSFRGVGGAQMVRHGQKGYFVGASVVGPGTREIKLYWSASERRISLNGQPIRR
                     LTDYLGAVRAVVFCTEDQQLVKGPGSRRRRFLDLLLSQTAPAYLPLLQRYLRALRSRN
                     ALLKQRVTDEAALDSFTREVVAAGNEIMRWRREFTPRLSPLVRLAYRKISNGAEDLRL
                     DYQPSAKVDFAVELAQARSRERIFRTTVCGPHRDELRLLLDEKPVAQFASEGQKRTLA
                     IALKMAQAEYVTGLHGAPPILVIDDVMGELDAKRRSGLLPLIERSHQASSQVFMTCTE
                     ENWPRELGRQLHRWEVRAGTLIRIEDSIRGPM"
     gene            1928..3244
                     /gene="serS"
                     /locus_tag="PKK20_00260"
     CDS             1928..3244
                     /gene="serS"
                     /locus_tag="PKK20_00260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNU98355.1"
                     /translation="MLDVKLIRDQRDLVRERLASRGGGDEAKVDEVLGIDAQRRQALA
                     EVEALKAQRNRVSKEIGALMGQRKLAEAEARKAETRQIGDRIAELDGKVAALEEARDQ
                     VLLRLPNLPHASVPAGRAAEDNVVVRMWGEKPVSVFPLLPHVDLCSRLKLVDFGRGAK
                     LSGSGFLLYTGWGARLERALISFLLDLHTGQHEYTEVSPPYLIGRDCMVGVGQFPKFE
                     DQAYAVQEGLDSSTLGKLYLLPTAEAPVANIHRDEILLEAQLPIRYVAYSPCFRAEAG
                     AAGVGTRGMIRVHQFDKVELIKIVRPEDGYAEHEKMVADAERVLQLLGLHYRVVLLCA
                     GDMGFASAKTYDIEVWAPAQGQYLEVSSCSNCEDYQARRMNCRYKLADGGNRFPHILN
                     GSGTALARLFVALIETHQQADGSVSIPEPLRPYLGADRLPLPACCP"
     gene            3241..4692
                     /gene="glgA"
                     /locus_tag="PKK20_00265"
     CDS             3241..4692
                     /gene="glgA"
                     /locus_tag="PKK20_00265"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNU98356.1"
                     /translation="MRILLASSEIFPYSKSGGLADMVAALAKSLAAAGHQVGVVTPLY
                     AGIRERFHGLHPFDWRMQLPLGGNLVSASVWVAEPAANLTLYFIDQPGFFRRRGLYGT
                     PQGEFADNADRFVFFAKCVAHLARYLPWVPQVVHVNDWHTALTPLLIRHQALTDGWAA
                     PPATCLTIHNLAYQGVYPAQTFALSNLPWSYFTADGAEFHGQFNCLKTGLVSSDAITT
                     VSPSYAGEIQTPDHGCGLEGVLRLRAGALTGILNGVDYDEWRTTGNRFLAAPYEADDL
                     SGKDANKLALQREMGLPPMPGTPLFGMVTRLVHQKGIDLALGALEEMLAGDLQFVLLG
                     SGLVEFQEAFLELEERHPLKAAIRLGYNHALAHRIEAGCDFFLMPSQFEPSGLNQMYS
                     LRYGTVPVVRATGGLIDSVVDIRENLERANGIKFRDYSTAALAKAMRKALALYREPDL
                     LLDYRRNGMRADFSWDRSTAQYVEVYRRITRRA"
     gene            complement(4791..5960)
                     /locus_tag="PKK20_00270"
     CDS             complement(4791..5960)
                     /locus_tag="PKK20_00270"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNU98357.1"
                     /translation="MRTTYVPPLTAETAKDQTLKVSPATVPVETVQPVKGAIMLVGLD
                     FGTNTSCLKVAPLGSTQPLLSEIIPTVVGYAKEGIVDNLLPDNAKVLFGHLAQKNRMY
                     LRMVPPLAGGIVVDMAAAHDFIRHLRQLINAPAGVELRVVAGLPASADRTAREHLLEA
                     LGGFFHKALLVPEPFLAALGFRDPSKLGDPAYLDPVRNSVFVDIGAGTTDVCLVQGYF
                     PTADDQISVPFAGDKIDALLQAAIGQAYPDVQLSLFKIRELKEAHSYVGKIDSPITTS
                     VLVGGKPRKLELGEIMGDACQQLLLKILDCVRAIIAEASTDSVGELMQNIILTGGGSR
                     VRHLAPELERLLGEEGYEKPRVLTVGENYKQHVAEGAVVAGRQAKENQWISLAGR"
     gene            6250..7119
                     /locus_tag="PKK20_00275"
     CDS             6250..7119
                     /locus_tag="PKK20_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU98358.1"
                     /translation="MGKIVEPRSNARRRVCASGFTLIELLVVIAIIAILAGMLLPALG
                     KAKSKTQGIQCMNNHRQLCLAWRMYVEDNNDALLYASEDSSKPETLLYSWVTGTTDSS
                     PANRVNWDPELTVKKSPMWKYCGESLNIWRCPSDKSQFNVNGQMKPRVRSMSMNLYLG
                     GWGGTDGGWGAQVSNYKIYRKYTELIDPGPTKVFVFLDMREDSIDMGNFAVNMAGYPD
                     HPEQHGFWDLPGFYHNNGGGFSFADGHSELRRWKDPRTTPPLVRNGQVPDRISTPRNA
                     DVAFLQDIATRPK"
     gene            7297..11343
                     /locus_tag="PKK20_00280"
     CDS             7297..11343
                     /locus_tag="PKK20_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNU98359.1"
                     /translation="MSKLRCPHLAAATAAFLIVAGTAPILRSADYTSTVMALNPVGYW
                     HLDETVTVPPPRVFANAGSVGEAGTGYGVLDLLPGEEGIVGSAIRMFNAGLSTAVCGS
                     KVDVPWNAAWNPEGSFTLEFWAKPAALPVGSQSDICAISSLGNDATSSDRSGWMMYMA
                     PGAKWDFRVGALNSYAITAKGSGLVANQWQHVVAIYDGQAKTADLYVNGVRPSDLAVV
                     TTGNPYRPNPLRPLRIGGTGFNDGSLGGYSGNRGFDGWIDEVAFYHGVLSAERVAAHY
                     DKGINDKTGYGAAVMADNPVGYWKLDEPAYTAPDPGTLPVAVNAGSAGAAANGVFYPG
                     TTPGVEGPACAGMAASNKAAALSGVVGRVVIPDADALDFTGQVTIMAWVKPTSKAMLR
                     NIVTHGSEGASLSLKEIVLRINQGMYEVGTLEGVAVNTVTAAVPDGDIGNWVFLAGTW
                     DEGNGWRLYRHGEPMAANYTGFGAVTVNGGWAIGARGEEPGVPYPEADGRTFQGAIDE
                     VAIFNRALTPAEIKSVYIASKPLPIIIQAPKAPAGAVYAGDTVVFEVTAEGTPPLAYQ
                     WTKDGAALPGRTSPTLTLSNVTAGDSGTYAVEVSNANGKVTSSVNLEVMATAPEITRD
                     PASAMRYAGAAMRFSVGVTGSAPLSYQWKLNGTPIAGATSAEYVLDPVRADQAGSYTC
                     TVSNAFGEVTSAAATFAVLPAPAGYAAEVLADNPMAYWRLGETSGAVAYDFWGGLDAQ
                     YRGVALGQDGFSFLDPDKAVGTSAADTYVGGIDGAVTDFSGATATFTIEAWVKGPPNQ
                     VDGAGIIAKGTGDVGMGGDPGTEQFCLGISSGVYTFFVRQDGDFAMVSCDADVGPNGT
                     WQHLVGVYDGENGLMSFYVNGVPMGGGNPTPTYGVRPSTQPVSLGAKRGGVDASHDLY
                     FIGTIDEVAIYKTALTEDRVWAHFQAQYGTSLAPTIQVQPVPVTAYVNMPAKLSAAAA
                     GSQPMSYQWKKNGVNVAGATTDTLSFAKLQPSDGGRYTLVVQNSIGSVTSDEVVLTVL
                     PLPTADVAIPGLVAHWKFENNLTDDTGRGNNGTAKAQGTPPLAISYVDGKIGSKALRY
                     ETNIEDPANVRSCYVELGVRPDLQFGASQDFSVVFWVQVPYNYVGGDLPFLCNAVNSA
                     NSPGYTFAPSFGPGDTGVPGGWGWSLYDNAAGGINVYGAVGSINDGQWHHLVFSFDRQ
                     GLGVTYLDGLVVDSRLITSVGSLDTGNQTCIGQDPTGLYAGFEWPISFALLDDLGIFR
                     RALTPLEATSIYAAGLNGHSFVNKVVEQPTVAVQVLQDGTLRLQWEGGGALQQADQVT
                     GGFTDIAGATSPHTVAPDGTQKFYRVRL"
     gene            complement(11333..11668)
                     /locus_tag="PKK20_00285"
     CDS             complement(11333..11668)
                     /locus_tag="PKK20_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98360.1"
                     /translation="MERYADLLGKTNDAARFAGQARRMTDAFNGKHLRPAEVERPSDP
                     KVACGRNDTDARFAFWSSRLRRGHSSVKHACQAGVSDGKRNGQEPSPGRLFEPRRTGR
                     GRALRWIRA"
     gene            11789..13267
                     /locus_tag="PKK20_00290"
     CDS             11789..13267
                     /locus_tag="PKK20_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNU98361.1"
                     /translation="MNPASGAPPPTRTGHPPGLSALFFTEMWERCSFYGMRAVLILFM
                     TAAVQTGGLGMSDALAGAVYGLYTASVYFSALPGGWVADRLLGAQRAVWYGGLLIVLG
                     QFTLTLPRTDTFYLGLLFVVLGTGLLKPNVSAIVGQLYPEGGARRDAGFTIFYMGINL
                     GAAIGPLVCGYLGEKVNWRLGFAAAGFGMLAGLIQFRLLRARLGEAGAVLGQSESRKR
                     RDMAVLGGGLGILGLVVVIIWSGLVSIDALEVAQATKWFILGLAVLFFAGVVLFGGLS
                     GVEKKRVGVIAILFVCSALFWCGFEQAGSSFNLFAKRHTDRQCLGWVMPAGWFQMVGP
                     VFVISLAPVVAAIWVGLARRGLEPSLPAKFAFGLALLGAGFLVMAWGAKRAGTGLVWP
                     TWLISTYCIHTLAELCVSPVGLSSVTKLAPARLTGQMMGVWFLATSLGNLVAGLIAGE
                     VSDESTRLMATRFMQIAGLTGGLAAVLALLTRPVQRLMGGVR"
     gene            13337..14332
                     /locus_tag="PKK20_00295"
     CDS             13337..14332
                     /locus_tag="PKK20_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNU98362.1"
                     /translation="MPFDRDRMSARAVELAARGVYVGTSSWKYEGWINQLYTPERYAY
                     RGQLARSRFDRSCLGEYAQVFKTVCVDAAYYAFPGRTILQRMADQAPENFLFGFKVTD
                     TITVKKFPNLARFGDRAGKVNDQFLDADLFAKAFLEPCEAIRPKVGLLIFEFSGFWPT
                     DYSRGRDFLADLDMFLGRLPGGWPYGVELRNSNWLHRDYFECLSRRRVAHVFNSWGAM
                     PPVGEQLSLPESRTHPELVGARFLLKPGRRYEEAVQWFQPYDKIKEVNEEARVAGAAL
                     ISEGATTPGRKTFIYVNNRLEGNALETLDAMVGLVSIPSTPLQQGPRRNRLSARS"
     gene            complement(14393..16282)
                     /locus_tag="PKK20_00300"
     CDS             complement(14393..16282)
                     /locus_tag="PKK20_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNU98363.1"
                     /translation="MITPTTTNLAGLAGSSMIPEKVINLQVAPIEQVLSVYAELTGRT
                     VLRPTTLPATSVTLKNQQELSREEAIQALESVLALNGITMIDVGDKFVSAVPTQQALT
                     EGAAFSKEEAKNLAELGQFVTKIVQVTNALPSEVAQVIQTFGKIQGGVVPIDSTMTLV
                     LRDYPPNIKRMMEIIEKVDVKVESDYRLEVIPIKYGKVEEIYSTMSSVIGGGGASVGM
                     GAGGTGTLSTRRGTTGARGGSQFRSNFRQTNITGSQRQTGQYQTQAQVGQAGQLGQAG
                     AASTFNQRLQGLVSRMSAMGEGQLVVDAKIIPDERSNSLIVYATKDDMKMITNIVEKV
                     DRLLAQVLIEAIIMDVQLNDNFEFGVSTFMRQQQTGNLTSIAAAKNSPSFLAAITNIM
                     DGSGLPGGFSYFGKYSSDLDVAVKALASNGKGEILATPRVQTSHATPANFTVGESVPY
                     VTSTYYGGYGGYGYGPSAQLQQLDVQTYLDVTPYITPDGLVVMEINQNIEEITGYTEI
                     ANVGKMPNTTHRSASATVSVQDGDTIILGGYIRASKSKSKSGVPILKDIPGIGGLFRS
                     TSKEAKRSELLVLIRPTVLPSPRDAAQAVDHARENMPGIRDMEDEWKADYEKLNQRSN
                     DRRKK"
     gene            complement(16717..17367)
                     /locus_tag="PKK20_00305"
     CDS             complement(16717..17367)
                     /locus_tag="PKK20_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98364.1"
                     /translation="MASVFDKLGLRPQEQRFVVLTVTIIVIVVSVLFIFPRFKDWGRL
                     QSAKAAARTTLQTNQKEIARVPEYRSQLQKLELAGSAVLKAEQALDLTRTVQNKAQEM
                     GVAITGIRPAAQAGAGSTNQFFDEQLVSVDLSCLDQELVNFLVALGSGDSMIRVRDMD
                     LRPDPPHYRLLGKITLVASYQKTAKAAPPAATPRSGATRLAPGPTANPPSTPKGST"
     gene            complement(17372..18754)
                     /locus_tag="PKK20_00310"
     CDS             complement(17372..18754)
                     /locus_tag="PKK20_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98365.1"
                     /translation="MKTGRWNNCNVLDARQERRQLWHFGRDGSGARLLSERVVLSTDP
                     LPARHVARTWSDVWQPKLNVAWLPASQVFLRVVHLPPSDPKELEGMVELQIERLSPLP
                     ITQVVWTFEVLSPPASPAQTIVVIIATRNVVEDVLGRLEDSGFLADRLELPQLRELLA
                     IQASGDGVWLLPREEGGRAVCLAAWRFGGQWQNLSLLQLSGDEQAGQHMVDLLRQIAW
                     AGEMEGWLIPNPAWHLVAEEGLAARLESALRTAVGDALTIHPPLALPRLAALSATATG
                     AGNLVPAEFLSRYRQQFIDRLWMRALGGLGLAYLFAILGYFVALQWVQYQKDALDQQI
                     VFSTSAYTNALELRAKVQILQEQVSLKFAALDCWKAASDNLPQELNLTQLTFQRGKKL
                     GLFGTVPSDQQGLVTTYNQALAAATVNGQPLFSQVSTRSIQASAAGPSGSTARPMNWS
                     IECEIRRSDL"
     gene            complement(18763..20514)
                     /locus_tag="PKK20_00315"
     CDS             complement(18763..20514)
                     /locus_tag="PKK20_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU98366.1"
                     /translation="MALASIKTVLAQAALVTPEQCEQWHKTWRAQVESGGQDSLLTVF
                     QRESGLSEEAFLRALAGALGHTYVDRVTIPEDVPREERKLILSKISTKVAFQFTVMPM
                     RLIEDRLQVAVANPFDPAMVNAVQFEAQSPLEFALASKAEIEKALKKYYGVGAETLDE
                     MSEEESVELEIASDREITEGDQEASVIKFVNQVIWEAYKDRSTDIHLEPMEDELRIRY
                     RIDGILHQTPMQPQLKRFQAAIISRIKVMSGMNIAEKRLPQDGRINVRIKGEEIDIRV
                     STVPTVYGESVSLRLLTRGKIFLTLDKLGFLPEEEEAIREIIVKPHGIFLVTGPTGSG
                     KSTSLYAFLSTINSTQKRIITIEEPVEYELRGINQIAVRPDIGLTFAMGLRHILRQDP
                     NVVMVGEIRDLETAEIAIRAALTGHLVFSTLHTNDAPSAFTRLIDMGIEPFLVASSVE
                     AVMAQRLVRTICPFCKTEQSVEPDYLHRIAFPPEHIGTTRFLRGAGCEECRQLGYQGR
                     TGIYELLILNEALRPLILNRAASSTIAQRAMENGMRTLRVDGWRKVRNGITTIEEVLR
                     VTQTEEHLAALIEDQKG"
     gene            complement(20659..22731)
                     /locus_tag="PKK20_00320"
     CDS             complement(20659..22731)
                     /locus_tag="PKK20_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNU98367.1"
                     /translation="MGEDKTVVQPAEASPLRPLSRPAKLAPTPPGKPRYAVTIRDAAG
                     HEVVLADPRATRALVALMNVHAVNGGAACHWGGPAAFAEIMAAIHGLMFSTQGRPWHE
                     AYNFVNDAGHAENGLYALRANYGYDGLTFESLRAFRSLESKLTGHGESHLNPEGVLLS
                     NGPLGSALPQAQGLALADRLAGAERVTIAAVSDGAAMEGEAKEAFAAIPGMASKGLLN
                     PFVMVVSDNNAKLSGRIAADAFAMRPTFEAMQVLGWRVIVVDHGHDLAAVYGAIESAV
                     AQARANPRQPSCVWAKTIKGYGVKATEQSASGGHGFPLSNGEKIVEFVREIYGGEPPE
                     ALLRWALELRADWEAKEAAKKEKAAAAALQSTTPPKKDKVQSGLAKGAIRAAREGWPV
                     FSVSSDVQGSTGMSLFQKTFPDRFVDVGVAEANMISVAAGLSKSGFIPIVDTFAQFGV
                     TKGNLPLTMAALSQAPVIAVFSHAGLQDAADGASHQATTYLAATSAIPHTVVIAASCA
                     DEAEAFMAQAIQRLGEARRRGDDGESVIFFIGRESYLTSWVGGAQFEWGRAQVLQEGG
                     DVVLIGCGALVGKAIEAGRQLASEGVRATVINNPFVNRVDLDTIGGAVSACHGRVVTI
                     EDHQIMGGMGAQVAHALANAKVAHRMISLGIRGEFGQSAYLADQLYEKHGLTVNAMVR
                     AAKELIGS"
     gene            complement(22990..>23248)
                     /locus_tag="PKK20_00325"
     CDS             complement(22990..>23248)
                     /locus_tag="PKK20_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98368.1"
                     /translation="RPLSKAPLRLDPNLDGDLWFHGVEVDWGNSPLNYDDPLPWALEP
                     LPRESPHGQFRAGRHALRILGRGVGGADRSEFLLRYRIESG"
BASE COUNT         3942 a         7207 c         7727 g         4372 t
ORIGIN      
        1 cttcgagcag ggccttgcgc tcgtgctcgc gtttgccctt gatgtgatag ccgccttctt
       61 cgaggacatg ctgcggcagc atgccgaggt ggccgaggaa cggaatccct tcggccacaa
      121 tcgcccgcac ctggtccgcg atggccagcc ctccctcggc cttgacggcc tcggctcccg
      181 cggccacgag ccgacgggcg ttgacgacgg cttccgcggg cgtgcggtag ctccgggagg
      241 gcaggtcggc cgcgaggaga gccctggggc gggcccgtgc ggcggcgcgg gtgtgatgtt
      301 ccatgacttc catggtcacc gcggtggtat ccgggtagcc caggacgacc attccgagcg
      361 aatccccgac gagaatgagc gggatgccgg cttcgtccag cagacgggtc gtcgggtaat
      421 cgtatgcggt cagggccggg attcttgcgc cgcccttgcg agcccgcacg tgatcagcgg
      481 tgatcttcga agtatccacg cagccaaaca tgcgtcgcca cgagcaggga acaagcggaa
      541 aatgagattg gccgatgcca ggattgcttt gaagtcctgt caccctgcga ttacgcttcg
      601 gccatgcatt tggcccacct gcgtttgcgc gatttccgca actacgcccg gctggatgcc
      661 gattttccgc ccggtttcca cctgctgctg gggaacaacg cccagggcaa aaccaacatt
      721 ctcgaggcga tctacctgct ggccaccctg cgctcgtttc gcggtgtcgg gggcgcgcag
      781 atggtgcggc atggccagaa agggtacttc gttggcgcct cggtcgtcgg cccgggcacg
      841 cgggagatca agctgtactg gtcggcgtcc gagcggcgga tcagcctcaa cggccagccg
      901 atccgccgtt tgacagatta cctcggcgcg gtgcgggcgg tggtgttctg caccgaggac
      961 cagcaattgg tgaaggggcc cgggagccgg cggcggcggt ttctcgatct tctgctgtcg
     1021 cagacagcgc cggcgtatct gccgctgctg cagcgctacc tcagggccct gcgctcgcgc
     1081 aacgccctcc tcaaacagcg cgtgacggac gaagccgccc tggacagctt cacccgtgag
     1141 gtcgtggcgg cgggcaacga gatcatgcga tggcgacgcg aattcacgcc tcgcctctct
     1201 cccctggtgc ggcttgcgta tcgcaagatc tcgaacggcg cggaggatct ccggctcgat
     1261 tatcaaccga gcgcgaaggt ggatttcgcg gtcgaactgg cccaggcgcg ctctcgggag
     1321 cgtatcttcc ggaccactgt ctgcggcccg catcgcgacg aactgcggct gttgctcgac
     1381 gagaagccgg tggcccaatt cgccagcgag ggacaaaaac gcaccctggc catcgccctc
     1441 aagatggccc aggccgagta tgtgacgggt ctgcatggcg ccccgcccat cctggtgatc
     1501 gacgacgtga tgggcgagct ggacgcgaag cggcgcagcg gcctcttgcc gttgatcgag
     1561 cggtcgcacc aggcaagcag ccaggtgttc atgacgtgca cggaggagaa ctggccgcgc
     1621 gaactcggcc ggcagcttca tcgctgggag gtgcgcgcgg gcacgctgat ccggatcgag
     1681 gactcgatcc ggggtccgat gtgatcggcc gtgtcgaaac ggttgaatgc gcacggggac
     1741 catgaaccgg tgcgaagcgc cgacgcctgc ctgccggcag gcagggcctc gtcgccccga
     1801 actccttcaa aaacactctt cggcggggga cgagccgttc ccctcctggg ttcggttcat
     1861 tgcgagagag acttgggggt ctcgcgtcgc ccttgcccag gcaaggggcg gcgcgcatac
     1921 tcggcggatg ctcgatgtca aactgattcg ggatcagcgg gacctggtgc gggaacgcct
     1981 ggccagccgg ggcggcggtg acgaggccaa ggtggacgag gtgcttggga tcgatgcgca
     2041 acggcgccag gctttggcgg aggtcgaggc tctcaaggcg cagcgcaacc gtgtgtcgaa
     2101 ggaaatcggc gccctgatgg ggcagcgcaa gctcgccgag gccgaggcgc gcaaggccga
     2161 gacgcgtcag atcggcgacc ggattgccga gttggacggg aaggtcgcgg cgctcgagga
     2221 ggcgcgggat caggttcttc tgcggctgcc gaatcttccg cacgcgagcg tgccggctgg
     2281 tcgggctgcc gaggacaacg tcgtcgttcg gatgtgggga gagaagccgg tgtcggtttt
     2341 tcctctgctg ccgcatgtgg acctctgcag tcggctgaag ctggtcgatt tcggccgcgg
     2401 cgcgaagctg tcggggagcg gcttcttgct ctacacgggc tggggcgcgc gactcgaacg
     2461 ggcgctgatc tcgtttcttc tggacctgca caccggccag cacgagtaca ccgaggtctc
     2521 gccgccgtac ctgatcgggc gggactgcat ggtgggagtt gggcagttcc cgaaattcga
     2581 ggatcaggcc tacgcggtgc aggagggatt ggacagttcg actctgggca aactctacct
     2641 cctcccgacc gccgaggcgc cggttgccaa catccatcgg gacgagattc tcctggaggc
     2701 ccagcttccg atccgttacg tggcctacag tccgtgcttc cgggcggagg cgggcgcggc
     2761 gggcgtgggg acgcggggga tgatccgcgt gcaccagttc gacaaggtcg agctgatcaa
     2821 gattgtgcgg ccggaggatg gatacgcgga gcacgagaag atggtggccg atgcggagcg
     2881 ggttctgcaa ctgctggggc tgcattatcg ggttgtgctc ttgtgcgcgg gggatatggg
     2941 attcgccagt gccaagacct atgatatcga ggtctgggct cccgcgcagg gccagtacct
     3001 cgaggtgtca agctgctcga actgcgagga ctaccaggcg cgccggatga actgccggta
     3061 caagctggcc gacggcggca atcggttccc gcacatcctc aacggcagcg gaacagcctt
     3121 ggcccgcctc tttgtcgcgc tgatcgagac gcaccagcag gccgacggtt cggtatcgat
     3181 tcccgagccg ttgcgcccct acctgggcgc ggaccgtctg cccctgccgg cgtgctgccc
     3241 atgaggattc tactggcgag cagcgaaatc ttcccgtact cgaagagcgg gggcctggcg
     3301 gacatggtgg cggcgttggc caagtccctg gccgcggcgg gacaccaggt cggggtggtc
     3361 acgccgcttt acgccggcat tcgcgaacgt tttcacggat tgcatccgtt cgactggcgg
     3421 atgcagttgc cgctgggagg caacctcgtg agcgcgtcgg tctgggtggc cgagccggcg
     3481 gcgaacctca ccctctactt catcgaccag cccggctttt ttcggcgtcg cgggctgtac
     3541 ggcacgccac agggggagtt tgcggacaac gcggatcggt ttgtcttttt tgcgaagtgc
     3601 gtcgcgcacc tggctcgata tttgccctgg gttccgcagg tcgtgcatgt gaacgactgg
     3661 cacacggcgc tcaccccctt gctgatcagg catcaggcct tgaccgatgg atgggccgcg
     3721 ccgccggcca cgtgcctcac catccacaac ctggcctacc agggtgtcta cccggctcag
     3781 acgttcgcgc tgtccaatct cccctggtcc tatttcaccg cggacggcgc cgaattccac
     3841 ggacagttca actgcctcaa gaccggcctg gtctcgagcg acgcgatcac gaccgtgagc
     3901 ccgagctacg ccggggagat tcaaaccccc gaccacgggt gcggtctcga gggcgttttg
     3961 cgcctccgag ccggcgccct gaccgggatt ctcaacgggg tcgattacga cgaatggcgg
     4021 actacgggca acaggtttct ggcggcgccg tacgaggccg acgatctctc aggcaaggac
     4081 gcgaacaagc tcgccctcca gcgcgagatg gggcttccgc cgatgcccgg cacgccgttg
     4141 ttcgggatgg tgactcggct ggtgcatcag aaggggattg acctggccct gggcgcgctg
     4201 gaagagatgc tggctggcga tcttcagttc gtcctgctgg gaagcggttt ggtcgagttc
     4261 caggaggcgt tcctcgaact cgaagagcgc catccgctca aggcggcaat ccggctcggt
     4321 tacaatcacg ccctcgcaca ccgcatcgag gccggctgcg acttcttcct gatgccttcg
     4381 caattcgagc caagcggctt gaatcagatg tacagcctgc gctacggcac ggtcccggtg
     4441 gtgcgcgcga caggcgggct gatcgattcc gtcgtggaca ttcgcgaaaa cctggagcgc
     4501 gccaatggca tcaagttccg ggattactcg acggcggcac tggccaaggc gatgcgcaag
     4561 gccctggccc tgtaccgtga gccggatctc cttctcgatt atcggcgcaa cggcatgcgg
     4621 gccgatttct cgtgggaccg gagcaccgcg caatacgtcg aggtctatcg gcgcatcaca
     4681 cgccgcgctt gatctcccgc cgatccgggg ggcgcgcggc gccagcccgc atcgtcactt
     4741 cttttgcgtg ctccagcttc gcggtgtcca aaagtggaga gaaatgcagg ctaacggcct
     4801 gcaagagaga tccactggtt ttctttggcc tggcgtccgg cgacgacggc gccctcggcg
     4861 acgtgctgtt tgtaattctc gccgacggtc agcactctcg gtttttcgta accttcctcg
     4921 ccgaggagac gttcgagttc gggggccagg tggcggacgc ggctgccgcc gccggtgagg
     4981 atgatgttct gcatcagttc gcccacgctg tcggtcgagg cctcggcgat gatcgccctc
     5041 acgcagtcca gaatcttgag cagcagttgc tggcaggcat cgcccatgat ctctcccagt
     5101 tcgagtttac gcggtttgcc gccgaccagc acgcttgtcg tgatcgggct gtcgatcttg
     5161 cccacgtagg aatgggcttc cttgagttca cggattttga agagggagag ttggacatcc
     5221 ggataggcct ggccgatggc cgcctggaga agggcgtcga tcttgtcgcc ggcaaacggc
     5281 acgctgatct ggtcgtcggc ggtcgggaag tagccttgca ccaggcagac atctgtcgtt
     5341 cccgcgccga tgtccacaaa gaccgaattg cggaccgggt cgagatacgc gggatcgccg
     5401 agctttgaag gatcgcgaaa cccgagcgcg gccaggaaag gctccgggac gagcaacgcc
     5461 ttgtgaaaga agccgcccag ggcctcgagc agatgctcgc gggcggtccg atccgccgag
     5521 gcgggcaacc ccgccaccac gcgcagttcg acccctgcgg gcgcgttgat caactgacgc
     5581 agatggcgga tgaaatcgtg cgcggccgcc atatccacca ctatgccgcc ggccagcggc
     5641 gggaccatgc gcagatacat gcggttcttc tgggccaggt ggccgaaaag gaccttggca
     5701 ttgtcgggca ggagattgtc gacgatgccc tccttggcgt atccgacaac ggtgggaatg
     5761 atctcgctca gaagaggttg cgtcgagccc agcggtgcca ccttcaggca cgacgtgttc
     5821 gtgccaaaat cgaggccgac gagcatgata gcgcccttca caggctgcac ggtttcgacc
     5881 ggcacggtcg ccgggctcac tttgagggtc tggtctttcg cggtttccgc ggtcagtggc
     5941 gggacgtaag tagtgcgcat tgggtcattc ggttcgcgtc gatcgggcgg tctgccaccg
     6001 gccatccaac cggtgaacgg cgcccgctgg cgtccgagtg tggcgatgga cggggaaaat
     6061 gcaagcgcgt gccgcgccga aggcacgatt ttttgggcgg ccagtgtcgg tgtggcatct
     6121 ggtatgcctc ataaagccca ttgacaggat gtcgattcga gctattaaga aggcacgata
     6181 agcgcgccga gggcagaaac ggcagacggc ccgggcgggt gaaccaacct aacaactgga
     6241 ctcatggcta tgggaaagat tgttgaaccg cgttcgaatg ctcgacgacg ggtctgcgcc
     6301 tccggtttca cgttgattga actcctggtc gtcattgcca tcattgccat tctggcaggg
     6361 atgctcctgc ccgctctcgg caaggcgaag tcaaagaccc agggcatcca gtgcatgaac
     6421 aaccacaggc agctttgtct ggcctggcgg atgtacgtcg aggacaacaa cgacgccctt
     6481 ctgtatgcca gcgaggacag ttccaaaccc gaaaccctcc tctattcgtg ggtgacgggg
     6541 accacggact cgagcccggc caaccgggtc aattgggatc cggaactcac ggtcaagaag
     6601 agtccgatgt ggaagtactg cggtgagagc ctgaacatct ggcgctgccc atccgacaag
     6661 tcgcagttca atgtcaatgg ccagatgaag ccgcgcgtgc gcagcatgtc catgaacctc
     6721 tacctgggcg gctggggcgg aaccgacggt ggttggggcg cgcaggtgag caattacaaa
     6781 atctaccgca agtacaccga gttgatcgat ccgggtccga ccaaggtttt tgtctttctc
     6841 gatatgcgcg aggacagcat cgacatgggg aacttcgcgg tcaacatggc cggttacccc
     6901 gaccaccccg agcagcacgg cttctgggac cttcccggct tctatcacaa taacggcggc
     6961 ggattctcct tcgccgacgg ccactcggaa ctccggcgct ggaaggatcc gcgcaccacg
     7021 ccgccacttg tgagaaacgg ccaggtgccc gatcgcatct cgaccccccg aaatgccgat
     7081 gtcgcttttc ttcaggacat cgccactcgt ccgaaatagc cgggccttcc gcaaaccgat
     7141 ctctgacgca tttgccccgg tcgctggtcc agtcgtggat ttcagcggcc ggggttttcg
     7201 tttggctgga gaaactcctt gacagcggcg cagcggcttc ttgaggatcg tcgtcgtcaa
     7261 gcaacccaaa gaacacacat cggccggaga cttctcatga gcaaactgcg ttgcccccat
     7321 ttggcagccg caaccgcggc ctttctgatt gttgcaggca cagcgcctat ccttcggtct
     7381 gccgactaca cctcgaccgt catggccctc aacccggtgg gctactggca tctcgacgag
     7441 acggtcacgg tcccgccgcc gcgggtcttc gccaacgccg ggtcggtcgg cgaggcggga
     7501 acgggctatg gggtgctcga tttgctccct ggcgaggagg gcatcgtcgg tagcgcaatt
     7561 cggatgttca acgccggttt gagcaccgct gtgtgcggat cgaaggtgga tgttccctgg
     7621 aatgcggcct ggaatcccga gggctcgttc accctcgagt tctgggccaa gccggcggcg
     7681 ctgcccgtgg gcagccagag cgacatctgc gcgatttcct cgctcggcaa cgacgccacc
     7741 tccagtgacc gctcgggctg gatgatgtac atggcgccgg gcgcgaagtg ggacttccgt
     7801 gtgggggcgc tgaacagcta cgcgattacc gcgaagggca gcggtcttgt ggccaaccag
     7861 tggcagcatg tcgtcgcgat ttacgacgga caggccaaga ccgccgacct ttacgtcaac
     7921 ggggtccggc cttccgacct ggccgtcgtc accaccggca atccgtaccg gcccaatccg
     7981 ctgcgccccc tgcgaatcgg gggaacgggc ttcaacgacg gcagcctggg cgggtactcg
     8041 ggcaatcgcg gtttcgacgg atggatcgac gaagtcgcgt tctaccacgg agtgctgagc
     8101 gccgagcgcg tggccgccca ctatgacaag gggatcaacg acaagaccgg ctatggtgcg
     8161 gcggtgatgg ccgacaatcc ggtggggtac tggaagctgg acgagcctgc ttatacggcg
     8221 cccgaccccg gcacattgcc tgtcgcagtc aatgccggca gcgccggagc cgccgccaac
     8281 ggcgtcttct atcccggcac gacccccggc gtcgagggcc cggcgtgtgc ggggatggct
     8341 gcgagcaata aggcggccgc gctgtcgggc gtggttgggc gagtggtcat tcctgatgcg
     8401 gatgcgttgg atttcaccgg ccaggtcacg atcatggcgt gggtcaagcc gacgtccaag
     8461 gccatgttgc gcaacattgt cacgcacggt tcggagggtg cgagcctctc cctgaaggaa
     8521 atcgttttgc ggatcaacca aggcatgtac gaggtgggca cactggaagg cgtcgcggtc
     8581 aacacggtca ccgccgctgt gcctgatggc gacattggca attgggtgtt cctggcgggc
     8641 acttgggatg agggcaacgg ctggagactc tatcgccacg gcgagccgat ggccgccaac
     8701 tacaccggct ttggggctgt gactgtcaat ggcggatggg ccatcggggc acggggggaa
     8761 gagccgggtg tgccctatcc cgaggcggac ggtcggactt ttcagggcgc catcgatgaa
     8821 gttgcgatct tcaaccgcgc tctgactccg gcggagatca agtcggtcta catcgcgtcg
     8881 aagcctctgc cgattatcat ccaggcgccc aaggccccag cgggcgccgt gtacgcgggc
     8941 gatacggtcg tcttcgaggt cacggctgag ggaaccccgc cgcttgctta tcaatggacc
     9001 aaggatggtg cggcgctgcc gggcaggacc tcgcctacgc tcaccctcag caacgtgacg
     9061 gctggggaca gcggcaccta cgccgtcgag gtgagcaacg ccaacggaaa ggtgaccagt
     9121 tcggtgaatc tggaggtcat ggccaccgcg ccggagatca cccgagaccc cgcttcggcc
     9181 atgcgatacg ccggagcggc aatgcggttc tcggtcgggg ttacggggag cgccccgctc
     9241 tcctaccaat ggaaactcaa cggcacaccg attgccggcg cgacgagtgc cgagtacgtg
     9301 ctggaccccg tgcgggcaga ccaggccggg agctacacat gcaccgtgag caatgcgttc
     9361 ggtgaagtga ccagtgccgc ggcgaccttc gctgtcctgc cggcgcccgc cggttatgcg
     9421 gcggaagtgc tggccgacaa cccgatggcg tactggcggc tgggcgagac cagcggcgcc
     9481 gtggcctacg atttctgggg tggactcgat gctcagtacc gaggtgttgc gttggggcag
     9541 gatggctttt ccttcctcga tccggacaag gcggtgggca ccagcgccgc ggacacgtat
     9601 gtgggcggca tcgatggcgc agtgaccgac ttcagcggtg cgacggcgac attcactatc
     9661 gaggcgtggg tcaaagggcc gccgaaccaa gtggacggcg ccggcatcat cgccaaggga
     9721 acgggcgacg ttggcatggg gggcgatccg gggaccgagc agttctgctt ggggatcagc
     9781 tcgggtgtct acaccttctt cgtccgccag gacggcgact ttgccatggt gtcgtgtgat
     9841 gcggatgtcg gtcctaacgg gacctggcag cacctcgtcg gcgtgtacga tggcgagaac
     9901 ggactgatgt cgttctacgt caacggcgtc ccgatgggcg gtggaaaccc gacgccgacc
     9961 tacggggtcc gtccctcgac tcagccggtc agccttgggg ccaagcgggg gggtgtcgac
    10021 gccagccacg acctctactt catcgggacc atcgacgaag tggcgattta caagacggca
    10081 ttgaccgagg atcgggtctg ggcgcacttc caggcgcagt acggcacgag cctggcgccg
    10141 acgatccagg tccagccggt gccggtgacc gcgtatgtga acatgccagc caagctgagc
    10201 gcagcggcgg ccgggagcca accgatgagc tatcaatgga agaagaacgg ggtgaatgtc
    10261 gcgggagcaa ccacggacac cctgagcttc gccaaactgc aacccagcga tggcggacgc
    10321 tacactctgg ttgtccaaaa ctcgattggc agcgtcacca gcgacgaggt tgttctgacc
    10381 gtcctgccgc tcccaacggc ggatgtcgcg attcccggcc tggttgcgca ctggaagttc
    10441 gagaacaatc tgaccgacga caccggtcgg ggcaacaacg gcacggccaa agctcagggc
    10501 acgccgcccc tggccatttc ctatgtggat ggcaagatcg gaagcaaggc gctgcgatac
    10561 gaaaccaaca ttgaagatcc cgccaatgtg cgatcgtgtt acgtcgagct gggggtgcgc
    10621 ccggaccttc agttcggtgc gagccaggac ttctccgtgg tcttctgggt gcaagtgccc
    10681 tacaactacg tcggtgggga tttgccattc ctctgcaacg ccgtcaactc cgccaacagt
    10741 cccggctaca ccttcgctcc gtcattcggc cccggcgaca caggggttcc aggtggttgg
    10801 ggttggtcgc tctacgacaa tgccgcggga ggcatcaatg tctatggcgc cgtcggctcg
    10861 atcaacgatg gacaatggca tcacctggtc ttttcgtttg accgccaggg gcttggcgtg
    10921 acctacctcg acggcctggt ggtggattct cgtctcatca caagcgtcgg gagcctcgac
    10981 acgggcaacc aaacctgcat cggacaggat cccacaggcc tctatgccgg cttcgagtgg
    11041 ccaatctcct tcgctctact cgacgacctc ggcatcttcc ggcgcgctct cacgccgctc
    11101 gaagcgacat cgatctacgc cgccggcctg aacgggcaca gcttcgtcaa caaggtcgtg
    11161 gaacagccga ctgtcgccgt gcaggtgctg caggacggca ctctgcgttt gcaatgggag
    11221 ggcggaggcg ccttgcagca ggccgatcag gtcacgggcg gattcaccga catcgcgggc
    11281 gcgacgtcgc cgcacacagt ggcaccggac ggcacgcaga agttctatcg cgtcaggctc
    11341 tgatccaacg aagggctctg ccgcgcccgg tacggcgcgg ctcgaacaga cggccaggag
    11401 acggttcctg gccgttcctt tttccatccg acacgcctgc ctgacacgca tgtttcacgg
    11461 acgagtgtcc gcggcgaagt cgagaactcc agaatgcgaa tcgcgcgtcg gtgtcgttcc
    11521 ggccgcaggc gactttcgga tcgctcgggc gctcgacttc ggctgggcgc aggtgcttgc
    11581 cgttgaaggc gtcggtcatg cgccgcgcct gcccggcgaa ccgggcggcg tcgttggtct
    11641 tgccgagcaa atctgcgtaa cgttccatga gatgcgggtc aggttcatga gcgtggccgc
    11701 cttgaagttg gaaccgattc ttgaaacaac gtcttgccag ccactccgca cggtcttacg
    11761 aaatggccgt gcgaacctct ctgatgccat gaatccggca agcggcgccc cgcccccgac
    11821 tcgaacaggc catccgcccg gcctgtcggc gctctttttt accgagatgt gggagcgctg
    11881 cagcttctat gggatgcggg ccgtgctgat actgttcatg acggcggcgg tacagaccgg
    11941 tggtttgggg atgagcgatg cgcttgcagg ggcggtctac gggctttaca ccgccagcgt
    12001 ctatttctcc gccctgcctg gcggttgggt agccgatcgg ttgcttgggg cgcagcgggc
    12061 ggtctggtac ggcggtcttc tgattgtctt gggccagttc acgctcacac tgccgcggac
    12121 cgacacgttc tacctgggat tgctgttcgt ggttctgggc acgggccttc tcaagccgaa
    12181 cgtcagcgcg attgtcgggc agctctatcc ggagggcggg gcgcgacgcg atgccggctt
    12241 caccattttt tacatgggca tcaatctggg cgcggcgatc ggccccctgg tctgcggtta
    12301 cctcggcgaa aaggtgaact ggcggcttgg cttcgcagcg gccggtttcg ggatgctggc
    12361 cggactgatc cagtttcgtc tgttgcgcgc gcgtctgggg gaggccggcg ccgtgttggg
    12421 gcaatcggag tcgaggaagc gtcgcgacat ggcggtcttg ggcggcgggc tggggattct
    12481 gggccttgtg gttgttatca tctggagcgg cctcgtgtcg atcgacgcgc tcgaggtcgc
    12541 gcaggcgacc aaatggttca ttctcgggct ggcggtgctg ttctttgccg gtgtggtgtt
    12601 gttcggtggt ttgagcggtg tcgaaaagaa gcgggtcggt gtcatcgcca tcctctttgt
    12661 ctgctcggcg ttgttctggt gcggtttcga acaggcgggg tcgtcattca acctgttcgc
    12721 gaagcggcat accgaccgcc agtgcctggg atgggtgatg ccggcgggct ggttccaaat
    12781 ggtggggccg gtcttcgtca tcagcctggc gccggtggtc gccgccatct gggtcggcct
    12841 cgcgcggcgc ggcctcgagc catcgcttcc cgcgaagttt gcgttcggac tggcgctgct
    12901 gggggcgggg ttcctggtga tggcgtgggg tgcgaaacgg gctggaaccg gtttggtctg
    12961 gcccacgtgg ctgatctcca catactgcat tcacacgttg gctgaactgt gcgtcagccc
    13021 ggtcggatta agctcggtga cgaagctggc gccggcccgc ttgacgggtc agatgatggg
    13081 cgtgtggttt ctggcgacat ccctgggcaa cctggtggcg gggttgatcg cgggcgaagt
    13141 cagcgatgag agcacccgcc tgatggcgac gcgattcatg caaatcgcgg gcttgaccgg
    13201 cggcctggcg gcggtgttgg ccctgttgac ccgccctgtc cagcggttga tgggaggcgt
    13261 gcgctagccc gcatatttca tggagtcctg ggtcgagatt caacggtctg ccgtgctact
    13321 cttactagga tgagctatgc cattcgatcg tgatcggatg agcgcgcgcg cggtcgagtt
    13381 ggctgcgcgc ggcgtttacg tggggacatc cagttggaag tacgagggct ggatcaacca
    13441 gctctacacc ccggaacggt atgcgtatcg gggacaactg gcacgaagtc gattcgatcg
    13501 cagttgcctt ggcgagtatg cccaggtgtt caagacggtc tgtgtcgacg ccgcctacta
    13561 cgcatttccc ggccgcacga tcttgcagcg aatggctgac caggcgccgg agaacttcct
    13621 tttcggtttc aaggtcaccg acacgatcac ggtcaagaag tttccgaacc tggcgcgatt
    13681 cggcgatcgc gcgggcaagg tcaacgacca gttcctcgac gcggatttgt ttgcgaaggc
    13741 gtttctcgaa ccgtgcgagg cgatccgtcc gaaggtcggg ctgttgatct tcgagttctc
    13801 cggtttctgg ccgacggact acagtcgggg gcgggatttc ctggccgacc ttgacatgtt
    13861 tctgggccgt ctgccgggcg ggtggccgta cggcgtggaa ctgcgcaaca gcaactggct
    13921 gcaccgggac tatttcgagt gtctgagccg gcggcgggtt gcgcatgtgt tcaactcctg
    13981 gggggccatg ccgccggtcg gggagcaact ctccctgccc gagagtcgca cgcatcccga
    14041 gctggtgggc gcccggttcc tcctcaagcc agggcggcgg tacgaggagg cggtgcagtg
    14101 gtttcagcca tacgacaaga tcaaggaggt gaacgaggaa gcccgagtgg cgggggcggc
    14161 gctgatttcg gaaggcgcca ccacaccggg ccgcaagacg ttcatctacg tcaacaaccg
    14221 gctcgaaggc aacgccctcg agacactcga tgcgatggtc ggcctggtgt ctatcccatc
    14281 gactccgctg caacaaggcc cgcggagaaa tcggctgtcg gcgcgctcgt gaaggcggcc
    14341 tgggcctgtc tttaagcgcg cgccgatgcg tggcccggcc tgctcgcggg catcatttct
    14401 tgcgccggtc atttgacctc tgattcagct tctcgtagtc cgctttccat tcatcctcca
    14461 tgtcgcggat gccgggcatg ttttcgcggg catgatcgac ggcctgggcg gcgtctcgcg
    14521 ggctgggaag aacggtgggg cggatcagga cgagcagttc ggagcgtttg gcctctttgg
    14581 atgtgcttcg gaaaaggccg ccaattccgg ggatgtcttt gaggatcggg acgccggact
    14641 tggatttgct cttcgaggcg cggatatagc cgccgaggat gatggtatcg ccgtcctgga
    14701 ccgacacggt ggccgaggcg gaccggtggg tggtattggg catcttgccc acgttggcga
    14761 tctcggtgta accggtgatc tcctcgatat tctgattgat ctccatgacg accagtccgt
    14821 cgggtgtgat gtagggtgtg acatcgaggt aggtctgcac atcgagttgc tgcaactgcg
    14881 cgctggggcc gtagccgtag ccgccgtagc cgccgtagta ggtgctggtc acgtagggca
    14941 ccgactcgcc cacggtgaag ttggccgggg tggcgtggct ggtttggacg cgcggcgtgg
    15001 cgagaatctc gccctttccg ttcgaggcca gggccttcac ggcgacgtcg agatcactgc
    15061 tgtacttgcc gaagtaactg aaaccgcccg gcagcccgct gccgtccatg atgttggtga
    15121 tggcagcgag gaacgacggg ctgtttttcg cggcggcgat ggatgtgaga ttgcccgtct
    15181 gctgctgccg catgaaggtc gagacgccaa actcgaaatt gtcgttgagc tgaacgtcca
    15241 tgataatagc ctcgatcagt acctgggcaa ggaggcggtc gaccttctcg acaatgttgg
    15301 tgatcatctt catgtcgtcc ttggtcgcat agacgatcag cgagttcgat cgctcgtcgg
    15361 ggatgatctt ggcgtccaca accaactggc cttcgcccat tgcggacatc cggctgacca
    15421 ggccctgcag gcgctggttg aaagtgctgg cggcgcccgc ctgcccgagc tgcccggcct
    15481 gcccgacctg agcctgcgtc tggtattggc ctgtctggcg ctgggatccg gtgatgtttg
    15541 tctggcggaa attcgaacga aactggctgc cgccgcgcgc gcctgtggtc ccgcgtcgtg
    15601 tgctgagcgt gccggtcccg cccgcgccca tgccgacgga tgcgccccct cctccgatca
    15661 cgctgctcat ggtcgagtat atctcctcga ctttgccgta cttgattggg atgacttcga
    15721 gcctgtaatc gctttcgacc ttgacgtcga ccttctcgat gatctccatc atccgcttga
    15781 tgttgggagg atagtcgcgg agaacgagcg tcatcgtgct gtcgatcggc accacgccac
    15841 cctgaatctt gccgaatgtt tgaatgacct gcgccacttc ggacggcaat gcattggtaa
    15901 cctgaacaat cttggtcacg aattggccga gttcggccag attcttggcc tcctccttcg
    15961 agaaggccgc gccttcggtc aacgcctgct gcgtcggaac ggcgctgaca aacttgtccc
    16021 ccacatcgat catggtgatg ccattgagcg ccagcacgct ctcgagggcc tggatcgcct
    16081 cttcccggct gagttcctgc tggttcttca gagtcaccga ggtggcggga agtgttgtcg
    16141 gccgcaacac ggtgcgcccg gtcaactccg cgtagacgct caggacctgc tcgatcgggg
    16201 ccacttgcag attgatgact ttttccggga tcatcgacga acccgctaaa cccgccaggt
    16261 tcgtcgtggt cggagtgatc atggccgcca tcggcacgcc ggcaacgctg ggatcgctac
    16321 cccgcggcgc cgcgggctgt gcggaggcgc cgccgggagt cgggattgtt ccggccggca
    16381 aggcggtggc cgccgcggga ccaacaaccc gtggtgtgcc cggaaggccc ccaggccgtg
    16441 cgggaatcgc cgttgcgcca ccccccgccc ccgattccga tgtcaccgtc accgttccgc
    16501 cgggagtctg ggatgaagcg gccggcgtgg gcgctggagt cggcgggagc gggactgttc
    16561 ttggcagttc ccgccggggt ggcggtgtca cgggcagcgt gggtgcgggg gtagccgcgg
    16621 ggccgttggt ttccgcggag ggtggggtct gggcgcgcac accgggtgcg cttccgatca
    16681 aggcgcagca ggcaatgagg cggaaaaacg gaactttcac gtggagcctt tcggggtgga
    16741 gggcgggttg gctgtcggtc cgggggccaa gcgtgtcgcc cccgatctgg gtgtggcggc
    16801 gggcggggcc gccttggcgg tcttctggta gctggcgaca agcgtgatct tgcccagaag
    16861 ccggtagtgg ggcggatccg gtcgcaggtc catgtcccgg acccggatca tggaatcgcc
    16921 ggaaccgagg gcgacgagga agttgacgag ttcctggtca aggcacgaga ggtccacgga
    16981 gacaagctgc tcgtcgaaga actggttggt gctcccggcg ccggcttggg ccgcgggtcg
    17041 gatgccggtg atggcgacgc ccatctcctg ggccttgttc tgcacggtgc gggtgagatc
    17101 gagggcctgc tcggccttga gcaccgccga gcccgccagt tcgagcttct gcaactggga
    17161 gcggtactcg ggcacgcggg cgatctcctt ctgattggtt tgcagcgtgg tccgtgcggc
    17221 ggccttggcg ctctgaagcc ggccccaatc cttgaaacgc ggaaagatga acaacacgct
    17281 gacgacgatc acaatgatcg ttacggtcaa caccacgaac cgctgttcct gcgggcggag
    17341 gcccagtttg tcgaagacgc tcgccatgag tttaaagatc gctgcggcga atctcgcact
    17401 cgatgctcca gttcatcgga cgcgcggtcg agccggacgg gccggcggcc gatgcctgga
    17461 tgctccgggt cgagacttgg ctgaaaagag gttgaccgtt gaccgtggcg gcggccagag
    17521 cctggttgta tgtggtcacc agcccctgtt ggtcggaggg gaccgtgccg aacaggccga
    17581 gcttcttgcc gcgctggaag gtgagctgag tcaggttcaa ctcctgcggg aggttgtcgg
    17641 acgcggcctt ccagcaatcg agcgcggcga acttgaggct gacctgttcc tgcaggatct
    17701 ggactttggc ccggagttcg agggcgttgg tgtaggcgct tgtgctgaag acgatctgtt
    17761 gatccagggc gtccttttga tactgcaccc actgaagcgc cacgaaatag ccgagaatcg
    17821 caaaaagata ggccagaccc agccccccca acgcccgcat ccagagacgg tcgatgaact
    17881 gttgcctgta gcgggacagg aactccgccg gaaccaggtt gcccgcgccc gtcgcggtcg
    17941 ccgacagcgc ggcgagccgg ggcagggcca ggggcggatg gatcgtgagc gcgtctccga
    18001 cggccgtccg cagggcggat tcgagccgcg cggccaggcc ctcctccgca acgaggtgcc
    18061 acgccggatt cggaatcagc caaccctcca tttcgccggc ccaggcgatt tgcctgagca
    18121 ggtcgaccat gtgttgtccc gcctgctcat cgccggaaag ttggagcagg ctcaggttct
    18181 gccactggcc tccgaacctc caggcggcca ggcaaaccgc gcggccgcct tcctcgcggg
    18241 ggagcagcca tacgccgtcg ccgctggcct gaatggcgag cagttcgcgc agttggggca
    18301 actcgaggcg gtcggcgaga aagccgctgt cttcgagccg tccgaggaca tcctcgacga
    18361 cgttgcgcgt ggcgatgatg accacgattg tctgggccgg ggaggccggg ggagagagga
    18421 cctcgaatgt ccagacgacc tgagtgatgg ggagcgggga gagcctttcg atctggagtt
    18481 cgaccatgcc ctcgagttcc ttgggatcgc tggggggcag gtggaccacg cgcaggaaga
    18541 cctggctggc ggggagccag gccacattga gcttcggctg ccagacatcg ctccatgtgc
    18601 gcgccacgtg ccgggccggc agcgggtcgg tcgacagcac aacgcgctcg gagagcagcc
    18661 gggcgccgct gccgtcgcgg ccgaaatgcc agagctggcg gcgctcctgg cgggcatcga
    18721 ggacgttgca gttgttccag cgcccggttt tcacgtgcct tgctagcctt tctggtcctc
    18781 gatgagcgcg gccaggtgct cttcggtttg ggtgacgcgc aggacttcct cgatcgtcgt
    18841 gatgccgttg cgcaccttgc gccagccgtc gacccgcaac gtgcgcatgc cgttctccat
    18901 ggcccgctgt gcgatcgtgg acgatgcggc ccggttgagg atgagcgggc gcagcgcctc
    18961 gttgagaatc agaagctcgt agatgccggt gcgcccctgg tagccaagct gccggcactc
    19021 ctcgcacccg gccccgcgga gaaaacgggt ggtcccgata tgctccggag gaaatgcgat
    19081 ccggtgcaaa tagtcgggtt cgaccgattg ttcggtcttg cagaaaggac agattgtgcg
    19141 gacgagccgc tgggccatga cggcctcgac cgaggacgcc accagaaacg gctcgatgcc
    19201 catgtcgatc aggcgggtga acgcgctggg cgcgtcgttg gtgtgcagcg tgctgaacac
    19261 caggtggccc gtcagcgccg cgcgaatggc aatctccgcc gtctcgagat cccgaatctc
    19321 gccgaccatg accacgttgg gatcctggcg caggatgtgg cgcagcccca tggcgaacgt
    19381 caggccgatg tccggccgga cggcgatctg atttatgccg cgcagctcgt actcgaccgg
    19441 ctcctcgatg gtgatgatgc gtttctgggt cgagttgatc gtgctcagaa acgcgtagag
    19501 cgaggtcgac ttgccagacc cggtcgggcc ggtgaccagg aaaatgccgt ggggcttgac
    19561 gataatctcg cggatggcct cctcttcctc ggggagaaag ccgagcttgt cgagcgtcag
    19621 aaatatcttg ccgcgcgtca gcaaacgcag cgaaacgctc tcgccataca ccgtcggcac
    19681 ggtcgacacc cggatgtcga tttcctcgcc tttgatgcgc acgttgatgc gtccgtcctg
    19741 cgggaggcgt ttctcggcga tgttcattcc cgacatcacc ttgatgcgcg agatgatggc
    19801 cgcctggaac cgcttcaact ggggctgcat cggcgtctgg tgcaaaatgc cgtcgatccg
    19861 gtagcggatt cgcagctcat cctccatcgg ttcgaggtgg atgtccgtcg agcggtcctt
    19921 gtacgcctcc cagatgacct ggttgacaaa cttgatgacg ctcgcctcct gatcgccctc
    19981 cgtgatctcc cggtcgctcg caatctcgag ctccaccgac tcctcttccg acatctcgtc
    20041 cagagtctcg gccccgaccc cgtagtactt cttgagcgcc ttctcgatct ccgcttttga
    20101 cgccagcgcg aattcgagcg gcgattgcgc ctcgaactga accgcattca ccatggccgg
    20161 atcgaacgga ttggccaccg ccacctggag acgatcctcg atcaggcgca tcggcatcac
    20221 ggtgaactgg aacgccacct tcgtcgatat cttcgagaga atcagcttcc gctcctcgcg
    20281 gggcacatcc tccggaatcg tcacgcggtc gacatacgtg tgccccagcg cgcccgccaa
    20341 cgcccggaga aacgcctcct ccgacaaacc gctctcgcgc tggaagaccg taagcagcga
    20401 atcctgcccg ccgctttcga cctgcgcgcg ccacgtcttg tgccattgct cgcactgctc
    20461 gggtgtgacg agcgcggcct gggccagaac ggtcttgatg gacgctaatg ccatgggaca
    20521 catcggatcg ccgatgacct atggctatac aaccgaaacc ggcaaaagtt gcgacctccc
    20581 atgattttcc gccctcagcc ccgccgcgtc gaaaagccga cccacccgcg gttcgcccgg
    20641 ccgaggccgg tttcggtctc agcttccaat caactccttt gccgcgcgga ccatggcgtt
    20701 aaccgtcagg ccgtgcttct cgtagagctg atccgccagg taggcggact ggccgaactc
    20761 gccccgaatc cccaacgaaa tcatccgatg agcaaccttc gcgttggcca gagcatgggc
    20821 gacctgcgcg cccatcccgc ccatgatctg gtgatcctcg atcgtcacca cgcgcccatg
    20881 acaggcgctc accgccccgc cgatcgtgtc gaggtcgacc cggttgacga acgggttgtt
    20941 gatcacggtc gcccggaccc cttcggaagc cagttgccgc ccggcctcga tggccttgcc
    21001 caccagcgcc ccgcatccga tcaacaccac atccccgccc tcctgaagca cctgcgccct
    21061 gccccactcg aattgcgccc ccccgaccca cgacgtcaga tagctctccc gccctatgaa
    21121 gaagatcacc gattcgccgt cgtcgccgcg ccttcgggcc tcgccgagcc tctggatggc
    21181 ctgggccatg aatgcctcgg cttcatccgc gcacgacgcg gcgatcacca ccgtgtgcgg
    21241 aatggcgctg gtggcggcaa gatacgtggt ggcctggtgc gaagccccat ccgccgcgtc
    21301 ttgcagaccg gcgtgcgaga agactgcgat cacgggcgcc tgggagagcg ccgccatcgt
    21361 caacggcaga ttgcccttcg tgactccgaa ctgggcgaac gtgtcgacaa tcggaatgaa
    21421 ccccgacttg gacaggccgg ccgcgacgct gatcatgttc gcctcggcga ccccgacatc
    21481 gacgaaacgg tcgggaaatg tcttctgaaa gagactcatg cccgtcgatc cctgaacgtc
    21541 cgaggagacc gagaaaacag gccatccctc ccgggccgcc cggattgcgc ctttggccag
    21601 gccggactgg accttgtctt tcttgggtgg agtggtggac tgcagggcgg ccgcggcggc
    21661 cttttccttc ttcgccgctt ctttcgcctc ccagtcggcg cgcagttcga gggcccagcg
    21721 gagcaaggcc tccggcggtt ccccgccgta gatttctcgg acaaactcga cgatcttctc
    21781 gccgttcgac agcgggaacc cgtgtccgcc cgaggcgctc tgctccgtgg ccttgacgcc
    21841 gtatcccttg atggtcttgg cccagacaca cgacggttga cgcggattgg ccctggcctg
    21901 ggcgacagcc gattcgatgg ccccgtagac ggccgccagg tcgtggccat ggtccaccac
    21961 gatcacgcgc cacccaagca cctgcatcgc ctcgaacgtc ggccgcatgg cgaatgcgtc
    22021 cgccgcgatg cgcccggaca acttggcatt gttgtccgac accaccatca cgaatggatt
    22081 cagcaggcct ttcgaggcca tgccgggaat cgcggcaaag gcctctttgg cctcgccctc
    22141 catcgccgcc ccgtcggaca ccgccgcgat ggtcacccgc tccgcgccag ccagccgatc
    22201 tgccaaagcc agtccctggg cctgcggcaa agccgatccc agcgggccgt tgctcagcag
    22261 aacgccctcc ggattcaaat gcgactcgcc atggccggtc agcttgctct cgagactccg
    22321 aaaagccctg agcgattcga acgtcagccc gtcgtaccca tagttggctc ggagggcgta
    22381 aagaccgttt tcagcgtggc ccgcgtcgtt gacgaaattg taggcctcgt gccacgggcg
    22441 gccctgagtc gagaacatca ggccatgaat ggccgccatg atctccgcaa aggcggcggg
    22501 cccgccccag tggcacgctg ctcccccgtt gaccgcatgc acgttcatca gcgcaaccag
    22561 agcgcgcgtg gcgcgcgggt cggccaggac aacctcgtgc ccggcggcgt cgcggatggt
    22621 gacggcatac cggggcttgc ccggcggcgt gggggcgagc ttggcaggcc ggctcagcgg
    22681 acgcagcggc gatgcctccg ccggctgcac tacagtcttg tcttcaccca taaaaactcc
    22741 agatgcgccc cggtctcgag gcaacatcgg cagcctagga aacgactgga aaaagaaaaa
    22801 gagaaaaaca aacagccctt gcaggcatct ggagattgtc ggtggggagc gagtttgcgc
    22861 cgcgtggggc ctgcctgccg cgcctgcctg tgccgtgccc gacacggcag acaggccgct
    22921 gcgctttgcg gcgcaggcag ggcacgcggc ctacaccggg agtctgcaac ttaacttttg
    22981 cacagaccgt tagcccgatt cgatccgata gcgcagcaga aactcgctgc ggtcggcgcc
    23041 tccgaccccg cgtccgagaa tccgcaaggc atggcggcct gctcgaaact ggccgtgcgg
    23101 actctcgcgc ggcagcggtt cgagggccca cggcaacggg tcgtcgtaat tcaacgggct
    23161 gttcccccag tccacctcga ctccgtggaa ccagaggtct ccgtccagat tcggatcgag
    23221 ccgcaagggt gctttcgaga ggggcctg
//
LOCUS       DAOQRO010000004        22620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1514_length_22620_cov_5.591004, whole genome shotgun sequence.
ACCESSION   DAOQRO010000004 DAOQRO010000000
VERSION     DAOQRO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 22620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22620
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_22620_cov_5.591004"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKK20_00330"
     CDS             complement(<1..1202)
                     /locus_tag="PKK20_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98369.1"
                     /translation="MATTPLLPLSDLINSEFVPVEILFDADGSFSLTYNGKVHFSKFF
                     FPGYEPLSGARIGFGARTGGLNAHHWIDDLSIVTYTDPKPGIVRQPENLTMVETSAAK
                     FTVTLNNGDGATVQWLRNGTAIGGATGTSYTLSPVSMTDNGAKFSARVTLGANIVTSD
                     EATLTVVRIDLPDAPVVSYDFNDGLVPVEAMVYGLGFAIDGSEWAPYVSPNMGVGNSG
                     VLLITENINSQSGAFIIPDPHAAAPVYGVAARFDVLIGGGTEVPADGMSFNFANDLPY
                     GTAVGNAEEGQGSGLRVCFDIYDNTDGNPNNGVGEAPAITLKWGETVVSEVKTALSDI
                     TTGDQYADVLVRLTPDGLFDVAWNGKVLMYRVPVPGFGSITEGRFGFFARTGGLNANH
                     WIDNLRIYT"
     gene            1311..2108
                     /locus_tag="PKK20_00335"
     CDS             1311..2108
                     /locus_tag="PKK20_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNU98370.1"
                     /translation="MKRPMRWMRPRALLVGCAVFWIAGCVTAPETGRRQLVLIGSAQE
                     MQLGLQAFSDMKKETPVSKNAAAMALVEKVGRRIAAVADLPGAEWEFVLFESPEANAF
                     CLPGGKVGVYTGILPITKDEAGLATVIAHEVAHAAAHHGAERMSRAIITQGLGEVASA
                     YVGGKDPRYQAAFGSLYGIGVQVGETLPHSRKQESEADQIGLIYMAKAGYDPEAAVAF
                     WERFAEYNRRQGSQTPAFLRTHPLDERRIADLKQWMPQAKAAYRPAR"
     gene            complement(2059..3156)
                     /locus_tag="PKK20_00340"
     CDS             complement(2059..3156)
                     /locus_tag="PKK20_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNU98371.1"
                     /translation="MIDTPAMLTGFVARLRAADWVAMDTEADSLHSYPEKVCLMQFSM
                     PGEDALVDPLSGMDLAPLWQALEGRTLLLHGADYDLRMLYRDYQFIPHAVFDTMLAAR
                     VAGMREFGLSALVESLLGTKLDKGPQKANWSRRPLTDRMIVYARNDTRYLHSLATLLA
                     DRLRALDRLDWHRQMCAQLVHDCTRATEGDPDGLWRIRGSSRLEPKALGILREVWMWR
                     DREARAANQPPFFILNHDHLLALAEQAARGESVDALIPKRFSLRRRRALFEALDRGAR
                     LRKASRPLPLRPRGHRLTHAQRLRLERLEARRNRHAATLNLDPSLIASRSDLVALASG
                     CERQERALLPWQKRLLIEPDDRPLSPGASTA"
     gene            3287..4759
                     /locus_tag="PKK20_00345"
     CDS             3287..4759
                     /locus_tag="PKK20_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNU98372.1"
                     /translation="MNLTTAFLQSAERNATKAAVLWGDAERSYRSFADQSAWVTHHLR
                     TRLGVKPGDRVGLWLRNCPEFISAFFGILGAGAIVVPINNFLKANEVGYILRDGGIDV
                     LVTEGGMEETLERLTAARPGLGLLRTETFEAAPAHPVELPGLGQEDQDLAVIIYTSGT
                     TGHPKGAMLSHGNLLHNVESCRHVFETVEVDRFALVLPMFHSFMLMVCVLLPLIVGGT
                     IVLVKSLNPPKNIVREIMQHRATVLPAIPQLFRALAHIPLPPDFPLRLCVSGAAPLPL
                     EVLREFSRQHTIPLVEGYGLSEASPVVSVNPIRGIQKAGSIGLPILGVEISIRNGENK
                     ELPRGETGEVCVRGGNVMLGYWGKPEETDSALRESWLHTGDVGYMDSDGYTYITDRKK
                     DMLLVNGINVYPREIEEVIYQYPGVREAAVIGKPDSRKGEHPVAFVSMNDGVVIDELA
                     LFHFLKDRLADYKLPRKVFQLEMLPRNATGKILKTALREM"
     gene            5012..5152
                     /locus_tag="PKK20_00350"
                     /pseudo
     CDS             5012..5152
                     /locus_tag="PKK20_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301710.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
     gene            5258..6433
                     /locus_tag="PKK20_00355"
     CDS             5258..6433
                     /locus_tag="PKK20_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNU98373.1"
                     /translation="MPQQLAYAIITPHSLHKSRTGGIIARLITRSGVELVGARMFAPS
                     AELVQKYSESIVSAQDGQDRRVQELIRDYVIENFAPEPKSGRRRRVMMLLFQGEDAVR
                     RVRTVVGNFSPHRRGGQTIRDTYGDLVLDANDEVRYFEPAVLAAPSLDEAIAKLKLWA
                     RYSDTEGGVLDQVISYAADEQSERTLVLLKPDNFKFATGRPGNMIDFFSRTGLFIVGI
                     KVHRMSTAQAMEFYGPVREILRTKLKSVVATRAKEVLEKELGFAIGASESQQLGELLG
                     PLLGENQFENIVRFMAGRSPSECEPAQMTQPGTEKCIALVYEGVNAVAKIRDVLGPTD
                     PTKAPPGSIRREFGSNIMINAAHASDSAENAKREMRIVNVAENNFRQIIEGFYGRVS"
     gene            6525..8105
                     /locus_tag="PKK20_00360"
     CDS             6525..8105
                     /locus_tag="PKK20_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU98374.1"
                     /translation="MNAAATPPNHDGPPWWRLPNRYQWYVFALAAFGWLFDCFDQQIF
                     TLSRSITMRDLLPHADTLTQTTRGGYATSIFILGWATGGLFFGMLGDRWGRAKTMATT
                     ILVYAACTGLSGLSQSWGYFALFRFLAGAGVGGEFAVGAALVAEVMPDRARPHTLGSL
                     QALSAIGNVLAAVSLGVVVPDERLGWGWRGLYFLGALPGLVAVVVFLRLKEPEKWVAA
                     RAAAARGGSSRQFGSIKELFRIPVWRRNTLVGVSLAVAGVLGLWGVGFYSPELIDSAI
                     TTVETETRPKIEAVLRADTPAAHSAALAGLSAQENRKYLELLSRIGAADTRSTSTDPA
                     ASPLTGDQKERLAQLLAGAITEDRKTHLKSVGGILQQVGSFLGITFFTAIAARLGRRL
                     SFLIGLTIAWASLILVFGTFHQAHQIYYLWPILGFCTLAPFGGYAIYFPELFPTRLRT
                     TGMSFCYNVGRYIAALGPFVLGPLARALDGLTAIPGFRLAAMTLACSYLLGIVALIWA
                     PETLGRPLPDDERENTGA"
     gene            8246..8599
                     /gene="raiA"
                     /locus_tag="PKK20_00365"
     CDS             8246..8599
                     /gene="raiA"
                     /locus_tag="PKK20_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNU98375.1"
                     /translation="MKLILTTHNVTLTSAIEEHILEKLDKLEHFNRWAIDARVTLDHD
                     HTKAPKKQFGCSVRLGVRGPDLFAEDSQNDLYTAIDMVFKKIEQQIRKRRGKRETVKH
                     THAARLKRKRQDTEL"
     gene            8701..10017
                     /locus_tag="PKK20_00370"
     CDS             8701..10017
                     /locus_tag="PKK20_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNU98376.1"
                     /translation="MAVESLKVLRARVVLPIRQPRLPNAAVVVSGGRIQAVGQWKDLR
                     PHCAGSPTDLGEVALLPGLVNAHCHLDYTDMAGQLAPSRSFTDWIKRITELKGAWNRD
                     DFARSWWHGAQMLIRTGTTVVGDVESAPDLLPEMWQATPLRLTSFIELTGVKSRRTPR
                     AILDEALAAIAALPRGRHRAFLSPHAPYSTPPELLRRCGAITRRRRWRITTHVAESEE
                     EYAMFTRRGGPMFDWLERNQRDMSDCGGISPIQHLARQNLLGDRLLAVHANYLDRGDA
                     DLLAINRVHVIHCPRSHAYFGHRPFPVTALMRRGVNVCLGTDSLATVATPRGQTAALD
                     LFEEMRVFAQAHPRWPPDAVLRLATLNGARALGFAGRVGEITPGAWADLIAIPSSVGL
                     REVAAALVHNRQPVVAAMIGGEWIAGFPGAYCTPSVQSAVGLREAL"
     gene            complement(10027..10446)
                     /locus_tag="PKK20_00375"
     CDS             complement(10027..10446)
                     /locus_tag="PKK20_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNU98377.1"
                     /translation="MNLLVFFAALFVVVEALNATGLPDQIYGHVRGLFGHTLAAQGWN
                     LAWFSALGSNIFSNVPFVLVAGKWISNFVEPKRMWQVIALATTFSGDLTILGSVANII
                     VVESARKHVEVGFWDYAKFGLPVTVLTLVAGMRILLR"
     gene            10671..11867
                     /locus_tag="PKK20_00380"
     CDS             10671..11867
                     /locus_tag="PKK20_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU98378.1"
                     /translation="MSKAESKMDSRERVRCTMRHLLPDRVPVFCQLALGHYFLHSGIA
                     PHEIWFTTEGFAQALVRLQQRYRFDGILVNLPGRPPDLLSRVQQIDATDEGTRLRWAD
                     GSCTTVPPDDNPQHRLATQASLPRADFARLDPDRLDALDALTGYWWNVCHVPWIDGKT
                     SPGLLRRIPGYFGDTIDRVRALTRGAVSIHGEVFSPFTHFMELFGYEEALMALVTDPG
                     KARAFLDRLTESSIAWGVAQARRGADAVLVSSAFAGAGFLSKAAYAGFVLPYERRVAR
                     AIRAAGVPVYTHTCGRIGDRLDLMIETETQGLDTLDPPPLGDVDLAAAKRQLEGRLFI
                     KGNMNSVALLAYRAPGEVEAEVRRCLRDGAPGGGYILSTACSVAPRVEPWKLELIAAL
                     AEKERY"
     gene            complement(12117..12977)
                     /locus_tag="PKK20_00385"
     CDS             complement(12117..12977)
                     /locus_tag="PKK20_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNU98379.1"
                     /translation="MIIVTDSRCTAYQQFGHPERPQRISLTVERLRAQSEIELVWAEP
                     APVTEAQLERAHKRSLLERLKVSVDFDSDTAAYPDIRAHAERSAGGALYALQRALESK
                     TAFSLLRPPGHHATPDRAMGFCYLNSAAIAVMEARAKGVGRVAVYDFDVHHGNGTEDI
                     LLGQDQCAYFSVHQFPCYPGTGSDSYGNAHNYPVAPGAAREDYRQALAKALEDLERFK
                     PELVVVSAGFDAYCGDPISDAALQEEDFGWLGCSIRELGVPAFSLLEGGYSRDLPDLI
                     FAYLRGLAGK"
     gene            complement(13106..14680)
                     /locus_tag="PKK20_00390"
     CDS             complement(13106..14680)
                     /locus_tag="PKK20_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98380.1"
                     /translation="MTLFPIVQRELLVASRRPGAFWARLVAAAVAVAVGAINLWYHGE
                     WTGGTRIGRELFDTLAFVGYLFCLAAGPLFTADVLSEERRDGSLGLLFLTDLRSYDIV
                     GGKLVAASVTALFSLMAALPVLAIPILLGGVRLIEFGAVALVLGNALFFSLAVGMCVS
                     AASTQARSAFALAVFILFLFAGVGPWLRLVIGSYTGLGILLAANPSSALELASSVAAN
                     REGVVLGWALSGTHGLAWLFLGSAAWLTQRAWREQGVLKAGVPWRHRWHMLNYGTAIE
                     RQHSRRRLLDRNPVLWLASRNQLKVRLLWGAVGLALFVWLVWRWRMGMASDTWNSTFL
                     IALFLHAPLKWLMASEASQRWTQERQTGALELLLTTPLSVAEILRGHWLSLRRLFGAP
                     ALAILLVEVFTLAASEGLNGLGSDMGLMTLVGALVFIWDLYALAWVGLWLGLSSRKAN
                     QAFVGTAVRILCVPWAVFLFALFFTGVQGPLALPLLWLIVCGLCNLTFQRWARENLRG
                     LLRELAPGRLGHAAAN"
     gene            complement(14759..16507)
                     /locus_tag="PKK20_00395"
     CDS             complement(14759..16507)
                     /locus_tag="PKK20_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU98381.1"
                     /translation="MWTLFPVVDRELRTAARRSKTHWVRLAAATAAFVVSAWIALWVA
                     KTQTPVTAGRWLFSCLTILAFGYCLLVGPFITADSISEEKREGTLGLLFLTELRSYDV
                     VLGKWLANSLAGFYGLLAILPALGIPLLMGGVTAGEYVRTALAVVNAILFSLTAGMFV
                     STLSRDQSKAILGSAVAILGVSGLIPGLAAVFSSGLFTRPLPSPLPAALMSPAYTGYL
                     ASERAYATAPGAFWVSLGFVCGMSCLCMGLTAIWLPRLWRESPEEKPIAHRWLWRLGY
                     TAGWRATYKRRLETNPVYAVAARLRWPHWVFWALVSLVAVNVYWLTHGYRTNTGASAF
                     HTYFSHGLVFTNRVWLCVMACRFWLEARRTGALELILTSPMPARTLLRGHWRALWLLF
                     AGPVAMIALLHIFYIGENWRLAAQRGPFSHGLLQQYAFSAGSSLLNFVTDVFAICWLG
                     AWLSLALRRPTFAILKTFAWVVLGPWTLGVVFPNLSSLLPVKVMTYLNTSTILRTLGG
                     GMVGYSMVFRTLGWVGKNVLIILWARGRLRRHLRSAAAQTHEWEFGARFRRRRSRPHV
                     SWTVPHAVAGESRILS"
     gene            16657..16881
                     /locus_tag="PKK20_00400"
     CDS             16657..16881
                     /locus_tag="PKK20_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HNU98382.1"
                     /translation="MYAAVMTAIDESLRNKSTLYQDFLAERDEILRHKWIESEKAGYD
                     IGFERALTDWSVHHRGPWRRWHQSRRAGLS"
     gene            17026..17580
                     /gene="pyrR"
                     /locus_tag="PKK20_00405"
     CDS             17026..17580
                     /gene="pyrR"
                     /locus_tag="PKK20_00405"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNU98383.1"
                     /translation="MPTRSHKNRVVMDSRAIDLAVRRVARAIASRAEGHARVVLVGIQ
                     TGGVWLARHLAQHLERLWSAPVPVGQLDISMHRDDLDQRLAPQVHPTAIGFDVTGKTV
                     ILVDDVLFSGRTIRAAMDALTDLGRPRRIQLAVLIDRGHRELPIEADFVGKRIATGPT
                     DRVDVRLADTLAASQVVLEKAVRR"
     gene            17593..18549
                     /locus_tag="PKK20_00410"
     CDS             17593..18549
                     /locus_tag="PKK20_00410"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNU98384.1"
                     /translation="MLWTRKDLLDLQSLSVEEITAVLDTARAFRAVGERTIKKVPALR
                     GKTVINLFIEPSTRTRISFELAAQRLSADVINFTAEASSLKKGETLKDTARNLEALNA
                     DFIIIRHSAAGAPHFLARVLNAHVVNAGDGAHEHPTQGLLDIFTMREKKGSLQGLRVT
                     ILGDILYSRVARSNIWALRKMGAHITLCGPPTLVPRIFEQMECRVTSDLEEAIADADV
                     INLLRIQHERQRKTMFPSLAEYSSLFGINRARLAATKPDVLIMHPGPINRGVEVDSEV
                     ADGERSVILEQVTNGLAIRMAALYLVNGGQAASTETPEQAAA"
     gene            complement(18622..20568)
                     /gene="thiC"
                     /locus_tag="PKK20_00415"
     CDS             complement(18622..20568)
                     /gene="thiC"
                     /locus_tag="PKK20_00415"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNU98385.1"
                     /translation="MIATEDRSKSIRADSLPGSRRVYLPGRIHASVRVPMREIALSPT
                     RLANGRIRPNAPVRVYDCSGPWGDPDFHGDVSQGLPPLRREWILARGDVEEYESGGQG
                     WSSANLDRAGAPEVSAPPGGPSHFAPRRPLRAKPNQRPTQLEYARRGVITPEMEFVAI
                     RENLGFDRLEAERARDAARNDLRHQHAGPTSVDPTAAPSVFSRFRPRVPRKITPEFVR
                     DEVAAGRAIIPANVNHPESEPMVIGRSFLVKINANLGNSAVASSIEEEVEKMRWAIRW
                     GADTVMDLSTGPNIHATREWILRNAPVPVGTVPIYQALEKAGGRAENLTWEIYRDTLI
                     EQCEQGVDYFTVHAGVLLRFIPLTVHRAAGIVSRGGSIMAKWCLAHHRENFLYTHWDD
                     LCEILAAYDAALSIGDGLRPGAIADANDEAQFAELETQGDLTRRAWDRGCQVMNEGPG
                     HVPMHLIEENMHRQLEWCHEAPFYTLGPLTTDIAPGYDHLSSAIGAAMIGWYGTAMLC
                     YVTPKEHLGLPNRKDVKDGVIAYKIAAHAADLAKGHPGAQYRDNALSQARFEFRWEDQ
                     FNLSLDPETARAFHDETLPQEDAKTVHFCSMCGPRFCAMKISEDVRRSAAEQDVTERQ
                     AIQSDMDRKAREFVDKGAELYGTT"
     regulatory      complement(20643..20770)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20896..>22620
                     /locus_tag="PKK20_00420"
     CDS             20896..>22620
                     /locus_tag="PKK20_00420"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HNU98386.1"
                     /translation="MPSPHMNTPARPDPLTSIAGTAPSSPNPTVSPFLCLALLALSLE
                     ASLPALGAPEGATVREYRKTFRTYPFSDPDPIPNGGQIYPYFRYDGFTDRAEDREWTV
                     VELENPWIRVMVLPEIGGKVWSAVEKSTGKEFLYGNQVVKFRDIAMRGPWTSGGIEAN
                     YGIIGHTPNCATPVDYRTRRKADGSVSVVIGVLDLLTRTPWRIEIALPADKAYFTTAS
                     LWHNATPFEQPYYTWMNAGIKAAGNLQFVFPGTHHIDHDGRAQPWPIDPKTGRDLSFY
                     ERNDFGSYKAYHVLGRGADFFAAFWHDEDFGMGRFSYRDEKLGQKVWIWGLSREGMIW
                     EKLLTDTEGQYVEVQSGRSFNQAGGGSSRTPFKHRGFLPYATDTWTEYWFPVKGTKGL
                     VAASPFGGLNVRPREDGLEVLFSPLQAVDDTLEVLDGERIVFSSLVSAKPLEPWTAIV
                     PVTAPVERLRVRIGGNRLEWKGDPGAGELSRPLQSPPDFDWTSAYGLWLKGKELLRQR
                     SYAPAREALEASLRKEPHFVPALADLSLLRLFAMDAKGAFDLARKALAVDTYDPAANY
                     CYGLAARRL"
BASE COUNT         4247 a         7546 c         6946 g         3881 t
ORIGIN      
        1 gtgtagatgc ggaggttgtc gatccaatgg tttgcgttga gtccgccggt gcgggcaaag
       61 aaaccgaacc ggccctcggt gatcgagccg aatccgggga ccggcacccg gtacatgagc
      121 accttgccgt tccaggcgac atcgaacagg ccgtcgggcg tcaggcgcac gaggacgtca
      181 gcgtattggt cgccggtggt gatgtcgctc aaagcggtct tcacctcgct cacaaccgtc
      241 tcgccccact tgagagtgat ggcgggggct tcgccgacgc cattgttcgg gttgccgtcg
      301 gtgttgtcgt agatgtcgaa acagacgcgc aggcccgagc cttgcccttc ctcggcattg
      361 ccgacggcgg tcccgtacgg aaggtcattg gcgaagttga agctcatgcc atccgcaggc
      421 acttcggtcc ctccgccgat cagcacgtca aatcgggcgg ccacgccata gacgggggca
      481 gcggcgtgcg ggtccggaat gatgaaagcc ccgctctggc tgttgatgtt ctcggtgatc
      541 aggaggacgc ccgagttgcc gacgcccatg tttggactga cataaggcgc ccattcgctt
      601 ccgtcgatgg caaagcccag gccatacacc atggcttcca ccggcaccag gccgtcgttg
      661 aagtcgtacg acacgaccgg cgcatccggg agatcgatcc ggacgacggt cagcgtggct
      721 tcgtccgaag tgacgatgtt cgccccgaga gtgacgcgcg ccgagaactt cgcgccgtta
      781 tcggtcatcg agaccggact caacgtgtag ctcgtcccgg tagcgccccc aatggcggtg
      841 ccattgcgca gccactgaac cgtcgcgccg tctccattgt tcagcgtgac cgtgaatttc
      901 gcggcgctcg tctccaccat ggtcaggttc tccggttgcc ggacaatgcc gggtttggga
      961 tcggtatagg tcacgatgct cagatcgtcg atccagtggt gggcattcaa acccccggtg
     1021 cgcgcgccga atccaatccg cgcccccgac aaaggctcgt agccgggaaa gaagaacttc
     1081 gagaaatgaa ccttgccgtt gtaggtcagg ctgaagctgc cgtcggcgtc gaagagaatc
     1141 tcgactggca cgaactcgct gttgatcaga tcgctcagtg gcagcagcgg cgttgtggcc
     1201 acagtcggtt tgaggtttgt aggctgaggc gctacatatg cacctcccca tcggccttgt
     1261 caagcggacg cgacttgagt ctgctgcccg tttgtgcttt cgtcaggccg atgaaaagac
     1321 cgatgcgatg gatgcgcccg cgggcgctgc ttgtgggatg tgccgtgttt tggattgccg
     1381 ggtgcgtgac tgcgcctgaa accgggcgca ggcaattggt gctgatcggg agcgcccagg
     1441 aaatgcagtt gggtctgcag gcgttttcgg acatgaaaaa ggagacgccg gtgagcaaaa
     1501 acgccgcggc gatggccttg gtcgagaaag tcggacgccg gatcgcggcg gtggcggacc
     1561 tcccgggagc ggaatgggag ttcgtgctgt tcgagagtcc ggaggccaat gccttctgcc
     1621 tgccgggcgg caaggtcgga gtctacacgg gcatcctgcc gattacgaag gacgaggccg
     1681 gcctggcaac ggtgatcgcg cacgaggtgg cgcatgcggc ggcccatcat ggagccgagc
     1741 ggatgagccg ggcgatcatc acacaaggcc tgggcgaggt cgcgtccgct tacgtgggcg
     1801 gcaaagaccc gcgctaccag gcggcgttcg gctcgctcta cggcatcgga gtgcaggtgg
     1861 gcgagaccct gccgcacagc cgcaagcagg aatccgaggc tgaccagatc ggtctgatct
     1921 acatggccaa ggcgggctat gatccggagg cggcagtcgc gttttgggag cggtttgccg
     1981 agtacaaccg ccggcagggc agccagacgc cggcgtttct gcgcacgcat ccgctcgacg
     2041 aacggcgcat tgccgacctc aagcagtgga tgccccaggc gaaagcggcc tatcgtccgg
     2101 ctcgatgagc aaacgcttct gccacgggag aagcgcgcgt tcctgccgct cgcaaccgct
     2161 ggccagagcc accaagtccg agcggctggc gatcaggctc ggatcgagat tcaacgtcgc
     2221 ggcgtgacgg ttgcgacggg cctcgagtcg ttccaggcgc agtctctggg catgggtcaa
     2281 ccggtgtccg cgcgggcgca acggaagggg ccggctggcc tttctcaaac gcgcaccccg
     2341 gtccaacgcc tcgaagaggg ccctccggcg ccgcagggag aaacgcttgg gaatcaaggc
     2401 gtcgacactc tcgccccgag ccgcctgttc ggccagagcg agcaggtgat cgtggttcag
     2461 gatgaagaaa gggggctgat tggcggctcg ggcctcccgg tcccgccaca tccagacctc
     2521 acggaggatt cccaaggcct tcggttcgag ccggctcgat ccgcggatgc gccagagtcc
     2581 gtcagggtct ccctctgtcg cccgggtgca atcgtggacc agttgcgcgc acatttgacg
     2641 gtgccaatcg agccgatcca gcgcccgcaa acgatcggcc agaagcgtgg ccaaggagtg
     2701 cagataacgc gtgtcgttgc gggcgtacac gatcatccgg tccgtgagag ggcgccgaga
     2761 ccagttcgcc ttttgcgggc ctttgtcgag cttggttccc aggagcgact cgacgagcgc
     2821 gctgagcccg aactcgcgca tgccggccac gcgcgcagcc agcatcgtgt cgaaaaccgc
     2881 gtgcggaatg aactggtagt cccgatagag catccgcaga tcgtaatcgg cgccatgcag
     2941 aagcagcgtt cgcccctcga gggcctgcca gagaggggcc aggtccatgc cgctgagggg
     3001 atccacaagc gcatcctcgc cgggcatgct gaattgcatg agacagactt tctccggata
     3061 cgagtgaagg ctgtcagcct ccgtgtccat ggccacccag tccgccgcgc ggagtcgggc
     3121 gacaaaacca gtcagcattg cgggtgtgtc gatcaccgcc tcagtaaggg acgaagagca
     3181 cgaaaaggga agccggatgt tcggcccagc gatcgagaag cattcacttg actgatgggg
     3241 ccgcagaaca ttaagtctcg gcctaacagc tcggaatcac tagccaatga acctgacaac
     3301 ggcctttttg cagtcggcgg agcggaatgc gacgaaagca gcggttctgt ggggcgacgc
     3361 cgagcgaagc taccgatctt ttgcggatca atccgcctgg gtcacccacc atttgcggac
     3421 ccggttgggc gtgaagcctg gggacagggt cggcttgtgg ttgaggaact gtccggagtt
     3481 tatctcggct tttttcggca ttctcggcgc cggggccatt gtcgtcccca tcaacaactt
     3541 tctcaaggcg aacgaggtgg gttacattct gagggatggc ggcattgatg tgctggtgac
     3601 tgaagggggg atggaagaaa cgctcgaacg gttgacggcg gcgcgtccgg gccttgggct
     3661 gttgcggaca gagacgttcg aagcggcgcc ggcgcatccg gtcgaactgc ccggactcgg
     3721 acaggaggat caggatctgg cggtgatcat ttacacgtcg ggaacgacgg ggcatccgaa
     3781 aggagcgatg ctttcgcacg ggaacctgct ccacaacgtc gagagctgcc ggcacgtctt
     3841 cgagacggtc gaggtggatc ggtttgcgct ggtcctgccg atgttccaca gcttcatgct
     3901 gatggtgtgc gtcctgctgc cgttgatcgt gggcgggacc atcgtgctgg tcaaatcgct
     3961 caacccgccc aagaacatcg tgcgcgagat catgcagcat cgagccacgg tccttccggc
     4021 catcccgcaa ctcttccggg ctctggcaca catccccctg ccgcctgact tcccgctgcg
     4081 actctgcgtc agcggggcgg cgccgctccc cctcgaggtc ctgcgcgagt tttcccgcca
     4141 acacaccatc ccgctcgtcg aaggctacgg actgagtgag gccagcccgg tcgtgtcggt
     4201 gaaccccatc cgcggcatcc agaaggcggg atcgatcgga ctcccgattc tcggcgtcga
     4261 gatcagcatc cggaacgggg agaacaagga gttgccccgc ggggagaccg gcgaggtctg
     4321 cgtgcgcggc gggaacgtca tgctggggta ttggggcaaa ccggaggaaa ccgacagcgc
     4381 tctccgagaa agctggttgc acacggggga tgtgggttac atggactcgg atgggtacac
     4441 gtacatcacc gaccgaaaga aggacatgct cttggtcaac gggataaacg tctatccgcg
     4501 tgaaatcgag gaagtgattt accagtatcc cggggtgagg gaggccgcag tgattggtaa
     4561 gcccgattcg cgcaagggtg aacatccagt ggcgtttgtg tcgatgaacg acggggtcgt
     4621 gatcgacgag ctggctctgt tccactttct caaggaccgg cttgcagact acaagctgcc
     4681 tcggaaagtc tttcaattgg aaatgcttcc gcgcaatgca acaggcaaga ttctcaagac
     4741 tgccctccgc gaaatgtgat cgcgacaaga ccctcttgga gccgacccgt ctggcccgca
     4801 tatttgcact ccgatggtgg accgggcact cggacttcgc tagaggcctg ttgcgctgga
     4861 taagtcgttc cctaaaagca agatacacac gatccctcgc ggcgctgagt ccggtcgtgt
     4921 gagctttcgc agttgacgct ctggtttcct gccctttaag ctctatcccg ttacagcagc
     4981 agtgcgtgac gcattgctgc tggagctttc tatggccgaa ggttactgcg taaaatgcaa
     5041 agctaagaag gagatcgccg acggggtcga ggaagtgatg aagaacggac gaaaggcgat
     5101 caaaggcaaa tgtcccacct gcggggtggt catgttcaag attctgggcg gcaaggcggc
     5161 cgccccgccc gcggcggccg aagcagtacc cgacaccgcc ccgagtggaa ccggcggggc
     5221 cgaaggtgcg ccaccgacac tgtagattca ctgttttatg ccccagcaac tggcttacgc
     5281 catcattacc cctcactccc tgcacaaatc gcggaccggt ggcatcatcg cccggttgat
     5341 cacccgctcc ggtgtcgagc tggtcggggc gcgcatgttc gctcccagcg cagaactggt
     5401 tcagaagtac agtgagtcaa tcgtctcggc gcaggatggc caggatcgac gcgtgcagga
     5461 gttgatccgc gactacgtta tcgaaaactt cgcgccggaa cccaaatcgg gtcgccgcag
     5521 gcgtgtcatg atgctcctct tccagggcga agacgccgtc cgcagggtcc gaaccgttgt
     5581 gggcaacttc agcccgcacc gccgcggcgg gcaaacgatc cgcgacactt acggcgatct
     5641 ggtcctggat gccaacgacg aggtgcgtta tttcgaaccc gcggttctgg cggctccttc
     5701 gctcgacgag gcgatcgcca agctcaagct ctgggctcgc tactcggaca ccgaaggggg
     5761 cgtcctcgat caggtgatca gctacgcagc ggacgagcag tcggagcgga cgcttgtcct
     5821 gctgaaaccg gacaacttca agttcgccac ggggcgtccg gggaacatga tcgacttctt
     5881 ctcgcgcaca gggttgttca tcgtcgggat caaggtgcac cggatgagca cggcccaggc
     5941 gatggagttc tacggcccgg tccgggagat tctgcggacc aagctcaaga gtgttgtggc
     6001 gacgcgggcg aaggaagtgc tcgagaagga actcggattc gccattggcg cgtcggaatc
     6061 gcagcaactc ggggagttgc tcggcccgct cctgggcgag aaccagttcg aaaacatcgt
     6121 ccgcttcatg gccggacgca gccccagcga gtgtgagccg gcccaaatga cccagccagg
     6181 caccgagaag tgcatcgccc tcgtctatga gggcgtcaat gccgtcgcca agatccgcga
     6241 tgtgctcggg ccaaccgatc ccaccaaggc gccgccggga tccatccgac gggagttcgg
     6301 atccaacatc atgatcaacg cggcccacgc cagcgactcc gccgagaacg ccaagcgcga
     6361 gatgcgaatc gtgaatgtgg cggagaataa cttccgacag attattgaag gtttttacgg
     6421 gcgggtgtca tgacgaccag tcacgaccaa tgggacccgc gccgcacccc agaagccggc
     6481 ttcagcccga cggcggttga tccgggtcgg cctccgacgc cgcgatgaac gccgccgcga
     6541 ctcccccaaa ccacgacggt cccccttggt ggcgtctccc aaaccggtat caatggtatg
     6601 tcttcgccct ggccgctttc ggctggctct tcgactgctt tgaccagcaa atcttcacac
     6661 tctcgcgctc gatcacgatg cgcgaccttt tgccccacgc cgacaccctg acccagacca
     6721 cccgcggcgg ctacgccacc tcgatcttca tcctcggttg ggccaccggc gggctgttct
     6781 tcggaatgct cggagaccgc tggggccggg ccaagacgat ggccacgaca atcctggtct
     6841 acgccgcgtg caccggactc agtggcctgt ctcaaagctg ggggtacttc gccctctttc
     6901 gcttcctggc cggggcaggg gtcggcggcg agttcgccgt aggcgcggcc ctggtcgcgg
     6961 aagtcatgcc cgacagggcg cgcccgcaca cgttgggatc gctccaggct ctctcggcca
     7021 taggcaacgt cctggcagcc gtcagcctgg gcgtggttgt gccggacgaa agactgggct
     7081 ggggctggcg tgggctctat ttcctggggg cgttgccggg cttggtggcg gtggtcgttt
     7141 tcctgcgact caaggagccc gaaaagtggg tggccgcgcg cgcggcggcg gcccggggcg
     7201 ggtcgtcccg ccagtttggc agcatcaagg aactcttccg catcccggtc tggcggcgca
     7261 acacactcgt cggtgtcagt ctggcggtgg cgggtgtgct cgggctctgg ggcgtgggat
     7321 tctacagccc cgaactgatc gactcggcca tcacgacggt cgagacggag acccggccca
     7381 agatcgaggc tgttctgcgg gccgataccc cggcagccca ttccgccgcg ttggcagggc
     7441 tcagtgcgca ggaaaaccgc aagtacctcg aactgctttc gcgcatcggc gccgccgaca
     7501 cccggtcgac ctcgactgat ccagcggcaa gcccgctcac aggcgatcag aaggagcggc
     7561 tcgcccaact cctggcgggt gcgattaccg aggaccgaaa aacacacctc aagtccgtcg
     7621 gcggcatcct ccaacaggtc ggctcgttcc tgggcatcac gtttttcacc gccatcgcgg
     7681 cgcgcctggg aagacgactg tcgtttctga tcgggttgac aattgcgtgg gcgagcctga
     7741 tcctcgtgtt cggcaccttt caccaggccc accaaatcta ctatctgtgg ccaattctcg
     7801 ggttttgcac cctggcgcct ttcggcggct acgccatcta tttcccggaa ctcttcccga
     7861 cccggcttcg gaccaccggc atgagttttt gctacaacgt ggggcgctac atcgccgccc
     7921 tcgggccatt cgtcctggga ccgctcgcca gggcgctgga cggcctgacg gcgatccccg
     7981 gatttcgcct ggccgcgatg acgctggcct gttcctatct gttgggaatc gtcgcgctga
     8041 tctgggcgcc agagacactc gggcggcctt tgcccgacga cgagcgggag aacaccgggg
     8101 cctgagagac gacaatagag cccgaagatg tcgcaggcgt cgtaaaccgt tcgaagttca
     8161 ctcgcgcgag agacggcctg cctgccgcaa ctgctgccgt ttactttcgc tggattttgc
     8221 cgggattgcg cgccacctta agcccatgaa gctcatcctg acgacgcata acgtcacgct
     8281 gaccagcgcc atcgaagaac acattctcga gaaactcgac aagctcgagc atttcaaccg
     8341 ctgggcgatc gatgcccggg tcaccctcga ccacgaccat accaaggccc ccaaaaaaca
     8401 gtttggctgc tccgtccgtc tgggtgtccg tgggccggat ttgttcgccg aggattccca
     8461 gaacgatctc tacaccgcga ttgacatggt ctttaagaag atcgaacagc agattcgcaa
     8521 acggcgcggc aaacgcgaga ccgtcaaaca tacccatgct gcacgcctga agcgcaagcg
     8581 ccaggacacc gaactctaga ttccttgacc gttgaaactt gttggaaaca catcgagttt
     8641 ctgagctgcg ttcaagggat cgaggattct ctgcgacgag gcgaggggga gaggagcggg
     8701 atggccgtcg agtccttgaa ggtcttgcgg gcgcgcgttg tcctgccgat ccgccagccg
     8761 cgcctcccga atgccgcggt cgtcgtctcc ggagggcgta ttcaagccgt gggccagtgg
     8821 aaggacctcc gaccccactg cgcgggatcc ccgacggatt tgggcgaagt ggccctgctg
     8881 cccggtctgg tgaacgcgca ctgccatctc gactataccg acatggcggg ccaattggcg
     8941 ccttcgcgct cgttcaccga ctggatcaaa cgcatcacgg aactcaaagg cgcatggaat
     9001 cgtgacgact ttgcgcgttc ctggtggcac ggcgcccaaa tgctgatccg aaccggcacc
     9061 accgtcgttg gcgatgtcga gtccgcgcct gacctcctcc cggagatgtg gcaggcgact
     9121 cccttgcgcc tcacgtcgtt cattgaattg acgggcgtca agagccgccg cacccccagg
     9181 gcgatcctcg acgaagccct cgccgcaatc gccgcactcc ctcgcgggcg ccaccgcgcc
     9241 tttctctcgc cgcacgcccc gtattcgaca ccacccgaac tcctgcggcg atgcggagcg
     9301 atcacgcgcc ggcgccgatg gcggatcacc acgcacgtcg ccgaatctga agaagagtac
     9361 gcgatgttca ccaggagagg cgggccgatg ttcgattggc tcgagcgcaa ccagcgcgat
     9421 atgtccgatt gcggcggaat ctcgcccatt caacacctgg cgcgccagaa tctcctcggt
     9481 gaccgtttgc tggccgtgca tgccaattac ctcgaccgcg gcgatgccga cctcctcgcc
     9541 ataaaccgcg tccatgtgat ccactgcccg cgcagccacg cctacttcgg ccatcgtcct
     9601 ttccccgtca cggcactgat gcggcgcggt gtgaacgtgt gcctgggcac cgacagcctg
     9661 gccacggtcg caacgcctcg gggacaaacg gccgcgctcg atctgttcga ggaaatgcgg
     9721 gtgtttgccc aggcgcaccc caggtggcca cccgacgccg tcctgcgcct ggccacgctc
     9781 aacggggcgc gggccctggg cttcgcaggc cgcgtcggcg aaatcactcc cggcgcgtgg
     9841 gccgacctca tcgccattcc gtcgagtgtc ggactgcgcg aggtcgccgc ggccctggtg
     9901 cacaaccggc agccggttgt ggcggcgatg atcggcggcg agtggatcgc gggcttcccg
     9961 ggcgcatatt gcaccccgtc agtgcaaagt gcggtcggac tccgcgaagc tctgtgaaac
    10021 acgcgtctac cggagcagga tccgcatccc tgccaccagg gtcagcaccg tcaccggcag
    10081 accgaatttc gcatagtccc agaatccgac ctcgacgtgt ttgcgggcgg attcgacgac
    10141 gatgatgttg gcgaccgatc cgaggatggt gaggtcgccg gaaaacgtcg ttgccagggc
    10201 tatcacctgc cacatccgct tcggctcgac gaagttcgaa atccatttcc cggccaccaa
    10261 aacgaaagga acattcgaga aaatgttcga gccgagggcg gagaaccagg cgagattcca
    10321 gccctgggcg gcgagcgtgt gaccgaagag accgcgcaca tggccgtaaa tctggtcggg
    10381 caggccggtg gcgttcaaag cctcgaccac cacgaacagg gcggcaaaga aaaccagcaa
    10441 attcaagctc cgcgctcacg ggttcaacag ccggcccggc ccatcccacg atgccgcgcc
    10501 cgccggggac catccgcggc gtgtgccagc cggcggcaac ctctgccaac ccccgatgcc
    10561 agcgtcagtc caaatgcgca tccagaccaa tgcagtcggc gctcgacacg cgccgtgcgc
    10621 cgccctatgc agttgtcctt tcagacaaac cggtctgttc ccccgcgacc atgtcaaagg
    10681 ccgaatccaa gatggactcg cgcgagcgcg tccgatgcac catgcggcat cttttgccgg
    10741 atcgcgtgcc ggtgttctgc cagctcgcct tgggccacta cttcctccac agcggcatcg
    10801 ccccgcacga aatttggttc accaccgagg gcttcgccca ggcgttggtc cgcctccagc
    10861 agcgctaccg gttcgatggc atcctcgtta acctgcccgg acgccccccg gacctgctct
    10921 cgcgcgtcca gcagatcgac gccacggatg aagggacgcg acttcggtgg gccgacggca
    10981 gttgcacaac cgtcccaccc gacgacaacc cacagcatcg cctcgccacc caggcttcat
    11041 tgccgcgcgc ggacttcgcc cggctcgatc cggatcggct tgacgctctt gatgccctca
    11101 caggttattg gtggaacgtc tgtcacgtcc cgtggatcga cggcaaaacc tccccaggcc
    11161 tcctgcgccg gatccccggg tacttcggag acaccatcga tcgggtgcgg gcgttgacgc
    11221 gcggtgcggt ctcgatccat ggcgaagtgt tctcgccatt cacgcatttc atggagctgt
    11281 tcggatacga ggaagccctg atggcgctgg tgaccgatcc gggcaaggcg cgcgcctttc
    11341 tcgaccggtt gacggagtcc tcgatcgcct ggggcgtggc tcaggcgcgg cgcggggcgg
    11401 atgccgtgct cgtgtcgtcc gcgttcgcgg gcgcaggttt cctatcgaaa gcggcctacg
    11461 ccggttttgt ccttccgtac gaacgcaggg tcgcacgggc aatccgcgcg gcgggcgtgc
    11521 ccgtttacac ccatacctgc ggacggatcg gagatcgtct cgatctcatg atcgagaccg
    11581 agacgcaggg tttggacacg ctggatcctc cgcccttggg tgacgtggat ttggcggcgg
    11641 caaaacggca gctcgaagga cgcctcttca tcaaaggtaa tatgaactcc gtcgccttgc
    11701 tggcgtatcg cgcccccggc gaagtcgagg ccgaggtgcg gcgttgcctc cgcgatggcg
    11761 cgccgggggg cggctacatc ctcagcaccg cctgttcggt ggccccgcgg gtcgagccct
    11821 ggaaactcga gttgattgcg gcgctggccg agaaggagcg ctactagccc ggatgtttcg
    11881 caaagcatcg cggagtctgg catccaggct gtggcaaaac cacgcccaga aaaggaaccg
    11941 agagcattct gtcggtcctg tgggtggtct gccgtgagac aatgtgttgc agttctctat
    12001 ctctcgaacc gggcgcggtc ttgcttctct agcccgcatg gctttcatct tgcgaagtct
    12061 catccacacg cgcacagacg gtgtgtaaga aacgggcgag ccggcggtga ctgtcctcat
    12121 ttcccggcca ggccgcgcag ataggcgaaa atgagatcgg gcaaatctcg actgtaaccg
    12181 ccttcaagca ggctgaaggc cggcacgccc aattcccgaa tcgagcaacc cagccagcca
    12241 aaatcctcct cttgcagggc cgcgtcgctg atcggatcgc cgcagtaggc atcgaatccc
    12301 gccgatacca ccaccagttc gggcttgaac cgctctaaat cttcgagcgc cttggccagc
    12361 gcctggcggt agtcctctcg agccgcgccg ggcgccacgg ggtagttatg cgcgttgcca
    12421 taagaatcgc tcccggtgcc cggataacac gggaattggt gcacggaaaa gtaggcgcat
    12481 tggtcctgac ccagcaggat gtcctcagtg ccgttcccat gatgcacatc gaagtcgtaa
    12541 acggccacac gcccgacacc tttggcccgc gcctccatca ccgcaatcgc cgccgagttc
    12601 agatagcaga aacccatcgc tcggtccggc gtcgcatgat gccccggcgg acgcagcagg
    12661 ctgaaggccg tcttgctctc gagagccctc tgcagggcgt agagtgcccc gccagcgctc
    12721 cgctccgcgt gcgcgcgaat gtccggatac gccgccgtgt ccgagtcgaa gtccaccgac
    12781 accttcagcc gttcgagaag ggaccgcttg tgggcgcgct cgagctgcgc ctcggtcacc
    12841 ggcgccggct ccgcccagac caactcgatt tcgctctgcg ccctaagacg ttcgacggtc
    12901 aggctgatgc gctgcggccg ttcgggatgg ccgaattgct gataggcggt gcaccgactg
    12961 tccgtgacga taatcatgcc atgcttgtct ccccggaacg agccaggagc ggcttcgcgc
    13021 aaagactctg taacattcgc tgccgagctc gccaagcgcg aagaacggtt ccccgggagt
    13081 cccagtgcat ctgtacgcgc tccattcagt tggccgccgc atggccaagg cgtccgggag
    13141 ccaattcccg tagcagcccg cgcaggttct cccgcgccca gcgctggaac gtcaggttac
    13201 agagaccgca cacgatcagc cagagcagag gcagggcgag cggcccctga acgcccgtga
    13261 agaacagcgc gaacaggaag accgcccagg gcacacaaag gatgcgcacc gccgtgccga
    13321 cgaatgcctg gttggctttg cggctcgaca gccccagcca cagaccaacc cacgccagcg
    13381 catacaggtc ccagatgaag acaagcgccc cgaccagtgt catcagcccc atgtccgatc
    13441 ccagtccgtt cagcccctca ctggctgcca gcgtgaagac ctccacgagc aggatggcca
    13501 gcgcaggcgc gccgaacaac cgcctcaggg acagccagtg accccgcagg atctcggcca
    13561 cggacaatgg tgtggtcagg agcagttcga gggccccggt ctgccgttcc tgcgtccagc
    13621 gctggctcgc ctcggaggcc atcagccact tcagcggggc gtgcagaaac agggcgatca
    13681 gaaacgtgct gttccaggtg tcgctcgcca tccccatccg ccagcgccac accagccaca
    13741 cgaacaacgc caaaccgaca gctccccaca gcagccggac cttgagctgg ttgcggctcg
    13801 ccagccacag aaccggattc cgatccagca gccgccgacg cgagtgttgg cgctcgatcg
    13861 ccgtgccgta attgagcatg tgccagcggt gccgccacgg cacgcccgcc ttcagcacgc
    13921 cctgctcccg ccacgcccgc tgcgtcagcc aggccgcgct cccgagaaac agccacgcca
    13981 gaccgtgcgt ccccgacaac gcccagccca gaacgacgcc ctcccggttc gccgccacgc
    14041 tcgacgccag ttcgagtgcc gagctggggt tggcggccag cagaatgcca aggcccgtgt
    14101 agctcccgat gaccaaccgc agccacgggc cgacaccggc aaacaggaac aggatgaaca
    14161 cggcgagcgc gaacgcgctg cgcgcctggg tgctcgccgc cgaaacgcac atgcccaccg
    14221 caagcgagaa gaacagcgca ttgccgagaa ccagggccac cgccccgaat tcgatcaaac
    14281 gcacgccgcc aagcaggatc ggaatcgcca ataccggcag cgccgccatc aggctgaaca
    14341 gcgccgtcac cgacgcagca accagcttgc cgcccacaat gtcgtaactg cgcaggtcgg
    14401 tcagaaacag cagccccaag ctcccgtccc gcctttcctc gctcagaaca tccgcggtaa
    14461 acagcggccc cgcggccaga caaaacaggt atcccacaaa agccagcgta tcgaaaagct
    14521 ccctcccgat ccgcgtcccc cccgtccatt cgccatggta ccacaggttg atcgcgccca
    14581 ccgccaccgc caccgccgcc gccaccagcc gcgcccagaa ggcgcccggg cggcgcgacg
    14641 ccacgagcaa ctcgcgctgg acgatcggga aaagcgtcat ggcgcagtcg aggcctcgct
    14701 cgaatgattc ccagcacccg tcttcatgtc tgagttcgac attcccgaaa gatgcacctc
    14761 acgacaagat tctggattcg ccggccaccg cgtgcggcac ggtccatgaa acatgcggac
    14821 gcgaccgccg tcgtctgaat cgggcgccaa actcccattc gtgggtctgc gccgccgcgc
    14881 tccggagatg ccgccgcagc cgcccgcgcg cccagaggat gatcagcaca ttcttcccca
    14941 cccagcccaa ggttcgaaac accatcgagt aaccgaccat tccgccccca agcgtccgca
    15001 ggatcgtgct ggtgttgagg taggtcatga ccttcaccgg cagcagcgaa gagagattcg
    15061 ggaacaccac gcccagcgtc catggcccca acacaaccca ggcaaaggtc ttgagaatcg
    15121 cgaaggtcgg gcggcgcaac gccaggctca gccacgcccc cagccagcag atggcgaaca
    15181 catcggtcac gaaattgagc aggctgctcc cggcgctgaa ggcgtactgc tggagcaggc
    15241 cgtgactgaa cgggccgcgc tgggccgcaa gccgccagtt ctccccgatg tagaaaatgt
    15301 gcagcaaggc gatcatcgcg accggcccgg caaacaaaag ccaaagcgcg cgccaatgac
    15361 cgcgcaacag ggtgcgcgcc ggcatcggcg aggtgagaat caactcgaga gcccccgtgc
    15421 gccgcgcctc gagccagaaa cggcaggcca tcacgcacaa ccaaacccgg ttcgtgaaca
    15481 ccaacccatg ggaaaaatag gtgtggaagg ccgaggcccc ggtgttggtg cggtagccgt
    15541 gcgtcaacca gtagacgttc accgcgacca gcgacaccaa cgcccagaaa acccaatgcg
    15601 gccagcgcag ccgggcggcc accgcataca ccggattcgt ctcgagccgc cgcttgtacg
    15661 tcgcccgcca ccccgccgtg tagcccagcc gccacagcca gcgatgcgca atcggtttct
    15721 cctccggact ctccctccac agccgcggca gccagatggc ggtcagcccc atgcacaggc
    15781 agctcatccc gcacacaaac cccagcgaaa cccaaaacgc acccggcgcc gtcgcatacg
    15841 cgcgttcgct cgccagatac cccgtgtagg ccggactcat cagggccgcc ggcaacgggc
    15901 tgggcagcgg ccgggtaaac aaacccgagc tgaacaccgc cgccagaccc ggaatcaacc
    15961 cgctcacgcc cagaatcgcc accgccgatc cgagaatggc tttggattga tcgcggctca
    16021 gcgtcgatac aaacatcccg gccgtcagcg agaacaggat ggcgttcacc accgccaggg
    16081 ccgtccgcac gtactccccc gctgtcactc cccccatcag cagcggaatc cccagcgccg
    16141 gcaggatggc cagcaacccg taaaagcccg ccagcgagtt cgccagccac ttccccaaca
    16201 ccacgtcgta actccgcagt tcggtcagaa acagcagccc cagcgtcccc tcgcgcttct
    16261 cctcgctgat gctgtccgcc gtgatgaacg ggccgaccaa cagacagtag ccgaacgcca
    16321 gaatcgtcag gcacgagaac agccaccgcc ccgccgtcac gggcgtctgg gtcttggcaa
    16381 cccacagagc aatccaggcg ctgaccacaa atgcggccgt cgcggcggcc agtcgcaccc
    16441 agtgcgtctt cgatcgccgt gccgccgtcc gcaattcccg gtccaccacg ggaaacagtg
    16501 tccacacgtg cccgagtcta ggagcgcagc cccaaactcc aaggccgaat cgcgcaagaa
    16561 cacaggcata tgggagatta tggttgcctc gcgccagaac gttgctagat tatccgctcg
    16621 aatcgaagcg gagcagtccg tgtattactt cgtttgatgt acgcagcagt gatgacagcc
    16681 atcgacgaat cgctcaggaa caaatcgacg ctctaccagg atttcctggc ggaacgtgac
    16741 gaaatcctca gacacaagtg gatcgagtcc gaaaaggcgg ggtacgacat cggcttcgag
    16801 cgcgcgttga cggactggag cgttcatcac cgcggcccat ggcggcgctg gcatcagagc
    16861 cggcgagccg gcctgtcatg atccggactc ctgacaggtg ccgcccgcgc tgaaggttcg
    16921 cggtgcggcg ccgattcgcc tgttcttacc tggtgtctcg gagattccca caagcattct
    16981 tcatttgctc cttgccgcgt cctttcatca ctcttcgttt ccactatgcc tacccgctcc
    17041 cataaaaacc gtgtcgtcat ggattctcga gccatcgacc tggctgtgcg ccgggttgcc
    17101 cgcgccatcg catcgcgcgc cgaaggccac gcccgagtcg tcttggtcgg catccagacg
    17161 ggaggtgtct ggctggcgcg gcacctggcg caacacctcg agcggctgtg gtccgccccg
    17221 gtgccggtgg gtcaactcga catcagcatg caccgggacg acctcgatca gcggttggct
    17281 ccgcaggtgc acccaactgc gatcggattc gacgtcacgg gcaagacggt cattctcgtc
    17341 gacgacgtgc tcttcagcgg tcgcaccata cgcgcggcca tggatgccct caccgatctc
    17401 gggcgccccc gccgtatcca gttggcggtc ctgattgacc gcggacaccg cgaactgccc
    17461 atcgaagccg acttcgtcgg caaacggatc gccacaggcc cgaccgatcg ggtcgacgtc
    17521 cgcctggccg acaccctcgc ggcgtcacaa gtcgtccttg aaaaagccgt ccgaagatag
    17581 ctcaatgccg gcatgctctg gacccgcaaa gacctgctcg atctccagtc cctctcggtc
    17641 gaggaaatca ccgccgtcct cgacacggcc cgcgccttca gagccgtcgg cgaacgcacc
    17701 atcaaaaagg tcccggccct gcgcggcaag accgtgatca atctcttcat cgaaccctcg
    17761 actcgcaccc gcatcagctt cgaactcgcg gcccagcgcc tcagcgcgga cgtcatcaat
    17821 tttaccgccg aggcctcctc gctcaagaag ggggaaaccc tcaaggacac cgcccgtaac
    17881 ctcgaagccc tcaatgcgga tttcatcatc atccgtcata gcgcggccgg cgccccccac
    17941 tttctcgccc gcgtcctgaa cgcgcatgtc gtgaatgccg gagacggcgc ccacgagcac
    18001 cccacccagg gcctgctcga tatcttcacc atgcgcgaga aaaaagggag cctccagggc
    18061 ctgcgcgtca ccatcctcgg cgacatcctc tacagccgag tcgcccgctc gaacatctgg
    18121 gctctgagaa aaatgggcgc ccacatcacc ttgtgtggtc cccccaccct cgtcccccgc
    18181 atcttcgaac agatggagtg ccgggtcacg tccgatctcg aagaggccat cgccgatgcg
    18241 gacgtcatta acctgctccg catccagcac gagcgacagc gcaagaccat gttccccagc
    18301 ctcgccgagt acagcagcct cttcggcatc aaccgggccc gactcgccgc caccaagccc
    18361 gacgtgctca tcatgcatcc aggccccatc aaccgcggcg tcgaggtcga cagtgaggtg
    18421 gctgacggcg aacgctcggt gatcctcgaa caggtgacca acggtctggc catccgcatg
    18481 gccgccctct acctcgtcaa cggaggacag gcggcgtcga ccgagactcc ggaacaggcg
    18541 gccgcctagc agcctgtcgg acttagatcg agaagcaacc cttcaggaat ttaaatgaaa
    18601 tcatcgtgcc gcccgacgcg gtcacgtcgt gccgtagagt tcggcgcctt tgtcgacaaa
    18661 ctcgcgcgct ttgcggtcca tatcagactg gatcgcctgc cgttcggtga catcctgttc
    18721 cgccgcggac ctccggacat cctcgctgat cttcatcgca cagaaacgcg ggccgcacat
    18781 cgagcagaag tggacggtct tggcgtcttc ctggggcagc gtctcgtcgt ggaaggcgcg
    18841 ggcggtttcg ggatcgagtg acagattgaa ttggtcttcc cagcggaact cgaagcgggc
    18901 ttggctcagc gcgttgtcgc ggtactgggc gcctggatgg cccttggcaa ggtcggcggc
    18961 atgggcggcg atcttgtagg cgatgacgcc gtctttgacg tccttcctgt tgggcagccc
    19021 gaggtgttcc ttgggtgtga cgtaacagag catggccgtg ccgtaccagc cgatcatcgc
    19081 ggcgccaatg gcgctgctga ggtgatcgta gccgggggcg atgtcggtcg tcagcgggcc
    19141 aagggtgtag aatggggcct cgtgacacca ctcgagttgc ctgtgcatgt tctcctcgat
    19201 cagatgcatc ggcacgtggc ccggcccttc gttcatcacc tggcaaccgc ggtcccaggc
    19261 gcgccgcgtg aggtcgccct gcgtctcgag ttcggcaaac tgcgcctcgt cgttggcgtc
    19321 cgcaatcgcc ccgggccgca ggccgtcccc gatgcttaag gccgcatcat acgccgccag
    19381 aatctcgcag aggtcgtccc aatgggtgta caggaaattc tcccggtgat gcgcgaggca
    19441 ccatttggcc atgatactgc ccccgcggct gacgatcccc gccgcgcgat ggacggtcag
    19501 cgggatgaag cgcagcagga cgcccgcgtg aacggtgaag taatccaccc cctgctcgca
    19561 ttgctcgatc agcgtgtcgc ggtagatctc ccacgtgaga ttctccgccc tgccgcccgc
    19621 cttttcgagc gcttggtaga tcgggacggt cccgaccggt acgggcgcgt tgcgcagaat
    19681 ccactcgcgc gtggcgtgga tgttcggccc cgtggacaga tccatcaccg tgtcggcgcc
    19741 ccagcggatc gcccagcgca tcttctcgac ctcctcctcg atgctcgacg ccacggcgct
    19801 gttgcccagg ttggcgttga tcttcaccag aaaactgcgt ccgatcacca tcggctcgct
    19861 ctcgggatgg ttcacgttgg ccgggatgat ggcccggccc gccgccactt cgtcacggac
    19921 aaactcgggc gtgatctttc gcggcacgcg cggcctgaaa cggctgaaca ccgacggggc
    19981 cgcggttgga tcgaccgagg tcggcccggc atgctggtga cgcaggtcgt tccgggcggc
    20041 gtcccgtgcc cgctccgcct cgagccggtc aaatcccagg ttctcccgga tcgcaacaaa
    20101 ctccatctcc ggcgtgatca ctccgcgccg cgcgtattcg agttgggtgg ggcgctggtt
    20161 gggtttggcc cgaagcggac gcctgggagc aaagtgcgaa ggaccgccgg gcggcgctga
    20221 aacctctggt gcgccggcac gatcgagatt tgcagacgac cagccctggc cgccgctctc
    20281 gtattcctcg acatcgccgc gcgccaggat ccattcgcgg cgcagcggcg gaagcccctg
    20341 gctgacatcg ccgtgaaagt ccggatcgcc ccagggcccg ctgcagtcgt aaacccggac
    20401 cggtgcgttg ggacgaatcc gcccgttggc gagccgggtg ggcgagagcg cgatctcgcg
    20461 catcggcacc cgcaccgacg cgtgtattcg gccgggcaga tacacccgcc gcgagccggg
    20521 aagtgaatcc gcgcgtattg acttcgacct gtcttcagtg gcgatcatag tccgtgagca
    20581 tcgggtcagc accgccggac tcgactgcgt gaccggaggt gcgttcaggt tggagacacg
    20641 gggaaaggca ggcctcgaat cccttcgccg gcattacccg gatcaggttc aacgggtcgg
    20701 caacgcccgt gcgcgtcgcc ctctcagccg tcaccagtgg cctggcttcc tggctccccg
    20761 aacacggcga acagtaagag tgcagtgcag cgaggtcaag cggacgcgac cacggtcgtc
    20821 attgacacca aagtccagac atgcaaggtc ggttgcggac tgcagcacga gagaatcatt
    20881 taatcgagac cgcctatgcc ttcaccccac atgaacacac cggcgcggcc tgatcccttg
    20941 acctcgatcg cgggcactgc cccgtcctcg ccgaacccga cggtcagccc tttcctttgc
    21001 ctcgccctgc tcgccctctc cctcgaggcg tctctgcccg ccctcggggc cccggaaggc
    21061 gccaccgtcc gcgagtatcg gaagacgttc cggacctacc ccttttccga cccggatccg
    21121 attccgaatg gcggccagat ttacccctac ttccgttacg acggcttcac cgaccgcgcc
    21181 gaggaccgcg agtggacggt ggtcgaactc gagaacccct ggattcgcgt catggtgctc
    21241 cccgaaatcg gcggcaaggt ctggtcggcc gtcgagaagt ccaccggcaa ggaattcctc
    21301 tacggcaacc aggtggtgaa gttccgggac atcgccatgc gcggcccctg gacgagcggg
    21361 ggaatcgagg cgaactacgg catcatcggc catacgccga actgcgccac gccggtcgat
    21421 taccggacac ggcggaaggc cgatgggagc gtctcggtcg tcatcggcgt cctcgatctc
    21481 ctcacgcgca cgccctggcg gatcgagatc gcgctgcctg cggacaaggc ctatttcacc
    21541 accgcgtcgc tctggcacaa cgcgacgccg ttcgagcagc cgtactacac ctggatgaac
    21601 gccggcatca aggccgcggg caacctccag ttcgtcttcc cgggcacgca tcacatcgat
    21661 catgacggcc gggctcaacc gtggccaatc gatccgaaaa cggggcggga cctctctttc
    21721 tacgagcgaa acgacttcgg ctcctacaag gcctaccacg tgctcggacg cggcgctgac
    21781 ttcttcgccg ccttctggca cgacgaggac ttcggcatgg ggcgcttttc ttaccgcgat
    21841 gaaaaactgg gtcagaaggt ctggatttgg ggactctcgc gcgaggggat gatctgggag
    21901 aagttgctca ccgacaccga ggggcagtac gtcgaagtgc aatcgggacg ttccttcaac
    21961 caggccggcg gaggcagttc gaggacgcct ttcaaacatc gcggctttct gccttacgcg
    22021 accgacacct ggaccgagta ctggttcccc gtcaagggca cgaaggggct cgtggcggcc
    22081 tctcccttcg ggggcctgaa cgtgcggcct cgcgaagacg ggctcgaggt cctgttctcc
    22141 cctctgcagg ccgtcgacga cacgctcgag gtgctcgatg gcgagcgcat cgttttctcg
    22201 tcgctcgtct ccgcgaaacc cctcgagccc tggaccgcaa tcgttcccgt gacggcgccc
    22261 gtcgagcgcc tgcgcgtccg gatcggcgga aaccgcctcg agtggaaggg cgacccggga
    22321 gcgggagaac tgtcgcgtcc gctccagtcg ccccccgatt tcgactggac ctcggcctat
    22381 ggcctctggc tcaaaggcaa ggaactgctc cgacagcgct cctacgcccc cgctcgtgag
    22441 gcgctcgagg cctctctgcg caaggagccg cacttcgtgc ccgccctcgc cgacctctcc
    22501 cttctccgcc tctttgccat ggatgccaag ggcgccttcg acctggcgcg gaaagccctc
    22561 gccgtcgaca cctacgaccc ggccgcgaat tactgctacg gcctcgccgc ccggaggcta
//
LOCUS       DAOQRO010000005        22233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1554_length_22233_cov_5.290964, whole genome shotgun sequence.
ACCESSION   DAOQRO010000005 DAOQRO010000000
VERSION     DAOQRO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 22233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22233
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1554_length_22233_cov_5.290964"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..954
                     /gene="pgsA"
                     /locus_tag="PKK20_00425"
     CDS             358..954
                     /gene="pgsA"
                     /locus_tag="PKK20_00425"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNU98387.1"
                     /translation="MNLPNKLTVSRFALTVVFVLAVLSRGPVNDTLALVFFCAAGITD
                     YLDGRIARRRGLITNFGILMDPLADKVLICTAFIMLLERGRMNPSAPVTVEGWMVVLI
                     VARELTITGLRLLAASKRIVLAAEGYGKHKTVSQIVAIISLLVLDAHVEWWSWLRGLF
                     EWWVPAFTQLALYMTVVLTVTSGALYLWRNRALYLDDM"
     gene            944..1471
                     /locus_tag="PKK20_00430"
     CDS             944..1471
                     /locus_tag="PKK20_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNU98388.1"
                     /translation="MTCERKWRDALVLWLARGFGVGRVPVAPGTFGSVVGLAWTLVLL
                     ATRSVWWYGLGVAAGLALSVVVCGLAERLLRERDPGSVVLDEITALPLCFAGWIGHHL
                     WVTGAMPAPEVLVTGRGWLVLAAGFVAFRVFDVLKPWPIRQSQAMPGGWGVTIDDVLA
                     GLASNIIWMPCAWLS"
     gene            complement(1537..2943)
                     /locus_tag="PKK20_00435"
     CDS             complement(1537..2943)
                     /locus_tag="PKK20_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98389.1"
                     /translation="MPHKITSAHGASLSRRDFLAVAGAAGAGGALIAKTAAAAAADPP
                     PPTPQPAQQRVFGLRHPALDMVRIGFIGVGSRGSSLLGNLLELDGLQIKAVCDIVPER
                     VEAARRRVAAKNQPEPEGYSEGEMDFERLCQRDDLDLVYIATPWDWHVPMAVSAMRHG
                     KHAAIEVPAAMTLDDCWRLVDTAEQTRRHCLMLENCCYGEIELMVLRMVRQGVLGELT
                     HGEAGYLHEARDWLLREDSPANWRRRFVATLDGNLYPTHGLGPVAEYMGIHAGDTFDY
                     LVSMSSLELALSRRLEARSGASTSRGRAFACGDINTTLVKTALGRTIMIQYSLAQSRP
                     YSRINLIAGTTGTFCDYPPRLHLDGQGEDWTTELQPYHDKHGHPFWKSLNAQARQSGG
                     HGGMDYVMNWRLIQCLRRGLPLDMTVYDAAAWSSIVPLSIASVARRSAPVTVPDFTRG
                     TWRQQAPSALGDDWPAPV"
     gene            complement(3057..4550)
                     /locus_tag="PKK20_00440"
     CDS             complement(3057..4550)
                     /locus_tag="PKK20_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNU98390.1"
                     /translation="MKILLVSPQTPDTFWSFRHVVRMVSKRAAFPPLGLLTVAAMLPS
                     DWSLRVVDLNVERLRDEHLRWADYVMLSAMRIQRASVLDVVARCAAAAKPVIGGGPLF
                     TTGHEEFSEVPHVVLGEAEDVMPELVRDLEQGTLKPVYRASGYPDVTRTPIPRWDLID
                     LRNYVTMAVQFSRGCPFDCEFCDIIVMNGRVPRTKTPAQLVAELETLRRAGWKDMVFV
                     VDDNFIGDKRRARALLRTLIEWRTGTGAPGGFLTEASVNLADDPELLRLMVQAGFTKV
                     FVGIETPSAESLAECHKMQNRGRDLMSAVQTMQQAGLEVMGGFIVGFDSDPADIFKRQ
                     FEFIQRSGVVTAMVGLLTALPRTRLYERLKREGRLERESCGNNTQAELNFRPKLNRDF
                     LLSGYRDLMNRLYEPRAYYQRIRNFLRQHQPRRGPALRLSPDDVRAFLKSFWVLGVAH
                     RGRRAYWRLLWTTLLLRPHQFRQAIEMAIIGHHFRRVATSLDRTGDS"
     gene            complement(4621..6408)
                     /locus_tag="PKK20_00445"
     CDS             complement(4621..6408)
                     /locus_tag="PKK20_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNU98391.1"
                     /translation="MNRRLPIPCASTPRARWSPRPWLLAAVLLVPIAGLADDATRKTA
                     AAWRAEHRLIDLHQHVDSSETALRRDMRLMDTVGVGLVVNLSGGFVTHKPGEKSAFER
                     NKELADRLFPGRFLYYMSLDFAGWDEPDFSERAVQQLEEGYRLGAAGLKEYKRLGLYL
                     KDKAGRLIRVDDPKLDPVWRRCGELGMPVSIHVADPQAFWKPYNAENERWRELKDHPQ
                     WWFGDPAKYPPFHELLTALDRVIERHPQTTFVCVHFANNAEDIDWVEQALDKRPNMHA
                     DLAARIPEIGRHDPARVHRLFVKHQDRILFGTDFMVYANLTLGSGGSGPPPTDEDAIE
                     FYEKHWRWLETSDRQFEHMTPIQGDWKIDAIDLPASVLRKIYFDNARRLLVRSLPLPV
                     VQAKKIDRDFKPNGRLDEPAWRAASPARLEYLLKDGSARPDLATTARILWSDSHLYFG
                     FEAPYETLATFAPVLKDKERLGLWERDVVEAFIGTDPTRPDHYTEFEVAPTGERLDLA
                     IRGQDKDFDWSSGFESAVRLNRRTKVWTTEFRIPLAALSPSKPEPGTRWRLNLYRHAA
                     TERAFLAWSPTATATAHTPAKFGFLELVH"
     gene            complement(6504..7541)
                     /locus_tag="PKK20_00450"
     CDS             complement(6504..7541)
                     /locus_tag="PKK20_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98392.1"
                     /translation="MKQAFTWFAMKRPFTSFLGAWLGLAMLLAPAAHAEDVRAIFLFK
                     TVWYEQNDAGDPVPIPSADEPYDITADVYLTDETLSDPEWYWWISGMTVRTPSSQTHG
                     MTIDYEEGGFDYYDGAVSQQALNSKYGPGSYRFTLSSYITGDTRYDVPLASDDYPPAP
                     RCTNFDAAQRVDPTKDFTLRWATFTGEGEREVWIHITDSETGEFAFYAGPIDGAETAI
                     DIPAGKLTSDTTYRVWILFTRYTHLSYQTLPATYSGFDAYNSFPLKATSGGSAPDPAV
                     ITAYRVLENGDLELTADCTADHPLTVQGARALDAPWSDLQTTTPASSQVVLTVPKATL
                     GNRLFLRLVQE"
     gene            7755..8984
                     /locus_tag="PKK20_00455"
     CDS             7755..8984
                     /locus_tag="PKK20_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNU98393.1"
                     /translation="MAMRAWASQVFVWVFLGLHGSILASEWYAATNGTATAEGSRRAP
                     WDIESALAGRQPIAPGDTLWLRRGVYKRPFENVGMGWPVKLAGRAGAPVHVRSMPGER
                     VILDGGLNVQSPSSHLWIWDLEILVSEPRPAEPVPPDPTYRNLNRPWGGLNVYGGGDC
                     KFIHLIIHDNSQGVSWWAGSTNSELHGCIIYDNGWAGTQRGHGHAIYTQNRHGVKTIS
                     NNILTGGLGYSLHAYGSRQAFVDHCRVENNMAYDAGTFLIGGAQPSHGIRVLTNLFYG
                     MSVQLGYSAVTNVDCELRGNLFVNSRLTISRFARVVEQDNWLLGALETRPSGFRVVLE
                     PSKYDPQRAHLAVLNWDRTPSVAADVSMFLQPGDRFRVLEPTDFFGDPVLSGIAHGDP
                     LSLPTPTEFTVFVLLKR"
     gene            complement(8988..9818)
                     /locus_tag="PKK20_00460"
     CDS             complement(8988..9818)
                     /locus_tag="PKK20_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNU98394.1"
                     /translation="MSWPADYHMHTSLCRHAKGEPSDYARRAIERGLDEIGFSDHSPM
                     PRDDFDDWRMRLDQLSDYVARVDRARREAPSLTIKLALEVDFIPGQEEWVRDLAGRHP
                     WDYLIGSVHYVTESWDIDNPHRIALWKGRDLFEVWTAYFERLTQAAESGLFDIIGHAD
                     LCKKFGFRPDADCTPLYRRFLASARRAGVAIELNTAGLRKECREIYPSRALLELAHRE
                     GVPIAFGSDAHAPEEVGADFEEAVALARSVGYAHACRWTRRARTTVPLLAPGPAEIKS
                     "
     gene            complement(10049..11065)
                     /locus_tag="PKK20_00465"
     CDS             complement(10049..11065)
                     /locus_tag="PKK20_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNU98395.1"
                     /translation="MPHPVRCGNWKFSPRRIAGPLRAATLAAIPQDETGVFRIAAFYW
                     RGPAIRQTVDVRRRSLIELNTAVVLWAGTALFAKWIPLPAHQITGLRSVVAAGALLVL
                     LTARRDSLGVRGLRDLGLLIGSGVALAAHWITYFQSIQTSTVAIGILSVHAYPIMTAL
                     VEPLWFKEKLRAFDVGLAAVVGVGLIVLVPEFSPDSPILQGTLWGLLSAVLFTLRNLL
                     TRHLRSVYSGIHVTFWQLATSVAVLLPTTLAWGEPVSASSAYKLVLLGVLFTALPHTL
                     YTNALEHLSARSVGMLGTLLPVYGTVTAALLLGEVPSMRTIVGGAIVLGAVTIETGRA
                     LHTR"
     gene            11081..12211
                     /locus_tag="PKK20_00470"
     CDS             11081..12211
                     /locus_tag="PKK20_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98396.1"
                     /translation="MQTEALAWMPRRTMRPVVRAFFVSVVLHALLFGSIEVVNRYELW
                     RYSPLVLLAQALGVDVESTLAAQRASAPPAAENPALREADQEIPVLFVDVDPSQATTE
                     TPAKTPYYSSVSSLAGNRDTSRDTDTPAFDGTQDKVLKTMDSERAQVAQPLEPAPVAP
                     QQPQDGIVPEAIQPQAVTVAPAPLQPLPPPVAEPDPARPPGETLLAKANPVPAAPAVT
                     VPVPTPVEGARAARPRPRTVAAALAQRQLNPNSALVGEKMKQEGGVRRFSVSSSLDVQ
                     ATPLGSYDAKFIAAVQQCWYALLEQQGYSLDRMGKVVIDFRLTPDGRITDLKTVQSDV
                     GEIYTMICELAIHKPAPYEQWPTDVRRMVGAASRDVRFTFYY"
     gene            12221..12874
                     /locus_tag="PKK20_00475"
     CDS             12221..12874
                     /locus_tag="PKK20_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNU98397.1"
                     /translation="MWEFLLAGGPFMVVLVLTSIVGLTFIIERGLALRRSRVVPASIE
                     EAIETTGSKEDLQALRVLCQQKRSTLGRLVLLAIEHLPWPKPDNADCIQTRARNEIVQ
                     LERGLVVLEVVVGIAPLLGLVGTVHGLITLFADLGQTGLADNSVLAKGIAIALNTTLM
                     GLLIAIPTLVAWSYYTAKVQALAVEMETLLNDFLRRQYRHRTRIAPAKETAGSTRKA"
     gene            12883..13299
                     /locus_tag="PKK20_00480"
     CDS             12883..13299
                     /locus_tag="PKK20_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNU98398.1"
                     /translation="MRFFERKRRQPPAIIIVSLIDILIVLLIFMMVTTTFKRHPAVRL
                     TLPESSQPREGASEASLIVTVANEPPFFFLGEQRITLDALRAELAARVAREPDLALSI
                     RGDTTATWGHIVEVMDAAKAARVKSISAQVKTPGGN"
     gene            complement(13466..17896)
                     /locus_tag="PKK20_00485"
     CDS             complement(13466..17896)
                     /locus_tag="PKK20_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNU98399.1"
                     /translation="MSATHAPPPGPGQRPPPGAPPAPPQAGSASSLRGSEPASEGGGA
                     PTRVLGIDLGGETIKIVELTRVDGQWRWTRRVHFEHAKQPGPRLVAALADFDWPTLSG
                     AASSGRLARLVRLPRVPTKQAQARAYRFLFGNDPATVISIGSHGFSVLELRDKGIEIF
                     RENSRCSQGTGNFLRQLTERFGLDVGAASALCADVSDPAPLSGRCPVILKTDMTHLAN
                     KGESRGRILAGLFDAVGANVLTLLKPRLSPSPVALIGGVSRSLRVQRTVARFLSENGF
                     QSRTVAPEDALFFEALGSALVAAERQAPLPTLSEVIAGETRSRLERLPALAQSLPLVR
                     RMVSPVAPRIEGLERRLILGLDIGSTGSKLVALDEVAGEALWDCYRRTGGDPVGAAQA
                     LIRAFVDGPAGRFPVLGLGVTGSGREIVGSLCRTCYGSDAVLIINEIVAHAEGALHFD
                     ARVDTIFEIGGQDAKYIRLDEGRVIDCAMNEACSAGTGSFIEEQGRKFAGLDDVAQLG
                     REALDASHGVSLGQHCSVFMAEVIDEAVAAGVDQSAILAGLYDSIIQNYLNRVKGSRS
                     VGRVIFCQGMPFASDALAAAVARQTGGEIIIPPNPGTVGALGIALLARGTISLDRADG
                     LDLDRFLSARVERKDTFVCQATVGCGASGNHCRIDSLTTVVLGERRVFRWGGACALYD
                     KGTRRIKLPDRSPDPFREREALVADLMHRLDAPPPGNGTRRRIALTDEFALKGLLPFF
                     SVFLRTLGFDLVFHTGAGRAALKRGIQEANVPFCAPMQLYCGLASQLAAEPADYVFLP
                     MIRHLPRVDSEPFAKTCPILQASPDMIRWGLDGGDRSRVLSPVINVGPANLDSPEFVE
                     SCRRLAASLGIGGRRWRAAWEAAREAQLDFDRRCFEIGGRVLAFCAERDVLPIVVLGR
                     PYTIYNTVLNSNVPALLREQGALAIPLDCYPVDRAVPVFSEMYWAYGQRVLRAAHQIR
                     RTPGVYGLYASNYSCGPDSFNLHFCAHVMEGKPFAVIETDGHSGDAGTKTRIEAFLHC
                     VREDRSRARGGSAPSDFGGLARAQTEWRGIQDRGEVLLIPRMSGVAEMVAACFRGLGM
                     RAECLPEPDAEALRLGRRQTSGKECLPMCITLGSLLQRSARLREPGERLALLMPCAAG
                     PCRFGAYSLLHQIVVGRLGLGDRVRLWSPKEADYFAGLPPGFSLLLFSGVMAFDVLHE
                     ALLETRPLECRPGAAAAIHTAAQARLERLLEEHARTDLSARAALHAVASGHLFGLRDL
                     VARTAADLAALRADRPMPTVLVVGEIYVRLDPFANSYLIRELEARGLRVRLAAVNEWL
                     EYVEHFHQINASALDLSPRLRVFLQNRIQNILYRAAGRAFQWPERISVRDCLDAALPY
                     LRPALEGEAVLTLGGPIRAWRLGQIDAVVSAGPLECMPNKIAEAQFFHVAEQEGLLSL
                     TLSLNGEPVAPDVLDNFAFEVHERFARRASERTPSMRPSW"
     gene            complement(18263..20770)
                     /locus_tag="PKK20_00490"
     CDS             complement(18263..20770)
                     /locus_tag="PKK20_00490"
                     /inference="COORDINATES: protein motif:HMM:NF026756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4623 domain-containing protein"
                     /protein_id="HNU98400.1"
                     /translation="MKHSNEPNPCPVVRFLLLLMLGLTLGFSARAQSYQLTPVWSLAP
                     GDVAFLDTTHLTRGLAYNPTTDHLLVPSRTGSAAVHVLDAGTGGELGTLPFDTSVITG
                     GTFPVNMVAITDDGVIYVGNLTVDTTGASGPFRLYRWADEAAQPELVYSGDPSEADGV
                     ANNRRFGDSLVLRGTGSNTQILLGTRDKNVALLTTADGMTFTAQKIVTDCAADDTRWG
                     LAWGLGNTFWAKQAGGNLKQFTLDLTAGTAAVTTTVTGIVGAPLDLDPARALLGIVEA
                     GSADTANHRLRLYDISNPAAPQLQDAAKDFPGGYSNGNLTGAVALHAGRLFALESNNG
                     MMAFDLIEVFLPPGIATQPANATVWQGAQTYTFSVGVSGTQPFTYQWRFNGEEIAGAT
                     SPTLTLSNIASASQGAYSVVVSNLSGSATSAEATLTVLPSNASPQVENIWNVLPGTRP
                     YLTSGYKEYGLAINPLTTNVIVVTRLNPTNMIAVIDMQTGEHKHYIDYSGLTLYGGMN
                     KVDVADDGVIYICNVTGNTDTTPFTIYGIGDDSPYTPDQWLAFSGDPGNGITAPTDGW
                     GANIDVRGGGLETEILVGAGKWSPTPLQARTVAILKPDPNYEFISTTITVPDAPPNIF
                     RFGLAWGPGNNTFWVKAVGSLYLIEFDLGTGLGAVKATYPTEGDRSVPASVTGIKYDA
                     ATGLLAGLRNGSPPSPVSVPVYDVRDLAAGPLWVDNELFTTYNPDIEFQGNVDFGAGY
                     LVALGVNNGLKAFLIDPTYVPPAESFKILSVTSAAGGIALEWQSKVGASYQVQTASAV
                     NGPWQDLGSPITAAGNTASFVDQTPAAAQRFYRVQIR"
     gene            21143..22042
                     /locus_tag="PKK20_00495"
     CDS             21143..22042
                     /locus_tag="PKK20_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU98401.1"
                     /translation="MKMNAYEKAAVADGAHARPCGRQCGFTLIELLVVIAIIAILAGM
                     LLPALSKAKVKAQAIACLNNVKQLQLAYELYATDNGDTLMDNSVTGVASPGDKAWIKG
                     NVQEYAADYLDHPRFGVLFLYNSSVEIYRCPSSRAYIQGVGRGSGARVTHNRSYAVSV
                     WLGNNLDLGADRTGLIAHRFSAVRNPSSTSVFIEENAISIDNGAIGFNRPIPGNAGSD
                     PGGVWNLPANRHNNGCSFSFLDGHGETFRWRGPRIKELNMQYSADHSRSQRPSAGVNP
                     IHSLPWESKDPDYQRLADTAPLL"
BASE COUNT         3957 a         6959 c         7357 g         3960 t
ORIGIN      
        1 gcggaacaca gattcccgaa gaggacaagt tcttcctcag gaactggccc aaggagaatc
       61 tggcccttgt tcttgagaaa ggaaacgggc ggatcggcac tgaacagttc gtggtttgtc
      121 gagatcatcg cacgcctact acgccccaaa ccgcgccaat tgacaagcgt tatttacaac
      181 ctcaacaaca gcaacccttc aggaatttaa atgaaatgac cgtggccctc caccaaggct
      241 cttcgcgcaa ggctcgacga agccgccccg cccctttaga ctccgccgct ggacgaacaa
      301 gactcagcgg tttgtgcaag aaataggaat tgagttttgc acagatcctt tgggctcatg
      361 aatctgccga acaaactcac agtgtcgcgt tttgcgctca cggtggtctt tgttttggcg
      421 gtgttgtcgc gcgggcccgt caacgacacg ctggccctgg tgtttttctg cgccgcaggc
      481 atcacggact acctggacgg acggatcgcc cgtcggcgcg gtctgatcac caacttcggc
      541 atcctcatgg acccgctggc cgacaaggtt ctgatctgca cggcgttcat catgctgctc
      601 gaacgcggcc ggatgaatcc gtcggctccg gtgacggtcg aggggtggat ggtggtgctg
      661 atcgtggcgc gcgaactgac gatcaccggc ctgcgactgc tggcggcgtc caagcgcatc
      721 gtgctggcgg cggagggtta cggtaagcac aagaccgtct cgcagatcgt ggcgatcatt
      781 tcattgctcg tgctggacgc gcacgtcgag tggtggtcct ggcttcgcgg gctgttcgag
      841 tggtgggtgc ctgccttcac ccaactcgcc ctctatatga cagttgtgct gaccgtgacc
      901 tccggtgctc tctatctctg gcgaaaccgg gcgctctatc tcgatgacat gtgagcgcaa
      961 gtggagggac gcccttgtcc tttggctagc tcgaggcttc ggtgtcgggc gtgtgccggt
     1021 cgctccgggc acgttcggat cggtggtcgg cctggcctgg acgcttgtgt tgctcgcgac
     1081 acggagcgtc tggtggtacg gactgggcgt tgcggcgggg ctggctctgt cggtcgttgt
     1141 gtgtgggctg gctgagcggc ttcttcggga acgcgatccg ggctcggtcg tgctcgatga
     1201 gatcacggcg cttcccttgt gtttcgccgg atggatcggt catcatctct gggtgacggg
     1261 cgcgatgccc gcgccggagg tcttggtgac ggggcgcgga tggctggtct tggcagccgg
     1321 ttttgttgcc ttccgtgtgt tcgatgtcct caagccctgg cccatccgtc aaagccaggc
     1381 gatgccggga gggtggggtg tcacgatcga cgatgtcctg gccggacttg catcgaacat
     1441 catctggatg ccctgtgcct ggctcagcta gcccgcatat tcacaccccg tctgtgcatg
     1501 gggcactcgg acttcgcaag aggacttcaa tgcgggctag accggcgccg gccagtcgtc
     1561 gcccaatgcg ctcggggcct gctggcgcca ggtgccacgg gtgaagtcgg gaaccgtcac
     1621 gggcgcgctg cgtcgagcca cggaggcgat gctcagcggc acaatgctgc tccaggcggc
     1681 ggcgtcataa acggtcatgt cgagcgggag tcctcgccgc aggcattgga tgaggcgcca
     1741 gttcatgacg taatccatgc cgccatgtcc accggattgc cgggcctggg cattgaggct
     1801 tttccagaag ggatgaccgt gcttgtcgtg gtacggctgc agttctgtgg tccaatcctc
     1861 gccctgacca tccagatgca gtcggggcgg atagtcacaa aacgtgcccg tggtgcccgc
     1921 gatgagattg atgcggctgt aggggcgcga ctgagccagc gagtactgaa tcatgatggt
     1981 gcgccccaag gcggtcttca ccagcgtggt gttgatgtcg ccgcaggcga acgccctgcc
     2041 gcggcttgtg gacgcgccgg accgggcttc gagacgccgg ctcagcgcga gttcgagcga
     2101 actcatcgat accaggtaat caaacgtatc gccggcatga atacccatgt attccgcgac
     2161 ggggccgagt ccgtgggtcg ggtagaggtt gccgtcgagc gtggcgacga accgccggcg
     2221 ccagttggcc gggctgtcct cgcgcagcag ccagtcgcgc gcctcgtgga gatagccggc
     2281 ctcgccgtgc gtcaactcgc ccaagacgcc ctggcgcacc atgcgcagca ccatgagttc
     2341 gatctcgccg tagcagcagt tctcgagcat gaggcagtgg cggcgcgtct gctcggctgt
     2401 atccaccaaa cgccagcaat cgtcgagtgt catggcggcc ggcacctcga ttgctgcatg
     2461 cttgccatgg cgcatggcgc tgacggccat cgggacatgc cagtcccagg gcgtggcgat
     2521 gtagaccaga tcgagatcgt cgcgttggca gaggcgctca aagtccatct cgccctccga
     2581 atagccttcc ggctccggct ggttcttcgc cgcaacacgc cgtcgggcgg cctcgacccg
     2641 ctccggcaca atgtcgcaca cggccttgat ctggagtccg tcgagttcga gcaggttgcc
     2701 cagcaaactc gaaccgcggc tcccgacacc gatgaagccg atgcggacca tgtcgagggc
     2761 cgggtgacgc aatccaaaga cccgctgctg cgccggctga ggcgttggcg gaggcgggtc
     2821 cgcggcggcg gcggcagccg tctttgcgat cagggcgcct ccggcgccgg cagcgcccgc
     2881 gacggcgagg aaatcacggc gcgacaggga agcgccgtga gcggatgtga tcttatgtgg
     2941 catcctggtg tcctttgaaa tcggttcgag tcgtcgcgtt ccatccggcg tgatcacccg
     3001 cgaatcgctc ccgccaggcg ggtgcggacc gcccggcagc cggggcccgg cgcgtgtcaa
     3061 gagtcgccag tgcggtcgag acttgtcgcg acgcgccgga aatggtggcc gatgatggcc
     3121 atctcgatcg cctggcgaaa ctgatgtggt cgcaacaaca gcgtggtcca gaggagccgc
     3181 cagtaagcgc gccggccgcg gtgggcgacg ccgagaaccc agaacgattt gagaaaggcg
     3241 cgcacatcgt cgggcgaaag acgcagtgct ggtccgcgcc gcggttggtg ttggcgcagg
     3301 aagttgcgga tgcgctgata gtaggcgcgg ggttcgtaga gccgattcat caggtcgcga
     3361 taaccggaaa ggaggaaatc gcgattgagt ttcggacgga aattcaactc ggcttgggtg
     3421 ttgttgccgc agctctcgcg ctcgaggcgg ccttcgcgct tgagccgttc gtagaggcgc
     3481 gtgcgcggca acgccgtgag cagacccacc atcgcggtca ccaccccgga tcgctggatg
     3541 aattcgaact ggcgcttgaa aatgtccgcc gggtcgctgt cgaatccgac gatgaacccg
     3601 cccataactt cgaggccggc ctgttgcatc gtctgcacgg ccgacatcag gtcgcgcccg
     3661 cggttctgca tcttgtggca ctcggccagc gactcggcgg agggcgtctc gattccgacg
     3721 aagaccttgg tgaagccggc ctggaccatc aaccgcagca actccggatc gtcggccagg
     3781 ttgaccgagg cctcggtcag gaagcccccc ggcgcgcccg tgccggtccg ccactcgatc
     3841 aacgtccgga gcaaggcgcg ggcgcgtcgc ttgtcgccga tgaaattgtc atcgaccacg
     3901 aacaccatgt ctttccagcc ggcccggcgc agcgtttcga gttccgccac caactgcgcc
     3961 ggcgtcttgg tccgcggcac gcggccgttc atgacgatga tgtcgcagaa ttcgcagtcg
     4021 aacgggcagc cgcgcgagaa ctggaccgcc atcgtgacat aattgcgcag gtcgatgagg
     4081 tcccagcgcg gaatcggcgt gcgcgtgaca tcggggtatc ctgaagcccg gtacacgggc
     4141 ttgagagtgc cctgttccag gtcccgcacc agctccggca tgacgtcctc agcctcgccc
     4201 aggacgacat gcggcacttc ggagaactcc tcgtggccgg tggtgaaaag cgggccgcct
     4261 ccgatgaccg gcttggccgc cgcggcgcaa cgggccacca catcgagcac tgacgcccgc
     4321 tggatgcgca tcgcgctgag catcacgtag tcggcccacc gcagatgctc atcccgcaga
     4381 cgctcgacgt tcaaatccac cacccgcagc gaccaatccg acggcagcat cgctgcgacg
     4441 gtcaacaggc ccaagggggg aaacgccgcg cgtttggaca ccatccgaac gacatgacga
     4501 aaactccaaa aggtatcggg tgtctgggga ctgactaaca gaatcttcat gcagtgtgtg
     4561 ccgcctcgag gaccgtccgc gttcatcggc ggtcagcctc tctgtcggtg tggcgtacgt
     4621 tcaatgaaca agttcaagga aaccgaactt tgccggcgtg tgcgcggtgg ccgtggcggt
     4681 cgggctccat gccagaaagg cccgttccgt ggcggcgtgg cgatagagat tgaggcgcca
     4741 gcgcgtgcct ggctcgggct tggacggact gagagccgca agcggaatcc ggaattcggt
     4801 ggtccagacc ttggtcctgc ggttcaggcg gacggccgat tcgaaaccgc tgctccagtc
     4861 gaagtccttg tcctgtcccc ggatggccag gtccaaccgc tcgccggtcg gggccacctc
     4921 gaattcggta tagtgatcgg ggcgggtcgg atcggttccg atgaacgcct cgacaacgtc
     4981 gcgttcccat aggccgaggc gttccttgtc tttgaggacg ggcgcgaatg tggcaagggt
     5041 ctcgtaaggc gcctcgaacc cgaaatagag atgcgagtcg gaccagagga tacgggccgt
     5101 cgtggccaga tcgggtcgtg cggagccatc cttcaggagg tactcgagac gcgcgggtga
     5161 tgcggcccgc caagccggtt cgtccaggcg cccgttcggt ttgaaatccc ggtcgatctt
     5221 ctttgcctga accaccggca aaggcagcga tcggaccagc aaccgccggg cgttgtcgaa
     5281 gtagatcttc cgcagcacgc tggccggcag gtctatggcg tcgatcttcc agtctccctg
     5341 aatgggcgtc atgtgctcga attggcggtc ggaggtctcc agccaccgcc agtgcttttc
     5401 gtagaactcg atggcatcct cgtcggtcgg cggcgggccg ctgccgcccg acccgagcgt
     5461 gaggtttgcg tacaccatga aatccgtgcc gaacagaatg cggtcttggt gtttgacaaa
     5521 gagccgatgc acgcgcgccg gatcgtggcg tccgatctcc gggatgcgcg ccgcgaggtc
     5581 ggcgtgcatg ttggggcgct tgtccagggc ctgctcgacc caatcgatgt cctcggcgtt
     5641 attggcgaaa tgcacgcaga caaacgtggt ttgcgggtgg cgctcgatca cacggtccag
     5701 cgccgtcaga agctcgtgga acggcgggta tttcgccggg tcgccaaacc accattgcgg
     5761 gtgatccttg agttcgcgcc aacgctcgtt ttcggcgttg tagggcttcc agaaggcctg
     5821 cggatccgcc acgtgaatcg acacgggcat tcccaactcg ccgcagcgcc gccagaccgg
     5881 atcgagcttc ggatcgtcca cgcggatcaa tcgcccggcc ttgtctttca gataaaggcc
     5941 cagccgcttg tattccttca aacccgccgc cccgagccga tacccctcct cgagctgttg
     6001 cacggcacgt tccgaaaagt cgggctcgtc ccaaccggcg aagtccagac tcatgtaata
     6061 caggaagcgg cccgggaaga ggcgatcggc cagctccttg ttgcgctcga atgcggattt
     6121 ctcgcccggc ttgtgcgtga cgaagccgcc gctcagattc acaaccaacc caacaccgac
     6181 ggtgtccatc aggcgcatgt cccgtcgcag cgccgtttcg ctggaatcga cgtgttgatg
     6241 gagatcgatg agccgatgct cggcccgcca cgcggcggcg gtcttccggg tggcgtcatc
     6301 ggccagtccg gcgattggaa caagcaagac ggccgccaga agccagggtc gcggcgacca
     6361 tcgggcgcgc ggggtcgagg cgcaggggat cggcagacgg cggttcatgg cggcatccaa
     6421 aggagatcga aagtgagtgc tcattcgaag acaccgcaat taaagccctt ctgtggtccg
     6481 cgcgcaacca gtttgggcgg ggtctactcc tggacgaggc gtaggaagag gcgattgccg
     6541 agggtcgcct tggggacggt cagcacgacc tgcgagcttg ccggcgtggt ggtctggagg
     6601 tcagaccacg gggcgtcgag cgctcgagcg ccctggacgg tgagggggtg atcggcggtg
     6661 cagtccgcgg tcagttcaag atcgccgttt tcgagcacgc gataggcggt gatgactgcc
     6721 ggatcgggcg ccgatccgcc cgaggtcgcc ttgagcggga aactgttgta agcgtcgaag
     6781 cccgagtagg tcgcgggtag cgtctggtag gagaggtgcg tgtagcgtgt gaacaaaatc
     6841 cagaccctgt aggttgtgtc cgatgtcagc ttcccggccg ggatgtcgat ggcggtctcc
     6901 gcgccgtcga tcggtccggc atagaacgca aactccccag tctctgaatc cgtgatgtgg
     6961 atccagacct cgcgctcgcc ttcgccggtg aaggtcgccc aacgcagcgt gaaatccttg
     7021 gtgggatcga ccctctgggc ggcatcgaag ttggtgcatc gaggcgcggg ggggtaatcg
     7081 tccgaggcca ggggcacgtc gtatcgggtg tctccggtga tgtagctcga caacgtgaaa
     7141 cggtagctgc cggggccgta cttcgagttc aacgcctgct ggctcaccgc cccgtcgtag
     7201 taatcgaaac cgccctcctc atagtcgatt gtcatgccat gcgtttggct gctgggcgtg
     7261 cgaactgtca tgcccgaaat ccaccaatac cattccgggt cgctgagcgt ctcgtcggtc
     7321 agataaacat cggccgtgat gtcatagggt tcgtccgcac tcggaatcgg cacgggatcg
     7381 ccggcgtcgt tctgttcgta ccagacggtc ttgaagagga aaattgcgcg gacgtcctcc
     7441 gcgtgggccg ccggcgccag gagcatggcc aagcccaacc aggcgccgag gaaagaagtg
     7501 aaggggcgtt tcatggcaaa ccaagtgaag gcctgcttca tccggcagtc aagccattgc
     7561 attcttgatc ttgtcgatgt cgttgcacgt gctgctcgcc acggatcgat gaagtgcgcg
     7621 atggagtgcg cgatgatgta caatccgaat agtgcccaaa ctcctgggcc gcgtgcccca
     7681 cgcggcgcaa gaacctacac ctattcggac atcgcgtctc gtcttgcggc ggattccatc
     7741 gccaggcaga ctcgatggcg atgcgtgcct gggccagtca ggttttcgtt tgggtcttcc
     7801 tcggattaca cgggagcatc ctggcttccg agtggtacgc ggccacgaac gggaccgcca
     7861 cagccgaagg gtcccgtcgt gcgccctggg acatcgagtc ggctttggcc gggcggcagc
     7921 cgatcgcccc gggcgacacc ctctggctgc gtcggggcgt ttacaaacgc ccgttcgaga
     7981 acgtcggcat gggctggccg gtcaagctgg ccgggcgcgc cggcgccccc gtccacgtcc
     8041 ggtcaatgcc cggcgagcgg gtgatcctcg atggagggct caacgtgcag tccccttcga
     8101 gtcatctctg gatttgggat ctggaaatcc tggtgtccga gccgcgccct gcggagccgg
     8161 ttcctccgga cccgacatac cggaatctga accggccgtg gggtgggctg aacgtgtacg
     8221 gcggcggcga ctgcaagttc atccacttga tcattcacga caactctcaa ggcgtgagct
     8281 ggtgggcggg gtcgaccaac agtgaactgc acgggtgcat catctatgac aatggctggg
     8341 cgggcactca acgcggtcat ggacacgcca tttacaccca aaaccggcac ggcgtgaaaa
     8401 cgatttcgaa caacatcctc accggcgggc tcggctacag cctgcatgcg tacgggtctc
     8461 gtcaggcctt cgtcgaccat tgccgtgtcg agaacaacat ggcctacgac gccggcacct
     8521 tcctgatcgg cggggcccag ccgagccatg gcattcgcgt gctgacgaac cttttctatg
     8581 ggatgtccgt ccagctcggt tactccgccg tcaccaacgt cgattgcgag ttgcgcggca
     8641 atctcttcgt caattcccgg ctcacaatca gccgcttcgc gcgcgtcgtc gagcaggaca
     8701 actggcttct gggcgcgctc gagactcgcc cgtccggatt tcgagttgtc ctcgagccca
     8761 gcaaatacga tccccaacgc gcccacctgg cggtgctcaa ttgggatcgc acgccgtccg
     8821 tggcggccga tgtctcgatg ttccttcagc ccggcgaccg gttccgcgtg ctcgagccga
     8881 cggatttctt cggcgacccg gtgctgagcg ggattgccca cggcgaccct ctgtcgcttc
     8941 cgacccccac cgaattcact gtcttcgttc tcctcaaacg ctgattctta gcttttgatt
     9001 tccgccgggc cgggcgccag gagcgggaca gtcgtgcgcg ctcgccgagt ccagcggcag
     9061 gcatgggcat agcccaccga acgggcgagg gccaccgcct cttcgaaatc ggcgccgact
     9121 tcctcaggcg catgggcgtc cgatccgaac gcgatgggaa cgccctcccg gtgcgccagt
     9181 tcgagcaagg cgcggctcgg gtaaatctcc cggcattcct tgcgcagacc ggcggtgttc
     9241 agttcgatcg cgacgccggc acgcctcgcc gaggcgagga accgccggta gagcggcgtg
     9301 cagtcggcgt ccgggcgaaa tccgaatttc ttgcagaggt cggcgtgccc gatgatgtcg
     9361 aacaggcccg actcggcagc ctgcgtcagc cgctcgaagt aggcggtcca cacctcgaac
     9421 agatcgcgcc ccttccagag ggcgatgcgg tgggggttat cgatgtccca cgactcggtc
     9481 acataatgca ccgagccgat gagataatcc caggggtgac gccctgcaag gtcgcgcacc
     9541 cattcctcct gcccaggaat gaaatccact tcgagcgcga gcttgatggt cagggagggc
     9601 gcctcgcggc gcgcccgatc gacccgcgcg acgtagtccg acagttggtc caggcgcatg
     9661 cgccagtcgt cgaaatcgtc gcggggcatt gggctgtggt cggagaatcc aatttcatcc
     9721 agaccgcgct cgatggcgcg gcgggcgtaa tcggacggtt cacccttggc atgccggcag
     9781 agcgaggtgt gcatgtggta atcggcgggc catgacatcg gaccctttgt atcgccagtc
     9841 gtcgtccatg ggaagcgttg acattgaacg gttgaatgcg cacggggacc atgaaccgat
     9901 ttgtagccgc cgacgtgagt cggcgctgat ttctgctttt gacggagcga gatggcgcgg
     9961 actcacgtcc gcggctacgg aaccacggtt catgggaagc gttgacattg aacggttgaa
    10021 tgcgcacgga gcctgtgaac gacccgggct accgcgtatg gagggcgcgt cccgtctcga
    10081 tggtcacggc gcccaggacg atcgcgccgc ccacgatggt gcgcatcgac ggcacctcgc
    10141 ccaggagcag tgccgcggtg acggtgccgt agacgggcag cagtgttccg agcattccga
    10201 ccgaccgagc gctgagatgt tcaagcgcat tggtgtacag ggtgtgcggc agggccgtga
    10261 atagcacgcc cagcagcacc agtttgtacg cggacgacgc gctcacgggc tcgccccagg
    10321 caagtgtcgt cggcaacagg accgccaccg aggtcgccaa ttgccagaag gtcacgtgga
    10381 ttccgctgta caccgaacgc agatgccgag tcaggagatt ccgcaaagtg aacagaaccg
    10441 cggaaagcag cccccagagc gtgccctgga gaatggggct gtccggggag aactcgggca
    10501 ccagaactat cagacccacc ccgaccaccg cggccaggcc gacatcgaac gcgcggagtt
    10561 tctccttgaa ccacaacggc tcgaccagag cggtcatgat gggataggcg tgcacggaca
    10621 gaatgccgat ggcgacggtc gacgtctgga tcgactggaa gtaggtgatc caatgggccg
    10681 ccaacgccac tcccgagccg atcaacaacc caaggtcgcg cagtccgcgc acgccgagcg
    10741 aatcccgccg ggctgtcagc aggaccaaca acgccccggc cgccacgacc gaccgcagcc
    10801 cggtgatctg atgcgcgggc agtggaatcc acttggcaaa gagcgccgtg cccgcccaca
    10861 acacgacggc ggtgttcagt tcgatcaggc ttctccgtct gacgtccacg gtttggcgaa
    10921 tggccggccc gcgccagtag aaggctgcaa tccgaaaaac tccagtttca tcttgcggta
    10981 tggcggcgag cgtggcggct cgcaatgggc cggcgattcg ccgtggtgag aacttccagt
    11041 tgccgcatcg gacggggtgc ggcattctgt aatccgcaca atgcagactg aagcgcttgc
    11101 ctggatgccg cgccggacga tgcgtccggt cgtccgggca ttcttcgtgt ccgtggtgct
    11161 gcacgcgctt ctgtttggct cgatcgaggt tgtgaaccgc tacgagttgt ggcgatacag
    11221 cccgctggtg ctcctggccc aggcgctggg cgtcgacgtc gaatcgaccc tggcggccca
    11281 acgcgcctcg gcgcctccgg ctgccgagaa tcccgcgctc cgggaggcgg accaggaaat
    11341 cccggtgttg tttgtggatg tcgatccgag ccaggccacg accgaaacgc ctgcaaagac
    11401 cccgtattat tcgtcggtca gttcgttggc cggcaatcgc gacaccagcc gcgacacgga
    11461 taccccggcg tttgacggca cccaggacaa ggtgctcaag accatggaca gcgagcgcgc
    11521 gcaggttgca caaccgctgg agccggctcc cgtggccccg caacagcccc aggacggcat
    11581 cgtccccgag gccatccaac cccaggccgt cactgtcgcg cccgcaccgc ttcaaccttt
    11641 gcccccgccc gtggcggagc ccgatccggc acggcctccg ggcgagacgc tgctggccaa
    11701 ggcgaatccg gtccccgccg cgccggcggt gacggtgccc gtgccgacgc cggtcgaggg
    11761 ggcgcgggca gcccgtccgc gtccccgcac ggtggcggcg gcactggcgc aacgacagtt
    11821 gaatccgaac agcgctctgg tgggcgagaa gatgaagcag gaaggggggg tgcggaggtt
    11881 ctccgtgtcc tcgtctctcg atgtgcaggc gacgcccttg gggagctacg atgccaagtt
    11941 catcgccgcc gtccagcaat gctggtacgc tctgctcgaa cagcagggtt acagcctcga
    12001 tcgcatgggc aaagtcgtga tcgatttccg gctgactccc gacggacgca tcaccgactt
    12061 gaagaccgtc caatcggacg tcggcgagat ctacaccatg atctgcgagt tggcgatcca
    12121 caaaccggcg ccgtacgagc aatggccgac agacgtgcgc cggatggtgg gggcggcctc
    12181 ccgcgatgtg cggttcacgt tttactactg agacgaaact atgtgggagt tcctcctggc
    12241 tggcgggcca ttcatggtgg ttttggtgtt gacgtcgatt gtcggtctga ccttcatcat
    12301 cgagcgcggt ctggctttgc ggcggagcag ggtggttccg gcgagcatcg aggaagcgat
    12361 cgaaacgacg gggagcaagg aagacctcca ggccttgcgc gtgctttgcc agcagaagcg
    12421 ctcgacactc gggcgactgg tcctcctcgc catcgagcac ctcccctggc caaaacccga
    12481 caatgccgat tgcattcaga cgcgcgcccg aaacgaaatc gtccaactcg aacgcggcct
    12541 ggtggtgctc gaggttgtgg tcggtatcgc tccgcttctc ggcctggtgg gaaccgtgca
    12601 cggactgatc acgctctttg ccgacctggg ccagaccggc ctggccgaca actcggtgct
    12661 cgccaagggc atcgccatcg ccctcaatac gacgctcatg ggcctgctca tcgccatccc
    12721 cacgctcgtc gcctggagct actataccgc caaagtccag gccctcgccg tcgagatgga
    12781 aacgctgctc aacgatttcc tgcggcggca gtaccggcat cggacacgca ttgcgcccgc
    12841 gaaggaaacc gccggttcga cacgcaaagc ctgaccccag ccatgcggtt cttcgagcgc
    12901 aaacgccgcc agccccctgc catcatcatc gtgtcgctca tcgacatcct gatcgtcctt
    12961 ctgatcttca tgatggtcac cacgaccttc aaacgccatc cggcggtcag gctcaccctg
    13021 cccgaatcga gtcagccgcg cgaaggcgcc tccgaagcga gcctgattgt cacggtggcc
    13081 aatgagccgc ctttcttctt tctgggagaa cagcgcatca cgctcgatgc tctgcgcgcc
    13141 gaactggccg cgcgcgtcgc ccgcgaaccc gacctggccc tgagcattcg cggcgacacg
    13201 accgcgacgt ggggtcacat cgtcgaggtc atggacgccg ccaaggccgc gcgcgtcaaa
    13261 agcatcagcg cccaggtgaa gacgcctggg ggcaattagg gtctgtgcaa aaattacttt
    13321 cggttttgcc ggaggggatt tggctcgctg gcgaggcgcg acgaaggagc atggcgttga
    13381 cctctgtggc ttgtgccgtc gaacatcatt tgcgaacgga ccttgaaggc ttttcggaac
    13441 cgcgagcatc cgtattatgc cgcgctcacc aagacggacg catcgacggc gttcgttccg
    13501 aggcacggcg ggcgaaacgc tcgtggacct cgaatgcgaa gttgtccagc acgtctggcg
    13561 ccaccggttc gccgttgagc gagagggtca ggctcaagag accctcctgt tcggccacgt
    13621 ggaaaaactg ggcttcggcg atcttgttgg gcatgcattc gagaggaccc gcgctgacca
    13681 ccgcatcgat ctgccccaac cgccaggccc ggattggccc gccgagcgta agcaccgcct
    13741 cgccctcgag cgccggacgc aaatagggca gcgcggcgtc cagacagtcg cgcaccgaaa
    13801 tccgctccgg ccactggaag gcgcgtccgg ccgcccggta caggatgttc tggatgcggt
    13861 tttggaggaa gaccctcagg cgcggcgaaa ggtcgagagc cgaggcgttt atctgatgaa
    13921 agtgctcgac atattcgagc cactcgttga cggcagccag tcgcactcgc agtccgcgcg
    13981 cctccaattc ccgtatgagg taactgttcg cgaacggatc cagtcgcacg taaatctcgc
    14041 ccacaaccag gacggttggc atggggcgat cggcgcgcag cgcggcaagg tccgccgccg
    14101 tccgggcgac cagatcgcgc aggccgaaga gatggccgct ggccaccgcg tgcagcgcgg
    14161 cccgtgccga cagatcggtg cgggcgtgct cttcgagcag gcgctcgagt cgggcctgag
    14221 cggcggtgtg gatcgcggcg gccgcgcccg gccggcattc gagcggtctg gtttcgagca
    14281 aggcctcgtg caggacgtcg aaggccatca cccccgaaaa gagcaggaga ctgaaccccg
    14341 gcgggagccc ggcgaagtag tcggcttcct tgggcgacca gaggcgcacc cggtctccca
    14401 ggcccaagcg gccgacaacg atctggtgca gcaggctgta ggcgccgaaa cggcaggggc
    14461 cggccgcgca aggcatcagc agcgcaagcc gctcgccggg ctcgcggaga cgggcggacc
    14521 gctggagcag gctgcccagg gtgatgcaca tgggcagaca ctccttgcct gaggtctggc
    14581 ggcgccccag ccgcaaagcc tccgcgtccg gctcgggcag gcattccgca cgcatcccca
    14641 aaccccgaaa gcaggccgcg accatctcgg cgactccgct catgcgcggg atcagcagca
    14701 cctcgcccct gtcctgaatc ccccgccact cggtctgggc gcgggccagg ccgccaaaat
    14761 cactcggggc ggatccgccg cgcgcgcggc tgcggtcttc gcggacacaa tggaggaagg
    14821 cctcgatgcg cgtcttcgtg ccggcgtcgc cggaatgccc atcggtctcg atgacagcaa
    14881 acggcttgcc ctccatgacg tgcgcgcaga agtgcaggtt gaaactgtcg ggtccgcacg
    14941 agtaattgct ggcgtagagg ccgtagacgc cgggcgtgcg gcgaatctga tgcgcggctc
    15001 gcagcacgcg ctgcccatac gcccagtaca tctcgctgaa aaccggcacc gcccgatcca
    15061 ccgggtagca atcgagcggg atcgccaagg cgccttgctc gcgcagcagg gccggcacat
    15121 tcgaattgag caccgtgttg tagattgtgt acggacgccc cagcacgacg atcggcagca
    15181 cgtcccgctc ggcacagaac gccaagaccc ggcccccgat ctcgaaacag cggcggtcaa
    15241 aatcaagctg agcctcgcgc gcagcttccc aggccgcacg ccaccggcgg cccccgattc
    15301 ccaacgacgc cgccagacgc cgacagctct ctacgaactc gggggaatcg agattggccg
    15361 gcccgacgtt aatcaccggc gacagcaccc tcgagcggtc cccgccgtcc aatccccacc
    15421 ggatcatgtc gggactggcc tggaggatcg ggcaggtctt ggcgaacggc tcgctgtcta
    15481 cccgcggcag atgcctgatc atcggcagaa agacatagtc cgcaggctcc gccgcgagtt
    15541 ggcttgcgag cccgcagtac aattgcatcg gagcgcagaa cggcacattg gcctcttgaa
    15601 tgccccgctt gagagcggcc cgaccggcgc ccgtgtggaa gacgagatcg aagccgagcg
    15661 tccgcagaaa gacggagaaa aacggcagca gccctttgag ggcgaactcg tcggtcaagg
    15721 caatgcgccg gcgggttccg ttgccggggg ggggcgcatc gagacggtgc attagatccg
    15781 ccaccagtgc ctcgcgttcc cgaaacggat cgggcgaacg gtccggtagt ttgatccggc
    15841 gcgtcccttt gtcatagaga gcgcatgcgc cgccccagcg gaacacacgc cgttccccga
    15901 ggaccaccgt cgtcaggctg tcgatccggc agtggttccc gctcgcgccg cacccaacgg
    15961 tcgcctggca gacgaatgtg tccttccgct cgacccgagc tgacagaaaa cggtcgaggt
    16021 cgagcccgtc ggctcgatcc agggagatcg tccctcgggc cagcagcgcg atgccgagcg
    16081 cgcccacggt gcccgggtta ggggggatga tgatctctcc gccggtttgg cgcgcgactg
    16141 cggcggccag ggcgtccgag gcgaaaggca tgccctggca gaagatcacg cgtccgaccg
    16201 aacggctgcc tttgacgcgg ttcaggtagt tctggatgat ggaatcatag aggccggcca
    16261 ggatggcgct ctgatcgacg ccggcggcca ccgcctcgtc gatgacctcc gccatgaaga
    16321 ccgaacagtg ttggcccaga gagacgccgt gggatgcatc gagggcttcc cgtccgagtt
    16381 gggccacgtc gtccaatccg gcgaacttgc gtccctgttc ctcgatgaac gagccggtgc
    16441 ccgcgctgca cgcctcgttc atggcgcaat cgatgacacg gccttcatcg agtcggatgt
    16501 acttggcgtc ctggccgccg atctcgaaaa tggtgtctac gcgcgcgtcg aagtggaggg
    16561 cgccttcggc gtgagccaca atctcgttga tgatcaggac cgcgtcggag ccgtagcatg
    16621 ttctgcagag cgaccccaca atctcccgcc cgctgccggt cacacccagc ccgaggacag
    16681 ggaacctccc ggccggtccg tcaacgaacg ctcgaatcag ggcctgggct gcgccgaccg
    16741 gatcgcctcc cgttcggcgg taacaatccc agagcgcttc cccagccacc tcgtccaacg
    16801 ccaccagctt cgacccggtc gacccgatgt ccaatcccag aatcagccgg cgctcgagcc
    16861 cttcgatccg gggcgccacg ggcgagacca tgcgccgcac caggggcagc gattgagcca
    16921 gcgccggcaa ccgttcgagg cggctccggg tctctccggc gatgacttcc gacagcgtcg
    16981 ggaggggcgc ctgccgctcg gccgcgacaa gggcgctccc gagggcctcg aagaagagcg
    17041 cgtcctcggg agccacggtt cgggattgaa atccgttctc ggagagaaag cgcgccacag
    17101 tccgctgcac acgcagcgaa cggctcaccc cgccgatcaa ggcgaccggg ctggggctga
    17161 gccgcggttt gaggagagtc aacacattcg cccccaccgc gtcgaacaat ccggccagaa
    17221 tgcggccccg gctttcgccc ttgttggcca ggtgcgtcat atccgtcttc aggatcaccg
    17281 ggcatcgccc cgaaagcggc gcggggtccg agacatcggc gcacagggca ctggcggccc
    17341 cgacatcgag cccgaagcgt tcggtcaatt ggcgcagaaa attgccggtc ccttgcgaac
    17401 agcggctgtt ttcccggaag atttcgatgc ccttgtctcg cagttcgagc accgagaagc
    17461 cgtggctgcc gatcgagatg accgtggccg gatcgttgcc gaacaggaac cgataagccc
    17521 tcgcctgggc ctgcttggtc ggcacacgcg gcagtcgcac cagccgggcc agccgtccgc
    17581 tagaagcggc cccgctcaac gtcggccaat cgaagtccgc cagcgccgca accagccgcg
    17641 gacccggctg cttcgcatgc tcaaaatgca cgcgacgggt ccatcgccat tgcccgtcaa
    17701 cccgcgtcag ttcgacaatt ttgatggtct cgccgcccag atcgatgccc aggacgcggg
    17761 tcggggcgcc cccgccctca ctcgcgggtt ccgagccgcg cagtgacgac gccgaacccg
    17821 cctgcggagg cgcaggcggc gctcccggcg gcggacgttg tccggggccg ggcggaggag
    17881 cgtgtgttgc actcatggga gccgcggatc agcgtgctaa ctgatgacag ccacgcggcc
    17941 ccgattcaac ccgaaaccga tcgatccctc cagccccggg cacctcgaga tccagtcaca
    18001 ttgaccccgc agtgcatctg cgggctgtcg gcggggatcg agtctgcgtc gcgtggggcc
    18061 tgcctgccgc gcctgcctgt gccgtgcccg acatggcaga caggccgctg cgctttgcgg
    18121 cgcaggcagg gcacgcggcc tacagcggtt cgaagggata gactcttatc cggcgctggt
    18181 cgcagcgtcc atggtcggtg tccggctctt cgacatgggt aaaatgcgga gccgatgtca
    18241 tcgtaaagtg cactcatgcg cgctagcgga tttggacgcg gtagaaccgc tgcgcggcgg
    18301 ccggcgtttg gtcgacgaac gacgctgtgt tgccggcggc cgtgatgggg ctgccaaggt
    18361 cttgccaggg gccattcacg gcgctggcgg tctggacctg gtaggaggcg cccaccttcg
    18421 attgccactc gagggcgatg ccgcctgcgg cggaggtcac gctcagaatc ttgaaagact
    18481 cggccggcgg gacgtaggtg ggatcgatta ggaacgcctt gagtccgttg ttcacgccca
    18541 gagccaccag gtagcccgcg ccaaaatcca cattgccctg aaactcgatg tccggattgt
    18601 acgtggtgaa gagttcgtta tcgacccaga gcgggccggc ggccaggtcg cggacgtcgt
    18661 agacggggac gctgacgggg ctgggagggc tgccgttgcg caggccggcc aggagaccgg
    18721 tggcggcgtc gtacttgatg cctgtgaccg acgcagggac gctgcggtcg ccttcggtgg
    18781 ggtaggtcgc cttgacggcg cccagtccgg tgccgaggtc aaactcgatg aggtagaggc
    18841 tgccgacagc tttgacccag aaggtgttgt tgccggggcc ccaggccagg ccgaatcgga
    18901 agatgttggg cggcgcgtcg ggcacggtga tggtggtgga gatgaactcg tagttgggat
    18961 cgggtttgag aatggcgacg gtgcgcgctt ggagcggggt gggactccat ttgccggcgc
    19021 cgaccaggat ttcggtctcg aggccgccgc cgcgcacatc gatgttggcg ccccagccgt
    19081 cggtgggggc ggtgatgccg ttgccggggt cgccgctgaa ggcgagccat tggtcgggcg
    19141 tgtaggggct gtcgtcgccg atgccgtaga tggtgaacgg tgttgtgtcg gtgttgccgg
    19201 tgacgttgca gatgtagatc acgccgtcat cggcgacgtc gactttgttc attccgccgt
    19261 agagggtgag tccggagtaa tcgatgtagt gcttgtgttc gcccgtttgc atgtcgatca
    19321 ccgcgatcat gttggtcggg ttgaggcggg tgaccacgat gacgttggtg gtgagcgggt
    19381 tgatggccaa accgtactct ttgtatccgg atgtcagata gggccgtgtt ccgggcagga
    19441 cgttccagat gttctcgacc tgcggcgagg cgttcgaggg caggaccgtg agcgtcgctt
    19501 cggcgctggt ggccgagccg cttaggttgc tgacgacgac actgtaagcg ccctggctgg
    19561 cggacgcgat gttcgagagc gtcagcgtcg gggaggtcgc ccctgcgatc tcctcgccgt
    19621 tgaaccgcca ttggtaggtg aacggttgag tgccgctcac gccaaccgag aacgtgtagg
    19681 tctgggcgcc ttgccaaacg gtggcgttgg ccggttgggt ggcgatgccc gggggcagga
    19741 agacctcgat caggtcaaag gccatcatgc cgttgttgga ttccagggcg aagagtcttc
    19801 ccgcgtgaag ggcgacggcg ccggtgagat tgccattgga gtacccgccc gggaaatcct
    19861 tggcggcgtc ctggagttgg ggggcggcgg gattcgagat atcgtagagt cggagtctgt
    19921 gattggcggt gtcggccgag cctgcctcga caatgccgag cagggcgcga gcgggatcga
    19981 gatcaagcgg ggcgccgacg ataccggtga ccgtggttgt cacggccgcg gtgccggccg
    20041 tgagatcgag cgtgaactgc ttgagattgc cgccggcctg tttggcccag aacgtgttgc
    20101 caaggcccca ggccaatccc caccgcgtgt cgtcggccgc gcaatcggtg acaatctttt
    20161 gcgcggtgaa agtcattcca tccgcggtgg tgagcagggc gacgttcttg tcgcgcgtgc
    20221 cgagcagaat ctgcgtgttc gagcccgtgc cacgcagaac aagcgaatcg ccaaaccggc
    20281 ggttgttggc gactccgtcg gcttccgaag gatcgccgga atagaccagc tcgggctgcg
    20341 ccgcctcgtc cgcccagcga tagagacgga acgggccgct ggcgccggtg gtgtcgacgg
    20401 tcaggttgcc cacatagatc acgccatcgt cggtgatcgc aaccatgttt accgggaacg
    20461 tgccgccggt gatcacagaa gtgtcaaaag gaagcgtccc gagttccccg cccgtgccgg
    20521 cgtccaggac atgcaccgcc gccgagccgg tgcggctcgg cacgagcaag tgatcggtcg
    20581 tggggttata agccagacca cgcgtcaagt gggtggtgtc gagaaacgcg acgtcacccg
    20641 gcgcgaggct ccaaaccggt gtgagttggt aggattgtgc gcgcgcgctg aagccgagcg
    20701 tcagccccag catgaggagc agcaggaatc ggacaacagg gcagggattg ggctcgttcg
    20761 agtgtttcat ctcaggatgt gtcgttcgtt gttgttcatt tcgtcgtggt ctcggacgat
    20821 ggcgcggacc gctgggccgg catccgcgtt tgcatggatg ctgacgcgcc ggaactgcac
    20881 gcgtcaagcg catccggctt cgaatcggcc cagccccgaa accgtgccat gactttaggc
    20941 ttgaacgctc gcgcgcatga aggaatgtag aatccttgac tggtcagact ctttgtggac
    21001 aagtcaagtt tctgagcctc gttgacgggg tcaaggattc tagagaagcg aggcgcggaa
    21061 gagaacggcg ggaaaacggc ggggctggtt catgaggttg aggattgaat tggtctgagg
    21121 cgcgcctcgc ggggaaccga gaatgaaaat gaatgcgtat gaaaaagcgg ctgtcgcgga
    21181 tggcgcgcat gcaaggccat gcgggcgaca gtgcggattt actctgatcg agttgcttgt
    21241 ggtgatcgcg atcatcgcga tcctggcggg gatgctgttg ccggcgctgt ccaaggcgaa
    21301 ggtaaaggcc caggccattg cgtgcctgaa caatgtgaaa caacttcagc tcgcctacga
    21361 gctttacgcc accgacaacg gcgacacgct gatggacaac tcggtcaccg gcgtcgccag
    21421 tcctggcgac aaggcctgga tcaaaggcaa cgttcaggag tatgccgccg actaccttga
    21481 tcatccccga ttcggcgttc ttttcctcta caacagttcc gtcgagatct atcggtgtcc
    21541 ttcgagccgg gcttatattc agggtgtggg ccgcggctcc ggcgcacggg tgacgcacaa
    21601 ccgttcgtat gcggtctcgg tctggctggg caacaacctc gacctgggcg cggatcgcac
    21661 cggcctgatt gcgcaccgat tcagcgcggt cagaaatcca tcgtcgacgt ccgtcttcat
    21721 cgaggagaat gcgatcagca tcgacaatgg tgcgatcggc ttcaaccggc ccattccggg
    21781 caatgcaggc agcgatcccg gcggggtctg gaacctgccc gcgaatcgcc acaacaacgg
    21841 ctgcagcttc tcctttctcg acggccacgg cgagaccttt cgctggcgcg gcccccggat
    21901 caaggagttg aacatgcagt attccgccga ccacagccgc tcgcagcgcc cgtcggcagg
    21961 ggtcaacccg atccacagtc tgccgtggga gtccaaggac ccggattacc agcgcttggc
    22021 tgacacggcg ccgctgcttt gaggctttag gggccgtcag gaaataataa gtgcaccggc
    22081 aaaggcggcg tgggcggcgt gggcggcggc ttgcaactgc gcttcggctt ggtccaacac
    22141 gccctcggtc agaccacgaa tttccagatg cacctccact ttgccgggaa tcacattggg
    22201 cgcgcccggc aagacggtga gtttgccgat ggt
//
LOCUS       DAOQRO010000006        21409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1669_length_21409_cov_5.710921, whole genome shotgun sequence.
ACCESSION   DAOQRO010000006 DAOQRO010000000
VERSION     DAOQRO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 21409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21409
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_21409_cov_5.710921"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1875)
                     /locus_tag="PKK20_00500"
     CDS             complement(199..1875)
                     /locus_tag="PKK20_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98402.1"
                     /translation="MNRRTFVKRTAGVTAGLLTSDRLFAASPVSPTGAAMPTWSFRPP
                     LSLEASLRLAGQNLLAILNPEDNYLPYWELSVSPRYEAALKRWWPAHNIGRWLDAMLR
                     LRQVMGFDIPEPIEKAMVENTKRFFDNPDHISLNPDRWPLHPHTTEEGLQWDLHSLRE
                     GLLALNALARWHQSDWAADTGRKMIQSVDAKLRDDGRWELEQFDACRKRGKQVIHNTA
                     PCDTHGRMIEAVIWFFETTGDPVALRFAHRLAEYHFANTTQPDGEIPASAQVDHTHSY
                     LGTLRGLLLYGRLTRQQKYLERVAAAYRVNIPRLVKESGYTSHNILIESFGETTSPGD
                     AAQLALWLARAGNAEFLDDVDRLVRARILPSQIRETPSLHPISRFGKDATRDLEKRII
                     GGYGGCHNHPHGHKVAVTDVTAADVHTLLDIYEHMVLLDDQTLEVFCHLDYDGPHATV
                     LSRRGQRAELTVIPKIERAVAIRAPRWTPVESIRVRVGGDLVHPVYAGHFARLGRQPA
                     GTRIVMEYDLPERRTRETDLGSPYEIAWRGDEVTGVRPNTTLYPFYPELR"
     gene            complement(2146..5325)
                     /locus_tag="PKK20_00505"
     CDS             complement(2146..5325)
                     /locus_tag="PKK20_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNU98403.1"
                     /translation="MSTPPRTSRWRFLRRALRWCRFGLSFLVLALLLALLYVTRVGLP
                     GFCKVPLLSELRRRGIDFEYERLAWHWHRGLVAQNAALRPLPDAGGFRARAAEIVLDP
                     HWGRLMRARQIELESLRILDGHLSVPLHEPGQTPDEFSIEAVNAGIRFLPGGTCEIEN
                     LEASFLGATVQASGVITNAHDLIEWLHTLDRRERPDPTPPWRAPLRRLAEFRKGLRLA
                     GVPEFRLRFRGDARHPESFAGEFKGKTDGAESPQFSVRNLRCSIDLSTNQPRPLAGAW
                     RLIARVEADSAGGSWGSLRHAEFRAHTDLQTTHPIPARVDWEVVARKAQTPWASFDGV
                     RLAGHSERLGANAREWRTSLTLDAQACTAPWGGTRTSRGAASFEHSLAGLLSAPIDLK
                     ASIEAVDTPPVTIGVAHLEARLRSVPDAVGGGGPLATLPGSQRGWEILNRIEGSWRIA
                     ATNLQSQCLAQPLTLEDVIGAGDWKAPCLRVASLRTGLYGGSLDLSAARLDVATGEVE
                     AQLLSDFDPHAVAALAGQRPSKWLDQFQWDRPPRVQARVRALLPASGDRDPDWARRAL
                     LHVRAEGRIEGENTSYRGVLIDRGALSLSLSNQMLRLRDFHVVRPEGDADLAYDLHTL
                     TQEFRWRLRANLDPKGTCAAINLDAPRLLSAFDFTGPAAVSGEAWGRWGPDKEVSFAL
                     SGIVTNFTFRSESIDELRADVRMAHQFLSASNVALRTGAEWIRAPGVGVDFVESRAYV
                     TNAQAELDPMRFARVVGSNLVRTLRPYRFDRPPRIHADGQVPIRGAMDDADMRFSVAG
                     GPFHFWRFNIPDVDGRVHWEGERVTLTNVVCDFYEGRLEGQLQADLRPDGSADLAFRA
                     AVTNLNLQPLVLDTIDTINRIEGIAQGEFELTHANSADWGSWNGSGRLSMRDGLLWDL
                     PILSVLSPVLNAMVPGLGNNRARAATGSFVITNSVIYTDDLEIVAQPVRLAYRGTLDF
                     NWNVRARVEAEVIPGVPLIGPLLNIIFAPVSKALIFRISGTLGEPELEPLLVPRFLQP
                     ILRPVHTIRGVFSSPHTPQTTNAPPKGVP"
     gene            complement(5327..7159)
                     /locus_tag="PKK20_00510"
     CDS             complement(5327..7159)
                     /locus_tag="PKK20_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNU98404.1"
                     /translation="MNAKTTRWLLFLAVALAAFIALFERRTDDTARRAEQAARLLPEL
                     VTAEVTALTLTGATNPAIRLERVHDGWEFQSPLRYPAQPAGVDRLLTALRDLRWQGHV
                     TAQELLAHTNGLAAFGFAPPAVSIAIQQGDRPIDLRLGAPTALGGQIYAQVAGRGGLY
                     TVDGAVLKTLPGGVNEWRDTALVRLARRRFDRLQVRPITNGFEVVRSPSNHVWRMVRP
                     LSTRANNARLDLLLDQLELARVIQFVTDDPRADLEPYGLQPPDRELVLSQGTNDILTL
                     QIGRSPANAPDQLFVRRMEHSNVVAVARSSLAPWLASFREFCDRRMMVFDPDAVTRIE
                     GIADGAFALERQTNGTWQIVAPFLASADSVLVLEMLQNLAELEFLEFEREVTTDFVPY
                     GLAPPRRQYVLQTTVAGAGSAPTNQVLAQVGFGNPTGFKYYARRSLDSAVVTIIDNNR
                     LPRAAFELRDRRIWNVSTNQMVSITVHQLGETRKLLRTGPMQWTAPPGDTAQLNPVSL
                     EEAAYRLGQLRADRWVARGEDQLARFGFGTIDHRLAIEVNQDGRIRTLEVRFGRRTLS
                     NQSAYAAVSIEGVEGPVIFECPRSIYEFVLGNLTLVPTLPDGSG"
     gene            complement(7165..8664)
                     /locus_tag="PKK20_00515"
     CDS             complement(7165..8664)
                     /locus_tag="PKK20_00515"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNU98405.1"
                     /translation="MPHPSDPTESFSSASRFRIALNVALALLALAALLAMANYLAARH
                     FQRFLWAEDERYQLSLPTRQVLAAIANEVKVTILFSRNNTLYSPVTGLLKEYGYACPR
                     LRIETIDYDRDVQLAQTTAARLQLSPEDSDLVVFEGEGRTRIVRAVELSEYDISGLLA
                     GGKEVRRVGFKGESLFTDALANLIEGRSPKVYFLQGHGEHDPTSLETRFGYSQFTRLL
                     QQKNIATATLRLVGDAEVPEDCELLVLAGPRSRIDPTALEKIGRYLDTGGRMLALLSF
                     YQSGYAQTGLERFLAGWGVAVGDNLALDPSNAVRGGGILATNFSNHPIVRPMRGHTVY
                     LLVPRSVAPLRLGGAAGDAPKVEPLVSTSVGGRTESEGLSSGLPEFNPARDRRGVIPL
                     AVAVEKGSIQGVAADRNSTRLVVVGESIFLANETIVKSAANIEFASLAVNWLLDRPQH
                     LSGLAPRPIKEHSVLLSQSQVVQLRWILLAVLPGTPLLIGLLVWWRRRR"
     gene            complement(8666..9421)
                     /locus_tag="PKK20_00520"
     CDS             complement(8666..9421)
                     /locus_tag="PKK20_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU98406.1"
                     /translation="MRVLITLIRRELSSFFSGLGGYLIIAAMLFLTGVSFVDIIEAVN
                     NDTIHTPLTELFFGSPFFWLILLVVPTPVITMRLFAIEKFSGTYETLMTTPVGDVQVV
                     LAKFAAAWMFFAVAIAPLLGVLQLVHHYASPGERFEWWTTASTFGGILLIGSSYLALG
                     CLASALTRSQIVAATMSFGAGLALFLFSFLQFSIGTQPGCLATVVNHVSLFGHMEDFV
                     RGVVDTRPLVFHLSLVAFVLFLNVKVVESRRWK"
     gene            complement(9421..10383)
                     /locus_tag="PKK20_00525"
     CDS             complement(9421..10383)
                     /locus_tag="PKK20_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98407.1"
                     /translation="MIEIEDLTKRYGRHAAIDRISFSVCRGEIVGLLGPNGAGKSTIM
                     RILACFMPATSGTARIAGLDVFREADSVRRRIGYMPENNPLHLDMRVREYLKFRARLK
                     GLGIAESRDRVSVVMDQCGLSEVGWKIIGTLSKGFRQRVGLADALVHEPDLIILDEPT
                     IGLDPHQVRAVRQLIKELGRHRTVLLSSHILPEVEMTCHRVLILHGGRVLAADTTENL
                     QRRMSDSGQVVAEIAANPHELAACWDGMEGVERFDVTPSDGLYCRCALTPQHGVDLRV
                     RIFETVLARGWRLRELTHSRHSLEDIFVHVTRADREEEEEEEGL"
     gene            complement(10452..11855)
                     /locus_tag="PKK20_00530"
     CDS             complement(10452..11855)
                     /locus_tag="PKK20_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNU98408.1"
                     /translation="MSSVRSAVLACAGGAGVVLAIWAGALGLWLPDSFGAEFDVRLDS
                     AVEAVAQVMPAVVNIGTETIVAVRDPFEDLLQEFWAPYHRRRPFTTTQYSLGSGVIID
                     EAGYLITNDHVVRRATRIRVRIGDEAGSREYEAKLVGESSRNDIAMLKIEARPGERFR
                     TVRFAADDDLLLGETVLALGNPFGLGGSVSRGILSSKSRRPALENTPMGIEDWLQTDA
                     AINPGNSGGPLINLRGELIGLSIAVYREGQGIGFAIPIKRITEALLEIASPELRQYWF
                     GARVRPGGSTLAVSSLEEGSPAEAAGLKPDDMIVGINGRATRGFLDFIKELSAAGDER
                     DIALTLRRGSDRRSVTLRMVRAGAVFNAALIRRKLGLAVQEISPELSGRLGLADNAGL
                     VIADVDRTGPASEARLSRGAIIVAVDGRAPEGVVESAKYLYGKKKGDTVGLEVLVQRR
                     RGMVLTWESLEIALQVQ"
     gene            11954..13474
                     /locus_tag="PKK20_00535"
     CDS             11954..13474
                     /locus_tag="PKK20_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU98409.1"
                     /translation="MLTPSQKTAREIECLIRARYPILYLVSSEEARVESVICGIARRW
                     EKQVYQWSCTSGLVPAGVSVQSQKQRHSATKDPLAALDQVVDQVEPALFIFKDLHPFL
                     VRSNYAVIRRLKEIALHLKNSLKTIILISPVLEVPIEIDKEITVLPFPLPGCDEFGEL
                     LDQVIAEVAPTGRVALDLDGAGRERLLRATLGLTLSEGENVFAKILIKHERLSGDLVA
                     EVLEEKQQIIRKSGLLEYYATEETFDTVGGLAQVKDWLAKRASALSDAARDFGLPAPK
                     GMLLLGVQGCGKSLCAKAVSNAWQVPLLRFDIGRVFGSLVGSSEENVRRAIAVAESVA
                     PAVLWVDEIDKAFAGSQWSALADGGTTARVFGTFLTWLSEKTVPVFVLATANDISQLP
                     PELLRKGRLDEIFFVDLPDAAERLEILSIHLRKRNRDPAGFDLDALTRASAGYSGAEI
                     EEAVVSALFDAFHARQQLETGHILTALEQSVPLARTMSEQMDRLRQWAVGRARNAR"
     gene            13930..14946
                     /locus_tag="PKK20_00540"
     CDS             13930..14946
                     /locus_tag="PKK20_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNU98410.1"
                     /translation="MKAVLQTSVLVAALSALSLLGAADQPRPLRLGHFPNLTHAQAVY
                     ARAGGQFDRKIGRAVEWTSFNAGPTAIEALFTDAIDVAFVGPNPTINGFLRTRGEKFV
                     VIAGGASGGTGLVVRSGAGIRSEADFDGKIIATPQLGNTQDVAARLWFAEHGYRLKEK
                     GGSLTLVALSNPDQLTLFRRGEIHGAWTVEPWVARLELEGGGELFLDERTLWPEGRYV
                     TTHLVANRVFLREHPQLIRDLLAALIEVTQEINGDKNAAARILNAELKRETGKALPEA
                     VVKRALQRIEFTWDPISASLRKCGEAAYRVGFLRSQPRLAGLYSLDALNDVLHAKGLP
                     PVAD"
     gene            14949..15794
                     /locus_tag="PKK20_00545"
     CDS             14949..15794
                     /locus_tag="PKK20_00545"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98411.1"
                     /translation="MQPPAAPDHPPSAEPAPAPPGVKLRLANVSKVFRNNEQTIEALA
                     PVSLEIREGEFVVLFGPSGCGKSTLLNLIAGFDAPTTGEITLEGRRVRRPGNDRLMMF
                     QEHALFPWLNVLDNVTYGLRWLPNFRFHPLRRRRRAMELLKLVRLDEFAHASIHELSG
                     GMKQRAALARALAPDPKLLLIDEPFPALDALVRAKLYGELQDILVRTRKTIVSVTHDS
                     REAACLGDRVVVFTKRPGRIKAEIKVDMPRPRDINAAAVTAYAAQIAAQLEEEPREND
                     DGKRD"
     gene            15766..16545
                     /locus_tag="PKK20_00550"
     CDS             15766..16545
                     /locus_tag="PKK20_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU98412.1"
                     /translation="MRTTTEKGTKRYVAGFYLLLLGGWQTLFSLQLIPDYLFPSPLQV
                     GERLWELGRDGLLWPSVSATLMRMTIGFSAAAGLGLVVGVVMGMNHVLNQCLRSLFLG
                     LQTLPTAAWVPISLLLFGLSDRGIYFVIVMSSVAAVAIATSDGILHIPPLHLRAARTL
                     GTPGYAMPLRVILPAALPGIVTGVKLGWTLGWHGAVSAELIKSSVGLGYLLYMGRELN
                     DVAQVLGIMMLTILFGLLLDRFLFGQIERRIRTRWGLTSQS"
     gene            16642..18027
                     /locus_tag="PKK20_00555"
     CDS             16642..18027
                     /locus_tag="PKK20_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNU98413.1"
                     /translation="MAANIQTPATTPPGAPSGHLTTYLLGSAVVAALGGLLFGFETAV
                     ISGTTEWLQKHFELTDFMLGFTVSSALIGTIVGAISVGRPADAWGRRGVLFILGILFL
                     LAALGCAFAWNWHAFMFFRFLGGLGVGGASVVSPMYIAEISPASYRGRLVAVTQFNIV
                     LGILLAYLSNYIIGSLGLGDAQSRWMFGVMALPAIVFLLLLVFTPQSPRWLVAQGRIA
                     EARRVLEKCGTDLNALDFELGEIQASLDLAHHGLEEPFFCRRYLKPILLAVAIAMFNQ
                     LSGINALIYYTAHIFKMAGADQASALLQSVIIGFTNLVFTLAALTVIDHFGRRRLMLL
                     GSIGYIASLSASAYAFYTHSGGWLLLGSLLVFIASHAFGQGAVIWVFISEIFPNRVRA
                     RGQALGSFTHWIMAAAISWTFPMIAAKSGGHVFAFYALCMVGQLIWVIRVMPETKGIS
                     LEQIQKRLGIE"
     gene            18073..19353
                     /locus_tag="PKK20_00560"
     CDS             18073..19353
                     /locus_tag="PKK20_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNU98414.1"
                     /translation="MKTQTRIPALVALLLSSLPCAAADLWNVRDFGAKGDGSTDDTAA
                     FQQALDAAAKAGGGTVSAPRGNYLFAGHLNVPTAVTLRGVWESVPAHNGLRDRGLPKP
                     TDDGTTFLVTENAGKEGGLPFITLNHNSTLKGVALYYPNQKADDIPVPYPWAVALRGK
                     NPAVLAVELLNPYNGIDATQNERHLVRDVHGQPLRRGILVDRIFDIGRIENVHFNPWW
                     STRPKLFQWQMENGEAFVFGRSDWQYVLNTFCFGYKIGYRFIKSESGVCNGNFLGLGA
                     DDCHTSLVVDESAPFGLLITNGEFVSFHGPDPTMVEVMGTHTGAVRFVNCAFWGPCHQ
                     IAKIDGRGTVGFSDCTFVQWDSKREGRHAIRANGGTLLVRGCEFREAKPQIHLAEGVR
                     RAVITDNVFNGAARIANLSTGSVQIANNAGDKGH"
     gene            19382..20224
                     /locus_tag="PKK20_00565"
     CDS             19382..20224
                     /locus_tag="PKK20_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU98415.1"
                     /translation="MNLPSVLLLIPAYNEEGRIGPTLRQYASYVRDHYAGRFEIVAVV
                     NGCRDNTLGVVESVARDFSNIRWLVIPDRVGKGGALIEGLRLAPEADIIGYADADAAT
                     SPASLLELAARCRDYDCAVGSRRVPGAVIHQLQPSHRQLASKIFHFIVEFLFGMGIHD
                     TQCGAKFLRQHMALKIQDRLLIADMAFDVNLLYAVKHAGGTMIEVPVDWTDHLGSTVR
                     YFRTSLVMFLSVVRLRLVYSPFYSWLGPIRPLEAWIYKALRNPPPLAVPPPDRPATPT
                     PPER"
     gene            complement(20294..20560)
                     /locus_tag="PKK20_00570"
     CDS             complement(20294..20560)
                     /locus_tag="PKK20_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNU98416.1"
                     /translation="MNCDELLSLLNEYVDGETAAGLCVEFERHLAGCNPCQVVVDNIR
                     KTITLYKAGEPYELPLAFRNQLHAALRARWQQTHTPAAPPPPTP"
     gene            complement(20598..21287)
                     /locus_tag="PKK20_00575"
     CDS             complement(20598..21287)
                     /locus_tag="PKK20_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU98417.1"
                     /translation="MTPEEPRPKSSPSEAGDSPADIELVRRAQAGELAAFELLVNRHE
                     RHIYSLALRILRQEQDAEDVTQTAFLSALENLAGFRGEASFATWLARIATHAALKVIR
                     KREGLDLVSFDHAVDPAAGGEAIPHPEYIADWRQSPERLVERNETRELIEAALAELDE
                     KHRLVFLLRDVEGFSVRETAEFIGISEVNVKVRLLRARLQLRERLTKVFGDPARRLAP
                     HRHDEDAAPAV"
BASE COUNT         3773 a         7059 c         6792 g         3785 t
ORIGIN      
        1 gactacgcgc caaaccctct acccagcaga caccaatggc cgactgtacc atgctgaccc
       61 atttccagcc gcgaaagaac acaaggacca caaagaaggg acgtgtcggc tttgcgctct
      121 ttgcgttctt ttgcggctga agacctccgc cgccgcaacg ttgcccagac cgcacccgcc
      181 ccgggtcggg cctccaggtc aacgcagctc cgggtagaag gggtatagcg ttgtattggg
      241 gcgcactccc gtgacttcat caccgcgcca ggcaatttcg tacggactcc caagatcggt
      301 ttcccgggtg cgacgttcgg gcaggtcata ttccatgacg atccgggttc cggccggctg
      361 gcggccgagt cgggcgaaat gacccgcata aaccgggtgc accaagtcgc cgccaacacg
      421 gacacgaatg gattcgaccg gagtccatcg cggtgcccgg atcgccacag cgcgttcgat
      481 tttcggaatg accgtcagtt ccgcgcgttg gccccggcgg ctcaataccg tggcgtgcgg
      541 tccgtcgtaa tcgagatggc agaacacctc gagggtctgg tcgtccaaca gaaccatgtg
      601 ttcgtaaatg tccagcagcg tgtgcacgtc ggcggcggtc acgtccgtga cggcaacctt
      661 gtgtccgtgg ggatgattgt ggcagccgcc atagccgccg atgatgcgtt tctcaaggtc
      721 gcgggtggcg tcttttccaa agcgggagat cgggtgcaag gaaggcgttt cacgaatctg
      781 cgagggcagg atgcgggccc ggacaaggcg atcgacgtcg tcaaggaatt ccgcgtttcc
      841 ggcccgggcc agccagagcg ccagttgggc cgcatccccg ggcgacgtgg tttcgccgaa
      901 gctttcgatc aagatattgt ggctggtgta tcccgattcc ttgaccaggc gcggaatgtt
      961 cacccggtag gcagccgcca cgcgctcgag gtacttctgc tgccgcgtca ggcgtccgta
     1021 caggagaagt ccgcgcaatg tgcccaggta ggaatgggtg tgatcgacct gggcgctcgc
     1081 gggaatttcg ccgtcgggct gtgtggtgtt cgcaaaatga tactccgcca gtcgatgggc
     1141 aaaccgcagc gccaccggat cgccggtggt ttcaaagaac cagattacgg cctcaatcat
     1201 ccggccgtgg gtatcacacg gtgccgtatt gtgaatgacc tgcttgccgc gcttccggca
     1261 ggcatcgaac tgctccagct cccaacggcc gtcatcgcgt agtttggcat cgacgctttg
     1321 gatcattttc cggcctgtgt cggcggccca atcgctctgg tgccaccggg ccagggcgtt
     1381 gagagccagg aggccttcgc ggagcgaatg caggtcccac tgcaagccct cctcggttgt
     1441 gtgcggatgc agcggccagc gatccgggtt caggctgatg tggtccgggt tgtcgaaaaa
     1501 gcgcttggtg ttctccacca tcgccttttc gatcggctcg ggaatgtcga atcccatgac
     1561 ctgccgcagc cgcaacatgg catccagcca gcggccgata ttgtgggcgg gccaccagcg
     1621 tttgagggcc gcttcgtatc tcggactgac ggacagctcc caatacggca ggtaattgtc
     1681 ctcgggattg agaatggcca gcaggttttg tcccgcgagg cggagcgacg cctcgagaga
     1741 cagaggcggc cgaaatgacc aggtcggcat ggccgcgccc gtcggcgata ccggactcgc
     1801 ggcaaacaac cggtcggagg tcagcaaccc ggcagtcacc ccggcggttc gtttgacaaa
     1861 agtgcggcgg ttcattcgat gagccattcc ggtcatgttt cctgtggcgg ttgtcgattt
     1921 cattgctgct cctgtgcttt gcctatcctg cggcctgccg cgccggtcgg atgccacggc
     1981 cagtttcggc cggtggattt gtcgttttcg ccgtgtccaa ctcgctgagg catgttcaaa
     2041 ttcgcgaata aggcggcgta aataaatgtc ggttttcgcc ccgccctcct acgcaccgct
     2101 gctgagctgc ataggatgca cccgccttga cgcttacaat gcaggctagg gcacgccctt
     2161 tggaggcgcg ttcgtggtct ggggggtgtg cggggaggaa aacacgccgc ggatggtgtg
     2221 cacgggccgg aggattggct gcaggaatcg cgggaccagg agcggttcga gttccggttc
     2281 gccgagggtg ccgctgattc ggaagatgag ggctttggag acgggggcga agatgatgtt
     2341 gaggagcggg ccgatcaggg gcacgcccgg aatgacctcc gcctcgacgc gggcgcggac
     2401 gttccagttg aagtccagcg tgcctcggta ggcgagccgt acgggctggg ctacgatttc
     2461 gaggtcgtct gtgtagatga cgctgttggt gataacgaag gagccggtcg cggctcgcgc
     2521 ccgattgttt cccagcccgg gaaccatggc gttgagcacc ggcgacaaca cgctgaggat
     2581 aggcagatcc cagagcaggc cgtcgcgcat cgaaaggcgc ccggagccgt tccaactccc
     2641 ccagtccgca ctgttggcgt gcgttagctc gaattcgccc tgagcaatgc cctcgatgcg
     2701 attaatggta tcgatggtgt ccagcaccag cggctggagg ttcagattgg tgactgcggc
     2761 gcggaacgcg agatcggcgc ttccgtcggg ccggagatcc gcctggagct ggccctctag
     2821 gcggccttcg taaaagtcgc acacgacatt ggtgagggtc acgcgctcgc cttcccagtg
     2881 cacccggccg tcaacgtccg gaatgttgaa tcgccagaag tggaacggcc cgcctgcgac
     2941 ggagaaccgc atgtcggcgt cgtccattgc gccccggatg ggcacttgcc cgtcagcgtg
     3001 aatccgcggc gggcggtcga agcgataggg gcgcagggtt ctgaccaggt tcgagccgac
     3061 gacacgggcg aatcgcatcg gatcgagctc ggcctgggcg ttggtcacat aggcgcggga
     3121 ttcgacgaaa tcaacgccca cgcccggcgc gcgaatccac tccgcgcccg tgcggagcgc
     3181 gacattgctg gccgacagga actggtgggc cattcgaaca tccgcgcgca gttcatcgat
     3241 cgactcgctg cggaaagtga aattggtgac gatcccgctt agggcgaagc tgacttcctt
     3301 gtcgggaccc cagcggcccc aggcttcgcc ggacacggct gccggccctg tgaaatcgaa
     3361 cgccgagagc aggcgcgggg cgtcgagatt gatggcggcg caggtgcctt tgggatcgag
     3421 gttcgcgcgc aggcgccagc gaaactcctg ggtgagcgtg tgcaggtcgt aggcaagatc
     3481 tgcgtcgccc tcgggccgca ccacatgaaa atcgcgcagg cgcagcatct gattggacag
     3541 actcagggaa agcgctcccc gatcgatgag cacaccccgg tacgacgtgt tctcgccctc
     3601 gatgcgcccc tcggccctca cgtggagaag agcccggcga gcccagtccg gatcgcgatc
     3661 gcccgacgcg ggcagcaggg cgcgcacacg cgcctggacg cgcggggggc ggtcccactg
     3721 gaactggtca agccacttgg acgggcgctg acccgccagc gccgccacgg catgcggatc
     3781 gaagtccgag aggagctgcg cctcgacttc cccagtcgcc acgtccaggc gcgccgcgga
     3841 cagatcgagt gatccaccat agagcccagt ccgcaagctc gcgacgcgca gacacggcgc
     3901 tttccaatcg cccgcgccga tcacgtcttc gagagtcagt ggctgagcca ggcactgaga
     3961 ctgcaagttc gtcgccgcga tccgccacga gccctcgatc cggttcagta tctcccatcc
     4021 acgctgtgac cctggcagcg tggcaagcgg gccgccgccc ccgaccgcat ctggaacaga
     4081 ccgcagacgc gcctcgagat gcgccactcc gatcgtaacc ggcggggtat cgacggcctc
     4141 gatgcttgcc ttgaggtcga tcggggccga cagcaggcca gccagcgagt gttcgaatga
     4201 ggcggcgcct cggctcgtgc gggtgccccc ccacggggcc gtgcacgctt gggcatccag
     4261 tgtcaggctc gtccgccact cccgggcgtt cgcgccaagg cgctcggaat ggcccgccag
     4321 gcgaacgcca tcgaaggagg cccagggcgt ctgggccttg cgcgcgacaa cctcccaatc
     4381 gacccgggcg gggatggggt gggtggtctg aagatcggtg tgcgcgcgga actccgcgtg
     4441 tcgcagactg ccccaagacc caccggcgga gtcggcttcg acgcgcgcga tcagccgcca
     4501 ggcgccggca agcggccggg gctggttggt gctcagatcg atgctgcagc gcagattccg
     4561 aacgctgaat tgcggcgact cggcgccgtc ggttttccct ttgaactcgc cggcgaacga
     4621 ttccgggtgc cgggcgtcgc cgcgaaaccg cagcctgaac tccggcaccc cggccaggcg
     4681 caggcccttc cggaattcgg ccaagcgccg cagcggcgcg cgccagggcg gggtcgggtc
     4741 cggtcgctcc cgtctgtcga gcgtgtggag ccactcgatg aggtcgtgtg cgttagtgat
     4801 gacgccgctg gcctgaacgg ttgcccccag gaatgaggcc tcgagattct cgatttcaca
     4861 ggtgccgccc ggcaggaacc ggatgccggc gttcacggcc tcgatcgaga attcgtcggg
     4921 tgtctggcca ggttcatgca agggcaccga gagatgaccg tcgaggatgc gcagagattc
     4981 caactcgatt tggcgcgcgc gcatcaggcg gccccaatgc gggtcgagaa cgatctcggc
     5041 ggcccgggcg cggaatccgc cggcatcggg gagcggacgc agggcggcgt tctgggccac
     5101 caggccgcga tgccagtgcc aggcgagccg ctcgtactcg aagtcgatcc cgcgacggcg
     5161 caattcggag agcagcggca ctttgcagaa gccgggcagg ccgacgcgcg tcacatacag
     5221 cagggccaag agcagggcga ggacgaggaa agagagcccg aagcgacacc accggagggc
     5281 gcggcgaagg aaacgccatc tgctcgtccg cggaggtgtg gacatatcag ccggacccgt
     5341 ccgggagtgt cggcaccaag gtcaggttgc cgaggacaaa ctcgtaaatc gagcgggggc
     5401 actcgaagat cacagggcct tccacgccct cgatggagac ggcggcatag gcggattggt
     5461 tcgacagggt acgccgtccg aagcgcactt cgagggtgcg gatgcgtcca tcctggttga
     5521 cctcgatggc caggcgatga tcgatggtgc caaagccgaa gcgggcgagt tgatcttcgc
     5581 cgcgggccac ccagcggtcg gcccgcaatt ggccgagtcg atacgccgcc tcttcgaggc
     5641 tgaccggatt gagctgggcg gtgtcgcccg gcggggccgt ccactgcatc ggtccggtgc
     5701 gcaggagttt tcgggtctcg cccaactggt gcacggtgat gctgaccatc tggttggtgc
     5761 tgacattcca gattcggcgg tcgcgcagct cgaatgcggc gcgcggcagg cggttgttgt
     5821 cgatgatcgt gacgacggcg ctgtccagcg aacgccgcgc gtagtacttg aatccggtcg
     5881 ggttgccaaa gccaacctga gccagcacct gattggtggg cgcggacccg gcgcccgcca
     5941 cggtcgtttg gaggacgtac tgccgccgcg gcggggcgag gccgtacggg acgaaatcgg
     6001 ttgtcacctc gcgttcgaat tcgaggaatt cgagttcggc caggttttgc agcatctcga
     6061 gcacaagcac cgagtccgcc gaggcgagga agggcgcgac gatctgccag gtgccgttgg
     6121 tttggcgttc gagggcgaaa gcaccatccg caatcccctc gatgcgcgtc acggcatccg
     6181 gatcgaacac catcatgcgg cggtcgcaga actcgcggaa actggccagc cacggcgcca
     6241 gactcgatcg ggccacggcg accacgttgg aatgctccat tcgccgcacg aaaagctggt
     6301 cgggcgcatt ggccggactc ctgccgattt ggagcgtcag aatgtcattg gttccctgac
     6361 tgagcaccaa ctcgcgatcc ggtggttgaa gaccgtaggg ctcgaggtcg gcccgcgggt
     6421 cgtcggtgac gaattgtatc actcgggcca gttcgagttg gtcgagcagg aggtccagac
     6481 gcgcgttgtt cgcccgtgtc gagagggggc gcaccatccg ccacacgtga ttcgaggggc
     6541 tccgcaccac ctcgaacccg ttggtgatcg ggcgcacctg cagccggtcg aatctgcgcc
     6601 tggccagccg caccagggcc gtgtcccgcc attcattcac cccgccgggg agggtcttga
     6661 gcaccgcgcc atcgacggtg tagagcccgc cgcgtccggc cacttgcgcg taaatctggc
     6721 cgcccagcgc cgtgggcgcg cccagccgca gatcgatggg gcggtcgcct tgctggatgg
     6781 cgatgctcac ggccggaggg gcgaagccga acgcggccag gccgttggtg tgcgccagga
     6841 gttcctgcgc cgtgacatgc ccctgccacc gcagatcccg caaggctgtc agcaaccgat
     6901 ccacaccggc cggttgcgcc ggatagcgga gcgggctctg gaattcccag ccgtcgtgca
     6961 cccgctcgag gcggatggcc ggattcgttg ccccggtcaa ggtcaacgcc gtcacctccg
     7021 ccgtcacgag ctcgggcaat agccgcgcgg cctgttcggc ccgacgcgca gtgtcgtccg
     7081 tgcggcgctc gaacagggcg atgaaggccg cgagggcaac ggccagaaac agcagccagc
     7141 gtgtggtttt tgcgttcatg cgagctagcg acgccgacgc caccagacca gcagcccgat
     7201 cagcagcggg gtcccgggaa ggacggccag cagaatccag cgcaactgga ccacttgaga
     7261 ctgcgacaga agaacactgt gctccttgat cgggcgaggg gcgagaccgg acaggtgttg
     7321 ggggcgatcc agcagccaat tcaccgccag actcgcaaac tcgatattgg cggccgattt
     7381 cacgatcgtt tcgttggcca gaaagatcga ttctcccacc acgacgagcc gcgtcgagtt
     7441 ccgatccgcc gccacgccct ggatgctccc cttctcgacc gcgaccgcca gcgggatcac
     7501 cccgcgccgg tctcgcgcgg ggttgaactc gggcagaccg ctcgacagtc cctcgctctc
     7561 cgtgcggcct cccacgctgg tgctcaccag cggctcgacc ttgggggcgt ctccagccgc
     7621 acctcccaag cgcagcggcg ccacggagcg ggggacgagg agatagaccg tgtgaccccg
     7681 catcggacgc acgatgggat ggttcgagaa attggtcgca agaatgccgc ctccgcggac
     7741 cgcgttgctc ggatcgagag ccaggttgtc gccgacagcc acaccccacc cggccaggaa
     7801 ccgttccaaa ccggtctggg catacccgga ctgatagaaa ctcagcagtg ccaacatccg
     7861 ccctccggtg tccagatagc ggccgatctt ctccagcgcg gtcgggtcga tgcggctgcg
     7921 cggcccggcc agcacgagca gttcgcagtc ctccggcacc tcggcgtcgc cgaccagccg
     7981 caacgtcgcc gtggcaatgt tcttctgctg gagcaggcgg gtgaattgcg agtagccgaa
     8041 ccgggtctcg agactggtcg gatcgtgctc cccgtggccc tggagaaagt agaccttggg
     8101 gctcctcccc tcgatcagat tggccagggc gtcggtgaag agcgattcgc ctttgaaacc
     8161 gacgcggcga acctccttcc cgcccgccag gagaccgctg atgtcgtact cggacagctc
     8221 gaccgcccgc acgatacggg tgcgtccctc gccttcaaag accaccaggt ccgagtcctc
     8281 gggcgacaac tggaggcgcg cggcggtcgt ctgcgccaac tgcacgtcgc ggtcgtagtc
     8341 gatggtctcg atcctcaggc gcggacacgc gtagccgtat tccttgagca ggcccgtcac
     8401 cggcgaatac agggtattgt tccgactgaa gagaatcgtt accttgacct cgttggcgat
     8461 cgctgccaac acctgccggg tggggaggga gagctgatac cgctcatcct ccgcccagag
     8521 gaaacgttgg aaatgacgcg cggcgagata attggccatt gcgagcaagg ccgcgagagc
     8581 caggagcgcc agggccacgt ttaacgcgat tcgaaagcgg ctggcggacg agaagctctc
     8641 ggttggatcg gagggatgcg gcatcttatt tccaacggcg gctctcgacc accttgacat
     8701 tgagaaacaa cacaaacgcc accaggctca ggtgaaagac cagcggacgc gtgtccacca
     8761 cgccgcgcac gaaatcctcc atgtgtccga acaacgatac atggttcacc accgtggcca
     8821 ggcagccggg ctgcgtgccg atgctgaatt ggaggaagct gaagagaaac agggcgagtc
     8881 ccgcgccaaa actcatcgtt gccgcgacga tctggctgcg ggtgagggcg gaggccaggc
     8941 agcccaaggc caggtagctg ctgccgatca agagaatgcc cccgaacgtg ctggcggtcg
     9001 tccaccactc gaaccgttcg ccgggcgagg cgtagtgatg caccagttgc agcacgccca
     9061 gcaacggcgc gatcgccacg gcaaagaaca tccaggccgc ggcgaacttg gccagaacca
     9121 cctggacatc gcccaccggg gtcgtcataa gcgtctcata cgtgcctgag aacttctcga
     9181 tggcaaacaa ccgcatcgtg atcaccggcg tcggcaccac gagcagaatc agccagaaaa
     9241 acggcgatcc gaaaaacaac tccgtcagcg gcgtgtggat ggtgtcattg ttgaccgctt
     9301 cgatgatgtc gacaaagctg acgccggtca ggaagagcat ggccgcaata atcagatagc
     9361 ctcccagccc gctgaagaag ctgctcagtt cccgccggat cagggtgatc aggacgcgca
     9421 tcagaggccc tcctcctcct cctcctcctc ccggtcggcg cgggtgacat ggacgaagat
     9481 gtcttcgagc gagtgccggc tgtgcgtcag ctcccgcagg cgccatcccc gcgccaacac
     9541 cgtctcgaaa atccgcacac gcagatccac tccgtgctgg ggcgtgagag cgcaacgaca
     9601 gtagagaccg tcggaaggcg tcacgtcgaa gcgctcgacc ccctccattc cgtcccagca
     9661 ggcggccagt tcgtgcgggt tggccgcaat ctccgccacc acctgtccgg agtcgctcat
     9721 ccggcgttgc aggttctccg tggtgtccgc ggccaggact cgtccgccgt gcagaatgag
     9781 gacccggtga cacgtcatct cgacttcggg gaggatatgc gacgagagga gaaccgtccg
     9841 gtgccgcccc agttccttga tcaactgccg cacagcgcgg acctggtgcg gatcgagccc
     9901 gatggtgggt tcgtcgagaa tgatgagatc gggctcatgg accagggcat cggcaagccc
     9961 cacccgctgc cggaaacctt tggacaacgt gccgatgatc ttccatccca cttcggacaa
    10021 tccgcattgg tccatcacga cgctgacccg atcccggctc tcggcgatgc ccagtccctt
    10081 gagtcgcgcc cggaacttca ggtactcgcg cacgcgcatg tccaggtgca gcgggttgtt
    10141 ctcgggcatg tacccgatgc gccgacgcac gctgtcggcc tcgcgaaaca cgtcaagacc
    10201 ggcaatccgg gcggtgccgg aggtcgccgg catgaaacag gccaggatgc gcatgatcgt
    10261 gctcttgccc gcgccgttcg gacccagcag acccacgatc tcgccccggc agacgctgaa
    10321 cgagatccga tcgatcgcgg catgtcgtcc ataccgcttc gtcagatctt cgatttcaat
    10381 catcggttcc tggttgctca tcgactgttc tgtcgctcga acacgggccc ggagcagccc
    10441 tgcaaaccgt gctattgaac ctgcaacgca atctcgaggc tctcccacgt caacaccatc
    10501 ccccggcggc gctgcaccag aacttcaagc ccgacggtgt cgcctttctt cttgccgtac
    10561 aagtattttg ctgactcgac gaccccttcc ggggcccggc catccacggc gacgataatg
    10621 gcgccgcgcg aaagccgtgc ctcggatgcc ggccctgtgc gatccacatc ggcgatcacc
    10681 aggcccgcat tgtccgccag ccccagccgc cccgacagct ccggtgagat ctcctggacg
    10741 gccaacccca gtttccggcg gatcagagcc gcgttgaaaa cggcgccggc gcgcaccatt
    10801 cgcagcgtca ccgaccgacg gtccgaaccg cgcctgaggg tcagcgcgat gtcccgctca
    10861 tccccggcgg cgctcagttc tttgatgaaa tcgaggaaac cgcgcgtcgc ccgtccgttg
    10921 attcccacaa tcatgtcgtc gggcttcaga ccggccgcct ccgcggggct cccttcctcg
    10981 agcgaagaga ccgccagcgt cgatccgccc ggtcgcaccc gggcaccaaa ccagtactgg
    11041 cgcagttcgg gcgacgcgat ttcgagcagg gcctcggtga tccgcttgat cggaatggcg
    11101 aatccgatgc cctggccctc gcgatacact gcaatgctca gaccgatcaa ttccccgcgc
    11161 aggttgatca ggggtccgcc gctgttgccc ggattgatcg cggcgtcggt ctgcagccaa
    11221 tcctcgatcc ccatcggcgt gttttcgagg gcggggcgcc ggctcttcga gctcaagatt
    11281 ccgcggctga ccgacccccc gaggccaaac ggattcccca gggccagcac ggtctcgccc
    11341 aggagcaaat cgtcatccgc cgcgaatcgc accgtccgga atcgctcgcc cggccttgcc
    11401 tcgatcttga gcatggcgat gtcgttgcgg ctgctttccc caacgagctt cgcctcgtat
    11461 tctcgagagc ccgcctcgtc gccgatccgc actcgaatcc gcgtggcccg gcgcacaacg
    11521 tggtcgttgg ttatcagata cccggcctcg tcgatgatca cccccgagcc aaggctgtat
    11581 tgcgttgtgg tgaacgggcg ccgcctgtgg taaggggccc agaattcctg caacaagtcc
    11641 tcgaaagggt cacgtacggc gacgatggtt tccgtgccga tgttgaccac cgccggcatg
    11701 acctgggcga ccgcctcgac ggcagaatcc aggcgcacgt cgaactcggc gccaaacgag
    11761 tccggcagcc agaggcccag ggcgcctgcc caaatggcca gcaccacccc cgcgccgccc
    11821 gcacacgcca gaaccgcgga tcgaactgaa ctcatgaaac aaacgacgtt tcgcatcgcg
    11881 ggatgttcaa tcgcaggcgg tgtcggctgc gcgattgcac cgcgccgtga cttctggcag
    11941 actatgcctt cacgtgctga ccccgtcaca gaaaaccgcg cgcgaaatcg agtgcctgat
    12001 ccgggcccgt taccccatcc tctacctggt ttcgagcgag gaggcccgcg tcgagtcggt
    12061 gatctgcggg atcgcccggc gctgggagaa acaggtgtac caatggagct gcacgtcggg
    12121 cctggtcccg gccggcgtgt cggtgcaatc gcaaaagcag cgccattccg ccaccaaaga
    12181 cccgctggcg gcgctcgatc aggtggtcga tcaggtcgag cccgcgctgt tcatattcaa
    12241 ggatctccac ccgttcctgg tccggtcgaa ctacgccgtc attcgccggc tcaaggaaat
    12301 tgcgcttcac ctcaagaaca gtctcaagac cattattctc atctcgcccg tcctcgaagt
    12361 gccgatcgag atcgacaagg aaatcacggt gctccccttc ccgttgccgg gctgtgacga
    12421 gttcggcgag ttgctcgacc aggtcatagc cgaggtggcc ccgaccggcc gggtcgcgct
    12481 cgacctggac ggggcgggac gggagcggct gctgcgggcg acgctggggc tgaccctgag
    12541 cgaaggcgag aacgtgtttg cgaagattct gatcaaacac gagcggctca gcggggacct
    12601 cgtggccgaa gttctcgagg agaagcagca gatcatccgc aagagcggct tgctcgaata
    12661 ctacgccacc gaggagacgt tcgacacggt cggcggcctc gcccaggtca aggactggct
    12721 cgccaaacgg gcgagcgccc tctccgatgc cgcccgcgac tttggcctgc ccgctcccaa
    12781 gggcatgctc ctgctcggcg tccagggctg cggcaagagc ctgtgcgcca aggccgtctc
    12841 caacgcctgg caggtccccc tcctgcgatt cgatatcgga cgtgtattcg gcagcctggt
    12901 cggatcctcc gaggaaaacg tccggcgcgc catcgcggtc gccgaatcgg tcgcccccgc
    12961 cgtcctctgg gtcgacgaaa tcgacaaggc ctttgcgggc tcccagtggt ccgccttggc
    13021 ggatggcggc acgactgcgc gcgtgttcgg cacgttcctc acctggctct ccgagaagac
    13081 cgtcccggtc tttgtcctgg ccaccgccaa cgacatttcg caactcccgc ccgagctgct
    13141 gcgcaagggt cgcctcgatg agattttttt tgtcgatctt cccgatgcag ccgagcgact
    13201 cgagatctta tccattcacc tgcgcaagcg aaatcgggac ccggccgggt tcgacctcga
    13261 cgccctgacg cgcgcctcgg ccggctatag cggggccgag atcgaggagg cggtcgtcag
    13321 cgctcttttc gacgcgttcc atgcccgaca acagctcgag accggccaca tcctgaccgc
    13381 cctcgaacag agtgtgcctc tcgcccgcac gatgtccgag cagatggacc ggctgcgcca
    13441 gtgggcggtg ggtcgggccc gaaacgcgcg ctaatccgca tattttaccc ccgtcagtgc
    13501 acagggtggc cggatctcgc ctgatgtctg ttatgcgggt tagaaaggca aaaccgcgcc
    13561 aggtcccaga gaccgagaac tgcaacacac tgatctcacg gcagaccact ctcagaaccg
    13621 acagaatgct cccggccccc tttctgagcg cggttttgcc ggagctcgga catcgaactc
    13681 cgcgaagcct tatgaagcac tcgggatcgg gttcaaaatg gccggttgcg gtctcgaatc
    13741 gccgagcatg taacggttgc ggtcccttgc ccggttggta gaatgcgcgt ccggcaggca
    13801 cgggggaggg taagggtggc gcggtgcccc cgatgggcct cggagtcgga acgatttgtc
    13861 ggatcgattg cagaaaatgc aggtgatttc ctcagtcaat cattgtcggt tgcgccggtc
    13921 cgatacgccg tgaaggccgt gcttcaaacg agcgtgctcg tggccgccct gagcgctttg
    13981 tcgcttctgg gcgccgccga ccagccgcgt ccgctccgcc tggggcattt cccgaacctc
    14041 acgcacgccc aggccgtcta tgcgcgcgcc ggcgggcagt tcgatcgcaa gatcggtcgg
    14101 gcggttgagt ggacctcgtt caacgctggg cccacagcga tcgaagcgct gttcacggac
    14161 gcgattgatg tcgcgtttgt cggtccaaac ccgaccatta atggattcct tcgcacccgc
    14221 ggggagaaat tcgtggtgat cgccggcggg gccagcgggg gaacgggcct ggtggtgcgt
    14281 tcgggggcgg gcatccggag cgaggcggac ttcgacggca agatcattgc cacgccgcaa
    14341 ctgggcaaca cccaggatgt ggcggcgcgg ctctggtttg ccgagcacgg gtacaggctc
    14401 aaggaaaagg gcggcagcct gacgctcgtg gcgctgtcga atccggatca actcacgctg
    14461 ttcaggagag gcgagatcca cggggcctgg accgtggagc cgtgggtggc gcggctcgaa
    14521 ctcgagggcg gcggcgagct gtttctggat gaacggaccc tttggcccga ggggcggtac
    14581 gtgaccacgc atctggtggc caatcgagtt ttccttcgag agcaccccca gttgattcgg
    14641 gatttgctgg ccgcattgat cgaggtgacg caggagatca acggggacaa gaatgcggcg
    14701 gcccgcatcc tgaacgccga gttgaaacgg gaaaccggga aggcgctccc cgaggcggtc
    14761 gtcaaacgcg ccttgcagcg gatcgagttc acctgggacc ccatcagcgc ctccctgcgc
    14821 aagtgcggcg aggccgccta ccgcgtcggc ttcctcagga gccagccgcg tctggcgggc
    14881 ctctactcgc ttgatgcctt gaacgacgtg cttcacgcca agggtctccc gcccgttgcc
    14941 gattagccat gcagccacca gccgctcccg accacccgcc ttccgccgag ccggcgcccg
    15001 cgccgccggg ggtgaagctg cggctcgcca acgtcagcaa ggtctttcgc aacaacgagc
    15061 agacgatcga ggcgctcgcg cccgtcagcc tcgagatccg cgagggcgag ttcgtggtgc
    15121 tcttcggtcc ctcgggctgc ggcaagtcca cactgctcaa tctcatcgcc gggttcgacg
    15181 cgccgaccac gggcgagatc acgctcgaag gccggcgtgt ccggcgcccc ggcaacgacc
    15241 gcctcatgat gttccaggag cacgccctgt tcccctggct gaacgtcctc gacaacgtca
    15301 cctacgggct gcgatggctg ccgaatttcc ggtttcaccc cctgcggcgg cggcgccgcg
    15361 ccatggagtt gctcaagctg gtgcgcctcg acgagttcgc tcacgcctcg atccacgaac
    15421 tgtccggcgg catgaagcag cgcgccgccc tggcgcgggc gctggccccc gaccccaaac
    15481 tcctgcttat cgacgaacct tttccggcgc tcgacgccct ggtgcgcgcc aagctgtacg
    15541 gcgagctgca ggacatcctc gtccgcactc gcaagacgat cgtgtccgtc acccacgatt
    15601 cgcgcgaggc cgcctgcctg ggcgatcgcg tggtcgtgtt caccaagcgt cccgggcgca
    15661 tcaaggcgga gatcaaggtg gacatgccgc gtccgcgcga catcaacgcc gccgccgtca
    15721 ccgcctacgc cgcgcaaatc gccgcccaac tcgaggagga accgcgtgag aacgacgacg
    15781 gaaaaaggga ctaagcgcta cgtcgcgggg ttctacctgc tcctgttggg tggctggcag
    15841 acgctcttca gcctccaact gattcccgat tacctgttcc cctcgcccct tcaggtcggg
    15901 gaacgcctct gggaactggg ccgcgacggt ctgctctggc ccagcgtgtc ggccacgctc
    15961 atgcgcatga ccatcggctt ctccgccgcc gcgggtctgg gcctggttgt cggcgtcgtc
    16021 atgggcatga accacgtcct gaaccagtgc ttgcggtcgc tctttctcgg gctgcagacg
    16081 ctccccacgg ccgcctgggt cccgatttcc ctcctgctct tcggcctgag cgatcgcggc
    16141 atctatttcg tcatcgtcat gagttcggtc gccgcggtcg ccatcgcgac ctcggacggc
    16201 atcctgcaca ttccgcccct gcacctgcgg gcggcccgca ctctgggcac ccctggttac
    16261 gccatgccgc tgcgcgtgat cctgcccgcg gcgctccctg gcatcgtcac tggcgtgaag
    16321 ctggggtgga ccctcggctg gcacggggcc gtctcggccg agttgatcaa gtcgagtgtc
    16381 ggcttgggtt acctgctcta catgggacgg gaactcaacg acgtcgccca ggtcctgggc
    16441 atcatgatgc tcaccatctt gttcggcctg ttgctcgacc gctttctctt cggccagatc
    16501 gaacgccgca tccgcacgcg ctggggcctc accagccagt cctgaacccg ccgggacgaa
    16561 tcgggtgttg acgacccaag ccgcctcggg tcaggcttct cctcacctga agctcaaatg
    16621 cacccgctcg atccgcgcgc tatggcagca aatatccaaa cccctgccac gactcctcca
    16681 ggcgccccct ccgggcacct gacgacttat ctcctcggca gcgccgtggt tgccgctctg
    16741 ggcggcctgc tgttcgggtt cgagacggcc gtgatctcgg ggacgacgga gtggctccag
    16801 aaacacttcg agctcaccga tttcatgctc ggcttcacag tctcgagcgc gctgatcggc
    16861 accatcgtcg gcgccatcag cgtcgggcgc ccggccgacg catgggggcg ccgcggcgtg
    16921 ctcttcattc tgggaatcct gtttctcctg gcggccctgg gctgcgcctt cgcgtggaac
    16981 tggcacgcgt tcatgttctt ccggttcctt ggcggattgg gcgtgggcgg ggcgtcggtg
    17041 gtttcgccaa tgtacatcgc cgagatctcg ccggcatcgt atcgcggacg actggtggcg
    17101 gtgactcagt tcaacatcgt gttggggatt ttactggcct atctgtccaa ttacatcatc
    17161 ggcagcctgg gcctgggcga tgcgcagagc cgctggatgt tcggcgtcat ggccctgccg
    17221 gcgatcgtct tcctgctgct cctggtcttc acaccccaga gcccgcgctg gctcgtcgcc
    17281 cagggacgca tcgccgaggc ccgccgggtg ctcgagaaat gcgggacaga cttgaatgcg
    17341 ctcgacttcg agctgggcga aatccaggcg tctctcgacc tcgcgcatca cggtctcgag
    17401 gagccgtttt tctgccgtcg ctacctcaag ccgatcctcc tggcggtggc gatcgccatg
    17461 ttcaaccagc tctcgggcat caatgccctc atctactata ccgcccatat cttcaagatg
    17521 gccggagccg atcaggcgtc ggccttgctc caatcggtca ttatcggctt caccaacctg
    17581 gtcttcaccc tggcggcact gaccgtgatc gatcacttcg gcaggcgcag gctcatgctg
    17641 ttaggctcga tcgggtacat cgcgagcctc agcgcctccg cctacgcctt ctacacccac
    17701 tccggcggct ggctgctgct gggcagcctg ctcgtcttca ttgcatccca cgccttcggc
    17761 cagggcgccg tcatctgggt gttcatcagc gagattttcc ccaatcgcgt ccgcgcccgc
    17821 ggtcaggcgc ttggcagctt cacccactgg atcatggccg ccgccatctc ctggaccttc
    17881 cccatgatcg ccgcgaagtc gggcggacac gtcttcgcct tctacgcgct ttgcatggtg
    17941 ggccagttga tctgggtcat ccgtgtcatg cccgagacaa aaggaatctc gctcgaacaa
    18001 atccagaagc ggctggggat cgagtgacgc cgcccgtccc gccgcccttc cacacacgcc
    18061 aacgcatctc ttatgaaaac tcaaacccgc atcccggcgc tggtcgcact gctgctgtcg
    18121 agtctcccct gcgccgcggc cgacctttgg aatgtccgcg atttcggcgc caaaggcgat
    18181 gggtcgaccg acgacaccgc cgctttccaa caggcgctcg atgcggctgc aaaggccggc
    18241 gggggcacgg tgagcgcacc ccgcggcaat tatcttttcg ccgggcactt gaacgtcccg
    18301 acggcggtga cgctcagagg cgtctgggaa tctgtcccgg cccacaatgg gctgcgcgac
    18361 cgcggactgc ccaaaccgac cgatgacggc accaccttcc tggtcacgga gaacgccggc
    18421 aaggaaggcg ggctgccttt catcacgctc aaccataaca gcaccctcaa aggcgtcgcc
    18481 ctctattacc ccaaccagaa agcggatgat atccccgtgc cctacccctg ggccgttgct
    18541 ctgcgcggca agaacccggc cgtgctggcg gtcgaactcc tcaaccctta caacggcatc
    18601 gacgccaccc agaacgagcg ccacctggtt cgcgacgtgc acggccaacc cctgcgccgc
    18661 gggattctcg tcgaccggat tttcgacatc ggccgcatcg agaacgtcca tttcaacccg
    18721 tggtggagca cgagaccgaa gctcttccag tggcagatgg agaacggcga ggcgttcgtc
    18781 ttcgggcgca gcgactggca atacgtcctc aataccttct gtttcggcta caagatcggc
    18841 taccggttca tcaagtccga gagcggcgtc tgcaacggca acttcctcgg cctgggcgcc
    18901 gacgactgcc acacctcgct cgtggtggat gaatcggcgc cgttcggcct cctgatcacc
    18961 aatggcgagt tcgtctcgtt ccatggtccc gatccgacga tggtcgaagt catgggcacg
    19021 catacgggcg ccgtgcgctt cgtcaattgc gccttctggg gtccgtgcca tcagatcgcc
    19081 aagatcgacg gacgcggcac ggtgggcttc agcgattgca ctttcgtcca atgggattcg
    19141 aaaagggaag ggcgtcacgc catccgggcg aacggcggca cgctcctggt gcgcggatgc
    19201 gagttccgcg aagccaagcc ccagattcac ctggccgaag gggtccgccg cgcggtgatc
    19261 accgacaacg tcttcaacgg cgccgcgcga atcgccaacc tcagcacggg gtccgtccag
    19321 atcgccaaca acgccggcga caaagggcac tagcccgtcg gaccagatca tcggagggac
    19381 aatgaacctg ccctcggttc tattgctgat tccggcctac aacgaggagg gccgaatcgg
    19441 ccccacgctg cgccagtacg catcttatgt gcgggatcac tacgcggggc gattcgagat
    19501 tgtggctgtg gtcaacggat gtcgggacaa cacgctcggg gtggtcgaga gtgtggctcg
    19561 cgatttttcg aacatccgct ggctggtcat tcccgatcgt gtgggcaagg gcggcgcgct
    19621 gattgaagga ctgcgcctgg caccggaagc ggacatcatc ggatacgccg acgccgacgc
    19681 ggccacgtcg cctgcctcgc tgctcgagtt ggcggctcgg tgccgggact acgactgcgc
    19741 ggtcggctcg cgacgggtgc cgggcgccgt catccaccag ttgcagccca gccaccgcca
    19801 actggccagc aagattttcc atttcatcgt cgagttcctg ttcgggatgg ggattcacga
    19861 cacccagtgc ggagcgaaat tcctgcgcca acacatggct ttgaaaatcc aggatcgcct
    19921 gctcatcgca gacatggcgt tcgatgtgaa cctgctctat gccgtcaagc acgcaggcgg
    19981 caccatgatc gaggtgccgg tggattggac cgaccacctc ggttccaccg tccgatactt
    20041 ccgcacctcg ctggtcatgt tcctgtccgt tgtccgactc cggctcgtct actcgccctt
    20101 ttactcctgg ctggggccaa tccgccctct cgaagcctgg atatacaagg cgttgcgcaa
    20161 tccgcctccg ctcgccgtgc ctccgcctga ccgtccggca acaccaacac caccggagcg
    20221 gtgaggcgtg agaggacgcg gccggcggat ttccggcaga tgtggccggc gcgaagcgac
    20281 cgggcacgcc ggctcatgga gtgggcggtg gaggcgccgc aggggtgtgg gtctgttgcc
    20341 aacgcgcgcg aagcgcggca tgcagttggt tccgaaaggc gaggggcaat tcgtacggct
    20401 ctcccgcctt gtaaagcgtg atcgtcttgc gaatgttgtc cacgacaacc tggcagggat
    20461 tgcatccggc aagatgccgc tcgaactcga cacacaaccc ggcggcggtc tcgccgtcga
    20521 catactcatt caagagagac agcagctcgt cacagttcat aaacggcttc tatcaggagc
    20581 catcatcggg cagaaggtta cacagccggt gctgcgtcct catcgtgccg atgcggtgcg
    20641 agccgccggg ccgggtctcc aaacaccttc gtcaaccgtt cccgcaactg gagccgggcg
    20701 cggagcagcc ggaccttcac gttgacctcg ctgatcccga tgaactcggc cgtctcccgc
    20761 accgagaacc cctcgacatc ccgcagcagg aacaccaggc gatgcttctc atccagctcc
    20821 gccagagccg cttcaatgag ttcgcgtgtt tcgttgcgct cgaccagtct ctccgggctc
    20881 tgccgccagt cggcgatgta ctccggatga gggatcgcct cgccccccgc ggcagggtcc
    20941 acggcgtggt cgaacgacac caggtcgagc ccctcgcgtt tgcggataac tttcaaagcg
    21001 gcatgcgtgg caatgcgcgc caaccacgtc gcgaacgaag cttccccccg aaagcccgcc
    21061 aggttctcga gcgcgctcag aaaagccgtc tgcgtcacat cctcggcgtc ctgttcctgg
    21121 cgcagaatgc gcagggcaag cgagtagatg tgacgttcgt gccgattcac cagcagctcg
    21181 aaagcagcca gttcgcccgc ctgggcgcgg cgcaccaact cgatgtccgc gggtgaatcg
    21241 cccgcctcgc tcggtgagga cttgggccgt ggctcttccg gtgtcacaga ggattgaaac
    21301 ccaacacgct cgaatccggg ccgtcaagct ttctgccaga gcccgggagt cagcccccac
    21361 ggttatttca tttaaattcc tgaagggttg ctgttgttga ggttggaaa
//
LOCUS       DAOQRO010000007        20754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1753_length_20754_cov_5.167786, whole genome shotgun sequence.
ACCESSION   DAOQRO010000007 DAOQRO010000000
VERSION     DAOQRO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1753_length_20754_cov_5.167786"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PKK20_00580"
     CDS             complement(<1..1693)
                     /locus_tag="PKK20_00580"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNU98418.1"
                     /translation="MRLEFSPDNSLNGKAGPERPVWLRGVRTGENLVLVFVLAAMVLL
                     PCAEIVLRKLFNTGIPASAPIVQHLVLVLGMLGGAVAARDNRLLALSTLSAHFRGRFK
                     EVALVFSSGFAAAMSFLLCKASMDFVLASRRLGKTLAYGVPLWIVQLVLCIGFALVAW
                     RMIRHAGTTWRGRVGAFALAAAVVAMGAWAPIPPEQLRIPALLTLVIAVAVGAPIFTA
                     LGGAGLILLWSAGETIASMPINHYRLTVNPTLPSIPLFTLAGYFLAEGGTARRLVKVF
                     DALFGHLRGGAAIGTVLACAFFTSFTGASGVTILALGGLLMPILLSAGYKERTSLGLL
                     SGAGSLGLLFPPCLPPILYAIIASSTGSSIGIEEIFQAGLLPGLLLVVMTGVWGIVKG
                     PHVQAERRRFNLRVALDAMWGAKWELLTPVVALTALFGGFATPVEAAALTALYAFFAE
                     VVIYRDLKVFKDVPRVMTECGCLIGGVLLILGVALGFTHFLIDAQIPDQAVAWVTANI
                     KSRWLFLLALNLVLLVVGGVIEIYAAIVVVVPLLVPVGVAFGIDPLHLGIIFLANM"
     gene            complement(1701..2732)
                     /gene="dctP"
                     /locus_tag="PKK20_00585"
     CDS             complement(1701..2732)
                     /gene="dctP"
                     /locus_tag="PKK20_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNU98419.1"
                     /translation="MNRHAPRQMSRWFLGLAGSLWLACAGATLGAEKIKLATLAPTGS
                     SFHKSLLAMKDAWRKASNGGVDLVIYADGKLGGEADTVGLMSVNSIQAAMLTGVGLAE
                     VEFGVAGLQNIPMGFRDLAEVDYACGKLEPMLEKRLLEKGFVVLFWSDAGWVRFFSKE
                     PVTYPEDLKKLKLFTWAGNPEQVVIYKSGGFNPVPLETADIVPGLQTGLIEATPAPPV
                     FALASQIDGRAPHMLELNWAPLIGACIVRRQAWEKIPADTRAKLLEAAAAAGKEIKAN
                     GRRESAESVAAMEKRGLKVTRVTPEIEAAWRAEAEAVYPKIRGKLVPADVFDETLRFI
                     REYRANQGK"
     gene            complement(2780..3742)
                     /locus_tag="PKK20_00590"
     CDS             complement(2780..3742)
                     /locus_tag="PKK20_00590"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNU98420.1"
                     /translation="MTTMQTKAGGRWGRWLRVALAGLLLLLPGCSIKRMAINRVGDAL
                     AGGGATFSSDDDPELVGAALPFSLKLMESLLAESPRHRGLLFATASGFTQYAYAFVQQ
                     EADEAEDQDLAKAQAMRARARRLYLRARDYGLRGLETAHRGFAAGLQAQPIEAVRAVG
                     KADVPLLYWTATSWAAAISMSKDDPGLIAEIPQMEALIDRALVLDEAFDKGAIHSFLI
                     AYEMARSGGLGDPAERSRQHLERALALSGGLLAGPLVSFAEAVCVQKQDLKEFDILLE
                     RALAIDPDAQPEMRLVNLIMQRRARWLMSKRDELFLILDQPSDG"
     gene            4225..5856
                     /locus_tag="PKK20_00595"
     CDS             4225..5856
                     /locus_tag="PKK20_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNU98421.1"
                     /translation="MPEILLATLNAKHIHSALGLRCLMANLGPLRERAALLEFDLAQR
                     PLEIVEQMLAVSPRIVGLGVYIWNVAPITEVVELLRRLRPGVVIVLGGPEVICETDLP
                     LVAGLADWVIAGEADLEFAALCRDILAAPPPAGGADAGGPFGAAERPCPSPGMARKPR
                     IIRAGHARLADLALPYEFYSDTDIAHRIVYVEASRGCPFACEFCLSSLEALVRQFDVN
                     LFLGAMERLLERGVRHFKFVDRTFNLNIEVCVRILEFFLARLRDGLFLHFEMVPDRLP
                     VELREVLARFPAGTLQIELGIQTFNDEVAHRINRRQDCARIAENLRFLRQHTRAHLHT
                     DLIIGLPGERIESIADGFDRLIALGPHEIQMSVLKRLRGVRIIRHDDAWGMVYSPLPP
                     YELIESRTLDFPTLQRLRRFARYWDLIGNSGNFVETTLLLWAKGGESPFAGFLRLSDW
                     LFAQTGRQHAIALPRLAELAFEYLTRVLGCDPTVVAASVLRDYRRSGRSGDLAFLRED
                     VDRKTKAHRAAGPRRSDRRQARHRAADDQSRRTAR"
     gene            complement(5832..11531)
                     /locus_tag="PKK20_00600"
     CDS             complement(5832..11531)
                     /locus_tag="PKK20_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU98422.1"
                     /translation="MPRTVRNRPKASSWLLRAWGWAALGLVLVWVLGHAGAQPPVHSV
                     HLPDAAPPAAGASPWLEGHADLEASVPTTRVVPNQFVVRFRDAAAFAHPASAGVDLGP
                     GIKSIRWLSGSGAARAGRTSPGTSVFDRIALVTAAAGESADLVLARLQQHPALAYAEP
                     NTILTLFDDPHPARYPNDFRFDELWNLHNPDAAAGRPAGDIRAPFAWALTTGDAAIRV
                     AVIDTGIDFYHPDLEANVWTNPGEIPGNGLDDDGNGYVDDVHGYDFVSDDSDPMDDQI
                     HGTHVAGTIGAVGNNEIGVTGVCWQTSLMAIKAFNASGEGSLFDVIAGIRYAVVNGAR
                     ILNASWGQFENSKALAEAIAQARAQGMVIVAAAGNTRSDALSYPAAYPEVLTVAALDS
                     RDERSIFSSYGPHVDLSAPGQNILSTVPDNRFEVASGTSMAAPHVAGVAALVLSRHPE
                     FTPVQVEDILRNAVDPIQTAVYCGAGRLNAWRAVRVDVPLPTARLELPPVVSGRIDLE
                     GTASSPRFRGYALDYGQGVYPTNWTRLHAAELPVDRGALLADWSTDSLPEGSYVLRLT
                     VWDTLDQQAVDRAMVTIRNVHLASPLHTDVVRAGSILPLTGTVYGTGRTYTIAHGIGR
                     DPSAWSTDGIVLANGGQAPVIGSLLGTWDTGAVAPNTFYSLKLTAWAGGETIGSWTAT
                     LVHLDGQLRPGWPQHLIVPSGYSTNDWRQIVVADLDQDGAQEILTVEPGNLEGKMARL
                     LVYRPDGALGWSRALAAGFPTTDLPTVGDIDGDGMSEVFVDVGDSGRIWAFRHDGRPL
                     DGDWPALAGGGNFGKVLADLDGDGTAELVVYGTSSAAHPTGRRMLAVLDRWGRIRQQW
                     FMADAALPLEVPRQFPAVADLDDDPDLEIVAIAGEGGVAMFDLRQPEGPVWQAQTGGF
                     LYGSPVVGDIDGDHRAEVIVASHNASARGKTGTRGGVFVYDREGQIRPGWPVLIHESF
                     TATPALGDLDHDGALDIVAVNWEEKLIHLLRYDGFDHPGWPVGPILANDASLFPMLGS
                     VKSMPVVGDVNGDGWPDVVVITPGYPNQAAATGDISYFGGVKAWSRAGKPIDLHPHPH
                     VTALVTETGAGNRFKAGPPVLTDLDGNGRLDIVASTIEDYGYGGRAAANVAKGRYSIY
                     AWELDAPHVPDDLPWPMFQRDPAHTGSVSVLKPANQPPAVEGIPSQTIRAGGVFFPIE
                     LDRYVLDPDHPVAALTWTIEGASDLLVALEDNRVVAIEPPTPEWTGAETLRFVAHDPG
                     GLVGSAVATFAVRLDYVPPLAVADVVELDEDTACTLNPLANDSHPRGLPLRLLQFSRA
                     RAGSIEALGNGLLRYQPEPDFFGEDSFAYLVDDGQDGMALAWVTLRVRPVPDPPVAVE
                     DRVITIENTPIAIDVLANDTDADGDVLALTGFGQPANGAVEQGPGASLVYRPRQGWSG
                     IDSFGYSITDGQGHETSGTVVVQVKPVNDPPVAQDQVLVLNRNTSRDVIYQAVDPDGD
                     ALSFEIVKGPDHGEFWAQPTVATYYPRFGFAGEDTVTYIARDGELASSPAVIRFVVLA
                     VNNPPIAAPQSLTTKVGRPIELTLDATDADADPLSFEIARQPAHGTVTVEGSNALYAP
                     RLGYLGDDSFTFTAHDGQASSSPGIVSLRMTDQNTAPIAESYRLAVPMNRPTNVVLRA
                     IDGENDPLQFTIVTNPAHGRFSGTAPNLIFTPHTNYYGPDRFAFTVHDGELQSAPGVV
                     ALSIVHPNTRPETTNQWLTAPLDAPVSFLLEVTDSDTNVLRAVILEGPRHGRVHGLGT
                     NFVYTPRSGFTGSDSLTYRVWDGIAYSPIATVSINVQTTPPPTPPRFEALKLIDGGML
                     QMRIATMPGRLLRLEVSTDLVSWEPLLSQTPSTDTVALVDTNAPSLPRRFYRAVRLD"
     gene            11904..12148
                     /locus_tag="PKK20_00605"
                     /pseudo
     CDS             11904..12148
                     /locus_tag="PKK20_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649603.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            12353..13534
                     /locus_tag="PKK20_00610"
     CDS             12353..13534
                     /locus_tag="PKK20_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNU98423.1"
                     /translation="MSTRGSAGGFWGLKAGVAFLNHGSFGACPKPILELQAELRRQME
                     AEPVQFLWRHYDERIEPSRQALARFVGARPSDLVFVTNATTGVNAVVRSLNLRRGDAL
                     LTTCLDYNACRNVLVEAARRAGARLIVVPVPFPVSGSDAIVAAVLKAVTPRTRLALLD
                     HVTSDSALVLPLERLVRTLEDRGVPTLVDGAHAPGMVALNLARLRPAYYTGNLHKWVC
                     APKGAAFLWAREDLQSGLQPPVVSHGNNRSRPGHTRFQDRFDWPGTIDPTPWMCVGEA
                     IRWMGSLLPGGWPALRARNHALAVQARALLCRSWRVDSPCPASMLGSMATVPLPARLQ
                     NRPPAGKIDADQLRLYDEFGIEVPFNRIGQPPIRYLRVSAQIYNTVAQYRRLNRAVQM
                     L"
     gene            13894..14583
                     /locus_tag="PKK20_00615"
     CDS             13894..14583
                     /locus_tag="PKK20_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98424.1"
                     /translation="MHDKEITTYLANVLYIAIQDGRLTSQELAALDEVRRLVGAERIH
                     ATEATRLVENSKFIPQGVGTFGAQVRNLEDMVFVGLADADLVAGEREAIEVFCEQIGL
                     TQTQLDQIVREAQVRCSDLELRAAPGSDATESKSDAHDNGRSAGTDGAQTWHPRQTTI
                     ARRACKTSIEDCRAAGIKSVELLPSNRDGEQCAMALSMRGTPLDIDRLPELPLPYCTA
                     RRCQCVWIAAG"
     gene            14638..16104
                     /gene="selA"
                     /locus_tag="PKK20_00620"
     CDS             14638..16104
                     /gene="selA"
                     /locus_tag="PKK20_00620"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNU98425.1"
                     /translation="MRKKPRERQPESQRRAIPAVEQVLQALSDVGLSRPVVVAVVRRE
                     LGRWRKGGAVPAFETVVDGIRNALETLRRSALRPVINATGVVIHTNLGRAPLGPAAIE
                     ALTAIGANYSNLEIDLASGERGRRAAYVEQLLAVLCGAEAATVVNNCTAALVLMLRHF
                     TSGARKEVILSRGELVQIGGGFRIPDVLETSGATLREVGTTNQTTLADYADAIGEKTA
                     LVLKVHRSNFFMEGFVDSPSIEALAELARRKRLPLIEDLGSGAVLNTTSYGVDREPTP
                     RESLRRGVGLVCFSGDKLLGGPQAGVIAGKARWVAALKREPFFRALRCDKLILAALEA
                     TAEAYARASRVGRGSSPPIAPAGLGRQIPVFGMLETTEETLHARAHALQTELSGLPLS
                     ITVGEGRGRVGGGTLPRAALPSVTLDLSPQTVAPAQFLARLRSGSPPVIGFLSGGRVR
                     LDLRTVSAGQDGLLAEAIRIAACGMAPDRVEPSRRTAC"
     gene            16419..16844
                     /gene="msrB"
                     /locus_tag="PKK20_00625"
                     /pseudo
     CDS             16419..16844
                     /gene="msrB"
                     /locus_tag="PKK20_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
     gene            17294..18580
                     /locus_tag="PKK20_00630"
     CDS             17294..18580
                     /locus_tag="PKK20_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU98426.1"
                     /translation="MNPVIALAVLSLGAAAVQADWPEFRGPWGNGHAAGSPDGKTVGV
                     PLHWSETNNVRWKTAIPHRGWSTPVVMGGQVWVTTATEDGHDFFVLGLDADTGKVVVH
                     EKVFHSDNPEPLGNGASMNCYATPSSLIEPGRVYLHFGSFGTACLETGTGKVLWKRDD
                     LPCRHYRGPSSSLISFENLLILTMDGVDVQYHVALDKQTGKTVWKTGRSVAWNDEHVP
                     GQMARDGDLRKAHSTPLIVLAAGQTQMLSAGAKAAYAYDPRDGRELWRVQYNDWSAAP
                     RPLYDNGLAFFVTGLSKTELLAVKTDGQGDVTDTGVVWRLRTHVGKYSSPILVDGLIY
                     TAAEDSFVTCLEAATGNTVWKERIGGKYAASPVYADGRLYFFNQQGTTTVLEPGRAFR
                     VLATNTLDDGFMASPAVSGRAFYLRTKTHLYRVESP"
     gene            complement(18659..19876)
                     /locus_tag="PKK20_00635"
     CDS             complement(18659..19876)
                     /locus_tag="PKK20_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98427.1"
                     /translation="MKQQRMRRRDFLGSAALGTAGIVWGDRLTALASGASPFRINEPF
                     DGAVLNRRHGKAVDGGLAILVSGVAPADARVMVCGRDAERQGETFSAEVVLREYETTI
                     TATCAGGSGRLEDRVRVVWDRHSFPRYRFSIDDNSFFLRDIHQKQYRSLFDSFYLAML
                     RKLHRDYGVKFSVNIYYTTADGFDLTQLSDRYKGEWRDCADWLKLAFHAHADKPDRPY
                     EDVSPEKLIADFDQVAEQIHRFAGAQSYAPPTVIHWGMTRQESLKPLFERGVRALSGY
                     FVKWSGRYDVNYRLDDTVSEYLSRHDCWKHFDSGIVFSRVDIVCNGTPLDQIAPTLEP
                     LAKDPNQAEIMDLFTHEQYFWPFYANYIADHPQRLDAAIRWVTEHGYRPVFLHEGFLG
                     APTPSIETPTRPG"
     gene            complement(20147..>20754)
                     /locus_tag="PKK20_00640"
     CDS             complement(20147..>20754)
                     /locus_tag="PKK20_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNU98428.1"
                     /translation="LASGHWKYFNTPRDSFWCCTGTGLENHATYGDTICFHNDRTLWV
                     NQFIASELTWRERGVTVRQDTTFPDTSATKLTIRATAPTEFELRMRVPQWATEGVTLK
                     IDGQDQAVTAQNGSFLSIRRTWRDETTVEMQMPMCLWLCPMPDDPDLVAVMYGPLVLA
                     GELGKVDVPRDLVYATGNWFEYELEVLPEVPMTLHCTYCCL"
BASE COUNT         3615 a         6689 c         6819 g         3631 t
ORIGIN      
        1 ccatgttggc gaggaagatg attccgaggt gcagagggtc gatgccgaag gcgacgccca
       61 ccggcacgag caggggaacc accaccacga tggcggcgta aatctcgatc acgcccccta
      121 ccacgaggag cacgaggttg agggcaagca ggaacagcca gcgtgacttg atgttggcag
      181 tgacccaggc caccgcctgg tcgggaatct gggcgtcgat gaggaaatgc gtgaaaccga
      241 gggctacgcc gaggatgagg agtactccgc cgatcagaca gccgcattcg gtcatgacac
      301 gcggaacgtc tttgaaaacc ttgaggtcac gatagatgac aacctcggcg aagaaggcgt
      361 agagggccgt gagggccgcc gcctcgaccg gcgttgcaaa gccgccgaag agggcggtca
      421 gagccacgac gggcgtcagc agttcccatt tggcgcccca catggcgtcg agcgcgacgc
      481 gcaggttgaa gcggcgtcgt tccgcctgga catggggtcc tttgactatg ccccagacgc
      541 ccgtcatcac caccagaaga aggcccggca gcagtccggc ctggaatatc tcctcgatgc
      601 cgatgctgct gccggtgctg ctcgcgatga tggcatagag gatcggcggc aggcacgggg
      661 gaaagagcag accgagcgat cctgccccgc tgaggagtcc caatgatgtc cgttctttgt
      721 agcccgccga gaggaggatg ggcatcagca ggccgcccag ggcgaggatg gtcactccgg
      781 aggccccggt gaagctggtg aaaaaggcgc aggccagcac cgtgccgatc gccgcgcccc
      841 cgcgcaggtg tccgaagagg gcgtcgaaga ccttgacgag ccgacgggcc gtgccgccct
      901 cggccaggaa atagccggcg agcgtgaaca gcggaatgct cggcagggtc gggttgacag
      961 tcaggcggta atggttgatg ggcatcgacg cgatggtctc tcccgcgctc cagagcagaa
     1021 tcagccctgc gccgcccagg gcggtgaaaa tcggagcgcc gacggccacg gcgatcacca
     1081 gcgtcagcaa ggccggaatc cggagttgct cgggcgggat gggcgcccac gctcccattg
     1141 cgaccaccgc cgccgccagg gcgaacgcgc cgacgcgtcc gcgccacgtg gtgccggcat
     1201 ggcgtatcat ccgccacgcg acgagtgcga agccgatgca gagcaccaac tgcacgatcc
     1261 acaaagggac tccgtaggcc agggtctttc caagcctgcg gctggccaac acgaaatcca
     1321 tgcttgcctt gcaaagcagg aagctcatgg cggcggcaaa accgctgctg aagacgaggg
     1381 ccacctcctt gaacctccct ctgaaatggg cggacaaggt cgacaaggcc agcaatcggt
     1441 tgtcgcgcgc ggccaccgcg ccgccgagca tgcccagcac cagcaccagg tgctgcacaa
     1501 tcggcgccga cgcgggaatg cccgtgttga aaagcttgcg cagcacgatt tccgcgcacg
     1561 gcagcagaac catcgccgcc aggacgaata cgaggaccag gttctcgccc gtccgcacgc
     1621 cgcgcagcca gaccgggcgt tctggcccgg ccttgccgtt caggctgttg tccggggaga
     1681 actcgaggcg cacgggtggt tcactttcct tgattggcgc ggtattcgcg gatgaaacgc
     1741 aaggtctcgt cgaagacatc ggcgggcacc agcttgccgc gaatcttggg gtaaaccgct
     1801 tccgcttcag cccgccaggc cgcttcgatt tcgggcgtga cccgggtgac cttgagcccc
     1861 cgtttctcca tcgcggcaac ggattcggcg ctttcgcgtc gtccgttggc cttgatttcc
     1921 ttgccggcgg cggccgcggc ttcgagcagc tttgcgcggg tatcggccgg gattttctcc
     1981 caggcctggc ggcggacgat gcaggccccg atgagcgggg cccaattgag ttcgagcatg
     2041 tggggggcgc gtccgtcgat ctggctggcc agggcgaaaa ccggcggggc gggcgtggct
     2101 tcgatcaggc cggtttgcaa tccgggcacg atgtccgccg tctcgagcgg gacggggttg
     2161 aagccgccgg atttgtagat gacgacctgt tcggggttgc cggcccaagt gaagagcttg
     2221 agtttcttga ggtcctcggg atatgtgacc ggctccttcg agaagaaccg cacccagccg
     2281 gcatcggacc agaacagcac tacgaagccc ttttcgagca ggcgcttttc gagcatcggt
     2341 tcgagtttgc cgcaggcgta gtccacctcg gccaaatccc gaaagcccat cgggatgttc
     2401 tggaggccgg ccactccgaa ctcgacttcg gccagtccga cgccggtgag catagcggcc
     2461 tggatcgagt tgacactcat caggcccacg gtgtcggcct cgccccccag tttgccatcg
     2521 gcgtagatga ccaggtcgac gcccccattc gaggccttgc gccatgcgtc cttcatcgcc
     2581 aggaggctct tgtggaagct gcttccggtg ggcgccaggg tggcgagttt gatcttctcc
     2641 gctccgaggg tggcgccggc gcaggccagc cagaggctcc cggccaggcc gaggaaccag
     2701 cggctcatct gtcgtggtgc atgtcgattc atagtgcttg aatctgttga tcaggatgcg
     2761 gccgtcgcat cgcgcgcgct caaccgtccg atggttggtc caaaatcaga aacagttcgt
     2821 ctctcttgga catcagccac cgcgcccgac gctgcatgat cagattgacc aggcgcatct
     2881 ccggctgcgc atcgggatcg attgccagag cccgttcgag gaggatgtcg aactccttta
     2941 ggtcctgctt ctgcacgcag acagcctcgg cgaaggacac cagcggtcct gccaacagcc
     3001 cgcccgagag cgccagcgcc cgttcgaggt gctggcggga ccgttccgcc ggatcgccca
     3061 gcccgcccga tcgtgccatc tcgtaagcga tcaggaagct atgaatggcg cctttgtcga
     3121 aggcctcgtc caggaccagc gcacgatcga tgagcgcctc catttgggga atctcggcga
     3181 tcagacccgg atcatccttc gacatgctga tcgccgccgc ccacgatgtg gcggtccagt
     3241 acaggagcgg gacatccgcc ttgccgaccg cccgcacggc ctcgatgggc tgggcctgca
     3301 gtcccgcggc gaaaccgcga tgcgccgtct cgagcccgcg cagaccgtag tcgcgcgctc
     3361 gcagatagag gcgtctggca cgggcgcgca tcgcctgggc cttcgcaagg tcctggtcct
     3421 cggcctcatc ggcctcctgt tggacaaagg cgtaggcgta ctgggtgaag ccgctggcgg
     3481 tcgcgaagag caagccgcgg tggcgcgggc tttcggccag caggctctcc atcagtttca
     3541 ggctgaatgg gagcgccgct ccgacaagct cgggatcgtc gtcgctcgaa aacgtcgcgc
     3601 cgccgccggc cagagcgtcg cccaccctgt tgatggccat gcgtttgatc gagcaacccg
     3661 gcaggagaag cagcaggccc gccaaagcca cgcgcagcca gcgtccccac cggccgccag
     3721 ccttcgtttg cattgttgtc atgcggttca tctcaggacg attgatgttc cgactttgcg
     3781 ggctgtcgct caagcctgaa tgacattcgg gctttggcct gaccgcatgc cgcggctgcc
     3841 actgattgtg gcttcgtccc gtcggttctg ctcgggagga tcagtatcag gcggagcaga
     3901 gagtggccaa ttgataaaga cactgccgac attggcggat tcaatgcccc cggaatccaa
     3961 atcctcgtca acatatccgc gcggctcgac gcgcgcctcc tgtcctgtca ggatggcctc
     4021 cgtgaaacct gcaatcccgg acgatactga ggaatcagcg agatgcactc caccatcacc
     4081 ctcacccgtc ccgcatagcg ggacaccctc tcccacctgc ctgcgcccag gcttcggcag
     4141 gcaggtcgga tgggcgaggg ccggggtaag gggtctgtgt agggtatcca gccgactcgg
     4201 caccaagtcc ggcgcacgcc tcgcatgcca gagattctgc tcgccacact gaacgcgaag
     4261 cacatccact ccgcgttggg cctgcgctgc ctgatggcca atctcggccc cctgcgcgag
     4321 cgggcggcac tgcttgaatt cgacctggcc cagcgtccac tcgagatcgt cgagcaaatg
     4381 ctggccgtca gtcctaggat tgttggcctt ggggtctata tttggaacgt cgctccaatc
     4441 accgaggtgg ttgagctcct gagacgtctg cggcccggcg tggtgatcgt gctgggaggg
     4501 ccggaagtga tctgcgaaac cgatctgccg ctcgttgccg ggctggccga ctgggtcatc
     4561 gcgggcgaag cggacctcga attcgccgcg ttatgtcggg acatcctggc cgcaccgccg
     4621 cccgcgggag gagctgatgc gggcggccct ttcggggcag cggagcgccc ctgcccaagc
     4681 cccggaatgg cgcggaagcc gaggatcatc cgcgccgggc acgcccggct cgccgacctg
     4741 gccttgcctt atgagtttta tagcgacacc gacatcgcgc atcggatcgt gtatgtcgag
     4801 gcatcgcgcg gctgtccgtt cgcctgcgag ttttgtctct cgtcgctcga ggccttggtc
     4861 cggcagtttg atgtgaacct gtttctgggg gctatggaga ggcttctcga gagaggcgtg
     4921 cggcacttca agtttgtgga tcgcacgttc aacctgaaca tcgaggtctg cgtgcggatt
     4981 ctcgagtttt tcctggcgcg cctccgcgac gggctctttc tccatttcga gatggtgccc
     5041 gatcgtctgc cggtcgaact gcgcgaggtt ctggcccgtt ttcccgctgg gaccttgcag
     5101 atcgaactcg gaatccagac cttcaacgat gaagtcgccc accggatcaa ccgccgccag
     5161 gactgcgcgc ggatcgccga gaacctccgg ttcctgcgtc aacatactcg ggcgcatctg
     5221 cacacggacc tgatcatagg cctgcccggc gaacgcatcg agagcatcgc ggacggattc
     5281 gatcgattga tcgcgctggg gccgcacgaa atccagatga gcgtcctcaa gagacttcgc
     5341 ggagtccgca tcatccgaca cgacgatgcg tggggcatgg tctactcacc gctgccgcct
     5401 tacgagttga tcgagagcag gacgctcgat tttccgaccc tccagcggct ccggcgtttc
     5461 gcgcggtact gggacctgat cggcaacagc ggaaacttcg tggagaccac gctcctgctc
     5521 tgggccaagg gcggggaatc gccttttgcc ggcttcctgc gcctcagcga ttggctcttt
     5581 gcgcagacgg gccgccagca cgcaatcgct ttgccgcgtt tggcggagct tgctttcgaa
     5641 tacctcacgc gcgtgctggg atgcgacccg accgttgtgg cggccagcgt gctccgcgac
     5701 taccgccgtt cgggtcgcag cggggacctg gcgtttttgc gggaggacgt cgatcggaaa
     5761 accaaagccc atcgcgccgc gggcccgagg cgaagcgatc ggcgtcaggc tcgacatcgc
     5821 gcggcggacg atcaatccag acgcacggcc cggtagaagc gccgcggcaa ggacggagcg
     5881 ttggtgtcga cgagcgccac ggtgtcggtc gatggcgtct gcgagaggag cggttcccag
     5941 gagacgaggt cggtcgagac ttcgagccgc agcagtcgtc cgggcatggt ggcgatgcgc
     6001 atctgcagca tcccgccgtc gatcagcttc aaggcctcga agcggggcgg cgtcggcgga
     6061 ggcgtggtct gcacgttgat ggagacagtc gcgatcgggc tgtaggcgat cccatcccag
     6121 acccggtagg tgagactgtc gcttccggtg aagcccgacc ggggtgtgta gacgaagttg
     6181 gtgcccagtc catggacgcg tccgtgccgc gggccctcga ggatgacggc gcgcaggaca
     6241 ttggtgtccg aatcggtgac ttccagtaga aacgacaccg gggcatccag aggggctgtc
     6301 agccactgat tggtggtctc cggccgggtg ttggggtgga cgatcgacag ggcgaccacg
     6361 cccggcgcgc tctgcagttc gccgtcatgg accgtgaacg cgaatcggtc cggtccgtag
     6421 tagttggtgt ggggggtgaa gatgaggttg ggcgcggtgc cgctgaagcg tccgtgcgcc
     6481 gggttcgtga cgatggtgaa ctggagcgga tcgttttctc cgtcgatggc gcggagaacg
     6541 acattggtgg gcctgttcat cggcacggcg aggcggtacg attcggcgat cggtgccgta
     6601 ttctggtcgg tcatgcgcag cgagacgatg ccgggcgagc tggacgcctg gccgtcatgg
     6661 gcggtgaagg tgaagctgtc gtcgccgagg tagcccaagc gcggtgcata caaggcattc
     6721 gagccctcca cggtcaccgt gccgtgggcg ggctgccgcg cgatctcgaa cgacagcgga
     6781 tcggcatcgg catcggtcgc gtcgagcgtc agttcgatcg gacgcccgac tttggtggtg
     6841 agggactggg gcgcggcgat cggcgggttg ttgacggcca gcacgacgaa ccgaataacg
     6901 gcgggggaac tggcaagttc gccgtccctg gcgatatagg tgacggtgtc ttcgccggca
     6961 aatccgaagc gtgggtagta cgtggcgacg gtcggctggg cccaaaactc gccgtgatcg
     7021 gggcctttga cgatctcgaa gctcaacgcg tcaccgtccg gatcgaccgc ctggtagatc
     7081 acatcccgcg aggtattccg gttcaacacc agcacctggt cttgggccac gggcggatcg
     7141 ttcaccggct tgacttgcac cacgaccgtg ccgctcgttt catggccctg gccgtcggtg
     7201 atcgagtagc caaagctgtc gatcccggac cacccctgcc gggggcgata gaccagggac
     7261 gcgccggggc cctgctcgac cgcgccgttt gccggttggc cgaacccggt cagggcgagg
     7321 acatcgccgt cggcatcggt atcgttggcc agaacgtcga ttgcgatcgg tgtgttctcg
     7381 atggtaatga cccgatcctc gaccgcaacg ggcggatccg gcacagggcg cacccggagc
     7441 gtgacccagg cgagcgccat gccgtcctgg ccgtcatcga cgagatacgc gaaactgtct
     7501 tccccgaaga aatcgggttc gggttgatac cgcaacaggc cgttgccgag cgcctcgatc
     7561 gaccccgcgc gcgcccggct gaactggagg aggcgcaacg gcaatccgcg gggatgcgag
     7621 tcgttggcga ggggattcag tgtgcaggcg gtgtcttcgt ccaactcgac cacatcggcg
     7681 acggccagtg gcgggacgta gtccaggcgc acggcgaacg ttgccacggc gctgccgacg
     7741 agcccgcccg gatcgtgtgc cacgaaacgg agggtctcgg ctccggtcca ttcgggagtc
     7801 gggggttcga tcgcgacgac ccgattgtct tcgagtgcga cgagaaggtc ggatgccccc
     7861 tcgatcgtcc atgtcagcgc ggcgaccgga tgatccggat cgaggacgta gcggtcgagt
     7921 tcgatcggga agaacacacc gcccgcgcgg atggtctgac tcgggatgcc ctcgaccgcc
     7981 gggggctggt tggccggttt caagacggac acggagccgg tgtgggccgg atcgcgctga
     8041 aacatcggcc aaggcagatc gtccgggacg tgcggcgcgt cgagttccca ggcgtagatg
     8101 ctgtaccggc ccttggccac attcgccgcg gcccgcccgc cgtaaccata gtcctcgatc
     8161 gtgctggcca cgatgtcgag ccggccgttt ccgtcgaggt ccgtcagaac gggcggcccg
     8221 gccttgaagc ggttgcctgc gcccgtctcg gtgaccaggg cggtgacgtg cggatgcgga
     8281 tgcaggtcaa tcggtttgcc ggcgcgcgac catgccttca cgccgccgaa gtaggagatg
     8341 tctccagtcg ccgccgcctg gttcggatat cccggtgtga tcacgacgac gtccggccaa
     8401 ccgtcgccat tcacgtctcc cacaacgggc atcgacttga ccgagccgag catggggaac
     8461 agggacgcgt cgttggccag gattggtccg accggccaac cgggatgatc gaagccgtcg
     8521 tagcgcagga ggtggatcag cttctcctcc caattcaccg cgacaatgtc gagcgctccg
     8581 tcgtggtcca aatccccgag cgccggtgtg gccgtgaagg attcatggat caggaccggc
     8641 caaccggggc ggatttggcc ctcgcgatca taaacaaata ccccgcctcg ggtccccgtc
     8701 ttgccgcgag cggaggcatt gtgcgaggcc acaatcacct cggcccgatg atcgccgtcg
     8761 atgtcgccca caacgggaga gccgtacagg aatccgcccg tttgggcttg ccagacaggg
     8821 ccctccggct ggcgcagatc gaacatcgcc actccgcctt cgcccgcgat ggccacgatc
     8881 tccaagtcgg gatcgtcatc caagtcggcg accgccggaa actggcgcgg cacctcgagc
     8941 ggcagggccg cgtcagccat gaaccactgc tgccgaatgc gaccccaccg gtcgagcacg
     9001 gccagcatcc gccggccggt cggatgcgcg gctgagctgg ttccgtagac aacgagttcg
     9061 gccgtgccgt cgccatcgag atcggccagc accttgccga agttcccgcc cccggccagc
     9121 gccggccaat cgccatcgag cggacgcccg tcgtgccgga acgcccagat ccgaccgctg
     9181 tcgcccacgt ccacaaacac ctcgctcatc ccgtcgccat cgatgtcgcc aacggtggga
     9241 aggtctgtgg tgggaaaccc cgcggccaat gcccgcgacc acccgagggc accgtcgggt
     9301 cgatacacca gcagccgggc catcttcccc tcgaggttac ccggctcgac ggtcaggatt
     9361 tcctgcgcgc catcctgatc cagatcggcg acgacgattt gccgccagtc gttggtgctg
     9421 tagccggatg gcacgatcag gtgctgaggc cagccgggac ggagctgtcc gtccaggtgc
     9481 accagtgtgg cggtccagga gccgatggtt tcgccgcctg cccatgcggt cagcttgagc
     9541 gagtagaatg tgttgggggc cacagccccg gtgtcccacg ttcccaaaag actcccgatc
     9601 accggcgcct ggccgccatt cgccagcaca atcccatcgg tggaccatgc cgagggatcg
     9661 cgtccgatcc cgtgcgcgat ggtgtacgtg cggcctgtcc cgtagacagt gcccgtgagc
     9721 gggaggatgc tgccggcgcg gacgacgtcg gtgtgcagag gcgaggcgag gtggacgttc
     9781 cgaattgtca ccatcgcgcg atcgaccgcc tgctggtcca gagtgtccca gaccgtcaac
     9841 cgcaggacat aggagccttc gggaagactg tcggtcgacc aatccgccag cagggcgccg
     9901 cggtcgacgg gcagttcggc cgcgtggagg cgcgtccagt tggtggggta aacgccctgt
     9961 ccgtaatcga gggcatagcc ccggaaccgg gggctcgagg ccgtgccttc gagatcgatg
    10021 cggcccgaga ccaccggagg caattcgagt cgggctgtcg gcagcgggac gtcgacacgc
    10081 acggcccgcc atgcgttgag ccgcccggct ccgcagtaca cggcggtctg gatcggatcg
    10141 accgcgttgc gcaggatgtc ctcgacctgg acgggggtga actccgggtg gcgactgagg
    10201 acaagcgcgg ccacgccggc gacatggggc gcggccatcg atgtgccact ggcgacttcg
    10261 aaacggttgt cgggaacggt tgacaggatg ttctgtcccg gcgccgacag gtcgacatgg
    10321 gggccgtagc tcgagaagat ggagcgttcg tcgcgggaat ccagagccgc gacggtgagc
    10381 acttcgggat aggcggctgg gtacgacagc gcatcgctgc gggtgttgcc ggctgcggcc
    10441 acgatgacca tgccttgcgc gcgggcctgg gcgatggcct cggccagggc cttgctgttc
    10501 tcgaattgcc cccaactcgc gttgaggatg cgcgccccgt tgacaacggc gtagcgaatt
    10561 ccggcgatga cgtcgaaaag gctgccctcc ccgctcgcgt tgaatgcctt gatcgccatc
    10621 aggctggttt gccaacaaac ccccgtcacc ccgatctcgt tgttccccac cgcgccgatc
    10681 gtcccggcca cgtgcgtccc gtggatttgg tcgtccatgg gatcgctgtc gtcgctcacg
    10741 aaatcataac cgtgcacgtc atcgacgtat ccgttgccgt cgtcgtccaa gccgttgcca
    10801 gggatttcac cgggattcgt ccagacattc gcctcgaggt ccggatggta gaagtcgatg
    10861 cccgtgtcga tcacggccac ccggatggcg gcgtcaccgg ttgtcagggc ccaggcgaaa
    10921 ggagcccgta tgtcccctgc cgggcggccg gcggcggcat ccggattatg gaggttccag
    10981 agttcgtcaa agcggaaatc gttcggatag cgcgcaggat gcggatcgtc gaacagggtc
    11041 agaatcgtgt tcggctccgc gtaggccagc gcgggatgct gctgcaggcg ggcgagcacc
    11101 agatcggcgg attcgccggc cgcggctgtc accagggcga tccgatcgaa cacggatgtg
    11161 ccgggcgaag tccggccagc ccgggccgcg ccggaaccgg acagccatcg aatggacttg
    11221 atgcccgggc cgagatcaac gccggctgac gccggatgcg caaaggcggc tgcgtcgcga
    11281 aagcgcacca cgaattggtt gggcacaacc cgcgttgtcg gaaccgaagc ctcgaggtcg
    11341 gcgtggccct cgagccacgg gctggcgcct gcggcgggcg gcgcggcgtc cggcaaatgg
    11401 actgaatgga cgggcggctg tgcacccgcg tgtccgagca cccaaaccag gaccaggcca
    11461 agggcggccc atccccaggc tcgaaggagc catgaagacg cctttggtcg gttgcggacg
    11521 gtccgcggca tgacaggaca cccaaggagg tgcgcctcaa acccaatcca gttttcctcg
    11581 ccatgaggag tccccgtgtt ccctcgcccc cctctcgtgc gcctcgttcc ttagaatctt
    11641 tgcccccttc aacgcagcac ggaaactaga cttgtttact actggtttca ccagtcgaag
    11701 attctagtcc agaatcgcgg aaaccccaaa cacaaaacac attatgtcgg caggttgcgc
    11761 ttgcattcac gttcggacgg gacccatctt tggccgcccc gtatggacat gtggaacatg
    11821 ctgcttggct cgcttgccct gagcgtcgcg ccgatgggtc ttgcggctga tgggaaggcg
    11881 ctgccgaata tcctctgggt aactgggatg aatcgtcgaa ccgggcacat tggcggaacc
    11941 gcgcgcccgg ccagccgttc ttcgccctgt tcaacctcac cgccagccac gaaagccaaa
    12001 tccgcacctg cccgcatgcg ctgcgccacg acccgcccgg gcgcggctgc cggcatacca
    12061 tccggacacg cccgaggtgc gccatgactg ggctcagtat tacgacaaga tcagcgagat
    12121 ggacgaacag ttcggacgcc gcatcgagcc tcgatgccag tgcgacagag cggcttcgac
    12181 aaggcctgaa cgactgtggc agcggcgtcc gcgccttggc cgtgctgggc catctcatgc
    12241 atgtgccccg actgctcgac catagccctg cgcttcgttt ttttccgcca aagccatgaa
    12301 ctgtgccgac tggcaaggcg cgtccaatga gctgtccgat ccgggtccat ccgtgtctac
    12361 ccgtggttct gcgggcgggt tctggggact gaaggccggc gtggctttcc tgaatcacgg
    12421 ttcgtttggc gcgtgcccga agccgattct cgaactgcag gcagagctgc gccgccagat
    12481 ggaggcggag ccggtgcagt ttctctggcg ccattacgat gaacgaatcg aaccatcgcg
    12541 ccaggcgctc gcccgcttcg tcggcgcccg gccaagcgat ttggtcttcg tcaccaacgc
    12601 cacgacaggc gtgaacgctg tcgtacgctc gctgaatctc cgccggggcg acgcgctcct
    12661 gaccacctgc ctcgactaca acgcctgccg gaacgtgctt gtcgaggcgg cgcggcgcgc
    12721 cggcgcgcga ctgatcgtcg ttccagtgcc gttccccgtg tcgggcagcg acgccatcgt
    12781 ggcggccgtg ttgaaggctg tgacgccccg gacgcgtctg gccctgctcg atcacgtgac
    12841 gagcgactcg gccctcgtgt tgcctctcga acgcctcgtg cgcacgctcg aagatcgcgg
    12901 tgtgccgacg ctcgtcgacg gcgcccacgc gcccggcatg gttgcgctga acctcgcccg
    12961 cctccgcccc gcctactaca ccggcaacct ccataaatgg gtctgcgccc cgaaaggagc
    13021 ggccttcctc tgggcgcgcg aggaccttca gtccggtctc cagccgcctg tcgtcagcca
    13081 cggcaacaat cgatcccgac ccggtcatac ccgattccag gaccggttcg attggcccgg
    13141 gacaatcgat cccacgccct ggatgtgcgt cggggaagcg atccggtgga tggggagcct
    13201 cctgcccggc ggttggccgg cgctgcgggc ccgcaatcac gccctggcgg tgcaggcgcg
    13261 cgccttgttg tgccgaagtt ggagggtgga ttccccttgc cccgcgtcca tgctcggttc
    13321 catggcgaca gtgccgctgc ccgcgcgtct gcagaatcgc ccccccgctg gcaagatcga
    13381 tgcggatcag ctccgccttt acgacgagtt cggcatcgag gtcccgttca atcggatcgg
    13441 gcagccgccc atccgctatc tacgcgtctc ggcccagatc tacaacaccg tcgcgcagta
    13501 ccgccgactc aaccgggccg tccagatgct gtgaggaggc gttgcctgtt gcctggaaca
    13561 gttcgtccat cgacggcgag gtcgaggatc gccggacacc ggttggcaag gcgatccaga
    13621 ccaaaatcca ggagacaccg ccttcggcac caacaaccgt caccgaccag ggcctctccc
    13681 gcgaccgcag gattcccatc tcccatctcc gatctcctat ctcccagctc ccttctccca
    13741 tctccgatct cccatctccg aatccggcgc gtgcgatttg gatggaccga ttggggttga
    13801 atctgtcaaa tcgcatccga ttcgagaggg cgagagaagg cggcttttgt ggtaaaagcc
    13861 cttcgtctga tcgagtgaag gcggtgagcg ggtatgcacg acaaagaaat cacaacctac
    13921 ttggcgaacg tcctctatat cgccatccag gacggtcgac tgacatcgca ggaattggcg
    13981 gcgctcgatg aagtccggcg cttggttggc gccgaacgga ttcatgcgac cgaggccact
    14041 cgcctggtcg agaactcgaa gttcattccc caaggcgtgg gcactttcgg tgcccaggtg
    14101 cggaacctcg aggacatggt cttcgtgggg ttggcggatg ccgatctggt ggcgggcgag
    14161 cgcgaggcga tcgaggtgtt ctgtgaacag atcggcctca cccagaccca attggaccag
    14221 attgtccgcg aggcccaggt gcgttgttcg gatctcgaat tgcgcgctgc ccccggctcg
    14281 gatgcgaccg agagcaagag cgatgcgcac gacaacggca ggagcgcggg aaccgatggg
    14341 gcccagacct ggcacccaag gcagacgacc atcgcgcgcc gcgcatgcaa aacctcgatc
    14401 gaagactgcc gcgcggctgg gatcaagagc gtcgaattgc tgccatccaa ccgcgatggg
    14461 gagcagtgtg ctatggctct gagcatgcgc ggcacgccgc tggatatcga ccggcttccg
    14521 gagttgccgc ttccgtactg caccgcccgc cgctgccaat gcgtttggat tgcggcaggc
    14581 tagcccaacc tcatgccgga aagaggatac ggaccccgag ctctgtccgg tcactggatg
    14641 cgcaagaaac cacgcgagcg tcagcctgag tcccagcgcc gcgccatccc tgccgtcgag
    14701 caagtgctcc aggcactgag cgacgtcggc ctctcgagac cagtcgttgt cgcggtcgtg
    14761 cggcgcgagt tggggcggtg gcgcaagggt ggggctgtgc ctgctttcga gacggtcgtc
    14821 gacgggattc gaaacgcgct cgagactctg cggcgttcgg cactgcgtcc ggtgatcaat
    14881 gctaccggcg tagtcatcca caccaacctc gggcgggcgc ccttggggcc ggctgcgatc
    14941 gaggccttga cggccatcgg agccaactac agcaacctcg agatcgacct ggccagcggc
    15001 gagcgcggca gacgcgcggc gtacgtcgag caactcctgg ccgtgctttg cggggccgaa
    15061 gcggccaccg tggtcaacaa ttgcacggct gcgctcgtgt tgatgctgcg ccacttcacc
    15121 tcgggggcgc gcaaggaggt gatcctctcg agaggtgaac tggtccagat cgggggcgga
    15181 ttccgcatcc cggatgtgct cgagaccagc ggcgcgactc tgcgcgaggt gggaacgacc
    15241 aaccagacga ccctggcgga ttacgccgat gcgatcggtg agaagacggc gctggtcctc
    15301 aaggtgcacc gcagcaattt cttcatggag ggttttgtgg actcgccctc gatcgaggcg
    15361 ctggcggaac tggccaggcg caaacgcctg ccgctcatcg aggatctggg cagcggggcc
    15421 gttttgaaca cgaccagtta cggtgtcgat cgcgagccca cgccgcgcga atccctcagg
    15481 cgcggtgtcg gtctggtctg tttcagcgga gacaagcttc tgggagggcc gcaggcgggc
    15541 gtgatcgccg gcaaggcccg ctgggtcgcg gcgctcaagc gcgagccttt cttccgggcc
    15601 ttgcgctgcg acaagctcat cctggctgcg ctcgaagcaa cggctgaggc ctacgcgcgc
    15661 gcatctcgcg tcgggcgcgg gagcagcccg cccatagccc ctgccggcct gggtcgccaa
    15721 atccccgtgt ttggaatgct cgaaacaacc gaagagaccc tgcacgcccg ggctcatgcc
    15781 ctgcaaaccg aactgtccgg cctgcccctg tccatcacgg tcggggaggg caggggccgg
    15841 gtcggaggcg gcactctgcc gcgggccgca ctgccgtcgg tcacgctgga cctgtcgccc
    15901 cagacggtcg cgcccgccca gttcctggcg cgtctgcggt cgggctcgcc cccggtgatc
    15961 ggcttcctct ccggcggtcg cgtgcgcctt gacctgcgca ccgtgagtgc gggtcaggac
    16021 ggccttctgg ccgaagcgat ccgtatcgcg gcttgcggga tggcgccgga tcgagtcgaa
    16081 ccaagccggc gcacggcatg ctgaggaatc aacgagatgc acgacaccgt caccctcatc
    16141 cgccctccgg gcaccctctc ccatcggatg ggagagggcc ggcgtgatgg gtctgtgtcg
    16201 gctatccaaa cggctagaca gtgagtctcg aactggcatt tgcgcgaatg gcgcgctggg
    16261 acatattcca ccggctcgcc cctaggcgat gaccgtcgga cacgatgaaa accaaagcga
    16321 tcgcgaattg tctctgcgct tcgctggctg tgatggccgc cggcgtccag cctctctgcg
    16381 cacccgccgc cagtcccatg aacacgccaa ccgtcacagt gcgcctcctc ggcgcttcgg
    16441 gccaggttac cgaaccgctt gtcgttccca gggtggtcaa atccgatgcg gcctggcggg
    16501 cccaactcac agccgaacaa taccgcatca cccgcaccca cggcaccgaa cgggccttct
    16561 gcggcatctt ccacgacaac cataagtcgg gcctctatac gtgcatcggc tgcggcctgc
    16621 cactgttccg gtcggatgcc aagttcgatt ccgggacggg ctggcccagt ttcttccagc
    16681 cgttcgcgca agagaacatc ggccagacgc gcgacacgag tcacagcatg gttcgcatcg
    16741 aggtccactg cgcccggtgc gcttcccacc tgggccatgt gttcaccgac ggaccgccgc
    16801 cgacccggct gcgctactgc atcaactcgg acgcccttgc ctttcacgag cgtccgctcg
    16861 ccagccaggg gctggaacgt attgtcctcg gagcgaaccg catttctgga atcgaagcgg
    16921 ccttgcggcg cctggacggc gtctcggcag ttcggccacg caccacaggc ccctcgaagc
    16981 cactcggcca ggagaccctg tttgaggtcg agtacaatcc cgcgcagatt cccctcgcgc
    17041 gcctgatcgg cgtgttcttc gaaacgatca gtccggcgac tccaccgtcc ggtcagccgg
    17101 cgggagacgg gccggatcga gtcgcgttca acttccagac gccggagcag gaaacgatcg
    17161 tgcgcgcctc ggttgcaagg ctgctgcacg atcgccccga gtggaaagtc ggcgtcgata
    17221 tcagcctggc tcgttgagtc gggaacggca cggcgccgtt gacttggcct ggcattcgac
    17281 acaccctgcg cccatgaatc ctgtgattgc attggcggtt ctgtctttgg gcgcagcggc
    17341 ggttcaggcc gactggccgg agtttcgcgg gccctggggc aacggtcacg cggcgggttc
    17401 gccggatggg aagaccgtcg gtgtgccgtt gcactggagt gaaaccaaca acgtcagatg
    17461 gaagaccgca atcccccatc ggggctggtc gacccccgtt gtcatgggcg gccaggtctg
    17521 ggtgaccacg gcgaccgagg acggccatga ctttttcgtg ctcggcctgg atgcggacac
    17581 ggggaaggtc gtcgttcacg agaaggtgtt tcatagcgac aatccggagc cgctgggcaa
    17641 cggcgcctcg atgaactgct acgcgacccc ttcctcgctg atcgagccgg gccgagtcta
    17701 tttgcatttc gggagcttcg ggacggcgtg cctcgagacc gggaccggca aggtcctttg
    17761 gaaacgggac gatctgccct gccggcacta tcgggggccc tcttcgtccc tgatttcgtt
    17821 cgagaacctc ctgatcctca ccatggatgg cgtcgacgtg cagtatcacg tcgcgctcga
    17881 caagcagacc ggcaagacgg tctggaaaac cggccgatcg gtcgcctgga acgacgagca
    17941 tgtcccgggg caaatggcgc gcgacggcga cttgcgcaaa gcccacagca cgccgttgat
    18001 cgtcttggcg gccggccaga cgcaaatgct cagcgccggg gccaaggcgg cttatgcgta
    18061 cgacccgcgc gacggacgcg agttgtggag agtgcagtac aatgattggt cggcggcgcc
    18121 ccgccccctt tacgacaacg gattggcatt cttcgtcacc ggcttgtcaa agacggagtt
    18181 gctcgccgtg aagacggatg ggcaggggga tgtcaccgat accggcgtcg tgtggcggct
    18241 tagaacgcac gtcggcaaat attcttcgcc gattctcgtg gacgggctca tctacaccgc
    18301 cgcggaggac agctttgtca cctgtctcga agcggcgacc ggaaacaccg tctggaagga
    18361 acggatcggc ggcaagtacg cggcatcgcc ggtctacgcc gatggacggc tctatttctt
    18421 caatcagcag ggcacgacga ccgtcctcga accggggcgc gcgttccggg tcctggccac
    18481 caacaccctt gacgacggtt tcatggcctc gccggccgtg tccggccgcg ccttctacct
    18541 gcgcaccaag acccacctct accgcgtcga gtcgccgtag ccggaaagct cactctaccg
    18601 agcagttgac agggaaccgg acggttctca gcccgccaca caatcgtccg tggcctattc
    18661 agcccggcct ggttggcgtc tcgatagaag gggtcggagc tccgaggaat ccctcgtgga
    18721 ggaacaccgg gcgatagccg tgctcggtca cccagcggat ggcggcgtcg agccgttggg
    18781 ggtgatcggc gatgtaattc gcgtagaacg gccagaagta ctgttcgtgc gtgaacaggt
    18841 ccatgatctc cgcctggttg gggtctttcg cgagcggctc gagcgtgggc gcgatttggt
    18901 cgaggggcgt gccgttgcag acgatgtcca cgcgggagaa gacaatcccg ctgtcgaagt
    18961 gtttccagca atcgtgtcgg gagagatact ccgagaccgt gtcgtcgagc cggtagttca
    19021 cgtcatagcg tccgctccat ttgacgaagt agccgctgag cgcccgcaca ccgcgctcga
    19081 agagcggctt gagcgattcc tgccgcgtca tcccccagtg gatcacagtc ggaggggcgt
    19141 agctttgcgc cccggcgaat cggtgaatct gctcggccac ctgatcgaaa tcggcgatca
    19201 gtttctccgg ggacacatcc tcatacgggc gatccggctt atccgcgtgg gcgtggaaag
    19261 ccagcttgag ccagtccgca cagtcgcgcc actcgccttt gtagcggtcg gagagctgag
    19321 tcaggtcgaa gccatcggcc gttgtgtagt agatgttgac gctgaacttg accccgtagt
    19381 cccggtgcag cttgcggagc atcgccagat agaacgaatc gaaaagcgag cggtactgct
    19441 tctggtggat atcgcgcaga aagaagctgt tgtcatcgat cgagaaacgg taccggggaa
    19501 acgaatgccg gtcccagacg acacgcaccc gatcctcgag gcggcctgag ccgcccgcgc
    19561 aggtggccgt gatggtcgtc tcatactcgc gcaacacgac ctccgccgag aaggtctcgc
    19621 cctggcgttc ggcgtcccgg ccgcagacca tgacccgcgc gtccgcgggg gcgacgccgg
    19681 aaaccaggat tgccagtccg ccatccacag ccttgccatg gcgccggttc aagacggctc
    19741 catcgaaggg ctcgttgatt ctgaagggcg aggccccgga cgccaatgcg gtcaggcggt
    19801 cgccccatac gatccctgcc gtgcccagtg ccgccgatcc caggaagtca cggcgcctca
    19861 ttcgctgttg tttcatgctg tcgattcccc gccagcccgc gcagacttgg tgcctcggtc
    19921 tcagatgacc gttggacatt acccgggcct gcaggcacgt gtcgagcgga acgtccaggc
    19981 ggaagtcgtc cgtcgcgtcg caaagaacgt catggccgag aagaagaagg tgaaactgac
    20041 gcagagcggg ggcgccatgg cccaagagtc atgaacgcgt caccggccgg cgcttcgtca
    20101 atcagcctga gctgctccga caaaccgcaa tcggcggtga gggccttcac aggcaacaat
    20161 atgtgcagtg cagtgtcatc ggaacctcgg gcaggacttc cagttcatac tcgaaccagt
    20221 tgcccgtcgc gtagaccagg tcccgcggca cgtcgacctt gcccaattcg ccggccagga
    20281 ccagcgggcc gtacatcacc gccacaagat ccggatcgtc gggcattggg cacagccaga
    20341 gacacatcgg catctgcatc tccaccgtgg tctcgtcccg ccaggtgcgg cggatcgaca
    20401 ggaacgatcc gttctgcgcc gtcacggcct gatcctggcc gtcgatcttc agggtcacgc
    20461 cctccgtcgc ccattggggc acgcgcatgc gcagctcgaa ctcggtcggg gctgttgccc
    20521 ggatggtcag tttcgtcgct gaggtgtcgg ggaacgttgt gtcctggcgc acagtgacgc
    20581 cccgttcgcg ccaggtcagc tcgctggcga tgaattgatt cacccagagc gttcggtcgt
    20641 tgtgaaagca gatcgtgtcg ccgtacgtgg cgtggttctc gagaccggtc ccggtgcagc
    20701 accagaacga gtcgcggggc gtgttgaagt acttccaatg ccccgacgcc agag
//
LOCUS       DAOQRO010000008        20478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1793_length_20478_cov_5.556480, whole genome shotgun sequence.
ACCESSION   DAOQRO010000008 DAOQRO010000000
VERSION     DAOQRO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_20478_cov_5.556480"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKK20_00645"
     CDS             <1..244
                     /locus_tag="PKK20_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98429.1"
                     /translation="RLLVNLVNTSGPHRSEPIFDSITPVGPLDVVIREAAQPARVSLE
                     PGNRPLPFEHRNGQIRLTVPKVDIHDVILVELRPQR"
     gene            complement(683..2077)
                     /locus_tag="PKK20_00650"
     CDS             complement(683..2077)
                     /locus_tag="PKK20_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNU98430.1"
                     /translation="MMMHRVSKGRVRRWLACGLVLSMTSGLGAEPPGLEVGLAVREIT
                     PEVPIRLAGYAGRNRPADRVDHPLVVQAMALRNAADNRFVFVALDNCEVSRAFMEPVL
                     RELARRHEVPPGAVAVVSSHTHSAPVIEATLIDMAQPSADERQRIQQYSGRLRDTLVE
                     VVGAALADLQPAALEYGLGRATFAMNRRVYKGDRVVFGDNPDGPVDWDVPVLRVKGAD
                     GATRAIVFGYACHGTSVRSGDDWYVVSGEYMGYARQHLEELHPGTMAIYLTGMGADSD
                     PSPRGLLVHARRHGVELAGAILTVLNGPMRPVRGTMKTAYAEVELPLVDPPGRDRLEA
                     DAQSADVHIRQRAQAYLKRLDAGEPLPRAVPLPVSILRFGDDLTFIVMGGEVVVDYAR
                     QFKRLLANDNPWPVGYAYEVPCYIPTARLIKEGGYESDSSMIYYGFYGPFQTSVEDKL
                     VSCVTAMATDLRTP"
     gene            2236..2985
                     /locus_tag="PKK20_00655"
     CDS             2236..2985
                     /locus_tag="PKK20_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU98431.1"
                     /translation="MKKLQQAHCRAESRPSDHAFTLIELLVVIAIIAILAGMLLPALA
                     KAKEQGQRSRCIANVKQILAATHMYTTDYNDYMPYTSWSSDTFNVANWMYMRVATNRP
                     DHTVERGLLWPYHQAKNLYWCPLDKTNTTYFKLRDMKVGSYVMNGAVSGYSTGPRGAY
                     TTYRMAEFQPDCMLYWEPDEKQPSYFDNVASRPDEGVTQRHNTGTVMGMFGGQTEYMK
                     FKTYYIEAGIGGYPGIRPGRFWCNPGSRRGD"
     gene            3035..3427
                     /locus_tag="PKK20_00660"
     CDS             3035..3427
                     /locus_tag="PKK20_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98432.1"
                     /translation="MNLPSLPLLLAGLAGVTVLVLCGCGKGDSGAIEAAPPKEAASAL
                     EQAFDQAPAPVRDEVRQVSEAMRRGEYDKAVMSLQAVKEQSNVTLEQGMAVHGAMVSM
                     ESQLIQAIQSGDQNALRAYELLRALKRN"
     gene            3632..4837
                     /locus_tag="PKK20_00665"
     CDS             3632..4837
                     /locus_tag="PKK20_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98433.1"
                     /translation="MNQPSIVHAMPRRTFLATAAAAGTFAALKPSTALADSNSVLEIG
                     LIGCGGRGGWIADLFVQTGKYRLVACADYFQDRIDVVGDKFKIETARRYAGLSGYRRL
                     LESKLDAVVIETPPYFHPEQAAAAVDAGKHVFLAKPIAVDVPGCLSIGESGRRATAKK
                     LVFLVDFQTRANELYREALRRVRQGDMGRVVLVEAHYPWSSGGRDLPPASMEERLRRW
                     YHVKELSGDFIVEQAIHALDVATWIIDADPVRALGLGGRKLRPQGSIWDHFSVNYLFA
                     NEVPLSFTCIQTIPEMKDEIVARAYGSEGYIYTDYFGSVWIRGNEPFTGGSTGNLYTT
                     GAQTNIHEFHQFITQGKYDNPTVAPSVRSNLTAVLGREAGYQGGELTMAELMRENGKL
                     ELDFRGLKS"
     gene            4878..5282
                     /locus_tag="PKK20_00670"
     CDS             4878..5282
                     /locus_tag="PKK20_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNU98434.1"
                     /translation="MKLTRRSFVKGAMITSTGAAWALTSPEAGQAHAAPAATGSASDM
                     PRGKIGNLEINWCKSPTETAEFMKTVQKPWLAYKVMAAGAIPPADAFKYVLNNGADFV
                     LAGMFDFEIAENARVFRDTLAAIPERPRPWRA"
     gene            5304..6659
                     /locus_tag="PKK20_00675"
     CDS             5304..6659
                     /locus_tag="PKK20_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNU98435.1"
                     /translation="MLTRVHLWLLFALVACLSSPDHLARSMAAWRALVPTTPDFSPGP
                     DEQQVPRAMRSAMKDRPRLRVGVRDADIVGGDNRALQAAVDYVAGLGGGVVEIDAGEY
                     TMRDSLHLRPFVTVRGVRGKTVLRKAKAEVSPLALDGDYGEEQITVVDPDGFQIGDGV
                     AIWDSHSGGFHTTVARITGRNGNTFSISKPLNADCMIANRAQAATVFPLVSGYDLEGA
                     RLENLVIEGNRGANVPLNGCRGAGIFLYRGFGTVIENCVVRDYHGDGLSFQQSNDVIV
                     QDCIIEENAALGLHPGSGSQRPVVRRCIARHNGEDGLFLCWRVRHGLFENNLIENNGR
                     FGISIGHKDSDNLLRENQVRSNRLEGVFFREETLGMAAHRNRLERNLIENNGQDNEVA
                     GIRVRGQTRDLVFRHNTIRDTRPPEARKQTVGIRIEAGAGPVILEDNQIEADVKVQDR
                     R"
     gene            complement(6656..7978)
                     /locus_tag="PKK20_00680"
     CDS             complement(6656..7978)
                     /locus_tag="PKK20_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNU98436.1"
                     /translation="MSQWHTIEPQRAPSAATGIGGSVFAILAGLYIGLSLLKFGNPAI
                     LDHLIPVPGSALEVVFQSWPLRWSHIMLAGLVMVGLLTARWTLPRPRWLAFLPLVWLA
                     WQWIAATQTIDPRLTRVTLIHFHAVLAGFYLGLFALAPVRRIGLFWGGVAFGFALMVW
                     TGFEQRLGGLEATRQFVYSQPGWENLPAEHLKRLGSDRIFATLLYPNTLAGVLILLLP
                     PLLVVTWRRATWLSPAARGVIAGLFAVCGLACLYWSGSKAGWLIALVMLVVVLLRAPF
                     EKRWRAAIIIGLVSLGVAVFFVRFSGYFQKGATSVSARAEYWRAAWKTFADRPVFGSG
                     PGTFAVAYRQIKPPEAEMARLTHNDYLEQASDSGFVGLATYGTFVIGLALLLYRRCGA
                     DRMRFAVWLGVFGWVLQSGVEFGLYIPALGGTAFWLLGWLWGASEEGN"
     gene            complement(7975..9009)
                     /locus_tag="PKK20_00685"
     CDS             complement(7975..9009)
                     /locus_tag="PKK20_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98437.1"
                     /translation="MSFRQHTEQGHVVTLLQRSLERGRLGHAYLFSGSRLADLEAVAK
                     TLAKTLNCQSPPRQAANGLPLDCCDACTACRKIGGDIHADVQWLRPESKTRLVSIEQV
                     RELVHTMHLKPAEGLYKVAVVVAADRMTPSAANAFLKTLEEPPAKSVLMLLTTEPQRV
                     LETVISRCLRLSFAGDMGGPRQPEQLDWLRAFAGAAAARQQSLLGRYRLLGSLLAELA
                     RLKARISEQLTAQSPLQRYDDVDPDLRDRWETELAAATEAEYRRQRGELLACLQWWLR
                     DVWMQTQRIAGRSLAYPELETESGAVAGRVSTRDAMENLGVIERTQWLLGSNVQEALA
                     LEVGLLKLKL"
     gene            complement(9025..10488)
                     /locus_tag="PKK20_00690"
     CDS             complement(9025..10488)
                     /locus_tag="PKK20_00690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HNU98438.1"
                     /translation="MRKHYLACDLGAESGRLMLGTLDNGTLTLEELHRFKNGPVTSGN
                     SLHWDIPRLFGELETGLGIAASRGAPIASISADSWGVDYLLYDASGGIIEPTFHYRDA
                     RCPVGVMNVYAKVSWPEIFAETGLQFLQFNTIFQLAAESPERLERARMILGIGDGFNQ
                     WLSGVARFEESLASTTQLYDPRAKDWSRKLIARLGLPTGIFPPIVRSGTRLGPLRADL
                     ARRTGLTGIEVVASCSHDTGAAVAAVPGSGSDWAYLSSGTWSLMGVELETPVINDACR
                     ELNFTNEIGYGSSVRLLKNIIGLWLVQECRREWANAGADYDYAALTRLAAEAPAFVTL
                     INPADDRFLSPGDMPAKIAAFCREHRQPVPETPGAFVRCILESLALLYRRTLGLVDQL
                     TGRKTQRLHIVGGGSKNALLNQFTANALQIPVVAGPVEATAAGNILVQALAMGDLPSI
                     EAARQVVRDSFETATVHPCDASLWDEAYRRFAGLVGG"
     gene            complement(10642..11967)
                     /locus_tag="PKK20_00695"
     CDS             complement(10642..11967)
                     /locus_tag="PKK20_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HNU98439.1"
                     /translation="MNAIEKQVTRGPGGRILTNTGVWSPDGMWIVYDTRPDASGEVFE
                     GERIEMVNVGTGEVKVLYQARNGAHAGVATFHPIEPKVVFILGPENPTPDWTYGMSRR
                     RGVIVDVNRPGVAMNLDARDLSPPFTPGALRGGSHVHVWDAAGEWVSFTYEDQILSRY
                     AVETPDQEVNLRNVGVCAPGRAVRVSRLHPRNHDGAYFTVLVTRTVANPKPGSDDIKK
                     AFEEGWVGTNGYTRPDGSVQRRALAFQGHVVTADGQTISEVFLADLPEDLTAPGDGPL
                     AGTETRRPFPPKGVTQRRLTHTAGRKHPGVQGTRHWLRASPDGSRIAFLMKDDEGVVQ
                     LWTVSPNGGSPTQVTRNPWPVASSFTWSPDGRWIAHAMDRSVCVTEVRSGRTERLTEA
                     TTPDRAPRPEACVFSPDGSSIAYVRRIPSPVEPANQVFIVRLGNGVASK"
     gene            complement(12195..13580)
                     /locus_tag="PKK20_00700"
     CDS             complement(12195..13580)
                     /locus_tag="PKK20_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNU98440.1"
                     /translation="MSAAPSAQHTGSGPYVVWVCLVAALGGLLFGYDTAVIAGAIGFL
                     HEHFQLDATQKGWAAACALLGCVGGVAFAGVFSDRLGRKKTLVLAGVLFLVSAVGTAV
                     PKTFTTFVLYRVLGGVGVGIVSMASPMYIAEISPARIRGRMVSVNQFAIISGMLVVYF
                     VNYFIAQGAEPAWNAARGWRWMFASEIVPATLFLALLLFVPESPRWLAENGRTDQARR
                     VLARVVGPGAVDAELGSIETAAREKSASLAHLLKPGLRRMLGIGIALAVLQQVTGINV
                     FLYYAPEIFKQLGAGVDAALLQTIVVGAVNLLFTVVAIGRVDHWGRRPLMMAGAAGMG
                     VSLLALGLGAQRGLVSVSMLVFVLGYIACFALSVGPVTWVLLSELFPTRLRGRALSVA
                     TFWLWTANFVVSQTFPMLDENPWLVARFNHGFPFYVYAMFCVVLVLVVWRWVPETKGR
                     TLEEIERWSAG"
     gene            complement(13577..14839)
                     /locus_tag="PKK20_00705"
     CDS             complement(13577..14839)
                     /locus_tag="PKK20_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU98441.1"
                     /translation="MTSRERLQAAIEHRQPDRVPVDFGSTAVTGMHVSIVHRLRQRLL
                     GEPGYRVKVIEPYQMLGEIDDALREVLGIDVVGVPGRKSILGTQAKDWKPFTLFDGTP
                     CEVSGDFNVKPAPDGGWHIYPEGDTSAPPCGHMPEGGHFFDTIVRQDPIDEDRLDPAD
                     NCEEFAPLGADDLAYYRDRARWCGERGGLGTILSVPGTAFGDIALVPAPFLKHPRGIR
                     DVAEWYISTKTRRAYVTQVFERQCEVALANLATLTELLGDRIQIAFVTGTDFGTQRGP
                     FISVETYRNLFQPFHRQVNDFIHRRSAWKTFIHSCGSVYQLIPEFIEAGFDVLNPVQC
                     SAAEMGAERLKREFGRDLVLWGGGVDTQKTLAFGTPQDVYREVRERVDLFNRDGGFVF
                     NAVHNIQANTPIDNVMALFNALRDAGAA"
     gene            complement(14852..18109)
                     /locus_tag="PKK20_00710"
     CDS             complement(14852..18109)
                     /locus_tag="PKK20_00710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU98442.1"
                     /translation="MRKAKQPTSSRPAQTSRYDAQYSVLKDAYGAFADLRRELADSKA
                     AVAERTDLLDLFANCTDSQRAWLLLEDYFDKLSLARRDFAGNDWWPRLLGARGKARLE
                     QTALLFLRAGRPLPTELTSYANFGRLAEIEQEERERREIEALENWMLPSVPLHLDAPR
                     ARLRVLAKPVPHPGHAGLHDLECGFLLFRPRSGEKPRTLTELIELAKRAVHEQELFAP
                     HDWDFIAWLAETYRQGGRDGEPVRLNGVELLQWLAHWGNPPRIEWAGKPENVSFNGQL
                     AELTPHLANGESGSTLHQRLRLPTEEVLPLDKACFFAGRPALVAFHQTIYLLRNPPPP
                     SVLCALLASPGVSVRKLSHRLIAHLRKTASGSGAAWETLCVAHRARPQFLFDLSEDTI
                     RLRLVAVSERNNSRWQWNGHEWQRTDPPANLTGKPEMLDDPRLEAATAWLRRQDWFTP
                     EPGLWIGDANDNFFATLAQAWPDRPAEADYLGNASFQRLFLAPRQLRPRIVVQGSGID
                     WFAVSAAWEQEGLRLSAADLERLATAASRFVKLPDSGWVELDLSAVQKAHETMADIGI
                     DGLSALPQKVDLVQVTHMDEEGLQRFGDQPEAKALRGKLANFKGIPKVPLPGSVVAEM
                     RPYQKDGFDFLCHLGRIKLGGILADDMGLGKTLQTLAWLAWLRDQSRKPKPALVICPA
                     SVLHNWRREAEKFTPSLKVLVLESGPARHNLRKQIPQHDLIVTNYALLRRDLEALMKF
                     EFRAVVLDEAQFVKNPGAQITLSVKQLRAEHRLALTGTPLENRLLDLWSIVDFVQPNY
                     LGTQSQFHETYEPTGPDAEFTQRLARRKLSSKLRPLLLRRLKKQVAKDLPDRIEERRD
                     CELGDSQRKLYLAELRRSREQVMQTIQEKGLARSKMHVLAALTRLRQICCHPALVGSD
                     SPSGKTETLFELMEPLLEEGQKVLVFSQFVQMLQLIEAECKERQMPTYVLTGETKNRM
                     DIVGAFQADAKPSIFLLSLRAAGTGLNLTTASYVILYDPWWNPAVEAQAIDRSHRIGQ
                     TQTVNAYRLIAPGTVEEKIWELQQRKAQTIADVLGEEGFARSLTQTDLEYLFAED"
     gene            complement(18399..19529)
                     /locus_tag="PKK20_00715"
     CDS             complement(18399..19529)
                     /locus_tag="PKK20_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNU98443.1"
                     /translation="MKKKQSQTNDRYAIIMAGGRGERFWPVSREDRPKQLLALLGSRS
                     LLQDAVDRARPLVPTENILVITNRAQASAVRRQLPDLPRDNIIAEPCGRDTCAAVTLG
                     AALVGARSTTGVMAVLPADHVVSGAKSFEQVLRDGFDLAARGRVIVTIAIKPTEPATG
                     FGYIRVGAPLPPPQGAKTCRTTFHRAEQFVEKPPYERAVEYVNSGAYRWNAGMFIWSF
                     VTLVEGLSKHAPDMAEACHRWFKVAGTPKLAPTLAAEYPALRKISIDYALMEHAQNVV
                     VADGTFAWDDLGSWTALSHHLKADAAGNCAVGDFVHVDAARNIVFDARTKNRTVIAVV
                     GLRDSILVLTDDATLVAHKSQAQKVRELVARLAADEKRRALI"
     gene            complement(19526..20323)
                     /gene="trpA"
                     /locus_tag="PKK20_00720"
     CDS             complement(19526..20323)
                     /gene="trpA"
                     /locus_tag="PKK20_00720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNU98444.1"
                     /translation="MNRLVQRFGRLREEGRKGLIVYIGAGDPNLDTTRRLALAFDAAG
                     VDAIELGVPFSDPLADGLVNQLAAQRGLESGTTPGGVLATVKALRQGSHVPVVLYIYY
                     NLIHRYGQERFIRDAAEAGVDGLLVLDLPPEEAGGYGHQMRAAGLCAIYLIAPTTPEE
                     RVAGIAKEASGFIYYVSREGVTGMQATVAETIGDMTARIRRHTTLPIAVGFGISNPEQ
                     AKAVARHADAVVVGSAVVDRIARGAGRSDLVESVALFVRTLAEGIRG"
BASE COUNT         3778 a         6959 c         6304 g         3437 t
ORIGIN      
        1 caggctgttg gtgaacctgg tcaacacctc cggaccgcat cgaagtgagc cgatcttcga
       61 ttccatcacg cccgtcggcc cgctcgacgt ggtcatccgc gaggccgcgc agcccgcgcg
      121 ggtcagtctc gaaccgggca accgcccgct gccgttcgag caccgcaacg gacaaatccg
      181 gctcacggtt ccaaaagtgg acatccatga cgtgattctg gtcgaactcc ggccgcagcg
      241 gtaatgccat cccttctctc atgtgtgtgg gagaaggaag gggggagtgg gcgacaatgc
      301 gggctatgca gatgaatgtc gcggggtgag agcggtctgg ggaacctgaa tgctctggct
      361 ttgcggattc gcgttcctgg ccggcttcgt cgactcggta gtcggcggcg gtggcctgat
      421 ccagattgat tttctgaggg cctcggcgag cgcgaaggcg atcaacctgg ccacgaatct
      481 ttctgcggtc ggttattttg cggccaccga tcgcattctc tatgggtacg ctgtgccgat
      541 gggggcctgt aatcttctcg gttctattct gggcacgcgg atggcgatgt ggaggggcaa
      601 ccggtttgtg cgctggtttt tcctggtcgt ggtgggcgtt ctgatcctcc ggtacgggtg
      661 ggaggtcgct gcgccatccg gcctaagggg tccgcaagtc agtcgccatg gcggtgacgc
      721 acgagaccag cttgtcttcg acactcgttt ggaacgggcc gtaaaagccg tagtagatca
      781 tcgaggagtc gctctcgtat ccgccctcct tgatgagacg cgcggtcggg atgtagcacg
      841 gcacctcgta ggcgtagccg accggccatg gattatcgtt ggccaggagc cgcttgaact
      901 ggcgcgcata atccaccacg acttcgccgc ccatcacgat gaatgtcagg tcatcgccga
      961 atcggaggat cgacaccggc aggggcacgg cgcgcggcag cggctcgcct gcgtcgagcc
     1021 gcttcaagta cgcctgcgcg cgctgtcgga tgtgcacatc cgcgctctgg gcgtccgcct
     1081 cgagtctgtc ccttccgggc ggatcgacca ggggcagttc cacctccgcg taggctgtct
     1141 tcatcgttcc gcgcacaggg cgcatcgggc cattgagcac cgtcaggatc gcgcccgcca
     1201 gttcgacgcc atggcgcctg gcatggacca aaagacctcg tggcgacggg tctgaatcgg
     1261 cccccatgcc cgtcaggtaa atcgccattg ttcccggatg cagctcttcg agatgctggc
     1321 gggcgtagcc catgtattca cccgacacca cataccagtc gtcgccgctg cgaacgctcg
     1381 tgccgtggca ggcgtacccg aacacaatcg cccgtgtcgc tccatcggcg cccttgacgc
     1441 ggagcaccgg gacatcccag tccaccggcc cgtcgggatt gtcgccgaat accacgcgat
     1501 cgcctttgta cacccgccgg ttcatcgcga acgtggcgcg gcccaatccg tattcgagcg
     1561 cggctggctg gagatcggcg agggcggcgc cgaccacttc gaccagcgtg tcgcgcagac
     1621 gtccgctgta ctgttggatg cgctggcgtt catcggccga gggctgcgcc atgtcgatga
     1681 gcgttgcctc gatcacgggc gcggagtgcg tatggctcga aacgacggcg accgcgccgg
     1741 gcgggacttc atgccggcgc gccagctccc gcagcaccgg ctccatgaac gcccggctca
     1801 cctcgcaatt gtcgagcgcg acgaatacaa agcggttgtc cgccgcattt cgcaatgcca
     1861 ttgcctggac caccagcgga tgatccaccc tgtcggccgg ccggttgcgc ccggcataac
     1921 ccgcaagacg aatcggcacc tcaggcgtga tctcgcgcac cgccaatccg acctcgaggc
     1981 ccggcggttc ggcgcccaga cccgacgtca tcgacagaac caatccgcag gcgagccacc
     2041 ggcggacgcg ccccttcgag acacggtgca tcatcataag ccttggacct ttcgcttttt
     2101 gtatgcgaca cagacaacca tgtccctgcg cgaccggcaa acattttccc agacacccat
     2161 ggcgtttcgg ggccgggcga aaaactgcgt gacgggaggg ctttggtcgc tatatttggg
     2221 ggcaatcagc actttatgaa aaagctacag caggcgcatt gtcgcgctga aagtaggccg
     2281 tcggatcacg ccttcacgct gatcgagttg ctggtggtca tcgcgatcat cgcgatcctt
     2341 gccggcatgc tgctgccggc gctggccaag gccaaagaac aggggcagag gagccgctgc
     2401 atcgccaacg tcaaacaaat cctggcggcc acacacatgt acacgaccga ctacaacgac
     2461 tacatgccgt atacgtcgtg gagttccgac acatttaacg tggcgaactg gatgtacatg
     2521 cgggtcgcca ccaaccggcc ggaccacacg gtcgaacgcg gcctgctctg gccttaccac
     2581 caggccaaga acctctactg gtgcccgctg gacaagacca acacgaccta cttcaaactc
     2641 cgggacatga aggtcggcag ctacgtcatg aacggcgcgg tgtcgggtta ctcgaccggt
     2701 ccgcgcggcg cctacacgac ctaccggatg gcagagtttc aaccggactg catgctgtac
     2761 tgggaaccgg acgagaaaca gccctcctac ttcgacaacg tggccagcag acccgacgag
     2821 ggtgtcaccc aacgccacaa caccggcacg gtcatgggaa tgttcggcgg ccaaaccgaa
     2881 tacatgaaat tcaagacata ctatattgag gccggcatcg gcgggtatcc cggaatccga
     2941 cccgggcggt tctggtgcaa tcccggatcg cgcaggggcg actgaccgag gcggacatcg
     3001 ccccgaccgc ccccccacac aagagagtcg tcccatgaat cttccttctc ttcctctcct
     3061 cctcgcgggc ttggcgggtg tcacggtcct tgttctatgc ggctgcggaa agggagacag
     3121 cggcgcgatc gaggcggcac ctccgaagga ggccgcatcc gccctcgaac aggctttcga
     3181 tcaggccccc gcccccgtcc gggacgaggt gcgtcaggtt tcagaggcca tgcggcgcgg
     3241 cgagtacgac aaggccgtga tgtcgcttca ggccgtcaag gaacaatcga atgtcaccct
     3301 cgaacaagga atggctgtcc acggcgcaat ggtgtcgatg gaaagccaac tcatccaggc
     3361 catccagtcg ggcgaccaga acgccctgcg cgcctacgag ttgctccgcg ccttgaagcg
     3421 aaactagccc gcccccgaag cgcggcaccc ggcctgcaat cgctgcatgg tcaggtgcga
     3481 taggccgcgc gccctcacgc ggcgcacggg caaacatcgg tgcagacgcc gggtccggga
     3541 ccctgcaaag acgaggatgg atgttgtagg ccgcgtgctg tcgcgttgca tttggttgcg
     3601 cgaatcggct cgttcgttct agactccgcc catgaatcag ccttccatcg ttcacgcgat
     3661 gcctcgccgc actttccttg ccacggccgc ggccgccggc accttcgccg cgctcaaacc
     3721 gtccacggcc ctggccgact ccaacagcgt cctcgagatc ggtctcatcg gctgcggcgg
     3781 gcgcggcggt tggattgccg acctcttcgt gcaaaccggc aagtatcgac tggtggcctg
     3841 cgccgactat ttccaggacc gcatcgatgt cgtgggcgat aagttcaaaa tcgagactgc
     3901 ccgtcgctat gccggcttgt cgggctacag gcgcctgctc gagagcaagc tggatgcggt
     3961 cgtgatcgag acgcctccct atttccaccc cgagcaagcc gcggcggcgg tcgacgccgg
     4021 caagcatgtc tttctggcca aaccgatcgc cgtggatgtg cccgggtgcc tgagcattgg
     4081 cgagtcgggc aggcgtgcca cggcgaagaa gctggtgttc ctagtcgatt ttcaaacgcg
     4141 cgccaacgaa ctgtatcgcg aggccctgcg gcgggtgcgc cagggcgaca tggggcgggt
     4201 cgtcctggtc gaggcccatt acccgtggtc gtcgggcgga cgcgacctgc cgcccgcatc
     4261 gatggaggaa cggctgcggc gctggtatca cgtcaaggaa ctgtcgggcg atttcattgt
     4321 cgagcaggcc attcacgcgc tggatgtcgc cacgtggatc atcgacgccg acccggtgcg
     4381 ggcgctggga ttgggcggcc ggaaactgcg tccgcaaggg agcatctggg accatttctc
     4441 cgtgaattac ctcttcgcca acgaggtgcc gctctcgttc acgtgcatcc agaccatccc
     4501 ggaaatgaag gacgagatcg tcgcgcgggc ttacgggtcc gagggttaca tctacaccga
     4561 ttattttggc agtgtctgga ttcgggggaa cgagccgttc acggggggca gcacgggcaa
     4621 tctgtacaca accggcgccc agaccaatat ccacgagttt caccagttca tcacccaagg
     4681 caaatacgac aatcccaccg tggccccgtc cgtgcggtcc aacctcaccg ccgtgctcgg
     4741 ccgggaagcc ggctaccagg gaggcgaact gaccatggcc gaactgatgc gggagaacgg
     4801 gaagctcgaa ttggattttc gcgggctcaa gagctagcca ggcgcgcgat cagaccgatc
     4861 cacaaaagaa tcttgatatg aaactcaccc gtcgttcgtt cgtgaaagga gcgatgataa
     4921 cctcgaccgg cgccgcctgg gctctgacct ccccggaggc cggccaggcg cacgccgccc
     4981 ccgcggccac cggcagcgca agcgacatgc ctcgaggcaa gatcggcaac ctcgaaatca
     5041 actggtgcaa gagcccgacg gaaaccgcgg agttcatgaa gacggtgcag aaaccctggc
     5101 ttgcctacaa ggtcatggcc gcgggcgcca tccccccggc cgacgccttc aagtatgtct
     5161 tgaacaacgg cgctgatttc gtcctcgctg gcatgttcga tttcgagatc gccgagaacg
     5221 cccgagtttt ccgcgacacc ctggccgcga tcccggagcg cccccgcccc tggagggcct
     5281 agcgggagac acctttgcat cccatgctca cacgcgttca tctctggctc ctcttcgctc
     5341 tcgtggcgtg tttgtcgtcc cccgaccacc tcgcccggtc catggccgcc tggcgcgcct
     5401 tggttccgac gacgccggat ttctcgccag ggccggacga gcagcaagtc ccgcgcgcca
     5461 tgcgctcggc catgaaagat cggccgcgtc tgcgcgtcgg tgtgcgtgac gcagacatcg
     5521 tgggcggcga caaccgcgcg ttgcaggcgg ccgtcgatta cgtggccggc ctgggcggcg
     5581 gcgtggtcga gatcgacgca ggcgagtaca cgatgcgcga ttccctccat ctgcgtccat
     5641 tcgtcacggt ccgcggggtg aggggcaaga cagtgctgcg taaggccaag gccgaggtct
     5701 caccgctcgc tctcgatggc gattacggcg aggaacagat caccgtggtc gatccggatg
     5761 gcttccagat cggggacggc gtggccatct gggacagtca ttccggcgga ttccacacca
     5821 ccgtcgcgcg gatcacgggc cgcaacggca acacgttttc gatcagcaag cccctcaacg
     5881 ccgactgcat gatcgccaac cgcgcacagg cggccacggt gttccccctc gtcagcggct
     5941 atgatctcga aggggcgcgc ctcgagaatc tcgtgatcga gggcaacagg ggcgccaacg
     6001 tgcctctgaa cggatgccgc ggcgccggca tcttcctcta tcgcggcttt ggcacggtga
     6061 tcgagaattg cgtggtccgc gactaccacg gtgacggcct gagtttccag cagtccaacg
     6121 acgtcatcgt ccaggattgc attatcgaag aaaacgcggc cttggggttg cacccgggga
     6181 gcggctccca gcgccccgtg gtccgacgct gcatcgcccg ccacaacgga gaagacggcc
     6241 tgttcctgtg ctggcgtgtg cgccacgggc ttttcgagaa caacctcatc gaaaacaacg
     6301 gccgatttgg catctcgatt ggccacaagg acagcgacaa cctgctccgg gaaaaccagg
     6361 tgcggtccaa ccgcctggag ggcgtcttct tccgcgagga gaccctcggc atggccgccc
     6421 accggaatcg actcgaacga aacttgatcg agaacaacgg ccaggacaac gaggtcgccg
     6481 gcatccgcgt gcgcggccag acacgggacc tcgtcttccg ccacaacacg atccgcgaca
     6541 cgcgtccgcc tgaagcgcgc aaacaaaccg tgggcattcg aatcgaagcg ggcgccggcc
     6601 ccgtgattct ggaagacaac cagatcgagg cggatgtcaa ggtgcaggac cgacgctaat
     6661 tgccttcttc actggcgccc cagagccaac ccagcagcca gaatgcggtg ccgccgaggg
     6721 cagggatgta gaggccgaac tcgaccccgc tctgaagtac ccacccgaag actccaagcc
     6781 aaaccgcaaa tcgcatccgg tcggcgccgc accgccggta aagcaggaga gccagcccaa
     6841 tcacaaacgt gccgtaggtc gccagcccca caaagcccga gtcggacgcc tgctcgagat
     6901 aatcgttgtg ggtcagtcgg gccatctccg cttccggcgg tttaatttgg cgataggcca
     6961 ctgcaaacgt ccccggcccc gagccgaaga cggggcgatc ggcaaaggtc ttccaggccg
     7021 cccgccaata ctccgcgcgg gcgctgacgc tggtggcgcc tttctggaaa taaccggaaa
     7081 accggacgaa aaagaccgca actccaaggc tcaccaggcc aatgatgatg gctgcccgcc
     7141 atcgcttctc gaacggcgcg cgcagcagca ccaccaccag catgaccagc gcgatcaacc
     7201 agcccgcctt cgatcccgac cagtaaagac aagccaatcc gcacacggcg aagagtccgg
     7261 cgatcacgcc ccgggccgcc ggactgagcc aggtcgcccg ccgccatgtc accaccagca
     7321 aaggcggcag cagcaggatc agaaccccgg ccaacgtgtt cgggtataac agggtggcga
     7381 agatgcggtc gcttcccaat cgtttgaggt gctccgcggg caggttctcc cagccgggtt
     7441 gcgaataaac gaattgccgc gtggcttcga gaccgccaag ccgctgctcg aatccggtcc
     7501 acaccatcag cgcaaaaccg aacgccacgc ccccccagaa caatccgatg cgccggacgg
     7561 gggcgagggc gaacaggccc agatagaaac cggcgagaac ggcgtgaaag tggatcagcg
     7621 tgacgcgcgt cagccggggg tcgattgtct gcgtcgccgc gatccactgc cacgccagcc
     7681 aaaccaaagg gagaaatgcc agccaccgcg gacggggcag ggtccatcgt gccgtaagga
     7741 gcccgaccat gaccaggccc gccagcatga tatgggacca gcgcagcggc caggactgga
     7801 acacgacttc gagggcgctg cccggcacgg ggatgaggtg gtcgaggatg gccgggttcc
     7861 cgaacttgag caggctcaac ccgatgtaga gcccggccag gatcgcgaaa acgctgcctc
     7921 cgatcccggt tgccgcggac ggagcgcgct gcggctcgat cgtgtgccac tggctcacag
     7981 cttcaacttc agcagcccaa cctcgagggc gagtgcctcc tggacattcg agccgagcaa
     8041 ccactgcgtc cgctcgatga ccccaaggtt ctccatcgcg tcccgggtcg agacacgccc
     8101 agccaccgcg ccggactcgg tttcgagttc ggggtaggcg agcgagcggc cggcgatccg
     8161 ctgcgtctgc atccagacgt cccgcagcca ccattgcaga caggcgagga gttctccgcg
     8221 ctgccggcga tactcggctt cggtggccgc cgccagctcg gtctcccatc ggtcgcgcag
     8281 gtccggatcg acatcgtcgt accgttggag cggagattgg gccgtcaatt gctcggatat
     8341 ccgggctttc aaacgggcca gctccgccag caacgatccc aacaaccggt accgccccag
     8401 caggctttgc tggcgcgcgg cagccgcgcc ggcgaaggcc ctcagccaat cgagctgctc
     8461 gggctggcgc ggcccaccca tgtcgcccgc gaagctcagc cgcaggcagc gcgaaatcac
     8521 tgtctcgagc acccgctgcg gctcggtcgt caggagcatc agcaccgact tcgcgggcgg
     8581 ctcttcgagc gtcttgagga acgcgttggc cgcgctgggg gtcatgcggt ccgcggccac
     8641 gaccacggcg actttgtaga ggccttcggc cggcttgaga tgcatcgtgt gcaccagctc
     8701 gcggacctgc tcgatgctga ccagacgcgt cttggattcg ggacgcagcc attggacgtc
     8761 ggcgtgaatg tccccgccga tcttgcggca ggccgtgcag gcgtcgcagc aatccaacgg
     8821 aagcccattg gctgcctggc ggggcggaga ctggcagttg agggtcttgg ccagcgtctt
     8881 cgccaccgcc tccaaatccg caaggcggct gcccgaaaaa agataggcgt gtcccaaccg
     8941 cccgcgttcg aggctccgct gaagcagcgt caccacatgc ccctgctcgg tgtgctgacg
     9001 gaaactcatg acctcatccg tcctttaccc cccgaccagc ccggcgaacc gccgatacgc
     9061 ctcgtcccaa agagaggcat cgcatgggtg gaccgtcgcg gtctcgaacg aatcccgcac
     9121 cacttgccgc gccgcctcga tcgatggcaa gtcgcccatc gccagcgcct gcaccagaat
     9181 gttgcctgcc gccgtcgcct cgaccgggcc agccaccacc ggaatctgca gggcgttggc
     9241 cgtgaactga ttcaaaaggg cgttcttgct cccgccccca acgatgtgaa gccgttgcgt
     9301 cttgcgcccg gtcaattggt cgaccaggcc cagggtgcgg cgatagagca acgccaggct
     9361 ctcgaggatg cagcgcacaa acgcgcccgg cgtttctggg accggctgcc ggtgctcccg
     9421 acaaaaggcc gcgatcttcg ccggcatgtc gcctgggctc aggaatcggt cgtccgctgg
     9481 attgatgagc gtcacaaaag ccggcgcctc ggcagccagg cgcgtcaggg cggcataatc
     9541 gtagtccgcg cccgcgttcg cccactcgcg gcggcattcc tgcaccagcc acaggccgat
     9601 gatgttcttg aggaggcgca cggagctccc gtagccgatc tcgttcgtga agttcaactc
     9661 gcggcacgcg tcgttgatca cgggtgtctc gagttcgact cccatcagcg accacgtgcc
     9721 ggaactcaga tacgcccaat ccgagcccga gcccggcacc gccgccaccg ccgcccccgt
     9781 gtcgtgtgag cacgaagcga ccacctcgat ccccgtcagc ccggtccggc gcgccaaatc
     9841 cgcccgcaaa ggccccagac gcgtccccga gcggacgatg ggcgggaaga tgcccgtcgg
     9901 caatccaagc cgcgcgatca gcttgcgaga ccaatccttc gcgcgcgggt cgtaaagctg
     9961 cgtcgtgctg gcgagcgatt cctcgaaccg ggccacgccc gagagccact ggttgaaccc
    10021 gtcgccaatg cccagaatca tccgcgcccg ctcgaggcgc tcaggcgatt ccgccgcaag
    10081 ctgaaaaatc gtgttgaatt gcaggaactg cagtccggtc tcggcgaaga tttccggcca
    10141 gctcaccttg gcgtagacgt tcatcacccc gaccggacac cgcgcatccc ggtaatgaaa
    10201 cgtcggctcg atgatcccgc ccgacgcgtc gtagagaaga tagtccacac cccacgagtc
    10261 ggcgctgatg ctggcgatgg gcgcgccgcg gctcgcggcg atcccgagcc cggtctcgag
    10321 ttcgccgaac aggcggggga tgtcccaatg cagggagttg ccggacgtga ccgggccgtt
    10381 cttgaatcga tgcagttcct cgagcgtcag cgtcccgttg tccagcgtgc ccagcatcag
    10441 gcgcccgctc tcggcaccca gatcgcaggc aaggtaatgt tttctcatgc ggctcaagac
    10501 ctgcatttga tcctcggctt cccttccctt caagccggaa ctctcgtccg attcccttgc
    10561 cgcacggttg tgccacggcc cagacaatcc gatcacgcgc ggcgttgtgc cgctctccgt
    10621 cactgaggac gcaattgtca actatttaga tgcgacccca ttgcccagtc ggacgataaa
    10681 gacctggttg gccggctcga cgggcgacgg gatgcggcgg acatacgcga tgctcgatcc
    10741 gtcgggcgaa aacacgcacg cctccggacg cggggcccgg tcgggagtcg tggcctcggt
    10801 caatcgctcg gtccgaccgc tccgcacttc ggtcacgcag acactgcgat ccatcgcatg
    10861 tgcgatccat cgtccgtccg ggctccacgt gaaactcgaa gcgacgggcc atggattgcg
    10921 cgtgacctgg gtcggcgaac ccccgtttgg cgacacggtc cacagttgaa ccacgccctc
    10981 atcgtccttc atgagaaacg caatccggct gccgtcgggc gacgccctca gccagtgacg
    11041 cgtgccctgc acccccggat gcttgcgccc cgcggtgtgg gtcagccgcc gctgcgtcac
    11101 gcctttgggc ggaaacgggc ggcgcgtctc ggtcccggcc agcggaccgt cccccggcgc
    11161 cgtgaggtct tcaggcaaat ccgccagaaa cacctcgctg atggtctggc cgtcagccgt
    11221 caccacgtgt ccctggaaag ccagggcccg gcgctggacc gatccgtccg gccgcgtgta
    11281 tccgttcgtg cccacccagc cttcctcgaa cgccttcttg atgtcgtcgg agcccggctt
    11341 cgggttcgcg accgtccggg tgacgagcac cgtgaagtac gcgccatcgt ggttgcgcgg
    11401 atgcagtctc gaaacgcgca ccgcccggcc aggcgcgcag acgccgacat tgcgcaagtt
    11461 cacctcctgg tccggcgttt cgacggcgta ccggctcaga atctggtctt cgtaggtgaa
    11521 gctcacccac tcgcccgccg cgtcccacac gtgcacgtgc gaaccgcccc gcaacgcgcc
    11581 gggcgtgaag ggaggtgaca ggtcgcgcgc atccaggttc atcgccacgc cgggacggtt
    11641 cacatccacg atcacccccc ggcgccgcga catcccgtag gtccaatcgg gtgtggggtt
    11701 ctcaggcccc agaatgaaca cgaccttcgg ctcgatcgga tggaacgtgg ccacgccggc
    11761 atgcgccccg ttccgggcct ggtaaagcac cttcacctcg cccgtgccga cgttgaccat
    11821 ctcgatccgc tcgccttcga acacctcgcc cgacgcgtcc ggacgcgtgt cgtagacaat
    11881 ccacatgccg tcgggcgacc agacgcccgt gttcgtcaga atgcggccgc cgggaccgcg
    11941 cgtgacctgt ttctcgattg cgttcatggc gattctcgac gaaggcatcg gttcgggctc
    12001 ctcggattcc gtcgacggat ggcctcccgc cccggacacg gcgatcgcac accaacaagc
    12061 cgccgcggtt gcggcccacc gaagcctcgg ccgcgccggc gggacggcgg accgtccgcc
    12121 agctcttggg tccgtgcaaa aatcaattcc ggaagtaatt tttgcacaga cccttgtgtc
    12181 atggaaacgc atcatcagcc ggccgaccac cgttcgattt cctcgagtgt tcgccccttg
    12241 gtctcgggca cccatctcca aaccaccagg accaacacga cacagaacat ggcgtacacg
    12301 tagaacggaa agccgtggtt gaaccgcgcc accaaccagg gattctcgtc cagcatcggg
    12361 aaggtctgcg acaccacgaa atttgccgtc cagagccaga acgtggcaac cgacaacgcc
    12421 cgcccgcgca gccgggtggg gaagagttcg gacaacaaca cccaggtcac gggtccgacg
    12481 gaaagcgcga agcaggcgat gtacccgagc acaaagacga gcatcgagac cgagaccagg
    12541 ccgcgttgcg cacccaagcc gagcgccagc agcgagacgc ccatgccggc ggccccggcc
    12601 atcatcagcg gtctgcgtcc ccagtgatcc accctgccga tggccaccac tgtgaagagg
    12661 agattcaccg cgccgacgac aattgtctgc aacaaggcgg catcgacacc ggcccccaat
    12721 tgcttgaata tctccggtgc gtaatagagg aacacgttga tcccggtcac ctgctgcagc
    12781 acggccagcg caatgccgat cccgagcatc cgccgaagcc ccggcttgag caagtgcgcc
    12841 aacgaggccg acttctcacg cgccgccgtc tcgatcgagc cgagttccgc atcgaccgcc
    12901 cccggaccga ccacccgcgc cagcacacgc cgcgcctggt cggttcgccc gttttccgca
    12961 agccaccgcg ggctttccgg aacgaacaac agcagcgcga gaaagagcgt cgcgggcacg
    13021 atttcggacg cgaacatcca acgccagccg cgggcggcat tccacgcggg ctcggcgccc
    13081 tgcgcgatga agtagttcac gaagtagacg accagcatcc cgctgatgat ggcaaactgg
    13141 ttcaccgaca ccattcgtcc gcgaatgcgc gcgggcgaaa tttcggcgat gtacatcggc
    13201 gacgccatcg agacgatgcc cacgcccacg ccccccagca cgcgatacag gacgaacgtc
    13261 gtgaacgtct tgggcacggc cgtccccacg gccgacacga gaaacagaac gcccgccagc
    13321 accagcgtct tcttccgccc cagtcggtcg ctgaagacgc cggcaaacgc gaccccgccc
    13381 acgcaaccga ggagcgcgca ggcggccgcc cagccctttt gcgtggcatc gagctggaaa
    13441 tgctcatgca agaacccgat tgcgcctgca atgacggcgg tgtcgtagcc gaacagcaac
    13501 ccgcccaacg ccgcaaccag gcagacccaa accacgtagg gcccgctgcc tgtgtgctgg
    13561 gccgacggcg cggcgctcat gcagcgcctg cgtcgcgcaa ggcgttgaac agggccatca
    13621 cgttgtcgat cggcgtgttg gcctgaatgt tatggacggc attgaacacg aaaccgccgt
    13681 cccgattgaa aagatcaact cgctcgcgca cctcgcggta gacgtcctgc ggggtgccga
    13741 acgccaatgt cttctgcgtg tccacacccc cgccccacaa gacaaggtca cgtccaaact
    13801 cccgcttcag tctctccgcc cccatctccg ccgcggagca ttgcaccgga ttgaggacat
    13861 cgaagcccgc ctcgatgaac tccgggatca actggtaaac cgagccgcag gagtggatga
    13921 aggtcttcca ggccgagcgg cgatggatga aatcgttgac ttgccgatga aacggttgga
    13981 acagattccg gtacgtctcg accgagatga acggaccgcg ctgtgtgcca aaatccgttc
    14041 cggtgacgaa cgcaatctgg atgcggtcgc ccaagagctc agtcagcgtg gccagattcg
    14101 ctaaagccac ttcgcattgc cgctcgaaca cctgcgtcac gtaggcccgg cgggtcttgg
    14161 tcgagatgta ccactccgcg acgtcccgaa tgccgcgcgg gtgcttgagg aagggcgccg
    14221 gcacgagcgc aatgtcgccg aatgccgtcc ccggaacgct caggatcgtc cccaagccgc
    14281 cccgctcgcc gcaccaccgc gccctgtccc ggtagtacgc aagatcgtcc gcccccaacg
    14341 gcgcgaactc ctcgcagttg tcggccgggt cgagccggtc ttcatcgatg gggtcctgcc
    14401 gcacgatggt gtcgaagaaa tgccctccct cgggcatgtg gccgcagggg ggcgcgctcg
    14461 tgtcgccttc cggatagatg tgccagccgc cgtcgggcgc tggcttgaca ttgaagtcgc
    14521 ccgagacctc gcacggcgtg ccgtcgaaca gggtgaatgg tttccagtcc ttggcctggg
    14581 tgccgagaat cgacttgcgc cccggcacgc ccacaacgtc gatacccagg acctcccgaa
    14641 gcgcatcgtc gatctccccg agcatctgat acggctcgat gaccttgacg cggtagccgg
    14701 gctcgcccag aagacgctgg cgcaaccggt ggacaatcga gacgtgcatt ccggtcaccg
    14761 ccgtcgagcc gaaatcgacc ggcactcggt cgggctggcg atgctcgatc gcagcctgca
    14821 aacgctctct gcttgtcata gcgatgaata tctagtcttc cgcgaacaga tactcgaggt
    14881 cggtctgggt cagactgcgg gcgaatccct cttcgcccag gacatcggcg atcgtctggg
    14941 ccttgcgctg ctgcaattcc cagattttct cctcgaccgt gccgggggcg atcaggcgat
    15001 aggcattgac ggtctgcgtt tggccgatgc gatgcgaccg atcgattgcc tgggcctcga
    15061 cggccgggtt ccaccacgga tcgtacagga tgacataact ggccgtcgtc aggttcagac
    15121 ccgtcccggc cgcgcgcaga ctcaggagga agatcgacgg cttggcgtcg gcctggaacg
    15181 cgcccacgat gtccatgcgg ttcttcgtct cgccggtcag gacgtaggtg ggcatctggc
    15241 gctctttgca ctcggcctcg atcagttgga gcatctggac gaattgactg aacaccagga
    15301 ccttctggcc ttcttcgagc aacggctcca tcagctcgaa tagggtctcg gtcttgcccg
    15361 agggcgaatc gctgccgacc agggcgggat ggcagcagat ctgccgcaag cgggtcaggg
    15421 cggccaacac atgcatcttg ctgcgcgcga gccccttttc ctgaatcgtc tgcatcacct
    15481 gctcccggct ccggcgcaac tcggccagat acagcttgcg ctgcgagtcg cccaactcgc
    15541 agtcgcggcg ctcctcgatg cggtcgggaa gatccttggc cacttgcttc ttgagccggc
    15601 gcagcagcag gggacgcagc ttcgacgaca gtttgcgccg cgccagccgc tgcgtgaatt
    15661 cggcgtcggg acccgtcggc tcgtacgttt cgtggaactg ggactgtgtg cccaggtagt
    15721 tgggctggac gaaatcgacg atgctccaga ggtcgagcag ccgattctcg agtggggtcc
    15781 cggtgagggc aagccggtgc tcggcgcgca gttgcttgac cgagagcgtg atctgggcgc
    15841 cgggattctt gacgaactgc gcttcgtcga gaacaaccgc gcggaactcg aacttcatca
    15901 gcgcctcgag gtcgcgcctc aacagcgcgt agttggtcac aatgagatcg tgctgcggga
    15961 tttgcttgcg caggttgtgc cgggccggac cgctttccag aaccaacacc ttcagacttg
    16021 gcgtgaactt ctccgcctcg cgccgccagt tgtgcaggac cgatgccggg cagatcacca
    16081 gcgcgggttt cggcttgcgg ctctggtccc gaagccacgc cagccacgcc agggtctgga
    16141 gcgtcttgcc cagccccatg tcgtccgcca gtatcccgcc cagcttgatc cgcccgaggt
    16201 gacagagaaa atcgaagccg tccttctgat agggacgcat ctccgccacc accgagccgg
    16261 gaagcgggac tttgggaatc cctttgaagt tggccagttt cccccgaagc gccttggctt
    16321 cgggctggtc gccgaaacgc tgcaggccct cctcgtccat gtgggtcacc tgcaccagat
    16381 cgaccttctg cgggagcgcg gacaacccgt cgatgccgat atccgccatc gtttcatggg
    16441 ccttctgcac cgccgaaagg tccaactcga cccagccgct gtcgggcagc ttgacgaacc
    16501 ggcttgcggc cgtggcgagc cgctccagat ccgccgccga aagccgcagg ccttcctgct
    16561 cccacgccgc cgagacggca aaccagtcaa tgccgctccc ctgcacaacg atcctcggac
    16621 gcaattgccg gggcgccagg aacaggcgtt gaaaggaggc attgcccaga tagtcggcct
    16681 cagcggggcg gtcgggccag gcctgcgcaa gcgtcgcgaa aaaattgtcg tttgcgtctc
    16741 cgatccataa cccgggctcc ggagtgaacc aatcctgccg gcgaagccac gcggtggctg
    16801 cttcgagccg gggatcgtcg agcatctcag gctttccggt caggttggcc ggcggatccg
    16861 ttcgctgcca ttcgtggcca ttccattgcc atcggctatt gttccgctcg ctcacggcca
    16921 ccagccgaag ccggatcgtg tcttccgaga gatcgaagag gaactgcggc ctggcgcggt
    16981 gggccacgca gagcgtctcc caggccgcgc ccgaaccgct ggccgtcttc cgcaagtggg
    17041 cgatcaggcg atgactcaat ttcctgaccg acaccccggg gctagcgagc aaggcgcaaa
    17101 gcacggaggg cggcgggggg ttgcgaagca gataaatggt ctggtgaaag gccaccagtg
    17161 cgggccggcc cgcaaagaag caggccttgt ccaggggcaa cacttcctcc gtcggcaggc
    17221 gcaaccgctg gtgaagggtg gagccagact ccccgttggc caggtgcgga gtcagctccg
    17281 ccagttgccc attaaagctg acattctccg gttttccagc ccactcgatc cgcggagggt
    17341 tgccccagtg cgccagccac tgcagcaact cgacgccgtt gagccgcacc ggctccccat
    17401 cccgcccgcc ctgccggtac gtctcggcca gccacgcaat aaaatcccag tcgtgtggag
    17461 cgaacagctc ctgttcatgc acggcccgct tggccaactc gatcaactcg gtgagcgttc
    17521 gcggcttctc gccgctgcgg ggacggaaca agagaaaacc gcactcgagg tcgtgcaagc
    17581 cagcgtgacc cggatgcggg acgggtttgg cgagcacacg cagccgcgcc cgcggggcgt
    17641 cgagatgaag gggcaccgac ggcagcatcc agttctcgag ggcctcgatt tcccggcgtt
    17701 ccctctcctc ctgctcgatc tcggccaggc gcccgaaatt ggcatagctc gtcagttccg
    17761 tcgggagcgg acgtcccgcc cggaggaaga gaagcgcggt ctgttcgagc cgggccttgc
    17821 cgcgggcgcc cagcaggcgc ggccaccagt cgttgccggc aaaatcgcgc cgagccagcg
    17881 agagtttgtc gaagtaatcc tcgagcagaa gccaggcccg ttgcgagtcg gtgcagttgg
    17941 cgaacagatc gagcaagtcg gtgcgttcag ccacggcggc tttcgagtcc gccaattccc
    18001 gcctcagatc cgcgaatgct ccgtaggcgt ctttcagaac cgaatattgg gcgtcgtatc
    18061 tgcttgtctg cgccgggcgc gacgatgttg gctgcttggc tttgcgcatc agagtctcaa
    18121 tccatccatt ctcctataag atcaggtccg gtttgacaac acttgtttgc gcagaagccc
    18181 ctcggacccc gcgcaatcac ctcctcgcag acccgcgcgc ccgtgcgtgc ccacgcaacg
    18241 ccccccctat tcgccgaggc tcaaaccgat cggcggtccg gacattccac aacgccgcgc
    18301 gcgtgatcgg atgtccgggc tgtggcaaaa tcggacccgg aaagggaacc agtggcattc
    18361 taatggtctg tgcgccgacc gggtgtgtaa caggccggtt agatcaaggc gcgtcgcttc
    18421 tcgtccgcgg ccaggcgtgc aaccaattcc cggaccttct gggcctggct cttgtgcgcc
    18481 accagcgtgg cgtcgtccgt cagcacgaga atcgagtccc gcagccccac cacggcaatc
    18541 acagtccggt tcttcgtgcg ggcgtcgaac acgatgttgc gcgcggcgtc cacatgcacg
    18601 aaatcgccga ccgcacagtt tcccgccgcg tcggctttga gatgatggct gagggcggtc
    18661 cacgaaccga ggtcgtccca ggcaaacgtg ccgtccgcca ccacgacatt ctgggcgtgt
    18721 tccatgaggg cgtaatcgat cgaaatcttc cgcagcgcgg ggtactcggc agccagggtc
    18781 ggggcgagct tgggcgttcc cgccaccttg aaccaccggt gacaggcctc ggccatgtcc
    18841 ggcgcgtgtt tgctcagccc ctcgaccaga gtgacaaacg accagatgaa catcccggcg
    18901 ttccaccggt acgccccgct gttgacgtat tccacggcgc gctcgtaggg aggcttctcg
    18961 acgaattgtt cggccctgtg aaaggtcgtg cggcaggtct tggcgccctg cggcggaggc
    19021 agtggcgccc cgacgcgaat gtacccgaaa ccggtggcag gctcggtcgg cttgatcgcg
    19081 atcgtcacaa tcacccgccc gcgcgcggcc agatcgaatc cgtcccgcag cacctgctcg
    19141 aaagacttcg cccccgacac cacgtggtcg gccggcagca ccgccatcac ccccgtcgtc
    19201 gaacgggccc ccaccagcgc cgcccccagc gtcaccgccg cgcatgtgtc gcgtccgcac
    19261 ggttcggcga tgatgttgtc ccgcggcagg tccgggagct ggcgacgcac ggcggacgcc
    19321 tgcgcgcggt tggtgatcac caggatgttc tcggtcggga ccagcggacg cgcgcggtcg
    19381 acggcgtcct gcaacagcga ccggctgccc agcagggcca gcagttgttt gggccgatcc
    19441 tcgcggctga ccggccagaa tcgctcgccg cgtcccccgg ccatgatgat cgcataccgg
    19501 tcattggtct ggctctgctt ctttttcatc cgcgaatccc ttccgccaga gtgcgcacaa
    19561 acaacgccac cgactccacc aggtcgctcc gccccgcccc gcgcgcaatc cggtccacca
    19621 ccgcgcttcc caccacaacg gcatcggcgt gccgggccac cgccttggcc tgctcgggat
    19681 tcgagatgcc aaagccgacg gcgatcggca gtgtcgtgtg ccggcgaatc cgcgccgtca
    19741 tgtcgccaat ggtctcggcg accgtggcct gcatgccggt gaccccttcg cgcgagacat
    19801 agtagatgaa cccgcttgcc tccttcgcaa tccccgccac gcgctcctca ggcgtcgtgg
    19861 gggcgatcag gtagatcgcg cacaggcccg ccgcccgcat ctggtgcccg tacccgccag
    19921 cctcttccgg cggcaggtcc agcacaagca aaccatccac gccggcctcc gccgcgtccc
    19981 ggatgaaccg ttcctgtccg tagcggtgaa tcaggttgta gtagatgtag agaaccaccg
    20041 gcacgtgcga cccctggcgc aacgccttca ccgtggccag cacaccgcca ggcgtcgtgc
    20101 cggactcgag cccccgctgc gcggccagtt ggttcaccag tccgtccgcc agcggatcac
    20161 tgaacggcac acccaactcg atcgcatcca cccccgccgc gtcgaacgcc agcgccagac
    20221 gccgcgtcgt gtccagattc ggatccccgg cgccgatgta gacaatcaga cccttccgac
    20281 cctcctcccg gaggcgaccg aaccgttgca cgaggcgatt caccctgccc agactgccaa
    20341 gaagccaacc aaacctcaag cggcgaaagc gagagcggtg aaagcgaaga gcggttctga
    20401 gctgggaggg ggtgattgag gaggagggac ttaacgaatg tgcggaacaa tcctccgccc
    20461 atctgttttg ctgggccg
//
LOCUS       DAOQRO010000009        20470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1798_length_20470_cov_5.991967, whole genome shotgun sequence.
ACCESSION   DAOQRO010000009 DAOQRO010000000
VERSION     DAOQRO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20470
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1798_length_20470_cov_5.991967"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1293)
                     /locus_tag="PKK20_00725"
     CDS             complement(250..1293)
                     /locus_tag="PKK20_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNU98445.1"
                     /translation="MNDALRLEPRGWQPATRRRLERLIRTGAGRHRSAAFDFDNTLVC
                     GDIGEATLALLERDGLIRAEHLPPHIAPEFRSRGGRRISPCSGADLTDYYEALLDPTA
                     HGGLDPTPLANGYVWAATVMQGLSVQDVARATREVWKLSRPGRNTFITTAPGRTGYPI
                     PFFYPEMIDLVAVLLQSGFDVWIVSASNVWSVRWMVLEALNPLLRSLRARIPIRADHV
                     IGVSTLLVDRHERFHKDSVLVRTDPAYADMNMRALARFRISDQIHFPAPVYSGKVAAL
                     WDVIGQPPFLAAGDSPGDLPMLTFATNRLWIARSNKPAYQAAMSAARQTSGAQNWLIQ
                     PTETAGQPGFKPE"
     gene            complement(1295..2602)
                     /locus_tag="PKK20_00730"
     CDS             complement(1295..2602)
                     /locus_tag="PKK20_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU98446.1"
                     /translation="MPPTSAPAASLWAWQLRIFWLLWGAYASYYLCRVNFAVAQPMML
                     KEFTDWTAAQIGTIPSVYAACYAAGQLVNGTLGQRYGARHLMTMALLTASFTNLLFSR
                     TSSFPVMLALWGVNGYAQSAGWSLLVQTIANWNTSERRGTVIGLISTCYQVGNVVSWL
                     LAGMLCDTVGWRAAFWVPSLILLPIAAAFFAFLRNAPEDAGFAPVRDDVGHDTPAEAS
                     RPACAATPAAALSTRQILALTLSNRVLWVLAVGFFCMNSVRYTFMNWSVQYMADFQGR
                     SIKGSALTAVAIPLIGSLGAIAAGWASDTLFGKRRAPVCAIMLIALAGVCVGFAWIPQ
                     GEWVWATIAMGLAGFFIYGPDMLMSGAATVDVSHPKAASIATGLTMCLGATGAIFSGA
                     GIGWLKDRAHGDWALVFHFIAGLAVVSALLMIAIWNARPKGAK"
     gene            3004..3729
                     /locus_tag="PKK20_00735"
     CDS             3004..3729
                     /locus_tag="PKK20_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNU98447.1"
                     /translation="MATLVFDIETAACSREEFDDAQWEFLHRDAERLADPAAREARRA
                     EIQHQFSLWPLTARVVCIAMLNADTARGQVLFTASDYDPGEGEGGAEPVEFVACIDES
                     EMLTAFWDVARHYDQIVTFNGRGFDVPFLYLRSALVNVPITRKEWLGYRFATEPHCDL
                     AEQLSFYGVSGRDGAARRFNLDFYCKAFGIDSPKSHGVTGMDVGRLTAEGRHREIAEY
                     CLRDVQATVHLYKIWRQRLAGIK"
     gene            3790..3930
                     /locus_tag="PKK20_00740"
     CDS             3790..3930
                     /locus_tag="PKK20_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98448.1"
                     /translation="MIRLVGWPDGAGNRHVLVAASHDEAAASPRSAILDSLVANPGAV
                     QS"
     gene            3958..4050
                     /locus_tag="PKK20_00745"
     CDS             3958..4050
                     /locus_tag="PKK20_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98449.1"
                     /translation="MATADIDGDGFGDLFLGQNFFGSGSDLTRD"
     gene            complement(4136..5539)
                     /gene="mpl"
                     /locus_tag="PKK20_00750"
     CDS             complement(4136..5539)
                     /gene="mpl"
                     /locus_tag="PKK20_00750"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNU98450.1"
                     /translation="MFRPEIRSFHFVGVCGTAMASVAGAMQDQGYVVTGSDENVYAPM
                     STFLAERGVEVMNGYAERNLAHRPDLVVIGNAISRGNPEAEAVLGHRMRYCSLPELLR
                     EYFIRGKRSLVVAGTHGKTTTTALLAWVFEHNGLNPSYLIGGIPNNFSQGARFTDSDW
                     FIIEGDEYDTAFFDKRSKFVHYLPEVAIINNLEFDHADIFRDLESIQTSFSHLIRLVP
                     RNGLVLANGDDPNLEPLLEFKHCPIKRFGFGEENHLRAFKLRLGATATEFETPNHKYH
                     LNLIGELNVRNALGVIACARHCGLTNAQIQAAFNTFKGVKRRLEVRGVAGGIVVIDDF
                     GHHPTAIRETLKALRLKHPAEKLWAVFEPRTNTTRRNVFQRELADAFLGADAVVIAQV
                     SRLELLAPHERLDPERLMADLQAAGKTAAYLPDVDSIVEHLAGSVQGGDVVCVFSNGG
                     FGGLHQKLLDRLAQRRA"
     gene            5641..6408
                     /locus_tag="PKK20_00755"
     CDS             5641..6408
                     /locus_tag="PKK20_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98451.1"
                     /translation="MNGPSGLSAPRPVPCWVRGPASLEMLSPRVQPPRLTALGGSVDT
                     PPEGITADLLIVRDFTELTNRAPEARAVGSLIGSPTPFSMQTPQTGMMRYEDGVPRIP
                     HAALSVEDLLLLQQELTRSKIHGRACRIRVTSGCRFLRKGRHDKLDATFSLSQLRGGS
                     AVRSAAGAVSPLSAGRRCASDRVGANGSELGWAAIDRCGEVLVPAVGHTRMHRAGGMG
                     VVHKARQPKLERLVALKLMQSSKRCATERMSGPNSGT"
     gene            6526..7239
                     /locus_tag="PKK20_00760"
     CDS             6526..7239
                     /locus_tag="PKK20_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNU98452.1"
                     /translation="MTTHDIINRRHFIETAGVAAAAAGFLGSAAPARAADAPPAANAK
                     VRIVGISGSHRKGKTTATGLQMALDSAKAVSPEIETELIELMDYKLLPAVPGGEIEDD
                     YARIHAKITDPSVGGIILGSPVYFSNMSALMKMFIEHFGPYKKSGALSNKVAGVLTVA
                     GVRNGGQEGVAQSIMAAALGVEMIVVGDGRPTGHSGATLWNNAKDDITQDEFGCSTAK
                     GLGKRVAEVALRMAGKARS"
     gene            7356..7994
                     /locus_tag="PKK20_00765"
     CDS             7356..7994
                     /locus_tag="PKK20_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98453.1"
                     /translation="MGERLRNRQEALGKAVSVGHVGVMRTAGAFHLRETTGTSGLEAA
                     LRSAVEALARHEVPHLVVGGLAVQEHGYFRVTTDCDLVVPDVLDAVEVLVADLSGSFV
                     KHGDCDDTVKYKRNGVLINLLPAGHVLKAGCRVPFPVPIEVWDEPRFVSLEKLIALKL
                     DSWSNSPNRRHKDKTDVIELIQALRLPRDLAVDESVRTLYVETWDALAAEPG"
     gene            complement(8061..8792)
                     /locus_tag="PKK20_00770"
     CDS             complement(8061..8792)
                     /locus_tag="PKK20_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98454.1"
                     /translation="MKAKTVLVAVLVAVAALLVWRMFRTPDRLARGRALDRREIALRV
                     LGEHLAGQTPGQAALVVANPFGQMPGQARAVYAFEEAAIRGLRSGWGNRIRLVGVDYP
                     ELTPAAIQNPSSVPLPPDTTTPLSFLTAQGAWDALALKHPDATLFVSVIGLPAGLQAL
                     DCWRNPTLRFALLLPDLRLIGDARAIAAAFKSGKIVAAVLNRPGAPPETAAIQRDDRD
                     EFEARYLFVTAANIDDLIEVFPTLF"
     gene            complement(8876..9532)
                     /locus_tag="PKK20_00775"
     CDS             complement(8876..9532)
                     /locus_tag="PKK20_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNU98455.1"
                     /translation="MRRRISTGFTLIELLVVIAIIGILASLLLPALGRAKARAKQVQC
                     VNNLKQIGLATLAYAQDNQGLVPINAPLDPGVTWASLLSTNENLRPLELFLCPSYPPP
                     KLTNWIRTYGVRLDPPTNYTRGAFSELLHVDSIANPLEYLHVADTTSRGRLGIGSQQF
                     YFFRAANENEVHARHSQRANGLFLDGHVEGCNRKRLESLGIAALFEVDTIPGYFGGGG
                     "
     gene            9672..11075
                     /locus_tag="PKK20_00780"
     CDS             9672..11075
                     /locus_tag="PKK20_00780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNU98456.1"
                     /translation="MLFNSGVFLQFFAAFLLLYYLVRNRLMARNGLIVLASYLFYGWW
                     DYRFLSLLVFSSLLDYAVGRALDRCTHPGHRKLWLALSLATSLGLLGFFKYYNFFIDS
                     AAVLLAQLGLSVEPRTLDIVLPVGISFYTFQTMSYTFDVYRRTMPATRDLLSFLAYVA
                     FFPQLVAGPIERASRLLPQFTKTRVITRQRLIEGTWLMIWGMFKKVVVADNLAPLVEM
                     VFDHPAPTGLMVILGTVAFTFQIYGDFSGYSDIARGLASVLGFDLMLNFNLPYTATSP
                     RDFWRRWHISLSTWLRDYLYIALGGNRRGVWRTRFNLLATMLLGGLWHGAAWSFVLWG
                     LWHGLGLVLTRPAQSHVPAPDRPRWRLLQWAATMLFVLYGWLLFRAGSLAHIVELTSA
                     LACWEWPTWGLSYLINLMAFSLPLALAEVWQARAGDLMTPARWLASMRALLQGILLLA
                     IVVFWQKEAAPFIYFQF"
     gene            11083..12192
                     /locus_tag="PKK20_00785"
     CDS             11083..12192
                     /locus_tag="PKK20_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98457.1"
                     /translation="MNPQDSIGGTSTKAMTTAQTHLRSHQCAFTARDLAEIVGAPLGV
                     LLLLAALFHFAAAADWLPAPRPTLDMDRTLLIHQADSSRSAQDATVILIGDSSCLMDV
                     SASDLTRHLGQPTLNLGLLSYLDLPSFGILVRNYAHANPERLETVVLLLHPESLRLQA
                     PSAYHRAQLEDYLAGRDAHTAGGIGGRLQTALGAILVRGRLEARVIPAPLSGVYGRFY
                     GFTRDFSRWLGAHRGSAIDPQRFDRTRAQGNAEYRLAPRLESESRLLRQCVPSGVRLV
                     VGLTPAPRSFVRPDHEATCARILEQLAKWLKADAVLAGLPASMPDELFASVTHLTGEG
                     QTLFTQRLAAEMQTAAIVRTPRPDWIPRAEHLMQE"
     gene            12213..13229
                     /locus_tag="PKK20_00790"
     CDS             12213..13229
                     /locus_tag="PKK20_00790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNU98458.1"
                     /translation="MVSDSNGDRRQLRMVPCPNCGVRLFLDGDLPPLSTVPCTKCGES
                     VMIPMRLRQFELQAVIGKGGMATVYRAFDTTLQRDVAVKLMNPELMQDQNGMNQFLRE
                     ARACGQLNHTNIIHIYTFDDLDGQAYLVMELADRGSLDSRIEKGPVSELDVLDIGIKV
                     TSALDTALKHGLLHCDIKPGNILFNAEGEPKLVDFGIARRTDAESDRDGTVLGTPYYI
                     APEKIRREQENFLSDMYSLAGTLYHAITQHVPFEAPTVEDVLAAHVHTPLTPPDRVNP
                     DITAPTSDALVRAMAKKPSDRFESYDAFIMALNAARSQYLVGRYINPQPARPGSRLKN
                     LFRR"
     gene            complement(13503..14693)
                     /locus_tag="PKK20_00795"
     CDS             complement(13503..14693)
                     /locus_tag="PKK20_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNU98459.1"
                     /translation="MTRVRTGLDVLVAEGFKRLRGQRVGLVAHPASVDARLRHAADLL
                     LAAPDVRLEVLFGPEHGWMGHAQDLEAVPSTTPTDAAGPRVVSLYGDTASSLRPAPDQ
                     LEGLDVLVIDLQDVGARYYTFQATMLYALEAAHAVGLRLLVLDRPNPLGGVVVEGPRL
                     MPGYESFIGAHDIAIRHGLTIGELAALYRSEKRLENAGLEIVPCEGWKRSMSYADTGL
                     PWVLPSPNMPTVETALVYPGQCLLEGTNLSEGRGTTRPFELCGAPWIDAPALETQLNG
                     RNLPGVGFRAAWFQPTFQKHAGCVCGGVQLHVRDRAAFASVRTGLAVLETLRSASGPR
                     FAWRTEPYEFVADPIAIDLLFGSSRERLALEAGASCEDIVSAWTPEEAAFAERREPYF
                     LYSE"
     gene            14858..15886
                     /locus_tag="PKK20_00800"
     CDS             14858..15886
                     /locus_tag="PKK20_00800"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU98460.1"
                     /translation="MKSTLRLLYLLSTAACLAQESPDPYMGDWKGTMTIDGKTQPVCL
                     FLIPHGSQRYEAKLVSTFDKRVPTLFQLRGRIDDSGFQFVDAIPFEANRVVRTTDDGV
                     VIGASLWSGGGQDDSAYGTVAGRNKGSFEIEHIQRLSPTLGKQPPADAIVLFDGRSLA
                     AWRPRDPNAGEVKWKLVEGGAMQVNGGDIITRQNFGSFRLHLEFQLPYMPEARGQGRA
                     NSGVYLQGRYEVQVLDSYGLEGEDNECGGIYQIARPILNMCAPPLQWQTYDITFTAPK
                     LDAAGKKTANARATILLNGVVIHDNLELPRITGGAVDNKEGRPAGILLQDHGNPVRYR
                     NIWIEKLD"
     gene            complement(16028..16939)
                     /locus_tag="PKK20_00805"
     CDS             complement(16028..16939)
                     /locus_tag="PKK20_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98461.1"
                     /translation="MRIAQYEQTSSSDPAPAPETPFHFNAMVVSPRNGPAVTSGSVTL
                     PNGTVKNLEGESMIGFLMYTEDFGSDAALEAAYPAGKYTLRFTQTGQAERAIAMTMPT
                     ATVPVPKIANYTEAQSVNHAQDFTLRWNAFTGAGANDHLSLIIFDRNVGGVVFRAPDL
                     CVPRELPVSATSIVLPANTLQNNRTYEATLMFGRMFYFSTNAVPEMSGSGHIGRQTTF
                     TVKTGTGGTGAAATFTGCRMLPNGNPEMTLAGTPLRTYTIQRTGTVGAAVWTQVGSVT
                     MNAAGTGVFEDAQAGKSLPLFYRAVAN"
     gene            complement(16955..17821)
                     /locus_tag="PKK20_00810"
     CDS             complement(16955..17821)
                     /locus_tag="PKK20_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU98462.1"
                     /translation="MNGSSCSVGCGARGAAAVIRTAALVALSFLLGCNLALAQTAPKI
                     VSVTPANGASDVSVTTQLVFVFDQPMLTWVPIIPSIPGVFVGNLDLSPAESVPSLNGL
                     WSEDGRTLTCVPSSDWPPNTRIRWRLNPAGVLVPITSESGVDLVEVSGSFTTGAGGGG
                     GTEPALVASTPSNGAMGVAVNTPVTFVFDQPMKKIAVIGDAVEWQGDGVDPAKFVYSW
                     SADGQTLTCSHAGGFPANTVIAWTLNPESSSVRFESETGEALPEGTYSGFFKTAQDGG
                     DDDCTPDGIPES"
     gene            17940..20264
                     /locus_tag="PKK20_00815"
     CDS             17940..20264
                     /locus_tag="PKK20_00815"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HNU98463.1"
                     /translation="MKTILRSVASLAAMLTAANSTVAAPAFEIAQWIAPPVATNRPAL
                     PIFRKEFSIAALPDQATLRIVGLGDYDARINGRRVAETGINQPWSQYEKTLYYRDFDV
                     TSLLAPGANCIGVMLANSFWDNPNPPPGRYNKGGPQRAADEPFRLCVELILRGKDGTL
                     ERVGSDAAWRTTEGPIVFSHIFAGEDYDARRRLTGWDRAGFDDRGWSSARRLSPLSAA
                     LERQFWSGFAELDRFQPVSVSEPAPGVFLYQFAQNCSAQMRFRMTGGKSGDRVSFRCG
                     EHKNNQDRLFGGYVVGCDAVTDGQPFEHQWISFYLGMQFVEVSGAVPAGHPNPDGRPI
                     IESLELRHVRAALPEAGTFHCSSELYNDTYRIIDWAMRANTSHVLTDCPHREKLGWLE
                     CAYLLAPSFQHRYDCRDWMAKLTRDIRDAQKPNGQVLTVAPSYPGGRFGGGFDYTLEW
                     GAAAVMLPWHHYQAYGDPHILRDNFNAMKRFTDFVATTAKDGIAAGGLGDWYDYGHGQ
                     PPGESRFTPTDLSATATWALCALTVSRAAEALGRATEADAYRERHARIAADFQRRFRD
                     PATGKLRHTGSPQCANAMALCAEIVPPNDRAALVDEIIGDLRRRDWQQTPGDVGHVYF
                     IRALAEAGRSDILHRVYSRQDKGSYGGILAKGLTALPETWDAMMDGSQSLNHCMLGHV
                     IEWFHEYVAGLRQPPGSVGWQQALIAPCPGPLTSAEATLRTPRGRIASRWHKDAGRFR
                     LEAELPEGIAAQAIMPSGTRHPLSPGFNAIEEPL"
BASE COUNT         3764 a         6521 c         6558 g         3627 t
ORIGIN      
        1 ctccggttcc agagcccgaa cctttagatt cttgagaaag gaaacgggcg gatcggcact
       61 gaacagttgg tggtttgtcg agatcatcgc acgcctacta cgcaccaaac cacgccaatt
      121 gataagcgtt atttctaacc tcaacaacag caacccttca ggaattcaaa tgaaataacc
      181 gtgcttgacc cccttcaacg cagcacagaa agctgacttg tttacagcga gtttcgccgg
      241 tcgaggattc tattcgggtt tgaacccggg ctgtccggcg gtctctgttg gctggatgag
      301 ccagttctgg gcgccggagg tctgacgggc ggcgctcatg gcggcttggt atgccggctt
      361 gttggatcgg gcgatccaga gccgattcgt ggcgaatgtt agcatcggca agtctccggg
      421 actgtcgccc gcggcgagga acggaggctg tccgatcacg tcccagagag cggcgacctt
      481 gcccgagtag actggcgcag gaaagtgaat ctggtccgaa atgcggaatc gagcgagcgc
      541 tcgcatgttc atgtccgcgt aggcgggatc ggtgcggacc aggacagaat ctttgtgaaa
      601 ccgttcgtgc cggtccacga gcagagtcga gacgccgatc acgtgatcgg cgcggatcgg
      661 gatacgggca cggagggagc gcagcagtgg attgagtgct tcgaggacca tccaccggac
      721 gctccagaca ttgcttgccg agacgatcca gacgtcgaaa ccgctttgga gcaacacggc
      781 gacgaggtcg atcatttccg ggtagaagaa ggggatcggg taaccggtcc gcccgggagc
      841 ggtcgtgatg aaggtgttgc gtccagggcg ggagagcttc cagacctctc gggtggcgcg
      901 cgccacgtcc tggacgctca atccctgcat caccgtcgcg gcccacacgt atccgttggc
      961 cagcggagtc ggatcgagcc ccccgtgggc cgtcgggtcg agtaacgcct cgtaatagtc
     1021 cgtcagatcg gcgccggagc agggcgagat tcgccggccg cccctggatc gaaactcggg
     1081 agcgatgtgg gggggcaggt gctcggctcg aatcagcccg tcgcgctcga gcaaagccaa
     1141 agtcgcctcg ccaatgtcgc cgcagaccag ggtattgtcg aagtcgaacg cggccgaccg
     1201 atggcggcct gccccggtcc gaatcaacct ttctagccgc cggcgggttg cgggctgcca
     1261 accgcggggt tcaaggcgga gggcgtcgtt catttcactt ggctcctttc ggacgtgcat
     1321 tccagatggc gatcataagg agtgcggaga caacggcaag gccggcgatg aagtggaaga
     1381 ccagcgccca gtcgccgtgt gcgcggtcct tcagccagcc gatgccggct ccggagaaga
     1441 tcgcgccggt ggctcccagg cacatggtga ggcctgtggc gatggacgcg gctttgggat
     1501 ggctgacgtc gacggtggcg gcgccgctca tgagcatgtc gggaccgtag ataaagaacc
     1561 cggccaatcc catggcgatg gtggcccaga cccattctcc ctggggaatc caggcgaagc
     1621 ccacgcagac tccggccagg gcgatgagca tgatggcaca gaccggcgcg cggcgttttc
     1681 cgaagagggt gtcagaggcc catccggcgg cgatggcgcc caaggatccg atcagaggga
     1741 tggcgacggc ggtgagggcg ctgcccttga tgctgcgccc ctggaagtcg gccatgtact
     1801 ggacgctcca gttcatgaag gtgtagcgga ccgaattcat gcagaagaag ccgacagcca
     1861 gcacccagag aacacgattc gacaaggtga gggccagaat ctgccgtgtg gacaaggcgg
     1921 cggcaggggt tgcggcgcac gcgggtcttg aagcctctgc cggggtgtcg tggccgacgt
     1981 cgtcccggac cggcgcgaag cctgcgtctt cgggcgcgtt gcgaaggaag gcgaagaagg
     2041 cggcggctat ggggaggaga atgaggctgg ggacccagaa ggcggcgcgc cagccgacgg
     2101 tgtcgcagag catccccgcg agcaaccagg agacgacatt gccgacctgg tagcaggttg
     2161 agatgaggcc gatgacggtg ccgcggcgtt cggacgtgtt ccaattggcg attgtctgta
     2221 ccagaagcga ccagccggcc gactgggcgt atccgttcac gccccagagg gcgagcatga
     2281 cggggaaaga cgatgtgcgc gaaaaaagca ggttggtgaa ggaggccgtc aggagggcca
     2341 tcgtcatcag gtgacgggcg ccgtagcgtt gtccgagcgt gccattgacg agctggccgg
     2401 ccgcgtaaca ggccgcgtag acggaaggaa tggtgccgat ctgggcggcg gtccagtcgg
     2461 tgaactcctt caacatcatt ggctgggcga cggcgaaatt gacgcggcac aggtaatagg
     2521 atgcgtaggc gccccagagg agccagaaga tgcggagctg ccaagcccaa aggcttgcgg
     2581 cgggagcaga agtgggcggc atagggcgtg agatcgagtg cgcgctcaag acatgttggc
     2641 ccgcccgtta tggccatttc ctcgacgcgc gtcaaattga gtcgacgcgt ttattgaaat
     2701 cgttcgttca ttgaaatcgt tgttgaaatg tcaaacgact ctcttcatac tctgcccgag
     2761 cagccgaacg gtgacgaagc gcatatgatc atttctcgcg ccccgatggt ggttgaaccc
     2821 tcaacctggg agaggaggtg agacgacgtg gccgcagcca agaaacctgc caagaaaaca
     2881 gccaagaagc cagttaagaa ggctaagaag aggtaacctc atccacgttg gtccaactcc
     2941 aggccgccaa agtgcggtcc caatcaagcg accgccgcgg cttggagttg gccttctttt
     3001 tttatggcga ctctggtctt cgatatcgag acggcagctt gttcgcgcga ggagtttgac
     3061 gatgcgcagt gggagttcct gcaccgcgat gcggagcgcc tcgccgatcc cgccgcccgc
     3121 gaggcgcgtc gcgccgagat ccagcaccaa ttcagtctgt ggcctctcac ggcgcgggtg
     3181 gtctgcatcg ccatgctcaa cgcagacacc gcgcgcggcc aggttctgtt caccgcgagc
     3241 gactacgacc cgggcgaggg tgaggggggc gccgagccgg tggagtttgt cgcctgcatt
     3301 gacgagtccg agatgctgac tgcgttttgg gatgtagcca ggcattacga tcagattgtg
     3361 acattcaacg ggcgcggctt tgacgtgccg tttctttacc tgcgctcggc tctggtgaac
     3421 gtgccgatca cccgcaagga atggctggga taccggttcg ccaccgaacc gcactgcgac
     3481 ctggccgagc aactcagctt ttacggcgtg agcggacgcg atggggcggc gcgccgattc
     3541 aacctcgatt tctactgcaa ggcgttcggg atcgattccc cgaagagcca cggcgtcacc
     3601 ggaatggatg tgggacgcct gacggccgag ggacgccacc gcgagatcgc ggaatactgt
     3661 ctgcgcgacg tccaggccac cgtccatctt tacaaaatct ggcgccaacg cctggcgggg
     3721 atcaagtgac ctgccggacg gccacggtca gaccttgaca acgagacgcc cgccggtctt
     3781 ctcgacgcca tgattcgtct ggtcggctgg cccgacggcg ccggcaaccg ccatgtgctt
     3841 gtggccgcat cgcacgacga agccgcggcc tcgccgcgga gcgccatcct cgactcgctg
     3901 gttgcgaacc caggcgctgt tcagtcctga ccgtgcgtca atgccagcct cggaccgatg
     3961 gccacagccg acatcgacgg cgatggcttc ggggatttgt ttctgggcca gaacttcttc
     4021 ggctcgggtt ccgatctgac ccgcgactga tgtcgccctc gaacggcatt cacttcgtcg
     4081 gattccgtgt cgccctcggc ccaggagcgg caaggtcaag tcgttagcgc gagtctcaag
     4141 cgcgacgctg ggcgaggcga tcgaggagtt tctggtgcag cccgccaaaa ccgccgttgc
     4201 tgaagacgca gaccacatcg cctccctgaa cgctgccggc caggtgctcg acgatcgagt
     4261 cgacgtccgg caggtaggca gccgtcttgc ccgcggcctg gagatcggcc atcaggcgct
     4321 cggggtcgag gcgttcgtgg ggcgccagca gttcaagtcg ggacacttgc gcgatgacaa
     4381 cggcgtcggc gcccaggaag gcgtcggcca gttcgcgctg gaacacgttg cggcgggtgg
     4441 tgttggtgcg cggctcgaac acggcccaga gtttctcggc ggggtgcttg aggcgtaggg
     4501 ccttgagggt ctcgcgaatc gccgtggggt gatggccgaa atcgtcgatc accacgatcc
     4561 cgccggcgac gccgcggact tcgaggcgcc gcttgacccc tttgaaggtg ttgaaggctg
     4621 cctgaatctg cgcgttggtc aggccgcagt gccgggcgca ggcgatgacc ccgagggcgt
     4681 tgcgcacatt gagttcgccg atcaggttga ggtggtactt gtggttggga gtttcgaact
     4741 cggtggcggt cgctcccagc cgcagcttga aggcccgcaa atggttctcc tcgccaaaac
     4801 cgaaccgttt gatcgggcag tgcttgaatt cgaggagcgg ctcgagattc ggatcatcgc
     4861 cgttggccaa aaccagtccg ttgcgcggca ccagccgaat cagatgcgag aaactggtct
     4921 ggatcgattc gagatcgcgg aagatgtcgg catgatcgaa ctcgaggttg ttgatgatcg
     4981 cgacctcggg gaggtaatgc acgaacttgc tgcgcttgtc gaagaaggcc gtgtcgtact
     5041 cgtcaccttc gatgatgaac cagtcgctgt cggtgaaccg ggcgccctgg ctgaagttgt
     5101 tcggaatgcc gccgatgagg tagctgggat tgagtccatt gtgctcgaac acccaggcca
     5161 gcagcgcggt cgtcgtggtc ttgccgtgcg tgcccgccac cacaagcgag cgcttgcccc
     5221 ggatgaagta ctcgcgcagc agctccggca gcgagcagta ccgcatcctg tggccgagca
     5281 ccgcctcggc ctccgggttg cctcgcgaga tcgcgttgcc gatcaccacc aggtccggcc
     5341 tgtgcgccag attccgctcg gcgtagccgt tcatcacctc gaccccgcgc tcggccagaa
     5401 aggtcgacat cggcgcatag acgttctcgt ccgaaccggt caccacatat ccctggtcct
     5461 gcatcgcgcc cgccaccgag gccatggccg tgccgcacac gccgacaaaa tgaaacgaac
     5521 gaatctcggg ccgaaacatg ccgtccatga tgcgtccgcg cccgacagtg aaaagcagaa
     5581 aagggaaaat ctctttgatg agcgaggcgc gccctcggac caatccaaat ccccgtcctc
     5641 atgaacggcc cttcaggact ctctgcgcct cgccccgtgc catgctgggt gcggggcccg
     5701 gcgtcgctcg agatgctctc gccgcgcgtc cagccgccgc gcctcactgc gctgggcgga
     5761 agcgtcgaca cgccgccgga gggaatcacg gcggacctgc tcatcgtgcg cgacttcacc
     5821 gaactcacca accgcgcccc cgaggcccgc gctgtcggaa gcctgatcgg ctccccgact
     5881 ccgttttcga tgcaaacgcc ccagacgggc atgatgcggt acgaggacgg tgtcccgcga
     5941 atccctcacg ccgccctgag tgtagaggac ctgctgctgt tgcaacaaga attgacccgt
     6001 tcaaagattc atggccgggc gtgccgaatc cgtgtaacct cggggtgtcg attcctcagg
     6061 aaagggcggc atgacaaact cgatgccacc ttctcactgt cccagttgcg gggcggaagt
     6121 gccgttcgaa gcgccgcagg ggctgtgtca ccgctgtctg ccggccggcg ttgcgcaagc
     6181 gaccgagtcg gcgccaacgg ctcggaactc ggatgggccg ccatcgatcg atgtggtgaa
     6241 gtcctcgttc ccgcagttgg acatactcga atgcatcggg cagggggcat gggcgtggtg
     6301 cacaaagccc gccagcccaa gcttgaacgc cttgtggcgc tgaagctcat gcagtcgtcc
     6361 aaacggtgcg cgacagagcg gatgtcgggg ccgaactcag gcacctgatc gatgtcgtgg
     6421 cgacgggggc gtgaggcgcc ggatcgggat gaaacagccc ggtatcaaac ggaatgaaac
     6481 ttgacttgac cgcagatctg gagtttgagg acctccaaaa acaccatgac aacacacgac
     6541 atcatcaatc gacgccactt catcgagact gccggagtcg cggctgccgc cgcgggattc
     6601 ctcggaagcg cagcgcccgc ccgcgccgct gatgcgccgc ctgcggcgaa cgcgaaggtc
     6661 aggatcgtgg ggattagcgg gagccatcgc aaaggcaaga cgaccgccac ggggcttcaa
     6721 atggcgctcg attccgccaa ggccgtcagt cccgagatcg agaccgaact gatcgagttg
     6781 atggactaca agttgctccc ggctgtgccg ggcggcgaga tcgaggacga ctacgccagg
     6841 attcacgcga agatcaccga cccgtcggtg gggggcatca tcctggggtc gcccgtgtat
     6901 ttcagcaaca tgagcgcgtt gatgaagatg ttcatcgaac acttcgggcc ctacaagaag
     6961 agtggggcgc tctcgaacaa ggtcgcgggg gtgttgacag tcgccggcgt gcgcaatggc
     7021 ggccaggaag gcgtcgccca gtcgattatg gctgcggccc tgggcgtcga gatgatcgtc
     7081 gttggcgacg ggcgtccgac aggccacagc ggcgccacgc tatggaacaa cgccaaggac
     7141 gacatcacgc aggacgaatt cggatgcagc acggcgaaag gcctgggcaa acgggtcgcc
     7201 gaggtggcgc tccgaatggc aggcaaggcg aggtcctgac ctgaaaactg cacacagccc
     7261 ggatttctca ccaatggaaa ggcgtaacgg aaatccgggc tgcggcacaa ccgcgcccgg
     7321 gtccgggaga tcgggaaatg tgacaggccg ttcccatggg cgaacgcctt cggaaccgac
     7381 aggaggctct tggcaaagcg gtgtcggtcg ggcatgttgg cgttatgcgg accgcggggg
     7441 catttcactt gcgcgagacg acggggacgt cgggtctcga ggcggcgctg cgcagtgcgg
     7501 tcgaggcact ggcgcggcac gaggtgccgc atcttgtcgt tggcggcctg gccgtccagg
     7561 aacacggcta cttccgcgtc acgacggact gcgaccttgt tgtgccggac gtgttggatg
     7621 cggtcgaggt gctggtcgcc gatctgtcgg ggtcgttcgt caagcacggg gactgcgacg
     7681 acacagtgaa atacaagcgg aacggggtgc tgatcaacct gctgccggcg gggcatgtcc
     7741 tcaaggcggg ctgtcgggtg ccgtttcccg tgcccatcga ggtctgggac gagccccggt
     7801 ttgtctcact cgaaaagttg atcgcgctca agctcgacag ttggtcgaac agccccaacc
     7861 gccggcacaa ggacaagacc gacgtgatcg agttgatcca agccctgcgt ctccctcggg
     7921 acctggcggt ggacgaatcg gtccggaccc tctatgtcga gacctgggac gcgctggcgg
     7981 cggagcctgg ctagtccgca taggagtcct cttgcgaagc ctcagtgccc tgtgcaccga
     8041 cggggtgcga aatatgcagg ctagaaaagc gtcgggaaca cctcgatgag gtcatcgatg
     8101 ttcgctgcgg ttacgaacaa gtaccgggct tcaaactcgt cgcggtcatc tcgctggatc
     8161 gcggcggttt ccgggggcgc gcccgggcga ttgaggacgg cggcgacgat cttgccggac
     8221 ttgaaagcgg cggcgatggc ccgcgcatcg ccgatcaggc ggaggtcggg gaggaggagg
     8281 gcgaatcgga gagtggggtt gcgccagcaa tccagggcct gcaacccggc cggaagcccg
     8341 atgacgctga cgaacagggt ggcgtccggg tgttttaacg cgagtgcgtc ccatgcgccc
     8401 tgggcggtga gaaaactgag cggcgtcgtg gtgtcggggg gcagcgggac cgaggatgga
     8461 ttctgtatgg ctgcgggagt cagttccgga taatcgactc cgactaaccg gattcggttg
     8521 ccccaaccgc tgcggagtcc gcggatggct gcctcctcga aggcgtagac cgcgcgcgcc
     8581 tggccgggca tttgcccgaa cggattcgcc acgacgagcg ctgcctggcc gggcgtctgg
     8641 ccggctaggt gttcgcccag aacgcgcaag gcgatttcgc ggcggtccag ggcgcgcccg
     8701 cgcgcgaggc ggtcgggagt gcggaacatg cgccagacga gcagggcggc gacggcgacg
     8761 aggaccgcga caaggacagt ttttgctttc atgtggctgc ttcactggcg ccgacgagga
     8821 ccgccgttta gaatccccgg gaagacggcg gaatggttgt gaaccgttgc cccactcacc
     8881 cgcctccgcc gaagtagcca gggatggtgt cgacctcgaa gagagcggcg atgcccaggc
     8941 tctcgaggcg tttccgattg cagccttcga cgtgcccatc gaggaacagg ccgttggccc
     9001 tctgcgaatg gcgcgcatgc acttcattct cgttggccgc gcggaagaag tagaattgtt
     9061 gcgagccgat ccccaggcgt ccgcggctgg tggtatcggc cacatggagg tactcgagcg
     9121 gatttgcgat gctgtcgacg tggaggagtt cgctgaaggc gcctcgggtg taattggtgg
     9181 gcgggtccag acgcacgccg taggtgcgaa tccagttggt gagcttgggc ggggggtatg
     9241 aggggcagag gaagagttcg aggggacgga ggttttcgtt tgtgctcagg agcgaggccc
     9301 aggtgacgcc gggatcgagc ggggcattga tgggcaccaa accctggttg tcctgggcgt
     9361 aggcgagcgt ggccaggccg atctgcttga gattgttgac gcattggact tgcttggcgc
     9421 gtgccttggc acggccgagg gctggcagca ataagctcgc gagaatgccg atgatggcaa
     9481 tcaccacgag gagttcaatg agggtgaacc ccgttgagat tcggcggcgc atgcagttcc
     9541 tgttccgtgc gggcgcgtgg ccgacctgca atccgcactc cggcatccgg agtgcgtgtc
     9601 cgtgttttcg ctcgccaccc gcatcgatcg gtctagcata tcgcaggcca tgattgggtc
     9661 aatccatcgc catgttgttt aactcgggtg tctttctcca gttcttcgcg gcgttcctgc
     9721 tgctctatta cctggttcga aaccgcctga tggcgcggaa tggcctgatc gtcctggcca
     9781 gctacctctt ttacggctgg tgggattatc ggttcctgtc gctgttggtc ttctcgagcc
     9841 tcctggatta cgccgtgggc cgggcgctgg atcgctgcac gcatccggga caccgcaaac
     9901 tctggctggc cctgagcctc gccacgagcc tcggtctgct cggtttcttc aaatactaca
     9961 acttcttcat cgactccgcc gcagtcctgc tcgcgcaact ggggctgtct gtcgaacccc
    10021 gaacgctcga catcgttctg cccgtcggca tctcgttcta cacgttccag accatgagtt
    10081 acacgttcga tgtgtaccgc cgaactatgc cggcgacgcg cgacctgctc agttttctcg
    10141 cgtatgtcgc gttctttccg caactggtcg ccggcccgat cgagcgggcc agccgcctct
    10201 tgcctcagtt caccaaaacg cgcgtcatca cacgccagcg gctgatcgag ggcacgtggc
    10261 tgatgatttg gggcatgttc aagaaggtgg tggtcgccga caacctcgcg ccgctcgtcg
    10321 agatggtgtt cgatcatccc gcgccgacgg gactcatggt catactcggc acggtggcct
    10381 ttaccttcca aatctatggc gatttctccg gatactcgga catcgcgcgc ggtctggcca
    10441 gcgtgctcgg cttcgacctg atgctcaatt tcaacctgcc ctataccgcc accagcccgc
    10501 gggatttctg gaggcgctgg cacatcagcc tgtcgacctg gctgcgcgat tatctctaca
    10561 tcgccctggg aggcaaccgc cgcggagtct ggcggacgcg attcaatttg ctggcgacca
    10621 tgcttctggg gggattatgg cacggagcgg cttggagctt tgttctttgg gggctctggc
    10681 acggcctggg cctcgtcctg acgcgccccg cccaatcgca cgtcccagcg cccgaccgcc
    10741 cccggtggcg gctgctgcag tgggcggcca cgatgctctt tgtgctgtat ggttggctcc
    10801 tgtttcgcgc cgggtcgctc gcgcacatcg tcgagctgac ctccgccctg gcctgttggg
    10861 agtggccgac ctggggactg agttatctga tcaacctgat ggccttctcg ctccccttgg
    10921 cgctcgctga agtatggcag gcccgcgcgg gcgatctcat gaccccagcg cgctggctcg
    10981 cctcgatgag ggctctgctg caaggcattc tgttgctggc gatcgtcgtt ttctggcaaa
    11041 aagaagccgc cccgttcatc tactttcagt tctgaggcac ccatgaatcc tcaagattca
    11101 atcggcggca catccaccaa ggccatgacc accgcacaga cccatctgcg cagccaccaa
    11161 tgcgccttca cggcgcgcga tttggccgag atcgtcggcg cgccgctcgg tgtgctgctg
    11221 ctgctcgcag cgctgtttca ttttgcggcc gccgcggact ggctgccggc gccccgaccg
    11281 acactcgaca tggatcgcac cctgctgatt caccaggcgg actcgagccg gtcggcccag
    11341 gacgcgacgg tcatcctgat tggcgattcg tcctgcctga tggatgtgtc ggcgtcggac
    11401 ctgacacggc acttgggcca gccaactctc aacctgggcc tcctcagcta cctcgatctg
    11461 cccagcttcg gcatccttgt gcggaactac gcccatgcga accccgagcg tctcgagact
    11521 gtcgtgttgc tgctccaccc ggaaagcctg cggctccagg cgccgtcggc ctatcaccgc
    11581 gcccaactcg aggactatct ggccggacgc gacgctcaca cggctggagg gatcgggggc
    11641 cggttgcaga cggccctggg cgccatcctg gtgcgcggga ggctcgaagc ccgggtcatc
    11701 cctgccccgc tgtccggcgt gtatggccgc ttctacggtt ttacccgcga cttctcacgc
    11761 tggttggggg cgcaccgggg cagcgcgatc gatccgcaac gcttcgaccg caccagggcg
    11821 caaggtaacg ctgaatatcg gctggccccg cggctcgaat ccgaaagccg tctgctgcgc
    11881 caatgcgtgc cttcgggcgt gcggctggtc gtggggctga ccccggcccc gagatcattc
    11941 gttcgccccg accatgaggc cacgtgcgcg cgcatcctcg aacaattggc caagtggttg
    12001 aaagccgatg ccgtgctcgc cggtcttcca gcctcgatgc ctgatgaact gttcgcgagc
    12061 gtcacgcacc ttaccggaga ggggcagact ctgtttaccc agcgcctggc cgcggaaatg
    12121 caaaccgccg ccatcgtccg gacaccgagg cccgactgga ttccccgagc tgaacacttg
    12181 atgcaggaat gaatcgtgct aacaattctc tcgtggtcag tgattcgaac ggcgatcgca
    12241 ggcagttgcg catggtgcca tgcccgaact gcggagtgag gctctttctg gacggagacc
    12301 tcccgccgtt gagcacggtg ccctgcacca aatgcggcga gtcggtcatg ataccgatgc
    12361 gcttgcggca gttcgaattg caggccgtca tcggaaaagg agggatggcg acggtctacc
    12421 gggcattcga tacgacgctg caaagggatg tcgccgtcaa actcatgaac cccgagctga
    12481 tgcaggatca gaacgggatg aaccagttcc tgcgcgaggc gcgggcgtgc gggcagctca
    12541 accacaccaa catcatccac atctacacct tcgatgacct ggatggacag gcctacctgg
    12601 tgatggaact ggccgatcgg gggagcctcg acagccgaat cgagaaaggc cccgtgtccg
    12661 aactcgacgt gctcgatatc ggcatcaaag tcacctcggc gctcgatacg gcccttaaac
    12721 acggcctcct gcactgcgat atcaaaccgg gcaatatcct gttcaatgcg gagggcgagc
    12781 ccaaattggt cgatttcgga atcgctcgcc gcacggacgc cgaatctgac cgcgatggaa
    12841 ccgtgctggg cacgccgtac tacattgcgc cggagaagat ccggcgggaa caagagaact
    12901 ttctttcgga catgtacagc ctggcgggca cgctttacca tgcgatcacg caacatgtgc
    12961 cgttcgaggc gccgacggtc gaagacgtgc tcgccgccca cgtgcacacg ccgcttacgc
    13021 cgcccgatcg cgtcaacccg gacatcacag cccccaccag cgacgcgctg gtccgcgcga
    13081 tggccaagaa accgtccgat cgattcgagt cgtacgatgc cttcatcatg gcgctcaacg
    13141 ccgcccgcag ccagtacctg gtcggacgct atatcaatcc tcagcccgcc cggcccggga
    13201 gccgcctcaa gaatctgttc cgccggtaac cccatgaacc accctcaccc ccaccagcac
    13261 tgctccggtt gtctctccca ctgacgcagc ctgcaactga ggcggcgagg ggagaatgtc
    13321 caaactcctg tgcctgccgc ctgcctgccc tgcctgcgcc acaaagcgca gcggactgtc
    13381 tgccgtgtcg ggcacggcac aggcaggcgc ggcaggcagg cccaacgcgg cgcagtttgg
    13441 attcccgcct acaacctctt gaggcacggc gacggaatcc gttgcccgcc gggcaggggg
    13501 gcttattcag agtacagaaa atagggctca cgtcgttccg cgaaggccgc ttcctcgggc
    13561 gtccaggcgg agacaatgtc ctcgcaggac gctccggcct cgagtgcaag gcgctcgcgg
    13621 ctgctgccga agagcaaatc gatggcgatc gggtccgcaa caaactcata cggttccgtg
    13681 cgccatgcga accgcgggcc cgatgccgag cgcaacgttt caagcaccgc gagtccggta
    13741 cgcaccgatg caaaagccgc acggtcgcgc acgtgcaatt ggacgccgcc gcagacgcat
    13801 ccggcgtgct tctgaaaggt gggttggaac catgcagccc ggaaaccgac cccggggagg
    13861 tttcgcccgt taagctgcgt ttcgagggcc ggcgcgtcga tccagggcgc gccgcacagt
    13921 tcgaacggac gggttgtgcc gcggccctcg gagagattgg tgccttcgag caggcattgg
    13981 ccgggataaa ccagggcggt ttccacggtc ggcatattcg gtgaaggcag cacccacggc
    14041 agtccggtgt cagcgtagct catcgaccgt ttccatccct cgcacggcac gatctccaga
    14101 cccgcatttt cgagtcgctt ctcgctccga tagagcgccg ccagttcgcc aatcgtcagg
    14161 ccgtgccgga tggcgatgtc gtgggcgccg atgaaactct cgtagccggg catcaggcgc
    14221 gggccctcga cgacaacgcc gcccagcggg ttgggccggt cgagcacgag caggcgcagc
    14281 ccaactgcgt gggcggcttc gagcgcgtag agcatcgtgg cctgaaacgt gtagtagcgg
    14341 gcgccgacgt cttgcaggtc aatcaccaga acgtccagtc cctcgagttg gtccggggcg
    14401 gggcgcaagc tcgaggccgt gtcgccgtaa aggctcacca ctcgagggcc tgccgcatct
    14461 gtcggggtcg tgctcgggac cgcttcgagg tcctgggcgt ggcccatcca gccgtgttca
    14521 gggccgaaca ggacctcgag acggacgtcg ggcgccgcca acagcaggtc ggcggcatgg
    14581 cgcaatcggg cgtccacgga cgccgggtgc gccaccaatc cgacgcgctg cccgcgcagc
    14641 cgcttgaatc cctcagccac cagcacgtcc aggcccgttc gcacgcgagt catgcgcctg
    14701 agtctgccag gacacgcgcc gaccgcccag cgtcaattca cgataacgct cagatgcacc
    14761 gcgtccgcca ccagagcaga gagtgcttga cccgccagcc cggcacttta gcctttcgct
    14821 tcgcaacaga acacgcgaca cgacatcccg tttgactatg aaatccactc tgcgtctcct
    14881 ctacctcctc agcaccgcag cctgcctggc ccaggaatcg cccgacccgt acatgggcga
    14941 ctggaaaggc acgatgacca ttgacggcaa gacgcagccg gtctgtctct tcctgattcc
    15001 gcacggctcg caacgctacg aagccaagct ggtctcaacg ttcgacaaac gagtgcccac
    15061 cctgttccag ctccgcggtc gcatcgatga cagcggcttt cagttcgtgg acgccatccc
    15121 attcgaggca aatcgagtcg ttcgaaccac ggacgacggc gtcgtgatcg gcgcctcgct
    15181 ctggtcgggc ggcgggcaag acgacagcgc ctacggcacc gtggcgggcc ggaacaaggg
    15241 cagcttcgaa atcgaacaca tccagcgcct ctcgcccacg ttgggcaaac agccgcccgc
    15301 ggacgcgatc gttctattcg acgggcggag cctcgcggcc tggcgcccgc gggatccaaa
    15361 tgccggcgag gtgaaatgga aactggtcga gggaggcgcc atgcaggtca atggcggcga
    15421 catcatcacc cgccagaact tcggcagctt ccggctccac ctcgaattcc aattgcccta
    15481 catgcccgag gcgcgcggcc agggccgcgc caacagcggc gtctatctcc agggccgcta
    15541 cgaggtgcag gtgctcgaca gctacggact cgaaggtgaa gacaacgagt gcggcggcat
    15601 ctaccagatc gcccgcccca tcctcaacat gtgcgcgccg ccgctccagt ggcagaccta
    15661 tgacatcacc ttcaccgcgc cgaagctcga cgccgccggc aaaaagacgg ccaacgcgcg
    15721 cgccaccatc ctcctcaacg gcgtggtcat ccatgacaac ctcgaattgc cgcgcatcac
    15781 cggcggcgcc gtggataaca aggaaggccg gcccgcgggc attctcctgc aagaccacgg
    15841 caacccggtc cgctaccgca atatctggat cgagaagctg gattgaagcc acgcaccggt
    15901 tcccgaaggc ggaagctgag tgggccgcac tatacgcgac aaagacccct cgccacggcc
    15961 ctctcccatc cgatgggaga gggtgacggt gtctcgtatc ctaccgattc ttctcgaaca
    16021 ggccgactca gttcgccaca gcccggtaga ataggggcag gctcttgccg gcctgggcgt
    16081 cctcgaacac ccccgtcccg gcggcgttca tggtgacgct cccaacctgc gtccagacag
    16141 ccgctccgac tgtgcccgtg cgctggatgg tgtaggtgcg cagcggtgtc cccgccagcg
    16201 tcatctccgg gttgccgttg ggcagcatgc ggcacccggt gaaggttgcc gccgcgcccg
    16261 tgccgccggt gcccgtcttg acggtgaagg tcgtctggcg cccgatgtgc ccgctgccag
    16321 acatctcggg cactgcgttt gtcgagaagt agaacattcg gccgaacatg agcgtggctt
    16381 cgtacgttcg gttgttttgc agcgtgttgg ccggaaggac aatcgacgtt gcggaaacgg
    16441 gaagttccct cggcacgcag agatcgggcg cccgaaagac cacaccaccc acgttcctgt
    16501 cgaagatgat caaggagaga tggtcattgg cgccggcgcc ggtgaaggca ttccagcgga
    16561 gggtgaaatc ctgcgcgtgg ttgaccgact gcgcctcggt gtagttggcg atcttcggca
    16621 ccggcacggt cgccgtcggc atggtcatcg caatcgcacg ctcggcttgc cccgtctgcg
    16681 taaaacgcag cgtgtatttc cccgccggat aagccgcctc gagggcggca tccgatccaa
    16741 aatcctcggt gtacatcaga aaaccgatca tcgattcccc ttcgaggttc ttcacggtcc
    16801 cgttgggcag cgtcaccgaa ccggacgtga ctgccgggcc gttcctcggg ctcacgacca
    16861 tcgcgttgaa gtggaaaggc gtctcgggcg ccggcgccgg atcagacgac gacgtctgtt
    16921 cgtactgggc aattctcatc agggaatagc tacctcagct ctcaggaatg ccgtcgggcg
    16981 tgcagtcgtc gtctccgccg tcttgggccg tcttgaagaa gcccgagtag gtgccctcag
    17041 gcagggcctc gcccgtctcg ctctcgaatc ggactgagga cgattccggg ttgagagtcc
    17101 aggcaatcac cgtgttggcc gggaatccgc ccgcatggct gcaggtcaat gtctggccat
    17161 ccgcgctcca cgagtagacg aacttcgccg gatcaacccc gtcgccctgc cattccaccg
    17221 catctccaat caccgcgatc ttcttcatcg gctggtcaaa aacgaaggtt accggcgtgt
    17281 tgacggccac ccccatcgcg ccattactgg gtgtcgaggc gacgagcgcc ggctcggtgc
    17341 ccccgccgcc gcccgcgccc gtggtgaagc tgcccgagac ctcgactaga tcgacgccgg
    17401 attcgcttgt gatcggcaca agcacgcccg cggggttcag gcgccaccgg atccgcgtgt
    17461 tcggcggcca atccgacgac ggcacgcacg tcagcgtccg accgtcctcg ctccacaaac
    17521 cgttcagcga cggcacggac tcggccgggc tgaggtcgag gttgccaaca aacacgcccg
    17581 gaatactggg gataatcggc acccaagtca gcatcggctg gtcaaacaca aacaccaatt
    17641 gcgtcgtgac agacacgtcg ctcgccccat tcgcgggtgt gaccgagaca atcttcggag
    17701 ctgtctgagc cagcgccaag ttgcatccca gcaggaaaga gagcgcaacc agcgcagcgg
    17761 ttcggatgac ggccgcggcg ccgcgagcgc cgcaaccaac agaacaagac gatccattca
    17821 tagagtgtca gtattctctt tcaattcttg ccattctaga aaccccgtcc cgtatggcca
    17881 gccccatctg cggcgctttt gtgctgcaag tctcgccgtc caggctcacc atgcagccca
    17941 tgaaaaccat tctccgatcg gttgcgagcc tggcggcgat gctcaccgcg gcgaactcga
    18001 ccgtggcggc cccggcattc gagatcgccc aatggatcgc cccgcccgtc gccaccaacc
    18061 gccccgcgct gccgatcttt cgcaaggagt tctcgatcgc cgccctcccg gaccaggcga
    18121 cgctgcggat cgtcggcttg ggcgactacg acgcgcgcat caacggacgg cgcgttgcgg
    18181 agacaggcat caaccagccc tggtcgcagt acgagaaaac gctctattac cgcgatttcg
    18241 acgtcacatc cctgctggcg ccgggcgcca actgcatcgg cgtgatgctg gccaattcct
    18301 tttgggacaa tcccaacccg cctccgggcc gctacaacaa gggaggcccc cagcgcgccg
    18361 ccgacgagcc gttccggctc tgtgtggaac tgatcctgcg cggtaaagac ggcacgctcg
    18421 aacgcgtggg atcggatgcg gcctggcgga ctaccgaggg gccgatcgtc ttctcgcaca
    18481 tattcgccgg cgaagactac gacgcccggc gcaggttgac gggttgggac cgggccggtt
    18541 ttgacgaccg cggttggtcc tcggcgcgcc ggctctcccc gctttcggcc gccctggaac
    18601 gtcaattctg gtccggcttc gcagaactcg accgattcca acccgtgtcg gtgagcgagc
    18661 cggcgccggg cgtttttctc taccaattcg cgcagaattg ctcggcccaa atgcggttca
    18721 gaatgaccgg cggcaagtcc ggcgatcgtg tgtcgttccg gtgcggggag cacaagaaca
    18781 accaggaccg tctcttcggc ggttacgtcg tcgggtgcga cgcggtgacc gacggccagc
    18841 ctttcgaaca ccaatggatc tccttctacc tcggcatgca gtttgtcgag gtcagcggcg
    18901 ccgtgccggc cgggcatccg aacccggacg gccgcccgat tatcgagtct ctcgaactga
    18961 ggcacgtgcg cgcggcgctg cccgaggccg ggacgttcca ctgttcgagc gaactctaca
    19021 acgacacgta caggatcatc gactgggcca tgcgcgcaaa cacgagccac gtgctcaccg
    19081 attgcccgca tcgggaaaag ctcggctggc tcgaatgcgc ctacctgctc gcccccagtt
    19141 tccagcaccg ctacgattgc cgcgactgga tggccaaact gactcgagat atccgcgacg
    19201 cccagaaacc caacggccag gtcctgacgg tggcgcccag ttacccgggc ggccggttcg
    19261 gcggcggatt cgactacacc ctcgaatggg gcgcggcggc tgtcatgctt ccatggcacc
    19321 actaccaagc ctacggcgac ccgcacatcc tccgtgacaa cttcaatgcc atgaaacgat
    19381 tcaccgactt cgtcgccacc acggccaagg acggcatcgc ggcgggcggc ctgggcgatt
    19441 ggtacgacta cggccacggg cagccgccgg gcgaatcgcg gttcaccccc actgacctct
    19501 cagccaccgc aacctgggcg ctctgcgcgc tcaccgtctc gcgcgccgcg gaagcgctcg
    19561 gccgcgccac ggaagcggac gcttaccgcg agcggcatgc ccgcatcgcg gccgattttc
    19621 agcggcgctt ccgcgatccc gccacgggca aactacgcca caccggcagc ccgcaatgcg
    19681 ccaatgcgat ggccctctgc gccgagatcg tcccgccaaa cgaccgcgcc gccctggtcg
    19741 acgagatcat cggggatttg cgccggcggg attggcagca gacgcccggc gatgtcggcc
    19801 atgtctattt catccgcgca ctggccgagg ccgggcgctc ggacattctc caccgcgtct
    19861 acagccgaca ggacaaaggc agctacggcg gcatcctggc caagggcctc actgccctgc
    19921 cggaaacctg ggacgcgatg atggacggca gccaatcgtt gaaccactgc atgctgggcc
    19981 atgtcatcga gtggttccat gaatacgtcg ccggcctccg ccagccaccc ggcagcgtcg
    20041 gctggcaaca ggcgctcatc gcgccctgcc caggcccgtt gacctcggcg gaagcgacgc
    20101 tccgaacacc ccgcggacgc atcgccagcc gctggcacaa ggacgctggt cggttccgcc
    20161 tcgaagccga actccccgag ggcatcgccg cccaggcgat catgccatcg ggcacccgac
    20221 accctctctc acccggcttc aacgcgatcg aagagccgct ctgaacgttc gcgtgaggac
    20281 ttgattcccg actcaactgt gccaatcgcc cacctgactc agacgcccgg tgacgtcgtt
    20341 gaggttccgt tcgctcgcgc cgttgatgta gagttccatt gctccgttgc catacactgg
    20401 aagttccatt aagacctgat gccggaatcg tggtttcggc cttatttttc aaggggttta
    20461 cgcacgtggt
//
LOCUS       DAOQRO010000010        19666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1908_length_19666_cov_5.944011, whole genome shotgun sequence.
ACCESSION   DAOQRO010000010 DAOQRO010000000
VERSION     DAOQRO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19666
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1908_length_19666_cov_5.944011"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1276)
                     /locus_tag="PKK20_00820"
     CDS             complement(131..1276)
                     /locus_tag="PKK20_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNU98464.1"
                     /translation="MFKGAIEKEFGWEGDHLNDPYALCCLAFAFTMILAGRCQDKFGP
                     RLTASIGGLLVGTGMVLIATTTNYAIWLLGFGLLVGIGIGFGYSSATPPALKWFPPSK
                     TGLVAGLVVAGFGLAPVYLAPTSQYLLAHFALQKSMLIFGIAFVILVCALAQLLVNPP
                     PGYVAGGTPAGAGSAAPKPAASNVTPGEMLRQPLFYLLWIIYFIGAGAGLMVISSISG
                     MAKTSMGHLAFIAVAVMAIGNAGGRILAGMLSDRIGRRGTLFLVLLFQAVLMFAAIPV
                     TGAQNMAGIVVVVVAALIGANYGANLALFPSYCKDLWGLKSFGINYGLLFTAWGLGGF
                     ALSAVAQRLVAASQGSYRSSFITAGVLLLIGAGLTFLIKPPQPKAPA"
     gene            complement(1530..3509)
                     /gene="acs"
                     /locus_tag="PKK20_00825"
     CDS             complement(1530..3509)
                     /gene="acs"
                     /locus_tag="PKK20_00825"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNU98465.1"
                     /translation="MTEPTKTVSTDSLLQETRTFPPTPDAVQRAYINADQYKSMYDHS
                     IKDPEGFWLEQAKTLDWVRPPTKARRYLWDTAAHRIEHTWFEDGQINVSVNCLDRQLA
                     KKANKPALIWQGEPENDVRTLTYAELHREVCKCANVLKSMGVKKGDRVSIYLPMIPEL
                     PIVMLACARVGAIHSVVFGGFSAEALAGRINDSACKLLVTSNTSLRAGKAIPLKTIAD
                     EALKHTSTIEKVIVVKRNETPCNVVAGRDLWYHELMAGAAAECAAEPMHAEDPLFMLY
                     TSGSTGKPKGVVHTTAGYLLHAALSHKYIFDVHEDDVYWCTADIGWVTGHTYIVYGPL
                     CNGATSVMFEGVPTFPDAGRFWQVIQKFKATVFYTAPTAIRSLIRLGEEWPNKYDLSS
                     LRVLGSVGEPINPEAWMWYHRVIGKERCPIVDTWWQTETGAILITPLPGAHTLKPGSA
                     SRPFFGVEPVVLRDDGSECGPNEGGKLCIKKPWPGMMRTTWGDHDRFIDTYFTMYPNI
                     YFTGDGCRVDPDGDYWLMGRIDDVVNVSGHRIGTAEVESALVSHPKVAEAAVAPMPHD
                     VKGQGLYAFVTLKEGEHESEELRKELALHVRKEIGPIAVPDKIQFAPGLPKTRSGKIM
                     RRILRKIAENAVDQIGDITTLADPHVVEALVKGKQ"
     gene            complement(3548..4732)
                     /locus_tag="PKK20_00830"
     CDS             complement(3548..4732)
                     /locus_tag="PKK20_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HNU98466.1"
                     /translation="MVAFLYSPEIDGLTYPPDCPFKTQRAGLTRQRLLSFGLLGGTDC
                     VEVPTRQATRPELEAFHDPAYLDQLERAAGGDLTIEGFQMGLGGSDTPVFRDLFEYGR
                     WACGAGLDAADLLIAGKAKVAFNLFGGFHHAMPARAGGFCYLNDVVLACERLAASGRR
                     VLYLDLDAHHGDGVQEAFYRRRDVLTLSIHESGRTLYPWSGFESEIGEGPGLGYNVNV
                     PLPAGTYDEAFLGAFEAVTVPLLGAFNPDVIVLELGMDILAGDPLTHLQMTNNVVVDV
                     VEQLMKCGRPVLVAGGGGYHVENTVRGWALAWRTFCGEGDEDIYSLGLGGVMLGSSEW
                     AGGLRDRALVVTEEQRQAVEPELRSTIDRLSQTVFPHHGLGIPSAPGCGPGQDGQHER
                     PV"
     gene            5135..5440
                     /locus_tag="PKK20_00835"
     CDS             5135..5440
                     /locus_tag="PKK20_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNU98467.1"
                     /translation="MSNASRLFVGNLSYHTLENDLQDYFGQAGVVTSCNLMLDKYTGK
                     SRGFAFVEFSTAEEANKAVELFHDKEFQGRQLTVNIARPREERPERSPRRGESQMGA"
     gene            complement(5552..7528)
                     /locus_tag="PKK20_00840"
     CDS             complement(5552..7528)
                     /locus_tag="PKK20_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98468.1"
                     /translation="MKHHISRVAQLLSLAVFAASASALGPTRAATLDLDVPAAEREQK
                     LIALIKSDAQPAEKALALKQLAICGTADAVPALAGFLGSEEFSTWARIALEVIPGPAP
                     DEALRAALTTVQGRPLIGVISSIGVRRDLKAAYPLITKLRHPDAQVVSAAAIALGHIG
                     GDSAARALEISLAETSGATRSAIAEGCILCAERWLAEGQAAKAMALYDTVRKADVPQR
                     RVAEATRGAILARGSDGTALLLEQLRSPDKALYSIGLRAARELPGTQVTEALASAVKQ
                     LAPDRQWRVLLALADRHDAAVLPAVKAFAKDAPAELRVTALNVLEHVGDTSCIPLLIE
                     AATVDDRAVAQAAKASLASLHTGNVDAELLRLLPQAAGKQRQVLVEAASARRIESAIP
                     SVLRYATDADAGLRKAAFEALAALGGPKEMGAVVDLLKSATAQDRDALEQALLAISGR
                     SGAAAAPTLMPLAGHSDPSLRAVGLHALAAVGGPEALAAVIKAVDDQEPSVQDDAVRT
                     LSTWPNNWPDDNGVAEPLLALAKSGRKPMHQALGLRGYLQYLQGIKHIGNDEKLAKVR
                     ETMPLMKSSDQKQLAAAVLGAIPAPGAIDMLVGFAGDADTAEAACSALTALAVKDAPE
                     FTKDQRRKALQIVLEKSKSDGTRKRAGEALKKIQ"
     gene            complement(7573..8853)
                     /locus_tag="PKK20_00845"
     CDS             complement(7573..8853)
                     /locus_tag="PKK20_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98469.1"
                     /translation="MSAIDNQMNRRRFLKRTFPVGAMLLAPQIVAPSVLGKGGGVAPS
                     ERVVLGGIGIGGRGSYVLGCFLQEPDVRFVAVAEVKAQRRQAVKQMADTRYGNTDCAT
                     YRDLRELLARNDIDAVLIATGPNWHAAASMLAARAGKDVYCEKPCAKTIMDSLALADT
                     FRRTGRVFQAGTQRRSLPNFAYAVDLARRGRLGKLKTLHAHPAGLETKMSGWLPAETE
                     PPKEEVDWDLYLGPAAWRPFNRRLLDGFNFEKGGGMVGGGCLEWGSHCVDLCQWANDA
                     DDTSPIEYERKGDRLHARYANGVELVVRNDGWLPLGSCPVRFEGETGWVETGDNGDLV
                     ASDDALLAGKGVRISGYPADFHVRNFLDCVKTRGQPRANAEVACWSHITCNAANIALF
                     LDRKVQYDPKKNEFINDEEANRLRGEAWREPWQA"
     gene            9082..12855
                     /locus_tag="PKK20_00850"
     CDS             9082..12855
                     /locus_tag="PKK20_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNU98470.1"
                     /translation="MKPPPNSHLDLSEGAPQHRRRCQCSGSCAPAFDPVNRRTFLGRT
                     AAGAAGLLTAPAWLRAPLSAAELEAWSQSLTAPDAGAVYHSAVHTDARMHLGGIGTGN
                     IEIGSDGQFTTWQLFNTLRDGLVPFHFAVRVGNVARLLQTAGGPAAPRVPRVEMAVEY
                     PVARLRYIEPDLPVDLELEAFSPFAPLDTALSSVPVAALIFRITNRSAQPQTVSLASF
                     LQNPVGYDAIDPIDGDRHPNFGGNINEPCGEGAARGLFFRAQPGEAATLDRPIRLFIS
                     ANLAALNSPPPDRPDNLRVEPLEGLAKAAPSDAPESRVVWIEEPAADFASAQLRAARD
                     AVQEGATLVFAGATLPLVAQYATATGGQPLSPSSGRPDILFEDFEHGYDQWTLSGTAF
                     GTKPAGGTLPNQQRVSGFLGRGLVNTYLEGDGTRGKATSRAFTIERRFIRFLIGGGSH
                     ADTQLRLRIGGQVVRRASGKDNERLEPVSWDVSEHAGKSGQLEIVDEATGGWGHVNVD
                     QIEFSDHPANRATLALLEELLPVRCTEVRAVADRAPGDPNTLAFAGVTLREGAQSQAT
                     TGGLNLYVRPVGKGRVVLASGPVLRPNAAGIVSVRQAAYATLCELVGARYKPLRGQSI
                     QAPGFGSMALAALEGEVTTASAIGDWAEAWNTFARDGRWPSDTHTASSAPTPAGRTTT
                     GAVATRVTVAPNARLDVPFLLAWHYPNKYNAQGVWMGCHYATRWPDARAVLKEASASL
                     AAWRAQTDLFRRTLYESTLPAWLLDCLTANAATIRHIGVVFRIANGDIYGWEGSNGCC
                     QPTCTHVWGYEQTLARLFPDLEREMRRIDFKHQQRKDGGVNNRTDVPSPPRPTGEQPF
                     ADGHASCILKAYREALNTTDESFFQDYWPHIRRAVDYLIERDAKSHGGAPSGYLEDDQ
                     WNTYDEALHGVTTFISGYYLAALRAGEEWARRVGDTASADRFRGLFERGQARLIELCW
                     DGEYFRQHLPGYEQMPGEVGPGCMSDQLIGQWWAHQLGLGYLFPRDKVVSALKAVFKY
                     NWKSDLTGWKHAPRAFAGDNDKGLIICTWPKGGRPGHVMLYSDEVWTGIEYQVAAHMI
                     YEGLLTEGLAIVKGARERYDGIPRRPIMRNPWNEIECGGHYARAMSSWSLLLALSGWA
                     YDGPARTLRLDPRYRPEQIRCLFAGPEGWGSFAQRIEEGRQRDELNVRWGRLAVKQIE
                     VALRPGAAPVAVTVRVGGRDVACQSAAAGGHAQVRFDQDIVIEAGQSLVLEFA"
     gene            12927..13406
                     /gene="ispF"
                     /locus_tag="PKK20_00855"
     CDS             12927..13406
                     /gene="ispF"
                     /locus_tag="PKK20_00855"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNU98471.1"
                     /translation="MNHVGIGYDVHRLVEGRKLLLGGVDLRHHKGLEGHSDADVLLHA
                     ICDAIFGALGEGDIGQLFPNTDPRWHNAPSKIFLHEAARLVNLRGGRIVNIDSTVIAE
                     QPMLAPHLHAMKSNISHILELHPSRLSIKATTNEGLGFLGRDEGIAALAVVSLELPQ"
     gene            13474..13752
                     /locus_tag="PKK20_00860"
     CDS             13474..13752
                     /locus_tag="PKK20_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98472.1"
                     /translation="MSAKTEIGWRRVDEQGEHWQVYAHRVGGAWRFFERHRRYEDWRP
                     VANPSLADWLRLLEAVERRMPRRRMPAAEVERIRQRIRELFPTASREQ"
     gene            13749..15131
                     /locus_tag="PKK20_00865"
     CDS             13749..15131
                     /locus_tag="PKK20_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNU98473.1"
                     /translation="MKTRVLVVDDDRAVCQMLHAVLAEAGHEPSVALDAASLFRLPES
                     PAPHVIILDWQLPDGDGIALLPQVKRHWPRSEVIILTGYGTFDAAVEATKLGAFHFIG
                     KPVDIPALLLLIRRAAEHMQLVAQTQVLRDALSSLGGGASPIFRSPAMQQVLRLVERV
                     APSDAAILLSGESGTGKEVIADLIHALSRRSSGPLVKVNCAALPRELIESELFGAVKG
                     AYTGAVADRDGLFRQAEGGSLFLDEISEMPLDTQAKLLRVLQEKRVRPVGGRTAYATD
                     CRIVAATNRPLPTLIEERKLREDLYYRIAAVRIELPPLRERHEDIVPLARAFLRRFAS
                     QAARPVTDFSPDAIDCLCQHAWPGNVRELENEVQRAVLLCEAAVIQPTDLSIVVAGHS
                     APGARVQAALTPLEVVERDTIVRVLREAQGNKLAAARRLGIGRQTLYNKIAQYGLGPD
                     APLRPGRENK"
     gene            15334..16443
                     /gene="dnaN"
                     /locus_tag="PKK20_00870"
     CDS             15334..16443
                     /gene="dnaN"
                     /locus_tag="PKK20_00870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNU98474.1"
                     /translation="MKLTIAREQLINGLQAVQNVVGSRTTLPILSNVLLQAEQGRLGL
                     TATDLDVSIACGVEAKVEKVGVTTLPARRLFSIVRELGTPEIELEVDEKNVCSLTAGA
                     SFFKVNGLPAEDFPPRPGFADSKRVALPQDKVKGMLRRTAFAVSNDEARYVLNGLFFS
                     LREHKVTLVATDGRRLALTDEEVDIGAENQTEFIVPAKTVAELSRLLQDKGEVEISVG
                     DNQVAFDMRGEGLLNILAISKLVEGTYPNYQQVIPRESKERIPLVREELVQALRRAEL
                     MTSEKSNSVKLSFTRNTLAITANAPEIGEARETLAINYKGPDLAVAFNPSFLLDALKA
                     LDNDEVFLELSDELSPGVLKVNGPFLYVIMPMRMN"
     gene            16524..17384
                     /gene="folP"
                     /locus_tag="PKK20_00875"
     CDS             16524..17384
                     /gene="folP"
                     /locus_tag="PKK20_00875"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNU98475.1"
                     /translation="MRWSCRRFEFVFPRPALVMGIVNVTPDSFSDGGRYFDPGAAIAH
                     ARELVAEGADFLDVGGESMRPNATPVSEAEELRRILPVIEELARQVTVPLSVDTRKSG
                     VARAALAAGASLVNDVEARRSDDALWRVVADAQAGYVCMHMQGTPATMQQNPTYTDVV
                     REVGEFFTDRLARLARCGVGPDQVALDVGIGFGKDLDHNLDLLAGLESYKGFHRPLLL
                     GVSRKSFLGRLLGVDLDARLPAALACTAWAANAGVQVFRTHDVAATCQALRVIEALRA
                     RAATAACPGH"
     gene            17384..18223
                     /gene="cdaA"
                     /locus_tag="PKK20_00880"
     CDS             17384..18223
                     /gene="cdaA"
                     /locus_tag="PKK20_00880"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNU98476.1"
                     /translation="MDWLPKDWRAFVEILILAIGIYYAVMLVRGTRGAPVVTGFLLVL
                     MVLTVLARALDLEVFEGLLGNFFAFFAVGVIVIFQPELRRVLAELGSLPFFASTREQR
                     ENIEVIIKTAERLADVRIGMLLAIEQSIQLQEVVESGIVVDAEATPEMLETIFFPNNA
                     IHDGGVILCGNRIMNAACIFPLTQRQDLSKSLGTRHRAAIGLSEETDAVVVVVSEETG
                     AVSYAYKGQLIRGVTAEELRSFLTSVLVRIPRSRTVGEWLRTLVTREPAAGVAKPARA
                     KTD"
     gene            18228..18647
                     /locus_tag="PKK20_00885"
     CDS             18228..18647
                     /locus_tag="PKK20_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNU98477.1"
                     /translation="MRLPRILTHNLRWKMVALLSAAVVWMTLKSGTPSRNQPRGSRVL
                     RQLPITIMTSTEDRRAVRVDPNTVDVQVSGAPEVVDRLEARDIRVFVDLAPIEDERQF
                     RLRVEVHTPFGVILEKVTPSEVDGQSLPPNPTPQSRQ"
     gene            18802..19167
                     /locus_tag="PKK20_00890"
     CDS             18802..19167
                     /locus_tag="PKK20_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98478.1"
                     /translation="MNITNITRLGLIAFVSLLAGCATSNLPKGARLVGGGLSVEYTAP
                     RDGTAILVERTSGRIVATESLGEGDSFDFNPDVENYDEVLFRMFGDIHAASDGSTMVV
                     IPTNALFQLYFVPEKTRKE"
     gene            19462..19653
                     /locus_tag="PKK20_00895"
     CDS             19462..19653
                     /locus_tag="PKK20_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98479.1"
                     /translation="MAWVHPEQFRRCVVRYRADYKVLTSRRSSRQTTLSPLAVVVLAI
                     KFTIVWLAFPHWLQFPDRV"
BASE COUNT         3442 a         6390 c         6302 g         3532 t
ORIGIN      
        1 ttcaacgaag cggttcgtta aaagttggac gttgaacgtt gcgcgttgga cgttcaggtt
       61 accccttttc ttaattatca acggcttgcg caggcgattc atggaaagcg agttcacatc
      121 ggccgcaggc tcaggcgggc gccttgggct ggggcggttt gatcaagaac gtcagcccgg
      181 ccccgatgag caacaacacc ccagccgtga tgaacgaaga tcggtagctg ccctggctcg
      241 ccgcgaccag gcgctgggcc acagcgctca gggcgaatcc acccagaccc cacgctgtaa
      301 acagcagacc gtagttgatc ccgaaactct tcaggcccca gaggtctttg cagtaggacg
      361 ggaacagggc gaggttggcg ccatagttcg ccccgatcaa cgcggccacg acaacgacta
      421 caatcccggc catattctgg gcgcccgtca ccggaatggc ggcaaacatc aggaccgcct
      481 gaaacagcag caccagaaaa agcgttccac gacgcccgat ccggtccgag agcattccgg
      541 ccaggatgcg cccgccggcg ttgccgatgg ccatgaccgc cactgcgatg aaggcgaggt
      601 ggcccatgct ggtcttggcc atgccactga tgctgctgat gaccatgagg ccggcgccgg
      661 cgccgatgaa atagatgatc caaagcaggt agaacagcgg ctgacgcagc atctcgcccg
      721 gtgttacgtt cgaggcagcc ggcttgggcg ccgccgatcc ggctcccgca ggcgtgccgc
      781 cggccacata gcccggtggc ggattcacca gcaactgcgc cagggcgcaa acgaggatga
      841 caaaggcgat gccgaagatg agcatcgact tctgcagggc gaaatgggcg agcaggtatt
      901 gggaggtggg cgcgaggtag acgggcgcca ggccgaagcc tgccacgacc aggccggcaa
      961 ccaaaccggt cttcgatgga ggaaaccact tgagggcagg cggagtggcc gacgaatagc
     1021 caaagccgat gccgatgccg accagcaggc cgaacccgag gagccaaatc gcgtagttgg
     1081 tggtggtggc gatcagcacc atgcccgtcc cgacgagcag gccgccgatc gaggctgtca
     1141 gccgcgggcc gaacttgtcc tggcagcggc ctgccaggat catcgtgaag gcaaacgcca
     1201 ggcaacagag cgcgtacgga tcgttgagat ggtccccttc ccagccaaac tccttctcga
     1261 tggcgccttt gaacatgctc tacgtgtaga gcacgccgag cgcaaggttg attccgagtc
     1321 cggcaaacgt gaccgtccag ccgcgatgag caacgggcgc agtggaggga ttggattcag
     1381 ttggcatacg gtatgggctg agttgagtgt tggctgaagc gaaagggctg gagttcctgg
     1441 gggaagatcg agcgggcgga tggcgccgcc ggggcaccgg accccggcgg cacgaaacac
     1501 gcggcggcac tggggccgcg ccggggcatt tactgttttc ctttgaccag cgcctcgacc
     1561 acatgcgggt cggccagcgt ggtgatgtcg ccgatctgat cgacggcgtt ctcggcgatc
     1621 ttgcgcagga tgcgccgcat gatcttgccc gatcgcgtct tgggcagccc cggggcgaac
     1681 tggatcttgt cgggcaccgc gatgggcccg atttccttgc ggacatgcag ggccagttcc
     1741 ttgcggagtt cctcgctctc gtgctcacct tccttgagcg tgacgaaggc gtagagcccc
     1801 tggcccttga cgtcgtgcgg catcggggcg acggcggcct cggccacctt cggatgactg
     1861 acaagcgcgc tctcgacctc ggccgtaccg atgcggtgtc cggacacatt cacgacatca
     1921 tcgatgcggc ccatcaacca gtaatcgcca tccggatcca cgcggcagcc gtcgcctgtg
     1981 aagtagatgt tcggatacat tgtgaagtag gtgtcgatga accggtcgtg atcgccccag
     2041 gtcgtccgca tcatgccggg ccacggcttc ttgatgcaga gcttgccccc ttcgttcgga
     2101 ccgcattccg acccgtcgtc gcgcaggacg accggctcga caccgaagaa cgggcggctg
     2161 gcactgcccg gcttgagggt gtgcgccccc ggcagcgggg tgatcagaat ggcgcccgtc
     2221 tcggtctgcc accaggtgtc cacgatcggg cagcgctcct tgccgatgac ccggtgatac
     2281 cacatccacg cctcgggatt gatcggctcg ccgaccgagc ccagcacgcg aaggctgctc
     2341 agatcgtact tgttgggcca ctcctcgccg aggcggatca gggaccggat ggcggtcggc
     2401 gcggtgtaga acaccgtggc cttgaacttc tggatcacct gccagaagcg gcccgcatcg
     2461 gggaaggtcg ggacgccttc gaacatgacg ctcgtggccc cgttgcacag ggggccatag
     2521 acgatgtagg tgtgacccgt cacccagccg atatcggcgg tgcaccagta aacatcgtcc
     2581 tcgtggacgt cgaagatata cttgtgcgac agggcggcgt gcaggaggta gccggccgtg
     2641 gtgtgcacca cgcccttggg cttgccggtc gaccccgatg tgtagagcat gaacaaggga
     2701 tcctcggcgt gcatcggctc cgctgcgcac tccgccgcgg cccctgccat caactcgtgg
     2761 taccagagat cgcgtccggc gacgacgttg cagggcgttt cgttgcgctt gaccacgatg
     2821 accttctcga tggtgctcgt gtgcttcaga gcttcgtccg cgatggtctt gagcgggatc
     2881 gccttgcccg cgcgcagcga ggtgttcgag gtcaccagca gcttgcacgc ggaatcattg
     2941 atgcgcccgg ccagcgcctc ggcgctgaat ccgccgaaca cgaccgaatg aatcgccccg
     3001 acccgcgcgc aggcgagcat gacgatcggc agctcgggaa tcatgggcag gtagatcgag
     3061 acccgatcgc ccttcttcac gcccatcgac ttgagcacgt tggcgcattt gcagacctcg
     3121 cggtgcagct cggcgtacgt gagcgtccgg acatcgttct ccggttcgcc ctgccagatc
     3181 agagccggct tgttcgcctt cttcgccaac tgccggtcga ggcaattgac gctcacgttg
     3241 atctggccgt cctcgaacca ggtgtgctcg atccgatgcg ccgctgtatc ccacaggtaa
     3301 cgcctggcct tggtgggagg ccgaacccag tccagcgtct tggcctgttc gagccagaat
     3361 ccctccggat ccttgatcga gtggtcgtac atggatttgt attgatcggc attgatatag
     3421 gctcgctgga ccgcgtccgg cgtcggcggg aacgtgcggg tctcttgcag cagggaatcg
     3481 gtcgaaactg tcttggtggg ttcggtcatg tctgtctatc ctttcctagt tcaatggtgc
     3541 gtgtttgcta aactggtcgc tcgtgttggc cgtcctggcc tgggccacag cccggagcgg
     3601 atgggatgcc caatccgtgg tgcgggaaca cggtttgaga aagcctgtcg atggtgctcc
     3661 gcagttcggg ttcaaccgcc tgacgctgct cctcggtcac aaccagcgcc cggtcacgca
     3721 ggccacccgc ccactcgctg ctgccaagca tcacccctcc cagcccaagg ctgtaaatgt
     3781 cctcatcccc ttcaccgcaa aacgtccgcc acgccagcgc ccagcctcgc acggtgttct
     3841 cgacgtggta tccgccgccg cccgccacaa gcaccggacg accgcacttc atgagctgct
     3901 cgaccacatc cacaaccaca ttgttggtca tctgaaggtg cgtgagcgga tcgccggcca
     3961 ggatgtccat gcccagttcg agcacaatga catcgggatt gaaggcgccc agaaggggga
     4021 cagtcaccgc ttcgaaggcg cccagaaagg cctcgtcgta cgtcccggca ggcaatggca
     4081 cattcacatt gtagcccagg ccggggcctt cgccgatttc cgactcgaag ccgctccacg
     4141 gatagagcgt gcgcccgctc tcgtggatcg agagcgtgag aacgtcgcgg cgcctataaa
     4201 aggcctcctg cacgccgtcg ccatggtggg cgtccaaatc cagatacagc acgcgccgac
     4261 cggatgccgc caggcgttcg caggcgagga cgacgtcgtt gagatagcag aaaccgcccg
     4321 ctcgagcggg catggcatgg tgaaaccctc cgaacaggtt gaaggcgact ttcgccttgc
     4381 cggcgatcag aagatccgcc gcatcgagcc cggccccgca cgcccagcgc ccgtactcga
     4441 acaaatcccg aaacaccggc gtgtccgatc cgcccagccc catttggaac ccctcgatcg
     4501 tcaggtcgcc gcccgccgcc cgctcgagct gatccaaata ggcggggtcg tgaaaggctt
     4561 cgagttcggg gcgcgtcgcc tggcgcgtcg ggacctcgac gcagtcggtc ccccccagga
     4621 ggccgaagga gagcaggcgc tggcgggtca gcccggcgcg ttgcgtcttg aagggacagt
     4681 ccggcggata ggtcagaccg tcgatctcgg gtgaataaag gaaggccacc atccggtcat
     4741 gcatagggct catcggggcg gctgggacct cggccccggc ggttgggatt gcacgcgggc
     4801 cgaaggcaaa cggagcaccc cctcgcagtc ggctgaaggc gcgccgaggc tgcgatggcg
     4861 tacagcggtc cactggtgac aaacccggat cgacagtggc atctatggac cgtaagaagt
     4921 ggtgattggg gcgggcttca ccctttttgc caattaatca accttttttg agttgcgatt
     4981 ctctggttga cgccccgttt gattttgcta cggtgcgcct tgcgcaaaga attagtcgtg
     5041 tcggtgtgac agatgtccgc atacaatcca agtgggcttc ggtgatcctg cgagtgcagt
     5101 cccctctgtt ttctttgagc ggtttatacc cattatgagc aacgcgagca gactgttcgt
     5161 cggtaatctc tcctatcata ctctcgaaaa tgacctgcag gactatttcg gacaggccgg
     5221 tgtggtcacc tcctgcaacc tgatgctgga caagtacacc ggcaagtcac gcggcttcgc
     5281 attcgtcgag ttctcgaccg cggaggaggc taacaaggcc gtcgaactct ttcacgacaa
     5341 ggagtttcag ggccgccaac tgactgtgaa catcgcccgg ccgcgcgaag agcgccctga
     5401 gcggtcgccg cgtcggggcg aaagccagat gggcgcctga tcggctcact tccccgccct
     5461 ttcgacggcg ggttggcgcc agaaagaaaa accgattcga tgccgccaac gcggcatcga
     5521 atcggtttgt gaatcggagg tctggcgctg tctactggat cttcttcagc gcttcaccgg
     5581 cgcgcttgcg cgtgccgtcg ctcttggact tctcgaggac gatctgcaag gccttgcggc
     5641 gctggtcctt ggtgaactcg ggagcgtctt tcaccgccag ggcggtcaac gcggagcacg
     5701 cggcctcggc cgtgtccgca tcgcccgcga atcccaccag catgtcgatc gctcccggtg
     5761 ccgggatggc cccgagcaca gccgccgcca gttgtttctg gtcagacgat ttcatcagcg
     5821 gcatcgtctc cctaaccttc gccagcttct cgtcattccc gatgtgtttg atcccctgga
     5881 ggtattggag atagccgcgg agcccgagcg cctggtgcat cggtttgcgc cccgacttcg
     5941 ccagtgccaa caacggctcg gcaacaccgt tgtcgtcagg ccagttgttc ggccacgtcg
     6001 acagggtccg cacagcgtcg tcctgcacgg atggctcctg gtcgtcaacg gcctttatca
     6061 ccgcggccag ggcttcggga ccgccgacgg ccgcgagggc atggagccct acggcccgga
     6121 gcgaggggtc gctgtggccg gccagaggca tgagcgtcgg cgccgcggcc gctccgctcc
     6181 gcccgctgat cgccagcagc gcttgctcga gggcgtcgcg atcctgcgcg gttgcggact
     6241 tgagcaagtc aactacggcg cccatttctt tcgggccgcc cagggcagcg agcgcctcga
     6301 acgctgcctt gcgcagaccc gcatcggcgt cggtggcata gcgcaacacg gacgggatcg
     6361 ccgactcgat gcgccgggcg gacgcggcct cgacgaggac ctggcgttgt ttgccggcgg
     6421 cttgcggcag gaggcgcagc aactcggcat cgacattgcc ggtgtgaagg ctggccagag
     6481 aggctttggc ggcttgggca acagcccggt cgtcaaccgt ggccgcttcg atgagcagcg
     6541 ggatgcacga ggtgtcgccg acgtgttcca gcacgttcag ggcggtcacc cgcagttcgg
     6601 cgggggcgtc cttggcgaag gctttgaccg ccggcagcac ggcagcgtcg tggcgatcgg
     6661 ccaacgcgag cagcacccgc cactgcctgt ccggagcgag ttgtttcacg gcggatgcca
     6721 gggcttcggt gacctgagtc cccggcagtt cgcgcgcggc gcgcaggccg atactgtaga
     6781 gggccttgtc gggcgatcgc agttgttcga gcagaagcgc cgtgccgtcc gaaccgcggg
     6841 ccagaatcgc gccgcgcgtt gcctcggcca cccgccgttg cggcacatcc gccttgcgca
     6901 ctgtgtcata gagcgccatg gctttggccg cctgaccctc ggcgagccac cgttcggcac
     6961 acaggataca gccttcagca atggccgagc gcgtggcgcc gctggtctcg gccagagaga
     7021 tttctagggc ccgggccgcg gagtcgccgc caatatgtcc caaggcgatg gccgctgccg
     7081 acacgacctg ggcgtctggg tgtctcagtt tggtgatgag tgggtaggcg gccttaagat
     7141 cgcgccggac gccgatcgag ctgatgacgc cgatcagggg ccggccctgg acggtggtca
     7201 gagcggcgcg caaggcttca tcgggcgccg ggcctggaat gacttcgagg gcaatccggg
     7261 cccaggtcga gaactcctcg cttccgagaa agccagccag ggccggaacg gcgtcggcgg
     7321 tgccgcaaat ggccaactgc ttgagggcga gagccttctc ggcaggctgc gcgtcggact
     7381 tgatcagcgc gatgagcttc tgctcgcgct cggcggcagg cacatcgagg tccaacgtgg
     7441 ccgcgcgcgt cgggccaagg gcgctggcgc tggcggcaaa cacggcgaga ctgaggagct
     7501 gcgcgactcg ggagatgtga tgtttcatag tcttgtgaaa tggcattgtc gattcggggt
     7561 tggtgcgctg tttcaggctt gccacggttc gcgccacgcc tccccgcgca ggcggttggc
     7621 ctcctcgtcg ttgatgaact cgttcttctt gggatcgtat tggaccttcc gatccaggaa
     7681 gagcgcgata ttggcggcgt tgcatgtgat gtgcgaccag caggccacct cggcgttggc
     7741 gcggggttga ccgcgcgtct tgacgcaatc gaggaagttg cgcacatgga aatcggccgg
     7801 gtagccggag attcggaccc ctttgccggc gagcagggcg tcgtcgctgg ccaccagatc
     7861 gccgttgtcg cctgtctcca cccacccggt ttcgccctcg aaacggacgg ggcaggagcc
     7921 cagcggcagc cagccatcgt tgcgcaccac caactcgacc ccgttggcat agcgggcgtg
     7981 gagccggtcg cccttgcgct cgtattcgat cggactggtg tcgtcggcgt cgttggccca
     8041 ttggcacaga tcaacgcagt gcgaacccca ctcgagacag ccgccgccga ccatcccgcc
     8101 gcccttctcg aagttgaagc cgtcgaggag ccggcgattg aagggccgcc acgccgcggg
     8161 cccgaggtac aaatcccaat cgacttcctc cttgggaggt tcggtctcgg ccggaagcca
     8221 tccgctcatc ttcgtctcga gtccggcagg gtgcgcgtgg agcgtcttga gcttgcccag
     8281 cctgccgcgc cgggccaggt ccaccgcata cgcgaaattc ggcaggcttc gccgctgggt
     8341 gcccgcctga aacacgcggc ccgtccgccg gaacgtgtcc gccagggcga gactgtccat
     8401 gatcgtcttg gcacacggct tctcgcagta gacgtctttg ccggcccgcg cggccagcat
     8461 cgaggccgcg gcgtgccagt tggggcccgt ggcgatcagg accgcgtcga tgtcgttccg
     8521 ggccagcaat tccctgagat cgcgataggt ggcgcaatcg gtgttgccgt accgcgtatc
     8581 agccatctgc ttgaccgcct ggcgccgctg ggccttcacc tcggcgaccg ccacgaaacg
     8641 cacatccggt tcctgcagga agcagcccag gacgtaggag ccgcgtccgc cgatcccgat
     8701 gccgccgagc accacgcgct cgctgggggc gacgcccccg cccttgccca ggaccgatgg
     8761 agccacgatt tgaggcgcca acagcatggc gccgaccggg aatgttcgtt tgagaaaccg
     8821 ccgcctgttc atttgattgt cgattgcact catgatttgt ctccgctcag ggctgccctc
     8881 gcgacgtctg gtcgacacgt ctctccaccg tggttggttt cgcggcggcc cgttgcgccg
     8941 acatggtgag acgggcgatc atcagggtca agcctcgcgg cgcggcaatc ggactgctca
     9001 tccacgacaa ggaagcgaca gcgatgattc gggcgtgacg ccgggcaccc aatcctctaa
     9061 cacatacgca cactgcaaat catgaaacct ccgcccaatt cccatctcga cctttccgag
     9121 ggcgctcctc aacacaggcg ccgatgccaa tgctccgggt cctgcgcccc ggcgttcgat
     9181 cccgtcaatc gccggacgtt cctcgggcgc acggcggccg gggcggcagg cctcctgacc
     9241 gcgccggcct ggctgcgcgc gccgctgtct gcggccgaac tcgaggcctg gagccaaagc
     9301 ctgaccgcgc cggatgccgg cgcggtctac cactccgccg tccacaccga cgcgcggatg
     9361 cacctgggcg ggatcggcac cgggaacatc gagatcggct cggacggcca attcaccacg
     9421 tggcagttgt tcaacaccct gcgcgacggg ctcgttccct tccatttcgc cgtccgtgtc
     9481 ggcaacgtgg cccggctgct ccagaccgcc ggcggccccg cggccccgcg cgtgccgcgc
     9541 gtcgagatgg cggtcgagta tcccgtcgcc cggcttcgct acatcgagcc cgacctgccg
     9601 gtcgacctcg aactcgaggc gttcagcccc tttgcgcctc tcgacaccgc cctctcatcg
     9661 gttccggtgg ccgccttgat cttccgcatc accaaccgct ccgcccagcc gcagacggtg
     9721 tcgctcgcct cgttcctcca aaaccccgtc ggttacgatg ccatcgatcc gatcgacggc
     9781 gaccggcatc ccaacttcgg cggcaacatc aacgaaccgt gcggtgaagg ggctgccaga
     9841 gggctcttct tccgggctca gcccggtgaa gcggcgacgc tggaccgtcc gatccggctg
     9901 ttcatcagcg cgaatctcgc ggccctgaac agcccgccgc ccgatcgccc ggacaacctg
     9961 cgggtcgaac cgctcgaagg cctggccaaa gcggcgccat cggatgcgcc cgaatcgagg
    10021 gtcgtctgga tcgaggagcc cgccgccgac ttcgcctccg cgcaactccg ggccgcgcgc
    10081 gacgccgtgc aggagggagc gacgcttgtc tttgcgggcg ccacactgcc gctggtagcc
    10141 cagtatgcaa ccgccacagg cgggcaaccg ctgagcccca gctcgggccg ccccgacatc
    10201 ctgttcgaag acttcgagca cggctacgac caatggacac tcagcgggac ggcgtttggg
    10261 acaaaacccg caggcggcac gctgcccaat caacagcggg tctcgggttt cttgggacgc
    10321 ggcctggtca acacctatct cgagggcgac ggcacgcgag gcaaggcgac aagccgcgcg
    10381 ttcaccatcg aacgccgttt catccgcttc ctcatcgggg gcgggtccca cgccgacacc
    10441 caattgcgcc tgcgcatcgg cggccaggtc gtgcggcggg cttcgggcaa agacaacgaa
    10501 cgcctcgaac cggtgtcgtg ggacgtgagc gagcatgcgg gcaagtcggg tcagctcgag
    10561 atcgtcgacg aggccaccgg cggctggggc cacgtgaatg tggaccagat cgagttctcg
    10621 gatcacccgg ccaatcgggc caccctggcc ttgctcgagg aactgctgcc cgtccgctgc
    10681 accgaggtcc gggccgtcgc ggaccgcgcg cccggggacc ccaacaccct cgcgttcgcc
    10741 ggggtcaccc tgcgcgaggg cgcccagtcc caggccacga cgggcggact caatctctac
    10801 gtgcgcccgg ttggaaaggg gcgtgtcgtt cttgcatcgg gtccggtcct gcgtccgaac
    10861 gccgccggga tcgtttcggt ccggcaggcc gcatacgcca ccctctgtga actggtcggg
    10921 gccaggtaca agccgctgcg tggccagtcg atccaggcgc ccggattcgg ctcgatggcc
    10981 cttgctgcgc tcgaaggcga ggtgacgacc gcgtcggcca tcggagattg ggctgaggcc
    11041 tggaacacct tcgccaggga tgggcgctgg ccgtcggaca cccacaccgc ctcgagcgcg
    11101 cccacgcccg cggggcgaac aaccaccggc gccgtggcca cccgcgtgac cgtcgcgccc
    11161 aacgcgcgcc tcgatgtgcc gttcctcctg gcctggcatt acccgaacaa gtacaacgcg
    11221 cagggggtct ggatggggtg tcactacgcc acccgatggc ccgacgcgcg cgccgttctg
    11281 aaggaggcgt cggccagcct ggccgcctgg cgcgcccaaa ccgacctgtt ccgacgcacc
    11341 ctttacgaga gcacgctgcc ggcgtggctc ctggactgcc tgacggcaaa cgccgccacc
    11401 atccggcaca tcggcgtcgt gttccgcatt gccaacggcg acatctacgg ctgggaaggc
    11461 tcgaacggct gttgtcagcc gacctgcacc cacgtctggg gctacgaaca gaccctcgcg
    11521 cggctgttcc ccgatctcga acgcgagatg cgccgcatcg atttcaaaca ccagcagcgc
    11581 aaggacggcg gcgtgaacaa tcgcactgac gtcccgtccc cgccccgccc gacgggcgaa
    11641 cagcctttcg ccgacggcca tgcgagttgc attctcaagg cctatcgcga agccctgaac
    11701 actaccgacg aatcgttctt ccaggactac tggccccaca tccgccgggc cgtcgattac
    11761 ctcatcgagc gcgacgctaa gtcccacggg ggcgcgccgt ccggctatct tgaagacgac
    11821 caatggaaca cctacgacga agcgctgcac ggtgtcacca cgttcatcag cggctattac
    11881 ctggcggcct tgcgcgccgg cgaggaatgg gcgcgccgcg tcggcgacac cgccagtgcc
    11941 gaccgcttcc gcgggctctt cgagcggggt caagccaggc tgatcgagct ctgctgggac
    12001 ggcgagtact tccgccaaca cctgcccggc tacgagcaga tgcccggcga ggtcggcccc
    12061 ggctgcatga gtgaccagct catcggccaa tggtgggcgc accaactcgg cttgggatat
    12121 ctgttcccgc gggacaaggt cgtctcggcc ctcaaggcgg tcttcaagta caattggaaa
    12181 tcggatctca ctggctggaa acacgcgccg cgcgcctttg ccggcgacaa cgacaagggc
    12241 ctgatcatct gcacatggcc aaaaggcgga cgccccggcc atgtcatgct ctactcggac
    12301 gaagtgtgga ccggcatcga gtaccaggtc gccgcccaca tgatctacga agggctcctc
    12361 accgaaggcc tcgccatcgt caagggcgcc cgcgagcgat acgacggcat cccgcgccgg
    12421 ccgatcatgc gcaacccgtg gaacgaaatc gagtgcggcg gccattacgc ccgcgcgatg
    12481 tcgtcctggt cgctgctgct ggcgctctcg ggctgggcgt atgacggccc ggcccgcacg
    12541 ctgcgcctcg acccccggta ccgtccggag caaatccggt gtctcttcgc aggaccggag
    12601 ggatggggca gctttgcgca gaggatcgaa gaggggcggc agcgcgacga gctgaatgtc
    12661 cgctggggac ggctggctgt gaaacagatc gaggtcgctc tgcggcccgg ggctgcgccc
    12721 gtcgccgtca cggtccgcgt cggcggccgc gacgtcgcgt gccagtctgc cgccgcgggc
    12781 ggacatgccc aggttcgctt cgaccaggac attgtgattg aagccgggca gagcttggtc
    12841 ttggaattcg cctgagctgc gcccggcgtc cagtcgagcg attctcctgg tttacaatcg
    12901 gtgggcgatc cgtttatgct ggcgccatga accacgtggg gatcggatat gatgtgcacc
    12961 gcctagtcga ggggcgcaag ctcctattgg gcggcgtcga tctgcgtcac cacaagggac
    13021 tcgagggcca ctcggatgcg gacgtgctgc tccatgcgat ttgtgacgcc atcttcgggg
    13081 ccctgggcga gggggatatt ggccagctct tcccgaacac cgatccacgc tggcacaatg
    13141 cccccagcaa aatcttcctg cacgaagcgg cccgcctggt caatttacgt gggggccgca
    13201 tcgtcaatat cgactcgacg gtcatcgccg agcagccgat gctggcgcct catctgcacg
    13261 ccatgaaatc gaatatctcg cacatcctcg aactccatcc gagccgcctc tcgatcaagg
    13321 cgaccaccaa tgaggggctt gggttcctgg gacgcgacga aggcatcgct gctctggctg
    13381 tggtcagcct cgagctgccc cagtaaccag gcgggatccc gggatcgcgt cggcatctgc
    13441 cgcttcgagt cgggacaccc gaacaagcaa cttgtgtcag ccaagacgga aatcggatgg
    13501 cgccgcgtcg acgagcaggg cgagcattgg caggtttatg cccatcgcgt cggcggcgcg
    13561 tggcgcttct tcgaacgcca ccgccgctat gaggactggc gtccagtcgc caacccgtcc
    13621 ttggcggatt ggctccgatt gctcgaggcc gtcgagcgcc gcatgccgcg ccgccgcatg
    13681 ccggcggccg aggtggagcg gatccggcag cgcatccgcg agcttttccc gacggcttcc
    13741 agggaacaat gaagacacgc gtcctggtgg tcgatgacga tcgggctgtg tgccaaatgc
    13801 tgcatgcggt tctggcggag gcgggccacg agccgagcgt cgcgcttgat gccgccagcc
    13861 tgtttcgtct gccggagtcg cccgctccgc atgtgattat tctggattgg cagttgcctg
    13921 acggggatgg gatcgcgctc ctcccgcagg tcaagcgcca ctggccacgc tcggaagtca
    13981 tcatcctgac cggctatggc accttcgacg ccgctgtcga ggcaacgaag ctcggcgcct
    14041 tccacttcat cggcaagcca gtggatatcc ccgcgttgct gttgctcatc cggcgtgcgg
    14101 ccgagcacat gcagctggtc gcccagacac aggtcctgcg cgatgccctg tccagcctcg
    14161 gcggcggggc ttccccgatc tttcgcagcc cggcgatgca gcaggtgctg cgtctggtcg
    14221 agcgcgttgc gcccagtgac gccgccatcc tcctgtccgg cgagagcgga accggcaagg
    14281 aagtgatcgc cgatctcatc catgccctga gccggcgcag ctcgggcccg ctcgtgaagg
    14341 tcaactgcgc cgcattgccg cgcgagctga tcgagagcga actctttggc gccgtcaaag
    14401 gagcctacac cggcgcggtc gcggaccgcg acggcctgtt ccgccaggcc gagggcggat
    14461 cgctcttcct cgatgaaatc agcgagatgc cgctcgatac gcaggccaaa ctcctgcgtg
    14521 tgctgcagga aaagcgcgtt cggccggtcg gaggccggac ggcctatgcg accgactgcc
    14581 ggatagtcgc ggcgaccaat cgcccgctgc caaccctgat cgaggagcgc aagctgcgcg
    14641 aagacctcta ctatcgcatc gcagcggtgc gcatcgagct gcctccattg cgggagcgcc
    14701 acgaggacat cgtccccctg gcacgggcct ttctccgccg cttcgcgtct caggcggcgc
    14761 gccccgtgac cgacttctcg cccgatgcaa tcgattgtct gtgccagcac gcatggccgg
    14821 gcaatgtccg cgaactcgag aacgaggtcc aacgggccgt cctcctgtgc gaagcggcag
    14881 tgattcagcc gacggacctg tccattgtgg tcgccggtca ttcggcgccg ggcgctcgag
    14941 tccaggccgc cctgactccg ctcgaggtcg tcgaacgcga cacaatcgtg cgcgtcttga
    15001 gggaagccca gggaaacaaa ctcgctgcag ccaggcgcct gggcatcggc cgccaaacgc
    15061 tctataacaa gatcgcgcag tatggcttgg gcccagacgc cccgctacgg cccggccggg
    15121 aaaacaagtg agcgaacgga ctgtgaacaa ccgaccgata agaggctgcg aactcgatca
    15181 caccggtgtt gtttgggggg cagccacagc ctgtcctcaa gggccgggca acgcaactcc
    15241 ttgccgtgac cttgacagcg tgaactgttt caattattca cacaccataa taagagtctt
    15301 ttttgacggt ttgcagcatc atcctatgcg cccatgaaac tcacaatcgc cagagagcag
    15361 cttatcaatg gtcttcaggc ggtgcagaat gtggtcggga gccggacgac cctgcccatt
    15421 ctgtcgaatg ttctgctcca ggccgaacag gggcgcctcg gtttgacagc cacggacctc
    15481 gacgtgtcga tcgcctgcgg tgtcgaagcc aaggtcgaga aggtcggagt gaccactttg
    15541 cccgcccgcc gtcttttcag catcgtccgt gaactgggga cgccggaaat cgaactcgag
    15601 gttgacgaga agaacgtctg cagcctgacc gccggagcct ccttcttcaa ggtcaatggg
    15661 ctgcccgcgg aagattttcc cccgcggccg gggtttgccg acagcaagcg ggtcgcgctc
    15721 ccccaggaca aggtcaaggg catgctacga cgcacggcct ttgccgtctc gaatgacgag
    15781 gcgcgctatg tgttgaacgg cctgttcttc agcctgcggg agcataaggt gactctggtg
    15841 gccacggatg gacgccgctt agcgctgacg gacgaggagg tggacattgg ggctgagaac
    15901 cagaccgagt tcatcgtacc ggccaagacg gtcgccgaac tcagccgcct gctgcaggac
    15961 aaaggcgagg tcgaaatcag cgtcggcgac aatcaggtcg ccttcgacat gcgcggcgag
    16021 ggtttactca acatcctcgc gatttcgaag cttgtcgagg ggacctatcc gaattaccag
    16081 caggtcattc ctcgggaatc caaagagcgc atcccgttgg tgcgcgagga actcgtccag
    16141 gccctgcggc gcgccgaact gatgaccagc gagaagtcca actcggtcaa gctctccttc
    16201 acccgcaaca ccctagccat taccgccaat gcgcccgaga tcggagaggc gcgcgaaacg
    16261 ctggccatca actacaaggg cccggatctc gcggtcgcat tcaatccttc ctttctcctt
    16321 gatgcgttga aagccctgga taacgacgaa gtcttcctgg aactgagcga cgagttgagc
    16381 ccgggggtcc tcaaggtgaa cgggccgttt ctctacgtga tcatgcccat gcgaatgaac
    16441 tgagcgcttg aatccaacaa gccccgaaca cgggttcggt ccgagacgcg cccttcggac
    16501 cgcgagcgct cggctctgat gctatgcgtt ggtcctgccg ccgattcgag tttgtcttcc
    16561 cacgcccagc gctcgtgatg ggcattgtga acgtcactcc ggactcgttc tcggacggag
    16621 ggcggtactt tgatccaggg gctgccattg cgcatgcccg ggaactggtg gctgagggcg
    16681 ccgatttcct ggatgtgggg ggtgaatcga tgcgtccgaa cgcgactccg gtgtccgaag
    16741 cggaggagct gcgtcggatt ctgcctgtga tcgaggagtt ggctcgccaa gtgacggtgc
    16801 cgctgtccgt cgacacgcgc aaaagcggcg tggcgcgggc cgctctggcc gccggggcga
    16861 gcctggtcaa cgacgtcgag gcgaggcgca gcgatgacgc gctctggcgc gtggtggcgg
    16921 atgcccaagc cgggtacgtg tgcatgcaca tgcagggaac accggccacc atgcagcaga
    16981 accccacgta cacggacgtc gtccgtgaag tgggcgagtt cttcacagac cgcttggcgc
    17041 gtttggccag gtgcggcgtc ggacccgacc aggtcgcgtt ggacgttgga atcggattcg
    17101 gcaaggatct ggaccataat ttggatttgc tcgccggcct cgaatcctat aaaggcttcc
    17161 accgaccgtt gttgctcggc gtttcgcgca agtcctttct cggtcgtctg ctgggggtag
    17221 acttggacgc tcgattaccg gcggcgctgg cctgcacggc gtgggcggcg aatgccggcg
    17281 tccaagtgtt tcggacgcac gacgtggcgg cgacgtgcca ggccctgcgt gtgatcgagg
    17341 cgttgcgcgc cagggcggcg acggctgcgt gcccaggaca ttaatggatt ggctgccaaa
    17401 agattggcgg gcgttcgtcg agattctcat tctcgcgatc ggcatctact acgccgtgat
    17461 gctggtgcgc gggacccgcg gcgcgccggt ggtgaccggg ttcctcctgg tgctcatggt
    17521 gctgaccgtg cttgcgcggg ccctcgatct cgaggtcttc gagggtcttc tgggcaactt
    17581 cttcgcgttc ttcgccgtgg gcgtgatcgt catttttcag cctgagctgc gccgcgtgct
    17641 cgccgaactc ggaagcctcc ccttcttcgc cagcacccgc gagcagcggg agaacatcga
    17701 ggtcatcatc aaaacggcgg agcgcctggc ggacgtgcgg atcggcatgc tgctcgccat
    17761 cgaacaatcg atccagctcc aggaagtcgt cgagtcggga atcgtcgtcg atgccgaggc
    17821 cacgcccgag atgctcgaga cgatcttctt ccccaacaac gcgatccacg acggcggagt
    17881 catcctgtgc ggcaaccgca tcatgaacgc cgcgtgcatc ttccccctca cccagcggca
    17941 ggacctcagc aaatcgctcg gcacgcggca ccgggcggcc atcggcctgt cggaggagac
    18001 ggacgccgtc gtcgtggtgg tctccgagga gaccggggcc gtgtcctatg cttacaaggg
    18061 gcagttgatc cgcggagtca cagccgagga gctgcgttct ttcctcacct cagtcctggt
    18121 gcgaatcccc cgctcgcgca ccgtggggga gtggctgcgg accctcgtga cccgcgagcc
    18181 cgccgccggc gtcgccaaac ccgcgcgggc taaaacggac tgatcgtatg cgcctgcccc
    18241 ggattctgac gcacaacttg cggtggaaga tggtcgctct tctttccgcg gcggtggtct
    18301 ggatgacgct caagtcgggg acaccgtcgc gcaatcagcc cagagggtcg cgcgtgctcc
    18361 gccaactgcc gatcacaatc atgaccagca cggaggatcg gcgtgccgtg cgggtggatc
    18421 ccaacactgt ggacgtgcag gtcagcgggg cgcccgaagt cgtggaccgg ctcgaggcgc
    18481 gcgacatccg cgtgtttgtc gatctggccc cgatcgaaga cgaacgtcaa ttccgattga
    18541 gagtcgaggt ccatacgccc tttggcgtca ttctcgaaaa agtcacccct tcggaagtgg
    18601 acggacaaag cctgccgccg aatcctaccc cgcagagtcg acagtgattg cctggctgcg
    18661 ctgcgcagtt ggcggcatga tcacggcatg aagatcaggc tcacccattg agtcgcagac
    18721 tatgtgaact tgccgaaccg ctcggtcaag ggagaatcag tgattggcaa ggcagggatg
    18781 tccgtgcgat gcttcaacac aatgaacatc acaaacatca cacgattggg cttgattgca
    18841 ttcgtcagtc tcctcgcggg ttgcgcaaca tccaacttgc caaagggcgc tagactcgtc
    18901 ggcggtggcc tgagcgtcga atacaccgct cctcgggacg ggacggcgat cctggtcgag
    18961 cgaacctcag gacggatagt ggctaccgag tccctgggag aaggggattc attcgacttt
    19021 aatcccgacg ttgagaatta cgacgaggtt ctattcagga tgtttgggga catccatgcc
    19081 gcgtcggatg ggagtactat ggttgttatc cccacgaacg cgctattcca gctctacttt
    19141 gttcctgaga aaacgaggaa ggaatgatct gccaaccagt cactggagcc gccccgcttc
    19201 tccgctcgaa gtcgggcggc agttcgaacg tgcatcgtgc gctccaccgt ccctgtcgtc
    19261 cctgtcggcg gctgtcgctc agttctgtcg ttcactgtca tccggccatg gtgagtagga
    19321 tctgctggat ccttttccaa atagggatga tggagctgga aagaggtgtg agcgatttga
    19381 gcgcgatttt ggcatttcgc gaagcaggtt ccctgccaaa ctcccgaaat gcatatcgga
    19441 agaaacgttt tcgctcaagt catggcctgg gttcatcccg agcagttccg ccgttgtgtc
    19501 gtccgttacc gcgctgacta caaggttctg acttcccgtc gatccagtcg acaaaccact
    19561 ctcagtccct tggccgtcgt cgtgctggcg atcaaattca cgatcgtctg gctggccttc
    19621 ccccattggc tgcagtttcc cgaccgggtc tgagccggtg taagtc
//
LOCUS       DAOQRO010000011        19428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_1949_length_19428_cov_4.749961, whole genome shotgun sequence.
ACCESSION   DAOQRO010000011 DAOQRO010000000
VERSION     DAOQRO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19428
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_19428_cov_4.749961"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1599)
                     /locus_tag="PKK20_00900"
     CDS             complement(202..1599)
                     /locus_tag="PKK20_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HNU98480.1"
                     /translation="MKTRFLLLPTALILAAAPVSRAAAVVSIGHSPAGSAFVFDSVPI
                     PARNDAASAAEFTLVDGERDSNGGLLAVLHDGRLPVEEDEPAANFFFRAGTDGGRIRI
                     DLKSVIPVRQINTYSWHTGSRAPQVYRLYAADGAASGFVPSPRRGTDPESCGWTHVAA
                     VDTRVRHGAAGGQYGVAVSDPASGKLGDYRYLLLDVVRTEDQDAFGNTFYGEIDVVNA
                     QGPPLEPATGQTGESILIVFGASEGKYRFTIDATAAPDLAEWADAKLRPVVQEWYPKL
                     VAMLPSEGFEPATNITLRFRTDMGGTPASAGGNRVNLNSGWFRRELRREALGSVVHEL
                     VHVVQDYGRARRLNAGATRTPGWLVEGIADYIRWFLYEPETKGAEITERNLDRAAYDA
                     SYRITGNFLNWVTQTHDRDIVPKLNAAAREGRYARALWEDWTGKTLEALGEEWKKGNE
                     ARINAARSSGGTVEK"
     gene            1985..3295
                     /locus_tag="PKK20_00905"
     CDS             1985..3295
                     /locus_tag="PKK20_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNU98481.1"
                     /translation="MTRHAPAIPFILVTLFLDVLGIGLVVPILPRLIEGLSGGNVSSA
                     STTFGLLASLYALMQFIFAPVLGSLSDRYGRRPVILFSLLGSGLDYLVLAVAPTLGWF
                     FVGRIIAGVTGANIAAASAYIADVSPPEKRAQNFGLIGVAFGLGFIVGPALGGLLGQA
                     GLRLPFYAAAALTLLNWCYGLMVLPESLDARHRRAFDWGRANPIGSLAALRRHPVVSG
                     LAGTFFLLHVAHAAVHSTWVLYTAYRYQWTVAQTGVSLAIVGAMAAIVQGGLVRVVVP
                     RLGERRAIVTGLCIGALTMTGYAVATEGWMIYPILVFGSLGGITGPAAQALISKTVAP
                     NEQGAVQGALSSLGSVAGFIGPLSATTLFGYFIGARAPVMLPGAPFYAGALVLLGALA
                     LVSRAFCRSTFHSATPGVGHVVEHGHGHAADQEGSDLPRHERDR"
     gene            complement(3185..6502)
                     /locus_tag="PKK20_00910"
     CDS             complement(3185..6502)
                     /locus_tag="PKK20_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU98482.1"
                     /translation="MNEASHPPSLGLAGRVARAFIDSKLTPLLVVTSILLGLGAIILL
                     PREEEPQIKVPMIDVLVAMPGSNAKEIEERVTRPMEKLLWEIPGVEYLYSTSREGESL
                     AIVRFKVGEEIERSLVKLNQKLQSNFDRIPHGVSTPLIKPRSIDDVPILALTFHSPRQ
                     DHLTLRRLAAQVDDAVKQVPLVAETTLIGGARRQVRVLFDPARLASRNLSLAGVVPML
                     QQANRQSAAGGLTSDNREIAIETGAFLTSAREVGNVVIGVFGGRPVYLRDVADIVDGA
                     EEPSQYVFFGQGQASASLSGPPFAEEPAVTLTVAKRPGANAISVARAVIEKVEALRSR
                     ILPADVAVTITRHYGETAAEKSNELLLHMGIAVVGVAILIWLTLGWRESGIVGVAIPA
                     TLALTLLVFYLYGFTLNRITLFALIFSIGILVDDAIVVVENIVRHFHLPHNKGRSWST
                     IAVEAVNEVGNPTILATFAVIAAVLPMAFVGGLMGPYMRPIPIGSSAAMFWSLAIAFI
                     VTPWASIRILRWGRKYASLTEGAPPAGAAHHSHFEHEEDFFTRLYRRLMGPLIAGSGA
                     RFVFLASITLLLLAAMATVRIGWVKVKMLPFDNKSEFQVILNMPEGSALERTAQAARE
                     IAAAIRTEPEVTDYQVYVGTAAPFNFNGLVRHYFMRRGAHVADLQINLVNKHERKAQS
                     HDIAKRVRPRVAAIAAKHNARVAVAEVPPGPPVLQTLVAEIYGPTAEGRLALAGKVRD
                     IFSQTPGVVDVDWYVEEDQPKARFVIDKEKAALHGISAETISQTLRIAVDGLSVDLVH
                     QPREKEDVNLVLELPRQARTTPEELLALRVRSGDANALPEPGHAGPVPPLVPLRELVR
                     IEQTTADKSIYHKNLMPVTYVIGDVAGEVESPVYAILEMNRALAQIDARQFSGHQARL
                     EILNAAMPFTDHQPAMKWDGEWHITIEVFRDLGLAFAAVCILIYVLMVGWFRSFLTPF
                     IVMVAIPFSLVGILPAHGALNAFFTATSMIGFMAGAGIVVRNSIILVDFIELRLAEGM
                     PLAEAVVDAGAVRFRPMLLTAMAVVVGAAVILFDPIFQGLAISLMAGEIASLLISRMA
                     VPVLYYMAYARGGAVKR"
     gene            complement(6508..7446)
                     /locus_tag="PKK20_00915"
     CDS             complement(6508..7446)
                     /locus_tag="PKK20_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU98483.1"
                     /translation="MNLPSVSVRARPVEAKRWLATEEVVGTIRARVRATLEAKISGRI
                     GQLPAVAGQNVKKGDLLIQLEVSEVQARLDQARAVEQQAAQDRKRFETLLAQKAVTQA
                     EYDAVQARAEVAAASLAEAETMLGYARITAPFDGVISRRLAEVGDLASPGRPLLELED
                     PGSLRLEADVPEALIDRVQLGITLPIRVGARTNALEGVVSELAPMADPNTRTFRVKLD
                     LPPESGLRLGQFGRVAVPVNEITSTRIPSSALVVRGQMEMVFVVVDQHARLRLVKTGK
                     QIGDEIEIVSGLEVGEAVVVEGGVALVDGQPVEVKP"
     gene            complement(7550..8857)
                     /locus_tag="PKK20_00920"
     CDS             complement(7550..8857)
                     /locus_tag="PKK20_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU98484.1"
                     /translation="MLRLIAVVALGASLSAAASEPWTLERALSHTLANSPDARLARQR
                     IVAAKAALDQAKAAFWPQVQVQSSYMGSDNPMLVFGSILNQEAFSPSLDFNRVPDVDN
                     LNVKGLVTAPLYTGGQMASRRKAARANSQATEFEAEAVRNALAFEVARAFHTIHKARA
                     FIEATEAAVRAYESNLAIAQRRFEAGTLLHADVLDVEVRLAQAREDLVRSRNALALSE
                     RALRNLIGLEETEFAVSLDVPSVAPPDAGEVGTRPELAAARSRENAAEASVRAARGGY
                     YPTLSAFGSLDYDRGWRLEGEGESWTAGALLQWNLWDGQLTRAKVAEARASQESANEQ
                     ERKVRLAIDLEIEQARIGLNESTERLQVTEKAVAKAAESVELTRARFEQGLCLASQLL
                     DAETALTGTRVRRAEAEADRRIAIAALRKALGLPQLQTDSASK"
     gene            complement(8845..9054)
                     /locus_tag="PKK20_00925"
     CDS             complement(8845..9054)
                     /locus_tag="PKK20_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNU98485.1"
                     /translation="MTIENAIRVLAGTLVLLSLALAHWVNPWWLLLAAFVGLNLIQSA
                     FTGFCPAETILRKLGVGKEKSGCCG"
     gene            9515..9880
                     /locus_tag="PKK20_00930"
     CDS             9515..9880
                     /locus_tag="PKK20_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98486.1"
                     /translation="MRLELALCGVLIAGLPMLSRHFGLDVRLTTVVLGLAAGGLCLLW
                     SVLSRRRRCVLWGAIPTLAAATLVFVGQAFESRHAAPALEAKGRMTVAVMAVLSVFCA
                     GTVVNLTRRASDQVRSSFP"
     gene            10037..10729
                     /locus_tag="PKK20_00935"
     CDS             10037..10729
                     /locus_tag="PKK20_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNU98487.1"
                     /translation="MKTIGMIGGMSWQSTIEYYRLVNESIQKKLGGLHSAKCVLYSVD
                     FAEIEEWQRHGEWNKAAEALVGAAQGLEEAGADFVLICTNTMHKLADAVQSRIGIPLL
                     HIADATAEAVRRAGLRRVGLLGTRFTMEEEFYRSRLANQFGLEVVVPDAEDRQTVHRI
                     IYEELCAGAVRPESKALVGGIMNRLEAVGAKGVILGCTELGLLVDAGDSPLPLFDTTR
                     LHAIAAVDWALS"
     gene            10733..12079
                     /locus_tag="PKK20_00940"
     CDS             10733..12079
                     /locus_tag="PKK20_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNU98488.1"
                     /translation="MPNSVQSQLLDSVPDGVFTVDAEWRITTFNRSAERITGIHREEA
                     LGRRCCDVFRASICESACAVKQTLATGRPVVNKVVYIVDASGQRLPIGVSAAVLKDSR
                     GRVIGGVESFRDLRVVEELQRQARAQDSCSGLIGRSARMRELFELLPVVAASDSTVLI
                     EGASGTGKELVARAIHAHSPRRTHPFLAVNCAALPDTLLESELFGYKAGAFTDAKKDK
                     PGRFALAQGGTILLDEIGDISAALQTRLLRVLQERVYEPLGSVVAVKADVRVIAATNK
                     DLRQLAQEGRFREDLFYRIHVVRLELPRLRDRREDIPLLTEQFIARFNRLQGRDIAGV
                     SEGVIARLMEHDFPGNVRELENAIEHAFVLCRGALIEECHLPPHLRAAESEQGTRLPR
                     GLTLAAMEELLLRDALRRHGGNRAAAARELGINTSTLFRKIKALKLDRIGKGDRNP"
     gene            12409..12831
                     /locus_tag="PKK20_00945"
     CDS             12409..12831
                     /locus_tag="PKK20_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNU98489.1"
                     /translation="MAIPIWQERVSPVFDAASRLLVVRRHRGRTVERKEFVLTATSVE
                     ALARSVAELGIDVLLCAAVSETLRTALERAGVAVEAHLCGQVEDLLEAFHAGRWRRSE
                     FRMPGCWDPHGPLRLRRGGCRSRRRMQPAERAKSDQKA"
     gene            12828..13670
                     /locus_tag="PKK20_00950"
     CDS             12828..13670
                     /locus_tag="PKK20_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU98490.1"
                     /translation="MNNTIAVTVLVENTARDRGLMGEHGLAYYVQAGGRTLLFDTGQT
                     SLIIQNARRLGIELSGLDAVALSHGHYDHTGGLHAVLGIARDAELYTHPAACARRFAR
                     NPDGTTRDVGIGRESLDSIQARPERIHETTTATEVAQGVFLTGEIPRATDFEDVGGPF
                     VLDRRGLEADPIVDDQALFFDTADGVAVLLGCAHAGIVNTLRHIRQLTGGRPIHSVLG
                     GMHLLAAGPERMNRTVEALRGEKVRRLGPAHCTGMAATSRLWHEFPQACTVCAVGSRF
                     TFER"
     gene            13704..15155
                     /locus_tag="PKK20_00955"
     CDS             13704..15155
                     /locus_tag="PKK20_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU98491.1"
                     /translation="MKLPHALGLGLALGLSGLPVWAQDSAQNSTVEQLQQQLLQLRRQ
                     FEETQRQQSAQIEALQRQIEALQVKPPVEATAPAPAAAAPPAHPAPVADAAPAQAAIQ
                     TWTPAQPIPVMRSGQDYLNLSFKMLATAGLSTSTEAGEEIELGHHDPAGRGFTLQAAE
                     IALDGAVDPYFRGFGNICMSVDPDGETHVDLGEVYLLTTALPANLQVKAGLFLAEFGR
                     QNQQHAYQWDFVDQPIILNRLLGPHSLRDPGARLSWLIPTPFYTELMLGVFHGQGHSA
                     FSFRSHDYDGTHGRTSLERELHGPGDLLYVPRITSSFDLSDTQTLVAGASGGFGPNNS
                     GADADSQIYGLDLYWKWKPVHAEHGFPFLAWQSEGLYRRYEAGADSERDMPSEDLDDW
                     GFYSQIMWGFKRRWVAGLRGEWADGNTGAWDASDRFRGERIRFSPNLTCYPTAFSKIR
                     LQYNLDDGDRFGTEHSVWLQGEFLLGSHGAHKF"
     gene            15169..16131
                     /locus_tag="PKK20_00960"
     CDS             15169..16131
                     /locus_tag="PKK20_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNU98492.1"
                     /translation="MNRLDQPLANSRARWPSLLLAACVLSSPTAWAKLRVVTTTPDFA
                     AIAAEIGGDRVEVTSLGKPTEDPHFIDARPSLILKLNRADVLVEGGAELEIGWLPSLM
                     DSARNPKIQRGAPGNVYASEGIEMLEVPSVLDRSMGGVHPVGNPAFMTDPVNARIVAE
                     RLARVFGQLDSKSSALFNANLKSFSDKLAGKLIEWRNRLAPFKGSRIVAYHNSWPYFA
                     RRFGLQVDLFLEPKPGLPPTPTHLAQVMAKMKESGARVILVQPYLNRHTAETVASHTG
                     AVVVDAAGFPGGVKGTEGDYCKFMDHLVNSLADALGGKTPSTQQ"
     gene            16137..17030
                     /locus_tag="PKK20_00965"
     CDS             16137..17030
                     /locus_tag="PKK20_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNU98493.1"
                     /translation="MEPINILEMLVILKWPLIASLTLPWLLVYLGLHVVQREIIFVDL
                     ALAQVAALGTCVSLLMDCHLHSWQSYAWSAAFTVVGAVVFTLTRSRHPRVPQEALIGI
                     VYVVAAAAGILILMRSTGGKEELQRSLVGELLVVPPREVIQTIGLYLGIGAVHYVFRR
                     KFLAISKDPDAAVRSGLSVRGWDFLFYVTFGFVVTSFVHIGGVLLTFSYLIVPAVCGN
                     YLAHTLRRRLAIGWTVATLASLASLCLTPALDLPVGATTVCMLGLLLAITMALARRVP
                     APQTRPAAADTLTVARSQSDA"
     gene            17040..17261
                     /locus_tag="PKK20_00970"
     CDS             17040..17261
                     /locus_tag="PKK20_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98494.1"
                     /translation="MNQKQYSKLDVTLASVCRSCPVCRTARRRRTGLAYWLVNRVESS
                     LCPFCRAYERVYGRKSHETGAAHQPQSDT"
     gene            17258..18505
                     /locus_tag="PKK20_00975"
     CDS             17258..18505
                     /locus_tag="PKK20_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU98495.1"
                     /translation="MIDRCTLWLATALTLVCQAGSLAAGGSRAQALKALCGRLEIGPG
                     AVIADVGCGEGLDAAEFARVVGESGTVLAQEIEPAKLATVVRVAAKHGLHQIVPVLGQ
                     SDDPRLPDGFVDLIYLNRVFHHLAQPQAMLRRLHQDLKPGGLLVVVDQHRGPLTDWAP
                     TERREEEHHWTGETTVVRLAREAGFLFHDVLEDVWHEPQPFVLAFRKPAAPPPDAGDP
                     DLPPPLDAKALVRALPIPQQDGWAAIFVGLDRGRAVLPALKEGLPPSTRLADVVLDEW
                     ALTLEELPPGAPHPGTEVLRKNNNSLALPPDMRLGLVLFVDAYQWLWEPVPLLRQLRE
                     RMPDSGLLAIVERQGPDDEPRRLAGHHRRISSRRVAEEMREAGFQLRQTLAPPTEDRC
                     FLLFEPRTSHERLQPEAPKELRP"
     gene            18765..19169
                     /locus_tag="PKK20_00980"
     CDS             18765..19169
                     /locus_tag="PKK20_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNU98496.1"
                     /translation="MKIAIPLVAGRLSEHFGHCEQFALIETDLESRQMVSQTRMVPPP
                     HAPGVLPRWLHEQGAKVIIAGGMGRRALDLFIQNGIAVHTGIAGASPEELAHAFLTGA
                     LGDTTPTCGHRRGDERHRHHGCHHDAGSSHEP"
     gene            19166..>19428
                     /locus_tag="PKK20_00985"
     CDS             19166..>19428
                     /locus_tag="PKK20_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNU98497.1"
                     /translation="MKIAISTQGPQPNSALEPRFGRARYFRIVDTETGQQTVVDNENA
                     AGAVQGAGPRAVQMLARLGVQAVLTGHVGPKAWTALESSKIQA"
BASE COUNT         3396 a         6299 c         6269 g         3464 t
ORIGIN      
        1 ccgaagacca gcggcgcaaa ggcccggacg agactcggga actcaggtcc gccgggcgcc
       61 atggaccggg cacggcgcag gtcgcgaatg gtcattgcga cgtgaatgcg gcaggtcccg
      121 ccgtgcgcgg gcgcgcggcc cgaagattcc cgtggaccta atgtctcaga cgcgtcgcgg
      181 acggcggcta ttgtatcggt ctcatttctc aacggttccc ccgctggatc gcgcggcgtt
      241 tattcgggct tcgttgccct tcttccattc ctcgcccagc gcttcgagcg tcttgccagt
      301 ccaatcctcc cagagcgccc gggcatacct tccctcgcgc gcggcggcgt tgagcttggg
      361 cacgatatcc cgatcgtgcg tctgggtcac ccaattgagg aagttgcccg tgatgcggta
      421 gctggcatcg taggcggcgc gatctagatt gcgctcggtg atctcggcgc ccttcgtctc
      481 cggctcgtaa aggaaccagc ggatgtagtc ggcgatgcct tcgaccagcc agccgggggt
      541 gcgtgtggcg cccgcgttga ggcgtcgtgc gcgaccgtag tcctggacga catggaccag
      601 ttcatgcacc acagagccga gcgcctcccg gcgcagctcg cgccggaacc atccgctgtt
      661 caggttgacg cggtttccgc ccgccgacgc cggtgtgccg cccatatccg ttcgaaaccg
      721 cagggtgatg ttcgttgcag gctcgaagcc ttcgctgggc agcatcgcga ccagcttcgg
      781 ataccattcc tggacgaccg gccgcagttt ggcatccgcc cactccgcca ggtccggcgc
      841 ggccgtcgca tcgatggtga acctgtattt gccctcggac gccccgaaca caatgagaat
      901 ggactcgccc gtttgacccg ttgcgggctc gagaggcggc ccctgggcgt tgacgacgtc
      961 gatctcgcca tagaaggtgt tgccgaacgc gtcctggtcc tcggtgcgga cgacgtccag
     1021 caacagatac cggtagtcgc ccagcttgcc cgaggcgggg tccgacaccg ccaccccata
     1081 ctggccgcct gcggccccat gcctgacgcg ggtgtccacc gccgcaacat gtgtccaccc
     1141 gcacgattcg ggatcggttc cccgacgcgg gcttgggacg aatccggagg ccgcgccgtc
     1201 cgccgcgtag agccggtaga cctgcggagc gcgcgatccc gtgtgccacg agtacgtgtt
     1261 gatctgcctc accggaatga cgctcttgag atcaatccgg atgcggccgc cgtccgtccc
     1321 ggcgcggaag aagaagttcg ccgcaggttc gtcttcctcg acgggaagcc gtccgtcgtg
     1381 caggacggcg agcaggccgc cgttcgagtc gcgttcgcca tccaccaggg tgaactccgc
     1441 cgcggacgcc gcatcgttcc tggcggggat cggcacagag tcgaaaacga aggcggaccc
     1501 ggcggggctg tgcccgatcg agacgacggc cgcggctcgg gagaccggtg cggcggccag
     1561 aatgagagcg gtaggaagga gcaggaatcg ggtcttcatg tcgaatgcgt gatattggac
     1621 caagccaacg cgcgccgtcc agtgaatgcg gttggacggg ccggaccgcg gtgcatctgg
     1681 ttgttgtcgg tgttctgtcg cccggtgagg gcgtcgggcc tacaacggat ggagattgcc
     1741 ggagaggcga cgcctgcctg tcgcctgcct gccgactgcc tgccgccagg cagggctggc
     1801 agggctggca gggctggcaa gtcctcgtcg cccgccaaac aggatctgca actttcatcg
     1861 ccggggacga gacgttcccc cgccgccctt ttcgtcatca tgaattgttc cggcgctgag
     1921 cgcactccgc gcactctacg ctcgccaaga gaatgatctg ggccaagagt ggcttcgagt
     1981 ctttgtgacg cgccacgcgc cagctattcc gttcatcctg gtgaccctgt tcctggatgt
     2041 gctgggcatt gggctcgtcg tcccaatcct gccgcgcttg atcgagggtc tgtcgggagg
     2101 gaacgtgtcg tccgcctcga ccacattcgg cctgttggcg tcgctctatg cgctgatgca
     2161 gttcatcttc gcaccggtgc tgggaagcct ctccgaccgc tacgggcggc gtcctgtcat
     2221 tctgttttcg ctgttggggt cgggactgga ctatctcgtg ttggcggtgg cgccgacgct
     2281 gggatggttt tttgtggggc gcatcattgc gggcgtgaca ggggcgaaca ttgcagcggc
     2341 cagcgcgtat attgcagatg tgagcccgcc cgagaagcgg gcgcagaatt tcggtctgat
     2401 cggtgtggcg ttcggactcg gattcattgt ggggccggcg cttggcggcc tgctgggtca
     2461 ggccgggctg cggctgccgt tttatgcggc ggcggcgctg acgctgttga actggtgtta
     2521 cgggctgatg gtgttgcccg agtcgctcga cgcgcggcat cggcgggcat tcgactgggg
     2581 gcgggccaat ccgatcgggt cgctagcggc cttgcggcgg catccggtgg tatccgggtt
     2641 ggcggggacc ttctttttgc tgcacgtggc gcatgcggcc gtgcacagca cctgggtgct
     2701 gtacaccgcc tatcggtatc agtggacggt ggcgcaaaca ggggtgtcgc tggccatcgt
     2761 gggagcgatg gccgcgattg tccagggcgg tctggtgcgc gtggtggttc cgaggctcgg
     2821 tgagcgccgt gccattgtga cgggtttgtg catcggggcg ctcacgatga ccggttacgc
     2881 ggtggcaacc gagggttgga tgatctatcc catcctggtt ttcggatcgt tgggcggcat
     2941 caccggcccg gcggcccaag ccctcatctc gaaaaccgtc gccccgaacg aacaaggggc
     3001 tgttcagggg gcattgtcga gtctgggaag cgtggccggg ttcatcggac cgctgtcggc
     3061 cacaacgctt ttcggctact tcatcggcgc gcgcgcgcct gtgatgctac ccggggcccc
     3121 gttttatgcg ggcgcactgg ttctgcttgg agcgctggcc ctggtgtcgc gcgcgttctg
     3181 caggtcaacg tttcacagcg ccaccccggg cgtaggccat gtagtagagc acgggcacgg
     3241 ccatgcggct gatcaggagg gaagcgatct cccccgccat gagcgagatc gctaaaccct
     3301 ggaagatcgg atcgaacaga atcaccgccg cgcccaccac cacagccatg gccgtcagca
     3361 acatcggacg gaagcggacg gcccccgcat cgaccacggc ttcggcgaga ggcatgccct
     3421 cggcaagacg cagctcgatg aaatcgacga gaatgatcga gttgcggacg acgatgccgg
     3481 cgcccgccat gaaaccgatc atcgaggtgg cggtgaagaa ggcgttgaga gcgccgtgcg
     3541 ccgggagaat ccccaccagc gagaacggga tggccaccat cacgatgaac ggcgtcagaa
     3601 acgatcggaa ccacccgacc ataagcacgt agatgaggat gcacacggcg gcaaaggcaa
     3661 ggcccaggtc gcggaacacc tcgattgtga tgtgccattc cccgtcccat ttcatggccg
     3721 gttggtgatc ggtgaagggc attgccgcat tgagtatctc gagccgggcc tggtgcccgc
     3781 tgaattgccg ggcatcgatc tgcgcgaggg cacggttcat ctcgagaatc gcatagacgg
     3841 ggctttcaac ctctcccgcc acatccccga tcacgtaggt caccggcatc aggttcttat
     3901 gataaatgct cttgtcggca gtggtctgct cgatccgcac cagctcgcgc aatggcacca
     3961 gcggcggcac gggaccggcg tggcccggct cgggcagggc gttcgcatcc ccgctgcgca
     4021 cccgcaacgc gagcaactcc tccggcgtgg tgcgggcctg acgaggcagc tcgaggacca
     4081 gattgacatc ctccttctcg cgcggttgat ggacgaggtc gacggagagt ccatcgacgg
     4141 cgatgcgcag ggtttgcgag atggtctcgg cgctgatgcc gtgcagggcg gccttctcct
     4201 tgtcgatgac gaatcgcgcc ttgggctggt cctcctcgac ataccagtcg acatcgacga
     4261 cgcccggcgt ctggctgaag atgtcgcgca ccttgcccgc cagagccaga cgtccctccg
     4321 ccgttggccc gtagatctcc gcgacgagcg tctgcaatac gggcggcccc ggggggacct
     4381 cggccaccgc tacccgggcg ttgtgcttgg cggcgatcgc agccacgcgg ggccggacgc
     4441 gcttggcgat gtcgtggctc tgggccttgc gctcgtgctt gttgaccagg ttgatctgga
     4501 gatcggcgac atgggcgccg cggcgcatga agtagtgccg gacgaggccg ttgaagttga
     4561 acggggcggc ggtgccgacg tagacttggt aatcggtcac ctcgggttcg gtgcgaatgg
     4621 cggcggcgat ctcgcgggcg gcctgggccg tccgttcgag cgcgctgcct tcaggcatgt
     4681 tcagaatcac ctggaattcg ctcttgttgt cgaacgggag catcttcacc ttgacccagc
     4741 caatgcgcac ggtggccatc gcggccagca gcaaaagggt aatggatgcc agaaacacga
     4801 accgcgctcc ggagcctgcg atcaacgggc ccatcagtcg ccgatagagc cgcgtgaaga
     4861 aatcctcctc gtgctcgaaa tgcgagtggt gcgcggcccc ggcgggcggg gcgccctccg
     4921 tgagcgaggc atacttccgg ccccatcgca agatgcggat cgacgcccac ggcgtgacga
     4981 tgaacgcaat cgccagcgac cagaacatcg cggcgctcga gccgatgggg atgggccgca
     5041 tgtagggccc catgagcccg ccgacaaagg ccatgggcaa tacggcggcg atcaccgcaa
     5101 acgtcgccag aatcgtgggg ttgccaacct cgttcaccgc ctcgacggcg atggttgacc
     5161 aggagcgccc cttgttgtgg ggcaagtgaa agtgccggac gatattctcg accaccacga
     5221 ttgcatcatc gacaaggatg ccgatgctga agatgagcgc gaagagcgtg atccgattca
     5281 gagtgaaccc gtaaaggtaa aagaccaaga gcgtcagggc gagcgtcgcc gggatggcca
     5341 cccccacgat gcccgactcg cgccagccca gcgtcagcca gatcaggatg gccacgccca
     5401 caaccgcaat gcccatgtgc aagagcagtt cgttcgattt ctcggcggcc gtctcgccat
     5461 aatgccgcgt gatggtgacg gcgacgtcgg cgggcagaat gcgggaccgg agcgcctcga
     5521 ccttctcgat caccgcgcgc gcaaccgaga tggcgttcgc cccgggccgt ttggccaccg
     5581 tgagtgtcac ggccggctcc tccgcgaaag gcggacccga caacgacgcg cttgcctgtc
     5641 cctggccgaa gaagacgtac tgacttggct cctccgcgcc atccacaatg tccgccacgt
     5701 cgcgcaggta aaccggccgg cctccgaaaa ccccgatcac gacattgcca acctcgcgcg
     5761 cgctggtgag aaacgcgccg gtctcgatgg cgatttcccg gttgtcgctt gtgaggccgc
     5821 cggcggcgga ctggcggttg gcctgctgga gcatcgggac cactccagcc aggctcagat
     5881 tgcgcgacgc caggcgggcg ggatcgaaca ggacccggac ctggcggcgc gccccgccga
     5941 tgagcgtggt ttcggcaacg agcgggactt gcttcaccgc gtcgtccact tgcgccgcca
     6001 accgccgcag agtcaggtga tcctgccgcg ggctgtgaaa ggtcagggcg agaatgggga
     6061 cgtcgtcgat cgacctcggt ttgatcaagg gcgtgctcac gccgtgcggg attcgatcga
     6121 aattcgactg cagcttctgg ttcaacttga ccaggctgcg ctcgatttcc tcgcccacct
     6181 tgaaccggac aatcgccagg ctctcgcctt cgcgggacgt cgaatagagg tactcgacgc
     6241 cgggaatctc ccaaaggagc ttctccatgg gccgagtgac gcgctcctcg atctccttgg
     6301 cattcgagcc gggcatcgcg accagcacgt cgatcatcgg caccttgatc tgcggttcct
     6361 cctcccgcgg gagaaggatg atggcgccca ggcccagcag aatggacgtg acgaccagga
     6421 gcggggtcag tttcgaatcg atgaacgccc gggccacccg accggccaga cccagcgagg
     6481 gcggatgcga tgcttcgttc aaagcgctca tggcttcacc tcgacgggtt ggccgtccac
     6541 taaggcgacg ccaccctcga cgacgactgc ctcgcccacc tccaatccgg acacgatctc
     6601 gatctcgtcg ccaatctgct tgcccgtctt gacgagccgc aaccgggcat gctggtcaac
     6661 gaccacgaac accatctcca tttggccccg caccacgagg gcggaggacg gaatccgagt
     6721 cgacgtgatc tcgttcaccg gcacggccac ccgaccgaac tggcccagac gaagcccgct
     6781 ttcaggcggc aaatcgagtt tcacgcggaa tgtgcgtgtg ttcgggtccg ccatgggagc
     6841 cagctcgctg acaacgcctt cgagggcatt ggtccgcgcc ccgacacgaa taggaagtgt
     6901 gatccccaac tgcacccggt cgatcagggc ctccggaaca tccgcttcga gccgcagcga
     6961 ccccgggtcc tccaactcga gcaacggacg cccgggggac gccaggtcgc ccacctcggc
     7021 caaccggcgg ctgatgacgc catcgaacgg agcggtgatc cgggcatagc caagcatcgt
     7081 ttcggcctcg gctagggaag cggcggcgac ctcggcgcgc gcctggacgg cgtcgtactc
     7141 ggcctgagtc acggcctttt gagcaagaag agtctcgaat cgcttgcgat cctgggcagc
     7201 ctgctgctcg accgccctgg cctgatccaa ccgagcctgg acttcactga cttcgagttg
     7261 aatgagcaga tcgcctttct tgacgttctg gcccgcgacc gcaggcaatt gaccgatccg
     7321 cccgctgatc ttggcttcga gcgtggcgcg gacccgggcg cggatggtgc cgacgacctc
     7381 ctcggtcgcc agccaccgct tggcttccac cggtcgggcc cgcaccgaga cggaaggcaa
     7441 attcacctcg gaagatgcgt cctgcttccg gccgcagccg ggagtcaggg ataggatcag
     7501 aatggctagg gcgcaggtgg ttgttagcgg aaacgcgagt ttggtcatat catttagatg
     7561 cggagtcagt ctgaagctgc gggaggccca gcgccttccg cagagcggcg atggcaatcc
     7621 gacgatcggc ctcggcttcg gcccgccgca cgcgcgtgcc tgtcagggcg gtctcggcgt
     7681 ccaaaagctg actggccagg cacagccctt gctcgaagcg agcgcgtgtg agttcgacgc
     7741 tttctgccgc cttggcgacg gctttctcgg tgacttgcag acgttcggtg gactcgttca
     7801 aaccgatgcg cgcctgctcg atttccaggt cgatcgcgag cctcactttg cgctcctgct
     7861 cgttggcgga ttcctgggaa gcacgcgcct cagccacttt tgcacgcgtc aattgaccat
     7921 cccagaggtt ccattgcagc agagcgcccg cagtccagct ctccccctcg ccttcgagcc
     7981 gccagccccg gtcgtaatcg agactgccaa aggcgctgag cgtcgggtaa tacccccctc
     8041 gagccgctcg gacgctcgct tcggcggcgt tctcacggga ccgggcggcc gccagttcgg
     8101 gacgcgtgcc cacttcgccc gcatccggag gcgcgacaga cggcacatcg agcgacacgg
     8161 cgaattccgt ttcttcgagt ccgatgaggt tccgcaaggc gcgctcggag agggccaacg
     8221 cgttgcggga ccgcaccagg tcttcgcgcg cctgggccaa acgcacctcg acatcgagca
     8281 cgtcggcgtg cagcagtgtg cccgcttcga atcggcgctg ggcgattgcc agattgctct
     8341 cgtaggcccg gacggcggcc tccgtggcct cgatgaaggc gcgggccttg tgaatggtgt
     8401 gaaaagcccg cgccacttca aacgccagcg cgttgcgaac cgcctccgcc tcgaattcgg
     8461 tcgcctgcga gtttgccctg gctgccttgc gcctggatgc catttgcccc ccggtgtaga
     8521 gtggcgccgt caccagtccc ttcacattca gattgtcgac gtccggtact cggttgaaat
     8581 cgagcgacgg gctgaatgcc tcctggttga gaatcgaccc gaacacaagc atcggattgt
     8641 ccgagcccat gtaactcgac tggacttgca cctgcggcca gaaagcggcc ttggcctgat
     8701 ccagcgcggc cttggcggcg acaatgcgct gccgggccag tcgcgcatcg ggactgttgg
     8761 caagcgtatg gctcaacgcg cgctcgaggg tccacggctc cgatgcggcc gcgctcaagg
     8821 acgcgcccaa agccacaacg gcaatcaacc gcaacatccc gacttctcct ttcccacccc
     8881 gagcttccga agaatcgtct ccgccggaca gaaccctgtg aaagccgact ggatgaggtt
     8941 gaggccgacg aaagcggcca ggagcaacca ccacgggttc acccaatggg ccaaagcgag
     9001 actcagaagt accagagtgc ccgccagaac gcgaatggca ttttcaattg tcataggtcg
     9061 ctgaattgag gttcacgatt cagtttgccc gtatcgagcc gcgagccgca aagaagttac
     9121 gcaccgcatg gcccacaacg cccgccttcc tgcgcgccga gccccaattc tcacaagccg
     9181 tctccctcgc aaggccaaac ctgccttagg gtaattgatt tttgcacaga ccctaaccgt
     9241 ctcgcgcagc aaagacgaga atcagcgccg actcatcccg cgatgtggaa cgaggacacg
     9301 gatgtccggg ctgcagccgt ccaacggttt gtttacgccc agccttgtcg cccgtcaaca
     9361 accgccgacg ccgtcggcgg cgaagatgcg gcttggccgc cgacgcgtgg cgatggtaga
     9421 aaagggtcgt tgagacatga ccgaaaaccc gccagtgaca agtggcgctg cgccgttcca
     9481 ggcagcgcgg aggcgatctc tctcacccgt cgaggtgagg cttgaactgg cgctctgcgg
     9541 agtgctgatc gccggcttgc cgatgctctc ccggcacttc ggactcgatg tccggctcac
     9601 gaccgtggtt ctgggcctgg ccgccggagg gctgtgcctc ctctggagcg tcctgagccg
     9661 acgcagaagg tgtgtgcttt ggggagcgat tccaacgctc gcggccgcca ccctggtctt
     9721 cgtcggccag gcatttgaat cgcggcacgc cgcgcccgcg ctcgaggcca aggggcggat
     9781 gaccgtggcg gtgatggccg tgttgtcagt gttttgcgcg ggcacagtgg tcaacctgac
     9841 gaggcgcgcc tcagatcaag tccggtcctc atttccatga gaaatccccg tgtttcccgg
     9901 cccctctctc ccgcgcctcg ctcgttagaa tccttgaacc ctccaacgca gcacagaaac
     9961 tcgatctggc tacgacatgt atcaccggtc taggctacca gtccgcgtgg ccagagatcg
    10021 tcgcgagtga acaacgatga aaaccatagg aatgatcggc ggaatgagtt ggcaatcgac
    10081 catcgagtac tatcgactgg tcaacgaatc gattcagaag aagctgggag gcctgcactc
    10141 cgccaagtgc gttctctatt ccgtcgattt tgcggagatc gaggaatggc aacggcacgg
    10201 cgaatggaac aaggcggctg aggcgctggt cggagcggcg cagggcctcg aggaggcggg
    10261 ggctgatttc gtgctgatct gcaccaacac gatgcacaag ctcgccgatg ccgtgcaatc
    10321 gcgcatcggc atcccgctgc tgcacatcgc ggatgcgacg gcggaggcag tccgacgggc
    10381 gggactgcgc cgggtgggtt tgctggggac gcgttttacc atggaagagg agttctaccg
    10441 gagccgattg gcaaatcagt ttgggctcga agtcgtggtg ccggacgcgg aggaccggca
    10501 gacggtgcac cgcatcattt acgaggaact gtgcgccggc gcagtgcggc ccgaatcgaa
    10561 ggcgctggtc ggtggaatca tgaatcgtct cgaagccgtg ggcgcgaagg gcgtgatcct
    10621 gggttgtact gagttggggt tgctcgtgga tgcaggggac agcccgctgc cgctcttcga
    10681 caccacgcgc ctgcatgcca ttgcggcggt ggattgggca ttgagctgac gcatgccgaa
    10741 ctcagtccaa agccaactgc tcgactccgt gcccgacggc gtgttcaccg tggacgccga
    10801 gtggcggatc acgaccttca accggtccgc cgagcggatc accggcatcc atcgtgagga
    10861 ggcgctgggc cgccgttgtt gcgatgtgtt ccgcgcctcg atttgcgaga gcgcctgcgc
    10921 cgtcaaacag acactggcca caggccgccc ggtggtcaac aaggtggtct acatcgtcga
    10981 tgcctcaggc cagcggctgc ccatcggcgt ttcggccgcc gtcctgaaag acagccgggg
    11041 ccgagtcatc gggggcgtcg agagctttcg cgacctgcgg gtggtcgagg agttgcagcg
    11101 ccaggcgcgc gcccaggatt cctgctcggg tctgatcgga cgcagcgcac ggatgcgcga
    11161 gctgttcgag ttgctgccgg tggtggcggc cagcgacagc accgtgctca tcgagggcgc
    11221 cagcggcacc ggcaaagaat tggtcgcccg ggcgattcac gcccactcgc cccgaagaac
    11281 gcatccgttc ctcgccgtga actgcgcggc tttgcccgac acactgctcg aatcggaact
    11341 gttcggctac aaggccgggg cgttcaccga cgccaaaaag gacaagcccg gacgctttgc
    11401 gcttgcgcag gggggcacga ttctgctcga cgagataggc gacatctcgg ctgccctcca
    11461 aacacgcctg ctccgcgtgc tgcaggaacg cgtctacgaa cccctgggca gcgtcgtcgc
    11521 agtcaaggcc gacgtccggg tgatcgccgc cactaacaag gacctgcgtc aactggcgca
    11581 ggaaggtcgg tttcgcgagg acctcttcta tcgcatccat gtcgtgcgtc tcgaactccc
    11641 acgcctgcgg gatcggcgcg aggacatccc cctcctgacc gagcagttca tcgcgcgatt
    11701 caaccggctc cagggacgcg atattgcggg agtctccgag ggcgtcatcg cgcggctcat
    11761 ggaacatgat tttccgggca acgtccggga actggagaac gccatcgagc atgcgttcgt
    11821 gctctgccgg ggcgccttga tcgaggagtg ccatcttccg ccccacctgc gagcggcgga
    11881 gtcggagcag ggaacgcggc tcccgcgcgg tctgaccctc gcggcgatgg aggaactgct
    11941 cctgcgcgat gccctgcggc gccatggcgg caaccgcgcc gcagcggccc gggaactggg
    12001 catcaatacc agcacactct tccgcaagat caaggccttg aagctcgacc ggatcggcaa
    12061 aggcgatcgg aatccttgac cggtgatgcg gaatgccagt gtgtccaatg cgcaccgaag
    12121 caaaaggcaa tgcctcgatc cggccaggca catctcatgc tccggggcga atccttccgc
    12181 gaagaccgag ctttttccgc gggccgcacc ccgcaaagcc attgggttga cgcgcatgcg
    12241 ccgatgcggg agttcgcccc catcgcaggc gggcggcgag ctcgtgagga gctccggagc
    12301 gcggacttac tgccggcttg acgcgcgcga cgtgcccgcc ctcactcgac tccgatcgac
    12361 agccgattgg gcattggcat gacagttgca tgtggtgctg ccatgaccgt ggccatccct
    12421 atctggcaag agcgcgtttc acctgtgttc gacgccgcct cccggctgtt ggtcgtgcgg
    12481 cgccatcgcg gccggaccgt cgagcgcaag gagttcgtct tgaccgcgac gtcggtcgag
    12541 gccctggccc ggagcgtggc ggaactgggc attgacgtgt tgctttgcgc cgcggtttcc
    12601 gagactctgc gcaccgcgct cgaacgcgcg ggggtggccg tcgaggccca tctctgcggc
    12661 caagtcgagg acttgctcga ggcttttcat gccgggcgtt ggcgccgatc cgagttccgc
    12721 atgccggggt gctgggatcc gcatggcccg ctccggctgc ggcgcggcgg atgccgatct
    12781 cggagacgga tgcagcctgc tgaacgcgcc aagtccgacc aaaaagcatg aataacacga
    12841 tcgctgtcac tgtcctcgtt gaaaacaccg cgcgggaccg cgggctgatg ggagagcacg
    12901 gtctggccta ttacgtccaa gcaggagggc gcaccctgct tttcgacacc ggccaaacct
    12961 cgctgatcat ccagaatgcc cggcgactgg gcattgagct atcgggcctc gacgcagtgg
    13021 ccctcagcca cgggcattac gaccacacag gcggcctgca cgccgtgctc ggaatcgcgc
    13081 gcgacgccga gctttacacc catccggcgg cctgcgcccg acggttcgct cgaaatcccg
    13141 acggcaccac gcgggatgtc gggattggcc gggagagcct cgattccatt caggcgcgtc
    13201 cggagcgcat tcatgagact acgactgcga cggaggtagc acagggcgtg ttcttgaccg
    13261 gcgagatccc gcgcgcgacc gacttcgagg atgtgggcgg cccgttcgtt ctcgaccgaa
    13321 gggggctcga ggccgatccg atcgtcgacg accaggcttt gttcttcgac acggcggatg
    13381 gggtggccgt gctgctcggc tgcgcgcacg cggggattgt aaacaccctg cggcacatcc
    13441 ggcagctcac aggcggacgc cccatccaca gtgtgctggg cggcatgcat ctgctggcgg
    13501 ccgggccgga gcgcatgaac cgcacggtcg aggcgttgcg tggggagaag gtccggcgac
    13561 tcggaccggc ccattgcacg ggaatggccg cgacgtctcg gttgtggcac gagtttccac
    13621 aagcctgcac ggtgtgcgct gtgggcagcc gtttcacgtt tgaacgctga acggatcggt
    13681 gcagagtgtc actagacatg atcatgaaac tgccgcacgc tcttggattg ggtctggcac
    13741 tggggctgag tgggctgcct gtctgggctc aggattcagc ccaaaacagc acggtcgaac
    13801 aactgcagca gcaattgctg cagttacgcc ggcaattcga ggaaacgcag cgccagcaaa
    13861 gcgcgcagat cgaggcgctg cagcgccaaa tcgaagcttt gcaggtcaaa ccgcccgtcg
    13921 aggcgaccgc cccggcgccc gcggctgctg caccgcccgc acaccctgcg cctgtggctg
    13981 acgcggcgcc cgcgcaggcg gcgattcaga cctggactcc cgcgcaaccc atcccagtca
    14041 tgcgctccgg tcaagactac ctgaacctga gcttcaaaat gttggcgacg gccggcctct
    14101 ccacgtcgac ggaggcggga gaggagatcg agttgggaca ccacgatccc gcgggacgcg
    14161 gcttcacgct tcaagccgcg gagattgccc tcgacggcgc cgtcgaccca tacttccgcg
    14221 gtttcggcaa catctgcatg agcgtggatc cggacggtga aacgcacgtc gacctgggcg
    14281 aggtctactt gctgacgacg gcgctgccgg cgaatctgca ggtgaaggcg ggcctttttc
    14341 tggccgagtt cggacggcaa aaccagcagc acgcatatca atgggacttc gtcgatcaac
    14401 cgatcattct taatcggctt ctcgggccgc acagcctgcg cgatccgggc gccaggctct
    14461 cgtggctgat tccgactccc ttttacaccg agctgatgct gggcgtgttc cacggccagg
    14521 gacactcggc cttcagcttc cggtcgcacg actacgacgg gactcacgga cgcacctcgc
    14581 tcgaacgcga gttgcacggg ccgggcgacc tgctctacgt gccgcgcatc accagttcct
    14641 tcgacctgag cgacacccag accctcgtcg ccggggcgtc cggcggcttt ggccccaaca
    14701 attccggcgc cgacgcggac tcgcaaatct acgggctgga tctctactgg aaatggaagc
    14761 cggtccacgc cgaacatgga ttcccattcc tcgcctggca gtccgaggga ttgtaccgcc
    14821 gctatgaggc aggggccgat tccgagcgcg acatgccttc ggaggacctc gacgactggg
    14881 gcttctactc ccagatcatg tgggggttca agcggcgatg ggtggccggt ctgcgcggcg
    14941 agtgggcgga tggcaatacc ggcgcctggg acgcaagcga tcgctttcga ggggagcgga
    15001 ttcgcttttc cccgaatctg acctgctatc cgactgcgtt ctcgaaaatc cggctccaat
    15061 acaacctgga tgacggagac cggttcggca ccgagcattc ggtctggctg caaggcgagt
    15121 tcctccttgg ctctcacggc gcccataaat tctgacatcc gttccgctat gaacagactc
    15181 gaccaaccgc ttgccaactc acgcgcccgc tggccctcgc tcctcctcgc cgcatgcgtt
    15241 ctgtcgtccc cgactgcctg ggccaagctc cgggtggtga caacgacgcc cgatttcgcc
    15301 gccatcgccg ccgagatcgg cggcgaccgc gtcgaggtca cgtcgctggg caaaccgacc
    15361 gaagacccgc acttcatcga cgcgcgacct agtttgattc tcaaattgaa ccgcgcggac
    15421 gtgctcgtgg agggtggggc ggaactcgag atcggctggc tgccgtcgct gatggacagc
    15481 gcgcgcaacc cgaagattca gcgcggcgcg cccggcaatg tctacgccag cgaagggatc
    15541 gagatgctcg aagtcccgtc cgtgctcgac cgctcgatgg gcggggtgca tccggtcgga
    15601 aacccggcgt tcatgaccga tccagtcaac gcccggattg tcgccgagcg tctggccagg
    15661 gtgttcggcc agctcgactc gaaatcgagc gctctgttca acgcaaacct gaaaagcttc
    15721 tccgacaaac tcgctggaaa gctgatcgag tggcggaatc gcctggcacc gttcaaaggc
    15781 agccggatcg tggcgtatca taactcgtgg ccctacttcg cccgccggtt cggacttcag
    15841 gtcgatctct tcctcgagcc gaagcccgga ctgccgccca cgccaaccca tttggcccag
    15901 gtcatggcca aaatgaagga gagcggcgca cgggtcatcc tcgtgcagcc ctatctcaac
    15961 cggcacacag cggagactgt cgccagccac acaggggccg tggtggtcga cgccgccggc
    16021 ttcccgggcg gcgtcaaggg gacggagggc gactactgca agttcatgga ccatctggtc
    16081 aattccttgg ccgatgcgtt gggaggcaag acaccgtcga cccagcaatg acgagcatgg
    16141 aaccaatcaa cattctggaa atgcttgtca ttctcaagtg gccgctgatc gcgagcctca
    16201 cgttgccctg gctgctggtc tatctgggcc tgcacgtcgt gcagcgcgag atcatcttcg
    16261 tcgacctcgc tctggcacaa gtggcggccc tgggcacatg cgtctcgctc ctgatggact
    16321 gccatctcca ttcctggcaa agctacgcct ggtcggccgc cttcaccgtg gttggcgccg
    16381 tggtcttcac gctcacccgc agccggcatc cgcgtgtgcc tcaggaggcg ctgattggga
    16441 ttgtctatgt cgtggcggcc gccgccggaa tcctgatcct catgcggagc accggcggca
    16501 aagaagagct gcaacgttcg ctcgttgggg agttgctggt tgtgcccccg cgcgaggtta
    16561 tccagaccat cggcttgtac ctgggcatcg gggcggtgca ttacgtcttt cgtcggaaat
    16621 tcctcgcgat ttccaaagac cccgacgccg cagtccgctc cgggctctcc gttcgagggt
    16681 gggatttcct cttctacgtg acctttggct tcgtggtcac tagcttcgtg catatcggag
    16741 gggtgctgtt gacattctcg tacctgatcg tgccggccgt atgcggcaat tacctggccc
    16801 acacgctgcg ccgacgcctg gcgatcggct ggaccgtcgc caccctcgcc agcctggcaa
    16861 gcctctgcct gacgccggcc ctcgacctgc ctgtcggggc cacgaccgta tgcatgctgg
    16921 ggcttctgct ggcaatcacg atggccctgg cgcgacgggt cccggcaccg caaacgcgtc
    16981 cggccgccgc ggacaccctg accgtcgccc gcagccagag cgacgcatga gaagagacaa
    17041 tgaatcagaa acaatactcg aaactcgatg tgactctggc cagcgtgtgc cgcagttgtc
    17101 cggtctgccg caccgcgcgc cggcgccgga ccggcttggc gtattggctg gtcaatcgag
    17161 tcgaaagctc tctctgcccg ttctgccggg cctacgaacg tgtttacggg cgcaagtccc
    17221 atgaaacggg cgccgcgcac caaccgcaat ccgacacatg atagaccgct gcacactctg
    17281 gttggcgaca gcgctgactc tggtctgcca ggccggcagt ctcgccgccg gcggcagccg
    17341 cgcccaggcg ctaaaggccc tgtgtggtcg gctcgagatc gggccaggcg cagtgattgc
    17401 cgacgtcggt tgcggcgagg gactcgacgc cgcggagttc gctcgagtcg tcggcgagag
    17461 cggcacggtg ttggcccagg agatcgaacc cgccaagctc gccacggtcg tccgggtggc
    17521 cgccaaacac ggcctgcatc agatcgttcc ggtcttggga cagagcgacg atccccgatt
    17581 gcccgatggt ttcgtcgacc tcatctacct gaaccgtgtg tttcatcact tggcccagcc
    17641 ccaggcaatg ctccggcgcc tccatcagga tcttaagccc ggcggtcttt tggtcgttgt
    17701 cgaccagcat agggggccgt tgaccgattg ggcgccgacc gagcgtcgcg aggaggagca
    17761 ccattggacc ggcgagacaa ctgtcgtccg cctggcacgt gaggcgggct tcctctttca
    17821 cgacgtgctc gaggacgtgt ggcacgaacc gcaaccgttc gtgctcgcat tccggaaacc
    17881 ggctgccccg ccgccggatg ccggcgatcc ggatttgccg cctccgctcg acgcgaaggc
    17941 cctggtccgt gcactgccaa tcccacagca agacggttgg gccgcgattt tcgtcggcct
    18001 ggaccgggga cgggccgtgc tgcccgctct gaaagagggg ctgccgccat ccacccggct
    18061 ggcggacgtg gtcttggacg agtgggctct gacgctggag gaactgccac ctggcgctcc
    18121 acaccccggc accgaggtgc tgagaaaaaa caacaacagc ctggccttgc cccccgacat
    18181 gcgtctgggc ctggtgctct tcgtcgacgc ctaccaatgg ctctgggaac cggtgccgct
    18241 gctccggcaa ctcagggaac gtatgccgga ctcagggctg ctcgccatcg tggaacgtca
    18301 gggcccggac gatgagccgc ggcgcttggc cgggcaccac cggcggatct cttcacggcg
    18361 ggtcgcagag gagatgcgcg aggctggctt tcaattgcgg caaaccctgg cgccgccgac
    18421 cgaggaccgc tgtttccttc tgtttgagcc gcggacctca cacgagaggc tgcagccgga
    18481 agccccgaag gagttgcgcc catgaaacct tgttcgaacg ataggcaaaa tcgcgctccc
    18541 aaaggaccgc agcactggta tcgtctgacc gggctccccg gctggaggcg ggcgcaactt
    18601 gggatgcctg ctttcggcga tccacactgc gtgccccatc ctcgccccaa tctcctgcga
    18661 cggttcatgg atgctctgcg acgacggttt gcgaggcaca cactcgagtc tgccgacccg
    18721 tctgaatcat ccccccaaaa tggtgaatcc tccaacaaca acacatgaaa atcgctatcc
    18781 ctctcgtagc cggacgcttg tctgagcact tcggccattg cgagcaattc gccctcatcg
    18841 aaaccgatct ggaatccaga caaatggtca gtcaaacccg catggttcca ccgccgcacg
    18901 cgccgggagt tttgccgcgg tggctccatg aacaaggggc gaaggtgatc attgccggcg
    18961 gcatgggccg gcgcgccctg gatttgttca ttcagaacgg catcgccgtc cataccggca
    19021 tcgcgggcgc ttcgcccgaa gagttggccc acgcattcct gacgggcgct ctgggcgaca
    19081 ccacgccgac ctgtggacat cggcggggcg atgagcgcca tcgccatcac ggatgccacc
    19141 acgacgccgg atcgagccac gaaccatgaa aatcgccatc tcaacacagg gaccccaacc
    19201 gaactcggcc ctcgaaccgc gtttcgggcg cgcgcgctat tttcggatcg tcgacactga
    19261 gaccgggcag cagaccgtag tggacaatga aaacgcggcc ggcgccgtcc aaggcgccgg
    19321 ccccagggcg gtccagatgc tggcgcgcct gggcgtccaa gccgttctga ccggccatgt
    19381 cggtcccaag gcatggaccg cgctcgaatc gagcaagatt caggcgta
//
LOCUS       DAOQRO010000012        18773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2065_length_18773_cov_5.436211, whole genome shotgun sequence.
ACCESSION   DAOQRO010000012 DAOQRO010000000
VERSION     DAOQRO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_18773_cov_5.436211"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKK20_00990"
     CDS             <1..221
                     /locus_tag="PKK20_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98498.1"
                     /translation="LRVEIQNPEGNRLPGFALNDCPEMIGDQIDRIVKWTRGSDLSAL
                     AGRPVRLKFALKDADLYAFRFRPAQPFQ"
     gene            complement(230..1690)
                     /locus_tag="PKK20_00995"
     CDS             complement(230..1690)
                     /locus_tag="PKK20_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNU98499.1"
                     /translation="MKPRMFLSRRAVIARLSAAGAALAGVAAARAAETDQPAPTRRAA
                     ARSGNARQLIAERVNQTPLIDTHEHLIDEPERLRGAGQAGVRCDDWALLLSHYLDSDL
                     LTAGMPRSDLDRFLSAEVEPAAKWTLLAPYWPRVKHTGYAQAVRIAIRELYGVDDLSA
                     ATVERVQAEYEKTRCAGFYERILRDRANIESCQVNCIFGAPFKESVSPTLLMQDLSIV
                     GMFAGPSIEQYGRPSGIEVKALSDWHRVIDWWFDRYAKYAVAVKSQDAYSRDIDYARV
                     PPEQAAPVFKRVLDKETVTAEERKLMQDHLFWYAVDQATRHGLPVKLHTGYYAGQSWM
                     PLERLARNAASAGELCRLSRDTQFVFMHICYPFYEDLLAVAKHWSNAWVDMCWAWIIN
                     PIAAKDYVKKHLVTAPANKLLPFGGDYIPVEPVLGHAALARRGLARALWELVGEGWLS
                     LDEALDLVEPILRGNARRLFQIDKKTEALRQAPWVR"
     gene            1798..3060
                     /gene="larA"
                     /locus_tag="PKK20_01000"
     CDS             1798..3060
                     /gene="larA"
                     /locus_tag="PKK20_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HNU98500.1"
                     /translation="MNVNLAYGQGNLTVDLPDNRTTVITPAHRPGLPDERAAVLGALR
                     QPIGCLPLSQWLRPSARVCVVFTDMTRATPNDRLIPWLLDELDGIPRDQITLLNATGT
                     HRPNTPEELERMLGSAVTRNYRVVNHECENTAMLVQCGKTCDGTPALLNRHLVEADVR
                     IVTGFIEPHFFAGFSGGPKGIMPGVAGLATVMSNHGARNIGDPQATFGITEGNPLWED
                     FRRIALRVGPSFLLNVALNEQRQITGVFAGDLIAAHQIGCEFVRQSAMQRVEAPFDIV
                     VTTNSGYPLDLNLYQGVKGLSAGARIVKTGGTLILACECREGIPSNSPLDRLLRSASS
                     PEAILTMLATPGPVRAEQWQAQIQALIQRRASVLLYSSLSDELTRAAFFNPCRDIGAT
                     VAQKLAELGPDARVAVLPQGPLTIPYLA"
     gene            3100..4455
                     /locus_tag="PKK20_01005"
     CDS             3100..4455
                     /locus_tag="PKK20_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98501.1"
                     /translation="MNRRTFLQTAGLGAAGLVFAPRSTYAEEFADLKKRVGLIGTGWY
                     GKCDLLRLIQVAPVEVVSLCDVDKRMVAEAAEIVAGRQKSGRTPRTYGDYRDMLKEKD
                     LDIVLVGTPDHWHALASIAAMNAGADVWVQKPISVDVAEGQAMLATARKLNRVVQVGT
                     QRRSTPHLIEARDRVLREGLLGKISHVDICCYWHMRARGNPPDIAPPEHLDYELWTGP
                     APMRPFCQWTHPRGWRAFMEYGNGIVGDMCIHMYDMVRWMLGLGWPRSISSSGGILVD
                     KASKANISDTQTAIFDHGDLQVVWTHRTWGDAVDPKYPWSATFYGDKGTLKTSVFSYD
                     FFPRDQDKAAAHGEPLYEYDKYPEDQTEKDLEKHCASAIRWHMRDFLAAIVSRGRPVA
                     DIEQGHISSASSIMANLSMQLGRSIAWDPALQQVAGDAEANRLLRRPYRAPWMHPEVE
                     S"
     gene            4541..5542
                     /locus_tag="PKK20_01010"
     CDS             4541..5542
                     /locus_tag="PKK20_01010"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNU98502.1"
                     /translation="MNRSPIRVAVTGAAGQIGYALLFRIASGALFGPKQPVILHLIEI
                     EPALSALEGVVMELDDCAFPLLKGVVPTADLDNGFRNVDWALLVGSVPRKQGMERKDL
                     IGINGRIFVGQGRAIQKNAASDVRVLVVGNPCNTNCLICMRNAPDVPPNRFFAMTRLD
                     ENRAKSQLARKAGVDVTAVTNMTIWGNHSATQYPDFQNARIGGRPATQVIADDAWLKG
                     EFIASVQQRGAAIIKTRGASSAASAANAAIDTVRSLLEPTPPGDWHSVCVCSDGSYGV
                     EPGLISSFPVRVQDGNWDIVPGVSLDEFGRSRLDITIRELEEERAMVAGLFNGVASK"
     gene            complement(5664..6410)
                     /locus_tag="PKK20_01015"
     CDS             complement(5664..6410)
                     /locus_tag="PKK20_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98503.1"
                     /translation="MRIESLNVNAEWASPDQTSSDAWHFLQDGPHPGAYNMALDEALL
                     EAAPTLGRPLLRFYEWSEPAATFGYSQSYAEVARLTPLRPLIRRPTGGGLVPHERDYT
                     YTVVVPPMHDWYRLRARESYRRVHDWLRRAFDRLGWRTDLAAAARTGAPGQCFVRAEQ
                     FDVLRDGAKIAGAAQRRTRNGLLIQGSIQPPPGAPARRAWRAAMRQAAAEDWSVRWSP
                     FAVPAALRRRAEQLATEKYGHPGLSKCGRI"
     gene            complement(6454..7161)
                     /gene="nth"
                     /locus_tag="PKK20_01020"
     CDS             complement(6454..7161)
                     /gene="nth"
                     /locus_tag="PKK20_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNU98504.1"
                     /translation="MARESHAARQERLVHIVERLRRTYPDADCELNRSNPLELLVATI
                     LSAQCTDKMVNRVTESLFRKYRTAADYAGASAEEFENDIRSLGFFRNKARSIRACCEK
                     LVVRHGGRVPQTMDELTQLDGVGRKTANVILGNAFGVNAGVVVDTHVTRLAGRLGLTA
                     QTQPEKIERDLMKLVPQKDWTLFSHWLIWHGRRRCKARKPDCAGCELSAWCPSAGRIA
                     KGARRPGAPRKRVASAS"
     gene            7331..9472
                     /gene="glgP"
                     /locus_tag="PKK20_01025"
     CDS             7331..9472
                     /gene="glgP"
                     /locus_tag="PKK20_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNU98505.1"
                     /translation="MTQKPFTANEINEMAVGLNRLARNLWWTWDQEAQDLFKELSPRG
                     WRNLYHNAVAVLHEVSDYELRVRLQDRRFAQHVQFVLRNFDQYMRSTDTWGHTNAAEL
                     RERPVAYFSAEFGFHETLPIAAGGLGVLAGDHAKSASDLGIGFVGISLFYREGYFMQA
                     INQDNWQTEYYTRLHPKNLPIEPVLDPKGDPLVSSVSIATSQVLFKAWRVNVGRNPVY
                     LLDTDLPENEQLYRDLTMHVYGGDTATRIMQEILLGVGGVRLLRALGVQPSVYHMNEG
                     HAAFLIFELLRERMAEGMTFANALAATREECLFTTHTPVEAGHDRFSNDLMHFTMHRL
                     FRHIRTPAAELMGLGRIRPDDEHEPFCMTVLALKASRAANAVSALHGEVSRAMWHPLY
                     PDRPVDKVPIGHITNGVHLPGWMKGTVRTFWKRKLGCDWEKGLNTPEFWTRVLDPEFI
                     SDEEIWALRHKLKREMIEFSRRHLLLQGERLGPGDFIAFDRLLSVDALTIGFARRFAT
                     YKRAPLIFQQFERIVELVKDRARPIQFVFAGKAHPRDDEGKKLIQRIIHLSRFSELSG
                     HLIFITNYDIHVARQMVSGCDVWLNNPRRPLEASGTSGMKTCTHGCLNLSILDGWWRE
                     GYDGTNGFAIGDDSHPDSVEEQDRRDSENLFQVLTEEIIPCYYNRSANGIPRAWIQKI
                     RRAMATLTPQFNTWRMVQEYTQKYYLRPAKQ"
     gene            9476..10573
                     /gene="rlmN"
                     /locus_tag="PKK20_01030"
     CDS             9476..10573
                     /gene="rlmN"
                     /locus_tag="PKK20_01030"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNU98506.1"
                     /translation="MPLCPIKSQSLENLQAQLHDWGEPAYRATQVVEWLYTRRAPGWD
                     AMTNLPRRLRERLAQHYAFTTLELVAQQGSADATRKFLWRLDDRALIESVLIPASPTA
                     PGADEETSRQTLCISTQVGCAYGCAFCASGLGGFKRNLGADEIVEQVLAAQRRSRVDN
                     LVVMGMGEPLANYDNLIRALRILNAPWGANIGARRITVSTSGLCPQIRRLAGEPQQFR
                     LAISLHGATDDVRNRLMPINHKHPLRDLIAACEAYQTIKGRLITLEYILISGVNDGLD
                     QTRPLAALARRLHAKVNLISYNRVDGLPWQRPSQPRQELFLAALQRHGVKATLRRERG
                     DDIDAACGQLRLRTEPHLAAPASAVDTPQSR"
     gene            complement(10680..12860)
                     /locus_tag="PKK20_01035"
     CDS             complement(10680..12860)
                     /locus_tag="PKK20_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU98507.1"
                     /translation="MNETIGAGRGAMPVRTSDAMRLGRSPAHLAAAGEPCQGDRSVPG
                     LASSAEAQLVEGVQTRGDRFFARIATEAPFTRMHPRVAAFFKDYLVKEKAVAFGDRFV
                     VNTHFPPYPSAAFDRLAEQFGEIGTAATRRLYSVTLAVTNRCPFRCWHCYNAGRSQQD
                     LPLAVLRDLAVQLEDRGAVMVTLTGGEPLLREDLPEIAAAFDPRSCLILGTTGEGLSP
                     ERAVELKRQGVFAVGISLDSHLAIEHDRLRGRPGAFESVLRALGVAREAGLYPYVVSV
                     ATREFLVRERFMPFLRFAAGAGACEVHLLEPSATGRLAGRAGVLLTAAERRRIFEYQA
                     EVAQDSTLPILSSFAYLESPEAFGCGAGLTHLYIDGSGEVCPCNLVPLSFGNVAREPL
                     DTILERMGRCFCRPRTVCVGRILAGRIPSTGLPLGPEESLAVCEQHLPQAHALPRFFR
                     IRDAAGDADVGAAELRKAYDRVRDDYDEFWVTAAGAPVERLIADLALRGGERVFEAGC
                     GTGFATAQLARHAGDVLAVDLSEGMLTRARKRLEESGVRNVRFQAGDALDALGAGGLF
                     DLIFSSWVLGYIPMASFLAAAHRALASGGRLAFIVHKERSPREPLEVFGELVAENPTV
                     LLKRVAFDFPRNADHVRTLVSAAGFGVQTVSESEVVFRYATARQVLDHLLKSGAGTAF
                     YDAIDPDRREALTERFLESLTHRQTPGAGYEVRHEYVACVAVKS"
     gene            complement(12928..15639)
                     /gene="aceE"
                     /locus_tag="PKK20_01040"
     CDS             complement(12928..15639)
                     /gene="aceE"
                     /locus_tag="PKK20_01040"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNU98508.1"
                     /translation="MDQQSSNTSASLTKGAGTAAGFTPTPEWLATHEQLLGMALANER
                     PDRVQRFLNGMLGRMRERGLDAPRSTSTPYVNTIAPDRQPALPGDRALEKRIKSYIRW
                     NAMAMVVNANRQHGGLGGHISTYASSATLYEVGFNHFFHGAGPDRAADMVYFQGHASP
                     GIYARAFLERRLDEHHLQHFRQELAEGGGLSSYPHPYLMPEFWQFPTVSMGLGPILSI
                     YQARFNRYLVARGLVRGREPKVWCFVGDGETDEPETLGSLTLAARENLDNLIWVVNCN
                     LQRLDGPVRGNGKIIQELEAAFRGAGWNVIKVIWGSDWDPLLAADKTGLLVKRMEEAL
                     DGDYQKYSVEPGSYTRRHFFGKYPELLELVNHLTDSQIHRLLRGGHDPVKVYAAYHAA
                     VTHRGAPSVILAKTIKGYGLGEAGEGRNISHQQKKMNEKELREFRARFAVPIRDEEIA
                     ETPFYRPPADSPETIYLLERRQALGGFVPERKVSAPPLEIPPREFYAEFTKGSGKMAA
                     STTMAFVRLLSLLLRHKGIGPRIVPIIPDEARTFGMDALFREIGIYSPKGQLYEPVDV
                     KSLLYYHESQKGQILEEGITEAGSMATFIAAGTAYATHGVNLVPFYTYYSMFGPQRIG
                     DLFWLAGDIKAKGFLLAATAGRTTLNGEGLQHQDGHSLLLTSTIPSLVTYDPAFAYEL
                     AVIVRDGMRRMYEEGEALCYYLCLYNENKVMPPMPEDVEDGILKGLYRFKPGVAGRRP
                     KAHIFGSGPILSEALRAQEILAERYKVSADVWSATSYKLLRHDALRARRWNMLHPTEP
                     RRKSYLESLLEKEKGVFVAVSDNMKAVPDMIAPWVPGGLTTLGTDGFGRSDTREKLRR
                     FFEIDAETTVVATLYSLAEKGTVGRHLVARAIKDLGVDPDKVYPQIV"
     gene            15829..17355
                     /locus_tag="PKK20_01045"
     CDS             15829..17355
                     /locus_tag="PKK20_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98509.1"
                     /translation="MNTGTPRRPWWGRLLVWTFRILILLAALLALRSWHAFRDRNPGY
                     TLALNVDPARSVVAPKPLHVGFGRVPINPNLSDPKRPVWLAGFSQNRAATALHDSLWA
                     HACVLDDGYTRLGIVSLDAIGLFHDDVIEVRRRLDPQWRLDYTIVCSTHNHSTPDLMG
                     LWGPHPLKTGVDPRYRAQVLTATTQALGQAIEALTPACVGFHEIQMPTDGLVRDSRKP
                     IVFDADLRAMHFTSPEGGGTLGTLVAWGNHPETVWSKNTEITSDFCGYLREALEKGVT
                     IDGQTHVAGLGGTHVYVTAALGGLMTTSPSVTVRDPYLDREFEKPSHDKARALGRQLA
                     SRLLPVLRTPNPAATNHAPIGVCARTIELPIDNAIFLLAPVLGILDRGHVRWRTMRTE
                     VALVTVGDASIACLPGEVYPEIVNGGIERAPGADFEIDPVEVPPIRALMPGKVKFVFG
                     LANDEIGYIIPKSQWDRKPPYLYDSDTPVYGEINSLGPETAPRLHAAFRALCGTSQSR
                     "
     gene            17456..17806
                     /locus_tag="PKK20_01050"
     CDS             17456..17806
                     /locus_tag="PKK20_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNU98510.1"
                     /translation="MRAYYRTFRPSVWLFARSRDPAQPLLERSGQRMFWDAVRRAGLS
                     RKGGIHSLRHWFARHLLEAGVEITAVQRLLGHANLSSTTTYLHVREERPAQIQGPLQQ
                     LNLPAMPAIESAAA"
     gene            18023..18724
                     /locus_tag="PKK20_01055"
     CDS             18023..18724
                     /locus_tag="PKK20_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98511.1"
                     /translation="MIDTSFNVYSDARGGDPDATSPTLRAYHKFLWSKPLPSGDRFDL
                     RDDEPGYYLYHDSALGNFALGSDAITHSYKTHIKKKWLTTQIPKEVQELFDRGSTIGA
                     YIVFPNNQVDRKHTINQARGVSRLIDDRFDLTLECIRRFYIGEPNPLGESLNRYAAFF
                     QLFETFKGYVEFFLLDDLVNERGDVKFYLPFDDFQSPPEFKDVKDYLVYKNRVESFIK
                     SRNRRIEDYATRTKT"
BASE COUNT         3535 a         6436 c         5539 g         3263 t
ORIGIN      
        1 ttctccgtgt ggaaatccag aatcccgagg gcaatcgcct tcccggattc gccctgaacg
       61 actgccccga gatgatcggc gatcagatcg accggattgt gaagtggacg cgcggcagcg
      121 atctcagcgc gcttgccgga cgccccgtcc gcctcaaatt cgcgttgaag gacgccgatc
      181 tctacgcgtt ccggtttcgt ccagcgcagc cgttccaata agcggcccct caacgcaccc
      241 agggtgcctg gcgcaaggcc tcggtcttct tatcgatctg gaagagccgg cgcgcattcc
      301 cgcggagaat gggctcgacc agatcgagag cctcgtccaa agacagccag ccttcaccga
      361 ccagttccca taaagcacgc gcgaggccgc ggcgcgccag agcggcgtgt ccgagcaccg
      421 gctcgacggg gatgtaatcg ccgccaaagg gaagcagctt gttggccggc gcggtcacaa
      481 ggtgtttctt gacgtaatcc ttcgcggcga tggggttgat gatccaggcc cagcacatgt
      541 cgacccaggc attggaccag tgcttggcca cggcgagcag gtcttcgtag aagggatagc
      601 aaatgtgcat gaagacgaat tgcgtgtcgc gggacagccg gcacaattcg cccgccgacg
      661 ccgcgttccg cgccaagcgc tccagcggca tccagctctg cccggcatag tagccggtgt
      721 gcagtttcac cggcagaccg tgccgcgtcg cctgatccac tgcataccag aacaggtgat
      781 cctgcatgag cttgcgctcc tcggcggtga ccgtctcctt gtcgagcacg cgcttgaaga
      841 ccggggcggc ctgctccggc ggcacacgcg cgtaatcgat gtcgcggctg taggcatcct
      901 gcgacttcac cgccaccgcg tacttcgcgt agcggtcaaa ccaccagtcg atgaccctgt
      961 gccagtcgga caacgccttc acctcgatcc ccgaaggccg cccgtattgc tcgatactgg
     1021 ggccggcgaa catgcccacg atgctcaagt cctgcatcag cagagtcggg gagaccgatt
     1081 ccttgaatgg cgctccgaaa atgcagttga cctggcacga ctcgatgttt gcgcggtcgc
     1141 ggagaatgcg ctcatagaaa ccggcgcagc gcgtcttctc gtattcggcc tggactctct
     1201 cgacagtcgc cgcggatagg tcgtccaccc cgtacaactc gcgaattgcg atgcgaaccg
     1261 cctgggcgta accggtatgc ttgacccgcg gccagtatgg cgcaagaagc gtccacttgg
     1321 cggccggctc gacctccgcc gagagaaacc ggtctagatc cgaccgcggc atgccggccg
     1381 tgagcaggtc cgaatcgagg taatgactca acaacaacgc ccagtcatca caccgtactc
     1441 cggcttgtcc ggcgccccgc aaacgttccg gttcgtcaat caggtgttcg tgggtgtcaa
     1501 tgaggggcgt ttgattcact cgttcggcaa tgagctggcg ggcattgccg gagcgggcag
     1561 cggcgcggcg ggtaggcgcg ggctgatccg tttccgcggc ccgggcggcg gcgacgcccg
     1621 ccagcgctgc gccggcggcg ctcaatcggg cgatcacagc acgacgggag aggaacatgc
     1681 gtggcttcat agtgggtcgc agcatgcact ccagccgcct gccgcgcaag cccgccgccg
     1741 cctttcgcct tggcactgcg ccctggcgcc gtttaaatcg catccgcctt tgctgccatg
     1801 aacgtcaacc tcgcctatgg tcagggaaac ctgacagtgg atctcccaga caatcgcacc
     1861 acagtgatca caccggccca tcgcccgggt ctgcccgacg agcgggccgc cgtgctgggc
     1921 gcgctgcgcc aaccgatcgg gtgcctgccc ctcagccagt ggctacgacc ctcggcccgg
     1981 gtttgtgttg tgttcacgga catgacgcgc gccacaccga acgatcggct catcccttgg
     2041 cttctcgacg agctggatgg gattccgcgc gaccagatca cgctcctgaa cgcaacgggc
     2101 acgcaccggc ccaacacgcc cgaggaactc gagcgcatgc tcggctccgc ggtgacgcga
     2161 aactaccgcg tcgtcaatca cgagtgcgag aacaccgcga tgctggtcca atgtggcaaa
     2221 acgtgcgacg gcacccccgc cctgctcaat cgacacctcg tcgaggcgga cgtgcggatc
     2281 gtcaccggct tcatcgagcc ccatttcttc gccggattca gcggcggacc caagggcatc
     2341 atgcccggcg tcgcaggcct ggcgaccgtc atgagcaacc atggcgccag aaacatcggc
     2401 gatccccagg ccaccttcgg catcacggaa ggcaatcccc tgtgggagga cttccgccgc
     2461 attgcgcttc gggtcggccc gagcttcctc ctgaacgtcg cgctgaacga acagcgccag
     2521 atcaccggcg tgttcgccgg cgatctcatc gccgcgcacc agatcggctg cgagttcgtc
     2581 cggcaatccg ccatgcagcg tgtcgaggca ccgttcgaca tcgtcgtcac caccaacagc
     2641 ggctaccccc tcgatctcaa cctctatcaa ggcgtcaaag gtctcagcgc cggggccagg
     2701 attgtgaaga cgggcggcac tctcatcctg gcctgcgaat gccgcgaggg cattccctcg
     2761 aacagccccc tggaccgcct cttgcgcagc gcgtcgagtc cggaggcgat cctgaccatg
     2821 ctggcgacgc ccgggcccgt gcgcgccgaa cagtggcagg cccagatcca ggccctgatc
     2881 cagcgccggg cttcggtcct cctttacagc tcgctctccg acgaactcac acgggccgcc
     2941 ttcttcaacc cgtgccgcga catcggcgcc actgtggcac aaaaactcgc cgaactcggc
     3001 ccggacgccc gggtcgccgt cctgccccag ggaccgctga cgattcctta ccttgcataa
     3061 gcttgtcacg acgcccggct ttcgactaga aacacagcta tgaaccgccg cacattcctc
     3121 caaaccgccg ggctcggcgc cgccggtctc gtgttcgcac cccgcagcac ctacgccgag
     3181 gaattcgccg acctcaagaa gcgcgtgggc ctcatcggca ccggctggta cggcaaatgc
     3241 gatttgctcc gcctcatcca ggtcgcgccg gtcgaagtgg tgtcgctgtg cgacgtcgac
     3301 aaacggatgg tggcggaggc ggcggagatc gtggccgggc gccagaaatc cgggcgtacg
     3361 ccccgcacct acggcgacta ccgggacatg ctcaaagaaa aggacctcga catcgtgctc
     3421 gtcgggacgc ccgaccattg gcacgccctg gccagcatcg ccgccatgaa cgccggcgcc
     3481 gatgtttggg tgcaaaagcc gatcagcgtc gatgtcgccg aaggccaggc catgctcgcc
     3541 accgcccgca aactcaacag agtcgtccag gtcggcactc agcggcgcag cacgccccac
     3601 ctcattgaag cgcgcgaccg tgtcctccgc gaaggtctcc tgggcaagat cagtcacgtc
     3661 gatatctgct gctactggca catgcgcgcc cgcggcaacc cgcccgacat cgcgcccccc
     3721 gagcatctcg attacgaact gtggaccggc ccggccccaa tgcgcccgtt ctgtcaatgg
     3781 acccatccgc gcggctggcg cgccttcatg gagtacggca acggcatcgt cggcgacatg
     3841 tgcatccaca tgtacgacat ggtgcgttgg atgctcggcc tgggctggcc gcggagcatc
     3901 agttcctccg gcggcatcct cgtggacaaa gccagcaagg ccaatatctc cgacacccag
     3961 accgcgatct tcgaccacgg cgacctccag gtcgtctgga cccatcgcac ctggggcgac
     4021 gccgtcgacc ccaagtaccc ctggagcgcc acattctatg gcgacaaagg caccctcaag
     4081 accagcgtct tcagctacga cttcttcccc cgagaccagg acaaagccgc tgcccacggc
     4141 gaaccactct acgaatacga caagtatccc gaggaccaga ccgagaaaga cctcgaaaaa
     4201 cactgcgcct ctgccatccg ttggcatatg cgcgactttc tcgccgcgat tgtctcgcgc
     4261 gggcgccccg tagccgacat cgagcagggg catatctcga gcgcttcaag catcatggcc
     4321 aacctctcga tgcaattggg ccggtccatc gcatgggacc ccgcgctcca acaagtggca
     4381 ggcgatgccg aagccaaccg cctcctgcgg cgcccgtacc gcgctccgtg gatgcacccg
     4441 gaagttgaga gttgagagtt ggaagttgga agttggaacc cgaacacgct ctgtttccca
     4501 acttccaacc gattgcctgt cgaatgattc ctgttttcgc atgaaccgtt cccccatccg
     4561 tgtcgccgtc accggcgccg ccgggcagat cggatacgcc ctcttgttcc gcatcgcctc
     4621 cggggccctc ttcggtccca aacaacctgt catcctccat ttgattgaaa tcgagcccgc
     4681 cttgagcgcg ctcgagggcg tggtcatgga actggacgac tgcgccttcc ccctgctcaa
     4741 aggggtcgtt cccaccgcgg acctcgataa cggcttccgg aacgtcgatt gggccttgct
     4801 ggtcggcagc gtgccgcgca agcagggcat ggaacgtaaa gacctcatcg gcatcaatgg
     4861 acgcatcttc gtcggccagg gccgggccat ccagaaaaac gccgcctccg atgtccgcgt
     4921 ccttgtggtc ggcaacccct gcaacaccaa ttgcctcatt tgcatgcgca atgccccgga
     4981 cgtgccgccc aaccgcttct tcgccatgac ccgcctcgac gagaaccgcg cgaagtcgca
     5041 actggcgcgc aaggccggcg tcgatgtcac cgccgtcacc aacatgacga tctggggcaa
     5101 tcactccgcg acccagtacc cggatttcca aaacgcccgg atcggcggca gaccggcaac
     5161 ccaggtgatc gcggacgatg cctggttgaa aggtgagttc atagccagtg tccaacagcg
     5221 cggcgccgcc atcatcaaga cccggggtgc ctcgagtgcc gccagcgccg caaacgccgc
     5281 catcgacaca gtccgctccc tgctcgaacc gacaccgccg ggagactggc acagcgtttg
     5341 tgtctgctcg gacggcagct acggcgtcga gcccggcctg atcagctcct tcccggtccg
     5401 cgtccaggac gggaactggg acatcgtccc gggagtgtct ctcgacgaat tcggtcgatc
     5461 gaggctcgat atcacgatcc gcgaactcga agaagaacgc gcgatggtcg ccggcctgtt
     5521 caatggggtc gcatctaaat agttgacaat tagtgcaggg ctctgctcca ggcctatggc
     5581 gcgcgccacg gaggagcgct gttcccggat catgcggagc acaaacgcgc tgatgcgaag
     5641 ctcgggagtg tattgtcgac tgtttagatg cgaccgcatt tagaaagacc ggggtggccg
     5701 tacttctcgg tcgcgagctg ttcggcccgt cggcgcaggg ccgccggcac cgcgaacgga
     5761 ctccaccgca cagaccagtc ttccgccgcc gcctgccgca tcgccgcccg ccacgcgcgc
     5821 cgcgccgggg cgccgggcgg cggctggatc gagccttgaa tcaagagacc gttgcgggtg
     5881 cggcgctgcg ccgcaccggc gatcttggca ccgtcgcgca gcacgtcgaa ctgctcggcg
     5941 cgaacgaagc attgaccggg cgctccagtg cgggcggcgg cggccaggtc tgtccgccat
     6001 ccaagccggt caaaggcgcg ccgcagccaa tcatggacac gccggtagct ctcccgggcc
     6061 cgcagtcggt accagtcgtg catgggcggc acgacaaccg tgtaggtgta atcgcgctcg
     6121 tgcggcacga gtccgccgcc ggtcgggcgg cggatgagcg ggcggagcgg tgtcaggcgc
     6181 gccacctcgg cgtaggactg ggaatagccg aatgtcgcgg ccggctccga ccattcgtag
     6241 aagcgcaaca acggacgccc aagtgtcggc gccgcctcga gaagggcctc gtccaacgcc
     6301 atgttgtatg cgcccggatg cgggccgtct tgcaggaagt gccaggcgtc agaagaggtc
     6361 tggtcggggc tggcccattc agcgttgaca ttcaaggatt caatgcgcat ggggaccatg
     6421 aacctgaaac gttcaacgcc cacctctcga ctctcaactt gctgacgcta cccgttttcg
     6481 cggcgctccg ggccgacgcg cgcccttcgc gattcggccg gctgaggggc accacgcgga
     6541 cagttcgcat ccggcgcaat cgggtttgcg cgccttgcag cgccggcgcc cgtgccagat
     6601 gagccagtga ctgaagaggg tccagtcttt ctgcggcacc agtttcatga ggtcccgctc
     6661 gattttctcg ggttgcgttt gagccgtcag acccaaccgc ccggccagac gcgtcacgtg
     6721 ggtatcgacc accacgccgg cgttgacgcc gaacgcgttt cccagaatga cattcgcggt
     6781 cttgcggccc accccatcga gttgcgtcag ctcgtccatc gtttgcggca cgcgcccccc
     6841 atggcggacg acgagttttt cgcagcaggc gcggatgctc ctggccttgt tgcgaaagaa
     6901 acccaggctg cgaatgtcgt tctcgaactc ttcagcgctg gcgccggcgt aatcggcggc
     6961 ggtcctgtac ttccggaaga gcgattcggt cacccgattc accatcttgt cggtgcattg
     7021 ggccgagaga atggtggcga ccagcagttc gagtggattc gagcgattga gttcgcagtc
     7081 cgcatcggga tatgtgcggc gcaagcgctc aacgatgtgg accaaccgct cttggcgcgc
     7141 ggcgtgcgat tctcgggcca taaacgtcgc ccgcgaccat agggaaccga ttcggtcggg
     7201 ggaagcgcaa aatgccaaat ccgggcgcgt ggaaggcaca aaatggtttg cgatggaggc
     7261 cggtggtgtc tagcgttcgc gcactttggc tgtcgacctg ggtgcagggc ggcgccggca
     7321 caactccgat atgacgcaga aaccatttac ggcgaacgaa atcaatgaaa tggcggtcgg
     7381 actcaaccgc cttgcccgaa atctttggtg gacctgggat caagaagccc aggacctctt
     7441 taaggaactg tccccgcgcg gctggcgcaa tctctaccac aatgccgtcg ccgttctgca
     7501 cgaagtctcc gactacgagt tgcgggtgcg cctgcaggat cggagatttg cccagcacgt
     7561 gcaattcgtg ttgcgcaatt tcgaccagta catgcggagc acggacacct ggggccacac
     7621 gaacgccgcc gaactgcggg agcgtccggt ggcgtatttc agcgcggagt ttggctttca
     7681 cgagaccctg ccgatcgccg caggcggttt gggcgttctg gctggcgacc acgccaagtc
     7741 cgccagcgac ctcggcatcg ggttcgtcgg catcagcctc ttctatcggg aggggtattt
     7801 catgcaggcg atcaaccagg acaactggca gacggagtac tacacgcggc tccaccccaa
     7861 gaaccttccg atcgagccgg tcctcgatcc caagggcgac ccgctggtct cttcggtcag
     7921 catcgccacg agccaggttc tgttcaaggc ctggcgagtc aacgtgggcc gcaatcccgt
     7981 ctacctgctc gacaccgatc tgcccgagaa cgagcagctc taccgggacc tgaccatgca
     8041 cgtctacggg ggtgacaccg ccacgcgcat catgcaggaa atcctgctcg gcgtgggcgg
     8101 agtccgactg ctgcgcgccc tgggggtgca gccttcggtt taccacatga acgagggcca
     8161 cgccgcgttt ctgatctttg aactgctccg cgagcggatg gccgaaggga tgacattcgc
     8221 caacgcgctg gccgccacgc gtgaggagtg cctgttcacc acccacacgc cggtcgaggc
     8281 ggggcacgat cgcttcagca acgacctcat gcatttcacc atgcaccgcc tgttccgcca
     8341 catcaggact cccgccgccg agttgatggg gctcggacgc atccggcccg atgacgagca
     8401 cgagccgttc tgcatgaccg tcctggcgct caaggcctct cgcgccgcca acgccgtcag
     8461 cgcgctgcac ggcgaagtca gccgcgcgat gtggcatcct ctctaccccg accgccccgt
     8521 cgacaaagtc ccgatcggcc acatcaccaa cggcgtccac ctgcccggct ggatgaaggg
     8581 cacggtgcgg accttctgga agagaaagct cgggtgcgac tgggagaagg ggctcaacac
     8641 gcctgagttc tggacgcgcg tgctcgatcc ggagttcatc agcgacgagg aaatctgggc
     8701 tctgcgccac aagctcaagc gcgagatgat cgagttcagc cgccgccacc tcctgctcca
     8761 gggagaacgc ctcggtcccg gcgatttcat cgccttcgat cgcctgctca gcgtcgacgc
     8821 cttgacgatc ggcttcgccc gccgcttcgc cacctataag cgcgccccgc ttatcttcca
     8881 gcagttcgag cgcatcgtcg aactggtcaa ggaccgggcg cgtccgatcc agttcgtctt
     8941 cgccggcaag gcccatccgc gcgatgacga gggcaagaag ctcattcagc gcatcattca
     9001 cctgagccgg ttcagcgagt tgagcgggca cctgatcttt atcaccaatt acgacatcca
     9061 tgtcgcccgg caaatggtgt ccggttgcga tgtctggctc aacaacccgc gtcgcccgct
     9121 cgaagccagc ggcaccagcg gcatgaagac atgcacccac ggctgcctga acctgagcat
     9181 cctcgatggc tggtggcgcg aaggctacga cggcaccaac ggattcgcca tcggcgacga
     9241 ttctcatccc gacagtgtcg aggagcagga ccggcgcgac agcgagaatc tcttccaggt
     9301 cctgaccgag gaaatcattc cctgctacta caaccgctcg gccaacggaa tcccgcgcgc
     9361 gtggattcag aaaatccgcc gcgccatggc caccctcacg ccccagttca acacctggcg
     9421 catggtgcag gagtacactc aaaagtacta cctgcgcccc gcgaaacagt gatccgtgcc
     9481 gctctgcccc atcaagtcgc aatcgctcga gaatctccag gcccaactgc atgactgggg
     9541 cgagcccgcc taccgcgcca cccaggtggt cgagtggctc tacacccggc gggcgccggg
     9601 ctgggacgcc atgaccaacc tgccgcggcg cctgcgcgag cgcctggcgc aacactacgc
     9661 gttcaccacg ctcgaactcg tcgcccagca gggatcagcc gacgccactc ggaaattcct
     9721 gtggcgcctc gatgatcgag ccctcatcga gagcgttctc atccccgcca gcccaaccgc
     9781 tcccggtgcc gacgaggaga ccagccgcca aacgctctgc atctccaccc aggtcggatg
     9841 cgcgtacgga tgcgcgttct gcgccagcgg gctcggcggc ttcaaacgca acctcggcgc
     9901 cgacgagatc gtcgagcagg tcctggccgc ccaacgccgc agccgcgttg acaacctcgt
     9961 cgtcatgggc atgggcgagc cgctggccaa ctacgacaac ctcatccgcg ccctccgcat
    10021 cctcaatgcg ccctggggcg ccaacatcgg cgcccgcaga atcactgtgt ccaccagcgg
    10081 cctctgcccc cagattcgac gcctggccgg cgagccacag cagttccggc tcgccatctc
    10141 cctccacggc gccaccgacg acgtgcgcaa tcgcctgatg ccgatcaacc acaagcaccc
    10201 gctccgcgat ctcatcgccg cctgtgaggc ctatcagacg atcaaaggac gcctcatcac
    10261 cctcgagtac attctcatca gtggagtcaa tgacggcctc gaccagaccc ggcctctcgc
    10321 cgccctcgcc cgacgccttc acgcaaaggt caatctcatc tcgtacaatc gcgtcgacgg
    10381 tctcccttgg caaaggccct cccaaccccg ccaggaactc ttcctcgccg ccctccaaag
    10441 gcatggcgtc aaggccacgc tccgccgcga gcgcggtgac gacatcgacg ccgcctgcgg
    10501 tcaactccgg ctccgcaccg aaccgcacct ggccgcgccg gcctccgccg tcgacacgcc
    10561 ccaatcgcgc tgatcccggc gcgccgttcg atttcctctg gcgcgcatga gtgcgcctct
    10621 cgatgacatc ggctccgcgt tcttcaaaag tcggtgagaa atgcgcgcca gaccgaatgt
    10681 cacgatttga cggccacgca cgccacatac tcgtgccgga cctcgtaccc ggccccaggc
    10741 gtttggcggt gcgtgaggct ctcgagaaaa cgctccgtga gagcctcacg acgatctggg
    10801 tcaatagcat cgtaaaaggc ggttccggcg cccgacttga gtagatgatc aagcacctgc
    10861 cgcgccgtcg cataccgaaa gacaacctcg ctctcgctga cggtttgaac cccgaacccg
    10921 gccgccgaca cgagtgttcg aacgtggtcc gcgttgcgcg ggaaatcgaa cgcaacccgc
    10981 ttgagaagga cggtcggatt ctcagccacg agttcgccaa agacctcgag cggctctcgc
    11041 ggcgatcgct ccttgtgcac aatgaacgcc aggcgcccgc cagacgccag ggcccgatgc
    11101 gccgccgcga gaaatgacgc catcgggatg taacccagga cccaactcga aaagatcagg
    11161 tcgaacagcc cgcccgcgcc cagagcatcg agtgcgtccc cggcctgaaa ccggacgttt
    11221 cggacacccg actcctcgag gcgctttcga gcgcgcgtca acatgccttc cgacaggtcg
    11281 acggccagca cgtctccggc gtggcgggcc agttgggccg tggcgaatcc cgtgccgcag
    11341 ccggcctcga acacacgttc gccgcccctg agcgccaggt ccgcaatcag acgttccacc
    11401 ggcgccccgg cggccgtcac ccaaaactcg tcgtaatcat cccgcacccg gtcatacgcc
    11461 ttgcgcaact ccgccgcccc cacgtccgca tcgcccgccg catcccggat tctgaagaag
    11521 cgcggcaggg catgggcctg cggcaaatgc tgttcgcaaa ccgccagaga ctcttcaggt
    11581 cccaggggca ggccggtcga cgggattcgc ccggccagga tgcggcccac acacacggtg
    11641 cgtggccggc aaaaacagcg ccccatgcgc tcgagtatcg tatcgagcgg ttcacgcgcg
    11701 acattgccaa aggaaagcgg caccaggttg cacggacaga cctcgccgct gccgtcgatg
    11761 taaagatgcg taagacccgc gccgcagccg aacgcctcgg gagactcgag gtatgcaaaa
    11821 ctcgacagga tcggcaacgt gctgtcctga gccacctccg cctggtattc gaaaatccgc
    11881 cggcgctccg ccgcggtcag cagcaccccg gcccgtccgg ccagccgacc cgtggcgctc
    11941 ggttcgagca gatgtacctc gcacgcgccg gcccccgcgg caaaccgcaa gaagggcatg
    12001 aagcgctccc gcacaagaaa ctcgcgggtc gccaccgata caacgtacgg ataaagcccc
    12061 gcctcccgcg ccacaccaag ggcgcgcagg acggactcga acgctcccgg ccggcccctg
    12121 agccggtcgt gctcgatcgc gaggtgcgag tcgaggctga tgcccacggc gaagaccccc
    12181 tggcgcttca actcgacggc gcgctcgggc gacaggccct cgcccgtagt ccccagaatc
    12241 aggcacgaac gcgggtcgaa cgccgcggcg atctcaggca gatcctcccg cagcaacggc
    12301 tcgccccccg tcagggtcac catgacggcg cctcggtcct ccagttggac tgccagatcg
    12361 cgcaacactg caagcggaag gtcctgctgg ctgcgccccg cattgtaaca gtgccagcac
    12421 cggaacggac agcgattggt caccgcaagc gtcaccgagt acaggcggcg cgtcgccgcg
    12481 gtgccgatct ccccgaactg ctcggccaac cgatcgaacg ccgcgctcgg atacggcggg
    12541 aaatgcgtgt tcaccacaaa gcgatccccg aacgccaccg ccttctcctt caccagatag
    12601 tccttgaaga acgccgcgac cctcggatgc atcctggtaa agggcgcttc cgtggctatc
    12661 ctggcaaaga accggtcgcc acgcgtctgg accccttcga caagctgcgc ctcagccgaa
    12721 gatgccaacc ccggcacgga tcgatcgccc tggcacggct ccccagccgc cgccagatgc
    12781 gccggtgacc gccccagccg catcgcatcg ctcgtccgca ccggcatcgc cccgcggccc
    12841 gcgccaatcg tctcgttcat ggcgcttttc tacgacccgt cgtcggggac cgctaattga
    12901 aaaaacaagc gccaacatcg actgggatca aacgatctgc ggatagacct tgtccgggtc
    12961 cacgcctagg tccttgatgg cgcgggcgac aaggtggcgt ccgaccgttc ccttctcggc
    13021 caggctgtag agcgtcgcca ccacagtcgt ctcggcgtcg atctcgaaga accggcgcag
    13081 tttctcgcgt gtgtcgctgc gtccgaatcc gtccgtgccc agcgtggtca gcccgcccgg
    13141 cacccatggg gcgatcatgt cgggcacggc cttcatgtta tccgacacgg ccacaaacac
    13201 gcccttctcc ttttcgagaa gggattcgag gtacgacttt ctgcgcggct cggtcgggtg
    13261 gagcatgttc caacgccggg cgcgcagggc gtcgtggcgc aggagcttgt agctggtcgc
    13321 gctccagaca tccgccgaca ccttgtagcg ttcggcgaga atctcctggg ctcggagcgc
    13381 ctcgctcagg atcgggccgc tgccgaagat gtgggccttg gggcggcgtc ccgcgactcc
    13441 gggcttgaag cgatagagcc ccttgagaat cccgtcctcg acgtcctcgg gcatgggggg
    13501 catcaccttg ttctcattgt ataaacagag gtaatagcaa agtgcctccc cctcctcgta
    13561 catccgccgc atcccgtccc gaacgatcac cgccagctcg taagcgaacg ccgggtcgta
    13621 ggtcaccagc gacgggatcg tgcttgtcag cagcaggctg tggccgtcct gatgctgcaa
    13681 tccttcgccg ttgagcgtcg tgcgtccggc cgttgccgcc aacagaaagc ccttggcctt
    13741 gatgtcgccg gccagccaga agagatcgcc gatccgctgc gggccgaaca tcgaatagta
    13801 tgtgtagaag gggaccagat tcaccccatg cgtggcgtag gctgttccgg cggcgatgaa
    13861 cgtggccatc gatccggcct cggtaatccc ctcctcgaga atctggcctt tctgagactc
    13921 gtggtaatac aacagcgatt tcacatcgac cggctcatag agctggcctt tgggcgagta
    13981 gatgccgatc tcgcggaaca gcgcgtccat gccaaaggtg cgggcctcgt caggaatgat
    14041 cggaacgatg cgcgggccga ttcctttgtg gcgcaacagg aggctgagca gacgcacgaa
    14101 ggccatggtg gtggacgcgg ccatcttgcc cgagcccttg gtgaactcag cgtagaactc
    14161 ccgaggcggg atttcgagcg gcggcgcgct gaccttgcgc tcgggaacaa agccgcccag
    14221 ggcctgccgg cgctcgagaa gatagatggt ctccggactg tcggccggcg ggcggtagaa
    14281 cggagtctcg gcgatctctt cgtcccgaat cgggacggcg aatcgggcgc ggaactcgcg
    14341 gagttccttc tcgttcattt tcttctgctg atgcgagatg tttcggccct cgcccgcctc
    14401 ccccagcccg tagcctttga tcgtcttggc cagaatgacg gaaggcgccc cgcgatgcgt
    14461 gaccgcggca tggtaggcgg cgtagacctt cactggatcg tgtcctccgc gcagcagacg
    14521 gtgaatctgg ctgtcggtca gatgattcac cagttcgagc agttcgggat acttgccgaa
    14581 gaagtgccgg cgtgtgtagc tgcccggttc gaccgagtac ttctggtaat cgccatcgag
    14641 cgcctcttcc atccgcttga ccagcagccc cgtcttgtcc gccgcaagca gcgggtccca
    14701 atccgatccc caaatcacct tgatgacatt ccaccccgcc ccccgaaacg ccgcttcgag
    14761 ctcctggatg atcttgccgt tgccgcgcac ggggccgtcc aggcgctgca gattgcagtt
    14821 caccacccag atgagattgt cgagattctc gcgagccgcc agcgtgagtg agccgagggt
    14881 ctcaggctcg tccgtctcgc cgtcgccgac aaaacaccag accttgggct cgcgcccccg
    14941 cacaagcccg cgcgccacga gatagcggtt gaaccgcgcc tggtagatcg acaggatcgg
    15001 ccccagcccc atcgacacgg tgggaaactg ccaaaactcc ggcatcaaat aaggatgcgg
    15061 atacgacgat aacccgccgc cctcggccag ttcctgccgg aaatgctgca gatgatgctc
    15121 atcgagacga cgttccagaa acgcccgcgc atagatgccg ggggacgcat gcccctgaaa
    15181 ataaaccatg tctgccgcgc ggtcgggacc ggcgccgtga aagaagtggt tgaacccgac
    15241 ttcgtagagc gttgcgctcg acgcgtaggt cgagatgtgc ccgcccaacc cgccgtgttg
    15301 gcggttggca ttgaccacca tggccatggc attccaccgg atgtagctct tgatcctctt
    15361 ctcgagcgcc cgatccccgg gcaacgccgg ctgacgatcc ggcgcaatcg tgttcacgta
    15421 aggcgtgctc gtggatcgag gcgcgtccaa accccgctcc cgcatgcgcc ccaacatccc
    15481 gttcagaaac cgctggacac gatccggacg ctcgttcgcg agggccatcc caagcagttg
    15541 ctcatgcgtg gccagccact ccggcgttgg ggtgaatccg gccgctgtcc ccgcgccttt
    15601 cgtcagtgag gcggacgtgt tggaagactg ctggtccata gtgttcacaa atccttgcca
    15661 cctaaaaccc aatgcaattg cgccgagttt gccccctgtc gctcgcttgt ttcaaggatt
    15721 ctcttggggt taccgttctc cgcgaagcgt gtgagatgag cgtatcgtcg acgcggactc
    15781 acgtccgcgg cttgccgccg ccgccgcttc cgtgtatgcc tgccgcccat gaacaccggc
    15841 actccccgtc gcccctggtg gggccgcctc ctggtgtgga cctttcgaat cctgatcctg
    15901 ctcgccgcgc tcctcgccct gcggtcctgg cacgccttcc gcgaccggaa tccaggctac
    15961 acgctcgcgc tgaacgtcga ccccgcccgt tccgtcgtcg cgccgaaacc tctacacgtt
    16021 ggcttcggac gcgtcccgat caaccccaat ctctcggacc caaaacgccc ggtctggctg
    16081 gccggattca gtcagaaccg cgccgccacg gcgctgcacg acagcctctg ggcccatgcc
    16141 tgcgtgctcg acgacggcta cacgcgcctg ggcatcgtgt cgctcgatgc catcggcctg
    16201 ttccacgacg atgtcatcga ggtgcggcgc cgtctcgatc cgcaatggcg cctcgattac
    16261 acaatcgtct gctcgactca caaccactcg acacccgatc tcatgggcct gtggggaccg
    16321 catccgctca agaccggcgt cgacccgcgg tatcgcgctc aagtcctgac ggccacgacc
    16381 caggctttgg gccaggccat cgaagccctc acacccgcat gcgtcggctt tcacgaaatc
    16441 caaatgccca ccgacggcct agtccgcgac tcgcgcaaac cgatcgtctt tgacgccgac
    16501 ctgcgcgcca tgcacttcac ctcgcccgag gggggcggca cgctcggcac gctcgtggcc
    16561 tggggcaatc atcccgaaac agtctggtcg aaaaacacgg agatcacgtc cgacttctgc
    16621 ggctacttgc gtgaggccct cgaaaaaggc gtcacgatcg acggtcagac gcatgtggca
    16681 ggattgggcg gcacccatgt ctatgtcacc gccgccctcg gcgggctcat gaccacatcc
    16741 ccgtccgtga ccgtccgcga cccgtacctc gaccgggagt tcgagaaacc gtcccacgat
    16801 aaggcgcgcg cccttgggcg ccaactcgcg tcgcgcctct tgcccgtgtt gcgcaccccc
    16861 aacccggccg caaccaacca cgcccccatc ggcgtctgcg cccgcaccat cgaactgcca
    16921 atcgacaacg ccatctttct cctggctccc gtcctgggca tcctcgaccg cggtcatgtc
    16981 cgatggagaa caatgcgcac tgaagtggcc ctggtcacgg ttggcgatgc cagcatcgcg
    17041 tgcctgccgg gcgaagtgta tccggagatc gtcaacggcg gaatcgaacg cgcccccggc
    17101 gccgacttcg aaatcgaccc ggtcgaagtg ccccccatcc gcgccctcat gccgggcaag
    17161 gtcaagttcg tgttcggcct cgccaacgac gaaatcggct acatcatccc caagagccag
    17221 tgggaccgca aaccccctta cctctacgac tcggacacgc ccgtttatgg cgaaatcaac
    17281 tcgctcggcc ccgaaaccgc tccccggttg cacgcggcct tccgcgcgct gtgcggaacg
    17341 tcccagtcgc gctaagggtc tgtgcaaaaa ttcattccga ttttgctgga ggagatttgg
    17401 ctcgccggcg aggcgcgacg aaggagcata gccttgacct ctgtgactgg aggagttgcg
    17461 ggcgtactac cggaccttcc ggccgtcggt gtggctcttt gcccgcagtc gggacccggc
    17521 ccagccgctg ctcgagcgca gtgggcagcg gatgttctgg gacgcggtca ggcgggcggg
    17581 cctgtcgcgt aaaggcggga ttcatagcct gcgtcattgg tttgccaggc acctgttgga
    17641 ggcgggcgtc gaaatcacgg cggtccagcg tctcttgggc cacgccaatc tctcgagcac
    17701 gacgacctac ctgcatgtgc gcgaagaacg gccggcccaa atccagggac cgctccaaca
    17761 actgaaccta ccggccatgc ccgcaatcga gtcggccgcg gcttgaagcc cgacggccac
    17821 cgatgtccac gcctttacgt tccatacaga agcgccgcca tgaatcgttc gcgtcccgac
    17881 aagctttcga tcagcggtgt cgggcacggg tttaatgtcc agtagactat tgacgtcttc
    17941 tcaaaaactg acgacttctt tgtttgttat gattgccgta tgatagtatg tagatcaaat
    18001 agttctcgca acgaaatcat ccatgatcga tacaagcttc aatgtgtata gcgatgcaag
    18061 gggaggtgat cctgatgcga ccagtcccac attgcgtgcc tatcacaagt ttctgtggag
    18121 caagcccctg cctagtggcg accgcttcga cttacgtgac gatgagccag gttattacct
    18181 ttaccacgac tctgcattag gcaacttcgc attggggagt gacgccatca cgcactccta
    18241 taagactcac atcaagaaga agtggcttac gacacaaata ccgaaggaag tgcaggagct
    18301 tttcgatcgc gggtcaacaa ttggcgcata catcgtgttt ccaaataacc aggtggatcg
    18361 gaagcacacg atcaatcagg ccagaggggt atcgcgcctc attgatgatc gattcgattt
    18421 aaccttggag tgcatcagac gcttctatat cggcgagcca aatccgttag gcgagagcct
    18481 caacaggtat gcggcgtttt tccagttgtt tgagacattc aagggctatg tggagttttt
    18541 ccttctcgac gacttggtga atgagcgtgg cgatgtgaag ttctacttgc cattcgatga
    18601 tttccaatcg ccgccagagt tcaaggatgt caaggactac cttgtctaca agaacagagt
    18661 agagtcgttc atcaaatctc gcaacagacg gatcgaagac tatgcgaccc ggacaaagac
    18721 ctaactgacc aaaggaatgt agagaacgga ggaggcgggg caagggcgtg att
//
LOCUS       DAOQRO010000013        18645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2088_length_18645_cov_5.005003, whole genome shotgun sequence.
ACCESSION   DAOQRO010000013 DAOQRO010000000
VERSION     DAOQRO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18645
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_18645_cov_5.005003"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..204
                     /locus_tag="PKK20_01060"
     tRNA            129..204
                     /locus_tag="PKK20_01060"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:162..164,aa:Ala,seq:ggc)
     gene            447..2780
                     /locus_tag="PKK20_01065"
     CDS             447..2780
                     /locus_tag="PKK20_01065"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNU98512.1"
                     /translation="MNTPLVLPMLFLLAAAAIAGESQFHNGGFESGLSGWRLWSRTPG
                     ALTSALENKTVHSDQTALRLEHHGEQDWSLEPALRLEVKPGDLVELDCWVKLAGSGTA
                     TLCASTWDAEGKIVSWSAGERTASEGPNWQHLQARVVASDNVCQMQPRLIGYGPATVW
                     VDDYTVAERGNLHALRSRDLPATLILSNAVLEVRFNTTDAICVVRDRRTSRAWTQKAL
                     ASDCIVKSASIKGAGRLRCELFHGGLGLEVQAVLQLDADRPELVVELSARGPMPASLR
                     FPHPFQGEPGDYLVIPMNEGIAYPDDDATIPPIHLIAYGGHGICMAFFGVTDGDCGQM
                     SIIETPDDASIRVERVDGRLLIVPEWDAQRGEFGYVRRLRHVFFSRGGHVAMAKRYRA
                     YAQQTGQFKTLAEKRQSNSDVDRLIGAVNVWCWDRNAVSLVRELQAAGIERILWSNQQ
                     SPDNVRALNDLGVLTSRYDIYQDVMAPTNFARLQWIHPDWTTNAWPQDILRDRRGNWV
                     KGWAVEAKDGGMIPCGVICDRQALPYARERIPADLVTHPYRCRFIDTTTAAPWHECYD
                     PNHPMTRTESRRWKMELLRYVCDENHLVTGCETGHDAAVPFVHYLEGMLSLGPYRVPD
                     AGRRMSEIWIEVPEAVAKFQLGHDYRLPLWELVYHDCVVAQWYWGDYNNKLPALWDKR
                     DLFNVLYGTPPMFMFNRRLWEENRTRFVQSYRNTCPHVRQVGYQEMTDHRFLTPDRNV
                     QQTVFANGVRITVNFGSADHRLANGRVVPARGFLTGE"
     gene            3085..5010
                     /gene="thrS"
                     /locus_tag="PKK20_01070"
     CDS             3085..5010
                     /gene="thrS"
                     /locus_tag="PKK20_01070"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNU98513.1"
                     /translation="MSNENQPPADRKTLEQRHAMTDLERLRHSCAHVMANAIVKLWPD
                     AQFAAGPPVEQGFYYDVELAHRITPDDFPAIEAEMKAIIKANQPFERVVVTREQALDD
                     ARRGRLAALGDRPVPSKFKIGNLEDIPEGEAISYYKNGDFIDLCAGPHVRRTGNIGAF
                     KLNSVASAYYKGDENNPQLQRVYGIAFKNKSQLDEWLEMQEEAKKRDHRKIGAEMGLY
                     VIDPDYTGPGMPLWLPNGTVLVEELEKLAKETEFAAGYRRVRTPHLAREKMYRTSGHL
                     PYYAESMFPPMELAEQGGGHGSSSGPSDALSEPVSGADDSRPNQPHADRYYLKAMNCP
                     HHHRIFAAAPRSYRDLPLRLAEYGCCYRYEQSGELFGLMRVRSLNMNDAHIYCTEDQF
                     AQEFNAVNDMYLKYFRIFGIEKYVMRFSTHGKEGLGKKYVNEPALWVKTEEMVRQVLI
                     DSKINYIEVPNEAAFYGPKIDVQVWSVIGREFTLATNQVDFAVPAKFGLTYRDRDNTD
                     KTPLCIHRAPLGTHERFIGFLIEHYAGNFPLWLAPEQARVLPVTEAQIDYARGIVDEL
                     RAQQVRAEFEFSNDKLMGRIQRAEAARVHHILVVGGREREANAVAVRIHGKGQQGVRP
                     RSEVIAEMLESIRERRS"
     gene            complement(5077..6570)
                     /locus_tag="PKK20_01075"
     CDS             complement(5077..6570)
                     /locus_tag="PKK20_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98514.1"
                     /translation="MSRSILIIITDFWILSLLVLAKFDAPDVQRPQTNPTISLQGTSA
                     GEKDVVEVLKLALSEEQKRRDALASELSATRQNLDLREQTVAAREQQLRALQQSLREK
                     EQLGVRLAEQRTNLIEQMILAQTNVVFLRDQLQTASTESLLTQEQLKLLQTQLARKEE
                     ELAKTRQRVDELDRQRESTLMEKAQLSAQLQVVETEKRLVTQQVSHLSNEVQIVRAEK
                     STLQQHAIQLAQGVDALAEKSTALVQGVDALAEKSTALTQEIRENRELAANAIFAELA
                     TNRVTLRFAAQRKGLFGIPVQNEKTVQTILVSHANQYSALCHVSDTPLTFSTPGFGFD
                     SFQVSLERGTVARPVAKVMFLSIDPRILIVPLSEAEARQFGVRIYQMAADPLKFTEAV
                     LVGATEGYYGESKFQIDLSAPLYVRMDRSSFRIFSSKFNPSRGDLVLSKTGELLGVMA
                     NNQYCAVLNRLNTIGEIASASETSASAMGSMLSRFDRALQQLPMKLQ"
     gene            complement(6567..8231)
                     /locus_tag="PKK20_01080"
     CDS             complement(6567..8231)
                     /locus_tag="PKK20_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98515.1"
                     /translation="MKSRVVMLTLIALAVLLLVGDALLIPSHVRAVDARVIDLAGRGT
                     PTSVDAGLKFLSLEKTGPAELCLALARRQSVPGYERLEEALQGGGRDHLNGVQWGGVD
                     AVLSHILGNKGDLDNPPQTVVELLVNPVRRETLLDALRRSRRPAVQGILEILTVTNLT
                     LLPPASVPGGQATELVVGVLGLLYQGDYLTGQVRDALAAMVDQSINSGAVRPLEEVLL
                     DTLALVARFDWVQLTSLLSEVTDLATLHELASLCQNAPGQTPTLFVGVVMSGQPAKVV
                     RYVRRWGPPGFVDLEYALGRGKGGVEELLRREQRIHYPRVRSWFESLSPLAQIAPTLT
                     RWAHDWPTVSLGSKYLLLLLAGWLLAWGYRHSSGTYEVPPVPALASLAAGRLWLFALT
                     FVLAMIVLSEPFLARNIQGAEYPLRVQIPVFRSAALAAMVTPIKPYMDQISLVALVSF
                     FVLQLIMYVLNLIKLAEIRRQPITAALKLKYLENEDMMFDAGLYIGLAGSVLSLGCLA
                     LGIIKPSLAAAYSSTLFGIIFVAILKICHVRPVRRRLLLEREQEMV"
     gene            8427..8861
                     /locus_tag="PKK20_01085"
     CDS             8427..8861
                     /locus_tag="PKK20_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98516.1"
                     /translation="MNPSNLHRITNDFQLVRLVSSRKSSVASAIVPRDSGGPFMVAQE
                     GYDPQDVKMTPDTCVLGRSGHWLSTAHFFRMPAADRRREYVFGTAAEVATLLRELPSQ
                     PVILLPDDLPEAAPSSETDDMTTALDAARQSASGPDPGQTTA"
     gene            complement(8881..11280)
                     /locus_tag="PKK20_01090"
     CDS             complement(8881..11280)
                     /locus_tag="PKK20_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HNU98517.1"
                     /translation="MTSRTERLRRQSLETPPSLTAERAVLVTAFYRAHEGRHSVPVMR
                     AKAFYHLCEQKAVWIGEDELIVGERGPGPKAVPTFPELTCHSLDDLRILDSRAKTWYR
                     VPEECLRAYEADVIPYWHGRSMRDRIFAELPADWHEAYGAGLFTEFMEQRAPGHTVLD
                     GKIYAKGMLDFKSEIVRAIERLDFLSDPLALDRLEALKSFDIACDAVMLYARRHAERA
                     RELAGQESDPKRRAELEKIAAVCSHVPAHAPRDFQEAIQYYWFCHLAVITELNGWDSF
                     NPGHLDQHLQPFFERGLADGTLTRASARELLECLFVKFNNHPAPPKVGVTAAESGTYT
                     DFANINIGGLLPDGSDGSNEVSHILLDIVDEMHLLQPSTNIQLSRKSPDALLEHALRV
                     LRKGYGFPSLFNADAVVQEQLRQGKTLEDARAGGCSGCVEVGAFGKEAYILTGYFNMP
                     KVFELALHNGVDSKTGKRLGPETGAPHSFRTFDDLFAAYRAQFRHILEIKLRGNQIIQ
                     RMYATRMPAPFLSVLTDDCIRRGLDYNAGGARYNNTYIQFVGLGSLTDSLSAVNQLCF
                     SPPRAADSPSAPAHGPRLDLGRLVGILDADFAGQEELRQHLLHKMPRYGNDDDRADAI
                     MIRVFNTCVEELDGRPDTKGGRYRIEMLPTTCHVYFGSVTGASPDGRKAGRPLSEGIS
                     PVQGADRNGPTAVIRSAAKMDHIKAGGTLLNMKFAPSLVATEDALRKWGHLVRSYFKM
                     DGHHVQFNVVRAATLREAQERPDEHRDLIVRVAGYSDYFCDLSPELQDEIIERTEHEG
                     F"
     gene            complement(11332..12237)
                     /locus_tag="PKK20_01095"
     CDS             complement(11332..12237)
                     /locus_tag="PKK20_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNU98518.1"
                     /translation="MESGTIFSIQRYSTQDGPGIRTTVFLKGCPLHCAWCHNPESISP
                     RREIVVLETRCVGCGQCREACPFGAAVPSTGPLPPRIDACTLCGACADACPTGARQLI
                     GRTVTVDELALQLARDRVFFEDSGGGVTISGGEPLAQPRFLVALLEACRARGLHTALD
                     TAGFGRWEDLRDAARLTDLVLYDLKAFDSERHRRLTGVSNGLILDNLRLLAGEHRRVW
                     IRVPLVPGFNDDLDDLARMAEFVSRLPHPPLVNLLPFHRTALLKFARLGLDHALDGVA
                     TPSAAALARVREVFANVGLSVKVGG"
     gene            complement(12339..12776)
                     /locus_tag="PKK20_01100"
     CDS             complement(12339..12776)
                     /locus_tag="PKK20_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNU98519.1"
                     /translation="MPAVDVLFDSNVLLYALSDAPGERAKRDQAVFLIATENFGTSYQ
                     VVMETWVVATRKMERRVAPAKLSAFLERILVFPCVPGTDGLYRQAFRLAERLGVHPYD
                     AAILAAAQELGARRVLSEDFNDGQDYDGVRVINPFRGLGGRQR"
     gene            complement(12763..12993)
                     /locus_tag="PKK20_01105"
     CDS             complement(12763..12993)
                     /locus_tag="PKK20_01105"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HNU98520.1"
                     /translation="MKNITLSIDDDLYERSRVLAAQRKSSVSGLVRAYLQSLSDAEQR
                     REQARREIRAMIGHFGGKVGRMPSREERNARG"
     gene            complement(13025..13246)
                     /locus_tag="PKK20_01110"
     CDS             complement(13025..13246)
                     /locus_tag="PKK20_01110"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU98521.1"
                     /translation="MNESDLAKIHMSQRCRVSPEAYLERHYDGRVVEIAPEANRQKGT
                     LQIKVQIQDPDKFLTPELSAKVEFLANSL"
     gene            complement(13275..13499)
                     /locus_tag="PKK20_01115"
     CDS             complement(13275..13499)
                     /locus_tag="PKK20_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU98522.1"
                     /translation="MNRLWGNIKIIVGSICTVVVFTLLLISVSTMSMAVRERFRELAV
                     LKALGFQRREQFGFILAERHVFEFPGQTAG"
     gene            14369..15451
                     /locus_tag="PKK20_01120"
     CDS             14369..15451
                     /locus_tag="PKK20_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98523.1"
                     /translation="MNKALIGAIIIAAGLTSTSLAQTPKQGLLKVALNRQYEYSTNYN
                     PSQMVWKTKKDKITEKTIISALGAVLGHNFTSKAQLTVEEGILGGFQNVDSEDGFPPG
                     ITYPQFTSAAGGARVGVNQVDAGDGPIPGKYQPLGWIFVRDIVAGVYVFVDVTPFFNV
                     SVHECYDCFYLNSFVTDTTFKFGQVTGPPCCGSSSVTSGKGTDKYYMCFEFDNTVANP
                     RVATGDTFGLYSAGGEWLRDLEATMLDPAEDGITPNQAPIYQRFDQSVLRFRVCGIMT
                     YKWTLKESQFIGAATLPSYGYGFVKKVCSMIDGKITVAEYAASTKTKTPAPSDAFETD
                     LEDMWHWQWRYLFYGDEADYFYGGGE"
     gene            15626..16366
                     /locus_tag="PKK20_01125"
     CDS             15626..16366
                     /locus_tag="PKK20_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98524.1"
                     /translation="MRPKVFLTVVVLSIVVLTAAVLLKRPSATVDSEPSVTETTSPNA
                     GSDSPPPAMSGFTGSVQVNPALANVAVPAADKAREEEIARQLDLLREHAASLSEDPAA
                     LAACLDALTHTESEVRQAAVEIVMTSNDTNAIPRLRQALDVVEDPREKVAILDAIEYL
                     ELPDAFDTQLPTNVVYRPGAEALPQEASARLAQPRGENQRTNRFRMRRPYPGAPVQRA
                     ERQGATADRTTTPFAPPGAANPSSPPPQ"
     gene            16782..>18645
                     /locus_tag="PKK20_01130"
     CDS             16782..>18645
                     /locus_tag="PKK20_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNU98525.1"
                     /translation="MTYLPLPSVRLTITNAAGTRQALLYTEAVETSTWSLLTSIDVAS
                     DPVFIDDVATNAARYYTVKRAPVFAASSDSGTNTVDELILFTITNSVSGFEPGSVFYE
                     LAVKPDGAQIDPVTGLITWQTTEKDGPGTNLFKTIVRTGGVYPLTDTNVFAVVVREVN
                     SPPEFLTRPPNQTIDELKRLVYTNAAWDADIPANTLTYRLAEAPNGAQIDPTSGVIQW
                     TPSEAQGPGRYTLTTIVSDHGNPEMTETNSFEVMVQEVNAAPRFRSRPTDRVVEVHAT
                     PVVEVLDDVVVSNAATDSDDIPPNQLTYRLVNPPANSSIDAATGVITWHPKKSQWPGS
                     YVFTTEATDNGVPPLKATNSFTVTMTNKSMVRIPGGTFRMGDMLDNTARSLPVHEVEV
                     GEFYIEQQEVSGALWSEVYQWAIANGYKFDTAATFKGPTYPAANLTWYGAVRWCNARS
                     EMEGRRPAYYTDEARTQVYRGKDKIDIKNEWVDWSSGGYRLPTEAEWERAARIDQGTN
                     GWRFPWGNTISHAEANYTASPLEFVYDVNRDAGPNPVGGKNNPRTSLVSYFRQTPSLY
                     ATTGLALYNMAGNVEEWCWDWSGTYPAERQVDPRGPNQGTYRVRRGGSWTLLADY"
BASE COUNT         3656 a         5758 c         5800 g         3431 t
ORIGIN      
        1 gaaggcgatc cactttccat ccggggaaaa gcgcgggaac atctcgtagc cctggagggg
       61 gatttgggag accgggaatg aattgacaaa gccctcgagc agcctaaggt gcaccctgtt
      121 ttttggctgg ggtcgtagct cagttggtag agcaccacaa tggcattgtg ggggtcaggg
      181 gttcgaatcc cctcggctcc accagccttc cgggcagtgg gaatcgccct cgatttgtcc
      241 gtccccgaga atcaagcagg aaacgactcg tctccggggc attctgcctt cattggaatc
      301 cttgatcggt gaaacatgtt ccagacaagt cgagtttctg tgctgcgttc aacggctcaa
      361 ggattctgga cagacgaacg atcccgctat tcacctcgcg aaatcgcagg ctcgcgcagc
      421 gcgcgactct gtgacatgct cgcgccatga acactccgct ggtccttcca atgctgttct
      481 tgctcgctgc ggcggcgatc gccggtgaga gccaattcca caacggtggt ttcgagtcgg
      541 gtttgagcgg gtggcgactc tggtcgcgga ccccaggcgc cctgacgtct gcgctggaga
      601 acaagaccgt ccactcggac cagacggcgc tgcgtctcga acaccacggc gaacaggatt
      661 ggagcctcga acccgccctc cgtctcgagg tcaagcccgg cgacctggtc gaactagact
      721 gctgggtcaa gcttgcgggt agtggcacgg caacgttgtg cgcgtcgacc tgggacgccg
      781 aaggcaaaat cgtgtcctgg tccgccggcg agcgcacggc gtcggaaggt cccaactggc
      841 agcacttgca ggcccgcgta gtggcttccg acaacgtctg ccagatgcag ccgcgcctga
      901 tcgggtacgg ccccgccacc gtctgggtgg atgattacac cgtcgccgag cgcggcaatc
      961 tccatgcgct ccggtcgagg gaccttccgg cgacgctgat cctcagcaac gcggtgctcg
     1021 aggtccgttt caatacaacc gatgccatct gcgtggtgcg agaccgccgg acctctcgtg
     1081 cctggacaca gaaagcgctg gcgagcgact gtatcgtgaa gagcgcctcg atcaagggcg
     1141 ccgggcggct gcgctgcgag ctgttccacg gcggcctcgg cctggaagtg caggccgtgc
     1201 tgcaactcga cgcagaccgc ccggaactgg tcgtcgagtt gtcagcccga gggccgatgc
     1261 ccgcaagcct gcggtttccg catcctttcc agggcgaacc aggggattac ctcgtgatcc
     1321 cgatgaacga agggatcgct tatcccgacg acgacgcgac gatcccaccg atccatctca
     1381 tcgcctacgg cggccacggc atctgcatgg ccttctttgg cgtgaccgac ggcgactgtg
     1441 gccagatgag catcatcgag actcccgacg acgcgtcgat ccgcgtggag cgtgtcgatg
     1501 gccggctgct gattgtcccg gagtgggatg cgcagcgcgg tgagtttggc tacgtacgga
     1561 gactgcggca tgtgttcttc tcccgaggcg gtcacgtggc aatggcgaag cgataccgcg
     1621 cctatgccca acagacaggc cagttcaaaa ccctcgccga gaagcggcaa tccaattcgg
     1681 acgtggaccg gctgatcggc gcggtgaatg tgtggtgctg ggaccgcaac gctgtctcgc
     1741 tcgtccggga actccaagcg gccggaatcg agcgcattct ctggagtaat cagcagtcgc
     1801 ccgacaacgt ccgcgccctt aacgacctgg gggtgttgac cagccggtat gacatctacc
     1861 aggacgtcat ggcaccgacc aactttgcac gcttgcagtg gattcatccc gattggacca
     1921 ccaacgcctg gccccaggac atcctccgag accgccgcgg caactgggtg aagggctggg
     1981 ccgtggaagc caaggatggc ggaatgatcc cttgcggtgt gatctgcgat cgccaggcgc
     2041 tgccttacgc ccgcgaacga atccccgccg acctcgtcac ccatccttat cggtgccggt
     2101 tcatcgacac caccacggca gctccctggc acgagtgcta cgacccgaac catccgatga
     2161 cccgcaccga aagccggcgg tggaagatgg aattgctccg atacgtctgc gacgagaatc
     2221 atctcgtcac gggttgcgaa acgggtcacg acgccgccgt gccgttcgtg cactacctcg
     2281 agggcatgct gagcctggga ccgtatcgcg tgcccgatgc aggccggcga atgagtgaaa
     2341 tctggatcga ggtgcccgag gcggttgcca agttccagct cggccacgat taccggctgc
     2401 cgctgtggga gttggtctat cacgactgtg tcgtggcgca gtggtactgg ggcgactaca
     2461 acaacaaact gccggccctt tgggacaagc gcgatttgtt caacgtgctc tacggcacgc
     2521 cgccgatgtt catgttcaac cgccgcctct gggaagagaa tcggacgcgg tttgtccaaa
     2581 gctaccgcaa cacctgcccc cacgtccggc aagtggggta tcaggaaatg accgaccacc
     2641 gattcctgac gccggatcgg aacgtccaac aaaccgtgtt cgcgaatggc gtgcggatca
     2701 ccgtcaactt tggcagcgcc gaccaccggt tggcgaacgg ccgggtcgtc cccgccaggg
     2761 gcttcctaac aggcgagtga cggtcaattg cgaaggtcga acgggtgagg gccggcgtga
     2821 gggggctgtg ccgggtatcc agccgactcg gcagtgcagt tcctcggcgc gaccctccgc
     2881 acgaagaatc cgaggtgcgc cccgttgcga cgttgcgatt cggctgtcgg attccatttg
     2941 ccatctggga gggcggcgga taagcttgcg ttcgtactga tgagtttgaa ttctggaacg
     3001 agcacgacga ctttttagcg ttcacgggtc atccgtgaac gcaacgcctc gcactggata
     3061 atggttcgag gcgttttgtt ttttatgtcc aacgaaaacc aacctcccgc tgaccgaaag
     3121 actctcgaac aacggcatgc gatgaccgac ctcgagcggc ttcgtcactc gtgcgcccac
     3181 gtcatggcca acgccatcgt caagctctgg ccggacgcgc aattcgcggc cggcccgccc
     3241 gtcgagcagg gattctatta cgatgtcgag ctcgcccatc gcatcacgcc ggacgatttc
     3301 cccgcgatcg aggcggagat gaaggccatc atcaaggcga atcaaccctt cgagcgtgtg
     3361 gttgtgacgc gcgagcaggc cctggacgac gcccggcgcg gacggctggc cgccctgggc
     3421 gatcgccccg tcccgagcaa gttcaagatc ggcaacctcg aagacatccc cgagggcgaa
     3481 gcgatctcgt actataagaa cggcgatttc attgatcttt gtgcagggcc gcacgtgagg
     3541 cgcaccggca acatcggggc gttcaagctc aacagcgtgg ccagcgctta ttacaagggc
     3601 gacgagaaca atccgcagct ccagcgggtt tacggcattg cgttcaagaa caagagccag
     3661 ctcgacgagt ggctcgagat gcaggaggag gccaagaaac gcgaccatcg gaagatcggc
     3721 gccgagatgg gtctctacgt gatagatccg gattacacgg ggcccggcat gccgctctgg
     3781 ctgccgaacg gcaccgtgct cgtcgaggaa ctcgaaaagc tcgccaaaga gaccgaattc
     3841 gccgcgggtt atcgacgcgt ccgcactccg cacctcgcca gggagaagat gtaccggacg
     3901 tccgggcatt tgccttacta cgccgagagc atgttcccgc cgatggaact ggcggaacaa
     3961 ggggggggcc acgggtcttc gagtgggcct tccgacgcct tgtcggaacc ggtctccggg
     4021 gccgatgatt cgagaccgaa ccagccgcac gccgaccgct actacctgaa ggcaatgaac
     4081 tgtccgcatc accaccgcat ttttgcggcg gcgccgcgca gctaccgcga cttgccgttg
     4141 cgcctggccg agtacggctg ctgctaccgc tatgagcagt cgggcgaact cttcggcctg
     4201 atgcgcgtcc gttcgctgaa catgaacgac gcgcacattt actgcaccga ggatcagttc
     4261 gcccaggagt tcaacgccgt gaacgacatg tacctgaagt atttcagaat cttcggcatc
     4321 gagaaatacg tcatgcgctt cagcacccac ggcaaggaag ggctcggcaa gaagtacgtc
     4381 aacgaaccgg cgctttgggt gaagaccgag gagatggttc gccaggtcct gatcgactcg
     4441 aagatcaact acatcgaggt gcccaacgaa gcggcgttct atggccccaa gatcgacgtc
     4501 caggtctgga gcgtcatcgg gcgcgagttc acgctggcga ccaaccaggt cgatttcgcc
     4561 gtgccggcca agttcggcct gacctatcgc gaccgggaca acacggacaa aaccccgctc
     4621 tgcatccatc gcgcgccgct cgggacgcat gagcggttca tcggattcct gatcgagcat
     4681 tacgcgggca atttccccct ctggctcgct ccggagcagg cgcgggttct gccggtgacc
     4741 gaggcgcaaa tcgactacgc cagggggatt gtggacgaac tgcgcgcgca gcaggttcgc
     4801 gcggagttcg agttcagcaa cgacaagctg atgggccgca tccagcgcgc cgaggcggcg
     4861 cgcgtccacc acatcctggt ggtcggcgga cgcgagcgcg aggcgaacgc cgtcgcggtg
     4921 cgcatccacg ggaaaggcca acagggcgtc aggccgcgct cggaggtgat cgccgagatg
     4981 ctcgagtcca tccgcgagcg gcgatcctaa cacgcatcgc ggtcctcttt cgaagactga
     5041 ccgccctgtg cgctcgcggg gtgggaaata tgcgggctac tgcaatttca tcggcagttg
     5101 ctgcaaggcg cggtcgaagc gggagagcat cgagcccatg gccgaggcgg atgtctcgga
     5161 ggcggaagcg atctcgccga tcgtgttcag gcggttgagc accgcgcagt actggttgtt
     5221 ggccatcacg cccagcaact cgcccgtctt gctcagcacg aggtcgccgc gcgagggatt
     5281 gaacttgctc gagaagatcc ggaacgaact gcgatccatg cggacataga gcggggccga
     5341 aaggtcgatc tggaacttgg actccccgta atagccctcc gtagcgccga cgagaaccgc
     5401 ttcggtgaac ttgagcggat cggccgccat ttgatagatt cggacgccga actgacgcgc
     5461 ctccgcctcg ctcagaggga cgatgagaat gcgcggatcg atggacagga acatcacctt
     5521 ggccacgggg cgcgccacgg tgccgcgttc gagactcact tgaaacgagt cgaatccgaa
     5581 tcccggcgtc gagaacgtga ggggggtatc cgacacatga cagagggccg agtattggtt
     5641 ggcgtgggaa accaggattg tctggaccgt cttttcgttt tggacgggga tgccgaaaag
     5701 gcccttgcgc tgggcggcga accggagcgt gacccgattg gtggccagct cggcgaagat
     5761 ggcgttggca gcgagttcgc ggttctctcg aatctcttgc gtgagggcgg tcgatttctc
     5821 cgccagagca tccacgccct ggaccagcgc ggtggatttc tcggcgaggg catccacgcc
     5881 ctgggcgagt tggatggcgt gctgctggag ggtgctcttc tcggcgcgca cgatctggac
     5941 ctcgttgctg aggtggctga cctgctgggt gaccaggcgt ttctcggtct cgaccacctg
     6001 caactgcgct gacaactggg ctttctccat gagggtggat tcgcgctggc gatccagttc
     6061 atcgacgcgc tggcgggtct tggccaattc ctcctccttg cgggccagtt gggtctggag
     6121 cagcttcaac tgctcctggg tcagcaggct ctcggtgctg gccgtctgca actggtcgcg
     6181 caggaagacc acattggtct gggccaggat catctgctcg atcaggttgg tgcgctgctc
     6241 ggcgagtcgc acgccaagct gttctttctc gcgcagggac tgctgcagag cccgcaactg
     6301 ctgctcgcgc gccgccaccg tctgctcccg cagatcgagg ttctggcgcg tcgccgacaa
     6361 ttcggacgca agggcgtcgc gccgtttctg ctcctcgctg agcgcgagtt tgaggacctc
     6421 gacgacgtcc ttctcccctg cgctggtgcc ctggagggag atggtcgggt tggtctgcgg
     6481 ccgctggaca tcgggcgcat cgaacttggc gaggaccagg aggctgagaa tccagaagtc
     6541 cgtgatgatg atgaggattg agcggctcat accatttcct gctcccgttc gagcagcagc
     6601 cgccgccgca cgggacgcac atgacagatt ttcaggatcg ccacgaagat gatgccgaag
     6661 agcgtcgagg agtaggcggc agccaggctc ggtttgatga tgcccagggc gaggcagccc
     6721 agggacaaga cggacccagc caacccaatg tagaggccgg cgtcgaacat catgtcctcg
     6781 ttctcgaggt acttgagttt cagcgccgcg gtgatcggct ggcggcgaat ctccgccagc
     6841 ttgatgaggt tcagcacata catgatgagc tgcaggacaa agaaggacac caaggccacg
     6901 agactgattt ggtccatata cggtttgatt ggagttacca ttgcggccag cgcggcgctc
     6961 cggaacaccg ggatctgcac gcgaagcgga tactccgccc cctgaatgtt ccgggcgaga
     7021 aacggctcgc tcagcacaat catcgccagc acaaatgtca gcgcaaacag ccagagccgc
     7081 ccggcagcca ggctggccaa ggccggcacg ggcggcacct cgtaagttcc cgacgagtgc
     7141 cgatagcccc aagccaacaa ccagcctgca agcagcagca gcaaatactt cgatcccaag
     7201 gaaaccgtcg gccagtcatg cgcccaacgc gtgagggtcg gcgcaatctg cgcgagcgga
     7261 gacaggctct cgaaccagga ccgcacccgc ggatagtgaa tccgctgctc gcgccgcagc
     7321 aattcctcga ccccgccctt gccccgcccg agagcatatt cgaggtcgac aaaaccgggc
     7381 gggccccaac gccgcacata gcgcacgacc ttcgccggct gtcccgacat caccacgcca
     7441 acgaacaaag tgggcgtctg gcccggggcg ttttggcaga ggcttgccag ttcatggagt
     7501 gtggccagat cggttacctc gctcaacaga ctcgtcaact gcacccaatc gaagcgcgcc
     7561 accaaagcca gcgtatcgag cagcacttcc tcgagagggc gaaccgcgcc gctgttgatc
     7621 gattgatcga ccatcgcggc cagcgcgtcc cggacctggc cggtcaggta atcgccctgg
     7681 tagagcagac cgagcacccc gaccaccaac tcggtggcct ggccgcccgg gaccgaagcc
     7741 ggcggcagca gggtgaggtt ggtgacggtc aggatttcca gaatcccttg aaccgcggga
     7801 cgacgcgacc ggcgcaacgc atcgaggagc gtttcccgcc gcacgggatt gacgagcaac
     7861 tcgaccaccg tctgcggcgg gttatccaaa tcgcccttgt tgcccaggat atggctcaac
     7921 accgcatcca ccccacccca ctggacgccg ttgaggtgat ctctccctcc gccctgcaac
     7981 gcctcctcaa gccgttcata tccagggacg gactgccgcc gcgcaagcgc cagacaaagc
     8041 tccgccggcc ccgtcttctc gagggacagg aacttcaggc cggcatccac cgaagtcggc
     8101 gtgccgcgtc cggccaagtc gatcacccgc gcatcgaccg cgcggacgtg ggaggggatc
     8161 aacaacgcgt cacccaccag cagcagcact gccagggcga tcagcgtcag catgaccacg
     8221 cgtgatttca tccaaaacaa acttctcgcc acgcatcggc caggtttcga gcgatggttc
     8281 ttagcattca gacgcagcga ccctctgtgt attcgtgagt ttggcctctt ttccaagcac
     8341 aaacttacgc tttgcgccgg atgccgcgat ccgccatggt ccgatcccga cgtccatgca
     8401 tcgatcgcca taggcccagc gcagccatga acccctcaaa cctccatcgg atcacaaacg
     8461 actttcagct cgtccgcctg gtcagttcgc gcaaatcgag cgtcgcctcg gccatcgtcc
     8521 cgcgcgattc gggcggccct ttcatggtgg cccaagaggg ttacgatccc caggacgtca
     8581 aaatgacgcc tgacacgtgt gtccttggac ggtcagggca ctggctttcc accgcgcact
     8641 tcttccggat gccggcggcg gaccgccgcc gcgagtatgt gttcggcacc gcagcggagg
     8701 tcgcaacatt gctccgggag cttccctcgc aaccagtgat tctcctccct gacgatctgc
     8761 ccgaggcagc gccctcttct gaaaccgacg acatgacgac ggccctcgac gccgcccgcc
     8821 aaagcgcctc cgggcccgat ccaggacaga cgacagcgtg aggccagatt ccggtcaagg
     8881 ctagaatccc tcgtgttcgg ttctctcgat gatctcgtcc tgcagctcgg gcgagagatc
     8941 gcagaaatag tcgctgtacc ctgccacgcg gacgatgaga tcgcggtgct cgtccgggcg
     9001 ttcctgggcc tcgcggagcg tggcggcgcg gacgacattg aactggacat gatgtccgtc
     9061 catcttgaag taactccgca ccagatgccc ccatttgcgc agggcatcct cggtggccac
     9121 caacgacggc gcgaatttca tgttgagaag cgtgccgccc gccttgatat gatccatctt
     9181 ggcggcgctt ctgatcaccg ccgtggggcc gttgcggtcc gcgccctgga ccggcgagat
     9241 gccctcgctc aggggacgcc cggcctttcg tccgtccggg gaggcgccgg tcacgctgcc
     9301 gaagtagacg tggcaggtcg tgggcaacat ctcgatccgg tagcggcccc ctttggtgtc
     9361 ggggcgccca tcgagttcct cgacgcacgt gttaaagacc cggatcatga tggcatccgc
     9421 gcggtcgtcg tcgtttccgt acctgggcat cttatgcagc agatgctgcc gcaactcttc
     9481 ctggccggcg aaatcggcat cgagaatgcc cacaagccgg cccaggtcga gccggggacc
     9541 gtgtgccggg gccgagggag aatccgccgc tcgcgggggg gagaaacaga gttggttgac
     9601 ggcgctgagg ctgtcggtga ggctgcccag gccgacgaac tggatgtagg tattgttgta
     9661 gcgcgccccg ccggcgttgt agtcgagccc gcgccggatg cagtcatcgg tcagcacgct
     9721 gaggaacggg gctggcatgc gggtggcgta catgcgctga atgatctggt ttccgcgcag
     9781 tttgatctcg aggatgtggc ggaactgggc ccggtacgcc gcgaacaggt cgtcgaatgt
     9841 tcgaaaggaa tgcggggcgc cggtttcggg gccaagccgc ttcccggtct tggaatccac
     9901 cccgttgtgc agcgccagct cgaagacctt cggcatgttg aaatatccgg tcaggatgta
     9961 ggcttccttg ccgaacgccc ccacctcgac gcatccgctg cacccgcccg cccgcgcatc
    10021 ttcgagcgtc ttgccctgac gcagttgctc ctggaccacg gcgtcggcgt tgaagaggct
    10081 cgggaatcca tatcccttgc gcagaacgcg tagagcgtgt tcgagcaagg cgtcggggct
    10141 cttgcgagag agctggatgt tggtgctcgg ctgaagcagg tgcatctcgt cgacaatatc
    10201 gaggaggatg tgggacacct cgttcgagcc atcggaacca tcgggcagga gtccgccgat
    10261 gttgatattg gcgaagtcgg tgtacgtgcc gctctctgcc gccgtcacgc ccaccttggg
    10321 cggggcggga tggttgttga acttgacgaa cagacactcg agcagttcgc gcgcgctcgc
    10381 gcgcgtcagc gtcccgtccg caaggccgcg ctcgaaaaag ggctgcaggt gctggtccag
    10441 gtgtccgggg ttgaacgagt cccagccgtt gagttcagtg atcacggcca gatggcagaa
    10501 ccagtaatac tgaatggcct cctggaaatc ccggggggca tgcgccggca cgtgcgaaca
    10561 gacagccgcg atcttctcca gctcggcccg ccgcttcgga tcgctctcct gtcccgccag
    10621 ttcccgggcc cgctccgcgt ggcgccgcgc atagagcatc acggcgtcgc acgcgatgtc
    10681 gaacgacttg agcgcttcga gtcggtcgag cgcaagcgga tcggacagaa agtcgagccg
    10741 ctcgatcgcc cggacgatct cgctcttgaa atccagcatg cccttggcgt agatcttgcc
    10801 atcgagcacc gtgtgcccgg gcgcgcgctg ctccatgaac tcggtgaaca gcccggcgcc
    10861 gtatgcctcg tgccaatccg ccggcagctc cgcgaaaatt ctgtcgcgca tcgaccgccc
    10921 gtgccaatag ggaatcacat ccgcctcgta ggcccgcaga cattcctccg gcacgcggta
    10981 ccaggtcttg gccctcgagt cgagaatccg caggtcatcg agactgtggc aggtcagttc
    11041 gggaaaggtc ggcaccgcct ttgggccagg cccgcgctcg ccgacaatca gttcgtcctc
    11101 gccaatccag acggcctttt gctcgcagag gtgataaaag gccttggccc gcatcacggg
    11161 cacggagtgc cggccctcat gggcccggta aaacgcggtg accaacacgg cccgctcggc
    11221 agtcagactg ggcggcgtct cgaggctttg ccggcgcaga cgttccgttc tcgatgtcat
    11281 gcccccacct tagcggcctc ttggcgtggg cgaaagccaa gaatcggccc cctatccccc
    11341 gaccttcacc gacagtccga cattggcaaa cacctcgcgc acgcgcgcca gcgcagcggc
    11401 cgacggggtc gcgacgccgt ccaaagcgtg atcgaggccc agacgcgcga atttgagcaa
    11461 cgcggtgcgg tgaaacggca gcaggttcac caggggtggg tgaggcagac gcgacacgaa
    11521 ttcagccatg cgcgcgaggt catcgagatc gtcgttgaag ccggggacca gcggcacgcg
    11581 aatccagacg cggcgatgct ctcccgccaa caacctgaga ttatcgagaa tcaggccgtt
    11641 ggacacgcct gtcagacgcc gatgccgctc gctgtcgaag gccttcagat cgtagagcac
    11701 aagatcggtc aggcgcgccg cgtcccgcaa atcctcccag cgtccgaacc ccgcggtgtc
    11761 gagcgctgtg tgcagcccac gcgcgcggca agcctcgagc agggcgacga gaaaacgcgg
    11821 ctgcgccagg ggttccccgc ccgagatcgt cacgccccca cccgagtcct caaagaagac
    11881 tcggtcccga gccagttgca gcgccagctc atcaacggtc accgttcgcc cgatcaactg
    11941 gcgcgcgccg gtgggacaag cgtccgcgca cgcgccgcag agggtgcagg cgtcgatgcg
    12001 cggcggcagc ggcccggtgc tcgggactgc ggctccgaac ggacaagcct ctcgacattg
    12061 tccgcagccc acgcagcgcg tttccagcac gacgatctct ctgcggggcg agatgctctc
    12121 cgggttgtgg caccaggcgc agtggagggg acagcccttg agaaaaacgg tcgtgcggat
    12181 gcccgggccg tcctgggtcg agtatcgctg gatgctgaat atggtcccgc tttccacgcg
    12241 gccgcaagtc tacgccagcc tgctccccgt ttcacgcacg gagtgcgacc gttgcgaatc
    12301 cccgaatctc aactctcacc ccgtcggttc atggagactc accgttgcct gcccccgagt
    12361 cctctgaagg gattgatgac tctcaccccg tcgtagtcct gaccgtcgtt gaaatcttcc
    12421 gatagcacgc gccgggcgcc gagttcctgt gcggcggcca gaatggctgc gtcatagggg
    12481 tgaacgccca atcgctcggc caaccggaaa gcctggcggt aaaggccgtc cgtgccgggg
    12541 acgcacggga acaccagaat cctctcgaga aaggccgaga gcttggccgg ggcaacccgt
    12601 cgctccattt tgcgtgttgc gacaacccac gtttccatca ccacttggta ggaagtccca
    12661 aagttctcgg tggcaatcaa aaacaccgcc tgatcccgtt tggcgcgctc gcccggcgca
    12721 tcggagaggg cgtacagcag aacattgctg tcgaacagga cgtcaaccgc gggcattgcg
    12781 ctcctcccgt gaaggcatcc gaccgacctt gcccccgaaa tgcccgatca tggcccgaat
    12841 ctcgcgccgg gcctgctccc gccgctgctc ggcgtcggag agactctgca gatacgcgcg
    12901 caccaggccg gacaccgacg acttccgttg ggccgccaga actcgcgatc gctcatagag
    12961 atcgtcatcg atgctgaggg tgatgttctt catgccgaca cattaactgt gcaactgtgg
    13021 accgtcaaag tgaattggct aagaactcga ccttcgcgct cagttccggc gtgaggaact
    13081 tgtccgggtc ttgaatctgc accttgatct gcagggtgcc cttctggcga ttggcttccg
    13141 gtgcgatttc aacgacgcgg ccgtcgtagt ggcgttcgag gtaggcttcc ggcgaaacgc
    13201 ggcagcgctg gctcatgtgg attttggcga gatcggattc gttcatgcgc cggatgaggt
    13261 tcgtcggtgc ggcttcatcc cgcggtctga ccgggaaact cgaatacatg tctctcggcg
    13321 aggatgaacc cgaactgttc ccgccgctgg aagcccagcg ccttgagcac cgccagttcg
    13381 cggaaccgtt cccgcaccgc catactcatg gtgctcaccg agatcagcag tagggtgaac
    13441 acgaccacag tgcagatcga gccgacgatg attttgatgt tgccccacag tcggttcatc
    13501 ggccgcgacg aggctcgggt cgatcacaag cgcccgcgcg atcgcgatcc gctgttcctg
    13561 gctgccgcag aaactccctc gctgcaatgt ccagcgacac ccggtcgagg gctgtcgcct
    13621 gaatcgcgct ctgctcatac accttcgaga gctcgcggat gcgcacgatc ggttcgcccg
    13681 ccataaaatc gtgcgacgaa tgcgccaaaa agggacgaaa cgcaaactca gttcccacgc
    13741 gtgcagcagt ccgattctca ttgcagtacg ccccgtccgt gcacagggcg gtgagacatc
    13801 acaagaggac tgccatgcgg actggaaaag caacaccgcg cccgaccacc gccgatctcg
    13861 acacgcgccg cgtcccgatc cacgggacgg gcaataggga atgcgcacgg ttcttgctcc
    13921 tcctgtcctt tccgcggaac gccgtttcgc gaaggtcctc gtccccggcc gcgcgaggac
    13981 gaatcatgcc tgggcgctcg ggcgcaaccg actgttgaaa ttggtgtctc acgatgccaa
    14041 atcctggccg ggaacacagg gccggacgca gcctttctga ggccgaaaag aagggaagcg
    14101 gacgggagaa gcgcagcgcg ttttttttgg cgcaatcatg tttgacctcg ccgcaacatg
    14161 atgtataatc ttgggctgga attggctgca aggcgtcggg cctacgaaat gctgcgaaat
    14221 gctgcgaaat gctgcggtgc attttcttgg aaaagtcgtt gacaccccct cgaggttgtg
    14281 acgtaatctg cccggaaaca gtgggcgtga agatggcccg cgtgcggatc agctggaaca
    14341 taactggtaa caacatcaag tcgaaagtat gaacaaagca ctgatcggag caataatcat
    14401 tgcggctggt ttgacgagca cgtcgctcgc tcaaaccccc aagcaagggc tgctgaaggt
    14461 ggccttgaat cggcagtacg agtattccac caactacaac ccgagccaga tggtttggaa
    14521 gacgaaaaag gataagatca ccgaaaagac cattattagc gctctcggcg cggttctcgg
    14581 gcacaacttc acgagcaagg cgcagttgac tgtggaggag gggatactgg gcggttttca
    14641 aaacgttgac agtgaggatg gttttcctcc cgggatcacc tacccgcagt tcacgtctgc
    14701 ggcgggcggg gctagggtcg gtgtgaatca ggtcgacgcg ggcgatggtc cgattcccgg
    14761 caagtatcaa ccgctgggct ggatcttcgt gcgcgacatt gtcgcgggcg tttatgtgtt
    14821 cgtggatgtg acgccgtttt tcaatgtgag cgttcacgaa tgctacgact gcttctacct
    14881 gaacagcttc gtcacggaca cgaccttcaa gttcggtcaa gtcaccggtc cgccttgctg
    14941 cggttcgtct tcagttacgt ccggcaaagg caccgacaaa tactatatgt gcttcgagtt
    15001 cgacaacaca gtggcgaacc cgcgtgtcgc gaccggcgac acattcggcc tctactccgc
    15061 tggtggcgaa tggttgcgcg acctcgaggc gactatgctc gatccggcag aagacgggat
    15121 caccccgaac caagcgccga tctatcagag gtttgatcag tcggtgctcc ggttccgtgt
    15181 ttgcggcatc atgacgtaca agtggacgtt gaaggagagc cagttcattg gcgccgcaac
    15241 gctcccctcc tacggctacg gtttcgtcaa gaaggtctgc tcgatgatcg atggcaagat
    15301 cactgtggcg gagtacgccg ccagcaccaa gaccaagacc cccgctcctt cggatgcgtt
    15361 tgagaccgat ctggaggata tgtggcattg gcagtggcgc tatctgttct acggtgatga
    15421 ggccgactac ttctacggtg gtggtgaata gtagtcacag cgagtccaag tcatttgcaa
    15481 ttttttaaag actaaagcag ttgtcacgca atccaaggga ggctggagtg agccaccttg
    15541 gattgcgctt ttttgagacc gggtttcctc gcaagttcag aggcgcctcg tgtggcgcga
    15601 tagttgctta cggaggctca tcattatgcg accgaaggtg ttcttgaccg ttgttgttct
    15661 gtcgatcgtt gtgctgaccg ctgcggttct tctcaagcgt cccagcgcga ctgtcgactc
    15721 tgaaccgtcc gttacggaga cgaccagtcc gaacgccgga tcggacagtc cgcctccagc
    15781 catgagcggc ttcacggggt ctgtccaggt gaatcccgca ctcgccaatg tggcggtgcc
    15841 cgcagccgac aaggcacgcg aggaggaaat cgccagacag ttggatttgc tccgcgaaca
    15901 tgcggcgagt ctttctgaag atccagcggc gttggcggca tgcctcgacg cactcaccca
    15961 caccgagagc gaagtccgtc aggcagcggt cgagattgtc atgacgtcga atgacaccaa
    16021 cgcaatcccg cggctgcgcc aggctctcga cgtcgtcgag gaccctcgag agaaggtcgc
    16081 gattctcgat gcgatcgagt acctcgagct ccctgatgcg ttcgacaccc aattgccgac
    16141 caatgttgtg tatcgtcctg gagccgaggc tttgccccag gaggcgagcg cgcggcttgc
    16201 gcagcctcgc ggagagaacc agaggacgaa tcgctttcgc atgcgcagac cgtacccggg
    16261 tgctcccgtc cagcgtgctg aacgtcaagg agcgacggcc gaccgcacca caacaccgtt
    16321 tgctcctccg ggcgccgcca acccgtcatc tccacctcca cagtaacgcg gtcggctcag
    16381 agcacgggag gagttggcgc gattcccacc gcagatgacc ggcactattt tctgccggct
    16441 gctgatgccg cccccaaaac tggatttcta tgcttctggt gctccaggac cccggtccag
    16501 agagcggatg cgggtccggg cacgccgggc gattggggaa tcggggggag agggtccgga
    16561 catgtgtttg gggtgatggt gaattcttgt gcataaatac tgaagtacgt ttgtcgacaa
    16621 cgacggaaga aagtgagttc tgcatggctg tgattggttc tacgatgaga gaattggcga
    16681 ccaggaaact cggaaccctg gtgtgcggca tgttgatggc tgtgactgca ggtccagtgt
    16741 ggagcgccga accagggccg gcatctgggc tccagattca attgacatac ctcccgcttc
    16801 cgtccgtgcg gttgaccatc accaatgctg ctgggacgcg ccaggcactc ctctacacag
    16861 aagcggtcga gacgagcacc tggagcttgc tgacgagcat tgatgtcgca agcgatcccg
    16921 tgtttatcga tgatgtcgcg acgaatgccg cgcgatacta caccgtgaaa cgggctcccg
    16981 tgttcgcggc cagctccgac tcgggcacga acacggtcga tgagttgatc ctcttcacga
    17041 tcaccaacag cgtttctggc ttcgagcccg gctcggtgtt ctacgagctt gccgttaagc
    17101 cggacggcgc gcagatcgat ccggtgacgg gtttgatcac atggcaaacg acagagaagg
    17161 atgggcccgg aaccaatctc tttaagacga tcgttcggac cggtggcgtc tatcctttga
    17221 cggacaccaa cgtctttgca gttgtggtga gagaagtgaa cagcccgccg gagtttctga
    17281 cacggccgcc caaccagacc atcgatgaac tgaagcggct cgtgtacaca aatgcggctt
    17341 gggacgcgga tatccccgca aacacgctga cctaccggct tgccgaggct ccgaacggcg
    17401 cgcagatcga cccgacgagc ggagtgatcc agtggacgcc atccgaagcg caaggtcctg
    17461 gtcgctacac gcttacgact atcgtcagcg atcatggcaa tccggagatg actgaaacca
    17521 attccttcga ggtcatggtt caggaagtga atgcggcgcc caggttccgc agccgaccga
    17581 cagaccgcgt ggtcgaggtg cacgcgaccc cggtggttga ggttctggac gacgtggtcg
    17641 tcagcaatgc tgcgactgat tcggacgaca ttccgccaaa ccagctcact taccgccttg
    17701 tcaacccgcc tgccaacagc tcgatcgatg cggcgaccgg cgtcatcacc tggcacccga
    17761 agaagagcca gtggccgggc agctatgttt ttacgacgga agcgactgac aatggagttc
    17821 cgccgctcaa ggcgacgaac agcttcaccg tgacgatgac aaacaagagc atggtacgga
    17881 ttccgggcgg gaccttccgg atgggtgaca tgctggacaa caccgcgcgg tcgcttccag
    17941 tgcatgaggt tgaggtgggc gagttttaca ttgaacaaca ggaagtctcg ggcgcgctct
    18001 ggagtgaggt ttaccaatgg gccattgcga atggctacaa gttcgatacg gcggcgacgt
    18061 tcaaaggccc cacctatccg gctgcgaatc tcacctggta tggcgctgtc agatggtgca
    18121 atgcgcgctc cgagatggaa ggacggcgac cggcctacta tacggacgag gcgaggacgc
    18181 aagtgtacag gggaaaagac aagatcgaca ttaagaacga gtgggttgac tggtcctcgg
    18241 gagggtatcg gctgcccacg gaggcggaat gggagagggc ggcgcgcatc gatcaaggca
    18301 caaacgggtg gcgattcccg tgggggaata ccatctcgca cgccgaggcg aattatacgg
    18361 cttcaccttt ggagttcgtc tacgatgtca accgtgatgc cggcccgaat ccggtcggcg
    18421 gcaagaacaa cccgcgtacg agtctcgtga gctactttcg ccaaacaccg tccctctatg
    18481 caacgacagg cctggccctg tacaatatgg cggggaatgt cgaggaatgg tgctgggatt
    18541 ggtctggcac ctatcccgcc gaacggcaag tcgatcctcg cggtccgaac caaggcactt
    18601 accgtgtaag gcggggcggt tcttggacct tgctcgccga ttacg
//
LOCUS       DAOQRO010000014        18615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2092_length_18615_cov_5.426940, whole genome shotgun sequence.
ACCESSION   DAOQRO010000014 DAOQRO010000000
VERSION     DAOQRO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18615
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_18615_cov_5.426940"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..950
                     /locus_tag="PKK20_01135"
     CDS             141..950
                     /locus_tag="PKK20_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HNU98526.1"
                     /translation="MSTAYHVVVCASLVPDPLQTLEPVAGPAGPALKNEAMLPFVLDP
                     WAAHALYEAAHLAKNRPGTKVWLVSLGPKPKLQQVMMTVAQKVPFEFVPLDGSASGFA
                     EASAVAAALAEGIQAIPGLDRSKLLVFGGWESASRGAGVTLSMVGERLGIIDQFQGVD
                     ELKIQDDGAFEVVERLEGGQHQISRCAGPPALFGWATGHLPEPKNNPQIGMINMRTLM
                     PALQKAKPIQLADAGLSFDSVVLPKQLRETRIVKDASAEDVAREIVDWISK"
     gene            959..1999
                     /locus_tag="PKK20_01140"
     CDS             959..1999
                     /locus_tag="PKK20_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HNU98527.1"
                     /translation="METILFLAATEPDGSLAPAALEALGAARSLAAALAGCSLTVGLQ
                     GPSVQTAADRIAGCGATRFLGVAGPDFGSARYASDALAAEALVRASQATVVVAPGTSR
                     LSRCLPGVAQRTGGQADTHVTALGARESRPFVNRWYYRQRMEAVVQRTQRPWIVLIDP
                     GSQSPWDGPPGGAVAVEMIDVTLPEAARKTSVVGVRAPAADAQTIRPDADLLFVAGAG
                     WTKKQADGQARVVEAEQIILEFLKRSRASLGSTKSLVDLGGEGQTVLSFLSHLNQIGQ
                     TGATPRHPKGLSTCCHGEEPHTVGWRFINERRAISLDPNCGWARGKADVLYVADAFAV
                     MREVNRLLAANA"
     gene            2024..2917
                     /locus_tag="PKK20_01145"
     CDS             2024..2917
                     /locus_tag="PKK20_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNU98528.1"
                     /translation="MRLCVVFNPTARGNNAKRFRRRLEARARDCVLMPTTGPGAAVDL
                     AAEAVRQGFDTVVAAGGDGTVNEVLNGIAGVEHGLERTRLGILPLGTMNVFARELGLP
                     LNPDAAWCLLRESNETAIDLPWAEFEVEGRTTRRCFAQLAGAGLDSRAIGLVDWTWKT
                     RIGSLAYGIAGLRAMRGPQPLVTVTLPDGTTSSGELVLVGNGTLYGGCVTMFPGASLR
                     DGQLAIRVLPRIGVVTMARFALSWLFRGGFAVPGERHLRADRFTLTSGEAVPFQVDGD
                     NAGPLPARFSVRPRALRVVAP"
     gene            complement(3169..4773)
                     /locus_tag="PKK20_01150"
     CDS             complement(3169..4773)
                     /locus_tag="PKK20_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNU98529.1"
                     /translation="MSQRVFSPAVFALVCGCLAAPRAPAGDPEAFARNPIIHADVPDM
                     AMIRVGDTYYMSSTTMHMSPGLPIMKSKDLVNWTLVSYAYDTLEDVDALNLNNGKSAY
                     GGGSWASSIRHHDGTYVVTTFSQTTGKTHVYTTPDIEKGPWKATSFRPMLHDHSLFFD
                     DDGRVYMLYGGGDLRLVELNPDFSGLKQGGFNQVVVTNASAVAGAAIGLPAEGSQLLK
                     VNNTYYLFNIVWPRGGMRTVIVHRANTIAGPYVGRVALQDKGVAQGSLIDTPGGDWFA
                     YLFRDFGAVGRIPYLVPVQWKDGWPVFGIEGKAPDRLDLPPSRGLIPGIVASDEFDRA
                     AGEPALPLVWQWNHNPDNTLWSVSQRPGFLRLTTGRTDAHLLAARNTLTQRTIGPACS
                     GTTSVDARNIKDGDYAGLALLQHRYGFVGLTSEAGATFVVMVNAQSNAPIEIERVAVP
                     SQTVFLKTECDFRNRTDTARFCYSLDGTSWTAIGNELKMSYTIPHFMGYRFGLFCYAT
                     KAPGGFADFDFFRISDKRGGPDRAPR"
     gene            complement(4803..8180)
                     /locus_tag="PKK20_01155"
     CDS             complement(4803..8180)
                     /locus_tag="PKK20_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNU98530.1"
                     /translation="MKSTRQPMRRLVLGALLAGCGLSVTSRLAAQTIPLDGLVARWDF
                     EEGSGAIAHDTSGPNANHGTLDNYTDDAQWVAGRFGGGLAFTGATSNRLVVPSHASIA
                     ADLANGFTVSAWFRSNVPLASNASTYALLEKGNMFFLLQGVATEGGGMNFLLKKGTVN
                     QTVGIVDALSADTWYHVAGVFTGTEALVYLDGALKGRRAVAAPIDVTDLALVIGGDDL
                     NRVFNGTIDQVLIWNRPLTAEEILQVAARVGAPTVTGQPQAQSVYAGGTATFSVSAAG
                     QNPLRYLWYRNDQPLRTQTAETLVVEYATPADAGLYRVEVSNDIGTAKSQSASLEVRS
                     VEGLQTARVLSMHFNETSGLTASDASGNGNNGQLSGYWDETSHWSAGRVNGSLSFDGV
                     ASLVSVPQSQSLSQVQGEATFAFWIHPLGWGVPEDVGVYTRSASYILRKADQFGIRLI
                     NDPGTVVQTLVSRAGRGADAGGVVRSAFEVNAPQGSVALDQWQHWVVLYRNNTVSFFR
                     NGFRVGDPVPGTLGPPAEAGLVIGAYDDLATVTSASRLEGRLDELGIWSRPLSEAEIL
                     ELAGQDVVGVPVIVQQPAGQKRIEGTTAMFEVFVTGKRPLEYQWLKNGIPIAGAVQNT
                     LTLNRLQPADAGGYAVRVTNSEGMTTSEAALLEVEALDAITSGLVAYYTFDDGPGTTL
                     TDSSGNNFHGQLVNMDDASRIAGPIGGAFRFDGSDDYIVVGHDPMLDLTTEATVSVWL
                     KVDGMSPANHDRVIRKGTNFDFVLLPGGVVRTYGIGKAPYDGPAQSWELGIWTHFAYV
                     YRGGTVQWYRNGEPLGALLSGRLGELTADPLVIGNYAAGLTINRPYVGSIDDLGIWQR
                     ALSPSDILGIYVNGQQGKPLNQEFRPLTITAIKPAAEAVALEFYTPFNNRPAQIESKA
                     ALSDPAWLVVPNLTFVDLGQGLFSASIPVGTAPAGFFRAVALPPPPLFFDDFEQAVPG
                     WTHAGNEDRWERGAPTVGPTAAYSGANVYGTGLNRNVGAYSYCWLQSPEIDLTGVSSA
                     TLRFAEWLNLDFLAGVPVEQQAHQVMINVLDASTLATVSDAVYVNAGTSGGWQIRQVR
                     LVGEAVGRKIRLEFLLQTDAFNLLEGWYLDDVTITSN"
     gene            complement(8385..10178)
                     /locus_tag="PKK20_01160"
     CDS             complement(8385..10178)
                     /locus_tag="PKK20_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNU98531.1"
                     /translation="MKGCYQRLIGIGCVWAASLLRTAGAAEPVWNIRDIPIERVSVVA
                     HRGAGFLASENTLEALELAWGMGCIPEVDVRTTKDGHVMMFHDKDFSRILPNAPDAVR
                     KRRLEDLTYEEAKQLDIGAFREPQFAGQRIVSLAEIVRALERDSRRSVYLDVKNVDFA
                     VMAKVTEGVHDQVVLATGVEADLARWKAVAPRSDTFLWIGTWGDTNDAKVEKRFAALR
                     ESDFAHIDRLQVHCHFDTNTGAMVPSDDFIRRAGVELRRHGIEFQTMPWNVPERPDAY
                     VRLLDLGSAGFGTDRPDVAMQTIREYYRQGRADWNVRDHIPLGKVVVQAHRGAGDLAP
                     EGSLEAFELGWKLGCIPEADLRQTKDGVIVSFHDASFARILPDAPEELKRKGVEDLTF
                     EEARQLDVGGFRDSKFKGQRMISLAEMVQRLKAHPHRALYIDIKRVDFRRLAAETIEV
                     HRQLILASTKHDELKLWRQLAPRSRTLHWMGGPRNWTSPSTDPTADPQARLAQRLEAL
                     RAVRFAGIDQLQIHVNTSPEGVFFPSEAFLRETGRELREHGILFQTLPWGQNDLRVFH
                     RLLDLGCASFATDFPDAAIEAIRQYYGRPRD"
     gene            complement(10646..11815)
                     /locus_tag="PKK20_01165"
     CDS             complement(10646..11815)
                     /locus_tag="PKK20_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU98532.1"
                     /translation="MTRRETLTPRERVLKALNHETPDRVPIDLGGNQTGIHRNAYRNL
                     IAHLGIQDEVRLMDAVQQLAQPCEALLTRLHVDTRYIAAGAASSWKGGIVETQRDGKR
                     WADLTDEFGVRWSMPEDAPYYMDITLHPLAQATIKDLPGYPWPKGDDPTRFAGLRDRA
                     LALRRDTPYAVVSGISGVVYETCWYLRGLEQWFCDLMTNTEFCAALLDQTLRFWLDWF
                     RVFLDEVGDLVDVIMIGDDLAGQNGPLFSPALYRRLVKPRQKQLVHSIRSRTNAKIWY
                     HTCGACTEFIPDIIDNGAHILNPVQISARGMNPADLKRRFGDRIVFWGGGVDAQRILP
                     RGTPDEVAADVRRNLEAFMPGGGYVFNNVHNIQGEVPPENVLALFDTAWEFGFYG"
     gene            complement(11825..12454)
                     /locus_tag="PKK20_01170"
     CDS             complement(11825..12454)
                     /locus_tag="PKK20_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU98533.1"
                     /translation="MKSIILALVATATFTAGLFAADSEEGFVSLMDGKTFNGWKKAVE
                     NPDTWKIQDGAFVAAGPRCHLFYVGDEKPFVNFHLKVEVMTEPGSNGGIYFHTQYQEQ
                     DWPRAGFESQVNVTHSDWIKTGSLYGLVNIGRAAAPDKQWWTQEIIVKGKSITVLVNG
                     ERVFEYNEPAGAQAGQPFARKLDKGTFALQAHDPKSIIRYRNIRVKRLD"
     gene            complement(12511..13290)
                     /gene="moeB"
                     /locus_tag="PKK20_01175"
     CDS             complement(12511..13290)
                     /gene="moeB"
                     /locus_tag="PKK20_01175"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNU98534.1"
                     /translation="MPLSDEEMRRYARQLILPEIDLAGQHTLRASSVLCVGAGGLGSP
                     VALYLAAAGIGRLGIVDADVVDCSNLQRQILHGTGDVGRAKTDSAADALRRLNPHVDV
                     VRHAVGLTRDNASQLLEGWDVVVGATDNLDARYWINEAAVRLAKPNVYGAIGRFDGQA
                     SVFAPHRGGPCYRCLYPEPPVPGPELGAASNGVLGVVPGIVGCIQAAEALKWILGIGS
                     SLLGRLLVLNALEMRFREVQIRPDPACPVCGKHSAGGRATG"
     gene            13527..14807
                     /gene="argA"
                     /locus_tag="PKK20_01180"
     CDS             13527..14807
                     /gene="argA"
                     /locus_tag="PKK20_01180"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNU98535.1"
                     /translation="MKPTDLRGILQYIPRFRERTFVISVDGAIVTDENFANILMDVAV
                     LRSLNIRVVLVHGASAQICLLASQQQVQPSNLDGSGVTDAETLKLALTAANRLTHEIL
                     EGLATQDLRAACPNAIVAHPMGILQGVDHLFTGKVERIDIDLIQTLLEQDVIPIIPPL
                     GFDGEGRTYRVNSDAIAVAVAEALKAIKLIFVTTYDGLFQQGRLFRQILASDLDRILA
                     EGREQLPAETLSKAIHAVAACRVGVQRVHVINGRLDEALLTEVFSNEGVGTLVYANEY
                     QHIRRAMKKDIPSILKLIQPSVKSEELVKRSRSSIEKTLGDYYIFEIDKNPVACVALH
                     IHPESGKGELACLYVNPSHENQGIGRKMLQFIEDKARELGLRELIALSTQAFTYFQSK
                     GGFQEGSPEDLPPGRREKYNQNGRRSKVLVKTLR"
     gene            14927..15631
                     /locus_tag="PKK20_01185"
     CDS             14927..15631
                     /locus_tag="PKK20_01185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNU98536.1"
                     /translation="MLLPLIAALPRCAISAPSLPAGMILACRRLGIRLTRHLLLVRVQ
                     AQQMEWIELDSAAQAIPTHRPLLPGTARRRMRYRISTSRFGIGQVMDSNRTPLGLHRI
                     AHKAGGGWPVGVVLVNREPVGFTWQGQPDAAIAHRVLWLEGLEPGWNRGGKVDTLERC
                     IYVHGVGNELTLGRPASRGCVHLAARDLMPLYDRLPVGTLVWIAGYPDDRFFWVASCR
                     HSGVDHFHSRWHRRQP"
     gene            15847..18441
                     /locus_tag="PKK20_01190"
     CDS             15847..18441
                     /locus_tag="PKK20_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU98537.1"
                     /translation="MSPAVGGQFKRITIDGDFGDWAGVAPAVVDAEADSGSLDFREVY
                     VANDENYLYIRVKLYTQINYSAFQHHVFIDADANPGTGLSIGGIGSELMIENGGGYQQ
                     KNGGWNEGSASNLGWAAAPSGPTTEFEVRISRQVRDAGGELVFASNSILLAVEAEDSN
                     WDTVDMAPDSGGAAYDFAPTPPKATGSTTLVSLDGTVWRYSDDGTDWGTDWLAFDFDD
                     ILYGWSSGPGLFGFGVPAGTYPTLATTLASGRNTYYLRVPFTWDYDSLGIALVANLYL
                     SDGAVLYLNGAEVKRVRMPDGEIGYGTPATGGPAAPGQAEFFTVPASGLVTGANVLEA
                     EVHQAAGTPTELAFGLKLEAIDSLPPAIEDPTQPADRTVTEGEATTFGVGIVTGTTPL
                     AYQWFKDDAPIEGATEPTLTIPVVLVTDAGGYRVEITNATGVKAVSRTAQLTTTAVAV
                     SLTNGAEPADRTVLQGESTTFAVEVIGSPVLAYQWFKNQAPIEGATGPEYTIGEAVLG
                     DAGQYHVTVSNRLGAVSSRTARLGVLPDTYPPLVAQLAGSGRKVVAVFSEPVEAASAT
                     APGHYTLSGGASVQAAALDPADRRTVTLTTTALSFGAVYTLEVKGVEDLFGNAADTTA
                     VFRSAILIDGSFEDWAEMQPLATEVQETAEGLEFNEIYVANDEIYLYLRFNFHANVGQ
                     LPVDAYFHIFSDTDNDLGTGFVTAGLGSEMMIENGWGYQQKGGQFNEGNVSGLDFVIG
                     PAAASSDFECRISRQATYDSDGQRVYDNETIALALQLISNTWASLDIAPSAGALVYAF
                     AELPAITPGPLHVQMLGSRIEIVWTGPGVLEAKDSLIEGAWAPVPDAASPYSTNATAP
                     QRYYRLRL"
BASE COUNT         3294 a         5947 c         6054 g         3320 t
ORIGIN      
        1 gctctgaagg agtggaaggg ttcgagcggg ttcaccaaga ccctgttgtt tctcggtctc
       61 ctgatgttga tcttctcgac cgttgtggtc gggctcggca actacctcgg cgtcgcccct
      121 gcaagccctt gagcaatcct atgagcactg cgtatcacgt ggtcgtctgc gccagcctgg
      181 tgcccgaccc gctccaaacc ctcgaaccgg tcgccgggcc cgccggaccc gcgctgaaga
      241 acgaggcgat gctcccattc gtcctggacc cttgggccgc acacgccctc tacgaggcgg
      301 cgcacctcgc caagaaccgg ccaggcacca aggtctggct cgtcagcctc ggcccgaaac
      361 caaagctgca acaggtcatg atgaccgtcg cccaaaaggt gccgttcgag ttcgtgcccc
      421 tggacggctc ggccagcggg ttcgccgaag cctcggccgt cgccgcagca ctcgccgaag
      481 gcatccaggc cattcccgga ctcgaccgtt cgaagctgct cgtgtttggc ggatgggaat
      541 ccgcgtcccg cggggccggc gtgacgttgt cgatggtcgg cgaacgtctt ggcatcatcg
      601 accaattcca gggcgtcgat gaactcaaga ttcaggatga cggggccttc gaggtggtcg
      661 agcggcttga aggcggacag caccagattt cgcgttgtgc cggcccgccc gccctcttcg
      721 gctgggccac gggccatctc cccgagccga agaacaatcc gcagattggc atgatcaaca
      781 tgcgcacgct gatgcccgcc ctccaaaagg ccaagccgat tcaactggcg gacgcggggc
      841 tcagcttcga cagcgttgtt ttgccgaaac aattgcgcga aacgcgcatc gtcaaggacg
      901 cctccgcgga ggacgtcgcc agagagattg tcgattggat ttcgaagtga ggtgcatcat
      961 ggagactatc ctgtttcttg cggccaccga gcctgacggc tcactcgccc cagccgctct
     1021 cgaggcgctg ggcgctgcgc gttcgctcgc tgccgccttg gccggctgta gcctgacggt
     1081 cggccttcag gggccatcgg tccaaacagc cgccgatcgg atcgccgggt gcggcgcaac
     1141 gcggttcctg ggtgtggccg gcccggactt cgggtcggcc cgttatgcga gcgacgcgct
     1201 ggcggccgag gcgcttgtcc gcgccagcca ggccacggtg gtcgtcgcgc cgggcactag
     1261 ccgcttgtcg cgctgcctgc ctggcgtcgc tcaacgcacc ggaggacagg cggacaccca
     1321 tgtcaccgcc ctgggcgccc gcgagagccg acctttcgtg aaccgctggt actatcggca
     1381 gcgcatggag gctgtcgtcc agcggaccca acgaccctgg attgtcctca tcgatcccgg
     1441 cagccagtcg ccctgggacg gcccgccggg cggcgctgtc gcggtcgaga tgatcgacgt
     1501 gaccctgccc gaggccgccc gaaaaacgag cgtcgtcgga gtccgggcgc cggcggccga
     1561 tgcccagacc atccggccgg acgccgacct tctgttcgtc gccggtgcgg gctggaccaa
     1621 gaaacaggcc gacggccagg cgcgcgtggt cgaggcggaa cagatcatcc tcgagtttct
     1681 caagcgcagc cgcgcctcgc taggcagcac caagtcgttg gtcgatctgg gcggcgaggg
     1741 ccagaccgtc ctgtcgtttc tctcgcacct gaaccagatt ggccagacgg gcgcgacgcc
     1801 acggcatccc aaggggctct cgacctgctg tcacggcgag gaaccccaca ccgtcggctg
     1861 gcgtttcatc aacgagcgcc gggccatcag cctcgatccg aactgcgggt gggcgcgggg
     1921 caaggccgac gtgctttatg ttgccgacgc ctttgccgtg atgcgagagg tcaaccgcct
     1981 gttggcggcc aatgcctgaa aacccgagga cgtgaagcgc gccatgcgcc tttgcgttgt
     2041 gttcaatccg accgcgcgcg gcaacaatgc caagcgcttc cgtcgacgac tcgaggcccg
     2101 ggcgcgcgac tgtgtcctga tgccgaccac cggtcctgga gcggccgtcg atctggctgc
     2161 cgaggccgtg cgccagggat ttgacaccgt ggtcgcggcg ggcggcgacg gaacggtcaa
     2221 cgaagtgctc aatggcatcg ccggcgtgga acacggcctc gaacggacgc gcctgggcat
     2281 cctgccgctg gggaccatga atgtcttcgc acgggaactg ggcttgccgt tgaaccccga
     2341 cgccgcctgg tgtctgctcc gcgagagcaa tgaaaccgct atcgacctgc cctgggcgga
     2401 attcgaggtc gaggggcgaa caacccgtcg ctgcttcgca caactcgccg gggcgggtct
     2461 ggactcgcgc gccatcgggc ttgtcgactg gacctggaag acgcggatcg gttctttggc
     2521 ctatgggatc gccggtttgc gggcaatgcg gggccctcaa ccgctggtga cagtgacgct
     2581 tccggacggc acgacatcga gcggggagtt ggtgctggtg ggcaacggga ccctctacgg
     2641 cgggtgtgtg acgatgttcc ctggcgccag tctgcgcgac ggccaactcg cgattcgggt
     2701 gctgccccgg atcggagtcg tcaccatggc caggttcgcc ctctcctggc tgttccgggg
     2761 cggattcgcc gtgccgggcg aacggcatct gcgtgcggac cggttcacgc tcacaagcgg
     2821 cgaggccgtt ccgtttcagg tggacggcga caatgctggt ccgctcccgg cccgcttctc
     2881 ggtccgtccg cgcgctcttc gagtggtcgc gccttgaagc cccgcgatgg gcatcggacg
     2941 actcgctctc cagatggcaa gcacagcctc ttgctcaccc cgcgtccgcc ccggcgcttg
     3001 ggtgcggtgg gtctgctcaa ggccatgcgc caatgcccgc acgatttccc tgccatgcgc
     3061 ggttcgagct acgacgccat tctcgaacct gccgtaaaag gcgcccgcaa ggaaaacggc
     3121 ccatggctgc tgctgtgcct gtccacttcg ccatgctatg gcttgtcctc accggggcgc
     3181 ccgatcggga ccgccgcgct tgtcgctgat gcggaagaaa tcaaagtccg cgaagccccc
     3241 gggcgctttg gtcgcgtagc aaaacaaccc aaaccggtag cccatgaaat ggggaatcgt
     3301 gtagctcatt ttcaactcgt ttccaatcgc cgtccacgat gtgccgtcga ggctgtaaca
     3361 gaaccgcgcc gtgtcggtcc ggttccggaa atcgcactcc gtcttcagga acaccgtttg
     3421 cgaaggcaca gcgacgcgct cgatctcgat cggcgcattg gactgggcgt tgaccatgac
     3481 gacgaaggtg gcgccggctt ccgacgtcag gcccacgaat ccgtagcgat gctgcaacag
     3541 ggccagcccc gcatagtcgc catccttgat gttgcgcgcg tcgaccgagg tcgtgcctga
     3601 acacgcgggg ccgatggtcc gttgggtaag ggtgtttcgc gcggcgagga gatgggcgtc
     3661 agtgcggccc gtggtcaggc ggaggaagcc cgggcgttgg gagaccgacc agagcgtgtt
     3721 gtcgggattg tggttccact gccagaccag cggcaaagct ggttcacccg ctgcgcgatc
     3781 gaattcatcc gaagccacga tgccgggaat gagcccccgg ctcgggggca ggtccaaccg
     3841 gtccggcgcc ttgccttcga tgccgaatac cggccagcca tctttccatt ggacggggac
     3901 gaggtagggg attcggccga cggccccgaa atcccggaac aggtaggcga accagtcgcc
     3961 gccaggcgtg tcgatgaggc tgccctgcgc gacgcccttg tcctgcagcg caacgcggcc
     4021 cacgtagggg ccggcgatgg tgttggcgcg gtggacgatg acggttcgca ttccgccgcg
     4081 cggccagacg atgttgaaca ggtagtaggt gttgttgacc ttcaggagtt gggagccttc
     4141 cgccggaagg ccgatggcgg cgccggcgac ggcgctggcg tttgtgacga ccacctggtt
     4201 gaagccaccc tgcttcaaac ccgagaaatc cgggttcaac tcgacaagcc ggagatcgcc
     4261 gccgccgtag agcatgtaga cgcggccgtc atcgtcgaag aagagcgagt ggtcgtgaag
     4321 catcggtctg aacgaagtcg ccttccacgg ccctttctcg atgtccggcg tggtgtagac
     4381 gtgggtcttg ccggtggttt gggagaacgt ggtgaccaca taggtgccgt cgtggtgccg
     4441 gatgctgctg gcccaggaac cgccgccata ggcgctcttg ccgttgttga gattcagcgc
     4501 gtcaacgtcc tcgagcgtgt cgtaggcgta gctgaccagc gtccagttca cgaggtcttt
     4561 ggacttcatg atgggcagcc ccggactcat gtgcatggtc gtgctgctca tgtagtaggt
     4621 gtcgcccacg cgtatcatcg ccatgtccgg cacgtccgca tggataatgg gattccgggc
     4681 gaaagcttcc ggatcccctg cgggcgctcg aggcgcggcg agacacccgc acaccagcgc
     4741 gaacaccgcg ggcgagaaca ctcgctgact catcgcatca caagattggt gagttcctcg
     4801 aatcagttgg acgtgatcgt gacgtcgtcg aggtaccagc cctcaagcag attgaaggcg
     4861 tcggtctgaa gcaggaactc gagtcggatc ttgcgtccga ccgcttctcc gaccagacgg
     4921 acctgccgaa tctgccagcc gcccgaagtg ccggcgttga catagaccgc gtcgctcacc
     4981 gtggccaggg tcgaggcatc gagaacgttg atcatgacct gatgggcctg ctgttcgacc
     5041 ggcacgccgg caaggaaatc gaggttgagc cactcggcga atcgcaacgt ggcggagctg
     5101 acgcccgtga gatcgatctc cggcgactgg agccagcagt aggagtatgc gcccacattc
     5161 cggttcagac cggtgccata cacgtttgcc ccgctgtaag ccgcggtcgg gccaaccgtg
     5221 ggcgcgccgc gttcccagcg gtcctcgttg cccgcatgag tccaacccgg cacagcctgc
     5281 tcgaagtcgt cgaaaaagag cggcggcggc ggcagggcga cggcccggaa aaagccggct
     5341 ggggcggttc cgacggggat cgaggcggag aagagcccct ggccgagatc gacgaatgtg
     5401 aggttcggca cgacgagcca ggcgggatcg gacagggcgg ctttggactc gatctgtgcg
     5461 gggcgattgt tgaagggcgt gtagaactcg agggcgacgg cctcagccgc cggtttgatc
     5521 gccgtgatgg tcagcggtct gaactcctga ttgagaggct tgccctgctg gccgttgaca
     5581 tagatgccga gaatgtcgct ggggctcagg gcgcgctgcc agatccccag gtcatcgatg
     5641 ctgccgacgt agggacgatt gatggtcaga ccggcggcgt agttgccgat aaccagcggg
     5701 tcggcggtga gttcgccaag gcgcccggaa aggagcgcgc cgagcggctc gccattccga
     5761 taccactgaa cggtcccccc ccgatacaca taggcgaagt gggtccagat gccgagttcc
     5821 cacgattgcg ccgggccgtc gtagggagcc ttgccaatcc cgtaggtgcg cacaacgccg
     5881 ccgggcagga gcacaaagtc gaagttggtg cccttgcgga tgacccgatc gtggttggcc
     5941 ggggacatgc cgtccacctt gagccaaacg ctgaccgtgg cctcggtggt cagatcgagc
     6001 atcggatcat gcccgacaac gatgtagtcg tccgatccgt cgaatcggaa ggcgccgccg
     6061 atgggcccgg cgatgcgcga cgcgtcgtcc atgttgacca attggccatg gaagttgttg
     6121 ccgctcgaat cggtgagcgt ggttccaggg ccgtcatcga acgtgtaata cgccacgagc
     6181 ccgctggtga tcgcatcgag cgcctcgact tcgagcagag cggcctcgct ggtcgtcatc
     6241 ccctcgctgt tggtgacgcg aaccgcatag cccccggcat cggcgggctg gagccgattc
     6301 agggtcagag tgttttgcac cgcgccggcg atcgggatgc cgttcttgag ccactggtac
     6361 tcaagcgggc gtttccccgt gacaaagacc tcgaacatgg cggtcgtccc ctcgatccgc
     6421 ttctgcccgg caggttgttg cacgatgacc ggcacgccga cgacatcctg ccccgccagt
     6481 tccagaatct cggcctccga gagcgggcgc gaccagattc ccaactcgtc caaacgccct
     6541 tcgagtcggc tggcgctcgt cacggtggca aggtcgtcat aggcgccgat gacaaggccg
     6601 gcctcggcgg gcggcccgag agtgccgggg acgggatcgc ccacgcggaa tccgttgcgg
     6661 aagaaactga cagtgttgtt gcggtagagg accacccagt gctgccactg atcgagggcg
     6721 accgatccct gcggcgcgtt cacctcgaag gcgctccgca cgaccccgcc ggcgtcggct
     6781 cccctgcccg cgcggctgac cagggtctgc acaaccgtgc ccgggtcgtt gatgagacga
     6841 atgccgaact gatcggcttt gcgcagaatg taagaagcgc tgcgtgtgta gacgcccaca
     6901 tcctcaggca cgccccagcc gagcgggtga atccaaaagg caaaagtcgc ctcgccctgg
     6961 acctggctga gcgactggct ttgcgggacg gagaccaggc tggcgacgcc gtcgaagctg
     7021 agactgccgt tgacccggcc ggcgctccag tgggacgtct catcccagta cccggacaac
     7081 tggccgttgt tgccattgcc ggaggcgtcg gaagccgtca acccggacgt ttcattgaag
     7141 tgcatcgaga ggacgcgcgc ggtttgcagg ccttcgacgg agcggacttc gaggctggcg
     7201 gactggctct tggcggttcc gatgtcgttg ctgacctcga cccggtagag cccggcgtcg
     7261 gcgggcgtcg cgtattcgac aaccagggtt tcggccgtct gggtgcggag tggctgatcg
     7321 ttgcggtacc agaggtagcg cagaggattc tggcccgcgg cgcttaccga gaaagtggcc
     7381 gtaccgccgg cgtacacgct ctgggcctgg ggctggccgg tgacggtcgg cgcccccacg
     7441 cgggcggcga cctgcagaat ctcctccgcg gtgagcgggc ggttccagat cagcacctgg
     7501 tcgatggtcc cgttgaagac tcgattgaga tcatcgcccc cgatcaccag agcgagatcc
     7561 gtcacgtcga tcggcgccgc cacggcccgc ctgcctttga gggcgccatc cagataaacg
     7621 agggcctcgg ttcccgtgaa cacgccggcg acgtgatacc acgtgtctgc cgacaaggcg
     7681 tcgacgatgc cgacggtctg attgacggtc cctttcttca acaggaagtt catgccgccg
     7741 ccttcggtcg ccacgccctg gagaagaaag aacatgttgc ctttctcgag cagggcgtag
     7801 gtgctcgcgt tcgacgccag cggaacgttg gacctgaacc aggccgacac tgtgaatccg
     7861 ttggccaaat ccgccgcaat gctcgcatgg cttggcacaa ccaggcggtt cgatgtcgcg
     7921 ccggtgaagg cgaggccgcc gccgaatcgc cccgccaccc attgcgcgtc gtcggtgtaa
     7981 ttgtccagag tgccgtgatt cgcgttcggt cccgatgtgt cgtgggcgat ggctccgctg
     8041 ccctcctcga agtcccaacg cgccacaaga ccgtccagcg ggattgtctg cgccgcgagc
     8101 ctcgaggtca cgctcagccc gcaaccagcc aggagtgcgc ccaagaccag acgccgcatc
     8161 ggttgacgtg tgcttttcat agtgtcgctt aatgtgtgtt gacgaaacaa cccctgttcc
     8221 cgcctcgctg tcggcgtcgg tgagcatcag tgtcaccgag cgaacaccca ggacgggtga
     8281 gctgttcacg ttacggaacg tcacggcgcg ccagagatgg gagtggcctc gcatctgacc
     8341 cggcggcact ctcgcaaaaa ttggaatgaa ttgttgcaca gacctcagtc gcgcggccgc
     8401 ccataatact ggcggatggc ctcgatcgcg gcgtccggga agtcggtggc gaaactggcg
     8461 cagcccagat cgagcagacg gtgaaagact cggagatcat tctggcccca cggcagggtc
     8521 tgaaacagta tgccgtgctc gcgcagttcg cgtccggtct cgcgcagaaa agcctcgctc
     8581 gggaagaaga caccttcggg gctggtgttg acatgaatct ggagctgatc gatgccggcg
     8641 aaacggacgg cgcgcagggc ttcgaggcgt tgggcgagcc gggcctgcgg gtcggccgtc
     8701 ggatccgtcg aagggctcgt ccagttgcgc ggaccgccca tccagtggag tgtccgcgac
     8761 ctgggggcga gctggcgcca gagcttgagt tcgtcgtgct tcgtgctggc aaggatcaac
     8821 tggcgatgca cctcgatggt ctcggcggca agccgcctga aatcgactct cttgatgtcg
     8881 atgtagaggg cccggtgcgg atgggctttg agccgttgga ccatctcggc caggctgatc
     8941 atccgttgtc ctttgaattt gctgtctcga aaaccgccga catcgagctg cctggcctcc
     9001 tcgaatgtga gatcctcaac gcccttcctc ttcagttcct ccggcgcgtc gggcaggatt
     9061 cgcgcgaagc tcgcatcgtg gaacgagacg atcacgccgt ccttggtctg ccgcagatcg
     9121 gcttccggga tgcagcccag tttccacccc agctcgaagg cttcgagcga gccttcgggc
     9181 gccaggtcgc ccgcaccgcg atgcgcctgc acaaccacct tgccgagcgg gatgtggtcg
     9241 cgcacgttcc agtccgcccg cccctgccgg taatactcgc gaatcgtctg catggcgaca
     9301 tcgggtcggt cagtgccgaa tccggccgac ccgaggtcga ggagccggac gtaggcgtcg
     9361 ggccgctcgg ggacattcca gggcatggtc tggaactcga tgccgtgccg gcgcaactcg
     9421 acgcccgcgc ggcggatgaa gtcgtcgctc ggcaccatgg cgcctgtgtt ggtgtcgaag
     9481 tggcaatgca cctggagccg atcgatgtgg gcgaagtccg attcccggag cgcggcgaag
     9541 cgcttctcga ccttggcgtc gttggtgtcg ccccatgtgc ctatccacag gaaggtatcg
     9601 ctgcgcggcg caaccgcctt ccagcgggcc aggtcggcct ccacgccggt cgccagcacc
     9661 acctggtcgt gcacgccctc ggtcactttc gccatgaccg cgaaatccac gttcttgaca
     9721 tcgagataga ccgatcgtct cgaatcccgc tcgagcgccc tgacgatctc cgccaggctg
     9781 acgatccgct gtccggcgaa ttgcggttcg cggaatgcgc cgatgtcgag ctgtttggcc
     9841 tcctcatacg tcaggtcctc gagcctgcgc ttgcgcacgg cgtcgggcgc gtttggcaga
     9901 atccgggaga aatccttgtc gtggaacatc atcacgtggc catccttcgt ggtccgcacg
     9961 tccacctcgg gaatgcagcc catgccccag gcaagttcga gcgcctcgag ggtgttctcc
    10021 gaggccagaa atccggcgcc ccggtgcgcg accacggaaa ccctctcgat cggaatgtcc
    10081 cggatgttcc aaaccggctc cgccgccccc gccgtacgca ggagcgacgc agcccaaacg
    10141 catccaatcc cgatcagtct ctgataacag cccttcatcc ggtcagtcct tgcctcctcg
    10201 atccgttcgg atcgcgtccc acacccgatc ctcccgcgat cgaggctctg atgcgccact
    10261 ctgcgaactg ggagcggagg ggtcaaggcc tcactaaaag catggggtca catcttcaca
    10321 tttgacaatt gcccgcgtgc cgcgccgatc cggccccgcc aggtcttgcg ccgggggatc
    10381 tccgggcgaa cctgggctgc cggcctggcc catgctcggc aaaatgtcaa ttatgaagat
    10441 gtgacccctg atttgccgcg ggcgcgcagg gtgcagagcg caaaggagaa gatcaatatg
    10501 gcagcccccc gctcacccga tcacgtctgt tggttcccag gcggtcgttg ggctggaggc
    10561 gcgactccaa gcttcactgc gcgagtgcca tttccgcaaa ggatcgggca agagccggcg
    10621 tgcgaggctc aaatcgcccg tccaatcacc cgtagaaacc gaattcccaa gcggtgtcga
    10681 agagcgcgag cacgttctcg ggcggcacct cgccctggat gttgtgcacg ttgttgaaga
    10741 cgtagccgcc tccaggcatg aaagcttcga gattccgacg gacatcggcc gcaacctcgt
    10801 cgggcgtgcc gcgcggcaga atccgctgcg cgtcgacccc accgccccaa aacacgatcc
    10861 ggtcgccaaa gcgccgcttg aggtcggccg gattcatgcc gcgcgcgctg atctggactg
    10921 gattgaggat gtgggcgccg ttgtcgatga tgtccggaat gaactcggtg caggcgccgc
    10981 aggtgtgata ccagatcttg gcgttcgtgc gcgagcggat ggagtggacg agttgtttct
    11041 ggcgcggttt caccaggcgg cggtagaggg ccgggctgaa caacgggcca ttctgcccag
    11101 ccagatcgtc tccgatcatg atcacgtcga ccagatcgcc gacttcatcg aggaagacgc
    11161 ggaaccaatc gagccagaat cggagcgtct gatcgaggag ggccgcgcag aactccgtgt
    11221 tcgtcatcag atcgcagaac cattgttcga gtccgcgcag gtaccagcac gtttcgtaga
    11281 ccacgcccga gatgccgctc acaacggcgt agggcgtgtc gcgccgcaac gccaacgcgc
    11341 ggtcgcgcaa cccggcaaac cgcgtcgggt cgtccccttt gggccagggg tatccgggca
    11401 gatccttgat tgtggcctgg gcgagcggat gcagcgtgat gtccatgtag tagggcgcat
    11461 cctcgggcat cgaccaccgg acgccgaact cgtccgtcaa gtccgcccaa cgcttcccgt
    11521 cgcgctgggt ctcgacgatg ccgcccttcc aactcgatgc cgccccggcg gcgatgtagc
    11581 gcgtgtcgac gtgcaaccgg gtgagcaggg cctcgcacgg ttgcgccagt tgctggacgg
    11641 cgtccatcag acgcacctcg tcctggatcc ccaaatgggc gatcaggttg cggtacgcgt
    11701 tgcgatggat gccggtctga ttccccccga gatcgatcgg cacgcgatcc ggggtctcgt
    11761 ggttcaacgc cttgagaacc cgctcgcgag gcgtgagggt ctctcgcctg gtcatgggcg
    11821 gggctcaatc cagccgcttc acccggatgt tgcggtagcg gatgatgctc ttcgggtcgt
    11881 gggcttggag ggcgaaggtg cccttgtcca gcttgcgggc gaaaggctgg ccggcctgcg
    11941 ccccggcggg ttcgttgtac tcgaagacgc gctcgccatt caccagcacg gtgatcgatt
    12001 tccctttgac gatgatctcc tgcgtccacc attgcttgtc gggagccgcc gcccgcccga
    12061 tgttcacaag tccgtacagg ctgcccgtct tgatccaatc gctgtgggtc acgttgacct
    12121 gcgactcgaa tccggcccgg ggccaatcct gttcctggta ctgcgtgtgg aagtagatgc
    12181 cgccgttcga gcccggctcg gtcatcacct cgactttcaa gtggaaattc acaaacggtt
    12241 tctcgtcccc gacatagaac aaatggcagc gcggcccggc ggcgacgaac gcgccgtcct
    12301 ggattttcca ggtgtcggga ttctcgaccg ccttcttcca cccgttgaag gtcttcccat
    12361 ccatgagcga aacaaagcct tcttccgagt cggcggcgaa aaggccggcg gtgaatgtcg
    12421 cggttgcaac cagggcaaga atgatcgatt tcatggcgac agcatgacga aaagaccggc
    12481 cttgccaagc gggaattggc aatcgagcga ttagccggtc gctcgaccgc ccgcagaatg
    12541 cttgccgcag accggacacg ccgggtcggg acggatctgg acttcgcgaa accgcatctc
    12601 gagggcgttg aggaccaaca ggcgtccaag cagcgaagag ccgatgccga gaatccactt
    12661 gagcgcctcg gccgcctgga tgcagccgac gatcccaggc accactccca ggactccatt
    12721 ggatgccgcc cccaactcag gtccgggaac gggcggttcg gggtacagac agcgatagca
    12781 gggcccgccc cgatgcggag cgaacacgct ggcctgtccg tcgaatcgtc cgatcgcgcc
    12841 gtagacattc ggtttcgcaa gccgcacggc ggcctcgttg atccaatacc gtgcgtcgag
    12901 attgtcggtg gctccgacaa ccacgtccca cccctcgagc aactgcgacg cgttgtcccg
    12961 cgtcagccca accgcgtggc gcacgacatc cacgtgcgga ttcagacggc gcaaggcgtc
    13021 ggcggccgag tcggtcttgg cccgccccac atcgccggtc ccgtgcagga tttggcgctg
    13081 gagattcgaa caatccacga cgtcggcatc cacaatcccc agccgtccaa tgccggccgc
    13141 cgccagatag agcgcgactg gcgacccgag cccgcccgcg ccgacacaca agacgctcga
    13201 tgcgcgcagc gtgtgctgtc cggccaaatc gatctccggc aggatgagct gccgcgcgta
    13261 ccggcgcatt tcctcgtcgc tgagcggcat ggtcattttg gtcgacggcg aagatggctc
    13321 ggacccgggc attgcacaag gccaaccagc ccggttgcgg ttcatcgcga ggcgtgtgaa
    13381 atcaccaggc tgcggcaagc gccgctctgc ggcgcggggg ggcgatcgga tcgcctcgtc
    13441 ggaggagccc ggcagaaatt cattgaatct gcgtcgcgag tgcctaccta ttggcggcgt
    13501 cgcaacgaac acatgcgggc gtcagagtga aaccaaccga tctgcggggc atcctgcaat
    13561 acattccgcg ctttcgcgaa cggacgttcg tgatcagcgt cgatggggcc atcgtcacag
    13621 acgagaactt cgccaacatc ctgatggatg tggccgtgct gcggtccttg aatattcgcg
    13681 tcgtgctggt gcacggcgcg tcggcccaaa tttgcctgct cgccagccaa cagcaggttc
    13741 agccgtcgaa tctcgatggc agcggggtga cggatgccga gacgctcaag ctggcgctca
    13801 cggccgccaa caggctcacc cacgaaatcc tcgagggctt ggcgactcag gacctgcggg
    13861 ccgcctgccc gaacgccatc gtggcccatc ccatgggcat cctccagggc gtcgatcacc
    13921 tcttcacagg gaaggtggag cggatcgaca tcgatctgat ccaaaccctg ctcgaacagg
    13981 acgtgatccc catcatcccg cctctcggtt tcgacggcga aggccgcacc taccgggtga
    14041 actccgacgc catcgcggtg gcggtcgccg aggcgctgaa agcgatcaaa ctgatctttg
    14101 tgaccacgta tgacggcctg ttccaacaag gccgcctctt ccggcaaatc ctggccagcg
    14161 acctcgaccg cattctggcc gaaggccgcg agcagttgcc ggcggaaaca ctctcgaaag
    14221 ccatccacgc cgtggcggcc tgtcgcgtcg gcgtccaacg cgtgcacgtg atcaatgggc
    14281 ggctcgacga ggcgttgctg accgaggtct tctcgaacga aggggtgggc acgctcgtct
    14341 acgccaacga ataccagcac atccgccgcg cgatgaagaa ggacatcccc agcatcctga
    14401 aactcatcca gccctcggtc aagtccgagg agttggtgaa gcggagccgg agcagcatcg
    14461 agaaaacgct cggcgactac tacattttcg agatcgacaa gaacccggtc gcctgtgtag
    14521 ccctgcatat tcaccccgag tcgggcaagg gagagctggc ctgtctgtat gtgaatccct
    14581 cgcacgagaa tcagggcatc gggcgcaaga tgctgcagtt catcgaggac aaggcgcgcg
    14641 agttgggcct gcgcgagttg atcgccctgt ccacccaggc cttcacctat ttccagtcca
    14701 aaggcggatt ccaggaaggc tcgcccgaag atctgccgcc gggtcggcgc gagaagtaca
    14761 accagaacgg acggcgctcg aaggtcttgg tcaagaccct gaggtgagaa ccgcgccgcc
    14821 cagcccgcga cgaatctcgg agcaccgatc cagcggttgc tggagagtcc ctctctggga
    14881 atcgcgcagt cagcagtcca gagcctctcc cgccgccgcc ggatcggtgc tcctgccttt
    14941 gatcgcggcc ctgccgcgct gcgcgatctc cgcgccaagc ctgcccgcgg ggatgatcct
    15001 ggcctgccgc agacttggga ttcggttgac ccgccatctc ctgcttgtgc gggtgcaggc
    15061 tcaacagatg gaatggatcg aactggattc cgcggctcag gcgatcccca ctcaccgccc
    15121 tctcctccca ggcacggcgc gccgacgcat gcggtaccga atctcgacct cgcgcttcgg
    15181 cattgggcag gtcatggact ccaaccgcac gccgctcgga ctccaccgca tcgcgcacaa
    15241 ggccggaggg ggatggccgg ttggggttgt gctcgtgaat cgggaaccag tcggcttcac
    15301 atggcaaggg cagcccgacg ccgccatcgc tcatcgggtc ctctggctcg aaggcctcga
    15361 accggggtgg aatcgcggcg ggaaagtcga cacgctcgaa cgctgcatct acgtccacgg
    15421 cgtcggaaat gaactcacct tgggccgccc cgcctcgcgc gggtgcgttc acctggcggc
    15481 acgcgatctg atgccgctct atgatcggct gcccgtgggg acgctggtgt ggatcgccgg
    15541 ctaccctgac gatcgctttt tctgggtcgc gagttgccgc cactcgggtg ttgaccattt
    15601 ccatagtcgg tggcatcgtc ggcaaccatg aaatccctct tccacatcca tccggagtcc
    15661 ttgaaatccc aaacatgtgg tgatcaagtc aggttctgtc cccccccctc cttccaggtt
    15721 tcaaggattc cgcggagcga ggcgcgagag agagggaagg catggcggct gttcatgaag
    15781 tcacgagtcg cagatggtct gaggcgcgcc tcgctggctg ttgttgtaag cgcgagcctg
    15841 ctctggatga gccctgccgt cggcggtcag ttcaagcgga ttaccattga cggagacttc
    15901 ggagactggg caggcgtggc gcccgccgtc gtcgacgcgg aagccgactc gggatcactc
    15961 gacttccggg aggtctatgt ggccaatgac gagaattacc tgtatatccg ggtgaagctc
    16021 tacacccaga tcaactacag cgcgttccag caccacgtgt tcatcgatgc ggatgcgaat
    16081 ccggggacgg gtctgtcgat cggcggcatt ggctcggaac tgatgatcga gaacggaggc
    16141 ggctatcagc agaagaacgg aggatggaac gagggcagcg cctcgaatct cggttgggcg
    16201 gcggcaccca gcggaccgac cacggagttc gaggttcgca tctcgcggca ggtgcgcgac
    16261 gccgggggcg aactggtctt tgcctcgaac agcatcctgc tggcggtcga ggcggaggac
    16321 agcaattggg acacggtcga tatggcgcct gacagcggtg gcgcggctta tgatttcgcg
    16381 ccgactcccc cgaaagccac gggctcgaca acgttggtgt cactcgacgg cacggtctgg
    16441 cggtacagcg atgatgggac ggactggggc acagactggc tggcgttcga cttcgacgac
    16501 attctgtacg ggtggagcag cggtcctggg ctcttcggct ttggagtacc ggcgggaacc
    16561 tatccaactc ttgctacgac ccttgcttcc ggccgaaaca cgtactacct gcgtgttccg
    16621 ttcacgtggg actacgacag tctggggatt gcgttggtgg cgaatctcta tctttccgac
    16681 ggtgcggtgc tgtatctgaa cggcgcggag gtcaagcgtg tgcgcatgcc ggatggggag
    16741 atcgggtacg gcacgcccgc cacgggaggt ccggctgcgc ccggtcaggc ggagttcttc
    16801 actgtgcctg cgagcgggtt ggtcacgggg gcgaatgtcc tcgaggccga ggtccatcag
    16861 gcggccggca cgccgaccga gttggccttc ggcttgaagc tggaggcaat cgacagcctt
    16921 ccgccggcga tcgaggatcc cacccagccg gccgaccgca ccgtgacgga gggcgaggca
    16981 accacgttcg gcgttggcat cgtgaccggc accacacctc tggcctatca gtggttcaaa
    17041 gacgacgcac cgatcgaggg tgcaacggag ccgacactga ccattcccgt ggtgctggtg
    17101 accgacgcag gcgggtaccg ggtggagatt acaaacgcaa cgggggtcaa ggccgtcagc
    17161 cgcaccgccc aactgacgac cacggccgtc gcggtcagcc tgacaaacgg cgccgagccg
    17221 gcggatcgga ccgtccttca gggggaatcg accacgtttg ccgtggaggt gatcggctcg
    17281 ccggtgctcg cctatcaatg gttcaagaac caagcgccga tcgagggtgc gaccgggcct
    17341 gaatacacca tcggcgaggc ggtgctcggc gatgccggcc agtatcacgt gacagtcagc
    17401 aaccgtctgg gcgctgtcag cagccgcacg gcgcgcctgg gcgtgcttcc ggatacatat
    17461 ccgccgctgg tcgcgcagtt ggctggctcg ggccgcaagg tggtcgcggt cttctcagag
    17521 ccggtcgagg ctgcttccgc cactgcaccc ggtcactaca cgctcagcgg cggggcgagc
    17581 gtgcaagcag ccgcgctcga tccggccgac cggcgaacgg tcaccctgac gacgacggct
    17641 ctgagctttg gcgccgttta caccctggaa gtgaaagggg ttgaagacct ctttggcaat
    17701 gccgccgaca caaccgccgt tttccggtca gcgatcctga ttgacggcag cttcgaagac
    17761 tgggcggaga tgcagcccct ggcaacggag gtgcaggaga cggccgaagg gctcgagttc
    17821 aacgaaatct atgtcgccaa cgacgaaatc tacctttacc tccggttcaa tttccacgcg
    17881 aacgtgggcc aactgccggt ggacgcctac ttccacatct tctctgacac ggataacgac
    17941 ctgggaacgg gctttgtgac cgccggactc ggctcggaga tgatgatcga aaacggatgg
    18001 gggtatcagc aaaagggcgg tcagtttaac gaaggcaatg tgagcggatt ggacttcgtc
    18061 atcggacccg cagcggcctc atccgatttc gagtgccgca tctcgcgtca ggccacctac
    18121 gacagcgatg gtcagcgggt ctacgacaac gagacgatcg ccctggcgtt gcagttgatc
    18181 agcaacacgt gggcgagtct ggacatcgcg ccgtccgccg gagcgctcgt gtatgcattt
    18241 gccgagttgc ccgcgatcac gccagggcca ctccacgtgc agatgctcgg cagccggatc
    18301 gagattgtct ggaccggccc cggcgtgctc gaagccaaag acagtctgat cgagggcgcg
    18361 tgggcccccg tgccggacgc cgccagtcct tactcgacca acgcgaccgc gccgcagcgc
    18421 tactaccggc tgaggcttta gcccggatct tgtacaaggc ttcgcgggct ctcatatccg
    18481 ggctagccag aggcgacggg gacgtcgccc ctccaaggtt cggttcatgg agagcgttga
    18541 cattcgaccg gtgaatgcga acggggacca tgaaccgatt tgtagccgcc gacgtgagtc
    18601 ggcgctgatt tctgc
//
LOCUS       DAOQRO010000015        17901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2250_length_17901_cov_4.876275, whole genome shotgun sequence.
ACCESSION   DAOQRO010000015 DAOQRO010000000
VERSION     DAOQRO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17901
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_17901_cov_4.876275"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..3287)
                     /gene="glnE"
                     /locus_tag="PKK20_01195"
     CDS             complement(324..3287)
                     /gene="glnE"
                     /locus_tag="PKK20_01195"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNU98538.1"
                     /translation="MRSRTWADAIARCADPRRAEHSLGLLWATEAKPALQMAKAEAAR
                     MLAALLSGSQVSIETLIAHPDWVPALLDVKTLSTPRSMPRLRADLQRAIGPLIKRADD
                     SAAFAQLRLFKQRETLRIAIRDLSGPGKAAVITRELSDLADLCLETVCELCRRQIEGR
                     LGKPWHTDPGGGWRPTTFCVLGLGKLGGQELNYSSDVDLMFLYSDEGFGFRESPAPGE
                     TGKGVANHLYFQRLTEAIVAEVARMTPEGTLYRIDLRVRPEGDRGPLARSLAGCENYY
                     WQWGQTWERMMLIKARRVAGDAALANEFIEMIQPFRYPRSLSRQALDDVADMKQRIEN
                     EIVRAGEIDRNVKLGRGGIREIEFVAQTLQMMHAGRHPFLAHSQTLPALAKLAEYQLL
                     PTEDAQALGAAYLFLRDIEHRLQMDNNLQTHTIPTEREKRERLARLMGYDALAAFETA
                     LLDHRERVRAIYDRLLHTDAPQPSLQFPDLTAQEDQWKSLLHDLSFRDPDKALRLVRA
                     LVQGPGFGHLSARTEKFGRQLLAQFLNLCPARGVADSQDAARFPDGDPHSKRLSDPDR
                     VLARLDTFVAAYGGRALLFETWATNPSLFELIILLFDRSEFLAETAIRTPDLVDELEL
                     SGYLRRAKNAARILKDLRHGLDDPDQKLWLRRYHQTELMRIGLRDILGLADFEQNLVE
                     LSALADACLDYALEVALRKHRRRPECIAIVGLGKLGGAELNYGSDLDVVFVADPKTRD
                     LPGLQRVAVEVMDLLGSPTELGVAFEVDARLRPDGEKGLLVNTLDAYGDYYRQRARLW
                     EIQAISRSRLVAGSREVGARYRQMVATLADFSRPDTAAAPARDPDWKGEIARMRLRIE
                     QERTPKGKEHLAIKTGAGGLVDAEFIAQMLCLARGWQQPNTLEALRQARDQKALDPGA
                     AQQLIENYRRLRRIEGILRRWSYAGETELPDDPAPQYRVAVRCGFANAGDFLAAVGRY
                     RQNIRAVYRSVFGNG"
     gene            complement(3319..5505)
                     /locus_tag="PKK20_01200"
     CDS             complement(3319..5505)
                     /locus_tag="PKK20_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNU98539.1"
                     /translation="MRTILRFLLRLCFGFRAYNTDVLKTPGPVLLIPNHVSWLDWLYL
                     YVLLDLDWKCVVSNVAAQYSWVHRRIMLNRRTFPVDTNSPYAAKRMAECLERGGRLVL
                     FAEGQITRTGSLMKVYEGTGFLMHKTRARLIACYIRGANRLRWSRQPGWRRWWPTITV
                     HFSPPLAPPQRQYQSTAEARAFLSNWLRDQMLLQQFETEMAFGPKHVLDAILEAARAQ
                     PGKTVLQDATTQSLTYRRLLTGAHLVGRKLHAQLPPNTDRVGVLLPNANAMPTLLLGL
                     WSLGRVPAILNFSTGVPVMLACARLAGLEALVTARAFLAGAKLSADPFVAAGLKVIYL
                     EDVRKSVTSGERLLGLARVILAPASTLRPARGSDRPTAGGFSASDRPAAILFTSGSEG
                     APKGVELTHGNLLANIRQMLTVIDLEDRDRVFNALPCFHSFGLTVGTLLGLVRGLYVF
                     MYPSPLHYRVIPNVLYDRYCTVFLATNTFLNGYARRAHPYDYRAVRYLFAGAEKVQDE
                     TARLWMQRFGVRILEGYGATECSPVISVNLPQASRHGSAGRFLPGLEYKLESVEGVGD
                     GGRLLVRGPNVMRGYLNPDANAKFKSLGGWYDTGDVVNVDADGFVHILGRLKRFAKVS
                     GEMVSLTAIEDALAGAFPQYGVRFEVAIVARPDAEKGETIIAVTNEARLTLDEIRAVI
                     RAKGFTNLCVPREVRFLRQIPKLGTGKVSHMELSRRVQDLPPHRQA"
     gene            complement(5531..6838)
                     /locus_tag="PKK20_01205"
     CDS             complement(5531..6838)
                     /locus_tag="PKK20_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU98540.1"
                     /translation="MNRARNYRLLLASQLIGAFGDNAILAVILGQLTFQAQAGTITEN
                     QLGATNALYTSLLFIPYVLLAPLAGFVNDRHAKTRWLLGGNGLKVAGTLVAMLSVWHG
                     AFWQGLGYLIVGIGACFYSPAKYGILPEIVPHGHLVKANGTIEFLTLVAILTGYIGGA
                     MMIDVLPVATCYLILLALFSLALGLNACMAATPDHPELRIGRSMDEFFSNFGRLLQNR
                     RLARVLCGTGLFWTCGAVIKMNFQPWGLEVLKLENNRQIALLGLWLTLGIMGGAMLAG
                     QLHRVGDLRCTRRYGWLLAASIALMGCVEFFRDTGLIRGRLSVIGILLAVGGIAGLFL
                     IPLNAALQSESDQSKLGKTIATQNFIDNLGMLGAGAIVFTGNQAGLTASGLFLLLSAL
                     VALVAVALKVPPGRGAHETPVPSAPTLAANHNGVRANPIRGGS"
     gene            7034..7978
                     /locus_tag="PKK20_01210"
     CDS             7034..7978
                     /locus_tag="PKK20_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNU98541.1"
                     /translation="MKTVPLGKSALQSSRLAYGGWRLASPSETVQVTPEREERGKAAV
                     IAAFEAGFTLFDLADIYSDGASERIFGSALKDVAGMRNKVLIATKCGIRKAGQPEPNA
                     PHRYDFSKEHILRSCDESLERMGIDTIDLYQLHRPDFLCDPAEVASAFTQLKEQGKVR
                     EFGVSNFRPSQVTVLQSACPMPLVANQIEISLAQIAPFLDGTLDHCLLTGMTPIAWSP
                     LAGGRLGTGARVSMQDNDHAHRQKVHDAMDLLARERGVDRSQIAIAWLLRHPSGIVPI
                     VGTTNPERIALAANADNIELTREEWYRLFEAAYGQRLP"
     gene            8220..8810
                     /gene="thpR"
                     /locus_tag="PKK20_01215"
     CDS             8220..8810
                     /gene="thpR"
                     /locus_tag="PKK20_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNU98542.1"
                     /translation="MGHALRLFAALTLPEALVDRLISVQRILDDRWPARSVRWVRREQ
                     LHLTLRFYGNVPPEQAPALTEALRAACAETRPLSLALAGLGAFPNPRRPRVLWAGVTG
                     DLESLERLEQRIQSATAAFGDHRETRPFQPHLTLGRIADDRERQTASPANARWDRIEN
                     DLFGSWTAAAVHLIRSELNPAGARYTTLTVAPFLRG"
     gene            8847..11099
                     /locus_tag="PKK20_01220"
     CDS             8847..11099
                     /locus_tag="PKK20_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98543.1"
                     /translation="MVQTRFLSMVLTGCLLGGIPCTGGPADTARVAVDLSWDAASARE
                     KEKPFRDLFTDTWVATDALGRAVPTHTEVGPPRADRTVGIFYFLWHGQHVQGGPYDIT
                     RILERDAGAMLKPDSSLWGPLHAPHHWGESIFGYYLTNDEGVLRKHAQMLADTGIDVV
                     IFDVTNQITYRDWYRALLRVWSEMRALGNRTPQVAFLTPFWDPPKVVRELWRDLYAPG
                     LERELWFHWDGKPLILADPDLIFDQGENAKQNTAAPLDPGHTLAQSFRVERPIRAVSA
                     RFPTWGATNSAVTLTLRRGGPLGDSVAGRRFERVADNAWLVLPLPSPQPPGFYWIEAS
                     EPEGRIGWWSHTDDTFAAGEAFADGEPVPGDRTLRFVHADGEAARIREFFTFRKPQPD
                     YFQGPTRPDMWSWLEVSPQHVFVNARGEKEQMSVGVAQNAVGNRLATMSEPGARGRSY
                     HQGAADTRRDAVRWGFNVTEQWERALKEDPRFVFITGWNEWIAGRFNEFNGVRLPVMF
                     VDQFDQEHSRDIEPMRGGHGDDYYYQMAAYVRRYKGARPLTPVRPQPILIDGEFADWV
                     GVVPEFRDTVGDPARREHRGWDPSVTYTNRTGRNDLIAAQISFDSDRAYFHVRAREPI
                     TPADDADWMLLFIDADADAATGWLGYDFVVNRSRQNPNEASLERNVEGRYAWSPVGTA
                     PFRAAGADLELAVSWSALGMRKPPARLDFKWADGIRQSGDWSDLTLNGDVAPNDRFNY
                     RALLTPSESP"
     gene            11438..11836
                     /locus_tag="PKK20_01225"
     CDS             11438..11836
                     /locus_tag="PKK20_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNU98544.1"
                     /translation="METVRRFFERDQYAKLAGIELLALEPGHATARMPLRPTHWNGLD
                     MTHGGAIFTLADFVFAAASNSHGTVAVAINASISFLKASSTGALTAEARELARNYKTG
                     TYLVEVKNDQGELIAIFQGMVYRKQDKLPA"
     gene            complement(11881..12342)
                     /locus_tag="PKK20_01230"
     CDS             complement(11881..12342)
                     /locus_tag="PKK20_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98545.1"
                     /translation="MKLVRTDGNLCVFRLGQRERDLFTQVLRRHPLVPTGYHRYTRAG
                     ATADDPANQALLDEAMAGHRAEHRERVEGLLNDPSRLGSDEKGWRLTLSREDIEWLLQ
                     VLNDVRVGSWLKAGCPDPDGKTPPKITAANATFFALIEAAGHFETALLDVL"
     gene            complement(12339..12650)
                     /gene="clpS"
                     /locus_tag="PKK20_01235"
     CDS             complement(12339..12650)
                     /gene="clpS"
                     /locus_tag="PKK20_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNU98546.1"
                     /translation="MASIGTPVVIPDLEQDQAGKSKGALDPGYLVICWNDPVNLMTYV
                     THVFQMVFGWPRQKAEQHMLQVHRQGKSVLARETLERAEFYVHQLHKYSLHATVERDK
                     G"
     gene            12868..13863
                     /locus_tag="PKK20_01240"
     CDS             12868..13863
                     /locus_tag="PKK20_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HNU98547.1"
                     /translation="MKAAYITQTGPPESIRYGDLPEPQPAGNRALVRVRAVAVNPIDT
                     YIRSGLVQMDLPTPFIVGCDLAGVVEAVGPDVNGLEPGDRVWGSNQGLLGRQGTFAEF
                     VSVDENWLYPTPPTVSDEQAAAIAMVGITAHLGLVRDARLQRGETVLVNGASGGVGST
                     VVQIAKLLGARVIATVGGHERIEAALKLGADHVIDYKTVEVDSAVKKIAPGGVNVWWE
                     TAREPNFDRVVTALASRGRMILMAGREARPQFPVGPFYVRGCSLHGFVMFSAHPDEQR
                     ECADDLNDWMVAGRLNPRIDRVMPLAETGVAHRLQEQNTIGRAGTLAGKIVLKPD"
     gene            complement(13894..15165)
                     /locus_tag="PKK20_01245"
     CDS             complement(13894..15165)
                     /locus_tag="PKK20_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNU98548.1"
                     /translation="MLTSARQLGWISATALVVANMIGTGVFTTSGFLLKDLGSPWAVL
                     AVWAGGGFLAAIGALCYGALARRIPESGGEYLFLSKTLHPAAGYVAGWISLFVGFSAP
                     LAAVAYGFGQYAKPWFGGIDAKLNGSILLVLFAFIHAADVRRGAWLQNWIVALKLVLL
                     LGFVGLAVDRIDALPAVNLGSATVPAFAVSLVWVSFSYAGWNAAVYLGGEVRDAERTL
                     PLAMLLGTAIVTALYLALNAVFVFSTAPEKLAGQLEVGRIAAEALGGVRLANVVTAVV
                     LLALATSASSLIMAGPRVYACMGADGYLPRFLASATGPPRAAIGLQVLLALLLLWSST
                     YEMLLTFIGFTLSLSTAATVLGLVRLRVREGVAVRVVGWPWLPGLFIAAVLAIAGFAI
                     VRQPIASGLGLLTLGAGWLAWRFSARRIRHP"
     gene            complement(15212..17176)
                     /locus_tag="PKK20_01250"
     CDS             complement(15212..17176)
                     /locus_tag="PKK20_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU98549.1"
                     /translation="MNRRSFFTLAGGMAGYTVAVRVNGLGAEPESRERNTRQFESIAP
                     SQPPGAIAPGDSHLTPVGLEADVLVAGGGMAGVCAALAAARHGATVILVQDRSRLGGN
                     SSSEVKMHIVGANVHGGRPGWREGGLIEELRLEDAVRNPQRSFELWDLLLYDKIKSEP
                     NITLLLDTVVFAAETRDGLIRRVLARCDKTEHLYRIEARLFLDCTGDCRLGLECGAEM
                     RMGREAKSEFDESLAYETADGQTQGSSILFTARDYGRPMPFVAPRWARKVTKEMLKFR
                     GVSSWDYGYWWIEWGGQLHSIRDNERIRFELLSIVLGVWDYIKNSGNHPTSANWALDW
                     VGMVPGKRESRRLVGDHVLTQHDLEGKNGEPDDAVAIGGWPMDDHPPSGFDAFDQPPS
                     RSVKLADVYSIPLRCLYSKTIGNLFMAGRNISASHVAFSSTRVMATCAAMGQAAGTAA
                     ALCVRYGTNPRQLCADRSRLLELQQILLRDDQTLKDRRNADPNDLARQATVTASESHD
                     LSRPEHVINGWVRDMPTEWRNRWGAKLGPDGAWLELAWAKPQRIRELQIVFDTGFHRE
                     LTLSASALVSRQIVRGPQPETVRDYVVLCAPPDGPDGAPLREVLKVTGNYQRLNRHTL
                     EPFETKRLRLHFTATNGDPIVRVYEVRVYG"
     gene            17390..>17901
                     /locus_tag="PKK20_01255"
     CDS             17390..>17901
                     /locus_tag="PKK20_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNU98550.1"
                     /translation="MLRQGGIEHHMASANDLRKGMAIMHNGDVCVVLDMQHRTPGNLR
                     AFVQATLRSVRTGKSSDIRFSSTERVDVVPMVTRKMEFSYKDGTDFVFTDPENYETVT
                     LVPEMVGDAKNYLVENGLVTVTFVDDKAVSVEIPPSVILTVTDAPEGVRGDSANNVQK
                     AVTIETGITV"
BASE COUNT         3262 a         5763 c         5845 g         3031 t
ORIGIN      
        1 atttccaacc tcaacaacag caacccttca ggaatttaaa tgaaataacc gtgggccccg
       61 tcgccgacac acgccgtctt cacttgacat gacggggggc gagccgtctc cactccgacc
      121 tctttgactt catgaattgg cgctgactca cagcctgtgc ggcatgagac ttctttcgaa
      181 aagaaacgaa gttgacagag agtggcacac cccgatggcg cacggggtac tgagacttcg
      241 caagatgaat gctacgccgg ctggaaatgc agcaccgcaa tggggaacgc cctcgtcgcc
      301 gcgcgggggg agaatcggga cgctcagccg ttgccgaaca cgctccggta gacagcccgg
      361 atgttctggc ggtagcgccc gacggcggca aggaaatcgc cggcattggc gaagccgcat
      421 cggacagcga ctcgatactg tggggccggg tcgtcgggga gttcggtttc gcccgcgtaa
      481 ctccagcgcc gcaagattcc ctcgatgcgc cgcaagcggc ggtaattctc gatgagctgc
      541 tgggcggcgc cgggatcgag cgccttctga tcgcgggcct ggcggagggc ttcgagggtg
      601 ttgggttgct gccagccacg cgccagacag agcatctggg cgatgaactc cgcatcgacc
      661 aggcctcccg cgccggtctt gatcgccaga tgctctttgc ccttgggcgt tctctcctgt
      721 tcgatcctca ggcgcatccg ggcaatctcc cctttccaat cggggtcgcg cgcgggcgcg
      781 gcggcggtgt ccgggcgact gaaatcggcc agtgtcgcca ccatctggcg gtagcgcgcg
      841 ccgacctcgc gcgagccggc gacgaggcgg gacctggaga tggcctggat ttcccagagg
      901 cgggcgcgtt gccggtagta atccccgtac gcatcgagcg tgttgacgag gaggcccttt
      961 tcgccatcgg gccgcaggcg ggcgtccacc tcgaaggcca cgcccagctc ggtcgggctg
     1021 cccagcaaat ccatcacctc gacagcgacg cgctgcaggc cgggcaggtc tcgggtcttc
     1081 ggatcggcga caaacacgac atcgaggtct gagccatagt tcagctccgc tccgcccagc
     1141 ttgcccaggc cgacgatcgc gatgcattcg gggcgccgac ggtgtttgcg cagcgcgact
     1201 tcgagtgcgt agtccagaca ggcatcggcc agcgccgaca actcgacgag gttttgctcg
     1261 aaatccgcca gcccgagaat atcgcgcaaa ccgatgcgca tcagttcggt ctggtggtag
     1321 cgcctgagcc agagtttctg gtcggggtcg tccagtccgt ggcgcaggtc tttgagaatc
     1381 cgggcggcgt tcttggcgcg gcgcaggtag ccgctcagtt cgagttcgtc gaccagatcg
     1441 ggggtgcgga tggcggtctc ggccaggaac tcggagcgat cgaacaggag gatgatgagc
     1501 tcgaacaggg agggattcgt agcccacgtc tcgaacagca gggcccgccc gccgtaggcg
     1561 gcgacgaacg tgtccagacg cgccaggacg cggtcgggat ccgagaggcg tttcgagtgc
     1621 gggtcgccat cggggaagcg ggcggcgtct tgcgaatcgg cgacgccgcg cgccgggcag
     1681 agattcaaga actgagcgag cagttggcgt ccgaacttct cggtccgggc cgacagatgc
     1741 ccgaagccgg gtccctggac gagggcgcgg accaggcgga gggccttgtc gggatcgcgg
     1801 aaggagaggt cgtggaggag gctcttccac tggtcttcct gcgcggtcaa gtcggggaac
     1861 tggagggatg gctgaggcgc gtcggtgtgg aggaggcggt cgtagatggc gcggacgcgt
     1921 tcccgatgat cgagcaaagc ggtttcgaag gcggccaggg cgtcatagcc cataagccga
     1981 gcgagccgtt cccgtttctc ccgttcggtc gggatcgtgt gggtctgcag gttgttgtcc
     2041 atctgcagcc ggtgttcgat gtcacgcagg aacagataag ccgctccaag cgcctgcgcg
     2101 tcctcggtgg gaagcagttg gtactcggcc agcttggcca gcgccggcag tgtctgcgaa
     2161 tgcgccagaa acggatgtcg tccggcgtgc atcatctgca gcgtctgagc cacgaactcg
     2221 atctctcgaa tgccgccccg cccgagcttg acgttgcgat cgatctcgcc cgcgcggacg
     2281 atttcgtttt cgatgcgctg tttcatgtcc gccacatcgt ccagcgcctg acggctgagc
     2341 gaccgcgggt agcgaaacgg ctgaatcatc tcgatgaatt cgttcgccaa cgcggcgtct
     2401 cccgccacac ggcgcgcctt gatcagcatc atgcgctccc aggtctggcc ccactgccag
     2461 taatagttct cgcagccggc cagggagcgc gccaacggcc cccggtcacc ctccggccgc
     2521 acccgcaggt cgatgcgata gagcgttccc tccggcgtca tgcgggcgac ctcggccacg
     2581 atcgcctcgg tcaggcgctg gaaatagagg tggttggcaa ctcccttgcc cgtctcgccg
     2641 ggggccggcg actcgcgaaa gccgaatccc tcgtcgctgt agaggaacat cagatcgaca
     2701 tccgagctgt aattgagttc ctggccgccg agcttcccca ggccaagcac acagaaggtc
     2761 gtcggacgcc aaccgccccc ggggtccgtg tgccacggct taccgaggcg cccctcgatc
     2821 tggcgccggc acaactcgca caccgtctcc agacagaggt cggctagatc cgaaagctcg
     2881 cgtgtgatca ccgcggcctt tcctggcccc gacagatcgc gaatggcaat ccgcagtgtc
     2941 tcccgctgtt tgaagagcct gagctgggca aaagccgccg agtcgtcggc ccgcttgatc
     3001 agcgggccga tggctctctg gaggtctgcc ctcagacgcg gcatggagcg gggggtgctc
     3061 agagtcttga catcgagcag ggcaggaacc caatccggat gcgcgatgag cgtctcgatc
     3121 gagacctgcg aaccgctcag cagggccgcc agcattcgcg ccgcctccgc cttggccatt
     3181 tgaagcgcgg gtttcgcctc cgtcgcccag agcaatccca gggagtgctc cgcccgcctc
     3241 gggtcggcgc atctcgcaat cgcatccgcc caggtccggc ttcgcatgcc cggattagag
     3301 aggagggcgt gcgcggtttc aagcctggcg atgggggggc aggtcttgca cccttctgga
     3361 caactccatg tgactgacct tgccagtgcc cagtttcgga atctggcgca ggaaccggac
     3421 ctcccgaggc acgcacaggt tggtgaagcc cttggcgcgg atcaccgcgc gaatctcgtc
     3481 cagcgtgagc cgggcttcgt tggtgacggc gatgatcgtt tcgcctttct ccgcgtccgg
     3541 gcgtgcaacg atggcgactt cgaaccggac gccgtactgg gggaatgcgc cggccagggc
     3601 gtcttcgatg gccgtcaggc tgaccatttc gccactgacc ttggcgaagc gcttcaggcg
     3661 tccgaggatg tggacgaacc cgtcggcatc cacgttgacg acgtcgcccg tatcgtacca
     3721 gccgcccagg ctcttgaact tcgcgttcgc gtcggggttg agatagccgc gcatgacgtt
     3781 ggggccgcgc accagcagac gcccgccatc gccgacgccc tcgacggatt cgagtttgta
     3841 ttcgaggccg ggcaggaagc gtccggccga tccgtgccgg gaggcctggg gcaggttgac
     3901 gctgatgacc gggctgcatt cggtggcgcc ataaccctcg agaatgcgca cgccgaaccg
     3961 ctgcatccag aggcgcgctg tctcgtcctg gaccttctcg gccccggcga agagatagcg
     4021 cacggcgcga tagtcgtacg gatgggcgcg gcgtgcgtag ccattgagga atgtgttcgt
     4081 ggcgaggaat acggtgcaat agcggtcgta gaggacattg ggtatgacgc ggtaatgcag
     4141 cggcgagggg tacatgaaca cgtagaggcc gcgcacgagg ccgagcagag tcccgacggt
     4201 caatccgaag ctgtggaagc aaggcagggc gttgaagaca cggtcccgat cttcgaggtc
     4261 gatcaccgtc agcatttgtc ggatattcgc caggagattg ccatgggtca gttcaacccc
     4321 tttgggagct ccctcggatc cgctcgtgaa cagaatcgcc gcggggcggt ccgaggcgct
     4381 gaatccgccc gcggtgggcc gatcggaccc gcgtgcgggc cggagtgtcg acgccggcgc
     4441 caggatcacg cgcgccagcc ccagcagccg ctctccgctt gtgacgctct tccgcacgtc
     4501 ctcgaggtag atcaccttta gaccggcggc cacaaacggg tccgcgctga gcttcgcgcc
     4561 cgccagaaag gcgcgcgcag tcaccagcgc ctcgagtccg gccagccgag cgcaggccag
     4621 catgacgggc accccggtcg agaaattcag aatcgcgggc actctgccca gagaccaaag
     4681 cccgagcaga agggtgggca tggcgttggc gtttggcagc agcaccccca ccctgtcggt
     4741 gttgggcggg agttgggcgt ggagcttccg gcccacgagg tgcgcgccgg tcagaagacg
     4801 ccggtacgtc agagactggg tggtcgcatc ctgaagaacg gtcttgcccg gctgtgcccg
     4861 ggcagcctcg aggatggcgt cgaggacatg cttcggtcca aacgccatct cggtctcgaa
     4921 ctgctgcagc agcatctggt cgcggagcca gttgctgaga aacgcgcgcg cctcggcggt
     4981 gctctgatac tggcgctgag ggggtgcgag cggcgggctg aaatggacgg tgatcgtggg
     5041 ccaccagcgc cgccacccgg gctgcctgga ccagcgcagc cggttggctc cccggatgta
     5101 gcaggcgatg agtcgggcgc gggtcttgtg catcaggaaa ccggtccctt catagacttt
     5161 catgagcgaa ccggtgcggg tgatttggcc ctcggcaaag agcaccaggc gccctccccg
     5221 ttcgaggcac tcggccatgc gcttggccgc atagggcgag ttcgtatcga cgggaaacgt
     5281 ccggcggttg agcataatcc tgcgatggac ccaactgtac tgggcggcga cgttcgagac
     5341 gacgcacttc caatccaggt ccaacagcac gtagagatag agccaatcga gccaggagac
     5401 gtggttcggt atgaggagca cggggcccgg tgtcttgagc acatccgtgt tgtaggcgcg
     5461 aaaaccgaaa cagagcctaa gcaggaaacg aaggatcgtg cgcatggagt caaggcatgg
     5521 gcagagggcg tcatgaaccg ccgcggatcg gattcgcccg cacgccgttg tgattcgccg
     5581 cgagagtggg cgcggacggc accggcgtct catgggcccc gcgtccgggc gggaccttga
     5641 gcgcgaccgc caccagcgcc accagcgcgc tgaggaggag gaaaagccct gatgcggtga
     5701 ggcctgcctg gttgcccgta aacacgatcg ctcctgcccc gagcatgccg aggttatcga
     5761 tgaagttctg ggtggcgatc gtcttgccca acttgctctg atcggactcg gattgcagcg
     5821 cggcgttgag cgggatgaga aacagaccgg caatgccccc gaccgccagc agaatgccga
     5881 tcaccgacaa ccgcccgcga atgagcccgg tgtcccggaa gaactcgacg cagcccatga
     5941 gggcgatact ggcggcgagg agccatccgt accgccgtgt gcagcgcaaa tcgccgacgc
     6001 gatgcagttg gccggcgagc atcgcgccgc ccatgatgcc gagggtgagc cagaggccaa
     6061 ggagtgcgat ctgccgattg ttctcgagtt tgagaacttc gaggccccag ggctggaagt
     6121 tcatcttgat caccgcgccg catgtccaga aaaggcccgt gccgcagaga acccgggcga
     6181 gccggcggtt ctggaggaga cgaccgaagt tcgagaagaa ctcgtccatg ctgcgcccga
     6241 tcctcagctc ggggtgatcg ggcgtggccg ccatgcaggc atttaggccg agagccaggc
     6301 tgaagagggc aagcaggatg aggtagcacg ttgccacggg caggacatcg atcatcatgg
     6361 cgcccccgat atagccggtc aggatcgcga cgagggtgag gaactcgatt gtgccgttgg
     6421 ccttgaccag gtgtccgtgg ggaacgatct cgggcagaat gccgtacttg gcgggggagt
     6481 agaagcaggc gccgatgccc acgatgaggt agccgagccc ctgccagaac gcgccgtgcc
     6541 agacactgag catggccacc agggtgcccg ccaccttgag gccattgccg ccgagcagcc
     6601 agcgcgtctt ggcgtgccgg tcgttgacga agccggccag gggcgccagg agaacatatg
     6661 gtatgaagag cagactggta tagagggcgt tggtggcgcc gagttggttc tcggtgatcg
     6721 tgccggcctg cgcttgaaag gtgagttggc cgaggatgac agccagaatc gcgttgtcgc
     6781 caaacgcgcc gatcaactgg ctggccagca gcaatcgata attccgcgcg cggttcatga
     6841 gaggcgcgcg tcgtggcgga caaccggcac gccgggcggt gtcatcggag cctgatcacg
     6901 cggagccaac tgggtcggga aggaccacac ggagactgtt tagatgagga gagtgtgggt
     6961 gtcaacaggc ggccgaggcc gaaaacgctt cctatgggcg ggtcgagctc gctacactgc
     7021 atcgcataca tctatgaaga ctgttcctct tggaaagagc gccctgcaat cttcgcggct
     7081 ggcctacggc ggctggcgcc tggcaagccc gagcgagacc gtccaggtca cccccgagcg
     7141 ggaggagcgc ggcaaggcgg cggtgatcgc cgcattcgag gccggtttca ccctgttcga
     7201 tctggccgac atttacagcg acggcgcatc cgaacgcatc ttcggttcgg cgctcaagga
     7261 cgtggccggt atgcggaaca aggtgttgat cgccaccaag tgcggcattc gcaaggcggg
     7321 tcagcccgag cccaatgcgc cgcatcggta cgatttttcg aaggagcaca tcctgcgttc
     7381 gtgcgacgaa tcgctcgaac gtatgggaat cgacacaatc gatctgtatc agctccaccg
     7441 tcccgacttc ctgtgcgacc ccgcggaggt cgcctcggcc ttcactcagt tgaaggagca
     7501 aggcaaggtc cgcgagttcg gcgtgagcaa tttccgccct tcgcaggtca cggtcctgca
     7561 atcggcctgc cccatgccgc tggtcgccaa ccagatcgaa atcagcctcg cccagatcgc
     7621 cccgttcctc gatggcacgc tcgatcactg cctcctgaca ggcatgacgc ccatcgcctg
     7681 gagcccgctg gcgggcggac gcctgggcac gggagcccgc gtgagcatgc aggataacga
     7741 ccacgcgcac aggcagaagg tgcatgacgc catggatctg ctggcacgcg aacggggcgt
     7801 cgatcgcagc cagatcgcca tcgcctggct gctccggcat ccctccggga ttgtcccgat
     7861 cgtcggcacg acgaatccgg agcggatcgc gctggcggcc aatgcggaca acatcgaact
     7921 gacccgcgag gaatggtatc ggctgttcga ggccgcctat ggacagaggc tgccgtaacc
     7981 cggaaacctc atgccgggac gaggacgtgg atttccgggc tgtggccgtg agtcccgttg
     8041 cggttctcca cgaagcctgt gcaccgatgc cccgaaatat ggccagcccg cgatgaatcg
     8101 cggacgagca agctccgcga gaccctgcct tgagacaatc actgtcattt attagggcct
     8161 ctcccgcagc ggcgagatcg gccattcgag aaggatcgag cctccgacac agaccggcca
     8221 tgggacatgc gctgcgcctg ttcgccgcgc tgacgctccc cgaagcgctt gttgaccgcc
     8281 tgatttctgt tcagcgcatt ctcgatgacc gctggcccgc ccgttcggtc cgctgggtgc
     8341 gacgcgaaca gctccatctg accctgcgtt tttacggcaa cgtgccgccc gaacaggccc
     8401 ctgccctgac tgaggccctg cgtgccgcat gcgccgaaac ccgtccgctg tcgctcgcgc
     8461 tcgccggctt gggcgctttt cccaatccgc gtcggcctcg agtcctgtgg gccggcgtca
     8521 cgggcgatct cgaatcgctg gagcgcctcg aacagcgaat tcaatccgcc acagcagcgt
     8581 ttggcgacca ccgggaaacc cgcccatttc aaccgcacct gactctaggg cgcattgcgg
     8641 acgaccggga acggcaaact gcatcgccgg cgaacgcgcg ctgggaccgg atcgagaacg
     8701 acctctttgg ttcctggacc gcggcggccg ttcatctcat ccgcagcgaa ctcaatcctg
     8761 cgggcgctcg ctacacgacc ctgaccgtgg cgccgtttct tcgaggctag ccttgagcgg
     8821 gccacgccgg cacctacctt acgcgcatgg tccagacacg atttctatcg atggtactga
     8881 cgggctgcct gttgggcggc attccctgca caggcggccc ggctgacacc gcgcgcgtcg
     8941 cggtggactt gtcgtgggat gctgcttccg cgcgcgagaa ggagaagcct tttcgcgatc
     9001 tgttcaccga cacctgggtg gccactgatg ccctgggacg cgccgtgccc acccataccg
     9061 aggtcggccc gccgcgggcc gatcgcacgg tgggcatctt ctactttctc tggcacggcc
     9121 agcacgtcca gggcgggcca tacgatatca cgcgcatcct cgaacgggat gccggcgcga
     9181 tgctcaagcc ggacagctcg ctctggggcc cgctgcacgc cccgcatcat tggggggaat
     9241 cgatcttcgg ctattacctg accaacgacg aaggtgtcct gcgcaaacac gcccaaatgc
     9301 tcgccgatac cgggatcgat gtcgtgattt tcgacgtcac caaccagatc acctaccgcg
     9361 attggtaccg cgcactgctg cgggtgtggt cggagatgcg cgccctcggc aaccgcaccc
     9421 cgcaggtcgc cttcctgacc ccgttttggg atccgcctaa agtggtgcgc gaactctggc
     9481 gcgatcttta cgctccggga ctcgaacgcg aactctggtt ccactgggac ggcaagccgc
     9541 tcatcctggc ggatccagac ctgattttcg accagggcga gaacgccaag cagaacacgg
     9601 cggcgccgct cgatcccggc cacacgcttg cgcaatcgtt ccgggtcgag cgccccatcc
     9661 gggccgtgag cgcgcgtttc cccacgtggg gcgccaccaa cagcgcggtg acgctgacgc
     9721 tgcggcgcgg tggaccgctg ggcgatagcg ttgccggccg ccgattcgaa cgcgtggcgg
     9781 acaatgcttg gctggtgctc ccactcccca gcccgcagcc gccgggattc tactggatcg
     9841 aggcctccga acccgaaggg cgcatcggat ggtggagcca caccgacgat acctttgcgg
     9901 ccggcgaggc gttcgcggac ggagaaccgg tgcccggaga ccgcacgctc cgtttcgtgc
     9961 acgccgatgg cgaggcggcc cgcatccgcg agttcttcac ctttcgcaag ccgcaaccgg
    10021 actatttcca ggggccgacc cggccggaca tgtggagttg gctcgaggtc tccccgcagc
    10081 acgtgttcgt caacgcccgc ggcgagaagg agcagatgtc tgtcggcgtc gctcagaatg
    10141 ccgtcggcaa tcggctggcc accatgagcg agccgggggc gcgcgggcgg agctaccatc
    10201 agggcgctgc cgacacccgg cgggacgcgg tgcgctgggg cttcaacgtg accgagcagt
    10261 gggaacgcgc cctgaaggag gacccgcgat tcgtcttcat cacgggctgg aacgagtgga
    10321 ttgcggggcg cttcaatgaa ttcaacggtg tccggctgcc ggtcatgttc gtcgatcagt
    10381 tcgatcagga acacagtcgc gatatcgagc cgatgcgggg tggtcacgga gacgattact
    10441 actaccagat ggcggcgtac gtccgccgtt acaagggcgc ccgccccctc acgccggtcc
    10501 gcccacagcc gattctcatc gacggcgagt tcgccgattg ggtcggcgtg gtgcccgagt
    10561 tccgggacac cgtcggcgat cccgcgcggc gggaacaccg cggctgggat ccgagcgtca
    10621 cctacaccaa ccgcaccgga cgcaatgacc tgatcgccgc ccagatcagc ttcgattccg
    10681 accgcgccta ttttcacgtc cgcgcccgcg agccgatcac gcctgcggac gacgccgatt
    10741 ggatgctgct gttcattgat gcggacgccg acgcggcgac cggctggctg gggtatgact
    10801 tcgtcgtgaa ccgttcgcgg cagaatccaa acgaggcctc actcgaacgc aatgtcgagg
    10861 gccgctacgc atggagcccg gtcggcacgg cgccattccg cgcggcgggc gccgacctcg
    10921 agttggctgt ttcgtggtcg gcgctcggca tgcgcaagcc gccggcccgg ctcgatttca
    10981 agtgggcgga cggcatccgc cagagcggcg actggagcga tttgacgctc aatggagacg
    11041 ttgcgccaaa cgaccgattc aactaccgcg cgctgttgac tccatccgag tccccatgac
    11101 ctccgtgccg cgccggcccg ggctgatgac aggctccgcg ctcaacatga tccgggtcag
    11161 tcggcaaggc acggaaacga atctgcctgt gtccgggtct tccggcaatg catgtggagt
    11221 atgtcgcagg gccaattcat gaagtggagc ggcgtcgcct acaaacgccg tcttcacttg
    11281 acgtggcggg ggacgagccg tccccacccc gaccttttcg acttcatgca tgggcgctga
    11341 gtgtgtcggg aatcatcttc agactcgact cgaaggcagg atcggactac agacaggtgt
    11401 gcgccgcgcg ctcgaggcgc cggcattgcg accgaccatg gaaacagtca gacgattctt
    11461 cgaacgggac cagtacgcaa aactcgccgg gatcgaactg ctcgcgctcg agcccgggca
    11521 cgccacggcc cggatgcctc tgcgccccac gcattggaac gggctcgata tgacgcacgg
    11581 cggcgcgatt ttcacgctgg ccgactttgt ttttgcggcg gcgtccaatt cccatggcac
    11641 cgtggcggtc gcgatcaacg ccagcatctc gttcctcaag gcctcgagca cgggcgctct
    11701 gactgccgag gcgcgcgaac tggcccggaa ctacaagacc ggcacctacc tggtcgaggt
    11761 caagaacgac cagggcgaac tcatcgccat cttccaaggc atggtttacc ggaaacagga
    11821 caagctccct gcctgaccgg gcacctggcc gccggtggca ggccaggcgc ctcccgccag
    11881 tcacagcaca tcgagaagtg cggtctcgaa gtgcccagcc gcttcgatca gggcgaaaaa
    11941 cgtggcattg gccgcggtga tcttcggagg ggtcttccca tccggatccg ggcagccggc
    12001 cttgagccag cttccgaccc gcacgtcatt gagcacctgc agcagccatt cgatgtcctc
    12061 acggctaagg gtcaaccgcc agcccttttc atcggaaccc aggcgtgacg gatcgttcag
    12121 cagaccttcc acacgctctc ggtgctcggc ccggtgcccc gccatggcct cgtccaacag
    12181 cgcctggttg gccggatcgt ccgcggtcgc ccctgcgcgc gtgtagcgat ggtagccggt
    12241 gggaaccagc gggtgacggc gcaggacttg ggtgaacaga tccctctcgc gctggcccaa
    12301 acggaagacg cagaggttgc cgtctgtgcg taccagtttc atcccttgtc gcgttcgacg
    12361 gtggcgtgga ggctgtactt gtggagttga tgcacgtaga actcggcgcg ttcaagggtt
    12421 tcgcgggcta acacgctctt cccttggcgg tgcacctgca gcatgtgctg ctccgccttc
    12481 tggcgcggcc agccgaacac catctggaac acgtgtgtca cgtacgtcat caggttcacc
    12541 ggatcgttcc agcaaatcac caggtagccc ggatcaagcg cgcctttcga cttgcccgct
    12601 tgatcctgct cgagatccgg tatcaccacc ggtgttccga tcgatgccat gtccgaacat
    12661 agccagggat ggcctctgtt tcaatacgcc ggattgctcc tccggtgatc gggcccggtg
    12721 ccctcaccgg gcgacagaac accgacaacg atgagatgca gcggagcggc cgacacggac
    12781 gagcggcggg ttgcaaattc gggcaatgca tctagaattg cgcttgtttg gagtctgtag
    12841 cagcctgctc aactacatcg gtaaaccatg aaagccgcct acatcactca gaccggtccg
    12901 cccgaatcga tccgatacgg tgacttgccc gagccgcaac ccgcgggtaa tcgcgccctg
    12961 gtgcgcgttc gcgccgtggc ggtcaacccg atcgacacct acatacgcag cgggttggtc
    13021 cagatggatt tgcctacgcc gttcattgtc ggatgcgatt tggcgggcgt ggtcgaagcg
    13081 gtcggtccgg acgtgaacgg cctcgagccg ggggaccgcg tctgggggag caaccagggg
    13141 cttctcggac ggcagggcac tttcgccgag tttgtatcgg tcgatgagaa ctggctttat
    13201 cccacgcccc ccacggtctc cgatgaacag gcggcggcca tcgccatggt cggcatcacc
    13261 gcgcatctcg gtctggtgcg ggacgccaga ctccagcgcg gcgagacggt cctggtcaac
    13321 ggcgcaagtg gcggcgtcgg ctcgactgtc gtccagatcg ccaaactgct cggggcccgc
    13381 gtcattgcca ctgtgggcgg acacgagcgg atcgaggccg ctctcaagct gggggccgac
    13441 cacgtcatcg actacaaaac ggtcgaagtc gactccgcgg tgaagaagat cgccccgggt
    13501 ggcgtgaatg tctggtggga gacggcccgc gaaccgaact tcgatcgggt ggtcaccgcg
    13561 ctcgcctcgc gggggcgaat gattctgatg gccgggcgcg aggcccgccc tcagtttcct
    13621 gtcggcccct tttatgtccg cggctgctcg ctgcacggct tcgtgatgtt cagcgcccac
    13681 cccgacgaac agcgcgagtg cgcggacgat ctcaacgatt ggatggttgc cgggcggctg
    13741 aacccgcgga tcgaccgcgt gatgccgctc gccgagaccg gtgtcgctca ccgactccag
    13801 gaacagaaca ccatcggcag agccggcacg ttggcgggca agatcgtcct taagccggat
    13861 tgaacccatc tccatctctg ggtctgacga ccgtcacggg tgtctgatgc gccgcgccga
    13921 gaaccgccaa gcgagccaac cggcccccag agtcaacagg cccagcccac tggcgatcgg
    13981 ctgacggaca atggcgaacc cggcgatcgc cagcacggcg gcgatgaaca gacccggcag
    14041 ccacggccag ccgaccactc gcacggcgac tccctcccgc accctgagcc ggacgagccc
    14101 gaggacggtt gcggccgtgc tcagactcag agtgaacccg atgaaggtca gcaacatctc
    14161 gtaggtcgaa ctccacaaaa gcagcaaggc cagcaggact tgcagcccaa tggccgcgcg
    14221 cggcggaccc gtcgccgacg ccaggaaccg cgggagatag ccgtccgcgc ccatgcaggc
    14281 gtacacccgc ggcccggcca tgatcagcga ggacgcggat gtggcgagag cgagcaagac
    14341 gaccgcggtc accacgttgg ccaggcgcac cccacccagc gcctcggcag cgatgcgccc
    14401 gacctcgagt tgccccgcca gcttctcggg agcggtcgag aagacgaaca ccgcgttgag
    14461 ggcgaggtag agcgccgtga caatggccgt gccgaggagc atggccagcg gcagggtacg
    14521 ctcagcatcc cgcacttcgc cccccaggta gaccgcagcg ttccatccgg cgtagctgaa
    14581 cgacacccaa acgagcgaga cggcaaacgc cggaaccgtg gccgagccta gattcacggc
    14641 cggcaacgcg tcgatccggt cgacggcgag gcccacaaaa ccaagcagca gaaccagctt
    14701 aagcgccacg atccagttct gcagccacgc gccccggcgc acatctgccg catggatgaa
    14761 ggcgaacaga accaggagga tcgagccgtt cagtttcgcg tcgatgcccc cgaaccatgg
    14821 cttcgcgtat tggccgaagc cgtaagcgac cgcggcgagc ggggcggaga aacccacaaa
    14881 gagcgagatc cacccggcga catagccggc cgcagggtgc agcgtcttcg agaggaacag
    14941 gtattcgccg cccgactcgg ggatgcggcg ggccagggcg ccgtaacaga gcgcgccgat
    15001 ggcggccagg aaaccgccgc ccgcccagac cgccaggacg gcccacggcg atccgaggtc
    15061 tttcagcaga aaaccgctgg tggtgaagac gccggtgccg atcatgttcg ccacgaccag
    15121 cgccgtggcg ctgatccacc cgagctggcg tgccgaggtc agcatgcgtc gattcgattg
    15181 cgccgagtcg cgtccatgag cacgggccgg atcacccgta gactcgtacc tcgtacacgc
    15241 gcacaatggg atcgccattg gttgcggtga agtgcagacg caaccgtttg gtctcgaaag
    15301 gctcgagtgt gtgccgattc agacgctggt aattgcccgt gaccttgagg acttcgcgca
    15361 gcggagcgcc gtcgggaccg tctggaggcg cgcacaagac cacgtaatcc cgcacggtct
    15421 ccggctgcgg gccgcgcacg atctgtcggc tgacaagggc cgatgcggac agcgtcagct
    15481 cgcgatggaa gccggtgtcg aagacgatct ggagttcgcg gattcgctgg ggcttggccc
    15541 acgccaactc gagccaggcc ccatcgggcc cgagcttggc gccccagcga ttgcgccact
    15601 cggtgggcat gtcgcgcacc cagccgttga tcacatgctc gggcctggac agatcgtggc
    15661 tttccgacgc cgtgaccgtt gcctggcgcg ccaggtcgtt tggatcggca ttgcgccggt
    15721 ccttcaacgt ctggtcgtcc cgcagaagaa tctgctggag ttcgagcagg cgggacctgt
    15781 cggcgcagag ctgtcgcgga ttcgtgccat accgcacgca gagggcggcg gcggtcccgg
    15841 cggcctggcc catcgccgcg catgtggcca tgacgcgcgt cgagctgaag gcgacatgac
    15901 tggcgctgat gttgcgaccg gccatgaaga ggttgccgat cgtcttgctg taaaggcaac
    15961 gcagcgggat gctgtaaacg tccgccagtt tgaccgatct cgagggcggc tggtcaaagg
    16021 catcgaagcc gctgggcgga tggtcgtcca tcggccagcc gccgatggcc acggcgtcgt
    16081 cgggttcgcc gttctttcct tcgagatcgt gctgggtgag cacatggtcg ccgaccagcc
    16141 gccggctttc acgcttgccc ggcaccatgc cgacccagtc gagcgcccag tttgcgctgg
    16201 tcgggtgatt gccggagttc ttgatgtaat cccagacccc aagcacgatc gagagcagct
    16261 cgaaccggat tcgctcgttg tcgcggatgg aatgtaactg gccgccccac tcgatccacc
    16321 agtagccgta atcccacgag gaaacacccc ggaacttgag catctccttg gtcactttcc
    16381 gggcccatcg gggcgcaacg aagggcatcg gacgcccgta atcgcgggcg gtgaagagga
    16441 tgctgctgcc ctgggtctgg ccgtcggcgg tctcataggc caacgactcg tcaaactcgc
    16501 tcttcgcttc ccgccccatc cgcatctctg ccccgcattc gaggccgagc cggcaatccc
    16561 ccgtgcagtc gaggaatagc ctggcctcga tccgatagag atgctcggtc ttgtcgcacc
    16621 gcgccagaac gcgccggatc aggccgtcgc gcgtctcggc ggcgaagacc acggtgtcca
    16681 ggagcagcgt gatattcggt tcactcttga tcttgtcgta aagaagaagg tcccagagtt
    16741 caaaactgcg ctgcgggttc cggacggcat cttcgagccg gagttcctcg atcaggccgc
    16801 cttcccgcca accgggccgc ccgccgtgga cgttggcgcc gacgatgtgc atcttgacct
    16861 cgctgctcga attgccgccg agacgagaac ggtcctgcac caggatgacc gttgcgccgt
    16921 gacgagccgc tgccagcgcc gcacagaccc cggccatgcc gccgcctgca acaagaacgt
    16981 cggcttcgag accgacaggc gtcaggtgcg aatcgcccgg cgcgatcgcg cccggcggtt
    17041 gggacggggc aatcgactcg aactgacggg tgttgcgctc gcgcgactca ggctcggcgc
    17101 ccagcccatt gacccgcacg gctaccgtgt aaccggccat cccgccggcg agcgtgaaga
    17161 aactgcgtcg attcatgcct ggcctttcgc acaccgtgtc cgcacatcag tactcgaatc
    17221 caaagcgaat gaacaccgcg aaaaactacg tgctttcccc gacgcacggc aacgactacc
    17281 ttgagcgcgt tcatggcttc ctcatgaatc aaatggttcg atcagtcgtt ttcggcgctt
    17341 gcaatcgagg cctgccagat taggctctct gggctttgcc tgggccgccg tgctgcggca
    17401 gggcgggatc gagcatcata tggcatctgc aaacgacttg agaaagggca tggcgatcat
    17461 gcacaacggc gacgtctgcg tggttctgga catgcaacac cgcacgcctg gaaacttgcg
    17521 cgcgttcgtg caagccactc tgcgcagcgt tcgcaccggc aaatcctcgg acatccgatt
    17581 cagttccacc gagcgagtgg acgtggtgcc gatggtgacg cggaagatgg agttcagcta
    17641 caaggacggc acagacttcg tcttcaccga cccggaaaac tacgaaacgg tcactcttgt
    17701 tcccgaaatg gttggcgatg ccaaaaatta cctcgtggaa aacggcttgg taaccgtaac
    17761 cttcgtggac gacaaggcgg tttccgtcga aatcccccca agcgtgattc tcaccgtaac
    17821 tgatgcgccg gaaggcgtgc ggggcgactc ggccaacaac gtccagaagg ccgtcaccat
    17881 cgagacgggc atcaccgtgc a
//
LOCUS       DAOQRO010000016        17704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2293_length_17704_cov_6.300357, whole genome shotgun sequence.
ACCESSION   DAOQRO010000016 DAOQRO010000000
VERSION     DAOQRO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17704
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2293_length_17704_cov_6.300357"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKK20_01260"
     CDS             complement(<1..784)
                     /locus_tag="PKK20_01260"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HNU98551.1"
                     /translation="MTPPSPLPGRRPGSDSRGILLALVLGTLAPDAAGAPGIATDPDE
                     PPSFRRAEFHEALVEKQARSPSQSSIGDSRSSPLRERVMAAEQVGKEPTPLADSGLDR
                     AGIHRIGLVTLDRAARTVRFPAAVNQRAGLIEYAVVTTTGKVHESIFRTEAAPRDIHL
                     AMLLLGVKPANTNVLHDDPHTPLPGERIGIEVGWQRGRKEVRRPLADFVKIGSRVRSS
                     GSRGWLYNGSFLVNGDFAAQRGGSIVSLITDASALVNNPGPDR"
     gene            complement(781..1965)
                     /locus_tag="PKK20_01265"
     CDS             complement(781..1965)
                     /locus_tag="PKK20_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HNU98552.1"
                     /translation="MCKNGEKCRLRVLGLLLCSAFCCLAPAPARADTPAPPSAAAMDR
                     AVDRGVRFLLKSQNTNGSWGSARRTKDLNIFAPVPGAHEAFRSGVTALAVQALIETGA
                     ADRDPTARAALERGEAWLLDNLPRLRRADAVALYNVWGHAYGIQALAAMHRHASPDSD
                     RRQRIADVIRGQIDLLGRYESVDGGWGYYDFRVGAQQPAAESTSFVTATVLVAFREAK
                     RIGIEIPQKLVDRGVASIKRQQKPDFSYAYAEDLKWRPMRYINRPAGSLGRSQACNIA
                     LRWWGETNITDTVLETWLERFNDRIGWLSIGRKRPVPHESWFQVAGYFYYYGHFYAAL
                     CADELEPERARTHHARLAAILLPLQEKDGSWWDYPFYDYHQPYGTGFALIALARGRAA
                     IQ"
     gene            complement(2081..3526)
                     /locus_tag="PKK20_01270"
     CDS             complement(2081..3526)
                     /locus_tag="PKK20_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98553.1"
                     /translation="MGCKSTSPGRSTAAVVPSAATPAATTPAAPKEPASHVAGRGAIK
                     RVVQFDAVLEAVEMHPVRLEPKAWADLTVVEAVPHGARVRAGDVLVRLDTDKLREQIA
                     DLEQDAPAAAIALEMARAELASLKETTPQRLAAARRSRRVAAEDQEYFEKHGRELRIR
                     SANFNVKSAEQRLANAAEELKQLRQMYEADDLTEQTEEIILERQEFEVETARFSLDNI
                     RASTERDLETAIPRETENLQSQKRDQELALALAEETLPKALMRKELEVEKLTRDQKKA
                     RKRLDDLKTDLAAMDVRAPADGVVMYGACEAGKWTTGPVVIKKLVPGGKLAPYEIFMT
                     VVNPDRFQLKAVVPEADLGKTRIGMTGEAAPVALPNCKVPVKIEAVDRVPLVTGGFGA
                     TLSLAPSGPLELQPGMNCKVTLTDTSSANALVAPVQAVFGEGDDRHVFVRVADGKAEK
                     RAVRTGESDGKTIEIVDGLAEGDTILLSKPE"
     gene            3719..5476
                     /locus_tag="PKK20_01275"
     CDS             3719..5476
                     /locus_tag="PKK20_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98554.1"
                     /translation="MGLGAETVRMERRAIAAQSGRGPMPTRRRRVWRLAWRTAGAVGA
                     GLLLLGAVCVYGWRFGFTPLPKPSPEEAWTPLRTAQPAELTRSDYWYWVDEFNRASVV
                     NRRSDIIRGWTLFDWAQTGHLTETNRAGLAAFFGGDATLESCYRGALAAGKTHTNLWE
                     RRRVGRDLAGHTRFVNHAAWKALEEEQAGRTVGALRRLLEGWHLCANAGAAGLTRPEL
                     GTLAGAWHRLALEGPGIDPSEGLQLIEGLAQVRKALPRVDVEFLRAVHSRQEVFGRAA
                     SEGQLPPAIGRQFWAAFSRMTGETLEILGNLANRIMGGSRSIPANNGRGILHLAEPCD
                     ELLCHIQMQVGRPADLDQVRDAYVSRVLAALRRGADDEALEQAAAMQDAFASRPFWSR
                     FFDRPAAWRSIGESLPVRPLVEEARAAKATLESCRLVLALRLYRDRHGEWPERLDALA
                     PEWLPEIPEDPFTGESFAYQREGNGWLVQAVHPDARAPEPADEGILFHSAEPAEARLL
                     RLITGQATRITGVMDVQMLQRYGLVPKGLSGFLSQVRTNQPLAQALAAVIPAVSDLRG
                     LIRLQETGDTARISDYSFK"
     gene            5819..6526
                     /gene="pyrF"
                     /locus_tag="PKK20_01280"
     CDS             5819..6526
                     /gene="pyrF"
                     /locus_tag="PKK20_01280"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNU98555.1"
                     /translation="MRNPIIAALDVATAERALPLAEQLAPVVGALKVGKELFTSEGPG
                     LVRQLRATGAAVFLDLKFHDIPNTVARAVEAAVRCGVQMLTIHTSGGTAMMRAAEQAA
                     RTTADALGLETPLVLGVTVLTSLDDAALEELGVGDGTAGQVGRLARLGVNSGLRGLVC
                     SPLELRMLRAALPKHVQLVTPGVRPAGAGHDDQKRTMTPQEAIEAGADWLVIGRPICA
                     APNPRAAAERILADLAL"
     gene            complement(6822..7220)
                     /locus_tag="PKK20_01285"
     CDS             complement(6822..7220)
                     /locus_tag="PKK20_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98556.1"
                     /translation="MRRCLGIGGLVFVAVPADPTTGTAASFGCAARVILRKLILRRSV
                     LTARLGFAAAVRGRSGAAPDLFSAAAFFTGGCLTTVFLAVVLDRTAFDAPVGFAATVR
                     RRTPAVPWVFAAGAVGRTALAGRFGFAATV"
     gene            7201..8406
                     /locus_tag="PKK20_01290"
     CDS             7201..8406
                     /locus_tag="PKK20_01290"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNU98557.1"
                     /translation="MPRQRRIPPILLEGDEMPTPLVSGPGRRFSLGPTPPSPALALGA
                     AAERLPAAYGTRRLWLAARDPHWLYANWDLRSEEMEEREGRAVSGHLTLRIHENAVGP
                     APLLQIPLAKGARSWFVRVGRGGVRYIAELGCAQPGLGWQTLLISNPVTTPPDGPATA
                     SEREFATIPVDVPFAAVVEQAVEAARQEEPLAQALDALRNQGFSDLPSPKEVRAPVWT
                     ETQERALAEVLRVDAQRRVWVSSLDVTGLIAERLGPAAEKERGGEVEQPSSPTGGFEA
                     GEAGGISSPSGEGRAIERERGFWFNVNAELVIYGATEPDARVTIAGRVIKLRPDGSFS
                     LRFALPDGEYELPVVALSRSGDDGRSAELEFRRHTEYRGAVGAHPQHPRLTPPHAESI
                     TSAETDQSG"
     gene            8427..10001
                     /locus_tag="PKK20_01295"
     CDS             8427..10001
                     /locus_tag="PKK20_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNU98558.1"
                     /translation="MPGYLALILHAHLPFVRHPEHERFLEESWLYEAITETYLPLADL
                     IERWRKDRLNVRLTLTLTPTLCSMLSDPLLQSRYERRLSALIDLAEKEVHRTCWDAGC
                     HRLAEAYLDRFTRLRARWHACDRNLLGVFRALQDQNRIEIATSAATHAVLPLLGDQPA
                     AMRGQILTARDHYRACFGRDAVGFWLPECAYAPGVEEFLREANLRWFVVDTHGLGNAV
                     PRPRYGTYAPVYTPEGLAVFGRDIESARQVWSRNEGYPGDTRYRDFYRDIGFDLDFDY
                     VRPYLPSPDHRGFTGIKYQSITGGKGGKRCYNPADAQRAVTAQAAHFLEARERQLARL
                     AEVMDQPPILTAPYDAELFGHWWHEGLAFLDAVVRGALDRASDFTFVTPTDYLRRHAT
                     HQVARPSPSTWGEGGHLGVWLNEKNEWIQPHLRVAEQRLTALVRRYPEPGELVDRALR
                     QAARELMLAQASDWPFILHAGTSPEYAQRRVAAHLGRFQELYDMLMAGAVDEAGLREM
                     ETVDNLFPNLDWRYWR"
     gene            10182..10919
                     /locus_tag="PKK20_01300"
     CDS             10182..10919
                     /locus_tag="PKK20_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNU98559.1"
                     /translation="MNLAWVVFAKSEVNLAYVWEKASIEARIIIIILLIFSIFAWSIM
                     ATKAVEMRRARRLNQYFDAEFQAQKTVFAIYDRRVQVDGCPLFTVYQAGCQELDARLR
                     GAEGRPRGERISLTTMEHIKRTLERSVAQQALRLESGLILLAIAVSGSPFLGLLGTVW
                     GVMGAFGGIAQAAAEGSEAGLTAMAPGVAGALVTTVAGLLVAIPSMFGYNWLVHNLRV
                     LTVELDNFAQHLVSNMEIEYLSDNDLR"
     gene            10906..11274
                     /locus_tag="PKK20_01305"
     CDS             10906..11274
                     /locus_tag="PKK20_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98560.1"
                     /translation="MTSDESSDLTQQTIDPGKNQPGVLCGHCDHVNHGGLTECEVCGS
                     QLYTKCSHCGLQAPRVYTRCPKCRHRLRGHSGLSRRSRDKLRRTPSSSDKANRQLIWA
                     AALLLVLVVIATFFVMRYKG"
     gene            11296..11721
                     /locus_tag="PKK20_01310"
     CDS             11296..11721
                     /locus_tag="PKK20_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNU98561.1"
                     /translation="MRRFSQRNTLVTLSDINITPLLDLAFVLLIIFMITTPLLVREHS
                     MNIRLPDGGGASARVDRKDLRTVEISRDGRYMLDRREIALDDIERQLAATFATNRQMV
                     VNIRADEEAMTKHLYPVIDRCTRNGITRLSLRTEAPRRP"
     gene            11718..12644
                     /locus_tag="PKK20_01315"
     CDS             11718..12644
                     /locus_tag="PKK20_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNU98562.1"
                     /translation="MNRLRYNCFFVSTAMHGLLLVLFVVGSGFQAMDREPESVELPLL
                     DFVPMRLVDAAVAGGGDPRGGAAPERSPVQTPPPRPDPPKPRPVAPPKEEEAVKPTLV
                     VNESEPKPAKVETQKPPEPDPKDFSEKAPTKPKRKIEVSKNLVTRRPQDDAAARKRAA
                     EKAEAEAAQQAERARQARSGQIRSVLSTLQGNLSGRSVVAVPYGPGGGGETYAGYGLY
                     VRMIYERAWRPPVEVPERRSVVTARVVIARDGSVVSAEIVERSKAPALDQSVTSALGR
                     VTTIGRPFPEGSTDERKTFLIEFDLKARQGTG"
     gene            12641..13828
                     /locus_tag="PKK20_01320"
     CDS             12641..13828
                     /locus_tag="PKK20_01320"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HNU98563.1"
                     /translation="MSRSIYSLGFALVLFGCCAGVGTRAGETERIRISAEAWSTTPPI
                     PIAMSGYSGEVDRVLRFDLEIAGFKFVGESDPSCQYVLKGGNSGQVEGRLTDALKRAT
                     LFAKAYSGGSVRGQAHALADDVVLAVTGKPGIARTKIAFKVTHGQTSEIYVADYDGYG
                     AVPATQDKSIATAPTWRPGHRTLVYTTYKLNNPDIVSHDLDTQERRVVARYSGSNISP
                     AVSPDGRRVAMVLSKAGSPDVYVANIDGTGLKQLTRTREDESSPCWSPDGRMICFATR
                     IDGRRTLAQVPSDGGAIQRIATSGVVNPTEPDWSPDGRYIAFTAQMGAFEICLVKAGG
                     GEAKLLTTGEDPCWAPNSRTLIFTKRGSGGKRFLSLLDVPTKQSKDVLQNIGISSQPS
                     WAR"
     gene            13855..14490
                     /locus_tag="PKK20_01325"
     CDS             13855..14490
                     /locus_tag="PKK20_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNU98564.1"
                     /translation="MKRSAFLNLLLIGLFVAAGGVGCKKTKKPVTPLVSMGSRVPGSG
                     AERRGPITPPTVPEGANVGGTEPTVATPLTDPSTGFTPQADLETIEGMIPDTAHFAGQ
                     TIYFEFDSSVVRTSQYSQCNAVGDDLKTKPEAKLMIDGHCDERGTEEYNRALGERRAL
                     ATREWLIRYGIAPERIFTRSFGEDRPADPGHTEAAWAANRRAEFILLLPPK"
     gene            14590..14763
                     /gene="tatA"
                     /locus_tag="PKK20_01330"
     CDS             14590..14763
                     /gene="tatA"
                     /locus_tag="PKK20_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNU98565.1"
                     /translation="MNSILAVWGLSGGEIVLLLVVVLILFGAKKIPEFAKGLGEGIRE
                     FRKTSKDTPDTKA"
     gene            14769..14933
                     /gene="tatA"
                     /locus_tag="PKK20_01335"
                     /pseudo
     CDS             14769..14933
                     /gene="tatA"
                     /locus_tag="PKK20_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            15112..16113
                     /gene="tatC"
                     /locus_tag="PKK20_01340"
     CDS             15112..16113
                     /gene="tatC"
                     /locus_tag="PKK20_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNU98566.1"
                     /translation="MADPIEEPRLEEDEDGGGPVKSFLEHLEDLRWVLIKSGSAVLIC
                     MVICLLAVNQLVTVLRWPLERARKFNRPTKQQIELYLGTNRLATLPATGSLFDALPLA
                     SNRVTRFEIGPVTVGEQQVLALRSMTNSSMSDVGRGPGLIFLDPMAPFFSSLYIAFFG
                     GMVIAAPLVVYYVGQFIVPALKRKEKKYGLRAFGIGAGLFISGVCFCYFMLLPLALRA
                     AEAYSLWMGVQMPDWRAEAYFSMATKFMLGMGLGFEMPVILLALVKIGILDYAKLAGM
                     RRYMIVINLVLGALLTTPEVLTQVLMAIPLQLLYECSVWIAWYWEWKAKRAQPAAAS"
     gene            complement(16110..17249)
                     /locus_tag="PKK20_01345"
     CDS             complement(16110..17249)
                     /locus_tag="PKK20_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNU98567.1"
                     /translation="MNNPIWVMTSAFKALTFDQVLAQTKRIGAQGMELCVFRRDGTRA
                     DHVATHLDYETFGLDDAKRLIETCQREGMRFSVGAYENLIGGDPAQRLANQNHLLRLI
                     RIAHLCGGDRNNVKVGTFVGYNHDLGVQDRGFEKNLDEFVRVFDPIVRYAESLGVTLL
                     YENCPMEGWRPAGSPTTYNNLPGTLAARKLMYALIPSRAHGETYDPSHDVWQNIDPID
                     VINASDMSRIHRVHIKGTRNLRSPARVHWGALYPMQTVDPARAKKAGVPIPAHDWDRH
                     HYEPMLPGFGGNDSMDWRGFLETLMQRGFENPFVIENEADNSAHTGNLGATVQGFQAA
                     VLCLAPIVWPLVPDDGYRFDAARQPALVDPQVRDIPVKTMNELAR"
BASE COUNT         3071 a         5398 c         6066 g         3169 t
ORIGIN      
        1 tgcgatcggg cccgggattg ttgaccaggg cggaggcgtc ggtgatgagg gagacgatcg
       61 atccgccgcg ctgggcggcg aagtcgccgt tgaccagaaa cgatccgttg tagagccagc
      121 cgcgcgatcc gctcgagcgg acgcgcgacc cgatcttgac gaagtcggcg agcgggcggc
      181 gtacctcttt gcgtccgcgc tgccatccga cctcgatgcc gatgcgttct cctggcaggg
      241 gggtgtgcgg gtcgtcgtgc aggacgttcg tgtttgcggg cttgacgccg agcaggagca
      301 tggcgaggtg gatgtcgcgg ggcgcagcct cggtgcggaa gatgctctcg tgcaccttgc
      361 cggtggtcgt cacgaccgcg tactcgatca ggccggcgcg ttggttgacg gcggctggaa
      421 accgcaccgt gcgcgccgcg cgatcgaggg tgacgagtcc aatccggtga atgccggcgc
      481 gatcgaggcc ggagtcggcg agaggagtcg gttccttgcc gacctgctca gcggccatga
      541 cccgctctcg gaggggcgag ctccgcgagt ccccgatcga ggactgggaa ggtgatcgag
      601 cctgcttttc aaccagggcc tcgtggaact cggccctgcg aaaagaggga ggttcatcgg
      661 gatcagtcgc aatgccgggc gcgccggcgg cgtcgggcgc gagggtgccc agcacgagcg
      721 caaggaggat gccgcgcgag tccgatcccg gccggcggcc gggcaagggc gatggtgggg
      781 tcattggatc gcggcgcggc cgcgggccag ggcgatcaag gcaaagcccg tgccatacgg
      841 ctggtgataa tcgtagaagg ggtaatccca ccaggaaccg tccttttcct ggagcgggag
      901 caggatcgcc gccaatcgtg catggtgtgt ccgggcgcgt tcgggttcga gttcgtcggc
      961 gcagagcgcg gcatagaaat ggccgtagta atagaaatag ccggccacct ggaaccacga
     1021 ttcgtggggg acggggcgct tgcgtccgat ggagagccag ccaatgcggt cgttgaagcg
     1081 ttcgagccac gtctcgagga cggtgtcggt gatgttggtc tcgccccacc agcggagggc
     1141 gatgttgcag gcctgggagc ggcccaggct gcccgcgggg cgattgatgt agcgcatggg
     1201 ccgccacttc agatcctccg cgtaggcata gctaaagtcg ggcttctgct ggcgtttgat
     1261 ggacgccaca ccacgatcga cgagcttctg cggaatctcg attccgatcc gtttcgcttc
     1321 ccggaacgcc accaggacgg tggctgtcac gaagctggtg gactcggcgg ccggctgttg
     1381 cgcgcccacc cgaaagtcgt aatagcccca gccgccgtcg accgattcgt agcggcccag
     1441 cagatcgatc tggccgcgga tgacatcggc gatgcgttgc cgtcggtcgg agtcagggga
     1501 tgcgtggcgg tgcatggctg ccagcgcctg gatgccgtag gcgtgccccc acacattgta
     1561 cagggccacg gcatcggcac ggcgcagtcg tggcaggttg tcgagcagcc aggcctcgcc
     1621 gcgttcgagc gccgccctcg cagtcgggtc gcggtccgct gcgcccgttt cgatcagggc
     1681 ctgcacggcc agcgcggtca cgccggaccg aaacgcctca tgcgcgcccg gcacgggggc
     1741 gaagatgttg aggtctttcg tgcgccgagc ggatccccac gagccgtttg tgttctggct
     1801 cttgagcagg aaccggacac cacggtccac tgcgcggtcc atggccgcgg cgctgggcgg
     1861 cgcgggggtg tcggcgcggg cgggggccgg cgcgaggcag cagaacgccg aacacagcaa
     1921 cagcccgagg accctgagcc tgcatttctc accattcttg cacatcgtga agctggggta
     1981 cgcaaaagaa gtgccaatgc aggctggaga tcgatcgagg atgggcacag ttcatggaac
     2041 gcggcctggt cgagattcaa gcgaagcatt gcatcggcgt ctactcgggc ttcgagagta
     2101 gaatcgtgtc tccttcggca aggccgtcga cgatctcgat ggttttgccg tccgattcgc
     2161 cggtgcggac cgcgcgtttc tcggccttgc cgtcggcaac gcggacgaag acatgtctgt
     2221 cgtcgccttc gccgaacacg gcctggacgg gcgcgaccag ggcgttggct gaactcgtgt
     2281 cggtgagcgt gaccttgcag ttcatgcccg gctgcaattc cagcgggccg ctgggagcga
     2341 ggctgagggt tgcgccgaag ccgccggtca caagggggac acggtccacg gcctcgatct
     2401 tgacgggcac tttgcaattc gggagcgcga caggcgcggc ctcgcccgtc atgccgatcc
     2461 tggtcttgcc caggtcggcc tcgggcacga cggccttgag ctgaaagcga tcggggttga
     2521 caacggtcat gaaaatctcg tatggggcga gcttgccgcc gggaacgagc ttcttgatca
     2581 ccaccggccc ggtggtccat ttgccggctt cgcaggcccc gtacatgacc acgccgtccg
     2641 cgggcgcccg gacatccatg gccgccaggt ccgtcttgag atcgtcgagc cgtttccggg
     2701 ccttcttctg atcgcgggtc agtttctcga cctcgagctc ttttcgcatg agggctttcg
     2761 gaagcgtctc ctcggccagt gcgagcgcca gttcctggtc tcgcttctgg gactggaggt
     2821 tctcggtctc gcgcggaatg gcggtctcga gatcgcgctc ggtgctggcc cggatgttat
     2881 cgaggctgaa cctggctgtc tcgacctcga actcctggcg ttcgaggatg atctcctcgg
     2941 tctgctcggt gaggtcgtcg gcctcgtaca tctgccggag ttgcttgagt tcctcggccg
     3001 cattggcgag gcgttgttcg gcgctcttga cgttgaagtt ggcggacctg atccgcagct
     3061 cccgcccgtg cttctcgaag tactcctggt cctcggccgc gacgcggcgg ctgcggcggg
     3121 cggcggcgag ccgttgcggc gttgtttcct tgaggctggc gagttcggcg cgggccatct
     3181 cgagggcgat cgccgcggcc ggcgcgtcct gttcgaggtc ggctatctgc tcgcgcagct
     3241 tgtcggtatc gagccgcaca agcacatcgc cggccctcac gcgcgctcca tgaggcaccg
     3301 cctccacaac cgtcaggtcc gcccacgcct ttggctcgag gcggacggga tgcatctcga
     3361 cggcttcgag cactgcgtcg aactggacga cgcgtttgat cgcgccgcgc ccggcgacgt
     3421 gcgaagccgg ttccttcggc gcagcgggcg ttgtggcggc gggcgtggcg gccgaaggga
     3481 cgaccgcagc cgtcgagcgg ccgggagatg ttgacttgca gcccacagat gtcagggaaa
     3541 cgacaacgat cagggccgca acagaacaaa gcgatgaacg gggaggacgc atactaccaa
     3601 gcgtgaggca acaacacggc ccgcttctaa agcagacgcg gcaaatacac aagtccgcgt
     3661 tgaagcccct cctccggggt gttgaaatgc gggtaagcgc gtggtactga ctgggcagat
     3721 gggactcggg gcagagacag tgcggatgga gcgccgggcg atcgccgccc agtcggggcg
     3781 cggcccgatg ccgacgcggc gtcgccgcgt gtggcggttg gcgtggcgaa cggcgggcgc
     3841 ggttggcgca ggtctgcttc tgcttggcgc ggtctgtgtc tacggctggc ggttcggctt
     3901 cactccgctg ccgaaaccgt cgccggaaga ggcgtggacg ccgcttcgaa cagcccagcc
     3961 cgccgaattg acgcgcagcg actattggta ctgggtggac gagttcaacc gggcgtcggt
     4021 cgtcaaccgc agaagcgaca tcatccgcgg ctggacgctc tttgactggg ctcaaaccgg
     4081 ccacctgacg gaaaccaacc gcgccgggct tgccgcattc ttcggcggcg acgcgacact
     4141 cgagtcgtgt taccgcggcg cgttggcggc cggaaagacg cacacgaacc tgtgggagcg
     4201 acggcgtgtg ggccgcgacc tggccggcca cacccggttt gtcaatcatg cagcctggaa
     4261 ggccctcgaa gaggagcagg cggggcggac cgttggggcg ctgcggcgcc tgctcgaggg
     4321 ctggcacttg tgcgcgaacg ccggcgccgc aggcttgacg cggcccgaac tgggaacgct
     4381 cgcgggggcg tggcaccgac tggcgctcga aggtccgggg atcgatccgt ccgaggggct
     4441 gcaattgatc gaaggactgg cgcaagtgcg gaaggcactc cctcgggtcg atgttgaatt
     4501 cttgcgcgcc gtgcactcga ggcaggaggt gtttgggcgt gcagcctctg agggtcagct
     4561 gccgcccgcg atcggacgcc agttctgggc ggcattcagc cggatgacag gagagacgct
     4621 ggagattctg gggaacctcg ccaatcggat catgggggga agccgcagca ttcccgccaa
     4681 caatgggcgc ggcatcctcc acctggccga accgtgcgac gaattgctct gccacataca
     4741 gatgcaggtg gggcgaccgg cggatcttga ccaggtccgc gacgcgtatg tcagccgggt
     4801 cctggcggcc ttgcgtcgag gggcggacga tgaggcgctg gaacaggccg cggccatgca
     4861 ggacgcgttc gcgtcgcgtc cgttttggag ccgctttttc gaccgcccgg cggcgtggcg
     4921 ttccatcggg gagtctctcc cggtgcgccc gctggtcgag gaggcgcgcg cggccaaggc
     4981 aacgctcgaa tcgtgtcgtc tggtgctggc cttgcggctc taccgcgatc gtcacggcga
     5041 gtggccggag cgcctcgatg cgctggctcc ggaatggctg ccggagatac cggaagatcc
     5101 gttcacgggg gaatcgttcg cgtatcaacg cgaagggaac ggctggctgg ttcaggcggt
     5161 tcaccccgac gcgcgggctc cggaaccggc cgatgaaggc atcttgttcc attccgccga
     5221 gccggcggag gcccgccttc tgcgtctgat cacgggccaa gccacgcgaa tcacgggcgt
     5281 catggacgtg cagatgctcc agcgctacgg gctggtgccg aaaggccttt cggggtttct
     5341 gagccaggtt cgcacaaacc agcctctcgc ccaggccctc gcggctgtca tcccggccgt
     5401 gtccgatctg cgcgggctga tccgcctcca ggagaccggg gacacagccc ggatttcaga
     5461 ttactccttc aagtgatgag aaatccggcc tagcgcgcat attcccttac accccgtcgg
     5521 tgcacagggc acccagactt cgcaagataa cttctatgcg ggctcgagaa agaaaaccga
     5581 agactccaaa acagaggccg atgtcggcaa gaacgcaatg cgccttgctg ccagggaacc
     5641 atccgggcta gagttgctca ctcgatgaaa tctgtagtaa ttgagtcaag tttttgcgct
     5701 gcgcagcggc gccgcttagc gaggcgtcgg ctgccggtgg gcatggccaa aacccagcgc
     5761 gcacgaactg gtgacatgct cggtcggtct gaggcagagc ctcgctaaac tgccgccgat
     5821 gcgcaatccg atcattgcgg ccctggacgt ggcgacggcg gagcgggcgc tgcctctggc
     5881 ggagcagttg gctcctgtgg tcggcgcgtt gaaggtcggc aaggagcttt tcacgagcga
     5941 agggccaggg ctggtccgtc aactgcgcgc aacaggcgcg gccgtgtttc tcgacctgaa
     6001 gttccatgac atcccaaaca ccgtggccag ggccgtcgag gcagcggtcc ggtgcggcgt
     6061 ccagatgctg accatccaca ccagcggcgg cacggccatg atgcgggcag cggagcaggc
     6121 ggctcggacg acggccgacg ccctcggact cgagacgccg ctcgtgctcg gggtgactgt
     6181 gctgaccagc cttgacgatg cggctctcga ggagctgggc gtcggggacg gaacggccgg
     6241 gcaggtcggg cgcctggccc ggctgggggt gaactccggt ctgcgcgggt tggtctgttc
     6301 gccgctcgag ctgcggatgt tgcgagccgc actcccgaag cacgtgcaac tggtcacgcc
     6361 cggcgtccgc ccggcgggag ctggtcacga cgatcagaag cggacgatga ctccacagga
     6421 agccatcgag gcaggcgcgg actggctggt catcggacgg ccgatttgcg ctgcgcccaa
     6481 tccccgtgcg gcggccgaac gcattctggc ggacctggcc ctataaccgc cggtcttttg
     6541 cgttgctagt acgggaagtc tttctttacc ttcgcgccag caggttcact gctgcttcag
     6601 cttcgatgag acgctatgaa accgcgaaga aaaaccgcct cgacgcgaat cgccggggct
     6661 gaacaggcct cgaaggcagc caagaaccag cccgccacgg ccagtcggcc tactgggcgc
     6721 gttcagcctc gggcggtatt ggcgaagccg aaagcgcccc tcgaggccgc gcggcccaca
     6781 gcagccgcca agacgagtag gggcgcgccc ccagaccagc ctcagactgt tgcagcaaaa
     6841 ccgaaacgtc cggccaaggc cgtgcgcccc acggcgcctg cggcgaagac ccagggcaca
     6901 gccggcgtcc gacgtcgaac cgtcgcggca aaaccgacgg gtgcgtcgaa ggccgtacga
     6961 tccaagacaa cggccaggaa aacagtcgtc aagcagccgc ccgtgaagaa ggcagccgca
     7021 gagaagaggt ccggcgcggc gccggaccgg cctcgaactg ccgcggcaaa accgagacgc
     7081 gcggtcagga cactgcggcg caggatcagc ttgcggagga tcacccgcgc agcacaaccg
     7141 aaagaggcgg ctgtgccggt tgtcggatcc gcggggacgg cgacaaagac aagtcccccg
     7201 atgccgaggc aacggcgcat cccgccgatc cttctcgaag gcgacgagat gccgacgccg
     7261 ctcgtgagcg gtcccggacg ccgtttctcg ctggggccga caccgccatc gccggcattg
     7321 gcgttggggg ccgctgcgga gcgccttcct gccgcgtatg ggacgcggcg gctgtggctg
     7381 gcggcgcgcg atccccattg gctctatgcc aattgggatc tcaggtccga ggagatggag
     7441 gaacgcgagg ggcgcgcggt gtcggggcac cttaccctcc gcatccacga gaatgcggtg
     7501 ggccctgcac cgctgctcca gatccctctg gcaaagggtg cgcgctcctg gtttgtgcgt
     7561 gtcgggcggg gcggagtgcg gtacatcgcg gaactggggt gtgcgcaacc tggtcttggc
     7621 tggcaaaccc tgctcatatc gaaccccgtc accaccccac ccgacggccc cgcgacggcg
     7681 tccgagaggg agtttgcgac gatcccggtc gacgttccct tcgcggcggt ggtcgagcag
     7741 gcggtcgagg ctgcgcgcca ggaggagccg ctggcgcagg ctctggacgc gttgcggaac
     7801 cagggttttt ccgatctccc gagccccaag gaggtgcgcg cgccggtctg gaccgagacc
     7861 caggaacggg ctctggcgga agtcttgcgc gtggatgcgc agcggcgcgt gtgggtgagt
     7921 tctctggacg tgaccggcct gattgcggag cggttgggtc cggcggcgga gaaggagcgc
     7981 ggcggcgagg tcgagcagcc aagttctccg accgggggct tcgaggcggg cgaggcgggc
     8041 ggcatctcga gtccgtccgg cgaagggcgc gccatcgagc gagagcgggg tttctggttc
     8101 aacgtgaatg cggagttggt tatttacgga gccaccgagc ccgatgcacg ggtcacgatt
     8161 gcgggtcggg taatcaaact gcggcccgac ggcagtttca gtctgcgttt tgccctcccg
     8221 gacggcgagt atgagctgcc ggtggtggcg ctgtcgagat ctggcgacga tgggcggagc
     8281 gcggagctgg agtttcggcg ccacaccgag taccgtgggg ctgtcggagc ccatccgcag
     8341 catccccggc tgactcctcc gcacgcggag agcatcacgt ccgccgagac ggatcagtcc
     8401 ggctgacggc tggccgcttt ctcattatgc ctggctatct cgcgctcatc ctgcatgccc
     8461 acctgccgtt cgtccgacac ccggagcacg aacgctttct cgaagagagc tggctgtacg
     8521 aggcgatcac cgagacctat ctgccgctgg cggatctgat cgagcgctgg cgcaaggacc
     8581 ggctcaatgt gcgcctgacg ctgacgctga cgccgacgct ctgttcgatg ctgagcgatc
     8641 cgctgctcca gtcccggtac gaacggcgtt tgtcggcgct gatcgatctg gccgagaagg
     8701 aagtgcatcg gacgtgctgg gacgccgggt gtcatcgttt ggccgaggcc tatctcgatc
     8761 ggttcacccg cctgcgagcc cgatggcacg catgcgaccg gaatctgctc ggggtgtttc
     8821 gcgccctcca ggaccagaat cgcatcgaga tcgccaccag cgcggcgaca catgcggtgc
     8881 tgccgctgct cggggatcaa cccgcggcga tgcgcgggca gatcctcacg gccagggatc
     8941 actatcgggc gtgtttcggg cgggatgcgg tcggattctg gctgccagaa tgcgcttatg
     9001 cgcccggggt cgaggagttc ttgcgggaag caaacctgcg atggtttgtg gtggacaccc
     9061 atgggctggg gaatgccgtg ccgcggccgc gctacggaac ctatgccccg gtgtatactc
     9121 cggaggggct cgccgttttc gggcgcgaca tcgagtcggc gcgccaggtc tggagccgca
     9181 acgagggtta tccgggcgac acgcggtatc gcgatttcta tcgtgacatc ggtttcgacc
     9241 tcgatttcga ctatgtgcgg ccttatctgc cttcgccgga tcatcgcggt ttcactggga
     9301 tcaagtatca gagcatcacg ggcgggaaag gcgggaaacg ctgctacaat ccagccgatg
     9361 cccagagggc cgtgaccgcc caggccgccc acttcctcga agcgcgcgag cgtcaactgg
     9421 cgcgtctggc ggaggtcatg gatcagccgc cgattttgac ggccccttac gatgccgagt
     9481 tgtttggaca ctggtggcac gagggtctgg cgtttctcga tgccgtggtg cgaggcgcat
     9541 tggatcgggc ctcggacttc acgtttgtga cgccgacgga ttacttgcgt cggcacgcga
     9601 cccatcaggt ggcgcggccg agcccctcga cctggggcga aggcggccac ttgggtgtct
     9661 ggctgaacga gaagaacgag tggattcagc cccatctgcg cgtggcggag cagcgactga
     9721 ccgccctggt ccggcgttat cccgagccgg gcgaattggt cgaccgcgcc ttgcggcagg
     9781 cggcccgcga gctgatgttg gcgcaggcga gcgattggcc cttcattttg cacgcgggca
     9841 ccagtccgga gtacgcgcag cggcgcgtgg ccgcgcattt gggtcggttt caggagctgt
     9901 atgacatgtt gatggcgggg gccgtcgacg aggctggctt aagggagatg gagacggtcg
     9961 acaacctttt tcccaatctt gactggcggt attggcggtg agacggagac ccggccgacg
    10021 cagggggcgc gccgactgcg ccgccgatga aacaccggtg aaacattttg atcgcggtcg
    10081 cgggaccgat tattgatact tagggtttgt gcaaaaattg atcggaacta atttttgcac
    10141 agaccattag acaacgaacg ggcgaatgaa ggtgtaatcg gatgaatctg gcgtgggtgg
    10201 tttttgccaa atcggaagtc aatctggcgt atgtctggga gaaggcgtcg atcgaggctc
    10261 ggatcatcat catcatcttg ttgatcttct cgattttcgc ctggtccatc atggcgacca
    10321 aggccgtcga aatgcgtcgt gcccggcggt tgaatcagta tttcgacgcc gagttccagg
    10381 cgcagaagac ggtcttcgcc atctatgacc ggcgcgttca ggtggacgga tgtccgctct
    10441 ttaccgtcta tcaggcggga tgtcaggagt tggacgcgcg cctgcggggc gccgagggta
    10501 ggccccgggg tgagcgcatc tcgctgacaa caatggagca tatcaagcga acgctcgaac
    10561 gctcggtggc gcagcaggcg ctgcggcttg aatcgggtct gattctgctt gcgatcgcgg
    10621 tcagcggctc gccgtttctg ggcctgctcg ggacggtgtg gggggtgatg ggcgcgtttg
    10681 gcggcatcgc acaggccgcg gccgagggca gtgaggccgg cctgacggcg atggcgccgg
    10741 gcgttgcagg ggcgctggtg acgactgtcg cgggcttgct cgttgcgatc ccgtcgatgt
    10801 tcggttataa ctggctcgtg cataatttga gggtcttgac cgtcgaactg gacaactttg
    10861 cgcaacatct ggtgtcgaac atggagattg aatacctgag cgacaatgac ctccgatgaa
    10921 tccagcgatc tgacccagca gacgattgat cccggcaaga accagccggg cgtgctctgc
    10981 ggtcattgtg accacgtcaa tcacgggggg ctcaccgagt gtgaagtctg cggttcacag
    11041 ctctacacca agtgctcgca ctgcggactg caagcgccgc gcgtctacac ccgctgcccc
    11101 aagtgccggc atcggctcag aggccacagc ggcctttcaa gacggtccag ggacaagttg
    11161 cggcggacgc cctcttcgtc cgataaagcg aaccgacagc tcatctgggc ggctgcgctg
    11221 ttgttggtcc tggtcgtcat cgcaacgttc ttcgtgatgc gttacaaggg gtgaggatat
    11281 caggaggcgc gtgacatgcg gcggttctct caacgcaata cactggtgac gctgagcgac
    11341 atcaacatca cgcctttgct ggacctggcg ttcgtgctgc tcatcatctt catgatcacc
    11401 acccccctgc tcgtccgcga gcacagcatg aacatccgac tgcccgacgg cgggggtgcg
    11461 agcgcccgcg tcgatcgaaa ggacctcagg acggtggaaa tcagccggga cggccgctac
    11521 atgctcgaca ggcgggagat tgctttggac gacatcgaga gacagttggc agccacgttc
    11581 gccaccaatc gccagatggt agtcaacatc cgtgccgatg aagaggccat gaccaagcac
    11641 ctctatcccg tcatcgatcg ctgcacgcgc aacggaatca cgcggttgag cctgcgcacg
    11701 gaggcgccga ggcgaccatg aatcgactgc ggtacaactg tttcttcgtg tcgacggcca
    11761 tgcacggcct gctgctggtg ctcttcgtgg tcggatccgg cttccaggcc atggaccgcg
    11821 agccggaatc ggtggaacta ccgctactgg actttgtgcc gatgcggctt gtggacgcgg
    11881 ccgtggccgg gggcggcgat ccgcgcggcg gggcggcgcc cgaacgttcg cctgtgcaga
    11941 cgcccccgcc acgccctgac cctccgaagc cgagaccggt ggcgcctccg aaagaggagg
    12001 aggccgtcaa gcccactctc gtggtcaatg aatccgaacc caagccggcg aaggtggaga
    12061 cgcagaagcc gcccgagccg gacccgaagg atttcagcga gaaggcgccg accaaaccga
    12121 agcgcaagat cgaggtgagc aagaacctgg tgacgcgcag gccgcaggat gacgccgccg
    12181 cacgcaagag agcagccgag aaggcggagg cggaggccgc ccaacaggcc gagcgcgccc
    12241 gccaggcccg gagcgggcag atcaggagcg ttctctccac gctgcagggc aatctgtcgg
    12301 gtcgcagcgt ggttgcggtt ccgtatggtc cgggaggcgg gggagaaaca tacgcgggct
    12361 acgggctgta tgtccggatg atttatgagc gcgcgtggcg tccgccggtc gaggtacccg
    12421 agcggcgcag cgtggtgaca gcccgcgtcg tgatcgcccg tgacgggagc gtggtgtccg
    12481 ccgagattgt ggaacggtcc aaggcgccgg cactggacca atcggtgacg tccgcgctgg
    12541 gccgggtgac aacgatcggg cgtccatttc ccgagggttc gacagacgag cggaagacct
    12601 ttctgattga gttcgatttg aaggcaagac aaggcacggg atgagcagaa gcatatattc
    12661 cttaggattt gcgttggtgc tgttcggatg ctgcgccgga gtcggaactc gggcgggcga
    12721 gaccgagcga atccgcatct ccgcggaggc gtggtcgacc acgccgccga ttccgattgc
    12781 gatgagcggg tactcgggcg aggtcgatcg tgtgctgcgg ttcgatctgg agattgcggg
    12841 cttcaagttc gtcggcgaga gcgatccgag ttgccaatac gttctgaaag gcggcaactc
    12901 gggccaggtt gaagggcggc tcacggacgc tctgaaacgg gcgaccttgt ttgccaaggc
    12961 ctacagcgga ggcagcgtgc gcggtcaggc gcacgccctg gcggatgatg tggtgcttgc
    13021 cgtgacgggc aagccgggca tcgcccgcac gaagatcgcc ttcaaagtca cccacggcca
    13081 aaccagcgag atttacgtgg cggactacga cggctacggc gcggtgccgg ccacgcagga
    13141 caagagcatc gcgacggcgc cgacctggcg tccgggccac cgcacgctcg tttacaccac
    13201 ctacaagttg aacaatcccg acattgtctc gcacgacctc gacacgcagg agcgacgggt
    13261 ggtggcccgg tactcgggct cgaacatcag cccggcggtg tcgccggacg ggcggcgggt
    13321 ggcaatggtc ttgagcaagg cgggcagtcc ggatgtgtac gtggccaaca tcgacgggac
    13381 ggggctcaag caactgaccc ggacccgcga ggacgaatcc tccccgtgtt ggtcgcccga
    13441 tgggcggatg atttgttttg ccacgcgcat cgacggccgc cgcacgctgg cgcaggtgcc
    13501 gtcggatggc ggcgcgatcc agcggattgc gacatcgggc gttgtgaacc caaccgaacc
    13561 ggactggtcg ccggacggca ggtacatcgc gttcacggca caaatggggg cattcgagat
    13621 ctgcctggtc aaggctggcg gcggcgaggc gaagctgctg accacgggtg aggatccgtg
    13681 ctgggcgccc aattcccgga cactgatttt cactaagcgc ggcagtggag gaaagcggtt
    13741 cttatctttg cttgacgtcc ccaccaaaca gtccaaggat gtcctccaaa atatcggtat
    13801 ttcctcccag ccaagctggg ccaggtagac atggtctgtc ggtctaacga atttatgaaa
    13861 cgctcggctt tcctgaatct gctcctgatc ggtttgtttg tggcggctgg tggtgtcggc
    13921 tgcaagaaga ccaagaaacc ggtgactcct ctggtgtcaa tgggatcgcg ggtgccaggg
    13981 agcggcgccg aacggcgcgg tccgatcacg ccaccgaccg tgccggaggg ggccaatgtg
    14041 ggggggacgg agccgacggt tgccacgccg ctcaccgatc cctcgaccgg cttcacaccg
    14101 caggcggacc tcgagacgat cgaagggatg atcccggaca ccgctcattt tgccgggcag
    14161 acgatctatt tcgagttcga cagctcggtc gtccgaacct cgcagtattc ccaatgcaac
    14221 gcggtcggag acgatctcaa gacgaaaccc gaggccaagc tcatgatcga cggccactgc
    14281 gacgagcgtg gcaccgagga gtataaccgc gcgttgggtg aaaggcgcgc cctggcaaca
    14341 cgcgagtggc tgatccgtta cgggatcgct cccgaacgca tcttcacgcg cagtttcggc
    14401 gaagatcgcc cggccgatcc gggccatacc gaggcggcat gggccgcaaa tcggcgggct
    14461 gagttcattc tgctgcttcc gcccaagtaa ccgactcggg cttggcgctc cgcacggccg
    14521 agggaagcga ttttcggcgc tttacacgcc ggagggcgtg gattagcttc ctccagtggg
    14581 ttgacgcata tgaatagcat tctggcagtg tggggattga gcggaggcga gatcgtcttg
    14641 ttgctcgtcg tcgtcctcat cctgttcggg gcgaaaaaga ttcccgaatt cgccaagggc
    14701 ctgggggagg gcatcaggga gttcaggaag acttcgaaag acacacccga cacgaaagca
    14761 tgacttttat ggttcctttt ctcgcagcat ttggtgtggg cggcaccgag ctcattctga
    14821 ttctcctggc ggtgctgatc ctctttggag cgaagaagat tcccgatttc gcgaagggtt
    14881 tgggccaagg catcaaggaa ttcaaaaagg catcgcgcga ggtgcaggag gagatcgagc
    14941 gcgcaggcga ggagttgaac ccaccgcccc cgccccggcc tccactcgcg tcgagttaca
    15001 caccgcccgc cgagtccgtg caccccaccg aacccgccgc tacgtcttcc ggttcgacgc
    15061 cctccgagcc ttcgtccaat ccctgactga gagcggcact gttgtctcct catggccgac
    15121 ccgatcgaag agccgcgtct tgaggaagac gaagacggcg gcggcccggt caagtctttc
    15181 ctcgaacacc tcgaggatct gcgctgggtg ctgatcaaga gcggttcggc cgtgctgatt
    15241 tgcatggtga tctgcctgct cgccgtcaac cagttggtga ctgttctcag gtggccgctc
    15301 gaacgcgccc gcaaattcaa ccggccgacc aaacaacaga tcgagctcta tctgggcacc
    15361 aaccggttgg cgacattgcc cgcaacaggc tccctcttcg atgcgctgcc gctggcgtcc
    15421 aaccgagtca cccgatttga aatcgggccg gtcaccgtgg gcgaacagca ggtgctggcg
    15481 ctccgttcga tgacgaactc ctcgatgagc gatgtgggcc ggggcccggg gctgatcttc
    15541 ctcgatccga tggcgccttt tttctcgtcg ctgtacatcg ccttctttgg cggcatggtg
    15601 atcgccgcgc cgcttgtcgt ctattatgtg gggcagttca tcgtgccggc gctcaaacgc
    15661 aaggaaaaga agtacggcct tcgcgcgttc ggaatcggcg ccggcctttt cataagcggg
    15721 gtctgcttct gctatttcat gctcctgccg ctggcgcttc gggcggccga ggcctactcg
    15781 ctctggatgg gggttcagat gcccgactgg cgggcggagg cctacttcag catggccacc
    15841 aagttcatgc tcggcatggg actcggattc gagatgccgg tgattctgct cgccctcgtc
    15901 aagatcggca ttctcgacta cgccaagctg gcggggatgc gccgctacat gatcgtcatc
    15961 aacctggtgc tcggagcctt gctgacgact ccggaggtcc tcacccaggt ccttatggcc
    16021 atcccgctcc aactcctcta cgagtgttcg gtctggattg cctggtactg ggaatggaag
    16081 gcgaagcgcg cccagcccgc cgccgccagc tagcgcgcca gctcgttcat ggtcttgacc
    16141 ggaatgtccc ggacctgggg atcgaccagc gcaggctgac gcgccgcatc gaagcgatag
    16201 ccgtcgtcgg gcacaagcgg ccacacaatg ggcgcaaggc agaggacggc ggcctggaat
    16261 ccctgcacgg ttgcgcccag attgccggtg tgggccgagt tgtcggcctc gttctcgatc
    16321 acgaacggat tctcaaagcc gcgctgcatc agggtctcga ggaaaccgcg ccaatccatg
    16381 ctgtcgttgc cgccgaaccc cggcaacatc ggctcgtagt gatgccggtc ccagtcgtgc
    16441 gcgggaatgg gcacgcccgc tttcttcgcg cgcgccggat cgacggtctg catgggatag
    16501 agcgcccccc aatgaactcg cgccgggctg cgcaggttgc gcgtgccctt gatgtggacg
    16561 cgatggatac ggctcatgtc ggacgcgttg atgacgtcga tgggatcgat gttctgccag
    16621 acgtcgtggg atgggtcgta ggtctcgccg tgggcgcggc tcgggatcag ggcgtacatg
    16681 agcttgcgcg ccgcgagggt gcccggaaga ttgttgtatg tcgtgggact gccggccgga
    16741 cgccaccctt ccatcgggca gttctcgtac agaagcgtga cgccgaggct ctcggcgtag
    16801 cgcacgatcg gatcgaagac gcgcacgaat tcatcgagat tcttttcgaa gccgcggtcc
    16861 tggacgccca agtcgtgatt gtagccgaca aacgtgccca ccttcacgtt gttccgatcg
    16921 ccgccgcaca gatgggcgat gcggatgagt ctgagcaggt gattctggtt cgccagcctc
    16981 tgcgcggggt cgccgccgat gaggttttcg tacgccccaa ccgagaagcg catcccttcg
    17041 cgctggcacg tctcgatgag ccgcttggcg tcgtcgagtc cgaacgtctc gtagtcgagg
    17101 tgtgtcgcca cgtggtcggc gcgcgtgccg tcgcgccgaa acacgcacag ttccatgccc
    17161 tgcgcgccaa tgcgtttcgt ttgtgcgaga acctgatcga atgtcaaggc cttgaaggcc
    17221 gaggtcatga cccagatggg attattcatg gcgatagaaa ttccacaatt gcgcctcctc
    17281 ggcaagcacg gctttggcgc ggcggacctg aagacctccg ccgcaagacg agccgcttca
    17341 acgcaggtgc gtcagcgaga aagtcagctc cggcagtcga tctccgatct cccatctccg
    17401 cgctctctgt gcctctgtgg ttgatcaccc tcgctgaatg tcaccgtacg gaatgaacca
    17461 cagagacgca gaggacacgg agaaggccat ggccgggcaa tctgtgttcc gtcctgaatc
    17521 ggtgtggagt ccgtccctca cagattcggg gcggaacaca gatggacccc gagactgact
    17581 gaacgggacc tccggcaatc cctgcctcga aagcccccaa ccccagctcg gctacgctcc
    17641 agaggttttc ttccgcagac cgcaattgcc gaccgtaccc ttccgatccg attcccagcc
    17701 gcga
//
LOCUS       DAOQRO010000017        17588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2319_length_17588_cov_5.865112, whole genome shotgun sequence.
ACCESSION   DAOQRO010000017 DAOQRO010000000
VERSION     DAOQRO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17588
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2319_length_17588_cov_5.865112"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKK20_01350"
     CDS             <1..364
                     /locus_tag="PKK20_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNU98568.1"
                     /translation="AAALFRDPRYQSELVVFIDARDEEQYREGHVPGAYQFDHYRPEK
                     QLATVLHVCQLAEQIVVYCTGGDCEDSEFAALALIGVGIPKERFFVYVGGMSEWAAHG
                     LPFEVGERNSGNIGNGEP"
     gene            361..870
                     /locus_tag="PKK20_01355"
     CDS             361..870
                     /locus_tag="PKK20_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNU98569.1"
                     /translation="MSAMPLPGRHKGWNLVAVLTRWLLGGLFLQMGLSKALDPVDFLK
                     LVREYDLTQHPLLLNGIAAALPWFEVFCGVLLLAGVAVRGSALMLLAMLAPFTWVVFQ
                     RALAIQADLGIPFCAVKFDCGCGAGEVYICRKLVENGLLILFSAWLLMGWGRGLCARY
                     ALMKPRAEN"
     gene            922..1392
                     /locus_tag="PKK20_01360"
     CDS             922..1392
                     /locus_tag="PKK20_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNU98570.1"
                     /translation="MKNCKNATAPNSARLANSLNQVLADSYALMALTHLAHWNVEGPN
                     FFGLHTAFQTQYEELFTAIDEIAERVRAVGAYAIGGLGKFAEKAQMKEFVSPLSAEAY
                     VRALIEANDKLLADAAQARDLAGEANDAESQDLMIERITLHQKTVWMLKSVLKS"
     gene            1713..2819
                     /locus_tag="PKK20_01365"
     CDS             1713..2819
                     /locus_tag="PKK20_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNU98571.1"
                     /translation="MASFALASLLCIGGRAADAQPRTLPLGAAAPDFSLPGVDGRTYA
                     LRDFAAARALVVVFTCNHCPTAQYYEGRLMQLAADYRDKGVALVAISPNDPQSVRLDE
                     LGWTDLGDSFADMKLRAKERRFDFPYLYDGETQKASRAYGPVATPHVFLFDRERKLRY
                     AGRIDDSERAQHVKVQYLREALDAVLEGRMPPVAQTKVVGCSVKWSGKADAVKAFMEK
                     LAAEPVSVQSADADALKQLRAGNPSKFRLVTFWATWCAPCVVEFDEFVTMHRMYRHRE
                     FELVTVSVNRPEEHPQVLEFLKQKQASTRNFIFASSDRAPLIDAFDPDWQGVVPYTVL
                     ISPEGKVVSRAVGSIDALEFKREIVRALNDRKPW"
     gene            3171..3254
                     /locus_tag="PKK20_01370"
     tRNA            3171..3254
                     /locus_tag="PKK20_01370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3204..3206,aa:Leu,seq:tag)
     gene            complement(3439..4494)
                     /locus_tag="PKK20_01375"
     CDS             complement(3439..4494)
                     /locus_tag="PKK20_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNU98572.1"
                     /translation="MPRFFEPKVSGTGLLHAAGTLAGLGSAASFFASHSWLLELACHF
                     RLQYALALAAVAVLTRVSPGPNRNNAAAAVRSAHQPRWPSLAYAALALSNLAVLLPSF
                     IPLAPRLPAAASKQWRVVTVNVHTANPRHDLLEAFLRRENPDVILLIEVDARWLTQLE
                     ALTNVWPHTIQTPRDDNFGIALFSRHPFSQARILQLGEAGLPSIEAELRIDTQPVWIF
                     GTHPLPPSSARNARWRDVQLEHAAAHAAARPDPKLLLGDLNTTPWSPVFRRLLARSGL
                     IDTLRGRGYQPTWPSLFFPLWIPLDHCLVSPDLTVLDRRVGPHLGSDHRPVIVDLAFP
                     IDHSPLAMSLWPSTVGH"
     gene            complement(4796..6547)
                     /gene="polX"
                     /locus_tag="PKK20_01380"
     CDS             complement(4796..6547)
                     /gene="polX"
                     /locus_tag="PKK20_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNU98573.1"
                     /translation="MDKDQVAAVLSEIGVWLELKGENPFKTRAYANAARIVESLTEPL
                     ETLVAEQRLSEIKGIGQALQEKITTLVTTGRLPYYEELKASLPAGLTDLLLIPGLGPK
                     KVKALHEQLKIDSVEALEAACHAGQVASLTGFGERSQAKILEGIQYRRQYASRHRLDD
                     ALDLADPILESLRSHPDVIRCSTAGSVRRFKEVIGDIDFLVSSRNPASVIDFFTTKPG
                     VLSVSAKGDTKASVLLEGGIQADLRVVSDAEYPFALAYFTGSKEHNIAMRQRAIQRGL
                     RLNEYGLFRSTEETRDPALRLPCQTEDDIFAALGLASIPPELREDQGEFAAAEQNALP
                     RLLEWTDLRGSLHNHSNWSDGRNALEDIASHMQEIGCAYWAITDHSQASFQANGLDPA
                     RLRQQIKAIEAVNNTLADEGSAFRLLTGTEVDILRDRLDFKDDLLSKLDVVVASLHVP
                     AGGEAENTKRLIRAAQNPYVHMLGHLTGRLLLEREPYKVNAQAVIDACAETGTWIELN
                     SNPYRFDLDWRLWPYARSKGVKCVINVDGHRHEHARFLRLGAGVARKGWLTRADVVNT
                     LPLEKLRQALAAKRQKH"
     gene            6829..7857
                     /locus_tag="PKK20_01385"
     CDS             6829..7857
                     /locus_tag="PKK20_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNU98574.1"
                     /translation="MKTRPSMRAGLALVAALACWATDPWAAAQTPADLEIQLYAGLTI
                     TGTVGSVYSIEYVADLAQANTPGAWQCLDYVQLSSSPCLWTDTSAPATETRYYRAVQM
                     EAPAGMVFIPSGTFTMGSPDDETDRQGDEGPLTAVVLSRWFWMGRYEVTQTEYEALMG
                     NNPSEFRGDSSRPVDSVSWNDAVFYCLKLTQRERAAGRIPPTWLYRLPTEAEWEYAAR
                     AGTTTRFSYGDDPGYFELEDFGWYELNSGEQTHPVGQKLPNAWGLCDMHGNVSEWCQD
                     WYAASLPGGISIDPQGPASGETRVHRGGDWVYWGNGASHCRSAYRNSFFPEFGYSNFG
                     FRVVLSKN"
     gene            complement(7847..8677)
                     /locus_tag="PKK20_01390"
     CDS             complement(7847..8677)
                     /locus_tag="PKK20_01390"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNU98575.1"
                     /translation="MKHNDFPVTYQADPIPRRIMTTQPWNLNRRRFLARAAALASVPF
                     LPTSLPAAEDRPGSKKRVLLVTGVDYPGHPWRETAPALVTMLHQDPRLAVTVIEDPHL
                     LDSPSVHTYDTVLLHFMNWEVPAPGQAARTNLRNFVEAGKGLMLVHFACGAWLDWPEF
                     RNLAGRVWDPKLRAHDPRGPFTVRIMDTEHPITAGLEDFEIDDELYTCLAGDAPIRVL
                     ATARSKVDHIDYPMAFVLNYGKGRVFHSVLGHDAKALAAPGVGDLFRRGCAWTAGLNS
                     "
     gene            complement(8727..9935)
                     /locus_tag="PKK20_01395"
     CDS             complement(8727..9935)
                     /locus_tag="PKK20_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU98576.1"
                     /translation="MFSPRVKTGALMLEGLNALATTIYFSYLYFYMRDRFGFTNLENL
                     SLAALNGLVYSIAVWFAGKFAQTRGYFFALRLGFGTMAVATGIAGLAQSVPGHIGAAL
                     LCSLGMSLTWPTLEAIVSEHESPARLKHLLGVYNVVWAVCGGAAYFMGGALIEQWGHT
                     SIFLVPSTIHFAQVLLTIRLAPAVNPPHPIPVARAPAPPHGEGLASEAERLRSPQPPR
                     RFLQMAWVANPFCYIAINAMLPVIPKIAERFSLGPMLAGFFCSTWFFARTLGFVLLWH
                     WDGWHYRFRWLLSAYVLMVLAFLGVLIGRQLWLLIAAQVVFGLAVALAYYSSLFYSMD
                     VGETKGEHGGFHEAALGAGTCTGPAVGALSLRFFPQHTDMHAWVVAGLLMAGMLWLLA
                     LRYVSPPRSA"
     gene            10235..10549
                     /locus_tag="PKK20_01400"
     CDS             10235..10549
                     /locus_tag="PKK20_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98577.1"
                     /translation="MTVETFEAEINDALRAYDKHIVCIDKPTVDCRETLRSLIEKAII
                     AYETRGPHLRHGIALDKHVTIILSQSQTDRPLCGIYFNLVTPYYQKQAQRAVGAHEGQ
                     QG"
     gene            10595..12634
                     /locus_tag="PKK20_01405"
     CDS             10595..12634
                     /locus_tag="PKK20_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNU98578.1"
                     /translation="MRLRVLWVVLLAAACAGRVEAKTLVLNVATDGNDLWSGELAIPA
                     PSLDDGPLATLSAALDKVRDARYQGLSTVDSFTILVRGGTYRLAEPLVLTPDHSGPSA
                     QQPLVISAYPGEKPILSGGRRIAGWRRSVDKLGCWETDVSDVRAGTWYFRQLFVNGQR
                     KVRARTPNAGFYRIQGESPQDKPIQLKFKAGDIKKEWAEAGDVEVVAYLAWADLRMPI
                     RAVDETNAVATLAGDPRPSNKEANAQYYIENAPDALDRAGEWYLDPKTGVLTYCAEAG
                     EDVQQAEVVAPVLDELVIFRGDFAGKQPVANVVLRGLTFAHTDYRLEAAGYADVQAAI
                     GIAGDIRAEGAVGCVVEDCVFTCLGGYGLDLGKGCQGWRVVGNEFTDLGAGGIRIGET
                     TVRTDAIEECHGHVVTDNRLSRLGRIFASGVGILILQSGNNRVAHNEISDLFYTAISV
                     GWNWGYQETPCRDNLIEFNHLHDIGQNLLSDMGAIYTLGIQRGTVIRNNLIHDVHAFT
                     YGGWGLYTDEGSSYITLENNVVYRCKSAGFHEHYGRENVVRNNIFAFNTEHQLMRSRE
                     EDHISFTFERNIVYFDSGDLLGGSWTNNRFRLDNNVYFDGRPEARPDSMRFAGATLAE
                     WQARGHDIHSVVADPMFYNSRAYNFRLNLDSPALKLEFNPIDLRRVGLRRKGQRQ"
     gene            complement(13185..15938)
                     /locus_tag="PKK20_01410"
     CDS             complement(13185..15938)
                     /locus_tag="PKK20_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98579.1"
                     /translation="MNAFPHVVTSVGRLACLLAVVAMITHCPRIGAAVPPQLEAVVVV
                     FKTHFDIGYTDMASNVVQRYRTTMIDQALEVVDQNRALPLDRQFVWTLPGWPLTQIVG
                     DWPGQNDARRRRVNAAFREGRFVVHALPFTTHTELLEPEDLVRGLGYSSRLCRDAGLE
                     LPRDAKMTDVPCHSWILPTLLRHAGVDFLHLGCNAASSSPRVPILFWWEGPDRSRLLT
                     MYTAESYGTGLVPPADWPHKTWLALIHTGDNHGPPRPDEVKTLLEEAAVKLPGVRVRI
                     GRLSDFSDAILAEKAELPVVRGDMPDTWIHGPMCDPAGARLARNIRPSIAIRETLDTH
                     MRSWIGKSEIGDGRSGMGDGEGIAAAYEKSLLYGEHTWGGAYWWIYGKYIQHYGDKWR
                     QERDAGRFARIESSWAEHTAYIEDAARLTQPALEDQMRALARSVNLEGLRFVVFNPLP
                     WPRDGVVDLPDAMTAGFNTIRSADDGPAQMCIEPGQGLRRFVARSVPPSGYRTYRLSR
                     TPVAEAPRPNPSGQPILSSRFFRATLDPERGVVRSLVDRQSGRELVDRTAPHGFGQYL
                     YERFSSNEVQAYVNAYVKINADWATNELGKPNLPSASEVPYRAGSPRTWKLSIERTRA
                     AQTATLAAPATPDVPAVTTRFILYQEQPCADLELTVHDKAPDPWPEAGWLCLPFKVEQ
                     PRFRLGRLGSIIDPAHEIVPGANRNLYGLDTGLTIAEPGGWGVGLCALDNPLVSLDTP
                     GCWQYSLDFVPRRPTVYVNLFNNQWTTNFRLWNSGTWTARVRIWSVKEAENAADALIA
                     PSLEARFPLQMAWADGPAGPLPARQTGLSVELDGVRVTAFGPNPDGPGTVLRLWEYAG
                     RGGRCGVRLPLGMDPRFVQPVDLRGRPLGAPIPVRARMFHLEMKPFAPASVVIASSTA
                     GLE"
     gene            complement(16504..16797)
                     /locus_tag="PKK20_01415"
     CDS             complement(16504..16797)
                     /locus_tag="PKK20_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98580.1"
                     /translation="MQTKIEKIGDGFGVLLPKELLDACGFGTEATVTLRDKELVVAPT
                     PRRPREGWEDALRAIPKGELERDFAELEAFRETPSEWDVTEWQWPEGDSHEKV"
BASE COUNT         3321 a         5206 c         5890 g         3171 t
ORIGIN      
        1 ggcagcggcc ctgttccgcg atccgcgtta ccagagcgaa ctggttgtgt tcatcgatgc
       61 gcgggacgag gagcaatacc gcgaggggca tgtgccagga gcctatcagt tcgatcatta
      121 tcgccccgag aaacagcttg cgaccgtcct gcacgtctgt cagttggcgg agcagatcgt
      181 ggtgtactgc acgggaggcg attgcgagga cagcgaattc gctgcgctcg ctttgatcgg
      241 cgtcgggata ccgaaggagc gttttttcgt ctacgtaggg ggcatgagcg agtgggctgc
      301 gcatggcctg ccgttcgaag tcggagaacg caacagcggg aacatcggaa acggggagcc
      361 atgagcgcca tgccactgcc cggaagacac aagggatgga acctcgtcgc cgtgctgacg
      421 cggtggctgc tgggtgggtt gtttctccag atgggactga gtaaggcttt ggacccggtc
      481 gattttctca agctggtgcg cgaatacgat ctgacgcagc acccgcttct gctcaacggg
      541 atcgcagccg cgctgccgtg gttcgaggtg ttttgcggcg tccttctgtt ggccggcgtg
      601 gccgttcgcg ggtcggcctt gatgctgttg gccatgctcg cgccgttcac ttgggttgtc
      661 ttccaacggg cattggctat ccaggcggat ctgggcatac ccttctgcgc ggtcaagttc
      721 gactgcggct gcggtgcggg cgaggtctac atctgccgca agctggtcga gaacggtctg
      781 ctgattctct tctcggcctg gctgctgatg gggtgggggc ggggcctgtg cgcccgctac
      841 gccttgatga agccgcgggc agaaaattga ctccggatgc agtttgggat acaccttggg
      901 tcagtttgat tgaacatgct tatgaagaat tgcaaaaacg caaccgctcc gaacagcgca
      961 cgcctggcca acagcctcaa tcaggtgctg gccgattctt acgccctgat ggcgctgacg
     1021 catctggccc attggaatgt cgagggcccc aacttcttcg gcctgcacac cgcattccag
     1081 acgcagtacg aggagctgtt tactgcaatt gatgaaattg ccgagcgcgt tcgggccgtg
     1141 ggcgcttatg ccatcggcgg tctgggcaag ttcgccgaga aggcccaaat gaaggagttt
     1201 gtgtcgcctc tgagcgccga ggcctatgtc cgggcgctga ttgaagcgaa cgacaagctc
     1261 cttgcagacg ccgcccaggc gcgcgatctg gcgggcgagg cgaacgatgc cgagagccag
     1321 gacctgatga tcgagcgcat cacactccat cagaagaccg tctggatgct caagagcgtc
     1381 ctcaagagtt gagaagcgtt catagtcgcc atccgcgttc aatcgtcgaa cctcaacgct
     1441 ccccatggac atcgttcagc gccgccagtt ccctggctgt cgttggcgtc cgtttcgcgg
     1501 ctttagccct gttcgcccaa agacggcacc ccgcgctgca cggaggggat gaggccccaa
     1561 gagcatcgga cagcgcggtg tgcatgcctt tcagataaag aacctgtcac tttatcggga
     1621 gattcttttg catgaccagt tcctcgaatg atccgcgcga tcgatccgga agcgccgccg
     1681 aacagaactc gactggtcgc cgagctgcca ggatggcaag cttcgcgctt gccagcctgc
     1741 tttgcatcgg ggggcgggca gcggatgcgc agcctcgaac gctgccgctg ggcgccgccg
     1801 cgcctgactt cagtctgccc ggtgtggatg gtcggaccta cgcgttgcgc gacttcgcgg
     1861 cggcgcgtgc gctggtcgtg gtcttcacct gcaaccactg cccaaccgcc cagtattacg
     1921 aagggcggct catgcagttg gccgccgatt accgggataa aggcgtggcg ctggtggcaa
     1981 tctcgcccaa cgatccgcag tccgtccggc tcgacgagct ggggtggacc gacctggggg
     2041 attcctttgc ggatatgaaa ctgcgggcga aggagcggcg attcgacttc ccatacctgt
     2101 acgacggcga gacgcagaag gcgtctcgcg cctacgggcc ggtggcgacg ccgcacgtct
     2161 tcctgttcga ccgcgagcgc aagctgcgtt acgcgggcag gatcgatgat tcggaacgcg
     2221 cccagcacgt caaggtccag tacctgcgcg aggctctgga cgccgtgttg gaggggagaa
     2281 tgccacccgt cgcacagacc aaggttgtcg gctgctcggt gaaatggtcg ggcaaagcgg
     2341 acgccgtcaa agccttcatg gagaagctgg cggccgaacc ggtcagcgtg caaagcgcgg
     2401 atgcggacgc gctcaagcag ttgcgggcgg ggaacccgtc gaaattccga ctggtgacgt
     2461 tttgggccac ctggtgcgcg ccctgcgtgg tggagttcga cgaattcgtc accatgcacc
     2521 ggatgtatcg gcaccgggaa ttcgagcttg tgaccgtttc ggtcaaccgt ccggaggaac
     2581 acccgcaagt cctcgaattt ctcaagcaga aacaggcatc cacccgcaac tttatcttcg
     2641 catcctccga ccgggcgcca ctgatcgatg cgtttgaccc tgactggcaa ggggtggtcc
     2701 cctacaccgt cctgatctca cccgaaggca aggtggtgtc tcgggctgtc ggcagcatcg
     2761 atgcgctcga gttcaagcgt gagatcgtcc gtgccctcaa cgaccgcaag ccgtggtaga
     2821 gggaatgaag taaacaccgg tctcgtgccg gtgcaccagc tcttcgaatg acagcatcga
     2881 ccccgccgcg ctttcgcggg cgaggtcggc atcggtcaag gcggcagaat caggatgcac
     2941 ttgaatggct agggcctcgc atctggcccg gtggcgctcc cgccaaaacc ggaagtagtt
     3001 tttgcacaga cccctgagcc ggcgcttgcg ccggtgaagt ggcttgtgca aagtgcccat
     3061 cgatatgcat ggcgttgcgg tgggttgcgc gagcgcgaac cctgtcgaaa acgtcggtga
     3121 ttttggcaag gagttcaccg cggggatgtg agtgtttgaa aaataacgtt gcgcgcgtgg
     3181 cggaatggca gacgcgccag acttaggatc tggttccgaa aggagtggag gttcgagtcc
     3241 tctcgcgcgc accaagtccg ggtggtgtgg ctcgagcgag agacggcctc agcggtgttg
     3301 catcgtcgcg ggcggcagtg atgtggagga ggggcgggag aaaacccgtg acggccatca
     3361 gtcgatggaa aatggccaac gctcaacgtt caacgctcaa cgttcaacgt tcaacgctca
     3421 acgctcaacg ttcaacgctc aatggccaac agtcgacggc caaaggctca tggccagtgg
     3481 cgaatggtcg attggaaagg ccaagtccac gatgaccggg cgatgatcgg agccgaggtg
     3541 cgggccgacg cggcgatcga gaaccgtcag gtcgggtgac acgaggcagt gatcgagggg
     3601 aatccagagc ggaaagaaaa ggctcggcca ggtcggttga tagccgcgtc cgcgcaaggt
     3661 gtcgatgagt ccgctgcgag ccagcagccg acggaagacg ggcgaccacg gtgttgtgtt
     3721 gaggtcgccc agcaggagtt tcggatctgg ccgggcagcg gcgtgtgcgg cggcatgctc
     3781 gagttgcacg tcgcgccagc gggcgttccg tgcactgctg ggtggcagcg gatgcgtccc
     3841 gaagatccag accggctggg tgtcgatccg gagttcagcc tcgatcgatg gcaggccggc
     3901 ctcgcccaat tgaagaatac gggcttgaga gaacgggtgg cggctgaaga gcgcaatgcc
     3961 gaaattgtcg tcgcgcggcg tctggatcgt gtggggccaa acgttggtca gcgcctccag
     4021 ttgcgtgagc cagcgcgcgt cgacctcgat caggaggatg acatccggat tctcgcggcg
     4081 gaggaatgcc tcgagcaggt catggcgcgg gttggcggtg tgcacgttga ccgtgacgac
     4141 gcgccactgt ttcgaggcgg ctgccggtag gcgcggggcg agcgggatga aggaggggag
     4201 caggacggca agattcgaga gggcaagggc ggcgtaggcg agtgaaggcc agcgcggctg
     4261 gtgggcagac ctgactgccg ctgcggcatt gtttcggttc gggccgggcg agactcgagt
     4321 cagcacggcc acggcggcga gggcgagggc gtattggagg cggaaatggc atgcgagttc
     4381 gagcagccac gaatgggatg cgaagaagct cgcggccgag ccgagcccgg ccagtgtgcc
     4441 tgcggcgtgc agcaggcctg tgccgctgac tttgggttcg aagaatcggg gcatcagaat
     4501 ccttgagcgc taaaacatgc cgcagacaag tcaagtttct gtgctgcgtt caaggggtca
     4561 aggatttcta atgagcgagg cgcgggggag aagggcgggg aaacacgggg actcctcgtg
     4621 ggaagtgttg tcattcagcg gtcgaatgcg cacggggacc atgaaccgat ttgtagccgc
     4681 cgacgtgagt cggcgctgat ttctgctgtt gacggagcga gatggcgcgg actcacgtcc
     4741 gcggctacgg aaccacggtt catggaaaga ttggatttgg tctgaggcgc acaggctagt
     4801 gtttctgtcg tttggcggcc agtgcctggc ggagcttttc cagcggcagc gtattgacca
     4861 cgtcggccct ggtgagccat cccttgcgcg ccacgccggc gccgaggcgg aggaatcggg
     4921 cgtgttcgtg tcgatggcca tcgacgttga tgacgcattt gacgcctttg ctgcgcgcgt
     4981 agggccagag gcgccagtcg agatcgaaac gatatggatt cgagttgagt tcgatccacg
     5041 tgcccgtctc agcgcaggcg tcgatgacgg cctgagcatt gaccttgtag ggctcgcgtt
     5101 caagcaggag gcgtccggtc aggtggccca gcatatggac gtacgggttc tgggcggcgc
     5161 gaatgaggcg tttggtattc tcggcttcgc cgccggcggg gacatggagg ctggcgacca
     5221 cgacatcgag tttggacagc aggtcatctt tgaaatcgag ccggtcgcgc aggatgtcga
     5281 cctcggtgcc tgtgaggagg cggaaggcgc tcccttcgtc ggccagggtg ttgttgaccg
     5341 cctcgatggc cttgatctgc tggcgcaggc gcgccgggtc gagcccgttc gcctggaacg
     5401 acgcttgcga gtggtccgtg atcgcccaat aggcgcatcc gatctcctgc atatggctgg
     5461 cgatgtcttc gagcgcgttg cgtccgtcac tccagttcga gtggttgtgc agcgagccgc
     5521 gcagatcggt ccattcgagc aggcggggca gcgcgttctg ctcggcggcg gcgaattccc
     5581 cctgatcctc acgcagttcg ggtggtatgg aagcgaggcc gagcgcggcg aagatgtcgt
     5641 cctcggtctg gcagggcagg cgcagcgcgg ggtcgcgtgt ttcctcggtc gagcggaaca
     5701 ggccgtattc gttgaggcga aggccgcgct ggatggcccg ctgacgcatg gcgatgttat
     5761 gttccttgct gccggtgaaa taagcgaggg cgaacgggta ctcggcatcg ctaacgacgc
     5821 gcaaatcggc ctggataccg ccttcgagaa ggacgctggc cttggtgtcg cctttggcgc
     5881 tgacgctcag gacgccgggc ttggtggtga agaaatcgat cacggacgcc ggattcctgg
     5941 acgaaaccag gaaatcgatg tcgccgatga cttccttgaa gcgccgaacg ctcccagccg
     6001 tgctgcagcg gatgacatcg ggatggctgc ggagcgattc gaggatcggg tcggccagat
     6061 cgagcgcgtc gtccaggcgg tggcgcgatg cgtattgacg gcggtactgg atgccttcga
     6121 ggattttggc ctgcgatctc tcgccaaagc ccgtcaggct tgcgacctgg ccggcgtggc
     6181 aggccgcttc caaggcctcg accgaatcga tcttgagttg ttcatggagg gccttgacct
     6241 ttttcgggcc gagtccggga atgaggagga gatcggtcaa accggcgggc aacgacgctt
     6301 tcaattcctc gtagtacggc aggcgccccg tggtgacgag tgtggtgatt ttctcctgga
     6361 gtgcctggcc gatgcccttg atttcgctca gacgttgctc ggccaccagc gtctcgagcg
     6421 gttcggtgag cgactcgaca atccgggccg cattggcgta agcgcgcgtc ttgaaagggt
     6481 tttcgccctt cagttcgagc cagacgccga tctccgacag cacggctgcg acttggtctt
     6541 tgtccatgcg gtgagtatgc ggagcagtcg ggtcgcttca accgaaaacc ggcaattcag
     6601 cccatgaacc tcaagatgcg cgacgttctg gcaatcgatg gcatcttggg gttcatgggc
     6661 agaatctgcg attcggagca cgatggcctt gctgaatctg ctgccccatg cgagaacatc
     6721 cggtgttgag cccgtgatcg gccgtcttcg ccggacggca gggctccgag gcggcgttca
     6781 cgggttcaag cagcaaacaa tcatcctgat acgtctcttt atgcccttat gaaaacacga
     6841 ccctcgatgc gcgccggatt ggcattggtt gcggctttgg cttgttgggc gacagacccg
     6901 tgggccgcgg cccagacgcc cgcagacctc gagattcaac tctacgcggg gctgacgatc
     6961 acgggcacgg tgggcagcgt ttactcgatc gagtatgttg ccgacctggc tcaggcgaac
     7021 acaccgggcg cctggcaatg cctcgattac gtgcaattgt cgtccagccc ttgcctgtgg
     7081 accgacacat cggcccccgc aaccgagact cggtattacc gcgcggttca aatggaggcg
     7141 cccgcgggca tggttttcat cccatcgggg acattcacga tggggagtcc ggacgacgag
     7201 accgaccgtc agggcgatga aggtccgctg acggctgtcg tcctgagccg atggttttgg
     7261 atggggcgat acgaggtgac acagaccgag tatgaggcgc tgatgggcaa caatccgagc
     7321 gagttcagag gtgactcgag ccgtccggtg gacagcgtga gctggaacga cgcggtcttc
     7381 tactgcctca agctcacgca gcgggagagg gcggcagggc gcattcctcc cacctggttg
     7441 taccggctcc cgaccgaggc ggagtgggaa tacgcggcgc gggcggggac gacgacgcga
     7501 ttcagttacg gagacgatcc gggctatttc gaactggagg acttcgggtg gtatgagctc
     7561 aacagcggcg agcagacgca tccggtcggg cagaaactgc ccaacgcctg gggcttgtgc
     7621 gacatgcacg ggaacgtgtc ggagtggtgc caggattggt atgccgcgag tctgccgggg
     7681 gggatttcaa tcgacccgca gggaccggcg tcgggtgaga cgcgagtgca tcgcgggggc
     7741 gactgggtgt actggggcaa tggcgcgagc cactgccgat cggcctatcg caactcgttc
     7801 ttcccggagt ttgggtacag caacttcggg ttcagggttg tgctgtctaa gaattgagac
     7861 ccgccgtcca cgcgcatccg cgacggaaga ggtcgccgac gcccggggcg gcaagcgctt
     7921 tggcatcgtg tccgagcacg ctgtggaaga cgcgcccctt tccgtagttc aggacgaatg
     7981 ccattgggta gtcgatgtga tccaccttgg atctggcggt cgcgaggacc cgaatcggcg
     8041 cgtcgccggc caggcaggta tagagttcgt cgtcgatctc gaagtcctcg agtcccgccg
     8101 tgatcgggtg ttcggtgtcc atgatgcgga cggtgaacgg gccgcgcggg tcgtgagcgc
     8161 ggagcttcgg atcccagacc cgcccggcca ggttcctgaa ctcgggccaa tccagccagg
     8221 cgccgcacgc gaagtggacg agcatgagtc ctttgccagc ctcgacgaag ttccgaaggt
     8281 tggttcgggc tgcctggcct ggagcgggca cctcccaatt catgaagtgc agcagcacgg
     8341 tgtcgtaggt gtgcacggac ggcgaatcga gcagatgcgg atcctcgatt accgtgacgg
     8401 cgaggcgcgg gtcctgatgc agcattgtga ccagggcagg cgctgtttcg cgccacggat
     8461 ggccggggta atcgacgccg gtgaccagga ggacgcgttt ctttgaaccc ggcctgtcct
     8521 cagcggccgg cagggatgtg gggagaaaag gcacgctggc cagcgcggcg gcgcgcgcga
     8581 ggaatcggcg gcgattcagg ttccagggct gtgttgtcat aatacggcgc gggatcggat
     8641 cagcttggta cgtcacggga aagtcgttgt gtttcatgcg ttctgggggc ttccgcgtgt
     8701 gaaaatgcgt gagaacgctc cggtgttcaa gcagatcttg gcggactgac atagcgcagg
     8761 gccagcagcc agagcatccc ggccatgagg aggccggcca caacccaggc gtgcatgtcg
     8821 gtgtgttggg gaaagaagcg gagcgagagg gcgccgacag ccggcccggt gcaggtgccg
     8881 gcgcccaggg ccgcttcgtg gaatccgccg tgctcgccct tggtttcgcc gacatccatc
     8941 gagtagaaca aggatgaata gtaggccagg gcgacagcca ggccaaacac aacctgcgcg
     9001 gcgatgagca gccaaagttg gcgtccgatc aggaccccca ggaaggccag aaccatgagg
     9061 acgtaggcgc tcaacagcca gcgaaagcga tagtgccaac cgtcccagtg ccaaagtaag
     9121 acgaaaccga gagtgcgcgc gaagaaccag gtcgagcaga aaaaaccggc caacatcggc
     9181 cccagcgaga agcgctcggc gatcttcggg atgaccggca acatggcgtt gatggcgatg
     9241 tagcaaaacg ggttggccac ccaggccatc tgaaggaacc gccgtggcgg ctgaggcgac
     9301 cggagccgct cggcctcgct ggccaggccc tcgccgtggg gcggagccgg cgcacgggcg
     9361 acgggaatcg ggtggggcgg attgactgcc ggcgcaagcc ggatcgtgag aaggacctgg
     9421 gcgaagtgga ttgtcgaagg cacgaggaag atgctcgtgt ggccccactg ctcgatcagc
     9481 gcgccgccca taaaataggc tgcgccgccg cacacggccc agaccacgtt gtaaacgccc
     9541 agcagatgct tgagccgcgc gggcgattcg tgttcgctga cgatggcttc gagggtcggc
     9601 cacgtcaggc tcatgcccag actgcacagc agcgccgcgc cgatatgccc aggcacactc
     9661 tgggcaagac cggcaatgcc tgtggcgacc gccatcgttc cgaagccgag acgcagggcg
     9721 aaaaagtacc ctcgagtctg ggcaaacttg ccggcgaacc agactgcgat cgagtagacc
     9781 agaccgttga gcgcggcgag agagaggttt tcgaggttcg tgaagccgaa ccgatctcgc
     9841 atgtaaaaat acaggtagct gaagtagatc gtcgtggcga gggcgttcaa gccttcgagc
     9901 atcagggcgc cggttttgac gcggggcgag aacatgtgga agcgtggtca gagcggggcg
     9961 ggcggcctca gactgaagaa ctcagaacgc aaacgccggg atgcggcctg atcgaggctc
    10021 gcgatgcggc cgcgtcccaa gagtggcgtc gcagcccaat ggccgcgggc acgcccggac
    10081 ggagccgggc cggacggatg tccggattga atcacggatg tatgccatgt gggccgggac
    10141 tgtggcggaa gggcgtctgg gcgtcaatgt gcgcctcgtt ttcgctcttc actttcgctt
    10201 tgaaatattt cgtcggcccg cctactctgc ggtcgtgacc gtcgaaacct tcgaggcgga
    10261 aatcaacgac gcccttcggg catacgacaa gcatatcgtg tgcatcgaca agcctactgt
    10321 ggattgccgc gagaccctgc gatcgctcat cgaaaaagca atcatcgcct acgagacgcg
    10381 cggtccgcac ctccgccacg gcattgcgct cgacaagcac gtgacgatca tcctgagcca
    10441 atcgcagacc gaccggcccc tgtgcggcat ctacttcaat ctggtgacgc cttattacca
    10501 gaagcaagcc caacgcgccg tcggcgctca cgagggccag caaggctagc ctggaaacca
    10561 aattccagga caagaacaca gaatcatgaa ctcgatgcgc ttgcgagtgt tgtgggtggt
    10621 gctgctcgcc gcggcctgtg cgggccgagt cgaggcgaag accctcgtgt tgaatgtggc
    10681 gaccgatggg aatgaccttt ggtcgggcga gcttgccatt ccggcgccga gtcttgacga
    10741 cggaccgctg gccacgctct cggcggctct ggacaaagtc cgggatgccc ggtatcaagg
    10801 gctctcgacg gtcgacagct tcactattct ggtgcggggc gggacgtatc gcctggcgga
    10861 gccgcttgtg ctcactcccg accactccgg ccccagcgcc cagcaaccgc tggtaatcag
    10921 cgcctatccc ggtgagaagc cgatcctgag cggcggacgc cggatcgcgg ggtggaggcg
    10981 ctcggtcgac aagctgggct gctgggagac ggacgtgtcc gacgtccgcg ccggcacatg
    11041 gtatttccgc cagctcttcg tcaacggaca gcgcaaggtg cgcgcccgga cgccgaacgc
    11101 cgggttctat cgcatccaag gcgagagtcc gcaggacaag ccgatccagc tcaagttcaa
    11161 agcgggggac atcaagaagg agtgggccga ggctggggac gtcgaggttg tggcctatct
    11221 cgcatgggcc gatctccgga tgcccattcg ggctgtggat gagaccaacg cggttgccac
    11281 gcttgcgggc gatccgcggc cttccaacaa ggaggccaat gcgcagtatt acatcgagaa
    11341 tgcgcctgac gcgctcgatc gggcggggga gtggtatctc gacccaaaga ccggcgttct
    11401 gacttattgc gccgaggcgg gggaagatgt tcagcaagcg gaggtggtgg ctcccgttct
    11461 cgatgaactg gtgattttcc ggggtgattt tgcgggcaaa cagcccgtgg cgaacgtcgt
    11521 gctgcgcggg ctcacgttcg cccacacgga ttacagattg gaagccgccg gctatgccga
    11581 cgttcaggcg gcgatcggga ttgcgggtga catccgggcc gagggggcgg tcggatgcgt
    11641 ggtcgaggat tgcgtgttca cgtgtctggg cggatacggg ctcgatctgg gcaaggggtg
    11701 ccagggttgg agggtggtgg gcaacgagtt cactgacctg ggggccggcg gcatccggat
    11761 cggcgagacc acggtgcgca cggatgcgat cgaggagtgt cacggacacg tcgtcacgga
    11821 caatcggctg agtcggcttg ggcggatttt tgcgtcgggg gtggggattt tgattctaca
    11881 gagtgggaac aaccgggtgg ctcacaacga gatttccgac ctgttctaca cggccatttc
    11941 agtcggctgg aactgggggt accaggagac gccctgccgc gacaacctca tcgagttcaa
    12001 tcatctccat gacatcggcc agaacctcct cagcgacatg ggagccatct acacgctggg
    12061 cattcagaga ggcactgtca tccgaaacaa cctcatccac gacgtccacg cgtttaccta
    12121 tggcggatgg gggctctaca ccgatgaggg gagcagttac atcacgctcg aaaacaacgt
    12181 ggtctatcga tgcaagagcg ccggattcca cgagcactac gggcgtgaga acgttgtgcg
    12241 caacaacatc ttcgccttca acaccgaaca ccagctcatg cgctcgcgcg aggaagacca
    12301 catctcgttc accttcgagc gcaatattgt atacttcgat tccggcgacc tcttgggcgg
    12361 cagttggacg aacaaccgat tccggctcga taacaacgtc tatttcgacg gacggcccga
    12421 ggcccgcccc gactcgatgc gttttgccgg agccacactg gccgaatggc aggctcgagg
    12481 acacgacatc cattccgtcg tagccgaccc gatgttttac aattcgcgcg cctacaactt
    12541 ccggctcaac ctggactcgc cggccctaaa gctggagttc aatccgatcg atctacgcag
    12601 ggtcggcctt cgccgcaaag gccagcggca gtgaggctgg cagtgaggct gaatgaccac
    12661 ggcctcgatg acacggaagt ccacccacgc cacatagaaa ctggcgtcgg cgaggtccgc
    12721 ttgcgtgtcc gttgttgtct tgcgcgcttg aatgatgaac ttcccttcga ggaactcgta
    12781 cgagaggaag gcgcgcgagg ccaacggcac cgaggtctcc ggctcgaggt ccgccatctg
    12841 caacaagagg gtgccgtcag cgcccgtttt gccgggaaca accaactcgt agacgcccgt
    12901 gccagtccgg gttgcggcca cttgaccacc ccggccgcca agccggcggc gtgcgcgttg
    12961 ggaccgatcc agcgcgcctg acccttggcg gtcggactgc cggcctcgcc gccgagccat
    13021 ccctggtcgt acgggaacca ggcgatggag aagctgaaat tggcttcagg actcgaaccg
    13081 tgcgtgcccg cgcgctcggt gttcacagcc agtgggttgc acagggacaa gccaggcttg
    13141 accggggttg gcggtcaagc agcattggcg aggaggccca gttttcattc cagtcccgcg
    13201 gtcgaactgg cgatgaccac gctcgcgggc gcgaaaggtt tcatctcgag atggaacatg
    13261 cgcgcgcgca caggtatggg cgcgcccagc gggcgtccgc gcaggtcgac cggttggacg
    13321 aatcgagggt ccatgccaag gggcaggcgc acaccgcaac ggccgccacg cccggcgtat
    13381 tcccagagcc gcaaaacggt gccgggtccg tccgggttgg gaccgaatgc cgtcacgcgc
    13441 acaccgtcga gttcaacgct gaggccggtc tggcgagcag gaagcggtcc cgccggaccg
    13501 tccgcccatg ccatttgcag gggaaatcgc gcttcgaggg agggagcgat cagcgcgtca
    13561 gccgcattct cggcttcctt gaccgaccaa attcgcactc gggccgtcca ggtgccgctg
    13621 ttccagaggc ggaagttcgt ggtccattgg ttgttgaaga ggttcacgta aaccgtcgga
    13681 cggcgcggga cgaaatcgag cgagtattgc caacagccgg gggtgtcgag gctcaccagt
    13741 ggattgtcga gggcgcacag gccgacgccc cagccgccgg gctcggcgat tgtgaggccc
    13801 gtatcgaggc catacaggtt gcggttcgcg ccgggcacaa tctcatgcgc gggatcgatg
    13861 attgacccga ggcgtccgag gcggaaccgc ggctgctcga ctttgaaggg caggcagagc
    13921 caaccggctt ccggccaggg atcgggcgcc ttgtcgtgca ccgtcaactc gaggtcggcg
    13981 cagggttgct cctgatacag gatgaaacgc gtggtgacgg ccggcacgtc cggcgtcgca
    14041 ggggctgcca gagtcgcggt ctgggccgcg cgcgtccgtt cgatgctcaa cttccacgtc
    14101 cgcggcgaac cggcgcggta cgggacctcg gacgcggacg gcagattcgg tttgcccagt
    14161 tcgttcgtag cccaatctgc attgatcttc acgtaagcat tcacgtaggc ctggacttcg
    14221 ttcgaggaga aacgctcgta caagtactgg ccaaaaccgt gcggcgcggt ccgatcgacc
    14281 aactcgcgcc cggactgccg gtcgacaagc gagcggacca cgccgcgctc cgggtcgagc
    14341 gttgcccgaa agaaccggct cgacaggatc ggctgcccgg atggattggg ccgcggcgct
    14401 tcggcgacgg gcgtgcgcga caagcgatac gtccggtaac cggacggcgg caccgagcgc
    14461 gccacgaacc ggcgcaggcc ctgacccggc tcgatgcaca tctgggcagg cccgtcgtcg
    14521 gcggaccgga ttgtgttgaa accggcagtc attgcgtcgg ggaggtccac gacgccgtcg
    14581 cgtggccagg gaagcggatt gaacacgacg aagcggaggc cttcgagatt cacggatcga
    14641 gccagggcgc gcatctggtc ttcgagcgca ggctgggtca ggcgagccgc gtcttcgatg
    14701 tacgccgtgt gctcggccca cgaggactcg atgcgcgcga accggccggc gtcgcgctcc
    14761 tgacgccatt tgtctccgta gtgctggatg tacttgccgt agatccacca gtaggcgcct
    14821 ccccacgtat gctcgccgta cagcaggctc ttctcgtaag ccgctgcgat cccctctcca
    14881 tcccccatcc ccgatcttcc atctccgatc tccgatttcc caatccaact ccgcatgtgc
    14941 gtgtcgagcg tctcgcggat cgcgatcgac gggcggatgt ttcgggccag ccgggcgccg
    15001 gccggatcgc acatcggacc gtgaatccag gtgtcgggca tatcgccccg cacaaccggc
    15061 aactcggctt tctccgcaag gatcgcgtcc gagaaatcgg agagtcggcc gatgcgcacc
    15121 cgcacgccgg ggagcttgac cgccgcctct tcgagcagcg tcttcacctc gtccggacgc
    15181 ggcgggccgt ggttgtcgcc ggtgtggatc agcgcgagcc aggtcttgtg cggccagtcc
    15241 gcgggcggca ccaggccggt gccgtagctc tcggccgtgt acatcgtgag caaccgcgac
    15301 cggtctggcc cctcccacca gaagaggatc gggacgcgcg gcgaactgct ggcggcattg
    15361 cagccgaggt ggagaaaatc gacccccgcg tgccgcagca atgtcggcag aatccaggag
    15421 tgacacggaa catcggtcat cttggcgtcg cgcggcagtt cgagtccggc atcccggcag
    15481 agccgtgacg aatagcccag gccgcgcacc agatcctccg gttcgagcaa ctcggtgtgt
    15541 gtcgtgaaag gcagggcgtg gacgacaaac cggccctccc tgaacgccgc gttcacccgc
    15601 cggcgacggg cgtcgttctg accgggccag tccccgacga tctgcgtcag cggccagccg
    15661 ggcagggtcc aaacgaactg gcggtcgagc ggcagcgcgc ggttctggtc cacgacctcg
    15721 agcgcctggt cgatcatggt cgtgcgatag cgctggacga cattgctcgc catgtcggtg
    15781 tatccgatgt cgaagtgcgt cttgaacacc accaccacgg cttcgagttg cggcggcacc
    15841 gccgcgccga tgcgcgggca atgggtgatc atcgccacca cggccagcaa acacgcaagc
    15901 cggcccaccg acgtcacgac atggggaaag gcattcacaa cggcttgtct acaggcagcc
    15961 ggagcgcgcg tcaaacacgg gcccctggag aaagctgaag ttacccggtt gcagatggct
    16021 tccccgctga ccacaccgct ctgctcaaag cggtccgcgg aaggtcagga cagtgatgtg
    16081 cgaacccacc tccccatgat gtcgagtccg gagaatggcg cccctcgacg acacctccga
    16141 acatggcgac aatcgaccgc gcgccggcgt cgacagcgcc tctaacgcga aattcggggc
    16201 tgttcgatgc cccaaccgct tgccaatacc cattgtccat gttggtcttt ggcgatctag
    16261 tgtgggtgaa aggcgcggat gacccattga ccggcaagaa gtgtcagcac tcggtcagaa
    16321 acgaagaagg tgcctggctg gagaacagcg cacccgacca caaaccgctg attcttgaac
    16381 accactccca agcctctgat ccggaaatca agggtacaga caagctgctc atcccaaacg
    16441 atggttgggc caagatggcc gccccggagg ggtcagattc gggcaggacc ttcctgccag
    16501 acctcaaacc ttttcatggg aatcgccttc gggccactgc cattcggtca catcccattc
    16561 gctcggtgtc tcgcggaagg cctccaattc ggcgaaatct cgctcgagct ccccttttgg
    16621 aatggcgcga agcgcatcct cccagccttc acgggggcga cgcggggtcg gagccacgac
    16681 caactccttg tcccgcaggg tgactgtggc ttcggtgcca aaaccgcagg cgtccagcaa
    16741 ctctttcggc aggagcacgc cgaatccgtc accgatcttc tcgatcttcg tctgcatggt
    16801 cgaaaactag accccggcac cgccgccttc aaccgcattt cctgcctatg cgatccgcac
    16861 ctatctgcct ccgtgtcaaa ggcccgttcg ccgcgagacg cacaacagac caggtcgccg
    16921 cccaagcgag gagttcttgg cccgaaacaa gtatcaccac acgttcctcg ccgcgctgtg
    16981 cctgggcggt tcaatccagg acgctgacag tcggcgaatg ggattcacca cagagcagag
    17041 gacccagcgc ggcacagccg caactgaagc tcggagggcc gagttccgcg agtccctgtc
    17101 cggagaacga ggcagttccc agccagggcc tcgcgtaact cggccctccg gaatcgctcg
    17161 cagcctgcga gcaacttgaa cgattgcagt gcaaagggaa gagtccctca tgaagcgggg
    17221 cggatcttga aggacggatg atctcctgcg tttcccttca tgttccgtct tgattcatga
    17281 ggggtttgga ggtcgcacct ggatgccaac cgaacaggac atcaacgcaa ctcgctccca
    17341 gccaacgttc aacactctcc actctccact ctccactctc cactcccggc tacaaatttc
    17401 atggagtttt ttctgagggg agtgtcagcg gttgtcagac cccctcgttt atgctggcgg
    17461 gcaatgaatg gaagtctaat ccggagaatg gcgcccctcg acgacacctc cagacatggc
    17521 ggtaatcgac catgcgccgg cgtcgacagc gcctctaacg cgaaattcgg ggctgttcga
    17581 tgccccaa
//
LOCUS       DAOQRO010000018        17323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2389_length_17323_cov_5.184793, whole genome shotgun sequence.
ACCESSION   DAOQRO010000018 DAOQRO010000000
VERSION     DAOQRO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17323
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2389_length_17323_cov_5.184793"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PKK20_01420"
     CDS             <1..1235
                     /locus_tag="PKK20_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNU98581.1"
                     /translation="LGGTADALKFVQSEFAAKPDGIGIDCFFGGGPEPYLLLADKGLT
                     TAYRVPDEILSAIPQSANGVELYDPAHRWYGVALSSFGILQNTRVQRRMGLPLARRWA
                     DLANPELRGWVGIADPRNSGTMNNMFEAFLQAYGWRQGWQLLTEIAGNTSKFDRLSSS
                     TAKDVTLGEIAYGFAIDFYGFTQIAVAGRADMTFVLPDDFTAISPDGLCILKGAPHLA
                     AAQRFLHFCLGEQGQRLWFLPRGHPEGPRHHSIERMAVRPDFYRRYRDVSNIAFSPFD
                     LPHVFRYDARLARERREVVAALAGALLVDTHAELQTAWRAMIRRGQIEVESAELGRVP
                     ISEAAGRGLSQGPWKDASFRNRTKQQWQAWAQDKYRTLAERAPAPVPASAGHEPPAVP
                     AAHIRYPGQSSIGTTRTP"
     gene            1244..2476
                     /locus_tag="PKK20_01425"
     CDS             1244..2476
                     /locus_tag="PKK20_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98582.1"
                     /translation="MRVSIRNLTKRFGPAVVLRDVCIEVHDRELFFLLGPSGCGKTTL
                     LRIVAGFYQPDAGEILFADRPMMGVPPQHRNTGMVFQNYALWPHLTVAQNVAYGLEAR
                     GVPARAREQQVREALERVQMTAFSHRSPAELSGGQQQRVALARALVVRPDVLLLDEPL
                     SNLDAKLRLEMRQEIRRIHDESHVTTIYVTHDQKESLSMADRIAVLRDGTIEQIGTPR
                     QIYCAPASRFVADFVGEANWIEANVVATSHAGLELRADCGLFTASPRNGIVPGQPVWL
                     GFRPEAVRFGAQSSNMLQTVVARTHYLGEIEQYLLGLPNGATIKALEQNPMQTRAPGT
                     PLTVHIDPDNLFVMPREIQHPGPDVSQGSAESAIRAPAKPRPEREPGASCRSREWSVM
                     GTVCCSSRSLEPGAVLLL"
     gene            complement(2506..3663)
                     /locus_tag="PKK20_01430"
     CDS             complement(2506..3663)
                     /locus_tag="PKK20_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98583.1"
                     /translation="MNTVRLGIIGLGNIGKYHADYLLNRKVTRCALTAVADASTPLDR
                     YAQESLKTFRDGEALIRSGLVDAVVIATPHYQHTTLGISAIEAGLHVMVEKPISVHKA
                     DAERLIAAHRARPDRVFAAMFQLRTEPRYMKIKRLIEAGDLGTLVRTSWIMTDWYRTE
                     AYYASGGWRATWKGEGGGVLLNQCPHNLDILQWLCGMPQSVRGFCQLGRYHAIEVEDN
                     VTAYWAWANGATGVFVTSTGEAPGTNRLEICGTRGRLVLEDNRLRFTRNEVDMIEFSR
                     SAKSGFARPDIWNIDIPFDNAPAQHATLMQNFVDSILDGAPLVAPGEDGLRSVELANA
                     ILYSSILGQTVELPLDGAAYERTLEGLITQSKFEKKVAAVSDEDFTKSFVR"
     gene            complement(3742..4131)
                     /locus_tag="PKK20_01435"
     CDS             complement(3742..4131)
                     /locus_tag="PKK20_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNU98584.1"
                     /translation="MVLLRVLTGSQAGAITQARCFPFRVGRASSADWRIEAPGVWDEQ
                     FEILLSQADGFLLTANAATLTCVNGERVSQAPLRNGDHIECGDLRIQFWLSPAHQRGL
                     RARETATWVALAGLAAVQAYLFWWLTR"
     gene            complement(4157..4798)
                     /locus_tag="PKK20_01440"
     CDS             complement(4157..4798)
                     /locus_tag="PKK20_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNU98585.1"
                     /translation="MMTTMVQLVRGGWWCALAALLAALSCGCESPSQGGSGYVPAEGA
                     GAGVTPSLSADAVRIGDKVEITFSDIPEVYPIMVQRVREDGSITLPLGVRVEAANKKA
                     TELQDAIVNAYVPKYFKRLSVAVRLEERVFYVGGQVRAPNRYVFLGEMTVLGAIKVAG
                     DFTEFAKRTAVKVTRADGTEIKVDCKKALKNSKYDLPVFPGDKIDVPRRVLWD"
     gene            complement(4834..7008)
                     /locus_tag="PKK20_01445"
     CDS             complement(4834..7008)
                     /locus_tag="PKK20_01445"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNU98586.1"
                     /translation="MDATKPNPISDSQLHFLDYWRIIRIRKTVILAVFLLVVLTTTAV
                     TFVLKPTFMSTARIEVEKQPTDFDPFLRPQVQNQFDPYFIQTEFEKIQSKPILYKVIE
                     SLDLRKTWGQRLKNAVSEPEAYLLLRRNLDVRQSRNTSLIEIRAYSENRDEASAIANE
                     VANVYKESRVEEKQRQTSAGISKLRAKLDEQAEAVAKLQDNVDQLRAELNIPDSINDA
                     SVYQTSLEREKVSAMAQELTRAHSEYVYFLTKLEDLKKLDRTSMREAVLVTMPTEARL
                     GELLGALSLAQQEVARLREDFSNDYPEMKRKLALVEQIDQQVEARLTGILTGLENQVI
                     SLKARRDELQKAVDDGKKLVVEEAKRSREYWSSKRELENKQKIRDAILMRMLQEEVDA
                     ELPKESMVRIVDPAEPALRPVRPNIPLNIALGIMVGLVMGVGLAFFIEYLDTSVKTID
                     DVERSLSAPVLGVIPQNVGILVNEGPDSPHGEAYRVLRTNILFSRKEPGANTLTVVSG
                     GAGEGKSTTILNLATVFAQNGQRVLVVDSDLRRPSLHKFLAVSNSIGLTNYLMKQNTL
                     EEVIQTTKLPMMDFLASGKLPSSSLGILNSTRMKEFIKDVQRRYDFVFFDSPPIMGVS
                     DASILASEVDLSVLVVQYRKYPQAMTLRAKQMVEKVGGTMLGVVLNNINISQDSYYYY
                     YSGYYYDYYSKGEYGESKSSEDDESRKAAPKKPAEAELKTKY"
     gene            complement(7084..8376)
                     /locus_tag="PKK20_01450"
     CDS             complement(7084..8376)
                     /locus_tag="PKK20_01450"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNU98587.1"
                     /translation="MNKLITFASLAAVGALSLQGQAVDGSGRPWSVSAKLRGFYDDNY
                     RTTPSNLTPEAKEYLGNVDKESWGINFAPGIKYVLLRDQTTLTASLDYDLRWYEAQPS
                     SKEFDHTVRAEIAGSHAFSERFRLDFFESPVYSEEPSILEPNGGQASYVRTDGNAWRN
                     YAGIGLTTSLTEQLGSRLGYSNTYYNYQDDGAGSISALLDRMEHLASADLRWQFQPAI
                     IGLVGYQYGYFDYTSGDQLWYTDTPDRTLPESDARNQESHYGFLGVDYTALPGLTAQI
                     RAGVNYARYPNAHVDDVTAPFVDAALAYEYMEGSKVSVGVKHDLRPTDVAIARTDDLS
                     SFTVSQEATTVYAFVAHRIFPKLIGTLRGSWQHGTFEEGMYDGDSDNFYTVDVNLAYE
                     ITANFAAEVGYAYDKLDSDIPMRDYDRNRFYVGVKATY"
     gene            complement(8639..9529)
                     /gene="rarD"
                     /locus_tag="PKK20_01455"
     CDS             complement(8639..9529)
                     /gene="rarD"
                     /locus_tag="PKK20_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNU98588.1"
                     /translation="MSSARKGIWLGLGAYLLWGLLPVYWKALRTVPAPEILMHRIVWS
                     FGFLALLVILSRQGRTLLAAATQRRTLAIYLGAALLLGLNWFVYIWAVNHDRIVETSL
                     GYYINPLVSVALGVVVLRERLGAIQWMAVGIATLGVLFLTWQYGAFPWVAVVLALSFA
                     CYGLIKKTAPLPALPGLTIETALLWLPAAGYLGALETQGIGRFGQSGGWGDSLLVLTG
                     VVTALPLLLFASAARLVKLSTLGLLQYVSPTCGLILGVAVYGEPFPRARALGFGIIWL
                     ALAVYWLASRAAHRPTLGAS"
     gene            complement(9595..11184)
                     /gene="purH"
                     /locus_tag="PKK20_01460"
     CDS             complement(9595..11184)
                     /gene="purH"
                     /locus_tag="PKK20_01460"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNU98589.1"
                     /translation="MQPIRRALLSVYDKTGLLPFARVLVGAGVELLSTGGTARALREA
                     GLPVSELGEFTGFPEMLDGRVKTLHPKVHGGLLYLRGHPEHEATADRHGIRPIDLVAV
                     NLYPFEETVVRPGVTRADVVENIDIGGPAMLRSAAKNHEHVTAIVDPADYEEVAAQVQ
                     ARGGTTLELRRRLAVKVYARTAAYDGAIAAHLPHWFGAGGGTDLAAVGRLGGSAPPDR
                     LIVDAPRVQALRYGENPHQPAALYGRFADHFRQIHGKELSYNNILDLTSAATLISEFR
                     GDAPTLAILKHTNPCGVGRGASLCEAWEKALATDRQAPFGGIIAVNQPMDGPCATAIA
                     EIFSEVVVAPSFTPEAVAVLQQKRNLRLIEVSMDSHATAGGEIRGVGFESFLWQQPDR
                     ATVRARDQVKVVTKRAPSEDEYQALLFGWRVVKHVKSNAIVYAAADRTLGIGAGQMSR
                     VDASRIAVWKAGEAGLSLRGSVVCSDAFFPFPDGLVAAAEAGATAAIQPGGSKRDAEV
                     IAAADARGVAMVFTGIRHFRH"
     gene            complement(11239..12699)
                     /gene="purF"
                     /locus_tag="PKK20_01465"
     CDS             complement(11239..12699)
                     /gene="purF"
                     /locus_tag="PKK20_01465"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNU98590.1"
                     /translation="MQQHPKDHCGVFGVYGHPNAAELTYYGLYALQHRGQESAGIVSC
                     DGRQFRVHRGMGLVPQVFSGRILRDLEGDMAMGHTRYSTMGSSHIRNAQPLTADSARG
                     QIAIAHNGNLTNAVQLREELEAQGTLFQTTVDSEIILHLMARPGPRGAASSLVQTLRR
                     IEGAYSLILQTEQELIGVRDPHGFRPLSLGRLGDAWVLSSETCAFDLIQATFVRDIAP
                     GEILVINREGLRSIQAFPDHPRRAFCIFEYVYFARPDSTIAGRNVHRVRVAMGRELAR
                     EYPRQADLVVPVPDSGNCAALGYSLESGIPFEMAFVRNHYVGRSFLQPSQLIRDFNVR
                     VKLNLIREVVAGKRVIIVDDSIVRGTTCRQRVANLKEAGAREVHVMVSCPPHVNPCVY
                     GIDFPDRSKLMAANHSVDEICRYLKADSLAYLSQAGMVRATGLPASAFCLACYDGDYP
                     VRYDLALDKQIIQRRRARVQSLGELLAREDLQPRLL"
     gene            12915..13751
                     /locus_tag="PKK20_01470"
     CDS             12915..13751
                     /locus_tag="PKK20_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNU98591.1"
                     /translation="MAEYKVQFEVFEGPLDLLLYLVRKEEVDIYQVNLTRLATQFLEY
                     VDLMRQLDLDIAGEFLVMASTLMYLKSRELLPVDQQAISLDEDEDSDDPRWELIRKLV
                     EYRKFKDAASQLQALELQQEQIYPRQPAKPEFEPVPARIEVSLFDLIGAVSAIMKRIQ
                     EREETRDIFEDKWIVSERIDFIRTLLGSRPAFTFADLFGQVTSRSEVVATFLALLELI
                     RLHHAVAFQREPFGEIEIAAAPTPPPGPAPGPSLDSDPAAGVSDTHAAVCPDDSAPPP
                     FA"
     gene            13748..14545
                     /gene="scpB"
                     /locus_tag="PKK20_01475"
     CDS             13748..14545
                     /gene="scpB"
                     /locus_tag="PKK20_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNU98592.1"
                     /translation="MIELKSILEAVLFSAQKPLRALELKNILTAAAEQSDDEAARAQK
                     RVKTADIDSALEQLREEHAAADRSFSLACIADGWQFVSKPAYAPWLRVLAGERARPAR
                     LSAPALETLTIIAYRQPVTRAEIEQIRGVSIDGVMQTLLERNLIEAVGRAEVVGRPMQ
                     YGTTRLFLEYFGLRSLDDMPTADELRRIPVDRPPSLLTVEPGLATAPPEQLTLQPQEQ
                     SEPCPHATSQNPAAEPSPALDRQPAHDAPSEPVPAPGGENLQSPLQE"
     gene            14542..15603
                     /gene="pheA"
                     /locus_tag="PKK20_01480"
     CDS             14542..15603
                     /gene="pheA"
                     /locus_tag="PKK20_01480"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNU98593.1"
                     /translation="MSLLEHRTAIDRTDQQIVRLLNQRTRHVLAIGSIKLKSGQEIYA
                     PHRERTVLDRIAKMNRGPLSSEAIRAIYREIMSSALAVEKPMTIAYLGPEATFTHQAA
                     IQRFGTSLRYAALKTIADVFSEVAKRHADFGVVPIENSTEGVVTHTLDMFMDSDLKIV
                     AQIVIPIQHCLMGRARRQQIRRLFVHPQSLAQCRGWLQRNLPNAEIIETSSNARSAEL
                     AAGARNAVAIAGLLAAEKYGLPVIEHNIQDNAANATRFLVLGRQSPPRSGRDRTSLMF
                     CIADRVGALHGAIAPFRKYRINMTKIESRPSKRKAWEYYFFVDCDGHIEDPRVAKMLE
                     ALGRHCVFVKVLGSYPNTD"
     gene            15703..16476
                     /locus_tag="PKK20_01485"
     CDS             15703..16476
                     /locus_tag="PKK20_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNU98594.1"
                     /translation="MQSSLPSVSILLAALTASTLIGQDVKPDSLQQARSFQRSVTRTV
                     GADYLLFFPRGYDEAVEKRWPLILFLHGAGERGTNIWLVAKHGPPKLVTAKPDMPFII
                     ASPQCPPGQTWSNDTVLALLDELLANHRVDPERVYLTGLSMGGYGTWSLALAAPERFA
                     AVVPICGGGDPIKLLLADPRRAQALKSLPIWAFHGAKDPVVKLEESERMVDALKRFGC
                     SEVQLTVYPEAGHDAWTQTYENPKLYDWLLAHRRTASSR"
     gene            complement(16616..>17323)
                     /locus_tag="PKK20_01490"
     CDS             complement(16616..>17323)
                     /locus_tag="PKK20_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98595.1"
                     /translation="TEDYIEYILNRRLGCWYLGVNVPLCMVMRRLQEPYDGSAAASAG
                     LRLPVTYFERDFISGFPANQLPRSRYENPEFANPFARLFGKAAAANLIVGRGDPMSQQ
                     VVFDVGDEIVVEDEQGMPKELIVCDHTGSFVCYQQTLLELAPAYAKPVNDRSDKLAHP
                     REFALAYLEGFDVWFRHIQGHYRDHRRAFDALFKHMPPDPEGNFSYRWECVLRRLNEA
                     DPAELVRAIRSRIAVLP"
BASE COUNT         3353 a         5901 c         4937 g         3132 t
ORIGIN      
        1 acctgggcgg cacggcggac gccctcaaat tcgtgcaatc cgagtttgcc gccaagcccg
       61 acgggatcgg tatcgactgc ttctttgggg gcggccccga accgtatttg cttctggccg
      121 acaagggtct cacgacggcc tatcgcgtcc ctgacgagat tctgtcggcc attccccaaa
      181 gcgcgaatgg cgttgaactc tacgatcctg cgcaccgatg gtatggggtc gctctgtcca
      241 gcttcggcat tctgcaaaac acacgtgtcc aacggcgcat gggcttgccg ctggccaggc
      301 gttgggcgga cctggccaat ccggagttgc gcggctgggt tggcattgcc gacccgcgga
      361 acagcggaac gatgaacaac atgttcgagg cattccttca ggcctatggg tggcgacagg
      421 gatggcagtt gctgactgag attgccggaa acacatccaa gttcgaccgc ctttcgtcga
      481 gcaccgccaa agacgtcacg ctgggcgaga tcgcctacgg cttcgccatc gatttctacg
      541 gcttcacaca gatcgccgtc gctggccgcg ccgacatgac cttcgtgctt cccgacgatt
      601 tcaccgcgat cagccccgac gggctctgca tcctcaaagg cgcgccgcac ctcgctgccg
      661 cccagcgatt cctccatttc tgcctcggcg aacagggcca gcgcctctgg tttctgccgc
      721 gcggccatcc cgaggggccg cgtcaccact cgatcgaacg catggcggtg cgccccgatt
      781 tctaccgacg ctatcgcgac gtcagcaata tcgccttctc gcccttcgac ctcccacatg
      841 tctttcgcta tgacgcccga cttgcccgcg aacgccggga ggttgtcgct gctctcgccg
      901 gcgcgctgct ggttgacacc cacgccgagc tccagaccgc atggcgcgcg atgatccgcc
      961 gcggccaaat cgaggtcgaa agtgcggagt tgggccgcgt gccgatcagc gaggcggcag
     1021 gacgcggtct gtcccaaggg ccgtggaaag acgcctcttt tcgcaaccga acgaaacagc
     1081 aatggcaggc ctgggcgcaa gacaagtatc gcacactggc agagcgagcg ccggcaccag
     1141 tcccggcctc tgccggccac gagccgccgg cagtgcctgc agctcacatc cgttaccccg
     1201 ggcaaagctc gatcggaacg acccgaaccc cctgaccttc cccatgcgcg tctcgatacg
     1261 aaatctcacc aagcggtttg gccctgccgt ggttctgcgg gatgtctgca tcgaggtcca
     1321 tgaccgtgag ttgttctttc tgctggggcc ctccggttgc ggaaagacca ccttgctccg
     1381 gattgtcgct ggattctacc aacccgacgc tggagaaatt ctctttgcag accgtccaat
     1441 gatgggcgtg ccaccccaac accgcaacac cgggatggtc tttcagaact acgcactgtg
     1501 gccgcatctg acagttgcgc agaatgtcgc gtatgggctg gaggcccgcg gcgtcccggc
     1561 tcgggctagg gaacagcagg tccgcgaggc gctcgagagg gttcagatga cggccttctc
     1621 gcatcgttcg cccgccgaac tgtcgggagg ccaacagcaa cgggtggctt tggcccgcgc
     1681 cctcgtggtt cggccggatg tgttgctgct cgacgagccg ctctccaacc tcgacgccaa
     1741 gctgcgcctc gagatgcgcc aggaaatccg acgcattcat gacgaatcgc acgtcacgac
     1801 aatctacgtc acccatgacc agaaggagtc actgtcgatg gccgaccgaa tcgccgtcct
     1861 cagagacggg acgatcgagc agattggcac accccgacaa atctactgcg ctccagccag
     1921 ccgattcgtc gcggacttcg tcggtgaagc caactggatc gaggccaatg tcgtggcgac
     1981 atcgcacgcc ggcctcgagc ttcgcgccga ttgcggtctg tttacggcgt caccgcgtaa
     2041 cgggattgtc cccggacagc cggtctggct cggtttccgc cccgaagcag tccgattcgg
     2101 ggcccagtcg agcaacatgc tgcagactgt cgtcgcccgg acgcactacc tgggcgagat
     2161 cgaacaatac ctcctggggc tgcccaatgg ggccacaatc aaagccttgg aacagaatcc
     2221 catgcaaact cgagcccccg gaactccgct gaccgtccac atcgaccccg acaacctgtt
     2281 tgtcatgccg cgcgagatcc agcaccctgg cccggatgtt tcacaaggtt ccgcggagtc
     2341 cgccatccgg gctccggcaa aaccgcgccc agaaagggaa ccgggagcat cctgtcggtc
     2401 ccgagagtgg tctgtcatgg gaacagtgtg ttgcagttct cgatctctcg aacctggcgc
     2461 ggttctgctt ctctagcccg catagtggtc ctcttgtgaa gaaggtcacc gcacaaagga
     2521 cttcgtgaaa tcctcatcgc tgaccgctgc gaccttcttt tcgaacttgg actgtgtgat
     2581 caagccctcg agcgtccgct cgtatgcggc gccgtccagc gggagttcga cggtctggcc
     2641 cagaatcgac gaataaagga tcgcgttggc aagttcgacg gagcgcagac cgtcttcgcc
     2701 gggagccacc aggggcgcac cgtcgagaat ggagtcgacg aagttctgca tcagcgtcgc
     2761 atgctgggca ggcgcgttgt cgaacgggat gtcgatattc cagatatcgg gccgcgcaaa
     2821 acccgacttc gccgaccggc tgaactcgat catgtcgacc tcgttgcgtg tgaacctgag
     2881 gcggttgtct tcgaggacga gtcggcctcg agtgccgcag atctcgagcc ggttggtgcc
     2941 gggcgcttca ccggtcgagg ttacaaacac ccccgtcgct ccgtttgccc aggcccagta
     3001 ggccgtgaca ttgtcctcga cctcgatggc gtgataccgc ccgagttgac agaaaccgcg
     3061 caccgattgc ggcatgccgc acagccactg gagaatgtcc aggttgtgcg ggcattggtt
     3121 gagaagcacc ccgccgccct ctcccttcca cgtggcgcgc cagccgccgc tggcgtaata
     3181 cgcctccgtc cgataccagt cggtcatgat ccagcttgtc cgcaccaaag tccccagatc
     3241 gcccgcttcg atcaaacgct tgatcttcat gtaccgcggc tcggtccgaa gctggaacat
     3301 ggcggcaaac acccgatctg gcctggcccg gtgcgccgca atcaaacgct cggcgtctgc
     3361 tttgtgcacc gaaatcggct tctcgaccat cacatgcagg cctgcctcga tcgcggaaat
     3421 ccccagggtg gtatgctgat agtggggcgt cgcgatgaca accgcatcca ccaaaccgga
     3481 ccgaatcagc gcctctccgt ccctaaacgt cttcaacgat tcctgcgcgt aacgatccag
     3541 cggggtggag gcatcggcca cagcggtcag ggcgcaccgc gtcaccttcc gattgagcag
     3601 gtagtcggcg tggtactttc cgatgttgcc cagtccgatg atcccgagtc gcaccgtgtt
     3661 catgggacga gagcgtagcg ggctcgggct cgaaaggcga gcaaagaccc gcatcgctcg
     3721 atgtgtgcgg cggcgccgag tttatcgggt caaccaccag aagagatacg cttgaacggc
     3781 cgccagaccg gccagggcaa cccacgtggc cgtctcccgc gcccgcagtc cgcgctggtg
     3841 ggcagggcta agccaaaact ggattcgcag atcgccgcac tcgatgtgat cgccatttcg
     3901 caggggagcc tgggaaacgc gctcgccgtt gacacatgtg agggtcgccg catttgctgt
     3961 gagaagaaac ccatccgcct gagagaggag gatctcgaac tgctcatccc agacccccgg
     4021 ggcttcgatt cgccagtccg cgctcgaagc acgccccact cgaaacggaa aacaccgcgc
     4081 ctgcgtgatc gcccctgcct ggcttcccgt caggacgcgc agaaggacca ttttgttacc
     4141 tcccccgcgc gatcggctaa tcccaaagca cgcgacgcgg gacgtcgatc ttgtctccgg
     4201 gaaagacggg cagatcgtac ttcgagttct tcagcgcttt cttgcagtcc actttgatct
     4261 cggtgccatc agcacgggtg acctttacag cagtgcgctt ggcgaattcg gtgaagtcgc
     4321 cggcgacttt aattgccccc aaaaccgtca tttcgcccag aaagacatag cgattcggtg
     4381 ctcgcacctg cccgccgacg tagaaaacgc gctcctcgag ccgcaccgcc acgctcagtc
     4441 gcttgaagta tttggggaca taggcgttga cgatcgcatc ctgcagctca gtcgccttct
     4501 tgttggccgc ctcgaccctg acgccgagcg gcaacgtgat gctcccgtct tccctgacac
     4561 gctggaccat gatggggtag acttccggta tgtctgaaaa cgtgatttca accttgtccc
     4621 caatccgcac cgcatccgcg ctcaaagacg gcgttacccc ggcgccagcg ccttccgcgg
     4681 gcacatagcc gctgccgccc tgactgggac tctcgcaccc gcagctcagc gccgcgagca
     4741 gcgccgccag cgcgcaccac catccccctc tgaccagttg gaccatcgtc gtcatcatcg
     4801 ccacatcctt caactccgcc cgagccaagg caatcagtat ttcgtcttca actccgcttc
     4861 cgccggcttc ttgggggctg ccttgcgaga ctcgtcgtcc tcgctcgatt tggattcacc
     4921 gtactcgccc ttcgagtagt aatcgtagta atagccgctg tagtagtagt agtaggaatc
     4981 ctgcgagatg ttgatgttgt tgagcacaac accgagcatc gttcctccga ccttctcaac
     5041 catctgcttg gcccgcaagg tcatcgcctg cggatacttg cggtattgga ccaccagaac
     5101 cgacagatcc acctcgctcg ccagaatcga ggcgtcgctc acgcccatga tcggaggaga
     5161 atcgaaaaac acgaagtcgt atcgccgctg gacatccttg atgaactcct tcatccgcgt
     5221 cgagttcaga atccccaggg aactgctggg caacttgccg ctcgccagga agtccatcat
     5281 cggcaatttg gtggtctgga tcacctcctc gagcgtgttc tgcttcatga ggtagttggt
     5341 cagcccgatg ctgttgctca ccgccaggaa tttgtgcagg ctggggcgcc tcagatccga
     5401 atccaccacg agcacgcgct ggccgttctg cgcaaagacg gtcgccaggt tgagaatggt
     5461 ggtcgacttg ccctcgcccg ccccgccgct gaccaccgtc agcgtgttcg ctccaggctc
     5521 cttgcgtgag aagaggatat tggtgcgcag cacgcggtaa gcctctccgt gcggactgtc
     5581 aggcccttca ttgaccagga tgcccacgtt ctgcggaatc acacccagga ccggcgcact
     5641 cagcgatcgc tcgacatcgt cgatggtctt gacgctcgta tcgaggtact cgatgaagaa
     5701 cgccaggccg acgcccatga ccaggcccac cataatgccc agggcgatgt tcaacgggat
     5761 attgggccga acaggtcgca gagcgggctc ggcgggatcg acgatccgca ccatcgattc
     5821 cttcggcaac tcggcatcca cttcctcctg cagcatgcgc atcaggatcg cgtctctgat
     5881 cttctgctta ttctcgagtt cgcgcttgct gctccaatac tcgcgggaac gcttggcttc
     5941 ctcgaccacc agcttcttgc cgtcgtccac tgccttctgg agttcatcgc ggcgagcctt
     6001 gagcgagatc acctggttct ccaaaccggt caggatgccc gtcagccgcg cctcgacctg
     6061 ctgatcgatt tgctcgacaa gcgcgagctt gcgcttcatt tcgggataat cgttcgaaaa
     6121 atcctctcgc agtcgggcca cttcctgctg tgccagagac aacgctccca aaagctcgcc
     6181 caaccgcgct tccgtgggca tcgtgaccag caccgcctcc ctcatggatg tccgatcgag
     6241 cttcttcaaa tcctcgagct ttgtgagaaa atacacgtac tccgagtgag cgcgcgtcag
     6301 ttcctgcgcc atggcgctga ctttctcgcg ttcgagcgat gtctgataga ccgaggcgtc
     6361 gttgatcgag tcgggaatat tcaactcggc ccgcagttga tccacgttgt cctgcaactt
     6421 cgcgacggcc tccgcctgct catcgagctt cgccctgagc ttggaaatgc cggcgctcgt
     6481 ttgccgctgt ttctcctcga cccgcgactc cttgtagaca ttggcgactt cgttcgcgat
     6541 ggccgaggcc tcgtcccggt tctcgctgta ggcgcgaatc tcgatgaggc tggtgtttct
     6601 cgattgacgc acatcgaggt tccgacgcag cagcaaatac gcctccggct cgctcacagc
     6661 gttcttgagt cgctgtcccc aggtcttgcg caagtccagc gactcgatca ccttgtagag
     6721 aatcggcttc gactggatct tctcgaactc cgtctggatg aagtaagggt cgaactgatt
     6781 ctgaacctgc ggacgcagga aagggtcgaa atccgtcggc tgcttctcga cctcgatgcg
     6841 ggccgtgctc atgaacgtgg gcttgagcac gaacgtcacc gccgtggtcg tcaggacgac
     6901 cagcagaaag acggccaaaa tgacggtctt gcggatgcga ataatccgcc agtaatcgag
     6961 gaaatgcagt tggctgtcag aaatcggatt tggcttggtt gcgtccatgt gtcaaaaaaa
     7021 aggaggccag aatcaatgct ccagcctccc acgttgtgtc gaatctgggg gtggtcagga
     7081 cctttagtag gtggctttta cgcccacgta gaaccgattg cgatcgtaat cgcgcatggg
     7141 gatgtcggaa tcgagcttgt cgtaggcgta gccgacctcg gccgcgaagt tggccgtgat
     7201 ctcgtaggcc agattgacgt cgacggtgta gaagttgtcg ctgtcgccgt catacatgcc
     7261 ttcctcgaaa gtcccatgct gccaggaacc tcgcaaggtc ccgataagtt tcgggaagat
     7321 ccgatgcgcc acaaacgcgt aaacggtcgt cgcttcctgt gaaacggtga acgaactcag
     7381 atcgtccgtt ctggcaatgg caacatcagt cgggcgcaga tcgtgtttga cacccacgga
     7441 cactttgctg ccctccatgt actcgtatgc cagagccgca tccacaaacg gcgccgtcac
     7501 gtcgtccaca tgcgcattcg gataccgggc gtaattgacg ccggcccgaa tctgcgcggt
     7561 cagacccggc agagccgtgt agtcaacccc cagaaatccg taatgcgact cctgattgcg
     7621 agcatcgctc tccggcaatg tccgatcggg agtgtcggtg taccagagct ggtcgccact
     7681 cgtataatcg aaatagccgt actgatagcc gaccaagccg atgatcgccg gttggaactg
     7741 ccaccgcagg tctgcactcg ccaagtgctc catacgatcc aacagggccg agatgctccc
     7801 cgccccgtcg tcttggtagt tgtagtacgt gttcgagtag cccagacgcg atcccagttg
     7861 ctccgtcaag ctggtcgtca gaccgatgcc ggcgtaattg cgccacgcgt tgccatcggt
     7921 gcgaacgtaa ctggcctgac cgccgttggg ttcgagaatg ctcggctcct cggaataaac
     7981 gggggattcg aagaaatcca accggaaccg ctcggaaaac gcatgcgagc ccgcgatctc
     8041 cgcccgcacg gtgtggtcaa attccttgct gctcggctgg gcctcgtacc agcgtaaatc
     8101 ataatccaaa ctcgccgtca gcgtggtctg gtcgcgcagc agcacgtact tgatgcccgg
     8161 ggcgaaattg atcccccaac tctccttgtc gacgttccca agatactcct tcgcctcagg
     8221 cgtcagattg gatggcgtcg ttcggtagtt gtcgtcgtag aagccgcgca acttggccga
     8281 tacgctccac ggccgcccgg acccgtcgac tgcctggccc tgcaggctga gtgcacccac
     8341 tgccgctaag ctcgcaaacg tgatcagttt attcataaag catcttgccc tgatttggat
     8401 agcgctgcct cgatccagtt caaatttagc ttgaaaggtt gagtgctttt taaggtttcg
     8461 actgagggtt gtcaaacata aatctctcag aatctgcgtt tccaggcttt tcggcgtgcc
     8521 cacgggcctg cgctccccac ccaacaacgt ccagaccacc gccttaaatc tcctgaaggc
     8581 acgatcaagg ctgagccagg gcggctctga gcaggtcgga ggccgacgcc gccaggcctc
     8641 atgacgcccc gagtgtcggg cgatgcgcgg cgcggctcgc taaccaataa acagccaacg
     8701 cgagccagat gatgccgaaa cccagggcgc gcgcgcgtgg gaacggttcc ccatacaccg
     8761 ccacccccag aatcaatccg caggtgggcg acacatattg gagcaagccc agcgtggaga
     8821 gtttcaccag gcgtgcagcc gacgcaaaga gcaggagggg cagggccgtc accacgcccg
     8881 tgagaaccaa caggctgtcg ccccaaccgc cgctctgtcc gaaacggccg atgccctggg
     8941 tctcgagtgc gccgagatac ccggcagccg gaagccagag cagcgcagtc tcgatcgtca
     9001 gccctggcag agcgggcagg ggagcggtct tcttgatcaa tccgtaacat gcgaagctca
     9061 gcgccagaac gaccgcaacc caggggaacg ccccgtactg ccaagtgagg aacagcaccc
     9121 ccagggtcgc gattccaacc gccatccact ggatggctcc cagcctttcc cgcagcacca
     9181 ccacgcccaa cgccacgctc acgagcggat tgatgtagta cccaagactc gtctccacga
     9241 tccgatcgtg gttcaccgcc caaatgtaga cgaaccaatt cagtcccaag agcagcgctg
     9301 cgccaaggta aatcgccaga gtccgccgct gcgttgccgc cgcaagcagc gtccgcccct
     9361 gtctcgacag gatgacgagc agggcgagaa agccgaacga ccagacgatg cggtgcatga
     9421 gaatctccgg agccggcacg gttcgcagcg ccttccagta caccgggaga agcccccaga
     9481 gcagataggc ccccagcccc agccagattc ctttgcgcgc ggacgacatc gccgagtgcg
     9541 cccttcaggg cgtgaacagg attcgattca cacctggcgg gtgcttcgct ggtgtcagtg
     9601 acggaaatgg cgaatccccg tgaagaccat cgccacgccc cgcgcatcag ccgccgcaat
     9661 gacctcggcg tcgcgcttcg agccgcccgg ctgaatcgcc gccgtcgccc ccgcctccgc
     9721 ggccgcaacc agcccatcgg ggaacggaaa gaacgcatcg ctgcagacca cgctgccccg
     9781 caaagacaac ccggcctcgc ccgccttcca gacggcgatg cgactggcat ccacccggct
     9841 catctgaccc gccccgatcc ccagcgtgcg atccgccgcg gcatacacga tcgcgttcga
     9901 cttgacatgc ttcaccaccc gccacccgaa caacaaggcc tgatactcat cctcgctggg
     9961 cgcccgcttc gtcacgacct tgacctggtc ccgcgctcgc accgtcgcgc ggtccggctg
    10021 ctgccaaaga aacgactcga agccaacgcc ccgaatctcc ccccccgcgg tggcgtgcga
    10081 gtccatcgac acctcgatca gccgcaggtt cctcttctgc tgcagcaccg ccaccgcctc
    10141 cggcgtaaag gagggggcca cgaccacctc gctgaagatc tcggcaatgg ccgtcgcaca
    10201 ggggccgtcc atcggttgat tcacagcgat gatgccgccg aagggcgcct gccgatccgt
    10261 ggccagcgcc ttctcccacg cctcgcacag cgacgctccc cggccgaccc cgcacggatt
    10321 ggtatgcttg agaatggcca gcgtcggagc atcgccccga aactccgaga tcaaggtcgc
    10381 cgccgacgtc agatcgagaa tgttgttgta ggaaagttcc ttgccatgaa tctgccggaa
    10441 gtgatcggca aaccgcccgt agagagccgc cggctgatgc ggattctccc cgtaccgcag
    10501 cgcttgcacc cgcggcgcat ccacgatcag ccggtccgga ggcgcgctgc cacccaaccg
    10561 cccgaccgcc gccagatcgg ttccgcctcc cgcaccgaac cagtgcggca ggtgggcggc
    10621 aatcgccccg tcgtacgccg cggtgcgcgc atacaccttc acagcgagcc ggcgacgcaa
    10681 ctccaacgtg gtgccgcccc gcgcttgcac ctgggccgca acctcttcgt aatccgcagg
    10741 atcgacgatc gccgtgacgt gttcgtggtt cttggccgcg ctccgcagca tggccgggcc
    10801 gccgatgtcg atgttctcca ccacgtccgc ccgagtcaca ccgggccgga cgaccgtctc
    10861 ctcgaacggg tagaggttca ccgccaccag atcgatcggg cggatgccgt gacgatccgc
    10921 cgtcgcttcg tgctccgggt ggccgcgcag atacagcagc ccgccatgaa ccttcggatg
    10981 aagagtcttg acccggccgt cgagcatctc cgggaacccg gtgaactcgc ccaactcgct
    11041 caccggcaaa cccgcctccc gcagcgcccg agcggtgccg ccggtcgaca acagctcgac
    11101 accggcaccg accagcaccc gcgcgaaggg cagcaggccg gttttgtcgt acacggagag
    11161 aagtgcgcgt cggatcggtt gcataggcaa agggcaaaat gccgcccttg ccaaagccga
    11221 gtcaatggac aaatggggct aaagcagccg gggctgcaaa tcctcacgcg caagaagctc
    11281 cccgagactc tgcacgcgcg cgcgccgccg ttggatgatt tgcttgtcga gcgccaggtc
    11341 gtagcggacc gggtagtccc cgtcatagca ggccaggcag aaggccgagg ctggcaggcc
    11401 ggtcgcccgc accattcccg cctgcgagag ataggccagc gaatcggcct tcaagtaccg
    11461 gcatatctcg tcgacgctgt gattggcggc catcagcttg ctgcggtccg ggaaatcgat
    11521 cccgtagacg cacgggttga cgtgcggcgg acaactcacc atcacatgca cttcccgtgc
    11581 ccccgcttcc ttcaggttgg caacccgctg ccggcaggtc gtgccgcgca cgatcgaatc
    11641 gtcgacgatg atcacgcgct tgccggccac cacttcgcgg atcaggttta gcttcacccg
    11701 cacattgaaa tcgcggatga gctgcgaagg ctggaggaaa ctccgcccca cataatggtt
    11761 gcgaacaaac gccatctcga agggaatccc cgattcgagc gaatagccca gcgcggcgca
    11821 attgccgctg tccggcaccg gcacgaccag gtcggcctgg cgcggatact cgcgagccaa
    11881 ttcccggccc atcgccacgc gcactctgtg cacattacgt ccggcgatcg tgctgtccgg
    11941 ccgcgcgaag tagacgtact cgaaaatgca gaaggcgcgc cgcgggtgat cgggaaacgc
    12001 ctggatgctc cgcaggccct ccctgttaat caccagaatc tcgcccggag cgatgtcacg
    12061 cacaaacgtg gcctggatca gatcgaacgc gcaggtctcg ctcgacaaca cccaggcgtc
    12121 ccccagccgc ccgagcgaca gaggccggaa tccatgcgga tcacgaaccc cgatcagctc
    12181 ctgctccgtc tgcaggatca gcgagtaggc gccctcgatt cgacgcaatg tctggaccag
    12241 gctggaggca gcccccctcg ggccggggcg ggccatcagg tgcaggatga tctcactgtc
    12301 cacagtggtc tggaaaagcg tgccttgcgc ttcgagttcc tcgcgaagct gaacggcgtt
    12361 ggtcagattc ccgttgtgcg cgatcgcgat ctgtccgcgc gccgaatcgg cggtgagggg
    12421 ttgcgcattc cggatatggg acgaacccat cgtcgagtag cgggtgtgcc ccatggccat
    12481 gtccccctcg agatcgcgca gaatgcgccc gctgaagacc tgcggcacga gccccatccc
    12541 gcggtggacc cggaactggc gtccgtcgca cgagacgatg ccggcgctct cttgcccgcg
    12601 gtgctgcaag gcgtagaggc cgtagtacgt gagctccgcg gcattcggat gcccatagac
    12661 gccgaacaca ccgcaatggt ctttgggatg ctgctgcata gaaccaggct gtcgatccac
    12721 cggcgcggcc attccagccc gccgcactcc cggctgcaag cttcaagtgc gcctcagagc
    12781 cgcatgcctc agacaatgcc tcagagcagc attgccaaag ccgcacccgg ccgctacttt
    12841 ccgcgttcgc gttgaggtaa agacccggcg gggcgagact gctgtcggcc cggttcccaa
    12901 cctccggtgc ggccatggcc gaatacaaag tccagtttga agtcttcgaa ggaccgctcg
    12961 acctcctgct ctatctcgtc aggaaggagg aggtcgacat ctaccaggta aacctcaccc
    13021 gcctggccac ccagttcctc gagtatgtcg acctgatgcg ccaactcgac ctggacattg
    13081 cgggcgagtt tctggtgatg gcgtcgacgc tcatgtatct caagagccgc gaacttctcc
    13141 ccgtcgacca gcaggccatc tccctcgacg aggacgagga cagcgacgac ccgcgctggg
    13201 aactgattcg caagctggtc gagtacagaa agttcaagga cgccgcctcc cagctgcagg
    13261 cgctcgagtt gcagcaggag caaatctacc cgcgccagcc cgccaaaccg gaattcgagc
    13321 ccgttcccgc tcgcatcgag gtctccctct tcgacctcat cggcgcggtc agcgcgatca
    13381 tgaagcgcat tcaggaacgc gaagagaccc gcgacatctt cgaggacaag tggatcgtca
    13441 gtgagagaat cgacttcatc cgcacactcc tgggttcccg ccccgccttc acctttgccg
    13501 atctgttcgg acaggtgacc agccgttcgg aggtcgtcgc cacattcctc gccctcctcg
    13561 aactgatccg cttgcaccat gccgtcgctt tccagcgcga accctttggc gagatcgaaa
    13621 ttgccgccgc gccaactcct ccgcccggcc cggcccctgg cccttcgctc gactccgacc
    13681 ccgctgccgg cgtgtcggac acccatgcgg cagtctgccc cgacgacagc gcgccccccc
    13741 cgtttgcatg atcgagttga agtccattct cgaagccgtt ctcttctccg cgcaaaaacc
    13801 gctgcgcgcc ctcgagttga agaacatcct gaccgccgcc gccgagcagt ccgacgacga
    13861 ggccgcccgc gcccagaagc gcgtcaagac cgcggacatc gattccgcgc tcgagcagct
    13921 tcgcgaagag cacgccgccg ccgaccgcag cttcagcctg gcctgcatcg ccgacggctg
    13981 gcaattcgtc agcaagcccg cctatgcgcc ctggctgcgc gtgctcgccg gcgaacgcgc
    14041 ccgccccgcc cgcctctccg cgcccgcgct cgagaccctc acgatcatcg cctatcgcca
    14101 gccggtcacc cgcgccgaaa tcgagcaaat ccgcggcgtc tcgatcgatg gcgtcatgca
    14161 gaccctcctg gaacggaacc tgatcgaggc cgtggggcgc gccgaggttg tcgggcggcc
    14221 catgcagtac ggaacaacac gcctgttcct cgagtatttc gggctccgat cgctcgacga
    14281 catgcccacc gccgatgagc tgcgacgcat ccccgtcgac cgtccgccca gcctcctcac
    14341 cgtcgaaccc ggcctggcca cagcgcctcc cgagcaactg acactgcaac cccaagagca
    14401 atcagagcca tgccctcacg ccacgtcgca aaatccggcc gcagagccct cgccggccct
    14461 cgatcgtcaa cccgcgcacg acgccccatc cgagcccgtt ccggcgccgg gcggggagaa
    14521 tctgcagtcc ccgcttcaag aatgagcctt ctcgaacatc gcaccgccat tgaccgcacc
    14581 gaccaacaga ttgttcggtt gctcaaccaa cgcacgcgcc acgtgctggc catcggctcg
    14641 atcaaactga aaagcggcca ggaaatctac gcgccccatc gcgagcggac cgtgctcgat
    14701 cggatcgcca aaatgaaccg cggccccctg agctcggagg ccattcgcgc catctaccgc
    14761 gagatcatgt ccagcgccct cgccgtcgag aagccgatga ccatcgcata cctggggccg
    14821 gaggcgacat tcacccacca ggcggccatc caacggttcg gcaccagcct gcgctacgcg
    14881 gcgctcaaga ctatcgccga cgtcttcagc gaagtggcca agcgacacgc ggatttcggc
    14941 gtcgtcccca tcgagaactc gaccgaaggc gtggtcaccc acacgctcga catgttcatg
    15001 gacagcgacc tgaagatcgt cgcccagatc gtcatcccca ttcagcactg cctcatgggc
    15061 cgggcccgcc gacagcagat tcgccgcctt ttcgtccacc cccagtcgct cgcccagtgc
    15121 cgcggctggc tccagcgcaa cctccccaac gccgagatca tcgagacctc ctcgaatgcg
    15181 cgctcggccg agctggccgc aggcgccagg aacgccgtcg ccatcgccgg cctgctcgcc
    15241 gccgagaaat acggccttcc cgtcatcgaa cataacatcc aggacaacgc cgccaacgca
    15301 acccgctttc tagtcctcgg acgtcaatcc ccgcctcgtt cgggacgcga tcgcaccagc
    15361 ctcatgttct gcatcgccga ccgcgtcggc gccctccacg gcgccatcgc tcctttccgc
    15421 aaataccgca tcaacatgac caaaatcgag tcccgtccca gcaagcgcaa ggcatgggag
    15481 tactatttct tcgtcgactg cgacggccac atcgaggatc cgcgtgtcgc caagatgctc
    15541 gaagcactcg gacgacactg cgtctttgtc aaagtgctcg gttcgtaccc gaacaccgat
    15601 tagtccgggg ccaaaacccc cggccccggt ccatcaccca aaaaactcga tctcccaaac
    15661 cttgaatgtc tcagccaaaa caacccgcac aaaccgactc ctatgcaatc ctctctcccc
    15721 tccgtatcga tcctgctcgc ggccctcaca gcctccaccc tcatcggtca agacgtgaaa
    15781 cctgattccc tccagcaggc acgcagcttc caacggtccg tcactcgcac cgtcggggcg
    15841 gactacctgt tgttttttcc ccgtgggtat gacgaggccg tggaaaagcg gtggccgctg
    15901 atcctgttcc tccacggagc cggcgagcgc ggcaccaaca tctggctcgt cgccaagcac
    15961 ggtcctccca agctcgtgac tgccaagccg gacatgccgt tcattattgc ttcgccccag
    16021 tgtccaccgg gccaaacgtg gtcgaacgac accgtgctgg cactgcttga cgaattgctg
    16081 gcaaatcacc gtgtcgatcc cgagcgcgtc tacctcaccg gcttgagcat gggcgggtat
    16141 ggaacctgga gcctggcgct ggccgctccg gagcgtttcg ccgccgtcgt gcccatctgc
    16201 ggcggaggcg atccgatcaa gctgctcctc gccgatcccc gacgggcgca ggctctcaag
    16261 agcttgccga tctgggcctt ccatggcgcc aaagacccag tggtcaaact cgaggaatcg
    16321 gagcgcatgg tggacgcgct caagcgattc ggttgcagcg aggttcaact gacagtctat
    16381 ccggaagccg gccacgatgc ctggacccag acctatgaga atcccaaact ctacgactgg
    16441 ctccttgccc atcgccgcac tgcatcgtcg aggtgactgg agggtgaccg tcctcggccg
    16501 cagcaatgac gaaatgcacc atgagattca taaggtggac gcgcaagcgc acccgcagga
    16561 gaggccctga ctgatgacga tgattgtctt gagatcagcc ggagccgggc gggcgctacg
    16621 gcaacacggc aatgcgactc cgaatcgccc gcaccaactc tgccggatcg gcctcgttta
    16681 gccgccgcag cacgcattcc caacgatagg agaaattgcc ctcgggatcc ggcggcatgt
    16741 gcttgaacag cgcatcaaag gcccgccgat ggtcacggta atgcccctgg atgtgccgga
    16801 accatacgtc gaatccctcc aaataggcca aggcaaactc gcggggatgg gccagcttgt
    16861 cggaccggtc gttgaccggc ttggcatacg cgggcgcaag ttccagcaac gtctgctgat
    16921 agcagacgaa tgatcctgtg tggtcgcaca caatcagttc cttgggcatc ccctgttcgt
    16981 cctcgaccac gatctcgtcg ccgacatcga acaccacctg ctgggacatg gggtcgccgc
    17041 gtcccacaat gagattggcg gctgccgctt tcccgaacag gcgcgcgaac gggtttgcaa
    17101 actcgggatt ctcgtagcgc gaccggggga gctggttggc ggggaacccc gagatgaaat
    17161 cgcgctcgaa gtaggtcacc ggcaggcgca ggcccgccga ggccgcggcg ctgccgtcgt
    17221 agggttcctg gaggcgccgc atgaccatgc acaaaggcac gttgacgccc aaataccagc
    17281 agccgagccg gcgattcagg atgtactcga tgtaatcctc ggt
//
LOCUS       DAOQRO010000019        17099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2449_length_17099_cov_5.240319, whole genome shotgun sequence.
ACCESSION   DAOQRO010000019 DAOQRO010000000
VERSION     DAOQRO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_17099_cov_5.240319"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKK20_01495"
     CDS             <1..308
                     /locus_tag="PKK20_01495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU98596.1"
                     /translation="RGLAEMWRVAKPGGRLVILDFGKPANRWWHGVYYAYLRHLVPVF
                     GRLACGDAAAYAYILESLERYAAQEGVSAMLRELGAMDVRVINLIGGAMSIHCAVRH"
     gene            634..2481
                     /locus_tag="PKK20_01500"
     CDS             634..2481
                     /locus_tag="PKK20_01500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNU98597.1"
                     /translation="MRAAHRVLRAPLAALVSPGSGLAGFILGLSLAVQSSGAASGTNS
                     SIQDTVRGIALRLSQRHSAEELNAFSTVRLLEELTPAEQRVLGGEHIRFEVDVPVVVT
                     VLRDTAGDEPFWLRSRRFTPTGAILSVAKRRFEAWERLFPSGAIGLGVNSLAGSGEHY
                     LVLVRAQQDGAPLRIDHLDPERLRVTEMAPGARPYADRDETLDEIPEAWRGWRLIQTL
                     RQSRDEARLIGGFRETSHPSSRRPDQIVLTWSGDPRTTQAIQWRTAPSVGSGWVAYGK
                     RAELNTVRPRRLRKVRAVTDTLVDGRLANDPIVHRHTAELRDLEPGTAYVYCVGDGSA
                     DGWSELAEFSTAPDGSSGFSFVYMGDPQNGFERWGTLARTAFRQRPDAAFYLLAGDLV
                     NRGAERDDWDGLFANAAGILNRRPVVPALGNHDCQGGHPTLYLKQFALPRNGPPGLEA
                     ERAYAFHYANALLVVLDSNSEPGAQTAWLEAQLEGSKAKWKFVAYHHPAYSSAPNRDN
                     VALREAWTPLFDRYQVDLALQGHDHAYLRTYPLKAGQRGSDAGDGTHYLVSVSGTKMY
                     RQDARPYTEVGLTNVSTFQVIDIQRQGDRLVYRAYDVDGDLRDQLVIEK"
     gene            3126..3893
                     /locus_tag="PKK20_01505"
     CDS             3126..3893
                     /locus_tag="PKK20_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU98598.1"
                     /translation="MMLGFITSFLDWRNPLFLLFGLFALWMLVDAIRRQEWLWAVFIF
                     LFPPINAVLYFFLVYRQAAPAMRGFELSGAQDRHRIKELQEQIHHLDTAHHHAQLGDI
                     YSRQGKLDQAEACYRAAIQRDPEDLDTLAHYGQCLLRQNRAAEALPFFERVCGQDADH
                     DYGQSLMALAETYGALGRAEEAIQSWRRVLASHTYARARVKLAKLLMASGQVERARME
                     LDEVLADDPHAPAFQRKRDAVWIREARAMQRRLLGRA"
     gene            3982..4668
                     /gene="rpe"
                     /locus_tag="PKK20_01510"
     CDS             3982..4668
                     /gene="rpe"
                     /locus_tag="PKK20_01510"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNU98599.1"
                     /translation="MIIAPSLLAADFGRLGSEIQRADRAGADWIHVDIMDGHFVPNIS
                     FGPGVVETARRFTRLPLDVHLMCSRPATLLEPFVRAGASLITVHVELGNGVGDLLWKI
                     RALGRKVGLAVNPPTPIANALPLLKQIDLLLIMTVNPGFGGQSFIEETIPKVQQADAW
                     RRERGLPFRIEVDGGINLSTAAECARAGADTFVSGTALFGQSNLKAAIRKMREAAMRH
                     APAPRGGART"
     gene            4907..5914
                     /locus_tag="PKK20_01515"
     CDS             4907..5914
                     /locus_tag="PKK20_01515"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNU98600.1"
                     /translation="MHAQPHVAVVGATGAVGVEMMKSLERRRFPLRELTLLASARSVG
                     KTFEFQGKTIAVKELKPDSFAGVDIALFSAGGSVSREFAPVAARAGCVVIDNSSAFRM
                     DDSVPLVVPEINASDVRSHKGIIANPNCTTAIALMAIYPLHKAFFCKRIFASSYQAVS
                     GTGAKAIEELRRQVDQIVHGQPVTKEVYPHQIAFNVLPHIDSFLPSGYTKEEMKMENE
                     GRKIMHHPTFRASVTCVRVPVYRAHSIAVSAEFEKPVTLDAARAALAQAPGLDVVDDP
                     ANKVYPMPLFTAEKDNCEVGRLRLDCALDNGLCFWVSGDQLVKGAALNAVQIAEELLK
                     P"
     gene            6112..6342
                     /locus_tag="PKK20_01520"
                     /pseudo
     CDS             6112..6342
                     /locus_tag="PKK20_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            6411..7007
                     /locus_tag="PKK20_01525"
     CDS             6411..7007
                     /locus_tag="PKK20_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98601.1"
                     /translation="MLGEEHYKFTSPPSPNPAFTNLNYLNDPQHAAFAQYIPSVATYR
                     CPEDRTTVELNGRAHPKVRTYALNSYLGWAEWVPPGYNSTRHWTFEKWSALAEASPTT
                     IFSFIDVAPGNVCLPAFVVRMGAGGQFYHLPSIQHGGRGAVAFGDGHVETRRWIESRT
                     LTEAALPWNPNHWTLWDPGNRDLEWLQDHATVPRPSAE"
     gene            complement(7340..8278)
                     /locus_tag="PKK20_01530"
     CDS             complement(7340..8278)
                     /locus_tag="PKK20_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNU98602.1"
                     /translation="MNDLLDIPADPEPSSSEVQHLFVDEAGTPTLFHESGKPIADTFG
                     CSRFFILGKLEVENPPVVATALTELRQRMLADPIFAGTESFKPERKKTAIAFHAKDDL
                     PEVRVPVFELLRSFGSQLRFYAVVCDKLKLTETESAKRAKEPGYRFNENSVYDALMRE
                     LFGKFHRLADRYEVNVARRGTSTRNEAIRVALAHAEQDFERKFGFRRSSPDAWKITVF
                     RSKEAPCLQAVDYFLWALQRFYEVKWDVKTRKKALDPTTGLVIHEDRFLRAMWPQVGE
                     VHDLHFARAHGTFFTAREPLTLDRRFPPPKGKKKKS"
     gene            complement(8286..11759)
                     /locus_tag="PKK20_01535"
     CDS             complement(8286..11759)
                     /locus_tag="PKK20_01535"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNU98603.1"
                     /translation="MAPRDRLRSLRNFADLIRFLEEELAWPLQQYGFEELTFAYEAEE
                     LGLREEDAAKVKRIHQLRPLASGQPWGIFFIEFEKKRLPVVVLRRILSLLVIKKRGSA
                     NKSERAAWQPEDLLFVSAFGDEATDQREIAFAHFHQESGDLPTLRVLGWDGRDTPLKL
                     EHVDATLHDKLRWPADPRDLSSWRSQWTRAFRHRVGHVIRTADGLAERLAALARGICA
                     AAQTMMAHETERGALTKLYKAFQTALIHDLTPESFADTYAQTITYGLLTAAISRTEMS
                     AGTAGTFVRAEDVSDIVPVTNPFLKEMLQTFLKAGGRKGGIDFDELGIQDVVELLRGD
                     ETDLPAILRDFGNKNPDEDPVIHFYEHFLSAYNKKLKVQRGVFYTPPPVVSYIVRSVH
                     ELLQTEFGLAAGLASTVTWGEMVQRNPELKLPPRTDEPGEMRTIDPGEFFVQILDIAT
                     GTATFIVEVIDVVHRHLKTQWNKGGLAAMPRIPSPGGAGEAGAPAPGEGPGAQPSTFN
                     QYWNVYVPACLLPRLYGFELMMAPYAIAHMKVGLKLYETGYRFGSAERVRIYLTNSLE
                     PKVKQLPQIGFDALAHEAQAVNEIKWYKRFTVVVGNPPYAGISSNMTDHAQRLIDAYK
                     VVDGVALSERKLWLQDDYVKFIRLAQTIIDAAGVGVLGYVTNHGYLDNPTFRGMRQSL
                     VSTFARLRLLDLHGNANKKELAPDGLDKNVFDIRQGVAVCLATRSLAESSVQHAALWG
                     LREAKYAWLTNHDVDNTAFQRLSPNSPFYLFIPQNSDCRAEYERGYSLAEVFPKNGVG
                     IVTARDALTIDVDAGRLWQRVVRFCRLAPEDARAEYELGSDVQSWRVCWAQEDVRKSG
                     PDRSLVREVLYRPFDVRHVYYTGVSSGFVCRPVWDVMRCMVGGKNVAMVTTRTVEIGR
                     GFEHVFCTRGFIQHHSVSVKEVNYLFPLMLPPDDSQAGSLLREHHAPQVNLSSRFVRS
                     LADALNLVQRASDGLPSGISAEDIFHYAYAVFHSPGYRSRYAEFLKIDFPRLPLTSSL
                     ELFRALAHLGGELVALHLLESPTLEKPITKFIGSASPVVDKVTYEAGTVWFGKAKTCG
                     FRGVPEPVWNFHIGGYQVCEKWLKDRKGHVLSADDITHYHRIVVALSETIRLMGEVDA
                     IIAQHGGWPLK"
     gene            complement(11763..12374)
                     /locus_tag="PKK20_01540"
     CDS             complement(11763..12374)
                     /locus_tag="PKK20_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNU98604.1"
                     /translation="MIQPIVEGVGEIEAVPVLLRRLAAQMDVAHVPLGRPIRRGRGHL
                     IQREGLAKAIDVARRQTGCRGILVLFDADDLCPKTGAIPLQQEAARIAAAIPCPVVLA
                     NKEFEAWFLASLEDLSDRPAGRASYPGDPDQKRGAKEELERRLAMYYHAPADQPKFAA
                     RINLRRVHERSRSFRKLVKEYRLLLLALGFAPDQWPAIGPEAS"
     gene            complement(12371..13615)
                     /locus_tag="PKK20_01545"
     CDS             complement(12371..13615)
                     /locus_tag="PKK20_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU98605.1"
                     /translation="MRSEFVPIRKLWLKRFRSILAARLGFENPLFLVGKNGAGKSNVL
                     KALEFLSECMTTPLGAVFNNQGGIEAVRHRSGTKSRPPNMAVRVDFDFRNGGRSAGWY
                     AFEIRAKEGYEFDVVREKCVVQTGGQLSWFDRFHGSVTTSVPSIEPAVNPQALLLPLM
                     GGTAQFAPVTRGLSGIRVFSIQPKAILELQDPDSGSILKADGSNLASVLKSLSKDEEK
                     MERLCELLCTVVPGTATVRPIKHGKKLSLEFRQEWSENGGPRGKSRRAASKRTVAHGA
                     FSMSDGTLRTLGILTAFLQPNRSTLVGLEEPESTIHPEALSSILEMIRSFSRDTQVLV
                     TTHSPELLDAKWIEPENLRVVSWDKGMTQVLPLGEASVAALRDHLMGAGELLRSNALH
                     PVDFFADAQDPNQMELFADATP"
     gene            complement(13647..16847)
                     /locus_tag="PKK20_01550"
     CDS             complement(13647..16847)
                     /locus_tag="PKK20_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNU98606.1"
                     /translation="MIFPRPSGGELFIVDNSDESWKGLRYLHDWTEIASAFDIATGFF
                     EIGSLLALDGRWQKLDKIRILMGDQATARTRQAILEGLRQQVCQKLDASLEGEKEAND
                     LLTGAAAIVEAMREGRIQCRVYARRKFHAKAYITRPRVAVIGSVALVGSSNFTVPGLT
                     QNVELNIQIRAPGDVSQLQTWYEQHWQEAEEITPDVIKVIARHIAEYAPFEVYARALQ
                     GLFEHHTPSEAEWENGESKIYPVLDHYQKEGYHSLVNIARQHNGAFLCDGVGLGKTFV
                     GLMLIERLVMCDKKRVMLLVPKSGRVAVWERNIRRYLPHLLNGFLPFKIYNHTDLMRG
                     PSAGGRDFPSEFQQIKEQADVVVIDEAHHFRNRGLANAENGTIRSRYWQLYDIVGKKT
                     LFLLTATPVNNALTDFQHQIELFSRVDNPAAFAGALGIHNLASHFQQLEKAMKRLVAG
                     QEHGELFQVNQAEAEKVLFDDQLFRALVVQRSRAYAQQSQIQHGGTEAVFPAKEPPRV
                     AAYSVKKTYGHLLGKVEAAFSKPEQLFSLAVYYPLAKWRGDPAALNVFDENRQKQVVR
                     LIRIQFLKRFESSIYAFETSCQNLLLKLLAFLRRNSMAQSETKRLQRWEAQNGELLDH
                     VKQRRAEFQEEDESEEVGASELGDEFLDDFEVLDRNNYDLPEIFDETYADLEQLVDFL
                     EELKTFDASHDNKLQSLIKLLKSDAVLKKHKVLIFSEFMTTARYVRRELQGAGIEGVD
                     EIDSGSKTDRGDAIQRFAPYYNGTTSAELAAAGQKETRILISTDVLSEGLNLQDATRL
                     INYDLHWNPVRLMQRIGRVDRRLNPEIEARIVADHPDHAPLRGKVVYWNFLPPDELDD
                     LLRLYHRVSNKTLRISKVFGIEGKKLLKPEDDYEALRDFTHAYQGALTPLEQMHLEFQ
                     KLLADNPGLQHRLDQLPGRVFSGKEHTKPGAKALFLCYALPAEDKALRSDTAAAATGG
                     GAGQAEGAAAWTTGTGKAGWYLYDLATGKILEQPEEIVAFIRSTPETPRRVAMPQADL
                     HEVRLKVEKHIKNTYLKQVQAPVGVNPVLQCWMELN"
BASE COUNT         3105 a         5031 c         5557 g         3406 t
ORIGIN      
        1 agcgcgggct ggccgagatg tggcgggtgg ccaagccggg ggggcggctg gtcatcctgg
       61 atttcgggaa gccggcgaac cggtggtggc acggtgtcta ctacgcatac ctgcgccacc
      121 tggtgccggt cttcgggcgt ctggcttgcg gggacgctgc ggcgtacgca tacatactgg
      181 aatcgctcga acggtatgcc gcacaggagg gggtgagcgc gatgctgcgg gagttgggcg
      241 caatggacgt gcgggtgata aacctgattg gaggggcgat gagcattcat tgcgcggtgc
      301 gccactagcc gcatgttcaa tagagttgtg accgccgcga agcggaatac cccacaatgc
      361 ggaccactgc ggggcagccg gtggaattcg tattcgacac gctgcgtcgc cttcgatttc
      421 aatggcacgc acatgccaac cgattcgttg ccgataaatc ctcgccttcg cacggctgac
      481 cgctctcgga gggccgagct ccgcgaggcc ccaactgtcg accattaccg tttcgagcca
      541 aggactctcc cgagcctgcg catacgggat cggccctccg gcagggattc gtggtccccg
      601 tgcgcatctg gcgaaggaat gtcatcgctc tccatgagag cagcgcatcg ggttctgcgt
      661 gcgccgcttg ccgccttggt cagtccgggc tcggggctgg cggggttcat cctggggttg
      721 agtcttgccg tccagtcaag tggggcggcg tctgggacga attcgtcgat tcaggacacg
      781 gtgagaggga tcgccttgcg cctgagccag cggcattctg cggaggagct caacgcgttc
      841 agcacggtgc gtctgctcga ggagttgacg ccggcagaac agcgcgtttt gggcggggaa
      901 catattcggt tcgaggtcga tgtgccggtg gtcgtaaccg tgctgcgcga taccgccgga
      961 gacgagccgt tttggctgcg ctctcgcaga ttcacgccga cgggtgcgat cctcagcgtg
     1021 gcgaagcggc ggttcgaggc ctgggagcgg ttgttcccgt ccggagcgat tggtttggga
     1081 gtcaactcac tggccggcag cggcgagcat tacctggtgc tggtgcgagc gcagcaagat
     1141 ggggcgccgc tgcggatcga ccacctcgat ccggagcgtt tgcgggtcac agagatggcg
     1201 ccgggggcga ggccgtacgc cgatcgcgac gaaaccctcg acgagattcc cgaggcttgg
     1261 cggggttggc ggctcatcca gacactgcgg cagagtcggg acgaggcccg gctcatcggg
     1321 ggctttcggg agacgtcaca tcccagctcg aggcggccgg accagatcgt gctgacatgg
     1381 agtggggatc cgcgaacgac ccaggcgatt cagtggcgga ccgctccgtc ggtcgggtcc
     1441 ggctgggtgg cgtatggcaa gcgcgccgag ttgaacaccg tcaggccgcg ccggctgcgc
     1501 aaggtgcggg cggtcacgga cacgctggtg gacgggcgat tggcgaacga cccgattgtg
     1561 catcggcaca ccgcggagct gagggatctg gaaccgggca cggcttacgt ctattgcgtg
     1621 ggtgacggtt cggcggacgg gtggtccgag ctggccgagt tcagcacggc gccggacggg
     1681 tcgtccgggt tcagttttgt ctacatgggg gatccgcaaa acggtttcga acgctgggga
     1741 acgctcgcgc gcacggcatt ccggcagcgg ccggatgcgg cgttctatct gttggcaggc
     1801 gacctggtga accgcggggc ggaacgggac gactgggacg gcctgttcgc caatgccgcg
     1861 ggcatcttga accggcgccc cgtggtgcca gcgcttggga accacgattg tcagggaggc
     1921 catccgacgc tttacctgaa gcagttcgcg ctgccgcgca acgggccgcc cgggctcgaa
     1981 gcggagcggg cctatgcgtt tcattacgcc aatgctttgc tggtcgtgct ggactcgaac
     2041 tcggagcccg gcgcgcagac cgcgtggctc gaggcgcaac tcgaggggag caaggccaag
     2101 tggaagtttg tcgcctatca tcatcccgcc tattcgtccg cgcccaatcg cgacaacgtt
     2161 gcattgcggg aggcgtggac tccgttgttc gaccggtacc aggtcgacct ggctcttcaa
     2221 ggacatgacc atgcctattt gcggacgtat ccgctgaagg caggccagcg cggctcggat
     2281 gccggggatg ggacgcacta tctcgtctcg gtctcgggga ccaagatgta ccgccaggac
     2341 gcgcgtccgt acacggaggt tggactgacc aacgtgtcca cgttccaggt gatcgacatc
     2401 cagaggcagg gcgaccggct ggtctatcgg gcctacgacg tggacggcga cctgcgcgac
     2461 cagttggtca tcgagaagta ggcggcgagt ccggcgccca ccagacatcg aggcgccgtt
     2521 gaagctccgt ggaacatccg gactaagggt ctgagcaaaa attacttccg attttggcgg
     2581 gagcgccacc gggccagatg caaggcccga gggagggagt gtatcccttg agatactcga
     2641 ccgaccgagg aacgacgcag atggtccggt gccgccgcgc cccgaagggc tggggggctt
     2701 tggctcgatg gtcccgcgga cgccggattg ctcctcgctc acagaccggt gcgtttatgc
     2761 tccctcgtcg cgcctggcca gcgagccaaa tcccctccag caaaaccgga attgattttt
     2821 gcacagaccc taaaggaagt tggcaggcgg cgctcactgg tctaaggtga ggccgatgtg
     2881 agccaatcgg cgcgaatact cgaagcgaac ggacaagacg aactcggcga gtctgtcggt
     2941 tggccggtgt tcgccctaag cagtctggaa tcctcgattg gtgaaactcg ttgtcgtccc
     3001 atcaagttct tgcgcagcgc agcagcgcca ctgagccaga cgtcgagttt tgcagacggt
     3061 cgacgaagtc cagcgcgcac gaatgggtca cacgcctcgt gggtccgggg cagagccggg
     3121 ctaacatgat gctcggcttc atcaccagct tcctggactg gcgcaacccg ctgttcctgc
     3181 tctttgggct gttcgcttta tggatgctgg tggacgcgat tcgccgccag gaatggctct
     3241 gggcggtgtt catctttctt tttcccccga tcaacgcagt gctttacttc tttctggtgt
     3301 atcggcaggc cgccccggcg atgaggggat tcgaactgtc gggagcgcag gatcgccatc
     3361 ggatcaagga attgcaggag caaatccatc atctcgacac ggctcatcac cacgcgcaac
     3421 ttggagacat ctattcccgg cagggcaagc tcgaccaggc ggaggcgtgc tatcgcgccg
     3481 cgattcagag ggatccggag gatttggaca cgctggcgca ctacgggcag tgtttgctcc
     3541 ggcagaaccg cgcggcggag gctctgccct ttttcgagag ggtttgcggg caggatgccg
     3601 accacgacta tggccaatcg ctgatggcgt tggcggagac ttacggcgcc ctcggtcgcg
     3661 ccgaggaagc gatccaatcc tggcgccgag tgctcgccag ccacacctat gcccgggccc
     3721 gagtgaaact cgcaaagctc ctcatggcct cgggccaggt cgagcgggcg cgcatggaac
     3781 tcgatgaagt gcttgcggac gatccgcatg cccccgcatt ccagcgaaaa cgggatgcgg
     3841 tctggattcg cgaggccaga gcgatgcagc gccgtctcct gggcagggcc tgaatcggcg
     3901 agttgagagt tgagagttga aagtcacgga ctcgcggcac gcacttgcgg agaggcctcg
     3961 tggtccgcta gcttgtggcc gatgattatt gcaccgtctc tgctcgcagc tgattttggt
     4021 cggctgggat cggagattca acgcgccgat cgagccggag cggattggat ccatgtggac
     4081 atcatggatg ggcatttcgt gccgaacatt tcattcgggc ccggcgtggt ggagacggcg
     4141 cgtcgtttca cgcggctgcc gttggacgtg cacctgatgt gttcgcgtcc ggccactctg
     4201 ctcgaaccgt tcgtccgggc gggggcgtcg ctgatcaccg tgcacgtcga gttggggaac
     4261 ggggtcggcg atctgctgtg gaagattcgg gccctcggac ggaaggtcgg cctggccgtc
     4321 aacccgccca cgcccatcgc caatgccctg ccgctgctca agcaaattga tctgctcctt
     4381 attatgacgg tgaatccggg cttcggcggg cagtcgttca tcgaggagac aatcccgaag
     4441 gtccaacagg cggacgcatg gcgccgcgag cggggtctgc ccttccgcat cgaggtcgac
     4501 ggcgggatca acctgtcgac cgcggcggaa tgcgcgcggg ctggagcgga cacgtttgtg
     4561 agcgggacgg ctcttttcgg acagagtaac ctcaaagcag cgattcggaa gatgcgcgaa
     4621 gcggcgatgc ggcacgcgcc agcgccgcgg ggcggcgcca gaacttgagt catttgccac
     4681 agatttcgtc tggggagcaa ctctgacccg gaagcctcat gttggtgacg agctgcccga
     4741 aggcggcaca ccgaacagcg cgccgaagcc ccagacgcag ttgggtgtcc cggcttcagc
     4801 cggttctcgc cggggaccca ggccaatacg agaactgcgg aactgcggca tggccatttg
     4861 cagcgtttga cagccggtcg gacgctggta tagtcctcga ctctttatgc acgctcaacc
     4921 gcatgttgcc gttgtcggcg cgacgggcgc cgtcggtgtc gagatgatga aatcgctcga
     4981 acgtcggcgt ttcccgctgc gcgaactcac cttgctggcc tccgcccggt cggtcggaaa
     5041 gaccttcgag ttccaaggca agacgatcgc ggtgaaggag ctgaaacccg actcgtttgc
     5101 gggggtcgac atcgcgctgt tcagcgccgg agggagcgtt tcgagggagt tcgctcctgt
     5161 tgccgcccgg gcgggatgcg tggtgatcga caattcgagc gcttttcgga tggacgactc
     5221 ggttccgctg gtggtccccg agatcaacgc atcggatgtg cgaagtcaca aaggcatcat
     5281 tgccaatccg aactgcacga cggcgattgc gctgatggcc atttacccgc tgcacaaggc
     5341 gttcttctgc aagcgcattt tcgcgtcgag ttatcaggcg gtgtcaggga ccggggcgaa
     5401 ggcgatcgag gaactgcggc ggcaggtgga tcagatcgtt cacggccagc cggtgacgaa
     5461 ggaggtctat ccgcatcaga tcgcattcaa tgtcctgccg cacatcgact cttttctgcc
     5521 ttccggctac acgaaagagg aaatgaagat ggagaacgag ggacgcaaga tcatgcatca
     5581 ccccaccttc cgggccagcg tgacgtgcgt ccgtgtgccg gtctaccggg cccactcgat
     5641 cgccgtcagc gccgaattcg agaagccggt gacactcgat gccgcacggg ccgcgctggc
     5701 ccaagcgcct ggactcgatg tggttgacga tccggcgaac aaggtctacc cgatgccgct
     5761 gttcacggcg gagaaggaca actgcgaggt ggggcgcctt cgactggatt gcgccctcga
     5821 caacggcctt tgcttctggg tctcgggcga tcagttggtc aagggggccg cgctcaacgc
     5881 agtgcagatt gccgaggagt tgctcaagcc ttagggtctg tgcatagatt acttccgatt
     5941 agtttttgca cagaccctta gcccgtttca tagcggtgcg cgtgacctga tgtccgggct
     6001 gtggcgctaa cgcaggcggc acagaggact gcggcctcga ggccgtccga gcgcgatcgc
     6061 ctttcgcctc ccccgttttc gtggtacggt gggctgcgga ttgagttggc catgttcgac
     6121 ctgcgtgatc tcgagcgatg cgacccgact gcacaaacgg tgcggtcgag ggcttggggg
     6181 tccggcttta cgcttgttga actgctggtg gtgatcgcga tcattgcgat tctggcggcg
     6241 ctgctcctgc cggctctcgg gcgcgcgaag ggcaaggccc ggcagatcgc ctgcatcaat
     6301 aacctgaagc aactggccac tgccgggacg ctctacgccg ggacgccaac gaccatgtcc
     6361 ctcccaacgg ttttgggtcc tcggacatcc tgaatgggca gagactgtgg gtgttgggcg
     6421 aggagcacta caaattcacg tcgccgcctt cacccaatcc cgccttcacg aatctgaatt
     6481 atctgaacga tccccagcac gccgcattcg cacagtatat cccgtcggtc gcaacctacc
     6541 ggtgtcccga ggaccgcacg acggtcgagt tgaatggccg cgcccatccc aaggtgcgca
     6601 cctatgccct caacagctat ctgggctggg ccgagtgggt ccctccgggc tacaacagca
     6661 cgcgtcactg gacgttcgag aagtggtcgg ctctggcaga agcttccccg accaccatct
     6721 tctccttcat tgacgtcgct ccgggcaatg tctgcctgcc ggcctttgtg gtgcgcatgg
     6781 gcgcgggtgg gcagttctac cacctcccct cgatccagca cggtggccgg ggtgcggtgg
     6841 ccttcggcga cggccacgtc gagacccgcc gttggatcga gagccgcacc ctcaccgaag
     6901 cggccctgcc atggaatccg aaccactgga ccctctggga cccgggcaac cgcgatctcg
     6961 agtggctcca ggaccacgcc accgttccgc gtccaagcgc ggaataatcg tcccgatcga
     7021 ggcgccctgg accagaacga acgggcgcgc ggtgggcgcg ggcgttacca ggcccctgtg
     7081 gcgggcgttc accttgtcca ggccctccga catttggttg cggctctgcg ccgattcagg
     7141 ctctgcatgt cctctgtggt gaatccctgc cgcgtaaatg ccttatctga tgatcaagga
     7201 cgcggcttcg ccctggacac cacgggattc ctggagatgc aagatacttc tttacttgac
     7261 gccgagcctc gaggattgtg acgcatcgtc gtgagcaaaa ctcggccccg tgccgtgaga
     7321 accttcgggg ttcctatact cacgactttt tctttttccc cttcggcggc gggaaacgtc
     7381 tgtccagcgt cagcggctcg cgcgctgtga agaacgttcc gtgtgcgcgc gcgaaatgga
     7441 ggtcatgcac ctcgcccacc tgcggccaca tcgccctcag gaagcggtct tcgtggatga
     7501 caaggcccgt ggtcggatcg agggccttct ttcgcgtctt gacgtcccac ttcacttcgt
     7561 agaaccgctg cagtgcccag aggaagtaat ccacagcctg aaggcatggc gcttccttgg
     7621 agcggaagac tgtgatcttc catgcgtcag ggctgctccg ccggaatccg aacttgcgct
     7681 cgaaatcctg ttctgcgtgg gcgagggcga ctctgatggc ttcatttcgt gtgctcgtgc
     7741 cacgcctggc aacgttgact tcgtagcggt cggccaggcg atggaacttg ccgaacaact
     7801 cccgcatgag cgcgtcatac acggagttct cgttgaagcg ataccccggt tccttcgccc
     7861 gtttggcgct ttcggtttcc gtgagtttca acttgtcgca gacgaccgcg tagaagcgca
     7921 attggctccc gaagctgcga agcagttcga aaacggggac gcggacttcg ggcaggtcgt
     7981 ccttggcgtg gaacgcgatc gccgtcttct tgcgctccgg cttgaaagat tcagttccgg
     8041 cgaagatggg gtccgccaac atcctctggc ggagctccgt cagcgcggtg gcgacgacgg
     8101 ggggattctc gacttcgagt ttgccgagaa tgaagaaccg cgagcagcca aaggtatcgg
     8161 cgatgggttt gccgctctcg tgaaacagtg tgggcgtgcc ggcctcgtcc acgaagagat
     8221 gctggacttc cgatgaactc ggctcagggt cggccgggat gtcgagaagg tcgttcacta
     8281 tgtcgtcact tcagcggcca gccgccgtgc tgggcgatga tcgcgtcaac ctcgcccatg
     8341 aggcggatgg tttccgagag agcgacgacg attctgtggt agtgggtgat gtcatctgcg
     8401 gacagcacgt ggcccttccg gtctttgagc cacttctcgc agacctggta gccgccgatg
     8461 tggaagttcc agaccggctc cggcacacca cgaaaaccgc aggtcttggc cttgccaaac
     8521 cagaccgtgc ccgcttcgta ggtgactttg tccacgacgg gcgaggccga gccgatgaac
     8581 ttggtgatgg gcttctccag cgttggcgac tccagcagat gcagggcgac gagttcgccg
     8641 ccgaggtggg ccagtgcgcg gaacagttcc aagctggaag tcagcggcag acgcgggaaa
     8701 tctatcttca gaaactcagc gtagcgactc cggtagccag gactgtgaaa caccgcgtag
     8761 gcgtagtgga agatgtcctc ggcgctaatg ccggagggca aaccatccga agcccgctgc
     8821 acaagattca gtgcgtcggc aaggcttctt acgaagcgcg aactgaggtt gacttggggc
     8881 gcgtgatgtt ctcgaaggag gctccctgct tgagaatcat cggggggcag cataagagga
     8941 aagagataat ttacctcctt caccgacacg gagtggtgct ggatgaatcc ccgagtgcaa
     9001 aacacgtgtt caaagccacg accaatttct actgtcctcg tcgtgaccat ggcgacattc
     9061 tttccgccta ccatgcaacg catcacgtcc catacgggtc ggcacacgaa gcccgatgat
     9121 accccagtgt agtaaacgtg ccgcacatca aacggtcgat acaagacttc gcgcacgaga
     9181 cttcggtcag gcccgctctt tcggacgtcc tcctgcgccc agcacactct ccaactttgg
     9241 acatctgatc ccaattcata ttcggcacgc gcatcctccg gagcgagcct gcagaacctc
     9301 actacgcgct gccaaagccg accagcgtcc acgtcgattg tgagggcgtc tcgcgcggtg
     9361 actatcccga ccccgttttt cggaaacact tccgcaaggc tatatccacg ttcatattca
     9421 gctcggcaat ccgagttttg agggatgaac agatagaacg gtgaattggg ggataacctc
     9481 tggaaagcgg tattgtcaac atcgtgatta gtcaaccatg cgtatttggc ctcccgcagc
     9541 ccccaaaggg ccgcgtgttg aacagaggat tctgcaagac tgcgagttgc taggcacacg
     9601 gcaacgccct gtcggatgtc gaagacgttc ttgtccagac catcgggggc gagctccttc
     9661 ttgttggcgt tcccgtggag gtcgagtagg cgaaggcgtg caaatgtgct tactaagctc
     9721 tggcgcatcc cccggaaggt tgggttgtct aagtagccgt ggttcgtgac atagccgagg
     9781 acacctaccc cagccgcatc gattattgtc tgagctaggc ggatgaactt cacatagtcg
     9841 tcctggagcc atagtttccg ctcagataat gctactccat cgacgacttt gtaggcatca
     9901 atgagccgtt gagcgtgatc ggtcatgttc gacgagattc ctgcatacgg cggattgcca
     9961 acgacaaccg tgaatcgctt ataccacttg atttcgttga ccgcctgggc ctcatgagcg
    10021 agcgcgtcga aaccaatctg cgggagttgt ttaactttcg gttcgagcga gttggtgagg
    10081 tagatgcgga cgcgctcggc gctgccgaac cggtagccgg tctcgtagag cttgagaccg
    10141 accttcatgt gggcgatggc gtagggcgcc atcatgagtt cgaatccgta gaggcgtggg
    10201 agcaggcagg cggggacgta gacgttccaa tactggttga aggttgaggg ctgggcccca
    10261 ggaccctcac ccggcgctgg cgcgccagcc tctcccgccc ccccgggcga gggaatacgc
    10321 ggcatggcgg cgaggccgcc cttgttccac tgcgtcttca ggtgccggtg gaccacatca
    10381 atcacctcga cgatgaaggt ggctgtgccg gtggcgatgt cgaggatctg aacgaagaac
    10441 tcgccggggt caatggtgcg catctcgccc ggctcgtccg tccgcggcgg aagtttgagt
    10501 tcgggattcc gctgcaccat ctctccccac gtcaccgtgg aagcgaggcc agcggcgagg
    10561 ccgaactcgg tttgcagcag ttcatgaacg ctgcggacga tgtaggagac gaccggcggc
    10621 ggcgtgtaga agacaccgcg ctgcaccttg agcttcttgt tgtaggcgga caggaagtgt
    10681 tcgtaaaagt ggatgaccgg gtcttcgtcg gggttcttgt tgccgaagtc gcgcagaatg
    10741 gcaggcaggt ccgtctcgtc gcctcggagg agttccacca cgtcttgaat gccgagttcg
    10801 tcgaaatcaa tgccgccctt gcgcccgccc gccttcagga aagtctggag catttccttc
    10861 aggaacggat tcgtgacggg cacgatgtcg gaaacgtcct ccgcccggac aaatgtgcct
    10921 gcggtacccg cgctcatctc ggtgcgtgag atggcggcgg tgagcaggcc gtaggtgatg
    10981 gtctgcgcgt aggtgtcggc gaaactctcg ggggtgaggt cgtgaatgag ggcagtctgg
    11041 aaggctttgt agagtttggt gagggcgccg cgctcggtct cgtgcgccat catggtctgc
    11101 gcggcggcgc agatgcctcg tgccagggcc gcgaggcgtt cggccaggcc gtcggcggtc
    11161 cgaatgacat ggcccacccg gtggcggaag gcgcgggtcc attgagagcg ccacgaggac
    11221 aggtcgcgcg ggtccgccgg ccagcggagt ttgtcatgca gagtggcgtc cacgtgctcg
    11281 agcttgagcg gggtgtcgcg gccgtcccag cccagcacgc ggagcgtggg gaggtcgccc
    11341 gattcctggt ggaaatgggc gaaggcgatt tcgcgctggt ccgtggcctc gtcgccgaaa
    11401 gcggacacga acagcaggtc ctccggctgc caggcggcac gctcggattt gttcgccgag
    11461 ccgcgcttct tgatgacgag caggctgagg atgcggcgaa ggaccacgac gggcaggcgt
    11521 ttcttctcga actcgatgaa gaagatgccc cacggctggc ccgaggcgag cgggcggagt
    11581 tggtgaattc tcttcacctt ggccgcatcc tcttcgcgca agcccaattc ctcggcctcg
    11641 taggcgaacg tgagttcctc gaagccgtat tgctgaagcg gccaggcgag ttcctcctca
    11701 aggaagcgga tgaggtcggc gaagttgcgg agagaacgga gacggtcgcg gggagccata
    11761 ggtcatgaag cttctggtcc gattgccggc cattggtcag gcgcgaagcc gagcgccaag
    11821 agcaggagac gatactcctt caccagcttg cgaaaagacc gggaccgctc gtggacgcgc
    11881 cgaaggttga tcctggcggc gaacttcggt tgatcagcgg gtgcgtggta gtacatcgcc
    11941 agccgccgtt ccagttcttc cttggctccc cgtttctggt cggggtcgcc gggatatgac
    12001 gcccttcccg cagggcggtc ggacaggtct tccagcgagg ccaggaacca agcctcgaac
    12061 tccttgttgg cgagcaccac cggacaggga atcgcggcgg caatgcgtgc ggcttcctgc
    12121 tgcagaggta tggcgcccgt cttcggacaa aggtcgtcgg catcgaacag gacaaggatg
    12181 ccgcggcaac cggtttggcg gcgcgcgacg tcaatggcct tcgccaatcc ttcgcgttgg
    12241 atcagatgcc cccggccgcg gcggatcggg cggcccaacg ggacgtgggc gacatccatc
    12301 tgcgcggcca acctccggag cagcacgggc acggcttcga tttcgccgac accttcgacg
    12361 atcggctgaa tcatggcgtt gcgtcggcga acagctccat ctggttcgga tcctgtgcgt
    12421 cggcaaagaa gtccaccgga tgcagcgcgt tggatcggag cagttctccg gcgcccatca
    12481 gatggtcccg caaggcggcc acggaggcct cgcccaaggg cagaacctgc gtcatgccct
    12541 tgtcccagga aacgacacga aggttctccg gttcaatcca cttggcgtca agcaactcag
    12601 ggctgtgggt cgtgacgagc acttgggtgt cgcgcgagaa ggaccggatc atttccagaa
    12661 tggacgagag cgcctccggg tggatggtgg actccggttc ctccaggccg acgagggtcg
    12721 agcggttggg ctggaggaag gccgtgagga tgcccagggt gcgcaaggtg ccgtcggaca
    12781 tcgagaaggc cccatgggcg acggtgcgtt tgctggcggc tctgcgcgat ttgcctcgcg
    12841 gcccgccgtt ctcgctccac tcctgacgga actccaaaga gagcttcttg ccgtgcttga
    12901 tggggcgaac agtcgctgta cctggcacca cggtgcacaa caactcacac aggcgctcca
    12961 tcttctcctc gtccttcgac agcgacttca agacactggc cagattgctg ccgtcggctt
    13021 tgagaatgga accggaatcc gggtcttgca gctccagaat cgccttgggc tggatggaaa
    13081 acacgcgaat gccgctcaag cccctggtca cgggcgcgaa ttgcgccgtg cctcccatca
    13141 ggggcaggag cagcgcctgc ggattcacgg ccggctcgat gctgggcaca ctggttgtga
    13201 ccgaaccgtg aaaacgatcg aaccaactca attggccgcc cgtctgcacc acgcatttct
    13261 cgcgcaccac gtcgaactcg tagccttcct tggcgcgaat ctcgaaggca taccatcccg
    13321 cagatcgtcc gccgttgcgg aaatcgaaat ccacacgcac ggccatgttc ggcgggcggc
    13381 tcttcgtgcc ggaacggtgg cggacggctt caatgccgcc ctggttgttg aagaccgcac
    13441 cgagcggcgt ggtcatgcat tcactgagga actcgagcgc cttgagaaca ttgctcttgc
    13501 cggcgccgtt cttgccgacc aggaacaggg ggttctcgaa cccgaggcgt gccgcgagga
    13561 tgctgcgaaa ccgcttgagc cagagctttc tgatgggcac gaattcagag cgcatgggca
    13621 ccttatatcg tcgcgaaggc gggcagtcag ttcaattcca tccagcattg caggacggga
    13681 ttcaccccga cgggcgcctg aacctgcttc agataggtgt tcttgatgtg cttctcgacc
    13741 ttcagccgca cctcatggag gtcggcctgc ggcatggcca cgcgccgggg cgtttcgggc
    13801 gtgctgcgga tgaacgcgac gatttcctcc ggctgctcca ggatcttgcc ggtggcaagg
    13861 tcatacaggt accaaccggc cttgcctgtt ccggtggtcc aagcggctgc tccctcagcc
    13921 tggcctgctc ccccgccggt ggcggcggct gccgtgtcgc tgcgtagggc tttgtcctca
    13981 gcgggcaggg cgtagcacag gaagagggcc ttcgcgcccg gcttcgtgtg ttccttgccg
    14041 ctgaaaaccc ggccgggcaa ctgatcgagc ctgtgttgca gtccggggtt gtcggcgagc
    14101 agtttctgga actcgaggtg catctgctcc aacggtgtga gcgcgccctg ataggcgtgg
    14161 gtgaaatcgc gcagggcctc gtaatcatcc tccggtttga gcagtttctt gccttcaatg
    14221 ccgaagacct tggaaatgcg cagcgtcttg ttcgacaccc ggtggtaaag ccggagaagg
    14281 tcgtcgagtt cgtcgggggg gagaaaattc cagtaaacaa ccttgccgcg cagcggcgca
    14341 tggtctgggt ggtccgcaac gattctggct tcaatctccg ggttcagacg gcggtccacg
    14401 cggccaatgc gctgcatcag acggacggga ttccaatgga ggtcgtagtt gatgaggcgc
    14461 gtggcatcct ggaggttcaa gccctcggaa agcacgtccg tcgaaatgag gatgcgggtt
    14521 tctttctggc ccgcggcggc cagttcggcg ctcgtcgtgc cgttgtaata gggcgcaaac
    14581 cgctggatgg catccccacg gtcggtcttg ctgccgctgt cgatttcgtc cacgccctcg
    14641 atgcccgcgc cctgcaattc gcgccgcacg tagcgggcgg tggtcatgaa ctcggagaaa
    14701 atgaggacct tgtgtttctt cagcaccgcg tcggacttga ggagtttgat gagggactgc
    14761 agcttgttgt cgtggctggc gtcgaacgtc ttgagttctt cgagaaagtc caccaactgt
    14821 tccaggtccg cataggtttc gtcgaagatt tccggaaggt cgtagttgtt gcggtcgaga
    14881 acctcgaagt catcgaggaa ttcgtcgccg agttctgacg caccaacttc ctcgctctcg
    14941 tcttcttcct ggaactcggc gcggcgctgt ttgacatgat cgagcagttc gccgttctgg
    15001 gcctcccaac gctggaggcg tttcgtttca gactgcgcca tcgagtttct tcgcaagaac
    15061 gccagcaact tcaggagcag attctggcag gaggtctcga acgcgtaaat ggaactctcg
    15121 aagcgtttca ggaactggat gcggatgagg cgaacaacct gcttctggcg attctcatcg
    15181 aagacattga gggccgccgg gtcgcctctc cattttgcca acgggtagta caccgccagg
    15241 ctgaaaagct gctcgggttt ggaaaaggcg gcttcgacct tgcccagcag gtggccgtag
    15301 gtcttcttga ctgagtacgc cgcgactctt ggcggttcct tggccggaaa gacagcctcg
    15361 gtgccgccgt gctgaatctg gctctgctgg gcgtaggccc gactgcgttg cacgaccagt
    15421 gcacggaaga gctggtcgtc gaacaggact ttctcggcct ccgcctgatt aacctggaac
    15481 aactcgccat gctcctgccc tgccacaagg cgcttcatcg ctttttcgag ttgctggaaa
    15541 tggctggcaa ggttgtggat gccaagcgca ccggcaaacg ccgccggatt gtccacccgg
    15601 ctgaacagtt caatctggtg ctggaaatct gtcagcgcat tgttgacagg tgtggcggtg
    15661 agcaaaaaga gggtcttctt ccccacgatg tcgtaaagct gccagtagcg ggaacggatg
    15721 gtgccgtttt ccgcgttggc caggccgcga ttgcgaaagt ggtgcgcttc gtcaatcacc
    15781 accacgtccg cctgttcctt gatctgctgg aactcggagg ggaaatcgcg gccgcccgcg
    15841 ctggggccgc gcatgaggtc ggtgtggttg tagattttga agggcaggaa gccgttcaga
    15901 agatggggca ggtaacgtcg gatattgcgc tcccacacag ccacgcggcc agacttggga
    15961 accaacaaca tgacgcgctt cttgtcgcac atgaccaacc gctcgatcag catcaagccg
    16021 acgaatgtct tgcccagacc gacgccatcg cacaggaaag cgccgttgtg ctggcgggcg
    16081 atgttgacca gcgagtggta gccctccttc tggtagtggt cgagaacggg atagatcttg
    16141 gattcgccgt tctcccactc ggcttcgctc ggtgtgtgat gctcgaacag cccctggaga
    16201 gcccgagcgt aaacctcgaa cggcgcgtat tcggcaatgt gacgtgcaat taccttgatg
    16261 acgtcgggcg tgatttcctc cgcttcctgc cagtgctgct cgtaccaggt ctgaagttgc
    16321 gaaacatcgc cgggcgcacg gatttggatg ttgagttcga cgttctgcgt caggcccggc
    16381 acggtgaagt tgctggagcc aacaagcgcc actgacccga tgaccgccac gcgtgggcgg
    16441 gtgatatagg ctttggcgtg gaacttacgc ctcgcgtaaa cccgacactg gatcctccct
    16501 tcccgcatcg cctccacgat cgccgccgcg ccggtgagca agtcgttcgc ttccttctcg
    16561 ccctcaagac tggcgtcgag tttctggcag acctgctgcc gtagcccttc gagaatggcc
    16621 tgccgcgtcc gcgccgtggc ctgatcgccc atcaagatgc ggatcttatc gagcttttgc
    16681 catctgccgt cgagggcgag aagcgacccg atttcaaaga agccggtggc gatgtcgaag
    16741 gcgctggcaa tctccgtcca gtcgtggagg tagcgcagtc ctttccagga ctcgtcgctg
    16801 ttgtccacga tgaacagttc accaccgctg ggacgaggga aaatcactgt gtgggtacga
    16861 ccaagttgct gaggcccacc agggccgccc agaaccgaca ggcatttgct tcttcgactg
    16921 gctgaagtgt gccagtccaa gagatgccat tcaaggagaa tcccggcaga gttgccttgc
    16981 tcacacggtt atttcattta aattcctgaa gggttgctgt tgttgaggtt gtaaataacg
    17041 cttgtcaatt ggcgtggttt ggtgcgtagt aggcgtgcga tgatctcgac aaaccacca
//
LOCUS       DAOQRO010000020        16132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2694_length_16132_cov_5.270759, whole genome shotgun sequence.
ACCESSION   DAOQRO010000020 DAOQRO010000000
VERSION     DAOQRO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_16132_cov_5.270759"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKK20_01555"
     CDS             complement(<1..397)
                     /locus_tag="PKK20_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNU98607.1"
                     /translation="MTCQGCARNATQAIQGVAGVANAVVDLTAARATVWWEPGRTADD
                     KAVVAAVQRAGFGATALDAENVDVPGTSGDRASHGIEVWSPLAGWRFNVVVGGAVTLV
                     LMVGEWVLGLGMTRWFQWLAFGLALPVQIA"
     gene            608..1426
                     /locus_tag="PKK20_01560"
     CDS             608..1426
                     /locus_tag="PKK20_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU98608.1"
                     /translation="MNLEDHLGDILRKGRLHAGISLEPAAAAASLTIPELKTLEESGA
                     ASRPYNLEALAALVGLDAPKLRAIAEGWNPRPVNLDTWRQFRVITTAAEMSVNAYLAW
                     DEVTRDAALFDTGFDPAPVFALIQEHGLEMRHLFITHGHVDHVAALPAIRARFPKMRL
                     HSSSRTASVDQRNRPDECLQLGSLRITHRNTPGHAEDGVTYVIGTWPDDAPHVAIVGD
                     ALFAGSMGRATGQALLAKQRIRECILTLPPATLLCPGHGPLTTVAEERAHNPFF"
     gene            complement(1492..1923)
                     /locus_tag="PKK20_01565"
     CDS             complement(1492..1923)
                     /locus_tag="PKK20_01565"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNU98609.1"
                     /translation="MTPPILQSRPDSEPEGWTGPWPAWLFLGLLGLLVGARFAELWRL
                     PLPHCWFKTITGLPCAFCGATRAASAAAGLDLATAIRLNPLACALGAAVLALFVAWGA
                     DRWLGTRWGLWLRGRASWPWYWIGLAALVLNWIYLLAARDG"
     gene            complement(1886..2278)
                     /locus_tag="PKK20_01570"
     CDS             complement(1886..2278)
                     /locus_tag="PKK20_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98610.1"
                     /translation="MKTSSDRAGQEPIDKPTAWACTITNAAALPGLGTIAGGCKVGYV
                     QAACALSGFALSLGGLVAHLRVWFDTGEMPEGFTRGLMVAVLGLGLFASAWLWALASS
                     IRMHRRAARPSPAPKSNDAPHSPIPPRL"
     gene            complement(2235..3224)
                     /locus_tag="PKK20_01575"
     CDS             complement(2235..3224)
                     /locus_tag="PKK20_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HNU98611.1"
                     /translation="MFKMIGGDGREYGPATVEELSVWILDHRANGQTMVQVEGSSEWQ
                     PLSTVPEFAEVLRRAYPDAAAGPTRSESSTHGTIGTIEAWNAVPAATLSAGDCLGRGW
                     VFLRRHFLLVSAGCALVWAILTLSAMATCIGGVLGLILSGALYGGLMLLYLRLMRGQP
                     ASLGTLFTCFGPRFVPLMLIAIVTQVFSQIGLLFCLVPGLILKVIWAFALPLAADRGL
                     VFWPALELSRRTAQRHFFNLALLMCVAFLPLLVFEVYGGLRMMTFLTDVLGPPGTWTL
                     DAIQDKKSQLGEFMMTLGLQEQLVVLLSMPFGYAALLQAYEDLFGPRRAGADR"
     gene            complement(3330..4844)
                     /locus_tag="PKK20_01580"
     CDS             complement(3330..4844)
                     /locus_tag="PKK20_01580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNU98612.1"
                     /translation="MTGVGYSDLLAALAPETALVLAAVAVLFVDMGLMRSAPLRQRFL
                     TACAVAGLGCLVTLGILASSGAHLRFLDGVLVVDSLTQWVKVFLVVLTLFTAGISAQA
                     EFTEHVGEYFALVLLATVGMMFLVSTEEILMIFVSLELTSLSLYVLAALDKRTRHASE
                     AALKYFLFGSVAAAFTLFGLSLVYGLSGATNLTGIAAGLRAHRSDPLLAVAVVMVIMG
                     FGFKVAVVPFHLWAPDAYEGAPVPAAALIASGSKVASFFVLAKIAVLGFAGVEGSGAW
                     GQLRAGWLPVLAVLAVGSMVLGNLTAIAQGHLRRLLAYSAIAHAGYVLLGLMAMGYPS
                     QRSAALAAVLYYVATYGLTTVGAFGVASVIERVLGNGRIDALAGLSRRSPLLALCLMV
                     FLLSLAGIPPLAGFFGKFGLFTAAMGADPAGLGLLWLVGIAIAMSTVSLYYYLRVLKQ
                     AYVVAGDEEASRIVVPWTDRLVLIAAAVGVFLLGALPELLLGPLARAIAGGNGF"
     gene            complement(4841..6340)
                     /locus_tag="PKK20_01585"
     CDS             complement(4841..6340)
                     /locus_tag="PKK20_01585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNU98613.1"
                     /translation="MFAWTIYISFAGVAVLMLLPRDQGGRARWVALGTALAGAIAAFG
                     GAFQYQAGSGLQTIARTSWIPSLGIDYHLAVDGISVTLILLTAVAAVAGILFSWNITG
                     RAKEFFAFYMALIGGVYGVFLSFDAFVLFVFYEIAIIPKYFLIAIWGSTRKEYGAMKL
                     ALYSFAGSALVLIGLIGTFVVARSQTGVPTFDLTALAQVDYPESFQRWALPLVFVGFG
                     ILAGLWPFHTWAPTGHVAAPTGASMLLAGVVMKLGAYGCLRVAMTLFPAGLRHWQTEI
                     AVLAVVGIVAGAMTALVQKDFKFVIGYSSVSHMGFVLLGLATLNEVGLSGAVLQMFSH
                     GVIAGLLFAVVGRMVYDRTHTRQLDELEGMRLARAMPFAAVTFVLAGMASMGLPGFSG
                     FVAELQVLIGSWRAFPTAGLAAGVGIVLGVAYLLRALQRAFFADSAGDARGEPAGEAI
                     EPISLPERAGAILLLGATLAIGLYPRLLLDFIAPSVTSLIQGLERGGGS"
     gene            complement(6343..7902)
                     /locus_tag="PKK20_01590"
     CDS             complement(6343..7902)
                     /locus_tag="PKK20_01590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNU98614.1"
                     /translation="MNPFGSITLLTLIPLVGGLVVAALPAGQKTLARRLGLGVSLLAL
                     AVAGALWSGFDRDSTALQYVERRAWVPTLAVEYFVGVDGMGLLMVMLAALLVPLALLV
                     SWRIEERVPLYFALVLWLEAGLFGAFTALNFFHWFLFWELSLIPAFFLVRLWGGPDRG
                     SAATQFFIYTMAGSVGLLLAFLALFLATGKFDFLELADLGRTHQTAALLHAHLGWSGM
                     SADALAQAVFFGAFLGFAVKVPVLPFHTWLPSTYAAAPTGTTMLLTGALSKMGVYGFL
                     RILLPIFPDQMRAWETPLLGLAVLTIVCSAAAAFAQRDVKRMLAYSSVNHLGYCLLGM
                     FAALRVTGEEARWAVEKAAALNGVLLQMFNHGLTAAMLFGFVALIERRAGGLRGLDDF
                     GGLRKPAPVLAGLMGVAAFGSLGLPGLNGFVGEFLIFKGTFALAPWVAALSTAGLLIT
                     AVFLLTFLERVFHGPLPARWAAFPDLSRAEIWMVAPATALMFVLGIYPQLVIGVIHRT
                     VLLWVERLGPC"
     gene            complement(7902..9983)
                     /gene="nuoL"
                     /locus_tag="PKK20_01595"
     CDS             complement(7902..9983)
                     /gene="nuoL"
                     /locus_tag="PKK20_01595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNU98615.1"
                     /translation="MNAITPYLWLIPALPLAAAGVIALLRQRRKTLAAGLAVGSIGLS
                     LILSFIAFWTTLGGASQGHGGPAHDTSRFVWFALGPTNLELGWVLDPLAASMLVMVTL
                     VGLLIFVYSVGYMAHDENFTRFFSFLALFAAAMLGLVIANNLLLLFICWELVGLTSYL
                     LIGFWFHKPSAAAAAKKAFLTTRVGDIGFLLAIVWLYAQTGTLQFYDDGAGCLEHSAL
                     TRLVTQTTTGGLGVATAISLLLFCGAIGKSGQLPLHVWLPDAMEGPTPVSALIHAATM
                     VAAGVFLVARVYPLMAVHPDGAAEATTALQAVTWVGALTAVFAASIAVAQTDIKRILA
                     YSTVSQLGFMMMGLGVGGVAVGMFHLITHAFFKALLFLGAGSVIHGVHEEQDIRRMGG
                     LRRWMPVTFATYGVGMMALAGVPLFFSGFWSKDAILHAAHGWTVSAAPFYLGLCGALL
                     TAFYMTRQVCYVFGGAYRGGAGRAATAHASRRDTPHESPAVMTAPLIVLAVFAVVLGF
                     VGTPVWPWFQGYLTGESVEFHASALLSREHWPMIALSALIVLTGFGLGWYWYGRQPIT
                     SPEAPDAIERYQPTFFAVLRGKYYVDEFYARTVVRWHAAWAWTCDTFDRWVWGGVVYA
                     ISAVVVGCAWLTRALDDYGINLGFDRACRQIARGGQAGSRFQSGQVHDYLRALGMALV
                     VLLLLFIWGDS"
     gene            complement(9980..10291)
                     /gene="nuoK"
                     /locus_tag="PKK20_01600"
     CDS             complement(9980..10291)
                     /gene="nuoK"
                     /locus_tag="PKK20_01600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNU98616.1"
                     /translation="MGELARYLVLAALLFGIGLAGVLTRRNAIVVLIGIELMLNAANL
                     NFIAFWRHGPHPEALTGVMFVLFAIAIAAAEAAVGLALVIAVYRHYRTTNLDQIEEMK
                     G"
     gene            complement(10295..10843)
                     /locus_tag="PKK20_01605"
     CDS             complement(10295..10843)
                     /locus_tag="PKK20_01605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNU98617.1"
                     /translation="MDIAFLITAAAILAGAAGAMAARSVVHCALCGVVAFASLAVAFL
                     QLRAEFVGFAQLLVYVGAVAILIVVALLLTRNFEATHEALSFRPWALGLAIALLVSGA
                     VVSAILGSGLGVGAPPSAPPPTVKAIGDLLMTRYVVALEAMGVLLTVALIGAALVALR
                     ENGPGRPAKPGEQGSVAGRLEE"
     gene            complement(10857..12053)
                     /gene="nuoH"
                     /locus_tag="PKK20_01610"
     CDS             complement(10857..12053)
                     /gene="nuoH"
                     /locus_tag="PKK20_01610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNU98618.1"
                     /translation="MFEALDQLFVSLKHWLVGQLPPAWQSWVSLAISAAAILGVFMTL
                     FALTVLIERKGLGRIQNRYGPNRVGPWGLLQPIADLIKMLTKEDLVPSGADRVVHFVA
                     PVVMVVPVLMAYAVIPIGRNMAAVDFNAGLLFFFAVGACIELAVFMAGWSSRNKYSLL
                     GGMRAIAQMISYELPLILSTLPVVMLAGSLSLVDITDAQAGHSGGWLARWHVFTPWGL
                     AGLLLFTVAATAESNRAPFDLPEGESEIIAGHLVEYSGIKYALFFIGEYLGMFAVSAL
                     GITLFLGGWRAPVIWLEWVPSYMWFFLKLGGFVALFIWLRGTLPRLRADHLMNLAWKF
                     LLPLVLLNAVSAALWHYTAPWTFVGAGLARWLLCGSLVGVPYVLLGRRLFAGQHIGPR
                     TYRMAA"
     gene            complement(12134..12736)
                     /locus_tag="PKK20_01615"
     CDS             complement(12134..12736)
                     /locus_tag="PKK20_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNU98619.1"
                     /translation="MNLGKGILKGLVETARNFVGSYYDPARLVTIQYPDERRALPEKA
                     RSFPMLVYDGEDPEKGLRCVACKICEKECPPQCIYIVLDRDEKGRPLRRPKIFDLDIS
                     VCMSCQICVEVCPFDAIKMNTEFELSTADRFGSLLLHKMDLARPASYYLGLHPVEAGE
                     VNARLAAGPGHAETKAKPAAGPAPAAGTTSGAKPGDSDRP"
     gene            complement(12733..13992)
                     /locus_tag="PKK20_01620"
     CDS             complement(12733..13992)
                     /locus_tag="PKK20_01620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNU98620.1"
                     /translation="MNRRIPEDVLIEALSTGDLVPESPSHAAVLPRPEGAPRTASEAK
                     PHPIALGDSEGTLLEVAVGPHHPSTHGVFRMDVVLDGERVVRLKPVFGYLHRNHEKLG
                     EGGAYLAAMPYTDRLDYFCSLTNNWAYALAVERLAGIEVPERAEHIRVITAELTRVLN
                     HACLIGFLLQELGSMGTPIMYAFREREKILELIEALTGSRMMCNYMRFGGCRCDLPSG
                     WIEACRDVVEALPRFLDEFENLLSTNEILMARTQGIGVLSAEQAVQAGMTGPMLRASG
                     VNYDLRKVDRYGVYDRFEFGVPLGAHGDVYDRYMIRMLEMRESVRILQQALRAVPAGP
                     IINPKARLRGFKPPAGEVYGRIEAPKGELGFYLISDGSANPYRYRIRPPSFINLTILE
                     DMCRGATLQDTVAILGAVDIVMGEVDR"
     gene            complement(13989..14624)
                     /locus_tag="PKK20_01625"
     CDS             complement(13989..14624)
                     /locus_tag="PKK20_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNU98621.1"
                     /translation="METLEQIKSRLEAAIPGLGLEIQCNGAVPGQASLVVPPARAIEA
                     ARFLRDDPRLALDYCSNVSGVDWLDRVVEEHARVTRVIDGRERVFDECRERRVPGYLE
                     AVYHLYSMRLKHGPVILRQRTVNRVDQARVTSLTPVWRSAEFQEREAYDLFGIVFEGH
                     PDLRRILMWDEFRDYPMRKDYVEPDDYEYEPTPHDDLKEKARRHYPIEADA"
     gene            complement(14667..15290)
                     /locus_tag="PKK20_01630"
     CDS             complement(14667..15290)
                     /locus_tag="PKK20_01630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNU98622.1"
                     /translation="MSTMDEGLRSELSRQGIFATTIQDLYNWGCRNSIWPLNFGLACC
                     AIEMIAASMARFDIARFGAEVFRPSPRQADLMIVAGTVTKKMAPQVVRLYNQMPEPKY
                     VIAMGACAISGGPFKRGYNVLKGIDRYVPVDVHIPGCPPCPEALLHGLIALQKEIASQ
                     KLTGPSRARAFQEEAPGEFPVPEYGPYDLVPAKNTDLWRPKPIIRDE"
     gene            complement(15340..15717)
                     /locus_tag="PKK20_01635"
     CDS             complement(15340..15717)
                     /locus_tag="PKK20_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNU98623.1"
                     /translation="MPSAPHPYLFLALMLAVGLVFCLGMLGLARLWAVWVSPPKPGLT
                     KGAAYECGLAPQGDVGIRFQSQYYLYAIIFLVFDVETVFLLPFAVSFTGLAPGAFVAI
                     MVFLLLLVEGLVWAWGKGLLRWR"
     gene            complement(15748..16017)
                     /locus_tag="PKK20_01640"
     CDS             complement(15748..16017)
                     /locus_tag="PKK20_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98624.1"
                     /translation="MSDVAARKRKMALLLGLGLDSDGHKRITTGPNFALIGGSEATHE
                     HMTEKALKINEKLAERGKRLEDVSAEEFDDIAHDVGLRRKSSEQN"
BASE COUNT         3408 a         5831 c         4619 g         2274 t
ORIGIN      
        1 aagcgatctg aaccggcagg gctagaccga acgcgagcca ctggaaccat cgggtcatcc
       61 ccagacccaa cacccactcc ccaaccatca gcaccaaggt caccgccccg cccaccacga
      121 cgttgaaccg ccaacccgcc agcggagacc agacctcgat gccgtggctc gcgcggtcgc
      181 ccgatgttcc cggcacatcc acgttttcag cgtcaagagc cgtcgcgcca aacccggctc
      241 gttggacggc ggcgaccacg gccttatcgt ccgcggtccg gcccggctcc caccacactg
      301 tcgcccgggc cgccgtcaga tccacgactg cgttggccac acctgcaacg ccctgaatcg
      361 cctgggttgc gttgcgcgcg caaccctggc acgtcatgcc tgtgattgag aaaccggacc
      421 tcgtccgtat ggcatcggta tccacagaaa ccaataaagc cgcaaccgcg ccattccgca
      481 aaccctgcgg atcacgatgt ccaaaagtcc cggtccttgt caccggcgtt tctaatatct
      541 taagggctga ccctgtcggg tacgattcca atgtgcagca tgcttgggat cctctagact
      601 tccgcgcatg aacctcgaag accaccttgg cgatatcctc cgcaaagggc gcttgcatgc
      661 cggcatcagc ctcgaaccgg ccgccgccgc cgccagtctc acgatccccg aactcaaaac
      721 actcgaagaa agcggcgccg ccagcagacc ttataacctc gaggcgcttg ccgccctggt
      781 cggactcgat gcgcctaaac tgcgggccat tgccgagggc tggaatccgc ggcctgtaaa
      841 cctcgacaca tggcgccagt tcagggtcat caccaccgca gcggagatga gcgtcaacgc
      901 ctacctcgcc tgggatgaag tgacgcgcga cgccgccctg ttcgacaccg gcttcgatcc
      961 ggctccggtc tttgccctga tccaggagca cggtctcgag atgcgccacc ttttcatcac
     1021 gcacggccac gtcgaccatg tcgccgccct gccggccatt cgcgcacgct ttcccaaaat
     1081 gcgcctgcac tcgagttcac ggaccgcatc ggtcgatcaa cggaaccgcc cggacgagtg
     1141 ccttcaactt ggcagcctgc gcatcacgca tcgcaacacg cccggccatg ccgaggacgg
     1201 tgtgacttac gtcattggca catggccgga cgacgcgccg cacgtggcga ttgtgggcga
     1261 cgcactgttc gccgggtcaa tgggccgggc gaccggccag gcgcttctcg ccaaacagag
     1321 aatccgcgag tgcatcctca ccctgccgcc cgccacgctc ctctgtcccg gccacggccc
     1381 gctcacgacc gtggcggagg agagggcgca caatccgttc ttctgactga gatcgagagc
     1441 cgccttagcc cgggtgtttc acaaagcttc gcggagtccg acttccggac atcaaccgtc
     1501 cctggcggcc agcaggtaga tccagttgag gaccagggcc gccaggccaa tccagtacca
     1561 gggccaagac gcccggcccc gcagccacag accccagcgc gtccccaacc accgatccgc
     1621 cccccacgcg acaaacaggg cgaggaccgc cgcgcccaac gcgcacgcca gagggttgag
     1681 ccggatcgca gtcgcaaggt ccagtccggc ggcagcggac gccgcgcgcg tggcgccgca
     1741 gaaggcgcag ggcaatccgg tgatcgtctt gaaccagcaa tggggcagag ggagccgcca
     1801 gagttcggca aaacgcgcgc cgacaagcag gcccaggagc ccaagaaaca gccatgccgg
     1861 ccaggggcca gtccagcctt ccggctcaga gtcggggcgg gattggagaa tggggggcgt
     1921 cattggattt cggcgccggc gaggggcgcg cggctcggcg atgcatgcgg atgctgctgg
     1981 ccagggccca gagccacgca gacgcaaaga gccccaaccc aagcaccgcc accatcagac
     2041 cccgggtgaa cccttccggc atctctccgg tgtcaaacca gacgcgcaga tgcgccacca
     2101 ggccgcccag gcttagtgca aagccgctga gcgcacacgc ggcctggaca taccccacct
     2161 tgcagccgcc ggcgatcgtg cccaggccgg gaagagcggc cgcgttcgtg atcgtgcagg
     2221 cccaggccgt gggcttatcg atcggctcct gcccggcgcg gtccgaagag gtcttcatac
     2281 gcttggagca acgccgcata cccaaacggc atgctcaaca gaacaacgag ttgttcctgc
     2341 aaacccaggg tcatcatgaa ctcgccgagt tgagatttct tgtcctgaat ggcatcgagt
     2401 gtccacgtgc ccggcggccc caggacatcg gtcagaaacg tcatcatccg caggccaccg
     2461 tacacttcga aaaccagtag cggcaggaac gccacgcaca tcaacagcgc cagattgaaa
     2521 aaatgacgct gcgcggtgcg ccggctcagt tccagcgccg gccagaaaac cagaccgcga
     2581 tcggcagcca gcgggagggc gaacgcccag atcaccttga ggatcagacc ggggaccaga
     2641 cagaagagca ggccgatttg gctgaacacc tgagtcacga tcgcgatgag catgagcggg
     2701 acaaaccgcg ggccaaagca ggtgaagaga gtgccaagag acgccggttg gccccgcatc
     2761 aagcgcaggt aaagcagcat caacccgcca taaagcgcgc cgctcaggat aaggccgagc
     2821 acgcccccga tgcatgtggc catcgcgctg agggtcagaa tggcccaaac cagcgcgcag
     2881 cccgccgaca ccagcaggaa atgccggcgc aagaacaccc acccgcgccc caaacaatcg
     2941 ccggcactga gggtcgccgc cggcacggca ttccacgcct cgattgtccc gatagttcca
     3001 tgcgtcgacg attcggatcg cgtcgggccg gcagccgcgt cgggatacgc ccggcgcaaa
     3061 acctccgcga actcaggcac tgtggacaga ggctgccact ccgaactgcc ttcgacctga
     3121 accatcgtct ggccattagc ccgatgatcc agaatccata cgctcaactc ctccacggtc
     3181 gccggaccgt actcgcggcc gtcgccaccg atcatcttga acatgacggc cagtctgttg
     3241 gcaatccgca cgcagcgtca agcgccggtc tgaatcgcct ccaccggcgg cttgagacgt
     3301 cttgctgccg ttgcttcggg cgtggcggat cagaagccgt ttccgccagc aattgcacgc
     3361 gccaggggac cgagcagcaa ctcaggcaat gcccccaaca ggaagacgcc gaccgccgcc
     3421 gcgatcagca caaggcggtc agtccacggc acgacaatcc gcgaggcctc ttcgtcgccc
     3481 gcgacgacat acgcctgttt caggacgcgc aagtaatagt acaacgacac tgtgctcatc
     3541 gcgatcgcga tgccgaccag ccacaaaagg cccaggcccg cggggtcggc gcccatggcc
     3601 gccgtgaaca aaccgaactt cccgaagaac ccagccaggg gtggaatgcc ggcaagcgac
     3661 aacaagaaga ccatcagaca caaggccagc agcggcgacc gccggctgag cccggccagc
     3721 gcgtcgattc gtccattgcc caatacccgc tcgatcaccg acgccacacc gaatgctccc
     3781 acggtcgtca gcccgtacgt cgccacataa tacaggaccg cggccagcgc cgcggaacgc
     3841 tgcgaaggat agcccatcgc catcagcccg agcaggacgt agcccgcatg cgcaatggcc
     3901 gaataggcca aaagccgccg cagatgcccc tgcgcaatcg ccgtgagatt gcccaacacc
     3961 atcgagccca cggccaaaac cgccagcacc ggcagccaac ccgcgcgcaa ctgtccccac
     4021 gcgccgctgc cttccacacc cgcgaatccc agcacggcga tcttggccag cacgaaaaag
     4081 ctcgctacct tcgatcccga cgcgatcagg gcggcggccg ggacgggtgc gccttcgtag
     4141 gcgtcgggcg cccacaaatg aaacggcacc acggccacct tgaatccgaa tcccatgatc
     4201 accatcacca cggccacggc gagcagcgga tcgctgcgat gcgcccgcag gccggccgcg
     4261 atcccggtca ggttggtcgc tcccgacaac ccgtaaacca ggctcaatcc gaacagggtg
     4321 aatgcggcgg ccacgctgcc gaacagaaaa tacttcagcg ccgcctcgct ggcatggcgc
     4381 gttcgtttgt ccagcgccgc cagcacgtag agcgagaggc tggtcagttc gagcgacacg
     4441 aagatcatca gaatctcttc ggtgctcacc aggaacatca ttcccaccgt ggcgagcagc
     4501 acgagcgcga aatactcgcc cacatgctcg gtgaactccg cctgcgccga gatgcccgcg
     4561 gtgaacagcg tcagcaccac caggaagacc ttgacccatt gggtcaggga atcgaccacc
     4621 agcaccccgt ccagaaaccg cagatgcgcc ccgctcgatg cgagaatccc caacgtcacc
     4681 agacagccaa gtcccgccac cgcgcaggcc gtcagaaacc gctgccgcag gggcgccgac
     4741 cgcatgagcc ccatgtcgac aaacagcacc gccacagccg ccagcacgag cgctgtctcc
     4801 ggcgccaacg ccgccagcaa atcgctgtaa cccacgcctg tcacgatcca cctcccctct
     4861 cgaggccctg gatcaaggac gtcacgctcg gcgcaatgaa gtccagtagc agccgcggat
     4921 aaagtccgat cgccaacgtc gcccccaaga gcagaatcgc ccccgcccgc tcgggaagcg
     4981 aaatgggctc gatcgcctct cccgccggtt cgccgcgcgc gtcacccgcc gaatcggcga
     5041 agaaggcgcg ctgaagcgcc cgcaacagat acgccacacc cagcacaatc cccacgcccg
     5101 ccgccaggcc ggcggtcggg aacgcccgcc acgacccgat caaaacctgc aattcggcca
     5161 cgaacccgct gaaaccgggc agccccatcg aggccatgcc ggccaacaca aacgtgacgg
     5221 ctgcgaacgg catcgctcgg gccaggcgca tcccttcgag ttcgtccaac tgccgcgtat
     5281 gcgtccggtc gtacaccatc cggcccacaa cggcgaagag caggcccgcg atgacgccgt
     5341 gcgagaacat ctgcagcacg gcgccgctga gccccacctc gttcagcgtg gccagcccca
     5401 gcaggacaaa tcccatgtgg ctcacactcg aatagccgat cacaaacttg aaatccttct
     5461 ggaccaacgc cgtcatcgca cccgccacga tgcccacgac agccaacacc gcgatctccg
     5521 tctgccagtg cctcagaccc gcggggaaca gggtcatggc aacccgcaga cagccgtacg
     5581 cccccagctt catcacaacc cccgccaaaa gcatcgacgc cccggtcggg gcggccacgt
     5641 gaccggtcgg cgcccaggtg tgaaacggcc acagacccgc cagaatccca aagcccacaa
     5701 agaccagcgg caaagcccac cgctgaaagc tctccggata gtcgacctgc gccagagcag
     5761 tcagatcgaa tgtcggtacg cccgtctggg atcgcgccac gacaaacgtc ccgatcaggc
     5821 cgatcaggac cagcgcgctt ccggcgaatg agtagagcgc cagcttcatc gcgccgtatt
     5881 ccttgcgggt cgaaccccag atcgcgatca ggaagtactt cgggatgatc gcgatctcgt
     5941 agaagacgaa caggacaaac gcgtcgaagc tcaggaacac tccgtacact ccgccaatca
     6001 gggccatgta gaacgcgaaa aactccttcg cgcgccccgt gatgttccac gagaagagaa
     6061 tccccgccac cgccgccacc gccgtcagaa ggatcagcgt gacgctgatg ccgtcgacgg
     6121 cgaggtgata atcgatgccc agggacggaa tccaactcgt ccttgcgatg gtctgcagac
     6181 cgcttcccgc ctggtattgg aatgccccgc cgaatgccgc aatcgccccg gccagagccg
     6241 tgccgagcgc cacccaccgc gcccgccccc cctggtcccg cggcaggagc atcaggaccg
     6301 ccacgcccgc aaatgaaatg tagattgtcc aggcgaacat gatcagcaag gccccaggcg
     6361 ctcgacccag aggagcacgg tacggtgaat cacgccgatg accaactgcg ggtaaatccc
     6421 cagcacgaac atcagcgccg tggcgggcgc aaccatccag atctccgccc ggctcaggtc
     6481 ggggaaggcc gcccaccgcg ccggcaacgg cccgtgaaaa acccgttcga ggaacgtcag
     6541 caggaacacc gcggtgatga gaagcccagc cgtcgaaagc gcggcgaccc acggcgccag
     6601 ggcaaatgtg cccttgaaaa tcaggaactc gcccacgaat ccgttcaggc ccggcaaccc
     6661 cagcgatcca aacgccgcca cccccatcag accggcgagc acgggcgccg gcttccgcag
     6721 cccgccgaag tcgtccagtc cgcgcaggcc gccggcccgc cgctcgatca gcgccacgaa
     6781 accgaagagc atcgcggcgg tcagcccatg attgaacatc tggagcagaa cgccgttcag
     6841 ggccgccgct ttctcgaccg cccagcgcgc ctcttctccc gtcacccgca gcgcggcgaa
     6901 cattcccagc agacagtagc cgaggtggtt caccgacgag taggccagca tccgcttcac
     6961 atcccgctgc gcaaacgccg ccgccgccga gcagacaatc gtcagcacgg ccagccccaa
     7021 gagcggcgtc tcccacgcgc gcatctggtc cggaaagatc ggcagcagaa tccgcaggaa
     7081 accgtacacg cccatcttcg acagcgcccc cgtcagaagc atcgtcgtgc ccgttggggc
     7141 cgcggcgtac gtcgagggca gccacgtgtg gaacggcagc accggaacct ttaccgcaaa
     7201 tcccaggaac gccccgaaaa agacggcttg ggccagtgcg tccgccgaca tcccggacca
     7261 gcccaggtgg gcgtgcagaa gcgccgccgt ctgatgggtc cgacccaagt ccgcgagttc
     7321 gaggaaatcg aatttgcccg tcgcgaggaa cagcgcgaga aaggccagca gcagacccac
     7381 gctgcccgcc atggtgtaaa tgaagaactg cgtcgccgcc gacccgcggt ccggaccgcc
     7441 ccagagcctg accaggaaga acgccgggat caggctcaat tcccaaaaca ggaaccagtg
     7501 gaagaaattc agcgcggtga acgccccgaa cagcccggct tcgagccaaa gcaccagcgc
     7561 gaagtagaga gggacgcgtt cctcgatgcg ccacgacacc aggagcgcca gcggcaccag
     7621 cagcgccgcc agcatcacca tcagcaatcc catgccgtcc acgcccacaa agtactcgac
     7681 ggccagcgtc gggacccacg cccgtcgctc gacatactgc aacgcagtcg aatcccggtc
     7741 gaatcccgac cagagcgccc ccgccacagc cagggctagc aagctcacgc caaggcccag
     7801 ccgccgggcg agtgtctttt gcccggccgg cagggcggct acgaccaacc cgcccaccag
     7861 cgggatcaat gtcaggagcg tgatggagcc aaacgggttc atcatgaatc gccccaaatg
     7921 aacaacagca acagcaccac cagcgccatg cccagcgcgc gcaaatagtc gtgcacctgc
     7981 ccgctctgaa agcgcgagcc ggcctgcccg ccgcgggcaa tctgccgaca ggcccggtcg
     8041 aatcccaggt tgatgccgta gtcgtccagt gcccgcgtca gccaggcgca cccgacgaca
     8101 accgcgctga tggcgtaaac gaccccgccc cagacccagc ggtcgaacgt atcgcaggtc
     8161 caggcccagg ccgcgtgcca gcgcacaacc gtgcgggcat aaaactcatc cacatagtac
     8221 ttgccccgga gcacggcgaa gaaggtcggt tgataccgct cgatggcgtc cggggcctcc
     8281 gggctcgtga tcggctgccg cccataccag taccagccca gcccgaaacc ggtcagcaca
     8341 atcagcgccg acagggcgat catcggccag tgctcccggg acagcagtgc gctcgcgtga
     8401 aactcgaccg attcgcccgt caaatagccc tgaaaccatg gccagaccgg cgtcccgaca
     8461 aatcccaaca ccaccgcgaa taccgccagc acaatcaaag gtgccgtcat cacagccgga
     8521 ctctcgtgcg gcgtgtcgcg ccgcgatgca tgggccgtcg cggcccgccc ggcgccgccc
     8581 cggtacgccc cgccaaacac ataacagacc tggcgcgtca tgtagaacgc cgtcagaagc
     8641 gccccgcaca aacccagata gaacggcgcc gccgacaccg tccagccgtg cgcggcgtgc
     8701 aggatggcgt ccttgctcca gaatcccgag aagaacaagg gcacgccggc cagagccatc
     8761 atgccgacgc cataggttgc gaacgtgacc ggcatccagc ggcgcagtcc gcccatgcgc
     8821 cggatgtcct gttcctcgtg gacgccgtga atgaccgagc cggccccgag gaacagcagc
     8881 gccttgaaaa acgcgtgcgt gatcaggtga aacatgccca ccgccacgcc ccccacgccc
     8941 aatcccatca tcatgaagcc gagctgcgag acggtcgaat aggccagaat ccgcttgatg
     9001 tcggtctgag ccaccgcaat cgacgccgca aacaccgccg tcaacgcccc cacccaggtc
     9061 accgcctgca gcgccgttgt ggcctccgcc gccccatccg gatgcaccgc catcaacgga
     9121 taaacgcgcg ccacgagaaa cacgccggcc gctaccatcg tcgccgcatg gatcaaggcg
     9181 ctcaccggcg tgggcccctc catcgcgtcc ggcagccaga catgcagcgg gagttgtccg
     9241 gacttgccga tcgccccgca gaaaagcagc aggctgatcg ccgtcgccac ccccagcccg
     9301 cccgtggtcg tctgggtgac caaccgggtc agggccgaat gctcgaggca gcccgcgccg
     9361 tcgtcgtaga actgcagcgt gcccgtttgg gcgtaaagcc agacgatcgc gagcagaaag
     9421 ccgatgtcgc caacccgggt ggtcagaaac gccttcttcg ccgcggcggc ggcgctcggt
     9481 ttgtggaacc agaatccaat cagtaaatac gatgtgagcc ccaccagttc ccagcaaatg
     9541 aacaggagca gcaggttgtt cgcaatcacc agaccgagca tcgccgcggc gaacagcgcc
     9601 aaaaagctga agaagcgcgt gaagttctcg tcgtgcgcca tgtagcccac gctgtagacg
     9661 aagatcagca gccccaccaa tgtcaccatg accagcatcg acgccgccag cgggtccagc
     9721 acccagccca actcgagatt cgtcggcccc agcgcaaacc aaacaaacct ggatgtgtcg
     9781 tgcgccggcc cgccatgccc ctggctcgcc ccgcccaggg tcgtccagaa ggcgatgaac
     9841 gacaggatca gactgagccc gatcgaaccg acggccagac cggcggccag cgtctttcgc
     9901 cgctgccgca gcagcgcgat cacccccgcc gcagccagcg gaagagccgg gatcaaccag
     9961 agataaggcg tgatggcgtt caccctttca tttcctcgat ttgatccagg ttggtcgtcc
    10021 ggtaatgccg gtacacggcg atgaccagag ccaggccaac cgccgcctca gcggctgcaa
    10081 tcgcgatcgc gaacagcacg aacatcacgc ccgtgagcgc ctccgggtgc ggaccatgcc
    10141 gccagaaggc aatgaaattg agattcgccg cattcagcat cagctcgatc ccgatcaaga
    10201 ccacgattgc attgcgccgc gtgagcacgc cggccagccc aatcccgaac agaagggccg
    10261 ccagaaccag atagcgcgcg agttctccca tagatcactc ctcgagccgc cccgcgaccg
    10321 atccctgctc cccgggcttc gccggccgcc cagggccgtt ttccctcagc gccacgagcg
    10381 ccgccccgat cagcgccacg gtgagaagaa cccccatcgc ttcgagcgcc acgacgtatc
    10441 gcgtcatgag caaatccccg atcgccttca cggtcggcgg cggcgcagag ggtggcgcgc
    10501 cgacgcccag accgctcccc agaatggccg agacgacggc gcccgacacc agcagcgcga
    10561 tggccaagcc aagcgcccat ggcctgaacg acagcgcctc gtgcgtggcc tcgaaattcc
    10621 gcgtgagcaa cagcgcgacg acaatgagaa tcgccacggc gccgacgtag accagcaact
    10681 gggcgaagcc gacaaactcg gcgcgcagtt gcaggaacgc caccgccagg ctcgcgaacg
    10741 cgaccactcc gcacagggcg cagtgcacca cgctgcgcgc ggccatcgcg cccgccgcgc
    10801 cggccagaat cgccgctgct gtgatgagaa aggcgatgtc catcgtcagg tccagctcag
    10861 gcagccatgc ggtaggtccg cggaccgatg tgttgtccgg caaacagccg ccgcccaagc
    10921 aggacatacg gcacacccac caggctcccg cacaggagcc agcgggccag cccggcgccg
    10981 acgaaagtcc acggcgccgt gtagtgccag agcgcggccg acaccgcgtt caggagcacc
    11041 agcggcagga ggaatttcca ggccagattc atcagatgat cggcccgcag acgaggcagc
    11101 gtcccgcgca gccagatgaa cagcgcgaca aagccaccca atttcaggaa gaaccacatg
    11161 tacgacggca cccactcgag ccagatgacc ggggcgcgcc acccgcccaa aaacagcgtg
    11221 atccccagcg cgctcaccgc gaacattccg aggtactccc caatgaaaaa cagggcgtac
    11281 ttgattcctg agtactcgac caggtgcccg gcaatgatct ccgattcccc ctccggcaga
    11341 tcgaacgggg cgcgattcga ctcggccgtc gccgccacgg tgaaaagcag cagaccggcc
    11401 aggccccacg gtgtgaacac atgccagcgc gccagccatc cgccagaatg cccggcctgc
    11461 gcgtcggtga tatccaccag agacagcgag cccgcgagca tgacgacggg cagcgtcgag
    11521 agaatcagcg gcaactcgta actgatcatc tgggcaatcg cccgcatgcc gcccaggagc
    11581 gagtacttgt tgcggctgga ccaccccgcc atgaacaccg ccagttcgat gcacgccccg
    11641 accgcaaaga agaacaacag gcccgcgttg aaatcaaccg ccgccatgtt gcgtccgatg
    11701 ggtatgaccg cgtaggccat gagcaccggc accaccatca ccaccggcgc cacaaaatgc
    11761 acgacacggt ccgccccgct cggcacaagg tcctccttgg tcagcatctt gatcaaatcc
    11821 gctatcggct ggaggagccc ccacggaccc acccggttgg gcccataccg gttctggatg
    11881 cgtcccagtc ccttgcgctc gatcagaaca gtcagcgcaa acagggtcat gaacacaccg
    11941 agaatcgccg ccgccgaaat cgccaacgac acccaagact gccaagcggg cggcaactgg
    12001 cccaccagcc agtgcttcag gctcacaaaa agctgatcga gcgcctcaaa catcgtggct
    12061 gccgaaggcg gcacgcccgc aatcaagggc gttcgctccg gaaatgggaa tggtttggct
    12121 ggccacgcgc atcttaaggt ctgtctgaat cgccaggttt tgccccggac gtcgttcccg
    12181 ccgccggcgc cggccctgcc gccggtttgg ccttcgtctc ggcgtggccc ggtcccgccg
    12241 caagccgggc gttcacctcc cccgcctcga cgggatggag cccgagatag tacgaggcgg
    12301 gtctggcgag atccatcttg tgcaggagaa gggacccgaa ccggtcggcc gtgctcagtt
    12361 cgaattccgt attcatcttg atcgcgtcga aggggcaaac ctcgacacag atctggcagc
    12421 tcatgcacac cgaaatgtcg aggtcgaaga tcttcggacg cctcagtggc cggcccttct
    12481 cgtcccgatc aagaacgata tagatgcact gcggcgggca ctccttctcg cagatcttgc
    12541 aggccacgca ccgcaggccc ttctcggggt cctcgccgtc gtagacgagc atcgggaaac
    12601 tgcgcgcctt ctccggcaac gcgcggcgtt cgtccgggta ctgaatcgtg accagccggg
    12661 ctgggtcgta atagctgccg acgaaattcc gggctgtctc gacgaggccc ttgagaatgc
    12721 ccttgcccag attcatcggt ccacctcccc catcacgatg tcgaccgcgc ccaggatggc
    12781 cacggtgtcc tgaagggtcg ctccccggca catgtcctcg agaatcgtca ggtttatgaa
    12841 gctgggcgga cgaatccgat agcggtaggg attggccgat ccgtcgctga tcaagtagaa
    12901 gccgagttcg cctttcggcg cctcgatgcg cccgtacacc tcgcccgccg gcggcttgaa
    12961 cccgcgcagc cgcgccttcg ggttgatgat cgggcctgca ggcaccgccc gcagcgcctg
    13021 ctgcagaatg cgcaccgatt cgcgcatctc gagcatccgg atcatgtacc gatcgtacac
    13081 gtcgccgtgc gcccccagcg gcacgccaaa ctcgaatcgg tcgtagaccc cgtaccgatc
    13141 caccttccgc agatcgtagt tcacgccgct agcccgaagc atcggtcccg tcatcccggc
    13201 ctgcaccgcc tgttcggctg aaagcacgcc gattccctgc gtgcgcgcca tcaggatttc
    13261 attggtcgac aggaggttct cgaactcgtc caggaaccgg ggcaaggcct ccaccacgtc
    13321 ccgacaggcc tcgatccagc cgctcgggag atcgcaccgg catccgccaa aacgcatgta
    13381 gttgcacatc atgcgcgagc ccgtcagggc ctcgatcaac tcgagaatct tctcgcgctc
    13441 gcgaaaggcg tacatgatcg gcgtgcccat cgaccccagt tcctgcaaga gaaacccaat
    13501 caggcacgcg tggttgagca cccgcgtcaa ttcggccgta attacccgaa tgtgctccgc
    13561 ccgctccggc acctcgatcc cggccaggcg ctcgacggcc agggcgtagg cccagttatt
    13621 cgtcagcgaa cagaagtaat cgagccgatc cgtgtacggc atcgccgcca aatacgcccc
    13681 cccctcgccc agcttttcgt ggttccggtg cagatagccg aacaccggct tgagccggac
    13741 cacccgttcc ccgtccagca cgacatccat ccgaaacacc ccgtgcgtcg atggatggtg
    13801 cggccccacc gccacttcga gcagcgtccc ctcggagtcg cccagtgcga tcggatgcgg
    13861 cttcgcctcg gacgccgtcc gcggcgcccc ctccgggcgc ggcaagaccg ccgcgtgcga
    13921 cggactttcc ggcaccagat cgccggtgga cagcgcctcg atcagcacgt cttcggggat
    13981 ccggcggttc atgcgtcggc ctcgatcgga taatggcgcc gcgccttctc cttcaaatcg
    14041 tcgtgcggcg tcggctcgta ttcgtaatcg tccggctcca cgtagtcctt ccgcatcgga
    14101 taatcccgga actcatccca catcaggatt cgcctcaggt ccggatgccc ctcgaaaaca
    14161 atgccgaaaa gatcgtacgc ctcgcgttcc tggaattccg cgctccgcca caccggcgtc
    14221 agcgaggtta cgcgcgcctg gtccacccgg ttgaccgtgc gctggcgcag gatcaccggc
    14281 ccgtgcttca accgcatcga gtagaggtga tacacggctt caagataccc ggggacgcgc
    14341 cgctcccggc attcgtcgaa caccctctcc cgcccgtcga tcacccgcgt cacacgcgca
    14401 tgctcctcca caacccggtc cagccaatcg accccgctga cattcgagca gtagtcgagc
    14461 gccaaccgcg gatcgtcccg caagaatcga gccgcctcga tcgcgcgggc cggaggcaca
    14521 accagtgacg cctggcccgg caccgccccg ttgcactgaa tctcaagccc gagaccgggg
    14581 attgcggctt cgaggcggga cttgatttgt tcgagtgtct ccacgggctc ggtttgggcg
    14641 cgcggcgcct ggtcagtgaa tgtgatctac tcgtcgcgaa tgatcggctt cggacgccac
    14701 aggtcggtgt tcttggccgg gaccaggtcg taagggccat actcaggcac cgggaactcg
    14761 ccgggagcct cctcctgaaa cgcccgggcg cgggagggtc cggtcagttt ctgcgacgca
    14821 atctctttct ggagggcgat gagcccgtgc agcaacgctt ccgggcaagg aggacaccct
    14881 gggatatgga cgtcgaccgg cacataccgg tcgatgccct taagaacgtt gtagccgcgc
    14941 ttgaacggcc cgcccgaaat ggcgcacgcc cccatcgcga tcacgtactt cggttccggc
    15001 atctggttgt acagccgcac cacctgcggc gccatcttct tggtcactgt ccccgccaca
    15061 atcatcaggt ccgcctgccg cggcgagggg cggaacacct ccgcgccgaa ccgcgcgatg
    15121 tcgaaccgcg ccatcgaggc tgcaatcatc tcgatggcac agcaggccaa accgaagttg
    15181 agaggccaaa tcgagttgcg gcacccccag ttgtaaaggt cctggattgt tgtggcgaag
    15241 atgccctgtc tgctcaactc gctgcgcaat ccttcatcca tggtcgacat tctggagtgt
    15301 tcggcttcgc gctggtcaca aaaccatgca atacgcgggc tagcgccacc gcagcaaccc
    15361 cttcccccat gcccacacca gcccctcgac cagcaacaac agaaaaacca tgatcgccac
    15421 aaacgccccc ggcgccaaac cggtgaacga caccgcaaac ggcaggagga agaccgtctc
    15481 gacgtcgaac accagaaaga tgatcgcgta gagatagtac tgggactgaa accggatgcc
    15541 gacatcgccc tgcggcgcca ggccgcattc gtaggccgca cccttcgtga gacctggctt
    15601 cgggggggag acccacaccg cccacaatct cgccagaccc agcatcccca ggcaaaacac
    15661 caaacccacc gccaacatca gagcgagaaa caggtaaggg tggggtgcgg acggcatgcg
    15721 cgacactaaa tcccgattcc aattgattca gttttgctcg gatgactttc gccggagacc
    15781 gacgtcgtgt gcaatgtcgt caaactcctc cgcgctcacg tcttcgagac gcttgccgcg
    15841 ctcggccagt ttctcgttga tcttcaacgc cttctccgtc atgtgctcat gcgtcgcctc
    15901 cgagccgccg atgagcgcaa agttcggccc ggtcgtaatg cgtttgtggc cgtccgaatc
    15961 caggcccagg cccagcaaca gggccatctt gcgctttctg gctgcgacat ctgacatgcc
    16021 gccacgctac ggccacagcc cctcccttcc aagcaaaaaa ggcgccgccc gcccgggacg
    16081 tgacgcgcaa tggccctacg gacgggtttg gccgccgtct gtcgcctccg tc
//
LOCUS       DAOQRO010000021        15951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2750_length_15951_cov_4.652932, whole genome shotgun sequence.
ACCESSION   DAOQRO010000021 DAOQRO010000000
VERSION     DAOQRO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2750_length_15951_cov_4.652932"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PKK20_01645"
     CDS             complement(<1..891)
                     /locus_tag="PKK20_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNU98625.1"
                     /translation="MGSLFAAGASAYWPLVVLAICMALVIFLITVARLHAFIALILAA
                     IAAGLLAPAGSLPGETPGAGHWVHAVELTTSEFGLTAGKIAVVIGLASVISMCLMESG
                     AADKVVRRFLAIFGERRAGAAILISGYILSIPIFFDTFFMLLLPLARALCLRTGKDYL
                     LYVMAICCSGVVTHSMCAPHPGPLAMAEVLHVDLGLSIFVGVAVGIVPVAASWTVAKW
                     INARMTVPMRDTPGVALDDLRAIVNQSEDELPSFFWSILPVILPILLISLASAFAAGP
                     LPADQRPIWHAMIAFAGNRNV"
     gene            complement(904..2109)
                     /locus_tag="PKK20_01650"
     CDS             complement(904..2109)
                     /locus_tag="PKK20_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98626.1"
                     /translation="MRYRVTLVLLLLSLLANFVIATAWRRARKPSAVYHLEPPPRRAV
                     VTNILRPIRTNLVFQPRLLSWRDIESDDYPTYIRNLESIGCPRETIRDIVVADVNELF
                     ARRRATEVVSPNHQWWLPEPDPEVVQEAIEEKGALEAERRDLLTRLLGPHWDVTGTAT
                     EIPGSAITLDGPLLGELSAETKQTLRDIEFRGREKLRLHAQSMREQGREMDPAESARV
                     ARELREEMAKVLTPAQLEEYLLRYSENADRLRRTLAGFDATAEEFRSLFRATDSIDLE
                     LAALAGATDPQSAKRRQELEHQRAEATRSALGDARNTYYQLSQDPVFQQARQAAERLG
                     APAETVLPLYQIAQETDRERRRILNDLTLTPDQQTKELTAVDQARMDFLRALLGEDRF
                     RRFESGNAR"
     gene            2219..3070
                     /locus_tag="PKK20_01655"
     CDS             2219..3070
                     /locus_tag="PKK20_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNU98627.1"
                     /translation="MSERMFTLGHSPDPDDAFMFYGLAKGLIPTPGYRFEHILEDIQT
                     LNERATRGELDISAISIHAYAYVADTYALLPSGASMGDGYGPMLVAKRPVGRNDIAAC
                     RIAVPGTMTSAFLALQLWLERRADQLSCVVVPFDRIFAAVKEDRADVGLIIHEGQLTW
                     DKEGLVVCQDLGAWWKRENTGLPLPLGGNVIHKRIPAEDRCRIADIVRTSIQFSLDHR
                     AEAVGHALQYARDMGRDLADQFVGMYVNHWTLDYGDPGREAIRRFLAAAQARGLIPKS
                     PPLEFVN"
     gene            3089..3472
                     /locus_tag="PKK20_01660"
     CDS             3089..3472
                     /locus_tag="PKK20_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNU98628.1"
                     /translation="MKNHTIIQPVGAPAPVGPYSHAVRVGPLLFCSGQIPLDPATGVL
                     VPGDVKAQTDRAIENIRIILASENLTLANVVKTTVYLLDMADFAAMNEVYGRHFSGDC
                     PARSAVQVAALPRGARVEIEAIAAY"
     gene            3760..4791
                     /locus_tag="PKK20_01665"
     CDS             3760..4791
                     /locus_tag="PKK20_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNU98629.1"
                     /translation="MVTNAPVAPLRRRRFERGGRSRPPLERMLRIHQAIQSGRHPNAT
                     TLARELEVSAKSIVRDLQFMRDRLDLPIEYDDRRWGYFYTQEVSAFPNLQITEGELFA
                     LLVAEKAVRQYRGTNFERPLVSALKKLADALPETVSLQWSDWDQTISFRTSAEPVLNL
                     EVFDALARAVARREQIELAYRKPGCSEADRRVVDPYHLANINGEWFLFAFDHLRRDLR
                     TFVPARIQSVRPTGRRFVRPRGFSVDRQLRGSFGVHSAEGRFDVVLRFTPRVADYIRE
                     KRWHPSQVLADRPNGGVELRLKLSSLIEIRRWILGWGGEATVVRPPELADAVCQAAQA
                     ILDTRIADR"
     gene            complement(4871..7762)
                     /locus_tag="PKK20_01670"
     CDS             complement(4871..7762)
                     /locus_tag="PKK20_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNU98630.1"
                     /translation="MSASSEADNSRRETEQAFWDSEMLYHSLVDCLPQSIFRKDTEGR
                     FTFVNQRFCRTVGRPPEEILGKTDADFYPSELAAKYRDDDERVMDTLTVLDAIEENVR
                     PNGERTYVQVIKTPLHDASGKVTGVQGIFWDVTERKRMEEAIEHERDLLQALLDSIPD
                     AIYFKDRQSRFLRVSREMARMFGARDSSQVVGKTDFDFFGEAHARQAYEDEQRIIATG
                     EPIVAKKELETWPDGRQTWALTTKMPLCDRNGNIIGTIGVSKDITTLVEAERQLEKAR
                     DAAVESVRLKGEFLANMSHEIRTPMHAIIGMAGLLLETELSVEQRDGAETIRRSGELL
                     LNIINDILDFSRMEAGRMKLDHVDFDLQEVVEGTVDLLAESAHAKGVELVYCLGDALP
                     RRLRGDPGRLGQVLTNLIGNAIKFTDRGEIVLDIQSRETTETHTTIRAEIRDTGIGIV
                     PEAQSRIFGAFSQADGSTTRKYGGTGLGLAICKQLVELMGGEIGVRSQVGRGSTFWFT
                     ASFERQPESARAAEPSRDLLAGVRALVVDDHPLNRRLLVETLGRWRVHADEAANGAAA
                     LALLQSAARAGAAYDLALIDMEMPEMDGLSLAEGIRADPNTSQTRLIMLTSLRDRLDV
                     ATMRSAGIAACLVKPPREGRLYDGLVKVMAAATPSGAHEPASLAARLHSPSPSATASV
                     SLRILLAEDNLVNQRLALRQLRRLGHAADAVSNGRQVLQALERQTYDLLLLDCQMPEL
                     DGYETARAIRKQEVPISGTAGTPRRPLRIIAMTANSMEGDRERCVAAGMDDYIPKPVR
                     LPDLEAALQRASERLTADLDLPSAPSVAQPGSDEETLNLSMLRSLSELNVPGETDAVV
                     EMAELFFQSAVPLRDGLLATAAAGDLAGFARAAHSLKGSASGLGAQRLARLCAELEQS
                     AKAGDLASVQRDVGAASSEFEKVSLALAAATGLSVPAQARQPNPPATPH"
     gene            complement(7773..11450)
                     /locus_tag="PKK20_01675"
     CDS             complement(7773..11450)
                     /locus_tag="PKK20_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNU98631.1"
                     /translation="MGDILRLVKVDPRFTKTFGIEVFTGNELLVKGCLETVGGTHLWT
                     GYPGSPVAGFFDTASEIHELLAEQGIRATMANNEALSAAMVNGSQMHGLRAISVQKSV
                     GVHVAADALALGNLVGAHPEGGAVIVLGDDPWSDSTQVPADSRYIAKHLMMPVLEPSD
                     AQELKDWVNLAFTLSRESELYIGYLVTTNQADGGGTVRVRPNHAPALNLHTPFDLDTA
                     KIDFERNVLLPPRTWRKEQGLEDRYTRLWASARRHGANRILPGNDTNFEARIGFISSA
                     AAYCYLRHALAEMGVDGHFPILKVGVTYPLDPALIQEFTSELKDVFVIEERRGFLEEQ
                     VLAIVTRAWQKQSGSGPASCCRVWGKQFPHGLNPIPSTRGLNPSKLIGRLGPLLLSLP
                     ELEGRIDAARIRQELDLIREIKSVVVDIAARTPTFCPGCPHRDSASVLVEMKKQFRDS
                     RYMRRVHGSGPVDLVFHGDTGCYTMLMFEPTKDLMHNYSGMGLGGGTGAGIDPFIRNK
                     QVVFMGDSTFFHSGQIAISNSLKNGQDITYIVLDNKTTAMTGHQTTPALDQGLMGERT
                     FAQNIDAVIAGITDQTRSGATVVRVNPAERESYRELLEKVILQDGVKVVVADKECGIT
                     YHRRAAREERRILREKGFLPEKRFINITHEVCENCLECTKASGCPGLTFAQTPYGRKV
                     QIDFSWCVADGACARAMVSTPEDGPRVQPCPAFEEVTVLRRQKPPTLFERLDASSLPT
                     PTPPPIEGRWHGYLAGVGGMGIGTATAILVLAGNREGYQVRFCDKKGLAIRNGGVYSM
                     ITYAVEGAPYCSNLIPYGKADLVIGVDILEAARAIDPSTNQRVGSRHRTAFVVNTHKT
                     PTILSLLGKDDFQVKVLEDLLRRNSRSEAYLSCNVSEVSECYFGTQLYANIVMLGLAF
                     QRGLIPLRLETFEWAIRSGLGAAGSENVKAFTLGRLMAHDLDAVRQSAGLHSESATYA
                     GILEDKAEILARTRRRGKALAAAYRAMTEEAVRDLPMDDRALADFALRVYDLIQFENV
                     AYAARYIALVRRICASDSAGRDFEATKAVIWNLHKVMLIKDEVYVAHLLTSEEKHRRD
                     RAWYNVDPARGDRLVYRHLNRPQFALLGRQVAFNLRTRDWMLSLIKHQRWLRRVMPDW
                     HRQEKDFRDWYIGLLAGFDEASRTPRGYDACVRVLRLPETVTGYREIRYPKMAAAREQ
                     AALLAHEIGLVDPSKASLISV"
     gene            complement(11491..13137)
                     /locus_tag="PKK20_01680"
     CDS             complement(11491..13137)
                     /locus_tag="PKK20_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98632.1"
                     /translation="MNAWKPSRLAKWLKRAVFIAVALVTLLALAYAIESYRARRLWQG
                     YRAELEAQGETLDFRRLLPEPVPDDNNFFMTPLLRPLSDYVVDGASGAVGRRDSKACD
                     RVTEMFAWTSRLRDTNPGWQMGQFRDLAVWQRQLRGLNDGAADTAAGAPADAPPDVVA
                     DSDGTQQPVSKAAHPVLEALAARPMGKPAEDLLFALAQNQAALAEIRDAAQRPAANLK
                     ARYGEDMELLLQQLASLKAMSRPLVCSALAHLAADDPASAARDVRAALRLADALSEEP
                     LLVAALVRIALTDSAMQGLWEGLARHEWSEPQLAEFEGALRRANFVKEMAHCLRGERA
                     FAIGMIGGAAEVHEAGSRGIDASATRPYDSLPGPMKYQNQYHIARLYQEHLLPAFDTV
                     TGTIDVARLDKQSIEARLGSRTPYNLFASMMGPPVSAAARNAAKAQATVNLARVAVAI
                     ERHRLDRGALPDSLEALAPRYLNPVPPDPVNGQPLRYRRGEGDRFTLYSLGMNGQDDQ
                     AAVAVTRHHGVAGRAKVGDWVWQNHPVTNTVVVSSGSEAE"
     gene            complement(13097..13576)
                     /locus_tag="PKK20_01685"
     CDS             complement(13097..13576)
                     /locus_tag="PKK20_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98633.1"
                     /translation="MNPDEFEQRLRKEAFRPIPDEWRPELLREARLEGAAAASRAPSP
                     FHRLGEWLCDWLWPAPAAWAALAVCWVAVVILNRSALPSAVEMAEAQANARLALAYQA
                     AVRGFNALQLDDPAPAPKPPEASPPRRPEEPNQARMHRQPTSSPYECLETVSTRQMA"
     gene            complement(13573..14085)
                     /locus_tag="PKK20_01690"
     CDS             complement(13573..14085)
                     /locus_tag="PKK20_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNU98634.1"
                     /translation="MPAASDLERIYDDHAQALFAFALDLTRSEADTRDLLQEMFRRLA
                     VRPLRDANVRDARAFLLRMAHNLWVDQVRRHSTRERVRNTLATAAEPVFAPADDPDSE
                     TFRRELDAAFAELPPEQRAVVHLKLWEEMTFESISRTLDIPLNTAASRYRYGLDKLRA
                     RLRPLYAEIQ"
     gene            complement(14186..14968)
                     /locus_tag="PKK20_01695"
     CDS             complement(14186..14968)
                     /locus_tag="PKK20_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNU98635.1"
                     /translation="MSYKFTGQWVLITGASSGFGAAGAMAFGRAGARLLLGARREDRL
                     SAAALEAKAAGAAEVGYHPLDVASTASVEAFASWVREFTPRVDVLVNNAGGALGLEPV
                     VEGKDADWETMMQTNVLGVLRMTRAVLPLMLPHRAGTIINIGSIAGRTAYEGAAAYCA
                     AKAGELQITRSLRLELCGTGLRVATIDPGMAETEFSLVRFKGDIERAKKVYEGVQALR
                     SEDVAEAMVWVASRPPHVCIDEMIIKCTDQAAIHKVNRRAKA"
     gene            complement(15102..15176)
                     /locus_tag="PKK20_01700"
     tRNA            complement(15102..15176)
                     /locus_tag="PKK20_01700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15142..15144),aa:Val,seq:gac)
     gene            complement(15210..15434)
                     /locus_tag="PKK20_01705"
                     /pseudo
     CDS             complement(15210..15434)
                     /locus_tag="PKK20_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863524.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            complement(15443..15709)
                     /locus_tag="PKK20_01710"
     CDS             complement(15443..15709)
                     /locus_tag="PKK20_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU98636.1"
                     /translation="MKIEVRKPAEAELRSLDVFAWPIWSCEVSRFDWSYGDQETCYLL
                     EGRVTVEVGGESVSFGKGDLVVFPRGLSCVWDVKEPVRKHYRFE"
BASE COUNT         2850 a         5263 c         5018 g         2820 t
ORIGIN      
        1 cacgttgcgg ttgccggcga aagcgatcat tgcatgccaa atcggacgct gatccgcggg
       61 cagcggaccg gccgcgaacg ccgacgcgag actgatcagg aggatcggga gaataaccgg
      121 gagaatcgac cagaagaagg acggcagctc gtcttcactc tggttgacga tcgcgcggag
      181 atcatcgagc gccaccccgg gcgtgtcgcg catcggcacc gtcatccgcg cgttgatcca
      241 tttggccacg gtccaggacg ccgcaacggg cacaatgccg acggctacgc cgacgaagat
      301 cgagaggccc agatcgacgt ggaggacttc ggccatcgcc agcggcccgg gatgcggcgc
      361 gcacatcgaa tgcgtcacca cgccgctgca acagatggcc atgacataaa gcagatagtc
      421 tttgccggtc cggaggcaca gggcgcgcgc cagcggcagc aggagcatga agaaggtgtc
      481 gaagaagatg gggatggaca ggatataccc ggatatcagg atcgcggcgc ccgcccgacg
      541 ttcgccaaaa atagccagga aacgccgcac aaccttgtcc gccgcgccgc tctccatcag
      601 gcacatgctg atcaccgaag cgagcccgat caccacggcg atctttccgg ctgtgaggcc
      661 aaactcggat gtcgtcaact cgacggcgtg gacccaatgg ccggcgccgg gcgtctcgcc
      721 cggcaaagac ccggcgggcg cgaggagacc cgccgcaatg gcagccagga tgagggcgat
      781 gaaggcgtgc aggcgagcca cggtgatcag aaagatcacc agggccatgc agatggccag
      841 gaccaccagg ggccagtacg ctgaggcgcc ggcggcaaag agagatccca tgcagtcctc
      901 tggttagcgc gcgttgccgc tctcgaaacg acgaaaccga tcttccccca ggagcgcacg
      961 caggaaatcc atccgggcct ggtccaccgc tgtcagttcc ttggtctgct ggtcgggggt
     1021 gagcgtcagg tcgttgagga tgcggcggcg ttcccggtcc gtttcctggg cgatctggta
     1081 gagcggcagc acggtttcgg caggggcccc caaacgctcg gccgcctgcc gcgcctgctg
     1141 gaagacggga tcctggctca gttggtagta ggtgttgcgc gcgtcgccaa gggctgaacg
     1201 cgtcgcctcg gcccgctggt gttcgagttc ctggcgccgc ttggcgctct ggggatcggt
     1261 ggccccggcc agggcggcca gttcgaggtc gatgctgtcg gtcgcgcgga acaagcttcg
     1321 gaactcctcc gcggtcgcgt cgaagcccgc cagggtgcgg cggagccggt cggcgttctc
     1381 cgagtaccgc agcaggtact cttcgagctg tgcgggcgtg aggaccttgg ccatctcctc
     1441 gcgcagttcc ctggcgacac gagcggactc ggcgggatcc atctccctcc cctgttcgcg
     1501 catcgactgg gcatgcaagc gcagcttctc gcggccgcgg aactcgatgt cgcgcagggt
     1561 ctgcttagtt tcggctgaga gctccccgag cagaggccca tcgagagtga tggcgctgcc
     1621 aggaatctcc gtggctgtgc cggtgacatc ccagtgcgga cccagaaggc gggtgagcag
     1681 atcgcgccgc tcggcttcga gcgcgccctt ttcttcgatc gcctcctgga cgacctcggg
     1741 atcgggctcg ggcagccacc attggtgatt gggcgacacc acctcggtgg cgcggcgtcg
     1801 ggcgaacaac tcgttaacat cggccaccac gatatcgcga atggtttccc tggggcaccc
     1861 gatgctttcg aggtttcgga tgtaggtggg gtagtcgtcg gattcgatgt cgcgccaact
     1921 caggagccgc ggctggaaaa cgaggttcgt gcgaatgggc cggagaatat tcgtcaccac
     1981 ggcgcggcgc ggcggcggtt ccaggtgata gacggcggat ggcttgcgcg cacgccgcca
     2041 cgccgtcgca ataacgaagt tggccagcag cgacagcaac agcagaacca gggtcacccg
     2101 gtagcgcatg gggcgattct ggcgcattcg cccgcaatgg gcaaatccaa agtcgcgctg
     2161 ggctcattgt gcgcttattg gttggtcagt cgcgtgccga cccgctagtc tgtccgccat
     2221 gagcgaacgc atgttcacac tcggacactc gcccgacccg gacgacgcct tcatgttcta
     2281 cggtctggcg aaaggcctga tcccgacgcc tggctatcgc ttcgagcata tcctcgagga
     2341 catccagacg ctgaacgagc gggccacgcg cggcgaactc gacatctccg ccatcagcat
     2401 ccatgcctat gcctacgtgg cggacaccta cgcgctgttg cccagcggcg ccagcatggg
     2461 cgacggctat ggccccatgc tcgtcgccaa gcggcccgtc ggacggaacg acattgccgc
     2521 gtgccgcatc gcggtgccgg gcacaatgac cagcgccttt ctcgccctgc aactctggct
     2581 ggagcgacgc gccgaccagc tctcgtgcgt ggtcgtcccg ttcgatcgca tcttcgccgc
     2641 cgtcaaagag gatcgggccg atgtcggtct catcattcac gagggccaac tgacctggga
     2701 caaggagggg ctcgttgttt gccaggatct cggggcgtgg tggaaacgcg aaaacaccgg
     2761 cctccccctg cccctgggcg gcaacgtcat ccataaacgc atccccgcgg aggatcgctg
     2821 tcgcatcgcc gacatcgttc ggaccagcat ccagttcagt ctggatcacc gcgcggaagc
     2881 cgtcggccat gcgcttcagt acgcgcggga catgggacgc gacctcgccg atcagtttgt
     2941 cggcatgtac gtgaaccact ggacgctcga ttacggcgac ccgggccgcg aagcgatccg
     3001 ccgcttcctc gccgcggcgc aagcccgcgg tctcatcccc aaaagcccgc ccctcgaatt
     3061 cgtcaattga ctcgcatcca cacccatcat gaaaaaccac acgatcattc agcctgtcgg
     3121 cgcgccggca cccgtgggcc cctacagcca cgctgttcgg gttggtcccc tgctcttctg
     3181 ctcgggccag attccgctcg atcccgccac cggcgtcctg gtgccgggcg acgtcaaggc
     3241 gcagaccgac cgggccatcg agaacatcag gatcatcctc gccagcgaga acctgacgct
     3301 ggccaatgtg gtgaagacaa ccgtgtacct gctcgacatg gcggatttcg ccgcgatgaa
     3361 cgaggtttac ggacgccact tcagcggcga ctgcccggcg cgctcggccg tgcaggttgc
     3421 ggccctgcca cggggcgcgc gcgtcgagat cgaggcgatc gcagcatatt gatgtttccg
     3481 gcagtcccgg gcttatttcc gccattcagc gggaaaaacc tgcaattctc gatttgacaa
     3541 cgcgcagagc ggcgaatccg ggaacccggc ctacgaccgc tcgattctcc ggtgctgtaa
     3601 gccgggcgcc cgcacctggc ggcaaagcca agcacccgga atgagagccg ccaggggaac
     3661 acggcaggaa cagatcacat gaacgcgatg ccggacaagg aaaggccccg cagcgcggca
     3721 aagccgcgac caaaacaact agaacctcgc gcacgtccgg tggtgacgaa cgcgccagtc
     3781 gccccgctgc gccggcgccg cttcgaacgg ggcggtcggt cgcgtccgcc gctcgagcgg
     3841 atgctgcgga ttcaccaggc catccagtcg ggtcggcatc ccaacgcgac cacgctcgcg
     3901 cgcgaactcg aagtcagcgc caagtcgatt gtccgcgatt tgcagttcat gcgcgaccgc
     3961 ctggacctgc ccatcgagta tgacgatcgc cgctgggggt atttttacac gcaggaagtc
     4021 agcgcgttcc cgaatctgca gattaccgaa ggagaactct tcgccttgct cgtggccgag
     4081 aaagcagtcc ggcaatatcg cggcacgaat ttcgagcgtc cgctcgtgag cgccctcaag
     4141 aagctggccg acgcactgcc cgagaccgtc tcactccagt ggtcggactg ggaccagacg
     4201 atctcgttcc gcaccagtgc cgaaccggtc ctgaatctcg aggtttttga cgccctggcc
     4261 agagcagttg cgcggcgcga acagatcgag ttggcttatc ggaaaccggg ctgctccgag
     4321 gcggatcgcc gggtggtcga cccgtatcat ctggcgaata tcaacggcga gtggtttctc
     4381 ttcgcattcg atcatctgcg gcgcgacttg cggacattcg tgcccgccag gattcagtcg
     4441 gtgcgcccga cgggccggcg cttcgtgcgt ccccgaggat tctcggtcga ccggcaattg
     4501 cggggcagtt tcggcgtcca ctcggccgag ggccgcttcg atgtggtgct tcgcttcacg
     4561 cccagggtgg ccgattacat ccgcgagaag cggtggcatc cttcccaggt ccttgcagac
     4621 cgccccaacg gcggtgtcga actgcggctg aaattgtcga gtctgatcga gatacggcgc
     4681 tggattctcg gttggggggg tgaggcgacc gtggtccgtc cgccggagct ggcggatgcg
     4741 gtgtgtcagg ccgcgcaggc gatcctcgat accagaattg ctgaccggtg agtctgcgga
     4801 gatcggagat cggggtgcgt cggtacgtcg gtgcgtcggt gcgtcggtgc gtcgaaggcc
     4861 ggtctgatgt tcagtgcggg gtcgcaggcg ggttcggctg acgtgcctgc gccggcacgg
     4921 acaggccggt agccgccgcc agggccaggc tcactttttc gaactcggaa cttgccgcgc
     4981 cgacgtcccg ctgcacggag gcgaggtcgc ctgccttggc actctgttcg agttcggcgc
     5041 acaggcgcgc caatcgctgg gcgcccaaac cgctggcgct gcccttgaga ctgtgggcgg
     5101 cgcgcgcgaa gccggcgaga tcgccggccg cggcggtggc aaggaggcca tcgcggagag
     5161 ggacggcgct ctgaaagaaa agctcggcca tctcgacgac ggcgtcggtc tcgcccggca
     5221 cgttcagttc ggagaggctg cgcaacatgg agaggttgag cgtttcctcg tcggaacccg
     5281 gctgggccac cgagggagcc gagggcaggt ccaggtcagc agtcagcctt tcacttgccc
     5341 gctgcaaggc ggcttcgagg tcgggaaggc ggacgggctt ggggatgtag tcgtccatcc
     5401 cagcggcaac gcagcgttcc cgatcccctt ccatggagtt cgcggtcatc gcgatgatcc
     5461 gcaaggggcg ccggggcgtc ccggccgtgc cgctgatcgg cacctcctgt ttgcggatgg
     5521 cgcgggcggt ttcgtagcca tcgagttcgg gcatctgaca atcgaggagc aggaggtcgt
     5581 acgtttgccg ttccaaggcc tggagcacct gccgaccgtt cgagacggca tctgcggcat
     5641 gacccagccg gcggagctga cgcagggcaa ggcgctggtt gacgagattg tcctcggcca
     5701 acaggatgcg aagcgagacg gacgccgtgg ctgaggggga cggactgtgg agtcgggcgg
     5761 caagacttgc aggttcatgt gcgcctgagg gtgtggcggc ggccatcacc ttgaccaggc
     5821 cgtcgtagag acggccctcg cgcggcggct tgacgaggca cgcggcgatt cctgcgctgc
     5881 gcatggttgc cacatcgagc cggtcccgca gcgaggtcag catgatgagc cgtgtctggc
     5941 tggtgtttgg atccgcgcga atgccctcgg ccaggctcag accgtccatc tccggcatct
     6001 ccatgtcgat cagggccaaa tcgtacgcgg cccccgcgcg ggcggcgctc tggagcaggg
     6061 ccagggccgc ggcgccgttt gcggcttcat cggcgtgaac gcgccagcgg ccaagggtct
     6121 cgaccagcag acgccgattg agcgggtggt catcgacaac cagggcccgc acgccggcca
     6181 gaaggtcgcg gctcggctcg gcggcccggg cgctttcggg ttggcgctcg aacgatgccg
     6241 tgaaccagaa agtggatccg cgtccgacct ggctgcggac gccaatctct ccccccatca
     6301 actcgacgag ctgcttgcag atcgccagcc ccaggccggt cccgccatac ttacgcgtgg
     6361 tcgatccgtc ggcctggctg aaggctccga agatgcgcga ttgcgcttcg gggacaatgc
     6421 cgatgcccgt gtcgcggatc tcggctcgaa tcgtcgtgtg agtctccgtt gtctcgcgcg
     6481 actggatatc gagaacgatt tcgccgcggt cggtgaactt gatggcattg ccgatgaggt
     6541 tggtcaggac ctggcccaag cggcccggat cgccacgcag ccggcgaggc agcgcatccc
     6601 caaggcaata gaccagttcc acgcccttgg catgggcgga ctcggccagc aaatccaccg
     6661 tgccctcgac aacctcttgg agatcgaagt cgacatggtc gagcttcatc cggccggctt
     6721 ccatcctcga gaaatcgagg atgtcgttga tgatgttcag gagcaactca ccgctacggc
     6781 ggatggtctc ggcgccgtcc cgttgctcga ccgacagttc ggtttcaagc aggaggcctg
     6841 ccatgccgat gatggcatgc atcggggtgc ggatttcgtg gctcatgttg gccagaaact
     6901 cgcccttgag ccgcactgac tcgacggcag cgtcgcgggc cttctccaat tgtcgctcgg
     6961 cctcgacgag cgtcgtgatg tccttcgaca caccgatcgt gccgatgatg ttgccgttgc
     7021 gatcgcacaa cggcatcttg gttgtcagcg cccaggtctg gcgcccgtcg ggccacgtct
     7081 cgagctcctt cttggccacg atcggctcgc ccgtggcgat gatccgctgc tcgtcctcat
     7141 aggcctgacg ggcatgggct tcgccgaaga aatcgaagtc ggtcttcccc accacctggc
     7201 tcgaatcgcg cgcgccgaac atccgcgcca tttcgcggct gactcgcagg aaacgggatt
     7261 gccggtcctt gaaatagatg gcgtcgggga tgctgtccag gagcgcctgg agcaggtcgc
     7321 gctcgtgctc gatcgcctcc tccatccgct tgcgctcggt cacgtcccag aagatcccct
     7381 gcacgccggt caccttgccg ctggcgtcgt ggagcggcgt cttgatcacc tgaacatacg
     7441 tccgctcgcc attgggccgg acattctcct cgatcgcatc caggaccgtg agcgtgtcca
     7501 tcacacgctc atcgtcatcg cgatacttgg ccgccagttc cgacggatag aaatcggcgt
     7561 ccgtcttgcc gaggatttcc tcgggcggcc ggcccaccgt gcgacagaat cgttggttga
     7621 caaaggtgaa tcgcccttcg gtgtccttgc gaaagatgct ctgcggcagg caatcgacca
     7681 gcgaatgata gagcatctcc gagtcccaaa acgcctgctc ggtctcgcgg cgactgttgt
     7741 ccgcttcgga cgacgcgctc atgtgggcct cctcagactg agatcaacga cgccttgctt
     7801 ggatcgacca atccgatctc gtgcgccagc agggccgcct gctcgcgcgc cgccgccatt
     7861 ttcggatagc ggatttcccg gtagccggtg accgtctcgg ggaggcgcag cacgcggacg
     7921 caggcatcgt aaccgcgggg tgtgcgggat gcctcgtcga agcccgccaa gaggccgatg
     7981 taccagtcgc gaaagtcctt ctcctgccta tgccagtcgg gcatgacgcg ccgaagccag
     8041 cgctggtgct tgatcaacga gagcatccaa tcgcgcgtcc gcaggttgaa ggcaacctgg
     8101 cgaccgagca gggcgaattg cgggcgattg aggtgccgat agaccaggcg gtcgcctcgg
     8161 gcgggatcga cgttgtacca ggcccggtcg cggcggtgtt tctcttcgct ggtcaggagg
     8221 tgggccacgt acacttcgtc cttaatgagc atgaccttgt gaaggttcca gatcacggcc
     8281 ttggtggcct cgaagtcccg gcctgcgctg tcggacgcgc aaatgcgccg caccaacgcg
     8341 atgtaccgcg ccgcgtacgc gacgttctcg aactggatga gatcgtagac gcgcagagcg
     8401 aaatcggcca gcgcccggtc gtccatcggc agatcgcgga ccgcctcctc ggtcatcgca
     8461 cggtacgcgg cggccaaggc ctttccccgg cgccgcgtcc gcgcgagaat ctcggccttg
     8521 tcctccagaa ttcccgcata cgttgcggat tcgctgtgca ggccggcgct ttgccggaca
     8581 gcatcgaggt catgggccat cagtcgcccg agtgtgaatg ccttgacgtt ctcggacccg
     8641 gcggcgccaa gtccgctccg gatcgcccac tcgaacgtct cgaggcggag cgggatcagg
     8701 ccgcgttgga aggccaggcc gagcatgacg atgttggcgt acagttgcgt gccgaagtag
     8761 cactcggaga cctcggagac gttgcagctc agataggcct cggaccgtga gttgcgccgc
     8821 agcaagtctt cgagaacctt cacctggaag tcgtccttgc ccagcagact caggatggtc
     8881 ggcgtcttgt gcgtgttgac gacgaaggcc gtccgatgcc ggctgcccac gcgctggttg
     8941 gtcgacggat cgatggcccg agccgcttcg agaatgtcca cgccgatcac cagatccgct
     9001 ttgccgtagg ggatcaggtt cgaacaatag ggcgctccct cgaccgcata cgtgatcatc
     9061 gagtagaccc ccccgttccg aatcgccagc ccctttttgt cgcagaaccg gacctgatac
     9121 ccttcccgat tgcccgccag cacaaggatg gcggtggcgg ttccgatccc catcccgccc
     9181 accccggcca gatagccgtg ccagcgcccc tcgatcggcg gcggcgtggg cgtgggcagg
     9241 ctcgaggcat cgagccgctc gaagagtgtc ggcggcttct gcctgcgcaa gaccgtgacc
     9301 tcctcgaaag ccggacacgg ctggacccgc ggcccgtctt cgggcgtcga caccatggca
     9361 cgggcgcacg ccccatccgc cacacaccac gagaagtcga tctgcacttt ccgcccgtac
     9421 ggagtctgcg caaacgtcag cccggggcat ccggacgcct tcgtgcactc gaggcaattc
     9481 tcacagacct cgtgggtgat gttgatgaac cgtttctccg gcagaaagcc cttctcccga
     9541 agaatcctcc gctcctcgcg cgccgcgcgc cgatgatacg tgatgccgca ttccttgtcc
     9601 gccacgacaa ccttgacgcc gtcctgcagg atcaccttct cgagcagttc gcgatagctt
     9661 tcgcgctcgg ccggattcac ccgcacaacc gtcgcgccgg agcgcgtctg gtcggtgatg
     9721 ccggcaatca cggcgtcgat gttctgcgca aatgtccgct cccccatcag cccctggtcc
     9781 aaagccggcg tcgtctggtg tccggtcatc gccgtcgtct tgttgtccag cacgatgtag
     9841 gtgatgtcct ggccgttctt gagcgagttc gagatcgcaa tctgcccgct gtggaagaag
     9901 gtcgagtccc ccatgaacac aacctgtttg ttgcgaataa acgggtcgat gcccgccccg
     9961 gtcccgcctc ccagccccat gccgctgtag ttgtgcatca ggtccttggt cggctcgaac
    10021 atcagcatcg tgtagcagcc cgtgtcaccg tgaaaaacca ggtccaccgg cccgctcccg
    10081 tgcacccgcc gcatgtagcg cgaatcacga aactgcttct tcatctcgac caacacgctc
    10141 gccgagtccc gatgcgggca gcccggacag aatgtcgggg tccgcgccgc gatatcgacc
    10201 accaccgatt tgatttcgcg aatcagatcc aattcctgcc ggatgcgcgc tgcgtcaatc
    10261 cgcccttcaa gctcaggcag gctcagcaac agcggcccca gccggccgat caacttgctc
    10321 gggttcaaac cccgcgtcga cggaatcgga ttcagaccat gtgggaattg cttgccccag
    10381 acccggcaac acgaggccgg gccgctcccg gactgcttct gccaggcccg cgtgacgatg
    10441 gcaagcacct gctcctcgag aaagccgcga cgttcctcga tcacgaagac gtccttcagc
    10501 tcggatgtaa actcctggat cagcgccgga tcgagcgggt aagtgacccc gactttcagg
    10561 atcggaaaat gcccgtcgac tcccatctcc gcgagggcat ggcgcaggta acagtaggca
    10621 gccgccgaac tgatgaaccc gatccgagcc tcgaagttgg tatcatttcc tggcaaaatc
    10681 cgattggcgc cgtgccgacg cgcactggcc cagagccggg tatagcggtc ctccagaccc
    10741 tgctctttgc gccaggtgcg gggagggagc agaacattcc gctcgaaatc gatcttggcc
    10801 gtgtccaaat cgaacggagt gtgcaggttg agcgcaggag cgtggttggg ccggacgcgc
    10861 accgtgccgc cgccgtccgc ctggttggtc gtgaccaggt atccgatgta aagttcgctt
    10921 tcacgggaca gcgtaaacgc cagattgacc cagtccttga gttcctgggc gtcgctcggc
    10981 tcgagcaccg gcatcatcag gtgcttggcg atgtacctcg agtccgcggg tacctgggtc
    11041 gagtcgctcc acggatcgtc gcccagcaca atcacagccc ccccctccgg atgcgccccc
    11101 accagattcc ccaaggccaa agcgtcggca gcgacgtgca cgcccacgct cttctggaca
    11161 ctgattgccc gcaggccgtg catctgcgag ccgttgacca tagcggccga caacgcctcg
    11221 ttgttcgcca tcgtcgcccg aataccctgc tcggcaagca attcgtgaat ctcagaggcg
    11281 gtatcgaaga aaccggcgac gggtgagccg ggatagccag tccacagatg ggtgccgccg
    11341 acggtttcca ggcatccttt aacgagcagc tcgttccccg taaatacttc tataccaaaa
    11401 gttttggtaa accgcggatc gactttcacc aggcgcagta tgtcacccaa cccctgcact
    11461 ggcaagagtg gacttcctga tgcctatgcc ttactcagct tctgagccgc tgctgacaac
    11521 gaccgtgttg gtgaccggat ggttctgcca gacccagtca cccaccttcg ccctcccggc
    11581 gacgccgtga tgccgtgtca ccgcgacggc ggcctgatcg tcctggccat tcatccccaa
    11641 cgagtaaagt gtgaaacggt ccccttcccc gcgcctgtaa cgcaacggct gaccgttaac
    11701 ggggtccggc ggaacgggat tcagatacct gggtgcaagc gcttccagtg aatcggggag
    11761 agcgccgcgg tccagacggt gcctctcgat ggcgacggcg acgcgcgcca agttgaccgt
    11821 ggcctgggcc ttggcggcgt tccgggccgc tgcgctgacg ggcgggccca tcatcgaggc
    11881 aaagaggtta tagggggtgc gactccccaa tcgcgcctcg atcgactgct tgtccagccg
    11941 ggcgacatcg atcgtgccgg tgaccgtatc gaaggccggc agcaggtgct cctgatagag
    12001 acgcgcgatg tgatactgat tctggtactt catggggcca ggcaacgagt cgtagggtcg
    12061 ggtcgccgat gcgtcgatgc ccctgctccc cgcctcatga acctccgcgg cgccgccgat
    12121 cattccgatg gcgaaggcgc gctcgccacg cagacaatga gccatctctt tgacgaaatt
    12181 ggcgcgtctc aaggcaccct cgaattccgc aagctgaggc tcactccact catgccgcgc
    12241 caatccttcc cacaagcctt gcatcgccga atcggtcagc gcgatccgaa ccagggcggc
    12301 caccaggagc ggttcctccg acaatgcgtc cgccaaccgc agcgcggccc gcacatcccg
    12361 tgcggcagac gccgggtcgt ccgcggccag atgggcgagg gccgaacaaa cgagcggacg
    12421 agacatggcc ttgagcgagg ccaattgctg cagcaggagc tccatgtcct ccccgtaccg
    12481 ggccttgaga ttcgccgcgg gtcgctgggc ggcatcgcga atctcagcga gcgcggcctg
    12541 gttctgggcc aacgcaaaga gcaaatcctc ggccggtttc cccatcggcc tggcggccaa
    12601 ggcttcgaga acgggatgcg cagccttcga caccggctgc tgcgtcccat ccgaatccgc
    12661 cacaacatcg ggcggggcgt cggcaggcgc gcccgcggcg gtatcggccg cgccgtcgtt
    12721 cagtccgcgc aactgtcgct gccaaactgc caggtctctg aactggccca tttgccaccc
    12781 cggatttgtg tcgcgcaagc gcgaggtcca agcgaacatc tcagtcacac gatcgcaggc
    12841 cttcgaatcc cgccgtccga ctgctccgga agccccgtcc accacgtagt ctgagagcgg
    12901 acgcagcaga ggggtcatga agaagttgtt gtcatccggc accggctcgg gcagcaggcg
    12961 ccgaaaatcg agcgtctcac cctgcgcttc cagctcggcc cgatatcctt gccacaatcg
    13021 tcgggcgcga tagctctcga tggcgtaggc cagggcgagc aacgtgacga gggccaccgc
    13081 gatgaaaacc gcgcgcttaa gccatttggc gagtcgagac ggtttccagg cattcatagg
    13141 gtgaagaggt cggttgacga tgcattcgcg cctggttcgg ctcctccggc cgccgcggcg
    13201 gcgaggcctc tggaggcttc ggagcgggcg ccggatcgtc gagctgcagc gcattgaacc
    13261 cgcggaccgc agcctgatac gctaacgcca gacgcgcgtt ggcctgcgcc tcggccatct
    13321 cgaccgcgga cggcagcgcc gatcggttca ggatcacgac ggcaacccag cagacggcca
    13381 gggcagccca ggccgccggg gcgggccaga gccagtcgca gagccattct cccaggcgat
    13441 gaaacggcga gggtgcgcgg ctcgcagccg ccgcgccctc gagccgcgct tcccgcagca
    13501 actcggggcg ccactcgtca ggaatcggac ggaaagcctc cttccgcagc cgctgttcga
    13561 attcgtccgg gttcattgga tttctgcgta gaggggacgc agccgggcgc gcagtttgtc
    13621 tagaccgtag cggtaccggc tggctgccgt gttcaatgga atatcgagcg tcctgctgat
    13681 cgactcgaag gtcatctctt cccacagctt caggtgcaca acggccctct gctcaggcgg
    13741 cagttcggcg aacgcggcgt ccagttcacg acgaaatgtc tccgagtcgg gatcgtccgc
    13801 aggcgcgaac acaggttccg ccgcggtggc caaggtgttg cgaacacgtt cgcgggtcga
    13861 gtggcggcgg acctgatcca cccagaggtt atgcgccatc cgaagcagaa aggcgcgcgc
    13921 gtcgcgcacg ttcgcatcgc gaagcggtcg cactgccaat cgacggaaca tctcctggag
    13981 cagatcgcgc gtgtccgcct cgctgcgggt cagatcgagc gcaaaagcga acaacgcctg
    14041 ggcgtgatcg tcgtagattc gttccaaatc agaagccgca ggcatactgg tctgtcctgt
    14101 tgagaccaag acgcgcgccg cacggcggtt tgtctgaaga aaccgggcat ggcgcgtcgc
    14161 gccctccggg gcggaccgtg tcggctcacg ccttggcgcg tcgattgacc ttgtggatcg
    14221 ccgcctggtc ggtgcacttg atgatcatct cgtctatgca cacgtgcggg ggccggctcg
    14281 caacccagac catcgcttcc gccacatcct ccgacctcag agcctgaacg ccctcgtaga
    14341 ccttcttcgc ccgctcgatg tcgcccttga atcgcaccag cgaaaactcg gtctccgcca
    14401 tgcccggatc gatcgtcgca acgcgcaggc ctgtcccgca caattcgaga cgcaaggacc
    14461 gggtgatctg caactcgccc gctttggcgg cacaataggc ggctgcgccc tcgtaggcag
    14521 tgcgaccggc gattgatcct atattgatga tggttccggc ccgatgcggc agcatgagcg
    14581 gcaacaccgc ccgggtcatg cgcagcacgc cgagcacatt ggtctgcatc atcgtctccc
    14641 agtcggcgtc cttcccctcg actaccggtt cgagccccag cgccccgccg gcgttgttaa
    14701 cgagcacatc cacccgcggc gtgaactccc tcacccagct tgcgaacgcc tcgacactgg
    14761 ccgtgctcgc cacgtcgagc ggatggtagc cgacctcggc cgccccggcc gccttggctt
    14821 cgagcgccgc ggcgcttagc cgatcctccc ggcgggcgcc gagcagaagt cgagcccctg
    14881 cgcgcccgaa agccatcgcg ccggctgcgc caaaaccgct cgacgcgcct gtgatcaaaa
    14941 cccactgccc agtaaatttg taagacatgc ggttttttta ggacgcaacc gcccgcgcgc
    15001 caagccggaa gatcagtggg aagatcgatt gggaagacca gtgttcgccg ggataaacga
    15061 ccactccact ctcgctcggg cagagcggca attggtgcga ctggtgcgcg atagaggatt
    15121 tgaacctctg accccttccg tgtcaaggaa gtgctctacc cctgagctaa ccgcgcatta
    15181 actgccaaga tattatcggt atctcgccgt cacggcaaga acaaacccgt gggcctttcg
    15241 gccaaaaacc ttctcaaacc cgtcttcatt gctttgagga cctgcgcctt ggtcgctcca
    15301 gcatcgagtt tcagttccgt gtccagggcg tagagcttga aatagtaccg gtgggtacca
    15361 ctgggcgggc aaggaccgac gaagccgatg ggaccgaagt cattggtgcc ttacctggcc
    15421 ccattgagca acgtcttcgt ggtcattcga accgatagtg cttgcgcaca ggttccttga
    15481 cgtcccacac gcaggaaagc cctcgcggga aaaccaccag atcgcccttg ccaaacgaca
    15541 cgctctcccc gccgacttcg accgtcaccc gcccctccaa caggtaacag gtctcctggt
    15601 cgccgtaact ccagtcaaat cgtgacacct cgcacgacca gatcggccag gcaaaaacgt
    15661 ccagtgagcg caactcggcc tcagccggct ttcgtacctc gatcttcatg acctgatcct
    15721 aactcgccag caggggtaat tccagccttg gcctccgcat cttccccatg tccttgccag
    15781 gcagcgcgcg acaaaccaat cacgcgcgaa cgccaatatg ccctcttcca ccccccgatg
    15841 ccggttccgg gaccgcagct tgcttacgcc cgcatccctc tccgcccccg agggccgtgt
    15901 gcgcggcggt gggaacatgg cccaatacgg gcggtttgaa aatggaccgg g
//
LOCUS       DAOQRO010000022        15947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2752_length_15947_cov_4.057765, whole genome shotgun sequence.
ACCESSION   DAOQRO010000022 DAOQRO010000000
VERSION     DAOQRO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15947
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2752_length_15947_cov_4.057765"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..465)
                     /locus_tag="PKK20_01715"
     CDS             complement(253..465)
                     /locus_tag="PKK20_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98637.1"
                     /translation="MRINDHHSQARLEFVTLHEGTATLEMNVSEALQYTLERATGPTP
                     FTAWSPASAPVLVGEPVGETRVRFTD"
     gene            992..4261
                     /locus_tag="PKK20_01720"
     CDS             992..4261
                     /locus_tag="PKK20_01720"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNU98638.1"
                     /translation="MDSLPCNHWTRLAVTLGAVLAASVFGARSAPLATDLRCEYHRNP
                     VAIDVPQPRLSWVVQSGRDDRGVAQSAYQVLVASDAVLLARDEADLWDSGKVSSDRQA
                     HVVYEGKPLESGQACYWKVRIWDQEGRVSVDSQPAWWGMGMLKASDWKARWIGYEAPP
                     EAGDAASTAMKGLLRVDGQSWVWGSGARAGNQPAGKMHFRKVIQLPADRAVKEAFFLL
                     TADDGFQLFINGHPSGQGATWKTLVRVEVTGRLQPGANALGIQAANGGENPTPAGVIG
                     RLVVAFADGEPMIVPVDTSWQTASDAPERWSWPDFDAGGWKPASVVAEFGDQPWGKIA
                     LDEVRMEPAPFFRKAFQVRKPVRRATLYASALGVYELRLNGRAVDTDALSPGWTDYSK
                     RVRYFGYDVTGLVREGENVIGAMLGDGWYAGYLAFSGKRHYYGDKTRLIVQMQIEYAD
                     GSKELLGTDETWRATTGPIRENDLLMGSVYDARREMPGWDAPGYADATWQGVIVDAGV
                     RANLEAHPGTPIRRMEEVPARKMTEPKPGVYVFDLGQNMVGWVRLRAKGDAGQKVVVR
                     HAEMLNPDGTLYTVNLRAAKATDTYYLEDGDARFYEPPFTFHGFQYVEVTGLNYRPEA
                     GDVTGVVVYSDLPLAGTFECSEPLVNKLVLNSIWGQKGNFLDVPTDCPQRDERAGWTG
                     DAQVFMKTACFNLDAPGFYTKWLVDLCQDAQRADGAFGDVAPHVNIVGYGNTGWSDAG
                     PVCVWRMYEMYGDTRVLGEHYPALVRHMDYLARTSQDLVRGTGAYGDWLRLTGPQHSE
                     VIGTAYHYYAAHLMIGIADVLGKADDAARFRKLAEDIRGVFATRFIKPDGRILDAKNE
                     TGQTFYALAFGLNLVPTNLKAEVAAQFVASIRKEDDHLATGFLGTPFVLGALQKAGRP
                     ELAYKLVLNKTYPSWLQQVIWGSTTMWERWDGWRPDKGFQDPGMNSFNHYWLGCVSEW
                     LFTQAAGIDTDGPAFRRVVIRPEIVPGEGGLTWVRAIYHSISGEVRSAWKLSDGRFDL
                     EITVPGNCRATVYVPAQGVEAVTEGGRPAAEAHGVKYLRQEAGKAVFEVGSGCYAFQS
                     R"
     gene            complement(4272..5903)
                     /locus_tag="PKK20_01725"
     CDS             complement(4272..5903)
                     /locus_tag="PKK20_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HNU98639.1"
                     /translation="MKKGPSAAARRISLIANGDLRLSANQTCWAEQAKMEATLTAAIR
                     AEGWRVARAHPYDSVKKHGFIDSQKMGIEVFRGIDPDMPLVVAESVWQYSQHVLPGLL
                     THRAPILTVANWSGTFPGLVGLLNLNASLTKMGIRYSTIWSEKFTDPFFRNGLRQWLS
                     EGVVTHDQSHVRDLTLLKLPVADERVGRAFARRLRSDKAILGVFDEGCMGMHNAIIPD
                     ELLNPTGLFKERLSQSALYAAMQQVPDTEARGVLDWLLKKGMQFAWGRDEATELTEAQ
                     TLLQCKMYVAAVRLAAEYGCAAIGIQYQQGLKDLAPASDLVEGLLNNVSRPPVRCPRT
                     GRVLFEGEAVPHFNEVDECAGLDGLVTYRLWRELGFAPENTLHDVRWGAHYRGNGIND
                     YVWVFLISGAVPPAHFADGWKGARSERQPPMFFRLGGGTVKGVSKPGWVVWSRVFIMD
                     GKLHADLGIAESVALPARETERRWRLTNPEWPIMHAVLHGISRDQMMARHKSNHIQVV
                     YAPNIKQAHRACRIKAAALAELGLQVHLCGAVALA"
     gene            6355..7332
                     /locus_tag="PKK20_01730"
     CDS             6355..7332
                     /locus_tag="PKK20_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNU98640.1"
                     /translation="MNHSVLINRRTFIEHATILAAGSVVPLASGESASGGWQIGCYTR
                     PWDHYDYRVALDAIAEAGFKYAGIMTAKGRSWVMINPETTSEEAARIGWEVAQRGLKT
                     ASVYGDFQLGSSHAASVQALQRLIDHCVTCDSGDLLLGGTSEQQQYEPYYKAIADCCD
                     YAAERKVRMTIKPHGGQNATGRQCRQAIKKVGHRSFRLWYDPGNILYYSDGALDPVKE
                     AGFVDGLVVGMSVKDFRPPKEVMVTPGSGVVDFPKVLGRLRKGGFKQGPLVIECLAAG
                     DVATVTAEARRARLYLEQLVEQAALEATREARPALGTPAGRLRAGPRLF"
     gene            7345..8538
                     /locus_tag="PKK20_01735"
     CDS             7345..8538
                     /locus_tag="PKK20_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNU98641.1"
                     /translation="MTSTIAATPSPRLTSLDAYRGFVMFLMMAEVLRLCRVAQAIPDS
                     LFWGFLCHHQSHVAWIGCSLHDLIQPSFSFMVGVALPFSIASRMARGESRGRMYGHAL
                     WRAFVLILLGVFLRSTSSGRTNWTFEDTLTQIGLGYFALFVLGFRPVRDQWVGLGIVL
                     IGYWAAFALYPLPPADFDYARVGVSKDWPHLMSGFAAHWNKNSNLAWAFDAWFLNLFP
                     REHPFTHNGGGYATLSFIPTLGTMILGLIAGGVVRSARTSWQKVRWLAIAGAVGLGAG
                     WLLGAIGICPVVKRIWTPSWTVYSGGWCLLQLAAFYLVIDLWQRKAWAFPLVVIGMNS
                     IAAYCMAHLFDHFIAQNLKTHMGQDAFKLLGASYEPLLHGAATLLMLWLILYWMYRRR
                     LFLRI"
     gene            8615..9874
                     /locus_tag="PKK20_01740"
     CDS             8615..9874
                     /locus_tag="PKK20_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU98642.1"
                     /translation="MTSRERIRAAIHHEPTDRVPIDVGGTRQSGIAAVAYGRLKRHIE
                     IEPDSPVRVFDLFQMLAEIEPAVGDRLGNDCVGLHRRSVAFGIPNEDWKRFTFPDGFE
                     AEVPGGFNPRCGPDGELTIWREGVAAGKMPADGFYFDRLEKNPGALHPDLDAWEAPRL
                     TAHELAHAAREAARLESTTDKAIIAALGPPYELFHGLGQGGFDTWMITFASEPGYVRG
                     LYRKLTDAWIENLESFHGAVGDRIQVLQVCDDFGTQRAPFLSLRAFRELVMPAYRRGL
                     DWIHQRTPWKVMLHSDGALGPLLPSIVEMGVDILNPVQTSAVGMEPARLVHDYGDRLV
                     FWGGSCDPQGTLTSGSPVHVAAEARANLEHFTRCPGYVFASIHNIQANVPPENIVALF
                     ETAMRFHRARRAPDEGSFSRGRQRREH"
     gene            9915..11339
                     /locus_tag="PKK20_01745"
     CDS             9915..11339
                     /locus_tag="PKK20_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98643.1"
                     /translation="MKQTTPQITRRRFLGGAAVSTTAFLVAPGSVLGLRGQTPPSGKL
                     NIAGIGIGGQGASDLSSIQKESPGENIVALCDADWGHAAHTFKRYPGAKQYKDYRKML
                     DEMKEIDAVVIGTPDHHHAPASIEAIKRGKHVYCEKPLTHSVWEARKVAEAARQAGVA
                     TQMGNQGAGSEESRRLCELIWHGAIGKVREAHIWTDRPSQGLFNEYWPQGVPRPKETP
                     PVPETLDWDLWLGPAPERPYNPIYCPFRWRGWWDFGTGALGDIGCHAMDPVFRALKLG
                     APSSVEGSSTRVNEETFPLGSMVTYHYGERSAEAQVHNTYTAGLSGAAAGAVAMPPVK
                     LTWYDGGLRPPRPVGLEDEQVMGDNGRLLIGDEGFMLGNRIYPDARRREIGDIPRSIQ
                     RSVGHYKEWIEACKGGKKPASNFDFAGPLAEAVLLGNVALRVGLRERLVRYRLQWDAV
                     AFKFTNLDEANQYLRRAYRSGWSL"
     gene            complement(11630..12646)
                     /gene="hemC"
                     /locus_tag="PKK20_01750"
     CDS             complement(11630..12646)
                     /gene="hemC"
                     /locus_tag="PKK20_01750"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNU98644.1"
                     /translation="MTPDRPTSPDPPPPQRPIRLATRGSPLALAQTYLAIDRCRAAFP
                     DFAFEIRIIKTTGDRLQRASMAQTPALVSKGLFTKELETALLRNEADLAVHSLKDLPT
                     ELPSGLTLGAVLEREDARDVFVCRSAGSPLPTSTRAHASPLDALPPQATVATSSTRRR
                     EQLLELRPDLRVVQIRGNLSTRLDKLAHDPNLAATLLAAAGLRRLGYQLRPDGAIHGP
                     PQHPAPPGLRVALLPISLMIPCVGQAAIGIETRADDPLAQSLCLRLDHDVTRACVTAE
                     RAFLRAMGGGCLSPVAAYAEVDFGLLRIEGVSFRSGSAARAAATGPLNAPESLGQKLA
                     SRLS"
     gene            complement(12643..13668)
                     /gene="hemA"
                     /locus_tag="PKK20_01755"
     CDS             complement(12643..13668)
                     /gene="hemA"
                     /locus_tag="PKK20_01755"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNU98645.1"
                     /translation="MAVVVVGLSHNTAPVAIRERLSFPETAIPSALAQLRAQALAEES
                     VILSTCNRVEIYAAAPRAPQRTLAALRDFLLSYHGLDPNLAPELYGWHEPQSLEHLFR
                     VASGLDSMMLGETEILGQLKRAYALALRHHHTGRHLNKAFQRAFNVAKQIRTETSIQR
                     GSISVGSVVVDLAEKIFSSLAGRAVLVIGAGDISGKTARALLSRGASEVIVTNRSFDR
                     AAALAAELEGKAIPFDDWVGEFGRIDIAVSSTSAPHYILDRPRLEPLMRLRRQRPLLL
                     IDIAVPRDIDPDVNRMDNVFLYNIDDLQTITEAALRQRRQEIALCNELIRKRVQGLLD
                     PAPPPFP"
     gene            complement(13672..14493)
                     /gene="ccsA"
                     /locus_tag="PKK20_01760"
     CDS             complement(13672..14493)
                     /gene="ccsA"
                     /locus_tag="PKK20_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNU98646.1"
                     /translation="MFGVTDRNLFAVAVGVYGISTLYAILLWRKGFRTDNRVIYILLL
                     AAMGLQTSAMIKRGFSLSRCPVNNLFEATMFSMWAIVAAYLIIGLVRRLRFLGAFAAP
                     VLFCVGVFALMPGLDTRDPVPQFIPTMTSLHAALVMLSYGAFGLGAITAMMYLTQERD
                     LKRDKLRAVLSLFPPIQRLETVTWQSLVVGLALLSAGMVFGTIWLKHERGVFFLVDAK
                     ILWTIFIWLAYAILLLRRWQFQGGGRWFAWWAIGSFCFVLLTFAGFNQLSGIHNP"
     gene            14583..15131
                     /locus_tag="PKK20_01765"
     CDS             14583..15131
                     /locus_tag="PKK20_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98647.1"
                     /translation="MSTHCLLRREWIGAVGLATVAASPLSAFASQRQPARRFHVCLSP
                     VIVESDPDLLMSVRQAGVEVVWLAGYFYGHRPYADDLIRRARARLEKAGLESELITVP
                     LGLPGDSLGAHDGDFPLTPPRHWQTGQRPDGQSCAGTSLYAPATDENAAALRRLRAYG
                     FRRSFVDDDFRLGGLDVASMDA"
     gene            complement(15118..>15947)
                     /locus_tag="PKK20_01770"
     CDS             complement(15118..>15947)
                     /locus_tag="PKK20_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNU98648.1"
                     /translation="YRLFRPLFRPLFATLAGVGAQSDEDAARLVRLGCRPETVRVVGS
                     LKFDAAKLEERRLFDAAVLLRQIGVPDDAPILVAGSTHAGEEALLADILPRLRARHPD
                     LFLVLVPRHFERGREVGRELEARGIKFIYRREVTGSTQLAARSLECLLVNTTGELKDF
                     YETATVIFVGKSLTAEGGQNPVEPGALGKAMVFGPNMQNFAAIAESFVRSGGARQVRD
                     AAELEQTLDQLLADPAARAELGRNAVAVVKVNTGAIEKTVDMIVQSLAEEDNLVTRP"
BASE COUNT         2811 a         4580 c         5539 g         3017 t
ORIGIN      
        1 gacacggcag acaggccgct gcgctttgcg gcgcaggcag ggcacgcggc ctgcactggc
       61 aatctccatc cgttgtgggc ccgatgccct taccgggcga gagaacgccg acaacgacca
      121 gatgcactga tcaaggtccc gcggcgactg cggcgacggc ggtgacgctc cgattgtggc
      181 ggtgtgatct gctcagggag gggccgtaat gcggacgcgg taatagtgtt ggacagcctc
      241 ttccaccggg cgtcagtctg tgaatcgcac tctggtctcg ccgacgggct cgccaacaag
      301 caccggcgca gaggcaggcg accaggccgt gaacggcgtg ggaccggtcg cacgttccag
      361 agtgtattgc agcgcttcgc tgacgttcat ttcgagggtg gcggtgccct catggagggt
      421 gacgaattcg aggcgcgcct gggaatggtg gtcgttgatc cgcactgaga tttggcttgc
      481 ccggatcgga cctcgggcta tagcgcctgt cagcggaagc cgagattatc atgaagaccc
      541 cgcgttctat tgtgtcggcg gtgatccgac cgaggaacga cgcagatggc ccggtgccgc
      601 cccgctccga agggctgggg ggctttcacg gtgatttcat ttaaattcct gaacatcctt
      661 gtctcttcat gtcgctcgcc acagatcgat gaagtgtgcg atgaagtcca aaccgagaaa
      721 tgtccaaact tcagggccgc gtgcctccgt gcggcgcaag gatggacatc ggagcgggcg
      781 tcgggtcaag ccggcctgaa ggtggcacac cgaacggcgc gccgaaacgt cggtcgtgat
      841 cggaggttcc agcttcagcc ggttctcgcc gagggcccag gccaaaatgg gactgctgat
      901 ctcagccctc accagggacc gcgggtttgt ttccccttga aatggggctc ttggggtggg
      961 catgctgtga gcacgcaaag cacaatatcc tatggactct cttccctgca atcactggac
     1021 aagactcgct gtcacgctcg gggctgtttt ggccgcttcg gtcttcggag cccgttcggc
     1081 gccactggcc accgatctga ggtgcgagta tcaccggaac cccgtggcga tcgatgtccc
     1141 gcaaccgcga ctgagttggg tggtccagtc ggggagagac gaccgtggcg ttgcgcaatc
     1201 ggcctaccag gtcctggtcg caagcgatgc ggtcctgctt gccagagacg aagcggactt
     1261 gtgggacagc gggaaggtca gttccgatcg acaggctcac gttgtgtacg agggcaaacc
     1321 gctcgaatcc ggccaggcgt gttattggaa agtgcggatc tgggatcagg aagggcgggt
     1381 gtcggtcgac agccagccgg catggtgggg gatggggatg ttgaaggcgt cggattggaa
     1441 ggcgcgttgg atagggtacg aggcgccgcc ggaggcgggc gacgcggcga gcacagccat
     1501 gaaggggctg ctgcgggtgg atgggcaaag ctgggtatgg ggcagcggcg cgcgcgctgg
     1561 caaccaaccg gccggcaaga tgcatttccg caaggttatc cagcttcccg cggaccgagc
     1621 ggtgaaggag gccttctttc tgcttacggc tgacgatggt tttcaactgt tcatcaatgg
     1681 ccacccatcg gggcaggggg cgacgtggaa gacgcttgtt cgggtcgagg tcactgggcg
     1741 tcttcagccg ggggccaacg cgctgggaat ccaggccgcc aatggcggcg agaatcccac
     1801 acccgcgggt gtcatcgggc gcctggttgt ggcattcgcg gatggtgaac cgatgatcgt
     1861 gcctgtcgac acttcctggc agacggcttc tgacgctccc gagcgctgga gttggccgga
     1921 cttcgatgcc ggtggctgga aaccggcgag cgtggtggcg gagtttggcg atcaaccctg
     1981 gggcaagatc gcgctggacg aggttcgaat ggaaccggca ccgttctttc ggaaggcgtt
     2041 tcaagtgcgc aagccggtgc ggcgtgcgac gctttacgca tcggcattgg gcgtttacga
     2101 actgcggctc aacgggcggg cggtcgatac ggatgcgctg tcgccaggat ggacggacta
     2161 ttcgaagcgc gtgcgctatt tcgggtacga tgtgaccggg cttgtccgcg agggtgagaa
     2221 cgtcattggc gccatgctgg gcgatggctg gtatgcgggc tacctggcct tctcgggcaa
     2281 gcggcattat tacggcgaca agacgcgcct gattgtgcag atgcagatcg agtatgccga
     2341 cggttccaag gagttgttgg gaactgacga gacgtggaga gcgaccacgg gccccattcg
     2401 cgagaacgat ctgttgatgg gatccgtcta cgatgcgcgc cgcgagatgc ccggttggga
     2461 tgcgcccggc tacgccgacg cgacctggca gggggtgatt gtggatgccg gtgtgcgggc
     2521 caatctcgag gcgcatccgg gcacgccgat acggcgcatg gaggaagtac ccgcccgcaa
     2581 gatgaccgaa cccaagccgg gcgtgtacgt gttcgatctt ggccagaaca tggtgggttg
     2641 ggtgcgtctg cgggccaaag gggacgccgg ccaaaaagtg gtcgtccggc acgccgagat
     2701 gttgaatccg gacggcacgc tctacacggt gaacctgcgg gccgcgaagg cgaccgacac
     2761 ctattatctc gaagacgggg acgcgcgttt ctatgagccg ccgttcactt ttcacgggtt
     2821 tcaatacgtc gaggtgacgg gcttgaacta ccggcccgag gcgggcgatg tgacgggcgt
     2881 ggtggtgtat tcggatcttc cgctggctgg gacattcgag tgttcggaac cgctggtgaa
     2941 caagctcgtg ctcaactcga tttgggggca gaaggggaat ttcctcgatg tgcccaccga
     3001 ctgtccgcag cgcgatgagc gggccgggtg gacgggagac gcccaagtgt tcatgaagac
     3061 cgcttgtttc aacctggacg cgccgggttt ctacaccaag tggctcgtcg acctgtgcca
     3121 ggatgcgcag cgggccgatg gcgcctttgg cgatgtcgct ccgcacgtca acatcgtcgg
     3181 ctacggcaac accggctggt ccgatgccgg cccggtctgc gtctggcgca tgtacgagat
     3241 gtacggagac acgcgcgttc tgggcgagca ctacccggca ctggtgcgcc acatggatta
     3301 cctcgcccga acgagtcagg acctggtgcg cggcacgggc gcttacggcg attggctgcg
     3361 gttgaccggg ccccagcatt cggaggtgat tggcacggct tatcactatt acgccgcgca
     3421 tctgatgatc ggcattgccg atgtcctggg caaggcggac gatgcggcgc gtttccggaa
     3481 actcgcggag gacattcggg gcgtgttcgc gacgcggttc atcaaacccg acggccggat
     3541 tctcgatgcg aagaacgaga ccgggcagac tttctatgcc ttggcgttcg gtttgaatct
     3601 tgtgcccacg aatctgaagg ccgaggttgc cgcgcagttc gtggcgtcga ttcggaaaga
     3661 ggacgaccac ctggcgaccg gttttctggg gacaccgttt gtgctgggcg cgttgcagaa
     3721 ggccgggcgc ccggaactgg cctacaagtt ggtgctcaac aagacctacc cgtcatggct
     3781 gcagcaggtg atctggggct cgactacgat gtgggagcgg tgggatgggt ggcgtccgga
     3841 caagggcttt caggatccgg gcatgaactc gttcaaccac tactggctgg ggtgtgtgag
     3901 cgaatggctc ttcacccagg ccgccggcat cgataccgat ggtccggcgt ttcggagggt
     3961 tgttattcga cccgagatcg tgccagggga gggtggcctc acctgggtga gggccatcta
     4021 ccactcgatc agcggcgaag ttcggagcgc ctggaaactg agcgacggac ggtttgatct
     4081 cgagattact gtgcccggga actgtcgcgc cacggtgtac gttccggcgc aaggagtcga
     4141 ggccgtgacg gaaggcggac gacccgccgc cgaggcgcac ggtgtgaagt acctccgtca
     4201 ggaagcgggc aaggcagtct tcgaggttgg ctcgggttgc tacgcgtttc agagccgtta
     4261 gcgcgccttc ttcaggcgag ggccacagcg ccgcagaggt ggacctggag gcccaattcg
     4321 gccagggccg ccgctttgat ccggcaggcg cgatgggcct gcttgatgtt gggcgcgtac
     4381 acgacctgga tgtgattgga cttgtggcgc gccatcatct gatcgcggct gatgccgtgc
     4441 agcaccgcgt gcatgatcgg ccactccggg ttggtcaggc gccagcgtcg ttcggtttcc
     4501 cttgcgggca aggcgacgga ctcggcaatg ccgagatcgg cgtggagttt gccgtccatg
     4561 atgaacacgc ggctccagac cacccagccc ggtttcgaca cgcctttgac cgtcccgccg
     4621 ccgagacgga agaacatggg aggctggcgc tcgcttcggg cccccttcca tccgtcggcg
     4681 aagtgcgccg gtggaacagc tccggagatc aggaagaccc agacatagtc attgatgccg
     4741 ttgccgcggt agtgtgctcc ccagcggacg tcgtgcaggg tgttttccgg cgcaaagccc
     4801 agttcccgcc agagccgata ggtgaccagg ccatcgaggc cggcgcattc gtcgacttcg
     4861 ttgaagtgcg gcacggcttc gccctcgaag agcacgcggc ctgtgcgcgg gcagcgcacc
     4921 gggggacggc tgacgttgtt gagcaatccc tcgacgaggt cgctggcggg cgcgaggtcc
     4981 ttgaggccct gttggtattg aatgccgatg gcggcgcagc cgtattcggc cgcgagacgc
     5041 acggcggcca cgtacatctt gcattgaagc agagtctggg cctccgtcag ttcggtggct
     5101 tcatccctgc cccacgcgaa ctgcatgcct ttcttcagca gccaatcgag cacgccgcgc
     5161 gcctcggtgt ccgggacctg ttgcatggcg gcgtagagag cggactgact gagccgttcc
     5221 ttgaacaacc cggtcgggtt gagcagttcg tccgggatga tggcgttgtg catgcccatg
     5281 cagccttcgt cgaagacgcc aagaatcgcc ttgtctgacc tcaagcggcg ggcaaaggcg
     5341 cgtccgactc gctcgtcggc cacaggcagc ttgagcaagg tcaggtcgcg cacatggctc
     5401 tggtcgtggg tgacgacccc ttcgctcagc cactggcgca ggccgttccg aaagaaagga
     5461 tcggtgaatt tctcgctcca gatcgtgctg tagcggatgc ccattttagt cagcgaagcg
     5521 ttgagattga ggaggccgac caggccgggg aaagtgccgc tccaattggc gacggtgaga
     5581 attggagcgc ggtgcgtgag aagccccggg agcacatgct ggctgtactg ccagacgctt
     5641 tcggcgacga ccaacggcat gtcagggtcg atcccgcgaa agacctcgat ccccattttc
     5701 tgggagtcga tgaaaccgtg cttcttgacc gagtcgtacg ggtgggcgcg ggcgacccgc
     5761 catccctcgg cccggatggc ggcggtcaat gtggcttcca tcttggcctg ctcggcccag
     5821 caggtttggt tggctgagag gcggaggtcg ccgttggcaa tcagtgaaat gcgacgggct
     5881 gcggcagaag gtcctttttt catacaagaa tgatggcaag aggctaatcc ggaaggccgc
     5941 ccgactcaaa gcgaaaagtg agtgccgtgg gaggtcgctg gcatcctccg cctgcgcgac
     6001 cccgatttgc tggcatccgc ccgcctttgg aaatacgtga tcgagcatct gcatgccggc
     6061 attcgcgtcc tatgggagga ggcccgggag cgatgcaacg gcaagaggaa gcaagacgca
     6121 ctttgggatc atcgaattgg ttttcatgtt caactgtctt ctgctgaatc aaccgcccag
     6181 tgaacgttca ccgttttcac aaggccgatc cggcggttgc ctgagcggcc cttgattgac
     6241 cactctcatc aacagcaacc cttccggaac ttaaaataaa atgaccgtgt gcaggccgat
     6301 ggttgcttga aaacgcgatt gccttttagc gtgacttggg ttcacgctgc agcaatgaac
     6361 cactccgtgc tgatcaatcg acgaaccttc atcgagcacg ccacgatcct cgccgccggt
     6421 tccgttgtgc cactcgcgtc tggcgaatcc gcctcgggcg gctggcagat cggctgctac
     6481 acgcggcctt gggatcatta cgattaccgg gtcgcgctcg atgcgattgc cgaggcgggt
     6541 ttcaagtacg cgggcatcat gacggccaaa ggcaggtcgt gggtcatgat caacccggag
     6601 acaacgtcgg aggaggccgc tcgaattggc tgggaggtgg cacagcgcgg tctcaagacc
     6661 gcctcggtgt acggcgattt ccagctcggg tcctcgcacg cggcgagcgt ccaggcgctt
     6721 caacgcctga tcgatcattg cgtcacgtgc gattcgggcg acctcttgtt gggaggcacg
     6781 tccgagcaac agcagtacga gccttactac aaggcgattg ccgactgctg cgactatgcc
     6841 gccgagcgaa aagtgcggat gacgatcaag ccccatggag gtcagaacgc aaccgggcgc
     6901 cagtgccgcc aggccatcaa gaaggtgggg caccgcagct tccggctctg gtatgatccg
     6961 ggcaacatcc tctattactc ggatggcgct ctcgatccgg tgaaggaggc cggttttgtc
     7021 gacggcctgg ttgtcggcat gagcgtcaaa gattttcggc ctcccaagga ggtgatggtc
     7081 acgcccggga gcggcgtggt cgattttccg aaagtgctgg ggcgacttcg caagggcggg
     7141 ttcaagcagg gaccgcttgt gatcgagtgt ctggccgcgg gtgatgtggc caccgtgacc
     7201 gccgaggcga ggcgggcgcg tctctacctc gagcagttgg tggagcaggc ggcgctcgag
     7261 gctactcggg aggcgcgacc tgcattgggc acccctgccg ggcggctgcg cgcggggccg
     7321 cggctttttt gaagacgact gcttatgaca tccacgattg ccgcgacgcc atccccccgg
     7381 ctgacgtcgc tcgatgccta ccggggtttc gtgatgttcc tgatgatggc cgaggtgctg
     7441 cgcctctgcc gggtcgcgca ggcgattccg gacagcctgt tctggggttt cctctgccat
     7501 caccagtcgc acgtcgcatg gattggctgc tcgttgcacg atctgattca gccctccttc
     7561 tcgttcatgg tcggggtggc actgccattc tcgattgcca gccgaatggc gcggggcgaa
     7621 tcccgcgggc ggatgtatgg ccacgccctg tggcgcgcgt ttgtgctgat tctgctcggc
     7681 gtgtttctgc gatcgacgtc gagcggccgg accaactgga ccttcgaaga cacgctcaca
     7741 cagattggcc tgggttactt tgcgctcttt gtcctggggt tccgtccggt tcgcgaccag
     7801 tgggtggggc tggggatcgt gctgatcggc tattgggcgg cgtttgccct ctatccgctg
     7861 ccgcccgccg acttcgatta cgcgcgggtt ggtgtctcca aggattggcc gcacctgatg
     7921 agtggctttg ccgcccactg gaacaagaac agcaatctgg cctgggcgtt cgacgcctgg
     7981 ttcctcaacc tctttccgcg cgagcaccca ttcacccaca acggcggcgg ctacgccaca
     8041 ctcagcttca tcccgacttt gggcacgatg atcctcggac tgatcgccgg gggcgtggtg
     8101 cgcagcgcgc ggacctcatg gcagaaggtg cggtggctgg cgattgcggg cgcggttggg
     8161 ctgggggcgg gctggctgct tggggcgatc gggatttgtc ccgtggtgaa acgcatctgg
     8221 acgcccagtt ggacggtcta cagtggcggt tggtgtttgc tgcagctggc ggctttctac
     8281 ctggtgattg atctgtggca gcgcaaggcc tgggcgttcc cgcttgtggt gatcggcatg
     8341 aactccatcg ccgcctattg catggcccat ctgttcgacc atttcattgc gcagaacctc
     8401 aagacccata tgggtcagga cgccttcaaa cttctcggcg cgtcctacga acctttgctg
     8461 cacggcgcgg ccaccctgct gatgttgtgg ttgatcctct actggatgta ccgccggagg
     8521 ctgttcctga ggatttgacc cgcatgtttt acacccggtc ggcgcacatg gcgatcggac
     8581 ttcgcaagag gactgtcatg cgggtttgaa aagcatgacc tcgcgcgaac gcatccgagc
     8641 tgccattcat cacgaaccga ccgatcgtgt cccgattgat gtgggcggga cgcgccagag
     8701 cgggattgcc gcagtcgcat atggccggct gaaacgccat atcgagatcg agccggacag
     8761 tccggtccgc gtcttcgact tgttccagat gctggccgag atcgaaccgg cggtgggaga
     8821 ccggctcggc aacgactgcg tgggattgca tcggcgctcg gtggcgttcg gcatccccaa
     8881 tgaagactgg aagcgattca cgtttccgga cggattcgag gctgaagtcc ccggcgggtt
     8941 caatccgcga tgcgggcccg atggggagct gacgatttgg agagagggcg ttgccgcagg
     9001 gaagatgccg gccgatggat tctacttcga tcggctcgaa aagaaccccg gcgcgttgca
     9061 tccggatctc gatgcctggg aggcgccgcg tctgacggct cacgagttgg cgcacgcggc
     9121 gcgcgaggct gcgcgcctcg agtcaacgac cgacaaggcc atcatcgcgg ccctgggacc
     9181 gccgtacgaa ttgttccatg gactcgggca gggcggattc gacacatgga tgatcacgtt
     9241 tgccagcgag cccggctacg ttcgggggct gtaccgcaaa ctgacggacg catggatcga
     9301 gaatctcgag tcgttccacg gcgccgtcgg cgaccggatt caggtccttc aggtgtgcga
     9361 cgatttcggg acgcagcggg ctcccttcct ctctctgcgc gcgtttcgcg agctggtgat
     9421 gccggcctat cgtcgagggc tggactggat tcatcagcgc actccctgga aggtcatgtt
     9481 gcactccgac ggcgccttgg gtccgctgtt gccttccata gtggagatgg gcgtcgatat
     9541 tctgaatccg gttcagacca gcgcggtggg gatggaaccg gcccggttgg tgcatgacta
     9601 tggagatcgg ctggtgtttt ggggcggaag ctgtgatccg cagggcaccc tgacgtccgg
     9661 ttcgcctgtg cacgttgctg cggaagcgcg cgcgaacctc gagcatttta caaggtgccc
     9721 cggttacgtt tttgcttcga ttcacaatat ccaggccaat gtcccgccgg agaacatcgt
     9781 ggcgctgttc gagacggcga tgcgctttca tcgggctcga cgcgctccgg atgaaggttc
     9841 attctcgcgc ggacgtcaaa ggcgagagca ttgatgccga gcgtccctgg ggcgctgtcg
     9901 ccagacaatc gaatatgaaa caaacgactc ctcaaatcac acgtcgccgt tttttgggcg
     9961 gcgccgccgt gtccacaaca gcgttcctcg tcgcgcccgg ttcagttctg ggtctgcggg
    10021 ggcagactcc acccagcggc aaactgaaca ttgccggcat cgggataggg gggcagggcg
    10081 cctccgacct gagcagcatt cagaaagaga gtccgggcga gaacatcgtg gcgttatgcg
    10141 atgccgactg gggccatgcg gcgcacacat tcaaacggta tcccggggcc aagcagtaca
    10201 aggattaccg gaagatgctc gacgagatga aggagatcga cgctgtggtg atcggcacgc
    10261 ccgaccacca tcacgcgcct gcttcgatcg aggccatcaa gcggggcaag catgtctact
    10321 gcgaaaagcc gctgacgcat tcggtttggg aagcgcgcaa ggtggccgaa gcggcgcggc
    10381 aggcgggagt cgcaacccag atggggaatc agggcgccgg ttccgaggaa tcccggcgct
    10441 tgtgcgaact gatctggcac ggagccatcg gcaaggtccg cgaggcgcac atctggaccg
    10501 accgtccttc gcaaggcctg ttcaacgaat actggcccca gggcgtgccc cggcccaaag
    10561 agacgccgcc tgtgcctgag acgctcgatt gggacctgtg gctcggtccg gcgcccgaac
    10621 gcccctacaa cccgatctac tgtccgttcc gctggcgcgg ctggtgggac ttcggcacgg
    10681 gcgccctggg agacattggc tgtcacgcga tggaccctgt cttccgcgcg ttgaagctgg
    10741 gcgcgccttc gagtgtcgag ggatcgtcga ctcgcgtgaa tgaggagacc ttcccgctcg
    10801 ggtcgatggt gacctatcat tatggagagc ggagcgcgga ggctcaggtt cacaacacgt
    10861 acacggcggg tctgagcggt gcggcggcgg gtgcggtggc gatgccgccg gtgaagctga
    10921 cctggtacga cggcggcctg cgtcccccgc gaccggtcgg gctcgaagac gagcaggtca
    10981 tgggcgacaa cggacggctt ctgattggcg acgagggctt catgctgggc aatcgaatct
    11041 accccgatgc gcggcgcaga gagatcggtg acatcccgcg ttcgatccag cgttcggtcg
    11101 gccactacaa ggagtggatc gaggcttgca agggcggcaa gaaacccgct tcgaacttcg
    11161 acttcgcggg gccgcttgcc gaggccgtgc tgttgggcaa tgtggccttg cgcgtcgggt
    11221 tgcgtgagag gcttgtccgg tatcgcctcc aatgggatgc ggtcgccttc aagttcacga
    11281 atctcgacga ggccaaccag tacctcagac gcgcgtatcg gtccggttgg tccctctgac
    11341 gcgccataga gccgggcgcg actgccccca gagcatcgcg cctggtgaaa cgccggagcg
    11401 ctcggagagc cggctcgagg cgtgcggaga tcggaggacg gtgcgtcggt agccggctca
    11461 aagcctctgt cgtcaggtag ggtgacggtc gactttcacg tccccgctcg acgggtaagg
    11521 cgagcgttga cattccacgg ttgaatgcgc acgcggggac caggaaccag gaacgttcaa
    11581 ttccaaacgg tcaattctca atcagccgga agcgcaaagg aacctgttgt cacgacaacc
    11641 tggaagcgag cttttggccg agggattccg gagcgttcag cggtccggtg gccgcggcgc
    11701 gcgcggcgga gccgctccgg aaggagacgc cctcgattct gaggagaccg aaatcgacct
    11761 cggcgtaggc cgccaccgga ctgaggcaac cgccgcccat ggcgcgaaga aacgcgcgtt
    11821 cggccgtcac gcaggcacgt gtgacatcat ggtcgagccg caaacacagc gattgcgcga
    11881 ggggatcgtc agctcgggtt tcaatgccga tcgcagcctg gccgacgcaa gggatcatga
    11941 ggctgattgg cagaagagcc acgcgcagac ctggcggggc ggggtgttgc ggcgggccat
    12001 gaatcgcgcc atccggtcgc aactgatagc caaggcgacg cagtccggcg gcggccagca
    12061 gcgtggcggc gagattcgga tcatgggcca gtttatcgag ccgggtgctg aggttcccgc
    12121 ggatttggac cacgcgcaga tcggggcgca attcgagcag ttgttcgcgt cggcgggtgc
    12181 tgcttgtggc gacagttgcc tgaggcggca gggcatcgag tggggacgcg tgcgcgcgtg
    12241 tcgaggtggg gagtggggaa ccggccgatc ggcagacgaa gacgtctcgg gcgtcttcgc
    12301 gttcgagcac ggcgcccagg gtcagtccgg agggcagttc agtgggcaga tccttgaggc
    12361 tatgaacggc caggtccgcc tcgttgcgga ggagcgcggt ttccagttcc ttggtgaaaa
    12421 ggcctttcga gaccaaagca ggcgtctggg ccatcgaggc gcgctggagt ctgtcgcctg
    12481 tggtcttgat gatgcggatc tcgaaggcga agtccgggaa ggctgctcgg cagcggtcga
    12541 ttgcgaggta tgtctgggcc agtgcgagcg ggctgccgcg ggtggccagg cggatcgggc
    12601 gctgtggggg gggcgggtca ggagaggtgg ggcggtcggg ggtcatggga aaggtggagg
    12661 ggcggggtcg aggaggccct ggacgcgttt gcgaatgagt tcattgcaga gggcaatctc
    12721 ctgccgacgc tggcgcaggg cggcctcagt gatcgtctgt aagtcgtcga tgttgtagag
    12781 aaagacgttg tccatccgat tcacatccgg atcgatatcg cgggggacgg cgatgtcgat
    12841 caggagcagc gggcgttgtc ggcgcaggcg catcaggggt tcgaggcgcg ggcgatcgag
    12901 gatgtagtgg ggggcagagg tgctgctgac ggcgatgtcg attcgtccga attcgccgac
    12961 ccagtcgtcg aacggaatgg ccttgccttc gagttccgcg gcgagggcgg cggcgcggtc
    13021 gaacgaacgg ttggtgacga tcacctcgga agcgccgcgg gagagaaggg cgcgcgcggt
    13081 cttgccgctg atatcgccgg ccccgatgac caggacggcg cgtccggcca gcgaactgaa
    13141 aatcttctcg gccagatcga cgacgaccga tccgacggag atgctgccgc gctggatgct
    13201 ggtctcggtc cggatttgtt tggccacatt gaaggcccgc tggaaggcct tgttcaagtg
    13261 ccgtccggta tggtgatggc gcaaggcaag cgcgtaggca cgcttgagtt gtccgaggat
    13321 ttccgtttcg cccagcatca tggaatcgag tcccgacgcc actcggaaca ggtgttcgag
    13381 gctttggggc tcgtgccaac cgtacaactc cggggccaga ttcgggtcca atccatgata
    13441 gctcagcagg aagtcgcgca gtgcggcgag tgtacgctgg ggggcgcgcg gagcggcggc
    13501 atagatttcg acgcggttgc aggtggagag gatgaccgat tcctcggcga gcgcttgggc
    13561 gcggagttgg gcgagggcgg aagggatggc ggtctcgggg aaggagaggc gctcccggat
    13621 tgccacgggc gccgtattgt gactgagtcc gacgacgaca actgccatgg ctcacgggtt
    13681 gtggatgccg gacaactggt tgaagcctgc gaacgtcagg aggacgaagc agaaactgcc
    13741 aatggcccac caggcgaacc agcgcccgcc gccttggaac tgccaccggc gcagcagcag
    13801 gatggcgtag gccagccaga tgaaaatcgt ccagagaatc ttcgcgtcga ccaggaagaa
    13861 cacgccgcgc tcgtgtttga gccagatggt cccgaacacc attccggccg agagcagcgc
    13921 gagtccgact acgagcgact gccaggtgac cgtttcaagc ctttggatgg gaggaaacag
    13981 cgagagcacc gccctgagct tgtcgcgttt caggtcgcgt tcctgggtca ggtacatcat
    14041 tgccgtgatt gcgcccagtc cgaacgcacc gtacgagagc atcaccaacg cggcgtgtag
    14101 gctcgtcata gtggggatga actgcggtac cggatcgcgt gtgtccagtc cgggcatgag
    14161 agcgaagacg ccgacgcaaa acagcacggg cgccgcgaat gcgccgagaa aacggaggcg
    14221 ccggacgagt ccgatgatca ggtacgcggc cacgatggcc cacatcgaga acatggtggc
    14281 ttcgaagagg ttgttgaccg ggcatcggga cagcgagaaa ccgcgtttga tcatggcgct
    14341 ggtctggagc cccatcgcgg ccaggagcag gatgtaaatg acgcgattgt ctgttcgaaa
    14401 ccctttgcgc caaagcagaa tcgcgtaaag ggtgctgatg ccatacaccc ccaccgcgac
    14461 cgcgaacagg tttcgatccg taacgccgaa cacaggctga gggtaaaccg gtgcggagcg
    14521 cctgcaagct tggagaggtc cgggcgcgtg acttgagtgt tcatcggcga ttgaattggg
    14581 gcgtgagcac acattgtctc ttgcggcgcg aatggatcgg ggcagtcggc ttggcaactg
    14641 tcgcagcgtc tccgctgtcg gcgtttgcca gccagcgcca gccggcgcgg cgctttcacg
    14701 tctgcctctc gccggtgatt gtcgagagcg acccggattt gctgatgagc gtgcgccagg
    14761 ccggagtgga ggttgtgtgg ctggccgggt acttctacgg ccaccggcca tacgccgacg
    14821 atttgatccg ccgcgcccgc gcgcggctcg agaaggccgg cctggagtcc gaactgatca
    14881 cggttcccct cggtcttccg ggcgattccc ttggcgcgca tgacggcgac tttccgctga
    14941 cgccgccacg gcattggcag accgggcaac gccccgacgg gcagagttgc gccggcacgt
    15001 cactgtacgc tcccgcaacg gacgagaacg cggctgcgct gcggcgtctg agggcttacg
    15061 ggtttcgccg gtcatttgtg gatgacgatt tccggctggg aggactcgat gtggcgtcta
    15121 tggacgcgtg accaggttgt cctcctcggc gagggattgg acgatcatgt cgacggtttt
    15181 ctcgatcgcg cctgtgttga ctttcaccac ggcgacggcg ttgcgtccca actccgcgcg
    15241 ggcggcgggg tccgccagca attgatcgag cgtttgttcg agttcggcag cgtctcgaac
    15301 ctgcctggcg cctccgctcc tgacgaagct ctcggcgatc gccgcgaagt tctgcatgtt
    15361 gggcccgaac accatggcct tgccgagtgc gcccggctcg acgggattct gcccgccttc
    15421 ggccgtgagg cttttgccga caaagatgac ggtggccgtt tcgtagaagt ccttgagctc
    15481 gccggttgtg ttcaccagaa ggcattcgag gctgcgcgcg gccagttggg tcgagccggt
    15541 tacctcgcgt cggtagatga acttgatgcc gcgggcttcg agttcgcgtc cgacttcccg
    15601 cccccgctcg aagtggcgcg gcacaaggac aaggaacagg tcgggatggc gtgcgcggag
    15661 gcgcgggagg atgtcggcga ggagggcttc ctcaccggcg tgcgtgctgc cggcgacgag
    15721 aattggtgca tcgtcgggaa cgccaatctg acgcaggagc acggcggcat cgaacagacg
    15781 ccgttcctcg agtttggcgg cgtcgaattt caggctgccg accacgcgca cggtttcggg
    15841 gcggcatccc agtcgaacca ggcgcgccgc gtcctcatcg ctttgtgcgc cgacgccggc
    15901 gagcgtcgcg aagagcggtc ggaacagcgg tcggaacagg cggtacc
//
LOCUS       DAOQRO010000023        15886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2780_length_15886_cov_5.894700, whole genome shotgun sequence.
ACCESSION   DAOQRO010000023 DAOQRO010000000
VERSION     DAOQRO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_15886_cov_5.894700"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..476
                     /locus_tag="PKK20_01775"
     CDS             132..476
                     /locus_tag="PKK20_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNU98649.1"
                     /translation="MMRSVNLEVARPSVDEARRRLLGAIDAARRSRVRVLKVIHGWGS
                     SGEGGALGPAIRRSLRLRVKEGRAVAVVPGERFSSDTAEGRELLRRYPSLHSDRDFNR
                     ANPGITIVELAP"
     gene            complement(761..1732)
                     /gene="galE"
                     /locus_tag="PKK20_01780"
     CDS             complement(761..1732)
                     /gene="galE"
                     /locus_tag="PKK20_01780"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNU98650.1"
                     /translation="MNVVVTGGAGYIGSICVEELLNAGHAVTVFDNLTEGHRAAVDSR
                     ARFIFGCLSDRAAILRTLRETHAEGVLHFAANALVGESMTNPTKYFRNNVAYGLNLLD
                     AAVESRVRKFVFSSTCATYGPPERVPMTENLPQRPINPYGESKLMFEKMLEWFRQIHG
                     LEFVAFRYFNAAGASARFGEHHRIETHLIPNVLRVPLGQSSHCEIFGTDYPTPDGTCI
                     RDYIHIVDLADAHIRALEPGKNGFYNLGNGEGYSVRQVIQACEKVVGQSIPALEKPRR
                     AGDPPRLVASAAKAALELGWKPRYPKLEDIVATAWAWHKAHPNGYPD"
     gene            2007..3329
                     /locus_tag="PKK20_01785"
     CDS             2007..3329
                     /locus_tag="PKK20_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98651.1"
                     /translation="MNTFQIHISRRRFLARSAVAAGLPLWCLDELHSQAAQTRPLAAN
                     DKPGIGLIGCGGQGRGDCNWARNYGTVIAVADVDDGHAEEAQKQFGAAHRFKDFRKLL
                     ERDDIHVIINGTPDHWHTLINIASARAGKDIYSEKPLTLTIAEGKTLVQEIRKHQRVL
                     QVGSQQRSDARFRLACELVRNGRIGRLEHMVVGLPTGPREGPFSAVPVPPELDWDYYL
                     GQTPKVDYNGHNSHWNFRWWYQFSGGQITDWGAHHNDIAQWGNGTERSGPVEVDGKGL
                     VEMIPGGYDAMAKYRIDCKYANGVTMTIVDEGTVTDRNVVGGGKSTPNGVQFIGTDGW
                     IFVSRSEIKANKQELIEEPLPSGATRLHKSDNHMGNFFECVRSRKDPICDVEIGHRSI
                     SIAHLGVISVRMKRPLRWDPSREQFIDDAEADRWLSRAMRAPYDMSFI"
     gene            3635..5233
                     /locus_tag="PKK20_01790"
     CDS             3635..5233
                     /locus_tag="PKK20_01790"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNU98652.1"
                     /translation="MSAAVPCVLFEDDHLLVVNKPPGWNTHAPSPYAGEGIYDWLRHR
                     SPRWANLAIIHRLDKETSGVLVFSKSALANRELTRQFTARQVKKQYLLVADRPVPPAP
                     LRFASHLVRDGDRYRSQSTGGSGTMAETVFQRAERMGDRTTVEAFPTTGRTHQIRVHA
                     AAAGFPILGDPLYGGTPARRLHLHAASVTLRHPATKQVVVFRAPADWDREPAQALRGS
                     FIDPKQTDAYRIIHGAADGWPGWYVDRLGRFLLSQGEGQLTGGQKAALERWMAGVSAR
                     GVYHKQRDRGVCSSAPEDVAPRRVFGEAAYGDVMVRENGLRYAVRFDQGYSIGLFLDQ
                     RDNRRRLLTGHIAGGLSAYPDGCKGIEVLNLFAYTCAFSVAAARAGARVTSVDLARKY
                     LDWGRRHFEANQLEANAHDFLYGDAFDWLRLLAKRARRFNLVIVDPPTFSTSKTGGRF
                     RAEADWPRLARAALAVLAPGGWLFASTNAARVEPGRFLDQLVAAAGEARRRVLDRHYA
                     PQPPDFPICRAEPAHLKTVWLRIE"
     gene            complement(5246..7702)
                     /locus_tag="PKK20_01795"
     CDS             complement(5246..7702)
                     /locus_tag="PKK20_01795"
                     /inference="COORDINATES: protein motif:HMM:NF027717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5010 domain-containing protein"
                     /protein_id="HNU98653.1"
                     /translation="MNTVCLSILGLGLALCAGLTSSGAMAAEINPPIGPHVNLEPDDF
                     AQAVSFTAQDRVVLTSYFYWYDVYSGAHIRNGDGSDALTDHPPTLTGFSYKSTSWHRQ
                     QLLDMMAAGVDVLLPVYWGEPSQRVAGKPVLEQPWSFAGLPPLVAALDALRAEGKQPP
                     RVGLFYDTSTLEYNAAGRRIDLTTDYGRQWFYESIRDFFSLIPPRHWAMIDDRPVVFL
                     YAAGFAAAHDQTCIDFVAESFPRDFGGRRPYIVRETSWNVRADNTYAWGGAISLKNLS
                     VAALGPGYDHSAVPGREPLVVPREDGAFFERNWTRFLRNPSLLVHVETWNEYHEGTDI
                     AASKEYGRQYIDLNRKFADMFKAGIKPPRPRGPYSEVRSVTTSLGATNESKGLIQFDH
                     ADGVTRPATAGGHECRESVSTVHQGKYIYFQVHDSFKWADSMLVDVDVEYFDAGTGSF
                     RIEYDGPDPNAPFNGAYTASKTSVTLNNTRQWKKARFRLLESRFLNSQNGGADFRIAI
                     QAPALTVRRVTVTRLGLPAEAGQTLRGWQEDFSGALGPEWSITGDDADFFRVADGLLR
                     LSPAPGATRRLLLEQPRPGLESCEILARIRTVGPTPDDGPWGGLALGVDPAARTGLAC
                     QLHSNAASGRGIGLSGDSMPQGPRAGFAWQPNTWYWLRIRHEVRDLTGAPELQARLWR
                     ADGDTPEPEAWLLWWDYYPASAARHGYSGWIAGSVGSGLESDYFLLKSDALGEITVRL
                     PALKPSRPSLELREWSPQAGTTLDLHGTADTTYAIESSIDLADWDAQIAVTDTAGRST
                     FRDVAATNRPRAFYRARTTK"
     gene            complement(7831..8709)
                     /locus_tag="PKK20_01800"
     CDS             complement(7831..8709)
                     /locus_tag="PKK20_01800"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNU98654.1"
                     /translation="MSQLIDAAIGHLEDLRARGVRFVPVHANTWAALNQSCPRPPAPS
                     RRAPLSSAATPRSVPTPAPSAAADQAGLKPDRPDSSMRSPDDKETALEALRARVLPCR
                     KCEHLARSRRTVVFGTGSARAELMFVGEAPGADEDAQGIPFVGKAGQLLTRIIQTMGL
                     SRETVYIANILKCRPDTPGQSSGNRKPTPIEMATCLPHLLAQIDIIQPKVLVALGGTA
                     VEGLMGKMEGITKLRGHWLEFHGIPLMPTYHPAYLLRNQALAEKRKVWEDMLEVMARL
                     GLPISRKQRNFFLRGT"
     gene            complement(8730..10064)
                     /locus_tag="PKK20_01805"
     CDS             complement(8730..10064)
                     /locus_tag="PKK20_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98655.1"
                     /translation="MTSTFRSSRRAFLRQVGAAGLAAPFVARYSWSASPNEVLRHASF
                     GASGMAAADLESITRHPKVRLVAVADVDLPRADGLKAKFPELKVYQDWRKLLDEVKGL
                     DSVNVSTPDHMHAPIGMSAMQLGKHMYGEKPLTHDVYEARQLTLFARQQRLATQMGIQ
                     IHSASEYRLAVRLIQDLAIGKVREVHTWSGKKWGDMTPRPDRTDPVPAGFDWDLWLGV
                     AASRPYIGGGYYHPGNWRKRLDFGTGTFGDMGCHIYDPVFKALALTAPLSVRSEGPPP
                     NAWNWSVDAVIHYVFPSTPFSEGSTVKVTWYDGDQRPPKDVQALIGQQPVPDQGSIFI
                     GTKGVLLLPHIGPPQLLPEKQFEDYPVPRVSGANHWHEWVEACLGNGTPSANFDYSGP
                     LTEAVLLGGVATHFPQTTLEWDAANVQFKNVAEANKFVRRQYRAGWEVKGLS"
     gene            complement(10303..10860)
                     /locus_tag="PKK20_01810"
     CDS             complement(10303..10860)
                     /locus_tag="PKK20_01810"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNU98656.1"
                     /translation="MRVVVAITGASGAVYAQRLLDCLDVRQHQVHLLMSRYAAEVVAE
                     ELPGGLRLAEGVVSHALDDMNAPFASGSNAPDAMVVIPCSMGTLGRIAHGLSGDLLLR
                     TADVVLKEHRKLILVPRETPLNLVQIKNLELLALAGAILLPANPSFYSRPRTAEDVAD
                     TVVARVLDHLGIAHQLVARWREEAD"
     gene            complement(10863..11471)
                     /locus_tag="PKK20_01815"
     CDS             complement(10863..11471)
                     /locus_tag="PKK20_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98657.1"
                     /translation="MAHEPENPLDALWTEHSRVFHDFDDLTLARWMAQTLGQLQGRGW
                     RLSHPLLAAYRLAAHVGHDRQIWLKRLAMSPANYPDAPCCRAPLVPMLSRDVLESGLV
                     CLHCNSTAVPFDDLPEDLRPLIRGWAEEYAPVHAVAHWDDGERKRVGDYDAALDDAAA
                     KAETLLAFAGHQLVPKLLEVYPAVAWEDQDECLDVRAEDVKL"
     gene            complement(11596..12357)
                     /locus_tag="PKK20_01820"
     CDS             complement(11596..12357)
                     /locus_tag="PKK20_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNU98658.1"
                     /translation="MNTSIEVLFTPAEFAALAQRDLSETACVVFDILRATSSMVTALA
                     HGAQAVVPVATIPEALAWKERDPDVLLAGERDGLRIGAALTGGVEFDLGNSPREFVPE
                     RVRGRTIVMTTTNGTRALQACRPAAHVWVGAFLNLSAVVEALRHVQPRHLLVVCGGTI
                     ERAAFEDALAAGALCEAVWELYREGEVLDSVLLALEAWCRHANDVAAGLGSGRNGRRL
                     LARPELRDDVSFCAMRDRYAFAPTLDADAIVRRLK"
     gene            complement(12643..13551)
                     /locus_tag="PKK20_01825"
     CDS             complement(12643..13551)
                     /locus_tag="PKK20_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNU98659.1"
                     /translation="MSTNFPNRPSSPRLTRVEHTTFRVRPYDLAATLNSGQAFRWRPA
                     GAGFEGVVGRRWTRLEPCAEGIRAETPAPVPDWDWLATYLQVGERLDTVLASFPNDPI
                     LADAVRHFPGLRLLRQDPWECLASFILSATKQIVQIRQMIGLLCARYGEPVPVPRGHA
                     PEFAFPTAERLAQCTEAELRACKLGFRAPSLRAAAQSVAAGRPNLATLPSLELEEARE
                     VLMALRGVGRKIADCVLLFAYGFPQAFPIDVWIERVLRRLYFPRRRVTPSGLRRFAGT
                     HFGPRAGYAQQYLFHYIRTVDRQRSR"
     gene            complement(13560..14099)
                     /locus_tag="PKK20_01830"
     CDS             complement(13560..14099)
                     /locus_tag="PKK20_01830"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNU98660.1"
                     /translation="MNSPQDPSPAELEQAIRNVPDFPKPGIQFKDITPLLGNARLFAA
                     TVDRLIAGHPPGSIHAVAGIDARGFLFASAAALKLGAGCVPIRKRGKLPYRTLEESYE
                     LEYDSNTIAIHADAVPEGSRVLLIDDLLATGGTARAAANLLRRLGAEVVEIVFLIELT
                     ALEGRAKLEGYPVRSLIRF"
     gene            complement(14118..15029)
                     /locus_tag="PKK20_01835"
     CDS             complement(14118..15029)
                     /locus_tag="PKK20_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU98661.1"
                     /translation="MAKPPSPVPEVTVPRDEEALAERPADRSAYDSDSAIKLYLREIG
                     QVKLLTPQEEIELAARIKQGDKKAREHMIKANLRLVVKIAHDYDGLGLPLLDLINEGN
                     IGLMKAVERFDPAKGGKLSTYAAWWIKQSIKRALANQSKTIRLPVHLVDKISRMRRIA
                     TKLHEVYGREPTDEELGEELGVPSSRVAQLRSASVRPASLDAPIGDENSNTYAEVVED
                     ERAEDPYERLEEKTVNIMVDDFVRRLDQREQTILRYRFGLDGSSEKTLEEVGAKFGVT
                     RERIRQIQNIALAKLRRMIQKLEGKKR"
     gene            complement(15461..>15886)
                     /locus_tag="PKK20_01840"
     CDS             complement(15461..>15886)
                     /locus_tag="PKK20_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNU98662.1"
                     /translation="NNGWWGEGEIKFYMDGDTQWPTICGTGTEDYFCGSYNFDTKDDS
                     GKARYTVFSTPYSGLAQVLPPDQIYQAGQRFGLYRWHITDPIRFERDLRVTIQSLGWQ
                     EGGRYLQTEDDVAAVAFWYQTEPHAPFPPLPSRERLEPK"
BASE COUNT         2701 a         4876 c         5455 g         2854 t
ORIGIN      
        1 ggcgacgatg cgggatttga aggtgaatgt cgtgggatag agaaagtgac aggtttttaa
       61 tttgacgttc cgtcgagagt cctggcgacg agtcgcagtt gtcctgtgag ctgcggcatg
      121 caatcaactc gatgatgcgt tcggtgaatc tcgaagtggc ccggcccagc gtggacgagg
      181 cgaggcggcg gttgcttggg gctatcgacg ccgctcgcag gagccgtgtc cgcgtgctca
      241 aggtgatcca cggatggggt tcgagcggcg agggcggcgc gctgggaccg gccatccgca
      301 ggtccctccg gctccgggtc aaggagggcc gggcggtggc tgtcgtgccc ggggaacggt
      361 tctcgtcgga cactgccgag ggacgggaac ttctgcgccg ctacccctcg ctgcactcgg
      421 atcgggactt caaccgcgct aatcccggca tcacaatcgt cgagcttgcc ccttgaaccc
      481 gttgcggtcg ggaccggtgc ggagttgctt atcggttcca gccgagcgcc ggcgacttcg
      541 ggccggaggg aggggcctgt cttgagacaa agctcgtgat cgatcggggc ctctctcgcg
      601 accacggcaa agcccaaaac tccaaaatgc cggcagggct cccgatccga tcctcgatgc
      661 cggcgcatcg gatcacccgg cggttgtggt ggagatcggc gttcgagagc ggtgatgcgc
      721 cgaatccctc gaacggcgaa gccgctcggc gtgtcgtgcg tcaatccggg tagccgttgg
      781 gatgcgcctt atgccaggcc caggccgtgg ccacgatgtc ttcgagtttc gggtagcgcg
      841 gtttccagcc caactcgagt gcggcttttg cggccgaggc caccagacgc ggtggatcgc
      901 cggcccggcg cggtttctcg agggccggga tggattggcc gacgaccttt tcgcaggcct
      961 ggatcacctg gcgcacggag tagccttcgc cgttgccgag attgtagaag ccgttcttgc
     1021 ccggttcgag cgcccggatg tgggcgtcgg caagatcgac gatgtggatg tagtcgcgga
     1081 tgcaggtgcc gtcgggggtg ggatagtccg tgccgaaaat ctcgcagtgg ctggattgcc
     1141 cgagcggcac ccggagcaca ttcggtatca gatgggtctc gatccggtga tgttcgccga
     1201 accgggcgct ggctccggcg gcattgaaat agcgaaacgc gacgaactcg agtccgtgaa
     1261 tctgccgaaa ccattcgagc attttctcga acatgagctt cgattcgccg tacgggttga
     1321 tggggcgttg gggcagattc tcggtcatcg gaacccgctc gggcgggcca taggtggcgc
     1381 aggtggaact gaacacgaac ttgcgcaccc gactctcgac cgcggcgtcc aggaggttga
     1441 gtccgtaggc cacgttgttg cggaagtact tcgtcgggtt tgtcatggac tcgcccacga
     1501 gggcgttggc ggcgaaatgg agcacgccct cggcgtgcgt ctcgcgcagc gtacgcagga
     1561 tggcggcgcg atcgctcaga cacccgaaga tgaatcgcgc ccgcgaatcc acggccgcgc
     1621 ggtggccctc ggtgagattg tcgaacaccg tgaccgcatg gcctgcattg aggagttctt
     1681 cgacacagat cgacccgata tagccggcgc cgccggtgac cacgacattc atcgagggga
     1741 gagtctagcg aggcgcgcct cggagccaat cccaatcctc agcctcgaag gcaatcccaa
     1801 tgccctcaga gcagtgtgtt tggcggggcg aggcggcgcg gacgagtgtg gtgtcccaca
     1861 atgcgatggc gcttcgccac aagtgtcttt caccacccgc caggcgcgag cgaggcgcat
     1921 acccgagtcg attcccgccg acaatctcag gatgtgttgt tgaattcccg gttgacggcg
     1981 cgatggcttg gccgtcaaat ccggccatga acacattcca aatccacatc agtcgccgtc
     2041 gttttctcgc ccgttcggcc gttgcggctg gtttgcctct ctggtgcctt gacgagctgc
     2101 acagtcaggc agcccaaacc aggcctttgg ctgccaatga caagccgggc attggactca
     2161 tcgggtgcgg cggccagggg cggggcgact gcaactgggc gaggaactat gggacggtga
     2221 ttgcggtggc ggatgtggat gacgggcatg ctgaggaagc ccagaagcag ttcggcgccg
     2281 cgcacaggtt caaggatttc cgcaagctgc tggaacggga cgacattcat gtgatcatca
     2341 acggcacgcc cgatcactgg catacgctga tcaatatcgc gtccgcccgg gccggcaagg
     2401 acatttacag cgagaagccg ctgacgctca ccatagcgga gggcaagacg ctggtgcagg
     2461 agatccgcaa gcaccagcgc gttctgcagg tcggaagcca acagcgcagc gacgcccgtt
     2521 tccgcctggc gtgcgaactc gtgcgcaacg ggcgcatcgg gcgactcgag cacatggtcg
     2581 tcggactccc caccggtccg cgcgagggtc cgttctccgc cgtgcccgtg ccgcctgaac
     2641 tcgattggga ttactacctg ggtcaaacgc ccaaggtgga ttacaacggg cacaacagtc
     2701 actggaattt tcgctggtgg tatcagttct ccggggggca gataaccgat tggggcgcgc
     2761 atcacaatga tatcgcccag tggggtaacg gcaccgaacg gtccgggcca gtcgaggtgg
     2821 acgggaaggg gctggtcgag atgattcccg ggggttacga cgcgatggcc aagtaccgca
     2881 tcgactgcaa gtacgccaac ggtgtgacga tgacgatcgt cgacgaaggc accgtgaccg
     2941 accgcaacgt ggtcggcggc ggcaagagca cgcccaacgg cgtgcaattc atcgggaccg
     3001 acggttggat tttcgtgagc cggagcgaaa tcaaggcgaa caaacaggaa ctgatcgagg
     3061 aaccgctgcc gtccggcgcc acgcggctgc acaaaagcga caatcacatg ggcaatttct
     3121 tcgagtgtgt ccgctcccgg aaggacccga tctgcgatgt ggaaatcggg catcgctcga
     3181 tcagcatcgc ccatttgggc gtcatttcgg tgcgcatgaa acgccctctg cgctgggatc
     3241 catcgagaga acagttcatc gacgatgccg aagcggaccg ctggctcagc cgcgcgatgc
     3301 gcgccccgta cgacatgagc tttatctgac gcgcatattg acgcgacgcc agtgcacgag
     3361 cgatcggatt tcgcatgatg tctgccatgc ggactgaagc cgattgagat gcggacatcc
     3421 gacacagccg cttgggggcg agcgttcttg acaaggcgaa gtcgcgtcgc cgcgggagag
     3481 tgcctccacg cccgaggggc gacgtcctcg tcgcccgcga acgcattctg cgacccattg
     3541 gtcgcggacg aggacgtccc cgctcctcta ccgattcgat gaaggctgct tcttgcgcaa
     3601 gccggtcttt accgccgact cggatagttg tctgatgtcc gccgccgttc cctgtgttct
     3661 gttcgaggat gatcacctct tggtggtgaa caagccgccg ggctggaaca cgcacgcgcc
     3721 cagtccttac gccggcgagg gcatctacga ttggctgcga catcggagtc cgcgctgggc
     3781 gaacctggcg atcatccatc gtctggacaa agagacctcg ggcgttctgg tgttttcgaa
     3841 gagtgccctg gcgaaccgcg aactcacccg ccaattcacc gcgcgccagg tcaagaaaca
     3901 atacctcctg gttgccgatc gcccggtccc gcccgcgccg ttgcggttcg cgtcgcacct
     3961 ggttcgggat ggcgatcggt atcgcagcca gtcaacaggc ggctcgggca cgatggctga
     4021 gaccgttttt caacgggccg agcgcatggg cgaccgcacg acggtcgagg ccttcccgac
     4081 gaccgggcga acgcatcaaa tccgcgtgca cgccgctgcc gccggtttcc cgattctggg
     4141 tgacccgctt tacggcggga cccccgccag gcgcctgcat ttgcacgcgg cgtccgtcac
     4201 cctgcggcat ccggccacaa agcaggtcgt ggtctttcgc gcgccggcgg actgggatcg
     4261 ggagcccgca caggccctgc gcggcagctt catcgacccg aagcagacgg atgcatatcg
     4321 catcatccac ggcgctgctg acggctggcc gggatggtac gtggatcggc tgggtcggtt
     4381 tctgctctcg caaggcgagg ggcagctcac gggcggccaa aaagccgcgc tcgaacgttg
     4441 gatggccggg gtgtcggcgc gcggggtcta tcacaagcag cgcgaccggg gagtctgttc
     4501 atccgcgccg gaggatgtgg cgccgcggcg ggtgtttggc gaagcggcgt atggggatgt
     4561 gatggtgcgc gagaacggat tgcgctatgc ggtgcggttt gaccaaggct actcgattgg
     4621 tctgttcctc gatcaacgcg acaatcgccg acgcctgctg accgggcaca ttgcgggcgg
     4681 gctttcggcg tatccggacg gctgcaaggg catcgaggtg ctcaacctgt tcgcctacac
     4741 ctgcgccttc tcggtcgcag cggcccgggc gggcgcgcgg gtgaccagcg tcgatctggc
     4801 gcgcaagtac ctcgactggg gacggcgtca ttttgaggca aaccaattgg aggcgaatgc
     4861 gcatgatttc ctttacgggg acgcattcga ctggctgcgg cttctggcga aacgggcgcg
     4921 gcggttcaac ctggtgattg tcgatccgcc cacgttttcg acctcgaaga ccggcgggcg
     4981 atttcgggcc gaagcggact ggccgcggct ggcccgtgcc gctctggctg tgctcgcgcc
     5041 cggcggctgg ctttttgcgt caaccaatgc agcgcgggtc gaaccgggac ggtttctcga
     5101 ccaactcgtc gcggccgccg gcgaggctcg gcgccgcgtg ctcgacaggc attacgctcc
     5161 ccagccgcct gatttcccga tttgtcgcgc ggagcccgcg catctgaaga ccgtgtggct
     5221 gcggatcgag taggacgccc gcggatcatt tcgtcgttcg agcccgatag aaggcgcggg
     5281 gtcgattggt ggccgcgaca tctcggaagg tcgagcgtcc ggcagtgtcg gtgactgcga
     5341 tctgcgcatc ccagtcagcg aggtcgatcg aggactcgat tgcgtaggtg gtgtcggcgg
     5401 tgccgtgcag gtcgagtgtc gtgccggcct gtggcgacca ttcgcgaagt tcgaggctgg
     5461 gacgggacgg tttgagggcg ggaagccgca cggtgatttc gcccaacgca tcgctcttga
     5521 gcaggaagta atcgctttca agtccggacc ccaccgatcc ggcgatccac cctgagtatc
     5581 cgtggcgcgc cgcggacgcc gggtagtagt cccaccacag cagccaggcc tccggctcgg
     5641 gcgtgtctcc atccgcccgc cagagccgcg cctggagttc cggagcgcct gtgaggtcgc
     5701 gcacttcatg ccggatgcgc agccagtacc aggtgtttgg ttgccaggcg aacccggctc
     5761 ttgggccctg cggcatcgag tcgccgctca gcccgatgcc ccgcccagac gccgcgttcg
     5821 aatgcagttg gcaggccagg cccgtgcgcg ccgccggatc gacgccgagc gcaaggccgc
     5881 cccacgggcc gtcgtcaggc gtcgggccga ccgttcggat gcgggcaagg atctcgcacg
     5941 attcgaggcc cggccgcggc tgttcgagga gcagccgacg cgtggcgcct ggcgccgggg
     6001 acaaccgcaa gagcccatcg gcaacgcgaa agaaatcggc gtcgtcgccg gtgatcgacc
     6061 actcggggcc cagggcaccg ctgaagtcct cctgccatcc gcgcagcgtt tggcccgctt
     6121 ccgctggaag gccgagccgc gtgacggtca cccgtcgcac cgtgagggcg ggcgcttgga
     6181 tggcgatgcg gaagtccgct ccgccgttct gcgaattgag gaagcgggat tcgaggaggc
     6241 ggaatctggc cttcttccat tgtctcgtgt tgttgagggt gacgctggtc ttgcttgccg
     6301 tgtaggcgcc attgaacggg gcgttggggt ccgggccgtc gtattcgatg cggaacgagc
     6361 ccgtgccggc gtcgaaatac tcgacgtcga catccaccag catcgagtca gcccacttga
     6421 aggagtcgtg gacctggaag tagatgtact tgccctgatg caccgtggac acgctctccc
     6481 ggcattcgtg gcctcccgca gtcgcggggc gcgtgacgcc atcggcatgg tcgaactgga
     6541 tcaggccttt ggactcgttg gtggccccga gactcgtggt gacggaccga acctccgagt
     6601 acggaccgcg ggggcggggc ggcttgatgc ccgctttgaa catgtccgcg aacttgcggt
     6661 tcagatcgat gtattggcgt ccgtactcct tcgaggcggc gatgtccgtg ccttcgtggt
     6721 attcgttcca tgtttcgaca tggaccagca gcgaggggtt gcggaggaaa cgcgtccagt
     6781 tgcgttcgaa gaaggcgcca tcttcgcgtg gaaccacgag cggctcgcgg cccgggacgg
     6841 cggagtggtc gtagccgggg ccgagggcgg ccacgctcag gttcttgagc gaaattgcgc
     6901 cgccccaggc gtaggtgtta tccgcccgaa cgttccacga ggtctcgcgc acgatgtacg
     6961 gacgccgccc gccaaagtcg cgcggaaagg actcagcgac gaagtcgatg caggtctggt
     7021 cgtgggctgc ggcaaagccg gccgcgtaga ggaagaccac gggtcgatcg tcgatcatcg
     7081 cccaatgccg cggggggatg agcgagaaga aatcgcggat gctctcgtaa aaccattggc
     7141 gtccgtaatc ggttgtgagg tcgatgcgtc gcccggccgc gttgtactcg agcgtcgagg
     7201 tgtcgtagaa gagacccacg cgcggcggtt gcttgccctc ggcgcgcaga gcatcgaggg
     7261 cggcgaccag cggaggcagg ccggcaaagc tccacggttg ctcgagaacg ggtttgccgg
     7321 ccacgcgttg ggacggttcg ccccagtaga ccggcagcag cacgtcgact ccggcggcca
     7381 tcatgtcgag caactgctgc cgatgccagg atgtggactt gtaggagaag ccggtcagcg
     7441 tgggcggatg atcggtcagg gcgtcgctgc cgtcgccgtt cctgatgtgt gcgccgctgt
     7501 agacatcgta ccagtagaaa taggaggtca agaccacgcg gtcttgggcg gtgaagctga
     7561 cggcctgggc gaagtcgtcc ggttcgaggt tcacatgcgg tccgatgggc ggattgatct
     7621 cggcggccat cgcgcctgaa gatgtcagac cggcgcacaa ggcgagcccg agcccgagga
     7681 tcgaaaggca gactgtattc atgcagagtt ttcgcatctg gaatccttga cagatcgagt
     7741 ttctgtgttg cgttggcggg atcaggcatt ctcggcttcg ccgaagtcat cactcgatga
     7801 agcatgcgga tcagccccgg cgcgatcaag tcaagttccg cgcaggaaga agttgcgttg
     7861 tttgcggctg atcgggaggc ccagacgcgc catcacctcg agcatgtcct cccagacctt
     7921 gcgcttttcg gccagggcct ggttgcgcag gaggtaggct gggtgataag tcggcatcag
     7981 gggaatgccg tgaaactcga gccagtggcc gcgcagcttc gtgatgccct ccatcttgcc
     8041 catcaagccc tcgacggctg tgccgcccag tgccaccaac accttcggct ggatgatgtc
     8101 gatctgcgcc agcaggtgcg gcaggcaggt ggccatctcg atcggcgtcg gcttgcgatt
     8161 cccgctgctc tggccggggg tgtccggacg gcatttcaga atgttcgcaa tgtagaccgt
     8221 ctcgcgcgac aggcccatcg tctgaatgat gcgagtgagc aactggccgg ccttgcccac
     8281 aaacggaatg ccctgcgcgt cttcatccgc tccgggcgcc tcgcccacaa acatgagttc
     8341 cgcgcgggcg ctgcccgttc caaacaccac cgtgcgccgg ctcctggcca aatgctcgca
     8401 cttccggcat gggaggacgc gggcccgcaa cgcctcgaga gcggtttctt tgtcgtcggg
     8461 cgagcgcatg gaagagtcgg gacgatctgg cttgagacca gcctggtccg cggccgcgct
     8521 aggggccggg gttggaacgg agcgtggcgt ggccgcggat gacaaaggcg cccgccgcga
     8581 cggtgcgggc gggcgcggac acgactggtt cagggcggcc catgtgttgg catggaccgg
     8641 gacgaaccgg acgccccggg ctcgcaggtc ttcgagatgg ccgatggcgg cgtcgatcag
     8701 ttgactcaac cccgcatggc tctcggcgct cacgacaggc ccttgacttc ccagccggcg
     8761 cggtactggc gccgcacgaa cttgttggcc tcggccacgt tcttgaactg cacgttggcc
     8821 gcgtcccatt cgagtgtggt ctgcgggaag tgcgtggcga cgccgcccag caagaccgcc
     8881 tcggtgagcg gtccggagta gtcgaagttt gccgaagggg tgccgttgcc caggcaggcc
     8941 tcgacccatt catgccagtg attggcgccg gacacccggg ggaccgggta atcttcgaat
     9001 tgtttctcgg gcaggagctg aggcggtccg atgtgcggca gcaacaacac gcctttggtg
     9061 ccgatgaaga tcgacccctg atccggcacg ggttgctggc cgatcagcgc ctgcacgtcc
     9121 ttgggcggcc tttggtcgcc gtcgtaccag gtcactttga ccgtgctgcc ttcgctgaac
     9181 ggggtgctgg gaaagacgta atgaatcacg gcatcgaccg accagttcca cgcgttgggg
     9241 ggcggcccct cggaacgtac cgaaagtggc gcggtcagcg cgagcgcctt gaacaccgga
     9301 tcgtagatgt ggcagcccat gtcaccgaag gtgccggtgc cgaagtccag gcgtttgcgc
     9361 cagttgcccg gatggtaata gccccctccg atgtacggac gcgacgccgc cacccccagc
     9421 cagaggtccc agtcgaatcc cgccggcacg gggtcggtgc ggtccggtcg aggggtcatg
     9481 tcgccccact tcttgccgct ccaggtgtgg acctcgcgga ccttgccgat ggcgaggtcc
     9541 tggatcagcc gcaccgccag gcgatactcg gaggccgaat gaatctggat gcccatctgg
     9601 gtggccagtc tctgttgtcg ggcgaagagg gtcagttgcc gcgcctcata gacgtcgtgg
     9661 gtcaacggtt tctcgccgta catgtgcttg cccaactgca tcgcggacat gccgatgggc
     9721 gcgtgcatgt gatcgggcgt cgagacgttg accgaatcca gacccttgac ctcgtccagg
     9781 agcttgcgcc aatcctgata gaccttgagt tccgggaatt tcgccttgag cccatccgcg
     9841 cggggcaggt cgacgtcggc caccgcgacc aatcggacct tgggatggcg cgtgatggac
     9901 tcgaggtcgg cggccgccat gccgctggcg ccgaagctgg cgtgccgcaa gacttcgttg
     9961 ggggaagcgg accaggaata acgggcaacg aacggcgcgg ccaatccggc tgcgcccact
    10021 tgccgcagaa aggcgcggcg tgaactgcga aaagtcgatg tcatgcgcga aactctaatc
    10081 cgcaggctcg ctgagggcaa ttgcggattc accggggcag acacctgctt tgagcatcac
    10141 tgttcagcag ggttgaccgc gaaacacgcc aaacacgcga aaaggatggc agagcgcttg
    10201 tccgccgcac cgccggcttc gtgtatttcg tgtgttccgt gggccattcg ggcttgatac
    10261 gccgcccgca cgctccggcc ctgcacgtgg ttgctatcgg cgctaatccg cttcttcgcg
    10321 ccaacgcgcg acgagttgat gggcaatgcc caggtgatcg agcacgcgcg cgaccacggt
    10381 gtcggccacg tcctcggcgg ttctgggccg gctgtaaaac gacgggtttg cgggcaggag
    10441 aatcgcgccg gccagggcga gcagttcgag gttcttgatt tggacgaggt tcaggggcgt
    10501 ttcgcggggg acgaggatga gcttgcggtg ttctttgagc accacgtcgg ccgtccgcag
    10561 cagcagatcg ccgctgagtc catgcgcgat gcgcccgagg gtgcccatgc tgcacggaat
    10621 gacgaccatg gcgtcggggg cgttcgaccc gctggcgaat ggggcgttca tgtcatcgag
    10681 ggcatgagag accacccctt cggcgaggcg cagaccaccg ggcaactcct cggcgacgac
    10741 ttcggcggcg tagcggctca tgagcagatg cacctggtgt tggcggacgt ccagacagtc
    10801 caagagccgc tgggcgtaga ccgcgcccga ggcgcccgtg atggcaacga caacgcgcaa
    10861 tgctacagtt tcacatcctc ggcgcggacg tcgaggcact cgtcctgatc ctcccaggcg
    10921 actgcgggat agacttcgag cagtttgggg acaagttggt ggccggcgaa tgccagcaat
    10981 gtctccgcct tggccgcggc gtcatcgagc gccgcatcgt aatcccccac gcgctttcgc
    11041 tctccgtcgt cccaatgcgc gacggcatgg acgggcgcgt attcctcggc ccaaccgcgg
    11101 atcagggggc gcaggtcctc gggcagatcg tcgaacggca cggccgtgct gttgcagtgc
    11161 agacagacca ggccggattc gaggacgtca cggctcagca tcggcacgag cggcgcgcgg
    11221 caacagggcg cgtcgggata gttggcggga gacatcgcca gccgtttgag ccaaatctgc
    11281 cggtcgtgtc ccacatgcgc agcgagtcgg tacgccgcca gaagagggtg ggacagccgc
    11341 cagccgcgtc cctgaagttg ccccagggtt tgcgccatcc accgcgccaa cgtcagatcg
    11401 tcgaagtcgt gaaaaacccg gctgtgctcg gtccacaagg cgtccagcgg gttctccggt
    11461 tcatgagcca tgatggattc agactaatgg gcagggcaaa agttcaaagc gaaaagacgg
    11521 gtgagtcgtg gtgaggatcg tggaggcgtt gacattcggc acttgaatgc gcacgcgtct
    11581 ggaccgcgtg cgatttcact tgagccggcg cacgatggcg tcggcatcga gtgtgggagc
    11641 gaaggcatag cgatcgcgca ttgcgcagaa gctgacgtca tcgcgcaatt cgggtcgagc
    11701 cagcaggcgg cgtccgttgc ggccggagcc caatccggcg gcgacgtcat tggcgtgcct
    11761 gcaccaggct tcgagagcga gtagcacgga atcgagcact tcgccttccc tgtagagttc
    11821 ccagacggcc tcgcacagcg cgcctgcggc cagcgcgtcc tcgaaagcgg cccgctcgat
    11881 cgtcccgccg cacaccacca gcaggtggcg cggctggacg tggcgcaggg cttcgacaac
    11941 ggcactgaga ttgaggaatg ctcccaccca cacgtgcgcg gccggccggc acgcctgcaa
    12001 ggcacgcgtg ccgttcgtgg tggtcatcac gatcgtgcgt ccccgcaccc gctccggcac
    12061 gaactcgcgc ggcgagttgc ccagatcgaa ctcgactccg ccggtgaggg cagccccgat
    12121 tcggaggccg tcgcgttcgc ccgccaggag gacatccggg tcgcgttcct tccacgccag
    12181 ggcctccggg attgtggcga ccggcacgac ggcctgggcg ccatgggcca gcgcggtgac
    12241 catcgagctg gtggcgcgca ggatatcaaa cacgacgcac gccgtctcgc tcaagtcgcg
    12301 ctgtgcgagg gcggcaaact cggcgggtgt gaagaggacc tcgatcgagg tgttcatggc
    12361 ttagggtctg tgtaaaaact ggttccgatc tttctcgagg ggatttggct cgccggcgag
    12421 gcgcgacgaa ggagcatagc cttgacctct gtgactgaag agcaacgaag ccggcgagcc
    12481 aaagcccccc agccctccgg ggcggggcgg caccgggcca tctgcgtcgt tcctcggtcg
    12541 gtcgagtatc tctggggata cgggatacac tcccaccctc gggcttcgca tctggcccgc
    12601 cggcgctccc gccaaaaccg gaagtaattt ttgcacagac ctttaccgtg aacgctgtcg
    12661 gtcgacagtt cggatgtaat ggaacaggta ttgttgggcg tagcccgcgc gcggcccgaa
    12721 gtgcgtgccg gcgaagcgtc ggagcccgga cggcgtgacg cggcgtcgcg ggaagtagag
    12781 ccgccgcagc actctctcga tccacacatc aatggggaag gcctgcggga atccatacgc
    12841 gaagagcagc acgcaatccg cgatcttgcg tccgactccg cgcagagcca tgaggacctc
    12901 gcgggcctct tcgagctcga gggacgggag cgtcgccaga ttcgggcggc ccgcggcgac
    12961 gctttgggcg gccgcccgaa gcgagggcgc gcggaatccg agcttgcagg cgcggagttc
    13021 ggcttcggtg cactgcgcca ggcgttcggc tgtggggaat gcgaactcgg gggcatgtcc
    13081 tcgagggacc ggcaccggct cgccgtaacg ggcgcagagc agtccgatca tctggcgaat
    13141 ctgcacgatc tgtttcgtgg cggagagaat gaaggaggcg aggcattccc acgggtcctg
    13201 gcgcaagagg cgcagaccgg ggaaatgccg cacggcgtca gcgaggatcg ggtcattcgg
    13261 gaagcttgcc agcacggtgt cgaggcgctc gccgacctgg aggtaggttg cgagccagtc
    13321 ccagtccgga acgggcgcgg gcgtctctgc gcggattccc tccgcgcacg gctcgagacg
    13381 tgtccaacgc cggcccacca cgccctcgaa gcccgcgccc gccggacgcc agcggaaggc
    13441 ctgtccggaa ttgagcgttg cggccaggtc gtaggggcgg acgcggaacg tggtgtgctc
    13501 gacgcgggtc agcctgggcg aggacgggcg attcgggaag ttcgttgaca tccagctctc
    13561 taaaaccgga tgagggagcg cacggggtag ccttcgagct tggcgcggcc ttcgagtgcg
    13621 gtcagttcga tcaggaatac gatctcgaca acctcggcgc ccaggcggcg cagcaggttg
    13681 gccgccgccc gcgcggttcc gcccgtggcg agcaggtcat cgatcagcag aacgcggctg
    13741 ccttcgggga cggcatcggc gtggatggcg atggtgttgg aatcgtattc gagttcgtag
    13801 ctttcttcga gcgtgcggta ggggagcttg ccgcgcttgc ggatcgggac gcagccggcg
    13861 ccaagtttca gggcggcggc ggaggcgaaa aggaaacctc gggcgtcgat gcccgcgacg
    13921 gcgtggatgg agccgggtgg gtggcccgcg atcagcctat cgacggttgc ggcgaacagg
    13981 cgtgcgttgc ccagcaaggg tgtgatgtct ttgaactgga tgcccggttt gggaaaatcc
    14041 ggaacattgc gaatggcctg ctcgagttcg gccggcgagg ggtcttgtgg agaattcatc
    14101 gaagacgtgt catcctgtca gcgtttcttt ccctcgagct tctggatcat gcggcgcagt
    14161 ttggcgaggg cgatgttctg gatttggcgg atgcgttctc gagtgacgcc gaacttggcg
    14221 cccacttcct cgagtgtttt ctcgctgctg ccgtcgagtc cgaaccggta gcgcaggatc
    14281 gtctgctcgc gttggtccag ccgccgcacg aaatcgtcga ccatgatgtt caccgtcttt
    14341 tcctcgagcc gctcgtacgg atcttcggcg cgctcgtcct cgacgacttc ggcgtaggtg
    14401 ttcgagttct cgtcgccgat aggggcatcg agcgaggcgg gtcggaccga cgcgcttcgc
    14461 agttgggcca cccgagacga gggaacgccc aactcctcgc ccagctcctc gtcggtcggc
    14521 tcgcgcccgt acacctcgtg cagcttcgtc gcgatccgcc gcatgcggga aatcttgtcc
    14581 acgaggtgga cggggagccg gatggttttg gactggttgg cgagggcgcg tttgatcgac
    14641 tgcttgatcc accaggcggc gtaggtggac agtttgccgc ccttggcggg gtcgaagcgc
    14701 tcgacggcct tcatcagtcc gatgttgccc tcgttgatca aatcgaggag gggcaggccc
    14761 agcccgtcgt agtcgtgcgc gatcttgacg accaggcgca ggttggcctt gatcatgtgc
    14821 tcgcgcgcct tcttgtctcc ctgcttgatg cgcgccgcca gttcaatctc ctcctgcggc
    14881 gtcaggagct tgacctgtcc gatctcccgc aagtaaagct tgatcgccga gtcgctgtcg
    14941 tacgccgaac ggtccgcggg ccgctcggcc agcgcctctt cgtcccgggg cacggtcacc
    15001 tccggaaccg gcgacggagg cttcgccatc tcgcccgcgc ggggtcgaac gccccgtgcg
    15061 ccctcgggcg aacgagcaga ccgcgtccgg gccggcgttt tgggtcggcg gacacgtctc
    15121 tgagacgatc gtccctgcgg ctggcgcttt gctgtcatag tccgacgcgc cgcaagagtg
    15181 tgaagtccgc cgtcgttgcc gcaagcaaat taggcagcaa atgaggcgcg atccggtgca
    15241 tccggtcgtt gttggtattc tgtcgcccgg tgaaggcacc gggtcgacaa cggatcgaga
    15301 ttgccggtgt aggccgcgtg ccctgcctgc gccgcaaagc gcagcggcct gtctgccgtg
    15361 tcgggcacgg cacaggcagg cgcggcaggc aggccccacg cggcgcatac tcgattccca
    15421 ccgacaacct ccagatgcac tgcccgcgtg cggacacggc tcacttcggt tcgagtcgct
    15481 cccgggaggg cagaggcgga aacggcgcgt ggggctcggt ctggtaccag aaagcgacgg
    15541 cggcgacatc gtcttccgtc tgcaagtacc ggccgccttc ctgccagccg agcgactgga
    15601 tcgtgacccg caaatcccgc tcgaaccgga tggggtccgt gatgtgccac cggtagaggc
    15661 cgaagcgctg gcccgcttga tagatttggt cgggcggcag cacctgggcc aggcccgagt
    15721 acggcgtcga gaacacggtg taacgcgcct ttccactgtc gtccttggtg tcgaaattgt
    15781 acgacccgca gaagtaatcc tccgtgccgg tcccgcagat cgtcggccac tgcgtgtcgc
    15841 cgtccatgta gaacttgatc tcgccctcgc cccaccagcc gttgtt
//
LOCUS       DAOQRO010000024        15601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2866_length_15601_cov_5.252605, whole genome shotgun sequence.
ACCESSION   DAOQRO010000024 DAOQRO010000000
VERSION     DAOQRO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_15601_cov_5.252605"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(427..2901)
                     /locus_tag="PKK20_01845"
     CDS             complement(427..2901)
                     /locus_tag="PKK20_01845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNU98663.1"
                     /translation="MPLPAKPIHAVQVRVLVEFALRRGDLGGEGDFVGPRRALAGTRG
                     HQRLQRSRAAGYQKEVSLHRDVDDGEWVLRVQGRLDGLWIEDGRTRIEEIKTIQGARE
                     RRAHPLHWAQAKCYGFMYAEAHGLESIDIQLTYLDVDTDEVTEYREGFGRAELAAFFF
                     DLTAVYLEWLRARRQWCRERDESIGALRFPFPRYRAGQRAIAVAGYRAVARGGRLFVE
                     APTGIGKTVAALFSAVKAMGQGKIDRLFYLTARTVGRVVAEQACGELRRAGLRLRVVT
                     LTAREKICTHEGGVCDPENCPMARGYYDRHRAAMRAALAHEAITRFVIEEASRAHQVC
                     PFELSLDVSSWVDAVICDYNYVFDPQVYLRRHFLDEPGDCVFLVDEAHNLVDRARAMF
                     SADLDTREIREVRRAIRERAPRCAKALNRLAAAIAKLGGPAAHADNSIEPASPHLQPD
                     LFSPRDAVSRERSGPDSGLKRADGADARDGARTSREFPRALEPLVEEALGQAEEWLVR
                     NEPAGFRESLIELYFRLHAFRRTAGLYDAHYATIIEPGASVRVRLFCLDPSFLLQQAL
                     ERGRSALFFSATLTPVDYYRSLLGGSVEDPILRLPSPFPSGNLAVLTHDRIRTVLKAR
                     TQTLEEVVQAVGALVRGRVGNYLVYLPSYQYLSAVQERFQARFPDATILMQRPGMSEP
                     EREAFLGAFAVDGGTVRVGFAVLGGVFGEGIDLVGERLIGVAVVGVGLPQLCIERDLI
                     RDYFEERTGAGFDYAYTFPGMNRVLQAVGRVIRSETDRGVILLIDTRFAESRYRALFP
                     PGWNPVTVRSVGAIQEAVLRFWEPAV"
     gene            3148..4926
                     /locus_tag="PKK20_01850"
     CDS             3148..4926
                     /locus_tag="PKK20_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98664.1"
                     /translation="MRTLLLILRSSVLRSARLRSLSCLLPIAIGLASADSRLHAQSTA
                     LRSALEPPDATIDAPCVTPPPESFFQMVSNRIARADARRGRPRRRASEAPPPEAASVA
                     ESARSRVADELAIYRTFYAKHIDVQGLPVAAHADVADLALQRTYQIVTGMLAGRPDIL
                     AAMVSNQIYLIVIGKNQVYTDMPEYRHHPNPEFQNERVRGTGGNPTSFGEENLLSLPI
                     DRYDDESIAVHEFCHTIDDILGRIDPTWQERRMAAYRNAMNKKLYEQTYAASNPAEYW
                     AEIAQAFFDCNRVNNWNHGPVGNRAQLLAHDPAGFALCQEVFKSAPDRDWRYTWLQRL
                     PMVSAPPRLDKFKDIDPWYTKFTWAREFTIVGRTASDDALLHANETTRRLFAYRHDFL
                     KALINHGLKLVVLGPGEQLADLPEWKRTSEKPSIDGASRGPTCDPNLNLLVVAQEDVL
                     AGAASPWSTGSPVIAVLAEAAYRVAGLRPVIPDYHGNQQYELRVKRLDVEFDQAVTAL
                     FNQARAADAWADTPAAQDKFAYWCAGVLAYFDASGPHSAPRGSTTPVNTRERLATYDP
                     GLFAIVHETMAYQDKVDWRYQARRSN"
     assembly_gap    5167..5266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5565..7070
                     /locus_tag="PKK20_01855"
     CDS             5565..7070
                     /locus_tag="PKK20_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNU98665.1"
                     /translation="MKRREFLQTTATFSLAAASGVLSRQAFAAQPLDMRIGASDLRVQ
                     PVLMYGLTQRVPGRSWRPWGDIQTEEGVTEELGRIDRELWQLKNGADFGVSFIPTARV
                     SAKPQVEALKSAQADALLVYASGGGTDLFEALAGVGKPMVIFVRDRSGPYYLWHEIVD
                     SRFLRSHTDSVKQTNAGLEDVVVDDQGEILWRLRGLYGLKNTTGRRIVCVGGPSGWSC
                     PQAPERARQRFQLDMITAPIPEVNARIETARKDAAAVDRSRSEARAYLDARGVKSQIP
                     EESVAEAFLLKGVFHQLMREHDACAVTTNGCMASYAGIMPCMTLTLINDDGYMAYCES
                     DFVNIPAGILLHFIAGRPTYFCNPTIPHRGRMLFAHCTAPRRMDGQKLEPVTLVTHYE
                     SDHGAATHVHFRRGQKVTIIKPDFEAKHWLALTGTTVDAPFLATCRGQIEVALDAATQ
                     DVIRNMRGFHCQIAYGDWTREIVYAAKKVGIEVQQLPAGQGRKAMGSHLNS"
     gene            complement(7144..7500)
                     /locus_tag="PKK20_01860"
     CDS             complement(7144..7500)
                     /locus_tag="PKK20_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98666.1"
                     /translation="MSGSSSPHGRHLAEYLARIQTKSEVYPYLDGTWFRTFDYRRWEP
                     WGGSGDVGWGAWCIEAGWAQAWGSAIMALREKGTALRDLTVHSAVARHQDAVQTAMTR
                     NAGGPWEAPTKEEPGL"
     gene            complement(7484..9133)
                     /locus_tag="PKK20_01865"
     CDS             complement(7484..9133)
                     /locus_tag="PKK20_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNU98667.1"
                     /translation="MTETRHVLRSELPGEVRDARLSIARNEWGSFQVLVRADAPVSGL
                     RLDAGNLKGPDNRAADAIRLVLYRQHQLFLDTGTHRNADFKPDGYPDPLIPFDHPVAG
                     ERLRDSRLKAIPFELPAGETHGFWVDVFASTNAAAGEYHGTCQVMAGQEQVAEIPFHL
                     TVWDFALPSVPALETEFGSPAGRLKAYYRERAKAGREAEPTDWAAVETECAQVLCDHR
                     LNAVPPGELITPVAQSDGSFRIPPQHVAALRRFIERYHVNAVQIPHPSRVVKDPEAER
                     DRLRAWLAAFDIAARELNQAHLVFFTYLRDEPNTLDDYRYVQTWGRAVREARSVVKVL
                     VVEQTWTAPGQEGADSAWGDLYGAVDIWCPLFSLHEQESAARRQALGETIWTYTALCQ
                     GKPTPWWHIDYPLLHYRVPTWIAWRHHMRGLLYWGGLSYWGPVDDPWVEAPYYAGNGA
                     PQQGRKGVVFYGEGSLVYPGRAVGCEGVVPTIRLKALRDAIEDYDYLALLEREGRASE
                     ADAIVAPLVSSFFAWDKRPAAYERARSALAGLIVTGRERQF"
     gene            9636..13274
                     /locus_tag="PKK20_01870"
     CDS             9636..13274
                     /locus_tag="PKK20_01870"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98668.1"
                     /translation="MKGNRWLLAEALLVGLLWAGHPHLRAAASTPGAASADIEFLRRL
                     TLDVVSASRVKPGERVGDSPRIEGWLMAATEVGRQPDVWGTLCALHLGALSEAAAERA
                     LATVADAVRRRTIVFKGAVRHVPTDLDASPTSAWERTAVRLNTYQNGAYWHTPSGWLV
                     AALRKRDLRLAAQVLADYVDHARRGDFRRGSDQEAPWESIHPGRYVQNGVYMTSVALP
                     GSVLFSGSLEPANRTGQSSPAGRQAVEEEFAARAAGIGSAAAVNQLVATIEFEPREAR
                     VVRLVIRRPHAGEPCLDELEVYGPGCVTNLALAERGAVARASSLLPGYAIHAVAHLND
                     GLYGNDHSWIAATAGEEWAEIELPEPARIARVVLSRDRDGRFTDRQILEAEVQLSLDR
                     RTWRTVGTLRRSANELRLPMPTLTLPLGELPKPTWAGAVDYAFLRERDTWSRMDATDY
                     LSPLVNDRPAWPGGPPYWGRLARLDPLERTLTQYSDMIERLGLIGLDVGAEREELAAL
                     RREAEDSALAGSDDLFLRARHAKRRLFFRDPRLGALERVLFAKHHPLQPSHNYSEHLD
                     SLFAPGGGICVLHVPRDAEGRLNPARAEVETLFDGSGGIVRHPVADYDARTVYFAYRP
                     DKPEVEGWQSYWHLYSVRADGSGLRQLTEGPFHDFDPVPLPDGALGFMSTRCQGRYLC
                     WEPQAYVLYRMQPDGMNLRRLSYANISEWDPVVMRDGRILWTRSEYLDKGADFGHTLW
                     AIRPDGTHPELVFGNNTPYCYGHAREVPGSQELVCTLISHGDHQGPIALINPARGLFD
                     TAALTSITPDTRPQYQMDRSHHETFRDPEAVSSDHFLVTHNPGRQSHWGLYVIDRFGN
                     RELLYLDPAISSKRPSLLRAQPRPPLLFEAQNPDLAREGLGQFVVQDVYEGLGPTVAR
                     GQAKYLQVSQEVPSPLERLADGQYRNAHPGYPDYYATPIHLVTGPRQDYSTRTANALE
                     PHAFRAGSAARAGDGTVTITEHGGWPSYVAKAVLGTVRVAEDGSANFTAPAGKVLYFH
                     LLDEDFNELQRMRSVVQLQPGEQRSCIGCHEDRHAAPLRPTRRPLAAAAQTLTPPPWG
                     GLAFDYERVVQPVLDAHCVRCHDTQSEAPLDLRGTRDGERVPASYRSLIGGGWVHYFD
                     WHYGARHFKAEPLTFGTRRSRLFTALAGEQHKTVALEPADMRALKAWIDLNCPLWPDY
                     RHRSERPL"
     gene            13362..14558
                     /locus_tag="PKK20_01875"
     CDS             13362..14558
                     /locus_tag="PKK20_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98669.1"
                     /translation="MAIADLEPKVGGVTPSAPEAVVVVRGLTKVFKDFWGRPKARAVD
                     AVDFEVRRGEVFGLLGPNGSGKSTTVKLLLGLLHPTRGHIEVFGHSPRHVKTKARIGY
                     LPEESYLYRYLDSAETLDFFGNLFELTSKERRNRSEQLLEMVGLSQVRRRTVGEFSKG
                     MQRRIGLAQALINDPDMIILDEPTSGLDPIGCREVKDLILALAQRGKTVILSSHLLAD
                     VEDVCDRVVVYYGGRIQAMGTLKELLATPDAVRITSPLLPRETLERVLEIIRRDVGAN
                     RVEIDTPTQNLESYFLKVVEQAKQRETTSGATSGARVAAYLRGEADQPSSVERRLERL
                     SLPQTPAPTPEVKSPAAPSVDETRLAALAHGEEKSPPPVKVQTPPPADLDRANEKLSS
                     LLGDKP"
     gene            14555..>15601
                     /locus_tag="PKK20_01880"
     CDS             14555..>15601
                     /locus_tag="PKK20_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98670.1"
                     /translation="MRPILAVAGLTFRAAFRFRLVPVLSVLLVASVFLLPAFIKHTGK
                     AQEFVQIVLTYTLGLTTAVLGLTTLWLACGTLAGDIEDCQMQVVAVKPIARWQIWLGK
                     WLGIMMLNGLMLAVCAGAIYAQLQWRARGLSQEQLEVLNNEVLVARGSFKESLPDLEP
                     VVEEEYQKRLESPAFAEMPRSEARRIIREQLKAGLQVVPPAYTRVWRIDLGSVKSALA
                     GRPLYLRIKFFVSEKSASGTYWGLWEVGPPESPRRVQDVKNHAAETFYEFSVPPDLFD
                     AEGLLTVEYTNQNDTAVLFPLDEGFEVLYRAGGFGLNYVRGVAILFCWLALLAALGLA
                     AASFLSFPVAAFLAL"
BASE COUNT         2829 a         5189 c         4840 g         2643 t
ORIGIN      
        1 ggggacgcga ctctacagac acgggatgga tgagcagggg ggaggctgcc cagacacaac
       61 catcgcgtgc atggttgact tgttacatgg ggtttactcc agacatgatc ccttgtaaca
      121 agataggcgt ggctcgatct gcaacaacct acaggcgcaa gtggcccagc ggacaaacga
      181 ccgtcattcg agtgccgcaa acccttgcgc ccaaggtgct ggagtccccc gtcatctgct
      241 cgggtgagcc ggccatatgt ctatcgcggc gcgggcaacc tgctcaactc cgtcagcagc
      301 cgtcaatacg ccagtcgcaa cgcgcttctg cgagtgccca tcagcctgtc aaccgcttcg
      361 cttcaaacag tccgtaccgg gtcgcgaggg aagtcgagct gacagaatga gccgtcgtac
      421 gggccgttag acagcaggtt cccagaacct caggaccgcc tcctgaatcg cgcccacact
      481 tcggaccgtc acgggattcc agcccggagg aaacagcgca cggtatcgcg actccgcaaa
      541 ccgggtgtcg ataagcagga tcaccccccg atccgtttcc gagcgaatga cacggcccac
      601 ggcctgcagg acacggttca tgccgggaaa ggtgtaggca tagtcgaatc ctgcgcccgt
      661 cctttcctcg aagtagtcgc ggatcagatc ccgctcgata cacagttgag gcagacccac
      721 acccacaacc gcaaccccaa tcaaccgctc tcccaccagg tcaatgccct cgccaaacac
      781 accgcccaag accgcgaatc caacccggac cgttcctccg tccacggcga acgctccgag
      841 gaaggcctcg cgttccggtt cgctcatccc gggacgctgc atcagaatcg tggcgtccgg
      901 aaagcgcgcc tggaatcgct cctgcacggc gctgagatac tggtaggaag ggagatagac
      961 gaggtagttc ccgacgcgcc cccgaaccag cgccccaacg gcttgcacca cctcctcgag
     1021 ggtctgggta cgggccttca acaccgtgcg aatgcggtca tgtgtcagca cggccaggtt
     1081 cccggacgga aatggcgagg gcaacctcag gatcgggtct tccacgctcc cgcccagcag
     1141 ggaccgatag tagtccactg gcgtgagcgt cgccgaaaag aacaaggccg accggccccg
     1201 ctcgagggcc tgttgcaaca aaaaggacgg gtccagacag aacaaccgca cgcgcacgga
     1261 ggctcccggc tcgatgatgg tcgcgtagtg cgcatcgtaa agcccagcgg tgcgccggaa
     1321 cgcgtgcaga cggaaataca actcgatcag actttctcgg aatccagcag gttcgtttcg
     1381 aacaagccac tcctcggcct gccccagcgc ctcctcgacc agcggctcga gcgcccgcgg
     1441 aaactcgcgg ctcgtgcgcg ccccgtctct cgcatccgcg ccgtcagccc gcttcaaccc
     1501 ggaatcgggg ccgctgcgct ccctcgacac cgcgtctctg ggtgagaaga ggtccggctg
     1561 gaggtgggga ctggccggct cgatcgagtt gtcagcgtgg gcagccgggc cgcccagttt
     1621 cgcgatggcc gcggcgagcc ggttcaaggc tttggcacag cgtggcgctc tttcccggat
     1681 cgcgcgcctg acctcgcgga tctcacgggt gtcgaggtcc gccgagaaca tcgcccgggc
     1741 gcgatcgacc agattgtggg cctcatccac gaggaaaacg cagtcgcccg gttcatcgag
     1801 gaagtggcgg cgcagataga cctggggatc gaagacatag ttgtaatcgc agatcaccgc
     1861 atcgacccac gacgagacat ccagagagag ctcgaacgga cagacctggt gcgcccgact
     1921 ggcttcctca atcacgaagc gtgtgatcgc ttcatgcgcc agggcggcgc gcatcgcggc
     1981 ccgatgccga tcgtaataac cccgggccat cggacagttc tccggatcgc acaccccgcc
     2041 ctcgtgagtg cagatcttct cgcgagcggt cagtgttacg acgcgaagcc gcaggccggc
     2101 gcggcgcagt tcgccgcacg cctgctccgc aaccacccgc ccgaccgtcc gcgccgtcag
     2161 atagaaaagc cgatcgatct tcccttgccc catcgccttc accgcggaaa agagcgccgc
     2221 gacggtcttg ccgatgcccg tcggcgcttc gacgaacagg cgccctccgc gcgccacggc
     2281 gcggtaccca gccaccgcga tggcgcgctg gcccgcccga tagcgcggaa aggggaagcg
     2341 gagggcgcca atggactcgt cgcgttcccg acaccactgg cggcgcgcgc gcagccactc
     2401 gagatagacc gccgtcagat cgaaaaagaa cgccgccaat tccgccctgc cgaagccctc
     2461 ccgatactcg gtcacctcgt ccgtgtcgac atccagatag gtcaactgga tgtcgatcga
     2521 ctccaaccca tgggcttcgg catacatgaa accataacac ttcgcctgag cccaatgcag
     2581 gggatgcgca cgccgttccc gcgcgccttg aatcgtcttg atctcctcga ttcgcgttct
     2641 cccgtcctcg atccacaagc cgtcgagacg cccctgcacg cgcaggaccc attcgccgtc
     2701 gtccacgtcg cgatgcagcg aaacctcctt ttggtaaccc gccgcacggc tccgctgaag
     2761 ccgttgatgc ccgcgcgtcc cggccaacgc ccgcctcggc cccacaaaat ccccctcgcc
     2821 gccaagatcg ccccgtcgca gggcgaactc gaccaggacg cgaacctgga ccgcatggat
     2881 gggtttggcg ggcagtggca tttcccgagg aacttcgact ggctgacctt gcatgaacgc
     2941 cccggggaaa gccatatcct tttgcgcaac ggacgcgctc gagttgcatc tgaaagatgt
     3001 ttgtactctg tcgcccgcca aacagggtct gcaactctca tcgccgggga cgaggctgtc
     3061 cccactccaa cgccagcctg tgcgtaaaca gagcacaccc ggccggttct tgctgacgcc
     3121 cgagccgctc gcttgtactc tcggcccatg cgcacgttgc tcctgatcct tcgctcgagc
     3181 gttctccgca gtgccaggct gcgctccttg tcgtgccttc tcccgatcgc catcgggttg
     3241 gcaagtgccg actcccgtct ccacgcccaa tccacggcct tacggtccgc gctcgaaccg
     3301 ccggacgcca caatcgacgc cccctgcgtg acgccgcccc cggagtcctt ctttcaaatg
     3361 gtgtccaacc gcatcgcccg ggccgacgcc cgccgcgggc gcccgcgccg tcgagcaagc
     3421 gaagcgcctc cgccagaagc ggcaagcgtt gccgagagcg cccgatcccg cgtcgcggac
     3481 gagttggcca tctaccggac gttttatgcg aagcacatcg atgtgcaggg gctgccggtc
     3541 gccgcgcatg cggatgtggc cgatctggcg ttgcagcgaa cctaccagat cgtcaccggc
     3601 atgctggccg ggcgccccga cattctggcc gccatggtca gcaaccaaat ctacctgatc
     3661 gtcatcggca aaaaccaggt ctacacggac atgcccgaat accggcatca tcccaatccc
     3721 gagttccaaa acgagcgcgt ccgaggcaca ggcggcaacc cgaccagttt cggcgaggag
     3781 aacctcctca gcctgcccat tgaccgctac gacgacgaga gcattgccgt gcatgaattc
     3841 tgccacacga tcgacgacat cctgggccgg atcgatccga cctggcagga gcgccgcatg
     3901 gccgcctacc gcaacgccat gaacaagaag ctctatgagc aaacgtacgc agccagcaat
     3961 cccgccgaat actgggcgga gattgcgcag gccttcttcg actgcaaccg ggtcaacaat
     4021 tggaaccacg gcccggtcgg taaccgcgcc caattgctgg cccacgatcc cgccggattc
     4081 gccctgtgcc aggaggtgtt caaatcggct cccgaccggg actggcgtta cacctggctg
     4141 cagaggctgc caatggtctc cgcgccgccg cgcctcgaca agttcaagga catcgacccc
     4201 tggtacacga agttcacctg ggcgcgcgag ttcaccatcg tgggacggac agcgagcgac
     4261 gacgccctgc tgcacgccaa cgaaaccacc cgccggctgt tcgcctaccg ccacgacttc
     4321 ctcaaagccc tgatcaacca tggcctcaaa ctcgtcgtgc tcggccccgg cgagcaactg
     4381 gccgatctgc cggaatggaa gcggacctcc gagaagccgt cgatcgatgg cgcgtctcgt
     4441 gggccgacct gcgatccgaa tctgaatctg ctcgtcgtgg cacaggaaga cgtgctggcc
     4501 ggcgcggcgt caccctggtc cacaggcagc cccgtcatcg cagtcctggc cgaggccgcc
     4561 tatcgagtcg caggcttgcg tcccgtgatc cccgattacc acggcaacca gcagtacgaa
     4621 ctgcgcgtga aacgcttgga tgtggaattc gaccaggccg tcacggcgct cttcaaccag
     4681 gccagagccg cggacgcgtg ggccgacacc ccggcggcgc aggacaagtt cgcctactgg
     4741 tgcgccggcg tgctcgccta tttcgacgca tccgggccgc actcagcgcc gcggggcagc
     4801 acgacgcccg tgaacacgcg cgaacgactc gccacctacg atcccggcct cttcgcgatc
     4861 gtccacgaga ccatggctta tcaagacaaa gtggattggc gctaccaagc ccgccgctcg
     4921 aattagtgtg gtgtctcaca attggctggt gcttcgccgc aaagattctt cgcctcccgc
     4981 gggcgcgagg gacgcgcata ccacactggc ccggaccctt cacgcgctcc acgccgagcc
     5041 ggagccgtag ccgacatcgc cggtggcgtc cgaactccga actcccatct ccgaactccc
     5101 atctccgaac gcccatctcc gaactcccat ctccgaactc ccatctccga actcccatct
     5161 ccgaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccca tctccgaact
     5281 cccatctccg aactcccatc tccgaactcc catctccgaa cgcccatctc cgaacgccca
     5341 tctccgaacg cccatctccg aactcccatc tccgaacttc catctccgaa ctcccatctc
     5401 cgaactccca tctccgaacg cccatctccg aactcccatc tccgaactcc catctccgaa
     5461 ctcccatctc cgaactcccc ggcaaagcgc ttgccgaagc agggcggggc gccgtatcat
     5521 agcggcatag ccacaactgc aaaccactgc aacacactat gaacatgaaa cgacgcgaat
     5581 tcttgcaaac aacggcgacg ttcagtctgg ccgcggcgag cggtgtcctg agtcgccagg
     5641 cgttcgccgc ccaaccgctc gatatgcgga tcggggcgag cgatctgcgc gtgcagccgg
     5701 tgctcatgta cggcctgact caacgggtgc cgggacggag ctggcgcccg tggggcgaca
     5761 tccagaccga ggaaggcgtg accgaggagt tgggacgcat cgaccgtgaa ttgtggcaac
     5821 tcaagaacgg agccgatttc ggagtctcgt tcatccccac ggcgcgggtc agcgcaaagc
     5881 cccaggtcga ggcgctcaag agcgcccagg ccgacgcatt gctggtctac gcgtccggcg
     5941 gcggcacgga ccttttcgag gccctggcgg gtgtgggaaa accgatggtg atcttcgtcc
     6001 gcgaccggtc cggcccctac tatctctggc acgaaatcgt cgactcgcgt ttcctgcgca
     6061 gccacaccga ctcggtcaag caaaccaacg ccggcctcga agacgttgtg gtggacgatc
     6121 agggggaaat cctctggcgg ctgcgggggc tgtatggatt gaagaacacg accggacgcc
     6181 gaatcgtctg tgtcggcggt cccagcggct ggtcctgtcc ccaggcgccc gagcgcgccc
     6241 gccagcggtt ccagctcgac atgatcaccg cgccaatccc cgaggtgaat gcccgcatcg
     6301 agaccgcgcg aaaggacgcg gcggccgtgg atcgcagccg gtccgaggcc cgtgcctatc
     6361 tcgatgcccg aggcgtcaag tcacagattc ccgaggaatc tgtggccgag gcgtttctgc
     6421 tcaagggggt cttccaccaa ctgatgcgcg agcacgacgc ctgcgccgtg accaccaacg
     6481 gttgcatggc cagctacgcc ggcatcatgc cctgcatgac actgacgctc atcaacgacg
     6541 acggctatat ggcctactgc gagtccgatt tcgtcaatat ccccgccggc atcctgctcc
     6601 atttcatcgc cggccgcccc acctacttct gcaatcccac catcccccac cgcgggcgca
     6661 tgctcttcgc ccattgcacc gcgccccggc gcatggatgg tcaaaagctt gagccggtga
     6721 cgctcgtcac gcactacgaa tcggaccacg gcgcggcaac tcacgtccac ttccgccgtg
     6781 ggcagaaggt cacgatcatc aagccggact tcgaggccaa gcattggctc gccctgaccg
     6841 gcacaaccgt cgacgccccc ttcctggcaa cctgccgcgg ccagatcgaa gtcgcgctcg
     6901 atgccgccac ccaggacgtc atccgcaata tgcgcggatt ccattgccag atcgcctacg
     6961 gcgactggac gcgggagatc gtctacgcag caaagaaggt cggcatcgaa gtccagcaat
     7021 tgccagcggg ccaggggaga aaggcaatgg ggtcgcatct aaatagttga caaatgcttg
     7081 gccaccacgt cgcccgacgg cgttgcgcgg atgacgagca ggtcgagagg ggctgcggcc
     7141 acgtcagagt ccaggctcct cttttgtcgg cgcttcccag gggccgccgg cattgcgcgt
     7201 catggcggtt tgcaccgcat cctgatgccg ggcgaccgcg ctgtgaacgg tcagatccct
     7261 taaagccgtc cctttttcgc gcaaagccat gatcgccgaa ccccacgctt gcgcccaacc
     7321 ggcttcgatg caccaggcgc cccagccgac gtcgcccgag ccgccccaag gctcccagcg
     7381 cctgtaatcg aacgtccgga accaggtgcc gtccaggtag gggtacacct ccgatttagt
     7441 ctggatgcgg gcaaggtact ccgccaggtg tctgccatgc gggctagaac tgccgctcac
     7501 ggcccgtgac gatcaacccc gccagagcgc tgcgcgccct ttcataggct gcggggcgct
     7561 tgtcccaggc gaagaacgaa ctgaccaacg gcgccacgat cgcatccgcc tcggacgccc
     7621 gtccttcgcg ctcgaggagg gccaggtaat cgtaatcctc gatcgcgtcg cgcagcgcct
     7681 tgagtcgaat cgttggcaca acgccttcgc aacccacggc ccgcccaggg tagaccagcg
     7741 agccctctcc gtaaaagacg actcccttcc tgccctgctg tggcgctccg ttccctgcgt
     7801 agtaaggagc ctccacccaa ggatcgtcca ccggtcccca gtaggacagc ccgccccaat
     7861 agagaagccc ccgcatgtga tggcgccaag cgatccaggt cggcacgcgg tagtgaagca
     7921 atggatagtc gatatgccac cagggcgtcg gcttgccctg gcacaaagcc gtgtaagtcc
     7981 agatggtttc gcccagggcc tggcgccgcg cggcactctc ctgctcgtgc aaagagaaga
     8041 gcgggcacca gatgtccacg gctccgtaaa gatcgcccca ggcgctgtcg gccccttcct
     8101 gacccggagc ggtccaggtc tgctcgacca ccagcacttt cactacagac cttgcctcgc
     8161 ggaccgcgcg tccccaagtc tgcacgtacc ggtagtcgtc caacgtgttc ggttcgtccc
     8221 gcaggtacgt gaaaaaaacc agatgcgcct ggttcaattc ccgggcggcg atgtcgaacg
     8281 cggccagcca ggcgcgcaga cggtcgcgtt cggcctccgg gtccttgacg acgcgagagg
     8341 ggtggggaat ctgcaccgcg ttgacgtggt aacgctcgat gaatcggcgc aacgccgcca
     8401 cgtgctgcgg cggtattcga aacgaaccgt cggactgcgc gacgggagtg atcaattccc
     8461 ccggcggcac cgcgttcagt cgatgatcgc agagaacctg agcgcattcg gtctcgactg
     8521 ccgcccagtc ggtcggttcg gcctcacgtc cggccttggc acgctcgcgg tagtaggctt
     8581 tgaggcgtcc cgcgggcgag ccgaactcgg tctccagcgc cgggacgctc ggcagcgcaa
     8641 agtcccagac ggtgaggtgg aatgggatct ccgcgacctg ttcctgcccg gccattacct
     8701 ggcaggtgcc gtggtattca cccgctgcgg cgtttgtgga tgcaaagacg tccacccaga
     8761 agccgtgggt ctccccggcg ggaagctcga aaggaatcgc cttgagccgt gaatcgcgca
     8821 gcctctcgcc cgccaccgga tggtcgaaag ggatcagcgg gtccggataa ccgtccggtt
     8881 tgaaatccgc gttgcgatgc gtccccgtat cgaggaagag ctggtgctgc cggtagagca
     8941 ccaggcggat ggcatcggct gcacgattat cgggtccctt gagattaccc gcgtcgaggc
     9001 gaagaccaga gaccggtgca tccgcccgga ccagaacctg gaaactgccc cattcgttgc
     9061 gggcgatcga gagcctggca tcgcgcacct cgcccggcaa ctcgctgcgg agcacgtgtc
     9121 gggtctcggt caccacccaa cagtgaggcg gagccgcaag gagccggggg cacaagggga
     9181 ttgagagcag gaacgcgaga gggccaagcc agggtcgata tgcagagagg agtctcatgg
     9241 agctgccgtc cagtctattt gtgagttctg gctgggagcg tacccttgaa tccggtgtca
     9301 tcgcaagcaa gcagttgttc cccaatgaac acgcttccca tcatcgtaac ttccccaggc
     9361 cagcccccgc cagcatcggg tgtctataat aatgaacctg tcactttatt ccgtcgccgc
     9421 gccgctcaag gtcaggaggt ctgagcctga ggttctgact gattcgcctt gtgattcgca
     9481 ttgaaatgcc tgtgcagtcc gggcaccctg acacgagtat tccatgatgc cccgcgcggg
     9541 ttgagacgcc gggggttgtg gaaagaccgc gtctcgagac aaagctcgtc cggcttgggc
     9601 gcgaggcgcg ccgcaaaggc gctgacccaa tgagaatgaa ggggaatcgt tggctacttg
     9661 cagaggcgct cctcgtcggg ctcctgtggg caggtcatcc gcacctacgg gctgccgcgt
     9721 caacgccggg ggctgcctct gccgacatcg agttcctgcg gaggctgacc cttgacgtgg
     9781 tgtcggcttc gcgggtcaag ccgggggaac gggtcggcga ttcgccgcgg atcgaaggct
     9841 ggctgatggc ggcgacggaa gtgggacggc agccggacgt ctggggaacg ctctgcgcgc
     9901 tgcacctggg ggctctgtcg gaagccgcgg ctgagcgcgc cctggcaaca gtggccgacg
     9961 ccgtgcgacg ccgaacgatt gtctttaagg gcgccgttcg gcatgtgcca accgaccttg
    10021 acgcatcgcc cacctcggca tgggaacgga ctgctgtgcg cctcaacaca taccagaacg
    10081 gcgcgtattg gcacacgccc tcgggctggc tggttgcagc cttgcgaaag cgagacctgc
    10141 ggctcgcagc ccaagtgctc gccgactatg tcgatcatgc gcgtcggggc gatttccgcc
    10201 gaggatcgga ccaagaggcc ccgtgggaga gcatccaccc cggccgctac gttcaaaacg
    10261 gcgtctacat gacttcggtg gcgctgccgg ggagcgtact gttttccggc agcctcgagc
    10321 cggcgaaccg gacgggccaa tcgagccccg ccggtcgcca ggcagtggaa gaggagtttg
    10381 cggcgcgcgc ggcgggaatc ggctcggcgg cggcggtcaa ccaattggtc gcgaccatcg
    10441 agttcgagcc gcgggaagcg cgggttgtgc ggctggtcat acgccgtccg cacgcgggcg
    10501 aaccgtgcct ggatgaactc gaggtgtacg ggccgggctg cgtcaccaac ctggccctgg
    10561 ccgaacgcgg cgccgtggcg cgggcatcgt cgctgcttcc gggctacgcg attcatgccg
    10621 tggcgcatct gaacgatggt ctgtacggca acgaccacag ttggattgcg gccacggcgg
    10681 gcgaggagtg ggccgaaatc gagttacccg agccggcccg gatcgcgcgc gtggtccttt
    10741 cgcgcgaccg cgacggaagg ttcacggaca ggcagattct cgaagcggag gtgcagcttt
    10801 cgcttgaccg gcgaacctgg cggacggtcg gcacgttgag gcgttcggcg aacgaactgc
    10861 gtttgccgat gccgacactg accttgccgc tgggagagtt accgaagccg acctgggcgg
    10921 gtgcggtcga ctacgcgttc ttgcgggagc gggacacgtg gagccgaatg gatgcaacgg
    10981 actacctctc cccgctggtg aacgatcggc cggcctggcc gggcggcccg ccctattggg
    11041 ggcggctggc gcggcttgat ccgctcgaac gcacgctgac gcagtactct gacatgatcg
    11101 agcggctggg gctgatcggg ctcgatgtgg gcgcagagcg ggaggaactg gccgcgctgc
    11161 gcagagaagc agaggattcg gcgctcgcgg gttcggatga tctgttcctg cgcgcgcggc
    11221 acgccaagcg tcggcttttc ttccgtgatc cgcggctcgg cgcgctcgag cgcgtgctgt
    11281 ttgcaaaaca ccatccactc caaccgtcgc acaactacag cgagcatttg gattccctct
    11341 ttgcgcccgg cgggggaatc tgtgtgctgc atgtcccgcg cgacgccgag gggcgtttga
    11401 acccggcgcg ggcggaggtg gagacgcttt tcgacggcag cggggggatt gtgcgccacc
    11461 cggtagccga ttacgacgcg cggaccgttt actttgcgta tcgtccggac aagccggagg
    11521 tcgagggatg gcaatcgtat tggcatttgt attcggtgcg agccgacggc agcgggctgc
    11581 gccagttgac cgagggtccg ttccacgact ttgatccggt cccgctgccc gacggcgcgc
    11641 tggggttcat gtccacgcgc tgccaggggc ggtatctctg ttgggagccg caggcgtacg
    11701 tgttgtaccg gatgcaaccg gacggcatga atctgcgccg gctttcctac gcgaacatca
    11761 gcgagtggga tccggtcgtg atgcgcgacg ggcgcattct ctggacccgg tcagagtatt
    11821 tggacaaggg tgcggacttt gggcacaccc tttgggcgat tcggcccgac gggactcatc
    11881 ccgagctggt ttttgggaac aacacgccct attgctacgg gcatgcgcgc gaggtgcccg
    11941 gcagccagga actggtctgc acgctcattt cccacggcga tcatcagggg ccgattgcgc
    12001 tgatcaatcc ggcccggggg ctgttcgaca cagccgccct gacgagcatc acccccgaca
    12061 cccggccgca gtaccaaatg gaccggtctc accacgagac cttccgggat cccgaagcgg
    12121 tctcgagcga ccactttctg gtcacccaca atccggggcg gcaatcgcac tggggcctct
    12181 acgtcatcga ccgattcggc aaccgggaac tgctctatct cgatccggcg atcagcagca
    12241 agcgcccgtc cctgctgcgc gcgcagccgc gtccgcccct gttgtttgaa gcgcagaatc
    12301 cggatcttgc gcgggagggg cttgggcagt ttgtggtcca ggacgtgtac gaggggcttg
    12361 gtccaacggt cgctcgcggc caggcgaagt acctccaggt ttcccaggaa gtgccttccc
    12421 ctttggaacg gctggccgac ggccagtatc gcaacgcgca tccgggttac ccggactatt
    12481 acgcaacccc gattcacctg gtgaccggcc cgcgccagga ttactcgacg cggaccgcca
    12541 acgcgctgga gccgcatgcc tttcgggcgg gcagcgcggc ccgagccggg gacgggacgg
    12601 tgacgatcac cgaacacggc ggctggccca gttacgttgc caaggccgtg ctcggcacgg
    12661 tgcgcgtcgc cgaggacggc tcggccaact tcacggcgcc ggccggcaaa gtgctttatt
    12721 tccacctgct ggacgaggac tttaacgagc tgcagcgcat gcgcagcgtg gtccaactgc
    12781 agccgggcga acagcgcagt tgcatcggct gccacgagga ccggcatgcc gcgccgctgc
    12841 gcccgacccg acgcccgctc gccgccgcgg cgcagacgct cactccgccg ccttggggcg
    12901 ggttggcatt cgattacgaa cgcgtggttc agccggtgct cgatgcgcac tgcgtccgct
    12961 gccacgacac ccaatccgag gcgccgctcg atctgcgggg cacgcgcgat ggggaacggg
    13021 tgccggcgtc gtatcgctcg ctgatcgggg gcggatgggt gcattacttt gactggcact
    13081 atggggcccg ccatttcaag gccgaaccgc tcacgttcgg cacgcgccgg agccgccttt
    13141 ttacggcgct ggccggagag cagcacaaga ccgttgcgct cgaacccgcg gatatgcggg
    13201 cgctcaaggc ctggatcgac ctcaactgcc ctctgtggcc ggactaccgc catcgcagcg
    13261 agcgtccgct gtagaatcct cgaccgatca gacgcgctgt tctgtgccgc aaaacgccca
    13321 gttgtcggcg cggaggtgct ttgctaaagt gacacatcct tatggcaatt gctgatttgg
    13381 aaccgaaagt cggcggcgtc accccgtccg cgccggaagc ggtggtggtg gtgcgcggtc
    13441 tgacgaaggt tttcaaagat ttctggggcc ggcccaaggc gcgcgcggtc gatgccgtgg
    13501 acttcgaagt ccggcggggc gaggtgttcg gtttgctggg tcccaacgga tcgggcaagt
    13561 cgacgaccgt gaaactcctg ctggggcttc tgcatcccac acggggccac atcgaggtgt
    13621 ttggtcattc cccgcgccac gtgaaaacga aggcgcggat cggttacttg cctgaggaat
    13681 cgtatctata ccgttatctc gattccgcgg agacgctcga tttcttcggc aatctcttcg
    13741 aactgacgtc gaaggaaagg cggaaccgaa gcgagcaact gctcgagatg gtcgggctga
    13801 gccaggtgcg tcggcggacg gtgggcgagt tttccaaggg catgcaacgt cgcatcggcc
    13861 ttgcccaggc gctgatcaac gatcccgaca tgatcatcct cgacgagccc acgtcgggac
    13921 tcgatccgat cggctgccgt gaggtgaagg acctgatcct tgcgttggcg caacgcggga
    13981 agacggtgat tctgagcagc catttgctgg cggacgtcga ggacgtttgc gatcgtgtcg
    14041 tggtgtacta cggggggagg attcaggcca tgggcacgct caaggaactc ctggccacgc
    14101 ccgacgccgt gcgcatcaca tccccactat tgccgcgcga gactctcgaa cgggtcctgg
    14161 agatcatccg acgcgatgtt ggggccaacc gggtcgagat cgacacgccg acccagaatc
    14221 tggaaagtta cttcctcaag gttgtcgagc aggccaagca gcgcgagacg acatcgggcg
    14281 caacctcggg tgcccgtgtg gcggcgtatc tgcggggcga agccgatcag ccttcgagcg
    14341 tcgagcgccg actcgaacgt ctcagcctgc ctcaaacccc cgcgccaacc cctgaagtca
    14401 agagtcccgc ggcgccgtct gtggacgaga ccaggctggc ggccttggcg catggagagg
    14461 agaagagccc gccgccggtc aaggttcaga cgccgccgcc ggccgacctc gatcgggcga
    14521 acgagaagct ctcgagcctg ctgggggaca agccatgagg ccgatcttgg ctgtggcggg
    14581 gctgacgttt cgggcggcct ttcgtttccg actggtgccc gtgctgtcgg tgcttttggt
    14641 ggcttcggtt ttcctgctgc cggcgttcat caagcacacg ggaaaggcgc aggagtttgt
    14701 gcagatcgtg ctcacgtaca cgctggggct gacgacggca gtgctcggct tgaccacgct
    14761 ttggctcgcg tgcggcactt tggcggggga catcgaggac tgccagatgc aggtggtcgc
    14821 cgtgaaaccg attgcgcgct ggcaaatctg gctgggcaag tggcttggca tcatgatgct
    14881 caacgggctg atgctcgcgg tgtgcgccgg cgcgatttac gcccaattgc agtggcgggc
    14941 gcgcggcctc tcgcaggagc agctcgaggt cctcaacaac gaagtcctgg tcgcccgcgg
    15001 ttcgttcaag gagagtctgc ccgacctcga acccgtcgtc gaagaggagt accagaagcg
    15061 gctcgagagc ccggcgttcg cggaaatgcc gcgctccgaa gcccgccgca tcattcgcga
    15121 gcagctcaag gccggactcc aggtcgtccc gcccgcatac acacgggtat ggcgtatcga
    15181 cctcggctcg gtgaagagcg cgctggccgg acgcccgctg tatctgagga tcaagttctt
    15241 cgtgtccgag aagagcgcgt ccggcaccta ctggggcctc tgggaagtcg gtccgcccga
    15301 atcgccgcgg cgcgtccagg atgtgaagaa tcatgcggcg gagaccttct acgaattctc
    15361 ggtgccgccg gacctgttcg atgccgaggg tctgctcacg gtggaataca caaaccagaa
    15421 cgacaccgcc gtgctgttcc cgctggacga aggcttcgag gtactctacc gcgcgggcgg
    15481 gttcgggctg aactacgtgc gcggcgtggc gatcctattc tgctggctgg ctctgctcgc
    15541 cgctctcggg ctggccgcgg cgagcttcct atcgtttccg gtcgccgcct ttctcgccct
    15601 g
//
LOCUS       DAOQRO010000025        15204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2975_length_15204_cov_4.532246, whole genome shotgun sequence.
ACCESSION   DAOQRO010000025 DAOQRO010000000
VERSION     DAOQRO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15204
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_15204_cov_4.532246"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1588)
                     /locus_tag="PKK20_01885"
     CDS             complement(44..1588)
                     /locus_tag="PKK20_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98671.1"
                     /translation="MVSAIDYRIRFHRALWYGLILLAALSLSVFLASDQFMVAFVVGG
                     ILWLATLPYHAHMAMLVTVTTFSSALILPFIPGRPYMWEGAALLAWTGVIVLHMVRRG
                     SPDLGATLRAHRWIFIGSIIYCVVLVLTMMVRGFGLRILGSGQAGGRLYLQQLLCAIF
                     PLLFVMLQSNERLLVKLLTIQWLLTGTYLIADFAFTFGGGLVNALYFVELPVDAASFE
                     FQSMRFGIRRFQSFALVSMGLFFVVLVYNNTKDFVGRRAVWLLPVTVLVLLVGMLSGH
                     RVVLVTTGFVMLFCVFAQRFFTVRNQLIALLILIPSFAVLYAFADRMPLAAQRAVAFL
                     PGISIDNAARVDAIGTMTARRTMFRVGLEMIPQYLWVGRGFARYLDEYSRAFDPTGTM
                     MHINQGVFYNGFVGLMVNTGVFGTAGMLLFMYGGTRVAWRIIQHLRRHGCEDNFSRAC
                     SVVSGIWMGSAISFLFLHGDSEYAMKTFSLQAGVLIMANRFLEQRLVAASAESSAEPG
                     AVPSQA"
     gene            1833..3068
                     /locus_tag="PKK20_01890"
     CDS             1833..3068
                     /locus_tag="PKK20_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU98672.1"
                     /translation="MPTTRDRQRWRVLYCVDTLSAGGAERQTVELVTRLNLERFAPRL
                     ICLHGKAYGASLHFAPHLDRANVPWRVLDVRWSPPGLLAGWARLIAAVWRMRPALLHA
                     ISHHSNHIARLGRALMPPRLRLVTAVRTEYQPRQLFYERIEHRLAQRVVCNSPHLERQ
                     LREHSRIPARKLVCIPNGLDCERFGRNPDPGLRARLAPNASCVVAMLGRITKQKSPHL
                     LALALGELKRRQELPGGVRVFVAGEHESDEIQTQLDEAIRRHGLGGVITQLPQTNQPE
                     ALLHAADFTVLASLWEGLPNAVLESLAAGRPALVSEAANASGVIEDGVTGWVARTDDV
                     PHLATRLGEVFRLSPERLEGMRPACRDRAGMYSMTRMVQRYEELYEELLSGASPAPGT
                     RAEPQPRRAPTTKAKRVDR"
     gene            complement(2966..3922)
                     /locus_tag="PKK20_01895"
     CDS             complement(2966..3922)
                     /locus_tag="PKK20_01895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU98673.1"
                     /translation="MKLRPESVTEWLAPRRPGPPLVSIVLPTYNRAGLLPRSVDSILG
                     QTERDLELLIVDDGSGDETREYAAGMREQDPRVGYMREQNLGLPRALNNGFRVARGEF
                     WTWTSDDNRYFPEALERLSAYLRGHPEVGLVYGDMIQRREQGVVFNPPVYRDTFWRNS
                     LFGAAFLYRAAAARQAGEYDASLRMVEDYDFMLRLSYVAEVGHWPYIIYEYGEHEASL
                     TSTRPMEHLQAIGRMFEKHRRLGLASRRDLSDLAVQISGTSRRRGQCWNGVRFGIKAI
                     CVWPFNWRAYRSTLFAFVVGARRGWGSARVPGAGLAPDKSSS"
     gene            complement(3933..4826)
                     /locus_tag="PKK20_01900"
     CDS             complement(3933..4826)
                     /locus_tag="PKK20_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU98674.1"
                     /translation="MEEFYARTLQRLIDEGVLRRDTELLVVCGSDQDRDVLRHVGFTR
                     VTISNIEPGVTEDLVAPYTWSLQDAEALGFPDGAFDFVVAHEGLHHCHSPHRAMLEMY
                     RVARRGILVFEPRDTFLVRLGVRLDFGQEYEVAAVAGNRMTSGGVDNTAIPNFVYRWS
                     EREVEKVIRAWEPRGLPRIRYFYSLRVPHGRIAAMKNRWASRTLRALLPLVRLFTKVF
                     PRQSNCFAWLVTKIEVPRDLHPWLEMADGKPVFSVAWARRHYGEFKRPGAGSDGTVPP
                     GGGGCESGGMNPRGIARDRVG"
     gene            complement(4847..5890)
                     /locus_tag="PKK20_01905"
     CDS             complement(4847..5890)
                     /locus_tag="PKK20_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNU98675.1"
                     /translation="MARPLRVDVADAWYHVMNRGHRGGALFLDEADRRRFLGVVAELP
                     ERFELEVHAFVLMPNHYHVLVRTREANLSQAIHWLNVSYAVKFNWAHRCRGTVFQGRF
                     KGVLIQEEDKAVEVARYVHLNPVRITGLGLNTSDQRRARVLGCENPGEELVRRRLQTL
                     REYQWSSWRVYMGLEPNAGWLETGVIERACGGRGRAERRAALKAYTEQPIRQGGLESP
                     WEGMVAGVVLGDIEYARKLLSGRAVNEEEQTAARRMRRRVGWEELVGAAEKMRGEPWE
                     RWAERHGDWGRDGVMYVAVRHGGVRLAEAVRRVGIKYQAGAQAVKRFARRLGEDAERK
                     RFVAKLRSELSTI"
     gene            6047..7993
                     /gene="asnB"
                     /locus_tag="PKK20_01910"
     CDS             6047..7993
                     /gene="asnB"
                     /locus_tag="PKK20_01910"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNU98676.1"
                     /translation="MCGICGIWHFASNEPVDAELLARMTRQFVHRSPDEEGFHCEGGV
                     GLGFRRLGDIDLATSHQPMPNEDGSLRLVANGEIYNSQELRDRLAPRHTLRTQGDIET
                     ILHAYEEHGVDCVHQFRGMFAFAIWDRAQQRMTLAVDRFGKKPLYYALDGKKLVFGSE
                     LKCLRQHPGIALDLDYPALDEYLSTGYVRAPRSIYRTIRKLPPGHTLTVAANGGSRLA
                     EYWHPRLCAPPEHSQASIEELAAELRERLLEAVRLRMISDVPLGAFLSGGIDSSIVVG
                     LMSRLSSRPVKTFSIGFEGDPNDESPYARAVAVHCRTDHVHEIVKPDVVDILPKLVRH
                     YDEPFADNSMIPTYYVSVMARKAVTIALSGDGGDEVFAGYQWYRRAYRHSRLQAFIPG
                     LLRPAVAVLARVFPPDSTVHRYLGVIDQPPTCWGLAQEYFNRGRRARLYNDWTRTQVG
                     ESDADAGREAIFESVKGLGYLSQLQYADLVGFLPGDGLVKVDRASTLASLEVRSPLLD
                     HRVFEFMARVPPHYKLDGRESKVLLKRAAGEVLPPSILTRRKRGFDLPAGEWLRGPLR
                     PMLEDLLLAPGARTATFLDQTGVRRLAKEHADGRMRHDSRLWALLCLELWLREFPQPG
                     AETGASRHTSPESRGNPPRPAAPL"
     gene            8012..11788
                     /gene="metH"
                     /locus_tag="PKK20_01915"
     CDS             8012..11788
                     /gene="metH"
                     /locus_tag="PKK20_01915"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNU98677.1"
                     /translation="MTEHSSHQNSRAADLRRVLKERIAIIDGAMGTMIQARQLDEAGY
                     RGERFRDWTCQVKGNNDLLNLTQPQLIREIHTAYLDAGADLIETNTFNSTSISLADYG
                     LEAFAHELNLVGARLARAAADAYMAEHPERLCLVLGALGPTSKTASLSPDVHNPAYRA
                     VTFRQLTDAYADQARGLLEGGVDALLVETVFDTLNSKAALFAIAECFEQIGCRVPVMV
                     SFTITDKSGRTLSGQTVEGYWNSVSHFPLLSIGINCALGAKQMRPYIEELSRIAPVYL
                     SCYPNAGLPNAFGGFDETPEQMSRDLRDFAVNGWLNIVGGCCGSTPDHIRAIAAAVRG
                     VPPRVPPTVEPWLRLSGLEALSLRPDSNFMNIGERANVTGSPKFAKLILAGKFDEALT
                     IVRQQVENGAQVIDVNMDEGMLDSEQAMVHFLHLIAAEPDIARVPIMIDSSKWSVIEA
                     GLQCLQGKGIVNSISLKEGEDEFLSHARRIRWYGAAVVVMAFDERGQADRYERKIEVC
                     ARAYRLLTERVGFPPEDIIFDPNILTVATGIEEHNRYALDFIEATRWIKQNLPRAKVS
                     GGVSNISFSFRGNNLVREAMHSAFLYHAIRAGLDMGIVNPGMLEVYEEIPSDLRERVE
                     DVLFDRRPDATERLVALAESVKQKDKAEVVDDAWRRGSIEERLHHALVKGIVDFVEVD
                     VEEARRKYGRPLDVIEGPLMAGMNIVGDLFGAGKMFLPQVVKSARVMKKAVAYLTPFL
                     EAEKQAGARAQGKVLMATVKGDVHDIGKNIVGVVLGCNNYQVIDLGVMVPCDTILKTA
                     REQRVDAIGLSGLITPSLDEMVHVARELEREGFRIPLLIGGATTSKAHTAVKIAPAFS
                     QPVVHVLDASRAVPVVSHLLRPDQKGAFVEQVRAEYERLIAQHRSARPRLLTIEEARA
                     RAPRLQYDDIPQPEFIGIRTIGTQPPSPPSDRPASTSQDAPPRVDHRAITLADLVPYI
                     DWSPFFHAWELRGRYPAILDHPRHGEQARQLFEDGQKMLERIVSERLLEARGVYGFFP
                     AARAGDDVQIFVDPARTQWRATFHFLRQQMVKEDGSPNKCLADFIAPPTASSPSGGPD
                     HLGAFAVTAGHGLNALVERFRAAHDDYSAILAEALADRLAEAFAEALHARVRREWQYG
                     RDEELTPEQLIAEAYRGIRPAAGYPACPDHTEKQTLWDVLRAESLTGIRLTESFAMWP
                     GSSVSGLYFAHPEAKYFGVGKLERDQVVDLARRKRMELQECERWLRPYLNYEAA"
     gene            complement(11935..12855)
                     /locus_tag="PKK20_01920"
     CDS             complement(11935..12855)
                     /locus_tag="PKK20_01920"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNU98678.1"
                     /translation="MNLSVQIGRLTLRNPVTVASGTFGYGVEYARLLDLNRLGALAVK
                     GIRLDPVRGNPTPRTAEVASGMLNAIGLQGPGVDGFIERYWPFLAGLEVPTIINIWGT
                     TVEEYAEVARRFDALGGVGALELNVSCPNIKEGGAQFGTDPRLLHQVVAACRKATSLP
                     LITKLSPNVVNIAPYAKAAEEAGCDALSITNSFPAMAIDIETRRPRLANGTGGLSGPC
                     IKPIAIKLVWDAAKAVRIPIIGMGGIQSAADAIEFMMAGATAVAVGTANFYEPQTALQ
                     VIDGLGDFMRRHGIEEVREIVGSVRIEKRS"
     gene            complement(12861..13646)
                     /locus_tag="PKK20_01925"
     CDS             complement(12861..13646)
                     /locus_tag="PKK20_01925"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNU98679.1"
                     /translation="MIQNTVRILRNDREADLYYRLTLRAPRVAPLIQPGQFAHLRIGF
                     LGEALLRRPFSVFQVADETFSILYKTVGKGTEALARMRPGDDLSVIAPLGHGFTVPQP
                     GGETPLLVAGSYGMAALYLLAQRSPQKGVVFVGGRRSADILCVEEFRALGWDVRVTTE
                     DGSRGEHGLVTRPLIDEVRRQAGGRKLFACGPTPMLEAVGRIASEFGVPAELSMDEPM
                     CCGVGVCLACVIPIRKGDRWEYQRTCTEGPVFDARTIAWDVRG"
     gene            complement(13643..14908)
                     /locus_tag="PKK20_01930"
     CDS             complement(13643..14908)
                     /locus_tag="PKK20_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNU98680.1"
                     /translation="MQIEIINTGTELMRGDVLNTDHQWLCRQLADAGYTVARQVAVDD
                     TAHHIQEAAREALGRADIVITSGGLGPTSDDSTRDAIAALLGRRLIEDASVRAWIDAW
                     FAARNRPQPPATRIQAMVPEGAIVIPNTHGTAPGLRMRCGHGAMADSTVAADKWLIML
                     PGPPRELQPMFLEGVLPWLKASFPPPGPAVCRILRSTGLGESVIQEMVGEPLCRLGGG
                     GLEVGYCARPGEVDVRLTARGAAAEALVADAEAVVRGRLGDHVFGVGGELLEQVVVRL
                     LTDSRRTIATAESCTGGRLSNRITDVPGASAVFVGGAVTYSNDAKQRMLGVRSETLAA
                     HGAVSEATAREMAEGARARLGADYALATTGIAGPSGGTETKPVGTVFIAMAGPDRTVV
                     RHEWNRFDRETFKRVTTQQALDLVRRELL"
BASE COUNT         2823 a         5374 c         4517 g         2490 t
ORIGIN      
        1 gtagtacatg tagatctctc atgaatcgtt gggccgctct tggtcaggcc tggctgggaa
       61 cagcaccggg ttcggctgag gactcggcag acgccgccac aagacgctgc tcgagaaacc
      121 ggttggccat gatgagcacc ccggcctgga gggaaaacgt cttcatggcg tattcgctgt
      181 cgccgtgcag gaagaggaaa ctgatggcgc ttcccatcca gatgccggac accaccgagc
      241 aggcgcggga aaaattgtcc tcgcagccat ggcggcgaag atgctggatg atgcgccagg
      301 ccacccgcgt cccgccgtac atgaacagca acatccccgc ggtgccaaac acaccagtgt
      361 tcaccatcag accgacaaag ccgttgtaga acacaccctg gttgatgtgc atcatcgtgc
      421 cggtcggatc gaaggcccgc gaatactcgt cgagataccg cgcgaagccg cgtcccaccc
      481 acaaatactg cgggatcatc tcgaggccga cacggaacat ggtgcgccgc gccgtcattg
      541 tgccgatggc gtccacccgc gccgcattgt cgatggaaat gccgggcagg aacgccacgg
      601 cgcgctgggc cgccagcggc atgcggtccg cgaacgcata gagcaccgca aacgacggga
      661 tcaggatcag cagggcgatc aactgattgc gcacggtgaa gaaccgctgg gcgaagacgc
      721 agaagagcat gacgaacccg gtcgtgacca aaaccacgcg gtggccgctg agcatgccca
      781 ccagcagcac caggacagtc accggcagca gccaaaccgc cctgcgcccg acaaaatcct
      841 tggtgttgtt gtagaccaga acgacgaaga aaaggcccat gctgaccagc gcgaatgact
      901 ggaaacgccg aatcccgaat cgcatcgatt gaaactcgaa actggcggcg tccacgggca
      961 attcgacaaa gtacagggcg ttgaccagcc ccccgccgaa ggtgaaggca aaatccgcaa
     1021 tcaaatacgt gccggtgaga agccattgga ttgtcaacaa tttgaccagc aaccgctcat
     1081 tgctctgcag catcacaaag agcagcggga agatcgcgca cagcagttgc tgcagataaa
     1141 gccggcctcc cgcctgtccc gatcccagaa tccgcaggcc gaagccgcgc accatcatcg
     1201 tcaacaccaa cacgacgcaa tagatgattg aaccgatgaa gatccaacga tgcgccctga
     1261 gagtcgcccc cagatcgggc gaaccccgcc gcaccatgtg aagcacaatc acaccggtcc
     1321 aggccagcag cgccgcccct tcccacatgt acgggcgccc cggaatgaac ggcaggatga
     1381 gcgccgagct gaatgtcgtc acggtcacga gcatggccat gtgcgcgtga tacggcagcg
     1441 tcgccagcca caggattccc cccaccacaa aggccaccat gaactggtcc gacgccagaa
     1501 aaacgctcag actcagtgcg gccagcagga tgagcccgta ccacaaggcc ctgtgaaacc
     1561 gaatgcgata gtcaattgcg gataccatga tcttcgtcgt tcgaccgcag ccgcactgtc
     1621 gcgccccacc ccgacacgcc ctctgcccga aaggccgccg ccgcccaacc gcgacattct
     1681 gtcgccgacc catccgtgcg gagcaacaca tttattgcgc cgcccgaggc ctttgttgac
     1741 aatcccgccc gcctgtgggc ggacttctga cgcacactca ggcggcgcgc gcgcgcgcct
     1801 gcaggccaga cagcctcttg accggaatca ctatgccgac aactcgggat cgacagcggt
     1861 ggcgggtgct ctattgcgtc gacactctca gcgcaggcgg ggcggagcga cagacggtgg
     1921 aactcgtcac acgattgaat ctagaacgct tcgccccccg cctcatctgc ctgcacggca
     1981 aggcctacgg cgccagcctg cattttgcac cgcatctcga tcgcgcgaac gttccgtggc
     2041 gcgtcctcga cgtccgctgg tccccccctg gcttgctggc gggttgggcc cggctcatcg
     2101 ccgcggtctg gcgcatgcgc cccgcgcttc tccatgccat cagccaccac agcaatcata
     2161 tcgcccgact cggccgcgcc ctgatgcccc cccgcctgcg cctggtgacc gcggtgcgga
     2221 ccgaatacca gccgcgacag ctcttctacg agcggatcga gcaccgactc gcccagcgcg
     2281 tggtctgcaa cagcccgcac ctcgaacgcc aactccggga gcacagccgc atccccgccc
     2341 gcaagctggt ctgcatccca aacggcctcg attgcgagcg ctttggccgc aacccagatc
     2401 ccggtctgcg ggctcgcctc gccccaaacg cctcctgcgt tgtcgccatg ctgggccgca
     2461 tcacaaaaca gaagtcgccg cacctgctgg ccctggccct gggggaactc aaacgccgac
     2521 aggaattgcc aggcggcgtc cgggtgttcg ttgcgggcga gcacgaaagc gatgagatcc
     2581 agacccaact cgatgaggcg atccgccgcc acggcctggg aggcgtcatc acgcagttgc
     2641 cccaaaccaa ccagcccgaa gccctcctgc acgccgcgga cttcaccgtc ctggcatctc
     2701 tctgggaagg attgcccaac gcggttctcg aatcgctcgc ggccggacgc cctgcgctgg
     2761 tgtccgaagc tgccaacgcg tccggggtca tcgaggacgg ggtcacggga tgggtggcgc
     2821 gcactgacga tgtgccgcat ctggccaccc gtctgggcga ggtcttccgg ctgtcgcccg
     2881 agcggctcga aggcatgcgc ccggcctgcc gggatcgcgc cggcatgtac agcatgacgc
     2941 ggatggtcca gcgctacgaa gaactctacg aggaactctt gtccggtgcc agccccgccc
     3001 ccggaactcg agccgagccc cagcctcgac gcgccccgac gacaaaggcg aagagggtcg
     3061 accggtaagc gcgccagttg aagggccaaa cgcaaatcgc cttgatgccg aatcggacgc
     3121 cgttccagca ttggccgcgc cggcggctgg ttccgctgat ctgaacggcc agatcgctca
     3181 gatcgcgccg ggacgccaga cccaggcgac gatgcttctc gaacatccgg ccgatggcct
     3241 gaagatgctc catcgggcgg gtgctcgtca gcgatgcctc gtgctccccg tattcgtaga
     3301 tgatgtaggg ccagtgaccc acctccgcca catagctcag gcgcagcatg aaatcgtagt
     3361 cctccaccat gcgcagcgag gcgtcatact cccccgcctg acgcgccgcc gcggcacggt
     3421 agagaaaggc cgccccgaac aggctgttgc gccagaaagt gtcgcgatag accggcggat
     3481 tgaagaccac cccctgctcc ctgcgctgga tcatgtcgcc gtagaccaac cccacctcag
     3541 gatgcccgcg cagataggcg ctcaggcgct cgagcgcctc gggaaagtac cggttgtcgt
     3601 cgctggtcca ggtccaaaac tccccgcgag ccacccgaaa cccgttgttc aacgcgcgcg
     3661 gcaggcccag attctgctcg cgcatgtacc cgacgcgcgg atcctgctct cgcattccgg
     3721 ccgcatactc ccgagtctcg tccccggacc cgtcgtcgac tatcagcaac tcgaggtccc
     3781 gctctgtctg ccccagaatg gaatcgacac tccgcggcaa gagcccggcc cgattgtagg
     3841 tcggcaggac aatgctcaca agcggcggcc ccggcctgcg gggagccagc cactccgtca
     3901 cgctctcggg tcgcagtttc atactcgatt cttcacccaa ctcgatcccg cgcgatcccc
     3961 cgtggattca tgcccccgga ctcgcacccg ccccccccgg gcgggaccgt cccatccgaa
     4021 cccgcgcccg gccgcttgaa ttcgccatag tgacgccgcg cccacgccac ggagaaaacc
     4081 ggcttgccat ccgccatctc gagccacgga tgcaagtcgc gcggcacctc gatcttcgtc
     4141 acgagccaag cgaagcaatt ggactggcgc gggaatacct tggtgaacag gcgcaccagc
     4201 ggcaacaggg cgcggagggt gcgagacgcc caccgattct tcattgccgc aatgcgcccg
     4261 tgcgggacgc gcagggaata gaaataccgg attcgcggca ggccgcgcgg ctcccatgcc
     4321 cggatgactt tttcgacctc gcgctccgac cagcgataga cgaagttcgg gatcgccgtg
     4381 ttgtcgacgc cgcccgaggt catgcggttg cccgccaccg ccgccacctc gtactcttgg
     4441 ccgaaatcca ggcgcacacc caaccggacc aggaacgtgt cccgcggctc gaataccagg
     4501 atgccgcgcc gcgccacgcg gtacatctcg agcatggcgc gatgcggcga gtgacaatgg
     4561 tgcaacccct cgtgcgcaac cacgaaatcg aaggcgccat ccgggaatcc cagggcctcg
     4621 gcatcctgca agctccaggt gtaaggggcc accagatcct ccgtcacgcc cggctcgatg
     4681 tttgaaatcg tcacccgcgt gaaccccaca tgacgcagca catcgcgatc ctgatcgctt
     4741 ccgcaaacga ccagaagctc cgtgtcccgg cgcagcacac cctcgtcaat cagccgctgc
     4801 aaggtgcggg cgtaaaactc ttccatagcg tttaatgggg tcgcatctag atagttgaca
     4861 attcactcct cagtttcgcg acaaagcgct tgcgctccgc gtcctcgcca agcctccgcg
     4921 cgaaacgctt cacggcctgc gcccccgcct gatatttgat gcccacgcgg cgcaccgcct
     4981 ccgcaagccg cacccctcca tggcgaaccg ccacgtacat cactccgtcc cggccccaat
     5041 ccccgtgccg ctccgcccac cgctcccacg gctctccacg catcttctcc gccgccccca
     5101 cgagttcctc ccatccgacc cgccgccgca tccgtcgcgc cgctgtctgc tcctcctcat
     5161 tcaccgcgcg ccccgaaagc agcttccgcg cgtactcaat gtccccaagc accactccgg
     5221 cgaccatccc ctcccacggg ctctccaacc ccccttgccg aatcggttgc tccgtgtaag
     5281 ccttcaaggc cgcccggcgc tccgcccggc cccgccctcc gcaggcccgt tcgatcacgc
     5341 ccgtctccag ccaacccgca ttgggctcca gtcccatgta gaccctccag gaactccatt
     5401 gatactcgcg cagcgtctgg aggcggcgcc tgacaagttc ttctcccggg ttttcacacc
     5461 ccagcacccg ggcacggcgc tggtcgctcg tgttcagccc cagtcctgtg atccgcaccg
     5521 gattcaaatg cacatagcgt gccacctcga ccgccttgtc ctcctcctgg atcaacaccc
     5581 ccttgaatcg tccctgaaac accgttccac ggcaccgatg agcccaattg aacttgaccg
     5641 cataactcac gttgagccaa tggatggcct ggctgagatt ggcctccctg gtccgcacta
     5701 gcacatggta atgattgggc atcagcacga acgcgtgcac ctccagttca aaacgctccg
     5761 gcaactcggc cacaacaccc agaaaccgac gccgatccgc ttcatccagg aacaacgccc
     5821 ctccccggtg cccccgattc attacatgat accaggcgtc cgccacatca acccgcaacg
     5881 gccttgccat aggcccgaga cacaacaccg cgaatctccg tttgtcaact atatagatgc
     5941 gaccccattg ttcacccttc gaaagcggcg tcacattgga cgcggcgcgc catcgcactg
     6001 ttgcgtttcg gcgggccgtt gggcagcatc cctccggaca cgatctatgt gtggcatttg
     6061 tggcatctgg cattttgcct cgaacgagcc tgtggatgcg gagttgctgg cgcggatgac
     6121 gcgtcaattc gtgcaccgca gcccggacga ggaggggttc cactgcgaag gcggggtggg
     6181 cctgggcttc cgccggcttg gggacatcga cctggcgacc agccaccagc cgatgcccaa
     6241 cgaggacggc tcgctgcgcc ttgtggccaa cggtgagatc tacaattccc aggaactgcg
     6301 cgatcgtctg gccccgcggc acaccctccg gacccaaggc gacatcgaaa ccatcctgca
     6361 tgcctacgaa gagcacgggg tcgactgcgt ccatcagttt cgcgggatgt tcgctttcgc
     6421 catctgggac cgtgcccaac agcgcatgac cctggcagtc gaccggttcg gcaaaaagcc
     6481 gctctactat gccctggatg ggaagaagct ggtcttcggc tccgaactga agtgtctgcg
     6541 acaacacccg ggcattgcgc tcgatttgga ctacccggcc ctggacgagt acctgagcac
     6601 cggttacgtc cgcgcccccc gctcgatcta ccggaccatc cggaagctgc cgccgggaca
     6661 tacgctgacc gtcgcggcca acggcggctc gcgcctcgcc gagtactggc atccgcggct
     6721 ctgcgccccg ccggagcatt cacaggcctc gatcgaggaa ttggccgcgg agcttcgcga
     6781 gcgtctgctc gaagccgtcc ggctgcggat gatcagcgac gtgccgttgg gcgcgtttct
     6841 cagcggtggg atcgacagca gcatcgttgt cggtctgatg agccgcctca gttcccggcc
     6901 ggtgaagacg ttctcgatcg gattcgaggg ggacccgaac gacgagagcc cctacgcgcg
     6961 cgcagtcgcc gtccactgcc gaaccgacca cgtccacgag attgtcaaac ccgacgtcgt
     7021 cgacatcctg cccaagctgg tccgccacta cgacgaaccg ttcgccgaca actcgatgat
     7081 ccccacctat tatgtgtcgg tgatggcgcg taaggcggtg acgatcgccc tgagcggcga
     7141 cgggggggac gaggtatttg ccggctatca gtggtatcga cgcgcctatc gccacagccg
     7201 cctccaggcc tttatccccg gcctgctccg ccccgcggtg gccgtccttg cccgggtgtt
     7261 cccgcccgac agcacagtcc atcgctatct cggcgtcatc gatcaaccgc ccacctgctg
     7321 gggcctcgcc caggagtact tcaaccgcgg ccggcgggcc cggctgtaca acgactggac
     7381 gcggacgcag gtaggggaat cggacgcgga tgccgggcgg gaggcgattt tcgaatcggt
     7441 caaggggctg ggctacttgt cgcaacttca atacgccgat ctggtcggtt ttctgcccgg
     7501 cgacggcctg gtcaaggtcg accgggccag cacgctcgcg tccctcgaag tgcgcagtcc
     7561 gctcctcgac cacagggtgt tcgagttcat ggcgcgcgtg ccgccgcact acaaactcga
     7621 tggccgcgag agcaaggtcc tgctcaaacg cgccgccggc gaggtcctgc caccgtccat
     7681 cctcacccgg cgcaagcgcg gattcgatct gccggccggc gaatggctcc gcggtccgct
     7741 gcggcccatg ctcgaagacc tgctgctggc gcctggcgcc cgcacggcca cattcctcga
     7801 tcagaccggg gtccgtcgat tggcaaagga gcacgccgac ggccggatgc gccatgactc
     7861 gaggctctgg gcgctgctct gcctcgagtt gtggctgcgc gagttccctc agccaggagc
     7921 ggaaaccggc gccagccgac acacgtcgcc agagagccgc ggcaacccgc cgcgccccgc
     7981 agcgcccttg tgaacatccc cttccaaatc catgacagaa cactcaagcc accagaacag
     8041 ccgcgcagca gacttgcggc gcgtattgaa ggagcggatc gccatcatcg acggcgccat
     8101 gggcacgatg atccaggccc gccagctcga cgaagccggc tatcgcggcg aacgattccg
     8161 ggactggaca tgtcaggtca agggcaacaa tgacctgctc aatttgactc agccgcaact
     8221 cattcgcgag atacacacgg cctatctcga tgcgggggcc gacctgatcg agaccaacac
     8281 attcaactcg acgtcgatct cgctggcgga ctacgggctc gaggcgttcg cgcacgagct
     8341 gaatcttgtc ggggcccgcc tggcacgcgc ggcggcagac gcctacatgg ccgagcatcc
     8401 ggaacgcctc tgcctggtcc tgggcgccct gggacccacc agcaaaacag cctcactcag
     8461 tcccgacgtc cacaatcccg cctatcgggc tgtgacgttc cgtcaactga ccgacgcgta
     8521 tgcggaccag gcgcgcggcc tgctcgaagg cggagtcgac gccctgctgg tcgaaaccgt
     8581 cttcgacacc ttgaactcga aggcggccct gttcgccatc gccgagtgtt tcgagcagat
     8641 cggatgccgc gtcccggtca tggtttcgtt caccattacc gacaagagcg gacgcaccct
     8701 ctcaggacag acggtcgagg ggtactggaa ctcggtttcc catttcccgc tcctgagcat
     8761 cggcatcaac tgcgccctgg gcgcgaaaca gatgcgcccc tacatcgagg aactctcgag
     8821 gatcgccccg gtgtatctaa gctgctatcc caacgccgga ctgccaaacg cattcggcgg
     8881 attcgacgag accccggaac agatgagccg cgacttgcgc gacttcgccg tcaacggctg
     8941 gctcaacatc gtcggcggct gctgcggctc gacgcccgac cacatccggg ccatcgccgc
     9001 cgccgttcgt ggcgtgccgc cgcgtgtgcc gcccacggtc gagccgtggc tccgattgag
     9061 cggcctcgaa gccctctccc tgcgtcccga ctcgaacttc atgaacatcg gcgagcgggc
     9121 caacgtgacc gggtcgccca agttcgccaa gctgatcctc gcggggaagt tcgacgaagc
     9181 gctcacgatc gttcgccagc aggtcgagaa cggggcccag gtgatcgacg tcaatatgga
     9241 cgaagggatg ctcgattcgg agcaggccat ggtgcatttc ctgcacctga tcgccgcgga
     9301 accggacatc gcccgggtgc cgatcatgat cgactcatcc aaatggtcgg tgatcgaggc
     9361 cggcctgcaa tgcctccagg gcaaaggcat tgtcaattcc atcagcctca aggaagggga
     9421 ggacgaattc ctgagccacg cacgccggat tcgctggtac ggcgcggccg tggtggtcat
     9481 ggcatttgac gaacggggcc aggccgatcg ttacgagcgc aagatcgagg tctgcgcccg
     9541 cgcctaccgc ctgctcaccg agcgcgtcgg attcccgccg gaggacatca tcttcgaccc
     9601 caacatcctc accgtcgcga ccgggatcga ggagcacaac cgatacgccc tggacttcat
     9661 cgaggcgact aggtggatca agcagaacct cccgcgcgcc aaggtcagcg gcggcgtcag
     9721 caacatctcc ttcagcttcc gcggcaataa cctcgtccgc gaagcgatgc acagcgcgtt
     9781 cctctaccat gcgatccggg cgggactgga catgggcatt gtcaatccgg ggatgctcga
     9841 agtgtacgag gaaatcccga gcgatctgcg cgagcgggtg gaagacgtgc tgttcgaccg
     9901 tcggcccgac gccaccgagc gcctggtcgc tttggccgag tcggtcaagc aaaaggacaa
     9961 ggccgaggtg gtggacgacg cctggcggcg cggatcgatc gaggaacggc tccaccacgc
    10021 gctcgtcaag ggcatcgtcg acttcgtcga ggtcgacgtc gaggaggccc gccggaaata
    10081 cggacgtccg ttggacgtga tcgaaggccc gctgatggcc gggatgaaca tcgtgggcga
    10141 tctgttcggc gccggaaaga tgttcctgcc acaggtggtc aaatcggccc gcgtgatgaa
    10201 gaaggccgtg gcctacctca ccccctttct cgaagccgaa aagcaggccg gcgcgcgcgc
    10261 ccaaggcaag gtgttgatgg ccaccgtcaa gggcgacgtc cacgacatcg gcaagaacat
    10321 tgtcggcgtc gttctcggat gcaacaacta ccaggtcatc gacctcggcg ttatggttcc
    10381 gtgcgacacc attctcaaga ccgcgcgcga acagcgagtc gatgcgatcg gtctcagcgg
    10441 cctgatcaca ccctcgctcg acgagatggt gcatgtggcg cgcgaactcg aacgcgaagg
    10501 gttccgaatc ccgctgctga tcggtggcgc caccaccagc aaggcccata ccgcggtgaa
    10561 aatcgcgccc gcgttctccc agccggtcgt gcacgtgctg gacgcctccc gcgccgtgcc
    10621 ggtggtgagc cacctcctgc gtccggacca aaagggcgca ttcgtcgagc aagtccgtgc
    10681 cgaatacgag cggctgatcg ctcaacaccg cagcgcccgt ccgcggcttc tcaccatcga
    10741 ggaggcgcgc gcccgcgccc ctcggttgca atacgacgat atcccccagc ccgagttcat
    10801 cggaatccgc acgatcggca cccaaccccc ttctccaccc tccgatcgcc cggcttccac
    10861 aagccaggac gctcccccaa gagtggatca ccgcgccatc acgctcgccg atcttgtccc
    10921 ctacatcgac tggtctccct tcttccacgc ctgggaattg cggggacgct accccgccat
    10981 cctcgatcat ccccgacacg gcgaacaggc gcgccagttg ttcgaggacg gccagaaaat
    11041 gctcgaacgg atcgtttctg aacgcctgct cgaagcgcgc ggcgtatacg gcttcttccc
    11101 ggcggcgcgc gcgggcgacg acgtgcagat tttcgtcgac ccggcccgca cgcagtggcg
    11161 ggccacattc catttcctcc gccaacagat ggtcaaggag gacggatcgc ccaacaaatg
    11221 cctcgcggac ttcatcgcgc cccccacggc gtcgtcaccg tcaggcggcc cggatcacct
    11281 gggcgccttc gcggtgaccg ccggccatgg tctgaatgcg ttggtcgagc gattccgggc
    11341 cgctcacgac gattacagcg caattctggc cgaggcactg gccgatcggc tcgccgaagc
    11401 cttcgccgag gcgttgcatg cccgcgtccg acgcgaatgg cagtacggcc gcgacgaaga
    11461 actcaccccc gagcaactca tcgccgaggc ttaccgcggg attcgccccg ccgcgggtta
    11521 ccccgcatgc ccggaccaca cggagaagca aacgctctgg gacgtgctgc gcgccgagag
    11581 tctgacgggc atccgcctca cggagagctt cgccatgtgg cccggttcga gcgtcagcgg
    11641 cctttacttt gcccacccgg aagcgaagta cttcggcgtg ggcaagctcg aacgcgatca
    11701 ggtggtcgac ctggccagac gcaaacggat ggaactgcag gagtgcgagc gctggctgag
    11761 accgtacttg aattacgagg cggcttgacc ctcacagcag ggtcgtcaga cgttccccct
    11821 tgtcgagcga acctcgggtg acatcgagtt ggtgcggcaa cctgagttcg cgcacgaggg
    11881 ccgcccgctg atctccccag ccaagagccg gcggatgagc cggcagatgc tgattcagga
    11941 gcgtttctcg attctgacac tcccgacgat ctcgcgcacc tcctcgatcc cgtgacgccg
    12001 catgaagtcg ccaagaccgt cgatgacctg caaggcggtc tgcggttcgt agaaattcgc
    12061 ggtgcccacc gccaccgccg tcgcgccggc catcatgaac tcaatggcgt ccgcggcgct
    12121 ctggatgccg cccatgccga tgatggggat gcggacggcc ttggcggcgt cccagaccag
    12181 cttgattgcg atcggtttga tgcacggccc gctcagcccg cccgtgccat tggccagccg
    12241 gggccggcgt gtctcgatgt cgatggccat tgccggaaag ctgttggtga tggacaacgc
    12301 gtcgcaaccg gcctcctccg ccgctttggc atacggtgcg atgttgacca catttgggct
    12361 caacttggtg atgagcggca gggatgtcgc cttccgacag gccgccacca cttgatgaag
    12421 cagtcgcgga tcggttccga actgggcgcc gccttccttg atgttcggac acgagacgtt
    12481 cagttcgagc gcgccgacac cgccgagggc gtcgaatctc cgcgccactt cggcgtattc
    12541 ctcgacagtc gtcccccaga tattgatgat cgtcgggacc tcgagccctg cgaggaacgg
    12601 ccagtaccgc tcgatgaaac cgtcgacgcc gggcccctgc aagccgatgg cgttgagcat
    12661 accgctggcc acctcggccg tccgcggcgt cggattcccg cggacgggat ccaaccggat
    12721 gcccttcaca gccagggcgc cgagtcgatt caaatcgagc agtcgggcgt actcgactcc
    12781 gtagccgaac gtcccggaag cgaccgtcac cggattccgc agtgtcaacc ggccaatctg
    12841 gaccgacagg ttcatcgcgt tcagcctcgc acgtcccagg cgatcgtccg cgcgtcgaag
    12901 accggcccct cggtgcaggt gcgctggtat tcccatcggt cgccttttcg tatcggaatg
    12961 acacacgcca ggcacacccc aacgccgcaa cacatgggct cgtccatcga gagctccgcc
    13021 ggcactccaa actcggacgc gatcctgccg acggcttcga gcatcggcgt cggcccgcag
    13081 gcaaagagct ttcgtccgcc cgcttggcga cgcacttcgt cgatcaaagg tcgcgtcaca
    13141 aggccgtgct cgccgcggct gccgtcctcg gtcgtcaccc gcacatccca gcccagcgcc
    13201 ctgaactcct ccacacacag gatatccgcg ctccggcgtc cgccgacgaa cacgaccccc
    13261 ttttgcggcg agcgctgggc caacaggtag agggccgcca tgccgtagct ccccgcgacc
    13321 agcaggggcg tttccccacc cggctgcggc accgtgaatc cgtgaccaag tggcgcgatg
    13381 acactcaggt cgtctcccgg acgcatccgc gccagggctt cggtgccttt gccgaccgtc
    13441 ttgtatagga ttgagaaggt ctcatccgca acctggaaga cgctgaacgg acgccggagg
    13501 agcgcctcgc ccaggaaccc gatgcgcaag tgcgcaaact gccccggctg gatcagcggc
    13561 gcaacccgcg gcgcccgcaa tgtaagacga tagtacagat cggcctcgcg atcgttccgc
    13621 aggatgcgca ccgtattctg aatcatagta gttcgcgcct aaccaaatcg agggcctgtt
    13681 gcgtggtgac gcgcttgaat gtctcccggt cgaaccgatt ccactcgtgc cggacgacgg
    13741 tccggtcagg accggccatg gcaatgaaga ccgtgccgac aggtttggtc tcggtgccgc
    13801 ccgacggacc cgcgataccc gtcgtggcca gggcgtaatc ggccccaaga cgcgcccgcg
    13861 cgccctcggc catctcgcgg gccgtggcct cgctgacggc gccgtgggcc gccagtgtct
    13921 cggaccgcac acccagcatc cgctgtttgg cgtcgttgct gtaggtgacg gcgccgccca
    13981 cgaacaccgc cgatgcgccc ggcacgtcgg tgatgcggtt ggatagtcgc ccgccggtgc
    14041 acgactcggc cgttgctatc gtccgccgac tgtccgtcag aaggcggacg accacctgtt
    14101 cgagcagttc gcccccaacg ccgaacacgt ggtcaccgag ccgcccgcgc accacggcct
    14161 ccgcgtcagc caccagcgcc tcggccgccg cgccgcgcgc cgttaggcgc acatccactt
    14221 caccgggccg cgcgcagtac ccgacttcga gcccgccgcc gccgagcctg cagagcggct
    14281 ccccaaccat ctcctggatc accgactctc ccaaacccgt gctccgcagg atgcggcaca
    14341 ccgccggtcc cggcggcgga aaggacgcct tgagccacgg aagcactccc tcgaggaaca
    14401 tcggctggag ttcgcgcggc gggccgggca gcatgatcag ccacttgtcg gctgccaccg
    14461 tggaatccgc catcgcgccg tgaccgcatc gcatccgcag gcccggtgcc gtgccgtggg
    14521 tgttggggat cacaatcgcg ccctcgggga ccatcgcctg gatgcgcgtg gcgggcggtt
    14581 gcggacggtt ccgtgcggcg aaccaggcgt caatccaggc ccgcacggaa gcgtcctcga
    14641 tgagccgccg cccgagcagg gccgcgatcg catcccgcgt cgagtcgtcc gaggtcggcc
    14701 ccaagccgcc gctggtgatg acaatgtcgg cccgtcccag cgcttcgcgc gcggcctctt
    14761 gaatgtggtg ggctgtatcg tcgaccgcca cctggcgcgc cacggtgtag cccgcgtcgg
    14821 ccaattgccg gcacagccac tgatgatccg tgttcagtac gtcgccccgc atcagttccg
    14881 tgccggtatt gatgatctcg atctgcacgc cgtccgcagt gtcatgccat ctggaccggc
    14941 ttgcaagccc gcgacaatgc atctggagat tgtcggcggg aatcgagtat gcgccgcgtg
    15001 gagtctccct gccgcgcctg cctatgccgt gcccgacacg gcagacaggc cgttgcgctc
    15061 tgcggcgcgg gcagggcacg cggcctggtg cgctctgttc gcgcacacgc aggcgttgga
    15121 gtggggacgt cctcgtcccc gccgatgaaa taaagtgaca ggttcgtaat tattgacacg
    15181 ccttctgttt tggcgtacat gtgt
//
LOCUS       DAOQRO010000026        15188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2980_length_15188_cov_5.947796, whole genome shotgun sequence.
ACCESSION   DAOQRO010000026 DAOQRO010000000
VERSION     DAOQRO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15188
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2980_length_15188_cov_5.947796"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1085)
                     /gene="trpS"
                     /locus_tag="PKK20_01935"
     CDS             complement(108..1085)
                     /gene="trpS"
                     /locus_tag="PKK20_01935"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNU98681.1"
                     /translation="MRILSGIQPSGALHLGNYFGMMKPAIELQAQGEAFYFIADYHSM
                     TSLFDPAQRRRNTLDVALDFLACGLDPQRSVFFRQSDVPEVPALTWMLSTVTPMGLLE
                     RCHSYKDKKAKLAGDDLDRVNHGLFAYPVLMAADILIYDSNVVPVGRDQKQHVEVTRD
                     IAIKFNNLYGETFVIPEPRIRDEVAVVPGTDGQKMSKSYGNTIEIFGEEQALRKKVMS
                     IVMDSRTPQEPKPDAERNNAIQLLNLVAPGEVARDYLERLRAGGLGYGDLKKALFGHY
                     WDCFAAARRRRAELASNLDYVEDVLRTGAEKARAQAGVVLRRARQAAGI"
     gene            complement(1283..2155)
                     /locus_tag="PKK20_01940"
     CDS             complement(1283..2155)
                     /locus_tag="PKK20_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNU98682.1"
                     /translation="MNPSVKAPAGQELSEKEKAALLTLLGDEDAAVYRAVKRRILAAG
                     RDAVAWLQPHTLSRESLLRRRATEIVHHFQQQDADNRFLAFCLTHGEDLDLERGSWLL
                     AQTQYPSINPAAYQALLDTFAAELRDRIADQHTPVGILAGINQYLFGELGFHGNQENY
                     YDPDNSYLNRVVDRRTGNPISLCAVYWFLGRRLRLPIVGIGMPGHFLCRYQSSKAAFY
                     IDAFNQGKLLTRADCVKYLHASGHGFQESFLAPASPGRALLRMCSNLHQVYAHLGLRA
                     EASRLQRYVVALAK"
     gene            2350..3228
                     /gene="atpB"
                     /locus_tag="PKK20_01945"
     CDS             2350..3228
                     /gene="atpB"
                     /locus_tag="PKK20_01945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNU98683.1"
                     /translation="MIAGAALAGPWAAGLAAEAAEQSGMPQNAVPLFGEHGFLTNSML
                     VTWGVALAIILFARIATRRMQAVPEGAQNFWEWMVESLYEFLEGIIGSDLVKRTYWFF
                     ATIFVFILASNWIGLIPGVGTIGWKSAGAHHIDTPLLRGVNADLNMTFAMAMIFFVLW
                     FYWALRANGPGGFLLHLFGPKGETSGALKLVMVVIFALVGVIEVVTIAFRPVTLSFRL
                     FGNVFAGENMLEAMARLVQEPAWLGALTSVLVPIPFYFLEVLVGLVQALVFMLLTAVF
                     TLLICSHEEGHEGVHH"
     gene            3326..3544
                     /locus_tag="PKK20_01950"
     CDS             3326..3544
                     /locus_tag="PKK20_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNU98684.1"
                     /translation="MLMPLLAEMTGNIHISLACLGAALGVGMIGMKASEAVGRNPGAS
                     TKILVQAILSTAFAEGIVFFAIFLTRGQ"
     gene            3620..4135
                     /gene="atpF"
                     /locus_tag="PKK20_01955"
     CDS             3620..4135
                     /gene="atpF"
                     /locus_tag="PKK20_01955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNU98685.1"
                     /translation="MFASIISDTARQFGVDWPHFLAQCISFSIVLFLLHRFAYKPILQ
                     ILEERRQRIKEGLENAERIKAELAKTEAARQEALNQANQQANRLIEEARVAASKVLEQ
                     ETQKAIATANQIVAKAREAGEAELVRMKAELRKEIGRLVVETTAKVTGKVLTVEDHQR
                     LAEETKRQLAA"
     gene            4132..4524
                     /locus_tag="PKK20_01960"
     CDS             4132..4524
                     /locus_tag="PKK20_01960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNU98686.1"
                     /translation="MRTSKQARRDAKHLFRGCCVDGLLDEGRVREAVRRLLEEKPRGY
                     LAVLSHFQRLVKLDIERRTATVESAVPLPSDLREDLKASLERVGGRGLSIAFAENPSL
                     IGGLRIKVGCDVYDGSIRTRLARLAESF"
     gene            4544..6076
                     /gene="atpA"
                     /locus_tag="PKK20_01965"
     CDS             4544..6076
                     /gene="atpA"
                     /locus_tag="PKK20_01965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNU98687.1"
                     /translation="MSTLLQEIEAQIAGVKAGVARENVGVVREIGDGVARIEGLTDAM
                     LNEMLDFGNGVVGLALNLEETEVGAIILGDYTSIKEGTEVRTTGRLLEVPVGRAMLGR
                     VVDALGRPLDGRGPIRAEAFYPVEKIAPGIIKRRSVSQPVQTGIMAIDAMIPIGRGQR
                     ELIIGDRSTGKTTIGIDTIINNARLNKLAEAAGDKEHRPLYSIYVAIGQKQSNVAQVI
                     NVLEKHEAMPYTIVVVAAASDSASNQYLAPFAGCAMGEWFMDNGMDALIIYDDLSKHA
                     VAYRQVSLVLKRPSGREAYPGDVFYLHSRLLERSARVSEQHGNGSLTGLPIIETQAGD
                     VSAYIPTNVISITDGQIFLETDLFYQGVRPAISVGISVSRVGSAAQVKAMKQVAGRVK
                     LELAQFRELAAFAQFGSDLDAKTQAQLERGKRIVEVFKQPQYSPIPVEVQVAVLWAVQ
                     NGLMDDVPVDKIKDFQGKLTDYLTSRKADLLAKITREKALSDPLSAEIKAAITEFKQT
                     YS"
     gene            6092..6970
                     /gene="atpG"
                     /locus_tag="PKK20_01970"
     CDS             6092..6970
                     /gene="atpG"
                     /locus_tag="PKK20_01970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNU98688.1"
                     /translation="MPSTRDIRRRIRSVKSTAQITKAMQMVASSKMRKAQEAAQTGRP
                     YARLMVKVLGEVTRHAGEFTHPLMEKRPVAKRAVIIVSTDKGLCGALNGNLLREAARY
                     DRQTTAFVVAGRKAAQFVARTRRQLAAEFTYRDTPSLAEARAISRFVRDLFLKKEVDA
                     VEVVFTRFISTLNQKPESFTFLPIGEIKGLQTDSSGRGAEDDLAKGATEFLFEPNADS
                     VLGALLPHYLSLVLFQVLLEAKASEHSARMVAMKNATDNANQLVRELTLEYNKLRQAN
                     ITKELLEISTAAMALE"
     gene            7399..8952
                     /locus_tag="PKK20_01975"
     CDS             7399..8952
                     /locus_tag="PKK20_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNU98689.1"
                     /translation="MSGGRDHIRAKLGTVPHKPGVYLMKDRFGTVIYVGKARDLRRRV
                     SQYFQPSRRMAWDLKFNALVAAIHDFDTHVVKSEPEALLLEGKLIKEFRPRYNISFRD
                     DKRFLLVKVNLADPIPRFTLTRIQHDDGARYYGPFSSSGPVRRTLALVRHRFNLRGCR
                     PLTPNEADYKHCLYAHLKVCTAPCIGNVTREQYRLQVQAACDFLDGHCAEMASEIEAE
                     MKQAAAALEFEKAAQLRDLLTDLRRTTERTRRFVRIPYSLPVAIDPAKDLEELASLLG
                     LPAPPERIEGFDISNISGTFAVASMVSFRHGRPDRAQYRRFRMRTVTGQDDFACMAET
                     IRRRYSRLLRERTGSAEQARPRLPDLILIDGGKGQLSVACAELARLGLDALPVIGLAK
                     EFEEIYRPGAREPLRWSPECGALKLLQRVRDESHRFANTYNAQLRLKKISESLLDEFP
                     GIGEQRKAALLKRFGSVQRLRRATVEALASVPGFGGKAAAELKAFLDARSAPSASPAP
                     SPPDAASTQ"
     gene            complement(9365..12028)
                     /locus_tag="PKK20_01980"
     CDS             complement(9365..12028)
                     /locus_tag="PKK20_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98690.1"
                     /translation="MPQSCRSLDMMTPEEHAALDRHAADAIVRLGPLSAFREMIAEND
                     ALIRGPQLDNGRLITVSRTAAYSALMANWAAERHRMLGYDKPFAVVALGGTGRQEVTP
                     YSDTDFAFLFDDAIEGNSFLLELQRELFHTRSFRDRCGFACEVLPFNPDDALRLSGKQ
                     LNAFLDLRPVYDPDGLTATFRERIRATFDPFEHFLHVRGIWKERLETAALAWDRVDRF
                     DIKNDALRVFLAGIWTLAGRGFLHSHDVYRALEDPRDLAAYDFLLRVRCFVHSRKPAP
                     RRRAPGGDHPEDVLRFDDFNAFGELLGPAADEHARIAFADEVRARLISARRRVARFTR
                     SVIERELRQGRPAGRGSPIVYGVGGLSHQGSSACRTSRDKSRAALSLLLASQRYGVPI
                     DASELGTTFRDIGDWLVPVPEVSALFYEPRGSLAESFAFLSQFDGAEDRLFPGYAKFE
                     ASLDARVMAEGKLLRGAIERRKIRTLEECAQRGRDLLARAISTERGAGALDGVSIDVE
                     AALLDPDRLAAVKLTLKTKRLPLTDADLEARADTARPLHERLSSGMSGIPLDDYYRVY
                     RTDCDFTAETVRTTEFLIRHWRALRERADADINDERQVAEFAALCENEDLLRSLFVFT
                     FADRAEWQSEQDDPTSWFNMRELYAKCMAHYRPGLDAKRSIETAGFTPDQAKVLKDFG
                     PDFYAGVYRRHANRFATHLLHLAEYPSTQDAKVSILRDGLSIILGVAARDVRGLAACI
                     TGALWRQKVALRQAHLFSAANHGLALDFFHVEPGEKTVGPHVATAIEHAIRQRLFIGD
                     RDEAGIPCVHGTTTLQEWRPGQYGLRFETGENADGAVYMLTYKVFRYLEGNIFGLAAH
                     AVRGRIYVSVYLSLPPHLTLDAARAIVTRHF"
     gene            complement(12103..13245)
                     /locus_tag="PKK20_01985"
     CDS             complement(12103..13245)
                     /locus_tag="PKK20_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU98691.1"
                     /translation="MAQVVFEGVSKTFPRPSGEAVRAVKDLCLTIHDGEIVVLAGPSG
                     CGKSTTLRLLAGLEEPTSGTIAIDGRVVNAIEPRDRDVAMVFQNCALYPHLTVYENLA
                     FPLRLRKRSRDEIERRVRATAATLELTRLLERMPDALSGGERQRVAVGRAMVRQPKVF
                     LYDEPLSNLDAGLRSRMRVELKRLHEHLGTTTLYVTHDQVEAITMGRRLAVMKDGAIQ
                     QVAEPLDVYRRPANLFVAEFTGSPPMNLFPGAIETGGSGVLFRPAPPPQPDRPAGAPL
                     PVAQAHRAALSAYAGKPVVLGLRPEAIVFEDGLACGPDGVRVSARAEAVERLGAETHL
                     HGSIGGIRFTARSGADRPLAPGQTVSVWLDLDRARYFDGESGRAID"
     gene            13404..15089
                     /locus_tag="PKK20_01990"
     CDS             13404..15089
                     /locus_tag="PKK20_01990"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNU98692.1"
                     /translation="MQIPFQLFPPFLASARAIAPVVLFLGLLLPASGELTAAGLEGLA
                     RPQSGRSMRATSTMRQGEVRRGGGERRLDPKADPKGDLEEASNYDNYRVPPGETHVLL
                     DAEGPGVITHIWITFLGPERQAWAQQGSANHQEMLLRMYWDGRERPAVEAPVGDFFAN
                     CFGLRREVISQPVVVEDADSYNCFWHMPFRKSARIEIVNESDKPISLLYYNIDWIKKD
                     ALPDDTPYFHAQYRQEYPVQKGQDYLLLETAGKGHYVGTVLAVRTRSPAWFGEGDEKI
                     YIDGEAKPSIWGTGTEDYFLSAWGLRTTSTPYFGVPYCDQWGIVGGKTSAYRWHVHDP
                     IVFNTGIKVTLEHFGWISPDENPDFKSTSWNEREDDYASVAFWYQTGEPTFAARAPHA
                     RERRLPSLERLTVYARDFADARHHGAGEATPQNLDLYEGAHLLYRATQPEGAWIEIPF
                     EIARKEPLRLLLNATKSYDFGRYQASLDGVKLGGPIDFYKAAVANEEIHLLDFWPEPG
                     RHTLRLECVGKNPVSQGYYLGLESVRLRERRPRVVDYGHDKDKDWRKERLLYQ"
BASE COUNT         2784 a         4679 c         5037 g         2688 t
ORIGIN      
        1 ttccttcatc cgggtgcctt tgccggccgc gagaatgaca gctttcatgg tcgggtgagg
       61 ctaacgggct gccgcatggg gatcaatggc gcagagatcg gccaggctca aatcccggcg
      121 gcctgtctgg cgcgtcgcag cacgacgccg gcctgggcgc gggccttctc ggcaccggtg
      181 cgcaggacgt cctcgacgta atcgagattg gacgcgagtt ccgcccgtct ccggcgcgcc
      241 gccgcgaagc agtcccagta atgtccgaag agggccttct tgagatcgcc gtatccgagc
      301 ccgccggcac gcaggcgctc gagatagtcc cgcgcgactt cgccgggcgc aaccaggttg
      361 agcaactgga tggcgttgtt gcgctccgcg tcgggtttcg gttcctgcgg ggtgcggctg
      421 tccatgacga tgctcatgac cttcttgcgc agggcctgtt cctccccgaa aatctcgatc
      481 gtgttgccgt agctcttgct catcttctgc ccgtccgtgc cgggaaccac ggcgacctcg
      541 tcccggatgc gcggctcggg gatgacgaag gtctctccgt agaggttgtt gaacttgatg
      601 gcgatgtctc gagtgacctc gacgtgctgc ttctggtcgc gtccgacagg cacgacattc
      661 gaatcgtaaa tgagaatgtc cgccgccatc agcacgggat aggcgaacag gccgtgattg
      721 acccggtcca ggtcgtcccc ggcgagcttg gctttcttgt ccttgtagct gtggcaccgc
      781 tcgagcaggc ccatcggcgt caccgtcgag agcatccagg tgagcgccgg cacctcgggc
      841 acgtccgatt gcctgaagaa gaccgatctc tgcggatcga ggccgcaggc cagaaaatcg
      901 agcgccacat cgagcgtgtt ccggcgccgt tgtgcgggat cgaacagcga ggtcatcgag
      961 tggtaatccg cgataaaata gaaggcctca ccctgggcct gcagctcgat ggccggcttc
     1021 atcatgccga agtaattgcc gaggtgcagc gcccctgaag gctgaatgcc agacagtatt
     1081 cgcatgcccc gacgctaaca gaggcgcatg ggccaacgca agaaggcact ggggtcgcat
     1141 ctaaatagtt gacaaatgca tgagcgccgt cttgtcatcc gggctttccg ggcagacgct
     1201 gggcggcgcc gcggatcgca agcggcgtgt tgcgatgccc ccgagcgatt tatcaactat
     1261 ttagatgcga ccccagtgcc tttcacttgg cgagggcgac gacgtagcgc tggaggcgag
     1321 aggcttccgc gcgcaagccg agatgcgcat agacctggtg gagattcgag cacattcgga
     1381 gcagggcgcg gcccgggctg gccggcgcca ggaacgactc ctggaacccg tggcccgagg
     1441 cgtgcaggta cttgacgcag tcggcacgcg tcaggagttt gccctgattg aaggcgtcga
     1501 tgtagaacgc cgccttcgag gactggtagc ggcagaggaa atggccgggc atgccgatgc
     1561 cgacgatcgg gaggcggagc cgtcggccca ggaaccaata gaccgcgcag aggctgatgg
     1621 ggtttccagt gcggcggtcc acgaccctgt taagatagct gttgtccggg tcgtagtagt
     1681 tctcctggtt gccgtggaag ccgagttcgc cgaagaggta ctggttgatg ccggcgagga
     1741 tgccgacggg ggtgtgttga tcggcgatcc gatcgcgcag ttcggcggcg aaggtatcga
     1801 gcaaggcctg ataggcggcc ggattgatcg acgggtactg agtctgggcg aggagccagg
     1861 aaccgcgttc gaggtcgagg tcctcaccgt gggtgaggca gaaggcgagg aatcggttgt
     1921 cggcgtcctg ctgctggaaa tgatggacga tctcggttgc gcggcgtcgc agcagggatt
     1981 cacggctcag ggtgtggggt tgcagccagg ctacggcgtc acggccggcg gcgaggattc
     2041 ggcgtttgac ggcgcggtag accgcggcgt cctcgtctcc cagaagcgtc aggagggctg
     2101 ccttctcctt ttcggagagt tcctggccgg cgggggcttt gacggacgga ttcatcgggt
     2161 gactcgagcc catagacagc acagcggggg gccgaaagca atcgggaaaa aaaccttgtg
     2221 ccttgggatg ggcttgggca acgtatccgc cgcttgcgtc cggactctgt ctggggcagg
     2281 aacgccgatt tcttccccgg gagcgccaaa gaaaaaaact tcgcgatgac gtgcggatgg
     2341 aaacacattt tgatagccgg cgcggctcta gcagggccgt gggcggcggg gttggcggct
     2401 gaggcggccg agcagtcggg catgccgcag aatgccgtgc ctcttttcgg cgagcacggt
     2461 tttctcacca actcgatgct cgtgacgtgg ggggtggcgc tcgccatcat tctctttgcc
     2521 cgcatcgcca cccggcgcat gcaggctgtg ccggaagggg cgcagaactt ctgggaatgg
     2581 atggtcgaaa gcctctacga attcctcgaa gggattatcg gatccgatct cgtcaagcgc
     2641 acctactggt ttttcgccac gattttcgtt ttcattctgg cttccaactg gattggtctg
     2701 atccccggtg tagggaccat cggctggaaa tccgcggggg ctcatcacat cgacacgccg
     2761 ttgctgcgcg gggtaaacgc ggatctgaac atgaccttcg cgatggcgat gattttcttc
     2821 gtcctttggt tttactgggc tttgcgcgcc aacggccctg gcggatttct cctgcacttg
     2881 tttgggccca agggcgagac gagcggggcg ctcaagctgg tcatggtcgt catcttcgcc
     2941 ctggtcggcg tcatcgaggt tgtcacgatc gcctttcgtc ccgtcacgct gagctttcga
     3001 ctcttcggca atgtctttgc gggcgagaac atgctcgaag ccatggcgcg gctggtgcag
     3061 gaaccggcct ggctcggggc gctgacgagc gtgctggtgc caatcccgtt ttattttctg
     3121 gaggtcttgg tgggtcttgt gcaggcgctg gtgttcatgt tgttgacggc ggtgtttacg
     3181 ctgttgatct gctcgcacga ggaggggcac gagggagtcc atcactgagc ctggggcgcc
     3241 ggggagtcga gatcccggcg agcgctttcg accgtcggac cgtgttcaat cgcgggggcg
     3301 gtcgaggaaa acgaaaggta cacgtatgtt gatgccactg ttggcggaaa tgacggggaa
     3361 catccatatc agcctcgcct gtttgggcgc ggcgctgggt gtggggatga ttggcatgaa
     3421 ggcctcggaa gctgtcggtc ggaatcccgg cgcatcgacg aagattctcg tgcaggcgat
     3481 tctgagcacg gcgtttgccg aagggattgt cttcttcgcg atcttcctca cccggggtca
     3541 atagccggtg tgggcctggg cgcacggctt cggccatgcg cccgggagcg cccgcatggt
     3601 catccggagt tcaattccca tgtttgccag catcatcagc gacaccgcac ggcagtttgg
     3661 ggtcgattgg ccgcacttcc tggcgcagtg catcagcttc tcgatcgtcc tgtttctcct
     3721 gcaccgcttt gcctacaagc cgatcctcca gattctcgag gagcggcgcc agaggatcaa
     3781 ggaaggcctc gagaacgcgg agaggatcaa ggcggaactg gcgaagaccg aggccgcccg
     3841 ccaagaggcc ctgaaccagg cgaatcaaca ggccaatcgc ctcatcgagg aggcgcgcgt
     3901 cgcggcgtcc aaagtgcttg aacaggagac ccaaaaggca atcgcaacag ccaaccagat
     3961 cgtggccaag gcgcgcgagg ccggcgaagc cgagctggtt cgaatgaagg cggaattgcg
     4021 caaggagatt ggccggctgg tggtcgagac cacggcgaaa gtgaccggca aagtgctgac
     4081 ggtcgaggat catcagcggc tggctgagga gaccaagcgc cagctcgcgg catgaggacc
     4141 tcgaagcaag cccggcggga cgcgaagcac ctcttccggg gatgctgtgt cgacggcttg
     4201 ctcgacgagg ggcgcgtgag agaagcggtt cggcgccttc tcgaggagaa gccgcgaggg
     4261 tacctggcgg tcttgagcca tttccaacgg ctggtgaagc tcgatatcga gcgccggacc
     4321 gcaacggtcg agagcgccgt gccgctgccg tccgatctgc gcgaggatct gaaagccagt
     4381 ctcgagcggg ttggagggcg cggtctgagc atcgcgtttg ccgagaatcc gtctctgatc
     4441 ggcgggctgc gcatcaaggt gggttgtgac gtgtacgacg gcagcattcg gacgcgcctg
     4501 gcgcggctgg cggagagttt ctgagttttt tcatcgaatt ggcatgagca ccttactgca
     4561 agagatcgag gcccagatcg ccggagtgaa agccggtgtc gcaagagaga acgtcggcgt
     4621 ggttcgcgag atcggggacg gcgtggcccg gatcgaaggt ttgaccgatg ccatgctcaa
     4681 cgagatgctc gacttcggca acggcgttgt tggtctggcc ctgaacctcg aggagaccga
     4741 ggttggcgcg atcatcctcg gcgactacac atcgatcaag gaaggcaccg aggtccggac
     4801 aacaggccgg ttgctcgagg tcccggtagg cagggctatg cttgggcggg tggtcgacgc
     4861 cctggggcgg cccctggacg ggcgcggccc gattcgggcg gaggcgttct accccgtcga
     4921 gaagatcgcg ccgggcatta tcaaacgccg gtcggtcagc cagccggtgc agacgggcat
     4981 catggcgatc gacgcgatga ttccgatcgg gcgcggccag cgggagctga tcattggcga
     5041 tcggtcgacc gggaagacga cgatcggcat cgacacaatc atcaacaacg cgcggctgaa
     5101 caagctggcg gaggcggcgg gcgacaagga gcaccgcccg ctctactcga tctacgtggc
     5161 catcggacag aagcaatcga atgtggccca ggtgatcaat gtgctagaga aacacgaggc
     5221 gatgccttac acgatcgtcg tggtggctgc agcctcggat tccgcgtcca accagtacct
     5281 cgccccgttc gccggttgcg ccatgggcga atggttcatg gacaacggca tggatgcgtt
     5341 gatcatctac gatgatttgt cgaagcatgc ggtagcctac cggcaggtct cgctggtgct
     5401 caagcgccca tccgggcgcg aggcctatcc gggcgatgtg ttttatctgc acagccggct
     5461 cctggaacgg tcggcgcgcg tcagcgagca gcatggcaac gggtcgctga ccgggcttcc
     5521 gatcatcgag acccaggcgg gcgacgtctc ggcgtacatc ccgaccaacg tgatctcgat
     5581 caccgacggc cagatcttcc tcgaaaccga cctgttttat cagggcgtgc gtccggccat
     5641 ctcggtcgga atctcggtct cgcgcgtcgg ttcggccgcc caggtgaagg ccatgaaaca
     5701 ggtcgcgggc cgggtgaagc tcgaactggc ccagttccga gagctggcgg cctttgccca
     5761 gttcggatcg gatctggatg ccaagacgca ggcccaactc gagcggggca aacggattgt
     5821 cgaggtgttc aagcagcccc agtacagccc gatcccggtc gaggtgcagg tggctgtcct
     5881 gtgggcggtg cagaatgggc tgatggacga tgtgccggtt gataagatca aggatttcca
     5941 aggcaagctg acggactatt tgacgagtcg gaaggcggat ttgctggcga agatcaccag
     6001 ggagaaggcg ctcagcgacc cgttgagcgc cgaaatcaag gctgccatca ccgagttcaa
     6061 acagacctac agctaaggga agcgaagccc catgccgagc acgcgggaca tacgccgccg
     6121 catccgatcg gtcaagagca cggcccagat cacgaaggcc atgcagatgg tggcctcgtc
     6181 caagatgcgc aaggcgcagg aggcggccca gacgggccgg ccatacgcgc ggctcatggt
     6241 caaggttttg ggggaagtca cgcgccatgc gggcgagttc acgcacccgc tgatggaaaa
     6301 gcgccccgtc gccaaacgcg ctgttatcat tgtgagcacg gacaagggcc tttgcggcgc
     6361 gctcaacggc aacctgctcc gcgaggcggc ccggtacgac cgccagacca ccgcgttcgt
     6421 cgtggcgggg cgcaaggcgg ctcagttcgt cgcccgaacc cggcggcagc tcgcggcgga
     6481 attcacctat cgggacaccc ccagtctcgc cgaggcgcgg gcgatttcgc gctttgtgcg
     6541 ggatctcttt ctcaagaagg aggtcgatgc agtcgaggtg gtgttcaccc gcttcatttc
     6601 cacgctcaat cagaagccgg agagcttcac attcctgccg attggcgaga tcaagggcct
     6661 ccagaccgat tcgtccggcc gcggcgcgga ggacgacctg gccaaaggcg cgacggagtt
     6721 cctcttcgag cccaacgcgg actcggttct cggcgccctg ctgccgcatt acctgagtct
     6781 tgtcctcttc caggtgttgc tcgaagccaa ggcgagcgag cacagcgcgc gcatggtggc
     6841 gatgaagaat gccaccgaca acgccaacca actcgtccgc gagctgactc tcgaatacaa
     6901 caagctgcgg caggccaaca ttaccaagga actgctcgag atctcgaccg ccgccatggc
     6961 gctcgagtag cggtcctctc cgccaccttg acgttcgggt tggggccgac atatgggcga
     7021 gcgtgaccag accgtctttg ccaccttttc ccagtgcgca ccgtgatcgc ccagctccgc
     7081 cgctgtcggt cgaacagccc ggtccgttcc agccactgcg cccaatctgg aaaatgaact
     7141 tcctccatgt tgtcggtttt gccggctcaa taatcgccgg ataaaccgac caagttgaag
     7201 tcacagaggt caaggctatg ctccttcgtc gcgtctcgcc agcgagccaa atcccctcca
     7261 gcaaaatcgg aagtaatttt tgcacagacc cttagattcg tgtcactcgt cgtccagttc
     7321 ctgtggggga gaggagaacc acgagtcgct tggcgattcc gttgcgcgcg ggccgcgtca
     7381 ggcagtatgg tgcgctctgt gagcggtgga cgggaccata tccgcgcgaa gctgggcaca
     7441 gtgcctcaca agccgggcgt gtacctgatg aaggaccggt tcggcacggt gatctatgtt
     7501 ggcaaggcgc gggatctgcg gcgtcgggtg agccagtact ttcagccctc gcggcgcatg
     7561 gcctgggatt tgaagttcaa cgccctggtc gcggccatcc atgacttcga cacgcatgtg
     7621 gtcaagagcg agccggaggc gctgctgctc gaaggcaagc tcatcaagga gttccgtccc
     7681 cgctacaaca tcagcttccg cgacgacaaa cgcttcctgc ttgtcaaagt caacctcgcc
     7741 gatccgatcc cgcgcttcac gctgacgcga atccagcacg acgacggagc gcgctactac
     7801 ggcccgttca gcagctcggg tccggtccgg cgcacgctgg cgctggtgcg gcacaggttc
     7861 aacctgcgcg ggtgccggcc tctaacgccc aacgaggccg actacaagca ttgtctatac
     7921 gcccacctca aggtctgcac cgcgccgtgc atcggcaacg tcacgcgcga acagtaccgt
     7981 ctccaggtcc aggccgcctg cgatttcctc gatggccatt gcgcggagat ggcgtccgag
     8041 atcgaggctg agatgaagca agccgccgcc gcgctcgagt tcgagaaggc ggcccagttg
     8101 cgcgatctgc tgaccgactt gcggcggacc acggagcgga cccgcaggtt cgtgcgcatt
     8161 ccctactcgc tgccggtcgc catcgatcct gccaaagacc tcgaagaact tgccagcctg
     8221 ctcggactcc ccgccccgcc cgagcgaatc gagggattcg acatctcgaa catcagcggc
     8281 acgtttgccg tcgcgtccat ggtcagcttc cgccacgggc ggcccgatcg cgcccaatac
     8341 cggcgctttc gcatgaggac ggtgactggc caggacgact tcgcctgcat ggccgagacg
     8401 atccggcgcc gctactcgag actgctccgt gagagaaccg gatcggcgga gcaggccaga
     8461 ccccggttgc cggatctcat tctgatcgac ggcggcaagg ggcagctcag tgtggcgtgc
     8521 gccgagctgg caagactggg cctggacgcc ctgcccgtga tcgggctggc gaaggagttc
     8581 gaggaaatct accgacccgg cgctcgggag ccgttgcgct ggagcccgga gtgcggcgct
     8641 ctcaagctgc tgcaacgggt gcgcgacgag tcgcatcgct ttgccaacac ctacaacgcc
     8701 caactccgtc tcaagaagat ttccgagagc ctgctcgacg agttccccgg catcggcgaa
     8761 cagcgcaagg cggccttgct caaacggttc ggatcggtcc agcgcctgcg gcgggccacg
     8821 gtcgaggcgc tggccagcgt gcccggtttc ggcggcaagg cggccgccga gttgaaggcc
     8881 ttcctcgatg cccggtccgc gccgtcggca tcgccggccc cctcgccgcc cgacgcggcc
     8941 tcgacacagt aacagaatgg gtcaaaccct ctggaagcct tgaccggtta gacatgttgc
     9001 cgacaagtca agtttctgtg ctgcggtaag ggtctgtgca aaaatcactt ccgattttgg
     9061 cggaaacgcc accgggccag atgcgaggcc cgagggagga agtgtatccc ctgagatact
     9121 cgaccgaccg aggaacgacg cagatggccc ggtgctgccc cgccccgaag ggccggaggg
     9181 ctttggctcg atggtcccgc ggccgcggga ttgctcctcg gtcacggacc gctgcgggta
     9241 tgctccctcg tcgcgcctgg ccagcgagcc aaatcccctc cagcaaaacc ggaattgatt
     9301 tttgcacaga ccctaagcgg atcaaggatt ccactccgcc ggattcattg cagcagtgcc
     9361 ctcctcagaa gtgcctggtc acgatcgcgc gcgccgcgtc gagtgtgagg tgagggggca
     9421 ggctcaaata aacggacacg tagatgcgtc cccgcacggc atgggccgcg aggccgaaga
     9481 tgttcccctc gaggtatcgg aaaaccttgt aggtcaacat atacacagcg ccgtcggcat
     9541 tctcacctgt ctcgaagcga agtccgtatt gacccgggcg ccattcctgg agggttgttg
     9601 ttccgtgaac acacgggatt ccggcctcgt cgcggtcgcc gataaagagg cgctgtcgga
     9661 tggcatgctc gatggcggtc gccacatgcg gcccgactgt tttctcgcca ggttcgacat
     9721 ggaagaagtc gagcgccagg ccgtggttgg cggcggagaa gaggtgggcc tggcgcagag
     9781 caaccttctg gcgccaaagg gcgccggtga tgcaggcggc caggccgcgc acgtcgcgcg
     9841 ccgccacgcc gaggatgatt gaaagaccgt cccgaagaat gctgaccttg gcgtcctggg
     9901 tggatgggta ttcggccagg tggagcaggt gtgtggcgaa gcgattggcg tggcgcctgt
     9961 agacgccggc gtagaagtcg ggcccgaagt ccttcaagac cttcgcttgg tcgggcgtga
    10021 acccggccgt ctcgatcgag cgcttggcat cgaggccggg gcgatagtgc gccatgcatt
    10081 tggcgtagag ttcgcgcatg ttgaaccagg aggtcggatc gtcctgttcg gactgccact
    10141 cggcgcggtc cgcgaaggtg aagacgaaga gggaccggag gaggtcttca ttttcacaca
    10201 gcgcggcgaa ctccgccacc tgccgctcat cattgatgtc ggcgtcggcc cgttccctga
    10261 gggcgcgcca atgccggatc agaaactcgg ttgtgcgcac cgtctcggcg gtgaagtcgc
    10321 agtcggtgcg gtagacgcgg taatagtcat ccagaggaat gcctgacatg ccggaggaga
    10381 gccgttcgtg caggggccgc gcggtgtcgg cccgcgcttc gaggtcggcg tcggtcaacg
    10441 gaagccgctt ggtcttcagc gtcagtttga ccgcggcgag gcgatccgga tcgaggagcg
    10501 cggcctcgac atcgatcgag acgccatcga gcgcgccggc gccgcgttcg gtcgaaatcg
    10561 cgcgcgccag caggtcgcgt ccgcgctgcg cgcattcttc gagagtccgg atcttccgcc
    10621 gctcgatcgc cccgcgcagc agcttgccct cggccatcac gcgggcgtcc agcgaggctt
    10681 cgaacttggc gtagccgggg aacagccggt cctcggcgcc gtcgaactgc gagagaaacg
    10741 caaacgactc ggcaagactc ccgcgcggct catagaagag ggccgacacc tccggcaccg
    10801 gcacgagcca gtctccgatg tcacggaacg tggttccgag ctcggaagca tcgatcggca
    10861 cgccgtaccg ctgcgaggca agcagcagcg agagggcggc ccggctcttg tcccgcgatg
    10921 tgcggcacgc ggaagagccc tgatgcgaca ggccgccaac gccgtacacg atcgggctgc
    10981 cccgcccggc gggccgccct tgccggagtt cgcgctcgat gacgctcctg gtgaaccgcg
    11041 ccacgcggcg ccgggcggag atgagccggg cccggacctc gtcggcgaac gcgatgcggg
    11101 cgtgttcgtc ggccgcgggt ccgagcagtt ccccgaacgc attgaaatcg tcgaatcgga
    11161 gcacatcctc cggatgatcg ccgccgggcg cgcgccgcct gggggccggc tttcgggaat
    11221 ggacgaagca ccgcacccgc aagaggaaat cataggcggc gagatcgcgc gggtcttcga
    11281 gcgcgcgata gacgtcgtgg ctgtggagaa agccgcgtcc ggccagggtc caaatgccgg
    11341 cgaggaacac ccgcagcgcg tcgttcttga tgtcgaagcg atccacccga tcccaggcca
    11401 gcgccgccgt ctcgagccgt tccttccaga tgccgcgcac atggaggaag tgctcgaacg
    11461 ggtcgaatgt ggcgcggatg cgttcgcgga acgtggctgt caggccgtcg ggatcgtaca
    11521 cgggccggag atcgaggaag gcgttgagct gtttgcccga gaggcgcagt gcgtcgtcgg
    11581 gattgaacgg caacacttcg cacgcgaatc cgcaccggtc ccggaaactc cgggtgtgaa
    11641 acagttcgcg ctgcaactcg agcagaaagg agttcccctc gatcgcgtca tcgaacagga
    11701 acgcaaagtc cgtgtccgag tacggagtca cctcctgccg gccggttccg ccaagggcaa
    11761 ccaccgcaaa cggtttgtcg tatcccagca tccgatgccg ctccgccgcc caattcgcca
    11821 tcagggcgct gtaagccgcc gttcgcgaca cggtgatgag ccgcccgttg tcgagctgcg
    11881 gcccccggat cagcgcgtcg ttctcggcga tcatctcgcg gaaggccgac aacgggccca
    11941 gccgcacgat ggcatccgcg gcgtgtcgat ccaaggcggc gtgttcttcc ggtgtcatca
    12001 tatcgagact gcggcatgac tgcggcatca ctgccgttcg acagaccacg gcatgctacg
    12061 ctcgccgtcc gacgcagcaa ccaagattct cccccctccc ggtcaatcga tggcccgtcc
    12121 gctttccccg tcaaaatagc gggcccgatc cagatcgagc caaacgctga cagtctgccc
    12181 gggcgccaac gggcggtccg cgcccgagcg cgccgtgaag cggataccgc cgatcgagcc
    12241 gtgcaggtgc gtctcggcgc ccaggcgttc gaccgcttcc gcgcgcgcgc tgacgcggac
    12301 gccatcgggt ccgcacgcca gtccgtcctc gaacacaatc gcctccggac gcagaccgag
    12361 cacgaccggc ttgcccgcat acgcggagag tgctgcccgg tgcgcttgag ccacgggcag
    12421 cggcgcgccc gccgggcggt ccggttgggg aggcggcgcg ggacggaaca ggacgccgct
    12481 cccgcctgtc tcgatcgcgc cggggaagag gttcatgggg ggtgatccgg tgaattcggc
    12541 gacgaagaga tttgccggac gacggtagac atcgagcggt tcggccacct gttgtatcgc
    12601 cccgtctttc atgaccgcca ggcgtcggcc catggtgatg gcctcgacct gatcgtgcgt
    12661 cacgtacaag gtggtcgtgc ccaggtgttc gtggagccgc ttgagttcca cgcgcatccg
    12721 cgagcgaagg ccggcgtcca ggttcgagag cggctcatcg tataggaaga ctttcggctg
    12781 acggaccatc gcccgtccca cagccaccct ctggcgctcg cccccggaca gagcgtcggg
    12841 catgcgctcg agcaatcggg tcaattcgag cgtcgcggct gtcgctcgca cccggcgctc
    12901 gatctcgtcg cgagagcgct tccggaggcg cagcgggaac gcaagattct cgtaaacggt
    12961 cagatgcgga tagagggcgc agttttgaaa gaccatggcg acgtcgcggt ccctgggttc
    13021 aatggcattc acgacgcgcc cgtcgatggc aattgtcccc gaagtcggtt cctcgagccc
    13081 ggccagcagg cgcagggttg tggacttgcc gcagcccgag gggccggcca ggacaacgat
    13141 ctcgccgtca tgaatcgtga ggcagagatc cttcacggcg cggacggcct ccccggaggg
    13201 cctggggaag gtcttgctca caccttcgaa gacaacctgc gccatgcgca tccgagtctc
    13261 cggaagacgc gcctcgatgt cgagtccgca ccgcggcaga gccgcaaccg aggcgacgca
    13321 agatgcgcgc gcgcgcgcct gtcgccgcgc tgtgctggtg tgttggcatt gccagcggga
    13381 tggcgatgtg gcaaacatcg tccatgcaaa tacccttcca gctcttcccg ccctttcttg
    13441 catcggctcg agccatcgcg cccgtcgtcc tctttctcgg tctgctcttg ccggcgtcag
    13501 gcgaattgac agccgccggg ctcgaaggtc tggccaggcc gcagagtgga cgctcgatgc
    13561 gtgcgacgtc gacgatgcgt cagggggaag tgcgccgggg cggcggcgaa cgcagactcg
    13621 atccgaaggc ggatcccaaa ggcgacctcg aggaagcgag caattacgac aactaccgcg
    13681 tccctcctgg ggagacacat gtgctcctcg acgccgaggg gccgggcgtg atcacgcaca
    13741 tatggatcac gttccttggt ccggagcgcc aggcttgggc gcagcaagga tcggcgaatc
    13801 atcaggagat gctgctgcgg atgtactggg acggacggga gcggcccgcg gtcgaggcgc
    13861 ctgtcgggga tttctttgcc aactgtttcg ggctgcgcag ggaggtcatc agccagcccg
    13921 tggtcgtcga ggatgccgat tcgtacaatt gtttctggca catgccgttc cggaaatcgg
    13981 cccggattga aatcgtcaac gaaagcgaca aaccgatcag cctactgtac tacaatatcg
    14041 actggatcaa aaaggatgcg ctcccggacg acaccccgta cttccacgcc cagtatcgcc
    14101 aggaatatcc cgtgcaaaag ggccaggatt acctcttgct cgaaaccgcc ggcaagggtc
    14161 actacgtcgg gaccgtgttg gcggtgcgga cccgaagccc agcctggttc ggcgagggcg
    14221 atgagaaaat ctacatcgac ggcgaggcca agccgtcgat ctggggcacc ggcacagagg
    14281 attatttcct gtcggcgtgg ggattgcgga ccacgagcac gccgtatttc ggcgtgccgt
    14341 actgcgatca gtggggcatc gtgggcggga agacaagcgc ctaccgctgg catgtccacg
    14401 acccgatcgt cttcaacacc gggatcaaag tcaccctcga acacttcgga tggatttccc
    14461 ccgacgagaa tcccgacttc aagtcgacaa gctggaacga gcgcgaggac gattacgcca
    14521 gcgtggcgtt ttggtatcaa accggcgaac ccacgtttgc cgcgcgcgcc ccgcacgcgc
    14581 gcgaacggcg cctgccgagc ctggaacgtc tcaccgttta cgcccgcgac ttcgccgacg
    14641 cccgtcatca cggcgccggc gaggcaaccc cgcagaatct tgacctgtac gagggcgcgc
    14701 atctgctgta tcgggccacg cagcccgagg gcgcctggat cgagattccg ttcgagatcg
    14761 ccaggaagga gccgctgcgc ctcctcctca atgccaccaa gtcctacgac ttcggacggt
    14821 accaggcttc actcgacggc gtgaagctcg gcggaccaat cgatttctac aaggccgccg
    14881 tcgccaatga agaaatccat ctgctcgatt tctggcccga gcccggccgt cacaccttgc
    14941 gcctcgaatg cgtcggcaag aacccggtct cacaaggcta ctacctcggg ctcgaatccg
    15001 tccggctccg cgagcgccgc ccgcgcgtcg tcgactatgg ccacgacaaa gacaaagact
    15061 ggcgcaagga gcggctcttg tatcagtgag gctgctttgc gttcacgcag gaccaatcca
    15121 tgaagtggac cgtggccccg tcgccgacac acgccgtctt cacttgacat ggcgggggga
    15181 cgagccgt
//
LOCUS       DAOQRO010000027        15155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_2988_length_15155_cov_4.384503, whole genome shotgun sequence.
ACCESSION   DAOQRO010000027 DAOQRO010000000
VERSION     DAOQRO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_15155_cov_4.384503"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKK20_01995"
     CDS             <1..238
                     /locus_tag="PKK20_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU98693.1"
                     /translation="GVTSGNVFQEFIAPAGATRLFLGIPDGFGFNGAPGAYDDNDGSY
                     MVRIGINQDPNIPEPVSMGVLALGLLGWTFFRRR"
     gene            801..2702
                     /locus_tag="PKK20_02000"
     CDS             801..2702
                     /locus_tag="PKK20_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98694.1"
                     /translation="MKTTTTHRALRVIVQMLAALLVAMPTRGGEPGLSRLCEIVFPAS
                     VLQRGDNEIAIASQSGSWALYDAVTLEAPADIALGKVEQGMRITEIEAPPVWVKAEGE
                     ARQPVSVSLRSEGELADVGFVFGGKEVGRAAVRAGMQRVDLTLPAVTAATSGRLEVRQ
                     GDRVVAARDVTLRPPRLRELWLLPHSHVDIGYTHVQDEVVGIQITNLLKGMELARASA
                     GNPDGMRFKWNPEAVWVLEHFLERATPAQRAEFVEAVRRGDVGVDGLYANMLTGLCRP
                     EELAQCLRYGARVADVTGVPVETAAICDVPGWTWGLTTMMGQAGVKYFMIGPNYSARI
                     GTIHQWANSPFYWRSPSGREKVLCYVVDNYHFLGDLESNVRQHSERLERASYAYDLAP
                     MLWVGTWPGGGVDNAPPDEQIVAKVQAWNDKHAAPRVIISLAGEFFREFERRHGAALE
                     EHAGDLTPYWEDGAGSTSLETAMNRASAERLVQAEMLQALLAPKERNAREVEAAWKNV
                     LLYSEHTWGAHNSISEPDAPFVTAQWKVKQAFALDADRQSRALLEAALRPHRAPADDA
                     EEKTIDVFNTTQWTRTDLVVLAAAASAGFDGVATERGRAVPAQRLGSGELASWRRMCP
                     RLARGGSTW"
     gene            2735..3796
                     /locus_tag="PKK20_02005"
     CDS             2735..3796
                     /locus_tag="PKK20_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNU98695.1"
                     /translation="MEGLTLRTDTLEVEMDGVTGALRSLRRKGVGGDFVDRRSPVGLN
                     DYRYVLGADAAGARVNGPVSWQVLDAGPLVATVRFESEAPGCRRLMREVRVIDGLDRI
                     ELVNHVDRLAVREKDSVHFGFGFDLPGGTVRMETPWAVVRPNVDQLPGACRNWFTVQR
                     WVDVSNSERGITLAALDAPLLQVGGITANLLGPVAREEWLPEAIESTTLYSWAQNNHW
                     FTNYKADQPGVTTFRYCLQARRGPYRAMASARFGIEASRPLIAVSVDPARAVPEPLLA
                     VDSPDVLVETVKVSEDGKGLVLRLFGVSGRTSRVRIDWQGMRPTAVYGTDLREQVLDR
                     CDGRVEVPGYGVAMVRAEF"
     gene            4362..4811
                     /locus_tag="PKK20_02010"
     CDS             4362..4811
                     /locus_tag="PKK20_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNU98696.1"
                     /translation="MNHNSIQQYYGDDHDRLDGLFREYQSGKAADPVGAIRAFEEFKA
                     GLERHIVWEEEILFPAFESKTGRVDGPTAVMRSEHRQIRGFLAAIAEKLARNDVRTEH
                     EEAGLLSVLGPHNQKEESILYPMIDRFSPAEERAEVFAAMAAYAGKT"
     gene            complement(4947..5249)
                     /locus_tag="PKK20_02015"
     CDS             complement(4947..5249)
                     /locus_tag="PKK20_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNU98697.1"
                     /translation="MSSFGPPRYSGARQTLRGVNFICNAPQAQAVSLIGDFNGWNPDA
                     NPMRQQPDGAWLIMVELRHGHHRYAFWVDGQLTLDPRAQGITRNDQGQRVSLVPVS"
     gene            complement(5284..6225)
                     /locus_tag="PKK20_02020"
     CDS             complement(5284..6225)
                     /locus_tag="PKK20_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98698.1"
                     /translation="MKHISTAATNKSDKAAREVPASLIHPVVIYPFRQPADYSDLTAL
                     YDLVARLNADRARYARPVTVLDRKTHFAMEGNQAFRDFRHQTVAKHSDVLDAWCVDTC
                     QMWYTGLGHAWEHGHSHDVYWLLPGDFNYGTPIGQQVLSRLNDLPEIIQELDQDFCVG
                     EITTDHSNPKQLIDTYGTFAMLYNWFPAEAREIRQMTERPRSEFFAIRHAFLGEVLRD
                     RWYAYEQTLVILLKAVLGKKKISRFSVGDLSDLPHGRESLAAAMQEVERTERVLRSFW
                     RERNQHNRTWPVEYRALATQSSDIRRTAFTLLENLLK"
     gene            complement(6341..7852)
                     /gene="araA"
                     /locus_tag="PKK20_02025"
     CDS             complement(6341..7852)
                     /gene="araA"
                     /locus_tag="PKK20_02025"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNU98699.1"
                     /translation="MIDLKQFELWFVTGSQSLYGPAVLREVAAHSQEIAAALSASAAV
                     PVRVVFKPVLTSPEAIAALCQEANTATACVGVVTWCHTFSPSKMWINGLRLLRKPVLH
                     LHTQHNRDIPWSSIDMDFMNLNQAAHGDREHGFIMSRLRMNRKVVVGHWQEPSVQQRL
                     GAWARAAAAWHDAQGARFARFGDNMREVAVTEGDKVAAQIRFGYGVNGYGVGDLVRCV
                     NGVTDADIDRLTREYEQTYAMASDLRAGGARHASVREAARIELGLRAFLTAGDFKGFT
                     DTFEDLHGLAQLPGIAVQRLMAEGYGFGAEGDWKTAALVRAMKVMASGLPGGTSFMED
                     YTYHFHPAGAKVLGAHMLEICPSIAAGQPSLQVHPLGIGGKADPVRLVFDTPAGPGLN
                     ASIVDLGNRFRLLVNEVDVVPAEVPMPKLPVARGVWVPRPNLETAAACWIHGGGAHHT
                     GFSRALTREHLEDYAEIAGIECVMIGADATVYRIKQELRWNEMYYALSQGWNG"
     gene            8168..10489
                     /locus_tag="PKK20_02030"
     CDS             8168..10489
                     /locus_tag="PKK20_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98700.1"
                     /translation="MKAPSRSIVCALALAVGLTCLSGCTREPSPGPPLTKVVINNAPD
                     LPPPVVAPQSFAAAPPAPVSPAPVSPATASTSAETAQADAPTAIVAPERIPLPPVVAE
                     VVDLAQAQVGDSVLLEYIAKSTTPFELSADQIIYLKDIGLSEQVLSALVARGDALRGA
                     ALTQALPADAAPVKPAAPTEVVPAPAAAQAAAPVYVENAPAPPPPPPQGGETVYSQPV
                     TQVTQNYFYTALAPYGGWVEIPSYGWCWRPTVAVVDPFWRPYCHGGRWLFTDGGWYWQ
                     SSYSWGWAPFHYGTWYMSPACGWVWVPGYTWAPAWVTWRYTDAYCGWAPLPPGCGWST
                     GIGLTFHGSRVSVGFSFGLGWDCFSFVHYRHFYDPHPYRHRLPRHEIASVFHNSRVIN
                     DYKHERNHGIVNHGIAPSRITSVTRTEIRKVQIRDVTGEPNAPRRADRLEHDGRELAV
                     YRPQPTAASAGGGAPVSRHTQDARQTATPQRVPAASSLSATTAPLRPPMISGGARTDS
                     PQPARPATAPATRPTQAAPAPATVGQANRTVTQPSRTSPAPVTRAVPSQPSRVERVPA
                     TPATRPSSGSGDVRPSAPTTRSAPSTATPPTRIPATRSAPLTTTPDRSPATRSAPSAA
                     TPSRGATPAAPVYAPRSSPPTAPVSRTPASSVAPSQSRPVQSAAPSSAQPRPSGQQGS
                     IYRTAPVTVSPSVGPPMSTRPAPAYNSSRGSSAVRSAAPSYGAPPQSAPSRSVTPPSS
                     APSRSSVNAPMQSAPRSGASPSGSGGRGRATRD"
     gene            10775..11719
                     /locus_tag="PKK20_02035"
     CDS             10775..11719
                     /locus_tag="PKK20_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98701.1"
                     /translation="MGVACGQAAERRVLYETGFEVSEGYARAQAGLALWEQGMGWLAE
                     GSGGCGLVDEFFPEYGQQAFIGFSAPAPKHDVLTVWRPLRVTAPPRGYSVLQFSVLMQ
                     VVDSDNHRYDDFQWSFYNTKDHRLFTLNFDNSRLEIFYALDDDSGFYSSGYLFDNDVI
                     YLLEVWMDFANNRWQAILNGVSIVDSQPITTTGARLDFSDADAVWVLGQKGKPGTNYL
                     LFDEYRITAEPASNTPPVLEIVGLHPAGQFELLLRGQPGRSYAIDVTDESFAEWLPLQ
                     TNAAPSGLWRFIDDSAIDYAVSYYRARQVSGGGVGSAP"
     gene            11827..12834
                     /locus_tag="PKK20_02040"
     CDS             11827..12834
                     /locus_tag="PKK20_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNU98702.1"
                     /translation="MENRPLGESGIEASVVGFGTWVLGGGTVWGNDTNDRESIYTLQA
                     AIDEGINLIDTAPAYGWGRSEKVVGEAIKGRRDRVIVATKCGLWTDDERGSFFTEMAG
                     RIMRRSLRPDTIQIEIDRSLRNLGVDCIDLYQTHWPACPPDKTPIAETMECLLKLKEQ
                     GKIRAIGVCNVTLDELKEHCACGPIASDQFRYSMLYRDPERDILPFCQANRIATLTYM
                     SLEQGLLTGKVGMDRVFKPDEFRSNADWNTWFLLPNRKRVLDLLAGWRDLADKYHASF
                     AQLVISWTAAQAGATHVLCGIRNVAQLRDNLGAARIKLDPADLARMRRDVVALGEPAR
                     L"
     gene            13390..14529
                     /gene="leuB"
                     /locus_tag="PKK20_02045"
     CDS             13390..14529
                     /gene="leuB"
                     /locus_tag="PKK20_02045"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNU98703.1"
                     /translation="MGKTTLKIAALAGDGIGPEIMREAIKVLRAVEKKHALKLDITEA
                     SVGWDAVDKEGKALPDSTLAVCKKSDAILLGAVGLHYRDAELPKEKRPERAAYLRLRR
                     EFGLFANFRPIRTHPALAFASPLRSERLGEGIDLLVVRESTGGLYHAYPKKTEEVLDA
                     RRQGAKPGKVVRAIDTMAITGSDVERIAHMAFRAAWLRRKKVTSIDKANILETSILWR
                     DVVTDVSRQYPDVVLEHMLVDTAAMQLVLRPTQFDVILGENTFAEILGDEAAALVGSL
                     GMLPSATLGATQQGRTFGLYEPAGGTAPDIAGKNLANPIALIRAAALMLRFGLGAPEI
                     AKAIESAIDKVITEGYRTKDIASAHDVKERQVGTSEMGDAIAAAI"
     gene            14753..>15155
                     /locus_tag="PKK20_02050"
     CDS             14753..>15155
                     /locus_tag="PKK20_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNU98704.1"
                     /translation="MAVEVSRIAWVDNLRTAIIVMVVNMHACVTYSHVGSWYIKEVPE
                     PAMGMKLAFIVWQFGLQSFFMGLLFFVAGFFSHRSLGRRGPARFLLERAFRLGLPALL
                     YMAIIHPFMVFVLLGHPRIPDRPPLGALCSAY"
BASE COUNT         2662 a         4608 c         5086 g         2799 t
ORIGIN      
        1 tggcgtcacc agcggaaatg tgtttcagga gttcattgcg cccgcaggag cgacgcgtct
       61 gtttctcggg attcccgatg gattcggctt caacggcgca cccggtgctt acgacgacaa
      121 cgatggttcg tacatggtgc ggatcgggat caaccaggat ccgaacattc ccgaaccggt
      181 gtcgatgggc gttctggcgc tcgggttgct gggatggacc ttttttcgcc gccgctagcg
      241 cgcatttctc accaacttct gaagaacgcg gagtcggtgt catcgaaaga tgcacccatg
      301 cgtgccggcc cggagcaggt tcagcgcgga gcgtgtgaac gatccggggt taatccggtc
      361 atttcacagg cttcggggtc tgatgcgaag cctcgaccga tttgcagccg tggatgcctg
      421 tgagcacgga catggttccg gacgccaggc tcagcgggtg ggagtgcccg tcacacccgg
      481 agtgcagcgt cagttcttcc gcgcactccg gcactgacca gtgttgaaga acttgtcggc
      541 tcattgacct cggtcagcgt ggagcccctc cggactctat cccaattgct gaaccaggtg
      601 ccagcccggt ccaggggtgg aggatttgtc ctggaaccga atgcgtggga ccgcttcgtt
      661 cgcgagtgat tccacagtct atcgccattg gtaagcgctt gcctggcgag ggcagcgacc
      721 ggataatcag ggcatgtcaa ccatggcgag tcgcttgtga cgatgagaga agcccagcga
      781 gcagcagcac gacgaaaacg atgaaaacga caacaacgca tagggccctt cgagtgattg
      841 tgcagatgct cgcagctctg ctcgtcgcga tgccgacgcg cgggggcgaa cccggtttat
      901 cgcgactttg tgagattgtc tttccggcgt cggttttaca gcggggcgac aacgaaatcg
      961 ccatcgcctc gcagtcgggg agttgggcgc tttatgatgc agtaacgctc gaggcgccgg
     1021 ccgacatcgc gcttggcaag gtcgagcaag gcatgcgaat caccgagatc gaggcgccgc
     1081 cagtctgggt gaaggcggaa ggcgaagcgc ggcagccggt gagtgtgagc ctgcggtcgg
     1141 agggcgaact tgcggatgta ggtttcgtgt tcggtggaaa ggaagtggga cgggcggctg
     1201 tgcgcgcagg gatgcagaga gtcgatttga cactgcccgc agtcacggca gctacaagcg
     1261 ggcggctcga agtgaggcag ggcgatcggg ttgtggcggc acgggatgtg accttgcgtc
     1321 cgccgcgatt gcgcgagttg tggttgctgc cgcattcgca tgtggacatt ggctacaccc
     1381 atgtccagga cgaggtggtc ggcatccaga tcacgaatct gctgaaaggg atggaactgg
     1441 cccgtgccag cgcgggaaat ccggacggga tgcggttcaa gtggaatccg gaggcggtgt
     1501 gggtgctgga gcactttctc gagcgcgcga cgccggcgca gcgggcggag ttcgtcgagg
     1561 cggtgcggcg tggggacgtg ggggtggacg gtctttacgc gaacatgctg acgggtttgt
     1621 gccgtccgga ggaactagcg cagtgccttc ggtacggggc gcgcgtggcg gacgtgacgg
     1681 gcgtgccggt cgagacggca gcgatctgcg acgtgccggg atggacgtgg gggttgacga
     1741 ccatgatggg gcaggcgggc gtcaagtact tcatgatcgg accgaactat tcggcgcgga
     1801 tcggcacgat acaccagtgg gccaacagtc cgttctattg gcggtcgcct tcggggcgcg
     1861 agaaggtgct ctgctacgtg gtcgacaact accatttcct gggcgatctc gagtcgaatg
     1921 tgcggcagca cagcgaacgg ctcgagcgcg ccagttatgc ctacgacctg gccccgatgt
     1981 tgtgggtggg gacctggccg ggcggcgggg tggacaatgc gccgcccgac gagcagatcg
     2041 tggcgaaggt gcaggcctgg aacgacaagc atgccgcgcc acgggtgatc atcagcctgg
     2101 cgggcgagtt cttccgcgag ttcgaacgcc ggcacggcgc ggcactcgaa gagcacgccg
     2161 gcgacctgac gccctactgg gaggatggcg ccgggtcaac ctcgctcgag acggcgatga
     2221 atcgtgcgtc ggcggagcgg ttggtccagg ccgagatgct gcaggccctg cttgcgccca
     2281 aggagcgcaa cgccagagaa gtcgaggcgg cgtggaaaaa tgtgctgctc tacagcgagc
     2341 acacctgggg cgcgcataac agcatctccg agccggatgc cccatttgtg accgctcaat
     2401 ggaaggtgaa acaggcgttt gcgctcgacg ctgaccgtca atcgcgcgcc ctgctcgagg
     2461 cggcgctgcg cccgcatcgc gcacccgcgg acgacgccga ggagaagacg atcgatgtct
     2521 tcaacaccac gcaatggacg cgcacggacc tggtggttct tgcggcggcg gccagtgcgg
     2581 ggtttgacgg tgtggcgacc gaacgcggcc gggccgtgcc ggctcagcga cttggttcgg
     2641 gcgagttggc ctcctggcgg aggatgtgcc cgcgtttggc gcgcggcggt tccacctggt
     2701 gaaacaagcc ggttcaacgc gcggaaaggc gcgcgtggaa ggcctgactt tgcgaaccga
     2761 cacgctcgaa gtcgagatgg acggtgtgac gggcgcgctc cgcagtctgc ggcggaaggg
     2821 ggtcgggggc gatttcgtgg ataggcggtc gcctgtcggg ttgaacgatt atcggtacgt
     2881 gctgggcgcc gacgcggcgg gtgcgcgcgt caacggcccc gtcagttggc aggtgctcga
     2941 cgccggcccg ctggtcgcca ccgtgcggtt cgaatcggag gcgcccggtt gtcgacgtct
     3001 gatgcgcgag gtccgggtga tcgacgggct ggatcggatc gagttggtca atcatgtcga
     3061 ccggctggcg gtgcgcgaga aggacagcgt ccatttcggg tttggattcg acctgccggg
     3121 cggcaccgtg cggatggaga ccccgtgggc cgtggtgcgt ccgaatgtgg atcagttgcc
     3181 gggggcgtgt cggaactggt tcacagtgca acgctgggtc gatgtatcaa acagcgaacg
     3241 cggaatcacg ctggccgcct tggacgcgcc attgttgcag gtgggtggga tcacggcgaa
     3301 cctgctcggt ccggtggcgc gcgaggaatg gcttcccgag gcgatcgagt cgacgacgct
     3361 ctattcctgg gcgcagaaca accattggtt tacgaactac aaggccgatc aaccgggcgt
     3421 caccacgttc cgctattgcc tgcaagcgcg ccgggggcca taccgggcga tggcgtcggc
     3481 ccgattcgga atcgaggcat cgcgcccgct gattgcggtg tctgtcgatc ccgcgcgcgc
     3541 cgttcccgag ccgctgcttg cagttgattc gccggacgtg ctggtcgaaa ccgtgaaagt
     3601 cagcgaggac ggcaaggggc tggtgctccg gctcttcggg gtgtcggggc ggacgagtcg
     3661 agtgcggatc gactggcagg ggatgcgtcc gacggcggtc tacggaaccg atctgcgaga
     3721 acaggtgttg gatcggtgcg atggcagagt cgaggtgccg ggctatgggg tggcgatggt
     3781 gcgcgcggag ttttagcgag gcgccgctga tcgagagccg gttgatgtcg ccgttgtccg
     3841 ggcgaaacag gtctttggtg gggtcagaaa ctgaactggc agactacgct gtctaatatt
     3901 acgcaggctc attcggccct gcacgagggc gtgccgatcg aggggcaggc cgcggacggc
     3961 gctcatggtt tcctgtttgg gctgatggcc gatccgggtg gagtggcgtg cggtgcacat
     4021 gaagacatcg gttttgcgac acgattgcgc ccagtgtggc gagacgatca gtatcggcca
     4081 tgagtttttt ctgcccacat ttcgacattg agaaagacta ttgcctggcc ctcgaggcgg
     4141 actgtgtgcc tggccggccg ggttgcgtat tgcggaacaa ctcggtcttc tatatccccg
     4201 ccgaggagcg tctcaaggaa cgcaaggcag cttcggtcgg tttggcgtct tcggacccag
     4261 cacaccacga agaggcgcgg tgcggtggtg gcgatctcga aggaggtgcg gccggggggc
     4321 ggggaaattc gcgccaagag cacagagcgc tggacactcc tatgaaccac aacagtatac
     4381 aacagtatta cggcgacgat catgatcgac tcgatggcct gtttcgcgag tatcagtccg
     4441 ggaaagcggc cgatcctgtc ggcgcgatcc gtgccttcga ggaattcaag gctggtctgg
     4501 aacggcacat cgtctgggag gaagaaatcc tgtttcctgc attcgagtcc aagacagggc
     4561 gtgtcgacgg cccgactgca gtgatgcgct ccgagcacag gcagattcgg gggtttctcg
     4621 cggccattgc ggagaagctg gcgcggaacg atgtccggac cgagcacgag gaggctggtc
     4681 tgctctccgt actcgggcct cacaatcaaa aggaggagag catcctctat ccgatgatcg
     4741 atcggttttc gcctgcggag gaacgcgccg aggtgttcgc cgcgatggcg gcgtatgcag
     4801 ggaagacata gcgctactgc cagcccgcac gttcgggctg ctcctgcgcg ccaattggct
     4861 tcatttgcgg ccggtgagat ggcgtcgatt ccccgcggag gcagggcgat agggaagccg
     4921 ggggcatgga ccgacgcggt ccgatctcaa ctcaccggca cgagggagac gcgttggccc
     4981 tggtcattgc gggtgatgcc ctgggcgcgc gggtcgaggg tcaactgtcc atcgacccag
     5041 aaggcgtagc gatgatggcc gtggcgcagt tcgaccatga tcagccaggc gccgtcgggt
     5101 tgttggcgca tcgggttggc gtccgggttc catccgttga aatcgccgat caggctgact
     5161 gcctgggcct ggggtgcatt gcagatgaaa ttgacaccgc gaagggtctg cctggcgccg
     5221 gaatatcgcg gcggcccaaa ggagctcatg tgtgtcattg gcaaagtagt tttgaaagag
     5281 cggtcacttc agcaggtttt cgaggagagt gaacgcggtt cgccggatgt ccgaggactg
     5341 ggtggcgagg gcccggtact cgacgggcca ggtgcggttg tgttgattgc gctcgcgcca
     5401 gaacgacctc aggacgcgct ccgtccgctc gacttcctgc attgcggcgg ccagcgactc
     5461 gcgtccgtgc ggcaggtcgg agaggtcgcc gaccgagaaa cggctgatct tttttttccc
     5521 caggacggcc ttaagcagga tgacgagcgt ctgttcgtag gcgtaccaac gatcccgcaa
     5581 gacctcgccc agaaacgcgt gccggatggc aaagaactcc gagcgcgggc gctcggtcat
     5641 ctggcggatt tcgcgcgcct cggccgggaa ccagttgtag agcatggcga aggtgccata
     5701 ggtgtcgatg agttgcttgg ggttgctgtg gtcggtggtg atttcgccga cgcagaaatc
     5761 ctggtcgagt tcctggatga tctcggggag atcattcagt cgggagagga cttgttgtcc
     5821 gatgggtgtg ccgtagttga aatcgccggg gagcagccaa tagacatcgt gggagtggcc
     5881 gtgttcccag gcgtgtccga ggccggtgta ccacatctgg caggtgtcca cgcaccaggc
     5941 atccagcacg tcggagtgtt tggcgacggt ctgatgcctg aagtcccgaa aggcctggtt
     6001 cccttccatg gcaaaatggg tcttccggtc gagaacggtc accgggcggg cataccgtgc
     6061 ccgatccgcg ttcaggcgcg cgaccagatc gtacaaggcg gtgagatccg agtaatcggc
     6121 cggctggcgg aaggggtaga tcacgacggg atggatcaga gaagccggca cttcacgagc
     6181 ggccttgtcg gatttgttcg ttgctgcggt ggatatgtgt ttcatgcgat tcactccgtg
     6241 tcttgtcgtg ccggcgcgac ctggcgctcc gacccgcgtc tgcgcgagcg aatggggggc
     6301 ggtgtcgcgt ggctgcacga cggttcgatg aggcagggtt tcatccgttc cagccttggc
     6361 tcaaggcata gtacatttcg ttccagcgaa gttcctgttt gatcctgtag accgtggcgt
     6421 cggcgccgat catcacgcac tcgattccgg cgatctcggc gtagtcttcg aggtgctcgc
     6481 gggtgagcgc tcggctgaag ccggtgtggt gggcgcctcc gccgtgaatc cagcatgcag
     6541 cggcggtctc gaggttaggt cgagggaccc agacgccgcg ggccacgggc agcttgggca
     6601 tcggcacctc cgcgggcact acgtcgactt cgttgacgag gagtcggaaa cggttgccca
     6661 gatcgacgat cgacgcattg aggcctgggc cggccggtgt gtcgaagacc aggcggaccg
     6721 ggtccgcctt gccgccgatg ccgagcgggt gcacttgcag cgagggttgg ccggcggcga
     6781 tcgacgggca gatctcgagc atgtgggcgc cgagaacctt tgccccagcc gggtgaaagt
     6841 ggtaggtgta gtcttccatg aacgatgtgc cgcccgggag acccgacgcc atcactttca
     6901 tggctcggac cagcgcggct gttttccagt cgccctcggc tccgaacccg tagccctcgg
     6961 ccatcaaccg ctgcacggcg atgcccggga gctgcgccag gccgtgcagg tcttcgaagg
     7021 tgtcggtgaa ccccttgaaa tcacccgccg tgagaaaggc gcgcagaccc agctcgatcc
     7081 gggccgcttc ccggaccgac gcgtgccggg cgccgccggc gcgcagatcg gacgccatcg
     7141 cataggtctg ctcgtattcc ctggtcaatc gatcgatgtc ggcgtcggtc acgccgttga
     7201 cgcagcggac gaggtcgccc accccgtacc cattgacgcc gtagccgaag cggatttgag
     7261 cggccacctt gtcgccttcg gtgacggcga cctcgcgcat gttgtcgccg aagcgggcga
     7321 agcgggcgcc ttgggcgtca tgccaagcgg cggcagcgcg cgcccatgcg ccgagtcgct
     7381 gttggacgga gggttcctgc cagtggccca cgaccacttt gcgattcatg cgcaggcggc
     7441 tcatgatgaa gccgtgctcg cggtcgccgt gggcggcctg gttgagattc atgaaatcca
     7501 tgtcgatgct tgaccatgga atgtcgcggt tgtgctgcgt gtggaggtgc agcaccggtt
     7561 tgcgcagcag cctgagccca ttgatccaca tcttcgacgg cgaaaaggtg tggcaccatg
     7621 tcaccacacc cacacaggcc gtggctgtat tggcctcctg acagagggca gcgatggctt
     7681 cggggctggt gaggaccggc ttgaaaacca cgcgcacggg cacggccgcg gaagcactga
     7741 gtgccgcggc gatctcttgc gagtgggcgg caacctcgcg caacacggcg ggaccgtaca
     7801 aggattgact accggtgaca aaccagagtt cgaattgctt gagatcgatc atggcgattg
     7861 aattcaggat tgagtgttga gtcgcactct agtctctcgc ctttgaaacg tcacggacag
     7921 aatggatgac ccgatgaata cgcgaggggc ggcggaggtc aattggcacg agtggagtga
     7981 gaagggcgtc cgagggcagg gaacgcaggg agtcgccatc ggaaacgcaa gccacccatt
     8041 cgcgtttgac catgaacgat agccatgcca tcgaaccggt gcgatccacc cgcctgagcg
     8101 cgggatggtc gggcgccgtc cggtgacacc ggccttctgg cagacaacca tcaacatgat
     8161 gatcgctatg aaagctccct cccgctcaat cgtctgcgca ctggctttgg cagtgggtct
     8221 tacctgtctc tctggctgca cacgggagcc atctcctggg ccgcctctga ccaaggttgt
     8281 catcaacaac gccccggatt tgccgccgcc ggtcgttgcg ccccagagct tcgcggcggc
     8341 gccgcccgcg ccggtttccc ccgcgccggt ctcccccgcg acggcctcga catcggccga
     8401 gacggctcag gccgatgcac cgacagcgat cgttgcgccg gaacgcattc cattgccgcc
     8461 ggttgtcgct gaagtggtcg atctggccca agcgcaagtt ggggattccg ttctgctcga
     8521 gtacatcgcc aagtcgacca ctcccttcga gttgagcgcg gatcagatca tttacctcaa
     8581 ggacatcggg ctctccgagc aggtgctgag cgccctggtg gcgcgcggcg acgcgttgcg
     8641 cggggctgcc ctgacccaag cccttccggc ggacgcggcc ccggtcaagc ctgcggctcc
     8701 gaccgaggtc gtgcctgcgc ccgcggcagc gcaagcggct gcgccagtct atgtcgagaa
     8761 tgcgcccgcg ccgcctccgc ctccgcctca gggcggggaa accgtctatt cgcagccggt
     8821 gacccaagtg acccagaact acttctacac cgcgctggcc ccatacggcg gctgggttga
     8881 gattccgagc tatgggtggt gctggcgccc gaccgttgcc gttgtcgatc cgttttggcg
     8941 tccgtattgc catggaggac gatggttgtt cacggacggc ggctggtatt ggcaatcgag
     9001 ttattcgtgg ggctgggctc cattccacta tggcacgtgg tacatgagcc cggcttgcgg
     9061 atgggtgtgg gtccccggct acacctgggc tcccgcctgg gtgacctggc gttacaccga
     9121 cgcctattgc ggatgggcgc ccttgccgcc cggctgcggt tggtcaaccg gcattggcct
     9181 gaccttccac ggatcccgcg tcagcgtggg attcagtttc gggctcggct gggattgctt
     9241 cagctttgtg cactaccggc acttctacga tccgcatccg taccgtcacc ggctgccgcg
     9301 ccatgagatc gccagcgtgt tccacaactc cagggtgatt aacgattaca agcacgaaag
     9361 gaaccacggg atcgtcaatc acggcatcgc tccgagccgg atcacctccg tcacccgcac
     9421 cgaaatccgc aaagtgcaaa tccgcgatgt caccggcgaa cccaacgccc cgcggcgcgc
     9481 ggaccgcctc gaacacgatg gtcgggaatt ggcggtctac cggccgcagc cgacagccgc
     9541 ttccgccggc ggaggggcgc cggtctcgcg ccacacgcag gacgcccgcc agaccgcgac
     9601 tccccaacgc gtccccgcgg caagcagcct gtcggcaaca accgcaccgc tgcgtccccc
     9661 gatgatctcc ggcggggcgc gaaccgacag tccacaaccc gcccgcccgg cgacagcgcc
     9721 agcgacgcgc ccaacgcagg ccgccccggc gcccgccact gtcgggcagg cgaatcgcac
     9781 cgtgacgcaa ccctcacgaa ccagccccgc accggtgacg agagcggtcc ccagccagcc
     9841 gagtcgcgtg gagcgtgtcc cggcgactcc cgccacccgg ccaagctccg gcagtggaga
     9901 tgtccgtccg tccgctccca caacccgatc tgcgccttcg accgcaacac caccaacgcg
     9961 catcccggca acccgatcgg cgccgttaac gacgacgccg gaccgcagcc cggcgacgcg
    10021 gtccgcgcct tcggcggcca cacccagtcg aggcgcgact ccggcggccc ctgtctacgc
    10081 gcctcggagc agtccgccga cggcccccgt gtcccgcacg ccggcgagca gcgttgcgcc
    10141 cagccaatcc cgccccgtcc aatcggcggc gccgagcagt gcgcagcccc ggccctccgg
    10201 acagcaaggg tccatctatc gcaccgctcc cgtcacagtg tctccttctg tggggccgcc
    10261 gatgtccaca cgacctgccc ccgcctacaa ctcatcccgt ggatcctcgg cggtccgctc
    10321 ggcggcgcct tcctacggcg caccgccgca gtccgctccg agccggtctg tgacgccgcc
    10381 gtcgagtgcg ccatcgcggt cgtctgtcaa cgcaccgatg cagtccgcgc ctcgaagcgg
    10441 cgcgagtccc agcggcagtg gcggccgggg gcgcgcgacg cgggactgac tttcgtgagg
    10501 acgcttgtca gccagcaatc ggcacaggat gaaaccggag tgtttcgctg gcatgttctc
    10561 cgccgcctgg cataaaactc gaacgccgtt ttgaaccgcc acgaatccca ttgcgcacat
    10621 gtttcccact gcacctgaga cagtcagacc tagcggcgca agccagcctg ccggcacgta
    10681 ttggcgggct gcgatgcgca gagtcggaaa agccgggttg tcggcggccg gtcgaggcct
    10741 cccaggcctg gttgcgtgtg tcttaatcct ggcgttgggt gttgcgtgcg ggcaggccgc
    10801 ggagcgacgc gtcctttacg agaccggctt cgaggtctcg gagggctacg cgcgggctca
    10861 ggctggcctg gcgctgtggg agcagggcat gggctggctg gccgagggct cgggcggctg
    10921 cggcttggtg gacgagttct ttcccgagta cggccagcag gccttcatcg gtttctcagc
    10981 gccggctccc aagcacgacg tcctcacggt gtggcgtccg cttcgagtga ccgccccgcc
    11041 tcggggttac tcagtgctcc agttttcggt tctcatgcag gtggtagact ccgacaatca
    11101 ccggtatgac gatttccaat ggagcttcta caacacgaaa gaccatcggc tgttcacgtt
    11161 gaatttcgac aactcgcgtc tcgagatttt ctacgcgctc gatgacgatt cgggattcta
    11221 ctcgtcggga tacctcttcg acaacgacgt catctacctg ctcgaggtct ggatggactt
    11281 cgccaacaat cggtggcagg ccatcctgaa cggcgtgtcg atcgtcgact cacagccgat
    11341 caccacgacc ggcgcgcgcc tggacttcag cgacgcggat gccgtgtggg tcctgggcca
    11401 gaagggcaaa cccggcacca actacctcct ctttgacgaa taccggatca ccgccgagcc
    11461 ggcatccaac accccgccgg ttctcgaaat cgtcgggctc catcccgcgg gccaattcga
    11521 gttgctcctt cgcgggcaac ccggccggag ctatgccatc gacgtaacgg atgagtcctt
    11581 tgccgagtgg ctcccgctgc agaccaacgc cgccccgagc ggcctttggc gcttcattga
    11641 cgattcggcc atagactatg cggtcagtta ctaccgggcc cgacaggtgt cgggtggggg
    11701 agttggttcc gcgccttgat cgagcggttg ttctgccttt ctgcctgcga atcgagaccc
    11761 ttgtcttgac tcgggaggca cggttgtcta aaggagaggg cgaaacgtct aatcagagcc
    11821 atcattatgg agaatcgtcc tctcggcgaa tcagggatcg aggcctcggt cgtcggattc
    11881 ggcacgtggg tcttgggcgg cggaacggtg tggggcaacg acaccaacga ccgcgaatca
    11941 atctatactc ttcaagcggc gatcgacgaa gggatcaatc tgatcgacac ggcccccgcc
    12001 tacggctggg ggcggagcga gaaggttgtg ggcgaagcca tcaagggtcg gcgcgatcga
    12061 gtgatcgtgg ccaccaaatg cggcttatgg accgacgacg agcgcggctc gttcttcacc
    12121 gagatggctg gacggatcat gcggcgcagc ctgcgtcccg atacgatcca gatcgagatc
    12181 gatcgcagcc tgcggaatct cggcgtggat tgcatcgatt tgtatcagac ccattggccg
    12241 gcgtgtcctc cggacaagac accgatcgcg gaaaccatgg agtgcctcct gaaactcaag
    12301 gagcagggaa aaatccgagc cattggcgtc tgcaatgtca cactcgatga actcaaggag
    12361 cactgcgcct gcggtccgat cgccagcgac cagttccgct acagcatgct ctaccgcgac
    12421 ccggaacggg acatcctccc cttctgtcag gccaacagga ttgccacctt gacctacatg
    12481 tcactcgaac agggtctgct gactggcaaa gtggggatgg accgcgtgtt caagcccgac
    12541 gagttccgga gcaacgccga ttggaacacg tggttcctgt tgcccaatcg caagcgcgtg
    12601 ctcgatctcc tcgccggttg gagggacctt gcggacaagt accacgcctc attcgcccaa
    12661 ttggtcatct cgtggacggc tgcccaggcg ggcgccacgc atgtgctctg cggcatccgc
    12721 aatgtggcgc aactccgcga caacctgggc gccgcccgga tcaaactcga tcccgcggat
    12781 cttgcgcgga tgcgccgcga tgtcgtggcc ctgggcgagc cggcgcggct ctaatgcgcg
    12841 tttctcacca acttctcgaa gacacggagc ctatgacgac caaccggtgc tcgatgcgca
    12901 ctgcgtcgag tgtcatcgac cggatggcgg caagatcgag gctgccaaac tcgatctgac
    12961 acccaacgcc ttgggtcgtt tgccgaacgc caggaacggc agttgatcga gttgcgcgag
    13021 gtcatggcgg gccttctgac cgcgcccggc gggcattgac acggatgctc cgcgaacctc
    13081 ggttcgttcg atccacaagc tgttggcggg aatctcggag ggccgagtcc cgcgaggcca
    13141 gtctccggca gtggcaacgg actcgtcacc gactggcacc tctttcactc gcgtcggcgg
    13201 gggacgaacc gtccccccac cagccttttc gcattcatga agttgcggca gggctggggc
    13261 cggacttgac ttcgccgggt gttggttgga cgctcacggg cacgcaatcg ccaaaacacc
    13321 gaagcgacct ggatcaatgg aaggctggct ggtgaggccg agttcaccga tttctctcaa
    13381 ggacaattta tgggcaaaac cacactgaaa atcgcggcgc tggccggcga cggcatcggc
    13441 ccggagatca tgcgggaagc gatcaaagtc ttgcgagccg tcgagaagaa acacgctctg
    13501 aagctggaca tcacggaggc ctcggtgggt tgggacgccg tcgacaagga gggcaaggcg
    13561 ctgccggact ccacgttggc ggtctgcaag aagtcggatg ccatcctgct gggggcagtc
    13621 gggctgcatt accgggacgc cgaactgccc aaggaaaaac gccccgagcg cgctgcctac
    13681 ctgcgcctgc gacgcgagtt cgggctgttt gccaatttcc gacccatccg aacgcacccc
    13741 gctctggcct ttgcctcccc gctccggtct gaacggctgg gcgagggcat tgatctcctg
    13801 gtggtgcggg agtcgaccgg cggtctctat cacgcgtatc ccaagaagac cgaagaggtc
    13861 ctggacgcgc ggcgccaagg ggcaaagccg gggaaggttg tgcgcgcgat cgacaccatg
    13921 gccatcacgg gttcggatgt cgaacggatc gcgcacatgg ccttccgggc agcctggctg
    13981 cgccggaaga aggtcacgag catcgacaag gcgaacattc tcgagaccag catcctctgg
    14041 cgtgacgttg tgaccgacgt gagccggcag tatccggacg tggtgctcga gcacatgctg
    14101 gtcgacacgg ccgcgatgca gctcgtcctg cgtcccaccc aattcgacgt catccttggg
    14161 gaaaacacgt tcgccgagat tctgggtgat gaggcggctg ccctggtggg gtcgttgggg
    14221 atgctgccca gcgccacact cggggcgaca cagcagggcc ggaccttcgg cttgtatgaa
    14281 ccggcgggcg gcaccgcgcc ggacattgcc ggaaagaatc tggccaaccc cattgctctg
    14341 attcgggccg cggccttgat gttgcgtttc ggcctcggtg cgcccgagat tgccaaggcc
    14401 atcgaaagcg cgatcgacaa ggtcatcacc gagggctatc gcacgaaaga catcgccagc
    14461 gcgcacgatg tcaaggaacg ccaggtgggg acgtcggaga tgggtgacgc catcgcggcg
    14521 gccatctgac cggtcggcgc gtccggctga ggtgcaaggc gaacagcgca gcgagcggtt
    14581 ccttttccgg caaggcgcgc tctaggccaa cccaatccgc atggccacga attgctccgg
    14641 tgttttctca cgcccttctc ctacccctcg ctcggagaat cttcgaacgt atcagaaagc
    14701 ggagtgaaac ctaaccgatc aaccacgaac ctttgaattc gaggattctc ttgtggctgt
    14761 cgaggtaagc cgcatcgcct gggtggataa cctgcgcacc gccatcattg ttatggtggt
    14821 gaacatgcac gcctgcgtca cgtacagcca cgttggcagt tggtatatca aggaggttcc
    14881 ggagcccgca atgggcatga agctggcctt catcgtctgg cagtttggcc tgcaatcgtt
    14941 cttcatgggc ctgctctttt tcgtggctgg gttcttctcg catcgatcgt tggggcgccg
    15001 cggacccgcc cgttttcttc tcgagcgggc tttccgcctg ggactcccgg ccctgctcta
    15061 catggcgatc atccacccgt tcatggtgtt tgtcctcctg gggcatccgc gcattcccga
    15121 tcgtccgcct ctcggtgcgc tctgttccgc ctacg
//
LOCUS       DAOQRO010000028        14732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3135_length_14732_cov_5.549295, whole genome shotgun sequence.
ACCESSION   DAOQRO010000028 DAOQRO010000000
VERSION     DAOQRO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14732
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_14732_cov_5.549295"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKK20_02055"
     CDS             <1..674
                     /locus_tag="PKK20_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98705.1"
                     /translation="PTNAIELTRLLEQRRVDPQSLHLRTVLLSRQTDTETRDLIAAGL
                     FAAVKCNFGVREVSDPGLCVECESGRFHVNEDHFLVEVQDGELVVTTLCREAMPMLRY
                     RTRVACEISREKCPCGRTGAVIHPGPRLDGRRLVNETPLYERQVAEVLAQTEAAGHPF
                     QIEVSDRHIVVRMDMCEDLFSDTIWVVERLTHVIESEFLARLGIEARVVFTSPRQKHK
                     PETVA"
     gene            complement(688..1059)
                     /locus_tag="PKK20_02060"
     CDS             complement(688..1059)
                     /locus_tag="PKK20_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98706.1"
                     /translation="MQPFQIIFNPTSAAELARMPKELQLEILGEFRGLPQEALHTDLE
                     RFGRLRRGGKTLHRFRLGDYRLYFERHELGLVVHRILSRNTLKDFQFRSNLPLGEDEA
                     LQDNPRFWELIENATAQVRRD"
     gene            complement(1077..1151)
                     /locus_tag="PKK20_02065"
     tRNA            complement(1077..1151)
                     /locus_tag="PKK20_02065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1116..1118),aa:Glu,seq:ctc)
     gene            complement(1269..1832)
                     /locus_tag="PKK20_02070"
     CDS             complement(1269..1832)
                     /locus_tag="PKK20_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU98707.1"
                     /translation="MKKTLLALASVLVAGAVMAADTSTGGTLQFKGYYSGANNMKVVV
                     NPVEEVSAAAGWTFGLFTETGAVVMGYKVGGAGALEPVTAGFTGVTGYANAGSTWKLD
                     GFAQGATVNFMVGAYKGSSYDVAGLWAGKWGTYQATLGGDDLTPPGPAGQLNYAGALT
                     KEIIIPEPSVLALGLLGMGAFLIRRRS"
     gene            complement(1898..2278)
                     /locus_tag="PKK20_02075"
     CDS             complement(1898..2278)
                     /locus_tag="PKK20_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98708.1"
                     /translation="MFGWDTPGQTLVPGEGVFVFLAGSADVTVTFVGDVNQGALSVPV
                     KAGFNMVSSPVPQAGGLVDPLAFPIQDGDTVYKHQRGGGYALPNSFSDMFGWDNGEPT
                     IEVGEAFWVLSTAAKTWTRTFTVQ"
     gene            3081..3800
                     /locus_tag="PKK20_02080"
     CDS             3081..3800
                     /locus_tag="PKK20_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNU98709.1"
                     /translation="MIGALLNAVGIVVGGLAGSWSARQLSPQRQRQLQVLLGAFTVFF
                     GLSIVWSGLRGSWTQFLGKFALMLAALMLGKLTGHLLRIQRCVNKAGRFAGNQLSPDQ
                     AQSNRSFSDVFAACAAVYCLAPLALLGSLAEGLHGDLRPLIIKMIMDGLAAMSFARML
                     PGAVLLSAIPVFVWQGTLTLAARFLLPTLERALLVDTLTATAGMLIFAVALVILRLRR
                     IELADYWPSLLYAPLLTLLFR"
     gene            complement(3797..4789)
                     /locus_tag="PKK20_02085"
     CDS             complement(3797..4789)
                     /locus_tag="PKK20_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU98710.1"
                     /translation="MSALIDLVFHSDGRFTMPILTYPGAPLVGATVRQMVTDPKALFE
                     VLAALHRKFATPVVLTAMDLSVESEAFGCEVAFADHEVPTVVGRLVTTRGAAERLAVP
                     EPGAGRTRVYLETARRLRTLPGRPLVLASMLGPFSLAARLYGVSEALGLTIENTDLAH
                     LLLEKATAFLAAYAKAFKAAGADGLVMAEPTAGLLSPRAMIAFSSAYIRRIVESVDDD
                     TFTLILHNCAAKLIHLDAALQAGARVFHFGAPMDLPGALAKAPSDVVLAGNLDPSGVF
                     ARSTPEQVRAATRALLDATRTSRNYVLSSGCDIPPQTPIGNIEAFFAVARGAAC"
     gene            complement(4942..6420)
                     /locus_tag="PKK20_02090"
     CDS             complement(4942..6420)
                     /locus_tag="PKK20_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNU98711.1"
                     /translation="MKEALSLVRIPSLKGVGDFRRHAASLGLEMPCDDAILVGSASPL
                     AQPVADIAINGKRLTNRWAVQPMEGWDGTTDGGVTDEVRRRWRRFGESGAKLICGGEA
                     MAVRPDGRANPHQLVIDDAHRAGLAELCRIARRAHREQYGGADDLVIGFQLTHSGRFC
                     RTRENQPPEPRVAYRHPLLDRKFGVSVDAQVMTDTEVESLIGEYAAAARIAAEAGADF
                     VDLKHCHGYLLHEFLGAHMRPGRYGGSFENRTRILRELVAAIRTSGTRIDLGVRLSAF
                     DCVPFKPDPAGSAPGKPGPGVPEDFSGCLPYRYGFGIDPLDPLKPDLSEAFRFMALCG
                     ELGIKVLNLTAGSPYYSPHIQRPAAYPPSDAYRSPEDPLAGVARHLWAVRQLKAAAPA
                     GLLIVGSAYSYLQDYLPHVAQAVLREGWVDVVGVGRAVLSYPRMLADAVEAGRLQTKC
                     ICRTFSDCTTAPRNGLISGCYPLDRYYADKPEAAALKRMKRR"
     gene            complement(6417..7883)
                     /locus_tag="PKK20_02095"
     CDS             complement(6417..7883)
                     /locus_tag="PKK20_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNU98712.1"
                     /translation="MSLKIDTQVTAHALRARIERLFDLSAAKILSIEGGTSPEAGAPV
                     HTVRGRYTARGWTEWTQGFQFGSALLQFEADGDTRFLELGRRQTMARMATHVSHVGVH
                     DHGFNNVSTYGNLWRLMREGRIPFDQRENDAYELALKVSGAVQAARWTRTADGSGFIH
                     SFNGPHSLFIDTMRTLRSLAIAHQLGHVLMGEQDVRVSLLERLIRHAQVTATYNVFYG
                     EGDGPYDVCGRVAHESVFNPANGVYRCPNSQQGYSPFSTWTRGLAWAILGFAEQLEFL
                     AALPGRSLESELCFHGRPPAVEGAFASLKRTRRLFTVGALTPNEIRFWMTRAATVTAD
                     FYLENCCADGIPMWDTGAPNLHRLGDYLDRPSDPYNPWEPVDSSAAAIAAQGLLRLGR
                     HLDRTGRCREGRRYRQAGLTVARTLFDEPYLSTRPRHQGLILHSVYHRPNGWDHIARG
                     QKVPNGESSMWGDYHARELALLLLREIRGEPPLVFFRS"
     gene            complement(7888..8703)
                     /locus_tag="PKK20_02100"
     CDS             complement(7888..8703)
                     /locus_tag="PKK20_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNU98713.1"
                     /translation="MNRVALITGGSRGIGRGIALALARAGHDLVINYARNAEAAQKTA
                     ADCVALAAAGGHTIRARPCPSDIADPAARQRLIDFARTECGRLDLLVNNAGVAPEVRA
                     DLLESGEASFDRVLAINVKGPFFLTQLAARWMIEQARADAEASGPDAPAFAPPQIVFI
                     SSISAYTASSNRGEYCVAKAALSMLTPLFAARLAEHGINVYEIRPGIIATDMTAPVKA
                     KYDALIAEGVTPIRRWGSPGDVGRAVAAIAEGAFPFSTGEVLNVDGGFHLRRL"
     gene            9021..9596
                     /locus_tag="PKK20_02105"
     CDS             9021..9596
                     /locus_tag="PKK20_02105"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98714.1"
                     /translation="MLTAFAGETVVFQDRFEARLADGWRWLREDASDWRMREGALEIH
                     VRPGDAHTVRNALVRSAHDLRQGRHAIEVTVTSLKRPKQQFEQAGITWYTDGKPVFKL
                     VKELVDGQLMIIPGRKSMTNDTVQLRLVVSGNAWTAQYRPDGKGEYQTAATGELPAPL
                     NDEVSLQCYHGPADAEHWIRFDDFRIVRLTD"
     gene            10142..10217
                     /locus_tag="PKK20_02110"
     tRNA            10142..10217
                     /locus_tag="PKK20_02110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10175..10177,aa:Ile,seq:cat)
     gene            complement(10446..11657)
                     /gene="speY"
                     /locus_tag="PKK20_02115"
     CDS             complement(10446..11657)
                     /gene="speY"
                     /locus_tag="PKK20_02115"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNU98715.1"
                     /translation="MAKPKKQHRRYREDLTETPDWLMKKKCPHKANYMSGKRILPKGL
                     TGKEKLPQIVDDIFLAYNSARLKEGCQLFRDKMLEPDVTIGMTLSGALTPAGLGCSCV
                     VPLINAGFVDWIVATGANLYHDMHFALNYPVHVGSFKFDDTDLRENDLVRIYDVIIPY
                     SDALMATDEILRDILIQPEFQKEMGTAELHYLLGKYCVEWERKNGLRNVSVLAAAYRA
                     GVPCYTSSPGDSTIGMNVAGVELRGNKLRVNPSIDVNETTAFVLAAKRSGGKSAVVLW
                     GGGSPKNFMLQTEPQIQEVLRIKEFGQDYFLQVTDARPDTGGLSGATPSEAVSWGKVD
                     PASLPDAVVCYTDTTLAMPILTHYALATHPRRKHRRLYDQRARMVKALTKEYFQHNKV
                     KMIDGSDPVLA"
     gene            12052..12318
                     /locus_tag="PKK20_02120"
     CDS             12052..12318
                     /locus_tag="PKK20_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNU98716.1"
                     /translation="MRLRTRNVSCIGLAAAVGLEQSGHIKGLYVAGIGLIVFSDTLIS
                     FSEFLRRRELNAWILPTYYLAHLCITATVLLARRASDPIESSFP"
     gene            12441..12845
                     /locus_tag="PKK20_02125"
     CDS             12441..12845
                     /locus_tag="PKK20_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98717.1"
                     /translation="MRCSRSTFPGCAFGFAASCRTAGAGPESSREPASGQGFELNAIA
                     AVVVGGTSFTGGIGTMPGTLIGTVIIGILDKGLNQAGVHFSLQYIVKGLVILAAVYLD
                     VQRPGHGTFQLTALGGAGSLNAKHWQQPGPPS"
     gene            12842..14104
                     /locus_tag="PKK20_02130"
     CDS             12842..14104
                     /locus_tag="PKK20_02130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNU98718.1"
                     /translation="MSDSIQQHIGALGIVDVHEHHLPEITSCRQVNLLQLFQQSYAGW
                     TQARPYPLPSESRAGDPMLAAAGPTTWEALAPYLENSGSSTFVRNLVRGVTELYGQGG
                     VLTRGNWEALDAAVREHHGRPGWCGEVLRRAGIERVITDSYSDPLLDALEAFGNNYNS
                     VLRINAFACGWHPESRDHNENSAHALLARLGCKPESFDDYLAALEKLVDSLASRHQVA
                     LKNALAYDRDLSFDEPDEPSARRAWGQVSPSPAERKAFGDLVVDRLCRLAGERDVPVQ
                     MHLGTALIRGSHPLNAAGLVERHPRTRFLLMHLAYPWSRELLAMAFVYRNIWLDLTWS
                     LLLSPSHFKLALHEAIELLPDESRLMIGGDNWHVEETYATMRLARRLIGEVLAEKIAD
                     GYFGLDDARRLAGKILKENAAAFFGLSR"
     gene            14101..14322
                     /locus_tag="PKK20_02135"
     CDS             14101..14322
                     /locus_tag="PKK20_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98719.1"
                     /translation="MTAGAFAARSVRLFRSLSSPECSGFLSSFIGHTPDGEWTLFLAD
                     LSPVGEARLESWGLELEYKVFSVFSAALW"
BASE COUNT         2674 a         4730 c         4541 g         2787 t
ORIGIN      
        1 ctccgaccaa cgcgatcgaa ctgacgcgcc tgctcgaaca acgccgcgtc gacccccaat
       61 cgctccacct gcgcacggtc ctgctctcgc gccagacgga caccgagacc cgcgatctca
      121 tcgccgccgg gctattcgcc gccgtcaaat gcaacttcgg cgtccgcgaa gtctccgatc
      181 ccgggctctg cgtcgagtgc gaatccggcc gattccacgt caacgaggac cacttcctcg
      241 tcgaagtcca ggatggcgag ttggtggtca cgaccctctg tcgggaggcg atgcccatgc
      301 tgcgctaccg gacacgcgtt gcctgcgaga tcagccgcga gaaatgcccg tgtggtcgaa
      361 ccggcgccgt gatccatccc ggaccccggc tggatgggcg gaggttggtc aacgagacgc
      421 cgctctacga acgccaggtc gccgaggtgc tggcccaaac ggaggcagcc ggtcatccgt
      481 tccagatcga ggtctcggac cggcacatcg tggttcggat ggacatgtgc gaggacctct
      541 tctcggacac aatctgggtg gtcgaacgcc tcacccacgt catcgagtcg gaattcctcg
      601 cccgccttgg catcgaagcc cgggtcgtgt tcacgagccc gagacaaaag cacaagcccg
      661 agacagttgc ctgatcgggc aactgagcta atcgcggcgt acctgcgcgg tggcgttctc
      721 gatgagttcc caaaagcgcg ggttgtcctg cagcgcctcg tcttctccca gaggcagatt
      781 ggatcggaac tgaaaatcct tgagtgtgtt ccggctcagg atgcggtgga ccacaagacc
      841 cagttcgtgt cgttcgaaat agaggcggta atcgcccagc cggaatcggt gcagtgtctt
      901 gcccccgcgc cggagtctgc cgaagcgttc gaggtccgtg tgcaatgcct cctgaggcag
      961 gcctcgaaac tcgccgagga tttccagttg cagttccttg ggcatcctgg ctagttccgc
     1021 cgcgctcgtc ggattaaaga tgatctgaaa cggctgcatt caaggcggct cgggtttggt
     1081 agcgcgtatg ggaatcgcac ccatctttcg gccttgagag ggccgcgtcc taactgatag
     1141 acgaacgcgc cgttgaaaag cggcccattg gataatcgcg ccgcccgcga ttgtcaaccc
     1201 acagtcgaaa aaaagcgaag gccgctctcg cgagcggcct tcgtggaaga actatgccgt
     1261 gagccaggtt agctgcggcg gcggatcagg aacgcgccca tcccgagcag gcccagagcg
     1321 agcacggaag gctcaggaat gatgatttcc ttggtcagcg ccccagcgta attcaactgg
     1381 ccagccgggc cgggaggcgt taagtcgtca ccacccaacg tcgcctggta ggtgccccat
     1441 ttgcctgccc acaggccggc gacatcatag gaagagccct tgtaggcacc caccatgaag
     1501 ttgacggttg caccttgtgc gaacccgtcg agcttccacg tggatcccgc gttggcatac
     1561 ccagtgacgc cagtgaagcc ggccgtgacc ggttcaagag cgccagcgcc acccaccttg
     1621 taacccatga cgaccgcacc cgtctcggtg aacaaaccga aggtccaccc tgccgcggcc
     1681 gagacctcct ccaccgggtt cacgacgacc ttcatattgt tggccccact gtagtatccc
     1741 ttgaactgaa gggtaccacc ggtggacgta tcagcagcca taactgctcc ggccaccaac
     1801 acgcttgcta gtgcaagtaa cgttttcttc atagtgtttt agtctttcga ttctgttctc
     1861 tatgctttct cttttgtctt cccgctacat ccggcagtta ttgcaccgtg aacgtccttg
     1921 tccatgtctt cgccgcagtg ctcaacaccc agaaggcctc gccaacttcg atcgtcggtt
     1981 cgccgttgtc ccagccaaac atgtcactga acgaattcgg cagagcgtat ccgccacctc
     2041 gttggtgctt gtagacagtg tctccgtcct gaatggggaa cgccaacgga tcaaccaacc
     2101 cgcccgcctg aggcaccgga gaagacacca tgttgaaacc cgccttcacc ggaaccgaca
     2161 gagcgccctg gttgacgtca ccgacaaacg taacagtaac gtccgcagag ccagccaaaa
     2221 acacgaacac accttcgccc ggaacgaggg tttgacccgg agtatcccag ccgaacatgt
     2281 cgctgaacgc gttgaccgcc tcatatcccc gttgacgaac ttgtaaactg ccgagccgtc
     2341 cggcaacgca gagccgaaga tagtcccgac ggtgttcccg ccggtcgctg tgttgtttag
     2401 cgggttgctg atcaggttga agccaggctt cagcgtcgag ttcacataac ccacgacgtt
     2461 gaccgaatag acctgcgcgg acaccgtggc ggcacccaca aggcctatcg cagccgttac
     2521 taatgctgtt ttagttctca tatgttgttt ttgttgtttc cgtgtgtgcg cgcaatttca
     2581 caagtccgcc agcccctgtc aatgtttttt tggctttttt tgaacgatgc cggcgccaat
     2641 tcatgaagtc gaaaaggccg gagttgagac ggctcgtccc ccgccatgtc aagtgaagac
     2701 ggcgtgtgtc ggcgacgggg ccctgcctgc cctgcctgcc ctgcctgccg acaggcaggc
     2761 ggaacggtgt agccccgccc agttgcatac ctgcacagat gcggcccgcc aggtcgaaag
     2821 aggactccgc tccccccgca gacgagtcct tgatcggggg ggcggacata tcttctcccg
     2881 agaccatatc cgacacgtct ttgttcttgt ttagccattc attttcaaca tcttgcattc
     2941 ttgtgccgac cggtgcctct ggaggtcgtt gtgttgctcg ttggtgcgat gtcgcccggc
     3001 gatggcaccg cgtgcaccca cttgacttgg tatcacgctt ttctttgtct tgtggccgac
     3061 gcccaattac gcttgagccc gtgatcgggg ctttgctcaa cgcagtcggc atcgtggtcg
     3121 gcgggctcgc gggttcatgg agcgctcgcc agttgtcgcc tcagcgtcag cgccagttgc
     3181 aggtcttgct cggcgccttc acggtctttt tcggcctgag cattgtctgg agcggcctgc
     3241 gggggtcatg gacccaattc ctgggcaagt tcgcactgat gctcgccgcc ctgatgcttg
     3301 gcaaattgac cggacacctg ctgcgcatcc agcgttgcgt caacaaggcc ggcaggttcg
     3361 ccggcaacca actcagtccc gatcaggctc agtccaacag gagtttcagt gacgtctttg
     3421 cggcctgtgc cgcagtctat tgtctcgccc ccctggctct cctgggatcg ttggccgagg
     3481 gtcttcatgg ggatcttcgc ccactgatca tcaaaatgat catggacggc ttggcggcca
     3541 tgtcctttgc gcggatgctc cccggcgccg tgctgctcag cgcgattcca gtgttcgtct
     3601 ggcaggggac actgacgctt gcggcgcgct ttctcttgcc cacactcgag cgagccctgc
     3661 tggtcgacac cctcaccgcc accgcgggca tgctcatctt tgcagtggcc ctggtgatcc
     3721 tgcgcttgcg ccggatcgag ttggcggact actggcccag cctcctctac gccccgctcc
     3781 ttaccctgct gtttcgctaa caagccgcgc cacgggcgac agcgaaaaag gcctcgatgt
     3841 tcccgatcgg cgtctggggc gggatgtcgc atcccgagga cagaacatag ttgcgggacg
     3901 tccgtgtggc gtcgagcagg gcgcgcgtgg cggcgcgaac ctgttcgggc gtggaccggg
     3961 cgaacactcc cgaggggtcg agattgccgg ccaggacgac gtccgaaggg gctttggcaa
     4021 gggcgcctgg caaatccatg ggcgcgccga agtggaagac gcgagcgccc gcctgcaggg
     4081 cggcgtcgag atgaatgagc ttggcggcgc aattgtggag gatcagggtg aaggtgtcgt
     4141 cgtcgaccga ctcgacgatc cggcggatgt atgccgagga aaaggcgatc atcgcccgcg
     4201 gagagagcag gccggcagtc ggttcggcca tcaccaagcc gtcggcgccg gccgctttga
     4261 aggccttcgc gtacgccgca agaaacgcag tggccttttc caggagcagg tgtgccaggt
     4321 ccgtgttctc gatggtcagg ccgagcgcct cgctcacgcc gtacaaccgc gcggccagag
     4381 agaacggacc cagcatcgac gcgagcacca acgggcggcc aggcagggtc cgcagccgcc
     4441 gcgcggtctc caaatacacg cgggtccgtc ccgcgcccgg ttcgggcacc gccagtcgct
     4501 cggctgcgcc gcgcgtcgtc accaaccgcc cgaccaccgt cgggacctcg tggtcggcga
     4561 aagcaacctc gcacccgaac gcctccgact cgacgctcaa atccatggcg gtgagcacaa
     4621 cgggtgtggc aaacttccgg tgcagggcgg ccaggacctc gaacagcgcc ttcggatcgg
     4681 tcaccatctg tcgcacggtg gctccgacca gcggagctcc gggatacgtc aggatgggca
     4741 tcgtgaaacg cccgtccgag tggaacacca agtcgatcaa cgcactcatt tgtttccgat
     4801 ccgtttgccc gcacgcctta gcccccattt ctcatcaact gttgatgtca tctgaccggt
     4861 catcgtcacc tttcacctga aatgcgggct ctcgcatgtt tcacaccccg acggtgcacg
     4921 gggcgatccg cggctcctct atcatctccg tttcatcctc ttcagcgcgg ccgcctccgg
     4981 tttgtccgcg taatacctgt ccagcggata gcatccgctg atcaggccgt tgcgcggcgc
     5041 cgtcgtgcag tcgctgaacg tgcggcagat gcatttggtc tgcaaccgcc ccgcctcgac
     5101 ggcatccgcc aacatccgcg gatagctcaa cacggcgcgg ccaacgccca cgacgtcaac
     5161 ccatccctcc cgcaacacgg cctgcgcgac gtgcggcagg taatcctgca ggtagctgta
     5221 ggccgacccg acgatcagca atccagccgg ggctgccgct ttaagctgtc gcacggccca
     5281 caggtgccgc gccaccccgg ccaacgggtc ttcgggcgat cggtaggcgt cgctgggtgg
     5341 atacgccgcc gggcgctgga tatggggaga gtaatagggc gatccggcag tgaggttgag
     5401 caccttgatt cccagctcgc cgcacaatgc catgaaccga aacgcctccg atagatcggg
     5461 cttgagcggg tcgagcggat cgatcccgaa accgtaccga tacggcagac acccgctgaa
     5521 atcctcgggc acacccggcc ccggcttgcc cggcgcggac ccggccgggt cgggtttgaa
     5581 cgggacacag tcaaaggcgc tcaagcgcac gcccagatcg atccgggtcc cgctggttcg
     5641 gattgcggcg accaactcgc gcagaatgcg ggtgcggttt tcgaagctgc cgccataccg
     5701 gcccgggcgc atgtgcgcgc ccagaaactc gtgcagcaaa tacccgtggc agtgcttcaa
     5761 atccacaaag tcagcccccg cctcagccgc gatccgcgcc gccgcagcgt actccccaat
     5821 caacgactcg acctcggtgt cggtcatcac ctgcgcatcg accgagacac cgaacttgcg
     5881 atcgagcaat ggatgccgat aagcgacccg cggttcaggc ggctggtttt cgcgcgtccg
     5941 gcaaaagcgc cccgaatgag tcaattgaaa gccaatcacc aggtcgtccg cgccgccgta
     6001 ctgttcccgg tgcgcgcgtc gggcgatccg gcagagctcg gccagacccg cccggtgagc
     6061 atcgtcgatg acaagctgat gcggattcgc ccgtccgtcg ggccggaccg ccatggcctc
     6121 gccgccgcag atcagcttgg ccccgctctc cccgaagcgc cgccatcttc gccgcacctc
     6181 atcggtgacg ccgccatccg tggtgccgtc ccatccctcc atcggttgca cggcccacct
     6241 gttggtcagc cgcttgccgt tgatcgcgat gtccgcaacg ggctgggcca ggggggacgc
     6301 cgacccgacg aggatcgcgt cgtcgcacgg catctcgagt cccagagaag cggcgtgccg
     6361 gcgaaagtcg ccaacgccct tgagcgaagg aatccggacc agagacaacg cctctttcac
     6421 gatcggaaaa acaccagagg cggttcccca cggatttccc ggagcagcag cagcgccagc
     6481 tcgcgcgcat ggtaatcgcc ccacatcgaa ctttcgccgt tgggcacctt ttggcctcgt
     6541 gcaatgtggt cccatccgtt gggacgatga taaaccgagt ggaggatgag gccctggtgg
     6601 cgtggccgcg tcgagagata cggctcgtca aagagcgtgc gcgccacggt cagaccggcc
     6661 tggcgatacc gccggccctc gcggcaccgt ccagtccggt cgaggtgccg tcccaatcgc
     6721 aacagaccct gggcggcgat cgcggccgcc gagctgtcga ccggttccca cgggttgtac
     6781 gggtcggacg gtcggtcgag gtaatcgccc agccgatgca ggttcggcgc gcccgtgtcc
     6841 cacatcggaa tgccgtccgc gcagcaattc tcgagataga agtcggcggt gaccgtggcg
     6901 gcgcgggtca tccagaagcg gatttcgttt ggcgtcaagg ccccgacagt gaagaggcgt
     6961 cgcgtcctct tcaagctcgc gaaagcgcct tcaaccgcgg gaggcctgcc gtggaaacag
     7021 agctccgact cgagcgatcg gccgggcagt gcggccagaa actcgagttg ctcggcaaaa
     7081 ccgaggatcg cccacgccag gccgcgcgtc caggtgctga aaggcgagta gccctgctgc
     7141 gagttggggc agcggtaaac cccgttggcg gggttgaaca cgctttcatg cgcaacgcgc
     7201 ccgcacacgt cgtacggacc atcgccctcg ccgtagaaaa cattgtaggt cgccgtgacc
     7261 tgggcgtgcc ggatcaggcg ctcgagcagc gaaacccgga catcctgttc gcccatgagc
     7321 acgtggccca gttgatgcgc aatggccagc gaacggagcg ttcgcatggt gtcgatgaac
     7381 agcgagtgcg gaccattgaa actgtggata aaaccgctgc cgtccgcggt ccgggtccag
     7441 cgcgccgcct ggacggcgcc gctgaccttc aaggccaact cataggcatc gttctcgcgc
     7501 tggtcgaacg ggattctgcc ttcgcgcatg agacgccata aattgccgta cgtcgagacg
     7561 ttgttgaatc cgtggtcgtg cacgcccaca tgcgacacgt gcgttgccat gcgcgccatg
     7621 gtctgccgac gccccagttc gaggaaccgc gtgtcgccgt ccgcctcgaa ctgcagcaga
     7681 gccgagccga actgaaaccc ctgcgtccac tccgtccacc cccgcgccgt gtaacgcccg
     7741 cgcacggtgt gcacgggagc gcccgcctcc gggctcgttc ccccctcgat ggacagaatc
     7801 ttggcggcgg acagatcgaa gagacgctcg atgcgcgccc gcaacgcgtg cgccgttact
     7861 tgggtgtcga tcttgagcga catagcgcta gagacgccgc aggtgaaagc cgccgtcgac
     7921 gttcagcacc tcgcccgtgc tgaacggaaa agcgccctcc gcgatggccg ccacggcccg
     7981 gcccacatcc ccagggctgc cccaacgccg gatcggcgtc acgccctccg caatcagcgc
     8041 gtcgtacttg gccttcacgg gcgcggtcat gtcggtcgca atgatgccgg ggcggatttc
     8101 atacacgttg atgccgtgct cggcgagccg cgcggcgaat agcggcgtca acatcgagag
     8161 cgccgctttg gcgacgcaat actcgccccg gttggaggac gcggtgtaag cgctgatcga
     8221 tgaaatgaac acaatctgtg gcggcgcgaa cgccggggcg tccggcccgg acgcctccgc
     8281 atccgctcgg gcctgctcga tcatccagcg cgccgccagt tgcgtcagaa aaaaagggcc
     8341 cttgacgttg atcgccaaca cccggtcgaa actggcctcg cccgactcga gcaagtcggc
     8401 ccgcacctcg ggggccacac ccgcgttgtt gaccagcaaa tcgagacgcc cacactccgt
     8461 ccgggcgaaa tcgataagtc tttggcgcgc ggccgggtct gcaatgtcgc tcgggcaagg
     8521 ccgcgcccga atcgtgtgtc cgccggccgc ggcaagcgcg acgcaatccg ccgccgtctt
     8581 ctgtgccgct tcggcgttcc gcgcgtagtt gatcaccagg tcgtgccccg cacgcgccag
     8641 ggcgagcgca atcccgcgcc cgatcccgcg cgacccgccg gtaatcagtg ccacccggtt
     8701 catggcatcg aaactagtcc gccggcccac acccggaaaa gcagaaaaca gcgcggcaac
     8761 aaggtgagcc gggtcaaccc tcgatgaacc gagccaatgg ggtcgcatct aaatagttga
     8821 caattcattc gaagttgaat tggaacgact gcaaatcacc accaatggcg ccaaggacac
     8881 gaacgacacg aacgacacga agacggacgg cgtgtcgaaa aggcggcttg cgagattgac
     8941 aaccgctccg cgagcgaaaa ccctgacatc actctatgag aactccggcg ggaatcgctt
     9001 tcttggtgct cggcacggct atgttgactg cgttcgccgg cgagacggtg gtctttcagg
     9061 accggtttga ggcccggctt gcggacggct ggcggtggct gcgcgaggac gcgtccgatt
     9121 ggcggatgcg cgaaggggcc ctcgagattc atgtccgccc gggcgacgcc cacacggtgc
     9181 gcaacgcgct ggtgcgctcg gcgcacgacc tccgtcaggg gcgccacgcc atcgaggtga
     9241 cggtcaccag cctcaagcgg ccaaagcagc agttcgaaca agccggcatc acctggtaca
     9301 ccgacggcaa acccgtcttc aaactggtga aggaactggt ggacggacag ctcatgatca
     9361 tcccgggacg caagtcgatg accaacgaca cggtccaact ccggctcgtt gtgtccggca
     9421 atgcctggac cgcgcagtac cgtcccgacg gcaaaggcga gtaccagacc gcggcgacgg
     9481 gcgaactgcc cgcgcctctg aacgacgagg tcagcctgca gtgctatcac gggcctgctg
     9541 acgcagagca ttggattcga ttcgacgact tccgcattgt ccggctgacg gactagccgg
     9601 gatcaggttc agcgcggagc ctgtgaaggt ccggcgacgt catcaacctc agcgccaaca
     9661 cggacgccga cggacggcag tggaacggcg cttcctggcg actggtgccg ccgcttcgtt
     9721 tgcgtgcagg ggagtccgcc ctggctgcac cttgcgactt ttcgagcgcg ggtttcagct
     9781 ccgcgacggc tgcttcagcc tccttgacgc ggcgttcgag gtcgtcgcgg gtcaagcatc
     9841 aagagccgga tgaacgccgt gcatgcctgc cattgaagtc cgccaggccc agcttggcga
     9901 cggtgaagac gccggtcttc aagccctccc gaaacagctt gccgccgacc cggacttggg
     9961 caaagtaagt gccggatgat ttccagcgca gtaccggagt ccgctgccaa gggccgggtt
    10021 gttgcttggt cgggcctgcg gattggcgat cgccggacat ggaggcagta cgagaatgca
    10081 gtccgcgatt gtcgaatctc ctgttgttgc gtatatgtaa tcgattggca gtcaatttga
    10141 tgggcccata gctcaggggt tagagcaggc gactcataat cgcttggtcg ggggttcaaa
    10201 tccctctggg cccaccatac ttacgcaaac tggccaaggt ctcttggcct cttcttggcc
    10261 acttttccag cgcccttggc ggtcgtcccg aatcgaacac gcccgcgcct cggctccggc
    10321 gagtggtgcc ggaccagcag ttcgtcctgc aagggtctgt ctatctccga ttgctgcgtg
    10381 atgagagcgg acaccgaaac ccgttagccc gttacagttc ctcgcgcagc gtatgaaaca
    10441 actggctaag ccagcaccgg atccgagccg tcgatcatct tgaccttgtt gtgttggaag
    10501 tactccttgg tcagggcctt aaccattcga gcgcgctggt cgtagaggcg tctgtgcttg
    10561 cgtcggggat gtgtggccag cgcgtaatgc gtgagaatcg gcatggccag ggttgtgtcg
    10621 gtgtagcaca cgacggcatc gggcaggctc gccgggtcga ctttgcccca gctcacggct
    10681 tcgctcgggg ttgcgccgct gaggccgccg gtgtcgggcc gcgcgtcggt gacctggagg
    10741 aagtagtcct ggccgaattc cttgatgcgg aggacttcct ggatttgggg ttcggtctgg
    10801 agcatgaagt tcttcgggct gccgcctccc cagaggacga cggcgctctt gccgccgctg
    10861 cgcttggctg caagcacgaa agccgttgtc tcattcacgt cgatcgaggg attgacgcgc
    10921 agcttgttgc cgcgcagttc aaccccggcc acgttcatcc cgatggtcga gtctcccgga
    10981 gacgacgtgt agcagggcac gcccgcgcgg taggcggcgg ccaggaccga gacgttcctg
    11041 aggccgttct tgcgttccca ttcgacgcag tatttgccca ggagatagtg cagttcggcc
    11101 gttcccatct ccttttggaa ctcgggttga atcaggatgt cacggaggat ctcgtccgtg
    11161 gccatgaggg cgtcggagta agggatgatg acgtcgtaga tgcggaccag gtcgttctcg
    11221 cgcagatcgg tgtcgtcgaa tttgaagctg ccgacgtgca ccgggtagtt cagggcgaag
    11281 tgcatgtcgt gatacagatt ggcgccggtg gccacaatcc aatcgacgaa cccggcattg
    11341 atcagaggaa ccacgcagga gcagcccagg ccggcgggag tgagcgcgcc agacaacgtc
    11401 atgccaatcg tcacatccgg ttcgagcatc ttgtctctga acaactggca gccttccttg
    11461 agtcgggcgc tgttgtacgc caggaagatg tcatccacga tctgcggaag cttttccttc
    11521 ccggtcaagc ccttgggaag gatgcgtttg cccgacatgt agttcgcctt gtgcgggcac
    11581 ttcttcttca tcaaccaatc gggcgtctcg gtcaggtctt cgcggtagcg acggtgttgt
    11641 tttttgggtt ttgccataaa ttccagttgc tcggctcgtt attggaatct ggaggatacg
    11701 ggcgcatgcc gggaagccaa ccttttttcg tcaatctctc ttcgtcaatc cgccaatccg
    11761 cggtctgatg catctggttg ttgtcggtgt tctgtcgccc ggtgagggca ccgggccgac
    11821 acgtggtgga gactcccggt gtagacgtcg ccgcgtagcc tgcctgcgcc gcaaagcgca
    11881 gcggcctgtc tgccgtgtgg ggcacggcac aggcaggcgc ggcaggcagg ccccacgcgg
    11941 cgcaaactcc attccaatcg acaaacccca gatgcaccgt cggcagtctt gcatgttggc
    12001 ggtcttgcat ttccagcccg cacatttgac gctctgtctg tgcacgtgcc gatgagactt
    12061 cgcacgagga atgtctcctg catcggattg gctgccgcag ttggcctcga acagtcgggg
    12121 cacatcaagg ggctttacgt cgccggcatc ggcctgattg tcttctccga cacgctgata
    12181 tcgttcagcg aattcctccg ccggcgcgaa ctcaacgcct ggattctgcc cacgtactac
    12241 ctggcgcacc tgtgcattac ggcgaccgtt cttctggcga ggcgcgcctc agacccaatc
    12301 gagtcctcat ttccatgagc agcctccgtg tttcctcgcc ctaacccaca tacattacac
    12361 cccaatggtg cacaggaaag agaccatcga gggggcgcgc ccggtcatgc gcgatttgct
    12421 gggccggttt ccggagccgg atgcggtgtt cccgatcaac gttccctggg tgcgcgtttg
    12481 gattcgcggc atcgtgccgg acggccggcg ccgggccgga atcgtctcgg gagcctgcgt
    12541 ccggtcaggg cttcgaactc aatgccatcg ccgccgtggt cgtcggcggc acgagcttca
    12601 ccggcgggat cgggaccatg cccggaacgc tcatcggcac tgtcattatc ggaatcctcg
    12661 acaaaggact caaccaggcc ggcgtccatt tctcgctgca atacattgtc aagggacttg
    12721 tcattctggc cgccgtctat ctcgacgtgc agcggccagg acatggcacg ttccaattga
    12781 ccgctctcgg cggagcgggc agcctcaatg ccaagcattg gcaacaacct ggaccaccat
    12841 cgtgagcgac tcgattcaac agcatattgg cgcgctcggc attgtggacg tgcatgagca
    12901 tcatcttccg gagatcacct cgtgtcggca ggtgaacctc ctgcagttgt tccagcaaag
    12961 ctacgcgggc tggactcagg cgcgtccgta cccgctgccc tcggagagcc gcgcgggcga
    13021 tccgatgctc gcggcggccg ggccgaccac gtgggaggcg ctcgcgccgt acctcgagaa
    13081 cagcggatcg agcacgtttg tgcgcaacct cgtgcggggc gtcacggaac tgtacggcca
    13141 aggcggcgtg ctgacgcgcg ggaactggga ggctttggat gccgccgtgc gcgagcatca
    13201 tggccgtccg ggttggtgcg gggaggtgct gcggcgggcc gggatcgagc gggtcatcac
    13261 cgattcatac agcgatccgc tgctcgatgc actcgaggcc ttcggcaaca attacaactc
    13321 cgtcctccgg atcaatgcct tcgcgtgcgg gtggcatccg gagtcgcggg atcacaacga
    13381 gaactcggcg cacgccctgc tggccagatt gggctgcaag ccggagtcgt tcgacgatta
    13441 cctcgcggcc ctcgagaagc ttgtggattc cctggcttca cgccaccagg tggccctcaa
    13501 gaacgccctg gcgtacgatc gcgacctgag ctttgacgaa ccggacgagc cgtccgcgcg
    13561 ccgggcctgg ggccaggtgt ccccttcgcc cgcggagcga aaagcctttg gcgacctggt
    13621 tgtcgatcgg ctctgtcggc tggccggcga acgcgatgtg ccggtgcaga tgcatctggg
    13681 cacggcgctc atccgcggct cgcatccgct caatgcggcc gggctggtcg agcgtcatcc
    13741 gcgcacgcgc ttcctgctga tgcatctggc gtatccgtgg agccgcgaac tgctggcgat
    13801 ggcctttgtc tatcgcaaca tctggctcga tttgacctgg tcgctcctgc tgagtccgtc
    13861 ccacttcaag ctggcgctgc acgaggcgat cgagctgttg cccgacgaat cgcgcctgat
    13921 gatcggcggg gacaactggc acgtcgagga gacgtacgcc acgatgcggc tggcccgacg
    13981 actgatcggc gaggtgctgg ccgagaagat cgccgacggg tatttcggac tcgatgacgc
    14041 gcggcgattg gcgggcaaaa tcctcaagga aaacgcggct gcgtttttcg gactgagccg
    14101 gtgacagcag gggcttttgc agcgcggtcg gtccgtctgt ttcggtcctt gtcttcgccg
    14161 gagtgctcgg gcttcctgtc gtcgttcatc ggccacactc cggacggtga gtggacgctg
    14221 ttcctggccg atttgtcgcc ggtcggcgag gcgcgactcg aatcctgggg gctcgaactc
    14281 gagtataagg tcttctctgt gttctctgcg gctctgtggt gaaatcccgt cgatgactgt
    14341 caccgtcccg aattgaacca cagagacaca gagcacacag agaaggcctc ttcaatgtcg
    14401 agcgggaaca tccgaggaac cggcgagtgt ggtgtggaac agggttccca ggatcgactg
    14461 gatttcaatt ggtcttctct gtgaactctg cggctctgtg gtgaatcccg tcgatgacag
    14521 tcaccgtccc gaattgaacc acagagacac agaggacaca gaggacagcg catccaaaga
    14581 gccacagatg aaattctgtg gattaaggtc ttctctgtgt tctctgcgtc tctgtggtga
    14641 aatcccgtcg atgactgtca ccgtcccgaa ttgaacccac cgacgcaccg acgcaccgac
    14701 gcaccgacgc accgacgcac cgacgcaccg ac
//
LOCUS       DAOQRO010000029        14683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3168_length_14683_cov_5.767774, whole genome shotgun sequence.
ACCESSION   DAOQRO010000029 DAOQRO010000000
VERSION     DAOQRO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_14683_cov_5.767774"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /gene="grpE"
                     /locus_tag="PKK20_02140"
     CDS             <1..1766
                     /gene="grpE"
                     /locus_tag="PKK20_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNU98720.1"
                     /translation="PMSAIEVCAFAACVALGAMLAVWPFRLRHHAELKLVETDGLADT
                     LRQIEQVQDVADRIETATGQWQTAHEHAAKTVAAAREVMDRMTEEQRAFQAFMQRAED
                     SERHHLRLETTKLRRAEGDWLQVLVRILDNVYALYLAGVRSGQPRLIHEFTQFQNACR
                     DAARRVGMVPIAAAPGTPFDPERHQVSDANATVPKGATVLETMAIGHLFQGQLLRRAL
                     VTLQPQAAAPASTTSTEALPQPPPPPSAGDFQDKPASPEDSVSSGAPQAIDGPEETHP
                     IEPNAPREAASEDVEIAEAGPEPKAATAPEQAAGGSFLGLVEFAPSFTPRPGISHVLF
                     DFDGTLSLIRQGWPDVMVPMFVEMLPAQPGETPEALRRLAFEDIMRLNGKQTIYQMIQ
                     LVERIRERGGEPREPLWYKHEYLRRLDARIRHRIDALASGTLEPDDLLVFEARPFLDE
                     LRRRGFSLYLASGTDEVFVKQEAAALGLTQYFGPRIYGALDDYKQFSKKMVIDRILRE
                     NQIDGARLLSFGDGYVEIQNTKEVGGLAVAVASDEANNGSGQCDEWKRQRLLGVGADV
                     VIPDFRDALTLLPILLGEPES"
     gene            1839..2984
                     /locus_tag="PKK20_02145"
     CDS             1839..2984
                     /locus_tag="PKK20_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98721.1"
                     /translation="MSDSTEVCGTSGLNLRRVRVLPLEQRQSLSRLEEILVEPSNDPP
                     PLSDANARLVRECAARIREARQRGASVMLIYGAHLIKNGGQLLLVRLLDHGYLTHLAT
                     NGAGTIHDWEFSFLGRSTESVRANVATGTFGTWDETGRFIHLALLLGGLRGEGYGQAL
                     GRFIYQDGGQVPKADDLEAQVRADPHHPLTPARVELLHAIRQFGIASGPLMIQHRWRN
                     ASVLANAFRHQIPLTVHPGIGYDIIATHPMFNGAAIGRAGGIDFARFATSVEGLDGGV
                     AMSIGSAIMGPQVFEKTLSCVNNLRIQAGRPIVQGHTLYVVDLQDGGRWDWTRGEPPR
                     DNPAYYLRFCKSYARMGGTMHYAQCDNVAFLHHLYRTLADKPNTKTP"
     gene            2981..4030
                     /locus_tag="PKK20_02150"
     CDS             2981..4030
                     /locus_tag="PKK20_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNU98722.1"
                     /translation="MTPERFSTLTGRYSGLRVAVAGDFCLDRYLEIDPGRTETSLETG
                     LPVHNVVRVRGQPGAAGTILNNLVALGLGTLYPVGFCGDDGEGFELRRALAGMPGVRM
                     DFFVTTGARRTFTYCKPIAIEPGRPPVEFNRLDSKNWTPTPRDIERRLAEGVETLAPQ
                     IDALVLLDQVDLADTGVVTRGVLASVNRLVADHPRLPILADSRRGLRGFPPVIFKMNA
                     HELAALTGGAADVADAASVRQAALTLARSHGRPVFVTLAEHGMIGACPDGASYHVPAL
                     PLRGDIDIVGAGDAVTANLAAALAAGASVREALELASLAASIVIHQLGTTGTASVAQL
                     RDLHTRSVQGSHLHS"
     gene            complement(4216..4629)
                     /locus_tag="PKK20_02155"
     CDS             complement(4216..4629)
                     /locus_tag="PKK20_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNU98723.1"
                     /translation="MNTVFVDTGAFVARVLPRDQHHAASRRGWETLEHVDVRLYSSEH
                     VLDEAVSLLIRQAGAEYAARWARDHLASEEIVWLNTSREDWHDALRWLEKFSDQKLSF
                     TDCLSFSLMRREGIPSVFGFDRHFALAGYELWPRL"
     gene            complement(4626..4907)
                     /locus_tag="PKK20_02160"
     CDS             complement(4626..4907)
                     /locus_tag="PKK20_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNU98724.1"
                     /translation="MTRTSIMLSEEVKRRAMAEARKLGVSFADFVRQAIAEKLPHQGK
                     GVDRLKRRRQDPLFRLCDRLPVASGVTATDVAGSHDDYLYGQTPKHAQQ"
     gene            complement(5457..8129)
                     /gene="gyrA"
                     /locus_tag="PKK20_02165"
     CDS             complement(5457..8129)
                     /gene="gyrA"
                     /locus_tag="PKK20_02165"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNU98725.1"
                     /translation="MPEENQTPPPPPPPESPTPLYAANEKVEKINVAEEIKNSFLDYS
                     MSVIVSRALPDVRDGLKPSQRRILFAMFHDLGLLPTRRHLKCARIVGETMGKYHPHGD
                     QAIYPTLVHMAQPWSMRDTLVDGQGNFGSVENDPPAAMRYTEARMTHLGAVLMEDIDK
                     QTVDFAPTYDEEHEEPLVLPAAFPNLLVNGGTGIAVGMATNMAPHNLGEVVDAICAQI
                     DNPGITIPQLMKHIKGPDFPTGCMVCGLEGIRQYFGTGRGSVKIRGRVGVEQLKGGRE
                     QIIVTEIPYNVNRAQLVERIATLVNEGELKEFKDITAVRDESDENTRVVIELKRDAVA
                     KVVINNLYRHTALETSFAVNALAIDHGRPKTLNLKELINCYIEHRREVILRRTRYLLG
                     KAEERAEVLEGYLAAIANLDEFIRIIRTSRTRDEAKVKLLAFEWTRLQVESWGIRIRQ
                     EALLVNGRYSLTERQVDEILNLRLYQLTGLEIDRVAAEYKALLETIHDLLDILAKESR
                     VFAIIKAELQAVKGKNATPRLTDLVPDEGEIAIEDLIANEGVIITMTHNSLIKRTAVS
                     SYRAQRRGGKGVIGMTARENSAVEGDAPDFIERLFTASTHDYLMFFTNMGRVYVERVH
                     EIPDMGRAAKGRSIANLLELQAGEKVTALIRIESKTGSAKEDVTWQQPGYIFFATQSG
                     TVKKTALAEFANVRRGGIIAIGIEPGDTLIDVRYTRGTDEVVLITRGGMSIRFSEEDV
                     RSMGRPATGVRGISLDPEDRVVAAAVVDPEATLLVAGENGLGKRTRFEEYRIQSRGGK
                     GIITMKTTERTGGVLGALTVREVDEVMLITAGGQMVRIAARDIRETGRNAQGVKLIDL
                     DESDRLQGIAPVISEDKEDAVEDNSTPPSAPPAD"
     gene            complement(8154..10655)
                     /gene="gyrB"
                     /locus_tag="PKK20_02170"
     CDS             complement(8154..10655)
                     /gene="gyrB"
                     /locus_tag="PKK20_02170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNU98726.1"
                     /translation="MADNVPTTDKYDASKIDKLEGLEAVRKRPGMYIGDPDERGLHHC
                     VFEVLDNSIDEHLAGFCQRIEVTMHVDGSCSIRDDGRGIPVDTHPKWNMPAVELVLTN
                     LHAGGKFGQGAYKYSGGLHGVGAKCVNALSEWFKVEVSRDGKVYYMEFARGVTVRKLE
                     VIGKSKHTGTLITFKPDPVIFTLTTEFKFEHLALRLRELAFLNPGVEILLTDERTENK
                     SERFFYRDGIEEFVKQLGKSKQTIHPKPVCLSGKRVIKVETREEDAFVDCVLQYTDSY
                     NEQILCFANSIANPDGGTHLTGFRSALTRAINQYAKTNELLKEKDASISGDDVREGLV
                     CVLSVKLPNPRFESQTKVKLVNTEIDGVVSSIVYDGLMTYFDANPSVAKRIIEKCLMA
                     ARAREAARKAREAVRKTALTGGGLPGKLADCSDRDPANTELYIVEGDSAGGSAKQGRD
                     RKFQAILPIRGKLINVEKARLDKVLQNLEIRTMITAVGTGIGDSEGEGAFNLEKLRYH
                     KIIIMTDADVDGAHIRTLLLTFFYRQMPELIRRGFVYIAQPPLCQIARKKRVEYVEDD
                     PQLNRILIQLGSEDVRLRNVADGREFNERQLSEILELLVVLEKYVRALRRHGGDFAEY
                     VEKRRLDTHELPRHLVKVRHGNDEAVHYFHTEEELREFGEANPDLGLFGEEETAAGAD
                     RKNGHNGPTRRARHVELHETKAVEDLLAKLARKGLNVEHFAAQDKPLFELVEGEGERT
                     QVKPIFSIPEILLGVKEVGKRGLQIKRFKGLGEMNPKELFETTMNPARRKLLRIDLTD
                     AVEAEEMFTKLMGDEVEPRRQFIEDNALNVRNLDV"
     gene            complement(10847..11695)
                     /gene="rsmA"
                     /locus_tag="PKK20_02175"
     CDS             complement(10847..11695)
                     /gene="rsmA"
                     /locus_tag="PKK20_02175"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNU98727.1"
                     /translation="MTLSEMRQILTARRIRLTKSLGQNFLHDGNQLRRMVGLAGLRPN
                     DQVIEIGPGLGPLTELLLAGACEVLTIEKDARLVRCLDERFAGDRRLRLVHADAVDFL
                     AGHAEDWASWKLVAALPYSAASEILMTLALAPTCPERLVITLQQEVAQRLSVGPGSPD
                     YGILTLIVRHRYAPAASFSIPASCFFPPPKIATTCLALDRRSGPGFSAFERRTFARLV
                     RSAFSQRRKMMFKLLRSGWDEEALRGAFETLDLSQTCRAESLGLERFEALARLLTPSG
                     GDTQRF"
     gene            complement(11692..13056)
                     /locus_tag="PKK20_02180"
     CDS             complement(11692..13056)
                     /locus_tag="PKK20_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNU98728.1"
                     /translation="MKATRRILDLLRAYESRNVTLIAPDLSGPIVWERARGAHVWDLE
                     GRRYLDLTAAFGVAAAGHANPRVVATARRQMSHLLHAMGDVYPHRLKATLARELSRLT
                     FERWTGGAGESRISDRGRTMIGATRQPPRTGKVIFGNSGFEAVEAALKTALLATGRAG
                     VVAFQGAYHGLGYGALTATERAHFRLPFRSQLNPFVRFAPFPVSDDAAARACAAVRHH
                     LGSGRIGAVLIEPVQGRSGIRVPPSDFLSRVRALCDQFGALLIVDEIYTGFGRTGRWF
                     ACERGGVVPDLVCLGKALTGGFPLSACVGRADVMDRAWPEATGEAIHTSTFLGHPVGC
                     AMALSQIGEIRSRRLPRRSERLGAVLRTRLSRMAVPAGLVGDVRGLGLMVGFELCHRD
                     GRPACDAVCEIVRVMLDRGVLILPEGEHGNVLAFTPPLVISQIDLERAVGALEDALAQ
                     LGSA"
     gene            complement(13057..13791)
                     /locus_tag="PKK20_02185"
     CDS             complement(13057..13791)
                     /locus_tag="PKK20_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU98729.1"
                     /translation="MPDHLTVNEIYLSVQGESTFAGLPCVLIRLTGCNLRCSYCDTAY
                     AFRQGRRRTLEDIRAEVRRLAAPYRAVRRPVSIPLVELTGGEPLLQPGSLPLLEALCD
                     DGYTVLLETSGALDIAPVDPRVRRVMDIKCPSSGESAQNRWENLTHLRATDEVKFVIG
                     TEQDYRWAREVVFERRLDEMCPILFSWVSPVEPRQRDPSLKSIPPGQNPISRRALAER
                     VIADALPVRFQVQLHKVIWPADQRGV"
     gene            complement(14000..>14683)
                     /locus_tag="PKK20_02190"
     CDS             complement(14000..>14683)
                     /locus_tag="PKK20_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU98730.1"
                     /translation="SAPSRPGRRRAASSASPSGDEAKEIADRMRKYLTVAQGEIVRLD
                     YIVTQFLDAIRPTVPQMRPALLNEVAKATLDLLRPELDNRGLAVETHLARDLPEARFD
                     AAQIKQALVNLIKNSMHAMTRGGVLTIRTGTASEGVWISVGDTGTGISQERLSRLFEP
                     FFTTKKKGTGLGMMIVYRIVREHGGRIDVESHPGKGTVFRLWFPLRERGPRLLEAGAV
                     ADQKPAPTA"
BASE COUNT         2554 a         4808 c         4661 g         2660 t
ORIGIN      
        1 ggcccatgtc agccatcgag gtgtgcgctt ttgctgcctg cgtggccctg ggcgccatgc
       61 tggccgtgtg gccctttcgc ctccgacacc acgccgaact caagctcgtc gagacggatg
      121 gcctggccga caccctccgg cagatcgagc aggtccagga tgtcgcggac cgcatcgaga
      181 ccgccaccgg ccagtggcaa acggcgcacg aacacgccgc caaaacggtc gccgcggcgc
      241 gcgaggtcat ggatcgcatg accgaggagc agcgcgcctt ccaggccttc atgcagagag
      301 ccgaggactc cgagcgccat cacctgcgcc tcgagaccac caaactccgc cgcgccgagg
      361 gcgactggct ccaggtcctc gtccgcatcc tggacaatgt ctacgccctc tacctggccg
      421 gcgtgcggtc cggccagccc aggctcatcc acgagttcac ccagttccag aacgcttgcc
      481 gcgatgccgc gcggcgcgtc ggcatggtgc cgattgcggc cgccccgggc acgcccttcg
      541 accccgagcg gcaccaggtg tccgatgcga acgcgacggt ccccaagggc gcaaccgtgc
      601 tcgagaccat ggccatcgga catctgtttc agggtcagtt gctccggcgc gccctcgtga
      661 cccttcaacc tcaggccgca gcgccggcct caacgacatc gaccgaggcc ttgccccagc
      721 caccgccgcc tccaagcgca ggcgacttcc aggataagcc cgcctccccg gaagacagcg
      781 tctcgtccgg cgcgccgcag gcaatcgacg gcccggagga aacgcacccg atcgagccga
      841 acgcgccccg cgaggccgcg tcggaagacg ttgagattgc agaagcgggg cccgagccca
      901 aagccgcaac agcgccagaa caggctgcag gcggctcgtt tctgggattg gtcgagttcg
      961 ccccctcgtt cacgccacgc cccggcatca gccacgtgct gttcgacttc gacggcactc
     1021 tgtcgctgat ccggcagggc tggcccgatg tgatggtgcc gatgttcgtc gagatgctgc
     1081 cggcccaacc gggcgagacg cccgaggcgc tgcggcggct ggcgttcgag gacatcatgc
     1141 ggctcaacgg caagcaaaca atctaccaga tgatccaact ggtcgaacgc atccgcgagc
     1201 gcggaggcga gccgcgcgaa ccgctctggt acaaacacga gtacttgcgc cggctcgatg
     1261 cccgcatccg ccatcgcatc gacgccctcg ccagcggaac gctcgagccg gacgatctgc
     1321 tcgtgttcga agcccgcccg tttctcgacg aactgcggcg gcgcgggttc tcgctctacc
     1381 tggccagcgg gacggacgag gtgttcgtga aacaggaggc cgcggccctg gggctcaccc
     1441 agtactttgg cccccgcatc tatggggccc ttgatgacta caaacagttc tcgaagaaga
     1501 tggtgatcga ccgcatcctg cgggagaacc agatcgacgg cgcccggttg ctctcgtttg
     1561 gcgacggcta cgtcgagatc cagaacacga aggaggtcgg cggcctggcc gtcgccgtgg
     1621 ccagcgacga ggccaacaac gggtcgggac agtgcgacga gtggaagcgc cagcggctgc
     1681 tcggtgtagg cgcggacgtg gtcattccgg acttccggga cgccctgaca ctgcttccga
     1741 ttctcctcgg cgagcccgag tcttaggatc tgcatttctc accactcttg aacaacgtga
     1801 agtcggagta cacaggagaa actcaaatgc aggcccggat gtcagactcc acagaggttt
     1861 gtggaacatc cgggcttaat ctgcgccgcg tgcgcgtgct cccgctcgaa cagcgccaaa
     1921 gcctgagccg gctcgaggag attctcgttg aaccctcgaa cgatccgccg cccctttcgg
     1981 acgccaatgc gcggctggtg cgggagtgcg ccgcgcgcat ccgcgaggcc cggcaacgcg
     2041 gggcgagcgt gatgctcatt tacggcgccc acctcatcaa gaatggcggg cagttgctcc
     2101 tggtgcggtt gctcgatcat ggttatctca cccacctggc caccaacggc gcaggaacca
     2161 ttcatgactg ggaattctcg ttcctcggac gctcgaccga aagcgtccgc gccaatgtcg
     2221 ccaccggcac gttcggcact tgggacgaga caggccgctt catccatctg gcgctgctgc
     2281 tgggcgggct caggggcgag gggtacggac aagcgctggg ccggttcatt taccaggatg
     2341 gcgggcaggt gcccaaggcc gacgatctcg aggcgcaggt gcgcgctgac ccgcaccatc
     2401 cgctcacgcc agcccgggtc gaattactgc acgcaatccg tcaattcggg attgcgtctg
     2461 gccccctcat gatccagcac cgttggcgaa acgcctcggt gctggccaat gctttccgac
     2521 accagattcc tctgaccgtc catcccggaa tcggctacga catcatcgcc actcatccca
     2581 tgttcaatgg ggcggcgatc gggagggcgg gcggcatcga ttttgcccgg ttcgccacgt
     2641 ccgtcgaggg actcgatggc ggcgtggcga tgtcgatcgg gtcggcgatc atggggccgc
     2701 aggttttcga gaagacattg agttgcgtga acaatctccg catccaggcc ggacgcccca
     2761 ttgtccaggg acacacgctt tacgtggtcg atctgcagga tggcgggcgc tgggactgga
     2821 cccggggcga gccgccgcgc gacaaccccg cctactacct ccgcttctgc aagagctacg
     2881 cgcgcatggg cggcaccatg cattacgccc agtgcgacaa cgtcgccttc ctgcatcatc
     2941 tctaccgcac gctcgccgac aaaccgaata cgaaaacacc atgacccccg aacggttctc
     3001 gacactcacc ggacgctact ccgggctccg cgtcgcggtc gccggcgatt tctgcctcga
     3061 ccgctacctc gagattgacc cggggcgaac ggagacatcg ctcgaaaccg gcctgccggt
     3121 gcacaacgtc gtccgcgtgc gcggccaacc gggagccgcc ggcaccatcc tcaacaacct
     3181 tgtcgccctc ggcctcggaa cgctctatcc ggtcgggttc tgcggagacg acggcgaggg
     3241 attcgaactg cggcgcgccc tggccgggat gcccggtgtg cggatggatt tcttcgtgac
     3301 gacaggcgcg cgccgcacgt tcacctactg caaacccatc gcgatcgaac ccggacgccc
     3361 gccggtcgaa ttcaaccgtc tcgacagcaa gaactggacg ccgacgccgc gcgacatcga
     3421 gcggcggctg gccgagggcg tcgaaaccct ggcgccacaa atcgatgcgt tggtcctgct
     3481 cgaccaggtc gacctggccg acaccggagt cgtcacgcgc ggcgtgctgg catcggtaaa
     3541 ccgattggtt gcggaccacc cccggttgcc catcctggcc gacagccggc gcggcctccg
     3601 gggcttcccg cccgtcatct tcaagatgaa tgcgcacgag cttgccgccc tgaccggcgg
     3661 agccgcggat gtcgcggacg ctgcgtcggt ccgccaggcg gcgttgaccc tcgctcgatc
     3721 tcatgggcgc ccggtgtttg tcacgcttgc cgagcacggc atgattggcg cctgcccgga
     3781 cggcgcgtcc taccatgtgc ccgcgctgcc gttgcgaggc gacatcgaca ttgtgggcgc
     3841 gggcgacgcc gtcacggcga atctcgcggc ggcgctcgcg gcgggggcgt cggtgcgcga
     3901 ggcgctcgaa ctggccagcc tcgcggcgtc gattgtcatt caccaactgg gcaccaccgg
     3961 caccgcctcc gtggcccaac tccgcgacct ccatacacga agcgtccagg ggtcacatct
     4021 tcacagttga caattccggg gaagcgtcca ggggtcacat cttcacagtt gacaattccg
     4081 ggaaagaccc agccggagtg aggcccggcc atgcagaacc cgtcgcctgg cctgatccag
     4141 gcggctctcc gaagtcagtg agactcccat ccggaacccg cccgttcgtc attctctggc
     4201 ggagccggtc gcccttcaca atcgcggcca aagctcgtag ccggccagcg cgaaatggcg
     4261 atcgaagccg aagaccgacg ggatgccttc gcgtcgcatc agactgaacg acaggcaatc
     4321 ggtgaaactc aatttctggt cggaaaactt ctcgagccag cgcaacgcat cgtgccagtc
     4381 ttcgcggctc gtgttcaacc aaacgatctc ttcggacgcc agatggtcac gcgcccaccg
     4441 ggcggcgtac tcggctccgg cctgccggat cagcagggac accgcttcgt cgagcacgtg
     4501 ctcgctggag tagagcctga catccacatg ctcgagtgtc tcccatccgc gacggctggc
     4561 tgcgtgatgc tggtcgcgcg gcaggacgcg cgccacgaac gcaccggtat ccacaaacac
     4621 cgtgttcatt gctgggcgtg tttgggtgtc tggccataga ggtagtcgtc atggctgccg
     4681 gcgacatccg tggcggtgac gccgctcgcc acggggaggc ggtcgcacag ccggaacaag
     4741 ggatcctgcc gtcggcgttt gagacgatcg acgcctttgc cctgatgcgg cagtttctca
     4801 gcgatcgcct ggcgcacgaa gtcggcaaag gaaactccca acttccgcgc ctcggccatc
     4861 gcccgccgtt tgacctcttc tgacagcatg atgcttgtgc gtgtcataat ggcataacgt
     4921 ggagtagcgg tcgaaacagg tcaaggccga tcgtcggtca acgacacggt tgctggtcgc
     4981 ctttccaggc ttctcttgct tcacacctgg gcgcggtgtt gaaggtgatg ccgcacctct
     5041 gcaaggcgcg gctttaccgg tgccaggctc ggcggctcgc ataaccatga tcctgaacga
     5101 aaatgaacaa cgaacagtgg acacgaggcg agagtggttc agactgctcg acgaaaaaag
     5161 tccgagacca atcccggccc catggttagc aattccatcg cgaagagcgc aaaggacacg
     5221 aaaagagagg gtgagaccgg atgagaatct gccataccgt ccttggtgtc accggccagg
     5281 gcgccggcgg gccacggcgc tcgagccgct gcagcctcga cactcttgat cacccaggag
     5341 tgcgcggacc gcgtcacatg ccacgctgcc tcgctgcttc gtgtccttcg tgcccttcgt
     5401 ggtgaagaca aagacaggcc cgatcgacat cgagagccgc aacatgattc gaggaatcag
     5461 tcggcagggg gcgctgaggg cggggtcgag ttgtcctcga cggcgtcttc cttatcttcg
     5521 ctgatcacgg gcgcgatgcc ttgcaggcgg tcactttcgt cgaggtcaat cagtttgact
     5581 ccctgcgcat tgcgtccggt ctcacggatg tccctggcgg cgatccgcac catctgaccg
     5641 ccggcggtga tcagcatgac ctcgtcgact tcgcggacgg tgagtgcgcc caggacgccg
     5701 ccggtgcgtt cggtggtctt catggtgatg atgcccttgc cgccgcgcga ctggatgcgg
     5761 tactcctcga agcgggtgcg cttgccaagg ccgttctcgc ccgcgacgag caaggtcgct
     5821 tccggatcca ccacggcggc cgccacaacg cggtcctcgg gatcgaggga gatgccgcgg
     5881 acgccggtcg cgggccggcc catcgagcgg acgtcctcct cggagaaacg gatgctcatg
     5941 ccgccgcggg tgatcagcac gacctcgtcg gtgccgcggg tgtagcggac atcgatgagc
     6001 gtgtcgccgg gctcgatgcc gatggcgatg atgccgccgc gacgcacgtt ggcgaactcg
     6061 gccagggcgg tcttcttgac ggtgcccgat tgggtggcga agaagatgta accgggctgc
     6121 tgccacgtga catcctcctt ggccgatccg gtcttgctct cgatgcggat gagcgcggtc
     6181 accttctcgc ccgcctggag ttcgaggagg ttggcgatgc tgcgtccttt ggcggcgcgg
     6241 cccatgtcgg ggatttcgtg cacgcgctcg acgtagaccc gccccatgtt ggtgaagaac
     6301 atgaggtagt cgtgcgtgct ggcggtgaag agccgctcga tgaaatcggg cgcgtcgccc
     6361 tcgacggccg agttctcgcg cgccgtcatg ccgatcaccc ccttgccccc gcggcgctgg
     6421 gcgcggtagg aggagacagc ggtgcgcttg atgaggctgt tgtgggtcat cgtgatgatg
     6481 acaccctcgt tggcgatcag atcctcgatg gcaatctcgc cctcgtcggg caccagatcc
     6541 gtcaggcgag gcgtggcatt cttccccttc acagcctgca actcggcctt gatgatggcg
     6601 aacacgcgag attccttggc caggatgtcg agcagatcgt ggatggtttc gagcaacgcc
     6661 ttgtactcgg ccgctaccct gtcgatctcg agtccggtca gttgatagag gcgcaggttg
     6721 agaatctcgt ccacctggcg ctccgtgagg ctgtatcggc cgttgaccag cagggcttcc
     6781 tggcgaatcc ggattcccca gctttcgacc tggagccggg tccactcgaa ggcgagcagt
     6841 ttgaccttgg cctcgtcgcg ggttctcgag gtgcggatga tccggatgaa ctcgtcgaga
     6901 ttggcgattg cggccaggta accttcgagc acctcggccc gctcctcggc cttgccgagg
     6961 aggtaccgag tccggcgcag gatcacctcg cgacgatgct cgatgtagca attgatcagt
     7021 tccttgagat tgagcgtctt gggccggcca tggtcgatgg cgagggcatt gaccgcgaaa
     7081 ctcgtttcga gcgccgtgtg gcggtagagg ttgttgatca ccaccttcgc cacggcatcc
     7141 cgcttcaact cgatcaccac ccgcgtgttc tcgtccgact cgtcgcggac ggccgtgatg
     7201 tctttgaact ccttgagttc gccttcgtta acaagcgtcg cgatccgctc gaccagttgg
     7261 gcccgattga cgttgtatgg gatttccgtg acgataatct gctcgcgtcc gcccttgagc
     7321 tgctcgaccc cgacccgccc gcgaatcttg acgctgcccc ggccggtgcc gaagtactgg
     7381 cggatgcctt cgagcccgca caccatgcag ccggtcggga agtccgggcc cttgatgtgc
     7441 ttcatcaact ggggaatggt gatgcccggg ttatcgatct gggcgcaaat tgcatcgacc
     7501 acttcgccca ggttgtgcgg ggccatgttg gtcgccatgc ccacggcgat gccagtgccg
     7561 ccgttgacca gcaggttcgg gaatgccgcg ggcagcacca acggttcctc gtgttcctcg
     7621 tcgtaggtcg gcgcgaaatc caccgtctgc ttgtcgatat cctccatcaa caccgcgccg
     7681 aggtgcgtca tgcgggcctc cgtgtaacgc attgccgccg gcgggtcgtt ttcgacggac
     7741 ccgaagttcc cctgtccatc gaccagcgtg tcccgcatcg accacggctg cgccatgtgc
     7801 acgagcgtcg ggtagatggc ctgatcgccg tgcggatggt atttgcccat cgtttcgccg
     7861 acgatgcggg cgcatttgag gtggcggcgc gtgggcagca ggcccaagtc gtggaacatc
     7921 gcgaagagga tgcggcgctg ggacggcttg agtccgtcgc gcacgtcggg cagggcgcgc
     7981 gagacgatca ccgacatcga gtaatcgagg aacgagttct tgatctcctc ggcgacgttg
     8041 atcttctcga ccttttcgtt cgcggcgtag agcggcgtgg ggctctcggg cggagggggg
     8101 gggggcggcg tttggttctc ttcgggcata ggagacgagg ggggcggaac gcttcagacg
     8161 tcgaggttgc gcacattgag cgcgttgtct tcgatgaact ggcggcgggg ctcgacttcg
     8221 tcgcccatca atttggtgaa catctcctcc gcctcgacgg cgtcggtcag gtcgatgcgc
     8281 agcagcttcc ggcgcgccgg gttcatggtc gtttcgaaga gttccttcgg gttcatctcg
     8341 cccaggccct tgaaccgctt gatctggaga ccccgtttcc cgacctcctt gaccccgagc
     8401 agaatctccg ggatggagaa gatcggcttg acttgggtcc gctcgccctc gccttccacc
     8461 agttcgaaga gcggtttgtc ctgggcggcg aagtgctcga cgttgagccc tttgcgggcc
     8521 agcttcgcga gcaggtcttc gacggccttg gtttcgtgca actccacgtg gcgcgcccgg
     8581 cgtgttgggc cgttgtggcc gtttttcctg tcggcgccgg ccgcggtctc ttcttcgccg
     8641 aagagcccga gatcggggtt ggcctcgccg aactcgcgca actcctcctc ggtgtggaag
     8701 tagtgcaccg cctcgtcgtt cccatgccgc actttcacca ggtggcgcgg cagttcgtgg
     8761 gtgtccaaac gccgtttctc gacatactcg gcgaagtcgc ctccgtggcg ccgcaacgcg
     8821 cgcacgtact tctcgaggac cacaagcaat tcgagaatct cggacaactg ccgttcgttg
     8881 aactcccgcc catcggccac gttgcgcagc cgcacgtctt cggagcccaa ctggatgagg
     8941 atgcgattga gttgcgggtc gtcctcgacg tattccaccc gcttcttgcg cgcgatctgg
     9001 cagagcggcg gctgggcgat gtagacgaac ccgcgccgga tcaactcggg catctgccgg
     9061 tagaagaacg tcagcagcag cgtgcggata tgcgcgccgt cgacgtcggc gtcggtcatg
     9121 atgatgatct tgtggtagcg cagcttctcg aggttgaagg ccccttcgcc ttcgctgtcg
     9181 ccgatgccgg tccccaccgc tgtgatcatt gttcggatct cgaggttctg gagcaccttg
     9241 tccaatcgcg ccttctcgac attgatcagc ttgccccgga tcggcaggat ggcctggaac
     9301 ttccgatccc ggccctgctt ggccgagccg ccggcggaat cgccctcgac aatgtaaagc
     9361 tcggtgttcg ccgggtcgcg gtcggagcaa tcggccagtt tgcccggcag gccgcccccg
     9421 gtcagcgcgg tcttgcgcac cgcttcccgc gccttgcgcg ccgcctcgcg cgcccgggcc
     9481 gccatcaggc acttctcgat gattcttttg gccaccgacg gattggcgtc gaagtaggtc
     9541 atcaagccgt catacacgat cgaggagacg acgccgtcga tctcggtgtt gaccagcttg
     9601 accttggtct gggactcgaa gcgggggttg ggcagtttga cgctcagcac gcagaccagt
     9661 ccttcccgca catcgtcgcc gctgatcgag gcgtctttct ccttcagcaa ttcattcgtc
     9721 ttggcgtact ggttgatggc acgggtcaat gcgctccgga atcccgtcag gtgcgtcccg
     9781 ccatcgggat tggcgatcga gttggcgaag cagagaatct gttcgttgta actgtcggtg
     9841 tactgcagca cgcagtccac aaacgcatcc tcctcgcgag tctcgacctt gatgacgcgc
     9901 ttgcccgaca ggcagaccgg cttgggatgg atcgtctgct tgctcttgcc caactgcttc
     9961 acgaactcct cgatcccgtc ccggtaaaag aatcgctccg acttgttctc cgtccgctcg
    10021 tcggtcagga ggatctcaac gcccgggttg aggaatgcca actcgcgcag ccggagcgcc
    10081 aggtgctcga acttgaactc cgtggtcagg gtgaaaatga ccgggtcggg cttgaaggtg
    10141 atcagcgtgc cggtgtgctt ggacttgccg atgacttcga gtttgcgcac ggtaacaccg
    10201 cgggcgaact ccatgtagta gaccttcccg tcgcgcgaga cctcgacctt gaaccattcg
    10261 gacagggcgt tgacacactt ggccccgacg ccgtgcagac ctcccgagta cttgtaagcc
    10321 ccctgaccga acttgccccc ggcgtgcagg ttggtcagca ccaactcgac cgccggcatg
    10381 ttccacttcg ggtgggtatc gaccgggatg ccccggccat cgtcgcgaat cgagcacgat
    10441 ccgtccacgt gcatcgtcac ctcgatgcgt tggcagaagc cagccaggtg ctcgtcaatc
    10501 gagttgtcca acacctcgaa cacacagtga tgaagcccgc gctcgtcggg gtcgccaatg
    10561 tacatgccgg ggcgcttccg gaccgcctcg agcccctcga gtttgtcgat tttggaggcg
    10621 tcgtacttgt cagtcgtcgg gacgttgtca gccatatttg aacctgctcg gactcgaaga
    10681 accagaccgc gaaaaaccgg atgttcaagc aacctgtctt cagggatacc agacggtgtc
    10741 gagtgtatga aaaccgcctc caaaaacaac accaaatctc gcgaatctac aatccgttgt
    10801 ggacgcgtcc gaactccgtc ctaattagtt gtggtcctcg atcccatcag aatcgttgcg
    10861 tatccccgcc cgagggcgtc agaagccggg ccaacgcctc gaatctctcc agacccagag
    10921 actcggctcg gcatgtctgg ctcaggtcca aggtctcgaa ggcgccgcgc aacgcctctt
    10981 catcccagcc ggatctgagc agcttgaaca tcatcttcct gcgctgggag aaagcgctcc
    11041 gaaccaacct tgcgaatgtg cgccgttcga acgcggagaa accaggtccc gatctccgat
    11101 ccagagccag gcaggtggtg gcgatcttgg gaggggggaa gaaacaactg gccgggatgc
    11161 tgaaggaagc ggccggcgca tacctgtgtc ggacgatcaa ggtcaggatg ccatagtccg
    11221 gcgatccggg tccgacggac aggcgttggg cgacctcctg ttgcagggtg atgacgagcc
    11281 gctccggaca tgtcggggcc agggcgaggg tcatcaggat ttccgacgcg gccgagtacg
    11341 gaagggcagc gacaagcttc cacgaggccc aatcctcggc atgcccggcg aggaaatcaa
    11401 cggcatccgc atgcaccagg cgcagtctcc ggtctccggc aaaccgttca tcgagacaac
    11461 ggaccagacg cgcatccttc tcgatggtca gcacctcaca cgccccggcc aggagcagct
    11521 cggtcaatgg cccgaggccc gggccgatct cgatcacctg gtcgttcggt cgcaggccgg
    11581 ccaggcccac catccggcgg agctggttgc cgtcgtgcag gaagttctga ccgagcgatt
    11641 tggtgaggcg aatgcgccgg gcggtcagaa tctggcgcat ttcggagaga gtcatgccga
    11701 cccgagttgt gccagggcgt cctcgagcgc tccgacggcc cgttcgagat cgatttggct
    11761 gatgacgagc ggcggcgtga atgcaaggac gttcccatgt tctccttcgg gcaaaatgag
    11821 gaccccccga tcgagcatga ccctgacaat ctcgcacaca gcgtcgcagg ccgggcgacc
    11881 gtcgcgatgg cacagctcga agccgaccat caaaccgagc ccgcggacat cgccgaccag
    11941 gccggcgggc accgccatcc ggctcaaccg ggtgcgcagg accgccccca gacgctcgct
    12001 tcgccgcgga aggcgtctgg atcgaatctc gccgatctgg ctcagggcca tggcgcatcc
    12061 gaccgggtgt cccaggaacg tgcttgtgtg gatggcttcg cccgtcgcct ccggccatgc
    12121 gcggtccatc acatccgccc gaccgacaca cgccgacagg ggaaagccgc cggtcaatgc
    12181 cttccccaaa cagaccaggt ccggaacaac gccgccccgc tcgcaggcga accagcgccc
    12241 ggtgcgtccg aatcctgtgt agatttcgtc cacgatcagg agcgcaccga actggtcgca
    12301 taacgctcgc acgcgcgaca ggaagtccga gggcggcacg cggatgcccg accgtccctg
    12361 cacaggctcg atcaggacgg ctccaatccg gcccgaaccc aggtgatggc gcacggcggc
    12421 acaggcgcgc gcagcggcgt cgtcggaaac agggaaaggc gcaaaccgca caaacggatt
    12481 gagctgggac cgaaacggca gccggaaatg cgcgcgctcc gtggccgtga gcgcgccgta
    12541 cccgagcccg tggtaggcgc cttgaaaggc gacgacgccc gccctgccag tggcgagcag
    12601 cgctgtcttc agtgcggcct cgacagcctc gaagccggaa ttcccgaaga tcactttgcc
    12661 ggtccggggc ggttgacgag tcgcgccgat catcgtgcgt ccgcgatccg aaatccgcga
    12721 ttcccctgca ccgcccgtcc aacgctcgaa cgtgaggcgg ctcaactcgc gagccagcgt
    12781 ggccttgaga cgatgcggat acacatcgcc catggcgtgc aagagatgtg acatttgccg
    12841 ccgggcggtc gcgacgaccc gcgggttggc gtggccggcg gccgccacgc cgaacgccgc
    12901 ggtgagatcg aggtatcggc ggccttcgag atcccagacg tgcgccccgc gggcgcgctc
    12961 ccagacgatc ggcccggaga ggtccggagc gatgagcgtc acgttgcgcg attcgtaggc
    13021 gcgcaacaga tcgagaatcc gacgggtggc cttcactcaa acgcctcgtt gatcggcggg
    13081 ccagatgacc ttgtgcaatt gcacctggaa acggaccggc agcgcgtccg caatcacccg
    13141 ctcggccagg gcgcgccgcg agatcggatt ctggccggga gggatggact tgagagatgg
    13201 atcgcgctgc cggggctcga ccggcgaaac ccaggagaac aggatcgggc acatctcatc
    13261 gaggcggcgc tcgaagacga cctcccgcgc ccagcgatag tcttgttcgg ttccgatcac
    13321 gaacttcact tcgtcggtcg cccgcaggtg cgtcaggttc tcccatcgat tttgggcgct
    13381 ctcgccgctg ctcgggcact tgatatccat aacgcgccgc acgcgcgggt cgaccggcgc
    13441 gatgtcgagc gcgccgctgg tttccagcag cacggtgtaa ccgtcgtcgc acaacgcttc
    13501 gagcagcggc agcgatcccg gctgcagaag cggttcgccg cccgttaact cgaccaaggg
    13561 aatcgagacc ggccggcgaa cggcgcgata gggcgccgcc aaacggcgga cctcggcccg
    13621 gatgtcctcg agcgtccgac ggcgtccttg cctgaacgca taggcggtgt cgcaatacga
    13681 gcagcgcaga ttgcatcccg tgagccggat caggacgcac ggcagaccgg cgaatgtgct
    13741 ttcgccctgt acgctgaggt agatctcgtt gacggtcaga tgatcgggca tgcgggccag
    13801 catggttgtt tcacaaagct ctgcggagtc cgacacccgg gctctggcgc ctcagacaaa
    13861 tccagtcctc cccttccgat gaggtgtccc cgtgtctgtc cgcctttctc tcccgcgcct
    13921 cgctcattag aatccctgac ccgttcaacg cagcacggaa actcgacttg tctgcaacat
    13981 gtttcaccgg tcaaggattc taggcggttg gagccggttt ctgatcggcc acggcgccgg
    14041 cttcgaggag tcgcgggcct cgttcgcgca agggaaacca gaggcgaaac acggtgccct
    14101 tgcccggatg gctttcgaca tcgatgcgcc cgccgtgctc gcgcacgatg cggtagacga
    14161 tcatcatgcc gaggccggtg ccctttttct tcgtcgtgaa gaacggctcg aacagccggc
    14221 tgagccgctc ctgcgaaatg cccgtcccgg tatcgcccac gctgatccag acgccctcgg
    14281 acgcggtgcc tgtccgaatg gtaagaacac cgccccgggt catggcgtgc atcgagttct
    14341 tgatgaggtt caccagggcc tgtttgatct gcgcggcgtc aaatcgtgcc tcgggcagat
    14401 cgcgcgccag gtgcgtctcg acggcgagac cgcggttgtc gagttccggg cggagcaagt
    14461 cgagtgtcgc cttggccacc tcgttcagca acgcgggccg catctgggga accgtcgggc
    14521 gaatcgcatc gagaaactgc gtgacgatgt agtccagacg cacgatctcg ccctgggcca
    14581 ccgtgaggta cttgcgcatc cggtcggcaa tttccttcgc ctcgtccccg gagggcgagg
    14641 cggacgatgc ggcgcgccgc cgtcccgggc gcgagggggc tga
//
LOCUS       DAOQRO010000030        14553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3211_length_14553_cov_5.078632, whole genome shotgun sequence.
ACCESSION   DAOQRO010000030 DAOQRO010000000
VERSION     DAOQRO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14553
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_14553_cov_5.078632"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1878)
                     /locus_tag="PKK20_02195"
     CDS             complement(202..1878)
                     /locus_tag="PKK20_02195"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNU98731.1"
                     /translation="MKTTRREFLGTSAGIVGSVALGSAGTTWANDSAEDAPTRLFTYH
                     GLRPTDPGGRLGLRNPERGWRIETVIAEPARKTFGPAHHLIGRVAPIYEADWWMLDAA
                     RFEPFGLTLVQAYCYLTEFADRPISNEKLALLQESLDNLRHRGLKAVLRFAYERDMGV
                     KEGPTPDWILRHLDQLEPMLRKNVDVIYVLQAGFVGAWGEWHSAARIAPDDYAARAAI
                     IKRLLEALPEGRMLQVRVPKYKRLALQQPALAAFVEVGPQSAFTNVPAARIGFHDDGF
                     LAGPSDGGTWPEPPHFGQPGNAEFDYMTRESAFVPVDGELFWTDQGFDGKAARGKGVD
                     GLNAAVRMRLHHYSSFSLAHSYSEREGRPYSIDHWLSTAIVPESLRQAAMPVGDGWFE
                     DGFGQPAMRTQFEYIQDHLGYRLELQSARIPEKVKTSAPFRVEIELINRGFSTLHNPR
                     PVCLALMDRTGKVTELSVPDADPRQWQPFRPGDGEFKPIVHRIAWRGTLPAGIPTGWQ
                     QVGLWLPDAAESLRRDPRYAVRVANRDAPWWTDVRGQYGINVLGVVEVVP"
     gene            2280..2365
                     /locus_tag="PKK20_02200"
     tRNA            2280..2365
                     /locus_tag="PKK20_02200"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2314..2316,aa:Tyr,seq:gta)
     gene            complement(2390..3271)
                     /locus_tag="PKK20_02205"
     CDS             complement(2390..3271)
                     /locus_tag="PKK20_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNU98732.1"
                     /translation="MQIESLKVFCDLTETESFTKAAQINHITQSAVSQQISSLERQFK
                     SLLIERSKKKFRLTREGRVLYEASKQIVQTYESLYSRLQELKDVISGTIRIATIYSIG
                     LHDLPPYIKRYLKSYPTVNVHVEYRRANQVYEDVLGNIVDLGLVAYPTRDSKLEIVLL
                     KKEPMVLICHTQHPLARTKSVKIDALAGQKFIAFEPDIPTRKAIDKVLREHGVHVHPV
                     MEFDNVETVKRAVEIDAGIAIVPLSTVTLEVQQKTLAAVTIDDAELHRPIAAIHKKSK
                     VLSLAMKHFLAMLRQTE"
     gene            3320..3886
                     /locus_tag="PKK20_02210"
     CDS             3320..3886
                     /locus_tag="PKK20_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98733.1"
                     /translation="MHSDICFPYPISESRGGAHGLNGVRPAPTLSRLGKLASRLGLLL
                     ACLSAQVGCQSPAASRSDALVSMTIVSPHPTRVRLAVVKVFEAEHFEGKSVFDPEMVF
                     ERRGSLGDSLLRGGLLSGKTIERVRVRVTSVGPETYQLDCRAFTVQYPGDRVMEEEFR
                     IRRKGPYEELLERVRAQVAKLREAPDLP"
     gene            3940..4680
                     /locus_tag="PKK20_02215"
     CDS             3940..4680
                     /locus_tag="PKK20_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNU98734.1"
                     /translation="MFEAADLFDLSQTEHAEVFAKCAYGWDALKGLAAYIDAHVRPAL
                     RNRCEGVAYIGERVFIGAGTVVEDGVMIKGPAIIGRNCQIRHNAYLRENVVVGDDCVV
                     GNACELKNALLFNRAHVSHFNYVGDSILGYRSHLGAGVKISNVKIFPGHVMVEVEGKR
                     IDTGLAKFGALVGDRAEVGCNAVLNPGTILGRDTRVYPNVNWRGALAAGGIAKNRAQV
                     EVIVRSPSDPGLSDASGATPSGLGTDAA"
     gene            4754..6886
                     /locus_tag="PKK20_02220"
     CDS             4754..6886
                     /locus_tag="PKK20_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNU98735.1"
                     /translation="MISTVENIPAGATDLVERVHRLFSPKGILAKARNFEYRPQQQQM
                     ATAVARALAYGEHLVVEAGTGVGKSLAYLVPAIFHAVEQRKKAVISTHTINLQEQLTQ
                     KDLPLLERVLPVRFKFTMLKGRQNYLCTRRLEKAMQQSDSLFTTPETQELQRIYEWSK
                     TTEDGSLSDFENEPDPRVWAQVCSERGVCSPKKCGTDSDFAKAGGAPCFFQRVRSQIM
                     SADVLVLNHTLFFMHLGGIEEDTGDTGVLFKNDFVIFDEAHTVEEVAARHIGLSVSNA
                     QLRFNLQRLWNERTGKGLLSTLRQGAAVNLVADAYEQSSKFFDAVEGACDALHHRTRK
                     RGPGFIPPVTAELASPAPGRAWSELRIRRPDLVADSLTLPLARVREAVSEMVKLSKDS
                     DMGTELIECNRRLGELREAIATFLSQSADGHVYWVERSGRGQKQLALNAAPVDIADFL
                     RRRLFACDTSVILTSATLGMDVKRLGEPVRAEPGRKDGLNYFVNRVGADEATLLQVGS
                     PFDYAEQMRLYVVGRMPDPRDAAYRAALVRWIGHFIRQTHGKAFVLFTNSKLMQEIGE
                     EMAPSMDELGVSCFVQGTGTPRSVMLDKFKKDVDSVLFGTASFWQGVDVPGESLSNVI
                     ITRLPFAVPDHPLTEARIESIEAGGGNAFMDFSLPEAILKFRQGVGRLIRTKTDRGIV
                     VVLDNRILTKRYGKAFLDALPKCPVEVV"
     gene            complement(6979..7779)
                     /gene="thiD"
                     /locus_tag="PKK20_02225"
     CDS             complement(6979..7779)
                     /gene="thiD"
                     /locus_tag="PKK20_02225"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNU98736.1"
                     /translation="MTTRSSQPVALTIAGSDSGGGAGVQVDLKTFAAFRVHGTSAITC
                     LTAQNPRAVLGIHPTPPGFLRQQLEALFAELPPSAVKTGMLFSRANIRVAIDWFRAPG
                     RPPLVVDPVMVASSGARLLRPAAIKALIEGLLPLARLVTPNLDEAQILVGRPLRSIED
                     LRSAAREIHQAYGCAALVKGGHLRQMREAVDIFYDGREELLLTAPFVRGVTTHGTGCT
                     YSAAITACLAMGHPLNKSVRRAKEHITGAIAHSIRIGRHSTLDAFWRR"
     gene            complement(7881..10727)
                     /locus_tag="PKK20_02230"
     CDS             complement(7881..10727)
                     /locus_tag="PKK20_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNU98737.1"
                     /translation="MIESFVPDRLGRAFLATALFGAPFFLHAIADASDAPPASSPAAR
                     LIAQRGPLVIAHRGFSQQAPENTLPAFRLALAARADLVELDYYHAKDGVPVVIHDGTL
                     DRTTDATNRWGGKGIAVTSRNASELTSLDAGRWFSPAYAGTRLPTLVESIETIQAGGV
                     TLIERKGGDAPALVRLLRERGWLDSLVVQSFDWGFVRDARRVAPDLVLGALGPPSSRN
                     GRKLTDQEKVLGPEFIADVEQLGAQLVVWNRQVTADAIAHAHQRGLRVWVYTINDPNE
                     AAQLVRLGVDGIITDNPAVIRARLHPQADESADAPSTRAIPKPLPEHPGNIYLEGETV
                     QVRLPEGLPDTATGWRLLDDQARVLRSGALSGEAVQARTPIAAGDLDIGWYRIEFDAP
                     ERTNILWTTAAVIRRLVAPVPGDSPVCVDSATAWFARNDPPRQQRFANLAALAGVNWV
                     RDRLRWSDIQPEPGALVPAGTTYDTAAEAQHAAGLKVLQVFHDTPSWAREESSAGGRF
                     APDLRHAFDLGRALAARFKGRVAAWEPWNEANVSTFGAHTVDQMCSWQKAAWLGFKAG
                     DPGVIVGWNVTTAAPTPAQTAGVLANETWPYFDTYNIHTYDWPHSYAQLWKPAREAMS
                     GRPLWVTEADRGTPHLKQGPWYDQDSRLERLKAEWLAQSYASSLFAGTRRHFHFILGH
                     YHEPNGVQFGLLRLDMTPRPAYVALAAVGRCLAGARALGRFQPGGDAHVYAFRAKPDG
                     QERDVLVAWAEKEIEWDGRGHTASAWKPPVNLDVQGVVDYLGRPAGKALPERLTSAPV
                     FVFLPAGQAATLPLEPPPPLATPRPGTVSPVVLQLSLPRSATARVQDIPWSEGYVYSV
                     KPGESLNLPIHAYNFGTARAEGRIQIERQPEGWEVQVPSTEMNVGPRDRVALEARLRV
                     PAGAACRDGWVVLRADCGDQGRPVLAFRVLAKDE"
     gene            complement(10864..11364)
                     /locus_tag="PKK20_02235"
     CDS             complement(10864..11364)
                     /locus_tag="PKK20_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU98738.1"
                     /translation="MGQRAGVGLKCASVDNGRRVLELGPGTGVVTEALLARGLDPRRL
                     IAVEKSPCLIEVLRERFPEARFVPGDALELDALFPGERFGAVVSSLPLNNFAAPNVAR
                     LAACIRAILDPGGCWIQFTYRVLNGQPPSDAFEQVDSELVLLNLPPALVCVYRPVTLD
                     PARPPL"
     gene            complement(11698..12972)
                     /gene="ribD"
                     /locus_tag="PKK20_02240"
     CDS             complement(11698..12972)
                     /gene="ribD"
                     /locus_tag="PKK20_02240"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNU98739.1"
                     /translation="MRLAAAPNDIRFMRLALRLALRGYGQTSPNPMVGAVLVKNGRVI
                     GQGWHHRAGQPHAEIEALGDAARRGCSVRGATLYVTLEPCSTAGRTPPCTEAITRSDI
                     ARVVAAATDPNPNHAGRGFQVLRQAGVKVEHGLLAEEAARLNESFNHWIVERLPFVTV
                     KAAMTLDGKIAAATGESKWITGAIARRHAMRLRLGADAILVGVNTVLADDPSLTARGA
                     TRPGYGPEADAGIRSVATLQTGVGARTCSTPVKPIRRLVLDARARTPLDCRLITDPWA
                     AWTTVYVGPTAPVRRRDALARRVRVVVAPEIEQRGGVRGSRHRGARQSACRPKLNLDW
                     ILRQLGREEVTAMLIEGGGELHASLFESGLVQRVAFYYAPKVLGEAAGRKAVAGAGFG
                     DWPTVVSLRDIEWDRVGTDLLLTARVGPAQEK"
     gene            13054..13413
                     /gene="cutA"
                     /locus_tag="PKK20_02245"
     CDS             13054..13413
                     /gene="cutA"
                     /locus_tag="PKK20_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNU98740.1"
                     /translation="MKKTRGFHMVVVTAPDMKTARRLARVALEARLIACASLIPRIES
                     HYWWKDRIESGSEVLLLMKSADRSLSALERRIMAEHPYDTPEFLVCPIAGGNARYLAW
                     LEANVVSSRSSERRRRT"
     gene            complement(13442..14311)
                     /locus_tag="PKK20_02250"
     CDS             complement(13442..14311)
                     /locus_tag="PKK20_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNU98741.1"
                     /translation="MPHTWPNGRTLLSLARLPGLITVWSNCLAGWLLSGGDTHSPARL
                     LWCSLGASCLYLGGTFLNDFFDVDFDRQQRPNRPIPAGLVARDVVGQLGILWLIAGGA
                     ILVLGARVGLTLALMLAVAVFAYNLIHKATELSPLLLGFCRFLLYLLAGATAPNGLAG
                     IAIWSGLAIGCYIAGLSWFARRQGIRGPIRRWPLVLLAVPLGLAWISNADGDRHVAVA
                     LGLLLCLWILPWLSHALREREPNIRMTVSGLTAGIVLVDLLAVGGHSTIVTLVFAALF
                     LTVLLAQRYLPES"
     gene            complement(14311..>14553)
                     /locus_tag="PKK20_02255"
     CDS             complement(14311..>14553)
                     /locus_tag="PKK20_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98742.1"
                     /translation="KPPQVREKEDRNTLFLGRNAFTTGRMLHIPFLSAVLPQLGNPRP
                     RPCLRFPGLGLLFSRVLFCYPPLGTGNPPDQPRERH"
BASE COUNT         2612 a         4542 c         4895 g         2504 t
ORIGIN      
        1 tcaattcctg ctgccgatgg ccctggcggg gggcggctca ttacgcatgg tcgcgccgtc
       61 atcgcacgcc acgaccaacg ccacgatcat cgagaggttc ctgccattgg ccataaggtt
      121 cgagcgggag aatgagttgc catggcgcgc cacggtcggc gcccgctgac ttgccctgca
      181 agcgtcgaag tcctgcgtcg atcacggaac cacttcgacg acccccaaca cgttgatgcc
      241 gtattggccc cgcacgtcgg tccaccaggg cgcatcgcgg ttggccacgc gcacggcgta
      301 gcgaggatcg cggcgtaacg attcggcggc gtctggcagc cacaaaccga cttgctgcca
      361 gcccgtcggg atgcccgccg gcagtgtgcc gcgccaggcg atgcgatgga cgatcggctt
      421 gaattcgccg tcgcccggac ggaagggctg ccactgccgc ggatcggcgt caggcacgga
      481 caactcggtt actttgccgg tgcggtccat cagcgcgagg cagaccggtc ggggattgtg
      541 cagtgtggag aaaccgcggt tgatcagttc gatctccaca cggaacggcg cgcttgtctt
      601 caccttctcc gggatgcgcg cgctctgcag ctccagccgg tagccgagat gatcctgaat
      661 gtactcgaac tgcgtacgca tcgccggctg gccgaagccg tcttcgaacc acccatcgcc
      721 cacgggcatg gcggcctgcc gcaacgactc cggcacgatg gcggtgctca accaatggtc
      781 aatcgagtac ggccggcctt cgcgctccga atagctgtgc gccaggctga agctgctgta
      841 gtggtgcagg cgcatccgca ccgcggcgtt gagtccgtcc acgcccttgc cgcgagcggc
      901 tttgccatca aagccctgat cggtccaaaa caactcgccg tccaccggca cgaacgcgct
      961 ttcgcgggtc atgtagtcga actctgcgtt accaggttgc ccgaaatgcg gcggttccgg
     1021 ccacgtcccc ccgtcggaag gaccggccag gaagccgtcg tcgtggaatc cgatccgtgc
     1081 cgcgggcacg ttggtgaagg cactctgcgg gccgacctcc acgaatgcag ccaacgccgg
     1141 ctgttgcagc gccagccgtt tgtacttcgg gacccgtact tgcagcatcc gcccctccgg
     1201 cagggcctcc aacagccgtt tgatgatggc ggcgcgcgcc gcatagtcgt ccggggcgat
     1261 ccgggcggcg ctgtgccact cgccccaggc gccaacgaac ccggcctgga gcacgtagat
     1321 cacatcgacg ttcttgcgta acatcggttc gagctggtca aggtgtcgca gaatccagtc
     1381 cggcgtgggg ccttccttga cccccatgtc gcgttcgtag gcgaaccgca gcacggcctt
     1441 caaaccacga tgccgaaggt tgtctaggct ctcctgtagc agggccagct tctcgttcga
     1501 aatcggcctg tccgcgaact cggtgaggta acagtaggct tggaccagcg tcagcccgaa
     1561 gggctcgaag cgcgccgcat cgagcatcca ccagtccgct tcatagatcg gcgctacgcg
     1621 accgatgagg tgatgggcgg gcccgaaagt cttccgggcc ggctcggcga tcacggtctc
     1681 gatccgccaa ccacgttccg gattgcgcag cccgagccgc cctcccggat cggtcggacg
     1741 aagcccgtga taggtgaaga ggcgcgttgg tgcgtcttcg gcgctgtcat tggcccaagt
     1801 cgtgccggcg gagccgagcg ctaccgaccc gacgatgccg gctgaggtcc ccagaaactc
     1861 gcgtcgtgtg gtcttcatat ttatgggaaa ggagcctaac acgcgccggt cccaggcgcg
     1921 agagcttcag ctctacacgg catcgcgggg cgggctaata ctgagaaagt cgacgagcct
     1981 cgccttggca gtcgagaaaa cactggttcc gaggctttca cgaatgggct tgcctccgac
     2041 tttgacgcgg gcataatacg tcgcgctggt cacgtactga agcaagtgcg gtactttcgg
     2101 gaatgacctc cacttgccgt ctttggacgg tgaggaggag tttttccggg ggtggattcc
     2161 ggtggttacc ttaggcgcag tggccatacg aggcacgctg cgagggatac aagaccggga
     2221 tacaaagaaa agatcgctac ttgtggaagt tgctgatggc gaatggtttg gtaacggtgg
     2281 gtgggatacc caagcggcca acgggggcag actgtaaatc tgctggctta tgcctacact
     2341 ggttcgaatc cagttcccac caccacccga agacgctcgg tcgcgtctct cactccgttt
     2401 gcctcagcat cgcgaggaag tgtttcatcg ccagggacaa gaccttggat ttcttgtgga
     2461 tcgcggcgat ggggcgatgg agttcggcat cgtcgatggt cacggcggcg agggttttct
     2521 gctgcacctc cagggtcacc gtgctcagcg gcacgatggc aatgccggcg tcgatctcga
     2581 cggcgcgttt gacggtttcg acgttgtcga attccatgac cgggtgcacg tgcacgccat
     2641 gttcgcgaag gaccttgtcg atcgccttgc gggttggaat gtccggctcg aacgcgatga
     2701 atttctgtcc ggcgagggcg tcgatcttga ccgacttagt cctggcaagg ggatgctggg
     2761 tgtgacagat caggaccatc ggctccttct tgagcagcac gatctccagc ttcgaatccc
     2821 gggtgggata ggccaccagg ccgaggtcga cgatgttgcc gaggacgtcc tcgtacacct
     2881 gattggcgcg acgatactcg acgtgcacgt tcaccgttgg atagctcttc aggtagcgct
     2941 tgatatatgg cggcaggtcg tgcagcccga tgctgtaaat ggtcgcaatt cgaatcgtcc
     3001 ccgagatgac atccttcaat tcctgcaacc ggctatacag cgattcgtac gtctgcacga
     3061 tctgtttgct ggcctcgtac aacacccgcc cctcgcgcgt gagtcgaaac ttcttcttgc
     3121 tccgctcgat cagcagcgac ttgaactggc gctcgagcga actgatctgc tggctgacgg
     3181 cagattgggt aatgtgattg atttgcgccg cttttgtgaa actctccgtc tccgtcaggt
     3241 cacagaagac tttcagactt tcgatttgca tgagcaaaga tgaagataaa gacttctgga
     3301 agtcaatact cttaatgcca tgcattccga tatttgcttc ccttatccga tctccgagag
     3361 ccgcggcggg gcccatggct tgaacggcgt ccgccctgct ccaacactgt cgcgcctggg
     3421 caagctcgcc agccggctcg gtttgctact ggcgtgcctc tcggcccagg tcggctgcca
     3481 gtcgccggcc gcctcgcgat ccgatgcgct ggtctcgatg acgatcgtat ctccccatcc
     3541 gacgcgggtg cggctggccg tcgtgaaggt tttcgaggcc gagcatttcg aggggaagtc
     3601 ggttttcgac cccgagatgg tgttcgagcg gcgggggagt ctgggcgaca gtctgctgcg
     3661 cggcggcttg ttgtcgggca agacgatcga gcgggtgcgg gtgcgcgtga cctcggtcgg
     3721 cccggagacg tatcaactcg actgcagggc gttcacggtc cagtacccag gggatcgcgt
     3781 catggaggaa gaatttcgga ttcgccgcaa ggggccgtac gaggaactgc tcgagcgggt
     3841 ccgggcccag gtggcaaaac ttcgggaagc gccggacctt ccgtgagcgc ggctgcggtc
     3901 ggcgcttgct caaggactgg cttccactac tctggcgtca tgttcgaagc ggcggatttg
     3961 tttgacctga gccagaccga gcatgctgag gtgtttgcga agtgtgccta tgggtgggac
     4021 gctttgaagg ggctcgctgc ttacattgac gcccatgtgc gtccggcact gcggaaccgg
     4081 tgcgagggcg tggcgtacat cggcgagcgg gttttcattg gcgcaggcac ggtggtcgag
     4141 gatggtgtca tgatcaaggg gcccgcgatc atcgggcgca actgccagat ccggcacaac
     4201 gcgtatctgc gcgagaatgt ggtcgtgggg gacgactgcg tcgtgggcaa cgcgtgcgaa
     4261 ctgaagaacg ccctgctttt caaccgcgcc catgtgtccc acttcaacta cgtgggcgac
     4321 tcgatcctgg ggtatcgcag tcatctgggc gcgggggtca agatctcgaa cgtgaagatt
     4381 ttcccgggcc acgtgatggt cgaggtggaa ggcaagcgca tcgacacggg cctggcgaag
     4441 ttcggggcgt tggtgggcga ccgcgcggag gtgggctgca atgcagtgtt gaaccctggg
     4501 accatcctgg gccgagacac gcgcgtgtac ccgaatgtga actggcgcgg cgccctggcg
     4561 gcgggtggca ttgcgaagaa ccgagcgcag gtcgaggtga ttgtgcggtc cccgtccgat
     4621 ccgggtctgt cggacgcctc gggagcgacc ccatccggat tgggaaccga cgccgcgtga
     4681 acgccgggcg atgcagtgtc gcgaatgcgg ttgagaatgc gggcggtatg gtcaacggcg
     4741 gcatgggagg aacatgatca gcacagtcga gaacatccct gcgggagcca cggacttggt
     4801 ggaacgggtg catcggttat tctcgcccaa gggcatcctg gccaaagcgc ggaacttcga
     4861 gtaccgcccg cagcaacagc agatggcgac ggccgtggcc cgcgcgctgg catacggcga
     4921 gcatctggtg gtcgaggccg gcaccggtgt cggcaagagc ctggcctacc tcgtcccggc
     4981 cattttccac gccgtcgagc aacgcaagaa ggcggtgatc tcgacacaca ccatcaacct
     5041 gcaagaacag ctcacacaga aggacctgcc gctgctcgaa cgcgtcctgc ccgtgcggtt
     5101 caaattcacg atgctcaaag ggcggcagaa ctacctgtgc acgcgccggc tcgagaaggc
     5161 gatgcagcaa tcggacagtc tgttcacgac gccggagact caggagctgc aacgcatcta
     5221 cgagtggtcg aagacaaccg aagatggcag cctgtcggac ttcgagaacg agcccgatcc
     5281 cagggtgtgg gcgcaggtct gctccgagcg cggcgtgtgc tcgcccaaga aatgcggaac
     5341 cgactcggat ttcgcgaagg ccgggggcgc tccctgcttt tttcagcgcg tgcgcagcca
     5401 gatcatgtcg gccgatgtgc tggtcttgaa ccacaccctg ttcttcatgc acttgggcgg
     5461 catcgaggag gacacgggcg acacgggtgt cctgttcaag aacgacttcg tgatcttcga
     5521 cgaggcacac accgtcgagg aggtcgcggc gcggcacatc ggcctcagcg tctcgaacgc
     5581 tcagcttcga ttcaatctcc agcgtctatg gaacgagcgg accggcaagg gtctgctgtc
     5641 cacgctgcgc cagggcgcgg cggtgaacct ggtggccgac gcctacgagc agagcagcaa
     5701 gtttttcgac gcggtcgagg gggcgtgtga tgcgctgcat caccggacgc ggaagcgcgg
     5761 gcccggtttc atcccgcccg tcacggccga gctcgcatct ccggcccccg gccgcgcctg
     5821 gtccgaactg cggattcgcc ggcccgatct ggtcgcggac agtctcacgt tgccgctcgc
     5881 ccgcgtccgc gaagcggtga gcgagatggt gaagctgtcc aaagactcgg acatggggac
     5941 ggagttgatc gagtgcaatc ggcggctggg ggagttgcgc gaggcgatcg ccaccttctt
     6001 gagccagagt gccgatggcc atgtgtactg ggtcgagcgg agcgggcgcg gacagaagca
     6061 actcgcgctc aacgcggcgc ctgtcgacat cgccgatttc ctgcggcgcc gtctcttcgc
     6121 gtgcgacacc tcggtgatct tgaccagcgc gacgctcggg atggacgtca agcgcctggg
     6181 cgagccggtc cgggccgagc ccggacgcaa ggacggcctg aattacttcg tgaaccgtgt
     6241 tggcgccgac gaggcgacat tgctccaggt tgggtcgccc ttcgattacg cggaacagat
     6301 gcgtctttac gtggtgggga ggatgcccga cccgcgcgat gcggcctatc gggcggcgct
     6361 ggtgcggtgg atcgggcact tcatccggca gacccatggc aaggccttcg ttctgttcac
     6421 caacagcaag ctgatgcagg agatcggcga ggagatggcg ccttccatgg acgaactcgg
     6481 cgtcagttgc ttcgtccagg gcaccggcac gccccgatcc gtcatgctcg acaagttcaa
     6541 gaaggacgtt gattcggtgc tgttcggcac cgccagtttc tggcagggcg tggacgtgcc
     6601 gggcgaatcg ctcagcaacg tcatcatcac ccgcctcccc ttcgccgtgc ccgatcatcc
     6661 gctgaccgag gcgcgcatcg agtcgatcga agccggtggc ggcaacgcgt tcatggactt
     6721 ctccctcccc gaggccatcc tcaaattccg gcagggcgtc ggacgcctga ttcggaccaa
     6781 gaccgatcgc ggcatcgtgg tggtgctgga caatcggatt ctgaccaagc gctacggcaa
     6841 ggcgtttctc gacgcgttgc ccaagtgccc ggtcgaggtc gtctaacccg catcttcaca
     6901 cttcgtcagt tcggaggacg ctcgggttgt gctggaggac tgccatgcag atgggaagag
     6961 gcggggacgc gaccgatctc aacgcctcca gaacgcgtcg agggtgctgt gccgtccgat
     7021 ccggatcgaa tgcgcgatcg cgcctgtgat gtgctccttc gcccggcgca cggacttgtt
     7081 caacggatgc cccatcgcca gacacgcggt gatcgccgcc gagtaggtgc accccgtccc
     7141 atgggtcgtg acaccgcgca caaacggggc agtcagcagc aactcttcgc gtccgtcata
     7201 gaagatgtcc accgcctcgc gcatctggcg caaatgcccg cctttgacca gcgcggcgca
     7261 gccgtaggcc tgatgaatct cgcgggcggc actgcgcaaa tcctcgatcg accggagcgg
     7321 tcggccgacc aggatttggg cctcgtcgag gttcggggtc acgagtcggg ctagcggcag
     7381 caggccctcg atcagcgcct tgatcgccgc gggacgcagc agcctggcgc cgctgctcgc
     7441 caccatgacc ggatcgacca ccagaggggg gcggccgggc gcgcgaaacc aatcgatcgc
     7501 cacccggata ttggcgcgcg agaagagcat gccggtcttg acggcagagg gcgggagttc
     7561 ggcgaagaga gcctcgagtt gttggcgcag gaaaccgggc ggcgtcgggt ggatccccaa
     7621 gacagcgcgc gggttctggg ctgtcaggca ggtgatggca cttgtgccgt gcactcgaaa
     7681 ggcggcgaag gtcttcaagt ccacctgaac gccggcgcca ccgccgctgt cggagccggc
     7741 aatggtgagc gccacgggct gactcgatcg ggtcgtcata agaacctctg tactttcaac
     7801 gcaacgcaga cattcgatct gtcggcaacg agtttcaccg gtcaacggtt ccatcgcgcg
     7861 agccgcgcca cgccgggcca tcactcgtcc ttggcaagga cgcgaaacgc caagaccggc
     7921 cggccctggt cgccacaatc ggcccgaagc acgacccaac cgtcacggca ggcggcgcct
     7981 gcaggcacgc gcagcctcgc ctcgagcgcg acgcgatcgc gcggcccgac gttcatttcg
     8041 gtcgatggca cttgcacctc ccagccttcc ggctggcgtt cgatctggat tcgtccttcg
     8101 gccctggccg tgccaaagtt gtaggcgtgg atggggaggt ttagggattc ccccggcttg
     8161 acggaataca cgtagccttc ggaccacggg atgtcctgga cgcgcgcggt tgcgctgcgc
     8221 ggcagagaca gttggagcac gacgggcgag acggtgccgg ggcgaggggt ggcaaggggg
     8281 ggcggaggtt cgagtggcag ggtcgccgct tgacccgcag gcaggaagac gaacactggc
     8341 gccgaagtga gtcgctcggg gagcgctttg ccggcgggac ggccgaggta atcgaccacg
     8401 ccctgcacgt cgaggttgac cggcggtttc caggcggatg cggtgtggcc gcgtccgtcc
     8461 cattctattt ccttctcggc ccacgcgaca aggacgtcac gctcctggcc gtccggcttg
     8521 gcgcggaatg cgtagacatg ggcgtccccg ccgggctgga atcggcccag ggcacgcgca
     8581 cccgccaggc atcggcccac ggcggccagg gccacatagg ccggacgcgg cgtcatgtcc
     8641 aggcgcagga ggccgaactg gaccccgtta ggctcgtgat aatggccaag aatgaagtgg
     8701 aaatggcgtc gggtgccggc gaacaggctg ctggcgtaag actgcgccag ccattcggcc
     8761 ttgagccgtt cgagtcgcga atcctggtcg taccaaggcc cctgtttgag atgcggggta
     8821 ccgcggtcgg cctcggtgac ccagagaggt cgtccgctca tggcctcgcg cgccggtttc
     8881 cagagttggg cgtaggagtg gggccagtcg taggtgtgga tgttgtaggt gtcgaagtaa
     8941 ggccaggtct cgttcgccag cacacccgcg gtctgggccg gagtcggggc ggccgtcgtg
     9001 acattccagc cgacgatcac cccgggatcg cccgctttga atccgagcca cgccgccttc
     9061 tgccaggaac acatctgatc gaccgtgtgc gcgccgaacg tggacacatt cgcctcgttc
     9121 caaggttccc aggccgcaac gcgccctttg aatcgggcgg ccaatgcgcg gccgaggtcg
     9181 aaagcatggc gcaaatcggg tgcgaaacgc ccgcctgcac tggactcctc gcgagcccag
     9241 gacggggtgt cgtgaaacac ctgcagcacc ttcagaccgg cggcatgctg cgcctcggcg
     9301 gcagtatcgt aagtcgtgcc cgccggcacg agggcgccgg gctcgggctg aatgtcactc
     9361 cagcgaagtc ggtcgcgaac ccaattgacg ccggcgagcg cggccaggtt cgcgaaccgc
     9421 tgttgccgag ggggatcgtt gcgcgcgaac cacgcagtcg ccgagtcgac acagacgggc
     9481 gaatcgcccg gcacgggagc gaccagccgt ctgatgaccg ccgcagtcgt ccacaggatg
     9541 tttgtccgtt ctggcgcatc gaactcgata cgataccagc cgatgtccag atcgcccgcg
     9601 gcgatgggcg tgcgcgcctg caccgcttct ccgctcagag cgcccgaccg cagcacacgg
     9661 gcctgatcgt cgagcagacg ccagcccgtg gccgtatcgg gcagcccttc gggcagccgc
     9721 acctgaaccg tttcgccttc gaggtagatg ttgccaggat gctcgggcaa cggcttgggg
     9781 atcgcccgtg tcgacggtgc gtccgccgat tcgtcggcct gcggatggag gcgggcgcgg
     9841 atgaccgccg ggttgtccgt gatgatgccg tcgacgccga ggcgcacgag ctgggcggcc
     9901 tcgttggggt cgttgatggt gtagacccag acgcgcagtc cgcgctggtg cgcgtgcgcg
     9961 atcgcatccg cggtgacttg gcgattccag acgaccaact gcgccccgag ttgttcgacg
    10021 tcggcaatga actcggggcc gagcaccttc tcctggtccg tgagcttgcg tccgttgcgg
    10081 ctcgaaggcg gccccaacgc gcccaggaca aggtcgggcg caacgcggcg ggcgtcccgg
    10141 acgaatcccc agtcgaaaga ctggaccacc agactatcga gccaaccgcg ctcgcgcagc
    10201 aggcgcacca aggctggcgc gtcccctcct ttacgttcga tcagcgtgac gccacccgcc
    10261 tgaatggtct cgatcgactc gaccaatgtg ggcagccgcg tcccggcgta cgcgggagag
    10321 aaccagcgcc cggcatcaag ggacgtgagt tcggacgcgt tccgcgaggt gacggcgatt
    10381 cccttcccgc cccagcggtt ggtggcgtcc gtggtccggt cgagtgtccc gtcgtggatc
    10441 acaacaggca cgccatcctt ggcatggtaa tagtcgagct cgaccaaatc ggcgcgcgcg
    10501 gccagcgcga ggcggaatgc cggcagcgtg ttttcggggg cctgctggct gaagccgcga
    10561 tgcgcgatca cgagcggtcc gcgctgggca atcagccgcg ccgcgggcga actggcgggc
    10621 ggggcgtctg aggcgtcggc aattgcgtga agaaagaacg gcgcgccaaa cagggccgtc
    10681 gcaaggaagg cgcgccccaa acgatcaggg acaaaagact cgatcatgat gttgtgctgt
    10741 cactctagtg atcgagagtg aacatgcaaa ccaaagcgct ctgtcattga ccacgggagg
    10801 gcatggcctg cgcccggagc cgtgcgcggg tccgtccatc ggggaacgag cccggacagg
    10861 cggtcagagc ggcgggcgag cgggatcgag ggtgacaggc cggtacacgc agaccaatgc
    10921 cggggggaga ttgaggagga caagttcgct gtcgacttgc tcgaaggcat cggagggcgg
    10981 ctggccgttg aggactcgat aggtaaactg aatccaacaa ccgccggggt caaggatcgc
    11041 gcggatgcac gcagcaagcc gggcgacgtt gggcgcggcg aagttattga gtggcaggct
    11101 ggacaccacg gcgccgaacc gctcgcctgg gaacaacgca tccagttcga gggcatcgcc
    11161 gggcacgaag cgcgcctcgg gaaaccgttc gcgcaggact tcgatcaggc agggcgactt
    11221 ttcgaccgcg atcaaccggc gcggatccag tccgcgcgcc aacaacgcct cggtcaccac
    11281 gcccgtgccc ggacccagtt cgagcacacg ccgcccgtta tccaccgagg cgcacttcag
    11341 accaacgcca gccctctgcc ccatggaggc tccgcgtgtt tctccgcccc tttctcccgc
    11401 gcctcgctcc ttggaaccct tgaccccgtc accgcagcac aaaagctcgc cctgtccgga
    11461 acgagtttca ccggtcaagg attccagagg gagccggcgt gccatggctc gagccagggc
    11521 gcgcgagctg ggccacacgg cgccgatttg gagaggatgc cgggcgagtt cggtgatgaa
    11581 ggcgcagagc ggatgggttc gatacggttc ccgcgcggag gcgcgactgg tgtccagact
    11641 tggcatgtca acaaacgaat tcgcggcact gtatcattgc gcgaatcgca gcgcacacta
    11701 tttctcttga gcgggcccaa cgcgggcggt caggagaagg tctgttccca cgcggtccca
    11761 ttcgatgtcc cgcagcgaga ccactgtggg ccaatcgcca aagcccgctc ccgccaccgc
    11821 cttccgcccg gccgcctcgc caagaacctt cggggcgtag tagaaagcga cgcgctgaac
    11881 cagcccggac tcgaaaagag acgcatggag ctcgccaccg ccctcgatca gcatggccgt
    11941 gacctcctca cgaccgagct ggcggagaat ccaatcgaga ttgagcttgg gacggcaggc
    12001 cgattggcgc gcgccccgat gccgcgagcc acggacacct ccccgttgct cgatctccgg
    12061 cgcgacgaca accctgaccc gccgagccag agcgtcccgg cgacgaaccg gagccgtcgg
    12121 gccgacgtac acggttgtcc aggccgccca gggatcggtg atcaggcgac aatccagagg
    12181 ggtccgcgcg cgggcatcca gaaccagtcg ccggatcggt ttgactggcg tcgagcaggt
    12241 ccgggccccg acgcctgttt ggagagtcgc gacggatcga atgccggcat cggcctcggg
    12301 tccatagccg ggccgagttg cgcctctggc cgtcaggctg ggatcatccg ccaacaccgt
    12361 gttcacgccc acgaggatgg catccgcgcc cagccgcaac cgcatggcgt ggcggcgggc
    12421 gattgcccct gtgatccact tcgattcgcc cgtcgccgcg gcaatcttgc catcgagtgt
    12481 catcgcggct ttgacggtca cgaatgggag gcgctcgaca atccagtggt tgaacgattc
    12541 gttgagccgc gctgcctcct ccgcaagcag cccgtgctcg accttcactc ccgcctgccg
    12601 cagcacctgg aaaccacgcc ccgcgtggtt cggattcgga tcggtggccg cggcgacgac
    12661 ccgggcaata tccgagcgtg tgatggcttc ggtgcagggc ggcgtgcgac ccgcggtgga
    12721 gcacggttcg agcgtcacat agagagtcgc cccgcgcacc gaacacccgc gccgggcggc
    12781 gtcgcccaac gcctcgatct ccgcgtgcgg ctgcccggcg cggtgatgcc agccctggcc
    12841 aatcacgcgt ccgttcttga ccaatacggc tccgaccatc gggttgggcg acgtctggcc
    12901 gtagcctcgc aaggcgaggc gcagggccag gcgcatgaag cggatgtcat tcggggcggc
    12961 agcgagtctc accgtcccgc gattaagcct gggacagact gtcaaggcaa gaggcatgct
    13021 tcgtttttcc gagcatcctg ttagtctccc tgcatgaaga agacacgcgg attccacatg
    13081 gttgtcgtca cggcgccgga tatgaagacc gcccgccgac tggcgcgggt tgcgctcgag
    13141 gcgcgtttga tcgcgtgcgc cagcctgatc ccccggatcg agtcacatta ctggtggaag
    13201 gacaggatcg agtcgggcag cgaggtcctg ttgctgatga agagcgccga ccggagcctg
    13261 tcggcactcg aacgacggat catggccgaa catccttacg acaccccgga gttcctggtg
    13321 tgcccgatcg cggggggcaa tgcgcggtac ttggcctggc tcgaggcgaa tgtcgtctcg
    13381 agccggtcgt ccgagcgccg ccggaggacc tgactttggc ttcaggcttc ggccatcgat
    13441 gtcaggactc cggcaggtaa cgttgggcga gcagcacggt gaggaagagg gcggcgaaga
    13501 ccagggttac gattgtggaa tggcctccga ccgccagaag atcgacgagc acgattccgg
    13561 cagtcaggcc ggagaccgtc atgcggatat tgggttctct ttcgcgcaag gcgtggctga
    13621 gccagggcag aatccagagg cacaacaaga ggcccagggc caccgccaca tggcgatccc
    13681 cgtcggcgtt gctgatccag gccaggccca ggggcacggc cagcaggacg agcggccaac
    13741 gccggattgg gccgcggatg ccttgacggc gggcgaacca actgaggccg gcgatgtagc
    13801 atccgatggc gaggccgctc cagatggcga tgcctgcgag gccgttgggg gcagtcgcgc
    13861 ctgccagcag gtagagcagg aaacggcaga agccgagcag caggggggac agctcggtcg
    13921 ccttgtgaat gagattgtag gcaaaaacgg cgaccgcaag catgagcgcg agcgtcagac
    13981 cgacgcgcgc gcccagcaca aggatggcgc ccccggcgat caaccagaga atgccgagct
    14041 ggcccacgac atcgcgggcg acgagtccgg ccgggatcgg ccggttgggg cgctgttggc
    14101 gatcgaagtc gacatcgaag aagtcgttga ggaaagtccc gcccaggtaa agacagctcg
    14161 cgcccaggct gcaccagagc aaacgcgccg gcgaatgggt gtcgccgccg ctcaggagcc
    14221 agcccgccag gcagttcgac cagacggtga tcaggccggg cagccgcgcc aggctgagca
    14281 acgtccgccc gtttggccag gtgtgaggca tcagtgtcgt tctcggggtt gatcgggcgg
    14341 attgcccgtc ccgagcggcg gatagcaaaa caggactcgg gagaaaagca atcccaagcc
    14401 cgggaatctc aggcagggac gaggacgcgg attcccgagc tgtggcaaaa ccgcgctcag
    14461 aaagggaata tggagcattc tgccggtcgt gaaggcgttt cgccctagga acagtgtgtt
    14521 gcggtcctct ttctctcgaa cctggggcgg ttt
//
LOCUS       DAOQRO010000031        14521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3224_length_14521_cov_5.428729, whole genome shotgun sequence.
ACCESSION   DAOQRO010000031 DAOQRO010000000
VERSION     DAOQRO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_14521_cov_5.428729"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="queG"
                     /locus_tag="PKK20_02260"
     CDS             complement(<1..730)
                     /gene="queG"
                     /locus_tag="PKK20_02260"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNU98743.1"
                     /translation="MKAALCQRARQLGFDACRVTSADPPPHAPRFIAWLEAGRHGTMA
                     YLLRTAAQRADPRILLSGARSVVTLAASYWQAPAPPSARASPQLRGLVARYARHADYH
                     DVLAQRIEQLAAFIATLDHPAPRSMACVDASPVLERDLAQRSGIGFIGKHTNLVSRQL
                     GNWFFLGEILTTLELEPDPPQANRCGSCARCLEACPTRALIAPFELDARLCIAYLTIE
                     LKGPIPAEVRPAIGNRIFGCDDCLS"
     gene            complement(727..1272)
                     /locus_tag="PKK20_02265"
     CDS             complement(727..1272)
                     /locus_tag="PKK20_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNU98744.1"
                     /translation="MNPDRTIRNLALIGFMGTGKSTVGHAAAYHLGFDFVDTDRLIEA
                     RAHKSVADIFAQDGEEVFRELERQVVEDLAQCDRTVIATGGGLGANLDHLVSLKRHAL
                     VVCLWTTADVIWERVRHHSHRPLLRVPNPRAHIRELLARREPFYRQADVLVNTGPRRL
                     RDVICHVLREFASARRSARRR"
     gene            complement(1269..2063)
                     /locus_tag="PKK20_02270"
     CDS             complement(1269..2063)
                     /locus_tag="PKK20_02270"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNU98745.1"
                     /translation="MILVLDIGNTHTHLGLATARRIDRSLDVATSGWFDGTAARAAAR
                     WVGSRPLEGVALCSVVPRATQRVRRALSAAWGIEPFELTYRTVRGLGIDYPHPASIGP
                     DRLANAVAARRRYGAPVLVLDFGTAVTLDVVNESGDYVGGIIAPGLAALTDYLHEKTA
                     LLPRVRIREVRKAIGRSTAEAMRIGAVLGYRGLIRTLVAEVRRELGCSRPPVVATGGY
                     ARLMARSLPEIMAVAPRLTLEGVLQVWQARRTPRTRQARGPLLPRR"
     gene            complement(2060..3040)
                     /locus_tag="PKK20_02275"
     CDS             complement(2060..3040)
                     /locus_tag="PKK20_02275"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNU98746.1"
                     /translation="MNMDAEILTALRSSGAGSLSGTELAQRLGVSRAAVWARIEDLRA
                     LGYEITASPHGGYRLLSTPDVLHADDLLSRLGKTRVIGRDIRVFRETTSTNDIIEKLA
                     RDGVREGVVVFAESQTKGRGRLGRRWVSPRGAGLWFSILLRPVLRPHEATQLTIMAAT
                     ALARAIREQTDFTLEVKWPNDILVGGRKVAGILTEPTAELDRIRHVVLGIGLDVNQAA
                     GDFPPDLRRIATSLRAEAGRPFDRAELAVTVLRALDRDYLRLKEGQFEGLAAEWEQLC
                     TTLGRDVTILLGSRRIRGRAESLDSEGALLVRTEHGRLERITGGDVTLAK"
     gene            complement(3037..3921)
                     /gene="nadC"
                     /locus_tag="PKK20_02280"
     CDS             complement(3037..3921)
                     /gene="nadC"
                     /locus_tag="PKK20_02280"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNU98747.1"
                     /translation="MIALSPQEIRTAVRRALTEDIGQGDATTLATVPAGARTQAHVVA
                     REQLVLAGLGIAAATFRCLSPRTRIQPLAADGDRIGTGQPVMRLEGPARALLTAERVA
                     LNFLQRLSGVATLTSRYVAAIAGTRARILDTRKTTPGWRRFEKYAVACGGGLNHRQGL
                     DDLVLIKDNHLAALAGEQPNPIAAAVMRARRRYPRLKVEVEADTLAQVKQAVDAGADI
                     ILLDNMSPRRMRRALTLIAGRALTEASGGIDLVSVRAVAATGVDFVSVGALTHSARAV
                     DLALDFAAAHQDSMTGSR"
     gene            complement(4375..4911)
                     /locus_tag="PKK20_02285"
     CDS             complement(4375..4911)
                     /locus_tag="PKK20_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNU98748.1"
                     /translation="MSNIVYFDLETQKSAEEVGGWNKIRDMRMSVGVTFSTARGTYQI
                     YPESRVNDLIEELRRADRVVGFNILRFDYEVLAGHSPFFDRSQIPTLDMLVELQKTLP
                     HRLSLDSIATATLGIEKTSDGMQAIRWFREGKLIEIAEYCCYDVKITKLVHEYGHTQH
                     HLIFKDRFGNTRKIPVSW"
     gene            complement(4976..6304)
                     /gene="lysA"
                     /locus_tag="PKK20_02290"
     CDS             complement(4976..6304)
                     /gene="lysA"
                     /locus_tag="PKK20_02290"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNU98749.1"
                     /translation="MHYFRYVDGHLHCEGLRVERLAREYGTPLYVYSQKTLEDHYGRL
                     DAVLAPLDNLICYAVKANSNLAVLRTFADLGSGFDIVSAGELERVIAAGGNPARCVFA
                     GVGKTEAEIALALRRGIYAFNVESEAELARIDRVAARLRKRAPVAVRVNPNVDAQTHA
                     KITTGTYENKFGIAFESVLPLYQRAARLKHLRLRGIQMHIGSQLTEVKPFESAVRRVA
                     PLAATLAKKYGIEFFSIGGGIGIVYESALASGQPDWWVTPAAKGLLTPVTYASKLVPL
                     LRPLGLKILVEPGRFLVGNAGILVTRIEYVKKTGEKRFLIVDAAMNDLVRPAFYDAYH
                     EILPLRCRCGAQALFDVVGPICESGDFFCRDRLLPAVDEGEYLALFSAGAYGAVMGSN
                     YNSRPFPPEILVSERRADCVRRRQPLERIWEGERAASWQARPASSRRRPR"
     gene            6397..7026
                     /locus_tag="PKK20_02295"
     CDS             6397..7026
                     /locus_tag="PKK20_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98750.1"
                     /translation="MDPHDFTVRRATLDDLPTLKGLWDVNRLPALDLERHLTEFQLVL
                     RADGVLAGAVGLRACGAQGLVHSEAFYSQQLADPVRSAAWQRLQILARNQRLGRLWIH
                     GEAAPGWQALGFKRAMAEDLKKLPPILGGELGPWFTVALIDETALAEVVERELTVFQQ
                     EARAENERLRQQALWFKWIAGLIVFGFLAGAVWVLYRVFSSQNRRRILR"
     gene            7054..10218
                     /locus_tag="PKK20_02300"
     CDS             7054..10218
                     /locus_tag="PKK20_02300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HNU98751.1"
                     /translation="MNPNSESSSNGGCACSGGWPRRDFLKAVGLGTAAAAVHPWEAMA
                     GPFTRADFEKLVPRDKKLRPEWVKSLFERGERTVYRGSDLDHIGMPIGGLCAGQVYLS
                     GDGRLMHWDIFNQHIGTGAEHYAKPMAMNQPFMQDFHIRVTSGTRSRVRRLCRDDWRD
                     VSFNGQYPIGRVEYRDPEFPVSVDLEAFSPFIPLNTADSSLPATVMRFRVRNTSDAGV
                     SVQLGGILQNAICLHTGKTRQGFRCNRLVREENALILEASAEDLPAGPSTARADIGFD
                     DFERESYGDWLAGGAAFGSGPVEMTRIPQYQGDVGGRGRRVVNSHASAPGGSVEEKDR
                     AQGTLTSRVFSIERDYITFLIGGGAHAGRTCMNLLIEGEPVLSATGANDNRMRPMSWD
                     VRRWMGKKASLEIVDAESGGWGNVGIDDIVFSDRPREPLGPLANETDFGTICLALLEA
                     RPDDVGQTNLTELTGLFNSDKTSAAPTRKPFGDDPIGGLTRTITLAPNTEAAVTFLLT
                     WHMPNLKMDRLPPGRHYATRFESAAAVARYIARHLARLSGETRLWHDTWYDSTLPYWF
                     LDRTFLNTSILATSTCHRLGNGRFWAWEGVGCCHGTCGHVWQYAQAMARLFPELERIT
                     REQVDFGLAQQKNGAIYFRGEFNNIPAIDGQAGTILRALREHQTSADAAFLKRNWPRI
                     RRAMEWLIAKDEDGDGLITGNQHNTLDTDWYGPVAWLSGLYLAALAASEAMAREVGDQ
                     EFGDRCHAILDVGRAKLVHELFDGDYFINKPDPKHAEAINSGSGCHIDQVMGQSWAFQ
                     VGLPRVLPETETRAALRALWRYNFTPDVGPYRTAYKPGRWYAMPGEAGLLMCTFPRTD
                     WDYEQAKGKGADWAAGYFNECMNGFEYQVAGHMIWEGMVQEGLAITRAVHDRYHASRR
                     NPWNEVECGDHYARSMASYGVFLAACGFSYHGPKEHLGFAPRLTPEQFKAAFTTAEGW
                     GSFEQKRTPGGLEAAIDLKWGTLRLRVVKLKPADGTVPASLRVRAAGRDVPAILEMRE
                     GQAEIRFNDLVELKRGERLVLTLG"
     gene            complement(10204..11205)
                     /locus_tag="PKK20_02305"
     CDS             complement(10204..11205)
                     /locus_tag="PKK20_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNU98752.1"
                     /translation="MLSLSMPFRRLGGLVCTAVAILLFLHTTNAADGSGVRITDLDGK
                     LRIEIGGELFSEYRYRGFSRPFLYPVLGPAGLPMTRNWPMKEVEGEERDHPHQRSFWW
                     AHGAVNGHDFWSEGANAGRTLHDKFLEVKSGPEFGVIRAANKLVAKDGTLIATDERTI
                     RIHNRTADRIVDFEITIHASEGPLTFGDTKEGTMALRLNEAMRLTRNRKPAEGHIVNS
                     EGLRDGATWGKRAKWVDYYGPVEGQTVGVAIFDHPWNPRHPTHWHVRDYGLFAANPFG
                     IHDFEKKPAGTGDLEVAAGQSVTWRYRFYFHRGDEQEAKVAERYAEYAQVSPASAQR"
     gene            complement(11237..11644)
                     /locus_tag="PKK20_02310"
     CDS             complement(11237..11644)
                     /locus_tag="PKK20_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98753.1"
                     /translation="MRIHRTDPYPEVLGEFLGSAAFGQQTEYVLLAHRERLADATLAG
                     RLVKRRHHPARNGAGHRRAPALELGHGFEDLLRPGMFDQVARGAGLQGFEHPVDVFLD
                     CEHDDLRARQESPQPGDTLDAGRAGLWCVELSC"
     gene            complement(11751..14078)
                     /locus_tag="PKK20_02315"
     CDS             complement(11751..14078)
                     /locus_tag="PKK20_02315"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98754.1"
                     /translation="MTRSHLFLACALWASASGIPVHPGPVEPELKSTVEADWAAQERR
                     LGREPGSIEALEAAVVRGQSLLNILASALPPAELDIERRAWESLRTEARAAGRLPSAE
                     RIALYHRVRWSGRVLALRNPAVTGQPIAFMKRHRFVCQMLHEYLGYFYDYGDVAGGGV
                     FVLERPGVSFEVRDLIAGRLPRGNYTTLALGYDARTLYFAFAERALRKPDFNSADRRC
                     FQLYAIEVDGSGLRRLTDGPNDDFDPCPLPDGALAFMSTRRGGYGRCHNEWEPLPAYT
                     LHRMNKDGSDVKTLSWHETNEWHPFVLHDGRIVYTRWDYVDRSAANFHGLWASNPDGS
                     NPVSLFGNYTTRINACYQPRAIPGSQRIVFVAGAHHADVGGSLVIVDPQRAALDPETG
                     EDRLEDMEVLTPEVCFAEAAGWPKSYFHSPWPLSEDWFLVAFSHEPLPGMSSGELRDT
                     RTGLYYLDRAGNLELLYRDPEASSMYPIPLAPRPAPPAIASTLDPALGDEGEFLLTDV
                     RLSLRPMPTDRPVRQLRVFQVLPKTHTHVANQPRIGHANAESARLLLGIVPVESDGSA
                     YFRAPARKPLYFQAVDAAGRAIQSMRSVTYLQPGERRGCVGCHEPRRNTPITRPTLAS
                     KRPPSHLEPGPDGSCPLSYPRLVQPVLDRHCVRCHDGSTGADTSALILTGDPAPPFTR
                     SYQNLRPFLRWFEWGGASIAEASTPPGRIGADASPLTGILEDPTHRTEAALPDGDRRC
                     LYLWLDANAPFYGTYSKDEQLAQFEGRAVPPPKVQ"
     gene            complement(14133..>14521)
                     /locus_tag="PKK20_02320"
     CDS             complement(14133..>14521)
                     /locus_tag="PKK20_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNU98755.1"
                     /translation="NAPEHRLVPEKFRWEPEVFHKAMKKLTAALRPWTIDFHVAQNDA
                     TVKGSGAHEKTGKHCMATDPNGKLNIARDAGYWMRDGAGKVTRKFKHICWDGCMFPNA
                     IMTQQQTWNDILAAMIQVRENHGWKA"
BASE COUNT         2601 a         4478 c         4961 g         2481 t
ORIGIN      
        1 ccgacaggca atcgtcgcaa ccgaagatgc ggttcccgat ggcgggacgc acctcggcgg
       61 gaatggggcc tttgagttcg atggtcaaat acgcgatgca gagccgggca tcgagttcga
      121 acggggcgat caaggcgcgc gtggggcagg cctcgagaca ccgggcgcag gaaccgcagc
      181 gattggcctg aggcgggtcc ggctcgagtt caagcgtggt gagaatttcg cccagaaaga
      241 accaattgcc gagctggcgg ctgacgaggt tggtgtgctt gccgatgaag ccgattccag
      301 accgctgtgc caaatcccgt tcgagcacgg ggctggcatc gacgcacgcc atcgagcggg
      361 gggcggggtg atcgagcgtg gcgatgaagg cagctagctg ctcgatgcgt tgggcgagga
      421 cgtcatggta atcggcgtgg cgcgcgtagc gggcgacgag gccgcggagt tgcggtgacg
      481 cccgggcaga cggcggggcc ggggcctgcc agtaactcgc cgcgagcgtg acgacgctgc
      541 gggctccgga cagcagaatc cggggatcgg cgcgttgggc ggcagtccgg agcaaatagg
      601 ccatcgtccc gtgccgaccg gcctcgagcc aggctatgaa tcggggggca tgggggggcg
      661 gatcggcgct ggtaacgcgg catgcgtcga atccgagttg gcgggcacgc tggcagaggg
      721 ctgctttcat ctgcgccggg ccgagcgcct ggctgaggcg aattcgcgga ggacgtggca
      781 gatcacgtca cggagtcgac gcgggccggt gttcacgagg acgtcggcct gtcggtagaa
      841 cggctcgcgt cgggcgagga gttcgcgaat atgcgcccgg gggttgggaa ctcggaggag
      901 cgggcgatgg gaatggtggc ggacgcgttc ccagatcacg tccgcggttg tccagaggca
      961 gacgaccagg gcgtggcgtt tgaggctgac gaggtgatcg agattggctc cgaggccgcc
     1021 ccccgtggcg atcacggtcc ggtcgcactg agcaagatcc tcgaccacct ggcgctcgag
     1081 ttcgcgaaaa acctcctcgc catcctgcgc gaagatgtcc gcgacggact tgtgcgcgcg
     1141 cgcttcgatg agccgatccg tgtcgacgaa gtcgaagccg aggtgatacg cggcggcgtg
     1201 gccgacggtg gacttgccgg tgcccatgaa accgatgagc gccagattcc ggatcgtgcg
     1261 atcaggattc atcggcgcgg caagagggga ccacgcgcct gtcgggtgcg aggcgtgcgg
     1321 cgggcctgcc agacttgcag cacgccttcg agcgtcaggc ggggcgcgac cgccatgatc
     1381 tccggcaggc tgcgcgccat gagccgggcg tagccgccgg tggccacgac tggcgggcgg
     1441 gagcagccca attcgcgccg cacttcggcg accagcgtgc ggatcagacc gcggtaaccg
     1501 agcacggcgc cgatccgcat ggcctcggcg gtgctgcgtc cgatggcctt gcgcacctcg
     1561 cggattcgaa cgcgcggcag caacgcggtc ttttcgtgga ggtaatcggt gagggcggcc
     1621 aaccccgggg cgatgatgcc gccgacgtag tcgccggact cattgacaac gtcgagggtg
     1681 accgccgtgc cgaaatcgag aaccagcacc ggggcgccat atcggcggcg cgcagccacc
     1741 gcgttggcga ggcggtccgg cccgatcgat gcgggatggg gatagtcgat ccccagaccg
     1801 cgcacggtgc gatacgtgag ttcgaagggt tcaatgcccc aggcggccga caacgcgcgg
     1861 cgcacgcgtt gggtggcgcg aggcacgacg ctgcagagag ccaccccttc cagagggcgg
     1921 ctcccgaccc atcgggcggc ggccctggcc gcagtgccgt caaaccaccc ggatgtcgcc
     1981 acgtcgagac ttcggtcgat tcgccgcgcc gtggcgagcc ccaggtgcgt gtgggtgttc
     2041 ccaatgtcga gaacaaggat catttggcga gcgtgacgtc gccgccggta attcgttcga
     2101 ggcggccgtg ctcggtccgc accaacagcg cgccttcgga atcgagcgat tcggctcgcc
     2161 cgcgaatgcg ccgggagccg agcagaatgg tgacatcgcg gcccagggtg gtgcagagct
     2221 gctcccactc ggcggcgagc ccctcgaatt gcccctcctt gagccggaga taatcgcgat
     2281 caagcgcgcg caggacggtg acggccaatt cggcccggtc aaaggggcgg ccggcttcgg
     2341 cgcgcaacga cgtggcgatg cgccgaaggt ccggcggaaa gtcgccagcc gcctggttca
     2401 cgtccagccc gatcccgagc acgacatgcc ggatgcgatc cagctcggca gtgggttcgg
     2461 tcaggatgcc ggccactttg cgtccgccca cgaggatgtc gttgggccat ttcacttcca
     2521 gcgtgaaatc ggtctgctcc cgaatcgcgc gcgccagcgc ggtggccgcc atgatcgtga
     2581 gctgagtggc ttcgtgcgga cgcagcacgg ggcggagcag gatcgagaac cagagtccgg
     2641 ccccgcgggg cgagacccag cggcgtccga ggcgaccgcg ccctttggtt tgagactcgg
     2701 cgaacaccac gacgccttcc ctgacgccgt cgcgggcgag cttctcgatg atgtcgttgg
     2761 tcgatgtggt ctcgcggaag acacggatgt cgcgaccgat cactcgagtc ttgccgaggc
     2821 gcgacagcag atcatccgcg tgcagaacgt cgggcgtgct caggaggcgg tagccgccgt
     2881 gcgggctggc cgtgatctcg tagcccaacg cccgcaaatc ctcgatgcgc gcccacacgg
     2941 ccgcgcggct gactcccaga cgctgcgcca actcggtccc ggagagggaa ccggcgccgg
     3001 agctgcgcaa cgcggtcaga atctcggcgt ccatgttcat cgcgagcctg tcatcgaatc
     3061 ctgatgcgcg gcggcaaaat ccagcgccag atcgaccgcc cgcgccgagt gggtcagcgc
     3121 gccaacggag acgaagtcga ccccggttgc ggccacggcg cgcaccgaca ccaggtcgat
     3181 gccgccgctg gcctcggtca gggccctgcc ggcgatcaga gtcagggcgc gacgcatccg
     3241 ccgcggggac atgttatcga ggaggatgat atcggcgccg gcatccacgg cctgtttgac
     3301 ctgggcgagg gtgtcggctt cgacctcgac cttgagccgc ggataccgcc gccgggcccg
     3361 catgacagcg gcggcgatgg gattgggttg ttcgccggcc agagcggcaa ggtggttgtc
     3421 cttgatcagc acgagatcgt cgagtccctg gcggtggttg agtccgccac cgcacgcgac
     3481 ggcgtacttc tcgaagcggc gccatccggg tgtggtcttg cgggtatcga ggatgcgcgc
     3541 gcgcgtgccg gcgatggcgg ccacgtagcg ggaggtgagc gtcgcaacgc ccgagagccg
     3601 ctgcaggaag ttcagggcga cccgctcagc agtcagcagc gcccgggcgg ggccttccag
     3661 gcgcataacc ggctggcctg tcccgatgcg atccccgtcg gctgcgagcg gttggatgcg
     3721 agttctgggc gagaggcacc ggaaggtcgc cgcagcgatg cccaagccgg cgagcaccaa
     3781 ctgctcacgg gcaaccacat gcgcctgggt gcgggcgccg gccgggacgg ttgccagggt
     3841 tgtggcatcg ccttgcccaa tgtcttcggt caaggcccgc cgcaccgccg tccggatttc
     3901 ctggggtgaa agtgcgatca ccaatgcatc atcccccagg acaggcccag gatgaaggca
     3961 aaaaagcggg atacccgcgg caccagtgga tcagcgagtc tccggctccg ccgcggtcac
     4021 aactccatga accatgcagg cgcatttgca tttctcccgt tttgagacac tgttgcccgc
     4081 actggctcgg gtttcggagt cttccgcttc gcggcggtca ggacacgatg aagcccgcgg
     4141 gccagccctg atggccagaa cgagctatgg acgtgctata cttgatcggc gtgcttgccg
     4201 cgtggtttgt attgatgaag tgggtgttgc cgcgattcgg cctccgcacc tgaatgtcgg
     4261 acggttgctc ggtcgagcaa catcgcaaac accccgaatc cgcccgccgc gaatccgcac
     4321 ccccggaatc ggacaaggac gtttgagttc actggcgccg gtacggcgtc gggatcacca
     4381 gcttaccgga atcttcctgg tgttgccgaa gcggtccttg aagatcaggt ggtgctgggt
     4441 gtgaccgtat tcgtggacga gcttggtgat tttcacgtcg tagcagcagt attcggcgat
     4501 ctcgatcagc ttgccttccc gaaaccaccg gatggcctgc atgccgtcgc tcgtcttttc
     4561 gatgcccaag gtagccgtgg caatcgaatc gagtgagagc cgatgcggca gcgttttctg
     4621 cagctcgacc agcatatcga gcgttgggat ttgggatcga tcgaagaacg gagaatggcc
     4681 ggccaggacc tcgtagtcga accgcaggat attgaatccg acaacacggt cggcccggcg
     4741 caactcctcg atcaggtcgt tgacgcggga ttcggggtag atctggtagg tcccgcgggc
     4801 ggtgctgaag gtgacaccga cgctcatgcg catgtcgcga atcttgttcc agccgccgac
     4861 ctcctcggcg gacttctgcg tttcaagatc gaagtagacg atgttgctca caggctgggt
     4921 ctggcttagc agggagtgcg tccccgctca ataccggagt cgatcgaagg acagttcacc
     4981 gcggccggcg gcggctcgag gccggccggg cctgccagga cgcagcccgt tcgccttccc
     5041 agatgcgttc gagaggctga cgtcgccgca cgcaatcagc ccgacgctcg ctgaccagaa
     5101 tctccggggg aaacgggcgg ctgttgtaat tcgagcccat gaccgcgccg taggccccag
     5161 cgctgaacaa cgcgaggtat tcgccttcgt ccaccgccgg caaaagccgg tcgcggcaaa
     5221 agaaatcgcc cgactcgcag atcggtccga ccacgtcgaa caaggcttgc gctccgcagc
     5281 ggcagcggag cgggaggatt tcgtgatacg cgtcgtagaa tgccggacgc accagatcgt
     5341 tcatggcggc gtcgacaatg agaaaccgtt tctcgcctgt cttcttcacg tactcgatgc
     5401 gagtgacaag gatgcccgcg ttgccgacga gaaaccggcc gggctcgacc aggattttca
     5461 ggcccagcgg acgcagcagg gggacgagct tcgatgcgta cgtgacgggg gtgagcagtc
     5521 ctttggccgc gggcgtcacc caccagtcgg gctggccgct ggccagagcg gattcgtaga
     5581 cgatgccaat cccgccgccg atgctgaaga actcgattcc gtatttcttc gccagcgtgg
     5641 cggccaaagg cgcgacccgg cgcaccgccg actcgaacgg tttcacctcg gtcagttggg
     5701 atccgatgtg catttggatg ccccgaagcc gcagatgctt gagccgggcg gcgcgctggt
     5761 agagcggcag aacgctctcg aaagcgatcc caaacttgtt ctcgtaggtg ccggtggtga
     5821 tcttggcatg ggtctgagcg tcgacattcg ggttgacgcg cacggccaca ggcgcccgtt
     5881 tccggagccg agccgcaacc cggtcgatcc gcgccaactc ggcctcgctc tcgacgttga
     5941 acgcatagat cccgcggcgc agggccaggg cgatttcggc ttccgtcttc ccaacaccgg
     6001 caaagacgca tcgcgccggg ttgccgcccg ccgctatgac acgctcgagt tcgcccgcgc
     6061 tgacgatgtc gaaacccgat ccgaggtcgg cgaaggtcct caagacggcg aggttcgagt
     6121 ttgccttgac ggcgtagcaa atgaggttgt ccagcggagc caacaccgca tcgaggcgtc
     6181 cgtaatggtc ttcgagagtc ttctgggaat agacatagag gggtgtgccg tactctcggg
     6241 cgagtcgttc cacccgcaat ccctcacagt ggagatgccc atccacgtac ctgaaataat
     6301 gcatgatccg catctctgcc agactcgagg gctcttgtca cgactggaac accataaagc
     6361 tttacccctg accgcaccac gcgtaggttc ggacccgtgg atccacacga tttcacggtg
     6421 cgccgcgcca cgctcgatga cctgccgacg ttgaaggggc tctgggatgt caatcggctt
     6481 cccgccctcg atctcgaacg ccatctgacg gagtttcaac tcgtcttgcg ggcggacggc
     6541 gtgcttgcgg gagcggtggg gctgcgcgcc tgcggcgccc aggggctggt ccacagcgaa
     6601 gcgttctatt cacagcaact cgccgatccg gtccggtccg cggcctggca gcgcctgcaa
     6661 atcctggccc gcaaccagcg cttgggccgg ctctggattc atggcgaagc cgcccccggc
     6721 tggcaggcgc tcggattcaa gcgggccatg gccgaagacc tgaagaagct gccgccgatt
     6781 ctgggaggcg aactggggcc atggttcacc gtcgcgctga tcgacgagac tgcgttggcc
     6841 gaggtggtcg agcgagagtt gactgtcttc cagcaggagg ccagggccga gaatgagcgc
     6901 ctgcggcagc aggccctctg gttcaagtgg attgccggcc tgatcgtgtt cggcttcctg
     6961 gccggcgccg tatgggtcct ctaccgcgtg ttctcctccc agaatcgccg ccgaatcctc
     7021 cgctgaagca gaggcaattc gagaaggcat cctatgaatc cgaactcgga atcgtcttcg
     7081 aacgggggct gcgcgtgttc aggcggctgg ccgcgtcgcg actttctcaa agccgtcggc
     7141 ctgggaacgg ctgcggctgc agtgcatcca tgggaggcca tggccggccc gttcaccagg
     7201 gccgatttcg agaaactggt cccgcgcgac aagaagctgc gtccggaatg ggtgaaatcg
     7261 ctgttcgagc gtggcgagcg gaccgtctat cgggggtcgg acctggatca catcgggatg
     7321 ccgattggcg ggctatgcgc gggccaggtg tatctgagcg gcgacgggcg gcttatgcac
     7381 tgggacatct tcaaccagca cattggcacg ggggcggagc attacgccaa gccgatggcg
     7441 atgaaccagc ctttcatgca ggactttcat atccgcgtga cctcgggcac aaggagccgg
     7501 gtccgccgac tgtgccgcga cgactggcgc gacgtatcgt tcaacgggca gtaccccatc
     7561 gggcgcgtcg agtatcgtga tccggagttc ccggtcagtg tcgatctcga ggcgttttcg
     7621 ccattcatcc ctttgaatac ggcggattcg tcgctcccgg caacggtcat gcgcttcaga
     7681 gtgcgcaaca cgagcgatgc gggggtttca gtgcagctcg gtgggatact gcagaatgcc
     7741 atctgccttc acacagggaa gacccggcag ggattccgct gcaaccggct ggtccgtgag
     7801 gaaaacgcat tgatcctcga agcctcggcc gaggatttgc ccgcggggcc gtccacggcg
     7861 cgagccgaca tcggtttcga cgatttcgag cgggagagct atggcgattg gctggccggc
     7921 ggcgcggcgt tcgggagcgg gccggtcgaa atgacgcgca ttccgcagta ccagggcgat
     7981 gtggggggac gagggcgtcg ggtggtcaac agccacgcct cggcgccggg cgggtcggtc
     8041 gaggagaagg accgggcaca ggggacgctt acgagcaggg tcttctcgat cgagcgggac
     8101 tacatcacgt ttctgatcgg cgggggggcc catgcaggcc gcacgtgcat gaacttgctg
     8161 atcgagggtg aaccggtgct gagcgcgacc ggcgccaatg acaaccgaat gcgcccgatg
     8221 agctgggacg tgcgccgctg gatggggaag aaggcgtcgc tcgagattgt ggatgcagaa
     8281 tctggcggct ggggcaacgt ggggatcgac gatattgtgt tcagcgatcg gccgcgagaa
     8341 ccgctcggcc cgctggcgaa cgagacggat ttcgggacca tctgtttggc gttgctcgaa
     8401 gcgcggcctg acgacgtggg acaaaccaat ctgacggagc tgacgggctt gttcaactcc
     8461 gacaagacgt ccgcggcccc gacgcgcaag ccctttggcg atgatccaat tggcggtttg
     8521 acgcgcacca tcacgctggc gcccaacacg gaggccgctg tgaccttcct gctgacctgg
     8581 cacatgccga acctcaagat ggatcggctg ccgccgggcc ggcattacgc gacgcggttc
     8641 gagtctgccg cggccgtggc ccggtacatc gcacggcatt tggcgaggct cagtggcgag
     8701 acgcggttgt ggcacgacac gtggtacgac tcgaccctgc cctattggtt cctcgatcgg
     8761 acgtttctga acacttcgat tctcgccacc tcgacgtgcc atcggctcgg aaacggtcgg
     8821 ttctgggcgt gggagggcgt cggttgctgc cacggcacgt gcggtcacgt ctggcaatat
     8881 gcccaggcga tggcgcggct gtttcccgaa ctcgaacgaa tcacgcgcga acaggtggat
     8941 ttcggcctcg cgcagcagaa gaacggcgcg atctatttcc gcggcgagtt caacaacatc
     9001 ccggccattg acggccaggc cggcacgatc ctgcgcgcgc tgcgcgaaca ccagacgtcg
     9061 gccgacgcgg cgtttctgaa gcgcaactgg ccgcggatca ggcgggcgat ggaatggttg
     9121 atcgcgaagg acgaagacgg cgacggactg atcacgggca atcaacacaa caccctggac
     9181 acggactggt acggcccggt ggcatggctc agcggactgt accttgcggc gctcgccgcc
     9241 tccgaggcga tggcgcgcga ggtgggcgat caggagttcg gcgacaggtg ccatgcgatc
     9301 ctcgacgtcg ggcgcgcgaa gcttgtgcac gaactctttg acggcgacta cttcatcaac
     9361 aaacccgatc ccaaacacgc cgaggcgatc aactccggga gcgggtgtca catcgatcag
     9421 gtcatgggac aaagctgggc gtttcaggtc ggacttcctc gcgttcttcc cgagaccgaa
     9481 acgcgtgcgg cattgagggc gttgtggcgc tacaacttca cgccggacgt ggggccgtat
     9541 cggacggcct acaagccggg gcgctggtac gcgatgcccg gcgaggcggg gttgttgatg
     9601 tgcacattcc cgcggacgga ctgggattac gagcaggcga agggaaaagg ggcggactgg
     9661 gcggcaggct acttcaacga gtgcatgaat gggttcgaat accaggtcgc gggtcacatg
     9721 atctgggaag ggatggtcca ggagggactg gcgatcacgc gggcggtgca tgaccgctac
     9781 cacgcctccc ggcgcaatcc gtggaacgaa gtcgagtgcg gcgaccatta tgcccggagc
     9841 atggcgagct acggcgtgtt tcttgccgcc tgcggcttct cctatcacgg ccccaaggaa
     9901 cacctgggct ttgcgccgcg gttgacaccg gagcaattca aagcggcgtt cacgaccgcg
     9961 gaaggctggg gatcattcga gcagaagcgg acaccgggcg gactcgaagc ggcgattgac
    10021 ctcaagtggg ggacgctgcg attgcgcgtc gtgaagctga agccggcgga cggaacggtg
    10081 ccggcatctc tgagggtgcg agccgccggg cgcgacgtgc ctgcaatcct tgagatgcgg
    10141 gaaggccagg ccgagattcg tttcaacgat ctggtcgaac tgaaaagggg cgagaggctc
    10201 gttctaacgc tgggctgatg ccggactcac ctgcgcgtac tcggcatacc gctcggccac
    10261 tttggcttcc tgctcgtcgc cgcgatggaa gtagaagcgg tagcgccacg tgacactctg
    10321 gcctgccgcc acttcgaggt caccggtccc cgccggtttc ttctcgaagt cgtggattcc
    10381 gaacggattc gccgcgaaca aaccgtagtc gcgcacgtgc cagtgcgtgg gatggcgcgg
    10441 attccacgga tgatcgaaaa tggcgacacc gacggtttgg ccttccacag gtccatagta
    10501 atcaacccac ttggcgcgtt tgccccaggt agctccgtcg cgaagcccct cgctattcac
    10561 gatgtgcccc tcggccggct tcctgttgcg cgtcaggcgc atcgcctcgt tcaggcgcag
    10621 agccatggtc ccttccttcg tgtcgccgaa cgtcagcgga ccttccgaag catgaattgt
    10681 gatctcgaaa tccacaatcc gatcggcggt gcggttgtga atgcggatgg tgcgttcgtc
    10741 cgttgcaatg agcgtgccgt ctttggccac cagcttgttg gccgcccgga tcacgccgaa
    10801 ctccggaccg gacttcacct cgaggaactt gtcatggaga gtcctgccgg cattggctcc
    10861 ctcggaccag aaatcatggc cgttgacggc accgtgggcc caccagaacg aacgctggtg
    10921 agggtgatcg cgctcctccc cctcgacttc cttcatcggc cagttgcgcg tcatgggcag
    10981 gccggccggg ccgaggaccg ggtaaaggaa cggacgggag aagcctcggt agcggtactc
    11041 ggagaagagt tctcctccga tttcgatgcg gagcttgcca tcgagatcgg tgatccgtac
    11101 tcccgagcca tccgcggcgt ttgttgtgtg caggaagagg agaatggcga ccgcggtgca
    11161 gacgaggcct ccaagacgtc gaaagggcat gcttaggctg agcatcgggg cgagcttagg
    11221 aagcggaagc ggatggtcaa caagaaagct cgacacacca aagcccggcg cgtccggcat
    11281 cgagtgtgtc gcccggttgt ggcgactcct gccgcgcacg caggtcatca tgctcgcaat
    11341 cgaggaagac atcgaccggg tgttcgaatc cctgaaggcc ggcgccacgg gctacctggt
    11401 caaacatgcc aggccggaga agatcctcga agccgtggcc gagttccagc gcgggagcgc
    11461 gccgatgtcc agcgccattg cgcgccgggt ggtgacggcg tttcaccagc cgccctgcga
    11521 gggtggcgtc ggcgaggcgc tcacggtgcg cgaggagaac atactccgtc tgttggccaa
    11581 aggctgccga tccaaggaat tcgccaagca cctccggata ggggtcagta cggtgaatac
    11641 gcacgtccgc catctattgg aaactgcacg tccgctcccg tgcggaggcc gtggcgcgct
    11701 acatggggcg gtgagcgccg ccccctgggt tggcgtcgag cgccgctcgt tcactggacc
    11761 ttcgggggcg gcacagcccg gccctcgaat tgggcaagct gctcgtcttt cgaataggtg
    11821 ccgtagaaag gcgcgttggc atccagccag agatagaggc agcgccgatc cccgtctggc
    11881 agcgccgcct ctgtgcgatg cgtcgggtcc tccagaatgc cagtcagcgg gctggcgtcc
    11941 gccccgatgc gacctggagg cgtcgaggcc tctgcgatcg aggcgccgcc ccactcgaac
    12001 caccgcagaa acggcctcag gttctggtag gatcgcgtga agggcggcgc cggatcgccc
    12061 gtgagaatca gcgcgcttgt gtcggcgccg gtcgatccgt cgtggcagcg gacgcaatgc
    12121 cggtcgagga cgggttgcac cagccgtgga tagctcaacg ggcaactgcc gtcaggaccg
    12181 ggttcgaggt gtgaaggcgg gcgtttcgag gccagggtcg gacgcgtgat cggcgtattg
    12241 cgccgcggct cgtggcaacc cacacagccc ctgcgttctc ccggctggag gtaggtcaca
    12301 ctgcgcatgc tctgaatggc gcgaccggcg gcgtcgacgg cctgaaagta cagcggcttg
    12361 cgggccggag ctcggaaata agccgatccg tccgactcca ccggcacgat ccccaggagc
    12421 aggcgggcgc tctcggcatt tgcatggccg atgcggggtt gattggcgac gtgcgtgtgc
    12481 gtcttgggga ggacttgaaa gacccggagc tgacgaaccg gacggtcggt aggcatcggg
    12541 cggaggctca ggcggacatc ggtgaggagg aattcgccct cgtcgcccaa agccggatcg
    12601 agcgtgctcg caatggctgg gggcgccggc cgcggagcca gggggatcgg gtacatcgag
    12661 gacgcctccg gatcgcggta gagcagttcg aggttccccg cgcgatccag gtaatagagc
    12721 ccggtgcgcg tgtcgcgcaa ttcgcccgaa ctcatgccgg gcagcggctc gtggctgaaa
    12781 gcgaccagaa accagtcttc cgacaacggc caggggctgt ggaaatagct cttcggccat
    12841 cccgccgcct cggcgaaaca gacctccggc gtcaggactt ccatatcctc gagccgatct
    12901 tcgcccgtct ccggatcgag cgcggcgcgc tgcggatcga caatcaccag cgagccgcca
    12961 acgtcggcat gatgggctcc ggccacgaag acaatccgct gggagcccgg gatggcgcgc
    13021 ggctgatagc atgcgttgat ccgcgtcgtg tagttgccaa acagcgacac cgggttcgag
    13081 ccgtcgggat tgctggccca gagaccgtgg aaattcgccg ccgagcgatc gacgtaatcc
    13141 cagcgcgtgt acacgatccg accgtcgtga aggacaaacg gatgccattc gttggtctca
    13201 tgccacgaga gcgttttcac atccgatccg tccttgttca tccggtgaag cgtgtaggcc
    13261 ggaagcggtt cccactcatt gtggcaccgg ccatagccgc cgcgccgggt cgacatgaac
    13321 gcgagggcgc catcgggcag cggacaagga tcgaagtcgt cgttgggacc gtcagtcaac
    13381 cgacgcaagc cggagccatc gacctcgatc gcgtacaact ggaaacagcg ccgatcggcc
    13441 gagttgaagt ccggcttgcg cagcgcgcgc tcggcaaacg cgaagtagag cgtgcgcgcg
    13501 tcatagccga gcgccagcgt ggtgtaattg cctcggggca gccgacccgc aatcagatcg
    13561 cgcacctcga acgagacccc gggccgttcg agcacaaaga cgccgccgcc ggccacgtcg
    13621 ccgtagtcgt agaagtaccc gaggtattcg tgcagcatct ggcagacaaa ccgatgccgc
    13681 ttcatgaaag cgatcggctg ccctgtaacg gcaggattcc gcagtgcaag gacgcgtccg
    13741 ctccaccgca cgcgatggta caaggcaatc cgctcagcag acggcaaccg accggccgct
    13801 cgcgcctcgg tccgcagtga ttcccatgcg cgcctctcga tgtccaactc cgcgggtggg
    13861 agtgccgacg ccagaatgtt cagcagactt tgcccccgca ccaccgcggc ttcgagggcc
    13921 tcgatgctcc cgggctcgcg gcccaaacgg cgttcctgag ctgcccaatc cgcctcgacc
    13981 gtcgacttga gctcaggctc gaccgggcct gggtgcaccg ggatgccaga agccgaggcc
    14041 cagagcgcac aggccaaaaa gaggtgggac ctggtcatca agcaagataa gcccaggcat
    14101 tagatcacgc ccagccccgt aaaatgtccg tgctaagcct tccagccgtg gttctcgcgc
    14161 acctggatca tggcggcgag gatgtcattc catgtctgtt gctgtgtcat gatggcgttg
    14221 gggaacatgc atccatccca gcatatgtgt ttgaacttcc tggtcacctt gccggcgccg
    14281 tcccgcatcc agtaaccggc atcgcgggcg atgttgagct tgccgttcgg atcggtggcc
    14341 atgcagtgct tgccggtctt ctcatgggcg cccgatccct tgactgtcgc gtcgttctgc
    14401 gccacatgga aatcaattgt ccacggacgc aaggccgccg tgagcttctt catggccttg
    14461 tggaagacct ccggttccca tctgaacttc tcggggacca accggtgctc cggagcattg
    14521 g
//
LOCUS       DAOQRO010000032        14446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3248_length_14446_cov_4.497325, whole genome shotgun sequence.
ACCESSION   DAOQRO010000032 DAOQRO010000000
VERSION     DAOQRO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_14446_cov_4.497325"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1393
                     /gene="rho"
                     /locus_tag="PKK20_02325"
     CDS             272..1393
                     /gene="rho"
                     /locus_tag="PKK20_02325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNU98756.1"
                     /translation="MSNNSHVPETGSGYLEISEKGFGFLRTSEGHFHPKPTDVFVTPD
                     TIKKCYLREGCFVKSTLQAPHRGSSPQLKEVLEVNGLPFAEYTKAMRFENLTTIDPFE
                     KFRLETAPDLLETRIIDLVTPIGRGTRGLIVAPPRTGKTTILKQIANAVTTNHPEVHV
                     MVLLIDERPEEVTDFQRSVKAEVVASSNDQDLETHVRLSRFMIERCRRMVESKRDTFI
                     LLDSITRVARAYNSVHGGSGRTMTGGVDARALEIPRKIFAAARKIEEGGSLTIVATAL
                     VDTGSRMDELIFQEFKGTGNMELVLDRKLSDRRLFPAIDIPKSGTRKEEKLFPPNQID
                     AIRKLRRMMVDLQPVEAMETLLAALKKHRTNDELLVKLQ"
     gene            complement(1464..2570)
                     /locus_tag="PKK20_02330"
     CDS             complement(1464..2570)
                     /locus_tag="PKK20_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNU98757.1"
                     /translation="MNSYNIAVIGGDGTGPEVVREAIKVLDAAARKFSIRLDYTHFDF
                     GGDRYLRTREVLPDSAVAELRQFPAILLGAIGHPDVKPGILEKGILLRLRFELEQYIN
                     LRPVKLYDQRFCPLRDKGPQDIDFVVVRENNEGLYTGVGGFVFKGTPNEVAVQESVNT
                     RRGIERCLRFAFDLARKRRGGAPWPGLKSDDVAAGRKAQLTLCGKTNVLTYAFDLWER
                     AFHQIGQKNYPDIKRDYAHVDATTMWFVKNPEWFDVIVTDNLFGDIITDLGAMIQGGM
                     GIAAGGNINPTGTSMFEPIGGSAPKYTGLGVINPLAAICAGQMMLDFLGETEAAGAIE
                     KAVIRTVVEKIKSLAAGKMGYSTSQVGDLVAAAL"
     gene            2744..4831
                     /locus_tag="PKK20_02335"
     CDS             2744..4831
                     /locus_tag="PKK20_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNU98758.1"
                     /translation="MKEYASADIRSFVIGGHATCGKTMLGEAMLACSGMIGRLGSIAN
                     GTTVSDYHESEQKRKISIHATLLHTDWLGKKFNIIDTPGYMDFISEAMAALRIADFAL
                     VVVHPIDGIALGTNAVWQYATEFGLPKMLVVNMLDKEHTNFDGVLSQLRERYGSKVFP
                     ITLPVNPGPGFNQVLDVMRSVITTYTTDGTGKLTEAAAAGEWADKAKALHQELIEHIA
                     EADDTLLEKFFEQGGLSEDELHAGLHTAVQKGEVIPLFATSGETNVGVARLMDFIAKY
                     GASPLDRPQLEAVDANDQSVQVKLTDPAPVIYVFKTLNEEHIGEVSIFRVQSGTVRFG
                     SEMFNANRNTSERIGQILVLNGKNRQNVNSLSAGDLGAVVKLKNTHTGDTLCAPGTNL
                     RLPKAAFPKPNIHAALKLKSKGEEDKIAQGLAALHEEDPTFVYAVDSELHQTVLSGQG
                     ELHLQVISERLRSRYKVDFDLVAPRIRFRETIKTKADSKYRHKKQTGGAGQFAEVWMR
                     IEPKPRGTGVEFTQSLVGQNVDRAFVPSVEKGVNAACTEGILAGYRVVDLKIDFYDGK
                     MHPVDSKDIAFQIAGKEAFREAFMAGRPCLLEPIHVVEIRVPDDAMGNVIGDLSSRRG
                     KILGMDTAGGFQVIKAAVPAMELYQYSTVLRSLTGGRAAHTEDLDHYEEMPKEMEQKV
                     IAEAKKAKEEQQA"
     gene            complement(4922..6874)
                     /locus_tag="PKK20_02340"
     CDS             complement(4922..6874)
                     /locus_tag="PKK20_02340"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNU98759.1"
                     /translation="MNASLRPARLFAQLAVIVALSRCGAAQSTSPAAPEPQIAGDKAL
                     TASPGPVSIDGRLSEPQWDGAEAIRSFSFPWSSRPAPATEFRAWADADHLYFSFTAED
                     DDLVVETRFDGESTLDREDRVEIFFARDEAPARMFNGVVTAAKPKTPECGPRRARKQN
                     RACPGESEEGKTVSGWRSSPSDRPLPSAAHPGAVLPTSPDQVQRGASDRSGLARYYCI
                     EIDPLGRVHDYAAQHYRQFDSSWNCPGLRAAGTRGSRGYTVEAAVPFATLSDLMGRAT
                     GPGATLRIGLFRAEFRPGALGDAPDNWISWVRPATTEPDFHVPSAFREWRIPASSDEA
                     TPAFRTRGIVLVPDDLSLADWPDRAAQAGLTTIALHHGTSPAAVAAFIGSPPGRAFLD
                     RCAQLGLEVEYELHAMNELLPRSLFAAEPLLFRMNEQGERTADANLCVHSARALEIVS
                     TNALRLARQLRPTTGRYFFWGDDGLPWCRCPRCREFTESDQALILENHLVRVLRQIDP
                     AAQLAHLAYHNTLPAPRRVKAEPGVFLEFAPIHRSYDKPYHEQTGPDVRDALAALEAN
                     LRVFPTHSAQVLEYWLDVSRFSGWKRPAVKLPWRRDVIEADAATYARLGIRHVTTFAV
                     WIDADYVRRFGEPTALAEYGAALRNR"
     gene            complement(6917..9046)
                     /locus_tag="PKK20_02345"
     CDS             complement(6917..9046)
                     /locus_tag="PKK20_02345"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNU98760.1"
                     /translation="MTHWIYAGIAVLLVVSTLGTRAVTVSGEELHEARRWVAARFLGR
                     APAPAPDPGTGLAVDASSTENARGGQRLSFFTTDPPFSFVYDGRPSAEAIASWQRALR
                     SRDLDDQRTEHSLCLTDPKTGLELRCVAVEYRDFPTIEWTLYFRNTGTVDTPILEQIQ
                     PLDALMDRGEGGEFLLHHAVGSPADGNDYGPRETLLAPGTTRRIGAAGGRPTNTDWSY
                     FNLEWPPGRGLIVVVGWPGQWVAEFARDTGLGLRLRAGQELTRFKLLPGEEIRTPLIV
                     LQFWQGDWIGAQNVWRRWMMAHSMPKPGGTLPPPQLVASSSRQFDEMIRANETNQIQF
                     LDRYLEEGLKLDYWWMDAGWYIHHGGGWPRVGTWLVDTNRFPRGLRAVSDHAHRKGVK
                     ILVWFEPERVTAGTWLTENHPEWVLGGAQGGLLNLGDTSARQWLIEHVDRLLTEQGID
                     LYRQDFNMDPLPFWRKNDAPDRQGITEIKHVTGYLDYWDELRRRHPNLLIDSCASGGR
                     RNDLETMRRAVPLWRSDYAYEAIGHQCMTYGISLWLPFHGTGTVATRNAPYYGSGVTP
                     VEPYAFWSNVTPSLGCGFDMREPLDYAALRRLVGQWRRIAPNFSGDFYPLTPWSRDDT
                     TWMAWQFHRPESGRGLVQVFRRHNSHYESARFPLRGLKAETLYRVENVDTGAATDMAG
                     RELAEAGLPVSIAEKPGAAVLTYTEIQ"
     gene            complement(9119..10534)
                     /locus_tag="PKK20_02350"
     CDS             complement(9119..10534)
                     /locus_tag="PKK20_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNU98761.1"
                     /translation="MLFLFRSVRLHVAVCILGATIPAARSGQAAVLPGLVNVRDHGAV
                     ADGRTLDTAALNAAIVACAGAGGGQVHFPPGRYLSGTLHLRSGVTLHLDSGARLIGTT
                     NLAEYSQPNVPDVLPEARWGKWHRALILAENAEDLGIGGPGIIDGNRVFDPTGEERMR
                     GPHTIVLVNCRRFTLRDVTIIDSANYALFAQVSDCMDIRQVTFIGGWDGVHWRGAPQR
                     WCLDVRITGCRFYTGDDAIAGRYWDNTVISDCIINSSCNGIRLIGPATGLIVDRCLFY
                     GPGEEPHRTSREQRRTNMLSGIILQPGAWDATQGPLDDVLLSNNTMRNVASPVTLWIK
                     PGNTVGRVTVAGLTATGVYRSALSVESWADTPVTNVVLRNLSVEWAGGGTAAQAKQPV
                     RGPGVDARPLPAWGIYARHVETLTVEDARLSLATPDLRPVIFAEKVGRLVLDHARFPR
                     LDGVEQPVATDEVKSLTVVPP"
     gene            10712..12676
                     /locus_tag="PKK20_02355"
     CDS             10712..12676
                     /locus_tag="PKK20_02355"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNU98762.1"
                     /translation="MKQLLFSWFTTAACVGGFAAETVWLHTLDLTPMRQGYGTPQIDR
                     GIREQPLSIAGQKFEHGVGTHAKSTLWINLAGGSSRFQAAVGVDDATGDPKASVTFKI
                     VGDGRTLWQSGTMKMGEPAKSVDLDVTGIQTLILAVGDAGDGIAFDHANWAEARFQVA
                     GATPKTMAQPSEPAVILTPVPGPSPRINGPRVYGCRPGNPFLYRIPTTGERPIRFAAA
                     GLPDGLRLDASSGIISGTSLKRGSYTVTLRAANSHGHDKRTLKIVCGDTLALTPPMGW
                     NHWYTHYDRITDELMRQAADVMVSSGMADVGYQYVNIDDCWMNASKNRDSLRVGPLRD
                     DRGNIVPNKHFPDMKALSDYIHGKGLKAGLYTSPGPLTCAGFAGSHEHEAQDARQFAE
                     WGFDFLKYDWCSYDGVVKGDHSLETLKRPYRLMGDLLKQQNRDIVFNLCQYGMGNVWE
                     WGEEVGGHCWRTAGDLGFELDRVFEVALKNAEYRAHSKPGSWNDPDYIQIGLIGNARG
                     MGEPIPCPLTPNEQYSFMSLWCLMAAPLFFSGDMNHLDAFTVNVLCNPEVIEIDQDPL
                     GECARVIPAGEETFLMVKNLEDGDKAVGLFNQGETETTVTARWSDLGVKGRQPVRDLW
                     RQKNLGRFRNAFAARVPARGCVLVRLDCGR"
     gene            12735..14057
                     /locus_tag="PKK20_02360"
     CDS             12735..14057
                     /locus_tag="PKK20_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNU98763.1"
                     /translation="MDIKLPKLGEGADSGVVVTVFVKEGDTVAKDQPLLELENEKAVA
                     SIPSPADGTVAQVFVKPGDKLSIGQRILTLGGEGAPGASKGVASAAVASARGVRSEPA
                     PAKAAPRPGAAVEPEPEGAQEPGAPEDSADDKPAAPPAASPSIRRLAKELGIDLTRIR
                     GSQRGGRIVMADLRAYIERLVRLAARPAATAAQPSKPAAEPIDFSKWGPVTTKPLTPL
                     RQTISRRMAESWAAVARVTQFDEVDVTRLLALRKQYLAAYEERGTKLTLTGFILKALA
                     AVLTRHPIFNASLDATGENLVFKSYYHIGIAVDTDAGLLVPVIRDADKKTLVELSRDL
                     ETVAMKARERKVTLDDLKGGTFTLSNQGGIGGAHFTPIINLPEVAILGIGRGAVKPVW
                     IKDHVEPRTLVPLAVSYDHRVIDGGNAARFMVDLVQTIEGMGEDAVKL"
     gene            14088..>14446
                     /locus_tag="PKK20_02365"
     CDS             14088..>14446
                     /locus_tag="PKK20_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU98764.1"
                     /translation="MERIQTELVVVGAGPGGYTAAFYAADRGKQVMLVERERRLGGVC
                     LNCGCIPSKALLHAAHTISLTRESAHRGMTFAPPQLDLGKLRAWKESILQRLGNGVSQ
                     LAQKRGVRVLEGRGYFE"
BASE COUNT         2744 a         4542 c         4682 g         2478 t
ORIGIN      
        1 gatcggggtt catatgattc aaggcactgt ctgtccggag taaaggccag gcgggggcaa
       61 agcgtcaagc ttactcgtcc gcgcgcaaaa taaatgttgc gggcgggcgg ctttggacct
      121 agatattgct cgtcttaggt ccaactgttc ggccctgcaa cccggttcca tgaacctctt
      181 cggccggcgg ttttcccgaa aattgaaatt gaaggcgcag ccaagcggag cccctccggc
      241 gagagatcga cggggcgcga acacaagcat tatgagcaac aacagccacg tgccggaaac
      301 cggttccgga tatctcgaaa tctcggaaaa aggtttcggt ttcctgcgga cgtccgaggg
      361 gcattttcat cccaagccaa cggacgtatt cgtcacgccg gacacgatca agaagtgcta
      421 cttgagggag ggctgctttg tgaagagcac gctgcaggcg ccgcaccgcg gcagcagccc
      481 ccagctcaag gaggtcctcg aagtcaacgg actgcccttc gctgagtaca ccaaagcgat
      541 gcggttcgag aacctgacga ccatcgaccc cttcgagaag ttcaggctcg agaccgcccc
      601 ggacctgctc gagacccgca tcatcgatct ggtcacaccc attgggcgtg gaacgcgcgg
      661 gctcatcgtc gccccgccgc gcacgggcaa gaccacgatt ctcaagcaga ttgccaacgc
      721 cgtgacaacc aaccacccgg aggtgcatgt gatggtgctg ttgatcgacg agcgccccga
      781 ggaagtcacc gacttccagc gttcggtgaa ggccgaggtc gtcgcctcct cgaatgacca
      841 ggacctcgaa acccacgtcc gcctctcccg cttcatgatc gaacgctgtc gccgcatggt
      901 cgaatccaag cgtgacacgt tcatcctcct cgactcgatc acccgcgtcg cccgcgccta
      961 caacagcgtc catggcggaa gcggtcgcac catgaccggc ggcgtcgacg cccgcgcgct
     1021 tgaaatcccc cgcaagattt tcgccgccgc gcgcaagatc gaggagggcg gatccctgac
     1081 cattgtggcc accgcgcttg tcgacaccgg atcgcgcatg gacgagctga tctttcagga
     1141 gttcaaaggg actggaaaca tggagctggt gctcgatcgg aagctttcgg atcgccggct
     1201 cttcccggcc atcgacatcc ccaagagcgg cacccgcaag gaagagaagc tctttccacc
     1261 caaccagatc gacgccatcc gcaagctccg ccgcatgatg gtcgacctcc agccggtcga
     1321 ggccatggaa accctcctcg ccgcgctcaa gaaacaccgc accaacgacg aactgctggt
     1381 caagctgcag tagggaggcg accccgtccg cgggaacacc tcgcgcttgt gtcttgttgt
     1441 ctccgcgccg cgcaaggcgc ggctcagagc gccgctgcga ccaggtcgcc cacctgcgag
     1501 gtgctgtagc ccatcttgcc cgccgccagg ctcttgatct tctcgacgac ggttcgaatc
     1561 acggccttct cgattgcacc agccgcttcg gtctcgccca ggaaatcgag catcatctgg
     1621 ccggcacaga tggctgccag cggattgatc acccccaaac ctgtatactt gggcgcgctg
     1681 ccgccaatag gctcgaacat cgaggtgcct gtcgggttga tgttcccgcc cgccgcgatg
     1741 cccatgccgc cctggatcat cgccccgaga tcggtgatga tgtcgccgaa cagattgtcc
     1801 gtcacaatca cgtcgaacca ctccggattc ttcacaaacc acatcgtcgt cgcatcgacg
     1861 tgcgcgtagt cccgtttgat gtcgggatag ttcttctgcc cgatctggtg gaaggcgcgt
     1921 tcccaaagat cgaacgcgta cgtgagcaca ttggtcttgc cgcagagggt cagttgggcc
     1981 tttctgcccg ccgcaacatc gtcggatttg agcccaggcc agggcgcgcc cccgcgccgt
     2041 ttgcgcgcaa gatcgaatgc gaagcgaagg cagcgctcga tgccgcgccg cgtattgacg
     2101 ctttcctgca ccgccacttc gttcggcgtg cccttgaaca cgaacccgcc caccccggtg
     2161 tagaggcctt cgttgttctc gcgcaccacc acgaaatcga tgtcctgcgg tcctttgtcg
     2221 cgcagcgggc agaagcgctg atcgtagagc ttcacgggac ggaggttgat gtattgttcg
     2281 agttcgaaac gcaaacgcag gagaatgcct ttctcgagaa tgccgggctt gacgtccgga
     2341 tggccgatgg cccccaagag aatcgcggga aactggcgca actcggcgac ggcgctgtcg
     2401 gggaggactt cccgagtgcg gaggtaacgg tctccgccga agtcaaaatg ggtgtagtcg
     2461 aggcggatcg agaacttgcg ggcggcggca tcgaggacct tgatggcttc ccggacgact
     2521 tccgggcccg tcccgtctcc gcctatcacg gcgatattgt agctgttcat gggtgttcga
     2581 ctgcgtggcg tccccccagg ggcgcaacag aggcacgcat cgtaggaact cggcgcccaa
     2641 gttcaatccg aaaaattgcg aatttgctgg cgaacgacag gatttcccgt acaaaagcgg
     2701 cttttagaga agacggaccc agattcgaag cgctcaagtg tctatgaaag agtacgcatc
     2761 agcggacatt cgcagttttg tcatcggcgg ccacgcaacg tgcggcaaga ccatgctcgg
     2821 cgaggccatg ctggcctgca gcgggatgat cggacgcctc ggcagcatcg ccaacggcac
     2881 caccgtgtcc gattatcacg agagcgaaca gaagcggaag atttccatcc acgccaccct
     2941 gctccacacc gactggctcg ggaagaagtt caacatcatc gacactccag gctacatgga
     3001 tttcatcagc gaggccatgg ccgcgctgcg aatcgccgac ttcgctcttg tggtcgtcca
     3061 cccgatcgac ggcatcgccc tggggaccaa tgcggtctgg cagtacgcga ccgaattcgg
     3121 tctccccaaa atgctggtgg tcaacatgct cgacaaggag cacaccaatt tcgatggcgt
     3181 cctttcgcaa ctgcgcgagc gctacggatc caaggtgttc ccaatcaccc tgcccgtgaa
     3241 tccaggcccc ggcttcaacc aggtgcttga cgtgatgcgc agcgtgatca ccacctacac
     3301 taccgacggc accggcaaac tgaccgaggc cgccgccgcc ggcgagtggg ccgacaaggc
     3361 caaagccctc caccaggagt tgatcgaaca catcgccgag gccgatgaca cactcctcga
     3421 gaagtttttc gagcaaggcg gcctctcgga agacgagttg cacgccggcc tccacaccgc
     3481 cgtccaaaaa ggcgaggtca tccccctctt cgccacctcc ggggaaacca acgtcggcgt
     3541 cgcgcgcctc atggatttca tcgccaagta cggggcgtcc cctctggatc gccctcaact
     3601 cgaagcagtc gacgccaacg atcagtcggt tcaggtcaag ctcaccgatc ccgcgcccgt
     3661 gatctatgtc ttcaagacgc tcaacgaaga acacatcggc gaggtctcca tcttccgcgt
     3721 tcaatccggc acggtgcggt tcgggagcga gatgtttaat gccaaccgca acacgagcga
     3781 gcggatcggc cagattctcg tgctcaatgg caagaatcgg cagaatgtca acagcctcag
     3841 cgccggcgac ctcggcgccg tggtcaagct caagaacacc cataccggcg acacgctctg
     3901 cgcgccgggc accaacctcc ggctccccaa ggcggccttc cccaagccca acatccacgc
     3961 cgccctcaaa ctcaaatcca agggtgagga agacaagatc gcccaaggcc tggccgcgct
     4021 gcacgaggag gatccgacct tcgtgtacgc ggtcgattcc gagctgcacc agacggtcct
     4081 ttccggccag ggcgaactgc atctgcaggt gatctccgag cggcttcgct cccgttacaa
     4141 ggtcgatttc gacctcgttg ccccccgcat tcgattccgc gaaaccatca agaccaaggc
     4201 ggattccaag tatcgacaca agaaacagac gggcggcgcc gggcagttcg ccgaggtctg
     4261 gatgcgcatc gagcccaaac cgcgcggcac gggcgtcgag ttcacccaga gcctcgtcgg
     4321 ccagaacgtc gaccgcgcct tcgtcccgtc ggtcgagaag ggagtcaatg ccgcctgcac
     4381 cgagggcatt ctcgccggct atcgcgtggt cgatctcaag atcgatttct acgacggcaa
     4441 gatgcatccg gtggactcga aggacatcgc cttccagatc gccggcaaag aagcgttccg
     4501 cgaagccttc atggccggac gcccctgcct gctcgaaccc atccatgtcg tcgaaatccg
     4561 cgtccccgac gacgccatgg gcaacgtgat cggcgatctc tcgagccggc gcggcaagat
     4621 tctgggcatg gacaccgccg gcggattcca ggtcatcaag gccgccgttc ccgccatgga
     4681 actctaccag tattcgaccg tcctgcgctc cctcaccggc ggacgcgctg cccacaccga
     4741 ggatttagat cactacgagg agatgcccaa ggagatggag cagaaagtca tcgccgaggc
     4801 caagaaggcc aaggaagaac agcaggcgta gccctcagat tcaccggtta gcgcggacat
     4861 ttcaaatcgc tgcgcgtgtc ggatatgaaa ctccgcaaag cccgatgaag catatctcgg
     4921 gctagcggtt gcgcagggct gcgccgtact cggccagggc cgtcggctcg ccaaaacgcc
     4981 ggacataatc ggcgtcgatc cagaccgcga acgtcgtgac gtgccggatt cccaggcgcg
     5041 cataggtggc ggcgtccgcc tcgatcacgt cgcggcgcca gggcaacttg accgcggggc
     5101 gtttccatcc ggaaaaccgg gagacgtcca gccagtactc gaggacctgc gcgctgtgcg
     5161 tcggaaacac ccgcagattc gcctcgagtg ccgccagcgc gtcgcggacg tccggcccgg
     5221 tctgctcgtg atacggcttg tcatagctgc ggtggattgg ggcgaattcg aggaagacac
     5281 ccggctcggc tttgactcgg cggggcgcgg ggagcgtgtt gtggtaggcc aggtgcgcca
     5341 gttgggccgc gggatcgatt tggcgcagca cccgcaccag gtggttctcg aggatgagcg
     5401 cctggtccga ttccgtgaac tcgcggcagc gcggacaccg gcaccacggc aaaccgtcgt
     5461 cgccccagaa gaagtaccgg ccggtggtgg ggcgcaactg gcgcgccagc cggagagcgt
     5521 tggtggacac gatttcgagc gcgcgcgctg aatgcacgca gaggttggcg tcggcggtgc
     5581 gctcgccctg ctcgttcatg cggaaaagga gcggctccgc ggcgaagagg ctgcgtggca
     5641 gcagttcgtt catcgcgtgc agttcgtatt cgacctcgag gccaagctgc gcgcagcgat
     5701 cgagaaaggc gcgtccgggc ggcgacccaa tgaacgcggc caccgcagcc ggggaagttc
     5761 catggtgcaa cgcgatggtg gtcaggccgg cctgtgccgc ccgatcgggc cagtcagcca
     5821 gcgataggtc gtcgggcacg agcacgatgc cgcgggtgcg aaaggcgggt gtggcttcgt
     5881 cgctggaggc cgggatgcgc cactcccgaa acgccgatgg cacatggaaa tccggttcgg
     5941 ttgtcgcggg ccgcacccaa ctgatccagt tgtccggcgc atcgcccaag gcgccggggc
     6001 gaaactcggc gcggaacaga ccgatgcgca gtgtcgcgcc cggtcccgtt gcacgaccca
     6061 tcaagtcgct gagcgtggca aagggaaccg cggcttcgac tgtgtagccg cgcgagccgc
     6121 gcgtgcccgc cgcgcgcaga ccaggacaat tccaggagct gtcgaactga cggtagtgtt
     6181 gggcggcata atcgtgcaca cgccccaggg gatcgatctc gatgcaatag tagcgggcta
     6241 gcccggatcg atcagaggct ccgcgctgaa cctgatccgg gctagtaggc aaaaccgcgc
     6301 ctggatgggc ggcagaaggt aaaggtcgat cgctcgggga acttctccat cccgacacag
     6361 tcttgccttc ctcagattct cctgggcagg cgcgattttg ctttctggcc cgccgcggtc
     6421 cgcattctgg tgttttcggc ttcgcggcag tcacaactcc gttaaacatc cgggctggcg
     6481 cctcgtcgcg ggcgaagaaa atctcgaccc gatcctcgcg atcgagcgtc gattcgccgt
     6541 cgaaccgcgt ctcgaccacc agatcgtcgt cctccgccgt gaaactgaaa tagaggtggt
     6601 ctgcgtccgc ccaagcccga aactcggtcg ccggtgcggg ccgggatgac caggggaagg
     6661 agaaggaccg gatcgcttcg gcgccgtccc actgcggctc actcagccgg ccgtcgatcg
     6721 agaccggccc gggactggcc gtcagcgcct tatcacccgc gatttggggc tcgggggcgg
     6781 caggggatgt cgattgggcg gctccgcaac gactcaaggc gacgatgacg gccaactgcg
     6841 caaacagcct ggcgggacgg agagacgcgt tcatgtccac aggattctga gagggacttg
     6901 ttccccggtc aattggctac tgaatctcgg tgtacgtgag aacggcggcg ccgggtttct
     6961 cggcgatcga gaccggcagg ccggcttcgg cgagttcgcg tccggccatg tccgtggcgg
     7021 cgcctgtatc gacgttctcg acacgataga gtgtctcggc tttcaggccg cggaggggga
     7081 agcgagccga ctcgtaatgg ctgttgtggc gccggaagac ctgcacgagg ccgcgtcccg
     7141 attcgggccg atggaattgc caggccatcc aggtcgtgtc atccctggac cacggggtga
     7201 gggggtagaa atcgccggag aaattgggcg cgatccgtcg ccattggccg acaaggcggc
     7261 gcagggcggc gtaatcgagt ggctcgcgca tgtcgaagcc gcagcccagg ctcggggtga
     7321 cgttgctcca gaaggcgtag ggttcgacgg gggtgacgcc gctgccgtag taaggcgcat
     7381 tgcgtgtggc cacggtgcct gtgccgtgaa agggcagcca gagcgagatg ccgtaggtca
     7441 tgcactggtg gccgatggcc tcgtacgcgt agtcgctgcg ccacaacggc accgcgcgac
     7501 gcatcgtctc gagatcgttg cgccgtcctc ccgaggcgca tgagtcaatc aggaggttcg
     7561 gatgccgacg gcgcagttcg tcccagtagt ccaggtagcc ggtgacatgt ttgatctcgg
     7621 tgatgccctg gcggtcgggg gcgtcgttct ttcgccagaa gggcagggga tccatgttga
     7681 aatcctggcg gtagaggtcg attccctgct ccgtcaggag tcgatccaca tgctcgatca
     7741 accattggcg cgccgaggtg tcgccgagat tcaacagacc gccctgggca ccgcccaaga
     7801 cccactcggg atggttctcc gtcagccagg tgccggccgt gacgcgctcc ggctcgaacc
     7861 agacgaggat tttgacgccc tttcggtgcg catgatccga gacggcccgg agcccccgcg
     7921 gaaagcggtt cgtgtccacc agccaggtgc ccacacgcgg ccagccgcct ccgtgatgga
     7981 tataccagcc ggcgtccatc caccaatagt cgagcttcaa tccctcttcg agatagcggt
     8041 cgaggaactg gatttggttt gtctcgttgg cccggatcat ctcgtcgaac tggcgcgaac
     8101 tcgatgcgac cagttgcggc ggcggcaggg tgccgcccgg cttgggcatc gagtgggcca
     8161 tcatccagcg ccgccagacg ttttgcgcac cgatccagtc tccctgccag aattgcagca
     8221 cgatcaaagg tgtccgaatt tcctcgcccg gcagcagttt gaagcgggtc agttcctggc
     8281 cggcgcgcaa acgcaggccg agtccggtgt cccgggcgaa ctcggctacc cactggccgg
     8341 gccagccgac gaccacgatc aggccgcgcc cgggcggcca ttccagattg aagtacgacc
     8401 agtcggtgtt ggtgggccgc ccgccggcag cgccaatgcg ccgggttgtg cccggggcga
     8461 gcagcgtctc gcgcggcccg taatcgttgc catccgcagg ggagccgacg gcatgatgca
     8521 ggaggaattc gccgccctcg ccacggtcca tcagcgcgtc caagggctga atctgctcga
     8581 ggatgggcgt gtcgacggtg cccgtgttcc tgaaatacag agtccactcg atcgtgggga
     8641 agtcgcgata ctcgacggcg acgcagcgca gttcgaggcc ggtcttcgga tcggttaagc
     8701 agagcgagtg ctcggtgcgt tggtcatcga ggtcccggga gcgcagggcg cgctgccagg
     8761 acgcgatggc ctcggccgag gggcgcccat cgtaaacaaa cgaaaacggc gggtcggtcg
     8821 tgaagaaaga cagccgctgg cctccccgtg cgttctcggt cgaggacgcg tcgaccgcca
     8881 agcccgttcc cgggtccggg gctggtgcag gggcgcgccc taggaatcga gcggcaaccc
     8941 atctgcgcgc ttcgtgcaac tcctcgccgg agacagtgac cgctcgagtg ccgagcgtgg
     9001 agacgacaag tagaacagcg atccccgcat aaatccagtg tgtcatggat gaccttctac
     9061 tcgaacccga gccgggtgaa cagctccggg attccggacg cgttcgcggt caggacggtc
     9121 acggaggaac gacggtgagg gacttgacct cgtcggtggc gaccggttgc tcgacgccgt
     9181 caagacgcgg gaagcgcgca tggtcgagca caagacgccc caccttctcc gcgaaaatca
     9241 ccggacgcaa gtcgggcgtc gcgagactca gacgcgcatc ctcgactgtc agtgtctcga
     9301 catgccgcgc atagattccc caggcgggaa gcggacgggc atcgaccccg ggccccctca
     9361 ccggttgctt cgcctgtgcc gccgtccccc ctccggccca ttcgacgctc aggttgcgga
     9421 gcacgacatt ggtgacgggt gtgtcggccc agctttcgac ggagagcgcg gagcgataga
     9481 cgcccgtggc ggtcaggccg gcgacagtga cgcgcccgac ggtattgccg ggtttgatcc
     9541 agagcgtcac gggagaggcc acgttgcgca tcgtattgtt cgacagcaga acatcatcga
     9601 gcggaccctg ggtggcgtcc caagcgccgg gctgcaggat gatgcctgaa agcatgttcg
     9661 tgcgccgctg ttcgcggctg gtgcgatgag gctcctcccc cgggccgtaa aacagacagc
     9721 gatcgacgat caggcccgtc gcgggaccga tcaggcgaat cccgttgcag gacgagttga
     9781 tgatgcaatc ggaaatgacg gtgttgtccc agtaccgtcc ggcaatggca tcgtcgcccg
     9841 tgtaaaaccg gcagccggtg attcggacgt cgaggcacca gcgctgcggc gcgccgcgcc
     9901 aatggacgcc gtcccagccg ccgatgaatg tgacctggcg aatatccatg caatcgctca
     9961 cctgggcgaa cagggcatag ttggccgaat caatgatggt cacgtcgcgc agggtgaacc
    10021 gccggcaatt gaccagcaca atcgtatgcg gtccgcgcat ccgttcctcg cctgtcgggt
    10081 cgaacacacg gttgccgtcg atgattccgg gtccgccgat gccgaggtct tccgcgttct
    10141 cggccaggat cagtgcccga tgccatttgc cccacctggc ctcgggcagg acgtcgggta
    10201 cgttcggttg gctgtactcg gccaggttcg ttgtgccgat caatcgggcg ccggaatcca
    10261 gatgcagcgt gaccccgctg cgcaagtgga gcgtgccgga caggtagcgg cccgggggga
    10321 aatgcacctg gccgccgccg gccccggcgc acgcaacgat cgccgcgttg agcgccgcag
    10381 tgtccagggt gcgcccgtcc gccacggcgc catggtcgcg cacgttgacg agcccgggca
    10441 agacggcggc ctgccccgat cgagcagcag gaatcgtcgc gcccaaaatg cagacggcca
    10501 catgcaggcg cacagagcgg aaaaggaata gcatgagccg agtattggca tcgacagccc
    10561 gctgtgtcga gcaacggcga gacggcttca tgcagggttc ttgcttttcg ggcttgtgga
    10621 ccaaggcgcc cccagcctac gtttggcgct catccggatt ccaacagggt cacggttctt
    10681 ggcgctcaga acgagaacga caggcacttc gatgaaacaa ctcctttttt cctggttcac
    10741 aacggcggct tgcgtcggcg gtttcgccgc ggagaccgtc tggttgcaca cccttgatct
    10801 caccccgatg cgtcagggct atggcactcc gcaaatcgac cgcggcatcc gcgagcagcc
    10861 gctctcgatt gccggccaga agttcgagca cggcgtcggg acccacgcga agagcacgct
    10921 ctggatcaac ctggcgggcg ggtccagccg gtttcaggcg gcggttgggg tcgacgacgc
    10981 cacgggcgac cccaaggcct cggtgacatt caagatcgtg ggcgacggcc gcaccctgtg
    11041 gcaatcgggc accatgaaga tgggcgagcc ggcgaagtcc gtcgacctcg atgtcacggg
    11101 gatccagacc ttgatcctgg cggtgggaga cgcgggagac ggaatcgcat ttgaccatgc
    11161 gaactgggct gaagcgcggt ttcaagttgc cggcgcgacg cccaagacca tggcccaacc
    11221 gtccgagccg gcggtgattc tgacgcctgt gcccgggccg tccccgcgga tcaacgggcc
    11281 gcgggtctac gggtgtcgtc cagggaaccc gtttttgtac cggattccga ccaccggaga
    11341 gcgcccaatc cggtttgccg ccgccggtct gcccgacggg ctgcgattgg acgcgtcgag
    11401 cgggatcatc agcggcacat cgctcaaacg cggcagttac acggtgacac tgcgggcggc
    11461 gaactcgcac gggcacgaca agcgcactct gaagatcgtg tgcggcgaca cgctggcgtt
    11521 gacgccgccg atgggatgga accactggta cacgcattac gaccggatca cagacgaact
    11581 gatgcggcag gccgctgatg tgatggtcag cagcgggatg gccgacgtgg ggtaccagta
    11641 tgtgaacatc gacgattgct ggatgaacgc gtccaagaac cgggattcgc tgcgggtcgg
    11701 gccgctgcgg gacgatcggg gcaacattgt gccgaacaag catttcccgg acatgaaggc
    11761 cttgagcgat tacatccacg gcaaagggct caaggcgggc ctttacactt cacccgggcc
    11821 gctgacctgt gccgggtttg ccggcagcca cgagcacgag gcgcaggatg cgcggcagtt
    11881 tgccgagtgg ggttttgatt ttctcaaata cgactggtgt tcgtacgatg gcgtggtcaa
    11941 gggggatcac agcctggaga cgctgaagcg gccctaccgg ttgatgggcg acctgctcaa
    12001 gcaacagaac cgcgacatcg tcttcaacct ctgccagtac ggcatgggga acgtctggga
    12061 atggggcgaa gaagtcggcg gacattgctg gcggacggcc ggcgatttgg gattcgagtt
    12121 ggatcgcgtc ttcgaggtgg cgctcaagaa cgccgagtac cgcgcccact cgaagcccgg
    12181 aagctggaat gatccggact acatccagat cggactgatt ggcaacgcgc gcggaatggg
    12241 cgagccgatc ccgtgtccgc tcacgcccaa cgagcagtat tcgttcatgt cgctttggtg
    12301 tctgatggcg gcgccgctgt ttttcagtgg cgacatgaac cacctggacg cgttcacagt
    12361 caatgtgctc tgcaaccccg aggtgatcga gatcgatcag gatccgctgg gcgagtgcgc
    12421 ccgtgtgatc ccggcgggcg aggagacgtt cctcatggtg aagaacctcg aagatggcga
    12481 caaagccgtt ggcctcttca atcaaggaga gacggagacc actgtgacgg cccggtggtc
    12541 cgatctcggc gtgaagggac ggcagcccgt gcgcgacctg tggcgccaga agaatctcgg
    12601 acggttccgt aacgcctttg cggcgcgcgt gccggcgcgc ggctgcgtgc tggtgcgttt
    12661 ggactgcggg cgctgatttt gctttccatt tgtcactgaa acgcaatgct gcggcgttgt
    12721 tccgaagact gtttatggac atcaagttgc ccaagctggg tgaaggggcc gattccggcg
    12781 tggtcgtgac cgtgttcgtc aaggaaggcg acacagtcgc gaaagaccaa cccctgctcg
    12841 aactcgaaaa cgaaaaggcc gtcgcgtcga ttccatcgcc ggcggacggc accgtcgcgc
    12901 aagtcttcgt caagccaggc gacaagctca gcatcggtca gcgcatcctc acgctcggcg
    12961 gcgagggagc tccaggcgct tcgaaaggcg tggcctcggc cgccgtggct tcggcccgcg
    13021 gcgtccggtc cgagccggct ccggccaagg ccgccccgcg accgggggcg gcggtcgagc
    13081 ctgaacctga gggtgcccag gaacccggtg cgccggagga ttccgctgat gacaagccgg
    13141 cggcgccgcc cgccgcctcg ccctcgattc ggcggttggc caaggaattg ggcatcgatc
    13201 tgacgcgaat ccgcggcagt cagcgggggg ggcggattgt gatggcagat ttgcgcgcct
    13261 acatcgagcg gctggtgagg ttggccgcgc gcccggctgc caccgccgct cagccgtcaa
    13321 aaccggcggc cgaaccgatc gatttctcga aatggggccc cgtcaccacc aagccgctga
    13381 cgccgctgcg tcagacgatc agccgccgca tggcggagag ttgggcggcc gtcgcgcgcg
    13441 tgacgcaatt cgacgaggtg gatgtgacgc ggttgctggc cctgagaaag cagtatctgg
    13501 ccgcttacga ggagaggggc acgaagctga ccctgaccgg gttcatactc aaggcgctgg
    13561 ccgcggtgct gacgcgccac ccgattttca atgccagcct cgatgcgacc ggcgagaacc
    13621 tggtgttcaa atcgtactat catatcggca tcgcggtcga taccgacgcc ggcctgcttg
    13681 ttcccgtgat ccgggatgcg gacaaaaaaa cgctggttga actctcgcgc gatctcgaga
    13741 ccgtggccat gaaggcccgc gagcgcaaag tgactctgga cgatctgaaa ggcggcacgt
    13801 tcacgttgtc caaccagggt gggattggcg gggcgcactt cacgccgatc atcaacctgc
    13861 ccgaggtggc catcctcgga atcggacggg gggcggtcaa gccggtctgg atcaaggatc
    13921 acgtcgagcc gaggacgctg gtgccgctgg cggtgtcgta cgatcaccgg gtcattgacg
    13981 gcggcaacgc cgcgcggttc atggtcgatc tggtccagac gatcgagggg atgggtgagg
    14041 acgcggtcaa actctagccc gcacgcgaga acggagagga gcctgtcatg gagcgtattc
    14101 aaactgagct ggtcgttgtc ggcgccgggc cgggagggta cacggcggcg ttttacgcgg
    14161 ccgaccgcgg caaacaggtc atgctggtcg aacgcgagcg gcggctgggc ggcgtctgcc
    14221 tcaactgcgg ctgcatcccg tcgaaggccc tgctgcatgc agcgcacacg atcagcttga
    14281 cccgagaatc ggcgcatcgc ggcatgacgt ttgccccgcc gcagcttgac ctgggcaagc
    14341 tgcgcgcctg gaaggaatcg atcctgcagc gtctgggcaa tggtgtgagc cagttggcac
    14401 agaaacgcgg cgtgcgtgtg ctcgagggcc ggggctattt cgaaga
//
LOCUS       DAOQRO010000033        14428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3256_length_14428_cov_5.793015, whole genome shotgun sequence.
ACCESSION   DAOQRO010000033 DAOQRO010000000
VERSION     DAOQRO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14428
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_14428_cov_5.793015"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /gene="dinB"
                     /locus_tag="PKK20_02370"
     CDS             complement(<1..934)
                     /gene="dinB"
                     /locus_tag="PKK20_02370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNU98765.1"
                     /translation="MASYPSMYRVIAHLDMDAFFASIEQRDDPALRGKPVIVGAPPTQ
                     RGVVCAASYEARKFGVRSAMPSATAGRLCPKGVFVRPRMDRYKEESGFILKIMADTGA
                     VIEPMSIDEAYLDLSARCHGEDADASLLSCVPIARQLKERIRSERGLTATVGIASNKL
                     LAKIASDFEKPDGLTVVSERDKVQFLRPLPARVLYGVGRVTEQVLLRVGIRTVGDLQD
                     YSGDLRALVGSFGPKLKQFAVGEDDRPLELGDEVKSISGEETFLQDTEDRGVLRACLR
                     EQAAEIAAKLKRRRLAAHTIQVKVRYGDFTTLTRQ"
     gene            complement(1025..1699)
                     /locus_tag="PKK20_02375"
     CDS             complement(1025..1699)
                     /locus_tag="PKK20_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98766.1"
                     /translation="MGFDDGLWTSTPRQAAPLRAKAATKASSHFRYNAKDLIPEPDAQ
                     QQAVFDQGHGVGALTKELYPDGVEVGENITDLEETVRLTREALRTRKPLFEAALDDQF
                     KVIRRPQHPDVRIGPQCRDPYPCPLQDRCSGFLPLQSVLDLYRGTKKGFGLLDLGVTL
                     LKDIPDDTKLTTSQAIQKATAISGRPHVNHHALAGFLSGLEYPLHFLGFETFGTATSR
                     KLDVSR"
     gene            1795..2499
                     /locus_tag="PKK20_02380"
     CDS             1795..2499
                     /locus_tag="PKK20_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98767.1"
                     /translation="MAPADPLLRLINVTKRFAGVGDASVSVLAGIDLTVERGESLAIV
                     GPSGSGKSTLLSIIGTLDTPSSGTIELEGRNLSQVRGAALAALRNRCIGFVFQAHYLL
                     PQCTVFENVLVPTLATNNEVIRDGAPDRARRLLERVGLGQRLKHRPGQLSGGERQRVA
                     VVRALINEPQLLLADEPTGALDRVSADQLGQMLLDLNRETRVTLIVVTHALSLAGKME
                     RMLELRDGRLDRPRDP"
     gene            2496..6131
                     /locus_tag="PKK20_02385"
     CDS             2496..6131
                     /locus_tag="PKK20_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU98768.1"
                     /translation="MTARRLILRSFAHHVRSHLGALAGTAVASAVLIGALIVGDSVRS
                     SLRQLALLRLGRIETAMSTGDRLCRADLFGSGPGAVASIQHRLGLPSRQVVAAPVLQL
                     PAIAVAGEGAARANQVLVHGVDVRFWKLAEKPFVRGAIPPGSVLLNQPLARQLRVEAG
                     DELLLRVQKPSALSRDAPMATEEDATVALRLRVDAVLSDDQLGRFSLAANQTPPFNAF
                     VSMAELQARVDAVGRANLFVAGSLDTSTQPLPMLPGGGFVPTSVFTNVTAAMNLGLRQ
                     RWRLDDAELELRLAAGSRVAELRSRRVFLDPLLDEAVPAILANTNAARLMPKMESAGL
                     LTYFVNELRVGDRATPYSMVTAAAGPLLPGELADGEILISRWLADDLEAKAGDTLTLA
                     YYVMGTRRDLIEQTAQFRIKGIYEMNTPGIDRSLMPDFPGMTDADNCRDWDTGLPIQN
                     DRIRPKDEEYWQLHRGTPKAFITLATGQKLWANRFGDLTAIRFAVARPSGAAANAPGG
                     LGVTRSSADPIPIANLGSVLSQGLAPGAFGMTFRPVRDEALAASAHSQDFGQLFLGLS
                     FFLIAAALLLVALLFTFGVETRATEVGTLLALGFRPGQVRRLLLWEGGLVAFVGAILG
                     AKGATLYAQAMLKGLSTVWNQAVGGATLSFHLHTPTILLGVAASILVTVVAMWFALRH
                     QAAQPARALLAGGAQWDQDSNNFGDPVSGAQCPTQRQAESPMSDAGGSLTPVVSRISA
                     PGQLQVICALAATALALGLAFWGWQRGEKQSAGLFFAAGALLLVAGLCACAGWLVRLA
                     ATSAKTRLTRSSLAVRGATRRRRRSLAVIGLLASGSFLVAAIGVFRLDAVQNAQQRSS
                     GTGGFALIGQSTQPVLHDLESERAREVYGLDAARLEGMSVVPFRVREGDEASCLNLNR
                     AQEPRILGVAPGLLAKRSAFTFAQAAKGLSRFEGWNLLVSRTRADSDGAAVDGISDDD
                     TVPAIGDQASVVWALGKKVGDTIPLVDERGRPFRIRLVAAVANSILQGNLIIAENEFV
                     KRFPGVAGYRMFLIDTPSNRIDQVSSELTRGLQDLGLELTPASERLAAFNAVQNTYLG
                     TFQVLGGLGLLLGSLGLGVVVLRNVLERRGEYALLLAVGWERSPLRRLVIIEHGVLLA
                     AGLAVGVCAAFVAVLPSVLGPGTEVPYWTLAWTLAGVLLNGFVWTWLATLTALRGNAL
                     EMLRNNQ"
     gene            6260..6961
                     /locus_tag="PKK20_02390"
     CDS             6260..6961
                     /locus_tag="PKK20_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98769.1"
                     /translation="MILETRLLGLLFILAFIGGSPHARAGESAVLYRNDFTKAEIGTL
                     PDGMLALNGKFAVREEAGNRFLELPGTPLDTYGVLFGPAGREDGAGSHSSDQPAEIDN
                     LSVTARIHGTNTGRRYPAFAVGLGGVSGYRLQVSPGKRKLELCRGDADHVSVPFEWAP
                     GEWLWLKLEVRKTNPTEWTIRGKVWKEGTAPPSSWTISRTDTTSLKPGRASLWGSPFA
                     DTPIRFDDVSVERIR"
     gene            complement(6958..10092)
                     /locus_tag="PKK20_02395"
     CDS             complement(6958..10092)
                     /locus_tag="PKK20_02395"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNU98770.1"
                     /translation="MIAAREELVKPPASPSARRRSARPSPSRFVSDRRVAPRRLRIER
                     FFSDPKVKPFDQIEWEKRTAEITDDTRKVIFNQEGVEVPKAWSPLATKVVCSKYFYGD
                     PQKSEEREYSVKQLIHRVTRTIADWGVKDGYFSAADGEVFYDELTWLCVNQYGAFNSP
                     VWFNVGLYQQYHVGKESARGNWYYHRTRGEAERAATQYEYPQGSACFIQNVEDNMESI
                     MHLAYSEAMLFKYGSGTGTDLTPIRSSKEKLSGGGRPSGPLSFLKVYDQVANVVKSGG
                     KTRRAAKMNTLRDWHGDIEEFIEAKEKEERKAWALIEQGYDGSFNGNAYASVMYQNEN
                     LSVRVSDEFMRAAIEGREWWTRSVTTGEPLEKKDAGLLLDKIAGGTWICGDPGLQYDG
                     AIQKWHTCKGTEPIHSTNPCSEYVFLNNTACNLASLNLLKFKREDGAFDVERFKAAVR
                     IFIIAQEILVDNSSYPTKAIAENSHIFRSLGLGYANLGSLIMDEGLPYDSDEARALAG
                     AVTAIMTGHAYEQSAEIAAIMGAFDGYRDARCAGVSKPSAPDNVESMLGVIEQHRAAV
                     EDIHPSEKYGYLKESARACWVRALERGREVGYRNAQVTVLAPTGTIAFLMDCDTTGIE
                     PDIALVKYKLLAGGGTLKIVNRTVPGGLRSLGYGPEEIQKIVAHIEQYDTIEDVTVEA
                     SGEQGPRVVRSGLKPEHLCVFDCACKPNRGGRSILALAHLSMMAAAQPFISGAISKTV
                     NMPCETTVAEIRDAYVQAWKMGLKCVAIYRDGSKRCQPLNTRKTTAEGEAKTASGAPA
                     DGWEARVKELEAEVEKLRTELRRPPRRRLPETRMAVNHKFNIAGHEGYLNVGLFENNQ
                     PGELFITMAKEGSTIGGLMDTIATLTSIALQYGVPLATLVKKFSHQRFEPSGITKNPD
                     IRFASSITDYIFRWLAVQFIPGYREETSPDRNQQELPMPGLIEEIKKRVNRPVPYLPI
                     DDEEAASLAKPSEIAHVVTSTPTPGPIAYAQTTTATFVNMADAPLCPNCGHVTVRAGA
                     CFKCNNCGESLGCS"
     gene            complement(10383..11543)
                     /locus_tag="PKK20_02400"
     CDS             complement(10383..11543)
                     /locus_tag="PKK20_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98771.1"
                     /translation="MNVFNGIIILAAFGAVSADRSLAGQVDWDRPAAAAYLDDRVDLW
                     IDRATQLQTGESKLACVSCHTVVPYLLVRPALRKAMQTVKPTSQEARLLDNVVRRVET
                     CWNRGSITDHENAGHDGTEEVLNALILARRDAAEEKQQASDATRAAFRRLWETQRSDG
                     AWDWMDFAQEPDESAAARFYGAALAALAVGTVPPLSGSSEGYSAYREGNLRGYLRQNH
                     RQQNLYNRVWLSLASARWPGLLTWTEQEELIAELRRKQNRDGGWSLYGLGPWRWSKTS
                     PPFEPSEKLDWPLLEQSDGYATGLIIYALLEARVPPTDAALVRAARWLRSNQQAILVD
                     GYRWKCWQAPSLNRVYEPGSSHGGTWERMMMSDLATAFAALALVTLDQEPGR"
     gene            complement(11852..12601)
                     /locus_tag="PKK20_02405"
     CDS             complement(11852..12601)
                     /locus_tag="PKK20_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HNU98772.1"
                     /translation="MSPYLAEFIGTAILVIFGNGVVANVVLNRTKGSGGGWIVIAAGW
                     GLAVFVGVFCSAPFSGGHLNPAVTVAMVAAGKLAGGQVAGYLLAQMLGGVLGGNLVFL
                     FYREHFKATDNADLKLASFCTAPNIRRLPQAFFCETLGTFLLVLPVFLMTDPTVKIPS
                     AAGAVAEIPVGLGTLGALPVALLVFGIGLSLGGTTGYAINPARDLGPRIAHALLPVPG
                     KRDSDWNYAWVPVLGPLAGGLLAAMVNAWLR"
     gene            complement(12598..14109)
                     /gene="glpK"
                     /locus_tag="PKK20_02410"
     CDS             complement(12598..14109)
                     /gene="glpK"
                     /locus_tag="PKK20_02410"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNU98773.1"
                     /translation="MKAILALDQGTTSSRAIVFDHDGSPRGLAQKEFKQSYPQAGWVE
                     HDPREIWESQLATAREALAQAGLEAGDIAAIGITNQRETTVVWDRRTGEPVHPAIVWQ
                     DRRTARFCDDLKRAGHETVIQAKAGLVIDAYFSGSKLRWILDRVPGARERAARGDLAF
                     GTIDTWLLWRMTNGALHLTDPSNASRTMLYNLDTGDWDDELLGILGVPREVLPHVRSS
                     SEVYGETADGVFGAPIPIAGIAGDQQAALFGQNCFESGQAKNTYGTGCFMLMNVGTKP
                     VASRHRLVSTVAWQAGGRTDFALEGSVFIGGAVVQWLRDGLGLIKSSAEIERLAATVP
                     DCGDVYLVPAFAGLGAPHWDQYARGTITGLTRGTTAGHLARAALESIAFQVADVLDVM
                     RQDSGVPIHELRADGGACANDLLMQFQSDILRARVARPKVIETTALGAAYLAGLAVGF
                     WKDRAEVERAWQIDRVFEPRMSADEAAHRRDRWTKALRRALDWEEHSMRDVNT"
     gene            complement(14112..>14428)
                     /locus_tag="PKK20_02415"
     CDS             complement(14112..>14428)
                     /locus_tag="PKK20_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNU98774.1"
                     /translation="HRARTTMDSPGPPRILIHSLVVTNGHVGFADLTRKTPFRTAYAP
                     INLEIAGSIDPVEDRDALARGLVQGLVKAARLGELSAIGRSPPDADASFRGQTRVTLS
                     PN"
BASE COUNT         2422 a         4751 c         4630 g         2625 t
ORIGIN      
        1 gttggcgagt cagggtcgtg aagtcgccgt accgtacctt gacctgaatc gtgtgtgcgg
       61 ccaggcgacg ccgcttgagc ttggccgcga tctccgccgc ttgctcccgc agacacgccc
      121 ggagtacgcc ccggtcctcg gtgtcctgca agaacgtctc ctccccactg atgcttttga
      181 cctcgtcgcc gagctccagc ggacggtcat cctcacccac cgcgaactgc ttcagcttcg
      241 ggccaaatga cccgaccagc gccctcaggt cgccgctgta atcctgtaag tcgccaaccg
      301 tccggatgcc aactcgcagc agcacctgct ccgtgactcg gcccacgcca tacaggacgc
      361 gcgccggcag cggacggagg aactggacct tgtcccgttc ggaaaccacg gtgagcccgt
      421 ccggcttctc gaagtcgctg gcgatcttgg ccaggagctt gttggaggcg atgccgacag
      481 tcgccgtcaa gccgcgttcc gaccggatgc gctccttcaa ctggcgggcg atgggcacgc
      541 acgagagcag ggaggcgtcg gcgtcctccc catgacagcg cgcggacaag tcgaggtacg
      601 cctcgtcgat ggacatcggt tcgatcaccg cgcccgtatc ggccatgatc ttgagaatga
      661 agccggactc ctccttgtat cggtccattc tggggcggac gaagacgcct ttggggcaga
      721 ggcgaccggc ggtggcgctg ggcatggcgg agcggacgcc gaacttgcgc gcctcgtagc
      781 tggcggcgca gacgacacct cgctgtgtgg gcggggcgcc gacgatgacc ggcttaccgc
      841 gcaaggccgg atcatcgcgc tgctcgatcg aggcgaaaaa cgcgtccata tccagatggg
      901 cgatcacgcg gtacatcgaa gggtaggatg ccatttccgg gacgatgaac caaggcttgg
      961 acttccgcgt gagatgcggc ctccgcaaga tgctgcctgg ggaacacgag acgcgcagaa
     1021 acgctcaacg tgaaacgtct aacttccggc tcgtcgccgt gccgaaggtc tcaaaaccca
     1081 agaagtgtag cgggtattcc agcccgctca ggaatcccgc gagcgcgtga tggttgacgt
     1141 gcggtcggcc ggagatggcg gtcgccttct gaatcgcctg actggtcgtt aacttggtgt
     1201 cgtcgggaat gtccttcagc aacgtcacac ccaggtcgag cagaccgaag cccttcttcg
     1261 tgccacggta cagatcgagc acgctctgta ggggcagaaa acccgaacac cggtcctgga
     1321 gcggacaggg gtagggatcg cggcactggg ggccgatccg aacgtcgggg tgttggggtc
     1381 gccggatgac cttgaactgg tcgtcgagcg cagcctcgaa cagcggcttg cgggttcgga
     1441 gcgcctctcg cgtcaggcgg acggtctcct ccaaatctgt gatgttctcg ccgacctcga
     1501 ccccgtccgg gtagagctct ttggtcaacg cgcccactcc gtgcccctga tcgaagaccg
     1561 cctgctgctg ggcatccggc tctgggatca ggtccttggc attgtagcgg aagtgagaag
     1621 aggctttggt tgcggctttg gcgcgcagcg gcgccgcttg gcgaggcgtc gaagtccaca
     1681 aaccgtcgtc gaagcccaac aagcgcgaat gggtgacgcc cctcgtcggt ctgaggcgga
     1741 ggctcgctga aactgggttg aatctcgaac gcgctcaact accttgagtc ccgtatggcg
     1801 cctgccgatc cattgctcag actgatcaac gtcaccaagc gctttgccgg cgtcggcgac
     1861 gcatcggtgt cggtcctggc cggcatcgac ctgacggtcg agcggggcga atcgctagcg
     1921 atcgtcgggc cgtccggctc gggcaagagc accctgttga gcatcatcgg cacgctcgac
     1981 accccctcgt cgggaaccat cgaactcgag ggacggaatc tgagccaggt gcggggcgcc
     2041 gcgctggcgg ccctgcgcaa ccggtgcatc ggattcgtct ttcaggcgca ttacctcctg
     2101 ccgcaatgca ccgtattcga gaatgtgctg gtgccgaccc tcgccaccaa caacgaggtc
     2161 atccgcgatg gcgcgccgga ccgcgcccgg cgcctgctcg aacgcgtggg cctgggccag
     2221 cgcctcaaac atcgcccggg acaattgtcc gggggcgaac gccagcgggt ggcggttgtg
     2281 cgggcgctga tcaacgagcc tcagctcctg ctggcggacg agccgaccgg cgccctggac
     2341 cgggtttcgg ccgatcaact gggccagatg ctgctcgatc tgaaccgcga aacgcgcgtg
     2401 actctgatcg ttgtcaccca cgccctgagc ctggccggca agatggagcg tatgctcgaa
     2461 ttgcgcgacg ggcggctgga ccgcccgcgt gacccatgac cgcgcgtcga ctcattctcc
     2521 gcagtttcgc ccaccacgtc cgctctcacc tcggcgcgct ggcaggcact gccgtcgcat
     2581 ccgcggttct catcggcgca ttgatcgtcg gcgattccgt ccgctcgagt cttcggcaac
     2641 tggcgctgct gcgcctgggc cggatcgaga cggccatgtc cacgggcgac cggctttgtc
     2701 gcgccgacct gttcgggtcg gggccgggcg cggtcgcgtc catccagcac cgcctcggcc
     2761 tgcccagccg gcaggtggtc gccgcgccgg tcctgcaact tccggccatt gcggtcgcgg
     2821 gcgaaggcgc ggcgcgcgcc aatcaggtct tggtgcatgg cgtcgatgtc cggttttgga
     2881 agttggcgga gaaaccgttc gttcgcggtg ccataccccc cggcagcgtg ctgctcaacc
     2941 aacccttggc ccgccaactg cgcgtggaag cgggcgacga gctgcttctg cgcgtccaga
     3001 aaccctcggc cctctcgcgc gacgccccca tggcgaccga agaggacgcg accgtcgccc
     3061 tgcgtctccg ggtcgacgcg gttctctcgg acgaccagtt agggcggttc agtctcgccg
     3121 caaaccagac gcctcctttc aacgcgtttg tgtcaatggc cgaactccag gcgcgcgtcg
     3181 atgccgttgg ccgggcgaat ctgttcgtgg ccggatcgct cgacacctcg acgcagcctc
     3241 tgcccatgct gccgggcggc ggattcgtgc ccaccagcgt cttcaccaat gtcactgccg
     3301 ccatgaacct cggactccgc caacgctggc gcctggacga cgccgagttg gagttgcggt
     3361 tggcagcggg gagccgagtc gccgaattgc gcagccgccg ggtcttcctc gatccgcttc
     3421 tggacgaggc tgtgcctgcg attctcgcga acaccaacgc ggcccggttg atgccgaaga
     3481 tggaatcggc cggtttgctc acctacttcg tcaatgaact tcgggtcgga gaccgcgcca
     3541 cgccgtattc gatggtcacc gcggcggcag ggccactgct cccgggcgag ttggctgacg
     3601 gcgagattct gatcagtcga tggctggccg acgatctcga agccaaggcc ggcgacacgc
     3661 tgacactggc gtactacgtc atgggcaccc ggcgtgacct gatcgagcaa accgcccagt
     3721 tccggatcaa gggcatctac gagatgaaca cgcccggcat cgatcgctcg ctcatgcccg
     3781 atttccccgg catgaccgat gccgacaact gccgggattg ggacactggc ctgcccatcc
     3841 agaacgaccg catccgtccc aaggacgagg aatactggca attgcaccgc ggcacgccaa
     3901 aagcctttat caccctggca accggacaga agctctgggc gaatcgcttt ggcgacctga
     3961 ctgcaattcg cttcgcggtg gcccgcccga gcggcgcggc cgccaacgca cctggtgggc
     4021 tcggcgtcac ccgatcgagc gccgatccga tcccgatcgc caacctcggt tctgtcctgt
     4081 ctcaagggct ggcgcccggc gcgttcggga tgacgttccg tcccgtgcgc gacgaggcgt
     4141 tggcggccag cgctcactcg caggatttcg gtcaactgtt cctcggtttg agcttcttcc
     4201 tcatcgcggc ggcgctgctg ctggtggcat tgctcttcac gttcggcgtc gagacgcgcg
     4261 caacggaagt cggaaccctg ctcgcactcg gtttccggcc cggccaggtg cggcgtctgc
     4321 ttttgtggga aggcggcctg gtggcgttcg tgggcgcgat cctcggcgcg aagggggcaa
     4381 cgctctacgc ccaggccatg ctgaagggcc tctcgaccgt ctggaaccag gcggtaggcg
     4441 gcgccacgct gagctttcat cttcacacgc ccacgattct gctgggcgtg gcggcatcga
     4501 tcctggtcac ggtcgtggcc atgtggttcg ccctcagaca ccaggccgcc cagcccgcac
     4561 gcgcccttct ggccggcggc gcgcaatggg atcaggactc gaacaacttc ggcgacccgg
     4621 tgtccggcgc gcaatgcccg acgcaacgac aggctgagag tccgatgtca gacgccggag
     4681 gttctctcac gcctgttgtg tcccggatca gcgccccagg acaactgcaa gtcatctgcg
     4741 cccttgccgc gaccgccctg gcccttggac tcgccttctg gggctggcag cggggtgaaa
     4801 agcagtcagc cggactgttc tttgcagcgg gcgcgttgct gttggttgca gggctgtgcg
     4861 cctgcgccgg ctggctggta cgcctggcag ccaccagcgc caaaaccagg ctgacccgat
     4921 cgagcctcgc ggtgcgcggc gcaacgcggc gccggcgccg cagcctggca gtcatcggcc
     4981 tgctggcttc cggcagcttc ctcgtcgcgg ccatcggcgt cttccgcctc gatgccgtcc
     5041 agaacgctca gcagcggtct tcaggcacgg gcggctttgc actcatcggc caatcgaccc
     5101 agccggtcct ccatgacctc gagagcgagc gggcgcgcga ggtctatgga ctggacgcag
     5161 cccgtctcga aggcatgtcg gtagttccgt tccgggtgcg cgagggcgac gaggccagtt
     5221 gcctgaatct gaatcgcgcc caggaaccga ggattctcgg cgtcgcaccc ggcttgttgg
     5281 ccaaacgcag cgccttcacg ttcgcccaag cggcgaaggg attgtctcga ttcgaagggt
     5341 ggaacctgct cgtgtccaga acgcgcgccg actccgacgg agccgcggtc gatgggattt
     5401 cggacgatga cacggttccc gcgatcggcg accaggcctc ggtcgtctgg gccctgggca
     5461 agaaggtcgg cgacacgatc ccgctcgtgg acgaacgggg tcggccgttt cgaatccggc
     5521 ttgtggctgc ggtcgccaac tcgatcctgc aagggaacct gatcatcgcc gagaacgagt
     5581 tcgtgaaacg cttcccgggc gtggcggggt accgcatgtt cctgatcgac accccctcga
     5641 accgcatcga ccaggtctcc tccgaactca cccgggggtt gcaggatctg ggattggaac
     5701 tgacgccggc cagcgaacgg ctggcggcgt tcaacgccgt gcagaacact tacctcggga
     5761 cgttccaggt gttgggcgga ctcgggctgc tcctgggcag cctcgggcta ggcgtggtgg
     5821 tgttgcgcaa cgtgctcgaa cggcgcggcg agtatgccct cctcctggcg gtggggtggg
     5881 aacgcagccc cttgcgccga ctcgtgatca tcgagcacgg ggtcctgctg gccgccggcc
     5941 tcgccgtcgg cgtctgcgcc gcctttgtgg cggtcctgcc ttcggtgctc ggccccggca
     6001 ccgaggtgcc ctactggaca ctggcctgga cactggctgg ggtgctcctc aacggctttg
     6061 tctggacctg gctggcgaca ctgaccgctc ttcgcggcaa cgcgctcgaa atgctgcgaa
     6121 acaaccaatg attccgctgg agactgcgcc tatgacaaaa ctcggacgtg accatcgacg
     6181 cgttgttgcc gcccgcccgc ccggacgccg gcctggccca tttgcagcac agcccgccgg
     6241 tctcggtcca tgccgcgtca tgatcctcga gacgcggctc ttgggactgc tgttcatcct
     6301 ggctttcatc ggtggctctc cccatgcccg cgccggagag tccgccgtgc tctatcgaaa
     6361 cgacttcacc aaggccgaaa tcggcacgct gcccgacgga atgctggcgc tgaacgggaa
     6421 gttcgccgtg cgcgaggagg cgggcaacag gttcctcgag ctgccgggga cgccgctcga
     6481 cacgtatggc gttctctttg gcccggccgg aagggaggat ggcgcggggt cgcacagttc
     6541 ggatcagcct gccgaaatcg acaacctgag cgtgaccgcg cggattcatg gcaccaacac
     6601 cggacggcgt tacccggcat tcgcggtcgg gctgggaggc gtgagcggct atcggctgca
     6661 ggtgagccca ggcaagagga aactcgaact gtgtcgcggc gatgcggacc acgtcagcgt
     6721 gccgttcgag tgggctccgg gcgagtggct ctggctgaag ctcgaagtgc gcaagacaaa
     6781 tccaaccgaa tggacaatcc gcggcaaggt ctggaaagaa ggcacagccc ctccgtcgag
     6841 ctggacgatc tcgcggacgg acaccacgtc cctcaagccg ggccgcgcca gcctctgggg
     6901 ctcgcccttc gccgataccc cgatccgctt cgatgacgtg tcggtggaac ggatacgcta
     6961 gctgcaccca aggctctcgc cgcaattgtt gcacttgaaa caggccccgg cgcgcacggt
     7021 gacatgcccg caattcgggc acaggggcgc gtcggccatg ttgacgaacg tggccgttgt
     7081 ggtctgtgcg tacgcaatcg gacctggcgt cggcgtcgag gtgacgacat gggcgatctc
     7141 cgatggcttg gccagggatg ccgcctcttc atcatcgatc gggaggtagg ggacgggcct
     7201 gttgacgcgc ttcttgattt cctcgatgag gccgggcatc ggcagctcct gctggttgcg
     7261 gtcgggcgag gtctcttccc gatagcccgg gatgaactgg acggcgagcc agcggaagat
     7321 gtagtcggtg atcgagctgg cgaagcggat atcggggttc ttggttatgc cgctgggctc
     7381 gaaccgctgg tggctgaact tcttgaccag cgtcgcgagc ggcacgccgt actgcagggc
     7441 gatgctggtg agggtggcaa tcgtgtccat gaggccgccg atggtcgacc cttccttggc
     7501 catggtgatg aacagttccc caggctggtt gttctcgaac aacccgacgt tgagatagcc
     7561 ctcgtgtccg gcgatgttga acttgtggtt caccgccatg cgcgtctcgg gcagccgccg
     7621 ccggggcggg cgccgcagct cggtccggag tttctccacc tcggcttcga gttccttgac
     7681 ccgggcctcc cagccatcgg ctggagctcc ggacgcggtc ttggcctcgc cttcggcagt
     7741 cgtcttcctg gtgttcagcg gctgacagcg cttcgagccg tcgcggtaga tggcgacgca
     7801 tttgaggccc atcttccagg cctggacgta ggcatcgcgg atctcggcca cggtggtttc
     7861 acacggcatg ttgaccgtct tggaaatggc gccgctgatg aagggctggg cggcggccat
     7921 catgctcaaa tgcgcgaggg cgagaatgct gcgcccgccg cggttgggct tgcacgcgca
     7981 gtcgaacacg cacaggtgct cgggcttgag accgctgcgg acgactcgcg gcccctgttc
     8041 tccagacgcc tcgaccgtca cgtcctcgat cgtgtcgtac tgctcgatgt gcgcgacgat
     8101 cttctgaatc tcctccgggc cgtacccgag cgaacgcagg ccgccgggga ccgtgcggtt
     8161 gacgatcttg agcgtgccgc cgccggccag cagcttgtac ttcaccagcg caatgtccgg
     8221 ctcgatcccg gtcgtgtcgc agtccatgag aaacgcgatt gtgcccgtcg gcgcaagaac
     8281 ggtgacctga gcgttgcgat agcccacctc gcggccccgc tccagggcgc ggacccaaca
     8341 ggcgcgggca gactctttca ggtacccgta cttttcgctg ggatgaatgt cctcgaccgc
     8401 ggcgcgatgc tgctcgatga cgccgagcat tgactcgacg ttgtcgggtg cgctgggctt
     8461 ggagacgccc gcgcagcggg cgtcgcggta cccgtcaaag gcgcccatga tggcggcgat
     8521 ctcggcgctc tgctcatacg cgtggccggt catgatcgcc gtgacggcgc ccgccagcgc
     8581 ccgcgcctcg tccgagtcgt agggcaatcc ctcgtccatg atgagcgagc ccaggttcgc
     8641 gtagccgagg ccgagcgagc ggaagatgtg ggagttttcg gcgatggcct tggtggggta
     8701 gctcgaattg tcgacgagga tttcctgcgc gatgatgaaa atgcgcacgg cggccttgaa
     8761 acgctcgacg tcgaacgcgc cgtcctcgcg cttgaacttg agcaggttca gcgaggcgag
     8821 attgcaggcc gtgttgttga ggaatacata ctcgctgcac ggattggtcg agtggatcgg
     8881 ctcggtcccc ttgcacgtgt gccatttctg gatcgcgccg tcatactgga ggccgggatc
     8941 gccgcaaatc cacgtgcccc cggcaatctt gtccaataag aggcccgcgt ccttcttctc
     9001 gagcggctcg cccgtcgtga cggaccgcgt ccaccactcc cgcccctcga tcgccgcccg
     9061 catgaactcg tcgctgacac ggactgacag attctcgttc tggtacatga ccgacgcgta
     9121 ggcgttgcca ttgaacgaac cgtcgtagcc ttgctcgatc agcgcccagg ccttccgctc
     9181 ctccttctcc ttggcttcga tgaactcctc gatgtccccg tgccagtccc gcagcgtgtt
     9241 catcttcgcc gcccgacggg tcttgccgcc cgacttcacc acgttggcaa cctggtcgta
     9301 aaccttgagg aacgacagtg gcccgctcgg ccgccccccc ccgctcagct tctccttgct
     9361 cgaccggatg ggcgtcagat cggtccccgt ccccgatccg tatttgaaaa gcatcgcctc
     9421 gctgtaggcc agatgcatga tgctctccat gttgtcctcg acgttctgaa tgaaacaggc
     9481 gctgccctgc ggatactcgt actgcgtcgc cgcgcgctcg gcctcgccgc gcgtgcgatg
     9541 gtagtaccag ttcccgcgcg cggattcctt gccaacatga tactgctggt acaaacccac
     9601 gttgaaccag acaggcgagt tgaaggcgcc atactggttc acacacaacc acgtcaattc
     9661 gtcgtaaaac acctccccgt ccgccgcgct gaaataaccg tccttcacac cccagtcggc
     9721 aatcgtccgt gtcacccgat ggatcaactg cttgaccgaa tactcgcgtt cctccgactt
     9781 ctgcggatcg ccgtaaaagt acttcgagca gaccaccttc gtggccaaag gcgaccacgc
     9841 cttgggcacc tcgaccccct cctggttgaa gatcaccttg cgcgtgtcat ccgtgatctc
     9901 cgccgtccgc ttctcccact cgatttgatc gaacggcttg accttcggat cgctgaaaaa
     9961 acgctcgatc cgcagccgcc tcggcgcaac gcgcctatcg gacacgaaac gtgacgggga
    10021 gggacgcgcg ctgcgccggc gcgcggacgg cgaggccggg ggcttaacaa gttcttcgcg
    10081 ggcggcaatc atggctattg gctggttcga tttgatcggt gaaagtctac aactcgggct
    10141 gcgccgggac aacgcccgac acctttccca aagagcaaaa ttgcccgcca aaccaagggc
    10201 tttgcgatgc acaggcaaag caagaaaccc catttagttg ggcaagctgg gagaagctaa
    10261 tacaatgggt tgtggttgca aggaaaatcg atggctttcg caacttttct gttacatcct
    10321 cgatccgaac gggttcccga cccgtttact cgtcaagtcg agaccacggc ttcccgaacg
    10381 actcaacgcc caggttcttg gtcgagcgtg acaagggcca aggcggcgaa cgcggttgcc
    10441 aggtcggaca tcatcattcg ctcccaggtg ccgccgtggg agcttccggg ctcgtaaacg
    10501 cggttgaggg agggcgcttg ccagcatttc cagcgatacc cgtccaccag gatcgcctgc
    10561 tgattgctcc tgagccatcg tgccgctcgt acgagagccg catcggtggg cggcacgcgc
    10621 gcctcgagca gcgcgtaaat gatcaaaccc gttgcatagc catccgattg ctccagcagc
    10681 ggccaatcga gcttttcgga gggctcgaag ggtggactgg tcttggacca ccgccacggt
    10741 cccaaaccat agagcgacca acctccgtcc cgattctgtt tccgccgcag ttcggcgatc
    10801 agttcttcct gctctgtcca ggtgagcagg cctggccagc gcgccgacgc caacgacagc
    10861 cagacccgat tgtagaggtt ttgctggcga tggttttgac ggagataacc acgcagattc
    10921 ccctcgcggt atgcagaata gccctcgctt gagcccgaaa gcggaggcac ggtgcccacc
    10981 gcgagagccg ccagggcagc cccgtaaaac cgcgctgcgg cagattcatc cggctcctgc
    11041 gcgaaatcca tccaatccca ggctccgtcg gagcgctgtg tttcccagag ccggcgaaag
    11101 gccgcgcgtg tggcatcgct ggcttgctgt ttctcctcag cggcatcccg tcgggccagg
    11161 atgagcgcgt tcaaaacctc ctccgtcccg tcgtgacccg cgttctcgtg atcggttatg
    11221 gagccacgat tccagcaggt ctcgactcgg cggacgacgt tgtcgaggag ccgggcctcc
    11281 tgcgaagttg gcttgacggt ctgcatcgcc ttgcgcaggg cgggacgaac caacaaataa
    11341 gggaccaccg tgtggcatga aacgcaggcc agcttgcttt cgccggtttg gagttgtgtg
    11401 gcgcggtcga tccagagatc gactcgatca tccaggtagg cggccgctgc cggccggtcc
    11461 caatcgacct ggccggcgag actgcggtcg gcgctgacgg cgccaaacgc ggccaggatg
    11521 ataatcccgt tgaagacatt catcgagtgc tggctccacc tttagaccgg catggccgtt
    11581 ccagcaagcc cggacgtttc gcaccgctgc ccgcaatcgg gtgtccgact ccgcgaagct
    11641 ttgtgtcgcc atctgtacac ttctccattg gctgcacgtg atctgcacgc cgctcctgac
    11701 gttcttcgcg gttcacctcg cgcgcagacc ctgagaaaca gaacctcctg gatcgcctga
    11761 ttcgatcagc cctccggcgt tgggcgacag cgttgtcgta ccgggagcgt ccgcgaactc
    11821 ctgggaactg cgcgacacct tgattttcgc gtcagcgcag ccaggcgttg accatcgccg
    11881 cgagcaggcc cccggcgagc ggtccgagga cggggaccca tgcgtaattc caatcgctgt
    11941 cccgcttgcc tggcaccggc aacagagcgt gggcaatgcg cgggccgaga tcgcgtgccg
    12001 gattgatggc atagccggtc gtgccgccca aggacaaacc gatgccgaac acgagcaggg
    12061 caaccggaag cgcgcccaat gtgccgaggc caacgggaat ctcggccacg gctccggcag
    12121 ccgacggaat cttcaccgtc ggatcggtca tcagaaacac cggcaggacc agcaggaatg
    12181 tcccgagagt ctcgcagaag aaggcctgag gcaggcgccg aatattcggc gcggtgcaaa
    12241 aggaggccag cttgaggtcg gcgttgtccg tggctttgaa gtgctcgcgg tagaacagaa
    12301 acaccagatt cccacccagc acgcccccca gcatctgagc aaggagataa ccggccacct
    12361 ggccacccgc cagcttcccg gccgcgacca tcgccaccgt gacggcggga ttgagatgcc
    12421 cgccgctaaa cggcgcggag cagaacacgc cgacgaacac cgcgagcccc cagcccgccg
    12481 cgatcacaat ccagccgccg ccgcttccct tggtgcgatt gagcacgacg ttggcgacga
    12541 cgccgttgcc gaagatgacc agaatagccg ttccgataaa ctccgcgaga taagggctca
    12601 tgtgttgaca tctctcatcg agtgctcttc ccaatccagg gcgcgccgca gcgccttggt
    12661 ccaacggtcg cgtcggtgcg ccgcctcgtc ggcgctcatc cgcggttcga atacccggtc
    12721 gatctgccag gcgcgttcga cttccgcacg gtctttccag aaaccgacgg caaggccggc
    12781 gaggtacgcg gcgcccaggg cggtggtctc gatcactttg ggccgcgcca cccgcgcgcg
    12841 caggatgtct gactggaact gcatcagcag gtcgttggcg catgccccac cgtcggcccg
    12901 caactcatgg atcgggacgc cggagtcctg gcgcatgaca tcgaggacat cggcgacctg
    12961 gaaggcaatg ctttcgagcg ccgcgcgcgc aaggtgcccg gcggtcgtgc cgcgtgtcag
    13021 gcccgtgatc gtgccgcgcg cgtattggtc ccagtggggc gcgcccaggc cggcgaacgc
    13081 cgggaccagg tacacatcgc cgcagtccgg cacggtcgcg gcaagccgct cgatctcggc
    13141 cgaggacttg atcaggccca gtccatcgcg cagccattgc accacggcgc ccccgatgaa
    13201 gacactgcct tcgagggcga aatcggtccg gccgcccgcc tgccaggcca ccgtgctgac
    13261 gagccgatgt cgcgaggcga cgggttttgt gccgacgttc atgagcatga aacaaccggt
    13321 gccgtaggtg ttcttggcct ggccgctctc gaagcagttc tggccgaaga gcgccgcttg
    13381 ctggtcgccc gcgatgccgg cgatcgggat gggggccccg aacacgccat ccgcggtctc
    13441 gccgtacact tcgctcgaag accggacgtg gggcagcacc tcgcgcggca ctcccaggat
    13501 gccaagcaat tcgtcgtccc agtcgcctgt atcgaggttg tagagcattg tccgcgacgc
    13561 gttgctcggg tcggtcaggt gaagcgcgcc gttggtcata cgccacaaca accaggtgtc
    13621 gatcgttcca aacgccagat cgccccgggc cgcgcgctct cgggcgccgg ggacccgatc
    13681 gagtatccag cgcagtttgc tccccgagaa ataggcgtcg atgaccagcc cggcctttgc
    13741 ctggatgacc gtctcgtggc ccgcacgctt gaggtcgtcg cagaatcggg ccgtgcgccg
    13801 gtcctgccac acgatcgcag ggtgaacagg ctcgcccgtc cggcgatccc agacgaccgt
    13861 cgtctcacgc tgattggtga tgccgatggc agcgatgtcg ccggcctcta gccccgcctg
    13921 ggcgagggcc tcgcgcgcgg tcgcgagctg cgattcccaa atctcccgcg ggtcgtgctc
    13981 gacccatccg gcttgcgggt aagactgttt gaactctttc tgggcaaggc cgcgtgggct
    14041 tccgtcgtgg tcgaaaacaa tcgcgcgcga actggtggtg ccctgatcga gggcgagaat
    14101 ggctttcatg gtcaatttgg cgagagcgtg acacgggtct ggccccggaa cgaggcgtcc
    14161 gcgtcgggcg ggctccgtcc gatggccgac aactcaccga ggcgtgccgc cttgaccagg
    14221 ccttgcacca ggccgcgcgc cagtgcgtcg cggtcctcga ccggatcgat cgagccggcg
    14281 atttcgaggt tgatcggcgc atacgcggtg cgaaacggtg tcttgcgtgt cagatcggca
    14341 aaaccgacgt ggccgttggt cacgacgaga ctgtgaatga gaatccgggg tgggccaggc
    14401 gaatccatcg tagttctggc gcggtgtt
//
LOCUS       DAOQRO010000034        14390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3264_length_14390_cov_4.931915, whole genome shotgun sequence.
ACCESSION   DAOQRO010000034 DAOQRO010000000
VERSION     DAOQRO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_14390_cov_4.931915"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKK20_02420"
     CDS             <1..311
                     /locus_tag="PKK20_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HNU98775.1"
                     /translation="EIEVLELAAQPVHPDDAHTVWNAADVLDVLDRNRNIVAWFNGHN
                     HAGAFAVRNGVPFVTIAGMVETRDANAYAVLNLCAKHFELVGHGRQTSRTAPFGPDVS
                     "
     gene            complement(355..1626)
                     /locus_tag="PKK20_02425"
     CDS             complement(355..1626)
                     /locus_tag="PKK20_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNU98776.1"
                     /translation="MKRTNRLSNGAQTTLLLLLELLLAPGIHAVDWPQWFGSDRTGRS
                     AETGLLKTWPADGPRLSWKTAGLGSGFTTVSVAQGRIFTAGDMADGNYIIALKESDGT
                     KLWTSRLGKAGAPGWGGFAGPRCTPTVDGDRLFAVGQYGELACFETATGKELWRKHLV
                     TDFGGSLPEWGFSESPLLDGNKIAVTPGGAQGAIAALDKKTGAPLWQSKEFTDAAQYA
                     SLVPATIGGVRQYIQLTMESVAGVAANDGKLLWRAPRKGRVAVIPTPIVKDDLVYVTS
                     GYEVGCNLFKIVRDSATFDATRVYANKTMANHHGGAILVGDYVYGHSDRGGWTCQNLA
                     SGETAWQEKAKLDKGSLTYADGRFYCREEKQGSSEVALLEATPVAYVEKGRFKQPDQS
                     GKQAWPHPVIANGKLYLRDQDLLFCYDVKAQ"
     gene            1894..5505
                     /gene="nifJ"
                     /locus_tag="PKK20_02430"
     CDS             1894..5505
                     /gene="nifJ"
                     /locus_tag="PKK20_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNU98777.1"
                     /translation="MKSFTTIDGNEAVAYVAYRCNEVMGIYPITPSSNMGEWCDQWAS
                     EGVKNLWGTIPSVVAMQSEGGAAGTVHGALQTGALTCTFTASQGLLLKIPNMLKIAGE
                     LTPTVFHISARALATHALSIFGDHSDVMLARTTGFAMLSSDSVQEAMDFALIAQAATL
                     ESRVPFLHFFDGFRTSHEVSKIELISDDTMRAMISEEAVIAHRQRAMNPDRPVLRGTA
                     QNPDVFFQAREACNPYYLKCADVTQRAMDKFRQLTGREYKVFQYSGAPDATDIVVLMG
                     SACETADETVDFLNAQGGKYGVVRVRLYRPFDVKRFIECLPPTVKTVSVLDRTKEPGA
                     GGEPLYLDVVAALVEGLPEGYGQLKAMPRVLGGRYGLSSKEFTPAMLKGVFDNARSAQ
                     PKNHFSVGIEDDVTHFSLPYDPDFSTEGEKVVRAMFYGLGSDGTVGANKNSIKIIGES
                     TGNYAQGYFVYDSKKAGAVTVSHLRFGPTPIRATYLVNRANFVACHQPVFLERYDMLQ
                     PLVPGGTFLLNTPFGADEIWDRLPRPMQLQMVNKKAKFFVIDAIKVARASGMGSRINT
                     VMQTCFFALSGVLPRDQAIEQIKYTIKKTYGRKGEDVVRKNIEAVEHTLTHLHEVKVP
                     AQITSGFDIPDPFTADAPSRIKETLGKIYGGQGDSLPVSAFPCDGTFPTATTQYEKRN
                     LALEIPVWDPKTCIQCGKCTVVCPHGAIRMKVYDAAALDGAPATFKSCDARAPEWKGM
                     KYTIQVAPEDCTGCVLCVDACPAKNKTEVKLKAINMRPQAPLRLPERENWGFFLKIPD
                     LDRRKIKVTQLRQQQVMRPLFEFSGACAGCGETPYIKLLSQLFGDRAVIANATGCSSI
                     YGGNLPTTPYCVNHEGRGPTWNNSLFEDCAEFGLGFRVSIDKQREFACELLRRMTPTL
                     GDDLVNAIITADQTMESGIYDQRERVAALKQKLQGNSSSEARMLLSVADNLVRRSVWM
                     LGGDGWAYDIGYGGLDHVLASGRDVNVLVLDTEVYSNTGGQMSKATPRGAVAKFAAGG
                     KPLAKKDMGLIAMTYGTVYVASVAMGAKDEHTLRAFIEAESYPGPSLIIAYSHCIAHG
                     INLDAGIGMRQQKSAVESGQWLLYRFDPRRAAAGENPLQLDSQAAKTRIADFLMSENR
                     FKMLTKSKPEDAKRFFEMAQKDAEARWQLYTYMAARKLGGNGTPAAPAPAPAPAAKA"
     gene            5565..6560
                     /locus_tag="PKK20_02435"
     CDS             5565..6560
                     /locus_tag="PKK20_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNU98778.1"
                     /translation="MDFSTTYLGLKLRSPLVLSACPLTQEVDDIKRAEDSGAGAIVLP
                     SMFEEQLRQERAELDAALEQGTQSFSEALSYFPDVGEFRLGGEDYLEHIAAAKAATKI
                     PVIASLNGASLGGWTDFAKKIQTAGADALELNIYYIPTDPDLTSADVEKTYLDILKAV
                     KQAVTIPVAVKLSPFFSSMANVARELDRAGADGLVLFNRFYQPDIDLETLEVEHSLLL
                     STPQARRLPLRWVAILHGRVKASLAATSGIHTASDALKVLMAGADVAMLCSVLLRKGL
                     PQIAAIEKEMRQWMEQHEYDSIEQLKGSLSQKNCPDPAAFERAQYMRVISSWRPA"
     gene            6866..9304
                     /locus_tag="PKK20_02440"
     CDS             6866..9304
                     /locus_tag="PKK20_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNU98779.1"
                     /translation="MQDYEKLGVFYLGRRYDLGKNQSLPELLLYESKHLVTHGLVVGM
                     TGSGKTGLCFDIVEEAAMDGIPSIVIDPKGDLGNLLLTFPDLRPEDFAPWISEDDARK
                     KGLSMTDYAAQQAELWRQGLAQWNQDGERIRRLRAAADFALYTPGSSAGRPVSILESF
                     AVPPPETLEDGELLRERVGSMVTGLLGLIGVRADPVKSRESILLANLLQSAWSGGRDL
                     DLAALIREVQEPPVRRIGVLDVESFYPAKERFELAMQLNNLLASPSFAAWMQGEALDV
                     GRFLYTDAGKPRVAIFSIAHLGDSERMFFVTLLLNQILSWMRGQSGTNSLRALVYMDE
                     IFGYFPPVADPPSKQPLLTLLKQARAYGVGLVLATQNPVDLDYRGLANIGSWFIGRLQ
                     TDRDKARVLEGLEGAAAGRGVKFDRAAAEQLLAGLASRIFLLHNVHDDEPDIFESRWA
                     MSYLRGPLSRSQIKSLNDAHQDTPSIAPPAAAGPAGPSTPVAPVPVGGRSSRPVLPPQ
                     VPQFFAPVRGVPPPGSTIIYQPRVLGSAQVRYANAKFKVDLLQDLIVATEITDSAVPV
                     DWTLAEELAITANQLEKSPVAAEFASCPPAAGDAKNYAAWAKDFATWIQNQRRLTLLR
                     SPSLGQVSKPGEEERDFRIRLRQAAREARDEAAEKLRAKYAPKMATLQERLRRAEAAR
                     QREIDQAKRAKMDTVISFGSTLLGAFLGRRAVSLGTVSKAGTAMRSAGRAMGQAGDVG
                     RAEETVAAVQQQLENLESQFEAEKMAQTEDLPVETLETIELSPTRSNINVRLVALVWL
                     PFIRAAAGVTNPAY"
     gene            complement(9402..11201)
                     /gene="aspS"
                     /locus_tag="PKK20_02445"
     CDS             complement(9402..11201)
                     /gene="aspS"
                     /locus_tag="PKK20_02445"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNU98780.1"
                     /translation="MKRTHHCNELRPKHIGQTVSLSGWVHSRRDLGGVIFIDVRDREG
                     RTQAVFDPSHLSAKVFERAAGLRSESVIRVGGAVRQRPDGTVNPKISTGEVEVLVQDL
                     EVLNLADPLPFQIDDPESAAKVNEELRLKYRYLDLRRGDMARNLRLRHAVATATRAYF
                     DEHGFLEIETPMLFKSTPEGAREFLVPCRTNPGLFYALPQSPQQFKQILMVAGIERYF
                     QLAKCFRDEDLRADRQPEFTQIDIEMSFIDREDIYATIEGLLSRIWKRALNRDLATPF
                     RRLPFREALDRFGSDKPDLRFGLELEDFTEAFRASEFKVFSDAVKAGGVVKAINAKGL
                     AGVTQGQMEAMTQIAKDSGAKGLAFIKVENGEWKAPMVKFFTDAEKTLLRQKLDIEEG
                     DLILFAAGEWLMACEILGRIRLYAATVLEAQDKLTIPSDRFEFLWVVDFPLLSFDKEQ
                     NRWYSSHHPFTAPVAEDIPFLTTDPRKVRGQHYDVVVNGVELGGGSIRIHQPAVQKKV
                     FEDVLQIPPEIAQARFGYMLEAFRYGAPPHGGIALGFDRLVAMLCGTPSIRDVIAFPK
                     TAKGTCLMTDSPGTADLKQLRDLYLELKIPSEE"
     gene            complement(11520..12362)
                     /locus_tag="PKK20_02450"
     CDS             complement(11520..12362)
                     /locus_tag="PKK20_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU98781.1"
                     /translation="MSLRVIPFRCRAFTLIELLVVIAIIAILAGMLLPALARAKTKAH
                     GIQCINNLRQLKLAWLAYAHDNGDSVTAPGNNRDEPNSWVGGWLDYQGSNSDNTNTLL
                     LLTERYSKIAPYLQSADVFKCPADKSYVTIGGRRHDRVRSMGMSQALGGPGEWLPTAP
                     GMHAGQKEYRTYYKLSEITEPGPSMLYVLLDEHPDSINAGGFANKMVVNPSSAYIIDY
                     PASYHNGAAGISFADGHAEIRKWKDPRTIKPIAYRDGALTLNVASPNNQDMVWLSERT
                     SVRR"
     gene            complement(12605..13179)
                     /locus_tag="PKK20_02455"
                     /pseudo
     CDS             complement(12605..13179)
                     /locus_tag="PKK20_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997394.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(13170..13259)
                     /locus_tag="PKK20_02460"
                     /pseudo
     CDS             complement(13170..13259)
                     /locus_tag="PKK20_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13347..>14390
                     /locus_tag="PKK20_02465"
     CDS             13347..>14390
                     /locus_tag="PKK20_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98782.1"
                     /translation="MKTNRPISDPTSVNRRGFLKTSVTVAGTALVGALDVGRFAHAAE
                     SATLKLGLIGCGGRGTGAVGDAMHGDSGTVLWAAADAFPDKIETSLSTLTPMFPGRVQ
                     VPRERRFAGLNGYRGVIENCDVVLLACASRFHPDYALAAVRARKHVFIEKPAAVDIVG
                     IRKILEADALSKANGTGVLAGVTYRYHLGRREAIQRVHGGEIGEIVAIQCDYLRGPYR
                     LIDRQAQWSEMEYQLRNWYHFTWLSGDDIPQSLVHNLDSALWALGDVMPDHAYGMGGR
                     STHFQASMGTSFDHHHVTYEYADGRCIYGSCRTATGCFGLNKDVFHGTKGRCHFAAFD
                     RPYFTDLAGKVTWR"
BASE COUNT         2664 a         4578 c         4555 g         2593 t
ORIGIN      
        1 aagagatcga ggtcctcgaa ctcgccgccc agcccgtgca cccggacgac gcccacaccg
       61 tctggaacgc ggcggacgtt ctcgatgtgc tggaccggaa ccgaaatatc gtcgcgtggt
      121 tcaacggaca caatcacgcg ggagccttcg cggtgcgcaa cggcgtgcca ttcgtcacga
      181 tcgcgggcat ggtcgagacg cgcgacgcca acgcctacgc cgtcctgaat ctgtgcgcca
      241 agcacttcga gcttgtcggg cacggacgcc aaaccagtcg gacggctccg tttggcccgg
      301 atgtttcatg aagccccgcg gggtctcaca cccggattcg ggcatgaggc ggcgtcactg
      361 cgccttcacg tcatagcaga acagaaggtc ctggtcgcgc aggtagagct tgccgttggc
      421 gatgaccgga tgcggccagg cctgttttcc gctctgatcg ggctgtttga aacggccttt
      481 ctccacatac gccacaggcg tcgcttcgag aagcgccacc tcgctcgacc cctgcttttc
      541 ctcgcggcag tagaaacggc cgtcggcgta ggtcagcgac cccttgtcca gtttcgcctt
      601 ctcctgccag gctgtctcgc ccgaggcgag gttctggcac gtccagcctc ctcgatcgga
      661 gtgcccgtag acgtagtcgc cgacgaggat cgccccgccg tgatgattgg ccatggtctt
      721 gttcgcgtag acccgggttg cgtcaaatgt cgcgctgtcc cttacgatct tgaacagatt
      781 gcaaccaacc tcgtacccgg acgtcacgta gaccaggtcg tccttgacga tgggcgtcgg
      841 gatgacggcg accctgcctt tgcggggggc gcgccaaaga agcttgccgt cgtttgcggc
      901 gacgcccgcc acgctttcca tggtgagttg aatgtactgt cgcacgccgc cgatggtggc
      961 gggcaccagc gaggcgtatt gggcggcatc ggtgaactcc ttgctctgcc agaggggcgc
     1021 cccggtcttc ttgtccaaag ccgcaatcgc gccctgcgcg ccgcccggtg tcaccgcgat
     1081 cttgttccca tcgagcagcg gggactcgct gaacccccat tcgggcaggc tgccgccgaa
     1141 gtccgtgaca agatgcttgc gccacagttc cttgccggtg gccgtctcga agcaagccaa
     1201 ctcgccatat tggcccaccg caaagagacg gtctccatcc accgttggtg tgcatcgggg
     1261 ccccgcaaaa ccgccccacc ccggcgcgcc ggccttgccc agcctgctcg tccagagctt
     1321 cgtgccgtcg ctctccttga gcgcgatgat gtaattccca tccgccatgt cgcccgcggt
     1381 gaagattctt ccctgggcga ctgagaccgt ggtgaagccg ctgcccaggc cggccgtttt
     1441 ccatgataac cgcggcccgt ccgccggcca tgtcttgagc agaccggtct cggcggagcg
     1501 cccggtgcgg tccgaaccaa accactgcgg ccaatcgacg gcgtggattc ccggggcgag
     1561 caggagctcc agaagcagca acagtgtcgt ctgagcgcca ttcgatagcc gattggttcg
     1621 tttcatggcg cgtcaggtat atgtgttctg gaggtgaagt aaagcctgtt cgcgccggat
     1681 cagcgcaaaa tatgcgaagc agtcggcaaa gcatgtggag ccgatcttgg ggcgggccgg
     1741 gaaggtgatg tgtattgaca gcgacaacgc ggagcgctcc gcggcgtccg agccgcggac
     1801 tggcggctgc ggacggcgtt cgtccgcgcc tcgtcccgat cagggacggc atgggagcgc
     1861 gcgggggtca acaacctgga attggaatcg atcatgaaga gcttcacgac aattgatggc
     1921 aacgaggcag tcgcctacgt cgcttaccgc tgcaacgagg tgatgggcat ttacccgatc
     1981 acgccctcct cgaacatggg ggaatggtgc gatcagtggg cctccgaagg ggtcaagaat
     2041 ctctggggca ccattccgag cgtggtggcg atgcagagcg agggcggcgc cgcgggcacg
     2101 gtgcacgggg cgttgcagac cggggcattg acctgcacgt tcacggcgtc gcaggggctt
     2161 ctgctgaaga ttcccaacat gctcaagatc gccggggaac tgacgcccac ggtgttccat
     2221 atcagcgcgc gcgctctggc cacgcacgca ctctcgatct ttggggatca cagcgacgtc
     2281 atgctggccc gcaccaccgg gtttgccatg ctcagttccg actcggtaca ggaggcgatg
     2341 gacttcgcgc tgattgcgca ggcggcgacg ctcgaatcgc gcgtgccgtt cctgcacttc
     2401 ttcgacggct tccgcacgtc gcatgaggtg tccaaaatcg agctgatttc cgatgacacc
     2461 atgcgggcga tgatcagcga agaagcggtc atcgcgcacc gccagcgcgc aatgaacccc
     2521 gatcgccccg tcctgcgcgg cacggcccag aacccggacg tcttcttcca ggcgcgcgag
     2581 gcgtgcaatc cgtactattt gaagtgtgcg gacgtgaccc agcgggcgat ggacaagttc
     2641 cgccagttga ccggacgcga gtacaaggtg ttccagtact cgggcgcgcc ggacgccacc
     2701 gacatcgtcg tcctgatggg ttcggcctgt gagaccgcgg acgagacggt tgactttctc
     2761 aacgctcagg gcgggaagta tggcgtggtg cgcgtgcggc tctatcgccc gttcgacgtg
     2821 aaacggttca tcgagtgcct tccgccgaca gtcaagaccg tttcggttct cgatcggacc
     2881 aaggagcccg gggcgggggg tgaaccgctt tatctcgatg tggtggcggc tttggtggag
     2941 gggcttccgg agggttacgg gcagctcaag gcgatgccgc gcgtgctggg cgggcgctac
     3001 ggcctatcat cgaaggagtt cacgccggcg atgctcaagg gcgtgtttga caatgcccgc
     3061 agcgcgcagc cgaagaacca cttcagcgtc ggcatcgagg atgatgtcac gcatttcagt
     3121 ctcccctacg atccggactt ctcgaccgaa ggcgagaagg tggtgcgggc gatgttctac
     3181 ggcctgggat ccgacggcac ggtgggcgcg aacaagaact cgatcaaaat catcggcgaa
     3241 agcaccggca actacgcgca ggggtatttc gtctacgatt cgaagaaggc cggcgccgtc
     3301 acggtctcgc acttgcgttt cggtccgacg cccatccggg cgacttacct ggtcaacagg
     3361 gcgaacttcg tcgcctgcca tcagccggtg tttctcgaac gctacgacat gctgcagccc
     3421 ctggtgcccg gcggcacgtt cctgctcaac acgccgttcg gcgccgacga gatctgggat
     3481 cgccttccgc gcccgatgca gctccaaatg gtgaacaaga aagcgaagtt cttcgtgatc
     3541 gatgccatta aagtggcccg ggcttcgggc atgggcagcc gcatcaacac ggtgatgcag
     3601 acctgtttct tcgctctctc gggcgtgctg ccgcgcgacc aggccatcga gcagatcaag
     3661 tacaccatca agaaaaccta cggcaggaag ggcgaagacg tcgtgcgcaa gaacatcgag
     3721 gccgtcgagc acaccctcac ccatctgcat gaggtgaaag tcccggcgca gatcaccagc
     3781 ggattcgaca ttccggatcc gttcaccgcc gacgcgccca gccgcatcaa ggagaccctt
     3841 ggcaaaatct acggcgggca gggcgattcc ctcccggtga gcgccttccc ttgcgacggc
     3901 acgttcccca ccgccacgac ccagtacgag aagcgcaacc tcgccctcga gatcccggtc
     3961 tgggacccca agacctgcat ccagtgcggc aaatgcactg tcgtctgtcc gcacggcgcc
     4021 attcggatga aggtgtacga cgctgcggct ctcgacggcg cgcccgccac attcaagtcg
     4081 tgcgacgccc gggcgccgga gtggaagggc atgaagtaca ccattcaggt cgcgcccgag
     4141 gactgcaccg gctgcgttct ctgcgtggac gcgtgcccgg cgaagaacaa gaccgaggtg
     4201 aagctcaagg cgatcaacat gcggccccag gccccgctgc ggctccccga acgcgagaac
     4261 tgggggttct tcctaaaaat ccccgatttg gaccgccgga agatcaaggt gacgcagttg
     4321 cggcagcaac aggtgatgcg tccgctcttc gagttctccg gcgcgtgcgc gggctgcggc
     4381 gagacgccgt acatcaagct gctctcccag ctcttcggcg atcgcgccgt gatcgccaac
     4441 gcgacgggct gcagttcgat ttacggcggc aacctgccta ccacgcctta ctgcgtgaat
     4501 cacgagggac gcgggccgac ctggaacaac tccctgttcg aggattgcgc cgagttcggt
     4561 ctcgggttcc gggtgtcgat cgacaaacag cgcgaattcg cgtgcgaact cctgcggcgc
     4621 atgacaccca ccctcgggga tgacctcgtc aacgccatca tcaccgcgga tcagaccatg
     4681 gaatcgggca tctacgacca gcgcgagcgg gtcgccgccc tcaagcagaa gctccaaggg
     4741 aattcctcga gcgaagcccg gatgctcctg tcggtcgccg ataatctggt ccgccgcagc
     4801 gtgtggatgc tgggcggcga cggctgggcg tacgatatcg ggtacggcgg gctcgaccac
     4861 gtgctggcca gcgggcgcga tgtgaacgtg ctggtgcttg acaccgaggt ctactcgaac
     4921 acgggcggtc agatgtcgaa agccactccg cgcggcgccg tggccaagtt cgccgcgggc
     4981 ggaaagcccc tggccaaaaa ggacatgggc ctcatcgcga tgacttacgg cacagtgtat
     5041 gtcgccagcg tcgccatggg cgccaaggac gagcacacgt tgcgggcgtt catcgaggcc
     5101 gagtcctatc ctggcccctc cctgatcatc gcctacagtc actgcatcgc ccacggcatc
     5161 aacctggacg caggcatcgg gatgcggcag cagaagagcg ccgtcgagtc cggtcagtgg
     5221 ctgctctatc gcttcgaccc gcgccgcgcc gcggcgggcg agaacccgct ccagctcgat
     5281 tcccaggctg ccaagacgcg tatcgcggac ttcctgatgt ccgagaatcg cttcaagatg
     5341 ttgaccaaga gcaagccgga agatgccaag cgattcttcg aaatggccca gaaggacgcc
     5401 gaagcgcgct ggcagttgta cacctacatg gcggcgcgca agctgggagg caacggcacc
     5461 cctgcggcac cggcaccggc gccggctccc gcggcgaagg cctgaggaac gcaatgactg
     5521 ccattggcga gaactgaata cttggaacga gaaaggaacc aattatggac ttcagcacca
     5581 cttatctcgg attgaaactg cgcagtcctc tcgtgctctc cgcctgcccg ctgacacagg
     5641 aggtggacga tatcaagcgc gcagaggatt ccggggccgg cgccatcgtg ctcccctcga
     5701 tgttcgaaga gcagctccga caggaacgtg cggagctgga tgcagcgctc gaacagggga
     5761 cccagagttt ctcggaggct ctgagctatt tccccgacgt cggcgagttc cgcctgggcg
     5821 gcgaggatta cctcgagcac attgcggcgg ccaaggccgc aaccaagatt cctgtcatcg
     5881 ccagcctgaa cggggcgtcc ctgggcggtt ggacggactt cgccaagaaa atccagacgg
     5941 ctggggccga cgccctcgaa ctgaatatct actacatccc caccgacccc gatttgacat
     6001 ccgccgatgt cgagaagacg tatctggata tcctcaaagc ggtcaagcag gccgtcacga
     6061 ttcccgtcgc ggtcaagctc agccccttct tcagcagcat ggccaacgtc gcgcgcgaac
     6121 tcgatcgcgc gggcgccgac ggcctggtcc tgttcaatcg gttttatcaa cccgacatcg
     6181 atctcgagac gctcgaggtc gagcacagtc tcctgctgag cacgccgcag gcccgccgcc
     6241 tgccactgcg ctgggtcgcc atcctgcacg gccgcgtcaa ggccagcctg gccgcgacca
     6301 gcgggattca caccgcttcc gatgccttga aggtgctcat ggccggcgcg gatgtggcga
     6361 tgctctgctc ggtgctgctc cgcaaaggct tgccccaaat cgccgccatc gagaaggaga
     6421 tgcgccagtg gatggaacag cacgagtacg attcgatcga gcaactcaag ggttcgctga
     6481 gccagaagaa ctgccccgac ccggccgcct tcgagcgcgc gcagtacatg cgggtaatct
     6541 cgagctggcg cccggcgtga cgcggggtgc tcctcgagcc cgcattggtc ggcgtgttgg
     6601 agttctcagc gtcgcgctga tctccgctcc atgaaacatg cgggcaggga cagcaagcgc
     6661 ggcccgtgag ctgatcccga ctcaaacgaa atgcctgccg ggcagcctat gccgggcagg
     6721 caggtggcag gcaggcggca ccgttccact tcatgcatcg atcctgtttc gtcttgaaca
     6781 actgcctgcg cgcgagcagg gttcagcggt gtgtaaaagc gacctttcat gatttgcgac
     6841 gtccggtgtc gaactgactt tcactgtgca agactacgaa aagctcggcg ttttctatct
     6901 ggggcgtcgc tacgacctgg gcaagaacca gagccttccg gagctcctgc tctacgagtc
     6961 caagcacctc gttacccacg gcttggtcgt gggcatgacc ggcagcggca agaccggatt
     7021 gtgcttcgac atcgtcgagg aagcggccat ggacggcatc ccgtccatcg tcattgatcc
     7081 caaaggcgat ctgggcaatc tgctcctgac attccccgat ttgcgtccgg aggactttgc
     7141 gccctggata agcgaggacg acgcgcgcaa gaaagggctg tcgatgaccg actacgcggc
     7201 gcagcaagcc gagctttggc gccaaggcct cgcccagtgg aaccaggatg gcgagcgcat
     7261 tcggcgattg cgggcggcgg ccgatttcgc cctctacacg ccggggagca gcgccggtcg
     7321 cccggtctca atcctcgagt cgttcgcggt ccctccgcca gaaacgctcg aggacgggga
     7381 attgctgcgg gagcgggtcg gctcgatggt gacaggactg ctcgggctca taggcgtcag
     7441 agccgatccg gtcaagagcc gggagagtat cctgctcgcc aatctcttgc agagcgcctg
     7501 gagcggaggc cgcgacctcg atctcgccgc cctgattcgc gaggtccagg agccacccgt
     7561 gcgccgaatc ggcgtgctcg atgtcgaatc gttctaccct gccaaggagc ggttcgaact
     7621 ggccatgcag ctcaacaacc tgctcgcctc gcctagcttc gccgcatgga tgcagggcga
     7681 agctcttgac gtcggacgat tcctttacac cgatgccggc aaaccgcgcg tggccatttt
     7741 ctcgattgcg catctgggcg attcggagcg gatgttcttt gtcactctgc tgctcaacca
     7801 aatcctgagc tggatgcgcg gtcagtccgg caccaacagt ctgcgcgcgc tcgtgtacat
     7861 ggacgagatt ttcggctact tccccccggt tgccgatccg ccctcgaagc agcctttgct
     7921 gactctgctc aaacaggcgc gcgcctatgg ggtgggtctg gtcctcgcca cgcagaaccc
     7981 agtcgatctc gactaccgtg gcctggccaa catcggttcc tggttcatcg ggcgactcca
     8041 gaccgaccgg gacaaggccc gcgttctcga aggcctcgaa ggcgccgccg ccgggcgggg
     8101 cgtgaagttc gatcgcgccg ccgccgagca actgctggcc ggattggcca gccgcatctt
     8161 ccttctccac aacgtccacg acgacgagcc ggatatcttc gagtcgcgtt gggcaatgtc
     8221 ctatctgcgc ggcccgcttt ctcggtcgca aatcaaatcg ctcaacgacg cgcatcagga
     8281 cacgccctcg atcgctcctc cggccgcggc cggccccgcc ggcccatcca ccccagtcgc
     8341 ccctgtaccg gtcggcgggc gatcgagccg ccccgtgctg ccgccgcaag tgccgcagtt
     8401 cttcgcgccg gtgcggggtg tgccgccgcc gggcagcacg atcatctacc agccgcgcgt
     8461 gctcggcagc gcgcaggtcc ggtacgccaa cgcgaagttc aaggtggacc tgctgcagga
     8521 tctcatcgtg gcgaccgaga tcacggacag cgccgtgccc gtggattgga ccctggcgga
     8581 ggaactcgcc atcacggcaa atcagttgga gaagagcccg gtggcggctg agttcgcctc
     8641 atgcccgccc gccgccggcg atgcgaagaa ctacgcggcc tgggcgaagg actttgcgac
     8701 ctggatccag aaccagcgca ggctgaccct gctccgatcg ccatctctgg gccaggtctc
     8761 gaagccggga gaggaagagc gcgattttcg aatccgcctg cggcaggccg ctcgggaggc
     8821 gcgcgatgag gccgcggaga agctgcgcgc aaagtacgct ccaaagatgg ccacactcca
     8881 ggagcgcctg cgcagggccg aggcggccag gcaacgcgag atcgatcagg ccaaacgggc
     8941 caagatggat acggtcattt ccttcggctc gacattgctc ggggccttcc tgggccggcg
     9001 tgccgtctct ctcggcacgg tgagcaaggc cggcaccgcg atgcgcagcg cgggccgcgc
     9061 gatgggccag gccggcgatg tcgggcgcgc cgaggaaacc gtggcggctg tgcagcagca
     9121 gttggagaat ctcgagtcgc agttcgaggc tgagaagatg gcccaaaccg aggacctccc
     9181 ggtcgagacg ctcgagacga tcgaactgag ccccacccgc tcgaacatca acgtgcggct
     9241 cgtggcgctg gtctggctcc cgttcattcg cgccgccgcc ggcgtgacca accccgcgta
     9301 ctgagtgccc gctcgacgga cgctccaggc cgccaccagt tggggcctct cccgcggtcg
     9361 cgggatcggc cctccgtcgt tcttcgccgg ggcgaggcgt gttactcttc cgatgggatc
     9421 ttcagttcca ggtacaggtc ccgcagttgc ttgaggtcgg ctgttccggg agaatcggtc
     9481 atgaggcagg ttcctttcgc cgtcttcggg aaggcgatga catcgcggat gctcggcgtg
     9541 ccgcacagca tggcgacgag acggtcgaat ccgagcgcga tgcctccgtg cggcggcgcg
     9601 ccgtatcgga acgcttcgag catgtatccg aagcgcgcct gggcgatctc gggcggaatt
     9661 tggagcacgt cttcgaacac ctttttctgg acggcgggtt ggtgaatacg gatggacccg
     9721 ccgcccaact cgaccccgtt gaccaccacg tcgtagtgct ggccgcggac ctttctgggg
     9781 tcggtggtca ggaatggaat gtcctccgcc acgggcgccg tgaagggatg atggctcgaa
     9841 taccagcggt tctgctcctt gtcgaaactg agcagcggga agtcgacaac ccagaggaac
     9901 tcgaagcgat cggatgggat cgtcagtttg tcttgcgctt cgagcacggt cgcggcgtag
     9961 aggcggatgc gaccgaggat ttcgcacgcc atcagccatt cgcccgccgc aaacaggatc
    10021 aggtcgcctt cctcgatatc gagtttctgc cgcagcaagg tcttctcggc gtcggtgaag
    10081 aatttgacca tcggcgcctt ccattcgccg ttctcgacct ttatgaaggc gagtcctttg
    10141 gcgccgctgt ccttggcgat ttgcgtcatg gcctccatct ggccctgggt caccccggcg
    10201 agtcccttgg cgttgatggc cttgaccacg ccgccggcct tgacggcgtc cgaaaacacc
    10261 ttgaactcgc tggcgcgaaa cgcctcggtg aaatcctcga gctcgagccc gaatcgcaaa
    10321 tccggtttgt cgctgccgaa gcggtcgagc gcctcgcgga acggcagacg ccgaaacggg
    10381 gtggccagat cgcgattcag agccctcttc cagatgcgac tcaacaggcc ctcgattgtg
    10441 gcgtaaatgt cctcccgatc gatgaacgac atctcgatgt cgatctgcgt gaactccggc
    10501 tgccgatccg cccgcaaatc ctcgtcccga aaacacttgg caagttggaa ataccgctcg
    10561 atgcccgcga ccatcagaat ctgtttgaac tgctgcgggg attgcggaag ggcataaaac
    10621 agcccgggat tggtccggca cggcaccagg aactcgcgcg ccccctcggg cgtcgacttg
    10681 aacagcatcg gcgtctcgat ctcgagaaag ccgtgctcgt cgaaataggc gcgcgtggcg
    10741 gtcgccaccg cgtgccgcaa acgcaggttg cgcgccatgt cgccgcggcg cagatccaga
    10801 tagcggtact tcaaccgcag ctcctcgttg accttcgcgg ccgactcggg atcgtcgatc
    10861 tggaagggca ggggatcggc caggttcaag acctcgagat cctggaccag aacctcgact
    10921 tcgcccgtgg aaatcttcgg attcaccgtg ccgtccgggc gttggcgcac cgctccgcca
    10981 acgcggatca cgctctcact ccgcagcccg gccgcccgct cgaagacctt cgccgacaga
    11041 tgcgaaggat caaacaccgc ctgcgtgcgg ccctcgcggt cgcgcacgtc gatgaagatg
    11101 acaccgccga gatcgcgacg ggaatggacc cagcccgaga gagatacggt ttgcccgatg
    11161 tgtttcgggc gcaattcgtt gcagtgatgt gtacgtttca tgaacaacag cgcgactcac
    11221 cagcccgcat ctctccgacc ctgaacacca tggctggaca ccgggcttcg aagcccaaga
    11281 ggcgggcacc ccagcggcgc gtgacctcac gcaaccggct gtgcctgcac caagcccggt
    11341 gtggaacatc ggatcgagcg aaccgcaaac gggggcggat ggtggggccc ggacctggaa
    11401 tttcaaagtg aattctttcc gcttcctgcg tcgcggaagg aagcagcgcc ttgtggcgag
    11461 gcatcgaagg ctcgggtgtt agaactcgcg aagctctatg gaacatccgg gcgaaggctt
    11521 caccgcctca ccgatgtgcg ttcggacagc cacaccatgt cctggttgtt gggcgaagcc
    11581 acgttgagag tcaacgcgcc atcgcgatag gcgatcggct tgatcgtgcg ggggtccttc
    11641 cactttcgaa tctcggcatg gccatcggcg aatgaaatgc cggccgcccc attgtggtag
    11701 ctggcggggt agtcgatgat gtaggcgctc gagggattga ccaccatctt gttggcgaac
    11761 ccgcctgcgt tgatgctgtc gggatgctcg tcgagcagga cgtagagcat cgaagggccg
    11821 ggttcggtga tttcgctcag cttgtagtac gtccggtatt ccttctgtcc cgcgtgcatg
    11881 cccggcgctg tgggcagcca ttcgcccggc ccgcccaacg cctggctcat ccccatgctg
    11941 cgcacgcggt catgccgcct cccgccaatg gtgacgtagc ttttgtcggc agggcacttg
    12001 aagacgtccg cactctggag ataaggcgcg atcttgctgt aacgctcggt cagcaggaga
    12061 agcgtgttgg tgttgtcgct gttgctcccc tgataatcga gccagccgcc gacccacgag
    12121 ttcggctcgt cgcggttgtt gccgggcgcc gtgacgctgt cgccgttgtc gtgcgcgtac
    12181 gccagccacg caagcttcag ttgcctcaga ttattgatgc actggatgcc atgggccttg
    12241 gtcttggccc tggccagcgc cggcagcaac atgccggcga gaatggcaat gatggcaatg
    12301 accacgagca actcgatcag cgtaaacgcc cggcatcgga agggaatcac gcgcagcgac
    12361 atactcacaa tcttcggttt gttggtgacg ccccgactgt agcatcgctt tcgccaaagg
    12421 cgagcccgga aattgtccgt tgcctaccac cccaacctgg ggctggagcg tcagaccacg
    12481 cgcagccttg gcgaaggcaa tggggtcgca tctaaatagt tgacaatcga gaattgactc
    12541 cctttccggg cgcggttttg cccatcagcc cggggcatgt cgagcgcgca ggagtcggta
    12601 tgggctagtc cggcgcccgc cgatagcgct tggctgcaat cgcgagggcg acaacgccga
    12661 agagcgcaat cggccagggt cgctgcatca cctcggccac ttcgatgccc ttgagcatga
    12721 tgcctcgaac gagccggaga aaatgcgtga gggggaggat ttcgcccagg tacagcgccc
    12781 aggcgggcat gccgcgaaac gggaacatga accccgagag caggatggaa gacaggaaca
    12841 cgaagaaggc catctgcatc ggctgaagct ggttgcgccc aatgatcgag aaggtgatgc
    12901 ccatcgccgg attggccaca atgaacacga acgcggcgac cagcaacatg gtgacgctgc
    12961 cgactatagg cacgccaaag aggaagtaac ccgccgccag aatcgccgcg acctggatgt
    13021 agccaatcgg tatgtaggga atgatcttgc cgatgagaac ctccaacgga cgcgtgggca
    13081 tcgcgagcag gttctccacc gtgccccgct cccgttcgcc cgtgatggcc agacccctga
    13141 tcaggatcat catcggtgca tctccccccc cagaagtccc gccgcgcctt cgggtccacg
    13201 cccgcggtcg gttcgtccag caacagcaac ttcggctcgt ggatcatgca ggcagccaga
    13261 tcggaaaagc cagatcggaa aggctggcac gatccgatcg cagtggtact ctgggcgcgc
    13321 cactgattca ccgaaagaac acgcacatga aaacgaaccg tccaatctcc gatccgacat
    13381 ccgtaaaccg ccgcggcttt ctcaagacct cggtcacagt cgctgggaca gcacttgtcg
    13441 gcgcactcga tgtggggcgc ttcgcgcacg cggccgagag cgccacactc aagctcggcc
    13501 tgattggctg cggcggacgc ggcacgggcg cggtcgggga cgcgatgcat ggcgactcgg
    13561 gcaccgtgct ctgggcggct gccgatgctt ttcccgacaa gatcgagact tcgctcagca
    13621 cgctgactcc gatgtttccg ggtcgggtcc aggtgccgcg ggagaggcgg tttgcggggc
    13681 tgaacgggta tcgcggcgtg atcgagaatt gcgatgtagt cctgctcgcc tgcgcgtccc
    13741 ggtttcatcc ggactatgcc ctggcggcgg tccgggccag gaagcacgtg ttcatcgaga
    13801 agccggcggc ggtggatatt gtcgggatcc ggaagattct cgaagccgac gcgttgtcca
    13861 aagcgaacgg gacaggggtg cttgcgggcg tgacgtatcg ctatcacctg gggcggcgcg
    13921 aggcgataca gcgggtgcac gggggcgaga tcggcgagat tgtggcgatt cagtgtgatt
    13981 acctgcgcgg gccctaccga ttgatcgacc gacaggccca atggagcgag atggaatatc
    14041 agttgcgcaa ttggtatcac ttcacctggc tttcgggcga tgacatccct caatcgctcg
    14101 tccataatct cgactcggcc ctgtgggcgt tgggcgatgt gatgcccgac cacgcctatg
    14161 ggatgggagg gcgctcgacg catttccagg cctcgatggg cacgtcgttc gaccatcatc
    14221 acgtcaccta tgaatacgcc gatggacgct gcatctacgg atcctgccgg acggcgaccg
    14281 gctgtttcgg attgaacaaa gacgtgtttc acggcaccaa gggccgctgt catttcgccg
    14341 ctttcgaccg tccgtatttc accgatctgg cgggtaaggt cacctggcgg
//
LOCUS       DAOQRO010000035        14318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3291_length_14318_cov_4.668443, whole genome shotgun sequence.
ACCESSION   DAOQRO010000035 DAOQRO010000000
VERSION     DAOQRO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_14318_cov_4.668443"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKK20_02470"
     CDS             <1..195
                     /locus_tag="PKK20_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98783.1"
                     /translation="GADPKRTGPQGKTILAVAREQGHTSVVEYLEARLAQKTEPAGGQ
                     PRGRALIDGGVGVSEVLDGF"
     gene            227..1993
                     /locus_tag="PKK20_02475"
     CDS             227..1993
                     /locus_tag="PKK20_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNU98784.1"
                     /translation="MRLTAFFATVVLIPFAAHCVWGAEPGNDHRLDEFFGAYLDRWFA
                     LRPLDATRLGDHRFDHRLDDLSPGARAQWIALMRETLEALPEQVDYRALTRSGQIDFE
                     ILRHELVKSLWLAENARPYEEDPRIYNDYISDSVFLLLTQSSLPKETNITNALARMEQ
                     IPQVIAAARANLRNPPAAHIETAIGQNRGAIAFYERGIFELAVSTPQRAALEAAAARV
                     AESLRGYQRFLESDLRARAPGEWRLGRERFCRKLELEVDGGVTADEVLADAQREFERV
                     ERDMFTVARQLWSRFHPGAPLPPDDSAGRRAAVRSVLAAIAQDHSTAETVVADVRRVM
                     ADVKRFIADARIVRLPQPDQCEIIEMPEFQRGNSTAYMNSPPPLDPRAGGYYAVSPPP
                     RDWDADRVRSYFEEYNSQMLRILTIHEAYPGHYVQLEYANRNPSLVRRVLQSGVYIEG
                     WAVYTEAMMLDQGFGEGSLPLRLTQLKFYLRAVANAILDHKMHCADMSDDEALRFLTE
                     DAFQSEGEARLKIVRAKQSSVQLSTYFAGRMAMGRLRQAIQREMGERFALERYHEAVL
                     EPGAVPVKYLPELVRARLGQPR"
     gene            2265..3470
                     /locus_tag="PKK20_02480"
     CDS             2265..3470
                     /locus_tag="PKK20_02480"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNU98785.1"
                     /translation="MTRTLNLDPIDALIADIARGRMVIVVDDADRENEGDLIMAAEHV
                     TPMAVNFMARFARGLICVPTTSERIEQLGIKQMVTENRESFKTDFQVSVDAARGITTG
                     ISAADRCRTIQVMADPTATPTDLTQPGHVFPLRCKPGGVLQRAGHTEAAVDLALLAGC
                     RGIAAICEIMNTDGSMARLPELRKFARRHRLRLGSIQALIQYRRCREKLVERIETVLL
                     PTEHGDFDLHLYRSRLDGQHHVALVCGEVAGQKNVLVRVHSECLTGDVFGSRRCDCGV
                     QLHQAMRQVAEAGRGVIVYMRQEGRGIGLGPKIQAYKLQERGYDTVEANHKLGYAADL
                     REYGLGAQILVDLGLKTIRLLTNNPKKLVGLEGYGLEIVEQLPIRVAPNPHNVKYLRT
                     KREKMGHTL"
     gene            3685..4185
                     /gene="ribH"
                     /locus_tag="PKK20_02485"
     CDS             3685..4185
                     /gene="ribH"
                     /locus_tag="PKK20_02485"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNU98786.1"
                     /translation="MLRKIKTSQARACGGRFAIVASRYNARYVDAMVRAARAELRRAG
                     VGHVDVVRVPGAFEIPVVAARLSRGVGIRPDAVICLGVVLRGETSHAEHIGRGVTAAL
                     IQLQIQTGVPMIHEVLVLEDEGQARVRCLSRDHNRGTEAAQSAVEMVRLVRHLPALEE
                     DDDVPF"
     gene            4467..5717
                     /locus_tag="PKK20_02490"
     CDS             4467..5717
                     /locus_tag="PKK20_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU98787.1"
                     /translation="MQAAPARSGGFTRGQWMVLIAAFLGWMFDGVEMGIFPVVARPAL
                     QEILGTEADQAVGPWMGYITALFLVGAAGGGLVFGWLGDKVGRVRAMTASILAYSLFT
                     GACYFAQAAWQLGLLRFLAALGMGGEWSLGVALVMECWPEAKRPLMAGIIGAAANVGY
                     ALVGVVTWLIPVRPDSWRWVMLAGAIPALLAFFLVLVVPESQRWKASVQRARAEPLRI
                     VFGAGLWRTTLLAIAFGAIALIGTWGSVQWLPLWGDQLVGKSFPQVKGVIMLALSAGA
                     ILGCFIGPWIGAVLGRRMAYFTLCACSLLSCAVLFRIVDSFGPAFVVGSFVVGAATAA
                     FYGWLPLYLPELFPTRARATGQGISFNAGRILAAGGALTQGQLVSHYGGSYAQAGAVV
                     TLIYVVGMVLIWFGPETKGKPLPE"
     gene            5811..6224
                     /locus_tag="PKK20_02495"
     CDS             5811..6224
                     /locus_tag="PKK20_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98788.1"
                     /translation="MLVKMVAILAGCLSMGLAFGQESSTNKPPERVAASVQAPGSIPG
                     SRVVTSISRPAPAAPRAGVTIDGAIPKAIRSKSPLRMISPFAPKEYGDGTDVLAVHPM
                     TGEAQGVTLLRLTYPDRPKPPKSRKSGPFRSPSGT"
     gene            6250..6486
                     /locus_tag="PKK20_02500"
     CDS             6250..6486
                     /locus_tag="PKK20_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNU98789.1"
                     /translation="MELVTVFSTFSPAEAQLVRSRLEAAGIPANVIHETASLSIEGYS
                     VAAGGIEVQVAGDRSEEAKALLADAQEPPPAAGA"
     gene            6483..7892
                     /locus_tag="PKK20_02505"
     CDS             6483..7892
                     /locus_tag="PKK20_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNU98790.1"
                     /translation="MKAALRPLARALPSGELTADPAICQAHAGDKWLASHVPDAVALP
                     RSTESVVSILSFAHRRGIPITARGAGHGYVGGCVPVQGGIVLSLARLNRIREIHPRDF
                     VAVVQSGVITQKLQDTVERRGLFYPPDPASRSECSVGGNIATNAGGPRCLKYGVTRDY
                     VLGLEVVLADGIVVRLGSRTHKNKTGFDLARFFVGSEGLLGVVTEATLKLLPLPPYRA
                     ALAAGFGTTRDASRALQSVFRAGFLPCALEVADAFTLAAAAKRTGSRRLAGCEAHLIA
                     ELDGQSRSVRSELREVERVIGQHRPRFIERALGAIECERIWQLRREFSYALRDTGLTK
                     LNEDIAVPRSRLEALFAFAARLQRKHGLPVACFGHAGDGNIHTNIMVDRTQPGAETRS
                     RAALDDLFRQVLAWGGVITGEHGVGLAKMPWWNQAVSPEVRQLHAAIKRALDPKGILN
                     PGKFVSLGAGDPSHRQRAW"
     gene            complement(8055..8696)
                     /locus_tag="PKK20_02510"
     CDS             complement(8055..8696)
                     /locus_tag="PKK20_02510"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNU98791.1"
                     /translation="MTTLVIATRNHHKAREIRAVLGDGLRYLTLNDFPGAPTTVEDAD
                     TFAGNAAKKADSLARWLAEQPIAREGGALCVLADDSGLEVDALDGAPGVVSARFAALD
                     SAPSANATDIDNTAKLLRLLHGVSTPNRTARFRCVIALATVGQTRPPRLFEGVCEGLI
                     LDAARGANGFGYDPVFQPLGYTESFAELGEPVKNRISHRARALAQAARGLEAP"
     gene            9011..11827
                     /locus_tag="PKK20_02515"
     CDS             9011..11827
                     /locus_tag="PKK20_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNU98792.1"
                     /translation="MLASLAWIVAFAWAGLFRHGLAGAPEVQPQTLIEWTFDREGDQA
                     GWHANDHFADVRVVGGALECRGAGTDPILELGLPLEVAVSHRQALEVRLKASRDGTAE
                     FFWSNTTRGRYGGFSQEKTTRFPVVGDNAWATYRVLPGWQAEGRIIRLRLDLYDNAEF
                     AIDAIRIVSWPELQPVDTREFEFPAADRDWPWIRMGRILSSASPLAGGDRLRLGPTLS
                     IDAEALDYVSMCMTVPSQPEAGRREASPGTPLAAPRLTLWFAGKQDYGFKSVAVPVHA
                     DGQKHVYDVDMLVANGWRGQIVALGIEHTLDVAGQPELHWLKIADRPQGPARLSVVSL
                     GLEPALPRVGKPLRLEAVLANHGGAAARAVTGDLILPDGLAVRGGDVAGRSTPQLGPG
                     ETATWEWALVGDRPVQGQIELRLRADQAEPAQARMAVALTEPVDIGPCDYVPEPRPVR
                     GPYEVGVYYFPGWKSAGQWHPIRPYPERRPVLGWYREGDPEVADWHIKWAVEHGITFF
                     AYDWYWSQGARQLEHALHDGYFKARYRRYLKFCLLWANHNAPGTSSHADCVAVTRHWI
                     EHYFRQPEHLTVEGKPLVIIFSPDRLSADLGSTGVKSAFDAMRATCREAGFPGLLLVA
                     CVGDAAQARQAAAEGYDAVTAYNWPGLDMSAGQMEAPYAALLPAYRRNWQHIIDSAGI
                     PLLIPLCGGWDSRPWHGANNLVRYGRTPELFRQHAQDAAQLSRRTAHLSPLSLLLLVE
                     AWNEFGEGSYIEPHQEFGFGYLDALRDVFTAAPRDHVDVTPPDVGRGPYDVAVHTMPE
                     PVWEFRESDEGWSQTMDLTDVRIENGALIARSTGNDPALFGPPIQARADAYRTAVVRM
                     KLTPRDGRSLPDRAQLFWRTDRLPEGEASSERFDVLADGAWHEYRVDLSGNRRWRGII
                     TRLRLDPCNQRDTQIALDVLRLEP"
     gene            11986..>14318
                     /locus_tag="PKK20_02520"
     CDS             11986..>14318
                     /locus_tag="PKK20_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU98793.1"
                     /translation="MPDAESHRARVVVDGKFFRLGRDKFFARGVTYGPFAPNAAGEHF
                     CSPTQTAHDFDQMRAWGINLLRVYDVPPGWFLELAALKGLWLLVDVPWNKQACLDDAP
                     AREAARRAVREAALACAGHRAVFGISVVNELPPDSVRWSGPASIGSFLEELVGVVKSV
                     DAQCLCTFGSFPPTEYLRVQAIDFVCFNVYLHHPQAFRNYLARLQVLADSKPLVLGEF
                     GLDSLREGEPRQAELLESSIESVFQAGAAGAVVFSFTDAWFKDGLAIEDWHFGLTSAA
                     RVPKPAAFAVQRAYGGAPHFRTVSGPMVSVVVASYNGARTLSACLESLGALSYPVCEV
                     ILVDDGSTDETPEIAARFPRVRYLRHPQNLGLSVARNTGIEAAQGEIVAFTDADCRVD
                     EDWLHYLVSDLVGSRFVGMGGPNLLPSEDSWVAAAVQVSPGGPAHVLLTDQVAEHIPG
                     CNMVFWKWALEEIGCFDPVFRKAGDDVDICWRLQQRGYEIGFSPAAVVWHYRRSTVRA
                     YLRQQHGYGEAEALLERKHPEHFSPLGGSMWRGRIYASHETGVRARRPMIYHGLFGAG
                     LFQGLYTAPSAISPIVLTSFEYHLGITLPLLVLAPLVPLLWPLAALSLIGSIGVCVVA
                     ALQTALPDGKCRVWSKPLVALLFALQPIARGWARYQGRLLSRQTRLEAYETLESLRRE
                     QRQEMPDLLVYRTPSGFDRRAFLSRVVARLTEQGWAHRVDSGWGWFDLELYGGRWCKM
                     RLVTVTDLGSDGQCRMRVRLRPALTLPARVGIGVLLGG"
BASE COUNT         2346 a         4322 c         4932 g         2718 t
ORIGIN      
        1 ggggccgatc cgaagcggac cgggccgcag gggaagacca tactggctgt tgcgcgcgaa
       61 caggggcaca cgtcggtggt cgagtatctc gaagcgcggc tcgcccagaa gaccgagccg
      121 gctggagggc aaccgcgcgg gcgggcgctg atagatgggg gcgtgggtgt gtccgaggtg
      181 ctcgatgggt tctgagcgcg gcgcaacatc aggagagcct ttcctgatga gactgaccgc
      241 cttttttgcc acggtggtgc tgatcccctt cgccgctcat tgcgtctggg gcgcggagcc
      301 tggcaacgat caccgactcg atgagttttt tggagcgtat ctggatcgct ggttcgcctt
      361 gcggcctttg gacgcgaccc gcctgggcga ccatcgtttc gatcatcggc tggatgatct
      421 ctcgcccggg gcgcgcgctc aatggattgc cctgatgcgg gagacgctcg aggcgctgcc
      481 cgagcaggtg gactacaggg cgctgacgcg atcggggcag atcgacttcg agattctacg
      541 ccatgagttg gtcaaatcgc tctggctcgc cgagaacgcg cgtccgtacg aggaagaccc
      601 gcgcatctac aacgactata tcagcgacag cgtgttcctg ttgctgacac agtcgtcact
      661 gcccaaggag accaacatca ccaacgccct cgcgcgcatg gagcaaatcc cgcaggtcat
      721 cgccgcggcg cgggcgaatc tccgcaatcc tccggcggcg cacatcgaga ccgcgattgg
      781 gcagaacagg ggggccatcg cgttttacga acggggtatt ttcgaactgg ccgtgtcgac
      841 gccgcagcgc gcggcgctcg aggccgcggc cgcccgcgtt gcggagagtt tgaggggtta
      901 tcaacgcttc ctggagtccg acctgcgagc gcgggcgcct ggggaatggc ggctgggacg
      961 ggagagattc tgccgcaagc tggagctgga ggtcgatggt ggggtgacgg cggacgaggt
     1021 gctcgccgac gcgcagcgcg aattcgaacg cgtcgaacgc gacatgttca ccgtcgcccg
     1081 tcagctttgg agccgttttc atcccggagc ccccttgccc cccgatgatt ctgcgggtcg
     1141 cagagccgcc gtccggagtg tgttggccgc gattgcgcag gaccacagca ctgcggaaac
     1201 cgttgtggcc gatgtgcgcc gggtgatggc cgacgtcaag cgattcattg ccgacgcccg
     1261 cattgtgcga cttccgcagc cggaccaatg tgagatcatc gaaatgccgg aattccagcg
     1321 cggcaactcg accgcttaca tgaactcgcc gccgccgctt gatccgcggg ctggcggata
     1381 ctacgccgtg agcccgccgc cgcgcgattg ggacgccgac cgtgttcgga gctacttcga
     1441 ggagtacaat tcccagatgc tgcggattct gaccattcac gaggcttatc cgggccacta
     1501 tgttcaactc gaatacgcca atcgcaaccc ttcgctcgtg cggcgagtcc tccagtccgg
     1561 cgtctacatc gagggctggg cggtttacac cgaggcgatg atgctcgatc aaggctttgg
     1621 cgaaggcagt ctgcctctcc ggttgaccca actgaaattc tatcttcgcg cggtggccaa
     1681 cgccatcctc gatcacaaga tgcattgcgc ggacatgagc gacgatgagg cgctgcgatt
     1741 cctcacggaa gacgccttcc agtcagaggg cgaggcgcgc ctcaagatcg tccgcgccaa
     1801 acagagttcc gtgcaactgt ccacctactt tgccggccgc atggcgatgg gccggttgcg
     1861 ccaggcaatc cagcgcgaga tgggcgagcg gttcgcattg gagcgttatc acgaggctgt
     1921 cctcgagccg ggcgccgtcc cggtgaagta cctgcccgag ctggttcgcg cgcggctggg
     1981 tcaaccgcgg tgacgtgttt caggcgcagc ttgcgggcca gccggggggc gcagttagcc
     2041 tgcatttctc atcacttctg gacagcgcga agccggagca cactggagaa gtacaaatgc
     2101 aggcccggat ttcagagtac tccttcaagt ggcgagaaat ccgtatcggg gccgtcagtg
     2161 caggcaagtc tgggcaggga tcaggactcg tctcaggcgc ccgccgctgg tccggttgtg
     2221 atttggagtt ggaaaacggg ctggactgcc gcaccctctg cggcgtgacg aggacattga
     2281 atctggaccc gatcgacgcg ttaatagcgg acattgcccg tgggcggatg gtgattgtgg
     2341 tggatgacgc cgatcgggag aatgaaggcg acttgatcat ggccgccgag catgtgacgc
     2401 cgatggcggt caatttcatg gcgcgatttg cgcgcgggtt gatttgcgtt cccaccactt
     2461 ccgagcgcat cgagcaactc ggaatcaagc aaatggtcac ggagaaccgg gagagtttca
     2521 agaccgactt ccaggtgagc gtggacgccg cccgcgggat caccaccggc atcagtgccg
     2581 cggaccggtg ccgcacgatt caggtgatgg ccgatcccac cgccacgccg acggatttga
     2641 cccagcccgg acacgtcttt ccgctgcgct gcaagcccgg aggggtgttg cagcgcgcgg
     2701 gacacaccga agcagccgtc gatctcgctc tgctggcggg atgtcgcggc atcgctgcga
     2761 tctgcgagat catgaacacc gatggcagca tggcccggct gccggagttg cgcaagttcg
     2821 cccggcgtca ccgcctgcgt ttgggcagca tccaggcgct gatccagtac cgtcgctgcc
     2881 gggagaagct ggtggagcgg atcgagaccg ttcttttgcc gaccgagcat ggcgacttcg
     2941 atttgcatct gtatcgatcg cgcctggatg gccagcatca tgttgccctg gtctgcggcg
     3001 aggtggccgg acagaagaac gtgctggtgc gagtgcacag cgagtgcctg accggggatg
     3061 tcttcggctc ccgccggtgc gactgcggcg tccaattgca ccaggccatg cgccaggtcg
     3121 ccgaggccgg gcgcggcgtg attgtctaca tgcgccagga gggccgcggg atcgggttgg
     3181 ggcccaagat tcaggcctac aagcttcaag agcgcggtta cgacaccgtc gaggccaacc
     3241 acaaactcgg atacgcggcc gacctccggg agtacggact gggcgcgcaa atcctggttg
     3301 acctgggcct caagaccatt cggctgctga ccaataaccc gaagaaactg gtcggtctgg
     3361 aaggctacgg tctggagatc gtcgagcaat tgcccatccg ggtcgcgccc aacccgcata
     3421 atgtcaagta cctgcggacc aagcgcgaga agatggggca cacgctgtga gaatgctccc
     3481 gatgcgccgc cagcacgcag ccaggcaaag ctgcaaccaa agcgccggaa acctcgccta
     3541 tgggtctgtg caagaattag ttccgattga tttttgcaca gactctatga tttcaaaccg
     3601 ggcacgaaga agcccgaaga gtctcaaacg gagcacaacg gttgaaagtc tcccgttgtg
     3661 cgcggaacag aaagacctct actgatgctt cgcaagatca aaacctctca ggccagggcg
     3721 tgcggcggcc gcttcgcgat cgtcgcgtca cgatacaacg cgcgctacgt cgatgccatg
     3781 gtgcgcgcgg cgcgtgccga gttgcggcgc gccggcgtcg gccacgtcga tgtggtgcgt
     3841 gttcccggcg cgttcgaaat cccggtggtg gccgctcgtc tgagtcgcgg cgtcggaatt
     3901 cgccccgacg cagtgatctg cctgggggtg gtgctgcgag gggagaccag ccacgccgag
     3961 cacattggcc ggggcgtcac cgcggcgctg atccagctcc agattcagac cggtgtcccg
     4021 atgattcacg aggtcttggt cctggaagac gaggggcagg cgcgtgtccg ctgtctgagc
     4081 cgtgaccaca atcgcggaac ggaagccgct cagagcgccg tcgagatggt ccgtcttgtg
     4141 cggcatctgc cggcgctgga ggaggacgac gacgtgccgt tctgacccgt atagttcata
     4201 ccccgtccgt gcacagggcg gtcagacatc gcaagaggac tgctatgcgg gtcagaaagg
     4261 taaaaccgcg ccatgttcga gagtcagaga aatccaacac actgttctca tggcagaacg
     4321 ccttcagcac cgacacgaga ctctcggttg tctttctggg agcggtgttg ccggagtccg
     4381 gatgggagac tccgcgcagc ttcatgaaac atccgggctg aagatcgggc tttgattcga
     4441 tgagatcggc ccatagaatc gggcgcatgc aagccgcgcc tgcccgttcc ggcggtttta
     4501 cccggggcca gtggatggtg ttgatcgccg cgttcctggg ctggatgttc gacggtgtcg
     4561 agatgggcat ttttccggtg gttgcgcggc cggcgcttca agagattctt gggacggagg
     4621 cggaccaggc cgtggggcca tggatgggct acatcacggc cctcttcctg gtgggagcgg
     4681 cgggcggggg cctggtcttc ggctggctgg gcgacaaggt ggggcgtgtc cgggcgatga
     4741 cggcgagcat tctcgcctat tcgctgttca cgggcgcgtg ttatttcgcg caggcagcat
     4801 ggcagcttgg tctgctccgt tttctcgccg ctttggggat gggcggcgaa tggtcgcttg
     4861 gtgtggcgct ggtcatggag tgctggcctg aagccaaacg cccattgatg gcggggatca
     4921 tcggcgctgc ggcgaatgtc gggtacgctc tggttggtgt ggtcacctgg ctcattcctg
     4981 tccggcctga ttcctggcgc tgggtgatgc tggccggggc gattccggcc ctgctggcgt
     5041 tcttcctggt tctggtggtt ccggaatccc agcggtggaa ggcatcggtg cagcgcgcgc
     5101 gcgcggaacc gttgcggatt gtgtttggag ccggcctgtg gaggacgacg ctgttggcta
     5161 ttgccttcgg cgcaatagcc ctgattggca cctggggatc agtccaatgg ctgccattgt
     5221 ggggcgacca gcttgtgggc aagagctttc cgcaggtcaa gggtgtcatc atgctggcgc
     5281 tgtcggcggg ggcgattctc ggttgcttca tcgggccctg gattggcgcg gtcctggggc
     5341 ggcgcatggc ctacttcaca ttgtgcgcgt gctccctgct gtcttgtgcc gttctgtttc
     5401 gaatagtcga cagtttcggt ccggcgtttg tcgtcgggtc tttcgtcgtc ggggcggcga
     5461 cggcggcctt ttacgggtgg ctgcctctct acctgcccga gttgtttcct acgcgcgcgc
     5521 gcgcgacggg ccagggcatc agcttcaatg ccgggcggat tctggctgcc ggcggcgcgt
     5581 tgacgcaggg ccagctcgtg tctcattacg gcggcagcta cgcgcaagcg ggggccgtgg
     5641 tgaccctgat ctacgttgtg ggaatggtgt tgatctggtt tggccccgaa accaagggca
     5701 aacccctgcc ggaatgagac tgctctggga tgctgctcga tgaggttgac aaagcgacgg
     5761 ggtgtggtgg acctttgcgg tcgagtgtca cgggaaacgg agagggacgt atgcttgtca
     5821 agatggtcgc catactcgcc gggtgtctga gcatgggcct cgcgttcggc caggaatcaa
     5881 gcacgaacaa gccgcctgaa cgcgtggcag catccgtgca ggcgcccgga tcgattccag
     5941 gcagccgggt cgtcacgtcg atcagccgtc cggcgcccgc agcgccgcgg gccggggtga
     6001 cgatcgacgg ggctattcca aaggcgatcc gcagcaagag cccgctgcgg atgataagcc
     6061 cgttcgcgcc gaaggaatat ggagacggca cggatgtact ggcggttcat ccgatgaccg
     6121 gcgaggcgca gggcgtgacg cttctgaggc tgacatatcc cgatcggcca aaaccgccga
     6181 aatcgcgcaa gagcggaccc ttccggtccc cgagcggcac ataggacgat cgaacgtatt
     6241 ctacacacca tggaactggt aaccgtcttt agcaccttca gccccgccga ggcccaactc
     6301 gtccgctcga ggctcgaggc agccggcata ccggcgaacg tcatccacga gacggcgtcg
     6361 ctctcgatcg agggatacag cgtggcggcc ggcggcatcg aggtccaggt ggcgggcgac
     6421 cggtcggagg aggcgaaggc gctgcttgcc gatgcgcagg aaccgccgcc ggcggcaggt
     6481 gcatgaaggc tgcgctcagg ccgctggctc gggcgctgcc ctccggcgag ttgaccgctg
     6541 atccagccat ctgtcaggcc catgcaggcg acaagtggct tgccagccat gtgccggacg
     6601 cagtggcgct gccacgcagc acggagtcgg tggtctcgat tctgtccttt gcccaccgcc
     6661 gaggcatccc catcactgcg cgcggagcgg ggcacggcta cgtgggcggg tgcgtgcctg
     6721 tgcaaggagg catcgtgctg tcgctggccc ggctgaatcg cattcgcgag attcacccaa
     6781 gggacttcgt ggccgtagtg caatcggggg tcatcacgca gaagctccag gatacggtcg
     6841 agcgcagggg gttgttctac ccgcccgatc ctgccagtcg ctcggaatgc agcgtgggcg
     6901 ggaatatcgc gaccaacgcg ggcgggccga ggtgtctgaa gtacggcgtg acacgggatt
     6961 acgtcctggg cctcgaagtg gtgctggcgg acggaatcgt cgtgcgactc ggaagccgca
     7021 cgcacaagaa caagaccgga ttcgatctcg cccggttttt tgtcggatcg gaaggtcttc
     7081 tgggcgttgt gacggaagcg accctcaagc tgttgccgct gccaccctac cgcgccgctc
     7141 tcgcggcggg cttcggcacg acgcgggacg cctcccgcgc cctccagtct gtctttcggg
     7201 ccggctttct tccctgcgcg ctcgaagtgg cggatgcatt cactctcgcc gcggcggcga
     7261 aacgcaccgg cagccgccgg ttggccgggt gcgaggccca cctcatcgct gaactggacg
     7321 ggcaatcccg gtcagtgcga tccgagctgc gcgaggtcga gcgggtcatc gggcagcacc
     7381 gcccgcgctt tatcgagcgg gctttgggtg cgatcgagtg cgagcggatc tggcagttgc
     7441 gccgcgagtt ctcctacgcc ctgcgtgaca ccggattgac caagctcaac gaggatatcg
     7501 ccgtgccgcg cagccggctc gaggccctgt tcgcctttgc agcacgtctc cagagaaaac
     7561 acggcttgcc tgtggcctgc tttggccatg ccggcgacgg caatatccac accaacatca
     7621 tggttgaccg gacacagccg ggggcggaga cgcggagtcg agccgctctg gatgatttgt
     7681 tcaggcaagt cctggcctgg ggcggcgtga tcacgggcga gcacggtgtc ggcttggcaa
     7741 agatgccgtg gtggaaccag gcggtttcac ccgaagttcg gcaactccac gctgccatca
     7801 agcgcgccct ggaccccaag ggcattctca atccgggcaa gttcgtctca cttggtgcgg
     7861 gcgatccctc ccaccgccag cgggcgtggt aagggtggat tatccgcaaa cgcgtggcgg
     7921 cgattgtgcg gacaaggccg ggagactgga ggacttttct tcggagcgga ccccggcccg
     7981 ggtgtttcaa gacacttcgc ggagtctgat gtccggccac gcacgcggat gctgccgacc
     8041 cgatcggcct gtcgtcacgg cgcctcgagg ccgcgcgccg cctgggcgag ggcccgggcg
     8101 cgatgactga tcctgttctt gaccggctct cccaactcgg caaacgactc cgtgtatccg
     8161 agcggttgga agaccgggtc gtagccaaag ccgttcgcgc cgcgcgccgc gtcgagaatc
     8221 aggccttcgc agacgccttc gaaaagccga ggcgggcggg tctgcccgac cgtggccagg
     8281 gcgatcacgc agcggaatcg ggcggtccgg tttggggtgg agacgccgtg cagcaaccgc
     8341 agaagcttgg ccgtgttgtc gatgtcggtc gcgttggctg acggcgcgct gtccaaagct
     8401 gcgaagcggg ctgagaccac gcccggcgcc ccgtcgagcg catccacttc aagcccggaa
     8461 tcgtcggcca ggacgcagag cgcgccgcct tcgcgcgcga tcggctgctc ggccagccat
     8521 cgcgccagcg aatcggcctt cttcgccgcg ttcccggcga atgtgtcggc gtcctcaacc
     8581 gtggtcggcg cgccggggaa gtcgttgagc gtcaggtagc gcaacccgtc gcccagcacc
     8641 gcccgaatct cccgggcttt gtgatggttg cgcgtggcga tgaccagcgt cgtcatgggc
     8701 gaacagtgtg ctcgaactgc tcgccaaaca agagctcaaa gcgttcgagg cacccatgct
     8761 gatcgccctt ccgacaactg ttcaaggtcc ctgcgcgcat tgacgcgcgc attgacgcgt
     8821 tgagtttccg attctcgttt cgatgacttt ccagtagggt cggcctctcg gcatgcgaac
     8881 ctcttccgca aaagacgagt ccggcgcgag atcagacctc gcggagcact gggtggcatt
     8941 cggggcgggg tcgcgcgggc cggagtgtcc cggctgcgcg aggcgcccgg acaaccatcg
     9001 acgagcgtct gtgttggcgt ctttggcttg gattgtggcg tttgcatggg cgggtttgtt
     9061 ccgtcatgga ttggccgggg cgcctgaggt ccagccccag acgttgatcg aatggacgtt
     9121 cgaccgcgag ggtgatcagg ccggatggca cgccaacgac cattttgcag atgttcgggt
     9181 cgtggggggc gcgctcgaat gccggggcgc cggaacggac ccgattctcg agctggggtt
     9241 gccgctcgaa gttgcggtct cacaccgaca ggctctcgaa gtgcggctca aggccagtcg
     9301 ggacggaacg gcggagtttt tctggagcaa cacgactcga gggcgatacg gcgggttcag
     9361 ccaggagaag accacgcgtt ttccggttgt cggggacaat gcatgggcga cgtaccgggt
     9421 gttgcctggc tggcaggccg agggtcggat cattcgtttg cgtctggacc tgtacgacaa
     9481 cgccgagttc gcgatcgatg ccattcggat cgtctcgtgg cccgagttgc aaccggttga
     9541 cactcgcgag ttcgagtttc cggccgcgga ccgtgactgg ccgtggattc ggatgggacg
     9601 catcctgtcg tcggcctcgc cattggcggg tggcgatcga ttgcgtctgg gcccgacgct
     9661 gtcgatcgac gccgaggcgc tcgattacgt ctcgatgtgc atgacggttc cctcgcaacc
     9721 cgaggctggg cgacgcgagg cgtctcccgg aacgcccctc gctgcgccgc gcctgacgct
     9781 ctggtttgcc ggcaaacagg attacgggtt caagagcgtc gctgttcccg tccatgccga
     9841 tggacagaag cacgtctacg acgtggacat gctggtcgcc aacgggtggc gcgggcagat
     9901 tgtggcgctc gggatcgagc acacgctcga tgttgccggg caaccggagt tgcactggct
     9961 taaaattgcc gatcgtcccc agggcccggc gcgcttgagc gtggtttcgc tcggcctcga
    10021 gccggcgttg ccgcgggtgg gaaagcctct gagactcgaa gccgtgttgg ccaaccacgg
    10081 cggcgccgcg gctcgcgcgg tcactggaga tctgatcctt cccgatggac ttgcagtccg
    10141 gggcggggac gtggcgggtc ggagcacgcc gcagctcggt ccgggggaga cggccacttg
    10201 ggagtgggct ctcgtcgggg accggccagt gcaggggcag atcgagttgc gtctgcgtgc
    10261 ggaccaggcc gagccggccc aggcccggat ggcggtggct ttgacagagc ctgtggacat
    10321 tggtccgtgc gattacgtgc ctgaacccag accggtgcgt ggaccttacg aggtgggcgt
    10381 gtactacttc ccgggctgga agagcgccgg ccaatggcat ccgatccggc cttatccgga
    10441 gcggcgtccg gtgctgggat ggtatcggga aggcgatccg gaggttgcgg actggcacat
    10501 caagtgggct gtcgagcatg ggatcacctt cttcgcctat gattggtatt ggtcgcaagg
    10561 ggcgcggcag ctcgaacacg cgctgcacga cggttacttc aaggcacggt accgccgcta
    10621 cctcaagttc tgtctgctct gggcgaacca caacgcgccc gggacatcct cgcacgccga
    10681 ctgcgtggcg gtcacccgcc attggatcga acactatttc agacaacccg agcacttgac
    10741 cgtcgaaggc aagccgctgg tgattatctt cagtccggat cgtctctctg ccgacctcgg
    10801 atcgaccggc gtcaagtcgg catttgacgc catgcgcgca acctgccgcg aggccggctt
    10861 tcccggattg ctgcttgttg cctgtgtggg cgacgcggcc caggcccgcc aggctgccgc
    10921 cgagggttac gacgcggtca cagcttataa ttggcctgga ctcgatatgt ctgccggcca
    10981 gatggaagcg ccctacgccg cgcttctccc cgcctatcgg cgcaattggc agcatatcat
    11041 cgattcggcc ggcatccccc tcctgattcc gctctgtgga ggttgggaca gccggccctg
    11101 gcacggggcc aacaacctcg tgcgctatgg ccgcacgccc gagttgttcc gccagcacgc
    11161 ccaggacgcc gcccaactct cgagacgcac cgctcacctc tcgccgctct cgctcctgct
    11221 gctcgtcgag gcctggaatg aattcggcga aggctcctat atcgagccgc atcaggaatt
    11281 cggattcggg tatctggacg ccttgcgcga cgtgttcacc gccgcgccga gggaccatgt
    11341 cgatgtgacg cccccggacg tggggcgagg gccttacgat gttgcggtgc atacgatgcc
    11401 tgagcccgtg tgggagttcc gcgagagcga tgagggatgg agtcagacga tggatctgac
    11461 cgacgtgcgg atcgaaaacg gcgccctgat cgcgcgcagc accggcaacg atccggcctt
    11521 gttcggtccg cccattcagg cgcgcgccga cgcgtatcga accgcggttg tccgaatgaa
    11581 actgacgcct cgcgatgggc ggtctctgcc ggaccgggct caactgttct ggcgcacgga
    11641 tcggttgccg gagggcgagg ccagcagcga gcggttcgat gtgctggcgg acggcgcgtg
    11701 gcacgagtat cgggtggatc tcagtggaaa ccgccggtgg cgcgggatca tcacccggct
    11761 gcggctcgat ccgtgcaatc agcgggacac acaaatcgct ctcgatgtgc tgcggctcga
    11821 gccgtgaggc gccaatgaag cggccttcct gaccgatctg gcccggggaa ggagagtctg
    11881 agtcgtttga tattccacgt tggcgcccac aagagcttcg gcaatgctct cgcaatgctc
    11941 tgccaacact gaatccgacg ccacaggtcc tcgtctaaga gcgcaatgcc ggatgctgag
    12001 tcacaccgcg cccgagtggt ggtggatggc aagtttttcc gcctcgggcg agacaagttc
    12061 ttcgcacgcg gcgtgaccta cgggccattt gcgccgaacg ctgccggcga gcacttctgc
    12121 tcgccgacgc agacggcgca tgatttcgac cagatgcgcg cgtgggggat caatttgctg
    12181 cgcgtatacg atgtgccgcc gggctggttc ctcgagttgg ctgctttgaa agggctgtgg
    12241 ttgctggtgg atgtgccttg gaacaagcag gcctgcctgg acgatgcgcc ggccagggag
    12301 gcggctcggc gggctgtgcg ggaggcggcg ctggcctgcg cggggcatcg ggcggtgttc
    12361 gggatcagcg tggtgaacga gctgccgccc gacagcgttc gctggagcgg cccggcgtcg
    12421 atcggttcgt ttttggagga gttggtgggc gtggtcaaga gcgtcgacgc tcagtgcctc
    12481 tgcacgttcg ggagttttcc cccgacagaa tacctccggg tgcaggcgat cgacttcgtt
    12541 tgtttcaacg tctatctcca tcaccctcag gcattcagaa actacctcgc ccgcctccag
    12601 gtgttggccg attccaagcc cctggtgctc ggcgagttcg gactcgactc ccttcgggag
    12661 ggcgagccgc gccaagcgga gctgctcgaa tcgagcatcg agagcgtctt tcaggccggg
    12721 gctgctggtg cggtggtttt cagcttcacg gacgcgtggt tcaaagacgg actggccatc
    12781 gaggactggc atttcggact gacaagtgcg gcgcgggtcc ccaaacccgc ggcgttcgcg
    12841 gtgcagaggg cctacggggg cgcaccgcat ttccggaccg tctccggccc gatggtctcg
    12901 gtggtggtgg ccagttacaa cggggcgcgc accttgagcg cctgcctgga atcgctcggc
    12961 gccctctctt accctgtctg cgaagtcatt ctggtcgatg acggctcgac cgacgagact
    13021 ccggagatcg cggcccgctt cccacgggtg cgctatttgc gccacccgca aaacctcggg
    13081 ttgtcggtgg cgcgcaacac cggcatcgag gcggcgcagg gtgagatcgt cgcgttcact
    13141 gacgccgact gccgtgtcga cgaagactgg ctgcattacc tggtgagcga tttggtgggg
    13201 agccggttcg tcgggatggg cggtccgaac ctgctgcctt cggaggactc ctgggtggcg
    13261 gcggcggtgc aggtatcgcc gggaggcccc gcgcatgtgc tgctgacgga ccaggttgcg
    13321 gagcacatcc ccggctgcaa catggtcttc tggaagtggg ctctcgagga aattggctgt
    13381 ttcgacccgg tgtttcggaa ggcgggcgac gacgtcgaca tctgctggcg tttgcagcag
    13441 cggggctatg agatcggatt cagtccggcg gcggtggtct ggcattaccg gagatcgacc
    13501 gtgcgggcgt atctgaggca gcagcacggt tacggcgaag ccgaggccct tctcgaacgt
    13561 aagcaccccg agcatttcag tccgctcgga ggcagcatgt ggcgcgggcg catctacgca
    13621 tcgcacgaga cgggcgtccg ggcgcgccgc ccgatgatct atcacggcct gttcggagcg
    13681 ggcctcttcc agggcctcta caccgcgccg tcagcgattt cgccgatcgt tctgaccagt
    13741 ttcgagtacc atttggggat caccctcccg cttcttgtct tggcgccgtt ggtcccgttg
    13801 ctttggccgc tcgcggcgct tagcctgatc gggtcgattg gcgtctgtgt ggtcgccgcg
    13861 ttgcagacgg ctttgccgga cggaaagtgc cgtgtctggt ccaaacctct ggtcgcgctc
    13921 ctgttcgcgc ttcagccgat cgcgcggggc tgggcccggt atcaggggcg tctcctctcc
    13981 aggcagacgc ggttggaggc ctacgagact ctggaatcgc tcaggcgtga gcaacgccag
    14041 gagatgccgg atctcctcgt ctaccgcact ccgtcgggat tcgatcgccg ggcgtttctg
    14101 tcgcgggttg tggcgcgtct gaccgaacag gggtgggccc accgggttga cagcggctgg
    14161 ggttggttcg atctggaact ctacggcggg cgatggtgca agatgcgcct ggtgaccgtg
    14221 accgatctgg gttcggacgg ccaatgcagg atgcgtgttc ggttgcgtcc ggcgctcacg
    14281 ctgccggctc gggttggcat tggggtgctt ctgggcgg
//
LOCUS       DAOQRO010000036        14165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3349_length_14165_cov_4.990999, whole genome shotgun sequence.
ACCESSION   DAOQRO010000036 DAOQRO010000000
VERSION     DAOQRO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_14165_cov_4.990999"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1351
                     /locus_tag="PKK20_02525"
     CDS             344..1351
                     /locus_tag="PKK20_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98794.1"
                     /translation="MSATRFWLGLGMAVLAAGPLSAAGSVAETPAVPDSAATAAADAV
                     EKAFQALIADDDAAQAEVDRWIQETKTDDSQGPDLSGITLKARIEQRLEPVRRAYERF
                     VQRHPDHVRARLAFGSFLNDISLDDEALVQWKKARDLDPNNPAAWNNLADYYSRHGPT
                     RLAFECLDEAIRIKPSEAVYQRNLATLVFLYRNEARIHYRLADEQQVLRRTLELYQKA
                     RRFDPNNFQLATDLAQVYYYLKPEKVENPARLKAAGDKLTDEALRAWLEARALARTDL
                     DREGVAVHLARVCLRAGRLDQARQYLGEIKNSSLAAGKEALEQTLQKLEKGEPVPLSE
                     L"
     gene            complement(1530..3212)
                     /locus_tag="PKK20_02530"
     CDS             complement(1530..3212)
                     /locus_tag="PKK20_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNU98795.1"
                     /translation="MLTMEEALRQSPKTFAAGQIVKGTILELRPKEVLVDIGYKSEGV
                     ISLAEFEDPKAARIGEEIDVLIERLEDREGMVVLSREKAEFKKNWERILSICNEGGTI
                     EGKVKSIVKGGLLVNIGVEAFLPASQIDIIPPRNLSGFVGNTYRFKVVKINQERQNIV
                     LSRRELIEQERSERRAKLLAEMMPGDIRKGTVKNLTDFGAFVDLNGLDGLLHITDMSW
                     GRIGHPSEMLKVGQELDVVVLDINREKERVSLGLKQKMANPWDTIESKFPIGAKVKGK
                     VVNLVPYGAFVELEPGVEGLVHVTELSWTKRIAKASDVLKQDQEIEAVVLGINREEQK
                     ISLGVRQLETNPWEQALEKYPKGTRVKGKIRNLTSYGAFLELEEGLDGMIHVSDISWT
                     RKINHPSEVLKKGEEVEAVVLEVDKVNQRISLGVKQLAEDPWENIDKYYRVGDLVIGK
                     VTKLASFGAFIGLEHDIDGLVHISQVSEERIDKIKNVLAVGQEVTSRVIKIDKGERRI
                     GLSIKAANYSPEQLKAEQALLDALKPGEDLVALQHAFDAADEARQSDDNGKP"
     gene            3509..3593
                     /locus_tag="PKK20_02535"
     tRNA            3509..3593
                     /locus_tag="PKK20_02535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3543..3545,aa:Leu,seq:gag)
     gene            4276..6762
                     /locus_tag="PKK20_02540"
     CDS             4276..6762
                     /locus_tag="PKK20_02540"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNU98796.1"
                     /translation="MLRRSQRLFGLLSLAALNLSAPGVLGGEAELKLPPLDTVHFPAL
                     GGASGYTLMLLGIVVCALAAVFGIWQYNHTKTLPVHDSMRNVSSLIWETCKSYLFQQG
                     KFLAILWVLIGVCIAYYFGFLTQHQDAAGTVTKGMPVGKVVVILLASILGILGSYGVA
                     WFGMRINTQANSRSAFAALTGLPWGPLAIPMRSGMSVGMLLITVELFFMICILIFLPA
                     DLTGPCFIGFAIGESLGAAALRICGGIFTKIADIGSDLMKIVFKLPEDDPKNPGVIAD
                     CTGDNAGDSVGPTADGFETYGVTGVALITFLALTLGLTPLCATLITWIFVMRILMIIT
                     SFLAYVITNKVFTTKYSQAKDFHPELPLTWLVWLTSIISIVVTFVISNLMLKDLQVNG
                     TAVPDLWWVLAVIISCGTAAGALIPEFTKIFTSTEARHVKEIVTASKQGGASLNILSG
                     FVAGNFSAFWQGLLMLILMLIAYLMTLPAISPALSQTMPAAISFAAPIFAFGLVAFGF
                     LGMGPVTIAVDSFGPVSDNAQSVYELSQIESIPGIDKDIEKNFGFKPDFKAAKHNLEK
                     NDGAGNTFKATAKPVLIGTAVVGATTMLFAIILLLDKEFGGVITKLSLVQPTILLGFL
                     MGGAVIYWFTGASCQAVVTGAYRAVVYIKENMDLSAKTASIEDSKEVVKICTIYAQKG
                     MFNIFVAIFSLSLALAFFDSYFFIGYLISIAFFGLYQAIFMANGGGAWDNAKKIVEVD
                     LRQKGTELHDATVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLATEIAVAMQKAQQTG
                     LKTGIAVVLFVVALVFVYRSFYGMRIPEEK"
     gene            6928..8661
                     /locus_tag="PKK20_02545"
     CDS             6928..8661
                     /locus_tag="PKK20_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU98797.1"
                     /translation="MCGPAGRNIAFLAIAAVLIGCGTRHYRKSADKDVYRIIEQVEAQ
                     VFKRTNAFTIDTPYSTRDPKTILPAEIVEDRMATNRRVINLRDALDLAVRKSREYQNQ
                     KEQLYLRALALTGQRHRFTPNFFASVNPSASGDGNGNVDLDTLKPEVGFSQVFKSGGS
                     LSVRLVNDLMRYYTGDPGTAALSVMSVDIAQPLLRGFGRNSDAVEALTQAQRSLIYSI
                     RSFDQYQRQFAVGIVNNYFGLLGQKDSVRNNYTNYLRRVETRRYTEARAVDRVRQSEV
                     DDARTQELNARIMYVNSLAQYMSQLDAFKVTLGLPLKEDLYLDDGDLRELSSAGLGAV
                     PIDRDAAFRIAIAKHADILNAIDSFEDAKRRVRLAADKLKPGVRFQGSASYAVQDSTN
                     SWSYHYTRFNPDQFRYNFGLQIDLPLDRLAERNNYRGTLVDFERELRQLSLTLDNFKE
                     RIDRGLRTLEQRRLNYISQLDALEVARRRVENDTLLLEAGRTDVQRLRQSQDALIQAQ
                     NDLVFTMVDHLQARLQVLLDLGVITTDHPSFWLQDPLTAALDESQLGPPPLQMPDDNP
                     IPPNQFLEPSS"
     gene            8658..10676
                     /locus_tag="PKK20_02550"
     CDS             8658..10676
                     /locus_tag="PKK20_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU98798.1"
                     /translation="MNASASPDSAAPPRARRRLFQVGAVGLLLLLLYVVLRPSKKEGG
                     GTEHYLVKRGDFTVSVIEPGTLTAISEVVIRSEVEGTARIISIVPEGASVKKGDLLVE
                     LDSSQALDQVNQQLISVEKARFAVTNAEAQLDIQRSATNVDYQAAKLKLDLAIIDRDK
                     YEQGQRLVDLLEAQNKALQASNQLVVNRETYSNSVMLAKKGYETKQKVDGDRLSVLAT
                     ENQLIAATNVLWMLDQFDVEKQIKKFNSDVQQAEEQLQQQINLNKRKMAQYEADLLSQ
                     INTLKLNEDKLERDRKNLDATMIYAPQDGLVVYQVSENRFSSESLIEGGAVVRNRQEL
                     IKLPDLSRMKVVVKIHESHINMIRAGLPAFVVLDSDPDKRYAGVVAKVAPLPDTQARW
                     GNPNLKVYNTEVYLSENPPNVKPGVSAKAEIVITNIADVLSVPIQAVTTFKGKQVVYV
                     LNRGKPEPRPVEVGMFNTKFIQIVQGLEAGDRVMLSPPFDSHEKDLEGAVLADEEKAR
                     ITFTNVPPIEAAPHNGLGNQANSRGGANGRPALTAAGTEGGGRGPTPGAGAGSGPLGG
                     VIPEEILKQYDKNGDGTLDETERESMRAAFASRFGGQGSPAGGPGGGMRPGREEMMKR
                     FDKDGDGQLDEEERAAMREAMGSLRPNRGEGEAGARPGRDAAPSPRQP"
     gene            10680..11411
                     /locus_tag="PKK20_02555"
     CDS             10680..11411
                     /locus_tag="PKK20_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98799.1"
                     /translation="MPADSAIIRIRDVEKRYVLGGEADVLALRGVNLDVEAGSYLAIM
                     GPSGSGKSTMLNILGCLDRPTAGRYFLGGEDVSRMPDDQLSEARGLKIGFIFQSYNLI
                     AQLTVLENIQVPLLYQGKDVRTHLESCLELAKLVGLGDRLHHRPSQLSGGQQQRVAVA
                     RSLVNNPLMILADEPTGNLDSKTGQEVLDLIDRLNEAGKTIVLVTHDDRVASRADRVI
                     HMKDGLIDREVVNRPMRARPAVSSS"
     gene            11427..12719
                     /locus_tag="PKK20_02560"
     CDS             11427..12719
                     /locus_tag="PKK20_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU98800.1"
                     /translation="MLTFQFVSTVRLGIKSLMLHKLRSALTMLGIIFGVCSVIAMLAI
                     GEGASYEAQEAIKNLGSNNILLRSLKPPDQARQQAGGSPRGMQLAYGLTYEDGARIQS
                     TIPGVNRVLPMRIIRENVRFDRNEIPCQVIGTLPSYMAVTGAGIVRGRFLAHLDEQHA
                     ENVCVITTGLARRLFPHLDPMQHAVKIDTFYYRIIGILQERSQPEQRTQAGAMEGQPL
                     DNNVYIPLSVSRTRFGETLIRRSAGSFEAEEVQLHQITVQMQNLASVETADPQIRTLL
                     GRFHDQNDYEIIVPLQLLRQAEQTKRIFNIVLGSIAAISLLVGGIGIMNIMLATVTER
                     TREIGIRRALGAKRRDITTQFLVETVVLSVGGGLVGVLVGVLTPLVVSQLTTMKTIVT
                     VWSVLLAFGISGAVGIVFGLYPASAAAKLDPIEALRHE"
     gene            13091..>14165
                     /locus_tag="PKK20_02565"
     CDS             13091..>14165
                     /locus_tag="PKK20_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98801.1"
                     /translation="MPSYDDVLVVVKQGDAESAAAAQYFKEQRRIPDENLVTLPLTGA
                     IPTVAARDAFIGGIKAHMAQNGLQGRINYIVLSSNFPRECKDTATAPYNAIFDAYLMW
                     ALSDTYPSTSYLDCIPLHNNPYYYIRKSNYEDRTAIKFTKTKYRYYIVSRLDGEGLAA
                     TKQLIDHSGHVAYDQYKKGINFLVDDDPSNWEVYRYQAERAELKERGINIIDWNPGVS
                     GSIHNLHGLSFAYFNWVNGTSDPFISYRNISKHMRFNPGALVFAYRSYPARLFKRPLC
                     GLSQCNVNTRAVQSLMKSDASDLVLRQMSDVAYATENNSVWCALGQDILKMYATANAR
                     SANNFRGNGVAVYAPDGTLLNHFT"
BASE COUNT         2708 a         4352 c         4298 g         2807 t
ORIGIN      
        1 acttcgacgc ttgccgcggc gtggttgaga atgaaggtga ccgtgtcgcc cgtccgcttc
       61 acgccggacg cgtaagggat ggccagggcg gtcgaggccg cgaacgcaag gcccagcgcc
      121 agcgccaggg ctggtgtgag gagggtgttc atgcaggcat gggtttggat ggaactcata
      181 tcggttcgtc tcgatttcga ttgcaggttc aactcaattc gacccgcacg tttcgcttca
      241 ctgactttca ggtccgatta ttcaaggccc atgcctaaac ccggatgccg ctgagggcaa
      301 accttttgtt cccagccggg ctcgattccg gtattgttcg ggcgtgagcg cgactcgatt
      361 ctggcttggt ctggggatgg cggttctggc ggccggaccg ctgagcgccg ccggcagtgt
      421 cgccgaaacc ccggcggtgc cggactcggc cgcgacggcg gccgcggatg ctgtcgagaa
      481 ggcattccag gcactgattg cggacgacga tgcggctcaa gcggaggtgg accggtggat
      541 tcaagagacc aagaccgacg attcgcaggg tcctgatctg agcggtatca cgctcaaggc
      601 gcgcatcgag cagcggctcg aaccggtgcg ccgggcctac gaacgcttcg tgcagaggca
      661 tccagaccac gtccgggcac ggcttgcgtt tggcagtttt ctcaacgaca tcagcctgga
      721 tgatgaagcc cttgtgcagt ggaagaaggc acgcgacctc gatcccaaca acccggcggc
      781 atggaacaac ctggcggact actacagtcg gcatggcccc acgcgcctcg ccttcgagtg
      841 tttggatgaa gcgattcgaa tcaagccgtc tgaggccgtc taccagcgca acctggccac
      901 gctcgtgttc ctctaccgca acgaggctcg aatccattac cggctcgcgg acgagcaaca
      961 ggttcttcgc cgaacgctcg aactctatca gaaggcgcgt cggttcgatc cgaacaattt
     1021 ccagctcgcg actgatttgg cgcaggtcta ctactatctc aagcccgaga aggttgaaaa
     1081 tcccgcgcgt ctcaaggccg cgggagacaa gttgaccgat gaggcgcttc gtgcatggct
     1141 cgaggcccgg gccctggccc gcaccgatct ggaccgtgag ggcgtcgcgg tgcacctggc
     1201 gcgcgtctgt ctcagagcgg ggcgtctgga ccaagcccgc caataccttg gcgagatcaa
     1261 gaacagcagt ctggctgccg gcaaggaggc gctcgaacag accctccaga aactcgagaa
     1321 gggcgaaccg gtccctctgt cggagcttta gggtctgtgc aaagcttccc gtgcctgccg
     1381 tacccgtcgc ggagcgcgaa atcaatccgc gccctgcgac ggcagaaacg gaaaggccgg
     1441 gccgcgaagg cccggccctg agtcgtgttt cagtgcctga ggcgtcgacc gcaccggaag
     1501 gggaggtcgg acgcgtcggt cggggttggt tacggtttgc cgttgtcgtc cgattgccga
     1561 gcctcgtcgg cggcgtcgaa cgcgtgctgg agggcgacca ggtcctcgcc cggtttaagc
     1621 gcatcgagca gggcctgctc ggccttgagt tgttcgggcg agtagttggc ggccttgatg
     1681 gagaggccga tgcggcgttc gcccttgtcg attttgatga cgcgggaggt gacttcctgg
     1741 ccgacggcga gcacgttctt gattttgtcg atgcgctcct cgctgacctg ggagatatgg
     1801 acaaggccat cgatgtcgtg ttcgaggccg atgaaggcgc cgaagctggc gagcttggtg
     1861 accttgccga tgacgaggtc gccgacacgg tagtacttgt cgatgttctc ccaggggtct
     1921 tcggccagtt gtttgacgcc gagcgagatg cgctggttga ccttgtcgac ttcgagcacc
     1981 acggcttcga cttcctctcc cttcttgagc acttcggacg ggtgattgat cttccgtgtc
     2041 catgagatgt cggagacgtg gatcatgccg tcgaggcctt cttcgagttc gaggaaggcg
     2101 ccgtagctgg tcaggttgcg aatcttgccc ttgacgcgcg tgcccttggg gtatttttcg
     2161 agggcctgct cccacgggtt ggtctcgagc tggcgcacgc ccagcgagat tttctgctcc
     2221 tcgcggttga tgccgagcac gaccgcctcg atttcctgat cctgcttgag gacgtcgctc
     2281 gccttggcga tccgtttggt ccacgacaat tcggtgacgt gcacgaggcc ttccacaccc
     2341 ggttcgagtt cgacaaacgc gccgtacggg accaggttga cgaccttgcc tttgactttg
     2401 gcgccgatgg ggaacttgga ctcgatggtg tcccaggggt tggccatctt ctgtttgagg
     2461 ccgaggctga cgcgctcctt ttcgcggttg atgtctagca cgaccacatc cagttcctgg
     2521 cccaccttga gcatttcgct cggatgcccg atccggcccc agctcatatc ggtgatatga
     2581 agcaagccgt cgagcccgtt caggtcgacg aaggcgccga agtcggtcag attcttgacc
     2641 gtgcccttgc gaatatcgcc cggcatcatt tcggccagca gcttggcgcg gcgctcgctg
     2701 cgctcctgct cgatcaattc cctgcgggac agcacgatgt tctgccgttc ctggttgatc
     2761 ttgacgacct tgaatcggta cgtgttcccc acgaacccgg agaggttgcg gggcgggatg
     2821 atgtcgatct gcgaggcggg caggaatgcc tcgacaccga tattgaccag caggccgcct
     2881 ttgacgatgg acttgacctt gccttcgatc gtgccgcctt cgttgcagat cgagaggatt
     2941 cgttcccaat tcttcttgaa ctcggccttc tcgcgggaga ggaccaccat gccctcacgg
     3001 tcctcgagcc tttcgatgag cacgtcaatc tcctccccga tccgggcggc ctttgggtct
     3061 tcgaattcgg cgagcgagat gaccccttcg ctcttgtagc cgatgtcgac gaggacttct
     3121 ttggggcgta gttcgaggat ggtgcctttg acgatttggc ctgcggcaaa ggtcttggga
     3181 ctttgcctca gggcttcttc catggtgagc atacggattc tgtcgtgtaa acgcgacgcg
     3241 actgggggag acttggtcgg gaacaaatga cccggcgaag actccattgc ctaacggacc
     3301 aaacgttgcg gtggcaacgg aggttgaagt caggttgttt cgttaacagt tcttttctca
     3361 gcctcatgcg aacaccgctt cggcggcgct caggcggggg cagagcatag gagcgcccct
     3421 tccggatgca agcggatttg cgtagaattt gcgcagaaaa gaacttgcag ccgccgggcg
     3481 gtctggcttt attaccccag ccgcggttgc ggatatggtg gaattggcag acacgctact
     3541 ttgaggtggt agtgccgcaa ggcgtgcagg ttcaagtcct gctatccgca ccacttttcc
     3601 tgaggctatc ggggtgtctc cagtgtttcc cgcacgcgtt cacatcccga caatcactgc
     3661 cggaccagga cttgtccctg catcgggctg gcgcggcgcg atcctctccg ccgtgctcgg
     3721 gctctcgctc tggatgcacg tcgcggattc ggggcttgtc ccactggaac gcgactcgcg
     3781 gtgcgggtct ggcgcaagga gcacggtctg cccgatggcc gggtgccggc gttgctgcgg
     3841 gacggagtcc ggatcggcat gggcggtggc ggcctggcgc ggctcacggg ttcgtcctca
     3901 ggtcgtttcg ccgcgagggg tcgacccggg aagggcgtga gcggctgagc cctcgagaga
     3961 tcgaagtgct cgaggcgctg gcccaaggga tgagctacga ggaaatcgcc gctcaccagg
     4021 gtatcagccc ccggaccgtc aacaccctcc ttcaccgcat ctacgcgaaa ctccacgtcc
     4081 attccgccgc cgccgcggtg ggcaaatggc tttcgaggcg cgattcgacc ctttgtgaac
     4141 cttgtggggg ctgtgaatcc gcttgactca ttgcgggcaa tctcatagtt gcgagcgttt
     4201 caagccggtt ttgatgaacc ggattaatgg tggtttgccc ctgtcgggcg gacgccgaac
     4261 cctaaaccga acactatgtt gagacgaagc caaagactgt tcgggctgct gtccctggcc
     4321 gccctgaatc tgagtgcccc cggcgtgctg gggggtgaag ccgagctgaa actgccaccg
     4381 ttggatacgg tgcacttccc ggctcttgga ggggccagcg gctacacgtt gatgctgctg
     4441 gggattgttg tgtgcgcgct ggcggccgtt ttcgggattt ggcaatacaa ccacaccaag
     4501 actcttccgg tgcatgactc gatgcggaat gtgtcgagcc tcatctggga aacctgtaag
     4561 agctatctgt tccagcaggg caagttcctg gccatcctct gggtgctgat cggggtctgc
     4621 atcgcctact atttcgggtt cctcacccag catcaggatg ccgcgggcac cgtgaccaaa
     4681 ggcatgcccg tcggcaaggt cgtggtcatc cttctggcgt cgatcctcgg cattctcggt
     4741 tcttacggcg tggcctggtt cggcatgcgc atcaacaccc aggccaatag ccggtccgcc
     4801 tttgcggcgc tgacgggtct gccatggggg ccgctggcca ttccgatgcg ctccggcatg
     4861 agcgtcggga tgttgctcat cacggtcgag ctgtttttca tgatttgcat cctgatcttc
     4921 ctgcccgccg acctgaccgg tccatgcttc atcggatttg ccatcggcga atcgctgggc
     4981 gcggcagcgc tgcgcatctg cggcggcatc ttcaccaaga ttgccgatat cggctcggac
     5041 ctgatgaaga tcgttttcaa actcccggag gacgacccga agaaccccgg ggtgattgcc
     5101 gattgcactg gcgacaacgc gggcgactcg gtagggccga cggctgacgg cttcgagacg
     5161 tatggcgtga ccggtgtggc gttgatcact tttctggcgt tgacgctggg cctgacgccg
     5221 ctgtgcgcga ctttgatcac gtggattttc gtcatgcgca tcctgatgat catcacgtcg
     5281 ttcctggcct atgtgatcac gaacaaggtg ttcacgacca aatacagcca ggccaaggac
     5341 ttccatcccg aactgccctt gacgtggctg gtctggctca cgtcgatcat ctcgattgtg
     5401 gtcaccttcg tcatcagcaa cctcatgctc aaggacctgc aggtcaacgg cacggcggtt
     5461 cccgatctgt ggtgggtgtt ggcagtcatc atctcctgcg gcacggcggc gggcgcgctc
     5521 atccccgagt tcaccaagat tttcaccagc accgaggcgc ggcatgtcaa agagatcgtg
     5581 accgcatcca agcagggcgg cgcctcgctg aacattcttt ccggattcgt ggccggcaat
     5641 ttctccgcct tttggcaggg cttgctcatg ctgattctga tgctcattgc ctacctgatg
     5701 acgctgcccg cgatcagtcc tgcgctctcc cagaccatgc cggcggcgat ctcctttgcg
     5761 gctcccattt tcgccttcgg cctggtggca ttcggattcc tcggcatggg gccggtgacc
     5821 atcgcagtgg acagcttcgg accggtgtcc gacaacgcgc agtccgtcta tgagttgagc
     5881 cagatcgagt ccatcccggg catcgacaag gacatcgaga agaacttcgg cttcaagccc
     5941 gacttcaagg ccgcgaagca caacctcgag aagaacgacg gcgccggcaa cacgttcaag
     6001 gcgacggcga aacccgtgct gatcggcacg gcggtggtcg gggcgaccac catgttgttc
     6061 gcgatcatcc tcctgctcga caaggaattc ggcggcgtga tcaccaagct ctccctggtg
     6121 cagccgacca tcctgctcgg cttcctcatg ggcggcgcgg tcatctactg gttcactggc
     6181 gcctcgtgcc aggcggtcgt caccggcgcc tatcgcgcgg tggtgtacat caaggagaac
     6241 atggacttga gcgccaagac agcctcgatc gaggacagca aagaagtcgt gaaaatctgc
     6301 acgatctatg cccagaaagg catgttcaat atcttcgtgg ccatcttcag cttgtcgctc
     6361 gccttggcgt ttttcgattc gtatttcttt attgggtacc tgatctcgat cgcgttcttt
     6421 ggtctttacc aagccatttt catggctaac ggcggcgggg cctgggacaa cgccaaaaag
     6481 attgtcgagg tggatttgcg tcagaagggc accgaactcc acgacgcgac ggtggtggga
     6541 gacactgtgg gtgatccttt caaggacacc agttcggtgg cgatgaatcc gatcatcaag
     6601 ttcaccacct tgttcggcct cctggcgacc gagattgcgg tggccatgca gaaggcgcag
     6661 cagactggac tcaagaccgg catcgccgtc gtgttgttcg tcgtggccct ggtgttcgtc
     6721 tatcgttcct tctacggcat gcgcattccg gaagagaagt gacctgcctg gtgaaatcga
     6781 aatcgcgaga cgagcgagcc gccgcacggc ggctcgctcc gccttggcca agacacgggg
     6841 acatttgacc gctatgagga ctgggatttg gtctgaggcg cgccgcgctg gagattgcta
     6901 atggctgcgg ggcgtgcttt gcgtcgggtg tgtgggccag cgggacggaa tattgcgttc
     6961 ctggcgattg cggccgtttt gatcgggtgc ggcacgaggc actaccgcaa atcggccgac
     7021 aaggatgttt atcggatcat cgagcaggtc gaggcccagg ttttcaagcg aaccaacgcc
     7081 ttcaccatcg acacccccta ctcgacgcgg gaccccaaga cgattctgcc ggcggagatc
     7141 gtcgaggatc ggatggccac caatcggcgc gtgatcaatc tgcgggatgc gctggacctg
     7201 gctgtccgca agagccgcga gtatcagaat cagaaagagc agctttacct gcgggccctg
     7261 gcgttgaccg gccagcgcca ccggttcacg cccaacttct tcgcgagcgt caacccctcg
     7321 gcgagcggcg atggcaatgg gaacgtcgat ctggacacgc tgaaaccgga ggtggggttc
     7381 agtcaggtgt tcaagagcgg ggggagcctg agcgtcaggc tcgtcaacga cctcatgcgt
     7441 tactacaccg gggatccagg gacggcggcc ttgagcgtga tgtccgtcga catcgcgcag
     7501 ccgctgctgc gtggctttgg ccggaacagc gatgcggtcg aggcgttgac gcaggcgcag
     7561 cgcagcctga tctacagcat ccgcagcttc gaccagtacc agcggcagtt cgccgttggc
     7621 attgtgaata attacttcgg tctcctgggt caaaaggaca gcgtgcgcaa caactacacc
     7681 aactacctgc gtcgcgtcga aaccaggcgc tacacggagg cgcgcgccgt cgatcgggtg
     7741 cgtcaaagcg aggtggacga tgcgcgcact caggagttga acgcgcggat catgtacgtc
     7801 aactcgttgg ctcagtacat gagccaactc gacgcgttca aggtgaccct gggcctcccg
     7861 ctgaaggagg acctctacct ggacgacggg gacctgcggg agctgagcag cgccgggttg
     7921 ggcgccgtcc cgatcgaccg cgacgcggct tttcgcatcg ccatcgccaa gcacgccgac
     7981 attctcaatg cgatcgacag tttcgaggat gcgaagcgtc gggtcaggct ggcggctgac
     8041 aagctcaaac ccggggttcg tttccaaggc agtgccagct atgccgttca ggattcgacc
     8101 aactcctggt catatcacta cacccggttc aacccggatc agtttcgcta taacttcggg
     8161 ctgcagattg atctccctct ggaccgattg gccgaacgga acaattaccg cggaacgctc
     8221 gtcgatttcg agcgggagtt gcgtcagctc agcctgactc tggacaactt caaggagcgg
     8281 atcgatcggg ggctgcgcac cctcgaacag cggcgcctca attacatcag tcagctcgac
     8341 gcgctcgaag tggcgcggcg gcgggtcgag aatgacacgc tcctgctcga ggctgggcgc
     8401 acggacgttc agaggctgcg ccagtcgcag gacgccctca tccaagccca aaacgacctt
     8461 gtcttcacca tggtggacca cctccaggcc cggttgcaag tcctcttgga cctcggggtc
     8521 atcacaactg accatccgtc cttctggctg caggacccgc tgaccgcggc gctggacgag
     8581 agccagctcg gaccgccgcc cctgcaaatg cccgacgaca acccgatccc cccgaaccaa
     8641 ttcctcgaac cctcgtcatg aatgcatctg cctcgcccga ttccgcggcg ccgccgcgcg
     8701 cacgccgccg gctttttcag gttggcgccg tgggcttgtt gctgctgttg ctctacgttg
     8761 tgctgcgacc gtccaagaag gaaggcgggg gcaccgagca ctaccttgtg aagcgcgggg
     8821 acttcaccgt gtcggtgatc gagccgggca cgttgaccgc cattagcgag gtggtgatcc
     8881 ggagcgaggt cgagggcacg gcccgcatca tctcgattgt gcccgagggt gcctcggtga
     8941 aaaagggcga tctcctggtc gagctggact cctcgcaggc gctggatcag gtcaaccagc
     9001 agctcatcag cgtcgagaag gcgcgcttcg cggtgaccaa cgcggaggcc caactcgaca
     9061 tccagcgcag cgccaccaac gtggattacc aggccgccaa gctcaaactc gacctggcga
     9121 tcatcgaccg cgacaagtac gagcaaggtc agcggttggt cgacctgctc gaagcccaga
     9181 acaaagctct ccaggcgtcg aatcaactcg tggtgaaccg cgagacctac agcaactcgg
     9241 tcatgctcgc caagaagggg tacgagacca aacagaaggt ggatggcgac cggctgtccg
     9301 tgctggcgac cgagaatcaa ctgatcgcgg ctacgaacgt gctttggatg ctcgaccagt
     9361 tcgatgtcga aaagcagatt aagaagttca attcggacgt gcagcaggcg gaagaacagc
     9421 ttcaacagca gatcaacctc aacaaacgga aaatggccca gtacgaggcc gatctgctct
     9481 cccaaatcaa caccctcaag ctcaacgagg acaagctgga acgggatcgc aagaacctcg
     9541 atgcgacgat gatctatgcc cctcaggacg gcctggttgt gtaccaggtc agcgagaatc
     9601 gcttcagcag cgagtcgttg atcgaaggcg gcgcggttgt gcgcaaccgg caggaactca
     9661 tcaaactgcc ggacctttcc cgcatgaagg tcgtggtcaa gattcacgaa tcccacatca
     9721 acatgatccg agccggcctg ccggcctttg tcgtcctgga ttccgatccc gacaagcgct
     9781 atgcgggcgt ggttgcgaaa gtggcgccct tgcccgacac ccaagcgcgt tggggcaatc
     9841 ctaacctgaa ggtttacaac accgaggtgt acctgtccga aaacccgccc aacgtcaaac
     9901 ccggcgtgtc cgccaaagcc gagattgtca tcacgaacat cgccgatgtc ctctcggtgc
     9961 cgatccaggc ggtcacaacc ttcaagggca agcaggtcgt ttacgtgctc aaccgcggca
    10021 agcccgagcc gcgaccggtc gaagtcggca tgttcaacac gaagttcatc cagattgtcc
    10081 agggactcga agccggcgat cgcgtgatgc tctctccgcc ttttgacagc catgagaagg
    10141 atctcgaagg cgccgtgctc gccgacgaag aaaaggccag gatcaccttc accaatgtcc
    10201 cgcccatcga ggctgcgccc cacaacggac tcggcaacca ggccaattct cggggcggag
    10261 ccaacggcag acccgcattg accgcggccg gcaccgaagg cggcggacgt ggtccgacgc
    10321 cgggtgcggg cgcgggatcc ggacctcttg gaggcgtcat ccccgaagaa atcctcaaac
    10381 agtacgacaa gaatggcgat ggaacactcg acgaaacgga acgcgagtcg atgcgcgcgg
    10441 cgttcgcgtc ccgcttcggc ggccagggct cgccagccgg aggaccgggc ggagggatgc
    10501 gtccgggccg tgaggagatg atgaagcgtt tcgacaagga tggagacggc cagctcgatg
    10561 aagaggagcg ggcggccatg cgcgaggcga tgggcagtct ccgacccaat cgcggcgaag
    10621 gggaagccgg cgcccgccct ggtcgagacg cagctcccag tccgcgacag ccctgagtca
    10681 tgccagccga ttcggccatc atcaggattc gcgacgtcga gaagcgttac gttctcgggg
    10741 gcgaagccga tgtcctggcc ctgcgcggtg tgaatctcga tgtcgaagcc ggatcctatc
    10801 tggcaatcat ggggccgtcc ggatcgggca agtcgacgat gctgaatata ctcggttgcc
    10861 tcgatcgccc gaccgctgga cggtatttcc tgggtggcga ggatgtttct cgaatgcccg
    10921 atgaccagct ctcggaagcg cgcggactca agatcggttt catttttcag tcgtacaacc
    10981 tgatcgcgca gctcacggtt ctcgagaaca tccaggtgcc gttgctctac caggggaagg
    11041 atgtccgcac ccacctcgaa tcctgcctcg aactggccaa gctcgtcggg ttgggcgacc
    11101 ggctccatca tcgccccagc caattgtccg gtggccagca gcagcgggtt gccgtggccc
    11161 gctcgctggt caacaaccct ctcatgatac tcgcggatga accgacgggg aacctcgact
    11221 cgaagaccgg ccaggaagtg ctcgacctca tcgaccgcct gaacgaggcc ggcaagacca
    11281 tcgtcctcgt cacgcacgac gaccgggtgg ccagccgggc cgaccgggtc atccatatga
    11341 aggatggcct gatcgaccgc gaagttgtca accgccccat gcgggcccgc ccggccgttt
    11401 cgtcgtccta accccccggg gcccgcatgc tcaccttcca attcgtaagc accgtgcgcc
    11461 tgggcatcaa gagcctgatg ctccacaagc tgcgttcggc cctgacgatg ctcggcatca
    11521 ttttcggcgt ttgctctgtc atcgccatgc tcgcgatcgg ggagggggcc tcgtacgaag
    11581 cccaggaagc catcaagaat ctcggcagca acaacatcct gttgcgcagt ctcaagccgc
    11641 ccgaccaggc ccgccagcag gcaggcggca gcccgcgcgg catgcagttg gcctatggcc
    11701 tcacctatga agatggcgcc agaatccagt ccaccatccc gggcgtcaac cgcgttctgc
    11761 ccatgcgcat catccgcgag aatgtgcgct tcgatcgcaa cgaaatcccc tgccaggtga
    11821 tcggcacgct gccgtcctac atggctgtga ctggggcggg gatcgtccgc ggacggttcc
    11881 tcgcgcatct cgacgaacag cacgcggaga atgtttgcgt gatcacgacc ggcctggcgc
    11941 gccgtctctt cccgcacctc gatccgatgc agcacgcggt gaagatcgac accttctact
    12001 accgaatcat cggcatcctg caagagcgca gccaacccga gcagcgcacc caggctggcg
    12061 ccatggaggg acaaccgctc gacaacaacg tctatattcc gctgagcgtt tcccgaacgc
    12121 gattcggcga gacgctcatc cgcagatccg ccggcagttt cgaggccgag gaggttcaac
    12181 tccaccagat caccgtccag atgcagaatc tggcgtccgt ggaaaccgcc gacccccaaa
    12241 tccgaaccct cctcggtcga ttccacgacc agaacgatta cgagatcatc gtgcctctcc
    12301 aactcctccg ccaggccgag cagaccaagc gcatcttcaa catcgtgctc gggtcgatcg
    12361 cggcgatttc actgctcgtc ggcggtatcg gcatcatgaa catcatgctc gcgaccgtga
    12421 ccgaacgcac ccgcgagatc ggcatccgcc gcgccctcgg cgccaagcgc cgcgatatca
    12481 ccacccaatt cctggtcgag actgtcgtgc tttcggtcgg cggcggattg gtcggcgtgc
    12541 tcgtcggcgt cctcacgccc ttggttgtct cgcagctcac aaccatgaag acgatcgtca
    12601 ctgtctggtc ggtcctgctc gcgtttggaa tctccggcgc ggtaggcatc gttttcggtc
    12661 tctatcccgc gagcgccgcc gcaaagctcg atcccatcga ggcgctgcgc cacgagtgac
    12721 gaggatcgac tgaatcccaa acagcacaaa taaatccaat gaaagctaag agatggccca
    12781 gagctaagtg ctcctgcata gcgcgcctcg gaacaggtaa agaattctgc tattgtcgct
    12841 gccatgcatc tttccctaaa aatgcggaac caacatgtgt tcccagacaa ccttgactta
    12901 ttgtgcgcag cgatcgaatc aaccactttt actgaacctc acgcagaggt gctctgcgtc
    12961 ccctccaggc gcagcgttga gcgcgcgtcg cgccgactcc ccacctcgaa tccatagacc
    13021 tccgcgttcg ccggaggtca tctcatggct cattttggct ctggcgttgg tctgcgtcgc
    13081 aggtcacgcc atgccctctt atgacgacgt gctggtcgtc gtgaaacagg gcgacgccga
    13141 gtcggccgcc gccgcccagt acttcaagga gcagcgtcga ataccggacg aaaacctggt
    13201 cactctgcct ttgacgggtg ccattcccac cgtcgcggct cgggacgcct tcatcggcgg
    13261 aatcaaggcc catatggcgc agaacggtct gcagggccgc atcaactaca tcgtcctgag
    13321 ttcgaacttc ccgcgcgagt gcaaggacac ggcgacggcg ccgtacaacg cgatcttcga
    13381 tgcctacctg atgtgggccc tgtccgacac ctatccatcc acgagttact tggattgcat
    13441 ccctctgcac aacaacccct attattacat ccgcaagtcg aactacgaag atcgcacggc
    13501 gataaagttc acgaaaacta agtatcgcta ttacattgtc tcacggctcg atggagaggg
    13561 gttggccgcc acaaaacagt tgatcgatca ttccggacat gttgcttacg accaatacaa
    13621 gaaggggatc aatttcctgg tcgacgacga tccctcgaat tgggaggtct acagatatca
    13681 agccgagcgc gcggaactga aggaacgcgg catcaacatt atcgattgga atcccggcgt
    13741 ctcgggctcg attcacaacc tgcacggtct gagtttcgcc tacttcaact gggtcaacgg
    13801 cacctcggac cctttcatca gctaccgcaa catttcgaaa cacatgcgat tcaacccggg
    13861 tgccctggtg ttcgcctacc gttcgtatcc ggcccgtctc ttcaagcgac cgctctgcgg
    13921 gttgagtcaa tgcaacgtca acacccgtgc ggtgcagagt ttgatgaaga gcgacgcctc
    13981 ggacctggtg ttgcggcaga tgtcggatgt ggcctacgcg accgaaaaca actcggtgtg
    14041 gtgcgccctg ggccaggaca tcctgaagat gtacgcgacg gccaacgcgc gcagcgccaa
    14101 caattttcgg ggcaacggag tggcggtcta cgcgcccgac ggcaccctgc tcaatcactt
    14161 cacga
//
LOCUS       DAOQRO010000037        14113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3372_length_14113_cov_5.120856, whole genome shotgun sequence.
ACCESSION   DAOQRO010000037 DAOQRO010000000
VERSION     DAOQRO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14113
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3372_length_14113_cov_5.120856"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKK20_02570"
     CDS             complement(<1..450)
                     /locus_tag="PKK20_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98802.1"
                     /translation="MRLLLLLMLLLMLSPLVVRTAIGSTAASELPLPPRAAVWQGEAT
                     SENRGFAREIGRLVQSAGWDIEFLDSAALTNVTQLTPDRYGLLVLPGARRLPMESIAS
                     IQSYLGAGGDMIALGLPAWDTPVFSLGDHWMTRDEYEVALADQKPQRY"
     gene            619..2862
                     /locus_tag="PKK20_02575"
     CDS             619..2862
                     /locus_tag="PKK20_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98803.1"
                     /translation="MKETDNVMMSLRRSGQRRRALAWLTLAAGLVLSGALARAELQFD
                     VFVGYGSGGGNDGIVREAAWFPVACEIYNDGPAFDAVFELSSRQVGAGQARRMRIELP
                     TNTRKRFSIPVFAGASRYASWDARLYDAKRKLRGERLDLRAKDIGWETFIVGGLARTF
                     GGLPTLPEVRGGRPETQPQVARMTPEQFPDQSLALEGLGTLYLNSEKALELKVNQVNA
                     LLAWLHGGGHLVVAPEQAQDIESLKWLRGLLPVTLGEIRTNRSQGVLHEWLRSGADLR
                     SSRSFNPVPGASRTPGRPPPAASGADPYAALQPDPAFELADFPAFSTVMNDGEAVVSL
                     GGGPLIVTAPRGRGQVTVLAFSPEREPFKSWKNRSWFWARLFKLPGDTLNEQTQFYYG
                     GWSVDSVLGAMIDSRQVRKLPVEWLLALLVVYLLVIGPIDHWWLKKINRQMLTWITFP
                     IYVVAFSLLIYFIGYKLRAGETEWNELHLVDVLPRAGDVELRGRTYASLYSSVNARYR
                     LAGDQPFAALRGEFLGPSGGGQESSRVEAEIVANSFRAEVEVPVWTSLLYVSDWTEPA
                     AAPLALDIQEEGSSIRVTVRNGLSHAFSSLHLVLRGQIYELGGLDAGETKAAQVQLST
                     GQALELFVRRFGQGFTGAASARRQAFGSTAQARLEANPNTLIAATFIGQLGLYQGQQR
                     AFVYPPGFELSSLVARGDAVLLAWDAGGSPTAGSMLKHKPPRRAQSTLYRLAVPVSGR
                     APAAPVL"
     gene            2871..3857
                     /locus_tag="PKK20_02580"
     CDS             2871..3857
                     /locus_tag="PKK20_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98804.1"
                     /translation="MQATPVSNASQAQAGTDPKPAVRTLNLTRLYGDMVALDNLSLTV
                     NQADLFGFIGSNGAGKTTTLRILATFLMPSAGTVEVLGHDVVKDADAVRHIIGYMPDF
                     FGVYKDMEVTEYLDFFGACYKIPAHQREKTVNDVLELVGLTEKKGAIIGALSRGMQQR
                     LGLARVLIHDPRVLLLDEPASGLDPRARIEMMAILQELQRLGKTIIISSHILSELETL
                     CNRVAIIEKGKLIYTGPVQGVRDQMASGRVVWVRVAGETDRAVALLKAQPEVSEVELT
                     DGRLKVTLAEPDADHSIIATALVQGGARLLELREDELGLEDVFLRVTKGETQ"
     gene            complement(4083..5330)
                     /locus_tag="PKK20_02585"
     CDS             complement(4083..5330)
                     /locus_tag="PKK20_02585"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNU98805.1"
                     /translation="MSDSYIQNLFAERIGGRRYGKSTAIYKFEKIKRARRAALEANPG
                     SEIIDMGVGEPDAMAFPEVVEELAREARKPENRGYADNGDAVLKRAAARYLDRVCGVP
                     GIDPDTEVLHSIGSKAALSILPAALVNPGDCVLMTTPGYPVFGTHAAYYGGRVHNLPL
                     TEANGFLPDLESVPDAVLRRTKALVLNYPNNPTGASATPEFFREVVAFARRRGIVVVH
                     DAAYAALVFEGQPLSFLATPGAKEVGVELHSCSKSFNMTGWRCGFVAGNPLLVKAYGD
                     VKDNTDSGQFLAIQHAAAYGLDHPEITRQIAAKYSRRMTALVETLRAAGFGARKPKGS
                     FFLYVKAPRAATPHQGPRIEFASAEEVSQWLITERLISTVPWDDAGAYLRFSVTFAAR
                     DAAEEQRVLAELGRRLADVRFEF"
     gene            5843..9403
                     /locus_tag="PKK20_02590"
     CDS             5843..9403
                     /locus_tag="PKK20_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNU98806.1"
                     /translation="MVLALALVLGSSQSVQPAERDDRFFDAAPFARVCTWDSRRDVGV
                     RANRLEEIAAEDLFLATDLMLGAEGEYRVESAANGEACIGLEWIEARPLTRLQIRLKT
                     PPARPVRIQFWRGETVWQGQWASIDGAMEGEGLVWTFTPAKVGGLYWKVRWIVFAGGH
                     PVAVTGFEAITSSTLTASDLLIQSDPDRPGQMAGLEVYNGSIVGIEGTRLSWDMAAPL
                     QLGVRHVACEALRQVRADRTIIRFRLPEGAFAVAVDDVLERGPVYLPHAGLFVSRFPA
                     APSLGEYKRQIADKQTVLERVRAMPDQTRKRAMKVTHCDPRLDAGRMMLSLACDNAKF
                     VTEANGTVFYFENFDSDEKGHRPSYTLTPVVGNRRVLGQSQAWGLLGLNKAAHADPAA
                     ALALRINETDFARGLGHHASGDLTIDVSEGYARFEAEVGLQWQGGNAPGTVVFQVLVD
                     GEKRFDSGVMREREDPKPISVPLAGARVLTLRLTDAGDGIGFDAGNWCDARLIRADSA
                     EPVYLTELFTPSPRFKRHLEGGWLPAPVTTVNIGGIEYRQRTYVTPLETENLSTAPRW
                     LNAKPLAVLELTMTNHNPEPADASASFVLAMGPGGGGLPVCKTTGQRTIIVADGRLVG
                     VFDSAGFPAGMGRSDNGHLSISGLLPANGSARCCFYLPAWEATLEEQAELGGGEALFA
                     SFKAYWEAIMAPAMQIELPDAWLADVIRANQAHVLLAARNERQGTQIEPWIASDRYLV
                     AIDSEGNSPIRGMQYWGHFDYAQRAFEYSFNHYKPEGFMTMGYTVMGSGWHLWALGEY
                     MRLARDDAWFRAVADKPAGMCRWVTAQLAKTRRLKPDGRPVPEYGLMPPGVQADWNAY
                     AYYFYANSYFHAGLGAMGHALGLIGHPDADAVLAAAVQLGKDVRRAFGQAQALAPVVP
                     LQNGTWVPYYPASVYTPGPMADFYPGQDGNRSWAYDVDLGPHHMVPLGTMPPEAPEVD
                     WIMDHMEDVQFLESGWGGYPAERNRADWFDMGGFAKVQPYYARNAEVCALRDDVKPFV
                     RSYFNTLASLIDGTSLSIYEHFSNWCYNKTHETGYFLHQSRTMLLTERGDELWLAPLL
                     TDNWLEDGMKIRVANAPTFFGPVSYQIVSSVDCGHIDALIDPPTRSAPKAIVLRLRHP
                     EGRPIKSVQLDTGARATFDNTLQIVRMAPSAGPIRVRVEY"
     gene            9427..9582
                     /locus_tag="PKK20_02595"
     CDS             9427..9582
                     /locus_tag="PKK20_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98807.1"
                     /translation="MIESACRLLAALGMGYLSQIALDCTTPATIDLPWLIGSKTGWPR
                     LLACIRA"
     gene            complement(9876..11255)
                     /gene="mgtE"
                     /locus_tag="PKK20_02600"
     CDS             complement(9876..11255)
                     /gene="mgtE"
                     /locus_tag="PKK20_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNU98808.1"
                     /translation="MIGRLLKPELTGLIQEREFNQLRQILAEFPPQDIADILTDLAPE
                     DRAVLLRILPYQLAADVFEQLGVEEQERLVLALGNEQVATILNEMEPDDRTALLEELP
                     AAATQKLLNLLSPEERRIAVTLLGYPEESVGRRMTPEYVAVKHDWTVAEALEHLRRVG
                     RHRESLNQLFVVDPQGRLAGVVRLRNLVVAELDTPVANLLEPQVISLRATDDQETAVA
                     EFQKYDRTMLPVVDSQEVLVGVVTVDDVLDLVEEEVTEDMQKMGGMQALDASYLGTDL
                     LVLIRKRVGWLILLFLGQMLTATAMGHYEGDLANALVLALFIPLIISSGGNSGAQAST
                     LVIRAMATGDVHLQDWLRILRRELALGLALGAVLAVIGFLRIALWPNRASLYTDHYAL
                     VATVVGLSLVGVVAFGSLVGAMLPLALRRLRLDPATCSAPFVATLVDVSGLIIYFSIA
                     QAVLRGTLL"
     gene            complement(11433..12524)
                     /locus_tag="PKK20_02605"
     CDS             complement(11433..12524)
                     /locus_tag="PKK20_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98809.1"
                     /translation="MTTPVRVAIIGCGSITLQNHLPGLALCPDTQVTALCDANPAVLE
                     RARGQTGVAALSTRFEEVVRRDDVDAVIIATPNHTHPRIALAAVAHGKHLLCEKPLAL
                     NAADAFAMAEAAERAGVRHMTAFTYRFVPAMRYLHDLVKRGDLGQPYHYRSCRLQDWG
                     TRNLGWRQVRALAGTGELGDMLSHRIDFAHLLIGPMRRLVAQLKRIHPVRGDAPSDLD
                     DWVALLAEFECGATGVLESSKLASGRNESWRSLDSVEVNGSERSFVFITGRWNQIETG
                     RAGGPGLETIPVPEEYWRWPGSPRDPRVGDPLVTFRYDQAWEFVDAIRQQRPCVPDFF
                     DGARVQAVMDAAVRSAETGQWMGLAPNPS"
     gene            complement(12734..>14113)
                     /locus_tag="PKK20_02610"
     CDS             complement(12734..>14113)
                     /locus_tag="PKK20_02610"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNU98810.1"
                     /translation="DILIRDGKVAALGEGAAAQAPPDADRFDATGRIVCPGLIDLHVH
                     LREPGQSSKETIATGTAAAAKGGFTSVVCMPNTVPPIDSPSTVALVHERVASEGRVNV
                     YVAGAISKGLAGEELGPMGSLKRAGVVAVTDDGHCIQNNELMRHALEYSTMFDLLVMD
                     HCQDYSIVTDGVAHEGYYSAMLGLRGWPAAGEELIVARNILLAERTGARVHCQHISSA
                     GSVRLLRKAREQGLPVSGEACPHHFTLTDAALAGSDEFWRKDGQAVLISGKSSSALPF
                     WPAYDTHVKMNPPIRAAADREALLEALADGTIEVISSDHAPHCNYEKDVEFDDAPFGI
                     TGLETELALSLMQLYHAKRLGLSELIARFTTGPARLLKLTGKASLKPGSDGDVTVFDP
                     DVEWEFHREDTAGKSCNSPFYGWPMKGKALATIVAGRIVWRDPDRAASESIQQQNQTN
                     PTSRKNKKI"
BASE COUNT         2385 a         4317 c         4818 g         2593 t
ORIGIN      
        1 gtaccgctgg ggcttctgat cggcgagtgc gacctcgtat tcgtcccgag tcatccaatg
       61 gtcgcccagg ctgaacacgg gggtgtccca ggccggcagg ccgagggcga tcatgtcgcc
      121 gccggccccg aggtaggatt gaatcgaggc gatgctctcc attggcaggc ggcgggcgcc
      181 gggcagaacc aggagtccgt agcgatccgg cgtcaactgc gtgacgttgg tcagggcagc
      241 gctgtccaag aattcgatgt cccagcctgc actttggacc aggcgcccga tctcacgggc
      301 aaaccctctg ttttcggaag tcgcttcgcc ttgccagacg gcggcgcgcg ggggtaacgg
      361 gagttcggag gccgcggtcg atccgatcgc cgttcgaacc accagcggcg acagcatgag
      421 cagcagcata agcagcagga gcaggcgcat gcgtggattc tagccccttg ccggaagaga
      481 tccaacatgt tgtgggtccc gatcgagccc cgcgcggcgg ggacgcatct ttccgcgacg
      541 catgagcgga aagatggaat tcgggcgttg cgtccggggc ggggtggact ataacgctgg
      601 tgactggatt tgggcgacgt gaaggaaacc gacaacgtca tgatgagctt gcggcggagc
      661 ggacagcgtc gccgggcgtt ggcttggttg acactggccg cggggttggt tttgtccgga
      721 gcgcttgcgc gcgcggaact gcagttcgat gtgtttgttg gctacgggag tggcggtggc
      781 aacgacggga tcgtccggga ggcggcgtgg tttccggttg cgtgcgaaat ctacaacgac
      841 ggtccggctt tcgatgcggt gttcgagctt tcgtcgcggc aggtcggggc cggccaggcg
      901 cgacgcatgc ggatcgagtt gccgacaaac acgcggaagc ggttttccat cccggtgttt
      961 gcgggggcga gccggtatgc gagttgggat gcgcgcctct acgatgccaa gcgcaagctg
     1021 cgcggggaac ggctcgattt gcgggccaag gatatcggat gggagacgtt catagtgggc
     1081 ggattggccc gcacgtttgg cgggctgccg acgctgcccg aggtgcgcgg ggggcggccg
     1141 gagacccagc cgcaggtggc gcggatgacg cccgagcagt ttcccgatca gtcgctggcg
     1201 ctcgagggac tgggcacgct ctacctcaac tccgagaagg cgctggaact gaaggtgaac
     1261 caggtcaatg ccctgctcgc gtggttgcat ggcggcgggc acctcgtcgt cgctccggag
     1321 caagcccagg acatcgaaag cctgaagtgg ttgcgcgggt tgctgccggt gaccctcggc
     1381 gaaatccgca ccaaccgcag ccagggcgtc ctccacgaat ggctccggtc cggcgccgac
     1441 ctgcgctcct cgaggtcctt caacccggtg cccggtgcga gtcgcacgcc gggccgtcca
     1501 ccgccggcgg cgtccggggc cgacccgtat gcggccttgc agccggaccc ggctttcgag
     1561 ttggcggact ttccagcctt ctcgaccgtc atgaacgacg gggaggcagt ggtgtccctg
     1621 ggcggcgggc cgctgatcgt gaccgccccc cgcgggcgcg gccaggtgac cgtgctggcc
     1681 ttcagtccgg aacgcgagcc gttcaagtcg tggaagaacc gatcctggtt ttgggcgcgg
     1741 ctcttcaagc tgcctggcga cacgttgaac gagcagacgc agttttacta tggcggctgg
     1801 agtgtggaca gcgtgttggg cgcgatgatc gattcgcggc aggttcgaaa gctgccggtc
     1861 gagtggctgc tggcgctgct tgtggtgtat ttgcttgtga tcggcccgat cgatcactgg
     1921 tggctcaaga agatcaatcg ccagatgctg acctggatca ccttcccgat ctacgtggtg
     1981 gcgttttcgc tgctgattta tttcatcggc tacaagctgc gcgcgggtga gacggagtgg
     2041 aacgaactgc atctggtcga tgtcctgccg cgcgccggcg atgtcgagct gcggggccgc
     2101 acctatgcat cgctctactc gtcggtgaac gcccgctaca ggctggcggg agatcaaccg
     2161 ttcgcggcgt tgcgcggcga gtttctcggg ccctcgggcg gggggcagga gagcagccgg
     2221 gtcgaggccg agattgttgc gaacagcttt cgagcggagg tcgaggtgcc ggtctggacg
     2281 tcgctcttgt atgtcagcga ctggaccgag ccggctgcgg cgccgttggc tctggacatc
     2341 caggaagagg gcagctcgat ccgcgtgacg gtgcgcaacg gtctgtccca tgcgttttcg
     2401 agcctgcacc tggtgttgcg ggggcagatc tacgagttgg gcggcctcga tgccggagag
     2461 accaaggcgg cgcaggtcca gctctccacc ggtcaggcgc tcgaactgtt tgtgcgtcgg
     2521 ttcggacagg ggtttacggg ggcggcgtcg gcgcggcgcc aggcctttgg gagcacggcg
     2581 caggcgcggc tcgaagcgaa tccgaacacg ctgatcgccg cgacgttcat cggccagttg
     2641 ggtttgtacc agggacagca gcgcgcgttt gtttatccgc cgggattcga gttgagttca
     2701 ttggtcgcgc gcggcgatgc cgtgctcctg gcgtgggacg ccggcggcag cccgacagcc
     2761 ggttccatgt tgaagcacaa accgccgcgc cgcgcgcaga gcactctcta ccgcctggcc
     2821 gttcccgtga gcgggcgggc gccggcggcg cccgtccttt gaaagacccc atgcaagcga
     2881 caccggtaag caacgcctcg caagcccagg ccggcaccga cccgaagccc gccgtccgaa
     2941 cgctcaatct gacccgtctc tatggggaca tggtggcgct cgacaatctc agcctgacgg
     3001 tgaatcaggc cgatctgttc ggcttcatcg ggtcgaacgg ggcgggcaag accacgaccc
     3061 tccggattct ggcgacgttc ctgatgcctt cggccggcac ggtcgaggtg cttggacacg
     3121 atgtcgtcaa ggacgccgat gcggtgcggc acatcattgg ctacatgccc gacttcttcg
     3181 gggtgtacaa ggacatggag gtgaccgagt acctcgattt cttcggggcc tgttacaaga
     3241 tacccgcgca tcagcgcgag aagacggtga atgacgtgct cgagttggtg gggttgaccg
     3301 agaaaaaggg ggcgatcatc ggggcgctga gccgcggcat gcagcagcgg ctcggtttgg
     3361 cccgcgtcct gattcacgat ccgcgggtgt tgttgctgga tgaaccggcc agcggcctcg
     3421 atccgcgcgc gcgcatcgag atgatggcca tccttcagga actccagcgt ctgggcaaga
     3481 caatcatcat ctcgtcgcac atcctcagcg aactcgaaac tctctgcaat cgggtagcca
     3541 tcatcgagaa aggcaagctc atctacaccg gccccgtcca gggagtgcga gaccagatgg
     3601 ccagcggacg cgtggtctgg gtgcgcgtgg cgggcgagac cgatcgggcg gtcgcactgc
     3661 tcaaggcgca gcctgaggtg tcggaggtcg agctgaccga tggccggctc aaggtcaccc
     3721 tggccgagcc ggacgccgac cacagcatca tcgccacggc gctcgtccag ggcggcgcca
     3781 ggctgctcga attgcgcgag gatgaactcg gcctcgaaga cgtgttcctg cgcgtgacca
     3841 agggcgagac tcagtagaag gccgagggtg ttggccatga acctcgacat ggaaagcggc
     3901 gccgaagcgc gcgcaccaag cgctcgcgcg gcgtcgaggg cgcccctcgt ggcgaagccc
     3961 ctggagtgcc tgcctgccgc gctgcgcttg ctcaggcagg cggcggttta ccgccgactc
     4021 gggttcatct tcatcgagca gttccgcaag gagcgggtgg ccctcgagga aggcccgcac
     4081 cctcagaatt caaaccgcac gtctgccaat cgccgaccga gttcggccag cacgcgttgt
     4141 tcctccgcgg cgtcgcgcgc ggcgaaggtg acgctgaagc gcagatacgc tccggcatcg
     4201 tcccacggca ccgtcgaaat cagacgctcg gtgatcagcc actgcgacac ctcctcggcc
     4261 gacgcgaact cgatgcgcgg tccttgatgc ggagtggcgg cccgcggggc cttgacgtag
     4321 aggaagaatg accccttcgg cttgcgcgcg ccgaagcccg ccgcccgcag cgtctcgacg
     4381 agcgcggtca tgcgccgcga atacttcgcc gcaatctggc gcgtgatctc cggatgatcg
     4441 agtccgtacg ccgcggcgtg ctggatggcg aggaactggc ccgaatccgt gttgtccttg
     4501 acgtcgccgt aggcctttac gagcagggga ttgccggcga caaatccgca gcgccatccg
     4561 gtcatgttga agctcttgct gcacgaatgg agttcgactc cgacctcctt ggcgccgggc
     4621 gtggccagga aactgagcgg ctggccttcg aacacaagcg cggcataggc ggcgtcatgg
     4681 accaccacga tcccgcggcg tcgggcaaaa gcgaccacct cgcggaagaa ctcgggtgtg
     4741 gcactggcgc cggtcgggtt gttggggtag ttcaggacca gggccttggt ccggcgcaag
     4801 acagcatccg gaacggactc gagatcgggc aggaagccgt tggcttcggt caatggcagg
     4861 ttgtggacgc gtccgccgta gtaggcggcg tgggtgccga acaccggata gcctggcgtg
     4921 gtcatcagga cgcaatcgcc gggattcacg agcgcggcgg ggaggatcga caaggcggcc
     4981 ttgctgccga tcgagtgcag gacctccgtg tccggatcga tccccggcac gccgcacacg
     5041 cgatcgagat agcgcgccgc cgcccgtttc agcaccgcgt cgccgttgtc cgcatacccg
     5101 cggttctccg gcttgcgcgc ctcgcgcgcg agttcctcga cgacttcggg aaaggccatc
     5161 gcgtccggct cgcccacacc catgtcgatg atctcggagc ccggattggc ttcgagggcg
     5221 gcgcgtctgg cgcgcttgat cttctcgaac ttgtagatcg cggtcgactt gccgtatcga
     5281 cgcccgccaa tccgctccgc aaagaggttt tggatatagg aatcgctcat aatcctgctg
     5341 cgcgaaccgc gccaatgcgc cgccgccccc gtatgaaaac cgggcgcgca aactaatcgc
     5401 catcaagctc cttggcaagc gtgaggcgcc ggtttcctca ggtttgcgat tcaagcctcg
     5461 gtcaactcca ctcattggcg acggagtcga aataggtgtc ggaatacgca gaattggaag
     5521 cgccggttgc gcaggcgttc ccggtcgagg ttcacggtcg tggtggtttg gtcggcggcg
     5581 agtgtgatca cctgcatcgc caacagcgcg caaagggtga aaagagggat tgcaacctgt
     5641 gtggacagca gaccgatcct gcgaatatcc tggagcgatg tttcatatag cttcgcggag
     5701 tctcagatcg gggccgatca tgatctctgt gttcttcagg gcgaggcgcg cgtcagacca
     5761 gtctcgacgc tcagcttcaa gaacaacccc aatgccttgt cggctctctg ttcggggcgc
     5821 cctcgggcgc ggttctttca gcatggttct ggcgttggcg ttggtgttgg ggtcgagcca
     5881 gtcggttcag ccggccgaac gcgacgatcg cttcttcgac gccgctccct ttgcccgggt
     5941 gtgcacctgg gattcgcggc gtgacgtcgg cgtccgggcc aatcgtctcg aggagattgc
     6001 cgctgaggac ctgttccttg cgacggatct gatgctcggc gcggagggtg agtacagagt
     6061 cgagtcggcg gccaacggcg aggcctgcat cggactcgaa tggatcgagg ccagaccgct
     6121 cacgcggctg caaatccgat tgaagacgcc gccggcgcgt ccggtgagga ttcaattctg
     6181 gcggggcgag accgtctggc agggccaatg ggcatccatc gacggtgcaa tggaaggcga
     6241 gggcctcgtc tggacattca cgccggcgaa ggtcggcggg ctgtactgga aagtccgctg
     6301 gatcgttttt gccggcggtc acccggtcgc agtcacgggt ttcgaggcga tcaccagctc
     6361 gactctgacg gcttcggacc tcttgataca aagcgatcct gacaggccgg ggcagatggc
     6421 cgggctcgag gtctacaacg gctcgatcgt cgggatcgag ggcacgcggc tatcctggga
     6481 catggcagcg ccgctgcagt tgggcgtgcg tcatgttgcg tgcgaggcgc ttcgtcaggt
     6541 ccgtgccgat cggaccataa tccgttttcg cctgcctgag ggggcttttg ccgtggccgt
     6601 ggatgacgtc ctcgagcgcg gtccagtcta cctgccgcac gcgggtttgt ttgtcagccg
     6661 gttcccggca gcgcccagcc ttggcgagta caagcgccag attgcggaca agcagactgt
     6721 cctcgagcgg gtgcgggcga tgccggacca gacccgcaag cgggccatga aggtcacgca
     6781 ctgcgatccg cgactcgatg cggggcggat gatgctttcg ctggcgtgtg acaacgccaa
     6841 gttcgtgacc gaggcgaacg ggacggtttt ttatttcgag aacttcgaca gcgacgagaa
     6901 ggggcatcgt cccagctaca ccctgacgcc ggttgtaggg aaccggcgcg tgctgggtca
     6961 atctcaggcg tggggcttgc tgggattgaa caaggcggcg catgccgatc cggcggccgc
     7021 tttggccctg cggatcaatg agacggactt tgccaggggt cttgggcatc acgcctcggg
     7081 cgacctcacg atcgatgtca gcgaaggcta tgcccggttc gaggccgagg tcggtctgca
     7141 atggcaaggg ggcaacgccc cgggcaccgt ggtctttcag gtgttggtgg acggcgagaa
     7201 gcggttcgac agcggcgtga tgcgcgagag ggaggacccc aagccgatca gcgtgccgtt
     7261 ggccggggcc cgtgtgctca cgctgcgatt gacggatgca ggggacggca tcgggttcga
     7321 cgcgggcaac tggtgcgatg cgcgtctgat ccgcgcggac tctgctgagc cggtctatct
     7381 caccgagttg ttcaccccga gtccgcggtt caagcggcat ctcgagggcg gatggcttcc
     7441 cgcccccgtg acgactgtaa acatcggagg catcgagtat cgccagcgga catatgtcac
     7501 gccgctcgag acagagaacc tctcgaccgc tccgcgctgg ctcaatgcca aaccgcttgc
     7561 cgtcctcgag ttgaccatga cgaaccacaa ccccgagcca gccgacgcaa gcgcgtcatt
     7621 tgtgcttgcg atgggtcctg gcggtggcgg gctgccggtc tgcaaaacga ctggccagcg
     7681 gacaatcatt gtcgccgatg gccggctcgt gggggttttc gatagcgctg gatttccggc
     7741 cggcatgggg cgatcggaca acggccatct ctcgatcagc ggtctgttgc cggcgaacgg
     7801 cagcgctcgc tgctgtttct acctgccggc ttgggaggcg acacttgaag agcaggccga
     7861 gcttggtggc ggcgaggcat tgtttgccag cttcaaggcc tactgggagg cgatcatggc
     7921 cccggccatg cagatcgaat tgcctgacgc ctggctggcc gacgttatcc gcgcgaacca
     7981 ggcccatgtg ctgctggccg cccgcaacga acggcagggc acacagatcg agccgtggat
     8041 tgcgtcggat cgctacctgg tggcgatcga cagcgaaggc aactccccca tccgcggcat
     8101 gcagtactgg ggccacttcg actatgcgca acgggccttc gagtactcgt tcaaccacta
     8161 caaacccgag ggattcatga ccatgggcta taccgtcatg ggcagcggtt ggcatctgtg
     8221 ggccttgggc gaatacatgc ggcttgccag agacgacgcc tggttccggg cggtggccga
     8281 caagccggct gggatgtgcc gctgggtcac ggctcagctt gccaagacgc gcaggctcaa
     8341 gcccgatggg cgaccggtgc cggagtatgg cttgatgccg cccggtgtcc aagcggattg
     8401 gaatgcgtat gcctattact tctacgcaaa cagctacttc catgctggtc tcggagcgat
     8461 gggccacgcc ctcgggttga tcgggcatcc tgacgccgat gccgtcctgg ccgccgccgt
     8521 gcaattgggc aaggatgtgc ggcgggcgtt cgggcaggcg caggctctgg cccctgtcgt
     8581 gccgctgcag aacgggactt gggtgccgta ctacccggcc tcggtttaca cacccggacc
     8641 gatggctgat ttctaccccg ggcaggatgg caaccgcagt tgggcctacg acgtcgatct
     8701 cgggccacat cacatggtcc cgttgggcac catgccgccc gaagcgcctg aggtcgactg
     8761 gatcatggac cacatggagg acgtgcaatt cctcgagagc ggctggggcg ggtatccggc
     8821 cgagcggaac cgtgccgact ggttcgacat gggcggtttt gccaaggtgc agccgtacta
     8881 tgcgcgcaac gccgaggtct gcgcgttgcg cgacgacgtc aagccgtttg tgcgcagcta
     8941 tttcaacacg ctggcatcgc tcatcgacgg gaccagcctg tcgatctacg agcatttctc
     9001 gaattggtgc tacaacaaga cgcacgagac gggctacttc ctacaccaat cgcggacgat
     9061 gctcctgacc gaacggggcg atgaactctg gctggcgccc ctgctgaccg acaactggct
     9121 cgaggacggc atgaaaatcc gcgtcgcgaa cgccccgact ttcttcgggc cggtgagtta
     9181 ccagatcgtc tcgtcagtcg actgcgggca tatcgatgcc ctgatcgatc cgccgacgcg
     9241 gtccgcgccg aaagccatcg tgctgcgatt gcgtcatccg gaaggccgac cgatcaaatc
     9301 cgtgcaactc gataccgggg cccgggcgac attcgacaac acactccaga ttgtccgcat
     9361 ggcgccatcc gccgggccca ttcgggtgcg ggtggagtac tgagccggag gaatcgggac
     9421 gccgatttga ttgagtcggc ctgtcggctt cttgccgcgc tgggcatggg gtatctcagc
     9481 caaatcgccc tcgattgcac gactcccgct accatcgatc tcccctggct gatcgggagc
     9541 aaaaccggtt ggccgaggct gctggcctgc attcgagcat gagggcgtgt tcgaggagag
     9601 catgctgcgc gccaccgacc acgtcctgcg ccagcgtcag attctcgagt atcgcccagc
     9661 cagttggcgt ttctgcgctc gcgacaggcg gatcgacctc ctgcttgacc acgggcttgc
     9721 gttggccggg gacatcgtgc gccgcgcgat cggtttcccg cgagcggcgg atgtcggtga
     9781 accctcgaag ttaccctacg tgcgcgctgg cttcaacgcc tggggcgcca gatgcaaact
     9841 gcctgatgag cgcgtgttct ctggcgcgcg cggattcaca gcagcgtgcc gcgcaggacc
     9901 gcctgagcta tgctgaaata gatgatcagc ccgcttacgt ccaccagagt tgccacgaat
     9961 ggtgcggagc aggttgcggg atcgagtcgg agtcttcgca gagccaacgg gagcatggcg
    10021 ccgaccaggc ttccgaaggc gaccacaccc accaggctca gtcccaccac cgtcgccacg
    10081 agcgcatagt ggtcggtgta gagcgacgcg cgattcggcc acagggcgat ccgcaggaag
    10141 ccgatgaccg caagcacggc gcccagggcg agccccaggg ccagttcccg gcgcaagatg
    10201 cgcagccaat cctgcaagtg gacatcaccc gtggccatgg cgcggatgac gagagtcgag
    10261 gcctgcgccc cggaattgcc gccgctgctg atgatgagcg ggatgaagag cgccagcacc
    10321 agggcgttgg cgaggtcccc ttcgtaatgt cccatggccg tggccgtcag catttgaccc
    10381 agaaacagca ggatcagcca gccgacgcgt ttgcggatca ggaccagcag atcggtgccc
    10441 agatacgacg catcgagggc ctgcatgccg cccatcttct gcatgtcctc cgtgacctcc
    10501 tcctcgacca gatcgagcac atcgtccacc gtgaccactc ccaccaggac ctcctgggaa
    10561 tccaccaccg gcagcatggt gcggtcgtat ttctggaact cggcgacggc cgtttcctga
    10621 tcgtctgtcg cgcgcagcga gatgacttgc ggttcgagca ggttcgcgac cggggtgtcg
    10681 agctcggcga ccaccaggtt ccgcaggcgc accactccgg ccagtcggcc ttgcgggtcc
    10741 acgacgaaca gttggttgag cgattcgcgg tgtcgcccga cccggcgcag gtgctcgagc
    10801 gcctccgcga ctgtccaatc gtgtttgacg gccacgtact cgggcgtcat gcgccggcca
    10861 acggactcct caggataacc caggagtgtg acggcaatcc tccgttcctc gggggagagg
    10921 agattcaaga gcttctgcgt ggccgcggcc gggagttcct cgaggagggc ggtgcggtcg
    10981 tccggctcca tctcgttgag gattgtcgcg acttgctcgt tgccgagcgc caggaccaac
    11041 cgctcctgtt cctcgacgcc gagctgttcg aagacatccg cggcgagttg atacggcagg
    11101 atgcgcaaga gcacagcgcg gtcctcggga gccaggtcgg tgaggatgtc cgcgatgtcc
    11161 tgcggcggga attcagcgag gatttggcgg agctggttga attcccgttc ttggatcaac
    11221 ccggtcagtt cgggtttcag caggcggcca atcatgcgtt cagaataggg ggttctccgt
    11281 ccggttgcat tccgaaagac gcgcgcgaga gagattttcc ccctacggcg cgtgaccagt
    11341 gctccggttt accccattcc ggcctgatct gtgacctctt ggacgctcga cgcgcagatc
    11401 ggcgaattgc atctcggcag ccagaagaag gcttaagacg gattaggagc caggcccatc
    11461 cattgcccgg tctcggcgct tcgcacagcg gcgtccatca ccgcctgaac gcgcgcgccg
    11521 tcgaagaaat cggggacgca cgggcgttgc tggcggatgg catcgacgaa ttcccaagcc
    11581 tggtcatagc ggaacgtgac caggggatcg ccgactcgag ggtcgcgcgg gctgcccggc
    11641 cagcgccagt attcctccgg gacgggaatc gtctcgagcc ccggtccgcc ggcccgcccg
    11701 gtctcgattt gattccagcg gccggtgatg aagacgaagc tgcgttcgct cccgttgacc
    11761 tcgaccgagt cgaggctgcg ccagctctcg ttgcgtccgg aggccagttt gctgctctcg
    11821 agcacgccgg tcgcgccgca ctcgaactcg gccagcagag ccacccaatc gtccaggtcg
    11881 ctgggcgcgt caccgcggac gggatggatg cgtttgagct gggcgacgag ccggcgcatc
    11941 gggccgatca ggagatgggc gaaatcgatg cggtgactga gcatgtcacc cagttcgccc
    12001 gtgcccgcca gggcgcggac ctggcgccag cccagattgc gcgtgcccca atcctgaagg
    12061 cggcaggagc ggtagtgata gggctggccg agatcgccgc gcttgacgag gtcatgcagg
    12121 tagcgcatgg cgggcacgaa cctgtaagta aaggccgtca tgtggcgcac gccggcccgc
    12181 tcggcggcct cggccatggc gaaggcgtcg gcagcgttca gggcgagtgg cttctcgcaa
    12241 aggagatgtt tgccatgagc gacggcggcc agggcgattc gaggatgggt gtggttgggc
    12301 gtcgcaatga tgacggcatc gacatcgtcg cgtcggacca cctcctcgaa ccgggtgctc
    12361 agggcggcga ctcctgtctg tcctcgagcc cgttcgagca cggcggggtt cgcatcgcag
    12421 agagccgtca cctgcgtgtc gggacacagg gcgagcccgg gcaggtggtt ttggagcgtg
    12481 atgctgccgc agccgatgat tgcgacgcga acaggtgttg tcatggataa tctcgatacc
    12541 gaacgaagca gagcaaattg cccccatgaa cctgaaaggt ctgtcgtaca actctcaact
    12601 ccccactccc cactctcaac tcctcggctc gtgggagggc ttggagatcc ggaaacggca
    12661 actggcgggc gtctcgacca ggtgcgcggt cacttcttgc gccaactctt gaactcgttg
    12721 agcaggtatc ggatcatatt tttttattct ttcttgatgt ggggtttgtc tgattctgct
    12781 gttggatcga ttcggacgcg gccctgtccg ggtcgcgcca tacgatccgt ccggcgacga
    12841 ttgtggcgag cgctttgccc ttcattggcc agccgtagaa cgggctgttg catgatttgc
    12901 cggccgtgtc ttcacggtgg aactcccact cgacatccgg atcgaagacg gtgacgtcgc
    12961 cgtcgctgcc cggtttgagc gacgctttcc cggtcagttt caacaatcgg gccgggccgg
    13021 ttgtgaagcg ggcgatgagc tcgctcaggc cgaggcgttt ggcgtggtaa agctgcatca
    13081 gggacagcgc cagctcggtc tcgaggccgg tgatgccgaa gggggcgtcg tcgaactcga
    13141 cgtccttttc gtagttgcag tgcggcgcat ggtcgctcga gatcacctcg atggtgccgt
    13201 cggccagcgc ttcgagcagc gcctcgcggt ccgccgccgc acggatcggc gggttcattt
    13261 tgacgtgcgt gtcgtaggcc ggccagaagg ggagggcgga ggaggatttg cccgaaatca
    13321 ggacggcttg gccgtccttg cgccagaatt cgtcgcttcc ggcgagggcc gcgtccgtga
    13381 gcgtgaagtg gtgcggacac gcctcgccgg agacgggcag gccctgttcc ctggccttgc
    13441 gcaggaggcg gacactgccg gcgctgctga tgtgctggca atggacgcgt gcgcccgtgc
    13501 gttcggccag caggatattg cgggccacga tcaactcctc gccggcggcc ggccagccgc
    13561 gcaggccgag catggcgctg taatagcctt cgtgggcgac accgtcggtg acgatcgagt
    13621 agtcctggca atggtccatc accagcagat cgaacatggt cgagtattcg agggcgtggc
    13681 gcatcagttc gttgttctgg atgcagtgtc cgtcgtcggt gacggcgacc acccctgccc
    13741 ttttgagcga acccatcggg cccagttcct cgccggccag ccctttgctg atggcgccgg
    13801 ccacatacac gttgacacgg ccttcgctgg cgacgcgttc atggaccagg gcgactgtgc
    13861 tggggctgtc gatgggcggc accgtgttgg gcatgcagac caccgatgta aatccgccct
    13921 tggcagcggc ggccgtgccg gtggcgatgg tctccttgct gctctgccct ggctcgcgca
    13981 gatgcacatg cagatcgatg agcccggggc agacgatcct gccggtggcg tcgaagcgat
    14041 cggcgtcggg cggcgcctgg gcggcggcac cctcgccaag agccgccact ttgccgtccc
    14101 ggatcagaat gtc
//
LOCUS       DAOQRO010000038        13896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3466_length_13896_cov_5.242685, whole genome shotgun sequence.
ACCESSION   DAOQRO010000038 DAOQRO010000000
VERSION     DAOQRO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3466_length_13896_cov_5.242685"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKK20_02615"
     CDS             <1..405
                     /locus_tag="PKK20_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98811.1"
                     /translation="PADRGRGFNIEWTLANAYPIRTNELLFPCSYYYYVTFYRTDDNS
                     TLKLRKVPEVRSPAKKAISPCFASMAREWNDIRKNTTTAGHGTKGMALLFVEGHAEFA
                     LYERLNAPFMDGTTPVYNLDWTVGGLAGEDLK"
     gene            complement(430..834)
                     /locus_tag="PKK20_02620"
     CDS             complement(430..834)
                     /locus_tag="PKK20_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98812.1"
                     /translation="MNATIVTALADRDQAIKHVLHSAFSLKPSSSSVNVTRLRSDRSI
                     RRDTFACTTGPGSPNPPRRGYQWTRKVGQKTITVALSRDQYQALKQALDNARTLWKTV
                     AEMELVSRQVLFETLPDTLCRKRLSKRVLGLI"
     gene            complement(831..2711)
                     /gene="murQ"
                     /locus_tag="PKK20_02625"
     CDS             complement(831..2711)
                     /gene="murQ"
                     /locus_tag="PKK20_02625"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNU98813.1"
                     /translation="MQQDRSGIRTETILLGIECGGTHTVAIAADAAGRQLHRLEAGPA
                     NQRLLTPTQLASHFRALANRLPAPSALGIGMAGVRESADRRLVQAAAATAWPGVPCWA
                     GNDLETALAAADAGTSAPDVTRVIVISGTGSCCYGRNPRGQTARVGGWGHLLSDKGSG
                     YDIALEAMQQAIRRFDLTGRWPDLGARLLLTLQLNEPNDIITWIHQAPKDEIAALAVQ
                     VFAAARGDPIARSAIQSAVRHLAADAVACARRLTRPGRPVEFVFVGGVLHRQKTFARA
                     VSLELRRLWPENASRLLKREGAWGAVAHARAIVPTLPHPARRPRTRSAASPDSPAPSC
                     PIPEATGQSPTEQRNPRSLDLDRRSLSSAIELMISEETRLPRALRAEAVRIERAIRLI
                     VRAFRRGGRLFYLGAGTSGRLGVLDASECPPTFGAPPEQVQGIMAGGHRALWAADEDV
                     EDDTEAGAKAILFRGVTRRDVVVGIAASGRTPFVWGALHRARDRGAATVLICFNPFLR
                     FARGERPDVVIAPNVGPEVLTGSTRLKAGTATKLILNMLTTLSMVRTGKAIGNLMVDL
                     NPSNEKLRERAVRIVCALTGAAPSVARTRLEQNGWQVKAALVHRALGEGRRLCHEKRC
                     QS"
     gene            complement(3113..7336)
                     /locus_tag="PKK20_02630"
     CDS             complement(3113..7336)
                     /locus_tag="PKK20_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98814.1"
                     /translation="MNSIPDRFKKPRLLSRGLAIAGLALLAGGAISQAGTFNADFNDG
                     IPIGVNLQGTAFNYDHSTGGVGGTGVLKLTDATASQQGGAIIDDFDNGEIIGGFEVTF
                     DLYIGSGNGADGMSFFFGDFADAAHSEEGPGTINGLTIAFDVYNNGGTPAEAPAIDLK
                     WNNVIQLHRLVGAASTTTGAQPIGSATTIRTQTTAGGAPVYWPVKIRVDTDGTLDLAY
                     NGQIVYTNVPIFRAITDFPISGIGARFGFGARTGGSYDNHWIDNLKITTFPLDANSGQ
                     PHMVSCLPLALQNATTGAPGSPVAGVMIDMKDYDYTIAEGSVTMTVNGTTVTPTLSKV
                     NDISRIIYRPASGYLAGGVNTIIVGYRTTSTPALNNSFTYSALVGTATAIDPFYALPA
                     ADTTKPGFKIKVHQMDPNRNPATGQYLNAERQIANGYIDPSTDQPFSNTALTDWLDTF
                     GNVLASADADGFFTAEGVINWNQDAPTAVGNFTSANGFTDQSIPGLTGNTGVNTTDRF
                     VASIETILELKAGGYRFGVNSDDGFRLSIGRGPGDVVGVQLGTAGDRSPIDSLMDVVI
                     PADGFYPVRLMYFETSGGASCEFFFVDSNGVRWLVNDLTAPQPIKAYRESAATPPYIS
                     RALPPIGYLWSFADQDLVLDVTDGASPLKAGSAVLTLNGTPQTITESKDGKVTTLVRS
                     SSMANLLPSGSVTAVLTYEHIPIGGAAQTVTNTWTFSVPPYTRPIPVANKVSSSDVSG
                     MGFHVRAHQIDRSRDANQGNGGRYTGQNGGAANMPRPEIQLANGYINRTNNMPYPNLA
                     VAGPEADGGYELGDVLNWNSGSGGLAANAGIFNADIAVPGVPGGGSSGSTAIGLDNTV
                     HEITTYLDLKAGVHILGLNVDDGWLCLSAPNPKDTLGTLLGWRNTPGGQNGNPLNNPN
                     GAFTVLVLEDGIYPFRILFWQGGGGINLEFLSIDRNFGTQILVNDVTGLYPSTTANPN
                     SVAPLESPIQAYSTYTGPVRPWTKFSVYPMPTHATLWQNTHQQSGPGPILVKVGAGNP
                     VDIANDEPRFNQALVNWPFGDAIGAIVADLGAGSVGMVLDDVSVTPTVTPIGGSTDVL
                     VMYVPNPPLAPMSTHTAGLVYANTTNYWTFTVSDYVNVPASAAVPASAVDLNAPGFRV
                     KVTQASAARPGGNTVAAAEAQLAGTPASVAVPGPEADGSYIDADIINWNVTMNPGGTP
                     AQIGNFQTLMTSQADEPVPGIPGTGLSGNLRFENITAEICAYLELPAGYQKFGINGDD
                     GWKVQIGMPGQTDGLVLFSRDRAAGARDIPFAFITPEAGFYPVRLVWYQGGGGGNLEF
                     FTYGPNNTKILVNDRNNPHAVKAYVPSAVQPTVSIALLADGKVRVTFEGKLQQSSSVS
                     PTNWQDVTGAVSPYEVSPTGTELYFRATR"
     gene            7749..9245
                     /locus_tag="PKK20_02635"
     CDS             7749..9245
                     /locus_tag="PKK20_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98815.1"
                     /translation="MIKLGSLPPTAGRILALGLGLCFFAHCLAQPIQLHPANPHYFFW
                     RGKPTVLITSGEHYGMVINPDLDYRRYLETLRVDGLNYTRVFAGAYVEKPGAFGIARN
                     NLAPGPGKFLAPWARSPVAGYAGGGNKFDLARWNPDYFKRLTDFVQDAGQRGIVVEVT
                     LFSSIYNNEQWSFSPFNPTNNVNGTRLSDWRRLHTRTNDGLLAVQEAYVRQIVRALNS
                     YDNVFYELQNEPWSDHHTMGDLINPYLPDTPEFPNAVEITTAPSLEWQATVASWITRE
                     QSVLPQRHLIAQNIANFRLPVRETDIAPGVGILNFHYAYPEAVAWNYGLARPIGYDET
                     GFAGSADDTYRREAWAFLMAGGALFNHLDYSFTAGAEDGTDTALNGPGGGSPALRRQF
                     NVLGEFIRSFNFVALRPDSQVVRKSPGVVTRVLSQPGVAYSLYARGRSPCVIELELPP
                     GPYRADWIETRAGRVLGSEAVAHRGGPCRLQSPAFEDDIALRLLRAVR"
     gene            complement(9589..10482)
                     /locus_tag="PKK20_02640"
     CDS             complement(9589..10482)
                     /locus_tag="PKK20_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98816.1"
                     /translation="MEDAAFAALRWTRLRGSVRNLHAAGAREHPLWILHSLLMFCVPE
                     AAKNGQSRFLFRPLAEPGDVIERDRLYPFAVFFPGDDLALARACAAGLERHLSDKRHH
                     FALVELESPQVRSLADLEDAASRWPERATEVCLEFETPFAYTPADGRRPWWLAASDLG
                     RLLAHRFQHFFVCPAPPPRADWATLTTLCHFWRFVEHRHRAKSGRGTKTIAGNVGPLF
                     LRGTAQALASVRQWLLLGTEIGAGLKLGPRGHYRLRFDRPWFDPIFCDPATYTRALDD
                     LKGRSGVFIRPAKEATAAQSA"
     gene            complement(10617..>13896)
                     /locus_tag="PKK20_02645"
     CDS             complement(10617..>13896)
                     /locus_tag="PKK20_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HNU98817.1"
                     /translation="LPGLFTVVSPWLCAVLLVAGHALLAILPLLRDATFQPGHMQPLA
                     VSLAVSLVALFALHALARKLARPTAAALVAASSQALHLVEAGEQAAWNEHAQEQQRIE
                     EEARSKNSVLEDRWHRAVDAAAAQRTELKASLGTKLHRALGRGDRLRLRASDRLQREH
                     AARAAALNQAAAEQRTALEQERERRVSELRTALQDSSQALVRDWAQVTGTAYAAFEGL
                     RNAAAESFPDWASRSWDDWSPPSASVSAAPFASVTLDVAAYVGGLPSDPRLVLPGPAQ
                     FDLRLLLKHPEQGSIVIETQAWGRAKALAALNAMVLRLLMSAPPGRVLFTILDPVGLG
                     ETFAGLMHLSDHEDRLINRRIWTQPDQIEQRLADLNEHIEKVTQLYLRNEYATIADYN
                     AQAGRIAEPYHFLVAADFPVNFSESAAKRLLSIASSGPRCGVFLLLHWDRRKPLPAEA
                     APEDLRKAGARIAAVNQAFELEDPRLEGIPLRLEEPPPPETVTTILTNLGQASVDSNR
                     VEMPFAEIAPDTENLWTLDTTHELRVAIGVTGATKLQYLALGKGTRQHALVAGKTGSG
                     KSTLFHVLVTNLALWCSPHAIEFYLVDFKKGVEFKCYGERRLPHARVVAIESDREFGL
                     SVLERLDQELQRRGDLFRQAGVQDLPAYHRASPDNRLPRALLIIDEFQEFFTEDDRVA
                     QNAALLLDRLVRQGRAFGIHVMLGSQTLGGAYTLTRTTLGQMVVRIALQCNQPDAMLI
                     MDDDNAAPHLLSRPGEAIYNDAAGAIEGNSPFQIVWLPEAERNVCLETIQRRAERVTG
                     LPPPPVVFEGNAPADVRENRLLEELLRARPQERLVAPRIYLGAPNSIKGPTEIALHRQ
                     SGNHVLIVGQYDEPALAMLGLALIALAAQHPPGTARFLLLDATPAAAPHRAFLERVTA
                     LIPHDVRWVGNTQLDDALGVVGAELAARSDPEAAAAAPSVYLCIHHLERFKRLRFEED
                     FSLALDSEEKTIDPGRELNRLLCEGAHVGLHALCLCDTYNNVSRFFSRRALTEFELRI
                     LFQMSPSDSASLIDSPKASTLGLHRAVLHNAQAGSLETFRPYALPDPAWLDRAKTLLS
                     DRTA"
BASE COUNT         2329 a         4243 c         4819 g         2505 t
ORIGIN      
        1 ccggcggacc gcggacgcgg cttcaacatt gagtggactc ttgcgaatgc gtatccgatc
       61 cgcaccaacg aactcctctt cccctgctct tactactact acgtcacgtt ttatcgcacc
      121 gacgacaaca gcaccctgaa gcttcgcaaa gtccccgagg tccggtcccc tgccaagaag
      181 gcgatctccc cctgcttcgc gagcatggcg cgcgagtgga acgacatccg caagaacacc
      241 accactgcgg gccacggcac caagggcatg gcgcttctgt tcgtcgaggg ccatgccgag
      301 ttcgccctgt acgaacggct caacgcgccg ttcatggacg gcacgactcc cgtttacaac
      361 ctcgattgga ctgtcggcgg cctcgccggc gaagatttga aatagggcca gggcagaatg
      421 gcgcttagct tagataaggc ctaaaactct cttggacagg cgcttgcgac agagggtgtc
      481 ggggagcgtc tcaaaaagta cctggcggga gacgagctcc atctcggcga cggtcttcca
      541 gagggtgcgg gcgttgtcca gcgcctgctt cagcgcttgg tactggtcgc gactcaaagc
      601 gacggtgatg gtcttttgac cgactttgcg cgtccactga taaccacgac ggggtggatt
      661 tggagacccg ggacctgttg tgcacgcaaa ggtgtcgcgc cggattgagc gatcggatcg
      721 gagccgagtg acattcaccg acgaggaaga cggttttagg ctgaaggcgg aatggagtac
      781 gtgcttgatt gcctggtcgc gatcggcgag cgcggtcacg atggtggcgt tcatgattgg
      841 cagcgcttct catggcacaa gcgccgccct tcgccaagag cgcgatggac cagggcggcc
      901 ttcacctgcc atccgttctg ttcgaggcgt gtccgcgcca cggatggcgc ggcgccggtg
      961 agtgcgcaca caattcgcac cgcgcgctcg cgcagttttt cgttcgacgg gttcaggtcc
     1021 accatcaggt tgccgatcgc ctttccggtg cggaccatcg agagggtggt cagcatgttg
     1081 agaatgagct tggtggccgt tcccgccttc agtcgcgtcg agcccgtcaa cacctcgggg
     1141 cccacattgg gcgcaatcac gacatccggg cgctcgccgc gcgcgaacct caggaacgga
     1201 ttgaagcaga tcaacacggt cgccgcgccc cggtccctgg cgcggtgcag agcgccccag
     1261 acgaacgggg tgcgtccgct ggctgcaatc cccacaacca cgtcgcggcg tgtcacgccg
     1321 cggaacagga tcgccttcgc cccggcctcc gtatcgtcct cgacatcctc gtcggccgcc
     1381 cacagcgcgc ggtggccgcc ggccatgatg ccctgcacct gttcgggggg ggcgccgaat
     1441 gtcgggggac attcgcttgc atccagcaca cccaaacgcc cgctggtccc ggcgcccaga
     1501 tagaacaaac gcccgccgcg gcggaaggcc cgcacgatga gcctgatcgc cctctcgatc
     1561 cgcaccgctt cggcccgcaa ggcgcgcggc agcctggtct cctccgagat catcaactcg
     1621 attgcggacg agagcgatcg ccggtccagg tcgagggacc gcggattgcg ttgttcggtc
     1681 ggcgactggc ccgtagcctc ggggatgggg caagaggggg cggggctgtc gggggacgca
     1741 gccgagcgag tgcggggacg ccgagccgga tgcggcaggg tggggacgat ggcgcgggcg
     1801 tgggcgacgg cgccccaggc cccttcgcgt ttcaggagtc ggcttgcgtt ttcgggccag
     1861 aggcgtcgga gttcgaggct caccgctcga gcgaacgtct tctggcggtg aagaacgccc
     1921 cccacaaaaa cgaactcgac tgggcgaccg ggccgggtca atcgccgggc gcacgccacc
     1981 gcgtccgccg ccaggtgccg gaccgcggat tggatggcgc ttcgggcgat aggatcgccc
     2041 cgcgcggcgg cgaaaacctg gaccgcgagg gcggcgatct cgtccttggg cgcctgatgg
     2101 atccatgtga tgatgtcatt gggctcgttg agttgaagcg tgagaaggag ccgcgcgccg
     2161 aggtcgggcc agcggccggt gagatcgaat cggcgaatgg cctgctgcat ggcctcgagc
     2221 gcgatgtcgt atccgctgcc tttgtcgctc agcagatgtc cccagccccc gactctcgcc
     2281 gtctggccgc gcgggttgcg tccgtagcag cacgaacccg ttccgctgat gacgatgacg
     2341 cgcgtgacat cgggtgcgga cgtacccgcg tcggcggcgg ccagagcggt ttcgaggtcg
     2401 ttgccagccc agcacgggac acccggccat gcggtcgcgg ccgcggcctg gaccaggcgg
     2461 cggtcagccg actcgcgcac gccagccatg ccgatgccca aggcggacgg agcgggcagc
     2521 cggttggcga gcgcgcggaa gtgggaggcc aactgggtcg gggtcagcag gcgctggttg
     2581 gccgggccgg cttcgagtcg atggagttgg cgcccggccg catcggcggc gatggccacg
     2641 gtatgtgtgc cgccgcactc gattccgagc agtatggttt cagtgcgaat acccgagcgg
     2701 tcctgctgca tgggtccgag tattcgaggt ggctcgggct tggcgagtcg aaaagggggg
     2761 tgtgccgcca gcctcgcacc cgagcgtctg gcgcgatcgc gttcctgcga actcgagaga
     2821 ctcgaggcga ggcatggctt gatatggcgg ctgcgcagtt ccggatttta gcgcggtcag
     2881 cccggttgtt cacacccttc gcgaggaatc gtaaccgggc tagttggcaa aaccgcgccc
     2941 agaaaggcaa ccagagatac tccgtcggtc gtgacggcgt ccttccacac gaacagtgtc
     3001 tcggattgct ctgtccctca agccgggcgc ggttttgctt ttctaatcgg catggcagtc
     3061 ctcttgcgaa gtctgactgc catgtgcaca gacgggatgt ggaatatgcg ggttagcgcg
     3121 tggcgcggaa gtacagctcg gttccggtcg gactgacctc gtacgggctg acggcgccgg
     3181 tcacgtcctg ccagttcgtc gggctgaccg acgacgattg ctgcagcttg ccttcgaagg
     3241 tcacccgcac cttgccgtca gccagcagcg cgatggagac agtgggttgg accgctgaag
     3301 gaacgtacgc cttgactgcg tgcggattgt tgcggtcgtt gacgagtatc ttggtgttat
     3361 tcgggccgta ggtgaagaac tcgaggttcc caccgccgcc gccctggtac cagaccagtc
     3421 ggactgggta gaagccggcc tcgggcgtga tgaaggcgaa cgggatgtcg cgcgctccgg
     3481 cggcgcggtc gcgggagaac aggacgaggc cgtcggtctg gccgggcatg ccgatctgga
     3541 ctttccagcc gtcgtcgccg ttgataccga acttctggta gccggcgggc aattcgaggt
     3601 acgcgcagat ttcagcggta atgttctcga accgcagatt gccggagagg ccggtgccgg
     3661 ggatgcctgg gacaggctcg tcggcctggc ttgtcatgag cgtctggaag ttgccgatct
     3721 gggcgggcgt gccgccggga ttcatggtga cgttccagtt gatgatgtcg gcgtcgatgt
     3781 agctgccgtc cgcttccggt cccgggacag cgacgctggc cggggtgccg gccagttgcg
     3841 cctcggcagc ggcgacggtg ttgccgcccg ggcgcgcggc ggaagcctgg gtgaccttga
     3901 cgcggaagcc cggggcattg aggtcgacgg cggaggcggg gacagcggcg ctggcgggga
     3961 cattcacgta atcgctgacg gtgaacgtcc agtaattggt cgtgttggca tagaccaggc
     4021 cggcggtgtg ggtggacatg ggtgccagcg gcgggttggg cacatacatc accaacacat
     4081 ccgtcgaccc gccgatcggg gtgacggtgg gggtgacgct gacgtcgtcc agcaccatgc
     4141 cgacgcttcc ggcgccgagg tccgccacga tggcgccgat ggcatcgccg aacggccagt
     4201 tgaccagggc ctgattgaac cgcggctcgt cattggcgat gtccaccgga ttgcccgcgc
     4261 cgactttgac gaggatcgga cccgggccgc tctgttggtg agtgttttgc cagagcgtcg
     4321 cgtgggtcgg catcgggtag acggagaact tggtccaagg ccggaccggg cctgtatagg
     4381 tgctataggc ctgaatcggc gattcgagcg gggcgacgga gttcggattg gccgttgtcg
     4441 acggatagag cccagtcaca tcgttgacga gtatctgggt cccgaagttg cggtcgatgc
     4501 tcaggaactc gaggttgatg ccgccgccgc cctgccagaa caggatgcgg aacgggtaga
     4561 tgccgtcttc gaggacgagc acggtgaagg cgccgttcgg gttgttgagg gggttgccgt
     4621 tctggccgcc cggggtattg cgccacccca gcagggtgcc gagcgtgtct ttcggatttg
     4681 gcgccgagag gcagagccag ccgtcatcga cattcaggcc gaggatatgc acgcccgcct
     4741 tgaggtcgag gtaggtggtg atctcgtgga cggtgttgtc cagcccgatc gccgtcgaac
     4801 cggaactgcc gccgccgggc acgccaggca cggcgatgtc ggcattgaag atgccggcgt
     4861 tcgcggccag gccacccgat ccggaattcc agttcagcac gtcgcccaac tcgtagccgc
     4921 cgtccgcttc cgggccggca acggccaggt tgggataggg catgttgttg gtgcgattga
     4981 tgtagccgtt ggcaagttgg atttccggac gcggcatatt cgccgctcca ccgttttgac
     5041 ccgtgtaacg tccaccgttg ccctgatttg cgtcacggga gcgatcgatc tgatgggccc
     5101 gcacgtggaa tcccatgccg ctgacatccg aagacgacac cttgttcgcg accgggatgg
     5161 ggcgggtgta gggtggcacg ctgaaggtcc aggtgtttgt caccgtttgg gcggcgccac
     5221 caatcggtat gtgttcatag gtgagaactg cggtcacgct gcccgagggg aggagattcg
     5281 ccatcgaact cgagcgcact agcgtggtga ccttgccatc cttgctttcg gtgattgtct
     5341 ggggcgtgcc gttcaaggtc agcaccgccg acccagcctt gagcggcgaa gcgccatcgg
     5401 tcacatcgag gaccagatcc tgatcggcaa acgaccagag gtatccgatc ggcggcaacg
     5461 cgcgcgagat gtagggcggc gtggcggcgc tttcgcggta agccttgatg ggctgcggcg
     5521 ccgtcagatc gttcaccagc caccggacac cgttggaatc gacgaagaag aactcgcaac
     5581 tggcgccacc gctcgtttcg aagtacatca accggacggg gtagaagcca tccgcgggga
     5641 tgacgacgtc catgagcgaa tcgatcgggc tgcggtcgcc tgcagtgccg agctggacgc
     5701 cgacgacgtc gccggggccg cgtccgatgg acaggcggaa accgtcgtcg ctgttgactc
     5761 caaagcggta gccgcccgcc ttgagttcga ggatggtctc gatcgaggcg acgaagcgat
     5821 cggtcgtatt cacgccggta ttgcctgtga ggcccgggat gctctggtcc gtaaacccgt
     5881 tcgccgatgt gaagttcccg actgcggtgg gggcgtcctg gttccaattg atcacgcctt
     5941 cggccgtgaa gaaaccgtcg gcatcggccg acgccagcac attgccgaag gtatcgagcc
     6001 aatcggtgag cgcggtgttg gagaagggtt ggtcggtgga gggatcgatg tagccgttcg
     6061 cgatctggcg ctcggcattg agatactggc cggtcgccgg attgcggttc gggtccatct
     6121 ggtggacttt gatcttgaac cctggtttgg tcgtgtccgc agccggcagg gcgtagaagg
     6181 gatcgatggc cgttgcggtt ccgaccaggg cgctgtaggt gaaggagttg ttcagcgccg
     6241 gcgtgctggt ggtcctgtag cccacaatga tcgtgttcac gccgcccgcc agataacccg
     6301 aggccggccg gtagatgatt cgggagatgt cattcacctt gctcagagtg ggcgtgaccg
     6361 tcgtgccgtt gacggtcata gtgaccgatc cctcggcgat cgtgtagtca tagtccttca
     6421 tgtcgatcat gacgcccgcc acggggctgc ccggggcgcc tgtggtggcg ttctgcaacg
     6481 ccaggggcag acacgacacc atgtgcggct ggcccgaatt ggcgtcgagc gggaaggtgg
     6541 tgatcttgag gttgtcgatc cagtggttgt cgtaggaacc gccagtgcgc gcgccgaagc
     6601 cgaaccgcgc gcctatcccg ctgatcggga agtcggtgat ggcgcggaag atcgggacat
     6661 tggtgtacac gatctggccg ttgtaggcga gatcgagcgt gccatccgta tcgacccgta
     6721 ttttcaccgg ccagtagacc ggagcgccac cggcggtggt ctgggtgcgg atggttgtgg
     6781 cggagccgat cggttgggcg cctgtcgtgg tcgaggcggc tcccaccagg cgatggagtt
     6841 ggatgacgtt gttccatttg aggtcgatgg ccggcgcttc ggccggtgtc ccgccgttgt
     6901 tgtaaacgtc gaacgcgatc gtcaggccgt tgatggtgcc cggcccttcc tcagagtggg
     6961 cggcatcggc gaaatcgccg aagaagaacg acatcccgtc cgccccgttg ccgctgccaa
     7021 tgtagagatc gaaggtgacc tcgaatcccc cgatgatctc gccattgtcg aaatcatcga
     7081 tgatcgcccc gccctgctgg ctggcggtcg cgtcggtgag cttgaggacg cccgtgccgc
     7141 ctacgccgcc ggttgaatgg tcgtaattga atgccgttcc ttggagattg acgccaatcg
     7201 ggatgccgtc gttaaagtcg gcgttgaatg tgccggcctg gctgatggct cctccggcca
     7261 gcagcgccaa gccagcaatg gccaggcccc gggacagcaa cctcggcttt ttgaatcgat
     7321 cgggtatgga gttcatgacg tgattgggga cagatgctcg agaccgagca tcgggttgtt
     7381 tcctccgatt taggttatgg gtacttgtga agcctccgcc catcgtcgcc ctcgatccgt
     7441 gttcgccgcg gacaacggca ctcggcaatc caggatcgcc aggatcggtt caacgctgtc
     7501 gggctcaggc ggcggaagta tgggcaattc cgagccgttg tcaagccgct ccaaatgaaa
     7561 agaccgtgcc cggacagcgc caattcatag agtggagtcg cgacgccggg aaacaccgcc
     7621 ttcacttgac atggcgggcg acgagccttc ctccccactc ggtccttttc cctttcatga
     7681 gttggccctg gccggacagg cgcagacttg cctcgatggc atccacgctt tagggtctgt
     7741 acaccgcaat gatcaagctc ggttcactcc cgcccaccgc gggtcggatt ctggctctgg
     7801 ggctcgggtt atgttttttc gcgcactgtc tggcccagcc gattcaactg cacccggcca
     7861 acccgcatta cttcttctgg cgcggcaagc cgacggtcct gatcacgtca ggtgagcact
     7921 acgggatggt catcaacccg gacctggatt accgacggta tctcgagacc ctgagggtcg
     7981 atggcctcaa ttacacgcgc gtcttcgccg gggcctacgt cgagaaaccg ggcgccttcg
     8041 gcatcgcgcg caacaacctc gcgcccgggc cgggcaagtt cctggcgccc tgggcgcgga
     8101 gtccagtcgc cggttacgcc ggagggggca acaagttcga tctggcacgg tggaatccgg
     8161 actacttcaa gcgtctgacc gattttgtgc aggatgcggg gcagcggggg atcgtggtcg
     8221 aggtgactct cttctcgtcg atctacaaca acgagcagtg gtcgttcagc ccattcaacc
     8281 cgacgaacaa cgtgaacggg acgcggctga gcgactggcg gcggctgcac acgcggacga
     8341 acgacggtct gttggcggtg caggaggcct atgtgcgcca gatcgtccgg gccctgaaca
     8401 gctacgacaa cgtcttctac gaactccaga acgaaccgtg gtccgatcat cacacgatgg
     8461 gcgacctcat caatccctac ctgccggata cgccggagtt cccgaatgcg gtcgagatca
     8521 cgaccgcgcc gtccctcgag tggcaggcga cggtggcgtc gtggatcacc cgcgaacaga
     8581 gcgttctgcc ccagaggcat ctgatcgccc agaacatcgc caatttccgg ctgccggtgc
     8641 gcgagacgga catcgcgccc ggggtcggta tcctcaattt ccactatgcc tatccggagg
     8701 cggtcgcctg gaactacgga ctcgcccggc cgatcgggta cgacgaaacg ggctttgccg
     8761 gcagtgccga cgacacgtat cggcgggaag cgtgggcgtt cctgatggcc ggaggcgcct
     8821 tgttcaacca cctcgactac tcgttcaccg ccggcgcgga agacgggaca gacaccgcgc
     8881 tgaacggtcc ggggggcggc agcccggcgc tccgccgcca gttcaacgtg ctgggtgaat
     8941 tcatccgaag tttcaacttc gtcgcgttgc gtcccgattc ccaggttgtc cggaagtcgc
     9001 cgggggttgt cacacgagtc ctgagccagc ccggcgtcgc ctactccctc tatgcgagag
     9061 gccggtctcc gtgcgtcatc gaactcgaat tgcctccggg tccctatcgg gccgactgga
     9121 tcgagacgcg cgcagggcgg gtcttgggct ccgaggccgt ggcgcaccgg ggcggtccgt
     9181 gccggctcca gagcccggcg ttcgaagacg acatcgcgct gcggctgctg cgcgccgtgc
     9241 gatgagtgga gaaagtggtg gatggttgat gattgtgttc gggtcgtact gctgttcggc
     9301 cgtggagatg ccctcgtcgt cctgaaaaag gagttccgcc tccctcggcg tcacctcccc
     9361 catgcttcga gcgcgctttg cggctcgtgg ccgcgcctcg gcaattcgcg gtgaatttca
     9421 tttgcccacg cgaatgccga ctcggacgac actttcgagc ttagggtccg tgcaagaatc
     9481 aattccggtt ttggcgggag cgccaccggg ccagatgcga ggcccaaggg agggaatgta
     9541 tcccctgact ggcccgcgtt catgccgacg gcatcctggt ttggcgcttc aagcgctctg
     9601 agccgcagtc gcttccttgg ccggacgaat gaacacgcct gacctgcctt tcaagtcatc
     9661 cagcgctcgc gtgtacgtgg ccgggtcgca aaaaatcgga tcgaaccagg gccgatcgaa
     9721 acgcagccgg tagtggcctc gaggacccag tttcagtccc gctccgatct cggtccccag
     9781 caggagccac tggcgcacgc tcgccagcgc ttgggccgtg ccgcgcagaa agagcggacc
     9841 gacgttgccg gcgatcgtct tggtcccccg accgctcttg gcccgatgcc ggtgctcgac
     9901 gaacctccaa aagtggcaaa gcgtggtcag agtcgcccaa tcggcccgag gcggcggggc
     9961 ggggcagacg aagaaatgct ggaaccggtg cgccagcagc cggcccaggt ccgacgctgc
    10021 cagccaccac gggcgtcggc catccgccgg ggtataagca aacggagtct cgaactcgag
    10081 acaaacttcg gtggcgcgct caggccaccg cgacgcagcg tcctcgaggt cggccaacga
    10141 ccggacctgc ggggactcga gttccacgag ggcgaagtgg tggcgtttgt cgctcagatg
    10201 acgctcgagt cctgccgcgc atgcacgggc cagcgcaagg tcgtccccag gaaagaacac
    10261 cgcaaagggg tagagacggt cccgctcgat gacgtcgccc ggctccgcca gggggcggaa
    10321 gaggaagcgg ctttgaccgt tcttggccgc ctcgggcacg cagaacatca gcaggctgtg
    10381 cagaatccaa agcggatgct ctcgagctcc cgctgcgtgg aggttgcgca cactgccccg
    10441 caaccgcgtc caccgcagag cagcaaatgc ggcatcttcc atgctgccgc gcatagggcg
    10501 gacagtctgg cgaggcgcgc ctgagaagac aagcgcagtc ctctgcccaa cacgcgcatt
    10561 ccacatcccg tcagtgcacg gggcgggccg atctcgtatg atgtccgcca tgcgggttag
    10621 gcggtgcgat ccgagaggag ggttttggcg cggtcgagcc aggccggatc gggcagggca
    10681 taggggcgga aggtttcgag cgatccggcc tgggcgttgt gcaggacggc ccggtgcagg
    10741 ccgagcgtgc tggccttggg actgtcgatg aggctggccg agtcgctggg gctcatctgg
    10801 aagaggatgc ggagttcgaa ttcggtcaac gcgcgcctgc tgaagaaccg gctcacgttg
    10861 ttgtaggtgt cgcagaggca gagcgcgtgg agaccgacat gcgccccttc gcacaggagc
    10921 cggttgagtt cgcgccccgg atcgatcgtt ttctcctcgc tgtcgagcgc caggctgaaa
    10981 tcctcctcga accgcagccg cttgaagcgc tcgagatgat ggatgcagag atagacggac
    11041 ggagcggccg cggcggcttc gggatcgctg cgcgcggcca gttcggcgcc gaccacgccc
    11101 agggcgtcgt cgagttgggt attccccacc caacgcacgt cgtgggggat taaagccgtc
    11161 acccgttcga ggaaggcccg atgcggcgcg gccgcggggg tggcatccag gaggaggaac
    11221 cgcgccgtgc ctggcgggtg ctgcgcggcc agagcgatca gggcaagccc gagcatcgcc
    11281 agggcgggct cgtcgtattg gccgacaatc aggacgtggt tgccgctctg acgatggagc
    11341 gcgatttcgg tggggccttt gatcgagttg ggcgcgccca ggtaaatgcg gggcgcgacg
    11401 aggcgctcct gtggccgggc gcgcagcagt tcttcgagca agcgattctc gcgcacgtcg
    11461 gcgggtgcgt tgccctcgaa gaccaccggc gggggcggca atcccgtgac ccgctccgcg
    11521 cggcgttgga ttgtctcgag acagacgttt cgttcggctt cgggcagcca gacgatctgg
    11581 aaggggctgt tgccctcgat cgcgccggcg gcgtcgttgt agatcgcctc gcccggacgc
    11641 gacaggaggt gcggcgcggc gttgtcgtcg tccatgatca acatggcgtc gggctggttg
    11701 cactggagag cgatgcggac gaccatttgg ccgagagtgg tgcgtgtgag cgtgtacgct
    11761 ccgcccaggg tttgcgaacc gagcatgacg tggatgccga aggcgcggcc ttgccgcacc
    11821 aggcggtcga gcaggagggc ggcgttttgg gccacgcgat cgtcttcggt gaagaactcc
    11881 tggaactcgt cgatgatcag gagggcgcgc gggaggcggt tgtccgggga ggcccggtgg
    11941 taggccggca ggtcctggac gccggcctgc cggaacaggt cgccgcggcg ctgcagttcc
    12001 tggtcgaggc gttcgaggac gcttagcccg aactcgcgat cgctttcgat ggcgaccacg
    12061 cgggcgtggg ggagccggcg ctcgccatag cacttgaact cgacaccttt cttgaagtcg
    12121 accaggtaga actcgatcgc gtgcgggctg caccagaggg cgagattggt gacaaggacg
    12181 tggaagaggg tcgatttgcc cgagcccgtc ttcccggcga cgagcgcatg ctgtcgggtc
    12241 cctttcccga gggccaggta ctggagcttg gtcgccccgg taacgccgat cgccacgcgc
    12301 aactcatggg tggtgtcgag tgtccagaga ttctccgtgt cgggtgcaat ctcggcgaac
    12361 ggcatctcga cgcggttcga gtccacgctc gcctgtccga ggtttgtgag gatggtcgtg
    12421 accgtctcgg gcggcggcgg ctcttcgagg cgcaaaggta tcccttcgag gcgcgggtcc
    12481 tcgagttcga aagcctggtt caccgcggcg attcgcgccc cggctttgcg caagtcctcc
    12541 ggcgccgcct cggccgggag cggtttgcgc cggtcccagt ggagcagcag aaacacgccg
    12601 caacgcggtc cgctcgatgc gatgctcagg aggcgcttgg cggcggactc gctgaagttg
    12661 accgggaaat ccgcggcgac gaggaaatga tacggctcgg cgatgcgccc ggcctgggcg
    12721 ttgtaatcgg cgatcgtggc gtactcgttg cgcaaataga gttgcgtcac tttctcgatg
    12781 tgttcgttga ggtcggccag gcgctgctcg atctgatcgg gttgggtcca gatgcggcgg
    12841 ttgatcaggc gatcctcgtg atcgctgagg tgcatcagcc ccgcaaaggt ctcccccagg
    12901 ccgaccgggt ccaggatcgt gaacaacacc cgtcctggcg gggcgctcat caacagtcgc
    12961 aggaccatgg cgttcagagc cgccaaggcc ttggcgcgtc cccaggcctg cgtctcgatg
    13021 acgatcgagc cctgctcggg atgcttcagc aagagtcgca gatcgaactg cgcaggcccg
    13081 ggcaacacca ggcgcggatc ggacggcaga ccgccgacat acgccgcgac gtccagagtc
    13141 acagaggcga atggagccgc ggagactgat gccgagggcg gcgaccaatc atcccaagac
    13201 cggcttgccc agtccgggaa gctctccgca gcggcgttgc gcagcccctc gaatgcggca
    13261 taggccgtcc cggtgacctg cgcccaatcc cgcaccaggg cttgcgacga gtcttgaagc
    13321 gccgtccgca attcgctcac ccgccgctcg cgctcctgtt caagggctgt ccgttgctcg
    13381 gcggcggcct gattcagggc cgcggcgcgc gcggcgtgtt ccctttgaag ccgatccgag
    13441 gcgcgcagcc ggagccggtc gccgcgaccc agtgcccggt gaagtttggt ccccagactg
    13501 gccttcagtt cggtccgttg cgcggcggcg gcgtcgacag cccgatgcca gcggtcttcg
    13561 aggactgagt ttttcgaacg ggcctcctct tcgatgcgct gttgctcctg ggcgtgctcg
    13621 ttccacgccg cctgttcccc ggcctcgacc aggtgcagcg cctggctcga tgcggccacc
    13681 agggccgcgg cggtcgggcg ggcaagcttc cgggccaacg cgtgcaaagc gaaaagggcg
    13741 acgagcgaga cggcgaggga gacggccagc ggctgcatgt ggccgggctg gaacgtggca
    13801 tcgcgcagga gcgggagaat ggcgagcagc gcatggcctg ccaccagcag aacggcgcag
    13861 agccagggcg acaccaccgt gaagaggccc ggaagc
//
LOCUS       DAOQRO010000039        13879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3473_length_13879_cov_3.988932, whole genome shotgun sequence.
ACCESSION   DAOQRO010000039 DAOQRO010000000
VERSION     DAOQRO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3473_length_13879_cov_3.988932"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="PKK20_02650"
     CDS             <1..1725
                     /locus_tag="PKK20_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HNU98818.1"
                     /translation="GVHHAGLLPKYRVLVEQLAQRGLLKIICGTDTLGVGINVPIRTV
                     LFTRLCKYDGQKTGILSARDFHQISGRAGRKGFDDRGWVVAQAPEHVIENLKLEEKSA
                     KQGRKFVKRRPPERNFVNWDKNTFTRLIGALPERLTSRFQVSHGMLLNVLSRNSDGCR
                     AMRQLIRDSHESPKGKRAHRRRAWQLFRSLLERKIVEFIPRADRAEADHPDRKLRVNV
                     DLQDDFSMDQALSLYLIETVPTLDPAAPDYALDLLTLVESILEDPDIILRKQLDRVKS
                     QAMADMKARGLEYQERMDELEKLEYPKPNRDFIYSTFNAFANKHPWVGQENIRPKSIA
                     REMFEQFRSFGDYIRDYELQRSEGLLLRHLNSVFKVLAQTVPDAAKTDSIREMELYLS
                     AMIRQVDSSLLDEWEKMRGTKLAQQSEPATEVRPPGADDAAADITRNTRAFTAAIRTV
                     IFTFLRGCVVGDYQAALAALAEPTEGAAAAQSPQDTWTPDRLDQTLELYFAEHQQICL
                     DPNARNTRHTYVLPSEDQRTWRVQQMLIDPEEHNDWVAEFDVDLLKSRELQAPVVRLH
                     RIGLLTRP"
     gene            complement(1861..3387)
                     /locus_tag="PKK20_02655"
     CDS             complement(1861..3387)
                     /locus_tag="PKK20_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNU98819.1"
                     /translation="MLAKVGSAAVNGIEAYAVEVEVNSGWGDTTIVIVGLPDAAVKES
                     RDRVTTALSNSGFRFPLGRMTINLAPADVKKEGPSFDLPIAVGMIAASEQMLLDQLDN
                     FLIIGELALTGAVRPVKGVLPIALRAKADGKTGLLVPPDNAAEAAVVAGLNVIPVQNL
                     REAVAFLEGEITLAPVRLDMARLFAQPIEDELDFADVKGQESVKRALEIAAAGGHNVL
                     LIGPPGTGKSMLAKRLPTILPPMTLEEALETTKIHSIVGQLNGQALVTRRPFRTPHHT
                     ASDAGLLGGNIVPTPGEISLAHNGVLFLDELPEFKRSVLETMRQPLEEGRVTISRAAG
                     TMTFPAAFMLVAAMNPTPDGKMPGESRCSPREIQNYLGRISGPLLDRIDLHVEVPPVK
                     FRDMTAEGMGEPSACVRERVVAARKRQQERFAGRSRVPCNARMTSRDLKRWCALDETT
                     LEMLKVAMTDLDLSARAYDRILKVSRTIADLADSDRIVPDHLAEAIQLRSLDRQVWGR
                     "
     gene            complement(3550..4233)
                     /locus_tag="PKK20_02660"
     CDS             complement(3550..4233)
                     /locus_tag="PKK20_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNU98820.1"
                     /translation="MKMHLLKSTLTLAVALACAMLAQAADDLIKYAARPGCLVSIDGT
                     STIHDWTTKGAIIGGSFEVEPEFLTDKTLKSVKSLTTKEVNPKVQVKIPVKSLKSQVL
                     LGASKMDEVMQEAMRMKDNPDILYTLKEMIVKGDVPASGSPVKFDTKGDLTISGVTNT
                     IDMEVTMTRVADDRIKFSGSKVLKMTDYKVPPPAPNILGMSPIKTGDEVTIKFEWLLA
                     LQKTDAAAK"
     gene            complement(4364..5749)
                     /locus_tag="PKK20_02665"
     CDS             complement(4364..5749)
                     /locus_tag="PKK20_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98821.1"
                     /translation="MKSDRMSDLNRREFLKGGSFATMMMLMGGIPVNAAEDAADGATS
                     YTGEATPVNVGVIGCGPWARELLKTLSTLPNGPVVALCDNYPAMLRRAGTLAPNAQKY
                     EDYKQLLADKNVQAVVIATPTYLHKDIAIDALKAGKHVYCEAPMASSIEDARAIAKAA
                     QDAFQLNFQVGLQSRADRQIYYLANFIRSGVLGKPLKARQQFHKKQSWRLPSPNPERE
                     KEINWRLDRALSLGLIGELGVHQIDIGSWYLNAQPLSATGFGSLVQWKDGREVPDNVF
                     VVLEYPGGVFLQFEATLGNSFESELSVFYGTDCAVMMRDRRAWMFKEADAPLLGWEVY
                     ARKDAFYNESGIALGAGNTKQEAQKTKDDEPAEVVVDEKSPLQYALGAFLKNSDTLGS
                     GVADFKENFDVSDTAALKEYVGELQKEKSWLPAATWRDGLDSTVTVATANQAILKAER
                     IVFKPEWYELA"
     gene            complement(6046..6723)
                     /locus_tag="PKK20_02670"
     CDS             complement(6046..6723)
                     /locus_tag="PKK20_02670"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU98822.1"
                     /translation="MKDNLMRILTRSMACVALVTLPVSLEAGEATPPSGAVKLSAEEI
                     SQGFRPVFNGVDLKGWHYRNPNGPKSWSVQDGLLVNTLPPGGHGTDLITDEKFKNFTV
                     RYEYKIPPGANSGFYLRGRYEIQILDDGNATRPSNSSNGSLYSLVAPSKMASKKAGEW
                     QTVEATIVDNRITVILNEQKIIDNVVVDRPTGGELDRNLDQPGPIFLQGDHGAVTFRN
                     IRVKPLP"
     gene            6857..7984
                     /locus_tag="PKK20_02675"
     CDS             6857..7984
                     /locus_tag="PKK20_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98823.1"
                     /translation="MCAQGTTLPPRNSFWQRTRFLLALGLILSLTAAISMTSAWRCRQ
                     PAYEGRPLMDWAADLTANDPERVTAAREAVLSLGPRAVPTLIRAIRRPDPILAWPLLE
                     ASARWAPATYGTLIRWLDIDSIRGARRHALVALAILGPGAEPAVPALTRLIRRPTSTR
                     NAVEWRQAAYALGRIGPAAAQPLATVFSEAPVSLHAHLLNALADLGPQAVDAIPTVLS
                     AFERKPDSPWAALAQFLRAAGGDKALAHLYPLLADEDGLLRMRINEVLSQIAETNLEA
                     RNALIEASTTEPGPVRLELLRLLGRLERLHTAVAHRMLRALDDPDQAMRGEGARWLTR
                     RLSPANLDRLLALEPADLQARARQRLGAGPGLDAPADADRR"
     gene            8305..9597
                     /locus_tag="PKK20_02680"
     CDS             8305..9597
                     /locus_tag="PKK20_02680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNU98824.1"
                     /translation="MSAISSTPRELALHGGEPAVRDPLPPWPSFDDNAIQSVEQTLRS
                     GKVNYWTGRKGMEFEKRFAAWQGSAYGISVATGTAALHVGLTALGIGPGDEVIVPSYT
                     FIASSFSIVQAGAIPRFADVNIDDHCLSVESARRLVNERTKAVMPVHLYGNVCDMDRI
                     HAFAKEHRLFVIEDNAEAFGGVYKGKKTGTLGDIAGCSFCQNKTFTTGGEGGMVTTDN
                     EDLAWAARSFRDHGYDVKDRLNLLEMEQRLSYIHNMVGWNYRMTEMQSAIGLAELDRM
                     DTWNMPRRRRNARIIIDALADEPLIKHRPIDTPERQNGWYVMAFSLDIEAMRCDIAEF
                     VKAVGAEGAPCWKVFWPQCHTERAFQDGNAFGRSGFPFKSREYTNPASADYTQVEVPN
                     AVWHQSHTFTCFAFPTYEPHHCEQIGAAIKKVLRAYAK"
     gene            9622..10683
                     /locus_tag="PKK20_02685"
     CDS             9622..10683
                     /locus_tag="PKK20_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98825.1"
                     /translation="MTRTIRWGVIGSTGIARRRTIPEGLATARNAQLVAVYSRQAQTN
                     AEVATQFGAQAAASVDDLLRQDLDAVYIASPPRLHLEQALACARAGKHVLCEKPLGLT
                     VAEAETMVASCRQAQVRLGTAFMMRFLAQHQAALELICTGRIGQPVFARAQLSCWYPP
                     IPGAWRQDPALGGGGSLMDMGGHCIDLLEMLLGPVRAVSCLTSRVVHGYESEDGAVAS
                     LQFACGALGVVDTFFCMPDEASQNRLEVYGSCGSILAAGTIGQGDHGEMVACLQPGAA
                     AYDAQQTRVSNQATPICPAPVNTYRAEIEEFSQAVIEGRDPANSGALGLRSQQMIAAC
                     YESARTGRVVEIPADDPHR"
     gene            complement(10838..11734)
                     /locus_tag="PKK20_02690"
     CDS             complement(10838..11734)
                     /locus_tag="PKK20_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNU98826.1"
                     /translation="MKRSRRSASVVLGLLQTACSAKPDENLQRTVEAATRAARDGAQI
                     LCTQELFRSRYFCQSEDYAHFALAEPIPGPSTKAFQKVARRHRVVVVASLFEKRAPGL
                     HHNTAIVIDADGTLLGRYRKMHIPDDPLYYEKFYFTPGDLGFQAWSTRYGRIGVLICW
                     DQWYPEAARLTALQGAELLFYPTAIGWHPAEKAALGAVQHGAWETIQRAHAVANGCPV
                     AAVNRIGFEKADSGDGIEFWGQSFVAGTSGELLAKASADKEENLLVSVDLAASEVTRT
                     HWPFLRDRRIDAYGGLMRRWGQ"
     gene            12155..13132
                     /locus_tag="PKK20_02695"
     CDS             12155..13132
                     /locus_tag="PKK20_02695"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNU98827.1"
                     /translation="MTATVQVTIHSSQFPDAVRGDLLDSLRQGAINHKFHYDSIKQAQ
                     KWLALHDAYSPARTRPEVAAAYHRAIEQTSRIVSPGTVHVIGLGCGGGAKDCALLSRL
                     QGSAPSYTPIDVSTALVLTSWQAARSIIPPERCFPLVCDLAKADDLPSILDAQPISRA
                     TRLITFFGMLPNFEPEKILPRLAALVRPGDLLLCSANLAPGSNYQEGVARVLPLYDNG
                     LTREWLLVFLSDLGVDSHDGTTTFRIEEHPPGSRLLRIAALFQFVRRTDIRIDSHTFT
                     FQPADGLRLFFSYRHTPELVERLFAAHGLPVSAWWVSSCGEEGVFLVRR"
     gene            13552..>13879
                     /locus_tag="PKK20_02700"
     CDS             13552..>13879
                     /locus_tag="PKK20_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU98828.1"
                     /translation="MRIEFWLLSGVGWRFDRTAARTALTFSSLCLENGSMYWDRQLAE
                     VFQHARRLFPAVLVTGPRQSGKTTFLQHEAADAAYVSFDDPLQRQFATADPNGFLDQF
                     GERPVVL"
BASE COUNT         2466 a         4551 c         4264 g         2598 t
ORIGIN      
        1 ggcgttcacc acgccggtct gctgcccaag tatcgggtgc tggtcgaaca actggctcag
       61 cgcgggctgc tcaagatcat ctgcggcacc gacacgctgg gcgtcggtat caacgtcccc
      121 atccgcaccg tcctgttcac ccgcctgtgc aaatacgacg gccagaagac cggcatcctg
      181 agcgcgcgcg atttccatca gatcagcggg cgggccgggc gcaaaggatt cgacgatcgg
      241 gggtgggtgg tggcccaggc cccggagcac gtcatcgaga acctcaagct cgaggagaaa
      301 tcggcaaaac aggggcgcaa gttcgtcaag cgcaggccac ccgagcgcaa ctttgtcaac
      361 tgggataaga acaccttcac gcgtctgatc ggggcgttgc ccgaacggct gacgtcccgt
      421 ttccaggtgt cgcacggcat gctgctcaat gtgctgagcc ggaacagcga cggctgccgc
      481 gccatgcgcc aactgattcg cgacagccat gaatcgccca aggggaaaag ggcgcatcgg
      541 cgtcgggcgt ggcaactgtt tcgctcgctg cttgaacgca agatcgtcga gttcattccg
      601 cgggccgacc gggccgaggc ggaccatccc gatcgcaagc tgcgcgtgaa cgtggacttg
      661 caggacgatt tctcgatgga ccaggcgctc tccctctatc tgatcgagac cgtcccgaca
      721 ctcgatccgg cggcgcccga ttacgcgctg gacctgctca cgctggtcga gtcgattctc
      781 gaagacccgg acatcatcct gcgcaagcaa ctcgaccggg tgaagtcgca ggcgatggcg
      841 gacatgaagg cccggggact cgagtaccag gagcgcatgg acgaactcga gaaactcgaa
      901 tatcccaaac ccaaccgcga tttcatctac tcgaccttca acgccttcgc caacaagcac
      961 ccgtgggtgg gccaggagaa catccggccc aaatcgatcg cccgcgagat gttcgaacag
     1021 tttcgctcgt tcggcgatta catccgcgat tacgaactcc agcgctccga agggctactg
     1081 ctgcggcatc tgaacagcgt gttcaaggtc ctcgcccaga ctgtccccga cgccgccaag
     1141 acagattcga ttcgcgagat ggaactctac ctgtcggcca tgatccgcca ggtggattcg
     1201 agtctgctcg acgaatggga gaaaatgcgc ggaacgaagc tcgcccaaca atcggagccg
     1261 gcgaccgagg tgcgccctcc tggcgcggac gatgccgcgg ccgacatcac gcggaacacg
     1321 cgcgcattca ccgccgccat ccgaacggtg attttcacct tccttcgagg ttgcgtggtg
     1381 ggcgactacc aagccgcctt ggcggcgttg gccgagccga cggaaggcgc ggccgccgcc
     1441 caaagcccgc aagacacatg gacacccgac cgcctggacc agacgctcga actttacttc
     1501 gccgagcacc aacagatttg cctcgatccg aacgcgcgca acacgcggca cacgtatgtg
     1561 ctgccatcgg aagaccagcg gacgtggcgg gtccagcaga tgctcattga ccccgaggaa
     1621 cacaacgatt gggtggccga gtttgacgtc gatttactca agtcgcgcga actccaggcc
     1681 ccggtcgtgc ggttgcatcg aatcgggctt ctcactcgcc cgtaaccccg caccccctct
     1741 ttgcgccatt gcgtcttcgc ccctctgcgt tccctccggg ccgagccgac ttcaatgcaa
     1801 agacgccaag acgcagagac gcagagcaag accggtagcg cctttcatct cccgcaaccc
     1861 tcatcgaccc cacacctggc ggtcgaggct gcggagctgg atggcctcgg ccagatggtc
     1921 ggggacgatc cggtcggagt cggcaaggtc ggcgatggtc cggctcactt tgaggatgcg
     1981 gtcgtaggcg cgcgcgctca ggtcgaggtc ggtcatggcg accttgagca tttcgagcgt
     2041 cgtttcatcg agggcgcacc accgtttgag atcgcggctg gtcatgcggg cgttgcatgg
     2101 gacgcgcgag cgtccggcga atcgctcctg ctggcgtttt cgggcggcga cgaccctttc
     2161 gcggacgcac gccgacggtt cccccatgcc ttcggccgtc atatcgcgaa acttcacggg
     2221 cggcacctcg acatggagat cgatgcgatc gagcagcggc ccggaaatcc gtcccaggta
     2281 gttctggatc tcccgcggcg agcagcgcga ttcgcccggc atcttgccgt caggggttgg
     2341 gttcatggcg gcgacgagca tgaacgccgc cggaaaggtc atcgtgccgg cggcgcgcga
     2401 gatggtgacg cggccctctt cgagcggctg gcgcatggtc tcgaggacac tgcgtttgaa
     2461 ctcgggcagt tcatcgagga acagaacgcc attgtgcgcc aacgagattt cgcccggcgt
     2521 gggcacgatg ttgccgccca gcaagccggc gtcgctggcc gtgtgatgcg gcgtccggaa
     2581 gggccggcgt gtgaccaggg cctggccgtt gagctggccg acgatgctgt ggatcttcgt
     2641 tgtctcgagc gcttcttcga gcgtcatggg cggcaggatc gtcggaaggc gcttggcaag
     2701 catcgatttg ccggtgcccg gcggtccgat gagcagcacg ttgtgtccgc ccgcggccgc
     2761 gatctcgagc gcgcgcttca ccgattcctg cccttttaca tcggcgaaat ccagttcgtc
     2821 ctcgatcggc tgtgcgaaga gccgagccat gtcgaggcgg acgggggcga gcgtgatttc
     2881 gccttcgagg aaagcgacgg cctcacgcag gttctggacg ggaatcacgt tgaggcccgc
     2941 gaccacggcg gcctccgcgg cgttgtccgg aggcacaagc agcccggtct tcccgtcggc
     3001 cttggcgcgc agcgcgatcg gcaggacgcc cttgaccggg cgaacggcgc cggtgagggc
     3061 cagttcgccg atgatgagga aattgtccaa ctgatcgagg agcatctgct cgctggcggc
     3121 gatcatgccg actgcgattg ggagatcgaa gctgggaccc tcctttttca cgtccgccgg
     3181 cgcgaggttg atcgtcatgc ggcccagcgg gaatcgaaaa ccggagttgc tgagggcggt
     3241 ggtgacgcga tcgcgcgatt ctttgacggc ggcatcgggc aggccaacga tgacgatggt
     3301 cgtatcgccc cagccggagt tcacctcgac ttcgacggca taggcctcga ttccattaac
     3361 ggctgccgaa cccaccttgg ccaacatggc actactagtg gcccagcaaa cggcgatgcg
     3421 caaccggttt ttgtgaggcc ggcttttgcg aagcgtgtgt taggatcgga acgccccgat
     3481 gaaaagcgca aagcgcggcc ggtcgggccg cgcttgcgca aaggaatatc cgacgatcgc
     3541 gtcagcccgc tatttcgcgg cggcgtcggt tttctgcagg gcgaggagcc actcgaattt
     3601 gatcgtgact tcgtcgccgg ttttgatcgg gctcatgccc aggatgttgg gggccggggg
     3661 cggcaccttg tagtccgtca tcttgagaac cttcgatccg gagaatttga ttcgatcgtc
     3721 ggcgacgcgg gtcatggtga cctccatatc gatcgtgttg gtgacgccgg agattgtcag
     3781 atcgcctttg gtgtcgaact tgaccgggct gcctgaggcc ggcacgtcgc ctttgacgat
     3841 catttccttc agcgtgtaga ggatgtccgg gttgtccttc atgcgcatgg cttcctgcat
     3901 gacttcatcc atcttcgatg cgccgagcag gacctggctc ttgagggact tgacggggat
     3961 tttgacctgg accttgggat tgacctcttt ggtcgtgagg ctcttgaccg acttgagcgt
     4021 cttgtcggtg aggaactcgg gctcgacctc gaacgaaccg ccgatgatcg cgcctttggt
     4081 tgtccagtcg tgaatggtcg atgtgccgtc gatgctcacc aggcagccgg gtctggctgc
     4141 gtatttgatc aagtcgtcgg cggcctgggc gagcatggcg caggccaggg cgacggcgag
     4201 tgtcagagtc gatttcagca gatgcatttt catatttgtt gttttggtct tagatgggag
     4261 atgaaacggg aaaattcaga ttggctgtgt tttctaaccc gcatagcagt cctcttgcga
     4321 agtctgactg ccatatgcac agacggggtg cgaaatatgc gggttaggcc aattcgtacc
     4381 actcgggttt gaaaacgatg cgttcggcct tgaggatggc ctggttggcg gtcgccaccg
     4441 tgacggtcga gtcgaggccg tcgcgccatg tggccgccgg cagccagctc ttctcctttt
     4501 gcagttcccc gacgtactcc ttgagagccg ccgtgtcgct gacatcgaag ttctccttga
     4561 agtcggccac gcccgaaccg agcgtgtcgc tgttcttgag gaatgcgccc aacgcgtatt
     4621 gcagcggcga tttctcgtcg accacaactt ccgcgggctc gtcgtctttg gttttctggg
     4681 cctcctgttt ggtgttgccg gcgcccagag cgatgccgct ttcgttgtag aaggcgtcct
     4741 tgcgggcgta aacctcccag cccagaagcg gggcgtccgc ctctttgaac atccaggcgc
     4801 ggcggtcgcg catcatgacc gcgcagtcgg tgccgtagaa cacactcaac tccgactcga
     4861 acgaattgcc gagtgtcgcc tcgaattgga gaaagacacc gcccggatac tcgagcacca
     4921 caaacacgtt gtccggcacc tcgcgtccgt ccttccactg caccagcgag ccgaagccgg
     4981 tggccgagag cggttgcgcg ttcaagtacc aactgccgat gtcgatctgg tgcacgccca
     5041 gctcgccgat cagtccgagc gagagggccc ggtccagacg ccagttgatc tccttctcgc
     5101 gctccgggtt gggcgatgga aggcgccagc tttgcttctt gtgaaactgc tggcgggcct
     5161 taagcggttt gcccaggacg cctgagcgaa tgaaattggc aagatagtag atttgcctgt
     5221 cggcccggct ctgcaggccg acctgaaaat tgagttggaa ggcgtcctgc gcagccttcg
     5281 cgatggcgcg ggcatcctcg atgctgctgg ccataggcgc ctcgcagtag acgtgtttgc
     5341 cggccttcag ggcatcgatt gcaatgtcct tgtgcagata cgttggcgtg gcaatcacca
     5401 ccgcctggac attcttgtcg gccagcagtt gcttgtagtc ctcgtacttc tgcgcgttgg
     5461 gcgccaacgt cccggcgcgc cgcagcatcg cgggatagtt gtcgcaaagg gcgacgaccg
     5521 gcccgttggg aagagtggac agggtcttga gtaactcgcg cgcccacgga ccgcagccga
     5581 tcacgcccac attcaccggc gtggcttcgc ccgtgtagct ggtcgccccg tccgcggcgt
     5641 cctccgccgc gttgaccggg atgccgccca tcagcatcat catggtcgcg aacgacccgc
     5701 ctttgaggaa ttccctacga ttcagatcgg acattctgtc gcttttcatg gtttacagac
     5761 cgattggcat tcttggccgg ggccatcaca cagaatccag tcgatggccg cctgcagcat
     5821 gcgttcagtt tctcccgccg ccgcctcggt tggcaaacgg aatcttcagc ccactcccgt
     5881 ccggtgcgtc tggcaggtgt cggtattctg tcgcccagag aggacaccgg gcctacaccg
     5941 gatggagacc tccgctgcag gccgcgtagg ggcacgcggc gcaaactcca ttcccatcgc
     6001 caactcacaa atgcactgtc cccattctca gcctcgtttc ccgcatcaag gaagcggttt
     6061 cacgcgaatg ttgcggaagg tgaccgcccc atgatctccc tgaaggaaga tcgggccggg
     6121 ctgatcgaga ttgcgatcga gctcgccgcc cgtcgggcgg tccacgacga cgttgtcgat
     6181 gattttctgc tcgttgagga tgaccgtgat ccggttgtcg acgatcgtcg cctcgaccgt
     6241 ctgccattca ccggccttct tcgacgccat cttcgagggc gccaccaggc tgtagagcga
     6301 gccgttgctg ctgttggatg gccgggtcgc attcccatcg tcgagaatct gaatctcgta
     6361 gcgcccgcgc agatagaacc cgctgttggc gccgggcgga atcttgtact cgtaccggac
     6421 ggtgaagttc ttaaacttct catccgtaat caaatcggtc ccgtgcccgc cgggcggcaa
     6481 ggtgttcacc agcaggccat cctggacgct ccaactcttg gggccgttgg ggttgcgata
     6541 gtgccagcct ttcaggtcga ccccgttgaa aacaggacgg aatccctgac tgatctcttc
     6601 agccgaaagc ttcacagcgc cgctgggcgg cgtggcctcg ccggcttcga gcgagacagg
     6661 cagagtgaca agggccacgc acgccatact ccgggtgaga atccgcatca gattgtcctt
     6721 catgtgtttg tgtgtttggt gttgccgcaa gtgcacgcga tatggacgtt ccgatgcctc
     6781 catgcattca tgcacagact gagaggagtc tgaaagaacc tcgcattcca ggccgccttg
     6841 aacgatgatg ctcgctatgt gcgcgcaagg gacaacgctg ccgccgcgaa actcgttctg
     6901 gcaacgaacc cgtttcctgc tggcgctggg gctgattctg agcctgacgg ctgcaatctc
     6961 catgacctcg gcgtggcgat gccggcaacc cgcctacgag ggacggccgc tcatggattg
     7021 ggctgccgac ctgaccgcca acgaccctga gcgggtcacg gctgcgcgag aggccgtctt
     7081 atcgctcggt ccgcgcgcgg tgccgacgtt gatccgtgca atccgccgac cggaccctat
     7141 tctggcctgg cccttgctcg aagcgagcgc gcgctgggcc cccgccacat acggaaccct
     7201 catccgctgg ctggatatcg actcgatccg cggcgcgcgg cgtcatgccc tggtagccct
     7261 cgcgattctc ggccccgggg cggagccggc cgtgcccgca ttgacgcgct tgatccgcag
     7321 gccgacttcg actcggaacg cggtagaatg gaggcaggcg gcttatgcgc ttgggcggat
     7381 tggccctgca gccgctcagc cattggccac ggtgttttcc gaagctcccg tttcactgca
     7441 cgcgcatctc ttgaacgccc tcgcagacct cgggcctcag gccgtcgatg ccatcccgac
     7501 cgtcctgtcg gcgttcgaac gaaaaccgga ttcgccgtgg gccgccctgg cgcagttcct
     7561 ccgggcggca gggggcgaca aggccctcgc gcatctctat ccgctgctcg cggacgagga
     7621 cggcctgctg cgaatgcgaa tcaacgaggt cctgtctcaa atcgccgaaa ccaatctcga
     7681 ggcccggaac gctttgatcg aagccagcac gaccgaaccg ggcccggtgc ggctcgagtt
     7741 gctcagactg ctggggcggc tcgaacgtct ccacaccgcg gtagcccacc gcatgctgcg
     7801 cgcgctcgac gatccggacc aggcgatgcg cggggaaggg gcacgctggc tcacccgccg
     7861 cctttcgccg gccaacctcg atcggctcct ggccctcgaa ccggccgacc tccaggcccg
     7921 ggcacggcaa agactcggcg ccggacccgg attggatgcg cctgccgacg ccgatcgccg
     7981 atgaacggca gcgcgcgaca gcgcggcgaa gccgcagcca cagcccggac gccaacattg
     8041 aatcacacag tgcatctgga ggttgtcggt ggaaatcgag tcggcgccgc gtggggcctg
     8101 cctgccgcgc ctgcctgcgc cgtgcccgac acggcagaca ggccgctgcg ctttgcggct
     8161 caggcaggac acgcggtcta caccggcagt ctccatcggt tgtaggcccg ctgccctcac
     8221 cgggcgacac tacaccgacg acaaccaggt gcaccgcatc gaatcgtgtt gccgaaacgc
     8281 ggcgcaggct gttcaatgcg tcccatgtca gccatatcct cgacgccccg cgaactcgcc
     8341 ctgcacggcg gtgagcccgc cgttcgggac ccgctcccgc cctggccctc ttttgacgac
     8401 aacgcgatcc agtcggtcga gcagaccctg cgctccggga aagtgaacta ctggacgggg
     8461 cgcaagggga tggagttcga gaagcgtttt gcggcctggc agggcagcgc ctacgggatc
     8521 agcgtggcca ccggcaccgc tgcgctgcac gtcggcctca cggccctcgg gatcgggccg
     8581 ggcgacgagg tgattgtgcc cagctacacc ttcattgcat cgagtttctc gatcgtccag
     8641 gccggcgcca ttccgcggtt cgccgatgtg aacatcgatg accattgtct gagcgtggaa
     8701 tcggcccggc ggctggtcaa cgaacggacg aaggcggtga tgccggtgca tctctacggc
     8761 aacgtgtgcg acatggaccg gatccacgcc ttcgccaagg aacatcggct cttcgtcatc
     8821 gaagacaacg ccgaggcgtt cggcggcgtc tacaagggaa agaagaccgg cacgctcggc
     8881 gacatcgccg gctgcagttt ctgccagaac aagaccttca ccacaggcgg cgagggcggc
     8941 atggtcacca cagacaacga agacctcgcc tgggccgccc gcagttttcg cgaccatggc
     9001 tacgacgtca aagaccggct caaccttctc gaaatggaac agaggctctc ctatatccac
     9061 aacatggtcg gctggaatta ccgcatgacc gagatgcagt ccgccatcgg cctggccgaa
     9121 ctcgatcgca tggacacctg gaacatgcct cgccgccgcc gcaacgcccg gatcatcatc
     9181 gacgccctcg ccgacgaacc gctcatcaag catcgcccca tcgacacccc cgaacggcag
     9241 aacggctggt atgtcatggc cttctcgctc gatatcgagg ccatgcgatg cgatatcgcg
     9301 gagttcgtca aagcggtcgg cgccgaagga gcgccctgtt ggaaggtttt ctggccgcaa
     9361 tgccacaccg aacgcgcctt tcaggacggc aatgccttcg gccgctcggg cttccctttc
     9421 aaatccaggg agtacaccaa tccggccagt gcggactaca cacaggtcga ggtgcccaat
     9481 gccgtctggc atcagagcca taccttcacc tgcttcgcgt tcccgaccta cgagccgcac
     9541 cactgcgagc agatcggcgc cgccatcaaa aaggtcctcc gcgcctatgc gaagtgactc
     9601 catccaatcg agggcaatcc aatgacccgc acaatccgct ggggcgtcat cggctcgacg
     9661 ggcatcgccc ggcgacgcac gatccccgaa ggccttgcga ccgcgcgcaa cgcgcaactc
     9721 gtggccgtgt acagtcgcca ggcccaaacc aatgccgagg tggcgaccca gttcggcgcg
     9781 caagccgccg ccagcgtgga cgatctcctg cgtcaggacc tcgatgccgt ctacatcgca
     9841 tcgccgccac gccttcacct cgaacaggcg ctcgcctgcg cccgggccgg caagcacgtc
     9901 ctctgcgaga aaccgctcgg cctgaccgtc gccgaagccg agaccatggt cgcgtcgtgc
     9961 cgccaggcac aggttcggct cggcacggct ttcatgatgc gttttctcgc ccagcaccag
    10021 gcggccctcg aactcatttg cacaggccga atcggccagc cagtcttcgc ccgcgcccag
    10081 ctctcgtgct ggtatccgcc catccccggc gcgtggcgcc aggatccagc cctgggcggg
    10141 ggagggtcgc tcatggacat gggcggacac tgcatcgacc tgctcgagat gctgctcgga
    10201 cccgtccgcg ccgtaagctg cctcaccagc cgcgtcgttc atgggtacga gtccgaagac
    10261 ggcgccgtcg cctcgctcca gttcgcgtgc ggggcgctgg gcgttgtgga cactttcttc
    10321 tgcatgccgg acgaggccag ccagaaccgc ctcgaggtgt atggctcgtg cgggagcatt
    10381 ctcgccgccg gcaccatcgg gcagggcgac cacggggaaa tggtggcctg cttgcagcca
    10441 ggcgcggcgg cctacgacgc acagcagacc cgcgtgagca accaggccac gccaatctgt
    10501 ccagcacccg tcaacaccta ccgcgccgaa atcgaggagt tcagccaggc cgttatcgaa
    10561 gggcgcgacc cggccaacag cggcgccctt ggcctgcgca gccaacagat gatcgccgcc
    10621 tgttatgagt ccgcccgtac cggacgtgtt gtcgagattc ccgcggacga tccgcatcgg
    10681 tgacttccgc gagaccctgg ctcgatacaa tcaccgtcat cggtctgggc ctcccctgcc
    10741 accgctggat cggccctccg agagtggcgg tccatggaga actgacatcc aacagtccaa
    10801 tccgcaccag gaccatcaac cactctcaac ccgcagatca ctgcccccac cgtcgcatca
    10861 gcccgccgta agcgtcgatg cgccggtcgc gcagaaatgg ccaatgcgtg cgggtcacct
    10921 ccgacgccgc aaggtcgacg ctcaccagga ggttctcctc cttgtccgcg ctcgccttgg
    10981 ccaggagttc gcccgaagtc cccgccacga agctctgccc ccagaactcg attccatcac
    11041 cgctatcggc cttttcgaag ccgatgcgat tgaccgcagc caccgggcag ccgttggcca
    11101 ccgcgtgggc gcgttggatg gtctcccagg ccccgtgttg cacggcgcca agcgcggcct
    11161 tctcggccgg atgccagccg atggccgtcg ggtagaacaa caactccgcc ccttgcagcg
    11221 ccgtgagccg ggcggcttcg ggataccact gatcccaaca gatgaggacg ccgatgcggc
    11281 catagcgcgt ggaccatgcc tggaaaccca ggtcgccggg cgtgaaatag aacttctcgt
    11341 aataaagcgg gtcgtccggg atgtgcatct tccggtagcg acccagcaga gtgccgtccg
    11401 cgtcgatcac aatcgcggtg ttgtgatgca agcccggggc gcgcttctcg aacagcgagg
    11461 cgaccaccac cacgcgatgg cgccgcgcaa ctttctggaa agccttcgtc gagggaccgg
    11521 ggatcggctc ggccagggcg aagtgggcgt agtcctcgct ctgacagaaa tagcgcgagc
    11581 ggaacagctc ttgcgtgcaa agaatctgcg cgccgtcgcg cgccgcccgc gtcgccgcct
    11641 cgaccgtgcg ctgcaggttc tcgtccggct tcgccgaaca cgcggtctgc agcagtccca
    11701 aaaccaccga tgccgagcgc ctcgaacgtt tcatggattc gagtgtattg cgtcagacgg
    11761 caaagtccaa ctcccccgtc gggcaatcga tcctcttcgg tgcatttggt tgttggcggt
    11821 cttctgtcgc ccggtgatgg catcgggcct acagcagatg gagactcccg ctgtaggccg
    11881 cgcgccctgc ctgtgccgca aagcgcagca gcctgtctgc cgtatcgggc acggcgcagg
    11941 caggcgcggc aggcaggccc cacggggcgc aaactctatt cccgccacag gatgaatggg
    12001 ccaaggcgca tccgatcttt gcagggctgc caaccgggat gctcgattac acatactacc
    12061 gcgagatcaa tcagccgctg gcggacctga ggcccaactt cgaggatgaa atgcgtgcgt
    12121 gcggcctttt ctagcctgta tctcgaacca ctctatgacc gcaaccgttc aagtcacgat
    12181 ccattccagc cagtttccgg atgcggtgcg cggcgacctc ctggactccc tgcgtcaagg
    12241 cgccatcaac cacaagttcc attacgacag catcaagcag gcccagaaat ggttggcgct
    12301 gcacgacgcc tactcgccgg cacgcacacg ccccgaagtc gccgccgcct accaccgtgc
    12361 gatcgagcag acttcgagaa tcgtcagccc cggcacggtg cacgtgattg gcctgggttg
    12421 tggcggcggc gcgaaggact gcgccctgct cagccgcctc caaggaagcg caccgtctta
    12481 caccccgatc gacgtcagca ccgctctcgt cctgacatcc tggcaggccg cccgctccat
    12541 catcccccct gaacgctgct ttcccctcgt gtgcgatctg gcaaaggcgg atgatctgcc
    12601 gtccatcctg gatgcacagc ccatctcccg cgccacgcgt cttatcacct tcttcggcat
    12661 gctgcccaat ttcgagcccg agaagattct tcccagactg gcggccttgg ttcggcctgg
    12721 cgatctcctg ctctgcagcg ccaatctcgc gccaggctcc aactatcaag agggggtggc
    12781 ccgggtactg ccgctctacg acaacgggct gacgcgagag tggcttctgg tgttcctgtc
    12841 ggacctgggt gtcgactcac acgacggaac gacgaccttt cgaatcgaag agcatcctcc
    12901 cggatcgcgg ctgctgcgca tcgccgcctt gttccaattc gtgcggcgca ccgatatccg
    12961 catcgactcg cacacattca cctttcaacc ggccgatggg ctgcgtctat tcttttcgta
    13021 tcggcacacc cccgagttgg tggaacgttt gttcgccgcc catggcttgc ctgtctcggc
    13081 ctggtgggtc tcgtcctgcg gcgaggaagg ggtcttcctt gtgcgtcgtt aaaaagcatg
    13141 cggtgtatcc gggcttcccg cgccgcgatt ggccttggca cggctgcact gtggcgcgca
    13201 cggtgtggcc atcctcaatc aggctgcccg gccgttcagc atctcgccat gcaggtgagt
    13261 gccacatcga ctccggcaaa cgaactcctg gccttcggtc agaagcacgc cacccgccaa
    13321 gtcacgctcc tgttcgctga cctggttgac tccgttgccc tcaagcaacg tctgggcagg
    13381 cgcagtgcgt gttcatgggt gacttgattg gttgagttcg ggaagacaga aggcggcttc
    13441 tcgtcggttc atgacctcgc agcatggact tcaggccctc cgccggtgcc tctcccgcct
    13501 tgaccgacgg gagagagctg cgtccgtggc cgggggcggg gcgagaagaa gatgaggatc
    13561 gagttttggc ttttgtctgg cgttggttgg cgtttcgatc gaaccgcagc gagaactgcc
    13621 ttgacatttt caagtctgtg tcttgaaaat ggcagcatgt actgggatcg tcaactggcc
    13681 gaagtcttcc aacacgcccg tcgcctgttc cccgccgtgc tcgttaccgg ccctcgccaa
    13741 tcgggtaaaa caaccttcct ccaacacgag gcggcggacg ctgcgtatgt ctctttcgac
    13801 gacccgctgc agcgtcagtt cgccaccgcc gatccgaacg gctttctcga ccagttcggc
    13861 gagcgcccgg tggtgctcg
//
LOCUS       DAOQRO010000040        13530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3612_length_13530_cov_7.304267, whole genome shotgun sequence.
ACCESSION   DAOQRO010000040 DAOQRO010000000
VERSION     DAOQRO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_13530_cov_7.304267"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKK20_02705"
     CDS             <1..453
                     /locus_tag="PKK20_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98829.1"
                     /translation="RGNTNDLKTFGHQVHKIKKELGCTGVTLVGDRGMIRTDQKAACT
                     ADNTQAHALTAMLALKVRRHLEQAWRSLEVTVEEGLRQLEQLCVMELVHSGSGKVVAR
                     QVPEPSARQQQLLQALKLVLPATVPEAQVTVGTRKKNKAENHDSAIRS"
     gene            complement(672..1637)
                     /locus_tag="PKK20_02710"
     CDS             complement(672..1637)
                     /locus_tag="PKK20_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNU98830.1"
                     /translation="MPLTHTKELFAKALKGKYALGAFNVNNMELVQAIVEACEEEKAP
                     LILQISKGARQYANPVYLKKLIEAAVSVSNIPIAVHLDHGDTFELCKQCIDEGFTSVM
                     IDGSHLPFDKNAELCKRVVDYAHKHNCVVEGELGQLVGAQHDDGEEGGGYSKGGVYTH
                     PDEAVEFVKQTKVDSLAVAIGNSHGAYKFKGTQHLNLERLKAIKKALLDAGLGDYPLV
                     LHGASSVPKALVQEINQYGGKLGEDAAGVPEADIEIARRTGCTKVNIDTDLRLALTAG
                     IRKAFAENPKEFDPRKYLGPARAKVKDLVRHKVKNVLCCAGHAFD"
     gene            complement(1872..9110)
                     /locus_tag="PKK20_02715"
     CDS             complement(1872..9110)
                     /locus_tag="PKK20_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98831.1"
                     /translation="MHRFLGPRRGKDTTESARFSHILKERRDALCPPLDRHRNHGGAL
                     VHPIAAQSIVASALLALWLAASTFAGDPDPAPAIVPPPTPASEPAATSPTAVEVNAIA
                     LDGQLGAEKARLVIEADLRGLSSGREKCIPSAAIQQTIAVSRAKLTHTISIQIDAVQG
                     ELKEVELWLSGVGEITRVTSDALEHWSVRRGPDGARSLVVRVKRADKPVKSLVVQAVA
                     ETSLGDLPQTVTPLALIGDPASLSHGYVRIDTPPELAIQVGNPAGVAPIESKFLPESL
                     RPVVTPGAPETIAFRFHGTPYSLPLTIEAADPESSRVVVSQFRLQGRLSGDSAEFTLS
                     GLARVKNPKGGSLTLLAGRAALTEVNAAPHWHWRLEGNKYVAVFDRDGEFALRLKFNA
                     AVRQTNGWNELDFVVAPGGLAPVTIEGLKADTEFRFADGARPERAGEEFVSFLPPTGQ
                     VQLAWKEARPETEGALFYAAEALAQIAISPGLMRQTALLDFKVMQGELNRVTLRLRGA
                     GEVARVQGPAVLSWVVEPLPNSADRRLAVQLNEAQKDQFSFQVQLQTPLGAFPQAVEA
                     MQVLPEGTTRFGGCYRIVNEGAVRLEVLQASGLSQISPEQFPQTDATQTLALTEQTTQ
                     VFAYRFSGGAFELRMQADNILPELAVSALIEYHLGETELAIDAEFEVDVREAPLREML
                     LRIPKGYAVARLNAPGLGDYFPTESADLPEAQLRLVYGAPVSGRQVVQLRLERNQPLA
                     ESAWRLPRIDVLKAKSVRGHVGVTADAGFRLTPGATQGLTEIAGAFFPKKLTGIQAAF
                     RMSDAAWQATLAVERLPQSIQADTFHLFSIGEGVAYGSSIINYVVSGAPIAVFKIELS
                     AEYFNVEFSGKDVRSWQKTDGGYQVQLHTPVAGAYTLLATYERPFKPQGDTLTFTGAR
                     PLDAQSEQGHTIVVSAYQFQVKPVNLSNALLPLETGEVPAEYRLFFDAPILAAYRYAA
                     RPFNLQLELKPLAQSETISQVVDRASFVTRISDEGEVVTDARYFVKNKGAPHLRLVIP
                     EGTQLWSVTVNNAPVVPVTSDRAHLIPLPQHADADAVNDLQIKLAARAKDRKRIAVAA
                     PIVSAPVLLAEWHLVPDTGRRLVCRRGPLTPVGATESVTGFAGLQRLLSSEARDDLLL
                     GLGLALGLTIIAAWLWRLASDQGVHKLTLRHLLGGILALAAAALAAIALADLVDLARS
                     ARVEVPRDLRFVAPVQQADSALALDIANVSVGPSWFASAWALAPALPGVLLWLYGLAS
                     SKAWLRVLARVLGWVLMLWAALRWPNGAPLFFGVLLAFVVLHVFVPWLWRWWRAPRKP
                     APDTGSAAAAAALLMFGAWLLESAATLAQADQATGPVPNKTPPLAESVVQEVRVEGQY
                     AIGSARVRWQARQGDVLPLLHAPGVLTRIEYPTNSARLVQTAAADWRDTALLAEQTGL
                     CEIRLDYQVQTVTREGERAFALPTAHGLVNRLTLTLVGLDVEINAPQAVSVQSVASGA
                     STNTVARLVLAPARAPWIAWKPRSRDTRLEKAVFYAELSQLFVPGAGVVEALHQVQLR
                     PAQGELSELEFDVPAGATITDVVAPGLSLWRFDPQSRQLRVRLTPAQSKPFNLLVKSQ
                     IATGPLPFEQTAGLLGVRGAAGQLGLLGVATGNEVQLDDVRAESFSPINLEDFPAAML
                     DPLRPAIAGLTLRRAFRYSDAAANLTLEASAVQPDIRVEAEQTLSLGEDRTVLAANIE
                     VEVTRAGIFKLSFLLPTGLDVESISGPALSHWTELKTDAGRAITLHLKSKTEGKQTFA
                     VSLSGPGVRSATGWAVPRLGVREAGKQRGQLLLVPEQGLRLQIASREGVTQLDPLKAG
                     VRQKGVLAFRLLQSDWSLTLDLERMDAWTQVASLQHVVIGDAQLKVAVNLQYEIENTG
                     IKTLGVRLPADAEGVRFRGEQLTDFRPLEGQAGAGTRDWEIKLNRRVFGKYLLQVTYN
                     LPLGDRLAETGIVGVEALDANMQRGFLTVQAGGRLQVRVDSPPEALQPAEWQSIPRAL
                     QQDVAATAANYTFRLVEPAYRLPVQIERHAAAKLLPARVDSLTLSSVVSDDGIVLTQA
                     RIQLTPGDKRLLHLTLPDKARFWFAFVNQSSVWPWRETNQVLIPLEQHAKTGQPTLVE
                     FFYAARAGAGSSRALNLELLGPRFDLPLENISWRVFLGEQWRLSSWRGSLQLQPEQGP
                     TDGVAVDLETYIRNEAALQQAKTKEAEQFLNWANRLLVEGDPEQARRAFTTAYGLSQH
                     DNAFNEDARVQLHNLKMQQALVGLNVRQAKVAGEPSVLAATPRALRESQAANYTQQEA
                     KQLIERNTAEDNAVQMRLAERLIRQQDAAAASPAAIRVTIPEQGRLLTFSRPLHVQPW
                     ADLALDINARPAQSTTLLFRLTILVCVVLLVGFFAWLGRTKTPPRASE"
     gene            complement(9389..13009)
                     /locus_tag="PKK20_02720"
     CDS             complement(9389..13009)
                     /locus_tag="PKK20_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNU98832.1"
                     /translation="MNPIKPSLPPDDFESKITALLLGELAPDEARAVEQAIAADTTLR
                     ALRDRLRTILPLVRQAVGGPDASAESCANGPRLSAERRDSLLRHFKTIVPRGFDLPRS
                     RRHSWLVPLSAAAVLIVMASLLLPSLAKAKSKGRSIAYRHDAPQVALVTRLGSQPADE
                     PADEPADVLLDAQSQIDPVTGMPMRGESEGATEGRERQLQTYQPGGAPRLKGVEVAAK
                     AESSGAESAQPMADIRMLMRYGLLPKGMKLAEVAGESTAASGLAKAVEKRKTDAISLG
                     ESVAMQPQPEASTPVPSAPARSELAPLPLELPAPAFVGTPTADLAFADGEAKNRAAEI
                     RGGEESAGAMTWGETVAKYGDIQQNWHFDAAAEGTLVNGFDTAEWGRSGGGGMGGMGM
                     AGGMGGVGGAGVSGGFGGGVQTDGDRSSTDFYLGRQVATDRGQDPATHLFSTIRAKAG
                     EPDSDSAGTTEVAQRLSKSDHAGVRITSRSTPAPDRGFLVAGADSLASLAQAGRPTRR
                     AAAPAAASAAAPVATPAPPTMPSLVAEVPPSGGVAGPASSSDAKANAERYGAGYYAYG
                     LPAQEAIEVAKAPVLGDAPMLGAAFRSLEVEPERLERVTAAANAPATEPEPVGLLTPS
                     AGKSVDSSVRRGALVSGEQRGRARFAKSSSAPTRQLADKEQAVDRLAESEKQVADLRL
                     GVAVVNGEGQGLQPQALDVAASVVAAPPVAAPDSGVDALGLMTNVAVSAEALEANGRV
                     AGDVVVKGVPRAASRPSAPPPEPQPEVATAVNPFSTFSLNVTDVSFKLAAASLEQGAL
                     PVASRIRSEEFLNAFNYRDPEPVRGAPVAFAWERARYPFAHNREAIRFSVQTAARGRS
                     ALRPLNLVLLLDSSGSMERADRVAIVREALSVLAQKLEAQDRISVVAFARTARLWVDG
                     VSGSQAREVLDGISRLTPEGGTNLEDALNVAYETAARHFVSHGVNRVVLLTDGAANLG
                     DVDPESLKQKVESARKKGIALDCFGIGWEGYNDDLLEVLSSHGDGRYGFVNSPEEAAT
                     GLADQLAGALEVAAADVKVQVEFNPQRATAWRQIGYAKHQLTKEQFRDNTVDAAELGA
                     AESGNALYILELNPRGQGDVGTVRVRYREAASGLYREHAWRVPYTGPAPGLEESSPAM
                     RLAVTSAAFAEWLASSPFAGEVTPDKLLGAMAGVTDAFGPDARPRQLETMLRAAKAIS
                     GQ"
     gene            complement(13006..>13530)
                     /locus_tag="PKK20_02725"
     CDS             complement(13006..>13530)
                     /locus_tag="PKK20_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNU98833.1"
                     /translation="RPESVEDLFEALESPLLRYALRLVPDADTAEDVVQESFMRLHTH
                     FAEVREPRGWLYQTTHNLALNHQRKTARITLLDVARDRSGGSFDPSNDPPDPQPHPDE
                     QTARREGIGLVRSGLKSLDARARQVVELKFHEDLSYKEISARTGLTVGHVGYILHHAL
                     KSLAAELERAGFVP"
BASE COUNT         2273 a         4386 c         4531 g         2340 t
ORIGIN      
        1 cgcggcaaca ccaacgatct caaaaccttc ggccaccaag tccataagat caaaaaggag
       61 cttggctgca cgggcgtcac cctggtgggc gaccggggca tgattcgcac cgaccagaaa
      121 gcggcatgca cggcagacaa cacccaagcc cacgctttga cggcgatgct ggccttaaaa
      181 gtgcgccgtc atttggagca agcctggcgg tcgttggaag tcacggtgga agaaggcttg
      241 cgccaactcg aacagctctg cgttatggaa ctggttcact cgggcagcgg caaggtcgtg
      301 gcgcgccagg tgcctgaacc cagtgcccgg cagcagcaac tgctccaggc actgaagttg
      361 gttcttccgg cgacggtgcc cgaagcccaa gtcaccgtag gcacgcgcaa gaaaaataag
      421 gccgaaaacc acgattccgc catcagatct taatggaact tccggtagaa agcggcagac
      481 gcatcctcgg gtgaggatgc gtctggcgcg ggtatgattg aatcgggacc ggaggttctt
      541 cgggccggtc gttgtcgtgg actggaaccg agaactgccg aggcggcggt ccgggaagag
      601 ggtgcgccgc ctcgatctga accggatgtc tctcgaaact ccgcgcagag tctcacatcc
      661 gggctccgcg atcagtcgaa cgcgtgaccg gcgcaacaga gcacgttctt gaccttgtgc
      721 cgaaccaggt ctttgacctt ggcgcgggca ggcccgaggt acttgcgcgg atcgaattcc
      781 ttcgggtttt cggcgaaggc cttgcggatg ccggcggtca gggccaggcg caggtcggtg
      841 tcgatattca ccttggtgca gccagtcctc cgggcgatct cgatgtcggc ctcgggcaca
      901 ccggcggcgt cttcgcccag tttgccgccg tactggttga tttcctggac gagggctttg
      961 ggcacgctcg atgcgccgtg gagcaccagc ggataatcgc ccagaccggc atccaggagc
     1021 gccttcttga tggccttgag acgctcgagg ttcagatgct gcgtcccttt gaacttgtat
     1081 gcgccatgcg agttgccgat ggccacagcc agcgagtcga cctttgtttg cttcacgaac
     1141 tcgactgctt catcggggtg cgtatagacg ccgcccttcg agtagccgcc gccctcctca
     1201 ccgtcatcgt gttgcgcgcc aaccagttgg cctagctcgc cctcgacaac gcagttgtgc
     1261 ttgtgcgcgt aatcgaccac ccgtttgcag agttcggcgt tcttgtcgaa tggcaagtgg
     1321 ctgccgtcga tcatcacgct ggtgaatccc tcgtcgatgc attgcttgca cagttcgaag
     1381 gtgtcgccgt gatcgaggtg gaccgcgatg gggatgttgc tcacgctcac ggccgcttcg
     1441 atgagcttct tcaggtagac agggttggca tattgccgcg cacccttgga aatctgcagg
     1501 ataaggggag ctttctcttc ctcgcaggct tcgacaatcg cctgcaccag ctccatgttg
     1561 ttcacattaa aggcccccag cgcgtacttt cccttcaggg ccttcgcgaa cagttctttg
     1621 gtgtgtgtta gtggcatagt agtaatgcga tgtctcgatt cggttatcga atcacgattc
     1681 gtgtcccaag cttacagacc gccggcggaa ttaaaaggcg aaaaagcgcg cctgggagca
     1741 cgcgatcacg cgcagcgcca attcatgaaa cggagcggcg acgcctgcct gccgcctggg
     1801 cgagggttat cggcgtgcag gcgtcgcaat cgagcgcccg accattcact cactcacgcc
     1861 cgaccattca ctcactcact ggcccttggg ggtgtcttgg tccgacccag ccaggcgaag
     1921 aacccgacca ggagaacgac gcagactagg atggtgagac ggaagaggag ggttgtggat
     1981 tgggcggggc gagcgttgat atcgagtgcg agatcggccc acggctggac gtggagcggt
     2041 cttgagaacg ttaataatcg gccctgctcc ggaattgtca ctcggatcgc tgccggactc
     2101 gccgccgctg cgtcctgctg ccgaatcaat cgctccgcca aacgcatctg aaccgcattg
     2161 tcctcggccg tgttgcgctc gatgagttgt ttggcctctt gctgcgtata gttggcggcc
     2221 tggctctcac gcagggcgcg cggtgtggcg gcgaggaccg acggttcacc cgccactttt
     2281 gcctggcgca cgttgaggcc gacgagggcc tgctgcatct tgaggttgtg aagttggacg
     2341 cgggcgtctt cgttgaaggc gttgtcgtgt tgggaaaggc cataggcggt ggtgaaggcc
     2401 cggcgggctt gttctggatc gccctcgacc aggagacgat tggcccagtt caggaactgt
     2461 tcggcttcct tggttttggc ttgttggaga gcggcctcgt tgcggatgta ggtttcgagg
     2521 tcgacggcga ctccgtcggt cgggccctgc tccggctgca actggagcga gcctctccaa
     2581 ctcgagaggc gccattgctc gccgaggaag acgcgccagg agatgttctc gaggggaaga
     2641 tcgaaccgcg gtcccagcaa ctcgagattc agcgccctcg aactgccggc tcccgcccga
     2701 gccgcgtaga agaactcgac cagcgtgggt tgtccggtct ttgcatgctg ttcgagcggg
     2761 atgagcacct ggttggtctc ccgccagggc cagacgctgc tttggttgac aaacgcgaac
     2821 cagaaacgcg ccttgtcggg caaggtcagg tggagcagcc gcttgtctcc cggcgtcagt
     2881 tgaatgcgcg cttgcgtcag cacgatcccg tcatcggaca cgacggacga gagcgtcagg
     2941 ctgtcgacgc gcgcggggag cagcttggcg gcggcgtgcc gctcgatttg caccggcagg
     3001 cgataggcgg gctcgaccag ccggaacgtg taattcgcgg cggtggcggc gacatcctgc
     3061 tgcagggcgc gcgggatcga ctgccactcg gcgggctgga gcgcctcggg cggcgaatcg
     3121 acgcgcacct gcagtcggcc gccggcctgc accgtgagga aaccgcgctg catgttcgca
     3181 tcgagggctt cgaccccgac gatgccggtc tcggccagcc ggtcgccgag gggcaggttg
     3241 tatgtcacct ggaggaggta cttgccgaag acccggcggt tgagcttgat ctcccaatcg
     3301 cgcgtgcccg cgccggcctg cccttcgagc ggacggaaat cggtcagttg ctcgccgcgg
     3361 aaccggacgc cctcggcgtc ggcgggcaag cggacgccca gggtcttgat gccggtgttc
     3421 tcgatttcgt actggagatt gactgcgacc ttgagctgcg cgtcgccgat gacgacatgc
     3481 tgaaggctgg cgacttgcgt ccaggcatcc atgcgctcga gatccagggt gaggctccaa
     3541 tcggactgca gcaatcgaaa cgccagaact cctttctgcc gaacgccggc cttgagcgga
     3601 tcgagctgcg tgacgccctc gcgcgaggcg atctggaggc gcaatccctg ctcaggcacc
     3661 agcagcaact gaccgcgttg tttgccggct tcgcgcacgc ccaggcgcgg caccgcccag
     3721 cccgttgcgg atcgcacgcc ggggccgctc aggctgactg cgaaggtctg tttgccctcc
     3781 gtcttgctct tcagatgcag ggtgatcgcc cgcccggcat ccgtcttcag ctcggtccag
     3841 tggctcaagg cggggccgct gatggactcg acatcgaggc cggtcggcag gaggaaactg
     3901 agcttgaaga tgccggcccg cgtcacctcg acctcgatat tggccgccag caccgtccga
     3961 tcctcgccca gcgacagagt ctgctccgcc tcgacccgga tgtctggctg gaccgctgac
     4021 gcctcgagcg tgaggttggc cgcggcatcc gagtagcgga acgcgcggcg cagggtcaga
     4081 ccggcaatgg cgggtcggag cggatcgagc atggccgcgg ggaaatcctc gaggttgatg
     4141 ggcgaaaacg actcggctcg gacgtcgtcc aattgcacct cgttgcccgt ggcgacgccc
     4201 agcagtccca gttgacccgc cgcgccccgc accccgagca agccggcggt ctgctcgaag
     4261 ggcagcgggc cggtggcgat ttgcgatttg acgagcaggt tgaacggttt ggactgcgcg
     4321 ggcgtgaggc gcacccgcaa ctgccggctc tgcggatcga atcgccacag ggaaagcccc
     4381 ggggcgacga catcggtgat ggtcgcgcca gcaggcacat cgaactcgag ttcgctcaac
     4441 tccccttggg cgggccgcaa ctggacctgg tgcaacgctt cgaccacgcc cgcgccgggc
     4501 acgaagagtt ggctcaactc cgcatagaac accgccttct cgagccgggt gtcgcgactg
     4561 cggggcttcc atgcaatcca cggcgcgcgc gcaggcgcca gcaccaggcg cgcgacggtg
     4621 ttcgtggacg cgccggaggc gacggattgg accgacaccg cctggggggc gttgatttcg
     4681 acgtcgagtc cgaccagggt gagcgtgagg cggttcacca ggccatgcgc cgtcggcaag
     4741 gcgaacgcgc gctcgccttc gcgagtgacg gtctggacct ggtaatcgag ccggatttcg
     4801 cacaggccgg tctgctcggc cagcagtgcc gtgtcgcgcc agtcggccgc ggcggtctgc
     4861 accaaccgcg cgctgttggt cgggtactcg atccgcgtga gaacacccgg cgcgtgcagc
     4921 agcgggagaa cgtccccctg gcgcgcctgc cagcggactc gggccgatcc gatggcatac
     4981 tggccctcga cgcggacctc ctggaccacc gattcggcca gaggcggcgt cttgttcggg
     5041 acgggaccgg tggcctggtc ggcctgggcc agggtggcgg cggattcgag caaccaggcc
     5101 ccgaacatga gcagggcggc cgccgcggca gcgcttccgg tgtcgggagc gggcttgcgc
     5161 ggcgcccgcc accagcgcca cagccagggc acaaacacgt gaagcacgac gaaggcaagg
     5221 aggacgccga agaacagcgg cgcgccattg ggccagcgca acgccgccca taacatcaag
     5281 acccaaccca gcacgcgcgc gaggactcgc aaccaggcct tcgagctggc caggccatag
     5341 agccagagca gcacgccggg cagggccggc gccagagccc acgcagacgc gaaccaggac
     5401 ggtcccaccg acacgttcgc gatgtcgagc gcgagggcgc tgtccgcctg ttgcaccggc
     5461 gcgacgaacc gcaggtcgcg cgggacctcg acccgcgccg atcgggccag gtcgaccaga
     5521 tccgccagcg cgatggcggc cagggcggcg gcggcgagcg cgagaatgcc tccgagcaaa
     5581 tggcggagcg tgagcttgtg gacgccctgg tcgcttgcca ggcgccagag ccaggcggcg
     5641 atgatggtca ggcccaaagc caggcccagg cccaaaagca gatcgtccct ggcttcgctc
     5701 gacagcaggc gctgcaggcc ggcaaagccg gtgacgcttt ctgtcgcgcc gaccggagtc
     5761 agcgggcccc ggcggcagac caggcgccgg ccggtgtcgg gaaccaggtg ccattccgcc
     5821 aacagcacgg gggcggatac gatcggggcg gcgacggcga tgcgtttgcg gtccttggcg
     5881 cgggcggcca gcttgatctg cagatcgttg acggcgtcgg cgtcggcgtg ttggggcagg
     5941 gggatcaggt gcgcgcggtc gctggtgacg gggacgacag gggcgttatt aacggtcacc
     6001 gaccagagct gggtgccttc gggaatgacc aggcggaggt ggggcgcgcc tttgttcttc
     6061 acgaaatagc gggcatcggt caccacttcg ccctcatcgg aaatgcgcgt gacgaacgag
     6121 gcgcggtcga cgacctggct gattgtctcg ctctgggcca gcggtttgag ttcgagctgc
     6181 aaattgaacg ggcgcgccgc gtagcggtag gcggcgagga ttggcgcgtc gaagaacagg
     6241 cggtattcgg cgggcacctc gcccgtttcg agcggcagaa gggcgttcga gaggttgacc
     6301 ggcttgacct ggaactggta tgcgctgacc acaatggtgt ggccctgctc cgattgggcg
     6361 tcgagggggc gggccccggt gaaggtgagg gtatcgcctt ggggcttgaa ggggcgttcg
     6421 taggttgcca gcagcgtgta ggctcccgcc acgggcgtgt gcaactggac ctggtaaccg
     6481 ccgtcggtct tctgccagct ccggacgtcc ttgccgctga actcgacgtt gaaatactcg
     6541 gccgacaact cgattttgaa cacggcgatc ggcgcacccg acacgacgta gttgatgatg
     6601 ctgctgccgt acgcgacccc ttctccgatc gagaataggt ggaacgtgtc ggcctggatc
     6661 gattgcggca ggcgctcgac ggccagcgtc gcttgccacg ccgcgtcgct catccggaaa
     6721 gccgcctgaa tgccggtcag cttcttcggg aagaacgcgc ccgcaatctc ggtcagcccc
     6781 tgcgttgcgc cgggcgtcaa ccgaaagccc gcgtccgccg tcacccccac atgcccgcgc
     6841 accgatttcg ccttgaggac gtcgatgcgc ggcaggcgcc aggccgattc ggcgaggggc
     6901 tggttgcgtt cgaggcgcaa ctgcacgacc tggcggccgg acaccggggc gccatagacg
     6961 agccggagtt gggcctcggg cagatcggcg ctctcggtcg ggaagtaatc gccgaggccg
     7021 ggcgcattca ggcgcgcgac ggcgtagccc ttgggtatgc gcagcagcat ctcgcgcagg
     7081 ggcgcctcgc gcacatccac ctcgaactcg gcatcgatcg ccagctcggt ctcccccagg
     7141 tgatactcga tcagagccga caccgccagt tcgggcagga tgttgtccgc ctgcatacgc
     7201 aactcgaacg cgccgcccga gaaacgatag gcaaacacct gcgtggtctg ctccgtcaaa
     7261 gccagcgtct gggtggcgtc ggtctgcggg aattgctcgg gcgaaatctg ggaaagcccg
     7321 ctcgcctgca acacctcgag ccgcacggcg ccttcattga cgatccggta gcagccgccg
     7381 aaccgcgtgg tgccttcggg caacacctgc atcgcctcga cagcctgcgg gaacgcaccc
     7441 agcggcgttt gcaactggac ctggaacgag aactggtcct tctgagcctc gttcaactgc
     7501 accgccagcc ggcggtcggc gctgttgggc agtggctcga ccacccagct caggaccgcc
     7561 ggcccctgca cgcgcgccac ctcgccggcg ccgcgcaggc gcaacgtgac acggttgagt
     7621 tcgccctgca tcactttgaa atcgagcaga gcggtctgcc gcatgagacc ggggctgatg
     7681 gcgatttgcg ccagcgcctc ggcggcgtaa aagagcgccc cctcggtctc gggccgggcc
     7741 tccttccacg cgagctgcac ctggccggtc ggcggaagaa agctcacaaa ctcctcgccc
     7801 gcgcgctcgg gccgggcgcc gtcggcgaac cggaactccg tgtcggcttt caatccctcg
     7861 atcgtcacgg gcgccagccc gccgggcgcg acgacgaaat cgagttcgtt ccagccgttg
     7921 gtctggcgaa cagccgcgtt gaacttgagt cggagcgcga actcgccgtc gcgatcgaac
     7981 accgcgacgt acttgttgcc ttcgagccgc caatgccagt ggggcgccgc attgacctcc
     8041 gtgagcgccg cccgccccgc cagcagggtc agcgagccgc ctttggggtt cttgacgcgc
     8101 gcgaggccgc tcagggtgaa ttcggcggag tcgccgctga gccggccttg cagacggaac
     8161 tggctgacga caacgcggga cgattcgggg tcggccgcct cgattgtcag gggcaagctg
     8221 tagggcgtgc cgtggaaacg gaacgcgatc gtttcaggag cgcccggcgt caccaccggc
     8281 cgcaagctct caggcaggaa cttggattcg atcggcgcca cgccggcggg attgcccact
     8341 tgaatcgcga gttcgggcgg tgtgtcgatg cggacgtagc cgtggctgag cgaggcgggg
     8401 tcgccgatca aggccagcgg ggtcacggtc tgcgggagat cgcccaagga ggtctcggcg
     8461 acggcctgca ccacgagcga cttgaccggc ttgtccgccc tcttcacacg cacgaccaga
     8521 ctgcgggcgc cgtcgggtcc gcgccgcacg ctccagtgct cgagggcgtc gctcgtcacc
     8581 cgcgtgatct cgccaacacc cgacagccag agctcgacct ccttcaactc gccttgaacg
     8641 gcgtcgatct ggatcgagat cgtgtgcgtg agcttggcgc gcgacacggc gatcgtctgc
     8701 tgaatagccg cgctcgggat gcacttctcg cgccccgagc tcagcccgcg caaatccgcc
     8761 tcgatgacca gacgcgcttt ctcggcaccg agttggccgt cgagagcgat tgcattgacc
     8821 tcgacagcgg tgggcgaagt cgcggcgggt tcggacgcgg gcgtcggggg cgggactatg
     8881 gccggggcag gatcgggatc gcccgcgaag gttgaagcgg ccagccacaa ggccagaaga
     8941 gccgacgcaa cgatgctctg cgctgcgatc ggatggacaa gggcgccccc gtgattgcga
     9001 tggcgatcga ggggcgggca gagcgcatcc cggcgttcct tcagaatatg ggagaacctt
     9061 gcagattcgg ttgtgtcttt gccccgccgc ggtccgagaa accggtgcat ccggatgttg
     9121 tcggtgcttg tgtcgcccgg tgagggcacc aggcctacac cggttgcaga ctcccgctgt
     9181 cggccgcgtg tccccacgcg gcgcaaactc gccccccaca gacaatctcc agatgcactg
     9241 cagaaagacc tctgttggcc caatgtcgtt cggatagtca tgttgttgcc ttccatgaac
     9301 cgttgttccg tagccgtccc gcgacgcccg atcggtccgc gccatctcgc tccgccaaag
     9361 gcgcattcac gttcactgaa cacactcttc attgtccgct gatcgctttg gcggcgcgga
     9421 gcatggtctc gagttgcctc ggccgggcgt ccggaccgaa ggcgtcggtc acccccgcca
     9481 tggcgccgag cagcttatcg ggcgtgactt cgccggcgaa cgggctcgac gcgagccatt
     9541 cggcgaacgc ggcggaggtg acggccagac gcatggccgg actggactcc tcgagcccgg
     9601 gcgccgggcc ggtgtaaggg acgcgccagg cgtgttcgcg gtagaggccg ctggccgcct
     9661 cgcggtagcg cacccgcacg gtgccgacgt cgccctgacc gcgcggattg agttcgagga
     9721 tgtaaagggc gttgcctgac tcggccgcgc ccaactcggc ggcgtcgacc gtgttgtcgc
     9781 ggaactgctc cttcgtgagc tggtgtttgg cgtagccaat ctgccgccag gccgtcgccc
     9841 gctgcgggtt gaactcgacc tggaccttga cgtcagccgc ggcgacttcg agcgcgccgg
     9901 ccagttgatc cgccagcccg gtggccgctt cctcggggct gttcacaaaa ccgtagcgcc
     9961 cgtcgccatg gctcgagagc acttcgagca aatcgtcgtt gtacccttcc cacccgatac
    10021 cgaagcaatc gagcgcaatc cccttcttgc gagccgactc gaccttctgc ttcaacgatt
    10081 cgggatcgac gtcgcccagg ttggccgcgc cgtcggtgag caacaccaca cgattcacgc
    10141 cgtgactgac gaaatggcgc gccgcagtct cgtaggcaac gttcagcgcg tcttcgagat
    10201 tcgtccctcc ttcaggcgtc agtctgctga tcccgtccag aacctccctg gcctggcttc
    10261 cagaaacgcc atcgacccaa agccgggccg tgcgggcaaa ggcaacgaca ctgatccgat
    10321 cctgggcctc gagtttctgg gccagcaccg aaagggcttc gcggacgatg gcgacgcgat
    10381 cggcccgttc catcgagccc gaactgtcga gcaacaacac gagattgagc ggacgcaggg
    10441 ccgaccggcc ccgcgcggcg gtctgcaccg agaagcggat cgcctcgcgg ttgtgggcaa
    10501 aggggtagcg cgctcgttcc caggcgaacg cgaccggtgc gcctcgcacc ggttccggat
    10561 cccgatagtt gaacgcgttc aggaactcct cgctccggat gcgcgaggcg accgggagcg
    10621 cgccctgttc gaggctcgcc gcggcgagct tgaacgacac gtcagtcaca ttgagcgaga
    10681 aggtcgagaa cggattgacc gcggttgcca cttcaggctg cggttcgggc ggcggcgcgg
    10741 acggacggct ggcggctctc ggcaccccct tgaccacgac atcgcctgcg actcgcccgt
    10801 ttgcttcgag tgcctcggcg gagaccgcca cgttggtcat caatcccagt gcgtccacgc
    10861 ccgagtcagg cgccgcaacc ggaggcgctg caaccacaga cgctgcaacg tcaagcgcct
    10921 gcggttggag gccttgcccc tctccgttga ctaccgcgac cccgagacgc agatcggcca
    10981 cttgcttctc cgactccgca agcctgtcaa ccgcctgctc cttgtcggcg agctgacgcg
    11041 tcggcgcgga actcgacttc gcaaaccgag cccgaccgcg ttgttcgcca ctgaccaagg
    11101 ccccgcggcg aacgctcgaa tcgaccgatt tgccggcgct tggcgtgagc aatccaacag
    11161 gctcaggctc ggttgcgggt gcattggcgg cggcggtgac acgctccaac ctctccggtt
    11221 cgacctcaag ggatctgaac gcggcgccca gcatcggagc atcgcccagc accggcgctt
    11281 tggccacctc gatggcttct tgggcaggaa gcccgtatgc gtagtagccg gccccgtacc
    11341 gctccgcgtt ggccttggcg tccgatgaag acgcaggccc cgcaacgccc ccggaaggcg
    11401 gaacctcggc caccaagctt ggcatggtcg gcggggcggg cgtcgcaaca ggggccgcag
    11461 cggaggccgc agcgggggcc gccgcacgcc gggtgggacg gccagcctgg gccagactgg
    11521 ccagactgtc ggccccagcc acaagaaagc cccgatcggg cgcaggtgtt gaccggcttg
    11581 tgatgcggac tccggcatgg tcggatttgc tcaggcgttg ggcaacctcg gttgtgccgg
    11641 ccgagtccga atccggttca cccgctttgg cccgaattgt cgagaagaga tgcgtggccg
    11701 ggtcttggcc gcgatcggtg gcgacctgcc ggcccaagta aaagtcggtc gaactccgat
    11761 ctccgtccgt ttgcactccg ccaccgaatc cacccgatac acccgctccg ccgacacctc
    11821 ccattccacc cgccatgccc atccccccca ttccgccacc gccgctcctg ccccattcgg
    11881 ccgtgtcgaa gccattcaca agcgtgcctt cggccgccgc gtcgaagtgc caattctgct
    11941 gaatgtcccc gtacttggct acagtctccc cccacgtcat cgctcccgcg gactcttcac
    12001 cgcccctgat ctcggctgcc ctgttcttgg cttcgccatc cgcaaacgct aagtcggcgg
    12061 tgggcgtgcc cacgaaagcc ggggccggca actcgagcgg caggggggcg agttcactcc
    12121 gggcaggtgc ggacggcacc ggcgtgctcg cctcgggttg tggttgcatg gcgacgcttt
    12181 cgccgaggct gattgcatct gtctttcgct tttcgacggc cttggccaga ccggatgccg
    12241 cagtcgactc accggcgacc tcggcgagtt tcatcccttt tggcagaagc ccatagcgca
    12301 ttaacatccg aatatctgcc atgggctggg ccgactcggc gccgcttgat tcggccttgg
    12361 ctgccacctc gacacccttc agacgtggcg ctcctccagg ctgatacgtc tgcaattggc
    12421 gctcgcgccc ttcggtagcg ccctccgatt cgccgcgcat tggcatcccg gtcaccggat
    12481 cgatctgtga ttgggcatcc aacaggacat cggccggctc gtcggccggc tcgtcggcgg
    12541 gctggctgcc aagacgggtg accaaggcga cttgaggcgc gtcgtgtcgg tacgcgatcg
    12601 atcgaccttt gctcttcgcc tttgcgaggg aggggaggag caggctggcc atgacgatca
    12661 aaacggccgc ggcgctcagg ggaacgagcc aggaatgccg gcgggatctg ggcagatcga
    12721 accctcgggg cacgatcgtc ttgaagtggc gcagcaacga atctcgtcgc tcggctgaca
    12781 agcgcggccc gttggcgcat gactcggcgg acgcgtccgg cccgcctacg gcttggcgca
    12841 cgaggggcag gatggttcga agccgatccc gcagggccct cagggttgtg tcggcggcga
    12901 tcgcctgttc gacggcgcgg gcctcgtcag gcgccagttc gcccagcaac aacgccgtga
    12961 tcttcgactc gaagtcgtcc ggcggcagtg aaggcttgat cgggttcatg ggacaaagcc
    13021 ggctctttcg agttcggcgg caagagattt caaggcgtgg tgaaggatat agcccacgtg
    13081 gccgacagtg aggccggtgc gggcgctgat ttccttgtag gacaggtcct cgtgaaactt
    13141 gagctcaacc acctggcgcg cccgcgcgtc caggctcttc aatccgctcc gcaccaaacc
    13201 gatcccttcg cgccgcgcag tctgttcgtc cggatgcggt tgtgggtcgg gcgggtcgtt
    13261 cgaagggtcg aaggaaccgc cggaccgatc ccgggcgacg tcgaggaggg tgattctggc
    13321 ggtcttacgc tggtggttca gggcgaggtt atgggtggtc tggtagagcc agccccgggg
    13381 ttcgcgcact tccgcaaaat gggtgtgcaa gcgcataaag gattcctgaa caacatcttc
    13441 cgccgtatcg gcatcaggca ccagccggag cgcgtacctg agaagcggcg attcgagcgc
    13501 ttcgaaaagg tcttcgacgg actcgggtct
//
LOCUS       DAOQRO010000041        13527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3616_length_13527_cov_4.564133, whole genome shotgun sequence.
ACCESSION   DAOQRO010000041 DAOQRO010000000
VERSION     DAOQRO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_13527_cov_4.564133"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKK20_02730"
     CDS             complement(<1..927)
                     /locus_tag="PKK20_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNU98834.1"
                     /translation="MEQAVKDVFNQGAARANAAYQMGRRRFTVEPITRLEGHGKIDIF
                     LDDKGDVERAYMQVPELRGFEVFSIGRPAEDMPQITSRICGVCPTAHHMASVKCLDDL
                     YKVEPTSPGRKIRELVYNGFLFEDHALHVFVLGGPDFIVGPEAPPAERNIVGVIGKVG
                     LEAGKRVITMRRRVRELTAYFGGKVVHPVLGLPGGVSKPLDPADLPKFKQLAKDAVEF
                     AEWTVDVLKKIVLGNPEYVKMITSDAYTHKTCYMGMVDEQNRLNFYDGKLRVVDCNGK
                     EICKFTPHEYRDYVAEHVEPWSYMKFTFLKPRG"
     gene            complement(939..1913)
                     /locus_tag="PKK20_02735"
     CDS             complement(939..1913)
                     /locus_tag="PKK20_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNU98835.1"
                     /translation="MSKPKVAFYWCASCGGCEETIVDLAEDILGIVEKVDIVLWPVAM
                     DFKYKDIEAMPDKSIAATLLNGAIRTTEQEEMAHMLRRKSQYLIAYGACAHLGGIPGL
                     SNQFSREHMLKFNYEDAPSMANPKKTRPQLKFADGGREPTLPELHHVVRTLDQVVEVD
                     YYVPGCPPTPKLTNVAVTALLENRLPPKGSVIAPDIALCDECPRKASKPVNTSFTEFK
                     RPHQKLMDPNLCLLAQGIVCMGPSTRSGCEAACIRGGMPCTGCFGPTSRVRDQGLKIL
                     SSLCSNVAPKENGEIDKVLEGIPDPVGTFYRYGLAKSLMRRKVNLPVK"
     gene            complement(1906..2457)
                     /locus_tag="PKK20_02740"
     CDS             complement(1906..2457)
                     /locus_tag="PKK20_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNU98836.1"
                     /translation="MTKEWRPRIVAFFCNWCTYTAADLAGVSRMKYASNVRVIRLMCS
                     GRVDPQFVLDALTRGADGVLIGGCHPGDCHYAEGNYKMLRRFQLLKRLLRDMGIEEER
                     VRLEWISAAEGDKVKAVINEMTDQLLKVGPLGLPQRFEEWDKEVTRLEEEIRGGDAAG
                     AGCACAAKLQEPAAASKSEVAHV"
     gene            complement(2450..4498)
                     /locus_tag="PKK20_02745"
     CDS             complement(2450..4498)
                     /locus_tag="PKK20_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNU98837.1"
                     /translation="MQSTKENIRIGFYVCHCGHNIAAMVDCADVAKYVKGLPNVVLSR
                     DYKYMCSDPGQELIQKDIREYKLNRVVVASCSPLLHEHTFRSATAAGGLNPYYFHMVN
                     IREHDSWVHTDRGEATVKAKALAHAAIDRVAFHKALETKTVKINPDALVVGGGIAGIH
                     AAMTLANAGKKVYLVEREPSIGGHMAKFDKTFPTLDCAACILTPKMSEVGAHPNITLW
                     SYSEVAQVDGYVGNYKVRVKRKPRYISEERCIGCMDCISACVFKEPKFEDEFNEGLGK
                     RKPVYIPFPQATPQIVVIDPNVCLNFKRTKGPDGTPIVSDRCKKTCVEACGDKNAIDF
                     TQKEEIKEIEVGTIIVATGFKTFDSKRVPAYGYGIYKNVYTALEIERLVNASGPTGGE
                     VILREGKNPDGSPKHPKSIGIIHCVGSRDKKTNVWCSRVCCMYSMKLAHLLKEHTGAE
                     VYNFYIDIRAPGKGYEEFYNKLLDEGVHFIRGRVGEVTDWAMTPEEEGKLVIRVEDTL
                     AGYVRRIPVDMVVLSSGLEPQADAQDVRRLFNMSCGTEGFFLERHPKLAPVNTFTDGI
                     FIAGACQGPRDIPDTVAQAGAAAAEALVLIDKGFVEQEPNTAFILDEECSGCKSCIPL
                     CPYSAILYDETRKKAHINEALCKGCGTCVAACPSGSIVQNLFEDEQIFSEIQGVLAYD
                     "
     gene            complement(4538..5437)
                     /locus_tag="PKK20_02750"
     CDS             complement(4538..5437)
                     /locus_tag="PKK20_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNU98838.1"
                     /translation="MKYSYYPGCSLKGQGRAYEESLLPVMRHLGIELEELPDWNCCGA
                     TAYMAVDEGKACVAASRNLAIAEKAGLQQVLAPCSACYLVLNKTQHLIHDYPQIKSAV
                     DRAMTSVNLKYSTDIPVRHPLDVLISDVGVDAIKQKVTHPLKGLKVAPYYGCQIVRPY
                     ATFDDQFNPTSMDRILEALGATMVQFPLKTKCCGGSLTGTLPEAGLRLSYIILKEAIK
                     RGADVIATVCPLCLFNLDSFHGKIAAKWESARIPTVYFTQLMGLAFGMTAKQMGLQRG
                     LVPMKPLASAPACCAARTAAAAT"
     gene            complement(5449..6018)
                     /locus_tag="PKK20_02755"
     CDS             complement(5449..6018)
                     /locus_tag="PKK20_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNU98839.1"
                     /translation="MTRTIKYEADRVKGFGEEVMSVPGCEKLKDCIQCGTCSGVCPLS
                     IYMDFTPRQVIEMTRFDFKNEVLGSKTIWLCASCYACSVECPQQIGITDIMYELKQRA
                     IKEKVYPRGLPTPVLAQEFTKMVHDYGRVNEILLATKMYMKTNPLAAMGMSTLGLGMI
                     KTGRFSMIQEKIQGRADLARMLETVDEGK"
     gene            complement(6432..8237)
                     /gene="dnaG"
                     /locus_tag="PKK20_02760"
     CDS             complement(6432..8237)
                     /gene="dnaG"
                     /locus_tag="PKK20_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNU98840.1"
                     /translation="MTGLISPQLIEQIRAASDIVDVVGGYLPLKRAGANFVALCPFHR
                     EKTPSFHVNPQRQIFHCFGCQKGGDVFTFVREYESLSFVEAVRRLAERARIPIEFEQE
                     PGERQSRFRKDTLLEMHERIAQRWQAVLNNDAAGQVARDYLIRRGVTDEAVRLFRLGY
                     APDLWDDTVNWAKSKGYDPALVEEAGLITRKQGEGGTFGRHYDRFRGRLMFPICDEQG
                     RVIAFSGRILSGDEKTAKYVNSPETPIFTKGRVLFGLDKSKRAILDAGRVIVCEGQLD
                     LMACYMAGVQNVVAPQGTALTGDHCHVLKRYVDEAVLCFDADVAGQNAALRALDDLLQ
                     SGLAIRVATIPAPHDPDSFIRDRGAEAFRNLIENAQGFFDYLLDRLVGTNDGATDRGR
                     MAIVNGMAEYLHKTGNAVLVDTYAQKTAQRLGVSIEAVRAEFRKGPRPSPARRETTDA
                     SPVSEPAARPAPQESWLLRLLLGNDALVPLAADRLDLNWLQHAAVRDLIRRRLEAEAG
                     GTWRGVAVFLGQFEDAQSRQLITEAVSETLGTADPERLLHDVLRRLRDTALDREIVQV
                     NRQIADNALSDGQRFELLRRKQGLVAAKQAPLRPA"
     gene            complement(8245..8736)
                     /locus_tag="PKK20_02765"
     CDS             complement(8245..8736)
                     /locus_tag="PKK20_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNU98841.1"
                     /translation="MAATGKSCAIGRFAAFLSQRGLRLTAQRRAILDAVFGTHQHFTA
                     DELLVWARALDRSVSRATVYRMLPLLTESGLVRALDLGKNSKVFDPNYADHPNHNHLI
                     CENCDRIIEFESEEIARLAEDIGRRLGFEVTGQKLHLTGACVEFGARGTCAHKGRRHG
                     RSG"
     gene            complement(9116..11773)
                     /gene="mutS"
                     /locus_tag="PKK20_02770"
     CDS             complement(9116..11773)
                     /gene="mutS"
                     /locus_tag="PKK20_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNU98842.1"
                     /translation="MGDPQLTPMMAQYRRIKGELPADALLLFRLGDFYEMFFEDAQTG
                     AQLLNLALTSRNAVPMCGLPYHAAAGYIGRILKAGRKVAICDQLEDARPGRLVKREVT
                     QILSPGAHFDERMLAAERPNYLATVCRIGKTFGLALVDLTTADFRTTEPDTETSLLAE
                     LERVRPSEVLLPSEAADLAALLSSRSRARPSATPGQPPAASGARTEFGWVLSRYDDWV
                     FAPETAQFTLRDHFKVASLDGFGLRGRAAAMGAAGAALHYLSQQLRRDLAHLTRLSFY
                     QTSDYLVLDAATIRNLEVLEPLHRDAPRDASLYGALNRTVTPMGARRLREWLSQPLAA
                     ARPIRRRQQAIGAWVDHPAQLESFRRALVEVRDLERTLSRLSVGTGNARDLVALRLGL
                     EQVPRVRETLMTALDEAPASAPVFKELAASDSAQAAPDDEEGSRLVELAGQLSDCPEI
                     VDLIGRAIVDEAPMALKEGGLIRDGFDAALDELRRAAREGKDWIAQLQRKEIERTGIG
                     SLKIRFNSVFGYYIEVTKANLAKVPGDYVRKQTIANGERFITSDLKEMESRILGAEER
                     GVKLEYELFLRVRERVLGRVAEIQQTATALGELDVLAGLAETARLHNYCCPEVGEAGR
                     LVIRDGRHPVLDQNLAGERFVPNDTELGVETQQIGLITGPNMAGKSTFIRQVALLTLL
                     AHAGSFVPAASARIDLVDRIFTRIGASDDLARGQSTFMVEMSETANILNNATVRSLVI
                     LDEIGRGTSTFDGLSLAWSIVEYLHNQVGAKTLFATHYHELTELAGRLKRLRNYNVAV
                     REWNDQVVFLRKIVPGGTDKSYGIQVARLAGVPRDVLDRAKAILRNLEESELTPEGNV
                     RRPARHRIERETLQALEPPPQLDLFESRA"
     gene            12580..>13527
                     /locus_tag="PKK20_02775"
     CDS             12580..>13527
                     /locus_tag="PKK20_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98843.1"
                     /translation="MPARRLSSFFRASVRLIAGLVFATLALSPSAASPATLSSESVLI
                     GTCVGEERERGPHHQVIDSLATWRTRDGALITTTNRVTVLATGLFYRDAERWLPSREE
                     IVFTPRAAVAQTGPHRIECPLYLGANRVHALETCDGQKFRWQVLGLQYWDPESGRRVW
                     IARTRSAHAELAAPNKLVYRDAFDGVRADLLFEYAKASFAQDVVLRTQLPNPAQWGLE
                     SDRLRLEIVTEFLTAPEPRIDRVQVWQESDPIRAARMREPVLTHDRLVFGAYGMAARQ
                     VFWVQGTNPESDLGLELPVLHRWAMMDGRAMLLESVELDA"
BASE COUNT         2412 a         4550 c         3972 g         2593 t
ORIGIN      
        1 gccgcgcggc ttgaggaagg tgaacttcat gtagctccac ggttcgacgt gctcggccac
       61 atagtcgcga tactcatggg gcgtgaactt gcagatttcc ttcccgttgc agtccacgac
      121 tcgcagcttg ccgtcgtaga aattcagccg gttctgctcg tcgaccatcc ccatgtagca
      181 ggtcttatgg gtgtaagcgt cggatgtgat catcttcacg tactccggat tcccgagtac
      241 gatcttcttg agcacgtcca ccgtccactc cgcgaactcg acggcgtcct tggccaactg
      301 cttgaacttg ggcaaatccg ccggatcgag cggcttgctg acgccgccgg gcagcccaag
      361 caccgggtgc accaccttcc cgccaaaata ggcggtcaac tcccgcaccc gacgccgcat
      421 ggtgataact ctcttgccgg cctcgaggcc gaccttgcca atcaccccga caatgttccg
      481 ctccgccggt ggcgcttccg ggccgacgat gaaatccgga ccgcccagga cgaagacgtg
      541 cagggcatgg tcttcgaaca ggaaaccgtt gtagaccagc tcgcgaatct tccgtccggg
      601 cgaggtgggc tcgactttgt acaaatcatc caggcacttg accgaggcca tgtggtgggc
      661 cgtgggacac actccgcaaa tgcggctggt gatttgcggc atgtcctccg ccggcctgcc
      721 gatgctgaac acctcgaagc cgcgcagctc ggggacctgc atgtaggcgc gctcgacgtc
      781 gcccttgtcg tcgaggaaga tgtcgatctt gccgtgtcct tcgaggcggg tgataggctc
      841 gacggtgaac cgccgccgcc ccatctgata ggccgcgttg gcgcgcgccg ccccctggtt
      901 gaatacgtct ttgacagctt gttccatagc gggtgacctt atttgaccgg caaattgact
      961 ttacggcgca tcaagctctt cgcaagtccg tagcggtaga acgtgcccac cggatcggga
     1021 atcccctcga gcaccttgtc gatctcgccg ttttccttcg gcgcaacgtt cgaacagagg
     1081 gacgacagaa tcttcagtcc ctggtcgcgc acgcgcgagg tcggaccaaa acacccggtg
     1141 cacggcattc ctccccgaat gcaggcggcc tcgcaaccgc tgcgcgtgct gggccccatg
     1201 cagacgatgc cctgagccag caagcagagg ttggggtcca tcagtttctg atggggccgt
     1261 ttgaactccg tgaagctcgt gttcaccggt ttcgaggcct tccgcgggca ctcgtcgcac
     1321 aacgcgatgt ccggcgcaat caccgagcct ttgggtggca gccggttctc gagcagggcc
     1381 gtcaccgcca cattcgtcag cttgggcgtg ggcggacatc ccggcacgta gtagtcaacc
     1441 tcgaccacct ggtccagtgt ccggaccacg tggtgaagtt ccggcagagt cggctcgcga
     1501 ccgccgtccg caaacttcaa ttgcgggcgc gtcttcttgg gattcgccat gctcggggca
     1561 tcctcgtaat tgaacttgag catgtgctcg cgcgagaact gattgctcag gccggggatg
     1621 ccccccaggt gggcgcaggc cccgtaggcg atcaagtact ggctcttccg gcgcagcatg
     1681 tgggccatct cttcctgctc ggtggtgcga atagccccgt tgagcagagt ggccgcaatg
     1741 gacttgtcgg gcattgcctc gatgtccttg tacttgaaat ccatcgccac aggccagagg
     1801 acgatgtcca ccttctcgac gatccccagt atatcctcgg ccaggtcgac gatggtctct
     1861 tcgcagcctc cgcacgaggc gcaccagtaa aacgcgactt tgggtttaga catgagccac
     1921 ctccgatttc gaggctgcag cgggctcctg cagcttcgca gcgcaggcgc atccagcccc
     1981 cgctgcgtcc ccgccgcgga tttcctcctc cagacgggtg acctccttgt cccattcttc
     2041 aaaccgttgc ggcaagccca gcgggccaac cttcagcagt tgatcggtca tttcgttgat
     2101 gaccgccttg accttgtccc cttcggcggc cgaaatccac tcgaggcgca cccgttcctc
     2161 ctcgatcccc atgtcccgga gcaggcgctt gagcagttgg aaccgacgca acatcttgta
     2221 gtttccctcg gcgtagtggc aatccccggg atgacacccg ccgatcagca cgccatccgc
     2281 cccgcgcgtc agcgcgtcca gaacaaactg cgggtcgacc cgcccggaac acatgagccg
     2341 gatcacgcgg acattcgaag cgtacttcat gcgcgacacc ccggccagat cggccgccgt
     2401 gtaggtgcac caattgcaaa aaaacgccac aatccgcggt cgccattcct tagtcataag
     2461 ccagtactcc ttgaatctcg ctgaaaatct gctcgtcctc gaacaggttc tgcacaatcg
     2521 atccggacgg acacgctgcg acacatgtgc cgcagccctt gcacagcgcc tcgttgatgt
     2581 gggccttctt tcgcgtttcg tcgtacagga tcgccgagta cggacagagc ggaatgcacg
     2641 atttgcagcc cgagcattcc tcgtcgagaa tgaacgccgt gttgggctct tgctcgacaa
     2701 agcccttgtc gatcaacaca agcgcctccg ccgccgccgc gcccgcctgc gctacggtgt
     2761 ccgggatgtc ccgcggcccc tgacacgccc cggcgatgaa gatgccgtcg gtgaacgtgt
     2821 tgaccggagc gagcttcggg tggcgttcga ggaagaagcc ctcggtgccg cagctcatgt
     2881 tgaacagacg cctcacgtcc tgggcgtcgg cctgcggttc gaggccgctg gacaacacca
     2941 ccatgtcgac cgggatgcgc cgcacgtagc cggccagcgt gtcctcgacg cggatcacaa
     3001 gcttaccctc ttcctcgggc gtcatagccc agtcggttac ctcgcccacg cgtccccgga
     3061 tgaagtgcac accctcgtcc agcagcttgt tgtagaactc ctcgtagcct ttgcccggcg
     3121 cccggatgtc gatgtaaaag ttgtagacct ccgcgcccgt gtgctccttg agcaaatgcg
     3181 ccagcttcat cgagtacatg cagcaaaccc gcgaacacca gacattggtt ttcttgtcgc
     3241 gcgagccgac acaatggatg atgccgatgc tcttcggatg cttgggcgag ccgtcggggt
     3301 tcttgccctc gcgcaggatc acctcgccgc cggtcggccc ggacgcgttg accagccgct
     3361 cgatctcgag cgccgtgtac acgttcttgt agatgccgta tccgtacgcc ggaacgcgct
     3421 tggaatcgaa cgtcttgaag cccgtggcca cgatgatcgt gccgacctcg atctctttga
     3481 tctcctcttt ctgcgtgaaa tcgatcgcgt tcttgtcgcc gcacgcctcg acgcacgtct
     3541 tcttgcacct gtccgagacg atcggcgtgc cgtccgggcc cttggtccgc ttgaagttca
     3601 ggcagacgtt gggatcgatc acgacgatct ggggcgtcgc ctgcgggaag ggaatgtaga
     3661 ccggcttgcg cttgcccagg ccctcgttga actcgtcctc gaacttcggc tccttgaaaa
     3721 cgcacgccga gatgcaatcc atgcacccga tgcaccgctc ctcgctgatg tagcggggct
     3781 tgcgcttcac ccgcaccttg tagttgccca cgtagccgtc cacctgcgcc acctccgagt
     3841 agctccacag ggtgatgttc gggtgcgccc cgacctcgga catcttcggc gtcagaatgc
     3901 acgcggcgca atcgagtgtc gggaacgtct tgtcgaactt ggccatgtgc ccgccgatcg
     3961 acggctcccg ttccaccagg tacaccttct tgcccgcatt ggccagcgtc atcgccgcat
     4021 ggatacccgc gatcccgccg ccgaccacca aggcatcggg gttgatcttg accgtcttgg
     4081 tctcgagcgc cttgtggaat gccacccgat cgatcgccgc atgggccagc gccttggcct
     4141 tcaccgtcgc ttccccacga tcggtgtgca cccaggaatc gtgttcccgg atgttcacca
     4201 tgtgaaagta gtacgggttc aggccgccgg ccgccgtcgc gctgcggaag gtgtgttcgt
     4261 gaagcagagg cgagcaggac gccaccacca cgcggttcag tttgtactct cggatgtcct
     4321 tctggatcag ttcctgaccg ggatccgagc acatgtactt gtagtcgcgc gaaagcacca
     4381 cgttgggaag ccccttgacg tacttggcga catcggcgca atccaccatc gcggcgatgt
     4441 tatgcccgca atggcaaacg tagaaaccga tccgaatgtt ttccttcgta ctttgcatgt
     4501 cggtcttctt tcgattcgag caatcgtctc gggccgctca ggtcgccgcc gccgcggtcc
     4561 gcgcggcaca acaggccggc gccgacgcca gcggcttcat gggcaccaaa ccgcgttgca
     4621 gccccatctg cttggccgtc atcccaaagg ccaaacccat gagctgggtg aagtacacgg
     4681 tgggaatccg ggcggattcc catttcgcgg cgatcttgcc gtggaacgag tccagattga
     4741 acaggcagag gggacacacc gtggcgatca catccgcgcc ccgcttgatg gcttccttca
     4801 aaatgatgta ggagagccgc agccccgcct cgggcagtgt gccggtcagg cttccgccgc
     4861 agcacttggt cttgagcggg aactggacca tcgtcgcacc cagcgcttcg aggatgcggt
     4921 ccatcgaggt cggattgaac tggtcgtcga acgtcgcata cggacgaacg atctggcatc
     4981 cgtagtaagg cgccactttc aaccccttga gcgggtgcgt caccttctgc ttgatcgcat
     5041 ccacccccac gtcgctgatc agcacgtcga gcggatgccg caccgggatg tcggtcgagt
     5101 acttgagatt cacggacgtc atcgcgcgat ccaccgcaga cttgatctgg gggtaatcgt
     5161 ggatgaggtg ctgcgtcttg ttgagcacca ggtaacacgc gctgcacggc gccaacacct
     5221 gctgcaaacc agccttctcc gcaatcgcca gattccggga agccgccacg cacgccttgc
     5281 cttcgtccac cgccatgtag gccgtcgcgc cgcagcagtt ccaatccggc aactcttcga
     5341 gttcgatgcc gaggtgccgc atcacgggca ggagcgactc ctcgtaggcc cgtccttgtc
     5401 ccttcagcga acagccagga taatacgagt acttcatgcg tcggcctttc actttccttc
     5461 gtccaccgtc tcgagcatcc gcgccaagtc agcccggccc tggattttct cctggatcat
     5521 cgagaagcgc cccgtcttga tcatgcccaa ccccagtgtg gacattccca tggcagccaa
     5581 cgggttggtc ttcatgtaca tcttggtggc cagcagaatc tcgttcaccc gcccgtagtc
     5641 gtgcaccatc ttcgtaaact cctgcgccaa caccggagtc ggcaggccgc gcggatagac
     5701 tttctccttg atggccctct gcttgagctc gtacatgatg tccgtgatcc caatctgctg
     5761 cggacactcg accgagcagg cgtaacacga ggcgcatagc cagatggtct tgctccccag
     5821 aacctcgttc ttaaagtcga accgcgtcat ctcgatcacc tgccgcggcg tgaaatccat
     5881 gtagatgctc agaggacata cccccgaaca ggtgccgcac tggatgcagt ccttcaactt
     5941 ctcgcaccca ggcacgctca tgacttcctc gccgaagcct ttgacccggt ccgcctcgta
     6001 cttgattgtg cgcgtcatct ccataaaaaa cccgccttcc catcagccaa tcaggcccgt
     6061 ctcccccaag ccgtcaggac gcacctgttc gccctcgacg cctgcatcgc cgccatcccg
     6121 ataagatgcg tacatttctc tagcctcccc acaggccggg tgctaaccga gatttgttga
     6181 aaaagcatct ttttttggtt tgcccggacg gcagccgccc gaaagtagcc cgctgcgcac
     6241 cccgcaaacc gtggaaaact cccagtgttt ttgaccgttt tgagttgacg ccccggggcg
     6301 ggctgcgcgc tcattgcagg ccagccagcg gggcagaaac tgactcgaac cgagctcctt
     6361 cgctctcgtc ctcctgggcg gcctgacact cttgttccgc cgccgcatgg cgcgctgagc
     6421 cgagaccagt atcatgcggg ccggagcggt gcctgtttcg cggccaccaa gccctgcttg
     6481 cggcgcaaca actcgaatcg ctggccatcg ctgagcgcgt tgtccgcgat ctgccggttc
     6541 acctgcacga tctcacgatc cagcgccgtg tcccgcaacc gcctcaacac gtcgtgtaaa
     6601 agcctctcag gatccgccgt tccaagggtt tcggacaccg cctcggtgat gagttgccga
     6661 ctctgcgcat cctcgaattg ccctaggaag acggcaactc cgcgccacgt cccacccgcc
     6721 tccgcctcga gacggcgcct gatcaagtcg cgcaccgcgg catgctgcag ccagttcaag
     6781 tccagacgat cggccgccag cgggaccaaa gcgtcgttgc ccaaaagcag tcggagtagc
     6841 caggattcct ggggagccgg acgagccgcc ggctcggaca ctggcgacgc gtcggttgtc
     6901 tcgcgtcgcg cgggcgaagg cctggggccc tttcggaatt cggcccgcac cgcctcgatg
     6961 ctgacgccca ggcgctgcgc ggtcttctga gcgtaggtgt cgacgagcac ggcgttgccg
     7021 gtcttgtgga ggtactcggc catgccgttc acgatcgcca tgcggccgcg gtccgtggcc
     7081 ccatcattgg ttcccaccag acggtcgagc aggtaatcga aaaaaccctg ggcgttctcg
     7141 atcaggttcc ggaacgcctc ggcccctcga tctctgatga agctgtccgg atcgtgaggg
     7201 gcggggatgg tggcgacccg gatggccagg cctgactgca gcaaatcgtc caacgcccgc
     7261 aacgccgcgt tctggcccgc aacgtccgca tcgaagcaca gcacggcttc gtcgacatac
     7321 cgcttgagaa cgtggcaatg gtcgccggtc aaggccgtgc cctgaggcgc cacgacgttt
     7381 tgaactccgg ccatgtagca ggccatcaaa tccaactgcc cctcgcacac gatgacccgc
     7441 ccggcgtcca gaatcgcccg cttcgatttg tcgagcccga agagcacccg tcccttggtg
     7501 aagatcggcg tctcgggaga gtttacgtac ttcgccgtct tctcatcccc gctcaagatg
     7561 cgtccgctga acgcaatcac ccgcccctgc tcgtcgcaaa tcgggaacat caaccgcccg
     7621 cggaaccggt cgtaatgccg tccgaacgtc cctccttcgc cctgtttgcg ggtgatcagg
     7681 cccgcctcct cgacgagcgc ggggtcgtaa cccttgctct tggcccagtt gacggtgtca
     7741 tcccagaggt ccggagcgta cccgaggcgg aagagccgga cggcctcgtc ggtcacaccg
     7801 cgccgaatca gataatcccg cgcgacctgg cctgcggcgt cgttgttcag aaccgcctgc
     7861 cagcgctggg cgatccgctc gtgcatctcg agcagggtgt ccttgcgaaa ccggctctgg
     7921 cgctcgcccg gctcctgctc gaactcgatc ggtatccgcg cccgctccgc caaccggcgc
     7981 acagcctcga caaagctcag actctcgtat tcgcgtacaa acgtgaatac gtccccccct
     8041 ttctggcagc cgaaacaatg gaaaatctgc cgctgcgggt tgacgtgaaa actcggcgtc
     8101 ttctcacgat gaaacggaca caaagccacg aaattcgccc cggcccgctt caacggcaga
     8161 tacccgccaa ccacatcgac gatgtcgctg gcggctcgaa tctgctcgat caactgcggc
     8221 gagatcagac cagtcatgga cctctcaacc cgagcggcca tgccgccgcc ctttgtgcgc
     8281 gcatgtgccc cgggccccga attcgacaca cgccccggtc agatgaagct tttgacccgt
     8341 cacctcaaac cccagcctcc ggccaatgtc ctcggccagc cgggcgattt cctcgctctc
     8401 gaactcgatg atccgatcgc agttctcgca gatgagatgg ttgtggttcg gatgatccgc
     8461 gtagttggga tcgaacacct tgctgttttt ccccagatcc agcgcccgca ccaggccgct
     8521 ctcggtcaac aagggcagca tccgatacac cgtggcccgg gacaccgacc gatccaacgc
     8581 ccgcgcccaa accagcaact catcggctgt aaagtgctgg tgcgtcccga ataccgcatc
     8641 gagaatcgcg cggcgctggg cggtcagtcg aagccctctt tggctcagga acgccgcaaa
     8701 acgcccgatc gcacatgatt tcccggtggc ggccatgtcc gcactataag gagaggctgt
     8761 cccaagtcaa cgcgccgcct caatcggagg ggccaaaacg cggcctgcag cgggagtctc
     8821 catccgttgc aggcccggtg ccctcaccgg gcgagataaa accgacacct gtcagatgca
     8881 ccgacccgcc agcaaccttt agcaacatca caaggaaccc gcaacctggc gagttggcct
     8941 gcgagactgc tctctcttgt catatgagtg cgtgcgaatc gtgcagtata gccgaaagac
     9001 ctgtatttcg catccatgaa tcggaccagt tgagagccga gagtcgagag ttgtacgctt
     9061 ctggttcatg gttctcgcgc gcattcgacc gttgaatgtc aaagctcccc atgaattacg
     9121 ccctgctttc gaacaggtcc aactgcggcg gcggttcgag cgcctgcagg gtctcgcgct
     9181 cgatccggtg gcgcgcgggc cgacgcacgt tgccctcggg cgtcaactcc gattcctcga
     9241 ggtttctcag aatggccttg gcgcgatcca gcacgtccct gggcacaccc gccagacgcg
     9301 ccacctggat gccgtagctc ttgtccgtgc cccccggcac gatcttgcgc aggaagacga
     9361 cctgatcgtt ccattcgcgc acggcgacgt tgtagttccg caggcgcttg agtcggccgg
     9421 caagctcggt cagttcgtga taatgcgtgg cgaagagtgt cttggcgccg acctggttat
     9481 gtaggtattc gacgatgctc caagccaggc ttaggccatc gaaggtgctc gtcccccgcc
     9541 cgatctcatc caggatcacc aggctgcgca cggtggcgtt gttgaggatg ttcgccgtct
     9601 cgctcatctc gaccatgaac gtcgattggc cccgcgccag atcgtcgctc gcgccaatgc
     9661 gcgtgaagat gcggtcgacc agatcgatgc gtgccgaggc ggcggggacg aacgagccgg
     9721 catgggccaa cagggtgagc aacgccacct gccggatgaa ggtgctcttt ccggccatgt
     9781 tcggacccgt aatcagaccg atctgctggg tctcgacgcc caattccgta tcgttgggca
     9841 cgaaccgctc tcccgccagg ttctggtcca acaccgggtg ccgcccgtcg cgaatgacca
     9901 agcgccccgc ctcgcccact tcgggacagc agtaattgtg cagacgcgcg gtctcggcca
     9961 gcccggcgag cacgtccagt tcgcccagcg ccgtcgcggt ctgctggatt tccgcgacgc
    10021 gcccaagcac ccgttcccgc actcggagaa agagctcgta ttcgagcttc acaccccgct
    10081 cctcggcgcc gaggattctg ctttccatct ctttgaggtc cgaggtgatg aaccgctcac
    10141 cgttggcaat ggtctgcttg cggacatagt cgccggggac cttggcgagg ttggccttcg
    10201 tgacctcgat gtaatagccg aacaccgagt tgaatctgat cttgagcgag ccgatgccag
    10261 tccgctcgat ttcctttcgc tgaagctgcg cgatccaatc cttgccttca cgagcggcgc
    10321 ggcggagttc atcgagcgcc gcatcgaagc cgtcccgaat cagccctcct tccttcagcg
    10381 ccatcggcgc ttcgtccaca atcgcgcgtc cgatgaggtc gacaatctcc ggacaatccg
    10441 acagttgccc cgccaactcg acgaggcggg acccttcctc gtcgtccggg gcagcctggg
    10501 cggagtcgga cgcggccaat tccttgaaca cgggggccga cgccggcgcc tcgtcgagtg
    10561 ccgtcatcag ggtctcacgc acgcgcggca cctgctcgag gccaagacgc agcgccacaa
    10621 gatcgcgcgc gttgcctgtt cccacgctca gccggcttag cgtccgctcg agatcccgaa
    10681 cctcgaccag cgcgcgtcga aacgactcga gttgcgcggg gtgatccacc cacgccccaa
    10741 tcgcctgctg acgtcgccgg atcgggcgcg cggctgccag cggctgcgag agccattccc
    10801 gcagacggcg cgcccccatc ggagtcaccg tccggttgag ggcgccgtag agcgaggcgt
    10861 cgcgcggggc gtcgcgatgc agcggttcga gcacttcgag gttccgaatc gtcgccgcat
    10921 cgagcacgag gtagtcgctg gtctgataga aactcagccg cgtcaggtgg gccaagtcgc
    10981 gacggagctg ctggctcaga taatgcagcg ccgcgccggc cgcacccatc gccgcggcgc
    11041 gtccgcgcag gccgaagccg tccagcgaag ccactttgaa atgatcccgc agcgtgaact
    11101 gggcagtctc cggcgcgaac acccaatcgt cgtaacggct cagaacccag ccgaattccg
    11161 tcctcgcacc ggacgcggca ggcggctgtc ccggggtggc ggagggcctc gccctcgatc
    11221 ggctgctcag aagcgcagcc aggtcggcgg cttccgaagg gaggagaacc tcggagggcc
    11281 gcacgcgctc gagttccgcc agcaacgatg tctccgtgtc cggttcggtc gtccggaaat
    11341 cggcggtcgt cagatcgacg agcgctagac cgaaggtctt gccgatgcgg cagacggtcg
    11401 ccaggtaatt cggacgctcg gcggcgagca tgcgctcgtc aaaatgcgcg cccgggctta
    11461 gaatctgggt cacctcgcgc ttgacgagcc tgccgggcct cgcatcttcg agttggtcgc
    11521 aaatcgccac cttgcgcccg gccttcagga tgcgcccgat gtagcccgcg gcggcatgat
    11581 acggaagccc gcacatcggc accgcgtttc ggctcgtcag ggccaggttc aggagttgcg
    11641 cgccggtctg ggcgtcttcg aagaacatct cgtagaagtc ccccagccga aacaacaata
    11701 gcgcatctgc cggcagttcc cctttgatcc ggcggtactg cgccatcatg ggagtcagtt
    11761 gcgggtcacc catagcgttc cccgcggcag caagagcaat ccggtccctc cgacatgcca
    11821 gtcatgcccc caaagtcaat gtcccacccc gccattgtca actccaagaa cgccggagat
    11881 gaaggggcaa atccatgacg gcctcgcctc cgcgcagggc acctctcgga aaagtctggc
    11941 tgactccaac aggcgaaagc gaccagccca gcgcctgtgg ccagcccccc tgccgtgggt
    12001 tcgggagccg ccatccggtc ttgcgctttc gacgatcgtc ccggcatccc ggcgatgaag
    12061 gtctacgcgg atactggttt cctcgtgaaa ctggtgacgc acgaggccgg ttcggagcag
    12121 gcgatggccg agtttcgcag gcttgattcc cctcggttgg tcttccagcc gctgcacgcc
    12181 ctcgaagtct cacggtcacg gtcactccct cctgacacct tccgccgccg ccggcgcgtc
    12241 gaggcggttg ttgcggggtt cccgcgctgg acgtcacgac ccgggacgcg cggggtatcg
    12301 cttggccttg caatccgctc accgggcgag acaacaccga caacaaccgg acacaccgct
    12361 ctcgatggtc ggtcacacgc caggctttga ctcgagtggc ctctctggta ccgtttggct
    12421 gcgatgggcg aagcaggacc tgcaacaagg ggcaagacgc ccgcggcgaa gagcgtccaa
    12481 tgctcaggtt caaatcctgc tgtccgcggt tgcttcgctg gcccgcatat tgacacatcc
    12541 tcagcggacg tcgcggtcgg accccgcaag atgactgccg tgccggcgag gagactgtct
    12601 tccttttttc gcgcgtcggt ccgactgatc gcaggcctgg tgttcgccac gctggctctg
    12661 agtccgtcgg cggcgtcgcc cgccaccctg tcgagcgaat cagtgttgat cgggacatgc
    12721 gtgggcgagg agcgcgagcg cggcccgcat caccaggtga tcgacagcct tgccacctgg
    12781 cgcactcggg acggcgcact gatcacgacg accaaccgcg tgaccgtgct cgccaccggc
    12841 ctgttctatc gcgacgccga acggtggctc ccgtctagag aagagatcgt gttcaccccg
    12901 cgtgccgcgg tggcccagac cggtccgcac cgcatcgagt gcccgctgta tctcggggcg
    12961 aatcgggttc acgcgctcga aacatgcgac ggacaaaagt tccgctggca ggtgttggga
    13021 ctgcaatact gggacccgga aagcgggcgg agggtctgga tcgccaggac tcgctctgcc
    13081 catgccgagt tggccgcacc gaacaagctt gtgtatcggg acgctttcga cggggtgaga
    13141 gccgatttgc tcttcgagta cgcaaaggcc tcgtttgccc aagatgtggt gctgcggacc
    13201 cagttgccga atcccgcgca gtggggcctc gagtccgatc gcctgcgtct agagatagtc
    13261 accgagtttc tcacggcgcc cgagccgagg atcgatcggg tccaggtctg gcaagaatcg
    13321 gatccaatcc gggccgcccg catgcgcgag ccggttctta cgcacgatcg actcgttttt
    13381 ggcgcctacg ggatggcggc gagacaagtc ttctgggtgc aagggacgaa tcccgaaagc
    13441 gacctcgggc tcgagcttcc ggtgctgcat cgctgggcca tgatggacgg gcgcgccatg
    13501 cttctcgaga gcgtcgagtt ggatgcg
//
LOCUS       DAOQRO010000042        13478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3638_length_13478_cov_5.195187, whole genome shotgun sequence.
ACCESSION   DAOQRO010000042 DAOQRO010000000
VERSION     DAOQRO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3638_length_13478_cov_5.195187"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1368)
                     /locus_tag="PKK20_02780"
     CDS             complement(22..1368)
                     /locus_tag="PKK20_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98844.1"
                     /translation="MKTWLCAIALVLGGRIHAGSEVLRPSDVVFMYSGSAQTYREYGA
                     TVLAWGGKPTPKSLAEAAGVTWFGSVGMVTEFSAYHNRFPERFEEGLCRDLDGHPVKV
                     PWLTDHQSKGVPYWWCCTRQPLFRQFLRDRVVETIRAGAQGLHIDDHLGTAGGLWLGI
                     CFCDRCVDGFRAYLAGLSAADLDRLGIREPSSFNYRDEVRAWIAAGGGEAKRNATGHP
                     LWRHWSIYQGRGAAAFMLELRELAARTAGRPIPVGANAGLLWPGHLTDYQALDLFTAE
                     TDHRAETRKPTDLPLVAYRLAESMGRPYAATASGGDWAFIKEHNLPGLVRCWIAFSYA
                     TGQRLMAPHRQWCHTSEKGTHWYDGPSERFAPLYRFVRDHAGWFDGYATRADVGVVFP
                     YRSFAKNPHRWFELCNSLAAANVGYRLLVAGDETVDHPLKEAEIEAQGGSELGWGD"
     gene            1452..2342
                     /locus_tag="PKK20_02785"
     CDS             1452..2342
                     /locus_tag="PKK20_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNU98845.1"
                     /translation="MPGAYQRGMIHLGLHTDNWRPLSVGFEGACQKIAVTGLKHLEFA
                     AIHGQNFIAALGYDPGVSLQSNPRAIRRFLDGLGLKVSQIDGSYPMMGPSGSAFGVQY
                     VTQSIRFAAELGCPMVDTVDGAFETPGLNRKEVFRITCDNYRQCLSWAEDYKVVINVE
                     PHGPYTNDLDFMERLFKHFDSEWLRCNFDTGNTFIAGHDPLDYCKALRPYIVHCHIKD
                     VSAELAAAARGEETGIGSSIVPVGGGVNAENIKRCMDYLCETGWDGVVSIECHGSDEN
                     TAASVKFMREITRTRHARRK"
     gene            complement(2495..2812)
                     /locus_tag="PKK20_02790"
     CDS             complement(2495..2812)
                     /locus_tag="PKK20_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNU98846.1"
                     /translation="MNTKLFVGNLSFNTTENDLHDAFAAHGTVVEANLMVDRMSGRPR
                     GFGFVTMSTPEEAQKAITAMNGASLDGRALTVNIARPKEERPPGGGGRPRRDFGGGGR
                     GRY"
     gene            3131..4595
                     /locus_tag="PKK20_02795"
                     /pseudo
     CDS             3131..4595
                     /locus_tag="PKK20_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957555.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
     gene            4959..5300
                     /gene="hypA"
                     /locus_tag="PKK20_02800"
     CDS             4959..5300
                     /gene="hypA"
                     /locus_tag="PKK20_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNU98847.1"
                     /translation="MHEVSLMENAVRMAVETARASGGARVHRIRLRVGAMSGVVPDAL
                     RFASDMVCQGTPAEGAQLEIEEVPATCWCAHCAVEFATPDFLGECPHCHAVSGELRRG
                     TEMELASIEFS"
     gene            5302..6138
                     /gene="hypB"
                     /locus_tag="PKK20_02805"
     CDS             5302..6138
                     /gene="hypB"
                     /locus_tag="PKK20_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNU98848.1"
                     /translation="MCQECGCGIGGELSIGGRPASAAHSHSTHHGDGHSHQHEGGGGA
                     PHTHAHDDATAHQPGRQTVAVQRGLLEKNQRLAERNRGFFRAKKLLVLNVLSSPGSGK
                     TTLIQKTATLLASRLRVGVIVGDLATDNDAARLREAGIPVVQVTTGTVCHLEAEMIAK
                     AAERLDLDALDVLIIENVGNLVCPAAYDLGEDLRIVLLSTTEGEDKPLKYPPMFHSAD
                     VAVVTKSDLAAAAGFDRAQALAALHRIAHHARIFELSARTGDGMPAWIDYLEQQRASA
                     KC"
     gene            complement(6173..7627)
                     /locus_tag="PKK20_02810"
     CDS             complement(6173..7627)
                     /locus_tag="PKK20_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98849.1"
                     /translation="MAVGAWLTLAALARAAAGAPELEYLFPAGGQHGARFEVIGGGKL
                     DPWPVGVWTDDAGIQFAPGLTNGVFEVAIDPGVAPGGHLVRVYTSEGASKPALFMVGT
                     DPEVTRFPQSPANQAHAAAPPAPIPVTLNGQLRSADARLLFPIEAGADRVVRARFMAS
                     PLDSPAQASLALLKDGTPLVATLDPAGRDFELRCPIVAGGPLSLEVTALAGSYLGDAA
                     VFRVEVTDDPLVPIPRSHATSAISEGVTAFRPMTSARTLAIAGQTRGVISPEGRVIHY
                     SFMAHQNEQFRFVAKAGSIGSPLAPVVRVLAPDRTVLAESLPGADAELVWIAPSDGEH
                     LLAVTDAEGRGGTTYAFQLEVEEPRAHFSAVLEDHAFQLRPGGSCVCDVRVLRPVSSD
                     IVVSVSAIDLADGVTAGSVHAAAGLDRVRLVLSAAAAAKPSNSPFRIMVMTTGETPPR
                     IEFARFSLEPRHTDGSALAVRDCNQPWLTVRTAE"
     gene            7898..9355
                     /locus_tag="PKK20_02815"
     CDS             7898..9355
                     /locus_tag="PKK20_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNU98850.1"
                     /translation="MPNNQCSFWRRAVGLAIVSTLAVCAIEAATPPSTPNIVVIFCDD
                     MGYGDVGVFGARGYATPHIDRLASEGIRFSRFYVAQAVCSASRAGLLTGCYPNRIGIA
                     GALGPGSPIGIHSREVTLGELVKTRGYATAIFGKWHLGDQRQFLPIHHGFDEYMGLPY
                     SNDMWPNHPEAKSGTYPPLPLIEGDQIKEIMPDQTQLTTRYTERAVRFIERSKDRPFL
                     LYLAHSMPHVPLFVSGKREGKTTAGLYGDVIAEIDWSVGRILGALKRHGLDQRTLVIF
                     TSDNGPWLSYGNHAGSAGPLREGKGTCWEGGVRVPFIARWPGRIPAGALCSVPAMTID
                     LFPTIARLTGAHLPAHRIDGLDIWPLLSGQPNAQSPHEAFFFYLAGGQLQAVSSGPWK
                     LLLPHTYRTLGGQPPGRDGQPAKYVQRKVERPELYDLDTDIGETRDVASEHPEVVRRI
                     DALAERARADLGDALVQRVGAGVREPGRGGPASGR"
     gene            9587..10111
                     /locus_tag="PKK20_02820"
     CDS             9587..10111
                     /locus_tag="PKK20_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU98851.1"
                     /translation="MATVAEQLRTAREARRLTVQQIADITKMRADHVEALEEGNYNVF
                     TAPVYIRGFVRTCARLLKIDEASILETLEVELTQTEKFREHLSLIGQQKGPLDALMLI
                     LSRIRWRIVLPALGVLVILGIVLTIVRSIEEQQARDPLAGIQPGQYQPARSNLAETLA
                     LPNPGAARAPSGAR"
     gene            10108..11655
                     /gene="rimO"
                     /locus_tag="PKK20_02825"
     CDS             10108..11655
                     /gene="rimO"
                     /locus_tag="PKK20_02825"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNU98852.1"
                     /translation="MSGHENQARPLRVGLISLGCAKNLVDAEIMLGTLLRDGYEITND
                     LAASDAVIVNTCSFIDDAQEESVDAILESAHIRDALRPNQALIVSGCLPQRFRDDLPK
                     ALPEVDAFIGIDQVTAVARIVRQAVARRTAWLQKAEDARAPVLTVSRRPVFIPDCGTP
                     RFRLTPRHLAYVKIAEGCNHACSFCIIPRMRGSHRSRPQHDIVEEARRLLAEGAKELN
                     LISQDSTYYGLDLQPKADVRTMTDPKRFGDAIRALPERAATICSLLRALNDLPGDFWI
                     RLLYTHPAHWTDELIETIARCRKVARYVDIPLQHIHPVILEQMRRRTPPEHITGLLRR
                     IRADIPGIVLRTTFIVGFPGETDAHFNTLLEFIRTRRFERMGVFAYSQEKGTVAGRMP
                     KQVPESIRQRRRDQAMAVQKQIAREHASSQLGRRVRVLIEGTLDEARLDAVREMPWNN
                     SLARVGGRAKASWMRGVCAVGRTEADAPEIDGCVYVPGQLPPGAFVSVRILDHADYDL
                     LGEPVYE"
     gene            11648..12832
                     /gene="tyrA"
                     /locus_tag="PKK20_02830"
     CDS             11648..12832
                     /gene="tyrA"
                     /locus_tag="PKK20_02830"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HNU98853.1"
                     /translation="MSEMRTQSQAGGTPASAESAGGASMLADLRREIDAIDERIVDLL
                     GRRQSLTQSAVAIKRAAGLPVYHPAREENLISERRDQAKQAGLDPNHVEELYRCILRQ
                     SRMRQTVRAAATGVRPGARVLLVGGRGQMGRYFARWFGEAGYETRLLDVEDWPRARDL
                     CAEIDLALVGVPISLTTDVIKRLGPHLPPRCILADITSIKRAPVEAMITAHPGPVIGL
                     HPLFGPSTSSMDQQIVVATPGRDADGCQWLLDQLAAWGNIIMTIAAEEHDAVMDLVQG
                     LRHFATFAFGRFLAARQVNLARTLEFSSPIYRLEMGMVGRLFAQDPGLYADIILASPQ
                     RRALLKEFIESVGAARDLIDNADADRFCREFRQISEWFGPFCGQALRESTFLIDKLVE
                     RF"
     gene            12857..13201
                     /locus_tag="PKK20_02835"
     CDS             12857..13201
                     /locus_tag="PKK20_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HNU98854.1"
                     /translation="MGDPFTAFRAWGEPAASPPKPGGAIGGSSGDPVFIGAEPRHHDR
                     PFVGQGGERPRAGRYFEYRDGTPFLWMGDTWWNWTKPGIQFSSFQIYLRYGGRLKVGL
                     QPSAASSLFDYI"
     gene            13241..>13478
                     /locus_tag="PKK20_02840"
     CDS             13241..>13478
                     /locus_tag="PKK20_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98855.1"
                     /translation="MNGDSAREFHAPEDYPGNLQVKDWLPHIRRTDPNLVKRLPVLCP
                     FKAWSRPNITLERRARGPSSHSTAILHLRSIHRDM"
BASE COUNT         2460 a         4526 c         4189 g         2303 t
ORIGIN      
        1 aattcgttaa gtccctcttc ctcaatcacc ccatcccagc tcagaaccgc cctgggcctc
       61 gatttcagcc tctttcagcg gatgatccac ggtctcgtcg ccggcgacaa gcaggcgata
      121 accgacgttg gccgctgcca gggaattgca gagctcgaac caccgatgcg ggttcttggc
      181 aaaacttcgg tagggaaaca cgacaccgac atccgcgcgc gtcgcatagc cgtcgaacca
      241 gcccgcgtgg tcccgaacaa aacggtaaag cggtgcgaac ctctcggatg gaccatcgta
      301 ccagtgggtc cctttctcgg atgtgtggca ccattgacgg tgcggcgcca tcagccgctg
      361 gccggtcgca taggagaacg cgatccagca tcgcacaagt ccgggcaggt tgtgctcctt
      421 gatgaatgcc cagtcgccgc cgctcgcggt cgcggcgtag ggccgaccca tcgactccgc
      481 caagcgatac gccaccagcg gcaggtcggt tggctttcga gtctcggcgc gatgatcggt
      541 ctcggcagtg aacagatcga gcgcctgata gtcggtcaga tggcccggcc agagcagacc
      601 cgcattcgcc ccaaccggaa tcgggcggcc cgccgtccga gcggcaagct cccgcagttc
      661 gagcatgaag gccgcggcgc cgcgtccctg gtagatggac caatgacgcc agagcggatg
      721 cccggtcgcg ttacgcttcg cttcgccgcc cccggccgcg atccacgccc ggacctcatc
      781 gcgatagttg aaggaagaag gttctcgaat gcccagccga tccaggtctg ccgccgataa
      841 tcccgcgaga taggcgcgga aaccgtcaac gcaccggtcg cagaagcaaa tcccgagcca
      901 tagccccccc gccgtgccga ggtgatcgtc gatgtgcagt ccctgggcgc ccgcccgaat
      961 ggtctcgacc acgcggtcgc gaaggaactg gcggaacagc ggctgccgtg tgcaacacca
     1021 ccagtaagga acccccttcg attggtgatc ggtgagccac ggaaccttga ccgggtgtcc
     1081 atccaaatcg cggcacaacc cctcttcgaa gcgttcggga aaacggttat gatacgcgct
     1141 gaactcggtc accatgccca cgctgccaaa ccacgtcaca cccgccgcct cagccagcga
     1201 ctttggagtc ggcttgccac cccacgccag gaccgtggcg ccgtactccc ggtatgtctg
     1261 agctgagccg ctgtacatga acacgacgtc cgaggggcgg agcacctcgc ttccggcgtg
     1321 gatcctcccc ccgagaacca atgcaattgc acaaagccac gttttcataa tctgagccgg
     1381 catataccgc agcgccgtcg ggccgcaacc aaaaaggcca cggccagcga tgaatcgagt
     1441 tgacgctgga gatgccgggg gcgtaccaga gaggcatgat tcacctgggt ttgcataccg
     1501 acaattggag gcccctgagc gtcgggttcg agggcgcctg tcagaagatc gccgtcaccg
     1561 gtctcaagca cctcgagttc gcggccattc acggccagaa cttcatcgcc gccctcgggt
     1621 acgaccccgg cgtctcgctt caatccaatc cgcgcgccat ccgccgcttt ctcgacggcc
     1681 tgggcctgaa ggtctcgcag atcgacggct cctacccgat gatggggccg agtggatcgg
     1741 cgtttggggt tcaatacgtc acccagagca tccggttcgc cgccgagttg gggtgcccga
     1801 tggttgacac cgtcgacggc gcgttcgaga cgcccggcct gaaccgcaag gaggtcttcc
     1861 gaatcacatg cgacaactat cgccagtgct tgtcctgggc cgaggactac aaggtcgtca
     1921 tcaacgtcga accgcatggg ccgtacacaa acgacctcga cttcatggag cgcctgttca
     1981 aacacttcga ctccgagtgg ctccgctgca acttcgacac cggcaatacg ttcatcgccg
     2041 gccacgatcc gctcgattac tgcaaagccc tccgcccgta tatcgtccat tgccatatca
     2101 aggacgtcag cgccgagttg gctgcggcgg cgcgcggcga ggaaaccggc atcggcagct
     2161 ccatcgtgcc ggtgggcggc ggcgtcaacg cggagaacat taaacgctgc atggattacc
     2221 tttgcgaaac aggctgggat ggggtcgtct cgatcgaatg ccacggatcg gacgagaaca
     2281 ccgccgccag tgtcaagttc atgcgcgaga tcacccggac ccggcacgcc cgacggaagt
     2341 gaaaaaaaag agaaagcccc tcgcgaggct ctcgctcggg agagcctccg aggggcttgc
     2401 tgcaaagggt tgaggcgcta ccgggagatc gagtccgcct gtgtcctccg acccaacggt
     2461 tcccggagga acctatggcg tcggaaactt ggtcttagta ccgaccccga ccgccaccgc
     2521 cgaaatcacg gcgcgggcgt ccgccgccgc cgggagggcg ctcttccttg gggcgggcga
     2581 tgttgacggt caaggcgcga ccatccaagg aggcgccatt catggcggtg atggccttct
     2641 gtgcctcttc gggggtgctc atggtgacaa atccaaatcc gcgcgggcgc ccgctcatgc
     2701 gatcaaccat caaattcgct tcgaccacgg tgccgtgcgc ggcgaaggcg tcgtgcaggt
     2761 cgttttcggt cgtgttgaag gaaaggttcc caacaaacaa cttggtgttc atgtgatgtt
     2821 ccgctattcc tgacttgtct gtgctctctc cagactgttc ccgattatcg gggttcaaat
     2881 catcactgaa ccgagttcac ctgactgatt ttccatgccg tggaggctgc tcatttgaca
     2941 gaccgcggga aaatgccgat tcccgagcaa atagcaagct ttaatttttg gttttggcgc
     3001 gctcgcgcgg ttcctacccg gggaaaagga tgccttcttt gacgatgcgc accggtcgca
     3061 atgcccgaag tgcgcatgcc cgtgccgcca cgttgacacc ctcaggaccg accgctagtt
     3121 tgaaccggtc gtgagagtgt tgcaggagtc cctcatcgag ctgcttcgcc gcacctcggc
     3181 cgaaatgccc gaggacgttc aacaggccat cgtccgggcc ctccaaaccg agcgccaggg
     3241 caccatcgcg gagtcgactc tcaagatcat cgaacggaac atcgaactgg cccgccgcaa
     3301 gtcgcagccg atctgccagg atacaggcag cattctcttt tacgtcgacg tcccggtgag
     3361 tttcgacgag atcgccttcg ccgccacggc ttgcgacgcg gtgcggcagg ccacagccaa
     3421 cggctggctg cgtcagaact cggtcgacac gctcaccggc aggaatgacg gcaccaatgt
     3481 cgggcccgga tcgcccacgt tccatttcca ccagcgcgcc tcgcccggga tcgatgttcg
     3541 actcatcctc aagggcggcg gatgcgagaa cgtgggctcg cagtactccc tcccctgcga
     3601 gcggctcgaa gcggaccgcg acctcgatgg gtgtcgcaag gtcatcctcg atgccgtggt
     3661 ccaggcccag gggcgagggt gcggaccggg ggttctgggg gtttgcctgg gcggggaccg
     3721 agccacgagc tacgcgttct cgaagacaca gttcctgcgt cttctggatg accgcaatcc
     3781 ggtccctgaa ttggatcggc tggaacagga catcctgcgc acggcgaacc tgctggggat
     3841 cgggccgatg ggattcggcg gccggaccac cctgttgggc gtcaagatcg gcgccatcaa
     3901 tcgcctgccg gcctcgttct tcgtcagcgt cagctacatg tgttgggcct accgccgaca
     3961 gggcgtgacg ctgagtccgg acggtggaat tgaacgatgg ctctattgac ccgcccatga
     4021 tccccctctc ctttcccctg accgaggagc gcatccgcgc gctccgcgtg ggcgacgaag
     4081 ttgaatgcac cggcctcctc ttcaccggac gcgatgccgt gcacaagtac ctggcccgcg
     4141 gcggcgcctt gccgcccggc gtcgatctgc gcggcggcgt cctctaccat tgcggcccag
     4201 tcgtgttgaa ggaccccgac ggtgcctggc gagtcgttgc ggccggcccg accacatcgg
     4261 cccgagagga accgtaccag gccgatctta tcgcgaagtt cggcctgcgc ggggtcattg
     4321 ggaaaggtgg gatgggagaa cgcacgctgg cagcctgcgc ggcccacggg tgcgtctacc
     4381 tccatgccgt tggcggcgcc gcccaggtgc tggccgagtg cgtcaaacgc gtccgcgcag
     4441 tccacttcct cgaacaattc ggcgcgcccg aagccgtctg ggagttcgag gtcgaccggt
     4501 ttcccgcggt cgtcaccatg gacgcccacg ggcattcgct gcatcgcgac atcgaggccg
     4561 ccagccgcgc caagttgatc aaataccgag tctgacatcc gggctctggc aaaaccgcgc
     4621 ccggaaaggg aaccgggagc attctgtcgg tcctgagaat ggtctctcag gcgaacagtg
     4681 cgatgcagtt ctcgatctct cgaacctggc gcggttttgc ttctctagcc cgcatagcag
     4741 ttctcttgcg aagtctgagt gtcctgtgca ccatcggggt gtgaaaaata tgcgggctcg
     4801 tgtgacttcc ttctcgaaat ctgtcttgaa tgagtcaagt ttttgcgccg cgcagcggcg
     4861 ccccttacgg gccgtcgctt gccagtgact aacgcgaacg cccagtccaa gcgatctggc
     4921 catgtgcctc gccggtctga gacggagcct cgctaaccat gcacgaagtc agcctcatgg
     4981 aaaacgccgt ccgcatggcg gtcgaaaccg cccgtgcctc gggcggcgcg cgcgtgcatc
     5041 gcatccgttt gcgggttggc gccatgagcg gcgttgttcc cgatgcgctg cggttcgcca
     5101 gcgacatggt gtgccagggg actcccgccg agggggccca gctcgagatc gaggaggtgc
     5161 cggccacctg ctggtgcgcg cattgcgcgg ttgagtttgc cacacccgac tttttgggcg
     5221 aatgtcctca ctgccacgcc gtcagcggag agctgcgccg cggcacggag atggaattgg
     5281 catcgatcga gttttcctaa catgtgtcaa gaatgcggat gcggaatcgg gggtgagctg
     5341 agcatcggag gccgtccggc ctccgcggcg cacagccatt cgactcacca cggggacggg
     5401 cacagccacc agcacgaagg cggcggcgga gcgccacaca cccatgcgca tgacgatgcg
     5461 accgcacacc agcccgggcg ccagacagtc gccgttcagc gcggcctgct cgagaagaac
     5521 cagcgcctgg ccgaacgcaa tcgcggcttc tttcgcgcca aaaaactgct ggtcctcaac
     5581 gtcctctctt cacccggctc gggcaagacc acgctgatcc aaaagaccgc aaccctcctg
     5641 gcatcgcgcc tgcgcgtggg cgtcatcgtc ggcgatctcg cgacggacaa tgacgccgcg
     5701 cgcctgcgcg aggccgggat tcccgtggtg caggtgacca ccggcacggt gtgtcacctc
     5761 gaggcggaga tgatcgccaa ggccgccgag cgactcgatc tcgacgcgct cgacgtcctc
     5821 atcatcgaaa acgtgggtaa cctcgtctgc cccgccgcct acgacctcgg cgaagatctg
     5881 cgcattgtcc tcctctccac aaccgagggg gaagacaagc ccctcaagta cccgcccatg
     5941 ttccactccg cggatgtcgc ggtcgtcacc aagtctgatc tagccgcggc cgccggattc
     6001 gaccgcgccc aggcgctcgc ggccctccat cgaatcgccc accatgcccg aatcttcgag
     6061 ctgtccgcgc gcaccggcga tggcatgccc gcctggatcg attacctcga acagcagcgc
     6121 gcatccgcga aatgttgact tccccgccag ctcctgaatc gcggtcgcgt tctcactccg
     6181 cggtccgaac cgtcagccaa ggctgattgc agtcccgcac cgcaagcgcg cttccatccg
     6241 tgtgccgcgg ctcgagggag aaccgggcga actcgatccg aggcggcgtc tcgccagtcg
     6301 tcatcaccat gatccggaac gggctgttgg acggcttggc cgcggcggcg gcggacagga
     6361 ccagacgaac ccgatcgagc cccgcggcgg cgtgcacgga gcccgccgta acgccgtccg
     6421 ccagatcgat cgcggaaacg ctcacgacaa tgtctgaaga cacggggcgc agaaccctca
     6481 cgtcgcaaac gcatgacccg cccggcctga gctggaaggc gtgatcctcg agaaccgcgc
     6541 tgaagtgggc tcgaggctcc tcgacttcga gctggaaggc gtaagtcgtc ccgccgcgtc
     6601 cctccgcgtc cgtcacggcc aggagatgtt caccgtcgct gggcgcaatc cacaccagct
     6661 ccgcatcggc tccgggaagc gactcggcca gcacggtgcg atccggggcc agcacccgca
     6721 caacgggcgc cagcggggat ccgatgctgc cggctttcgc cacaaaacgg aattgttcgt
     6781 tttgatgggc catgaaactg tagtgaatca cccggccttc aggcgaaatc accccgcggg
     6841 tctgccccgc aatcgcaagc gtccgcgcac ttgtcatcgg acggaaagcc gtaacaccct
     6901 ccgagatcgc gctcgtcgca tgcgatcggg gaatcggcac cagcggatcg tccgtcacct
     6961 cgactcgaaa gaccgcggca tcgcccaggt aactcccggc cagtgccgtg acttcgagcg
     7021 aaagagggcc gccggccacg atcggacaac gaagctcgaa gtcgcgtccc gccggatcga
     7081 gcgtggccac aagcggcgtg ccgtccttca agagagcgag actggcctgc gccggcgaat
     7141 cgagcgggct ggccatgaat cgcgcccgca cgacccgatc cgccccggcc tcgatcggaa
     7201 agagcagccg cgcatccgcc gatctaagct ggccgttcag tgtgaccggg atcggcgccg
     7261 gcggcgccgc ggcatgcgcc tggttggcgg gcgattgcgg gaagcgggtg acctccggat
     7321 cggtgcccac cataaacagc gccggcttcg atgcaccttc ggacgtgtaa acgcgcacca
     7381 aatgccctcc cggagcgaca cccggatcga tcgcaacctc gaacacgccg ttggtcagac
     7441 cgggagcgaa ctggatgccc gcatcatcag tccacacccc aaccggccac ggatcgagtt
     7501 tgcccccgcc aatcacctcg aatcgcgccc catgctgtcc gccggcggga aacaaatact
     7561 cgagttcagg cgctcccgcg gcggcgcggg ccagcgccgc cagcgtgagc cacgcgccga
     7621 cagccactct cccgcgccgt ctgatcgagg gcgcagcggg gcagggcggc ttggaagaga
     7681 tcacgccacg actctagtcc tggcgtcgcc gcctgacaat ggggtcacat cttcattttt
     7741 gacatttgcg aatgcggctg atgcgaaccg gcggctgatc aggccggctg aggtccgccg
     7801 ccgacgcagt cagcacacaa atgtcaaaaa tgaagatgtg accccaaaga gggagtgaac
     7861 aaaccgccgg ctcgagccgt ctgtatatcg agtccttatg cccaacaacc aatgttcgtt
     7921 ttggcgtcga gccgtcgggc ttgcgatcgt ttcgaccctt gccgtctgcg ccatcgaggc
     7981 cgcaacgcct ccctcaaccc ccaacattgt cgtcatcttc tgcgacgaca tggggtacgg
     8041 cgacgtgggg gtcttcggcg cccggggtta tgccacgccg cacatcgacc gtctggcatc
     8101 cgagggcatt cggttctcgc gcttttatgt cgcgcaggcg gtctgctccg cttcgagggc
     8161 gggcttgttg accgggtgct atccgaatcg gatcggcatt gccggcgcgc tcgggcccgg
     8221 ttcgccgatc ggcattcatt cgcgggaagt cacgctgggc gagctggtca agacgcgcgg
     8281 gtacgcgacc gccatctttg gcaagtggca cctgggtgat cagcgccagt tcctcccgat
     8341 ccaccacggc ttcgatgagt acatgggcct gccctactcg aacgacatgt ggcccaacca
     8401 cccggaggcg aagtctggca catacccgcc gctgccgctg atcgagggcg atcagatcaa
     8461 ggaaatcatg cccgaccaga cgcaactcac cacacggtac acggaacgcg ccgtgcggtt
     8521 catcgaacgg agcaaggacc ggccctttct gctctacctg gcgcacagca tgccgcatgt
     8581 cccgttgttc gtcagcggga agcgcgaagg caagacgacc gcgggcctct acggagacgt
     8641 gattgccgag atcgactggt ctgtggggcg gattctcggc gctttgaaac gtcacgggct
     8701 ggaccagcgc acgctcgtca tattcacgtc cgacaacggg ccctggctct cgtacggcaa
     8761 ccacgccgga tccgccgggc cgttgcgcga aggcaagggg acctgctggg aaggcggagt
     8821 ccgtgtgccc ttcatcgccc gctggcccgg ccgcatcccg gcaggcgcac tctgcagcgt
     8881 accggccatg acgatcgatc tgttccccac gattgcgcgg ttgaccggcg cccatttgcc
     8941 cgcgcaccgg attgacggcc tggacatttg gccgttgttg tccggccaac ccaacgcgca
     9001 gagcccgcac gaggccttct tcttctacct cgcgggcggc cagcttcaag cggtctcgag
     9061 cggtccctgg aaactgctct tgccgcacac gtatcgcacc ctcggcggac agccgcccgg
     9121 acgcgacggc cagcccgcaa aatacgtgca gcgcaaggtc gagcgccccg aactctacga
     9181 cctcgacaca gacatcggcg agacgcgcga cgtcgcttcg gaacatccgg aggtggtccg
     9241 gcgcatcgat gcgctggctg aaagggcgcg cgcggatttg ggcgacgcgc tcgttcaacg
     9301 cgtcggagcc ggcgtgcgcg aacccgggcg cggaggcccg gcatcaggac ggtgacccgg
     9361 tctgcggcgc gtccgacgcg gcaggcaggc ggcaggcagg cgtcgcccct ccggcaatct
     9421 ccatccgtta gcccgcgttt ctcatcaact gttgatgtca tctgaccggt caccgccaca
     9481 ttccaggcaa cagagcaccg acaaccttca gacgcactgt ctgtctgcgc cctctcgctt
     9541 tccgtgattg tcaacgccgt tcggatgtcg ttaagtggcg ggcgatatgg ccaccgttgc
     9601 cgagcagttg cgcaccgcgc gtgaggcccg ccgcctcacg gtgcagcaga ttgccgatat
     9661 caccaagatg cgggccgatc atgtcgaggc cctcgaagag gggaactaca acgtgttcac
     9721 cgcccccgtc tacatccggg ggttcgtgcg cacctgcgcg aggctcttga agatcgacga
     9781 ggcgtcgatc ctcgagaccc tcgaagtcga gctgacacag acggagaagt tccgcgaaca
     9841 cctgagcctg atcggtcagc agaaaggccc gctcgatgcc ctcatgctga tcctctcccg
     9901 catccggtgg cgcatcgtgc tgcccgcgct gggcgtcctg gtgatcctag ggatcgtgct
     9961 gacgatcgta cgcagcatcg aggagcaaca ggctcgagac ccgctggcgg gcatccagcc
    10021 cgggcagtac cagcccgcgc gatccaatct ggccgaaacc ctcgcgctcc ccaacccggg
    10081 cgccgcgcgg gccccgtccg gcgcgcgatg agcgggcatg aaaaccaggc ccgcccgctc
    10141 agggttggac tgatctctct cggctgcgcc aagaacctgg tggacgccga gatcatgctg
    10201 gggacgctcc tgcgggacgg atacgagatc acgaacgatc tcgctgcctc cgacgccgtc
    10261 atcgtcaata cctgctcctt catcgacgat gcccaggagg agagcgtcga tgccatcctc
    10321 gaatccgccc acatccgtga cgctctgcgg ccaaaccagg cgctcatcgt gtccggctgc
    10381 ctgccgcaac ggttccggga cgatctgccc aaggcgcttc cagaggtgga tgccttcatc
    10441 gggatcgatc aagtcacggc ggtggcccgc atcgtgcggc aagccgtggc gcgtcggact
    10501 gcttggctgc agaaagccga ggacgcccgg gcgcccgttc tgaccgtcag ccgccgtccg
    10561 gtcttcatcc ccgattgcgg cacgccgcga ttcaggctga ccccgcgcca tctggcctac
    10621 gtgaagatcg cggagggctg caatcacgcg tgcagcttct gcatcatccc gcggatgcgt
    10681 gggagccatc ggagccgccc ccaacacgac atcgtcgagg aagccaggcg tctgttggcc
    10741 gagggcgcaa aggaactgaa cctcatttcg caggactcga cctattacgg cctggatttg
    10801 cagcccaagg cggacgtacg gaccatgacc gaccccaagc ggttcggcga tgcaatccgg
    10861 gccttgccgg agcgcgcggc gacgatctgt tccctgttgc gcgccctgaa cgatctgccg
    10921 ggggatttct ggattcggct cctgtacacc cacccggcgc actggaccga cgagctcatc
    10981 gagacgatcg cgcggtgccg caaggtcgcc cgctacgtcg acatccccct ccagcacatc
    11041 cacccggtca tcctcgaaca gatgcggcgc aggacgccgc ccgagcacat caccggtctg
    11101 ctgcgccgca tacgcgccga catcccgggc atcgtcctgc gaacgacgtt catcgtcggc
    11161 ttcccgggcg agacagacgc ccatttcaat accctcctcg agttcatccg cacaaggcgg
    11221 ttcgagcgca tgggggtgtt cgcgtactcg caggaaaagg gcacggtcgc gggccgaatg
    11281 ccgaagcagg tgcctgagtc gatccgacag cggcgccgcg atcaggcgat ggccgtccaa
    11341 aaacagatcg cgcgcgaaca cgcatcttcc cagcttggcc gccgtgtgcg cgtcctcatc
    11401 gagggaacgc tggacgaggc tcgcctcgac gcggtcaggg aaatgccctg gaacaacagc
    11461 ctcgcccgtg tcggcggacg cgccaaggcg tcgtggatgc ggggcgtttg cgcggttggg
    11521 cgcaccgagg cagacgctcc cgaaatcgac ggttgcgtgt acgtaccggg gcaattgccg
    11581 ccgggcgcat tcgtgtcggt gcggatcctg gatcacgcgg actacgacct tctcggggaa
    11641 cctgtttatg agtgagatgc gaacgcaatc gcaggccggc gggacgcccg cgtcggcgga
    11701 gagcgccggc ggcgcctcga tgctcgcgga tttgcgccgg gaaattgacg cgatcgacga
    11761 gcggatcgtc gatttgctcg ggcgccgcca gtccctgacc caaagcgccg tcgccatcaa
    11821 gagggccgcg ggtctgccgg tctatcatcc ggcgcgcgag gagaacctga tctccgagcg
    11881 gcgcgaccag gcgaagcagg ccggtctcga ccccaaccac gtcgaggaac tttatcggtg
    11941 catcctgcgg caatcgcgca tgcgccagac ggtgcgcgcc gccgccaccg gcgtgcggcc
    12001 cggtgcccgc gtcctgctcg tcggcgggcg gggccagatg gggcgctact tcgcgcgctg
    12061 gtttggagag gcgggctacg aaacccggct gctggacgtg gaggactggc cgcgcgctcg
    12121 agatctgtgc gcggagatcg atctggcgct ggtgggcgtg ccgatctctc tcacaacgga
    12181 cgtcatcaaa cgactcggtc cgcatctgcc gccccgctgc attctggccg acatcacaag
    12241 catcaagcgc gcccctgtcg aggccatgat cacggcgcat cccggcccgg tcattggcct
    12301 gcacccgttg ttcggtccat ccacatcgag catggaccag cagattgtcg tggcgactcc
    12361 cggacgcgac gcggacgggt gccaatggct gctcgaccaa ctggccgcct ggggcaacat
    12421 catcatgacc atcgcggccg aggagcacga cgccgtcatg gacctggtcc aggggctccg
    12481 gcatttcgcc acgttcgcct tcggacggtt cctagcggcg cgccaggtca accttgcccg
    12541 aacgctcgag ttctccagcc cgatctaccg cctggaaatg ggaatggtcg ggcgactctt
    12601 cgcccaggac cccggcttgt acgccgacat catcctggca tcgcctcaac ggcgcgcctt
    12661 gctcaaggag ttcatcgagt cggtcggggc agcccgggac ctcatcgaca atgcggatgc
    12721 cgaccgcttt tgccgggagt tccggcaaat cagcgaatgg ttcggcccct tctgcggtca
    12781 ggccctgcgc gagagcacgt ttctgatcga taaactggtc gaacgtttct gagcctgttg
    12841 cccgacggaa cctctaatgg gtgacccatt cacggccttc agggcttggg gtgaaccagc
    12901 agccagtccc ccaaagccgg gcggtgcgat cggcggaagc agtggcgatc cagttttcat
    12961 cggggctgaa ccgcgccatc acgaccggcc cttcgttggg cagggcggcg aacgcccgcg
    13021 ggccggacgt tacttcgaat acagagacgg cacgccgttc ctatggatgg gagacacatg
    13081 gtggaactgg accaagccgg gcattcagtt cagcagcttc cagatttatc tgcgatacgg
    13141 aggcaggctg aaggtgggcc tgcagcccag cgcggcttcg tcgctgtttg actacatctg
    13201 aatcgacttg gaacatgaca gagagggaac acgcggccaa gtgaatggcg actcggcacg
    13261 cgagtttcac gcccctgagg actatcccgg caatctgcag gtcaaggact ggctcccgca
    13321 catccggcgc acggacccta acctggttaa gcgcctgcct gtcttgtgcc ccttcaaggc
    13381 ttggtcgcgc ccgaacatca cgctcgaacg cagggcacgc ggtccgagtt cgcactcgac
    13441 agccatcctt catctgagga gcattcaccg tgacatga
//
LOCUS       DAOQRO010000043        13444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3654_length_13444_cov_5.623123, whole genome shotgun sequence.
ACCESSION   DAOQRO010000043 DAOQRO010000000
VERSION     DAOQRO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_13444_cov_5.623123"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1023
                     /locus_tag="PKK20_02845"
     CDS             109..1023
                     /locus_tag="PKK20_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNU98856.1"
                     /translation="MTEVPATIAPPLSKRATFVRRLSSTVLLWGVVLAALFTPHRAVA
                     DVCFIGLILLLGGAGLIEFYGLIEKRGLVCFKRWGTAGGVLLLGGTFACLSGWRGPQN
                     VLGQANDFETGFLALFVLGLCVRQLVSKSNPAGLTAISTTLFGLMYVGWLLSFVQRIH
                     FYPEANGRLFLIYFVLVSKCSDTGAYVVGSLIGRHKMIPRISPGKTWEGFAGALLAST
                     VASVVFVMLAQGRLVGMNSGHAVILGVLLGVSAVVGDLIESLLKREAGVKDSGRLFPG
                     IGGILDLLDSILFNAPLMYFYLRHVLAN"
     gene            1081..2214
                     /locus_tag="PKK20_02850"
     CDS             1081..2214
                     /locus_tag="PKK20_02850"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNU98857.1"
                     /translation="MKNVVVLGSTGSIGTSTIKVAEDLPDHVRLVGLAAGNNTELLVE
                     QSRKFQPEAVSIGNPDRAAALREMLGASSRVCAGAEGLIELATLPSADIVLIAIVGTA
                     GLQPALAAIRAGKDIAIASKEILVMAGEIVMREAQRSGVRVLAVDSEHSAITQCLGDN
                     PMSSVRRLWLTASGGPFRNTPRSEFDRITMAQALRHPSWVMGTKITIDSATLFNKGLE
                     MIEARWLFGLEMDQVQVVVHPQSVVHSMVEFVDGSILAQLSTPDMCLPIQYALTYPDR
                     VPSGRVRTNLAQIGSLTFQEPDSDRFPAMALARHAGEVGGTLPAVLNAANEVAVALFC
                     EGRLTFPQISAHVAAVMDRHEVVAHPTLDRILEADAWARRQTA"
     gene            complement(2392..5346)
                     /locus_tag="PKK20_02855"
     CDS             complement(2392..5346)
                     /locus_tag="PKK20_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNU98858.1"
                     /translation="MKTVLSLFLSCVSCVPWFLSLVPWFLSALAADPLTESFQKGLLE
                     EDVNRDLKAAAQAYESAVKQADLQRRTAATALFRLAEVYRKLGRTNEAAAQYQRVLLD
                     FADQTNLVSLARQGLIASTGSAASTAASESSASALTPAARRQMELLEQELALVEQQLK
                     QKELLVESGRISRDELLTLQREALSLKRQMAALESQGPLLDIVLPAAESTAAPTPAVS
                     TGIAPTIDPADAELLRQELKLVEQELEVARKKLENGKADAAEVRRVQREALRLQRKLP
                     ENALIDRQIALLQEEFKLTEDSLTEVLKKIQVGVAPPLDEIAARRELLGVQRELAAAE
                     RAATALRQPEASERPAARSAAGAAPATNEEAAEIQRIQAIIKNSPDLVNARNATPVGG
                     TPLHSAAANGYLAVAEFLLANQADVKATDNEGDTPLHTAAAAGHKRFCELLLAKGAEV
                     NAPNKNSYTALHHAVLGGYRAVAETLIAAGAEVNAKGYVPPLERQPRQGQPNWNEMES
                     TPLHLAADKGYPAVAELLAQHGADLNARDRGGRTPLVRAILRRQAAVARLLLEKKADP
                     NVLDKEGSSALLQAVLQKQESLVELLLDHQANVEARLRAPDPEGDWTVLFRTVWDGEV
                     ALTRRLLDAGADVNARAASGITPVHWAVIRDQPAVLELLLERKADVNACDSGDNTPLH
                     YALSLGQPNAEMIEALLVAGAYPNALGWGGTWPPLACVAACDANSHPATLAVILLEHG
                     AAVNGSHTNGWTALHQAAQSNRPDLAELFVANKAKVDARGSRPSQEPKLGERQAQPSA
                     RPTSTAWKAAAPTRAVQTAQPGIPIAPQLIQIPTRAIRSAQPAPPALPAPFQTLVNLG
                     DKTLSADNDVTPLHVAVAIQNLEIARFLLEHGADVNARDSEGRTPLHFAVNRRDLDLM
                     RLLLEAKAEPNAKDNAGQTPLALATSIQDLNQWYTGVSTPDRGTVIMAKPNEILALLR
                     DGDRIEVPDKAE"
     gene            complement(5383..6072)
                     /locus_tag="PKK20_02860"
     CDS             complement(5383..6072)
                     /locus_tag="PKK20_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU98859.1"
                     /translation="MSRILIVEDELAMRTALADVLEDEGYRVITAADGAAGLERAIKE
                     KPDLVLLDIMMPKLDGFAVCAELRRLGHTEPILMLTAKGQVEDRVTGLDVGADDYLVK
                     PFSTDELMARVRAALRRTQRQAKTVRELQLGATRIDLLLQRAWRGTAELHLTAKEFAM
                     LRLLAEAEGEPITRERFLDLVWGYGAFPTTRTVDNHIASLRAKLEPDPAQPRWIKTVH
                     GVGYRLELAEP"
     gene            complement(6057..8282)
                     /locus_tag="PKK20_02865"
     CDS             complement(6057..8282)
                     /locus_tag="PKK20_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNU98860.1"
                     /translation="MSLTETLLAAEERSPGRRRFARRASFVWQAALIILPVVILTMLG
                     LLSLRQDRLLAEQAARERAGRLAQEVADRVFVTLFESDPTPPTNRTTVSPGIDLDRPG
                     FEITPQLELVWPRPVDSPPRPRLLNTTELSEASRAAWESAQHATLDGDTAAVSKGWED
                     FLASEPPAEFAACAQFEWAQTTARSGLTNEAADRFEQLAAAYPDATLESGLPLAPLAA
                     WKAAELRLALATNSTDRITAVLRACSNLVFQPSALSPDLLRRVEELASHLSDQGLQPA
                     QREDGGSDLRVWRGRDSILAWRQLWETTELSRGLFGAARDAWETRGLIHATEAGGIEF
                     SGRAAAPIWVELPAPPPTRMPGQDCASTSWLLTRFPPDSLRLHGWTECQVTNLLARAQ
                     SRLAEWPSYFGLAVTIAGRALPAPSTLPLLAERAAGGPVAPGLDQPAVSAAVYLADAA
                     ALYAHQRQRTWWFVAVVLAAAATAGIGLLAAHRAFRRQLRLSEMKSNFVSSVSHELRA
                     PIASVRLLAESLDRGRITEETKRREYFRLIGQECRRLSALVENVLDFSRIDLGRKQYE
                     FEPTDLIALVTQTVKLMEPYATERQVTLRFEPPGVFNLQPSLDGRAIQQALVNLIDNA
                     VKHSPAGAEVTVTLLAPTTDAPTTDAPTTDAPTTDHGSLMTLSVTDQGPGIPAAEHAR
                     IFEPFYRRGSELRRETSGVGIGLTIVKHVVDAHSGVVRVESELSKGSRFVIELPVCRA
                     S"
     gene            8726..10081
                     /locus_tag="PKK20_02870"
     CDS             8726..10081
                     /locus_tag="PKK20_02870"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98861.1"
                     /translation="MNTASSVPRRAFIQKAATITAIGFAAPHLASAADPTPSASPRPN
                     SRIHVGLIGCGNMGRVNLSNCAVHPDVVVTAVCDVDENRLEPIVDRFKGCRGYRDYRE
                     MLERKDLDAVIIGTLPHWHALQAIAACEAGKDIYIQKPMTLHLAESLAVRNAVRKHAR
                     ISQVGTQHHAREEHHRVIEYVRSGNLGPIGVARAFNVMNQTPRGIGSGGQQSVPEGMD
                     WDLWCGPGPLAPYNRLLAMDAYHHCSWMDFSGGWTPGNGPHMLDLPITALDLGYPLVT
                     SASGGRFVVRDDGDAYDHHEVLWRYPNLTLTWMMSLTNSYGFDLQGDPKPRTRLGVYF
                     HGANGTFYADYLGASTLVPEGEALKGKPVPPMSIPPSPGQEREWLDSIKSRQQPSCSV
                     FHHVKVDVPIVLSLLSLQLGRSIRFDPDTERIVADDEAARRAIPEYRAPWKFPSDYLT
                     A"
     gene            complement(10094..11164)
                     /locus_tag="PKK20_02875"
     CDS             complement(10094..11164)
                     /locus_tag="PKK20_02875"
                     /inference="COORDINATES: protein motif:HMM:NF020340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1793 domain-containing protein"
                     /protein_id="HNU98862.1"
                     /translation="MFGHLPRNANLALKAVIGIGGFARLCELAGKPDDAEKYRAIAQD
                     YAVRWQDLAKGDGRTILAYGQPDTWAMKHNLIWDRVLGLNLFPQSIGDAEIAWYLKVQ
                     KTYGLPVDNRTDTSLIDWALWSIALARNHADFQALLEPIWRYANETPARVPLPDWFVT
                     SDAKRRGFQARPVVGGIFIKMLADSAIRQKWAKRGANTSGPWAPIPVGGEAKEVVPTA
                     RTAQVNWRYTLEEPPADWTEPGFDDSAWKEGPGGFGTQGTPGAIIGTEWNTTAIWLRR
                     EFTLPDRPLKNPRLLILYDEAPEVYLNGVLASGSIRSMSPSGRGIRSPACEAARGDSR
                     GQERGREARHSWFNGFAADSIH"
     gene            complement(11144..11533)
                     /locus_tag="PKK20_02880"
                     /pseudo
     CDS             complement(11144..11533)
                     /locus_tag="PKK20_02880"
                     /inference="COORDINATES: protein motif:HMM:NF027658.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
     gene            complement(11552..12295)
                     /locus_tag="PKK20_02885"
     CDS             complement(11552..12295)
                     /locus_tag="PKK20_02885"
                     /inference="COORDINATES: protein motif:HMM:NF028477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5127 domain-containing protein"
                     /protein_id="HNU98863.1"
                     /translation="MDMVDRPAWVEDLLRKLDLFRTRFTGQGFCLPLHRLASALLMSL
                     AVSTFASDHLRPPAVPIVTHDPYFSIWSPADQLTDADTIHWTGKPRRLTSLVRIDGKA
                     FRLMGTSPTHVPPLPQTWQRVNPTQTHYVFAGEGVVVALDFMTPALPEDTDLLARPVG
                     YVTWHIESTDGKPHAVQLYFDAAAEIVVNEPRQEVVWAREDIGDLVAVKVRSNDQPIL
                     EKKGDDLRIDWGCLYVAAERTSAEPVVAS"
     gene            12656..13402
                     /gene="ruvC"
                     /locus_tag="PKK20_02890"
     CDS             12656..13402
                     /gene="ruvC"
                     /locus_tag="PKK20_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNU98864.1"
                     /translation="MAVQENDVAPLGAGAFEVRSAVGLQLAADSRRRTSTATPPFWQA
                     LAVGVSRRQFEQMQDRLATGRRAAEQMPASTPSTRPNPPQVILGIDPSLRGTGYGVIR
                     TARPGPEALALGVIRCPAAWQRSRCLARIATVLREVIARHQPDACAIEGLFFAQNLQT
                     ALLMGEARGASLLAAAESGLEIYEIAPRKVKQAIVGYGAAGKPAVAKMVQRLLHLTEL
                     PPPDAADALALALTLAQEHGRFSLTPLQRV"
BASE COUNT         2199 a         4198 c         4565 g         2482 t
ORIGIN      
        1 tctccgatct cccatctccg atctccgatc tcccatctcc gatctccgat ctccgatctc
       61 cgatcgcgcg ctcgacacaa agcgacgaca tcggttatcc cttggccgat gaccgaggtc
      121 ccagcaacga tcgcgccgcc cttgtcgaaa agggccacgt ttgtgcgccg gctctcgagt
      181 acggttctct tgtggggggt agtgctcgca gccctcttta cgccgcatcg ggctgtagcc
      241 gacgtctgtt ttatcggcct gatcctgctg ttaggagggg cggggttgat cgagttttac
      301 gggttgatcg agaagcgcgg gctggtctgt ttcaagcgtt gggggactgc agggggggtg
      361 ctgctgctcg gcggcacatt cgcttgcctc tcgggttggc gggggccgca gaacgtcctc
      421 gggcaggcga acgacttcga gaccgggttc ctggccctgt tcgtcctggg cctgtgcgtg
      481 cgtcagttgg tgtccaaatc caatcctgca gggctgacgg cgatttcgac gacacttttt
      541 ggcctgatgt atgtggggtg gctgctcagt ttcgtccagc gcatccactt ttatccggaa
      601 gcgaacgggc ggctttttct gatctacttc gtgctggtca gcaagtgcag cgacacggga
      661 gcgtacgttg tggggtcatt gatcggacgc cacaagatga tcccccgcat cagcccgggg
      721 aagacctggg aagggtttgc cggcgccctc ctggcctcga cggtggcgag cgtggtcttt
      781 gtcatgctgg cgcaagggcg gttggtcggc atgaacagcg ggcacgcggt cattctcggc
      841 gtgctcctgg gcgtctcggc cgtcgtcggc gatctgatcg agtctctgtt gaaacgggag
      901 gcgggggtga aggactcggg ccggcttttt ccaggcatcg gaggcatcct ggacctgctg
      961 gacagcatcc tgttcaatgc cccgctgatg tatttttacc ttcggcatgt gcttgcgaac
     1021 tgaagccggg cggctctgga cttgggcaaa ggcgggctgt tacgataagc ggcgccattc
     1081 atgaagaacg tcgttgtctt gggcagcacc ggctcgatcg ggaccagcac catcaaggtg
     1141 gcggaagacc tgccagacca tgtccgattg gtgggtcttg cggcggggaa caataccgaa
     1201 cttctcgtcg agcagagccg caagtttcag cccgaggccg tctcgatcgg caatccggac
     1261 cgggcggccg cgctgcggga gatgctgggg gcatcgagcc gcgtttgcgc cggcgcggaa
     1321 gggctgattg aactggccac cctgccctca gccgacatcg tgctgatcgc gattgtgggc
     1381 acagcggggc tgcaaccggc gctggcggcc attcgcgcgg gcaaggacat cgccattgcc
     1441 tcgaaggaga ttctggtgat ggcgggcgag atcgtgatgc gggaggcgca acgctctgga
     1501 gtccgtgtat tggccgtgga cagcgagcat tcggcgatca cccaatgcct tggagacaac
     1561 ccgatgagtt cggtccggcg tctttggctg actgcctccg gcggtccatt ccgaaacacg
     1621 ccgcgctcgg aattcgatcg aatcacgatg gcacaggcgc tgcggcatcc gtcgtgggta
     1681 atgggaacga agatcaccat cgactcggcg acgttgttca acaaggggct cgagatgatt
     1741 gaagcgcgct ggctcttcgg tctcgagatg gatcaggtgc aggtcgtggt ccatcctcag
     1801 agcgtcgtgc attcgatggt cgagttcgtc gacggctcga tcctcgctca actctcgacc
     1861 ccggacatgt gtttgccgat tcagtacgca ctgacgtatc cggaccgggt gcccagcggg
     1921 cgcgtgcgga cgaatctggc gcagatcgga agcctgactt ttcaggagcc ggattcggac
     1981 cgattcccgg ccatggcgtt ggcgcgccat gccggcgagg tgggcggcac gttgccggcg
     2041 gtgctcaacg cggccaatga agtcgcggtg gcgcttttct gtgaagggcg tctaacgttc
     2101 ccgcagatca gcgcgcacgt ggcggccgtg atggaccggc acgaggtcgt ggcccacccg
     2161 accctcgatc ggattctcga ggcggatgcc tgggccagaa gacagacagc ctgagcacag
     2221 cgatccgagg tcttggacgg tggccttctc cagtttgtcc gattcggcga attgcgcctc
     2281 caactcaccg atcaggcgcg gaatcttcct cccgaacggt tcgccctcgg cccgaaccga
     2341 tgaagctgaa cccgttgatc gggcccgggc cgcagccgga gtttcctgag ctcactccgc
     2401 cttgtcggga acttcaatcc ggtcgccgtc gcgcaggagg gcgagaatct cgttgggctt
     2461 ggccatgatg actgtgcccc ggtcgggcgt cgagacgccc gtgtaccact ggttcaggtc
     2521 ctgaatactc gtcgccaggg ccaacggcgt ctgacccgca ttgtctttcg cattcggctc
     2581 ggcttttgcc tcgagaagaa gccgcatcaa gtcgaggtcg cggcggttca cggcgaagtg
     2641 gagcggcgtg cgtccttcgg agtcgcgcgc attcacgtcg gcgccatgtt caagtaggaa
     2701 ccgggcgatc tcgaggttct ggatggccac ggccacatgg agaggcgtca cgtcgttgtc
     2761 ggcggagagg gttttgtcgc ccaggttgac cagcgtctgg aatggcgccg ggagcgcggg
     2821 cggagcgggc tgggcggagc ggatggcgcg ggtgggaatc tggatgagtt ggggggcgat
     2881 cggtatgccg ggctgggcag tctggacggc tcgtgtcgga gccgcggcct tccaggcggt
     2941 cgaggtcgga cgggcagacg gctgggcttg gcgctcgccg agcttcggtt cctgcgacgg
     3001 ccgagatccg cgggcatcga ccttcgcctt gttggccacg aacaactcgg ccaggtcagg
     3061 gcggttggac tgggccgcct ggtgcagcgc cgtccagcca ttggtgtgtg agccgttcac
     3121 ggccgcacca tgttcgagca ggatcacggc gagagtcgcc gggtggctgt tcgcatcgca
     3181 tgcggcgacg cacgcgagcg ggggccaggt gccgccccaa ccgagcgcgt tcgggtatgc
     3241 gccggcaacc aacagagcct cgatcatctc ggcattcggt tggccaaggc tcagggcata
     3301 atgcaggggg gtgttgtccc cgctgtcgca cgcgttcacg tccgctttgc gttcgagcag
     3361 cagttcgagc acggcaggct gatcccggat caccgcccaa tgcacgggcg tgatgccgct
     3421 ggccgctcgg gcattgacgt cagccccggc gtcgagcaac cggcgggtca gggccacttc
     3481 cccgtcccac accgtccgaa acaacaccgt ccaatcgccc tcagggtccg gggcgcgcag
     3541 gcgggcctcg acgtttgcct ggtggtcgag gagcaactcg acaagggact cctgtttctg
     3601 gaggacggct tggagaagcg ctgaactccc ctccttatcc agaacgttcg gatcggcctt
     3661 cttctcgagc aggagtcggg cgacggctgc ctgcctcctg aggatggcac ggaccagcgg
     3721 ggtccgtccg cccctatccc gggcgttgag atcggcgccg tgctgcgcga gcaactcggc
     3781 cactgcggga tagcctttgt cggccgccag gtggaggggc gtgctctcca tttcattcca
     3841 gttgggctgg ccttgccgcg gctgacgttc gagtggtggg acgtaaccct tggcgttcac
     3901 ctctgcccca gccgcaatga gggtttcggc cacggcccgg tagccgccca gcacggcgtg
     3961 atgcaaggct gtgtagctgt tcttgttggg cgcattgacc tcggcaccct tggcgaggag
     4021 gagttcgcag aaccgcttgt gaccggcggc ggctgcggtg tgaagcggcg tgtcgccttc
     4081 gttgtcggta gccttgacgt cggcctggtt cgcgagcaga aactcggcaa cggccagata
     4141 cccgttggcc gcggcgctgt ggagcggagt gccgccgacc ggggtggcgt ttcgggcatt
     4201 caccaggtcc gggctgttct tgatgatggc ctggatgcgc tggatttcag cggcttcttc
     4261 gttggtggcc ggtgcggcgc ccgctgcgct tcgagcggcc gggcgctcgg acgcttcggg
     4321 ctgacggagc gcggttgcgg cccgttccgc cgctgccagt tcccgctgca ccccgagcag
     4381 ttcgcggcgc gcggcgatct catcgagcgg cggcgcgacc cccacctgga ttttcttgag
     4441 gacctcggtg aggctgtctt cggtgagctt gaactcttcc tggagcaagg cgatctggcg
     4501 gtcgatgagg gcgttctcgg gcagcttgcg ctgaagccgc aacgcctcgc gctgcacgcg
     4561 gcgcacctcg gccgcgtcgg ccttgccgtt ctcgagcttc ttgcgcgcca cctcgagctc
     4621 ctgctcgacc agcttgagtt cttgccgcag caattcggca tcggccggat cgatggttgg
     4681 agcgatgccg gtcgagaccg cgggtgtcgg agcggcggtc gactcagccg cgggcagcac
     4741 gatgtccaaa agagggcctt gggactcgag cgcggccatt tgccgtttga gggacaatgc
     4801 ctcgcgttgc agggtgagga gttcatcccg agagattcgg ccgctttcga ccaggagctc
     4861 cttctgcttc aactgttgct cgaccagggc cagctcctgt tcgagcagtt ccatctggcg
     4921 ccgggcggca ggcgtcaggg cgcttgcgga cgattcggac gccgccgtgg acgcagcgga
     4981 accggtcgag gcgatgagcc cctgccgggc cagcgacacg aggttggtct ggtcggcgaa
     5041 gtccaggagc acgcgttggt actgggcggc ggcctcgttg gtgcgtccga gcttgcggta
     5101 gacttcggcg agacggaaca gggctgtggc cgcggtgcgg cgctggagat ccgcctgttt
     5161 gacggcgctt tcgtaggcct gggccgcggc cttgagatcc cggttcacgt cctcttcgag
     5221 caggcccttc tggaacgact cggtcaatgg gtcggcggcc agggcggaga ggaaccacgg
     5281 aaccagggaa aggaaccacg gaacacacga aacacacgaa aggaacagcg agaggaccgt
     5341 tttcatgcgg tgaggtttat caaaggtcaa ggcggcgagt tgtcacggtt ctgcaagttc
     5401 gaggcgatag cccacaccgt ggacggtctt gatccagcgc ggttgggccg ggtccggttc
     5461 gagcttggcg cggaggctgg cgatgtggtt gtccaccgtg cgcgtggtgg ggaaggcgcc
     5521 gtaaccccag accaaatcga ggaaacgctc gcgtgtgatc ggttcgccct cggcttcggc
     5581 cagcaggcgc aacattgcga actctttggc ggtgagatgg agttccgcgg tgccgcgcca
     5641 ggcgcgctgg aggaggagat cgattcgcgt cgcgccgagt tgcagctcac gcacggtttt
     5701 ggcctgacgc tgcgtgcggc gcagcgcggc ccggacccgg gccatgagtt catcggtgct
     5761 gaacggcttg accaggtagt cgtcggcgcc aacgtcgagg ccggtgacgc ggtcctcgac
     5821 ctgtcctttc gccgtgagca tgaggattgg ctcggtgtgc cccaggcggc gcagttcggc
     5881 gcacacggcg aagccgtcga gtttgggcat catgatgtcc aggagcacga ggtcgggctt
     5941 ctccttgatc gcccgctcga ggcccgccgc gccgtccgcg gcggtgatga cccgatagcc
     6001 ttcgtcttcg agcacatcgg ccaaagcggt gcgcatggcc agctcgtctt cgacaatcag
     6061 gatgcgcgac atactgggag ttcgatcaca aaacggctgc ctttgctcaa ctcgctttca
     6121 acacgcacca cgccgctgtg ggcgtcgacg acgtgcttca caatggtcag cccgattcca
     6181 accccgctcg tctcgcggcg caactcggac ccgcggcggt agaacggctc gaaaatgcgc
     6241 gcgtgttcgg cggcgggaat gccggggcct tggtcggtca cggagagagt catcagtgaa
     6301 ccgtggtcag tggtcggtgc gtcagtggtc ggtgcgtcag tggtcggtgc gtcagtggtc
     6361 ggtgccagga gggtcacggt gacttcagcg ccggctggcg agtgcttgac ggcgttgtcg
     6421 atcaggttga ccagcgcctg ctggattgcc cgaccgtcca gcgaaggttg caggttaaaa
     6481 accccaggtg gttcgaagcg cagcgtcacc tgccgttcgg tggcgtacgg ctccatcagc
     6541 ttcaccgttt gggtgaccag ggcaatcagg tccgtcggct cgaactcgta ttgcttgcgc
     6601 ccgaggtcga tgcgcgaaaa atcgagcaca ttctcgacca gggccgacag gcgccgacat
     6661 tcctggccaa tcagccggaa gtactcgcgc cgcttggttt cctcggtgat ccggccccga
     6721 tccaggctct cggcgaggag gcgcacggag gcgatgggcg cgcgcagttc gtgcgacaca
     6781 ctcgacacga agttcgactt catctcgctg agccggagtt ggcgtcggaa ggcgcgatgg
     6841 gcggcgagca ggccgatccc cgccgttgcc gcggcggcca gcaccaccgc cacgaaccac
     6901 caggtgcgct gccgctggtg ggcatacaga gccgcggcgt ccgcgaggta cacggcggcg
     6961 ctgaccgccg gttgatcgag gccgggtgca accgggccac ccgcagcccg ctccgccaac
     7021 aacggcaggg tcgacggcgc cggcagcgcg cggccggcga tggtgacggc cagaccgaaa
     7081 tacgatggcc attccgccag tcggctctgg gcgcgggcca gcagattcgt cacctggcac
     7141 tcggtccaac cgtggaggcg gaggctgtca ggagggaacc gcgtgaggag ccaggacgtg
     7201 ctcgcgcagt cctggccggg catgcgtgtg ggcgggggag ccggcaactc aacccagatc
     7261 ggcgcggcag ctcgtccgga gaactcgatg ccgcctgctt cggtggcatg gatcaggccg
     7321 cgcgtttccc aggcgtcgcg cgcagcgccg aacagtccgc gcgagagttc cgtggtttcc
     7381 cagagttgcc gccaggcgag aatcgagtcg cgccctcgcc acacccgcag atccgaaccg
     7441 ccgtcctccc gctgcgcggg ctgaaggccc tggtcactca ggtggcttgc gagttcttcg
     7501 acccggcgga gcaggtccgg actcagcgcg ctgggctgaa agaccaggtt cgagcaggcg
     7561 cgcagcaccg cggtgatccg gtcggtcgag ttcgtcgcca aggccagcct caactccgcc
     7621 gccttccatg ccgccaaggg cgcaagcggc aaaccgcttt cgagcgtggc gtcggggtaa
     7681 gcggcggcca gttgctcgaa gcggtccgcg gcttcattgg tcagtcctga ccgagccgtg
     7741 gtctgcgccc actcgaactg cgcgcaggcc gcaaactccg ccggaggttc ggaggcgagg
     7801 aaatcctccc accccttcga cacggcagcg gtgtctccgt cgagcgtcgc gtgctgggcg
     7861 gattcccagg cggcgcgcga ggcctcggac agttcggtgg tgttgagcag gcgcgggcgc
     7921 gggggcgaat ccaccggtcg cggccagacc agttcgagtt gcggcgtgat ctcgaagccc
     7981 ggacggtcga ggtcgattcc gggactcacg gttgtccggt tggttggcgg cgttggatcg
     8041 gactcgaaga gcgtcacgaa aacccggtcg gcgacttcct gggcgagtcg tcctgcgcgt
     8101 tcgcgggcgg cctgctcggc cagcaggcgg tcttgacgga gagagagcag gccgagcata
     8161 gtcaggatca ccacagggag gatgatgagc gccgcctgcc agacaaacga ggctctgcga
     8221 gcgaaccgac gccgaccggg cgagcgctct tcggcagcaa gaagcgtctc agtcaagctc
     8281 atggttgtcg tcccgcggca tgatgtccga tttcactggt aacagattct gcctcgacag
     8341 ccgagccgat ttgtcacgga aatgcaaagc tgccgtgctc gaacttcgcg gacgacggtt
     8401 cactgttcac cgagttgcgg ctcctccgaa ccgtcgccgc gcagaccgag ggcggaatcg
     8461 agtgcgatcc gcgggctggc cgcgggcatt gaggcttgcg gtgagaacag ccgaacgcga
     8521 gactgccggc cgatgcgacg cccatgcgcc atccatgcac agcgtcagaa gtcccgccgc
     8581 gtgggggcac gcggcgcgca ggcttgcggg tcgggtcccc tgcctgcctc ggcgctgcat
     8641 tctttatgtc gctctgtcga actgatggcg taggctccac gcacagcctg atgaactcga
     8701 gcattcacaa gccaagcacc ctgctatgaa caccgcctcc tccgttccgc gccgcgcctt
     8761 tatccagaag gccgccacca tcactgccat cggctttgcc gcaccccatt tggcctcggc
     8821 ggccgatccg acgccctcgg ccagtccccg tccaaacagc cggattcacg tcggtctgat
     8881 tggctgcggc aacatggggc gtgtgaatct gagcaactgc gccgtgcatc cggacgtggt
     8941 ggtgaccgcg gtctgtgatg tggacgagaa ccggctcgaa ccgattgtcg atcggttcaa
     9001 gggatgccgc gggtaccggg actaccgcga gatgctcgag cggaaggatc tcgacgcggt
     9061 gatcatcggc acgctcccgc actggcatgc gctccaggcc atcgccgcct gcgaggcggg
     9121 gaaggacatc tacattcaga agccgatgac cttgcacctg gccgagagcc tggcggtgcg
     9181 caacgccgtc aggaaacacg cgcgcatcag ccaggtgggc acccagcacc acgcccggga
     9241 agaacatcac cgtgtcatcg aatatgtccg gtccgggaac ctcggaccga tcggcgtggc
     9301 gcgcgcgttc aacgtgatga accagacgcc ccgcgggatc gggtctgggg gccagcagag
     9361 cgtcccggag gggatggact gggatctgtg gtgcggaccc gggccgcttg cgccttacaa
     9421 ccggctgctg gccatggatg cgtatcacca ctgttcctgg atggatttca gcggcggttg
     9481 gactcccggc aacgggccgc acatgctcga tcttcccatc acggccctgg acttaggtta
     9541 cccgctggtg accagcgcct ccggcggtcg tttcgtggtg cgcgacgacg gcgacgccta
     9601 cgatcatcac gaggtgcttt ggcgctaccc caacctcacg ctcacctgga tgatgtcgct
     9661 cacaaacagt tatggttttg atctgcaagg cgaccccaag ccgcggacgc gccttggcgt
     9721 ctacttccac ggagccaacg ggacgttcta cgccgattac ctcggcgcct cgacactggt
     9781 gcccgagggt gaagccttga agggcaagcc cgttccgcca atgtccatcc ctccttcccc
     9841 cggccaggaa cgcgagtggc tcgactcgat caagtcgcgt cagcagccca gttgcagcgt
     9901 gttccaccac gtcaaggtgg atgtgccgat cgtgctcagc ctgctctccc tgcagttggg
     9961 ccgctcgatc cgcttcgacc cggacaccga gcgcatcgtt gcggacgacg aagcggcccg
    10021 tcgggcgatt ccggaatacc gcgcgccgtg gaagttccca agcgactacc tgacggcatg
    10081 aacgcggatg tcctcaatgg atggaatctg ctgcgaaccc gttgaaccat gaatgcctcg
    10141 cttcgcgccc tcgctcttgc cctctgctgt cgcctcttgc cgcctcgcac gccggcgaac
    10201 ggataccgcg cccggatggc gacattgacc tgatcgaacc gctggcgagc acgccattca
    10261 gataaacctc aggggcctcg tcgtagagga tgagcagacg cgggttcttc agaggacggt
    10321 cgggcagggt gaactcgcgg cgcagccaga tcgctgtcgt gttccattcg gtgccgatga
    10381 tggcgccggg cgtgccctgg gtgccgaacc cgcccgggcc ttccttccag gcggagtcgt
    10441 cgaaaccggg ttctgtccaa tcggcgggcg gttcctcgag cgtgtagcgc caattcactt
    10501 gcgcggtccg ggcggtcggg acgacttcct tcgcctcgcc gccaaccgga atcggcgccc
    10561 acggaccgct ggtgttggcc ccgcgcttcg cccacttctg ccggatcgcg gaatcggcga
    10621 gcatcttgat gaagatgcca ccgactaccg gacgcgcctg gaagcctcgc ctctttgcgt
    10681 ccgaggtgac gaaccaatcc ggcagcggca cgcgcgccgg cgtctcgttg gcgtatcgcc
    10741 agatgggctc gagcaacgcc tggaagtccg cgtgattgcg cgccagggcg atgctccaga
    10801 gcgcccaatc gatgaggctg gtgtcggtgc ggttgtcgac gggcaggccg tatgtcttct
    10861 gcaccttgag ataccaggca atctcggcgt cgccgatgga ctgcgggaag aggttcagcc
    10921 cgagcacgcg gtcccagatc aggttgtgct tcatcgccca agtgtcgggc tggccgtagg
    10981 cgaggatcgt ccgtccgtca cccttggcga ggtcctgcca cctgacggcg tagtcctgag
    11041 cgatggcgcg atacttctct gcgtcgtcgg gcttgcccgc caattcgcag agtcgcgcga
    11101 agccgccgat gccgatgacg gccttgaggg cgaggttcgc gttgcgcggc aaatgcccga
    11161 acatgtcggc ggagcaaagc tggtttgtcg gatcgagccc ttccctgacc aggtaatccg
    11221 cccaccggtt cagcatgggc cagtatggtt tggcgaagtc ggcgctgcct tcgtgcctgg
    11281 ccagcgcggc gagcatgatg agcatgttgc cgctttcctc gaccggcatg cggtcaccgt
    11341 ccttgccacc catgccatag acctggccgg tggcgtgcgg gtacgtgccg agatcatgcg
    11401 gcgcgtaagg atagggccag cgcggcgacg cggcataatc gaggatcggc accagcattg
    11461 ccttggtgag cgcggggctg aagacgagga agaacggcgc ctggggaaag aggacgtcga
    11521 ccgtgccgat gcagccgttc gagaaattct cctacgaggc aacaacgggt tccgcactgg
    11581 tcctctcagc cgcgacatag agacagcccc agtcgatgcg aagatcgtct ccctttttct
    11641 caagaatcgg ttgatcattg gacctcacct tgacggcgac cagatcgccg atgtcttccc
    11701 gcgcccaaac gacttcttgc cgaggctcgt tgacaacaat ctcggccgcg gcgtcgaagt
    11761 acagttggac cgcgtggggc ttgccatcag tcgattcgat gtgccaagtc acgtagccga
    11821 ccggtcgcgc gagcaagtcg gtatcctccg gcagggctgg tgtcatgaaa tcaagcgcga
    11881 cgacgactcc ctccccggca aatacgtaat gtgtttgtgt tgggttgacc cgctgccagg
    11941 tctggggcag cgggggcacg tgggtcgggc tcgtgcccat cagccgaaac gctttcccat
    12001 caatccgcac gaggctcgtc agccgacgcg gcttgccggt ccagtggatc gtatccgcgt
    12061 cggtcagttg gtccgccggc gaccagatgc tgaaatacgg atcgtgcgtc acgatcggca
    12121 cggccggcgg acgcaagtga tcggaggcga atgttgaaac ggcaagagac atcaacagcg
    12181 cactcgcaag acggtgaaga ggcaggcaga atccttgacc ggtgaaacgt gttctaaaca
    12241 agtcaagttt ccgtaacagg tcttccaccc aagcgggccg gtccaccatg tccatcagca
    12301 ggagatcgaa tccccgcagc gaccatgccc gctcgaagag cgcaaaacca agattcggag
    12361 ccgcgaaacg ctcctcgccg tcggcggcga ctatcttttg cgcctctgcg agcaggcccg
    12421 gcgcatgcgc tctttcgggg gtcagtctgt ccgccatgca gtcagtacgg ttcacgtttc
    12481 gagcgtcttc aatcccgcct atccacgcag acgcggcaac attggacaag cgttttcggc
    12541 gcgattgccc ggcgctgttc ggagccgcac ccggaatcgc gggatatcct caccaccaga
    12601 agtccggctc gccggtggtg cggacgacta tcctggggaa tctgcaccat tcggtgtggc
    12661 cgtccaggaa aacgatgttg cccccctggg ggcgggcgcc ttcgaggtgc gaagtgcggt
    12721 tgggcttcaa ctggccgcgg attccagacg ccggacctcg acagcaaccc cgccattctg
    12781 gcaggctctc gccgtgggcg tcagcagacg gcaattcgaa cagatgcagg accggttggc
    12841 tacgggccgt cgagcggctg agcagatgcc cgcgtcaacg ccctccactc gaccgaatcc
    12901 gccccaggtc atcctgggga tcgatccttc tctgcgcggg accgggtacg gggtgattcg
    12961 cacggcgcgt ccgggaccgg aagcgcttgc gctgggagtg atccgatgcc cggccgcgtg
    13021 gcaacgctcg cgttgtctgg cgcggattgc cacggtcctg cgcgaggtga tcgcgcggca
    13081 tcagccggat gcgtgtgcga tcgagggctt gttcttcgcc cagaacctcc agaccgcgct
    13141 gctcatgggc gaggcgcgag gggcgagcct tctggcggcg gccgagagcg ggctcgaaat
    13201 ctacgagatt gcgccgcgca aggtgaagca ggcgattgtc ggttacggcg ccgccggcaa
    13261 gccggccgtt gccaagatgg tgcagcgcct gctccatctg accgaacttc cgccacccga
    13321 tgcggccgat gccctggccc tggcgttgac gctggctcag gagcacgggc ggttttcact
    13381 gaccccactg caacgcgtat gatcgccttt ctgcatggca ttctcaccga gagcctcccg
    13441 acgc
//
LOCUS       DAOQRO010000044        13317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3705_length_13317_cov_5.035817, whole genome shotgun sequence.
ACCESSION   DAOQRO010000044 DAOQRO010000000
VERSION     DAOQRO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3705_length_13317_cov_5.035817"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1482
                     /locus_tag="PKK20_02895"
     CDS             376..1482
                     /locus_tag="PKK20_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU98865.1"
                     /translation="MPKTDEQTVFMTSRDRVLSALRRQPIDRVPRLLYEEVIGYTPPI
                     DRLLRTRCESRSPLETFAMDITRVTFAPTALPRSRFADWLGAAAPEALGRGEVDEWGV
                     WRRGGDFHHFVQIESPLCGTLDAQRLDDYPWPDLDQPHRFSDVPRQAANLHARGLAVA
                     AYAGSVFERAWSLRGFTELMMDLVTTPDLAHCLLDRTAAYQRCAAAEFARAGADIIIT
                     GDDVAGQQGLLLRRETWREFLKPRLAATIAAVKRVNPDAFVFYHSDGDVTALIPELIE
                     IGVDILNPIQPECMDPAAVKRAFGDRLALWGAVSVQRTMPFGTPEQVRAEVRERIRTL
                     GAGGGFILSPAHVLSPEVPWENIVAFFDAANEPP"
     gene            complement(1563..2171)
                     /locus_tag="PKK20_02900"
     CDS             complement(1563..2171)
                     /locus_tag="PKK20_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU98866.1"
                     /translation="MAVQPDPDAALMLRVKRGDREAFEQLVEKHKQSVLNLVYRTLPD
                     PTEAEDLAQNVFIQVYRSAHRYRASARFTTWLYTIARNLSLNEIRRRMRHPTESLDQS
                     HPDRSDEPRRQFADDRHGRPDESCLHEELTVKLEAALRDLPENQRIAILLCRDQQLSY
                     EEIAEVLNCSLSATKSLIHRGRETLKQRLKEYLRSGAWEESP"
     gene            2529..3245
                     /locus_tag="PKK20_02905"
     CDS             2529..3245
                     /locus_tag="PKK20_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNU98867.1"
                     /translation="MYELDLTLATPAENLACDEVLLDACNQGGPEVLRLWEPADRFVV
                     LGYSNRFLTEVDSAACQRWRVSVLRRISGGGAVVQMPGCLNYALVLRLDEDTQRQTIG
                     ATNQFVLGRIAKVLQPLIGADVRIEGDTDLALGSRKFAGNAQRRLGQALLFHGCILLK
                     ADIDWMDRLLPLPSRQPAYRAGRRHHAFVTNLGLAPGVVKEALRSAWGAMKPLVEWPR
                     SQVEPLVRTKYTDDSWIRKF"
     gene            complement(3251..4408)
                     /locus_tag="PKK20_02910"
     CDS             complement(3251..4408)
                     /locus_tag="PKK20_02910"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNU98868.1"
                     /translation="MKKPLDAVVVGSGPCGALAARSLAARGWRVALVDRHAFPRQKVC
                     GDALLPDAFDILRDQGLADAVVGAGHPSSVLSVFSPGGYEVRVSGDFLTVRREVLDVL
                     LHRAAIQLGAEPIVAAVSSVSENPDGVCVEFRSGPTLQARACILATGADTRLLRSLQP
                     VISPAPTAVASRCYVQSSRPLDRLVVTYDRGLGPAYAWIFPVGEGLFNVGVGSVYRSQ
                     KRFDHRSLFERFTTVFPEARALMATGQIVSPLRSAPLRCGLDRHALRPGRLFLGAGEA
                     AGSTFPFTGEGIGKAMATGLLAADAVHAFLGGEGEGLDSYASAVLRLEPRYRAYSAAH
                     RLLDYPRLMDLAAWRLQKSHWLAGRLSGVLQEEEDPRSLFSIAGLLGSFFR"
     gene            complement(4850..5974)
                     /locus_tag="PKK20_02915"
     CDS             complement(4850..5974)
                     /locus_tag="PKK20_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU98869.1"
                     /translation="MTDKFFLCYFETTRDCNLACKYCMSRPETPVSAPELSTEEARTL
                     VLDELAKVSSNMAVAFSGGECLLRDDIYTLLAHTASRGMYSFVNTNGKMLVQTDAVRR
                     CRDATGGRVIFVLPFNSIDAESNRSSRDDDTSTVMQATEVCRREGTDYFFLLTISKEN
                     LDTLAPTVQFLKMNRVPMLRAPFVPRGSGMGYVDLFFDADDMKRVIHPALTSSPLSAI
                     SYTPFFADPDALEATWKTYGVKIEGLGCQAGRSFAAVGAEGNVAPCVQLLDSTAVVGN
                     VRQTPLSELIARHPTFQALRQRRENLKGRCGICRYRESCGGCRALAHYRSGDLFAEDP
                     TCFFEPKDRQSRCDLEAMQTVQVEKFVEYLKYNKPWNVLF"
     gene            complement(5977..6501)
                     /locus_tag="PKK20_02920"
     CDS             complement(5977..6501)
                     /locus_tag="PKK20_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNU98870.1"
                     /translation="MGDKAEKLNRFLETLKQESKQEGVSMPELCKAIVSRKNTENAAA
                     FSKAPYAQRLLFMPQCLRVLGKCRAEERAYEYLCARCGSCAAGAIYAKAAALGYGAVK
                     MLKGGSAVARLLDEYTPRAVLGVACAFEGAIGMLECERRGLPVQCVLLQRDGCAETLV
                     DLTEVFETMEFIQP"
     gene            complement(6494..7024)
                     /locus_tag="PKK20_02925"
     CDS             complement(6494..7024)
                     /locus_tag="PKK20_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU98871.1"
                     /translation="MHEEHRRGDFLISTDPGRLDVAAVHAFLSGTYWARGVAIEIVAR
                     SLRNSLSFGLYLGERQVGLARVITDRATFGYVADVYILEPYRGLGLAKWLMTCVVNHP
                     DLQTVRRLLLATRDAHPLYAAFGFRPLRAPNDHLELRPPGAIPGECEAVASQSAGDRW
                     SNRCTCDQTEGNTNNG"
     gene            complement(7104..7514)
                     /gene="atpC"
                     /locus_tag="PKK20_02930"
     CDS             complement(7104..7514)
                     /gene="atpC"
                     /locus_tag="PKK20_02930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNU98872.1"
                     /translation="MATLKLEIVTPEGVAASEDVDMVTLPGVEGEMGIYPQHVPLVTE
                     LVPGEIAVRRGGQEYYLAVGPGFVEITGERVAVLTDLALKADDLDEAKAEEARQAAEA
                     RLKERLSEADALVMNAAVMRSIESLRGRRHARKE"
     gene            complement(7618..9024)
                     /gene="atpD"
                     /locus_tag="PKK20_02935"
     CDS             complement(7618..9024)
                     /gene="atpD"
                     /locus_tag="PKK20_02935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNU98873.1"
                     /translation="MNKGKIVQVIGPVVDVEFAESLPAIYNALNVDYEMPGHGPVKLT
                     LEVQQHLGDNWVRAVAMSTTEGLKRGCEVLDTGKPISVPVGDGVMGRVIDVTGNPVDE
                     RGPVAADRYNPIHRPAPTLTDQSTTPQVLTTGIKVIDLICPFLKGGKVGAFGGAGVGK
                     TVVIMELINNIAKLHGGVSVFAGVGERTREGNDLYNEMSEAGVINQKDLGKSKIALVY
                     GQMNEPPGARLRVGLSGLAITEFFRDERHQDVLLFIDNIFRFSQAGSEVSALLGRTPS
                     AVGYQPTLAAEMGDLQERITSTKEGSITSFQAVYVPADDLTDPAPATTFAHLDATIVL
                     ERAIAELGIYPAVDPLASTSRALTPEIVGQEHYDVARGVQKVLQRYKDLQDIIAILGM
                     DELSPDDKLTVFRARKIQRFLSQPFSVAEVFTGRAGKQVPVADTVRGFKEILDGKHDD
                     VPEGNFYMKGPIEEIKEA"
     gene            9186..11846
                     /locus_tag="PKK20_02940"
     CDS             9186..11846
                     /locus_tag="PKK20_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNU98874.1"
                     /translation="MKHPSSRAARPIRRLALAFCIAAAAHADNQPFATRGADAAPSDP
                     RPASERRQYAPERTIDIRHLALDVTPDFKRRTIAGRAIITFEPVAPLVRELALDAVNL
                     TITEVTASEPIQGWQNTGQQLVVTFADPVPSNRQSRITVGYWTEPAKGLYFRTPEMGY
                     REGETHLFPQGQAIDARHWYPGHDAPNEKFTSEVTCRVPEGMTVLSNGRRMSETRDAL
                     TGLVAVRWLQDKPHVNYLVALVAGYFSAIEDVYRDIPLSFHTLPSAIGEAPNSFRDTK
                     DMMGFFEQEIGVPYPWARYAQVCVNDFVAGGMENTTLTILTDSTLFPAACEGTQSSQG
                     LVAHELVHMWFGDLVTCKDWSHLWLNEGFATYYEALYDGHKNGRDAFLYNMREKAQTV
                     LAISDDTRPIVHREFGDPGEQFSYLAYPKGAWVTHMLRSQLGEDLFRRCIRTYLERHQ
                     YGSVVTEDLNAAIEDVSGRSFDRFFDQWVYHAHYPELEIRYTWDESARLARLNVRQVQ
                     ALSDRVLLFQFPLPVRFACPSGLTTNHIVTVKEKAEDFSFALPEAPRLVRVDPDSTVL
                     AKVKFEPPPAMLALMLTNTNDVLGRLMAVEHFASRKDNEAVSKLRIALESDPFFGVRL
                     EAARALGAIRTDEVLEALLASANQPDPQVRRQVRENLQAFYRQSVLESTRAALDQENN
                     LDIQAIVIRTLGAYPDPQVLETLLRFLKSRSYENRLADAAIDALRTQDDPASIPILLE
                     VLRDRHAAFTARGLASGLITLAHLARHEEQKQTVREFLIAHLNDRRDHVRRTAIEALG
                     GLGDPKAIPALTPFTRGTADNSERHAAQRALANLRAPARPADDLRALREEILTLQRQN
                     RDLEQSIEELKARVAELPSGKAAGNEPNAH"
     gene            complement(11956..12183)
                     /locus_tag="PKK20_02945"
     CDS             complement(11956..12183)
                     /locus_tag="PKK20_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98875.1"
                     /translation="MIEALEEQRRDPNIADLDFEQRLALLVQRQWLWKSNRTLASGPK
                     PIPKRARCRPSKQAPFAALGPREVVQFTPLT"
     gene            complement(12180..12701)
                     /locus_tag="PKK20_02950"
     CDS             complement(12180..12701)
                     /locus_tag="PKK20_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNU98876.1"
                     /translation="MTTVVVDTNTVISAIYWPQSTARRCLAGLARRWYAVAASAEVFA
                     EYEAVAAEFQPRFPACNSAGALAWLRLKAKWVDPAPLGKRRSRDAKDDPFLGCALAAR
                     AGFVVTRDHDLLTLGKPFGIEMITPARFLMWLQENGRRQKYVTNLPRLPVYPYHSDTF
                     LSTRTAADMGGRA"
     gene            complement(12698..12967)
                     /locus_tag="PKK20_02955"
     CDS             complement(12698..12967)
                     /locus_tag="PKK20_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNU98877.1"
                     /translation="MMTQPLNVNLTHELRRYVKEQVRSGRYQNENEVVRDAIRQMQQR
                     EIEQFDRIFGDYTGAPQGEPTDADQAAIKAAIQRHRGGRQAVPRL"
     gene            complement(13015..>13317)
                     /locus_tag="PKK20_02960"
     CDS             complement(13015..>13317)
                     /locus_tag="PKK20_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98878.1"
                     /translation="CVDPSGRRGLSIRVRREWGLRRQPCGRASGHGGRRCYGVAVVQD
                     GDRHGAQLVGELGQVAQGEPALLSPAHDLLPPLFVVVHGQGDLGMIQGRVDGGHRP"
BASE COUNT         2407 a         4387 c         4199 g         2324 t
ORIGIN      
        1 gcttgaagcc ggattccatg ctcaaaccag ccaacttggc aggcgctctc tccagccacc
       61 acggccgcaa tccctcagaa atccaaaagg aatgatcgtg tctcgacccc gtcgccccct
      121 cgacccctcg acgcagcacg aaaacttgac ttgtctccga gtagtgtcat cggtcaagaa
      181 ttcctggcac ggcggcggtc cgaccagtgg atctgggggt tgtcggcggg aatcgagttt
      241 gcggcgcagg cagggcacgc ggcctggagc gggagtctcg atccgttgca ggcccggtga
      301 cctctccggg cgacagaaca ccgacaacaa cccgatgcac cgtcgtccga cccgcttgga
      361 ccgaatcgga gccgaatgcc gaaaaccgat gaacagactg tcttcatgac ctcccgcgac
      421 cgcgtcctga gcgccttgcg ccgccaaccc atcgaccgcg tgccccgact cctctacgag
      481 gaggtcatcg ggtacactcc gccgatcgac cgattattgc ggacgcgatg cgagtcccga
      541 tcgccgctcg aaaccttcgc catggacatc acgcgggtca cattcgcccc gacagccctg
      601 ccgcgatccc gcttcgcaga ctggctcgga gccgctgcgc cggaggcgct cggccgcgga
      661 gaggtcgacg aatggggcgt ctggcggcgg ggtggagatt tccatcattt cgtccagatc
      721 gaatcaccgc tgtgcggcac tttggacgcc cagcgcctcg acgactatcc gtggcccgac
      781 ctcgatcagc cgcaccggtt ttctgacgtg ccgcgacagg cagccaatct ccatgcccgg
      841 gggctggctg tggcggcgta tgcgggctcc gtgttcgagc gcgcgtggtc attgcgcggc
      901 ttcacggaac tcatgatgga cctggtgacg acgcccgatc tggcgcattg cctgctcgat
      961 cgcaccgcag cgtaccagcg ttgcgccgcc gccgagttcg cgcgcgcggg tgcggatatc
     1021 atcatcactg gcgacgatgt ggcggggcaa cagggtctgc tgctcaggcg cgagacctgg
     1081 cgcgagttcc tcaagccgcg cctggcggcc acaattgccg cggtcaaacg cgtcaatccg
     1141 gatgcctttg tcttctacca ctccgacggc gacgtcacag cgctgattcc ggagctgatc
     1201 gagattgggg tcgacattct gaaccccatt caacccgaat gcatggaccc ggcggcggtg
     1261 aagagggcct tcggggatcg actggctctc tggggggcgg tgagcgtcca acgaacgatg
     1321 ccgttcggca caccggagca agtgcgcgcc gaggtgcgcg agcgcatccg aactctcggc
     1381 gccggcggcg gcttcatcct ctcccctgcg catgttctca gtcccgaggt gccgtgggag
     1441 aacatcgttg ccttcttcga tgcggcaaat gagccgccgt agaacccgaa ccctgcctct
     1501 ggcgaagtcc caccaccctg tgcacagacg gggagaaaca tgcgggctag ttggtctcca
     1561 gttcagggag actcctccca ggcgccggag cgcaggtact ccttgagacg ctgcttgagc
     1621 gtctcgcgtc cgcgatggat gagcgatttc gtggcggaca gggagcaatt gagcacttcg
     1681 gcaatctcct cgtaagagag ttgctgatca cggcagagga ggatggcgat ccgctggttt
     1741 tctgggagat cgcgcaaggc cgcttcgagc ttgacggtca actcctcgtg caaacacgac
     1801 tcgtcgggtc tgccgtgacg gtcatcagca aactgacgcc gcggctcgtc agagcggtcc
     1861 ggatgcgact gatcgagcga ctcggtcgga tgccgcatcc ggcggcggat ttcgttcaag
     1921 ctcaggttgc gggcgatggt gtagagccag gtggtgaatc gcgcggatgc ccgatagcga
     1981 tgagcggatc gatagacctg gatgaacacg ttttgggcga gatcctcggc ctccgtggga
     2041 tcgggcaagg tgcggtagac cagattcagc acggactgtt tgtgcttctc gacaagctgt
     2101 tcgaaagcct cccgatcgcc gcgcttcacg cgcagcatca acgcagcgtc cggatcgggt
     2161 tgcacagcca tcgcgccgga ctctaccaac ccgggggagg gcgcgtccag cgaggcgaat
     2221 gccatcgacg actgccaccg tcgtcgcccg ctggagtttg aacattcttg atgcgtgtcg
     2281 gtgtggcagc aggaccttca tcgcacactt catcgttcgg caggccatgt tctatcagcc
     2341 cgagcccaaa ccacgcagaa tcgatgcgat tggatgcagg agggccatgg cgcgcgccgt
     2401 ctgttcggga tcaggctcat tcgtgcttgt gctaacgacc ggccagacgc ttcatcgtgg
     2461 gtgcgcgaac tcgatcggct ggcacgcggc acgaaaccgg agcgagcctc cgccgagcgc
     2521 cgtttcgaat gtacgaactc gatttgacct tggctacgcc cgccgagaac cttgcctgcg
     2581 acgaggtgct gcttgacgcc tgcaatcagg gcggcccgga ggtgttgcgc ttgtgggaac
     2641 cggcggatcg ctttgtcgtt ctcgggtact cgaaccggtt cctgacggag gtcgactcgg
     2701 ccgcctgcca gcgatggcgc gtctccgtgt tgcgtcggat cagcggcggc ggcgcggtgg
     2761 tgcaaatgcc gggctgcctg aactacgcgc tggtcctgcg tcttgatgag gacacccagc
     2821 ggcagacgat cggggcgacg aatcagttcg tcctgggccg catcgcgaag gtgctgcaac
     2881 cgttgatcgg agctgatgtg cggatcgagg gcgacacaga ccttgccttg gggtcgcgca
     2941 agttcgcggg caacgcccag cggcggctcg gacaagccct gctcttccac ggctgtatcc
     3001 tcctgaaagc ggacatcgac tggatggacc ggttgctccc gctcccttcc cgacaaccgg
     3061 cgtatcgagc cgggcgccgg catcacgctt tcgtgaccaa cctcggactg gcgccgggcg
     3121 tggtgaaaga ggccttgagg tcggcgtggg gcgccatgaa gccgctggtc gagtggcccc
     3181 gaagccaggt cgagcccctg gtcagaacca agtacaccga cgactcctgg attcgaaagt
     3241 tctgaccagt tcaacggaag aacgatccca ataagccggc gatcgagaag agcgatcggg
     3301 ggtcctcctc ttcctgcaag actccgctca accgacccgc cagccaatgg ctcttctgaa
     3361 ggcgccaagc cgcaaggtcc atcaagcgcg ggtagtccag gagccggtgc gcggccgagt
     3421 aagcgcggta tctgggttcg agtcgaagga cggccgacgc gtaggagtcg aggccttctc
     3481 cctcgccccc caggaaggcg tggaccgcgt ccgccgcgag aagaccggtc gccatggcct
     3541 tgccgatgcc ttcgccggtg aaggggaagg tcgagccagc cgcctccccc gcacccagga
     3601 acagtcgccc gggccgcaac gcgtgccgat ccaggccgca acgcaggggg gctgagcgca
     3661 gcggactcac aatctgaccg gtggccatca aggcccgggc ctcggggaac acagtcgtga
     3721 agcgttcgaa gaggctgcgg tggtcgaacc tcttttgcga acgatacacg ctgccgacgc
     3781 cgacattgaa caggccctcg cccacgggaa agatccaggc ataggccggc ccgaggccgc
     3841 ggtcataggt caccacaagc cgatcgagcg gacggcttga ctgcacataa cagcgcgagg
     3901 cgacggcggt gggcgcaggc gaaatcacgg gctgcaagga tcgcaggagg cgcgtgtccg
     3961 ccccggtcgc aaggatgcag gcccgcgcct gcagggtggg accggatcgg aactcgacgc
     4021 agacgccgtc cgggttttcg gagactgagc tgacggcggc cacaatcggc tcggccccga
     4081 gctggatcgc ggcacggtgc agcaggacgt cgagcacctc gcggcggacg gtgagaaagt
     4141 cgccgctgac gcgcacctca tagccgccgg ggctgaacac cgagagcaca ctcgatgggt
     4201 gtcccgcgcc cacgaccgcg tcggccagcc cctggtcgcg cagaatgtcg aaggcatccg
     4261 gcaacagtgc gtcgccacag accttctggc gcgggaacgc gtgtcggtca accagcgcca
     4321 cgcgccagcc gcgcgccgcg agagaccgcg cagcgagcgc gccacagggc cctgagccga
     4381 cgacgacggc gtccagaggc ttcttcatgc tttctggaat ccttgaccgg tgaaactcgc
     4441 tgtagacaag tcaagtttct gtgctgcgtt gaagggggtc aaggattcta agggtctgtg
     4501 caaaagttac ttccggtttt ggcgggagcg ccaccgggcc agatgcgagg cccgagggag
     4561 ggagtgtatc ccctgagata ctcgaccgac cgaggaacga cgcagatgcc cggtgccgcc
     4621 ccgccccgga gggctgggag gctttggctc gctgacttcg tggctcctca gtcacagagg
     4681 tcaaggctat gctccttcgt cgcgcctcgc cagcgagcac aatcccctcc agcaaaaccg
     4741 gaactgattt tgcgcagact ccaaggaacg aggttcggga gcgggggtga ggaaacgagg
     4801 agactcctca tggaaagcag gacttaattc tggactgaag cgcgcctcgt tagaacagga
     4861 cattccacgg tttgttgtac ttgaggtact cgacaaactt ctcgacctgg acggtctgca
     4921 tggcttcgag gtcgcagcgg ctttggcggt ccttcggctc gaagaagcaa gtgggatcct
     4981 cggcgaagag atcgccgctc cgataatggg ccaaggcgcg gcagccgccg caactctccc
     5041 ggtaccggca gatgccgcag cgtcctttga ggttttcgcg ccgctgccgc agggcctgga
     5101 aggtggggtg gcgcgcgatc aactcggaca gcggcgtctg gcgcacgttg ccgaccaccg
     5161 cggtcgagtc aaggagctga acacatgggg ccacgttgcc ttcggcgccc accgcggcga
     5221 acgatcgccc ggcctggcaa cccagcccct cgatcttgac gccataggtt ttccaggtgg
     5281 cttcgagcgc gtcgggatcg gcaaagaacg gcgtatagga aatggccgag agcgggctgg
     5341 acgtcagggc ggggtggatg acgcgcttca tgtcatcggc gtcgaagaac aaatccacat
     5401 accccatgcc cgagccacgc ggcacgaagg gggcgcgcag catcgggacg cggttcatct
     5461 tcaggaactg caccgtcgga gccagcgtat cgaggttctc cttcgagatc gtcagcagga
     5521 agaagtaatc cgtcccctcg cgccggcaga cctcggtggc ctgcatcacc gtcgaggtgt
     5581 cgtcgtcgcg gctgctgcgg ttggactcgg cgtcgatcga gttgaagggc agcacgaaga
     5641 tgacgcggcc cccggtggcg tcccggcacc ggcggactgc atcggtctgc acgagcatct
     5701 tgccgttggt attgacgaag ctgtacatcc cccgggacgc tgtgtgagcc aggagagtgt
     5761 agatgtcgtc ccgcaggagg cactcgcctc ccgagaaggc caccgccatg ttcgagctca
     5821 ccttggcgag ttcgtccaac acgagcgtcc gcgcctcttc cgtgctcaat tccggagccg
     5881 acaccggcgt ctcgggccgc gacatgcagt acttgcaggc gagattgcag tcgcgcgtgg
     5941 tctcgaagta gcagaggaaa aacttgtcgg tcatggtcat ggctgaatga actccatggt
     6001 ctcgaagacc tcggtgagat ccacgagggt ctcggcgcat ccgtcccgct ggagcaagac
     6061 gcactgaacc ggcaggccac ggcgctcgca ttccaacatc ccgatcgccc cctcgaaggc
     6121 gcaggcgact cccaacacgg cccggggcgt gtattcatcg agcaagcgcg ccacggcgct
     6181 gccgcctttg agcatcttca cggcgccgta ccccagggcc gccgccttgg cgtaaatggc
     6241 gcccgcggcg caggagccgc accgtgcgca caggtattcg taggcccgct cctcggcgcg
     6301 gcacttgccg agcacacgca ggcattgggg catgaacagg agccgctggg catagggcgc
     6361 tttcgagaag gcggcggcat tctcggtgtt cttgcgcgac acgatggcct tgcagagctc
     6421 gggcatcgat acgccttcct gcttcgactc ttgcttcagc gtttcgagaa agcggttcag
     6481 tttttcggcc ttgtcaccca ttgttcgtgt ttccctccgt ttgatcacat gtgcagcgat
     6541 tgctccatcg atctcccgcg gactgggaag ccacggcttc gcattcgccc gggatcgcgc
     6601 ccggcggacg cagttcgagg tgatcattcg gcgccctaag cggacggaat ccaaaggccg
     6661 cgtacagcgg gtgggcgtcg cgtgtggcca gcagcagccg ccgcacggtc tgcaagtcgg
     6721 ggtgattcac aacacacgtc atcagccact tcgccaggcc gagtcctcga tagggctcga
     6781 gaatgtagac atccgccacg tacccgaacg tggcccggtc cgtgatcaca cgagccagtc
     6841 cgacctgccg ctcgccgagg taaagcccga agctcaggct gttgcgcagg gaacgtgcga
     6901 cgatctcgat cgcgacgccc cgggcccagt atgttcccga caggaaggcg tggacagcgg
     6961 cgacgtccag gcggccggga tcggtgctaa tcaggaagtc gccgcggcgg tgttcttcgt
     7021 gcatcgggaa tggggggctg gcgccgggct tcgagcgcgt gtggccgggc cgttcgcagc
     7081 ccgccggcct cggcgtgacg gggctactcc ttgcgcgcgt ggcggcgccc gcgcagcgac
     7141 tcgatcgacc gcatcaccgc ggcgttcatc acaagcgcgt ccgcctcgct caggcgttcc
     7201 ttgaggcgcg cttccgcggc ctgccgcgcc tcctcggcct tcgcttcgtc cagatcgtcc
     7261 gccttgagcg ccaggtcggt caggaccgcc acccgttcgc ccgtgatctc gacgaagccg
     7321 ggtccgacgg cgagatagta ctcctgtccg ccgcggcgca cagcgatctc gccgggcacc
     7381 agttcggtca ccagcggcac gtgttgtggg tagatgccca tctccccttc gacgcccggc
     7441 agcgtgacca tgtccacatc ttcagatgcg gcgactccct ccggcgtgac gatttcaagt
     7501 ttgagtgtgg ccatcggtcg ctgggtgttc gtcagtcaga catggaagcg ggttatggtt
     7561 acgctccgag gggtcaggca accgcgccag gcgttctggc cggcatcgtt cgtgactcta
     7621 ggcttccttg atctcctcga tcgggccctt catgtagaag ttgccctccg gcacgtcgtc
     7681 gtgtttgcca tcgagaatct ccttgaaacc gcgcacggtg tccgccacag gcacctgctt
     7741 gccggcgcgg ccagtaaaaa cttccgcaac cgagaacggc tgactcagga aacgctggat
     7801 cttccgggcg cgaaagaccg tgagcttgtc gtccggcgac aactcgtcca tgccgaggat
     7861 ggcgatgatg tcctgcagat ccttgtagcg ctggaggacc ttttggacgc cgcgcgcgac
     7921 gtcgtagtgt tcctggccga cgatctccgg cgtcagggcc ctcgaagtcg aggcgagcgg
     7981 gtcaaccgcc ggataaatgc ccagctccgc gatggcccgt tcgagaacga tggtcgcgtc
     8041 cagatgtgcg aaggtcgtgg cgggcgccgg atcggtgagg tcgtccgcgg gcacatacac
     8101 cgcctggaac gatgtgatcg agccctcctt ggtggacgtg atgcgttcct gcagatcgcc
     8161 catctcggcc gcgagcgtgg gctggtatcc gaccgcgctg ggcgttcggc ccaggagggc
     8221 ggacacctcc gagccggcct gcgagaagcg gaagatgttg tcgataaaca gcaacacgtc
     8281 ctgatgccgt tcgtcgcgga agaactccgt gatggccagg ccggagaggc cgacgcgcag
     8341 gcgggcgccg ggcggttcgt tcatctggcc gtaaacgagc gcgatcttcg acttgcccag
     8401 gtctttctgg ttgatcacac ccgcttcgga catttcgttg tagaggtcgt tgccttcgcg
     8461 cgtgcgctcg ccaacgccgg cgaacactga cacaccgccg tgcagcttgg cgatgttgtt
     8521 gatcaactcc atgatcacaa cggtcttgcc aacccccgcc ccgccgaagg cgcccacctt
     8581 gccccccttg agaaacggac agatcaggtc gatcaccttg atcccggtcg tcaacacctg
     8641 tggcgtggtc gactgatcgg tcagcgtggg cgcgggacgg tgaatgggat tgtaccgatc
     8701 ggcggccaca ggcccgcgct cgtcgaccgg attgcccgtc acgtcgataa cgcgccccat
     8761 gacgccgtcg cctacgggca cggagatcgg tttgccggta tcgaggacct cgcagccgcg
     8821 cttgaggcct tcggtcgtgg acatggccac cgcgcgcacc cagttgtcgc ccagatgctg
     8881 ctgcacttcg agcgtcagct tgaccgggcc atggccaggc atctcgtaat cgacgttcag
     8941 ggcattatag atcgctggca gcgactccgc gaactcaacg tccacaaccg ggccaatgac
     9001 ttgaacaatc ttgcctttat tcatgactgc aacgccgtct atctcatccc acgcgggttc
     9061 cccctccccc agcgaatcga gcactcaggg tgcgcgaatc taggggctgt cccgaaccag
     9121 tgtcaacgcg gatattgtcc tccggacttc tctttcttgc gccgggcgtg tagaagcgaa
     9181 agcgcatgaa acatcccagc tcccgggcag cccgtccaat ccggcgcctc gctctcgcct
     9241 tctgcatcgc cgccgccgcc catgccgaca atcagccgtt cgcgacacgc ggcgcggatg
     9301 cggccccgag cgacccgaga ccggcttccg agcgccggca atacgcgccg gaacggacga
     9361 tcgacatccg ccacctggct ttggatgtga cacccgattt caaacgacgg accattgcgg
     9421 gacgcgcgat catcaccttc gagcccgttg cccctctcgt gcgcgaactc gccctggacg
     9481 cggtcaacct gaccatcacc gaagtcaccg cgtcggaacc gatccagggc tggcaaaaca
     9541 cgggacagca gcttgttgtc accttcgcgg accccgtacc gtcgaaccgg caatcgcgca
     9601 tcacggtcgg ttattggacc gaaccggcca aggggcttta cttccgcacg cccgagatgg
     9661 gctacaggga gggggaaaca cacctgttcc cgcagggcca ggcgatcgac gcccgccact
     9721 ggtatccggg ccacgacgcc ccgaatgaga agttcacgtc cgaggtcacc tgccgcgtcc
     9781 ccgagggcat gaccgtgctc tcgaacggca ggaggatgtc cgagacccgg gacgcgctca
     9841 ccggcctggt tgcggtgcgt tggctgcaag acaagccgca tgtaaactat ctggtagcgc
     9901 tggtggccgg ctacttcagc gcgattgaag acgtgtaccg tgacattccg ctgagcttcc
     9961 acaccctccc gtcggccatc ggcgaggcgc ccaactcgtt tcgggacacc aaagacatga
    10021 tgggcttctt cgagcaggag atcggcgtgc cctatccgtg ggcgcgctac gcccaagtgt
    10081 gcgtcaacga cttcgtggcg ggcggcatgg agaacacaac cctcaccatt ctgaccgaca
    10141 gcacattgtt ccccgcggcc tgcgaaggga cgcaatcgag ccagggcctc gtggcgcacg
    10201 agctggtcca catgtggttc ggcgacctgg tgacctgcaa ggactggagt catctctggc
    10261 tcaacgaggg attcgccacg tattacgaag ccctctacga cgggcacaag aacggacgtg
    10321 acgcgttcct ctacaacatg cgcgagaagg cccaaacggt cctggccatc tccgacgaca
    10381 cacggcccat cgtccatcgg gaattcggcg atccgggcga gcagttcagc tacctggcgt
    10441 atcccaaagg cgcctgggtg acgcacatgc tgcggtccca attgggcgag gatctgttcc
    10501 gccgctgcat ccggacctat ctcgaacgcc accagtacgg ctcggtggtg accgaggacc
    10561 tcaacgcggc catcgaggat gtgtcaggac gttcgttcga ccgtttcttt gaccagtggg
    10621 tctaccacgc gcactacccg gaactcgaaa tccgctacac gtgggacgag tctgcccgcc
    10681 tggccaggct caacgtgcgc caggtgcagg ccctcagcga ccgggtcctc ctgttccaat
    10741 tcccgctccc cgtccgcttc gcctgcccgt cgggcctcac caccaatcac atcgtcaccg
    10801 tcaaagagaa ggcggaggac ttctcgttcg cgctgcccga agccccgcgg ctggtccggg
    10861 tggacccgga ttccacggtt ttggcgaaag tgaagttcga gcccccgccg gccatgctgg
    10921 cgctgatgct gaccaacacg aacgacgtcc tgggccggtt gatggccgtc gagcacttcg
    10981 caagccgcaa ggacaacgag gcggtgtcca aattgcgcat cgccctcgaa agcgacccct
    11041 tcttcggcgt ccgtctcgaa gccgcgcgcg ctctgggcgc aatccgcacg gacgaggttc
    11101 tcgaagcact gctggcttca gccaaccaac ccgatcccca ggttcgccgc caggtgcgtg
    11161 agaacctgca ggccttctat cgccagtccg tgctcgagtc cacacgcgcc gcgctcgacc
    11221 aggagaacaa cctcgatatc caggcgatcg tcatccgcac cctgggcgcc tatcccgacc
    11281 cgcaagtcct ggagaccctg ctccgcttcc tcaagtcccg gtcgtatgag aaccggctgg
    11341 ccgatgcggc gattgacgcc ctgcgcaccc aggacgaccc ggcctcgatt ccgatcctgc
    11401 tcgaagtgct ccgcgaccgg cacgcggcgt tcaccgctcg cggccttgcc tcgggcctga
    11461 tcaccctcgc ccatctggcc cgacacgagg agcaaaagca gaccgtccgc gagttcctga
    11521 tcgcccacct caacgaccgt cgggaccacg tgcgccggac ggccatcgag gcgctcggag
    11581 gactcggcga tcccaaggcc atccccgccc tgaccccgtt cacccgcggc accgccgaca
    11641 actccgagcg ccatgcggca cagcgcgccc ttgccaacct gcgcgccccc gcgcggcccg
    11701 ccgacgatct gcgcgcgctg cgggaggaaa tcctcaccct gcaaagacaa aaccgtgacc
    11761 tcgaacaaag catcgaggaa cttaaagcca gagtggccga actcccgtcc ggcaaagccg
    11821 ccggcaatga gccaaacgcg cactgaaaac cgcgggtcga atcttcgatc tcgcgaaccc
    11881 gccctcctcc gccctggtgg ccgttggaac gtatccgtcc ggggaagggc agtaggcgcg
    11941 gcgccctcga tctgctcaag tgagcggggt gaactggacg acctcgcgag gaccgagagc
    12001 ggcgaacgga gcttgcttac ttgggcggca gcgtgcgcgc ttcgggatgg gctttggccc
    12061 actcgccagc gtgcgattgg atttccaaag ccactgtcgt tgcaccagca acgccagacg
    12121 ttgctcgaag tccaggtcgg cgatattggg atcgcggcgt tgttcctcca aggcttcgat
    12181 catgccctcc cccccatatc cgccgctgtt ctcgtgctta aaaaggtgtc actatgatag
    12241 ggataaacag gtagacgggg aaggttcgtg acgtatttct gccggcgtcc attctcctga
    12301 agccacatca ggaagcgcgc cggcgtgatc atctcgatgc caaacggctt gcccaaggtg
    12361 agcaaatcat ggtcccgcgt gaccacgaat ccagcacgcg cggcgagggc gcacccaaga
    12421 aacggatcat ctttcgcatc gcggctccgg cgcttgccta agggagcggg gtcaacccac
    12481 ttcgctttca accggagcca agccaacgcc ccggcgctgt tgcaggcggg aaaacgcggc
    12541 tgaaactccg cggcaacggc ctcgtattcg gcaaacacct ccgcggaagc cgcgaccgca
    12601 taccaacgcc gcgccaagcc cgccaggcac cgccgtgctg tgctttgagg ccagtagatg
    12661 gcgctgatga cggtgttcgt gtcgacaacg accgtagtca tagcctcggc acggcttgcc
    12721 tcccgcctcg gtgacgctga atggcagcct tgatggcggc ttgatctgca tccgtcggct
    12781 cgccctgggg ggcgccggtg taatcgccga agatgcggtc gaactgctcg atctcgcgct
    12841 gctgcatttg ccgaatggca tcgcgcacga cttcgttctc gttctggtac cggcctgacc
    12901 gcacctgttc cttgacatat cgccggagtt catgtgtcag gttcacgttc agtggttgcg
    12961 tcatcatgcc ccgcacaata gccgttctgg ccatatttgg caatcttggc catatcacgg
    13021 gcggtgccct ccatccacgc ggccctggat catgcccaga tcgccctgcc cgtggacgac
    13081 cacgaatagc ggcgggagca ggtcgtgggc tggactgagg agcgccggct cgccctgcgc
    13141 aacctgtccg agctcgccga ccagttgcgc gccatgacgg tcgccatcct ggacgacggc
    13201 aactccgtag catcggcgac ctccgtgacc actggcgcgc ccgcacggct gccgtcgaag
    13261 accccactct cggcggaccc gaattgacaa tccccgccgg cccgaaggat caacgca
//
LOCUS       DAOQRO010000045        13248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3746_length_13248_cov_4.658910, whole genome shotgun sequence.
ACCESSION   DAOQRO010000045 DAOQRO010000000
VERSION     DAOQRO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_13248_cov_4.658910"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PKK20_02965"
     CDS             <1..1237
                     /locus_tag="PKK20_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNU98879.1"
                     /translation="IRPAFAFDPSGGPDGKGCLVITSDAREGLDGHWTKTVPIQGGRH
                     YRFAALRRTENVPTPRRNVLARILWQNGQGRQVLHDEPGALSYRDGPLPVAEPEYPTD
                     KGADATGWMEVSDTYLAPLKATHAVVELHLRWAPGARVEWSRVSLDEVPAPEPRMVRL
                     ATAHFRPSGQKTAADNCRLFAPLIAEAARQKADLLVLPETITICGNGLSFAEAAEPIP
                     GPSTDFFAALAREHNLHLVVGLLERDRHRIFNVAVLIGPDGQILGKYRKVALPRTEIE
                     GGVAPGVDYPVFNTRFGSVGMMICYDGFFPEVARQLSINGAEVIAFPVWGCNPLLAAA
                     RACENHVYIVSSTYTDVATRWMISAVYDHEGRVVAQATEWGAVAVAEVDLNRRLHWSS
                     LGDFKAEIPRHRPVWKGDQ"
     gene            complement(1575..2708)
                     /locus_tag="PKK20_02970"
     CDS             complement(1575..2708)
                     /locus_tag="PKK20_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU98880.1"
                     /translation="MIDRAVAQLLRRRLAQFPAVTLVGPRQCGKTTLARTLAQSYFNL
                     EAPEERTLLDARWEEVVACDGPVVFDEAQHWPQLFPRLQGAIDARRKRNGRFILLGSI
                     APSLMRDVGESLAGRMAVVDLAPFCLGEVDDLERLWRCGGFPDGGILGGEAYPVWQES
                     YLRAMAERDLPSWGLPAKPVVTQRLFRMVAAEQGSILNLSKLGQSLGLSHHTVNAYLD
                     HLEGSFLIRRLLPFAANLRKRLVKAPRLYWRDSGVLHALLRLPSGDDVLAQPWAGASW
                     EGFVIEQILTARAIRGEGCDACFFRTHDGYEADLVIESGRAREVIEIKLTSGPTPEDL
                     ARLAQVGQLIEATQLVLLCRVGRSVTTGRQWVTNLPDYLQAKA"
     gene            complement(2961..3098)
                     /locus_tag="PKK20_02975"
     CDS             complement(2961..3098)
                     /locus_tag="PKK20_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98881.1"
                     /translation="MNEKLMLEHRDTRLLWDVQVPSRLCPDCIKPTFSGRGRLIPNER
                     R"
     gene            3103..4263
                     /locus_tag="PKK20_02980"
     CDS             3103..4263
                     /locus_tag="PKK20_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNU98882.1"
                     /translation="MNRVVEEKAARDAAYRAELKRNGKVLLSEARKLSDDALLSKLAE
                     FGIALDREGLRKLVDKFLSAEAIFREAIKGCRRPIARSKMGEDWVWFAFTLLWERWFP
                     DIPSTEMFDERMQEGYRLQERDLAAACDCWLGVWRDFLTLLERSGVRSIAEFDEELRG
                     TQFVANWVQDLDIALSNASVGRPEYHRQRMRWADEFLQRFGGHDSHMTELIRWALGEA
                     TWGSGERAQAESLFEAWLREDPQWGWGWIAWSDLHWFPRHNARPDHARAEGLLQRGLA
                     VPGVRDREELITRLTDLYKKTGRSDEVAALAQHATKARTTATVAGENQLRLRTTFDFG
                     AEGLPLEAMLQPSAEPRQRHFELLQTVSQRKVGRNEPCPCGSGKKFKHCCGR"
     gene            4979..5626
                     /locus_tag="PKK20_02985"
     CDS             4979..5626
                     /locus_tag="PKK20_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU98883.1"
                     /translation="MKKLIVSFVGGAVAFTVHAQVLFTYEFPGSDPGGDATVPAPEHM
                     TLSAFARTQVNAVNQIDAFSSSHWTQGAEQDPNEFVSFAFRPDVGYEVALSSLSWDTS
                     RSSTGPQIGKVELWRNGVNFESSQEFEVTTTLGRRVFDFTPTTGLSSDSYEFRFYGWN
                     ASGTGNLRLDNVAVVGAITPIPEPSASGLVLAAGLVGMTWRRLHRARRTAFIPTT"
     gene            5785..7095
                     /locus_tag="PKK20_02990"
     CDS             5785..7095
                     /locus_tag="PKK20_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU98884.1"
                     /translation="MLKSRDPNCDRAQSSPETRFHRGPAFVNGALLLLSALCVVSSLA
                     AVNWPELRGPLRNGHALDANPPLSWSETNRIVWKTAIHDLGWSSPVVWGEQIWLTTAT
                     TDGREMYAVCVDAQTGRVVMDVKVFDTPEPEHISNLNSYASPTSAIEAGRVYVHYGTY
                     GTACLDTENGKVLWSRRDLNCDHHEGPGASIMLDGDRLYVPVDGRDVQYVVALDKATG
                     RTVWRTDRSVDYSQFPSNCRKAFCTPIAIETGGRRQLFSPAAKGMFAYDPDTGAELWK
                     ARYNGWSMVPRPLFGHGLLYVITDYERPELWAVRPDGQGDVTDTHVAWKVVKDMPSTA
                     SLLLIDDLLYMVNDQGFALCVEAKTGAVVWRERIGGKHSASPIYTPGRIYFFNERNRA
                     TVIAPGREFRVLGGSQLDDPLMASPAVTGDALILRSRTHLYRIQ"
     gene            7109..13108
                     /locus_tag="PKK20_02995"
     CDS             7109..13108
                     /locus_tag="PKK20_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNU98885.1"
                     /translation="MQLDRPRSGAAPALHTGSRVARLIALVWAGILASALSSLGAVQL
                     DLAQLGMATQTGDLSAANAPARALDGNAGTFSATTEIPNAYWECELSRVYTLTRIEIV
                     APSDSSYGDALNGLVLRLEDLRDCTVFETPVNDPGPGSTWAADLPAGLDARILRIGLE
                     NGQPNGAGKHQVALAEVRAFGDPSVATGPIALGAIATARQSSDFSPSYPASLAIDGDP
                     ATFSQTADLTNSFWTLTLDRVRPIQRIEVVNRRDTTPARLAGLVVRILDDQSNTVAST
                     TLTNPGLGGVWGFDPPAGASGRHVRIGLEGGAANGAGDRIVSLAEVIVLTAPNLAQGR
                     DCYMVRYQDNLPPATNANDGNYATETKTTDASVDAYWEVDLGVPHALYHVRAVAAEGF
                     AARLSHATVRLFDAGHDSVFSKHLAGTNPTFDVDVAGPITARYVRVGFENKERSHITG
                     GIEWHLGLREVQVFGRPADEIGLLAFNASTNRIALGDSTTLTWQSEELHRLDLYPGGR
                     SVGAFTDQGGAGQLTVSPANSVEYLLVGTNRNGASVRAVSVIVDDRPLPVRISEFMAE
                     NRLSLEDGRGEPSDWIELHNPNNQPCDLAGYGLSDDPATPMKWLFPSVSIPAHGYLVV
                     FASGRNAPWDEAGGLHANFQLDAEGESVVLTAQDGASAVDVISAFPPQREDLAYGRTL
                     EGRLAFLDPTPGGPNVAASYDGWLQPPAFSHARGFHTNAFALTITDPNQGGELLYSID
                     GREPALRYGAPLAVSTSLSVRATVRRPGYKSPRTVTHSYVLIDADMASSSMNQNMVRD
                     ARYTDCIRRGLTDLPALMIAVPQIPDDYIEREASVELLWPDRTPHVQANCGFFRFGGA
                     WTTFSKKNYRLKFRGEYGATKLRAPLFRGMDRGFLARESFDEIDLVGGSHDMIDRGFY
                     MAGRFTEDTMLDMGSLNPHGRFVNVYLNGKYWGQYHARERLTDSFLADYLGGEREDYV
                     NVRGNDNVGDSFIPGTPDPRNRAPWENLRAHRSSYIASKPSLDVPHLIDFMLMWFYGD
                     CETEYRAAGPIAPGSGFKFWIGDADGFLRTSALTSDRTSNPGPGGLFGALVSEKHPDF
                     MTLLADRIQKHFFLDGALTPARNLARLDERMLEITNSLVAECARWGVRTPENWQSAAQ
                     TIRTGLFPGRTTNLFNLLRNRGLYPSLKAPEMNQWGGTVTNGFTVLFAGTGGTIYYTL
                     DGSDPRLPGGAVAPGALVAGNGGEETLIAPGSEWRYWDKGAPPAATWNSPSFNDQSWS
                     SGRSELGYGDGDETTVLQFGPDSNNKYPAYYFRRAFTASDPAAITELVVDLLRDDGAI
                     VYLNGKELFRDNMPAGVVEYADLASTAVGGADESRFFSFATPPTGLVAGTNLLAVEVH
                     QSGGASTDVSFNLALHARSRTEATGIVIDRNTSIRARALSGTTWSALVEARFDIAEPQ
                     PVIPGQIVISEVHYNPAGSPDCEFIEFWNISSNLVDLTGARLAGGVDFLFPSGFRIPP
                     GGFALVVENTAAFAARYQSPDSPYSYPDLNVAGAWVGRLDNAGESVALLTPQLDPVCA
                     VTYRPDGSWPRRADGQGSSIELRDPASVPTDATAQTHYLAQGRHWRASPLYHGSPGRL
                     DAWVQPIVINESWAGGDRTTDWIELHNPGAEPFDLCGLYLSDRYDQPFRHAFQQELHV
                     PAEGYLVLSAAEFGFGLGTRGSDILLTSASGTNIVRFIDTVDFPAVDRKETSGRYERS
                     DGMIDYAELRAATPGEANALPRVGPVVFSEIMYHPAPGLAEYVELVNISDAAIDLCDP
                     AVPTSVWKLTGAVRFAFSPGQSLAPWTPLIVCNTNAAAFRTQYAVSDKTPVLGPWTGL
                     LDNAGEDLRLVRPGPPGTDGAVPCYRVDRVEYEPRTPWPLIPDTGGASLERMTLEGYG
                     NDPGNWIASPIGGTPGRLQANQLPLRIRCVFDHAERIPRFFVEGSPDATYEIQYTDTL
                     TPSVWRLLKTIEDAGPGPIEFRDPQFPLPHHRFYRVVRVGE"
BASE COUNT         2464 a         4551 c         3945 g         2288 t
ORIGIN      
        1 aatccggccc gcattcgcct tcgatccctc cggcggcccc gatggcaaag gctgccttgt
       61 gatcacatcg gacgcgcgcg agggcctcga tggtcattgg accaaaaccg tcccgatcca
      121 gggcggacgg cactaccgct tcgccgcgct gcgccgcacg gagaacgtgc cgacgccccg
      181 ccgcaatgtg cttgcgcgca ttctctggca gaacggccag gggcgccagg tcctccacga
      241 tgaacccgga gccctcagct accgcgacgg acccctgccc gtggccgagc cggagtaccc
      301 gaccgacaaa ggcgcggacg ccacggggtg gatggaagtc tcggacacct accttgcacc
      361 gcttaaggca acccatgcag tggtcgaact ccatttgcgc tgggcccccg gcgccagggt
      421 cgagtggagc cgcgtctccc tcgatgaagt gccggccccg gagccccgaa tggtccgcct
      481 cgccactgcc catttccgcc cgtccggcca gaagaccgcg gcggacaact gccgcctgtt
      541 cgccccgctg attgcggaag ccgctcgcca gaaggcggac ctgctggtgt tgcctgagac
      601 catcaccatt tgcggcaatg gccttagctt tgctgaagcc gccgaaccga tccccggccc
      661 ctcgacggac ttctttgccg ccctcgcccg cgagcacaat ctgcacctgg tggtgggctt
      721 gctggaacgc gatcggcatc ggattttcaa cgtggccgtt ttgatcggcc ctgatggaca
      781 gattctgggc aagtaccgca aggtcgccct gccgcgaacc gagatcgaag gtggagtcgc
      841 ccccggcgtc gactacccgg tgttcaacac ccggttcggc agcgtcggaa tgatgatctg
      901 ctacgacggt ttcttccccg aggtggcgcg tcagctcagc atcaacggtg ccgaagtcat
      961 tgcctttccc gtttggggct gcaacccgct gctcgccgct gcgcgcgctt gcgagaacca
     1021 cgtctacatc gtcagcagca cctacacgga cgtggcgacc cgctggatga tctcggccgt
     1081 ctatgaccac gaaggacgcg tcgtcgccca agccacggaa tggggcgccg tcgccgtcgc
     1141 ggaggtggac ctgaaccggc ggctgcattg gtcgagcctg ggcgatttca aagccgagat
     1201 tccccgtcat cgcccggtct ggaagggaga tcaatgattt catggctgca caatcaggac
     1261 gaaacatgaa ggaaatcgca aggagtcatc cgccattcat gatccgcccc gcttcatgag
     1321 gcgcttttcc ctccgtgtgc tctgcgcctc tgtggtgaat cccatccgat gactgtcacc
     1381 gccccggatt gaaccacaga gacacagagc gcgcagaggg agggccaatc aactccaagc
     1441 ggaagcgccc gatcctggtg gcctgtctct ggggtcactg caacaccgcg cgatagaagc
     1501 cgtagggcgc actgaggttc catcgatcaa taccaaggac cgacctgtct ggaaaaccaa
     1561 agacaaacct cggctcaggc cttcgcttgc aggtagtcag gaaggttcgt cacccactgg
     1621 cgaccggtcg tgacggaccg gccgacgcgg cacagcagca ccagttgggt ggcctcgatc
     1681 agttgcccaa cctgagccag ccgagccagg tcctccggag tcggtcccga cgtcagcttg
     1741 atttcgatca cctcgcgcgc tcgcccgctt tctatcacga ggtcggcttc gtaaccgtcg
     1801 tgggtgcgaa agaaacaggc atcgcacccc tcgccgcgga tggcgcgcgc cgtcagaatc
     1861 tgctcgatca caaagccttc ccaactggca ccagcccaag gctgggccag cacgtcgtcg
     1921 ccagagggca accgcagcag cgcatggagc actccagaat cgcgccaata gagacggggc
     1981 gccttgacca gccgtttgcg caggttcgcc gcgaacggca gcaaccgacg aatcaagaag
     2041 gagccttcca aatggtcgag gtaggcgttg accgtgtggt gcgagaggcc gaggctctgg
     2101 cccagcttcg acaagttcag gatcgatccc tgttcggccg ccaccatgcg gaagagccgt
     2161 tgcgtgacca caggtttggc gggcagcccc cacgaaggca ggtcgcgctc cgccatggca
     2221 cgcaggtagc tctcctgcca tacgggatag gcctcaccgc caagtattcc gccgtcggga
     2281 aagccgccgc agcgccagag tcgctccagg tcatccacct cacccaggca gaatggcgcc
     2341 agatcaacga ccgccatgcg ccccgccagc gactcgccaa catcccgcat cagcgaaggc
     2401 gcgatggagc ccaggaggat gaagcgcccg tttcgcttgc gccgcgcgtc aatcgcgccc
     2461 tgtaaccgcg ggaacaactg cggccagtgt tgggcttcat cgaacaccac cggtccatcg
     2521 caggcgacga cttcctccca ccgggcatcg aggagagtcc gttcttccgg tgcttccaga
     2581 ttgaagtaac tctgcgccag cgtgcgggca agcgtcgtct taccgcactg gcgcggcccg
     2641 acaagcgtga ccgcgggaaa ctgcgccagc cgtcgtcgga gcaactgcgc cacggctcgg
     2701 tcaatcatgt cttgaaaata ggaaaccgat ttccaatttt caagacaaca cccaaaacaa
     2761 atcgaccgca caggcgcgaa caccgatgca gacgccacca tgggcatgta cgccttgtcc
     2821 gctctcgcct ttgtgtgctc cgcgcctctg aggtgaattc cgtccgatga ctgtccggtt
     2881 cgggcccagc agggtcggcc tctcgggagc agcgcagggc tgccgtccct ggcgacggcc
     2941 cctcgcctcg aacaggttct tcaacgacgt tcgttgggga tgagccgtcc ccgccccgaa
     3001 aaggtgggct tgatgcagtc cgggcaaagc cggcttggga cttgaacgtc ccacaggagg
     3061 cgtgtatctc ggtgctcgag catgagcttc tcattcatcg caatgaatcg ggtcgtcgag
     3121 gaaaaggcag cgcgcgacgc ggcgtatcgg gccgaactca aacgcaacgg caaggtcctg
     3181 ttgtcggagg ctcgcaagct gtccgacgac gccctgttgt ccaaactcgc cgaattcggc
     3241 attgcgcttg accgggaggg attgcggaag ttggtggaca agtttctctc cgccgaggcc
     3301 atctttcgcg aggcgattaa ggggtgtcgc cgcccgatcg cccggtccaa gatgggcgag
     3361 gattgggtct ggttcgcctt cacattgctg tgggaaaggt ggtttcccga catccccagc
     3421 acggagatgt tcgacgaacg catgcaggag gggtatcggc tccaggagcg ggaccttgcg
     3481 gccgcgtgcg attgctggtt gggcgtctgg cgcgatttcc tgactctcct cgagcggagc
     3541 ggcgtgcgct caatcgccga gttcgacgag gagttgcgcg ggactcagtt cgtcgcgaat
     3601 tgggtgcagg acctggacat tgcgctgagc aatgcctcgg tgggccgtcc ggagtaccat
     3661 cgccaacgca tgcgctgggc agacgagttc ctgcagcgct tcggcgggca cgactcgcac
     3721 atgaccgagc tcatccgctg ggcactgggg gaggcgacat ggggcagcgg tgagcgcgca
     3781 caggccgaga gcctcttcga ggcctggctg agggaggacc cgcaatgggg ctggggctgg
     3841 atcgcgtggt cggacctgca ctggtttccg cgccacaatg ccaggcccga tcatgcgcga
     3901 gccgagggac tccttcagcg cgggttggcg gtgcccggcg tgcgcgatcg cgaggagctg
     3961 atcacccggc tgaccgacct ctacaagaag actggccggt ccgacgaggt tgctgccttg
     4021 gcacaacatg cgacaaaggc ccggacgacc gcaactgtcg cgggcgaaaa ccaacttcgc
     4081 ctgaggacga cgttcgactt cggcgcagag gggctgccgt tggaggcgat gctccaaccc
     4141 agcgccgaac cgcgccagcg gcatttcgag ctcctgcaaa cggtgtccca gcggaaggtg
     4201 gggcgcaacg agccttgccc gtgcggtagc ggcaagaaat tcaagcattg ctgcggacgc
     4261 tgagtcggga attgttcttc tctgcgctct ccgcgcctct gtggttcaat ccggaaccgt
     4321 actgtcatcg agggggattc accacagagg cgcagagcac gcggagaaaa ccggctgaag
     4381 ccgagcctgc aatcgcgaaa cccgacaatg ggtcaccgat ccgcggaaga tgcccgatag
     4441 ctcgctcgcc tccaggtctc tgcctgtcct caagcgggac accctttcga ctcccggctc
     4501 aacaacagga cacccgccga ccagcccgga tgcggttcag cgcggagccc gtgaccgatc
     4561 cgggctcgcc cgcatattta cacaccgatg gtggggaggg cactgagact ccgcaagagg
     4621 actgctatgc gggctagaga agtaaaaccg ccccagattc gagagacaaa gcaccgcaac
     4681 acactgtttt catggcagac cacccgcagg accgacagaa tgctcccgat tccctttctg
     4741 gacgcggttt agccacagac cggatgtcag actctgcgaa gctctgtgaa acctccgggc
     4801 ttgccggcaa ccctggcacg gcaactgctt gcccgtctcg gcacgaggag gtcaacaccg
     4861 gggtcggacg agcccaacgt cggatcgctt gaagcgggcg ccaggtctcg gccgacagat
     4921 ggaagccctc ctcgccaagg aaaacaccaa atacacatcc gtaaccgtca ggagcaccat
     4981 gaagaaactg atcgtctcat tcgtcggcgg ggctgtggcg ttcacagtcc acgcgcaagt
     5041 gttgttcacc tatgaatttc ccggcagcga tccgggcgga gatgccacgg tcccggcgcc
     5101 ggaacacatg actctgagcg ccttcgcacg gacccaggtc aacgccgtga accaaatcga
     5161 tgccttctcg agttcacact ggacacaggg agcggagcag gatccgaacg aattcgtttc
     5221 gttcgcattt cgcccggacg tcggatacga ggtcgcgctc tcctcgctga gttgggacac
     5281 ctcgcgcagc agcaccggcc cacagattgg caaggtggaa ttgtggcgca acggcgtgaa
     5341 cttcgaaagc tctcaggagt tcgaagtgac caccacgctc ggccgccggg tctttgactt
     5401 cacacccaca acagggttgt cgagcgattc ctacgagttc cgcttctacg gctggaacgc
     5461 aagcggcacg ggcaatctcc gcctcgataa cgtcgccgtc gtcggcgcca tcaccccgat
     5521 cccggaaccg tccgcctcgg gcctggtcct cgccgccggc ctcgtcggaa tgacctggcg
     5581 ccgcctgcac cgcgcccgtc gcaccgcctt cattccgact acctagtccg cttccgaacc
     5641 gtgcccgtca gaggctcgga ccttgcgcgc ccaccaaggc gaggcaaagg cccgagcctt
     5701 ctgcttttgc caaatggtcg cgcactctca tttttcgcac actgaatgct cgctttcccg
     5761 acggcggcat cggatcatac gcccatgctg aagtccagag atccgaactg cgatcgcgct
     5821 caatcgagtc ccgaaaccag attccaccgg ggaccagcct tcgtcaatgg cgcgcttctc
     5881 ctcctgtccg ccctttgcgt cgtgtccagt ctcgccgccg tcaattggcc ggaactgcgc
     5941 ggccccctca gaaacgggca cgccctcgat gcaaacccgc cgttgtcgtg gagtgaaacg
     6001 aaccgcatcg tctggaagac ggccatccac gatctgggct ggtcatcgcc ggtcgtctgg
     6061 ggcgagcaaa tctggctcac gacggccacc accgacgggc gtgagatgta cgccgtgtgc
     6121 gtggatgccc agaccggacg cgtcgtgatg gacgtcaagg ttttcgacac gcccgagccg
     6181 gagcacatat cgaacctcaa cagctacgcg tcgcccactt ccgcaatcga agctggaagg
     6241 gtctatgtcc actacgggac gtatgggact gcatgcctcg acacggaaaa cggcaaggtc
     6301 ctttggagtc gtcgtgactt gaattgcgat catcacgaag gtccgggggc gtcgatcatg
     6361 ctcgatgggg accgcctcta tgtcccggtc gatggccgcg atgttcaata cgtggtcgcc
     6421 ctcgacaagg ccacggggcg caccgtgtgg aggacagacc ggtcggtcga ttacagccag
     6481 tttccgagca attgccgcaa ggcattctgc acacccatcg caatcgagac cggcgggcgc
     6541 cgccaactct tcagcccggc cgccaagggc atgttcgcct acgatcccga cactggcgcc
     6601 gaactctgga aggcgcgcta caatggctgg tccatggtcc cgcgcccgct tttcggacac
     6661 ggattgctat atgtcatcac cgattatgaa cgcccggaac tctgggccgt gcggcccgac
     6721 ggacaaggcg acgtcacgga cacccatgtc gcgtggaagg tggtcaaaga catgccttcc
     6781 acagcctcgc tcctgttgat cgacgacctg ctctacatgg tcaatgacca gggattcgcc
     6841 ctctgcgtcg aggccaaaac cggcgccgtg gtctggcgcg aacgcatcgg cggaaaacac
     6901 tccgcgtcgc ccatctacac ccctggccgc atctacttct tcaacgagag aaaccgcgcg
     6961 accgttatcg cgcccggacg cgagttccgc gtgctgggag gaagccagct cgatgatcca
     7021 ctcatggcga gtcccgccgt caccggagac gccttgatcc tcagaagcag gactcacctc
     7081 taccgaatcc aatagacgga gttctgccat gcaattggac cgcccaaggt ccggcgcggc
     7141 ccccgcactg cacacgggtt cccgagttgc tcgcttaatc gccctggtct gggcaggcat
     7201 tctcgcctcc gccctgtcgt ctctgggcgc agtccagttg gacctggctc agctgggcat
     7261 ggccacccag accggcgacc tgtccgccgc caatgcgccg gcccgcgcgc tcgatggaaa
     7321 cgcaggcacc ttcagcgcaa caaccgagat tcccaatgcg tactgggagt gcgaactgag
     7381 ccgggtctac accctgaccc gcatcgaaat cgtcgccccg tcagactcct cctacggcga
     7441 tgccctcaac ggcctcgtgc tccggctcga ggaccttcgc gattgcactg tcttcgagac
     7501 gccggtcaac gatcccggtc cggggtcgac ttgggccgcc gacctccccg ccggcctgga
     7561 cgcgcgcatc ctccggatcg gcctcgaaaa cggccagccc aacggagccg gcaaacacca
     7621 ggtcgccttg gccgaggtgc gcgcgttcgg cgacccctcg gtggcgactg gcccgattgc
     7681 gctcggcgcc atcgccacgg cccgacaatc aagcgacttc agcccgtcct atccggccag
     7741 cctggccatc gacggcgatc cggcaacctt ctcccagacc gccgacctca caaacagttt
     7801 ttggaccctc acgctcgacc gcgtccgccc gatccagcgc atcgaggtcg tcaaccggcg
     7861 cgacaccaca ccagcccgac tcgcgggatt ggtcgtgcga atcctcgatg atcaatccaa
     7921 tacagtcgcc tcgaccaccc tgaccaatcc gggtctgggc ggggtctggg gcttcgaccc
     7981 cccggcgggc gcgtcgggtc gtcacgtgcg catcggactc gaaggcggcg ccgccaacgg
     8041 cgccggcgac aggatcgtgt cgctggccga ggtgatcgtc ctgaccgcgc cgaacctggc
     8101 ccagggacgg gactgctaca tggtacggta ccaggacaac ctgccgcccg cgaccaacgc
     8161 gaatgacggc aattacgcca ccgaaaccaa gacgaccgat gcgtccgtcg atgcctattg
     8221 ggaggttgac ctgggcgtgc cccatgccct ctaccacgtc cgcgccgtgg cagccgaggg
     8281 attcgccgcc cgcctcagcc acgcgactgt tcgcctgttc gatgccggcc atgattcggt
     8341 gttctcgaag catctcgccg gcaccaatcc aaccttcgac gtcgatgttg cgggaccaat
     8401 cacagcgcgc tatgtccgtg tcggcttcga gaacaaggaa cggtcgcaca tcaccggcgg
     8461 cattgaatgg cacctgggcc tgcgcgaagt ccaggtcttc ggacgccccg cggacgagat
     8521 cggcctgctc gccttcaatg catccaccaa ccgcatcgcc ttgggcgact cgaccacgtt
     8581 gacctggcag agcgaggaac tgcatcggct cgacctctat cccggcgggc gatcggtcgg
     8641 cgccttcaca gaccagggcg gcgcggggca gctcaccgtc tcacccgcga actccgtcga
     8701 gtatctgttg gtcggcacca accgcaacgg cgcatctgtg cgagcagtca gcgtcatcgt
     8761 cgatgaccgc cctctcccgg tgcggatcag cgagttcatg gccgaaaacc gcctctctct
     8821 cgaagatgga cgcggcgaac cttccgactg gatcgaattg cacaacccca acaaccagcc
     8881 ctgcgacctt gccggctatg gattgagcga cgatccggcc acccccatga agtggctctt
     8941 cccgtccgtc tcgatcccgg cccacggcta ccttgttgtg tttgcgtccg ggcgcaacgc
     9001 gccgtgggac gaggccggcg gactgcacgc gaatttccaa ctcgatgccg agggcgaatc
     9061 ggtggtgctg accgcccagg acggcgcgtc ggcggtcgat gtgatctccg cttttcctcc
     9121 gcagcgcgag gacctggctt atggaagaac gctcgagggg cggctggcgt ttctcgatcc
     9181 cacgccgggc ggccccaatg tggccgcatc gtatgacggt tggttgcagc cgccggcctt
     9241 cagccatgcc cgcggtttcc acaccaacgc cttcgcgctc acgatcacgg acccgaatca
     9301 aggcggcgag ctgctctact cgatcgacgg gcgcgagcct gctctgcgct acggcgcgcc
     9361 tctggcggtg tcgaccagcc tcagcgtgcg cgcgaccgtg cggcgacccg gctacaaatc
     9421 gccgcgcacc gtcacccatt cgtacgtgct catcgatgcc gacatggcgt cctcctcgat
     9481 gaaccaaaac atggtccggg acgcccgcta caccgactgt atccggcgcg ggctgacgga
     9541 tctgcccgcg ctgatgatcg ccgtgccgca gattcccgac gattacatcg agcgcgaggc
     9601 ctcggtcgag ctgctctggc cggaccggac gccgcacgtc caggccaact gcggcttctt
     9661 ccgcttcgga ggcgcctgga ccacgttcag caagaaaaac taccggctca agttccgcgg
     9721 cgagtacggc gcaaccaagc tgagggcgcc cctgttccgc ggcatggacc gcggcttcct
     9781 cgcccgcgag agcttcgacg aaatcgacct ggtcggcgga tcgcacgaca tgatcgatcg
     9841 cggcttctac atggcgggcc gcttcaccga ggacaccatg ctcgatatgg gcagcctcaa
     9901 tccccacggg cgtttcgtga acgtatacct caacggcaag tactggggcc aataccatgc
     9961 gcgcgaacgc ctcaccgaca gcttcctggc cgattacctc ggcggcgaac gcgaggacta
    10021 tgtcaacgtg cgcggcaacg acaacgtcgg cgacagcttc atcccaggca cgcccgatcc
    10081 ccgcaaccgc gccccctggg aaaatctgcg ggcgcaccgc agctcctaca tcgcgtcgaa
    10141 accctctctc gatgtgcctc acctcatcga tttcatgctg atgtggtttt acggcgattg
    10201 cgaaaccgaa taccgcgccg ccggtcccat cgcccccggc agcggtttca aattctggat
    10261 cggcgacgcg gacggtttcc tgcggacctc ggccctcacc tcggatcgaa cctccaaccc
    10321 gggcccgggc ggcctgttcg gcgcgctggt ctccgaaaag catcctgatt tcatgaccct
    10381 gctcgccgat cgcatccaga agcacttctt cctcgatggc gcgttgaccc ccgcgcgcaa
    10441 cctcgcccgc ctcgacgagc gcatgctcga aatcaccaac agcctcgtcg ccgaatgcgc
    10501 gcgctggggc gtccgcacac ccgaaaactg gcaaagcgcc gcccagacaa tccgcaccgg
    10561 cctcttcccc ggccgcacca ccaatctctt caacttgctc cgcaatcgcg gcctctatcc
    10621 ctcactcaaa gcgcccgaaa tgaaccaatg gggcggcacg gtgaccaatg gcttcaccgt
    10681 cctcttcgcc ggaaccggcg gcacaatcta ttacacgctt gacggatcgg acccgcgcct
    10741 gcccggcggc gccgtggcgc ccggagcgct cgtcgccggc aacggcggcg aggagacgct
    10801 catcgcgcca ggctccgaat ggcgttattg ggacaaagga gcgccgccgg cagccacatg
    10861 gaatagcccg tccttcaacg accagagctg gagctccggc aggtccgagt tgggctacgg
    10921 cgacggtgac gaaacaacgg tcctccagtt cggccccgac tcgaacaaca aataccccgc
    10981 ctactacttc cgacgcgcat tcactgcgtc cgaccccgcg gccatcaccg aactggttgt
    11041 cgatctgctc cgcgacgatg gcgcgatcgt ttacctcaac ggcaaggaac tcttccgcga
    11101 caacatgccc gcgggcgttg tcgagtatgc ggacctcgcc tcgacggccg tcggaggcgc
    11161 ggatgagtcg cggttcttca gcttcgcaac gcctccgacg ggcctggtgg ccggcacaaa
    11221 ccttctggcg gtcgaggtcc atcagtccgg tggagccagc accgacgtca gtttcaacct
    11281 ggccctgcac gcgcgctcac gaaccgaggc aaccggcatc gtcatcgatc gcaacacctc
    11341 gatccgggcc cgcgcgctca gcggaaccac ctggagcgcc ctcgtcgagg cccgcttcga
    11401 tatcgccgaa ccgcaaccgg tcatccccgg ccagatcgtg atctccgagg tccattacaa
    11461 cccggccgga tcgcccgact gcgagttcat cgagttctgg aacatcagct cgaacctcgt
    11521 cgacctgacc ggcgcccgcc tggccggtgg cgtcgacttc ctgttcccct caggttttcg
    11581 cataccgccc ggcggcttcg ccctggttgt cgaaaacacc gccgcctttg ccgcacgcta
    11641 tcaatcgccc gattcgccct actcctatcc ggatttgaat gtggccggcg catgggtcgg
    11701 acgactcgat aatgccggag aatccgtcgc gttgctgacg ccccaactcg atccggtttg
    11761 cgcggtgacc taccgtccgg atggctcctg gccccgcagg gccgacggcc agggcagttc
    11821 catcgagctc cgcgatccag cctccgtccc cacagacgcg acggcccaga cgcactacct
    11881 ggcccaagga cgtcattggc gcgcaagccc gctctatcac ggttcccctg gccgcctcga
    11941 tgcctgggtc cagccaatcg tcatcaatga atcctgggcc ggaggtgaca gaaccaccga
    12001 ttggatcgaa ctccataacc cgggcgcgga gccgttcgac ctgtgcggcc tctatctcag
    12061 cgaccgatat gaccagccgt tccgacacgc cttccagcag gaactccatg tcccggcgga
    12121 aggatacctc gttctgtccg ccgccgaatt cggattcgga ctcggcacac gcggatccga
    12181 catccttctc acaagcgcgt cggggacgaa catcgtgcgc ttcatcgaca cggtcgattt
    12241 cccggcggtc gaccgcaagg agacgtccgg gcgttacgag cgttcggacg ggatgatcga
    12301 ttacgccgaa ctgcgcgccg caacgccggg agaagccaat gccctgccgc gcgtcggccc
    12361 cgtcgtcttc agcgagatca tgtaccaccc cgcgcccggc ctggccgagt atgtcgagct
    12421 ggtcaacatc tcggacgcgg caatcgacct gtgcgacccg gctgttccga ccagcgtgtg
    12481 gaaattgacg ggagctgtca gattcgcgtt ttcgcccggc caaagcctgg ccccctggac
    12541 accgctgatc gtctgcaaca caaacgccgc cgccttccga acccaatacg ccgtgagcga
    12601 caaaaccccg gtgctcggcc cctggaccgg cctgctcgac aatgcgggtg aagacctccg
    12661 actcgtccgg cccgggcccc ccggaaccga tggcgcagtg ccctgctatc gcgtggaccg
    12721 tgtcgagtac gagccgcgaa cgccgtggcc tctcattcca gacaccggcg gcgccagcct
    12781 cgaacggatg acgctcgaag gatatggcaa tgaccctggg aattggattg cctctcccat
    12841 cggaggcacg ccgggtcggc ttcaggccaa tcaactgcct ctccgcatcc gatgcgtctt
    12901 cgaccacgcc gagagaatcc ctcggttctt tgtcgagggt agtcccgacg ccacgtacga
    12961 gatccaatac acggacactc ttaccccgag tgtctggcgc ctccttaaga caatcgaaga
    13021 tgcgggcccc ggcccgatcg aattccgaga cccccagttc ccgctgccgc atcaccgctt
    13081 ctaccgcgtc gtgagagtgg gagagtagcg tcgatggggt cggtcccgaa cggcagttag
    13141 ttaaggcctc gcagagttgc ggtatttgcc ggcatgggga ctgttcttgt tctcgggggt
    13201 tttgcagctc aagtgatcca tctggagggt gactttgagg tggcgcat
//
LOCUS       DAOQRO010000046        13220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3762_length_13220_cov_4.636536, whole genome shotgun sequence.
ACCESSION   DAOQRO010000046 DAOQRO010000000
VERSION     DAOQRO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3762_length_13220_cov_4.636536"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /locus_tag="PKK20_03000"
     CDS             complement(<1..1893)
                     /locus_tag="PKK20_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNU98886.1"
                     /translation="MNRPRGKAWIWTARLAAALLLGSPNRTGASPPTAPNSLTEPKIK
                     PARLIVRGHGFLGNLRLKQQLRVLQPDSRPPEFLDSRILEDAALLLLSALQREGYLEP
                     AIELQVLCDDGTESVLSARPEAPLEAPAHWRARRAVFLLDRGAFHYYARLDFTGLTAL
                     SKAKAAGCFLVPDMVVPQKKPKVYTPARLDRGLASLREELRRLGYRDAVVERESVQTD
                     PLTGKVSVAVRVEENRRWWVTQLETRHQDPLQGPVGSVQTTLLREPFSPAWEQECIRS
                     LRNAEYAKGLPDARVTIETETLRETENEVEVRVLARVKRGAPFRVGEVTFDGLNRTRE
                     SLVRKRVPLIRGEPLDRLAIDETRLQLLRLGVFDRAEAEIAPATAEDARDVRFELREA
                     KPIEASLLAGWGSYDLLWGGFELEQINLFGLAHRQRLVAAQSLKSTKLEYAYTIPQVF
                     SPLGEVTGLGFYRRREETTFTRRESGASLSYQRLVPSLHSSIGLRFTFEELSADDSHF
                     DPDYGRKRASASSVGLTLDHNRTDNALYPSRGYRLFVTTELATDTFGGTANYTRFELG
                     GSWHRPLSRTLIAHLGASHAWVTTPGLVGDDLPFNKRFFLGGASSVRGYVQGEAASYD
                     PAGRLVG"
     gene            complement(1890..5648)
                     /locus_tag="PKK20_03005"
     CDS             complement(1890..5648)
                     /locus_tag="PKK20_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNU98887.1"
                     /translation="MNRPAAKLTTSQRVVRTLRVLGAAAAATLVAVALLPFWLPPALK
                     LGLPRLGVQAGAIDRIGYATLQLQEVHFQSDTMDITLRDMRLPQPIAWAWRRWLNPAP
                     DTNAIIRLTGCVVLPKRADPSEKRGGKLPARLSGMLDAANELIPGLRKWLPPLDLNEG
                     RIVLSEHEILVPELRWRAGELKATAEIGQLGINTRISIAAHAPGRLSFRLQDLSRQVE
                     LKAELHAGESEWGLTGVGFWLDNVVHLRAKHGRNSKGLWPDRVELEAPQVKLSGRVLG
                     VPGCSNVLGQVRLFGTEGRFELRVSGRTEPASVDLPPGRLNIEALVNPTEISVTGFEA
                     RIGTIDAVLSNPVTWRWASRQIDRPARLTLQADLDAQSWIDMGGRIQGDVLLTLGEGL
                     EPRAEFEFAGKALTSRWIDVSTLELSGRTSGRLIERCAVSARWENGSELRVVGRGLDP
                     AGRTLSDAQLEAVIEPGLPFLGKWPLTFQSATARATVSGAWTNLQHRGTVTVSGGSVR
                     GLKTFDLTGRWEGDRAERIWSSVILTTDGGKAAFSGTIEDLSNLTLSALSLRALDEPP
                     LVLREPARLKILAPAAPLPGPRWSVQTSPLTLADGRRQIALEGLIEWPARAQATLAVT
                     NLNTRFLDAFLARRLPMAALESLAVEVSGGRGPAMLTVAASGWWQAAPDFPIRMNARF
                     KGDDSGLQIAEFRMFEGEAEALRIEGGIPLVCGFLETGRLWHLSSGRGLELDFSLALT
                     PTIEMLGLERLGFAAANLRCEGSVSGTPAAPVGEMRLRASNLLTLTSSPLAGWLPRID
                     DVDLILRADADHVRLDSARLEVEGAPLDVSIALPVSRDFWVAALRGAFEADPANLSGS
                     ARLDALPLARLASRFVPELRPEGTLSADVQLLAGWRWSGNLEFGGFGTRPLPFIGPLR
                     DLHGRMVLRDRRARLEGVAADVGGTTAQIEGWFDLPAVSGIEWRTPAFDIRLGGTQLA
                     LVRQADLIVRGDLDLRLQHGQTDAPSLAGTVVLGRSFVLSDLEAVLGNRLRAPERPPP
                     FFSVVQEPFAGWRLDVDIQGDECIHARSPFYTGVHSVHLKLRGTLRDPVLLGDARVVR
                     GNLTFPYATLPVRQGLVTFTETDPTRPRVQASAGARAFGYDLILDASGTLTDPVIRFS
                     SSPPLSSEDILLMITVGELPRQQMDATAADRLSKLAILLGKDLMNKLGKPGSGEGQII
                     YRAGERVSARGKVTRHLEYRLTDTWSLVAEYDEFDELSAGVKWRVLRR"
     gene            5898..7202
                     /locus_tag="PKK20_03010"
     CDS             5898..7202
                     /locus_tag="PKK20_03010"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNU98888.1"
                     /translation="MRKHKSASGRAIRRGASWRDGSGLAPAVMCALLAPTHGYGFEAK
                     DLLAFSVGPLVVRPSVGASETYNDNLFYNSGSEESAFITGVTPAVDVRLGRLDGDRIL
                     HFKYDFNHYWYHGVDMIDTAVAHNLALTGTWRGSRFLSDTAFSFGFMDTVYGGYESFE
                     QGGVVGGSNVERTTLDFSQRLRYQISQKTAAYGRFTLNNLDFDESQYSSFYDRNVWRV
                     TGGFGYQVRPKVEAIGEVHYGQTSSDPNMPFRDKPPHLETIGGFLGANLSFTTKLTGT
                     AKVGYEQVSAEPNEVGADLDFGAPIAQVSLTSALTERSSATLSYNRGTGASVQSSAAV
                     YTSDYLRLQYRHALGTAHPVVVTLGGSYGLNSYESRLTAAGRQPGYDRDILGANASIA
                     YQPRPWFTVGLYYKYTQSDGSSPWAGVDYAVNEITLALSLGY"
     gene            7205..7795
                     /locus_tag="PKK20_03015"
     CDS             7205..7795
                     /locus_tag="PKK20_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNU98889.1"
                     /translation="MRHIRLHGAALAGLALWIGTAICARPIAQVAGTEPERRVAANDL
                     LFITIVGERDLQTEFRVSSAGTIQFPFLDEVSVGGLTPAELRARLRDLLMKDYFVDPQ
                     VIVTVKDYRSEFVRVVGQVNRPGPIPHTGEQKLDVYDIITMAGGVTPRARNSVEYTRN
                     GVTRTLKLDDLKKETDPAKKIWVQPGDIIEVKESVL"
     gene            7805..9979
                     /locus_tag="PKK20_03020"
     CDS             7805..9979
                     /locus_tag="PKK20_03020"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNU98890.1"
                     /translation="MSYSYYTSQAAPPEASESSSSNLNLRHYWNIVLQRRWLVIATFV
                     SVFVLCLVYLFKAPRIYSATARLQIDRESAASLNLNEVVMSMTDQDYLQTQYRNLLSR
                     SLLEIVIKREKINEDSRYRDSPDVARALAEDIRINPIRLSRLVDVVVEHPNASKAAGI
                     ANALVEEFIEQNLVQKQKRTMDMLFFLRSQANGLEVDVAKAEEDIQNYRAQVKFVSLD
                     SSYNIVAEALSQSQAQYAMAKAQAQTTQTIVEELDRHIQAGNPLETFPKIAADGKVNS
                     LQLQLSGQENELAGLLKRYKDKHPAVIQARSKIEETHRSVQGAAQQVAEILRSEARLA
                     KASEQILAANVKEWEDQQMEWNKAKMQYDVLMRKAQSSKALFDTVLAKMKEIELVQRD
                     KGNNIRVIDYAYAATMPVKPRVLLTLFLGAFGGLGLAIGLAFFVNFLDDSIKSQDDVE
                     LYLHLPFLGYIPNIKTNSVVESGLQAHLHPQSNAAESFRTVRAAIALGAKAEKMRVFT
                     VTSTIPSEGKSLVASNLAVVVAQTGLRTVLVDADLRRPSLHKAFQLHSPMGLTAYLTE
                     QTNRVDDVIQTTEVTNLDVACCGQIRSQPSELLGSQRMVQFMQELSKRYDRVILDCPP
                     VSAVSDPLILSAMADAVVFVTKFNKVRRDHARRTIQRIQDAGIHICGVVLNDIDFEGR
                     DSYYYSYTYYQNRYYSSYHRERAESRRQAVVESSGSTPAPHA"
     gene            10298..11245
                     /locus_tag="PKK20_03025"
     CDS             10298..11245
                     /locus_tag="PKK20_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNU98891.1"
                     /translation="MGIAREVVGIEIGGTKLQIVVGTDRGRILDRFRFDVEPGQGAAA
                     IRRQIEITVEHLHSKKAQSAIAVGFGGPVDWRTGRVCCSHHVEGWSGFPFAAWLRERF
                     GLPVVIDNDANVAALAESRLGAGKGANPVFYVTLGSGVGGGLVVDGRIYHGATPGEAE
                     IGHVRLDRSGATVESRCAGWAVDRKVRAVKMAQPHGLLARLVGQETYGEARHLKAAIE
                     HGDATANTILRETASDLAFALSHVIHLFHPEVIVLGGGLSLIGEPLRKAVLAALPPHV
                     MAAFQPGPDVRLAQLREDAVPIGALVLASSISELPPAKP"
     gene            11277..12059
                     /locus_tag="PKK20_03030"
     CDS             11277..12059
                     /locus_tag="PKK20_03030"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HNU98892.1"
                     /translation="MNDTSATQPFQSEAIPFDSAPRSNPENLWLNLLFGAVIPAILLA
                     YGSKEKYLGPAWGMIVALSFPLGYAIYDLVRRRTWNVISIVGFVGTLLTGSLGLLKMS
                     GFWFAVKEAAVPLVLGLAIPASLRTRQPLVRTLLYNDQLLDTKRIHRALEQRRNIAAF
                     DQLLRWASWILAGAFLISAVINFALAWWLLPAQSGTEEFNRQLGKLQFWSWPGVVIPS
                     SAMVFYAMFRLVKGLEHMTGLKGDELFHPKPAKPKRADKARS"
     gene            complement(12156..>13220)
                     /locus_tag="PKK20_03035"
     CDS             complement(12156..>13220)
                     /locus_tag="PKK20_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98893.1"
                     /translation="KRHGLKRSQVLLNSSHTHAGPVIWPNLRVLFDFDEAEQARVVDY
                     TRRLCDNLTAVIDAALDDLAPAHIASGHGAAHFAINRRQPTSNGVRIGQNRSGPVDHD
                     VPVLSITGDGGALRAVVFGYACHNTTLGGNFYHVDGDYAGCAQRELEKAHPGATALFL
                     MLCGGDQNPNPRGTYEHVEQYGRSLADAVERVLSCGLKPVRPPVRSAWRQTQLDFAPH
                     TRATFEEEAKSADRFRQRRARLMLDTYDGKRPIRKLTYPVQAIRLGNDWTLLGLGGEV
                     VVDYAQRAKTEYPKDNLTVAGYCNDVSCYIVSDRVLSEGGYEPVDSMIYYAQPGPLAP
                     MSEERVFAAIRRVMRKAGAR"
BASE COUNT         2553 a         4223 c         4072 g         2372 t
ORIGIN      
        1 acccacaagc ctcccggccg ggtcgtagga tgcagcttcc ccctggacgt acccccggac
       61 cgaactggcg ccgccgagga agaaccgttt gttgaacggc aagtcgtccc ccaccaaacc
      121 gggcgtggtc acccaggcgt gggatgcgcc gagatgcgcg atcagagtcc gcgacagcgg
      181 tcggtgccag cttccgccca actcgaagcg ggtgtaattg gccgtgcctc cgaacgtgtc
      241 ggtcgccagt tccgttgtga cgaaaagccg ataccctcga gacggataaa gggcattgtc
      301 cgtgcgatta tgatcgagcg tcaggccgac tgacgatgcg ctggcacgtt tccgcccata
      361 atcgggatcg aaatggctgt cgtcggccga gagttcctcg aatgtgaaac ggaggccgat
      421 ggaactgtgc aacgagggaa ccagccgctg atagctcagg cttgccccgg attcacgacg
      481 ggtgaatgtg gtctcctccc gccgccggta aaaaccgagc cccgtcactt ctcccagggg
      541 actgaacact tgggggatgg tgtaggcgta ttcgagcttg gtgctcttga gcgattgcgc
      601 ggccaccaga cgctgcctgt gggcgaggcc gaacaggttg atctgctcga gctcgaaacc
      661 gccccagagc aggtcgtaac tcccccaacc agccagcagg ctggcctcga tcggcttggc
      721 ctcgcgcaac tcgaagcgga cgtcacgcgc gtcctcagcc gttgcagggg cgatctcggc
      781 ctctgcccgg tcaaagaccc ccagccgcag caactgcagg cgcgtctcgt cgatggccag
      841 ccgatcgagc ggctcgcccc gaatcagcgg cacccgcttg cgaaccagag attcccgagt
      901 gcggttcagg ccgtcgaacg tgacctcgcc gacccggaac ggggcccctc gtttcacacg
      961 ggccagcacc cggacctcga cttcgttctc ggtttcccgg agcgtctccg tctcgatcgt
     1021 cacccgcgcg tcagggagtc ccttcgcgta ctcggcgttg cgaagcgatc ggatgcactc
     1081 ctgctcccac gccggcgaaa agggctcgcg cagcaaggtg gtttggacgg aaccaacggg
     1141 gccctggagc gggtcttggt gacgcgtctc caactgcgtc acccaccagc gccggttttc
     1201 ctcgacacgc acggccacgc tcaccttgcc ggtgaggggg tctgtttgga cagactcccg
     1261 ttcgaccacc gcgtcccgat agcccaatcg gcgcagttcc tcgcggagac ttgccagccc
     1321 gcgatcgagc cgggcgggtg tgtacacctt cggctttttc tgaggcacca ccatgtcggg
     1381 caccaggaag cagccggccg ctttcgcttt ggacagggca gtcaagccgg tgaaatccag
     1441 tcgtgcgtag taatgaaacg cgccgcgatc gagcaggaac accgcgcgtc gtgcgcgcca
     1501 atgcgccggt gcctcgaggg gagcctccgg cctggcggac aacaccgatt cagtcccgtc
     1561 gtcgcagagc acctgcaact cgatggcggg ttcgaggtag ccctcgcgct gcagggcgga
     1621 aagcaggagc agggctgcat cctcgagaat gcgcgaatcc aggaattccg ggggtcgcga
     1681 gtccggctgc agcacccgca actgctgttt gaggcgcaga ttccccaaga aaccatggcc
     1741 tcgcacaatc aatcgagccg gtttgatctt gggctcggtt agcgaattcg gtgcggtcgg
     1801 tggactggcc ccagtccggt ttggactgcc gaggagaagg gccgcagcga gacgagccgt
     1861 ccaaatccac gccttgcctc ggggacggtt catcggcgca agactctcca cttgaccccg
     1921 gcgctcagct cgtcgaactc atcatactcg gccaccagcg accacgtgtc ggtcagcctg
     1981 tattcgagat ggcgagtcac cttgccgcgc gcggagacac gctcgccggc gcgatagatg
     2041 atctgcccct cgccggaccc cggttttcca agcttgttca tcagatcctt gcccaggagg
     2101 atggcgagct tcgagaggcg gtcagcagcc gtggcgtcca tttgctggcg gggaagttcg
     2161 cccacagtga tcatcaacag gatgtcctcg gaactcagcg gcgggctcga tgaaaaccgg
     2221 atcacagggt ctgtcagagt gccgctcgca tcgagaatga gatcgtatcc aaacgcccgc
     2281 gcgccggccg aggcctgcac gcgaggccgc gtgggatcgg tctcggtgaa agtcaccagc
     2341 ccctggcgca ccggcagtgt cgcatacgga aaggtgagat tgccgcgcac cacgcgcgcg
     2401 tcgccaagca acaccgggtc gcgcagtgtg ccgcgcagct tgagatgcac cgagtgcacg
     2461 ccggtataga acgggctgcg cgcatggatg cattcgtcgc cctggatgtc cacatcgagc
     2521 ctccagccgg cgaatggctc ctgcacgacg ctgaagaacg ggggcgggcg ctcgggggcc
     2581 cgcaggcggt tgcccagcac ggcctcgaga tcgctgagca caaagctgcg ccccaacaca
     2641 accgtccccg ccagactcgg cgcatcggtc tgcccgtgct gcaaacgcag atcgaggtcg
     2701 ccgcgcacga tcaggtcggc ctgtcgaacg agcgcgagtt gcgtcccgcc caggcggatg
     2761 tcgaacgccg gcgttctcca ttcgatcccg gagacagcgg gcaagtcgaa ccagccttcg
     2821 atctgagcgg tggtgccccc gacgtcggcg gccaccccct ccaaccgcgc ccgccgatca
     2881 cgcagcacca tgcggccgtg caggtctcgc agcggtccga tgaacggcaa gggacgcgtc
     2941 ccgaagccgc caaactcgag attgccgctc cagcgccagc ccgccaggag ttgcacatcg
     3001 gcgctgaggg tgccttcggg ccgcagttcg gggacgaatc tcgacgccaa tcgagccagc
     3061 ggcagcgcat cgagacgcgc ggagccgctc aggttcgcgg ggtcagcctc gaacgcccct
     3121 cgcagggccg cgacccagaa gtccctcgac accggcagcg cgatcgagac atcgaggggc
     3181 gcgccttcca cctcgagtcg ggccgagtcc aggcgcacat ggtccgcatc cgcccgcaga
     3241 atcaggtcca cgtcgtcgat ccgaggcagc cagcccgcca acggggaact ggtcagagtc
     3301 agcaggttgg acgcgcgcag acgcatttca ccgacaggcg cagccggcgt gccggacacc
     3361 gaaccctcgc aacgcaggtt ggcggcggca aagcccaacc gctccaatcc aagcatctcg
     3421 atcgtcggtg tcagtgccag cgagaaatcc aattcaagcc cccgacccga cgaaaggtgc
     3481 cagagccgcc ccgtctcgag gaaaccgcac acgaggggaa tgccgccctc gatgcgcagg
     3541 gcctcagcct cgccctcgaa cattcgaaac tcggcgattt gcaggccgct gtcatcgccc
     3601 ttgaacctgg cgttcatccg gatcgggaaa tccggagccg cctgccacca accgctcgcc
     3661 gccacggtga gcatcgccgg accgcgcccg ccgctgacct cgaccgccag cgactcgagg
     3721 gcggccatcg ggagtcgacg cgcaagaaaa gcgtcgagaa accgagtgtt aagatttgtc
     3781 actgcaagag ttgcttgggc gcgcgcgggc cattcaatga gcccttcgag agcgatctgc
     3841 cgccggccat ctgcgagtgt cagcggggag gtctggacac tccatcgcgg accgggcagc
     3901 ggcgctgctg gagcgagaat cttcaggcgt gccggttcac gcaaaaccag cggcggctcg
     3961 tcgagcgcgc gcaggctcaa ggcgctgagc gtgaggttcg acaggtcctc gattgttccg
     4021 ctgaacgccg ccttgccacc atcggttgtc agaatgactg aagaccaaat cctctccgcg
     4081 cgatcccctt cccaacgccc ggtcagatcg aacgtcttaa ggccgcggac ggatccgccg
     4141 gacaccgtca ccgtcccgcg atgctgcaga ttggtccatg caccggagac ggtcgcgcgg
     4201 gcggtggcag attggaatgt cagaggccat ttccccagga acggcagccc aggttcgatg
     4261 acggcctcga gttgggcatc ggacagtgtt cggccggcgg ggtccaatcc gcgcccaacc
     4321 acgcggagtt cggagccgtt ttcccaacgc gcgctaacgg cgcaccgttc gatcaaccgc
     4381 cccgaggttc gtccactcaa ctccaaagtc gaaacatcga tccagcggct ggtcagagcc
     4441 ttcccggcga actcgaattc cgcgcgcggt tcgagtcctt cgcccagtgt cagcagcacg
     4501 tcgccctgaa tccggcctcc catgtcgatc caggactgcg catccaggtc cgcctgcagc
     4561 gtgagtcgcg ccgggcggtc gatctgccgg cttgcccaac gccaagtcac gggattcgaa
     4621 agcacggcat cgatggtccc gatccgggct tcgaagccgg tgacgctgat ttccgttgga
     4681 ttgaccagag cctcgatatt cagccgaccg ggtggcaggt ccacgctggc cggttccgtg
     4741 cgaccgctga cgcgcaactc gaatcggcct tctgtcccga acaagcggac ctgtccaagc
     4801 acattcgagc accccggcac cccaagaacc cgccccgaca gcttgacttg cggcgcttcg
     4861 agttcgacgc ggtccggcca caaccccttg ctattgcgcc catgcttcgc ccggaggtgg
     4921 acaacgttgt cgagccagaa ccccacgcct gtcagacccc attccgattc cccggcgtgc
     4981 aattcggcct tcaattcaac ctgccgcgac aggtcctgaa gccgaaatga gagccggccc
     5041 ggtgcatggg ctgcgatgct gatccgcgtg tttataccaa gttggccgat ttccgccgtg
     5101 gccttcaact cgcccgcccg ccaacgcagt tcgggcacga gaatctcatg ctccgaaagc
     5161 acgatgcggc cttcattcaa gtcgaggggg ggcagccact tcctgagtcc gggaatcagt
     5221 tcgttcgccg catcgagcat cccggacaat cgcgccggca gcttgccccc cctcttctcg
     5281 gagggatcag ccctcttggg caagacgacg cagcctgtga ggcggatgat cgcattcgta
     5341 tcgggagcgg ggttgagcca gcgtcgccaa gcccaggcaa tgggctgcgg caggcgcatg
     5401 tcgcgaagcg tgatgtccat tgtatccgat tggaaatgaa cttcctgcaa ctgaagggtc
     5461 gcatatccaa tccgatcgat cgcgccggcc tgaaccccaa gccgcgggag tcccaacttg
     5521 agcgccggag gaagccagaa cggcagcagc gccacagcga ccaacgtggc cgccgcagcc
     5581 gcgcccaaca cgcgcaacgt tcgaacaacg cgttgcgatg tcgtgagttt ggcggcggga
     5641 cgattcacag acacaccgca ttaaggagac gcggcgggcg ccgtgtcaaa gcgatatgga
     5701 aatcgcgctt gccacagtgt gtccgttcgt ggattctgca cgaaacgcaa ccggaaacgc
     5761 gcggccatgt cgggatcggc agtcatgcga tcgcgagggg cgggcatcgg ggcttgcgag
     5821 ttgacttcgc agcctgcgtc gcgcctagga ttcgcaccac accgcatagt cgtgcgaggg
     5881 gtaacaggac cacaatcatg agaaaacaca aatcggcttc aggaagggcg atacgccgtg
     5941 gcgcttcatg gcgtgatggg agcggtttgg cgcccgcggt gatgtgcgct ttgttggctc
     6001 cgacgcacgg ttacggcttc gaagccaagg atttgctggc cttcagcgtt ggacctttgg
     6061 tggtgcgacc gagtgtgggt gcgagcgaga cttacaatga caacctgttc tacaactccg
     6121 ggtctgagga gtctgcgttc atcacgggcg ttacgcctgc tgtcgacgtg cggctgggcc
     6181 ggctggatgg cgaccgaatt ctccatttca aatacgactt caaccattac tggtaccacg
     6241 gagtcgacat gatcgacacg gctgtcgccc ataacttggc gctgacagga acctggcgcg
     6301 ggagccgatt cctctcggac acggcgtttt ccttcgggtt catggacacc gtctatggcg
     6361 gttacgagag tttcgagcaa gggggcgtgg tgggtggatc caatgtcgag cgcacaacgt
     6421 tggacttcag tcaacgattg cgctaccaga tcagccagaa gaccgccgcc tacgggcgct
     6481 tcacactgaa caacctcgat ttcgatgagt cccaatacag ctctttctac gatcggaatg
     6541 tgtggcgggt cacgggcgga ttcgggtacc aggtccgccc caaagtcgag gcgatcggcg
     6601 aagtccacta cggccagacg tccagcgatc caaatatgcc cttccgggat aaaccaccgc
     6661 atctcgagac gatcggcggt ttcctggggg cgaacctgag cttcaccacg aagttgaccg
     6721 gcacagccaa ggtgggttac gagcaggtga gtgcggagcc gaacgaggtg ggagcggacc
     6781 tcgatttcgg agcgcccatc gcgcaggtct ccctgacctc ggctctgacc gagcgcagca
     6841 gcgccacgtt gtcctataat cgggggactg gagcatccgt gcaaagcagc gcggccgttt
     6901 acacaagcga ttatctcaga ctccaatatc gccacgcttt gggaaccgcc cacccggtgg
     6961 ttgtcacgct cggcgggagt tacggattaa actcgtacga atcgcgcctc acggccgcgg
     7021 gccgtcagcc cggatatgat cgggacattc tcggagcgaa tgccagcatc gcttatcaac
     7081 ctcgaccgtg gttcacggtt ggcttgtact acaagtacac gcaatcggac ggatcgtcgc
     7141 catgggcggg tgttgactac gcggtgaatg agattacttt ggccctatca ttggggtatt
     7201 agcgatgaga cacattcgat tgcatggggc ggctttggcg ggtttggcgc tttggattgg
     7261 aacggcgatc tgcgctagac cgatcgccca ggtcgccggc acggaacccg aacgcagagt
     7321 cgccgcgaac gatctgctgt tcatcaccat cgtgggcgaa cgcgacctgc agaccgagtt
     7381 ccgcgtctcc tccgcgggca cgattcagtt cccgttcctc gatgaggtca gcgtcggcgg
     7441 tctgactccg gcggaactgc gcgcgcgctt gcgcgacctg ctgatgaagg actatttcgt
     7501 cgatccgcag gtgatcgtca cggtcaagga ttaccgctcc gagtttgtgc gggtggtagg
     7561 acaggtgaat cgaccggggc ccatcccgca cacgggcgaa cagaaactcg atgtttacga
     7621 catcatcacc atggcgggcg gagtcactcc ccgcgcccgg aactcggtcg agtacactcg
     7681 taacggcgtg acccggacgc tcaagctgga cgacctcaag aaggaaaccg atccggccaa
     7741 gaagatttgg gtgcaacccg gcgacatcat cgaagtcaag gagagcgtcc tctaatcgcg
     7801 cctcatgtcc tattcctatt atacctctca ggcagccccc cccgaggcct ccgaatcgtc
     7861 atcgagcaac ctgaatctgc ggcactattg gaatatcgtg ctgcagcgtc gatggctcgt
     7921 gattgcgacc ttcgtctcgg tgtttgttct gtgtctggtc tacctgttta aggcgccgcg
     7981 gatctactcg gcgaccgccc gtcttcagat cgaccgggaa tccgcggcga gcctcaacct
     8041 gaacgaggtc gtcatgtcga tgaccgatca ggactatctc cagacgcagt atcgtaatct
     8101 cctcagccgc agcctgctcg agatcgtcat caagcgcgag aaaatcaacg aggactcccg
     8161 ctatcgcgac agcccagacg ttgcccgcgc tttggcggag gacatccgca tcaatccaat
     8221 ccgtctgagc cgcctggtgg atgtcgtggt cgagcatccc aatgcgtcca aggcggcggg
     8281 catcgccaac gcgctcgtgg aggagttcat cgagcagaac cttgttcaga agcagaagcg
     8341 gaccatggac atgctcttct tcctgagaag ccaggccaat ggtctcgagg tggacgtggc
     8401 caaggcggag gaggacattc agaattaccg ggctcaggtg aagttcgtgt cgctggacag
     8461 cagctacaac atcgtggcgg aggcgctcag ccagtcccag gcgcaatacg caatggccaa
     8521 ggcgcaggcc caaaccactc aaaccatcgt cgaggaactg gatcgtcaca tccaggccgg
     8581 gaatcccctc gagaccttcc cgaagatcgc tgccgacgga aaagtgaact cgctgcaact
     8641 ccagctctcc ggccaggaaa acgagttggc tggtcttctc aaacgctaca aggacaagca
     8701 ccccgccgtg attcaagcgc ggtcgaagat cgaagagacg caccgctcgg tgcagggcgc
     8761 ggcccaacag gttgcggaaa tcctccgctc cgaagcgcgc ctggcgaagg cttcggaaca
     8821 gatacttgct gccaacgtca aggaatggga agaccagcag atggagtgga acaaggcgaa
     8881 gatgcaatac gatgtcctga tgcgcaaggc ccagagcagc aaagcgttgt tcgacacggt
     8941 cctcgccaag atgaaggaaa tcgagctggt ccagagagac aagggcaaca acatccgggt
     9001 tatcgactac gcatacgccg cgaccatgcc ggtcaagcct cgagttctgc tgacgttgtt
     9061 cctgggggct tttggaggat taggactcgc gatcggcctg gcgtttttcg tcaacttcct
     9121 ggacgactcg atcaagagcc aggatgacgt cgagttgtac ctccatctgc cattcctcgg
     9181 gtacattccc aatatcaaga ccaacagcgt cgtcgagagc ggcttgcagg cccacctgca
     9241 tccgcagtca aatgccgccg aaagcttccg caccgtccga gccgccatcg ctctgggggc
     9301 caaagccgag aagatgcggg tgttcactgt gacgagcacc atcccctccg agggcaagtc
     9361 cctcgtcgca tcgaacctgg ctgtggtcgt tgcccagacc ggtttgcgca cggtgcttgt
     9421 cgacgccgac ctgcgccgac cttcactcca caaggccttc cagcttcaca gtcccatggg
     9481 cctgacggct tacctcaccg aacagaccaa ccgggtcgat gatgtgatcc agaccaccga
     9541 ggtgaccaat ctggatgtag cctgctgcgg acagattcgt tctcagccat cggaattgct
     9601 cggatcccaa aggatggttc agttcatgca ggaactgagc aagcgctacg accgggtgat
     9661 cctcgattgc ccgcctgttt cagccgtgag cgatcccctg atcctctcgg ccatggccga
     9721 cgcggtcgta ttcgtcacca agttcaacaa ggtccggcgc gatcacgcgc gccgaactat
     9781 tcagcgcatc caggacgccg gcattcacat ctgtggtgtc gttttgaatg acattgattt
     9841 cgagggacgc gattcctact actactccta cacgtactat cagaatcgtt actactcgag
     9901 ttaccaccgg gagcgggctg aatcgcggcg gcaggccgtc gtggaaagtt caggatcgac
     9961 ccctgcccca cacgcttgat gccgcttggc ccgcatgttt cgtcgagcct tgaccgcccg
    10021 cgaagaccag tgcatctgga gggtgtcggt gggaatcgag tatgcgccgc gtccgggcac
    10081 gcggcctaca tcggcaatct ccatccgttg taggcccggt gccctcaccg ggcgacagaa
    10141 caccgacaac aaccagatgc accgcgcgga gaccaaggcc tcgagaggcg cgtgcctcgc
    10201 cggccaacag gattctcatc accccctgtc gccccgcggg cgcaagatcg ctgagagccc
    10261 ctgaagcggg cagagctatc gactccggga cttggccatg ggcatcgcgc gtgaagtggt
    10321 tggaatcgag atcggcggaa ccaaactgca aatcgttgtc gggactgacc gcggacgcat
    10381 tctcgatcgc tttcggttcg atgtggaacc agggcaaggg gcggcggcca tccgccgtca
    10441 gatcgagatc acggtcgaac acctccattc caagaaggcg cagtccgcta ttgctgtcgg
    10501 ctttggtggc ccggtggact ggcgcaccgg gcgtgtttgt tgttcccatc atgtcgaggg
    10561 ttggtcgggt tttcctttcg ccgcatggct ccgcgaacgg ttcgggctgc cggtcgtgat
    10621 cgacaacgac gcgaatgtgg ccgcattggc ggagtcgcgc ctgggggctg ggaaaggagc
    10681 gaatccggtc ttttacgtca cccttgggag cggcgtgggc ggaggtttgg tggtggacgg
    10741 ccggatttac catggcgcga cgccgggcga agccgagatc ggccacgtgc gcctcgaccg
    10801 atccggtgca accgtcgagt cgcggtgtgc gggttgggcc gtcgatcgca aagtgcgggc
    10861 tgtcaagatg gcccaaccgc acggattgct cgcccgtctg gtgggccagg agacatacgg
    10921 cgaggcaagg catctcaagg cggccatcga acacggagac gcgacggcga acaccatcct
    10981 tcgggagaca gcctccgatc tcgcgtttgc cctctcccat gtcatccatc tgtttcatcc
    11041 ggaggtcatc gtgcttggag gcggcctgtc gctgatcgga gagccgttgc gaaaggcggt
    11101 gctcgccgcc ctgccgccgc acgtcatggc cgctttccaa cccggacccg acgtgcgttt
    11161 ggcacagttg cgtgaagacg cggtgccgat cggagcgctg gtcctggcaa gctcgatatc
    11221 cgaattgcct ccagcgaagc catgagacag ttcgacgcgg atagaagcgc cagacaatga
    11281 acgatacatc ggccacgcaa cctttccaaa gcgaggcgat cccattcgat tcggccccgc
    11341 gctcgaatcc agagaatctc tggctcaacc tgctgttcgg cgcggtcatt ccggcaatcc
    11401 tgttggccta cggcagcaag gagaaatacc ttggcccggc atggggcatg atcgtggccc
    11461 tgtcgtttcc gctcggctat gccatctacg atctggtccg acgacgcact tggaacgtca
    11521 tctcgattgt cggattcgtg ggcacgctgc tcacgggcag cctcggtctg ctcaagatga
    11581 gcggtttctg gtttgccgtg aaggaagccg ccgtgccttt ggttctggga ctggcaattc
    11641 cggcctccct gcgcacgcgc caaccactgg tccgcacctt gctgtacaat gatcaactgc
    11701 tggacaccaa gcgaatccac cgcgctctcg aacaacggcg caacatcgcc gccttcgatc
    11761 agttgctgag atgggcctcg tggattctgg ctggcgcgtt cctgatcagc gccgtcatca
    11821 atttcgcgct ggcctggtgg cttctcccgg cgcaatcggg caccgaggag ttcaaccgcc
    11881 aattggggaa actccagttc tggagctggc cgggcgttgt gatcccaagt tcggcaatgg
    11941 tcttctacgc catgttccgc ctcgtcaaag gtctcgaaca catgacggga ttgaagggag
    12001 acgaactgtt ccatccgaag ccagccaaac cgaagcgagc cgacaaggcc cggtcgtgat
    12061 ctgacacctg acgccctcgc acgcgggttg ggggcgtcgc gctcccactg cggatgcaat
    12121 cggccactct tccaatccga tcccccctcg gcccgtcaac gcgcgccggc cttgcgcatc
    12181 acgcgtcgga ttgcggcgaa cactcgctcc tccgacatcg gcgcaagcgg ccccggctgg
    12241 gcatagtaga tcatgctgtc gaccggttca tatccgccct cgctcagcac cctgtccgat
    12301 acaatgtaac aactgacatc gttgcagtac ccagccacgg tcaggttgtc cttcgggtat
    12361 tcggtcttgg cgcgctgcgc gtagtccacg acgacctcgc cgcccaaccc caaaagagtc
    12421 cagtcgtttc ccagccggat ggcctggacc ggataggtca gtttacggat tggccgcttg
    12481 ccatcgtatg tatcaagcat cagccgcgcg cgccgctgac ggaatctgtc ggcgctcttc
    12541 gcttcttcct cgaaggtcgc gcgtgtatgc ggggcaaaat cgagctgggt ctgccgccac
    12601 gcgctgcgga cgggcgggcg cacgggcttg agcccgcagg agagaacgcg ctcgacggcg
    12661 tcggccagag accggccgta ttgctcgaca tgctcgtacg ttccgcgggg attgggattc
    12721 tggtcaccgc cgcagagcat gagaaacagc gcggtggcgc ccggatgcgc cttctcgagt
    12781 tcgcgctggg cgcacccggc gtaatcgccg tccacgtgat agaaattccc acccagcgtg
    12841 gtgttatgac acgcatagcc gaatacaacc gcccgcagcg ccccgccgtc cccggtgatg
    12901 ctcaacacgg gcacatcgtg atccacgggc ccagaccggt tttgcccgat ccgaacgccg
    12961 ttggacgtgg gctggcgtcg gttgatggcg aagtgggcgg caccgtgccc ggaggcgata
    13021 tgggcagggg cgagatcgtc gagcgcggca tcgatcaccg ctgtcagatt gtcgcaaagc
    13081 ctgcgcgtgt aatcgacgac gcgcgcctgc tcggcctcgt cgaaatcaaa caagacgcgc
    13141 aggttgggcc agatcaccgg cccggcatgc gtgtgcgacg agttgagcaa gacctgcgat
    13201 cgcttcagcc cgtgacgttt
//
LOCUS       DAOQRO010000047        13124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3813_length_13124_cov_5.549468, whole genome shotgun sequence.
ACCESSION   DAOQRO010000047 DAOQRO010000000
VERSION     DAOQRO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_13124_cov_5.549468"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKK20_03040"
     CDS             <1..382
                     /locus_tag="PKK20_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNU98894.1"
                     /translation="ICRFVNPRTVVLIREPNRRDINYRPLAENWERIRDVRLEDGSRP
                     EVVPLPMPAPLFFEKDRLPASYANFYIANAAVLVPTFNDPNDRIALGILAELFADRPV
                     VGIHAVDLVLGFGSIHCLTQQQPR"
     gene            complement(553..1575)
                     /locus_tag="PKK20_03045"
     CDS             complement(553..1575)
                     /locus_tag="PKK20_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNU98895.1"
                     /translation="MARKLRIEYPGAVYHVMSRGNQGGLIFRGDRDRKLFLETVAEGC
                     EKTGWRIHAYVLMPNHYHLLVETPEGNLVAGMRWLQGTYTQRYNARNDVFGHLFQGRY
                     KALIVDGGADRYFQVVSTYVHLNPARAGLIRIGEQALSRYRWSSYPDYLRGRAGRPPW
                     LEVKRVLGSVGIGADDARGRQEYEAHVEGRVLELGRRRSREALEEEWRRVRRGWYLGG
                     PAFRQRLVKWLEKAVAGKARKSHAGEARRAHDEAAAEVLLAQGLALMGLKKGDLAALA
                     KGAKEKQVLAWWLRKRTVVSRAWVSRRLQMGDESRVTQAVAAVRRASDRQMAGWRRRL
                     EAETDP"
     gene            1708..2385
                     /locus_tag="PKK20_03050"
     CDS             1708..2385
                     /locus_tag="PKK20_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNU98896.1"
                     /translation="MTTEPIKLEDAVEFAENPEPRCPCVLLLDTSGSMRGEPIAALSE
                     GLKAFKDDLTKDALASRRVELAVVTFDSDVKVVQDFVTVDQFEPPELAAQGQTFMGSA
                     IHKALDMIQARKAKYRANAVAYYRPWVFMITDGEPQGEPDSIIQQASQRLKSDEANKR
                     VAFFAVGVENANMSRLAELVVRAPVKLIGLNFVEMFVWLSRSMEKVAQSKTDEQVALP
                     PPGWGTV"
     gene            2400..3182
                     /locus_tag="PKK20_03055"
     CDS             2400..3182
                     /locus_tag="PKK20_03055"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNU98897.1"
                     /translation="MQSEAWRVAAASVRGTSHIKSDLPCQDALRWQTARGGWLLAALA
                     DGAGSASLAEIGAGIAADRGIEALKAQVECKSLADADEEWRAGVVAALASARESVLAA
                     AQERKVEARELASTLTLLAVAPGIAVAAQIGDGAALASAPPEALACLTRPMVAEYLNE
                     TTFLTSEGALESAQTVVRRGAWRHAGLLCDGLQLLALRLPEGTPHARFFEPLFGFVDR
                     ATDLTAASRELSAFLQSQRITERTDDDLSLLLAAYGNSPGSG"
     gene            3232..4710
                     /locus_tag="PKK20_03060"
     CDS             3232..4710
                     /locus_tag="PKK20_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNU98898.1"
                     /translation="MRFQRASNGDIVTLAENHRLGAGGEARIYAVPGEPRLVAKIWHK
                     PTPERSRKLRVMLANPPMDPMAAHHHASIAWPIDVLSAPGRPQALAGFIMPRVENMRP
                     VGEFSNPKVRRTRCPLFNYAYLYRTARNLATAVRALHERGYVIGDLNESNVLVAETAL
                     VTIVDTDSFQVWDAEAGVMYRCRVGKPEYTPPELQGKSFAQVDREAAHDRFGLGVLIF
                     QLLMEGTHPFAGVYRGRGEPPLLEQRIAASHFPYAREAHLFYAPKPTAPPFDTLAPGL
                     RDLCLRCFQDGHFRPSLRPEPQSWQYVIEEAESHLVTCQWNSQHVFGDHLEQCPWCER
                     AEQLAGRDPFPSIEGVKRGEHRAPPPWARERGGARSGQRRSPFWPTGMPNIPKPPPIP
                     PSAPRARTSRRRRPRPLLGDWNDWAWVALASSGLAAGAAASFQWQANPLTFLCGIFAL
                     LTGILGEAKARSPELDGRGKWIARTGLALGTGALVWCLTATV"
     gene            complement(4722..5198)
                     /locus_tag="PKK20_03065"
     CDS             complement(4722..5198)
                     /locus_tag="PKK20_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNU98899.1"
                     /translation="MARLGIISDTHNFLDPQVKSLFAGVDHILHGGDIGLPRIIMELE
                     AIAPVTAVIGNADSGLSLRETEVIELFGRRFLVHHVVEPRRLADSLRRQIDRVQPDVV
                     VFGHTHKPFRERIGETLFLNPGSAGRRRFDAPRSVAILRVGATDLEVDFHDLEPPG"
     gene            complement(5198..5803)
                     /locus_tag="PKK20_03070"
     CDS             complement(5198..5803)
                     /locus_tag="PKK20_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98900.1"
                     /translation="MKAKHGLPLVLLAVLALTRIPGLLPPNFSPVYGLLFCAAVYFSG
                     WVAWVLPLGTVLVTDVLLNVHYGVAPLQWEMLGNYAAYAGIVWLGRQFNRRSSWLTLF
                     GGGILGAILFYIVSNTVSWLHDPAYAKNLAGWIQSLTVGTPGWPHTWEFFRNTLLSGG
                     LFAGLFAGAMKLGEAVEPKPEEEKEPSEEPEEAPEPGEAKA"
     gene            complement(5936..7393)
                     /locus_tag="PKK20_03075"
     CDS             complement(5936..7393)
                     /locus_tag="PKK20_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98901.1"
                     /translation="MNSVCCLPRSNRPIPPEPSCSAPNRRQFLHLSAAAVTGLTLAPR
                     HVLGGAGQIAPSDKITLAGIGTGGQGMQNLAALQKFPEIQVVAVCDVERESNRYLSWN
                     WSQGREERTCGREPARRAVESGYAAQRGSGQYRGCAAYVDYRELLEKEDVDAVTIATP
                     DHTHAVIAMAALKRRKHVYCEKPLTYSVREARQVAEAARQAGVVTQMGNHGQAEEGAR
                     VVQEIIADGAIGAVREVHVWSGARFWTWPAWDGRPPETPPVPEGLDWDLWLGPAPDRP
                     YHPAYHPWTWRNWIDFGTGLLGDLGAHKLSTVFKALKLGHPASVEASATKLSPETYPL
                     GEIVRFEFPARGAMPPVVLTWSDGGLKPPRPPELDPNDGIEDTIYIGDQGKLMGQRLL
                     PDSRMDTYRMPPKTLPRSPGHYHEWVAGIRGGPPPGSNFVDHAGLVTEVCLLGNVAAR
                     VQQKKLLYDGPNLRILNDASANELLGRAYREGWAL"
     gene            complement(7442..9088)
                     /locus_tag="PKK20_03080"
     CDS             complement(7442..9088)
                     /locus_tag="PKK20_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNU98902.1"
                     /translation="MNSPRTSAGGLGWLALCTVWILRIEAAPPPKQTLEVPMRDGVLL
                     ATDVFLPEGEGPFPAILIRTPYNKENVGNIGAEGARRGYAIVVQDTRGRFGSEGPNLP
                     FATDGWDGAADGFDTAEWLSGQRWCNGKIGTYGGSAVGITQLLLAGAGSSRITSQHIT
                     VGAPNLYRDCVYPGGVFKRAMVEDWLRISKFSPDALTLWTSHPRLDTYWRARQLDRRY
                     GKANAAGVHIGGWFDIFAQGSIDAFVGYQERGGPRAKGRQRLVMGPWTHIVFQDKAGD
                     LEYPNGKTPPTDFHDAWRWFDSTLKGVSNGISEQPAVVYYVMGDADDPQAPGNVWRQA
                     DRWPPVPSKPTPFYCAADRTLSRSVPTAPSAWLSYRYDPAQPVPTLGGPQLTIPAGPK
                     DQRSIESRDDVLVFSTEPLDTPIEVTGRVRVKLWASSDAPDTDFFARLCDVYPDGRSM
                     NVCEGQLRARFRKSFAREKLLKPGRLYPFAIDLGSTSIVFNRGHRVRLHLTSSSAPGF
                     DPNPNTGEPFRASARRQAAQNTVFVEARHPTCIILPVVSN"
     gene            complement(9269..10357)
                     /locus_tag="PKK20_03085"
     CDS             complement(9269..10357)
                     /locus_tag="PKK20_03085"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNU98903.1"
                     /translation="MRHHFLAQLRESRRGAVTPDNTKEPARASMHALLAADQPILALA
                     PMQDVTDLAFWRLVHAYGGADLYVTEYFRVYPTSTLDRTILRAVTENLTGCPVIAQVI
                     GNDIPALVRTARQLQAYPIAGIDLNLGCPAPVVCRKRAGGGLLREPERIDAILAALRD
                     ALTTRLTVKARIGFDSPALFDDLLGVLARHRLDLVTVHGRTVTELYRGQVRYDLIARA
                     VRRLPCPVLANGNVSSAAKALRILEATGARGVMIGRGAIRNPWIFEQIRQARRGESVR
                     LPTGRDVLAYLEGLLDATRVPGMRERPHIQRVKKAATFVGLGIDPAGDFLHQIRRVTT
                     EADFHRVCRAFLDHDEPMPLEPFPLPLK"
     gene            10388..11311
                     /locus_tag="PKK20_03090"
     CDS             10388..11311
                     /locus_tag="PKK20_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98904.1"
                     /translation="MLRALAVSCVVVWFTTLVWAADPAARNNLAAQALLPQGPGIAIA
                     SGGDAGLRAHPDVIFADDFETGKLGENWDEVGNKEGRVLSWVDPRTALLGARCLRVEA
                     HLDKDTGGGLTKWFEPADTVFIRFYTQFDGACDYVHHFVTLRANRGLRGGDKWSGFGG
                     AGLKPAGDERFSTAIEPWGDWGRNPPPGRWNFYSYWHEMKVSPDGKYWGNSFAVPEAP
                     TIPRGSWICVEFMLKHNTPGQPDGEQAFWIDGRLLGHWKGINWRKTAQLRANALTLET
                     YVTDLWTKNPMNIVFFDSVVIARRYIGPAGE"
     gene            complement(11503..12564)
                     /locus_tag="PKK20_03095"
     CDS             complement(11503..12564)
                     /locus_tag="PKK20_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNU98905.1"
                     /translation="MTHHDQANTARCIDLRSDTVTLPTPAMRQAMAQADVGDDVFGED
                     PTVRRLEERTAELLGKEQALFMPSGTMANQVAVRAQTEPGDEVLIEAEAHIHYYEAGA
                     PAALSGVTCRCLPGRRGVFTPDDLRAALRPPDQHFPRPRLVCVENTHNRGGGSLWPLE
                     TLKAVAEAARAAGLRMHLDGARLWNASVATGVPERTYAALADSVNVCFSKGLGAPVGS
                     ALAGTRDFVARARRFRKQFGGGMRQAGILAAGALYALEHHRARLADDHANARALAEGL
                     ANLPGIDIDPAHVQTNIVLIRLTRLAASTVVHRLEASGVRVLATGPQTVRAVTNLNVT
                     RADIGSALAAFANAAGSPG"
     gene            12803..>13124
                     /locus_tag="PKK20_03100"
     CDS             12803..>13124
                     /locus_tag="PKK20_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU98906.1"
                     /translation="MSDMPTPASGVDNPVSSSRTDGGRDVAPTAVARGVYLGLERAIC
                     SVIRGQAAAVRRLLAAFASGGHVLLNDYPGTGKTTLAKSLARAMSARFTRIQFTPDLL
                     PSDIL"
BASE COUNT         2270 a         4349 c         4297 g         2208 t
ORIGIN      
        1 catctgccgc ttcgtgaacc cgcgcacggt ggtcttgatc cgcgagccca atcgccgcga
       61 catcaactac cggcccctgg ccgagaactg ggaacgcatc cgcgatgtcc ggctcgaaga
      121 cggctcgagg cccgaggtgg tgcctctgcc aatgccagcc ccgctctttt tcgaaaagga
      181 ccgtctgccg gccagctacg ccaatttcta tatcgccaat gccgccgtcc tcgtgccgac
      241 cttcaacgac cccaacgacc gaatcgcgct gggcatcctg gccgaactgt tcgccgaccg
      301 ccccgtggtg ggcatccacg cggtggacct ggttctcgga ttcggctcga tccattgcct
      361 cacccagcaa caaccgcgtt agaagtccaa cagacggagg gcgttgcggc cccgccgaag
      421 accaggtatt ccttcttcgc cttgatcggc agatgcaatc gatagggacg gcatctgccg
      481 atcgccgcca tggcccgctt ggccccccga tagagcttcg cacgggtctc ctccatcgaa
      541 cggtcacgtc aatcaggggt cggtctcggc ctcgagtcgc ctccgccacc ccgccatctg
      601 ccgatcactc gcccgcctca ccgccgccac cgcctgcgtc acccgcgatt cgtcccccat
      661 ctgcaggcgc cgactcaccc aggcccgcga cacaacggtc cgcttgcgca gccaccacgc
      721 caacacctgt ttctccttgg cccccttggc cagtgcggcc aagtctccct tcttcagccc
      781 catcaacgcc aatccctgcg ccagcagcac ctccgccgcc gcttcgtcgt gcgcccgacg
      841 cgcctcacct gcatgtgact tacgcgcttt cccggcaacg gccttctcga gccacttcac
      901 caaccgctgc cgaaaagccg gcccccccag gtaccacccg cgccgcaccc gcctccattc
      961 ctcctccaac gcctcccgac ttcgccggcg ccccagttcg agcacgcgcc cctccacgtg
     1021 cgcctcgtac tcctgccgtc cacgcgcgtc gtccgcccca atccccacgc tgcccagcac
     1081 gcgcttgacc tccagccacg gcggacgccc cgcccgcccc ctcaggtagt ccgggtaact
     1141 gctccatcga tacctgctca acgcctgctc cccgatccgg atcaggccgg cccgcgccgg
     1201 attcaggtga acgtacgtgc tcaccacctg gaaataccta tccgcacccc cgtccacaat
     1261 gagcgccttg taccgcccct gaaacaaatg cccgaacacg tcgttgcgtg cgttgtaccg
     1321 ctgcgtgtac gttccctgca gccacctcat ccccgccacc aggttgcctt ccggcgtctc
     1381 caccagcagg tgatagtggt tgggcatcaa cacataggca tgaatccgcc accccgtctt
     1441 ctcacaaccc tcggccaccg tctccagaaa cagcttccga tcccggtccc ctcgaaaaat
     1501 gaggccaccc tgattccccc ggctcatcac gtggtagacc gcgcccggat actcgattcg
     1561 caacttgcgc gccataacga acagcccgta cgcccgccgg gaggatgcgt caagaccaaa
     1621 gattcctgct ttccgggacc gacccctttt cggcaaggcc ctggaacggc ttgaaaagcc
     1681 gatcaacctc gactaagccc tttgtccatg acgactgaac ccatcaagtt ggaggatgcg
     1741 gtcgagttcg ccgagaaccc tgagccccga tgtccgtgcg tgttgctgct cgatacctcg
     1801 gggtcgatgc ggggcgaacc gattgcggct ttgagcgagg gcttgaaagc cttcaaggac
     1861 gacctcacca aggatgcgct ggcctcccgg cgagtcgagt tggctgtggt cacattcgac
     1921 agcgacgtga aggtggtgca ggacttcgtc accgtagatc aattcgagcc gccggaactt
     1981 gccgcccagg gccagacatt catgggcagc gccatccaca aggcgctcga tatgatccag
     2041 gcgcgcaagg cgaaataccg ggccaacgcc gtagcctatt accgcccctg ggtcttcatg
     2101 atcaccgacg gcgaacccca gggcgaaccg gacagcatca tccagcaggc ctcacagcgc
     2161 ctcaagagcg atgaggccaa caaacgcgtc gccttcttcg cggtcggcgt tgagaatgcg
     2221 aacatgagca gactggccga gctggttgtg cgagcgccgg tcaaactgat cggcctcaac
     2281 ttcgtcgaga tgtttgtctg gctttcgcgc agcatggaga aggtcgcgca atcgaagacc
     2341 gacgaacagg tggccctgcc gcctccgggg tgggggacgg tgtgaggggg aagtgcaaag
     2401 tgcagagtga agcatggcgc gtggcggcgg cgtcggtgcg ggggacaagc cacatcaagt
     2461 ccgacctgcc atgccaggac gcgttgcgct ggcagacggc acgcggcggg tggctgcttg
     2521 cggccctggc ggacggcgcg gggtcagcca gcctggctga aatcggggca ggcattgccg
     2581 ccgaccgggg catcgaggca ttgaaggcgc aggtcgaatg caagtcgctc gcggatgcgg
     2641 acgaagaatg gcgtgccggc gtggtggcgg cgctcgcaag cgcgcgcgag tcggttctcg
     2701 ccgctgccca ggaacgcaag gtcgaggcgc gcgaactggc cagcaccctc acgctgctgg
     2761 cggttgcgcc cgggatcgcc gtagcggcgc agattggaga tggcgccgcg ctcgcttcgg
     2821 cgccgcccga ggccttggcg tgcctcacca gacccatggt cgccgagtac ctgaacgaga
     2881 ccacgtttct cacttccgag ggcgcgctcg agtcggcgca gacggttgtg cggcgtggcg
     2941 cttggcgaca tgcggggttg ctgtgcgacg gtctgcaact gctcgcgttg cgcctgccgg
     3001 aggggacgcc gcacgcgcgt ttcttcgaac cgctctttgg ttttgtcgat cgagcgacgg
     3061 atctgacggc tgccagccgg gagttgagcg cgtttctgca atcgcagcgc atcaccgagc
     3121 gcacggatga tgatctcagt ctgctactgg cggcttatgg caactcgccc ggatcaggtt
     3181 gagcgcggag cgtgggactg atccgggcga gccgctgcgt gaatccaatc cgtgcgattc
     3241 caacgcgcat ccaacggcga cattgtgact ctggcggaga atcaccgcct gggagcggga
     3301 ggcgaagcgc gcatttacgc ggtgccggga gaaccgcgac tagtcgcgaa aatctggcac
     3361 aaaccaacgc ccgagcgcag ccgcaagctg cgcgtgatgc tagccaatcc gcccatggac
     3421 ccaatggcgg cgcatcacca cgcgtcgatc gcctggccga tcgatgtgct gagcgcgcct
     3481 ggccggcccc aggccctggc ggggttcatc atgccgcgcg tcgagaacat gcggcccgtg
     3541 ggcgagttca gcaatccgaa agtccgccgg acccgttgtc cgctgttcaa ctacgcatac
     3601 ctgtatcgga cggcgcgcaa cctggccacg gccgtacgtg ccctgcatga gcgcggctac
     3661 gtgatcggcg atctcaacga gtccaacgtc ctggtggcgg agacggccct ggtaaccatc
     3721 gtcgacaccg actcgttcca ggtttgggac gccgaggcgg gtgtgatgta ccgctgccgg
     3781 gtgggcaagc ccgagtacac cccgccggaa ctgcaaggca agagttttgc ccaggtcgac
     3841 cgcgaggccg cccatgaccg gttcggcctg ggcgtgttga tcttccaatt gttgatggag
     3901 gggacacatc ccttcgcggg cgtctaccgc gggcgcggcg aacccccgtt gctcgaacag
     3961 cggattgcgg ccagccattt cccgtacgcg cgcgaggcgc atctgtttta cgcgcccaaa
     4021 cccaccgcgc cccccttcga cacgctcgcg cccggtctgc gcgacttgtg tctgcggtgt
     4081 ttccaggacg gccattttcg ccccagcctt cggcccgagc ctcagagctg gcagtacgtg
     4141 atcgaggagg cggagtcgca cctcgtcacg tgccaatgga actcccagca cgtctttggc
     4201 gatcacctcg aacagtgtcc gtggtgcgag cgggcagaac agcttgccgg gcgcgacccg
     4261 ttcccctcga tcgagggggt gaaacgggga gagcaccgtg cgccgccccc atgggcccgg
     4321 gagcgcggcg gggcgcgcag cggtcaacgg cgctcgccct tctggccgac gggcatgccg
     4381 aatattccca aacccccgcc catcccgcct tccgcgcctc gcgcgagaac ctcgagacga
     4441 cgccgtccgc gtccattgct tggcgactgg aacgattggg cgtgggtggc cctagcctcg
     4501 agcggactgg ccgcgggcgc cgcggcctcg tttcagtggc aggccaaccc gctgacattc
     4561 ctatgcggca tcttcgccct cttgacgggt attctcggtg aagccaaggc ccggagcccc
     4621 gagctggatg gacggggcaa gtggatcgcg cgcaccgggc tggcgcttgg cacaggggcg
     4681 ttggtctggt gcctgacggc cacggtgtga ggcgcctcga attatccggg tggctccaga
     4741 tcgtggaaat cgacctcgag gtcggttgcg cccacccgca ggatcgctac gctgcggggc
     4801 gcgtcgaatc gccgcctgcc cgccgagccc ggattgagaa agagcgtctc gccaatccgc
     4861 tcccggaacg gtttgtgggt gtgcccgaag accaccacat ccggttgcac ccggtcgatt
     4921 tgacgccgca gggaatccgc cagccggcgc ggctcgacca cgtggtgcac gaggaacctg
     4981 cgcccgaaca gctcgatcac ctcggtctcg cgcaacgaga ggccggagtc ggcatttccg
     5041 atcaccgccg tcaccggggc gatcgcttcg agttccatga tgatccgcgg caagccgatg
     5101 tctccgccgt gcaggatgtg atccacgccg gcaaagagag acttgacctg aggatccagg
     5161 aagttgtgtg tgtccgagat aataccgagc cgggccatta cgctttggct tcgccgggct
     5221 cgggggcctc ctcgggctcc tcggacggtt ccttttcctc ttcgggcttg ggctcgaccg
     5281 cctcgcccag cttcatggcg ccggcaaaaa ggcctgcgaa gagcccgccg ctgagcaggg
     5341 tgttgcggaa gaactcccaa gtgtgcggcc atccgggcgt gccgacagtc agagactgta
     5401 tccagccggc gagattcttg gcataggcgg ggtcgtgcag ccacgagacc gtgttcgaga
     5461 caatgtagaa cagaatggcc cccagaatcc ctcccccgaa cagcgtcagc catgacgagc
     5521 gccggttgaa ttggcgcccg agccagacga tgccggcgta cgcggcgtag ttgcccagca
     5581 tctcccattg cagcggggcc acgccatagt ggacgttcag cagcacatcg gtgaccagca
     5641 cggtgcccag cggcagcacc cacgccaccc agccggagaa ataaaccgcc gcacagaaca
     5701 acagcccgta gacaggactg aagttcggcg gcaacagccc gggaatccgg gtcagcgcca
     5761 gaaccgccag caacaccaaa ggtaagccgt gcttagcttt cacactgcgt cagcatacgg
     5821 accggccgcc agatgacaag cgcgatgctg aggacggtcg cgccgcgagg ttgacgcagc
     5881 attggccgcc cggaacgcgg cagtcaagat tccatctcac gcgaagcgcc tcaggctaca
     5941 acgcccagcc ctcgcgatag gcgcggccga gcagctcgtt cgcggacgcg tcattgagga
     6001 tgcgcaggtt ggggccgtcg tagaggagtt tcttctgctg cacccgcgcg gccacattgc
     6061 ccaacaaaca gacctcggtc accagccccg cgtgatcgac aaaattcgag ccgggcggcg
     6121 ggccgccgcg gattccggca acccactcgt gatagtggcc gggagagcgg ggcagcgtct
     6181 tgggcggcat gcggtaggtg tccatgcgcg aatcgggcag caggcgttgg cccatcaact
     6241 tgccctgatc gccaatgtaa atcgtgtcct cgatgccatc gttcggatcg agttcgggcg
     6301 gacgcggcgg tttcaacccg ccgtcggacc atgtcagcac cacgggcggc atggcgccgc
     6361 gcgcgggaaa ctcgaagcgg acgatttcgc cgagcgggta ggtttctggc gagagcttgg
     6421 tggcgctggc ctcgacgctg gcggggtgtc cgagcttgag ggccttgaac accgtcgaca
     6481 acttgtgcgc gcccagatcc cccagcagcc ccgtgccgaa atcgatccaa ttgcgccagg
     6541 tccacggatg atacgccgga tggtatggac ggtccggcgc cggaccgagc cacaggtccc
     6601 agtcgagccc ctccgggacc ggcggcgtct ctggcggacg tccgtcccac gcgggccagg
     6661 tccagaaacg cgcccccgac cacacatgca cctcgcgcac cgcgccgatg gccccgtcgg
     6721 caatgatctc ctgcacgacg cgcgcgccct cctcggcctg gccgtgattg cccatctgcg
     6781 tgaccacgcc cgcttggcgc gcggcttcgg cgacctggcg ggcctcgcga accgagtaag
     6841 tcagaggctt ctcacagtag acatgtttgc ggcgtttcag cgccgccatc gcgatcacgg
     6901 catgggtgtg gtctggcgtg gcaatcgtca ccgcgtccac atcctccttc tcgagcagtt
     6961 cccggtaatc cacgtaggcc gcgcaacccc gatactggcc cgagccccgt tgcgcggcgt
     7021 agcccgactc gaccgcgcgc cgcgccggtt cccgtccgca tgtccgttcc tccctccctt
     7081 gcgaccagtt ccaggaaagg tagcgattgc tctcgcgctc gacgtcgcag acggccacca
     7141 cttggatctc ggggaacttc tggagcgcgg caagattctg catcccctgg ccgcccgtgc
     7201 cgatgcccgc gagcgtgatc ttgtcactgg gcgcgatttg gcccgcaccg cccagaacgt
     7261 gccggggcgc gagggtcagc ccggtgacgg cggcagcgga aaggtgcaga aactggcgtc
     7321 ggttcggcgc ggagcaggag ggttcggggg gaatcggacg attggatcga ggcaaacagc
     7381 aaacggaatt catggtcaac catttggcga ttcaaccgca tttgccaaga agattcgcga
     7441 ctcaattcga gacgaccggc agaatgatgc aggtcggatg ccgggcctcg acgaagacag
     7501 tgttctgggc tgcctgccgg cgcgcgctcg cccggaacgg ctcgcccgtg ttgggattgg
     7561 gatcgaagcc gggggcgctg ctcgacgtga gatggagccg gacgcgatgg cctcggttga
     7621 agacgatgct tgtcgacccc agatcgattg cgaacggata aagccggcca ggcttgagca
     7681 gcttctcgcg agcgaacgat ttccggaacc gcgcgcgcaa ttgcccttca cagacattca
     7741 tcgaccggcc gtcgggatag acatcacaga gccgggcgaa aaagtcggtg tccggggcgt
     7801 cactcgatgc ccaaagcttg acccgcacac gcccggtgac ctcgatgggc gtgtccaacg
     7861 gttcggtcga gaacaccagc acatcgtccc ggctctcgat cgaacgctga tctttcggcc
     7921 cggcggggat cgtgagctgc ggtccaccca aagtgggcac cggctgcgcc ggatcgtacc
     7981 ggtaggagag ccaggcagac ggggccgtcg gcacgcttcg ggacagagtt cgatccgcgg
     8041 cgcaatagaa gggcgtcggc ttcgagggca caggcggcca gcggtcggct tggcgccaga
     8101 cattgccggg cgcctgcggg tcgtccgcat cgcccatgac gtagtagacc acggcaggct
     8161 gctcggagat gccattggac acgcctttca aggtcgaatc gaaccagcgc cacgcatcgt
     8221 gaaaatcggt cggcggcgtc tttccgttcg gatactcgag atcgccggcc ttgtcctgga
     8281 acactatatg ggtccaggga cccatgacga gcctttgccg ccctttggct cgaggcccac
     8341 cccgctcctg gtagcccaca aatgcgtcga tcgagccctg cgcaaagatg tcaaaccagc
     8401 cgccaatatg gaccccggcc gcattcgctt tcccatagcg ccgatccaac tgccgcgcgc
     8461 gccagtatgt gtccagacgc ggatggctgg tccagagggt cagggcgtcc ggactgaact
     8521 tcgagatgcg cagccagtcc tcgaccatcg ctctcttgaa cacgccgccc gggtaaacgc
     8581 agtcgcgata gaggttgggc gcgcccacag tgatgtgctg cgacgtgatg cgcgaggacc
     8641 cggcaccggc cagcagcaac tgcgtgatcc ccacagccga accgccgtat gtgccgatct
     8701 tgccgttgca ccagcgctga cccgacagcc actccgcggt gtcgaaaccg tccgccgcgc
     8761 cgtcccagcc gtccgtcgcg aacggcagat tcggcccttc ggaaccgaac cgcccgcgcg
     8821 tgtcctggac cacgatggcg tagccacgac gggccccctc ggcgccgatg ttgccgacat
     8881 tctccttgtt gtacggcgtg cggatcagga tggcgggaaa cggaccttcg ccctcgggca
     8941 ggaagacgtc cgttgccagg agcacgccgt cgcgcatggg cacctcgagc gtctgcttcg
     9001 gcggtggtgc ggcttcgatc cgaagaatcc atacagtgca gagcgccagc cagcccagac
     9061 cgcccgcgga ggttcgagga gaattcatgc gcgaaccatg ccggcccgat cgcatttggg
     9121 gaaggaggtc acatcttcat atttgacatt ccggctgaga tggcgcggac cgtcggaccc
     9181 gtgttgcggg gtcccctggg cgcatggcct gacgggattg catcggcgcg atacgaaggc
     9241 aattgtcaaa tatgaagatg tgaccccgct acttcagggg gagcgggaac ggttccagcg
     9301 gcatcggctc gtcgtgatcc aggaaggcgc ggcagacgcg atggaagtcg gcctcggtcg
     9361 tgacacggcg gatttgatgg aggaagtcgc ccgcggggtc gatgcccagg ccgacgaacg
     9421 tggcggcctt tttcacacgc tggatgtgag ggcgctcgcg catgccaggc acgcgcgtgg
     9481 cgtcgagcag gccttcgaga taagccagca cgtcgcggcc cgtgggcagc cgcacggact
     9541 cgccgcgacg cgcctggcgg atttgttcga agatccaggg attgcgaatc gcgccgcgac
     9601 cgatcatgac acctcgggcg ccggttgcct cgaggatgcg gagggccttg gcggccgagg
     9661 agacatttcc gttggccaga acggggcagg gcagacgccg gacggcccgg gcgatgagat
     9721 cgtaccgcac ctggccgcgg tagagctcgg tgaccgtgcg cccgtgcacg gtgaccaggt
     9781 caagccggtg ccgggccaac acgccgagga gatcgtcgaa cagcgccggg gagtcgaatc
     9841 cgatacgggc tttgaccgtg agccgggtcg ttagggcatc gcgcagcgcg gcgaggatgg
     9901 catcgatgcg ctccggttca cgcagcaggc cgcctccggc ccgctttcga cagacgaccg
     9961 gcgcgggaca gcccaggttg agatcgattc cggcgatggg ataggcctgc aactggcggg
    10021 cggtgcggac caaggccggg atgtcgttgc caatgacttg tgcgatgacg gggcatcccg
    10081 tcagattctc ggtgacggcg cgcagaatgg ttcggtcgag cgtcgaggtt ggatagacgc
    10141 gaaagtactc tgtcacgtag aggtcggcgc cgccataggc atgcaccagg cgccagaacg
    10201 cgaggtcggt gacatcctgc atcggcgcca gcgcgaggat cggctgatcg gcagccagca
    10261 gagcgtgcat cgaggcgcgc gccggttcct ttgtgttgtc cggtgtcacg gcgcctctcc
    10321 ggctctcgcg caactgggca aggaaatggt ggcgcatcga gacgcggatg gtattgttgg
    10381 tttcatcatg cttcgcgcgc tcgccgtttc atgtgtggtg gtttggttca cgacgcttgt
    10441 ctgggctgcc gacccggcgg ctcgaaacaa tcttgcggcg caggcgctgc ttccgcaagg
    10501 cccggggatt gccatcgcgt cgggaggaga tgccggcctg cgcgctcacc cggacgtcat
    10561 cttcgcggac gatttcgaga cgggcaagct gggcgagaac tgggacgagg tcggcaacaa
    10621 ggagggccgg gtgctcagtt gggtcgatcc tcgcacggcg ctcttagggg cgcgttgtct
    10681 gcgggtcgag gcgcatctgg acaaggacac tggcgggggg ctgaccaagt ggttcgagcc
    10741 ggcggacacg gtgttcatcc ggttctacac ccagttcgac ggcgcgtgcg attacgtcca
    10801 ccacttcgtc acgttgcgag cgaaccgggg cctgcgcggg ggcgacaaat ggagcggatt
    10861 tggcggggct gggttgaaac cggcgggaga cgagcgtttc tcgacggcga tcgagccgtg
    10921 gggggactgg ggaaggaatc ccccgcctgg ccgctggaac ttttacagtt actggcacga
    10981 gatgaaggtg tcgccggacg ggaaatactg gggcaactcg tttgccgttc cggaggctcc
    11041 aacgatcccg cgcggctcgt ggatctgcgt cgagttcatg ctcaagcaca acaccccggg
    11101 ccaacccgat ggggagcagg cgttctggat tgacggcaga ctcctgggtc actggaaggg
    11161 gatcaactgg cgcaagaccg cccagctcag agcgaacgcc ctgacactcg aaacctatgt
    11221 caccgatctc tggacgaaga acccgatgaa catcgtcttc ttcgacagcg tggtgatcgc
    11281 gcgccgctac atcggccctg ccggggaata gccctggagg tcagcgcggg tccggaactg
    11341 tgttcgagag cgggcgcggg ccggctggtt cgttccgcgg tcggtcgagc acctcagggg
    11401 atacacttcc ttcctcgggc ggctcgaccg agctcgccga ggccctcgcg tctggcccgg
    11461 tggcgctccc gctaaaaccg ggattagttt ttggacaggc ccttatcctg ggctgcctgc
    11521 ggcattcgca aacgcggcca gggccgagcc gatgtcggct cgggtcacgt tcaggtttgt
    11581 gacggcccga acggtctggg gacccgtcgc caaaacccta acgccggacg cttccaggcg
    11641 gtgcaccacc gtcgatgccg ccaggcgtgt caggcggatc aacacgatat tggtctgcac
    11701 gtgtgccgga tcgatgtcga tgcccgggag gttggcgagt ccttcggcca acgcgcgcgc
    11761 gttggcgtgg tcgtcggcca ggcgggcgcg gtggtgttcg agagcgtaca gggcgccggc
    11821 ggcgaggatg ccggcctggc gcatgccgcc gccgaattgt ttccgaaacc gcctcgcccg
    11881 ggcgacgaag tcgcgcgtgc cggccaatgc cgagcccacc ggcgcgccca ggcccttcga
    11941 aaagcagaca ttgaccgaat cggccagcgc cgcgtaggtc cgctccggaa cgccggtggc
    12001 gaccgaggcg ttccagagac gcgcgccgtc cagatgcatt ctcaggccgg cggcgcgggc
    12061 ggcttcggcc acggccttga gggtctcgag aggccacaga cttccgcccc cgcggttgtg
    12121 ggtgttttcg acacaaacca ggcgcggtcg cggaaagtgt tggtcgggcg ggcgcaacgc
    12181 ggcgcggagg tcgtcggggg tgaagacgcc gcgtcgtcca ggcagacagc ggcatgtgac
    12241 tcccgacaat gcggcggggg cgccggcctc gtagtagtgg atgtgggcct cggcctcgat
    12301 gagcacttca tcgcccggct cggtctgcgc acgcacggca acctgattgg ccattgtgcc
    12361 cgagggcatg aagagcgctt gttccttgcc gagcaactcg gcggtccgct cctccagccg
    12421 ccgcacggtc ggatcctcgc cgaagacgtc gtcgccaacg tccgcctgcg ccatcgcctg
    12481 ccgcatcgcc ggggtcggca aggtgaccgt gtcgctgcgc aaatcgatgc accgagctgt
    12541 gttcgcctgg tcgtggtgtg tcatgactgg cctcgatcgt agccggtcgg ggcatggttg
    12601 gcgacggcca tcttcagcca cagattgtac tgatgagaag acagggtcaa cagccaacgt
    12661 gaatcgcaag ctattctgat cccgcaaggg cccacggttt ggggtgcccg gcttcgtggc
    12721 ggtcacgacc ccgtgaagca tgcgggcaag ctgttgcacg ttccacattg cgcgcggggt
    12781 ggcccgtggc tagtctccgc gcgtgtccga catgccaacg cccgccagcg gagtcgacaa
    12841 cccggtgtcc tcgtctcgaa ccgacggcgg ccgcgacgtc gcgccgactg cagtggcgcg
    12901 gggggtttac ctcggtctcg agcgggcgat ttgctcggtg atccgggggc aggcggccgc
    12961 ggtgcgccgg ctgctggcgg cgttcgccag cggcggccat gtcctgctca acgactatcc
    13021 gggcacaggc aagaccacac tggccaagtc cctcgcccgc gcgatgtcgg ctcggttcac
    13081 ccgaatccag ttcacgccgg acctgttgcc atcggacatt ctcg
//
LOCUS       DAOQRO010000048        12930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3926_length_12930_cov_5.693825, whole genome shotgun sequence.
ACCESSION   DAOQRO010000048 DAOQRO010000000
VERSION     DAOQRO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3926_length_12930_cov_5.693825"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="PKK20_03105"
     CDS             complement(<1..120)
                     /locus_tag="PKK20_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNU98907.1"
                     /translation="MRAKGTKGVARFSISLPGELAEQLDRMTREKGYDNRSLAI"
     gene            complement(272..1090)
                     /locus_tag="PKK20_03110"
     CDS             complement(272..1090)
                     /locus_tag="PKK20_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNU98908.1"
                     /translation="MIDLVFWAPVMTGGALAGASSGVLGTFIVGMRIPFLGVCVAHAA
                     LAGAVFGGLAGLEGQQLLLPALAGAVLTALALGLLNPQRAHLDDNTVLSFLFSATMGL
                     AFLGIGLYAIMGRSDNDVRSLLWGSLNFCRWRDVRLMLAASTALAIYVVLFFKELRAI
                     LFSRADAEASGIRATAVWTGFLILTAATLTVNFQTVGGLMIYSLICNPAAAAFQLTRG
                     CGPVLALAAVFGAASGLGGFLIAAGTDLPSGAVIVLVSSALLGVAAMIGHRGRR"
     gene            complement(1083..1886)
                     /locus_tag="PKK20_03115"
     CDS             complement(1083..1886)
                     /locus_tag="PKK20_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNU98909.1"
                     /translation="MTRAAVEVRSLVVRAGHRWMLDIEHLRVDLGSVVALMGPNGAGK
                     TTLLRACLGLARPARGSCTVLGVDVGGLHGPGLASLRRRIGYVPQLLPVRSELPLTVR
                     EVAAIGRTAHAGLFHALTGEDWRVVNGWLERLELTALADRAFSQVSGGEQRKAIIARA
                     MAQDPELLLLDEPTANLDLGWRERIVDTVQSLHTQTRISVLLVCHELEVLPPACRKVI
                     LLEGGRVSASGSPETVFTTARVSQLYGAGLRAVHEGGRHAVVPGGSAHD"
     gene            complement(1883..2716)
                     /locus_tag="PKK20_03120"
     CDS             complement(1883..2716)
                     /locus_tag="PKK20_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNU98910.1"
                     /translation="MIRPLLRMCATAALGLLILPSCRRAAPPADVPRIATTTSYLEAA
                     ASDLLGDTLSIVRLAEPGTCPGHFDMRPSQIAALRQCRALFRFDFQKGLDAKFHGADD
                     PLRVTSVSLPRGMGRPDSYLLACRQMADALSALELVSRTEAETRLREIADRLDALSRD
                     ATNRVRGAGLRGVAVIASVRQRDFCEWLGLDVVATFSAADAAGIGEIEQAIAAGKLAS
                     VKLVIANLPEGRRTADALAERLEAGVIVFENFPLVRNGRVSFDAMLEANVDALARAAL
                     P"
     gene            complement(2713..3360)
                     /locus_tag="PKK20_03125"
     CDS             complement(2713..3360)
                     /locus_tag="PKK20_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU98911.1"
                     /translation="MRAANQRSAGFTLVELLVVMAVIASLAGLLLPALARAKEKAKVI
                     KVHAELYGVGLALEMYSDDHGGQLPPVRVDCNSDLSAHWCQFPVELAQEGYLARGSKP
                     GLAANMQDAFNPGHTYKYATPGPQLLNGSAGGNYRLWVPDDFPNCASASGKYYSNRID
                     SPVRWVLWSLGPRPDSDRTQDSHAPITSRSWYRRAGEGGVIARIATRETMQFKTP"
     gene            complement(3369..4538)
                     /locus_tag="PKK20_03130"
     CDS             complement(3369..4538)
                     /locus_tag="PKK20_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98912.1"
                     /translation="MKSKMVMSFESAARQIAMAIAFCAVAAPALAQTETHAALQAVNS
                     VGASTWNGSFPFTLRGVLLCNPDEMLDATPQFLPWANGANQYRMGGEWQVAFQAVDPG
                     DRGGTMCWMGQNYGNQPWIHESDLSYDNEAWVREVLRLSFDPSSLRQYRAGDLIEVTA
                     RQALFYGGKRNINEGHDIDPAFDFNIRLVTPNYGLPAPEVITLADVMLPGGSADDPAS
                     WPAIFDPTRATGGEHYQGMRVRLNNLTLVTTNGWNPTNSWAMRRCTVTDNEGRFFWLR
                     HPRFSLGAAPAGRFDAVGIFNQESGSGSQGTNGYELFVQQVIPQEPPVLGIANTVALS
                     WPVSGAAYQLESRADVNSTEWSAVTNRPAEIEGRNTVLVPPAPDPHQVYRLRKTN"
     gene            4674..5339
                     /locus_tag="PKK20_03135"
     CDS             4674..5339
                     /locus_tag="PKK20_03135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU98913.1"
                     /translation="MNRAHGSTPARDPRIAYFDRQAPTWDRTGPDPDATMRRLRDLDG
                     RLGLKAGLDLLEVGCGTGQITGWLADTVRPGRVVAVDFSEAMLAQARARGVDAKFVLL
                     DICGEEPFEGSFDRVLCFHAFPHFRDQAAALRRMARHLKPGGQLLVLHLSGSRQLNAF
                     HQSLGGAVGHDHLPPAARWPALLRPVGLEVREAVDRADLFLLKAEAGARGGTARQDSC
                     IPH"
     gene            5345..6943
                     /locus_tag="PKK20_03140"
     CDS             5345..6943
                     /locus_tag="PKK20_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNU98914.1"
                     /translation="MKSKLCLLGGLMLLAIVPLRAADSVRPNILFIFSDDHAEHAISA
                     YGSKVNQTPHIDRLARDGVRFLNSFVVNSICTPSRATLLTGQYSHVNGVPVFNRFDGA
                     RDHVAKRLQAGGYHTGMIGKWHLGSDPTGFDRWIVLPGQGAYWNPAFLVSGRQLTIQG
                     HCTDITTDLGIEWLKTRPQDKPFFLMLHQKAPHRAWEPDERNKALFQNKVIPEPDTLW
                     DDYATRPAALPRNRQTIARDLTRRDLKLEPPSDLKGPARNQWLQEVPMELSVDGKTLT
                     GKDLVRWKYQRYMQDYLACVQGVDDGVGRILDYLDQVGLAANTVVIYSADNGWYLGDL
                     GLYDKRFMYEPGLRVPLLARGPGLQAGHTPSQLVANIDLAPTFLDLAGLPVPESMQGR
                     SLVPLLRGAAPSDWRSSVYYRYYHDPGHHNTAAHLGVRTATHKLIYYWKQDAYEMFDL
                     VKDPAEQHNLLHSPVEAQRSEVAARFAELKAELVRLQKEFKDDGRYADPATWPSGSSD
                     GPFGDKQPVGIKTVAEAIALTSAE"
     gene            complement(7006..11349)
                     /gene="hrpA"
                     /locus_tag="PKK20_03145"
     CDS             complement(7006..11349)
                     /gene="hrpA"
                     /locus_tag="PKK20_03145"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNU98915.1"
                     /translation="MNPVASLKELERLLPRCLLTDQLRIGWKVTRWLEAHRQSSRAPH
                     DLPRWLDEARDSIARRARRQAALPELRYPHELPISAKRNEILDALRAHQVLIVAGETG
                     SGKTTQLPKICLEAGFGVRAKIGCTQPRRVAALSLSRRVADEIDRPWGRAVGCKIRFS
                     DQTSPETYVKFMTDGILLAEAQADPVLSEYEVVILDEAHERSLNIDFLIGLLKRLLSQ
                     RSDLKLLVTSATIDTEAFARHFDDAPIITVSGRLFPVTVEYRPFGAGSGDEDGDRTED
                     GRTYVDAAVDAVQEILATSASGDVLVFMPGERDIIETRDALREAGGGRLELVPLFGRL
                     STDEQQRVFAASERRKIVIATNIAETSLTVPGIRYVVDTGLGRISRYNARTRTRRLPI
                     EPISQSSANQRKGRCGRVAEGVCYRLYSEEEFQARPLYTQPEIQRANLADVILRMKAF
                     DLGEVEEFPFIHPPLPAAIAGAYQLLAEIGALDADRRLTPLGERLARLPVDPTIGRMI
                     LQAQIEGVLEPVLIISAGLSIQDPRERPADRQDAASAAHRRFQHPRSDFLTLVRIWEA
                     CHESLESLKTQSQMRKFCREHFLSFPRMREWRDIHAQLRETVAHMGGPPGPAAPDDAD
                     ETLEDALTQGPHTLASNEPCPRPAVRDESAPSRSVKGRNQRHLTTPAAGFIAQSEQQQ
                     TAGRGADSWYAAVHRSILTGLFSHVAARKERNLYRLAGNREAMVFPGSGLFERGTERR
                     VESGHKDAPKAPPRSQTQPAWIVAGEIVETSRRFVRTVAEIEPRWILDLAPHLIRKAH
                     LDPHWDPTAGRVAALERVTLNGLVVSERMVSYRQSHPAEATEIFIRAALISEGLADHF
                     RSATRVQDNAAQSLTARAGGAGQGRREQAALGQSRVDPPPPDLASLPSAYQFLRHNHQ
                     LRQKIELWQSRLQHRLVPDLDEALHRAYAKRLSNVGSVADLNHLLRERRAEGTSWLCL
                     DAADLLGKHAGAFDGLAFPDAVAIGTQQVPVAYDYAPGDDRDGVTVRLPFTLAQVIEP
                     HLLDWAVPGLREPQILHLLQALPKDLRRPLMPLAPRAKEMAQAIKPDGSAFLPALIRF
                     IRERYGVEVPSTAWSVELLPAHLRPRFEILGRDNQPLACGRDLRALQVELESHDTTIE
                     SEAWRSAVARWERYGLKDWTCGDLPDRIQVADVAGFPIEAFPALHVEAGEVSLRLFRT
                     AAAAIEAHAGGVRRLLEMGLQRELAWLAKDLRGLNQWRLLYITIGPVDELEATAFANL
                     RRHLLTASAASPRTAAAFAALRESTRAAIRGLAPQLSDRLAEILRLRQAILLCRKPYP
                     RMREDLDRLVPAGFLGQVPFDRLPHLARYLKAMLIRAERAAVNPAKDREKLDRVQPWI
                     AALAAIRIAPDASDAVRGERDALRWLIEEYKVSVFAQELGTAGTVSPQTLEQRLAALR
                     NRPASNTVRSR"
     gene            11829..>12930
                     /locus_tag="PKK20_03150"
     CDS             11829..>12930
                     /locus_tag="PKK20_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98916.1"
                     /translation="MKACQVAMSLAVATGWLAVSSRAAETELRPVVEVEEVIYEYTPA
                     DNGAGPMWCHGSTCLVRLGDAVFASGLETIPHEKPLNNCRWTFWRRGPEGWTLLRQDS
                     VGRTREPCPLAAFPEPGRVFLSANPTLNPPGQAGGGPARPVLFEFMAADASAPPGSHW
                     PGWECKPAPPAFTEHSYRSLAADGPGREMILFQNIGYTHAEWAFRDRDGNWSAQGRLK
                     WPWGAEYDTPQPIRVCYPNVALRARAVHFVGVSDIVEPYAKWRQAKRELTGQDWDYDF
                     RRLFYVWSPDIAHGGFGDWVEIASRDKTCGWISPGDLWVAPDDAVHIVWTERALDERL
                     RPKFFPNERQRHELNYARLREGQIETRRTLLAV"
BASE COUNT         2226 a         4245 c         4302 g         2157 t
ORIGIN      
        1 gatggccaac gatcggttgt cgtaaccctt ctcgcgggtc atgcggtcca actgctcggc
       61 caactcgccc ggcagtgaaa tgctgaagcg ggccacgccc ttggtgcctt tggctctcat
      121 ggcgggggac cttaccagat gccaggtggg cgtcaaatgg cgcgtcaacc ggcggtgatt
      181 tcacacgctt cgcggggact cctaacccgc atggcggtcc tcttgcgaag tctgaccgcc
      241 atgcgcaccg acagggtgtg aaatgtgcgg gttagcgccg ccctcggtgg ccgatcatcg
      301 cggcgacccc gagcagggcc gaggacacca gcacgatcac cgcgccgctg ggcaggtcgg
      361 tgcctgccgc gatcaaaaaa ccgcccaggc cgctggccgc gccgaacacc gccgcgagcg
      421 caagcaccgg cccgcagccg cgcgtcaact ggaatgccgc cgcggccggg ttgcagatca
      481 ggctgtagat catcaggccg cccactgtct ggaagttcac cgtcagcgtt gcggctgtca
      541 ggatgaggaa gcccgtccag acggctgtgg cgcggatgcc tgacgcttcg gcgtcggcgc
      601 gggagaatag gatggcacgc agttctttga agaagaggac gacgtagatg gccagggcgg
      661 tcgaggcggc cagcatcagg cggacgtccc tccaccggca gaagttgagg ctgccccaga
      721 ggaggctgcg cacatcgttg tcgctcctgc ccatgatggc gtagagcccg atgccgagaa
      781 aggccagtcc catcgtggcc gagaagagaa agctcaagac cgtgttgtcg tccagatggg
      841 cgcgttgggg gttgagcagt cccagcgcca gggcggtcag caccgcgccg gcaagcgcgg
      901 gcaggaggag ttgctgtcct tcgaggccgg ccaggccgcc gaagacagcg ccggcaaggg
      961 cggcgtgggc tacgcagacg cccaggaagg gaatccgcat gccgacgatg aaggtgccga
     1021 gcacgccgct cgaggcgccg gccagggcgc ctccggtcat cacgggagcc cagaacacga
     1081 ggtcaatcat gggccgatcc tcccggcacc acggcgtggc gtccgccctc atgcaccgcg
     1141 cgcaggcccg cgccgtagag ttgcgacacg cgcgcggtgg tgaacaccgt ttcgggcgag
     1201 ccggacgccg aaactcgccc gccttcgagc aggatgacct tgcgacaggc cggcggcaga
     1261 acttccaact cgtggcagac gaggaggacg ctgatgcgcg tttgggtgtg gagagactgc
     1321 accgtatcga caatgcgctc gcgccagccc aaatccaggt tggccgtggg ttcatcgagc
     1381 aggagcaact ccgggtcctg cgccatggcc cgcgcaataa tcgccttgcg ctgctcgccg
     1441 cccgacacct ggctgaaggc gcggtcggcc agcgccgtca actcgaggcg ctcaagccat
     1501 ccattcacaa cccgccaatc ctccccggtc agcgcatgga acaggccggc gtgcgcggtg
     1561 cggccgatgg cggccacttc gcgcaccgtc aacggcaact cgctgcgcac cggaagcaac
     1621 tgcggcacgt agccgatccg gcggcgcaga ctggccagac cggggccatg cagcccgccc
     1681 acgtccactc cgagcaccgt gcagctgccg cgcgcgggac gcgccaggcc gagacaggcc
     1741 cggagcaacg tggttttgcc cgcaccgttc ggccccatga gcgcgaccac gctgcccaga
     1801 tcgacccgca gatgctcgat atcgagcatc cagcgatgtc cggcacggac gacgaggctg
     1861 cggacttcga cggctgcgcg cgtcatggaa gcgccgcgcg cgccagggcg tcgacattcg
     1921 cttcgagcat ggcgtcgaac gagacgcgtc cgttgcgcac cagggggaag ttctcgaaca
     1981 cgatcacgcc cgcttcgagc cgctccgcca aggcatcggc cgtcctccgg ccctcgggga
     2041 ggttggcgat gaccagcttg acggaggcca gcttgcccgc cgcgatggcc tgctcgattt
     2101 caccgatgcc ggcggcgtcg gcggcactga aggtggccac gacatcgagg ccgagccatt
     2161 cacagaagtc gcgctgccgc acgctggcga tcacagccac gcctctcaat ccggcgcccc
     2221 tcacgcggtt tgtggcatcg cgggacaagg cgtcgagccg gtccgcgatt tcccgcaggc
     2281 gtgtctcggc ctcggttcgg gagaccagtt cgagcgcgct cagcgcatcg gccatctggc
     2341 gacaggccaa aaggtagctg tccggccgcc ccatcccgcg cggaaggctc accgaggtga
     2401 cacgcaaagg gtcgtccgcc ccatgaaact tcgcatcgag gcctttctga aagtcgaatc
     2461 ggaacaaggc gcggcactgg cgcagggcgg cgatctggct ggggcgcatg tcgaagtgcc
     2521 ccgggcaggt gccgggttcg gcgagtcgga cgatgctcaa ggtgtcaccc agcaggtcgg
     2581 acgcggcggc ttctaggtag gaggtcgtcg tggcgatgcg cggaacatcc gccggcggcg
     2641 cggcccggcg gcagctcggc aggatcagca gtcccagcgc ggctgtcgcg cacattcgaa
     2701 gcagcggccg gatcatggcg tcttgaactg catcgtctcg cgcgtggcga tgcgggcgat
     2761 gacgccgcct tcccccgcgc gccggtacca actgcggctg gtgatcggcg cgtggctgtc
     2821 ctgggtcctg tcactgtccg gacgcggtcc caggctccag aggacccacc gcacggggga
     2881 gtcgatccgg ttcgagtagt acttgccgga cgcgctggcg cagttgggaa aatcgtcggg
     2941 aacccagagc ctgtaattgc cgccggcgga gccgttgagc agttgcgggc cgggggtggc
     3001 gtacttgtag gtgtggccgg gattgaacgc atcctgcatg ttggcggcca ggccgggctt
     3061 gctgccgcgg gcgaggtatc cttcctgggc cagttcgacc ggaaactggc accagtgggc
     3121 ggacagatca ctgttgcagt cgacgcgcac gggcggcaac tggccgccgt ggtcgtcaga
     3181 gtacatctcg agcgcgaggc cgacgccgta gagttcagcg tggactttga tgaccttggc
     3241 cttctccttg gcgcgcgcca gcgcgggcaa cagcaggccg gcgaggctgg caatgacggc
     3301 catcaccacc agcaattcga ccagtgtgaa tcccgcggat cgttggttcg ccgcgcgcat
     3361 gagaggcgtc agttcgtctt cctcagccgg tacacctggt gcggatcggg ggcgggcggc
     3421 acaagaaccg tgttccggcc ctcgatctcc gccggcctgt tcgtcaccgc cgaccactcg
     3481 gtcgaattca cgtcagcgcg agattcgagc tgataggccg cgccgctgac gggccatgag
     3541 agggcgacgg tgttggcgat gccgagcacc ggcggctctt gcgggattac ctgctgcacg
     3601 aacagctcgt acccattggt gccctggctg ccggaaccgg attcctggtt gaagatgccg
     3661 acggcatcga accggccggc cggggccgcg ccaaggctga agcgcggatg gcgcagccag
     3721 aagaaccgtc cttcgttgtc ggtgacagtg cagcgccgca tcgcccaact gttcgtgggg
     3781 ttccatccgt ttgtcgtcac gagcgtgagg ttgttcaggc gcacccgcat gccttgataa
     3841 tgctcgcccc ccgtggcgcg agtcgggtca aaaatggccg gccacgatgc aggatcgtcc
     3901 gcgctgccgc ccggcagcat gacatcggcg agggtgatca cttcgggcgc tggaaggccg
     3961 tagttgggcg tcacgagccg gatgttgaaa tcaaaggcgg ggtcgatatc gtgtccttcg
     4021 ttgatgttgc gtttaccgcc gtaaaacagc gcctggcggg ccgttacctc gatcaggtcg
     4081 ccggcgcggt actggcgcag gctcgaggga tcgaaactga gccgcaggac ctcccgcacc
     4141 caggcctcgt tgtcgtagct cagatcgctc tcgtgaatcc agggctggtt gccgtagttc
     4201 tgccccatcc agcacatcgt cccgccccgg tcgcccgggt ccacggcctg gaaagccacc
     4261 tgccattcgc cacccatccg atactggttg gccccgttgg cccacggcag gaattgcggc
     4321 gtggcatcga gcatctcgtc cggattgcac agcagcactc cccgcagcgt gaacgggaat
     4381 gacccgttcc acgtgcttgc ccccacgctg ttgaccgcct ggagggcggc gtgcgtctca
     4441 gtctgcgcca gagccggcgc cgcgaccgcg cagaaagcga ttgccattgc aatctggcgc
     4501 gcggcggatt caaatgacat gaccattttc gatttcattc tcgttgtccc taagcccccg
     4561 ctgccccgcg gcgcgcgcgc aggggaggct tgactggccg cgcgcgccag tattactgtc
     4621 gatgcgaagc atgttaccag gaatgccgtg ttacgcaacc caaaaagaat agtatgaacc
     4681 gcgcccacgg atccacgccg gcccgcgacc cgcgcatcgc ctacttcgac cgccaggccc
     4741 cgacctggga ccggacaggc cccgatccgg acgcgacgat gcgccggctt cgggacctcg
     4801 atgggcgtct ggggttgaag gcgggcctcg atctcctcga ggtgggttgc ggcacgggac
     4861 agatcaccgg ctggctggcc gacacggtga ggccgggccg ggttgtggcg gtcgatttct
     4921 cggaggcgat gctggcgcag gcgcgagcgc gcggcgtgga cgccaagttt gtgcttctgg
     4981 acatctgcgg tgaggaaccg ttcgaggggt ctttcgaccg ggtgttgtgt ttccatgcgt
     5041 ttccccattt ccgggaccag gcggcggcgc tgcggcggat ggcccggcat ctcaagcccg
     5101 gcggccagct tctcgtgctg cacctctccg gcagccgcca gctcaacgcc ttccaccaaa
     5161 gcctgggcgg agcggtcggc cacgaccacc tcccgccggc ggcgcgctgg cctgccctgc
     5221 tgcggccagt tgggctcgag gtcagagagg cggtggatcg cgcggacctg ttcctcctga
     5281 aggccgaggc gggcgcgcgc ggcgggactg cgcgacagga ttcatgcatt ccacattgac
     5341 gaccatgaaa agcaagctct gtctcctcgg cggcctgatg cttctggcga ttgttccgct
     5401 gcgagccgcg gactccgttc ggccgaacat tcttttcatc ttttcggatg accatgccga
     5461 gcatgcgatc tcggcgtacg gatcgaaggt caatcagacg ccgcacatcg atcggcttgc
     5521 cagggacggc gtgcgcttcc tcaattcctt tgtcgtcaac tcgatctgca caccgagtcg
     5581 cgcgacgctg ctcacggggc aatactcgca tgtgaacggc gtgccggtgt tcaaccggtt
     5641 cgacggcgcg cgcgatcatg tcgccaagcg gctgcaagcc ggcggctacc acaccgggat
     5701 gatcggcaag tggcatctgg gctccgatcc gaccggcttc gatcgttgga ttgtcctgcc
     5761 cggccaaggg gcgtattgga atccggcgtt cctggtgtcc ggacgccagc tcactattca
     5821 gggtcattgc acggacatca ccacggacct gggcatcgag tggttgaaga cgcgtccgca
     5881 ggacaaaccg ttctttctga tgctgcatca gaaggccccg caccgggcgt gggagcccga
     5941 tgaacggaac aaggcgcttt ttcagaacaa ggtcataccg gagcccgaca cgctctggga
     6001 cgactacgcc acgcgccccg cggcgcttcc ccggaaccgt cagacaatcg cccgggacct
     6061 gacccgccgc gatctcaagc tcgagccgcc gtccgatctc aaaggccccg cccgcaacca
     6121 atggctgcag gaggtgccga tggaactgtc ggtggacggc aagacgctga ccggcaagga
     6181 ccttgtccgc tggaagtacc agcgctacat gcaggattat ctggcgtgcg tgcagggcgt
     6241 ggacgacggc gtcgggagaa tcctggacta cctggaccag gtcggcctgg ctgcgaacac
     6301 cgttgtcatc tactcggcgg acaacgggtg gtatcttggc gatctggggc tctacgacaa
     6361 gcgcttcatg tacgagcccg gcctgcgcgt cccgttactg gcgcgcggcc ccggacttca
     6421 ggccggccac accccctcgc agttggtcgc caacattgac cttgcgccga cgtttctcga
     6481 cctggcaggc ctgcccgttc ccgagtccat gcagggccga tccctagttc cgctgctgcg
     6541 cggcgcggcg ccgtccgact ggcggtcgag tgtctactac cgctactacc acgaccccgg
     6601 ccatcacaac acggccgctc atctcggcgt ccgcacagcc acgcacaagc tcatctacta
     6661 ctggaaacag gacgcctacg agatgttcga tctggtcaag gaccctgccg agcaacacaa
     6721 cctgctgcac tcgccggtgg aggcccagcg ctccgaagtc gcggccaggt ttgccgaatt
     6781 gaaagcggag ctggtccgct tgcagaagga gttcaaggac gacggtcgct acgccgatcc
     6841 cgccacgtgg ccatccggca gttcggacgg tccgtttggc gacaagcagc ccgtgggaat
     6901 caagaccgtc gccgaggcta tcgcgctcac gagcgccgag tagcccggac gaatccgaag
     6961 ggccgaggtt tggttgcggc tttgccgcgc caagatcgat ccgggttagc gggagcggac
     7021 ggtgttcgaa gcaggccggt ttcggagcgc agccagccgt tgttcgagtg tctgcggcga
     7081 cacggttccg gcggtgccca actcctgggc aaacacggag accttgtatt cctcgatcag
     7141 ccagcgcaat gcatcgcgct cgccgcgcac ggcgtcggat gcatcgggcg caatccgaat
     7201 ggcagcgagg gcagcgatcc agggctgcac acgatcgagc ttctcgcggt ctttggctgg
     7261 attcaccgcc gcgcgctcgg cgcgaatcag catcgccttg agatagcgcg caaggtgggg
     7321 gagtcgatcg aacgggactt gcccgagaaa gcccgcaggc acgaggcggt ccagatcctc
     7381 ccgcattcgc ggataaggct ttcggcagag aaggatggcc tggcgcaggc gcagaatctc
     7441 ggccaatcga tccgagagtt gaggcgccag gccgcgaata gcggcgcggg tcgactcgcg
     7501 aagcgccgcg aaggccgcgg ccgtccgggg cgaggcggcg gacgcagtca acagatggcg
     7561 tcgcaagttc gcgaaagccg tggcttcgag ttcatcgaca ggcccgatcg tgatgtaaag
     7621 gaggcgccat tggttcagac cgcgcaggtc ctttgcgagc cacgcgagtt cccgttgcag
     7681 ccccatttcc agcagacgcc gcacgcctcc cgcgtgcgcc tcaatcgcag ccgcggcagt
     7741 gcgaaacagc cgcagcgaga cctcgcccgc ctcgacgtgc agggcgggga aggcctcgat
     7801 tggaaacccc gcgacatccg caacctgaat gcggtcgggc aagtctccgc aggtccagtc
     7861 cttcagtccg taacgctccc agcgcgccac ggcgctccgc cacgcctcgg actcgatggt
     7921 ggtgtcgtgc gattcgagtt caacctgcaa cgcgcgcaga tcgcgtccgc atgccaatgg
     7981 ctggttatcg cgtcccagaa tctcgaagcg cggacgcagg tgggccggca gcaactcgac
     8041 gctccaggcg gtgctgggca cctcgacgcc gtagcgctcc cggatgaatc ggatcagggc
     8101 cgggagaaac gcgcttccgt cgggcttgat ggcctgggcc atttccttcg cccggggcgc
     8161 gagcggcatc aacgggcggc gcaaatcctt gggcaacgcc tgcagaagat ggagaatctg
     8221 aggctcgcgc aaacccggca ccgcccaatc gagcaaatgc ggctcgatca cctgcgccag
     8281 cgtaaagggc aaccggaccg tcacgccgtc gcgatcgtcg cctggcgcat agtcgtaggc
     8341 gacagggacc tgctgggtgc caatggcgac ggcatccgga aacgcgagtc cgtcgaatgc
     8401 gccggcgtgt ttcccgagca ggtccgccgc atcgagacac agccaggacg ttccctcggc
     8461 ccggcgctcg cgcagcagat ggttgaggtc ggccaccgac cccacattcg acagccgttt
     8521 cgcgtatgcc cggtgcagcg cttcgtccag atcgggcacc agccggtgct gcaatcggct
     8581 ctgccaaagc tcgatcttct gccgcagttg atggttgtgc cgcaggaact gataggccga
     8641 tggcagcgac gcgaggtctg gcggcggcgg atcgacgcgg gattgcccga gcgcggcttg
     8701 ctcgcggcgg ccctgaccag cgcccccggc ccgcgcagtc agcgattgcg ccgcgttgtc
     8761 ctggactcga gtggcgctcc gaaagtgatc ggccagacct tccgagatca gcgccgcgcg
     8821 gatgaagatt tccgtcgcct ccgccggatg gctttgccgg taggacacca tccgctccga
     8881 gaccaccagg ccgttgagcg tcacccgttc gagcgccgca acgcgcccgg cggtggggtc
     8941 ccagtgcggg tccaaatggg ctttgcggat caggtgcggc gccagatcga gaatccaccg
     9001 cggctcgatt tccgccaccg tccgaacgaa gcggcgcgag gtctcgacga tttcgccggc
     9061 gacgatccag gccggctgcg tttgagaccg gggcggggct ttcggcgcgt ccttgtgccc
     9121 cgattcgaca cgcctttcag tccccctttc gaacagacct gacccgggga aaaccatggc
     9181 ttcgcgattc ccggcaagcc gatacaggtt ccgttccttc ctggccgcca cgtggctgaa
     9241 caggccggtc agaatcgacc gatgcacggc ggcgtaccag gaatctgctc cgcggccggc
     9301 ggtctgttgc tgttcggact gcgcaatgaa ccccgcagcc ggcgtggtga gatggcgctg
     9361 attcctgcct ttgacggagc gagatggcgc ggactcatcc cgcaccgcgg ggcggggaca
     9421 cggttcattg gaagcaagcg tgtgcggtcc ctgagtcagc gcatcttcga gggtttcgtc
     9481 cgcatcgtcc ggcgcggcgg ggcctggcgg cccgcccatg tgcgcgacgg tctcgcggag
     9541 ttgggcgtgg atgtcgcgcc actcgcgcat ccgtgggaac gacaagaaat gctcgcggca
     9601 gaacttgcgc atctggctct gcgtcttgag cgattcaagc gactcgtggc aggcctccca
     9661 aatccgcacc agcgtcagga aatccgaccg cgggtgttga aagcggcgat gggccgcgga
     9721 cgctgcgtcc tggcggtcgg cggggcgttc gcgggggtcc tggatgctca ggccggccga
     9781 gatgatcagc accggttcga ggacgccctc gatctgtgcc tggaggatca ttcggccaat
     9841 ggtcgggtcg acgggcaggc gcgccagccg ctcgcccagc ggcgtcagtc gccggtcggc
     9901 atcgagcgcg ccgatctcgg ccagcaactg gtacgcgccc gcgatcgccg ccggcaaggg
     9961 cgggtggatg aaaggaaact cctcgacctc gcccagatcg aacgccttca tccgcaggat
    10021 gacatcggcg agattcgccc gctggatttc gggctgtgtg taaagcgggc gggcctggaa
    10081 ctcctcctcg ctgtaaagcc gataacaaac cccttcggca acgcgcccgc agcggccctt
    10141 gcgctggttg gcgctgctct gcgatatggg ctcgattggc aaacgccgcg ttcgggtccg
    10201 ggcattgtaa cggctgatcc gcccaaggcc cgtgtccacg acataacgaa tgcctggcac
    10261 cgtcagcgac gtctcggcga tattggtcgc gatgacgatc ttgcgccgct ccgacgcggc
    10321 gaacacgcgc tgctgctcgt cggtgctgag ccgcccaaac agcgggacaa gttcgagtcg
    10381 tccgccaccc gcctcccgca gggcgtcgcg cgtctcaatg atgtcacgct cgcccggcat
    10441 gaacacgagc acgtcgcccg aggccgaagt ggcgaggatt tcctggacgg catccaccgc
    10501 ggcatcgaca tacgtccggc cgtcctccgt ccggtcgccg tcttcatcgc ccgagccggc
    10561 gccgaatggg cggtattcca cagtgacagg gaatagacgc cccgagacgg tgatgatggg
    10621 cgcgtcatcg aagtgccgcg caaaagcctc ggtgtcgatc gtcgcggacg tgacgagcag
    10681 cttgagatcg cttcgctggg agagcagacg cttgagcaag ccgatcagga aatcgatgtt
    10741 cagggaccgc tcgtgcgctt cgtccagaat caccacctcg tactcgctca gcaccggatc
    10801 ggcctgcgcc tcggcaagca gaatgccgtc ggtcatgaac ttgacgtacg tctccggact
    10861 ggtctgatcc gagaaccgga ttttgcatcc cacggcgcgg ccccacgggc ggtcgatttc
    10921 gtcggccacg cggcgtgaaa gcgagagcgc cgcaacacgg cgcggctgcg tgcacccgat
    10981 cttggcccgc acgccgaaac cggcctccaa acaaatcttc ggcaattgcg tggtcttgcc
    11041 cgacccggtc tcgcccgcga cgatcaacac ctggtgcgcc cgcaaggcat cgagaatctc
    11101 gttgcgtttg gcactgatgg gcaactcgtg cgggtagcgc agctcgggca gggccgcctg
    11161 tcgacgcgca cggcgggcga tcgagtctcg cgcctcgtcg agccaccgtg gcagatcgtg
    11221 cggcgcgcgc gagctctgcc ggtgggcttc gagccagcgt gtgaccttcc agccaatccg
    11281 cagttgatcg gtcagcagac accgcggcag aagccgttcg agttccttga gcgaggcgac
    11341 gggattcacg gaagcagcgg cacgcatcgt tcggttgaaa aatgaggttg aaccgctcag
    11401 ggcgaacaca tgccgttcac gatctccaac tcacttgccg agcaggcctc tcatacgcgc
    11461 caagacctct atcgccagga agagccggtg tcaggcgcaa agttgcggcg gggatgccgc
    11521 agctcaggtt tggcacctca cccggaactc atcactcccg gcctgctgac gctcatgctc
    11581 aagagcacgc taggatactt agcatttggt catcctttta gtataaatcc gcgtctgcac
    11641 cccttcagaa ctcaacgctg agcccgcatg tttcatggag tcgtgaccat cgcgaagccg
    11701 aacactccag gacgtggacc catgcgggtg caaactcctg tggaggtact ggttaacccc
    11761 tcagtggggg aatttcatcg acgacgcccg cgggcgatgc tatggactga cccgccattc
    11821 actggaacat gaaagcctgc caagtcgcga tgagccttgc cgtggcgacg gggtggctcg
    11881 ctgtgtcgtc tcgagccgcc gagacggagc tgcgccccgt ggtcgaggtc gaggaggtga
    11941 tttacgaata cacgcccgcg gacaacgggg cggggccgat gtggtgtcac gggtccacgt
    12001 gcctggtgcg gttgggcgac gccgtgttcg caagcggact cgagaccatc ccgcacgaga
    12061 aaccgctcaa caattgtcga tggactttct ggagacgcgg accggaaggc tggacccttc
    12121 tccgacagga ttcggttggc cgcacccgcg agccgtgtcc gctggcggca tttcccgaac
    12181 cgggccgggt ctttctctcg gcgaatccga ccctcaatcc gcctggacag gctggcgggg
    12241 ggccggcacg cccggtgctt ttcgagttca tggcagcgga tgcgtccgcc ccgcccggga
    12301 gccattggcc gggctgggag tgcaagccgg cgcctccggc gttcaccgag cattcctacc
    12361 gcagtctggc cgcggacgga ccgggccggg aaatgattct cttccagaac atcggctaca
    12421 cgcatgccga gtgggctttt cgggatcgcg acggcaactg gagcgcgcag ggtcggctca
    12481 aatggccctg gggcgccgag tacgacactc cccagcccat ccgtgtctgc tatccgaacg
    12541 tggcgctccg cgcgcgcgcc gtccatttcg tcggagtcag tgacatcgtc gagccgtacg
    12601 cgaagtggcg tcaggccaag cgcgaactca ctggccagga ctgggattac gattttcgcc
    12661 gcctcttcta tgtgtggtcc cccgacatcg cccacggcgg ctttggcgac tgggtcgaaa
    12721 tcgccagccg cgacaagaca tgcgggtgga tttctccggg cgatctctgg gttgcccccg
    12781 acgacgccgt ccacattgtc tggacggagc gcgcgttgga cgaacggctg cgcccgaagt
    12841 tcttcccgaa cgagaggcag cggcacgaac tcaactatgc gcggctgcgc gaaggccaga
    12901 tcgagacgcg gcgcacgctg ctcgccgtcg
//
LOCUS       DAOQRO010000049        12920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_3933_length_12920_cov_4.778313, whole genome shotgun sequence.
ACCESSION   DAOQRO010000049 DAOQRO010000000
VERSION     DAOQRO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_12920_cov_4.778313"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PKK20_03155"
     CDS             <1..1153
                     /locus_tag="PKK20_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU98917.1"
                     /translation="RSRGHGLGGGNDEREQTLNQLLVEMDGFDTTDGIIIIAATNRPD
                     VLDPALLRPGRFDRQITVNLPDVRGREAILKVHAKNVKLAPNVDLSVIARGTPGFSGA
                     ELANLLNEAALLAARFNKKAITMAELEEARDKVRWGRERRSLAMTEEQKRFTAWHEAG
                     HALVNVLLEHTHPLHKVTIIPRGQALGATMYLPKDDILNRGRKELLDLITVTMGGRIA
                     EEIISGDFSTGAAGDIQQATLMARSMVCSFGMSEKLGMVQYGDDSEYIFLGRDMVRSK
                     DYSEETARDIDAEVKRITNECYQRAREIIYGNRDKLELIATSLLEYETLEGAQVEEIV
                     RTGRLTPPPPAPQTGPPTGAQAATPLSEIAKPVPPKLPPGLTAPAPATA"
     gene            1164..2093
                     /locus_tag="PKK20_03160"
     CDS             1164..2093
                     /locus_tag="PKK20_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNU98918.1"
                     /translation="MNPLRSWRLGLLGLWLGAFAALAQDYQLFARTNLVAWCIVPFDA
                     LKRGPTERAAMLDRLGLRLFAYDYRAEHIPTFDAEMEALKQRNVRLLAWWFPTVLNDE
                     ARLILEVLRRHDIKAQLWVSGGGEAVSGAEAARARVEDEARRVRSIAEAAGRIGCTVA
                     LYNHGGWFGDPDNQIAIIERLRAQGVTNVGIVYNLHHGHEHLDRFPALLDRMKPHLLA
                     LNLNGMTRGGDAVGKKILPLGQGDLDLSLLKTIRDSGWRGPVGILNHTEEDAEERLAD
                     NLAGLEWLVKQLDGGVPGPRPIPFRESRGRKKG"
     gene            complement(2090..3334)
                     /locus_tag="PKK20_03165"
     CDS             complement(2090..3334)
                     /locus_tag="PKK20_03165"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNU98919.1"
                     /translation="MLIDLRQQFPPSKTLDLDRVAQEATAAVVQHLKPGARVAVAVGS
                     RGIANLQTIVRAAVRALEAAGARPFILPAMGSHGGGTSEGQVALLADYGITESAVGVP
                     IRPSMEVQIVGSVIPGFDLCVSREALSADGVLLINRIKPHTDFAGSIGSGLLKMLVVG
                     LGKQAGAAQFHAAACRLGYEHVLRAHSRALLETVRIIGGLAILEDQRHQTARIDAVRP
                     SDIEAREPLLCDEASAMMPGLPFDDIDLLIVDRMGKNLSGTGMDPRVIGRSIHGYSLA
                     EETPRSGPRIRRLFVRDLTPESHGNAIGIGLADFTTTRLVQAMDRGLTTVNSLTALSL
                     QGAKIPLHFQTDREVLEKAAATLGLANMQNARIVRILDTLHLEHLEVSVPLAREIEGR
                     PGLETSGDPHPFEFDAAGNLAG"
     gene            complement(3358..5061)
                     /locus_tag="PKK20_03170"
     CDS             complement(3358..5061)
                     /locus_tag="PKK20_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HNU98920.1"
                     /translation="MTLSARTLTGLLAGFAIRLWAGGDEVVVVYNTEFRPDSRLVAEH
                     YAEKRAVPDRQVIGLSLPRAEGMTRTEFEKRLQEPLLAALEQRGLLTFYDQIVPATGN
                     LPGRVVRLPVAATVRYVALCYGVPVQILEDAKPTGTNKTLLPAQRRAAAVDSELALSP
                     ALYQRLPIEGVLRNPFMGTEALSRLDPKGGLLMVTRLDGPSGSVARNLVDNAIAAETH
                     GLWGRAYFDARGLTKGGYKVGDDWIIAASAAARRHGFETVLDRDPATFGPGYPMPQTA
                     LYAGWYDQAPSGPFAQSTVEFMPGAIAYHIFSYSARSIRTADTWVGCLLDRGATATVG
                     FVTEPYLESTIDVGIWFSRLIESGATFGEATYAALPSLSWQTTVIGDPLYRPFPIRTD
                     SSPARPPALPESLVPWSRLREINLRLVRGDTPTRVLEELARAPETRSSALLEQKLGDL
                     CEQAGKLDDAVEAYRRALALRPSDQQRVHLQLGVARLLVALRQSDAAISAYEQFLKQN
                     PKYPDRIGIYRKALLLAREINSESLIRRYEAEIQYLTPPPPATNAPPPASKAPVPAKS
                     K"
     gene            complement(5058..5840)
                     /locus_tag="PKK20_03175"
     CDS             complement(5058..5840)
                     /locus_tag="PKK20_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU98921.1"
                     /translation="MSFRLTFLGTGTSQGVPMIGRDYPPAFLANPRNHRLRPSVYVET
                     DAVRFVIDTTPEFRLQMLRERIGWLDAVLFTHGHADHIMGLDDCRRICDVRPGPLPVY
                     ASAATLADLKRVFYYAFDGRPIPKGYFNPDPHVIEGPFRIGDLDVLPFNLPHGRFVTT
                     GFVFLQGNSKRLAYFSDCKEVPAPAAEAARGVEVAVLDALRRTPHPTHMNLDEALTAA
                     RRIGAGRTFFTHLTHEFDHDTDQAELPKGVAFAYDGLKLELK"
     gene            5901..7583
                     /gene="recN"
                     /locus_tag="PKK20_03180"
     CDS             5901..7583
                     /gene="recN"
                     /locus_tag="PKK20_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNU98922.1"
                     /translation="MLTTLRIRNLALVSEVTLELPSGLVAITGETGAGKSILLGALGL
                     VLGERADRSLIRSGQESCAVEAVFDVTLMSRAFQRSLDDQGLDSCADGQLILKRTFTA
                     SGANRQFVNGTPTTLQTLSRVGDWLVDLHGPHEHQSLLDPSRQLALLDAFGVLDARRA
                     SVEALVRERDALETQKADLIIDDRAYAQQLDLLRHQTAEIAAARLSPDEEETVAREYQ
                     RASSAAQRLQFAQAAADALGGEEGSVLSQAGAVGRLLQELDRTDSDARPLLDAFEQGI
                     ASLRDLERDLSRYAERIDLDPARLQGLEERLNLIQTLKRKYGNSIDAVMRFGEEAARR
                     LEQLEGRSEELARLELALARVSSELWDAALDLSARRRECGARLARAVSGQLADLGFRR
                     SEFDVRIVSLARNPPPHPQPGSAGIDTIEFHFAPNPGEPAKPLRSIASSGELARAMLA
                     IKTVLAVQDQIPVLVFDEVDANVGGETANAVGEKMRTIARHRQVLCITHLAQVAAVAD
                     AHFAVTKEIRGGRTLSTIHRLDHSGRVTELARMLGGHTEAARRHALSLLRQP"
     gene            complement(7586..8215)
                     /locus_tag="PKK20_03185"
     CDS             complement(7586..8215)
                     /locus_tag="PKK20_03185"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNU98923.1"
                     /translation="MPHQLPPLPYAKEALEPHIDAQTMEIHHGKHHQAYVTNLNKAIA
                     GNAALEAKTIEALIKDLAAVPDAVRGAVRNNGGGHWNHTFFWNIMAPNAGGAPSGAVA
                     AAINAAFDNFDSFKEKFEAAGLGRFGSGWVWLIAKAGKVEIASTANQDNPLMGSSIAG
                     CDGTPILGVDVWEHAYYLKYQNRRADYLKAWWNVVNWAAVAKQYEAAAR"
     gene            8614..9939
                     /locus_tag="PKK20_03190"
     CDS             8614..9939
                     /locus_tag="PKK20_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU98924.1"
                     /translation="MLCRVSALVLAGLLLCSCGKPAAGSKARQNGAEPARPVRLAAVE
                     VRLMERSIKATGTLAAYEESTLSAKVPGRLQQLMVDIGSVVRAGDLLAQVEPRDYELR
                     LQQAGAALAQARVALGLPLDGDDDEIALDEVSSVKQAKAVLDEASKNQDRVARLLKSG
                     IASDAEFDTVEATHTVAAAKYATALEEARMRMATLSQRRAEYEIARKQLADASVRTPF
                     DGSVQSRPASVGEYVATGAPIVTVVRTDPLRLRLVVPERESMLVRAGQTVRLRVEGDA
                     TTYPGTITRLSPALVEEDRMLRVEADVPNPGSLRPGMFASAVIVVDDRQEALTVPTNA
                     VVTFAGIEKVVLVQDDKALEKDIATGRQRMDRIEVLSGLTLGQTVVLDPAGLRTGQRV
                     TVESTLSETPSAPAVGGQSTATGAGGDAGAEAARSELGAANRQSNAEAR"
     gene            9923..>12920
                     /locus_tag="PKK20_03195"
     CDS             9923..>12920
                     /locus_tag="PKK20_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU98925.1"
                     /translation="MQKLAEICIRRPVFATMIVMALVVVGVAGYVNLGVDRFPSVDLP
                     TVRIMTRLPGASPEEMESLVSQPIEEVVNTIEGIQELRSVSGNGSSFVIVTFELNRDI
                     DVAAQDVRDRVSTVLRHLPDDTDPPTIAKTDADQAPIMTLAVSGPRSRRELTEIADKI
                     IKTQIERSLGVGEVNVYGGLERAINIWVDADRLAAYRIPITAVREALVRQNANIPGGN
                     VTGPLREHTLRTIGRLNDPRAFNDLVIATRNGSPIRIRDIGMAEDGTKEQRSVSRLDG
                     VPTVSMEIVRQSGANTVAVIEAVKANLEVLRAQLPSDVRVEVIRDQSRYIYEALHEIR
                     QHLILGSILACLVVLLFMRNGRAMVIAAVAIPTSVIATFGMMKALDFTLNSVTMLALV
                     LMVGIVIDDAIVVLENIFRFVEEKRMRPFEAARAATAEIGLAVMATTFSLVVIFVPVS
                     FMSSISGRFLYQFGITAAVAVLVSLLVSFTLTPMMSARLLHVNGGGDQGAAPRSRSGF
                     YHWIDQVYTAALAWAVGRRWLVAALALAVIVTSVPLYQAVRQEYLPSNVDEGEFDVRV
                     TTAEGTGLVSMNEVSERIEKELLGTPGVDLVLSSTGSGFMGSVNNAGFFVRLKPHAER
                     TFRWTRLFQWPPWRAFENNYTQRDIQRMIRARLAGIPSIRFQVRNPQTFVGGGPNFDI
                     DFALLGPDLTTLWRYAEALREKAPELGLLDADTTLKLDTPELRVEIDRDRAANLGVDT
                     QDIATALRIMVGGDTEVTRFRDESMNEDYDVAIRLKEGDRNDPATIASLFVPRQGGLV
                     RLDNVVKLVPTQSPSRIDRLDRQRQVSLRASVAPGFAQADRIAALRAEVAKMGLPTGY
                     STRVSGRARELETTFNEFLWAFALSVVLMYIILASQFESLVHPFTILLSLPLSVPFAF
                     LSLLIAGQTINLYSALGLLVLFGVVKKNAILQIDHMNKLRAAGLDRAAAILRGNRARL
                     RPILMTTMTLIAGMTPLALGTGPGAAE"
BASE COUNT         2221 a         3991 c         4399 g         2309 t
ORIGIN      
        1 ccggagccgt gggcacgggc tgggcggcgg gaacgacgag cgggagcaga cgctcaacca
       61 gctcctggtc gagatggacg ggttcgacac caccgacggc atcatcatca tcgccgccac
      121 caatcgcccg gacgttctcg atccggcgct gctgcgtcct gggcgtttcg accggcagat
      181 cacggtgaat ctgccggatg tgcgcgggcg cgaggccatt ctgaaagtgc acgcgaagaa
      241 cgtgaagctg gcgccgaacg tggacctgtc ggtgattgcg cgcgggacgc cgggattctc
      301 gggcgctgag ctggcgaatc tgttgaacga ggcggccctg ctggcggcgc gtttcaacaa
      361 gaaggcgatc accatggccg agttggagga ggcgcgcgac aaggtgcgct gggggcgcga
      421 gcgtcgcagc ctggcgatga ccgaggagca gaagcggttt accgcgtggc acgaggccgg
      481 gcacgccctt gtgaacgttc tgctcgaaca cactcacccc ctgcacaagg tgaccattat
      541 tccgcgcggc caggcgctcg gcgcgacgat gtacctcccc aaagacgaca tcctcaaccg
      601 gggccgcaag gagctgctcg atttgatcac cgtcacgatg ggcgggcgca tcgccgagga
      661 gatcatctcg ggcgatttct cgaccggggc ggcgggcgac atccagcaag ccaccctaat
      721 ggcacggtcg atggtctgca gtttcggcat gagcgagaag ctggggatgg tccagtacgg
      781 cgacgacagc gagtacatct tcctcggacg cgacatggtg cggagcaagg attacagcga
      841 ggagacggca cgcgacatcg atgcggaagt gaaacggatc accaacgagt gctaccagcg
      901 ggctcgcgag atcatttacg gcaaccgcga caagctcgaa ttgatcgcca cttcgctgct
      961 tgaatacgaa acgctcgaag gcgcccaggt cgaggaaatc gtccgcacgg ggcggctcac
     1021 cccgccccct cccgcgcctc agaccggacc tcccaccggc gcgcaggcgg caaccccgct
     1081 gtcggagatc gccaaaccgg tcccgcccaa gcttccgccc ggcctgaccg cgccggcgcc
     1141 cgcgaccgct tgagattgag ccaatgaatc cgctccgaag ctggcgcctg ggtctcctgg
     1201 ggctctggtt gggcgccttc gcagccttgg cccaggatta ccaactcttc gcccgcacga
     1261 atcttgtcgc atggtgcatt gtccctttcg acgctctcaa gcggggcccg accgagcgcg
     1321 cggcgatgct cgatcggttg ggactgcgtc tgttcgccta cgactatcgg gccgagcaca
     1381 ttccgacctt cgatgccgag atggaggctc tcaagcagcg caatgtccga cttctcgcct
     1441 ggtggttccc aacggtcctg aacgacgagg cgcggctcat cctcgaggtc ctgcgtcgcc
     1501 acgacatcaa ggcccagctc tgggtgagtg gcggcgggga agcggtgagc ggggcggagg
     1561 ccgcgcgtgc ccgagtcgag gacgaggcca ggcgggtgcg ttcgatcgcg gaggccgccg
     1621 ggcggattgg gtgcacggtg gcgctctaca atcatggcgg ctggttcggc gatccggaca
     1681 accagatcgc catcatcgag cgcctgcgcg cccagggcgt cacaaacgtc gggatcgtgt
     1741 acaatcttca ccacggccac gagcacctcg accgttttcc cgccctgctc gaccggatga
     1801 aaccgcatct gctggctctc aacctcaacg gcatgacgcg cggcggcgac gccgtcggaa
     1861 agaaaatcct tccgctcggc caaggcgacc tggatctctc gcttctcaag acgatccgcg
     1921 acagcggttg gcgcgggccg gtcggcatcc tgaaccacac ggaagaagac gccgaggaac
     1981 gattggccga caacctggct ggattggagt ggctggtgaa gcagttggac gggggtgtgc
     2041 cgggaccaag acccatcccg ttccgtgaga gccgcggaag gaagaagggc taacccgcca
     2101 gattccctgc ggcgtcgaac tcgaatggat gcgggtctcc ggacgtttcg agacctggcc
     2161 gaccctcgat ttcgcgggcg agcgggactg agacttccag atgctcgagg tggagagtgt
     2221 cgaggatgcg cacgatgcgg gcgttctgca tatttgccaa cccgagcgtt gcggcggcct
     2281 tttcaaggac ctcgcggtcg gtctggaagt ggagggggat tttggctcct tgcagcgaca
     2341 gtgccgtcag agaattcacc gtggtgagcc cgcgatccat ggcctgaacc aggcgcgtgg
     2401 tggtgaagtc cgccaacccg attccgatgg cattgccatg gctttcgggc gtcaagtcgc
     2461 gcacgaagag gcgccggatg cggggcccgg atcggggtgt ctcttcggcc agcgaatacc
     2521 cgtggatcga gcgtccgatg acacgcggat ccatgccggt tccgctcagg ttcttcccca
     2581 tgcgatccac aatcaggaga tcgatgtcgt cgaatggcaa cccgggcatc atggcgctgg
     2641 cctcgtcgca gagcaggggt tctcgcgctt caatgtccga ggggcggacc gcgtcaatgc
     2701 gggccgtttg gtgtcgctgg tcttcaagga tggccagacc gccgatgatg cgaacggtct
     2761 cgagcagtgc gcggctgtgg gcgcggagga cgtgctcgta tcccagcctg cacgcggctg
     2821 cgtggaactg ggccgctcca gcctgtttgc ccaaacccac caccagcatc ttgagcagac
     2881 cgctcccgat cgatccggcg aagtcggtgt gaggtttgat ccggttgatc aggagcacgc
     2941 cgtctgccga cagcgcctcg cggctgacgc acagatcgaa accgggaatc acgctgccga
     3001 caatctggac ctccatcgaa gggcgaatgg gaacacccac agccgattcc gtaatcccgt
     3061 aatccgccag cagagcgacc tgtccttcgg acgtgccgcc gccatggctg cccatcgccg
     3121 ggaggatgaa cgggcgggcg ccggccgctt cgagcgcgcg caccgccgcg cgcacgattg
     3181 tctggaggtt ggcgatgcca cggctgccga ccgcgacggc cacgcgcgcg ccgggtttga
     3241 ggtgctgaac aactgcggca gtggcctcct gggcgacccg gtccaggtcg agtgttttcg
     3301 agggcgggaa ttgttggcgg aggtcgatca acaagggcca caggggtaag cgcgagttca
     3361 tttcgatttg gctgggaccg gggctttcga cgccggcggc ggggcgttgg tggcgggggg
     3421 cggcggagtg aggtactgga tttcggcctc gtaacgccgg atgagcgatt cgctattgat
     3481 ctcgcgggcg aggagcaggg ccttgcgata gatgccgatc cgatcggggt acttcggatt
     3541 ctgcttgagg aactgctcgt aagccgaaat ggcggcgtcc gattgccgca gggcgacgag
     3601 gagtcgggcg acaccgagct gaaggtgaac gcgttgctgg tcgctggggc gcaacgcgag
     3661 ggcgcggcga taggcctcga cggcgtcatc gagcttccct gcctgttcgc acaagtcgcc
     3721 cagcttctgt tcgagcaaag cgctcgatcg agtctccggg gcgcgcgcca actcttcaag
     3781 gactcgggtg ggcgtgtcgc cgcgcaccag gcgcagattg atctcccgca gacgggacca
     3841 cggaacaagc gattcgggca gggcgggcgg gcgcgcagga gagctgtcgg tgcgaatcgg
     3901 gaaaggccga tagaggggat cgccgatcac ggtggtctgc caggacagag agggaagggc
     3961 ggcgtaggta gcctcgccga aggtcgcgcc ggattcgatc agacggctga accagatgcc
     4021 gacgtcgatg gtggattcga gatacggctc ggtgacgaac ccgacggtgg cggtggcgcc
     4081 gcggtcgagc aggcatccga cccatgtgtc ggcggtgcgg atcgagcgcg cgctgtagga
     4141 gaagatgtgg taagcgatgg cgccgggcat gaactcgacc gtggattgcg cgaaggggcc
     4201 cgagggtgcc tggtcgtacc agccggcgta gagcgccgtc tggggcatgg gatagcctgg
     4261 gccgaaggtg gccgggtctc ggtccaaaac cgtctcgaaa ccgtggcggc gagcggctgc
     4321 ggacgccgca atgatccagt catcgcccac cttatagccg cctttggtca ggccgcgcgc
     4381 atcgaaatag gcgcggcccc agaggccatg tgtttcggcg gctatggcat tgtcgaccag
     4441 gttccgggcc acgctgccgc tgggaccgtc cagtcgcgtc accatgagaa ggccgccctt
     4501 tggatcgagt ctggacaacg cctcggttcc catgaacgga ttgcgcagca cgccctcgat
     4561 cggcagtctc tgataaagag cgggcgacaa ggccaactcg ctgtcgacgg ccgcagcccg
     4621 tctctgggcg ggcagcaacg ttttgtttgt gccggtcggc ttcgcgtctt cgagaatctg
     4681 cacaggaact ccgtaacaaa gggcgacgta acggaccgtg gcggcgacgg ggagacggac
     4741 gacgcgccct ggcaggttgc ctgtggcagg cacgatctgg tcgtagaacg tgagcagtcc
     4801 ccgctgctcg agagcggcca gcagcggttc ctgcaggcgc ttctcgaatt cggttcgtgt
     4861 catcccctcg gcccgtggca ggctgagccc gatgacttgc ctgtcgggca ccgcgcgttt
     4921 ctcggcgtag tgctcggcca ccaggcgcga atcgggtcgg aactccgtgt tgtagacgac
     4981 aacgacttca tcgccgcccg cccagagccg gatggcgaag ccggcgagca gacctgtcag
     5041 tgtgcgggct gagagagtca tttcaactcg agtttcaatc cgtcgtatgc gaaggcaacg
     5101 cctttgggca gttcggcctg atcagtgtca tgatcgaact cgtgggtcaa atgggtgaag
     5161 aatgtccgcc cggccccgat ccgtcgggcc gcggtcaggg cttcatcgag gttcatgtga
     5221 gtcggatgag gcgtccgacg gagggcatcg aggacagcga cctcgacacc tcgggccgct
     5281 tcggcggcgg gggcgggcac ttctttacag tcgctgaagt aggccaggcg tttgctgttc
     5341 ccctgcaaga agacgaaccc cgtggtgaca aatcgtccgt gcggcaggtt gaaggggaga
     5401 acatccagat cgccgatccg gaacggacct tcgatcacat gcgggtcggg gttgaagtag
     5461 cctttgggaa ttggccgtcc gtcgaaggcg taatagaaca cccgtttcag atcggcgagg
     5521 gtggcggctg aagcgtaaac cggcaaaggg ccggggcgca catcgcagat ccgacggcaa
     5581 tcgtcgagtc ccatgatgtg gtcggcgtgg ccgtgagtga acaggacggc gtccagccat
     5641 ccgatgcgtt cgcgcagcat ctgcagacgg aactccgggg tggtatcaat gacaaatctg
     5701 acggcgtcgg tctcgacata caccgacggt cgcaagcgat gattccgcgg attggcgaga
     5761 aacgcgggcg gatagtcccg gccgatcatc gggacgccct gcgacgtccc ggtgcccagg
     5821 aaagtcagac gaaacgacat gttttctttt gcctgaacct agcagacccg acacattggg
     5881 cgcgaacgaa aagtcttgag atgctgacca cgctccgcat ccgcaacctg gcattggtgt
     5941 ccgaagtgac cctcgaactc ccgtccgggt tggtggccat caccggcgaa accggcgccg
     6001 gcaagtcgat ccttctgggc gcgctgggtc ttgttttggg cgaacgcgcg gaccgcagcc
     6061 tcattcggag cggccaggag agctgtgccg tcgaggcggt gttcgacgta acgcttatga
     6121 gccgcgcttt ccagcggtcg ctcgacgacc agggcctgga ttcgtgcgcc gacggacaac
     6181 tgattctcaa gaggacgttc accgcctccg gcgccaaccg ccagttcgtc aacgggacgc
     6241 caaccacgct ccagaccctt tcgcgcgtgg gggattggct ggtcgatttg catgggccgc
     6301 acgagcacca gtctctcctc gacccgagcc gccaactcgc cctgctagat gcgttcggcg
     6361 tgctcgacgc gcgccgtgcc tcagtcgaag ctctggtgcg cgaacgggat gccctcgaga
     6421 cgcagaaggc cgatctgatc atcgacgacc gcgcctacgc ccaacagctc gatctcctcc
     6481 gtcatcagac ggccgagatt gccgccgccc gactctctcc cgacgaggag gaaaccgtcg
     6541 cgcgcgaata ccagcgcgcc agcagcgccg cccagcggct tcaatttgca caggccgcgg
     6601 ccgatgccct cggaggcgag gagggctcgg tgctgagcca ggctggcgcg gtcgggcggt
     6661 tgttgcagga actcgatcgc acagattccg atgccaggcc gttgctcgac gccttcgagc
     6721 aggggattgc ctccctgcgc gatctcgaga gggacttgtc ccgttatgcg gagcgcatcg
     6781 atctcgaccc ggctcgtctg cagggtctcg aagaacgtct caacctgatt cagactctca
     6841 agcgcaaata cggcaactcg atcgatgcgg tgatgcgatt tggcgaggag gccgccaggc
     6901 gcctcgaaca actcgaaggg cgctcggaag aactggccag actcgagctc gcattggccc
     6961 gggtgtcgtc cgaactctgg gacgccgcgc tcgacctttc cgcccgccgc cgtgagtgcg
     7021 gcgcccgact cgcccgcgcc gtcagcggac aactggccga cctcggcttt cggcggagcg
     7081 aatttgacgt gcgcatcgtg tcgctcgcac gcaacccgcc gcctcatccc cagcccggct
     7141 cggccggcat cgacaccatc gagttccact tcgcgcccaa ccccggcgag ccggccaaac
     7201 cgctgcgctc cattgcgtcg tccggcgagt tggcccgggc catgttggcg atcaaaaccg
     7261 ttctggccgt tcaagaccag attccggtgc tcgtcttcga cgaggtggac gccaatgtcg
     7321 gcggcgaaac agccaatgcg gtgggtgaaa aaatgcggac gatcgcccgg caccgtcagg
     7381 tgctatgcat cacccatctc gcccaggtcg ccgcggtcgc cgatgcccac ttcgccgtga
     7441 ccaaggagat tcgcgggggg cggaccctgt cgacgatcca tcgcctggat cactcggggc
     7501 gcgtcactga actcgcccga atgctgggcg ggcacaccga agccgcccgg cggcatgcgc
     7561 tgtcgctgct ccggcagcct tgagactacc gcgccgccgc ttcgtactgt ttggccaccg
     7621 cggcccaatt gacgacgttc caccatgcct tcaggtagtc ggcgcggcgg ttttgatact
     7681 tgaggtaata ggcgtgttcc catacatcca cgccgagaat cggtgtgccg tcgcagccgg
     7741 cgatcgagct ccccataagc ggattgtcct ggttcgcggt cgaggcgatc tcgactttgc
     7801 cggccttggc gatgagccag acccacccgc ttccgaaccg gcccaggccc gccgcctcga
     7861 acttctcctt gaaggaatcg aagttgtcga acgcggcgtt gatggcggcc gcgaccgcgc
     7921 cgctgggcgc gcccccggca ttgggcgcca tgatattcca gaagaaggtg tggttccaat
     7981 gcccgccgcc gttgttcctc acggctccgc gaacggcgtc cggcaccgcc gcgaggtcct
     8041 tgataagggc ctcgatcgtc ttggcttcga gggccgcatt gccggcgatg gccttgttga
     8101 ggttggtgac gtaggcctgg tgatgcttgc cgtggtgaat ctccatcgtc tgagcgtcga
     8161 tgtgaggttc cagggcttcc ttcgcgtagg ggagtggggg cagttgatgt ggcataagct
     8221 ttgattcaat ggtctcgtgc actcgtttac ggcttcaatt ccgccatgga tgcgaggcgc
     8281 gtccactcac atgcgccggg tcaaacgcgg tgcattcgcc cgcgaacccg caactcgcag
     8341 attgtcggcg tcgggcgaga atcgccctcg gcgttctgtg tgtgacgtgt catcgctctt
     8401 gcatcagtcg tgggcaacat agcagggcat cggttgccgc acaagcgacc aggtgctggt
     8461 ttgcaggcgt cccgcgacac gggatgagtc ggcgctggcg cctgtctttc gcggggcgtc
     8521 gacgcccgcg ggacgaacgc aacccacggt ccttctgttt tccgtggcca gcggttggtg
     8581 tttcaggata gagtgcgctc cgtgcgttgc gccgtgttgt gccgggtttc ggctcttgtt
     8641 ctggctggct tgctgctctg ttcgtgcggc aagcctgcgg cgggttcgaa ggcgcgacag
     8701 aacggggctg aacccgcgcg cccggtgcgg ctggcggctg tcgaggtgcg tctgatggag
     8761 cggagcatca aggcgacggg cacgctggcg gcttacgagg agtcgacgct cagcgcgaaa
     8821 gtgcccgggc gtttgcagca actgatggtg gacatcggga gcgtggtgcg ggcgggggat
     8881 ctgctggccc aggtcgagcc gcgggattat gaactgcggc tgcagcaggc gggtgccgcc
     8941 ctggctcagg cgcgcgtggc gctgggtttg cctctggatg gcgacgatga cgagatcgcg
     9001 cttgacgagg tgagttcggt caaacaggcc aaggccgtgc tcgacgaggc ctcgaagaac
     9061 caagaccgcg tggcccgtct tctcaagtcc ggcatcgcct cggacgcgga gttcgacacg
     9121 gtcgaggcga cccacacggt ggctgcggcc aagtacgcga cggcgctgga agaggcgcgg
     9181 atgcggatgg cgacgctgtc tcagcgccgg gccgagtatg aaatcgcgcg caaacaactc
     9241 gccgacgcct ccgttcggac cccattcgac ggctcggtac agtcgcgtcc ggccagcgtc
     9301 ggcgagtacg tggcgacggg tgcgccgatc gtgaccgtgg tcaggaccga tccgctacgc
     9361 ctgcgtctgg tggttcccga gcgggagtca atgcttgtcc gagccgggca aacggtcagg
     9421 ctgcgtgtgg aaggggacgc gacgacgtat cccgggacca tcacccgtct gagtcccgcg
     9481 ttggtcgagg aggaccggat gctgcgggtc gaggctgacg ttcccaatcc cgggtctctg
     9541 cgaccgggga tgttcgccag cgcggtgatc gtggtggacg accggcagga ggcgctgacc
     9601 gtgccgacca acgccgtcgt gacatttgcc ggcatcgaaa aggtggtgct tgtccaggat
     9661 gacaaggcgc tcgaaaagga catcgccacc ggacgccagc gcatggatcg gatcgaggtc
     9721 ctgtccgggc tcaccctcgg gcagacggtc gtgttggatc cggccggcct gcggactggc
     9781 cagcgtgtca ctgtggaatc gaccctgagc gagacgccgt cggcgcccgc agttggcggt
     9841 cagtccaccg ccacgggggc ggggggcgac gcgggtgcag aggcggcgcg gagcgaattg
     9901 ggcgcggcga acagacagtc caatgcagaa gctcgctgaa atctgcatcc ggcggccggt
     9961 gttcgccacg atgatcgtga tggcgttggt tgtcgtgggc gtggccgggt acgtgaatct
    10021 gggggtggac cggtttccgt cggtcgatct gcccaccgtg cgcatcatga ctcgtctgcc
    10081 tggcgcatcg cctgaagaaa tggaatcgct ggtgtcgcag ccgatcgagg aggttgtgaa
    10141 caccatcgag gggattcagg aattgcgctc ggtgagcggg aacggttcct ccttcgttat
    10201 cgtgaccttc gaactcaacc gcgacatcga cgtcgcggct caggatgtgc gggacagggt
    10261 ctcgaccgtc ttgcgccacc ttccggacga caccgacccg ccgacgattg ccaagaccga
    10321 cgcagaccag gcgccgatca tgaccttggc ggtgtcgggt ccgcgttcga ggcgcgagtt
    10381 gaccgaaatc gcggacaaga tcatcaagac ccagattgag cgttcgctcg gggtggggga
    10441 ggtgaatgtc tatgggggtc tcgagcgggc gatcaacatc tgggtcgatg ccgatcgtct
    10501 ggcggcgtac cggatcccga tcacggcggt gcgggaggct ttggtgcggc agaacgcgaa
    10561 catacccggc ggcaacgtga ccgggccgtt gcgcgagcac acgctgcgca ccataggccg
    10621 gctgaacgac cccagggcct tcaacgatct ggtgatcgcc acccgcaacg gctcgcccat
    10681 ccgcattcgg gacatcggca tggccgaaga cggcacgaag gagcagcgct cggtctcgcg
    10741 gttggacggg gtgccgacgg tgtcgatgga gattgtgcgg caatcggggg caaacaccgt
    10801 tgcggtcatc gaggcggtga aggccaatct cgaagtgctg agggcgcagc ttccgtccga
    10861 tgtgcgggtc gaagtgatcc gcgaccagtc ccgctacatt tacgaggccc tccacgagat
    10921 tcggcagcat ctgatccttg gcagcatcct ggcctgcctg gtcgtgctcc tgttcatgcg
    10981 caacggacgg gcgatggtga ttgcggcggt ggcgatcccg acctcggtga tcgccacctt
    11041 cggcatgatg aaggcgttgg atttcacgct caacagcgtc accatgctcg ccctggtgct
    11101 catggtgggc atcgtgatcg acgacgccat cgttgtcctc gagaatatct tccgattcgt
    11161 cgaggagaag cggatgcgtc cgttcgaggc ggcgcgggcg gcgacggcgg agatcgggct
    11221 tgctgtgatg gccacgacgt tcagtctggt ggtgattttc gtgccggttt ctttcatgtc
    11281 gagcatctcg ggtcgattcc tctatcagtt cgggatcacc gcggccgtgg cggtgctcgt
    11341 gagcctcctg gtgtcgttca ccctcacgcc gatgatgagc gcccggctgt tgcacgtgaa
    11401 tggcgggggc gatcagggcg ccgcaccccg ttcccgcagc ggcttctatc attggatcga
    11461 ccaggtctac acggcggctt tggcctgggc ggtgggccgg cgttggctgg ttgccgccct
    11521 ggccctggcg gtcattgtca cgtccgtgcc gctctatcaa gccgtgcggc aggagtatct
    11581 ccccagcaac gtggacgagg gggaattcga tgtgcgtgtg acgaccgccg aaggcaccgg
    11641 actggtctcg atgaacgagg tgtccgagcg catcgagaag gagctgctcg gtacgccggg
    11701 cgtcgacctt gttctttcgt cgaccggcag cggtttcatg ggcagcgtca acaacgccgg
    11761 cttctttgtc cgactcaagc cgcacgccga acggacgttc cgctggacgc gcctctttca
    11821 gtggccgccc tggcgggcat tcgagaacaa ctacacgcag cgcgacatcc agcggatgat
    11881 ccgcgcccgc cttgcgggca tccccagcat tcgattccag gtgcgcaatc cgcagacatt
    11941 cgtcggcggc ggcccgaact tcgacatcga tttcgcgctg ctcggtccgg atctgacgac
    12001 tctctggcgc tacgccgagg ccctgcgcga gaaggcgccc gaactcgggc tcttggatgc
    12061 cgataccacg ctcaaactcg acacgcccga actccgcgtc gagatcgacc gcgaccgggc
    12121 ggcgaacctg ggcgtggata cccaggatat cgcgaccgcg ctgcggatca tggtcggagg
    12181 cgacaccgag gtgacccgat ttcgcgacga aagcatgaac gaggattacg atgtcgccat
    12241 ccggctcaaa gagggcgacc gcaatgaccc ggcgaccatc gccagtctgt tcgtgccgcg
    12301 gcagggcggg ttggtccgac tcgacaatgt agtcaagctg gtgcccacac agagtccgtc
    12361 gcggatcgac cggttggacc gtcagcgcca ggtcagcctg cgggcttccg tggcgcccgg
    12421 gttcgcccag gccgaccgca tcgccgcgct gcgggcggag gtggcgaaga tggggctgcc
    12481 aaccggatac tcgacacgtg tttccggccg ggcgcgcgag ctcgagacca ctttcaacga
    12541 gtttctctgg gccttcgccc tctccgtggt cctcatgtac atcatcctgg cgtctcagtt
    12601 cgaaagcctt gtccatcctt tcacgattct cctctcgctg ccgttgtcgg tgccttttgc
    12661 ctttctttcg ctcctgattg ccggtcagac gatcaacctc tactcggcgc tggggttgct
    12721 cgtcctgttc ggtgtcgtca agaagaacgc cattctccag atcgatcaca tgaacaagct
    12781 gcgggcagcg gggctcgacc gggcggctgc cattctgcgc ggcaaccgcg cgcgcttgcg
    12841 ccctatcctg atgaccacca tgacgctgat cgccggcatg actccgctgg ctctcgggac
    12901 gggtccgggg gcggccgagc
//
LOCUS       DAOQRO010000050        12794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4002_length_12794_cov_4.462752, whole genome shotgun sequence.
ACCESSION   DAOQRO010000050 DAOQRO010000000
VERSION     DAOQRO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4002_length_12794_cov_4.462752"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /gene="prsK"
                     /locus_tag="PKK20_03200"
     CDS             <1..1583
                     /gene="prsK"
                     /locus_tag="PKK20_03200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNU98926.1"
                     /translation="GRMYVSSQAVLFSGIQLPVVGVLGTSLLLGTLLLAFSFLRTGLS
                     EVDVYPSHAFLYGSLTIVLVGVYLLSVGVLAEIVARFGGDQAFALKALLLLLTLVGLA
                     VVLMSDRVRHRTQQFISRHLRRPVYDYRRVWAAFTERTTSRLDQADLCRAVAMLIAET
                     FNTLSVTVWVVDPHRQQLRFGASTALTEQAANELLDLGDDSAAVIAAVHAAPTPLDLS
                     SAEGGWVETLKRCNPDHFKKGGNRVCVPLVAAGEVLGLITLADRVSGVQFSHEDYDLL
                     KCIGDQVAASLLNLQLSRKLIEARELEAFQSMSAFFVHDLKNTASTLSLMLRNLPVHF
                     NDPEFRADALRGIGASVDRLNDLVQRLALIRRGLEVKPVLADLNQLVKAALGQIDGAD
                     SIEVIQELQSVPRLPVDLEQLPKVLTNLLLNAREAISGSGTIRVATMVQGGHAVVSVA
                     DTGCGMSAEFLSRRLFRPFQTTKKKGLGIGMFQSKMIIEAHGGRVEVASEPGRGTTFR
                     VVLPLSSERTVAGSGSLS"
     gene            1805..3193
                     /locus_tag="PKK20_03205"
     CDS             1805..3193
                     /locus_tag="PKK20_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNU98927.1"
                     /translation="MKPTVLIIDDDEAVLDQLKWALADEYEVLLAKDRPSAIETFRAR
                     RPDLALLDLGLPPLPNDPREGMAALADMRNLDRHAKVIVISGLSGKETAARAVGEGAC
                     DFLLKPVQIDSLKLLLSHCRHVVTLKREYEEIRQHTEVEPFELLQSRSPRMEELLENI
                     RKVATAEAPVLILGESGTGKDVVALAIHRQSGRRVGPFVKLNCDMASESLLESELFGR
                     EDDARSDGASAEGRLESASGGSLFLQDVGDLPLRSQVRLMRLIGERVADGVKERPSGE
                     TAPRLIAASSKDLTVAIAKGNFIEGLYYRLAVLVIRVPALRDRKEDIRQLAEAFLKRF
                     AAQFDRPNLQFDSEALRALDRHAWPGNLRELENCVRRAAIMSDTDILRADDLGLTPSH
                     AQPAAAGLREARQSAERAVIIGALRKHHGKITAAAMELGISRPTFYSLMEKLGIERRS
                     LIWDHSEETSEE"
     gene            3197..3829
                     /locus_tag="PKK20_03210"
     CDS             3197..3829
                     /locus_tag="PKK20_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNU98928.1"
                     /translation="MRQTTTDVGRASFAWAVLTLALVVMPSGCQSHRPTFISPADAEP
                     DRTIVLREGDVVKVTFPGSPNLNTTQQIRRDGNINLALVGDLKASDLTPSALEKEILK
                     VYGNQLLAPEVTVSLESTAFSVFVTGMVLRPGKVTSDRPITLLEAIMESGGFDYTRAN
                     LKAVRVMRRDGERMKTFIIDLKPVMAGGPALPFHLRPSDIVYVPERFSLF"
     gene            3838..5223
                     /gene="prsR"
                     /locus_tag="PKK20_03215"
     CDS             3838..5223
                     /gene="prsR"
                     /locus_tag="PKK20_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNU98929.1"
                     /translation="MQSHLLIVDDDDEIRSQLKWALGGAYALLLAGDRARALEQFRTC
                     RPMVVLLDLGLPPNPDSPEEGLRVLADLLSVEKLTKVIIVTGQGEKHNALRAVGAGAY
                     DFLSKPVDLDELKVILRRAFHLARIEREYRDLQRRLNEDGFEDMLGASPSMQQLFTTI
                     RKVAATDAPVLILGESGTGKEKAALAIHRRSQRKDGPFVPINCSAIPETLLESELFGH
                     EKGAFTGAHTQRKGRIQSAIGGTLFLDEIGELPLAIQVKLLRYLQEHRIERVGGRVPI
                     EVDARVIAATNADLERAIRESKFREDLFYRLAVVVLHLPPLRHRGGDLLLMGRVFLNK
                     FAVEFGRDSAGFSPQALAAMQGHSWPGNVRELENRIKRAVIMADGPRITPEDLELTTA
                     VSSGQSPRTLQQARETAEREVIQEALRRNGGKMTAVATELDISRPTLYELIEKLAIER
                     PDRKGESTPAE"
     gene            5360..8602
                     /locus_tag="PKK20_03220"
     CDS             5360..8602
                     /locus_tag="PKK20_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU98930.1"
                     /translation="MNNANRSLLSVLTIAALLGAAGCSREAKRDRHYSRAEKYFASEQ
                     YEKAILEYRNVLRLDPTNRTALLRVARIFFEQGSPVQAMQAMQAADPHLESDDPALRC
                     RLAEFRLAMGDTVRGRNELLDLLAQSPTNSDALQLLAGPGTPPQELTNTMVYLEQLDA
                     KHPNSAAVLLARAALLIRQRQLDTAEQFVQRALQLDPNQAQAHVQMGQIHLLRTNLAA
                     ADEAFGKAVKLAPLRSPVRTAYAQSKMVSGKPDEARALLEDVLSQAPDYLPAALLLAR
                     LDMQEKRLDEALALTGRIVSKDPLNIEARQLRTELHKAKGQPEKAALEWEQFDQSVWP
                     NPFIKLQVARAYLSGGERVKAGAALDQAIIAGSNRLDTVAIEARLLRAQLHVEGRTPA
                     VAVESLQRLLSITNVLEARILLIDAQRGAGRLDDAVATCQGLIRDYPDRPGFPMMLGM
                     LLREQGGVADARNAFRRELELSPGNPMALYQLVDLEIAATNAPAALKTVQTELARTNS
                     SALKYLEGRALAAQRLWPQAQASLEESISLNPQFSLAYQLLADIHIANTNLTGAARRL
                     EELVSKKTNDVQSMAILGTLYERMDQPELARAKYEQILEINPQFVPALNNLAYLYTER
                     LPQLDRAHEFAQKARQLAPEDPSVMDTLAWVLHKRGEHQQALPLLREAAQRVPGNAEI
                     QYHLGMVSYMLGQPDAARAALERAVAAPQSFPSKAEAQRQLALLASGAAASGTEDRAT
                     LEARVKDQPKNLTARSSLAAVYEQEGQFPKAATEYEEILRQNPKAAQASLRLAELNAG
                     PLNDPAKALEMARKAHELAPNDPRAAGLLGRLLARRGDHVQAYGLLQSAAQRSPQQAD
                     LQYDYAWAAYAMGRVADAHQAMRRALEIQPNFQAVDAARDFVAMTALALDPQGLAQAE
                     PRIREILEADPDHVPALMAQAALFRARADGKAAAALYERVLRVFPRFAPASRELGLVL
                     AGDPAQGQRAYDLLSRTREAFPDDGAVARALALLSYARKDYRYTTTLIESYVRRHPDD
                     SEALFYLGMAQCQIKQTDQGKEQLRKAIAAGLKEPLLAEARKALAAPSSQ"
     gene            complement(8752..11946)
                     /locus_tag="PKK20_03225"
     CDS             complement(8752..11946)
                     /locus_tag="PKK20_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNU98931.1"
                     /translation="MGCVDLTSDLLARVAGWRVMQEARALLAGGNVQESSWAPPRLEG
                     VVREGGTKYRTGLVLHSAIDVDTLCSCRRARIEGAICIHAVAAGLHWLATRQAAPHPI
                     HPSPPAREPTATTVRKDAASGHPGSRATEPQPRRALRRAAPGEAAEEIEIRIIFPPNL
                     EQAMAKGKVMLVFEAVRSGRCVPLNMLPLDAPFLLSPADASALDTIETLTADGVPAML
                     LLGGSDLGQLLPKLAGHSGLSLGRGRPLEISLDAISWPVRATLEASGQITLRLDGSVP
                     TVLATGEVDWCLRENRLQPLRLPTAARGLARGPIRLERSRVPAFLGQDWPVLVADGHV
                     QADFDLSDFGLETEPPRFRLRLEGSLARIEARLTCDYAGHAVAPIHGVVEPLCFPDAA
                     CPTRYTLRDSGAEHAALERLAQWGFTGPDRNNRFLLDGETAVLNFFARVFPRLQREWD
                     IELEPRLDGAAKTRLERIEPELLVMPSGEQWFDLSVAFASAGGERFSAADIQRLVLAG
                     QSHVRLRNGRLALIDTGAVEELQQVLLDCEPQQTTSGYRLANAQADFVQSTLREHAGW
                     QLEAPRAWLDRTARPADAAAHECPPLGSLDAVLRPYQKQGVAWLWFLRKHGFGGILAD
                     EMGLGKTLQTLAVVAAAQAQTEPGSGPLPSLVVCPTSLVFNWVAEAARFVPGLRVLPL
                     HGLERQARFAEIPRHDLVVTSYALIRRDLDRYRALEFDTVVLDEAQHIKNRETQNAQA
                     VKAIRARHRLVLTGTPLENSVLDLWSIFDFLMPGYLGKARDFRERYEIPITRDRDAAV
                     RDRLARRVRAFLLRRVKRDVAPDLPEKIEQVALCDLTSDQATVYRQVLETGRREIVET
                     VGVQGLARSRMIILTTLLRLRQVCCDLRLLDLPGVEGAAPSGKLDLFEELIEEVIDGG
                     HRALVFSQFTSMLALIRDRLTDRGIEFCYLDGATPDRGGVVRHFQDHTDIPVFLISLK
                     AGGAGLNLTGADTVIHFDPWWNPAVEAQATDRAHRLGQKRVVTSYRLIARGTVEEKIL
                     GLQSRKREVIDALLGGEDQPGGDLTWEEIQDLLA"
     gene            complement(12032..12745)
                     /locus_tag="PKK20_03230"
     CDS             complement(12032..12745)
                     /locus_tag="PKK20_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98932.1"
                     /translation="MPLPEVNAPPRPVESASATDLMVALSRLVRGLSALFWGLPLALV
                     VSVQTARTNLLAGLGLLPPLLANGLLCYALHQMRHFQRSERVWQHARERALVFASINV
                     GLSPFLYWWRVVPDVLHFQFAVLVLALSGLMLLFTVNQMLQRLTAMLPDETLRIEARL
                     FTTLNLYLLLASALLGAGWVGLTQIEQPSLALSMMRDIATRVGVLLMLLLVLLPLALT
                     MTLIWRIKETILASVFTSG"
BASE COUNT         2428 a         3910 c         4195 g         2261 t
ORIGIN      
        1 gaggccggat gtacgtcagc agccaggcgg tgttgttctc gggaatccaa ctcccggttg
       61 tcggcgtgtt gggcacgagt ctgctgttgg gaacgctgct gctggcgttc tccttcctga
      121 ggaccggttt gagcgaggtc gacgtctatc cgtcgcacgc gtttctgtac ggatcgctga
      181 cgattgtgtt ggtcggggtg tatttgttga gcgtcggtgt gctggccgaa atcgtcgcgc
      241 ggtttggggg cgaccaggcc tttgcgctca aagcgttgct gttgctgcta actttggtgg
      301 ggttggcggt cgtgttgatg tccgaccgcg ttcggcaccg gacgcagcaa ttcatcagtc
      361 ggcatttgcg gcgccctgtc tacgattatc gccgcgtctg ggcggccttc accgaacgca
      421 cgacgtcccg gcttgaccag gcggatttgt gccgcgccgt ggcgatgctg attgccgaga
      481 cgttcaatac actgtcggtc acggtttggg tggtcgatcc gcatcggcag caattgcgct
      541 ttggcgcatc gacggctttg accgagcaag ccgcaaacga actgctcgat ttgggagacg
      601 acagcgcggc ggtgatcgcg gccgtgcacg cggcgccgac gcccttggac ctcagtagtg
      661 ccgagggcgg ctgggttgag accctgaagc ggtgcaatcc ggaccatttc aagaaaggcg
      721 ggaatcgggt ctgcgtgccg ctggtggccg cgggcgaggt gcttgggctc atcacgctgg
      781 ccgatcgggt cagcggcgtg caattctcgc atgaggatta cgatttgctg aagtgcatcg
      841 gggatcaagt ggccgcgagt cttctcaatc tgcagttatc ccggaaactg atcgaggcaa
      901 gagaactcga agcgtttcag tcgatgtcgg ccttcttcgt tcacgacctg aagaacacgg
      961 cgtcgacgct ctcgttgatg ttgcggaacc tgccggtgca tttcaacgat cctgagttca
     1021 gagcggatgc tctgcgcggg attggggctt cagtagatcg actcaatgac ctggtccagc
     1081 gccttgcttt gatccgccgc gggctcgagg tgaagccggt gctggcggat ttgaaccagt
     1141 tggtcaaggc tgcgctggga cagatcgatg gtgcggattc gatcgaggtg attcaggagt
     1201 tgcagtcggt tcctcgattg ccggtcgatc tcgagcagtt gcccaaggtt ctcacgaatc
     1261 tgttgctcaa cgcccgtgaa gcgatttcag gttccggaac cattcgtgtg gcgacgatgg
     1321 tccagggcgg gcacgcggtt gtctcggtgg cggacacggg ctgcggtatg agcgcggagt
     1381 tcttgagccg gcggctcttt cgaccctttc aaacgaccaa gaagaagggg ctggggattg
     1441 ggatgttcca gagcaagatg atcattgagg cgcatggcgg gagagtcgag gtcgcgagcg
     1501 agcctggtcg cggcacgacg ttccgtgtgg tgttgccgct gagttcggag cgcaccgtgg
     1561 ccgggagcgg ctcactcagc taatgcgctc tcggtgaatc tgggggtgac tcgagatcct
     1621 gattctcgcc ggcgacagga gattacgatt acgattagga atacgattta gaataggatc
     1681 aggagacctc gatgcgagtt cagtgggctg ttcaagcggg cactcgtttt attgcctgcg
     1741 tgcgatcatc ccaagaacct gccgcggggt cctgcgacag aaccggatcg aacgactttt
     1801 ttcaatgaaa cccacggtat taatcatcga cgacgacgag gctgtcctcg atcagttgaa
     1861 atgggcccta gccgacgaat acgaagtctt gctggccaag gaccggccga gcgccatcga
     1921 gacatttcgg gcgcggcgtc cggatttggc gttgctcgac ctcggtctgc cgccgttacc
     1981 gaacgatcca cgcgaaggga tggctgccct ggcggacatg cgaaatctgg accggcatgc
     2041 gaaggtgatt gtcatcagcg gtctgagcgg caaagagaca gcggcgcggg cggtcggtga
     2101 aggggcctgc gatttcctgc tcaaaccggt gcaaatcgac agcctgaagc tgcttctgag
     2161 ccactgccgc cacgtggtga ccctcaaacg cgaatacgag gagatccggc agcataccga
     2221 ggtcgagccg ttcgagcttc ttcaaagtcg aagcccgcgg atggaggaac tccttgagaa
     2281 catccgcaag gtggctacgg cggaggcgcc ggtgctgatc ctgggcgaga gcggcacggg
     2341 gaaggacgtg gtggccctgg cgattcaccg gcagagcggg cgtcgggtcg gaccgttcgt
     2401 gaaactcaac tgcgacatgg cgtccgagag ccttttggag agcgaactgt tcgggcgcga
     2461 ggacgatgcg cggagcgacg gggcgtccgc ggaaggtcgg ctcgagtcgg cgtccggcgg
     2521 ctctctcttt cttcaggatg tgggcgactt gccgctgagg tcgcaggttc gactcatgcg
     2581 gttgatcggg gagcgggtgg cggatggagt gaaagagcgg ccttccgggg agaccgcgcc
     2641 gcggctcatt gctgcatcga gcaaggatct gaccgtcgca atcgccaagg gcaacttcat
     2701 cgagggcctg tattaccgac tggccgtgct ggtgatccgg gtccccgctc tgcgcgaccg
     2761 caaggaggac atccggcagt tggccgaggc gttcttgaag cgctttgcgg cgcagtttga
     2821 ccggccgaat ctgcaattcg acagcgaggc gttgcgcgcg ctcgatcggc acgcgtggcc
     2881 gggcaatctg cgcgaacttg agaactgcgt gcgacgcgcg gcaatcatgt cggacaccga
     2941 catcctgagg gcggacgatc ttgggctgac gccatcgcac gcacagcctg cggccgccgg
     3001 tttgcgcgag gcccgtcaaa gcgccgagag ggcggtgatc atcggggcgc tgcggaagca
     3061 ccatggcaag atcacggcag ccgcgatgga gttgggcatc agcagaccca cgttctactc
     3121 gttgatggag aaactgggca tcgaacgtcg aagtcttatc tgggaccatt cggaggaaac
     3181 aagcgaggaa tagacaatga gacagacaac aacggacgtc ggaagagcat cgtttgcttg
     3241 ggcggtcctg actctggcgc tggtcgtcat gccttcaggc tgccaatcgc accgccccac
     3301 cttcatcagt ccggccgacg ccgagccgga ccggacgata gtgctgcgcg agggggatgt
     3361 cgtgaaggtc acgttcccgg gaagcccgaa cttgaacacg acgcagcaga ttcggcgcga
     3421 cggcaacatc aacctggcat tggtcgggga tctgaaggca tccgatttaa caccgagtgc
     3481 cctcgagaag gagatcctca aggtgtacgg gaaccagttg ctcgcccccg aggtgaccgt
     3541 cagtctcgag tccacggcgt tctcggtctt tgtcaccggc atggtgttgc gaccgggcaa
     3601 agtgacatcg gaccgcccga tcaccctgct cgaggccatc atggagtcag gcggcttcga
     3661 ttacacacgc gccaacctga aggccgtgcg ggtgatgcgt cgcgatgggg agcggatgaa
     3721 gaccttcatc atcgatctga aacccgtgat ggcgggcggg cccgcgctac cgtttcacct
     3781 tcgcccgtcg gacatcgtgt acgtgcccga acggttttcg ttgttctaaa tgccgatatg
     3841 caatcccacc tgctgattgt tgacgacgac gacgaaatcc gctcccagct caaatgggcc
     3901 ctgggcgggg cctacgcgct tctgttggcg ggggatcgtg cgagagcact cgaacaattt
     3961 cgaacgtgtc gtccaatggt tgtactgctc gatctagggc tgccgcccaa cccggactca
     4021 ccggaggagg ggctgagggt ccttgccgat ctgctttcgg tcgagaaact gaccaaggtg
     4081 atcatcgtga ccggccaagg cgaaaagcac aatgccctca gggcggtcgg ggccggcgcc
     4141 tacgattttc tgtccaaacc ggtcgatctc gacgaattga aggtcatcct tcgtcgtgcc
     4201 tttcaccttg cccggatcga gcgcgaatac cgggacctgc aacgccggct caatgaggac
     4261 ggattcgaag acatgctggg cgcgagcccg tcgatgcaac aactgttcac caccatccgc
     4321 aaggttgctg cgaccgatgc cccggtgttg attctcggcg agagcggcac gggcaaggag
     4381 aaggctgcgc tggcgatcca tcgccgcagc cagcgcaagg acggtccgtt cgttccgatc
     4441 aattgcagcg ccatcccgga gacccttctc gaaagcgagt tgttcggaca cgagaagggc
     4501 gcctttaccg gcgcccacac ccagcgcaaa ggccggatcc aatctgccat tggaggcacg
     4561 ctttttctgg atgagatcgg cgagttgccg ctggcgatcc aggtgaagct gttgcgctat
     4621 cttcaggaac acaggatcga gcgggtgggc ggccgggtgc cgatcgaggt cgatgcgcgt
     4681 gtgatcgccg ccacgaacgc cgacttggag cgggcgattc gcgagagcaa gtttcgcgag
     4741 gacctcttct atcgtcttgc tgtcgtggtg ttgcatctgc cgccattgcg gcatcggggc
     4801 ggggatctgt tgttgatggg acgggtcttt ctgaacaagt tcgcggtcga gtttgggcgg
     4861 gattcggctg gtttcagccc gcaggccctg gcggccatgc agggccattc ctggcccggc
     4921 aacgtgcgcg aactcgagaa tcgcatcaag cgggccgtga tcatggccga cggtccccgc
     4981 atcacgcccg aagacctcga gttgacgact gcggtctcgt ccggccagag cccgcgcacc
     5041 cttcagcagg cgcgggagac cgcggaacgc gaggtgatcc aggaggctct gcggcgcaat
     5101 gggggcaaga tgaccgccgt cgcgaccgaa ctcgatatca gccgtcccac gctctatgaa
     5161 ctgatcgaga agctcgcgat cgaacgcccg gatcggaagg gcgagtcaac gcctgcggaa
     5221 tagaattacg aatacgatca ggagcggata gactccggtt gattgtccac gcgcgctttg
     5281 ctaaactatt gaatgtcaac gatcaaggct taacactcaa acctgagagc accatgccgc
     5341 tccaacccga gcaccctgaa tgaataacgc aaatcgaagt ctcctgtccg tgctgacgat
     5401 cgccgctctc ctgggagcgg ccggatgctc ccgcgaggca aaacgcgacc ggcactacag
     5461 ccgggcggag aagtacttcg catcggagca atacgaaaag gcgatcctcg agtaccgcaa
     5521 cgtgttgcgg ttggatccga ccaatcgcac ggcgctgctg cgcgtcgcgc gcatcttctt
     5581 cgagcaaggc tcgccggtgc aggccatgca ggccatgcag gccgccgatc cgcatctcga
     5641 atcggacgat cctgcgctgc gttgccggct cgccgagttt cggctggcga tgggggacac
     5701 cgttcgagga cgcaatgaac tgctcgatct gctggcgcag tctccaacca acagcgatgc
     5761 cttgcaattg ctggccgggc ccggcacccc gccccaggaa ctgacaaaca cgatggtcta
     5821 cctggagcaa ctcgacgcca agcatcccaa ctccgcggcg gttcttctgg ctcgtgcggc
     5881 gctcctgatc cggcagaggc agctcgacac agccgaacag ttcgtgcagc gcgcgttgca
     5941 gttggatccg aatcaggcgc aggcgcatgt gcagatgggg cagattcatc tgctgcggac
     6001 caacctggct gccgccgacg aagcgttcgg aaaggctgtc aaactcgcgc cgctgcgctc
     6061 gcccgtgcgc acagcctacg cccagagcaa gatggtgtcc ggtaaaccgg acgaagcgcg
     6121 ggcgctgctc gaggatgtgt tgagccaggc gcccgattac cttccggccg cgctgctgct
     6181 ggcgcgtctc gatatgcagg agaagaggct cgatgaagcc ctcgcgctga ccggacgcat
     6241 cgtgtcgaag gacccgctga acatcgaggc gcgccagttg cgcacggaat tgcacaaagc
     6301 caagggccag ccggagaaag cggcgctcga atgggaacaa ttcgaccaga gcgtgtggcc
     6361 caacccgttc atcaagttgc aggtcgcgcg tgcttacctg tctggcggcg aacgcgtcaa
     6421 agcgggcgcc gcgctcgacc aggccatcat cgccggctcg aacaggctcg atacggttgc
     6481 catcgaggcc cgtctcttgc gcgctcaact gcatgtcgaa ggccgcaccc cggccgtcgc
     6541 cgtcgaatct ctgcagcgat tgctcagcat caccaacgtc ctcgaggcgc ggattctcct
     6601 gatcgatgcc cagcgcgggg cggggcggct cgacgacgcg gtggccacct gccagggact
     6661 gattcgcgac tatccggaca ggccgggatt tcccatgatg ctggggatgc tgctgcggga
     6721 gcaggggggg gtggcggacg ctcgaaacgc gttccgccgt gaactcgagc tttcaccggg
     6781 gaacccgatg gccctttacc agctcgtcga tctcgagatt gccgccacca acgcgccggc
     6841 tgcgctcaag acggtgcaga ccgagctggc ccggacgaac tcgtcggctc tgaagtacct
     6901 cgaaggtcgg gcgcttgcgg cccagcgcct ttggcctcag gcgcaggcat ccctcgagga
     6961 gtccatcagc ctcaacccac aattctcgct ggcgtaccaa ttgctggccg acatccatat
     7021 cgccaacacc aacctgaccg gggcagcccg gcggctcgag gaattggtat cgaagaagac
     7081 aaacgatgtt cagtcgatgg cgattctcgg gacgctttac gagcggatgg atcaacccga
     7141 actggcgcgt gccaagtacg agcagattct cgaaatcaac ccgcagttcg ttcccgcgct
     7201 aaacaacctg gcttacctgt acacagagcg gctgccgcaa ctcgatcgcg cgcatgaatt
     7261 cgcccaaaag gcgcgccaat tggcgcccga agatccttcg gtcatggata cgttggcctg
     7321 ggttctgcac aagcgcggcg agcaccagca ggccttgcca ttgctgcgcg aggcggccca
     7381 gagagtcccc ggcaacgcgg agattcaata ccacctggga atggtcagct acatgctcgg
     7441 ccaaccggac gccgcccggg ctgctctcga acgggccgtg gccgcgccgc agtctttccc
     7501 gagcaaagcc gaggcccagc gtcaactcgc cctgcttgcg tccggagccg cggccagtgg
     7561 cacggaggac agggcgaccc tcgaggcacg agtcaaggac cagcccaaga atctcaccgc
     7621 ccgttccagc ctcgccgcag tttacgagca ggagggtcag tttccgaaag ccgcgactga
     7681 atacgaggag atccttcggc agaatcccaa ggcggcgcag gcgagtctgc gcctggccga
     7741 actgaacgcc gggccattga acgacccggc caaggcgctc gagatggccc gcaaggcgca
     7801 cgaactcgcc cccaacgatc cgcgggcagc gggtttgctc gggcgcctcc tggctcggcg
     7861 cggcgatcac gtccaggcgt atgggctgct gcaatccgcc gcgcagcgaa gtccgcagca
     7921 ggccgatctg caatatgact acgcgtgggc ggcctacgcc atgggacggg tggcagatgc
     7981 acaccaagcc atgcgacgcg ccctcgaaat ccagccgaac ttccaggcgg tcgatgccgc
     8041 acgcgatttc gtcgcgatga cggcccttgc gctcgatccg caggggctag cccaagcaga
     8101 gccgcggatt cgggagattc tcgaggctga tccggatcat gtgcctgcct tgatggccca
     8161 agcggctctg tttcgcgcgc gggctgacgg caaggcggcg gcggcgcttt acgagcgggt
     8221 gcttcgggtg ttcccgaggt ttgcgccggc cagtcgcgag ctggggctgg tgttggcggg
     8281 cgatccggcc caggggcaga gagcctacga tctgctctcg aggacgcgcg aggcctttcc
     8341 ggacgatggc gccgtggctc gggccctggc gttgctgagc tacgctcgca aggactaccg
     8401 ttacacaacc acactgatcg agtcctatgt ccggaggcac cccgacgaca gcgaggccct
     8461 gttctacctc ggcatggccc aatgccaaat caagcagacc gatcagggca aggaacaact
     8521 tcggaaggcg attgcggccg ggttgaagga gccgctgctg gctgaggcta gaaaggcctt
     8581 ggccgctccc tcttcccaat aaagccgctc ccacccaacc ccagacaacc atccacacag
     8641 cccgcaggtc cacctgcggg cttttttttg tctgattcct gcctttgacg gagcgagatg
     8701 gcgcgggctg atcccgcgtc gcgggacggc tgcggaaccg acggttcacg gtcaggcaag
     8761 cagatcctga atctcttccc aggtcaggtc gccacccggt tggtcctcgc cccccagcag
     8821 ggcgtcgatg acctcgcgtt tgcggctttg cagacccaga atcttctcct cgaccgttcc
     8881 ccgcgcgatc aagcgatagc ttgtcaccac gcgtttctgg cccagccgat gggcgcggtc
     8941 ggtcgcttgc gcttccaccg ccgggttcca ccaggggtcg aagtgaatca cagtatccgc
     9001 gccggtcagg ttcaggccgg ccccgccggc cttgaggctg atcaggaaga ccgggatatc
     9061 agtgtggtcc tggaaatggc ggacgacgcc gccccgatcc ggtgttgcgc catccagata
     9121 acagaactcg atccctcggt ccgtcagccg atcccggatg agggcgagca tcgaagtgaa
     9181 ctggctgaag accagcgcgc ggtgcccgcc gtcaatcact tcctcgatca attcctcgaa
     9241 caagtcgagc tttcccgagg gggcggcgcc ttcgacccca ggcaggtcga gaagccgcag
     9301 gtcgcagcac acctggcgga gccgcaacaa cgtggtgagg atgatcatgc ggcttcgagc
     9361 cagtccctgg acaccgactg tctcgacaat ctcgcgtcgc ccggtttcga gcacctggcg
     9421 ataaaccgtc gcctgatcgg acgtcaggtc acagagggcc acctgttcga tcttctccgg
     9481 aaggtccggc gccacatcgc gcttcacacg gcgcaggaga aacgcgcgga ctctgcgcgc
     9541 caggcgatcc cgcacggcgg catcgcgatc gcgcgtgatg gggatttcat agcgctcgcg
     9601 gaagtcccgc gctttgccca ggtaacccgg catcaggaaa tcgaagatcg accagagatc
     9661 gagcaccgag ttttcgagcg gcgtgccggt gagcaccagc cgatgccgcg cgcggattgc
     9721 tttcaccgcc tgggcgttct gtgtttcgcg attcttgatg tgctgcgcct cgtcgagcac
     9781 aaccgtgtcg aactcgagcg cgcgatagcg atcgagatcg cgccggatca gcgcgtagct
     9841 ggtgacgacc aggtcgtggc gcggaatctc cgcgaagcgc gcctggcgct cgagcccgtg
     9901 caacgggaga acccgcaggc cgggaacgaa tctggccgcc tcggcgaccc agttgaaaac
     9961 aaggctcgtc gggcagacca ccaggctggg caaggggcct gatcccggct cggtttgggc
    10021 ctgcgcggct gcgacaacag ccagcgtctg aagcgttttc cccagaccca tctcatcggc
    10081 cagaatcccg ccaaaaccgt gcttgcgcag gaaccacaac cacgccaccc cctgtttttg
    10141 ataagggcga agcacggcgt ccaagctccc caaaggaggg cattcgtgcg cggccgcatc
    10201 ggcgggtcga gcggtgcgat cgagccatgc gcggggcgct tcgagttgcc atcctgcgtg
    10261 ctcgcgtaat gtggactgga cgaaatccgc ctgcgcattt gccagccggt agcctgaggt
    10321 ggtttgttgg ggctcgcaat cgagcaacac ctgttgcagt tcctcgacag cgcccgtgtc
    10381 gatcaacgcc agtcgtccgt tgcggaggcg gacatggctt tggccggcca acacgagccg
    10441 ctgaatgtcc gccgcggaga atcgctcgcc gccagccgac gcaaatgcga ccgacagatc
    10501 gaaccactgt tcgccggaag gcatgacgag caattccggc tcgatccgtt cgagcctggt
    10561 cttcgccgcc ccatcgagcc gcggttccag ctcgatatcc cactcccgct gcaatcgggg
    10621 aaacacgcgc gcgaaaaagt tcaggacagc cgtctcgccg tccaggagaa aacggttgtt
    10681 ccgatccggc ccggtgaaac cccactgggc cagacgttcg agcgccgcgt gttcggcccc
    10741 ggaatcgcgc aaggtgtagc gcgttgggca cgcggcatcg gggaagcaga gcggctcgac
    10801 gacaccatgg atcggcgcca cggcgtgtcc cgcatagtcg catgtcagcc gggcttcaat
    10861 acgggcgaga ctgccttcga ggcgcagtcg gaagcgcggc ggctctgttt cgagaccaaa
    10921 gtccgacaga tcgaaatcgg cctgcacgtg accgtctgcg acaaggactg gccagtcctg
    10981 tccgaggaag gcaggcacac gcgatcgttc gaggcggatc ggtccgcgcg ccaatccccg
    11041 tgccgctgtg ggcaaccgga gcggctgcag ccggttctcg cgcaggcacc agtccacttc
    11101 gcctgtcgcc aacacagtgg ggaccgatcc atcgagtcgc agtgtgatct ggcccgaggc
    11161 ctcgagcgtc gcccgcaccg gccacgaaat cgcatcgagc gagatttcga gcgggcgtcc
    11221 gcgccccaga gacaacccgg aatgtccggc caacttgggt agcaactgtc ccaggtcgga
    11281 tccgccaagg agcagcatcg ccggtacccc gtctgccgtc agggtctcga tggtatcgag
    11341 tgcggacgca tcggctggac tgagcaggaa cggcgcatcg agcggcagca tattcagcgg
    11401 gacgcaacgc cctgagcgaa ccgcctcgaa caccaacatc actttccctt tcgccatcgc
    11461 ctgctcgagg ttgggcggga agatgatccg gatctcgatc tcctcggccg cttcgccggg
    11521 ggcggcgcgc cgcagggcac gccggggctg cggctccgtg gcgcgcgaac ccggatggcc
    11581 agacgcggca tcctttcgaa cggttgttgc tgtgggctcg cgtgcgggag gagaggggtg
    11641 gatcgggtga ggggcggcct gccgggtggc cagccaatgg agccccgcgg caacggcatg
    11701 gatacagatc gcgccctcga tgcgggcgcg ccgacaggag caaagggtgt cgacgtcaat
    11761 cgcgctgtgc agcacgaggc cggtccggta tttggtccca ccctcgcgga ccacgccctc
    11821 gaggcgcggc ggcgcccaac tcgactcctg gacattgccg ccggccagga gcgcgcgggc
    11881 ctcctgcatc acgcgccagc ccgccacccg cgcgagcaga tcggatgtca aatcgacaca
    11941 acccatgcgt gactttgctt tccttgcctg catgctgact tctcttgtgg actccggctt
    12001 cgcggtggca aaaagtggtg agaaatacag actagcccga ggtgaagacg ctggcgagga
    12061 tggtttcctt gatcctccag atcagggtca tggtcagcgc gagcgggagc agaacaagca
    12121 acaacatgag caacacgccc acccgggtgg cgatgtcgcg catcatcgac agcgcaagag
    12181 acggttgttc tatctgggtc aggccgaccc agcccgcgcc tagcaaagcc gaggcgagca
    12241 ggagataaag attgagcgtg gtgaaaagcc tggcctcgat tcgcagcgtc tcgtcgggca
    12301 gcatggccgt caggcgctgg agcatttgat tgaccgtgaa gaggagcatc agaccggaca
    12361 gggcaaggac caggacggcg aattgaaagt ggagcacatc cggcacgaca cgccaccaat
    12421 acaggaaagg cgagaggccg acgttgatcg aggcgaacac gagggcgcgc tctcgggcgt
    12481 gctgccacac ccgttcggag cgctggaagt ggcgcatctg gtggagcgca tagcagagga
    12541 ggccgttggc gagcaggggg ggaagcagac ccagaccggc gagaagattg gtgcgtgccg
    12601 tttgcaccga gacgaccaac gcgagtggca acccccagaa gagggctgac aagccgcgca
    12661 ccaggcggct gagcgccacc atcagatcgg tggccgatgc ggattcgacc gggcggggcg
    12721 gggcattgac ttcgggcagg ggcacgcgct gtcaatgctt ggccagatag gtgtactcct
    12781 tcaggccgtg ctcg
//
LOCUS       DAOQRO010000051        12760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4023_length_12760_cov_5.327194, whole genome shotgun sequence.
ACCESSION   DAOQRO010000051 DAOQRO010000000
VERSION     DAOQRO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4023_length_12760_cov_5.327194"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKK20_03235"
     CDS             complement(<1..856)
                     /locus_tag="PKK20_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98933.1"
                     /translation="MKCVLSRRQFLKATAVAGAATTWQLTPADVLGGTGSTAPSDRVT
                     LGVIGLGIQGIGDMRAFLGIPEVKVVAVCDVHATQRAAGKQVVDTFYGNSDCAAYNDF
                     RELIARKDIDAVQITAPDHWHPLIAIEAARHGKHMYQEKPMGWSVRAARAVERAVRDN
                     GVVFQFGTQQRSDGRFRLACELVRNGRIGRLQTILVGVPGSVSSCPIQPVEPVPKELD
                     YDLWLGPAPMAPYCYQRCRPYTQKDGWSVWYGISDYCMGMIGNWGVHHLDIAQWANAS
                     ELSGPVEIE"
     gene            1139..3667
                     /locus_tag="PKK20_03240"
     CDS             1139..3667
                     /locus_tag="PKK20_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU98934.1"
                     /translation="MLNGVQAFADQLAGLTVLNQVVVPDLWQQEAVSALRAGCDVVVQ
                     APTGSGKTLVFEIWSKQGHNRGQAIYTVPTRALANDKLAEWRARGWNVGIATGDLAEN
                     LDAPVIVATLETQKNRLMQGTGPSLLVVDEYQMIGDADRGLNYELAIAMAPPSTQLLL
                     LSGSVSNPQDVVKWLRRLGRNAHLVRHEHRPVPLEEVHVQHLNYHLPSEIRGYWPRAV
                     AKALADNLGPVLVFAPRRQAAEALAADLARNLPCPDPLQLSVAQRQIVGDHLARLLKS
                     RVAFHHSGLSYGARAGVIEPLTKAGQLRAVVATMGLAAGVNFSLRSVALAGESYRRDE
                     LEHPLKPDEILQMFGRAGRRGIDETGFVLVTANEIRLLDAHTGHLARNGMVDWSALLG
                     IMRAAAQQGRSPFLEAVRVQQRLFTTKPIFLGVEMSLEHPHAPCGLHTDAERARHVRR
                     LIREMWNSQGEWQRFPPPRHVPLEEIKVAPTTAESAPSANPLTPPPALRPVLSEPAAL
                     AKIGSGNLCVVDVQSGLPVYGRALTVADRLSDDRVLIAKWVRRLTQWKGRQAPYTVWK
                     EHIVPRLERGLIAQNTPAVRFVESTRKIVAEVSLGRLTLKVPVDAHGVALWKPAEREV
                     LAPDCARCDLVQRCRELTAATGTALLWRRLGLVDAAGVPTRRGEIVSCFSQGDGLAIA
                     AALEDENYPLDELVYDLANLDAGFRFCGEDNRWAGRLAIACRRAYGIQTAPGYLENGV
                     PPKYGVGAEAVVASVHRNPQAKHAWVSEWLGSGDIDRIIIEWRSLLRQIAHARDIDWP
                     RWKALQACARALLEETRSPTLTDLPPLEYHQTRRIEHRLVLRRH"
     gene            complement(4174..4659)
                     /locus_tag="PKK20_03245"
     CDS             complement(4174..4659)
                     /locus_tag="PKK20_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98935.1"
                     /translation="MGTIFDLQEADARRLFPAPAPRRSGEFKAVRQRRWVARRCRRLW
                     IALDTRIVPEALATDLCHQVGRWLEVELPRSWRVRLAVKADVVYYHNRRFRQGVRRKG
                     RAGVRYLRVFMQHWLAGLLERRWPDWRERLPSDFARGAELPVCEPARGEQSPRDTPAS
                     C"
     gene            complement(4714..5964)
                     /locus_tag="PKK20_03250"
     CDS             complement(4714..5964)
                     /locus_tag="PKK20_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNU98936.1"
                     /translation="MDLSLVFLEKGLELSNQAGRVGLICTSQWLTADYGQNLRRMLSG
                     GRLHEIVDFGSLPVFANASTYPAILVLSPTPASALTVRRINNAEDLNTHGIHGAPAVT
                     VRLSSLTAAPWNLGRLDIARILQRSKLAWKPLSAFGGAYIGCKTGMNQAFVLSSGEAR
                     KLRLEKAALLSYALRGGEVGRYARVEPDAVVIYPYREGPNGSPELIPETELKRDYPHV
                     HAYLLSFKDQLRQRQDSRRFYAKGVEWYRHLRPGTFRYIRPPKLVFKAIAKSACGGFL
                     AENTAFDGANCPAIIPENLEGHDIKFLLGLLNSRLASYHLRSVCPPKLAGYLKFSATC
                     LSETPIRVLDLSKPGDRSHHERMVQLVEQMLELNQRLPAVRTPQEKTALERQIAATDT
                     QIDRLVYDLYGLTEEEIGIVEGAK"
     gene            complement(6240..7895)
                     /locus_tag="PKK20_03255"
                     /pseudo
     CDS             complement(6240..7895)
                     /locus_tag="PKK20_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(8102..12745)
                     /locus_tag="PKK20_03260"
     CDS             complement(8102..12745)
                     /locus_tag="PKK20_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNU98937.1"
                     /translation="MSPDSYVPRTLGHELKSRGRLPSEECLQIGITLADAVEHLHRNR
                     LVHRDIKPSNVIFVNGRPKLADIGLVAEIGATKSFVGTEGFVAPEGPGTPRSDIFSFG
                     KLLYEMATGKDRGDFPAPLTNLAALPDQARLSELNEVILKACEPDPGQRYASMAALRA
                     ELLLVQAGESLRRTRVLERQFRCFRRAAVLALVALLIGILGFLPIQREREARLRAERE
                     RMAWLTQFEAQNVEQLFANDRPREAIASLAGLLREDAANQVAAERLLHALTDRSFALP
                     LTPALEHTAGVAIVRWSADGERVVTASQDGSARVWDGRTGQPVTPPLRHRGPVRWVEF
                     SPEGGLVVTASDDGTARVWDVATASASASIAESRVPGGPDSSEPGLAAWPAEAATGPQ
                     ASAFGDPERRRQLVPPSLAARQMLVLEHQGPVVMANFSPDGLRVVTASTDATARLWDT
                     RTGEPIGAVLGHNDRVNAARFSPDGHWVVTVSDDGTARVWDAELGEPVGGALTHPGGV
                     AWAEFSPDGVQLVTAGRDGVARIWVRHAGEDVSILPEQPSHGRQSAPSRVFESEGQRR
                     LTPGAAEFKAQPPLEHQGPVVMASFSPDGLRVVTASDDGTARVWDARNGRPVTGPLKC
                     GDQVRAARFSPDGLRVVTASADQTARVWDAFDGEAWTEPFAHGHRLTWAEFSPDGTRV
                     ATSSEDGSACVWDVRLGRALRRRVQLGQAVEEALPSADGRWYLVGLLPDRGTPDCAVD
                     VRRSDEEADVPLSVRFPGVASHFRYSPDEQRALAVTDDGLLRIWAVASGKLVDEPLPV
                     GGRVHTVEFSPDSKWIVTGGTDCLAQLWEAARILETPDLVRSGQVQPSSLPYFKDSPE
                     RGAGGEWGITGVAHEAEDLDGSKRRLARVRRVGGGLPHDGEVWSARFSSTANKLVTAS
                     GDGVTRLWSVPEGRLLRELRGHQGAVYHAEFSQDGRRVVTAGDDLTARIWDAETGQAL
                     GQPRWHERSVRFGCFNREGTRLLTLTEGDAAWLWDLTKPRGRGNPLPLRDRIVAAEFS
                     PDGRCVLARSSAGAQAARLWDVMTGRPLSEPLLHAGPVLCAHFTADGTRVLTGAKDGT
                     ITWWEVMRLPSPAPAWLPELASLLVGERTDADEAAHQPGAHRFKGVRAAIAATAASDV
                     YGQWGRWFLADRRERFAAPSGEKTVADYVARQMLTRQVAPLLDVLRLQPTNAVALSRL
                     AATARRGTAGTNALLLSAVSNQVARLAEWNPRDRVALWAAIDNWAQAGRWDDGLATMD
                     RVLSREPETINGWIGRGVLLDWSGRQEEARLAYVRALECADRSASTPDVLRQTMLLNR
                     AALSKRLGRQTEAVADYPSPWNIPPRDTALEARAIDLSAFYNAAFDEAWYDWRASGNR
                     SLLSAWNAVQTGGLGWPREFVSGTPFDPRGLIQAGNPILDRDAPGFPREVRGMPIGQR
                     ATRLCFIHAAHGVAPDGTLLGRYVAHFGDGGQCEIPIRYGEDVRAWRIRDDPAEITRT
                     DWEQKTEPNPADEVVRLFSTWWANPRPEVEIMSLDLVSEGAAAAPFLVAITSR"
BASE COUNT         2303 a         4356 c         3890 g         2211 t
ORIGIN      
        1 cttcgatttc cactgggccg ctcagttcgc tggcattggc ccattgcgcg atatccaaat
       61 gatggacgcc ccagttgccg atcatcccca tgcagtagtc cgagatgccg taccagacgc
      121 tccacccgtc cttctgcgtg tagggacggc aacgctggta acagtagggc gccatcgggg
      181 ccggacccag ccagagatcg tagtcgagtt ccttcggcac aggctcgacc ggttggatgg
      241 ggcaggacga cacgctgcca gggactccca ccaggatggt ctgcaatcgg ccgatccggc
      301 cgttgcgcac cagctcgcac gccaatcgga aacggccatc ggatcgttgt tgcgtgccga
      361 actggaacac cactccgttg tcgcgcacgg cccgttcgac ggcacgcgcc gcgcggacac
      421 tccagcccat cggcttttcc tggtacatgt gcttgccgtg tcgggcggcc tcgatagcga
      481 tgagcggatg ccagtgatcg ggagccgtga tctgcacggc gtcgatatcc ttccgcgcga
      541 tgagttcgcg aaagtcattg taggccgcac aatcggagtt cccgtagaaa gtgtcgacga
      601 cctgcttacc ggcggcgcgc tgggtcgcat gcacgtcgca cacggcaacc accttcacct
      661 ccggaatccc gagaaatgcc cgcatgtcgc cgatgccctg gatgccaagt ccgatgacac
      721 ccagggtcac gcggtcggag ggagcggtcg aacccgtccc gcccaacaca tcggcggggg
      781 tcaattgcca ggtggtggcc gcgccggcta cagcggttgc tttgaggaat tggcggcggg
      841 acaagacgca tttcataggt caaatcagtt tgtcggggcg caccgtacca tcccaaccaa
      901 cttcacacag ccaaaatctt ccggagcgcg caaaccgacc gcgccaattc atgaagtcga
      961 aaaggtcgga gtggggacgg ctcgaccgcc gccatgtcaa gtgaagacgg cgtgcgttac
     1021 cgctccactt gatgaattgg ccctgacaag ccgaagactc tcgaaggcgt gcgaatgcgg
     1081 gctgacaccg aacaggtgaa tttgaggatt ctgcctgtca atggcgcttt cccgatctat
     1141 gctcaacggc gtgcaggcgt ttgcggatca actggccggc ttgaccgtcc tgaaccaggt
     1201 ggtcgtgccc gacctctggc aacaggaagc ggtcagcgct ctgcgtgcgg gttgtgacgt
     1261 cgtcgtccag gcgccgacgg gttcgggcaa aacattggtt ttcgagatat ggtccaagca
     1321 gggccacaat cgtggccagg cgatctacac cgttccgaca cgcgcgctgg cgaacgacaa
     1381 actggccgag tggcgggcgc gcgggtggaa tgtcggcatc gccacgggcg atctggcgga
     1441 gaatcttgac gcgccggtca ttgtcgccac gctcgaaaca cagaagaacc gcctgatgca
     1501 gggcaccggc ccaagcctgc tcgtcgtcga cgaataccag atgatcgggg acgccgaccg
     1561 cgggctgaac tacgagttgg ccatcgccat ggctccgccc tccacacagc tcttgctgct
     1621 gagcggaagc gtcagcaatc cccaagacgt ggtcaagtgg ctgcggcgtc tgggacgcaa
     1681 cgcccacctg gtgcgccacg agcaccgccc cgttccactc gaagaagtcc atgtccagca
     1741 cctcaattac catctgccat cggagattcg aggctactgg ccacgcgcgg tggcgaaggc
     1801 gctcgccgac aacctgggcc ccgtgctggt cttcgccccg cgccgtcagg cggcggaagc
     1861 cctcgccgct gacctggccc gcaacctgcc ttgcccggac ccgctgcaac tgagcgtcgc
     1921 ccagcgccag atcgtgggcg atcacctcgc ccgcctgctc aagtcccgcg tcgccttcca
     1981 ccacagcggc ttgagctacg gggcgcgcgc cggagtgatc gagcccctga ccaaggcggg
     2041 acagcttcgc gcggtcgtcg ccaccatggg cctggccgcg ggcgtcaatt tctccctccg
     2101 cagcgtcgca ctggccggcg aatcgtaccg acgcgatgaa ctcgagcacc cgctcaagcc
     2161 tgatgaaatc ctgcagatgt tcggacgcgc cgggcgacgg ggcatcgacg agacgggctt
     2221 cgtgctggtg acagcgaacg agattcgcct gctcgacgcc cacaccggac atctggcccg
     2281 gaacgggatg gtggattgga gcgcgctctt gggcatcatg cgcgcggcag cccaacaggg
     2341 acgatctccg ttcctcgaag ccgtccgggt ccagcaacgc ctcttcacga ccaaaccgat
     2401 cttcctaggt gtggaaatgt cgctcgaaca tccccacgcg ccctgcggcc tgcataccga
     2461 cgccgaacgc gcccgccatg tccggagatt gatccgtgag atgtggaaca gtcagggcga
     2521 atggcaacgg tttcccccgc cccgacatgt cccgctcgag gaaatcaagg tcgcaccgac
     2581 caccgccgag tcggcaccct ccgccaaccc gctgaccccg ccgccggcct tgcgacccgt
     2641 cttgagcgag cccgcggcgc tcgccaaaat cggctcgggc aacctctgcg ttgtcgacgt
     2701 gcagagtgga ctcccggtct acgggcgcgc cctcaccgtc gcggatcgtc tgagcgacga
     2761 ccgcgtgttg atcgccaaat gggtgcgccg attgacccaa tggaaagggc gccaggcccc
     2821 ttacaccgtc tggaaagaac acattgtccc caggctcgaa cgcggattga tcgcccagaa
     2881 cacccccgcc gtcagattcg tcgaatccac acgcaagatc gtcgccgagg tgagcctggg
     2941 gcggctcacg ttgaaagtgc ccgtcgacgc ccacggcgtc gcgctctgga aaccggccga
     3001 acgcgaggtg ctcgccccgg actgcgcgcg ttgcgatctg gtgcagaggt gccgcgagct
     3061 gaccgcggcc accggcacgg ccctgttgtg gcggcgtctc ggcctggtgg atgccgccgg
     3121 cgtcccgacc cgccgcggcg agatcgtcag ttgcttctcg caaggcgacg gcctggccat
     3181 agccgccgcg ctcgaagacg agaactatcc tctcgacgaa ctggtctatg atctggcgaa
     3241 tctcgacgca ggattccggt tctgcggcga ggacaaccgg tgggccgggc ggctggccat
     3301 tgcgtgccgg cgcgcctacg ggatacagac cgcgcccggc tatctcgaga acggtgtgcc
     3361 gccgaagtat ggtgtcggcg ccgaggccgt ggtggccagt gtgcatcgca acccacaggc
     3421 taaacacgcc tgggtcagcg agtggctcgg ctccggcgat atcgaccgca tcatcatcga
     3481 atggcgcagc ctcctccggc agatcgccca cgcccgcgat atcgactggc cccgttggaa
     3541 agcgctccag gcgtgcgccc gcgccctgct cgaagagacc cgctcgccca cactcaccga
     3601 tctgccaccc ctcgaatacc accagacccg ccgcatcgag catcgcctcg tcctccgccg
     3661 tcactgaccc gcattgccac gggtgttgct ggtcaaggat tccaatcaag ctccgcctca
     3721 aaccgaccag tggttccgac actcggcggt ccttgcgcgt atctggccgg tttgaggcgg
     3781 agctctattc gatgtgcttc ccccgcgcaa gcggcccgtc ttccataacc agcgctttga
     3841 cggcgcaggc aggtgaattg ctggctgcga acgttcctga cttgctgctt ccgtaagttc
     3901 ggacgtctaa gcccggatat ttcataaggc ttcgcggagt ctcatatccg ggctctggca
     3961 aaaccccgcc cggaaaggga atccagagct gcctgtcagt tctgaaagct tcctggcatg
     4021 aaaacagcgt gttcattttt tctgcccctc ggacctggcg cggttttgct tctctagccc
     4081 gcatagcagt cctcttgcga agtctgagtg ccctgttcac cataggggtg tgaaatatgc
     4141 gggctaagag catgtccgac ctcggtgtgg agatcagcaa gaggctgggg tgtcccgagg
     4201 cgactgttca cccctggcgg gttcgcacac cggcagctcc gcgccccgag caaagtcgga
     4261 cgggaggcgt tcgcgccagt ccggccagcg gcgctcgagg agccccgcca gccagtgctg
     4321 cataaaaacg cgcaggtaac ggaccccggc ccggcctttg cggcggaccc cctgacggaa
     4381 gcggcgattg tggtagtaca ccacatccgc cttcaccgcc agtcggacgc gccacgagcg
     4441 cggcaactcc acttcgagcc accggccgac ctggtggcac aggtccgtcg cgagcgcctc
     4501 cggcacaatg cgggtgtcga gcgcgatcca aagtcgtcgg cagcgacggg caacccaccg
     4561 cctctgccgc acggccttga attcgccgga tcggcggggc gccggtgccg gaaacaaccg
     4621 ccgggcatcg gcctcttgca gatcaaaaat cgttcccata acgccgccag cctacgcgca
     4681 cctctgggtc aaatacagtc ccacccccaa caattatttt gcgccctcga caatgccgat
     4741 ctcctcttcg gtcaaaccgt agaggtcata gaccaagcgg tcgatctgcg tgtccgtcgc
     4801 ggcaatttgc cgttcgagcg cggtcttctc ctgcggtgtt cgaacggcgg ggagccgctg
     4861 gtttaactcg agcatctgct cgaccagttg caccatccgc tcgtgatgcg atcggtcgcc
     4921 gggtttcgac aagtcgagga cgcggatggg agtctcgctc aggcaggtgg cgctgaactt
     4981 caagtagccc gcgagcttcg gtggacagac gcttctcaag tggtaagacg ccagccgcga
     5041 attgagaaga cccagcagga acttgatgtc gtggccctcc aagttctcgg gaatgatggc
     5101 cgggcaattc gcaccgtcga aagccgtgtt ctcggccagg aagcctccgc aagcactctt
     5161 tgcgatggcc ttgaatacca gtttcggggg gcgtatgtac cggaatgtgc cgggccgcaa
     5221 atgccgatac cactccaccc cttttgcata gaatcggcgg ctgtcttgtc tctgcctcag
     5281 ttggtctttg aaggaaagga ggtacgcgtg gacatgtggg taatcccgct tcaactctgt
     5341 ctcgggaatc agttccggac tgccattcgg accttcacgg tagggataga tgaccaccgc
     5401 atcaggctca accctggcat agcgcccgac ttctccccca cgcaacgcgt acgaaagcag
     5461 cgcagccttc tccagccgca gtttcctagc ttccccgctg gatagcacaa aagcctggtt
     5521 cattcccgtc ttgcatccga tgtaagcacc accaaacgcg ctcagcggct tccaggccag
     5581 ctttgagcgc tgcagaatcc tggcgatgtc cagtcgtccg aggttccagg gcgccgcggt
     5641 gagagaagat aaccggactg tgacggcggg tgcgccgtga ataccgtgcg tgttcagatc
     5701 ttcagcattg ttgattcgcc tcacggtgag cgcggaagcg ggcgtcggac tcaggaccag
     5761 tatggccgga tacgtgcttg cgttggcaaa aaccggcagg ctcccaaagt ccacgatttc
     5821 atgaagcctg ccgcctgaga gcatgcgccg gagattctgg ccatagtcgg cggtcagcca
     5881 ttgggaagta cagattaaac ctacgcggcc ggcctgattc gacaactcga gtcctttctc
     5941 aagaaagacg agcgaaaggt ccatcttgct ggtcggcgac ttgtagtgca gaacaagtag
     6001 tcagattcgg catgagaaat ccgctgaatg cgcacatacg gcggattccc aatgaccgca
     6061 tcgaagccca ctttcttttc ctgctcgatc ggccctgggg gctgtgcagc cttgacacgt
     6121 ctccccttcc ggtggctcac cttgctgtag ttcccatgca acggtatccc gggcagcgtg
     6181 tagtccagcg gcgcggcggc ggcgggttct cggagttccc cagcggtgtg cagtgtgcgg
     6241 aagacctgcg gaaactcggc gtgccaatcg aaaacgttga tgcggtaacg ctcctcctca
     6301 tccaggagcc caagttgctc ttgttggtaa aaatccggcc cgatcagcgc gttgccgcat
     6361 ttgaggttgt cccccaggtc cggcaacgcg cgctcgcgaa agaggcgaaa gatcgtctgg
     6421 agggtctggc cggtttcgcc ctccaaaacc ttgagcagca gggagagctt ggtgacctcg
     6481 accgcctgcg cgtcaatgtc gacgccaaaa atgtggttca ggaggatgcg cttgcgctcg
     6541 gcgatggtga gcacccaacc cttgcccgtc tgcaccaacg cgggctggct gccccgggac
     6601 cacttggcgg ggtcgtgggc ggtgtaccag tcccgatgcc agtccagaag gaattgatac
     6661 gcgccaatca ggaaggagcc ggacccgcag gcagggtcga gaatgcgcaa ggctgacacc
     6721 tgtttgggcg tcctgccctc gaccagcttc cccaccgtct gccccacgat gtagtccacc
     6781 acgtaggtgg gcgtgtagta aacgccaccg gccttgcgaa cctccggctt ctcctcgacc
     6841 gccgcgcggt ggccctcggt caggcggatg accttgccca ggaactgctc gtagacctgg
     6901 ccgagaatgt cggcggaaat gacatcgaag gcatagggac tgtcggggta gtagagcccc
     6961 cgcagcaaat cgcgcaggag cttgtcgtcg atatcgaggg ccagcgtcag ttcatcgggc
     7021 ggctcgtggc ggcccggttc cgattggaag tggaacaggc cggagttgta gcgcgcatcg
     7081 gcggcctgga agagttcggc caggcgcggg tagatgcggt ccccgttcac gagcgcttgc
     7141 agccggccat gatcctcgat gccgcgcgct tcgcagatgc gcaggaagat gatgcgatca
     7201 atgatgcgct gaacggcaaa gttgagttcg cgctgggtca acttgggatt gcggagcgcg
     7261 aggttgcgcg ccaagtccag ccgccaccct tcgatggtgg cgagaaagtc cgcgtcgaat
     7321 tccgccgtgc cgcgcttgga tttgctggac tcggcaaacc ggtcgaacgc ccctttcaac
     7381 accgcgtcgc ggctgaagat ggacgcaatc cactcccact tctccgcgta gtcgcggaag
     7441 gtgcagaaga aaacgcgggc ggtcgcggcg ggatcatcct tgtgcggctt gaagcgtccg
     7501 tcatagacgg cgaactcctc gaaatcggac aggatgttca gcgaaagttt ggccgtgtag
     7561 gcgtagcggc gcagttgaaa ggcgggcgcc ggctcgtcct tgaccgcgac cgacggcttc
     7621 ttggcttcga ggaagaactt gcgcgtgccc ccgacccgga agcagtagtc cggggctttg
     7681 gtggcctcgc cgacccggag ttggtcttcg tgaacaacgt ccttgtaggc ctcggccagg
     7741 ccctgctcat tgtcgatgtc ccagcccagc gccttgaaga aagggtcgag gaattcgcgg
     7801 cgcagttgcg tctcgttgta ctcgccggaa cggtaggctt cgaggttccg ctcgaagcgg
     7861 gcaacgagtt cgaccacggc ggctggaata ctcatggcaa cagggcgaca gattgcgatc
     7921 gcaattccgt gctgtcaacc gacgggcgga atcctgtcgg gcggaatccc aactcatcct
     7981 ggctgcttcc tggcccagct tgtgcgcggc aaggcgccac aattcgtcat acacctgcgg
     8041 aaggaacgca tccgcagctt gagaaccgga cggccgtgtt gtcgcagcac ctcccacgcc
     8101 ctcatctgga cgtgatcgcg accaggaagg gcgcggccgc cgccccctcc gacaccaaat
     8161 cgaggctcat gatctcgact tccggcctcg ggttcgccca ccacgtgctg aacagccgca
     8221 ccacctcatc cgccgggttc ggctcggtct tttgctccca gtcggttcgt gtgatttccg
     8281 ccgggtcatc gcggattcgc cacgcccgca catcttcgcc ataccggatc ggaatctcgc
     8341 actgcccgcc atcgccaaag tgcgccacat aacggcccag cagcgtgccg tcaggagcaa
     8401 caccgtgcgc ggcgtgaatg aagcagagtc gagttgcccg ctgcccgatt ggcattccgc
     8461 gaacctcgcg cgggaagccg ggcgcgtccc gatccaggat cggattgccc gcctggatga
     8521 ggccacgcgg atcgaacggc gttccactca cgaattccct cggccacccc aaaccacccg
     8581 tctgcaccgc gttccaggct gacagcaaac tccggttgcc gctcgcccgc cagtcatacc
     8641 acgcctcgtc gaacgccgca ttgtagaagg cggagaggtc gatcgcccgc gcttcgagtg
     8701 ctgtgtcccg gggcgggatg ttccacggac tcgggtaatc ggccaccgcc tccgtttgac
     8761 gtcccaaccg tttcgacaac gcggcccggt tgagcagcat tgtctgccgc aacacgtcag
     8821 gagttgaagc ggaacggtcc gcgcattcca gggccctgac gtaggccaga cgggcttcct
     8881 cttgccgccc actccaatcc agcagaacgc cacgcccaat ccaaccgttg atcgtctccg
     8941 gctccctcga cagaacgcgg tccatcgtcg ccaatccatc atcccaccgg cctgcttggg
     9001 cccagttgtc gatcgccgcc cataaggcca cgcggtcccg cggattccat tccgccaacc
     9061 gggccacctg gttgctcacc gcgctcagca acaaggcatt cgttcccgcc gttccgcgcc
     9121 gggccgttgc cgccagacgc gacaaagcga cggcgttcgt gggctgcaac cgcagcacgt
     9181 cgagcagcgg cgccacctgc cgcgtcaaca tctggcgcgc gacataatcg gccaccgtct
     9241 tttccccgga cggcgccgca aaccgctccc gtcggtcagc cagaaaccat cggccccact
     9301 gcccgtacac gtcgctggct gccgtcgccg cgatcgcggc acgaacgccc ttgaaccggt
     9361 gcgcgcccgg ttggtgcgcc gcttcatccg cgtcggtccg ttcccccaca agaagactcg
     9421 ccaattcggg cagccaagcc ggcgccggac tcggcagccg catcacttcc caccaggtga
     9481 tggtgccgtc cttcgccccc gtgagcacgc gcgtcccgtc cgcggtgaaa tgagcgcaga
     9541 gcaccggccc ggcgtgcaac aggggttcgc tcagcggacg gcctgtcatc acgtcccaga
     9601 gtcgagccgc ctgtgctcca gcgctcgagc gggccaacac gcagcggcca tccgggctga
     9661 actcggcggc gacgatccga tcgcgcaacg gcagaggatt gccccggcct cggggcttgg
     9721 tcaggtccca aagccaggcc gcgtctccct ccgtcaacgt caacagccgt gttccctccc
     9781 gattgaaaca accaaaccgt acgcttcgct cgtgccaccg cggttggccc agggcctggc
     9841 cggtttcggc atcccagatg cgcgcggtta ggtcgtcccc cgccgtcacg acgcggcgtc
     9901 cgtcctgact gaactcggca tgatacaccg ccccttgatg cccacgcagt tcccgcagca
     9961 gccggccctc gggcacggac cataggcgcg tgaccccatc gcccgacgca gtgaccagct
    10021 tgttcgccgt ggaactgaat cttgccgacc atacctcccc atcatggggc aggccaccgc
    10081 ccacacgcct cactcgagcg aggcgcctct tagacccgtc caagtcctcg gcttcatgcg
    10141 caacaccagt gattccccat tcccctcccg cgcctcgctc aggggaatcc ttgaaatacg
    10201 ggagggagga gggctgaact tgacctgatc gaaccaggtc cggggtttca aggattctgg
    10261 cggcctccca aagctgggcc aggcaatcgg ttccccccgt cacgatccac ttgctatctg
    10321 gactgaactc gactgtgtgg acccgcccgc ccaccggcag cggttcgtcc accaacttgc
    10381 ccgaggctac agcccagatg cgcaacaatc cgtcatccgt caccgccagc gcgcgctgtt
    10441 cgtccgggct gtagcggaag tgcgacgcca cgccggggaa ccggaccgat agcggaacgt
    10501 ccgcctcctc gtcggagcgg cggacatcta cggcgcagtc aggcgtgccg cgatcgggca
    10561 agaggccgac gagataccaa cgaccatccg cgctgggcag ggcctcttcg acggcctgcc
    10621 cgagttggac acgtcggcgc agagcccgcc ccagccgcac atcccaaaca caagcgcttc
    10681 catcctccga actcgttgcc acccgcgtgc cgtccggact gaactccgcc caggtcagcc
    10741 gatgcccatg cgcgaacggc tcggtccagg cctcgccatc aaaggcatcc cagacccggg
    10801 ccgtctggtc cgccgaggcc gtcaccacgc ggagtccatc gggactgaac cgcgccgcgc
    10861 ggacttggtc gccgcacttc aacggccccg tcaccggacg gccattgcgg gcatcccaca
    10921 cgcgcgccgt gccatcgtcc gaggccgtca ccacgcgcag gccgtccgga ctgaagctgg
    10981 ccatgaccac cggcccttgg tgttcgagag gcggctgggc cttgaactcc gcagcccccg
    11041 gcgtcagccg gcgctggccc tcgctttcga agacccgaga cggcgcggac tgacgtccgt
    11101 ggctaggttg ctcgggaagg attgacacat cctctcctgc gtgccgcacc caaatccgcg
    11161 ccacgccatc gcgcccggcg gtgaccaact gcacgccatc ggggctgaat tcggcccatg
    11221 ccaccccgcc tgggtgcgtc aatgcgccgc cgaccggttc ccccagttcc gcatcccaga
    11281 cccgcgcggt cccgtcgtcg gagacggtca ccacccaatg cccatcggga ctgaaccgag
    11341 ccgcgttcac gcggtcgttg tggcccaaaa ccgcgccgat tggttcaccg gtccgcgtgt
    11401 cccagaggcg ggccgtggca tccgtggaag cggtgacgac gcgcagtcca tcgggactga
    11461 agttcgccat cacgaccggt ccttggtgtt cgaggaccaa catctggcga gccgcaaggc
    11521 tcggagggac gagctgccgc ctccgctcag gatcgccaaa tgcggatgct tgaggcccgg
    11581 ttgcagcttc ggcgggccaa gccgcgagcc ccggttcact cgaatcaggg cctccgggaa
    11641 ctcggctttc cgcgatcgac gcgctcgccg aagctgtggc cacgtcccac acccgtgccg
    11701 ttccgtcgtc cgaagccgta accaccagcc ctccctccgg actgaattcc acccagcgaa
    11761 caggtccgcg atgccggagc ggcggcgtga cgggctggcc ggtccgtccg tcccatacac
    11821 gtgcgctgcc gtcctgcgag gctgtcacga cccgctcgcc atcggcgctc caacgaacga
    11881 tcgccacgcc ggcggtgtgc tcgagcgcgg gggtgagcgg cagggcaaat gaccgatccg
    11941 tgagcgcgtg gagcagccgt tcggccgcga cttggttcgc cgcatcttcc cgcagcaatc
    12001 ccgccagact cgcgatcgct tcgcggggac gatcattggc gaacagttgc tcgacgttct
    12061 gggcctcgaa ctgggtcaac cacgccatgc gttcgcgctc ggcgcgcagc cgcgcctcgc
    12121 gctcacgctg tatcggcaga aagccgagga tccctatcag taacgccacc aatgccagga
    12181 ccgccgcacg gcggaaacac cggaattgcc gctcgagcac gcgcgtcctc cggagcgatt
    12241 cccccgcctg caccagcaac aactcggctc gcaaggccgc catgctcgcg taccgttgcc
    12301 ccggatccgg ctcgcacgcc ttgaggatga cctcgttcag ttccgacagg cgcgcctggt
    12361 ccggcaatgc cgccagattc gtcaacgggg ccgggaagtc gccccggtcc ttgccggtgg
    12421 ccatttcgta gagcaacttg ccgaagctga agatgtccga ccggggtgtc cccggacctt
    12481 ccggcgccac aaacccctcc gtcccgacga acgatttcgt cgcgcctatc tctgcaacaa
    12541 gaccaatgtc cgccagcttg gggcgtccat tcacgaaaat gacgttcgac ggcttgatgt
    12601 cgcgatggac cagccggttg cgatgcagat gctcgacggc gtcggccagt gtgatcccga
    12661 tctgcaggca ctcttccgac ggcagccgcc cccgcgactt cagctcgtgg cccagggtgc
    12721 gaggcacgta ggagtccgga gacatcgagc aggtggcagg
//
LOCUS       DAOQRO010000052        12753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4027_length_12753_cov_4.586077, whole genome shotgun sequence.
ACCESSION   DAOQRO010000052 DAOQRO010000000
VERSION     DAOQRO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12753
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_12753_cov_4.586077"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1021
                     /gene="ilvE"
                     /locus_tag="PKK20_03265"
     CDS             128..1021
                     /gene="ilvE"
                     /locus_tag="PKK20_03265"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNU98938.1"
                     /translation="MTIYIDGRYCSERDAKISVFDHGLLYGDGVFEGIRAYHGRIFKL
                     KEHIDRLFCSAKAILLRIPMTHAQVMSAVVETCRRNRIRDGYIRLVVTRGVGTLGLNP
                     ERCRHPSVIIIADRIQLYPPEFYEKGLAIVTVPTTRNLNNAVSPAIKSLNYLNNILAK
                     IEANIAGVEEAIMLNATGYVAECTGDNVFIVKAGQLLTPPLYAGALYGITRGVVIELA
                     RECGVPVSETNLTRYDLFNADECFVTGTGAEIVPVVKIDGRIIADGKPGTLTRDLEKR
                     YRALTQISGEPIAPARKRRAI"
     gene            1069..1605
                     /locus_tag="PKK20_03270"
     CDS             1069..1605
                     /locus_tag="PKK20_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNU98939.1"
                     /translation="MLCMVCKQQEATVHLTQVVEGKIKKLDLCEACSKAKGVDDPTGF
                     SLADLLMGLGASQEIAQAASSGESSCPTCGFTQADFKKSGRLGCPDCYRAFSDGLEAL
                     LKTMHKGHQHVGKAPGSLAGQTQTQSRTPGRSPEDRLQALQRSLEEAIKVENYEEAAR
                     IRDEIKALKVPNGGAVTA"
     gene            1611..2720
                     /locus_tag="PKK20_03275"
     CDS             1611..2720
                     /locus_tag="PKK20_03275"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HNU98940.1"
                     /translation="MHLLEFLRPPQEAAKQSGPQDKIVLSSRVRLARNLSGSPFPGWA
                     KKPERIRACEAIRTAVQELPEMQGAFAESMDALSGLDKQILVERHLVSREHAAKGTGS
                     GLVLSKDESLCVMINEEDHLRMQSLKPGLQLHEAWQTIDTLDTRLENRLTYAFSPELG
                     YLTACPTNVGTGMRVSAMLHLPGLVLAEQINQVVQAVNKLGLAVRGLYGEGTEALGNI
                     FQISNQMTLGETETVIVERLSKVVAQIIEHEENARGTLLEKKPKVVFNHIGRAYGILA
                     NAHSISSKEAMNLLSLLRIGLDLGLFPGTARSLVDELTILTQPAHLQARYSEKLSAEE
                     RDIRRADMMREALRTVNRPVIPPDTAEPLDKPPPA"
     gene            2783..5272
                     /locus_tag="PKK20_03280"
     CDS             2783..5272
                     /locus_tag="PKK20_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNU98941.1"
                     /translation="MSEENLSNFTPRAQQVLALARKEADRFNHNFVGTEHILLGLIKL
                     GQGVAVNVLQKMGLDLDTVRLEVEKQVGTGPDQKMIGNIPYTPRVKKVLSLAGKEAKA
                     LNHTYVGTEHILLGLLREGDGVAARVLKNLEIDIEQTRQEILRELDPNLVSGEEGGEE
                     TAAKGSGRKGDVKTPALKAFGRDLTEIARKGEMDPVIGRQDEIERVIQILCRRTKNNP
                     VLLGEAGVGKTAIVEGLAQEIVRGNVPELLREKRLITLDLALMVAGTKYRGQFEERIK
                     AVMDEIRRSKTVILFIDELHTIVGAGSAEGTMDASNIIKPALSRGELQCIGATTLTEY
                     RKYIEKDAALERRFQTVRVEPPTVDEAILILKGLRPKYEAHHKIDLTDQALEAAVELS
                     DRYITGRFLPDKAIDVMDEAGSRARIGATTRPPDVKQLEADIQEIKAQKERAIKEQDF
                     EGAAAMRDKEKQAKQKLESILAEWKTSREEKRVRVDDEDIMHVVAKWTGIPLKRMGRD
                     EAHKLLDMEKELTKVVIGQSEGVSALCKALRRSRADLKDPKRPIGTFMLLGPTGVGKT
                     LLAKTLAEQMFGDAKSLIQLDMSEYMEKFNVSRLVGSPPGYVGYEEGGQLSEQVRRKP
                     YCVVLFDEIEKAHPDVMNMLLQILEEGKLTDSFGRQVDFRNTVILMTSNVGSEGLRKQ
                     TSIGFTPASEQFTYEKMKEKILDEAKRVFRPEFLNRLSDVIVFRSLTRPDLMQILDLE
                     ISKVMERLKHKNIQIELDSKAKDFLTDKGYDPQYGARPMRRAVERYLEDPLAEEIIRG
                     NLLQGEPIHVTVDQDKLVFAQMAASEAAVSG"
     gene            complement(5273..5719)
                     /locus_tag="PKK20_03285"
     CDS             complement(5273..5719)
                     /locus_tag="PKK20_03285"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNU98942.1"
                     /translation="MNLIPIYEGLADVNRLRIVHLLSQGPLAVKHLQGILGISQVRVS
                     KHLAYLRKRGMVEVDRNRNWMIYRLPALPSAELTAQVKCLAECAPGMPILDADLSARL
                     AIQAEVDRIAASGEGSEPTMEAASPTSSQPAANVGWGLQEEDGYVD"
     gene            complement(5975..7393)
                     /locus_tag="PKK20_03290"
     CDS             complement(5975..7393)
                     /locus_tag="PKK20_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98943.1"
                     /translation="MKTQSNSRITRRRFLSNTTAAAAAVSLAPRHVLGGAAFVAPSEK
                     VNVAIVGTGGQGRTNMRELFRHADAQVIAVADPIERENLDAFYFKGIAGRLPVKAEAE
                     THYGAKSPNFRVTDYEDFRVMLEKEKAIDAVLCATTDHLHASVCVQAMRQGKHVYCEK
                     PLAHNVWEGRLMARIARETGVATQMGNQGHSGEGLRQTYEWIRAGVIGDVGEVHAWTS
                     ASRWSKKYTHGRPPEEPVPQGVNWDLWIGPREMRPYSSAYTPVTWRDYWDFGTAPIGD
                     FFCHNFDAACFALELTAPSSIEAFGAGGVDSEMAPPGGIYYYQFPARGSLPPVKFTWY
                     DGGMRPRFPEGLEEDDQLGAGGNGTLFVGTKGMITCPGWAGDPRLLPGSRMDNFQRPP
                     KTLPRSKGHHRDWLDACKGGPAPSANFTYGAGLNEMGLLGLVAMRVGKKMFWDAAAGK
                     SPNAPEADIYLRGIYRRGWELT"
     gene            complement(7574..10222)
                     /locus_tag="PKK20_03295"
     CDS             complement(7574..10222)
                     /locus_tag="PKK20_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98944.1"
                     /translation="MEFPDGLHYSYRPMRATVHNSRSGSRSDSPPRAPSGRGVSWLLV
                     TLGCLLAVLGFLFAGSFSPEKILFSSDGPLGGTSSEAMSMPGGFRCIWRDLNWVGSWG
                     GNMPPNVSYLWFWLLGPLGFAKFYAPLSVLTVGLSVWVLFRQLRFKPWIGLLGALAAA
                     LNTDFFSYACWGLGTLTICMAWIFVALAALARSTPSSDWLKAAVGGFAVGMAILEGVD
                     SGAILSLYVAAFVLFQAVHVDPSNWRAWVHGIGRIGVVAVCAAVVAFAALSMLVSTQI
                     KGVVGMEQDTRTKAQRWYDATQWSLPKVETLRAVVPGLFGYRLDTPGGGNYWGRVGER
                     PGVLARHSGSGFYAGVVVVLLALWGAANAGRGNAGPFASEERKWIWFWTGAACLSIAF
                     AWGKHAPFYQALYALPYFSTIRNPIKFLHPFSLSLVILSGYGLEALARLYLAKTAAAA
                     RSASVPLKAWWLQARGFERKWGFFSAVALGSALLGWLLYFASRNDLENHLRTAISPDL
                     APAIAGFSLTEVGWSVLFLALTLLVFILIMSGALSGSRASWGAVALGAVMLADLVRAN
                     TPWVYYWNYRERYATNAIIDFLRARPYEQRVAMPPLQLGEAYALLQQIYHAEWLQHQF
                     QYYNVQSLDVIQEPRTSIENARYRGAFAAQHEAGLLRQWQLTNTRYLFGVMGGMADFL
                     NQRLDPTQQRFRVHVPFALVQEREGKPILMQTNTTGPFALIEFTGALPRGKLYSQWQV
                     NTNDDEVLRTLTNPGFDPAESVVISEPAIGLPASGSTNSAPGTAEVLGYAPKRIRLKT
                     HAAIPSVLLWNDKYDPHWSVSVDGQPQPLLRCNYLMRGVCVPNGEHEIEFRFDVPSRP
                     LWISLAAIAVGLALSGFLFCQRLEAD"
     gene            10368..10649
                     /locus_tag="PKK20_03300"
     CDS             10368..10649
                     /locus_tag="PKK20_03300"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNU98945.1"
                     /translation="MNRCRLHIWYTGHVQGVGFRYSVRSVAQGYEVSGLVRNLLDGRV
                     ELVAEGARDELDAFRRGILDSGLSGLIRNEEVVWERAEGNLRGFVISNE"
     gene            10668..11390
                     /locus_tag="PKK20_03305"
     CDS             10668..11390
                     /locus_tag="PKK20_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNU98946.1"
                     /translation="MKFAILADIHANLEALEVVLADAKEQQATHYACLGDVVGYCANP
                     KQCLDIIRGMGMPCIKGNHDEYCSMDEALTGFNPHAAQAVEWTRNQLTENDRKWLRDL
                     KYMRLVASFSIVHATLDGPQRWGYVFDKLAAAASFTYQNTALCFFGHTHVPIAFIRDS
                     VVRGGTYSKFRIEPGKKYFVNVGSVGQSRDGVPRATYVIYDMAEGSIELRRLEYDMAK
                     TQEKIRAAGLPRRLADRLSEGR"
     gene            complement(11482..12669)
                     /locus_tag="PKK20_03310"
     CDS             complement(11482..12669)
                     /locus_tag="PKK20_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98947.1"
                     /translation="MPRPLNVGLVGGGRGAFIAQPHQKAIHFDGTRRVVAGALFPDSK
                     IALEEAAKWPYPITGYGSYDEMIGAQKTLPEDQKLDYILIVTPNHVHFDPAMKAIKAG
                     IPVFCEKPLTVNLKEANALVRAVRARNIPFGVAHTYLGHWTSRLSRYIVRAGLLGEVR
                     WVDSYYLQGWLATKVEATGAQGAVWRTNPKTAGGSGCGGDIGTHALMQLRYVTGLEVA
                     QVSAQLETFVEGRLLDDHFTIYCKLSNGSKALVRATQIAIGHKNDLGIEINGTKGTLR
                     WRQEDPECLTIHLSNQPDRVYWRGAVTPGDGFLPKDVPASLMAEPTIPSGHPEAFHDA
                     FARLHRCFEEDVRKYQAGAAFKCDGAKYANIEDGWTGIAFIDACVKSSKKKGAWTTMP
                     KPI"
BASE COUNT         2590 a         3979 c         3910 g         2274 t
ORIGIN      
        1 ccgggacagg tcattcgcct actcacccgc ccggttcgac tttagagcag ccctgtgtcc
       61 gggctggaga gttcacctaa atgattgcac gttgttcgac cctttcatac gctgcggctt
      121 gctttgtatg actatctata ttgacggccg gtactgcagc gagcgtgatg cgaagatttc
      181 cgtgttcgat cacgggctgc tttacgggga tggcgtgttc gagggcatcc gcgcctatca
      241 cggccgcatc ttcaagctca aggaacatat cgaccggctc ttctgctccg cgaaggcgat
      301 tctgcttcgg attccgatga cgcacgcgca ggtgatgagc gctgtggtcg agacctgccg
      361 ccgcaatcgg attcgggacg gctatatccg gttggtggtg acacggggtg tcgggaccct
      421 cggtctcaac ccggagcggt gccgccatcc gtcggtcatc atcatcgccg ataggattca
      481 gctctatccg cccgagtttt acgaaaaagg cctggcgatc gtcacggtac caaccacgcg
      541 gaacctcaac aacgccgtca gcccggccat caagtcgctc aactatctca acaacatcct
      601 cgccaagatc gaggcgaata tcgccggcgt ggaggaggcg atcatgctca acgccaccgg
      661 ctacgtggcc gaatgcaccg gggacaacgt gttcatcgtc aaggccggtc aattgctcac
      721 gccccccctt tacgcgggag cgctctacgg gatcacgcgc ggcgtggtca tcgaactcgc
      781 cagggaatgc ggcgtcccgg tgtctgaaac caatctgacc cggtacgatc tgttcaacgc
      841 ggacgagtgc tttgtgaccg ggaccggggc cgagatcgtt ccggtggtca agatcgacgg
      901 acgcatcatt gcggacggca aacctggaac gctcacccgg gatctcgaga agcggtatcg
      961 cgccttgacc cagatttccg gggagccgat cgcgccggcc aggaagcggc gggcgatttg
     1021 acattgcgct tggttcggtg ggatgtagct tcttgtattg aattgcgtat gttgtgcatg
     1081 gtgtgcaaac agcaagaggc caccgtccat ctcacccagg tggtcgaagg caagatcaag
     1141 aagctcgatc tttgcgaggc ctgctcgaag gccaaaggcg ttgatgatcc gaccgggttt
     1201 tcgctcgccg atcttctgat gggtttgggc gcgtcgcaag agattgctca agccgccagc
     1261 agtggcgaat cgagttgccc cacatgcggg ttcacgcagg ctgacttcaa gaagtccgga
     1321 cgtctcggtt gcccggattg ttaccgggcg ttcagcgacg gcctcgaggc ccttctgaag
     1381 accatgcaca agggccatca acacgtcggc aaggcccctg gcagcctcgc cggccagacc
     1441 cagacccaga gccgaacgcc cggacgctcg cccgaggacc gcctccaggc cctgcagagg
     1501 agtctcgagg aggcgatcaa ggtcgagaat tacgaagagg ccgccaggat ccgcgacgag
     1561 atcaaagccc tgaaagtccc aaacggcggt gccgtcaccg cctaagccgc atgcacctgc
     1621 tggagtttct ccgccccccc caggaagcag ccaaacagtc cggcccgcag gacaagatcg
     1681 ttctgtccag tcgcgtccgg cttgcccgca acctctccgg cagcccattc ccgggctggg
     1741 ccaaaaagcc ggagcgaatc cgggcttgcg aggccattcg caccgccgtc caggaactgc
     1801 ccgagatgca gggggccttt gccgagtcga tggatgctct ttcggggctg gacaagcaga
     1861 ttctggtcga gcgccatctc gtcagccgcg agcatgcggc caagggcacg ggcagcggcc
     1921 tggtcctcag caaggacgag tcgctctgcg tcatgatcaa cgaggaagat cacctccgca
     1981 tgcagtccct caaaccgggg ctgcaacttc acgaggcctg gcagaccatc gatacgctcg
     2041 ataccaggct cgagaatcgc ctgacgtatg cgttctctcc ggagttgggc tacctcacgg
     2101 cctgcccaac caacgtcgga acgggcatgc gtgtgagcgc gatgctccat ttgcctggct
     2161 tggtcctcgc cgagcagatc aatcaagtgg tccaggccgt caacaagctc ggtttggccg
     2221 ttcgcggtct ctacggcgag ggaaccgagg cgctgggaaa catcttccag atctcgaacc
     2281 agatgactct cggcgaaacg gaaactgtga tcgtcgagcg cctgagcaag gtcgtggccc
     2341 agataatcga acacgaggaa aacgctcgcg ggacattgct cgaaaagaag cccaaggtcg
     2401 tttttaacca catcggccgt gcctacggta tcctcgccaa cgcccacagc atctcgagca
     2461 aggaagcgat gaacctcctc tccttgctcc ggatcgggct cgacctcggg ctcttcccgg
     2521 gcaccgcccg gtcgttggtg gatgagttga ccattctgac tcagccggct catttgcagg
     2581 ccaggtattc cgagaaactc tccgccgaag aacgggacat ccgccgcgcg gacatgatgc
     2641 gggaggccct gcgcacggtc aatcgcccgg tcattccccc ggacactgcg gaaccgctgg
     2701 acaaaccgcc cccagcgtag cattgtgtga cgggtacgcc gagtcgtccc gcgtgaaccg
     2761 cggattcgca gtcgagagca ttatgagtga ggagaacttg agcaatttca cgccccgcgc
     2821 ccagcaggtg ctggccttgg cccgcaaaga ggccgaccgg ttcaaccaca acttcgtggg
     2881 gaccgaacac atccttttgg gcctgatcaa attgggacag ggcgtcgccg tcaacgtgct
     2941 tcagaagatg ggcctggacc tggataccgt ccgactcgaa gtcgaaaaac aggtcggtac
     3001 cggaccggat cagaagatga tcggcaacat cccgtacacg cctcgcgtca agaaagtcct
     3061 ctccctcgca ggcaaggaag ccaaagcgct caaccacacg tacgtcggca ccgaacacat
     3121 ccttctgggc ctcctccgcg agggcgacgg tgtcgccgcc cgcgtgctca agaacctcga
     3181 gatcgacatc gagcagacgc gccaggaaat cctccgagag ctcgatccga acctcgtctc
     3241 gggcgaggag ggcggcgagg aaaccgccgc caagggttcg ggcaggaagg gcgacgtcaa
     3301 gacccccgcc ctcaaggctt ttggacggga cctgaccgag attgcccgca agggcgagat
     3361 ggatcctgtc attggacgcc aggacgagat cgagcgggtg atccagattc tgtgccggcg
     3421 aaccaagaat aacccggtcc tgctgggcga ggccggcgtc gggaagacgg ccattgtcga
     3481 ggggctcgcc caggaaattg tccgcggcaa tgtgcccgag ttgctgcgcg agaaacgcct
     3541 catcaccctc gacctggcac tgatggtcgc gggcaccaag taccgagggc agttcgagga
     3601 gcggatcaag gcggtcatgg acgaaatccg ccgatcgaaa accgtcattc tcttcatcga
     3661 cgagttgcat accatcgtcg gcgccggttc ggccgagggc accatggacg cctcgaacat
     3721 catcaagccc gccctgagtc ggggcgagct gcagtgcatc ggcgccacaa ccctcaccga
     3781 gtaccgcaaa tacatcgaga aagacgccgc cctcgaacgt cgcttccaga ccgtcagggt
     3841 cgagccgccc actgtcgacg aggctatcct catcctcaag ggcctccgtc ccaagtacga
     3901 ggcgcaccac aaaatcgacc tcaccgacca ggcgctcgaa gccgcggtgg aactctccga
     3961 ccgctatatc accggacgat tcctccccga caaggccatc gacgtcatgg acgaagccgg
     4021 ctcgcgcgct cgcatcggcg ccaccacccg cccccctgat gtcaagcagc ttgaagccga
     4081 catccaggag atcaaggccc agaaggagcg cgcgatcaaa gagcaggatt tcgagggcgc
     4141 cgctgccatg cgcgacaagg aaaagcaggc caagcagaag ctcgaatcca tcctcgccga
     4201 gtggaagacc tcgcgcgagg agaaacgggt tcgagtcgac gacgaggaca tcatgcacgt
     4261 cgtcgcgaaa tggaccggga ttccgctcaa acgcatgggg cgcgatgagg cccacaagct
     4321 cctcgatatg gagaaggagt tgacgaaggt cgttattggc cagagcgaag gcgtcagcgc
     4381 cctgtgcaag gccctccgcc gctcgcgcgc cgatctcaag gaccccaaac ggccaatcgg
     4441 cacgttcatg ctcctgggcc cgacgggcgt cgggaagacc ctcctcgcca agactctcgc
     4501 cgaacagatg ttcggcgacg cgaagtcgct tatccaactc gatatgagcg agtacatgga
     4561 gaagttcaac gtctcgcggc tggtcggatc gcctccggga tacgtcggct acgaggaagg
     4621 cggccagctc tccgaacagg tccgccgcaa accctattgc gtcgtcctct tcgacgagat
     4681 cgagaaggcc caccccgacg tcatgaacat gttgctccag attctcgagg aaggaaaact
     4741 caccgacagc ttcggacgcc aggtcgactt ccgaaacacc gtcatcctca tgacctcaaa
     4801 cgtcggctcc gaaggactcc gcaagcagac ctcgatcggg ttcacccccg ccagcgagca
     4861 gttcacctac gaaaagatga aggagaagat cctcgatgag gccaagcgcg tgttccgacc
     4921 ggagttcctc aatcgcctca gcgacgtgat cgtgttccgg tccctgacaa gaccggactt
     4981 gatgcagatt ctcgatctcg aaatctcgaa ggtcatggaa cgtctcaaac acaaaaacat
     5041 ccagatcgaa ctggattcca aagccaaaga cttccttacc gacaagggct acgacccgca
     5101 gtacggggcg cggcccatgc gccgcgccgt cgagcgctac ctcgaagatc cgctcgccga
     5161 ggaaatcatc agaggcaacc tcctccaggg cgaaccgatc cacgtcaccg tcgatcagga
     5221 caagctcgtc tttgcccaaa tggccgcctc cgaagccgcc gtctccggct gatcagtcga
     5281 cgtatccatc ctcctcctgc agcccccatc cgacgttggc cgccggttgg gacgaggtcg
     5341 gggatgccgc ctccatggtc ggctcagacc cttcgccaga agccgcgatc cggtccacct
     5401 cggcttgaat tgccagccgc gccgagagat ccgcatccag aatcggcatg cccggcgcgc
     5461 actcagccag gcatttgact tgtgccgtca actccgcgga cggcaaggct ggaagacgat
     5521 agatcatcca gttccggttc ctgtccacct cgaccatgcc ccgctttctg aggtaggcca
     5581 gatgcttcga cacgcgcact tgcgaaatcc ccagaatacc ttgcagatgc ttgaccgcca
     5641 gcggtccctg gctcagcaga tgaacaatcc tgagccggtt cacatccgca agcccttcgt
     5701 agatcggaat gagattcatg tgcgcaaatg tatgtatgta tgggaatatg cgtcaatagg
     5761 tttgaacttg ccgtcccgct gagcggtcag gttcgccatc ggacacagcg agcgttcgtg
     5821 tcagtgttcg cgatgcggtc acaaccgcgc cacgcaggtc aggaggtcag gattggtttg
     5881 ccgcgttcag ggggtccggg atgctatggc agtggtgcca gtccaggccg ccagcaccaa
     5941 gcgcgaatcc aggctgggat ttgatccgga cgatctaggt caactcccat ccccggcggt
     6001 aaatgccgcg caaatatatg tccgcttccg gggcgttggg tgacttgccc gcggcggcgt
     6061 cccagaacat tttcttgcca acgcgcatcg cgaccaggcc gagcaggccc atctcgttca
     6121 ggcccgcccc atacgtgaaa ttcgcgctcg gggccggacc acccttgcag gcgtcgagcc
     6181 agtcccggtg atggcccttg gatcggggca gcgttttggg agggcgttgg aagttgtcca
     6241 tgcgcgatcc gggcaggagg cgcggatcgc cggcccaccc tgggcatgtg atcatgccct
     6301 tggtgccaac aaacagcgtc ccgttgccgc cggcgcccaa ctgatcgtcc tcttcgagtc
     6361 cttccgggaa ccggggtcgc atcccgccgt cgtaccaggt gaacttgacc ggtggaaggc
     6421 tgccccgagc gggaaactgg tagtagtaga tgccgcctgg aggcgccatc tcggagtcca
     6481 ctccgcccgc gccgaaggcc tcgatgctcg aaggcgccgt caactcgagg gcaaaacagg
     6541 ccgcatcgaa attgtgacag aagaagtccc caatcggcgc tgtcccaaaa tcccagtaat
     6601 cccgccatgt cacgggggta taggccgaac tgtaggggcg catctcgcgc ggaccgatcc
     6661 agagatccca attgacacct tgcggcaccg gttcctcggg cggacgcccg tgcgtgtact
     6721 tcttgctcca gcggctcgcg ctggtccagg catggacctc gccgacgtcg ccgatgactc
     6781 cggcgcgaat ccactcgtaa gtctggcgga ggccttcgcc ggaatgtccc tgattgccca
     6841 tctgggttgc gacaccggtt tcgcgggcga tcctggccat caggcgccct tcccacacat
     6901 tatgagcgag cggtttctcg cagtagacgt gcttgccctg gcgcatggcc tgtacgcaca
     6961 cagaggcgtg gagatgatcc gtggtggcgc aaagaaccgc gtcaatggcc ttctctttct
     7021 cgagcatgac gcggaagtcc tcgtaatccg tcaccctgaa gttaggggat ttggccccgt
     7081 aatgggtctc ggcctccgcc ttgacgggga ggcgaccggc gatgcccttg aagtaaaacg
     7141 catcgagatt ctcccgctcg atcgggtccg ctaccgcgat cacctgcgca tcggcatgcc
     7201 ggaagagttc gcgcatgttc gtgcgcccct ggcctcccgt ccccacgatc gcgacgttga
     7261 ccttctcgct cggcgccaca aacgccgctc ctcccagcac gtgccggggc gccagcgaga
     7321 cggctgcagc cgcagccgtg gtgttcgaga gaaatcgacg tcgggtgatg cgagaattgg
     7381 attgagtctt cataacattg cgcaggcgca gcccgctgtt gccagcggct tggaatcgat
     7441 ggccagagca tgaagtcgga acccgggcga ttcaactgga aaagccgagg ggaagagccg
     7501 agggcgacat caatgatgca gcgccgcgtc gcggcgggac gtccgcggcc atgtgcaaaa
     7561 tcgctcttcc tcttcagtcc gcttccagcc gctgacagaa aaggaatccg gaaagggcca
     7621 agccgacagc gattgccgcc aggctgatcc acaaaggacg agagggcacg tcgaagcgga
     7681 actcgatctc gtgttcccca tttgggacgc agacgccgcg catcagatag ttgcagcgga
     7741 gcagaggctg cggctggcca tcgaccgaaa cggaccaatg gggatcgtac ttgtcattcc
     7801 agagaagcac acttggaatg gccgcatggg tcttgagacg gatcctcttc ggggcgtagc
     7861 cgagcacctc ggcagttccg ggagcgctgt ttgtcgaccc cgaggccggc agcccgatgg
     7921 ccggctcgct gatcaccacg ctctcggcgg ggtcgaatcc gggattcgtc agcgtgcgca
     7981 gcacctcatc gtcgttcgtg ttgacctgcc attgtgaata gagcttgccg cgcggcagcg
     8041 cgcccgtgaa ctcgatgagg gcgaatggcc cggttgtgtt ggtctgcatc agaatgggtt
     8101 tcccctcccg ctcctggacc agggcgaacg ggacatgcac ccgaaatcgt tgctgggtcg
     8161 gatcgagcct ttgattgagg aaatcggcca tgccgcccat gacgccgaag aggtaacggg
     8221 tgttggtgag ttgccattgc cggagcagtc cggcctcgtg ctgcgcggcg aaggcgccgc
     8281 ggtacctggc gttctcgatc gaggtgcgcg gttcctggat cacatcgagc gactgaacgt
     8341 tgtagtattg gaactgatgc tggagccatt cggcgtgata gatctgctgc agaagagcgt
     8401 acgcctcacc gagttgcagg ggcggcatcg ccacccgctg ctcgtaaggc cgggcgcgca
     8461 agaaatcgat gatcgcgttt gtggcgtacc tttcccggta attccagtag tagacccaag
     8521 gtgtgttggc tcgaaccaga tccgccagca tgaccgcccc caaagcgacg gcgccccagg
     8581 acgcgcgcga gccgctcagc gcgccgctca tgatcagaat gaacaccagc agggtcagcg
     8641 ccaggaacag cacagaccaa cccacctccg tcaagctgaa cccggcaatt gcgggcgcca
     8701 gatcgggcga aatcgccgtg cgcaggtgat tctcgaggtc gttgcgcgag gcgaagtata
     8761 gcagccagcc cagcaaagcc gacccgagcg ccacggccga aaagaagccc cacttgcgct
     8821 cgaagccgcg cgcttgcagc caccaggcct tgagagggac gcttgcggat ctggcggccg
     8881 ccgcggtttt cgcaaggtag agccgggcca gcgcctcgag tccgtatccc gagaggatga
     8941 cgaggctcag gctgaatgga tgcaggaact tgatcggatt gcgaatggtc gagaaatacg
     9001 gaagggcgta cagggcttga tagaagggcg catgcttgcc ccaggcgaac gcgatcgaga
     9061 gacacgcagc gcctgtccaa aaccagatcc acttccgctc ttcggaagcg aaggggccgg
     9121 cgttgccgcg tcccgcattg gccgcccccc agagcgccag caagacgacg accacacccg
     9181 cgtaaaagcc cgaacccgag tgccgtgcca gaacacccgg tcgctccccg acgcgtcccc
     9241 agtagttccc gcctcctggc gtgtcgaggc gatagccgaa gaggcccgga acaaccgccc
     9301 gaagcgtctc gactttcggc agactccact gagtggcgtc gtaccatcgc tgcgccttgg
     9361 tgcgggtgtc ctgctccatt ccgaccacac ccttgatctg cgtcgacacc agcatgctca
     9421 acgccgcgaa ggcgaccacc gcggcgcaca cggcgaccac cccgatacgc ccaatgccgt
     9481 gaacccaggc ccgccagttg gacgggtcga catgcacggc ctggaagaga acgaacgcgg
     9541 caacatagag actgagaatg gcgcccgaat cgaccccttc caggatcgcc atcccaacgg
     9601 cgaatccgcc gacagcagcc ttcagccaat cggagctggg agtcgacctg gcaagggccg
     9661 cgagggcgac gaaaatccac gccatgcaga tggtgagggt tcccagcccc cagcaggcat
     9721 aggagaagaa atccgtgttc agcgccgcgg ccagggcgcc caaaagaccg atccagggct
     9781 tgaaccgcaa ctggcggaac aacacccata cgctcaagcc aaccgtcaac acgctgagag
     9841 gggcgtaaaa cttggcgaac ccgagcggcc ccagcagcca gaaccagagg tagctgacgt
     9901 tcggaggcat attcccgccc caactcccca cccagttcaa atcccgccag atgcaacgga
     9961 agcccccggg catggacatg gcttccgaac tcgtccctcc gagcggtccg tcgctcgaga
    10021 agagtatctt ctctgggctg aagctgccgg cgaacaggaa tcctaacacc gccagaagac
    10081 agcccagggt gacgagcagc catgaaacgc ctcgaccgga cggtgctcga ggcggcgaat
    10141 cggatcgaga tcctgatctc gagttgtgga ccgtggcgcg cattgggcga taggaataat
    10201 gcagcccgtc gggaaattcc acaaaatcgt tgcagcgctg gcgtgggttg tggcttcatc
    10261 ctgctatgcg ttgtggcgcg atgcccgaat cgggcgttcg ggcctggccc ggtcctttgc
    10321 cgcaacgggc cgccgatcgt cgcgatgggc attgagggtg tcggaatatg aatcggtgcc
    10381 ggcttcatat ttggtatacg gggcacgtgc agggcgtcgg gttccgttac agcgtcaggt
    10441 ccgtcgctca gggctacgag gtctcaggct tggttcgtaa cttgttagat ggacgggtgg
    10501 aattggtggc tgaaggcgct cgagacgaac tcgatgcgtt tcgccggggc atcttggact
    10561 cgggcctgag cggcctgata cgaaatgaag aggtggtttg ggagcgggcg gagggaaacc
    10621 tgcgcggctt tgtgataagc aatgaatgac ggagttcgga gtatttgatg aagtttgcga
    10681 ttttagcaga catccacgcc aatttggagg cattggaggt tgttctggcg gacgctaaag
    10741 agcagcaggc gacgcattat gcgtgtctgg gcgacgtggt gggctactgt gccaacccca
    10801 aacagtgctt ggatatcatc cgcggcatgg ggatgccctg catcaagggc aatcacgacg
    10861 agtattgttc gatggacgag gctctcaccg gcttcaatcc ccacgcggcc caggcggtcg
    10921 agtggacacg caaccaattg acggagaatg accgcaagtg gttgcgcgat ctcaaatata
    10981 tgcggctcgt cgcaagtttc tccatcgtcc acgccactct cgacgggccc cagcgctggg
    11041 ggtatgtctt cgacaagctc gccgccgccg ccagcttcac ctaccagaac acagccctct
    11101 gtttcttcgg ccacacgcac gtccccatcg cgttcatccg ggatagcgtg gtccggggag
    11161 gcacttactc caagttccgg atcgagcccg gcaagaagta cttcgtgaac gtcggcagtg
    11221 tcggccagtc gcgcgacgga gttccccgag cgacctacgt gatttacgac atggccgagg
    11281 ggagcatcga gttgcgtcgg ttggaatacg acatggccaa gacgcaggag aaaatccgcg
    11341 cggccggtct gccccggcgg ttggcggatc gcctgagcga ggggcgttga gaccgggctg
    11401 cgtcccgctg catttgcgga cggatacaaa aaaagacggc gcgcaatgcg ccgtcttggt
    11461 gtctcttggc aagggtgcga atcagatcgg tttgggcatc gtggtccagg cgcccttctt
    11521 cttgctgctc ttcacgcacg cgtcgatgaa ggcgattccc gtccagccat cctcgatgtt
    11581 ggcgtacttg gcgccgtcgc acttgaacgc tgcgcccgcc tgatacttgc gcacgtcttc
    11641 ctcgaaacac cggtggagcc gggcgaatgc atcgtggaac gcctccgggt ggcccgatgg
    11701 aatcgtcggc tccgccatga ggctggcagg gacgtccttg ggcaggaacc cgtcgcccgg
    11761 ggtgaccgct ccgcgccagt aaacgcgatc cggctggtta gagaggtgaa tggtcaggca
    11821 ttcggggtct tcctgacgcc accggagggt gcctttggtg ccgttgatct cgattccgag
    11881 gtcgttcttg tggccgatgg caatctgggt cgcccgcacc agggctttgc tgccgttgct
    11941 cagcttgcag taaatcgtga agtgatcgtc cagcagacgg ccctcgacaa acgtctccaa
    12001 ctgtgcggag acctgcgcca cctcgagccc ggtgacatac cgaagttgca tgagggcgtg
    12061 tgtgccgata tcaccgccgc aacccgaccc gccggccgtc ttcgggttgg tccgccacac
    12121 cgcgccctgc gcgccggtgg cctcgacctt cgtcgccagc cagccttgaa ggtagtagga
    12181 atcgacccag cgcacctccc ccaggagccc ggcgcgcaca atgtagcggg agagccggct
    12241 ggtccagtgc cccaaatacg tgtgggcgac gccgaagggg atgttcctgg ctcggaccgc
    12301 tctgaccagc gcattcgcct ctttgaggtt gacagtcaaa ggcttttcgc agaagaccgg
    12361 aatgccggcc ttgatggcct tcatggccgg atcgaaatgg acgtggttgg gcgtgacaat
    12421 gaggatgtaa tcgagcttct ggtcttcagg aagcgtcttc tgcgccccga tcatttcgtc
    12481 gtacgaaccg tatcccgtga tcgggtacgg ccatttcgcc gcttcttcga gcgcaatctt
    12541 cgagtcggga aacaaggcgc cggcaacgac ccgacgcgtg ccgtcgaagt ggatggcttt
    12601 ctgatggggt tgggcgatga aggcgccacg gccgccgccc accaaaccga cattgagagg
    12661 tctgggcata agggtgttcg atctttagtg ttttgcttct tcggttcagc cgaaaccccg
    12721 gaacggtctg aagagtctcg ctgaagcttg gtg
//
LOCUS       DAOQRO010000053        12699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4064_length_12699_cov_5.638801, whole genome shotgun sequence.
ACCESSION   DAOQRO010000053 DAOQRO010000000
VERSION     DAOQRO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_12699_cov_5.638801"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /gene="murF"
                     /locus_tag="PKK20_03315"
     CDS             <1..1399
                     /gene="murF"
                     /locus_tag="PKK20_03315"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNU98948.1"
                     /translation="GMEPLSLHQVAAACRGDILAGAPDLVVRRVGTDSRQTEPGDLFV
                     ALVGDRHDGHAHVGAAVERGAIAVLGARSRRIAAPAGCALVVVDDTRRALGDLAAAYR
                     SGFDLPVVAVAGSNGKTTTKDLIAAVLAQRYASLSSEGNFNNDIGVPLTLLRVERRHQ
                     VAVVEAGTNHPGELALLLGQIQPTHGVLTSLGREHLEFFGNLAGVVDEEGWLAELLPA
                     SGTLYVNGDSPRMEDVVARTRSAVVRAGWGANADWQATGVDTDAEGMRFRVRAPRADC
                     SGTYRIRAWGRHQVVNALLGLAVGADFGLTREQMQAGLDAFRLPRLRLQLSEWRGISV
                     LEDCYNANEDSMTAALDTLRALPCPGRRLAVLGDMAELGGHAAEAHREVGRRSAGVVD
                     QLIAVGRMAPVMAQAARDAGLRDVVECEDASAAALTLAGAVRAGDLVLVKASRATRLE
                     GVIEGLKAAWAGGGI"
     gene            1406..2554
                     /gene="mraY"
                     /locus_tag="PKK20_03320"
     CDS             1406..2554
                     /gene="mraY"
                     /locus_tag="PKK20_03320"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNU98949.1"
                     /translation="MLYYLSQYLQQSAQGTGWEETLSALRLFRYITFRTAGSAVTALL
                     LSWWLGPKVIVWLKRLRFGQEYRDAADASGDLKARVLDKRGTPTMGGLLIVVVIDLTV
                     LLWAQWNALTVLTLASLVGLAGLGFYDDYAKIALQDGKGVRGRVKLWVQVLLALFIAG
                     YLWYLPDTRKLIASVIVPFYKHPITAGAPVLGMAVTVFTIVGSSNAVNLTDGLDGLAI
                     GCTLIVTAVLLIVTYVAGHAQFAEYLRVPAVSGAGELTVICGAMFGASLGFLWYNCHP
                     AQVFMGDTGSLALGGTLGIISVLAHQPFVLFIAGGVFVAEAVSVIIQVGCFKCYRRFR
                     GVGKRVFLRTPLHHHFEKLGWYESKIVARFYILCVLCAVLALASLKLR"
     gene            3017..3802
                     /locus_tag="PKK20_03325"
     CDS             3017..3802
                     /locus_tag="PKK20_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNU98950.1"
                     /translation="MNTPSPINPLSPLSQQARGKSNVRLAVISIIALHAVFFGGLLLQ
                     GCKPKTVDQSLAGRDTVTPSVPPIDVAPSVPTNVALFGPGTTQPALTGDPYAHPGVAL
                     TPSVPTNETWMTSTPPPPVESVQPTLPPSTTEYVVKARDTLGQIAKTHGISLAALLQA
                     NPGIEPLRLQIGDKVLIPAAALQSGGATPAEDADAQKTTTHVVKGGENLTRIAKTYGV
                     TVKELRAANNLKTDRILVGQKLKIPAAAPAGQMGTAETGLPPR"
     gene            3931..5043
                     /locus_tag="PKK20_03330"
     CDS             3931..5043
                     /locus_tag="PKK20_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNU98951.1"
                     /translation="MRLSTTILVASALALLSLGMLTLFSISPPNASLTYLSRQIVAAG
                     LGLGGAFLLAQIHHRRLRAWSWFLLLVAVGLLVFVMVAGVEVKAARRWCRLGPVQFQP
                     SEFAKLALLVALAHYGAVNERVMRSFWYGVVVPGMLIGLVTGLIFVEPDWGTAILLGA
                     TSMVVLVVAGARMTHLAVPVLFGMGSLCAFLAFNSVRIDRVHAWLNPDTTRQGVGFQA
                     WQAKLALGRGGLTGVGLNASLQKDPLPEEQTDFIFAIIGEEFGYVGSLVVIVLFLLYF
                     LSGVSIARQASDPFGRLLATGISFLVGLQAFINLGVVSSVLPNKGLALPFISFGGSNL
                     AIVLLCTGILVSVGRANECRVAPDPRLEELGQPQTA"
     gene            5062..6270
                     /gene="murG"
                     /locus_tag="PKK20_03335"
     CDS             5062..6270
                     /gene="murG"
                     /locus_tag="PKK20_03335"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNU98952.1"
                     /translation="MASSHIETNRCRPRIAIACGGTGGHLFPGLAVAEALRSRGADVL
                     LLTSTKEVDRNALGAAGGSEVASLEAVGLEKGGWGRFLRGFTRSLRQARALFHSRPPS
                     AVLAMGGFTSAAPILVGRRMGATTYLHEANAIPGRANRWLAHMVDQAFVNYTEAAARL
                     RHRKILVTGMPVRSALLGVDSAACRHSLGLGPDCPVLLIVGGSQGARAINQAVVRLLP
                     ALALTEPELQFVHLTGPDDIETVRRAYAAQQRRSVVEPFLIEMELALGAATVAISRAG
                     ASALAEFAALRLPAVLIPYPSAADNHQFHNAHALVVSGAARLLLQHEATPDRLIWQIR
                     ELVRSDSTRQAMSAALARWHRPDAAERIAETIFLALPDALQASAAATERSGASAPVVG
                     LRTAGSNPSL"
     gene            6267..7268
                     /gene="murB"
                     /locus_tag="PKK20_03340"
     CDS             6267..7268
                     /gene="murB"
                     /locus_tag="PKK20_03340"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNU98953.1"
                     /translation="MSTIEPRAAESGVAPGPLAELRAAHRGMGRPDAAFASELALLLS
                     GGAVIRLDEPLARRTTLRVGGVADLYVEPAQERDLAAILAFCRERRVDWLVLGRGSNL
                     VVRDGGYRGVVIGLAQPAFSAIACEGLRVSAGAGALLKAVVAAARRENLGGFEFLEGI
                     PGSLGGALRMNAGANGSWLFEILERVRIMDRRGNVLEMGSSDLAAGYRDCPFFADHIA
                     LSAVLRGEPSDPESIQNRSEGYRRRRLETQPRQPSAGCVFKNPASIAAGRLIEELGFK
                     GERVGGAQVSPIHGNFIVNVGGATARDILVLIERIRERAQSERGIALETEVKIVGED"
     gene            7299..8219
                     /locus_tag="PKK20_03345"
     CDS             7299..8219
                     /locus_tag="PKK20_03345"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNU98954.1"
                     /translation="MPLRITVMLGGPSAEREVSLRSGRAVVDALRGLGHTVEELDPLD
                     DGWQLPQKTDVVFLVLHGTYGEDGTVQRRLEVLGVPYTGSDAEASRLAFDKVLTKERC
                     VGAGVPTPRYTVLDTPDATWPPGWQPPVVLKPVCQGSSVGLQIVDSLDGLGPALAESF
                     RYDRRVLMEERIVGREVTVGILDGEVLPIIEVCPKQGPLDYRNKYTQGATECFLPARV
                     DPPTEERIRAAAFGAFRAIGGRDYARVDVMVPSAGHPVVLEVNTLPGMTQLSLLPRAA
                     AAVGYSFPGLCQKMVDLALRRAPAACRSTP"
     gene            8227..9186
                     /locus_tag="PKK20_03350"
     CDS             8227..9186
                     /locus_tag="PKK20_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNU98955.1"
                     /translation="MGLWGKPKNRRAGRGSVLDVKMRVRPVRQARRHRVVFALLVAML
                     AVVAAGLGQRGWQWARQRLMDIGLFALTSLEIATDGIWLKPEQIRQLAGIREGDNLLL
                     IDMTQVRREIELIAQVESVSVERVLPHVLRIQVKERDPILQIQAIQPEGEAGVAPAVF
                     YLDRAGVIMPPLPTLPSSPAVVRAFDALPILRGLNNADLRPGAALESRPVRAVLELLA
                     TFESSPLAREVGLAVIDISSPDVLRVTTDGGAEITLAYDRVASGLDRWRLVRQAGQRL
                     DKAVAFLDLAVTNNCPVLWFESSAVPPLKPRTEKLPRLRRKHV"
     gene            9179..10405
                     /gene="ftsA"
                     /locus_tag="PKK20_03355"
     CDS             9179..10405
                     /gene="ftsA"
                     /locus_tag="PKK20_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNU98956.1"
                     /translation="MFNNSTHLIVGLEVGTSKVCAVVGDVSPEGALSIVGLGQARSRG
                     TRKGEIVDASAVIEDVRYAIAEAEQMADVEIRSVYLGVTGSHIRSFNNRGRHTIPSVD
                     RDIGEDDVRDVIKNAKAINLPADRHTVHVVRQHFIVDSQDGVLNPVGMVGAQIEVDVH
                     VIHGITNRLQNPIRAVKSLQLEVEDIVFNGIASSLAVLTPQDKELGALVIDLGAGVTE
                     YTVYADGIIKHTGVLAVGGDHVTNDLAYGLKISMSRAEALKIEHGSALVDMGVRGRTV
                     ELPNDFGLPERSINLEHLRRIMSARLEEVFEIIAAEVERAGLLDSLRAGVFLCGGGSR
                     TPDTQKLGERVFGLPVAIGRTKTISGLVATLDQPEFATGIGLAKFGSFQHGQNSRRRP
                     WTSRLKSALTQMWTDS"
     gene            10466..11809
                     /locus_tag="PKK20_03360"
     CDS             10466..11809
                     /locus_tag="PKK20_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNU98957.1"
                     /translation="MTIETSAAEQHPMPKSAPTIQVIGVGGAGLQALDHMSRAGLDGM
                     AFAGVHTDARLLGASPLARKILLGSALTRGLGAGGDPDVARAAAEADTPALNELCAGM
                     DLVVVVAGLGGGTGSGGAPVVARVAREQGALVLGLAVLPFEFEGQRRQHQAQLALRQL
                     KTAADAVICLPNQKVSKLVDDKTTLVETFRIANDLLCQGLAGVWRLITREGLINVDFA
                     DLCRVVRGRQAESCFATAESMGEARSREVVERLLSSPLLDQGRLLADADAVLVSLAGG
                     ADLALKEVNAVMEQINRVCEHAQVIVGAAIDPALGDRIGVTVIATKRAAPVSDSERAL
                     ELSGATSDPEPPAAEFPTSGEERLDAVGGASGERPAPRYVPPPPRLTPEQAQQMLARQ
                     DRVGGRRRRKSVGMHQELLPLEVVSKGRFAKSEPTVYRGEDLDTPTYVRRGMALN"
     gene            complement(11812..>12699)
                     /locus_tag="PKK20_03365"
     CDS             complement(11812..>12699)
                     /locus_tag="PKK20_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98958.1"
                     /translation="FSGTGAGFAQFQGAGTTEASLAKSLSGQFELTTTNLNLAIASVD
                     NPMLKALVNVVVGIPDFIRNPSSGVAALVGQLTGTARQGGGFVDEMTQAPIDQIIVKG
                     AAGNGRLEVQHGYVQSAAFHANAAGTIALTAPFTNSTMRLPVQIALRRTLADRIGLAG
                     DTPTNAPFAKLPDFVTLKGTVGAPESDISRTVLLAMAAKAGAGILDRTGSPKVDQAAG
                     ILSAVGNLIGGGEGTASGTNAPAAGQSGANLLGTLNTLLGGGAQGPNAARTNTTTTNA
                     PATPARDPLGNVLDLLRKK"
BASE COUNT         1901 a         3732 c         4606 g         2460 t
ORIGIN      
        1 cgggatggaa cctctctccc ttcatcaggt cgcggcagcc tgccgcggcg acatcctcgc
       61 cggcgctccg gatttggtcg tgcggcgcgt gggcaccgat tcgaggcaga ccgagccggg
      121 cgatttgttc gtggcgctgg tgggggatcg ccatgacggg cacgcgcacg tcggggccgc
      181 cgtcgagcgg ggggcgatcg cggtgctggg ggcgcgcagc cgtcggatcg cggcgcctgc
      241 gggctgcgcc ctggtggtgg tggacgacac ccggcgggcc ttgggggatt tggcggcggc
      301 ctatcgttcc ggtttcgatt tgccggtcgt ggcggtagcg ggttcgaacg gcaagacgac
      361 caccaaggac ttgattgccg ctgtgttggc tcagcgttac gccagcctgt cgagcgaggg
      421 caatttcaac aacgacattg gcgtgccttt gacgcttctg cgtgtcgagc ggcgccacca
      481 ggtggccgtg gtcgaggcgg gcaccaatca ccccggagag ctggccctgc tgctggggca
      541 aatccagccc acgcacggag tgctcaccag tctgggtcgg gaacacctcg agttcttcgg
      601 caatctggcg ggtgtggtgg acgaggaggg ctggctggcg gagttgcttc cggcgtccgg
      661 cacgctctat gtcaatggcg actcgccgag gatggaggac gtggtggccc gcacccgctc
      721 tgcggtcgtg cgggccgggt ggggggcaaa cgcggactgg caggcgacgg gggtcgacac
      781 cgatgcggag gggatgcgtt tcagggtgcg ggcgccgcgt gccgattgca gcgggacgta
      841 tcgcatccgc gcctgggggc ggcatcaggt cgtgaacgcc ttgctggggt tggcggtagg
      901 ggcggatttc ggactgacgc gcgagcagat gcaggcgggg ctcgacgctt ttcggctccc
      961 gcgcctgcgc ctgcaacttt ccgagtggcg cgggatttcc gtgttggagg attgctacaa
     1021 cgccaatgag gactcgatga ccgcggcgct ggacacgctg cgggccttgc cgtgcccggg
     1081 gcggcgtctg gctgttctcg gggacatggc cgagttgggc gggcacgcag ccgaggcgca
     1141 cagggaggtt ggccggcggt ccgccggtgt cgtggatcag ttgattgccg ttggccggat
     1201 ggctcccgtg atggctcaag cggcgcggga tgcggggttg cgggacgtcg tcgaatgcga
     1261 ggacgcgtcg gcggctgcgt tgactctcgc gggcgcggtg cgtgcgggcg atcttgtgct
     1321 ggtgaaggct tcgcgcgcaa cgcggctcga aggtgtgatc gagggtttga aggctgcgtg
     1381 ggcgggcggc ggcatctgag gggtcatgct ctactatctg agccaatact tgcagcagag
     1441 cgcccagggg acggggtggg aggagacgct ttcggcgttg cggttgtttc ggtacatcac
     1501 ctttcgcacg gccgggtcgg cggtgacggc tcttctcttg agctggtggc ttggtcccaa
     1561 ggtcatcgtc tggctcaagc ggcttcgatt cggccaggag taccgggacg cggctgatgc
     1621 gagtggggac ttgaaagccc gggtcttgga caagaggggc accccgacca tgggggggct
     1681 tctgatcgtg gtggtgatcg atttgaccgt cctgctctgg gcgcaatgga atgcgctgac
     1741 ggtgttgacc ctggcttcgc tcgtgggatt ggcgggtctc gggttctacg acgattacgc
     1801 gaagattgcg ttgcaggacg gcaagggtgt gcgtggccgg gttaagctct gggtgcaggt
     1861 gcttttggcc ctgttcatcg cgggctacct gtggtacctg cccgacacgc ggaagctgat
     1921 cgcgtcggtg atcgtgccgt tttacaagca tccgatcacc gcgggcgcgc cggtgctcgg
     1981 gatggctgtg acggtgttca cgattgtcgg cagctcgaac gcggtcaatc tcaccgatgg
     2041 tttggacgga ctagcgatcg gctgcacgct gatcgtgacc gcggttcttc tgatcgtgac
     2101 ctatgtcgcg ggccacgcgc agtttgccga gtatttgagg gtgcccgcgg tgtcgggcgc
     2161 cggggagctg accgtgattt gcggagcgat gtttggagcg tcgctcgggt ttctctggta
     2221 caactgccat ccggcgcagg tgtttatggg ggacaccggc tcgctggcgc tgggggggac
     2281 actcgggatc atctcggtgt tggcgcatca gccgtttgtt ctgttcattg cgggcggcgt
     2341 ctttgtcgcc gaggcggtct cggtgatcat ccaggtcggt tgtttcaagt gctaccggcg
     2401 ttttcggggc gttgggaagc gcgtgttcct gcggacgccg ctgcatcatc acttcgaaaa
     2461 gctgggctgg tacgagtcga agatcgtcgc ccgtttctac atcctgtgtg tgctctgcgc
     2521 tgtgctggcc ctggcgagcc tgaagctgcg ttgaacggga accgtgcgag gacatgaagc
     2581 cacagacaac aacggggaaa tcgaaggcaa tcgagcgcgt acgttccggc ggatgcagag
     2641 cggcagccgc cccgggccgg caggcctggg cgtgggggag gaatgcgttt gccgcggctc
     2701 aaaatgccgt gttcggcgtt gcaggtttgc cctcgccggt ttattcgtat tccgagatgt
     2761 ttccggacgg ccaacatcag ggtgaggtgt ctcagcccgg ctgcgaacac gccgcgtggt
     2821 cggccacgcc gcatgctcga cgggaggcgg agtccgccct cgctgtccag gggatgcgca
     2881 gcggtcgacc actgcacgag agccatccgg ggtccgatct tttgcctcga cgttttttga
     2941 gggaaaacgc cggggcgaaa ctacaaccga gtcaactcct caactccaga tccgcgcagc
     3001 cgcgcgctcg catcctatga acacgccaag tccaatcaat cccttgagtc cgctgagcca
     3061 gcaggccagg ggcaagtcca atgtccgcct cgcagtgatc tcgatcatcg cattgcacgc
     3121 ggtgtttttt ggcggccttc tgcttcaagg ctgcaaaccg aagacggtcg accagagcct
     3181 tgcgggccgt gacacggtga ccccgtcggt tccgcctatc gatgtcgccc cgtccgtgcc
     3241 caccaacgtg gcgctgtttg gtccggggac aacccagccg gccttgacgg gcgatcccta
     3301 tgctcacccg ggggtggcgt tgaccccgag cgtgccgacc aacgagacgt ggatgacatc
     3361 gacgccaccg ccgcctgtgg aatcggtgca gccgacgttg cccccgagca ccaccgagta
     3421 cgtggtcaag gcgcgcgaca ccctgggcca aatagccaag acccatggca tctcgctcgc
     3481 tgcgctcttg caggccaacc caggcatcga gcccctccga ttgcagatcg gcgacaaggt
     3541 cctgatcccg gccgccgcct tacagagcgg cggggcgact ccggctgagg atgcggacgc
     3601 tcagaagacg accacgcacg ttgtgaaagg cggagagaac ttgacccgga tcgccaagac
     3661 ctacggggtc accgtcaagg aactccgcgc cgccaacaac ttgaagactg atcggattct
     3721 cgttggtcag aaactgaaga ttccggccgc cgcgcccgcc ggacagatgg gcacggctga
     3781 gaccggactt ccgccgcgct aggggcgcga gcgccccggc aggcagcgcg gcgccggccg
     3841 tcctcaagcc ggttccgtgt tctgccgccg gggcgcccgg catcaccgtg tgcggttgcg
     3901 gcgtgggcaa agaatgaaat cgagtctgtg atgaggcttt ccacgacgat cctggtggcg
     3961 tccgcgctgg ccctgctgtc gttggggatg ttgacgctct tcagcatcag cccgcccaac
     4021 gccagcctga cgtacctgtc gcgtcaaatc gtggcggcgg ggctcgggct ggggggtgcg
     4081 tttctgttgg cccagattca tcaccgccgg ctgcgtgcct ggagttggtt cctgctgctg
     4141 gtggcggtcg gattgcttgt ctttgtcatg gttgccggcg tcgaggttaa ggccgcgcgc
     4201 cgttggtgcc gcctgggtcc ggtccagttt cagccgtcgg agtttgccaa gctggcgctg
     4261 ctggtggcct tggcgcacta cggggccgtg aacgaacggg tgatgcgctc tttctggtac
     4321 ggcgtcgttg tgccgggcat gctcattggc ctggtcacgg gtttgatctt cgtagagccg
     4381 gattggggga cggcgattct gctgggggcg acgtccatgg tggtcctggt ggttgccgga
     4441 gcgcgcatga cgcacctggc ggtacccgtg cttttcggga tggggtctct ttgcgctttc
     4501 ctggccttta attccgtgcg gattgaccgg gtgcacgcct ggctcaatcc cgacaccacg
     4561 cgccaggggg tgggttttca ggcgtggcag gctaaactcg ccctggggcg gggcggactg
     4621 accggtgtgg gtctgaacgc gagcctgcag aaggatccgc ttcccgagga gcagaccgat
     4681 ttcattttcg ccattatcgg agaagagttc ggatacgtcg ggtccctggt tgtgattgtc
     4741 ctcttcctgc tctatttcct gagcggtgtc agcatcgccc gtcaggccag cgacccgttt
     4801 ggccggctgt tggcgacggg gatcagtttc ctggtcggtc tgcaggcatt catcaacctc
     4861 ggtgtggtgt cctcggtcct gcccaacaaa ggcctggctc tcccattcat cagcttcggc
     4921 ggttcgaatc tggccatcgt gcttctttgc accggcattc tggtgagcgt gggccgggcc
     4981 aacgagtgcc gggtggcgcc cgatccgcgt ttggaagagc tcggacaacc gcaaacggct
     5041 tagttcaaag actctgcacc catggcctca tcacacatcg agacgaaccg ctgccggccg
     5101 cggatcgcca tcgcatgtgg cggcacgggc gggcacctct ttccgggatt ggcggtcgcc
     5161 gaggcgctgc ggtcgcgggg ggctgacgtt ctcttgctga cttcgacgaa ggaggtggac
     5221 cgaaacgccc tgggtgcggc cggtggaagc gaggtggcct cacttgaagc ggtcggcctc
     5281 gagaagggag gatggggacg atttctgcgc ggattcacgc gatcgctcag acaagcgcgc
     5341 gcgctgtttc acagccgtcc gccctcggct gtgctggcga tgggcggatt caccagtgcg
     5401 gcgccgatcc tggttgggcg gcgcatgggg gcgacgacct atcttcacga ggccaacgcc
     5461 attccgggcc gtgccaaccg ctggctggct catatggttg atcaggcctt tgtaaactac
     5521 accgaggccg ccgcgcgcct gcggcaccgg aagattctgg tcaccggcat gccggtgcgg
     5581 tcggctttgc tcggcgtcga ttctgccgcc tgtcggcaca gcctcgggtt ggggccggat
     5641 tgtcctgttc tcttgattgt ggggggaagc cagggggcac gcgcgatcaa ccaggcggtg
     5701 gttcgtctgc tgcccgcttt ggcgctgacc gagcccgagc tgcaatttgt ccacctgaca
     5761 ggccccgacg acatcgaaac cgtgcggcgg gcctacgccg cacagcagcg gcgttctgtg
     5821 gtcgagccat tcctgatcga aatggagctg gcgttggggg cggcgaccgt ggcgatttcg
     5881 cgcgcggggg cgtcggccct agccgagttc gccgccctgc ggctgccggc cgtgctgatt
     5941 ccctatccgt ccgccgccga caaccatcag tttcacaacg cccatgccct ggtagtgtcc
     6001 ggcgcagcgc gtctcctact ccagcacgag gcaacgcccg atcggctgat ctggcagatt
     6061 cgcgaacttg tgcgcagcga ctcgactcgg caagcgatga gcgcggcttt ggcgcgatgg
     6121 catcggcccg acgcagcaga gcggattgcg gagacgatct ttctcgctct gcctgacgct
     6181 ctgcaggcgt ccgcggcggc caccgagcgg tcgggtgcgt ctgcgcccgt tgtgggattg
     6241 agaacggcgg gctcgaatcc ctcgctatga gcacgatcga gccacgcgcc gcagagtcag
     6301 gggtcgcccc aggcccgttg gccgagttga gggctgcgca ccgagggatg gggcggcctg
     6361 acgcggcttt cgcgtcagag cttgcgctcc tgctgtctgg cggggcggtc attcggctcg
     6421 acgaacccct cgcgcggcgc acgacgttgc gggttggtgg ggtggcggac ctttatgtcg
     6481 agccggccca ggagcgggat ttggctgcga ttttggcgtt ttgccgggag cggcgagtcg
     6541 attggcttgt gttggggcgc ggatcgaacc tggtggtccg ggatggcggg tatcgtggcg
     6601 tggtgatcgg tcttgcgcag ccggccttca gcgcgatcgc gtgcgagggt ttgcgggtga
     6661 gtgccggggc gggggctctg ttgaaggcgg tggtggcggc ggcgcggcga gagaatctcg
     6721 gcggattcga gtttctcgaa ggcatccccg ggagtctggg tggggcgttg cggatgaatg
     6781 ccggggcgaa cgggagttgg cttttcgaga ttctcgagcg tgtgcgaatc atggatcggc
     6841 gtgggaatgt gctcgagatg ggatcgtccg atctcgcggc cgggtatcgc gactgtccgt
     6901 tctttgcgga ccacattgcg ctgtcggctg tcctgcgcgg agagccctcg gaccccgagt
     6961 cgattcagaa ccggagcgag ggttacaggc ggcggcggtt ggagacgcag ccccggcagc
     7021 ccagcgccgg gtgtgtgttc aagaatccgg cgtcgattgc cgccggaaga ctgatcgagg
     7081 agctgggatt caagggcgag cgtgtgggag gggcgcaggt ctcgcccatc cacggcaatt
     7141 tcattgtgaa tgtcgggggc gcgaccgccc gggacattct cgtattgatc gagcggattc
     7201 gcgagcgcgc gcagagcgag cgggggattg cgcttgaaac cgaagtcaag atcgtcggag
     7261 aggattagcc gagagccggc ctgtggccgg cagactgtat gccattgagg atcactgtga
     7321 tgctgggagg gccatcggcc gagcgcgaag tttcgctgcg gtctggccgg gccgtcgtcg
     7381 acgccttgcg cgggctgggc cacaccgtcg aggaattgga tccgctcgac gacgggtggc
     7441 agttgccgca gaagacggat gtggtgtttc ttgtgctgca cgggacgtac ggcgaagacg
     7501 gcacagtgca aaggcggttg gaggtgttgg gagtcccgta cacggggagc gatgccgaag
     7561 ccagccggct ggcgtttgac aaggtcctca ccaaggagcg ttgtgtgggc gccggtgtgc
     7621 cgactccgcg atacacggtc ctggacacgc cggatgcgac ctggccgcct ggctggcagc
     7681 cgcccgttgt tttgaagccg gtgtgtcagg ggtcgagcgt ggggctgcag atcgtcgact
     7741 ccctggacgg tctggggccg gcgctggcgg agtcgttccg gtacgatcgc agagtgctga
     7801 tggaggagcg cattgtgggg cgcgaggtga ccgtaggcat tctggacggc gaggttttgc
     7861 cgatcatcga ggtctgcccg aagcagggtc cgctcgatta ccgcaacaag tacacgcagg
     7921 gggcgaccga gtgctttctg ccggcgcgag tcgatccgcc gacggaggaa cggattcggg
     7981 cggcggcgtt tggggcgttc cgggcgattg gcggacggga ctacgcgcgg gtcgacgtga
     8041 tggtgccgtc ggcggggcat ccggtcgttc tggaagtgaa cacgttgccg gggatgacgc
     8101 aactgagttt gttgcctcgg gcggcggcgg ctgtgggcta cagttttcct ggcctgtgcc
     8161 agaagatggt cgacctggcg ctcaggcgcg cgcctgcggc gtgccggtcc actccttgaa
     8221 aggcctatgg gactttgggg caaaccaaag aaccgccgcg ccgggcgcgg gtcggtgctt
     8281 gatgtgaaga tgcgcgttcg gcctgtgcga caggcgcgtc ggcaccgggt tgtgttcgcg
     8341 ctgttggtgg cgatgttggc ggttgtggca gccgggctgg ggcagcgggg ttggcagtgg
     8401 gcgcggcagc gtctgatgga catcggtctg tttgccctga cgagcctgga gatcgccacg
     8461 gatggaatct ggctcaagcc ggagcagatt cgccaactgg ccggaattcg ggagggcgac
     8521 aatctgctgt tgattgacat gacccaggtg cgtcgtgaga tcgagttgat tgcccaggtg
     8581 gaatcggtgt cggtcgagcg cgttctgcct catgttcttc ggattcaggt gaaggaacgc
     8641 gacccgatcc tgcagattca ggccattcag cccgaaggcg aggccggcgt ggcgcctgcg
     8701 gtcttctatc tggaccgcgc gggcgtgatc atgccgccct tgccgacgct cccgtcgagt
     8761 cccgcggtgg ttcgggcatt cgacgccctg cccattctcc gggggttgaa caacgccgat
     8821 ttgcgccccg gcgcggccct tgagagtcgt cctgtgcgcg ccgttctcga actgcttgcc
     8881 acgttcgagt cgtcgccgct tgcccgggag gttggtttgg cggtgattga catctcttcg
     8941 cctgacgttt tgcgggtgac gaccgacggg ggggcggaga tcacgctggc gtacgaccgg
     9001 gtggcgtccg gcctcgatcg ctggcgtctg gtgcgccagg cgggccagcg attggacaaa
     9061 gctgtggctt ttctggattt ggccgtcacc aacaactgtc cggtgttgtg gttcgagtcg
     9121 agcgcagtgc cgccgctcaa gccgcgcacc gaaaaactgc ctcgtttgcg tcgcaagcat
     9181 gtttaacaat tccacgcatc tcatcgtcgg gctcgaggtc ggaaccagca aggtgtgcgc
     9241 cgtcgtcggc gacgtcagcc ctgaaggcgc cttgagcatt gtcggtctgg ggcaggcccg
     9301 gtcccggggg acgcgcaaag gggagatcgt cgacgccagt gcggtgatcg aggatgtgcg
     9361 ctatgcgatc gcggaggcgg agcagatggc cgatgtggag attcggagtg tctacctcgg
     9421 cgtgacgggc agccacatcc gaagtttcaa caaccggggg cgccacacga tcccgtcggt
     9481 cgatcgcgat attggcgagg acgacgtgcg ggacgtgatc aagaacgcca aggcaatcaa
     9541 cctgccggcg gatcggcaca cggtccacgt ggtgcgccag cacttcatcg tcgacagcca
     9601 ggatggcgtg ctcaacccgg tcggcatggt gggcgctcag atcgaggttg atgtccacgt
     9661 gatccacggc atcaccaacc gcctccaaaa cccgatccgg gccgtcaaga gccttcaatt
     9721 ggaggtcgag gatattgtgt tcaacggcat cgcctcgtcg ctggccgttt tgacgcctca
     9781 ggataaggag ttgggcgccc tggtgatcga tctcggcgcg ggtgtcaccg agtacacggt
     9841 ctatgcggac ggcatcatca agcacaccgg cgttctggcg gtcgggggcg accatgtgac
     9901 taacgatctg gcgtatgggc tcaagatatc gatgagccgc gctgaggcgt tgaagatcga
     9961 gcatggctcg gcgttggtcg atatgggcgt gcgggggcgg actgttgaat tgcccaacga
    10021 tttcggcctg cccgaacggt cgatcaacct cgagcacttg cgacggatca tgtcggcccg
    10081 gctcgaggag gttttcgaga tcatcgcggc cgaggtcgaa cgcgcggggc tgctcgacag
    10141 tctgcgggcg ggtgtgttcc tgtgcggggg cggctcgcgc acgccggata ctcagaagct
    10201 gggcgagcgc gtctttgggt tgcccgtggc cattgggcgc accaagacga tcagcggcct
    10261 tgtggccacg cttgatcaac ccgagttcgc gacgggcatc ggcctggcga agttcggatc
    10321 gtttcagcac gggcagaact ctcgccggcg tccctggacg agccggctca agagcgcttt
    10381 gactcagatg tggaccgact cctgatcccg aggcggcgcc ccggtttttg tccaagccgc
    10441 ctcttcaacc gtttcgcaac accccatgac aatcgaaact tcagccgccg aacaacaccc
    10501 gatgccgaag tccgccccga ccatccaagt gatcggcgtg ggcggcgccg gccttcaggc
    10561 gctcgatcac atgagccgcg ccggactgga cggcatggct tttgccggag tccacaccga
    10621 cgcgcggctc ctgggggcga gtccgctcgc ccggaagatt ctcctcgggt cggcgttgac
    10681 gcgcggcctg ggcgcggggg gcgaccccga cgtggcgcgt gcggcggccg aggcggacac
    10741 cccggcgctc aacgagcttt gtgcggggat ggatttggtt gtggtggtgg cggggttggg
    10801 cggcggcacc gggtcgggcg gggcgccggt ggtggcgcgc gtggctcggg aacagggggc
    10861 gttggtgctg ggtttggcgg tcctgccatt cgagttcgag gggcagcgtc ggcagcacca
    10921 ggctcaactg gccctgcgcc agctcaagac ggcggcggac gctgtcatct gcctgccaaa
    10981 ccagaaggtg tccaaactgg tcgacgacaa gacaacactg gtcgagacct tccgcattgc
    11041 caatgacctg ctttgccagg ggctggccgg cgtgtggcgc ctgatcaccc gcgaaggcct
    11101 gatcaacgtg gattttgcgg atctctgccg tgtggtgcgg gggcggcagg cggagagctg
    11161 cttcgccacc gccgagtcca tgggcgaagc gcgctcgcgc gaggttgtcg agcgcctcct
    11221 ctcgagtcct ctgctcgacc aggggcgtct tctggccgat gccgatgccg tcctggtcag
    11281 cctggcgggc ggcgctgatc ttgcattgaa agaggtcaac gcggtcatgg aacagatcaa
    11341 tcgcgtgtgc gagcacgccc aggtgatcgt gggcgccgcc atcgaccccg ccttgggcga
    11401 tcgcatcggg gtcaccgtga ttgcgaccaa gcgggccgcc cccgtttccg attcggaacg
    11461 cgcgctcgag ttatccggag caacgtcaga ccccgaaccg cccgcggccg agtttcccac
    11521 atcgggggaa gagcgcctcg atgccgtggg aggcgcgtcg ggcgagcgtc cagctccccg
    11581 gtacgtcccg ccgccgccgc gtctgacgcc ggaacaggcg cagcagatgc tcgcccggca
    11641 ggaccgcgtc ggggggcgcc gccggcgcaa atcggttggg atgcaccagg agttgctgcc
    11701 actcgaagtg gtgtccaagg gccggttcgc caagagcgag ccgaccgtct atcgtgggga
    11761 agacctggac accccgacgt acgtccgccg gggcatggcg ctcaactgat cctacttctt
    11821 gcgcagcaga tccaacacgt tgcccagcgg gtcgcgggcg ggcgttgccg gcgcattggt
    11881 ggtggttgtg ttcgtcctgg cggcgtttgg gccttgagcg ccgccgccca gcagtgtgtt
    11941 gagcgttccg aggagattgg cgccgctttg gcccgcggca ggcgcgtttg ttcccgatgc
    12001 ggtgccttcg ccgccgccga tcaggttgcc gaccgcgctc aggattccgg cggcctggtc
    12061 gaccttgggc gaaccggtcc ggtcgagaat ccccgcgccg gctttggcgg ccatcgccag
    12121 gaggacggtc cggctgatgt ccgattccgg cgcgcccacg gtgcccttca aggtgacgaa
    12181 atccggcagt ttggcgaagg gcgcgttggt gggcgtgtca ccggccaatc cgattctgtc
    12241 cgccagtgtt cggcggagcg cgatctgaac gggcaggcgc atcgtcgagt ttgtgaacgg
    12301 ggcagtcagc gcgatggtgc cggcggcgtt tgcgtggaaa gcggcactct ggacataccc
    12361 atgttgcacc tccaaacgcc cgttgccggc ggcgcccttg acgatgatct ggtcgattgg
    12421 agcctgggtc atctcgtcca caaagccgcc accctggcgg gccgttccgg tcagttggcc
    12481 cacgagcgct gcgacgccgc tcgagggatt gcggatgaag tcagggatgc cgaccaccac
    12541 gttgacgagc gccttgagca ttggattgtc cacactcgcg atggccaggt tcagattggt
    12601 tgtggtgagt tcgaactggc cggaaaggct cttcgccaga ctcgcttccg tggttccagc
    12661 gccctggaac tgtgcgaagc ccgcgccggt gccgctgaa
//
LOCUS       DAOQRO010000054        12667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4083_length_12667_cov_5.588170, whole genome shotgun sequence.
ACCESSION   DAOQRO010000054 DAOQRO010000000
VERSION     DAOQRO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12667
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_12667_cov_5.588170"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="PKK20_03370"
     CDS             complement(<1..1540)
                     /locus_tag="PKK20_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU98959.1"
                     /translation="MEQNIHRKGLVNWLLLLAGAVGALVLARYCHSATGLVAGVFMAL
                     GFLVAAVSYFQMRLEYRERLEQMEFDELNRTSSSRTLFTEVQADTFPARRAREQFERF
                     IVPAFALVLFLLQAGAFYLLWKHIRDDQPATGQQATVAMAINALLALVLFQFGKYSAV
                     LSQLEKQRLLRPQASYLLLGALLAIVTAAVEVAGWADYPRVDVIVARVLTIVLGLVAV
                     ENLLSLVFETYRPRVKGQAEHPLYESRLIGLLSQPGGLITTAAQALDYQFGFKVSETW
                     FYRYLERALAWLILGQVAVLVLSTSFAFVELGEHGLLERFGRPVAGREVLDPGLHVKW
                     PWPIDKVYRFRTDQIQTFLIGLESDADYEKDRVLVWTKSHAKEEAPWLVASKAQQTTN
                     AAPGEVAVPVNLLAVNIPVQYQVHDVRAFAYNHVDAGNLLEDLANEVVTRYLVSVDID
                     EVMTFGRKAAAEDLRRRIQERADALQLGVKVLFLGLHGIHPPVKVAPDFERVIGALQD
                     REATN"
     gene            complement(1553..2476)
                     /locus_tag="PKK20_03375"
     CDS             complement(1553..2476)
                     /locus_tag="PKK20_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98960.1"
                     /translation="MELVRQPVFLLLMTASGAFGVFLAVVPYFGFGDDPKMVKDSVMA
                     VMLVAGLLGAVLSASSSLARELRTGTALAVLAKPVSRAQFLTAKYTGVAAALSVLTYF
                     NLISALLASRMAYDAYGSTDTVALRIYAVAIVLAYAVAAFANYFLNRTFVSDAVWGLL
                     AFGTVAFCVINSFDKEGALQKFAQGVDWRLLPATVLIVFAVWILAGLALACSTRFEMI
                     PTLAICTAVFVLGLMSDYLFGIRASEGQWWAGICHTVLPNWQIFWMADALDQAKTIPW
                     SYVGRAFGYVVGYLGAALALALLFFEDRELS"
     gene            2735..4180
                     /locus_tag="PKK20_03380"
     CDS             2735..4180
                     /locus_tag="PKK20_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNU98961.1"
                     /translation="MQSLAATGFARRTRRLWPALAAIATCLCLAVSARAGDVPLIGTI
                     EWRASDNLVDAQIEGWPLQKVLEQIAARTSWHVYAEPGAQHVISAKFKNLKPGDALRR
                     LLGGLNFALIPSNSGPARLYVFLSSQAGATVRIEAPDQARTEGQAKRSVPNELLVTLK
                     PGAKESIDDLAKRLGARVVGRLDALGAYRLEFEDSAATDAARAALAENSDVSAIEENL
                     GFARPEVPQVQASGTAPPLVLRPRAVTDGSQVVIGLIDTPVQQQGTILKDFLLPGVSV
                     AGDSAIAADTLSHGSAMATTILYGLARSSGASIDTPVRILPVDVYGASGSTTTFQLAE
                     GILEAINRGASIINLSLGGETQSPLLESVIQAGHAQGAVFLAATGNQPVTTPTYPAAY
                     SQVIAVTALGQHGEIASYANRGDFVDAAAPGISVVPFQGQSYVVVGTSTATANASAYA
                     AHVMATTGTRGSQLDALVRQSLGVTPTAGKP"
     gene            complement(4224..4580)
                     /gene="folB"
                     /locus_tag="PKK20_03385"
     CDS             complement(4224..4580)
                     /gene="folB"
                     /locus_tag="PKK20_03385"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNU98962.1"
                     /translation="MDTIIIRDLELFLRVGVSEDERAKPQRLLVSIEIAHDFADAAEK
                     DDLRSSIDYAAVCQRLAEWGAGRSWCLIETLAVEMADLLLREFGATTATVEVRKFVLP
                     QTRFVGVRVTRMFHTP"
     gene            complement(4580..5662)
                     /locus_tag="PKK20_03390"
     CDS             complement(4580..5662)
                     /locus_tag="PKK20_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNU98963.1"
                     /translation="MIRNVIFDWSGTLVDDLPAVLAASNHVFRQAGVPEMTLEQFRSE
                     FCLPFKTFYDRYVPHVPWPQLEEWFHSAFRQSQHLVRELPHAREFLEFCRRHGLRTFL
                     LSSVHRDHFAAQAAETGFGRYLDHPYVEVWDKRAKIHQLLGDHGLAPRQTVFIGDMEH
                     DVETARCGGVYACAVLTGYNNLAQLRAGQPDLIVEHLGELRDLLELQGFRIEGAAPAA
                     RKRTPAGPVATVGALVFNQAGEVLMVRTRKWSNLWGIPGGKIKLGERCADALRRELKE
                     ETDLEVRDIAFVMVQDCIHSREFYRDAHFLLLNYTCRCAGPAVVRLNAEAQEYRWVTP
                     PAAMDLPLNQPTRTLLTTYLAQAGPQ"
     gene            complement(5794..10332)
                     /locus_tag="PKK20_03395"
     CDS             complement(5794..10332)
                     /locus_tag="PKK20_03395"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HNU98964.1"
                     /translation="MNCLSFNFRTVRSVVGRAAALGGLAGLCTAMAASEVLWLDNFNT
                     TEPTWDVNYQINTRVSGSVGALPYVEVPDTAANGTYDDRTAVNTAEYPGTLALTAVNG
                     GWTSISPNHSFIEGGHFSVEFDLDPGVNDLGSSSGDWAAIVLGATARNMFVNASDGFG
                     ILFRHNGAWQVFNRTAAVYGSPAGTTLPTGEAFHVKIDVSSDGFRGTNTPVAISVSVN
                     GQPLTITSDPSSPTVLVNTNGFLANYITLLNYADAAQTWIDTFDNFQISGEPCVTASP
                     SRVFAKVGGNDQAVTVRIPKSMNASAPATVTLTSADPAVAVPAGAVGGSLEVTFAAGA
                     APTKTVNLVGVGRGTTEIALSNGAGVCVGDPVRVTVYPASPAVWCDEFQSEPFDPFYR
                     LNLAPFETGSAFGQVMVNPATGIEFSLYADGSYWGGQSLATEKTFSASPEAPLTLEID
                     RVLHESYGTLSGTSTRSGVWITDQTRSRYVFFSYNTGEGGWEYNRKIGLAGDSATGNG
                     IDIPAFDYLPFEDGGLHRMKMVADGATVKLFLDGLMGVDVPFPVSDGIVFEFGAYTRA
                     PDDMVTAIFDNLCIYSDNAAAEPCVAGSPSLISGTVGGTATVTIRVSAHLTATKPVTV
                     TVTSADPRVATPASGAGGALTLMFPVGGPSEQTFDLNLVGGGITTLQIANSAGACNFP
                     TAISVKVSGAFVRNPSFESNYNATWPSYSAIDNWDGGSGVNQGATGPFADNGTIPDGN
                     RVGFKQGSGSVSQTISGLEPGKEYWAQIWYNRRNCCGDVLDDMTLQFEGVEIGSVKGV
                     VPVGGQNPYGFRNFAFTPSSASGLFEIVHTVHSGDASLVFDAVTIVQRDAGNVVVRNP
                     SFEASGELAGEGVLARIAGWTTEGTTGINFSGLGVYANNGANPDQDLVAFIRGPGALS
                     QTILGLLPGQNYKVTFAYNAPLGQAPRLRVSADGASLLEVDVTPVGGSAAYWVGSTSF
                     TAASDTAVLRFEQVVADPTQTVLLDDVKVTGVSINVPCIEVRPAALQLAVGETDSAGL
                     TVSVPGELLQLSYPARVTVSSLNPAVARVEGAVGGVLTLEFPVGGDPEALSATVTVTG
                     VSKGVTALQFANPHGVCFNRKEVSVLVLGSFVRNPSFEADEVPGYPGYMAIQSWPSEG
                     SGNTGINPVQADGPFSDNGATPDRDLVALLQTSKIIRQNIMNLTPGKTYWLQFYYNVR
                     NCCGGTIDLTASFDDLPLGTIAAITPVGGSNLPDGPSYYFQQYEFTPAAASGLLAFET
                     TASGDATVLLDAITIVQRDPDNIPVVNPSFEASGVVPLPGTITKNIAGWTAGGAGNRG
                     INTSGLGFADNGANPDQDNVAFLQGAAWLSQTITGLTPGQNYTVSVAVNASTGTTPIL
                     KISADGEEAATEAITPVGGSQPYAVKTAVFQAAGTEAVIRLEQVAGGDQVLLLDDVKV
                     TPGGTLPVKASLKVGMTSTGTVRVSWPATAIGFSLQVTGALPGGWADSTAPVVAEGAE
                     LAAYIEPSANAQFFRLRK"
     gene            10592..11989
                     /locus_tag="PKK20_03400"
     CDS             10592..11989
                     /locus_tag="PKK20_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNU98965.1"
                     /translation="MILPFGALLAAIALAPLVCANWWARHYPKVALGLGAVTLGYYLF
                     WIHAHERVEHTAIEYVSFIVLIGALFVVSGGIHITVQGEATPRANCVFLLIGAIISNA
                     LGTTGASMLLIRPWLRMNAYRITAHHVVFFILIVSNVGGCLTPIGDPPLFLGYLRGIP
                     FWWVAQHCWPMWAVGVGALLLLFYVVDRCNYVRAPREVRKKLAEPPDRWRFEGLVNLV
                     FLGAILGAVFINRPPFLREAIMLAAAVASYYSTRPAIHQSNHFNFHPIREVAILFIGI
                     FATMMPALDWLEMNAARFGEPRAGQFYWACGMLSSGLDNAPTYLSFLSACLGSLVDAD
                     VVEQVRAILAAGGSLAAVPDGPNAEAVRQTLDTLRRFYPVAVAAGSVSPNQIEMAVLL
                     GDPHLNRFIIAISIGAVFFGANTYIGNGPNFMVKTIADQRRVRTPTFLGYIFRFTLPF
                     MMPMVLLVWLLFFRG"
     gene            complement(12075..>12667)
                     /locus_tag="PKK20_03405"
     CDS             complement(12075..>12667)
                     /locus_tag="PKK20_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98966.1"
                     /translation="SMRAVRGEGVVCVRLDRDGRETVLCRQPEGKRGASRMVVAAAPV
                     VDEDRGVVFFCVNGDRETTLHAWSLRDERVLWTRQMPRFTVATPALRPDGAVVVAGMD
                     GAVRAFAVGGAPLFSYATGADYLLAGPVCDAAGTAFVGDPLGRLHAVDQNGAGRAVFE
                     APRSIQARPAFDWNGHLHVACTDRTVRVFRNRLVRV"
BASE COUNT         2283 a         4283 c         3913 g         2188 t
ORIGIN      
        1 ggttggtggc ctcgcggtcc tggagcgcac cgatgactct ctcgaaatcc ggcgcgacct
       61 tgaccggcgg atggatgccg tgcagtccca ggaagaggac cttgactccc aactgcaggg
      121 catccgcgcg ctcctggatg cggcggcgca aatcttcggc agccgccttg cgtccgaacg
      181 tcatgacctc gtcgatgtcg acgctgacca ggtaacgcgt caccacttcg ttggccaggt
      241 cttcgaggag gttcccggcg tcgacatggt tgtaggcgaa ggcccgcacg tcatggacct
      301 ggtattgtac cgggatgttc accgccaaca ggttcactgg cacggccacc tcgccgggcg
      361 ccgcattggt tgtctgttgc gccttgctgg ccaccagcca gggcgcctcc tctttggcgt
      421 gcgacttggt ccagaccagg acgcggtcct tctcgtagtc cgcatcggac tcgaggccga
      481 tcaggaacgt ctgaatctgg tcggtccgga accgatagac cttgtcgatg ggccagggcc
      541 atttcacatg gaggcccgga tcgagcacct ccctccccgc gaccggacgt ccgaagcgtt
      601 cgagcaggcc gtgctcgccc agttcgacaa aagcgaagga cgtcgacagc accagcacgg
      661 cgacctggcc gaggattaac catgccagcg cgcgttcgag gtaccgataa aaccaggtct
      721 ccgagacctt gaatccgaac tgataatcca aggcctgggc cgccgtggtg atcaatccgc
      781 ccggctggct caacaggccg atcagtcggc tctcgtagag cggatgctcc gcctggccct
      841 tgacgcgcgg acgataagtt tcgaacacca ggctcagcag gttctcgacc gccaccaacc
      901 cgagcacgat cgtcagcacg cgcgcaacga tgacatccac tctcgggtag tccgcccaac
      961 ccgccacctc gaccgcggcc gtcacgatcg ccaacagcgc gccgagcagg aggtagctgg
     1021 cctggggacg caggaggcgc tgcttttcga gttggctcag gacggcggag tatttgccga
     1081 actgaaagag aaccagggcg agcagggcgt tgatcgccat cgccacggtt gcctgctgcc
     1141 ctgtcgcggg ctggtcgtcc cggatatgct tccacaggag gtaaaacgcg ccggcctgca
     1201 gcaggaacaa cacgagagcg aacgccggca cgatgaaccg ctcgaattgc tcccgcgcgc
     1261 gccgggccgg gaaggtgtcg gcctgcacct cggtgaaaag tgtccggctc gacgatgtcc
     1321 ggttcagctc gtcgaattcc atctgctcga gccgttcccg atactcgagg cgcatctgga
     1381 agtaactcac ggccgcgacg aggaacccga gggccatgaa caccccggcg accagtcctg
     1441 tggcggaatg gcagtagcgc gccaaaacaa gggcgcccac ggcccccgcc agcagcagca
     1501 gccaattcac cagccctttg cgatggatgt tctgttccat gactcacctg cttcagctca
     1561 actcgcgatc ttcgaaaaac agcagggcca gcgctaacgc cgcacccaga tacccgacga
     1621 catatccgaa ggcccgtccc acataactcc atggaatggt ctttgcctgg tccagcgcgt
     1681 ctgccatcca gaaaatctgc cagttcggaa gcaccgtgtg gcagatgccg gcccaccatt
     1741 ggccttcgga cgcccggatg ccgaaaagat agtcggacat cagacccagc acaaataccg
     1801 cggtgcaaat ggccagggtg gggatcatct cgaaacgggt cgaacaggcc agggcgagcc
     1861 cggccagaat ccaaacggcg aagacgatca ggaccgtcgc cggcagcagc cgccaatcca
     1921 cgccctgagc gaacttctga agagcccctt ccttgtcgaa ggaattgatc acgcaaaacg
     1981 ccacagtgcc aaacgccagc agcccccaca ctgcatccga cacgaaagtc cggttcaaga
     2041 agtagttcgc aaacgcggcc acggcgtagg ccagcacgat cgccaccgcg tagatgcgga
     2101 gggcgacggt gtcggtcgat ccgtaagcgt cgtacgccat ccggctggcc agcagcgccg
     2161 agatcaggtt gaagtaggtg agcacggaca gggccgcggc gacgccggtg tactttgcgg
     2221 tgaggaactg ggcgcggctg accggcttgg ccaacacggc cagcgccgtg cccgtgcgca
     2281 attcgcgagc gagcgacgat gaggcgctca ggaccgcgcc gagcagaccg gcaaccagca
     2341 tgactgccat gacgctgtcc tttaccattt tcggatcgtc gccgaatcca aaatacggca
     2401 cgaccgccag gaacacccca aaggcgccgg aagccgtcat cagcagcaga aagaccggct
     2461 gccgaaccag ctccataaag gcattgctcg caatggttag gaattgtcgc attcccagtg
     2521 ttcgccgtcc gcaaggttac gaatccgcgg ctcgaatgca aaccgttaat ccaagcgttc
     2581 atccaccgca ccgttcgacg atccccagcg gccgagccga aggccttctt aggccaaaac
     2641 cgcagcaggc ggaactgtgt gtttggtcgc acagaaagct gaggcagaaa gccggggctt
     2701 tgcttttgca gcgcgcctcg ctagattgag cgccatgcaa tcgctggccg caacgggatt
     2761 cgcgcgccgc actcgtcgcc tctggccggc gttggcggcg atcgcgacgt gtttatgcct
     2821 tgccgtgtca gcccgtgcag gcgatgtgcc gcttatcggg accatcgagt ggcgggcaag
     2881 cgacaacttg gtcgatgcgc aaatcgaagg ctggccgctg cagaaggttc tcgagcagat
     2941 cgccgcccga acatcctggc atgtctacgc cgaaccaggc gcccaacatg tcatctccgc
     3001 caaattcaaa aacctgaagc cgggcgacgc tctgcgccgg ctcctcggcg gtctcaactt
     3061 cgcgctgatc ccgtccaaca gcggtccggc gcgcctctat gtgtttctca gctcgcaggc
     3121 cggcgccacc gtgcggatcg aggccccgga ccaggccagg acggaggggc aggccaaacg
     3181 ctccgtgccc aacgaattgt tggtgacgct caagccgggc gccaaggagt ccattgacga
     3241 cctcgccaaa cggctcggcg cgcgcgtggt tggtcgattg gatgccctgg gcgcctaccg
     3301 gctcgagttc gaagatagcg cggcaaccga cgccgcccgc gccgctctgg ccgagaacag
     3361 cgacgtcagc gccatcgaag agaatctcgg cttcgcgcgg cccgaggttc cccaggtcca
     3421 ggcctccggc accgcgcccc cgctggttct gcgcccgcgg gccgtcactg acggcagcca
     3481 ggtcgtcatc ggcctgatcg atacgcctgt ccaacagcaa gggacaattc tcaaggactt
     3541 tctcctgccc ggggtctccg tcgccgggga ttcggcaatc gccgccgaca cgctcagtca
     3601 tgggagtgcg atggcgacga cgatccttta cggtctggcc cgttcctccg gcgcctcgat
     3661 cgacacaccg gtccgcatcc tccctgtcga cgtctacggc gcttcgggca gcaccaccac
     3721 ctttcaactg gcggagggaa ttctcgaggc gatcaaccgc ggggcctcga tcatcaacct
     3781 gagcctcggc ggcgaaaccc aaagcccgct tctcgaaagc gtgatccagg cgggtcatgc
     3841 gcagggcgcc gtgttcctgg ccgccacggg gaaccagccg gtgacaacac ccacctatcc
     3901 cgccgcttac tcacaggtca tcgccgtgac ggcgttggga caacacgggg agatcgcctc
     3961 ctacgccaac cggggcgact ttgtcgatgc cgccgctccc ggcatcagcg tcgttccgtt
     4021 tcagggccag tcctatgtcg tcgtcggcac ctcgacagcc accgccaatg cctcggccta
     4081 cgcggcccac gtcatggcga cgaccggcac gcgtggaagc caactcgacg ccctggtacg
     4141 tcaatcgctc ggagtcaccc caaccgcagg caaaccctaa cccgcataac agtccacttg
     4201 cgaagtccga ccgatccgtg cactcacggc gtatgaaaca tgcgcgtgac gcggactccg
     4261 acgaagcgcg tttgcgggag aacgaacttc ctaacctcga ccgtcgcggt cgtcgcgccg
     4321 aattcgcgca ggagcagatc ggccatctcg acggccaggg tttcgatcaa acaccaactg
     4381 cggcccgccc cccattcggc gagccgctgg cagaccgcgg cgtagtcgat actcgatcgc
     4441 aggtcatcct tctcggccgc gtcggcgaaa tcgtgggcga tttcaatcga gacgaggagg
     4501 cgctgcggct tggcgcgctc gtcctcggac acgcccaccc gcagaaacaa ctcgaggtcc
     4561 cggatgatga tcgtgtccat cactgcggcc cggcctgagc gaggtaggtc gtcagcagag
     4621 ttcgcgtcgg ctggttgagc ggaaggtcca tcgccgccgg cggcgtcacc cagcggtact
     4681 cctgcgcctc cgcgttgagg cgcaccaccg ccggccccgc gcaccggcac gtgtagttca
     4741 ggaggagaaa atgcgcgtcg cgatagaact cgcgcgagtg aatgcagtct tggaccatga
     4801 cgaaggcgat gtcgcggacc tccaaatcgg tctcctcctt caactcgcgc cgcagcgcat
     4861 ccgcacaccg ctccccgagt ttgatcttgc cgccgggaat cccccaaaga ttcgaccatt
     4921 tgcgcgtgcg caccatgagg acctcgccgg cctggttgaa gaccagagcc ccgaccgtgg
     4981 ccacggggcc ggcgggcgtg cgttttcggg cggcgggcgc cgccccttcg atccggaatc
     5041 cctggagttc gagcagatcg cgcagttcgc cgaggtgctc gacgatcaga tcgggctggc
     5101 ccgcccgcaa ttgagccagg ttgttgtaac cggtgagcac ggcgcacgcg tacacaccgc
     5161 cgcaccgggc cgtttcgaca tcatgttcca tgtcgccgat gaacacggtc tggcgcggag
     5221 ccaggccgtg gtctcccagc aactggtgaa tcttcgcccg cttgtcccac acttccacat
     5281 acgggtggtc caggtacctc ccaaatccgg tctccgccgc ctgagccgcg aagtggtccc
     5341 ggtgcacgct gctgagcagg aaggtccgca gcccatgccg gcggcagaac tcgagaaact
     5401 cgcgggcgtg cggcaactcg cgaaccaaat gttgcgactg acgaaaggcg ctgtggaacc
     5461 attcttcgag ttgaggccag ggcacgtgcg gcacgtagcg gtcgtagaat gtcttgaacg
     5521 gcaggcagaa ctcggaccgg aattgctcga gcgtcatctc cggaaccccg gcctgacgga
     5581 acacgtggtt gctcgccgcc aacaccgccg gcagatcgtc caccaaggtc cccgaccagt
     5641 cgaagatgac gttccgaatc acagcgacaa gcctacgcct ggcgcgcacc gcaacgcaat
     5701 cgcgatcggg catcgcgact tcgttccgtc tgccgggaat gaccggggac gcaggccccc
     5761 ggtcatctcc gcagcacggt tatcgagcgc cggctatttc cgcagccgga agaactgcgc
     5821 gttcgcgctg ggctcgatgt aggccgcgag ctcggccccc tcagccacaa ccggggccgt
     5881 ggaatccgcc cagccgcccg gcagcgcgcc tgtcacctgc aggctgaatc cgatggctgt
     5941 tgccggccag ctcacgcgca cggtgcccgt ggacgtcatc ccaaccttga gagacgcctt
     6001 caccggcagt gtgccgcccg gcgtcacctt gacatcatcg agcagcagca cctggtcgcc
     6061 gccggcgacc tgttcgagcc ggatgaccgc ctccgtcccg gcggcctgaa acacagccgt
     6121 cttcaccgca tacggctggc tccccccgac gggcgtgatg gcttcggtcg ccgcctcctc
     6181 gccatccgcg ctgatcttca ggatcggcgt ggtgccggtc gaggcattga ccgcgacgct
     6241 gacggtgtag ttctggcctg gggtgaggcc ggtgattgtc tggctcagcc aggcggcgcc
     6301 ttgcaggaag gcgacgttgt cctggtcggg attggcgccg ttatcggcga agccgagccc
     6361 gctggtgttg atgccccggt tgcctgcgcc gccggcggtc cagccggcga tgttcttggt
     6421 gatcgtgccc ggcaggggca cgacgccgct ggcctcgaaa ctcgggttga cgaccgggat
     6481 gttgtccggg tcgcgctgga cgatggtgat ggcatcgaga agcacagtgg catccccgga
     6541 ggctgtggtt tcgaacgcca gcaggcccga ggcggccgcg ggcgtgaatt cgtactgctg
     6601 gaagtaatag gacggcccat cggggagatt ggacccgccg accggcgtga tggcggcaat
     6661 ggtgcccaga gggaggtcat cgaagctggc cgtcaggtcg atcgtgcctc cgcagcagtt
     6721 gcgcacattg tagtagaact gcagccagta cgttttgcct ggcgtgaggt tcatgatgtt
     6781 ctgacgaatg atcttcgacg tctgaagcag cgccaccagg tcacgatcgg gcgttgcccc
     6841 gttgtccgag aacggcccat ccgcctgcac cgggttgatg ccggtgttgc cgctcccttc
     6901 gctcggccag ctctggatcg ccatgtaacc gggatagccc ggcacctcgt ccgcctcgaa
     6961 gctcgggttg cgcacaaacg acccgagcac cagcaccgac acctctttcc ggttgaagca
     7021 gacgccgtgc ggattggcga attgcagggc agtcacaccc ttggatactc ccgtcaccgt
     7081 caccgtcgcc gacaaggctt ccggatcccc gcccaccggg aattcgagcg tgagcacgcc
     7141 gcccacggct ccctcgaccc gggccacggc agggttcaga ctgctgaccg tgacccgcgc
     7201 cggatagctc aactgcagga gctcgccagg cacgctgacg gtgaggccgg ccgaatccgt
     7261 ctcgcccacc gccagttgga gcgcggcggg ccggacttcg atgcacggga cgttgatgga
     7321 caccccggtc accttgacgt cgtcgagcag aacggtctgg gtggggtccg cgaccacctg
     7381 ttcgaaccgc aagaccgccg tgtccgacgc cgccgtgaac gaggtcgagc caacccagta
     7441 ggccgccgag ccgcccacag gcgtcacatc cacttcgagg agcgacgcgc cgtcggcgct
     7501 gaccctcagg cggggcgcct ggcccaaggg ggcattgtag gcaaaggtga ctttgtagtt
     7561 ctggccgggc aacagaccga ggatggtctg actcagagcg cccgggccgc ggataaaggc
     7621 caccaggtcc tggtcgggat tggccccgtt gttcgcgtac acgcccaggc ctgagaagtt
     7681 gatgcctgtc gtcccctcgg tggtccagcc ggcgattctg gccagcacac cctccccggc
     7741 gagttcgccg ctcgcttcga agctcgggtt ccgcaccacc acgttgccgg cgtcgcgctg
     7801 tacaatggtg accgcgtcaa acacgagcga ggcgtcgccg ctatggacag tgtgcacgat
     7861 ctcgaacagg cccgaagcgc tcgagggcgt gaacgcgaag ttccggaatc catacggatt
     7921 ctgcccgccc acaggcacga cgcccttgac actgccgatc tcgacccctt cgaactgaag
     7981 ggtcatgtcg tcgagcacgt cgccgcagca gttgcgccga ttgtaccaaa tctgcgccca
     8041 gtactcctta ccgggttcga gtccggagat ggtctgcgaa acactgccgc tgccttgttt
     8101 gaacccgacg cgattgccgt ccgggattgt gccgttgtcc gcgaacggtc ccgtcgcgcc
     8161 ctggttgacg ccgctgccgc cgtcccagtt gtcgatcgcg ctgtacgacg gccaggtcgc
     8221 gttgtagttg ctctcgaaac tcgggttgcg cacgaaggcg ccgctcacct tgaccgaaat
     8281 cgccgttggg aagttgcagg cgcccgcgct attggcgatc tggagagtcg tgatgccgcc
     8341 cccgacaagg ttgagatcga acgtctgctc gctcggcccg cccacgggga acatcagcgt
     8401 caacgcgccc ccagccccgc ttgccggcgt cgccaccctc ggatcggccg aggtcaccgt
     8461 caccgtcacc ggcttcgtcg cggtcaggtg cgcggaaacg cggatcgtca ccgtcgccgt
     8521 gccgccgacg gtgccgctga tgagcgaggg cgatccggcc acacagggct cggcggcggc
     8581 gttgtcggaa tagatgcaca agttgtcgaa tatggccgtc accatgtcgt ccggggcgcg
     8641 tgtataggcg ccaaactcaa acacgatgcc gtcgctgacc gggaagggca cgtccacgcc
     8701 catcaggccg tccaggaaca gcttcaccgt cgcgccgtcc gcaaccatct tcatccgatg
     8761 cagcccgcca tcctcgaagg ggagatagtc gaacgcgggg atatcgattc cgtttcctgt
     8821 ggcggagtcg ccggccaatc cgatcttgcg gttgtattcc cagcctcctt cacccgtgtt
     8881 gtaggagaaa aacacgtagc gcgagcgggt ctgatccgtg atccagacgc cggatcgcgt
     8941 cgaggttccc gagagtgttc cgtagctctc gtggaggacg cgatcgatct caagtgtcag
     9001 cggcgcctca ggcgaagccg agaaggtctt ctccgtcgcc agggactggc cgccccagta
     9061 ggagccgtcg gcataaaggc tgaactcgat tcccgtcgcg ggattgacca tcacctgacc
     9121 gaacgccgat ccggtctcga acggggcgag gttcagacga tagaacggat cgaatggttc
     9181 gctctggaac tcgtcgcacc agactgccgg agacgccggg tagaccgtca cgcgcaccgg
     9241 gtcgcccacg catacgcccg cgccgttgct gagcgcgatc tccgtcgttc cccgtccgac
     9301 accgacgaga ttcaccgtct tggtcggcgc ggcgcccgcc gcgaacgtga cctcgaggct
     9361 gcccccaacg gcgcccgccg gcacggccac cgccgggtcg gcgctggtga gggtgactgt
     9421 agccggcgcg gaggcgttca tcgacttcgg aatccgcacg gtcacggcct gatcgttgcc
     9481 gccgactttg gcgaagaccc tcgaaggcga cgccgtgacg cacggctcgc ccgagatttg
     9541 gaagttgtcg aacgtgtcga tccacgtctg ggcggcgtcc gcgtagttca ggagagtgat
     9601 gtaattggca agaaacccgt tcgtattcac gagcaccgtc gggctcgatg gatcgctggt
     9661 gatggtcagc ggctggccgt tgaccgagac gctgatggcc acgggcgtgt tcgtgccccg
     9721 aaagccgtcg ctcgacacat cgatcttcac gtgaaaagcc tcgcccgtag gcagcgtcgt
     9781 ccccgccggc gagccgtaga ccgccgcggt gcggttgaac acctgccagg cgccgttgtg
     9841 ccgaaacagg ataccgaatc cgtcggacgc gttcacgaac atgttgcggg cggtggcgcc
     9901 caaaacgatc gccgcccagt cgcccgaact cgagccgagg tcgttcacgc ccggatcgag
     9961 atcgaactcg acgctgaaat ggccgccctc gataaagctg tgattggggc tgatcgatgt
    10021 ccacccgccg ttgacggccg tcagagcgag tgtgccggga tactcggctg tgttgaccgc
    10081 ggtgcggtcg tcataagtgc cattggcggc cgtgtccggg acttcgacat agggcagggc
    10141 gccgacggac ccgctcacgc gggtgttgat ctgatagttg acgtcccacg tcggctctgt
    10201 ggtgttgaag ttgtccagcc acagaacttc cgacgcggcc atcgccgtgc acaggccagc
    10261 caggccgccc aaagcagccg cccggccaac caccgaacgc accgttcgga aattgaatga
    10321 gagacagttc atggttttat cagggatttg gttgatgttt tcagcccatc gccaagccgc
    10381 gtccgcactt gctgctgcag gcgcgtttag tacggtattc gccccaaatg aaaggccctc
    10441 gaccgtcgga atgcaacggc aaaagttcgc aaaacgtagg gcgaaatgcc gatcctgtca
    10501 tgcccgttgc gcgccgcgct tgaatccgac cggcgagacg cgctagcctc tcgcgatgga
    10561 acacgggtcg gtcctcgctc caagtccatg gatgatattg ccctttggcg cgctgttggc
    10621 ggccattgcg ctggcgccat tggtttgcgc gaactggtgg gcacgtcatt atcccaaagt
    10681 cgctttgggc cttggcgccg tgacgctggg ctactacctg ttctggattc acgcccacga
    10741 gcgcgtcgag cacacggcga tcgagtacgt gagtttcatc gtcctgatcg gggctctctt
    10801 cgtcgtctcc ggcggcattc acatcacggt gcaaggcgaa gccaccccgc gggcgaactg
    10861 cgtgtttctt ctgattggcg cgatcatctc caatgcgctg ggcaccaccg gcgcctcgat
    10921 gctcctgatc cgcccctggc tgcggatgaa cgcgtatcgc atcacggccc accatgtggt
    10981 cttcttcatt ctgatcgttt cgaacgtcgg cgggtgcctg acccccatcg gcgatccgcc
    11041 gctcttcctc ggctatctcc gaggcatccc gttctggtgg gtggcacagc attgctggcc
    11101 gatgtgggcg gtcggagtcg gcgccctgct gctcctgttc tatgtcgtgg atcgatgcaa
    11161 ctacgtccgg gcgccccgcg aagtccgtaa gaagttggcc gaaccgcctg accgctggcg
    11221 tttcgagggg ctggtgaatc tcgtgttcct gggggcgatc ctgggcgctg tgttcatcaa
    11281 tcgccccccc ttcctgcgcg aggccatcat gctggccgcc gccgtcgcct cttactacag
    11341 cacgcgcccg gcgatccacc aatcgaacca tttcaatttc catcccatcc gggaagtggc
    11401 gattctcttc atcggcatct tcgcgacgat gatgccggcg cttgactggc tcgagatgaa
    11461 tgccgcgcgt ttcggcgaac ctcgggcggg ccagttctac tgggcctgcg gcatgctctc
    11521 gagcgggctc gacaacgcgc ccacctatct cagtttcctt agcgcgtgcc tgggatcgct
    11581 cgtggacgcg gacgtggtcg aacaggtgcg cgccattctg gccgcgggcg gatcgctcgc
    11641 agccgtgccc gacggcccca atgccgaggc ggttcggcag actctcgaca ctctgaggcg
    11701 gttctacccg gtcgctgtgg ccgcgggatc agtctcgccc aatcaaatcg aaatggccgt
    11761 cctgctcggc gacccacacc tcaaccggtt catcatcgcc atcagcatcg gggcggtctt
    11821 cttcggcgca aacacctaca tcggcaacgg gcccaacttc atggtcaaga cgatcgccga
    11881 tcagcggcgg gtccgcaccc cgacattcct cggttacatc ttcagattca ctctcccgtt
    11941 tatgatgccg atggtcctcc tggtgtggct gctcttcttc cgcggatgac gccgtcgcct
    12001 ggcctgcgac accggctcga ttcatccatt tcaagtcgtt cgccgcgcag cccccgactc
    12061 ggcccggggc cggctcacac acgaacaaga cggttccgga acacacgcac ggtgcgatcg
    12121 gtgcacgcca catgcagatg cccattccag tcaaaggccg gccgcgcctg gatcgaacgc
    12181 ggcgcctcaa agaccgcgcg ccccgcgccg ttctggtcga ccgcatgcag ccggcccagt
    12241 ggatccccga cgaatgccgt gcccgccgca tcgcagaccg gaccggccaa aaggtaatcc
    12301 gcgcccgttg cgtacgagaa caacggcgcg cccccgactg caaaagcccg caccgcgccg
    12361 tccatgccgg ccaccacgac cgctccgtct ggccgcagcg ccggcgtagc cacggtgaac
    12421 cggggcatct gccgggtcca tagaacccgc tcatcgcgca gcgaccaggc gtgcagcgtg
    12481 gtctctcggt caccgttgac gcagaagaaa acaacgcccc gatcctcatc cacgacgggc
    12541 gccgccgcca ccaccatccg tgacgcgccg cgcttgccct ccggctgccg acacagaacc
    12601 gtttcccgcc catctcgatc gagccggacg caaaccacac cctcgccccg aacggcgcgc
    12661 atcgagt
//
LOCUS       DAOQRO010000055        12654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4092_length_12654_cov_5.299865, whole genome shotgun sequence.
ACCESSION   DAOQRO010000055 DAOQRO010000000
VERSION     DAOQRO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4092_length_12654_cov_5.299865"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKK20_03410"
     CDS             complement(<1..318)
                     /locus_tag="PKK20_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU98967.1"
                     /translation="MNTSLSRRAFLRHSAIATAGLTCWGGLGAGVPGANERIVLAFMG
                     TNGRGSDLARSFAKLPGVETAVVCDVDERAIAKGIQAASREQARAPRSEKDVRRVVEN
                     PDVD"
     gene            complement(467..691)
                     /locus_tag="PKK20_03415"
                     /pseudo
     CDS             complement(467..691)
                     /locus_tag="PKK20_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
     gene            846..1172
                     /locus_tag="PKK20_03420"
     CDS             846..1172
                     /locus_tag="PKK20_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98968.1"
                     /translation="MKTFGPIVLMSAAWLLAALASPRTRSAPVADPPAQTPSGTPPGT
                     GLVGHWQGSLKPAPMLELRLTLELTKGPAGDLAGVMVSLDQGGVRIPLTHVSERTRHR
                     DAASRS"
     gene            1223..2239
                     /gene="ruvB"
                     /locus_tag="PKK20_03425"
     CDS             1223..2239
                     /gene="ruvB"
                     /locus_tag="PKK20_03425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNU98969.1"
                     /translation="MADRLISGALSKPDAALEMTLRPSLFSEFAGQMKVRERIEIAVQ
                     AARQRKEPLDHILLNGPPGLGKTTLAHIIAKAMNAGLKCTSGPTIEKAGDLAGLLTNL
                     EEGDVLFIDEIHRLQKTIEEYLYPAMEDFKLDIIIDQGPNARSVRLNLPRFTLIGATT
                     RSGLLTAPLLTRFAVRERLDYYSAEQLQTIVLRSARLLSIDTDPDGATEIAQRSRGTP
                     RIANNLLRRVRDYAQVRGDGRITSAIADKALAMLEIDENGLDEMDKRILEAIIVKFSG
                     GPVGISSLAVAVGEEPDTLEEVYEPYLIMEGYLKRTPQGRVATELSYRKLGLKSPGAQ
                     PALL"
     gene            2353..4143
                     /locus_tag="PKK20_03430"
     CDS             2353..4143
                     /locus_tag="PKK20_03430"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNU98970.1"
                     /translation="MKTPNCLLPVGRLGRQRLPARRSARTAGLILAAALALAAGDLLA
                     QTPPAIGTVSALKPIPDADLQKIRSALPAQAIVPPQKPRKLLLFVRNVGYGGHPSAEY
                     ANHAFTLMGEKTGAFQAVVSRDPEVFRRDSLEPFDAVFFNNNVGNLFTDPELRRNLVE
                     FVTGGGGMLGLHGTTVAFTQWPGAAEDWPEFGQMIGARGANHREATEHVFIKLDDPHH
                     PLNAVFGGRGWEFRDEFFRVHDPYGRHRVRVLFSIDTEKTDLTKDRGAARLERADNDF
                     ALAWIRNYGRGRTFYCTIAHNPYVFWDPLMLQFYLGAIQFALGDLPAPTTPSAWLTPA
                     VRAQEKLGWRLGLEAYTFHRVTFFETIERTARLGLPYVGGLSFQKVSADIPKNFEPGL
                     SDDELCAIRLKLADAGLRMLTFYIQEIPGDEDGCRKVFEFGRKLGLETFMTEPKLESL
                     DTIERFANQYGINVALHNHDQKASPHYFSPDAILKVCAGRGPRIGAAADVGYWMRGGI
                     DPIAGIRQLGPRLLTLQMHDLDAITPQGVDVPWGTGQGRSEQIFREILRQGLKPTMIG
                     LEYSKDFDDNLSAVAQCAEFFNQLTLQLTR"
     gene            4140..4865
                     /locus_tag="PKK20_03435"
     CDS             4140..4865
                     /locus_tag="PKK20_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNU98971.1"
                     /translation="MSTPTATRVAAAALQQAFDALAQYNRGSGRDTLKPIDDAAMAAA
                     ADAGLRADIEARLIATLRSPASAVAKDYACGRLALIGGPEAVPALADLLPNTDLAHSA
                     TDALQRMTCDEAVRALRETLPKLAGLPLAGVITALGARRDLASVGALTALLSNTDALV
                     ASAAAAALGEIGSPAAATALREFLTKAPPAWTGVLADACLRCVTRLKASGELMEAHEL
                     ADALLSTGPASYVRDAFKALDLR"
     gene            5046..6467
                     /locus_tag="PKK20_03440"
     CDS             5046..6467
                     /locus_tag="PKK20_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNU98972.1"
                     /translation="MASLMTLALLCMLNAGAAEQATDPVRTETPAQRDERMSWWREAR
                     FGMFVHWGLYSGLAGTWEGKTVGTRGGMEWIQQRVKADTDAYAQAAIPKFKPGPDFAR
                     AWARLAREAGCRYVVLTSKHHEGFALHDSAASDYDAGSVLGRDLVKEFVDACRAEGLR
                     FGFYHSVIDWHHDQYAYGLSKQLPHPLQGKPYPNGERNHAVYLDFLHRQVDELVSNYG
                     AVDILWWDYSSQDFQGDAAWRAFDLMRKVRAKQPRIIMNNRLFRNPEAGWASMSTDGY
                     MPQLDPKYGDFITPEQHIPATGMPGVDWETCMTINTTWGYSEHDHAWKSPQILIRNLV
                     DIASKGGNYLLNIGPKGDGSVPRESIEAMRAIGVWMQKNGESIYGTQASPFEKLEWGR
                     CTRKTLANNITRLYLHVFDRPSDARLVLPLANRPLKAFLLDGGSILQAEAVGNSIVVP
                     LPAALPDAVVTVVALEIEGAPSL"
     gene            6515..7297
                     /locus_tag="PKK20_03445"
     CDS             6515..7297
                     /locus_tag="PKK20_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HNU98973.1"
                     /translation="MNRRHFLTVSGSVGLVTATGLDASAQETGAGRQYLVLQKYSFGA
                     EEQRKAFDAFMQDVALPALNRLGVQPVGVFLDPQEPRPVYVLLPHPDADSALNMNQRL
                     LADAEFARQGAAFIDAPKASAPYTEIESWLMLAFKAMPKVELPAKGPNRIFQLRTYES
                     PSLKTGQKKIEMFNDAGEIRIFRETGLAPVFFGETLFGGKMPNLTYMLGFEGEEQQKA
                     AWSAFVKHPDWRKISSMPEYADARVIRGITNTLLKPAPYSQV"
     gene            complement(7381..9741)
                     /locus_tag="PKK20_03450"
     CDS             complement(7381..9741)
                     /locus_tag="PKK20_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNU98974.1"
                     /translation="MTAALLVLAILALATVLLVTEWVAMEVTALLVLGAVTVTGLVTP
                     REAVAGFSNPAVVTIWAVYFLSGGLSRTGVASRLGRHVLRLAGRSETRIVGVVMLSAG
                     VLSAFMNNIAVVALLLPVVMDIARRTGQPPSRLLMPLALGSLLGGLTTLLGTATNLLV
                     SELMHDQAGVPFRLFDFTPVGMSVMLAGVAFVVFIGRRLLPRRDISDASAATPAASLL
                     AQYQLGERLFRLRVPAESPVADKTLGEIQINHVLDVQVVGIRRSASSELAPGPRTALR
                     VGDVLLVRGRQERLQELAGWRLLADDDETPVLEVLLAESFELAEIQVADNTDLAGKSV
                     RQIGFHERFGATLLGLRRGSITVESRLTEEVLQPGDTLLVHGPRPAIHKLSRDPQFRK
                     APGASAEKLVADYNLVQRLLVLRLPSDSNLAGQDLLSAGLGDTTELRILAALRSDGVV
                     LPCGPGLKLAEGDRLLALGTRPQLELVRALAALMFDSTPPDVDAALETEQAGLLEAVL
                     APRSGLAGKRVREAQLRERFGVSLLALWRGGRSYRSQLRAMDLRHGDALLLYGPRERL
                     QALGREPDFVVLTEAAQELPRARKAPLALAILGAVILAALLGWLPIYIAAVLGAAMMV
                     ATGCLRMDEAYRFIEWKAVFLTAGLLPLGLAANNSGAALWLGENLAGWTAPLGPVGIL
                     AALMGVTFAASAVLPPTAVVVMLAPVVMHMAAQLQLSPRALMMGMAVAAASTFINPLG
                     RAASLMVMGPGGYRYVDYVKVGLPLTLVVWLVTVGVLPLVWPLSAR"
     gene            10233..11198
                     /locus_tag="PKK20_03455"
     CDS             10233..11198
                     /locus_tag="PKK20_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU98975.1"
                     /translation="MQVFNGWVMLPINLILSLGGPTLLIYSIVSGANRYGHPVWGLFV
                     LALLMLITSFIMLFGFFALQPNEARVLVLFGEYRGTVRESGFHWGNPFYSNGRSASGA
                     TALAAAGKIAEAGAAAAAIKKQSRNKISLRARNLNGERLKVNDKRGNPIEIAAVVVWR
                     VRDTAQAMFDVDDYEEYVSVQSESAVRHLASQYAYDHGEDNEVTLRSGFDEVSQALTT
                     ELQERLDKAGVRVEEARLTHLAYAPEIAQAMLRRQQAEAVIAARQKIVQGAVSMVEMA
                     LHDLAEKHVVQLDDERRAAMVSNLMVVLCGESEVHPVVNTGTLYA"
     gene            11205..11426
                     /locus_tag="PKK20_03460"
     CDS             11205..11426
                     /locus_tag="PKK20_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98976.1"
                     /translation="MAERKSFLLRMDPALWAELEAWAQAELRSVNGQIEYLLRQAVQR
                     RRGAVAPLALAARASPGPVSDSGTQNSEL"
     gene            11423..11923
                     /locus_tag="PKK20_03465"
     CDS             11423..11923
                     /locus_tag="PKK20_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010540839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNU98977.1"
                     /translation="MNRYLLYAVIWSALVLGAVADLAVHYPRLPEKVATHFNESGRPD
                     GWSTRRSYVWTWSCGVGLLPAIILGTIAAVKYLPARWINMPHREFWLAPAQIEFTRAI
                     VAGMVHWIGVATFLFTIVLNHLTLRANLTPEPSLGVWPWILLGALFTAMAVIIVPVML
                     RFRRPR"
     gene            complement(11883..>12654)
                     /locus_tag="PKK20_03470"
     CDS             complement(11883..>12654)
                     /locus_tag="PKK20_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNU98978.1"
                     /translation="TRMKELTNEVPKPMLRVEGRPILEHILGGLIGAGIRDICIVTGW
                     HAEAIEGYFGDGGRWNCQIRYVRQVVQDGTGRAAEPARAFVGTDDFLLTYGDILVHPE
                     TYQRMLARFREAPFDGLLTVTAGEDVTKGAINFFDSTFCLTRLIEKPSLAQVEQLRRD
                     GWLKPGDPAWYNAGIYIFRPLVFEYTARLEKSPRGEYELTDAISAMVARRHRIAGLRI
                     AGRWVDVRDPEILSALERQSTAQPAVTLDAESAASPAR"
BASE COUNT         2354 a         4228 c         3933 g         2139 t
ORIGIN      
        1 gtccacatcc ggattctcga ccacgcggcg cacatccttt tcgctccgcg gagcgcgggc
       61 ctgctcgcgg cttgccgcct gaatgccctt ggcgatggcc cgctcgtcaa cgtcgcacac
      121 caccgccgtc tcgacccccg gcagcttcgc gaagctgcgc gccagatcgc tgccccgtcc
      181 gttggtcccc atgaaagcga gcacgatgcg ctcgttcgcc ccgggaaccc cggcccccag
      241 gccgccccaa catgtcagtc cggcggttgc aatggcggaa tggcggagaa acgcgcggcg
      301 agacaatgaa gtgttcatag ggcgatgcat aggtcgatat ccaaggtcaa cggtgctggc
      361 ggtttgcggg cgtgcgcgcc ctcacacttc tgcattgtga tcgcgcgcgg ttcatgggct
      421 ttcggatcgc gtgggaggag actctgcttc actcgatcgc ggccgagtcc gccccctgtc
      481 ccgtgccctt cttgatggcg cgttcgatgg tgtcgcgcgt cactgaactc ttgcgcgcct
      541 tctcgaccgc cgccgccagg cggggattca gatgcggatc gcccccgccc atcttcgccg
      601 cgacaatcaa ctcgcgcacc agtttgttga caagctggcc cttcttctgc gccgcggcct
      661 cgcgtccggc ctgcttccat tgtgctccca taaaacgaac gctgtctgcc ttgtgtcgca
      721 aacaaggtta gccgaaagac cggacgaccg gaagcatttc ctgatccggc tgctcaatgt
      781 gttgtggtct cgggaatacc tgcgtatcat tgctcccgag caatcgacat tcactcgttc
      841 gcgatatgaa gacctttggc ccaatcgtcc tgatgtccgc cgcctggcta ttggccgcgc
      901 tggcgtcgcc ccgaacccga tccgcgcccg ttgccgatcc cccggcccag acgccctctg
      961 gcacgccgcc gggcacggga ctcgtcggac actggcaggg atcgctcaag cccgcgccga
     1021 tgctcgagtt gcggctgact ctcgaactga ccaaaggacc cgcgggcgat ctggcgggtg
     1081 tcatggtgag cctcgatcag ggcggtgtgc ggattcccct cacgcacgtg agcgagcgca
     1141 cacggcatcg agatgccgca tcgcgcagtt gaccgttttc cccttgtccc tcccgcgggc
     1201 tttctccatg ctcgcccgca cgatggctga tcggctcatt tcaggggctt taagcaagcc
     1261 ggacgccgcg ctcgagatga cgctgcggcc ctcgctgttt tcggagttcg ccggccagat
     1321 gaaggtgcgc gagcgcatcg agatcgccgt ccaggcggcc cgccagcgca aggaacctct
     1381 cgaccacatt ctcctcaacg gcccgcccgg cctgggcaag acaaccctgg cgcacatcat
     1441 cgccaaagcc atgaacgcgg gcctcaagtg caccagcggc ccgacgatcg agaaggcggg
     1501 cgatttggcc ggcctcctca cgaacctcga ggaaggcgac gtgctgttca tcgatgagat
     1561 ccaccggctg cagaaaacga tcgaggagta cctctacccc gccatggagg acttcaaact
     1621 cgatatcatc atcgaccagg gccctaacgc ccgcagcgtc cgcctcaacc tgccgcgctt
     1681 cacgctcatc ggtgccacaa cacgcagcgg actgctgacg gcgccgctgc tcacccggtt
     1741 cgccgtgcgc gaacgcctcg attactacag cgccgaacaa ctccaaacca tcgtcctccg
     1801 ctccgcccgc cttctgtcga tcgacacgga tccggatggg gcgaccgaga tcgcccagcg
     1861 cagccgcggc acgccgcgca tcgcaaataa cctgctccga cgcgtccgcg attatgccca
     1921 agtccggggc gacggccgaa tcacgtccgc catcgccgac aaggcgctgg ccatgctcga
     1981 gatcgacgaa aacggcctcg acgaaatgga caagcgtatt ctcgaagcga tcatcgtcaa
     2041 gttcagcggc ggacccgtcg gcatcagttc gctcgccgtg gccgtcggcg aggaacccga
     2101 caccctcgaa gaggtgtacg agccttacct gatcatggaa ggttacctca aacgcactcc
     2161 ccaggggcgc gtcgccacag agttgagcta ccgcaaactg ggcctcaaaa gccccggcgc
     2221 ccagcccgct ctgctttagc gcggatcgtt cgtagcacgg cactcaccat gcgaagtctg
     2281 accgcccggt gcaccgccgg gggcgaattc ctttgccgtt tcggggcggt ctctgcaaaa
     2341 ttgtgctccg gaatgaaaac gcccaattgc cttctccctg tgggccgact cggacgccag
     2401 agactgcccg cccgcagatc ggcacggaca gcagggttga tcctggccgc cgcgcttgcc
     2461 ctggccgccg gagacctcct ggcccagact cccccagcca tcggcacagt ctcggccctg
     2521 aaaccgattc ccgatgccga tctgcaaaag atccggtcgg ccctccctgc gcaggcaatc
     2581 gtcccgcccc agaaaccgcg caagctcctg ctcttcgtcc gcaacgtcgg gtacggcggg
     2641 catccctcag ccgagtacgc caaccacgcc ttcacgctca tgggcgagaa aacgggcgcg
     2701 ttccaggccg tcgtgagccg cgacccggag gtgttccggc gcgacagcct cgagccgttc
     2761 gacgcggtct tcttcaacaa taacgtcggc aacctgttca ccgatcccga actccgacgc
     2821 aacctggtcg agttcgtgac cggcggcggc ggcatgctcg gcctgcacgg cacgaccgtc
     2881 gccttcacgc aatggccggg cgcagccgaa gactggcccg agttcggtca gatgatcggc
     2941 gcgcgcggag caaaccatcg cgaggccacc gaacacgtgt ttatcaagct cgacgacccg
     3001 catcatccgt tgaacgccgt cttcggcggc aggggctggg agtttcggga cgagttcttt
     3061 cgtgtccacg acccctacgg acgccatcgc gtgcgcgtgc tgttcagcat cgacaccgag
     3121 aaaaccgacc tgaccaaaga ccgcggcgca gcccggctcg agcgcgcgga caacgacttt
     3181 gccctcgcct ggattcgcaa ctacgggcgg ggccggacgt tctactgcac catcgcccac
     3241 aacccgtacg tgttctggga tcccctaatg ctccagttct acctcggcgc gatccagttc
     3301 gccctgggcg atttgccggc ccccaccacc ccgagcgcat ggctcacccc cgcagtccgc
     3361 gcccaggaaa agctcggctg gcgcctcggc ctcgaagcct acacgttcca cagggtcact
     3421 ttctttgaga caatcgagcg gacggcgagg ctcgggttgc cgtacgtcgg cgggttgagc
     3481 ttccagaaag tcagcgccga tattcccaag aacttcgaac cgggcctcag cgatgacgaa
     3541 ttgtgcgcga tccggctcaa gctggccgac gccggcttgc ggatgctcac cttctacatc
     3601 caggagattc cgggcgacga agacggatgc cgcaaggtgt tcgagttcgg acgcaagctc
     3661 ggcctcgaga cattcatgac cgaaccgaaa ctcgagtcgc tcgacaccat tgagcggttc
     3721 gcgaaccagt acggcatcaa cgtggcgctg cacaaccacg accagaaagc ctcaccccat
     3781 tacttcagtc ccgacgccat cctcaaggtc tgcgccggac gcggcccgcg catcggcgcg
     3841 gccgctgacg tcggctactg gatgcggggc ggaatcgatc cgatcgccgg cattcgccaa
     3901 ctcggccccc ggttgttgac cctccaaatg cacgatctcg acgcaatcac gccccaaggc
     3961 gtcgacgtgc cctggggcac cggccaggga cgatccgaac agatctttcg cgaaatcctg
     4021 cgtcagggat tgaaaccgac gatgatcgga ctcgaatact cgaaggactt cgacgataac
     4081 ctctccgccg tggcccagtg cgccgaattc ttcaaccaac tcaccctgca actgacccga
     4141 tgagcactcc aaccgccacc cgcgtcgccg ccgccgcact ccagcaagcc ttcgatgccc
     4201 tggcccaata caaccgcggc tccggtcgcg acacgctcaa accgatcgac gacgccgcca
     4261 tggcggccgc agccgatgcc ggcctccgcg ccgacatcga ggcgcggctg atcgcaacgc
     4321 tccgcagccc ggcgtccgcc gtcgccaagg actacgcctg cggacgactg gctctcatcg
     4381 gcggtccaga agctgttccc gctctggccg acttgctgcc gaacaccgac ctcgcgcact
     4441 ccgccacaga tgccctccag cgcatgacat gtgacgaagc ggtgagagct ctgcgcgaga
     4501 ccctgcctaa gctcgccgga ctcccgctgg ccggcgtcat caccgccctg ggcgcgcgcc
     4561 gcgatctggc cagcgtgggc gcattgaccg ccctgctttc aaacaccgat gcgctcgtgg
     4621 catccgccgc ggccgctgcc ctgggcgaga tcggatctcc ggcggccgcg acggcgctga
     4681 gggagttcct gaccaaagcg cctcccgcat ggactggggt gttggctgac gcttgcctac
     4741 gttgcgtcac gagactcaag gcctctggcg aactcatgga ggcgcatgaa ctcgccgacg
     4801 ccttgctcag caccggtccg gcgtcgtacg tgcgcgatgc gttcaaggcg ctcgatctgc
     4861 ggtaagggga cagccactcg ctcttcctcc agcatcccgg gcgccaattc atggagtgac
     4921 ccaataaaga caataaagat aaggtgacag gttctttatc atgaacctga aacgttcaac
     4981 gcctaaccag cggagccttg accgccggtt atggcaaaag caaattccgc ctcatgcgaa
     5041 cgcaaatggc atccttaatg actctggccc tgctgtgcat gttgaacgct ggcgccgcgg
     5101 agcaggcgac agacccggtc cggacggaga ctcccgccca acgcgacgaa cgcatgagct
     5161 ggtggcgcga ggcgcgcttt ggcatgtttg tgcactgggg tctctattcc ggcctggccg
     5221 gcacttggga gggaaaaacc gtcggcaccc ggggcggcat ggaatggatt cagcagcgcg
     5281 tgaaggctga cacagatgcc tatgcgcagg ccgcgatccc aaagtttaaa ccgggtccgg
     5341 acttcgcgcg cgcgtgggcg cggctggcca gggaagccgg atgccggtac gtggttctga
     5401 cgagcaaaca ccacgagggt tttgccctgc atgactcggc ggcaagcgat tacgacgcgg
     5461 ggtcggtgct ggggcgcgat ctggtgaagg agtttgtcga cgcttgtcgc gccgaggggt
     5521 tgaggttcgg tttttatcac tcggtgatcg actggcacca cgaccagtac gcctacgggc
     5581 tctcgaagca gctcccgcat ccgctccagg gcaagccgta cccgaacggc gagcgcaacc
     5641 acgcggttta cctcgacttt ctgcaccgcc aggtcgacga gttggtctcg aattacggtg
     5701 ctgtggacat tctctggtgg gactacagct cgcaggattt ccagggcgac gcggcgtggc
     5761 gcgcctttga tctgatgcgc aaggtccgcg ccaagcagcc tcggatcatc atgaacaacc
     5821 ggctgttccg gaatccggag gcgggctggg cgagcatgag cacggacggc tacatgcccc
     5881 aactggatcc gaagtacggc gatttcatca cgccggaaca gcacattccg gcgacgggga
     5941 tgccgggcgt ggactgggaa acgtgcatga cgatcaatac gacgtggggc tacagcgaac
     6001 acgatcacgc ctggaaatcc ccccaaattc tgatccgcaa cctcgttgac atcgccagca
     6061 aaggaggcaa ctacctgctc aacatcggcc ccaaaggcga tggcagcgtc ccgcgggaaa
     6121 gcatcgaagc catgcgcgcc atcggggtct ggatgcagaa gaacggggag tcgatctacg
     6181 gcacccaggc cagcccgttc gagaaactcg agtggggccg gtgcacccgg aagacgctcg
     6241 cgaacaacat cacccggctc tacctgcatg tctttgaccg accgtccgac gcccggctcg
     6301 tgctgcccct ggccaacaga cctctcaagg ccttcctgct cgatggcggc tcgatcctgc
     6361 aggcggaggc cgtcggcaac tccatcgtcg ttccgctgcc ggccgcgctc cctgacgcgg
     6421 tcgtcaccgt cgtcgcgctc gaaatcgagg gcgcgccgag cctttgactt gataccgggc
     6481 gcgagatcgg tattctcgcc ccgcacacgc atctatgaat cgacgtcatt ttctcacggt
     6541 gtccggatcg gtcggacttg tcaccgccac aggcctcgat gcttccgccc aggagacggg
     6601 agcaggccgc caatacctgg tgcttcagaa gtactccttc ggcgccgagg aacagcggaa
     6661 ggcgttcgac gccttcatgc aggacgtcgc attgccggcc ttgaatcggc tcggcgtcca
     6721 gccggtgggc gtgttcctcg atccccagga gcccagaccg gtctatgtgt tgctgcccca
     6781 cccggacgcc gacagcgcgt tgaacatgaa ccagcggctg ctggcggacg ccgagttcgc
     6841 gcgccaaggc gccgcgttca tcgacgcccc gaaggcgtca gccccctaca cggagatcga
     6901 gagctggctg atgctcgcgt tcaaggccat gcccaaggtc gaattgccgg ccaaggggcc
     6961 caaccgcata ttccaattgc gcacctacga aagtcccagt ctcaagaccg gccagaagaa
     7021 gatcgagatg ttcaacgacg ccggtgaaat ccggatcttc cgcgagaccg ggctggcgcc
     7081 ggtgtttttt ggcgagacct tgtttggcgg caaaatgccc aacctgacct acatgctcgg
     7141 cttcgagggc gaggagcagc agaaggccgc atggagcgcg ttcgtgaagc atccggactg
     7201 gcggaagatc agcagcatgc ctgagtacgc cgacgcccgg gtgattcgcg gcatcacgaa
     7261 caccctgctc aagccggccc cctactcgca ggtctgaacc cggcgcggct tctccgcggt
     7321 cgaggcctgg actgttcgag ctgaggcgtg gcagtggcgg ctatgcgacg cgagctgtat
     7381 ttaccgggcg gacagaggcc agaccagcgg cagcacgccc acggtcacca gccataccac
     7441 cagcgtcagc ggaagaccca ctttgacgta atcgacatac cgatagccgc ccggccccat
     7501 caccatgagg ctggccgccc gccccagcgg gttgatgaat gtggaagccg ccgcaaccgc
     7561 catccccatc atcaaggcgc gcggcgagag ctgcagttgc gcggccatgt gcatcaccac
     7621 aggcgcaagc atcaccacca cagccgtcgg cggcagcacc gcgctggcgg cgaacgtgac
     7681 gcccatgagc gccgccagga ttccgaccgg tccgagcggg gccgtccagc ctgccagatt
     7741 ctccccaagc cagagagcgg cgccggagtt gtttgcggcg aggccgagcg gcagcaggcc
     7801 ggcagtcaag aacacggcct tccactcgat gaaccggtag gcctcgtcca tgcgcaggca
     7861 gccggtggcg accatcatgg cggcgcccag caccgcggca atatagatgg ggagccatcc
     7921 gagcagggcg gcaaggatca ccgcgcccag gatcgccagc gccagaggcg ccttgcgcgc
     7981 gcgcggcaat tcctgcgcgg cctcggtcag cacgacgaag tccggttcgc gcccgagggc
     8041 ctgcagtcgc tcgcgcggcc cgtagagcag cagcgcatcg ccgtgccgca ggtccatcgc
     8101 gcgcagttgt gaccggtaag accgtccgcc gcgccacagg gccaggaggc tgacgccgaa
     8161 acgctcccgg agttgcgcct cgcgcacccg tttgcccgcc agccccgatc gcggggcgag
     8221 gacggcttcc aacagacccg cctgctcggt ctccaatgcc gcgtcgacat ccggcggagt
     8281 ggagtcgaac atcaatgcgg ccagcgcgcg cacgagttcg agttgagggc gggtcccgag
     8341 cgcgagcaaa cggtcgccct cggccagttt caagcccggt ccgcacggca gcaccacgcc
     8401 atccgagcgg agggcggcca ggatgcgcaa ctccgttgtg tcgcccaggc cggcggatag
     8461 gaggtcctgg cccgccaggt ttgaatccga gggcagacgc aacaccagca ggcgctggac
     8521 caggttgtaa tcggcaacga gcttctcggc ggacgcgcca ggcgcttttc ggaactgcgg
     8581 gtcgcgactg agcttatgga tggccgggcg cggtccatgc accagcaggg tgtccccggg
     8641 ttggagcacc tcctccgtca ggcggctttc gacggtgatg ctcccgcgtc gcaggccgag
     8701 gagcgtcgcg ccgaaacgtt cgtgaaagcc gatttgccgg acgctcttgc ccgcaagatc
     8761 ggtgttgtca gccacctgaa tctccgccaa ctcaaacgac tcggccagca acacctcgag
     8821 gacaggcgtc tcgtcatcgt cggccagaag ccgccacccg gcaagctcct gcaaccgctc
     8881 ctgccgcccc cgcacaagga gcacatcgcc aacccgaagg gcggttcttg ggcccggcgc
     8941 caattcgctc gacgcgcttc gccggattcc aaccacctgc acgtcgagca catggttgat
     9001 ctggatttct cccagggtct tgtccgccac cggcgactcg gcaggcaccc gcaggcgaaa
     9061 caaccgctcg ccgagctggt attgcgcaag gagactcgcc gccggcgtag ccgcagaggc
     9121 gtcgctgatg tcgcgccgcg gcagcaaccg gcgtccgatg aaaacgacga aggctacccc
     9181 tgccagcatc acactcatac ccacgggagt gaaatcgaac aggcgaaaag gcacaccagc
     9241 ctggtcgtgc atcagttcgg acacgagcag gttggtggcg gtgcccagca gcgtggtgag
     9301 tccgcccaac aacgagccca gcgccagggg catcaggagg cgcgagggcg gctggccggt
     9361 ccggcgcgct atatccatca ccacgggaag cagcagcgcc accacggcga tgttgttcat
     9421 gaaagccgac agcacgcccg cgctgagcat caccaccccc acaatccgcg tttccgaccg
     9481 accggcgagc cggagcacat ggcggccgag ccggctggcc acacccgtcc ggctcaagcc
     9541 cccgctgaga aaatacacgg cccagatcgt gacgaccgcg ggattgctga agccggccac
     9601 cgcctcccgc ggcgtcacaa ggcccgtgac cgtcaccgcg cccagcacca gcagtgcagt
     9661 cacctccatc gccacccatt cggtgaccaa cagtacggtg gccaatgcca gaatcgcaag
     9721 aaccaggagc gccgccgtca tttcgagagg aacgtgaaga tccccatagg gcaggcgtgc
     9781 ccagtccttc gatcagtcgt ctgtcggata agcatggctg tcgagcatga atggtcgatc
     9841 ctcctctatc aatgccgcaa atgcaaaacg gcgcgtttgc gcgtttggac gcccttcgtt
     9901 gcgtgatgga tgcatcgagt ggccatccgc caccggacaa caaacatctg gatgtccgcc
     9961 gctctttgat ttgattcatg gtggcagtca tgaaaacaga ctcatccagg acgcagggtc
    10021 tgcagattgc agccaaggcc aggaaagcag ccggcaaaat cagagctcag cggatcggtc
    10081 gacgaggtga cgaggtgaag agtatgcaca tttcacttgc atttcatttg gatcgctata
    10141 tgatattatc acgatagttt gttgcatgtt tcgaatctat aagtgggata gccatgaatg
    10201 ctgaacagca ctctgcgagt aaggaacagc ctgtgcaggt cttcaacggg tgggtgatgt
    10261 tgcccatcaa cctgatcctt tcgctgggcg gtccgaccct attgatctac tcgatcgtca
    10321 gcggcgcgaa ccgatacggc catccggtct ggggtctctt tgtgctcgcg ctcctgatgc
    10381 tgatcaccag tttcatcatg ctcttcgggt tcttcgcgct ccagcccaac gaggcgcgtg
    10441 tgttggttct gttcggcgaa taccggggga cggtccgcga gagcggcttt cactggggca
    10501 atccgtttta ctcgaacggg cggagcgcct cgggcgcgac tgcgctggcg gcggcgggca
    10561 agattgcgga ggcgggggcg gcggcggcgg ccatcaagaa gcaatcgcgg aacaagatct
    10621 cactgcgtgc gcgcaacctc aacggcgagc gactcaaagt gaacgacaag cgcggcaacc
    10681 cgatcgagat tgcggcagtg gtcgtgtggc gggtgaggga cacggcgcag gcgatgttcg
    10741 atgtggacga ctacgaggag tacgtcagcg tccagagcga atcggcggtg cgtcatctgg
    10801 cgagccaata cgcatacgat cacggtgaag acaacgaagt cactttgcgg agcggtttcg
    10861 acgaagtgtc gcaggccctc acaaccgagc ttcaggagcg cctggacaag gcgggggttc
    10921 gagtcgagga agcgcggttg acgcacctgg cctacgctcc ggagatagcg caggccatgc
    10981 tgcggcggca gcaggccgag gcggtgatcg cggcccggca gaagatcgtg caaggcgccg
    11041 tcagcatggt cgaaatggct ctgcacgatc tggcagagaa gcatgtggtg caattggatg
    11101 acgagcggcg ggccgccatg gtgagcaatc tcatggtggt gctttgcggc gagtccgagg
    11161 tgcatccggt tgtcaacacg gggaccctct acgcgtagac cggcatggcg gagcgcaaga
    11221 gtttcctgct gcgcatggac cctgcgctct gggctgaact cgaggcctgg gcgcaggcgg
    11281 aactgcgcag cgtcaacggc cagatcgagt acctgctgcg ccaggccgtt cagcggcgaa
    11341 ggggggcggt tgcgccgctt gctctggccg cgcgcgcatc ccccgggccc gtgtcggact
    11401 cgggaaccca gaactcggaa ttatgaatcg ctatctgctc tacgcggtga tctggtcggc
    11461 gctcgtgctg ggggcggtgg ccgatctggc ggtgcattac ccgcggcttc ccgagaaggt
    11521 ggcgacgcac ttcaacgagt cgggccggcc ggacggctgg tcaacgcgcc ggagttatgt
    11581 ctggacatgg tcgtgcgggg tcgggctcct gcccgcgatc attctgggaa cgatcgccgc
    11641 ggtgaaatac ctgcccgccc gctggatcaa catgccgcat cgcgagttct ggctggcgcc
    11701 cgcacagatc gagttcacgc gcgcgatcgt ggccggcatg gtccactgga tcggcgtcgc
    11761 cacgttcctc ttcacgattg tcctcaatca cctgaccctg cgcgccaacc tgacgcccga
    11821 gccaagcctc ggggtgtggc cctggattct gctgggcgcc ttgttcaccg cgatggcggt
    11881 gatcatcgtg ccggtgatgc tgcgctttcg gcgtccaagg tgaccgcggg ctgggctgtg
    11941 ctctgccgtt cgagagcgga caggatttcg gggtcgcgca catcgaccca ccgtccggca
    12001 atccgcaagc ctgcgatccg gtggcgacgc gcgaccatgg cgctgatggc gtccgtcagt
    12061 tcgtactcgc cgcggggcga tttctcgagc cgggcggtgt actcgaacac caacgggcgg
    12121 aagatgtaga ttccggcgtt gtaccaggcc gggtcgccgg gtttgagcca gccgtcgcgc
    12181 cgcaactgct cgacctgggc gagcgacggt ttctcgatca gccgggtcag gcagaatgtc
    12241 gaatcgaaga aattgatggc gcccttggtg acgtcctcgc cggcggtgac cgtgagcagc
    12301 ccgtcaaatg gggcttcgcg gaatcgagcc agcatccgct gataggtctc ggggtggacc
    12361 aggatgtcgc cgtaggtcaa cagaaaatca tccgtgccga cgaatgccct ggcgggctca
    12421 gcggccctgc cggtgccgtc ctgcacgacc tggcgcacat agcggatttg gcagttccat
    12481 cggcctccgt cgccgaaata cccttcgatc gcctcggcgt gccagccggt cacgatgcaa
    12541 atgtcgcgga tcccggcgcc gatgagcccc ccgagaatat gttcgaggat tggccggcct
    12601 tcgacccgga gcatgggctt ggggacctcg ttggtgagtt ccttcatccg ggtg
//
LOCUS       DAOQRO010000056        12512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4161_length_12512_cov_5.531669, whole genome shotgun sequence.
ACCESSION   DAOQRO010000056 DAOQRO010000000
VERSION     DAOQRO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4161_length_12512_cov_5.531669"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..824
                     /locus_tag="PKK20_03475"
     CDS             552..824
                     /locus_tag="PKK20_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNU98979.1"
                     /translation="MLCPKERYNEKPAPCFTRLQGRYLAFIHQYTTLHRVPPAEHEMQ
                     RFFQVSPPTVHQMILTLERKGLIKRTPGKARSIKVLVAAEQLERLT"
     gene            1046..1990
                     /locus_tag="PKK20_03480"
     CDS             1046..1990
                     /locus_tag="PKK20_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98980.1"
                     /translation="MNNDKLEYADLLELLINGPVGGPRRWAAWLVLRMLETKGNCCYR
                     SDDHLRGTERAQAAIASLRAAARALSVAEQGLLLRAILTLSWEQLRRNTGFSLRVPGI
                     LVELGKDLGVLPCLAAAESVVDQGNDAVRRYPLTGLLPEVRQPGSGLIFSLSARERKQ
                     LGVSADTIEFDRLTVAVKSFRGCGWEANLRPLVAWWIESLDHRDAARVVRGLFPDIRR
                     AAKDDQARWRALGLLDWLVDHPDHPTAGEAMTVAREVARADVRKAAADLAAALGQWEV
                     LEWLTGSDRDRSVRERAEKLLQGRVGPGASGGQDELFG"
     gene            complement(2013..2210)
                     /locus_tag="PKK20_03485"
                     /pseudo
     CDS             complement(2013..2210)
                     /locus_tag="PKK20_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2360..3793
                     /locus_tag="PKK20_03490"
     CDS             2360..3793
                     /locus_tag="PKK20_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU98981.1"
                     /translation="MRVLLVNTNRMKPAIAPLGLDYLADALEAAGHEPRLVDLCFSQD
                     IAADLEAAVRLGAPEVIGVTVRNTDDCYMSGQAFFLPEIKRAIQILRGSTTAPIILGG
                     VGFSVAPMAVMEFCGADFGMAGDAEFALVEFLRAIEQRRDWSAVPGLVYRDRGRWRLN
                     PVVPGDMARLPERRRALVDNARYFREGGQAGFETKRGCGMRCLYCADPVAKGRTTRCA
                     PPGLVVGELSALLAQNVDHFHTCDSEFNLPLEHARAVCQAIVDAGLGERIRWYAYCAP
                     TPFSDDLATLCRRAGCVGINFGVDSGCDSMLRRLGRHFTTADLLDTAATCRRHGLASM
                     YDLLLGGPGETRATVRQTIDLVRRAAPDCVGLSLGVRVYEGTPLAEWVRSQAEVPSHP
                     DLYGAKEAHPQLLAPVFYVAPGLAEDLVGWVHELVAGDSRFFLPTNTATHQNYNYNAN
                     ETLVRAIAQGARGAYWAILRRLRTAAG"
     gene            complement(4024..4608)
                     /locus_tag="PKK20_03495"
     CDS             complement(4024..4608)
                     /locus_tag="PKK20_03495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNU98982.1"
                     /translation="MLTGFMVAQSIVLADVKIGQPAPDFSGTDIQGKVHRLSDYRGNV
                     VVIEAYNLDCPFCANHYKSGAMQALQSSVVSKGVVWLVVNSTHPGNPSYRKPDAARKE
                     FAATGIKATAWIDDSSGAIGRKYGLKTTPHLVVINQQGLVAYQGAIDDRPASSGDPRT
                     ARNYVRQAIEALMAGKPVPTAETKPYGCSVKYAD"
     gene            complement(4741..6903)
                     /locus_tag="PKK20_03500"
     CDS             complement(4741..6903)
                     /locus_tag="PKK20_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNU98983.1"
                     /translation="MTSRSTVALAAALWLLATAATAAQPNTSARLVLSADTARAGEVV
                     WAGIHLTMAPDWHTYWRNPGESGLATSIEWHLPPGVSAGAIHWPLPESHTAAGMTTFI
                     YHDQTMLLVPLTLAANLPEGVIEVKADVAWLECKEACLPGDAAVSARLTVGPKAEPSK
                     EAAEIEKWKARIPVPNPAIEATVAWVLEPDGDSGEIAIEGRVSDGFVPTDFYPYADDA
                     YEIRPEVKPLEAPTGRFRFSKTLKRFATDFPTALPGLLIHPGTGHGDVRAHEVNLTFA
                     SAGPSASAADPDSSTAQTTQQSPRTLLWKMLVLGLLGGLILNIMPCVLPVIALKILGF
                     VQQSKEEPRRVRQLGLLYGLGVLTSFMVLAGVVIGVRSAGEAASWGMQMQNSFFRMAL
                     TVVVTLVALNLFGVFEVTLGGRAIGAAAGLASKEGNAGAFFNGVLATALATPCTAPFL
                     TVALGFAFTQPAHVIVLMFAATALGLAAPYMILSWHPAWLKFVPKPGRWMERFKVAMG
                     FPMLATAIWLFDLTAPAYGQAGVLWIGLFLVTLALAAWILGEFVQRGQRRRGLAGAIA
                     LCLLIAGYTVSLERQLRWRQPASQNATADVVRDSPEGLAWHRWSPEAVERARADGRIV
                     LVDFTAKWCLTCQANKRLAIDIPSVRVKLDELGGVAFRADYTNKDPRIAAELKRYERA
                     GVPLVLVFPRKVTGPAIILPTALTPGLVLEALGRAAGK"
     gene            complement(6924..8336)
                     /locus_tag="PKK20_03505"
     CDS             complement(6924..8336)
                     /locus_tag="PKK20_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNU98984.1"
                     /translation="MNAGNQSLASRRDFIKTTGQLAAASALAGVALPHVHAAEDNTIR
                     LALIGCGGRGCGAVANAMSAGGLVLGDSGGTERDASAGAAGPVRLVAMAELREDRLNQ
                     SHAALRQALGDRIDVPPERRFLGFDAYRHAIDCLRPGDVAMLTTHAAFRAPHLEYAVE
                     KGVNVFMEKDFAADPGGVQRILRAGEAAEKKNLKIAAGLMARHSSARQALIQKIRDGA
                     MGEVQLIRAYRMDPGYFMPPFSRNENELLWQLSPGHPYQFLWASGGIFIELMIHQIDE
                     CFWIKDSWPVSAHGVGGRFAGSADASQNLDSYSIEYTFADGTKALVTGRYIPRCHTDF
                     VTFVHGTQCAAKFSGDIHAPTCWLYKDQRTEPSNIAWRTPKETVNPWQAEWDVLLSAI
                     RNDRPHNETRRAALSNLGAIMGRAAVHTGQIVTWEQAIASKFQFCPNVDALTESSPAP
                     VQANPEGRYPAPVPGAWTEI"
     gene            complement(8561..10942)
                     /locus_tag="PKK20_03510"
     CDS             complement(8561..10942)
                     /locus_tag="PKK20_03510"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNU98985.1"
                     /translation="MKALNRLLWSALIFLFAHLQLAAAGNPIPNNSFEEHQGGRPRGW
                     RQQVWGGEGTGRVADAARTGSRSIMLSSDAGADISWSASVPVEPFSRYRLSGWIKTED
                     VRPMDGRGALFNVHDIQTVATKAVTGTTDWTRVEVEFDVEDHHVLQINCLLGGWGRAT
                     GKAWYDDLELAQLSRRDPPAPQITVDADQVREPLSPDIYSQFIEHLGRCIYGGIWAEM
                     LEDRKFYFPITADYRPYRSLRDSAFPVIGASPWRIVGAADGVSMNGEKPFVGRHTPQV
                     KAGTAIRQEDLGVTQGKRYTGYVWLRPHGGQRVSVDVTLAWGDDAAAKRTMAMALDAP
                     DYRRFEFAFTAGETTDKATFELLVKEGAALIGTVSLMPADNVEGMRADTLALLKDLKA
                     PIYRWPGGNFVSGYDWRDGIGDRDRRPPRTNPAWTGVEHNDFGMHEFIRFCRLIDTEP
                     WITVNTGFGDAYSAAAQLEYCNGAPDTYWGRRRAENGAPEPFGVKYWGIGNEMWGAWQ
                     LGHMALDHYVIKQNWVVDKMREVDPKIICIASGDIGPWSTGLLKSCPDHMDFIAEHFY
                     CQERPGLAAHVRQIPDNIRRKAEWHRKARQDLPELKDKDIRIAMTEWNYWYGPHVFGE
                     LGTRYFLKDALGIAAGVHEYARQSDIVGSAFYAQTVNVIGAIKTSRRNAAYETTGLVL
                     KLYRHHFGELPVATQTTGLVDAQAAWSRDRKTLTLGVVNPTLEPATVPLRIQGARLKG
                     RGTRWQIAGSDPMAFNDPDQAPRVAIEEQQALSVGEQIALAPCSITLFAFEAD"
     gene            complement(10945..11619)
                     /locus_tag="PKK20_03515"
     CDS             complement(10945..11619)
                     /locus_tag="PKK20_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98986.1"
                     /translation="MRQDKAGLWIATRHPATYRGAATVARWLVVVLAMVLLPLWTRGA
                     QPVLYSTDLFHPHDDPDDHFDLAVLYAIPGIDLKGIILDQGATQRHRPGRIPVSQLNR
                     LTGRRVPVAIGLPDKLRSPDDKALDQDADGQEGVRLILDTLEASPQPVALVTVGRVLC
                     FIGEASKSDFREHNVGLDPQAETRGNARFRVAIATRNLAQAALNSIMQCSYCLKKTPL
                     HPRPKN"
     gene            complement(11871..12374)
                     /locus_tag="PKK20_03520"
     CDS             complement(11871..12374)
                     /locus_tag="PKK20_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNU98987.1"
                     /translation="MILLIGYGNELRGDDAIGPLVARALAELRLPGTQVLALTQLTPE
                     LAELVSQASAAIFVDAALDGPPGQVEVVPCRPLDLRQPLGHLGNPESLLALCQAVFGA
                     SPSAWMVKVQVETTELGHPLSPAAQRGIPAAVDVITRLVGQLAPACPETASDDRSDHR
                     PTQDTEA"
BASE COUNT         2157 a         3820 c         4176 g         2359 t
ORIGIN      
        1 ccgaggatgt cggtcagggt tgtgacgaag atgctcgagg ccatggctgg atctatcctg
       61 cagcacctca aaccgaacgg gacgagcacg cctgagagga cgtcaacgcg ttggttgatg
      121 cccatcgccg cgccggccac gatttccaac aggaggcttc ctttccaagc ggagctgccg
      181 gctcggcttt tgatcttccc agatcggttg gcggcctggc atcgggcgga gtgatcgagc
      241 gccgtccgcg acctggaggt cagatcatct ctcgggagtg cgaagcctcg gctgttggat
      301 ggatcgactc ggccccaagg gctccaggtg cctgcccctt gaaatgcgcc cctggctttg
      361 ccagactcgc caccatgccg aaggaaatcg ggccatcatc gtacgagtgc gattgtgggt
      421 acgtcgcaca gttctccgag gccaccatcc tggcgttgaa gcaacgcagc ctcatgaagc
      481 gaccgtggct ctcggatggc ggggaagccg gggggcacat cgtggtgttc gaggggggca
      541 agatgatgac gatgctctgc ccaaaggaaa ggtacaatga aaagcctgcg ccgtgcttca
      601 cccgactgca gggccggtac ttggcgttta tccaccaata caccacgcta catcgggtgc
      661 ctccggcgga gcacgagatg cagcggttct tccaggtctc gccgcccacc gtgcaccaga
      721 tgatcctgac cctggaaagg aaagggctga tcaagcgcac gcctggcaag gcccgatcga
      781 tcaaggtgtt ggtcgcagca gagcaattgg agaggctgac gtgacccgaa ccaccatcgc
      841 tttcgacttc catgtcgccc tcgtcggcgc acgcgagatc tggcgatgca tccggaaccg
      901 aacctgcctg tcctggagaa gcgcggcgaa tcacccaatg agttcgagca gtggccgcca
      961 gggaggcttt gagaaacctc gcgcggttct gatcgcctga gttcgatcct gagtgtcgag
     1021 cgggactcct tgaccctgat ctcctatgaa caacgacaag ctcgagtacg ctgatctcct
     1081 cgagttgctg atcaacggcc ctgttggcgg gccccgacgt tgggctgcct ggctggtcct
     1141 gcggatgttg gagacgaagg gcaactgttg ctatcggagc gacgaccacc tccgcggcac
     1201 ggagcgtgcg caagcggcga ttgcctctct gcgagccgcc gcacgcgcgc tctccgtcgc
     1261 cgaacaagga ctgctgttgc gggcgatact gacgctgtca tgggaacaac tgcgtcggaa
     1321 caccggcttc agcctccgag tgcccgggat cctcgttgaa ctcggcaagg acctcggtgt
     1381 cctgccttgt ctggctgccg ccgaatccgt agtcgaccaa ggcaacgacg ctgtgcgccg
     1441 gtatccgctg acgggtctgc tgccggaggt gcggcagccc ggcagtggcc tcatctttag
     1501 cctgagtgcg cgggaacgca aacagctcgg agtttccgcg gacacgatcg aatttgaccg
     1561 actgaccgtc gcggtcaaga gcttccgtgg ctgcggatgg gaggcgaacc tgcgcccttt
     1621 ggttgcctgg tggatcgaga gccttgatca cagagacgcg gctcgcgtgg tcagaggatt
     1681 gttcccggac atacgcaggg cggccaagga tgaccaggcg cgatggcgag cactcggact
     1741 gctggattgg ctggtggacc atccggatca cccgacagcc ggcgaggcaa tgaccgtcgc
     1801 ccgggaggtc gctcgggccg atgtccgcaa agccgcagcc gatctggcgg cggcgttggg
     1861 gcagtgggaa gtgctcgaat ggctcaccgg gagtgatcgc gaccgaagcg tgcgggagcg
     1921 ggccgagaag ttgctccagg gcagggtcgg tccaggagca tccggcggcc aggacgaact
     1981 ctttggatag ggcgtaagac gaaattcttc gttcaagctg cgagggggat ggcgtcattg
     2041 agggaaacgt gcctgttgag cctccatttc cagaaggcgt cgaggcgccg actcgcgtgg
     2101 atgcagcgga aggcgaggat gttctcggct ccggtgacac cccagtacat gccagactgc
     2161 ttgcagcgtg agccgatcgc cgttttgcag cccgcctccg ccactccaga gccaatgaat
     2221 ggatgaaatt gcggggcgcg tccggcagag ggaccagcac gcgaaagctg tctgccccaa
     2281 tacgcctcac gcatgcgggg cgtgacttcg ttggccgagg gatggcagga tggctgccgg
     2341 caggcgctgc ctgccaccaa tgcgcgtcct gcttgtcaac accaaccgaa tgaagcccgc
     2401 aatcgcgccg ctcggtttgg attacctggc cgacgctctc gaggcggctg ggcacgaacc
     2461 gaggctggtc gacctctgtt tctcgcagga catcgccgcc gatctcgagg cggccgtgcg
     2521 tcttggcgcg cccgaagtca tcggggtcac cgtgcgtaac accgatgact gctacatgtc
     2581 cggccaggcg ttcttcctgc ccgagatcaa gcgggcgatc caaatcctcc ggggcagcac
     2641 gacagcccca atcatcctcg gcggcgttgg tttctcggta gcgcccatgg ccgtcatgga
     2701 gttctgcggg gccgacttcg gcatggccgg cgacgccgag tttgcgctgg tcgagttcct
     2761 gcgtgcgatc gagcaacgtc gcgactggtc cgccgtgccc ggcctggtct atcgcgatcg
     2821 gggccgctgg cggctgaacc cggtcgtccc cggcgacatg gcccgattgc ccgaacgccg
     2881 ccgggctttg gtggacaacg cgcgctattt ccgcgagggc gggcaggccg ggttcgagac
     2941 caagcgcggc tgtgggatga ggtgcctgta ttgcgccgat ccggtcgcga aagggcggac
     3001 gacgcgttgc gcgccgccgg gcctggtggt tggtgagttg tcggcgttgc tggcccagaa
     3061 cgtcgatcat ttccacacgt gcgacagcga gttcaacctg ccgctcgaac acgcgcgcgc
     3121 cgtgtgccag gcgatcgtgg acgcgggttt gggcgaacgc atccgctggt atgcctactg
     3181 cgcgccgacg cccttcagcg acgatttggc gacgctatgc cggcgggctg gatgtgtcgg
     3241 catcaacttc ggggtcgaca gcggttgcga ctcgatgctg cgacggttgg gtcgccactt
     3301 caccacggcc gatctgctcg ataccgccgc gacctgccgc cgccacggtc tggcgtccat
     3361 gtacgatctg ctgctgggcg gtccaggcga aacgcgggcc acggtgcgcc agaccatcga
     3421 cctcgttcgc cgggcggcgc cggactgcgt tggactctcg ttgggggtgc gggtgtacga
     3481 aggcacaccg ctggcggagt gggtgcgctc tcaggcggag gtcccatccc acccggactt
     3541 gtacggcgcc aaggaagccc acccgcaatt gttggcaccg gtgttctatg tcgcgccggg
     3601 attggcggag gatctcgtcg gctgggtcca cgaactggtc gcgggcgaca gtcgtttctt
     3661 tctgccaacg aacactgcaa cccaccagaa ctacaactac aacgccaacg agacgttggt
     3721 gcgggcgatc gcgcaagggg cgcgtggcgc ttactgggcg atcctgcgcc gtctacgaac
     3781 ggcagcgggg tgagatccgc ggatctggtg tgtcaaaaac acttcgccga cgggaccatc
     3841 cggtcccatc ggcgaagacc aacccatgta acctaaccaa ccagacaacc agtccactgg
     3901 ttgtatctca gaaaatagcg taattcgtgc caactttcgg cagtgcacac ccttggctct
     3961 cctaaggtat accgccgagt attcatgcgc ctttcagatt ctaggatctt gaagatgaac
     4021 gtgttaatca gcgtatttga cgctgcatcc gtagggcttc gtctcggctg tcggcaccgg
     4081 tttgccggcc atgagggcct cgattgcctg gcgcacatag ttccgcgctg tgcgagggtc
     4141 tccagaactc gctgggcgat cgtcgatggc gccctgatag gcgaccaagc cttgctggtt
     4201 gatgacgacc agatgcgggg tcgtcttgag cccatacttc ctgccgatag cgccgctgct
     4261 gtcatcgatc caggctgtcg ccttgatgcc tgttgccgcg aattccttgc gggcggcgtc
     4321 cggttttctg taggacggat ttcccggatg cgttgagttg acgaccaacc agacgacacc
     4381 tttgctgacg acgctcgatt ggagggcttg catggcgccg gatttgtagt ggttggcgca
     4441 gaatgggcag tcaagattgt aggcctcgat cacgaccaca ttgccacggt agtcgctaag
     4501 cctatggact ttgccctgga tgtcggtccc tgaaaaatcc ggcgcgggct gcccgatctt
     4561 cacgtcggca aggacgatcg attgagccac cataaagccg gtcaacatgg gaatcaatct
     4621 cttcatatgg ttcgcattcg ttttctgacc gaggcctgtc tgtttctgcc cggtcgtatc
     4681 gcactcagac agagtagaag cttgaagagt tcaaattatt cattgaattt atcaatcgag
     4741 tcatttaccg gcagcccggc ccagggcttc gaggacaaga cccggcgtca gggccgtcgg
     4801 cagaatgatc gccggtccgg tcactttcct gggaaagacc aggaccaacg gtactcctgc
     4861 ccgttcgtag cgtttgagtt ccgcggcgat gcgggggtct ttattggtgt agtcggcccg
     4921 aaaggcaacc cctcccagtt catcgagctt gacgcgaacg gacgggatgt cgatggctag
     4981 tcgtttgttg gcttggcagg taaggcacca tttggcggtg aaatccacca ggacgattct
     5041 gccgtcggcg cgggcgcgct cgacagcctc gggcgaccag cgatgccagg cgaggccctc
     5101 ggggctgtct cgaaccacat ccgcggtggc gttctgactt gcgggttggc gccatcgcaa
     5161 ctgccgttcg aggctcacgg tgtagcctgc gatcaggagg cacagcgcga ttgcgccggc
     5221 cagaccgcgg cggcgttgtc cgcgctggac gaactcgccc aagatccagg cggcgagggc
     5281 gagcgtgaca agaaacaaac ctatccagag caccccggcc tggccgtagg ccggcgcggt
     5341 aaggtcgaag agccagatcg cggtggccaa cattgggaat cccattgcca ccttgaaccg
     5401 ctccatccaa cggccgggtt taggtacgaa tttcagccag gccggatgcc aactgaggat
     5461 catgtaaggc gcggccaggc ccaatgcggt ggcggcgaac atcaacacga tcacatgggc
     5521 cggttgtgta aacgcgaacc ccagcgccac cgtcaggaag ggggccgtgc atggggtcgc
     5581 cagcgcggtc gccaacaccc cgttgaaaaa ggcgccggcg ttgccttctt tcgaggcgag
     5641 tccggcggcc gcaccgatcg ccctgcctcc cagagtgacc tcgaaaacgc caaacaaatt
     5701 gagcgccacc agggtgacca cgaccgtcag cgccatgcgg aagaaggaat tctgcatctg
     5761 cattccccaa ctggctgctt ccccagcgct tcgaacgccg atcaccacgc ccgccaagac
     5821 catgaacgac gtcagcacgc ccaacccgta gagcagaccc agttgccgga cgcggcgcgg
     5881 ttcctctttg ctctgctgga cgaagccgag gattttcagg gcgatcaccg gcagcacaca
     5941 cggcatgatg ttgaggatca ggcccccgag caggcccaaa accagcatct tccacagcag
     6001 ggtccggggc gattgttgcg tggtttgggc tgtcgaactg tccggatcgg ccgcgcttgc
     6061 gctagggccc gcggatgcga aggtcaggtt gacttcgtgg gcacgcacgt ctccgtggcc
     6121 cgtccccggg tgaatcagca ggccaggcag cgctgtcgga aagtccgtgg cgaaccgttt
     6181 gagggttttc gagaagcgaa aacgaccggt cggcgcttcg agcggcttga cctcgggcct
     6241 gatttcgtac gcatcgtcgg cgtagggata gaagtcggtg gggacaaaac cgtcggagac
     6301 ccgaccctcg atagcgatct cgccggagtc accgtcaggt tcgagaaccc atgccacggt
     6361 agcttcgatg gcgggatttg ggacgggaat ccgggccttc cacttctcga tttccgcggc
     6421 ttccttcgag ggttcggcct tgggcccgac ggtgagacgg gcgctcaccg cggcgtcgcc
     6481 gggcaggcac gcctccttgc attcgagcca cgccacgtcc gccttgacct cgatcactcc
     6541 ttcggggagg ttggcggcga gcgtcaacgg cacaaggagc atggtctggt cgtgataaat
     6601 gaaggtcgtc atcccggccg cggtgtgcga ctcggggagc ggccagtgga ttgcgcccgc
     6661 gctcaccccg ggtggcaggt gccactcgat gctggttgcc agacccgact cgccgggatt
     6721 gcgccagtag gtgtgccagt ccggcgccat ggtgaggtgg atgccggccc agaccacctc
     6781 gccggcgcgc gccgtgtcgg ccgacaggac caggcgcgcg ctggtattgg gctgggcggc
     6841 ggtggcggcg gtggccagga gccagagcgc ggcggccagt gcgacggtgc tccgtgatgt
     6901 catgcgattc aagcgcgagg atttcagatc tccgtccagg cgcccgggac cggggcggga
     6961 tagcggcctt cggggttcgc ctggacgggc gcggggctcg actcggtgag agcgtccaca
     7021 ttggggcaga actggaactt ggacgcgatg gcctgttccc acgtcacgat ctggccggtg
     7081 tgcacggcgg cgcgacccat gattgcgccg aggttcgaga gggcggcgcg tcgggtttcg
     7141 ttgtgggggc gatcgtttcg gatggcgctc aggagcacgt cccactcggc ctgccagggg
     7201 ttgacagtct ccttgggtgt tctccaggcg atgtttgacg gctcggtgcg ctgatccttg
     7261 tagagccagc aggtcggcgc gtgaatgtcg cccgagaact tcgccgcgca ttgggtcccg
     7321 tggacgaacg tgacaaaatc ggtgtggcat ctcggaatgt agcgcccggt gaccagggcc
     7381 ttggtgccgt cagcaaaagt gtactcgatc gagtagctgt caagattctg gctggcgtcg
     7441 gcgctgccgg caaaccgccc gcccacccca tgcgccgaca ccggccagga gtccttgatc
     7501 cagaaacact cgtcgatttg gtggatcatc agttcgatga agatgccacc ggaggcccac
     7561 aggaactggt aggggtgccc ggggctgagc tgccagagca gttcgttttc attgcgcgag
     7621 aagggcggca tgaaatagcc ggggtccatg cggtaggccc ggatgagctg gacctcgccc
     7681 atggcgccat cccgaatctt ttggatcagc gcctgccgcg ccgaggaatg acgggccatc
     7741 aagcccgcgg cgatcttgag gttctttttc tcggccgcct ccccggccct gaggatgcgc
     7801 tggacgcccc ccgggtccgc cgcgaagtcc ttttccatga aaacgttcac gcccttctcg
     7861 accgcgtatt cgagatgcgg agcgcggaag gccgcatggg tggtcagcat ggccacgtcg
     7921 cccggacgca agcaatcgat ggcgtgacgg taggcatcga aaccaaggaa gcggcgttcc
     7981 ggcgggacgt cgatccgatc gcccagggcc tggcgtaacg cggcgtgcga ttgattgagc
     8041 cgatcctcgc gcagttccgc catcgcaacg agccggaccg gaccggcggc gcccgccgat
     8101 gcgtcgcgct ccgtgccgcc gctgtctccg agcaccagtc cgcccgcgga catcgcgttg
     8161 gccacggcgc cgcacccgcg ccccccacac ccgatcaagg cgaggcggat ggtgttgtct
     8221 tcggccgcgt gcacgtgcgg gagggcgact ccggccagcg ctgaggcggc tgcaagttgc
     8281 cctgtggttt tgatgaaatc ccggcgggaa gcgagtgact gatttccagc attcatggag
     8341 gccatgctcc atcggatgct gaaatgctgt caatccgcgt ttctcctccc ggaaccccca
     8401 tagccgccag cattcaatcg gaatccactg ccgatggccg cggcggttgt gctcgaggcg
     8461 cagcgccggg tgctgctcat tcgatggaag catccgccgc gccgcagcct ctggtgcctg
     8521 cccatgggct tcgcggagtt gggggagaca atcatgcggg ctagtcggcc tcgaatgcga
     8581 acagcgtaat gctgcaaggc gcgagtgcga tctgttcgcc caccgatagc gcctgctgct
     8641 cctcgatggc gacgcgcggc gcctggtcgg ggtcgttgaa tgccattggg tctgacccgg
     8701 cgatctgcca gcgcgtgccg cgacctttga gccgggcgcc ctggatgcgg agcgggacgg
     8761 ttgccggttc gagggtggga ttgaccacgc cgagcgtgag cgtcttgcgg tcacggctcc
     8821 aggccgcctg ggcatcgacc aggccggtcg tttgcgtggc cacgggcagt tcgccgaagt
     8881 ggtgccggta gagtttgagc accaggcctg tcgtttcata cgcggcgttg cggcggcttg
     8941 tcttgatcgc gccgatgacg ttgaccgtct gcgcgtaaaa cgcggagccc acgatgtcgc
     9001 tctgccgggc gtactcgtgc acgcctgccg cgatgccgag cgcgtccttg agaaagtagc
     9061 gcgtgcccaa ctcgccgaag acgtggggcc cgtaccagta gttccattcg gtcatggcaa
     9121 tgcggatgtc cttgtctttg agttcgggca ggtcctgccg tgccttgcgg tgccattcgg
     9181 ctttgcgccg gatgttgtcc ggaatctggc gcacgtgggc ggcgaggccg gggcgctcct
     9241 gacaatagaa atgctcggca atgaaatcca tgtgatccgg gcatgatttg aggaggccgg
     9301 tgctccacgg gccgatgtcg cctgaggcga tgcagatgat tttgggatcg acctcgcgca
     9361 tcttgtcgac gacccagttc tgcttgatca cgtagtggtc gagggccatg tgtcccaact
     9421 gccaggcccc ccacatttcg tttccgatgc cccagtattt cactccgaac ggttcaggcg
     9481 ctccgttttc ggcgcggcgg cgtccccagt aagtgtcggg agccccgttg cagtactcga
     9541 gttgggcggc tgccgagtag gcgtcgccga acccggtgtt gacggtgatc cagggctccg
     9601 tgtcgatcaa ccggcagaac cggatgaatt cgtgcatgcc gaagtcattg tgctcgaccc
     9661 ccgtccaggc cgggttggtc cggggcggac gccgatcgcg atcgccgatt ccgtcgcgcc
     9721 agtcgtagcc gctgacgaag ttgccgccgg gccagcggta gatcggggct ttcaaatcct
     9781 tcaacagggc gagtgtgtcg gcgcgcatcc cttcgacgtt gtcggcgggc atgagggaga
     9841 ccgtgccgat cagcgccgcg ccttccttga ccagcagttc gaatgtcgcc ttgtcggttg
     9901 tctcgccggc cgtgaaggcg aattcgaagc ggcggtaatc cggggcatcg agggccatgg
     9961 ccattgttcg tttggctgca gcgtcatccc cccacgcgag cgtcacatcg accgacacac
    10021 gctggccgcc gtgcggcctg agccagacgt aacccgtgta gcgcttgccc tgcgtcaccc
    10081 caaggtcttc ctgccggatg gcggtgcccg ccttgacttg gggcgtgtgc cgtccgacaa
    10141 acggcttttc tccattcatg gagacgccgt cggccgcgcc aacgatgcgc caaggcgagg
    10201 ctccgatcac cgggaaggcc gaatcgcgca acgagcggta cgggcgataa tcggcggtga
    10261 tggggaaata gaacttccgg tcttcgagca tctcggccca gattcctccg tagatgcagc
    10321 gtccgaggtg ttcgatgaac tggctgtaga tgtcgggaga aagcggttcc cggacctggt
    10381 cggcgtcgac ggtgatctgg ggagcggggg gatcccggcg gctcaattgg gccaattcga
    10441 gatcgtcata ccaggccttg cctgtggccc ggccccagcc gcccagcaga cagttgatct
    10501 ggaggacgtg gtggtcttcg acgtcgaact cgacctcgac acgcgtccaa tccgtcgttc
    10561 ccgtcacggc ctttgtggcc acggtctgga tgtcatgcac attgaacagt gcgcctcgcc
    10621 catccattgg ccgcacatcc tccgtcttga tccagcccga gaggcgatag cggctgaacg
    10681 gctcgaccgg gaccgaggcg ctccacgaga tgtcggcccc cgcgtccgac gacaacatga
    10741 tgctgcggct gccggtccgg gctgcgtccg ccacccgccc ggttccttcg ccaccccaga
    10801 cctgctgccg ccagcccctc ggacgtccgc cctgatgctc ctcgaaggag ttgttcggga
    10861 tcggatttcc cgcagccgcg agctgtaaat gcgcgaagag aaagatcaga gcggaccaga
    10921 gaagacggtt cagtgctttc atattcagtt cttcggcctt gggtgaagcg gtgttttctt
    10981 tagacaatag ctgcactgca tgattgaatt caaggctgcc tgtgccagat ttcgtgtcgc
    11041 gatcgcgaca cgaaatctgg cgtttcctct ggtctcggcc tggggatcga gtccgacatt
    11101 gtgctcccga aaatcggact ttgacgcttc gccaatgaag cacaagaccc tgccgaccgt
    11161 tacgagcgcg accggttggg gcgacgcttc gagggtgtcg agaatcagcc gcacgccctc
    11221 ctgtccatcg gcgtcctgat ccagggcctt gtcgtcgggc gatctcagct tgtccggcaa
    11281 accgattgcc accggaacgc gccgcccggt gagccggttg agctgagaca cggggattcg
    11341 cccgggccgg tgccgttggg tggcgccttg atcgaggatg atgcccttga ggtcaatgcc
    11401 ggggatcgca tagagcacgg ccaggtcgaa atgatcgtcc gggtcgtcgt gcggatggaa
    11461 gagatcagtc gagtagagga cgggttgggc gccacgagtc cagagaggca gcaagaccat
    11521 cgccaggacg acaacaagcc accgcgccac cgtcgcggcg ccgcggtaag tggcggggtg
    11581 gcgtgtcgcg atccacagcc ccgccttgtc ctgtcgcatt cgccggatcg aatcggtgga
    11641 ggcggatcgg gatcgtgtcg gtcgcaggtt acactgcaag aagactcccc ctgggcagtc
    11701 tttgtcgctg ccttcgtcgc aacctttgtc gcaagggggc gtggcggtcg acaagggtgc
    11761 cgacggaggt ggcgacaacg gtgttgtcgt tgcgaacccc attctcattt gatgtggttt
    11821 gccaaaatgg catgtcaggt tcttagggct ctgtttgcac ttgatccgcc tcacgcttcc
    11881 gtgtcttgcg tggggcggtg atcgctccta tcgtccgacg cggtctctgg acaggccggg
    11941 gcaagttgtc cgacgagccg ggtgatgaca tccacggcgg cggggatgcc gcgctgcgcg
    12001 gcgggggaga gggggtgtcc gagttcggtg gtctcgactt gcaccttgac catccaagcc
    12061 gatgggcttg cgccaaacac cgcctggcaa agggcgagca gtgactcggg atttcccaga
    12121 tgcccaaggg gctgtcgcaa atcaaggggc ctgcaaggga cgacctcgac ttggccaggc
    12181 gggccgtcga gggcggcatc gacgaagatc gcggcggagg cttgcgacac gagttccgcc
    12241 agttcggggg tcagttgcgt gagggcgagg acttgtgtgc ccggcaggcg caactcggcc
    12301 agagcccggg cgaccagggg gccgatggcg tcgtcgccgc gcaactcatt gccatagccg
    12361 atcaggagta tcatggctgc gatggtgggt ccgatgcggg cgcgatggcg gttcgaggtt
    12421 tcgccgtgag gctctcgata tggcgcacga ggtcgggcag cgcatcgtag cggtccaagc
    12481 gcacgcctgc cgcgcctccg cccggatgcg cc
//
LOCUS       DAOQRO010000057        12470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4181_length_12470_cov_4.380588, whole genome shotgun sequence.
ACCESSION   DAOQRO010000057 DAOQRO010000000
VERSION     DAOQRO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_12470_cov_4.380588"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKK20_03525"
     CDS             complement(<1..592)
                     /locus_tag="PKK20_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNU98988.1"
                     /translation="MNATFPALTVVCFGLVLSAASLAAEASWPKEVPASSPRKSKKIE
                     PASKVYPSVGAIERHDVSIDTLISPDAKVERLAGGFEWAEGPVWNRKTRTLLFSDVPR
                     NVIFEWKESVGTRDYLYRSGYTGARNEGKEPGSNGLTFDADGRLVICMHGDRVVARLE
                     RDGKLTILADYYLNRRFNSPNDLVYNSKGNLYFTDPP"
     gene            complement(956..2983)
                     /locus_tag="PKK20_03530"
     CDS             complement(956..2983)
                     /locus_tag="PKK20_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNU98989.1"
                     /translation="MPKSPIQRRTFLARVSLGTATLAAAPLSRVGIAAPSSAKGAAAS
                     GLAEPAFRPLPLGSIEPAGWLRRQLQLQANGLSGHLDEFWPDVAQSQWFGGKAEGWER
                     APYWLDGLIPLAWTLGDEGLQAKARRRVKQILDGQRPDGWYAPYPVDASAKAYDLWAI
                     LLVNKVLVQYHEATGDDAVLRAVVSNLKAVHGVLDRSPLFNWGRFRWFEGLIAAYYVY
                     EQTGETWLLDLARKLRAQGFDYKSFFEGEDVLSPTPRRGLWTWEKHVVNTGMALKAHT
                     LAWRLSRDEDDRGHTQRMLRILDRCHGQVNGMFSGDECLAGRSPTQGTELCAVVEALY
                     SLELALALTGDPALGDRLERIAFNALPATFTPDMWAHQYVQQANQVQCMINPEHMWST
                     NGPESNIYGLEPNYGCCLANMHQGWPKFAAHLWMRMPDGGLAAVAYAPSTARFTVRGQ
                     PVRVTLEGDYPFRETVRLVVSTEQPARFPLALRVPAWAENPTVRVGAGPAKRLKAGSF
                     HRLNQEWKGSVAIELRFPMPVRATRRYNQAVALERGPLVYALKLGETWTRVNADKPHR
                     DLPHGDFEVRPSTPWNYGLVVDEGRPETSVAFEERPVGDKPFSPEGAGVIAKVKGRRL
                     PGWQLERGWAQEVRPELQSSKEPIEELTLVPYGCTNIRVTEFPRLQVPGTM"
     gene            3491..4450
                     /locus_tag="PKK20_03535"
     CDS             3491..4450
                     /locus_tag="PKK20_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU98990.1"
                     /translation="MLEIENLRIEFETGAQPVPVLDGVSLDIGSGETVCLVGESGSGK
                     TVTALAIARLLPCPPARYAGGRILVNRRDALRMSARELRTIRGRVVSYVFQDPAVALN
                     PVLRAGAQVLETLALHRPEAAHTDEVISLLRWVGIPAPELRIRDYPHQLSGGMQQRLM
                     LAMALASHPRLLVADEPTTALDVTVQAQILELLRALRRQLNMSILLITHNLGLVGEIA
                     DRVVVLYAGQIIEEAPARELLSHPRHPYTRALMKSVPELGRDVPRLEAIPGSVPRLGD
                     WPAGCRFHPRCPVARPGCSREAPALASVAPHQRVRCPFWDHAP"
     gene            4543..5448
                     /locus_tag="PKK20_03540"
     CDS             4543..5448
                     /locus_tag="PKK20_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU98991.1"
                     /translation="MSFELAAGETVGLVGESGCGKTTLGRAVVRLIEPTAGAIEFGGI
                     DLASLRPGQLRPVRRRLQMIFQDPYGSLNPCLTVEQIIGEALDIHGLARTREALRARV
                     GFLLDHVGLAPADARRYPREFSGGQRQRIGIARALAAEPQLIVCDEPVSALDVSVQAQ
                     IVNLLHDLQREHGLAYLFIAHDLALIEHLSRRILVMYLGRVVEAGDTKMICRTPAHPY
                     TQALISAVPRLDPVANRRRIVLTGEVPSPITPPSGCGFHPRCPIAEPRCGREVPALRE
                     IASGRWAACHLAWGGNRMATTGGVV"
     gene            5460..10286
                     /locus_tag="PKK20_03545"
     CDS             5460..10286
                     /locus_tag="PKK20_03545"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNU98992.1"
                     /translation="MISRFRESDAKGRWGEGSRLTVASAAVLWLALGIAGSGRADVWI
                     NEFVADNENGLRTQAGVAADWIELANDSSQPVDLSGWHLTDRADAPTKWRIPDGTIIP
                     ANGYLVVFADSSAHSITNNELHANFSLSANGEYLGLIRPDGVTVAHEFAPAFPPQLRD
                     VSYGRGTPREREWIGPSTPARYRIPNAGGTAPWTDAVGALGFSETKGAFTVRYYEMRN
                     DIQNVDAAEAMVADSRSWKTDAAYPIVGQYDTIDFHANSASGFFTENVLFPNHSFAGQ
                     DKDCFVLVAEGAIHVPAPGLWTFAVGSDDGFRLRISGHGVDFVSEYLTGRSFDTTLAT
                     FNFPVAGVYDLRLIYYENHGGASVEFSVAQDFQASLVPGLFHLTGDPAGGILHAGAIG
                     SFVQTDIGPAMRGVNARVDAEWLFTMDESPGLDDRFTLSVRSADGFVASLNGVTIGAL
                     NAPDNLVWNSTAASARPLEEAVQWVTYDVPVSSVSAGTNILAISGLNDSVDDPNFLIA
                     PRIASRSAESIAAYFKEPTPGAANGRAYAPPTPVVTASEPRGYKTSPFTVSLVCSNTT
                     AEIRYSLDGSVPDTNSLLYAGPISVSKTTTLRAAVVDSDSVRQNVTTVTWLFLEDILE
                     QGSTPPPGWPASRQVNNHVMEYGLRREIVTRDSARLRNGMTNAIASISLVTDLDNLFS
                     SQRGIYVNPGNDGIAWERPVSIELIDPVRGGESEFQIDGGLRIRGAYSRSSGNPKHSF
                     RLFFRSAYGQGKLRFPLFDDEGASEFDKVDLRTSQNYSWAYENSNRETFVRETFSRDS
                     QRDMGMPYTRSRYYHLYINGQYWGLYQTQERGDADYAETYLGGDAEDWDCIKTSQPGY
                     TTTASDGTFDAFYALHNIAINQGFTGAYASNYQRVKGLNPDGTPNPSYPVYLDEDNLI
                     VYMLTALYTGDPDSPVSIWGNFPNNLYGLFNRLHPDGFKWLRHDAEHSLGAHGSYGVT
                     ADTTTAGASFTTRGNFNPATLHQRLCQHPRYRDRFADLVQKHLYGDGALTPKNAQQRF
                     RNRMDEIDLAIIAESARWGRGKTRDATWLPACNEVLNTYLAQRRDIVIAQFRNRGWFP
                     SIDAPGYSVMNSEVPAGEVVRLLCNTTFYYTIDGSDPRLEDGSINPSAIEVKSSTGPT
                     GPRQVVRRGAEWRYFDLGTEPAPAAQLDWRDPDFPDADWARGPAILGVPGTSPANTVA
                     TRTRRYVNGVAAPQVTTTYFRHSFILDSTNDAPNLLIDILRDDGAIVYLNGVEILREA
                     MPPGPVTYATYSSAVVGPPDQNTYFSRSVNFAHLLRPGANVVAAEVHQCNAGSSDLYF
                     DFALTIPGQQAHVFAEIAVSKDMTIRARALDGTEWSALSENVLRVERPPVNYTALRVS
                     ELMYAPPAPDDESPYVSDDFAWIELCNAGATPLDLEGVRFDDGISHTFAPCVLEPGAR
                     LVLAANPDAFATRHPTNSIHLVAWTGGNLARGGETLELVDPDGHTILTFAYSRFWYPE
                     TFNTGRSLVAVDLAAAEPLWSTPDNWRPSSALLGTPGLPEPPVFIEARVADTRLHASA
                     IGIDRTSELWFSEDLETWSLCDPAAWSQTNGLFTVDLEHPSLIDRTRCFFQIRVPQ"
     gene            complement(10287..10868)
                     /locus_tag="PKK20_03550"
     CDS             complement(10287..10868)
                     /locus_tag="PKK20_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98993.1"
                     /translation="MKTPSVVPRFVLAALVLTVVCWWAPGTTFAQPASGTGGGAPGAG
                     WRGFDPSQIQNFLLQSYRDRLEINDDAEWDVIRERIQKVIEARRDTAFSGMGMLAGMF
                     RRGGRGEGAEGQAGPGANRGLAGLLPAPSAEEEALQKAVDAKASSAELKVALAKYQEA
                     RRRQQSQLEKAQADLRAVLSVRQEAIASLSGLL"
     gene            complement(11092..11385)
                     /locus_tag="PKK20_03555"
     CDS             complement(11092..11385)
                     /locus_tag="PKK20_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98994.1"
                     /translation="MCKKPGPLRFASGRTDNSWLAEEAWIVFIPKSSDLCLPARATLA
                     AGKVSVIQVRGLSDSASGRRVVAVPSQAANPFAAHNAGPSTALRLEVEFHPAS"
     gene            complement(11432..12214)
                     /locus_tag="PKK20_03560"
     CDS             complement(11432..12214)
                     /locus_tag="PKK20_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU98995.1"
                     /translation="MRSVSSLPNSARALEARRGRAFTLIELLVVIAIIAILASMLLPA
                     LSKAKSKAIQIKCTSNMRQLGIAILMYANDYRDQFPDCAGAYWPWDLPVPAANAFVRN
                     GGNRKILYCPGFSQQDNDELWSFSTGVTNELADDGVGGYRVIGYAVAFKGSGRLLATN
                     ITESLNPAPWRMSDGSLYNPGASERVIAADGTLSSGSNQADRSRNNYTSVDGGWTKHH
                     RAAHLNGRIPAGGNILCLDGHAEWRKFPRMVIRTYDTPSFWW"
BASE COUNT         2211 a         4023 c         3929 g         2307 t
ORIGIN      
        1 aaggcggatc ggtgaagtaa agatttccct tcgagttgta gaccaggtca ttcgggctgt
       61 tgaatcgccg gttgaggtaa taatcggcca ggatcgtcag tttgccgtcg cgttcgagtc
      121 gcgccaccac gcgatccccg tgcatgcaga tgaccaaccg cccatcggca tcgaaggtca
      181 atccgttcga gccgggttcc ttgccttcgt tgcgggcgcc tgtgtaaccg ctccggtaaa
      241 ggtaatcccg cgtgccgact gactctttcc actcgaaaat cacattgcgc ggcacatcgg
      301 agaacaacag ggtcctggtc tttcgattcc agacgggccc ctccgcccat tcgaatccgc
      361 ccgccagccg ctcgaccttg gcgtccggtg aaatcagggt gtcgatcgac acgtcgtgcc
      421 gctcgatggc gcccacgctg ggatacactt tgcttgccgg ctcgatcttc ttgctcttcc
      481 tcggcgagga agcggggact tccttgggcc aggacgcctc tgccgccaac gaggctgccg
      541 atagcacgag accgaagcac acgacagtca atgcagggaa cgttgcgttc atattgaaat
      601 cgcgcacgga ggatcagacc cagcgcatcg agggtcaagg caaaagccgg gcaaaagcct
      661 gcatcccacc gccaattcat gaaggcggaa aggctggagg ggggcgactc gtcccccgac
      721 gacgggcgtg aaggcgatgt ctgtcggcga tgaggccctg cctgccctgc ctccatccca
      781 gatgccatga tcgatcccag gccccaaggg cgatgtcacc acccgacact gacctgcaaa
      841 ttccgcatct tcgccaactc acccaacgca cccaatcccc gcggcggacg cgccatatcc
      901 cgccgccgcg gcccaaatcc gaccccaaac tcccggaacg cctcgttcac atggttcaca
      961 tggttcctgg aacctggagc ctgggaaact cggtcacccg gatattcgtg caaccgtaag
     1021 gaacaagagt gagttcttcg atcggttcct tcgaggattg gagttcaggg cgaacttcct
     1081 gggcccatcc gcgttcgagc tgccagccgg ggagccgccg gcctttgact tttgcgatca
     1141 ccccggcccc ctcgggagag aacggcttgt ccccaacggg ccgttcctcg aaggcgacac
     1201 tggtctccgg acggccttcg tccaccacga gaccgtaatt ccacggtgtc gaaggacgga
     1261 cctcgaagtc gccgtgcggg agatcgcgat ggggcttgtc cgcgttgacg cgggtccagg
     1321 tttcacccag tttcaatgcg tagacgaggg ggccgcgttc gagggcgacg gcctggttgt
     1381 atcttcgggt cgctctgacg ggcatcggga accggagctc gatcgccact gatcctttcc
     1441 actcctgatt caggcgatgg aaggagccgg ccttgaggcg tttggcaggg ccggcgccca
     1501 cgcgcaccgt ggggttttcg gcccaggcgg gcacgcgcag ggccagaggg aagcgggcgg
     1561 gctgttcggt ggaaacgacg agccgcactg tttcgcggaa gggatagtcg ccttcgagcg
     1621 tcacccgaac gggctgacct cgcacagtga atcgcgccgt gctcggggca tacgccactg
     1681 cagccaagcc gccgtccggc atgcgcatcc agagatgggc cgcaaacttg ggccagccct
     1741 gatgcatgtt cgccaggcaa cagccgtagt ttggttcgag tccgtagatg ttggactccg
     1801 gaccattggt ggaccacatg tgctcggggt tgatcatgca ctggacctgg ttggcctgtt
     1861 gcacgtactg gtgagcccac atgtccggcg tgaacgtggc cgggagggcg ttgaatgcaa
     1921 tacgttccaa ccggtcaccc aacgccgggt cgcctgtcag cgccagggca agttcgaggg
     1981 aatacaacgc ttcgaccacc gcgcacagtt cggtcccctg cgtggggctg cgtccggcca
     2041 ggcattcgtc gccgctgaac atgccgttga cctggccgtg gcaccgatcc aggatgcgca
     2101 acattcgctg cgtatgcccg cggtcgtcct cgtcgcgcga cagccgccat gcgagagtgt
     2161 gcgccttgag cgccatgccc gtattgacca cgtgcttctc ccacgtccaa agcccgcgcc
     2221 ggggcgtcgg cgagagcacg tcctcgccct cgaagaacga cttgtagtcg aacccttgcg
     2281 cccggagctt ccgggccagg tcaagaagcc acgtctcacc ggtctgctca tagacgtaat
     2341 aggccgcgat caagccttcg aaccaccgga accggcccca gttaaacagc ggtgagcggt
     2401 caagaacgcc atgaaccgcc ttcagattgc tgaccacggc gcgcaagacg gcgtcgtcgc
     2461 cggtcgcctc gtggtattgg acgagcactt tgttaacgag caggatggcc cacagatcgt
     2521 acgccttggc ggacgcatcg accggatagg gggcgtacca gccatccgga cgctgcccat
     2581 cgaggatttg cttgacgcgg cgccttgcct tggcctgaag cccttcatca ccgagagtcc
     2641 aggccaacgg aatgaggccg tcgagccaat aaggcgccct ctcccagccc tcagctttcc
     2701 caccgaacca ctggctttgc gcgacgtcgg gccagaactc atccaggtgc ccgctcaagc
     2761 cattcgcctg caattggagt tgacgccgca gccaacccgc gggctcgatc gatcccagcg
     2821 gcagcggccg gaaggccggc tcggccaggc cggaggcggc agcgcccttg gcggaagatg
     2881 gcgcggcgat cccgacgcgg ctcagcggcg cggcagccag cgtggcggtt ccaagggaga
     2941 ctcgggcgag aaacgtgcgg cgttggatcg gtgatttagg catggatcga gggatctgat
     3001 ggtttggctg cgccaatcgc gtgtgtgcgg tcggcttcgg tctccgctcg attgtccatc
     3061 ttcaaaaagg agaatcggcg gcgatacaag ttcacggggc atttgtaccg ggagagcgcg
     3121 gcgtttgaca atgccgaagg ctcgggtgtc tcttcccgca tccgatccgc ggggccaggt
     3181 gggctttctc tcggcagttc tcattttggc ctgggcctcc ggcaagaacc ggctgaagcc
     3241 gggacaccca actacgtccg acgcttcggc gcggtgttcg gtgtgccgcc ttcaggcggg
     3301 tcgtagccaa aatgagaacc gcttgctttc tgtttggcgt tcttgacact ccgggctgca
     3361 cctgctcgcc tcgggggttg tgacgcgcgg ttgcaacgca gaacgtccga ttcagacaga
     3421 cgcgccctgg ccggcgaccg gcctggcggg cggagcgccg agcacgcagc gagagagaga
     3481 gaatccacgc gtgctcgaga tcgagaatct gcgcatcgag ttcgagaccg gcgcccagcc
     3541 cgtgccggtc ttggacggcg tctcgctcga catcgggtcc ggcgagacgg tctgtctggt
     3601 cggcgaaagc ggctccggca agactgtcac ggccctcgcc attgccaggc tcctgccttg
     3661 tccgcccgcc cgctatgccg gtggcagaat ccttgtgaac cgacgggatg cgctgcggat
     3721 gtcggccagg gaactgcgga ctattcgggg gcgcgtcgtc agctacgtgt ttcaggatcc
     3781 ggccgttgcg ctgaacccgg tcctgcgcgc cggggcgcaa gtccttgaaa cactcgctct
     3841 gcatcgtccc gaggcggctc acaccgacga agtcatcagc cttctccgct gggtcggcat
     3901 ccctgcgccc gaattgcgca tccgcgacta tccgcatcag ctctcgggag ggatgcagca
     3961 acgattgatg ctggcgatgg cgttggcatc gcatccgcgg cttctggtgg cggacgaacc
     4021 gaccaccgcg ctcgatgtga cggtccaggc gcagattctc gaactgctgc gcgcactgcg
     4081 tcggcaattg aacatgagca tcctgctcat cacgcacaac ctgggcctgg tcggggagat
     4141 cgccgatcgc gtcgttgtgc tctatgccgg tcaaattatc gaggaggcgc cggccaggga
     4201 gttgctgagc catccgcggc atccttacac tcgcgcgctg atgaagtcgg tgcccgagtt
     4261 gggccgggac gttccgcgcc tcgaggccat tcccgggagc gttcctcgtc tgggcgactg
     4321 gccggcgggg tgccggtttc atccgcgttg tccggttgcg cggcctggtt gttctcgaga
     4381 ggcgcccgcg ttggcgagcg tggcgccgca ccagcgggtc cggtgtccgt tctgggacca
     4441 cgcgccatga gcctgctttc ggtccgcagt ctcagcgtcc gctttccggt tctccgcagc
     4501 tggtggcggg cccgcgcgct cttcgttcac gcggtagacg atgtgagttt tgagcttgcg
     4561 gcgggcgaga ccgtcggttt ggtcggggag agcgggtgcg gcaagacaac gctcggccgc
     4621 gctgttgtgc gtctgatcga gccgacggca ggcgccatcg agtttggagg gatcgatctg
     4681 gcctccttga gacccgggca actgcgtccg gtccgtcggc gattgcagat gatctttcag
     4741 gatccttatg gttcgttgaa tccgtgtctg accgtcgagc agatcatcgg cgaagccttg
     4801 gacattcacg ggctggcccg gacccgtgag gccctgcggg cgcgggtagg cttccttctc
     4861 gatcatgtgg ggctggctcc cgccgacgca cggcgctacc cacgcgagtt cagcggaggg
     4921 cagcgtcagc ggattggcat cgcccgcgcc ctcgctgccg agcctcagtt gatcgtgtgt
     4981 gacgagccgg tcagcgcgct ggacgtgtcg gttcaggcgc agatcgtgaa tctgctgcac
     5041 gacttgcagc gggagcacgg gctggcgtac ctgttcatcg cccacgatct cgcgctgatc
     5101 gaacacctca gccggcgaat cctcgtcatg tatctcggcc gcgtggtcga agccggcgac
     5161 accaagatga tctgccgcac accggcacac ccttacacgc aggccctgat ttctgccgtt
     5221 ccacgactcg atccggtcgc gaatcgacgc cgcattgtgc tcaccggcga ggttccgtcc
     5281 ccgatcactc ccccttccgg ttgcggcttt catccccgct gcccgatcgc tgagccccgg
     5341 tgcggacgcg aggtccctgc cctacgcgag attgcttcgg gccgctgggc ggcgtgccac
     5401 ctggcatggg gcggaaatcg aatggcgacg acaggaggcg tcgtgtaatg tgtggggcca
     5461 tgatatcgag atttcgggaa tcggacgcaa agggtcgttg gggcgaggga agtcggctga
     5521 cggttgcgag cgccgctgtt ctatggctgg cgctgggcat tgcaggaagt gggcgcgcgg
     5581 atgtttggat caacgagttt gtcgcggaca acgaaaatgg cctgcgcacc caggcgggtg
     5641 tggcggcgga ctggatcgag ctggccaacg acagttccca gcctgtcgat ctgagcggct
     5701 ggcatctcac ggatcgggct gatgccccga ccaagtggcg catcccggac ggcacgatca
     5761 tcccggcgaa cggttatctc gttgtgtttg ccgacagttc cgcgcattcg atcaccaaca
     5821 acgaactgca cgccaacttc agcctgtcgg caaatggcga gtatctgggg ttgattcgac
     5881 ctgatggcgt caccgtcgcg cacgaattcg caccggcctt tccgccgcaa ctccgcgatg
     5941 tctcgtatgg gcgcggcacc ccgcgcgaga gggagtggat cggccccagc accccggcgc
     6001 gctatcggat tcccaacgcc gggggaaccg cgccctggac agacgcggtg ggcgccctgg
     6061 gtttcagcga gacgaagggg gcattcaccg tgcgctacta cgaaatgcga aacgacattc
     6121 agaacgtcga tgccgccgag gcgatggtcg ccgacagcag atcatggaag accgacgcag
     6181 catatcccat cgtcggccag tacgacacca tcgacttcca cgcgaactcg gcctccggct
     6241 tcttcacgga gaacgtcctt ttcccgaacc actccttcgc cgggcaggac aaggactgtt
     6301 tcgtgcttgt ggcggaaggg gcgatccacg tgccggctcc gggcctgtgg acatttgccg
     6361 tgggaagcga cgacggcttc cgcctgcgaa tcagcggaca cggcgtcgac tttgtatccg
     6421 agtacctcac ggggcgcagt tttgacacga cgctcgccac gttcaatttt cccgtggccg
     6481 gcgtttacga tttgcgcctc atttactacg agaaccacgg cggggcgagc gtcgagttct
     6541 cggttgcaca ggatttccag gcgagcctcg tgcctgggct ctttcacctg accggcgatc
     6601 ccgccggggg gatcctgcac gcgggggcca ttggatcgtt cgtgcagacg gacatcggcc
     6661 cggcgatgcg gggcgtgaac gcccgcgtcg acgccgagtg gctcttcacg atggacgaat
     6721 cgcccggtct cgacgacaga ttcacgctgt cggtgcggag cgcggacggt ttcgtggcct
     6781 cgctcaacgg ggtgacgatt ggcgcgctga atgcgcccga caacctggtc tggaacagta
     6841 cggcggcgtc agctcgaccg ctcgaagagg cggttcaatg ggtgacgtat gacgtgcccg
     6901 tgtcgagtgt gtcggcgggc accaacatcc tcgcgatcag cgggctgaac gactcggtcg
     6961 acgatccgaa cttcctgatt gcgccgcgga ttgcgagccg cagcgcggag agtatcgcgg
     7021 cttacttcaa ggagccaacg ccgggcgctg ccaacggcag ggcctacgcg ccgcccacac
     7081 cggtcgtgac ggccagcgag ccgcgcggct acaagacgag ccccttcacc gtgtcgctcg
     7141 tctgctcgaa caccacagcc gagattcgct attcgctcga tggcagcgtg ccggacacga
     7201 attccctgct ctatgccggc ccgatcagtg tatccaagac aacgacgctt cgcgcggcgg
     7261 ttgtggattc ggacagcgtg cggcagaacg tgaccacagt gacgtggctt ttcctcgagg
     7321 acattctcga gcagggctcg acgccgccgc cgggttggcc ggccagccgg caggtcaaca
     7381 accacgtcat ggagtatggc ctgcgtcgtg agatcgtgac gagagattcc gcccgcctgc
     7441 gaaacggaat gaccaacgcc attgcctcga tctcgctggt gaccgacctc gacaatctgt
     7501 tcagctctca acgcggcatc tacgtgaacc cgggcaacga cggcatcgcg tgggagcggc
     7561 cggtgtcgat cgaactgatc gatcccgtgc gcgggggcga atcggagttt caaatcgacg
     7621 gcgggctgcg gattcgggga gcgtacagcc gcagttcggg caatccgaag cactcgttcc
     7681 ggctcttctt ccgatcggcc tacggtcagg gcaagctgcg gtttccgctc tttgacgacg
     7741 agggggcgtc cgagttcgac aaagtggatt tgcggacgtc gcagaactac tcgtgggcgt
     7801 acgagaacag caacagggag acgtttgtgc gagagacctt ttcacgcgac tcgcagcggg
     7861 acatggggat gccctacacg cggagccgct attatcatct ctacatcaac gggcagtact
     7921 ggggcctgta tcagacccag gaacgaggcg acgcggacta tgcggagact tacctcggcg
     7981 gcgacgcaga ggactgggac tgcatcaaga ccagccagcc aggctacacg actacggcaa
     8041 gcgacggcac cttcgatgct ttttacgcct tgcacaacat cgccatcaat cagggtttca
     8101 ccggcgccta cgcctccaac tatcaacgcg tcaaaggtct taacccggac ggcacgccca
     8161 atccctcgta cccggtctat ctcgatgagg acaacctgat cgtctacatg ctgacggcgc
     8221 tctataccgg cgatcccgac tcgcccgtct cgatctgggg caatttcccc aacaacctgt
     8281 atggcctgtt caaccggctc catcccgatg gcttcaaatg gcttcgccac gacgccgaac
     8341 actccctcgg ggcgcacggc agctatgggg tcacggccga cacgacgacc gcgggcgcca
     8401 gtttcacgac tcgaggcaat ttcaatcccg ccaccctgca ccagcggctt tgccagcacc
     8461 cccggtatcg agaccgcttc gccgatctgg tccagaagca cctctatggc gacggcgccc
     8521 tgacgccgaa aaacgctcag cagcgctttc gcaaccgcat ggatgagatc gatctggcca
     8581 tcatcgccga gtcggcgcgc tggggacggg gcaagacccg ggatgccacc tggcttccgg
     8641 cctgcaacga ggttctcaac acctacctgg cacagcgccg cgacatcgta atcgcccaat
     8701 tccgcaaccg cggatggttc ccgtcgatcg acgcgcccgg ctactcggtc atgaacagcg
     8761 aggtgcccgc gggcgaggtc gtgcgcctct tgtgcaacac caccttctac tacacaatcg
     8821 atggctcgga tccgcgcctc gaggacggca gcatcaaccc gtccgccatc gaggtcaaat
     8881 ccagtaccgg tcccaccgga cctcggcagg tcgttcggcg cggagccgaa tggcgctatt
     8941 tcgacctcgg cacggaaccg gcgcccgcgg cgcaactcga ctggcgcgac ccggatttcc
     9001 cagatgccga ttgggcgcgg gggccggcca ttctcggcgt tcccggcacc tcacccgcca
     9061 acacggtggc cacgcgcacg cgccgttacg tcaacggggt ggccgcgccc caggtcacaa
     9121 ccacctactt ccgccactcg ttcatcctcg actcgaccaa tgacgccccg aatctgctga
     9181 tcgacatcct gcgcgacgac ggagcgattg tctatctgaa cggcgtcgag attctacgtg
     9241 aggcgatgcc cccgggcccc gtgacttacg ccacgtactc gagcgccgtg gtcggcccgc
     9301 ccgaccagaa cacctacttc agccgctcgg tcaatttcgc ccacctgctg cggccaggcg
     9361 ccaacgtcgt agccgccgag gttcaccaat gcaacgcggg cagttccgac ctttatttcg
     9421 actttgccct gacgatcccg ggccagcagg cgcacgtctt cgcggagatc gcggtgagca
     9481 aagacatgac catcagggcg cgcgccctcg atgggactga gtggagcgcc ctttcggaga
     9541 atgtcctcag agtcgagcgc cctccggtca actacacagc cctgcgcgtc tccgaactca
     9601 tgtacgcgcc tccggcgccg gacgacgaga gtccctacgt cagcgacgac tttgcctgga
     9661 tcgaactttg caatgccggc gcgacgccgc tcgacctcga aggcgtgcga tttgacgacg
     9721 ggatctccca cacgtttgcg ccctgcgtgc tcgaacccgg cgcgcgcctg gtgctggccg
     9781 ccaacccgga cgcttttgcc acgcggcacc cgaccaactc gatccacctt gtcgcctgga
     9841 ccggcggtaa tctggcgcgc ggaggcgaga cgctcgaact ggtggatccg gacggacaca
     9901 cgattctgac attcgcctac tcgaggttct ggtatcctga gaccttcaac acggggcgct
     9961 ccctcgtggc ggtggacctc gccgccgcgg aaccgctctg gagcacccct gacaactggc
    10021 gccccagcag tgcgctgctc ggcacgcccg gcctgcccga gcctcccgtg ttcatcgagg
    10081 cgcgtgtcgc cgacactcga ttgcatgcct ccgcgatcgg aatcgataga acgtccgagc
    10141 tctggttctc ggaagatcta gagacttgga gcctgtgcga ccccgccgcc tggtctcaaa
    10201 caaacggcct cttcaccgtc gacctcgagc atccgtcgct gatcgaccgg acccgatgct
    10261 ttttccagat tcgagtcccg caatgatcag agcagacccg acaacgatgc gatggcttcc
    10321 tggcggaccg acaagaccgc tcgcaggtcg gcctgtgcct tctcgagttg gctttgttgc
    10381 cgacgtcgtg cttcctggta cttggccagc gcgaccttga gttccgcact cgaagccttg
    10441 gcatcgaccg ccttctgcag cgcttcctct tccgcactgg gcgcggggag caggccggca
    10501 agaccccggt tcgcgccggg gcccgcctga ccttcggctc cctcgccacg accgccgcgc
    10561 cggaacattc cggccaacat ccccatcccg ctgaaggcgg tatcccgacg ggcctcgatg
    10621 actttctgga tgcgttcccg gatcacgtcc cattcggcgt cgtcattgat ctcaagtcga
    10681 tcgcgatagc tctgcagcag gaagttctgg atttgagaag ggtcaaaacc gcgccagccg
    10741 gctccaggcg ctccgccccc cgtgccggag gcaggctgag caaacgttgt ccccggcgcc
    10801 caccagcaga cgaccgtcaa tacgagggcg gcgagaacga atcgaggcac aacgctgggt
    10861 gttttcatgg ccgcactatg ggtcggaaca tggagaatgt cgagcccgac gtcgagccgc
    10921 atggacactt ggtgcgacca ggcgcgcgca gtgcgccctg atcggagact ttccatttcc
    10981 caatcaaggc gcggttttgc ttttctaacc cgcattgcag ttatcatgcg aggtctgatc
    11041 gccctgtgca ccgacgggtt gcgaaagatg ggttagccag catttctcag ttcaggacgc
    11101 cgggtggaac tcgacctcga gccgcagagc agtcgatggc ccggcgttgt gcgcggcgaa
    11161 cgggttcgcg gcttggctcg gcacggcgac cacccggcgc ccggacgcac tgtccgaaag
    11221 gccgcgcacc tgaataacgg agaccttgcc agcggccaag gtcgcccggg cggggagaca
    11281 gaggtcggac gatttgggta tgaagacaat ccaggcctcc tcagccagcc atgagttgtc
    11341 cgtgcgcccg gatgcgaatc tcagaggacc gggcttcttg cacaccgggg cctcggagcc
    11401 gttgacatcc ggcggactgg cgtggaggaa actaccacca gaaactgggc gtgtcgtagg
    11461 tgcgaatgac cattctcggg aacttccgcc actcggcatg accgtcgaga cagaggatgt
    11521 tgccgcccgc cggtatccgc ccgttgaggt gtgccgcccg atggtgcttg gtccagccgc
    11581 cgtcgacgct ggtgtagttg tttctcgatc gatccgcctg attgctgccg ctcgagaggg
    11641 tgccgtcagc ggcgatgact cgctcgctgg ccccggggtt gtagagcgat ccatcgctca
    11701 ttctccaggg cgctggattc agcgactcgg tgatgttggt ggcaagaagc cgccccgacc
    11761 ctttgaaggc aacggcgtac ccgatgaccc ggtaaccacc gacgccgtcg tcagccagtt
    11821 cgttggtgac gccggtcgag aagctccaga gttcgtcgtt gtcctgttgc gaaaagcccg
    11881 ggcagtagag gattttccgg ttgccgccgt tgcgaacaaa cgcattggcc gcgggaaccg
    11941 gcaggtccca gggccaatag gcgccggcac agtccgggaa ctgatcgcgg tagtcgttcg
    12001 cgtacatgag gatcgctatc ccgagctggc gcatgttgct ggtgcacttg atctggattg
    12061 ctttggactt cgccttggac agtgcaggaa gcagcatcga ggcgagaatc gcgatgatcg
    12121 caatcaccac cagcaattcg atgagcgtga atgcgcgccc gcgccgggcc tcgagggccc
    12181 gggcggaatt gggcagggat gagacggaac gcatgggcgg agggcggttg aggttcaggt
    12241 cttgaggctg cagaattggc ggtgcggcga ctccttgacg ggctcgcagg gatcgatgat
    12301 ggcgattcgc cgtgtgcgct cggtcatcac aatagtttct aaattctaaa tgcaaccaag
    12361 tatgtccacc acagaccccc ggtcaacccc cgattccgcc cgcctcgcca gcgttccacg
    12421 gttatttcat ttaaattcct gaagggttgc tgttgttgag gttagaaata
//
LOCUS       DAOQRO010000058        12202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4335_length_12202_cov_5.007574, whole genome shotgun sequence.
ACCESSION   DAOQRO010000058 DAOQRO010000000
VERSION     DAOQRO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4335_length_12202_cov_5.007574"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..372
                     /locus_tag="PKK20_03565"
     CDS             115..372
                     /locus_tag="PKK20_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU98996.1"
                     /translation="MNAKQLSKIVFIIVLLFLLVLIGLNNKQMVGFTLPPIITKSVQQ
                     PAAIMYFAFFAVGVLAGAVLTTGTGLKGGGRGGGGTKSSKS"
     gene            complement(450..1349)
                     /locus_tag="PKK20_03570"
     CDS             complement(450..1349)
                     /locus_tag="PKK20_03570"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNU98997.1"
                     /translation="MGNCDFVDLAAWAKTKSLRLSWVSTNSIVGLTNRSNRLRGSVGS
                     RQLEINGVKTWLSSPITSRNGLLYITTLDMRTLLDPILVPVRNKPGQRVRVIALDPGH
                     GGRDPGFKVGKEQEKVLTLLWARRLKARLEQAGFEVILTRARDVYIGLDDRVAAAWRA
                     RADLFISLHYNAASEGSAAVKGVETYCLTPAGAPSTNVQPEQQGSTAKLPGNTQDTHN
                     ILLAYHVQKSMVTRMRVEDRGVRRARFVVLRNTDMPAILVEGGFLSTPDEARRIADPT
                     QRDLLAKAVVEGVLAYKRLVERR"
     gene            complement(1553..2662)
                     /locus_tag="PKK20_03575"
     CDS             complement(1553..2662)
                     /locus_tag="PKK20_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNU98998.1"
                     /translation="MTDPRYPQLARLLVEYSTALEKGDRVLLDMIDVPDEMVVALMRA
                     ARQVGAVPVVEVRHTRVTREVMRETSPDHARLLRDIELFRMRKMQAYIVIRGAANANE
                     AADVRSDRLQLFSKTLRPVLDYRLSKTRWVVLRWPTPSMAQAANMSTEAFEDFYFDVC
                     TANYPRMARAMVPLAKRMQCADQVRLKGPGTDLVFSIKGIGAKPCNGLRNIPDGECFS
                     CPTLKSANGVIQFNTPTLYAGTRFENIRLELKEGRIVKATGSNEKKLNEILDTDPGAR
                     RIGEFSLGFHPRILNPMCDILFDEKIAGSLHFTPGQAYEDCGNGNKSAVHWDMVLIQR
                     PEWGGGEVWFDGELIRKDGLFLPKDLRPLNPDRLK"
     gene            3543..4598
                     /locus_tag="PKK20_03580"
     CDS             3543..4598
                     /locus_tag="PKK20_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNU98999.1"
                     /translation="MTNSVLRRGMSFWLAAVVSLAAGAAEWPPFDEVYGVIRSNLVGV
                     AEADLNRAAVEGLIGQLGSKVVLSRGKEALPEAERAKPDLAGRRLFDRTLAYIRVKRV
                     ECGLGELFRAALADLAAPDQVEGLVLDLRFADGADYGEAGRVADPFVKVSCKLLSWGD
                     EAFETTAKTNAFDRPVMVLVNGETRGAAEALAAALRHADVALLIGSVTAGQASLFREF
                     PLTTGQRLRIASAPVRVGEGREISSAGIKPDIEVAVRVEDERLFLDDPYRTIPRSGEE
                     SRQASASVFGRGPDPSQRVNEADLVRMKREGQDLGGGAFPPPRAAVSEAPPVVTDPAL
                     ARALDLLKGLAVLKGRP"
     gene            4691..5665
                     /locus_tag="PKK20_03585"
     CDS             4691..5665
                     /locus_tag="PKK20_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNU99000.1"
                     /translation="MTRRQFISNSASLLGGLSLGTVTASRAAWSAESTPPAGLKRALI
                     LSKPTEASLAALKNAGFDGAEAGILPPSDAEECRAWAEKLGLRIHSVLRGWAEFNSPD
                     AAKVESSLKVTEDALRAAEAYGADAVLLVPCRIGGMKMPRAWEFHLEFDEATGHLREV
                     VAGDNRPYADYIDAHNRAIDASTEAVKRLIPVAEKTGVVIAIENVWNNLWVRPAHFRH
                     FVASFRSPWVRAYYDIGNHVKYAPSEQWILELGSLLAKVHVKDFKLAPADPAGGGEFV
                     NIRDGSVRWPVVRAALDCVNYRDWLTIEGGDLSMEEHRHRLDLIIEGK"
     gene            6073..6528
                     /locus_tag="PKK20_03590"
     CDS             6073..6528
                     /locus_tag="PKK20_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNU99001.1"
                     /translation="MEIVVALLVVGTALVLLETVLPGMIAGLIGFGCLVAGVVLAYVD
                     FGPRTGNWVLFVVVATLITLGVLWVQFFPNSRLGQMFVSRRTIGGLGVEQPHLLDQQG
                     TALTNLRPSGTALINGRRVDVVTEGGMIERGTAVRVIAVEGLRVVVRAA"
     gene            6590..7648
                     /gene="floA"
                     /locus_tag="PKK20_03595"
     CDS             6590..7648
                     /gene="floA"
                     /locus_tag="PKK20_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419766.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNU99002.1"
                     /translation="MNLVMPQFAAPSFIWTGVWVFIGIIGLVLLILLFNFFGIWIRAW
                     VSGAYVGLIELAAMRLRSVPVGLVVDNYINARKSGLDITVDQLNVHFLAGGDVQMVVR
                     ALIAAQKASIHLEFDRACAIDLATKGTGKTVLDAVKTSVNPKVIDCPNPASGKTTIDA
                     VAKDGIAVKVKARVTVRTNLERFVGGATEETIIARVGEGIVTTIGSSNSYKDVLENPD
                     RISKTVLDKALDANTAFEILSIDIADVDVGDNVGAKLQAEQADANKLIAQAQAEIRRA
                     AAVAIEQEMTARTQEMRARVVEAEAQVPLAIAEAFRTGNLGIMDYLRIKNIQADTGMR
                     DSIAHGTPEDSGQKPKSK"
     gene            7690..8421
                     /locus_tag="PKK20_03600"
     CDS             7690..8421
                     /locus_tag="PKK20_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99003.1"
                     /translation="MPCLAASWESLLVFAVIVLLSGLSNWLKQRQARREEEQSRQSRP
                     IARGRDAVPPLLHPLQAPEDTDWERELRRLFGEEPESPPPRPLPAPPPIEPQPPPLHP
                     RPDTVVLPPRREPSLEPVFEPAASARGADEVAAPAVEDLIREGGVIHLPSSQATAALR
                     GVSSILGKTVARTRAESPIEAQVARPMQSRQRVLSPEIARVRAQLRQPRTAREVFLAT
                     VLLGPPRGLEDHTWPVSSTPATFGR"
     gene            complement(8405..10738)
                     /locus_tag="PKK20_03605"
     CDS             complement(8405..10738)
                     /locus_tag="PKK20_03605"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNU99004.1"
                     /translation="MKSYRVCRVGGWALWTLAGWWAAHLCAAEAPQPESPKAAGLASS
                     LDPVKDLITFSSKYTNVSPLPVGAYDGNVASLTAKLLKRYHYDRRGLDSALSERFLER
                     YFNLLDPMRIHFLKSDIAEFGRLRDRLPELTLERGDSSKANEIFARFLQRVDQRVEYV
                     AGLLVENQFDFTGSDRHTPNRKDADWPDTLDSARELWRQHLRYEYLQEKLNKHKPEEI
                     LKTLSARYKRLLRSWGELDNEEVLQVYLTALSQAYDPHSDYMGKAQLENFAISMKLSL
                     FGIGALLRSEDGYCKIESLVPGGPAARSKKIKPNDRIVAVAQKDQEPVDVIDMKLTKV
                     VEMIRGPKETEVTLTIIPADAADPSERKTLTLVRDEIKLEDQEAKAKIVDMPDTGGST
                     VRLGVIDLPSFYATFDLGSSRRIQLASGGAQPELKSTTADVRRLLNKLQSEGVHGIIL
                     DLRRNGGGSLEEAIRLTGLFIKDGPIVQIKDADGDISIESDPDPEVLYDGPLIVLTSR
                     FSASASEILAAALQDYGRALIVGDTSTHGKGTVQTIYELNKFANFRNYNAGALKVTIR
                     KFYRANGWSTQLKGVTPDIILPSVANDLEVGESSQPFPMPWDSIASAMYEKLDRIQPH
                     LDELRRRTEARQSEDREFAYVREDVVRYRDLLTDKSVSLNEAQRIKEKEELEAKQKAR
                     KEERKSRGEAPVTTYEITLKNADLPGLPDPVSPTNQVALAEADAPPPSDAAADEGEED
                     DKSPAVDVHLEETKRILVDLIALLANNPGAATVTARK"
     gene            10992..11777
                     /locus_tag="PKK20_03610"
     CDS             10992..11777
                     /locus_tag="PKK20_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNU99005.1"
                     /translation="MAHTGGINLIVLAAGFATRLYPLTLTRPKPLLPVAHRDRKDGKP
                     MIEHVLDNVACIPGINRIHVVTNGKFIDVFEAWLREYGARLSHLEIKLVNDGVFDAEK
                     RLGAIGDLDFVLRREGIDDDIIVVAGDNLFSEPLHGFGEFCRRKNTPVLGVYDVRDLE
                     EVKKYNTLTLDSEGRLVFFEEKPQAPTSTLTGIALYFYPRHILPLVHQYIAEGNNPDQ
                     PGRLIQWLYPRVPVFTWRIPGVWYDIGCQETLAEANRIFGSVS"
     gene            complement(11874..>12202)
                     /locus_tag="PKK20_03615"
     CDS             complement(11874..>12202)
                     /locus_tag="PKK20_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNU99006.1"
                     /translation="AEDASDEAGWPGRARACFVLGQEEEVRLAKHLLTFGIVLEAVAG
                     DYRPNYLCNYLYELAGRFTAFYECCPVLKADEPHRSSRLALCDLTAKALRQGLAVLGI
                     ETCERM"
BASE COUNT         2200 a         3625 c         3985 g         2392 t
ORIGIN      
        1 cactcagact tcgcaagagg actgccatgc gggttcgaga agcagaacaa caaacgcggc
       61 aaacaactcg ttgacgctca ttgtgcgcgt ttctatgctt tgtgcgacgg atttatgaac
      121 gcgaaacagc tctcgaaaat cgtttttatc atcgtcctgc tgttcttgct ggtgttgatc
      181 gggctcaaca acaagcaaat ggtcggattc acactgccgc cgatcatcac caagtcggtc
      241 cagcagccgg ccgcaatcat gtacttcgct ttctttgcag tcggcgtttt ggcgggcgcc
      301 gtgctgacca ccggcaccgg cctgaaaggc ggaggccggg gaggcggcgg gaccaagagc
      361 agcaagtcat gaaacgaggc tggcgcgcat gagtgcgcct tccgatgaca tcggcgccgc
      421 gtttttcgga aattgcggag aaatgcgcgc tagcggcgtt cgaccaaccg tttgtaggcc
      481 aggacgccct cgacgaccgc cttggccaga aggtcgcgtt gggtcgggtc ggcaattctc
      541 cgggcttcgt cgggcgtgct gagaaagccg ccttcgacaa ggatggccgg catgtcggtg
      601 ttgcggagca cgacgaaccg ggcccggcga acgccgcgat cctcgacgcg catgcgtgtc
      661 accatcgact tctggacgtg ataggccagg aggatgttgt gggtgtcttg cgtgttgcct
      721 ggcagtttcg cggtcgagcc ttgttgctcg ggctggacat tggtcgatgg cgcgcctgcg
      781 ggcgtcaggc agtaggtctc aacgcccttg accgcggcgc ttccctcgct ggccgcattg
      841 tagtgcaggc tgatgaaaag gtcggcccgg gcgcgccagg ccgccgccac acggtcgtcc
      901 agtccgatgt agacatccct ggcacgcgtg aggatgactt cgaagccggc ctgttcgagg
      961 cgtgccttga gccttctggc ccagaggaga gtgaggacct tctcctgctc tttgcccacc
     1021 ttgaaaccgg ggtccctgcc cccatgccct ggatcgaggg cgatcacccg cacccgctgg
     1081 cccggcttgt tcctgaccgg cacgaggatc gggtccaaga gggtgcgcat gtccagcgtc
     1141 gtgatgtaga gcaagccgtt gcgggatgtg atggggctcg atagccaagt cttgaccccg
     1201 ttgatttcga gctgacggga accgacgctg ccccggagtc ggttcgagcg attggtcaag
     1261 cccacgatgc tgttcgtcga aacccaactg agtctcaggg acttggtctt tgcccaggcc
     1321 gcgaggtcga cgaaatcgca gttgcccatc cggagtgtgg gcagacgcgc ggaggccgca
     1381 tcggcggcag cgagcgcaac cggttgtcgg ctctgcgact cagtggcagc gtgggctggc
     1441 ccgataacga ggattcctgc aaggctcaaa gcagccattc gcttcacgag gcgacgcgcc
     1501 acggcggggg gagaccgccg atccaaccgg caggcagaat cgatccgcac tgttacttca
     1561 agcgatcggg gttgagggga cggaggtcct tggggagaaa gagaccgtct ttgcggatca
     1621 actcgccgtc gaaccagacc tcgccgccgc cccactccgg gcgttggatg aggaccatgt
     1681 cccagtggac agccgatttg ttgccgttgc cgcagtcctc gtaggcttgg cccggcgtga
     1741 agtgcagtga accggcgatt ttctcgtcga agaggatgtc acacatcgga ttgaggatgc
     1801 gcggatgaaa gccgagcgag aattcgccga tgcgccgggc gccgggatcg gtgtcgagaa
     1861 tctcgttcag cttcttttcg ttcgagccgg tggccttgac gattctgccc tccttcaatt
     1921 ccaggcggat gttctcgaaa cgagtgccgg cgtagagagt gggggtgttg aactggatga
     1981 cgccgttggc ggacttgagc gtcgggcagg agaaacactc gccgtcgggg atgttgcgca
     2041 atccgttgca gggcttcgca ccgatgcctt tgatgctgaa cacgagatcg gtgcccgggc
     2101 ccttgaggcg cacctgatcg gcgcactgca tccgtttcgc cagcggcacc atcgcgcgtg
     2161 ccatccgggg gtaattggcg gtgcatacgt cgaagtagaa gtcctcgaag gcttccgtgc
     2221 tcatgttggc ggcttgggcc atgctgggcg tcggccagcg tagcaccacc caccgcgtct
     2281 tggacagccg gtaatcgagc accggacgca gggttttcga gaaaagctgg agcctatcac
     2341 tgcgcacatc ggcggcctcg ttagcgttgg cggcgccgcg gatgacgatg taggcctgca
     2401 tcttgcgcat gcgaaacaac tcgatatcgc ggagcaatcg cgcgtgatca ggcgacgtct
     2461 cccgcatcac ttcgcgcgtc acccgcgtgt ggcggacttc gaccaccggc acggctccga
     2521 cctgtcgcgc cgcgcgcatc aacgccacga ccatttcgtc ggggacatcg atcatgtcga
     2581 gaagaacacg gtcgcccttt tcgagggcgg tcgagtactc gaccaaaagt cgagctaatt
     2641 gcgggtagcg agggtctgtc atattcagat cgggcgagca taagcgggcg cgagggcttg
     2701 gtcgagtgtc attctgacat cctgtgtctc accttttgcc tcaaatcgag acacaaagtg
     2761 ggcttttgca agcagctttt ctcccggagg cacggacagg gcgaccgttc cgcgcacgct
     2821 agaccgccga gcggacctgt caggggactg accactctgt atcgcctgtg aggggaatcc
     2881 cggacatgcg gttgcagagt tcgatcatca acacagcatt gtgatccagc aagcagcttt
     2941 taagcaatgt tttacagata ctacagaacg tttcgaacca gacagtcggc gaattgtttc
     3001 tcgaatccgg aattgtggag aacagaattg gaggcagata tgagggctta catcaacgat
     3061 ttggcatgtg gaatgctctt gaaagaccca caatgaaaga accgagatct agcgacagat
     3121 atcgggactg gcccacagtt ccagcaatca ctgctggaat taaaaagtct ccctaagcct
     3181 accttaaggc gctgggttca ccctggcgtt cccgtctaac gacgtcgccc cggcggccgc
     3241 ggttacgcgg ccgccggttc cattttgccc cggtcatcgg atctcgggcc atccgccctt
     3301 ggcttgcagc gccgcagagg gatagacacc gatgctgtcg gaagttttcc actttgacgc
     3361 tttgggctgg tcttgccaaa gggacgggtt aggctgtgga ggtccgtcgc gccatccatg
     3421 gcgccttggg tggtgttggg tcgggggctg cggctggctg gtgtggcggc tcgttcgcag
     3481 aagtcccggc tggccaccca agtcgctttc gatcatgggg gcggttcagg agcacgattt
     3541 gcatgacgaa ctcagttttg cgccgtggga tgagtttctg gctggccgct gttgtctctc
     3601 tggcggcggg tgccgcggag tggcccccgt tcgatgaggt ctacggggtc attcgatcga
     3661 atcttgttgg ggtagccgag gccgatttga atcgtgcggc ggtcgaagga ctgattggcc
     3721 aactgggctc gaaggtggtg ctatcgagag gaaaagaggc cctgccggag gcggagcgag
     3781 ccaagcctga tctggccggc cgccgcctgt ttgaccgaac gctggcttac atacgggtca
     3841 agcgggtcga gtgtggtttg ggtgagcttt tccgcgccgc gctggccgat ctggccgcgc
     3901 ccgatcaggt cgaggggctg gtcctggatc tgcggtttgc ggacggggct gactatgggg
     3961 aagcggggcg tgtggcggat cccttcgtga aggtgtcttg caagctcctg agctgggggg
     4021 acgaggcatt cgagacgacg gcgaagacga acgcgttcga tcgtcccgtc atggtgctgg
     4081 tcaacggtga gacccgaggc gcggcggagg cgctggccgc cgccttgcgc catgcggacg
     4141 tggctttgct cattggatcg gtcactgcgg gccaggcgag cctgttccgg gagttccccc
     4201 tcacgaccgg gcagcgattg cgcattgcat cggcgcccgt tcgggtcggt gagggccgtg
     4261 aaatcagttc ggcggggatc aagcccgata tcgaagtggc cgttcgggtc gaggacgagc
     4321 gtttgttcct ggacgatcca tatcgaacca tcccccggtc tggcgaagaa tcccggcagg
     4381 cgagcgcaag tgtctttggc cgcggaccgg atccgtcgca gcgggtcaac gaggcggatt
     4441 tggtccgcat gaaacgagag ggtcaggatt tgggcggagg agccttccca cccccgcgcg
     4501 cggcggtgtc cgaggccccg ccggtcgtga cggatccggc gcttgcccgc gctctggatc
     4561 ttctcaaggg cttggcggtg ctgaaggggc ggccctgatg cacggatggg cgcttgcgaa
     4621 ctcggttgct cccgcgcggg tccggcggta gggtggtcgc cgacaaacaa cactcaacag
     4681 gcaaacaacc atgacgcgac gacaattcat ctccaactcg gccagtctgc tcggcggtct
     4741 aagtctggga acagttacgg ccagccgtgc cgcctggtcg gccgagtcca ccccgccggc
     4801 cgggctgaag cgggctctca ttctgagcaa gcccaccgag gccagtctgg cagccctcaa
     4861 gaacgccggt tttgacgggg ctgaggccgg catcctcccg ccttcggatg ccgaggaatg
     4921 ccgggcctgg gccgagaagc tgggactgag aattcattcc gtgctgcggg gatgggcgga
     4981 gttcaacagc ccggatgccg cgaaggtgga atcgagtctc aaggtgacgg aggatgcctt
     5041 gcgcgccgcg gaggcttacg gggcggacgc ggtcctgctg gtgccgtgcc ggatcggggg
     5101 catgaagatg ccgcgtgcgt gggagttcca cctcgagttt gatgaggcga cgggccacct
     5161 gcgagaagtg gttgccggcg acaaccgtcc ctatgcggat tacatcgacg cccacaaccg
     5221 ggctatcgac gcctcgaccg aggcggtgaa gcggttgatt ccggtggccg agaagacggg
     5281 cgtggtgatc gcgatcgaga acgtgtggaa caacctgtgg gtgcgtccgg cgcacttccg
     5341 tcatttcgtg gcgtcgtttc gcagtccgtg ggtgagggcc tactacgaca tcggcaacca
     5401 tgtgaaatac gcgccctccg agcagtggat tctcgaactg ggatcgttgc tggccaaggt
     5461 ccacgtgaag gacttcaagc tcgcgcctgc cgaccccgcc ggagggggcg agttcgtcaa
     5521 cattcgcgac gggagtgtgc ggtggccggt ggtgcgagcg gcgctcgact gcgtgaacta
     5581 ccgcgattgg ctcacgatcg aaggcggcga tctctcgatg gaggaacacc ggcatcggct
     5641 cgatctgatt atcgagggca aatagcccgc gtattagccc ttgtttcttg gacttgcgat
     5701 caaggcgaag cggaagactc cagaccgtgg acccatgagg tcgcggattg ctcccgcaac
     5761 ccgcggcggc gaagccggtc gcgatctggc gcgcgtgcgc ggagccgaag tccgccacgg
     5821 tcttttcatt tggagcggtg cttgacgagt atccgaacag aaaggaaacg gacggatcgg
     5881 cattgaacag ttggtggttt gtcgagatca tcgcacgcct actgcgcccc aaaccgcgcc
     5941 aattgataag cactctttcc aacctccaca acagcaaccc ttcaggaatt taaacgaaat
     6001 catcgtgcga agtccgccgc gccggcgcag ttgtctcttg cgccgggggc aacaccgact
     6061 attcttgagc cgatggaaat cgtggttgca ctcctggtcg tgggaacggc actggtgctg
     6121 ctcgagaccg tcctgccggg gatgatcgcg gggctcatcg gatttgggtg cctggtggcg
     6181 ggcgtcgttc tggcctatgt ggatttcggt ccgcggaccg gcaactgggt tttgttcgtg
     6241 gtggtggcga cgctcatcac cctgggagtg ctctgggtgc agttctttcc gaacagccgg
     6301 ctgggtcaga tgttcgtcag ccggcgaacg atcgggggcc tgggggtcga gcagccgcat
     6361 ctcctcgatc agcaggggac ggcgctcacg aacctccggc catccggaac ggcgttgatc
     6421 aatgggcggc gggtggacgt cgttacggaa gggggcatga tcgagcgcgg cactgcggtg
     6481 cgcgtgatcg cggtcgaggg gcttcgcgtc gtggtgcggg cagcgtgacc gagatcgtat
     6541 gtcagtcgaa atcaacccat aaccgagagc aaacagccaa aagccattta tgaatcttgt
     6601 catgccacag ttcgccgcgc cgtctttcat ctggaccggc gtgtgggtct tcatcggcat
     6661 tatcggcctg gtcctgctga ttctgttgtt caatttcttc gggatatgga ttcgcgcctg
     6721 ggtgtcgggg gcttacgtgg gcctgatcga actggccgcg atgcgcttgc gcagcgtccc
     6781 ggtcgggctg gtcgtcgaca actacatcaa cgcgcggaaa tccgggctcg atatcaccgt
     6841 cgatcaactg aacgtccatt tcctggccgg cggcgacgtc cagatggtgg tgcgcgcgct
     6901 gatcgcggct cagaaggcga gcatccacct cgaattcgat cgcgcctgtg cgatcgacct
     6961 cgccaccaag ggcaccggta aaacggtgct tgatgccgtc aagacctcgg tcaacccgaa
     7021 ggtcattgat tgcccaaacc cggcttcagg caagacaacc atcgacgccg tcgccaagga
     7081 cggcatcgcc gtcaaagtca aagcccgagt gacggtccgg accaatctcg agcgttttgt
     7141 gggcggggcg acggaggaga cgattatcgc gcgggtgggc gagggcatcg tgaccaccat
     7201 cggttcgtcc aacagctaca aggacgtgct cgaaaacccg gatcgcattt cgaagacggt
     7261 cctggacaag gctctcgacg cgaacaccgc cttcgagatt ctgtcgatcg acatcgccga
     7321 tgtggatgtg ggtgacaacg tcggcgccaa actgcaggcg gagcaggccg acgccaacaa
     7381 gctgattgcc caggcccagg ccgagattcg ccgcgccgcc gcggtggcga tcgagcagga
     7441 gatgacggcc cggacgcagg agatgcgcgc tcgggtggtc gaggcggagg cccaggtgcc
     7501 tctggcgatc gccgaagcgt tccggactgg aaacctggga atcatggatt accttcgaat
     7561 caagaacatc caggccgaca ccgggatgcg cgacagcatc gcgcatggca cgcccgagga
     7621 ttcgggtcag aaaccgaagt cgaagtgatt gaatgcggag cgaacgctcc gcgcttgcag
     7681 acactcgcca tgccatgcct tgccgccagt tgggagagcc tgctcgtctt cgcggtgatt
     7741 gtgctgctct ccgggctgtc gaactggctc aagcaacgcc aggcccggcg cgaggaagag
     7801 caatcccggc aatcgaggcc catcgctcga ggtagggacg ccgtcccccc tctgctccat
     7861 cctctgcaag cgccggaaga caccgactgg gaaagggaac tgcgccgcct cttcggagaa
     7921 gaacctgagt cgccgccgcc caggccgctc cccgcgccgc ccccgatcga gcctcaaccg
     7981 ccccccctgc atccacggcc cgacactgtt gttctgccgc cgcgccggga gccgtcactc
     8041 gagcccgtat tcgagcccgc cgcttccgct cgcggcgcgg atgaggtcgc cgctcccgct
     8101 gtcgaggact tgatccggga agggggtgtt atccatctcc catcgagcca ggcgacggca
     8161 gccctgcgcg gcgtcagctc cattttggga aagaccgttg cccgtactcg cgccgaatcg
     8221 cccatcgaag ctcaggtggc tcggccgatg caatcgcggc agcgagtttt atccccggag
     8281 attgcccggg tccgggcgca attgcgtcag cccagaacag ctcgggaggt gtttctggcg
     8341 acggtccttc tcggcccgcc tcgaggtctc gaagaccaca cctggccggt ctcttcgact
     8401 cccgctactt tcgggcggtg acggtggcgg cacccgggtt gttcgcgagg agcgcgatca
     8461 gatcgacgag aatgcgcttg gtctcttcga gatggacgtc cacggctggg gatttgtcgt
     8521 cttcctcgcc ctcgtcggct gctgcgtccg acggcggagg agcgtctgct tcggccaggg
     8581 cgacctggtt ggtcgggctg accgggtccg gcaggccggg cagatcggca ttcttgagcg
     8641 tgatttcgta ggttgtgacg ggcgcttcgc cgcgggattt ccgctcctct tttcgggcct
     8701 tctgtttggc ttcgagttct tccttttcct tgatccgctg cgcctcgttc agtgagacgc
     8761 tcttgtccgt aagcagatcg cggtagcgga caacgtcctc gcgcacgtag gcaaattccc
     8821 tgtcttcact ctgcctggcc tcggttcgac ggcggagttc atcgaggtgg ggttggatgc
     8881 ggtccagctt ttcgtacatc gcgctggcga tgctgtccca gggcatgggg aatggctggg
     8941 acgattcgcc cacctcgagg tcgttggcca ctgacggcag gatgatgtcc ggcgtcaccc
     9001 ctttgagctg ggtcgaccag ccattggcgc ggtagaactt gcggatcgtg accttgaggg
     9061 cgccggcgtt gtaattgcgg aagttggcga acttgttcaa ctcgtaaatt gtctgcaccg
     9121 tgcctttgcc gtgggtggac gtgtcgccca cgatcagtgc acggccatag tcctgcagag
     9181 ccgcggcgag aatctccgag gcggaggcgc tgaatcggct ggtgagaacg atcaggggac
     9241 cgtcgtagag cacctcggga tccggatccg actcgatgct gatatcgccg tcggcatcct
     9301 tgatctggac gatcgggccg tccttaatga acagacccgt gaggcggatc gcctcttcga
     9361 gcgagcctcc cccgttgcgg cgcaaatcga ggatgatccc atgaacgccc tcggactgaa
     9421 gtttattgag cagccgtcgc acgtcggctg tggtgctttt cagttccggt tgcgcgccgc
     9481 ccgatgccag ttgaatccgc ctcgaactgc cgagatcgaa cgtggcatag aacgagggca
     9541 gatcgatgac gcccagccgc acagtcgatc cgccggtgtc cggcatgtcc acaatcttcg
     9601 ccttcgcctc ctgatcctcg agcttgatct cgtcgcggac gagcgtcagc gtctttctct
     9661 cggaagggtc ggcagcatcg gccggaatga tcgtcagggt gacctcggtc tccttgggcc
     9721 ctcggatcat ctcgacgacc ttggtgagtt tcatgtcgat gacatcgacg ggctcctgat
     9781 ccttttgggc gacggcgaca atgcggtcgt tgggtttgat cttcttgctg cgcgccgccg
     9841 ggcctcccgg cacgagactt tcgatcttgc agtaaccgtc ctcggaacgg aggagggcgc
     9901 cgatgccgaa gagggacaac ttcatgctga ttgcgaagtt ctccaactgt gccttgccca
     9961 tgtaatccga gtgcggatcg taggcctggc tcaaggccgt caggtaaacc tgcagcacct
    10021 cctcgttgtc gagttccccc caactgcgga gaaggcgttt gtagcgggcc gagagggtct
    10081 tgaggatttc ctccggcttg tgtttgttga gtttctcctg caaatactcg taacgcaggt
    10141 gctggcgcca gagttcgcgg gccgaatcga gagtgtccgg ccagtcggcg tccttgcgat
    10201 tgggcgtgtg ccggtcgctg cccgtgaagt cgaactggtt ctcgacaagc aggcctgcca
    10261 catactcgac gcgctgatcg acccgttgca ggaaacgcgc gaaaatctcg ttcgccttcg
    10321 acgagtcgcc acgttccagc gtcagctccg gcagtcgatc ccgcaaacgc ccgaattcgg
    10381 cgatgtccga cttgagaaag tgaatgcgca tcggatcgag caggttgaaa taccgctcga
    10441 gaaaacgctc gctcagcgcg gaatcgagcc cgcgccggtc gtagtggtag cgcttgagaa
    10501 gctttgccgt cagactcgcc acgttcccgt cgtaggcccc caccggcagg ggactgacat
    10561 tggtgtactt ggacgagaat gtgatcaggt ctttgaccgg atccagggag gaagccagcc
    10621 ccgccgcctt cgggctttcg ggctggggcg cttccgctgc gcagagatgg gcggcccacc
    10681 accctgccag cgtccaaaga gcccaccctc cgaccctgca cactcgatat gacttcatga
    10741 tcttgagatt cacgccatgc atgcgctgag acgcacgctc gcaagcaaga taggcgaatc
    10801 ccgccccaga gcaagcgcgg cctgcaaagg cccagcggtt ctcgccagag gcccaggcca
    10861 aaatgagaac tgccggcaaa ggcgggcgcg caaggatcgg agcatgacca tgaatcactc
    10921 attcgccgag cgaatccctc cttgaattgg ctcgccatat gcaagcaggc gtcggataat
    10981 acgcaacacg catggcgcac actggcggaa tcaacctgat cgtgctggcg gccggcttcg
    11041 ccacacgctt gtatcccttg acactgacgc ggcccaaacc gctgctgccc gtcgcgcacc
    11101 gggatcggaa ggacggcaag ccgatgatcg agcacgtgct cgacaacgtg gcctgcatcc
    11161 cgggcatcaa ccgcatccac gttgtcacca acggcaagtt catcgatgtg ttcgaggcgt
    11221 ggttgcggga gtatggcgcc cggctgtcac acctggaaat caagctggtc aacgacggag
    11281 tcttcgatgc ggaaaagcgt ttgggagcga tcggagatct cgacttcgtg ctgcgccgcg
    11341 agggcattga cgatgacatt atcgtggtgg cgggcgacaa cctcttcagc gaaccgctgc
    11401 atggattcgg cgagttctgt cgccgcaaaa acacgccggt cctgggggtt tacgacgtgc
    11461 gcgacctcga agaggtcaag aagtacaaca ctctcaccct tgattcggaa ggccggcttg
    11521 tctttttcga ggaaaagccc caagcgccga cgagcaccct gacgggtatc gccctctatt
    11581 tctatccccg gcacattctg ccgcttgttc atcagtacat cgccgaaggc aacaaccccg
    11641 accagcccgg ccggcttatc caatggctct atccccgcgt gcctgtcttt acctggcgga
    11701 taccgggtgt ttggtacgac atcggctgcc aggagacgct ggcagaggcg aacaggattt
    11761 tcgggtccgt ctcctgactc gggcgcccgg gcatcgcgcc ccgcgaggcc ttgcggagca
    11821 tcgggactgg agccacggag gacgcgcacc gctgaccgat cggggtcgcg cggtcacatc
    11881 cgttcgcagg tctcgatgcc gaggacggcg aggccctgcc ggagcgcctt ggcggtcaga
    11941 tcgcagaggg ccaatcggct cgatcggtgc ggttcgtcgg ccttgagcac ggggcaacat
    12001 tcgtagaatg cggtgaagcg gccggcgagt tcatagagat aattgcacaa gtagttgggg
    12061 cgatagtctc cggccacggc ttcgagcacg atgccgaaag tcaaaagatg cttcgccagc
    12121 cggacctcct cctcttgccc cagaacgaaa cacgcgcgcg cgcgcccggg ccagccggcc
    12181 tcgtcggacg cgtcttcggc ct
//
LOCUS       DAOQRO010000059        12029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4428_length_12029_cov_5.796309, whole genome shotgun sequence.
ACCESSION   DAOQRO010000059 DAOQRO010000000
VERSION     DAOQRO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4428_length_12029_cov_5.796309"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..524)
                     /gene="ilvN"
                     /locus_tag="PKK20_03620"
     CDS             complement(51..524)
                     /gene="ilvN"
                     /locus_tag="PKK20_03620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNU99007.1"
                     /translation="MRHTISVLVENKFGVLTRVAGLFSGRGFNIDTLNVAPTQDPTMS
                     RMTIVTRGNDATVDQIVKQLEKLVETVKVQDFREGEFVDRELVMIKVGVDSKTRAEVM
                     QITDIFRAKIIDVQPKTLTIEITGDESKVEKFIELMKAFGILELTRTGKVALPRK"
     gene            866..1795
                     /locus_tag="PKK20_03625"
     CDS             866..1795
                     /locus_tag="PKK20_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HNU99008.1"
                     /translation="MSRRLSAIAPDWWDYTTLDEEIIRAAARLTPEKMLRLARPGFKV
                     VFYDTLESFYLAEALEYIDAWRQSTDDNPVGVCGPIGPTEHLPLVAQIVNALDLDLRS
                     AHFWGMDEWYLDGREVPLDHPLSFERADRALCFDHIRKNLRMPDAHLHFPKADTAPYR
                     RSWDGVRCGVMQGGQGDVKHWAFNDPPRRRGKHNDQPPSPAEYRKLATRVVDLHPLTV
                     AQNARTSGGGNIALVPTQALSVGPVETWKAEKVSIWHAGNHDNPFGQRLTAFMIAERI
                     ADAAVPMSLLADHPNVQFNYYRRGIGTCDVEMH"
     gene            1774..2838
                     /locus_tag="PKK20_03630"
     CDS             1774..2838
                     /locus_tag="PKK20_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99009.1"
                     /translation="MRRGDALRSPGSSGSNGSRRDFLRRNLQAGGLLGVVGALTASGL
                     NVRAARGSAENPFAYDVGKFERTDANLIHYAETARFQSPRPEPRRLALGPDGALHMAA
                     GDSVVRLAEGGAVEFEIALAGPAQCLGVARDGLVYVGLRDHIEVFDAGGQRTAVWSPP
                     GSKTWLTGLAVGEDDLFAADSGGRAVLRYDRSGRLLGRIGDRNKERGIPGFVLPSPFL
                     DVEWHRDGLLRVNNPGRHRVELYTASGDLELAWGKPTAAIDGFCGCCNPIHLATLPDG
                     RCVTCEKGLRRVKVYSALGEFESVVAGPESFAQSVPPGVSMAKAALDAAADAQGRIFI
                     LDPLAGEIRVMTRKRGTTAA"
     gene            2835..3404
                     /locus_tag="PKK20_03635"
     CDS             2835..3404
                     /locus_tag="PKK20_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNU99010.1"
                     /translation="MTPQSPGDGQVSRRNFLRNGARVAALTGLGAVVGTLVHRTSSQT
                     TVWQIDPSKCLRCGRCATECVLDQSAVKCVHSYDLCGYCELCTGYFVPEPNALNTGAE
                     NQLCPTGAIQRKYVEDPYYEYTIDEALCIGCGKCVEGCSRFGNGSLYLQVRQHLCLNC
                     NECAIGRACPGDAFRRVQVSQPYLLKTSH"
     gene            3502..4944
                     /locus_tag="PKK20_03640"
     CDS             3502..4944
                     /locus_tag="PKK20_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNU99011.1"
                     /translation="MKLDLETGAEAPPSKCRRAVVSGFEHRGFWLAFLALAVVWICAG
                     TPALAEARFPPPEFESGHVLPLTATPSPRSLAMQYVDVVALLVALGLACYLVHRRRSR
                     RGIIALSLFSLIYFGFYRKGCICAIGAVQNVALALCDASYALPMTVLVFFFAPLVVAL
                     LAGRTFCAAVCPHGALQDLVLVKSVKVPAWLDHGLSVVPFLFLGAGLAFAATGSAFVI
                     CRYDPFVPLFRLTGSATLLTLGGIFVLASLFIGRPYCRFLCPYGGLLRLAALASKWRV
                     RITPDFCTQCRLCEEACPYGAIREPALTPTAPEALAPARRRLGRLLLLLPIALVVGGW
                     VGSKLSIPFSKVHPTVALAERYADPNKPTVMPSPATPDSLALGRAERDAPRLVAAAVD
                     IRRRFVLSGWLFGGWTALVVFAKLIGLSMRPNRTDFEPDRGACVACARCFLACPNERV
                     RLGLLPASAIPSRPEPGLGTGPTAAGTKRD"
     gene            4960..6768
                     /locus_tag="PKK20_03645"
     CDS             4960..6768
                     /locus_tag="PKK20_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU99012.1"
                     /translation="MAATSQPPPVNDLEARRAARLFWITAAIAAVFCIGVISALVIRH
                     VQSKVDDPLESRELIALKARLLETPKDEHLKEQIRALDLRLRQVYFRQIAFQAAGAWL
                     LAAGGALLVWSARQAARFQAQVYCPRLVTDAAERQDRLAVRARQAVIGVGALAGLGLV
                     ALAVSSGTALPERPDQIGKFLARLAGEEDEEASGPMPAAVFAVNWPRFLGPTGNAYAS
                     NGVPLEPWDVATGAGILWRASATNQGFNSPIVWSNRVFLSGAEQQQREVFCYDADSGA
                     LVWRRPVANIPGSPAQLPEVAESTGLAAPSMATDGRRAFVIFGNGDLAAFRFDGTLAW
                     AKHLGVPKNAYGHATSLVPWQDRLLVQLDQGQAEQNRSRLYAFDGATGRIVWERTRAV
                     PESWATPCLADASAGPQIITLGGPWVIAYAAMDGAELWRGEVLMGEIAPSPIYVGGLV
                     LAVSPMDRLVALRPDGQGDVTQSHVAWTAEDNIPDITSPVGNGELVFLATTYGTLTCY
                     DAGSGEKQWEHELEIEINATPAIARNRLLVLGKKGEVLIAEAAREFKEVARFDMGEPI
                     LASPALLSDRLFIRTTKTLFCIGAKAAESEAPEKRP"
     gene            6840..9302
                     /locus_tag="PKK20_03650"
                     /pseudo
     CDS             6840..9302
                     /locus_tag="PKK20_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
     gene            9299..10939
                     /locus_tag="PKK20_03655"
     CDS             9299..10939
                     /locus_tag="PKK20_03655"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU99013.1"
                     /translation="MKDTDTGNARQTSEFEIVIDGCPVTVREGETVLAGARRLGLDIP
                     TLCYLPKCGPLTSCLVCLVKVRAEEGGGGRLVPSCGLEVQPGMVIESETDEVRTARRT
                     ALELLFSDHVGDCLAPCHRLCPLHLNIPVMLHQVETQTWDAATRTVLQALPMPAILGR
                     LCHHPCENGCRRGTHDQPIAIRDVERLVADRDLASSSPFLPPRKPAAGKSVAIIGAGP
                     TGLAAAFFLLREGHGVTIFDRHDKPGGSLRGEVDGGALESATLDAEMNRLRALGAQFS
                     LGIELGRDETLEGLLTRFDAVLVAWGRKEKALASLALATTPTGIRADPLTAATSQARV
                     FAAGAAVRPIKHLVRAMSEGRSAALAVHRSLLGLKAAGEQRLFSSVMGRLSAPELGLF
                     LVGHNPAPRISPAQGVLRGFTALEGRGEAGRCLHCDCRAEGNCQLQHYGELYGVEPGR
                     FRSQRRPFEQHLERGEIVFEPGKCILCGICVRITELAREPLGLTFIGRGFDVRVAAPL
                     DRAIADGLQKVARECVEACPTGALAFKHDLPPPPRESA"
     gene            11048..>12029
                     /locus_tag="PKK20_03660"
     CDS             11048..>12029
                     /locus_tag="PKK20_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99014.1"
                     /translation="MSDSGLVTTLRSLLCLTLVAGGLAFAFGASPAQNDGDVTAEEAP
                     APAEPGSGDSATPDAPAESEDTPQPEAPEESGEPTEPKENVRPEAALAPKAEEKPPQA
                     EGNNLPNPAPAQPDVTPAPTAGPAQDSPYQSIVLRNAFGLKTPPPPAPPPEQPAQQLS
                     PSALKLSGIITLLGGRHATFVLQEPGKPQINSDLVAEGDTDWLITNLHVLQIDERAGA
                     VRVAYAGKEMTLDFVNNGLKPPAAPAAAPGAVPGRPGQPQPGATTPAATAARATWTRP
                     GAVGIAPSIAPVGSPTLTTPNPSGLQSLPVRPTRLSSGAVSGINAGTQLGG"
BASE COUNT         1915 a         3889 c         4068 g         2157 t
ORIGIN      
        1 gcgtcaaata gaaaggcaaa cccgcgaacc cgtgaccccg ccgtcccgcc tcatttccgc
       61 ggcaaagcca ccttgcccgt ccgcgtcagt tcgaggatgc cgaaggcctt catcagttcg
      121 atgaatttct cgaccttgct ctcgtcgccc gtgatctcga tggtgagcgt cttgggctgg
      181 acgtcgatga tcttggccct gaagatgtcg gtgatctgca tgacctcggc gcgcgtcttc
      241 gagtcgacgc ccaccttgat catgaccagc tcgcgatcca cgaattcgcc ttcccgaaag
      301 tcctgcacct taacggtctc gaccagcttt tcgagctgct tgacgatctg gtccaccgtc
      361 gcgtcgttcc cccgcgtgac gatcgtcatg cgagacatgg tcgggtcttg tgtcggagca
      421 acgttgagcg tgtcgatgtt gaagccgcgt ccgctaaaca gaccggccac gcgcgtcagc
      481 acgccaaact tgttttcgac caacacggaa atggtgtgtc gcatagttca attgctatca
      541 gtcgccccgc tcggcggtca aaacaaatcc agaaaccgct ctttcctcgc acgtgtctcg
      601 aaactgcctc gctcagttcc ttcctcgaaa ggggttcttg attttgtcat attgatctcc
      661 gacccgttgg tgctgacgtg ccaatctgtt aatatcaaga acccgttttc tgttttcgtt
      721 ctccatgagc cgtgcccccc gtgaagggcc gatctcgcga gacggcagag gccgtgaggt
      781 cgagggggga taaacgcctt gaaactgttg cgcgaacgcg gtcaagcggc tagtttacgc
      841 gcaacttggc cataacgtat taaccatgtc acgcagactc agtgccatcg cccccgattg
      901 gtgggattac acgacgctgg atgaggagat cattcgggcc gccgcccggc tcacgccgga
      961 gaagatgctg cggcttgcgc gtccgggctt caaagtggtg ttttacgaca cactcgagag
     1021 cttctacctg gcggaagcgc tcgaatacat cgacgcctgg cgccagtcga ccgacgacaa
     1081 tccggtgggg gtctgcgggc cgatcggccc gacggagcac cttcccctgg tcgcccagat
     1141 cgtcaatgcc ctcgacctcg acctccgatc cgcgcatttc tggggcatgg acgaatggta
     1201 cctcgacggc cgcgaggtgc cgctggacca tccgctctcg ttcgaacggg ccgaccgcgc
     1261 tctctgcttc gatcacattc gcaagaatct ccggatgccg gacgcccacc tccacttccc
     1321 gaaggccgac acggcgccgt atcggcggag ctgggacggc gtgcggtgcg gcgtcatgca
     1381 aggcggccag ggcgacgtga agcactgggc cttcaacgat cctccccggc gtcggggcaa
     1441 gcacaacgac cagccgccgt cgccggcgga gtaccgcaaa ctggccactc gtgtggtgga
     1501 tttgcacccg ttgacggtgg cgcagaatgc ccgcacttcg gggggcggca acatcgcgtt
     1561 ggtgcccacg caagccttga gtgtcggccc ggtcgagaca tggaaggcgg agaaagtgtc
     1621 gatctggcat gccggcaacc acgacaatcc attcggccag cggctgacgg catttatgat
     1681 cgcggagcgg atcgccgacg cggcggtgcc gatgtccctg ctcgccgacc atccgaacgt
     1741 ccagttcaac tactaccgcc ggggcatcgg gacatgcgac gtggagatgc attgagatcg
     1801 cccgggtctt cgggttcgaa cggctcgcgc cgggatttcc tgcgcaggaa cctgcaggcg
     1861 ggcggtctgc tgggggtcgt gggcgcgttg accgcgtcgg gactcaacgt ccgtgcggcc
     1921 cggggcagcg ccgagaaccc gttcgcctac gacgtcggca agttcgagcg cacggatgcc
     1981 aatctgattc actacgcgga gacggcccgg ttccaatcgc cccgtcccga accgcggcgg
     2041 cttgcgctgg ggccggatgg cgcgctccac atggccgccg gcgactcggt cgtgcgcctg
     2101 gcggagggcg gcgcggtgga gttcgagatc gcgctcgccg ggccggcgca gtgtctgggt
     2161 gtggccaggg acggccttgt ctacgtcggg ctgcgcgatc atatcgaggt cttcgatgcc
     2221 gggggccagc ggacggctgt ctggagtccg ccagggtcga agacatggct gaccggcttg
     2281 gcggtgggcg aggacgatct ttttgcggcg gattccggcg gtcgggcggt gttgcggtac
     2341 gatcgctcgg gccggcttct ggggcggatc ggcgatcgga acaaggaacg cggcattccc
     2401 ggttttgtgc ttccgagtcc gttcctcgat gtcgagtggc atcgcgacgg tctcctgcgc
     2461 gtgaacaacc ccggcaggca ccgggttgaa ttgtacacgg cgtcgggcga tctcgaactg
     2521 gcgtggggga aaccgaccgc ggcaatcgac ggtttttgcg gctgctgcaa tccgatccat
     2581 ctggcgacgc tgcccgacgg gcgctgcgtg acctgtgaga aagggctgcg gcgcgtgaag
     2641 gtctacagcg ccctgggcga gtttgaatcc gtcgttgccg ggccggagtc gtttgcccag
     2701 agcgtgcctc cgggtgtcag catggcgaag gccgcgctcg acgcggcggc tgatgcgcag
     2761 ggccgcatct tcatactcga tccgctggct ggggaaatcc gcgtgatgac ccgcaagcgc
     2821 ggcacaactg ccgcatgacg ccccaatctc caggggacgg ccaggtgagc cgccgcaatt
     2881 tcctgcgcaa cggggcgcgg gtggcggccc tgacgggcct gggcgcggtg gttgggaccc
     2941 ttgtgcaccg cacgagcagt cagaccaccg tctggcagat cgatccctcg aagtgtttgc
     3001 gctgcgggcg ttgcgcgacc gagtgtgtgc tcgatcagtc ggcggtgaag tgcgtgcatt
     3061 cgtatgatct ttgcggctac tgcgaactgt gcaccggcta tttcgtgccc gaacccaatg
     3121 ccctcaacac cggcgcggag aaccagctct gtccgacggg cgccatccag cgcaagtacg
     3181 tcgaggatcc gtattacgag tataccatcg acgaggcgct gtgcatcgga tgcggcaaat
     3241 gtgtcgaggg gtgcagccgg tttggaaacg gctcgctcta cctccaggtg cgccagcatc
     3301 tctgcctcaa ctgcaacgag tgtgccatcg ggcgggcctg cccgggcgat gccttccgcc
     3361 gcgtgcaggt gagccaaccc tatttgctca agacctcgca ctgagccggg catgagggac
     3421 gacacgctac gaggtctcga aaagggcacc agaaggccgg ctgccggtcc ggacaccgct
     3481 caacgcggaa agccgcgctc gatgaagctc gacctcgaga ccggcgctga agccccgccc
     3541 tcgaaatgcc ggcgcgcagt cgtgagcggc ttcgaacatc gcgggttctg gctcgcattc
     3601 ctcgcgttgg cggttgtctg gatttgcgcc gggacgccgg ccctggccga ggcgcgattc
     3661 cctccgccgg aattcgaaag cgggcatgtg ttgcccctga ccgcaacgcc gagcccgcgc
     3721 tctttggcca tgcagtacgt cgacgtggtg gcgctcctgg tggcgcttgg gttggcgtgt
     3781 tacctggtgc atcggcggcg gtcgcgacga ggcatcattg cgctgtccct gttctcgctg
     3841 atctacttcg gattctaccg caagggatgc atctgcgcga tcggggcggt gcagaacgtg
     3901 gccttggcct tgtgcgatgc gagctacgcg ttgcccatga cggtgctggt tttcttcttt
     3961 gcgcctttgg tggtggcatt gttagccggg cggacgttct gtgccgcggt gtgcccgcat
     4021 ggcgcgttgc aggacttggt cctggtcaag tcggtgaagg tgcccgcctg gctcgatcac
     4081 gggctgagtg tggtgccgtt cctctttctc ggagccggtc tcgcgtttgc ggcgaccggc
     4141 agcgcgtttg tgatttgccg ctatgacccg tttgtgccgc tctttcgact gactgggagc
     4201 gcgacgctgc tgacgttggg cggtattttt gtcctggcga gcctctttat cgggcgtccg
     4261 tactgccggt ttctctgccc gtacggaggg ttgctgcggt tggcggcgct ggcgtcgaaa
     4321 tggcgggtgc gcatcacccc ggacttctgc acccaatgcc gcctctgcga agaggcctgt
     4381 ccctacggtg cgattcgcga gccggcgctg acgccgaccg ccccggaggc cctggcgccg
     4441 gcccggcggc gccttggccg gctgctgctc ctgctgccga tcgctttggt tgtcgggggc
     4501 tgggtggggt ccaagctgag cattccattc tcgaaagtgc acccaaccgt cgcgctggcg
     4561 gagcgctacg ccgatcccaa caagccaacc gtgatgccca gcccggccac gccggacagc
     4621 ctcgcgttgg ggcgcgccga gcgggatgcg ccgcgattgg tcgcggccgc tgtggacatt
     4681 cggcgccgat tcgtcctgtc cggctggctc tttggcggtt ggactgcgct agtggtcttt
     4741 gcgaagctga tcggcttgtc catgcgaccg aatcgaaccg acttcgagcc ggaccggggc
     4801 gcctgcgtgg cgtgcgcgcg ctgtttcctg gcgtgtccca acgagcgggt gcggctcggg
     4861 ctcctgcctg cgtccgccat tccaagccgc cccgagccgg ggcttggaac cggcccgacg
     4921 gcggctggaa cgaaacggga ttgaagcgcc ccgactcgca tggctgcaac gagtcaaccc
     4981 ccgccggtga acgacctcga agcgcgacgc gcggcacgcc ttttttggat tacggcggcg
     5041 atcgccgcgg tcttttgcat cggcgtcatc agcgcactgg tgattcgaca tgttcagtcg
     5101 aaagtggacg atccgctcga gtcgcgggaa ttgatcgcgc tcaaggccag gctgctcgag
     5161 acccccaagg acgagcatct caaggaacag atccgcgccc tggatttgcg attgcgccag
     5221 gtgtatttcc ggcagatcgc ttttcaggct gccggtgcgt ggctcttggc ggcgggcggc
     5281 gcgctgctgg tttggtcggc gcgccaggcg gcccgcttcc aggcccaggt ctactgcccg
     5341 cgcctggtca cggacgccgc ggagcggcag gaccggctcg ccgtgcgcgc gcgacaggcg
     5401 gtgatcggtg tgggtgcctt ggcgggtttg ggtctcgtcg cgttggccgt gtcgagcggg
     5461 acagcccttc ccgagcgccc ggaccagatc gggaagttct tggccaggct cgcgggcgag
     5521 gaggacgagg aggcgtccgg tcccatgccg gcggctgtgt ttgcggtgaa ctggccgcgc
     5581 tttctcggtc cgacgggcaa tgcgtacgcg agcaacggcg tgccgctcga gccgtgggat
     5641 gtggcgacgg gcgcgggcat actgtggcgc gccagcgcca ccaaccaggg attcaactcg
     5701 cccatcgtct ggagcaatcg cgtctttctc tcaggcgccg agcagcagca aagggaggtg
     5761 ttctgctatg acgcggacag cggggcgctc gtctggcggc ggcccgtcgc caacattcca
     5821 ggcagtccag cgcaattgcc cgaggtcgct gagagcacgg gcctggccgc gccgtccatg
     5881 gcgaccgatg gccggcgggc attcgtgatt tttgggaacg gggatttggc ggccttccgt
     5941 ttcgatggca cgctggcgtg ggccaagcat ctgggcgtgc ccaagaacgc gtacggacat
     6001 gccacgtcgc tggtcccctg gcaggaccgc ctgttggtcc aactcgatca aggccaggcc
     6061 gagcagaatc gttcgcgcct ctacgccttc gatggcgcga cggggcgaat cgtgtgggaa
     6121 cggacacgtg cggtgccgga atcgtgggcc acgccgtgcc tcgcggacgc gtcggctggc
     6181 ccgcagatca tcacgcttgg cggcccctgg gtgattgctt acgcggcgat ggacggcgcc
     6241 gagttgtggc gcggcgaggt cctgatgggc gagattgccc cttcgcccat ttacgtcggc
     6301 ggcctggttc tggcagtgag ccctatggac agactggtgg cgctgcgtcc cgacggacag
     6361 ggcgacgtca cccagagcca cgtggcctgg acggccgagg acaacatccc ggacatcacc
     6421 agccctgtcg gcaacggcga gctggtgttc ctggcgacca cgtacggcac cctgacctgt
     6481 tacgacgccg gaagcggcga gaagcaatgg gaacacgaac tcgaaatcga gatcaacgcc
     6541 acccccgcca tcgcccgaaa ccgcctgctc gtcctcggca aaaagggcga ggtcctgatc
     6601 gccgaggcgg cgcgcgaatt caaggaagtg gcccgtttcg acatgggcga acccattctc
     6661 gccagtcccg ctctcctgtc ggaccggctg ttcatccgca ccacgaagac cctgttctgc
     6721 atcggcgcga aggcggcgga atccgaggcg cccgagaaac gaccgtgaaa ccgcccgcct
     6781 cgacgactca cagtgcgccc tcggccggac ccggcctggc gggcaatccg cccggtgcgg
     6841 tggatttggc ctttgtggac gcggcgatcg agcggctcgg gcggacgccc gcggccctga
     6901 tcccgattct gcaggctgtg caggggcatt acggctatct gcccgcgcgc gccctcgagc
     6961 gaattagcga agtcacggcg atcacaccgg cggcgatcac tggcgtgtcg accttctacg
     7021 acatgttccg acacgcgccg gtcgggcggc atctggtccg cgtctgccac ggcacggcct
     7081 gccacgtcac cggctccgag cgggtcgagg acgcgctccg ccggcagttg cggattccgg
     7141 atggagacga tacggatgcg gaccggcaat tcacgatcga gccggtggcg tgtctgggat
     7201 gctgcactct ggcgccggtg gtcaggattg gcgacagcac gttcggccac accacagccg
     7261 agaaggtccc tcaactcctg cgcgatttcc tcgaaggcca gcgggtgctc ggcggcttgg
     7321 cgcggtcgac ggaggatgtc aaggcgcgac ccggccgcgc gatcgaggtc catgtgggcc
     7381 tgggctcgtg ctgcatggcc aaaggcagcg accggctctt ccacgcgctc gaggatgagg
     7441 ccgagcgctg cggcggcggg gtggtcgtta agcgcgtggg ctgtgtgggc atgtgccatc
     7501 gcacgccatt ggtcgaagtg gccgaggcca acgggcggag cacgttttac gcaggggtgc
     7561 aaccggggaa cgccgccgcc ctgctcgagc ggcatctgcg tccgcgcgcc tggctcgcgc
     7621 gcttgagccg gttctgggag aatgccatcg atcaggtgct ggtcgatcgc accgaactcg
     7681 acctggtccg gtcgtgcgcc ctgaacctgc gagagggaag cgtctcggcc ttcctcggca
     7741 aacaggtgca tattgccacc gaacacttcg gcaagctcga tcccctcgac ctcgacgagt
     7801 acctcgcgca tgacggcttc gtcgccatcc gccgctgtct gggcggcttc gacgcgtcgg
     7861 cgggccgcgg acctctcggc gcatcggcgc ctgacaccga cgcggaatcc gccccgggcg
     7921 ctcagcccac gggaagcgcc cttcccccgg agcgtccgcc gctctctccc gaacagatca
     7981 tcgatgccat cgtggcgtca gggcttcgag ggcgcggtgg ggccggctac ccgaccggcc
     8041 agaaatggcg ggtggcccgc cggcagccgg gcgcggtgaa gtatgttgtc gggaacgggg
     8101 acgagggtga tccgggagcg ttcatggacc ggatgatcct cgaatcgttt ccgtatcggg
     8161 tgatcgaggg catggcgatc gcagccgtcg ccgttggcgc gcacgagggg gccttctaca
     8221 tccggcacga gtatccgctg gccgtgcgcc gggtgcgcgc ggcgattctc gaactcgaac
     8281 ggcgcggctg gctgggggac gcgctgtgcg gattcccata tccgcttcga atcacaatca
     8341 aggagggcgc gggggcgttt gtatgcggcg aggagacggc gctgatccag tccgtggaag
     8401 ggcgccgggg catgcccagc ctgcgcccgc cgttcccggc ggaatccggt ttgtggggga
     8461 agccgaccct gatcaacaac gtcgagaccc tggcgatggt gccgtggatc atccggcacg
     8521 gtccggaggc gtttgccgcc atcggcactg acaccagcaa ggggactaag gttttcgccc
     8581 tggccggaag ggtgcgccgg ggcggcctga tcgagattcc gatgggcaca acggtgcgcg
     8641 agattgtcga ggatattggt gggggtgtcg aggcgggatg ccgtttcaag gctgtgcaga
     8701 ttggcgggcc ctcgggggga tgcgtccctg cgcggctggc ggacacgcca gtcgattacg
     8761 agtcgctcca gcgcctgggt gcgatcatgg ggtcgggcgg gctggtggtg ctggacgatt
     8821 ccacctgcat ggtggacatc gcccgctatt tcctccagtt cacccagaat cagtcgtgcg
     8881 gcaagtgcac attctgccgg atcggcacca agcgcatgct cgagattctc gatcgcctct
     8941 gctgcggacg cggccaaagg caggacctgg acgaactcga atcgttggcc gttcagatca
     9001 gtcggggcag cctctgcggc ctgggcaaga ccgcgcccaa cccggtcttg accaccctgc
     9061 gctattatcg ggacgagtac gaggcgcaca tcctaggacg gtgtccggcg ggcaagtgcc
     9121 gggcgctgat caagtatcac attaccgccg actgcaccgg ctgcacgctg tgcgcccagc
     9181 actgtcctgt cgatgccatc cctatcacgc cctacgaacg gcacgtcatc aacctcgatt
     9241 tgtgcacacg ctgcgactcc tgccgccgcg tttgcccgca tcaggccgtg gaggtgacat
     9301 gaaggacacc gacaccggca atgcgcgcca gacgtccgaa ttcgagatcg tgatcgacgg
     9361 atgtccggtg accgtgcgcg aaggggagac cgtgctggcc ggcgcgcgcc gcctgggact
     9421 cgatatcccc acgctctgct acctcccgaa atgcggtccc ttgacctcct gtctggtttg
     9481 tctcgtcaag gtccgcgccg aggagggagg cggagggcgt cttgtgccgt cgtgcgggct
     9541 cgaggtccaa ccgggcatgg tgatcgagag cgagaccgac gaggtgcgga cggcgcggcg
     9601 cacggcgctc gaactgcttt tcagcgatca cgtcggcgat tgtctcgcgc cctgccatcg
     9661 cctttgcccc ttgcacctca acatcccggt gatgcttcat caggtcgaaa ctcagacctg
     9721 ggacgcggca acgagaactg tgctgcaggc gctgccaatg cccgcgatcc tcgggcggct
     9781 ttgccatcat ccctgcgaga acggctgccg ccgcggaacc cacgatcaac ccatcgcgat
     9841 tcgcgacgtc gaacgcctgg tcgccgaccg agacctggcg tcgtcgagtc cgttcttgcc
     9901 gccgcgcaag cccgcggcgg ggaagtcggt ggcgatcatc ggggccggac cgactggcct
     9961 cgccgcggcc ttctttctct tgcgagaggg gcacggcgtc acaatcttcg atcgccatga
    10021 caaaccgggg gggagcctgc gcggcgaggt ggacggcggc gcgttggaat cggcgacgct
    10081 tgacgccgag atgaaccggc tgcgggccct cggcgcccag ttcagcctcg ggatcgagtt
    10141 ggggcgcgac gagacgctcg aaggcctttt gacccgcttt gacgccgtgc ttgtcgcttg
    10201 gggccgaaaa gaaaaggccc tggccagcct ggctctggca accaccccaa ccggcatccg
    10261 ggccgatccc ctcaccgcgg cgacttcgca ggcacgggtc ttcgccgcgg gagccgccgt
    10321 ccgcccgatc aagcacctcg ttcgcgccat gagcgagggc cggtccgcgg ccctcgccgt
    10381 ccatcgcagc ctgctcggac tgaaagctgc cggcgaacaa cggctttttt cgagcgtgat
    10441 gggacggttg agcgcccccg agctggggct tttcctggtc ggacataacc ccgctccccg
    10501 tatctccccg gcgcaaggcg tgctgcgcgg tttcaccgct ctcgaaggcc ggggcgaagc
    10561 gggccgttgt ctgcactgcg actgccgtgc cgaaggaaac tgtcaactgc agcattacgg
    10621 cgagttgtac ggcgtcgagc cgggccggtt tcgttctcaa cgccgcccct tcgaacagca
    10681 tctcgaacgg ggcgagatcg tgttcgagcc gggcaagtgc atcctgtgcg gcatctgcgt
    10741 caggatcacc gagttggcgc gagaaccgct cgggctgaca ttcatcggac gcggatttga
    10801 cgtccgggtg gccgcgccgt tggaccgtgc gattgccgac ggactccaga aagtggcccg
    10861 ggaatgtgtc gaggcctgtc cgacgggcgc cctggccttc aagcatgacc taccacctcc
    10921 cccacgcgaa agcgcctgac ccgcatagca gtcctcttgc agagtctgag tgccctgtgc
    10981 accatagggg tgtgaaatat gcggaccaga cagcaaccaa cactctctga tggtgttcaa
    11041 aaggaccatg agcgatagtg ggctggtgac aacgctgcgc agtttgttgt gcctgacctt
    11101 ggtggcggga ggtttggctt tcgcgttcgg tgcaagcccg gcccagaacg acggtgatgt
    11161 gacggccgag gaagcgcccg ccccggccga accgggatcg ggagattcgg caacaccgga
    11221 tgcccctgcc gaatccgaag acacccctca gcccgaggcg cctgaggaat ccggggaacc
    11281 gacggaacct aaggagaacg tgaggcccga ggctgctcta gcgcccaagg ccgaggagaa
    11341 accgccgcag gccgaaggca ataaccttcc gaacccggcg ccagcccagc ccgacgtgac
    11401 tccggctccg accgccggtc cggcgcagga ctcgccctac cagtcgatcg ttctgcgcaa
    11461 cgcgttcggt ctcaagacgc caccgccccc tgcgcctccg cctgaacagc cggcccagca
    11521 actctcgccc tcggcgctga agttgtccgg gatcatcacc ttgttgggcg ggcgtcacgc
    11581 cactttcgtc cttcaggagc ccggcaagcc gcagatcaat tccgatctgg tcgccgaagg
    11641 tgacacggac tggctgatca cgaatctcca cgtcctccag atcgacgagc gggcaggggc
    11701 ggtccgcgtt gcctacgcag ggaaggagat gactctcgac ttcgtgaaca acgggctcaa
    11761 accaccggcg gcgcccgcag ccgcacccgg ggccgtgcct gggcgtccag gccagccgca
    11821 gcccggggcc acgacacccg ccgcgactgc agcccgggcg acgtggacca gacctggagc
    11881 ggtggggatc gccccctcga tcgcccccgt ggggtccccg actctcacta cgccaaaccc
    11941 gagcggcctg cagtcgcttc ccgtaaggcc gacccgcctg tcttccggag ccgtttcggg
    12001 catcaacgcc gggacgcagt tgggcggcg
//
LOCUS       DAOQRO010000060        11919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4501_length_11919_cov_4.641689, whole genome shotgun sequence.
ACCESSION   DAOQRO010000060 DAOQRO010000000
VERSION     DAOQRO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_11919_cov_4.641689"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..687)
                     /locus_tag="PKK20_03665"
     CDS             complement(31..687)
                     /locus_tag="PKK20_03665"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99015.1"
                     /translation="MKSIDTTTHKRVAARGIAWGSALLAVLGTASILAQGPEEGFVPL
                     FDGQTLDGWVKRGGDAAYRVDNGTIVGECLPSRMNTFLCSEKEFGNFILKLEFKFDVP
                     GNSGVQFRSAARPEGEGRQRVYGYQAEMDPNPKGDTARIYDEGRRGHQHGIIWMDTTA
                     DETLAAARATNKPGDWNALEIQCVGPSIRTWLNGVAVANLFDDQSLNGFFGSLASHAG
                     "
     gene            787..1872
                     /gene="aroC"
                     /locus_tag="PKK20_03670"
     CDS             787..1872
                     /gene="aroC"
                     /locus_tag="PKK20_03670"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNU99016.1"
                     /translation="MGNTFGHLFRITTWGESHGGGVGVVIDGCPPRLALSEADIQPDL
                     DRRRPGQSRIVTPRKESDSVQILSGTFEGKTLGTPISMWVTNEDARPEAYGEMATKFR
                     PSHADYTYQAKYGIRNWQGGGRTSARETVGRVAAGAVAKKVLREQFGVELLAYVKQVH
                     RLVANADPERVTAADVESNIVRCPDSVVAEEMIRLIEKTRQEGNTVGGIVEGVARGVP
                     PGWGEPVFDRLEADLAKAMLSLPATKGFEIGSGFGCVLMTGREHNDPFRMRDGRVRTV
                     TNRSGGVQGGISNGEMIYFRVAFKPVATVMHEQDTVDVHYQNTTLKGRGRHDACVLPR
                     AVPLVEAMTALVLVDHALRHRAQCAGS"
     gene            complement(2458..3363)
                     /locus_tag="PKK20_03675"
     CDS             complement(2458..3363)
                     /locus_tag="PKK20_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99017.1"
                     /translation="MTTFVPATACLLAALCTVASQGAQAFKPAGDGGFEFDTGVLRGR
                     LRAGGQSLGLSSVVHIPTATRLDSSNGLFSHYRVFTRGKRHGGGAWDWPSSAMLRPDG
                     AVEVVFPATPERPIEMQALYRWAGPATLDLETAVRAVSDLEAFESFLASYFSAPFTNS
                     IVCARDAAGPGQMRFVAADKPAGDWQVFPRDDAAVGVIRDGRWQLEPHPVAWVIRPPL
                     AKPLTVRRAPVTGLTAILMAAPTDCFAIATPHESEGHYSTYLSLFGRDLKAGETARAR
                     ARLVILTSPNDAEIVRQYETWEESR"
     gene            complement(3499..4185)
                     /locus_tag="PKK20_03680"
     CDS             complement(3499..4185)
                     /locus_tag="PKK20_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HNU99018.1"
                     /translation="MPKPIDQLLTIMARLRAPDGCPWDREQSHHSLRYHAVEEVYEMI
                     DAIEAGDDQELEEELGDLLLQVVFHCQLGRERGAFDFTTVAQRLVSKLVYRHPHVFGT
                     AKANTAAAVWAQWDRLKRGEKRGTKNERASAMDGIPRHLPALMRAQKLWKKALKAKVA
                     EPLRVSPPRAGSAPRKDGRTPAQVAKALFELAALCQDRGWSAEALLRQEAKRRERAWR
                     SKEVNGVASK"
     gene            complement(4199..5278)
                     /gene="proB"
                     /locus_tag="PKK20_03685"
     CDS             complement(4199..5278)
                     /gene="proB"
                     /locus_tag="PKK20_03685"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNU99019.1"
                     /translation="MRRKMPQDNISRVVVKLGTGILTDSRKQPDLGQMEQLAAQVAEQ
                     REAGRELVLVSSGAVGAGMGVLSFDKRPTNLAELQACAAVGQSRLMASYEKLFARHGL
                     HVAQVLLTHEDLEHHDRHLNARNTLVTLLQRGVIPIINENDAVSVTELKFGDNDRLSA
                     LVACLLPADLLVILTSVDGLIQNYGQPDARLLPTVEKIDDAIVRLGHGTLSATAVGGM
                     TTKIDAGRIVTRSGIPLVIASGRRRGVLARILDGEAEGTMFVPRERKLQGRKRWIAFF
                     HHPRGTLVVDDGARQAVREANKSLLLPGVIRCEGEFRAGDVVRICDVEGTEFARGIPE
                     FAADQVRAKDLPRTVLVHRDNLVVL"
     gene            complement(5531..8458)
                     /locus_tag="PKK20_03690"
     CDS             complement(5531..8458)
                     /locus_tag="PKK20_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99020.1"
                     /translation="MRQALKSLIASLVLASTSSAHPASLPSWDFTKAADRQGWQPTHD
                     LGPITGSAEGMVLSITGSDPYSSGPVRDYPPDTPLWLHIRLKSDQGGMAQIFYFNTHA
                     TEENSIRFAVPGGGQWHDAKVRLPALGPSYRLRFDPPGTSGTCVLNRLRFEPRVAFHP
                     PIWPSPAPPTLGPDAVTLESGDLKLIHGRGGLGEFEVHVAGQRLACGNNRALIGYVHN
                     NQVRWFPYGYGADAVVTIEHQPLSLLADRTIGGAIRARAVCTDPDGARWQIEQAFTLN
                     QAGALGASTRVSVDQDRAVLYLPVFTLLAGLGDFGTNKIQALLAGVEYLDNEPSSSTA
                     DLNPPASDRQVPDILKLTFPLMALAAQERYVGLAWNRDQPNTCAVFDSPDRFFGSQSH
                     LMGLLFPGSDGVNREESSLLPYDTVTLAANRSVTVSALLLGGRGGAVVPAVQQYVRLF
                     GLPPVPEIGMSTGSFFALAARGWLDSQIRDGNRYRHAAPGFSSGPSADAALYMDWLAL
                     KVGDTELAARLRTGAQAALALVSPANYNSAQVGHVTYPLPALIYGAVSDNAVAAQAHG
                     NALLGRFQPDGSVHYQKPANGLDYGRTHWAPDANGLTAAVLESLLADALFSGNPVLRT
                     LGLRHLRALDKFRNTVPRGAQTWEIPLHTPDILASAHLVRVYTIGFELTRDPDFLEQA
                     RYWAWTGVPFVYLTPPTSNPVGVYGTIPVLGATAWVAPNWIGLPVQWCGLVYADALYR
                     FARHDPGGPWKRIADGIAAAGIQHCYPIDDPDFRGLLPDSYNLRSQTRNGPAINPATV
                     LASAVRLLDEQPLYTFAAFNRHGLLVHAPGEITAIEERTDGVRFTVNTWSARSCSVLI
                     NGLTRAPALEINGQPVGLGSPHQFLAVDGRLILQVSGSTTVNLLYPAIARVMIERSAP
                     QSVKVSWPARTSRATLEVAADLAQSSPWFNLPGPILSDGTSAYTLESPDASGKFYRLR
                     VDL"
     gene            complement(8480..9046)
                     /locus_tag="PKK20_03695"
     CDS             complement(8480..9046)
                     /locus_tag="PKK20_03695"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNU99021.1"
                     /translation="MKKSLGPKTLLYPTPVFIVGTYDPRGKPNVMTAAWGGICCSKPP
                     CVAVSLRKATYSHGNIIARRAFTIGIPSEAHVQAVDRVGLVSGREVDKFADVKLTPVR
                     SDLVDAPYVGEFPMVLECRLAHTIELGLHTQFVGEVVDVKVDETAMTNDGNADILKVK
                     PLVFAPDTQAYYGIGGIVAKAFSVGKSA"
     gene            complement(9064..11052)
                     /locus_tag="PKK20_03700"
     CDS             complement(9064..11052)
                     /locus_tag="PKK20_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU99022.1"
                     /translation="MSTDTDKKSMFVTRDGKNVLFTFVLVSSLFLLWGFCNGMIDVMD
                     KHFQEELHLSLSQSAWVQFAHYLGYFLMSLPAGWLAIKLGYKGGIIAGLLLVAAGGFW
                     FIPATHIAQFWAFLTGVCVIASGLTFLETVANPYTTVLGPPRYAATRINLAQSCNGIG
                     WILGPIAGGMFFYAKDASGRSTGAETLWIPYAGVAVVVLVLAVVFFFAPVPDIKAEDD
                     YRMDDSDNSSAAPAQPERTVHFGRAYGLLLANVCVLIGIFGMILWLILNSIGIGPHLV
                     GLASIFPHPSSVTIHPENALGIVLLWVGGIAVIIAAIALTRTVKRLSHHSIWAHEHFS
                     GATLAQFLYVAAQAGIFSFLINYMVSEPPPLPSAWLKSGASKWIEVRTAFTRSDFKDL
                     RALANKISQKADPISALMATNLSPATARTLSQFQEGTATATAFRVALTQDLNSLVLKQ
                     KLYTPERFAGIALSEKTKQLLAPDAPASSSARCNRMLLADAYPEVLGYRDGILGVSNQ
                     GAANLASFGFICFLIGRITGAALLRKYSAHRVVGLYGVLNVGACLLVFFKLGWISVIS
                     VFLSYFFMSIMFPTIFALGIYGLGARAKRASAFIVMGIMGGAVLPKWMGAVADHYDMS
                     RAFVVPMICFLLIGIYGYAWPRLSRAEALHGVGASGGH"
     gene            complement(11257..>11919)
                     /locus_tag="PKK20_03705"
     CDS             complement(11257..>11919)
                     /locus_tag="PKK20_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99023.1"
                     /translation="VDGVSLVAFESKNTLKNDGANAWTKESGLLSIWILGMFTPSPST
                     TIVVPIVPGPESKLGVKVTSDYFGAIPPERLVVREDVIYLSGDGNYRSKIGISPMRSK
                     AVLGSYDADNRVLTVVQFSQPEGVTDYVNSLWKLQDNPYGGDAANSYNDGPPAPGAKP
                     MGPFYELESSSPAAALRPGKSLTHVHRTIHLSGDESALDTVARAALGVSIADIKNGLK
                     AR"
BASE COUNT         2213 a         3550 c         4055 g         2101 t
ORIGIN      
        1 ttcggaaaac caaggggtca ggactgattt tcaccccgca tgggatgcca gtgaaccgaa
       61 aaagccgttg aggctctgat cgtcgaacag gttggcgacc gccacgccgt tgagccaggt
      121 gcgaatcgat gggcccacac actggatttc aagcgcattc cagtcgcccg gcttgttcgt
      181 cgcgcgcgcg gcggccagcg tctcgtccgc ggtggtgtcc atccagatga tgccgtgctg
      241 atgtccgcgc cggccttcat catagatgcg ggcggtgtcc cctttggggt tgggatccat
      301 ctccgcctga tatccataaa cccgttggcg cccttcccct tccggacgag ccgcggaccg
      361 aaactgcacg cctgagttgc cgggaacatc gaacttgaat tcgagcttca ggatgaagtt
      421 gccgaactcc ttctcgctgc acaggaaggt gttcatccgg ctcgggagac actctccgac
      481 gatcgtgccg ttgtccaccc tgtaggccgc gtccccgccg cgcttcaccc agccgtcgag
      541 cgtctgtccg tcaaagagcg gaacaaaccc ctcctcgggc ccctgcgcca ggatcgaggc
      601 cgtccccagg acagccagaa gcgcgcttcc ccatgcaatg ccacgagctg ctactctctt
      661 gtgagtcgtt gtgtcgattg atttcatgat gcaatgagga tgtacgtctc ttcgggtgaa
      721 ctgtcgatgg aaaagcggga agatgtgatg tttgacctgg cggctcgcct gtcgcactct
      781 gacgacatgg gaaacacgtt tggccacctg ttccgtatca cgacatgggg cgagtcccac
      841 ggcggcggtg tgggcgtggt gatcgacggc tgcccgccgc gactcgcgtt gagcgaggcc
      901 gacatccagc ccgatctcga tcggcgccgc ccggggcaat cgcggatcgt gacgccgcgc
      961 aaggagagcg actccgttca aattctctcc ggcaccttcg aggggaagac tctcgggacc
     1021 cccatctcga tgtgggtgac aaacgaggac gcgcgccccg aggcctacgg cgaaatggcc
     1081 acgaagttcc gtccctccca cgcggactac acctaccagg ccaagtatgg aatacgcaac
     1141 tggcagggcg gaggacgcac cagcgcgcgc gagaccgtcg ggcgcgtggc cgccggagcg
     1201 gtggccaaga aagtcctgcg cgagcaattc ggtgtcgagc ttctcgctta cgtgaagcag
     1261 gtccaccgtt tggtggccaa tgcagacccg gagcgcgtca ccgccgcgga tgtcgaatcc
     1321 aacatcgttc gctgcccgga ttccgtggtg gccgaggaga tgatccgcct gatcgagaag
     1381 acgcggcagg agggcaacac ggtgggcgga attgtcgagg gcgtcgcgcg cggagtgccg
     1441 ccgggatggg gcgagccggt gtttgatcgg ctcgaggccg atctggccaa agcgatgctg
     1501 agtctgcccg caaccaaggg attcgagatc ggctcaggat tcggctgtgt cttgatgacg
     1561 gggcgcgagc acaacgaccc gttcaggatg cgggatggca gggtgaggac ggtgacgaac
     1621 cggtcgggcg gcgtgcaagg gggcatctcg aacggggaga tgatctactt ccgggtggcg
     1681 ttcaagcctg tggcgacggt gatgcacgag caggatacgg tggatgtgca ctatcagaac
     1741 acgacgctta agggacgggg gcgccacgat gcgtgcgtcc tgcctcgggc tgtgccgctc
     1801 gtcgaggcga tgacggcgct tgtgctggtc gaccatgccc tgcggcatcg tgcgcagtgc
     1861 gccggctcgt aacgacatgg ggtcacatcg tcctgcttgg gaaaggaaga aggggtcccg
     1921 aaaaacagga atgttgactg catgatcgca tcttcggggc gtgctgggtt cccgtcctgg
     1981 aggcacaacg cacttcggca ttcgtttatc acctaccgcg ttgcggtaac acaggatgtt
     2041 tccaagaccg cgatcgaggc tggaaacagc ccgaaaacgg tcttctcgaa ctatcgcgca
     2101 ctggcgacag cgaaggaagc gaccgtgtgg ttctcgatca tgccggatga gcgagccgtg
     2161 gcctgtcatc gaggatggtc cgggcgtgca tctcgtctac cactcacgaa aaggggttct
     2221 cacaatcgat aatcaagact ccaatcggtc gctggccgtt gcgatcgagc tgcgcctggc
     2281 gaccttccga ttcaggccaa tggtatgtcc gcgaagactc gaggctctgg gacaacgcga
     2341 agtgactcgg gccaggccat ggatcgaggc gattcggacc atcagtcagg gcctcgcgca
     2401 gctcggcccc cgagcgttca tggccggcgc acggacggcg aggccgccgt tggccgatca
     2461 ccgggattct tcccaggtct cgtactgacg gacgatctcc gcatcgttgg gtgaggtgag
     2521 gatgaccaga cgcgcccgcg ctcgagccgt ttcgcccgcc ttgaggtcgc gtccgaaaag
     2581 tgaaaggtag gtcgagtaat gtccctcgct ctcgtgcggc gtcgcgatcg caaagcagtc
     2641 ggtcggcgcg gccatcagaa tggcggtgag gcctgtgacc ggggcgcgtc gaacggtcag
     2701 cggcttggcc agcggcggac gaatcaccca ggcgaccgga tgcggttcga gctgccagcg
     2761 tccatcccgg atcaccccga ccgcggcgtc gtcgcgcggg aagacctgcc aatcgcccgc
     2821 gggcttatcg gcagcgacga agcgcatctg gccaggtcca gcggcatctc gggcgcagac
     2881 gatcgagttg gtgaagggcg cgctgaagta ggaagccaga aacgattcga aggcttcgag
     2941 gtcactgact gcgcggaccg cggtctcgag atcgagtgtg gccgggccgg cccagcgata
     3001 gagggcctgc atctcgatgg gacgttccgg cgtggccgga aacacgacct cgactgcgcc
     3061 gtcggggcgc agcatggcgc tgctgggcca atcccaggca ccgcctccat ggcgtttgcc
     3121 ccgggtgaac acgcggtaat gactgaacag accgttgctg ctgtccaggc gcgtggcagt
     3181 cgggatgtga acgacggagg aaagcccgag cgattgtccg cccgcgcgca agcgtccgcg
     3241 caagacaccg gtgtcgaact cgaatccgcc gtcgccggcg ggtttgaagg cttgggctcc
     3301 ttggctggcg acggtgcaga gcgcggccag cagacaggcg gttgcgggca cgaacgttgt
     3361 cattggatgg agagtgtgct tcgcgccaat cgaaagccaa gcgccgattc gagacgaggc
     3421 ttgggctgcc caatccgtga gccatcaagt cgcggggcct agacgctcgc ccgcatgttg
     3481 cggggtgcta tttgtcaact atttagatgc gaccccattg acctctttgg atcgccatgc
     3541 ccgctcgcgt cgcttcgcct cctgccgcaa cagggcctcg gctgaccagc cgcgatcctg
     3601 gcagagagcg gcgagttcga acaacgcctt ggcaacctgg gccggcgtgc ggccgtcctt
     3661 ccggggagca gaccccgcac ggggtggaga gacgcggagg ggttccgcga ctttcgcttt
     3721 gagcgccttc ttccacagct tttgcgcgcg catgagggcg ggaaggtggc gagggatgcc
     3781 gtccatagcc gaggcgcgtt cgttctttgt gccgcgtttt tcgccccgtt tgaggcgatc
     3841 ccattgggcc cagacggcgg cggcggtgtt tgccttggcg gtgccgaaga cgtgcggatg
     3901 gcgataaacc agtttggaga cgagtctttg ggcgacagtg gtgaagtcga aggcgccgcg
     3961 ttcgcggccc agttgacaat ggaaaacgac ctgcagcagc agatcgccca gttcctcttc
     4021 gagttcctgg tcgtcgccgg cctcgatcgc atcgatcatc tcgtagacct cctcgacggc
     4081 atgatagcgg agggaatggt ggctttgctc gcggtcccag gggcaaccgt cgggggcgcg
     4141 caagcgcgcc atgatggtca gcaactggtc aatcggtttg ggcataggcg gggcggcact
     4201 aaaggacaac caggttgtcg cggtgcacca gcacggtgcg gggtaggtcc ttggcgcgga
     4261 cctggtcggc ggcgaattcg gggatgccac gggcgaattc ggtgccttcc acgtcgcaga
     4321 tgcgcaccac gtcgccggcg cggaactcgc cttcgcagcg gatcaccccc ggcaacagga
     4381 ggctcttgtt ggcctcgcgc acggcctggc gggcaccatc gtccacgacc agagttcccc
     4441 gcgggtgatg gaagaaggcg atccagcgtt tgcggccctg aagcttgcgt tcgcgcggaa
     4501 cgaacatcgt gccttcggcc tcgccatcga gaattctggc cagcacgccg cggcgtcgac
     4561 cgctggcgat taccagcgga atgcccgaac gcgtcacaat gcgtccggca tcgatcttgg
     4621 tggtcattcc gcccacggcg gtcgcgctca gggtcccgtg cccgaggcgg acgatcgcgt
     4681 cgtcgatctt ctcgactgtg ggcaggaggc gggcgtcagg ttgtccgtag ttctggatca
     4741 ggccgtcgac ggacgtgagg atgacgagga gatcggccgg gagcaggcaa gcgaccagtg
     4801 ccgagagccg atcgttgtcg ccgaacttga gttccgtgac cgagacggcg tcgttttcgt
     4861 tgataatcgg gatgacgccg cgttgcagga gggtgacgag cgtgtttcgt gcgttgaggt
     4921 gacggtcgtg gtgttcgagg tcttcgtggg tgagcagcac ctgggccacg tgcagcccgt
     4981 gacgggcgaa tagcttttcg tagctggcca tcaggcgcga ctggcccacc gcggcgcagg
     5041 cctgcaattc ggccagattg gtggggcgct tgtcgaagct cagcaccccc atgcccgcgc
     5101 cgaccgcccc cgaactgacc aataccaact cgcggccggc ttcccgctgc tcggccacct
     5161 gggccgccag ttgctccatt tgcccgaggt cgggctgctt gcggctgtcc gtcaggatgc
     5221 cggttcccag cttcacgacc acgcgactaa tgttgtcttg aggcatcttg cgtcgcacga
     5281 aaactgagta acaaaccgcc gttcgggtgt cgagggacga aaacgaatgg gcaccgcgga
     5341 gttcacggtg tgtgtgcgat gccgagagaa ccgcgaaatc gcgaaacacg cgaaagggtg
     5401 gcaaacacta atcctcgcct ccaagggcgc tacgagcgca tcggcgtgat ggcagacagc
     5461 gcttcgtctg ggaagccctc tgttcggtcc tggctgtgtc ggatcgtgcc tgcgtttcct
     5521 caacagaggt tcacaagtcc acccgaagcc gatagaactt tcccgaggca tccggcgatt
     5581 cgagggtata cgccgaggtc ccgtcactca ggatcggccc gggcagattg aaccaagggc
     5641 tcgattgcgc aagatcagcc gcgacttcga gggttgcccg gcttgttctg gccggccagg
     5701 acaccttgac cgattgtggg gccgagcgct cgatcatgac ccgcgcaatg gcgggataga
     5761 ggaggttgac cgtggtcgag cctgagacct gcagaatcag acgcccgtcg actgccagga
     5821 attgatgcgg gctgcccagg ccgaccggtt gtccgttgat ctcgagggcg ggggcgcgtg
     5881 tcaggccgtt gatcaggacc gagcaggatc gcgcggacca tgtgttgacc gtgaaccgga
     5941 ccccgtcggt cctctcctcg atcgccgtga tctcgccggg ggcgtgcacc aggagaccgt
     6001 ggcggttgaa cgcagcaaac gtgtagagcg gctgttcatc gagcagacgg acggcggatg
     6061 ccagcacggt ggcggggttg atcgcggggc cgttgcgggt ctgggaacgc aggttgtagc
     6121 tgtcgggcag gagaccgcga aagtcggggt cgtcgatcgg atagcaatgc tgaatgccgg
     6181 ccgcggcgat gccgtcggcg attcgcttcc aggggccgcc cggatcgtgg cgggcgaaac
     6241 ggtagagggc gtcggcataa accaggccgc accattgcac gggcagacca atccagttgg
     6301 gcgcaaccca ggccgtcgct ccgagcacgg ggatcgtgcc atagacgccg acagggttgg
     6361 acgtgggcgg agtgagatag acaaagggca cgcctgtcca ggcccaatag cgcgcctgtt
     6421 cgaggaagtc cggatcgcgg gtgagttcga acccgatcgt gtagacgcgg acgagatggg
     6481 ccgaggccag gatgtcgggc gtgtgcagag gtatctccca ggtctgtgcc ccgcgcggaa
     6541 ctgtgttgcg gaacttgtcg agggcacgca ggtggcgcag gcccagggtg cgcaggacgg
     6601 gattgccgga gaagagggcg tcggcgagca gtgactcgag gacggcggcg gtaaggccgt
     6661 tggcgtcggg cgcccagtga gtccggccat agtccaatcc gttggccggt ttctggtaat
     6721 ggaccgaacc atcgggctga aaccggccaa gcagggcgtt tccgtgcgcc tgggccgcca
     6781 cagcgttgtc ggagacggcg ccatagatca gggccgggag cgggtaggtg acatggccga
     6841 cttgggcgct gttgtagttg gcgggcgaca cgagcgccag ggcggcttgc gcaccggtcc
     6901 gaaggcgcgc ggccagttcc gtatcgccca ctttcagagc aagccaatcc atataaaggg
     6961 cggcatcggc gcttggtcct gacgagaagc ccggagcggc atggcgatag cggttgccat
     7021 cgcggatttg cgagtcaagc cagccccgtg cggcgagagc gaagaagctg cccgtgctca
     7081 tcccgatctc gggcacgggc gggagcccga agaggcggac gtactgctgc acggcgggga
     7141 caacggcccc cccgcgcccg ccgaggagaa gagcgctgac cgtgacactg cggttggcgg
     7201 cgagcgtgac cgtgtcgtaa gggagcaggc tgctttcctc gcggttgaca ccgtcggagc
     7261 cagggaagag caggcccatg agatgcgatt ggctgccgaa gaagcggtcg ggtgagtcga
     7321 aaaccgcgca ggtgttgggt tggtctcggt tccatgccaa tcctacgtag cgttcctgcg
     7381 cggcgagagc catgagagga aacgtcagtt tcaggatgtc gggcacctgg cgatccgagg
     7441 cgggcggatt gaggtccgcc gtcgaactgc tcggttcgtt gtcgaggtac tcgacacccg
     7501 ccagaagggc ctgaatcttg tttgtgccga aatcgcccag accggccagc agtgtgaaga
     7561 caggcaggta aagcaccgcc cgatcctggt ccacactgac gcgggtcgag gcgcccagcg
     7621 ctccggcctg gttcagcgtg aacgcctgct cgatctgcca tctggcgccg tccggatccg
     7681 tgcagacagc tcttgcgcga atcgcgccgc caatcgtcct gtcggccagc aggctcagcg
     7741 gttggtgctc gatcgtgacg acagcgtcgg cgccatatcc gtacgggaac cagcgtacct
     7801 ggttgttgtg cacgtagccg atcaaggccc gattgttgcc gcatgccagc cgttggccgg
     7861 caacgtggac ctcgaattca cccaaaccac ctcggccatg aatcagtttg aggtcgcccg
     7921 attccagcgt cacggcatcg ggaccgaggg tgggtggggc aggcgaaggc cagatcggcg
     7981 ggtggaatgc cacgcgcggc tcgaagcgga gtcggttgag cacgcatgtg ccgctggtgc
     8041 cgggcgggtc gaaacgcagg cggtaactcg gccccagggc gggcagccgg actttcgcgt
     8101 cgtgccactg gccgcctccc ggaacggcga accggatcga gttctcttca gtcgcatggg
     8161 tgttgaagta gaaaatctgc gccatgccgc cctgatcgga cttgaggcgg atgtgaagcc
     8221 agagcggggt atcagggggg taatcgcgga ccggaccgct cgaataggga tcgcttcccg
     8281 taatggacag caccattccc tcggcgctgc ctgtgatggg cccgaggtcg tgagttggct
     8341 gccatccctg ccggtcggcg gccttcgtga agtcccacga cggcagcgag gcgggatggg
     8401 cgctcgacgt cgacgcgagg acaagtgagg caatgaggga tttgagggct tggcgcattg
     8461 ggcgcgaggg caaaccggtt caggcgctct tgccgactga gaaggctttg gcgacgatcc
     8521 cgccgattcc gtagtaggcc tgagtgtcag gggcaaacac aagcggcttg accttcagga
     8581 tgtccgcgtt gccgtcgttg gtcatggccg tctcatcgac tttgacgtcg acgacctcgc
     8641 ccacgaactg cgtgtgcaga cccaactcga tcgtgtgggc cagcctgcac tcgaggacca
     8701 tcgggaactc cccaacataa ggggcgtcca ccaggtcgct tcgcaccgga gtgagcttca
     8761 catcggcgaa cttgtcgact tcgcgcccgg agaccaatcc gacgcgatcg acggcctgga
     8821 catgggcttc ggacgggata ccgattgtaa aggctctgcg cgcgatgatg ttgccgtggc
     8881 tgtaggtggc cttgcgcaac gacacggcca cacacggcgg tttcgagcag caaatgcctc
     8941 cccaggcagc ggtcatgacg ttgggcttgc cgcggggatc gtaggttccc acgatgaaca
     9001 ccggcgttgg atacagcagt gtcttgggtc cgagagactt cttcatagaa cgagcctgtt
     9061 cagttagtgt ccgcccgaag cccccacacc gtgcagggcc tccgcacggc tgagcctggg
     9121 ccacgcatag ccgtaaatgc caatgagcag aaagcaaatc atcggcacca caaaggcgcg
     9181 cgacatgtca tagtgatcgg ccacagcccc catccacttg ggcaggaccg cgccgcccat
     9241 aatgcccatg acgataaagg ccgacgcgcg ctttgcccgg gcgcccaatc catagatgcc
     9301 cagggcgaag atggtgggga acatgatgga catgaagaag tagctgagaa agaccgagat
     9361 cacggagatc cagcccagct tgaagaagac cagcaggcag gcccccacgt tcagcacgcc
     9421 ataaagcccc accacgcggt gcgcggagta tttgcgcagc aacgccgccc ctgtaatccg
     9481 tccgatcagg aaacaaatga acccaaacga cgccagattg gccgcgccct ggttgctcac
     9541 cccgaggatg ccgtcccgat aacccagcac ctccggatac gcgtcggcca ggagcattcg
     9601 gttgcaccgc gcggaactgg aagcgggcgc atcgggcgcg agcagttgtt tcgtcttctc
     9661 ggacagggca atgccggcga agcgttccgg ggtgtagagc ttctgtttca gaacgaggct
     9721 gttgagatcc tgcgtgaggg ccacgcgaaa cgccgtcgct gtggccgtgc cttcctggaa
     9781 ctggctcagg gtgcgggccg tcgctggcga gaggttcgtg gccatcaagg cggagatcgg
     9841 atcggccttc tggctgatct tgttcgccag agcgcgaagg tctttgaaat ccgatcgggt
     9901 gaacgcggtg cgcacctcga tccacttgct ggcgccagac ttcagccagg cggagggcaa
     9961 aggcggcggc tcggacacca tgtagttgat gaggaagctg aagatgccgg cctgggcggc
    10021 gacgtagagg aactgggcaa gcgtggcgcc cgagaagtgc tcgtgcgccc agatgctgtg
    10081 gtgcgagagg cgcttgacgg tccgcgtgag ggcgatcgcg gcgatgatca cggcgatccc
    10141 gcctacccac agcagaacga tgcccagggc gttttcggga tggatggtca cgctggacgg
    10201 gtgcggaaag atcgaggcca atccgacaag gtgggggcca atgccaatcg agttgaggat
    10261 cagccagaga atcatgccga agatgccgat caacacgcac acattggcca gcagcaagcc
    10321 ataggcgcgc ccgaaatgga cggtgcgctc tggttgggcg ggagcggcac tcgagttgtc
    10381 ggagtcgtcc atccggtaat cgtcctcggc cttgatgtcg ggaacgggag cgaagaaaaa
    10441 gaccacggcg agcaccagga ccaccaccgc cacgccggcg tagggaatcc agagcgtctc
    10501 agccccggtg cttcggccgg acgcatcctt cgcgtaaaag aacatcccgc cggcaatcgg
    10561 gccgagaatc cagccgatgc cattgcagga ttgggcgagg ttgatgcgcg tggcggcgta
    10621 tcggggcggt cccagcacgg tggtgtaggg attggcgacc gtctcgagga acgtcaggcc
    10681 cgacgcgatc acgcacaccc cggtcaggaa cgcccagaat tgggcgatgt gcgtcgccgg
    10741 gatgaaccag aatccgcctg ccgcgaccag gagcagaccc gcgatgatgc cgcctttgta
    10801 tcccagcttg atcgccagcc atccggcggg cagcgacatc aggaagtacc ccaggtagtg
    10861 ggcgaactgg acccaagccg actgcgagag gctcagatgg agttcctcct ggaaatgctt
    10921 gtccatcacg tcgatcatcc cgttgcagaa tccccagaga agaaacaggg aactgaccag
    10981 aacgaatgtg aacagaacat tcttcccgtc gcgtgtgacg aacatgctct ttttgtcagt
    11041 gtcagtgctc atagattagg cgcagtggtc tggggccgcg cagacgtcga ggttgccacg
    11101 gaccgccacg gcgcgtcccg aaccggctca gattctcatc ttcctgcata gtggccggac
    11161 cgtccgttcg cttgcggctg tccgagtgca atcgtggaaa cgctcgaagt tgacggagtc
    11221 agactcgatc tcggtacgtt ttctcctcgg caataactac cgggccttga gtccgttctt
    11281 gatgtcggcg atggagacgc ccaatgctgc ccgggccacc gtatcgagcg ccgattcgtc
    11341 gccgctcaga tggatggtgc ggtggacgtg cgtgaggctc ttgcccggac gcagcgcggc
    11401 ggctggcgac gaggactcga gctcgtagaa cgggcccatg ggtttggcgc ccggcgcggg
    11461 cgggccgtcg ttgtagctgt tggccgcatc gccgccgtag ggattgtcct gcagtttcca
    11521 gagcgagttg acatagtcgg tgactccctc gggctggctg aattgcacca cggtgagaac
    11581 ccggttgtcc gcgtcatagc tccccagcac agccttgctc cgcatcgggc tgatcccgat
    11641 cttgctccgg taattgccgt cgccgctcag gtagatgaca tcctccctca ccaccagccg
    11701 ctccggcgga atggcgccga agtaatcgga ggtcaccttg accccgagtt tggattccgg
    11761 cccgggcacg atgggaacca cgatggtggt cgagggcgag ggggtgaaca tgccaagaat
    11821 ccaaatcgag agcaggccgc tctcctttgt ccaggcgttc gcgccgtcgt tcttcagcgt
    11881 gttcttggat tcgaacgcca ccagactcac gccatcgac
//
LOCUS       DAOQRO010000061        11859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4544_length_11859_cov_4.662487, whole genome shotgun sequence.
ACCESSION   DAOQRO010000061 DAOQRO010000000
VERSION     DAOQRO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4544_length_11859_cov_4.662487"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2671
                     /locus_tag="PKK20_03710"
     CDS             <1..2671
                     /locus_tag="PKK20_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNU99024.1"
                     /translation="SITIPRGQFVVITGVSGSGKSTLAFDLLFAEGQRRFLDSMNAYA
                     RQFVEQLARPDVDLITGIPPTVSIEQRNSRGGGKSTVATITEIHHFIRLLFARLGVQH
                     CPDCRQPVEPQTRDRVGEALRAEARRRGELSLLAPMVRGRKGFHSEVADWAARHGYER
                     IRVDGQLRSSAQGLRLDRFREHHVEILVGTVRRDGRQATLAGAAATLQKLVDETLRLG
                     QGTLLALDREGRVTPHSIERTCTGCGRSFQPLDPKLFSYNSAAGWCPRCRGFGELFYL
                     PEDVDRGARADAIEESWFGWQEGQRQMCPECGGHRLNPIARAVRLPFDAPNCPTASLR
                     QNPTIDDFGAATVDSVRAYLDSLRVSGRAVLIARDIVSEIRARLWFLHEVGLGYLQLG
                     RAVTTLSGGEAQRIRLAAQLGSNLSGVLYVLDEPTIGLHARDNQRLLAALAKLKARGN
                     SLVVVEHDEATMRSADYIIDLGPGAGINGGRLIASGSLDVLMRHRDSITGQCLRARKT
                     FPARGVRRLVAPPPGGTRPRRKRAPAGAGAPDADAASLPAFLVLEGAAAHNLRNLTVR
                     IPLGRLVLITGVSGSGKSTLVRECLLPAIEANLKSRVSNPKSGARLTGGQQFIRAVHE
                     VDQSPIGRTPRSIPATYVGFFDDIRRLYAQLPEARLRGYGPGRFSFNSPQGRCPECEG
                     AGVVRIEMNFLPPAFVRCESCGGMRFNRETLDITLHGKNVAQVLDLSVSEALEFFAAH
                     PRIHRSLDALRDTGLDYLKLGQTSPTLSGGEAQRVKLVTHLLAGLREREPHPQHTGKR
                     NLFILEEPTIGLHMADVRRLVEVLQRLVDAGHTVLVIEHNLDLIAEADWIIDLGPEGG
                     EGGGRVVAEGTPEQLARLPRSHTGRFLRSHLSN"
     gene            complement(2678..4882)
                     /locus_tag="PKK20_03715"
     CDS             complement(2678..4882)
                     /locus_tag="PKK20_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNU99025.1"
                     /translation="MQSASPATPPESLAGLIERVTFFNEDTGFAVLKVKARGHRDLVC
                     VVGALASVAAGEYIHGEGRWVQDREYGLQFRADVLTSTAPTTREGIEKYLGSGMVKGI
                     GPVYARKLVAKFGEQIFDIIDRFSARLEEVEGIGPKRRHRIKEAWSEQKAVREIMVFL
                     HSHGVSTSRAVRIYRTYGAQAVETVRRDPYCLARDIHGIGFKTADEIARKLGVPHDSI
                     ERACAGLDHVLLTATNDGHCALPNEALKEEAEKLLSVGPAVVDAALDRALAQGNLAQE
                     TIEDQALLFLPRLQRAEEAIANSLCRLAAMPPAFPSIDLDKAVAWCQAKTGTELAPTQ
                     REALRKAWSARVLVVTGGPGVGKTTLVKSILLILRAKRVRCLLCAPTGRAAKRMAEAT
                     GFEAKTIHRLLEAQPGSGGFLRNEANPLDCDYLVVDEMSMVDVPLMHQLLRALPRQAG
                     LLLVGDADQLPSVGPGLVLRHVIESGAIPVARLTEIFRQAAHSQIVITAHRVNEGQLP
                     PPLPPNTASDFYFVERSDPEAILHTVIEMVKRRIPTRFHLNPVGDVQVLCPMNRGLLG
                     MRQLNVRLQAELNPQRPDQAVIERFGWQFRIGDKVMQTENNYDKEVFNGDLGRVERID
                     MIEREMTVRFDQRDVIYDFGELDEVVLAYAITVHKSQGSEFPAVVMPLAMQHYLLLQR
                     NLLYTGITRGKRLVVLVGQANALALAVRNNRTRHRFSGLLARLRREGERYKG"
     gene            5197..6042
                     /locus_tag="PKK20_03720"
     CDS             5197..6042
                     /locus_tag="PKK20_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU99026.1"
                     /translation="MPVVRDHARVFDRFLFVYPVISRRSGGLSLGVNLNPDKRCNFNC
                     AYCEVDRQTPGPAKTVDLIRLRAELSALAQSALRGQLGLTPKFAEVAALTRRIRDIAF
                     SGDGEPTMVPNFADCVRAVIEVKLALALADTKIVLITNATGLDKTDVKRGLALLDAHQ
                     GEIWGKLDAGTDAYYRLVNRSHVAFDRILHNLTITAQARPIVIQSLFMKVRGEPMPAP
                     ELEVYCGRLRDILAAGGSIREVQAYTIARPTPEPWVERLDRADLDSIAAVIRARTGLP
                     VMTFA"
     gene            6068..6814
                     /gene="ispD"
                     /locus_tag="PKK20_03725"
     CDS             6068..6814
                     /gene="ispD"
                     /locus_tag="PKK20_03725"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNU99027.1"
                     /translation="MNAAVIVAAGRGTRMGPEVDKLFLEAAGRPIIAHTWQRFQDADC
                     IHEIVLVVRAGLEKAFPEMATRFGFQKPWRVVTGGAERQDSVWNGLQAVSDQTQVVAI
                     QDGARPCTTAALIRATIDAAQVTGAAVAAQRVTDTIKESDDGVMIVRHPERSRFWAVQ
                     TPQAFRIGVIRRALAAVRERGARVTDDTAACDLIGQRVQLVESRSPNPKITVPDDLAL
                     LEWRLSRMNDPSRTAADRGGSHALREIATI"
     gene            6918..8249
                     /locus_tag="PKK20_03730"
     CDS             6918..8249
                     /locus_tag="PKK20_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNU99028.1"
                     /translation="MTKRLMYSVLLTALAVNLFFGAQVYLRSAEVAEKNDVYGNLRLF
                     SLVLERVRNDYVDGENLTYQDLIYGALKGMLSTLDPHSEFMEPQKYDELKKDTEGKFG
                     GVGIQVGLRGEYLTVIAPMDDTPASRAGIMSGDRILKIDGRSTEKISLTEAVNRMRGA
                     PGTEVVLTVLRPSTGATKDHKLVRADIAVETVKDIHGKREFSVDDDKLGYVRLTQFGE
                     ATGEELDRALKKLHDQKMRGLVLDLRNNPGGLLDQAAHVCERFVQPRQLVVTTEGRDP
                     SERREYRAGRRGERTTVPMVVLVNGGSASASEIVAGCLQDLKRAVVLGEKTFGKGSVQ
                     SIIPLQDGAALRLTTAKYYTPSHKVIHEKGIEPDIVVPVPIEDADALLLQRTPGLIES
                     LDAERQERFGAVRDIQLERALDLLKGITLYTHRAPDSDRRPDTKPMAAKAD"
     gene            8253..9299
                     /gene="tsaD"
                     /locus_tag="PKK20_03735"
     CDS             8253..9299
                     /gene="tsaD"
                     /locus_tag="PKK20_03735"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNU99029.1"
                     /translation="MTLLAIETSCDETGVAVVRNGHVLANLVSSQTSLHAEYGGVVPE
                     LATREHLRNLPVLIQAALCEAGTDPPAIEALAATQGPGLPNALMVGLKAAQGIACALR
                     RPFLGIHHHEAHLYSAWVKGDPPHADFPAIQPHVALIVSGGHTLLIHVPEGLRHRVLG
                     STLDDAAGECFDKTAKLLGLPYPGGPVMDQLAAEGNPAAFPFPRPLLNDAAYDFSFSG
                     LKTSVRYFLRDHPGLADDPQALRDLCASVQAAIVEVLLAKTTRAARDLGVRLVTASGG
                     VTCNRALRRDLASACARDGLDLRLAPTAFCTDNAAMVGLLAEWKLASGVPPDDPDGDV
                     RPAWSLESMPARGG"
     gene            complement(9836..10798)
                     /locus_tag="PKK20_03740"
     CDS             complement(9836..10798)
                     /locus_tag="PKK20_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNU99030.1"
                     /translation="MPTETLHFDNARLAQQLFNNDARNLSALERQLGVKATARDAWIK
                     LEGETEAVTRARHLFQLLEGTLKTGGHVRTQDFAMALSVVRNEGVAALESLQAERIQT
                     SPKKATVTPKTTGQRRYIEAIRQHDITLGVGPAGTGKTYLAMAMATAALRGAKVNRLI
                     LTRPAVEAGEALGFLPGDLQEKLAPYLRPLYDALHDMLPAEEIQRLMERGTIEIAPLA
                     YMRGRTLNNSFIIFDEAQNSTTEQMFMFLTRLGHNSKAVITGDITQIDLPRHKPSGLI
                     EARQALAQVEGIAVIELGKKDVIRHPLVARIVHAYEQHRASQPA"
     gene            complement(10814..11386)
                     /locus_tag="PKK20_03745"
     CDS             complement(10814..11386)
                     /locus_tag="PKK20_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNU99031.1"
                     /translation="MEHLHAPWRIEYIRAPKPPPSDESLFTHIAQAADDVANHVLVRE
                     KSCFAVLNTYPYTGGHLMVVPYRRTADLNDLTEQELTDLMVLTRRCLNALTRAMKPEG
                     FNVGINLGRVAGAGVADHLHIHIVPRWNGDTNFMPVVAQTTVMPEALDDVAARLRAAL
                     AMDKEEGVRTSHAPNAPVIGAGGGEIGNRS"
     gene            11521..>11859
                     /locus_tag="PKK20_03750"
     CDS             11521..>11859
                     /locus_tag="PKK20_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNU99032.1"
                     /translation="MKSVRLFLTLCASLCLLRAQDDNLPTLFVAPFDGDRSEISYWQP
                     ALGEGLAEMLITELSKTGKFQMLESTAIESLKDEIRMGEDGWVAKDERVEKGGWAGAD
                     YMFRGKVTRFG"
BASE COUNT         2089 a         4020 c         3681 g         2069 t
ORIGIN      
        1 ctcgatcacc atcccccgtg gccagttcgt ggtaatcacc ggcgtgagcg gctcgggcaa
       61 gagcacgctc gccttcgatc ttctcttcgc cgagggccag cgccggttcc tcgattccat
      121 gaacgcctac gcccggcagt tcgtcgagca actggcccgc cccgatgtcg acctcatcac
      181 cgggattccc cccacggtca gcatcgaaca gcgcaattcc cgcggcggcg gcaagagcac
      241 cgtcgccacc atcactgaaa tccaccactt catccgcctt cttttcgccc gcctcggtgt
      301 ccagcactgt ccggactgcc gtcagccggt cgagccgcag acgcgcgacc gggtgggcga
      361 ggcgctgcgg gccgaggcgc ggcggcgcgg cgagctctcg ctcctggcgc ccatggtgcg
      421 cggacgcaag ggattccatt ccgaggtcgc cgactgggcc gcgcggcacg gatacgagcg
      481 aatccgggtc gatggccagc ttcgatccag cgcgcaaggg ctccggctcg atcggttccg
      541 cgagcatcac gtcgaaatcc tcgtcggcac cgtccgccgc gacggacgcc aggctacact
      601 ggccggggcg gcggcgactc tgcagaagct ggttgacgag accctgcggc tgggacaggg
      661 cacgttgctg gccctggacc gcgagggccg tgtgacaccg cactcgatcg agcggacctg
      721 cacaggctgc ggccggtcgt ttcagccgct cgatcccaag ctgttcagct acaactccgc
      781 cgccggctgg tgcccacgct gccgcggttt cggggagctg ttctacctcc ccgaggatgt
      841 ggatcgcggc gcgcgggccg acgccatcga agaatcgtgg ttcgggtggc aggaaggcca
      901 gcgccagatg tgccccgagt gcggcggaca tcgcctcaac cccatcgccc gcgccgtgcg
      961 cctccctttt gacgctccga actgtccaac cgcatccctc cgccagaacc ccaccatcga
     1021 cgacttcggc gcggcaacgg tcgacagcgt gcgggcgtat ttagactcct tgcgggtgtc
     1081 cggccgcgcc gttctcatcg ctcgcgacat cgtgtcggag attcgggcgc gcctctggtt
     1141 tctccacgag gttggcttgg gctacctgca attgggccgc gccgtcacga ctctctccgg
     1201 cggggaagcc cagcgcattc gcctcgcagc ccagttgggc tcgaacctca gcggcgtcct
     1261 ctacgtcctc gatgagccca cgatcggcct gcacgctcgc gataaccagc gcctgctcgc
     1321 cgccctggcc aaactcaagg cccgcggcaa ctccctggtc gtggtcgagc atgacgaggc
     1381 cacgatgcgg tccgctgact acatcatcga cctggggccc ggcgccggga tcaacggcgg
     1441 acggctcatc gctagcggct cgctcgatgt gttgatgcgg catcgcgact cgattaccgg
     1501 tcaatgcctg cgcgcccgga agacgttccc cgcccgcggc gtgcggcggt tggtcgcgcc
     1561 accgcccggc ggaacccgtc ccaggcggaa acgcgcgcct gccggcgcgg gtgcgccgga
     1621 cgccgacgcg gcctctctgc ccgcgttcct ggtgcttgaa ggggcggcgg cgcacaacct
     1681 gcgcaacctc accgtgcgga ttccgctcgg acgcctcgtc ctgatcaccg gggtgagcgg
     1741 atcgggcaag agcacgctgg tgcgcgaatg cctgttgccc gcgatcgaag ccaacctcaa
     1801 atcccgcgtc tcgaatccca aatcgggagc ccgattgaca gggggacagc agttcatccg
     1861 cgccgtgcac gaagtggatc aatcgccgat cgggcgcacc ccccgctcga tcccggcaac
     1921 gtacgtgggt tttttcgacg acatccgtcg gctctatgcc cagttgcccg aggcgcggtt
     1981 gcgcgggtac ggcccggggc ggttttcgtt caacagccca cagggtcgct gccccgagtg
     2041 cgagggggcg ggcgtggtca ggatcgagat gaacttcctg ccgccggcat tcgtgcgttg
     2101 cgagagttgc ggcgggatgc ggttcaaccg cgagacgctc gacatcaccc tccacggcaa
     2161 gaacgtcgcc caggtgctcg acctgagcgt ctccgaggcc ctcgagttct tcgccgcaca
     2221 cccgcgcatc caccgttccc tcgacgccct ccgcgacacc ggactcgatt acctgaagct
     2281 cgggcagacc agtccgaccc tcagcggcgg agaggcccag cgggtcaaac tcgtgaccca
     2341 cctgctcgcc ggattgaggg agcgcgaacc gcacccccaa cacaccggaa agcggaatct
     2401 ctttatcctc gaagaaccca ccatcggatt gcacatggcg gatgtgcgtc ggctggtcga
     2461 ggtgctccaa cgcctcgtgg acgccggcca taccgttctc gtgatcgagc acaatctcga
     2521 cctcatcgcc gaagccgact ggatcattga cctggggccc gagggcggcg agggaggcgg
     2581 acgcgtggtg gccgaaggca cgcccgagca gttggcccgc cttccgcgct ctcataccgg
     2641 ccgtttcctg cggagccacc tctcaaactg acgaacgtta ccctttgtaa cgctcgccct
     2701 cccgtcgcag acgcgccagc aaccccgaga atcgatgccg ggtgcggttg ttccgcacgg
     2761 ccaacgccag ggcgttggcc tgccccacca gcaccacaag ccgtttcccg cgcgtgatcc
     2821 cagtgtagag caggttccgt tgcagcagca ggtaatgctg catggccagc ggcatcacca
     2881 cggccggaaa ctccgatccc tgcgacttgt ggacggtgat ggcatacgcc agcacgactt
     2941 cgtccagctc tccaaaatcg tagatcacat cccgctggtc gaatcgcacc gtcatctcgc
     3001 gctcgatcat gtcgatccgc tcgacccgcc ccagatcccc gttaaacacc tccttgtcgt
     3061 agttgttctc cgtctgcatc accttgtccc caatccggaa ttgccagccg aatctctcga
     3121 tcaccgcctg gtccggccgc tgcggattga gctcggcctg gagtcgcacg ttgagctgcc
     3181 gcatccccaa caacccgcgg ttcatgggac aaagcacctg cacatccccg accggattta
     3241 gatgaaatcg cgtcgggatg cgccgtttca ccatctcgat cacagtgtgc agaatggcct
     3301 ccgggtcgct gcgctccaca aaatagaaat ccgaggcggt attgggcggc agcggcggcg
     3361 ggagttggcc ttcgttgacg cgatgggccg tgatgacgat ctggctgtgg gcggcttggc
     3421 ggaaaatctc ggtcaaccgg gctaccggga tcgccccgct ctcgatcaca tggcgcaaca
     3481 ccagacccgg gccgaccgat ggcagttggt ccgcgtcgcc caccagcagc aggccggcct
     3541 gacgcggcag ggcgcgcagc aactggtgca tcagcggcac gtccaccatc gacatctcgt
     3601 cgacgaccag atagtcgcaa tcgagcggat tggcctcgtt acgcaggaac ccgcccgagc
     3661 ccggctgcgc ctcgagcagg cggtgaatgg tcttggcctc gaatcccgtg gcctccgcca
     3721 tccgttttgc ggcccgcccc gtgggcgcgc acaacaggca acgcactcgt ttggcccgca
     3781 gaatgagcag tatcgattta accagcgtcg ttttgcctac acccggtccg cccgtgacaa
     3841 ccaacacgcg ggcggaccag gccttgcgca gcgcctcccg ctgtgtagga gccagttccg
     3901 ttccggtctt cgcctggcac caagcgacgg ctttgtccag atcgatgctc gggaatgcag
     3961 gtggcatggc cgcaaggcgg cagagtgagt tggcgatcgc ctcttccgcc ctttgcagcc
     4021 ggggaaggaa cagcagagct tggtcttcga tggtctcttg cgcgaggttg ccttgcgcca
     4081 acgcacggtc caaggcggca tcgacaaccg ccggaccaac cgagagcagc ttctccgcct
     4141 cctccttcag cgcctcgttc ggcagtgcac agtggccgtc gttcgtcgct gtgagcagca
     4201 catgatccag accggcgcag gcccgctcga tcgagtcgtg cggcacgcca agtttccggg
     4261 cgatttcatc tgctgtcttg aatccgatcc catggatgtc acgggccagg cagtacggat
     4321 cgcgccgaac ggtttcgacg gcctgagcgc cataggtcct gtaaatgcgg acggcgcgac
     4381 tggtgctgac cccgtgagag tggaggaaca ccatgatctc gcgcaccgcc ttctgttcgg
     4441 accaggcctc cttgatccga tggcgtctct tgggaccgat cccctcgacc tcctcgagtc
     4501 gcgccgagaa acgatcgatg atgtcgaaga tttgctcccc gaacttcgcg accagcttcc
     4561 gtgcgtacac gggaccgatc cctttgacca tgccgctgcc caggtacttc tcgatcccct
     4621 cgcgcgtcgt cggagccgtg ctggtcagca catcggcccg gaattgaagc ccatactcgc
     4681 ggtcctgcac ccaacgccct tccccgtgga tgtattcgcc tgcggcaacc gatgcgagcg
     4741 cgccaaccac acagaccagg tcgcggtgcc cacgcgcctt gaccttcagc acggcgaacc
     4801 cggtgtcttc gttgaaaaac gtcacccgct cgatcaagcc ggcgaggctt tccggaggcg
     4861 tggcgggcga ggcggactgc acggatgccg atcatgcgca accggcccac ggctggcaag
     4921 cagggcggat tcagtattca gtaaccagtg cagtgacacc tgcctgccca cgctcttttc
     4981 atatgcaagg gtgcttgagt agtggttgca gggggatgtg tggggtgagt gcgtccggca
     5041 caccctgaac cgccagtcgc tgattccgcc ggatggccgc gttcacagcg tcgagcccgg
     5101 ccttgcctgc cgcgacgggc actgcgcctt gcccttcggc gggcgcgcgc ctagaattgg
     5161 ggcgtgtcag atcagaccga tgccataacc agtgcaatgc ctgttgtgcg agaccacgcg
     5221 cgggtgttcg atcgtttcct ctttgtttac cccgtcatct cacggcgttc gggcggcctc
     5281 tcgctgggag tcaatctcaa cccggacaaa cgctgcaact tcaactgcgc ctactgtgag
     5341 gtcgatcgcc aaacacccgg accagccaaa acagtcgatc tcatccgctt gcgcgccgag
     5401 ctgagcgcat tggcccaatc agctctccgc ggacagttgg gtctgacgcc caagttcgcc
     5461 gaggtcgcgg ccctcacccg ccgcatccgg gacattgcct tcagcgggga tggcgagccg
     5521 actatggtcc ccaatttcgc cgactgcgtg cgtgcggtga tcgaggtgaa gctggccctg
     5581 gcgttggccg acaccaagat cgtgctgatc accaacgcga cgggcttgga taagaccgac
     5641 gtcaagcgcg gcttggcgct gctggacgcc catcaggggg aaatttgggg caaactcgat
     5701 gccggcaccg atgcctacta ccggttggtc aacagaagtc atgttgcctt cgaccgtatc
     5761 ctgcacaacc tgaccatcac agcgcaggcg cgcccaatcg tgatccagag cctctttatg
     5821 aaagtgcgcg gcgaaccgat gcccgcccct gaactggaag tctattgcgg gcggctccgc
     5881 gacatccttg cggcgggcgg atccattcgc gaggtgcagg cctacaccat cgcccgcccc
     5941 acaccggagc cgtgggtcga gcgcctcgat cgcgcggatc tcgactccat cgccgcggtg
     6001 atccgcgccc gcaccgggct gcccgtcatg accttcgcgt gatggtccat tcccccgcca
     6061 tactctgatg aatgccgccg tcatcgtcgc cgcgggacgc ggaacccgga tgggaccgga
     6121 ggtggacaaa ctctttctcg aagcggcggg gcgcccgatc atcgcgcaca cctggcagcg
     6181 attccaggat gccgactgca ttcacgagat cgtgcttgtg gtgcgcgcgg gcctcgagaa
     6241 ggcctttccc gagatggcga ctcgcttcgg cttccagaaa ccgtggcgcg tcgtgacagg
     6301 cggcgcggag cgtcaggatt ccgtctggaa cggccttcag gccgtgtccg atcaaacgca
     6361 agtggtggcc atccaggacg gggcccggcc ctgcacgacc gccgcgttga tccgcgcgac
     6421 catcgacgcg gcgcaggtaa cgggcgcggc cgtcgccgcg cagcgggtga ccgacacgat
     6481 caaggaatcc gacgacggcg tgatgattgt ccgccatccg gaacggtcga ggttttgggc
     6541 ggtgcagacg ccgcaggcat tccgaatcgg tgtgatccga cgcgcgctgg cggctgtgcg
     6601 tgaacggggg gcgcgggtga ccgacgacac agccgcctgc gatctgatcg ggcagcgggt
     6661 tcaattggtc gaaagcaggt ccccgaaccc caagatcacg gtgcccgacg acctcgcgct
     6721 gctggaatgg cgcctgagcc ggatgaacga cccgtcccgg acagcggccg atcgaggcgg
     6781 ctcgcatgct ttgcgggaaa tcgcgacgat ctaaaccggg ccgcgactcg acgcgatctt
     6841 cctggctgat cccccacgct tgctaacggc tcacacgacc gttactttcc ggcacatggc
     6901 ttttctttga agggactatg acaaagagac tgatgtattc ggtgttgttg acggctctgg
     6961 cggtcaacct gttcttcggc gcccaggtct atttgcgttc ggccgaggtc gccgaaaaga
     7021 acgacgtcta cgggaacctg cgcctgttct cgctcgtcct cgagagagtt cgcaacgatt
     7081 acgtggatgg cgagaacctg acgtatcagg acctgatcta tggcgcgctc aaagggatgc
     7141 tcagcacact cgacccccac agcgagttca tggagcccca gaagtacgac gagctcaaga
     7201 aggacaccga aggcaaattc ggcggcgttg gaatccaggt tgggctgcgg ggcgagtacc
     7261 tgaccgtcat cgccccgatg gacgacaccc cggcctcgcg ggccggaatc atgtcaggcg
     7321 accgaattct gaaaatcgac ggccgaagca cggagaagat cagcctcacc gaggccgtca
     7381 atagaatgcg cggcgcgccc ggcaccgagg tcgtcctgac cgtgctccgc ccgtccacgg
     7441 gcgccacgaa ggatcacaag ctcgtccgcg cggacatcgc ggtcgagacg gtcaaggaca
     7501 ttcacgggaa gagggagttt tctgtcgacg acgacaagct cggctacgtg cgattgacgc
     7561 aattcggaga agcgacaggg gaggaactgg accgcgccct caaaaaactg catgaccaga
     7621 aaatgcgcgg tctggtcctg gatctgagaa acaaccccgg cggcctcctc gaccaggccg
     7681 cgcacgtctg cgaacggttc gttcaaccgc gccaactggt cgtcaccacc gagggtcgcg
     7741 atccctccga acggcgcgag taccgcgccg gccgccgggg agaacggacc accgtgccga
     7801 tggtcgtttt ggtcaacggc ggcagcgcga gcgcatcgga aattgtcgcc ggctgcctcc
     7861 aggacctcaa gcgcgcggtt gtcctgggcg aaaagacatt cggcaaaggc tccgtccaga
     7921 gcatcattcc gctccaggac ggagccgcgt tgcggctgac gacggccaag tactacacgc
     7981 ccagccacaa ggtgattcat gaaaaaggga tcgagcccga catcgtggtg ccggtgccga
     8041 tcgaggatgc cgatgcgctc ttactccagc gcacgcccgg cctgatcgaa tcgctcgatg
     8101 cggaacgtca ggaacggttc ggcgccgtgc gggatatcca actcgaacgc gccctggatc
     8161 tcctcaaggg aatcaccctc tatacccatc gagcgcccga ctccgaccgg aggcccgaca
     8221 ccaagccgat ggccgccaag gcggactgac cggtgaccct gcttgccatc gagacctcct
     8281 gcgatgaaac cggggtcgcc gtggtgcgca acggccacgt tctggcgaac ctggtttcct
     8341 cacagaccag cctgcacgcg gagtacggcg gtgtcgtccc cgagttggcg actcgtgagc
     8401 acttgcgcaa cctccccgtg ctgatccagg ccgccttgtg cgaggcaggg accgacccgc
     8461 ccgcgatcga agccctcgcg gcgacgcagg gtcccggctt gcccaacgcg ctcatggtag
     8521 gattgaaagc cgcccaggga atcgcgtgcg cgctgcggcg gcctttcctg ggcattcacc
     8581 accacgaagc ccatctctat tccgcatggg tcaaagggga cccgccgcac gccgacttcc
     8641 ccgccatcca accgcacgtg gcgttgatcg taagcggcgg ccacacgctc ctcatccatg
     8701 tccccgaagg cttgcgccat cgcgtcctgg gctcgaccct cgacgacgcc gcgggggaat
     8761 gcttcgacaa gaccgcgaaa ctgctcggcc tgccctaccc gggcggaccg gtcatggacc
     8821 agctcgccgc tgaaggcaac ccggctgcgt ttcctttccc gcgtccgctg ctgaacgatg
     8881 cggcatacga tttcagtttc agcggcctca agacctccgt gcgctatttc ctgcgagacc
     8941 acccgggttt ggcggacgac ccacaagcgc tgcgcgacct ctgcgcgagc gtgcaggcgg
     9001 cgatcgtcga ggtgctcctg gccaagacca cccgcgccgc ccgcgacctc ggcgttcgtc
     9061 tcgtcaccgc ctcgggcggc gtcacctgca atcgggcttt gcgccgcgat ctggcgtccg
     9121 cgtgcgcccg cgatggcctc gacttgcgcc tggcgccgac ggccttctgc acggacaacg
     9181 cggctatggt cgggctcctg gccgagtgga agctggcgtc gggcgttccg cctgatgatc
     9241 ccgacggcga tgtgcgcccc gcatggtcgc tcgaatcgat gccggcccga ggcgggtgaa
     9301 atcgaacttc gatctcatct cgcgcaccaa acgaccctcg atccgcttca tcctccgtcg
     9361 ccagcctggc gtaacgtcgt cgtagccgcg gaggtggagc aatccgtgga gcaggtagcg
     9421 caccagctcg gatgtccagg tggtccgata ccggcgcgcc tgaatgccag cctcatccag
     9481 acacacaaaa acctcgccgc acagccaggg atcgccgggc cgggcgcccg gtggcgggcc
     9541 atagtcgaat gcaatcacat ccgtcacacc cgcgtgggcc agatagcgcc cattgagcgc
     9601 cgtaatctcg tcggcgccca ccaggaaaca gcccagttcg tagcaaatcc gggcgcgggg
     9661 gctgtcggat gcgccagacc gcggcgtccg aacttgaaac gcgcggcgcc cgcgccgagg
     9721 cgcggcttcg ggggctggcc gcgagaacac agggtcggcg agagccgcgc gcaagatgcg
     9781 gcgaagtaac ctgcggtcga cccggattcg gggatgccgc tgaacgagcg tcagttcacg
     9841 ccggctgcga agcgcgatgc tgttcgtagg catgcacgat gcgggcgacc agaggatggc
     9901 ggatgacgtc cttcttgccc aactcgatga cggcgatccc ctcgacctgg gcgagggcct
     9961 ggcgggcttc gatcaagccg gacggcttgt gcctggggag gtcgatttgg gtgatatcgc
    10021 cggtaatgac cgccttcgaa ttgtggccca gacgagtcag aaacatgaac atctgctcgg
    10081 tcgtcgagtt ctgcgcctcg tcgaatatga tgaaggaatt gttcagcgtg cgcccgcgca
    10141 tgtaggccag cggcgcgatc tcgatcgtgc cccgctccat caaacgctga atctcctccg
    10201 cgggcagcat gtcgtgcagc gcgtcataca aagggcgaag gtacggcgcc agtttctcct
    10261 gcaaatcccc cggcagaaaa cccagcgcct cccccgcctc gaccgccgga cgggtcagaa
    10321 tcagccggtt caccttcgca ccgcgtagcg ccgccgtggc catggccatc gcgaggtatg
    10381 tcttacccgt gccggccggg cccacaccca gagtgatatc gtgctgccga atcgcctcga
    10441 tgtaacgccg ttgccccgtg gtcttcgggg tgacagtggc cttcttcggc gaggtttgga
    10501 tgcgctcggc ctggaggctc tcgagagccg caacgccctc gttccgcacc acgctcaggg
    10561 ccatcgcgaa gtcctgggtc cgcacatgcc cgcccgtctt gagcgtccct tcgagcaact
    10621 gaaataaatg ccgagcccgc gtcacggcct cggtttcccc ctcgagtttg atccaggcgt
    10681 cccgagcggt ggccttcacc cccagttgcc gttctaacgc cgacaggttc cgcgcgtcgt
    10741 tgttgaacaa ctgctgtgca agacgggcgt tgtcgaaatg aagtgtttca gtaggcattc
    10801 gaaaagtgcg cgctcaagac cgattcccga tctctccccc gcccgcccca atgacaggcg
    10861 cgttcggggc atgggacgtt cggacaccct cctccttgtc catggccagg gcagcgcgaa
    10921 gcctggcagc cacatcgtca agcgcctccg gcatgacggt cgtctgggcc accacaggca
    10981 tgaaattggt gtccccgttc caccgcggta cgatgtggat gtggaggtgg tcagccacgc
    11041 ccgcccccgc gacccgcccc aggttgattc ccacgttgaa tccctccggc ttcatcgccc
    11101 gggtgagtgc attcagacac cgacgagtca gaaccatcag atcggtgagc tcctgctcgg
    11161 tcagatcgtt cagatccgcc gtccgccggt acgggacgac catcagatgc cccccggtgt
    11221 aggggtaggt gttgagcacg gcaaaacagc tcttctcccg cacgagcaca tggttggcta
    11281 cgtcgtccgc cgcctgcgcg atgtgcgtga acagcgattc atcgctgggc ggcggcttgg
    11341 gcgcgcgtat gtactcgata cgccatgggg catggaggtg ttccatgttc agacgctagc
    11401 gattgaggcc atgcctgtca aagccagagg tgttgacgct cgaacacgac cccgcccact
    11461 ccggtccgat ggcgtcccga gggcttgaat cgccgccgcc ctttgcgcta ggcttacgcc
    11521 atgaaatcag ttcgactctt cctcacactg tgcgcctcgc tctgcctcct ccgagcccag
    11581 gacgacaacc ttccaaccct ttttgtcgcc ccgttcgacg gtgacaggtc tgagatttcc
    11641 tattggcagc ccgccttggg cgaaggcctc gccgagatgt tgatcaccga actcagcaag
    11701 acaggaaagt tccaaatgct ggaatcgacc gcaatcgagt ctctcaaaga tgaaatcagg
    11761 atgggcgagg atggttgggt ggccaaggat gagcgtgtgg aaaagggggg atgggccggc
    11821 gcggactaca tgttccgcgg caaagtcacc cggttcggc
//
LOCUS       DAOQRO010000062        11781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4596_length_11781_cov_4.539485, whole genome shotgun sequence.
ACCESSION   DAOQRO010000062 DAOQRO010000000
VERSION     DAOQRO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4596_length_11781_cov_4.539485"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PKK20_03755"
     CDS             <1..1543
                     /locus_tag="PKK20_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNU99033.1"
                     /translation="SFDPSLPASATAFRTRYNLDAATRLFGPYRGQLANDGDSVELLQ
                     ADGQRESDTEYAARFVLADRVAYRDTSPWPEAADGLAFSLQRLVETEFGNDPANWTAA
                     APSPGEAYVAAAPPVITRQPLSQTAAAGSQITLRVEASGSGPLSYQWRLNGKNLAGQA
                     DSLTLTNLHASHAGEYRVVVSSAVAAVVSQPATIRVATPPTLLASPQSIEVLAGQPIE
                     LSVVAVSSSPLRYQWRRDGALVDGATESTLSFASAEARDTGDYEVIVQNDTDTAVSTV
                     ASVRVVGHPVIVRQPWSATVIEGGTVTLGIEVNPQASLPIRYRWIRNGAAVFERALAS
                     HVDFLTLTNVRAADAGTYWVSVKNPGALASGVTSEEATLRVVIAADADRDGLPDAYEI
                     RCNLNPNDPTDAAADTDGDGASNREEYLAGTDPLDPDSVLRILSIDASDGVSVTFQGV
                     ANRNYTVQYRDRTTPGSWHTLALIPAVSGLSTAERLIELRDPLGTSPNQRLYRIGTPG
                     LSEPR"
     gene            complement(1540..4185)
                     /locus_tag="PKK20_03760"
     CDS             complement(1540..4185)
                     /locus_tag="PKK20_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNU99034.1"
                     /translation="MASRLYRGVAGWHKNGTNMRTRTSILAFFASVVCLPLLLGVQDG
                     PSPSRIDLPGRRADGSVLLPNQWSLRPVGRQVTLGDFPVNIAVHPGGSHAAVLHSGYG
                     DHEIAIVDLGQGKIVTRIGVTEAFYGLAFSSDGARLYCSGASDEVIHAFRFEGGYLGE
                     YRPLRVRPVADRGVPGGLAVSRDGSQLWLANVWGHRLTGLDPLTGDRIVDFALGEQLA
                     AGLDLSLPPADPDIAAATKRDEAQSDPTKPASPYPYGCVLDEPRQRLYVSLWAKACVA
                     VFDLRTRRVSALWQVQEHPNEMALAPSGAVLFVANANRNTVSVLEAETGRTLETLNSS
                     LYPQSPPGSTPNSLALTPDGALLFVANACNNNVAVFDVSEPGRSRSLGFIPVGWYPTS
                     VRVTPDGRRLLVANGKGIISRANRHGPQPGELDPPRTVREYIGGLFRGTLSLIDLPES
                     RGKLVERLKNWTAQAYRCSPLEPDARVKARPAQGHPIPSRVGDPSPIKYCIYILKENR
                     TYDQVLGDLPEGNGDRSLCLFPESVTPNHHQLAREFVLLDNFYVDSEVSADGHEWSMG
                     AYATDYVEKTWPMSYGHNQRGKYSYPSEGAFRIAAPSCGYLWDRAKEAGISYRSYGEF
                     VVNGRTPSDPARTLIPALVGHIDPGFRGWDMDYLDQGRADRFISELQRFEREGNMPRL
                     QIMRLPNDHTYGTSAGKRTPRAMMADNDLALGRVVEAVSQSKFWPRTAIFVVEDDAQN
                     GPDHVDAHRTVAYAISPYARRGAVDSTMYSTCSMLRTMELILGLQPMTQFDAAATPMY
                     NSFQDRPDTRPYRHRPVTVDLEERNTPLAWGADESARMDFTKEDAADDIRLNEVIWRS
                     IRGPDLPMPAPVRAAFVFAGQDDDD"
     gene            4329..4658
                     /gene="trxA"
                     /locus_tag="PKK20_03765"
     CDS             4329..4658
                     /gene="trxA"
                     /locus_tag="PKK20_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013662070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNU99035.1"
                     /translation="MTNVTEISSAQFPSEVLKSTVPVMVDFYAPWCGPCKMIAPLLDT
                     VAGEFAGRVKVVKVNVDDASDLANQFHITSVPTLVLFKNGQVSDTMVGAPSPAALRSK
                     FESAIAG"
     gene            4658..5473
                     /locus_tag="PKK20_03770"
     CDS             4658..5473
                     /locus_tag="PKK20_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99036.1"
                     /translation="MSKARKDASRRGPPRKPSGKVGLAVAGVFILLLAGLAWHGAARR
                     SPGPSGGAHPNQSRAQDPASGSGANSTESGPDGGAGAEAASLVTRGNELLRQGQFAEA
                     AKVYEEAKRLTPEDEDVHYNLGIAFARMGRQEEAVASYRRALELFPQYAEAHNNLGIL
                     LHRMGKLDEAFEHFRAAIKAVPDYASAYNNLGNALRQRGLVAEATDAFLKASQLDTNY
                     WQARFNLANSYAALDRLDDAIAEYEAVLRIEPDFAPAQQALEQTRLKRDSHSP"
     gene            complement(5498..6178)
                     /locus_tag="PKK20_03775"
     CDS             complement(5498..6178)
                     /locus_tag="PKK20_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNU99037.1"
                     /translation="MLEIRPSVIETRPAMPGDPRALVARLGRPGLRAVRAVLAGLARA
                     QVKDTASLRSFLIEYQTQVLGPVELTAIQRARWHADRNEVRELLRLDRQLGEDARLSR
                     FAAASRAVGRAQLRRLQPLKDVRLVRRYWEAVGAGRASAWHPLVYGVMLSVYSIPMRQ
                     GLLHYGGRTLGGFLDAAATPLRLSLEDCCSLAAELDRALPALVERSLLPCEPSRDCVC
                     ARLPAPGA"
     gene            6431..10252
                     /gene="smc"
                     /locus_tag="PKK20_03780"
     CDS             6431..10252
                     /gene="smc"
                     /locus_tag="PKK20_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNU99038.1"
                     /translation="MYLKTLSAFGFKSFAEKTTLVFQPGLTAIVGPNGCGKSNVADAI
                     RWVLGEQSVKALRGGEMTDVIFNGTDNRKPLGLAEVSLTLGGVDKDHLKAAGVELAYD
                     EVTVTRRVFRNAGSEYFINKTPCRLKDIQQLFAGTGIGRASYSIMAQGNITQILSSRP
                     EDRRLVFEEAAGITRFKAQKREAIRKLEGTEQNLLRVADLIREVKRQIISLQRQAGKA
                     RRYKQYMAELQHLDTQLARHQYDVSQLDIEEGRERIEALRGEIETCSGEVVRGEEEVS
                     QLRERLAELEHAISSSQQRGLELKAEAERHEHRIQYNEERLRELAARNEKALADIAEA
                     EERKIAAERALAETAERLESAEAKLGDARRQLEARREGLKELEDDARLKQEAMRQAQS
                     DVFDVAQQLTRARNEINALDLQKQGNVIRIEKLSAEKIQLEEERAGLAKRLEEFARES
                     EAEQVALQTRRSSFQERQERLRTVQREIGQAGLDLDQLQRRQAETHSRLNVLQQLESS
                     REGFGAGALAALKDTQQVLGSLADRIRVPDEYVPAIEAALGSQLQIVLTHSPDVARQI
                     LADLSAHQKGRASIASLELATARREASRDATSPPIAAETAPEAAACPRAEDPLEARGC
                     VAALGVVEGDAAVLPLLESLLGRTRIAPDLEAATTAWRACGGRFDFVTRAGDLLNRHG
                     VFTGGSSNGSSKGPSSILGRKNQIAELERSLAEVHARVNDASRRKGTLLSEQTALQAS
                     LQQAQSELQAQEVAIATRQGEFKALQNAQRMLEQKVDTVVFEIQTLAGQEREGSERRA
                     SLSAQVGELEARDHQAQRRLTELTGALEQLRVRRDQLNAAVTEAKVAVATEEQLAASF
                     RQQQGPLEQRIRELAHLLEQRRQEIAQVLDRRGQAQMEIEDSRGRIGAVQHERDQLGL
                     RTADLLAQRDAQADDIATREEALRNSRRRLTEQQELRSAAEVDLAQKEMGLRNIVERI
                     QERYQINLADVRSECITITYAAEGPARVETLTPEQMAAAGVSTDWNAVAEQVAALQAK
                     IDEMGPVNLVAIEEYEETEQRHQFLSSQHDDLIKAKEQLVEVINRVNTQTRQMFTETF
                     HNIRDNFRSIFIEIFGGGAADLNLVDEGDVLESGIEIMARPPGKQLRSISLLSGGEQT
                     MTAVALLFAIYQVRPSPFCVLDELDAPLDESNIDRFIRVLQRFLPHSQFIVITHNKRT
                     IGMADVLYGITMQEQGVSKIVSVRFHKTDTPIADRAAASIETSIDGPSIGVEEDHEIG
                     LEETLEVTIAE"
     gene            10395..>11781
                     /locus_tag="PKK20_03785"
     CDS             10395..>11781
                     /locus_tag="PKK20_03785"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNU99039.1"
                     /translation="MQNRSRLLLRVAMLCLLLSGMAGLVYEIVWTRYLALFLGHTSYA
                     VIAVLVAFMGGLALGNLWIGVWADRTRRPLASYAWLEIAIGVYALIFPIHYQLCYSGF
                     LGLARSLDPGSAGLLALKFLFSLLAILIPTVLMGGTLPLLTRLLTRSLGEMREKVAAL
                     YFINSAGGVIGIWLADFWWIGAIGLPATLMVGAVLNLAVGGLALFVSGYIKEGQADQD
                     EATTRLADTPRPEAEPESYGPVELRLAILGIGLSGFVAMLYQVAWTRLLALAMGSSTH
                     AFSLMLITFITGLTVGAWIVYRWRSLRRSLAAFAWAELALALTVGVSLFFYEFLPYWF
                     GRLADLLARRPEAYSLYQGLQGLLCFAVMFVPTVCLGMTLPLVTRAATPSLERAGRTV
                     GRVFAVNTVGTVLGAALTGLVLMPRLGLAGTFVLGVAVNLLIGLVIVVRVERGAKRLI
                     AIGASGLFVVFL"
BASE COUNT         2191 a         3957 c         3680 g         1953 t
ORIGIN      
        1 cagtttcgat ccgtctcttc cggcttctgc caccgccttt cggacccgct acaacctcga
       61 tgccgccaca cgcctctttg ggccataccg gggccaattg gcaaacgacg gcgacagcgt
      121 cgaattgctg caagccgacg gccagcgcga atccgacacc gagtatgcgg cgagattcgt
      181 cctggcggat cgggtcgcct accgcgatac atcgccatgg cccgaggcgg ccgatggact
      241 cgccttctcg ctccaacgac tcgtcgagac cgaattcgga aacgatccgg ccaattggac
      301 ggccgcggcc ccatcgcccg gcgaggctta cgtggccgcc gcgccacccg tgatcacccg
      361 ccaaccgctg agccaaaccg ccgccgccgg atctcagatc actcttcgtg tcgaggcaag
      421 cggctccggt cccctgtcgt accagtggcg attgaatggc aagaacctcg ccggccaggc
      481 cgactccctg accctgacca atctccatgc ctcgcacgcc ggcgagtatc gggtcgtggt
      541 gtcgagtgcc gtggccgccg tcgtcagtca gcccgccaca atccgcgtgg cgacgccgcc
      601 gaccctgctt gcctcgcccc aatcgatcga ggtcctcgcc ggacagccca tcgagttgtc
      661 cgtcgtcgca gtcagttcct cgcccttgcg ctaccagtgg cggcgcgacg gcgctctcgt
      721 cgacggcgcc acggagtcca cgctttcctt cgcctccgcc gaggcccgcg acacgggaga
      781 ttacgaagtc attgttcaga atgacacgga caccgccgtc agcacggtcg ccagtgtgcg
      841 cgtcgtcggc catcccgtca tcgtccgcca accgtggagc gccactgtca tcgagggagg
      901 gaccgtgacg ctgggcatcg aggtcaatcc ccaggcgtca ttgcccatcc gttaccgctg
      961 gattcggaac ggcgccgccg tcttcgaacg cgcactcgcg tcccacgtcg acttcctgac
     1021 cttgaccaat gtgcgggccg ccgatgccgg cacgtattgg gtgagcgtca agaatcccgg
     1081 cgccctcgcc tcaggcgtca ccagcgaaga ggcgacgctc cgggttgtca tcgccgccga
     1141 cgccgaccgc gacggtctcc ccgacgctta cgaaatccgc tgcaacctca atccgaacga
     1201 cccgacggac gccgccgctg acaccgacgg ggacggcgca tccaaccgcg aagaatacct
     1261 ggcgggcacc gacccgctcg atcccgacag cgtgctcagg attttgagca tcgacgcaag
     1321 cgacggcgtg tccgtgacgt tccagggtgt ggccaatcgc aattacaccg tgcaatatcg
     1381 cgatcgcacc acgcccggtt cctggcatac actcgccctg attcccgccg tttcgggtct
     1441 ctcgactgcc gagcggttga tcgaattgcg cgatccactc ggcacatcgc caaaccagcg
     1501 cctgtaccgc atcggaaccc cgggtctgtc cgagcctcgc taatcgtcgt cgtcctgccc
     1561 ggcaaaaacg aacgcagccc gcacgggcgc gggcatcggt aggtcgggtc cgcgaatcga
     1621 acgccagatg acctcgttga gccggatgtc atcggccgca tcttccttgg tgaaatccat
     1681 gcgcgcggat tcatcggcgc cccaggccag cggcgtgttg cgctcctcga gatctacggt
     1741 caccggccga tggcggtaag ggcgcgtgtc gggtctgtcc tggaatgaat tgtacatcgg
     1801 cgtcgcggcg gcatcgaact gggtcatggg ctgcaatccc aggatcaatt ccattgtccg
     1861 caacatgctg cacgtcgagt acatggtcga gtcgaccgct ccccgccgcg cataggggct
     1921 gatcgcatac gccacggtgc gatgggcatc gacatggtcc ggtccgttct gcgcatcgtc
     1981 ctcgacgaca aagatcgcgg tgcggggcca gaatttcgac tggctcaccg cctcgaccac
     2041 gcgccccaga gccagatcgt tgtcggccat catcgctcga ggagtgcgtt tcccggccga
     2101 tgtcccatag gtgtggtcgt tgggcaagcg catgatctgc aggcgcggca tgttgccctc
     2161 gcgctcgaac cgttgcagtt cgctgatgaa gcggtcggcg cgcccttgat ccaggtaatc
     2221 catgtcccat ccacggaatc cggggtcgat gtgaccgacg agggccggga tgagcgttcg
     2281 ggcgggatcg cttggtgtgc gcccgttcac gacgaactcc ccgtacgacc ggtagctgat
     2341 gccggcttcc ttcgcccgat cccagaggta gccgcacgaa ggcgcggcga tgcggaacgc
     2401 gccctcgctc gggtaagagt acttgccgcg ctggttgtgg ccgtaggaca tgggccaggt
     2461 cttctcgacg taatcggtcg cgtaggcccc catgctccat tcatgaccgt cggcgctgac
     2521 ctcgctgtcg acgtagaagt tgtcgaggag gacgaattcc cgggcaagct gatggtggtt
     2581 gggggtcacg ctctccggaa acagacacag actgcgatcg ccgttgcctt cgggcaaatc
     2641 gccaaggacc tggtcgtagg tccgattctc cttgagaatg taaatgcagt acttgatcgg
     2701 actgggatcg ccgacccggc tcgggatggg atgcccctgc gcgggccgcg ccttgacccg
     2761 ggcatcgggc tcgagcggac tgcaccgata ggcctgagcc gtccagttct tgagccgctc
     2821 gaccagcttg ccgcggcttt ccggcagatc gatcaggctg agcgtgcccc gaaacaggcc
     2881 gccaatgtac tcgcggaccg tgcgcggcgg atcgagttcg cccggctggg ggccatgacg
     2941 attggcgcgc gagatgattc ctttgccgtt ggcgaccagc agccggcgtc cgtcgggcgt
     3001 gactcgcacg gatgtgggat accagcctac gggaatgaaa ccgagtgatc ggctccgacc
     3061 cggctcgctg acgtcgaaca ccgccacgtt gttgttgcag gcgttggcca caaagagcag
     3121 cgccccgtcg ggcgtcagcg ccagactgtt gggcgtcgat ccgggaggcg actgaggata
     3181 cagcgacgag ttcagtgtct cgagagtccg ccccgtctcc gcttcgagaa cgctcacggt
     3241 gttccgattc gcgttcgcga caaacagcac ggctcccgac ggcgccaaag ccatctcgtt
     3301 tggatgttcc tgcacctgcc acagggcgct cacccgccgc gtcctgagat cgaacaccgc
     3361 aacgcacgcc ttcgcccaca agctgacgta aagtcgctgc cgcggctcgt ccaaaacgca
     3421 gccgtaggga tacggcgagg ccggcttcgt cggatcggac tgcgcttcgt cgcgcttggt
     3481 cgcggcggcg atatccgggt cggcgggcgg caatgacaaa tccaggccgg cagccaactg
     3541 ctcgcccagg gcgaaatcga caatccggtc ccccgtgaga gggtccagac ccgtcaaacg
     3601 atgaccccaa acattggcca gccagagttg cgagccatcc cgactgacag ccaggccgcc
     3661 cggcacgccc cgatcggcca cgggccggac gcgcaacggg cggtattcgc cgaggtaacc
     3721 gccctcgaac ctaaaggcgt gaatgacttc gtcactcgcg ccgctgcagt acaggcgggc
     3781 gccgtccgac gagaacgcga gcccgtaaaa cgcctcggtc acaccgatgc gcgtcacgat
     3841 ttttccctgg cccagatcga caatcgcgat ttcatgatcg ccgtagccgc tgtgaagaac
     3901 cgcggcgtgg ctcccgccgg ggtggacggc gatgttcacc gggaaatcgc ccaacgtcac
     3961 ctgccgcccc accgggcgca gcgaccactg attgggcagc agcaccgaac cgtcggcgcg
     4021 ccgccccggc aagtcgatcc ggctcggaga gggtccatcc tgaactccaa gcagcaacgg
     4081 caagcacacc acggatgcaa aaaaggcgag gattgaggtt cgagtccgca tattggtgcc
     4141 gttcttatgc cagcccgcaa cgccacggta aagcctcgat gccatgtcgc cattgtcttt
     4201 tgcgcgcgct gggtccacac tccacccgca tgtaaccttt tcgaggctgg cggctcttcg
     4261 gaaagcggca agtgacaaat cgagactgaa gccgaatctg aatctgaatc tgaacctgga
     4321 actgatctat gaccaacgtg accgaaatct cctccgccca attcccgagc gaagtcctca
     4381 agtccaccgt ccccgtcatg gttgatttct atgcgccgtg gtgcggcccc tgcaagatga
     4441 tcgcgcctct cctggacact gtggccggcg agttcgccgg gcgcgtcaag gtggtcaagg
     4501 tgaacgtgga cgatgcgagc gatctcgcca accaattcca cattaccagc gtccccaccc
     4561 tggtgctctt caagaacggc caagtctccg acaccatggt cggcgccccg tcgcccgccg
     4621 ctcttcgaag caagttcgaa agcgcaatcg ccggttgatg agcaaggcgc gcaaagacgc
     4681 gagccgcagg ggcccgccac gaaagccgtc tggcaaagtc gggctggcgg tggcgggcgt
     4741 cttcattctg ctcttggccg ggctggcctg gcatggcgcg gcccggcgct ctccaggccc
     4801 ctccggcggc gcccacccaa accagtcccg cgcccaagac cctgcatccg gctcaggggc
     4861 caactcgaca gagtcggggc cagacggcgg cgcaggggcg gaagcagcca gcctcgtcac
     4921 ccgtggcaat gagttgcttc gccaaggcca attcgccgag gccgccaagg tctacgagga
     4981 ggccaaacgc ctgacgcccg aggatgagga tgtccactac aatctgggga ttgcattcgc
     5041 ccgcatgggc cgccaggaag aggccgtcgc atcctaccgc cgcgccctgg aactgttccc
     5101 ccaatatgcc gaggcgcaca acaacctcgg catcctcctg catcggatgg gcaagctcga
     5161 cgaggccttc gagcatttcc gcgccgcgat caaggccgtg cccgattacg cctccgccta
     5221 taacaacctc ggcaacgcgc tccgccagcg gggtctcgtg gccgaggcga ccgacgcatt
     5281 tctcaaggcc tcgcaactcg acaccaacta ctggcaggcc cgcttcaatc tagcgaactc
     5341 ctacgccgct ctcgatcggc tcgatgacgc cattgccgaa tacgaagccg tcttgcggat
     5401 cgagcccgat ttcgcccccg ctcagcaggc gctcgaacaa acgcgcctga agagggattc
     5461 gcactctccc tgatcgcaat gcggaccgtg ccccggctca cgcgccagga gccggcagcc
     5521 gtgcgcaaac gcagtcgcgc gaaggctcgc acggcagcaa actccgctcg acaagcgcag
     5581 gcagcgcgcg atcgagttcc gccgcgagcg agcaacaatc ctccagcgaa agcctcaagg
     5641 gcgtcgccgc cgcatccaga aacccgccca gggtccggcc cccgtagtgc agcaacccct
     5701 gacgcatggg gatcgaatag acggaaagca tgacaccgta aaccaacgga tgccaggccg
     5761 aggcccgccc cgccccgacc gcctcccagt accggcggac aaggcgcacg tccttgagag
     5821 gctgcaaccg ccgcaattgc gcgcgaccca cagcccggct cgctgcggca aaacggctca
     5881 gccgggcgtc ctcgcccaat tgccgatcca gccgcagaag ttcgcgaacc tcgttccggt
     5941 ctgcatgcca acgcgcccgt tggatcgctg tcaattcaac cggaccgaga acttgggtct
     6001 ggtactcgat cagaaacgac cgcagcgacg ccgtgtcctt gacctgagcc cgagcgagtc
     6061 ccgcgagaac tgcgcgcacg gcgcgaagcc cgggccgacc aagccgcgcc accaatgccc
     6121 gcgggtcgcc cggcatggcc gggcgagtct cgatcacgga tggtcgaatc tcgagcacgc
     6181 gcttcgcttc aatctcccgg cttggcagcc tcgtgctcca gaccggcgac aaagtaacca
     6241 gaccctactg acacccacgc tgccgtgcca actggaaaag aacaataccg gcccgaatcc
     6301 caatacaaaa ctggcaaccg ggatagaaac cgggcatccc tattagttgt gcggcagaca
     6361 agactctccc acattatctt gtggttttca gctttacacg ctgggtcatt ttccggtatt
     6421 ccgtccagcg atgtacctga agacactgtc tgccttcggt tttaagtcgt tcgccgagaa
     6481 gacgaccttg gtttttcagc ccggactcac cgcgattgtg ggtccaaacg gctgcggcaa
     6541 gtcgaatgtt gcggatgcga ttcgctgggt attgggcgag caatcggtca aggccctgcg
     6601 cgggggggag atgaccgatg tgatcttcaa cggcacggac aaccgcaaac cgctcgggtt
     6661 ggccgaggtg tcgctgacgc tcgggggggt ggacaaggat cacttaaagg cggcaggcgt
     6721 ggaactggcg tacgacgaag tgacagtgac tcgccgggta tttcgcaatg cgggaagcga
     6781 gtacttcata aacaagacac catgccggct caaggacatc cagcaactgt tcgccggcac
     6841 gggcatcggg cgcgccagct acagcatcat ggcgcagggg aacatcacgc agatactctc
     6901 gagccggccc gaggatcgcc gcctggtctt cgaggaggcg gcaggcatca cgcgtttcaa
     6961 ggcccagaag cgggaggcga tccgcaagct cgaggggacg gagcagaatc tgctgcgcgt
     7021 ggcggatttg atccgcgagg tgaagcgaca gatcatctcg ctccagcgcc aggccggcaa
     7081 ggcgcggcgc tacaaacagt acatggccga gttgcagcat ctcgacaccc aactggcgcg
     7141 gcatcaatac gacgtgtcgc aactcgatat cgaggagggg cgggagcgca tcgaggctct
     7201 gcgcggcgaa atcgagacct gcagcggcga agtggtgcgc ggcgaggaag aggtgtcgca
     7261 attgcgcgag cggctcgccg agctcgaaca cgcgatctcg tcgagtcaac agcgcgggct
     7321 ggaattgaag gccgaggcgg agcggcacga acatcgaatt cagtacaacg aggagcgcct
     7381 gcgcgagttg gccgctcgga acgagaaggc gttggcggac atcgcggagg cggaggagcg
     7441 caagattgcg gcggagcggg cattggcgga gacggcggag cggctcgagt cggcagaggc
     7501 gaagctgggg gatgcgcggc gccagctcga agcccggcgc gaggggctga aggagctcga
     7561 ggatgacgca cggctcaagc aggaggcgat gcgccaggcc cagagcgatg tcttcgatgt
     7621 cgcccagcaa ctgacccgcg cgcgcaatga gatcaatgcc ctcgacctgc aaaaacaggg
     7681 caatgtcatc cgcatcgaga agctctccgc ggagaagatt cagctcgaag aggagcgcgc
     7741 cgggctcgcc aagcgcctcg aagagttcgc gcgcgaatcg gaagccgaac aggttgcgct
     7801 ccagacacgc cgatcgagtt tccaggagcg ccaggaacgc ctgcgcacgg tgcagcgcga
     7861 gatcgggcag gcggggctgg acctggacca actgcagcgc cggcaggccg agacccactc
     7921 gcgtctgaac gtcctccaac agcttgaatc cagccgcgag ggcttcggcg cgggcgcgct
     7981 cgccgcgttg aaggacaccc agcaggtgct cggctctctg gccgatcgca tccgtgtccc
     8041 ggacgaatac gttccggcca tcgaggccgc cttgggatcc cagttgcaga tcgtgctgac
     8101 gcactcgccg gacgtcgcgc gccagattct ggccgacttg agcgcccatc agaaaggacg
     8161 cgccagcatc gcgtcgctcg agcttgcgac cgcccgacgc gaggcgtcgc gcgacgccac
     8221 gagcccgccc attgccgctg agactgctcc agaagcagcg gcctgtcctc gagcggagga
     8281 cccgctcgaa gcgcgcgggt gcgtggccgc gctcggcgtg gtggaaggcg atgccgcggt
     8341 gcttcccttg ctcgaaagcc ttctcggacg gacccggatc gctcccgatc tcgaggccgc
     8401 gacgaccgcg tggcgggcct gcggcgggcg cttcgatttt gtcacgcgcg ccggagattt
     8461 gctgaatcgg cacggcgtct tcacgggcgg ttcgagcaac ggttcgagca aaggaccgtc
     8521 ctcgattctg gggcggaaga accagattgc cgaactcgag cggtcgctgg cggaggtcca
     8581 cgcccgcgtg aacgatgcga gccgccgcaa agggacgctg ctgagcgagc aaaccgcgtt
     8641 gcaggcgagc ctgcagcagg cccagtccga attgcaggcg caggaagtcg ccatcgccac
     8701 gcgtcagggc gagttcaagg ccctgcaaaa cgcccaacgc atgctcgaac agaaggtcga
     8761 caccgtcgtc ttcgagatcc aaaccctggc cggccaggag cgcgaaggca gcgagcggcg
     8821 cgccagcctg tccgcacagg tgggcgaact cgaagcgcgc gaccaccagg cgcagcgccg
     8881 gttgaccgaa ctcacaggcg cactcgaaca actccgcgtc cggcgcgacc agctcaacgc
     8941 cgccgtcacc gaggcgaagg tggcggtcgc caccgaggag cagcttgccg cctcgttccg
     9001 ccagcagcag gggccgctcg aacaacggat tcgcgaactc gcacatctgc tcgaacagcg
     9061 ccgccaggaa atcgcccagg tgctcgaccg ccgcggccag gcccagatgg aaatcgagga
     9121 ctcccgcgga cgcatcggcg ccgtccagca tgagagggac cagttgggcc tgcggacggc
     9181 cgatttgctg gcccagcgcg acgcccaggc ggacgacatc gccacgcgcg aagaggccct
     9241 gcgcaacagc cgccgccgcc tcaccgagca gcaagaactg cgcagcgcgg ccgaggtgga
     9301 cctggcccag aaagagatgg gcctgcgcaa catcgtcgag cgcatccagg agcgctacca
     9361 aatcaacctt gccgacgtcc gcagcgagtg catcaccatc acctatgccg ccgaaggacc
     9421 cgcccgggtc gagaccctga ctcccgagca aatggcggcc gcgggcgtct cgaccgactg
     9481 gaacgccgtg gccgagcagg tcgccgcact ccaggcaaag atcgacgaga tggggccggt
     9541 gaatctggtt gcgatcgagg aatacgagga gaccgagcaa cgccatcaat tcctgagttc
     9601 gcagcacgac gatctgatca aggccaagga acagctcgtc gaggtcatca accgcgtcaa
     9661 cacgcagacc cgccagatgt tcacggaaac cttccacaac atccgcgaca acttccgctc
     9721 gatctttatc gagatattcg ggggcggcgc ggcggatttg aacctggtgg acgaggggga
     9781 cgttctcgaa agcggcatcg agatcatggc gcgtccgcca ggcaaacagt tgcggagcat
     9841 ctcgctgctc tccggaggcg aacagaccat gacggccgta gcgctgctct tcgccatcta
     9901 ccaggtccga cccagcccgt tctgcgtcct ggacgaactg gacgcgccgc tggacgaatc
     9961 gaacatcgac cgtttcatcc gggtgctcca gcgctttctg ccccactccc agttcatcgt
    10021 gatcacccac aacaaacgca cgatcggcat ggccgatgtc ctctacggca tcaccatgca
    10081 agaacagggc gtcagcaaga tcgtcagcgt caggttccac aagaccgata ccccgattgc
    10141 cgaccgcgcc gccgcttcga tcgaaacgtc catcgatggc ccatctatcg gtgtcgagga
    10201 ggaccatgag atcggactcg aggaaacgct cgaagtgaca atcgccgagt agcaggagcg
    10261 catccaccct caccgccgca taaccgggcc gactctgact gccatcctgc cgctccactt
    10321 catgctttgg ccctgggcct gcaggcccgc tattaggatt gctctggggc aagacgacgg
    10381 tagcatccgg cgccgtgcaa aaccgcagcc gattgttgct ccgcgtggcg atgctgtgcc
    10441 tgttgctctc gggcatggcg ggtctggttt acgagattgt ctggacccgg tacctcgccc
    10501 tcttcctggg tcacaccagc tatgccgtca tcgccgttct cgtggctttt atgggcggcc
    10561 tggccttggg caacctctgg atcggcgtct gggccgaccg cacccggcga ccgctcgcca
    10621 gctatgcctg gctcgagatt gcgatcgggg tgtacgcgct gatcttccct atccactacc
    10681 agctctgtta cagcggcttc ctcggcctgg cccgctcgct cgatccgggt tccgccggac
    10741 ttctggcgct gaagttcctc ttcagcctgc tggccatcct catcccaacc gtgctgatgg
    10801 gaggaacgct gcctctgctg actcgcctcc tgacgcgctc cctgggcgag atgcgcgaga
    10861 aggtcgccgc cctctacttc atcaacagcg caggcggcgt catcggcatc tggctggccg
    10921 atttctggtg gatcggagcg atcggcctcc cggcaacgct catggtcggc gccgtcctca
    10981 atctcgccgt cggcggcctt gccctgtttg tcagcggcta catcaaggag gggcaagctg
    11041 accaagacga agccacgact cggctagccg acacgccgag accggaagcc gagccggaga
    11101 gctacgggcc cgtggaactg cgccttgcga tcctgggcat cggcctttcg ggctttgtcg
    11161 cgatgctgta ccaggtggcc tggacacgcc tgctggcgtt ggccatgggt tcgagcacgc
    11221 acgccttctc cctcatgctc atcacattca tcaccgggtt gaccgtgggg gcgtggattg
    11281 tgtatcgctg gcggtcgttg cgacggtccc tggcggcgtt tgcctgggcc gagttggccc
    11341 tggcactcac ggtcggcgtt tccctctttt tctacgaatt ccttccctat tggtttggcc
    11401 gcctggccga cctgctggcc cggcggcccg aggcctacag cctgtaccag ggtctgcagg
    11461 gactgctctg tttcgccgtc atgttcgtgc cgaccgtctg tctgggcatg actctgcccc
    11521 tggtaacgcg cgccgccacg ccctcgctcg aacgcgctgg gcgcaccgtc ggacgcgtct
    11581 ttgcagtcaa cacagtcggg accgttctgg gcgcggccct gaccgggctg gtgttgatgc
    11641 cgcgtctggg cctggctggc acgttcgtgc tcggggtggc cgtcaacctg ctgatcgggc
    11701 tggtcattgt cgtccgcgtc gaacgcggcg caaaacgcct gatcgcgatc ggggcgtccg
    11761 gcctcttcgt cgtgttcctg g
//
LOCUS       DAOQRO010000063        11771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4605_length_11771_cov_4.168914, whole genome shotgun sequence.
ACCESSION   DAOQRO010000063 DAOQRO010000000
VERSION     DAOQRO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4605_length_11771_cov_4.168914"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1417
                     /locus_tag="PKK20_03790"
     CDS             164..1417
                     /locus_tag="PKK20_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNU99040.1"
                     /translation="MLALPLLVIAGFLAFAGASFAFALAETALFTLGKWQTEQLAERE
                     GDRGRAVAHLLAMPQDLLATIVLGNTFASAGLVSLGLWVVLNEEWPPLATSFGVLVLI
                     LVGGEVIPKTLAVRSPQFWALRVARPMALLQTLTRPLHWLARQFNTQVLRWTVPESMR
                     LPVTGGDEEYAELLELGFQQGALAQSEKEIILQIINLDRHTAKDVMRPRAQMHALPDD
                     LPVSEMIAAARRYKHRRLPLYDETPDTIVGILNTRELLLNPEEDLSEMVDFPSFVPET
                     TNLLRLLQSLQRQRRGVAIVVDEYGSTSGLVTTEDILGSMVGRIQGEGEAEGFVMERL
                     GPGRWRVNGTMRLEDFRREYPELGEAPDVDTMGGLLVALAEVVPPAGHAVQFRNLRLR
                     ATQVDDRRVRELVVEDCRKTGGAAI"
     gene            1435..2394
                     /locus_tag="PKK20_03795"
     CDS             1435..2394
                     /locus_tag="PKK20_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNU99041.1"
                     /translation="MLLLLLVAALLFLFSGMEAGVFALSRLRIRQQMRAGSTSARILH
                     QFLEQPEQFLWTILVGSTLAEFLLVGLTFLYLHRWLAGGVWLWLALAAAVFLIYALAD
                     LLPKMLFRQYSNRLCLGLARLFRITHLTLAPLVAIVAWLAEVLLKSTGGHRFTGRLFA
                     NREELRYMMQESSHALSKEERTMVNRVLDLQNLTVGAVGTPLSKVVTVTTTTPIAEVF
                     KVCRETGLNRMPVKDPANGRVVGIVTLRNSLYQHDLDERKPVGAFLQPALYLEESARL
                     EVALQRLRQSGQRIAIVLDRNRKEAGIVTLHDILRHIFGEVSW"
     gene            2396..3721
                     /locus_tag="PKK20_03800"
     CDS             2396..3721
                     /locus_tag="PKK20_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNU99042.1"
                     /translation="MNSEGYSLTLLKLVAVLGLVCLNGFFVAAEFAFVRLRRTQLDAL
                     VAKGHRRARIARRLATNLESCISTVQIGITLCGIATGALVKPVFEALLRPVFQTLAVE
                     SPAIRTTVEFSIGFLVSTFLLIVVGELVPKSLAIRKTLAVSLWTARWLEMFYYLTRPF
                     IWILNRSRGWILGKLGVAASSESEHGHSEEELRLLVSTATSRSGETALSRNLVLNALD
                     IRQRIVREVMRPRREITALDTRGSLTECIEIAEWSRYSRFPLCEDGDLDRTVGVVHIK
                     DLYASRHKARTGGELRPVARRLIYVPETARLERVLGLFLERRLHLAIVVDEYGSTVGM
                     VTLENLLEELVGQIQDEFDQEKPLFIQTSDQTWEVDGILPLHELSDLVGAPLREEGIT
                     TTSGLVTQRLGGFPKPGDTLTLGHYELRVEETDGPRVSKLQLTKRSPTQ"
     gene            complement(3764..4660)
                     /locus_tag="PKK20_03805"
     CDS             complement(3764..4660)
                     /locus_tag="PKK20_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNU99043.1"
                     /translation="MDPLDAQRIAPEALRLPARQATAPLPQAQLCFRIAFAASIVSGV
                     VCLSAALAGHGSSGVGRWSAGAFFVFSSFAILCCLGHRLPPQNLCAVLLVSWASASLG
                     LMLAVKSHVFLDAVTYADGFGPRMLGIVPWQLPFLWLALVLSSRETARLILRPYRRDP
                     HYGYWLVGLAAVLVLGVDLVLQPFAVRVAGWWRFETSPEASSWLGVPWVVFPTNIALT
                     LLVLLFAGPWLIAKRAIPTARNLCPLMVWLFLNLCLLIGSSVAGLWLAMAASLAGMAA
                     AVFFARRARTAFPTMPDETART"
     gene            complement(4709..6910)
                     /gene="glgB"
                     /locus_tag="PKK20_03810"
     CDS             complement(4709..6910)
                     /gene="glgB"
                     /locus_tag="PKK20_03810"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNU99044.1"
                     /translation="MILTPAELESLVNARHRTPHDLLGMHLLGDGSGIVVRALLQDAA
                     QVEIVPTIEKDKPSFTLARLHETGLFEGASDKSKEIYAYDLVITDFAGRRRQMRDAYS
                     FLPTLEEIDLFLFNQGNERRLYDKLGAHQRVLDGVPGVGFSVWAPNAQRVSVVGDFNH
                     WDGRYHPMRLLGVSGVWELFIPGLDEGVHYKFEVRNAHGLPVLKADPFATRFEVAPKN
                     ASLVWNTRKFAWTDDAWMERRRQCNPFQSPLSIYEVHLGSWRKQAVGQSLGYRDLASP
                     LVDYVQRMGFTHVEFLPVAEHAYYPSWGYQVTGFYAPTSRYGAPEDFQYLVNALHAAG
                     IGVIVDWVPAHFPRDDWALARFDGTALYEHEDPRQGTHQDWGTYVFNFGRHEVRNFLT
                     ANALFWCERYHIDGLRVDAVASMLYLDYSRKEGEWIPNRYGGRENLEAIEFLREFNHI
                     IHTEHPGVLTIAEESTAWPQVTRPPYLGGLGFSFKWNMGWMHDTLDYFRRDPVFRKYH
                     QNDLTFAMLYHHHENFILPLSHDEVVHGKGSLRGRMPGDDWRGFANLRALLAYQWLFP
                     GKKLVFMGAEIGQKHEWDANGQVDWWLLEAGPYHVGVQRFLADLNGVYRKHAAFWEGD
                     HDPRGFQWIDCADHENSVLSFLRRPLGSGREFVIVLNLTPVVRGNYRVGLPRGGFWTE
                     VLNSDATVYGGSNQGNAGGVHAESVPAHQQQFSARLTLPPLGVVVLGSDEP"
     gene            complement(7039..11706)
                     /locus_tag="PKK20_03815"
     CDS             complement(7039..11706)
                     /locus_tag="PKK20_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNU99045.1"
                     /translation="MSPVDDRLREDPEYIVVTILPGTEYNVGAQGQAVIVLNDDDGGS
                     ALAVGFDFLTSSVFEGTSAGEAYLAVTVTGNPAQDQDVTVQYKVTGGTAIPDVDYPLA
                     SSTGLVVFPHNPNGAEDSRWVERTKLIALPINDNQLIEPDRTIVVTLLEPPSWLSNWL
                     TTNDITLTNEVGEPVTTNEVVTNYMFVPIPFNAQFDVYKTHTLTILDDDSAIVTIEAG
                     APQVDEGDPVGTYFRIRREGPTNRVQKVVLQTSGFASNGSDYQPIPSTVEIPIGEAWI
                     DVPLLPVDDPVQEFMEDVTVTLVDVPGGTIGGQSSATIQIVDNDGTVEFTQTAYTVSE
                     NVGTARVPVRRTGHNDTPLVVNYSVLGVSATPGADFVATNGTFTFAPGETVFDLPVII
                     LDDHVVEPAETVRLILTSLGSGAPIGGQTYATLTILDDDSYVEFAAANYRVTENGTNA
                     IIALHRIGAVDNPIEVDFAATNGTATDGIEFVATNYPVAFAPGVTNATVLVRILDNTL
                     IETNKTVHLALATNAVSGGLVGPQATALLEIVNDDCALEFASSEYSVDEFARVVTLDV
                     RRIGGTVNAVSVDYTTTNGTAQATKDYVATAGTLRFAGETNVVLQGGSGNFVLEPGET
                     NKQFQVRVIDNSAGEGNRSFGVLLSNPRGPATGALPGSTILGEQANTIVTIVDDELPG
                     SVDFAFNPGLGADGPVNAVAVQPDGKIVLAGAFATVDGTVMPRVARLHEDGYLDSFFN
                     PGAGPNDTVHAVAVQPDGRILLGGEFTTYSDDNTPRIARLAADGFIDSSFNPGAGANR
                     TVRALAVGANGAIVLGGDFLTVGGVSRPGIARLIADGSLDRTFDPGAGAPGGVFTVAI
                     QSDGKVLAGGGFSSAGGANYPFLARFNADGTVDRSFNTGTGPDGAVRSIAVGADGKVI
                     IGGAFVKFNNVNRARIARLNADGSLDASFDPGSGANQTVYGVGVQTDRKVVLAGNFTE
                     VRGQLMSRYARLNPNGTVDDGFLIFQGADGLIRSLALQSDTALVVGGDFTTLNGLPRR
                     GVGRVHGDEKFILSKIQFSSAAYRVVEDQGPATVTVVRSGDLAASAQVDFLTSGGSAV
                     PGLNYQPTNGTLLFLPGETERTFEVQAMDDHAARGNVTVGLVLTNLPSGYALNAQLTS
                     VLTIEDKESVLAFSSANYSVLRSESEATIAVVRTGPSTNATSVDYVSQDDTAQANIDY
                     LPVSGTLVFDPGVMLQTFTVPILTNSIADEPKTLVLALANAGSGAVLGALSNAVLSIV
                     NEVVEFYSLNLTPPVGGSVTPGSDQYPAGSSQILTATPDEDYRFDGWEGTTNTSANPL
                     QLVMNRNYTLTARFVPTKTTYSFEPPFRASDLASPPWANSTTAPWQLQSLSAAGGRYA
                     LRSGVIGNSQETRLELTARTRAGAVSFMVRVSSEANWDFFEVYINGVRLQRWSGDLPW
                     QRYQFNVPAGVNRFTWRYVKDANFNSGLDAAFIDDIYLPLDIPDPTPVEPVLSVAGFN
                     GGVITIKLEGRRGVTYVLEASEDLKVWTPIGTETLQTPSTLFFDTVTGNHPARFYRAL
                     TPD"
BASE COUNT         2203 a         3325 c         4022 g         2221 t
ORIGIN      
        1 tggctcgcac ggaagccgtt gatccgacgc cacacacaac cctgtcgtga tgacctcggc
       61 ttcgccgtcg ccgtcggtcg taacttggtg aggcaaggtg gttcgagcag aaggccggtt
      121 cctgggcttg tagacgccag tagggacggg catactcggg acgatgttag cgctgcccct
      181 tctggtgatt gcgggctttc ttgcttttgc cggtgcgagt tttgcgttcg cactggcaga
      241 gacggccctc ttcaccctgg ggaagtggca gactgagcaa ctggccgagc gggaggggga
      301 tcgggggcgg gcggtggcgc acctgctggc aatgccgcag gatttgctgg cgacaatcgt
      361 tctgggaaac acgtttgcga gcgccgggct ggtgagtttg gggctgtggg tggtcctcaa
      421 cgaggagtgg ccgcctttgg cgacgtcctt tggcgtcttg gtcttgattc tggttggagg
      481 cgaggtgatt ccgaagacgc tggcagtgcg atcgcctcag ttctgggcgc tgcgggtggc
      541 gcgtccgatg gcgctgctgc agacgctgac ccgtcctctg cattggctgg cccgtcaatt
      601 caacactcag gtgttgcgat ggaccgtgcc ggagtcgatg cggttgcccg taacaggggg
      661 tgacgaggag tacgccgagt tgctcgaact cggtttccag cagggtgcgt tggcgcagtc
      721 ggagaaagag atcatcctgc agatcataaa tctggatcgc cacacggcga aggacgtcat
      781 gcgtcctcgt gcgcagatgc acgcgctgcc ggacgacttg ccggtttccg agatgatcgc
      841 ggccgcccgg cgctacaagc accgacgatt gcccctttac gacgagacgc ctgacacgat
      901 tgtcgggatt ctcaacacga gggagctgct gctcaatccg gaggaggatc tttccgagat
      961 ggttgacttt ccgtcattcg tgcctgagac cacgaatctg ctgcgactgt tgcagagcct
     1021 acaacggcag aggcgcggtg tggccatcgt ggtagacgaa tacgggagca catcggggct
     1081 ggtgacgacg gaggacattc tcgggtcgat ggtggggcgc atccagggag aaggcgaggc
     1141 ggaggggttc gtcatggaac ggctgggacc aggccggtgg cgggtgaacg gcaccatgcg
     1201 cctggaggat ttccgtcggg agtaccccga gttgggagaa gcgccggatg tcgacacgat
     1261 gggcggactt cttgtggcgt tggccgaggt ggtgccgcct gccgggcatg cggtacaatt
     1321 ccgcaatctc aggctaaggg cgacgcaggt ggacgaccga cgcgtgcggg agttggtcgt
     1381 cgaggattgc aggaagacag gaggagcagc gatctaatgg gacacgtgtg gaccatgctg
     1441 ttgctgttgc tggtggcggc cctgctgttc ctgttttcag ggatggaagc gggtgtattt
     1501 gccctgagcc ggctgcgcat tcggcaacag atgcgcgccg gcagcacctc ggcgcgcata
     1561 ttacatcagt ttctagagca accggagcag tttctgtgga cgattctggt tggcagcacc
     1621 ctggcggagt ttctgcttgt gggcctgacg tttctgtatc tgcatcgttg gctggcgggg
     1681 ggggtatggc tctggctggc cctggcggct gccgtgtttc tcatttacgc tctggcggat
     1741 ttgttgccca agatgctgtt tcgtcaatac tcgaaccggc tttgcctggg cctggctcgg
     1801 ctcttccgca taactcatct caccttggcg ccgctggtcg caatcgtcgc ctggctggcg
     1861 gaggttcttt tgaagtcgac cggcggccat cggttcacgg gacggctttt tgccaaccgg
     1921 gaggagctgc ggtatatgat gcaggagagt tcgcatgcgc taagcaagga agagcgcacg
     1981 atggtcaatc gcgtgttgga tcttcagaat ctcacggttg gggctgtagg aactccgctg
     2041 agcaaggtgg tcacggtgac gacgacgaca ccgatcgcgg aggttttcaa ggtgtgccgc
     2101 gagactgggt tgaatcggat gccggtgaag gatccggcca atgggcgggt tgttgggatc
     2161 gtgactttgc gcaactcact ttaccaacat gatttagacg aacgcaagcc agtcggagcc
     2221 tttctgcaac cagcgctcta cctcgaggag tcggcgcggc tcgaagtggc tctccagcgt
     2281 ttgcggcagt cgggccagcg cattgccatc gtgctggatc ggaaccggaa ggaagccggg
     2341 attgtgacgt tgcacgacat ccttcgccac atctttggcg aggtgagttg gtgagatgaa
     2401 cagcgaggga tacagtctga cattgctcaa acttgtggct gtccttggct tggtttgtct
     2461 gaacggtttt ttcgtcgcgg ccgagtttgc gttcgtgagg ctgcgaagga cgcagttgga
     2521 tgcgctggtc gcgaaggggc atcgtcgagc ccgcatcgct cggcgtttgg ctactaacct
     2581 ggaatcctgc atcagcaccg ttcagatcgg catcacgctg tgcgggattg cgaccggagc
     2641 tctggtgaag cctgtgttcg aggcgctgct gcgtccggtg tttcagacgc tggcggtcga
     2701 gtcgccggcg attcgaacga cagtcgagtt ttcgatcggg tttttggtca gcacgtttct
     2761 gctgattgtg gtgggcgaac tggtgcccaa gtctctggcg attcgcaaga ccctggcggt
     2821 ctcactctgg acggcgcgct ggctcgaaat gttttactac ctgacacggc cgttcatctg
     2881 gattctgaac cggtcgcgcg gctggattct gggcaagctt ggggtcgcgg cgtcgagcga
     2941 atcggagcac ggccattccg aggaagagtt gcggctgctc gtttccacgg caacgtctcg
     3001 gagcggggag acggctttga gcaggaacct ggtgctcaat gcgctcgata tccgccagcg
     3061 catcgtgcgc gaggtcatga gaccgaggcg cgaaatcacg gccctcgata cgcgcggcag
     3121 cctgacggaa tgcatcgaga ttgccgagtg gagccgatac tcgagattcc cgctctgcga
     3181 ggatggagac ttggaccgga cagtaggtgt tgttcacatc aaggatctct acgcgtcgcg
     3241 gcacaaggcg cgtaccggag gcgaattgcg accggtggcg cgcaggctga tctacgtgcc
     3301 ggagacggcg cggcttgagc gcgtgttagg gttgtttctc gaacggcggc tgcacctggc
     3361 gatcgtggtg gacgagtacg ggagcacggt ggggatggtg accttggaga acttgctcga
     3421 agaactggtc gggcagatcc aggacgaatt cgatcaggag aagccccttt tcatccagac
     3481 gagcgaccag acttgggagg tggacgggat cctgcctttg cacgagctgt ccgatctggt
     3541 cggagcgccg ctgcgagagg aggggatcac gaccaccagc ggtctggtca cacaacggtt
     3601 gggcggcttt ccgaagccgg gcgacactct cacactgggt cattacgaac ttcgcgtcga
     3661 ggagaccgat ggcccgcgcg tgagcaagct gcagttgacc aagcggagcc cgacgcaatg
     3721 agagggcggg ctgaggatcg gaattggccc ggggcgggcc ttgctaggtc cgcgcagtct
     3781 cgtcgggcat cgtcggaaac gccgtgcggg cgcgccgtgc gaagaagacc gcggcggcca
     3841 ttcctgccag agatgcggcc attgcgagcc agagtccggc cacggagctt ccgatgagaa
     3901 ggcagaggtt caggaagagc cagaccatca gggggcaaag attgcgggcg gtcgggatgg
     3961 cgcgtttggc aatgagccaa gggccggcga agaggaggac gaggagcgtt agagcgatat
     4021 tcgtgggaaa caccacccag ggaacgccca gccaactcga ggcctcgggt gaggtctcga
     4081 atcgccacca tccggccacg cggaccgcaa acggctgcaa caccaaatcc acgcccagga
     4141 ccaggaccgc ggcaaggccg accagccagt atccgtagtg ggggtcgcgt cggtaggggc
     4201 ggaggatgag acgggcggtc tcgcggctgc tgagcaccag ggcaagccag aggaagggca
     4261 actgccacgg cacgatgccg agcattcggg ggccgaatcc gtcggcgtac gtcactgcgt
     4321 ccaggaagac atgggacttg acggcgagca tgaggccgag agaggcgctg gcccaggaga
     4381 cgagcagaac agcacacagg ttttgcggag gcaggcggtg gccgaggcaa cagaggatcg
     4441 cgaagctcga gaagacgaaa aaggcgcccg cagaccaacg tccgacgccg ctggagccat
     4501 ggccagcgag cgctgcgctc aagcagacga cgccgctcac aatcgacgcg gcgaatgcaa
     4561 tgcggaagca gagttgcgcc tgcgggagag gcgccgtggc ctgccgtgca ggcagcctga
     4621 gcgcctcagg cgcgatccgc tgggcatcca gggggtccat aaaacccaga cgctatcgga
     4681 aggggagagg catgggaacc tcaaaatgct aaggctcatc ggagccgagc acgaccacgc
     4741 cgagcggcgg gagcgtcaac cgggcggaga actgctgctg atgagccggg acggattctg
     4801 cgtggacgcc gccggcattg ccctgattgc tgcctccgta gactgtcgcg tcgctgttga
     4861 gcacctcggt ccagaacccg ccccgcggaa ggccgacgcg gtagtttccg cgcacaaccg
     4921 gagtcaggtt caagacgatc acgaactcgc ggcccgatcc aagagggcgc cgcaggaagg
     4981 agaggacgga attctcgtgg tcagcgcaat cgatccactg gaagccgcgg ggatcgtggt
     5041 cgccttccca gaaggcggcg tgcttgcggt acacgccatt gagatcggcg aggaaacgct
     5101 gcaccccgac atggtagggg cccgcttcga gcaaccacca atccacctgg ccattggcgt
     5161 cccactcgtg cttctgaccg atctcagccc ccatgaacac gagcttcttg cccgggaaaa
     5221 gccattggta agcgaggagc gcgcggagat tggcgaagcc ccgccaatcg tcgccgggca
     5281 tgcgtccgcg gagggagccc ttgccgtgca cgacctcgtc gtgggagagg ggcaggatga
     5341 aattctcgtg gtggtggtag agcatcgcga acgtcagatc gttctggtga tacttgcgga
     5401 agacgggatc acgacggaag tagtcgagcg tgtcgtgcat ccagcccatg ttccacttga
     5461 agctgaagcc gaggccgccg aggtagggag gccgggtcac ctggggccag gcggtggatt
     5521 cctcggcaat ggtcaacacg cccgggtgtt cggtatggat gatgtggttg aactctctga
     5581 ggaactcaat cgcttcgagg ttttcccgtc ctccgtagcg gttaggaatc cactcgcctt
     5641 ccttgcgtga gtaatcgagg tagagcatgg acgccacggc atccacgcgg aggccatcga
     5701 tatggtatcg ctcgcaccag aagagcgcgt tggccgtgag gaaattccgg acttcgtggc
     5761 ggccgaagtt aaagacgtag gtgccccagt cctggtgagt cccctgtcgc ggatcctcgt
     5821 gctcgtacag ggcggttccg tcgaaacggg ccagggccca atcatcgcgt gggaaatggg
     5881 ccggcaccca atccacgatc acaccgatac cggccgcgtg cagagcgttg acgaggtatt
     5941 gaaagtcctc cggggcgccg tagcggctgg tcggtgcgta gaagccggtg acctggtatc
     6001 cccagcttgg gtagtaggca tgctccgcca cgggaaggaa ctcgacatgc gtgaagccca
     6061 tccgctggac gtagtcaacg agggggctgg ccaggtcgcg gtagccgagc gactggccga
     6121 cggcctgctt gcgccacgat ccaaggtgga cttcgtagat cgagaggggc gattgaaacg
     6181 gattgcattg gcgtcgtcgc tccatccagg cgtcatcggt ccacgcgaac ttgcgcgtat
     6241 tccagacgag gctcgcgttc tttggcgcca cttcgaagcg cgtggcaaac gggtcggcct
     6301 tgagcacggg cagtccgtgc gcattgcgca cctcgaactt gtaatgcacg ccctcgtcca
     6361 ggcccgggat gaagagctcc caaaccccgg atacccccaa caaacgcatc ggatggtagc
     6421 gtccgtccca atggttgaaa tcaccgacca cgctgaccct ctgcgcattc ggagcccaga
     6481 cagagaaccc gacgcccggc acgccatcaa ggacgcgctg atgggcgccg agtttgtcat
     6541 acaagcgtcg ctcgtttccc tggttgaaga ggaacaagtc gatctcttcc aaggttggaa
     6601 gaaatgaata tgcatcgcgc atctgacggc ggcggcccgc gaagtccgtt atgaccaagt
     6661 cgtaggcgta gatttccttg cttttgtccg aggccccctc gaacaacccg gtttcgtgca
     6721 gcctggccag cgtgaacgac ggtttatcct tttcgatcgt gggcacgatc tcgacctgtg
     6781 cggcgtcctg aagcagggcg cgcaccacga tgccggaacc gtcccctagc aggtgcattc
     6841 cgagcaaatc gtgaggcgtc cgatgccggg cattgaccag gctctcgagt tcggcaggcg
     6901 taagtatcat cgtgtctgca atctgccaca gggctgcacg gaaaagcact gaaaaaaccg
     6961 caaaccgtgt cgcgatcgcg acacgaaatc tgcaccatgc gcaggtggcg gtgtgagctg
     7021 gcgccgagcg gacttcgatc agtcgggggt cagggcgcga tagaagcgtg ccgggtgatt
     7081 gccggtgact gtgtcgaaga acagagtgct gggggtttgc agcgtttcgg tgccgatcgg
     7141 tgtccaaacc ttgaggtcct cacttgcttc gaggacgtag gtgacgccgc ggcggccctc
     7201 gagcttgatg gtgatcacac cgccgttgaa acctgcgacg gacagcacgg gctcgacggg
     7261 cgtcgggtcc ggaatatcca gcgggaggta gatatcgtca atgaacgcgg catccagtcc
     7321 ggagttgaaa ttggcatcct tgacgtaacg ccaggtgaag cggttgacgc cggcgggcac
     7381 attgaattgg tagcgctgcc aaggcaggtc gccggaccag cgttggagcc gcacgccgtt
     7441 gatgtagacc tcgaagaaat cccaattggc ttccgaactc acccggacca tgaaggacac
     7501 ggcgccggcg cgggtgcggg ccgtcaactc gaggcgggtc tcctggctgt tgccgatcac
     7561 acccgagcgc agggcgtagc gcccgccggc ggccgagagg gactgcaact gccacggggc
     7621 ggtcgtcgag ttggcccagg gaggcgaggc gagatcggac gcccgaaacg gaggctcgaa
     7681 ggagtaggtg gtcttggtgg gaacgaaccg cgctgtgagc gtgtagttgc ggttcatcac
     7741 gagctggagc ggattcgcgg acgtattggt cgtcccttcc cagccgtcga aacggtagtc
     7801 ctcatccggc gtggcagtga gaatctggga cgagcctgca gggtactgat ccgatccggg
     7861 agtcacggag ccgccgaccg gcggcgtgag gttgaggctg tagaactcga cgacctcgtt
     7921 cacgatcgac agcacggcgt tgctgagggc gccgagcacc gcgccgctgc cggcattggc
     7981 gagggccaga acgagggtct tgggctcgtc ggcgatgctg ttggtaagaa tcggcaccgt
     8041 gaaggtctgc agcatgacgc ctgggtcgaa gaccaatgta ccgctgacgg gcaggtagtc
     8101 gatgttcgcc tgggcggtgt cgtcctggct cacgtagtcg acgctcgtcg cgttcgtgct
     8161 ggggccggtg cggacgacgg cgatggtggc ctcgctttcg gatctgagga cggagtaatt
     8221 ggcgctgctg aaggcgagga cgctctcctt gtcctcaatg gtcaggaccg aagtcaattg
     8281 ggcattgagc gcgtagcccg acggcagatt ggtcaagaca agcccgacgg tcacattgcc
     8341 gcgggcggcg tgatcgtcca tggcctggac ctcgaaggtc ctttccgtct ctcctggcag
     8401 gaacagaagc gtgccgttgg tgggctggta attgaggccc gggacggcgg agccgccgga
     8461 ggtgaggaaa tcaacctgag ccgacgcggc gaggtcgccg ctgcggacga cagtgacggt
     8521 ggccggcccc tggtcttcaa ccacacgata ggcggcggag gagaactgaa tcttgctgag
     8581 gatgaacttc tcgtcgccgt ggacgcgacc gacaccccgt cgcggtaagc cgttgagcgt
     8641 ggtgaagtcg ccgccgacaa cgagggccgt gtcggactgg agcgccagac tccggatcaa
     8701 cccgtcggcc ccttggaaga tcaggaatcc gtcatccacg gtgccgttgg ggttgaggcg
     8761 cgcgtagcgt gacatgagct ggcctctcac ttcggtgaaa ttgccggcca gaacaacctt
     8821 cctgtcggtt tggacaccga cgccatacac ggtctggttg gcgcccgagc ccggatcgaa
     8881 gctggcatcg agagacccgt ccgcgttgag ccgggcgatg cgcgcacggt tcacattgtt
     8941 gaacttcaca aaggcccctc cgatgatgac cttgccatcc gcgcccaccg cgatggatcg
     9001 tacagcccca tcgggcccgg tccccgtgtt gaaggaccgg tcaacagttc cgtccgcatt
     9061 gaaacgggcc aggaaggggt aattggcccc gccggcactc gagaagccac caccggccaa
     9121 caccttcccg tccgactgga tggcgaccgt gaagacgcct ccgggcgcgc ccgcacccgg
     9181 atcgaatgtc cgatcgaggg agccgtcggc aatcagacgg gctatcccgg gccggctgac
     9241 gccgccgacc gtgagaaaat cgccgccgag gacaatggcg ccgttggctc ccacagccag
     9301 tgcgcggaca gtgcgatttg cacccgcacc cggattgaag ctggaatcga tgaagccgtc
     9361 agccgccaag cgggcgatgc gtggcgtgtt gtcgtcgctg taggtggtga attctccgcc
     9421 cagcaggatg cggccatccg gttggacggc gacggcatgc acggtgtcgt ttggacctgc
     9481 gcctggattg aagaagctat cgagatagcc atcctcgtgc aggcgggcga cgcgcggcat
     9541 gaccgtgcca tcgacggtgg cgaatgcgcc ggccaggacg atcttgccat ccggttgcac
     9601 ggcgacggcg ttgaccggcc catcggcgcc caagcctgga ttgaaggcga aatcgacgct
     9661 gccgggcaat tcgtcgtcga cgatggtgac gatggtgttc gcttgctcgc ccaggatcgt
     9721 cgagccaggc aatgccccgg tggccgggcc tcggggattg ctgaggagga ctccaaaact
     9781 ccggttgcct tccccggcgg aattgtcgat gactcggacc tggaactgct tgttcgtttc
     9841 gccgggttcg aggacaaagt tgcccgagcc gccctggagc acgacgtttg tttcgccagc
     9901 gaatcggagt gtgcctgcgg ttgcgacgta atcctttgtc gcctgggcgg tcccgtttgt
     9961 cgttgtgtaa tcgaccgaaa cggcgttgac cgtgcctccg atgcggcgca cgtccagcgt
    10021 gacgacgcgc gcgaactcat ccacgctgta ttcgctcgag gcgaattcga gagcgcagtc
    10081 gtcgtttacg atttcgagca aggccgtggc ctgaggcccg accaacccgc cgctgacggc
    10141 gttggtcgcg agcgccaagt ggacggtctt gttcgtctcg attagcgtgt tgtcgaggat
    10201 gcgcaccagg acggttgcgt tggtgacacc cggagcgaac gcgaccggat aattcgtggc
    10261 cacgaactcg atgccatcgg tcgccgtgcc gttggtggcg gcaaagtcga cttcgatcgg
    10321 attgtcgacc gccccgatcc tgtgcagcgc gatgatggcg ttcgtcccgt tttcggttac
    10381 gcggtaattg gctgcggcga attcgacata ggagtcgtca tcgagaatgg tcaaggtggc
    10441 gtaggtctgc cccccgattg gggcgccgct gcccagactg gtaaggatca gccgcaccgt
    10501 ttcggcgggt tcaaccacat ggtcatccag gatgatgaca ggcaggtcga aaacagtctc
    10561 gcccggggcg aacgtgaacg tgccgttggt cgccacgaaa tcggctccgg gcgtggcact
    10621 cacgccgagg acggagtaat tgacgaccag aggggtgtcg ttgtgaccgg tgcgccgaac
    10681 cggcacgcgg gctgtgccaa cgttttcgga cacggtgtag gcggtttgag tgaactcgac
    10741 cgtgccatcg ttatcaacga tctggatcgt ggcactgctt tggccgccga ttgtgccgcc
    10801 gggcacgtcg accagcgtca ccgtcacgtc ttccatgaat tcctgaaccg ggtcgtcgac
    10861 cggcaagagc ggaacatcga tccatgcctc gccaatcggg atttcgaccg tcgatgggat
    10921 aggctggtaa tcgctgccgt tgctggcgaa cccggaggtt tggaggacga ctttctgaac
    10981 tcggttcgtg gggccctcgc gtcggatgcg gaagtaggtg cccacgggat ctccctcgtc
    11041 aacctggggc gctcccgcct cgatcgtcac gattgcggaa tcgtcatcga ggatggtgag
    11101 agtatgcgtc ttatagacat cgaactgggc gttgaagggg atcgggacga acatgtaatt
    11161 cgtgacgact tcattggtgg tgaccggctc acccacttcg ttggtgagtg tgatatcgtt
    11221 ggtcgtcagc cagttgctga gccatgacgg aggttcgagg agcgtgacca caatcgtccg
    11281 gtccggctcg ataagctggt tatcgttgat cggcagggcg atcagcttgg tccgctcgac
    11341 ccatcgactg tcctcggcgc cgttgggatt gtgcggaaaa accaccaggc ccgtcgagct
    11401 cgccagcgga taatcaacgt cggggattgc ggtgccgccg gtgaccttat actgaaccgt
    11461 gacgtcctga tcctgggcag gattcccggt aacggtgact gccagatagg cttcgccggc
    11521 gctggtgcct tcgaacacgc tcgatgtcag gaagtcgaag ccgacggcga gcgctgaacc
    11581 gccgtcgtcg tcgttcagaa caatcacggc ctgaccctga gcacccacgt tgtattcggt
    11641 tccaggcagg attgtcacca cgatgtactc gggatcctcg cgcaaccggt catccactgg
    11701 ggacaccgag acaaccaccc gattctgggc cgccgggaat cgaatccgcg aaatcgggat
    11761 gtaatccgca a
//
LOCUS       DAOQRO010000064        11709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4658_length_11709_cov_5.299382, whole genome shotgun sequence.
ACCESSION   DAOQRO010000064 DAOQRO010000000
VERSION     DAOQRO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_11709_cov_5.299382"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..536)
                     /locus_tag="PKK20_03820"
     CDS             complement(111..536)
                     /locus_tag="PKK20_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99046.1"
                     /translation="MTGTLSTTPGGDRCEVRNVAAAAGPPFSKVWKASARIFQRLENE
                     CPQVSNVWKFPDTYARARGRAALAWGGGWGGLEIFADVPADHTAAVIRALAPAFGVAL
                     VGGCGWFPLVVWLALGHRISRRSSRFSADAKNGRHAGNP"
     gene            complement(1187..2712)
                     /locus_tag="PKK20_03825"
                     /pseudo
     CDS             complement(1187..2712)
                     /locus_tag="PKK20_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199726.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA+ family ATPase"
     gene            complement(2721..3713)
                     /locus_tag="PKK20_03830"
     CDS             complement(2721..3713)
                     /locus_tag="PKK20_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNU99047.1"
                     /translation="MKTIAFFNNKGGVGKTTLVYHLAWMYADLGVSVVAADLDPQANL
                     SAMFLSEERLEELWPDGDHPKSILGTISPILRGLGDIAEPHVEKINRDLGLLVGDLGL
                     SRFEAKLSSAWPDCMNHDEAGFRTESAFHRAILKAAEAREAELVLIDVGPNLGAINRA
                     AIIAANYVVFPLAPDLFSLQGLRNLGPTLQDWRNEWRDRLGRPPADRNLLLPSADIKP
                     AGYVVMQHAVRADRPVKAYDKWMQRIPAVYHQEVLHDRGTSARSTAEDPECLATLKHF
                     RSLMPMAQDALKPMFHLKAADGALGGHIYAVQECHADFKKLAVAIATKCGVSIP"
     gene            complement(3710..4894)
                     /locus_tag="PKK20_03835"
     CDS             complement(3710..4894)
                     /locus_tag="PKK20_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99048.1"
                     /translation="MLTTAQLQALMADTESDRVERTISTKNTDKFAEAICAFANDFPN
                     HRQPGYLLVGVNDEGRAGGLTVTDALLRDLANLRSNVNLEPIPALTVQKKQLPDGEVA
                     VVEVIPSNLPPVRYKGRVWIRVGPSRRAANQQEERILTEKRTAFQKTFDGRPCEGCAL
                     DDLVLDLFLVTYLPAAVAREVIEENKRDIRQQMASLRFFDLGADCPTHAGALLFAKEP
                     LRWVPGAYIQFVRWGGTTMADNPLNTKQFSGDLLTVLREMQAFLSLPAAAAPVAESAL
                     RERTETDYPTVAIRELLWNAVMHRSYESNAPVRFYWYDDRIEIQNPGGLYGMASPENF
                     PKQTDYRNPVVSEAMATLGFVNMFGRGVIRAQDALRRNGNPPAEFTFETSHVLATIRR
                     KP"
     gene            complement(4845..5504)
                     /locus_tag="PKK20_03840"
     CDS             complement(4845..5504)
                     /locus_tag="PKK20_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNU99049.1"
                     /translation="MSKPWERAEGYRRREDTRVIRNRILIVCEGEKTEPNYFRKFSVD
                     IDLVEVDVQGIGANTLSLVGDAVARRDDASREGTPYNQVWCVFDRDVFPAGNFNEAFR
                     LARTNRIRVAYSNQCFELWYFLHFNFSDAALHRQAYGDKLTGLLGRKYQKNDTKMYDL
                     LKDRQATAMQNARKLLTRYVPCNPEKHNGPRQTTSTNLKREHANVDNGTTASADGRHG
                     I"
     gene            complement(5497..6831)
                     /locus_tag="PKK20_03845"
     CDS             complement(5497..6831)
                     /locus_tag="PKK20_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99050.1"
                     /translation="MFIIVLNGYSGILELWESSMLIEFNVTNFLSFKNRATFSMVAST
                     DDTLEESNVIATEKHRLVRSAVVYGANASGKSNLLAALNLMRRMVLTSSKDTQVNEEI
                     DVEPFLLSTECDGKPSQFEVVFLQGGTLYRYGFQTDRKRVHAEWLFSRPSTKEATYFK
                     REAQNIDVNVDRFKEGKGLEERTRENALFLSVCAQFNGDVATGLLKWFQNLRTMQGFA
                     PMGFAPVTIDKLKAPKHKGRVLELTKVTDPGITDFDLQQKKLTLQDLPKELPDEIKKD
                     IINDGPVIVELKTTHRKVDGANKDVGEVIFDLEDNESGGTQKLVKLTGPILDTLETGK
                     VLVVDELDSRMHPLLTRLIVSLFNSPANGSNAQLIFASHDTTLLTPKLFRRDQIWFTE
                     KDQYGATDLYSLVELKGVRKEAAFGKEYIQGKYGAIPFIGDPEWLLCEGCHE"
     gene            complement(7006..7734)
                     /locus_tag="PKK20_03850"
     CDS             complement(7006..7734)
                     /locus_tag="PKK20_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNU99051.1"
                     /translation="MPSRETETIFRKPFTPEELEAFVDAAHDDDFTRPILITGICTAM
                     RRGDCCLLKWADVDLAPRFITVKTAKTGVTVAIPIFPMLYDELARHPHNAALSACGNA
                     QAGGYAFPEAAAMYEENPDGITWRVRKVFAAADFKDADEDEENGNGDAPDADAIRGEI
                     HAERKDGLRRASVRDFHSFRVTWVTLALTAGVPLELVQKVTGHKTVDVTKTPKDQMLE
                     ILDGATAKTWKKDVARLRTFVACL"
     gene            7815..7881
                     /locus_tag="PKK20_03855"
     tRNA            7815..7881
                     /locus_tag="PKK20_03855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7865..7867,aa:Ser,seq:cga)
     gene            8832..9302
                     /locus_tag="PKK20_03860"
     CDS             8832..9302
                     /locus_tag="PKK20_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99052.1"
                     /translation="MMTKLWRDALGGMFRCTAAGTPGTWTQIRPAAGDGRPVERNGSD
                     GLPDLNVTQGTLKRHAGGYSWETIVGAAGDKVGFYGATPVVHPSGANQVAVTFGNTDG
                     ENRGLTVSDLPTQAEFQALRGKCEQLADDVRALSTPVHALRGALVGTGLAKGSA"
     gene            9505..10854
                     /locus_tag="PKK20_03865"
     CDS             9505..10854
                     /locus_tag="PKK20_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNU99053.1"
                     /translation="MHFTSSTTTRLLLLLCTLSAAVRAQDFDYRIDAGAVTITRYRGS
                     GGAVNIPGTINGLPVTSIGGHAFFGCTGLTSITIPNSVTSIGPQAFYGLTGLTSVAIP
                     NSVTSIGQYAFCNCFALANVAIPNSVTTIGACAFANCALTSIALPNSVISIGNEAFSG
                     CPGLLAITVDALNLAFASQDGVMFNKSQATLIQCPGGKTGSYTIPDSVTSIGRNAFKG
                     CTGLISITIPDSVNSIGGGAFERCTGLISIAIPNSVTSIKPYAFSYCTSLTSVTIPDS
                     VTSILDGAFFGCTSLTAITIPDSVTSIGRVTGFEEPPAVDAFLGCTRLTGVYFEGDAP
                     ADYNLAFGETPATIYFLPGTTGWGSDYSRRPTAPWVLPNPVILGFGDSFGVRTNRFGF
                     IISWATNDIPVAVEACADLTQPTWLPVGSVTLKNGSAYFSDADWANHPARFYRVRSE"
     gene            complement(10861..11073)
                     /locus_tag="PKK20_03870"
     CDS             complement(10861..11073)
                     /locus_tag="PKK20_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99054.1"
                     /translation="MLAEFWRDALAGEWVCTEAGTPGTWIQIRLAAVTSDPSSGTIPV
                     GFLIWNVTMDHTKRHAGGYVWEVPGA"
BASE COUNT         2202 a         3613 c         3589 g         2305 t
ORIGIN      
        1 ggggtggagc agcccggtag ctcgtcaggc tcataacctg aaggtcgctg gttcgaatcc
       61 agcccccgca accaatttga agggccggaa catcgttcgg ccctttgttt tcaagggttt
      121 cctgcatgtc gcccattttt cgcgtcggcg ctgaaacgcg acgaacggcg tgaaatccgg
      181 tggcctaaag ccaaccaaac caccaacggg aaccaaccgc acccaccaac aagcgccacg
      241 ccgaaggcgg gcgcgagtgc ccgaatgact gcggcagtgt gatccgccgg cacgtcggcg
      301 aagatttcca gcccccccca gcccccgccc caggcgagcg cggcgcgtcc tcgcgcacgc
      361 gcgtaggtgt cgggaaactt ccagacattg gaaacctgcg ggcactcgtt ttccagacgt
      421 tggaagattc tggcgctcgc tttccaaacc ttggaaaacg gcgggccagc ggcggcggca
      481 acgttgcgga cctcgcaacg gtcgccgcct ggcgtcgtcg aaagcgtgcc ggtcaaccgc
      541 tttgacatcc tcgccagccg ggaccgtcga ccggtcccgg cgaatgagcg cagcgccccc
      601 ggcgctcagc gagagagccg ggccgggcgg tgggccgccg gcaactccag aacagggcgt
      661 gaagacggcg aagaacaggg cgaaggcggg gcgttcggca cggaaatccc gcattcaggg
      721 caaagatcga gcaggaaacg tgaagtccgg ttttggccgt taccatcacg ctgggagtgt
      781 gttgcgaagg attcggctga ataaggtgca gggaataaca ggggcggcgc tgcacgcgca
      841 ggcgcgtagc gccagcgtcc gacgcgcacg gcgtagccgg cgcgccgtcc gtcatcgtgg
      901 ggatcggact gcggcgggcg tcgcgggcgt gcgcgggatc gcactgcgcc agcagggaaa
      961 gagaagggtt ccgcttgcgg gaagtggaaa accgtaggtt gtcctttctg gcaaaggggg
     1021 accgcggggg gagatacctt cgccaaggac cggaaggccg gcgcggctga ggaacgtagg
     1081 gcgcgaagcg cgctcagtga cgaggccggg ccggacgccg ggccgccgca tggtcgcgtc
     1141 tcgccgatcg cactgcaccg ccagccacgc cagcccgcgc cggccgtcac tccttctcaa
     1201 atccctggct gtcgaacttg agagttcggc agttctcggt gacggtgcgg acaacgtggt
     1261 cgggagcgcc ggaggggatg tcggcctcga tctccaaggt cactttgacg ttggccccga
     1321 caatgccgga caggtgggca acgacctcgt ccgcgatgcg gccggcgtcg cgaccgacgc
     1381 gcttcgagtc gagcgtggcg gttccgtgga agcgtttggg cacggtagca acgggaggag
     1441 tcggcggagt tccgccgccg gtcccgccgg ttgtcccagt ggttccaccg gtcccaccgc
     1501 cggttggagt cgtcccgccg ccggttgtcg tgggcgcttc ggcagcaagc tgcttctgcg
     1561 cggcttccgg tttcacaagc aggccaatgg gactgtcgcc gtcaacccag acctggcggc
     1621 cggtctgaag accgcgatac cgcccagccg tctcgtcaaa actctcggca taggcgaagg
     1681 aatcttgcga ccaggtcaac aggcccaacc cgccgcgcac ggcttcgaca aggacgccgg
     1741 tgtcccgcaa ccggggcaga tacaggtagc gggcgaagtc ctcggtgagt tgctgaatca
     1801 ccacatgatc cccgcgccag aggggaacct gatccagttc aagccgcaac cggctggcgg
     1861 ccagcccgga aatcagcaac tcgtcgttgc ggagtttctt gctggcgcgt acagcgagag
     1921 cgtcttgtcc ggtgagccgg aacgcttgcc attcgacatc ggcttgcggg ttttgctggg
     1981 tcggcacgat gagccattgg taggtttccg gcaatcgtgc cgcgacagcg ctctgagcga
     2041 tctccttccg ggataccgcc tgcttggtct tgatagagga acgtcgccgc cgcactcaac
     2101 cggcgcagtg cgtccccgaa gatggccgac gcctcaccgg gcatgacgca accgagtttg
     2161 acccgccggt cgtctatgcc ctttgtcgcg gcctttgccg tgggtgcgga acccaggtag
     2221 atggtccgcg ccacacgccg gcaggcggaa tacttgccaa gattcggtgt ctcgccgtcc
     2281 aggcgcaacg gcagggagtt gggaccgtcc acgtcctttt cgatcaccgg cacccagttg
     2341 tcggacaggt atctggtcag ctcgaactgt acgcggggat cgtcaatcgg aatgctcgac
     2401 ggcatgatca gcggactgcg atcgcccttt tcccataggc tgtgaatcac ggacgccatc
     2461 aggcgcagaa caccgcgcgt ccgctgaaat tttacgagcg aagaccagtc ttggtagagc
     2521 cgatcaaaga cctcgggctg gatcggatag gccgccttca ggcgcttttc gtagtcgctg
     2581 tcgcggcact cgggcggaaa ctcctggtgt tgcgtgcgat acaagtcgaa aaccgccttg
     2641 gccgtagtgt cgcgagcaac aaactgctgc tcattccggc aacggcagcg caacctcgat
     2701 gagtttttta cggggttggg tcatggaatg ctgacgccgc acttggttgc gatggccacg
     2761 gcgagcttct tgaaatccgc gtgacactct tgaaccgcgt agatgtgccc tccgagcgcc
     2821 ccgtcggctg ctttgagatg aaacatgggt ttgagcgcgt cctgcgccat gggcatcagg
     2881 ctgcgaaagt gcttgagggt cgcaagacac tccggatcct ctgccgtgga acgggctgac
     2941 gtgccgcgat catggaggac ctcttggtgg tagacggcgg ggatccgctg catccatttg
     3001 tcgtatgcct tgaccggccg gtcagcgcgc acggcgtgtt gcatcacaac gtagccggca
     3061 ggtttgatat cagcggatgg cagaagaagg ttccgatcgg cggggggccg gcctaggcgg
     3121 tcgcgccatt cattcctcca atcctgaaga gtcggcccaa ggtttctcaa accttgaaga
     3181 gagaacaggt ctggtgccag cgggaacacg acgtagtttg ccgcgatgat tgcggcccgg
     3241 ttgattgcgc ccaggtttgg accgacgtca atgagcacca attccgcctc tctggcttcc
     3301 gccgctttca ggatggcgcg atgaaaggcc gattccgtcc ggaagcccgc ctcatcatgg
     3361 ttcatgcaat ctggccaagc cgacgagagt ttggcctcga agcgggacag tcccaagtcg
     3421 ccgaccagca atccaagatc acggttgatc ttctcgacat gaggctctgc gatatcgccc
     3481 aatcctcgga ggatcgggga gattgtgccg aggatgctct tcggatgatc gccgtccggc
     3541 cacaattcct ccaaccgttc ctcgctgagg aacatggcgc tcaggttggc ctgagggtcc
     3601 aagtcggccg ccacaacgga gacgccgagg tctgcgtaca tccacgcgag gtgatacacc
     3661 agcgtcgttt tcccaacgcc acctttgttg ttgaagaacg cgatcgtttt catggtttcc
     3721 tccggatcgt tgcgagcacg tgagatgtct cgaaagtgaa ctccgccgga ggatttccgt
     3781 ttcttcgcaa ggcgtcttgc gcgcgaatga cgccgcgtcc aaacatattg acgaaaccga
     3841 gcgtcgccat ggcctctgaa acaacggggt tccggtagtc ggtctgcttc gggaaatttt
     3901 cgggcgatgc catgccgtag agtccgccgg ggttctgaat ctcgatgcga tcgtcatacc
     3961 aatagaaccg aacgggggca ttcgattcgt aggagcggtg catgaccgca ttccacaata
     4021 attcgcgaat agccacagtg gggtagtcgg tctctgttcg ttcacgcagg gctgattccg
     4081 cgaccggcgc cgctgccgcc ggcagagaga gaaatgcctg catttcgcga agcaccgtca
     4141 ggagatctcc gctgaactgt ttcgtattca gcgggttgtc cgccatcgtc gtgccacccc
     4201 agcggacaaa ctggatgtaa gcgccaggaa cccaccgcaa cggttccttc gcaaacagaa
     4261 gcgcgccggc gtgtgtcgga cagtcggctc caaggtcgaa aaaacgaagt gacgccatct
     4321 gctgccggat gtctcgcttg ttttcctcga taacctcacg agccacggct gcgggcagat
     4381 aggtgactag aaacagatcg aggaccagat catcgagtgc gcagccttcg catggacgcc
     4441 catcgaaggt cttctgaaag gccgttcttt tctcggtcag gattcgctct tcctgctggt
     4501 tggcggcgcg ccgcgacggc ccgactcgaa tccagacacg ccctttgtac cgtacgggtg
     4561 gaaggttaga cggaatcacc tccacgaccg caacttcgcc atccggcaat tgcttcttct
     4621 gaacagtcaa cgccgggatt ggttccaggt tcacgttcga tcggaggttg gcgagatcgc
     4681 gaagcagcgc atcggtcacc gtcagcccgc cggctcttcc ttcatcgttg accccgacca
     4741 gcaagtagcc gggctgccga tggttcggga agtcgttggc aaaggcgcag atggcctcag
     4801 cgaatttatc ggtgttcttc gtggatatcg tccgctcgac acggtcagat tccgtgtcgg
     4861 ccatcagcgc ttgcagttgt gccgttgtca acattagcgt gctcccgttt caggtttgtg
     4921 gatgttgtct gtcgtgggcc attgtgcttc tcaggattgc agggcacgta tcgcgtcagc
     4981 agttttcgcg cattctgcat agcggtcgct tgccggtcct taagaagatc atacatcttg
     5041 gtatcgttct tctgatattt ccgcccgagc aacccggtga gtttgtcgcc gtacgcctgt
     5101 cggtggaggg cggcgtcgct gaaattgaag tgcagaaagt accatagctc aaagcactgg
     5161 tttgagtagg ccacccgtat tcggtttgtt ctcgcgagac ggaatgcttc attgaagttg
     5221 cccgcaggga agacatcccg atcgaagaca caccatactt ggttgtaggg tgtcccttct
     5281 cgcgaggcgt cgtcccgccg ggcaacggcg tccccgacca agctcaacgt gttcgcgccg
     5341 atgccctgaa catcgacttc gacgaggtca atgtccacgg agaacttgcg gaaatagttg
     5401 ggttccgtct tctcgccttc gcagacaatc aggatgcgat tgcgaatgac ccgcgtatcc
     5461 tcgcgccgcc tgtaaccctc cgcgcgttcc cagggcttac tcatggcagc cctcgcacaa
     5521 gagccactcg ggatccccga tgaacggaat cgccccgtac ttgccctgga tgtactcctt
     5581 cccgaacgct gcttccttgc gaaccccctt caattccaca agcgagtaga ggtccgtcgc
     5641 cccgtactgg tccttctccg tgaaccaaat ttgatcccgg cgaaatagct tcggcgtcag
     5701 cagcgtcgtg tcgtgcgaag cgaagatcaa ctgggcattc gacccgttcg ctggcgagtt
     5761 gaacagcgac acgatcaacc gtgtcaacag gggatgcatc cgcgaatcca actcgtccac
     5821 aacgagaacc ttgcccgtct ccagtgtatc cagaatcggc ccggtcagtt tgaccagttt
     5881 ctgagtgccc cctgattcgt tgtcttccag atcgaagatg acctcgccaa cgtccttgtt
     5941 cgcgccgtcg accttgcggt gcgtcgtctt caattccacg atcacaggtc catcgttgat
     6001 gatgtccttc ttgatctcat ccggcaattc tttcggcaag tcctggagcg tcagcttctt
     6061 ttgttgaaga tcaaagtccg tgatgccggg atcggtgacc ttggtcaact ccaagacccg
     6121 ccccttatgt ttcggcgcct tcaacttgtc aatcgtcaca ggggcaaatc ccattggcgc
     6181 aaatccctgc atcgtccgca aattctggaa ccacttgagt agtcctgtcg cgacatctcc
     6241 gttgaactgc gcgcagaccg acaggaagag cgcattctca cgcgtgcgct cttccagccc
     6301 tttcccctcc ttgaagcggt ctacgttcac gtcgatgttc tgggcttcac gcttgaagta
     6361 ggtggcttct ttcgtgctcg gtcgcgagaa gagccattca gcatgcacgc gcttccggtc
     6421 ggtttggaac ccatagcggt acagagttcc accttggagg aagaccacct cgaattgcga
     6481 aggtttgccg tcacattccg tagaaagcag gaacggctcg acgtcaatct cctcgtttac
     6541 ctgggtgtct ttggacgagg tcaaaaccat gcggcgcatc aggttcaacg ctgctagcag
     6601 gttgctcttc ccgctggcat tcgcaccgta cacaaccgca ctgcgcacca gacggtgttt
     6661 ctccgtggca atgacgttcg actcttccag ggtgtcgtca gtcgaggcca ccatgctgaa
     6721 agtcgcccgg ttcttgaagg aaaggaagtt cgtcacattg aattctataa gcatagatga
     6781 ctcccacagt tcaagaatgc ctgaatatcc gtttaggacg ataatgaaca tgcgtctttc
     6841 gcggaaaaga tgcaataaga aaaagaatag ctccgacaac gcctattatc agcgcgtctt
     6901 ttcctgatgc ctcccataag cttcgagcgg gcgacgaaga ctggcgcgcc tgcctgcgtc
     6961 gggcctacgg cgggcaggtc acagcgccgt ctgtgtctgc ctccgtcaca ggcaggcgac
     7021 aaatgtccgg agtcgggcga cgtctttctt ccacgtcttg gccgtggccc cgtcgaggat
     7081 ctcaagcatc tggtctttcg gcgtcttggt aacgtcaacc gttttgtgcc ccgtgacctt
     7141 ctgcacgagt tcaagcggca cgccggcagt cagcgccagt gtcacccatg taacgcggaa
     7201 gctgtggaag tcccgcacgc tggcgcggcg cagtccgtcc ttgcgttcgg cgtgaatctc
     7261 gccgcggatg gcgtccgcgt cgggcgcgtc gccgttcccg ttctcctcgt cctcgtcggc
     7321 gtccttgaag tcggcggcgg cgaacacctt gcggacccgc caggtgattc cgtcggggtt
     7381 ctcctcgtac atggccgccg cttccgggaa ggcgtagccg cctgcctgtg cgttgccgca
     7441 cgcagacagg gcggcgttgt gcgggtgccg ggcgagttcg tcgtagagca tggggaagat
     7501 cgggattgcc accgtcacgc cggtcttggc cgtcttcacg gtgatgaacc gcggggcaag
     7561 gtcaacgtcg gcccacttca acaggcagca gtcgccccgg cgcatggccg tacagatacc
     7621 ggtgatgagg attggccggg tgaagtcgtc gtcgtgcgcc gcgtccacga aggcctcgag
     7681 ttcctccggg gtgaacggct tgcggaagat cgtctcggtt tcgcgtgagg gcacgccgga
     7741 gaagggattg atgctccacg ccgaggttgt ccgccaaccg gaatcagctt gaagccgtga
     7801 atatcgagga aaaggcggga atatccggtt gcgcttcagg ctcataacct gaaggtcgct
     7861 ggttcgaatc cagcccccgc aaccaattta cgttgcgccg caacgactta agccgctggc
     7921 cttcagcggc ttttcttcat tcttggcacg cttcttgaat ccgcgcctct ccgtgcacca
     7981 ctgtgcacta ctgtgcaccg ctctgcacca ttaccaaaca tttgccaaac attttggcgg
     8041 tcgggagggg gcgggaggag cgcaggttcg atgttcgggc acaggcacgc cgggccagtc
     8101 atgtccgatt gccaacccag gcgcaatccc ggtgtggcct gcagcgcatc gatctcagtc
     8161 atactgtgct tcggtctttc ccgcgtaaac agcctgaaag aacgcctgaa tctgactcgg
     8221 cagaaccctg ctcaacgaca gctccggcag tctgtccgcg gcaaagaagg cggactccga
     8281 tgactcggat gtcttcctcg caaccccgcc ggtaatctcg caatggaaga acatactgcc
     8341 tcaacgccca ctgctgctct ctcgacaggt tcgtgatgag aagtgctgca cagtcagtag
     8401 gaacagcgtc agtgcgtcgt cattgtgcgc cgtaaccctt ctcgaccacc gccgcccgga
     8461 tttcactaag tagcgtaata gcggtgacca agccgtcatt gatcgcctgg acctgagcct
     8521 gcgtcggcgg gtcgctgacg gtcctggacg ggcagtctga tctccgctca ggccgggtcg
     8581 gacgtcggat tggccgacca cttggccgcg acatcggtga tggcgaggtc acttgaatcg
     8641 tctggcggca tactcgctat ctcagccctc ccgtcgatca ggccccgcaa accggcctgg
     8701 cacggatgtc taggccggta gaagacggtc agtccgacgc tgggccaagg ctgtgaatcg
     8761 gccagcgcgt taccacgtac cagaacggcg cgcccaccac gaccatcggc ccgccggtaa
     8821 gcgggacgca tgtgatgacc aagctctggc gcgatgccct gggcgggatg ttccggtgca
     8881 cggcggcggg cacgccgggg acgtggaccc agatcaggcc ggcggctggt gacggcagac
     8941 ccgtcgagcg gaacggttcc gacgggctac ctgatcttaa cgtgacgcag gggacgctga
     9001 agcgccatgc cgggggatac tcgtgggaga caatcgtcgg cgcggccggg gacaaggtcg
     9061 ggttctacgg tgcgacaccg gtagtgcacc cttcaggggc caaccaggtg gcggttacgt
     9121 tcgggaacac ggacggtgag aacagagggc tcaccgtttc cgatctgccc acccaggccg
     9181 agtttcaggc cctgcggggc aagtgcgagc aactggccga tgacgtccgg gcgctgtcga
     9241 ccccggtgca tgccctgagg ggggcgttgg tcggaaccgg cttggctaaa ggaagtgcgt
     9301 aagcgagcgg catgtcagct tggcggaatg ggtcgcagcg actgggtcat actgggttca
     9361 tgtgcagcga aattggcgaa tcgtctcgat ctgccaaatt ggctcttgct aattcgatac
     9421 aaataggaga gaggttatcg tactaagtgg atttccgcga tgaaacgctc ttttgcagaa
     9481 acaacgcgct cgaagaggat ggccgtgcat ttcacatcct ccaccacgac gcgactgctg
     9541 ctgctgctct gcacgctgtc cgcggcggtg cgggcccagg attttgacta caggatcgat
     9601 gctggggcgg tcaccatcac ccgctacagg gggtcgggag gcgcggtgaa catccctggc
     9661 acgatcaacg gcctgccggt gaccagcatc ggggggcatg cgttcttcgg ctgcactggg
     9721 ctgaccagca tcacgatccc caacagcgtc accagcatcg ggccgcaagc gttctatggc
     9781 ctcaccggcc tgaccagcgt cgcgatcccc aacagcgtca ccagcatagg gcagtatgca
     9841 ttctgtaact gcttcgctct ggccaacgtc gcaatcccca acagcgttac caccattggg
     9901 gcgtgcgcat tcgcgaattg cgccctgacc agcatcgcgc tccctaacag cgtgatcagc
     9961 atcggcaacg aggcgttctc aggctgtccc ggcctcttgg cgataaccgt agatgcgctc
    10021 aacctcgcct ttgccagtca ggacggggtt atgttcaaca agagccaggc gacactgatc
    10081 caatgtccgg gcggcaaaac cggaagctac acgatccccg atagcgtcac cagcatcggc
    10141 cggaatgctt tcaagggctg caccggactg atcagcatca cgatccctga cagtgtcaac
    10201 agcatcgggg ggggcgcctt cgaacgttgc accggcctga tcagcatcgc gatccccaac
    10261 agcgtcacca gcatcaagcc gtacgccttc tcgtattgca ccagcctgac cagcgtcaca
    10321 atccccgaca gcgtcaccag tatcttggac ggggcgttct tcggctgcac cagcttgacc
    10381 gccatcacga tccccgacag cgtcaccagc atcggccgag tcaccggctt cgaggagccc
    10441 ccggctgtgg atgcgttcct cggctgcacg cggctgacgg gcgtctattt cgagggcgac
    10501 gctccggcag actacaactt agcatttggg gaaacccccg cgacgatcta cttcctgccc
    10561 ggaaccactg ggtggggatc ggattacagc cgtcgcccga ccgccccttg ggtgctgccg
    10621 aaccccgtga tcctgggctt tggcgacagc tttggtgtgc gaaccaatcg gttcgggttc
    10681 atcatttctt gggcaacaaa tgacatcccg gtggccgtgg aagcttgcgc ggacctgaca
    10741 caaccaacct ggctgccggt gggcagcgtg accctcaaga acggctccgc ctacttcagc
    10801 gacgccgact gggcgaacca ccctgctcgg ttctaccgcg tgcggtcgga gtgacgcgca
    10861 ctaagccccc ggcacctccc acacgtaccc gccggcgtgg cgcttggtgt ggtccatggt
    10921 gacgttccag atcaggaacc cgaccgggat ggtgccgctt gacggatcgg acgtcaccgc
    10981 cgccagcctg atctggatcc acgtccccgg cgtgcccgcc tccgtgcaga cccactcacc
    11041 agcgagcgcg tcgcgccaga actcggccag cacatgcgtc ccgctggtcg gcgggccgat
    11101 caccgtcgtc gggctggcgg tctggtacgt ggccaggcgc agggcggtct gcggctccta
    11161 ccgctgtcag actgatcgac cttctgcctg tggaaacgct cgtggcgaag ccttctggac
    11221 cgtccgatcc gagcttcggt gtcgtcaccc tcctggctga tcagatctcg ggaaacgggc
    11281 tgccagaggg ctgcattcgg caaggaggac cggtctgcga ggctctggcc agccgttcgc
    11341 ggccagactg accgtgtccg ccggttgggg tgtcaacgag atccgggtca ggcgagggcg
    11401 agttcaagtc acctgcgaga gcggtctgag aagcccgcct cccataagca aggatagaac
    11461 gccgccagcg gagcgttccg gcagcgcctt gccggggcct cgacgcgagg agtcccgctc
    11521 cgagcgcaga tcgggtctcg gccaatgggg attgcgcgcc cgctcgaccc cctaccacgc
    11581 tgcgcagcgc ctcagcgctc cgctacccga cttctccgtt agctcaggtc aaggccccgc
    11641 ctcaaggaac tgcaactagc agcctgtcgg acttagatcg agaagcaagt gagggtgatg
    11701 gtggtagga
//
LOCUS       DAOQRO010000065        11689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4668_length_11689_cov_4.752965, whole genome shotgun sequence.
ACCESSION   DAOQRO010000065 DAOQRO010000000
VERSION     DAOQRO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4668_length_11689_cov_4.752965"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKK20_03875"
     CDS             <1..391
                     /locus_tag="PKK20_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNU99055.1"
                     /translation="AFFSARQIASRNRLHFLRMDGKETYKNAVQAMLSAAQEVLRRCE
                     LDVSQIKCIIPHQANRRIIDAVGARLGARPDQLFVNLEKYGNTSAASVGIALDEAVSL
                     GRIERGDLVLLTVFGGGFTWAAALIEW"
     gene            480..806
                     /locus_tag="PKK20_03880"
     CDS             480..806
                     /locus_tag="PKK20_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99056.1"
                     /translation="MSTSKLDTSDVLRPFPAASSPTRLLLPAEQVAIRKNGIEFLSPN
                     PIPLWTEVTVDLRSAQGERPVRGCGVVVDCMGDRRTGYIVSLMMTSLTRQSQERLCQL
                     AADRLV"
     gene            832..1920
                     /locus_tag="PKK20_03885"
     CDS             832..1920
                     /locus_tag="PKK20_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNU99057.1"
                     /translation="MELFIRILQTAINGGASDVHIKPGGPVIFRIERSLVAIEAPTPT
                     EEWVNKIVETIVPRHATARLEEHREVDFSYFVPNVGRFRTNVFQQKGQWCLAMRYVKI
                     KVPSFEELGLLPILKSIAESPRGIVLLSGSTGCGKSTTLAAMVEHINATSKKHIITLE
                     DPIEYVFEDNQSVIEQREIGLDSLSFEMGLKHILRQDPDVIMIGEMRDAVSFMAAINA
                     ADTGHLVLSTLHTTTASQAIGRILDFFQPDERDQVRRQLAATMQAIVCQRMAARVGGG
                     VVPAIEIMINTPTVRKMLEENRLEKLAAAIETGVEDGMQTFNQALFNLVREGRVSEKE
                     ALAKATNPQALEMNFKGIFLDEARRILT"
     gene            complement(2045..3457)
                     /locus_tag="PKK20_03890"
     CDS             complement(2045..3457)
                     /locus_tag="PKK20_03890"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNU99058.1"
                     /translation="MREEEPRQPPPERLLQLIWMHQRLRRDDVHTTDGRPVVVLHPGF
                     WNREAGPDFREAVIQIGEPPARVGDVEVDLCSSGWSQHQHQVNPAYRKVILHVVWESP
                     GAASGALPTMAVREWIDAPLAELECWLGGLNAAELPPALRGACVDPLSRLAAGGFEEL
                     LRQAARVRLEQKAARFQARARHAGWEQALWEGLFGALGYKHNVWPLRRLAELLPRFRA
                     CIDGRPPSARRWQVLLLGVAGLLPDALARPSRGADAFLISLWNEWWRERARFAEDLIP
                     RGLWRFHGIRPANHPARRLALAAHWLDDPTWLDRLEGWLAEDIPDRRLPATLLERWQV
                     APHDYWSWHWTLRSGHLARPRPWLGLQRVTDLAMNVVLPWLWSRAGTGGDPALLGRAE
                     HRYFAWPAAQDNAVLRLARQRLLAGRSLPKPRRAADQQGLLQIVRDFCDHADALCQSC
                     PFPELVNATALSGGQPGQVD"
     gene            3659..4867
                     /gene="lpxK"
                     /locus_tag="PKK20_03895"
     CDS             3659..4867
                     /gene="lpxK"
                     /locus_tag="PKK20_03895"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNU99059.1"
                     /translation="MRQTVRAWTEAAETFALEVIFEQRQGKRATFLRGLLFGLSKLFA
                     LAVKARRFLYDVRILRDSTIGVQVIAIGNLTVGGTGKTPVVEKFARELKDQGRNVAIL
                     SRGYRSKPTPLARRLLNKALFREEKTPPRVVSDGKSLLLDSDTAGDEPYMLASNLKDV
                     VVLVDKDRVKAGLYAIEKFGCDTLLLDDGFQYWKLRGRRHDVVLVDRQQPFGNEHLLP
                     RGTLREPPSHLARARTVFITKSDGQTTDLRRRISQYNRDARVVECVHQPLYFEDVFTG
                     QRRGLDLLRDRRIASLSGIAQPESFESSLIQLGARLVYTKRFADHHRFTQQEVLNVIN
                     RSKKRQASMVITTQKDAVRFPKLDRRDLPVYFMRVEIRIVRGAKDFQECVRQICFRQS
                     EDRSGSKQRH"
     gene            4901..6379
                     /locus_tag="PKK20_03900"
     CDS             4901..6379
                     /locus_tag="PKK20_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HNU99060.1"
                     /translation="MIITSLSRLPVASRRTAFLHFLRGGAATLLLIGLVAGCAGAKPV
                     PASAGHSRALETPLDRYVAGPDPAYAWQAVTNARINDVTVTIIDMTSQSWLTTNEVDR
                     PLWRHWLVVARPDALEHSTALLMIGGGNNKDKGLPKPSDALIQMARQTRSMTAELRMV
                     PNQPLVFGQDGRERVEDDLVAYTWDKFLRTGDDRWPARLPMTKAAVRAMDTVSAFCAS
                     TAGGGAKVDTYVVAGGSKRGWTAWTTAAVDKRVVAVCPIVIDLLNIVPSFEHHYRTYG
                     FFAPAIKDYVGAGIMDWMNTPEFKRLMRIVEPFEYRDRLTMPKLIVNASGDQFFLPDS
                     SRFYFDRLPGVKYLRCVPNADHSLKNSDAWETLMAWHHATLNRTHLPRFSWQHEGGDA
                     IRVRTVDQPTDVRLWQATNPAARDFRLDVIGPAWTNQVLRQDDRGAYLGRVTSPSQGY
                     TAFFVELTYPLGGPAPLKLTTDVRVTPDTLPHPPYQPKPLKR"
     gene            complement(6392..8269)
                     /locus_tag="PKK20_03905"
     CDS             complement(6392..8269)
                     /locus_tag="PKK20_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNU99061.1"
                     /translation="METAPAQSDPPRPDRQRLDVDIVCVGFGPATAGFLTTLSKQLVN
                     SDGSPAIESAVMPGLPLQVMCYERADDIGFGVSGVVTRGHGLRTTFPDLDRANIPMSA
                     PVTHEKVLYLLDPVGASRRSAVLRGADRMLRLFQGVLPLRHQALELPWTPSFLHKRGG
                     LVLGLGQFLQWVGAQVQSGGAIQIWPGTPVAQALIENDKVVGVRLIDQGVDRLGQPSD
                     GFLPGMDIGAALTVVGDGPVGAIGQQLDAHFGLPPGHYIRDWAAGMKFVVDLPEGTPL
                     EAGTVLHTFGYPEPEIFGFFYVHPNRVASLGIFVPSWFDSPMRTAYDYLQHWMRHPYL
                     WRFLDGGKLRSWGAKTLGESGRRGEPHLVGDGYVRIGEGSGSTNVLTGSGVDEAWVTG
                     TLLAEAVLELLKARQPFTRANLEAAYVRRRRSSWVEREALIAEKSRDGFHKGVVRGMI
                     GMALAGLTNGRFTLTGNPVPPWKRIPSVEAYYRGRVTPSEIERLREECQAKGVSLHAA
                     LMEKVGWPSIPRDGQLLVSHQDALLMGGKVQAPSGYADHVVFLYPELCQRCGTKICIE
                     ACSGQAITLGEGGVPVFDREKCVHCGVCYWNCATPLPQQPDRTNIAFRAGSGGLHSAE
                     N"
     gene            8408..>11689
                     /locus_tag="PKK20_03910"
     CDS             8408..>11689
                     /locus_tag="PKK20_03910"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNU99062.1"
                     /translation="MKVSFHAQWAVRSLLAAGLLTMGVGHGQVLLSEFQASNLRTLDD
                     EDGNSADWIEIHNAGDLPVDLAGWSLTDDAGQLRKWQFPSTNIAPGQYLVVFASGKNR
                     RIPGRPLHTDFRLSASGEFLALVKADGRTLATAYSPAYPPQAEDISYGLPLRPTSHAL
                     VGPRAPGKLLIPRDDDLGTNWVRPDFDDSDWTPVVTGVGFNVPGDLVGSDIEGAMRGI
                     NPTAYLRIPFVVTNLAALDRLTLNIRYNDGFAAYLNGFPIAQRNAPVTAPGGVLANSA
                     ADWSFTGQQGFNNWYYGFYDLTADADGRYDAGRDFVSSHPQWSWNGGAWVLGPANPPW
                     DAITQGGWFSNGENSGGVHWVIRRWVCEAPGTFVCQVAFAKENPTCGNGATLRVFVNG
                     NEQYQATVAFDDTTGLHTNLTVTDLAAGDTLDFALDPLGTDGQRTDHCDEVTFSVVID
                     QIPSDGAVWNTMANAARTPDETLVPESFDLTRYRDVLISGTNVLAIQGLNISADDADF
                     LLAPELAGGCLGLSLDEHVYFPKPTPGEANGSGTAALGPIISQVEHQPVVPEDSEDLI
                     VRARVTSSFGPVGAVTLRYRVMYSGEASIPMRDDGVHEDGDAGDGIYAARIPAAAAGP
                     GQMIRYAILAADDQGRTSRLPAYTDPLGSPQYRGTVVRDPALEASRLPVLHWFIQNPS
                     AANSDTRASGSLFFDGEFYDNVGATIHGQSTRGFPKRSYDLDFNPGYKFRWSDDAPRV
                     DDLNLLTTWADKSHMRNVLAHETYRDAGAQSHFAFAVRVQQNGAFFSVANLVENGDDN
                     FLKRLGLDPRGALYKMYNSADSAAGNEKKTRKNEGTADLAALIQGMSQAGAKAREAFM
                     YDNLDVPQMINFLAAKIITADTDCCHKNYYLYRDSEGTREWMAMPWDVDLSFGRVWTG
                     AHAYYDEQIYTNQSIFIGSGNIVFSPIYNTSATRQMFLRRLRTLMDDLLQPLATPATN
                     DSYRIKSRALRDQIAPDAALDLAKWGTWGTRETITQAVDRIWNQFLPGRRVYLFRTLS
                     VTNGGEIPLPQPADAVVEIGSLEYRPASGNPAEEWLSITNANPYAVDLSGWQLDGAVR
                     FTFKPG"
BASE COUNT         2197 a         3777 c         3631 g         2084 t
ORIGIN      
        1 cgcgttcttt tccgcgaggc aaatcgcctc gcgcaaccgg ctgcattttc ttcggatgga
       61 cggcaaagag acgtacaaga atgcggtgca agccatgctg tcggcggccc aggaggtgtt
      121 gcggcggtgc gaattggatg tttcgcaaat caagtgcatt atcccccatc aggcgaaccg
      181 gcgcatcatc gacgctgtcg gggcgcgatt gggagccaga cccgaccaac tgtttgtcaa
      241 tctcgagaag tacggaaaca cgtcggccgc ctccgtgggg attgccctgg acgaagcggt
      301 gtccctgggg cgcatcgaac gcggcgatct tgtgctcctg accgtcttcg gcggcggctt
      361 cacgtgggct gcggccctga tcgaatggtg aatcgggttc ggccatgccg catcccgccg
      421 ccgggcgtgc ggccggcgtt gacgaaaaga agtattgtgg taggctgcat tcaacatcta
      481 tgagcaccag taaactggac acttccgatg tcctccgtcc attccccgcc gcctcatccc
      541 caacacgatt gcttctcccc gccgagcagg ttgcgattcg caagaacggc atcgagtttc
      601 tgtcgcccaa tccgatcccg ctctggacgg aggtgacggt cgatctgcgc tctgcgcagg
      661 gagagcgtcc agtgcgcggt tgcggcgtgg ttgtggattg catgggggac aggcgcaccg
      721 ggtatatcgt ctcgctcatg atgaccagcc tcacgcgaca gtcgcaggaa cgcctttgcc
      781 agcttgccgc ggatcgactg gtctgattcc tgctacttcc aacacccaca catggaactc
      841 ttcattcgga tcctgcaaac ggcgatcaat ggcggggcgt cggacgtgca tatcaagccg
      901 ggtgggcccg tcatcttccg gattgagcgt tcactcgtcg ccatcgaggc ccccacgccg
      961 acggaggaat gggtgaacaa gatcgtcgag accatcgtgc cgcggcatgc caccgcgcgg
     1021 ctcgaagaac accgcgaggt cgatttctcc tacttcgttc cgaatgtcgg ccggtttcga
     1081 accaacgtgt ttcagcagaa gggccagtgg tgtctggcga tgcgctacgt caagatcaag
     1141 gtgcccagct tcgaggaact gggcctgttg ccgatcctga aatcgattgc cgaatcgcca
     1201 cgcggcatcg tgttgctgtc cggatccacc ggatgcggca agtcgacgac cctggcggcg
     1261 atggtcgagc acatcaatgc cacctcgaag aagcacatca tcactctgga agaccccatc
     1321 gagtacgttt tcgaggacaa ccagtccgtg atcgaacagc gcgagatcgg tctcgattcg
     1381 ctctcgttcg agatgggtct gaaacacatt ctgcgacagg accccgacgt catcatgatc
     1441 ggtgaaatgc gcgatgcggt gagtttcatg gccgcaatca atgccgcgga caccggccac
     1501 ctggtcctct cgacgcttca cacaaccacc gcgtcgcagg ccattggacg catcctggat
     1561 ttcttccaac cggacgagcg cgaccaggtc cgccgtcagt tggcagccac catgcaggcc
     1621 atcgtctgcc agcggatggc agcacgggtt ggcgggggcg ttgtgcccgc catcgagatc
     1681 atgatcaaca cgccgacggt tcgcaagatg ctcgaggaga accggctcga gaagctggcc
     1741 gcggcgatcg agaccggcgt cgaggacggc atgcagacgt tcaaccaggc cctcttcaac
     1801 ctggtcaggg agggaagagt gagcgagaag gaagcccttg ccaaagccac caatccgcag
     1861 gcgctcgaaa tgaatttcaa aggcattttt ctcgacgaag cacggcgcat tctcacctga
     1921 cctccccgcg tgttcccggc tacgaggacg agcgcctcgc gttgcggaga aggaccgtcg
     1981 tcgacgcgcc cgctcgtggc gcgggttgtt cgcgactggc caacgggagt cggcgccggg
     2041 aagatcagtc aacctggcca ggctgcccac ccgagagggc tgttgcattc acaagctcag
     2101 ggaacggaca gctctggcag agggcgtcgg cgtgatcgca gaaatcccgg acgatctgaa
     2161 gcaacccctg ctggtcagcc gcccgccgcg gtttggggag cgagcggcct gccagcagcc
     2221 tctgccgcgc gaggcgcagc acggcgttgt cctgcgccgc gggccaggcg aagtaacgat
     2281 gttctgcgcg ccccagcagt gccgggtctc cgcctgtccc cgcacgactc cacagccacg
     2341 gcagcacgac attcatcgcc aggtcggtga cgcgctgcag gcccagccag gggcggggtc
     2401 tggccaagtg acccgaccgg agtgtccagt gccacgacca gtaatcatgg ggcgcaacct
     2461 gccagcgctc gaggagggtg gcaggcaagc gacggtccgg gatatcctcc gccagccagc
     2521 cttcgagccg atcgagccat gtcggatcgt ccagccagtg tgcggcgagc gccaaccgcc
     2581 gtgccgggtg gttggcggga cgaatcccat gaaaccgcca caagcccctc gggatcaggt
     2641 cctcggcaaa tcgcgctcgc tcgcgccacc attcgttcca aagactgatg aggaatgcat
     2701 cagcgccccg ggaaggacga gccaaagcgt ccggaagcag accggcgact ccgagcagga
     2761 ggacctgcca gcgacgggcg gaggggggcc ggccatcgat gcaggcgcgg aatcggggca
     2821 gtaattcagc cagtcggcgg aggggccaga cattgtgttt gtagccaaga gctccaaaga
     2881 gcccttccca gagcgcttgt tcccaacccg cgtggcgggc gcgggcttgg aaccgtgctg
     2941 ctttttgctc gagacgcacc ctggcggcct gccgcaacaa ctcttcgaag ccccctgcag
     3001 ccagccggga gagcgggtcg acgcacgcgc cgcgcaacgc cgggggcagt tcggcggcat
     3061 tcaatcctcc cagccagcat tcgagttccg ccagcggtgc atcgatccac tctcgcacgg
     3121 ccattgtcgg caaggcacca gaggcggctc ccggcgattc ccacacaacg tgaaggatga
     3181 ccttccggta ggccgggttc acctggtgct gatgttgcga ccagccgctc gaacagagat
     3241 ccacctcgac gtccccgact cgagccggcg gctcgccaat ctgaatcacc gcctcgcgga
     3301 aatcggggcc ggcctcgcgg ttccagaagc ccggatgcag cacaacgacc ggtcgaccgt
     3361 cggtcgtgtg cacgtcgtct cgacgcaacc gttggtgcat ccagatcaac tgcaacaggc
     3421 gttcgggcgg cggctgccgg ggctcctcct cgcgcacttg ggcgggaacc gatcgcgcct
     3481 tccgccattt ctggtattga ttttcaggga atcctgccac ctcggcgttc tatgtcgtcc
     3541 gggattggcc tgatgtcaag ggggcaaagt gccgggcggc atcacgcgat gcgcattgtc
     3601 gtgtctcgtt cgggccccgg cttgctcttg ccgaggcggc ttggcatggt agtcgcccat
     3661 gcggcaaacg gtgcgagcgt ggacggaggc ggccgagact ttcgccttgg aggtgatctt
     3721 cgagcagcga cagggcaagc gcgctacgtt cctgcgcgga ttgcttttcg ggctctcgaa
     3781 gctgtttgcc ttggccgtca aggcgcggcg gttcttatat gatgtccgca tcctgcgcga
     3841 ttcgaccatt ggcgtgcagg tgatcgcgat tggcaatctg accgtgggcg gcaccggcaa
     3901 gacgccggtc gtcgagaagt tcgccaggga gctcaaagac cagggacgga acgtcgccat
     3961 tctgtcgcgt ggttaccgat ccaaaccgac gcccctggca agacgccttc tgaacaaggc
     4021 gctcttcagg gaagagaaga cgccgccccg tgtggtctcc gacggcaagt cgctgttgct
     4081 cgattcggac acagccggcg acgaaccgta catgcttgca tccaatctga aggacgtcgt
     4141 ggtgctcgtc gacaaggacc gcgtcaaggc cggcctctac gccatcgaga agttcggctg
     4201 tgacacgctg ctcttggacg acggatttca gtactggaag ctgcgcggac ggcggcacga
     4261 tgtggtgctg gtggaccgcc agcagccctt tggcaacgaa cacctgcttc cgcgaggcac
     4321 gctgcgcgag ccgccctccc acctggcccg cgcccgcacg gtcttcatca cgaagagcga
     4381 cgggcagacc accgacctgc gccgtcggat cagccaatac aatcgcgacg cccgggtggt
     4441 cgagtgtgtc caccaaccgc tgtatttcga ggatgtcttc accggccagc ggcggggact
     4501 ggacttgctc agggaccggc ggattgcctc gctcagcggc attgcccagc ctgagagctt
     4561 cgagtcgagc ctgatccagc tcggcgccag gctggtctac accaagcgat ttgcggatca
     4621 tcaccgcttt acgcagcagg aagtgctcaa tgtcattaac cgcagcaaga agcgccaggc
     4681 ctcgatggtc atcaccacac agaaagacgc cgtccgtttc ccaaaactcg accggcgcga
     4741 cctccccgtc tatttcatgc gtgtcgagat cagaattgtg cggggcgcca aggactttca
     4801 agaatgcgtg cgccagattt gttttcggca gagcgaagac cgttccggct caaaacagcg
     4861 acattgacag attcgaacgg ctcgctttcc tagcggcagc atgatcatca cctcactgtc
     4921 gcgtctgccg gttgcctcgc gccgcacggc ttttctccat ttcctgcgcg ggggcgccgc
     4981 caccctcttg ctgatcggcc tggttgccgg gtgcgccggc gccaaaccgg tcccggcttc
     5041 ggccggccac agccgagccc tcgaaacgcc gctggatcgc tacgtcgccg ggcccgatcc
     5101 ggcctatgcg tggcaggcgg tcacaaacgc ccggatcaat gacgtcacag ttacgatcat
     5161 cgatatgacc tcccaatcgt ggctgacgac caacgaggtc gatcgccccc tttggcgcca
     5221 ttggctggtc gttgcgcggc ccgatgctct cgaacactcg actgctctgc tgatgatcgg
     5281 cggcggcaac aacaaggaca aaggactgcc caaaccgtcc gacgcactga tccagatggc
     5341 acggcaaacc cgctcgatga ccgccgaact gcgcatggtg cccaaccaac cgctcgtctt
     5401 cggacaggac ggacgcgagc gggtcgagga tgatctggtc gcctatacat gggacaagtt
     5461 cctgcggacc ggagacgacc ggtggccggc ccggctgccg atgaccaaag cggccgtgcg
     5521 tgcgatggac accgtgagcg cattctgcgc cagcacggcc ggaggcggcg cgaaggtgga
     5581 cacgtacgtg gtggccgggg ggtcgaagcg cggatggacg gcgtggacaa cagcggccgt
     5641 ggacaaacgg gtggtggcgg tctgccccat cgtgatcgac ctgctcaata tcgtgccgtc
     5701 gttcgagcac cattaccgca catacggctt cttcgccccg gccatcaagg actatgtcgg
     5761 cgcgggcatc atggactgga tgaacacacc cgaattcaag cggctcatgc ggattgtcga
     5821 gccgttcgag tatcgggatc gcctcacaat gcccaagctg atcgtcaacg cgagcgggga
     5881 ccagttcttc ctgcctgact cgtcgcggtt ctactttgac cggctgccgg gcgtgaaata
     5941 tctgcggtgc gtcccgaacg ccgatcactc gctcaagaac agcgatgcat gggagacgct
     6001 catggcctgg caccacgcaa cgctcaaccg cacacatctt ccgcgcttct cctggcaaca
     6061 cgagggcggg gacgcaatcc gggttcggac cgtcgatcaa ccgaccgatg tccggctctg
     6121 gcaggccacg aatcccgcgg cgcgcgattt caggctcgat gtcatcggcc ccgcctggac
     6181 gaaccaggtg ctgaggcagg acgaccgcgg ggcctacctc gggcgcgtca cctctcctag
     6241 ccaaggctat acggccttct tcgtcgagtt gacgtatcca ctgggcggac cggctcccct
     6301 caaactcacg acggacgtcc gggtcactcc cgacacactc ccccacccgc cgtatcagcc
     6361 gaagccgctg aaacgttgag ccgcgccccg ctcaattctc ggcggagtgg agacctccgg
     6421 agccggcacg gaaagcgatg ttggtgcggt caggctgctg cggaaggggc gtggcgcaat
     6481 tccagtagca gacgccgcag tgaacacact tctcacggtc gaacacgggg actccgcctt
     6541 cgccgagcgt gatcgcctgg ccggaacagg cctcgatgca aatcttcgtg ccgcagcgct
     6601 ggcacagttc cgggtagaga aatacgacgt gatccgcata gcccgagggg gcctgcactt
     6661 tgccccccat gagcagggca tcctgatggg acaccagcag ttggccatcg cgcggaatgg
     6721 aaggccagcc gactttctcc atcaaagccg cgtgcagtga aacgcccttg gcctggcatt
     6781 cctcacgcag gcgctcgatc tccgatggcg tgacacgtcc gcggtaatac gcttcgacag
     6841 agggaatccg tttccaggga ggcacggggt tgcctgtcag ggtgaatcgc ccgtttgtca
     6901 gcccggccag cgccatgccg atcatgccgc ggacgacgcc tttgtggaaa ccgtcacgcg
     6961 atttctcggc gatcagggcc tcacgctcga cccaactcga acggcggcgt ctgacatagg
     7021 cggcttccag gttggccctg gtgaagggct gccgggcctt cagcagttcg agcaccgctt
     7081 cggccagcag cgtccccgtc acccacgcct catccacgcc ggagccagtg aggacattgg
     7141 tgctgccgct gccttcgccg atgcgcacgt atccgtcgcc caccagatgc ggttcgccgc
     7201 gtcgtccgga ttcgcccaac gtcttggcgc cccaggagcg gagtttgcct ccgtcgagga
     7261 agcgccagag atacggatgc ctcatccagt gctgcaaata gtcgtaggcg gtgcgcatcg
     7321 ggctgtcgaa ccacgacggc acaaagatgc cgagtgacgc cacccgattc ggatgcacat
     7381 agaagaaacc gaatatctcc ggctcgggat agccgaaggt gtgcaagacg gtgccggctt
     7441 cgagcggcgt cccttccggc aagtcgacca cgaacttcat gcccgccgcc caatcgcgga
     7501 tgtagtggcc cggcggcaga ccgaaatggg cgtcgagctg ctggccgatt gcccccaccg
     7561 ggccatcgcc gacaactgtc agcgccgccc cgatgtccat ccctggcaga aaaccgtcgc
     7621 tgggctgtcc gagtctgtcg accccttgat cgatgaggcg gactccgacg accttgtcat
     7681 tctcgatcag ggcttgggcg acgggtgtcc ccggccagat ctgaatcgcg ccgccgctct
     7741 gcacctgcgc gccgacccac tgcaagaact gacccaaccc gagcacgaga ccgccgcgtt
     7801 tgtggaggaa cgacggtgtc caaggcaatt cgagggcctg atgtctcaac ggcaaaacac
     7861 cttggaacaa acgcagcatc ctgtccgcac cccgcaggac tgccgaccgc cggctcgcgc
     7921 cgaccggatc gagcagatac aggacctttt catgggtgac cggcgcgctc attgggatgt
     7981 tggcccggtc aagatccgga aaagtcgtcc gcaggccatg cccgcgggtc accacgccgg
     8041 agacgccgaa gccgatgtca tcggcgcgct cgtagcacat gacctgaagg ggcaaaccgg
     8101 gcatgaccgc gctttcaatg gccggactgc catcggaatt gacgagctgc ttcgagagcg
     8161 ttgtcagaaa cccggctgtt gccggaccga acccgacgca gacgatgtcc acatcgagtc
     8221 gctggcgatc cggacggggc ggatcggatt gagcgggtgc tgtttccacg acactggttc
     8281 cggccttcgg cagccggcaa gagaccctaa gtggaaaccg ggatgcgggt caattgcccg
     8341 gttcttgctg tccctgggcg gcttgggtgg tatgattctt gccagtcagt caatcgatga
     8401 acagaccatg aaagtctcat tccacgcgca gtgggccgtt cgatcgttgc tggccgctgg
     8461 tctcctcaca atgggggtcg gccatggcca ggttctgctg tccgaatttc aggccagcaa
     8521 cctgcgcacg ctcgacgacg aggatggcaa ctctgcagac tggatcgaaa tccataacgc
     8581 gggcgatctc ccggtcgacc tggcaggatg gtcgctgacc gacgatgccg gccagctcag
     8641 gaagtggcag tttccctcga ccaacattgc ccctggccag tatctcgtcg tcttcgcgtc
     8701 aggaaagaac cgccgtatcc ccgggcgtcc gcttcacacc gatttccgtt tgagcgcgtc
     8761 gggtgaattc ctggcattgg tcaaagctga cggacgcact ctcgccacgg cttacagtcc
     8821 cgcgtatccc ccacaggctg aagacatcag ttacggtttg ccgttgcgcc cgacgagtca
     8881 tgcgttggtt gggcctcgag cgcccggcaa gttgctgatc ccgcgcgacg acgatctggg
     8941 aaccaactgg gtgcgccccg atttcgacga ctccgactgg accccggtcg tcaccggggt
     9001 gggcttcaat gtgccgggcg atttggtcgg gagcgacatc gagggtgcga tgcggggaat
     9061 caatccgacg gcctatttgc gcatcccgtt tgtcgtcacg aacctcgcgg ccctggaccg
     9121 gctgacattg aacatccgtt acaacgacgg gtttgcggcc tatctcaacg gcttcccaat
     9181 cgctcaacgc aacgcgccgg tgacggcgcc tggaggcgtg cttgccaaca gtgccgcgga
     9241 ctggtccttc accggccagc aggggttcaa caactggtat tacgggttct acgatctcac
     9301 ggcggatgcc gatggccgat acgatgccgg tcgtgacttc gtgagttcgc atccgcagtg
     9361 gtcgtggaat ggcggcgcgt gggttctggg ccccgccaat ccgccctggg atgccatcac
     9421 gcaaggcggt tggttctcca acggagagaa cagcggtggg gtgcattggg tgattcgccg
     9481 ctgggtgtgc gaggcgcctg ggacgtttgt ctgtcaggtg gctttcgcga aggagaatcc
     9541 gacctgcggc aatggcgcca ccctccgcgt attcgtcaat ggcaatgagc agtatcaggc
     9601 gactgtcgcc tttgacgaca ccacaggcct gcacaccaat ctcacggtga ccgaccttgc
     9661 cgcgggtgac acgctcgatt tcgcattgga cccgcttggc acagacggcc agcggaccga
     9721 tcactgcgac gaggtgacgt tctcggtcgt catcgatcag attccatccg acggggctgt
     9781 ctggaacaca atggccaatg cggctcgcac gccggacgag accctcgtgc ccgagtcgtt
     9841 cgacctcacg cgctatcggg atgtgctaat cagtggcacg aacgtgctgg ccattcaggg
     9901 cctgaacatc agcgctgacg acgccgactt tctgctggcc cctgaactgg cgggcgggtg
     9961 tctggggctg agcctcgatg agcatgtcta ctttcccaaa ccgactcccg gagaggcgaa
    10021 tggctccggg acggcagcgc tcgggccgat catcagccag gtggaacatc agcccgtcgt
    10081 gccagaggat tcggaggacc tgatcgtccg ggcgcgcgtc acctcgagtt tcggtccggt
    10141 cggcgcagtc acccttcgct atcgcgtcat gtactcgggc gaagcgtcca ttccaatgcg
    10201 cgacgacggc gtccacgagg acggagatgc aggcgacggc atctacgcgg cgcgcatccc
    10261 ggcggcggcg gccggccccg gacagatgat ccgctacgcg attcttgccg cggacgatca
    10321 ggggcggacg agccggctgc cggcctacac cgacccgctg ggcagccccc aataccgcgg
    10381 cacagtcgtg cgcgaccctg cgctcgaagc ctcgcggctt ccggtgttgc attggttcat
    10441 tcaaaacccg tccgcggcga attccgacac acgggccagt ggatccctgt tctttgacgg
    10501 cgagttttac gacaacgtgg gcgcaaccat tcatggccaa tccacgcgcg ggttccctaa
    10561 acgcagttac gatctcgatt tcaacccggg ttacaagttc cggtggtccg acgacgcgcc
    10621 gcgggtcgat gacttgaatc tgctcaccac gtgggcggac aagtcccaca tgcggaatgt
    10681 gctggcacac gagacctatc gcgacgcggg ggcgcagagc cactttgcgt ttgcggtgcg
    10741 ggttcaacag aacggggcgt tcttcagcgt cgccaacctg gtcgagaatg gggatgacaa
    10801 cttcctcaaa cgcctggggc tggacccgcg gggggctctt tacaagatgt acaactcggc
    10861 cgacagcgcc gccggcaacg agaagaagac ccgcaagaac gagggcaccg ccgatttggc
    10921 cgcgctcatc caggggatgt cccaggccgg cgcaaaggcc cgggaggcct tcatgtacga
    10981 caacctcgac gtgccgcaga tgattaactt cctggcggcc aagatcatta cggcggacac
    11041 ggactgttgc cacaagaatt actacctcta ccgggacagc gagggcacac gcgaatggat
    11101 ggcgatgccg tgggacgtgg acctttcttt cgggcgggtt tggactggcg cccatgccta
    11161 ctacgacgag cagatttaca ccaatcaatc gatcttcatt ggcagcggca acatcgtctt
    11221 cagccccatt tacaacacct cggcgacgcg ccagatgttc ctgcgccgct tgcggacact
    11281 gatggacgac cttctccaac cgctcgccac gcccgccaca aacgattcct accggatcaa
    11341 gagccgcgcg ctgcgcgatc agatcgcccc cgacgccgcc ctcgatctcg ccaagtgggg
    11401 gacatggggc acgcgcgaga cgatcaccca ggcggtcgac cgcatctgga atcagttcct
    11461 tcctggacgc cgggtctacc tgttccgcac cctgtccgtc accaacggcg gtgaaatccc
    11521 gctcccgcaa cccgccgatg ccgtcgtcga gatcggcagc ctcgagtacc gccccgccag
    11581 cggcaacccc gccgaggaat ggctctcgat caccaatgcc aacccctatg cggtcgatct
    11641 ctccggctgg caactcgacg gcgccgtccg tttcaccttc aaacccggg
//
LOCUS       DAOQRO010000066        11684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4675_length_11684_cov_4.222031, whole genome shotgun sequence.
ACCESSION   DAOQRO010000066 DAOQRO010000000
VERSION     DAOQRO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4675_length_11684_cov_4.222031"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2845
                     /locus_tag="PKK20_03915"
     CDS             <1..2845
                     /locus_tag="PKK20_03915"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU99063.1"
                     /translation="WASVYFKQVEAGTFSTFEFGEGRNMYGVVVKGLPTSISNPPVIT
                     DLKPVNQTLFYGASGGLSFKASTVAPNRLDPQNLRLVLNGADVSSQLVVGGTSADRTV
                     EFKGLVANTVYVAHITVSDQAGRSASADLSFDTFSDTAATLIEAEDYNYGGGMSMAAA
                     PPAGYAGLTGTPDVDYHDNNTTTAATEYRTADFVGLRVSTDGLRARFTSVEATDYHVT
                     GFVAGDWMNYTRTLDNNTYRVYLRASAGRAQTIRLDRVTGDPGSATQGRSPLGVFAVP
                     NTPYTYVPLSDAAGQPVTLALGGLTTLRLTALESSSPSSQLNFLMLVPVEGPARLPHV
                     SEVVPAMNAADVAQESVVAATIVNGSTPVPPGNVTLTVNGEEVTSAASVTATTDGVQV
                     TYDPPGALAIGAAFSVRVAFTDAGGTAGALDWTFTTRPYAPVITSVVEAGGDDSEFTP
                     AQFTGQTFNHPNLGTITVPTFREDVPAYRDRVHQWNGAAAILPLPSYLVGGDYIMIRN
                     DNRDNGGFQLDVTVAAPALVYVLVDNRLGDSNAANPPEFTTGLMSWFEAEGWVPVRTG
                     HNRLLSPALPDEVGVDESGDGIGAGAGVDSYSSVYVREVPAGTVSLYTADNAGRNMYG
                     VVVRATSTHSFVPVVEITSPVPEATYPTTPASIAIAATASVRDSAVTRVEFFEAVAGK
                     LGETLAAPYNFVWNNVPAGRYRLTASATAANGQVSVSQPVEVIVGTVISVNFQDGTAE
                     TPVGYLMDVGQVFGDQGNGYSYGWDADNSAHARNRNNVISPDERYDTFNHLQKDLPAG
                     RVWEIELPNGNYSVHVVVGESDNYDSTFDIQAEGVTIISGQATATVRWFENTAIVVVA
                     DGRLSLSNGPTAVNNKICYVDIGRLPDTMPPAKFAPPTLSGNTVNLTWTGQGRLQEAS
                     QVNGPWADVAGIPQNSYSVTATAPQKFYRVVFP"
     gene            complement(3111..4445)
                     /locus_tag="PKK20_03920"
     CDS             complement(3111..4445)
                     /locus_tag="PKK20_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99064.1"
                     /translation="MNPPSPSRRSFLKTASVLATAVTVVPRHVLGQGQIPPSQKLNIA
                     GIGVGGMGAGDVASCAQAGENIVALCDPDANALAATAKKYPQAKLYSDFRKMLETQKD
                     IDAVTIATPDHVHAVAASMAMKLGKHVHCQKPLTHSVYEARMLGRIARETKVATQMGN
                     FGQASEEARRVAEMIWAGAIGTLKEVHAWSNRNPDISPRGVPRPKETPPCPAHLDWDL
                     WIGPAPMRPYHPCYHPFAWRGWWDFGTGVLGDIGCHELSAIFKAMKIGDPVSVEACST
                     NWQRPKEISTETAPLASITHYKFAASDTHGPLSIFWYDGGMRPPRPEELDPDAEFAVN
                     DGTMYVGDKGIMLRDRLIPESKRQEFGRPPQKLPRSVGHWKEFLDACRGGQPAGSNLA
                     DHAAHLTEVVLLGNIAIRMNQKLEWDAVNMRFPHCPEADQFINPPYRPGYAL"
     gene            complement(4540..5997)
                     /locus_tag="PKK20_03925"
     CDS             complement(4540..5997)
                     /locus_tag="PKK20_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99065.1"
                     /translation="MGTAPEQPIHIRTLARNGRVKALARAILGWFRSKVDQSTPMERP
                     TAMHSRRLHRGLHVIAGALLAGLGLVPTCFLELAGCWLSLRDPLVESGCIVILLGWVT
                     YRPSIARASAGLALGISLSVLGLGAAECLSRWTRWDFRKTEATLRRLPPYYRKPTVLT
                     GDVFFRRPGPERWKGRVIEHTLRELGIGEDLYADELEVTVHYDAQGFRNPTGLTDWDI
                     AVAGDSFTELGFLPEDLLFTSLLSRRLGLRVKNLGVSHTGPLTQLHYLETYGLGPSTR
                     RVAIMFFEGNDLQDLEVESHRLRWFQQTGKRPSAQPKPQCSLLRAICDLILRPVPPAA
                     PDAAPAPDAYLDVAGKRIAVDFGSVPVAASDVNPKLREALDDFLARYGTWARERGLEA
                     WLFYLPAPLRVWHGRLDFTEQAPEQVRLWQPTDLPDHLEQLVQAHGLRFCDLTPALIE
                     ATHTREVLLFNPRFDTHLNATGSAVVAEAMARAFE"
     gene            6338..7156
                     /locus_tag="PKK20_03930"
     CDS             6338..7156
                     /locus_tag="PKK20_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99066.1"
                     /translation="MKTHMLQWPQPLSRRECRSGFTLIELLVVIAIIAILAGMLLPAL
                     SRAKEKGKSIRCLSNVRQMCLGYFLYADDHNDDVVTLYLFQTAPSGAFYPGGVTWWVD
                     ILRPYLQGTNVTVCPSIRGGVAGTAGGPGGLGVSLSHPELSAWSTEWMPKLSAMKNPV
                     KKTPFVDSGLIANPRERDPDNWVEVSKQQSLYWRVPTNRGYYDDDPQRPVGRHLKRCM
                     AGFADGHGEALRVSALGLQFFPGRAAGGQSATGMSWLGGNNLYDDRWMWSFASD"
     gene            7288..7932
                     /locus_tag="PKK20_03935"
     CDS             7288..7932
                     /locus_tag="PKK20_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99067.1"
                     /translation="MNKRTRDILIAGLLVCAAIVWLRPIFVGSSPKIELGTYQALGAV
                     TAEETAKLMGAKGRVLVIARDTGADKNPSVEAELAAFQQTLKKHGGLSQVTERFVATP
                     MLMMATGGSVPPDRLSEALAAHTDIGALVLFCAFPPLADADLDALRKRRVKTVVVSSF
                     RPEYARLMEQGILSLAIVPRPEAPPEGAPQPRTLRELFDQDYTILAPQDGVSGG"
     gene            complement(8008..8196)
                     /locus_tag="PKK20_03940"
     CDS             complement(8008..8196)
                     /locus_tag="PKK20_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99068.1"
                     /translation="MISFSRGSIWEVVLKRALWLGTNWVSRSSAATNWQWQVRRLNDA
                     LLLVGENGTLRTSSDGVH"
     gene            complement(8193..8921)
                     /locus_tag="PKK20_03945"
     CDS             complement(8193..8921)
                     /locus_tag="PKK20_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99069.1"
                     /translation="MTQTSAQSTAALEALNLTKVYGTGNTEVIAMNDVTLRVARGEIV
                     ALLGPSGAGKSTLLTALGLINPPNSGRIVIGGTPVMDGPRALVDLPAFRRRHLGFVFQ
                     KANLIPFLNAVENVQVALEINDVPPRTARQRAMELLDYLGVADRADNLPDALSGGQQQ
                     RVAVARALANHPSLILADEPTAALDSHRGRQVMELFRKVAHERAAAVLVVTHDHRSLD
                     VFDRTCEMEDGRLRGTVDTSTAMV"
     gene            complement(8918..10129)
                     /locus_tag="PKK20_03950"
     CDS             complement(8918..10129)
                     /locus_tag="PKK20_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU99070.1"
                     /translation="MNLATKDIRHNFGRFALTTVGVGLLLMIVMGMGGIYRGLIREAT
                     LLVDRIGGDFWVVQGSTRGPFAEISRVPPILEDRVRGVPGVASARRFVSHTIQREFRG
                     RPLRIVVQGLAWPDDQGAWLPLIAGRPLGQAHLEMVADRSLGLPLGEKVKLGKDIYEV
                     VGLTSGMVGSGGDGLGFFTVSDAQSIQFDLPGDAIRLDRQSRRARAEAMDMGRVQPLV
                     LERSAGMSDGIPALGPPMVSAVLVALEPGADPHAVAATIASWPDVTVYSEQQQKDLLL
                     SGMVDKARRQLGLFRALLIIISTIIMALIIYTLTLDKVHDIAMLKLIGARNSVIVSLI
                     LQQALLIGALGYGLAYWFGTFAFPRFPRMVTIEAPDLLQLAAIVLGISVLASLLGIWK
                     AVRIEPNKVLS"
     gene            complement(10167..11357)
                     /locus_tag="PKK20_03955"
     CDS             complement(10167..11357)
                     /locus_tag="PKK20_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU99071.1"
                     /translation="MGRGRIGLFLRAIAKWLVPLAVIVFIVVRVKFARVPVLAHEVKA
                     GPIVAEVMGTGTLEARVKTVISPRIQGILSEVLVDQNDPVEAGQLLALLEDQELKLQV
                     DVAESALAAARASAQRVRADEARAQAVVHQAQLEHQRIAGLLTNRISSQADFDKAIEL
                     LNVASADLRRSQAAIVEADSQVQTAEKQLAHQRELLTYTRIVSPYNGLIVRRDRDPGG
                     VIVPGGSLMQVISTNEIWVSAWVDETAMAGLAPGQPARVVFRSEPARSYPGEVARLGL
                     EADRETREFVVDVRVNRLPQVWAVGQRAEVFIETGRKSGVLTVPPQFVQWRNNRPGVF
                     VNERDVAVWRDVTTGLRGQADIEIAQGLSEGEQVVAPRDFQTQLRPGQRIRAGGSIQL
                     KAAP"
BASE COUNT         2153 a         3547 c         3814 g         2170 t
ORIGIN      
        1 gtgggcctcg gtctatttca agcaggtcga ggccggcacg ttttcgacct tcgagttcgg
       61 cgaggggcgg aacatgtacg gtgtggtggt caaaggtctg ccgacctcga tcagcaatcc
      121 gccggtcatc accgatctca agccagtgaa ccagacgttg ttctatggcg catcgggcgg
      181 ccttagtttc aaggcctcga cggttgcgcc caatcggctc gatccgcaga atctgcggct
      241 tgtcttgaac ggggctgatg tctcctcaca gttggtcgtg ggcggcacat cagcggatcg
      301 cacggtggaa ttcaaaggtc tcgtggcaaa cacggtctac gtggcgcaca tcacggtgtc
      361 agaccaagcg ggccgctccg cgagcgccga tctgagcttt gacacgttca gcgataccgc
      421 cgcaactctg atcgaagccg aggattacaa ctacggcggc ggcatgagca tggcggcagc
      481 gccgccggcc ggctacgcgg gtctgaccgg cacgccggac gtcgactacc acgacaacaa
      541 cacgacgact gcggcgaccg aataccgcac ggccgatttc gttgggttga gggtttccac
      601 ggacggtttg cgcgcgcggt ttacgtccgt ggaggccacc gactatcacg tgaccggttt
      661 tgtcgcgggc gattggatga actacacgcg aacattggac aacaacactt accgggtgta
      721 cctgcgcgca tccgccggac gcgcccagac cattcgcctg gaccgtgtca cgggcgatcc
      781 gggttccgcc acccaaggcc gttctcccct gggcgtcttt gcggtgccca acaccccgta
      841 cacctatgtt cccctttccg atgctgccgg tcagccggtc acgttggcac ttggcggtct
      901 gaccacgctc cggctgaccg ctctggaatc atcaagtccc agctcacagt tgaatttcct
      961 catgctcgtc ccggtcgaag gacccgcgcg cctgccgcat gtcagtgaag tcgtcccggc
     1021 catgaacgcg gcggatgtcg cccaggagag cgtcgtcgcc gccacgattg tgaacggttc
     1081 gaccccggtg ccgccaggca atgtgacgct cacggtcaac ggggaagaag tcaccagcgc
     1141 ggcctccgtg acggcgacga ccgatggcgt gcaggtgacc tatgacccgc ccggggccct
     1201 cgccatcggc gccgcgttct ccgttcgtgt cgcgttcacc gatgcgggag gaacggcggg
     1261 cgccctggac tggacgttca cgacccggcc ttatgcgcct gtgatcacga gcgtggtcga
     1321 ggcgggagga gatgattcgg agttcacccc ggcgcaattc accggccaga ccttcaatca
     1381 tcccaacctg ggcacgatca cagtcccgac gttccgggaa gacgtccccg cttaccgcga
     1441 ccgggtgcac cagtggaacg gtgcggcggc gatccttccg ctgccatcct acctcgtggg
     1501 cggggactac atcatgatcc gcaacgacaa tcgggacaat ggcggcttcc agcttgatgt
     1561 cacagtcgcg gcgccggcac ttgtctacgt gctggtggac aatcgccttg gcgactcgaa
     1621 cgcggcgaat ccacccgagt tcaccaccgg cctgatgtcg tggttcgaag ccgagggctg
     1681 ggtcccggtt cggaccggac acaaccgcct tctctcgccg gcgcttccag acgaagtggg
     1741 cgtcgatgag agcggcgacg ggattggggc gggcgcgggg gtggacagct actcgtcggt
     1801 ctatgtgcgc gaggtgccgg ccggcaccgt gtcgctctat accgcggata acgcggggcg
     1861 gaacatgtac ggcgtggtgg tgcgtgcgac gtcgacgcac agtttcgtgc cggtggtcga
     1921 gatcacgagc ccagtgcccg aggcaacgta tcccacgacg ccggcaagca ttgcgatcgc
     1981 agcgactgct tcggtgagag acagtgcggt gaccagagtc gagtttttcg aagcagtagc
     2041 gggcaaactg ggcgaaacgc ttgccgcgcc ttacaacttc gtttggaaca acgtcccggc
     2101 aggccggtac cggttgacgg ccagcgccac cgcggccaac ggtcaagtca gcgtatcgca
     2161 gccggtcgag gtgattgtcg gcacagtcat cagtgtgaac ttccaggatg gcaccgctga
     2221 gaccccggtt ggctatctga tggacgtcgg ccaggtgttt ggggatcagg ggaacggtta
     2281 cagctacggg tgggatgccg acaactcggc acatgcgcgc aatcgcaaca acgtcatctc
     2341 gcctgacgaa cggtacgaca cattcaacca cttgcagaaa gacctgccgg ccgggcgcgt
     2401 ctgggagatc gagttgccca atggcaacta cagcgtccac gttgtcgtag gcgaatcgga
     2461 caactacgac agcacgtttg acattcaagc cgagggcgtg acgattatct ctgggcaagc
     2521 gaccgccact gtgcgctggt tcgagaacac ggccatcgtg gtggtggccg atggccgact
     2581 ctcgctaagc aacggtccga ctgccgtcaa caacaagatc tgctacgtcg atatcggacg
     2641 acttcccgac accatgcctc ccgcgaagtt cgcgccgccg accctgagcg ggaacacggt
     2701 caacctgaca tggacgggcc agggccggct gcaggaggca tcccaggtca atggcccgtg
     2761 ggccgacgtg gcgggcattc cacagaacag ctacagcgtc acagcgaccg cgccgcagaa
     2821 attctaccgc gtggtattcc cgtagaggca taggttgacg cggatgcgcg gccgcggaag
     2881 aggccctgtt ggggaaacgc accggcctcg gatcgaggac tcgcggagct cgtctctccg
     2941 agcttcggtt gcggctgtgc cgcttgaggt tcttcggttc ttcgtggtgt attccagtca
     3001 gtgtcaagca tccagggcga tgatcggggt tttcgtaaaa tggggttgcc ggcctgcctt
     3061 tggcgccaaa acttcactca tttgccacag atttcatctg gggagtgact ctaaagcgca
     3121 tagcccggac ggtacggtgg attgatgaac tgatccgcct ccgggcaatg cgggaagcgc
     3181 atgttcacgg cgtcccattc gagtttctgg ttcatgcgga tggcgatgtt tcccaggaga
     3241 acgacctcgg tgagatgggc agcgtgatcg gccaagttgg agcccgccgg ttggccgccc
     3301 cgacacgcat cgaggaactc cttccaatgc ccgacggagc gcgggagctt ctggggcgga
     3361 cgtccgaact cctggcgctt gctttcgggg atcaggcggt cgcgcagcat gattcccttg
     3421 tcgcccacgt acatggtgcc gtcgttcacc gcgaactcgg cgtcgggatc cagttcttcg
     3481 ggccggggcg ggcgcatgcc tccgtcatac cagaagatgc tcaggggtcc gtgcgtgtcg
     3541 ctggctgcga atttgtaatg ggtgatggaa gcgagcggcg cggtttcggt gctgatctcc
     3601 ttgggccgct gccagttggt cgagcaggcc tcgacgctga ccgggtcgcc aattttcatg
     3661 gccttgaaga tggcggagag ttcgtggcag ccgatgtcgc cgagcacgcc tgtgccgaaa
     3721 tcccaccagc cacgccatgc aaacgggtga tagcagggat gatagggccg catgggagcg
     3781 gggccgatcc agagatccca atcgaggtgc gcagggcagg gcggggtttc cttggggcgc
     3841 ggcacgccgc gcggcgagat gtccggattc cgattggacc aggcgtggac ctccttgagc
     3901 gtgccgatgg cgccggccca gatcatctcg gcgacgcggc gggcctcttc gctggcctgg
     3961 ccgaaattgc ccatttgggt tgcgaccttg gtttcgcggg cgatccgacc gagcatacgg
     4021 gcttcataga cgctgtgggt cagcggcttc tggcaatgga catgtttgcc cagcttcatc
     4081 gccatggagg cggcgacggc atgcacgtgg tcgggcgtgg cgatcgtcac ggcgtcaatg
     4141 tctttctgcg tctcgagcat cttgcggaag tcgctgtaga gcttagcctg cgggtatttc
     4201 ttggcggttg cggccagcgc gttcgcgtcc ggatcgcaca gcgccacgat gttctccccc
     4261 gcctgggcgc acgaggccac gtcgcccgct cccattcctc caacaccgat cccggcgatg
     4321 ttgagtttct gtgaaggggg aatttgacct tgccccagca catgacgggg cacgacggtg
     4381 acagccgtgg cgaggacgga ggcggttttc agaaagctgc ggcgggacgg cgaaggcgga
     4441 ttcatggctt tatcttccac tcgaaggtta tggtctgttt gcgccgagtg tatgtcgttg
     4501 gattcacagg tcaaacatag acgtgaatga aagttgaacc tactcgaacg cgcgcgccat
     4561 cgcctccgcc accacggcgg accccgtcgc attgagatgc gtgtcgaaac gcgggttgaa
     4621 aaggaggacc tcacgcgtgt gcgtcgcctc gatgagcgcc ggcgtcagat cgcagaaccg
     4681 cagcccgtgc gcctggacga gttgttccag atggtcgggc agatcggtgg gctgccagag
     4741 ccggacttgc tccggagcct gctcggtgaa atcgagccgg ccatgccaga ctcggagcgg
     4801 agcgggcaga tagaacaacc aggcttcgag cccgcgctcg cgagcccagg tgccgtagcg
     4861 ggcgagaaag tcatccagcg cttcgcgcag cttcggattc acatccgacg cggcgaccgg
     4921 cacgctgccg aagtcgacag cgatgcgttt cccggccaca tcgaggtatg cgtcgggggc
     4981 gggggcggcg tcgggggcgg cgggcgggac ggggcgcagg atcaggtcgc agatggcgcg
     5041 gagcaatgag cattggggct tgggttgtgc ggaaggacgc tttccggttt gctggaacca
     5101 tcgcagccga tgcgactcga cttcgagatc ctggaggtcg tttccctcga aaaacatgat
     5161 cgccacccga cgggtcgagg ggcccaagcc atacgtctcg aggtagtgca gttgggtcaa
     5221 cggaccggta tggctcacgc cgagattttt cacccgcaag cccaaccgcc ggctcagcaa
     5281 actcgtgaac agcagatcct ccggcaggaa acccagctcc gtgaaggagt cgccggcgac
     5341 cgctatgtcc caatccgtca atcccgtggg gttacggaag ccctgcgcat cgtagtgcac
     5401 cgtgacctcg agttcatcgg cgtaaagatc ctccccgatg cccaactccc gcagcgtgtg
     5461 ctcgatcacc cgtcctttcc accgctcggg tccgggacgg cggaagaaga catcgcccgt
     5521 caggaccgtg ggtttccgat aataaggagg cagccggcgc aaggtcgctt cggttttgcg
     5581 aaaatcccat cgggtccagc ggctcaggca ttcggcagcc cccaacccaa gaacggagag
     5641 actgattccc agggccagcc ccgccgaggc gcgggcaatg gacggacgat aagtcaccca
     5701 acccaacagg atgacaatgc acccgctctc gaccagcggg tccctcaacg acagccagca
     5761 gcccgccagt tcgagaaagc aggtcggcac cagacccaat cccgccaaca gagcgcctgc
     5821 gatgacgtgc agcccgcgat gcaggcgtcg actgtgcatt gcggtcggcc tctccattgg
     5881 cgtcgactgg tccactttgc tcctgaacca gccgaggatc gctcgagcca gcgccttgac
     5941 tcgcccgttg cgcgcaaggg tgcggatgtg gattggttgt tcgggggcgg tgcccatccc
     6001 agtggtctgc gaagcgtcag cgcgcggcta caacagttca cgaatgcgga aatacttcgt
     6061 ggcttcactc gcaccgagaa gtccaagggc caccaggagt tccgatgcag ttctggtgat
     6121 gttcatgtgg tgttcatgtg attcaatccg ttgcagttga tctcattccg gatttcactg
     6181 aagcaccgtg gactctcctt gaaaacatgc gcggttcaag gactatttgg ctcgttttcc
     6241 atcgcggttt ttgttggctc gaggcgcgtg aggctggtat cctgacgctt atcggaagct
     6301 tccatgccat gtcgggcgcg cgttggtcga acgaattatg aagacgcata tgttgcagtg
     6361 gccacaacca ctttcccggc gtgagtgccg gtctggtttc actctgatcg aactgctggt
     6421 cgttattgcg atcattgcga ttctggcggg catgctgttg cctgcgttgt cccgggccaa
     6481 ggagaagggc aaatcgatcc gctgtttgag caacgtgcgg cagatgtgcc tgggctattt
     6541 tctttacgcg gatgatcaca acgacgacgt ggtgacgctg tatttgtttc agacggcgcc
     6601 gagcggggcg ttttatccgg ggggtgtgac ctggtgggtg gacatcttgc gcccttactt
     6661 gcaggggaca aacgtgacgg tgtgccccag catcagggga ggtgttgcgg gcactgccgg
     6721 cggtccggga ggtctggggg tctcgctgag tcatcccgag ctttcggcgt ggtcgacgga
     6781 gtggatgcca aagctctctg cgatgaagaa tccggtcaag aaaacgccgt ttgtcgactc
     6841 gggcctgatt gccaacccgc gtgagcgcga tccggacaat tgggtcgagg tctcgaaaca
     6901 gcaatccctg tattggcgcg tgccgaccaa tcgagggtat tacgacgacg atccgcagcg
     6961 tccggtcgga cgccatctca agcgctgcat ggccgggttc gccgacggcc acggggaggc
     7021 gcttcgagtc agcgcgctcg gccttcagtt tttcccaggg cgggcggccg gcggccagtc
     7081 ggctacaggc atgtcctggc tgggcggcaa caatctgtat gatgaccgtt ggatgtggtc
     7141 ctttgcctcg gattagcccg caggctttca gcggggcttg agtcgcgctg ggaaatgtcc
     7201 tgctagccgg cattcctgac tacttttaga catcgggaag ccggaacacc gaaaagaact
     7261 accgatgcgg gttaggcgga ccctgacatg aacaagcgaa cacgagacat tctgattgcc
     7321 ggccttctcg tctgtgccgc tatcgtttgg ctgcgcccga ttttcgttgg gagttcacct
     7381 aaaatcgagt tgggcaccta ccaggcgctg ggcgcggtga ccgctgagga aacggcgaag
     7441 ctcatgggag cgaaggggcg ggtgcttgtc attgcccgcg ataccggggc cgacaagaat
     7501 ccgtccgtgg aagccgaatt ggcggcattc caacagacgc tgaagaagca tggagggctg
     7561 agccaggtca ccgagcggtt tgtagcgacg ccgatgctga tgatggccac cggcggcagc
     7621 gttcctccag atcggctgtc ggaggcgttg gcggcccaca ccgacatcgg cgctcttgtt
     7681 ctcttctgcg ctttcccgcc cctggccgat gcggacctcg atgcgctcag gaaacggcgt
     7741 gtcaaaaccg tcgtggtttc ttcgtttcgt cccgagtatg cgcggctgat ggagcaggga
     7801 atcctcagtc tcgcgattgt tccgcggccc gaggccccgc cagagggggc tccgcaaccg
     7861 cggaccctgc gcgagctgtt tgatcaggac tacacgatcc ttgcaccgca ggatggggtg
     7921 agcggcggct gacggggcga caaaccatgc gtctccgaca taagcggcat cggtgagctt
     7981 ctccgcggtg tcgttggctt gcgccgtcta gtggacgccg tcggagctgg tgcgcagcgt
     8041 gccgttttca ccgacgagca gcagggcatc gttgagccgg cggacctgcc actgccagtt
     8101 cgtcgccgcg ctcgagcgcg agacccagtt tgtcccaagc caaagagccc gtttgaggac
     8161 aacctcccag atcgagcctc gggaaaaaga gatcacacca tcgcagtcga ggtgtcgacc
     8221 gtgccgcgca ggcgcccgtc ctccatctcg cacgtgcggt cgaagacatc gagggaccgg
     8281 tggtcgtggg tgacgacgag gacggccgcc gcccgttcgt gagcgacttt gcgaaacaac
     8341 tccatgacct gtcgaccgcg atggctgtcg agcgcggccg tgggctcgtc ggccaggatg
     8401 aggctggggt ggttggccag ggcacgagcg acggcgaccc gttgttgctg gccgccggag
     8461 agggcgtcgg gcaggttgtc ggcgcggtcg gcgacaccaa ggtaatcgag cagttccatg
     8521 gcgcgctgtc tggccgtgcg aggcgggacg tcgttgattt cgagggcgac ctggacgttc
     8581 tcgacggcgt tcaggaacgg gatgaggttg gccttctgaa agacaaagcc cagatgccga
     8641 cgtctgaaag cgggcaggtc cacaagggcg cgaggcccat ccatgaccgg tgtgcctccg
     8701 atgacgatcc gacccgagtt gggcggattg atcaggccga gcgcggtcag tagagtcgac
     8761 ttgccggcac cacttgggcc gagcaaggcg acgatttcgc cgcgggcgac gcgcagggtg
     8821 acatcgttca tggcgatgac ctcggtgttg ccggtgccgt agaccttcgt gaggttcagg
     8881 gcttccaggg cagcggtcga ttgcgcggat gtctgcgtca tgacaagacc ttgttgggct
     8941 cgatgcgcac ggctttccag atgccgagca gactggcgag caccgagatc cccagaacaa
     9001 ttgcggcgag ttgcaggaga tcgggcgcct cgatcgtcac cattcgggga aagcgcggga
     9061 aggcgaacgt gccgaaccag taggccaggc cgtagccgag cgcgccgatt aacaacgcct
     9121 gctgaaggat gagactgaca atgacgctgt tccgcgcgcc gatgagcttg agcatggcga
     9181 tgtcatggac cttgtcgagg gtaagggtat agatgatcag ggccatgatg atggtcgaga
     9241 tgatgatgag cagggcgcga aacagaccca actggcgtct ggccttgtcc accataccgc
     9301 tcaggagcag gtccttttgc tgctgctctg aatagacagt cacatcgggc caactggcaa
     9361 tcgtggcggc cacggcgtgg ggatcggccc cgggttcgag cgccaccagc acggcgctga
     9421 ccatgggcgg ccccagcgcc ggtatgccgt cggacatgcc cgcactgcgt tcgagcacga
     9481 ggggttgcac ccggcccata tccatcgcct ccgcccgggc tcgacggctt tggcgatcca
     9541 ggcgaatcgc gtcgccaggc agatcgaact ggatgctctg ggcgtcgctg acagtgaaga
     9601 aacccaggcc gtcgccgccc gatccgacca tgccgcttgt gagccccacg acttcgtaga
     9661 tatctttgcc cagtttcacc ttttcgccca gcggcaggcc gagcgaacgg tcggcgacca
     9721 tttccaagtg cgcttggccc agcgggcggc ccgctatcaa cggcagccac gccccctgat
     9781 cgtccggcca ggccagtccc tgcacaacaa tgcgaagagg gcgcccgcgg aattcgcgct
     9841 ggatcgtatg cgaaacaaag cggcgtgcgc tggccacgcc cggcaccccg cgcacacggt
     9901 cttcgagaat ggggggcacc cgtgaaatct cggcaaaggg gccgcgcgtg ctgccttgga
     9961 ccacccagaa atcgcccccg atcctgtcga ccaacagtgt ggcctcgcgg atcaaccccc
    10021 tgtagatgcc gcccattccc atcacgatca tcagcagcag accaacaccc actgtcgtga
    10081 gggcgaaccg cccgaagttg tggcggatgt ctttggtggc gaggttcacc gggatgacag
    10141 gatttcaagc gccgggagga aatgcctcac ggcgcggctt tcaattggat cgatccgcct
    10201 gcgcgaatgc gctggccggg gcgcagttgc gtctggaaat cgcgcggggc gaccacctgt
    10261 tcgccctccg acagaccttg cgcgatctcg atatccgcct ggccgcgcag cccggtagtg
    10321 acatcccgcc aaaccgcgac gtcgcgctcg ttcacgaaca ccccgggccg gttgttgcgc
    10381 cactggacaa actgaggcgg caccgtcaac accccgctct tgcgcccggt ttcgatgaac
    10441 acctcggcac gctgtcccac cgcccagacc tgcggaagcc ggttcacacg cacatcgaca
    10501 acaaattccc gtgtctcgcg gtcggcctcg aggcccagac gcgccacttc gccggggtaa
    10561 gatcgggctg gttcggatcg gaacaccacc cgagctggct gtccgggggc gaggccggcc
    10621 atcgcggtct catcgaccca ggccgaaacc cagatttcgt tggtcgatat cacttgcatc
    10681 agagaaccgc cggggacgat gacgccgccg ggatcgcggt cgcgccggac aatcaggccg
    10741 ttataggggc tgacaatgcg agtgtaggtg aggagttccc tctggtgcgc gagctgtttc
    10801 tcagcagtct gcacctggct gtccgcctcg actatcgcgg cctgcgagcg gcgaagatcg
    10861 gcgctggcca cattcagcaa ctcgatcgcc ttgtcgaagt ccgcctggga actgatccgg
    10921 ttcgtaagca atccggcgat tcgctgatgt tcgagttgcg cctggtggac aaccgcctgc
    10981 gcgcgcgctt cgtcggcccg cacgcgctgg gcggatgccc tggcggcggc cagggcagat
    11041 tcggcgacgt cgacctgcag cttcaattcc tggtcctcga gcagggccag caattggccc
    11101 gcctcgaccg ggtcattctg atcaacgagg acttcggaga gaatcccctg gatgcgcgga
    11161 ctgataactg tcttcaccct ggcttcgagt gtgcctgtgc ccatcacttc cgcgacgatc
    11221 ggaccggcct tgacctcgtg ggcaagaacc ggaactcgag caaacttgac gcgaacgaca
    11281 ataaaaacaa tgaccgctag cggcaccagc cacttggcga tcgctcgaag aaacagtccg
    11341 attcggccac gacccatgtc aggcgctatc gagccgcgaa tgcacgcaca gttacaagct
    11401 atttgtgggg acagcaattc ggcggagcga catctgcgat gcagcaccgg gtcagggaat
    11461 cccgtccagt gtgcctgctc gtgcactgga gcacgcagga gtggagacgt cctagtcccc
    11521 ggcggatggg atcgcagaat gcgttcgcgg gcgacgaggg cgtcgcccga cccgatcaca
    11581 cgctgtcgat gctcatagtg tggacaatct ggggtggtca ggagagaaag aatcgaggaa
    11641 cctgaccacc aatgagcagg cggcgtgccc tcacgcggca gaac
//
LOCUS       DAOQRO010000067        11681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4678_length_11681_cov_4.212197, whole genome shotgun sequence.
ACCESSION   DAOQRO010000067 DAOQRO010000000
VERSION     DAOQRO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_11681_cov_4.212197"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PKK20_03960"
     CDS             complement(<1..1150)
                     /locus_tag="PKK20_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99072.1"
                     /translation="MKPHNDAPSIRPQGAVRSLPTTLLLTLLCAWTLPTGQAATSIFA
                     DDFEGHETAATSLADMSDADPTHPNMRVIDDDPFGADPGSGVQIVNWQANSGSKALLL
                     RSGSEAQVHFTQARSGSSYQFDFWLYTVKGAGDRNFYLILRGEGSDYNGDDYLAYRSD
                     RAATKNIFYYDGVGPGAAAWVNTGVNHTDEVWQHHRIIIDPNALTFDLYLDNMDTPVV
                     SGAELSRCEVAVPTMLRIIHEGNSDDDGYFLIDDISLTVEGAIDLTTPFTEGFESYDA
                     RTSVDDDADPKGPWITTEINGTGSGKDRAPAKVQVVDSTVVTPHSGEKCLKIEGGQRA
                     GATVAWGEPAQSDVQITWWAYVPASVDATTATYLRMSLYGAEDGNCIAG"
     gene            complement(1418..1651)
                     /locus_tag="PKK20_03965"
     CDS             complement(1418..1651)
                     /locus_tag="PKK20_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99073.1"
                     /translation="MESPPPTPRRTRQEQIVEQHHTLRPDQTAIEFSNAEEMIRFDAA
                     QTLPPPALGARVRNSVNREPRVQRSWWMRLLGR"
     gene            complement(1659..2237)
                     /locus_tag="PKK20_03970"
     CDS             complement(1659..2237)
                     /locus_tag="PKK20_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU99074.1"
                     /translation="MQSALAMSDLVEAKRSPVPAQSAWESTGFGAIYASHSQAIYYLA
                     LRLLSDPTQAEDAAHDVFLKAYRHYDRFRGDSAVRTWLYRITINHCRNLQQSWHARTM
                     VSQPDATFWDQTPGAAESPLRILETKELGQRIQTTLDALPEEYRLLLLLVADEQLSYG
                     EVAHLTGQTADAVRGKLHRARKAFATAFSKTA"
     gene            complement(2304..3539)
                     /locus_tag="PKK20_03975"
     CDS             complement(2304..3539)
                     /locus_tag="PKK20_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNU99075.1"
                     /translation="MLWVIAQMAVVMLAGGKAMKGMFGLDETTTIVCLALLAGSYTIY
                     GGLISVAWTDFLQFVVMMLGGLVVTVLGLHQVGGLGELMHAAPEKFKIIYPITDKDYP
                     WFGVWTLFISIGIWYNCSNQFIVQRCLGARSEWDARMGVVFAGFMKILLPLLVVVPGI
                     VAFKLYPGLPDKDQAFPTLVRELVPVGLSGIVMAGLASGMLSHISSVLNSCSTVFTMD
                     LYRPLLGRERSEAHLVLVGRASAFVILAVATGLALWFTRRQLGVFDLIQNIGAWVAAP
                     IAAIFLLGVLWERANAVAATTVLVLAFPYTWLVESVLFKRVPWLQPFDNWLNRTFVVW
                     ATCMALTVIISLLTRPPDPVRIRGMIWSWDYARLPEAERTLNRGVRNLFLWWCLFVGT
                     MAVLYAYVIWFQFWGPASR"
     gene            complement(3577..3918)
                     /locus_tag="PKK20_03980"
     CDS             complement(3577..3918)
                     /locus_tag="PKK20_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99076.1"
                     /translation="MMNHRLDFVLFGLYLLFNIGLGLWVARRKTGGTRGYFLAGEGLP
                     WYTIGGSIIASNISTEHFIGMIGVAYAVGFVVAQWEWGNWFTFTALIWIFLPYYIRGG
                     LYTMPVSRTPL"
     gene            complement(3922..5250)
                     /locus_tag="PKK20_03985"
     CDS             complement(3922..5250)
                     /locus_tag="PKK20_03985"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99077.1"
                     /translation="MRATPPNTRPLQRRAQLVLTAMFLGLIAGVPISQTALELVRGQR
                     IQFTSVFRDAPTSANLRRYEAALKEKSWAQQTFRPVIQRLMFQAFGDAGAKAIKGRDG
                     WLFYRPDVRFLIEGSRPDTEDAASRWVQPAAPVAQWESVARAIRRFHDQLEQRGIRLL
                     IVPVPGKPSVHAEQLTARAGAGAAGYASSTRDLIAELERHGVATVDLLSVFERARSNA
                     PAEKLYLAHDTHWTPHGARLAAEAVAARLRNLGWAPPATTTFTLRKVAIDRWGDILEM
                     MQIPGLHDMFAPQRIDCEQVLDPVLGPLVPGPSERPGTYRHPIHQSPLLLLGDSFSRI
                     YQLPEPRSLGMAAAGTKSPEPESGDRRSSKRLLPGSAGFVSHLAHALRAPVDTIVSDG
                     GASTDVRRKLSINPEILEGKRVVIWQFVERDIALGRAGWADVPLPVELGK"
     gene            complement(5250..6656)
                     /locus_tag="PKK20_03990"
     CDS             complement(5250..6656)
                     /locus_tag="PKK20_03990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNU99078.1"
                     /translation="MVFTTPIFLFYYLPLFLLIYFNLPYRWRNCWITLASYVFYGWWE
                     PWFVMLMAFTTVADFIWGHVITRPTATRAQQRAAVTACVVTNLCLLGFFKYFMFAAET
                     FNVLRETFGAEPVRILRVTLPIGISFYTFHSLTYIIDLYRGHATPARSFTDFSAFVAL
                     FPDLVAGPIIRYKTLAAQLTLREHSVERFASGVAIFCLGFTKKIVLANPCGEVADAVF
                     TAAHPPVLDAWIGVLAYAFQIYFDFCGYSDMAVGLGRMLGFDFLKNFDAPYRSESITE
                     VWRRWHISLSSVLRDYLYYPLGGNRSGPARTYVNLAVVMLLGGLWHGAKWNFIAWGAF
                     HGLWLAFERWRGKRSAYHSWPRPARIGLTFLLMLFSWVLFRADNLGAARDSFAAMFGL
                     GPAPGAAVLLAAELYRPFPLLIMAICGLLVVQPVQAHDWSLEPLTFARASLLLGLFAL
                     AVLFMFSQDFNPFLYFQF"
     gene            complement(6666..7169)
                     /locus_tag="PKK20_03995"
     CDS             complement(6666..7169)
                     /locus_tag="PKK20_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99079.1"
                     /translation="MEARGPGPLRGIATIALVALSLWWLAKRGQTGADEQIGKLGRLE
                     VTARLLERPDAFPDLGAYRYTYVLPYQVIEVHRPDPAGGHRLAPGDPIFVGHYKPWLP
                     RSAIQDGDWGDAPLGGRLDRFVAGESHRMALDYALEDLAPSGALDYCFPPSTNRFFAV
                     WTNPAGF"
     gene            complement(7171..8310)
                     /locus_tag="PKK20_04000"
     CDS             complement(7171..8310)
                     /locus_tag="PKK20_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99080.1"
                     /translation="MEFASSNRSGRDHPPSRPPAGNALPARAPKLRLRTGWLLLAGAL
                     LSLPLGSAQSPDPDSRKLADEVGRLGYLVFAARTAQGDYDLFLSRPDGTARRNLTSTP
                     EWSEFGGRFSPDGRRMLYRRLKKGPDVAPGEGINHDVWGAQGSLVLAEADGSNPQVWG
                     TEGEWPWASWSPDGTRLACLYRRQGRIRIIDILTRQTLKELPRQGIFQQMYWSPDGKR
                     LCGTANFSGQDWNIVSIELETGKATQVSRGLNCTADWFQGDPLRLIYSHRLPGLATSY
                     GWTMLMEGTADGTERNLLYGERGRHVYFGCTSPDSRYIIFSVPQTDGGTDAQMAIIRR
                     ADAPIIVPDDYKELQALCPGARSGPVFRLPLAGFEPHWGDPEIRP"
     gene            8865..10202
                     /gene="gdhA"
                     /locus_tag="PKK20_04005"
     CDS             8865..10202
                     /gene="gdhA"
                     /locus_tag="PKK20_04005"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNU99081.1"
                     /translation="MGLIQDTLDVVRKRDANEPEFLQAVTEVFESIEPAIQRHKKYRD
                     ANILLRIVEPERVVMFRVPWQDDKGQFRVNRGYRIQMNSAIGPYKGGLRFHPTVNLGI
                     LKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGRSDHEVMRFCQSFMIELCRHIGADTD
                     VPAGDIGVGGREIGYLYGQYKRIANEFTGVLTGKSVSWGGSLIRPEATGYGCVYFALE
                     MLKTRGDSLEGRICAVSGSGNAAQYTVEKLIQVGAKPVTMSDSNGFIYDKEGIDAERL
                     EWIKDLKNVRRGRIKEYAEKFGAKYVEGQRPWLVDCHCAFPCATQNEISGEEANRLIS
                     GGCFLVSEASNMPSEPEAIHLFTNKKILYGPGKAANAGGVATSGLEMTQNAMRLPWSR
                     EEVDRRLRDIMATIHKNAWETAIEYDRPGNLVVGANIVGFVKVADAMLEQGLV"
     gene            10218..11351
                     /locus_tag="PKK20_04010"
     CDS             10218..11351
                     /locus_tag="PKK20_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNU99082.1"
                     /translation="MSAIDGLQRPSRPWLRSVPGGALPARLPSFLLLLLGWAILTGGC
                     ASKPAPPLERHEFSEPQMGIPFRIVLYAPNPASARASARAAFDRIAALNLVLSDYEDE
                     SELTRLNRTAGSGRAVAVSDDLWRVLSRAQEMARRTGGAFDVTVGPCVQLWRRARRQR
                     AWPEASRLEAARGAVGFQHLELDPLRRTALLRRPRMRLDVGGIGKGYALQEALETLEA
                     RGIRRALVIGGGDMVAGDPPPGKTGWQIEIAPLDATNAPPAQFVLLRRQALATSGDLF
                     QRFEFGGRRYSHIVNPRTGIGLTDHSLVTVIARDGMTADALSTAVSVLGPDAGMRLVD
                     RERGAAAHVVREVDGILERRVSRRFRAGRNARGECACAGPARW"
     gene            11429..>11681
                     /locus_tag="PKK20_04015"
     CDS             11429..>11681
                     /locus_tag="PKK20_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNU99083.1"
                     /translation="MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILEN
                     VRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITA"
BASE COUNT         2194 a         3461 c         4047 g         1979 t
ORIGIN      
        1 cgccggcgat gcagttgccg tcctcggcgc cgtagagcga catgcgcagg taggtggcgg
       61 tcgtggcgtc gaccgaggcg ggaacatagg cccaccaggt gatctgaacg tccgattgcg
      121 caggctcgcc ccaggcgacc gttgcgcccg cccgctggcc cccttcgatc ttgaggcact
      181 tctcgccgga atgaggcgtc accacggtcg agtcgacgac ctgaaccttg gcgggcgcgc
      241 ggtccttgcc cgacccggtc ccgttgatct cagtcgtgat ccacggaccc ttcgggtcgg
      301 cgtcatcgtc gacggacgtt cgggcgtcgt agctctcgaa gccctcggtg aagggcgtgg
      361 tcagatcgat cgcgccctcg actgtcaggc tgatgtcgtc gatgaggaag taaccgtcgt
      421 cgtcgctgtt gccctcatgg atgatgcgca gcatcgtcgg cacggccact tcgcagcggg
      481 agagttcggc tccgctgacg accggggtgt ccatgttatc cagatagagg tcgaaggtca
      541 gggcattggg atcgatgatg atgcggtgat gctgccagac ctcgtcggta tgattgactc
      601 cggtgttgac ccaggcagcc gcgcccggcc ccacgccgtc gtaatagaaa atgttcttcg
      661 tggcggcgcg gtcggatcgg tacgccaggt agtcgtctcc gttgtagtcc gagccttcgc
      721 cgcgcaagat gaggtagaag ttgcggtcgc cggcgccctt gacggtgtag agccagaaat
      781 cgaactgata ggaactgccg ctgcgggcct gggtgaaatg gacctgggcc tcgctgcccg
      841 aacgcagcag cagggctttg ctgcccgagt tggcctgcca gttgacgatc tgcacaccgc
      901 tccccgggtc agccccgaac gggtcgtcgt cgatgaccct catgttcgga tgcgtgggat
      961 cggcatccga catgtcggcc agactcgtcg ccgccgtctc gtgaccctcg aaatcgtcgg
     1021 cgaagatgga cgttgcggcc tggccggtcg gcagggtcca ggcgcacagg agcgtgagca
     1081 gaagcgtggt cgggagggat cggacggcgc cttgagggcg gatggatgga gcgtcgttgt
     1141 gtggtttcat gttcatattg cgcttggttt cactcttcgt gcggcaaccc cgagtcagca
     1201 gagcccttcg gacgaaggtc agtttacaga cactagggaa ccaatacgga ctcgatatac
     1261 agtcgtcaac ccgtttttcc cctctgcgcg tcgatttcag ccagaggcaa ctgatctggc
     1321 tgcatgggcg tgtcctggaa gggccatgcg actgcaacgg cgctcgagcg ccagccgaag
     1381 gccgccgtcg agccgattcc gggcgatgcg cgtctcttta tcgcccgagg aggcgcatcc
     1441 accaggagcg ctggacgcgg ggttcgcggt tgacggaatt ccggacgcgc gcgccgagtg
     1501 cgggcggggg gagggtctgg gcggcgtcga aacgaatcat ctcctcggcg ttcgagaact
     1561 cgattgcggt ctggtcgggc cgcagggtgt ggtgctgctc gacaatctgt tcctgacggg
     1621 tgcggcgtgg ggttggaggt ggcgattcca tagcgccctc atgcggtttt ggagaatgcg
     1681 gttgcgaagg ccttgcgcgc ccggtgcagt ttgccgcgga cggcgtcggc ggtttggccg
     1741 gtgaggtgtg cgacttcgcc atagctgagc tgttcatcgg cgaccaggag caagaggagc
     1801 cggtactctt cgggcagggc gtcgagtgtg gtctggatgc gctggccgag ttccttggtc
     1861 tcgaggatgc gaagcgggct ttcggcagcg cccggcgtct gatcccagaa ggtggcgtcg
     1921 ggctgactca ccatggtgcg agcgtgccag gactgctgaa gattgcggca gtgattgatc
     1981 gtgatgcggt agagccaggt gcgcacggcg gagtcgcccc ggaaccggtc atagtgacga
     2041 taggccttca agaagacatc gtgagccgcg tcctccgctt gggtcggatc gctcagcaac
     2101 ctgagagcga ggtagtaaat ggcctgcgaa tgggaggcgt agatcgcccc aaaaccagtg
     2161 ctttcccacg cggactgggc ggggaccggc gaccgcttgg cttcgacaag gtccgacatg
     2221 gccaacgcag actgcacgga agattggacc gcaagagcag gggaaagtca cacaggaaat
     2281 tgcgatgttc gcgcccggcc cgatcatcga ctggccgggc cccagaattg gaaccagatc
     2341 acgtaagcgt aaaggacggc cattgtgccg acgaacaggc accaccagag gaagagattg
     2401 cgcacgccgc gattgagcgt ccgttccgcc tcgggcaggc gcgcgtagtc ccaggaccag
     2461 atcatgccgc ggatgcggac gggatcgggg gggcgcgtga ggagggagat aatgacggta
     2521 agcgccatgc aggtcgccca gacgacgaaa gtacggttca gccaattgtc aaacggctgc
     2581 agccagggca cccgcttgaa gaggaccgac tctaccaacc aggtgtaggg aaaggcgagc
     2641 accaggaccg tggtcgccgc caccgcattg gcgcgctccc acaggacgcc caggagaaag
     2701 atcgcggcga tgggcgcggc cacccaggcc ccgatgttct gaattagatc gaacacgccc
     2761 agttgccggc gggtgaacca gagggcgagt ccggtcgcca ccgccaggat gacaaaggcg
     2821 ctggcgcgtc cgaccaggac aaggtgggct tcggagcgtt cgcgtccgag cagagggcgg
     2881 tagagatcca ttgtgaagac cgtgctgcac gaattgagga cggaactgat gtgggagagc
     2941 atgccgctgg ccagaccggc catgacaatg ccgctgaggc cgaccggcac cagttcgcgg
     3001 accagggtcg ggaaggcctg gtccttgtcc gggaggccgg ggtagagctt gaaggcgacg
     3061 atcccgggaa cgaccaccag cagcggcagc aggatcttca tgaacccggc gaagacgact
     3121 cccatgcggg cgtcccattc gctccgggcg cccaggcagc gctggacgat gaattgattc
     3181 gagcaattgt accagatccc gatcgagatg aacaacgtcc agacgccgaa ccacgggtag
     3241 tccttgtcgg tgatggggta gatgatcttg aacttctcgg gcgcggcgtg catcaactcg
     3301 cccaggccgc cgacctggtg caagccgagc acggtcacca ccaggccgcc gagcatcatg
     3361 acgacaaact gaagaaaatc ggtccacgcc acggagatga ggccgccata gatcgtgtag
     3421 ctgccagcca atagcgccag gcagacaatg gtcgtggttt cgtcgaggcc gaacatgcct
     3481 ttcatggcct tgcctccggc gagcatgacg accgccatct gggcgatgac ccaaagcacc
     3541 agcgagcaaa tcgcgaagag atagcggcag gcggcgttat agcggcgttc gagaaacggg
     3601 catcgtgtag agtccgcccc ggatgtagta gggcaggaat atccagatca gcgcggtgaa
     3661 cgtaaaccaa ttgccccatt cccactgggc gacgacaaag ccgaccgcat acgccacacc
     3721 gatcatgccg atgaaatgct cggtgctgat gttagacgcg atgatcgagc cgccgatggt
     3781 gtaccaggga agaccttccc cggccagaaa gtagccgcgc gtgccgcccg tcttgcgcct
     3841 cgccacccaa agccccagtc cgatgttgaa tagcaggtat aggccgaaca gaacgaaatc
     3901 gagtcgatgg ttcatcatga atcactttcc caactcgacc ggcaggggca catcggccca
     3961 cccagcccgg cccagcgcga tgtcgcgctc gacgaattgc cagatgacca cgcgcttgcc
     4021 ttcgagaatc tccgggttga tgctgagctt gcgtcggacg tcggtcgacg ccccgccgtc
     4081 gctgacaatg gtgtccaccg gcgcgcgcag ggcgtgcgcc agatgcgaga cgaatccggc
     4141 cgacccgggg agcaggcgct tggaggaccg ccgatcgccg ctctcgggct cgggggattt
     4201 cgtgccggca gccgccatgc ccagggatcg cggttcgggg agctgataaa tccgggagaa
     4261 actgtcgcca agcagaagca gaggcgattg atggatcggg tgccgatacg tgcctggtcg
     4321 ttcggaaggg ccggggacga gcggaccaag gacgggatcg agcacctgtt cgcagtcgat
     4381 tcgctgcggg gcgaacatgt catgcaggcc cgggatttgc atcatttcga ggatgtcgcc
     4441 ccaacgatcg atggcgacct tgcgcagtgt gaacgtggtc gttgccgggg gcgcccaacc
     4501 cagattccgc aatcgcgccg cgacggcctc ggcggccaag cgcgcgccgt ggggcgtcca
     4561 gtgcgtgtcg tgcgccaggt acagcttttc ggcgggcgcg ttcgatcgcg ccctctcgaa
     4621 cacggagagc aagtcgaccg tagccacccc gtgcctctcg agttcggcga tcaggtcgcg
     4681 ggtcgaggac gcatagccgg cggcgcccgc gccggctcgg gccgtgagtt gttcggcgtg
     4741 gacgctcggt ttgcccggca cgggcacgat cagcaaccgg atgccgcgct gttcgagttg
     4801 atcgtggaat cgtcggatgg cgcgcgcgac gctctcccac tgcgcgacgg gcgccgcggg
     4861 ctggacccag cgcgacgcgg cgtcttcggt gtccgggcgg ctgccttcga tcaggaatcg
     4921 gacatcggga cgatagaaga gccagccgtc gcggcctttg atcgccttgg cgccagcatc
     4981 gccaaatgcc tggaacatga gcctctggat cacaggccgg aacgtctgtt gcgcccagga
     5041 cttctctttc aatgcggcct cgtaacgccg aaggttcgcg gatgtgggag cgtcgcggaa
     5101 cacgctggtg aactggatcc gctggcctcg gaccagttcg agcgccgtct ggctgatggg
     5161 aacgccggcg atcaacccca gaaacatagc cgtcaagacc agttgcgcgc gccgctggag
     5221 cgggcgggtg ttaggagggg tcgcgcgcat tagaactgga agtagaggaa cgggttgaag
     5281 tcctgggaga acatgaacag caccgcgagc gcgaagagtc cgagcagcag cgacgcgcgc
     5341 gcgaaggtca acggctcgag cgaccagtca tgagcctgca ccggctgaac gacaagcagg
     5401 ccgcagatgg ccatgatcag gagcggaaac gggcggtaca gctccgccgc gagcaggacg
     5461 gcagcgccgg gggccgggcc caggccgaac atggcggcga aggaatcccg cgcggcgccc
     5521 aggttgtccg cgcgaaagag cacccacgag aagagcatga gcaggaaggt caggccgatg
     5581 cgcgcagggc gcggccagga gtggtaggcg ctgcgcttgc cgcgccaccg ttcgaatgcg
     5641 agccacaggc cgtggaaggc gccccaggcg atgaaattcc atttggcgcc atgccagagg
     5701 ccccccagaa gcatgacgac ggcgagattc acataggtgc gcgccggccc cgagcggttg
     5761 ccgcccaggg gataatagag gtagtcgcgc aggacactcg acagcgagat gtgccaccgc
     5821 cgccagacct cggtgatgct ctccgagcgg tacggagcgt cgaagttctt gaggaaatcg
     5881 aacccgagca tacgtccgag cccgacagcc atgtccgagt agccgcaaaa atcgaagtag
     5941 atctggaagg catatgcgag cactccgatc caggcatcga ggaccggcgg atgcgcggcg
     6001 gtgaagacgg cgtcggcgac ttcgccgcac gggttggcca ggacgatttt ctttgtgaag
     6061 cccaggcaga agatggcgac gccgcttgca aagcgctcga cgctgtgttc gcggagggtc
     6121 aactgggcgg cgagtgtttt gtagcggatg atgggtccgg cgaccagatc gggaaacaga
     6181 gccacaaagg cggagaaatc cgtgaatgat cgggcgggag tcgcatggcc gcggtagagg
     6241 tcaatgatgt aggtcaggga gtggaacgtg tagaacgaga tgccgatggg cagggtgacg
     6301 cgcaggatgc gaaccggttc ggcgccgaaa gtctcgcgca ggacgttgaa agtctccgcg
     6361 gcgaacatga agtacttgaa gaagccgagc aggcacaagt tggtgaccac gcaggccgtc
     6421 acggccgcgc gttgttgggc gcgcgttgcc gtggggcgcg taattacgtg tccccagatg
     6481 aagtcggcga cggtggtgaa cgccatgagc atgacgaacc acggctccca ccatccgtaa
     6541 aacacgtagc tggccagggt gatccagcag ttgcgccagc gatagggcag attgaagtag
     6601 atgaggagga acagcggcag gtagtagaac aggaaaatgg gggtggtgaa caccatgccc
     6661 gcgcctcaga aacctgccgg gttggtccag accgcgaaga aacggttggt cgacggcgga
     6721 aagcagtaat cgagcgcgcc gctcggggcg aggtcttcga gcgcgtaatc gagcgccatg
     6781 cgatgcgatt cgcccgcgac gaagcgatcg agcctgccgc ccagaggcgc atcgccccag
     6841 tcgccgtcct ggatcgccga gcgcggcagc cacggtttgt agtggccgac aaagatggga
     6901 tcgcccgggg cgagacggtg cccgcctgcc gggtcggggc gatggacctc gatcacctgg
     6961 tagggcaaaa cgtaggtgta gcggtacgcg cccagatcgg gaaacgcgtc cggacgttca
     7021 agcagccgtg ccgtgacttc gaggcgacca agcttcccga tttgttcgtc ggcgccggtc
     7081 tggccccgct tcgccagcca ccacaggctg agggcgacca gcgcgatcgt cgcgatcccg
     7141 cgcagcgggc ctggaccccg ggcctccatg tcagggcctg atctcaggat cgccccagtg
     7201 cggctcgaat ccggcgagcg gaagacggaa gaccgggccc gagcgagcgc ccgggcagag
     7261 ggcctgcagt tccttgtagt cgtcggggac gatgatgggc gcgtccgcgc gccggatgat
     7321 ggccatctgg gcatcggtgc ccccgtcggt ttgcgggacg gaaaagatga tgtagcggct
     7381 gtcgggcgat gtgcagccga aatagacgtg ccggccgcgt tcgccgtaga ggaggttccg
     7441 ctcggtgccg tcggccgtgc cttccatcag catggtccag ccgtaactgg tggcgaggcc
     7501 gggcagacgg tgtgagtaga tgaggcgcag cggatcgccc tggaaccaat cggccgtgca
     7561 attgagtccg cgcgaaacct gggtcgcttt gccggtctcg agttcgatcg agacgatgtt
     7621 ccagtcctgg ccgctgaaat tggcggtgcc gcacagccgc ttgccgtcgg gcgaccagta
     7681 catttgctgg aagatgccct gtcgcgggag ttctttgagc gtctggcgtg tgaggatgtc
     7741 gatgatccga atccggccct gacgccgata aaggcaggcc aaccgggtgc catcggggct
     7801 ccagcttgcc cagggccact cgccttcggt tccccagacc tggggattgg agccgtcggc
     7861 ctcggccagc accagactgc cctgggcgcc ccagacgtca tggttgatcc cctctccggg
     7921 cgcaacgtcc ggccccttct tcagcctgcg gtagagcatg cgtcgtccgt ccggagaaaa
     7981 acgtccgcca aactcgctcc actcgggtgt gctggtcaga ttgcgtcggg ccgttccatc
     8041 gggacgcgag aggaagaggt cgtagtcgcc ctgtgcagtg cgcgcggcga acaccaggta
     8101 accaagacgg ccgacctcgt cggccagctt tcggctgtct ggatcgggcg actgggccga
     8161 gcccagaggc aggctcagga gtgcgcccgc caggagaagc cacccggttc ggagacggag
     8221 cttcggcgca cgcgccggga gggcgttgcc tgcgggggga cggctgggcg gatgatcccg
     8281 cccggagcga ttcgaggatg cgaattccat aacgatatcc tccggcttgt cgcacaccca
     8341 gccgcgcggc gcaacatgga acaagggcgc aacagggccg gcgtgacgtg tcggtgtcag
     8401 acgtcgtagt aacagatggc ccgctgcgta atcgaatcca cgttgagcaa aagcccagga
     8461 ttcgcgcgag gggcaggccg tcctcccaac gcccctggcc aggtcggggc ggccggcgcg
     8521 ggccggccgg gggcggacgt gcgtcaatgg actgcgccat gccgatatcg cgaatgcctt
     8581 tgcctttgcc gcacggatgc agaggcgcct tccggtgtca gattgccgtc gaggcgtcga
     8641 gcgaaatccc ttttgtatta tatcgactta tacttttcat actgatgtag aaaagtgtgg
     8701 accgccgggc ggctctccac taccagtaac tcggttcgta cttttatgcc gattcgtcga
     8761 tacggctcaa gaagcggttg cgcaaaccgc aaccgggagc gtttgaggcg attcgggcga
     8821 gacgcggctc aggccggctg acctctactt ctttctgttt tgttatggga ctcatccaag
     8881 acaccctcga tgtcgtgcgc aagcgcgacg cgaacgaacc ggagtttctt caggcggtga
     8941 cggaagtgtt cgagtcgatc gagccggcga tccagcgtca caagaagtac cgggatgcca
     9001 acattcttct gcggatcgtc gagccggagc gcgtggtgat gttccgcgtg ccgtggcagg
     9061 acgacaaggg ccagttccga gtcaaccgag gttatcggat tcagatgaac agcgcgatcg
     9121 ggccctacaa gggcgggttg cgctttcatc ccacggtgaa cctgggcatc ctgaagtttc
     9181 tcgcattcga gcaggtgttt aagaactcgc tgacgacgct gccgatgggt ggaggcaaag
     9241 gcggttcgga tttcgatccc aaaggccggt cggaccacga ggtgatgcgt ttctgccaga
     9301 gtttcatgat cgagttgtgc cggcatatcg gggcggacac cgacgtgccg gcgggcgaca
     9361 tcggggtggg ggggcgcgag atcggctatc tctacggtca atacaagcgc atcgccaacg
     9421 agttcaccgg cgtcctcacg ggcaagtccg tgtcgtgggg agggtcgttg atccgtcccg
     9481 aggccaccgg ctacggctgc gtctacttcg ccctcgagat gctcaagacc cgaggcgact
     9541 cgctcgaagg gcggatttgc gccgtgtccg gctccggcaa tgccgcgcag tacacggtcg
     9601 agaagctgat ccaggtcggg gccaaacccg taacgatgtc cgattccaac gggttcatct
     9661 atgataagga ggggatcgac gccgagcgtc tcgagtggat caaggacctg aagaatgttc
     9721 ggcgcggtcg catcaaggaa tacgctgaga agttcggcgc caagtacgtt gaaggtcaac
     9781 gcccctggct ggtcgactgc cactgcgcct ttccgtgcgc cacgcaaaac gaaatcagcg
     9841 gcgaggaagc caaccgcctc atctcaggcg gctgctttct ggtgtccgaa gcctcgaaca
     9901 tgccctccga gccggaggcg attcatctgt tcaccaacaa gaagatcctg tacggaccgg
     9961 ggaaggccgc gaacgccggc ggcgttgcca catcggggct cgaaatgacc cagaacgcca
    10021 tgcgcctgcc ctggtcgcgc gaggaagtcg accggcggtt gcgcgacatc atggccacca
    10081 tccacaagaa tgcctgggag accgcgatcg aatacgatcg tccgggcaac ctcgtcgtgg
    10141 gagccaacat cgtcgggttc gtcaaggttg ccgacgccat gctcgaacag ggcctggtct
    10201 gagtgagcgc ctcgggcatg tccgccatcg acggcctcca gcgtccttcg aggccttggc
    10261 ttcgatcggt gccgggaggc gcactccccg cccggttgcc gtccttcttg ctgctcttgt
    10321 tgggatgggc gattctcacc ggcggatgcg cgtcgaagcc cgcgccgccg ctcgaacgcc
    10381 acgagttcag cgaaccgcag atgggcatcc cgttccggat cgtgctctat gcgcccaatc
    10441 ccgcgtctgc ccgcgcgtcg gcccgtgccg ccttcgatcg gattgcggcg ttgaacctcg
    10501 tcctcagcga ttacgaggac gagagcgagt tgacgcgact gaaccgcacg gctgggagcg
    10561 ggcgggcggt tgcggtgagc gacgatctat ggcgggtcct ctcgcgcgcc caagagatgg
    10621 cgcggcgcac tggaggtgct tttgacgtga cagtcggccc ctgcgttcag ttgtggcggc
    10681 gggcacgacg ccagcgggcg tggccggagg catcgcgact cgaggccgcg cgcggggcgg
    10741 tcggctttca gcatctcgaa ctcgatccac tgcggcgaac cgctctcctg cggcggccgc
    10801 ggatgcggtt ggacgtggga ggaattggga aagggtatgc gctgcaggaa gcgctcgaaa
    10861 cactcgaagc gcgaggcata cgccgcgccc tggtgatcgg cggcggcgac atggtggccg
    10921 gcgatcctcc gcccggcaag acgggctggc aaatcgagat tgcgccgctc gatgcgacca
    10981 atgcgccgcc agcccagttc gtcctgttgc ggcggcaggc cctggcgacc tcgggcgatc
    11041 ttttccagcg cttcgagttc ggcgggcggc gctattcaca catcgtcaac ccccgcacag
    11101 ggattgggct gacggaccac agcctggtga cggtgattgc gagggacggg atgacggcgg
    11161 atgcgctctc gaccgcggtg agcgtgctcg gcccggatgc ggggatgcgc ctggtcgatc
    11221 gggagcgcgg cgccgcggcg cacgtggtgc gcgaggtcga cgggattctc gaacggcgtg
    11281 tgagccgccg ttttcgggcg gggaggaatg cgaggggaga atgcgcttgc gccggtcccg
    11341 ctcgttggta gagtcagccc cgctttaaca ggcccgaggc caccggcagc ggtggttttt
    11401 ttattgtgcg aactcggaca ggacgatcgt gaagattttc agcggcacat ccaatcagcc
    11461 tctggcggag gcgatctgca agtacatcgg cattgaactg ggcaaatgcg atgtgacccg
    11521 gtttccggat ggcgagacgt ttgtcaagat cctcgagaat gtccggggcc aggacgcgtt
    11581 catcgtgcag tcgacgagtc cgcccacgaa ccatcacctg atggagttgt tcatcatgat
    11641 ggatgccttg cgccgggcca gcgccagccg gatcacggcg g
//
LOCUS       DAOQRO010000068        11537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4767_length_11537_cov_6.447744, whole genome shotgun sequence.
ACCESSION   DAOQRO010000068 DAOQRO010000000
VERSION     DAOQRO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4767_length_11537_cov_6.447744"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1814)
                     /locus_tag="PKK20_04020"
     CDS             complement(195..1814)
                     /locus_tag="PKK20_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99084.1"
                     /translation="MNHRTLATIAVLSFALCGCPCRSALGGEGKTRSLFMPRELRERA
                     WANMRQDETMTRLRDQIVERARPWMELSDDQVWGLMFGPGITRSWMVWSDGHCPACGK
                     SVPMYNWRMDALNRPWKTWCPHCAAVFPANDFEAFYRSGLDRRGIFDPALADRSLLFN
                     AAHPDPNDPRHRFGVDDGEGFVQDGKRWRFIGAYLIHGQWKQAVLGGINALSEAYVVT
                     GDKAYARKAGLLLDRVADVYAGFDFAQQAVIYERRMESAGYVSVWHDACEETRELAQG
                     YDAVFDAIAEDADLVALLRAKAAQHGLENRKESFADIRRNIEDRILRDALDHISKITS
                     NYPRTPIAIATIKAVLDWSGSRDEVYAIIDDFLRRATSVDGVTGEKGLAGYSAFGLQS
                     TALFLAMWDRAIPGFLEEMLDRHPKLHDMYRFHVDTWCFTRYYPLVGDTGWFATRTDQ
                     YQGVRFQRFGDASVSSRRDPVLAPSMFTFMGRLHELTRNPAFIQVLYLANDRAVAGRW
                     RSRGVYRVCSREVDHLTARGLAFQGGGGTGA"
     gene            complement(1892..2224)
                     /locus_tag="PKK20_04025"
     CDS             complement(1892..2224)
                     /locus_tag="PKK20_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99085.1"
                     /translation="MECVVDKSSLCAALKGSKIPRREDFGELRWAAREENAGYGTAFL
                     DTATGRRLWTGHDLDCDHEVDAAPACPPILIAGNVVVHVDSSAFIIRTENYLYRVEET
                     ISMSRRAI"
     gene            complement(2237..3976)
                     /locus_tag="PKK20_04030"
     CDS             complement(2237..3976)
                     /locus_tag="PKK20_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99086.1"
                     /translation="MRRLSFLLGMGWAGAACAWAQATFTLSVSPALPSNAYIVEVAQR
                     AGFFFAEEPPGQAGRNRLLVLAGEHHLTDDQRKQITAFVENGGALVSLGGLHGMPELF
                     GAELVRAGGAKGFGASAVLQLGEGYLDVRGSPHPVFAGLRSSLHFFGGLAVRATEAEA
                     TGTVLDRHRRSKPFAVLLERRVGRGLALLIAPDLLNSIVHIQQGIAVLRDGTPAFDGT
                     MPINDRILKTDDGQVLDDDFDRVVPAEGEAPAFLNPVADELRLVILRAIQYAARAVGQ
                     PLLQIAPWPREVPAVGCVSHDSDGNIEAAARAMLQEVKQAGIRTTWCTLFPSGYPRSV
                     YDEIRAAGCEIALHYDAMKESPDRQWGFDKLVRQFKGLITESGLTNIIANKNHYTRWE
                     GRLDFFRWCEQVGLKIDGTMGPSKRGGCGFPRGTCQPWRPLDDEAAPPRFLDVYEIPL
                     IAQDLEIHLPAKLAPWMLDQAVAHGGVAHFLFHPAHIQKPGLADALQTLVREGRQRGM
                     EWWTHAEVWEWETARRGVRLTEMAAVGTKSRVTVSSGKPLKDATLIVHEPDGNQRRLT
                     FDLLESKAVEIDL"
     gene            4612..5439
                     /locus_tag="PKK20_04035"
     CDS             4612..5439
                     /locus_tag="PKK20_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNU99087.1"
                     /translation="MPSQVIAVANQKGGVGKTTTTVNLAACLAAMGQRVLLIDLDPQA
                     NATSGLGLPKTEGGSAYRALLGEEPLSGRIKTTGCDRLELLPSELDLCGADVELPRVE
                     NHLQRLALALEPVRRDAGRDLILIDCPPSLGILTLNAFVAADYVLVPLQCEYYALEGI
                     TMMKRLLDQLRASGANPKLQLLGVLMTMFDIRTRLSQQVVSEVREHFKEAVFDTVIPR
                     STRLAEAPSFGKPIIQYDPYGAGAAAYEVLAQEVVARLGRAPSDAPAPSPDISKGCA"
     gene            complement(5654..7033)
                     /locus_tag="PKK20_04040"
     CDS             complement(5654..7033)
                     /locus_tag="PKK20_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99088.1"
                     /translation="MKRLTCSIVPVLLLLTALGQPAEPAVRIATFDVDATPPLGSAMA
                     YDPVKRLDDLSLRCRGIVLTGAEQPIVLCAVDWIAIANEGHEAFRAELAEAAGTVPSR
                     VAVHAVHQHDAPGCDFTAERIIGELGVNGYSRFDGAFHRQVIRRASDAIRAAMPNARA
                     ITHYGYGEAVVVEVASQRRIKGPDGRIRASRTSSTRDAAIRAEPEGVIDPVVSVLSFW
                     DGASPVAVLSFYACHPQSYYRLGIPTPDFPGIARFMRAQDVNAALHVHFNGAGGNVTA
                     GKYNDGAQENRMLLAKRLASGMKRAFEKSIKARLSAQDIGWSSIFVDLPLAPHLDEAK
                     LIAELKTTPPRGYISRADQLAWVRRVQAGHRIDVTCLRVGNTRMLHLPGELFVEYQLA
                     AKAMRPDLRVMMAAYGDYGPCYIGTALAYLEGGYETQPTSSNTTPEAEAVLMAAMRRL
                     LGQANRNEE"
     gene            complement(7508..9229)
                     /locus_tag="PKK20_04045"
     CDS             complement(7508..9229)
                     /locus_tag="PKK20_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNU99089.1"
                     /translation="MILAAFTLTATALAGPADQPSPAAPAIETPTPRPYPERLRWWAE
                     ARFGIFVHWGPVSLKGTEISWSRANTNPKCPNRGPIPADVYDNLYKEFNPTNFDAGRW
                     IGQARAAGAGYIVLTAKHCDGFLLWHSKASDYNIGSTPFRRDVCAELAKAARAQGVRL
                     GWYFSPMDWRDPDFRSDRQAAFLDRMQAEVRELLSDYGRIDLLWFDWDGGDPLYDQAR
                     TYGLVRQLQPSIIINNRLDLGPGNSDRQILSVQADYYTPEQHVGAYDDQRPWESCMTL
                     SSRNQWAWGGPTDGVKPFAACLAMLIGCAGGDGNLLLNVGPMPNGEIPSDQAARLKEL
                     GAWLAQYGESIRGTRGGPFKPGGYGASTRKANTIYLHIREWDEESLRLPPIAARVVSS
                     RVLTGGTVAVKQTANGLDISVPAGGRHPLDTIVALELDGDALRLPALAVPSPRSLTAR
                     AKATASNVFQGQAAYGPDKAVDGSDETRWATDAGVKSAWLEVDLGRPLTFRRALVKQA
                     YPELDRVRKFAIEYFDGGEWKACYRGEALGARLNARFEPVTAQRVRLNLLETTDGPTI
                     FEFALFE"
     gene            complement(9320..>11537)
                     /locus_tag="PKK20_04050"
     CDS             complement(9320..>11537)
                     /locus_tag="PKK20_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HNU99090.1"
                     /translation="PGYLMPICDLEAGAGGDALAQFAIDFSNRINAVLRIRPGIYING
                     NYSSILQGATAARRDLLAKPVAYTPSVTGPAFPMLWNARYSDNGNPSAIPVQTGSPKH
                     TYTTYAGYYGPWDDYGNADPWSFWQYASTVSIPGFAAVDGTVDGNVAHGDIEDVRNSL
                     VPAVWWSDRSGDWSVLTNWNSGQPVTAPVTPPDQATPYATGPLPVPRLPGAPGSGPTS
                     GQHDTVIIERPAASVTVTVSAGIHIIRKLYLREALNLTGGSLTINYNPHYRADDSPHV
                     RHAGPVSAQFSGPVSIGGNARLAVHTLQVDASRVLTLAGGTLAFETIRLMPHATAPAR
                     ILVTGNASVNPWSDATAVIARGAGAGTSGYIDLGGDMREIAVGDGSSEVDLSIDVSLA
                     NGGLSKTGPGTLRLASASTYAGGTTVSGGRLLVTNPAGSGTGVGGVTVVGGTLGGTGV
                     IAGAVTIDREGTLAPGVSIGTLTLQSPPALGGVLMLEIDRNGGAPVADRIVLTTGTLD
                     FGGTLVVTNVGAVLTGGEMFRLFVAPACAGAFADWRLPPLGAGLNWFMGALVADGTIA
                     VNRAPVPGDLPTLTNVAPALLEIPLALLTGSATDADGDALFVSGVASASTNGIPLGIH
                     GEIIVYANDASVADEFNYTLSDGRGGSATGVVRIVNIGAKPPAVFVGPPSVSGGAVLL
                     RFSASPGRTYDLERSTDLVHWRTIWTGVAPAGAMLEYTDEFRDLHAPPAAAFYRVR"
BASE COUNT         2051 a         3846 c         3654 g         1986 t
ORIGIN      
        1 gattcctgtc cggatcaaat cggaatcggt gtccgaatgc cgccggaata cgcaccaatc
       61 ttcgacaaac cgaaaactgg tcgcgatctg gattgccaaa aacccaggtt ctggctatgg
      121 acaccgacga cgtttaagcg ccccttgtct tacagaaacg gccgctggct gtcgaggtag
      181 ggcaaggggt ttccttacgc cccggtcccg ccccctccct ggaaagccaa gcccctcgcc
      241 gtcaagtggt caacttcccg tgagcagacc cggtaaactc cccgtgagcg ccaacgcccc
      301 gcgacggcgc ggtcgttagc caggtacagc acttggataa aggcgggatt ccgggtcagt
      361 tcgtgcagtc gccccatgaa ggtgaacatc gacggggcca gcaccggatc cctgcggctg
      421 gaaacgctcg catccccgaa gcgctgaaat cgcacgccct ggtattggtc ggtgcgcgtg
      481 gcgaaccagc cggtgtcgcc caccagcgga tagtaccggg tgaagcacca cgtgtccacg
      541 tggaagcgat acatgtcatg aagtttggga tggcgatcga gcatttcttc caggaatccc
      601 gggatcgccc ggtcccacat ggccaggaac agggccgtgc tctgcaagcc aaaggcggag
      661 taacccgcca aacccttctc ccccgtgacg ccatccaccg acgtggcccg ccggagaaag
      721 tcgtcgatga tcgcatagac ttcgtcccgg ctgcccgacc agtcgagaac cgctttgatc
      781 gtggcgatgg cgatgggcgt gcgcgggtag ttggacgtga tcttcgagat gtggtccagc
      841 gcgtcgcgca gaatccggtc ctcgatattg cggcggatgt ccgcgaacga ttccttgcgg
      901 ttctcgagac catgctgcgc cgctttggcc cgcagcagcg ccaccaggtc agcgtcttcc
      961 gcgatcgcgt cgaagaccgc gtcgtagccc tgcgccagtt cccgggtctc ctcgcaggcg
     1021 tcgtgccata ccgaaacgta acccgcgctc tccattcgcc gctcgtagat caccgcctgt
     1081 tgcgcgaaat cgaagcccgc gtacacgtcc gccacccgat ccagcaggag gccggccttg
     1141 cgggcgtacg ctttgtcgcc cgtcacgacg taggcttcgc tcagcgcgtt gatgccgccg
     1201 agcaccgctt gcttccactg gccgtggatc aggtaggcgc cgatgaaccg ccaccgcttg
     1261 ccgtcctgca cgaacccctc tccgtcgtcc acgccaaacc ggtgccgcgg gtcgttgggg
     1321 tcggggtgcg cggcgttgaa cagcagcgag cgatcggcca aggcagggtc gaagatgccg
     1381 cgccggtcga gccccgaacg gtaaaatgcc tcgaagtcat tggccggaaa caccgccgcg
     1441 cagtggggac accaggtctt ccacgggcgg ttgagggcat ccatccgcca gttgtacatg
     1501 ggcacgctct tgccgcaggc ggggcagtga ccgtcggacc agaccatcca ggaacgtgtg
     1561 atgcccggcc cgaacatcag tccccagacc tgatcgtcgg acaactccat ccaaggacgg
     1621 gcccgctcga cgatctggtc tcgcaggcgc gtcatcgttt cgtcctgacg catgttcgcc
     1681 caggcccgct cacgaagctc gcgcggcatg aacaggctgc gcgttttccc ttccccgccc
     1741 aacgcgctgc gacacgggca cccgcacagg gcgaacgaca gaacggcgat cgttgcaaga
     1801 gtccggtggt tcatgcagtc ggttcccttc ggatcgagat ggtcttcgaa tttttgcgag
     1861 gtcttcccga gattggccgt aatgttggcg atcagattgc tctccgtgac atgctgatgg
     1921 tctcctcgac ccggtacagg tagttctctg tgcggatgat gaatgcgctg ctatcgacgt
     1981 ggaccacgac gttgcccgcg atcagaatgg ggggacacgc cggggcggcg tcgacttcat
     2041 gatcacaatc gaggtcgtgg ccagtccaga gtctgcggcc ggtcgcggtg tccaggaagg
     2101 cggttccata tccggcgttc tcctcccgcg cggcccacct gagctcgccg aagtcctcgc
     2161 gccttggaat ctttgacccc ttcaacgcgg cgcagagact tgatttgtct acaacacatt
     2221 ccaccggtca aggattctac aagtcgattt ccacggcctt cgactccagc aagtcaaagg
     2281 tcaaccgtcg ttggttgccg tccggctcat gcacaatcag agtcgcatcc ttcagcggtt
     2341 ttccactcga caccgtcacg cgcgacttcg ttccgaccgc tgccatttcc gtcagccgca
     2401 cgccgcgacg cgcggtctcc cactcccaga cttcggcgtg agtccaccat tccatgccgc
     2461 gttgacggcc ctcccgcacc agtgtctgca gcgcgtcagc caggccgggc ttctggatgt
     2521 gcgcgggatg aaagagaaaa tgcgccaccc cgccatgcgc caccgcctgg tccagcatcc
     2581 agggcgccag tttcgccggc aggtggattt ccaggtcctg ggcaatcaac gggatctcat
     2641 aaacgtccag aaaccgcggc ggcgcggcct cgtcgtccaa aggccgccac ggctgacacg
     2701 tgccgcgcgg aaagccgcaa ccgccacgct tgctcggccc catcgtgccg tcgattttca
     2761 gcccgacctg ctcgcaccag cgaaagaaat cgagccgtcc ttcccatcgc gtgtaatggt
     2821 tcttgttcgc gatgatgttc gtgaggccgc tttcggttat cagtcccttg aactgtctga
     2881 ccagtttgtc gaagccccac tggcgatccg gactctcttt catcgcgtcg tagtgcaggg
     2941 cgatttcgca gcccgcggcg cgaatctcgt cgtagaccga gcgcggatac ccgctgggga
     3001 acagcgtgca ccaggtcgtg cggatgcctg cctgcttcac ctcttgcagc atggcccgcg
     3061 cggccgcctc gatgttgcca tcgctgtcgt ggctgacgca tcccaccgcc ggcacctcgc
     3121 gcggccacgg ggcgatctgg aggagtggct ggccgacggc gcgcgcggcg tattgaatcg
     3181 cgcgcaaaat caccagccgc agctcgtccg ccaccgggtt caggaacgcc ggcgcctcgc
     3241 cttcggccgg caccacgcga tcgaaatcgt cgtcgagcac ctggccatcg tccgtcttca
     3301 ggatgcgatc gttgattggc atggttccat cgaacgccgg cgtgccgtcc cgcagcacgg
     3361 cgatgccttg ctggatgtgg acgatactgt tgaggagatc cggcgcgatc agcaacgcca
     3421 gaccgcggcc aacgcgtcgc tccaacagaa ccgcgaaggg cttcgaccgg cgatgacggt
     3481 ccagcaccgt gcccgtggcc tcggcttccg tcgcccgcac cgccagccct ccaaagaaat
     3541 gcagcgagct cctcagcccg gcaaacaccg gatgcggcga accccggaca tcaaggtaac
     3601 cttcgcccag ttggaggacc gcgctcgccc cgaagccttt tgcgccgccc gcccgcacca
     3661 actccgctcc gaacagttcc ggcattccgt gcaacccgcc cagcgacacc aaagcgccgc
     3721 cgttttcgac gaacgccgtg atctgcttgc gctggtcgtc ggtgagatgg tgctcgccgg
     3781 cgagcaccag caggcgattg cggcccgctt ggcccggcgg ttcctcagcg aaaaagaagc
     3841 cggcgcgctg ggccacttcg acgatgtatg cattcgacgg caatgcggga gacacagaga
     3901 gggtgaaagt cgcctgcgcc caagcgcacg cggcgccggc ccaccccatt cccagcagaa
     3961 agctcagccg tctcattctg ctacaactcg gcaaggctgt gccccagact acaatgcggc
     4021 agttcacatt cttccgattc gcggagcata cccatgccac ggcgatcagg acaaacaggc
     4081 cgatgaaact caccaggttc tgcatgctct ttccctatcc aaatcgcccc tcaaacacca
     4141 gctccccgat gtgctttctc tcattcgacc caattccgac cggcggcatc tgaacggtgc
     4201 caacgggcct caccaacgtc gttgcgattg cctgcgtcgg cttccacaac ttcgcgcaga
     4261 tgcgcgaagg ctcgtcacac tgctgcgcaa gcaactggcc gaccatccgg tcacattccc
     4321 gacggccaaa gcccccgcaa cctaccccat gatggtcggc agcgccgccg cctgagattc
     4381 tcccctcgcc gcctcacact gccccccgcc aaaatccgtc ttcagaatgt ccaaactcca
     4441 gacttcgcgc aggtgcgcga aggcgatccg gctccttgac cttaccgacc aaggcatgaa
     4501 gggggcgtga aagggtcggt gccggaagtc ggtttgatcc tgatcgtgga agccgtcact
     4561 catcgaagat tcgcgtttga attcggggcg tctgttgcct ttaatccccg cgtgccaagt
     4621 caagtcatcg ccgtcgcgaa ccagaaaggg ggcgtcggca agaccaccac gaccgtcaac
     4681 ctggcggcat gcctggccgc catggggcag cgtgttctgc tgatcgatct cgacccgcag
     4741 gccaacgcca caagcggtct gggcctgccc aaaaccgagg gtggcagcgc ctaccgcgcc
     4801 ttgctcggag aggaaccgct gtccggacgc atcaagacca cgggctgcga tcgcctcgaa
     4861 cttctcccca gcgaactgga cttgtgcggc gcggacgtcg aattgccccg cgtggaaaat
     4921 catctccagc gcctggcgct cgccctcgag ccggtgcggc gcgacgcggg gcgcgacctg
     4981 atcctgatcg attgcccccc gtcactgggc attctgaccc tcaacgcctt cgtggcggcc
     5041 gattacgtcc tggtcccgct tcagtgcgaa tactacgccc tcgaggggat cacgatgatg
     5101 aagcgtctgc tggatcagtt gcgggcgtca ggtgccaatc ccaagctgca gttgctcggc
     5161 gtgctgatga cgatgttcga catccggacg cgcctctccc agcaggtcgt gtcggaggtc
     5221 cgtgaacact tcaaagaggc cgtgttcgac actgtgatcc cgcgcagcac gcgtctggcc
     5281 gaggccccga gcttcggcaa gccaatcatt cagtacgatc cctacggcgc cggcgccgcc
     5341 gcctacgagg tcctggccca ggaagtcgtg gccaggctcg gacgcgcacc gtctgatgcc
     5401 cctgccccca gcccggacat ctcaaaaggc tgcgcgtgat cggaatcccg ttctcggtgc
     5461 tctctgcgtc tctgtggtca atccatcaca atgaccgcca cgggagggat gaaccacaga
     5521 gatacgaaag gcgcggagaa gacatgccgg ccatcgcgct ccccgacggc agcattgcaa
     5581 gcccgttcct gactctgttc ttaagaatgc accatcccat tcaccttcaa tccgatgcgc
     5641 aacctcgctc cggctattcc tcattgcgat tcgcttgtcc gagcaatctg cgcatcgcgg
     5701 ccatgagcac ggcttcggcc tccggtgtgg tgttcgacga ggtcggctgg gtttcgtagc
     5761 cgccttcgag gtaggcgagg gcggtgccga tgtagcaggg gccgtaatcc ccgtaagcag
     5821 ccatcatgac acgcaggtcg gggcgcatgg ccttggccgc aagctggtac tcgacaaaca
     5881 actcgcccgg caggtgcagc attcgcgtgt tgcccacccg caggcaggtg acgtcaatcc
     5941 gatgtccggc ctgcacgcgt cggacccagg cgagttggtc ggcacgggaa atgtagcctc
     6001 tcggtggcgt ggtcttgagc tcggcgatca gcttcgcctc gtccagatgc ggcgcgagcg
     6061 gcaggtcgac aaagatactg ctccaaccga tgtcctgggc ggacagccgg gctttgatcg
     6121 acttttcgaa ggccctcttc atccctgacg ccagccgctt ggccaggagc atgcggttct
     6181 cctgggcgcc gtcgttgtac ttgccggccg tcacattgcc gccggccccg ttgaaatgca
     6241 cgtgcagggc ggcgttgaca tcctgggcgc gcatgaaccg ggcgatgccg gggaagtcgg
     6301 gcgtgggaat gcccagtcgg taatagctct gcggatgaca ggcatagaag ctcagcacag
     6361 ccacaggcga tgccccatcc cagaaactca ggaccgacac cacaggatcg atcacaccct
     6421 cgggctcggc ccggattgcg gcatccctgg tggaactggt gcgcgatgcg cgaatgcgcc
     6481 cgtccggccc cttgatgcgg cgctggctgg caacttccac aaccaccgcc tctccgtatc
     6541 cgtaatgggt gatcgctcgg gcgttgggca tcgccgcgcg gatcgcatcg gacgccctcc
     6601 ggatcacctg gcgatggaag gcgccgtcga aacgcgagta gccgttcacg ccgagttccc
     6661 caatgatcct ctcggccgtg aaatcgcacc cgggcgcatc gtgctgatgc accgcatgca
     6721 cggccacgcg cgacggcacg gtcccggccg cttcggcgag ttcggcgcga aaagcctcgt
     6781 gcccctcgtt ggcgatcgcg atccaatcca ccgcgcagag cacgatcggc tgctcggcgc
     6841 ccgtcagaac tatcccgcgg cagcggagac tcaggtcgtc gaggcgcttg acgggatcgt
     6901 aggccatggc cgacccgagc ggcggcgtag cgtcgacgtc aaaggtggcg atgcggaccg
     6961 cgggctcagc cggctgaccg agagcggtca acagcagaag aaccgggaca atcgagcaag
     7021 tcaggcgttt catggtacgc aggctaaggg tctgtgcaaa aattacttcc ggttttggcg
     7081 ggagcgccac cggaccagat gcgaggcccg agggagggag tgtatcccca gagatactcg
     7141 accgaccgag gaagtgtgaa cgctgcgggc cgcctgcggc gcttcgatgc gcttcttggc
     7201 gaaaaacaac gcatgtccag gcggttcgaa tctccgatga tcttcaagcc tgggtcatca
     7261 tgctggcccg caagcgaaga gtcaatcgtc ggaggcgaat gcaagagcgc ctccagcgcc
     7321 aatgcatgaa gtcgaaaagg tcgagggtgg ggacggctcg tcccccgcca tgccaagtga
     7381 aaacggcgtt tgtcggcgac ggggccctgc ctgccctgcc agccctgccc tgcctgccgg
     7441 caggcaggcg gcaggcaggc ggcaggcagg cgtcgccgct ccacttcatg aattggccct
     7501 cgaacgccta ctcgaagagc gcgaactcga agatggtcgg gccgtcggtg gtctcgagga
     7561 gattcaggcg cacgcgttgc gccgtgaccg gctcgaaccg cgcattgagc cgggcaccga
     7621 gtgcctcacc ccggtagcag gctttccatt cgcctccatc gaaatactcg atcgcgaatt
     7681 tccggacccg atcgagttcc ggatacgcct gcttgacgag cgcgcggcgg aaggtcaggg
     7741 gccggccgag gtccacttcg agccaggcgg acttgacccc cgcatcggtc gcccagcggg
     7801 tctcatcgga cccgtcgacg gccttgtccg gaccgtacgc cgcctgtcct tgaaaaacat
     7861 tcgacgcggt tgctttggcc cgtgcggtca gcgatcgagg gctaggcacg gccaaagcgg
     7921 ggaggcgcaa ggcgtcgcca tcgagttcga gcgccacgat ggtgtcgagc ggatggcggc
     7981 cgcctgccgg cactgagatg tcaagaccgt tggcggtctg tttgacggcc accgtgccgc
     8041 cggtcagaac gcggctcgag acgacccgag cggcgatggg cggcagccgc aggctctctt
     8101 cgtcccactc gcgaatgtgg aggtagatcg tgttggcctt gcgcgtcgaa gcgccgtaac
     8161 cgcccggctt gaaaggcccg ccgcgcgtcc cgcggatgct ctcgccgtac tgggccagcc
     8221 acgctcccaa ttccttgagc cgcgcggcct ggtccgaagg gatttcgcca ttgggcatgg
     8281 gaccgacgtt gagcaataga ttgccgtctc cgccggcgca tccgatcagc atggccaggc
     8341 acgcggcgaa cggcttcacg ccgtcggtcg gtccgcccca ggcccactgg ttgcgcgacg
     8401 aaagcgtcat gcacgattcc cagggccgct ggtcgtcgta ggcgccgaca tgctgctcgg
     8461 gggtgtaata gtcggcctgg accgacagaa tctggcggtc gctgttgccc gggcccagat
     8521 cgagacggtt gttgatgatg atcgagggct ggagctgccg cacaaggccg taggtgcgcg
     8581 cctggtcgta gagcgggtcg cccccgtccc agtcgaacca caaaagatcg atgcgcccgt
     8641 agtcgctcag gagttcgcgc acctcggcct gcatccggtc caggaatgcg gcctggcgat
     8701 cagatcggaa gtcggggtcc cgccagtcca tcggggagaa gtaccagccg aggcgcaccc
     8761 cctgcgcgcg ggccgccttc gccaactcgg cgcacacgtc gcggcggaag ggtgtggacc
     8821 caatattgta gtcgctcgcc ttcgagtgcc agagcaggaa accgtcgcag tgcttggcgg
     8881 tcaggacgat gtacccggcg ccggcggccc gggcctggcc aatccaccga cccgcgtcga
     8941 agttggtcgg attgaattcc ttgtagaggt tgtcatagac atcggccggg atcggcccgc
     9001 ggttgggaca cttcgggttc gtgttggccc gcgaccaact gatctcggtg cctttgaggc
     9061 tgaccggtcc ccaatgcaca aagatgccga accgcgcctc ggcccaccag cggagccgct
     9121 ccggataggg acgcggcgtc ggggtctcga tcgcaggcgc cgctggagat ggctggtcgg
     9181 ccggaccggc aagggcggtt gcggtgagcg tgaaagcggc caggatcatg ccaacgaggc
     9241 ggcctctcat gcaggtgaac atcgcgcaga ctctgatgcg aggaagcggc cgacggcaag
     9301 tcgcaagttg cggccaatcc tatcgcaccc gatagaacgc ggccgcgggc ggggcgtgca
     9361 ggtcgcgaaa ctcgtccgtg tattcaagca tagccccggc cggcgcgacg cccgtccaga
     9421 tcgttcgcca gtggaccagg tccgtcgaac gttcgaggtc ataagtccgc ccgggcgaag
     9481 ccgagaaccg gagcagaacg gcgccgccgc tgacgctggg cgggccaacg aacaccgcag
     9541 gcggcttcgc gccgatattc acgatgcgga ccacacctgt cgccgaaccg ccccggccat
     9601 cgctgagggt gtagttgaat tcgtcggcga cgctggcgtc attcgcgtag acgatgatct
     9661 ctccgtggat tcccagggga atcccgttgg tgctcgcgga tgcaacaccc gaaacgaaaa
     9721 gcgcatcccc gtccgcgtcg gtggcgctcc ccgtcaacag cgccaaggga atctccaaca
     9781 gcgccggggc cacattggtc agcgtcggca gatcgcccgg aaccggcgca cggttcacgg
     9841 cgatcgtccc gtcggcgacc aacgcgccca tgaaccagtt caggcccgcg cccagtggcg
     9901 gcagccgcca atccgcaaac gcgccggcgc aagccggggc gacgaagagc cgaaacatct
     9961 cgccgcctgt cagcaccgct cccacgttag ttaccaccag cgtcccgccg aaatcgagtg
    10021 tgccggtggt cagaacgatc ctgtccgcca caggcgcgcc gccattgcga tcgatttcca
    10081 gcatcagcac gccgcccagt gctgggggac tttgcagggt gagagtgcca atggacacgc
    10141 ccggagcgag tgtgccttcg cgatcgatgg tgacggcgcc ggcgatgacg cccgtgcccc
    10201 cgagcgtgcc gcccacgacg gtcacgcctc cgacgcccgt ccccgacccg gcggggttgg
    10261 tcaccagcaa ccggccgccc gacaccgtgg tcccgccggc gtacgtgctg gctgatgcaa
    10321 gacgcagcgt ccctgggccc gtcttcgaca ggccgccgtt ggcgagcgaa acatcgattg
    10381 agagatccac ttcactcgag ccgtctccca cggcgatctc gcgcatgtcg ccgccgaggt
    10441 cgatgtatcc ggaagtgccg gcgccggcgc ccctggcgat gacggcggtg gcatccgacc
    10501 aggggttcac gctcgcgttt ccggtcacca gaattctggc cggggccgtc gcgtgcggca
    10561 tcaggcgaat ggtctcgaag gcgagagtgc ccccggccag cgtcaagacg cggctcgcat
    10621 ccacctgcag tgtgtggacg gcgaggcgag cgttgccgcc gatcgagacc ggaccggaga
    10681 actgggctga gacgggtccg gcgtgccgga cgtgcggcga atcatcggcc cggtaatgcg
    10741 ggttgtagtt gatcgtcagc gagccgccgg tcagattgag cgcctcgcgc agatagagtt
    10801 tgcggatgat gtggattccg gcggagacgg tgacggtgac gcttgctgcg ggccgctcga
    10861 tgatgaccgt gtcatgctgg ccagaggtcg ggccgctgcc cggcgcgccc ggcagccgcg
    10921 gcacgggcaa cgggccggtg gcgtacggcg tcgcctggtc ggggggcgtg accggcgccg
    10981 tgaccggttg accgctgttc cagttggtga gcacgctcca atcgccgctg cggtcgctcc
    11041 accaaaccgc cggaacaaga gagttgcgga cgtcctcgat gtcgccgtgg gcgacgttgc
    11101 catccacggt cccatcgacc gcggcgaagc cgggaatcga gactgtgctg gcgtactgcc
    11161 agaaggacca cggatcggcg ttgccgtagt cgtcccacgg accgtagtac cccgcgtacg
    11221 ttgtgtaggt gtgcttgggg ctgcccgtct ggaccgggat cgcgctcggg ttgccgttgt
    11281 ccgaatagcg cgcgttccac agcatgggaa aggccgggcc ggtgacgctg ggggtatatg
    11341 cgaccggttt ggcgagcaag tcgcgacgcg ccgccgtcgc cccctggagg atgctcgagt
    11401 agtttccgtt gatgtagatg cccggtcgga tgcgcagaac cgcattgatc cggttggaaa
    11461 aatcgatggc gaactgagcc agcgcgtcgc cccccgcgcc cgcctcgagg tcgcagatcg
    11521 gcatcaaata cccgggc
//
LOCUS       DAOQRO010000069        11513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4784_length_11513_cov_4.512306, whole genome shotgun sequence.
ACCESSION   DAOQRO010000069 DAOQRO010000000
VERSION     DAOQRO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4784_length_11513_cov_4.512306"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKK20_04055"
     CDS             <1..328
                     /locus_tag="PKK20_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99091.1"
                     /translation="LREGEPEYSAPVSREFYWSAIERRIASPRTPQPQAPAAPPFAWL
                     ARWFVPAGILAALALVLMLPALRDQFAPPVKRASEEVESPLDDISSVTFRSESEGITV
                     VWVNAR"
     gene            399..869
                     /locus_tag="PKK20_04060"
     CDS             399..869
                     /locus_tag="PKK20_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99092.1"
                     /translation="MNRIILHTLLCVAASLWFLAGTPAVSGADLALRSQLLWGTDQEK
                     PRESTYKEVDPKVKGKLSRIFKWKHYFEINQQKVTLEANDTKRLKLSAKCEIDVRFLD
                     AHTLEIKLYGEGKWTKTIRQSVAALNRGELAVLAGDDKDKYGDAWFVVLSADRP"
     gene            complement(923..2551)
                     /gene="murJ"
                     /locus_tag="PKK20_04065"
     CDS             complement(923..2551)
                     /gene="murJ"
                     /locus_tag="PKK20_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNU99093.1"
                     /translation="MLKSSGAMGVATLISRVLGLVREQVYAGFMGDGVVASAFKLAYQ
                     APNLFRRLLGEGALTAAFIPIFKQKERTEGEGAMWESANAVLSGLILFASIVVGVCLA
                     IVSLVLAAGSLRFSSGGDAVFPPFPIPILGEDTRLVLRLLQVMLPYLLLVCVAALCMG
                     LLNARGYFFVPAMSATMLNVVLIAAVLLLAPRFGDELETQVFALALGVVAAGVAQLGF
                     QWPTLRKDGFRYRWIRPWNNPTVREVVRRMLPATVGVAAFQLNVLIIAFIAFYVDRTL
                     VASFDYAVRLMEFPQGVVGISFATYLLPTLSGLAAEKKYPEFRATLREGLGYLLFVNL
                     LAAALLLALAEPMVRLLFERGQFTASSTHRAAMALTGLAPGLVAFSSVNVLARAFYAL
                     GDTQTPMKISVACLVLNLVCSLWLIGPFRQLGLGMANSITAYVNVWLLMRALRKKMPK
                     LDFIPLRSVALRLLAIAAVTGGVAWSGQHLWGMRWGHATLPQKLGEVFVPMAAAGVVY
                     WGLAIALRVPQAKEVSRLVFGQLRKPRTREIPSD"
     gene            complement(2605..3411)
                     /locus_tag="PKK20_04070"
     CDS             complement(2605..3411)
                     /locus_tag="PKK20_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU99094.1"
                     /translation="MDSLILNCLGTGDGWPCADRSHSAFLYRFPQTAFLIDCGESVSR
                     TVKASGWSYDLIECAFLSHLHFDHLGGFFMLMQSFWLEGRRRALTVHMPEDGISPVKR
                     LMDAGCVFEDLLAFRLRFKALRGRRSVVVGGVKVTPCPTTHLDQLRASFGSKYAQRFE
                     SFGFLIEGHGLRVAHSADVGAVEDLSPLLSKPVNLLVCELAHLDVEPLAAYLKDKPVG
                     RVLFVHLARALRQDMQATRKRIRGALGRIPFRIAEDGDRLAVRAVGMEGG"
     gene            3784..4542
                     /locus_tag="PKK20_04075"
     CDS             3784..4542
                     /locus_tag="PKK20_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99095.1"
                     /translation="MSRLLRKNKNGAFTLIELLVVIAIIAILAGMLLPALAKAKAKAQ
                     RINCANNLKQVGIAFRLFATDNGDRFPMSVTTNEGGVSDWLGTSPNMLAVNTYRVFAA
                     LSNELATPKTVVCPSDSGRVAPSNFVGMVNLADFAKGAKNGGVSYFVNPDADESRPQV
                     ILAGDRNITNTTLNPSVGGGTAYNKQSPGLKWNLMDTTMKTTGWSTAMHNGAGNIGLS
                     DGSVQQVTGARLREQIKNSQEDHLLLFPYVPNKN"
     gene            complement(4597..5619)
                     /locus_tag="PKK20_04080"
     CDS             complement(4597..5619)
                     /locus_tag="PKK20_04080"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNU99096.1"
                     /translation="MGDRVLITGGAGFIGSHLAERLLAEGRSVTIVDDFSTGSRANLG
                     RVEGHPRLRVIEARVSQCASLADLAGEASLIYHLAAAVGVDLVVRSPIRTIETNIQET
                     EAILAAAGRHGVPLLLTSTSEVYGKSQNEEFREEDDLLIGPPNLGRWSYACSKLMDEF
                     LALAYMRERGLPVIIARVFNTVGPRQTGRYGMVLPRLIAAARAGQPLRLFGDGLQTRC
                     FAYVDDTVEALIRLGGCPGAWGGVFNVGSTEETTIRDLALLVKELLKSRSELEFVPYD
                     VAYAPGFEDMRRRRPSVDKLERLTGFRPSTPLVEIIRLTAGLTRIFAPRQCAGRSDVA
                     EDRHAG"
     gene            complement(5634..7553)
                     /gene="ilvB"
                     /locus_tag="PKK20_04085"
     CDS             complement(5634..7553)
                     /gene="ilvB"
                     /locus_tag="PKK20_04085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNU99097.1"
                     /translation="MNRHPKTTARNTPPSGDTSKTAPETAASLARTRTPVRPIGRDGA
                     GSTPKAAPESSAPLLGSQILVKCLEREGVEAIFAYPGGASMEIHQALCKSKNIRVYLP
                     RHEQGGAFAAEGYARATGRVGVCMATSGPGATNLVTGIADAYMDSVPIVAITGQVVRS
                     MIGKGAFQETDIYGMTLPVVKHSYLITSIGEIPRVVKEAFYIARSGRPGPVLIDLPKD
                     IQQQRAQPVFPSSIELRGYNPNRRADEAALDEIVALIEKAERPVVYAGGGIITSGASD
                     ELRRFVAATRIPVTTTLMGCGAFPEDDPLSLKWLGMHGTAYANWAVAEADLLLAFGVR
                     FDDRVTGKVDKFCEHGTIVHIDIDASEINKNRKVHLPVISDIRFALSELNARLAKRPL
                     QKRYEPWHRQIAEWKSKAPLCYHITEEVLKSQHVRDHMGGDFSQVILPQEAVALLCEL
                     TRGEAIITTGVGQHQMWAGQFYQFRHPRQFLTSAGLGSMGYGYPAALGAKVAFPDRQV
                     VDIDGDGSFLMNVQELATAHIEGIAAKALILNNQHLGMVMQWEDRFYQGNRGNTYLGD
                     PDNMREIYPDFVAIAKAFSVPCERVIYKRDLRAAMQRMLDSDQPYVLDVITPYTEHVM
                     PFIPAAKTVKEMIWQ"
     gene            complement(7776..8882)
                     /locus_tag="PKK20_04090"
     CDS             complement(7776..8882)
                     /locus_tag="PKK20_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99098.1"
                     /translation="MSEKPRKRYDMNSPEARAVVDEIVHGTFMKEGTMAAFPTCFPGA
                     STPIPADESHITALAVSGDGMIYGGTSGRAAHLFVGIFHGVTGIVFDRGAIEGADHCA
                     AVCCGKTRFAACVNGAGGGRVITGELQGLPFDLIQEWGFGRPPLRDLGPVNGERIVHA
                     VADGGGSIVGITTGALFTVDVDTGTIAIVGPVAGAGRIAVGSRGRVFGQDGPGDLWRY
                     DTASRTITRHALKLPEGVWDKSPMTWARDNRSGLLYTADGQGRLFALDEDRGFSPVLG
                     LTALAPVGPMAVTHDGRVFGFCGSEMARMFCYNPAARAVSDLGVAVSVLERRRYGYLF
                     GDAVVGRDGQIVFGEDDDLGHLWLYFPRIERLKD"
     gene            complement(8890..10251)
                     /locus_tag="PKK20_04095"
     CDS             complement(8890..10251)
                     /locus_tag="PKK20_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNU99099.1"
                     /translation="MAGKLKRRDFLKGGALAAGTWLALPGMAGKLGTGEHTGADGEPR
                     PYRNSWYPFTGHPDADTCWSLSTGPDGRIYAAACAESVPGGVVKVARYNEEKDTLDYL
                     FALDEMVEDPSDSGRATQCKIHYSFAPSMADGVLYMATHLSGPPIDQPAYSPWRSWHD
                     PERCFRGSALLAFDTRKDEVLWWDTMIPKEGCRCLLHDEERHLLYALSYPRDHFIIYD
                     TRTRARRDAGRIGSINAQALFLDRKHRVWTTDDYGHLVRYDPETDRLERSPFVLPHNP
                     QFQTGWHSVFYDVAAAPDGESVFAVTWIAHPRLMRVWPNEGPWPRVEDLGPATQDRDS
                     SIPVDTFIDHCGGLVFAGDGQLYYVASRWRDPVYQPKLESGHPREGVVWRLNPKTLER
                     TEVAILEHPTHPAQYVSRGAVDHNGNLFFGVVGTKPVGIFQVHLPASRRKENAHLPIR
                     IWG"
     gene            complement(10624..10857)
                     /locus_tag="PKK20_04100"
     CDS             complement(10624..10857)
                     /locus_tag="PKK20_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99100.1"
                     /translation="MTRSQIESAIESGAAFTLRMADGREYAVPHRDYISISPKGTFVT
                     VYDDDERFVVLPLLTMTGLASIAPKHNGGASKE"
     gene            11212..>11513
                     /locus_tag="PKK20_04105"
     CDS             11212..>11513
                     /locus_tag="PKK20_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNU99101.1"
                     /translation="MDLKRTKALVTLLIALGAANAGPGVNATASSSTPLAKPNIIVIM
                     ADDLGYGDVSCYGATAVQTPAIDRLAREGVRFTSGYCSASTCTPSRARRSMAGVWTA"
BASE COUNT         2339 a         3996 c         3195 g         1983 t
ORIGIN      
        1 cctccgcgag ggcgagcccg aatacagtgc cccggtcagc cgcgagttct attggtccgc
       61 catcgaacgt cggatcgcca gcccccgaac cccgcagccc caagccccgg ccgctccgcc
      121 cttcgcctgg cttgcccggt ggttcgtccc ggccggcatc ctcgccgccc tcgccctggt
      181 cctgatgctg cccgcattgc gcgaccaatt cgcgcccccg gtcaaaaggg cttccgagga
      241 agtcgaaagc ccgctcgacg acatcagctc cgtcaccttc cgctccgaat cggaagggat
      301 cacggtggtc tgggtcaacg cccgctgatc gctcccatga tctcgtcatt ttcatcggtc
      361 attcatggga cgccattccc ttgggcatca agacaggcat gaacagaatc attctccaca
      421 ccctcctctg tgtcgccgcc tccctctggt ttctcgccgg cacccccgcg gtgtcgggtg
      481 cggacctggc cctcaggagc cagttgctct ggggcacaga ccaggagaaa ccccgggaat
      541 ccacctataa ggaagtcgat ccaaaggtca aagggaaact cagccgcatc ttcaagtgga
      601 aacactactt cgagatcaat cagcagaagg tcaccctcga ggccaacgac acaaaacggc
      661 ttaaactcag cgccaagtgc gagatcgatg tccgattcct cgacgcacac acactcgaaa
      721 tcaagctcta cggcgagggg aaatggacca agaccatccg ccagtcggtc gccgccctga
      781 accggggcga actcgccgtt ctggcaggcg atgacaagga caaatacggc gacgcctggt
      841 tcgttgtcct gtcggcggac agaccctgac agctcatcca aaccgtgctc gaaaccgcct
      901 gacccccggc tcatccccga ggtcagtccg acggaatctc ccgcgtgcgc ggctttcgca
      961 gttgtccgaa caccaggcgg ctgacttcct tcgcctgcgg gacacggagc gcaatcgcaa
     1021 gcccccagta gaccaccccc gccgccgcca tcggcacaaa cacctcgccc agtttttgcg
     1081 ggagtgtggc atgtccccac cgcatccccc acaggtgctg tccgctccag gccacccccc
     1141 ccgtcaccgc cgcgatcgcc agcagcctca gggcgacgga tcgcaggggg atgaaatcga
     1201 gtttcggcat cttcttgcgc agcgcccgca tcaggagcca gacattgaca tatgcggtga
     1261 tgctgttcgc catgcccaac ccgagctggc ggaacggacc gatcagccac agcgagcaga
     1321 ccagattcag caccaggcag gccacgctga tcttcatcgg cgtctgcgtg tcccccagcg
     1381 cgtagaacgc ccgcgccagg acgttgacgc tcgaaaaagc cacaaggccc ggggccagac
     1441 ccgtcagggc catggcggcc cggtgcgtcg aggacgcggt gaattggccg cgctcgaaga
     1501 gcagacgcac catcggttcc gccagcgcaa gcaggagcgc cgcggccagc aggttgacga
     1561 agagaaggta ccccaggccc tcccgcaggg tggcgcggaa ctcggggtac ttcttctccg
     1621 ccgcaaggcc cgaaagcgtc ggcaacaagt acgtggcgaa tgaaatcccc accacccctt
     1681 gcggaaactc catcagccgc accgcatagt cgaaggaagc caccagcgtg cggtcgacat
     1741 agaacgcaat gaacgcgatg atcaggacat tcaactgaaa tgccgcaact cccaccgtcg
     1801 ccggcagcat ccggcgcaca acctcacgca ccgtcgggtt gttccaaggc cggatccagc
     1861 ggtaccgaaa gccgtccttg cgcagcgtcg gccattgaaa acccagttgc gccacccccg
     1921 cggccacaac cccgagcgcc agcgcaaaaa cctgggtctc caactcatcg ccgaatcgcg
     1981 gcgccagcag caacaccgcg gcgatcagga ccacgttcag cattgtcgcg ctcatcgccg
     2041 gaacaaagaa gtagccgcgc gcgttcaaca accccatgca gagcgccgcc acgcacacca
     2101 acagaagata cggcagcatc acctgcaaca gcctcagcac cagccgcgtg tcttccccaa
     2161 gaatcggtat cggaaacgga ggaaacaccg catcgccgcc cgatgagaaa cgcagcgagc
     2221 cggcggcgag caccaggctc acaatcgcca gacagactcc aaccacgatc gaggcaaaca
     2281 ggatcagtcc cgacaacacc gcgttcgcgg attcccacat cgccccttcc ccttccgtcc
     2341 tctccttttg cttgaagatc ggaatgaatg ccgccgtcag cgccccttcg cccagcagcc
     2401 gtcggaacag gttgggggcc tgatacgcca gcttgaatgc gctggccacc accccgtctc
     2461 ccatgaaccc cgcatacacc tgctcccgca ccaggcccag cacacggctg atcagcgtgg
     2521 caacccccat cgcccccgac gacttgagca tttgcgacat gcgtgtccgt gcgctttgcg
     2581 gccgacgccc ctttgcctcc gctctcagcc gccctccatc cccacagccc gaaccgccag
     2641 acggtctccg tcctcggcga tcctgaatgg gattctacca agcgcgcccc ggatgcgctt
     2701 tcgcgtggcc tgcatgtcct gccgcaaggc ccgcgccaga tgcacgaaca gcaccctccc
     2761 gaccggcttg tccttcagat atgcagccag aggctcgacg tccaagtgag ccagctcgca
     2821 cacgagaaga ttcaccggct tgctcaacaa aggcgacaaa tcctcgaccg cccccacatc
     2881 cgccgagtgc gccacccgca acccgtgccc ctcgatcagg aaaccgaagc tctcaaacct
     2941 ttgagcgtac ttcgatccga aactcgcccg cagttggtcc aaatgcgtgg tcgggcacgg
     3001 cgtcaccttc actcccccaa ccaccaccga acgccgcccc ctcagagcct taaaccgcag
     3061 gcggaaggcc agcaagtcct cgaacacgca gcctgcatcc ataagccgct tcacaggaga
     3121 aatcccgtcc tcgggcatgt gaactgtcag agcccgccgc cgcccctcga gccaaaagct
     3181 ctgcatcagc atgaagaaac ctcccaaatg atcgaaatgc agatgcgaca aaaaggcaca
     3241 ttcgatcagg tcgtagctcc atccactcgc cttcaccgtc cggctaaccg attcgccgca
     3301 gtcgatcaag aacgccgtct gaggaaaccg gtacagaaaa gccgagtgag agcgatcggc
     3361 acagggccaa ccgtcacccg tgcccaggca gttcagaatc agggaatcca tgagctggcg
     3421 ggatgttcgc aaatcggccg atgatcgcaa cagcaaacag gacgcgtgca agcaagacgc
     3481 gaaacagaca ggctgggcga caggggggcc gtttcccctc gggaacctca ggcggtcatg
     3541 gagtcagttg aagcgcatgc ttggtcggca gagggtcggc agggatcggt tcgtggtgag
     3601 tgcctgactg agcaatgttg attatcaatg gcttgcgatg ggagcgcgcc gggagaggtt
     3661 tttctgttca gtctggcact ggtagtgcta tcaattgata tgaaaattcg ttgacactcg
     3721 gaatcgattg tctagagtgc gcgaaccgta acccgaaacg agtaaaacaa aaccaaagac
     3781 cgtatgagcc gactacttcg taaaaacaaa aacggggctt tcacgctgat tgaactattg
     3841 gtcgtgattg ccatcatcgc catccttgcc ggcatgttgc tgcccgcgct tgccaaggcc
     3901 aaggccaagg cgcaaaggat taactgcgca aacaacctca agcaggttgg gattgcattc
     3961 cgtctgttcg ccacggacaa cggggatcgg ttcccgatgt ctgtgaccac gaacgaaggc
     4021 ggcgtgtccg actggttggg caccagcccg aacatgctgg ccgtgaacac gtatcgcgtg
     4081 ttcgccgcgc tctcgaacga gttggccacc cccaagaccg tggtctgccc gtctgacagc
     4141 ggacgcgttg cccccagcaa cttcgtcggt atggtcaacc tggccgactt cgccaagggc
     4201 gccaagaacg gcggcgtcag ctacttcgtc aaccctgatg cagacgagag ccgtccgcag
     4261 gtgattctcg ccggtgatcg gaatatcacc aacaccaccc tcaacccgag cgttggcggc
     4321 ggcacggcct acaacaagca gtcccccggc ctcaagtgga acctcatgga caccaccatg
     4381 aagaccaccg gctggtccac cgcgatgcac aacggtgccg gcaacatcgg tttgtctgac
     4441 ggcagcgtgc agcaagtcac cggagcccgc ctgcgcgagc agatcaagaa ctcccaggaa
     4501 gatcacttgc tgctcttccc atacgttcct aacaagaact aaaccgttct cgatcttttc
     4561 aattcgaagg caccctttcg ggtgccttct ttttttctaa cccgcatggc ggtcttctgc
     4621 gacgtctgac cgccctgcgc actgacgggg tgcaaagatg cgggttagcc ctgcagtcag
     4681 ccgaatgatc tccaccagcg gtgtcgatgg acgaaaaccc gtcaagcgct ccaacttgtc
     4741 gacggatggt cgacggcgcc gcatatcctc gaatccgggc gcgtacgcga catcataggg
     4801 aacgaactcc agttcggatc tcgacttgag aagttctttc accaggagcg ccaggtcgcg
     4861 gattgtggtt tcttcggtac tgccgacgtt gaaaacgccc ccccaggccc ccgggcagcc
     4921 gcccagccgt atcaacgcct ccaccgtgtc atcgacatac gcgaagcacc gtgtttgcag
     4981 gccgtccccg aaaagccgca acggctggcc ggctcgggcg gcggcgatca agcgaggcaa
     5041 gaccatgccg tatcgccccg tctgccgcgg gccaacggtg ttgaacacgc gcgcaatgat
     5101 gaccggcaac ccccgctcac gcatgtacgc cagggcgagg aactcgtcca tgagcttcga
     5161 gcacgcgtag ctccagcgcc cgagattcgg agggccgatc agcagatcgt cctcttccct
     5221 gaattcctcg ttctggctct ttccataaac ctccgaggtc gaggtcagaa gcaacggcac
     5281 cccgtgccgt ccggccgccg ccaggatcgc ctccgtctcc tggatgttcg tttcgatggt
     5341 ccgaatcggc gagcgcacca cgagatccac accgacggcc gcggcgaggt gatagatcag
     5401 cgaggcctcg cctgccaagt cagccagact cgcgcactgc gagacccgcg cctcgattac
     5461 acgtagacgg gggtgccctt ccacccgacc cagattcgca cggctccccg tcgagaaatc
     5521 gtcaacgatg gtcacgctcc gcccctccgc gagaaggcgc tcagccaggt gcgagccgat
     5581 gaacccggcg cctcccgtga tgaggacgcg atcgcccatg aacgcgcttt gacctactgc
     5641 caaatcatct ccttgacggt cttggccgcg ggaatgaacg gcatcacgtg ctccgtatag
     5701 ggcgtgataa cgtcaagcac atagggctgg tccgagtcga gcatcctctg catcgccgcc
     5761 cgcaggtcgc gcttgtaaat caccctttcg cacggaacgc tgaaagcctt cgcgatggcc
     5821 acgaaatccg gatagatttc ccgcatgttg tcagggtcgc ccaggtaggt gttcccgcga
     5881 ttgccctgat aaaaccggtc ttcccactgc atcaccatgc ccaggtgctg gttgttcaaa
     5941 atgagcgcct ttgccgcaat cccctcgatg tgcgccgtcg ccaactcctg cacgttcatc
     6001 aggaatgatc cgtcgccgtc gatgtccaca acctgccggt ccggaaacgc cactttggcg
     6061 cccagcgccg ccggataccc gtagcccatc gaacccaaac cggcgctcgt caggaattgg
     6121 cggggatgcc tgaactgata gaattggccg gcccacatct ggtgctgccc caccccggtc
     6181 gtgatgatcg cctcgccccg ggtcaactcg caaagcagag caacggcctc ctgcggaaga
     6241 atcacctggc tgaagtcgcc acccatgtga tcgcgcacgt gttgcgattt gagcacctcc
     6301 tcggtgatat gatagcagag cggtgctttg gacttccatt ccgcgatctg gcggtgccag
     6361 ggctcgtacc tcttctgcaa ggggcgtttc gccagccggg cattgagctc gctcaaggca
     6421 aacctgatgt cgctgatcac aggcaggtgc accttccggt tcttgttgat ctccgacgcg
     6481 tcgatgtcga tgtgcacgat cgttccgtgc tcgcagaact tgtccacctt gcccgtcacg
     6541 cggtcgtcaa accgcacccc gaacgcaagc agcaaatcgg cctcggcaac cgcccagttc
     6601 gcgtacgccg tcccgtgcat ccccagccac ttcagcgaga gcggatcgtc ctccggaaaa
     6661 gccccgcacc ccatcagggt cgtcgtcacc ggaatgcgcg tcgccgccac aaaacgacgc
     6721 agttcgtccg aagcgccgct ggtgatgatg ccgccgcccg catacacaac cggacgctcc
     6781 gccttctcga tcaacgcgac gatctcgtcc agcgccgctt cgtccgcccg ccgattcgga
     6841 ttgtagccgc gcagttcgat cgaggacggg aagaccggct gcgcgcgctg ctgctgaatg
     6901 tccttgggca ggtcgatcaa caccggcccc ggacgcccgc tccgggcgat gtagaacgcc
     6961 tccttcacaa cgcgcgggat ttccccgata ctggtgatca gatagctgtg cttcaccacc
     7021 ggaagtgtca tcccgtagat gtccgtctcc tgaaaagccc ccttgccaat catcgaacgc
     7081 accacttggc cggtgatcgc cacgatcggc accgagtcca tgtacgcgtc ggctatcccc
     7141 gtcaccagat tcgtggcgcc aggaccgctg gtggccatgc aaacccccac ccgacccgtc
     7201 gcgcgcgcat atccctcggc cgcaaaggct cccccctgct cgtgccgcgg cagatagacc
     7261 cggatgttct tggacttgca cagggcttga tgaatctcca tgctggcgcc gccaggatag
     7321 gcgaaaatcg cctccacacc ctcccgttca aggcatttca ccagaatctg gctcccgagc
     7381 agcggagcgg aagactcggg cgcggccttg ggagtgcttc ccgcgccgtc acgcccgatg
     7441 gggcgaaccg gagtccgggt ccgcgccaag gaggcggcgg tctcgggcgc ggtcttggaa
     7501 gtgtcgccag atggaggcgt gttgcgggct gttgtcttgg ggtgtctgtt catgtatttc
     7561 tacgcttgta gcgaccgggg tccgggtaac aaaaaaccca cgaccgtcgg ccagccgtgg
     7621 gtggtttgtc gaaatcctct taagcacgac aaaaccccgc ggcatcgccg cttacgacga
     7681 cgaccagcaa caggttttgt cctgcattcc gtaacatacg ccgacacatt agggaaatcc
     7741 cccaacccag tcaagcgacg attatggacc gtttttcagt ctttcagtcg ttcgatcctc
     7801 gggaaataga gccagagatg gcccagatcg tcgtcctcgc cgaaaacgat ctggccgtcg
     7861 cgtccaacca cagcatcgcc aaacaaatag ccgtaacgcc gcctctcgag caccgacacc
     7921 gccaccccta aatccgacac cgcccgcgcc gcgggattgt aacagaacat cctcgccatc
     7981 tcgctcccgc aaaaaccgaa cacccggccg tcgtgcgtga ccgccatcgg accgaccggc
     8041 gccagcgcgg tcaggcccaa aacaggactg aatcctcgat cttcgtccag cgcaaacagc
     8101 ctgccctgac cgtccgccgt gtagagcagc ccgctccgat tgtcacgggc ccaggtcatc
     8161 ggcgacttgt cccagacacc ctcgggcaac ttcaacgcgt ggcgcgtgat ggtccgcgac
     8221 gccgtgtcgt aacgccagag atcacccggt ccgtcttgtc cgaacaccct gcctctcgac
     8281 ccaacggcaa tgcgtccggc accggcgacc ggacccacga tcgcaatcgt ccccgtgtca
     8341 acgtccaccg tgaaaagcgc gcctgtggtg atgcccacaa tcgaaccgcc cccatcggcc
     8401 acggcgtgga cgatccgttc gccgttcacc ggaccgagat cccgcagcgg cggacgcccg
     8461 aatccccact cctgaatcag atcgaacggc aatccctgca actctccggt gatcacccgc
     8521 ccgccgcccg cgccgttgac acaggcggca aaccgtgtct tgccgcagca gaccgccgcg
     8581 caatggtcgg ccccctcgat tgccccccga tcaaaaacaa tccccgtcac gccatgaaag
     8641 atgccgacaa acagatgcgc cgcacgcccg ctggtcccgc cgtagatcat tccatctccg
     8701 gacacggcca gggcggtgat atggctttcg tccgccggta tcggagtgct ggcgccaggg
     8761 aaacaggtcg gaaaggccgc cattgtccct tccttcatga acgttccgtg gacaatctcg
     8821 tcgacaacgg ctcgcgcctc gggcgagttc atgtcgtatc gcttcctcgg tttctcgctc
     8881 ataagattct cacccccaaa tccggatcgg caaatgcgcg ttttccttcc tgcgcgacgc
     8941 gggcaggtgc acctggaaga tgcccaccgg tttcgtgccg acgacgccga agaacagatt
     9001 cccgttatga tcgaccgcgc cgcgcgacac gtactgggcc gggtgggtcg gatgctcgag
     9061 aatggcgacc tcggtgcgct caagcgtctt cgggttcagc cgccagacca caccctcccg
     9121 cggatgcccg ctctcgagct tgggctggta gacaggatcg cgccagcgcg atgccacata
     9181 gtacaactgg ccatccccag cgaagaccaa tccgccgcag tgatcgatga atgtgtccac
     9241 cggaatgctc gaatcgcggt cctgcgtcgc cggacccaaa tcctcgactc gcggccacgg
     9301 accttcgttg ggccagaccc gcatcaggcg cggatgggcg atccacgtca ccgcgaagac
     9361 gctctccccg tccggtgccg ccgccacgtc gtagaacacg ctgtgccagc cggtctggaa
     9421 ttgcgggttg tgcggcagca cgaagggcga acgctcgagc cggtccgtct ccggatcgta
     9481 gcgcaccaga tgcccgtagt cgtcggtggt ccagacgcga tgtttccggt cgagaaagag
     9541 ggcctgggcg ttgatcgacc cgatgcgacc cgcgtcccgc cgcgcccgtg tacgcgtgtc
     9601 gtaaatgatg aagtgatccc gcggatagct caacgcatag agcagatggc gctcctcgtc
     9661 atgcagcagg cagcggcaac cctccttcgg gatcatcgtg tcccaccaca acacctcgtc
     9721 cttgcgcgtg tcgaatgcca gcagcgcgct ccctcgaaaa cagcgctcgg gatcgtgcca
     9781 cgaacgccac ggcgagtaag ccggctggtc aatgggcggc cccgacagat gcgttgccat
     9841 gtacaacacc ccgtccgcca tcgagggcgc gaagctgtag tgaatcttgc actgcgtcgc
     9901 tcgcccggaa tcgctcgggt cctcgaccat ctcgtccaac gcaaacagat aatcgagcgt
     9961 gtccttctcc tcgttgtagc gcgccacttt cacgacgccg cccggaacac tctcggcgca
    10021 tgccgccgca taaatccgac cgtccgggcc agtcgacaac gaccagcacg tgtcggcatc
    10081 cggatggccc gtgaacggat accacgagtt gcggtagggc cgcggctcgc cgtccgcgcc
    10141 ggtgtgttcg cccgtcccga gtttccccgc catcccgggc agagccagcc aagtccccgc
    10201 ggccaacgcg ccgcctttca aaaagtccct tcgcttcagt ttgcctgcca taacgcgcct
    10261 ccagaggatg tccgcccgac cctgccacca acctcgtccg ctgccaaggg caatgcgcgt
    10321 tcaaccgcgg tcgccggtgc atctcagaaa tcggcgagca actggcctcg cggagggaac
    10381 tggtgacaca ctgcgcaaga tgcgtcggcg ccaaagggct caaaccatag gattgttatc
    10441 tgggcgggag tgtcgcccgg ttcgtgatgc gcgattgtca actcagacgc gatcctctgg
    10501 ttcgaggcgc gcggacgggg tcgagcccga tcgaccactc gaaaaacagg gtcccccccg
    10561 gagttccgaa gccttacata gaggttctca ccgtgtcgct gtttgagagg ctaggcagca
    10621 aacctattcc ttcgaggcgc cgccattgtg cttgggagcg atagacgcca gtccggtcat
    10681 cgtgagcagc ggcagaacga cgaacctctc atcgtcatca taaacggtga caaacgtccc
    10741 cttgggggaa atcgatatgt aatccctgtg tggcaccgca tactcgcggc catccgccat
    10801 ccggagcgtg aacgccgcac cgctctctat ggctgactcg atctggcttc gcgtcatgaa
    10861 tcactgctac gctgccaggc atgtgtacgc aagcgctttc ttccggccaa cgcccgggtg
    10921 agacacggct accgagccac cacggcagtc accaagcccg gccatagaag cgagtgatgg
    10981 ggtctccggg tccgtcgagt atctcatggg atacactccc tccctcgggc ttcgcatctg
    11041 gcccgatggc actcccgcca aaaccggaat caatttttgc acagacccca agtcaagctc
    11101 gccgaatttt ctcctttcct gtccgctccc aggcgacacc atgcggtcgc tccggcacgc
    11161 aagcacggcg ggacgaggcc gccgttcttt gcccaggagc caggagagcg tatggatttg
    11221 aaaagaacga aagcccttgt gacgctgctg atcgcgcttg gcgctgccaa tgccggcccc
    11281 ggcgtcaacg ccaccgcaag ttcgagcacg cccctggcca aaccgaatat catcgtgatc
    11341 atggccgatg acctcggcta cggcgatgtg tcatgctatg gcgccaccgc cgtccagacg
    11401 cccgccatcg accgcctggc acgcgaaggg gtgcgcttca ccagcggcta ctgctccgcc
    11461 tcaacctgca ccccttcgcg tgccaggcgg tcgatggcgg gcgtctggac ggc
//
LOCUS       DAOQRO010000070        11295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4935_length_11295_cov_5.536833, whole genome shotgun sequence.
ACCESSION   DAOQRO010000070 DAOQRO010000000
VERSION     DAOQRO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_11295_cov_5.536833"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PKK20_04110"
     CDS             complement(<1..1266)
                     /locus_tag="PKK20_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99102.1"
                     /translation="MSNTPEFELDLDLQLLPAWARQPSTENRYAKYEGDTEGDRRDWR
                     GDRFGRRDAGRNRPPRRDRLPGGPGRGGPAGRGEGRGERGERGRGPSPEGRPAAPRWQ
                     REEPVVALPDVEIGFTPEEAGVESLARQIKLTGRAYPLFGIAELILKRPDRVHARFHA
                     KKGTDGAVAQPLVVCSLDDSLWLSEDEAVAHILRHHFATFYQIEKVPTDPPKGTYTFV
                     AQCGMSGTIFGPPNFHDYQDKLRKFHAERYARMPFDVFKARVKIVKDEAVVKKWIEEQ
                     SAKLEYICLNVPESCRFGSREDVEKHFRATHLPNLVRSVDSYTLRAGAKRPPLSPGLQ
                     TLLRHAIEDQKRFPLKVATVLSQQFAGHGLQFFKVNRTVTHVCVARPHYLDLENTVVS
                     DGVRKIVDFINTHPASTRRKLIQALAPPPP"
     gene            complement(1411..1674)
                     /locus_tag="PKK20_04115"
     CDS             complement(1411..1674)
                     /locus_tag="PKK20_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99103.1"
                     /translation="MKTAASNQGSERGSAVIVVLALLFLTLAFIAGNDVTLRSLRREM
                     QIIEERQLRKYEPPEPGSTQTGPSWKSSDSRPWRDHVASGSRP"
     gene            complement(1671..2240)
                     /locus_tag="PKK20_04120"
     CDS             complement(1671..2240)
                     /locus_tag="PKK20_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99104.1"
                     /translation="MNTRRVAMRMGPRRRHAWSERAAGGQPCSPQSGILLIECLIYLA
                     CLFAVLGAALAVFYQTLDFSRDLRRNADDIARVLKAGELWRTDVRSATGRIVFEEGPG
                     GRTLRIPREGGATTYFFVTNAVLRCAAATGRCETVLPAVKECRFVSEPRQRVTAWRWD
                     LELPSRLEAVRVHPLFTFAAVAGPEEAQP"
     gene            complement(2237..2623)
                     /locus_tag="PKK20_04125"
     CDS             complement(2237..2623)
                     /locus_tag="PKK20_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99105.1"
                     /translation="MVTRSSGRRRALMRRGALMTELVVAISIVVVALFPLALAFLNEQ
                     KLCRAYYYRALALEIVDGEMEVLAAGEWRAFESGVHAYQPLAPARTNLPPGRFELTVS
                     DRAIRLAWQPGRRDAGGGVVREVKVR"
     gene            complement(2617..3753)
                     /locus_tag="PKK20_04130"
     CDS             complement(2617..3753)
                     /locus_tag="PKK20_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU99106.1"
                     /translation="MNPLVLNLLVWLLELMALAVLAYLAYFFLSLPLRRRERARLFLD
                     LIELGLRDGRSVEQTVCALGATGDRALGSRFHRFATHLASGLTLAQALERVPGLLPPP
                     VAGMLRAGHEGGDLPGVLAVCRRRLQDHVDAVAAAHHYFMLFALVVSPAWIATFAALF
                     VIVMPKFLAIAADMGTTPALLIALTSHSKILVVAQTALLLGLYGVILAYVGGPRLAGW
                     ISRIWPGLPDRVAWLFPWKRLRLQRDFSSVLAMLLDAGLPEARALDLAGESTANAVMR
                     GRAVGAVTDLERGAGLPSALRRLDASGQFRWRLENAMQGPARFRAALDGWHEVLSARA
                     WQLEQTAAQLATTGLVLVNGLFVGVLALGVFGMLLSMIEGGFLW"
     gene            complement(3761..4762)
                     /locus_tag="PKK20_04135"
     CDS             complement(3761..4762)
                     /locus_tag="PKK20_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU99107.1"
                     /translation="MKLDEFAFLNQQLAGMLRGGIPLEGALKQLTATLERGVLREELE
                     QLQADLAKGTPLREALPRRRLPELYQQLVQLGAQGNNLPGMLTLLADYYQRRHLIWTK
                     LKALMVYPAIVLIGSLGLSILLAHLARVLTTSVPWFFYEGQTLPTAAAIGGPSALLIP
                     PLWIALMLVLFVAALTVPACRNWVRWHLPGFRESSLSQFAATMRLLLAAGTDLPRALA
                     LVANLEQGTAVGRELAGWRDRLAAGAGRISEFATPSRLFPPLFVWLVGQGGENLAGGF
                     ARAAELYGERARHRVEMMLYAALPVAVLALGGLIAGQLVLGLRVCALVTGLNMLGTE"
     gene            complement(4759..5814)
                     /locus_tag="PKK20_04140"
     CDS             complement(4759..5814)
                     /locus_tag="PKK20_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU99108.1"
                     /translation="MQAPEVLSRLLQQAEAAGASDIHLHLTGGQTEVAFRLDGLLTPI
                     TTLETGLGEHVIGRIKFLARLKTYQDSLPQDGRIDRRDSGAGCDIRVATYPTVRGEKV
                     VLRLFNRDGVRALDELALPDELVSELEAFLRQETGLLLLTGPAGSGKTTTIYACLRAL
                     ARGGRKHVITVEDPVEQILPGVMQTEVNEARGLTFAVAARHLLRQDPQVLVLGEIRDD
                     ETATLAVRASLTGHLVISTLHAGSCRGVFERLLAMCPDHAAVASAVTLVVNQRLMRRC
                     CASCQRRGCPLCLQTGYRGRLPLVEWVRLTPALQRQLRTVGPDAIEPARSLELAARDL
                     VAAGLSDQQELERMFGL"
     gene            6064..7170
                     /locus_tag="PKK20_04145"
     CDS             6064..7170
                     /locus_tag="PKK20_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU99109.1"
                     /translation="MAIRVLIIEEQSINRAGIRSHLETDPGIEVLGEVGDAAAGLSAV
                     NASQPDVVILGASFCRTRGEPVLGRLLRVSQPPAVLVLSEREDAWIAERVLRHGAMGY
                     LSNDVNANTLASAVRQAARQEIVLDPRLGNRLLRHLASGFPTSEQDPPQVLSSREMEV
                     FRLTGQGKEAKEIAAELSISSRTVDVHRANIRTKLRIQGTHELMRSAMEWEHHHRHER
                     RLRAFCQERTPLLLVEDDEVDILSVQRALRELETETRLVVARSADEALRFLRSPENPR
                     PGLVLLDIKMPGMNGEEFLAEIRRDPDLRNLPVVVLTASRLEEDKSRMYALGISGYLM
                     KRANSAEFLEMVGLLAQYWSLNEPPPSPTPKAKS"
     gene            7171..9042
                     /locus_tag="PKK20_04150"
     CDS             7171..9042
                     /locus_tag="PKK20_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99110.1"
                     /translation="MDPLPSKRDLKALPHDQGELIRLLILDPWFRIVSVLILVVVVGI
                     AISLPKIWRVTPPGFQPQVRVSLLNLAQAWQLRRSARHLAANGSDEEAAAAWRAAVIK
                     NLGHLPTIRQALRSTVSLPNPPAAALEQAAKLSAWLVALGGTNAQDIGLVAQLCDRTG
                     RSDEVYDLLHPLRDELSREDEVLYLKALLDRGDGPAYRARWERLSAESAQDPALALYH
                     AAYLAGWGPLPARDDARRRLEQALENPAHRILATRLQLLDFEQAQDPHNYAKSLERLV
                     ESRADRLRDHLGYWKLLAQAGRSDEARTLAAREVGKPLSAKEAILLADALNALGLPDQ
                     AHLTLRHFTTQQGGNPSAATQSVWLKRAALLSAAADWRGLLEMAEELRAVPQTARALG
                     GWTDFIEGRAHHMLKHHDQADASMQAAVRGFFPSDRVALEVGLGLKELGYYDKAWQVL
                     APLEQPLKDVPHYWQVVFELAEAMRQDPVLLLRAATEARRLLPDDDRWDFNYAAALLI
                     NRLDPARVVSVTRDFLVRMPASPHARVNHCYALALNQRWNEAAELLSTVDPARFDEFG
                     RSVFHLCAFEIHLAQGQTNLARLDAAEIRREHLFPNQIRWLDAALRDTGFADLIPPR"
     gene            complement(9088..10452)
                     /gene="dnaA"
                     /locus_tag="PKK20_04155"
     CDS             complement(9088..10452)
                     /gene="dnaA"
                     /locus_tag="PKK20_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNU99111.1"
                     /translation="MQTSIEKVWSSAQSILRGMLNADIYNLWFTHLRAREMSEEGIVV
                     EVPNDFCELWLKDNYLGLLQDVVAHVTGSTRRVQFVIAAKPAEPTPNEEAAPQRVAVS
                     AAHGPIAGAVGRESPFNPNNTFDTFVVGSNNSFAHAAALAVAQAPGRSYNPLFVYGGT
                     GLGKTHLLHAIGQHAAQNRKGARVAYLSSEKFTNEYIDAIQTNQLVRFRKKYRMTDVL
                     LIDDVQFLANKERIQEEFFHTFNALHEAHRQIVLTCDRPASEIQNLEHRLMSRFEWGL
                     VTDLQPPDVETRVAILRKKEQSLGVSLPQEVIDFLAQRIRSNIRRLEGALIRVATYAS
                     LAGKPVTLEVVEHQLRELLHEEGRHAITIEIIQKRVAEHYDIRLADMTSRRRPENIAF
                     PRQVAMYLARQLTESSLNTIGEAFGGRDHGTVLHACRLVRGRMEVDPSVRQVVSYLEK
                     QLHR"
     gene            complement(10981..>11295)
                     /locus_tag="PKK20_04160"
     CDS             complement(10981..>11295)
                     /locus_tag="PKK20_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014950115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNU99112.1"
                     /translation="KPFKLEDVKEALAQMGIEGMTVSEVKGFGRQKGHTEIYRGSEYT
                     VDFLPKIKIELVIAAQLVEQAVEAILKAAKTGKIGDGKIFVSSVENAIRIRTGEKDEE
                     AV"
BASE COUNT         2095 a         3787 c         3543 g         1870 t
ORIGIN      
        1 gggggggggg ggggcgagcg cctggatcag cttgcgccgg gtggatgcgg gatgggtgtt
       61 gatgaagtcg acgatcttgc ggaccccgtc ggagacgacg gtattttcga ggtcgaggta
      121 gtgaggccgg gcgacgcaga catgagtcac ggtgcgattg accttgaaga actggagtcc
      181 gtggccggcg aactgctggc tcaggacggt ggccaccttg agggggaatc gcttctgatc
      241 ctcgatcgcg tggcgcagca gcgtctgcaa gcccggcgag agcgggggcc gcttcgcgcc
      301 cgcgcgcagg gtgtacgagt cgacggatcg gacaaggttc gggagatgcg tggcgcggaa
      361 gtgcttctcg acatcctcgc gactgccgaa tcggcacgat tcgggcacgt tgaggcaaat
      421 gtactcgagt tttgcgctct gctcctcgat ccatttcttc accacggcct cgtccttgac
      481 gatcttgacc cgcgccttga agacatcgaa cggcatgcgc gcgtagcgct cggcgtggaa
      541 cttgcgcagt ttgtcctggt aatcgtgaaa attgggcggg ccgaagatgg tgccgctcat
      601 gccgcactgg gccacgaacg tgtaggtgcc tttcggggga tcggtgggca ctttctcgat
      661 ttggtagaac gtggcgaaat ggtggcgcaa gatgtgcgcg acggcctcgt cctcgctcag
      721 ccagagcgag tcatccagcg aacagaccac cagcggctgc gccacggcgc catccgtccc
      781 cttcttcgcg tggaagcggg cgtgcaccct gtcgggacgc ttcaggatca gctcggcaat
      841 cccgaacagc gggtaggcgc ggccggtgag cttgatctgg cgcgcgagcg attcgacacc
      901 cgcttcctcg ggcgtgaagc cgatctccac atcaggcaga gcaaccaccg gctcttcccg
      961 ctgccaccgc ggcgctgcgg gccgcccttc aggactcggc ccgcgcccgc gttcaccgcg
     1021 ctcgccccgc ccttcgcccc gccccgccgg accgccccgg ccggggccgc cgggcagtcg
     1081 gtcccggcgt ggcggccggt tgcgacctgc gtcgcgccgg ccaaaccggt cccctcgcca
     1141 gtcgcggcga tccccttcgg tatcgccttc gtacttggca taacgattct ccgtggacgg
     1201 ctgacgcgcc cacgccggta gcagttggag atcgagatcg agctcgaact ctggagtgtt
     1261 gctcatcaaa tcttcgtccg ggcctttgaa ggcccggcac cgtttagtgc ttcaaacaac
     1321 ggcagcatgg ggtcgagccg aggggaatgc aagcctcaga cagacgcaac actcgacgct
     1381 cagtgttcga ctgaatccgt gggaaaggga tcagggacga gaaccagacg cgacgtgatc
     1441 gcgccatgga cgggagtccg aggatttcca gctggggcct gtttgggtcg acccgggctc
     1501 aggcggctcg tacttgcgaa gctgacgctc ttcaatgatc tgcatctcgc ggcgcagaga
     1561 gcgcaaggtc acatcgtttc cggctatgaa cgccagggtc aagaagagca aggcgagcac
     1621 gacaatcacg gccgagccgc gctcgctgcc ttggttggaa gccgctgtct tcatggttgc
     1681 gcctcctccg ggccggcgac cgcggcaaat gtgaaaagcg gatgcacccg cacggcctcg
     1741 agacgcgacg gcaactcgag gtcccagcgc cacgccgtga cgcgctggcg cggttcgctc
     1801 acgaaacggc actccttgac agccggcagg accgtctcgc atcgcccggt tgccgcggcg
     1861 caacggagca ccgcattggt gacaaagaaa taggtagtcg cgccgccctc ccgtggaatg
     1921 cgcagcgtgc gtccgcccgg gccctcctcg aagacgatcc ggccggtcgc ggaccgcaca
     1981 tcggttcgcc agagctcccc ggccttgaga actcgagcga tatcgtcggc gttgcgccgc
     2041 aggtcgcggg agaaatcgag cgtttggtaa aacaccgcca aggccgcgcc aagcacggca
     2101 aagaggcacg caaggtagat caggcactcg atcagcagga tgccggattg cggggaacac
     2161 ggctggcccc cagcggccct ctcggaccag gcgtggcgcc gcctcggccc catgcgcatg
     2221 gcaacgcgtc tcgtgttcat cgcaccttca cctccctcac tacccctccg ccggcgtcgc
     2281 gccggcccgg ctgccaggcc agccggattg cccggtctga gaccgtcaac tcgaaacgcc
     2341 ccggagggag gttcgtgcga gccggcgcca agggctgata agcgtgaacc ccggactcga
     2401 acgcgcgcca ctcccccgcg gccagaacct ccatctcgcc atcgacgatt tcgagggcca
     2461 gggcgcggta gtaataggcg cgacacagct tctgctcgtt gaggaatgcc agagcgagcg
     2521 ggaagagggc aacgacgacg atgctgatgg cgacgaccaa ctcggtcatc aacgcgcccc
     2581 ggcgcatcag cgccctcctg cggccggaag aacgggtcac cataaaaagc ctccttcgat
     2641 catgctcaga agcattccaa aaaccccgag tgcaagcacg ccaacaaaga gtccgttgac
     2701 cagcaccagg cccgtggtcg ccagttgggc cgccgtctgt tcgagctgcc acgccctggc
     2761 ggacaggacc tcatgccagc catcgagggc cgcgcgaaaa cgggccggcc cctgcatcgc
     2821 attctcgagg cgccatcgga actgacccga cgcgtccaat cgacgcaacg ccgagggcag
     2881 accagcgccc cgctcgaggt ccgtcacggc gccgactgcc cgaccgcgca tcaccgcatt
     2941 ggcggtgctc tcacccgcca gatcgagtgc gcgagcttcc ggcaggccgg catccaggag
     3001 catggcgagc acagacgaga agtcgcgctg gagacgcagc cgcttccacg ggaaaagcca
     3061 ggccactcga tccggcaggc ctggccagat gcggctgatc catcccgcga gacgcgggcc
     3121 accgacgtaa gccagaatca ccccatagag accgagcaac agcgcggtct gcgccacaac
     3181 gagaatcttg ctgtggctgg tcagggcgat caggagggcg ggcgtcgtcc ccatgtccgc
     3241 ggcaatcgcg aggaacttcg gcatcacgat cacgaaaagc gcagcgaagg ttgcgatcca
     3301 cgcggggctg acaaccaggg caaagagcat gaagtaatgg tgggcggcag caacggcatc
     3361 gacgtggtct tgaagccggc ggcggcacac ggccaggacg ccgggtaaat cgcccccctc
     3421 gtgcccggcg cggagcatcc cagccaccgg cggggggagc aggcccggca cccgttcgag
     3481 agcctgggcg agggtgaggc ccgacgccag atgcgtggcg aagcggtgga acctcgagcc
     3541 caaggcgcga tcccccgtcg ctcccagggc gcacacggtc tgttccacgc ttcggccatc
     3601 tcgcagcccg agttcgatga gatcgaggaa gaggcgtgcc cgctcgcgcc ggcgcagggg
     3661 gaggctcagg aagaaatagg cgaggtaggc cagcaccgcc aacgccatca gctccaacag
     3721 ccagaccagc agattgagca cgagagggtt catggccgca tcactccgtg cccagcatat
     3781 tcaatcccgt caccagggcg cacacccgca gaccgaggac caactgtccg gcgatcaacc
     3841 cgcctagcgc caggaccgca accggcaacg cggcatacaa catcatttcc acccgatgcc
     3901 gggcgcgctc gccgtaaagc tccgcggcgc gggcaaagcc gccggcgagg ttctcgccgc
     3961 cctgtcccac cagccagaca aacaggggcg ggaaaagccg gctgggtgtg gcgaactcgc
     4021 tgattcgtcc ggcgcccgcg gcgagccggt cccgccagcc ggccagttcg cggccaaccg
     4081 ccgtcccctg ctcgaggttg gcgaccagcg ccagcgcccg aggcaggtcg gtgccggcgg
     4141 ccaggagcag ccgcatggtc gcggcaaact ggctgaggct cgattcccga aagccgggca
     4201 agtgccagcg gacccaattg cggcaggccg gcacggtcag ggccgccacg aacaggacca
     4261 acatcagagc gatccagagc ggaggaatga gcagggcgct gggaccgccg atggcggccg
     4321 cggttgggag cgtctgcccc tcgtagaaaa accaaggaac cgacgtcgtc agcacgcggg
     4381 ccaaatgggc caggagaatc gacaacccca gactgccgat caggacgata gccggataga
     4441 ccatgagggc cttgagcttg gtccagatca ggtggcgccg ctggtaatag tcggccagaa
     4501 gcgtcagcat acccggcagg ttgttcccct gggcgcccag ttgaaccaat tgctggtaaa
     4561 gctcaggcag gcggcgacgc ggcagggctt cgcgcaaagg agttcctttg gccagatccg
     4621 cttggagttg ctcgagttcc tcgcgcaaca ccccgcgttc gagagtcgcc gtcaactgct
     4681 tcagagcgcc ctcgagcggg atgccgccgc gcagcatccc ggccagttgc tggtttaaaa
     4741 aggcgaactc gtccagtttc ataagccgaa cattcgttcc agttcctgct ggtcagacag
     4801 tcctgcggcc acaaggtcac gcgccgccaa ctcgaggctc cgcgccggct cgattgcatc
     4861 cggcccgacg gtccgcagtt gacgctggag ggcgggggtc agacgcaccc actcgaccag
     4921 cggcagccgc ccgcgatagc cggtctgcag acagagcgga cagccccggc gctggcacga
     4981 cgcgcagcag cggcgcatca gccgctgatt gaccaccagc gtgacggccg acgccaccgc
     5041 ggcgtggtcc ggacacatcg ccagcaagcg ctcgaacacg ccccggcagg atcccgcgtg
     5101 cagggtggaa atcaccaaat gccccgtcag cgacgcccgc accgccagcg tcgccgtctc
     5161 gtcgtcgcgg atttcaccaa ggaccagcac ctgcgggtcc tggcgcaaga gatggcgcgc
     5221 cgccaccgca aaggtcagcc cgcgcgcctc gttcacttcg gtctgcatca ccccgggaag
     5281 gatctgctcg accgggtcct cgacggtaat gacgtgcttg cgaccgcctc gagccagggc
     5341 gcgcagacag gcgtagatcg tcgtggtctt gccgcttccg gccggccccg tcagcagcaa
     5401 caggcccgtc tcctgccgca agaacgcctc caactcactg accagttcgt ccggcaaggc
     5461 cagctcatcc agcgcacgga caccgtcacg gttgaacagc cgcagcacca ccttctctcc
     5521 ccgcaccgtg gggtaggtgg ccacccggat gtcgcagccg gcaccgctgt ctcgccggtc
     5581 gatgcgcccg tcctgcggca ggctgtcctg gtaggtcttg agccgggcga ggaacttgat
     5641 gcggccgatg acatgctcgc ccagcccggt ttcgagggtt gtgatcggcg tcagaaggcc
     5701 atcgagacgg aatgccacct cggtctgccc gcctgtcagg tgcagatgga tgtcgctcgc
     5761 cccggccgcc tcggcttgct ggagaagcct cgacaacact tcgggcgcct gcatggcttc
     5821 gtttatcact tcgcttcgtt ggaccgtgcg gacccaactt ccgcacgcgc aaaggcggct
     5881 ggccagtccg gacctttcgc ctcgcggcat ggccgcagcc agggcgacga gacgctcgcc
     5941 catctgcggc gccgacggac gggccgggcg ccgtgctccc cgccttggga ggaggctgcc
     6001 cgaaccgcga agaggcctgc gaagagtatt gcgaaaaatc cgcggccctg ccacactggg
     6061 gtcgtggcga tccgggttct catcattgag gagcagtcca ttaaccgcgc cggcatccga
     6121 agccacctcg agacggatcc gggcatcgag gtgctcggcg aggtggggga tgcggcagca
     6181 ggactcagcg cggtcaacgc cagtcagccc gacgtggtga ttctaggagc cagcttctgc
     6241 cgcacgcgag gagaaccggt gttggggcgt ctgctcaggg tctcacagcc gccggcggtg
     6301 ctggtcctgt ccgaacgcga ggatgcctgg atcgccgagc gggttttaag acacggcgca
     6361 atgggatacc tctcgaacga cgtcaacgcc aacaccctgg cgtcggctgt caggcaggcc
     6421 gcacgtcagg agattgtcct ggacccccgc ctggggaaca ggttgttgcg ccatctggcc
     6481 agcgggtttc caacctccga acaggaccct ccgcaggtgc tctccagccg cgagatggag
     6541 gtcttcaggc tcaccggcca gggcaaggaa gccaaagaaa tcgcggcaga actgtcgatc
     6601 agctcgcgga ccgtggacgt gcatcgggcg aatatccgga ccaagctcag aatccagggc
     6661 acgcacgagt tgatgcgctc ggcgatggaa tgggagcatc accatcggca cgaacgacgc
     6721 ctgcgggcct tctgtcagga acgcacgccg ctcctgctgg tcgaggacga cgaagtggac
     6781 atcctcagtg tccagcgggc gctgcgggaa ctggagaccg agacgcgatt ggttgtcgcc
     6841 cggtcggccg atgaagcctt gcgattcctg cggtccccgg agaatcctcg gcccgggctg
     6901 gtgcttcttg acatcaaaat gccgggcatg aacggcgagg agtttctcgc ggagatccgg
     6961 cgagatccgg acctgcgcaa tctgccggtg gtggtcctga cggcctcgcg actcgaggaa
     7021 gacaagagcc gcatgtacgc cctgggcatc tcggggtatc tgatgaagcg agccaactcg
     7081 gccgagtttc tcgagatggt cgggctcctg gcccaatact ggagtcttaa cgagcctccc
     7141 ccctcaccga cgccgaaggc gaagagttag gtggatccgc tcccctccaa acgcgatctg
     7201 aaagccctgc cccacgatca gggcgagctg atccggcttc ttatcctcga tccgtggttc
     7261 cggatagtgt cggtgctgat cctcgttgtc gttgtcggga tcgcgatctc gctgcccaag
     7321 atctggcgtg tcaccccgcc cggcttccag cctcaggtgc gagtgagcct cctcaacctg
     7381 gcccaggcct ggcaactgcg gcggtcagcc cggcacctcg ccgccaacgg atcggacgag
     7441 gaagccgccg ccgcctggcg cgccgccgtc atcaagaatc tggggcatct gccgacgatc
     7501 cggcaggccc tgcgctctac cgtgagcctg cccaacccgc ccgccgcagc cctcgaacag
     7561 gcggccaagt tgtcggcctg gttggtcgcc cttggcggga ccaatgcgca ggacatcggc
     7621 ctggtggcgc agttgtgtga tcggacaggg cgttcggacg aggtgtacga tctgctgcat
     7681 ccgctgcggg acgagctctc gagggaggac gaggtgcttt atctcaaggc cctcctggac
     7741 cgaggcgatg gacccgccta cagggcccgt tgggagcggc tcagcgccga gtccgcccag
     7801 gatcccgccc ttgccctcta tcatgccgcc tatctcgccg gctggggacc gttgcccgct
     7861 cgcgacgacg cccgccgccg tctcgagcag gcgctcgaga atcccgccca ccgcatcctg
     7921 gcaacccgcc tgcagcttct cgatttcgag caggcgcaag atccgcacaa ttacgcgaaa
     7981 tcactcgaac gccttgtcga gtcccgagcg gacaggctgc gcgatcacct tggttactgg
     8041 aagttgttgg cccaggccgg gcggtccgat gaggcccgca ctctggcggc gcgcgaggtc
     8101 ggcaaaccgc tatccgccaa agaagcgatc ctgctggcgg atgccctgaa cgccctggga
     8161 ttgcccgatc aggcgcactt gaccctgcgg cattttacaa cacagcaggg cggaaacccc
     8221 tccgccgcca cgcagtctgt ctggctcaaa cgcgccgcgc tgctcagtgc cgcggccgac
     8281 tggcgcggcc tcctcgagat ggcggaggag ctgcgggccg tcccgcagac ggctcgggca
     8341 ctgggtggct ggacggactt catcgaggga cgggcgcatc acatgttgaa gcaccacgat
     8401 caggccgacg cgtcaatgca ggcggcagtc cgcgggttct ttccctcaga ccgagtcgcg
     8461 ctcgaggtcg gtcttggctt gaaggaactg ggttattacg acaaggcatg gcaggtgttg
     8521 gcgcccctcg aacagccttt gaaagacgtg ccccactatt ggcaggtcgt tttcgagctt
     8581 gccgaggcca tgcgccagga ccccgtcctg ctcctgagag ccgcaaccga agcccgccgc
     8641 ctccttccgg acgatgaccg atgggatttc aactacgcgg cggcgctgct gatcaacagg
     8701 ctcgatccgg ccagggtggt gagcgtgacg cgcgatttcc ttgtccgcat gcccgcctcg
     8761 ccccacgcgc gcgtcaacca ttgctatgcc ctcgccttga accagcgctg gaacgaagcg
     8821 gccgaactgc tgagcaccgt ggaccccgcc cgttttgacg agtttggacg cagcgtcttc
     8881 cacctctgcg ctttcgagat ccatctcgcg cagggccaga cgaacctcgc gcgcctcgat
     8941 gccgccgaga ttcggcgcga gcacctgttc cccaatcaaa tccgatggct cgatgcagct
     9001 ctgagggaca ccggattcgc ggatctcatc cccccgagat agcttgccga acgcggaggc
     9061 ggggcggcgg gctcagcggc ggcgccgtca tcggtggagt tgcttctcga gatagctcac
     9121 cacctgccgc acgctcgggt ccacctccat gcgcccgcgc accagacggc aggcgtgcag
     9181 aacggtgcca tggtcccggc ctccgaaggc ctcgccgatc gtgttcaagg aactctcggt
     9241 cagttgacgg gccagataca tggccacttg cctggggaaa gcgatgttct cgggccgacg
     9301 ccggctggtc atgtccgcca gccggatgtc gtaatgctca gccactctct tctggatgat
     9361 ctcgatcgtg atcgcgtggc gcccttcctc gtgcagcaac tcgcgaagct gatgttccac
     9421 aacctcgagg gtgacgggct tgcccgccag cgaggcgtag gtggccacgc ggatcaaggc
     9481 gccttccagc cggcgaatgt tcgagcgaat gcgctgggcg agaaaatcga tgacctcctg
     9541 cggcaggctc acccccaggc tctgctcctt cttgcgcaaa atggccacgc gcgtctcgac
     9601 atccggcggc tgcagatccg tcaccaaccc ccactcgaac cgcgacatga gccggtgctc
     9661 gagattctga atctcgctgg cggggcgatc gcacgtcaga acgatctgcc gatgagcctc
     9721 gtgcaacgca ttgaacgtgt ggaagaactc ctcctgaatc cgctccttgt tcgccagaaa
     9781 ctgcacatcg tcgatcagca gcacgtcggt catccgatac ttcttccgaa agcgcaccaa
     9841 ctgattggtc tggatggcgt cgatatactc gttggtgaac ttctccgaag agagataggc
     9901 gacgcgggcg ccctttcgat tctgggccgc atgctgcccg atcgcgtgca aaaggtgggt
     9961 cttccccaaa ccggtccccc catacacaaa gagcggattg taggaccgcc ccggcgcttg
    10021 cgcgaccgcc aatgccgccg catgggcgaa gctgttgttg ctccccacca caaaggtgtc
    10081 aaacgtgttg ttgggattga aaggtgactc gcgcccaacg gcgccggcaa tcggaccgtg
    10141 cgcggctgac accgcgaccc gctgaggtgc cgcctcttcg ttcggcgtcg gctcggctgg
    10201 tttggcggca atgacgaatt ggacacgccg agtcgatccc gtcacgtgcg ccaccacatc
    10261 ctgcaacagg cccaggtaat tgtccttgag ccacagttcg cagaaatcat tcgggacctc
    10321 gactacgatt ccctcctctg acatctccct ggctcgcaag tgggtgaacc agagattata
    10381 gatgtccgca ttgagcatgc ctcgaagaat cgactgagcc gaagaccaga ccttctcgat
    10441 tgaagtttgc atgagacgac aacggccctg acctgcgccg gagacgattt tattcaccac
    10501 cacttgcaac gagcgcgtaa cgtcccgttt tccagaggct tagcacctct ggcattaaaa
    10561 agaattcgga cacctccgtc ggaaaacgcc aaaaaactgg ccaatctcga ctttgaaagt
    10621 cgcaacacat ttcatgccaa cactttacag tcatgtgctg cattgtctct gaggcgctca
    10681 cggaaataat aatccccact cgatccttac tcaggttacg cgacagcgtt tttaggcaag
    10741 acgtccatcg aaaaccagaa caacatatta actcttgtcc acgtgttatt cacaacccgc
    10801 tcttgctccc aaatccaact cgtctcccaa cagcacgccc atcgctttac agccgttccg
    10861 acatcgtccc ccaacgcgta gaccgagcct gttcgcgcag ccggagcggc ggcggattca
    10921 cccagacgga ggcgcccgcg ccctcgaccc gatcgcattt ctccattcgg gctctgcggg
    10981 ctaaacggcc tcctcgtcct tctcgccagt ccggatgcga atggcgttct cgacggagct
    11041 cacgaagatc ttgccgtcgc caatcttgcc ggtcttcgcc gccttcagga tggcctcaac
    11101 agcctgctcg accagctgag ccgcgatcac cagctcgatc ttgatcttcg gcaggaaatc
    11161 gaccgtgtac tcgctgccgc gatagatctc cgtgtggccc ttctggcgcc cgaaaccctt
    11221 cacctcgctc acggtcatcc cctcgatgcc catctgcgcc agcgcctcct tcacgtcttc
    11281 cagcttgaag ggctt
//
LOCUS       DAOQRO010000071        11279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_4949_length_11279_cov_5.380613, whole genome shotgun sequence.
ACCESSION   DAOQRO010000071 DAOQRO010000000
VERSION     DAOQRO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_11279_cov_5.380613"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKK20_04165"
     CDS             <1..827
                     /locus_tag="PKK20_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNU99113.1"
                     /translation="DSEAGRNPDEGPVRRVTIAPFWMGRFEVSWNEYELFMFPDDEKK
                     LRGDQPTEESVDAVSDAVTRPSKPYMEMSFGMGKEGYPAISMTQHAANKYCHWLSAKT
                     GHFYRLPTEAEWEYACRAGTSTAYSFGDDEGKLGEYGVFEDNSDFKYGKIGRKKPNPW
                     GLHDIHGNVAEWCLDQYEDSYESLKDTLINPWNKATKPYPHVVRGGSFDDSASRLRSA
                     ARRASDRNWKMRDPQLPKSIWWLTDAQFVGFRIVRPLEVPSAAQLQKYWTSGVEKE"
     gene            912..1805
                     /locus_tag="PKK20_04170"
     CDS             912..1805
                     /locus_tag="PKK20_04170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNU99114.1"
                     /translation="MRLSFIAIVALCGIGVAASAADDASQLKNSKEKFSYSLGMSLGT
                     NLKRNKLSNDQFDIELIYRGLKDVAGGLPTLLDEAQMRQAIMDYQKEMRTQLGAKNKE
                     AGAKYLAENRTKEGIKTHSVTVATNTYELQYKVLAEGNGQSPSTNDMVTVNYRGTLID
                     GTEFDSSYKRGQPATFPVTGVVRGWSEALKLMKPGAKWELYLPPELAYGEPGTASIEP
                     NSTLIFEIELISAQPRQTPQAASAAQQPVTSDIIKVPSKEELEKGAKIEVIKAADLEK
                     LQAAERAKAEAEKAKDQTDKK"
     gene            2339..4531
                     /locus_tag="PKK20_04175"
     CDS             2339..4531
                     /locus_tag="PKK20_04175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNU99115.1"
                     /translation="MGKKRTDPHQLDLSIQGARASDAAPRPGGLAPAPAAADNATRQD
                     RDAEDKADSKALPSSADSHHETIAEAVSANPSHGSFDPKRIDLPFHRRVDTSFLEYAS
                     YVIRDRAIPALADGLKPVQRRILWAMHQTDDGRFTKVANVIGDTTKYHPHGDASIGDA
                     LVVLANKRYLIERQGNYGNLYTGDPAAAPRYIECRLTELARTELFNDALTAFVPSYDG
                     RKQEPVALPSKLPLALMLGAEGIAVGLSARILPHNFPELLRAQIAILKEQPFKCLPDF
                     PTGGLMDARDYADGKGSVKVRARLRVRDESTIVIKEIPPGTTTESLMASIENAVRKGK
                     LKVKSVHDYTSEEVEIEIKAPAGVDSDKLADALYAFTDCEVTIASRIVVIKDRRPVEM
                     TVSAMLREATDQLCRLLRLELEGKQARLEDELHFRTLERIFIEERIYKKIEQCKTNEA
                     VVSAVYDGFKPFRKECLRALADPDVERLLAVRIRRISLFDINRHRQEMEALKADLAQT
                     RKHLKHLTPYAIAHLEALLERYEPLYPRLTKSSRHDEIDAREVAFRAFKVAYDRKTGY
                     LGYKVNGDEFKVDCTKYDKILMVFKDGRYRMIELPEKLFVGPDVIHAGLPERDRVFTL
                     AYATRDSNYLKRFTFGGAILDKDYWLAPEKSKVLFFEPDTPRELYIRYKPAPYQKINQ
                     QTCDPSQLEVKGAKARGRQISIKDIASITSAPTRGWDAEAPTTRLQFA"
     gene            complement(4825..7596)
                     /locus_tag="PKK20_04180"
     CDS             complement(4825..7596)
                     /locus_tag="PKK20_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNU99116.1"
                     /translation="MARREPGAIEARRGRGDLAGIILFCVALLLFAALFSFDRFDLAA
                     NRVPPNDPAHNLIGRFGASMAYWLFLYFGAGAYLLPVLVLGFAVAHFLSEADYLKRKW
                     PWAILLVLVCAAALDLGTDQAALKRLARNPADTAGFLDRLSLNLSAPSAGGVIGMNLN
                     RYCFRYFGTVGSSILLATLYLVALYGLTNFHLGDWIREYLERRRAARAAAPQNEKMLE
                     RRKRELEREARRLEEKLANAEPAGPAAASPPAPLQARIGLGPDMRPVPEPTVRDLSVP
                     QVGAKPPSRLLPPVKPESPAIEAPADDAVEIIPAREVAASAAGSSAPAPAQAGEGGNS
                     SPTAAASDTPGKEGNGGSIASASGVAKAGGGTGEAAAVTARPRSAMRKAKPLSVATPP
                     LIGNYKLPSIDLLNLPDLAVKPTESKEELMANARLMQQTLAQFGIEVALGDITKGPTI
                     TRYELHPAPGVKLEKITALTNNIAAALKAERIHILAPVPGKSSVGVEVPNAIKSKVIM
                     RELLESDEWRNAKARIPLAIGKDVYGHPIVADLAEMPHLLIAGSTGSGKSVCINAIVA
                     SLLYKFPPDQLRFVMIDPKVVELQQYNTLPHLVVPVVTDPKKVILALRWVVTEMEKRY
                     QIFARVGVRNIRSFNERPKNKPFPVRQPELPLSGRREKVEAGADGFAVEVDEEIVVPR
                     DDDIVIPDKLSYIVVIIDELADLMLVAPADVEMAIARITQMARAAGIHCIVATQRPSV
                     DVITGVIKANIPCRIAFQVAAKVDSRTILDAMGADKLLGKGDMLYLPPGSAKLIRAQG
                     ALVTDQEIQSVVDFIARQGKPLYDVEIHQQLSKPSSSFDDAEGINEEESVIEQCIEVI
                     RSEQKASVSLLQRRLRLGYTRAARIMDELENRGIVGPSKGAEPRDILIDLDVGEESDD
                     DGAGLGSMA"
     gene            complement(7722..8876)
                     /locus_tag="PKK20_04185"
     CDS             complement(7722..8876)
                     /locus_tag="PKK20_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNU99117.1"
                     /translation="MRFEFATADRILFGGGTVREAGAIAKSLGARALVVTGGQTERAA
                     PLLEALHAGGVAMCTFPVSGEPTLDAVMEGRAIARTEACDLVIGMGGGSALDAAKAIA
                     AMCTNEGDLLDYLEVIGRGLPLQRPSAPCLAIPTTAGTGAEVTRNSVIASTAHRVKAS
                     LRSPWILPRVALIDHELTRSLPPGITAATGLDALTQLIEPYVSIRATPLSDGFCVEGI
                     RRAVRSLHRAWENGADATAREDMALASLLSGLALANAGLGAVHGFAAPLGGRYAAPHG
                     AVCAALLPHVMAANLRALRQRQPDGEALRRYDEIGRLLTGRPQAVADDGVAWVRELCR
                     DLRIRPLARHGLASAEIPTVAAQAAQSSSMKANPIPLTATELEQTLLAAL"
     gene            complement(8885..9205)
                     /locus_tag="PKK20_04190"
     CDS             complement(8885..9205)
                     /locus_tag="PKK20_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNU99118.1"
                     /translation="MLVVHVHVHVKPEGVEAFKEATLDNARQSVREPGIARFDILQQA
                     DDPTRFVLVEAYRTADAPARHKETAHYATWRDTVAPLMSEPRHSVKFANVFPPDAGAA
                     EIED"
     gene            9340..9621
                     /locus_tag="PKK20_04195"
     CDS             9340..9621
                     /locus_tag="PKK20_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99119.1"
                     /translation="MRMARIKVSGWGAVYHCISRVVGGLALGQVLRLRVRCSSDGMVL
                     GSPDCVNEIFSEYRDRFGPKRRTGARPMRGLPALENLATMRDLQVDVAS"
     gene            9704..>11279
                     /locus_tag="PKK20_04200"
     CDS             9704..>11279
                     /locus_tag="PKK20_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99120.1"
                     /translation="MLAIISDLHVQETRFDCVRYRANGQLKQIGVRRNVKAAAFKRLV
                     TRIREAARRRKSSEVHLVFAGDVFELHRAPSWFFGSDNHVRPTDAIGEDVPDNPLRRK
                     VSEILTQLETDEDHRQLWKAIRSFASNRPNRRLQVCVHYLPGNHDRLANAWPTVRRQV
                     RRMLGMDDSSSAPFPVCLDFTGPALGDYGVRVRHGHEYDPPNFPWSTSTQTALDRGWQ
                     DYLRPAFGDYVTVDVAMRLALAFRARYAQALRGLVKRNETLPAPDQMRRLYLALTEFD
                     DVRPASLLTEYLAAQMGRGRKETFKALKPVLRDVVETAVANRFFLDEASKHVPAVLLK
                     LLPGLVKNLSAPMLEDIVRTLSKASDKQTDSPAQRALEEFRQNAGLQLVISGHTHSPE
                     QVPLRGGAVPPGVEAFYLNSGTWRTTLPHGVGGFGRLRASTMIFCYNTREQQEGPEDG
                     RTFEVWTGHLAAGTLGPYDEAVQRHSPARSGAMQLVFESFEALAVQKELDGAELKVSF
                     GVDEQGDEAEFEGIRAG"
BASE COUNT         2154 a         3680 c         3586 g         1859 t
ORIGIN      
        1 ccgactcgga agctggccgc aacccggacg aaggcccggt gcgccgggtg acgatcgcgc
       61 ctttctggat ggggcggttc gaagtgagct ggaacgagta cgagctgttc atgttccccg
      121 acgacgagaa gaagctgcga ggcgatcagc ccaccgagga atcggtggat gcggtctcgg
      181 atgccgtcac gcggccgtcg aagccctaca tggaaatgag cttcggcatg ggcaaggagg
      241 gctatccggc catctcgatg acccagcacg ccgccaacaa gtattgccac tggctcagcg
      301 ccaagaccgg ccacttctac cgcctgccca ccgaagccga atgggaatac gcctgccggg
      361 ccggcaccag cacggcctac tcattcggag acgacgaggg caagctgggc gagtatgggg
      421 tattcgagga caacagcgat ttcaagtacg gcaagatcgg caggaaaaag cccaatccgt
      481 ggggcctgca tgacatccac ggcaacgtgg ccgaatggtg cctggaccag tacgaggaca
      541 gttacgagag ccttaaggac accctcatca acccctggaa caaggcaacc aagccgtatc
      601 cgcacgtcgt gcgcggcgga tcgttcgacg actcggccag caggcttcgc agcgccgccc
      661 gccgtgcttc ggatagaaac tggaagatgc gcgacccgca gttgcccaag agcatctggt
      721 ggctcaccga cgcccaattc gtcggtttcc gcatcgttcg ccccctcgaa gtgccctccg
      781 ccgcccagct ccagaagtac tggaccagcg gcgtcgagaa agagtaaccg gcgtgatttg
      841 aattgaattg aattgaattg aaaatcggcg gtggaatctc gaaagtcaac taaaagaaag
      901 agagacataa catgagactt agcttcattg cgatcgtggc cctctgcggc atcggcgtgg
      961 cggcttccgc ggccgacgat gcatcccaac tcaagaactc gaaggagaaa ttctcctaca
     1021 gcctgggcat gagcctgggg accaacctca agcgcaacaa actcagcaac gatcagtttg
     1081 acatcgaact gatctaccgc ggtctgaagg acgtggccgg cggtttgccc acgcttctgg
     1141 atgaagcgca gatgcgccag gccatcatgg attaccagaa ggagatgcgc acccaactgg
     1201 gcgccaagaa caaggaggcc ggcgcgaaat acctggctga gaatcggacc aaggaaggga
     1261 tcaagacgca cagtgtcacc gtggccacca acacctacga actgcaatac aaggtcctgg
     1321 ccgagggcaa cgggcaatcg cccagcacca acgacatggt cacggtcaat taccgcggca
     1381 cgctcatcga cggcaccgag ttcgacagtt cgtacaaacg cgggcaacct gccaccttcc
     1441 ccgtcaccgg cgtcgttcgc ggctggtccg aggcgctcaa gctgatgaag ccgggcgcca
     1501 aatgggagtt gtacctgccg cccgagctgg cctatggcga gcccggcacg gccagcatcg
     1561 agcccaactc gaccctcatc ttcgagatcg aactgatttc cgcccagccg cgtcagacgc
     1621 cccaggcggc ctccgcggcc cagcagcccg tcaccagcga catcatcaag gtgccgtcca
     1681 aggaagaact cgaaaagggc gccaagatcg aagtgatcaa ggccgctgat ctcgagaaac
     1741 tccaggccgc cgaacgcgcc aaggccgaag ccgaaaaggc caaggaccag acggacaaga
     1801 agtaatagcc accgccggct tgtccgcccg atttcgtccg ccgccaagcc gcctgagccc
     1861 ggacatttca cagcgctgcg cgcgatcttc ggatgtccgg gccgcggcaa aaccgcgccc
     1921 gagcctacgg gaggagcaac cttcgccaag tcggctggag tggcgacgtc ctcgtccccg
     1981 gcaaacagaa tcgcagaatc tgttcgcggg cgatcaggac ctgccttccg gcaggcggac
     2041 gtcgccgctc cggcgcggag gttggcacag gccgtggtgc accggttgcc cgtcgctttt
     2101 cgacgacgat ggctggccac gtccggcgca aagcgaacgc ggtgctgagc accgctctca
     2161 gacgtttcgc attgtcggga atgatcgcgc ccaagcagct ttgcgggatg tccgcatccc
     2221 gaccggccct tgggttgacg cgcaagcgca gccgcgggag cgtctttccg gcttttgggt
     2281 tcagcagcga ctcgctcaca gcccggcaat gtcaggcctt tgaacgacac actcgcgcat
     2341 ggggaagaag agaaccgatc cgcaccaact ggacttgagc atccaaggcg ctcgggcgtc
     2401 cgatgccgcg ccgcggccgg gcggcctcgc cccggcgccc gcagcggctg acaatgccac
     2461 gcgccaggat cgggacgccg aggacaaagc ggactcgaag gccctgccga gctcagccga
     2521 cagccatcac gagaccatcg ccgaggccgt ttcggcgaac ccctcccacg gctcgttcga
     2581 tccaaagcgg atcgatcttc cgttccaccg tcgcgtcgac acgagctttc tggagtacgc
     2641 gtcgtacgtg attcgcgacc gggcgattcc ggcgctggcg gacgggttga agccggtgca
     2701 acgccgcatt ctctgggcga tgcaccagac cgacgatgga cgattcacca aggtcgcgaa
     2761 cgtcattggc gacaccacca agtaccatcc gcacggagac gcatcgattg gagatgcgct
     2821 ggtggtgctg gcgaacaagc ggtatctgat cgaacgccaa ggcaactatg gcaacctgta
     2881 caccggcgac ccggccgcgg cgccgcgcta catcgagtgc cgcctgaccg aactggcgcg
     2941 gacggagctt ttcaacgacg cgttgacggc gtttgtaccc agttatgacg gacgcaagca
     3001 ggagccggtc gcgctgccat cgaaactgcc gcttgccctg atgctcggcg ccgagggcat
     3061 tgccgtcggc ctttcggcgc gcattctccc gcacaacttt cccgagctgc tccgggccca
     3121 gatcgccatc ctcaaggaac agcccttcaa gtgcctgccc gacttcccga ccggcggtct
     3181 gatggacgcg cgcgattacg ccgacggcaa gggcagcgtc aaggtccgcg ccaggctccg
     3241 cgtcagggac gaatccacca ttgtcatcaa ggaaatcccg cccggaacca cgaccgaatc
     3301 gctcatggcc tcgatcgaga acgccgttcg caaaggcaaa ctcaaggtca aatcggtcca
     3361 cgactacacc tccgaggagg tcgagatcga aatcaaggcc cccgcgggag tggattcgga
     3421 caagctggcc gacgcgctct acgcgttcac cgactgcgag gtcacgatcg caagccggat
     3481 cgtcgtcatc aaagaccgtc gcccggtcga gatgacggtc tccgccatgc tgcgcgaggc
     3541 taccgaccag ctctgtcgcc tgctccggct cgaactggaa gggaagcagg cccggcttga
     3601 agacgaactc cacttccgca ccctcgaacg gattttcatc gaggagcgca tctacaagaa
     3661 gatcgagcag tgcaagacga acgaggcggt cgtgagcgcc gtctacgacg gtttcaaacc
     3721 gttccgcaag gaatgcctgc gcgccctcgc cgaccccgac gtcgaacgtc tgctggccgt
     3781 gcgcatccgg cgcatttcgc tcttcgacat caaccggcac cgccaggaga tggaggcttt
     3841 gaaggccgat ctcgcccaga cccgcaagca cctcaaacac ctcaccccct acgccatcgc
     3901 gcacctcgag gccctgctcg aacgctacga accgctctac ccgcgcctga ccaaatccag
     3961 ccggcacgac gagatcgacg cgcgggaggt ggcgttcagg gcgttcaagg tcgcctatga
     4021 tcgcaagacc ggttacctcg gctacaaggt caacggagac gagttcaagg tcgattgcac
     4081 caaatacgat aagattctga tggtgttcaa ggatggacgc taccggatga tcgagctgcc
     4141 ggagaagctg ttcgtcgggc cggacgtgat ccacgccgga ctgcccgagc gcgaccgcgt
     4201 ctttaccctc gcctacgcca cgcgcgactc gaattacctg aagcgattca ccttcggcgg
     4261 ggccattctc gacaaggact attggctcgc gcccgagaaa tccaaggtcc tcttcttcga
     4321 gcctgacacg ccgcgcgaac tctacatccg ctacaagccc gccccctatc agaagatcaa
     4381 ccaacagacg tgcgacccgt cgcaactcga ggtcaagggc gccaaggcgc gcggacgcca
     4441 gatctcgatc aaagacatcg cttcaatcac cagcgcaccc acgcgcgggt gggatgcaga
     4501 ggctcccacc acccgactgc agttcgccta gccaataccg cgagcattgc gtagaaggtc
     4561 ttccgatgca ccgaagggcg tcatcgcaaa gcggctatga atcgccgcac tgcgtcgtcg
     4621 gggtgactgg agggtggccg tcctcggcca cagcaatgac ggaatgcacc atgatgtgga
     4681 cgagaccgac gcgtgcggtt gctcggacac cgctgcgccc gaggacgggc gcactccgct
     4741 gggcggcctc ggtctccagt tggctccacc tccctgcgac aaggtcacgg ggtcaagcgc
     4801 ctggccagcc gccagaggcg ggcctcacgc catcgacccc aggccggcac cgtcgtcgtc
     4861 cgactcctct ccgacatcga ggtcgatgag gatgtcgcgg ggctcggcgc ccttgctggg
     4921 gccgacgatg ccgcgatttt cgagttcatc catgatgcgg gcggcgcgcg tgtaaccgag
     4981 acgcaagcgc ctttgcagga gcgacacact ggccttctgc tcgctgcgga taacctcgat
     5041 gcactgctcg atcacgcttt cctcctcatt gatgccttcg gcatcgtcaa aggaactgct
     5101 cggtttcgaa agctgctggt ggatctcgac gtcgtaaagc ggcttgccct ggcgggcgat
     5161 gaagtcgacc acgctttgga tttcctgatc ggtcaccagc gccccttgcg cacgaatcag
     5221 cttggccgag ccgggcggca gatagagcat gtcgcccttt cccagcagct tgtcggcgcc
     5281 catcgcatcg agaatcgtcc tcgaatccac cttggccgcg acctggaaag cgatgcggca
     5341 gggaatgttc gccttgatca cgccggtgat cacatccacg ctcggccgct gcgtggccac
     5401 gatgcaatgg attccggccg cacgagccat ttgcgtgatg cgcgcgatgg ccatctcgac
     5461 gtcggcggga gccaccagca tcagatccgc cagctcgtcg atgatcacca cgatgtaact
     5521 cagcttgtcc ggaatcacga tgtcatcgtc gcgcgggaca acgatctcct cgtcgacctc
     5581 gacggcgaat ccatccgcgc cggcctcgac tttttcgcgg cggccgctca agggaagctc
     5641 aggctggcgg acgggaaaag gtttgttctt gggacgctcg ttgaaggacc ggatgttgcg
     5701 cacaccgacc cgggcgaaaa tctggtagcg tttctccatc tcggtgacca cccagcgcag
     5761 ggccaggatg accttcttgg gatcggtcac caccggtacc accaggtgcg ggagcgtgtt
     5821 gtattgctgc agctcgacca ctttcgggtc gatcatcacg aagcggagct ggtcgggggg
     5881 gaacttgtag agcagcgagg ccacgatggc gttgatgcaa acggacttgc ccgagccggt
     5941 gctgcccgca atcaggagat ggggcatctc cgccaaatcc gccacgatgg gatgtccgta
     6001 gacgtccttg ccaatcgcca gcgggatgcg cgccttcgcg ttgcgccact catccgactc
     6061 gagcaactcg cgcatgatca ccttggactt gatcgcgttg ggcacctcga cgccgacgga
     6121 actcttgccc ggcaccgggg cgaggatgtg aatgcgttcg gccttgagcg ccgcggcgat
     6181 gttgttggtc agagcggtga tcttctcgag cttgactccg ggcgccggat gcagctcgta
     6241 gcgcgtgatg gtcggcccct tggtgatgtc gcccagagcc acctcgatgc cgaattgggc
     6301 cagggtctgc tgcatcagac gcgcgttggc catcaactcc tctttcgact cggtcggttt
     6361 caccgccaaa tcgggcagat tcagcagatc gatggacggc agcttatagt tgccgatcag
     6421 cggcggcgtc gccaccgaca gcggcttggc cttgcgcatg gccgacctcg ggcgcgccgt
     6481 caccgccgca gcctcgcctg tccctccccc cgccttcgcc acgcccgacg ccgacgcgat
     6541 agaaccgccg ttgccctcct tgccaggcgt gtccgatgcc gctgcagtcg gactcgaatt
     6601 cccgccctcc ccggcctgtg ccggcgcggg cgcactggac cccgccgcgg atgccgccac
     6661 ctccctggcc gggatgatct cgaccgcatc gtcggcaggc gcctcgatcg ccgggctctc
     6721 gggcttgacg ggcggcagga ggcgtgaggg cggcttggcg cccacctgcg gcacgctcag
     6781 atcgcgcacc gtgggttccg gcaccggacg catatcgggc cccaatccga ttcgggcctg
     6841 cagaggagcc ggcggagacg cagccgccgg tccagccggc tcggcgttgg caagcttttc
     6901 ctcgaggcgg cgcgcctctc tctccaactc ccgtttgcgc cgttcgagca tcttctcgtt
     6961 ctggggcgca gcggccctgg cggcgcggcg cctctcgaga tactcgcgga tccagtctcc
     7021 cagatggaag ttcgtgaggc cgtacagcgc gacaaggtag agcgtcgcca gcagaatcga
     7081 ggagccgacc gtgccgaaat agcggaagca gtagcgattg aggttcatcc cgatcacccc
     7141 gcccgcgctc ggcgcgctca ggttcaacga gaggcggtcg aggaaccccg cggtatccgc
     7201 cggattgcgg gcgagccgct tcaaggccgc ctgatcggtt ccaaggtcca gggcagcggc
     7261 gcagacgagc accagcagga tggcccaagg ccacttgcgc ttgaggtaat cggcctcgct
     7321 cagaaaatgg gccaccgcaa agcccagcac cagcacgggc agcaaatagg cgcccgcccc
     7381 gaagtagaga aacagccagt acgccatcga cgcgccgaag cgcccgatca ggttgtgagc
     7441 cggatcgttg ggcggcacgc ggttggcggc cagatcgaat cggtcaaacg aaaacaacgc
     7501 ggcgaacaaa agcaacgcca cgcaaaacag aatgatgccc gccaaatcgc cccgaccgcg
     7561 tctggcctcg atcgcgccgg gttctctacg cgccatgcgt ccactctaag aaacgcccgc
     7621 cgaagttcaa tgtggaaagt ccgggcggtg ccaaccccca tattccgctc cccgtcagcg
     7681 cacaggacgc cttggacctt gcagacaacg cgccgacacc gttagagggc cgccaggagt
     7741 gtctgctcga gctcggtcgc cgtcaggggg atcggattgg ccttcatgct gcttgactgg
     7801 gcggcttgag ccgccacggt cggaatctcg gccgacgcaa gcccgtgccg cgccagcggc
     7861 cggattctca ggtcgcggca caactcccgg acccacgcga cgccgtcatc ggcgacagct
     7921 tgaggacgcc cggtcagcag acgcccgatt tcgtcgtagc gccgcagggc ctcgccatcc
     7981 ggctggcgtt ggcgcagggc gcgcaggttg gcggccatga cgtgaggcag gagcgccgcg
     8041 cagaccgccc catgcggggc ggcatagcgt ccgccgagcg gggcggcgaa gccgtgcacg
     8101 gccccgaggc ctgcgttcgc cagggccagt ccgctcagga ggcttgccaa ggccatgtct
     8161 tcgcgcgccg tggcgtcggc cccgttctcc cacgcccggt gcaaggaacg aacagcccgg
     8221 cggattccct cgacgcagaa accgtcggac aaaggcgtgg cgcgaatcga cacgtagggc
     8281 tcgatgagtt gtgtgagagc gtccaaaccg gtcgcagcgg tgatcccggg cggaaggctg
     8341 cgcgtcaact cgtgatcgat cagcgcgaca cgcggcagaa tccagggact gcggaggctg
     8401 gccttgacgc ggtgggcggt cgaagcgatc acgctgttgc gcgtcacctc cgcgcccgtg
     8461 ccggccgtgg tggggatcgc gaggcagggc gccgatgggc gttgcagcgg cagaccgcgt
     8521 ccgatgactt cgaggtaatc gagcagatcg ccctcgttgg tgcacatcgc tgcgatggcc
     8581 ttggccgcgt cgagcgcact gccgccgccc atgccgatga ccaggtcgca cgcctcggtg
     8641 cgggcgatgg cccgcccttc catgaccgcg tccaaggtgg gctcgccgga aacggggaaa
     8701 gtgcacatgg caaccccgcc ggcatggagg gcttctagga gcggcgcggc tcgctccgtt
     8761 tggccgccgg taacgaccaa ggcgcgcgcg ccgagcgact tcgcgatggc gccagcctcg
     8821 cgcacagttc cgccgccaaa cagaatccga tccgccgtcg caaattcgaa gcgcatggaa
     8881 ttcttcaatc ttcaatctcc gccgccccgg cgtcgggagg aaacacattg gcgaacttca
     8941 cgctgtggcg gggctcgctc atcagcgggg cgacagtgtc ccgccacgtc gcatagtggg
     9001 ccgtttcctt gtgccgggcg ggggcgtcgg cagtgcgata ggcctcgacc agaacgaacc
     9061 gggtcgggtc gtccgcctgt tgcaggatgt cgaatcgagc gatcccgggt tcgcgcacgc
     9121 tctggcgcgc gttgtcgagc gtggcctctt tgaaggcctc gacgccttcg ggcttcacgt
     9181 ggacatggac atgaacgaca agcatgggag cagtattcag atgggacggc ccgcctggca
     9241 actcgaagtt gcggctcaca aagcgagagg ctgttcgtga cagacggcgt gtttgagggt
     9301 aaacacacgc aggaacacgg cgatgaccca tcgaatccca tgcgcatggc gcgaatcaaa
     9361 gtgagcggct ggggagcggt ctaccactgc atctcccggg tggtgggcgg cctggccctg
     9421 ggtcaggtgc tgcgattgcg ggtgaggtgc tcgagcgatg gaatggtgct gggatcaccg
     9481 gactgcgtca acgagatctt cagcgaatac cgtgatcggt tcgggccgaa gcggcgaacc
     9541 ggggctcgac cgatgcgcgg tttgcccgcc ctcgagaatc tggcgacaat gcgggacctg
     9601 caagtcgacg tggcaagttg agagagccgg gtacgggcgt cggggtgtgc cgccggctga
     9661 ttggcgtgag gagcgagcgc cggcgcggct cgcgcagtcg agtatgcttg cgattatcag
     9721 cgatctgcat gttcaggaaa cgcggtttga ctgcgtccgc taccgggcga acggccaact
     9781 caagcagatc ggtgtgcggc gcaatgtcaa ggcggccgcc ttcaagcggc ttgtcacgcg
     9841 gattcgggag gccgcgcgac ggcggaagtc ctcggaggtg catctggtgt ttgccggcga
     9901 cgtcttcgaa ctgcatcggg cgccgagctg gttcttcgga tcggacaacc atgtgcgccc
     9961 gaccgatgcg atcggcgagg atgtcccgga taatccgctt cgacgcaagg tctcggagat
    10021 tctcacccaa ctcgagaccg acgaggatca tcggcaactc tggaaggcga ttcggagttt
    10081 cgcttcgaat cgcccgaacc gtcggctgca ggtctgcgtc cattacctgc cgggcaacca
    10141 cgatcgtctt gccaatgcct ggcccaccgt gcggcgacag gtgcgccgga tgctgggcat
    10201 ggacgacagc tcgagcgcgc cctttcccgt ctgcctcgat ttcaccggcc ctgcgctggg
    10261 ggattacggc gtgcgagtcc gccacggcca cgaatacgat ccgcccaact tcccatggtc
    10321 cacgtcaacg cagacggctt tggaccgcgg ctggcaggac tatctcaggc cggcgttcgg
    10381 cgactatgtc actgtggatg tggccatgcg gctggcgctc gcgtttcgcg cccgttacgc
    10441 ccaagccctg cggggtctgg tgaagcggaa cgaaacgctg cctgcgcccg atcagatgcg
    10501 ccggctctat ctggccttga ccgagtttga cgacgtgcgc ccggcatcgc tgctgaccga
    10561 gtacctggcc gcgcagatgg ggcggggccg caaagagacg ttcaaggctc tgaaaccggt
    10621 cctgcgcgat gtggtcgaga ccgccgtggc gaatcggttt tttctggatg aggcttcgaa
    10681 gcacgtgcct gcggtgcttc tcaagctgct tccgggcctg gtgaaaaacc tctccgcccc
    10741 gatgcttgaa gacatcgtgc gcacgctctc gaaggcctcg gacaaacaga cggacagccc
    10801 ggcgcaacgc gctctcgaag agttccgcca gaacgccggc cttcagcttg tgatcagcgg
    10861 ccacacgcac tctcccgagc aggtcccgct gaggggcggg gccgtgcctc cgggtgtcga
    10921 ggcgttttac ctcaacagcg gcacctggcg caccaccctc ccccacggcg tcggcggctt
    10981 tggacgattg cgcgcctcga cgatgatctt ctgctacaac acccgcgaac agcaggaagg
    11041 tccggaggac ggacgcacgt tcgaggtttg gaccggccat ctcgccgccg gaacgctcgg
    11101 gccgtacgac gaagcggtgc aacggcactc gcccgcccgt tcgggtgcaa tgcagttggt
    11161 cttcgagtcc ttcgaagcgc ttgcggtgca gaaggaactc gacggcgcgg aactgaaggt
    11221 gagtttcggc gtggacgagc agggtgatga ggccgaattc gagggcatcc gggctggag
//
LOCUS       DAOQRO010000072        11209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5003_length_11209_cov_5.386229, whole genome shotgun sequence.
ACCESSION   DAOQRO010000072 DAOQRO010000000
VERSION     DAOQRO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5003_length_11209_cov_5.386229"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..956)
                     /locus_tag="PKK20_04205"
     CDS             complement(225..956)
                     /locus_tag="PKK20_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99121.1"
                     /translation="MNLQRVPRMLVSSLAILTVCAWSAFAGDPEEGFTPMFNGKDLTG
                     WEGKPGWWRVENGCITSESTPEKPCPTAHYLMWRGGKPGDFELRLEYRLVGGNSGVQF
                     RSKELPEWDTSGYQADMEDGGQWTGCLFEHTRGGVSMRGERVVIDPDSTRHVTRIGDP
                     DELLKRVKKQDWNSYRIIALGDQIVLEINGTVMCRAIDRQTGLAAKEGVIALQMHPGP
                     PMKVQFRNLRIKLFDPPPSPPQTAR"
     gene            complement(1266..2660)
                     /locus_tag="PKK20_04210"
     CDS             complement(1266..2660)
                     /locus_tag="PKK20_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99122.1"
                     /translation="MKDTLDHLTSRREFLKSTSRVAAASALAGVALPHVHAADDSTLQ
                     LALIGCGGRGTGAIVNALATRNQGPIKLVAMADVFKERLDDSYRNLSRNHAAQMDVPP
                     ERRFIGFDAYKKAMDCLKPGDIMVAATPPAFRWVHFTYAIQKGLHAFMEKPVTVDGPT
                     SRRMFELGEAATKKNLKVGIGLMSRHSRHLAQLAERVHNGEIGDIILQRGYRMHGPVG
                     FFASVPKPAGVSDLMYQVQRFHSFIWASGGNYSDFYIHIIDHLGWIKNAWPVKAQALG
                     GRHYRQSPEGVTYVDQNFDTYAVEYTYADGTKFDFDGRCMTGCMDQYHSYIHGTKGSA
                     IASKNGDCGGPSTIFKGQTPRRSDMLWESKVTPGEEDPYQNEWNDLINAIRNDKPYNE
                     VKHGVEASAVTSMGRMAAHTGQEITYEDFINGDHEMAPGLDQLTESSEAPLKAGPDGR
                     YPVPEPGRKRTREY"
     gene            complement(2826..10310)
                     /locus_tag="PKK20_04215"
     CDS             complement(2826..10310)
                     /locus_tag="PKK20_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNU99123.1"
                     /translation="MRHLGPLNMRRIAREMAGLGRSSLLLAWLVTGSFGTAAHAGGLL
                     REVYTDIPGARLDDLFNSAKYPDRPDLTEILPEFEAPTDVMDNYGQRLRGFLVPPVTG
                     DYVFWIAGDDESVLYLSSTTSPADAVDIAAVPDWTSSREWTKYRSQQSGVIWLDASRM
                     YYVEALMKEGGGGDNLAVRWQLPTGAIEEPIPEARLVPFGTILTPPVIRTQPANQTVI
                     EGQPAVFSVSVNNPDPVRYQWQRGGVAIPGATSASYTLPSVRLSDHGASFRCTITNSL
                     GSALSAAAALSVQPDVTPPLLRQAHNVGARTVVVTFSEPVDYASAAVAGNYRLDQGRV
                     LSAQPQSDARLVSLQVDALEFGRPYLLTVNNLKDRSVAGNLLAPNSQIGFVASILTAS
                     DLGEFPVPGAAVSVPGGFDITAMGTGLGDWTDLLHFSYQERTGDFDVRVRVAAVELTD
                     VWATAGLMARALRSTNGPMAGVFATPSISGVFFASRGATNPLSISPSMPVNYPYTWLR
                     LRRTGNLFTGYSSYDGEAWRTLRSVTNPMPALVYLGLSVSSQQTNRTLLARFREFSDV
                     VSATEAERLPDVESLGPSSRRTPLVISEIMYNPLARADGSNLEYVELFNSQPVAESIG
                     GFRLSGSIDYTFPPNTRIDGGGFVVVAKEPSALEAASGLPRVYGPYKNNLPGNGGRVT
                     LHSELGAVLVDVQYSDQPPWPASADGAGHSLVLARPSLGEGRSAAWSASLMRGGSPGA
                     MEPIRLDAWASIKINEFLAHTDYPALDFIELYNAGARTVNLGGAFLSDSPGVDKFRIP
                     NGTLLPPGGFVQFTQNDLGFALSSSGETIYLVNPDQTQVIDAVRFGPQAKGVASGRYP
                     DGAPGIHELRTPTPGRRNDGLRINEIVINEIMFHPASGDDADEYVELHNRGLHPVDLS
                     HWRFVDGIDFAFPPGVTIPGGGYIVVAKDAARLRSNHPALGAGAVVGDYQGKLANGGE
                     RVALARPSDPSSPLANLIVVDEVAYGDGGRWGQWADGGGSSLELVDARSDNRLPANWA
                     VSDETAKSAWVAVEHTGVLDLGTDVYGVDRLQVLLMGEGECLIDDVQVIGPNGNNLVP
                     NSTFEQGAQGWRARGTHARSSLETTEGYRSGKSFRLRASGRGSTDANHVFIDLTGSLT
                     PGTYATLRARARWCRGNPELLLRLRGNHLEAAGRLPVPLAAGTPGARNSTARANTGPA
                     IWDVKHSPILPAIGQSVLVTARVHDPDGLLAVECLYRIDPSSQTTRLAMRDDGTGGDV
                     LGGDGLYSAMLPPQTTTNLIAFTVEASDAAATRAISRYPVDSPERECLVRWGEQTAVG
                     NLGTYRIWMTRATLSEWETREPSSNDPLPVTFVYGHERVVYEASALYSGSPFHWGGYD
                     SPMGLNCNYVLLFPKDDRFLGATDFVLNLPANLGSDRSAQREQFFFAMARAIGRPHAC
                     RRFCHLIVNGLVRGPIFEDAQQPNRDYVEQWFPDDAEGELFKIEDWFDYDDTILRFQN
                     VDASLDRFTTADGDYKLARYRWNWRKRAVRDSAHDYARLFELVNALNANPSEYTARVE
                     SLVDVDGWMRTFALRHVVGDWDSYGYRRGKNMYAYRPVHGRWQLLDWDVAFAFGLGDG
                     TTHDLFDARHSDGGIDAVTERMYNHPPFRRAYLRALYDIAHGPLDEAWARQWLGSTYT
                     ALADNALAATSPDTILTWIRNRRLHILEMLATNVVPFAVTSNGGIDFTVQGNNLVELE
                     GTAPVQMQTLRVNGRAYPVTWTSVTDWRMDLPLRSGVNDLKFEGYGSTGVLIPGAAAT
                     LRIDYAGPDQNPQDYLVISEVHYHPNVANTGFVEIHNRSDTHAFDLTGFQLRGAGFAF
                     AEGTVIEPGAYLVVVTDRSAFGAEYGYRARVAGVLPGRLRNDGETLSLVRPGATPEQD
                     VVIDRVTYDDAWPWPAAADGEGSSLQLVDPTEDNRRPGAWVAVTNDPSTTGWKFAQIT
                     GIASSGSLYFYLESSGEVFLDDVWLAAGPLAETGTNLVENAGFEQPLAGSWIVSDTDP
                     GSRIESTLSHSGAASMRLSSETGGNILLPSLGQASLAVRAGELYTLSFWYHPTPTGGG
                     LTAGLLGSALELTVSLVPGELPLPLATPGAPNVLPGTFTPLPPLWINEVMPNNLSGPR
                     DRFGDADPWIELFNAGGAPVSLDGLFLAHNGPGLLEWPFPPGLTIEPGERLVVWADGE
                     PDESSAEELHANFRLDPIAGAIALSIQDGAAIRVVDSLRYSDVFPDRSWGSWPEGDPN
                     SRQVLHYPTPGAPNDPSSAPVVVYVNEWMAANRTTVADPADQDTDDWFELYNPGDVAV
                     DLSGYSVTDDLGEPRRWVLPSGTIIPGRGYLLIWADDETEQNGLGLGLHANFRLSQKG
                     ESLGLFAPDGTQVDAVTFGPQEDDVSEGRYPDGAPPPFMAMTTPTPGGPNVAPGPSAP
                     RAISIEFGGAGIAVRWAVEPGQTYRVEFTDDPVVSVWQVVGGPAVATEPVLEFVDPID
                     PWRRQGFYRLVLLP"
     gene            complement(10341..>11209)
                     /locus_tag="PKK20_04220"
     CDS             complement(10341..>11209)
                     /locus_tag="PKK20_04220"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99124.1"
                     /translation="SITETLYSLDLTLPLGGTVSPSSGLYPEGSEVFVNAIPATDYLF
                     AHWEDIGGQVVSTQNPLRLVMDRNYSLIARFRLKNYSDTFGSGDLNRLPWTWSGAKGW
                     SVAMVEGQYVAQSGSIGNGQTSSLILISYLYDGTGAFDLSVSSEEGWDALEFYVDGRR
                     LGRWSGDVLWQTFMFPITEGRRVLEWRYVKDANYSEGLDRAFIDNVYLPLQQPEPPNI
                     RPSIDVVRLANGAVQLTVVCLPNVSYVIEASPNLKAWTAVSTNRPVSAAFQWLDPDTL
                     SRAARFYRAVAR"
BASE COUNT         1888 a         3481 c         3831 g         2009 t
ORIGIN      
        1 tcaggccgta gcggcgcgcg aatccctctt ggctcagccc actacgatgg aaccgatcca
       61 accattgcgc ccgttggtcg gtgggagtcg gagcgctttc acgaggagtg agtttctgac
      121 gagacgatgc attcatcgcc ccaggttaaa ccacttccgc cgccctcgac gctagtgccc
      181 gttcccctga cggatactct tcttcggcct gctctttgga ccgctcagcg ggcggtttgg
      241 gggggcgagg gcggcgggtc aaacagcttg atccggagat tccggaactg gaccttcatg
      301 ggggggcccg gatgcatctg gagggcgatg acgccttcct ttgcggcaag acctgtctgc
      361 ctgtcgatgg cccggcacat cactgtgccg ttgatttcga gcacgatttg atcgccgagc
      421 gcgatgatgc ggtagctgtt ccagtcctgt tttttgacgc gtttgagaag ttcatccgga
      481 tcgccgatgc gtgtgacgtg gcgggtgctg tccgggtcga tgaccacccg ctcgccgcgc
      541 atcgacacgc ctccgcgcgt gtgctcgaat agacagccgg tccattggcc gccatcttcc
      601 atgtccgcct ggtacccgga cgtgtcccat tcgggaagtt ccttgctgcg gaattggacg
      661 cccgagttgc cgccgaccag ccggtattcg aggcgcagtt cgaagtcgcc cggcttgccg
      721 ccgcgccaca tgaggtagtg cgccgtgggg catggttttt cgggggtgct ttcggacgtg
      781 atgcaaccat tctcgacccg ccaccatccg ggcttgccct cccagccggt gaggtccttg
      841 ccgttgaaca tcggggtgaa gccctcctcg gggtcaccag caaaggcgga ccatgcgcag
      901 acggtgagga tggcgagact ggagacgagc attcgaggaa cacgttgaag attcatgggg
      961 aatgataatc tcctttcaaa acactttggg tgcgtgtagc cgccgacgtg agtcggcgcc
     1021 gattcctgcc tttgcggagc gagatggcgc ggactcatcc cgttctccgg gacggctacg
     1081 gaaccacgtt tcattgagaa agccgcggac agacacaacc tgggcacgga acgaaccgca
     1141 ttcgaacagg cgttgtgggc cgatcggccg ggggccggga acggcgctgg acgcactgtc
     1201 tgagctgcga ctcatccgtg ttgcatccgt gtctctccgc ggtcgcgggg tctctcaggt
     1261 ctagtctagt actcgcgggt gcgtttgcgt ccgggctcgg ggacggggta acggccatcg
     1321 ggaccggcct tcagaggggc ttcggagctt tcggtgagct gatcgaggcc gggggccatt
     1381 tcgtggtcgc cattgatgaa atcctcgtac gtgatctcct gtccggtatg ggcggccatg
     1441 cggcccatcg aagtgaccgc gctggcttcg acgccgtgtt tgacttcgtt gtacggtttg
     1501 tcgttacgga tggcgttgat gaggtcgttc cattcgttct gatagggatc ctcttcgccc
     1561 ggggtgacct tggactccca gagcatgtcg gagcgccttg gtgtctggcc cttgaagatg
     1621 gtcgagggtc cgccgcaatc gccgtttttc gaggcgatgg cgctgccctt ggtgccgtgg
     1681 atataactgt ggtactggtc catgcagccc gtcatgcagc ggccgtcgaa gtcgaacttg
     1741 gtgccgtcgg catacgtgta ctcgaccgcg taggtgtcga agttctggtc gacgtaggtg
     1801 acgccttcgg ggctctgccg atagtggcgt ccgccgagcg cctgggcctt gaccggccag
     1861 gcgttcttaa tccagccgag atgatcgatg atgtggatgt agaagtcgct gtagttgccg
     1921 ccgctggccc agatgaaact gtggaaacgc tggacctggt acatcagatc gctcacgccg
     1981 gccggcttgg gcaccgacgc gaagaatccg accggcccgt gcatccggta tccgcgctgg
     2041 aggatgatgt cgccgatctc gccgttgtgg acgcgttcgg ccagttgggc gaggtggcgg
     2101 ctgtgacgcg acatgaggcc aattccgacc ttgaggttct tcttggtggc ggcttcgccc
     2161 aactcgaaca tgcgcctcga ggtcgggccg tcgacggtga cgggcttttc catgaaggcg
     2221 tgcaggccct tctggattgc gtaggtgaag tggacccagc ggaaggccgg cggcgtggcg
     2281 gcaaccatga tgtcgcccgg cttcaggcaa tccatcgcct tcttgtaggc gtcgaatcca
     2341 atgaaccgcc gttcgggcgg cacgtccatc tgagcggcat ggttccggct caggttgcga
     2401 tagctgtcgt cgaggcgttc cttgaagaca tcggccatgg ccaccagttt gatggggccc
     2461 tggttgcgcg tggccaaggc gttgacgatg gcgcccgtgc cgcgtccgcc gcaaccgatc
     2521 agcgccaact ggagtgtgct gtcgtcggcc gcgtgaacat gcggcagggc aacgccggcc
     2581 aacgccgagg cggcggcgac gcggctggtg gatttcaaga actcgcgacg tgatgtcaga
     2641 tgatccaggg tgtctttcat gggcgagatt cttcctgcga gcgcctccgg gagtcaactc
     2701 ccgagatggg agatcggata tggggggtgg acaggcggtg tgcgggcctg tctgcccctc
     2761 gaccgacggc ggtgcagccg gatgctccga ccgagagcgg ccctccgaat cgaggagagc
     2821 gcgttctatg gcagcaacac gaggcgatag aagccctgcc ttcgccacgg atcgatcgga
     2881 tcaacgaact cgagcaccgg ctcagtggcc accgcgggtc cgccgacgac ctgccagacc
     2941 gacacgacag ggtcgtccgt gaactcgact cggtaggtct ggccgggctc gaccgcccag
     3001 cggaccgcaa tcccagcgcc gccgaactcg atgctgatgg cccgcggggc cgaagggccg
     3061 ggcgcgacat tcggtccgcc cggggtcggc gttgtcatgg ccatgaacgg cggcggcgcg
     3121 ccatccgggt accggccctc gctcacatcg tcctcctgag gaccgaacgt gaccgcatcg
     3181 acctgggtcc cgtcaggggc gaacagcccg agagattcgc ccttctggct gaggcggaag
     3241 ttggcgtgga ggccaaggcc gagtccgttt tgctcggtct cgtcatccgc ccaaatcagc
     3301 aggtagccgc ggccagggat gattgtcccg gacggcagca cccatcgacg cggctctccc
     3361 aagtcgtcgg tgaccgaata gcccgagaga tcgaccgcga cgtcgcccgg gttgtagagc
     3421 tcgaaccaat cgtcggtgtc ctgatccgcc gggtccgcca cggtcgtcct gttggcggcc
     3481 atccactcgt tcacgtagac gacaaccggc gccgatgagg ggtcgttggg cgcgccgggc
     3541 gtggggtaat ggagcacctg ccgcgaattg ggatcgcctt cgggccacga tccccaggag
     3601 cgatcgggga atacgtcgga gtagcgcagg gaatcgacaa ctcggatggc agccccgtcc
     3661 tgaatcgaaa gcgcgattgc accggcgatc ggatcgaggc ggaagttcgc gtgaagctcc
     3721 tcggcgctgc tttcgtctgg ctcgccgtcg gcccacacca ccaggcgctc gcccggctcg
     3781 attgtgagtc cgggcgggaa cggccattcg aggaggccgg ggccgttatg ggcgaggaac
     3841 aggccatcga gggagacggg ggcgccgccg gcgttgaaca gctcgatcca aggatcggcg
     3901 tcgccgaagc gatcccgcgg gccgctgagg ttgttaggca taacctcgtt gatccagagc
     3961 ggcggaagcg gcgtgaaggt gcccggcagg acgttgggcg cgccgggcgt ggcgagcggc
     4021 agcggcaatt cgcccggcac gaggctgacg gtcagttcca gcgcgctgcc cagcaggccg
     4081 gcggtcaggc ctccgccggt cggagtcggg tgataccaga agctgagcgt gtagagttcg
     4141 cccgcgcgga ccgccaggct ggcttgtccg agtgagggga ggagtatgtt gccacccgtt
     4201 tccgagctca gccgcatcga tgcggcgccc gaatggctca gggtcgattc gattcgactg
     4261 ccggggtcgg tgtcggatac gatccacgat ccggcgagag gttgttcgaa tccggcattc
     4321 tccaccaggt tggtcccggt ctccgccagc ggcccagcgg ccagccagac atcgtcgagg
     4381 aagacttcgc ccgacgactc gaggtagaaa taaagagacc cgctgctggc gatgccggtg
     4441 atctgggcga acttccagcc ggttgtggac ggatcattcg tgacggccac ccaagcgccc
     4501 gggcggcggt tgtcttcggt gggatcgacc aactggagcg aggaaccctc gccatccgcc
     4561 gcggcgggcc aaggccaggc gtcgtcgtag gtgacacggt cgatgaccac atcctgctcg
     4621 ggtgttgccc cgggccggac gaggctgaga gtttcaccat cgtttcgcaa acgaccgggc
     4681 agcacgccgg caacgcgcgc ccggtatccg tactccgcgc cgaaggcgga ccggtcggtg
     4741 acgaccacca ggtaggcgcc cggctcgatc acggtgcctt cggcgaacgc gaagcccgcg
     4801 ccccgaagct ggaatccggt cagatcgaac gcgtgcgtgt cggacctgtt gtggatctcg
     4861 acgaagccgg tgttcgccac gttcgggtgg taatggactt cgctgatgac caggtaatcc
     4921 tgcgggttct ggtcgggccc ggcgtaatcg atgcggaggg ttgcggccgc gccgggaatc
     4981 agaactccgg tcgagccata tccttcgaat ttcaggtcat tgaccccgct ccgcaaaggg
     5041 agatccattc gccagtccgt aacgctggtc catgtcaccg gataggcgcg tccgttcacc
     5101 cggagggttt gcatctgcac gggggcggtg ccttcgagtt cgacgaggtt attgccctgg
     5161 accgtgaagt cgatgccgcc gttgctggtg acggcgaagg ggacgacatt ggtggcgagc
     5221 atttcgagga tgtggaggcg ccggttgcga atccacgtca ggatggtgtc ggggctggtg
     5281 gcggccaggg cattgtccgc gagggcggtg taggtcgatc ccagccactg gcgcgcccag
     5341 gcttcgtcga gcgggccatg ggcgatgtcg tagagggcgc ggaggtaggc gcggcggaag
     5401 gggggatggt tgtacatgcg ctcggtgacg gcgtcgatgc cgccgtccga gtgtcgagcg
     5461 tcgaacaggt catgggtcgt gccgtcgccc agaccgaagg cgaaggcgac gtcccagtcc
     5521 aggagttgcc accgcccgtg gacggggcga taggcataca tgttcttgcc gcgtcgatag
     5581 ccgtaggaat cccaatcgcc cacgacgtgg cgcagggcga acgtgcgcat ccaaccgtcg
     5641 acgtcgacga ggctttcgac gcgcgcggtg tattccgagg ggtttgcgtt gagggcgttc
     5701 accaactcga agagccgcgc gtaatcgtgc gccgagtcgc gcacggcgcg cttgcgccag
     5761 ttccatcggt agcgggccag cttgtagtcg ccgtcggcgg tggtgaacct gtcgagggac
     5821 gcgtcgacat tctggaagcg caggatcgtg tcgtcgtagt cgaaccaatc ctcgatcttg
     5881 aacaattcgc cctcggcatc gtccgggaac cactgctcga catagtcgcg gttgggctgt
     5941 tgggcgtctt cgaagatggg gccccggacc aggccgttga cgatcaggtg acagaatcgg
     6001 cggcaggcgt ggggtcggcc gatcgcgcgg gccatggcga aaaagaactg ctcgcgctgg
     6061 gcgctgcgat cgctgcccag attggccggc aggttgagga caaaatcggt cgcgccgagg
     6121 aagcggtcgt ctttggggaa cagcaggacg tagttgcagt tgagccccat cggcgagtcg
     6181 tagccgcccc aatggaacgg gctgccgctg tagagcgcgc tggcctcgta caccacgcgt
     6241 tcatggccgt acacaaacgt cacgggcaac ggatcgttgc tgctgggctc gcgcgtctcc
     6301 cattccgaca aggtggcgcg cgtcatccag atgcggtacg tgcccaggtt ccccacggcc
     6361 gtctgttcgc cccagcgcac caggcactcg cgttcgggcg agtctaccgg gtacctgctg
     6421 atagcccgcg tggcggctgc atcggatgcc tcgacggtga atgcgatgag gttggtggtg
     6481 gtctggggcg gcagcatcgc cgagtacagg ccgtccccgc ccaacacgtc gccgccggtc
     6541 ccatcgtccc gcatcgcaag ccgcgttgtt tgcgacgagg ggtcgatgcg gtagaggcac
     6601 tcgaccgcca acaaaccgtc cggatcgtgg acgcgggcgg tcacgagcac ggattgcccg
     6661 atagcgggca gaatcgggct gtgcttgaca tcccagatcg ccgggccggt gttcgcacga
     6721 gcggtgctgt tgcgagcccc gggcgtgccg gcggcgagcg ggacaggcag ccgtccggcg
     6781 gcttcgaggt gattgccgcg caggcgcagc aggagttcgg ggttgccgcg gcaccaccgc
     6841 gccctcgccc ggagtgtcgc gtaggttcca ggcgtcagcg atccggtcag atcgatgaag
     6901 acgtggttcg cgtccgtcga gccgcgtccg ctggcgcgga gccggaagct tttgccgctg
     6961 cggtagcctt cggtggtttc gaggcttgac cgggcatgcg tgccgcgcgc gcgccaacct
     7021 tgggcgccct gctcgaaggt cgagttgggg accaggttgt tgccgttggg cccgatcact
     7081 tggacatcgt cgatcagaca ttcgccctcg cccatgagga gaacctgcag ccgatccacg
     7141 ccgtacacgt ccgtgccaag gtccaggacg ccggtgtgct cgacggcaac ccacgcgctc
     7201 ttggctgtct catcgctcac ggcccagttg gccggaaggc ggttgtcgct gcgcgcgtcc
     7261 acaagctcga gactgcttcc gccgccgtcg gcccattgtc cccatcgccc gccgtcgccg
     7321 tacgccactt catccacgac gatcagattc gccaaggggc tcgacggatc actcgggcga
     7381 gccagggcca cgcgttcgcc gccgttggcg agcttgccct ggtaatcgcc cacgaccgcg
     7441 cccgcgccga gcgcgggatg gttggatcgt aaacgcgcgg cgtccttcgc cacgacgatg
     7501 tagccgccgc ccggaatcgt cacgccgggc gggaatgcga aatcgatgcc gtcgacgaat
     7561 cgccaatgtg agagatcgac ggggtggagg ccgcggttgt gaagctcgac gtattcgtca
     7621 gcatcgtcgc cgctagccgg gtggaacatg atctcgttaa tcacgatctc gttgatgcgc
     7681 aggccgtcgt tgcgccggcc gggcgtcggc gtccgcaact cgtggatacc gggcgcgccg
     7741 tcggggtatc ggccgctggc gactcccttg gcctgagggc cgaagcgcac tgcgtcgatc
     7801 acctgggtct ggtcggggtt gacgaggtag atggtttcgc cggaggaact caaggcgaat
     7861 cccagatcgt tctgggtgaa ttggacgaag ccgccaggcg gcaggagtgt gccgttcggg
     7921 atgcggaact tgtcgacacc gggcgagtcg ctcaggaagg cgccgcccag attcaccgtg
     7981 cgcgcgccgg cgttgtagag ttcgataaaa tccagcgccg gatagtctgt gtgagccagg
     8041 aactcgttga tcttgatcga cgcccaggcg tcgagtcgga tcggttccat cgcgccgggc
     8101 gaaccgccgc gcatcaggct ggcagaccag gcggcggacc gcccttctcc aaggctgggt
     8161 cgagccagga caagcgagtg ccccgcgccg tccgccgagg ccggccacgg gggctgatcc
     8221 gaatactgga cgtcgacgag gacggcgccc agttcactgt ggagagtgac gcgcccgcca
     8281 ttgccgggca ggttgttctt ataggggccg tagactctcg gaagcccgct cgccgcctcg
     8341 agggccgacg gttctttggc caccaccacg aagccgccgc cgtcgatccg ggtgttcggc
     8401 gggaaggtgt aatcgatcga tcccgacagg cgaaaaccgc caatcgactc ggcaacaggc
     8461 tggctgttga acaactcgac atattcgagg ttcgagccgt ccgccctcgc gagcggattg
     8521 tacatgattt ccgagatgac gagcggcgtg cggcggctcg atgggccgag agactcgacg
     8581 tcggggagcc gctcggcttc cgtcgcgctc acgacgtcgc tgaactcgcg gaaccgggca
     8641 agcagggtgc gattggtctg ctggctgctc accgaaagcc cgagatagac cagcgccggc
     8701 atgggattgg tcacgctccg cagcgttcgc caggcctcgc cgtcgtagct cgaataaccg
     8761 gtaaacagat ttccggttct ccgcaggcgc agccatgtgt acgggtaatt gaccggcatc
     8821 gagggcgaga tcgacagcgg attggtcgct ccgcgcgagg cgaagaagac gccgctgatc
     8881 gatggtgtgg caaagacgcc agccatgggg ccattggtgc tgcgaagggc gcgcgccatc
     8941 aaaccggccg tcgcccatac atccgtcaac tccacggccg ccacccggac ccgcacgtcg
     9001 aaatcgccgg tccgctcctg gtagctgaag tgcaagaggt ccgtccaatc gcccagaccg
     9061 gttcccatcg ccgtgatgtc gaaccctccc ggcacggaga ccgcggcgcc gggcaccgga
     9121 aactcgccca ggtccgacgc cgtgaggatg ctggcaacaa agccaatctg tgaattgggc
     9181 gccaggagat tgcccgccac gctccggtcc ttgagattgt tgacggtcag cagatagggc
     9241 cgcccgaact cgagcgcgtc cacttgaagc gagacgaggc gggcgtcgga ctgcggttgg
     9301 gcgctcagaa cccggccttg atcgagccga taattgccgg caaccgcggc tgacgcataa
     9361 tcgaccggct cggagaaggt caccacaaca gtgcgcgccc cgacattgtg ggcctgcctc
     9421 aggagcggcg gggtgacatc gggctgcacg ctgagcgcgg ccgcggcgct cagggcggac
     9481 cccagcgaat tggtgatggt gcagcggaag ctggcgccgt gatcggataa ccgcacgcta
     9541 ggcagagtgt agctggcgga ggtcgcgccg ggaatggcga ctccgccccg ctgccattgg
     9601 taccggacgg gatcggggtt gttgacgctc acgctgaaga ccgccggctg tccctcgatc
     9661 accgtctggt tggccggctg cgtccggatg acaggcgggg tcagaatagt cccgaaaggc
     9721 accaggcgcg cctcggggat cggctcctcg atcgcgcctg tcgggagctg ccagcgcacg
     9781 gccagattat ccccgccccc cccttccttc atcagggcct cgacgtaata catccgcgag
     9841 gcgtccagcc agatcacacc cgactgctgg ctccggtact tcgtccattc ccgggacgac
     9901 gtccagtcgg gcacggcggc gatgtcgacg gcatcggcgg gcgaggtcgt gctgctcagg
     9961 tagagcacgc tttcgtcgtc gccggcgatc cagaagacat aatcgcccgt cacgggcggg
    10021 acgaggaagc cgcgcaaccg ctggccgtag ttgtccatga cgtccgtcgg cgcctcgaac
    10081 tcgggcagaa tctcggtgag gtccggtcgg tccgggtact tggcgctgtt gaacaagtcg
    10141 tccaagcgcg cgccgggaat gtcggtgtag acctcgcgca acagaccgcc ggcgtgggcg
    10201 gcggtgccga acgacccggt cacaagccac gcaagcagca ggctcgaccg ccccaggccg
    10261 gccatctccc gcgcaatccg gcgcatattc aagggtccca agtgcctcat gttagccccc
    10321 tttctcccgg tccttgtcga tcagcgagcc acagcacggt aaaaacgggc cgcgcggctc
    10381 agggtgtccg gatcgagcca ctggaaggcg gcgctcaccg gccgattggt cgagacggct
    10441 gtccatgcct tgagattggg cgatgcctcg atgacatagg aaacattggg cagacagacc
    10501 accgtcagtt gcacggcgcc gttggccagt cggaccacgt cgatcgacgg acggatattg
    10561 ggcggctcgg gctgctgcaa gggcaggtag acattgtcaa tgaaggcccg gtccaaacct
    10621 tcactgtaat tggcgtcttt cacgtaccgc cactcgagca ctctgcgccc ttcggtgatc
    10681 gggaacatga aggtctgcca gagcacgtcg ccgctccatc gccccagacg ccggccgtcg
    10741 acgtagaatt cgagcgcgtc ccacccttcc tcggagctga cgctcaagtc gaacgccccc
    10801 gtcccgtcgt agaggtagct gatcaggatc agcgagctgg tctggccatt cccgatcgag
    10861 ccgctttgcg ccacgtattg gccctccacc atcgccaccg accatccctt ggccccgctc
    10921 cacgtccagg gcagccgatt cagatcgccg ctgccgaacg tgtcgctgta gttcttcaaa
    10981 cgaaacctcg cgattagact gtagttgcgg tccatcacaa gccgcagcgg gttctgggtc
    11041 gacaccacct ggcccccgat gtcctcccaa tgggcgaaca gatagtcggt ggccgggatc
    11101 gcgttcacga acacttcact gccctccggg taaagcccgc tcgagggcga cacggtcccg
    11161 cccaggggca acgtgaggtc gagactgtag agtgtctcgg tgatgctga
//
LOCUS       DAOQRO010000073        11197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5019_length_11197_cov_4.526746, whole genome shotgun sequence.
ACCESSION   DAOQRO010000073 DAOQRO010000000
VERSION     DAOQRO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_11197_cov_4.526746"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKK20_04225"
     CDS             complement(<1..262)
                     /locus_tag="PKK20_04225"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNU99125.1"
                     /translation="MKRHAQTSIRTTAESDSPPAPLLDKQSERDHRKLRIDKVGVRGL
                     RFPIQVRDKAHTVQNTVATIGMYVDLPQEFKGTHMSRFIEVLN"
     gene            408..1340
                     /locus_tag="PKK20_04230"
     CDS             408..1340
                     /locus_tag="PKK20_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNU99126.1"
                     /translation="MNWLLRCVCGVALAGLAGGHIAAETASTFTVATYNLENYVDVDG
                     GGRVAKPAEARAKIRESIRAMKPDVLAVQEIGTTNTLLELRASLKVEGLDFPHWEYVR
                     GSDTNIAIGLLSRYPIAARRPHPRESFLLLGRRHRVSRGFLEVDIQVTPRYSFTLFNA
                     HLKSRRPIPNADQAELRAQEAILLRKHVDALLEARPKANLVVLGDFNDTKDSVTLQTL
                     LGKRPRALVDTRPAERNGDNAPSPNPRWDARHITWTHFYGTADSYDRVDYILISSAMA
                     REWDRAGSYVLALPNWGVASDHRPVIARFHAEDR"
     gene            1615..1938
                     /locus_tag="PKK20_04235"
     CDS             1615..1938
                     /locus_tag="PKK20_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNU99127.1"
                     /translation="MSSLSAQTSDRLAVLILWGMGVGLALGVILRMIVTWQPGFDTAA
                     GWPAREAIDRLGQVFLRLLFFVVVPLVFASLMLGIIQLGQLSRLVDYAVRLAPMPWLL
                     FPIHS"
     gene            1959..2828
                     /gene="mtnP"
                     /locus_tag="PKK20_04240"
     CDS             1959..2828
                     /gene="mtnP"
                     /locus_tag="PKK20_04240"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNU99128.1"
                     /translation="MTTHRIGIIGGSGLYQIQGFTNQKWMRVRTPFGSPSDELLTGRL
                     AGREVVFLPRHGRGHRIMPSELNHRANIWALKKLGVAWIISVSAVGSLQNRYRPCDIV
                     LVDQFLDRTKRSYEHTFFGRGIVAHIAFAHPICEELRQTLLETARRLKARAHDGGTYV
                     CMEGPAFSTRAESLANRAAGHAVIGMTNLGEAKCAREAQIAYATLAMVTDYDCWKEDG
                     NHVTVDMIIDYLHRNAALAQQIVVQAIPKIPTKPTCSCHSALQNAILTDRSSWPAAAR
                     RQLALLLPGPTTP"
     gene            2825..3277
                     /locus_tag="PKK20_04245"
     CDS             2825..3277
                     /locus_tag="PKK20_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99129.1"
                     /translation="MTGLPRGADPAVAHTRQPILLGRQAKWLADQILTFDLTRQNVID
                     TLRRKPTSQRERAFAVILRNRVQRGGIMAEGAGLVKTGENGRGLNSRWYPETLARRIQ
                     EIAGLRELFSFVEGDAFEVVRRYADEESSRMRAGDSAQKSKPYSSPVP"
     gene            complement(3692..3868)
                     /locus_tag="PKK20_04250"
                     /pseudo
     CDS             complement(3692..3868)
                     /locus_tag="PKK20_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
     gene            complement(3978..5390)
                     /locus_tag="PKK20_04255"
     CDS             complement(3978..5390)
                     /locus_tag="PKK20_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNU99130.1"
                     /translation="MNAKDFLRLGVPLGEATRRATDFVCKFVLGGGDKSRLADEVKAI
                     VENPASFVSDPLRAGFAQALLDAAPPLRAEPISYRQWGSGLESEAVLQMEKACLLPVA
                     VAGALMPDAHVGYGLPIGGVLATVNAVIPYAVGVDIACRMKMTVLDLPLEHLDRRQDR
                     LTRAIEAETRFGVGAAFKERRGHAVMDADWSVSPVTKQHKDKAWAQLGTSGSGNHFVE
                     FGVFTAHGPIRAPGQAQELPAGSYVALLSHSGSRGTGAAVCDYYSKRAFSQFVDMPKE
                     LKRLAWLSLDSQEGLEYWNAMELMGRYAAANHACIHAHIAAHLGAQVLLALENHHNYA
                     WKERHVIGGVEREVIVHRKGATPAGAGVLGIIPGSMASPGFVVCGKGNPESLNSASHG
                     AGRVMSRAQANKTIQWKALKAILRERGVTLISAGLDEAPMVYKNIREVMAAQSDLVTI
                     LGQFDPRLVKMAPPGERAED"
     gene            5697..7700
                     /locus_tag="PKK20_04260"
     CDS             5697..7700
                     /locus_tag="PKK20_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99131.1"
                     /translation="MKKAPSHSIAPTPPSGREDRIEPSSRGANALEGRRRWFRLGAAL
                     LVPLVGIALIELVLRLIGFGYETAFFVRHRAVDPSLRVENQRFAWRFVPRTLAKTPEF
                     VVLNPSKPDGTCRIFVFGESAAIGDPAPAFGFSRILETLLRARYPDMQFEVVNTAFTA
                     INSHVILPIARDCRSLQGDIWLVYMGNNEVIGPFGTGTVFGRQTPPLPVIRTSLALKR
                     ARSAQWLDQWLQRLSPSSEAALGWGGMSMFMSQQVRRDDPRLETLNRHFQRNLGDLIA
                     VGTDARAQVVVSTMVSRLRDWPPFGSLHRIGLTETDRVEWERLYNAGVAAQTNGAFEA
                     AIRDYESAARLDDTYADLHFQWGACCLALGQTNEAKTHLTLARDLDTLRFRTDSRHNE
                     AIRQTVARHAGRGVRLLDAERHFERESKHGMPGEELLYEHVHFTFEGNYLLARLFAEE
                     VAAILADRAAPRSQSSAAWLASEACAARLGYTDSQRYEIAKLVRRRFDEPIYRHQLGH
                     MERLRQLDDELASLRGHSKPVARRRAMEVCRQASASKPGDWMLHELTARLLVGLDDYS
                     AAEAEWREVARLIPHAARSYNEIGKLLEQQGRNDQARASFARALELNPDSADAHVGLG
                     NLDRAQGARSKAIRHFRQALRLDPTRAETAKDLETLLQSEARP"
     gene            7756..8637
                     /gene="panC"
                     /locus_tag="PKK20_04265"
     CDS             7756..8637
                     /gene="panC"
                     /locus_tag="PKK20_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNU99132.1"
                     /translation="MRIVVALHEMQRLARRWRRQGVRVAFVPTMGALHTGHLSLVQEA
                     RRRVKSSGRVVVSVFVNPTQFAPHEDLAAYPRNLARDRALCRTSGVDVLFVPEDTAMY
                     PQAPVPPSSTCVVEEALTRSMEGSARPTHFRGVTTVLAKLFNLVLPDLAIFGAKDWQQ
                     AAVVRRMVRDLNFPLQIVVAPTVRESDGLAMSSRNQRLAPDERPQATVLWRSIQWARG
                     RVQAARRAVPAAQLQKGVAKLVASEPAARLDYVAFFDPDTLEPTRAVHRTSHMALAVF
                     VGKVRLIDNAPLGVDAP"
     gene            8634..9287
                     /locus_tag="PKK20_04270"
     CDS             8634..9287
                     /locus_tag="PKK20_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNU99133.1"
                     /translation="MNCLVTAGPTCEPLDQVRRLTNASTGRLGVELANYLVDRGHVVS
                     LLLGALASWAGPCRANHIERFTTVADLHQRLQQQARKPVDAVFHAAAVSDFRFGHVFE
                     QPTPGELRLLETAKYPTRSGVLLAELVPTRKLIAELRTLFPGAFLAGWKFEVDGTRHS
                     VIQRCLDQLCGCNTDVCVANGPAYGPGYGIVTPGGEVVHAADAAELYARLERRLHAQ"
     gene            9555..9764
                     /gene="rpmI"
                     /locus_tag="PKK20_04275"
     CDS             9555..9764
                     /gene="rpmI"
                     /locus_tag="PKK20_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNU99134.1"
                     /translation="MRRPKGVRTRKAVAKRFKITATGKVLRPHAGRRHLLASKNAKRR
                     RHLRHSAVVEAVDVARIKKSMPYSH"
     gene            9821..10189
                     /gene="rplT"
                     /locus_tag="PKK20_04280"
     CDS             9821..10189
                     /gene="rplT"
                     /locus_tag="PKK20_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNU99135.1"
                     /translation="MRVTNAPASRKRRKRMLQAAKGFRARRSKLYRYASDAVDHGRQY
                     SYRDRKTKKRNFRALWQIRINAAARAAGLTYSRFMEGLKAAKVELDRKMLAELAATDA
                     PTFAELVRAAKTGLQAKTNG"
     gene            10306..>11197
                     /gene="pheS"
                     /locus_tag="PKK20_04285"
     CDS             10306..>11197
                     /gene="pheS"
                     /locus_tag="PKK20_04285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNU99136.1"
                     /translation="MPGFVDQIEPILETALSELRAARDLAVLDQARTTYLGVHGRFTA
                     LMKQLGSLPKEERPDAGKRINTAKGMLEATLAARRQELELAAALPSEPTDFTLPGRRR
                     PLGRLHPLTQVTEDIVRSFRKLGFAVADGPEVEDEHHCFDALNTPADHPARDTQDTFY
                     ISATGAAGVEGEDGTRPAPKWLLRTHTSSVQIRVMAKQPPPVRIIVPGRVYRRDNADA
                     THNPTFHQVEGLYVDRGVAVSDLKGTVEFVFRELMGPETRIRFRPHYFSYTEPSFEID
                     FSSPLTRKMGKEWLEIAGCGM"
BASE COUNT         2058 a         3761 c         3365 g         2013 t
ORIGIN      
        1 cgttgaggac ctcgatgaac cggctcatgt gggtcccctt gaactcttgg ggcaagtcca
       61 cgtacatccc gatcgtggca acggtgttct gcacggtgtg cgccttgtcg cgcacctgga
      121 tcggaaaacg caacccccgc acacccacct tgtcgatccg caacttgcga tggtcccgct
      181 cgctctgctt gtcgagcagt ggagcgggcg gagaatcgct ttccgccgtt gttcggatgc
      241 tggtctgagc atgccttttc atcaatgcgc cggaacgtag cagatggcgc ccctcctgcc
      301 aaaggcattc tggcccggat cagattcagc gcgcagcctg tgatcgatcc gggccaggcg
      361 tattccgttt ttgacgtcgg gacgcaatcc ggcgaggatg gggtcccatg aactggctgc
      421 tgcgttgcgt ctgtggcgtc gccctggccg gcctggccgg cgggcacatc gccgccgaaa
      481 cggcgtccac cttcacggtc gccacctaca atctcgagaa ctacgtggac gtggacggcg
      541 ggggtcgcgt ggccaagccg gcggaagcca gggcgaaaat ccgcgagagc atccgcgcca
      601 tgaaacccga cgtgctggcc gtgcaggaaa tcggcacgac caacaccctg ctggagctgc
      661 gcgcttcact caaggtcgag ggcctcgatt tccctcattg ggaatatgtc cgcggatcgg
      721 ataccaacat cgccatcggg ctgttgagcc gctacccaat cgcagcccgc cgcccgcacc
      781 cgcgagagag tttcctgctc ctgggccgac gccaccgggt cagccggggg tttctcgaag
      841 tcgatatcca ggtcaccccg cgctacagct tcacactctt caacgcccat ttgaaatcgc
      901 gtcgcccgat cccgaacgcg gaccaggcgg aactgcgcgc ccaggaggcc atcctgctgc
      961 gcaagcacgt ggatgcgctg ctcgaggcgc gcccgaaggc gaacctggtg gtgctcggag
     1021 acttcaacga cacgaaggac tccgtgacgc tccagacgct cctgggcaaa cggccgcgcg
     1081 cgttggtgga cacgcgcccc gcggaacgca acggcgacaa tgctcccagc cccaacccgc
     1141 ggtgggatgc gcgacacatc acctggaccc atttctacgg aacggccgac agctacgacc
     1201 gcgtcgatta catcctgatc agctccgcga tggcgcgcga gtgggatcgg gcgggctcct
     1261 atgtgctcgc acttcccaac tggggcgtcg cctccgacca ccggcctgtc atcgcacgct
     1321 tccacgccga agatcgctag cctgcatttc ccaccacttt ttgccaccgc gaagccggag
     1381 ttctcgaaag aagtcagcat gcaggttaga aaaacaaaac cgtgcccgag tggagggtgg
     1441 gaaccgggca acagagtgtc gccgtacccg tgcggtttcg gaggcgttcc gaacctcatt
     1501 ctcccctatc cgggcgcggt tttgcctcaa cccggatttc agattgctcc ttcaagtggt
     1561 gaagaatccg ggctagcgag gcgcgcctcc gattaacccc gccccagttc ccacatgtcc
     1621 tcgctatccg cccagacaag cgaccgactg gcggtcctca ttctgtgggg catgggcgtc
     1681 gggctggcgc tgggcgtcat cctgcgcatg atcgtgacgt ggcaacccgg ttttgacacg
     1741 gcggcgggct ggccggcacg cgaggcaatc gatcggctcg gccaagtgtt tctacgcctg
     1801 ctcttcttcg tcgtggtccc gctcgtcttc gcctcgctca tgttgggcat catccagttg
     1861 ggacagctga gtcggctcgt cgattatgcc gtgcggcttg ccccaatgcc gtggctcctt
     1921 ttcccaatcc actcctgaga aactgacccc tccatcctat gacaacacat cgcatcggca
     1981 tcatcggcgg cagcggcctc taccaaatcc aaggattcac caaccagaaa tggatgcgcg
     2041 tccgaacccc ctttggcagc ccgtcagacg aactgctcac cggacggctc gctggacgcg
     2101 aggtcgtgtt cctgccgcgg cacggacgcg gccatcgcat catgcccagc gagttgaacc
     2161 accgggccaa tatctgggcc ctcaagaaac tgggcgtcgc gtggatcatc agcgtcagcg
     2221 ccgtgggatc gctccaaaac cgctatcgcc cctgcgacat tgtcctggtg gaccaattcc
     2281 tggaccgcac caagcgctcg tatgaacaca ccttcttcgg gcgcggcatt gtggcgcaca
     2341 tcgccttcgc ccatccgatc tgcgaggagc tgcgtcagac ccttctcgaa accgcccgcc
     2401 gcctgaaggc gagagcgcat gacggcggga cttatgtttg catggaaggg ccggcgttca
     2461 gcacccgcgc cgagtcgctc gccaaccgcg ccgcgggcca cgccgtcatc ggcatgacca
     2521 acctcggcga ggccaagtgc gcccgcgaag ctcagatcgc ctacgccacc ctcgccatgg
     2581 tcaccgatta cgactgctgg aaggaggacg gcaaccacgt gaccgtcgac atgatcatcg
     2641 actacctcca ccgcaatgcc gccctggccc aacaaatcgt cgtccaggcc atccccaaaa
     2701 tccccaccaa accaacctgt tcgtgccact ccgcgctcca gaacgcgatc ctcaccgacc
     2761 gatcctcctg gcccgccgcc gccagacgcc aactcgccct cctgttgccc ggcccaacaa
     2821 ccccatgacc ggactccctc ggggggccga tcccgcagtc gcgcatacgc gtcaacctat
     2881 tcttcttggc cgccaggcaa aatggctcgc tgatcaaatc ctcacctttg acctcacccg
     2941 ccagaacgtc atcgatacgc ttcggcgcaa gcccaccagt cagcgggaac gtgccttcgc
     3001 tgtcatcctc aggaaccggg ttcaaagagg ggggatcatg gccgaaggcg cggggttggt
     3061 gaagaccggc gagaacggtc ggggattgaa ctcccgctgg tacccggaga cgctcgcgcg
     3121 acgcatccag gaaatcgccg gcctgcgcga actgttctcc tttgtcgaag gcgacgcgtt
     3181 cgaggtggtt cgccgctacg cggacgaaga aagctcccga atgcgtgcgg gagatagcgc
     3241 gcagaagtcg aaaccataca gttcacccgt gccgtaacgc gaaggcatgc ttccagtcta
     3301 agcacgcaac cgacaaaact gaaagcccgc ttttctgcag ccacgggcga tgtgacggtt
     3361 ttcggcaggt ctggggaaga acgcggcgaa ggttcgcttc gtgaaaggca tgaagcgaaa
     3421 gctgtgcgag gcaacgcgct ggtgaagaat tgtcgttgat caagaaactc ctacttccgg
     3481 gacaagcttc ttgtaaaggg tcgaggatga gtgccaagca ctcgaaagcg tcagcggtcc
     3541 agcgccgagg tgtgttcgct cagccagatc atgttccggt tgttggggga ttcgacgccg
     3601 agggtaagcc agttgttgta tttggctggg gcctttgtgc gagcgtccaa ccacttcctg
     3661 atctcagcat ggccgtcggc atagagccgt gggcgagttg gagctgcttg aggttgctca
     3721 tgcactgaat gccctgcgcc ttggtcttcg ccttgccgag cgccggcagc agcatcccgg
     3781 ccaggatggc gatgatggcg atgaccacca acagttcgat cagcgtgaag gcagcccggg
     3841 cggcgggccc ccgtgccgca aacccaatgg gcaacgtccc aacgggcagg caacgagggg
     3901 tcccgcaaga ggcgaggcag gcccccgcgg atcgaattcg tcccgtgtct cccccattac
     3961 tgaccgttta cgcctactca atcctccgcg cgttcgcccg gcggcgccat cttcaccagc
     4021 ctcggatcga actgcccgag aatcgtcacc agatcgctct gggcagccat gacctcgcgg
     4081 atgttcttgt acaccatcgg cgcttcgtcc aatcccgccg aaatcaaagt cacaccccgc
     4141 tcccgcagga tcgccttgag agccttccac tggattgtct tgttcgcctg tgcgcgactc
     4201 atcacccgcc cggcgccatg ggaggcagag ttcagcgact ccggattgcc tttgccgcac
     4261 acgacgaaac ccggagaggc catcgatcct ggaatgatcc ccagcacgcc tgcgcccgct
     4321 ggcgtggcgc ccttgcgatg aacgatcacc tcgcgctcga cgcccccgat cacgtgccgt
     4381 tccttccacg cgtagttgtg gtgattctcg agagcgagca ggacttgcgc gccgagatgc
     4441 gccgcgatgt gggcgtggat gcacgcatgg ttcgctgccg cgtaacgccc catcaactcc
     4501 atcgcgttcc aatattccaa gccttcctgc gaatcgagag acaaccatgc aagccgcttc
     4561 agttccttgg gcatatccac aaactggctg aaggcccgct tgctgtaata gtcgcacacc
     4621 gccgcccccg tgccccggct tccgctgtgc gagagcaacg ccacgtagct cccggcaggc
     4681 agttcctgag cctgacccgg cgcccgaatg ggcccgtgcg ccgtgaacac gccaaactcg
     4741 acgaagtgat tcccgctgcc gctggtgccc agttgcgccc aggctttgtc cttgtgctgt
     4801 ttggtgaccg ggctcaccga ccaatccgcg tccatcaccg cgtgcccacg cctctctttg
     4861 aaagcggcgc cgacgccaaa ccgcgtctcg gcctcgatgg ccctcgtgag ccggtcctgc
     4921 ctccgatcaa ggtgctcgag cggcagatcc agcacggtca tcttcatccg gcaggcaatg
     4981 tccaccccga cagcataggg aataaccgcg ttcaccgtag ccagaacgcc cccaatcggc
     5041 agcccgtagc cgacgtgcgc gtcgggcatc aatgccccag ccaccgccac cggtaacaaa
     5101 cacgcctttt ccatctgcag gacggcctcc gattccagcc ctgaccccca ttgccggtaa
     5161 gagatcggtt cggcacgcag gggaggggcg gcgtcgagca gcgcctgggc aaatcccgcc
     5221 cgcagcggat cgctcacaaa cgaggccgga ttctcaacaa tcgccttcac ttcgtccgcc
     5281 agtcgcgatt tgtcccctcc cccaaggacg aacttgcaga cgaagtccgt cgcgcgccgg
     5341 gtggcctcac ccaggggcac gcccagccga agaaaatcct tggcgttcat gtcaattgct
     5401 ccatccatag ggtctgtccc aggcttccgg gtccaaagcc ggctgcaagc cgacgctccg
     5461 gacatgggcg gaagggggtc ggatccgaat tcgcgcgatc cgcgccacgc cagaagcgtt
     5521 acgccggctg caagccgacg ctccgaagcc ggacacactc cgtatggcga accgcggcgt
     5581 ttttcgttcg cgcgctgcgg gccggcaggc tagaatcctc gaccggtgaa acgctttgta
     5641 cacaagtcaa gtctctgtgc tgcgttgacg gggtcaagga ttctcttggg atagcggtga
     5701 agaaggcccc atctcattcg atagcgccga cgcccccatc cgggcgggag gaccggatcg
     5761 agccgtcgtc tcggggcgcg aacgcgctcg agggccgacg gcgctggttt cgccttggcg
     5821 cggcgctgtt ggtgcccctg gtgggaatcg ctctcatcga gttggttctg cgcctgattg
     5881 gctttggata cgagacggct ttcttcgtgc gccatcgcgc cgtcgacccg agtctgcggg
     5941 tcgagaacca gcgattcgcc tggcgatttg ttccgcgcac cctcgccaag acccccgagt
     6001 ttgttgtgct caatccatcc aaaccggatg gaacctgccg gattttcgta ttcggcgaat
     6061 cggctgcgat cggagatccg gcgccggcgt tcggtttctc gcgcatcctc gagaccctgc
     6121 tgcgcgcgcg ctacccggac atgcaattcg aagtcgtcaa caccgcgttc acagccatca
     6181 attcgcatgt gatcctgccg attgcgcggg actgtcgaag cctgcaaggc gacatctggc
     6241 tggtgtacat ggggaacaac gaggtcatcg gccccttcgg aaccggcacc gtctttggcc
     6301 gccaaacgcc cccgcttccc gtcatccgga cgagtctcgc gctcaaaagg gcccgctccg
     6361 cccagtggct cgaccagtgg cttcaacgat tgagcccctc atccgaagcg gccctgggct
     6421 ggggcgggat gtcgatgttc atgagccaac aggtgcgccg cgacgatccg cgtctcgaga
     6481 ctctgaatcg ccatttccaa cggaatctgg gcgacctcat cgcggtgggc acggacgcgc
     6541 gggcgcaggt ggttgtgagc acaatggtgt cgcgcctgcg cgactggccg cccttcgggt
     6601 cgctgcatcg gatcggcctg actgagaccg atcgggtcga gtgggaacgc ttgtacaacg
     6661 ccggcgtcgc agctcaaacc aacggtgcat tcgaagccgc aattcgggac tacgaatccg
     6721 ccgcaagact cgatgatacc tacgccgatc tgcacttcca atggggcgcc tgttgcctgg
     6781 ccctcggcca gaccaacgag gccaaaaccc atctgaccct cgcccgagac ctcgatacgc
     6841 tccggttccg gaccgactcc cgccacaacg aggcgatccg ccagactgtt gcgcgccacg
     6901 ccggacgcgg cgttcgtctc ctggacgcgg aaaggcactt cgagcgcgag agtaagcacg
     6961 gcatgcccgg cgaggaactg ctctatgaac acgtccactt cactttcgag ggcaattacc
     7021 tcctcgctcg gctcttcgcc gaggaggtcg ccgccatcct cgcggatcgc gccgccccgc
     7081 ggtcgcaatc gtcggctgcc tggctcgcat ccgaggcttg cgctgcgcgt ctgggctaca
     7141 cggacagcca acggtacgag atcgccaagc ttgtgcggcg ccgttttgac gaaccgatct
     7201 accggcatca actcggccat atggaacgtc tgagacaact cgatgacgaa ttggccagcc
     7261 tgcgcggcca ctccaaacca gtcgcccgcc gccgcgccat ggaggtctgc cgacaggcct
     7321 cggcgtccaa gcccggcgac tggatgctgc acgaactgac cgcccgcctg ctggttggac
     7381 tcgacgacta cagcgccgcc gaagcggaat ggcgcgaggt cgcccgcctg attccccacg
     7441 ccgcacgctc gtacaacgaa atcggcaaac tcctcgaaca gcagggaagg aacgatcaag
     7501 cgcgcgcctc ctttgccagg gccctggaac tgaatccgga ctccgctgat gcccacgtcg
     7561 ggctgggcaa tctcgatcgg gcccagggcg ctcgatcgaa ggccatccgc catttccgcc
     7621 aagccctgcg cctggacccg acccgcgcgg agaccgcgaa agacctcgaa acgctcctgc
     7681 aatccgaagc caggccttga cggcaacgca ggatttgagt cgcacaccac cctcggacgc
     7741 ttcatcatca tcgccatgcg aatcgtcgtc gccctgcacg agatgcaacg tttggctcgc
     7801 cgttggaggc gtcaaggggt gcgcgtcgca ttcgtgccca cgatgggcgc cctgcacacg
     7861 ggacacctga gccttgtcca ggaagcgcgc cgacgtgtga aatcaagcgg gcgcgtcgtt
     7921 gtcagtgtgt tcgtcaatcc cacacagttc gcgcctcatg aggacctggc ggcgtatccg
     7981 cggaatctgg cgcgggatcg cgccctctgc cggacgtccg gggtagatgt tctcttcgtg
     8041 cctgaggaca ccgcgatgta cccgcaggcg cccgtcccgc cttcgagcac ctgcgttgtc
     8101 gaggaagcgt tgacccgctc gatggagggg tctgcgcgac cgacccattt ccggggcgtc
     8161 acgacggtcc tggccaaact gttcaacctg gtgttgcccg acctcgcgat cttcggcgcg
     8221 aaagactggc agcaggcggc ggtggtgcgc cgcatggtgc gggacctcaa cttcccgctg
     8281 cagatcgtgg tggcccccac ggtgcgcgag agcgacggac tggccatgag ttcgaggaac
     8341 cagcgcctgg cacccgacga gcgccctcag gcaaccgtgc tttggcgctc gatccagtgg
     8401 gcccgaggcc gcgtccaggc cgcccggcgt gccgtccccg ccgcccaact gcagaaaggg
     8461 gttgccaagc tggttgcgtc tgaaccggcc gcccgcctcg attatgtggc ctttttcgat
     8521 cccgacaccc tcgagccaac tcgtgcggtg catcggactt cccacatggc cctggccgtg
     8581 ttcgtaggca aggtccgact catcgacaac gccccgctcg gagtcgacgc accgtgaact
     8641 gcctggtcac cgccggtccg acttgcgaac ctctcgatca ggtccgacgc ctgacgaatg
     8701 cgtcgaccgg gcgattgggg gtcgaacttg ccaattacct ggtcgacagg gggcacgtgg
     8761 tgtccctgct gctcggcgcg ctcgcctcct gggcgggccc ctgtcgggca aaccacatcg
     8821 agcggttcac gacggtcgcc gacctccatc agcgcctgca acaacaggcc agaaagcccg
     8881 tcgacgccgt gttccatgcc gcggcggtga gcgatttcag gttcggacac gtcttcgaac
     8941 aaccgacgcc gggcgagttg cgtcttctcg agaccgccaa gtacccgacc cgctcaggcg
     9001 ttctgctcgc tgagcttgtt ccgacgcgga agctgatcgc cgaactgcgg accttgtttc
     9061 ccggcgcgtt cctggccgga tggaaattcg aggtggacgg gacgcgccat agcgtgattc
     9121 aacggtgtct ggaccaattg tgcgggtgta acaccgacgt gtgcgtcgca aacgggccgg
     9181 cttacggccc cggctatggg atcgtcactc ccggtggcga ggtcgtccac gcagcggacg
     9241 cagccgagct gtacgcgcga ctcgagaggc gattgcacgc ccagtgacaa acaggccttg
     9301 cccagcgcgc atgagtgcac cttccaatgc cgtcggctcc gcgttcttca gaagtgggtg
     9361 agaaatgcgc gctaccgtgc cccgtgtcaa tctcttctcc ggttcctcct ttccaacacc
     9421 gtcaaccgtg cactgcgcag attccttgtc gcgatggcga ctcgagattt gggtttggtt
     9481 tttggcattt caggcttgca ctgcgttttg gggctgccta gattgcgagc tctttttcgc
     9541 taggaatacg atttatgcga cgccccaaag gtgtaaggac ccggaaggca gtggccaagc
     9601 ggttcaagat caccgccacc ggaaaagtgt tgcgacccca tgcgggccgc cgccatttgc
     9661 ttgcttcgaa gaacgcgaag cgccgtcggc atctgcgtca ctcggcggtg gttgaggccg
     9721 tcgacgtggc aaggatcaag aagagcatgc cgtacagcca ctaggatgcg gtcgacaatg
     9781 ccgcggactc tcctgtttta aaccgttaac taaagttctc atgcgcgtta ccaacgcccc
     9841 agcctctcgt aagcgccgca aacgcatgct gcaagccgcc aaaggcttcc gtgcccggcg
     9901 ttccaagctc tatcgctatg cttccgacgc cgtcgatcac gggcggcagt actcgtatcg
     9961 cgaccgcaag accaagaagc ggaacttccg ggcgctgtgg cagattcgga tcaacgctgc
    10021 ggcccgcgcc gcaggcctga cctacagccg gttcatggaa gggctcaagg ccgcgaaggt
    10081 cgagctggac cggaagatgc ttgccgagtt ggcggcaacg gatgccccaa cgttcgccga
    10141 gttggtcagg gcggccaaga ccggcctgca agccaagacg aacggctgat cgaggatcgg
    10201 cgactcgccc ctgagccata gtttcgctcg cgaactcatc tgtcgaatgg ctggcgtttg
    10261 ctgatccctt cttccgagcg ggtccccacg cacccacccc aacgcatgcc aggcttcgtc
    10321 gaccaaatcg agccgatcct cgagacggca ctgtcggagt tgcgcgcggc gcgggatttg
    10381 gccgtcctgg atcaggcgcg cacgacctac ctcggcgtgc acgggcgctt caccgcgctg
    10441 atgaagcaac tgggatcgct gccgaaagag gagcgcccgg acgccggcaa gcgaatcaac
    10501 accgccaaag gcatgctcga ggcgacgctg gcggcacgca gacaggaact cgaattggcg
    10561 gccgccttgc cgagcgagcc gaccgatttt acgctgcccg gacgccgccg tccgctgggc
    10621 cggttgcacc cgctcacgca ggtcactgag gatatcgttc gaagctttcg caagctcggt
    10681 tttgccgtgg ccgacgggcc ggaggtggaa gacgagcacc actgcttcga cgcgttgaat
    10741 acgccggcag accacccggc acgggacacc caggatacgt tctacatctc cgccacaggc
    10801 gcggcgggag ttgagggcga ggatggaacg cgaccggcgc cgaagtggct gctgcgcacg
    10861 cacacctcct cagtccaaat ccgcgtgatg gccaaacaac cgccgccggt gcgcatcatc
    10921 gtgccggggc gggtctatcg acgcgacaat gcggatgcca cccacaatcc gacctttcac
    10981 caggtcgagg gattgtacgt ggatcgggga gtggccgtct ccgatctgaa ggggacggtc
    11041 gagttcgtgt ttcgggagct gatgggtccg gaaacgcgca ttcgcttccg cccacactac
    11101 ttcagctata cggagccgag tttcgaaatc gatttttcga gtccgctcac gcggaagatg
    11161 ggcaaggaat ggctcgagat cgccggatgc ggcatgg
//
LOCUS       DAOQRO010000074        11183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5027_length_11183_cov_5.026959, whole genome shotgun sequence.
ACCESSION   DAOQRO010000074 DAOQRO010000000
VERSION     DAOQRO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5027_length_11183_cov_5.026959"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..2162
                     /locus_tag="PKK20_04290"
     CDS             201..2162
                     /locus_tag="PKK20_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99137.1"
                     /translation="MKISTDRSLVARCRAGKESAWDQLLESHYDGIGRFVFLAAPGVT
                     ESEVPEICRRTFIRAVGEINEVMDAPSCRVWLLRVASEEVRRIGRERLESFGQGPCDQ
                     CSSVAASELSDGRGVDGNALMRQLRGQFDRFGGPCIELAELWFFSELPEAEVAAAFDV
                     SPDLLLRRLRMCLQRMQTLEPLRKSDVQESLDMPYPPDFARRDVGHPLGTRLAVYAKE
                     RGALITHRFPIPQAIREDVLAAVRSAFRKPCAVLPRSVMSLLVPHWRLTATVAGGALV
                     VVIALLHGQGGGAPAGTRRTGVSNCYWSIKSELPRVLLPNSEPDLTCPTGSDGELQWS
                     IAPAQQAKQEAAPPNATDQIQSEDPAGSLVMGSDQPEPIMDAVPATEDVTDSARAEVV
                     ESGVAESSPRVSDVAETREDLTAASEPRTVQPEPILALASSAPPADSSAPDVGTQPPP
                     PSVLIAMVDRPTSPTRASARTDIGIEAGLTSTSEVTTRQRQFIRANMASQFRRNFNSP
                     PMPAVLESFDLDLTQSGLTITDADGSEYRGTVDWAAAQHKDDATRQARSLAFEVKGVH
                     RTTGEFVVFTGWLVPQRAGSILLSPMTSSPSDPAATSRMPLLAEGVLAIEGNVQYGAR
                     TRFPVHAGAARHVTPEEVAGPATHRVPST"
     gene            complement(2110..2883)
                     /locus_tag="PKK20_04295"
     CDS             complement(2110..2883)
                     /locus_tag="PKK20_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNU99138.1"
                     /translation="MQPEERQHRIEEYLQRVEFASLEELAREVGASLSTVRRDLAVLE
                     GGGGIRRTHGGARILNPRTDEFAFSARDTHQLAEKEAIGRLCAELILPGQSVIIDAGT
                     TAYHAARYLESKRPLIITNSLPVANHYASNNMVEIVVTGGVVYPRLGVLVGPLAIESL
                     TRLHADVAIMGAGGITSDGISNSHVLLIEIQRAMMRAAQTVVFCMDHTKFGRKSMVHL
                     CGFERVRAVVADTNAPADLVQELRSKGLDVLQAPLPLQA"
     gene            2969..3646
                     /locus_tag="PKK20_04300"
     CDS             2969..3646
                     /locus_tag="PKK20_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HNU99139.1"
                     /translation="MTPTVPVTHRAVVEHLVRQMVYQKLGKPLPKVALAPNPLVVNVS
                     ARHCHLTPEAVEALFGKGAQLRVHKELYQPGAFAAKETVTLIGPRSRVISNLRILGPC
                     RNLNQVELAYTDAIALGFDIPLRISGDIKGTPGCMLMGPAGFFEMEQGVIRALRHVHM
                     HPDDAAFYGVKSGDEIRLKIGGPCGIIFDRLLCRVDASFKLEAHIDTDEGNACNLLPG
                     TPCELVN"
     gene            3725..4018
                     /locus_tag="PKK20_04305"
     CDS             3725..4018
                     /locus_tag="PKK20_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNU99140.1"
                     /translation="MSEALGMIETKGYVGAVEASDAMVKAANVTLVKTIPIGGGMITV
                     LARGDVGSVKAAVDAGSKAASKVGELISSHIIARPHEDLLRAFVGDAAKSGGK"
     gene            4063..4341
                     /locus_tag="PKK20_04310"
     CDS             4063..4341
                     /locus_tag="PKK20_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNU99141.1"
                     /translation="MVQQAIGMIECKGLCALLEASDAALKAANVVLLGWEKIGSGYVT
                     SIFRGDVAAVKAATDAGAAAAAQVGQVVSVQVIPRPHEGLSALGKWLQ"
     gene            4398..5567
                     /locus_tag="PKK20_04315"
                     /pseudo
     CDS             4398..5567
                     /locus_tag="PKK20_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
     gene            5682..5963
                     /locus_tag="PKK20_04320"
     CDS             5682..5963
                     /locus_tag="PKK20_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNU99142.1"
                     /translation="MPQAIGLLETRGLVALVEGTDAMLKAANVELAGPMTQVGNALVT
                     AVVVGDVAAVKAAVDAGAQAVKSIQGELISVHVIARPHPDVDAVMPKRK"
     gene            6074..6406
                     /locus_tag="PKK20_04325"
     CDS             6074..6406
                     /locus_tag="PKK20_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNU99143.1"
                     /translation="MYLAHVEGSVVATKKDAHMNGRKLLMLRPMLVDDKDPTRFRPGV
                     NTIVAVDSVGAGEGELVLFCQGSSARLAPGLKDCPVDAVVIGIVDSVDVLGRQIYTAR
                     SGATAKPA"
     gene            6517..8019
                     /locus_tag="PKK20_04330"
     CDS             6517..8019
                     /locus_tag="PKK20_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HNU99144.1"
                     /translation="MSSQANEPLIRDIVQEVLGRLGRSAPSGAGKAGTPAAAPSPACD
                     RPGPTHAPASRPGRYGVFGDANEACAAAHAAYRQLSEKGVAGRNTVVRIIKEICCAKS
                     KEWGRLELDETKIGRLDHKIEKLEGLRAVPGVEFLQPYGLSGDHGITLEECAPFGVIG
                     AVTPSTHSIPTVACNIISMVAAGNSVVFNAHPGAAKCALAAVRVFNESIHRELGIENL
                     ACLIDPPTLDSFQALAKNEHVKLLCVTGGPGVVKAAMASGKRAVCAGPGNPPVLVDGT
                     SCLERAARQVILGAAYDNNVLCIGEKEVFVLDRVFDAFLAALEKNGACRLSGAQVERL
                     TQAAFTFLPGHGGGCAEPVVNRALIGKDVPVLAQAAGINAPAGAPLLFAETEANHPFV
                     QEEQMMPFLPVVRVRSVEEGIEGARIAERGYKHSAMIHSHDVANMTAMAKALETTLFV
                     KNGSCLAGLGNGGEGYPNFSIATTTGEGICNPRTFTRMRRCVMVDKLRIF"
     gene            8033..8299
                     /locus_tag="PKK20_04335"
     CDS             8033..8299
                     /locus_tag="PKK20_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNU99145.1"
                     /translation="MLLARVEGSLIATRKHASFDGWRMVICQPINAAGGAEGPPQVAI
                     DPHGAGLHQRVIISSDGAATRSAVGDPRSPARWLVIGVVDSEAG"
     gene            8301..8603
                     /locus_tag="PKK20_04340"
     CDS             8301..8603
                     /locus_tag="PKK20_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNU99146.1"
                     /translation="MRVGSVIGRVTLSQTIPAFCGARWLLISPFTRDHFQHRHNPPTG
                     LSKDFSLVAYDDLGGGLGDTVGYVEGREAAMPFAQPTPVDAITVAIVDDVFYTPLA"
     gene            8600..9667
                     /locus_tag="PKK20_04345"
     CDS             8600..9667
                     /locus_tag="PKK20_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNU99147.1"
                     /translation="MKKVYSLRDVEEMIRRGEDLKAIPGDAILTPSARDLLREQIDHP
                     RPATPLRSAQAPLPTDVRPSPPARPQTSKSPRAELDAFFNSPYCHDLKQQMCEVGHRL
                     WQREYVDGNGGNIAIRVGEDLALCTPTLVSKGSLKPEDMCLVDFEGAQLCGTKKRTSE
                     ILMHLQMMKRQPLDVASVHCHPPYATAFAVAGVEPPTCMIPEYEVFCAVAIAPYRTPG
                     SPEMGRLVADLVDKHNTILMANHGVVAWSHNSVEEAYWRIEIIEAYCRTLTVAVQLGK
                     PLNTFTPGQLQDLLKIKQNLGFVDPRYGLKECELCNNDEWRPGVTCQIPARETMLGLD
                     PEAEELVQAVTDQIVARLKND"
     gene            9765..11042
                     /locus_tag="PKK20_04350"
     CDS             9765..11042
                     /locus_tag="PKK20_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99148.1"
                     /translation="MKSLDAKLAEIKANPASRAFILADAKDADMAFGVRAMGPRHYLS
                     SHGAQAARFSPEVWTREEFGHRNLPEFLDIIREVVGQGLVDIMLMSASVNEQLAIKEG
                     LFRKSHVTPAARANDATDVWAVRHGSYLHEPSHPFRSATIDHIQCGQAQCDRSADDVP
                     GANLGLYSITFVNNLEQDLASLQAFKEFRIEAERKKFRYFLEVFDPNVANGIPPEKLG
                     EFINDNIVRSLAGVTSAGRPLFLKIAYHGPRLMEELAQYDPNLIVGVLGGSAGTTHDA
                     FRLIHDAQKYGARVALFGRKINNAEHQLAFIEMLRLITDGRIAPDEAVRAYHNILGKL
                     GLQPKRPLEKDLLLTDQAMSYGGSPTRVSVPVAANPLAPAIAPGAAPKPPAGPAANAP
                     AWPVRPDGSPDFATMTSAERRAYDADRLKRRFG"
BASE COUNT         2044 a         3500 c         3620 g         2019 t
ORIGIN      
        1 cggtcgaatc ttgcggattg aggcaacacc cgagtccccc tgacgtgtgc tgcatgtgaa
       61 taattcactc atagtccctg ttcagcgcgt cttgcatgaa cttgcttaat tcatgactgc
      121 gtttttctga aagttcttgt tgattgctgg agaatatctc tgcatactcg acgtaagcaa
      181 tcgtcgttac ttagcatgca gtgaagatct ctactgacag gagtctggtc gcccgctgtc
      241 gtgcggggaa agaatccgcc tgggatcagt tgctggaatc tcactatgac ggcattgggc
      301 gttttgtgtt tcttgccgcc ccgggtgtca cggaatccga ggtcccggaa atctgccgcc
      361 ggacgtttat tcgtgcggtt ggcgagatca acgaggtcat ggacgcgccc tcctgccgtg
      421 tttggctgct gcgtgtggcg tcggaagagg tgcggcggat cggacgtgaa agactggaga
      481 gcttcgggca agggccatgc gatcagtgct cgagcgtggc ggcgtcggaa ctcagtgatg
      541 gccgcggcgt tgacggcaat gcgctgatgc ggcagctccg cggtcaattc gaccggtttg
      601 gcgggccatg tatcgagttg gccgagttgt ggtttttcag cgagcttccg gaggccgagg
      661 tggctgcggc gttcgatgtg agtccggacc tgctgttgcg gcggttgcgg atgtgtttgc
      721 agcgaatgca gacgctcgaa ccgctccgga aaagtgatgt gcaggagtct ctggatatgc
      781 cctatccgcc cgactttgca cgccgggatg tcgggcatcc actgggcaca cgcctcgccg
      841 tctatgcgaa ggagcggggc gccctcatca cccaccgatt cccgattccg caggcgatcc
      901 gggaagacgt tctcgcggcg gtgcggtcgg cattcaggaa gccatgcgcg gtgctgcccc
      961 gatctgtgat gtccttgctg gtgccgcatt ggcgtttgac tgccacggtt gccggtggcg
     1021 cgcttgtggt ggtgatagcg cttctgcacg gccagggggg cggggcaccc gccgggacgc
     1081 gccgcacagg cgtttcgaac tgttactggt ccatcaagtc ggagctgcct cgagttcttc
     1141 tgccgaactc cgagcccgac ttgacctgtc ccaccggaag cgatggggag ctgcaatgga
     1201 gcattgcccc tgcacaacag gcaaagcagg aagctgcgcc tccaaacgca acggatcaga
     1261 tccaatccga agacccggca gggagtctcg tcatgggcag cgatcagccc gagccgatca
     1321 tggacgccgt tccggcgacg gaggatgtga cggattccgc gcgcgccgag gttgtggagt
     1381 cgggagttgc tgagtcatcg cctcgggtgt cggacgttgc cgaaacccgt gaagacctca
     1441 cggcggcatc ggagccaagg acagtccagc ccgaaccgat ccttgccctg gcatcctctg
     1501 cgccgccggc ggattcgtca gctcccgacg tgggcacaca gccgcccccg ccatcggtct
     1561 tgatcgcgat ggtcgataga ccgacctcgc caacccgcgc gtccgctcgg acggacatcg
     1621 ggatcgaggc agggttgaca tcgacaagcg aagtgacgac gcgccagcgg cagtttatcc
     1681 gcgcgaacat ggccagccag ttccgtcgca acttcaactc gccgcccatg ccggcggtgc
     1741 tcgagagctt cgacctcgat ttgacgcaat cgggcttgac gattaccgat gccgacggct
     1801 cggaatatcg ggggacggtg gattgggcgg cagctcagca caaggatgac gccacgcggc
     1861 aggctcgcag tctagcgttc gaggtcaaag gcgtgcatcg cacgactgga gaattcgttg
     1921 tgttcacagg ctggttggtt ccccaacgcg caggctcgat cctcctctcg ccgatgactt
     1981 caagtccgtc ggacccggcc gcgacctctc ggatgccgct gctggcggag ggggtcctgg
     2041 cgatcgaggg gaacgtgcaa tacggcgccc ggacgcgttt tccggtgcat gcgggcgctg
     2101 cgcggcacgt cacgcctgaa gaggtagcgg ggcctgcaac acatcgagtc ccttcgacct
     2161 gagttcctgc accagatccg ccggcgcgtt ggtgtccgcc acaacggcgc gcacccgctc
     2221 gaacccgcag agatggacca tagacttgcg cccgaatttg gtgtggtcca tgcagaacac
     2281 caccgtttgc gcggctctca tcatcgcgcg ctggatttcg atgagcagga cgtgggaatt
     2341 cgagatcccg tcggatgtta tcccgcctgc gcccatgatg gccacgtcgg catgcaggcg
     2401 cgtgagggac tcgatagcca ggggaccgac gagaacaccc aggcgcggat agaccacccc
     2461 gcctgtcacc acgatctcga ccatgttgtt cgatgcgtag tgattcgcga ccgggagcga
     2521 gttggtgatg atgagcgggc gtttcgattc aagatagcgg gccgcgtggt aggccgtcgt
     2581 ccccgcgtcg atgatgacgc tctggcccgg cagaatcaac tccgcgcaca gccgtccgat
     2641 ggcctctttc tccgccagtt gatgcgtgtc gcgcgcactg aacgcgaact cgtcggtgcg
     2701 cggattcagg atgcgcgccc cgccgtgggt gcgtcgaatg ccgcccccgc cttcgagcac
     2761 cgccaggtcg cgacggacgg tcgacaggga ggctccgacc tcgcgcgcga gttcttcgag
     2821 agaggcgaat tcgacgcgct gaagatattc ttcaatgcga tgttggcgtt cttcaggctg
     2881 cacgcgccct gcttgtgaga gattttgaaa cattttgcaa gattccagtt gacatctgag
     2941 tgcggacaag aaggatttcg gtcagttgat gacaccaaca gtgccagtta cgcatcgtgc
     3001 ggttgtcgag catctggtgc gtcagatggt ctaccagaag ttgggcaaac cgttgccgaa
     3061 agtcgcgtta gctcccaacc cgcttgttgt gaacgtgagc gcccggcatt gccatttgac
     3121 gcctgaggcg gtcgaggcgt tgtttggcaa gggggcccag ctccgtgtgc ataaggagct
     3181 ctaccagccc ggggccttcg cggcgaagga gaccgtgacg ttgattggcc cgcgcagtcg
     3241 tgttatatcg aacctgcgga ttctagggcc gtgccgcaat ctgaatcagg tcgagttggc
     3301 ctatacggat gcgatcgcgc tgggatttga catcccgctg cggatttcag gggacatcaa
     3361 ggggacgccg ggctgcatgt tgatggggcc agcggggttt ttcgagatgg agcagggtgt
     3421 cattcgcgcg ttgcgccacg tgcacatgca tccggacgac gccgcgtttt atggggtcaa
     3481 gagcggggat gagatacggc tgaagatcgg cggtccgtgc gggattattt tcgacaggct
     3541 tttgtgccgg gtggatgcga gtttcaagct cgaggcgcac attgacacgg acgaggggaa
     3601 tgcgtgcaac ctgttgccgg ggacaccgtg tgagttggtc aactgatgaa gtgaacagcg
     3661 ggatcgcggg cgcgtggcgc cccgggcgag tttccgaacg agaaccacta acgacagact
     3721 gattatgagc gaagcactcg gaatgatcga aaccaaaggc tacgttggcg ccgtcgaggc
     3781 cagcgacgcg atggtcaagg ccgcaaacgt gaccctcgtc aagaccatcc cgatcggggg
     3841 tgggatgatc accgtgctgg cgcgtgggga cgtggggagc gtcaaggcgg cggtggatgc
     3901 gggctcgaag gcggcgagca aagtgggcga attgatcagc tcccacatta tcgcgcgtcc
     3961 gcacgaggat ctccttcggg ccttcgtggg tgatgcggcc aaatccggag gcaagtagaa
     4021 tcctcaagat tgctctaaac caaccatcaa ggaatctctg ttatggtaca acaagcgatt
     4081 ggaatgatcg agtgcaaggg cctctgcgcc ttgctcgagg cgagcgacgc cgccctcaag
     4141 gccgccaacg tagtcctcct gggctgggag aagatcggga gcggttatgt gacatcgatc
     4201 ttccgcggcg acgtggcggc tgtcaaagcg gccaccgacg ccggcgcggc cgcggccgcg
     4261 caagtcggcc aggttgtcag cgtgcaagtg atcccccgac cgcatgaggg tctgagcgcg
     4321 ctcggcaagt ggctccaata agctcgggtg acgcgcaatc ggcagcgcgg gtcgctgccg
     4381 accggacctc ttcccgaatg aagatccttg tcgcgaacat cggctcgacc tcgctcaagt
     4441 ggcggctctt tgactttgcg gatggggagc ggctgcttca caagggcggc ttcgagcaag
     4501 tgggcgatta cgcacgggcc atcgaggatt gtctggcgga tctcgagcgt tccggggtcg
     4561 tgacatccga gcgcgagatc gccgcggtgg gtttcaagac cgttgtggcc aaaggcgtga
     4621 gcggctgtgt gcgcctggat gattcggcgg ttgcggcgat ggaggcgttc aatcagctcg
     4681 ccccggccca caatcccgcg tatgtgcgcg gcatccgcct gttcgcgcag cgcctgccgg
     4741 aggttcccct ggtgggcttg ttcgaaaccg cgttttacca atgggcgccc gaggcggcga
     4801 tgcgctacgc tgtgccggcg tcgtggtgcg atgcgggagt gcgtcgctgg ggcttccatg
     4861 gcgccagcca caagttcatc gccgagcgca gcgccgaact gctcgggcgc gacgatgtgg
     4921 ccgaacgcgc gcggcgtctt tacgtcgacg acggcagcac gccggtgcgc gagcctgcct
     4981 tgcgcgtcgt ttcctgtcat ctgggcggga gctcgagcat cagcggcatc cgcaatggcg
     5041 tggcggttgg caacagtctg gggatgagcc cgcaatcggg tgtgccgcac aacaaccggg
     5101 tgggcgacct ggacgcgatg gcttttccct ttgcgattcg ggcgcttggg ttgactgtcg
     5161 aggaggctga acgccagctc tgcaacgagg gcggaatcaa gggcctgagc ggcgtgtcga
     5221 acgacgtgcg caatgtctgg acggcggcgc aggggggcaa cgcccaggcg cgcctggcgc
     5281 tcgaggtgtt cgtgcacgag gtccgccgat ggatcggcgg ctatttcgcc caactcaacg
     5341 gggcggacgc cctggtattt acagcgggta ttggcgagaa ccgatccgag cttcgggcgg
     5401 ccatctgcgc cgacctggat cagttgggcg tcgttctgga tgcgaacgcg aacgtgaagg
     5461 tgcgcgccca ggaggctgtc atcagcgcgc cggaatcccg cgtgcgcgtg ctggtcatcc
     5521 ccaccaacga ggaactcgtc gtggcccgcg aggtcaagcg ctggctggat cgtcgtggtt
     5581 cgaacaacga agccggtgcc gcagacccat ctttcaactc cagcctgacc cgactctcat
     5641 cccacacagc atccaactct caactctcga aagaactcca catgccacaa gccataggac
     5701 tccttgaaac ccgcggcctg gtcgcccttg tcgagggcac cgatgcaatg ctcaaggccg
     5761 cgaatgttga actggccgga ccgatgacac aggtcggcaa cgccctggtc acagccgtcg
     5821 tggtcggcga cgtcgcggcc gtgaaagccg cggtggacgc cggagcccag gccgtgaaat
     5881 cgatccaggg cgaactgatc agcgtccatg tcatcgcccg tccgcacccg gatgtggacg
     5941 cggtcatgcc gaagaggaaa tgacggtccg atcctcgagg cgggaaccgc cgggcacgag
     6001 gccggaagcg ggtgaccgtt ccagcctcgt ctcgcggctc ggggattgcg catgagactt
     6061 tggcccagcc gttatgtacc ttgctcacgt tgaaggctca gtggtcgcca ccaagaagga
     6121 cgcgcacatg aacggccgca agctgctcat gctgcgtccg atgctggttg acgacaagga
     6181 tccgacccgg ttccgtccgg gtgtgaacac gattgtcgcg gtggatagcg tgggggccgg
     6241 cgagggtgag ctggtgctgt tctgccaggg gagttccgct cgacttgccc ccggactcaa
     6301 ggactgccct gtggacgctg ttgtgatcgg catcgtggac agcgtcgacg tgctgggacg
     6361 ccagatttat accgcgcgaa gcggcgcgac ggcaaagccc gcatgaaagg caacaggcga
     6421 cgcatcgagg cagaatgtgt tcccgggcct gcaccccggt gcggaaccga ggcggcccgc
     6481 gcctgaaaga cccaaacata gaccacgttt tccgttatga gctcgcaagc gaacgaacct
     6541 ctcatccgcg acatcgtcca ggaagtcctg ggacgcctgg ggcgcagcgc cccttcaggt
     6601 gcgggcaagg ccggcacacc ggccgctgcg ccttctccag cgtgcgatcg tcccggcccc
     6661 acccatgctc cggcgtcgcg gcccgggcgt tacggcgtat tcggcgacgc gaatgaggcg
     6721 tgcgcggcgg cgcatgcggc ctatcggcag ctttccgaaa agggggtggc agggcgcaac
     6781 acggtcgtcc gcatcatcaa ggagatctgt tgcgccaagt cgaaggaatg gggccggctc
     6841 gaactcgatg agacgaagat tgggcgcctg gaccacaaga tcgagaagct cgaaggactg
     6901 cgcgccgtgc cgggcgtcga gttcctgcaa ccttacggcc tgagcgggga tcacgggatc
     6961 acgctcgaag agtgcgcccc gttcggagtg atcggcgccg tgacgccgtc cacgcactcg
     7021 attcctacgg tggcgtgcaa catcatcagc atggtggccg cgggcaactc cgtcgttttc
     7081 aacgctcatc ccggagccgc gaagtgcgcc ctggcggcgg tgcgagtatt caacgagtcc
     7141 atccaccgcg agttgggtat cgagaacctg gcgtgcctga tcgacccgcc cacactcgat
     7201 tccttccagg cgctcgccaa aaacgagcac gtcaagctcc tctgtgtcac cggcggccca
     7261 ggcgtggtta aggcggccat ggccagcggc aagcgcgcag tctgcgccgg gcccggcaac
     7321 ccgcccgtcc tggtggacgg cacgtcctgc ctcgaacgag ccgcccggca agtgatcctc
     7381 ggagcggcct acgataacaa cgtgctctgc attggggaga aggaggtgtt cgtgctcgat
     7441 cgcgtcttcg acgcgttcct cgctgcgctc gaaaagaacg gcgcctgccg tctgtccggc
     7501 gcccaggtcg aacgtctcac ccaggccgcg ttcaccttcc tcccgggcca tggtggcgga
     7561 tgcgccgagc cggtcgtgaa cagagctctg atcggcaagg atgtccccgt tctcgcgcag
     7621 gcggccggca tcaatgcccc cgccggggcg ccgctcttgt tcgccgagac cgaggcgaat
     7681 catccattcg tccaggaaga gcagatgatg ccgttcctgc ccgtggtccg ggtgcgaagc
     7741 gtcgaggaag ggatcgaggg agcgcgcatc gccgagcgtg gctacaagca ctcggccatg
     7801 atccactcgc acgacgtcgc caacatgacc gccatggcca aagcgctcga aacgaccctc
     7861 ttcgtcaaga acgggtcgtg cctggccggc ctgggcaacg gaggcgaggg atacccgaat
     7921 ttctcgatcg cgacgaccac tggcgaaggc atctgcaacc cacgcacgtt cacccgcatg
     7981 cggcgatgcg tgatggtgga caagctcaga atcttctgaa gggcgcctct gtatgctcct
     8041 cgcccgggtc gaaggcagtc tcatcgccac gcgcaaacac gcgagtttcg acggctggcg
     8101 catggtgatc tgccagccca tcaacgcggc tggcggggcg gaaggcccgc cgcaggtggc
     8161 catcgatccg cacggagccg gcctgcatca gcgggtgatc atttcctccg acggcgccgc
     8221 cactcggtcg gctgtcggcg atccgagaag ccctgcgcgc tggctggtga tcggcgttgt
     8281 ggactcggag gcaggctgac atgagagtgg gcagcgtcat cggtcgcgtc acgttgagcc
     8341 agaccattcc cgccttctgc ggggcgcgat ggcttctgat cagcccattc acacgcgatc
     8401 acttccagca tcgccacaat ccgccaaccg gcttgagcaa ggatttctct ctggtggcct
     8461 atgacgattt gggcggcggt ctcggcgaca cggtcggata cgtcgaggga cgcgaagcgg
     8521 cgatgccctt cgcgcagccg acgccggtgg acgccatcac ggtggctatc gtcgacgatg
     8581 ttttctacac tcccctggca tgaaaaaagt ctacagtctg cgtgatgtcg aagagatgat
     8641 ccggcgtggc gaggatctga aggccattcc cggcgacgcc attctgacgc cctcggcgcg
     8701 cgacctcctg cgcgagcaaa tcgaccatcc ccgccccgcg acgcccctgc ggtcggctca
     8761 agctcccctc ccgacggacg tccgtccgtc gcccccggcg cgcccccaga cctcgaagag
     8821 cccccgagcg gagttggacg ccttcttcaa ttctccgtac tgccacgacc tgaagcagca
     8881 gatgtgcgaa gtgggccacc gcctctggca gcgggagtac gtcgacggca atggcgggaa
     8941 catagcgatt cgtgtcgggg aggacctggc gctctgcacg cccacactcg tcagcaaagg
     9001 ctcgctcaaa ccggaggaca tgtgcctggt ggatttcgag ggcgctcagt tatgcgggac
     9061 caagaagcgc accagcgaga ttctgatgca tctccagatg atgaagcgcc agccgctcga
     9121 tgtcgcgtcg gtgcattgcc atccccccta cgcaaccgcc ttcgcggtgg ccggcgtcga
     9181 gccgcccacc tgcatgattc ccgagtacga ggtgttctgc gccgtggcca tcgcccccta
     9241 ccgcacgccc ggatcgcccg agatgggccg cctggtcgcc gacctggtcg acaaacacaa
     9301 cacgatcctc atggccaacc acggcgtggt cgcctggagc cacaacagcg tcgaggaggc
     9361 ttactggcgc atcgaaatca tcgaggcgta ttgccgcacc ctgaccgtgg cggtgcaact
     9421 gggcaaaccg ctcaacacct tcacccccgg ccaactccag gacttgctca agatcaaaca
     9481 gaacctcggg ttcgtcgacc ctcgctacgg cctcaaggag tgcgaactct gcaacaacga
     9541 cgagtggcgc ccgggcgtca cgtgccagat tcccgcgcgc gaaacgatgc tgggactcga
     9601 tcccgaggcc gaagaactgg tccaggccgt cacggaccag atcgtggcca ggctgaaaaa
     9661 cgactagccc gcatgtctta caccccgacg gtgcagagga tagtctgact tcgcaagatg
     9721 actgctatgc gggctagccc gagccacaca aaaaggaccg atggatgaag tcattggacg
     9781 ccaaactcgc ggagatcaag gccaacccgg cttcccgcgc tttcattctc gccgatgcca
     9841 aggacgccga catggcgttc ggcgtgcggg ccatgggccc tcggcattac ctctcctcgc
     9901 acggagcgca ggccgcccgg ttctctcccg aagtctggac gcgtgaggag ttcgggcatc
     9961 gcaatctgcc cgagtttctc gacatcatcc gcgaggtcgt gggccagggc ctggtcgaca
    10021 tcatgctcat gtccgccagc gtgaacgagc aactggcgat caaggaaggg cttttccgca
    10081 agtcgcacgt cactccagcc gcccgcgcca acgacgcgac agatgtgtgg gccgtgcgcc
    10141 acggatcgta tctgcacgag ccgtcgcatc cgttccgcag cgcgaccatc gatcacattc
    10201 aatgcggcca ggcccagtgc gaccggagcg ccgacgatgt tccgggcgcc aacctgggtt
    10261 tgtactcgat caccttcgtc aacaatctcg agcaggatct ggcttcactc caggcgttca
    10321 aggagtttcg catcgaggcg gagcgcaaga aattccgcta tttcctcgaa gtcttcgatc
    10381 ccaacgtggc aaacggcatt ccacccgaaa agctcggcga gttcatcaac gacaacatcg
    10441 tgcgcagtct cgccggcgtc acaagcgccg gacgcccgct ctttctcaaa atcgcctatc
    10501 acggcccccg cctcatggag gagttggccc aatacgatcc caacctgatc gtcggcgtcc
    10561 ttgggggcag cgccggcacc acccacgacg ccttccggct catccacgac gcccagaaat
    10621 acggcgcgcg cgtcgccctg ttcggacgca agatcaacaa cgccgaacac cagctcgcct
    10681 tcatcgagat gctccgcctc atcaccgacg gccggattgc gcccgacgaa gccgtccgcg
    10741 cctatcacaa cattctgggc aagctgggtc tccagccaaa acggcctctc gagaaggatc
    10801 tccttctgac ggatcaggcc atgagctacg gcggcagccc gacgcgcgtc agcgtccccg
    10861 tcgcggccaa tccgctcgcc ccggcaatcg cgcccggagc cgctccgaag ccaccagccg
    10921 gccccgcggc caacgcaccc gcctggccgg tccgccctga cggttcgccc gacttcgcga
    10981 ccatgacctc cgcagagcgc cgcgcctacg atgcggacag gctcaaacgc cggttcgggt
    11041 gagccggcgg ctggttgcgc gttgcgcatc gaatgctgaa ggtctctgct tcagggtcct
    11101 cgcgcgcatt cgaccgtcga atgtcaatgc tctgccatga cgtgttgctt cggcaatagt
    11161 gcgtgagcgg caaatcggcg gcg
//
LOCUS       DAOQRO010000075        11166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5042_length_11166_cov_5.165962, whole genome shotgun sequence.
ACCESSION   DAOQRO010000075 DAOQRO010000000
VERSION     DAOQRO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5042_length_11166_cov_5.165962"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKK20_04355"
     CDS             complement(<1..783)
                     /locus_tag="PKK20_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNU99149.1"
                     /translation="MQYGLTIRSEGALDLISLGALVHRLDPGIIPFRKATECQIHVSG
                     GEFNVAANLADCFRLKTGIVTAMVDYPIGELIAERVKAMGVRPFYKTFAHDGVQGPNM
                     AAVYSDRGWGVRAPVVFYNRCNEAAARLKPGDFDWSSIFAGGVRWFHSGGIFAALSET
                     TAELIIEGMRAAKAAGAVVSFDLNFREKLWRISGGKDRAVAVLRRIVRNVDVLIGNEE
                     DLQKGLDIPGPEVAATSKLDPSAFFDMIERVLEQQPQVKVVAT"
     gene            942..3728
                     /gene="ppdK"
                     /locus_tag="PKK20_04360"
     CDS             942..3728
                     /gene="ppdK"
                     /locus_tag="PKK20_04360"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNU99150.1"
                     /translation="MPKALKSQKHVYLFGNKKADGDGSMKPLLGGKGANLAEMSRIGL
                     PVPPGFTITTEVCNYYYANKRTYPKQLQAQIKNGIEFIEKIMGTKFNDQTAMPLLVSV
                     RSGARDSMPGMMDTILNLGLNDRTVVALVNATKNERFAWDCYRRFVQMYGDVVMGVQK
                     RPNEDHEPFEVVIHELKHERYAQDIEDTRLTVDDLKELVKRFQVLIKDRTGGKEFPQD
                     PWKQLEGAVGAVFGSWMNDRAIVYRRKYNIPSEWGTAVNVQAMVYGNTGNTSGSGVAF
                     TRNPANGAKEFYGEFLINAQGEDVVAGVRTPEPVAQLAKEMPAAYKELDNIRTTLERH
                     FKDVQDFEFTIQDGKVFMLQTRNGKRTAMAALKFSMDMVKEKLITWETAVLRNPADQL
                     DQLLAPIFDAKAEKAAKVIATGLPAGPGAASGKLYLNADRADAAAARGEKVLLVRNET
                     SPEDLRGMIASEGILTAKGGVSSHAALVARQMGKVCVCGAAALQIDYNAKTVTAGGQT
                     FTEGDYLSINGTVGNVYVGQIPTAPSEIVQVLVDQTLDPKKSETYAMFKTLMDWCAKA
                     TRMQVRTNADTPEQAENAIAFGATGIGLTRTEHMFFEGDRIKAMREMILADNVADRKE
                     ALAKLLPYQKEDFVGIFRALKGFPATIRFLDPPLHEFLPHDDNPKGQQEIADEMDIPV
                     EKIRQRVKELHEFNPMLGFRGCRLGIVYPEISEMQARAVFEAAAEVQKSGIKVKPEVM
                     IPLVGFKRELDLQAEVVHRVAKEVMAEQKVRFNYSVGTMIEIPRGALTADEIAQTAEF
                     FSFGTNDLTQTCLGMSRDDSASFLGPYTEAEVVNKNPFASIDQTGVGQLVKIAVEKGR
                     STRPGIKLGICGEHGGDPDSVKFCHRVGLTYVSCSPFRVPVARLAAAQAAIAEKQAAG
                     AKTPAKKTAKKSRK"
     gene            complement(3948..5402)
                     /locus_tag="PKK20_04365"
     CDS             complement(3948..5402)
                     /locus_tag="PKK20_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99151.1"
                     /translation="MKLLRPTRLGLVLAWTTVSVVVAAQTDDTPTAHALVAAQQTLAD
                     PNWKAVADAVAAKHGNRVLSFTNSVFETLPALRAHFPRYVCFVAPPETVTRQFVTDVH
                     RLARRVDDDPYTDFFWGILTGYDAANALRIARQHEPLIIRRVASGTELPMDMVDEGVW
                     YCELNKNRMVRKEPGKTAVELEGPDDTTEALVKSLCEYQADLFVTSGHATERDWQIGF
                     RYRNGFFKHADGVLVGQDTRGNTFPIRSPNSKVYLPVGNCLMGHIDQRDCMATAWMNS
                     AGVCQMLGYIQPTWYGYMGWGVLDYFVEQPGRYTLTEAFFANHHALVHRLVTFFPELA
                     GADADAAGRTSVPIKVNEKAKAAGLTQNDARGLVFDRDFVAFYGDPGWAARMADRPKA
                     FDQRISMQDGTYVFEVMPRRGPDSFDAVNRNGSQRGGRPFVAYLPHRIRNAQVIEGAD
                     LEPVITDDFILVPNPRVCDPAKTYRVVFRAERIP"
     gene            complement(5577..6734)
                     /locus_tag="PKK20_04370"
     CDS             complement(5577..6734)
                     /locus_tag="PKK20_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNU99152.1"
                     /translation="MKSSACRIAALLAAVPIGFGSMLAGGQQTETQSSRCASEFIRVS
                     KDRTRFVLSNTQREFRPWGFNYDHDASNRLLEDYWEDEWATVSGDFEEMKALGANTVR
                     VHLQVARFMKSATEPDRGSLAQLGRLLRLAETNELYLDVTGLGCYRRKDTPSWYNDLD
                     EGRRWEVQARFWESVAEVCSASPAVFCYDLMNEPVVSEDKQNRDWTPGEFAGMCFVQR
                     LTLDRAGRSEKQVAKAWVETLVKAIRKKDQGRLITVGAIPWALTFPEAKPLFYSQEVG
                     AGLDFVSLHFYPKRGEVDKALAALAVYDVGKPIVIEEMFPLNCSTTELGAFIEGSRRL
                     AVGWLGFYWGKTIAEYRQSKRDIAASMALDWLEYFAAKTPDILGMSPKVER"
     gene            6942..7175
                     /locus_tag="PKK20_04375"
     CDS             6942..7175
                     /locus_tag="PKK20_04375"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99153.1"
                     /translation="MVGNPAAHTIRVKVRYGDFTTLTRQLTVEEPITEAKDMYRLACH
                     LLGRKKLVSRPLRLFGLGVSNLREQTAQQLILL"
     gene            complement(7182..8291)
                     /locus_tag="PKK20_04380"
     CDS             complement(7182..8291)
                     /locus_tag="PKK20_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99154.1"
                     /translation="MKTLSLTFCPLALVIAMLTFGVADARAASVQSAQEIIKYPDDRT
                     SARYRLDALDQGPVLRHGDGPGRCDHLGARDVWVWEHSGTYYMHYDGAGTNAWLACLA
                     TSTDLVHWTKKGAVLELGRKGDDDSASASYGVTFFDGKGWHMFYLGTPNTTPAPDYIP
                     AFPYLTMKARAASPTGPWTKQPDVIPFRTKPGTYYSATASPGHVIAHDGGYLMFFSAS
                     TDAPIRRTLGIARTKDLNGTWHIDSEPIVPLAEQVENSSLYYEPTSRTWWLFTNHVGL
                     RGGLEYTDAIWVYWTTTLDRWDPAHKAVVLDSKNCKWSRHIIGLPSVVQVGRRLAIFY
                     DGNAAEQMPTGVKSHMNRDVGLAWLDLPLVAPTAQ"
     gene            complement(8288..8767)
                     /locus_tag="PKK20_04385"
     CDS             complement(8288..8767)
                     /locus_tag="PKK20_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99155.1"
                     /translation="MNESIIPPPRTIRDELEKLVPADLHGPAGGPDEELDEAGVSERY
                     LVGMLAPKRNPAVVDLFDELAVGGKGTDEEGMVDIAPPQAESLIPSSFGLTFAVSAQA
                     KALRIAARWGRYLRTDSETQTNEKSNPKKVWKRTQRQGVFDPIPLVSGPMKNLIQTP"
     gene            complement(8952..9155)
                     /locus_tag="PKK20_04390"
     CDS             complement(8952..9155)
                     /locus_tag="PKK20_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99156.1"
                     /translation="MEPILTALNLFRDGTITNAAEVLFGRNPAQQFPQVRVRVTVYAM
                     DKGGDFVDNQDRHPAKPGQIRPE"
     gene            complement(9465..10457)
                     /locus_tag="PKK20_04395"
     CDS             complement(9465..10457)
                     /locus_tag="PKK20_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99157.1"
                     /translation="MNAATAAYLGRIAFGPPQTHRNIVVFPLLGPDAGPAPYLTLGEA
                     LAGHCLTVTEVSEGGSVPELKVISQSDRPILLLDGEELVGAKQNRVLNTTILLKERAE
                     TTIPVSCVEQGRWSYASAHFADSGAVMSRKSRASKVRSVSESLAAGAHYSSDQGEVWR
                     EVAALACSAGVNSPTHAMQDVFKARAAELEACARVLAPTAGQTGLFVVIDGEVAGFDF
                     IAHADAYAKLHPKLLKSYVIGALIEPKPGAVEVAKAAAAAQAFLHEAGESDEKAFESV
                     GYGRDYRYQKPGLAGSALVHEDRVVHMAFFRLPKGESAGSSMASLRQRRRRFSE"
     gene            complement(10930..>11166)
                     /locus_tag="PKK20_04400"
     CDS             complement(10930..>11166)
                     /locus_tag="PKK20_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99158.1"
                     /translation="CLYSPERVSADSRPRLRDASALFAATLAHGIREGADAQATFTFA
                     QEHAQRHVQSASVREALLAPATRAVLAGGRPGAG"
BASE COUNT         2163 a         3561 c         3398 g         2044 t
ORIGIN      
        1 ggtggcgacg accttgacct gcggctgctg ctcgagcacg cgttcgatca tgtcgaaaaa
       61 cgcgctcgga tcgagcttcg acgtggccgc cacctcgggt ccgggaatgt ccaggccctt
      121 ttggagatcc tcttcattgc cgatcagcac gtccacattt ctcacgatgc gccgcagaac
      181 cgcgaccgcg cgatccttgc cgcccgagat tctccagagc ttctcgcgga aattgaggtc
      241 gaacgacacc acggcgcccg ccgccttcgc cgctcgcatg ccttcgatga tcaactcggc
      301 cgtggtctcg gacagggccg cgaagattcc gccgctgtgg aaccaccgca cgccgccggc
      361 gaagatgctg ctccaatcga agtcgcccgg tttgaggcgc gccgccgcct cgttgcagcg
      421 gttgtagaaa accacgggcg cgcgcacccc ccatccgcga tcgctgtaga ccgccgccat
      481 gttcggcccc tggacgccgt catgggcgaa tgtcttgtag aacggacgca cacccatcgc
      541 cttcacccgc tcggcgatca gttcgccgat gggataatcg accatcgccg tcacaatccc
      601 ggtcttcaga cggaagcagt cggcaaggtt cgccgccacg ttgaactctc cgccgctgac
      661 atgaatctga cactcggtcg cctttcgaaa cgggatgatg ccaggatcga gtcgatgcac
      721 aagggcgccc agtgagatca ggtccagcgc gccttccgaa cgaattgtca agccgtattg
      781 catgggatgg attagagcag atgcaccccg cggcgagaag ctttgtttgc gtacggctgt
      841 cgtcaaaagc gccgttgaca cgcccctttc ctttgttagc gtctcggcca ccttcgcaat
      901 caatgaacca gcgaaaccga gtaaacgaca aagaacaact tatgccaaaa gcactgaaat
      961 cacagaaaca cgtctatctg ttcggcaaca agaaggccga cggcgacggg tccatgaaac
     1021 cgctcttggg cggcaagggc gccaatctcg cggaaatgtc ccgcatcggc ttgccggtgc
     1081 ctcccggatt caccatcacg accgaggtgt gcaactacta ctacgcgaac aagcggacgt
     1141 accccaagca actccaggcc cagatcaaga acggcatcga gttcatcgag aagatcatgg
     1201 gcacgaagtt caacgatcag accgccatgc cgcttctggt ttccgttcga tccggcgcgc
     1261 gcgattcgat gccgggcatg atggatacga ttctcaacct gggtttgaac gaccggacgg
     1321 tggtcgccct ggtcaacgcc acgaagaacg agcggttcgc ctgggattgt taccgccgct
     1381 ttgtgcagat gtacggcgac gtcgtcatgg gtgtacagaa gcgccccaac gaggaccacg
     1441 aaccgttcga agtagtgatt cacgaactca agcacgagcg ttatgcgcag gacatcgaag
     1501 acacccggct gacggtggac gatctcaagg agttggtgaa gcgtttccag gtcctcatca
     1561 aggaccgcac tggcggcaag gagttcccgc aagacccgtg gaagcagctc gaaggcgcgg
     1621 ttggcgccgt gtttgggtcc tggatgaacg accgggcgat cgtctaccgc cgtaagtaca
     1681 acatcccctc ggaatggggc acggccgtca acgtgcaggc gatggtttac ggcaacaccg
     1741 gcaacacctc agggtcgggc gtggcgttca cgcgcaatcc ggccaacggc gcgaaggaat
     1801 tctacggcga gttcctgatc aatgcgcagg gcgaggacgt cgtggccggc gtgcgcacgc
     1861 ctgagcccgt ggcgcagttg gcgaaggaga tgcccgcggc gtacaaggaa ctcgacaaca
     1921 tccgcacgac gctcgagagg catttcaagg atgtgcagga cttcgagttc acgatccagg
     1981 acggcaaggt cttcatgctt cagacgcgca acggcaagcg cactgccatg gcggcgctga
     2041 agttctcgat ggacatggtc aaggagaagc tcatcacctg ggaaacggcc gtgctgcgca
     2101 acccggccga ccagctcgat cagctcctgg ccccgatctt cgatgcgaag gcggagaagg
     2161 cggccaaggt catcgccacc ggcctgcccg ccggccccgg cgctgcgagc ggcaagctct
     2221 acctgaatgc cgatcgcgcc gatgccgccg cggccagggg tgagaaggtg ctgctggtgc
     2281 gcaacgagac gtcgcccgaa gacctgcgcg gcatgatcgc gtccgagggc atcctgaccg
     2341 ccaagggcgg cgtgagctcg cacgcggcgc tggtggcccg ccagatgggc aaggtctgcg
     2401 tctgcggcgc cgccgctctg cagatcgact acaacgccaa gaccgtcacc gcgggcggac
     2461 agaccttcac ggaaggcgat tacctctcga ttaacggaac ggtcggcaat gtctacgtcg
     2521 ggcaaatccc gaccgccccg tctgaaatcg tgcaggtgct cgtcgaccag acgctcgatc
     2581 cgaagaaaag cgagacgtat gcgatgttca agacgctcat ggactggtgc gccaaggcca
     2641 cccgcatgca ggttcgcacc aatgccgaca cgccggagca ggccgagaac gccatcgcgt
     2701 tcggcgccac gggcatcggc ctcacccgca ccgaacacat gttcttcgag ggcgaccgca
     2761 tcaaagcgat gcgcgagatg atcctcgcgg acaacgtggc ggaccgcaag gaggcgctcg
     2821 ccaagctcct cccctaccag aaggaggact tcgtcggcat cttcagggcc ctcaagggct
     2881 tccccgccac catccggttc ctcgatccgc cgctgcacga gttcctgccc cacgacgaca
     2941 accccaaagg ccagcaggag atcgccgacg aaatggacat accggtcgag aagatcagac
     3001 agcgcgtcaa agaactgcat gagttcaacc cgatgctcgg cttccgcggc tgccgcctgg
     3061 gcatcgtcta tcccgaaatc tccgagatgc aggcccgcgc ggtcttcgag gcggctgccg
     3121 aggtccagaa gtcgggcatt aaagtgaaac cggaagtcat gatcccgctg gtcggtttca
     3181 agcgcgaact cgatctccag gccgaagtcg tccaccgcgt cgccaaggaa gtcatggccg
     3241 agcagaaggt gcggttcaat tacagcgtcg gcaccatgat cgaaatcccc cgcggcgcac
     3301 tcaccgccga cgagatcgcc caaaccgccg aattcttcag cttcggcacc aacgacctca
     3361 cacagacctg ccttggcatg agccgcgacg actcggcgag cttcctcggg ccttacaccg
     3421 aagccgaagt cgtgaacaag aacccgttcg cctcgatcga ccagaccggc gttggccaat
     3481 tggtcaagat cgcagtcgaa aagggccggt cgacacgccc cggaatcaag ctcggaatct
     3541 gcggcgaaca cggcggcgat cccgactcgg tcaagttctg tcacagagtc ggcctgactt
     3601 acgtcagttg ctcgccattc cgcgtccctg tcgcccgtct cgccgccgcc caggctgcga
     3661 ttgccgagaa acaggcggcc ggcgccaaga cgcctgccaa gaagacggcc aagaagagca
     3721 ggaagtagcc tgccggtctc agatcaggac tccggcgagt tcctgaccgc acgattcgca
     3781 gccgctccgg cacaccgggg cggcttcttc ttttggattc gacgccagcc ggcgccctga
     3841 cttgaaccgc ggagattcgg agcgcggcgg ctcttgcgag tccgcatttc agttcaaagt
     3901 gacagtggcc ggtcggatga caccaacagt tggtgagaag tgcagggcta gggtattcgc
     3961 tcggccctga aaacgacccg ataggttttc gccggatcac acacccgcgg gttcggcacc
     4021 aggatgaaat cgtccgtgat gaccggttcg aggtccgcgc cctcgatgac ctgcgcgttg
     4081 cggatgcggt gcggcaggta agccacgaac gggcgcccgc cccgctgcga accgttccgg
     4141 ttgacggcat cgaagctgtc cggaccgcgc cggggcatga cttcgaacac ataggtccca
     4201 tcctgcatgc tgattctctg atcgaaggcc ttcgggcggt cggccatccg cgccgcccac
     4261 ccgggatcgc cgtagaacgc aacgaaatcg cggtcgaaca ccagcccacg cgcgtcattc
     4321 tgcgtgagtc cggccgcctt cgccttctca ttgaccttga tcggaaccga cgtgcgtccg
     4381 gctgcgtccg catccgctcc agccaattcg ggaaagaacg tgaccaggcg atggaccagg
     4441 gcgtggtgat tggcaaagaa cgcctcggtt agcgtgtagc ggcccggttg ctcgacgaaa
     4501 taatccagca ccccccaccc catgtagccg taccacgtcg gttgaatgta accgagcatc
     4561 tggcagacgc cggcgctgtt catccacgcc gtcgccatgc aatcgcgctg gtcgatgtgc
     4621 cccatgagac agttgccgac cggcagatac accttcgaat tcggcgagcg gatggggaac
     4681 gtgttgcccc gcgtgtcttg gccaacgagc acgccatcgg cgtgcttgaa gaagccgttt
     4741 cgatacctga acccgatctg ccagtcgcgt tccgtggcgt gacccgaggt cacaaacaaa
     4801 tccgcctgat actcacagag cgacttcacc agcgcctcgg tggtgtcgtc cggaccctcg
     4861 agttcaacgg ccgtcttgcc cggctccttt cgaaccatcc tgttcttgtt gagttcacag
     4921 taccagactc cctcgtcgac catgtccatc ggcagttcgg ttcccgacgc gaccctgcgg
     4981 atgatcaatg gctcgtgctg cctggcaatg cgcagggcat tcgccgcgtc atagcccgtg
     5041 aggatgcccc agaagaaatc ggtgtacgga tcgtcatcga ctcggcgcgc cagacggtgg
     5101 acatcggtta caaactgtcg ggtgacggtc tctggagggg ccacaaaaca gacgtaccgc
     5161 gggaagtggg cccgcagcgc gggcagtgtc tcgaacaccg agttggtgaa cgacagcaca
     5221 cggttgccat gcttcgccgc caccgcatcg gcgacggctt tccaattcgg atcggccagc
     5281 gtctgttgtg ccgccacaag cgcgtgggcg gttggcgtat catcggtctg ggctgccacg
     5341 accacgctca ccgtggtcca ggcaagcacc agtcccagcc gggtcgggcg cagcaacttc
     5401 atggggggga gtctgggctt tggtcctcac acttccaacc caaaaacaaa cagagccgat
     5461 ccgctccaaa accgggtcat tttcccaatg gagggtgtca atggatgtca gacccgccgg
     5521 agctccagcg gtccctggaa tcgtccgtag atgacagcaa gcatgttgca gaaagctcag
     5581 cgctcgactt ttggggacat gcccagaatg tccggcgtct tcgcggcaaa gtactcgagc
     5641 cagtccaacg ccatcgacgc ggcgatgtcc cgtttgctct gccggtattc ggcaatggtc
     5701 tttccccaat agaatccgag ccagccgacc gccagcctgc gcgatccttc gatgaacgca
     5761 cccaattccg tcgtcgagca attcaatgga aacatctcct cgatgacgat cggcttgccc
     5821 acatcgtaga ccgcaagtgc cgccagcgcc ttatccacct cgccccgctt gggatagaag
     5881 tgcagactga caaagtccag accggctcca acctcttgcg agtagaacag cggcttggcc
     5941 tcggggaagg tcagcgccca agggatcgcg ccgacggtaa tgaggcgccc ttgatccttc
     6001 ttcctgatcg ctttgaccag cgtttcgacc cacgccttgg ctacctgctt ttcgctgcgc
     6061 ccggcgcgat cgagggtcag gcgttgcaca aagcacatgc ccgcgaactc gcctggcgtc
     6121 cagtcccggt tctgtttgtc ctccgaaacg accggctcgt tcatcaggtc gtaacagaac
     6181 acggccgggc tggccgagca gacctcggcg accgattccc agaaacgggc ttgaacctcc
     6241 caccgtcggc cttcgtccaa gtcattgtac caactcggcg tatccttcct ccggtagcag
     6301 cccaggccgg tgacatcgag gtaaagctca ttcgtctcgg ccaaacgcag cagacgacca
     6361 agctgcgcga gcgatcctcg atccggctcg gttgccgatt tcatgaacct ggcgacttgc
     6421 agatggacac gcacggtgtt cgcgccgagg gctttcatct cctcgaaatc tccgcttacg
     6481 gtcgcccact cgtcctccca gtaatcttcc agaagcctgt tcgaggcgtc gtggtcgtaa
     6541 ttgaagcccc acggtctgaa ctcgcgttga gtgttcgaga gaacgaaccg cgttcggtcc
     6601 ttgctgacgc ggatgaactc ggacgcgcat cgggaggact gtgtttcggt ttgttggccg
     6661 cccgccagca tcgagccgaa gccgatcggc acagccgcca gcaacgcggc gattctgcac
     6721 gcggaggatt tcatcaagat gagttctttt gccggattcg tcacacgtgc caaacgaatg
     6781 ccggacggag gtatagaatt gggaatgcga tcagagcaac acgaaatcct ccacggcgta
     6841 acagttctcc cctgtcctgc acaccggcgg ttcgatcatc ctgacccaaa cccggccaca
     6901 cattgaacgg ccttcaaatc gcaggctgat ctgccgtcac aatggtcggc aaccccgccg
     6961 cacacacgat tcgggttaag gtgcggtacg gcgacttcac gaccctgacg cgccaactca
     7021 cggtcgagga accgatcacg gaagccaagg acatgtatcg gcttgcgtgt cacctgctgg
     7081 ggcgaaagaa gctggtgtca cgtcccttgc ggctcttcgg tcttggcgtc agcaacctgc
     7141 gcgagcagac ggcgcaacag ttgatcctgc tgtaggccgg gtcattgagc cgtcggcgcc
     7201 accaacggca ggtcgagcca ggccagaccc acgtcgcggt tcatgtggct cttgacgccg
     7261 gtgggcatct gctccgccgc gttgccgtcg tagaagatcg ccagccgccg ccccacctgg
     7321 acgaccgacg gcaagccgat gatgtgccga gaccatttgc agttcttcga gtccaggacg
     7381 accgctttgt gcgccgggtc ccagcggtcg agggttgttg tccagtaaac ccaaatcgcg
     7441 tccgtgtact ccaagccgcc gcgcaacccg acatggttgg taaacaacca ccaggtccga
     7501 ctcgtcggct cgtagtacag tgagctgttc tcgacttgct cggccaacgg aacgatcggc
     7561 tcggagtcga tgtgccaggt gccgttgagg tccttggttc gggcaatgcc cagggtccgc
     7621 cggatcgggg catcggtgga ggcgctgaag aacatcaggt aaccgccgtc gtgggcgatg
     7681 acgtgcccag ggctggcggt tgcggagtag tacgtgcccg gcttggtgcg gaacgggatc
     7741 acgtccggct gcttggtcca cggtccggtg ggtgaagccg cccgcgcttt catcgtgagg
     7801 tacgggaagg cggggatgta gtcgggcgcc ggggtcgtat tcggcgtccc gagatagaac
     7861 atgtgccagc ctttgccgtc gaagaacgtg acgccatacg aagcggaagc ggagtcgtcg
     7921 tcgcctttcc ggccaagttc gagcacggcg cctttcttcg tccagtggac caggtctgtg
     7981 ctcgtggcca ggcaggcaag ccaggcgttg gtgcccgcgc cgtcgtaatg catgtagtat
     8041 gtcccgctgt gttcccagac ccagacgtca cgggcaccaa gatggtcgca tcgacccgga
     8101 ccgtcgccgt gccgcaatac cggcccctga tccagggcgt cgaggcgata ccgggcagac
     8161 gttcggtcgt cggggtactt gatgatctcc tgggccgatt gcactgaggc cgcgcgggcg
     8221 tcagccacgc cgaaggtcag catagcgata accagggcga gcgggcagaa ggtcaagctg
     8281 agtgtcttca tggggtttgg attaggttct tcatggggcc ggaaacaagc gggatgggat
     8341 caaagacgcc ttggcgttgg gtccgcttcc agaccttctt gggattgctc ttctcgttgg
     8401 tctgcgtctc gctgtcggtg cggaggtaac ggccccaccg cgccgcgatc ctcaacgcct
     8461 tggcctgcgc cgataccgcg aaggtcaggc cgaaggacga cgggatcagc gattccgcct
     8521 gtggcggtgc gatgtctacc atgccttcct cgtcggtacc tttgccgccg accgccagtt
     8581 catcaaacag gtccacgacc gccgggttcc gcttgggtgc caacatcccg accagatacc
     8641 gctcgctgac accggcctcg tccagttcct cgtcaggccc gcctgccggg ccatgcagat
     8701 cggccggcac gagtttttcg agttcgtctc ggattgtcct gggtggcggg atgattgatt
     8761 cgttcatgcg actcctatgc ctgccggacc ggaaccggtc cgccctgcgc tacatggccg
     8821 gatttggccg ggtttggccg gattcatagt tggccctccg cgagccaccc tcactgaacc
     8881 ttcgacaaac gcttgatttg caggcttaac cgactcagcc gaggaattca gagtatggtg
     8941 aatgctcgtt ttcattctgg ccggatttgg ccgggtttgg ccgggtgccg atcttggttg
     9001 tccacaaagt cgccgccttt gtccatggca taaaccgtga cgcgcactcg tacttgggga
     9061 aactgctgcg ccggattccg cccaaagaga acctctgccg cattggtgat cgttccgtcc
     9121 cgaaacaggt tcagcgctgt caaaatcggt tccacttggt tgcccagcga gagttgccgc
     9181 tgttcgcgtg cctgttgtgc ggtgctgacg atcaagtccc aggactcccg ctggtgccag
     9241 gaaaccctga ttttgacggt gagcctacgc ggccttttcc acggaaacca gaacaaatcc
     9301 ggcgcagccg gtcagagcac tttggagtgc cgcacgaacc atcctgggtc gtaccaaggc
     9361 ggagtcggcg ctgtgcgtgg tggccgggac ttgctcgccc gcccgaatca accggtgagc
     9421 aggcaacccc gcattgttcc gtagctgagt ttcaatgctt gtcctcattc gctgaaccgc
     9481 cgacggcgtt gccgcagaga ggccatcgag ctgccggcgg attccccttt cggcaacctg
     9541 aagaaggcca tgtgcaccac gcggtcctcg tggaccaacg ccgacccagc caatcccggt
     9601 ttctggtagc ggtagtccct gccgtagccg accgattcaa aggccttctc gtcgctctcg
     9661 ccggcttcgt gcaggaaggc ctgggcagcg gcagcggctt tcgcgacctc gactgcaccc
     9721 ggcttgggct cgatcagggc gccgatcacg taactcttca gcagtttcgg gtgcagtttg
     9781 gcataggcgt cggcgtgggc gatgaaatcg aatccggcca cctcgccgtc gatcactacg
     9841 aacaggcctg tctgcccggc agtgggtgcg agaacgcggg cacacgcctc gagctcggct
     9901 gcgcgggctt tgaacacgtc ctgcatagca tgtgtgggag agttcacacc ggcgctgcag
     9961 gccagagcgg cgacctcgcg ccagacttct ccctggtcag acgaatagtg cgcaccagcg
    10021 gcgagcgact cggaaacgga tcgcaccttg gacgcgcggc tcttgcgcga cattaccgcg
    10081 cccgaatcgg cgaaatgagc ggaggcgtac gaccaccgtc cctgctcgac acaactgacc
    10141 gggatcgtgg tctcggcgcg ttccttgagc aggatggtgg tgttcagcac ccggttctgc
    10201 ttggcgccca cgagctcttc gccgtccaga agcaggatgg gccggtcgct ttggctgatg
    10261 accttgagtt cgggcaccga accgccttcg gagacctcgg tcacggtgag gcagtgcccg
    10321 gccagggctt cgccgagcgt caggtacggc gcggggcccg catccgggcc gagcaaagga
    10381 aagacgacaa tgttgcggtg tgtttgcggt gggccgaaag cgattcgacc gagataagct
    10441 gcggtagctg cattcatgtt ttttgccttt ccaggtgcgg cgactcggaa ccgacgcacc
    10501 ggcgcaccga cgcaccgacg caccgacgca ccggcgcacc gacgcaccga cgcaccgacg
    10561 caccgacgcc cccacctccc ggcctggcga gcctcgccaa gatcgtttcg tactgttcgc
    10621 ctgcaagatt acgcgagggg gtctgacatc cgctgacacc ccctcataca aagttttggc
    10681 agaattgcag cgggtggcgg cgtttggggg acgggttgat ggggtgtggt tgagggttga
    10741 cgagcaaccg gttggacgtt ggatgttgag cgttgcacgt tgtgcgttaa ctatttagac
    10801 gatttcccaa tcgccgatcg accgggaaac gggcctcgcg tcggctgccg atgagacctg
    10861 aacctccaac gtccaacgtc caacgttcaa cgcccaacgg tcagttcacg gccggccaac
    10921 aggaggcgtt cagccagcac cagggcgtcc accggccaaa actgctctgg tcgcgggcgc
    10981 caacagcgcc tcccgaacgg acgcggactg cacatgcctc tgcgcgtgtt cctgggcgaa
    11041 cgtgaacgtc gcttgggcat cggcgccctc ccggatccca tgagccagtg tggcggcaaa
    11101 gagggcgctg gcgtcgcgta gccgcggacg ggagtccgcg ctgactcgct ccggcgaata
    11161 cagaca
//
LOCUS       DAOQRO010000076        10972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5207_length_10972_cov_5.461116, whole genome shotgun sequence.
ACCESSION   DAOQRO010000076 DAOQRO010000000
VERSION     DAOQRO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5207_length_10972_cov_5.461116"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1054
                     /locus_tag="PKK20_04405"
     CDS             233..1054
                     /locus_tag="PKK20_04405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99159.1"
                     /translation="MALGLLAKGEWRRVGLALYARIYVGVHWVLFPLYRPFIRRECGP
                     VCDTVLKVETKHPVAFDSPDHAVPHGTRYNNSTNRKFVLLMHRHFRRRSGRGRLSMMD
                     LGCSGGQLVADFAALGWIAVGLEGSDYSLKHRRANWAGLANRNLFTCDIAKPFTVLRD
                     GERLECELITAWEVLEHIHRDGLEPLFANIRSHLTEGGLFIASTSSSPSVVDGVELHQ
                     TRMSNAEWRAWVESRCADLEPVDLGLKTYQYVRFDFGEQSFLVYRKRSAQGGRAE"
     gene            1257..2171
                     /locus_tag="PKK20_04410"
     CDS             1257..2171
                     /locus_tag="PKK20_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99160.1"
                     /translation="MAIDTEAARLLVAGMKSGIPFTNTITLGRQSYLPGNRESRQLLI
                     DNGIDPARHPELFAGRQGERYAEPFFRLLGAHRIESLDASDFEGATTVHDLNQPIASE
                     LKGQFDVVYDGGTIEHVFNFLVAIRNCMELVRVGGRVILHTTANNYFGHGFYQYSPEL
                     FFRIFTADNGFSVERLIAMEYGPRRRWYEVSDPASIKARTPLINACPVLLFVQARKDA
                     QRPLFEQVPQQSDYSNMWQGCAARGKSGSAPHECAPTSAGARFKRALIERAPASARML
                     EAFKFSNWNRAFSFRNRAAFKPVNKRRL"
     gene            complement(2260..3096)
                     /locus_tag="PKK20_04415"
     CDS             complement(2260..3096)
                     /locus_tag="PKK20_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99161.1"
                     /translation="MLRIFEMRGETMKPSRQAVVTVASGQHTEILDRTFTSFGQNPFL
                     ELHAFILGERLPQRQLPGIVYHLREPDPTYQHFLREIYYRRFALIDELDCDYALIVDN
                     HDVLCLQPIPEIPALLRGAAVGACVEHAGGKYIAGQGYTSSYVNAGVTFWNIAASRRI
                     RADVVARGRARFRSIEDQLTLNEVVHTRYYDDLILLPCQYNYRPCLAPTRVRGWPTVS
                     HLDGVRIYHNVYSLDAARKLIPVAARAALETLPPDGRALTPFQRFRRRVQHRLFYRHQ
                     VR"
     gene            3388..3627
                     /locus_tag="PKK20_04420"
     CDS             3388..3627
                     /locus_tag="PKK20_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3424..3426,aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HNU99162.1"
                     /translation="MQAEIVIEYCVAUNYEPHAVGLAAQLLGLKFKIGSLKLIPSSGG
                     VFEVSVNGRNVYSKKATGQFPDPEAVMRDVRAMLK"
     gene            complement(3853..4782)
                     /locus_tag="PKK20_04425"
     CDS             complement(3853..4782)
                     /locus_tag="PKK20_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNU99163.1"
                     /translation="MKHYIFIDYATQAYIALVGVLILLFHNQTVPCWPCLVAVHAACL
                     VLVHLLIQSHARWPSNRFLDFLRHFYPVLLYTGFYRETGMLNHMFAGDFLDVHFIRLE
                     ESLFGLQPCLWLMDAFPYLAVSEVLYASYFSYYIMIVGVGLALFVRRRLQFYHYVSVV
                     SFVFYLCYLTYICLPVMGPRAFFRGIGGFRLPDAIQASAGPLAYPDAVTHGVFFNIMA
                     WIYRNFESPGAAFPSSHVAIALCTVWFSFRYLPRIRWLHLVVALLLCISTVYCRYHYV
                     VDVVAGALTVAILLPLANRLYHRFQARGEAIAE"
     gene            complement(4779..5774)
                     /locus_tag="PKK20_04430"
     CDS             complement(4779..5774)
                     /locus_tag="PKK20_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNU99164.1"
                     /translation="MNVLLTGGNGFLGSHILDALCARDIPAVLLARPTGNLQFVRDHL
                     PRVEVRQGSVTDPASLREALSGITHVIHCAGATKALRLSEFDAVNRGGTAHLVDALAA
                     RPGQIRRLILVSSLAACGPGTAQAPARETDTPHPVSEYGRSKLAAEREVTERCRVEYA
                     ILRPPAVYGPRDREFLRLFRTVQRGWAPAIAGGRQPLSLVYGADVASAAIACLDHPAA
                     AGKVFHVAGDEVATGTQVTELIARALGCRVKQVPVPGLLVRLVCAAATAGARLSGRAS
                     LLAHDKHRELLAPGWVCDAGRLQRELGVRCPTPLSAGAVSTLAWYREHRWMDWNS"
     gene            complement(5785..7107)
                     /locus_tag="PKK20_04435"
     CDS             complement(5785..7107)
                     /locus_tag="PKK20_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNU99165.1"
                     /translation="MKRTDHKEELEEQAHSDAPEDADAVPDAAPAESRQEARSARSPS
                     KHVPRLLEKTRAFRNAEEVRRLGLYPYFRTISSAQDTEVTIGERKVLMLGSNSYLGLT
                     NHPKIKEAAKAAVDKYGGGCAGSRFLNGTLDIHLELEEALARLVQKEAVLLYSTGFQV
                     NLGVISSLVGKGEYVLADKSDHASIVEGCLLCQGKFVRFAHKDMAALENRLQMIERET
                     GKLIVVDGVFSMEGDIIQLPDLCKLAREYGTVVMVDDAHAIGVLGDRGAGTANHFGLV
                     DEVHLIMGTFSKSLASLGGFIASDKATIDYLKHHSKPLIFSASMSPANVAAVLAALKI
                     MTGEPERIQRLWHNTDRMKQGLLALGFDLGASQTPILPVYVRDLLTTFKFCRQLEEQG
                     VFVNPVVSPGVPPGQELLRISLMATHTDNQIDFALEMMDRVGRELGVI"
     gene            complement(7131..9257)
                     /locus_tag="PKK20_04440"
     CDS             complement(7131..9257)
                     /locus_tag="PKK20_04440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNU99166.1"
                     /translation="MSRVLATGVGALSLASGLAASEVPDAGPLASEWKGPYGGVPPFD
                     RVEVAHFKPAFETAIGAYRAEIRQIADQSAPANFDNTIAALEKAGRALDRVEAVYGVW
                     GSTMSTPEFRAIEQEMEPKLAALRDEVFQNENLFKRIEAIYQSPDKARLTSEQQRLVW
                     LHYTEFVRAGARLDAAGKARVATINQRLATLYTTFSQNLLADEEGYATVIADAAGLAG
                     LPESFRAAAAAEAEGRGQKGQWVIANTRSSVEPFLTYSTRRDLREQVWHNFVSRGDRP
                     GAHDNKALIPEILGLRAERARLLGYATHAHWRLENSMARTPERAMELMEAVWKPALAR
                     VREEVADMQAIADAEGAGIRIAPWDYRFYAEKVRKAKYDLDENEVKPYLQLDKLREAM
                     FWAAGQIYGLTFTPAADIPVYHGDVRVWEVRGADGRHVGLFYFDPYARTGKRSGAWMN
                     AYRNQERFERPITTLVSNNSNFMKGGAGEPVLVSWEDAKTLFHEFGHGLHGLCSDVTY
                     PSLSGTRVPRDYVELPSQINERWLATPEVLNRFALHSKTGEPMPAALAERIQKASKFN
                     QGFATIEFLASGLIDMRLHLAGERAIDPAAFERDELARLGMPSEIVMRHRMPHFAHIF
                     AGEGYSAGYYSYLWADMLTADAAEAFLEAGGFYDKSIAARFKRHVLSAGNTVEPTEAY
                     RQFRGRDAGIAALMRDRGFPVPAGAK"
     gene            complement(9842..10942)
                     /gene="ilvC"
                     /locus_tag="PKK20_04445"
     CDS             complement(9842..10942)
                     /gene="ilvC"
                     /locus_tag="PKK20_04445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNU99167.1"
                     /translation="MATIDFGGTREQVITRKEFPMAKARKVLKNEVIAIIGYGVQGPA
                     QSLNLRDNGFNVIVGQASQFKRDWDRAVQDGWKPGKTLFEIDEAVRRATIVQILVSDA
                     AQRAVWPVIKANLKPGDALYFSHGFSIAYRKQTGVVPPADVDVIMVAPKGSGTSVRRN
                     FLTGAGINSSFAVEQDATGRAEERCLAIGIGIGSGYLFPTTFEHEVFSDLTGERGVLM
                     GALAGIMEAQYDVLRANGHTPSEAFNETVEELTQSLIRLVDENGMDWMYTNTSATAQR
                     GALDWKPRFKKAVLPVFKELYQRVKTGKECARVLKACGTPNYQAQLQKELRAIRDSEM
                     WLAGAAVRALRPKEPARTIAKGIKGIAGRAAN"
BASE COUNT         2064 a         3454 c         3502 g         1952 t
ORIGIN      
        1 cccacaccga ttcagggcgg aacacagatt cccgaagagg acaagttctt cctcaacaac
       61 agcaaccctt caggaattta aatgaaataa ccgtgtgtgt ggaagggacc cgtagtacat
      121 tcgtcttgcg tcggcggcgc aaccccttaa gtttttgcgc ctgcgggcct tgcaccccat
      181 tgggcagctc tggatcatgt tctatataag gtactttttg agaaacgtcg ctttggccct
      241 tgggctcctg gccaagggcg aatggcggcg agtggggctg gcgctctacg cgcgcatcta
      301 cgtgggagtg cattgggtgt tgtttccgct ctatcgccct ttcatcaggc gggagtgtgg
      361 gccggtttgt gacaccgtgt tgaaggtgga gacgaagcac ccggtggcgt tcgacagtcc
      421 ggaccatgcc gtgccgcatg ggacgcggta caacaacagc accaatcgga agttcgtgct
      481 gttgatgcat agacacttcc gccggcggag cggccgaggc aggctgtcga tgatggatct
      541 gggctgttcg ggtgggcagt tggttgccga cttcgccgcg ctgggctgga tcgccgtggg
      601 tttggaaggt tcggattatt ccctgaaaca ccgtcgtgcc aactgggccg gcctcgccaa
      661 caggaacctc ttcacctgcg atatcgccaa gccgttcacg gtgctgcggg acggggagag
      721 actcgagtgc gagttgatca cggcatggga agtcctcgaa cacatccaca gggacggcct
      781 cgagccgttg ttcgccaaca ttcgatcgca tctgaccgag ggcggcctct tcatcgcctc
      841 gaccagttcg agtccgagcg tagtcgacgg cgtcgaactg caccagacgc gcatgtcgaa
      901 cgccgaatgg cgggcctggg tggaatcgcg ctgcgccgac ctcgagccgg tggatctcgg
      961 gctgaagacc tatcagtatg tgcggttcga tttcggtgaa cagtcgtttc tcgtctatcg
     1021 gaagcgatcc gcacagggcg gacgcgcgga gtaatcgcgc ggcccgcatc ctggcccggt
     1081 cccgcggggc ttcacatcac gccggcccgg cgcggttgtg ctttgctagc gcgcatgaat
     1141 gcaccttccg atgacatcgg cgccgcgttc ttcagaagtt ggcgagaaat gtgggctagc
     1201 gccgggcctt tttgcttgct ccagggtcgg cgcgttccca acaatcaaat caacttatgg
     1261 ccatcgacac agaagcggca cggctgctcg tggcggggat gaagtcgggc attccgttca
     1321 ccaacaccat cactctgggt cggcagagct acctccccgg gaatcgagaa tcgcgccagt
     1381 tgctcattga caacggcatc gatccggccc ggcatcccga gttgttcgcg gggcggcaag
     1441 gggaacgcta cgccgagccg ttctttcgtc tgcttggcgc ccatcggatc gagtcgctcg
     1501 acgcctcgga tttcgagggg gcgacgaccg tccacgacct taatcagccc atagcctccg
     1561 aactcaaagg gcaattcgat gtcgtgtacg acggcggaac gatcgagcac gtctttaatt
     1621 tcctggtggc gatccgcaac tgcatggaac tggtgagagt gggcggccgg gtgatcctcc
     1681 acacgacagc caacaactac ttcggccacg gcttctacca atacagcccg gaactcttct
     1741 tccgcatctt cacggcggac aacggattca gcgtcgagcg cctcatcgcc atggaatacg
     1801 gcccgcgtcg ccggtggtac gaggtgagcg atccggcgtc gatcaaggcc cgcactcccc
     1861 tgatcaacgc ctgccccgtc ctgctgttcg tgcaggcccg aaaggacgcg cagcgccccc
     1921 ttttcgaaca ggtgccgcaa cagagcgact actccaacat gtggcaggga tgcgccgccc
     1981 ggggcaaatc agggtccgct ccgcacgagt gtgcgccgac ctccgccggc gcgcgattca
     2041 agcgggcatt gatcgagcga gcgcccgcat cggcacggat gctcgaagca ttcaagttct
     2101 cgaactggaa ccgcgccttc tctttccgca atcgcgctgc gttcaagcct gtgaacaagc
     2161 gccgcctttg agggcgccac acgctctcga tcacacccgc gctttttcag tgaaagcatt
     2221 tctcggagcc tcggcctccg gaatgcggaa cgtcagagat cacctgacct ggtgccggta
     2281 gaacagccgg tgctgcaccc tccgcctgaa ccgctggaag ggggtcaacg cccgcccgtc
     2341 gggcggcaag gtctcgagcg cggccctggc ggccaccggg atgagcttcc gcgcggcatc
     2401 gagcgagtag acattgtgat agatgcgaac gccatcgagg tgactcacgg tgggccaccc
     2461 gcgaacccgg gttggagcca ggcaaggccg gtagttgtac tgacacggaa gcaggattag
     2521 gtcgtcatag tagcgcgtgt ggacgacttc gttgagagtg agctggtctt cgatgctccg
     2581 aaaacgggcc cgaccccgcg caacaacgtc cgcgcgaatg cgccgcgacg ccgcaatgtt
     2641 ccagaatgtc accccggcgt tgacgtacga actggtgtat ccctgacccg cgatgtactt
     2701 gcccccagca tgctccacac aggcgccgac cgccgcccca cgcagcagag ccgggatctc
     2761 ggggattggc tggaggcaca ggacatcatg gttgtccacg atcagcgcgt aatcgcaatc
     2821 gagctcatcg atcagcgcaa agcggcggta ataaatctcc cgcagaaagt gctggtacgt
     2881 cgggtccggc tcgcgaaggt ggtagacgat gcccggcaac tgccgctggg gcaggcgttc
     2941 gcccaggatg aaggcgtgca gctcgaggaa aggattctga ccaaaactcg tgaacgttcg
     3001 gtccagaatc tcggtgtgct gcccggaagc cacggtgacg actgcctggc gggacggctt
     3061 catggtttcg cctcgcatct caaagatgcg gagcatgttg cccctccatc agaggatcaa
     3121 gggcaaaagg ccaactgcct gcccccagcc ttgaccggtg aaacatgttg cagacaagtc
     3181 aagtttctgt gctgcgttaa acgggtcgag gattctactg agcgaggcgc gggagagaag
     3241 ggcggggaga cacgggggac tcctcagaac accgacaaac cagatgcacc gcgccttgac
     3301 ccggacggat ctcgcgtcta gactgaggca cggtcagaac cggcagtgag cgagtcgagt
     3361 ttgttcggcc gcctttggcc ccacacaatg caagcggaaa tcgtcatcga gtactgcgtg
     3421 gcctgaaact acgaacccca tgccgtcggt ctggcggcgc aactgctggg tctcaagttc
     3481 aagatcgggt cgctcaagct catcccctct tcgggcggag tcttcgaggt gagtgtgaac
     3541 ggacgcaacg tctactcgaa gaaggccact ggccagttcc ccgatccgga ggcggttatg
     3601 cgcgacgtcc gcgccatgct gaagtaggcg cggcgcgagg cccgggttta acccgcatat
     3661 ttcacagcgc ggcaaagccg caaccaaact ctattctttc aagaaaagag gaagtgcata
     3721 gagagtggac accccgtcag tgcacagggt ggtcagacct cgcatgatgt ctgctatgcg
     3781 ggttagaaac cacggttcac ggaaagcgcc gcctgtgcgc ggcatggcgt gggaggctcc
     3841 cacccgccgg cttcactcgg cgatcgcttc cccgcgcgct tggaaccgat ggtagagacg
     3901 attggccagg gggagaagaa tggcgacggt gagggcgccg gcgacgacat cgaccacgta
     3961 gtgatagcgg caatagacgg tcgaaataca caggagcagc gccacaacca gatgcagcca
     4021 tcggatgcgc ggcaggtagc ggaatgagaa ccagaccgtg cagagtgcga ttgcgacgtg
     4081 gctgctcggg aaggcggcgc cggggctctc gaaattccga taaatccagg ccatgatgtt
     4141 gaagaagacg ccgtgtgtca cggcatccgg atacgccagt gggcccgcgg aagcctgaat
     4201 cgcatcgggc agccggaacc cgccgatccc gcggaagaag gcgcgcggcc ccatgacggg
     4261 caggcagatg taggtgaggt agcaaaggta gaagacaaac gacaccacgg acacatagtg
     4321 gtagaactgt aggcgccggc ggacgaacag ggcgagcccg acgccaacaa tcatgatgta
     4381 ataggagaag taggaggcat agagcacttc gctgactgcg agatacggga aggcgtccat
     4441 caaccagagg cacggctgca gcccgaagag actttcctcg aggcggatga agtggacatc
     4501 caggaagtcg cccgcgaaca tatggttgag catccccgtc tcccgataga atccggtata
     4561 gagcagaacc ggatagaaat gccgcaggaa gtcaaggaac cggttcgacg gccagcgggc
     4621 gtgcgactgg atgaggagat gcaccaggac caggcaggcc gcgtggaccg ccaccaggca
     4681 cggccagcat ggcacggtct ggttgtgaaa cagcagaatc agaacaccta caagtgcgat
     4741 gtacgcctgc gtcgcataat cgatgaaaat gtagtgtttc aagagttcca gtccatccat
     4801 cggtgttcgc gataccaggc cagcgtcgag accgcgcccg cggacaacgg cgtgggacag
     4861 cgcaccccca actcgcgctg caaacggccc gcgtcgcaca cccatcccgg agccaacagc
     4921 tcgcggtgct tgtcgtgcgc gagcaggctg gcccgtcccg agagccgcgc ccccgcggtg
     4981 gcggcggcgc aaaccaggcg caccagcaaa cccggcacgg gcacctgctt cacgcggcac
     5041 cccaatgcgc gcgcgatcaa ttcggtcacc tgtgttcctg tcgccacctc gtcgccggcg
     5101 acgtgaaaga cctttcccgc cgccgcgggg tgatccaggc aagcgatcgc cgccgacgcc
     5161 acatccgcac catacaccag gctcaaaggc tgccgccctc ctgcgatcgc aggcgcccag
     5221 ccgcgttgca cggtccgaaa cagccgcagg aactcgcgat cccgagggcc atacaccgcg
     5281 ggcggacgca gaatggcgta ctcgacgcgg cagcgttccg tcacctcgcg ttccgcggcc
     5341 agcttgctgc gtccgtattc ggaaaccggg tgtggcgtgt cggtctcgcg cgcgggggct
     5401 tgggcggtgc ccgggccgca ggctgccagg ctcgagacga ggataaggcg gcggatttgg
     5461 ccgggtcggg cggcgagggc gtcgacgagg tgggcggtgc caccccggtt gacggcatcg
     5521 aattccgaga ggcgcagcgc cttggtcgcc cccgcgcaat ggatgacatg cgtgatgcca
     5581 gacagggcct cgcgcaggct ggccggatcg gtcacggatc cttggcgcac ctcgacgcga
     5641 ggcagatgat cccggacgaa ctgcagattg ccggttggcc gcgccaggag gaccgcgggg
     5701 atgtcccggg cgcacagcgc atccaggata tgactgccga ggaatccgtt gccgccagtc
     5761 aacagcacgt tcatgagcaa cggctcaaat cacaccgagt tcgcgtccga cccggtccat
     5821 catttcgagg gcgaaatcga tctggttgtc agtgtgggtg gccatgaggc tgatgcgcag
     5881 cagctcctgg cctggcggca cgccgggcga gacgaccgga ttgacgaaca cgccctgctc
     5941 ttcgagctgt ctgcagaact tgaatgtcgt caacagatcc cgcacgtaca cgggcaggat
     6001 cggcgtctgc gacgccccaa gatcaaaacc gagcgccagc aggccctgct tcatgcggtc
     6061 ggtgttgtgc cagagccgtt ggatgcgctc cggctcgccg gtcatgatct tgagggcggc
     6121 gagcacggcg gcgacgttcg ccgggctcat gctggcgctg aagatcagcg gcttcgagtg
     6181 gtgtttgagg taatcgatcg tggccttgtc ggaggcgatg aaaccgccca ggctggcgag
     6241 cgatttgctg aacgttccca tgatgaggtg aacctcatcg accaggccga aatggttcgc
     6301 cgtgcccgcc ccccgatccc cgagcacgcc gatggcatgg gcgtcgtcca ccatgaccac
     6361 cgtcccgtac tccctcgcca gcttgcacaa atcgggaagc tggatgatgt ccccctccat
     6421 gctgaacacg ccgtcgacca caatcagctt gccggtctcg cgctcgatca tctgaagacg
     6481 gttctcgagc gccgccatgt ccttatgggc gaagcggacg aacttgccct ggcacagaag
     6541 gcagccttcg acgatgctcg cgtgatcgct tttgtcggcg agcacatact cacctttgcc
     6601 gaccagcgag ctgatgaccc cgaggttgac ctggaacccg gtgctgtaga gcagcaccgc
     6661 ctccttctgc accagccgcg ccagcgcctc ctcgagttcg aggtggatgt ccaacgtccc
     6721 attgaggaac cgcgacccgg cacagccgcc cccgtacttg tccaccgccg ctttggcggc
     6781 ttccttgatt ttcgggtggt tggtcaatcc caggtagctg ttggatccca gcatcagcac
     6841 cttgcgctcg ccgatggtca cctcggtgtc ttgcgccgag gaaatggttc ggaaatacgg
     6901 atagagaccc aggcggcgca cttcctccgc attgcggaat gcgcgcgttt tctcgagaag
     6961 gcgcggaacg tgcttcgatg ggcttcgggc ggacctggcc tcctgccggc tttcggcggg
     7021 ggcggcgtct ggaacagcgt ccgcatcttc aggcgcgtcg gagtgcgcct gttcttcgag
     7081 ttcctccttg tggtcagtgc gtttcataat gcgatcgaag gcccggcgcc tcacttcgcc
     7141 ccggcaggca ccgggaatcc ccgatcgcgc atgagcgcgg cgatcccggc atcgcgcccg
     7201 cggaactgcc ggtaagcctc ggtcggctcg accgtgttgc ccgcggagag cacgtggcgt
     7261 ttgaatcgcg cggcgatgga cttgtcgtag aagccgccgg cttcgaggaa cgcctccgcc
     7321 gcatcggcgg tcagcatgtc ggcccagagg tagctgtagt agccggcgga gtagccttcg
     7381 ccggcaaaga tatgggcgaa gtgcggcatg cgatggcgca tgacgatctc ggaaggcatg
     7441 ccgagtcgcg ccagttcatc gcgctcgaat gccgccgggt cgatcgcccg ctccccggcc
     7501 aaatgcagtc tcatgtcgat caggccgctg gccaggaact cgattgtggc aaacccttgg
     7561 ttgaacttcg aagccttctg gatgcgctcg gccagcgcgg ccggcatggg ctctccggtc
     7621 ttcgagtgga gcgcgaagcg gttgagcacc tcgggcgtcg ccagccagcg ctcgttgatc
     7681 tgcgacggca gctcgacgta atcgcgcggc acgcgcgtgc ccgacagcga cgggtaggtc
     7741 acgtccgaac acaggccgtg cagcccatgg ccgaactcgt ggaaaagcgt cttggcatcc
     7801 tcccaggaca ccagaaccgg ctcgcccgcg cctcctttca tgaagttcga gttgttcgag
     7861 accagtgtcg tgatcgggcg ctcgaaccgt tcctggttcc gataggcgtt catccaggcc
     7921 cccgaccgct tgcccgttcg cgcatacgga tcgaaataga acaacccgac gtggcgcccg
     7981 tcggcgcctc gaacctccca gacacgaacg tcgccatgat acaccgggat gtcggccgcc
     8041 ggcgtgaagg tcagcccata aatctggccg gccgcccaga acatcgcctc gcggagcttg
     8101 tcgagctgca ggtagggttt cacctcgttc tcgtcgaggt cgtatttcgc cttgcggacc
     8161 ttctcggcgt agaagcggta gtcccaaggc gcgatccgga ttcccgcgcc ctcggcgtcg
     8221 gcgatcgcct gcatgtcggc gacctcctcg cgcactcgcg ccagggccgg tttccagacc
     8281 gcttccatca attccatcgc ccgctcgggg gtccgagcca tcgagttctc cagccgccag
     8341 tgggcgtggg ttgcatagcc caggagccgt gcccgctcgg cccggagccc taggatttct
     8401 gggatcaacg ccttgttgtc gtgggcgccg gggcgatcgc cgcggctaac gaagttgtgc
     8461 cagacctgct cgcgcaagtc gcggcgggtc gagtaggtca ggaacggttc gacgctcgat
     8521 cgggtgttcg caatcaccca ttggcccttc tgcccccgtc cctccgcctc ggcagccgcc
     8581 gcggcccgaa acgactccgg cagcccggcc agcccggcgg catccgcgat caccgtggcg
     8641 tacccttcct cgtcggcgag cagattctga ctgaacgttg tgtagagggt cgcaaggcgc
     8701 tggttgatgg tcgccacgcg cgccttgccg gcggcgtcga gccgcgcgcc ggcccggacg
     8761 aactccgtgt agtgcagcca gaccaggcgc tgctgttcgg acgtcagccg ggccttgtcc
     8821 ggcgactggt agatggcctc gatgcgcttg aagagattct cgttttgaaa gacctcgtcg
     8881 cgcaacgccg ccagcttcgg ctccatctcc tgctcgatgg cccgaaactc cggcgtgctc
     8941 atcgtcgagc cccagacgcc gtaaacagcc tcgacccgat ccagggcgcg ccccgccttc
     9001 tcgagcgccg caatcgtgtt gtcgaaattg gccggagcgg actggtcggc aatctgccga
     9061 atctcagccc ggtaggcccc gatcgcggtc tcgaaggccg gtttgaaatg cgccacctcg
     9121 acccggtcga acggcggcac gcccccgtac gggcctttcc actcggacgc cagcggcccg
     9181 gcgtccggca cctcggacgc ggccaggccg gaggcgaggc tgagcgcgcc gacacccgtc
     9241 gcgagaacgc ggctcataga acgaaaaaaa ccggcgcgca atgcgcccct cgaatagtca
     9301 tgatcacaat gcacaagtca gatttaacga acgttcaacg ttcaacgctc aacgctcaaa
     9361 gaaacgtctt gttgagtgtg ttgggtgtcg gttccctgcc cggcatgaca cccgtcgaca
     9421 aacgggccgt atggtaaagg ctcgaccggc ggagtcaaat cctggtgttg caccgtggtg
     9481 gaccaatgga ccccatcgcc gcccacggtg ttcgggaatg acaggaattc accacgaaga
     9541 accgaagaac tcgacgcggc agggccgccg ccaagactcg cagggccgat cgccgctccg
     9601 acaaaacgac ctttctcaaa caactggcca tccgagtcgc caccgggcaa cacgctgaca
     9661 agcgatttcc cacgttgctc ccactctcgg cgtacgccag cgccttcgcg gaacaaaacc
     9721 tctcgttcac gatcgtggcc ttgaaaggcc aatccgcggc catgaatcac acgtgacgcg
     9781 aatccaagaa tcgcggagaa ccacgacaac ttcgaccgcg gagtaggaga atggacggat
     9841 gtcagttcgc tgcgcgcccc gcgatgccct tgatgccttt ggcgatggtg cgggcgggtt
     9901 ccttgggccg gagagcgcgg acggcggcgc cggccagcca catctcggaa tcacgaatcg
     9961 cgcgcagttc cttttggagt tgggcctggt aattgggggt tccgcacgcc ttgagcacac
    10021 gggcgcattc cttgccggtc ttgacgcgct gatacagctc cttgaacacc ggcaggacgg
    10081 ccttcttgaa ccgcggcttc caatcgagcg caccgcgctg ggcggtggcc gaggtgttcg
    10141 tgtacatcca gtccatgccg ttttcgtcga ccagtcggat gaggctctgg gtcagttcct
    10201 cgaccgtttc gttgaaggcc tcgctcggcg tgtggccgtt ggcgcgcagc acatcgtact
    10261 gggcttccat gatgccggcc agggcgccca tgagtacgcc gcgctcgccg gtcaggtcgc
    10321 tgaagacctc gtgttcgaac gtggtgggga agaggtagcc cgatccgatc ccgatcccga
    10381 tcgccagaca gcgctcctcg gcgcgtccgg tggcgtcctg ctcgacggcg aagctcgagt
    10441 tgatgccggc gccggtcagg aagttgcggc gcaccgaggt gccggacccc ttcggggcga
    10501 ccatgatgac gtcgacgtcg gcgggaggca cgacgccggt ctgcttcctg taagcgatcg
    10561 agaatccgtg ggagaaatag agggcgtcgc ccggtttgag gttggccttg atgacgggcc
    10621 agacggcgcg ctgggcggca tcggagacca gaatctggac gatcgtagcc cggcggaccg
    10681 cctcgtcgat ctcgaagagc gtcttgcccg gtttccatcc gtcctggacg gcgcggtccc
    10741 agtcgcgctt gaactggctg gcctggccga cgatgacgtt gaagccgttg tcgcgcaggt
    10801 tgagcgattg ggccgggccc tggacgccgt acccgatgat ggcgatgacc tcgttcttga
    10861 ggaccttgcg cgccttggcc atcgggaatt ccttgcgcgt gatgacctgt tcccgtgtgc
    10921 cgccgaaatc aatagttgcc atgctcgata tgccgtgatg gtggtgtggc at
//
LOCUS       DAOQRO010000077        10969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5212_length_10969_cov_5.225948, whole genome shotgun sequence.
ACCESSION   DAOQRO010000077 DAOQRO010000000
VERSION     DAOQRO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5212_length_10969_cov_5.225948"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3332
                     /locus_tag="PKK20_04450"
     CDS             <1..3332
                     /locus_tag="PKK20_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99168.1"
                     /translation="SVWWKGRAVVDNLAIDFNPSAGRLVFGGRTGDAYQNTHVDDIVL
                     TTIPADKALIGNATADAVSVTVLLEDAGSSIVNESSVTMKVNGAAATPLTFSKDGPTT
                     TVRWASPTYLASGSTVTVDVEFKDSSDRTITGTRTATVAAYARVPAAYQVPTASVDTA
                     KPGFLLRPYQTEEAQPNSLIWTEGQLLGWYGPNIADLTGATGGFFTHEGVINFNMDAP
                     NNAGNFTGDTMFPGFPGTTFSTGNATEEVLTYIEFPTAGMYQLGVNSDDGFRVTAGRA
                     PGDLLGVILGYFDGGRGASDTVFTIVVDPPGIYPIRLIWENGSGELPGNGANLEFFSV
                     KDGVKTLINDVANNGLKAYRESTAAGPSVSDMTPLRGATGVAGNATVMAVISHGSVTI
                     GDATVVAKLNGVVTPASVTTAAGKTTVTVQPPGGLLPSGATVTATIEFSDTATPAKAY
                     STSWSFTVATYTELSDCLWTAPGTVDTTKPGMKARVWQLDQVGSNTIVNRIHRAEQQL
                     AGVIGPNVADLSLAVDGWFHCEQINWDADSADNGNWPFSYYGTTVPGIPGATTGGFPT
                     DNIAAEIVTYIDFPTAGFYGMGVNSDDGFKVTVADTPPKYNLALVISGAASAAGSYHA
                     ISAPEATAKQWTAGVSAKLVYMDPHEGCTAPVNAAELAGNIALVDRGTCEFSAKIMAA
                     KNAGAIACIIVNNRPVGHAEGIWPIAVGVGTAGYQDLPTVMISMPDGDKIKTGLASGL
                     TATIIPDITPPLGEFDDGRGAANTDFFFIVPKAGVYPFRCVWFEGNGGANCEWYSITP
                     SGQRILLNDPENPAALKTYWARTAQPEPCKKTYSIGLNFGADEVNGSNSGTLAAKDVA
                     GVPSVAQANWNNLRLAGGNGSVMADAYGVREATAMTVVWDSANTWSSTGRGEENNAFT
                     GADKVLMTGYLDTGNATTSTVKITGIPDELSSQGYDVYVYALGGVEARGGGYRVVPEG
                     GAATAYVDVQAPMDPTTYVQAIPTPGSWAVGNYIVFGGMTASSITVEATTEGGHGFGG
                     TPRAPINAIQLIAPTTVVPVPAITIAKNADGTITISWTNGGTLQAATSILGPWQDVTD
                     TSPYTFTPEAGVPHMFGRIKK"
     gene            3513..6005
                     /locus_tag="PKK20_04455"
     CDS             3513..6005
                     /locus_tag="PKK20_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNU99169.1"
                     /translation="MKTVHRFGLSRPASSSPTPTARVFVSQTRHEISDPVNDRRVLVL
                     GLLSAFILVIPSGCRTDRPEAKVQSAKLAAAFDASKLAEMDAAIEAAIADKRCPGGVL
                     WLERNGVAYRKAYGLRAVFPSEEPMTEDTLFDAASLTKVMACTPAVMRLVEKGRLQLD
                     APVSNYLPEFKGNGKEAITVRQLLTHTSGLRPDVDLKPDWSGTEAAIALACAEKLRAR
                     PGEEFIYSDTGPILLGEVVRRVTGQPLDQFLAREIYGPLRMKDTGFNPPQAKLPRIAP
                     TEIENGRPVRGVVHDPRARRMGGVAGHAGLFTTASDVARYSRMMLNEGKLDGVRVFRP
                     ETVRLMTSVQTPPHVTARRGLGWDLDSAYAGPRGAWFPAGSYGHTGWTGTSLWIDPFS
                     RTFVIFLSNRNHPDESGSVTALRRKLGTLAAEAVRGYNFVYVPGALPALTNAPAVRAS
                     NAGTNRAVLNGVDVLVRQGFKPLQGLRVGLISNHTGHDRQRNATIDLLRSASGISLKV
                     LFSPEHGIRGQLDEKVDDSVDAQTGLPIYSLYGEVRQPKPEQLRDLDALVFDIQDIGC
                     RFYTYISTMGLCMEAAAKAGLKFFVLDRVNPIGGIAVDGSVHSGSSTFTAFHPIPVRH
                     GMTVGELARMFNAEREFKCDLMVIPVAGWSRAQWFDQTGLPWTNPSPNMRSLTEAALY
                     PGIGLLETTALSVGRGTGTPFEIVGAPYIDDVKLAEELTRAALPGVRFVPVRFTPNAS
                     VFKDKACAGVQIFLTDRDRCEVIDVGITIAQTLHRLYPQDFAIAKFNNLLQHPPTIEA
                     IRAGKSLRDIRRLWLADLAAFKQRRSGFLMYE"
     gene            complement(6012..6782)
                     /locus_tag="PKK20_04460"
     CDS             complement(6012..6782)
                     /locus_tag="PKK20_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNU99170.1"
                     /translation="MRLLEGKTGIVFGVANRRSIAWAIAQAWAQAGATLAFTYQGERL
                     KENVEELAATFGADTLILPCDVRQDDEIASVFAALENKFGTLHLLLHSVAFAPRDALE
                     GAFINTSREAFRIAHDVSAYSLVALARAAAPLMTEGGSILGLSYYGARKVVPHYNVMG
                     VAKAALEASTRYLAYDLGPKRIRVNCISAGPVNTLAARGIAGFQDMLRHYELKAPLKR
                     NVTADELGATGTFLASDGAAAITGQVLYVDCGYQIMGM"
     gene            7121..7903
                     /locus_tag="PKK20_04465"
     CDS             7121..7903
                     /locus_tag="PKK20_04465"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99171.1"
                     /translation="MRNGLARRIVSLVALSVALEAQPLPGTSPESPANRLTPAERAAG
                     WRLLFDGTNTSGWRGFKKPAFPDKGWIIEEGCLKHERRGGGGNIITDRTFNDFEFSFE
                     WRLSPRANSGVKYFITERRAGPIGHEYQIIDDKAHGFARPGRKQQTAGFYDVLAPTAW
                     QEPRPPGQFNHSRIVVQGSNVQHWLNGLKTLEYELGSPQVLEAVAASKFKEIEGFGVK
                     LEGHLLLQDHGGEVWFRNLKLRELPAQGPVSPVTTSRGSAAQ"
     gene            complement(7975..9399)
                     /locus_tag="PKK20_04470"
     CDS             complement(7975..9399)
                     /locus_tag="PKK20_04470"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNU99172.1"
                     /translation="MLVIIVAAALHSGATAALADADIGPARAPSAVHIQESNAIAPAW
                     RLPPIRPVTRVNPRLANYFHIDLAEGRVDHKERRLAQWNVVILNHDIVLKEKLSLARM
                     RQTNPRIKLLAWIPLQGPNDGLAKGVPAPGDEDWYARRADGTYLTPHWGGRLMDVCVQ
                     SRAWLRHLLRYVRRHCLGAGGYDGLMLDCLRPAEPPGHDANRDGVHDARDTAAWRESM
                     LFLLRRLRAEYPGAILVGNGGLPWPADCPYYEYANGCMHENALGDQFGGTEWRVLWNA
                     CRTALSRISNRPAYHLIQADIRADRRTQAVAADQRALTPNDCRRFRLGLATALLVDGG
                     YFGFDRGDCLHGQLWWFDDYDCDLGAPLGASIEGRYGPGTFARDFERGLVVVNPTQTP
                     VWVAHTPDSSGGIGGARASAFTVPPRDARILPAGLRKPAASPGRSSRTGRPEQAAEPA
                     PARLDPRPSAGFLPERSCAQNSGQ"
     gene            complement(9487..10821)
                     /locus_tag="PKK20_04475"
     CDS             complement(9487..10821)
                     /locus_tag="PKK20_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99173.1"
                     /translation="MKSRIHTDRCLLSCALAALSLAALPVDAWEVATNLTVKTELSAK
                     EAYDSNVYLQDHKPDTDAVPRAVRPDQDSFVTSVTPKFSLDWKPCSAFTAAVSYAPEV
                     TFYHAESSEDYVAHRGAINLRGEVGDVPWEMPHSITFIDGNRKGLYYGVSSLPGQLDG
                     VPAIGGIPVRDRREQFVYRGGFKTTWSLGAFFIRPVFAAYVHDFQTKQKRNGRLLPDG
                     APNPDWGYENYVDRSALNYGLDMGWSVQARTKLYAGYRYGNEFEGTMVGSLYRYDAQV
                     HRPLVGIEGKPVSWLTADVSIGPDIHNTTSRHDPAFDPDYTALWVDAVVTLTPTAKDS
                     IALIWRQNTQPAFSSPSIYEDTVYEGTVRHRFDNQWSVGGGFRAYLGDWHDPVIRRDW
                     IFTPSASVTYRHDAHLSAEASYSYDWVDSRVPNTKGREFTRHLAWLAVKYAF"
BASE COUNT         1989 a         3583 c         3529 g         1868 t
ORIGIN      
        1 tgtcggtgtg gtggaagggc cgcgcggtcg tggacaacct ggcgattgat ttcaacccga
       61 gcgcagggcg gctggtcttc ggcggtcgca cgggcgatgc ctaccagaac acgcatgtcg
      121 acgatattgt cctgaccacc atccctgccg acaaggctct gatcggcaac gcgactgccg
      181 acgccgtcag cgtcacggtt ctgctcgaag atgcgggctc gagcattgtg aacgagagca
      241 gcgtgacaat gaaggtgaat ggcgccgccg cgacgccgct gaccttcagc aaagacggcc
      301 ccacgactac ggtccgatgg gcctcgccga cctacctggc ctcgggctcg actgtgaccg
      361 ttgacgtcga gttcaaggac agttcggatc ggaccatcac gggcacacgg acggcgacgg
      421 tcgctgcgta cgcccgggtc ccggccgcct accaggtgcc gaccgcatcg gtcgacaccg
      481 ccaagccggg ctttctgctc cgtccgtatc agactgaaga ggcacaaccc aattcgctga
      541 tctggactga gggccagttg ctcggctggt acggccccaa catcgccgat ctgaccggcg
      601 cgacgggcgg gttcttcacg cacgaagggg tcatcaattt caacatggat gccccgaaca
      661 atgcagggaa cttcaccggt gacaccatgt tccccggttt ccccgggacc acctttagca
      721 ccggcaacgc caccgaagag gtactaacct atatcgagtt cccgactgcc ggcatgtatc
      781 agctcggcgt caacagcgac gacggttttc gcgtgactgc cgggcgcgcc ccgggcgatc
      841 tgctcggcgt catcctcggc tacttcgacg gggggcgcgg ggcttcggac acggtcttca
      901 cgatcgtggt cgatccgccc ggcatctacc cgatccgcct gatttgggaa aacggctccg
      961 gggagcttcc cggcaacggg gccaacctcg agttcttcag cgtcaaggac ggcgtcaaga
     1021 ccctgatcaa cgacgttgcg aacaacggcc tcaaggccta tcgcgaaagc acggccgccg
     1081 gtccttcggt gtccgacatg accccgctgc ggggcgcgac gggcgtggcc ggcaacgcga
     1141 ccgtcatggc cgtgatcagc catggatcgg tcacgatcgg cgatgccacc gtggtggcga
     1201 agctcaacgg tgtggtgact ccggcttccg taacgaccgc ggctggaaag acgaccgtca
     1261 ccgttcagcc tccgggcggc ctgctgccct cgggcgcgac cgtgactgcg accatcgaat
     1321 tcagtgacac cgcgacgccg gcgaaggcgt acagcacatc gtggtcgttc acagtcgcca
     1381 cctacacgga gctcagcgac tgcctctgga cagcgcccgg aacggtcgac acgaccaagc
     1441 cgggtatgaa ggcgcgcgtg tggcagttgg accaggtggg ctcgaacacg atcgtcaacc
     1501 gcatccaccg cgccgaacag caattggccg gcgtcatcgg gccgaacgtc gccgatctca
     1561 gcctggccgt cgacggctgg ttccactgcg aacagatcaa ctgggacgca gacagcgccg
     1621 ataacggcaa ttggccattc agctattacg gaacgaccgt cccaggcatc ccgggcgcga
     1681 cgaccggcgg gttccccacc gacaacatcg ccgccgagat cgtcacctac atcgacttcc
     1741 caacggccgg gttctacggc atgggcgtca acagcgacga cggctttaaa gtcaccgtgg
     1801 ccgacacgcc gccgaaatac aacctcgcgc tcgtgatcag tggtgccgcg tccgccgctg
     1861 gaagctacca cgccatctcg gccccggagg ccacggccaa acagtggacg gccggcgtga
     1921 gcgcgaaact ggtctacatg gatccgcatg aaggttgcac cgcgcccgtc aacgcggcgg
     1981 agttggccgg caacatcgcc ctggtcgacc gcgggacgtg cgagttctcc gccaagatca
     2041 tggcggccaa gaacgcaggc gccatcgcgt gcatcatcgt gaacaaccgt cctgtcggtc
     2101 atgccgaggg catttggccg attgcagtgg gtgtcggcac agccggctac caggacctcc
     2161 cgacggtcat gatctcgatg cccgacggcg acaagatcaa gaccggtctt gccagcgggt
     2221 tgaccgctac gatcatcccc gacatcacac cgccgctcgg ggagtttgac gatggccgtg
     2281 gagcagcgaa tacggacttc ttcttcatcg tgccgaaggc gggtgtgtat ccgttccggt
     2341 gcgtgtggtt tgagggcaac ggcggggcca actgcgagtg gtacagcatc actccatccg
     2401 gccagagaat cctgctcaac gacccggaga atcccgctgc cttgaagacc tattgggctc
     2461 gcacggccca gcctgagccg tgcaagaaaa cctatagcat cggtctgaac ttcggtgcgg
     2521 acgaggtcaa cgggagcaac agcggcacct tggccgcgaa ggatgtggcg ggtgtgccgt
     2581 ccgttgccca ggccaactgg aacaacctga gactggccgg cggcaacggc tcggtgatgg
     2641 cggatgccta cggcgtccgg gaagcgacgg ccatgaccgt cgtctgggat tccgccaaca
     2701 cgtggtcctc gacaggccga ggcgaagaga acaacgcctt caccggggcc gacaaggtcc
     2761 taatgaccgg ctatctcgac actggcaacg ccacgacctc gaccgtcaag atcaccggca
     2821 ttcccgatga actcagcagc cagggctatg acgtctacgt ctatgccctg ggcggcgtcg
     2881 aggcccgcgg tggcggttat cgtgtggtgc cggaaggcgg cgccgcgacc gcttacgtgg
     2941 acgtccaagc tccgatggac ccgacgacct acgtccaggc tatcccgact ccggggtcct
     3001 gggccgtggg caactacatc gtgttcggcg gcatgaccgc gtcgtccatc accgtcgagg
     3061 ccaccacgga gggtgggcac ggattcggtg gcacgccgcg ggccccgatc aacgcgatcc
     3121 aactgatcgc gccgaccacg gttgtgcccg tgccggcgat cacaatcgcc aagaacgccg
     3181 acggcacgat caccatctcg tggaccaacg gcgggacact ccaggccgcg accagcatcc
     3241 tcggaccgtg gcaggacgtg accgacacga gcccgtatac gttcacgccg gaggctggcg
     3301 tgccgcacat gttcggccgg atcaagaagt aggattcact gcgtttccac gcagccgcac
     3361 agagaccggg cttcggcccg gtctcttttt ttgtcgcttg aacgctgaat atgtcaccga
     3421 actaatcgcc cacccgggcg cgcagcgtag acatgccgcc ccggtcccaa tctccgcttg
     3481 gcgcgcaggg caggcttgct tagcctgcac ccatgaagac cgttcatcgt ttcgggctgt
     3541 ctcgccccgc ctcatcgagc ccgaccccca cggcccgagt atttgtctcc cagacccggc
     3601 atgagatttc cgatccagtc aacgaccgcc gcgtccttgt ccttgggctt ctgagcgcgt
     3661 tcatcctggt cattccgtcc ggctgccgca ccgaccggcc cgaggcgaag gtgcagtctg
     3721 cgaagctggc cgcggcgttc gacgcctcca aactcgcgga gatggacgcg gcgattgaag
     3781 cggcgatcgc cgacaagcgg tgtccgggcg gggtgctgtg gctcgagcgc aacggtgtgg
     3841 cctaccgcaa agcctacggg cttcgcgccg tgttcccttc ggaggaaccg atgaccgagg
     3901 acaccctgtt cgatgccgct tcgctgacga aggtgatggc ctgcacgccg gccgtcatgc
     3961 gtcttgtgga gaagggccgg ctgcaactcg atgcgcctgt ctcgaactac ctgcccgagt
     4021 tcaagggcaa cggcaaagag gccatcaccg tccgccaact cctcacccac acctcgggtt
     4081 tgcgtccgga cgtcgacctc aagcccgact ggagcggcac ggaagcggcc attgcgcttg
     4141 cgtgcgccga gaagctgcgc gcgcgtccgg gcgaggagtt catctacagc gacacgggac
     4201 cgatcctcct gggcgaggtg gtgcgccgcg tcaccggcca gccgctcgat cagtttctgg
     4261 cgcgggagat atacggcccg ctgcgaatga aggacaccgg gttcaatccg ccccaggcca
     4321 agctgccgcg catcgccccg acggagatcg agaatggccg gccggtgcgc ggcgtggtgc
     4381 acgacccgcg cgcgcggcgg atgggcggtg tggcgggcca cgccggactg ttcaccaccg
     4441 cgtccgatgt cgcccggtac agtcgcatga tgttgaacga gggcaaactc gacggggtcc
     4501 gcgtgtttcg gcccgaaacg gtgcggctga tgacttcggt gcaaacgccg ccccacgtga
     4561 ccgcccgccg cggcctgggc tgggacctcg attcggccta cgccggaccg cggggcgcgt
     4621 ggttccccgc cggcagctac ggccatacgg ggtggaccgg cacatccctc tggatcgacc
     4681 ctttctcgcg cacgtttgtc atcttcctgt ccaaccggaa ccacccggac gagagcggca
     4741 gcgtgacggc gttgcgccgg aagctgggta cgctggcggc agaagcggtg cgcggctaca
     4801 acttcgtcta cgttcccggc gccctcccgg cactcacgaa cgccccggct gtcagggcct
     4861 cgaacgccgg cacgaatcgc gcggtgctca acggcgtgga tgttctggtc cggcagggat
     4921 tcaaaccgtt gcagggcctg cgcgtggggc tgatcagcaa ccacaccgga cacgaccggc
     4981 aacgcaatgc cacaatcgat ctgctgcgaa gcgcgtcggg catctcgctg aaagtcctgt
     5041 tcagccccga gcacggcatc cgcggccagc ttgacgagaa ggtggacgac agcgtcgacg
     5101 cgcaaaccgg gctgccgatc tacagcctgt acggcgaggt ccgccaaccc aagcccgagc
     5161 agttgcgcga cctcgatgcc ctcgtgttcg atatccagga catcggctgc cgtttctaca
     5221 cgtacatctc gaccatgggc ctctgcatgg aagcggcggc caaagccggc ctcaagttct
     5281 tcgttctcga ccgcgtcaat cccatcggcg gcatcgccgt cgacggctct gtccacagcg
     5341 gctcatcgac cttcaccgca ttccatccta tcccggtccg ccacggcatg acggttggcg
     5401 agctggcccg gatgttcaat gccgagcgcg agttcaaatg cgacctgatg gtgatcccgg
     5461 tcgcgggctg gtcgcgcgcc cagtggttcg atcagaccgg cctcccctgg accaatccgt
     5521 ccccgaacat gcgcagcctc accgaggcgg cgttgtatcc cggaatcggt ctgctcgaaa
     5581 ccacagccct ctcagtcgga cgcggcacgg gcacgccgtt cgagatcgtc ggcgccccgt
     5641 acattgacga cgtcaagctg gccgaggaac tgactcgcgc cgccctgccg ggtgtgcggt
     5701 ttgttcccgt gcggttcacc cccaacgcca gtgtgttcaa ggacaaggcc tgcgcggggg
     5761 tgcaaatctt cctgacggac cgtgaccgct gcgaggtgat cgacgtggga atcacgatcg
     5821 cgcagacgct gcaccggctc tacccgcagg atttcgcgat cgcgaagttc aacaatctgc
     5881 tccagcaccc gcccaccatc gaggcgatcc gcgccggcaa atcgcttcgg gacatccgcc
     5941 gactctggct tgcggatctc gccgcgttca aacagcggcg cagcggcttc ctgatgtacg
     6001 agtgaggtcc gctacattcc catgatctgg tagccgcaat cgacgtacag gacctggccc
     6061 gtgatcgcgg ccgcgccgtc gctggccagg aatgtgccgg tcgcccccag ttcatcggcg
     6121 gtgacgttgc gcttgagcgg cgccttcaac tcgtaatgcc ggagcatgtc ctggaagccc
     6181 gcgatgcccc gggcggccag ggtgttgacg gggccggcgc tgatgcaatt gacgcggatg
     6241 cgtttgggac ccaggtcgta ggccagatag cgggtcgagg cttcgagggc ggccttcgcg
     6301 acccccatga cgttgtagtg gggcacgacc tttcgggcgc catagtagct caatccaaga
     6361 atgctgccgc cctccgtcat caggggtgcg gccgcgcggg ccagggcgac cagcgaatag
     6421 gcgctgacgt cgtgcgcgat gcgaaaagcc tcccgactgg tgttgatgaa tgcgccttcg
     6481 agcgcgtctc gaggggcgaa cgccacggag tgcagcagca aatgcagtgt gccgaacttg
     6541 ttttcgagcg cggcaaacac cgacgcgatc tcgtcgtcct gccggacgtc gcacgggagg
     6601 atgagcgtat ctgctccgaa cgtggcggcg agttcctcga cgttctcctt gagtcgttcg
     6661 ccctggtagg tgaaggcgag cgtggcgccg gcctgggccc aggcctgggc gatcgcccag
     6721 gcgatcgaac gtctgttggc cacaccgaac acgatccccg tctttccttc gagcagtcgc
     6781 atgctgtcgg ctccgttgtt caagccgata gcgttaggcg cgccccgccg ctctggcaag
     6841 ggcggaaatg acagccgttg agagcgcagc gcatctgtag gttgtcggtg ggaatggagc
     6901 ttgcgtcgcg tggggtctgc ttgccgcgcc tgcctgtgcc gtgcccgaca cggcagacag
     6961 gccgctgtgc tttgcggcgc aggcagggcg cgcggcctac ggcgggagtc tccgttcgtt
     7021 ggaggccctg tgccctcacc gggcgaaatg acaccgagaa caaccagatg gatgcgccgc
     7081 gcttgaccag cacgcgggcg cacgctagac aggccgtgtg atgcgtaatg gcctcgctcg
     7141 aaggattgtg tccctcgtcg cactctctgt cgccctcgag gcgcagccgc tcccgggaac
     7201 ctcgcccgaa tcgccggcca accggttgac gcccgccgag cgcgcggcgg ggtggcgcct
     7261 cctgttcgat ggcacgaaca cgagcggctg gcgggggttc aagaaaccgg cgttccccga
     7321 caaaggatgg atcatcgaag agggctgtct aaagcacgag cgccgcggcg gtggcgggaa
     7381 catcatcacc gaccggactt tcaacgactt cgagttctcg ttcgagtggc ggctgagtcc
     7441 cagggccaac agcggagtca agtatttcat caccgagcgg cgcgcgggcc ccatcggcca
     7501 cgaataccag atcattgacg acaaggccca cggctttgcc agaccgggcc gcaaacagca
     7561 gacggcggga ttctatgacg tgttggcgcc caccgcctgg caggaaccgc gtccgcccgg
     7621 ccagttcaat cactcgcgca tcgtggtgca aggctcgaat gtccagcact ggttgaacgg
     7681 gctcaagaca ctcgaatacg agctgggctc gccgcaggtc ctcgaggccg tggccgccag
     7741 caagttcaag gaaatcgagg ggtttggcgt caaactcgaa ggccacctcc tcctacagga
     7801 tcacggcggc gaagtctggt tccgcaacct gaagctgcgc gaactgccag cccaaggccc
     7861 cgtttcgcca gtcacaacgt cccgcggctc ggcggcgcaa tgatcagtcc gatcatcggc
     7921 gccgagagcc tgagcgttct gggctgcgat agcaaacagg ttcaggtctg gaactcactg
     7981 gccggagttc tgcgcacatg accgctcagg caggaaaccc gcggatggac gcgggtcgag
     8041 ccgggctggg gcgggctccg ccgcctgttc cggtcgcccc gtgcgcgaag agcggccagg
     8101 ggaggccgcg ggcttccgga ggcctgcggg cagaatccgg gcgtcgcgcg gcggcacggt
     8161 gaacgccgac gccctcgcgc cgccaatccc ccccgaggaa tcgggagtgt gcgcgaccca
     8221 gacgggcgtc tgtgtggggt taaccacaac cagaccgcgc tcgaagtcgc gcgcaaaggt
     8281 gccggggccg tatcgcccct cgatcgatgc gcccagcggc gcccccaggt cgcaatcgta
     8341 gtcgtcgaac caccagagct ggccgtgaag acagtcgccg cggtcgaatc cgaaataccc
     8401 gccgtcgacc agcagcgccg ttgctagacc gagccggaat cggcggcaat cgttcggtgt
     8461 aagcgcgcgc tggtccgcgg ctacagcctg ggtccgtcga tccgcccgga tgtcggcctg
     8521 gatcaaatga taggcgggcc gattcgagat tctggaaagg gcggtccggc aggcattcca
     8581 aaggacccgc cattccgtgc cgccgaactg atccccaagc gcgttctcgt gcatgcagcc
     8641 gttggcgtac tcgtagtagg gacaatccgc aggccaggga aggccgccat tgcccacgag
     8701 gatggcgcca gggtactcgg cccgaagccg ccggagcagg aacagcatgc tctctcgcca
     8761 ggccgcggtg tcgcgcgcat cgtggacacc gtcgcggttg gcgtcgtgtc ctggaggttc
     8821 cgccggcctg agacagtcca acatcagacc gtcgtaacca ccggcgccga gacagtgacg
     8881 tcggacatac cgcagcaggt gcctaagcca ggcccggctc tggacgcaga catccatcag
     8941 gcgcccaccc cagtggggcg tcagatacgt cccatcggct cgccgcgcat accagtcctc
     9001 gtcccccggg gcaggcacgc ccttggccaa cccgtcgttt gggccctgga gcggaatcca
     9061 ggcaaggagt ttgatgcgcg gattggtctg acgcatacgg gcgagtgaca gcttttcctt
     9121 gaggacaatg tcatggttga ggatgacgac attccattgt gccaggcgcc gttccttgtg
     9181 atcgacccgg ccctcggcga ggtctatgtg aaagtaattc gccaggcgag gattgacccg
     9241 tgttaccgga cggatcggag gcagccgcca ggccggcgcg atcgcgttgg attcctggat
     9301 gtgaactgcg ctcggcgcgc gtgcagggcc gatgtccgca tccgcgagcg cggcggttgc
     9361 gcccgagtgg agggccgcgg ccacgattat gacgagcacg acaatcaacg gtttcttctc
     9421 caatcagggc gctttgtcga cagagctcgg acgctcgtgc acccgtcccg acgcgaggtt
     9481 ttcgggttag aaagcgtact tcaccgccag ccaggcgagg tgccgggtga attcgcggcc
     9541 tttggtgttt ggcacacggc tgtccaccca atcgtaactg tagcttgcct cggcgctgag
     9601 atgggcgtcg tgcctatagg tcaccgacgc gctgggcgtg aagatccaat cgcgtcggat
     9661 gacgggatcg tgccaatcgc cgaggtaggc gcggaacccg ccacccacgg accattgatt
     9721 atcgaagcga tggcgcaccg tgccttcgta cacggtatcc tcgtagatcg acgggctcga
     9781 aaaggcgggt tgcgtgttct gtctccagat cagcgcgatg ctgtccttcg cagtcggcgt
     9841 cagcgtgacc acggcgtcca cccagagggc cgtgtaatcg ggatcgaacg ccggatcgtg
     9901 gcgcgaggtg gtgttgtgaa tatcagggcc gatgctgaca tccgccgtga gccacgaaac
     9961 gggcttgccc tcgatcccga cgagcggccg atgcacctgg gcatcgtagc ggtagagact
    10021 gccaaccatc gtcccctcga actcgttgcc gtaacgatag ccggcataga gcttcgtccg
    10081 cgcctggacc gaccagccca tatccaaccc atagttcaat gcgctccggt ccacgtagtt
    10141 ctcataaccc caatcgggat tgggcgcgcc gtcgggcagg agtctgccgt tccgtttctg
    10201 tttggtttga aaatcgtgga cataggcggc gaagaccggg cgaatgaaga aggcgccgag
    10261 cgaccaggtg gttttaaacc cgcctcggta cacgaactgc tcgcgccgat cccgcacggg
    10321 aatgccgcca atcgctggca cgccatccag ttgacccggc aggctcgaca cgccgtagta
    10381 gagccctttg cgattgccgt cgatgaaggt gatgctgtgc ggcatttccc agggcacgtc
    10441 tccgacctcg ccccgcaggt tgatcgcgcc acggtgggcc acgtaatcct ccgacgactc
    10501 ggcgtggtag aaggtcactt ccggcgcgta actgactgcc gcagtgaagg ccgagcacgg
    10561 cttccaatca agcgagaatt tcggcgtcac cgaggtgacg aacgagtcct ggtccggccg
    10621 caccgcccga ggcacggcgt cggtgtccgg tttgtgatcc tggagataga cattgctgtc
    10681 gtaagcctct ttggcgctca actccgtctt tacggtcagg ttggtcgcca cctcccaggc
    10741 gtcaaccggc agcgccgcca gggatagagc cgccagcgcg catgacagga gacagcggtc
    10801 ggtgtggatt ctggatttca tctcggtcgt tataaggaga cttttgaact gcgcagtgaa
    10861 aatcgaccgt tctcagaggg gcgattcatg gaaagcattg acattcaaca gtttaatgcg
    10921 cacggggacc atgaaccgat ttgtagccgc cgacgtgagt cggcgctga
//
LOCUS       DAOQRO010000078        10809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5340_length_10809_cov_4.501581, whole genome shotgun sequence.
ACCESSION   DAOQRO010000078 DAOQRO010000000
VERSION     DAOQRO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5340_length_10809_cov_4.501581"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="PKK20_04480"
     CDS             <1..1471
                     /locus_tag="PKK20_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99174.1"
                     /translation="PTSKAPLAAGLRDAYRDAHPDLRTYPPSFAFDDHGITWTPIAAQ
                     EPNDAFDRIDFVYYSDGDGIALLEAFELDDRNSAAPWPSDHRAVLAAFRLTPPEPSPK
                     ATLPWPNDGLTDVACNPTLTWLPGTGALTHDVYFGPTQAIALLTNQTTATFIPGTLEP
                     GATYSWRIDEHTASGIVTGDVWSFTTQPIHYYEWRFIDGNLAPSVGQGVLAYADAATA
                     ALTRFGVADDETVPLMNGQPTRYLYAPAFAGASNGYRVSFEQSGPNGGGIYLNQFTLI
                     WDLLIPSPLGWTPLFNTNPQNANDADFYIDPSGRVGIGALGYSAAGTVRPDTWHRLAF
                     VADFALGTARWYVDGRPVFTGSAALDGRHSLYSNVDPGPDLLLFNEGDTSGVYTHPVC
                     LSAFFMADRPLSAAEVEALGRPSPEGITVRLRPIELAIARDAAHLRLAWIGGGGFYQL
                     QTSPNALPGTWVDMDAPTPDMGVVIEAGATASFYRIAAR"
     gene            1892..2383
                     /locus_tag="PKK20_04485"
     CDS             1892..2383
                     /locus_tag="PKK20_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99175.1"
                     /translation="MRASLTNKLIAGVAATLAVLGVQWFDLTPAARAADRQTGVPAAQ
                     MKDGAGGTAPAATAKTSRYPIRGKLKAVDASGHTFTLAGAEKDRVFKTTAQTEIIRQG
                     KPATLAEAVVGDEVGGLVEKQSNGTVVVLKVRFGPKTEEEQKATTPKRKPRSAAAKTA
                     DVR"
     gene            2566..3648
                     /locus_tag="PKK20_04490"
     CDS             2566..3648
                     /locus_tag="PKK20_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU99176.1"
                     /translation="MGLMDYLKTQLLDIIQWQDDSRDTVSWRFPDEDKEIKRGAQLIV
                     RESQVAQFVYLGQFGDTFGPGKHSLTTDNLPVLSTLKGWKYGFESPFKADVYFVTTRL
                     FTGNKWGTSNPVMMRDADFGIVRVRAFGTYDFRVIEPKLFLKEVAGTDAHFRLDEFSD
                     AMRSRLVSVFSDALASAKVPVLDVATRYSELGEALLPLINPVLAEKYGLEMPSFIVEN
                     VSVPPEVEQAIDKRSSMAAIGNLNDYVKLQMAEGMAKGEGGGGAGSMAAQFAVGMAMA
                     QQMMQQTGGVMAQTTPAIPGAGAPATPAVGAPLPDLLSPADAATLLGVSEADVLTALG
                     EGSLKGKKIGATWRIPRAAVDEFLKS"
     gene            3692..4795
                     /locus_tag="PKK20_04495"
     CDS             3692..4795
                     /locus_tag="PKK20_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNU99177.1"
                     /translation="MVEAEARSKFSCPACGGEAVWNPAKQALICAFCGTASPAKIDVS
                     PSGEEVIRENDLATALRAIPQDRRGWEAPKTSVRCQSCQAISVFDPERVGQRCDFCGS
                     SALVPYQEIKESFRPEGLLPVKVSEAQVRESVRIWYGNRWFAPNRLKSGALTDTVKGL
                     YIPYWTFDAQVHADWTAESGYYYYVAESYRDANGQMKIRQVRKIRWQTSAGALDHFFD
                     DELVGASRGVQADLLRRIEPFPTKELVRYDAGFLSGWVVERYQIDLVAAAQQARERMD
                     TAVRQLCAAQVPGDTHRNLEVRADYSSQTFKHILVPLWLLTYTYGARVFQVMVNGYTG
                     AIAGHFPKSWIKIMLAVLAGLAAMAALFWMTQH"
     gene            4818..5420
                     /locus_tag="PKK20_04500"
     CDS             4818..5420
                     /locus_tag="PKK20_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99178.1"
                     /translation="MEWSIQARSHQCQACGRTFRNKERLHTLLRDERNAYLRVDVCES
                     CWAEHYAEGANHQRGFVSCWKSLYSAPQPTPEPIRRETAETLLRKLLELNAPEHAPAC
                     FILAAMLERKRLLKMKSQTTEQDRRLLIYEHSKTGEVFTISDPNLQLDQLEGVQRGVA
                     RLLEHGLEPPPPTAPEPPRDEPVAPSESPASSPAEPPNHE"
     gene            5413..6129
                     /gene="rnc"
                     /locus_tag="PKK20_04505"
     CDS             5413..6129
                     /gene="rnc"
                     /locus_tag="PKK20_04505"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNU99179.1"
                     /translation="MSDLARLQDEIQYTFRDERLLRTALTHPSVAYEPDQTNDTNQRL
                     EFLGDAVLQLVLSHALYVRHPNAAEGDLTKARARLVNRRSLAEQGRHMELGRYLTLGR
                     GEELHAGRERISTLADAFEALLGALFIDGGYAVAEEFVLRRLAPTLDMLSGPLHADNP
                     KGELQEMLQTGAETPPQYRIEHTSGPDHNMVFECAVYHRGVELGRGIGKSKKEAESQA
                     ASAALRFLGQQPDSAASASD"
     gene            complement(6217..7056)
                     /locus_tag="PKK20_04510"
     CDS             complement(6217..7056)
                     /locus_tag="PKK20_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99180.1"
                     /translation="MIRIALSGVSMAWLLISASAQTLYDGALRSLPVEQDWTFLAFPG
                     GAVQALADDAVCLDTWSSALEQAGYGRTTPIALDRSAGVTVLFAARVDQESHGGPDRA
                     GFSVIALGCDQRGIELGFWTNVVFAQSDLPLFTHAEDALFDTRTAFVDYALTLRRDRY
                     QLRANGKTILEGPARDYTAFSGTIDPYETPDFVFLGDDTTSARAVVWIRRVVLVPAPI
                     LRVSADGTVTWAGVAGVPYTIETSADLAVWTRLASVTSTTGLCTHAVAPGTNARFLRV
                     AGP"
     gene            complement(7167..8444)
                     /locus_tag="PKK20_04515"
     CDS             complement(7167..8444)
                     /locus_tag="PKK20_04515"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNU99181.1"
                     /translation="MTLVLSCLLVLPWLASASLGAADFSSAARAYRRQLGEKVMPYWY
                     DTAVDWRRGGYVLSDDAAKPASPAREKQIVTQARMVWGFAHAHRKGYRSDTRDYLKAA
                     GHGYRFLIERFLDREHGGYYWKTDLDGKPVNDRKYLYGESFVVYALVEYARASGDPGA
                     LRHALDLFRTIQRHCHDATHGGWGEHYERDWRFIARQDDRIEVEVAGLKSANAHLHWM
                     EALAELCEATRDDDVRRALAEALRLNATHFYPPEAGKACFHRNPDWSFVADPRSAGLS
                     YGHNVEFAWLMIRAEEVLERERSWPHFDAILEHALEHGYDHVRGGLYNRGVGDQPAHD
                     TDKVWWSQSEMMAALTDGLRHASNPDYAAALERLVGFLDTHQIRPEDGIWLETVSAEG
                     KPKSTAKAHNWKANYHDVRALVKFIEAFERPAP"
     gene            complement(8537..10558)
                     /locus_tag="PKK20_04520"
     CDS             complement(8537..10558)
                     /locus_tag="PKK20_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNU99182.1"
                     /translation="MREEFEKLVVAGKIGRQHVEMLVTLAASGFCVHRSWGFGQIRQI
                     DTVFGRFNIDFQAKPDHSMDLAFAAESLKAIPPDHILARKATDIAGLRHMAALHHLDL
                     IRLVLQSYGGRATADQIQRVLTPDVITSDWKKWWEAAKREMKKSGHFQIPLKKNEPIV
                     YQVQQVSLKDRLMAEFKSAKGLKARLTVASELLHNLDDLTERETAIADAIAALNVEIQ
                     SHQRTMPALALEAIFMRDDLRAAAGLARGEGELSGQDLWAQIENPGRVLEQIASAKLK
                     RALQSFREAHPALWADHVLGFMNSVGAKLCTECGRLLIEHDRLDALKSALARLISQHT
                     ASSELLLWLAKERSDAFADILGPEVFRAMLSAIERDQFNEKKSNRLRDYILDDQNLLI
                     ELIESADIELIKDLTRTLQLSPSFDDMDKRSLLARIVKHSPVVQSMITGDHSRQDTSL
                     VVSWESLERRKQEYDELVQKKIPANSRDIAIARSYGDLSENHEYKAAKELHRLLMRRK
                     AELESQLVRARGTDFANPRTDVVSIGTRVEVTEAGTGRRETFLILGAWDFDPAKHIIS
                     YLSPIAQSMLNRKVGDEIEFELDGHKSRYRIESLTLPEAPAAKAPGADDAPAAEPGLP
                     APEPAAAPAPGSESAASGREDALAGASADPAGEPADPALAAQSGLNPDR"
     gene            complement(10542..>10809)
                     /locus_tag="PKK20_04525"
     CDS             complement(10542..>10809)
                     /locus_tag="PKK20_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99183.1"
                     /translation="RRFDSGALECADEAVRRVGAGRSAADVAMAVAEPVAVAVDAVGT
                     YGGAGGVLPEDAAGNFSWFLGRHFAIMYIACECKRPMASYERRI"
BASE COUNT         2015 a         3653 c         3292 g         1849 t
ORIGIN      
        1 gcccacctcg aaagcgcccc tggcagccgg ccttcgagat gcctaccgtg acgcgcaccc
       61 ggacctccga acatacccgc cctcattcgc cttcgacgat cacgggatca cctggacgcc
      121 catcgccgcc caggaaccca acgacgcatt cgatcggatc gacttcgtct attactcgga
      181 cggcgacggg atcgcgctgc tcgaggcttt cgaactggac gaccgcaaca gcgccgcgcc
      241 ctggccgtcg gatcatcggg ccgttctcgc cgcattcaga ctgaccccgc ccgagccctc
      301 gccaaaggcc acgctgccgt ggccgaacga tggtctgacc gatgtcgcgt gcaaccccac
      361 cttgacgtgg ctgccgggca ctggagcgct gacccacgat gtctacttcg gcccaacgca
      421 agcgattgcc ctcctcacaa atcagacgac cgccaccttc atcccgggaa cgctcgaacc
      481 gggcgccacc tactcttggc gcatcgatga acacaccgct tcaggaatcg tcacgggcga
      541 tgtgtggtcg ttcaccaccc agccgattca ctattacgag tggcgcttca tcgatggcaa
      601 cctcgccccc tcggtcggcc agggtgtgct tgcgtacgcc gacgctgcca ccgccgccct
      661 gaccaggttc ggcgttgccg atgatgaaac cgtcccgctg atgaacgggc agccgacgag
      721 gtatctctat gctcccgcct ttgccggcgc cagcaacggc tatcgcgttt cgtttgaaca
      781 aagcggcccc aatggcggcg gcatctatct caaccagttc acattgatct gggatctcct
      841 gattccaagc ccgctcggtt ggaccccgct cttcaacacc aacccccaaa acgccaatga
      901 cgccgacttc tacatcgatc cgtccggacg cgtcggcatc ggcgcccttg gatattccgc
      961 agccggtacc gttcgcccgg acacctggca tcgactggcc tttgtggccg acttcgccct
     1021 gggcaccgcg cggtggtatg tcgatggccg accggttttc accgggagcg cggccttgga
     1081 cggccgccat tccctctact cgaacgtcga tcccgggcca gacctcctcc tcttcaacga
     1141 aggcgataca tccggcgtgt acactcatcc ggtctgtttg agcgcgttct tcatggccga
     1201 tcgcccgctc agcgccgccg aagttgaagc ccttggcaga ccttcgcccg agggaatcac
     1261 agtcagactg cgtccgatcg aattggcaat tgctcgggac gcggcacact tgcgcctggc
     1321 gtggatcggc ggcgggggat tctaccagct ccagaccagc cctaacgcgc tgccgggaac
     1381 ctgggtcgac atggatgctc ccacccctga catgggtgtc gtcatcgaag ccggcgcgac
     1441 cgcttcgttc tatcggattg ccgcgcgcta gcccggaaac ctgatgccgg gacgagaatc
     1501 cggatttccg ggctgtggca agaccgcgcc ccaacgcaga gaggcacgcg gcggggtcaa
     1561 gcacacaaac catggacggt catcactcgt gcgtagatga ccttgccccc tccggtaaaa
     1621 cgttgccggc gtgtcgagcg acaaaggtgg cggcgagggt ggcgacagag aaaaggaacg
     1681 gggggccgct tgcatttctc caaaggacga ttatcttctg cggcaagttg gaggagggat
     1741 ggttcaccca aatggcagga cgaattgcgt ttacgatctc gagcgcccat gtcgcgcctc
     1801 ggaatccaag ccccggtgaa ccgatcgcgt cgttccccgg cctggcttgt ccggccgcca
     1861 atccgtcgtc caacccaaca aggaggaaac catgcgtgca tcactcacga acaaactgat
     1921 cgccggcgtc gcggccacac tcgcggtgct gggcgtccag tggttcgatc tcaccccggc
     1981 ggctcgggct gccgaccgcc aaaccggggt tcccgcagcc caaatgaagg acggcgccgg
     2041 cggcacggcc cccgctgcga ccgccaagac gagtcggtac ccgatccgcg gcaaactcaa
     2101 ggccgtcgac gcctctggcc acacgttcac gctggccggc gccgagaagg atcgggtctt
     2161 caagaccacg gcgcagaccg agatcatacg gcagggcaag ccggcaacgc tggccgaggc
     2221 ggtcgtcggc gacgaggtcg gcggccttgt cgagaagcag agcaacggca ccgtcgtcgt
     2281 gctgaaggtg cgcttcgggc cgaagacgga ggaagagcag aaggccacaa ccccgaaacg
     2341 caagccgcgt tcggccgcgg cgaagacagc cgacgtgcgc tgagttgacg gcacgtcatc
     2401 gggcgggcgg gccgcttatg gcctgcccgc ctctctcccg cgcagcccct tgaccatcat
     2461 gcacggaccc ttacacaaac ctgaggatat tttacttctc atgccgcgcg ttccggctat
     2521 gaatttggcg aatcggttta tggcggacga tcgaaaggat tcacaatggg actcatggat
     2581 tatctgaaga cgcaactgct tgacatcatc cagtggcagg atgattcgcg cgacacggtt
     2641 tcctggcggt tcccggacga ggacaaggaa atcaagcgcg gggcgcaact catcgtgcgg
     2701 gagtcccagg tggctcagtt tgtctatctg ggccagttcg gagatacatt cgggccgggg
     2761 aaacactccc tcaccacgga caacctcccc gttctatcga cgctcaaggg ctggaaatac
     2821 ggcttcgagt ctccattcaa ggccgacgtc tacttcgtca cgacccgcct cttcaccggc
     2881 aacaagtggg gcacatccaa tccggtcatg atgcgtgacg ccgatttcgg catcgtgcgc
     2941 gtgcgcgcgt tcggcaccta cgatttccgg gtgatcgagc ccaagttgtt cctcaaggag
     3001 gtagccggca ccgacgcgca cttccggttg gatgaattct ccgacgccat gcgttcgcgg
     3061 ctggtgagcg tgttctccga cgccctggcg agcgccaagg tgcccgtgct cgatgtggcc
     3121 acgcgctact ccgagctggg cgaggccctc ctgcccctca tcaaccccgt tctcgccgag
     3181 aagtacggac tcgagatgcc cagtttcatc gtcgagaacg tctcggtccc gcccgaggtg
     3241 gaacaggcca tcgacaaacg gtcgagcatg gccgccatcg gcaacctcaa cgactacgtc
     3301 aagctccaga tggccgaagg catggccaaa ggcgaaggcg gcggtggcgc cggcagcatg
     3361 gccgcacaat tcgcggtcgg aatggccatg gcccagcaga tgatgcagca aaccggtggt
     3421 gtcatggccc aaaccacgcc cgccatcccc ggagccggag cccctgcgac gcctgcggtc
     3481 ggcgcgcccc tgcccgatct gctcagcccg gccgacgccg ccacgttgct cggggtcagc
     3541 gaggccgacg tcctcaccgc gctgggcgag gggtctctga agggcaagaa gatcggcgcc
     3601 acctggcgca tcccccgtgc tgccgtcgac gagttcctga agtcttagga aggcgcgcct
     3661 cagaccactc cacgcttggg ggattcgcac catggttgag gcagaggccc gatcgaagtt
     3721 ctcctgtccg gcctgcgggg gagaggccgt atggaatccg gccaagcagg ccttgatctg
     3781 cgcattttgc ggcacggcct cgccggcaaa aatcgacgta tcgccctccg gcgaggaggt
     3841 gattcgggaa aacgaccttg caaccgccct gcgggcgatt ccgcaggatc gccgcgggtg
     3901 ggaggcgccc aagaccagcg ttcgctgtca gagttgccag gccatctcgg tgttcgatcc
     3961 cgagcgtgtc ggccagcgct gcgacttctg cggatcttcg gccctggtcc cctaccagga
     4021 gatcaaggag tcattccggc ccgaggggct cctgcccgtg aaggtcagcg aagcccaggt
     4081 gcgcgagagc gtccggatat ggtacgggaa ccggtggttc gcccccaaca ggctcaagtc
     4141 cggcgccctc accgacacgg tcaagggact ctacatccca tattggacct tcgacgccca
     4201 ggtgcacgcg gactggacgg cggaatccgg ttattactac tacgtggccg aaagctatcg
     4261 cgatgcgaac ggccagatga agatacggca agtccgcaag atccggtggc agaccagcgc
     4321 gggggccctc gaccacttct tcgacgacga gcttgtcggg gcctcgcgcg gcgtccaggc
     4381 cgacctgctc cgccggatcg agccctttcc cacgaaagaa ctggtccgtt atgacgccgg
     4441 gttcctctcg ggctgggtgg tcgagcgcta ccagatcgac ctcgtcgccg ccgcccaaca
     4501 ggcccgcgaa cgcatggata cggctgtccg gcagctctgt gccgcccagg tccccggcga
     4561 tacgcatcgc aacctcgagg tccgcgccga ctactcgagc cagaccttca aacacatcct
     4621 ggttccactc tggctcctga cctacaccta cggagccagg gtgtttcagg tcatggtcaa
     4681 cggctatacc ggcgccatcg ccggacactt cccgaagagc tggatcaaaa tcatgctcgc
     4741 cgtcctcgcc gggctcgccg cgatggctgc cctgttctgg atgacgcagc attaacgccg
     4801 cgtcccgcca cactctcatg gaatggagca tccaggcccg atcgcatcag tgccaagcgt
     4861 gtggccgcac attccgcaat aaggaaaggc tgcacaccct cttgcgggac gaacgcaacg
     4921 cctacctccg cgtcgacgtt tgtgagtcgt gctgggccga gcactacgcc gaaggcgcca
     4981 accatcagcg cggctttgtc tcgtgctgga aaagcctgta ctcggccccg caaccgacgc
     5041 ccgagcccat ccgccgggaa accgctgaaa ccctcctgcg caagctcctc gaactcaatg
     5101 cgcccgagca cgccccggcc tgcttcatcc tggctgccat gctcgaacgc aaacgcctcc
     5161 tcaagatgaa gagccagacg accgaacagg accgtcgcct tctcatctac gaacattcaa
     5221 agacaggcga ggtgttcacc atctccgacc ccaacctcca gctcgaccaa ttggagggcg
     5281 tccagcgcgg cgtcgcccgc ctgctcgagc acggactgga accaccgccg cccaccgcgc
     5341 ccgagccgcc ccgggacgag cccgttgccc caagcgaaag cccggcctcg agcccggccg
     5401 agcctcccaa ccatgagtga tctggcccga ttgcaggacg agattcaata cacgtttcgg
     5461 gatgaacggt tgctgcgcac tgccctcacg catccgtccg tcgcctacga accggatcaa
     5521 accaacgaca ccaaccaacg cttagaattc ctcggcgatg ccgtgcttca actggttctc
     5581 agccacgccc tctatgtccg gcacccgaat gccgccgaag gcgacctgac caaagcccgc
     5641 gcccggctgg tcaaccgtcg ttctctggcg gagcagggcc gacacatgga gctgggccga
     5701 tacctgacac tgggacgcgg cgaggaactg catgcggggc gcgagcggat ctcgaccttg
     5761 gcggacgcct tcgaagccct gctgggcgcg ctcttcatcg atggaggcta cgccgtcgcg
     5821 gaggagttcg tcctgcgacg cctcgcaccc acgctcgaca tgctctccgg tccgctgcac
     5881 gccgacaacc caaagggcga acttcaggaa atgctccaga ctggcgccga gacaccgccg
     5941 caataccgca tcgaacacac ctcgggcccg gaccacaaca tggtgttcga gtgcgcggtc
     6001 tatcaccggg gcgtggaatt gggtcgcggc atcggcaaaa gcaagaagga agccgagagc
     6061 caggccgcat ccgccgccct tcggttcctg ggccagcagc cggacagcgc ggcgtccgcc
     6121 tcggattgag agcggatcgc ctcaacgttc agtccgctcc gtacgttcca tcgcccggtc
     6181 cgcctgaacc cattgacacg atccattcgc cagcgctcat ggccccgcca cacgcaagaa
     6241 acgcgcgtta gtgcctgggg ccacggcgtg cgtgcaaagg cctgtggttg aggtgactga
     6301 cgccaatcga gtccagactg ccagatcggc gctggtctcg atggtgtagg gaacgccggc
     6361 tacgcccgcc caggtcaccg tgccgtcggc ggaaacgcgc aggatcggcg ccggcacgag
     6421 aaccacacgg cgaatccaga cgaccgctcg cgccgacgtg gtgtcatcgc caagaaagac
     6481 gaagtcgggc gtttcgtacg ggtcgatggt gccggagaag gcggtgtaat cgcgcgccgg
     6541 gccttcgaga atcgttttgc cgttggcccg gagttggtag cgatcgcgcc ggagggtcag
     6601 cgcgtagtcg acaaaggccg ttcgggtgtc gaacagagca tcttccgcgt gggtgaagag
     6661 cggcagatcg gactgggcaa agaccacgtt ggtccagaaa cccaactcga tcccgcgctg
     6721 gtcgcacccc agcgcgatca ccgagaatcc ggcgcggtcg ggaccaccgt gcgattcctg
     6781 atcgacccgc gcggcaaaca gcaccgtcac acccgcgctc cggtccagcg cgatgggtgt
     6841 cgtgcgcccg tacccggcct gctcgagcgc gctgctccag gtgtcaagac agaccgcgtc
     6901 atcggccagc gcctggaccg ctccgcctgg aaaagcgagg aacgtccagt cttgttcgac
     6961 aggcagggag cgcagcgcgc cgtcgtagag ggtctgcgct gacgccgaga tcaagagcca
     7021 ggccatcgag accccgctca gagcgatcct gatcatacgc cttgtgtccc tgcagggagg
     7081 cggcgcgcgc aacatccgcc gtcgcagccc acgacatcga gcccgattct tcgaaacgcg
     7141 acgctcatcg ccaactcatc gtcgagtcac ggcgcaggcc gttcaaacgc ctcgatgaac
     7201 ttcacgaggg cgcggacatc gtggtagttc gccttccaat tgtgggcctt ggccgtgctc
     7261 ttgggcttgc cctcggcgct cacggtttcg agccaaatcc cgtcttcggg ccgaatctga
     7321 tgggtgtcga gaaaccccac cagccgttcc agagcagccg cgtagtccgg attggacgca
     7381 tgccgcaagc cgtcggtcag ggccgccatc atctccgact ggctccacca caccttgtcg
     7441 gtgtcgtggg cgggttggtc gccgacgccg cggttgtaga gcccgccgcg gacgtgatcg
     7501 tagccgtgtt cgagagcgtg ctcgaggatg gcgtcaaaat gcggccacga gcgctcgcgc
     7561 tcgagcactt cctcggcgcg aatcatcagc cacgcaaact cgacgttgtg gccgtacgaa
     7621 agacctgcgc tccggggatc cgccacgaac gaccagtccg gattccgatg aaaacaggcc
     7681 tttcccgcct cgggagggta gaagtgcgtc gcgttgagcc gcaacgcctc ggcaagagcc
     7741 cgccgcacat cgtcgtcgcg cgtcgcctcg caaagctcgg ccagcgcttc catccagtgc
     7801 agatgcgcat tggcgctctt gagcccggcg acctcgacct cgatgcgatc gtcctgtcgg
     7861 gcaatgaagc gccagtcccg ctcgtaatgc tcgccccagc cgccgtgcgt tgcatcgtgg
     7921 caatgccgct ggatggtccg aaacagatcc agggcgtgcc gtagcgcgcc gggatcgccg
     7981 cttgcgcggg catattcaac cagggcgtag accacaaacg actcgccgta caggtacttc
     8041 ctgtcattga ccggcttccc gtccagatcg gtcttccaat agtagccgcc atgctcacga
     8101 tcgagaaaac gctcgatcaa gaaacggtaa ccgtgcccgg ccgccttcag ataatcccgc
     8161 gtgtcgctcc gatagccctt tcgatgggcg tgggcgaatc cccaaaccat gcgggcctgg
     8221 gtcacgatct gcttctcccg cgcaggcgac gccggcttgg ccgcatcatc cgacaacaca
     8281 tagccgccgc gccgccaatc caccgccgtg tcataccagt agggcatcac cttctcgccc
     8341 aactgccgcc tgtaagcgcg ggccgcggac gagaaatccg ccgcacccag agacgcggag
     8401 gcaagccagg gcaggaccag caggcaggac aagacaagcg tcataagtga aagacgtgag
     8461 gagcgggagc gcctgtcagg acttgatcgc cacatgcgca gggcccagcc ggggtatttc
     8521 ataaggctgt gcggagttat cgatccgggt tgaggcctga ctgagccgcc aaagccgggt
     8581 ccgccggctc gccggcgggg tcggcggaag ccccggccag ggcatcctca cggccagagg
     8641 ccgcgctttc ggagccgggg gcgggggcgg ccgcgggttc gggcgcgggc aggccgggct
     8701 ccgcggcggg cgcgtcgtcg gcgcctggag ctttcgcggc gggcgcctcg gggagggtca
     8761 gcgactcgat gcggtagcgg ctcttgtgcc cgtcgagttc gaactcgatc tcgtcgccga
     8821 ctttccgatt cagcatcgat tgggcgatcg gactcaggta cgaaatgatg tgcttggcgg
     8881 gatcgaaatc ccacgccccc agaatgagaa aagtctcccg gcgtccggtg ccggcctcgg
     8941 tgacctcgac gcgggttccg atgctgacga cgtcggtgcg cgggttggcg aagtcggtgc
     9001 cgcgggcccg gacaagctgg ctttcgagtt cggccttgcg gcgcatgagg aggcggtgga
     9061 gttccttggc ggccttgtac tcgtgatttt cgctgaggtc gccgtagctt cgggcgatgg
     9121 cgatgtcgcg cgaattggcg gggattttct tctggaccaa ctcgtcgtac tcctgcttgc
     9181 gccgttccag gctctcccac gaaaccacca gcgaggtgtc ctgcctgctg tggtcgccgg
     9241 tgatcatcga ctggacaacc ggcgaatgct tgacaatgcg cgccagcaac gaccgtttgt
     9301 ccatgtcgtc gaagctgggc gaaagctgca gcgttcgggt gagatccttg atgagctcga
     9361 tgtcggcgct ctcgatcaac tcgatcagca gattctgatc atccaggatg tagtcgcgca
     9421 accggttgga tttcttttcg ttgaactggt cgcgctcgat ggcgctgagc atggcgcgga
     9481 acacctccgg acccagaatg tccgcaaagg cgtcggaccg ttctttcgca agccagagaa
     9541 ggagttcgct gcttgcggta tgctggctga tgaggcgggc cagggcgctc ttcagcgcgt
     9601 ccaggcgatc gtgctcgatc aacaatcggc cgcattcggt gcagagcttc gccccaacgg
     9661 agttcatgaa acccaacaca tggtccgccc acagggcggg gtgagcctcg cgaaaggact
     9721 gcagcgcacg cttgagcttg gccgacgcaa tctgctcgag cacgcgcccg ggattctcga
     9781 tctgggccca caggtcctgg ccgctcaact cgccctcacc gcgcgccagg ccggcagccg
     9841 cacgcaggtc atcgcgcatg aaaatcgcct cgagcgcaag ggccggcatg gtccgctgat
     9901 gggactggat ttcaacgttc agcgcggcaa tggcgtccgc gatggccgtc tcgcgttccg
     9961 tcagatcgtc caggttgtgc aggagctccg aagccacggt caggcgggcc ttcaatccct
    10021 tggcagactt gaattcagcc atcaaccggt ccttcagcga aacctgctgg acctgataca
    10081 cgatcggctc gttcttcttg agcgggatct gaaaatggcc gctcttcttc atttcgcgct
    10141 tcgcagcctc ccaccacttc ttccagtccg acgtgatcac atccggcgtc agcacccgct
    10201 ggatctgatc cgccgtcgcc cgtcccccat aactctgcaa aacgagccgg atcaggtcca
    10261 ggtgatgcag cgcagccatg tggcgcagtc cggcaatgtc cgtcgccttc cgggcaagga
    10321 tgtggtccgg aggaatggcc ttgagcgact cggcggcaaa cgccagatcc atggaatggt
    10381 cgggtttggc ttggaaatcg atgttgaacc ggccaaacac cgtgtcgatt tgcctgatct
    10441 ggccgaaccc ccaactgcga tgcacgcaga aaccagaggc ggcgagcgtc accagcatct
    10501 ccacatgctg gcgtccaatc ttgcctgcca ccacgagctt ttcaaattct tctctcatat
    10561 gaggccatcg gtcgcttaca ctcgcaagcg atgtacatta ttgcgaagtg acggcccaaa
    10621 aaccaagaga aattgcctgc cgcatcctct ggcagcacgc caccggcccc gccgtacgtc
    10681 cccaccgcgt ccaccgccac cgcaaccggc tccgccaccg ccatcgcgac gtcggcggcc
    10741 gagcggccgg cgcccacccg ccgcaccgcc tcgtcggcgc actcgagcgc acccgagtcg
    10801 aagcgacgc
//
LOCUS       DAOQRO010000079        10784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5359_length_10784_cov_4.285674, whole genome shotgun sequence.
ACCESSION   DAOQRO010000079 DAOQRO010000000
VERSION     DAOQRO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_10784_cov_4.285674"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKK20_04530"
     CDS             <1..1059
                     /locus_tag="PKK20_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNU99184.1"
                     /translation="TDKSVWFQGRAGWMFATLFNTIETRAEWLAASRSCLDFSRRHCF
                     APDGKMYFTVTREGRPLRMRRYVFSESFAAIGCAAFAKAANDARAADEAVKAFAAYLH
                     HSFTPGIMPPKSEPTRPAKAIGPLMIAIGAAQELRANLGDVEISGRTCTEWIDSSIAE
                     IERDFLKPEHQALLEVVAPDGALLDHYDGRMLNPGHALECAWFVLHEGALRQDRRLVQ
                     LGATIVDWMWARGWDPEHGGLLYFTDLRRLPVQEYWHDMKFWWPHAEAIIATLLAWEL
                     TREPRFAQRHRLVHDWSFARFPDLEYGEWYGYLHRDGSVSSRAKGTLWKGPFHLPRML
                     WYCSRLLEQSGTAATQCS"
     gene            1062..3425
                     /locus_tag="PKK20_04535"
     CDS             1062..3425
                     /locus_tag="PKK20_04535"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HNU99185.1"
                     /translation="MIVRSILTCFLSAVRRGVAPWLGATVIAATASANVVQLENQSLV
                     LGVDSHNGRILELADRASGQDFANAAHPGDLWELEFDGAPPRTVAPADARVFRIERLR
                     GSDRGLRLDWSGFGLPDAPALRIEVIVRLDRRRALSRWQLAVDAPGERTIQRIRFPRV
                     HRLPRLERECLAVPFWAGLLARNPRHIFAGGAPGGVRREYDYPGHCSMQCLAFYSESG
                     PGLYFACDDTAGYRKAFAAFSTGAEGPVLRPNPGVDESVAPEAGEAPDLNLEIVHLPD
                     REEAVGRRYTLPYSAIIGAFQGKWFDAAEIYRAWATNQTWARESRWKRKAVPHWIADT
                     GLWIWNRGRSPGVIEPAVAFQREIGLPVSVFWHWWHGCAYDTGFPEYLPPREGNASFM
                     AALDRAHASDVRALVYMNQRLWGMTTESWTNENAATYAVKAADGRVRPEVYNVFTKLP
                     CATMCLGTEFWRSKYAGLAIEAFNRLGVDGIYMDQACSSLVCLDSQHGHPPGGGTYWM
                     NGFKALESDIRQRCPTRGGVALAGEGCAENWLPHLDLMLALDVSRERYAAPDGWEPIP
                     FFHAVYHGYGVFYGNYSSLTMPPYDDLWPAEFAPQKPLELLDRRFSRQFYLEQARSFI
                     WGQQPTLANFQPSQFRERPDEIAYVMRLARLRKLALDYLQDGAMLPPPNVETPHQEIP
                     ISRLSIYAGQREALKEFQMTVPLVLASAWRAVNGRMAVAVASIADQPLTPTIRLDAAR
                     CGLSRRARVEPIGEPPPIACKTGFDGENLELRPALAPLEARVWTLDP"
     gene            3582..4988
                     /locus_tag="PKK20_04540"
     CDS             3582..4988
                     /locus_tag="PKK20_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99186.1"
                     /translation="MHNHITPTHTGHPSHHASAPSQAACSRREFLAQSGKAVAVSALA
                     GVSLPAVHAAEDNTIRLALIGCGGRGSGAAVDAFESKQGPVKLVAMADFFENRLSNSY
                     QNLHSKYADRMDVPPDRRFVGFDGWRKAIDALRPGTGDIAMLCGYAGFRPGQLEYAVE
                     RGVNVFMEKSFAADPPGVRRVIRAGEAAAAKNLKIAAGLQCRHSKNRQELIRRIREGE
                     LGQIQLIRAYRMQPCGWLGPRPETEKDLHWQVRNFVHFLWVSGGLFAEMNIHQIDEIC
                     WLKGEWPVTAHGVGGRAANSTDCGQNLDSLAIEWTFADGTKATDVVRWLPKCHEEFAT
                     YLHGTRCAAQFSGNIHEGTVHTYRDQRCANDNIAWRAPKEDVSPWQAEWDDLLESIRL
                     DRPHNEAVRAARSNLADMMGRAAVHMGRVVTWDEAMNSSFQFCPNIDQLTTDSPPPIV
                     ADAQGRYPVPVPGAWTEL"
     gene            5057..6241
                     /locus_tag="PKK20_04545"
     CDS             5057..6241
                     /locus_tag="PKK20_04545"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNU99187.1"
                     /translation="MRQAKTPSFQLGSLMAGCTSLALAWMAGTPGALAKSTDDLVYVG
                     TYTGSKSKGIYAFRFNAKDGRAETPFLAAETAHPTFLALAPNKRFLYAVGELGTFQGK
                     KAGSVSAFSIDRATGRLDLLNQVSSGGPGPCHLVLDATGKWVLVANYGGGSVALLPIQ
                     GNGALGEAVSFHQHQGSSLNPQRQEGPHAHGVTLDAANRVAIVPDLGLDRLMIYRLDA
                     VNGRLTPNDPPHAAVKPGSGPRHLAFAPDYKHAYGINELSSTITVYAYDSAGGSLAEV
                     QTLSTLPDGFDGDNSTAEIEVHPTGKFVYGSNRGHDSIAVFARDLQRGTLERVANVPT
                     QGKAPRNFAIDPTGAWLWAANQGSDNLVLFRIDPERGLLQPAGVTVDIGSPVCVQYVP
                     LD"
     gene            6424..10131
                     /gene="mfd"
                     /locus_tag="PKK20_04550"
     CDS             6424..10131
                     /gene="mfd"
                     /locus_tag="PKK20_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNU99188.1"
                     /translation="MVVTTDLKAQESLHQDIQTWLTADAPTDPIRPGPSCRALFYPAW
                     ESLPHQGQLPHADIISERLETLVALAEHRERSNRPRKRDRNSESESARSLDPGSASSL
                     TSGGPEHPPAPVDQIPPAPLVVTSVGALLQRTFPSDWLRARHRVLRRGDSLAPLDLIE
                     WLEDQAYEPEAQVTQKGELALRGGIVDVFPLTSPWPVRLEFFGDTLESLRLFDPQTQI
                     SREEIEQITLAPAGEIGLLRQLLDASAHVASARDANADALPAGPTAPAQPQRVSAGSD
                     HPAAPFPVSEARLASFLDHLGPDALVVFCDPIALDEQAERYAEQIPPGDPFHVNWHQV
                     QADIEARALTRIALFETEFGQGAAPDDRLPGWTGPALDPTTRDPNQANMTFTSLDVYR
                     PLGDRQPDPEVAEAQRRQFFTQLHRWLRQGFAVEVFCNNTGEQQRLSELCRESGLAEL
                     ETRLGTLSRGFLCDAARVVVVTDAEIFGRYKVQRPRRLKSPHAVAMRSAFDIDFTDLA
                     EGDYVVHLQYGIGRYLGLQRLPSGRGRKGATTNLEEPGDGSGGEECLVIEYAPGGPKE
                     DAPKLYVPVTESHLVSKYVGAGRARPPLNTLSGSHWLKAKAQANAAVRDFAAELLRLQ
                     AARSVQSGFAFPPDAPWQLEFESAFVYEETPDQLRAIEATKQDLERPRPMDRLICGDV
                     GFGKTEVAIRAAFKVVLAGRQVAVLVPTTVLAHQHYNTFRERMAGYPIRIELLSRFRT
                     RRQQNRIVRDLAVGGVDIVIGTHRLVQPDVVFKDLGLVVIDEEQRFGVAHKERLKRLR
                     QMVDVLTLSATPIPRTLYLALMGARDMSTIETPPQDRLPVETVIAPYDERLIRDAIRR
                     ELNRGGQVFYLHNRILDIEAVAKRLGQLVPQARLAVGHGQMHADALEEVMTQFVNGEI
                     DVLLSTTIIESGIDIPNANTIIIDRADRFGLGDLYQLRGRVGRYKHQAYAYLLIPRHA
                     GLLADARKRISAVRQFSSPGSGFKIAMRDLEIRGAGNLLGPEQSGHITAVGFELYCRL
                     LKHSIAALNGEPLKPLRTVSVRFDFLDLSPVGHEESDRAAPVSPATHEAPQPDAPPPA
                     RMDAKAKTTPPAPPRAARGSGSASACLPLRYVPEPHHRIEMYRKLAQVTDKAGLDAVR
                     SELRDRFGPPPPAVELLVQVSALKILAADRGLDAMETRDDRLMLTRNGQFIQVGGRFP
                     RLTKKTAAARLNEIRKILLALEPCPLGRVSP"
     gene            complement(10418..>10784)
                     /locus_tag="PKK20_04555"
     CDS             complement(10418..>10784)
                     /locus_tag="PKK20_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99189.1"
                     /translation="LEERWFPFMVFLVGLCVYVGLPALVCALAFRGGLVLRALGVAIV
                     RHDGAPASRLRVFWRGLMAWSPVLLAPLLFGMLRGPLGPFGAAVLSSLLAIGLTALSL
                     AHPQRNLQDRFAGTWLVPR"
BASE COUNT         1974 a         3832 c         3157 g         1821 t
ORIGIN      
        1 accgacaagt cggtctggtt ccagggacgc gcaggctgga tgttcgcgac cctgttcaac
       61 accatcgaga cacgcgccga gtggctcgcg gcgtcacgat cgtgcctcga cttcagccgc
      121 cgacactgct tcgccccgga cgggaagatg tacttcaccg tcacccgtga aggccgcccg
      181 ctgcgcatgc gccgctacgt gttcagcgag agcttcgccg ccatcggctg cgccgccttc
      241 gcgaaagcgg cgaacgacgc ccgcgcggcg gacgaggccg tcaaagcgtt tgcggcctac
      301 ctgcaccaca gctttacccc cggcatcatg ccgccgaagt ccgagccaac ccgcccggca
      361 aaggcgatcg gcccgcttat gatcgccatc ggggcagccc aggaactgcg ggcgaacctg
      421 ggcgatgtcg aaatctccgg acgcacctgc accgagtgga tcgactcgag catcgctgaa
      481 atcgagcgcg attttcttaa gcccgagcac caggcgttgc tcgaagtcgt cgccccggac
      541 ggcgcactcc tcgaccacta cgacgggcgc atgctcaacc ccggccacgc cctcgaatgc
      601 gcctggttcg tcctccacga aggcgccctc cgccaggacc gcaggctcgt ccaactcggc
      661 gcaaccatcg tcgactggat gtgggcacgt ggctgggacc ccgaacatgg cgggttgctc
      721 tacttcaccg acctgcgccg cctgcccgtg caggagtatt ggcacgacat gaaattctgg
      781 tggccccacg ccgaggccat catcgccacc ctcctggctt gggaactcac ccgcgagccg
      841 cgcttcgccc agcgccatcg cctggtccac gactggagct tcgcccggtt ccctgacctc
      901 gagtacggcg aatggtacgg ctacctccac cgggatggct cggtttcctc acgcgccaaa
      961 ggcacacttt ggaaaggccc cttccacctt cctcgaatgc tctggtattg cagccgcctc
     1021 ttggagcagt cgggcacagc cgcaacccaa tgttcgtgaa catgattgtt cgctcgattc
     1081 tgacctgttt cctctccgcg gtcaggcgcg gagtcgcgcc ttggctcggc gccacggtga
     1141 tcgccgccac cgcgtcggca aacgtcgtcc aactcgaaaa ccaatcgctc gtcctgggcg
     1201 tcgactccca caatggccgc atcctcgagt tggccgatcg cgcctcaggc caggacttcg
     1261 caaatgccgc ccatccagga gacctctggg aactcgaatt cgacggcgcg ccgccgcgaa
     1321 ccgttgctcc cgccgacgcc cgcgtctttc gcatcgagag actgcgcggc tcggacaggg
     1381 gcttgcgcct cgactggagc ggttttggcc ttcccgatgc gcccgcgctt cgcatcgagg
     1441 tgatcgtccg cctcgatcga cggcgcgccc taagccgctg gcaactggct gtcgatgccc
     1501 ccggcgaacg aacgatccaa cgcatccgct tccctcgtgt gcatcgcctg ccccggctcg
     1561 aacgcgaatg cctggccgtg cccttctggg ccggcctgct cgcccgcaat ccccgccaca
     1621 tcttcgccgg cggcgccccg ggcggcgtgc gacgcgaata tgactaccct ggccactgct
     1681 cgatgcaatg cctggccttt tactcggaat cgggaccggg actctacttc gcctgtgacg
     1741 ataccgccgg ttatcgcaaa gccttcgcgg ccttcagcac aggggcggaa ggcccagtct
     1801 tgcgaccgaa cccgggagtc gacgagagtg ttgcgcctga ggcgggcgag gcgcccgatc
     1861 tcaacctcga gatcgtgcat cttccggaca gggaggaagc ggttggacgg cgctacaccc
     1921 tgccatactc cgctatcatc ggcgcattcc agggaaagtg gtttgacgcc gccgaaatct
     1981 atcgcgcctg ggcaaccaac cagacctggg ctcgcgagag ccgatggaag cgcaaggccg
     2041 tcccgcactg gattgccgac accggcctct ggatctggaa tcgcggtcgt tcgcccgggg
     2101 tcatcgagcc cgcagtggcc tttcagcgtg aaatcggcct cccagtcagc gtcttctggc
     2161 actggtggca cggttgcgcc tacgacacag gtttccccga gtacctgccg ccccgcgaag
     2221 gaaatgcgtc attcatggcg gccctggacc gggcgcacgc cagcgacgtg cgtgcgttgg
     2281 tctacatgaa ccagaggctc tggggcatga ccacggaaag ctggaccaac gaaaacgccg
     2341 caacctatgc cgtcaaggcc gccgacggcc gggtgcgacc cgaggtttac aatgtcttca
     2401 ccaagttgcc ctgtgccacg atgtgccttg gcaccgaatt ctggcgctcg aagtacgccg
     2461 gattggcgat tgaagccttc aaccgcctcg gcgtggacgg catctacatg gaccaggcgt
     2521 gcagcagcct cgtctgcttg gattctcagc atggacatcc ccccggcggc ggcacctact
     2581 ggatgaacgg cttcaaggcc ctcgaaagcg atatccgcca gagatgcccg acacgcggtg
     2641 gcgtggcgtt ggccggcgaa ggctgcgcgg aaaactggct gccacacctc gatctcatgc
     2701 tcgctctgga cgtgagccgc gagcgatacg ccgcgccgga cggctgggag ccaatcccat
     2761 tcttccacgc cgtgtaccat ggatacggcg tcttctacgg caactactcg tcactcacga
     2821 tgccgcctta tgacgacctt tggcccgccg agttcgcgcc ccaaaaaccg ctcgaactcc
     2881 tcgatcgccg cttctcgcgc cagttctacc tcgaacaggc ccgctcgttc atctggggac
     2941 agcaaccaac cctcgccaac ttccagccct cccagttccg cgaacgccca gacgaaatcg
     3001 cctatgtcat gcgccttgcg cgcctgcgca aactcgccct cgactacctc caggacggcg
     3061 ccatgcttcc ccctcccaac gtcgagacgc cacaccagga aatcccgatc tcgcgcctct
     3121 cgatctacgc cggtcaacgg gaggccctaa aggagttcca gatgacagtg cccctggtgc
     3181 tcgcgtcggc ttggcgcgcc gtgaacggca ggatggcagt tgccgttgcg agcatcgccg
     3241 atcagccgct gacccccaca atccgtctcg atgcagccag atgcggtctg tccaggcgcg
     3301 cccgcgttga accgatcggt gaaccccctc ctattgcgtg caagaccgga ttcgacggag
     3361 agaacctcga acttcgccct gccctggctc cgctcgaagc gcgagtgtgg acgctcgatc
     3421 cataacgagg cgcgcctcag accagattca gtcctcctct ccatatctcc aacctccaca
     3481 acagcaaccc ttcaggaatt taaatgaaat caccgtggcg gcgcagccca gccctacgaa
     3541 tccgtctcga caacgcggct gcatgaggcg acaacatctc catgcacaat cacattacgc
     3601 caactcacac cggacaccca tctcaccacg catccgctcc cagccaggcg gcctgctcgc
     3661 gtcgcgagtt tctcgcccaa agcggcaagg ccgtcgccgt ctccgccctg gcgggcgtct
     3721 ccctccccgc agtccatgcc gccgaagaca acaccatccg cctggcgttg atcggatgcg
     3781 gcggacgcgg cagcggcgcg gccgtggacg ccttcgagtc gaagcagggg ccggtcaaac
     3841 ttgtggcgat ggccgacttt ttcgagaacc gcctgtccaa ttcctatcag aacctccact
     3901 cgaagtacgc cgaccgcatg gatgtgcccc ccgatcgccg ctttgtgggc ttcgacggct
     3961 ggcgcaaggc catcgatgcg ctgcgtccgg ggacgggtga catcgccatg ctgtgcgggt
     4021 atgcgggatt tcgcccgggc cagttggagt acgctgtcga acgcggtgtg aacgtgttca
     4081 tggagaaatc gtttgccgcc gatcctccgg gcgtgcgccg cgtcatccgg gccggcgaag
     4141 ccgccgctgc gaagaatctc aagatcgccg ccggactcca gtgccgccac tcgaagaacc
     4201 gtcaggaact gatccgccgc atccgcgagg gcgaactcgg gcaaatccaa ctcatccgcg
     4261 cgtaccggat gcagccgtgc ggctggctgg ggccgcgccc ggaaaccgag aaagacttgc
     4321 actggcaggt tcggaatttc gtccactttc tctgggtctc tggcggtctc tttgccgaga
     4381 tgaacatcca tcagatcgac gaaatctgct ggctcaaggg cgaatggccc gtgaccgccc
     4441 acggcgtcgg aggtcgggcg gccaacagca ccgattgcgg ccagaacctc gactcgctgg
     4501 cgatcgagtg gacgtttgcc gacggcacaa aggcgaccga tgtggtgcgc tggctgccca
     4561 aatgccacga ggaattcgcc acctatctgc acggcacacg ctgcgccgcc cagttctccg
     4621 gcaacatcca cgaaggcacc gtccacacct atcgcgacca gcgctgcgcc aacgacaaca
     4681 tcgcctggcg ggcaccaaag gaggacgtct cgccctggca ggcggagtgg gatgacctgc
     4741 tcgagtccat ccgcctcgac cgcccacaca acgaagccgt ccgcgccgca cggtctaatc
     4801 tcgccgacat gatggggcga gcggcggtcc acatgggccg cgtggtgacg tgggacgagg
     4861 cgatgaactc gagtttccag ttctgcccga acatcgatca actgaccacc gacagccccc
     4921 cgccaatcgt ggcggacgcc cagggccgat accccgtgcc ggtgccggga gcttggacgg
     4981 agctatgaac gcgatcaact caacgccacg cgaagcgaca gccgctcccg gcggagccga
     5041 ggctgcacac aacggcatgc ggcaggcaaa aacgccctca ttccagctcg gttcgctcat
     5101 ggccggctgc acctcactcg cgctcgcctg gatggccggg acaccgggcg cgctggccaa
     5161 atcgactgac gatctggtct atgtcgggac ctacactggg tccaagagca agggcattta
     5221 tgcctttcgc ttcaacgcca aagatggccg ggcagagacg ccgtttctgg cggccgaaac
     5281 ggcccacccc accttcctgg ccctggcgcc gaacaagcgg ttcctctatg ccgttggcga
     5341 gttggggacg ttccagggca agaaagccgg tagcgtcagc gccttcagca tcgaccgcgc
     5401 aacgggtagg ctcgacctcc tcaaccaggt gtcgtccggc ggtcccgggc catgccatct
     5461 tgtccttgat gccacgggca aatgggtgct ggtcgcaaac tatggcggcg ggagcgtggc
     5521 cctgttgccg attcaaggca acggcgcgct tggcgaggcg gtgtcgttcc atcaacacca
     5581 gggatcgagc ttgaacccgc agcgccagga aggcccgcat gcgcatggcg tcacgctcga
     5641 tgccgccaat cgcgtcgcga tcgtgccgga tctcggcctg gaccggctca tgatttaccg
     5701 gctggatgct gtaaacggcc ggttgacacc caatgaccca ccccatgccg cagtcaagcc
     5761 agggtctgga ccgcgccatc tggctttcgc cccagattac aagcacgcct atggcatcaa
     5821 cgaactgtcc tcgactatca ccgtctacgc ctatgacagc gcgggaggat cgctcgctga
     5881 agtgcagact ctctcgaccc tgccggacgg tttcgacggc gataactcga ccgccgagat
     5941 cgaggtgcat ccgaccggga agttcgtcta tggctcgaac cgcgggcacg acagcatcgc
     6001 cgtgttcgcc cgggacctcc agcgcggcac gctcgaacgc gtcgcaaacg tcccgaccca
     6061 gggcaaggcc ccgcgcaact tcgcgatcga tcccaccggc gcatggctct gggcggcaaa
     6121 ccagggatcg gacaaccttg tcctgttccg aatcgatccc gagcgaggtc ttctgcagcc
     6181 cgccggcgtc accgtcgaca tcggctcgcc cgtctgtgtc cagtacgtcc ccttggattg
     6241 acctctgaat caagcccgag ggcaacgcgc gatgtcgttg ccctgatcaa cgcgacgccg
     6301 cccgctcagg ccgtggccga aaggatcgcg cagggcggag ctttgtctct caacgccgtt
     6361 gcctcgtccg cttggccgtt cgtttcggcg ctgctccgga gacaattccc ccaccgcccc
     6421 atcgtggtcg taaccaccga cctcaaagcc caggaaagcc ttcatcagga catccagaca
     6481 tggctgacag cggacgcgcc gacggacccg atccggcccg gcccgtcttg tcgagcactc
     6541 ttctacccgg cctgggaatc cttgccccac cagggacaat taccgcacgc cgacatcatc
     6601 agcgagcgcc tcgagaccct cgtcgccctg gctgaacacc gagaacgatc gaatcgcccc
     6661 cgcaagcgcg atcgtaactc ggagtccgaa tccgcccggt ccctcgaccc gggctccgcc
     6721 tcgtcgctga ccagtggcgg cccagaacac ccgcccgcac cggtggatca aatccctccc
     6781 gctcccttgg tggttacatc ggtcggcgca ctgctccaac gcactttccc atccgattgg
     6841 ctcagggcgc gccatcgagt cctgcggcgc ggagacagtc tggccccgct cgatctgatc
     6901 gaatggctcg aggaccaggc ctatgaaccc gaggcgcaag tcacgcaaaa gggcgaactg
     6961 gccctgcggg gcggcatcgt cgatgtcttc ccgttgacca gcccgtggcc ggtccgactc
     7021 gagttctttg gcgacaccct cgaatcgctc cgactcttcg acccgcagac gcaaatctcg
     7081 cgcgaggaaa tcgaacagat cacgctggcc cccgccggcg agatcggcct cctgcgtcaa
     7141 ctcctggacg cctctgcgca tgtcgcgagc gcgcgcgatg cgaacgccga cgccctgcct
     7201 gccgggccca ctgcaccggc tcagccacaa cgagtatccg ccggatccga tcatccagcc
     7261 gcccccttcc cggtgtctga ggcgcgtctc gcttctttcc tggatcacct tggccctgac
     7321 gccctggtgg tgttctgcga tccgatcgcc ctggacgaac aggccgagcg ctatgccgaa
     7381 cagatcccgc ccggggaccc tttccacgta aattggcacc aggtccaagc agatatcgag
     7441 gcccgcgctc tcacccgcat cgctctgttc gagaccgagt ttgggcaggg cgccgcgcct
     7501 gacgatcgtt tgcccggctg gacgggcccc gcactcgatc cgacgactcg cgatccgaac
     7561 caggcgaaca tgaccttcac atcgctcgat gtctatcgtc ccctgggcga tcgccaacct
     7621 gacccggagg tcgccgaggc ccagcgacgc cagttcttca ctcagctcca ccgatggttg
     7681 cggcagggtt ttgcggtcga ggttttctgc aacaacacag gcgaacagca acgactctcc
     7741 gaactctgcc gtgagtctgg actcgccgaa ctcgagaccc gcctgggaac gctctcccgc
     7801 ggtttcctct gcgacgccgc acgggtcgtc gtcgtcaccg acgccgaaat cttcggccgg
     7861 tacaaggtcc agcgcccacg ccggcttaaa tcgccccacg ccgtcgccat gcgttcggcc
     7921 tttgacattg acttcaccga tttggccgaa ggcgattacg tggttcactt gcagtacggg
     7981 attggccgct atctgggcct gcaacgcctg ccgtccggtc gcggacgcaa aggggcgaca
     8041 acgaatcttg aagagccagg ggacggatcg ggcggcgagg aatgcctggt gatcgagtat
     8101 gcgccgggcg gtccgaagga agatgcgccc aagctgtatg tcccggtgac cgagtctcac
     8161 ctggtcagca agtacgtcgg cgcggggcgg gcgcgtcctc cgctcaacac tttgagtgga
     8221 agccattggc tgaaggccaa ggcccaggcc aatgcggccg tccgcgattt tgccgccgaa
     8281 ctgctccgtc tccaggccgc ccggtccgtg cagtcaggat tcgcctttcc gccggacgcg
     8341 ccctggcagc tcgagttcga gagcgcgttc gtctacgagg aaaccccaga tcaactgcgc
     8401 gccatcgagg cgacaaaaca ggatttagaa cgtccgcgcc cgatggaccg cctcatttgc
     8461 ggagatgtcg gcttcggcaa gacggaggtc gccatccgag ccgccttcaa ggtggtgctc
     8521 gcgggccggc aggtcgccgt tctggttccc accaccgtcc tcgcgcacca gcattacaac
     8581 accttccgcg agcggatggc cggttaccca atacgaatcg aactgctctc ccgatttcgc
     8641 acgcgccgcc agcagaaccg catcgtccgt gacctcgccg tcggaggcgt cgatattgtc
     8701 atcggcaccc accggctggt tcaacccgac gtcgtgttca aggacctcgg gctggtggtg
     8761 atcgacgaag agcagcgttt tggcgtggcg cacaaggaac ggctcaagcg gcttcgccaa
     8821 atggtcgacg tgctcaccct gagcgcgacg cccatcccgc gtacgctcta cctggcgctc
     8881 atgggcgccc gcgacatgag cacgatcgag acaccgcccc aggaccgcct ccccgtcgag
     8941 accgtcatcg ccccgtatga cgagcgcctg attcgcgacg ccatccggcg cgagttgaat
     9001 cgcggcggcc aggtcttcta cctgcacaat cgcattcttg atatcgaggc cgtcgcgaag
     9061 cggttgggtc agcttgtccc ccaggcccgc ctggctgtcg gccacggcca gatgcacgcc
     9121 gacgccctcg aagaggtgat gacccaattt gtcaacggcg agatcgatgt ccttctgtcc
     9181 accactatca tcgaaagcgg catcgacatc cccaacgcga acaccatcat catcgatcgc
     9241 gccgaccggt tcggcctggg cgacctctac caactccgcg ggcgtgtcgg acgctacaag
     9301 catcaggcct acgcctacct cctgatcccg cgtcatgccg gtttgctcgc tgacgcccgc
     9361 aaacgcatca gcgccgtgcg ccaattctcg tcccccggca gtggattcaa aatcgccatg
     9421 cgcgacctcg aaatccgcgg cgccggcaat ctgcttggtc ccgagcagag cggccacatc
     9481 accgccgtcg gattcgaact ctactgccgt ctcctcaaac acagcattgc agcgctcaat
     9541 ggcgagccgc tcaagccgct caggaccgtc tccgtgcgct tcgatttcct cgatctcagc
     9601 cccgtcgggc atgaggaatc ggaccgcgcg gcccccgtct cgcctgcgac gcatgaggcg
     9661 ccgcaacccg acgccccgcc tccggctcga atggacgcga aagccaaaac gacgcccccc
     9721 gccccgcccc gcgcggctcg cggctctggc tctgcctcgg cgtgtctgcc cctccgctac
     9781 gtgcctgagc cgcaccatcg catcgagatg taccgcaagc tggcgcaggt gaccgacaag
     9841 gccggactcg atgcggtgcg atccgaactg cgcgaccggt tcggaccccc gcctcccgct
     9901 gtcgaactcc tcgtgcaggt gtctgcgctc aagattctcg ccgcggatcg cggactcgac
     9961 gcgatggaga cacgcgacga ccggttgatg ctcacacgca acggccagtt catccaggtg
    10021 ggcgggcggt tcccgcgcct gaccaaaaag accgccgcgg cgcgattgaa cgagattcgc
    10081 aagatcctcc tggcgctcga accttgccca ctcggcaggg tcagccctta agttgttggt
    10141 ttactggctg ccccacttcg cagagaaccg atgcccgagc agaacgaacc tcgccgcgag
    10201 aacgaattat taacttgagg gctgaccctg cggccaggcc acgccgggac agagaaaacc
    10261 agggtttggt cgcgcaggaa aacgcagtcg caaaccgaca ctctgttgtc ttggtccctc
    10321 cctccagccc ggcgcggttt tgcttctcgc gcccgcatag cagtccactt gcgaagtccc
    10381 agtgccctgt gcaccatcgg ggtgtgaaat atgcgggcta gcgcgggacc agccaggtgc
    10441 ccgcgaaccg atcctggagg ttgcgctgcg gatgcgccag cgagagagcg gtcagtccaa
    10501 tcgccagcag gctcgacagc accgcggccc caaacgggcc aagcggccct ctgagcatcc
    10561 caaacagcag tggcgcgagc aggacgggtg accaggccat caggccacgc cagaagaccc
    10621 gcaggcgcga ggccggcgcg ccgtcgtggc gcacgatggc cacgcccaac gcccttaaca
    10681 cgagtcctcc gcgaaacgcc agcgcgcaca ccagcgcggg cagtccgaca tagacacaca
    10741 aaccaacgag aaacaccatg aacggaaacc accgctcctc gaga
//
LOCUS       DAOQRO010000080        10588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5534_length_10588_cov_5.534606, whole genome shotgun sequence.
ACCESSION   DAOQRO010000080 DAOQRO010000000
VERSION     DAOQRO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5534_length_10588_cov_5.534606"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKK20_04560"
     CDS             complement(<1..232)
                     /locus_tag="PKK20_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99190.1"
                     /translation="MTGALVGLVVWIAAAADDRVVVISPHNTSICSEFGQAFQAWHRE
                     TYGEPATVEWRNLGGPADALKFVQSEFAAKPDG"
     gene            complement(330..1046)
                     /locus_tag="PKK20_04565"
     CDS             complement(330..1046)
                     /locus_tag="PKK20_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNU99191.1"
                     /translation="MFFGPYDWLIIPGLLLGLYAQFRLTSTYNRYVRQPLESGVTGAQ
                     AARVILDRAGLQDVDIHPVQGHLTDHYDPLRRALFLSGENYQGSSIAAVGVAAHEAGH
                     ALQHQAAYGPLQLRMMLVPATNFATKAAFLILGAGMLLTYIGLISVKLFAALLPFAIG
                     AYAVVTFFQLVTLPVEYDASRRAKDQLLRHGLVSGRERAALSRVLNAAALTYVAGLIT
                     AVLEMLRIILIARSLGDNRG"
     gene            complement(1365..2492)
                     /locus_tag="PKK20_04570"
     CDS             complement(1365..2492)
                     /locus_tag="PKK20_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNU99192.1"
                     /translation="MSEDNLLLVADSERDANMLYAVKMFIPDPFVYLRREGRSYMIAG
                     DLELNRARREANGCRVLSLSRCQRKLKRPRDESLARVVWLLAHERGIDKFLVPENFPY
                     GLARELRRLKIRLKVRRGALFFPEREFKTADEVKKVSAALMMAEVGMAEAIQALRGAR
                     VGPGGKLAYRGASLTSERLRTIAEVAIVQAGGSASHTIVAGGHRACDPHDPGHGPLRA
                     NQPIIIDVFPRSQKTGYHGDITRTVVKGRASEGLRQLYHAVKSAQQLALRMMRPGRKC
                     CEIHRAVARQFEREGYRTGRRNGRLEGFFHGTGHGVGLELHEPPQITEASDAVLRPGH
                     VVTVEPGLYYASIGGVRIEDMVVITDAGAHNLTKFEKVLEV"
     gene            complement(2684..6154)
                     /locus_tag="PKK20_04575"
     CDS             complement(2684..6154)
                     /locus_tag="PKK20_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99193.1"
                     /translation="MKSLTRLLPSGGSALFCLVAGLVFAPPGAARILDDFNDNAKTGW
                     TDFTFIPGFGLPVEQGGQFQFDQPPANRDIFSASQKTSELFELKEGRTIEFRVDLVEA
                     TGEDAFAVLAFIPNTGGNNPGTLAGYGLAKDPTDVLITKGIQKYFVADDGVTAELKND
                     NVTLVLRLAAKDGNVTITGKVLDKDAGDAVLWERTVVDTPAADIMEGGTDAPPAPFLT
                     TGYFTLYCYQQYSGDKMYSVHYDNAEVFVTEETLLDDFDDNTKTDWADFTFLPGFGIP
                     TETNQQFHFDLPPANRDIFTASQKTSRVIELKDGEQVRLEVDLVEDSGEDAFAVLAFI
                     PNTGGNSPGTLYGYGLAKDPTDVLITKGVQKYFIADDGVTAELKNQNVTLALTLTVHN
                     GNVEACGQVLDKDDNNAVLWSRTVLDTPDADLMQNGTDDPPAPIITTGYFTLYCYQQF
                     SGEKLYSVHYDNAVLHAPPAAGNTAAILSDIAPVEYANFLPASTQIAFKVTDDKPLEN
                     EKISVTLNGAVFTTANGLSVTGTGNSRTVTLGGLAANVNYAAVLSVEDSDGAQTSRNL
                     FFDTFAVDSFVIEVEDYNFAYGQFIDNPVVVGEGGYDPDGYSLQAGVPTVDFTDTRTS
                     PNFTDTKYRPEDPIRMAHALDHERAKYTAAGGAAMGVYDYAVGDIAAGEWMNYTRTFP
                     AGHYEVYLRQGLANMATGESVLEEVTGDPTQPDAAVTPLGSFLGVRTGFQFRNFALTD
                     GTGQNKIVLSLSGKKTLRLRQVTPDPRDGARYLNYLVFVPTGGPSEDRASITSLSPAP
                     GSELNTALPVIDAVIQNRTTSVNVGTVILEVNGQVVSATVTPTPTGATVHYPIAPPPP
                     SGVVNSVKIAFKDSEGVDIAAEWQFTVNYVSLDPANRQPGPGKDRGFYVRVVQADPAY
                     GPYDNSLERAELQLAPNSTIPAILDKSVTEQLIEMSQDGGPAGFFQKEYVVPGLDETG
                     LTDDFAVEANAWLELVAGAYRFTVISDDGFKVSSGASLSAKTPVLGARNGGTANEVTG
                     TFEFYVPVSGLYPFRLVWYERGGNAHVEWSAVNMASGVRSLINDAEVQGAIKAYLDVV
                     PAPAVKVQSSTTVAGGYADDAAAVIDTNAKRITLPLSGGTRFYRLVGASALTITSVQV
                     QGANLIMTYQ"
     gene            complement(6558..7208)
                     /locus_tag="PKK20_04580"
     CDS             complement(6558..7208)
                     /locus_tag="PKK20_04580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU99194.1"
                     /translation="MNDTQIESGFRIRPCLPTDLEAAYDVCLKTGDSGADGTHLYQDD
                     PAALGNLYVGPYIVLEPDLAFVLEDGAGVCGYVLAALDSARFYERLQAEWLPSLCARH
                     PDPEGDPATWTPSQRLYHEYHHYQVYYPPSFRAYPSHLHIDLAPRAQGRGQGMRMMHH
                     QLETLERRGSAGVHLCMSALNHRAYRFYRKLDFDELARIGAAGDEVIYLGRRMDRR"
     gene            complement(7219..8586)
                     /locus_tag="PKK20_04585"
     CDS             complement(7219..8586)
                     /locus_tag="PKK20_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HNU99195.1"
                     /translation="MSLARTGAGSPLNGVVEGFYGRPWTLRERLALIDWLHGAGLNAY
                     LYAPKDDLKHRVLWREPYTRSEAAGLRAVADACRERGVQFIYAIAPGLDLAAAKPKET
                     ATLIAKLDQVRRLGASHCAILWDDLPAGLSQEDRRLYGTVAAVHCAVANRVADALEPN
                     PASCRLLFCPTVYCGRMAEPSVRQSPYLREIGEKLRPSIEFLWTGPHIVSETIPTNSI
                     RALRQIVGRKPILWDNLHANDYDSRRLFMGPYAGRAPALRQEIGGVLLNPNSQFDANF
                     IPIRTLGDWCRSAHPPAARTAFRRATAAWLPAFTCRGGATFEIDDIGLLADFFYLPCE
                     LGNRAKAYLADLKRLLGAAPDRWGTVERRFEEASRRILALCDKLTQLDDRDLLYALYP
                     RMWELKETVLLLLAWIAWRRRAPNPKARFRSPDFRPGVFRGGFAAIVERLLPMDDAGF
                     FTPAV"
     gene            complement(8583..>10588)
                     /locus_tag="PKK20_04590"
     CDS             complement(8583..>10588)
                     /locus_tag="PKK20_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNU99196.1"
                     /translation="LEDPFLYEVDAVLSPAGSQHRVSTYFGMRKISVIDLPGTDFPYI
                     ALNDRPVYLQMTLDQAYHPEGFYTYPSDEFMRDEILRSRRIGLNANRLHVKIDVPRKL
                     YWADRLGLLLMCDVPNSWGEPDAEMRREIEHALRGMIQRDFNHPSIFSWVPFNETWGL
                     FTKQGDKRVYLPETQEWVASVYRLAKALDPTRLVEDNSPCNYDHVETDINSWHAYLPG
                     YAWRESLAQVTRDTYPGSVWNFIGGRKQARQPLLNSECGNVWGYEGSTGDVDWSWDYH
                     IMMNEFRRHPKICGWLYTEHHDVINEWNGYYRYDRSEKITGLEDLVRGMSLNDLHGPF
                     YISIGSDLCRDVKPGEVVEVPLWASFMADHAPGQALQINVQTFGWDNLGQNWTLGNEV
                     RPVPFRPWMSEALAPLKLNMPGADGLLIVALRLETATGLPLHRNFTTFRVTNGPAPRD
                     EIRQVQSASVRVVRFAPQTFSAAQWSLKQWNILDGLKANGAGHGFFEYRVAWPAGLSP
                     AKVGGVSLLLEASAKQLLGKDREGSGQQDGDYMRGKGTLDPSLNPNAYPMTDMVRFPS
                     AVRMRVNGVAAGLFDLPDDPADHRGILSWHAQLRDRKLREAGSYGYLIRADLPASSVR
                     AAAESREFVIRLEVDAGLPGGLAIYGERFGRYPVDPSLVFELK"
BASE COUNT         1983 a         3217 c         3458 g         1930 t
ORIGIN      
        1 tcccgtcggg cttggcggca aactcggatt gcacgaattt gagggcgtcc gccgggccgc
       61 ccaggttccg ccactcgacg gtggcaggct cgccataggt ttcgcggtgc caggcctgga
      121 aagcctgacc gaattcggag cagatcgaag tgttgtgggg cgagatgaca accacccgat
      181 cgtccgctgc ggcagcaatc cacaccacca gcccgacgag ggcgcctgtc attccccgag
      241 ggcaggcacg agcccgaatc aaccatgaag acgtgggcgc agtgtctttc actccacaag
      301 cccgccccga acgggtctcg acgcatctgt cagccccggt tgtcgcccag gctgcgggcg
      361 atcaggatga tacgcaacat ttcgagcaca gcagtgatca gaccggcgac gtacgtgagg
      421 gcagccgcgt tgagcacgcg gctcagggcg gcgcgttcgc gcccgctgac aagcccgtgg
      481 cgcagcaact ggtccttcgc gcgccgactg gcgtcgtact cgacgggcag ggtgacgagc
      541 tgaaagaacg tgacgaccgc gtaggccccg atagcgaacg ggaggagcgc ggcgaacagc
      601 ttgacgctga tgaggccgat ataggtcagc agcatgcccg cacccagaat caagaaagcg
      661 gccttcgtcg cgaaattggt ggcgggcacg agcatcatcc gcaactgaag cggcccatac
      721 gcggcttgat gctggagggc atggcctgcc tcgtgagcag ccacccccac ggccgcaatc
      781 gaactgccct ggtaattctc cccggaaagg aagagcgcgc gcctgagggg atcgtaatgg
      841 tcggtcaagt gcccctgcac ggggtgaatg tcgacatcct gaagaccggc gcggtcgaga
      901 atgactcggg cagcctgggc cccagtcaca cccgactcga gaggttgacg gacatagcgg
      961 ttgtacgttg aggtaagtct gaactgggca tagaggccca gaagcaaacc cgggatgatc
     1021 agccaatcgt atggtccaaa aaacataatc gtgccttcga gtcgcggcct ggctccctcc
     1081 gcggtctcgc gaaaaagccc cgcccgcatc cgcctgcatc tgaggttgag agaggagcag
     1141 ccttctttgc atggagcggc acctcagacg ccacgctggc aacagatgtc tcaaaaaacg
     1201 gcgaagcgca aggccgaatg ctcagcagtt ctcatttcgg cgtgggcctc cgacgagaac
     1261 cggctgaagc cgggacactc caccgcgtcc gacgcttcgg cgcggtgttc ggtgtgtcgc
     1321 cttcaggcgg ctcgtgacca aaatgagaac cgctgccgaa ggcttcacac ttcgagaacc
     1381 ttctcgaact tggtgaggtt gtgggcgccg gcgtccgtga tcacgaccat gtcctcgatc
     1441 cggacccctc cgattgaagc gtagtagagc cccggttcga cggtaacgac gtggccgggg
     1501 cggaggacgg catcactggc ttcagtgatc tggggaggct cgtggagttc gagtccgacg
     1561 ccatgacccg tcccgtgaaa gaatccctcc aaccgtccat tgcggcggcc ggtgcggtat
     1621 ccctcccgct cgaactggcg cgcgaccgcg cggtgaatct cgcagcactt gcggccggga
     1681 cgcatcatcc gcagggcaag ttgctgggcg cttttgacag cgtgataaag ctgtcgaagg
     1741 ccttcggagg ctcggccttt gacgacggtg cgcgtaatat ctccgtggta cccggtcttt
     1801 tgggagcggg gaaagacatc gatgatgatg ggctggttgg cgcggagggg gccgtggccc
     1861 gggtcgtggg gatcgcaggc tctgtgtccg ccagcgacga tggtgtggct ggcgctgccg
     1921 cccgcctgga caatggcgac ttcggcgatg gtgcgcagac gttcggaggt cagagaggcg
     1981 ccgcggtagg cgagtttgcc cccggggccg accctggcgc ctcgcagggc ctgaatggcc
     2041 tcggccatcc cgacctcggc catcatgaga gccgcgctga cctttttaac ctcgtcagcg
     2101 gtcttgaact cgcgttcggg aaaaaagagc gcaccgcgcc gaactttcag tcgaatcttc
     2161 aaccggcgga gttcgcgggc caggccatac gggaagttct ccggcacgag aaacttgtcg
     2221 attcctcgtt cgtgtgcaag cagccacacg acgcgggcga ggctctcgtc acggggacgt
     2281 ttcaacttgc gctgacagcg gctcaaggag agaacgcggc aaccgtttgc ctcgcggcgg
     2341 gcgcgattca gttcgagatc gccggcgatc atgtagcttc gtccctcgcg gcgaaggtag
     2401 acgaaaggat ccgggatgaa catcttgacc gcatacagca tgtttgcgtc ccgctcgctg
     2461 tcggcgacca gcagcaagtt gtcctcgctc attgctgcgg ctctggggtc ggcgcggagg
     2521 tcgtggtggt gggcattgtc atgggggctg tttggctttc caaagcgagt ggacgcggcg
     2581 ggatggggcc cgccgcgtcc gcgagtctag taccgatatt cgaacgttcc cggccgccgg
     2641 gttcctttca tttccaggga aagaaacgcg cggcggcaat cggtcattgg tacgtcatga
     2701 tcaggttcgc gccttgcacc tgcacggagg tgatcgtcaa cgcagaggcg ccgacgagcc
     2761 gatagaatcg cgtgccgccg ctcaagggga gcgtgatgcg cttcgcgttt gtatcgatga
     2821 cagcggcggc atcgtcggcg tagcccccgg cgacggtggt cgacgactgc accttgacag
     2881 ccggggccgg aacgacatcg aggtaggcct taatggcgcc ctggacctcc gcgtcgttga
     2941 tcagggagcg aaccccactg gccatgttca ccgcgctcca ttcgacatgc gcgttgccgc
     3001 cacgctcgta ccagaccaga cggaacgggt agagacccga gaccggcaca tagaactcaa
     3061 acgtgcccgt cacttcgttg gcggttccgc cgttgcgggc gccgaggacg ggggtcttgg
     3121 cgctgagact ggcgccggac gagactttga agccgtcgtc actgatcacg gtgaagcggt
     3181 aggcaccggc taccaactcg agccaggcgt ttgcctcgac ggcgaagtcg tccgtcagac
     3241 cggtctcgtc cagacctggc accacgtact ccttttggaa gaaaccggcg ggcccgccgt
     3301 cctgggacat ctcgatcaac tgctcagtga cgctcttgtc gagaatggct gggatcgtcg
     3361 aattgggcgc cagttggagt tcagcccgtt cgaggctgtt gtcgtacggt ccatacgcag
     3421 gatcggcttg gaccacccgg acgtagaagc cgcgatcctt cccggggccg ggctgacggt
     3481 tggcgggatc gaggctcacg tagttgacgg tgaactgcca ctccgcggct atatcgacac
     3541 cctcgctgtc cttgaacgcg atcttgacgc tgttgaccac gcctgaggga ggaggaggcg
     3601 cgatggggta atgcaccgtc gcgcccgtag gcgtcggtgt cacggtggcg gagacgacct
     3661 ggccgttcac ttccagtatc acagtgccga cgttgacgga cgtggtgcgg ttctgaatca
     3721 ccgcgtcaat caccgggagc gcggtgttca actccgagcc tggcgcgggc gagagggagg
     3781 tgatggacgc ccgatcctcc gacggcccgc cggtaggaac aaagaccaag taattgaggt
     3841 agcgagcccc atcacgggga tccggcgtga cctggcgcaa ccgcaaggtc ttcttgcccg
     3901 agagtgagag gacgatcttg ttttgccccg tgccatcggt gagcgcgaag ttgcggaact
     3961 ggaatccggt tcgcacgcca aggaacgagc cgagcggcgt gacggcggca tccggctggg
     4021 tgggatcgcc agtgacttct tcgaggacac tttcgccggt tgccatgttg gcgaggccct
     4081 ggcggagata gacctcgtaa tggcccgcgg ggaaagtgcg cgtgtagttc atccactcgc
     4141 cggcggcgat gtcgccgaca gcgtagtcat acacgcccat ggccgcgccg cccgcggccg
     4201 tgtatttggc ccgctcgtga tcgagggcat gcgccatgcg gatggggtcc tcgggccgat
     4261 acttggtgtc cgtgaaattg ggcgaggtgc gggtgtctgt gaaatccact gtcggcactc
     4321 ctgcttgcaa gctatatcca tccggatcat agccgccctc cccgacgacg acgggattgt
     4381 cgatgaactg gccgtaggcg aaattgtagt cctcaacttc gatgacaaag ctgtcgaccg
     4441 cgaaggtatc gaagaagagg ttgcgcgagg tctgggcgcc gtcgctgtct tcgacggaga
     4501 ggacggccgc gtagttcacg tttgcagcca ggccacccag ggtgacagtg cggctgttgc
     4561 ccgtcccagt gacgctgaga ccgttggcgg tggtgaagac ggcgccgttg agcgtgaccg
     4621 agattttctc gttttcgagt ggtttatcgt cggtcacttt gaacgcgatc tgggtcgagg
     4681 ccggcaggaa gttggcgtat tcgacgggcg cgatgtccga gagaatggcc gcggtgttgc
     4741 cggcggcggg cggcgcgtgg aggacggcat tgtcatagtg caccgaatag agcttctcgc
     4801 cgctgaactg ctggtagcag tacaaggtga agtagccggt ggtgatgatg ggcgcagggg
     4861 gatcatcggt gccattctgc attagatcgg catcaggcgt gtccagaacg gtccgtgacc
     4921 agagcacggc attgttgtcg tccttgtcca gaacctgacc gcaggcctcg acattgccgt
     4981 tgtgcaccgt gagggtgagc gccagggtga cgttttggtt cttcagttcg gcggtgacgc
     5041 cgtcatccgc gatgaagtac ttctgaactc ccttggtgat gagcacgtcc gttggatcct
     5101 tggcgagacc gtatccatac aatgtgccgg ggctgttgcc gccggtgttc ggaatgaacg
     5161 ccaggaccgc gaacgcgtcc tcgccggagt cctccaccag gtcgacttcc agacgcacct
     5221 gctcgccatc cttgagttcg atcacgcggc tggtcttctg gctggcggtg aagatgtccc
     5281 ggttggccgg cggcaagtca aagtggaact gctggtttgt ctcggtggga atcccgaaac
     5341 cgggaaggaa cgtgaaatcc gcccagtcgg tcttggtgtt gtcgtcaaaa tcgtcgagca
     5401 gtgtctcctc cgtgacaaag acttcggcgt tgtcgtaatg gaccgagtac atcttgtccc
     5461 cgctgtattg ctgatagcag tacaacgtga aatagccggt ggtcaggaaa ggcgcgggcg
     5521 gcgcatcggt cccgccctcc atgatgtcag ccgccggcgt gtccacgacc gtccgttccc
     5581 acagcacggc gtcgccggcg tccttgtcca ggaccttgcc ggtgatggtg acgtttccgt
     5641 ccttggcggc gagtcgcagg accaacgtga cgttgtcgtt cttcaactcg gcagtgacgc
     5701 cgtcgtccgc aacgaagtac ttctgaatgc ccttggtgat cagcacatcg gtcggatcct
     5761 tggcaaggcc gtatccggcc agggtccccg ggttattgcc cccggtattt gggatgaacg
     5821 ccaacaccgc aaacgcatcc tcgccggtgg cctcgacgag atcgacgcgg aattcgatgg
     5881 ttcggccttc cttgagttcg aacagttcgc tggtcttctg gctcgcgctg aagatgtccc
     5941 ggttggctgg cggttggtcg aattgaaact ggccgccctg ctcgacaggc aggccgaaac
     6001 cagggataaa cgtgaagtcg gtccagccgg tctttgcatt gtcgttgaag tcatcgagga
     6061 tacgggctgc gccaggaggg gcgaacacca acccggcgac aaggcagaac aaggccgagc
     6121 caccgctcgg caacaggcgt gtcagggatt tcataggcgt gtttccgcgg gggttcttgg
     6181 tttgtgtttc tgaatcagcg tccggcttct ctccagcatc caaacgaccg cacaatcggg
     6241 cgcgccgcga ttcacgcctt cgcgtcggtc tggtttggat cactgctccg agtttcaagc
     6301 ccgtggctcc gaacgtcaac gaaattcaag tccgcagcga agggggcagc gccaattcac
     6361 gaagccgcaa aggccggagc ggggacggct catccccgac caacgcgagt gaagacggta
     6421 tttgccggcg acggggccca cgctccggag cgcgatgaga ctcaatcggg aggacggcct
     6481 ccaacccgca gggcggtcat cgtgcgaggt ccgaccgccc tgtgaactga ccgctacgga
     6541 atacgcgggt tcggacctca ccgcctgtcc atccgcctgc ccagataaat cacttcgtcg
     6601 cctgcagccc caatccgggc aagctcgtcg aaatccagct tgcgatagaa ccggtacgcg
     6661 cggtgattca aggcgctcat gcacaggtga acgccggccg aaccgcgccg ctccaacgtc
     6721 tccaattggt ggtgcatcat ccgcatcccc tgaccccggc cctgtgcccg cggcgccaga
     6781 tcgatgtgaa ggtgagatgg gtaggcgcgg aaagacggcg gataatagac ctgatagtgg
     6841 tgatattcgt ggtagagccg ttgagacggc gtccatgtcg ccgggtcgcc ctcgggatcg
     6901 ggatgccggg cgcacaagga cggcagccat tcggcctgca gacgctcgta aaagcgcgcc
     6961 gaatccaggg ccgccagcac ataaccgcag acgcccgcac cgtcttcgag gacaaaggcc
     7021 agatcgggtt cgaggacaat gtacggcccc acgtagagat tccccaaagc cgcgggatcg
     7081 tcctggtaca gatgcgtgcc atcagccccg gagtcgcccg tcttcagaca gacgtcgtac
     7141 gcagcctcga gatccgtcgg aagacatggg cggatgcgga aacctgattc gatctgcgtg
     7201 tcattcatcg gagtgtgctc agactgccgg agtgaaaaat cccgcatcgt ccatcggcag
     7261 aagccgctcg acaatcgccg cgaatccgcc acgaaagacg ccggggcgaa agtcgggcga
     7321 gcgaaaccgc gccttggggt tcggtgcgcg acggcgccag gcgatccagg ccagcagcag
     7381 cagcacggtc tctttgagtt cccacatgcg cggatagaga gcgtagagca aatcgcgatc
     7441 gtcgagctgc gtcagcttgt cgcaaagagc cagaatccgc cggctcgcct cctcgaaacg
     7501 cctctcgact gtgccccacc ggtccggcgc cgcgcccagc aagcgtttca ggtcggccag
     7561 gtaggccttg gcacgattcc ccaactcgca cgggagatag aagaaatccg cgagcaggcc
     7621 gatgtcgtcg atctcgaaag tcgcgcctcc gcgacaggtg aacgcgggca gccaggctgc
     7681 cgtcgccctg cggaaggcgg tgcgcgcggc gggggggtgc gccgaccgac accagtcgcc
     7741 cagggtgcgg atgggtatga agttcgcgtc gaactgggag ttgggattca gcaacacccc
     7801 gccgatctcc tgccgcagtg caggtgcgcg ccctgcgtac ggacccataa acagccgccg
     7861 gctgtcgtaa tcgttggcgt gaagattgtc ccagagaatc ggtttgcgcc caacgatctg
     7921 acgcaacgcg cgaatcgagt tcgtcggaat cgtctcggag acaatgtgcg ggccggtcca
     7981 gaggaactcg atgctcggtc ggagtttctc gccgatctcc cgcaggtagg gcgattggcg
     8041 gacggacggc tcggccatgc gcccgcagta gacggtggga cagaagagca gccggcatga
     8101 ggccggattt ggctcgagcg cgtcggccac gcggttggcc acggcgcaat gaacggcggc
     8161 cacggtgccg tacagccgcc ggtcctcctg ggagagtccg gcgggcagat cgtcccagag
     8221 aatcgcgcag tgggatgcgc cgagccggcg gacctgatcg agtttggcga tgagagtcgc
     8281 agtctctttc ggcttggcgg ccgcgagatc caagcctgga gcgatcgcgt agatgaactg
     8341 caccccgcgt tcgcggcacg cgtcggcaac cgcgcgcaac ccggctgcct ccgatcgcgt
     8401 gtagggctcg cgccagagca cgcggtgctt cagatcgtct ttcggggcgt acagataggc
     8461 attcaagccg gccccgtgta accaatcgat caaggctagc cgctcgcgta gggtccaggg
     8521 ccgaccgtag aagccctcga ccacgccgtt gaggggcgat cctgctcctg tgcgcgcaag
     8581 gctcacttca attcgaacac caggctcgga tccactgggt accgtccgaa ccgctcccca
     8641 taaatggcca acccgcccgg caggccggcg tcgacttcga gtcgaattac gaactcacgg
     8701 ctctccgccg cggcacgcac gctcgaggca ggcagatcgg cgcgaatgag gtacccatac
     8761 gaaccggcct cgcgcagttt cctgtctcgc aactgggcgt gccacgagag gatgccccgg
     8821 tgatcggcgg ggtcatcggg cagatcgaac aggccggccg caaccccgtt gacacgcatc
     8881 cgcacggcgc tcgggaaccg caccatgtct gtcatcggat aagcgttcgg attgagactg
     8941 gggtcgagcg tccctttgcc ccgcatgtaa tcgccgtcct gctgtccgct cccctcgcgg
     9001 tccttgccca acaactgctt ggccgaggct tccaggagca gcgacacacc gccgaccttc
     9061 gcgggcgaga gcccggcggg ccacgccacc cggtactcga agaaaccgtg tccggcaccg
     9121 ttggccttca ggccatcgag gatgttccat tgcttgagcg accactgcgc cgcgctgaag
     9181 gtctgggggg caaaccgcac gacacgaacg gacgccgatt ggacctggcg gatctcatcg
     9241 cgcggagcgg gaccgttggt gacgcggaag gtggtgaaat tgcgatgcag cggcaggccg
     9301 gtcgccgttt cgagccggag cgccacaatc agaagcccgt cggcgcccgg catattcaac
     9361 ttgagcgggg ccagggcctc ggacatccac ggacggaacg ggaccggccg cacctcgttc
     9421 ccgagggtcc agttttggcc caggttgtcc cagccgaaag tctggacatt aatctgcagg
     9481 gcctgccccg gagcgtggtc ggccatgaag gatgcccaga gagggacctc gaccacctcg
     9541 cccggtttga catcgcggca caagtcgctg ccgatcgaga tatagaacgg gccatgaagg
     9601 tcgttgaggc tcatgccgcg caccaggtct tcgaggccgg tgattttctc ggagcgatca
     9661 taccggtagt aaccgttcca ctcgttgatg acgtcgtggt gctccgtgta gagccagccg
     9721 caaatcttcg ggtggcgtcg aaactcattc atcatgatgt gatagtccca gctccaatcg
     9781 acatcgccgg tgctgccctc ataaccccag acgtttccac actcgctgtt gagcaagggc
     9841 tgtcgcgctt gcttgcgtcc gccgatgaag ttccagacgg acccggggta tgtgtctctg
     9901 gtcacttggg ccagcgattc acgccatgca tagccgggca ggtaggcatg ccaactgttg
     9961 atgtcggtct cgacatgatc gtagttgcag ggggaattgt cctcgaccag ccgggtcgga
    10021 tccaacgcct tggccaggcg atagaccgag gccacccact cctgggtttc gggcagataa
    10081 acgcgcttgt cgccttgttt cgtgaacagc ccccacgtct cgttgaacgg cacccaggag
    10141 aagatggacg ggtgattgaa atcgcgctgg atcatgccgc gcagggcgtg ctcgatctcc
    10201 cggcgcatct cggcgtccgg ttcgccccaa ctgttgggca cgtcgcacat gagcagcagg
    10261 cccaggcgat cagcccaata cagcttgcgg ggcacgtcga ttttcacgtg cagacggttc
    10321 gcgttcaggc caatccgccg cgaccgcaga atctcgtcgc gcatgaactc gtcgctcgga
    10381 tacgtataga acccctccgg atgataggcc tgatcgagtg tcatctggag atacaccggt
    10441 cggtcgttaa gcgctatgta cggaaaatca gtacccggca ggtcgatgac actgatcttg
    10501 cgcatgccga aataagtcga cacgcggtgc tggctgccgg cggggctcag gaccgcgtcc
    10561 acctcgtaca ggaacggatc ttccagcg
//
LOCUS       DAOQRO010000081        10488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5617_length_10488_cov_5.156139, whole genome shotgun sequence.
ACCESSION   DAOQRO010000081 DAOQRO010000000
VERSION     DAOQRO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5617_length_10488_cov_5.156139"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKK20_04595"
     CDS             complement(<1..512)
                     /locus_tag="PKK20_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99197.1"
                     /translation="MELAHLNIGAVIFDVYNTLLDVGPAPADADDRWRRLWRDVLRLQ
                     PRLTRVEFSVACNRAIARCHEAARARGIQWPEVHWPSIVAEVVPELARLPDHARAEFL
                     LRQIQTGRTIRMTDATARALERLKARPTLFGIASNAQAYTLRELEEALAGHGLGLDLF
                     ERDLSFWSFE"
     gene            complement(494..2059)
                     /locus_tag="PKK20_04600"
     CDS             complement(494..2059)
                     /locus_tag="PKK20_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU99198.1"
                     /translation="MYLIVVHYHLRPGGVRRVIELATPHLVRRAPEPVSAVVLGTGEA
                     PDADWLRGFRRRLGRTPVKVFVEPAFGYVSESNRNRRAIDRAILDGMKRLLAETSENN
                     CLVWAHNLGLGRNLRLARRLVFACHNLGIPLIAHHHDWWFDNRWHHFRAKSESGLSRL
                     DLLANTVLAASPSVCHVAINQADAAVLGKHFPGSSGWLPNPVEPETPPTAARARAAET
                     WLIEQIGGKAPVWLLPCRLLRRKNIAEALLLTRWLRPEAWLATTGGVSSAEEESYAST
                     LNAAARRHGWRLRLGLLQGNETLKPSVPELLAASEAVLLTSLQEGFGLPYLEAAAAGR
                     PLIARQLSNIAPDLETFGFTFPQSYREVQVGPDLFDWRSERQRQAGLFAEWKHLMPRA
                     ARRLAGKPTLLAAGSRRCPVPFSRLTLTAQIEVLARPIDESWARCAPLNPFLETWRTR
                     AAAAQLQISPWPQSAARWLGGDIYAARFWSLVPTGLAARPAPGASAAAQGQFLLQKLK
                     AENLYPLLWNSRT"
     gene            2264..3478
                     /locus_tag="PKK20_04605"
     CDS             2264..3478
                     /locus_tag="PKK20_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016856081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNU99199.1"
                     /translation="MSDFFQTGAVATLHRLGRPNVERLERELERFSEENPIALVLPCH
                     IRELGTPALRLILSELKSVTYIQQIIVGIDGATRPADFERARRFFGQLPQRPTLLWND
                     GPRIQDLYRLVEAAELNLGAPGKGRNVWTCFGYVLASEQARMVAVHDCDITTYSRELL
                     ARLCYPVAHPSLGFDFCKGYYARVSSQLNGRVMRLMFTPLIRALKCILGAHPFLVYMD
                     TFRYPLAGEIALDIDLVRRLRMPHDWALEIGTLAEVFRNAAPRAICQSELCDNYDHKH
                     QELSARDTDRGLNRMAVEIAKSLFRRMAAEGIKLDAGLFDTLLSAYLRQAEDTLRFYG
                     ADSAINGLVFARHEEEKAVAMFVRSIRVAGKAFIDNPLSTPRIPNWNSVRSALPELLN
                     RMVEAVKADNGL"
     gene            3475..5139
                     /locus_tag="PKK20_04610"
     CDS             3475..5139
                     /locus_tag="PKK20_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HNU99200.1"
                     /translation="MKDLTSLLARLYGPSASTVAGRVSDLIERWRPHMPRRSDQRGVP
                     GLGQDAALLITYADQLRRDGEAPLRTLAEFCERRLRSVLTGIHVLPFFPSSSDDGFAV
                     EDYLSVDPALGTWDDITRLAAHFELMFDAVLNHVSAQGRWFKAFLEDDPRYRDFFVTV
                     AGAPDLTRVVRPRALPLLTEFPTAAGPRKVWTTFSADQVDLNYRNPKVLLAVVDTLLF
                     YVARGARFIRLDAVAFLWKQIGTTCLHLPQTHAIIQVLRAVLDEIAPGVLLITETNVP
                     HAENISYFGDGIHEAQLVYNFALPPLVLHSLATGSAQALTRWSQSLEPVSDRVAFFNF
                     LASHDGVGLNPVRGILEEAQIEALVARTRAHGGYISLKAMPDGSKCPYEMNINYLDAL
                     SDPAAGESPELAARKLLCAHGILLSLQGVPGIYFHSLFGSRGDRRGAEASGIARRINR
                     EKLDCAALEAALNDANSLRARVFEGQCALLRRRQAHPAFAPTAPQEVLALDARVFAVR
                     RESVDGSDRVLCLHNVSGEQVRFECGRGGDAIELDGYEVRWRTESR"
     gene            complement(5153..6442)
                     /locus_tag="PKK20_04615"
     CDS             complement(5153..6442)
                     /locus_tag="PKK20_04615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU99201.1"
                     /translation="MSFCILTIGTPRRIGFISTRFQGTDGVTLEARKWADILGRMGHS
                     CYWMAGLLDAPPEASHTAPLAYFNHPEVAAVQAKLFGVARRSREISTQIQSLKDRLKD
                     ELYCFIDKFRIEVLIPENILAIPMHVPLGLAMTEVIAETTLPVIAHHHDFTWERERFT
                     LNAVNDYLQAAFPPLLYGMEHVVINSMAQKELARRLGIPSQVIPNVLDFETPPPGIGD
                     YSRDFRREIDLSDDDWLILQPTRVVQRKGIEHAIELVRRLADPRAKLVISHPAGDEGN
                     AYLAMLKDRIADAGIDVRFIADRVGEVRGLTAEGRKVYTLADIYPHADLVTYPSYYEG
                     FGNAFLEGVYFGKPIVVNTYAVYARDINPLGFKTIEMTQLVTREVVEQTRQILEDKAL
                     RDEWALTNYELGLKYFSYSVARRKLAARLANLFGEGI"
     gene            complement(6643..7989)
                     /locus_tag="PKK20_04620"
     CDS             complement(6643..7989)
                     /locus_tag="PKK20_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99202.1"
                     /translation="MNRRTFVRASARGGALLAAGSALFTVSRTGRGQGAQERVVLALI
                     GAGGRGSQLAGNFQQVAGVEFKYVCDAFLPRAEAGAKTLARNQARAPQAVQDMRQALD
                     DSEVRGVVLATPEQWHGLGTVWACQAGKDVYVEKNTSLRLHEGIAMIEAARKYQRVVQ
                     VGFQNRSAPYALTARDYIRSGKLGTVVLVKVYNLLEGGAWAARPDGPVPAGLDWDLWL
                     GPAPEVPYNPGRHFGWNDWWDYCGGAFSGDASHQLDLTRMVLGDPPAPRSVICAGGRL
                     GWSDAREIPDTMVVTYDYGDFVMTCESGTAMPYMKKFPNEVRYGNQWPHWPQSSCRVE
                     IYGTRQLMYLGRHGCGWQAMEADGKVVAEDKGHFPDKWHQPNFIDCIRSRARPNGDIE
                     QTHQSACLVHLGVASYRVGRKQLFFDEAARRFTNNDDANQLLRPLARGRYRLPETI"
     gene            complement(8040..9011)
                     /locus_tag="PKK20_04625"
     CDS             complement(8040..9011)
                     /locus_tag="PKK20_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNU99203.1"
                     /translation="MHSLRASIGLAPFALIFAIAAPALAADSPFGAEPRTRPAEWRIT
                     YRGRPVMVYSCAPEKYKPYVKELCTVNGDNVLRDAPHDHLHHHALMYGIRVNGLNFWE
                     EAPGSGVQKSVSMAEPEIRVGAGGQPEAVLRQTIHWVEPADAFLPDTPRVALLVEQRT
                     LTLTVDEARQEVALQWTSAFEVGAKTNQVVLTGSTYHGLGIRFLQDLDPVAAHLNAGG
                     KPDLGDNRQDVSVHRWGSVAFDRPGKPATLVLYGDPANARGRANYFSMRTPFAYLSAT
                     QGLDKEPLVYRSGERFQIQYLVLLSSKLLDPGAIEQRGEAWRAGRTR"
     gene            complement(9284..10021)
                     /locus_tag="PKK20_04630"
     CDS             complement(9284..10021)
                     /locus_tag="PKK20_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99204.1"
                     /translation="MLKTLPLILISAILVGCASSQLRVPSWQSQRTLMLKSRGVLPPS
                     DAKPSEQDIAERQKYLDKLEKSHEDINALARKTLGHLKRQANNNSKLTLATGGVGIAS
                     GIAAGALVVASPANAVWVAALTGVGAGALSFQTRAALEGFSRDAVARVYNDTITRMAA
                     ADTQYQASYQELVANKTTWTDAKWDEVAGAAEVSLQKYRSAATLVALPSGTEEVETLK
                     QRNVELTSQLDQLQKQLDKIKKDNNLK"
BASE COUNT         1797 a         3371 c         3378 g         1942 t
ORIGIN      
        1 tgctcgaatg accagaaact caggtcgcgt tcgaacaggt ccagccccag gccgtgcccg
       61 gccagcgcct cctcgagttc gcgcagcgtg taagcctggg cattggaggc gatgccgaac
      121 agggtcgggc gtgccttgag ccgttcgagg gcgcgggcgg ttgcgtcggt catccggatc
      181 gtgcggccgg tctgaatctg gcgaaggaga aactccgcgc gcgcgtggtc gggaagccgg
      241 gccaactcgg gcacgacttc cgccacaatc gatggccagt ggacttcggg ccattgaatc
      301 ccgcgcgcac gcgcagcctc gtggcagcgg gcgatcgccc ggttgcacgc aaccgagaac
      361 tcgactcggg tcaggcgcgg ttgcaggcgc aggacgtcgc gccagagacg gcgccatcga
      421 tcgtcggcat ctgccggggc ggggccgaca tccaggagcg tgttgtagac gtcgaagatg
      481 acggcgccga tgttcaggtg cgcgagttcc acagcagggg atagaggttt tcagctttca
      541 gtttctggag caggaattgc ccctgcgccg cggcgcttgc gccgggggcg ggtcgggcgg
      601 ccaagcccgt tggcacaaga ctccaaaacc gcgcggcata gatgtcgcct cccagccagc
      661 gtgccgcgga ttgcggccac ggactgatct gcaattgcgc ggcggcggcg cgtgtccgcc
      721 atgtttcgag gaacggattg agcggtgcgc agcgcgccca cgattcgtcg atcgggcggg
      781 ccaggacctc gatctgagcg gtcaaggtca gccggctgaa cggcacgggg cagcggcggc
      841 tgccggcggc caggagcgtc ggcttgcccg cgagccggcg cgccgcgcgg ggcatgaggt
      901 gcttccactc ggcgaagagc ccggcctggc gttgccgctc gctgcgccaa tcgaacaaat
      961 ccggacccac ctggacctcg cggtagctct ggggaaatgt gaatccgaag gtctcgaggt
     1021 cgggggcaat gttcgacaat tggcgggcga tgagcgggcg cccggccgcc gccgcttcga
     1081 gatagggcag accgaaaccc tcctggagcg atgtgagcag gacggcttcg ctggcggcga
     1141 gcagttccgg cacggatggt ttcagggttt cgttaccctg gagcaggccc aggcgcaggc
     1201 gccagccatg ccggcgcgcg gctgcattca gggtcgaggc atatgactcc tcttcggccg
     1261 aactcacccc tccggttgtc gccagccacg cttcgggccg cagccagcgc gtgagcagca
     1321 gggcttcggc gatgttcttg cgtcgaagca gccggcacgg aagcagccag acgggcgcct
     1381 tcccgccgat ctgctcgatc agccatgtct cggccgcccg cgcgcgggct gcggtgggcg
     1441 gcgtttcggg ctcgactgga ttgggcagcc aaccgctcga gccggggaag tgtttgccca
     1501 ggacagcggc gtcggcttga ttgatggcga catggcagac gcttggcgac gcggccagaa
     1561 ccgtgttcgc gagcagatcg agtcggctca aaccgctttc ggatttggct ctgaagtgat
     1621 gccagcggtt gtcgaaccac cagtcgtgat ggtgtgcgat caggggaatg ccgaggttat
     1681 ggcaagcgaa gacgaggcgg cgggcaaggc gcaagttgcg gcccaggccg aggttatgcg
     1741 cccagaccag acagttgttc tccgaggttt ccgccagcag tctcttcatg ccgtccagga
     1801 tcgcgcgatc gatcgcgcgc ctgttcctgt tcgattccga cacataaccg aaggccggct
     1861 cgacgaagac cttgaccggc gtgcgaccga gtcgccgccg gaagccgcgc aaccagtcgg
     1921 catcgggcgc ctcgcctgtg ccgagcacca cggcgctgac gggctcgggc gcgcgtcgga
     1981 ccaggtgggg cgtggccaac tcgatcacgc gccggacgcc gcctggccgc agatggtaat
     2041 ggacgacgat caagtacaca gcgcaggcag tgtctcaccg ggtccaaagg gtggcgaaca
     2101 cttttgagac gagagggaag acaacaggga aggcttgacg cgttcgcggc tcgcaggcca
     2161 gcattcgtca agtggggcgc tttgtcgacc tcattcgggc attcggtata ttgggtggcg
     2221 tggaaaggcc ggatgaccga gtgagctttc aggatcaagc actatgtcag actttttcca
     2281 gacgggcgcg gtggcgacgt tgcaccggtt gggcaggccc aatgtcgagc ggctcgaacg
     2341 ggagttggag cggttttccg aagagaatcc gattgccctc gtgctgccct gccacattcg
     2401 cgagttgggc acgccggcgc tgcggctcat cctgagcgaa ctcaagtccg tcacctacat
     2461 ccagcagatc atcgtgggca tcgacggcgc gacgcgcccg gccgatttcg agcgggcgcg
     2521 ccggttcttc gggcagttgc cgcagcggcc gacgctcctg tggaacgacg gcccccgcat
     2581 ccaggacctg taccgcctcg tcgaggcggc ggaactcaat ctcggcgctc cgggcaaagg
     2641 ccgcaatgtc tggacctgtt tcgggtacgt gctcgccagc gaacaggccc gcatggtggc
     2701 cgtgcacgac tgcgacatca ccacctacag ccgcgagctg ctggcgcgtt tgtgttaccc
     2761 cgtggcgcat ccgagtttgg ggttcgactt ctgcaagggc tattacgcgc gtgtgagcag
     2821 ccagctcaat ggacgcgtca tgcgtctgat gtttaccccg ttgatccgcg ccctcaaatg
     2881 catcctgggc gcccatccgt tcctggtcta catggacacc ttccgatacc cgctggccgg
     2941 ggagatcgcg ctcgatatcg acctggtgcg ccgcttgcgc atgccccatg actgggcgct
     3001 cgagatcggg acgctggccg aagtgttccg caacgcggcg ccccgggcca tttgccagtc
     3061 cgaactctgc gacaattacg atcataaaca ccaggagttg tcggcccgcg acacggacag
     3121 ggggctgaac aggatggcgg tcgaaatcgc caagtccctg ttccgccgca tggctgccga
     3181 ggggatcaaa ctcgacgccg gcctgttcga cacgctcctg agcgcctacc tgcgccaggc
     3241 tgaggacacg cttcgcttct acggcgccga ctccgccatc aacggcctgg tctttgcccg
     3301 gcacgaggag gagaaagccg tggccatgtt cgtccgcagc atccgcgtcg ccggaaaggc
     3361 cttcatcgac aatcccctgt ccacaccgcg cattcccaac tggaacagcg tgcggtcggc
     3421 tctgccggag ctgctcaatc gcatggtcga ggccgtcaaa gcggacaacg ggctatgaag
     3481 gacctcacaa gcctcctcgc gcgcctctat ggcccgagcg catcgactgt cgccggacgc
     3541 gtgtccgatc tcatcgagcg ctggcgccca cacatgcccc ggcgctcgga tcagcgcggc
     3601 gtgcccggct tggggcagga cgccgcgctc ctgatcacgt acgcggacca actccgccgc
     3661 gatggcgaag cccccctgcg cacgctggcg gagttctgcg agcgccgtct gcgctccgtg
     3721 ctcaccggca tccacgtgct cccgttcttc ccgtcgtcgt ccgatgacgg cttcgcagtc
     3781 gaggattacc tgtccgtcga tccggctttg gggacctggg acgacatcac ccggctggcc
     3841 gcgcatttcg aactgatgtt cgacgccgtg ctgaatcacg tgtccgccca gggccggtgg
     3901 ttcaaggcct ttctcgagga cgatccccga taccgcgatt tctttgtcac ggtcgcgggc
     3961 gcgcccgatc ttacacgggt ggtgcggccg cgcgcgctgc ctctgctgac cgagttcccg
     4021 acagcggccg gacccaggaa ggtctggacc accttcagcg cggaccaggt ggacttgaac
     4081 taccggaatc cgaaggtatt gctggccgtg gtggacacgc tgctgttcta tgtggctcgg
     4141 ggggcgcggt tcattcgcct cgatgcggtg gcgttcctgt ggaagcagat cggcaccacc
     4201 tgtctgcatc ttccgcagac ccacgcgatc atccaggtgt tgcgggctgt tctggacgag
     4261 attgcgcccg gcgtgctgct gatcaccgag acgaacgtgc cgcacgcgga gaacatctcg
     4321 tatttcggcg acggcattca cgaggcccaa ctggtctata acttcgccct ccctccgctg
     4381 gtcctgcaca gtctggccac cggcagcgcc caagcgctca cccgctggtc gcaatccctc
     4441 gaacccgtgt cggaccgcgt ggccttcttc aacttcttgg cctcgcacga cggcgtcggg
     4501 ttgaacccgg tgcgggggat tctggaggaa gcgcagatcg aggcgctggt tgcccgcacc
     4561 cgggctcatg gaggctacat ttcgctcaag gccatgcccg atggctcgaa gtgtccgtac
     4621 gagatgaaca tcaactacct cgacgcgctg tcggacccgg cggcggggga atcgccggag
     4681 ctggccgcgc gcaaactgct ctgcgcgcac gggattctgc tgagcctgca gggggtcccg
     4741 ggcatctact ttcattcgct tttcggctcg cgcggcgacc gtcgcggggc cgaggccagc
     4801 ggcatcgcgc gtcggatcaa ccgcgagaaa ctcgattgcg cggcgctcga agcggccttg
     4861 aacgacgcga actcgctgcg agcccgtgtt ttcgaggggc aatgcgccct gctgcgccgg
     4921 cggcaagcgc accccgcctt tgcaccgacc gcgccgcagg aggtgctggc gctggatgcg
     4981 cgggtgttcg ccgtgcgccg ggagagtgtc gacggctcgg accgggtgct ctgcctgcac
     5041 aatgtgagcg gggagcaggt gcggttcgag tgcgggcgcg gcggagacgc tatcgagctc
     5101 gatggctacg aggtgcggtg gcgcaccgag tcgagatagt gcgcaaggac ggtcagatgc
     5161 cttcgccgaa gaggttggcc agccgggcgg ccagcttgcg gcgcgcgacc gaatacgaga
     5221 aatacttgag ccccagctcg taattggtca gcgcccattc gtcacgcagc gccttgtctt
     5281 cgagaatctg gcgcgtctgc tcgaccacct cgcgcgtgac aagctgcgtc atctcgattg
     5341 tcttgaagcc gagcgggttg atgtcgcgcg cgtacacggc ataggtgttg acgacgatcg
     5401 gtttgccgaa gtagacccct tcgagaaagg cgttgccgaa gccttcgtag tagctgggat
     5461 aggtcaccag atcggcgtgc ggatagatgt ccgccagcgt gtagaccttc cgcccctccg
     5521 cggtcaaacc gcgcacctca cccacccggt cggcaatgaa ccgcacgtca atccccgcat
     5581 cggcgatccg atccttcagc atcgcaaggt aggcgttgcc ctcgtcgccc gccgggtgcg
     5641 agatgaccag cttggcgcgc gggtcggcga ggcggcggac cagttcgatc gcgtgctcga
     5701 tccccttgcg ttgcaccacg cgcgtaggtt gcaggatgag ccagtcatcg tcgctcagat
     5761 cgatctcgcg ccgaaaatcc cgactgtaat cgccaatccc cggcggcgga gtttcgaaat
     5821 cgaggacgtt gggaatgacc tgggatggga tgcccaggcg gcgggccagc tctttttggg
     5881 ccatcgagtt gatgacgaca tgttccatgc cgtagagcag gggcgggaaa gcggcttgga
     5941 ggtaatcgtt caccgcgttg agggtgaatc gctcgcgttc ccaggtgaaa tcatgatgat
     6001 gggcaatgac cggcagcgtg gtctcggcta tcacctccgt catggccagc ccgagcggca
     6061 catgcatcgg tatcgccaga atgttctccg gaatcagcac ctcgattcgg aacttgtcaa
     6121 tgaagcagta tagttcgtcc ttgagccggt ccttgagcga ttggatttga gtcgaaatct
     6181 cgcggcttcg ccgcgccaca ccgaagagct tggcttgcac cgccgcgact tccgggtgat
     6241 tgaaataggc cagcggcgcc gtgtggctcg cctcgggcgg cgcatcgagc aaccccgcca
     6301 tccagtaaca ggaatgcccc atccgcccga ggatgtccgc ccatttgcgc gcctcgaggg
     6361 tgaccccgtc ggtgccttga aaacgggtcg agatgaagcc gatccgacgc ggcgtgccga
     6421 tggtcagaat gcagaagctc atgattgtcg ttactgtccc aggtattgat ctgcggcgca
     6481 gaccttgtcg cacgaggcgg ctcgcggtca atccgcgctt tcagccggat gcgcagcagt
     6541 gttcactctg cgtgcagggc cttgacgccg ggtgaggcca cccggcatac agcatcgtcg
     6601 gctctcgccc tggagaccgg tttccccgac ccggcgccgc gttcagatgg tctcgggcag
     6661 ccggtagcgc ccgcgggcca gggggcgcag caactggttg gcgtcgtcgt tgttggtgaa
     6721 ccgccgggcc gcctcgtcga aaaagagttg cttgcgtccg acgcgatacg aggccacgcc
     6781 cagatgaacc agacaggcgc tctggtgcgt ctgctcgatg tccccgttgg gacgcgcgcg
     6841 cgaccgaatg cagtcgatga agttcggttg atgccatttg tcggggaaat ggcccttgtc
     6901 ttcggcgaca accttcccgt cggcttccat cgcctgccat ccgcacccgt gccggcccag
     6961 gtacatcaac tgccgtgtgc cgtagatttc gacccggcat gagctctggg gccagtgggg
     7021 ccattggttg ccgtaacgca cctcgtttgg gaacttcttc atgtacggca tcgccgtccc
     7081 gctctcgcag gtcatcacga aatcaccgta gtcgtaggtg acgaccatcg tgtccggaat
     7141 ctcgcgcgcg tcggaccagc cgaggcgtcc gcccgcgcag atcaccgacc tgggggcggg
     7201 cggatccccc aacaccatgc gtgtcaggtc cagttgatgg ctggcgtcgc ccgagaacgc
     7261 cccgccgcag tagtcccacc agtcgttcca gccgaaatgc cggcccggat tgtacggcac
     7321 ctcgggggcc ggcccgagcc acaggtccca gtccagcccg gctgggacgg ggccatcggg
     7381 acgggccgcc caggcgccgc cttcgagcag gttgtagacc ttgaccagga caaccgtgcc
     7441 cagtttgccg ctccgaatgt agtcgcgcgc cgtcagcgcg tagggcgcgc tccgattctg
     7501 gaacccgacc tgcaccacgc gctggtactt gcgcgctgcc tcgatcattg cgatcccctc
     7561 atgcagccgc agcgaggtgt tcttctcgac gtagacgtcc ttgccggcct ggcacgccca
     7621 cacggttccc agcccgtgcc actgttcggg tgtggccagc accacccccc tcacctctga
     7681 atcgtccagc gcctggcgca tgtcctgcac cgcctggggc gcacgcgcct gattcctggc
     7741 cagggtcttc gctcccgcct cggctcgcgg caggaaggcg tcgcagacgt acttgaactc
     7801 gaccccggcc acttgttgga agttgccggc gagctgcgag ccgcgtccgc ctgcgccgat
     7861 caacgccagg acgacgcgct cctgcgcgcc ctggccgcgg ccggtccggc tgactgtgaa
     7921 cagagcgctt cccgcggcca gcagggcgcc gccgcgtgcg gatgcgcgga cgaaggtgcg
     7981 acgattcatt ggtttcatcg atatgccccc tttccagagg cgctgcctga tgcggtcgtt
     8041 cagcgtgtgc gtccggcgcg ccaggcctcg ccgcgctgtt cgatcgcgcc gggatcgagc
     8101 agcttcgatg acagcagcac caggtattga atctggaatc tctcgccgct gcggtacacc
     8161 agcggctcct tgtcgagacc ctgggtcgcg gacagatagg caaatggtgt ccgcatgctg
     8221 aagtagttgg cccggccgcg cgcattggcg gggtccccat acaagaccag agttgcaggc
     8281 ttcccgggcc ggtcgaaggc gaccgatccc cagcgatgaa cactgacgtc ctgacggttg
     8341 tcgcccaagt cgggcttgcc tcctgcgttg agatgcgccg ccacaggatc gaggtcctga
     8401 agaaatcgga ttccaaggcc gtggtaggtc gatccggtca gcacaacctg gttcgtcttg
     8461 gcgccgacct cgaacgccga cgtccactgc aacgcgactt cctgtcgagc ctcatcgacc
     8521 gtgagcgtga gcgtgcgctg ttcaaccagc agcgccactc gaggcgtatc gggcaaaaag
     8581 gcatccgccg gctcgaccca atggatggtc tggcgcagca ccgcctccgg ctgcccgccc
     8641 gcgccgaccc gaatctccgg ctcggccatc gacaccgatt tctgaacgcc gcttcccggg
     8701 gcctcttccc agaaattcag gccgttgacg cgaatcccgt acatcagggc gtggtgatgc
     8761 agatggtcat ggggggcgtc gcgcaggaca ttgtccccgt tgaccgtgca gagttccttc
     8821 acatacggct tgtacttctc gggcgcgcat gaatagacca tgacagggcg gccccgatat
     8881 gtgattctcc attcggcggg ccgcgttctt ggctcggcgc caaaaggcga atcggctgcc
     8941 agagccgggg ccgcgatcgc aaaaataagg gcgaacggtg caagtccgat cgaagctcga
     9001 agtgaatgca tgaccccagt caaccaccat ttgcgcccgt tgggaagtct ggagtgccca
     9061 aaatgccacg atggcgaatg caatcaacga ctgaccatca atcgtcactg gattcgcacg
     9121 tgcgaccagt ggaagcatca aaagcaacaa gatggcaaga cgcgcccgca tgacggccaa
     9181 cgacccaacc gagcgacgcg ggcggcccag tgcgtccgca ttggcaactg acgtggccta
     9241 cccgcgttcg ttcgagtgat tttgttaggt cgtcgtcgac aggtcatttc agattgttgt
     9301 ctttctttat cttgtccaat tgcttttgga gctggtcgag ttgcgaggtc aactcaacgt
     9361 ttcgttgttt gagcgtctcg acttcctctg tgccggaagg taatgcaacc aacgtcgctg
     9421 cactacggta cttctgcaaa ctcacctctg ccgctcctgc gacctcatcc catttcgcgt
     9481 ctgtccatgt ggtcttgttg gcaaccaact cttggtagct cgcctggtac tgcgtatcgg
     9541 ctgcagccat cctcgttatc gtatcattgt acactcgagc tacagcgtct cgtgaaaatc
     9601 cttccagagc ggcgcgtgtc tggaaactga gtgccccggc accgacgcca gtaagtgccg
     9661 ccacccagac tgcgtttgca gggctggcca caaccaacgc tccagcggcg atccccgaag
     9721 cgatgccaac tccgccggtc gccaaggtca acttgctatt gttgtttgcc tgcctcttta
     9781 gatgtccaag tgtctttctt gccagagcgt taatgtcttc gtgggacttt tccagcttgt
     9841 ccaagtattt ctgtctctct gcgatgtcct gctcgctggg cttcgcatcc gatggcggca
     9901 ggacacccct actcttcaac atcaatgttc tttggctttg ccaggagggg acacgcagct
     9961 gcgaggaggc gcagccaacc aagatggctg agataaggat aaggggaagg gttttgagca
    10021 tattgttttg ttgttgtctt cgagttcgcg accgccattg gacctaacag tcagcaatgt
    10081 atccgtcccg gcggggtcgc gggcacgcga ttcgtctcgg ccgttctccc cgccgggacg
    10141 gatacattca tgttgaacga agccgcggcc ttcgcccagg aattcacagg gcaacgtaag
    10201 cccgggacca gcctgcgaaa agcaagaaat cacacactcg cacctcggca cgcccacctt
    10261 cgcccttcgg attgtgggcg tcagcgcccc gccacggccg ccctccccgc agggtgcgcc
    10321 ccaagcgcag accagcgccc tgggggcgcg acaccccgcc gccggcgcgc aaagggtctg
    10381 cgcaccccaa gtcccctaaa ggcgtcggtc ccggagtaca ggaatgtgga agagactctg
    10441 gtggtccgcg atgtgccggg cgaggagctt gaactacgaa tatctgga
//
LOCUS       DAOQRO010000082        10444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5658_length_10444_cov_4.709982, whole genome shotgun sequence.
ACCESSION   DAOQRO010000082 DAOQRO010000000
VERSION     DAOQRO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5658_length_10444_cov_4.709982"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKK20_04635"
     CDS             <1..856
                     /locus_tag="PKK20_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99205.1"
                     /translation="PPAERIRYLLARRLVRANRCADARRYFPPDWLPLFDRFLVGRLE
                     ADRGDRSTEQRAWGLLEAARMTRRYGLELLGTEVEPDWRIHDGNFEWGVTLATRSTLT
                     TNALGPSLEEIARAQAHRATPERRWHYRHTAAVLAQDAARLLCQSRGPNTTPAQRAEA
                     LMRAARFTVDHNVSWVPGADDDSAPDEPAPPSGDPMALLWNKGYTASALAWEAARLLP
                     DNSDDTAGILCLGGRWIATLDPSVADLFYKALVNRNRRTALGIAADKLRWFPSVDRDG
                     RLLPPSAD"
     gene            914..3007
                     /locus_tag="PKK20_04640"
     CDS             914..3007
                     /locus_tag="PKK20_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNU99206.1"
                     /translation="MKTSASTRLATIAASCLMAIGATGSSDPRPNIVVILADDLGYSD
                     IGCYGGEIQTPNLDRLARDGVRFTQFHNTARCCPTRAALLTGLYPHQAGVGHMTEEQR
                     DSSGRPLAGYSGRLNDRCVTFAEALKAAGYFTAMTGKWHVGQNHGVTPWDRGFDRTLT
                     AAAGGFYFPDSPRAALFLNGKNLGSRGAPLPGEWYSTDLWIEYGLKFVDEARAARKPF
                     FLYVAHNAPHFPLQAPAEDIARWRGKFKAGWDVLRQERYARQLEMGLIDKAWPLSPRP
                     DAVPAWSSLSAEDQDRYDHIMAIYAAVVERMDRSIGTLVSGLRDRGELDNTLILFMSD
                     NGGNAESGVRGRLEGSQPGDAQSTVFVGECWAALNNTPFVRYKHYTDEGGIATPLIAH
                     WPRGISAARNGALEPQPGHVIDIMPTCLEVAGASYPDAFKGRSLTPLEGVSLRPGFEG
                     KPLGRTRPLFWEHEGNRAVLDGKWKLVALAGQAWRLYDTVKDRTEQNDLAASEPDRVK
                     ALAARWDEYAARANVLPLGGWRSSAQNSRGNPAPDLSNETQFALKMGDRLDRAHAPAI
                     AGRPFTITATFESDNPQGVIVAQGGSARGYALFVENGKLVFLVRTAGEVTQVMAPQLT
                     KGAHTAVARLGRGGALSLALDGKPAASGQAPSLIATMPVDGLEVGSDQAGLVGPYSTD
                     NQFRGRIESVRIVLD"
     gene            complement(3474..6566)
                     /locus_tag="PKK20_04645"
     CDS             complement(3474..6566)
                     /locus_tag="PKK20_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99207.1"
                     /translation="MKANNHSNLLQLVASGCAKAACCLAAALALTGSTALEAAITGQW
                     DFKSGDLSATIGLPLEFFDYPGGDTDQQTVFGTTTALGLPDIGGAPAHVMGFPKSLPH
                     MGYVIYPNMHSNGGGAFVNQYTLIFDLLYPPASSTGWRALIQINDPYNTDDADLFINP
                     TGGIGISAQYHGVVQANTWHRLAFAFDLAAPGGPTLSKYIDGALVNRQILGAGVDGRW
                     ALNAVDGMFAPMALLFTDNNEDGADTQAGYVSSIQVHDEALSAAYIQTLGPPTTDGIP
                     TTVVVPASIVSRKPLEGAMNVVPASEIEVVLADGSAPLDTTSIVLKVNNETLTRNVTS
                     AAGKHTVTAALPPLSPRSTNTVSVGFADASAGTVTLEWGFRMAAYLLDAELEQVFTNR
                     LAAYWPMDEGLTNFAATLLVDVAGENHSTITTGGMTDYWLDASESRFGGALHVDGENV
                     YVMVLPSPSLDIGANAMSMALWVKLEQLPSQLPGSYGSIYDSVTDEYVVYTDKGNKEL
                     RFKVTLANGQAARPGIPEAQLKVGEWMHIAAVYDGNASPTAGEARIYLNGELMDRHVG
                     HDGAPGTGLTGNVRAGQTAAIGRPGTATGNNYVGAIDDLAIWSQALSIDAIGYLSSGH
                     SIPAVAQDPDPLTITQHPEDKTGVVGSRVMFEVVRSGGTPPVLYQWKHNGVDIPGATS
                     AKLFVVVAPDAAGTYTVAVRDAVRSLVSNPATLTIVTLSSDPIESLLVGLIAHWRLDD
                     GLADPNTQVLADARGGSPGNLFGGSAAAWLAEPEARFGGALHVDGQTVYATIPNNSIL
                     DINENQVSVSLWARLEQLPTEIPGAFGGIFDSPNDSYVVYLDKGNQELRFKVTTAEGQ
                     AVRPGIPQSELVLNEWIHVLAVYDGNASPDAGEARIYLNGVVMDTEGSASLTGLVRPG
                     QVTGLGRDGETAGYFYTGAIDDIGVWSRALTVDEIAYLSSGHAIPAGPEPLEISGARI
                     ESGQIVIQWTGGEGPYQLQRRTSLTTGNWENAGQPTAGTTASDATDAPVRFYRVIKAQ
                     "
     gene            complement(6606..7256)
                     /locus_tag="PKK20_04650"
     CDS             complement(6606..7256)
                     /locus_tag="PKK20_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU99208.1"
                     /translation="MATVRVLIADDHPIIRSGLREAIEGQRGKWLVFEASDGEQAVEY
                     CRTHPIDVAIFDISMPKLDGLAAVRMTREQKPTLKVILLTMYDDPALLEEGLALGAQG
                     YVLKDEPIVVVLEALRAVLKGERYLSPALQNRDRKRDTVLKPLPDSSPYLELLTVKER
                     KILRLIGEHLTSKEIADRLQCSVHTINAHRQNLSIKLRLSGSHSLLKFAFENRDRL"
     gene            complement(7260..9353)
                     /locus_tag="PKK20_04655"
     CDS             complement(7260..9353)
                     /locus_tag="PKK20_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNU99209.1"
                     /translation="MKKTNFPRPESKRDGNIQRAPIERAPGPSMELTQRKGREACVNR
                     AHDQLEHEVRERTRALEAAIERLRFRENEVRTLVENSQDLIFRFDLKGRHIYANLAAA
                     KLYGKNLDGLRGRRMRDLGTGRETWESARAIFDRVVASGAEAGGQIAHGVGERRRYYD
                     TRFVPEFGAGGVLASILVVARDVTERVRTEEQVLANERLLWAVFNSSFQYMGLLSAEG
                     HVIMINDTALRFAGLKMEDVRGMPFWMTPWWTLSPETQDKVQRAVERAARGETVRYEE
                     VVQGAENQLKTIDFFLRPLDSPAGISPLLIAEAHDITELKRTTDALRDAETLARVQAE
                     TVLEGILTHDCGIIQEANNVVENMFGWSPAELLGKSLIDLIEPESRTLVRQSLVTSAG
                     QRYECLALTRDGQRFPIAIQSRRFPYQGRHVGMVVIHDLTDQQRDRARIRKLEQEEAL
                     KREKAERESFARRLIETQERERCRIATDLHDGLGQNILAIKHHAERCLANGVSPGRTA
                     ESLQEITSLAAQAVREIRAIVQDLHPYLVERLGLTRALQAMVESIAKLSSVHIVSEIQ
                     NIDGVLKPEAEIGLYRILQECLTNVVRHSQATEARLCVAPQQRWVCASVEDTGQGFDL
                     TRIRHGDSASSGLGLRGIVERSALLGGSAWIASHPGGGTRVELQLPIGTAREDSSRGA
                     PLRLVSEPGSGTMSS"
     gene            complement(9527..9766)
                     /locus_tag="PKK20_04660"
     CDS             complement(9527..9766)
                     /locus_tag="PKK20_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99210.1"
                     /translation="MTMFSEPLGTLWLLAAASGAGWMGKAAFIGVFVALIVLLASLPA
                     RMLGETECRPALWRNVRAWAILVAVAQIFIYTLWG"
     gene            complement(9763..>10444)
                     /locus_tag="PKK20_04665"
     CDS             complement(9763..>10444)
                     /locus_tag="PKK20_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99211.1"
                     /translation="FPILLVVPGLVGVALLGPELGQPGKDWDGNRVLPMLVVRLLPPG
                     VLGVVMGAFFAGVISNLDSYVSSASTMVVTDLYRTFIRRQAADRECLLVGRWLVVAFL
                     VGGVAISYPIKKGFGSVFEAFQTFLSFFQGPLLALLLLGMLTRHATPWGGVAGMIIGV
                     STSAVLNSAELLLGVPGGIPFLWTAWWSFVAALAGVVVVSAFTSGHDEARLRGLVCWL
                     PAEGGNKP"
BASE COUNT         1839 a         3355 c         3383 g         1867 t
ORIGIN      
        1 cccgcccgcc gaacgcatcc gctatctcct cgccaggcgc ctggtccgtg ctaaccgctg
       61 cgccgatgcg cgccgttact tcccgccgga ctggctgccg ctctttgacc gcttccttgt
      121 cggacgcctc gaagccgacc gcggcgaccg gtccacggag cagagggctt gggggctgct
      181 cgaagcggcc cggatgaccc ggcgctatgg actcgaattg ctgggcaccg aggtcgaacc
      241 ggactggcgc atccacgatg gcaacttcga atggggcgtt acgcttgcga ctcgcagcac
      301 cctgaccacc aacgcgctgg gccccagcct cgaagaaatc gcacgggctc aggcgcaccg
      361 agccactccc gaacgccgct ggcattaccg ccacacggcg gcggtcctgg cgcaagacgc
      421 agccaggctc ttgtgccaat cgcgtggccc gaacaccacg cccgctcagc gcgccgaagc
      481 gctcatgcgc gccgcgcgtt tcactgtcga tcacaacgtg agctgggttc cgggcgctga
      541 cgacgattcg gcgcccgacg agccggctcc gccttcgggg gatccgatgg ccttgctttg
      601 gaacaaaggc tacaccgcct ctgccctcgc atgggaggcc gcgcgcttgc taccagacaa
      661 ttccgacgac accgcgggca tcctctgcct tgggggaagg tggatcgcga ctctggatcc
      721 gagcgtcgca gacctcttct ataaggccct ggtgaatcga aatcggcgca ccgccttggg
      781 aatcgctgcc gacaagctcc gctggtttcc cagcgtcgac cgggacggac gtctgctccc
      841 cccgagtgcg gattgatcaa tggtcgagca ttgcgcgtcc gcgcctcgat cgcctaccct
      901 gctgcaaatc cacatgaaaa cgagcgcgtc aacacggctt gcgacgattg ccgcgagctg
      961 tctcatggcg atcggtgcga ccggttcgag cgatccgcga ccgaacattg ttgtcatcct
     1021 cgccgatgac ctggggtact ccgacattgg atgctatgga ggcgagattc aaacgcccaa
     1081 cctcgatcgg cttgcccggg acggggtgcg cttcacccag ttccacaaca cggcccggtg
     1141 ttgtccgacc cgcgcggcgc tgctcacagg gctgtatccg caccaggcgg gtgtggggca
     1201 tatgaccgag gagcagcggg attcgagtgg acggccgctg gcggggtaca gcgggcggct
     1261 caacgaccgg tgtgtgacgt ttgcggaggc gctgaaggcg gcgggctatt tcaccgccat
     1321 gacaggcaag tggcacgtgg gccagaacca cggggtcaca ccgtgggacc gggggttcga
     1381 tcggacgctg accgcggcgg ccgggggctt ttactttccg gattcgccgc gcgccgcgct
     1441 gtttctcaac ggcaagaatc tggggtcgcg cggcgcgcct ttgccggggg aatggtactc
     1501 gaccgaccta tggatcgagt acgggctgaa gttcgtcgat gaggcccggg cggcgcggaa
     1561 gcctttcttc ctgtacgtgg cgcacaacgc gccccatttc ccgctgcaag cgcctgccga
     1621 ggacatcgcc cggtggcgcg gcaagttcaa ggccggctgg gacgttctgc gtcaggagcg
     1681 ctacgcgcgc cagctcgaga tggggctgat cgacaaggcc tggccgcttt cgccaaggcc
     1741 cgacgccgtg ccggcctgga gcagcctcag cgccgaggat caggatcgct atgaccacat
     1801 catggccatc tacgcggcgg tggtagaacg gatggaccgg tcgatcggga cactggtgag
     1861 cgggctgcgc gaccgtggcg aattggacaa cacgttgatc cttttcatga gcgacaacgg
     1921 cgggaacgcc gagagcggcg tccgtggacg actcgagggc agccagccgg gcgacgcgca
     1981 gtcgacggtt tttgtcgggg aatgctgggc ggcgttgaac aacacgccgt ttgtccgcta
     2041 caagcattac accgacgagg gcggcatcgc gacccctttg atcgctcact ggccgcgcgg
     2101 catttcagcg gcgcgcaacg gggcactcga gccgcagccc ggacatgtca tcgacatcat
     2161 gccgacctgc ctcgaggtgg ctggggcctc ctatcccgac gcattcaagg gccggtccct
     2221 cacgccgctc gaaggtgtca gcctgcgtcc gggattcgag ggcaagccgc tcggacgcac
     2281 gcgaccgctc ttctgggagc acgaaggcaa ccgggctgtt ctcgacggga aatggaaact
     2341 ggtggcgctg gcgggccagg cttggcgcct gtacgacacg gtcaaggacc ggaccgagca
     2401 gaacgatctg gcggcgtccg agccggaccg agtcaaggcc ctggcggcga ggtgggacga
     2461 atatgcggcc cgagccaatg tcctgccgtt aggcggttgg cggagttcgg cgcagaacag
     2521 tcgtggcaat cctgcccccg acctgagcaa tgaaacgcag ttcgcgttga aaatgggcga
     2581 tcgtctcgat cgcgcccatg caccggccat tgccgggcgc cccttcacga tcaccgccac
     2641 gttcgagtcg gacaacccgc aaggggtgat tgtcgcccaa ggcggctcgg cgcgcggcta
     2701 cgcgctgttt gtcgagaatg ggaaactggt gttcctggtt cggacggctg gcgaagtgac
     2761 gcaggttatg gcgccgcaac tgacgaaagg cgcgcacacg gctgtcgctc gtctcggtcg
     2821 gggtggcgcg ctgagcctcg cgctcgatgg caagcccgca gccagcggcc aggcccccag
     2881 cctcatcgca acgatgcctg tggacggtct cgaggtgggc agcgaccagg ccggcctggt
     2941 cggaccctat tccaccgaca accaattccg cggcaggatc gagtcggtcc gcatcgtgct
     3001 cgattaaaca gagcgacatc gatgatgcag cgccgggtca ggcctgcgag cctgcaggcc
     3061 gcgtggcctc gcgcggcgga acttctgacg ctgtgcatag gagttttcaa acgccgatga
     3121 tggcattcat ctgctggaac tcgatccagc caccgacttg gatcggcgcc agacgatcgc
     3181 gacggcaacg ctgttcccag ggccgtcctg gcgcgcattc tatccgttct cgcatggtgg
     3241 cgccgacgcc agcaaccggt ttgacggcgt gttcgagggc aatgcgacga gtcagaccga
     3301 tccgttcttt cgcggtcaaa tcgacgcgct ggtcctacac caatctcgac cacactgtgt
     3361 cggcgtgccc gacgcgccca aggggctgcg gcgcatcccg actcgaatga accgatgagc
     3421 cgattctcct tgctgggcgc tgaacgatca gagcgggtcc gggggcggca gtgttactgc
     3481 gccttgatga cgcggtagaa tctgaccggg gcatcggtcg cgtctgatgc ggtcgtgccg
     3541 gcggtcggtt gtcccgcgtt ctcccaattc ccagtggtca gagaggtccg gcgctgcaac
     3601 tgatacgggc cttcaccgcc ggtccattgg atgacgatct ggccgctctc gatgcgcgcg
     3661 ccggagattt cgagcggctc cggcccggcc gggattgcat ggcctgagga caggtaggcg
     3721 atctcgtcca ccgtcagcgc gcggctccag acgcctatgt cgtcgatggc accggtatag
     3781 aagtagcctg ccgtctcgcc gtcgcgtccc agaccggtga cttggccggg tcgaaccaat
     3841 ccggtcaggc tggcggagcc ctcggtgtcc atcacgacgc cgttgaggta gatgcgggcc
     3901 tcgccggcat cgggcgaagc attgccgtcg tagactgcga gcacatgaat ccactcgttg
     3961 aggaccagtt cggactgagg gatgccgggc cggacagcct ggccttcggc cgtcgtcacc
     4021 ttgaatcgca gttcctggtt gcctttgtcg aggtagacga catagctgtc attgggcgaa
     4081 tcgaagatgc cgccgaaggc accgggaatc tcggtgggca gttgttcgag gcgcgcccag
     4141 agcgacacgc tcacctggtt ctcgttgatg tccaggatgc tgttgttggg aatcgtcgcg
     4201 tagacagtct ggccgtccac gtgaagcgcg ccgccgaacc gggcctcggg ctcggcgagc
     4261 caggccgccg cgctccctcc gaagaggttg ccagggctgc cgccccgagc atctgccagg
     4321 acctgggtgt tgggatcggc cagtccatcg tccagccgcc aatgggctat gagccccaca
     4381 aggagtgact cgatcggatc gctcgacagc gtgacgatcg tcagggtggc cggattgctc
     4441 acaagcgagc gcacggcatc ccggacggcg acggtgtagg tgcccgcggc gtccggcgcc
     4501 acaaccacga agagcttggc actggttgcg ccgggaatat cgacgccgtt gtgtttccac
     4561 tgatagagca cgggcggagt gccgcccgac cgaacgactt cgaacatcac gcggcttcca
     4621 accacaccgg tcttgtcctc tggatgctgg gtgatggtga gcggatcggg gtcttgagcg
     4681 acagccggga ttgagtggcc cgaggagagg tagccgatcg catcgatgct cagggcctgg
     4741 ctccagatcg cgaggtcgtc gatggctccg acgtagttgt ttccggttgc cgtgccggga
     4801 cgcccgatcg cagccgtctg cccggcgcgc acgttgccag tcaggccggt gcccggggcg
     4861 ccgtcgtggc ccacatgtct gtccatcagt tcgccgttga gatagatgcg cgcctcgccg
     4921 gcggtcgggc tggcgttgcc gtcatagacg gcggcgatgt gcatccactc gcccactttg
     4981 agttgcgcct caggaatgcc gggccgtgcg gcctgtccat tggccagagt aactttgaac
     5041 cggagttcct tgtttccctt gtcggtgtag accacgtact cgtcggtgac cgagtcgtaa
     5101 atcgatccgt agctgccggg caactgactg gggagttgtt cgagcttgac ccagagggcc
     5161 atggacatcg cattggcccc gatatcgagg ctcgggctgg ggagaaccat gacatacacg
     5221 ttctccccgt cgacatgcag agctccgccg aatcgcgatt cggatgcatc gagccagtag
     5281 tctgtcatgc cgcccgtcgt gatggtggaa tggttctcgc ccgccacgtc caccagtaac
     5341 gtcgcggcga agttagtcag gccctcgtcc atcggccagt atgcggccaa ccggttggtg
     5401 aacacctgct cgagttccgc gtcgagtaga taggccgcca tgcggaaacc ccattcgagg
     5461 gtgaccgtgc cggccgaagc atcggcaaac ccgacgctga cggtgttcgt cgaacgcggg
     5521 ctgagcggcg gcagagcggc ggtgacggtg tgtttgcccg cggcggaggt gacgtttcgc
     5581 gtcagtgtct cgttgttgac cttgaggacg atcgaggtgg tgtccagggg cgccgagccg
     5641 tcggccagaa ccacctcgat ttccgaggcg ggaacgacgt tcatggcgcc ttccagcggc
     5701 ttgcgggaga caatcgaggc tgggacgacc actgttgtgg ggatgccgtc ggtggtgggt
     5761 gggcccaagg tctggatgta tgccgcggac agtgcctcgt catggacctg gatgctgctg
     5821 acatagcccg cctgggtgtc tgcgccatcc tcattgttgt cggtgaacaa cagcgccatc
     5881 ggggcgaaca tgccgtcgac cgcgttgagt gcccagcgcc cgtccactcc ggcgccgaga
     5941 atctgccggt tcaccagcgc accatcgatg tacttgctga gtgtcggccc gcccggggcg
     6001 gccagatcga acgcgaatgc gaggcgatgc cacgtattgg cctggacgac gccatgatac
     6061 tgcgccgaga ttccgatgcc tccggtcgga ttgatgaaga ggtccgcgtc gtcggtgttg
     6121 taggggtcgt tgatttggat cagggcgcgc cagcccgtgg aggatgcggg cgggtaaagc
     6181 agatcgaaga tcagagtgta ttggttcacg aatgcaccgc caccgttcga gtgcatgttc
     6241 gggtagatca cgtagcccat gtgaggcagg gacttcggaa agcccatgac atgcgccgga
     6301 gcaccgccga tgtcgggcag acccagggcg gtcgtggtcc cgaagacagt ctgctggtca
     6361 gtgtccccgc ccgggtaatc gaagaactcg agagggaggc cgatggtggc ggagaggtcg
     6421 cccgacttga aatcccactg gcctgtaatg gccgcttcga gcgcggtcga gcccgtcagc
     6481 gccagggccg cggccaggca gcaggccgcc ttcgcgcatc cggacgcaac aagctggagt
     6541 agattcgaat ggttgtttgc tttcataaaa agtcggcatt caattgcttg tgattgcata
     6601 atgtgtcaca gacgatcgcg attctcgaag gcgaacttga ggaggctgtg ggatccgctc
     6661 agccggagtt tgatgctcag attctggcgg tgcgcgttga tggtgtgcac actgcattgg
     6721 agtctgtcgg cgatctcctt gctggtgaga tgctcgccga tcagacgcag aatcttgcgc
     6781 tccttgacgg tcagcaactc gaggtaaggg ctggagtcag gcagcggctt caagaccgtg
     6841 tcgcgtttcc tgtcgcgatt ctgcaaggct ggactgaggt agcgttcgcc cttgagcacg
     6901 gcgcgaaggg cctcgaggac gacgacgatg ggctcgtcct tgaggacgta gccctgcgct
     6961 ccgagagcca gcccctcctc gagcagggcc gggtcgtcgt acatcgtgag tagaataacc
     7021 ttcaaggtcg gcttctgctc tcgggtcatg cgcaccgcgg ccaatccgtc gagtttgggc
     7081 atgctgatgt cgaaaatggc gacgtcgatc ggatgcgtcc ggcagtactc gacggcctgc
     7141 tcaccgtccg aggcctcgaa gaccagccat ttgccccgct gtccctcaat agcctcgcgc
     7201 agtccgctcc ggatgatcgg gtggtcatca gcaatgagga ctcgtaccgt ggccatacgc
     7261 tatgaggaca tcgtacccga acccggctcc gagacaagcc gcaaaggggc tccgcggctg
     7321 ctgtcctcgc gcgcggtccc gatcgggagt tggagctcga cccgcgtccc tccgcccgga
     7381 tggctggcga tccaggccga cccgcccagc aacgcgcttc gttcgacaat ccccctgagg
     7441 cccagacctg acgaagccga gtcaccatgc cggatgcgag tgagatcgaa gccctggccg
     7501 gtatcctcga cagatgcgca gacccagcgc tgttgcgggg ccacgcacag gcgcgcctcg
     7561 gtggcctggg agtgcctcac cacgttggtg agacactcct ggaggatgcg gtaaagaccg
     7621 atttcggcct cgggcttgag aacgccgtcg atgttctgaa tctcggaaac gatgtggaca
     7681 gacgagagtt tggcgattga ctcgaccatg gcctggaggg cgcgggtcag tcccagtcgc
     7741 tcgacgagat aagggtggag atcctggacg atggcgcgga tttcgcggac ggcctgggcg
     7801 gcgagcgacg tgatttcctg gaggctttcg gcggtccgcc cggggctgac gccgttggcc
     7861 agacagcgct cggcatggtg tttgatggcc aggatattct gccccagtcc gtcgtgcaga
     7921 tccgtcgcga tgcggcagcg ctctctctcc tgggtctcga tcaatcggcg ggcgaacgat
     7981 tcgcgctccg ctttctcccg cttcaacgcc tcctcctgct cgagcttccg gatgcgcgcc
     8041 cggtcccgct gctgatcggt caggtcgtga atcaccacca tgccgacatg gcggccctgg
     8101 tatgggaatc gacgcgactg gatcgcgatc gggaaccgct ggccgtcccg cgtcagggcc
     8161 aggcattcgt aacgctgccc ggcgctggtg accagactct ggcgcacgag agtccgggat
     8221 tcgggctcga tgagatcgat cagagacttc ccgaggagtt ccgctggaga ccatccgaac
     8281 atgttctcca cgacgttgtt cgcctcctgg atgatgccgc agtcatgggt gaggatgcct
     8341 tcgagcaccg tttcggcctg gacccgcgcc agggtttccg catctcgcag tgcgtcggtc
     8401 gtccgcttca gctcggtgat atcgtgcgcc tccgcaatca acaagggact gatcccggcg
     8461 gggctgtcca ggggcctcag gaagaagtca atcgtcttga gctggttctc cgccccctgc
     8521 accacctcct cgtaccgcac tgtttcacct cgagcggcgc gctcgacagc ccgttgaacc
     8581 ttgtcttgcg tctccggcga cagtgtccac cagggggtca tccagaaggg catcccgcgc
     8641 acatcctcca tcttgagccc ggcgaatcgc agcgcggtgt cgttgatcat gatgacatgg
     8701 ccctccgcgc tgagcagacc catatactgg aaggaactgt tgaacacggc ccacagaagc
     8761 cgctcattcg caagcacctg ctcttcggtg cgcacgcgct ccgtaacgtc ccgagccacg
     8821 accaggatcg acgccagcac gccgcccgcc ccgaactccg gcacgaaccg ggtgtcatag
     8881 tagcgcctcc tctcgccaac cccgtgcgcg atctggccac cggcttccgc tccgctcgcc
     8941 accacccggt cgaagatcgc ccgcgccgat tcccatgttt cccgccccgt gcccaaatcc
     9001 cgcatccgcc tcccgcgcag gccatcaaga ttcttgccgt agagcttcgc cgcggccaga
     9061 tttgcataga tgtgccggcc cttgagatcg aagcggaaga tcaagtcttg cgagttctcg
     9121 accagtgtac gcacctcgtt ctcgcgaaaa cgcaatcgct cgatcgccgc ttcgagtgcg
     9181 cgcgtccgct cccggacctc atgctccaat tggtcgtgcg ctcgattcac acaagcctcc
     9241 ctgcccttcc gctgagtcag ctccatcgaa ggcccgggtg cccgctcgat cggcgcccgc
     9301 tggatgttcc catcgcgttt ggattcaggc cttgggaagt tggtcttctt catttggaat
     9361 cgaccgacat tctagcgcgc ctcccgcgaa tcgccagcgc aatcgcaagc ccctgctcgc
     9421 ccggttattt cacatgcttc gcgaggcgtc ggccgccggt ggccatggcc aaaacccagc
     9481 gcacacgaag tggcgacatg ctccctcggt ctgaggcgga gcctcgctaa ccccagagag
     9541 tgtaaatgaa gatttgcgcg acagcgacca ggatggccca ggcgcggaca ttccgccaga
     9601 gcgctgggcg gcattcagtc tcgccgagca tcctggccgg caggctggcg agcaggacga
     9661 tcaaggcgac aaacacgcca atgaacgccg ccttgcccat ccaacccgcc ccgcttgcgg
     9721 ccgcaagcag ccagagtgtg ccgagaggct ccgagaacat agtcatggct tgttccctcc
     9781 ctccgccggc agccagcaca ccaaaccgcg cagtcgtgcc tcgtcatgac cggaggtgaa
     9841 cgcgctcacg acgacgaccc cggccagcgc cgccacaaac gaccaccacg ccgtccacag
     9901 aaagggaatc ccaccgggca ctcccagcag aagctcggcc gaattcaaca cggccgaggt
     9961 ggacacgccg atgatcatgc cggccacacc gccccacggt gtggcgtgcc gggtcaacat
    10021 gcccagcaac aagagagcca gaagcggccc ttggaagaac gagagaaacg tctggaaggc
    10081 ctcgaacacg gatccgaagc ctttcttgat cgggtagctg atggccacgc ctcccaccag
    10141 gaaggcgacg accagccacc ggcccacgag aaggcattcg cgatccgcgg cctgtcggcg
    10201 gatgaatgtg cggtacaggt ccgtgaccac catcgtgctg gccgagctga cataggaatc
    10261 gagattcgag atgaccccgg cgaagaaggc gcccatcacc acgcccaaca cgcctggcgg
    10321 cagaagacgc accaccagca tcggcaagac gcggttcccg tcccagtctt tgcccggttg
    10381 gcccagttcc gggcccagca gcgccacgcc gaccaggccc ggcaccacca ggagaatcgg
    10441 aaac
//
LOCUS       DAOQRO010000083        10382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5708_length_10382_cov_5.693328, whole genome shotgun sequence.
ACCESSION   DAOQRO010000083 DAOQRO010000000
VERSION     DAOQRO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_10382_cov_5.693328"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3223)
                     /locus_tag="PKK20_04670"
     CDS             complement(<1..3223)
                     /locus_tag="PKK20_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99212.1"
                     /translation="MAEPLTMAAFLAATGVHIYYHVVGGAAYDLVGNFIEHWREAAMD
                     PQTGLPRNHDLREASEASFQAAVQLLLLELAGRIDPKRSWLTRWAEKGRWASVLAKDL
                     FAGTDQPHRQWLDAFRTAVGGRHFKSLHNSLNLSEDQMRECFKEANLCMVLGTAFADR
                     LLEWTRFHVTAGTEHPDFENLVRNGWRVDERAEANPPPLSAGLATKGVAVHHLGADRR
                     ITLAHAYCLFFREHVKRRPEVFRIFTADTLNEMLARLDRLGGEVKGELTTLRAGIAAV
                     ATNLPNSAQFGQWLTPQLGAIQDLLEEAKAQLDDVARSQLEMAGQQAEILVAITTVQT
                     ELRRGHPDARAAHEALFEHIKRLTMAFQRVERRLFGLPLVRPSVPHEPEPGSSDLWII
                     EAKYRSIDLVGREAEMDALWQWVHGEKPVSVRLITGDAGAGKTRLAFELIWRLAIEEG
                     DAWQAGRLISDDLRQLTQDRFSAKWEWDRPTLVMLDYARASHDALARLLTELARRAHD
                     PGLHPFRLILLERTPIGTQNWLETLFPLKSTDGGPRVRELFDPPAPVPLPAVTSADLR
                     RGLFAATLQRVARFQGRAPLPVPEAGADVVFDGLIAQPEWRDPLTLMMAALVAHQRQD
                     LLAGRNLKRADLAFELADAEGARLVKFAGRRLPPGEECLLRHVAGLTVLAGGLGREEV
                     QGLADTEADLLRYGWDQYHRRLGDVLATAWPGAEGSVAALQPDIVAEAFVLRNLAQRL
                     NRKDGIQAVRRLVKTHERTCAGLILRAFHNFQSNSACAETLAAWAQALVDDGLANRDF
                     ALLAALDDSMPRATTALRHVAARVTGARLRHLKDNPPADPDPAWHAELARLAGNLGVR
                     LSEAGRRSEALAPAQEAVELYRALARQNPDAFQPDLASALNTLANQLSELGRRSEALV
                     PAQEAVELYRALARQNPDAFQPALAMSLNNLANRLSELGRRSEALAPAQEAVELRRAL
                     ARQNPDAFQPALAMSLNTLATRLSELGRRSEALAPAQEAVELRRALAEENPDAFQPDL
                     AMSLNNLANQLSDLGRRSEALAPAQEAVELYRALARQNPDAFQP"
     gene            3359..3553
                     /locus_tag="PKK20_04675"
     CDS             3359..3553
                     /locus_tag="PKK20_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99213.1"
                     /translation="MFKGAGVSVEGGCFEAKLSSGFAPARNSRANYTEDLLCLRSFES
                     KARRCSERAGYGWFFLIFGD"
     gene            complement(3557..4951)
                     /locus_tag="PKK20_04680"
     CDS             complement(3557..4951)
                     /locus_tag="PKK20_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99214.1"
                     /translation="MPEKTINEVTRAWREQYEKGLAAFERNNLDYAIDILSKVVEQEP
                     AFFDCRQALRAAQFKKSGVGGGGLFKKIMGSTNPKVVQAQIALRNNPRHALNLVEDVL
                     NHDPNNMAAHRVLADAAMALDFPKTAVLSLEIVYKQSAKDRDVVMKLARALVKTGQAR
                     RAEGLIAELSRAHPRDPEIAQALKDMSASRTMTEGGYEGLADGKGSYRDILRNEEEAV
                     TLEQEKREHKTEDVTRRLLAEYEARLAAEPDNLRLVRSIAELYAEKKDYDKALEYYKR
                     LTGSEAGDPVVDRTIADLTVKRFDQALLDLDPTAPDHAEQSAKLNAEKQAFMLDACKK
                     RVDRYPNDLQLRFELAQLYFEAGRISEAIQEFQKSQNNPHKRIQSLFYLGQCFSRRGM
                     NDLAARTFQNAIKEKAVFDDEKKELIYALGCALEKMGKRNEAIEQFKLIYESDIAYRD
                     VAAKVDAYYASQGS"
     gene            complement(4972..6012)
                     /locus_tag="PKK20_04685"
     CDS             complement(4972..6012)
                     /locus_tag="PKK20_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNU99215.1"
                     /translation="MPKDITYKFCFGPWNLSEGQDPYGPPTRPVQTFDWKLGQLKRLG
                     FDAMMFHDDDAVPDVDGKSDAQLARESKELRKKLKDAGVVAEIVAPRLWFDPRTIDGG
                     YTSNDPKCRQFAIRRSLRCIDIANHLGCNLIVLWLAREGSYIRESKNARRSMDYLVAA
                     IDKMLAHDKRIRIAIEPKPNEPMDVAYVPTIGHALALASLTRDPKRVGCLIESAHALL
                     AGLDPADEIDFACSFGKLWSLHLNDQNGLKFDQDKPFGSANLRVAFNQVLALERNRYG
                     ARGEYVAFDVHPFRTTKVEHWTAHLENSRRTFLKLVEKARTYPEAQANKLIADRNYAA
                     LDQLVLEHLMGR"
     gene            complement(6080..6577)
                     /locus_tag="PKK20_04690"
     CDS             complement(6080..6577)
                     /locus_tag="PKK20_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99216.1"
                     /translation="MFDAMQSHELLREVYDRANVKQIAADLGLSLSLIYKWAEPSNDM
                     GSGTTNPLDRVVALIRSTGDSQIVQWICQEAGGFFIRNPKGLWPHPLLLVPATNLIVQ
                     DFADLLAVIAKAANDNHISRPEAAAIRTRWEELKSATEGFVRCCEAGNFTSMKPAESP
                     PADKR"
     gene            complement(6855..7868)
                     /locus_tag="PKK20_04695"
     CDS             complement(6855..7868)
                     /locus_tag="PKK20_04695"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HNU99217.1"
                     /translation="MFRGWHDFRSWDRTTKALSAEEVWTSPVVRVPNSWDELIASWNV
                     QTPSGSGLHIEARAGHADAWTGYYSLGFWSADPKQHRRASVSGQSDADAEVQTDTLVL
                     KRPAETAQLRVTLTASPQGARPVLTFVGLCCLNTRTKPQPLPPNTRAWGRSIDVPERS
                     QASYPGGAQAWCSPTSVSMVLAYWSRQLGKPELDRDVPEVVEGVYDPIWPGTGNWSFN
                     TAYAGSLPGLRAYVTRLTDLSELEDWVMRKVPVVVSVCYDLLRGRPRNRDSGHLVVCV
                     GFTKEGDAIVNDPGTLTGVSKTFPRENLARAWANSKNTVYLIHPEGWRLPHDRFGHWH
                     ATR"
     gene            complement(7969..8913)
                     /locus_tag="PKK20_04700"
     CDS             complement(7969..8913)
                     /locus_tag="PKK20_04700"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNU99218.1"
                     /translation="MNAAESELPPSPPLACIRAHSERQAMDWSLVLASQGIEAVIERH
                     PDTGVWRLWVAPEEHERAVGSIRLYRLENRGWAWQRRLVWPGVLFHWGALLWCWILIL
                     IHWLDQAVGYRFEAIGAMSSQAAARGEWWRLTTAVSLHADVGHLAANAATGLVVFGLA
                     MARFGAGWAMLIGLLAGTAGNAAGWVFHAKPFIGLGASGMVMGGLGMLTGQTLFLLWE
                     SPKAIRQIISGLLGGFLLFVLVGLNPASDVIAHAGGFVTGLLLGAAIALVPPRSNRRA
                     TANRTAGALALVLFCVTWTLAIVLGQNALLDHDLPRSY"
     gene            complement(8953..9585)
                     /locus_tag="PKK20_04705"
     CDS             complement(8953..9585)
                     /locus_tag="PKK20_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNU99219.1"
                     /translation="MTDSKPKAVIFDLGKVLLDFDYGIAIRRLAPKVQIPTDHIHTLV
                     NQSGLLHDYESGAIDTDQFFARVQAASGYRGTLDEFAAAFADIFVAIEPMIALHAQLR
                     RLGIPTYILSNTNELAIRHVICHYPFFSQFDGYVLSYEHRVMKPAPEIYAIAEALAGH
                     RGPDLLYLDDWPENVEAARCRGWRAVLHRSPEQSRAAVAAAGLLDADAAL"
     gene            complement(9578..10192)
                     /gene="recR"
                     /locus_tag="PKK20_04710"
     CDS             complement(9578..10192)
                     /gene="recR"
                     /locus_tag="PKK20_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNU99220.1"
                     /translation="MVDLVAALGRLPGIGPRSAERLAIHLVQTDSRQVCDLAQTLVAA
                     SERVRSCERCGGFTEHQPCRVCADPARDGSLLCLVEKPVDILLFEKAGSYRGLYHVLG
                     GKLSPLQGIEPEDLSIPALEKRLGIEPIREVILALPTDVEGDATSSYLARRLAGRSVR
                     ISRLAHGLPVGSALEYADELTLSRALEGRRAVPGTESDPAQHHD"
     gene            complement(10251..>10382)
                     /locus_tag="PKK20_04715"
     CDS             complement(10251..>10382)
                     /locus_tag="PKK20_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNU99221.1"
                     /translation="PADATLLEDLVLTAANSALARAREIANAEMAKITSGLSMPGLM"
BASE COUNT         1792 a         3196 c         3501 g         1893 t
ORIGIN      
        1 acggctggaa ggcgtcggga ttctgccggg ccagcgcgcg atacaattcc acggcctcct
       61 gcgccggggc caacgcctcc gaccgccggc ccaaatcgct caactggttc gccaggttgt
      121 tcagcgacat cgccaaatcc ggctggaagg cgtcgggatt ttcctcggct aacgcgcgtc
      181 gtaattccac ggcctcctgc gccggggcca acgcctccga ccgccggccc aattcgctca
      241 accgggtcgc cagggtgttc agcgacatcg ccaaagccgg ctggaaggcg tcgggattct
      301 gccgggccaa cgcgcgtcgt aattccacgg cttcctgcgc cggggccaac gcctccgacc
      361 gccggcccaa ttcgctcaac cggttcgcca ggttgttcag cgacatcgcc aaagccggct
      421 ggaaggcgtc gggattctgc cgggccagcg cgcgatacaa ttccacggct tcctgcgccg
      481 ggaccaacgc ctccgaccgc cggcccaatt cgctcaactg gttcgccagg gtgttcagcg
      541 cgctcgccaa atccggctgg aaggcgtcgg gattctgccg ggccagcgcg cgatacaatt
      601 ccaccgcttc ctgcgccggg gccaacgcct ccgaccgccg gccggcctct gacagccgca
      661 caccgaggtt gccggccaac cgggcaagct ccgcgtgcca ggccggatca ggatcggctg
      721 gaggattgtc cttgagatgc cgaaggcggg ctccagtcac ccgggccgca acgtggcgca
      781 gcgccgtggt ggccctcggc atcgagtcgt ccagggccgc cagcagcgcg aagtcacggt
      841 tggccaaccc atcatcgacc agagcctggg cccacgcagc cagggtttcg gcacaggctg
      901 aattcgactg aaaattgtga aacgcacgga ggatcaagcc ggcacacgtg cgctcgtgcg
      961 ttttcaccag gcgtcgcacc gcctgaatgc cgtccttgcg gttgaggcgc tgggccaggt
     1021 ttctcagcac aaacgcctcg gccacaatat caggttgcag ggcggccact gatccctccg
     1081 ctccgggcca cgcggtggcc agcacatccc ccagccgccg atggtattga tcccagccgt
     1141 agcgcagcag gtcggcctcg gtatctgcca acccttggac ctcttcgcgt cccagcccgc
     1201 cagccagcac cgtcagtccc gccacgtggc gtagcagaca ttcctcgcct ggcggtaacc
     1261 ggcgcccggc aaactttacc aggcgcgccc cttcggcatc ggccagttcg aaagcgagat
     1321 cggcgcgctt caaattccgt cccgccagca aatcctggcg ctgatgcgcg accagcgccg
     1381 ccatcatcaa ggtcagcgga tcgcgccatt ccggctgagc gatcagacca tcgaaaacga
     1441 catcggcccc cgcctccggc accggcagcg gcgcacgccc ctggaaacgg gccacccgtt
     1501 ggagtgtggc agcgaacaag ccgcggcgca ggtcagccga ggtcaccgca ggcagcggca
     1561 cgggcgcagg aggatcgaac aactcgcgca cccgcggccc gccatcggtc gacttgagcg
     1621 ggaagagcgt ctccaaccag ttctgggtac caatcggggt ccgctcaagc aaaatcaggc
     1681 ggaacggatg caggccagga tcgtgcgcgc ggcgggcgag ttccgtgagc agccgggcca
     1741 gcgcgtcgtg cgaggcccgc gcgtaatcga gcatgaccag cgtgggccgg tcccattccc
     1801 acttagcgga aaagcgatcc tgcgtgagct gccgcaaatc gtcgctgatg aggcgaccgg
     1861 cctgccaggc atcgccctcc tcgattgcca gccgccagat gagctcgaat gcgaggcggg
     1921 tcttgcctgc gccagcgtcg cccgtgatga ggcggaccga gaccggtttc tcgccgtgca
     1981 cccactgcca gagcgcatcc atctcggcct cccggccgac gagatcgatc gagcggtact
     2041 tggcctcgat gatccacagg tcgctgctgc cgggctcggg ctcgtgcggg acgctggggc
     2101 gcacgagggg caggccaaac agcctccgct cgacccgttg aaaggccatc gtgagccgct
     2161 tgatgtgctc gaagagggcc tcgtgtgcgg cgcgggcatc gggatgaccg cgcctcagct
     2221 cggtctgcac ggtcgtgatc gcgacaagga tttcggcctg ctggccggcc atttcgagct
     2281 ggctacgggc gacgtcgtcg agctgcgcct tcgcctcttc cagcaggtct tgaatcgcac
     2341 cgagttgggg agtgagccat tgcccaaact gagcggagtt cggcagattg gtcgccacgg
     2401 cggcaatgcc cgcacggagg gtcgtgagtt cacctttcac ctccccgccc agtctgtcga
     2461 gtcgggcaag catctcgttg agcgtgtcgg cggtgaagat gcgaaaaacc tctggtctgc
     2521 gtttgacgtg ctcgcggaaa aaaaggcagt aggcgtgggc gagggtgatg cgacggtcgg
     2581 cgcccaagtg gtgcactgca acccccttgg tggccagacc tgcggagagg ggagggggat
     2641 tggcctccgc tcgttcgtca acccgccaac cgttgcggac cagattctcg aaatcgggat
     2701 gttccgtgcc tgcggtgacg tggaaccggg tccactccag cagcctgtcg gcaaatgccg
     2761 tgcccaggac catgcagagg ttggcttcct tgaaacactc gcgcatctgg tcctcgctca
     2821 gattgaggct gttgtgaagg cttttgaagt gcctgccacc gacggcggta cggaaagcgt
     2881 ccagccattg acggtgcggc tggtcagtgc ccgcaaacag gtccttcgcc aggacactgg
     2941 cccaacgccc cttctcagcc catcgggtta accacgagcg cttggggtca atgcgcccgg
     3001 ccagttcaag gagcagcagt tgcaccgcgg cctggaacga tgcctcgctg gcctcccgaa
     3061 ggtcgtgatt tcgcgggaga cccgtctgcg ggtccatggc cgcctcgcgc cagtgctcga
     3121 tgaaattccc gacaagatcg tacgccgcgc caccgaccac gtggtagtag atgtgaaccc
     3181 cagttgcggc gaggaacgcg gccatggtca gcggctcagc catgcgggct ggaggctagc
     3241 cccggaggtg gcaagctgac aagccgtttc tcggcctttt cctccgggct ggactttgag
     3301 ctgatggccg atgagccact ggtgggccaa acaatcgtct ggcttcatcc atcagcggat
     3361 gttcaaaggc gctggcgtaa gtgtcgaggg tggctgcttt gaggcgaagt tgtcgtccgg
     3421 ttttgcgcca gccagaaaca gccgtgcaaa ctacaccgag gatttgcttt gcctcagaag
     3481 ttttgagtcc aaagcgcggc gctgcagcga gcgcgccggc tatggttggt tcttcctgat
     3541 cttcggtgac tgacccctac gagccctgtg aggcgtagta ggcgtcgact ttggcggcga
     3601 catcgcggta ggcgatgtcg ctttcgtaga taagcttgaa ctgctcgatg gcctcgttgc
     3661 gtttgcccat cttctcgaga gcgcagccga gggcgtagat cagttccttc ttctcgtcgt
     3721 cgaacaccgc cttctccttg atcgcattct ggaaggtgcg cgcggccaga tcgttcatgc
     3781 cgcggcggga gaagcactgg ccgagataga agagagactg gatgcgtttg tgggggttgt
     3841 tctgggattt ctgaaactcc tggatggcct cgctgatgcg tccggcttcg aagtagagct
     3901 gggccagttc gaagcgcagt tggaggtcgt tgggatagcg gtccaccctc tttttgcagg
     3961 catccagcat gaaggcctgc ttctcggcgt tgagtttggc tgactgctcg gcatggtccg
     4021 gcgcggtggg atcaaggtcg aggagcgctt gatcgaagcg cttgaccgtg aggtccgcga
     4081 ttgtgcggtc gacgaccgga tcccccgcct cgctgccggt caggcgcttg tagtattcga
     4141 gcgccttgtc gtaatccttc ttttcggcgt agagctcggc gatcgagcgc acgagcctga
     4201 gattgtccgg ttcggcggcc aggcgggcct cgtattcggc cagaagccgg cgcgtgacgt
     4261 cttcggtctt gtgctcccgc ttctcctgtt cgagcgtcac ggcctcctcc tcgttgcgga
     4321 ggatgtcgcg gtaggaaccc ttgccgtcgg ccaagccctc gtaaccgcct tcggtcatgg
     4381 tgcggctggc cgacatgtct ttgagggcct gggcgatctc ggggtcgcgc gggtgggcgc
     4441 ggctcaactc ggcgatgagg ccctcggcgc gccgggcctg gccggttttg acgagtgcgc
     4501 gcgccagttt catgacgaca tcgcggtcct tggcggactg tttgtagacg atttcgaggg
     4561 agagaacggc cgtcttaggg aagtcgagcg ccatggccgc atccgccagc actcgatggg
     4621 cggccatgtt gttcgggtcg tgattgagga cgtcctcgac gaggttcaat gcgtggcgcg
     4681 ggttgttgcg cagggcgatt tgggcctgga ccaccttggg attggtcgag cccatgatct
     4741 tcttgaacag cccgccgccg cccacgccgc tcttcttgaa ctgggcggcg cgcaaggcct
     4801 ggcggcagtc gaagaaggcc ggctcctgtt cgacgacctt gctgagaata tcgatggcgt
     4861 aatcgaggtt attccgctcg aaggccgcca ggcctttctc gtattgctcg cgccatgcgc
     4921 gtgtgacttc gttgatggtc ttttcgggca tggtctcccc cccgcctcgt gctaacggcc
     4981 catcagatgt tcaaggacaa gttgatcgag cgccgcgtag ttgcggtcgg cgatgagttt
     5041 gttcgcttgt gcctccgggt aggtgcgggc cttctcgacc agcttgagga aggtgcgccg
     5101 gctgttctcg aggtgggcgg tccaatgctc gaccttggtg gtgcggaagg ggtggacatc
     5161 gaaggcgacg tactcgccgc gggcgccgta ccggttccgt tccagggcaa ggacctgatt
     5221 gaaggcgacg cggaggttgg cgctgccaaa gggtttgtcc tggtcgaatt tgagtccgtt
     5281 ttggtcgttg agatgcagcg accagagctt gccgaaggag caggcgaaat cgatctcgtc
     5341 cgccggatcg agtcccgcga gcagggcgtg cgcggattcg atgaggcagc ccacgcgctt
     5401 ggggtcgcgg gtgaggctcg ccaaggccag ggcgtggccg atggtcggca cgtaggcgac
     5461 gtccatgggc tcgttgggtt tgggctcgat ggcgattcgg attcgcttgt cgtgggcgag
     5521 catcttgtcg atggccgcga ccagatagtc catcgagcgg cgggcgtttt tgctctcgcg
     5581 gatgtagctg ccttcgcggg ccagccacag gacgatgagg ttacatccga ggtggttggc
     5641 gatgtcgatg cagcgcagcg agcggcggat agcgaattgg cgacacttgg gatcgttgct
     5701 tgtgtacccg ccgtcgattg tcctcggatc gaaccacagg cgcggcgcga cgatttcggc
     5761 gaccacgccg gcgtctttga gtttcttgcg caactccttc gattccctcg ccaattgcgc
     5821 gtcggatttg ccgtcgacgt ccgggacggc gtcgtcgtca tggaacatca tggcgtcgaa
     5881 gccgagccgt ttcagttggc cgagcttcca atcgaacgtc tggacggggc gggttggggg
     5941 gccgtagggg tcctgacctt cgctcaaatt ccagggacca aagcagaact tgtaggtgat
     6001 gtctttcggc atatgtgaat cgaggttaga agaacagagg gatgactgaa aagaaaaaag
     6061 gcggcatgcg cttctcgggt cagcgcttgt ccgcgggcgg ggattcggca ggcttcatcg
     6121 aggtgaaatt gcccgcctca cagcaccgca cgaagccttc ggtggcggac ttgagttcct
     6181 cccagcgggt gcggatggct gcggcttcgg ggcgggagat gtggttgtcg ttggcagcct
     6241 tggcgatcac cgccaggagg tcggcgaagt cctggacgat caggttggtg gcggggacaa
     6301 ggagcaaggg gtgcggccag aggcccttgg gattgcgaat gaagaagcct ccggcttcct
     6361 ggcagatcca ctgcacgatt tgggaatcgc ccgtgctgcg aatcaaggcc acaacgcgat
     6421 cgagcggatt ggtcgtgccg ctgcccatgt cgttcgacgg ttcggcccac ttgtaaatga
     6481 gcgacaaaga cagtccgaga tcggcggcga tctgtttgac gttggcccgg tcgtaaacct
     6541 cgcgaagcaa ctcgtgcgac tgcatggcat caaacatggg caacccgctg ggcattgaca
     6601 agcgggaagg cgggcgggaa ggcgggccgt gtatctggag gttgtcggcg ggcgtcgagt
     6661 ttgcgccgcg tggggcctgc ctgccgcgcc tgcctgtgcc gtgcccgaca cggcagacag
     6721 gccgcagcgc tttgcggcgc aggcagggca cgcggcctgc accgggagtc tcgatccagt
     6781 gtcggcccgg tgcacttacc gggcggcatc gcgccgacaa ccagcagatg caccgaaggc
     6841 gggcgaacgg ctgactacct cgtcgcgtgc cagtgtccga agcggtcgtg cggcaggcgc
     6901 cagccctcgg gatggatcaa gtagaccgtg ttcttcgagt tggcccaggc gcgtgcgagg
     6961 ttctcgcgcg gaaacgtctt gctgacgcct gtgagcgttc ccggatcgtt cacgatcgcg
     7021 tccccctcct tggtgaaacc gacgcagacg accaggtgtc cgctgtcgcg gttgcgcgga
     7081 cggcctcgca agaggtcgta gcagaccgag acgacgacgg gcactttgcg catcacccag
     7141 tcttcgagtt cggacagatc ggtgaggcga gtcacatagg cccgcaggcc gggcagcgag
     7201 ccggcgtagg ccgtattgaa cgaccagttg cctgtgccgg gccagattgg gtcgtataca
     7261 ccttccacca cctccggcac gtcacggtcc agttccggtt tgcccaattg ccgcgaccaa
     7321 taagcgagca ccatcgagac gcttgtgggg ctgcaccagg cctgggcgcc gccggggtag
     7381 cttgcctggg agcgttcggg gacatcgatc gaccggcccc acgcccgggt attgggcggg
     7441 agtggctgtg gcttcgtccg cgtgttcagg cagcagagcc cgacgaaagt cagcacgggg
     7501 cgcgcgcctt gcggcgatgc cgtcaaggtc acccgcagtt gggcggtttc cgcgggccgt
     7561 ttgagcacga gggtgtcggt ttggacttcg gcatcggcat ccgactgtcc ggaaacgctg
     7621 gcgcgccggt gctgcttggg atcagccgac cagaatccga ggctgtagta accggtccat
     7681 gcatcggcgt gtccggctct ggcctcgatg tgcagtccgc tgcccgaggg cgtctggacg
     7741 ttccaggacg cgatgagttc atcccaggaa ttgggcaccc gcacaaccgg tgaggtccac
     7801 acttcttcgg cggagagggc tttggtcgtg cggtcccaac ttcgaaagtc gtgccagccg
     7861 cgaaacatgc ttgcgtgatg gtcggttgtg cgcggttgtg cgatcgcagg ccgggcaagg
     7921 acgccgcaca ggctcaagac ggttgaggcg caaaccaagc gccatgtctt agtagctacg
     7981 tggcagatca tggtcaagca gggcgttttg gccgagaacg atcgccagcg tccaggtgac
     8041 gcagaagagc accagggcca gggctccagc ggttcggttg gcggtggcgc ggcggttcga
     8101 gcgcggcggc acgagtgcaa tggcagctcc gagcagaaga ccggtgacga acccgccggc
     8161 atgggcgatc acgtcgctgg cggggttgag tcccaccagg acaaagagca gaaagccgcc
     8221 gagcagtccc gagatgatct ggcggatggc cttggggctt tcccagagca ggaacagcgt
     8281 ctggcccgtc agcatgccca ggccacccat caccattccc gaagcgccca gcccgatgaa
     8341 aggcttggca tggaagaccc aacccgcggc gttgcctgcc gtgcccgcga gcaggccgat
     8401 gagcatggcc cagcctgcgc cgaaacgggc catggcgagc ccgaacacga ccaatccggt
     8461 tgccgcgttg gcggccagat gtccgacgtc ggcgtgcaga ctgaccgcgg tggtcaatcg
     8521 ccaccactcg ccacgggcgg cggcctggct gctcatggct ccgatggcct cgaagcggta
     8581 gccgaccgcc tgatcgagcc aatggatcag aatcagaatc cagcaccaca gaagggcgcc
     8641 ccaatgaaac agaacgccgg gccataccag ccggcgctgc caggcccatc cacggttctc
     8701 gagtcgatac aaccggatcg atcccaccgc gcgctcgtgt tcttcgggcg ccacccagag
     8761 cctccacacg ccggtgtcag gatggcgttc gatgacagct tcgatgccct ggctggccag
     8821 gaccaggctc cagtccatcg cctgtctttc cgaatgggct cggatgcagg ccagaggcgg
     8881 gctcgggggg agctccgatt cggcagcatt catgggggcg tggcgatgcg gagctgttcg
     8941 cgcaaaacgt cgtcagagcg ccgcatccgc atcgagcaat ccggcggcag cgaccgcggc
     9001 ccgcgattgt tcgggtgacc gatgcagcac agcgcgccaa ccccggcatc gcgctgcctc
     9061 gacattctcg ggccagtcat cgaggtagag gaggtcgggg ccgcggtggc cggccagcgc
     9121 ctcggcaatg gcatagattt cgggcgccgg tttcatgacc cggtgttcgt aggagagcac
     9181 gtagccatcg aactggctga agaacgggta gtggcaaatg acatggcgga tggccagctc
     9241 attggtgttc gagagaatgt atgtgggaat cccgagtcga cgcaactggg cgtgcaaggc
     9301 gatcatcggc tcgatcgcaa cgaagatgtc cgcaaaggcc gctgcgaact catcgagtgt
     9361 accccgatag cctgaagccg cctggacacg cgcgaaaaac tggtcggtgt cgatggcccc
     9421 gctctcgtaa tcatggagca gtcccgactg attaacgagg gtgtggatat ggtcggtcgg
     9481 gatttggacc ttgggcgcca gccggcgaat tgcgatcccg tagtcaaagt ccaggaggac
     9541 cttgcccagg tcaaagatca ccgccttggg cttcgagtca gtcatggtgc tgtgcggggt
     9601 ccgattcagt gcccggcacg gcacggcggc cttccagagc ccggcttaag gtcagttcat
     9661 ccgcatactc gagggcgctc ccgaccggca ggccgtgggc aagccttgat attcgaacgg
     9721 agcgcccagc caggcgtcgg gccagatagg aactggtcgc gtcgccctcg acgtccgtgg
     9781 gcaacgcgag aatgacttcg cggattggct cgatcccgag ccgtttctcg agtgcgggga
     9841 tcgacaagtc ctccggctcg atgccttgga gcggggagag cttgccgccg agcacgtgat
     9901 agagtccgcg atagctgccc gccttctcga acaggaggat atcgaccggc ttctcgacca
     9961 ggcagagcaa cgagccgtct cgagcggggt cggcgcagac gcggcacggt tggtgttcgg
    10021 tgaacccgcc gcagcgttcg caggagcgca cgcgctcgct tgctgcgacg agggtctgag
    10081 ccaagtcgca cacctgacgc gaatcggtct gcaccaagtg gatggcgagg cgctcggcgc
    10141 tacgcgggcc gatccccggc agcctgccca gcgccgcaac caggtcaacc atggggccgg
    10201 gcaagtgcga catcctgaaa tcctcgatcg gatgcaaaca cgatcccgga ctacatcagc
    10261 cccggcatgc tcaagccgct ggtgatcttg gccatctcgg cgttcgcgat ctccctggcg
    10321 cgcgccaggg cgctgttcgc ggcggtcaaa accaaatcct cgaggagggt ggcatcggcc
    10381 gg
//
LOCUS       DAOQRO010000084        10284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5788_length_10284_cov_5.470916, whole genome shotgun sequence.
ACCESSION   DAOQRO010000084 DAOQRO010000000
VERSION     DAOQRO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5788_length_10284_cov_5.470916"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..636
                     /locus_tag="PKK20_04720"
     CDS             157..636
                     /locus_tag="PKK20_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNU99222.1"
                     /translation="MAIAVGSKAPDFRLKSKTAEEIVDVKLSDNFGRKNTVLLFFPLA
                     FTGVCTQEMCDISSGLNTYAQLGADVIAVSVDSPFAQSAWAEKEKIRVTLASDLNKKV
                     AADYGVLLADLGGLGSTAARAAFVIDKAGVVRYSEQTPTPKDLPNFDAIKACLATLQ"
     gene            725..1657
                     /locus_tag="PKK20_04725"
     CDS             725..1657
                     /locus_tag="PKK20_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNU99223.1"
                     /translation="MRHLDAIRLCCLLLGACWLSGCLPLPDQESEEKNPLILDARAKK
                     AAYNFQGAVASLEKALEANPRLALAHWELGLILCKDLSDPAGAIYHFNRLLKLRPEWR
                     HAETARQRIRDCQVEIAKSVPLGPQLPAFQQQFDRMVSRVHDMDKEINLLRVTNQTLH
                     MHVQQLWAENTQLREALRTASAAAAAPALPPTTTASGAPIAAAQPRPPSETQQSNRAV
                     PGPDPTLSASASTRPNAAVNPLSDARRPSVSVSSASAVPAPRGYLQYTVRKGETMSTI
                     ARRYGLPLRALLSANPNINPNRLNVGQVIRIPAM"
     gene            1669..2484
                     /locus_tag="PKK20_04730"
     CDS             1669..2484
                     /locus_tag="PKK20_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99224.1"
                     /translation="MVKRMYFPLVILFWVIMNVLLWRSEMSSHQNSGGRVPIATVWER
                     ILTAPDDSALQILHRGQKIGFCRWVATIDETAMARTEAEQETVEGRVRAFAGYTIDLD
                     GNLLLDESPKRLRFAMHAVFGVDQAWQKFRVRLNLKPNAWEIEADAEAERLVLRTGEP
                     GSEWEQAVAFADLARPEKLMAQFGLPPAIAGLAALTRDLRVPDAAVLARGLAWGARSD
                     WFALGHSRVRVYRIEARLLDTHRASVVVSRVGEILRVELPDDIVLVNEAIAAL"
     gene            2497..3276
                     /locus_tag="PKK20_04735"
     CDS             2497..3276
                     /locus_tag="PKK20_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99225.1"
                     /translation="MIELIHLVKRFGDLVAVNAVDLVVPRGQFFALLGPNAAGKTTTV
                     KILAGLMKPTSGAARIAGYDVQEQSLEARRRIAYVPDFPFLYDKLTPWEFIRFTGQLF
                     QMPEERIAAGARELVARFNLEPYLAKPIEALSHGTRQRIVIVSALLHAPEVVVLDEPM
                     VGLDPHHARVLKDILKERALAGMTVFVSTHQLSVAEEMADRIGIMHEGQLIAVGTRDE
                     LRRQSGTSGALEQTFLTLTRQEANLREEHPDSAGAPASHSQ"
     gene            3273..5036
                     /locus_tag="PKK20_04740"
     CDS             3273..5036
                     /locus_tag="PKK20_04740"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99226.1"
                     /translation="MNRPLSTNRAPAPRAASPSPLTLLVLTNMRQAWRRLLTIRQQSR
                     LLTALIVLFLGGYATLAFAIFYKGLRFAGSFPGLGAVLIERMLFLLFAFLFGLLLLSN
                     LIISYTNLFRNRETSFLLPLPIPWASIFRWKFIESALLASWAFLFLIAPMLAAYGLTQ
                     GVPWHFYAATLGFLGLFIMLPAVAGSWLAVNLARFLDRRAFQIAALFVASSLLAATAI
                     WLKTDPITDEQLETRVLVVLDRLLSRTHFALFPLLPSYWLSAGVQQWAEGALGAALFF
                     ALVLLSHVLFFGTLALTRMGNAFYAAVSMVQSRGSAMNRWPWFRAWQQRLDRHARSTG
                     WLERALDRARPIPVDIRAVLLKDMRLFWRDTTQWGQTLVLFGLLGVYFLNLRHFSQQL
                     TSPFWVFLVSYLNLGACALNLATLTTRFVYPQFSLEGKRLWIVGMAPLGLRRLLLAKF
                     ALTSTLSLALTAGLIILSCRMLMLAWDRTFYLTVAITVMTFTLNGLAVGLGALHPNFK
                     EDNPSKIVSGFGGTFCLVLSFLYIVASVVLLALGSPWTRSGEPGGFGLLPSWAAFVVL
                     SAAVGWLPLRLSLRRINQFEM"
     gene            5045..5542
                     /locus_tag="PKK20_04745"
     CDS             5045..5542
                     /locus_tag="PKK20_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99227.1"
                     /translation="MIRTPRLARVLVRWFATAARDLPWRRTRNPYAVWIAEVMLQQTT
                     VAAVVPHYTRWMRELPTLGALATASPERVYRLWEGLGYYRRADNLLRAAWIIAARPDE
                     LFPRRFAERLALPGLESDAAPWRQMLRRAIRRGFLKSSIALVFLAGGFFVVEALLRAP
                     RYCWR"
     gene            complement(5539..5763)
                     /locus_tag="PKK20_04750"
     CDS             complement(5539..5763)
                     /locus_tag="PKK20_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99228.1"
                     /translation="MTVDHLLLTYVVVLLTGVSAIAVYYELRRRSFGPTGTEDHIFRC
                     GRCGYVYTDDPDVDLSRCPQCGQMNEIFEF"
     gene            complement(5760..6533)
                     /locus_tag="PKK20_04755"
     CDS             complement(5760..6533)
                     /locus_tag="PKK20_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNU99229.1"
                     /translation="MNPHAPHGFKRWDRARWWGSVAGVFLLQVIIILVLSPRPPLSAT
                     PAQRLARLRLITQPISQAQLESRMWLSDPAEIALVSARGFSGPVWLQLPRMESGLRHW
                     TEPPRWLTQDVSRLATLPILPETEPVRFPLPAAPAATPFLGATSASSSSAPPQSTVRV
                     EGDLARRRRLDSPELPSWPYNDVLAATTVQLVVDRNGSVLAANLLTRSGLPAADQRAL
                     ELARAAQFEQANPASGASSLTWGQMMFQWQAVPPATNAP"
     gene            complement(6530..6979)
                     /locus_tag="PKK20_04760"
     CDS             complement(6530..6979)
                     /locus_tag="PKK20_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNU99230.1"
                     /translation="MRYQRNLKPFTGRLDVAPIAGVLFILVLFLVLQTSIAPGPGLRI
                     QLPSVALEDEPAPRGPVLVVTVDQHELVYFEHQVIGDEELKARLAAKSEQSRHPLSLL
                     VQADASVKHEAIVRLAALARQAGIRDIVIGTRPPLFPLEIDHPPGNP"
     gene            complement(7032..7649)
                     /locus_tag="PKK20_04765"
     CDS             complement(7032..7649)
                     /locus_tag="PKK20_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNU99231.1"
                     /translation="MLPDLLSHGGSMMWLLLVAGAVAVAVFIERLLHYHREQINTLDF
                     INGVRNVLKRDNVVEAISICDATPGPVARLVKTAILNRERGREGVREALEDAGLVEVP
                     RLEEKLALLATIGQIAPIMGLLGTVLGFIEVFRVLQTQGLSAPATLLAEGVWQALVCT
                     GAGLAVAIPCYAGYNYLVSRVNEIVVDMEKASSEIVSLLTDLQKL"
     gene            7808..>10284
                     /gene="pheT"
                     /locus_tag="PKK20_04770"
     CDS             7808..>10284
                     /gene="pheT"
                     /locus_tag="PKK20_04770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNU99232.1"
                     /translation="MNVTLNWLKTYVDFDWSADELAERLTMLGLEVEGVNRLAGEFEG
                     IVVAQVLTRDKHPNADKLSLCRVFDGQGERQIVCGAQNFQAGDKVPLILPGHSLPSKP
                     DEPPFTIKVGKIRGVESHGMLCSPKELGLAEDALGLMILRADAPVGQPFAAYLGRTPG
                     DVVYDLEVTPNRPDLNSVVGIAREIAALTGHPLRLPALTLPSAPSGAVGAADLVAVRI
                     EEPDLCPRYTARVLRGVKVGPSPDWMRALLERVGQRSINNVVDATNFVMLEIGQPLHA
                     FDYHLIARGPATSGESVSGSDAGGATPTIVVRRARAGEAFVTLDGQNHCLTEEMLLIA
                     DEEKGIALAGVMGGQNSEINEQTIDVLLESACFKPTNIRRTSKALGLRTDASYRFERG
                     VDAAMTDWASQRCAQLILEAAGGCLAAGVVDAYPRPVEPRQITLRHHKVNELLGIEIL
                     AADIEYYLGQLGLKVVGRKPRPVGDSDAAEPVTFAIPTFRPDLKREADLIEEVCRLHG
                     VDKIPAALPRGARGASEYDPIHDQLALARQLLSGLGLYEAQGQTLIADSAAALVEAAS
                     PGADARRPAGIVALANPLSSDMNVLRPSLLPGLLESLKHNLSRQNQDVALFEVGRVFL
                     HSDDGVGEHRRIGLALTGRRQPTFWSGTDRDAKFDVSDLKGLVEEFLEQYGLRGVSYA
                     RTLDPPPLFVESAAILLGGKLPLGVLGQLAPALAKRYDLRDPVFLAELDLAQLLVRRN
                     TAKGFKALPAYPAIRRDLAIVVPQSVTHEAVLAAVKQAKAQNLESVELFDVYQGKKIA
                     SGQKSLAYAFTYRSAARTLTDAEVNAA"
BASE COUNT         1734 a         3377 c         3282 g         1891 t
ORIGIN      
        1 aagtccagca aaatatgctt tccctgaaat cacccaacca atttgtcatc cacccgcccg
       61 cggtgatttg acagtggccg gaggcgccac tatacattgg ccctgtcgcg ttgtggcgct
      121 gcaacaaaac caaaacgaac agaaaaccgc aatcatatgg caatcgcagt cggctccaaa
      181 gcaccggact ttcgtctcaa atccaaaacc gctgaagaga tcgtggatgt gaagctgagc
      241 gacaacttcg ggcggaagaa caccgtcctt ctcttcttcc ccctggcctt caccggcgtc
      301 tgcacgcagg agatgtgcga tatcagctcc ggcctcaaca cgtatgcgca gctcggtgcc
      361 gatgtcatcg cggtgagtgt ggacagcccc ttcgcccaat ccgcctgggc cgagaaggag
      421 aagatcaggg tcaccctcgc cagcgatctc aacaagaaag tggctgcgga ctacggcgtc
      481 ctgctggccg atctgggtgg actcggctcg accgccgccc gcgccgcgtt cgtgatcgac
      541 aaggccggtg tggttcgtta cagcgagcaa acgccgactc ccaaggacct gccgaacttc
      601 gatgccatca aggcttgcct cgccactctg caataggcgc cgccgacgcg gccggctccg
      661 ggcgggttga tgcatttgtg tttcgcggat tgcaccgcgc gggttgtctg ttaaggttcg
      721 gtccgtgagg catctcgatg cgattcgtct ttgttgcttg ctgctgggcg cgtgttggct
      781 cagcggctgc ctcccgttgc cggatcagga gtcggaagag aaaaaccctt tgattctcga
      841 tgcccgcgcc aagaaagcgg cgtacaactt ccagggcgcg gtggcctcgc tcgaaaaggc
      901 cctcgaagcc aatccacgcc tggccctggc ccactgggag ctggggctga tcctctgtaa
      961 ggatttgtcc gacccggccg gggcgatcta ccacttcaac agactcctga agctgcggcc
     1021 ggaatggcgc cacgctgaga ccgcccggca gaggatcagg gactgtcagg tggagatcgc
     1081 caagagcgtg ccgctgggtc cccagttgcc cgcttttcaa caacagttcg accgcatggt
     1141 ctcgagggtc catgacatgg acaaggagat caacctgctc agggtgacca accagactct
     1201 gcacatgcac gtgcagcagc tctgggccga aaacacccaa ctcagagagg cgttgcgcac
     1261 cgcctccgcc gcggccgccg cccccgccct gcctccgaca accacggcct ccggagcgcc
     1321 aatcgccgcc gcccagccgc gtccgccgtc ggagacacag cagtccaacc gcgccgtgcc
     1381 cggcccggac ccgactctgt ctgcctcggc ctcgacccgt cccaatgccg cggtcaatcc
     1441 gttgtctgat gcgcgcaggc cttcggtgtc ggtctcatcc gcgtcagcgg tgccggcccc
     1501 acgcggctac ctccagtaca ccgtcagaaa aggggagacg atgagcacaa tcgctcgccg
     1561 ctatggcctt ccgctccgcg cgcttctctc cgccaatccg aacatcaatc cgaatcggct
     1621 gaacgtgggg caggtcatcc gcatcccagc aatgtaatcc gcgccgccat ggtcaaacgg
     1681 atgtactttc cgctggtcat cctgttttgg gtgatcatga atgtcctgct ctggcgctcg
     1741 gaaatgagca gccaccagaa cagcggcggc cgcgtcccca tcgccactgt ctgggagcgc
     1801 atcctgacgg cgcccgacga ctcggcactc cagattctcc atcgcggaca gaagattggg
     1861 ttctgccgtt gggtggcgac catcgatgaa accgcgatgg cgcgcacgga agccgagcaa
     1921 gagaccgtcg agggccgtgt tcgggcattc gcgggctaca cgatcgacct ggacggcaat
     1981 ctgctcctcg atgaatcgcc caaacgactg cgatttgcca tgcatgcggt ctttggcgtc
     2041 gaccaggcct ggcagaagtt ccgggttcgt ttgaatctca agcccaatgc gtgggagatc
     2101 gaggcggacg cggaggccga gcggctcgtg ctgcgcactg gcgagcccgg gagcgagtgg
     2161 gagcaggccg tcgccttcgc cgatcttgcc cgccccgaga aactcatggc gcaattcggc
     2221 ctgccgcccg ccatcgccgg gctggccgcc ctgacacggg atctgcgggt gccggatgcg
     2281 gctgttctcg cgcgcgggct ggcatggggg gcgcggagcg actggttcgc gctgggccac
     2341 tcgcgcgtgc gcgtctaccg catcgaggcc cggctgctcg acacgcaccg cgcatcggtc
     2401 gtggtgagcc gcgtgggcga gattctccgg gtcgagttgc cggacgacat cgtgctggtc
     2461 aacgaagcga tcgccgcctt gtagaatcct tgaccgatga tcgaactgat ccatctggtc
     2521 aaacggttcg gcgacctggt cgcggtgaac gcggtggacc tggtggtgcc ccggggccag
     2581 ttcttcgcgt tgctcggccc gaacgccgcc ggcaagacga cgacagtgaa gattctcgcc
     2641 ggcctcatga aaccgacctc cggagcggcg cgcatcgccg gctacgacgt tcaggaacag
     2701 tcgctcgagg cgcgtcgcag gatcgcgtat gtgccggact ttccgtttct ctacgacaag
     2761 ctgacgccct gggagttcat ccgtttcacg ggccagcttt ttcagatgcc cgaggagcgg
     2821 atcgccgcgg gagcgcgcga actcgtcgcg cgtttcaacc tcgaacccta cctcgccaag
     2881 ccgatcgagg ccctctcgca tggaacccgg cagcggattg tgatcgtctc ggccctgctg
     2941 cacgctccgg aagtggtcgt gctcgatgag ccgatggtgg gcctggatcc gcaccacgcc
     3001 cgggtgctca aagacattct gaaggagcgc gcgctcgccg gcatgacggt gttcgtctcg
     3061 acccatcaat tgagcgtggc cgaggaaatg gcggatcgga tcggcatcat gcatgagggc
     3121 cagctgatcg ccgtcggcac gcgcgacgaa ctgcggcgtc agagcggcac gtccggggcg
     3181 ctcgagcaga ccttcctcac gctcacgcgc caggaagcca atctgcgcga ggagcaccca
     3241 gactccgcgg gcgcgcctgc ttctcacagc caatgaacag gccgctgtcc acgaaccgcg
     3301 ccccggcgcc tcgcgccgcc tcgccgagcc cgttgacgtt gctggttctg accaacatgc
     3361 gccaggcctg gcggcgcctg ctcacgatcc gacagcaatc gcgtctgctg accgctctga
     3421 tcgtgttgtt tctcggcggc tacgcgacgc tggcattcgc gatcttctac aaagggttgc
     3481 ggttcgccgg ttcctttccg gggctgggcg cggtgctgat cgagcggatg ctcttcctgt
     3541 tgttcgcgtt cttgttcggc ctgctgttgt tgagcaacct gatcatcagc tacaccaacc
     3601 tgttccgcaa tcgggagacg tcgttcctgt tgcctctccc gattccctgg gcgtcgatct
     3661 tccgttggaa gttcatcgag tccgcgctcc tggcgtcgtg ggcgtttttg tttctgattg
     3721 ctcccatgct ggccgcctac gggctgactc agggcgtgcc gtggcatttc tacgcggcga
     3781 cgctcgggtt cctcgggctc ttcatcatgc tgcctgccgt ggctgggtcc tggctggcgg
     3841 tgaatctcgc ccgttttctt gatcgccgag cgttccagat cgccgccttg ttcgtggctt
     3901 caagcctcct ggccgcgacg gcaatctggc tcaaaacgga tcccatcacg gatgagcaac
     3961 tcgagacgcg ggtgctggtg gttctcgacc gcctgctgtc gcgaacccat ttcgcactct
     4021 ttcccttgct ccccagctac tggctctcgg ccggcgtcca gcaatgggct gaaggcgcac
     4081 tgggcgccgc gttgttcttc gcgctggtcc tcctcagtca cgtgctgttc ttcggcacac
     4141 tcgcgctcac gcgcatgggc aacgcgtttt acgccgccgt ctcgatggtc caaagccgcg
     4201 gcagcgcgat gaaccgctgg ccgtggtttc gggcatggca gcagcgactc gaccgccacg
     4261 cccgctcgac cggctggctc gaacgcgccc tagatcgagc gcgtccgatc cccgtcgaca
     4321 tccgcgcggt gctgttgaag gacatgcggt tgttctggcg ggacacgact caatggggcc
     4381 agaccctggt gctcttcggg ctgctgggcg tctacttcct caacctccgg catttcagcc
     4441 agcaattgac cagtcctttc tgggtcttcc tggtctcgta tctcaacctc ggcgcctgcg
     4501 cgttgaatct ggcgacgctc acaacccgct tcgtctatcc gcagttctcg ctcgaaggca
     4561 agcgcctctg gattgtcggg atggcgccgc tggggttgcg gcgtctgttg ctcgccaagt
     4621 tcgccctgac aagcactctg tcgctggcgc tcaccgccgg cctgatcatc ctttcctgtc
     4681 gcatgctcat gctggcgtgg gaccgcacgt tctacctcac ggtggcgatc acggtcatga
     4741 ccttcacgtt gaacgggctc gccgtgggcc tcggcgcgct ccaccccaac ttcaaggaag
     4801 acaacccaag caagatcgtc agcggcttcg gcggcacgtt ctgccttgtc ctcagtttcc
     4861 tctacattgt cgcctccgtc gttctcctgg ccctgggctc gccctggacc cgctccgggg
     4921 agccaggcgg cttcggcctc ctgccgagct gggccgcgtt cgtcgttctg tcggccgccg
     4981 tcggttggct cccgctgcgc ctgagcctgc gacgcatcaa ccagttcgag atgtgagcgc
     5041 gcctatgatc cggacgcctc gcctcgcgcg cgtcttggtg cgctggttcg cgaccgccgc
     5101 tcgcgatttg ccctggcgcc gcacccggaa tccgtacgcc gtctggattg cggaggtcat
     5161 gctacaacag acaactgtcg cggctgtggt tccccattac acccgctgga tgcgcgagtt
     5221 gccgacactt ggcgccctgg ccaccgcctc gccggaacgg gtctaccggc tctgggaagg
     5281 cctgggttat taccgtcgcg cagataacct gctgcgtgcg gcctggatca ttgcggcgcg
     5341 tccagacgag ctctttcccc gtcgttttgc cgagaggctc gcgctgcccg ggctcgagag
     5401 cgacgcggcg ccgtggcgac agatgttgcg tcgcgcgatc cggcgcggct tcctcaaaag
     5461 ctcgattgcg ctcgtgttcc ttgcaggcgg gttcttcgtc gtggaagcgc tgttgcgcgc
     5521 gccgcgctat tgttggcgtt agaactcgaa aatctcgttc atctgaccgc actggggaca
     5581 gcgggaaagg tcgacatccg ggtcgtcggt atagacatag ccgcaacggc cgcaacggaa
     5641 gatgtgatct tcggtgcctg tggggccgaa gctgcgtcgc cgcagttcgt agtaaacggc
     5701 gatggcagac actccggtca ggaggacaac gacgtaggtc aggagaaggt gatcgactgt
     5761 catggggcgt tagtggcggg cggaaccgcc tgccattgga acatcatctg tccccaggtc
     5821 agggacgatg cgcccgatgc cgggttggcc tgttcgaatt gggcggcgcg cgccaattcc
     5881 agcgcgcgtt ggtcggcggc cggcagcccg ctgcgggtca gcagattggc ggcgaggacg
     5941 ctgccgttgc ggtcaaccac cagttgcacg gtcgttgcgg ccagaacgtc gttgtagggc
     6001 cagctcggca actcggggct gtcgagcctc cggcgccggg ccaggtcgcc ctcgacgcgg
     6061 acggtcgatt ggggcggagc ggatgacgac gaggccgagg tggcgccgag aaacggcgtt
     6121 gccgcggggg cggccggcag ggggaatcgg accggctcag tctccgggag aattgggagg
     6181 gtggccaggc ggctcacatc ttgagtcaac cagcggggag gctcggtcca gtgtcgcagg
     6241 ccgctctcca tgcgcgggag ttgaagccag acaggccccg agaagcctcg ggcgctcaca
     6301 agggcgatct ccgccggatc gctcagccac atcctggatt cgagctgcgc ctgcgagatg
     6361 ggttgggtga tgagacgaag tcgtgcgagc cgttgtgcgg gcgtcgcgga cagtgggggt
     6421 cgtggagaga gcaccaggat gatgatcact tgcagcagga aaacgccggc gaccgaaccc
     6481 caccatcggg ctcgatccca ccgcttgaat ccatgcggcg cgtggggatt catggattgc
     6541 ctggaggatg gtcgatctcg agtggaaaca gaggggggcg agtgccgatt acgatgtcgc
     6601 gaatccccgc ctgtcgggcc agggctgcca ggcgcacgat ggcctcgtgc ttgaccgagg
     6661 cgtcagcctg aacgagcagg gagagggggt gccggctctg ctcggatttc gccgcgaggc
     6721 gcgccttcaa ctcctcatcc ccaatcacct ggtgctcgaa ataaaccaac tcgtgctgat
     6781 ccacggtcac gacgagcacc ggcccccgcg gggccggttc atcctcgagc gccaccgacg
     6841 ggagctggat gcgcaatccc ggtcccggcg cgatcgaggt ctgcagcacc aggaagagca
     6901 ccagaatgaa gagaacaccc gcgatcggcg cgacgtccag gcgtccggta aagggcttga
     6961 ggttgcgttg gtatctcatg ggccagaggc ggcagccacg gctcgaggcg cggcgtgccg
     7021 caggcgagcg ttcagagctt ctgaagatcg gtcaggaggc tgacaatctc ggacgacgct
     7081 ttctccatgt cgacgacgat ttcgttgacc cggctgacga ggtagttgta gccggcgtaa
     7141 caggggatgg ccaccgccaa acccgccccg gtgcagacga gcgcctgcca gacgccttcg
     7201 gccagcagtg tggccggcgc ggacaaaccc tgggtttgga ggacgcggaa aacctcgatg
     7261 aaccctagaa ccgtgccgag caaccccatg atcggggcga tctggccgat tgtcgccagg
     7321 agcgccagct tctcctcgag ccggggcact tcgactagtc ccgcgtcctc gagcgcctcg
     7381 cgcacgccct cccggccgcg ttcccgattg agaatagccg tcttgaccag gcgcgccacc
     7441 gggcccgggg tggcgtcgca gatcgagatg gcctcgacga cattgtcgcg cttgagaacg
     7501 ttgcgcacgc cgttgatgaa gtcgagggtg ttgatctgct cgcgatggta gtgcagcaaa
     7561 cgctcgatga acacggcgac ggcaacggcc ccggcaacca ggaggagcca catcatcgaa
     7621 ccgccatgac tgagcaggtc gggtaacatc gccttcttct accgagacgg aggccggatc
     7681 ggcaatcttc aaaacacgcg atccccgacg cgggggatgg gcccagaact caattgcgcc
     7741 aacgggcggc tctccctaat ctcccgcgtc cagagggccc ggcgcaggcg ccgcgccccg
     7801 atgcgccatg aatgtgactc tcaattggct gaagacatac gtcgatttcg attggtccgc
     7861 ggacgaactc gcggagcgat tgaccatgct ggggctcgaa gtggaagggg tcaatcgtct
     7921 ggccggcgag ttcgagggca ttgtcgtagc ccaggtgctc acccgggaca aacaccccaa
     7981 cgccgacaag ctctcgctct gccgcgtgtt cgacggacag ggggaacgcc aaatcgtctg
     8041 cggagctcag aatttccagg ctggtgacaa ggtgcctctg atcttgccgg gccactccct
     8101 gccttcgaag cccgacgagc ccccgttcac gatcaaggtc ggcaaaatcc ggggcgtcga
     8161 gtcacacggc atgctctgct cccccaaaga actcgggttg gccgaggatg cgctaggatt
     8221 gatgatcctg cgggcggatg cccccgtcgg gcagccgttt gccgcctacc tgggccgcac
     8281 gccgggcgat gtggtctacg acctcgaggt cacacccaac cgtccggatc taaacagcgt
     8341 cgtcggcatc gcgcgcgaaa tcgccgctct caccggccat ccattgcgcc tgccggcgct
     8401 gacactgccc tcggcgccgt ccggcgccgt tggggccgcc gatctggttg ccgtgcgcat
     8461 tgaagagccc gacctctgcc cgcgctacac cgcccgcgtg ctgcgcgggg tgaaggtcgg
     8521 gcccagtcca gactggatgc gggctcttct cgagcgtgtg ggtcagcgga gcatcaacaa
     8581 cgtggtcgat gccacgaact tcgtgatgct cgaaatcggg cagccgctcc atgccttcga
     8641 ctaccacctc atcgcccgcg gtcccgcgac ctccggcgag tccgtatcgg gctccgacgc
     8701 agggggcgcg actccgacga tcgtcgtccg ccgggcccgg gccggcgagg cctttgtcac
     8761 cctggacggt cagaaccatt gtctgaccga ggagatgctc ctcattgccg atgaagagaa
     8821 aggcatcgca ctggcgggtg tcatgggagg acaaaactcg gagatcaacg agcagacgat
     8881 cgacgtgctg ctcgagagcg cctgcttcaa gccaaccaac atccggcgaa cctcgaaggc
     8941 cctgggattg cggaccgacg cttcgtaccg cttcgagcgc ggggtggacg cggccatgac
     9001 ggattgggcc agccagcgct gtgcgcagtt gattctcgag gctgccgggg gctgcctggc
     9061 cgccggagtt gtcgacgcct acccgcgtcc ggtcgagccg cggcagatca cattgcggca
     9121 ccacaaggtg aacgaactgc tcggcatcga gatcctcgcg gcggacatcg agtattatct
     9181 tggccaactc gggctcaagg ttgtcggacg caaaccccgg ccggtgggtg actcggatgc
     9241 tgccgagccg gtcacctttg ccattccaac gttccgcccc gacctcaaac gtgaagcgga
     9301 tctcatcgag gaggtgtgtc ggctccatgg cgtcgacaag attcccgccg cgctgcctcg
     9361 aggcgctcga ggcgccagcg aatacgatcc gatccacgac cagcttgccc tggcgcggca
     9421 gctcctcagc ggcctgggtc tctacgaggc acaagggcag acgctcattg cggactccgc
     9481 cgctgcgttg gtcgaggcgg cctcgccagg cgccgatgcc cgacggcccg cgggcatcgt
     9541 ggcgctggcc aatccgctca gcagcgacat gaatgtcctg cgcccaagcc tgttgccggg
     9601 gctcttggag tcgctcaagc acaatctcag ccgccagaac caggatgtcg ccctcttcga
     9661 agtgggccgc gtctttctcc actccgacga cggcgtcggc gagcacaggc ggatcggttt
     9721 ggccctgacc ggacgtcgtc agcccacgtt ttggagcggc acggatcgcg atgccaagtt
     9781 cgatgtctcc gacctgaagg gactggtcga ggagttcctc gaacagtacg ggctgcgcgg
     9841 tgtcagctac gcccggacgc tcgacccccc cccgctcttc gtcgagtccg ccgccatcct
     9901 gctcgggggc aagctgcccc tgggcgtctt ggggcaactc gctccggccc tggccaagcg
     9961 ctatgacctg cgcgaccctg tgtttctcgc cgaactagac ctcgcgcaat tgctagtgcg
    10021 ccgcaacacg gccaagggct tcaaagcgtt gccggcttat ccggccatcc ggcgcgacct
    10081 ggcgatcgtg gtgcctcaga gcgtcacgca cgaggcggtg cttgcggccg tcaagcaggc
    10141 caaagcccag aacctcgagt cggtcgaact gttcgacgtc tatcaaggca agaagatcgc
    10201 gtccgggcag aagagtctcg cgtatgcgtt cacctaccgc agcgcggcgc gcaccttgac
    10261 cgatgccgag gtcaatgccg cgca
//
LOCUS       DAOQRO010000085        10248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5831_length_10248_cov_5.051604, whole genome shotgun sequence.
ACCESSION   DAOQRO010000085 DAOQRO010000000
VERSION     DAOQRO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5831_length_10248_cov_5.051604"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="PKK20_04775"
     CDS             <1..1766
                     /locus_tag="PKK20_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HNU99233.1"
                     /translation="SVGIVDVPGHEDFVKTMVAGVGSIDLALLVVAADDGWMPQTEEH
                     LQILDYLGVTRAVVALTKSDLVGNNEFEAAAAVREQLRHSPFAQAPLVPTSVVTGRGL
                     DELKTALARALAHTEAQPDLGKPRLPVDRVFTLRGIGTVVTGTLTGGRLTRGQAAVLQ
                     PGGVPCRIRSLQNHRTEIETGEPGMRTAVNLADVEPRSDAHPEGVGRGDVLTLSALGG
                     PSATADVWIQRSSRLAAGASPAARPLKDRTRVRIHFGSANAAATLFFQGGRDLLAGQS
                     VLAQARFESPVFMMLGDRFIVRDWSEQVTLAGGLVLDPDGDRKRWQAKPRVALLEARA
                     SAPADIGVWLATQLERDRLVRRDALLKKARCSDVEVAAALGRLAQDGAVILFDELAVH
                     ASWWADLKRRAAERINLEHRARPERLGLALSELAAALEDDVSLPGAFEVLVADLAEGE
                     FRQVGTVIQRRTHRPDLPQRLKAAGAFVRQALAEKPLEPPSRKHLASDQPLQQALQFL
                     LDTGQAVEISADIVLSAEGYADALARIKAHLQRTGAATVSDLRQALGSSRRIMVPLLE
                     KLDREGVTLRQGDLRRLGPRV"
     gene            1951..2664
                     /locus_tag="PKK20_04780"
     CDS             1951..2664
                     /locus_tag="PKK20_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNU99234.1"
                     /translation="MKLLPQFFHSSLGKKYIMAITGLGMLAFVAVHLTGNLQIFLGPE
                     VLDAYAHGLHRAEELLWVIRVCLLVFIGLHISMAVVLTLGNRAARPIPYERSRLPGAS
                     HAARTMIWSGSMVAVFVAYHLLHHSAKAQVSLTGHDFATFQTTLADGTTCHDVFRMVV
                     AGFSQPVVSVFYIVGVGLLCHHLSHGIEAAFQSLGLRNRVWDAAVRKLASGAAWLLFV
                     GYASIPVAVACGYGREALN"
     gene            2666..4582
                     /locus_tag="PKK20_04785"
     CDS             2666..4582
                     /locus_tag="PKK20_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNU99235.1"
                     /translation="MPLDAHIPSGPLESKWERHKSDLGLVHPRHKRKYDVLVVGSGLS
                     GAAAAATLAELGYRVKCFCFQDSARRAHSIAAQGGINAAKNCCNDGDSVSRLFRDTIK
                     GGDFRSREANVYRLAQISAAIIDQCVAQGVPFAREYGGLLENRSFGGAQVSRTFYVRG
                     QTGQQLLLGAYQALSRQISLGNARMFTRTEMLDLVVIDGQAKGIVVRDLVSGEIASHA
                     GDAVVLATGGYANMFYLSTNAKGCNATAVWRAYRRGAVFANPCFTQIHPTCIPVSDEH
                     QCKLTLMSESLRNDGRVWVPRRREDCAKAPGSIPEEDRDYYLERKYPSFGNLAPRDIA
                     SRATKEVCDAGRGVGPGGLGVYLDFGDAIRQMGIERMRERYGNLFEMYERIRGENAYE
                     VPMRMYPAMHYTMGGLWVDYHLMSNLQGLFVIGEANFSDHGANRLGASGLMQGLADGY
                     FILPYTIGHYFASGRPSKAPANHPGFAAAETEVKARLARLLSVQGKRTVTSFHRELGR
                     LLWNDCGMTRSEAGLREALRRIPELRAEFWGDVKVSGSGGELNQDLERANQVADYLEL
                     SELVCRDALMRRESCGGHFRTESVTEDGEAKRDDAQFAFVAAWEFRGEDREPMLHKEP
                     LEYEEARMSTRSYR"
     gene            4582..5337
                     /locus_tag="PKK20_04790"
     CDS             4582..5337
                     /locus_tag="PKK20_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNU99236.1"
                     /translation="MHLSLRVWRQKSASAFGRFETYEARNVSADMSLLEMLDEVNEDL
                     IASGGEPIAFDSDCREGICGACSVVIDGVPHGSRHGTTACQLHLRHFKDGDTITLEPW
                     RARPFPVIKDLVVDRHAFDRIIQAGGYVSVKTGGAPDGNAIPIPKTDVDRAMDAAVCI
                     GCGACVAACKNASAMLFVASKVSHLSLLPQGRVERERRVLAMVRAMDDAGFGACTVTG
                     SCEAVCPKNIRIDVIARMNREYGRALLKAGFVE"
     gene            5467..7290
                     /locus_tag="PKK20_04795"
     CDS             5467..7290
                     /locus_tag="PKK20_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNU99237.1"
                     /translation="MNGKRSTLNAQLRTLNSSIAARIGVLFRSPIALCTGGMKYAGWG
                     EEALPARDACQGPDGLRKMRARMPEQPDSSSALRRAWARLFEVKPLAQLLSETERPGL
                     QLKRTLGPVQLTALGIGAIVGAGIFSTVGTAAAGGANHLGAGPALSLSFLIVAVACCF
                     SALCYAEFASLAPVSGSAYTYAYASLGELVAWIIGWDLILEYAIGNVAVAISWSAYFQ
                     ELLRGFGFLWPEWLGTDYRTASEASALVAQAHAAGQDIGALGESVLHEARAFTLAPRP
                     LGLPIIFNLPAVLAVAAITWILVRGIRESARFNTSMVVLKLGVIVFFLAVGAFYVKPD
                     NWTPFAPHGTEGVLVAAAIIFFAYIGFDAVSTAAEEARNPQRDLPIGIIASLVACTVV
                     YVVVAIVLTGLAPYKTLGTADPLASAFSSVGLSPRALSWVAGVISAGALCANTSVLIA
                     FQLGQPRIFFAMARDGLLPRWAARVHPRFRTPHVTTIITGVFVAFFAGITNIKEMVEL
                     TNIGTLFAFILVAVGILVLRRKDPDRTRPFRTPWVPWVPLAAIACCAVLMAMLPGITW
                     GRFGLWMVAGLALYFIYGFRQSRLRRLQVGGAQAAQRRGPI"
     gene            complement(7317..8426)
                     /locus_tag="PKK20_04800"
     CDS             complement(7317..8426)
                     /locus_tag="PKK20_04800"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNU99238.1"
                     /translation="MRTQSLYRALALALICTVPGNAVAGESAPSSTGAAPTPDAAVLV
                     VRNQGFFPVALRLADGRLAAVLRGGAPHIGLAGRLDIVFSTDDGRTWTAPSLVVDGPL
                     DDRNPAFGQAADGSLVVGYWECGYYDAAGQWAPNAPTAKARTLATRSTDGGKTWTTPE
                     PIDLPGLKFGSPYGRILTMPDQTMLMNVYGVIEGGSHDSSLLFCSKDQGKTWMRHSVI
                     AADGFNETSLARLNDGTLVAALRHDQRAGGPGENVWVASSKDNGLTWSAPISLTPDRV
                     HPADLAVLRDGRLALVAGDRRNPFGLIGIVGDAQGGFEWAAHRVLLDRLTNGDCGYPS
                     AVALNDGTILVVYYAVGSQSQPDWGVHAGAILWRP"
     gene            complement(8459..9160)
                     /gene="nfi"
                     /locus_tag="PKK20_04805"
     CDS             complement(8459..9160)
                     /gene="nfi"
                     /locus_tag="PKK20_04805"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867233.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HNU99239.1"
                     /translation="MKLPPPRHRWRLSPRRAIAVQRRLAARVQIEPFRGEARFIAGLD
                     AAFSVDGAFCLGAVVLWNMTERRVVEERVARRRLQFPYVPGLLSFREAPALLEALRRL
                     DTPPDVLMCDGHGLAHPRRFGIACHLGVLCDLPAIGCAKSRLVGEHAELARARGASVP
                     LRHKLEIVGSVLRTRPGIRPVYVSVGHRIDLAGAQEIVLACAVRFRLPEPTRLADQLA
                     ARAKQTVSRGPDSMS"
     gene            complement(9362..>10248)
                     /locus_tag="PKK20_04810"
     CDS             complement(9362..>10248)
                     /locus_tag="PKK20_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNU99240.1"
                     /translation="LASTLVAYDFNETEMPFQNAKAPARPTITSDQYNIANTTPSFST
                     DSPTGGAGDYSIEFSVPGQRIVVPDPTTAIQLGDENPDIAGDFTIQAWVKFGSIAARA
                     VIWMNNCPGGAVSFSVLNRKVFVTTLGILDRDSDAPIPDDGGWHHIAVVHENGVAFRF
                     YVDGILKDTKAYTGGVIYTRTDPQFTIGCEGSGNLPYVGKLDRLIVSRGVVPAEELDY
                     RVIPGVDPGAPELTIGTVVEVTWPSLPAGYVLQSTTTIADPESWINVPGTPFAREGTY
                     RFYFPPTAEKTFYRLFKP"
BASE COUNT         1691 a         3128 c         3585 g         1844 t
ORIGIN      
        1 tgtcggtggg tatcgtggac gtgccgggcc acgaggactt cgtgaagacg atggtcgcgg
       61 gcgtaggttc gatcgatctt gcgctgctgg tggtggcggc ggacgacggt tggatgcccc
      121 agaccgagga gcacctgcag attctcgatt accttggcgt cacgcgcgcc gtggtggcgc
      181 tgacaaagag cgatctcgtc ggaaacaacg agttcgaggc ggcggccgcg gtacgcgagc
      241 aactgcgcca cagcccgttt gcccaggcgc cccttgttcc cacatcggtc gtcaccggac
      301 gcggcctcga cgaactcaag acggctcttg cgcgcgccct cgcccacaca gaggctcagc
      361 cggacctcgg caaaccgcgg ctgcctgtgg accgtgtgtt cacactcagg ggcatcggca
      421 cggtcgtcac agggacgctc accggagggc ggctcacacg cggacaggca gcggttctcc
      481 aaccgggcgg ggtgccatgc cgcatccggt cgctgcagaa ccataggact gagatcgaga
      541 ccggcgagcc gggcatgcgc accgccgtga acctggccga tgtcgaaccg cggtcggacg
      601 cgcatccgga gggtgtgggg cggggagacg tactgacgct gagcgcgctg ggcggtccga
      661 gtgccacggc ggatgtgtgg attcagcggt cgtcccggct cgccgccggc gcctctcctg
      721 cagcccgtcc gttaaaggac cgcacgcggg tccggattca tttcggcagc gcgaatgcgg
      781 cggcgacact ttttttccag ggcggacgcg atcttctggc gggccaaagc gtgctggccc
      841 aggcgcgctt cgagtcgccg gtgttcatga tgctgggcga tcggttcatt gtgcgcgact
      901 ggtccgagca ggtgacgctg gccggcggcc tggtgctcga tccggacgga gaccggaaac
      961 gctggcaggc caaaccgcgc gtcgctctgc tcgaggcccg cgcctccgcc ccggccgata
     1021 tcggggtctg gttggccacg caactcgagc gcgatcgtct ggtgcgccgg gacgcgcttt
     1081 tgaaaaaggc gcgttgcagc gatgtcgagg ttgcggccgc gcttggtcga ctggcccagg
     1141 atggcgccgt catcctgttc gacgaactgg ccgtccacgc ctcctggtgg gccgacctga
     1201 agcggcgtgc ggcggaacgg atcaacctcg aacatcgggc ccggccggag cggctcggtc
     1261 tggcgttgag cgaattggcc gcggccctcg aagacgacgt ttctctgccc ggcgcgttcg
     1321 aagtcctggt ggcggatttg gcagagggcg agttccgaca ggtcggcacg gtcattcagc
     1381 ggcgaactca tcggcccgac ctgccgcaaa ggctcaaggc cgccggcgcg ttcgtgcgcc
     1441 aagcgctcgc cgaaaagccg ctcgaaccgc cctcccgaaa gcacctcgcg tccgatcagc
     1501 ccttgcagca ggcgctgcag ttcctgctcg acaccggaca ggccgtcgag atcagcgcgg
     1561 acatcgtgtt gtcggcggag gggtatgcgg atgccttggc gcggatcaag gcgcacctcc
     1621 aacggactgg cgcggcgaca gtcagcgact tgcgacaggc gcttggatcc agccgccgca
     1681 tcatggtgcc gttgctcgag aaactcgatc gcgaaggcgt gacgctgcgc cagggcgatc
     1741 tccgtcggct cggaccgaga gtgtgaccgg aggttgacgc agttcagggg ttggcgttag
     1801 atagcggtcg ttgcataagc aaataacaat cattagaaag atggaacatc aatccagcct
     1861 tcgccggcac gcggcctggg tgcggacgcg tccttgcggg gtgtcggcgc ccggcggggc
     1921 gtgctgcgga aggccggcgg catcgaggcc atgaaattgc tgccgcagtt ctttcattcc
     1981 tcgctgggga aaaagtacat catggcgatc acggggctgg ggatgctggc cttcgtggcg
     2041 gtgcacctga caggcaattt gcagatcttt ctggggccgg aggtcttgga cgcgtacgcg
     2101 cacggcctgc atcgggcgga ggagcttttg tgggtgattc gggtatgttt gctggttttc
     2161 atcgggcttc acatctcgat ggcggtcgtg ttgacgcttg ggaatcgggc ggcgcgtccg
     2221 atcccctacg aacgatcgag gctgcctggc gcgtcacacg ccgcgcgcac gatgatctgg
     2281 agcggttcga tggtggcggt gttcgtggcg taccaccttc tgcatcacag cgcgaaggcg
     2341 caggtgagcc tgacaggcca cgactttgcg acgttccaga ccacgctggc tgacggcaca
     2401 acgtgtcatg acgtgttccg gatggtcgtg gcgggctttt cgcagccggt tgtgtccgtg
     2461 ttttacatcg tcggcgtcgg cctgctctgc catcatttga gccacgggat cgaggctgca
     2521 ttccaatcgt tgggactgcg aaaccgcgtc tgggacgccg cggtccggaa gctggcgtcg
     2581 ggcgcggcct ggctgttgtt cgtgggttac gcgtccatcc ccgtggcggt cgcgtgcggg
     2641 tacggaaggg aagcgctcaa ctgagatgcc gctcgacgcg catattccgt cggggccgct
     2701 cgagagcaag tgggagcggc acaagtccga tctcggactc gtgcatcccc gccacaaacg
     2761 gaagtacgac gtcctggtgg ttggatcggg gctgtcgggg gcggcggcgg cggcgacgct
     2821 ggcggaactt ggttaccggg tgaagtgctt ctgtttccaa gacagcgcgc ggcgcgccca
     2881 cagcattgcg gcccaggggg gcatcaacgc cgccaagaac tgctgcaacg acggcgacag
     2941 cgtgtcgcgg ctgttccggg acacgatcaa gggcggggat tttcggtcgc gcgaggcgaa
     3001 cgtgtatcgg ctggcgcaga tcagcgcggc catcatagac cagtgcgtgg cgcagggtgt
     3061 gccgtttgcc cgcgagtacg ggggtctgct cgagaaccgg tcttttgggg gagcgcaggt
     3121 ttcgcgcacc ttttacgtgc ggggtcagac cggccagcag ttgctgctcg gggcctacca
     3181 ggctttgagc cggcagatca gcctgggcaa tgcacgcatg tttacgcgga ctgagatgct
     3241 cgatctggtg gtgatcgacg gccaggccaa ggggattgtg gtgcgcgacc tggtatcggg
     3301 tgagattgca tcgcacgccg gggacgcggt ggtgctggcg acggggggct atgcgaacat
     3361 gttctacctg tcgaccaacg cgaagggctg caatgcgacg gcggtctggc gggcctacag
     3421 gcgcggcgcc gtcttcgcga atccatgttt tacgcagatt catccgacgt gcatcccggt
     3481 cagcgacgag catcaatgca agctgacctt gatgtcggaa tcgcttcgca acgacgggcg
     3541 ggtttgggtc cccaggcgca gggaagactg cgcgaaggcg ccggggtcga ttccggagga
     3601 ggaccgcgac tactacctgg aacggaagta tccgagcttc ggcaacctgg ccccgcgtga
     3661 cattgcgtcc cgggcgacca aggaggtgtg cgatgcgggc cggggggtgg gtccgggagg
     3721 gttgggtgtg tacctggatt tcggcgacgc gatccggcaa atggggatcg agaggatgcg
     3781 ggagcggtat gggaatctgt tcgagatgta cgagcgcatt cggggcgaga atgcgtacga
     3841 ggtgcccatg cggatgtacc cggcgatgca ttacacgatg ggggggctct gggtcgacta
     3901 ccacctcatg agcaacctgc agggcctgtt cgtcatcggg gaggcgaact tctccgatca
     3961 cggggcgaac cggctggggg cgagcggact gatgcagggc cttgcggacg gttatttcat
     4021 cctgccgtac acgatcggcc actacttcgc atccggtcgc ccgtccaagg cgccggccaa
     4081 tcacccgggt ttcgccgccg ccgaaaccga ggtcaaggcg cggctggcgc gcctgctgtc
     4141 cgtgcaaggc aaacgcaccg tgacctcgtt tcaccgggag cttgggcggc tgctgtggaa
     4201 cgactgcggc atgacgcgga gcgaggcggg tctgcgcgag gccctccgcc gcatcccgga
     4261 gttgcgcgcc gagttctggg gcgacgtcaa ggtgagcggg agtggaggcg aattgaatca
     4321 ggatctcgag cgggcgaatc aggtggcgga ttatctcgag ttgagcgaat tggtgtgtcg
     4381 cgacgcgctg atgcgacgcg agtcgtgcgg cggccatttc cggacggaat cggtgaccga
     4441 ggatggggag gcgaagcggg acgatgcgca gtttgcgttc gtggcggcgt gggagtttcg
     4501 gggggaggac cgggaaccga tgttgcacaa ggagcccctc gagtacgagg aggcgcggat
     4561 gagcacgagg agctacaggt gatgcatctg agcttgagag tctggcgcca gaagagcgcc
     4621 tcggccttcg ggcgtttcga gacctatgaa gcgcgcaacg tctccgctga catgtcgttg
     4681 ctcgagatgc tcgacgaggt caatgaggac ttgattgcca gcggcggcga gccgatcgcg
     4741 ttcgattccg attgtcggga ggggatttgc ggggcctgtt cggtggtgat cgacggcgtc
     4801 ccgcacggca gccgccacgg aacaaccgcc tgccagcttc acctccgcca cttcaaggac
     4861 ggcgacacga tcacgctcga accgtggcgg gcacgtccgt tccccgtgat caaagacctg
     4921 gtggtcgatc ggcatgcctt tgaccgcatc attcaggctg gcgggtatgt ttcggtaaaa
     4981 accggaggtg cgccggatgg aaacgccatc ccgattccca agacggacgt cgatcgggcg
     5041 atggacgccg cggtgtgcat cgggtgcgga gcgtgcgtgg cggcgtgcaa gaatgcctcg
     5101 gccatgctct ttgtcgcctc gaaggtgtct catctgagcc tcctgcccca ggggcgagtc
     5161 gagcgcgagc ggcgtgtcct cgcgatggtg cgcgcgatgg acgatgccgg ctttggcgcc
     5221 tgcacggtga cgggatcgtg cgaggcggtt tgcccgaaga acatccggat cgatgtcatc
     5281 gctcggatga atcgcgagta cggccgcgcc ctccttaaag cggggttcgt agaataggcg
     5341 acaaaattcc cctcacccgt cccgcgttcg ctggccaccc tctcccgtcc ggcgggagag
     5401 ggatggagcg atgggtctgc ttcgtgggag agcgttgaca ttcaacggtt gaatgcggac
     5461 gggcccatga acgggaaacg ttcaacgctc aacgctcaac tccgaactct gaactcgtcg
     5521 atcgcagccc gcataggagt cctctttcga agtccgatcg ccctgtgcac gggtgggatg
     5581 aaatatgccg gctgggggga ggaggcgctc ccggcacggg acgcttgcca aggcccggat
     5641 ggcttgcgca agatgcgcgc gcgcatgcca gaacaaccgg acagttcgtc ggcgctccgc
     5701 cgggcctggg ctcggctttt cgaggtcaag cctctggcac agcttctgag cgaaacggaa
     5761 cgccccggtc tgcaactcaa acgcacgctc ggtccagtgc aactgaccgc gctgggcatt
     5821 ggcgcgatcg tgggggcggg catcttctcg acggtgggca cggcggcggc gggcggcgcc
     5881 aaccatctgg gcgccggtcc ggctctgtcg ctttcgtttc tgatcgtggc ggtggcatgc
     5941 tgtttctcgg cgctctgcta cgcggagttt gcgtcgttgg cgccggtttc cggttcggcc
     6001 tacacgtatg cgtacgcgtc gctgggcgaa cttgtcgcct ggattatcgg atgggacctg
     6061 atcctcgaat acgcgatcgg gaacgtggcg gtcgccattt cgtggtcggc ctacttccag
     6121 gagttgctgc gcggtttcgg ctttctatgg cccgagtggc tgggcacgga ctaccggact
     6181 gcgtcggaag catcggccct cgtggcccaa gcccatgcgg cgggccagga catcggcgcg
     6241 ctgggcgagt cggtcctgca cgaagcgcgc gcgttcacgc tggcgccgcg cccgctcggt
     6301 ttgcccatca tcttcaacct gcccgccgtt cttgcggtag cggcgatcac atggattctg
     6361 gtgcggggca tccgggagag cgcgcgcttc aacacctcga tggttgtgct gaaactgggc
     6421 gtcattgtgt ttttccttgc ggtgggcgcg ttctacgtca agcccgacaa ctggactccg
     6481 ttcgccccgc acggcacaga gggggttctg gtggcggcgg cgatcatttt ctttgcctac
     6541 atcgggtttg acgccgtatc gaccgcagcg gaggaggcgc gcaacccgca acgcgacctg
     6601 ccgatcggga ttatcgccag cctggtggcg tgcaccgtgg tgtacgtggt cgtggcgatt
     6661 gtgctgacgg gacttgcacc ctacaagaca ctgggaacgg ctgatccgct cgcgtcggcc
     6721 ttctcctcgg tcggcctcag cccgcgcgcg ctttcgtggg ttgcaggcgt gatctcggcc
     6781 ggcgccctct gcgcgaacac gtcggtgctt atcgcgtttc agcttggcca gccccgcatc
     6841 ttcttcgcga tggcccgcga cggtctgctg ccgcgctggg cggcgcgcgt tcacccgcgc
     6901 tttcgcacgc cacatgtcac gaccatcatc accggcgttt tcgtggcgtt ctttgccggc
     6961 atcaccaaca tcaaggagat ggtggaattg accaacatcg gcacgctgtt cgccttcatc
     7021 ctggttgccg tcggcatcct ggtgctgcgc cggaaagacc ccgatcggac gcgtccgttc
     7081 cgcacgccgt gggtgccgtg ggtgccgttg gcggcgatcg cgtgttgcgc cgtgctgatg
     7141 gcgatgttgc cggggatcac ctggggccgg ttcggtctct ggatggtggc gggcctggcg
     7201 ctgtacttca tctacggctt ccgccagagc cgactgcggc gcctccaagt cggaggcgcg
     7261 caggccgcgc agcgacgggg gcccatctga cgcgcgagcg caggccccct cgaccgtcaa
     7321 gggcgccaga gaatcgcgcc ggcgtgcacg ccccagtcgg gctggctctg gctgccgacc
     7381 gcgtaataca ccaccaggat tgtcccgtcg ttcaacgcca ccgcgctcgg gtagccgcaa
     7441 tcgccgttgg tcagacgatc cagaagcacg cggtgcgccg cccactcgaa tccgccctgc
     7501 gcgtcaccta cgatgccgat caacccgaac ggattcctcc ggtcgccggc caccaacgcg
     7561 agccgcccgt cccgcaagac cgccaggtca gccggatgca cgcggtccgg cgtcagcgag
     7621 atcggagccg accaggtcag gccattatct ttgctcgatg caacccacac gttctcgccc
     7681 gggccacccg ccctctgatc gtggcgcagc gcggcgacca gcgtgccgtc gttcaagcgg
     7741 gcgaggctgg tctcgttgaa gccgtccgcc gcaatcacgc tgtgccgcat ccaggtcttg
     7801 ccttggtctt tcgagcaaaa cagcagactc gagtcgtggc tgcctccctc gatgacgccg
     7861 tagacattca tcaacatcgt ctggtccggc atggtcagaa tgcgtccgta cgggctgccg
     7921 aacttgagcc ccggcagatc gatcggctcg ggtgtggtcc aggtcttgcc gccgtctgtc
     7981 gagcgggtgg ccagcgtccg cgccttggcg gtcggcgcgt tgggcgccca ctgcccggcc
     8041 gcgtcgtagt agccgcattc ccaataaccc acgacaaggg agccgtccgc cgcctgcccg
     8101 aaggcggggt tccgatcatc caacggaccg tccacaacga gcgatggcgc cgtccaagtc
     8161 cggccatcgt ccgtcgaaaa cacgatgtcc aggcgacctg ccagaccgat gtggggcgcc
     8221 ccgccgcgca acacggccgc cagccgtccg tccgccaacc gcaaggcaac tgggaagaag
     8281 ccctggttcc tgacgacgag gacggcggca tctggcgttg gcgctgcgcc tgtcgaggac
     8341 ggtgccgatt cgccggcaac cgcgtttccc ggcactgtgc agatcagagc gagcgcgaga
     8401 gctcgataga gcgattgggt tctcatgggc aggaacatac cactcgaagt gcgcccggtc
     8461 acgacattga atctggtccg cgggaaacgg tctgtttcgc tcgggcagcc agttgatcgg
     8521 ccagacgcgt cggctcgggc aatcggaagc ggacggcgca agccagaacg atctcctgcg
     8581 cgccggcgag gtcgatgcga tggccgacgc tgacatagac ggggcgaatc ccgggccggg
     8641 ttctcaagac cgagccaacg atctcgagtt tgtgccgcaa cggaaccgag gcgccgcgcg
     8701 cgcgtgcgag ttcggcgtgt tcgcccacga gccggctctt ggcgcaaccg atcgcgggca
     8761 gatcgcagag aactcccaaa tgacatgcga tgccaaagcg gcgcggatgg gccagtccat
     8821 gaccatcgca catgagcacg tcgggcggcg tgtcgagccg acgcagcgct tcgagcaagg
     8881 ccggcgcctc gcgaaaggaa agcaacccgg gcacatacgg aaactgcagc cggcgccgcg
     8941 ccacgcgctc ttcgaccacg cgccgctccg tcatgttcca caggacgacc gcgcccagac
     9001 agaaggcgcc gtcaactgaa aatgcggcat cgagaccggc gatgaagcgg gcttctcccc
     9061 tgaacggttc gatctggaca cgcgcggcga ggcgccgttg aacggcgatc gcacgacggg
     9121 gtgagagccg ccagcgatgc cgtggcgggg ggagtttcac gcgaacgaat gaccggccag
     9181 gcgggccaga acgtgcgtgt gcttcagccc gctttggtgt ggtcaagcac tgaggaccat
     9241 gcctggaaag cggcggttca gcgctttcct gtgacgattg ttcgagaatg cggggttccc
     9301 gcggccctga cgggccgcgg gaacccggga atggacaggc cgtgacggcc tgtgcagcgc
     9361 cttacggctt gaacagccgg tagaaggtct tctcggcagt gggcgggaag tagaaccggt
     9421 aggtgccctc cctggcaaac ggcgtgccag ggacgttgat ccagctttcg gggtccgcga
     9481 tggtcgttgt ggactgcagg acatagccgg ccggcaggga aggccaggtc acttcgacca
     9541 cggtgccaat cgtcagctca ggcgcgcccg gatcgacgcc ggggatgacg cggtaatcca
     9601 gttcctctgc cggaaccaca ccgcggctca cgatcaagcg gtcaagcttg cccacgtacg
     9661 gcaggttgcc cgagccttcg cagccgatag tgaactgcgg gtcggtgcgc gtgtagatca
     9721 cgccccctgt gtaggctttg gtgtccttca aaatgccgtc cacgtagaac cggaacgcga
     9781 cgccattctc atggacaacg gcgatgtgat gccatccgcc atcgtcgggg atcggggcgt
     9841 ccgaatcgcg gtcgagaata ccgagggtgg tgacgaacac tttgcgattc aacacggaga
     9901 aggagacagc gccgccgggg cagttgttca tccagatcac tgcgcgcgcc gcgatgctgc
     9961 cgaacttgac ccaggcctgg atcgtgaaat cgccggcgat gtccgggttc tcgtccccga
    10021 gctggatcgc cgtggtggga tcgggcacca cgatgcgctg gcccgggacg gagaactcga
    10081 ttgagtaatc gcccgcgccg ccggttggtg agtctgtcga gaacgacggc gtcgtgttgg
    10141 ctatgttgta ttggtccgac gtaatggtcg gccgggccgg ggctttggcg ttctgaaacg
    10201 gcatctcggt ttcgttgaag tcgtaggcga cgagcgtgct ggcaagga
//
LOCUS       DAOQRO010000086        10161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5905_length_10161_cov_5.022462, whole genome shotgun sequence.
ACCESSION   DAOQRO010000086 DAOQRO010000000
VERSION     DAOQRO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5905_length_10161_cov_5.022462"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKK20_04815"
     CDS             <1..337
                     /locus_tag="PKK20_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008607794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99241.1"
                     /translation="DANGWALNAKAKMNVPFGQSLTGIAKLPPGSSRDLRDPFTEKKS
                     CWPKFIVLALILYVAYTVLNHLGYVHQWTHGRIGIEKRLTTAQSATPANAAKPAADAA
                     AAAAPSAGQ"
     gene            711..3395
                     /locus_tag="PKK20_04820"
     CDS             711..3395
                     /locus_tag="PKK20_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNU99242.1"
                     /translation="MRTFLLSGLTCAVGVGVAVLVSGCARHTTPWSGPPLSAPSSVGR
                     VARNRYQTPSGQVLTPAGRQVELPGMRPQALALSPDGTRLATAGRNHTLVLIDAATGR
                     VKQTVQLSARRITRTSTNTLSGQLSFTGLVFAPDGRRIYLSNTGGSVWAFPVDEQGEA
                     GAPVVLDVPDAKAPKQAHEIPTGLAVSEDGRRLYVVGNLGDRLHEMDPVSGKVLRSWD
                     TGVAPQDVVLARGKAYVSNRGGRRPGEDDLTAPAGKGTAVRVDAVRHIANEGSVTVID
                     LAEGKVIREIVVGLHASALAVSPDGAYVVVANTGSDTLSVIDTRTDRVIEKIWARQTP
                     ADLFGAQPNAMAFDPTGRRLYVCNGTQNAVAVVEFEPEENASKVIGLIPVGWFPGAIQ
                     YDATRNTLCVANMKGLGALKVFKPDEPIRLSSKTFWGMVSLVPVPTPDGLAALTRSAL
                     ENMHYPRLAQALRPARPRQPARPVPERVGEPSVFKHVIYVIKENRSYDQVLGDMPEGN
                     GDPTLCTFGEQYTPNQHKIARDFVLLDNTYCSSIQSSDGHQWIGSAMANEYTERQATS
                     GTPRSYSGGKSDESGDALSWASSGFLWDNALAHGKTLRNYGEWMHSESGWKDRKRKGS
                     ATWSDFWREHQTGAGELELRCRPVIESLRPHSPTNTVGYDARVPDQMRADFFIRELRR
                     FETNGGFPDLTILFLPNDHTAGTSVNYPKPGSMIADNDLALGRLIEALSHSRFWPETC
                     LFAIEDDPQAGWDHVSGYRTTCYVVSPFTKRRQTVSTQYNHACLIRTIELILGLPPMN
                     QLDATATPMFDCFTAVPDLTPYTAVPNRVPLDEMNPDPNRVSHPILRQDAIVSSQLPL
                     DEVDQCPEDVLNRILWRAMKGPDTPYPEWAVKMVEDDD"
     gene            complement(3392..4432)
                     /gene="asnA"
                     /locus_tag="PKK20_04825"
     CDS             complement(3392..4432)
                     /gene="asnA"
                     /locus_tag="PKK20_04825"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNU99243.1"
                     /translation="MNHNLILPRNYRPLLGVKETERAIKDLKDFFELNLATELNLSRV
                     TAPLFVKGGTGINDDLNGIEQPVAFAVKGMPGVRAEIVQSLAKWKRMQLADLGFEPGT
                     GLYTDMNAVRPDETLDNTHSIYVDQWDWERVIAPDERTVEFLKKIVRRIYAILKRTEH
                     FVFERHPVFRPQLPEEITFIHAADLRMRYPNLTPRLREQRLLRECGAAFIIGIGGDLG
                     DGSIHDGRAPDYDDWSTPTGRGRQGLNGDILVWNPVLRKAFELSSMGIRVDKKALVRQ
                     LRICGCEERRHLVWHRRLLDDGMPLSVGGGIGQSRLCMYFLRKAHVGETQASLWPEEM
                     IATCRRHNMPLL"
     gene            complement(5528..6427)
                     /locus_tag="PKK20_04830"
     CDS             complement(5528..6427)
                     /locus_tag="PKK20_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNU99244.1"
                     /translation="MSVLIVGSTALDSIKTPRRACAKLLGGSASHASVAASFFAPVKM
                     VGIVGSDFPTRYIALYRRHGIDLAGLQRVEGRTFHWSGEYEVNMNNRRTLTTELGVFE
                     QFRPALPPTHQTTPYVLLANIAPALQHHVLDQMRRRRFVVADTMDLWLRIARADLLRL
                     LRRVDALVLNDSEARDLAGTDNLFTAAARIRRMGPRYVVIKKGEHGAVLSCPDSLFVC
                     PAYPLARVTDPTGAGDSFAGGLVGYLASVKGPVERHLRRAMVYGSAVASFCCEGFGLS
                     RTDRITRADIDARVAELERLARF"
     gene            complement(6444..7115)
                     /gene="thiE"
                     /locus_tag="PKK20_04835"
     CDS             complement(6444..7115)
                     /gene="thiE"
                     /locus_tag="PKK20_04835"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNU99245.1"
                     /translation="MQPIDRCLLYGFVDTAYLRGRSPERVARDLCEGGADLIQLRAKG
                     VPSAEVRRLAERLQPIAREAGVGLVINDFPEVALDVGAPLCHLGQEDFFDHGHTHVRE
                     LFGQAPGHCVAPGVGLSTHAPAQAQRALTAGPAYIAIGPVFATGTKPTATPVTLEYVR
                     WAAAHVSIPWFAIGGITLDNLDDVLDAGARRVCVVSAILGADDVVSACQAFKQRLVSK
                     ARECP"
     gene            7264..8169
                     /locus_tag="PKK20_04840"
     CDS             7264..8169
                     /locus_tag="PKK20_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNU99246.1"
                     /translation="MDAFALEGLIPAAHTPFRKDGAIDLDRVPDQARHLLRHQLQTVF
                     IAGSTGESHSLTIEERRHLTARWTEVARGTPLRIIVHVGSNCLSDACALAAHAQEAGA
                     SAVAALAPSYFKPRDLDALIACCAAIAVKAPALPFYFYDIPALTGVSLPMPEFLERAS
                     RVIPTLAGIKFTNLDFAQLQLCLDLKSHDIAWGVDEALIGALACGVRSAVGSTYNFAA
                     PIAQRLVDAFHRGDLETARREQLRLVRLVAALARPGYLAAAKYLMERLGVEVGPPRLP
                     LTPLRPEQIQVLEDDLARLGFEGWM"
     gene            complement(8191..8709)
                     /locus_tag="PKK20_04845"
     CDS             complement(8191..8709)
                     /locus_tag="PKK20_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNU99247.1"
                     /translation="MPIYEFACPKCRVIFNFLSKKVNPEHTPKCPKCGNPRLTKQVSR
                     FAAPKGVPEPKGKTDIADDEGPMPDIDEARMERVMAELERDMDHLDENNPRHMAHMMK
                     KMKDIMPPGTMPKEMDVAIKRLEKGEDPEKIEEDMGDVLGDLMGGPEEDDDGYGGMGS
                     GAYSRDSGLYDY"
     gene            9038..10018
                     /gene="selD"
                     /locus_tag="PKK20_04850"
                     /pseudo
     CDS             9038..10018
                     /gene="selD"
                     /locus_tag="PKK20_04850"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
BASE COUNT         1869 a         3286 c         3219 g         1787 t
ORIGIN      
        1 ggatgccaat gggtgggcgc tcaacgcgaa ggccaagatg aacgtgccgt tcggccagtc
       61 tctgaccggc atagcgaagc tgcctccggg ttcctcccga gatttgcgcg atccgttcac
      121 cgaaaagaag agttgctggc ccaaattcat cgttctcgcc ttgatcctgt acgtcgccta
      181 caccgtgctg aaccacctcg gctacgtcca tcaatggacc cacggccgaa tcgggatcga
      241 gaagagactc acaaccgccc agtctgcaac tcctgccaac gccgcaaaac cggcagccga
      301 tgccgccgcc gcggcggcgc cgtcagccgg gcaataggtc gtggcctcac acgtcggact
      361 ccgctccgcc cccccgcgca ctctcgggcg gagaaggcct caatgaccga ttccatgtcg
      421 cgatcgcgac atggaatcgg tatgggtctg gtgcgatggg tgcctggagt tctattcacg
      481 cggatcacgg cgcggctcac gatcatcccc gcccctccgg ccagaccttg gactcatgaa
      541 gccgcaaagg ccggagcggg gacggttcgt ccccggccca cagcacgaaa gaatgcggcg
      601 gttgtaggcc tgatccccgg acccgcgctc cgagcgtcgt caagaaaaga acctctgggt
      661 cgggagcaac tcaggtagac gcacgcgcgc agtccgtgta aaattctcac atgcggacgt
      721 ttcttttgtc tgggctgacc tgcgccgtgg gcgtgggagt tgccgtcctg gtctcgggat
      781 gcgcccgaca cacgaccccg tggtcgggac cgccactgtc agcgccctcg agtgtcgggc
      841 gcgtggcgcg gaaccgctac cagaccccga gcggtcaggt gctgaccccc gcgggcaggc
      901 aggtcgagtt gcctgggatg cgtccccagg cgcttgcgtt gagtcccgac ggcacacggc
      961 ttgccacggc cggcaggaat cacacgctgg tcctgatcga tgcggccacg ggccgggtga
     1021 agcaaacggt tcagctttcg gcccggagga ttacccggac cagcacgaac accctctccg
     1081 gccagttgag tttcacgggg cttgttttcg cgccggacgg acgccggatt tacctttcga
     1141 acaccggcgg gagtgtctgg gcgtttccgg tggatgagca aggcgaggcc ggtgcgccgg
     1201 tggttttgga cgtgccggac gccaaagctc ccaagcaagc gcacgagatt cccaccggac
     1261 tggccgtgtc ggaagatggc cggcggctct atgttgtggg caatctggga gatcggctcc
     1321 acgaaatgga ccccgtgtcg ggaaaagtcc tgcgttcgtg ggacaccggc gtggccccgc
     1381 aagacgtggt gctggcccgc ggcaaggcct acgtcagcaa ccggggcgga cgccgtcctg
     1441 gtgaggatga tctcactgcg cctgcgggca aaggcaccgc ggtgcgggtg gacgcggtgc
     1501 gacacatagc caacgagggt tccgtgacgg ttatcgactt ggcggaaggc aaggtgataa
     1561 gggagatcgt cgttgggctt cacgcgtcgg ctctggcggt ttctcccgat ggcgcgtatg
     1621 tggtggtggc caacacgggc agtgacacac tcagcgtgat tgatacccgg acagatcggg
     1681 tcatcgagaa gatttgggcg cgccagacgc cggccgacct gttcggcgcg cagccgaatg
     1741 cgatggcttt cgacccgacc gggcgacgcc tgtacgtctg taacggcact cagaacgccg
     1801 tcgccgtcgt cgagtttgag cccgaggaaa atgcatccaa ggtcattggg ctcattcccg
     1861 tcggctggtt cccgggggcc atccagtacg atgccacgcg caacacgctc tgtgtcgcga
     1921 acatgaaagg gcttggggct ctgaaggtgt tcaagccgga tgaaccgatc agactcagca
     1981 gcaagacctt ttggggcatg gtgtcgctcg tgcccgttcc gaccccggac gggctggccg
     2041 cgctcacgcg cagcgcgctc gagaacatgc attacccgag gcttgcccag gcgttgcgcc
     2101 cggcgcgtcc gcgccaaccc gcccgtcccg tgcccgagag ggtgggcgag ccgagcgtgt
     2161 tcaaacatgt gatttacgtc atcaaggaaa accggagcta tgatcaggtc ctgggtgaca
     2221 tgcccgaggg caacggcgac ccgacgctct gcaccttcgg cgagcaatac acgccgaatc
     2281 agcacaaaat cgcccgcgac ttcgtcctgc tggacaacac gtactgctcg agcattcaga
     2341 gttccgatgg ccatcaatgg atcgggagcg ccatggccaa cgaatacacc gaacgccagg
     2401 ccacctcagg cacgccccgc agctactccg gcggcaagag cgatgagagc ggagacgcgc
     2461 tgtcatgggc ttcgtccgga ttcctctggg acaacgcgct ggcgcatgga aagacactcc
     2521 gcaattacgg cgaatggatg cactccgagt cgggctggaa ggaccgaaaa cgcaagggga
     2581 gcgccacgtg gagcgacttc tggcgcgagc accagaccgg cgccggcgaa ttggaactcc
     2641 gctgccgtcc ggtgatcgaa tccctgcgtc cgcacagccc caccaacacc gtcggctatg
     2701 atgccagggt gccggaccag atgcgcgccg acttcttcat ccgggaactc cgccgcttcg
     2761 agaccaatgg cgggtttccg gacctgacga ttctcttcct gcccaatgac cacaccgccg
     2821 gaaccagcgt caactatccg aagccgggat cgatgatcgc cgacaacgat ctcgccctgg
     2881 gccggctgat cgaggcgctc agccatagcc ggttttggcc ggagacctgc ctttttgcga
     2941 tcgaggatga tccccaggcg ggttgggatc atgtgagcgg ctaccgcacc acctgctatg
     3001 tggtcagccc cttcaccaaa cgccggcaga ccgtcagcac ccagtacaac catgcctgcc
     3061 tcatccgcac aatcgagctg atcctcggct tgccgcccat gaaccaactg gacgccaccg
     3121 ccacgcccat gtttgattgc ttcaccgcgg tgccggacct gaccccatac accgcggtac
     3181 ccaaccgggt cccgctcgat gaaatgaacc cggacccgaa ccgggtctcc catccgatcc
     3241 tgcgacagga tgccatcgtt tcgagccagt tgcctctgga cgaagtggac caatgcccgg
     3301 aggacgttct caaccgcatc ctctggcgcg cgatgaaagg ccccgacaca ccctatcccg
     3361 aatgggccgt caagatggtc gaggatgacg actagagcag cggcatgttg tgtctgcgac
     3421 aggtcgcgat catctcctcc ggccaaaggc tggcctgggt ttcgccgacg tgcgccttgc
     3481 gtagaaaata catgcagagg cgggattggc cgatgccgcc gcccaccgac agtggcatgc
     3541 cgtcgtcgag cagtcgccga tgccagacca agtgcctccg ctcctcgcac ccgcaaatcc
     3601 tcaactgccg caccagcgcc ttcttgtcga cccggattcc catggacgac agttcgaagg
     3661 cctttcgcaa aacagggttc cagaccagga tatcgccgtt gagaccctgg cgtccgcgtc
     3721 cggtgggtgt cgaccagtca tcgtagtcgg gagcccggcc gtcgtggatc gagccgtcgc
     3781 ccaggtcgcc gccgatgccg atgatgaagg cggcgccgca ttcgcgcagc aaccgttgtt
     3841 cacgcagccg gggcgttaga ttcggatagc gcatccgcag gtccgccgcg tggatgaacg
     3901 tgatctcctc cggcaattgc gggcggaaga cgggatgacg ctcgaagacg aagtgctcgg
     3961 tccgcttgag gatcgcgtag attctgcgca cgatcttctt aaggaactcg acagtgcgtt
     4021 catcgggcgc gatcacacgc tcccagtccc attggtcgac atagatcgag tgggtgttgt
     4081 ccagggtctc gtccggtcga accgcgttca tatcggtgta cagcccagtg ccaggctcga
     4141 agccgaggtc ggcgagctgc atgcgcttcc acttggcgag ggactggacg atctcggcgc
     4201 ggacgcccgg catccctttg acggcgaagg cgacgggttg ctcgatgccg ttgaggtcgt
     4261 cgttaatccc cgtgccgcct ttgacaaaaa gcggggcggt gacccggctc agattgagtt
     4321 cagtcgccag gtttagttca aagaagtcct tgaggtcctt gatcgcgcgc tccgtttcct
     4381 tgaccccgag cagcggcctg tagtttcgag gcaagatcag attgtggttc atgcgtgcca
     4441 ttcaattcgc gcccgccagc ctagcagccg agccgcacgg cggccaaaga aaactagacc
     4501 gaacgatgat cacctcgttc gcccccatga cggtgccccc accgcacaga gacgggcatg
     4561 aaccggaaag agcacagaga gcagtacaaa gcggcgtgat gcggccggca ggatcaccgt
     4621 ctcggtgcca atctgtgttc cgccctgaat cggtgtgggg actgtctgcg cactaaaatc
     4681 cgtgaggttc tccacacaga ttcagggcgg aacacagatg gagagggaga gaggcagtgt
     4741 gtcggccccc ggggggtggt gggtcctaac ccgcatattt cacaccgcga tggtgcacag
     4801 ggcactcaga cttcgcaaga agactcccat gcgggttaga aggatcgagt cctttgcgtt
     4861 ctttatggtt ggttccgcct cggtcacggg ccaacaagcc ggctgcatct gaacgccggg
     4921 atcgcgtgtc ggttcgtaat cggacatcat tggcccggcg tcggtgagct gggacgttcg
     4981 atggtcggcg acgggcgaca atcgagtccg agtcgagagc gccgcgtaac cccggccggc
     5041 ccaaaacgac gcccaatcgg cattggcccc ccttcccggc gccatggatc aaggcgattc
     5101 ccggcacagg gtctggagcg ttggtcgggc acgcgtaata ggcaaacacg cgcgtcgggc
     5161 gacctgcgtg cggcacactt tcgtaataga cttcccggac atcgcccgtg ctggcgcccc
     5221 agacaaccgg gggtacggac ttcaaggttg gcagattcca cggacccgtg aaccgctcgt
     5281 cagcccgtct gcaggaaaag acgccaagaa gaacccccag actcagccgg caaatctcct
     5341 gtcggaacga agagaaagga cctcggaccg tggcaaaaca gcgtccagaa agtgagccga
     5401 gagcctcccc gaacacccgt atgtcgatct gacgccgcgc attagacagc gctcaaattc
     5461 cggacgccga gtcgcgcgtc actgcccgtc tggctgcgtc cgcggtcacg tcgtccttgc
     5521 cccgatctca gaatcgagcc agccgctcga gttcggctac gcgcgcgtcg atgtcggcgc
     5581 gtgtgatgcg gtcggttcga ctcaggccaa agccctcgca acagaaggac gccaccgcgc
     5641 tgccatacac catggcccga cgcaggtgcc gctcgaccgg ccccttgacg cttgccagat
     5701 agcccacgag accccccgcg aacgaatcgc ccgcaccggt gggatcggtg acgcgcgcca
     5761 gcggatacgc cgggcaaaca aagaggctgt ccgggcagga caacaccgcg ccatgctcgc
     5821 ccttcttgat gacgacgtaa cgcggcccca tgcggcgaat ccgcgcggcg gccgtgaaga
     5881 ggttgtccgt gccggccaga tcgcgcgctt cgctgtcgtt caacaccagc gcgtccacgc
     5941 gccgaagcaa gcgcaggaga tcggcgcgcg cgatcctgag ccaaaggtcc atggtatccg
     6001 ccacgacgaa tctgcgccgc cgcatctggt ccagaacatg gtgttggagg gcgggggcga
     6061 tgttcgccag caagacgtac ggagttgttt ggtgggtggg gggcagggcc ggacggaact
     6121 gttcgaacac gcccagctcg gttgtcagcg tgcgtcggtt gttcatgttg acctcgtatt
     6181 ccccagacca gtggaaggtc cggccctcga cgcgctgcaa tccggcgaga tcgatgccgt
     6241 gccgccgata gagcgcgatg tagcgcgtcg ggaagtcact gccaacgatg ccgaccatct
     6301 tgaccggggc gaagaagctg gcggccacgc tggcgtggct tgccgagccg cccagcagct
     6361 ttgcgcaagc gcggcggggc gtcttgatgg agtccagcgc ggtcgatccg acaatgagga
     6421 cactcatggg atggattcgt tcttcacgga cattctcggg ctttcgagac taggcgctgc
     6481 ttgaaggcct gacaagcgct gacaacgtca tccgcgccca aaatggcgga cacgacacag
     6541 acgcgccgag cgcccgcgtc caatacgtca tccagattgt ccaatgtgat gccgccgatg
     6601 gcgaaccagg gaatggacac gtgggcggcg gcccaacgca catactcgag ggttactggc
     6661 gtcgctgtcg gcttggtccc cgtggcaaac accgggccga tggcgatgta ggcgggtccg
     6721 gctgtcagcg cgcgttgcgc ctgggctggc gcgtgcgtgc tcaggccgac accgggggca
     6781 acgcagtggc cgggggcctg gccgaacaac tcgcgcacgt gtgtatggcc gtgatcgaag
     6841 aaatcctcct ggcccaggtg gcacagaggg gcgcccacat ccaaagccac ctccgggaaa
     6901 tcgttgatga ccaaacccac tccggcctct cgcgcgattg gctgaaggcg ttcggccagt
     6961 cgccggacct cggcggatgg cactcctttg gcccgaagct ggatcaggtc cgccccgccc
     7021 tcgcagaggt cacgcgcaac gcgctccggc gaacggccgc gcagataggc ggtgtcgacg
     7081 aagccgtaca acaggcaacg atcgatcggt tgcataatac gttcgtgtag cccgagcgcg
     7141 ccaatcgcga aagcacgaag tcgcggggcg ttggttatcg agagggattc tcggaggaga
     7201 ttcccaaaga ctcccgaagg ggattgcatg gcatcgcgcg tggacgctac catccgatcc
     7261 tcaatggacg cattcgcact cgaaggcctc atcccggccg cgcacacacc cttcaggaaa
     7321 gacggcgcta tcgacctcga tcgcgtcccg gaccaggccc gccacttact ccgccaccaa
     7381 cttcaaaccg tgttcatcgc cggaagcacc ggtgaaagcc actcgctcac catcgaggag
     7441 cgccggcacc tcacggcgcg atggacggag gttgcgcgcg gcacccccct ccgcatcatc
     7501 gtccatgtcg gctcgaattg cctgagcgac gcctgcgctc tggcggcgca cgcgcaggag
     7561 gcgggcgcgt cggcggttgc ggccctcgcg ccgagctact tcaagccgcg cgatcttgac
     7621 gccctgattg cctgttgcgc cgccattgcc gtcaaggcgc cggctttgcc gttctacttt
     7681 tacgacatcc cggcgctcac cggcgtgtcg cttccgatgc cggagtttct cgagcgcgcg
     7741 tcgcgcgtca ttccgactct ggcagggatc aagttcacca acctggattt tgcccaactc
     7801 cagctctgcc tcgatctcaa atcgcacgac attgcctggg gagttgacga agcgctgatc
     7861 ggcgcgctcg cctgcggagt gcgcagcgcc gtgggcagca catacaactt tgccgccccg
     7921 attgcccagc gcctggtcga cgcctttcac cgcggcgatc tcgagaccgc gcgccgcgaa
     7981 cagttgcgtc tggtccggct cgttgccgcg ctggcccggc ccggctacct ggcggccgcc
     8041 aagtatctca tggaacggct cggggtcgag gtcgggccgc cgcgtctgcc tctcaccccg
     8101 ctgcggcccg agcagattca ggttctcgaa gacgatctgg cgcgactcgg tttcgagggg
     8161 tggatgtagc gggcggacgg catctgttcg tcagtagtcg tagagaccgc tgtcgcggct
     8221 gtacgcgccg ctccccatgc ctccgtaccc gtcatcgtcc tcttcgggtc cgcccatgag
     8281 gtccccgagc acatccccca tgtcctcctc gatcttctcg gggtcctcgc ctttctcgag
     8341 ccgtttgatg gcgacgtcca tttccttggg catggtgccc ggcggcatga tgtctttcat
     8401 cttcttcatc atgtgcgcca tgtgccgggg gttgttctcg tccagatgat ccatgtcgcg
     8461 ttccaattca gccatgaccc gctccatccg cgcctcgtcg atgtcgggca tcggcccttc
     8521 gtcgtccgcg atgtccgttt tgcccttggg ctcaggcacg cctttggggg cggcgaaccg
     8581 gctcacctgc ttggtcagcc tggggttgcc acatttgggg catttggggg tgtgctctgg
     8641 attcaccttc ttggacagaa agttgaagat gacccggcac ttgggacagg caaattcgta
     8701 gattggcata accctgaacg acatgccgag tttggaaaac caccggccaa acgtcaagat
     8761 gcgacctccc aatccccagc aattctcatc gtgacggcac gtcgagcgcc gggtccgggg
     8821 gtccggccta caaccgccgc atatgagaat cgctgctcag tccgcagtaa tcctcttgac
     8881 cccggctcgg accttcttcc aacttgccgc agattgtccg gaccacggcg tcaatgcagg
     8941 ccagaagcgg tccggcgggc atctcatgtc aagagaccag attctgaagc tgaccgcgat
     9001 gtcttcgtgc gccggctgag cgtccaagct cggccaggag gccctgtcgc aggtcctgcg
     9061 gcagatcccg cgaactcgcg atgcgcgtgt gctgatcggc gccgatgcgg ccgacgatgc
     9121 cggggtgtgc cgcctcgaag ccggacgcgc cctcatccag acggtcgact tcttcacgcc
     9181 ggtggtggat gacccgtacg attatggcca gattgcggcg gccaacgccc tctcggacgt
     9241 gtttgcgatg ggcgggcgtc cgctcacggc gctcaacatt ctgtgcgtgc cagaggaaca
     9301 tcttcgtccc gaaactgtca ggaagatact cctcggtggc gcggccaagg ctcgggaagc
     9361 ccgttgccca atcatcggag gccacagcgt caagagtccg gaaccgatct acggcctggc
     9421 ggtgacgggg attgtcgacc cccgccgcct catcaccaac gcgtctgcgc ggcccaacga
     9481 ccatctgctg ctgaccaaac cgctgggcac cggcattgtg acgaccgcca tcaaggaagg
     9541 gctggccccg tccgctctgg cacaccgggc cgtgtcatcc atgcgacgcc tgaacagtgt
     9601 ggggccggac ctggcggagc gccggttggt gcgcgcgggg accgacatta ccggcttcgg
     9661 actgctcggg catctggcat cgctgtgccg ccaaagcgga gtgggcgcgc aaatcgatgt
     9721 cagcgccgtg cctgtgctgg gacggggggt ttgggacctg atcggacgag gctgcatccc
     9781 ggggggcacc cggcggaatc tcgggagcgc cgagtcgatc gccgactggt tgaacgtcac
     9841 cgaggagcag aagctgctcc tggccgatgc ccagaccagc ggcgggcttc tcctctgcgt
     9901 cgccgacaga cacctggcgg gtgtcctggc gcggcttcgg gcccatgaca cagccagcgc
     9961 ggcgcggatt ggccggatcg tccgctcgtc cggcaatcgg attctggtga agggatagag
    10021 atcggagcgg aaagaacaac ggcggcctcg acacgaggcc gccgttggct cgattgggag
    10081 agagtgaggc ggaggctcag aggcccttgg cgatcatgaa cttcacaagt tcagaaacgc
    10141 ggcagctata gccccactcg t
//
LOCUS       DAOQRO010000087        10161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5906_length_10161_cov_4.762517, whole genome shotgun sequence.
ACCESSION   DAOQRO010000087 DAOQRO010000000
VERSION     DAOQRO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_10161_cov_4.762517"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKK20_04855"
     CDS             <1..897
                     /locus_tag="PKK20_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99248.1"
                     /translation="EHSMMPQMAVGTDHVVYVAWNERGTPQSLEICAVTERGARIGPT
                     HTICRLRAPVSPIPLRRSNTTSNPSDWFKAFCLATLAVNPDPARSNHLYVAYADQSAD
                     QGDRADVFFVQSTDGGVHWTTKPIRVNLDTGLNDQWMPALAVRPDGAMLFVGWHDRRN
                     DPDNSLIEVCGRWGAIGADGVVSFFTNDFTITSEPFPPVFSGSLPEQTQPGHYDPVWA
                     PGGVNLHWWYPWYPEFDDWGQEVLTSDIYSHESGEHNQAYADAHYVYMVWSDNRSRSQ
                     ATRHPPRNQADIRLARLPWPQP"
     gene            complement(902..2200)
                     /gene="icd"
                     /locus_tag="PKK20_04860"
     CDS             complement(902..2200)
                     /gene="icd"
                     /locus_tag="PKK20_04860"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNU99249.1"
                     /translation="MTPPRGSSIGIRNGALTVPDDPILPFIRGDGTGPDIWAASQRVF
                     DAAVERAYGGKRKVAWFEVFAGQAAKDRFGDWLPEETIAAFQNFLVGIKGPLTTPVGG
                     GIRSLNVALRQQLDLYVCLRPVRYFRGVPSPVKHPEKVDMVIFRENTEDIYAGIEYPG
                     GSAEAGKMLEFLAREFPQAFEKIRFGTRAQADEFWRRAGAALPSDVCVGLGIKPVSRS
                     GSVRLVQSAIAHAIRFGRKNVTLVHKGNIMKYTEGAFRDWGYRAAKELFGAVEIDGGP
                     WCRIPEGRPGAGIVIKDAIADITLQQVLTRPEDFDVIATLNLNGDYLSDALAAQVGGI
                     GIAPGGNINFISGHALFEATHGTAPKYANQDKVNPGSMVLSGEMMFRYLGWTEAADLI
                     IRGLEGAIASKRVTYDFARLMEGATEIRCSQFGDNIIGHM"
     gene            complement(2215..4200)
                     /gene="dxs"
                     /locus_tag="PKK20_04865"
     CDS             complement(2215..4200)
                     /gene="dxs"
                     /locus_tag="PKK20_04865"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNU99250.1"
                     /translation="MSRYLDMVDSPDHVKKLTLDQLNLLAEEVRHEMITVLSKNGGHL
                     GPNLGVVELTIALHRVFSTPKDKFVWDVSHQCYAHKLLTGRKNRFNTIRTTGGLSGFA
                     MRAESEHDAYGAGHAGTALSAALGICAARDQRGSDEHVVCIFGDAALTNGMSFEALNN
                     ISHTTRRFIGILNDNEWSIAKNVGAIASYLNQLIRHPRYGRLQKDFERLLRRMPKGEL
                     AIKLGHKAEEALKGAVSGVALEPGTRQPGTEGRGGYGSSLIFEEMGLHYLGPIDGHNL
                     PLLIDSFTYAKECDRPVVLHVVTRKGQGYEAALKSPEKFHGVGPYDLVTGDPIAAAPG
                     TPQNYQDVFGLALVRFCQRDSTLVGITAAMPAGTGLKFLEQAMPQRYYDVGIAEGHAV
                     LFAAGMATQGFHPVVAIYSTFLQRSYDGIIHDIALQDLPVIFAMDRSGLAANDGPTHH
                     GLFDIAFLRCVPNIIAMAPKDEDELVDMLFTATHQNHPVFIRYPRGAGEGVAIKDQPR
                     LLTIGQAEITQHFSQSGGRKVALFALGNLQRLARDTAAGLKAVGCDVAIVNPRFIKPL
                     DEATTLLLGRASDAVVTFEDHVLAGGYGSSVIELYSNHGVTTPVVRIGWPDQFIEHAS
                     TADELREQYGLTAERAVAEVKARLQQRLPAAAVTALA"
     gene            complement(4229..4513)
                     /gene="xseB"
                     /locus_tag="PKK20_04870"
     CDS             complement(4229..4513)
                     /gene="xseB"
                     /locus_tag="PKK20_04870"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNU99251.1"
                     /translation="MSKAAKKPEASGDALSEMPFEQALQHLESVVEAMESDDLPLETL
                     LARYEEGVRLLEACQVKLAAAEVRISQLEQSASGTSALTPMTPEGGKDIQ"
     gene            4704..5468
                     /gene="infC"
                     /locus_tag="PKK20_04875"
     CDS             4704..5468
                     /gene="infC"
                     /locus_tag="PKK20_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNU99252.1"
                     /translation="MSRPFSSHRYPPSAGSFVRINGKIRAREVRVIGPDGQQVGVVSL
                     HEAINLARRSAMDLVEVAPNATPPVCRVVDYGKYRYEQAKKEKESKKHQHANRVKEIQ
                     LTATIDDNDFGVKLGHATGFLCEDMRVKVSLRFRGREMAHQEFGMQVMERFVKELAAY
                     GQAVEVPKLVGRNLHVMLTPLPRSKRAKNPAQTEPAQAPKTEARGTARAAPPDTDDRV
                     VDFPAPNGVTNESGRSGFTNNPFSQLSAQREEEAGA"
     gene            5475..7040
                     /locus_tag="PKK20_04880"
     CDS             5475..7040
                     /locus_tag="PKK20_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU99253.1"
                     /translation="MTDSVPGGPPSPEEFQRQLAELMRQTFSGGASGFTQPNPARGGS
                     GGSDSASGVEFAFNQKPREVKAYLDRFVIRQEEAKKVLSVALCDHYHHVRLALEGQDQ
                     PNYAKQNVILIGPTGVGKTYLVRSIADLIGVPFVKGDATKFSETGYVGADVEDLVREL
                     FRRANGDVKRAQYGIIYIDEIDKIAASVNHAGRDVSGRGVQTNLLKLMEETDVAARSP
                     NDIAGQLQAMMEMTQRGRKPVDVINTKHILFIVSGAFPGFDKVVRKRLREATIGFAAR
                     TASVEESNEVMACAQTRDFIEFGFEPEFIGRLPVRVYCHPLSVDDLFTILKTSEGSII
                     RQYEQTFAAYGIEVLFRADGLRRIAELANEEQTGARGLMTVCERVFREFKYELPSTSV
                     KRFVVTRDLVDQPMPALAKLLAEHAQDERAVLRQLVNDFAERFTKDHGIQIAFTPAAA
                     DRLVALATETGRPVRDLCAERFRDYQFGLKLITQNTGQREFQLDLDAVESPDETLSKW
                     VVTSYRPATSEHA"
     gene            6995..7429
                     /locus_tag="PKK20_04885"
     CDS             6995..7429
                     /locus_tag="PKK20_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99254.1"
                     /translation="MGRHQLSPRDIRTRLTRISHHLLETWHRNPHARWTTRPEDVAEL
                     AQIQLRLLRHAVSALKPGGRLIYAVCTLTRAETTAIADALTHERLELDRLPLPSVLPS
                     AQCTPACLWLDGGARGGERHVGRGMAAARFDHHACQRGGPPL"
     gene            7359..8792
                     /locus_tag="PKK20_04890"
     CDS             7359..8792
                     /locus_tag="PKK20_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNU99255.1"
                     /translation="MLVAAWRRRASITTLANEGGRLFNGRVVQQQTLREPVRLSGIGL
                     HSGNRVTMGLLPAPPHSGIRFRRIDLEGQPEIEAHIDNVVDTTRSTSLGKGSVRVHTV
                     EHILAALTGCGVDNAVVELDAGEPPVTDGSAREYSRAIEAAGIVPQGEAREIYSVNEP
                     LEISSGDTFMAVFPHDRLKVTCTSSDRQGRHTQCRSIELSAETWAREIAPARTFCFFE
                     EIEHLIKNGLIKGGGLENAVVIRDDAVLTTEPLRFADEFVRHKILDVVGDLSLVGRPV
                     AGHVVAVRPSHAANCGLARLISAQMRKVLAATQAFAPPPHPRDAAPATGGKPLAPLTD
                     GSTLDADELLKILPHRYPFLMVDRVTRIEGNTITGVKNVTVNEPFFAGHFPGHPIMPG
                     VLQLEAIAQVAGILMLRQAEYSRKLAYFMSAEVVKWRRPVRPGDTLIIQVELTKWRGK
                     IGRAKGVCLVGEDVVSEAEVTFMLVDA"
     gene            8813..9844
                     /locus_tag="PKK20_04895"
     CDS             8813..9844
                     /locus_tag="PKK20_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99256.1"
                     /translation="MQTDNPPHISKKPRPRIKRPPVLFNRTQKIVERIERKVGRCFLT
                     YWNSNNGSVCQNDVIAIYGLLRRLGPQPQITLFVKSDGGSGQASLMMVNLLRQFTKRL
                     VVLVPLECQSAATMLALGADAIEMGPLAHLSAVDTSLAHALSPLDRDNERVSVSQDEL
                     MRVIRLWQKEVKADAVNPYQAVFPYIHPLVIGAVHRISSLSVKLCMEILSYHMKDRKK
                     AAAISQCLNSSYPSHSYPITLREAQRIGLAARPLDNALNDLLLELNELYSEMGQRAVT
                     DYDALNNHDSQICNIIEGHGLQAFYQSDRDWHYRKEERRWVTLNDHSAWRKVEKVRGR
                     TVSSVFHIS"
     gene            9857..>10161
                     /locus_tag="PKK20_04900"
     CDS             9857..>10161
                     /locus_tag="PKK20_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNU99257.1"
                     /translation="MTHLKGRTALLTGADGGMGGHLATALAAEGVNLLLVGFGEHGLA
                     ALVDALTARGVCAKSIHADLRDASQRRDVVATARREFGDIDLLVNNAGVEYSCPYHEL
                     "
BASE COUNT         1883 a         3272 c         3200 g         1806 t
ORIGIN      
        1 gagcactcga tgatgccgca gatggcggtg gggaccgacc atgtcgtcta tgtggcgtgg
       61 aacgaacggg gcacgccgca gtcactcgag atctgtgccg tgacggaacg cggcgccagg
      121 atcggcccca cacacaccat ttgcaggctc agggcgccgg tctcgcccat cccgcttcga
      181 aggagcaata cgacttcgaa tccgagcgac tggttcaagg ccttttgcct ggcgacgctg
      241 gcggtgaatc ctgatccggc ccgctcgaac cacctctacg tcgcctacgc cgaccagagc
      301 gccgaccagg gcgatcgcgc cgacgtcttt tttgtgcagt cgaccgatgg cggcgttcat
      361 tggaccacga aaccgatccg tgtgaacctc gacaccggcc tgaatgatca gtggatgccc
      421 gctctcgccg ttcggcccga cggcgcaatg ctgttcgtcg gatggcacga ccgccggaac
      481 gatccggaca actcgctcat cgaggtgtgc ggacgctggg gcgcgattgg agccgatggc
      541 gtggtttcat tcttcacgaa cgacttcacg atcacatccg agccgtttcc accggtattc
      601 tccggctcac tgcccgagca gactcagccg ggccattacg acccggtctg ggcgccaggc
      661 ggggtgaacc tccactggtg gtacccgtgg tatcctgaat tcgacgattg gggtcaggag
      721 gttctgactt cggacatcta ctcgcacgag agcggcgagc acaatcaggc ttacgccgac
      781 gcgcactatg tctatatggt gtggtccgac aaccgcagcc gctcccaggc cacacggcat
      841 cccccaagaa accaagccga tattcgtctg gcgcgtctgc cctggccgca gccctgatgc
      901 ttcacatgtg cccgatgatg ttgtctccga actgcgagca ccggatttcc gtcgcccctt
      961 ccatgagccg cgcgaaatcg taggtcacac gcttgctggc aatggcccct tcgagccctc
     1021 ggatgatcag gtccgccgct tcagtccagc ccagataccg gaacatcatc tcgccgctga
     1081 gaaccatgct gcccggattg accttgtcct ggttggcgta cttgggcgcg gtgccgtgcg
     1141 ttgcctcgaa gagggcgtgg ccgctgatga agttgatatt gccgccgggc gcgatgccga
     1201 tgccgcccac ttgggctgcg agcgcgtcgc tcaggtaatc gccgttcagg ttcagcgtgg
     1261 cgatgacatc gaaatcctcg ggccgggtca gcacttgctg gagcgtgatg tcggcgatgg
     1321 cgtccttgat gacgatgccc gccccgggcc ggccctcggg gatgcggcac cagggaccgc
     1381 cgtcgatctc gacggcgccg aacaactcct tggcggcgcg gtagccccag tcgcggaacg
     1441 cgccctcggt gtacttcatg atgttgccct tgtgaacaag ggtgacattc ttgcgcccga
     1501 atcgaatggc atgggcgatc gcgctctgga ccaggcgaac gctgccggac cggctgaccg
     1561 gcttgatccc gaggccaacg cagacgtccg acgggagcgc ggcgccggca cgccgccaga
     1621 actcatcggc ttgggcgcgg gttccgaacc ggatcttctc gaaggcctga ggaaactccc
     1681 gggcgaggaa ctcgagcatc ttgccggctt cggccgagcc gcccgggtac tcgatgcccg
     1741 cgtaaatatc ctccgtgttc tcccggaaaa tcaccatgtc gaccttttcc ggatgtttca
     1801 cgggagaggg cacgccgcgg aaatagcgca ccggacgcag acagacgtaa agatccagtt
     1861 gttggcgcag agcgacgttg agcgatcgaa tgcccccgcc gacaggcgtg gtcagagggc
     1921 ccttgatgcc caccagaaag ttctggaacg cggcgatggt ctcctccggc agccaatcgc
     1981 cgaacctgtc cttggccgcc tggccggcga agacctcgaa ccacgcgact ttgcgcttgc
     2041 cgccgtaggc ccgctcgacc gcggcgtcga agacccgctg gctggccgcc cagatgtccg
     2101 ggcccgttcc atcgccgcgt atgaacggca ggatcgggtc gtcggggaca gtcaaggcgc
     2161 cgtttcgtat gccgatactc gaaccgcgag gtggagtcat aattgaagag ggggtcaggc
     2221 cagagcggtc accgcggcag cgggcagccg ctgctgcaac ctggctttca cctcggcaac
     2281 ggcgcgctcg gcggtcagcc cgtactgctc gcgaagctcg tccgccgtcg aggcatgctc
     2341 gataaactgg tccggccagc cgatccggac gaccggagtc gtgacaccgt gattgctgta
     2401 caactcgatc acgctgctgc cgtatccgcc ggccagcaca tgatcctcga acgtgaccac
     2461 ggcatcggac gcccggccca acaacagcgt ggtcgcctca tcaagtggct tgatgaaacg
     2521 cggattgacg atggcgacgt cgcacccgac ggccttgaga cccgcagccg tgtcgcgtgc
     2581 cagacgctgc agattcccaa gggcgaacag ggccactttc cgcccgccgg actggctgaa
     2641 gtgctgggtg atctcggcct ggccgatcgt gagcaaacgg ggctggtcct tgatggccac
     2701 gccttcgccg gcgccgcgcg ggtagcggat gaagaccgga tggttctggt gggtggcggt
     2761 gaagagcatg tcgaccagtt cgtcttcgtc tttgggcgcc atggcgatga tgttgggcac
     2821 acagcgcagg aaggcgatgt cgaacaggcc gtggtgggtt gggccgtcat tggccgcgag
     2881 gccggaacgg tccatggcaa aaatcacggg cagatcctgg agggcgatat cgtggatgat
     2941 gccatcgtag gaccgctgga ggaaagtcga gtaaatcgcg acgacggggt gaaagccctg
     3001 tgtggccatg ccggcggcga acaacacggc gtggccctca gcaatcccaa cgtcgtagta
     3061 gcgctggggc atggcctgtt cgaggaactt caagccggtc ccggcgggca tcgccgcggt
     3121 gatgccgacc agggtgctat cgcgctggca gaaccggacg agcgccaggc cgaacacgtc
     3181 ctggtagttc tggggtgtgc ccggcgcggc ggcgatcgga tcgccagtga ccaggtcgta
     3241 gggaccgacc ccgtggaact tctccggcga cttgagcgcg gcctcgtacc cctgtccctt
     3301 gcgcgtgacc acgtggagga cgaccggtcg atcgcactcc ttggcgtagg tgaaggaatc
     3361 gatcaggagc ggcagattat gtccgtcgat gggcccgagg taatggaggc ccatttcctc
     3421 aaagatcagg ctgctgccgt agcctccacg cccctcggtg ccaggctggc gggtgcccgg
     3481 ttcgagggcg acacccgaaa ccgctccctt gagcgcttcc tcggccttgt ggccgagctt
     3541 gatggccagc tcccctttgg gcatgcgccg taaaaggcgc tcgaaatcct tctgcagccg
     3601 accgtaacgc ggatgccgga tcaactggtt gaggtaagag gcgatggcgc cgacgttttt
     3661 ggcgatgctc cattcgttgt cgttgaggat gccgataaac cggcgtgttg tgtggctgat
     3721 attgttcagc gcctcgaagg acatcccgtt ggtaagcgcc gcatcgccga agatgcagac
     3781 cacgtgctcg tcactgcccc gctgatcccg cgccgcgcag atgcccaggg cggccgacag
     3841 cgcagtcccg gcgtggccgg cgccgtaggc gtcatgctcg ctctcggccc gcatggcgaa
     3901 cccgctcaac ccgccggtcg tccgaatggt attgaaccgg ttcttccgcc cggtgagcag
     3961 cttgtgggca tagcattggt ggctgacgtc ccagacgaac ttgtccttgg gtgtcgagaa
     4021 gacccggtgc agcgcgatgg tcaattccac cacgccgagg ttgggaccca aatgtccgcc
     4081 gttcttcgag agcacggtga tcatctcgtg gcggacctcc tcggccagca ggttgagctg
     4141 atcgagcgtc agtttcttca cgtggtccgg actgtcgacc atgtccaggt agcgactcat
     4201 ggtgtttgcg tgtgtgtggc tccgcccgtc actggatgtc cttgccgccc tccggagtca
     4261 tcggggtcag cgccgacgta ccggacgcgc tttgttcgag ttggctgatt ctgacctctg
     4321 cggcggcaag cttgacctga caggcttcga gcagtctaac gccctcctca taccgcgcga
     4381 ggagggtctc cagggggaga tcgtccgact ccatcgcttc cacgacggat tcgaggtgct
     4441 gcagggcttg ctcgaaaggc atctcgctca gggcatcgcc cgacgcctcc ggtttcttgg
     4501 ccgctttcga catcaggcgg gcaggatagg ggaagtcggg cgggccgtcc agcgtgttgc
     4561 ttggccggcc gatccgcgaa cgacgatttt gacttgcgtc ggaagggatg atggtgaata
     4621 agtaacagtg ttctcgctgt ccaattgacg gcatgcaagt tgagtcgaca acgcatgaat
     4681 gagcttcaac cggggagggc ttcttgagcc gtcccttctc ctcacatcgt taccctccat
     4741 ctgcaggttc cttcgtccgg atcaatggaa agatacgtgc ccgcgaagtt cgtgtcatcg
     4801 gtcctgacgg gcagcaagtg ggtgtcgttt ccctgcacga agcgatcaat ctcgcccgcc
     4861 gcagcgccat ggaccttgtc gaggtcgccc ccaacgcgac gccgccggtc tgccgggtgg
     4921 tcgactacgg caagtaccga tacgagcaag ccaagaaaga gaaggaatcc aaaaagcacc
     4981 agcacgccaa tcgcgtgaag gagatccagc ttaccgccac gatcgacgac aacgatttcg
     5041 gcgtgaagct ggggcatgcg acggggtttc tgtgcgagga catgcgggtg aaggtcagct
     5101 tgcgattccg gggccgcgag atggcccatc aggaatttgg catgcaggtc atggagcgct
     5161 tcgtcaagga actggccgct tacgggcagg cggtcgaggt gcccaaactc gtcgggcgca
     5221 acctgcacgt catgctgact cctctgccga gaagcaaacg cgccaagaat ccggcacaga
     5281 ccgagcctgc ccaagccccg aagaccgaag cccgcggcac ggcaagggca gccccgcccg
     5341 acacagacga ccgtgtcgtc gatttccccg ctccgaatgg ggtgacgaac gagagcgggc
     5401 gctcgggttt caccaacaat ccgttctccc aattgagcgc ccagcgcgag gaggaggccg
     5461 gcgcctgaca gcccatgaca gattcagtgc caggcggccc gccgtcgcct gaggagttcc
     5521 agcgccaact ggcggagttg atgcggcaga ctttttcggg gggggcgagc ggtttcacac
     5581 aacccaatcc ggcccgcggg gggagcggtg ggagtgattc cgcgagcggg gtcgagttcg
     5641 cattcaatca gaagccgcgg gaggtcaagg cctatctgga ccggtttgtc atccggcagg
     5701 aggaagcgaa gaaggtgctg agtgtggcct tgtgcgatca ctatcaccat gtgcgcctgg
     5761 ccctcgaggg acaggaccaa cccaactacg ccaaacagaa tgtcattctc atcgggccta
     5821 cgggggtcgg caaaacctac ctcgtccgga gcatcgccga tctcatcgga gtgcccttcg
     5881 tcaagggcga cgccaccaag ttctccgaga ccgggtatgt gggggccgac gtcgaggatc
     5941 tggtgcgcga gttgttccgg cgcgccaacg gcgacgtgaa gcgggcccag tacggcatca
     6001 tctatatcga cgagatcgac aagatcgcgg cgtccgtgaa ccatgccggg cgcgacgtga
     6061 gcgggcgcgg cgttcagacc aacctgctca aactcatgga ggagacggac gttgcggcgc
     6121 gatcgcccaa cgacattgcc ggccaattgc aggcgatgat ggagatgacg cagcgaggca
     6181 gaaaacccgt ggacgtcatc aacaccaagc atatcctctt catcgtcagc ggcgctttcc
     6241 ccggcttcga caaagtggtc cgcaaacgcc tgcgcgaggc gaccatcggt ttcgcggccc
     6301 gcaccgcgtc ggtcgaggag agcaacgagg tgatggcgtg cgcccaaacg cgcgatttca
     6361 tcgagttcgg cttcgaaccc gagttcatcg ggcgcctgcc ggtgcgcgtc tactgccatc
     6421 cgctctccgt cgacgacctc ttcaccatcc tgaaaacatc cgagggcagc atcatccgcc
     6481 agtacgaaca gaccttcgct gcctatggca tcgaggtgct gtttcgcgct gacgggctgc
     6541 gacgcattgc ggaactggcg aacgaggaac agaccggcgc gcgcggcttg atgacggtgt
     6601 gcgagcgcgt ttttcgcgag ttcaagtacg agttgccatc cacctccgtc aaacggttcg
     6661 tcgtcacccg ggacctggtc gaccaaccga tgccggccct ggcgaaactg ctggccgagc
     6721 acgcccagga cgaacgggcc gtgctgcggc agttggtgaa tgacttcgcc gaacggttca
     6781 ccaaggatca tgggattcag atcgccttca cgccggccgc cgccgaccgc ctcgtcgcgc
     6841 tcgccacgga aacaggccgc ccggtgcgcg atctctgcgc cgagcgcttc cgcgattacc
     6901 agttcgggct caagttgatc actcaaaaca ccggccagcg cgaattccag ctcgacctcg
     6961 atgcggtgga aagccccgac gagaccctca gcaagtgggt cgtcaccagc tatcgccccg
     7021 cgacatccga acacgcctga cgcggatttc tcaccacctt ctggagacgt ggcatcgcaa
     7081 cccgcacgcc cgctggacga cgcgtccgga agatgtggcg gagcttgcgc agattcaact
     7141 gcgtttgctg cgtcacgccg tttcggctct gaaacccgga ggccgcctga tctacgcggt
     7201 ctgcacgttg acacgagccg aaaccacggc gatcgcggat gcgctcacgc acgagcgtct
     7261 ggagctcgac cgtctgcctc tgccgtccgt cctgccgtcg gcgcagtgca cgccggcctg
     7321 cctttggctt gacgggggcg ccagaggggg ggaacggcat gttggtcgcg gcatggcggc
     7381 ggcgcgcttc gatcaccacg cttgccaacg aggggggccg cctctttaat ggccgcgtgg
     7441 tccaacaaca aactctgcgc gagccggtgc gactgagcgg catcggcttg catagcggca
     7501 accgggtcac catggggctg ctgcccgccc cgccccactc cgggatccgc tttcgccgca
     7561 tcgacctcga aggccagccc gaaatcgaag cccacatcga caacgtggtc gataccaccc
     7621 gctcgacctc tctgggcaag gggagtgtcc gggtccacac cgtcgagcac atcctggcgg
     7681 ccctgaccgg atgcggggtc gacaatgcgg tggtcgaact cgatgctggc gaaccgccgg
     7741 tgaccgacgg gagcgcgcgg gagtactcgc gcgcgatcga ggcggctgga attgttcccc
     7801 agggcgaggc gcgtgagatc tactcggtca acgagcccct cgagatatcc agcggagata
     7861 cgttcatggc ggtgtttccc catgaccgcc tcaaggtgac atgcacgagt tcggatcggc
     7921 aaggccgcca tacccagtgc cgttcgatcg agctctcggc ggagacgtgg gcccgcgaga
     7981 tcgcgccggc gcggaccttc tgcttcttcg aagagatcga gcacctgatc aagaacggcc
     8041 tgatcaaggg cggcggcctg gaaaacgccg tggtgatccg cgatgacgcc gtcctgacaa
     8101 ccgaacccct gcggtttgcc gatgaattcg tgaggcacaa aatcctggac gtcgtgggtg
     8161 acctcagcct ggtgggccgg ccggtggcag gccacgtggt ggccgtccgc ccgagccacg
     8221 ccgccaattg tggactggcc cgcctcatca gcgctcagat gcgcaaggtt ctggcggcca
     8281 cccaggcctt cgccccaccg ccgcatccgc gggatgcggc cccggcaacg ggcggcaaac
     8341 ctctggcgcc gctcaccgat ggctctacgc tcgatgccga cgagttgctc aagatcctcc
     8401 cccaccgcta tccctttctc atggtggatc gggtgacacg gatcgagggc aacacgatca
     8461 ccggcgtgaa gaacgtgacg gtcaacgaac cgttcttcgc cgggcatttc cccggccacc
     8521 cgataatgcc gggcgtgctc caactcgaag cgatcgccca ggtggcagga atcctgatgc
     8581 tgcgtcaggc cgagtattcc cgcaaactcg cctacttcat gtcggccgag gtggtcaagt
     8641 ggcgccgccc ggtccggccc ggcgacacct tgatcatcca ggtcgaactc accaagtggc
     8701 gcgggaagat cgggcgggca aaaggggtgt gtttggtggg agaggacgtc gtcagcgagg
     8761 cggaggtgac gttcatgctc gtcgatgcct gacacttcgg atacatcact tcatgcagac
     8821 cgacaaccca ccccatatct cgaagaagcc gcgcccccgc atcaagcgtc cgcccgtgct
     8881 cttcaatcgc acccagaaga tcgtcgagcg gatcgagcgt aaggtcggac ggtgcttcct
     8941 gacctactgg aactcgaaca acgggtcggt ctgccagaac gacgtcatcg cgatctacgg
     9001 cttgctgcgc cgactggggc cccagccgca aatcaccctc ttcgtcaagt ccgacggcgg
     9061 gtccggccag gcctccctga tgatggtgaa tctgctgcgc cagttcacca aacgcctggt
     9121 cgtgcttgtc ccgctcgaat gccagtcggc cgcaaccatg ctcgccctcg gcgccgacgc
     9181 catcgagatg ggtcctctgg cccacctctc ggccgtcgac acctcgctcg cccacgccct
     9241 ctccccgctc gaccgggaca acgaacgcgt cagtgtcagc caggacgaac tcatgcgcgt
     9301 catccgcctc tggcaaaagg aggtcaaggc cgacgccgtg aatccgtacc aggcggtctt
     9361 cccctacatc caccccctgg tcatcggggc agtccaccgg atcagctcgc tctcggtcaa
     9421 actctgcatg gagattctct cctatcacat gaaggatcgg aagaaggccg cggcgatcag
     9481 ccaatgcctc aactcgagct acccatccca cagctacccc atcaccctgc gcgaggccca
     9541 gcggatcgga ctggccgccc gcccactcga caacgcgctc aacgacctgt tgctcgaact
     9601 caacgaactc tactcagaga tgggccagcg ggccgtcacc gattacgatg ccttgaacaa
     9661 tcatgacagc caaatctgca acatcatcga agggcacgga ctccaggctt tctaccaatc
     9721 cgatcgcgac tggcattacc gcaaggaaga acgccgctgg gtcaccctca acgaccacag
     9781 cgcctggcgc aaagtggaga aagtacgtgg acgcaccgtg tcttcagtgt tccacatcag
     9841 ttgacgggag tgttccatga cccatctgaa aggtagaacg gctctcttga ccggggccga
     9901 tggcggcatg ggaggccatc tcgccaccgc tctggcagcc gaaggcgtca atctcctctt
     9961 ggtcgggttc ggggagcacg gtttggccgc tctcgtcgat gccctcacgg cccgaggcgt
    10021 gtgcgcgaaa tccatccacg ccgatcttcg ggatgcgtcg caacgccggg atgtcgtagc
    10081 taccgcgcgg cgggagttcg gcgatatcga cctgctcgtc aacaacgccg gtgtcgagta
    10141 ctcgtgcccc tatcacgaac t
//
LOCUS       DAOQRO010000088        10144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_5922_length_10144_cov_5.840817, whole genome shotgun sequence.
ACCESSION   DAOQRO010000088 DAOQRO010000000
VERSION     DAOQRO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5922_length_10144_cov_5.840817"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..2665)
                     /locus_tag="PKK20_04905"
     CDS             complement(146..2665)
                     /locus_tag="PKK20_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99258.1"
                     /translation="MKTPLRAIQQRSTALGASAWLALIATGLVLGANPARAASAKLNE
                     ALSAYDSAITGDSTAALAKLTSAVTLDGTAGEPFNFGATSGDTTMEFILEGDPVATGN
                     SGYLAVGENMTSNLRYDQYPDRRQMAFTQLGVADYLFDPGVPSPTAPTHITYVWNTLD
                     LSMTLYVDGVMAGTVWSVSDAFAMPTGAGFLGANAAGGEQMVGTIYRVTVYPGVLPDD
                     KIKSHADAFVNARGPLVGISSFTATPTQVLVQGSTVLSWETQNATAHFINGVSVTGTS
                     VTLSPAVTTTYTLVASNDVSVASAQVTVQVEPPLALYDEVIAADTAAGLTPISMLADT
                     VDLDGTAGVFFDFGYTWGSTSMEFILEGDPLASGGSAFLAVGENSGSSLRYEQWQDTR
                     EMGFTQGGIADYRFSPSVSSPTIATHVAYIWDADEFSMTAYVNGVAKGKVTDVTDAFA
                     MPTGYGWLGAAADGSQLMVGRIFRLVVYGEALPEATVKRHAEAFTSVLRAPIITSFTA
                     DPAEIVGQGSSTLSWDVQYATGVYVNNAPVAGTSLTVSPAATTTYTLLASNAVSTVSA
                     KLTIYVTPLLDAYDAVIAADAASGLTPVSSLASVVTLTGSGGVPFDFGGTFGDVTMEF
                     ILEGDPAAGADAYLAVGEVSTSSLRYAQWQNTRQMGFTQSGVSDYMFTPAVPSPTAAT
                     HVAYAWNAAEFVMKVYINGALGGISTGVSDAFVMPSGPGYLGATAGGAEAMTGRIYRL
                     TVYNGLLPEATLLKHAAAFTGGGDGPSLSIDLQAGLAGITLEGVPGIHYRVEYRDSLA
                     AADTWQLLHDIPALGGTSIRVVDPTSAVGRTQRFYRAVTVR"
     gene            complement(2849..7102)
                     /locus_tag="PKK20_04910"
     CDS             complement(2849..7102)
                     /locus_tag="PKK20_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNU99259.1"
                     /translation="MRSFRSATSSVAAFLVACTGLSGLRAADLFQPDPAALTRVAAQL
                     PSEPGAFGPACTDREAWDRIASRPEMQQHVRHAEEEMRTPLPDLPDALFLDYSKTGNR
                     RRCETVLFARRARASSLALAECIENRGRFLEPLEATLQSIARERTWVMPAHDGGLDNF
                     HGRQVTIDLAVAMFAWELSTIDRVLAAKLSPSLRTTIRQELERRVFAPYRRMTAGEQD
                     QFWLLHVHNWNAVCLAGVTGAALASLDDPKERAWFVLAAEKYSANFLEGFTPDGYCSE
                     GVGYWNYGFGNYVALAEAVRRATGGGIDLLARPKAIKPALYGLQIEIADGVCPAFADC
                     DVGARPGSRLVGLLRRRFGLADEPTALAPPTRGSFQAALMDLFPSPAPPIPLSEPIPT
                     ASAPRTWFPDAGVLICRPGSSAGTRLAVAIKGGHNAEHHNHNDVGTFIAVVGGVAVLP
                     DIGSEVYTQRTFSARRYESRALNSFGHPVPLIAGKMQSPGRQAAARLLALEQSDEGTD
                     KVVFDLAAAYDVPSLSKLTRTCTYSRTGSGSLTVIDEFAFAQAETFETALLTFGRCEQ
                     LSSRVLQITDGAEAVRVEISVPDNIPLDIRAEPIREDLVARRTATRIGLRLKEAVRDG
                     KVTVVITPSSVGAHAFLEKLSTLPGTASPTLEVAAKALADGQTQKARDGFDAIARDAS
                     APPFVRGLAWCGLADLALAGESSDDIEGLRKRLAADATVPTLYRDAALRRLTEANRSR
                     QAEDDSADEAESDRVRLPDVSRSTALFHMAPNGNDSNDGTEGNPFLTLHRARDAVRTL
                     KQARGGPLPSGGVTVVVHGGMYAVSETFALNTEDSGRPDAPVVYRAGEGETPVFSGGI
                     RIANWTPIEDLQVRERLAASARDRVLAADLGALGVVDYGDPTDLRRRPELFVNGVPQT
                     LARWPDEGFVKTGELLGQDTFKVWNSIAGCRDGRFRYIEERPSTWADEPDVRLYGYWF
                     WDWYEECQKVERIDPSEHSYTLAQPYSQYGYRSGQRYYALNVLRELDQPGEWYLDRQR
                     GKVFWLPPENVDVAKAEVVLSVLDRPFIALDNANHVLLLGLTVQDGRANGIEVRGGTN
                     CLVAGCTVRRLGGDGVVIEGGQRHGLFGCLLETLGCGGARVAGGNRQTLAAGRHFVEN
                     CTVSNISRLKRTYTPAVHLDGCGQRVAYNLFERMPSSAMRIEGNDHLVELNVIRDVVQ
                     ESDDQGGIDMFGNPLYRGTVIRWNRWSDIRGGTHNGAAGVRLDDMISGTVVQGNVFER
                     CGAVAFGGVQIHGGKDNWVDGNVFIDCFAGISFSRWGEKRWLEAIAKFIPQAADPAHV
                     ARYPELARLESAPDVNIVTRNLFVRCGQEFLRDGNTQQTALNASSDRPLDLEAVSSAG
                     AAAQAPAVRALLFEPIPLSCMGPYEHPWRAQHPGAYAAR"
     gene            7272..7526
                     /locus_tag="PKK20_04915"
     CDS             7272..7526
                     /locus_tag="PKK20_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99260.1"
                     /translation="MQFETFSLTNDTLGAASPAAATGLAAADAESGSLNLAWTPGNGT
                     AGSVVIMRPSAPITRQPIDGVAYNGNAVFDDEKKELIYGT"
     gene            complement(7514..8479)
                     /locus_tag="PKK20_04920"
     CDS             complement(7514..8479)
                     /locus_tag="PKK20_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNU99261.1"
                     /translation="MAPWKRILVPVDFSLESMKTLSYASKLAQSLSACLDIVHVLTTR
                     PRGARVGAVPLLAGLDREDLAEAARAKLLAVIKRKVHWQVPWNVWIRDGQPHREILEA
                     AARYDVDALVLHTRSKPSLEGFVDQGVTRRLVFGASCPVLAIPPQVLWQSGESLPLVS
                     PDHWHTILIPTEMSRVGENALARGLALARWFNARVTAFNVLRTGRLYSHYRPTGARNR
                     RFVQTKSRFRTWVHRHAPPDVHLDMCVGVGSPASTFLRAARHMRAHLIVMGTQQFSGW
                     RRLRALRTISRILADTPCPILCVPQPPGGLQMPAAAVSTTRDQVP"
     gene            complement(8568..8729)
                     /locus_tag="PKK20_04925"
     CDS             complement(8568..8729)
                     /locus_tag="PKK20_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99262.1"
                     /translation="MALTFKHHMPKPEPGLAESVRAQLEGRDAVLTVIAIKPVQITPN
                     QEPHRHGPD"
     gene            complement(9125..9628)
                     /locus_tag="PKK20_04930"
     CDS             complement(9125..9628)
                     /locus_tag="PKK20_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99263.1"
                     /translation="MNTAELIALIAVACLLATEIILRLFVAPQNRDRLVKDLLDIMHE
                     PRLESDTTSGLVPTLLPSESLGEGLLRHFAQSSLSCRILEVLARNGNEMHMTDITAAV
                     NAQLIEKSRQPLPEVAVRKVTTILMEANFVRLQHGLLSLTFLGQQLHSVLGERRRAAR
                     APALTAL"
BASE COUNT         1808 a         3243 c         3352 g         1741 t
ORIGIN      
        1 acgacggcgg ctacggaggc gtagccgtcc cgtagccgcg gacgggagtc cgcgctgact
       61 cgctccggcg aatacaaacg acagcgggga gggagtggcg gcaacgccac ccccttaccc
      121 atcgcgatct gaacgcgcgc ggggactacc ggactgtcac ggcgcggtaa aagcgctgtg
      181 tgcgaccgac cgcggaggtg ggatcgacca cccggatgct tgtgccgccc agcgccggga
      241 tgtcgtggag cagttgccat gtgtccgcgg ccgcgagcga atcgcgatac tcgacgcggt
      301 aatggatgcc gggcacgcct tccagcgtga tgccggccaa gcctgcctgg aggtcgatag
      361 acagcgatgg accgtctccg ccgcccgtga acgccgcggc gtgcttcagg agcgtggcct
      421 ccggcagcag cccgttgtac acggtcagtc ggtaaatccg cccggtcatc gcttcggccc
      481 cgcccgccgt ggcacccagg taacccggtc ccgagggcat gacaaaggcg tcgctgacac
      541 cggtgctgat gcctcccagc gcgccgttga tgtagacctt catgacgaac tcagcggcat
      601 tccatgcgta tgcgacgtgc gtggcggccg tgggcgaagg cacggctgga gtgaacatgt
      661 aatcactcac tccgctctgg gtgaacccca tctggcgggt gttttgccat tgcgcatagc
      721 gcaggctgct ggtgctcact tcgccgacag ccaggtaggc gtccgcgccg gctgcggggt
      781 cgccttcgag aatgaactcc attgtcacat ccccgaacgt gccgccgaaa tcaaacggca
      841 caccgcccga cccggtgagc gtgaccacgc tggccagact ggacacggga gtcaaaccac
      901 tggcggcatc agccgcgatg acggcgtcgt aggcatcgag caggggcgtc acatagatgg
      961 tgagcttggc gctgaccgtg gacacggcgt tggatgccag cagggtgtag gtggtggtgg
     1021 cggcgggtga cacagtcaag ctcgtgccgg cgacgggggc gttgttcacg tacaccccgg
     1081 tggcatactg gacatcccag ctcaacgtcg aggagccttg ccccacgatt tcggcggggt
     1141 cggccgtgaa ggaggtgatg atgggggcgc gcagcacgct ggtgaaggcc tcggcgtgcc
     1201 gcttgacggt cgcctcgggc agcgcttccc cgtaaaccac caagcggaag atccggccaa
     1261 ccatgagctg cgagccgtcg gcggcagcgc caagccagcc gtatccggtc ggcatcgcaa
     1321 acgcgtcggt tacgtccgtg accttgccct tagccacacc gttgacatag gccgtcatcg
     1381 agaattcgtc cgcgtcccag atataggcca cgtgggtggc gatggtcggc gaagacacgg
     1441 aaggggagaa ccggtagtcg gcgatgccgc cttgggtgaa gcccatttcc cgcgtgtcct
     1501 gccactgttc gtagcgcagg ctgctgcccg agttctctcc caccgccaga aaagccgagc
     1561 cgccgctggc cagcggatcg ccctcgagaa tgaactccat cgaggtgctg ccccaggtgt
     1621 acccgaaatc gaaaaacact cccgccgtgc cgtccaggtc gaccgtgtca gccaacatgc
     1681 tgatgggagt caggccggca gcggtgtcgg ccgcaatgac ctcgtcataa agagccagcg
     1741 gcggttccac ctggactgtg acctgcgcgc tggccacaga gacatcattg gacgcgacca
     1801 gcgtgtacgt ggtggtgacg gcgggcgaca gggtgacgct cgttccggtc acgctcacgc
     1861 cgttgatgaa atgcgcggtg gcgttctggg tttcccagct cagcacggtc gagccctgga
     1921 ccaggacctg agtgggcgtg gccgtgaagg aagaaatgcc gaccagcggg ccgcgcgcat
     1981 tcacgaacgc atcggcgtgg ctcttgattt tgtcgtcggg gagcacaccc gggtacacgg
     2041 tgacgcgata gatggtgccg accatctgct cgccgccggc ggcattcgct ccgaggaagc
     2101 cggccccggt gggcatggcg aaggcgtcgc tgacgctcca aacggtgccc gccatgacgc
     2161 catcgacata caaggtcatg gagaggtcca acgtgttcca gacatacgtg atgtgcgtgg
     2221 gagcggtagg tgaaggcaca ccgggatcga acaggtagtc cgctacgccg agctgggtaa
     2281 acgccatctg gcgtctgtcc gggtactgat catagcggag gttgctggtc atgttctcac
     2341 ccacggccag ataacccgag ttgccggtgg cgaccggatc gccttcgagg atgaactcca
     2401 tcgtggtgtc gcccgaggtc gctccgaagt tgaacggctc gccggctgtc ccgtcgaggg
     2461 tgacggccga ggtgagcttc gccagggctg cggtgctgtc gcccgtgatg gcgctgtcat
     2521 aggcgctgag ggcctcattc agtttggccg aggctgcgcg tgccggattg gcgccgagaa
     2581 cgagtccggt cgcgatgaga gcgagccagg cgcttgcgcc aagggcggtg ctgcgctgtt
     2641 gaattgctct gagaggtgtt ttcattggaa ggactggagt cggtgcagtg tcagttgttg
     2701 aatgatcatc ggttctgacc gcccccgccg ggcttgggaa cctatcacag tgttggttac
     2761 agaatctcgc ccgcctcggc aaccttcttt tggggcaaac gcgcgcctgc acgcggtgat
     2821 ccaggccgtg aacgcgttgg aggcagggtc agcgagccgc gtaagctccc ggatgttgcg
     2881 ctcgccacgg atgttcgtac ggtcccatgc aggagagcgg gatcggttcg aacagcaggg
     2941 cacgcacggc aggcgcctgg gcggctgcac cggcgctcga caccgcctcg agatcgagcg
     3001 gtcgatcgct gctggcgttg agcgccgtct gttgcgtgtt gccgtcgcgc aggaactcct
     3061 ggccgcagcg cacgaacaga ttccgtgtga cgatgttgac gtcgggggcg gactcgagcc
     3121 gcgcgagttc gggatatcgg gccacatggg cgggatcggc ggcctgggga atgaactttg
     3181 cgatcgcttc gagccagcgc ttctcgcccc agcgcgagaa gctgatcccg gcgaaacagt
     3241 cgatgaacac gttgccgtcc acccagttgt ctttgccacc gtgtatctgc acgccgccaa
     3301 aggcgaccgc gccgcatcgt tcaaacacgt tgccctgcac gacggttccc gaaatcatgt
     3361 catcgaggcg cacgccggca gcgccattgt gcgtcccgcc gcggatgtcg ctccaccggt
     3421 tccagcgaat cacggtgccg cggtacagtg ggttgccgaa catgtcaatg cccccctggt
     3481 cgtccgactc ctgcaccacg tcgcggatca cgttcaactc gaccagatga tcgttgcctt
     3541 cgatccgcat ggccgaggat ggcatgcgct cgaacagatt gtaggccacg cgctgtccgc
     3601 agccgtccag atggaccgca ggggtgtagg tccgcttgag acgcgagatg ttcgacacag
     3661 tgcagttctc gacgaagtgc cgtccagccg cgagcgtctg ccggttgccc ccggcgacgc
     3721 gcgctccgcc acatccgagc gtctcgagaa ggcatccgaa caggccgtgg cgctggcctc
     3781 cctcgatgac gaccccgtca ccgcccagcc gccgaaccgt gcaacctgcc accaggcagt
     3841 tggtcccacc ccgaacctca ataccgttgg cgcgtccgtc ttgcacggtc aaccccagga
     3901 ggagcacgtg gtttgcgttg tccagggcga tgaaaggccg atccagaacg ctcaacacga
     3961 cctcggcctt ggccacatcc acgttttcag gcggcagcca gaacaccttg ccgcgctggc
     4021 gatcgaggta ccattcgccg ggctggtcga gttccctcaa cacgttcaac gcatagtacc
     4081 gctgtcccga ccgatacccg tactgggaat agggctgcgc cagcgtgtag ctgtgctcgg
     4141 acgggtcgat acgttcgact ttctggcact cctcgtacca gtcccagaac cagtacccat
     4201 agaggcgcac gtccggttcg tccgcccagg tgctcggacg ctcctcgatg tatcggaacc
     4261 ggccgtcgcg gcagccagcg atcgagttcc agaccttgaa ggtgtcctgg ccgaggagtt
     4321 ctccggtctt gacgaaacct tcgtcaggcc atcgcgcgag tgtctgcggc acgccgttca
     4381 cgaacaactc cggtcggcgc cgcagatctg tcggatcgcc gtagtccact acgcccaacg
     4441 cccccagatc ggctgccagc acacggtcgc gggccgatgc ggccagccgc tcgcgcacct
     4501 gcaggtcctc gatcggtgtc caattcgcaa tgcgaatccc accgctgaag acaggcgttt
     4561 cgccttctcc agctcggtaa acgaccggcg catcgggtct gccggaatcc tccgtgttca
     4621 gcgcgaacgt ctccgacacg gcatacatcc cgccatgcac gacaaccgtg acgcctcccg
     4681 atggcagcgg tccgcctcgc gcctgcttga gcgtgcgcac agcgtcgcgg gctcgatgca
     4741 gcgtcagaaa ggggttcccc tcggttccgt cgttgctgtc attgccgttc ggcgccatgt
     4801 ggaagagcgc tgtcgatcga gacacgtccg gcagccgcac gcggtcagac tcggcctcgt
     4861 ccgcggaatc gtcttcggct tggcggcttc ggttcgcctc ggtgaggcgg cgcaaagccg
     4921 cgtcccgata gagcgtcggc accgtggcat ccgccgccag acgcttccgg agaccctcga
     4981 tatcgtccga cgactcgccg gcaagagcca gatcagcgag gccgcaccag gccagaccgc
     5041 gaacgaatgg gggggcagaa gcgtcgcgcg caatcgcatc gaatccgtcg cgcgccttct
     5101 gggtctggcc gtcggcgagg gctttcgccg ccacttcgag cgtcggggac gcagtcccgg
     5161 ggagggtgga cagcttctcg aggaacgcat gggcgccgac gctcgagggg gtgatgacga
     5221 ccgtgacttt gccgtcgcgc acggcctcct tgagccgcaa gccgatgcgc gtagctgtgc
     5281 ggcgggcgac cagatcttcg cggatcggct cagcccggat gtcgagaggg atgttgtccg
     5341 gcacgctgat ctcgactcgg acagcctcgg cgccgtcggt gatctgcagc actcgagagg
     5401 acagttgttc gcatcgtccg aaggtcagca gggccgtttc gaacgtctcg gcctgggcaa
     5461 aagcgaactc atcaatcacg gtcagagacc cggagccggt ccggctgtag gtgcaggtgc
     5521 gggtgagctt gctgagcgag ggcacgtcgt aggccgcggc gaggtcgaac acgaccttgt
     5581 cggtcccttc atccgattgt tcgagcgcca gcagccgggc ggcggcctgg cgtccgggtg
     5641 actgcatctt gccggcgatc agcggcacgg ggtggccaaa cgagttcagg gcccggctct
     5701 cgtagcgtcg cgcgctgaat gtgcgctgcg tgtagacctc cgacccgatg tcgggcagca
     5761 cggccacgcc tcccacgacg gcgatgaatg tcccgacgtc attgtggtta tggtgctcgg
     5821 cgttgtggcc ccccttgatc gccacggcca ggcgagttcc cgcgctcgat ccaggtcggc
     5881 agatcagcac gcccgcgtct ggaaaccatg tccgcggtgc gctggccgtc gggatcggct
     5941 cgctgagcgg gatcggcggg gccggggagg gaaacaggtc catgagggcc gcctggaacg
     6001 atccgcgtgt cggcggtgcg agcgctgtgg gctcgtcggc gagtccgaag cgccggcgca
     6061 gcaagccgac caggcgcgat ccggggcggg cgcccacgtc gcagtccgca aatgcggggc
     6121 agacgccgtc ggcgatctcg atttggagtc catagagcgc gggcttgatc gctttcgggc
     6181 gggccagcag gtcgatgccg ccgccggtgg cacgccgaac cgcctccgcg agggccacgt
     6241 agttgccgaa gccgtagttc caatatccga cgccctcgct gcagtagccg tcgggggtga
     6301 atccctcgag aaaattggcc gagtatttct cggctgcaag gacgaaccat gctcgttcct
     6361 tcgggtcatc gagcgaggcc agcgcggcgc cggtcacgcc ggccaggcag acggcattcc
     6421 aattgtgcac gtgcagcagc cagaactgat cctgttcgcc cgccgtcatg cggcggtagg
     6481 gggcaaacac gcggcgttcg agttcctgcc gtatggtagt tcgcagggat ggcgagagct
     6541 tggcggccag gacccgatcg atcgtggaca gttcccaggc gaacatcgcg actgcgaggt
     6601 cgatggtgac ctggcgtcca tggaaattgt cgagccctcc gtcgtgcgcg ggcatgaccc
     6661 atgtgcgttc ccgggcgatg gactggaggg tggcttcgag cggttcgagg aaacgccccc
     6721 ggttctcgat acactcggcc agggccaatg aactcgcccg ggcacgccgg gcgaacaaca
     6781 ccgtttcgca gcgccgtcgg ttcccggtct tcgagtaatc gaggaacagc gcgtccggca
     6841 gatcggggag cggagtgcgc atttcctcct cggcatgccg gacgtgctgc tgcatttcag
     6901 gccggctcgc gatcctgtcc cacgcttcgc ggtcggtgca tgccgggccg aaagcgccag
     6961 gctcggacgg caactgtgcg gcgactctgg tcagcgcggc cgggtcgggt tggaacagat
     7021 cggcggcgcg gagtccgctc agacccgtgc aagcgacaag gaacgcggca accgatgacg
     7081 tggctgatcg aaacgagcgc ataaatggtc tgtgacttga atgcgagaat ctttgccacg
     7141 actaacaccc ttttgccggg gccgcaacgc tgttcgcttg gctttgcgcg gttctcgttt
     7201 tggccacgag cccggacatc cgatcgtgcg cagcggtgtg agatgtccgg actaaaggcg
     7261 acacatcaat cgtgcaattc gagacattca gcctcaccaa cgacaccctc ggggccgcct
     7321 cgccggccgc cgcaaccggc ctcgccgcgg ccgacgccga gagcggctca ttgaatctcg
     7381 cgtggacgcc cggcaacggc acggcgggca gcgtggtgat catgcggccg agcgccccaa
     7441 tcacccgtca accgatcgat ggggtcgcgt acaacggcaa tgcggtgttc gacgacgaga
     7501 agaaggaact gatctatggc acctgatcgc gggttgtcga caccgcggcg gcgggcatct
     7561 gcaaaccgcc cggtggctgg ggaacgcaca ggatcgggca tggcgtatcc gccagtatcc
     7621 ggctgatcgt ccggagggcg cgcagtcgcc gccaccctga gaactgctgg gtccccatga
     7681 caatcaggtg cgcccgcata tgccgggcgg cgcgcaggaa ggtcgacgcg ggactgccca
     7741 cacccacaca catgtcaagg tgcacgtcgg gaggagcatg gcgatgcacc caggtgcgaa
     7801 accggctctt ggtctgaacg aagcgccgat tgcgggcacc ggtcggccgg tagtgggagt
     7861 acagccgccc ggtgcgcagg acattgaagg ccgtgactct ggcattgaac caacgggcca
     7921 gagccagtcc ccgggccagg gcgttttcac ccaccctgct catctccgtc ggtatcagga
     7981 tcgtgtgcca gtggtccgga gaaaccagcg gcaaagactc ccccgactgc cacagcacct
     8041 ggggggggat cgcgagcacg ggacacgagg cgccaaagac aagacggcgt gtcacgccct
     8101 ggtcgacgaa gccctccagg ctcggcttcg accgagtgtg caggacgagc gcgtcgacgt
     8161 cgtatcgtgc cgccgcctcg agaatctccc tgtgaggctg gccatcgcga atccagacgt
     8221 tccagggaac ctgccaatgc actttgcgct tgatgacggc cagcaacttg gcgcgcgccg
     8281 cctccgccaa atcctccctg tccaggccag ccagcagcgg aaccgcccca acccgggccc
     8341 cccgcggacg cgtggtcaac acgtggacga tgtccagaca cgcgctcaac gactgcgcaa
     8401 gcttggaggc gtaggagagc gtcttcatcg actccaacga aaagtccacc ggcacaagaa
     8461 ttcgcttcca aggtgccatg ggatcgaggt tcgacctaga accgtcggcg agttcaggcg
     8521 tcggccagtc ggtccgggac acagaggtgt ccggtccggc agcttgatca atcagggcca
     8581 tgacgatgtg gttcttggtt cggtgtgatc tgcaccggtt tgatggcaat cactgtcagc
     8641 acggcatcgc gaccttccaa ttgagcacgc acggactccg ccaggccagg ctctggcttc
     8701 ggcatatggt gtttgaaggt cagtgccatg gtcgttagtg ttgttcagat tgctggtgcc
     8761 ttgccagggc cgcggcattc tcgcacggcg gtcgcctggg ttccgcacgg agagcactca
     8821 cgctgggacc tctccggcga agggctgcgt gccaggtagc gcgccaccgc tcgcgtgcgg
     8881 tctttctaca gaccggcaag gcaccgactt cagtaagctg gcgccgtgcc aatgtcgcct
     8941 gagcctgaat cccccacgaa cacgctcgtt ttcatgcatt ttgagacgca ccggctcgag
     9001 ttcatgggga agaaccatgc cactggtggt tcgcaccgtg cctgctacga tccaccgcgc
     9061 gtgctcgcgg catggcacgg cgcgaggtca ccggccggct cgcatcgaca acgaccctgc
     9121 gccctcagag agccgtcaag gccggtgcac gcgccgcccg gcggcgctcg cccaggacgc
     9181 tatgcaactg ctgacccaag aaggtcagag aaagcagacc atgctgcagt ctcacgaaat
     9241 tggcctccat gagaatcgtg gtcaccttgc gcacagcaac ctccggcaaa ggctgcctgc
     9301 tcttctcgat caactgggcg ttcacggcgg cggtgatgtc ggtcatgtgc atttcgttgc
     9361 cgttgcgcgc caggacctcg agaatccgac acgagaggga agactgcgcg aagtgccgca
     9421 acagaccctc acccaggctt tcagagggca ggagcgtggg gacgagcccg gacgtcgtgt
     9481 cggactcaag acgcggctcg tgcatgatgt cgagcaggtc cttgaccagg cgatcccgat
     9541 tctgcggagc aacgaacagt cgcaagatga tctccgttgc caggaggcag gcgacggcaa
     9601 tgagcgcgat gagttcggcg gtgttcatag cggagaggag tccaatcata attccattgc
     9661 ccgggcgtat gcgagcccgc ctgctgcctg gccgttcctg gcgggccttg ctcgcaaggc
     9721 ccaaccacgg tgcactaact cagcgggcgg gcggaccgtg gtccaggatc accacgggca
     9781 ccatcgacaa cttcttaacc cggatgtttc atagagcttc gcggagtctc gcatgcgggc
     9841 tctggcaccc gactaccctc gccttaagct ggcagtgtgc caagcaggcc aatgccggga
     9901 ttgaggctcg gacagcgtgg ttttgcgtgc ttagtgctta cgaagacggc tgtttcctgg
     9961 ctcacccctg accactcctg gttcgcgccg tgccacggca ggccgactgg caccgcgcag
    10021 ttcaatgcct catgtaggac cgccacaatg gcatcgcgca catcagccgg tcgtcaatgg
    10081 gcaacacgtg cggtcaaagg ccgaacgcgc agagcgcaaa aggccgccaa accgaggaac
    10141 acca
//
LOCUS       DAOQRO010000089        10063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6003_length_10063_cov_4.460132, whole genome shotgun sequence.
ACCESSION   DAOQRO010000089 DAOQRO010000000
VERSION     DAOQRO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6003_length_10063_cov_4.460132"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..187
                     /locus_tag="PKK20_04935"
     tRNA            97..187
                     /locus_tag="PKK20_04935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:131..133,aa:Ser,seq:gga)
     gene            249..1010
                     /locus_tag="PKK20_04940"
     CDS             249..1010
                     /locus_tag="PKK20_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99264.1"
                     /translation="MKTLLFALTGLVALAPLMRLSGLAADAAAADKQIDAVLDRYIEA
                     MGGRAAIEKVTTRIIKASFEGPGMPAALDWTQYAKAPNKQLAEIDIAGMGKMLDGFDG
                     QIAWSKNPFAGLRLKEGDELAKQKRDAEFYRDLHLRTVYTNLTYKGTEKVNGADVSVL
                     EAKPSAEALERWYFDATNGLLVRQDSEFNTAEGRMKLHMLFSDHRVVGGIKYPHLMRI
                     RTDIPGQPGAEFSIKVKEIKHNEAIDDSKFAKPGA"
     gene            1554..2360
                     /locus_tag="PKK20_04945"
     CDS             1554..2360
                     /locus_tag="PKK20_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNU99265.1"
                     /translation="MNKYWHVVGVGIQNTLVYRVNFLFRACFALIPLMATIYLWRAIF
                     ASNADSPQIAGYSLATMTSYYLLATIVDALTAVAEDDWQIAADIKDGRISQFLLKPID
                     YLAYRLCLFGAGRIIYAAVAALPATLFIVVQRSHFLLPSDGLTLVLFLASTVLTALLQ
                     FLMSYTIALLAFWVLEVSTFIFILFAFEYIAGGHLFPLDILPSALANALSYTPFPYQL
                     YFPVSIYLGRIAGDELWRGLLIQSFWVATLFGLARFAWHRGIRRYAAVGG"
     gene            2383..3222
                     /locus_tag="PKK20_04950"
     CDS             2383..3222
                     /locus_tag="PKK20_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNU99266.1"
                     /translation="MNASSHSRAFAGWGLLRRYARLYGALWRNSVVREMGFKTNFVLW
                     IFVEMLWFALQIAFIAVIYQHTDRIATWSKWQVVMLIGASHFIQQVFTAVFLLNCSNL
                     SELVRTGRLDFLLLLPINTRFLVSFRQVDLGAFVNAASAVAVMIYAARQLDLEPGIVQ
                     VLAFLALCVAGVLIHYFLMFLLATICFWTVRAQGIVWGYYNLFTVARLPDGAFRPGVF
                     KTVFTLALPMLLVSNVPVKLLVGMLESPLEIALLLGMACVCFVISEAGWRASVRQYTS
                     AGS"
     gene            3311..4363
                     /locus_tag="PKK20_04955"
     CDS             3311..4363
                     /locus_tag="PKK20_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU99267.1"
                     /translation="MEPSESGRELVFDALRGQPVARIATGPLAVHYCARFAGATLRQY
                     ATDARVLAESVIRYSERFKPDAVWLSADTWVSAEAMGVPVGATADNQPFGGLGPPYVR
                     SARDIGKIPRPDPGGQGRYPLMLEALTRIVKALGPGVCVVACLDQYPFSLAAALAGLE
                     QTMLRLVDDPPFVEALMERCLEYGLAYGCALASAGADILSGGDSPAGLIGPQLYRERV
                     WPFEQRLIAGLKSMTGKPVSLHICGDSTPLLADMTATGADILEIDHQVDLGQACRAVG
                     PNVALWGNLDPVAVLAQGSVAQVEAAARQAIETVAACGHSRFVLSSGCTLAVETPPEN
                     LDAMLEIARQRTSHRA"
     gene            4477..5775
                     /locus_tag="PKK20_04960"
     CDS             4477..5775
                     /locus_tag="PKK20_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99268.1"
                     /translation="MSKTSPLRLAFLGCGARGTTYAQLAARRPDRYLIVAGADPASER
                     IERIRAVCSNPDFRGFADGAAMLAAGKLADVLVIATQDHGHFEPCRRGLELGYNILLE
                     KPIATHIEEVLEIERLAAQTGRRVMVCFVLRFAAFYRKVKEIIDSGRLGEVSGIQASE
                     GVMPWHQAHSFVRGHWSVVAKSSPMLLSKCCHDTDVLHWLVGRRCLRVASFGSLEFFR
                     RERAPAGAPQRCTDGCPVAENCPYNALRYTGDRRVPWLGMVYDRAASATPQEIVDWLR
                     TSPWGRCVYRCDNDAVDRQVLALEFEGGITGTFTMTAFENGRHIEVYGTKGVLKGGET
                     YRHHFGAHLVFLPHEGAPERYTVQAEDGGYELHGGGDPGLINALYDEMTRPAGEPLQA
                     GIDSTVHSHVLTFAAEEARLTRRTVDVAEFARRIAPLRAG"
     gene            complement(6081..7130)
                     /locus_tag="PKK20_04965"
     CDS             complement(6081..7130)
                     /locus_tag="PKK20_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99269.1"
                     /translation="MWLQSPARAAQYRAAGINTYVGLWRGPTEEQLAALRATDMKVIC
                     DQNAVGRRHLDDPTIIGWMHGDEPDNAQSLGEGKGYGPPILPEKIVEDYERIRAVDPT
                     RPVLLNLGQGVAWDGWHGRGVRTNHPEDYPQYVKGGDIVSFDIYPVVHDRPAVTGKLW
                     FVARGVERLVSWTEGRRIVWNCLECTRISHPTAKPTPQHVRAEAWMSLIHGSRGLIYF
                     VHEWQPKFNEAALLSDPEMLAAVTALNKQIHRLAPVLNQPPLADGARVVTDNPEVPVA
                     TTTRRLGDALYVFAVGMRDGSTTATFTVKEWPDQASVEVLYEDRALPLGKGAFRDTFK
                     PWEVHLYRIATRPDR"
     gene            complement(7620..8921)
                     /locus_tag="PKK20_04970"
     CDS             complement(7620..8921)
                     /locus_tag="PKK20_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99270.1"
                     /translation="MSQSSNLSRRSFLRTATATALATPLLLPGRIWSAESGAQPNSRV
                     AMGFIGVGTQGRHLLNSFLPHGNIQVVAVCDVDTTRREHHKQKVEEFYAAKQDKTFKG
                     CAAYTDFRELLERKDIDAVVIATPDHWHAYPVVAAANAGKDIYCEKPLSLTIHEARAM
                     VNAVRKNERVFQTGSMQRSDGAFWKGCTLVRNGKIGRIKEVYANVGGPSKWCDLPEEP
                     MEPGLDWDLWLGPAPQRPYNSVLSPRGVHTHFPNWRGYREYSGGGMTDWGAHHFDIAQ
                     WGLGMDESGPVEITPPDGKDIKQLRYTYANGSVLIHGGLKGYNFGVVFVGTDGKVCVD
                     RGRFKAEPESLEKENFDALPVQLYKSNNHYKDFVDCIRTRKRPICDVEVGARSVTVCH
                     LGNLAYWNTRALKWDPRTEQFIGDSEANTWRDYEERDPWKV"
     gene            9077..9943
                     /locus_tag="PKK20_04975"
     CDS             9077..9943
                     /locus_tag="PKK20_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99271.1"
                     /translation="MSAIAVTRNSPVPRLQASRLGFTLIELLVVIAIIAILASMLLPA
                     LGKAKLKATFASCASNQKQLVLAWQMYALDSDDTMLPTSYRGELGQMELYAGGFWNGP
                     NPSIASGITETEAMKRVTAGIAMSPLYKYCSAIGSWHCPGDTRTKRNKPGRGWAYDSY
                     SKADGMNGGMWTGIKPYTKTTEISEPVNSFVFIEEADPRDYNNGTWVLDVTPPGWVDP
                     FALFHGNISTFGFADGHVESHRWLETSTIKAASDSARGIQSFYWSGGNAKNRDFVWVW
                     NKFRHLNWKPLN"
BASE COUNT         1847 a         3061 c         3160 g         1995 t
ORIGIN      
        1 ggcgcaacct gccgatcgag agagccttgg tttcgagcga gcgccgaatc agcttgccct
       61 gaacccgaac ccgtgcgaat aaagtccccg atggccggag agatggcaga gtggtttaat
      121 gcgcacgcct ggagagcgtg agtgggctta taccccaccg ggagttcgaa tctccctctc
      181 tccgccattt ccaaagaacg gtccttgatt gacacacccg gcacagcgcc gccttatcgc
      241 atcccatcat gaagacactg ttgtttgcac tgactggact tgttgcccta gcgccactca
      301 tgcgcctgtc tggcctggct gccgatgccg ccgccgcgga caagcagatt gacgccgttc
      361 tggatcggta catcgaggcc atgggaggcc gcgcagcgat cgagaaggtg acgactcgga
      421 ttattaaggc ctcgttcgaa ggcccgggaa tgcccgcggc cctggattgg acccagtacg
      481 ccaaagcgcc caacaaacag ttggcggaaa tcgacatcgc gggaatgggc aagatgctcg
      541 atggctttga cgggcaaatc gcctggtcca aaaacccctt tgccgggctg cggttgaaag
      601 agggtgacga attggcaaaa cagaagcgcg acgctgagtt ctaccgggac ctgcatctcc
      661 gaaccgtcta cacgaacctg acctacaagg gcacggagaa ggtcaacggc gccgacgtgt
      721 ccgtgctcga agccaagccg tccgctgagg cgctcgagcg ctggtatttc gatgccacaa
      781 atggcctcct ggtgcgccag gattccgagt tcaacacggc ggagggccgg atgaaactgc
      841 atatgctctt ctcggatcat cgggtggtgg gcgggatcaa gtacccgcac ctgatgcgca
      901 ttcgcaccga catcccggga caaccggggg cggagttctc gatcaaggtg aaggagatca
      961 agcacaacga ggccatcgac gactcgaagt ttgccaagcc cggagcctaa cccgaaagtt
     1021 tcacctgtgg ggcaatcgca gcattccgga tggtctgcgg ctgagatttc cgggtctgtg
     1081 aaagccagca tttcaggtga atatgacggt gaccggtcag atgacatcac ccgttggcga
     1141 gcaatgcggg cgagctcgca tctttcacac cccgatggtg cacagggcac tcagacttcg
     1201 caggaggact gctgctatgc gggctcgaga agtaaaactg cgccaggatc gagagacaga
     1261 taactgcacc acactgttcc catgagagac cattctcagg accgacagaa tgctcccggt
     1321 tcccttgctg ggcgcggttt tgccagagcc cggatgtcag actccgcgaa gctttgtgaa
     1381 acatccgggc tagccggcct attccacaaa cctcgaccgc cgcgaggcac tgtctgggaa
     1441 tggcgcagtc accaggtcag tgatccgggc tttctcctgc agccgcggca tcggctctcc
     1501 gatcttcggt ttcgcggcgg ccacaacccc ctgaagcatc cgggctggac gcggtgaaca
     1561 agtactggca tgtcgtgggc gtggggattc agaacaccct ggtctacagg gtgaatttcc
     1621 ttttccgggc ctgttttgca ctgattccgc tgatggcgac catttacctg tggcgggcca
     1681 tcttcgcgag caatgcagac agtccgcaga tcgccggcta ctcgctggca acgatgacgt
     1741 cgtactatct gctcgcgacg atcgtcgatg ccctcaccgc ggtggcggag gacgactggc
     1801 agatcgcggc tgacatcaag gatggccgga tcagccagtt cctgctcaaa ccgattgact
     1861 accttgcgta ccggctctgt ttgttcgggg cgggtcggat catttacgcg gcggttgcgg
     1921 ccctgccggc gaccctgttc atcgtggttc agcgctcgca ttttctcctg ccgtcggatg
     1981 ggctgacgct cgtgttgttc ctggcctcga cggtcctgac ggcgctcctg cagtttctga
     2041 tgtcctacac gatcgccttg ctggcctttt gggtgctcga agtatcgacg tttatcttta
     2101 tcctgttcgc cttcgagtac atcgcgggcg gtcacctctt tcctctcgac atcctgccgt
     2161 ccgccctggc caacgcgctc agttacacgc cattccccta ccagctctac tttccggtca
     2221 gcatctacct gggccgcatc gccggagacg aactgtggcg cgggctgttg attcagtcgt
     2281 tttgggtggc gacgctcttt ggcctggcgc gcttcgcatg gcatcggggc atccggcgct
     2341 acgcggccgt gggcggttga gcactgcgca aatcttcgag tcatgaacgc ctcgtcgcac
     2401 agcagggcct tcgcgggctg ggggttgttg cgccgttacg cccggctgta cggggccctg
     2461 tggcgcaact ccgtcgtgcg cgagatgggg ttcaagacga acttcgtgct ctggatattc
     2521 gtcgagatgc tctggttcgc cctgcagatc gccttcatcg cggtgatcta ccagcatacg
     2581 gatcggatcg cgacatggtc gaagtggcag gtggtgatgc tcatcggcgc cagccacttc
     2641 atccagcagg tcttcactgc cgttttcctg ctgaactgct cgaatctctc cgagttggtc
     2701 cggaccggaa ggcttgattt tctgctgttg ctgccgatca acacgcggtt tctcgtctcg
     2761 ttcaggcaag tcgatttggg cgcattcgtc aacgcggcat ccgccgtggc cgtgatgatc
     2821 tatgccgccc ggcagttgga cctcgagcca ggcattgtcc aggtcctggc cttcctggcg
     2881 ttgtgcgtgg cgggcgtgct cattcactac ttcctcatgt ttctcctggc gactatatgc
     2941 ttttggaccg tccgggcgca ggggatcgtc tggggctact acaacctgtt caccgtcgcg
     3001 cggctgcccg acggcgcgtt tcgacccggc gtgttcaaga cggtgttcac cttggccctg
     3061 cccatgctgc tcgtgtccaa cgtgccggtg aagctcctgg tcgggatgct cgagtcgcct
     3121 cttgagattg ccctgctgct cggcatggcg tgtgtctgtt tcgtgatctc ggaggccggc
     3181 tggcgagcct cagtacgcca atacacaagc gccggatcct gactcgaggc agcgacaaaa
     3241 gtgacgacag agagcgatgg acttgtgatg cacagcgcgg cagagcccga tcggcaatag
     3301 gaaatgtgca atggaaccat ccgaatcagg tcgagagttg gtgttcgatg cgctgcgcgg
     3361 gcagccggtc gcgcggattg cgacaggtcc gctggcggtc cactactgcg cgcggttcgc
     3421 cggcgcgacg cttcggcaat acgcgaccga tgcccgtgtg cttgcggagt cggtcatcag
     3481 gtattccgag cgattcaagc cggatgcagt gtggctctca gctgatacct gggtcagcgc
     3541 cgaggccatg ggcgtccctg tgggagcaac agcggacaac caaccgtttg gcggactcgg
     3601 accgccgtat gtccgaagtg cacgcgacat cggcaagatc ccacggccag atcccggcgg
     3661 gcaggggcgc tacccgctga tgctggaagc gctcactcga atcgtcaagg ccctggggcc
     3721 gggcgtttgc gtggtggcct gcctcgatca gtacccgttt tcgctggctg ccgccctggc
     3781 cggtctcgaa cagacaatgc ttcgcctggt ggatgatcct ccgtttgtcg aggcgctgat
     3841 ggaacggtgc ctcgaatacg ggctggccta cggatgcgcc ctggcttcgg caggggcgga
     3901 tatcctgagc ggcggagatt cgccggcggg cttgatcggg cctcagctct atcgcgagag
     3961 ggtgtggcca ttcgaacagc gtctgattgc cggattgaag tcgatgacag gcaagccggt
     4021 gtcgcttcac atctgcggcg actcgacgcc gctgctggcc gacatgactg ccacgggcgc
     4081 cgacatcctc gaaatcgatc accaggtcga cctcggccag gcgtgtcggg cggtcggacc
     4141 gaatgttgcg ttgtggggca acctcgatcc ggtcgcggtc ctggcgcagg gcagcgtcgc
     4201 ccaggttgaa gcggccgccc gccaagcgat cgagaccgtc gccgcgtgcg ggcattcgcg
     4261 gttcgtcctg agttccggat gcactttggc ggtcgagacg ccgcctgaga atctcgatgc
     4321 catgctcgag atcgcgcgac agcggacgtc acatcgcgct tgatcgagtt cggttccagc
     4381 ccgcatgttt catggacttg tgactatagc gaagtcgaag actccaacgc gttgaccaat
     4441 ccgggctaga gaggccatga acaccgaaga caatctatga gcaagacctc tcctctgcgg
     4501 ttggcctttc tgggctgcgg ggcccgaggc accacctatg cacaactggc agcgcgccgg
     4561 cccgatcgat acctcatcgt ggcgggcgcc gacccggcgt cggaacggat cgagcggata
     4621 cgcgccgtat gctcgaaccc ggatttccgc ggctttgccg acggggcggc catgctggcc
     4681 gcggggaagt tggcggacgt tcttgtaatc gccacgcagg accatgggca tttcgagcct
     4741 tgccgacgtg gtctcgagtt gggctacaac atcctgctcg agaagccgat tgccacccat
     4801 atcgaggagg tgctcgaaat cgagcgcttg gcggcgcaga caggtcggcg ggtcatggtg
     4861 tgttttgttc tccgttttgc agccttttat cgcaaggtga aggagatcat cgattccgga
     4921 aggctgggcg aggtctcggg catccaggcc agcgaaggcg tgatgccgtg gcaccaggcc
     4981 cattcgtttg tgcgcggcca ttggtcagtc gtggccaaga gttcgccgat gctgctctcg
     5041 aaatgttgcc atgacaccga tgtgctgcat tggctcgtcg ggcggcgttg cctgagggtg
     5101 gcaagtttcg gctcgctcga gttctttcga cgcgagcggg cccccgcggg cgccccccaa
     5161 cgatgcacgg acggttgtcc ggtggccgag aattgcccat acaatgcgct gcgctatacg
     5221 ggcgaccggc gcgttccctg gctgggaatg gtgtacgacc gggcggcgtc cgcaacgcct
     5281 caggagattg tcgactggct gcggacaagc ccctgggggc gatgcgtcta ccgctgcgat
     5341 aacgatgcgg tggatcgcca ggtgctcgcc ctcgagttcg agggcgggat cacggggacg
     5401 ttcaccatga cggccttcga aaacgggcgg cacatcgagg tctatggcac caaaggcgtg
     5461 ctcaagggcg gcgagaccta ccgacaccat ttcggggcgc acctggtctt cctgccgcac
     5521 gaaggggcgc ccgagcgcta cactgtccag gcggaggacg gcggctacga actgcacggc
     5581 ggcggcgatc cgggcctgat caacgccctg tacgacgaaa tgactagacc ggcgggcgaa
     5641 ccgttgcagg ccggcatcga ctcgacggtc cacagccatg tcctgacctt cgcagccgag
     5701 gaagcgcgat tgacacggcg gactgtggac gtggcggagt tcgcccggag aatcgcccct
     5761 ctgagagcgg ggtgacacaa aaaagaagca cccactcggc agtctttgtc gctgcgtgcg
     5821 tcgccaactt tgtggtcaag cggcgtggcg gtcgacaaag gcaccgacaa agattcgcga
     5881 caaaggcggt gtcgtggcga accccattgt cattcgaggc ggtgtgtcag aatggccggg
     5941 ggttcgtggg gaagcgttga cattcaacag ttcaatgcgc acggcgacca tcaactggag
     6001 tctcctgctc ctgtccatga accgcaaacg ctcaacgtcc agctctcaac tcccaaccct
     6061 taactctcga ttgacctggt ctaccgatcg gggcgcgtgg cgatacggta gaggtgcacc
     6121 tcccacggtt tgaacgtgtc gcgaaatgca cccttgccga ggggcaacgc gcggtcctcg
     6181 tacagcacct cgacgctggc ttggtctggc cattccttca cggtgaaggt ggcggtggtc
     6241 gaaccgtcgc gcattcccac agcaaaaacg tacaaggcat cgcccaggcg cctggtcgtc
     6301 gtcgccacag ggacctccgg attgtccgtc acgacgcgcg cgccatcggc gagtggcggt
     6361 tggtttagga ccggcgcgag ccgatgaatc tgcttgttga gcgccgtgac ggcggcgagc
     6421 atctccgggt cgctgagcag ggccgcttca ttgaacttgg gctgccactc gtgcacgaaa
     6481 tagatcagac cccgcgagcc gtgaatgagc gacatccagg cctcggcccg gacatgctgg
     6541 ggcgtgggct tggcggtcgg atgcgaaatg cgggtgcatt cgaggcagtt ccacacaatc
     6601 cgacgcccct ccgtccacga cacgagtcgc tcgacacccc gggcgacgaa ccacagtttg
     6661 ccagtcaccg ccgggcgatc gtgcaccacg gggtaaatgt cgaacgacac gatatccccg
     6721 cccttgacgt actgtgggta atcctccgga tgattggtcc ggacgccgcg tccgtgccat
     6781 ccatcccagg ccaccccttg gcccagattc agcaggacgg gccgggtcgg atcgacggct
     6841 cggatgcgct cgtagtcctc gacgatcttc tcaggcagga tcggcgggcc gtagcctttg
     6901 ccttcgccga gcgactgggc attgtcgggt tcgtcaccgt gcatccagcc gatgatggtc
     6961 ggatcgtcaa ggtggcgccg tcccacggcg ttctggtcgc agatgacctt catgtctgtc
     7021 gcgcgcaacg ccgccaattg ctcctcggtc ggcccacgcc acaggccgac gtacgtgtta
     7081 ataccggcag cgcgatactg ggcggctcgg gcgggagact gcagccacac ggcgattgga
     7141 aaataatccg gcgaggccga aggaccattc ttccaggccg agtagtcggc gggaagactt
     7201 gctggcgcca ggcaaagcag aaagacaaac caaagaacca gtccggcaga acatggtcgc
     7261 cgcgtcggcc ttgtctctgt ccctgtcgcg cgggtcagcg cctcgaagaa catacggccc
     7321 gcggacctga gtttgctggg attccttacg accggcgcgc cgtcgtgcgg acgggagtgt
     7381 gcgtttgctg gtgagttact ccgagaacag acatcccctc ggcagtcttt gtcgttgcct
     7441 ccgtcgatat gcttgccgtc aggcgacgtg gcggttgaca aagccgacaa aggcgttgtc
     7501 gtttggaacc tcattttcat ttgaagcggt gtgccggaat agcccgggga acttatggag
     7561 agcgttgaca ttcaacgctc aacgtacact acacaaccat caaccatcaa ccaacgttgc
     7621 tacaccttcc aggggtcgcg ttcctcatag tcacgccacg tattggcttc cgagtcgccg
     7681 atgaactgtt cggtccgggg atcccatttc agcgcccgcg tgttccagta agccaagttg
     7741 cccaggtgac agaccgtgac cgaacgggct ccgacctcga catcgcagat cggtctcttc
     7801 cgcgtccgaa tgcaatcgac gaagtccttg tagtgattgt tgctcttgta gagctggaca
     7861 ggcagggcgt cgaaattctc cttctccagc gattcgggct cggccttgaa tcgcccgcga
     7921 tcgacgcaga ccttgccatc ggtccccacg aagaccacgc cgaagttgta gcccttcaaa
     7981 ccgccgtgaa tcagaacgct cccgttggcg tacgtgtacc tgagttgctt gatgtccttg
     8041 ccatccggag gcgtgatctc gaccggcccg ctctcgtcca tccccagccc ccattgtgcg
     8101 atatcgaaat ggtgggcgcc ccagtcggtc atgccgccgc cggaatactc gcgatacccg
     8161 cgccaattcg ggaagtgggt gtggacgccg cgcgggctta ggacggagtt gtaggggcgt
     8221 tggggcgctg ggccgagcca caaatcccaa tcgaggcccg gttccatcgg ttcctcgggc
     8281 agatcgcacc atttgctggg gccgccgaca ttggcataga cctccttgat ccgcccgatc
     8341 ttgccgttgc gcaccagcgt gcaccccttc caaaacgcgc cgtcggaacg ctgcatcgat
     8401 ccggtctgaa agacgcgctc gttcttgcgg accgcattga ccatggcgcg cgcctcgtgg
     8461 atagtcaggg aaagcggttt ctcgcagtag atgtctttgc ccgcattcgc ggcggcgacg
     8521 accggatagg catgccagtg gtcgggtgtc gcaatcacca cggcatcaat gtctttgcgt
     8581 tcgagcaact cgcggaaatc cgtgtacgcc gcgcatccct tgaaggtctt gtcctgcttg
     8641 gccgcataga actcctcgac tttctgtttg tggtgctcgc ggcgcgtcgt gtcgacgtcg
     8701 cagacagcaa ccacctggat gttcccatgc ggaaggaagc tgttgagaag gtggcggcct
     8761 tgtgtcccca caccgatgaa gcccatcgcg acgcggctgt tgggttgggc gccgctctcg
     8821 gcagaccaaa tgcggccggg cagcaacagg ggcgtggcca gcgcggtggc ggtcgcggtg
     8881 cggagaaagg agcgacggct gagattcgag gattgagaca tagataattg ttgggtctga
     8941 cgctcggcag ggcgggcgga ttcaaccgga gctcgggttc ccctcgtgct ctcaaacgaa
     9001 cacttgactt ccggcatctc ttgggacgta gttgcggcag aaatcaaccc gaatccagta
     9061 tgtgctgacg cgtgttatgt ctgctattgc cgtgacccgg aacagcccag tgcctcgcct
     9121 ccaggcgtcg aggctgggct tcacactgat cgaactgctg gttgtcatcg caatcatcgc
     9181 aatcctggcg tcgatgctcc tgccggccct gggcaaagcc aagctcaagg ccacattcgc
     9241 cagttgcgcg tccaatcaga agcaactggt cctcgcgtgg cagatgtacg ccctcgacag
     9301 cgacgacacc atgctcccca catcgtaccg gggcgaactg gggcaaatgg aactctacgc
     9361 gggcggtttt tggaacgggc cgaatccgag catcgcgtcg ggcatcactg aaaccgaggc
     9421 catgaagcga gtcacggccg gcatcgccat gtcgccgctc tacaagtact gcagcgcgat
     9481 cggctcctgg cattgtccgg gcgacacgcg caccaagcgg aacaaaccgg gtcggggctg
     9541 ggcgtacgac agttactcga aggccgacgg gatgaacggc ggcatgtgga ccggcatcaa
     9601 gccttacacc aagacgaccg agatcagcga accggtcaac tcctttgttt ttatcgagga
     9661 ggcggatccg cgcgactaca acaacggtac gtgggtcttg gatgtcacgc cgcccggctg
     9721 ggtggacccg tttgctctgt tccacggcaa catcagcacg ttcgggttcg ccgacggcca
     9781 tgtcgagtcg catcggtggc tcgagacctc gaccatcaag gcggcgtccg actcggcccg
     9841 gggcattcag agcttctatt ggtccggggg caacgccaag aaccgcgact tcgtctgggt
     9901 ctggaacaag ttccgacact tgaactggaa gccgctgaat taatcaggtc ggcaaatcca
     9961 ttcgaacccg ccacggtgac cgcccgccaa gggtcaaccg cgaaccacgc tgaacacgcg
    10021 gaaagaggag gcttgcagaa tccggcagta gtgcgtgagc ggc
//
LOCUS       DAOQRO010000090        10036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6021_length_10036_cov_4.802324, whole genome shotgun sequence.
ACCESSION   DAOQRO010000090 DAOQRO010000000
VERSION     DAOQRO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_10036_cov_4.802324"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKK20_04980"
     CDS             complement(<1..389)
                     /locus_tag="PKK20_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014093124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNU99272.1"
                     /translation="MLTTTSTEVKVDALLAAKLGLTEEEYASICRLLGRNPNFTELGL
                     FSAMWSEHCSYKNSRKLFRLFPTKGKQVLVGAGEENTGVVDIGDGFGVAFKIESHNHP
                     SAVEPFEASATGIGGCLRDIFTMGARPV"
     gene            complement(539..1441)
                     /locus_tag="PKK20_04985"
     CDS             complement(539..1441)
                     /locus_tag="PKK20_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNU99273.1"
                     /translation="MTIHPHRKHRTILAAIVLLPLILYAMLRWFEHKQVYHPSREWAE
                     TGRALGRPWEDVRLQTADGIELSAWFFAAHSNSLRSQLTLAVCHGNGGNISHRLDLYQ
                     ALLDTGVAVFAFDYRGYGKSAGRPGEEGTYLDAQAAYAWLRRRGFAGSNIIAFGESLG
                     GAVATELARREPLGGLILQSTFTSIPDIGAELFPWLPVRWIASIRYDTHRKLPELDIP
                     LLIMHSRADRLIPFHHAEANFAAARGPKRLWEIAGDHNDPLDADRAGFVDGLETFLAE
                     TFPVQATRPGREDGTGPAPSVTTP"
     gene            1799..2833
                     /locus_tag="PKK20_04990"
     CDS             1799..2833
                     /locus_tag="PKK20_04990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNU99274.1"
                     /translation="MATPDLLTRLLRDVSRSFYRTLRVLPSGVRPQIGLAYLLARITD
                     TIADTEIVSLEGRMAALDRLRTRIAIPRSGSVRLESFFADAGAGQGASAERDLLLRAD
                     EALGVLDAVEPGDGRLIRNVLRTIISGQELDLRRFRGASAQCPAALDTEEDLDDYTYR
                     VAGCVGEFWTHLCRARLFPAVRVDEGFLLANGVRFGKGLQLVNILRDIPRDLRHGRCY
                     LPSNRLAAHGMRASSLLDARNMPRFRKLYEDYLDLAGACLADGWAYTNTLPSRQMRVR
                     LACAWPILIGLRTIARLRVENVLDDRRRIKVSRGEVKRLMLRSVCVYPWARAWNRLFE
                     RARQMPSSVL"
     gene            2830..4980
                     /gene="ligA"
                     /locus_tag="PKK20_04995"
     CDS             2830..4980
                     /gene="ligA"
                     /locus_tag="PKK20_04995"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNU99275.1"
                     /translation="MTAASAKARHADLAAQIRVHDHAYYVLARPTISDREYDRLYHEL
                     LELERQWPELVTPDSPSQRVGGAPLAEFRPVRHAQPMQSLDNTYSQAEVKEFVGRIQR
                     LLPGEPLEWCVEPKIDGVAVSLRYENGRLAVAATRGDGTTGDDITANLRTIRSVPLVL
                     AHPRSELASRSAPGGTAKTGEECRRPTQGDLFGAGAAGGAGQAGPIPRVLEVRGEVFL
                     GKAGFARLNEEREALGEEPFANPRNATAGSLKQLDPRIVAARPLDIVLYGLGHVEGVR
                     APATQIELLDWLRALGLRTPQPRWLCRSLDELFEAIASLEGQRRDFAYQTDGAVIKLN
                     SVALRARCGSTSKAPRWAIAYKYAAEQAQTRLNAITIQVGRTGALTPVAELEPVFLSG
                     STISRATLHNEEELRRKDIRLGDWVVIEKAGEVIPAVVGVVLERRNGHEKQFEFPRSC
                     PECKSKVSRGSVLGEAGVVWRCVNPDCPAKIRGRIEHWCSRGAMDIEGGGEVLVAQLV
                     RNGLVRDVADLYRLRLDELAALERMGEKSARNFLAAIEASKRRELSRLIFGLGILHVG
                     ARVAKSLARRFATLDDLMRAGQEHLTATEDVGEIIAASVYQWCSDAENRRLIERLRKA
                     GLSFESTAFQAGSGRGPFAGKSFVLTGTLPTLTREQATAKIEALGGRVSGSVSKKTDF
                     VLAGADPGSKLAKAQKLGVRIIDEAEFRRLGGAG"
     gene            4943..5815
                     /locus_tag="PKK20_05000"
     CDS             4943..5815
                     /locus_tag="PKK20_05000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNU99276.1"
                     /translation="MKRSSAALEARADRMPKTDSIELGNGERLRILHEDRAVLAIDKP
                     AGWMLVPVSWQNTGRNLQAALLSCLASGAYWVRSRNLTFLRYAHRLDAETTGVLLLAK
                     SRGALDALGGLFESRLMQKSYLAVVQGRPPRDAWTCNLPLAPHPRKHGRVIVSRHDGK
                     STETRFRVVERKTDAQRGTWSLIEARPVTGRTHQIRVHLAADGLPVLGDALYGPRQSG
                     LMPDDFPLALRAVSLAYADPFTRRAVRIDAPRTLFLRAFGFELPAAGEPRGNSRRPPQ
                     PGLPAGRFVDTLGP"
     gene            5812..6381
                     /locus_tag="PKK20_05005"
     CDS             5812..6381
                     /locus_tag="PKK20_05005"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNU99277.1"
                     /translation="MIEPWPTLESKTLGDFRVFRVRQDSKRSPRTGQTHNFFVLECPG
                     WVNIIALTPHRDIILVEQYRHGVQTVELEVPGGVMDADDESPIETAVRELREETGFEG
                     ENAVVIGRVAPNPAIQNNIAGTVLIENCVRRHEVEFDHTEDVVTRIEKVDALPLLVAS
                     GRIRHSLSIAAFYHFDLWLRGLAPNAPRH"
     gene            complement(6454..6924)
                     /locus_tag="PKK20_05010"
     CDS             complement(6454..6924)
                     /locus_tag="PKK20_05010"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNU99278.1"
                     /translation="MSGAYMVRQLPPWFENVGKRVVKSPKVHVRDSGLLHALLGIPDE
                     AALSGHPKLGASWEGFVIEQILAWAGERNAYFWATHSRAELDLMVPAKGQRWGFEIKY
                     QDAPTITKSMRIAMQDLKLAKLWVVYPGPTGYPMDEQIECVSPAELGRVREALT"
     gene            complement(6957..7253)
                     /locus_tag="PKK20_05015"
     CDS             complement(6957..7253)
                     /locus_tag="PKK20_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU99279.1"
                     /translation="MNDGLAHSPGTLLLGPRQCGKTTLARMLGGVSSKNYYDLESPRN
                     LARLAHPQTVLEAARGLVVLDEIHQRPDLMPLLRVLADRQPPPCRFLLLGSASG"
     gene            complement(7346..7768)
                     /locus_tag="PKK20_05020"
     CDS             complement(7346..7768)
                     /locus_tag="PKK20_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99280.1"
                     /translation="MPIGTTMPETVRERWRTANRFADVLEAEVKMRRNPLAARVVSWF
                     NLCRVCQDLEEQMLLAPQPGEDDSQLHRALLSTAIASGEGLLLECDDAETLKPLRLTP
                     PALAAKVESLRVTFTQWHTELKPERQAAILKEAFRGTV"
     gene            complement(7905..9950)
                     /gene="brxL"
                     /locus_tag="PKK20_05025"
     CDS             complement(7905..9950)
                     /gene="brxL"
                     /locus_tag="PKK20_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HNU99281.1"
                     /translation="MELDAIDKLAAKAFEGYIVRKDLVRKFKGQYPVPTYVAEFLLGR
                     YCSSTDEKEIEEGLQVVQRQLADRTVRPGEEEYFKSKARETGSIRIIDIITARLDAKT
                     DSYVAELPSLRLRDVRITPALVQQHDRMLTGGFYAEIELTYDATIAAEQSGRPFGVES
                     IREIQLSKRDVLEAMYRGRELFNLEEWKRFLIRSIGLEPDALTPRNIDVVFLRMVPYV
                     ERNYNMVELGPRGTGKSHLFQQISPYAHLVSGGKTTVARMFVNMANGQRGLVCQYDVV
                     CFDEISGVSFDQKDGVNIMKGYMESGEFSRGKESIRADGGIVMVGNFDVDVQHQQKIG
                     HLFGPLPPEMRDDTAFQDRIHCYAPGWDVPKMSGQLFTNHFGLVSDFLSECWTHLRKQ
                     SRLTALQGRVFFGGALSGRDTWAVQRTVNGLLKLLFPNPDSPVTDEALEWAVRLALEC
                     RRRVKEQQKRIGSAEFRNTHFSYTLGQDGVEKFVVTPELQSEDSIGSDPLPPGQIWAI
                     SPGGLDENAGLYRIEVNEGPGSGVKILNRPAPAPFSESVRYAEQNLYSRAKQLTGDRD
                     PREHEFSIQLRAFDAGKNGTGTGLAVLVALCSALLQKSTKGGLVVIGGLNLGGSIDPV
                     HNAVNVAELAIEKGATTLLMPVSARKQLNDLPDDLITKVNILYYSDAREALLKAIVE"
BASE COUNT         1741 a         3238 c         3184 g         1873 t
ORIGIN      
        1 acgggcctcg cgcccatcgt gaagatgtcc cgcagacatc ccccgatccc ggtggccgat
       61 gcctcgaagg gctcgaccgc ggacgggtgg ttgtgcgact cgatcttgaa ggcgacgccg
      121 aagccgtctc cgatatcgac cacgccggtg ttttcttccc cggcgccgac gagcacctgc
      181 ttgcccttgg tcgggaagag ccggaagagc ttgcgcgagt tcttgtaact gcagtgttcc
      241 gaccacatcg cggaaaagag cccgagttcc gtgaaattcg gattccgtcc gaggagtctg
      301 cagatcgagg cgtattcctc ttctgtgagg ccgagtttcg ccgccagaag cgcatccacc
      361 ttcacttcgg tactcgtggt cgtcagcatc gcgaatgttg tacaccatcg aaaggacgag
      421 gcaagcggcg ttagcccgga ttcttcataa agctttgcgg cgtctaaaat ccgggctctc
      481 gctgtgcgtg caggacctcg cccccgggtg agggcacccg gcccgatcaa catcgctgtc
      541 agggggtggt cacgctcggg gccggtccgg tcccgtcctc ccgccctggg cgggtggcct
      601 gcacggggaa ggtctccgcc aggaacgtct cgagaccgtc cacgaacccg gcgcggtcgg
      661 cgtcgagggg atcgttgtga tcgccggcga tttcccagag gcgcttgggg ccgcgtgcgg
      721 cggcaaagtt tgcctctgca tgatgaaacg gaatcaggcg gtccgcgcgg ctgtgcatga
      781 tcagcagggg aatgtccaac tcgggcagct tgcggtgcgt gtcgtagcgg atcgatgcga
      841 tccaacggac aggcaaccaa gggaacagtt cggcgccgat atcggggatg cttgtgaagg
      901 tgctttgcag gatgagtccg cccaacggtt cgcgccgggc cagttcggtg gcgacggcac
      961 ctccgagcga ttcgccaaaa gcgatgatgt tcgagcctgc gaagccgcgt cgccgcagcc
     1021 atgcgtaggc ggcctgggca tcgaggtaag tcccttcctc cccagggcga ccggcgctct
     1081 tgccgtatcc gcggtagtca aaggcgaaca cggcgacacc ggtgtccagc agggcctggt
     1141 agaggtcgag tcggtggctg atgttgcccc cgttgccgtg gcagactgcc agggtcaact
     1201 gcgatcgcag cgagttcgaa tgcgccgcga agaaccaggc gcttaactcg atgccgtcag
     1261 cagtctgcag gcgcacgtct tcccagggac gccccagggc gcgccccgtc tcggcccatt
     1321 ccctggaagg gtggtagact tgtttgtgct cgaaccagcg caacattgcg tagagtatta
     1381 gcggcagcag gacgatcgcg gccaggatcg ttcggtgctt gcggtgcggg tgaatcgtca
     1441 cagaccatga ggggcgggcg cgcatacggc gcaataggac cgccaagaag cgcctgaggc
     1501 attcgagtcg gcaaaccgga aactgccgtc gtcgagaacg gtgtcaacct gcaacgaggt
     1561 ccaatctcag gcatcgcgca aggctaccgt gcaacggtga cacctggcaa gcctggagcg
     1621 acgtgtgccg attggccgcc acggttattt cgttcaaatt cctgaagggt tgctgttgtt
     1681 gaggttggaa agagatggag aacctgtcac tttattgctg tcgttgttcc atgcccacct
     1741 ccgggcgcaa ctccgggctt ccgccgcggg cgcggttctg ctgtgatttt caaccgtcat
     1801 ggccacgccg gatttgctga cccgcctgtt gcgggacgta tcgcgctcgt tctaccggac
     1861 gctgcgcgtt ctgccgtccg gcgtccggcc ccagatcggt cttgcgtacc tgctggcccg
     1921 aatcaccgat accatcgccg acacggagat tgtcagtctc gaaggccgga tggcggcgct
     1981 cgatcgactg cggacccgca ttgccatccc gagatcgggg tccgtccgcc tcgaatcgtt
     2041 tttcgccgac gcgggcgcgg ggcagggggc atcagccgaa cgcgatctcc tcttgcgcgc
     2101 cgacgaggcc ctgggcgtgc tggatgcagt tgaaccgggc gacgggcggc taattcggaa
     2161 cgtgctgcgc acgatcatca gcggccagga actcgatctg cgtcgatttc gcggcgccag
     2221 cgcccagtgt ccggcagcgc tcgataccga ggaggatctc gatgactaca cctatcgtgt
     2281 ggccggatgt gtcggcgaat tctggacgca tctctgccgc gcccggctct ttccggcggt
     2341 gcgggtcgac gagggattcc tcctggcgaa cggcgttcgg ttcggcaagg gccttcagct
     2401 cgtgaacatc cttcgcgaca ttccgcgtga tctgcgtcat gggcggtgtt atctcccctc
     2461 gaatcgcctc gcggcgcacg gtatgagggc ttcgtcgctg ctggatgcgc ggaacatgcc
     2521 caggtttcgc aagctctacg aggattacct cgatctggcc ggcgcctgct tggcggacgg
     2581 gtgggcttac acgaacacgc tgccttcgcg gcagatgcgc gtccgattgg cctgcgcatg
     2641 gccgattctg attggccttc ggaccatcgc ccgcctgcgc gttgaaaacg tgttggatga
     2701 tcggcgtcgc atcaaggtga gccgcggcga ggtgaaacgg ctcatgctgc gatccgtctg
     2761 cgtttacccc tgggcccgcg cctggaaccg gctgttcgag cgcgcgcgcc aaatgccatc
     2821 ctctgtttta tgactgccgc ctcagccaaa gcgcgccacg ccgatctggc cgcccaaatc
     2881 cgcgtccacg accacgcgta ctatgtcctg gccaggccca cgatcagcga ccgcgagtac
     2941 gatcggctct accacgaact gctcgaactc gaacgccaat ggccggaact cgtcacccca
     3001 gattcgccct cgcagcgcgt cggcggcgcg ccgctggccg agtttcgtcc ggtccggcat
     3061 gcgcagccga tgcagagcct ggacaacacc tattcgcagg cggaggtgaa ggagttcgtc
     3121 ggacggattc agaggctgct gccgggcgaa ccgctcgaat ggtgcgtcga gcccaagatc
     3181 gatggtgtgg ccgtgagcct gcgctacgag aacgggcgac tggccgtcgc cgccacgcgt
     3241 ggcgatggca cgactggaga cgacatcacc gcgaacctca ggacgatccg cagcgtgccg
     3301 ttggtgcttg cgcatccgcg gtccgagctc gcctcacggt cggcaccagg cgggaccgcg
     3361 aagaccgggg aggagtgccg gcgcccgacg caaggcgatc tgtttggagc gggcgctgct
     3421 ggaggtgccg ggcaggcggg gccgatcccg cgagtgctcg aagtgcgggg ggaagtgttt
     3481 ctcggcaaag ctggatttgc gcggttgaac gaagagcggg aggcgctcgg ggaagagccg
     3541 ttcgccaatc cccgcaacgc gacggcgggc tctctcaagc agctcgatcc gcgtattgtg
     3601 gcggcgcggc cgctcgacat cgtcctgtac ggcctcgggc acgtggaagg cgtccgggcg
     3661 ccggccacac agatcgagtt gctcgattgg ctcagggcgc tggggctgag gacgccccag
     3721 ccacgctggc tgtgccgttc actcgatgaa ctgttcgagg ccattgcgag cctggaaggg
     3781 cagaggcgcg actttgcgta ccaaacggac ggcgccgtca tcaagctcaa ctcggtcgcg
     3841 ttgcgggcgc ggtgcggcag cacatcgaag gcgccgcgct gggcaatcgc ctacaagtac
     3901 gccgctgaac aggctcagac gcgcctgaac gcaatcacaa tccaggtcgg acgcaccggc
     3961 gccctgacgc cggtggccga gctcgagccg gtgtttctct cgggcagcac catcagccgc
     4021 gccacgctgc acaacgaaga ggagctccga cgcaaagaca tccgtcttgg cgactgggtg
     4081 gtcatcgaga aggcgggcga ggtcattcca gccgtggtcg gagtcgtgct cgaacgccgg
     4141 aacggccatg agaaacagtt cgaattccct cggtcttgcc ccgagtgcaa atcgaaggtg
     4201 tcgcgcggtt cggtccttgg cgaggccggg gtggtctggc gctgcgtcaa tccggattgt
     4261 ccggccaaga ttcgcgggcg aatcgagcat tggtgctcgc gcggggcgat ggacatcgag
     4321 ggcggcgggg aagtgctggt ggcccaactc gtccgtaatg gcctggtccg ggatgtcgcc
     4381 gacctctatc gcttgcgcct ggatgaactc gccgccctcg aacggatggg agagaaatcc
     4441 gcccggaact tcctggctgc gatcgaggcg agcaaacggc gcgaactctc gaggctgatt
     4501 ttcggcttgg gcatcctgca tgttggcgcg cgcgtggcca aatcgctggc ccgccggttt
     4561 gcgacgctcg atgacctcat gcgggccggc caggagcatc tgacggccac ggaagacgtg
     4621 ggcgagatca tcgccgccag cgtttaccaa tggtgcagcg acgccgagaa ccggcgtttg
     4681 atcgaacgtc tgcgcaaggc gggtctgagt ttcgagtcca ccgcattcca ggccggttcc
     4741 ggacgcggcc cgtttgccgg caagtccttc gtgctcacgg ggacattgcc gacgctcacg
     4801 cgcgaacaag cgaccgccaa gatcgaggcc ctcggcggcc gcgtcagcgg cagcgtcagc
     4861 aagaaaaccg acttcgtgct cgccggagcc gatccggggt ccaaactcgc caaagcccag
     4921 aagctcggcg tgcgaatcat cgatgaagcg gagttccgcc gccttggagg cgcgggctga
     4981 ccggatgcca aagaccgatt ccatcgagtt gggcaacggc gagcgtctgc ggattctcca
     5041 cgaagaccgc gccgtgctgg cgatcgacaa acctgccggc tggatgctgg tgcccgtcag
     5101 ttggcagaac accggacgca acctccaggc ggccttgctc tcgtgcctgg cctcgggcgc
     5161 gtattgggtc cgctcgcgca acctgacgtt tctgcgctat gcgcatcgcc tcgatgccga
     5221 gacgacgggc gtgctgctgc tggcgaagag ccgcggcgct ctcgatgcgc tgggcggtct
     5281 gtttgagagc cgcctcatgc agaagtctta cctcgccgtc gtgcaggggc gcccccctcg
     5341 ggacgcatgg acctgcaacc tgcctctggc cccgcatccg cggaaacacg gacgggtcat
     5401 tgtcagccgc cacgatggca aatcgactga aacccgattc cgcgtcgtgg agcggaagac
     5461 cgacgcgcag agggggacgt ggtcgttgat cgaggcgcgt cctgtcaccg gccggacgca
     5521 tcaaatccgc gtccacctcg ccgcggatgg tttgccggtg ctgggagacg ccctctacgg
     5581 gccccgtcag tccggcttga tgccggacga ctttccgctg gctttgcggg cagtgagcct
     5641 cgcttatgcc gatccgttca cccggcgggc ggttcgaatc gatgcgcccc ggacgctctt
     5701 tcttcgcgcc ttcggcttcg agttgccggc tgccggcgaa ccccgaggga acagccgccg
     5761 cccgccacaa ccgggattgc cagccgggcg gtttgtcgac accctcggcc catgattgaa
     5821 ccgtggccca cactcgagtc taagacgctc ggcgacttcc gcgtttttcg ggtgcgacag
     5881 gattccaaac gctcgcctcg caccggtcag acgcacaact ttttcgtcct cgaatgcccc
     5941 ggctgggtca acatcattgc cctgaccccc catcgggaca tcatcctggt cgagcagtat
     6001 cgacacgggg tccagacggt cgaactcgaa gtccctggcg gcgtgatgga cgccgacgac
     6061 gaatcgccga tcgagaccgc ggtgcgcgag cttcgggagg agaccggttt cgagggcgag
     6121 aacgcggttg tcatcgggcg cgttgccccg aatccggcaa ttcagaacaa catcgccggc
     6181 acggttctca tcgagaattg cgtgcggcgg catgaggtcg agtttgacca cacggaggac
     6241 gtggtgacgc gaatcgagaa agtcgatgcc ttgcctctcc tggtggcttc cggccgcatc
     6301 cgtcattcgc tcagcatcgc cgccttttac cacttcgatc tctggctccg cggcctggcg
     6361 cccaacgcgc ctcgccacta acccaaaaag ctaaaaagga agtctccccg ctgttgtggc
     6421 cgggtgcgaa tccgctttcc aggatgcgct ttttcacgtc agcgcctccc gcacccggcc
     6481 cagttccgcc ggcgacacac actcgatctg ctcatccatc gggtaaccgg tcggcccggg
     6541 ataaaccacc cagagcttcg ccagcttcaa gtcctgcatg gcgatccgca tggatttggt
     6601 gatcgtcggc gcgtcctgat acttgatctc gaagccccag cgttggcctt tggccggcac
     6661 catcaaatcc agctccgccc ggctgtgcgt cgcccaaaaa taggcgttcc gctccccggc
     6721 ccacgccagg atttgctcga tgacgaatcc ctcccacgac gcccccagtt tgggatgccc
     6781 tgacaacgcc gcctcatccg ggatgcccag cagcgcgtgc aacaaccccg aatcccgcac
     6841 atgcaccttg ggcgacttga ccacgcgctt gcccacgttc tcgaaccacg gcgggagctg
     6901 gcgcaccatg tacgcgccgc tcatcgtgtc caaataccgc cgggccgcgt gatgcgtcac
     6961 cccgacgcgc tgcccagcag caagaaccgg caagggggcg gctgccgatc cgccagcacc
     7021 cgcaagagcg gcatcaggtc gggccgttga tgaatttcat ccagcaccac caacccccgc
     7081 gccgcttcca acaccgtttg cggatgcgcc agccgcgcca ggttgcgggg actctccaag
     7141 tcgtagtaat ttttcgatga gactccgccc aacattcggg ccagcgtcgt cttgccacat
     7201 tgacgcggcc ccaggagcaa cgtgcccggg ctgtgagcca gtccgtcgtt cactctcaca
     7261 accagttcag gacgaggaat cctccaagac ctccggctcg gcgtattcca actcgaactg
     7321 ggcccggagg atggcctcca gttcttcaca ccgtgccacg gaacgcctcc ttgaggatgg
     7381 cggcctgccg ttctggcttc aactccgtgt gccactgggt aaacgtaacg cgcagcgatt
     7441 cgaccttggc agcaagggcc ggcggcgtca gccgcaacgg tttgagggtc tcggcgtcgt
     7501 cacattcgag cagcaagccc tcgccgctgg caatggcggt ggagagcagg gcgcggtgaa
     7561 gttgcgagtc atcctcgccg ggctgggggg cgagcaacat ttgctcctcc aagtcctggc
     7621 aaacgcggca caggttgaac caggaaacga cccgggcggc aagaggattc cggcgcatct
     7681 tgacttcggc ttcgagcaca tcggcaaagc ggttcgccgt gcgccagcgt tctcttacgg
     7741 tttcgggcat tgtagtcccg ataggcataa cgggaaagta tgagcggatg tggcgtcctg
     7801 caagtcactt ttgccgagcg ttctccgcgc aagggcgcat ctcagtttct tgcaagccag
     7861 ttggaggata ttccttgtat cttgcgtagc ccatttttga cggttcattc cactatcgct
     7921 ttcaacagcg cctcgcgggc gtcgctgtag tagaggatgt ttaccttggt gatgaggtcg
     7981 tcgggcaggt cgttgagctg cttccgggcc gagaccggca tcagcagggt ggtcgccccc
     8041 ttctcgatgg ccagctccgc cacgttgacg gcgttgtgaa ccgggtctat cgagccgccg
     8101 aggttcagac cgccgataac caccagcccc cccttggtgc ttttctggag cagggcgctg
     8161 catagagcga ccaagaccgc caggcccgtg ccggtgccgt tcttgccggc atcgaaggcc
     8221 cggagctgga tcgaaaactc gtgttcgcgg ggatcgcggt cgccggtcaa ctgcttggcc
     8281 cgcgagtaaa gattctgctc ggcgtagcgc acgctctccg agaacggtgc cggcgccggc
     8341 cggttcagta tcttcacgcc cgatcccggc ccctcgttga cttcgatccg gtaaagcccc
     8401 gcgttctcgt ctagtccgcc cggactgatc gcccatatct ggccaggcgg cagcggatcg
     8461 gagccgatgc tgtcctcgct ctggagttcc ggcgtaacca cgaacttctc cacgccgtcc
     8521 tgccccaagg tataactgaa atgcgtattc ctgaactccg ctgacccgat acgcttctgc
     8581 tgctctttca cgcgacgacg gcactccagc gccagccgca ccgcccattc cagcgcctcg
     8641 tcggtgaccg ggctgtcggg gttggggaac agcagcttga gcaggccgtt gacagttctt
     8701 tggaccgccc atgtatcgcg cccgcttaac gcaccaccga agaatacccg cccctggagc
     8761 gccgtgagtc ggctttgttt acgaagatga gtccagcact ccgacaggaa gtcgctgacc
     8821 aggccgaagt ggttggtgaa aagttggccc gacatcttgg gcacgtccca gcccggcgca
     8881 tagcagtgga ttcgatcctg gaaggccgtg tcgtcgcgca tctctggcgg cagcggcccg
     8941 aacaggtgcc cgatcttctg ctggtgctgc acgtccacgt cgaagttgcc gaccatcacg
     9001 atgccgccat cggcgcggat gctttccttg ccccggctga actcgcccga ctccatgtag
     9061 cccttcatga tgttcacgcc gtccttctgg tcgaacgaca cgccggagat ttcgtcgaaa
     9121 cagaccacat catactgaca caccaagccg cgctggccgt tggccatgtt gacgaacatc
     9181 cgcgccacag tcgtcttgcc gcctgaaacc agatgcgcgt aaggcgaaat ctgctggaac
     9241 agatggcttt tgcccgtccc tcgcggcccc agctcgacca tgttgtagtt gcgctccacg
     9301 tagggcacca tccgcaggaa gaccacgtcg atgttgcgcg gcgtcagggc gtccggttcc
     9361 aggccgatgc tgcggatcag gaaccgcttc cactcttcca ggttgaacaa ctcccgtccg
     9421 cgatacatgg cttccaggac atcccgtttc gagagctgga tttcccgaat gctctccacc
     9481 ccgaaaggcc gaccggactg ctccgcggca atggtcgcgt cgtaggtcaa ttcgatctcg
     9541 gcgtaaaagc cgcccgtgag catccggtcg tgctgctgaa ccagggcggg cgtgatccgc
     9601 acgtcgcgca ggcgcaggct gggcagctcc gccacgtagg agtcggtctt ggcgtccagg
     9661 cgagccgtga tgatgtcgat gatgcggatc gagccggtct cgcgggcctt ggacttgaaa
     9721 tactcttcct cgccgggccg caccgtccgg tcggccaact gccgctgcac aacctgcaag
     9781 ccctcctcga tctctttttc gtcggtcgag gagcagtagc gtccgagcag gaactccgcg
     9841 acgtaggtgg gcaccggata ttggcccttg aacttcctga ccaaatcctt ccgcacgatg
     9901 tagccctcga aggctttggc ggcgagtttg tcgatggcat caagctccat aaatcattct
     9961 ccaatcacgg tcgggaattt ggcgactacg gtatcgccgg cgtccacgag gacgactgtg
    10021 gccttggctc ccgact
//
LOCUS       DAOQRO010000091        10009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6065_length_10009_cov_4.131304, whole genome shotgun sequence.
ACCESSION   DAOQRO010000091 DAOQRO010000000
VERSION     DAOQRO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_10009_cov_4.131304"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..2235)
                     /locus_tag="PKK20_05030"
     CDS             complement(136..2235)
                     /locus_tag="PKK20_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNU99282.1"
                     /translation="MLETLLYPRTVAVIGASRNPEKVGYAFMHNLTRGGFKGTIVPIN
                     LEASEILGVKCYKSLAEYGGKIDLSVVVVGGKYVRQAIQDSIDAGAKTVIVITAGFKE
                     VGAKGAEAEKELVALCQAHGVRMMGPNCLGVLNTHHAMNATFAPTVPPPGRISVISQS
                     GALCVAILDWAASQELGLGKVISFGNKADLNEVDFIQALAEDKETRVIAGYLESIKEG
                     DKFLRVAEQAAGVKPVVVLKVGTTQAGAKAASSHTGSLAGADIAYGAAFKRSGVIRAE
                     NFEALFDYAMAFSTQPLPAGERVCVITNAGGPGIMAADAAETMGLKMVIPSDATKERL
                     RAVLPPTASFGNPVDVIGDAEPERYLKAFEILQEDDTIDAILVVATPQNMTRPLELAE
                     KLAAANKGKKPLLTAFMGGQEVAAGKARLMELGIPNYPAPDRAVAALRAMCDYAAWRR
                     RPARIVSRFPVNHRRVDRVIQWHIRSEIEQVGEVEAKDILRAYDFKILPGALAKDADE
                     AVEVANRIGYPVVLKISSPDIIHKSDFGGVRVNLAAAEQVRDAFDLMMLRVQRRAPNA
                     FIRGADVEKMGHRGREVILGMTRDPQFGPMLMFGLGGIFVEVMKDVTFHLAPITQEEA
                     MQMLMGTRSYALLKGARGQAPVDLHALASALQRISQLATDYPEIRELDINPYIVGEVG
                     TEAYAADARMTLSKLAN"
     gene            complement(2490..3224)
                     /locus_tag="PKK20_05035"
     CDS             complement(2490..3224)
                     /locus_tag="PKK20_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNU99283.1"
                     /translation="MPYRFLTLDEAAEHLGLRRTELERLVKARLIQFETRGERTVFRR
                     QDLEDWASRHILGAAGAESSRNDEPSWPHACTLSSAAPLLPSLIRPEQIDAAMTAKTK
                     AAVLHDLVEMAKRTGWVCDPRELTSSLQAREALCSTAVPGGVAFLHPRAQDPYRFTAS
                     FLLLGRTVCPIHYGAPDGLPTELFFMLGCQEDRLHLHTLARLCLVAQKTNVLEQLRAA
                     PDSSAMHACLLAAEQAMLPTMSGVKR"
     gene            complement(3239..6805)
                     /locus_tag="PKK20_05040"
     CDS             complement(3239..6805)
                     /locus_tag="PKK20_05040"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNU99284.1"
                     /translation="MNRHNWFRLRRPLPKTAARMQRAMGYRLWIAAVWLSALVAASVW
                     TRAALVINEVCYDNSVFADENGDTSSDWIELYNSGPGSVNVSNYGLGDGNPYDETKGV
                     RLPNYVIPPGGTLVVFANSDLPEYTAWTNAPDIALIAENATWRYASGSSAPAAAWKSA
                     AFNDSAWASGMAPIGYNDRTLDLDCATVLAYGGNPDNRHPAAYFRKTFNVINPSVITG
                     LVVRARVNDGMVVYLNGSEVLRQNMPAGAVSHATLASIEVPPTWWTTNVLPTTGLSRG
                     TNALAVEVHQASVSSADLIMDLTLTALVNEQVPIVHGRFGLKKDGEHVHLFNNALTRI
                     HKLDSPGYEIGKDKSYGLVPDGFTGAMKVYEIPSPGLPNATYETKYAETLISQKPTFS
                     IPPGVYASGQNVVLRTMTAGYRVFYTLDGSDPRDSTTFVYSGNAVSVSAAAPLTSGLS
                     WIRTSPVEIANAVPGAAWQAPLGSVTRAVVLRAIAVSADNKHCSPETRGTYFIGPEFA
                     NRAMPIVSLISDSDNLFGFTSGLYVPGKHYADSPEGYGENKWGKPHANYHQESKDGSW
                     ERPLQIEFFETNQTKAAFTQLLGLAMHGGGTRAMPQKALYLLARLGKYGADRIEYRLF
                     PDEAATDYKRFLLRSSGNDWYGPDHAGVATMLKDAVFHRMVRGLDISVMAYRPTVVYI
                     NGEYWGIHNLRESFDKHYLATRYGLEADNCDILMHQEDSRDKDKVRIERIDGDRNADE
                     EYEALIGWIQANPPSQAENYAQLQTSIDVNNYTDYIIAETFFANTDWPINNCDFWRAH
                     TNQVASSGKYGDTRWRWMLYDLDVAGAKGPAFDMFGYLSSKNMTGGREPGFLINQLWS
                     NMDFRNQFVTRYANLLNTTFRPERLQRIISDAAAVIAPEIETHFRRWGRVHTQAQWLD
                     AVSQVLVRFTATRHDVSWDHLDKFFGLGGTGTLTVKNNDPSGAGGRFAVSGVVIDSTT
                     EGVTDRAEWTGRFFRSLPVPVQALPDAGYLFDGWAGTAITNPSPAVFVGQMPATLVGR
                     FRPDSAPAHVPTGYERWQVANYSEQEILGDAAAAPDAPSGFAGMSNFALYAFGMQRND
                     GMTDEERFARSSVSIRGQDDRLWLGYARLNSSFTDVRYSLKIARSLSTPIAWRDAVVG
                     VDLDTTTTTNILDANTWLVEVRLPAMSPKHDTRYFMLEAAPQ"
     gene            7047..8825
                     /locus_tag="PKK20_05045"
     CDS             7047..8825
                     /locus_tag="PKK20_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNU99285.1"
                     /translation="MFLIDTLILVTGVLLLLGLASSKLAARLGMPVLVLFLLVGMIAG
                     SEGIGGLDFEDYRLAHGIGTVALAVILFDGGLGTSLAAIRLSWKPSILLATVGVLVTA
                     VITGVAASWVLDISLMEGLLLGSIVGSTDASAVFVILRWGGVSLAKRIAAVLEVESAS
                     NDPMAIFLTIGCIEVLLGHVTIGPSLLGLFASQMAIGAIWGVAGGYATAWLVNRIELG
                     AAGMYPVLVSACCLLIFGAAAQLGGSGFLAVYLAGVVIGNRRLAFQRSIRRFHDALAW
                     MGQIVMFVTLGLLCFPSRLWDVGGKAILICIVLMCVARPVAVLISAMPFCFTERELGF
                     MSWVGLKGAVPITLATFPLMLATPENSLQAPLIFDVVFFIVVLSAIVQGTSLNPAARW
                     LGLERPRDPEPPVTLEISSLRHVDGEVVDYAIGEDSRAAGRMVKDLALPGGVVIALIA
                     RGDRVIPPQGITRIHTGDHVILVLRPGTQPLVNQVFGRISDKPAMVPKAIEFPFRGST
                     TVGEFEEFYSIHIDAPPKTTLDEAMRRELGPEQTALDAVVVFGPLRFRIQRLSETGRI
                     ELVGMAILPKSEDTESEAERSEDGIG"
     gene            9132..9206
                     /locus_tag="PKK20_05050"
     tRNA            9132..9206
                     /locus_tag="PKK20_05050"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9164..9166,aa:Lys,seq:ttt)
     gene            9331..9861
                     /locus_tag="PKK20_05055"
     CDS             9331..9861
                     /locus_tag="PKK20_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99286.1"
                     /translation="MKKTLTMAVATLIVGAVTASAAVIDVNTLVDTRDNYFATGEWHL
                     PGGAVTWAAIPGSGHFWNVDVSGNVFSLGGGVNLYAVAVKGNHKVPSPGGPTASLAVG
                     LVDYVGASGHATVTVPHPGGLTSQDLWFLNAEINPAGKMNFKVTASHPIPEPGAFALI
                     AGLGLVGFAAYRRTRA"
BASE COUNT         1799 a         3002 c         3161 g         2047 t
ORIGIN      
        1 ggttccgcgc acccggtgcc gatgaagatg cgctggccgg ggcggatttt ggccacagcc
       61 tcgtcgggcg tggcggtcat cgatttatac ttctccctcc actccggatc gtaagtgacg
      121 ttgatgttct tcatgttagt tcgcaagttt ggagagggtc atccgggcgt cggcggcata
      181 ggcctccgtg cccacctcgc ccacaatgta ggggttgatg tccagctccc tgatctcggg
      241 ataatcggtc gccagttggc tgatgcgctg gagcgcgctg gcgagcgcat ggaggtcgac
      301 gggcgcctgg ccgcgcgccc ctttgagcaa ggcatacgag cgcgttccca tgagcatctg
      361 catcgcttcc tcctgcgtga tcggcgccag gtggaacgtc acgtccttca tgacctcgac
      421 gaaaatgccg cccagaccga acatgagcat cggtccgaac tgcgggtctc ttgtcatccc
      481 aagaatcacc tcgcgcccgc ggtggcccat cttctcgacg tcggcgcccc gaatgaaggc
      541 gttgggcgcg cgcctctgga cgcgcagcat catgaggtca aaggcatcgc gcacctgctc
      601 cgccgcggcc aggttgaccc gcacgccgcc aaagtccgat ttgtgaataa tgtccgggga
      661 cgagatcttg agcacaaccg ggtagccgat tcggttggct acctcgacgg cttcgtcggc
      721 atccttcgcc aatgcgcccg gaagaatctt gaaatcgtag gcgcgcagga tgtccttggc
      781 ctcgacctcg cccacctgct cgatctcgct ccggatgtgc cattggatga cacggtcgac
      841 gcggcggtga ttgaccggga atcggctcac tatccgggcc gggcgccgtc gccaggccgc
      901 gtaatcgcac atggcccgca acgcggccac tgcgcggtcg ggcgccgggt agttgggaat
      961 gcccagttcc atgaggcgcg ccttgcccgc tgccacctcc tggccgccca tgaacgcggt
     1021 gagcaacggc ttttttccct tgttggccgc agcaagcttc tcagccaact cgagaggccg
     1081 tgtcatgttc tgcggcgtgg cgacgaccag gatcgcgtcg atcgtgtcgt cctcctgcaa
     1141 gatttcgaag gccttgaggt accgctccgg ttcggcatcg ccaatcacat ccacagggtt
     1201 gccaaacgag gcggtcgggg gcagcaccgc gcgcagccgc tccttggtcg catcgctcgg
     1261 aatcaccatc ttgagcccca tcgtctcggc agcatcggcc gccatgatgc ccggaccgcc
     1321 ggcgttggta atgacacaga cccgctcgcc ggcgggaagc ggttgggtgg aaaaggccat
     1381 ggcgtaatcg aacaacgcct cgaaattctc cgcccgaatg acgcccgatc gcttgaaggc
     1441 cgcgccgtac gcaatgtcgg ctccggcgag actgccggta tgggacgagg ccgccttggc
     1501 ccccgcctgc gtggtcccga ccttgagaac cacgaccggc ttgacacccg ccgcctgctc
     1561 ggcaacgcgc agaaacttgt ccccctcctt aatgctctcg aggtaacccg cgatcacccg
     1621 cgtctccttg tcctcggcca gcgcctggat gaagtcgact tcgttcaggt ccgccttgtt
     1681 tccgaagctg atgaccttgc cgagacccag ctcctggctc gccgcccaat cgaggatggc
     1741 tacgcacaag gcgccggact gcgagataac cgaaatgcgg ccgggcggcg gcaccgtcgg
     1801 cgcaaaggtg gcgttcatgg cgtggtgcgt gttgagcacg cccagacagt tgggccccat
     1861 catccgcacg ccgtgcgcct gacaaagcgc gaccaattcc ttttcggcct cggcgccctt
     1921 ggcgcccacc tccttgaaac cggcggtaat gacaatcacg gtcttggcgc cggcatcgat
     1981 cgagtcctgg atggcctgtc tgacgtactt gccccccacc accacgacgc tcaagtcgat
     2041 cttgcctccg tactccgcga ggctcttgta gcacttgacg ccgaggatct ccgaggcttc
     2101 caggttgatc gggactatcg tgcccttgaa cccgccacgg gtcaggttgt gcatgaaggc
     2161 gtagccgacc ttctccgggt tccgtgacgc gcctatgacc gccacggtcc gtgggtaaag
     2221 caatgtctca agcatactcg taatctttca aggccgcctt gcgaagcagc aacccgctct
     2281 tgggccgaat tctctgcatc ccgcgtgccg ccagacattt gccccggaat gcaagggaat
     2341 acgcaccccg gcagttccaa cggcaattcg gccaccgcgg tgaggctaga gattcccgcg
     2401 ccgattcact cgtccggaat tggcgaaagc gcgccatttt ttggagaaaa ggggtcggtc
     2461 cgggaattga tgaacaggcg atgacaaaac taccgtttga cccctgacat cgttggcagc
     2521 atcgcctgtt ccgcagccag caggcacgca tgcattgcgg aggaatccgg agcggcgcgc
     2581 aactgctcga gcacgttggt cttctgggcc accagacaca gacgcgccag cgtgtgaagg
     2641 tgcaaccggt cctcctgaca gccgagcatg aaaaacaact cggtgggcag gccgtcgggg
     2701 gcgccgtagt ggatcggaca gaccgtgcgg cccagaagca gaaaggacgc ggtgaatcgg
     2761 tacggatctt gagctcgcgg gtggaggaac gccacgccgc ccggcacggc cgtcgagcaa
     2821 agggcctctc gagcctggag gctcgaggtc agttcccgcg gatcgcaaac ccatccggtg
     2881 cgcttggcca tctcgaccag atcgtgcagc acggcggctt tggtctttgc ggtcatggcc
     2941 gcgtcaatct gctcgggccg aatcaggctg ggcaacaacg gggcagcgga tgacagcgtg
     3001 cacgcgtgcg gccatgatgg ctcgtcgttc cgggaggact cggcccccgc ggctccgagg
     3061 atgtgtcgcg acgcccaatc ctcgaggtcc tgtctgcgga acaccgtgcg ctcgccacgg
     3121 gtctcgaact ggatgagccg ggccttgacg agcctctcga gttcagtgcg tcgaagaccg
     3181 aggtgctccg ccgcttcgtc gagagtgaga aaccgatacg gcatgcgtgt tgttccgttt
     3241 actggggtgc ggcttcgagc atgaaatagc gggtgtcatg cttcggcgac attgcgggga
     3301 gacggacctc gaccagccat gtgttcgcgt cgaggatatt ggtcgttgtc gtcgtatcga
     3361 gatcgacgcc gacgaccgcg tccctccatg cgatcggggt gctgagactg cgagcgatct
     3421 tcagcgaata tcggacatcg gtgaagctgc tgttcagacg ggcatagccg agccagagcc
     3481 ggtcgtcctg gccgcggatc gacaccgagg atcgggcgaa gcgctcttca tcggtcattc
     3541 cgtcattgcg ctgcatgccg aaggcgtaga gcgcgaagtt gctcatgccc gcgaatccgg
     3601 atggagcatc cggtgcggcg gccgcatcac ccaggatctc ctgttcgctg taattcgcca
     3661 cctgccaccg ctcgtagcct gtcggcacgt gagccggggc ggagtcgggg cggaatcggc
     3721 ccaccagcgt cgcgggcatc tggccaacga acacagcagg gctgggattc gtgatcgccg
     3781 tgcccgccca accgtcgaac agatagccgg cgtcgggcag ggcctgcacc gggacaggca
     3841 agctgcggaa gaacctgcca gtccactctg cgcggtcggt cacaccttcg gttgtcgagt
     3901 caataacgac gccgctgacg gcaaaccgcc ccccagctcc gctgggatcg ttgttcttca
     3961 ccgtcagtgt cccggttcct ccgaggccga agaatttgtc cagatggtcc cacgagacgt
     4021 cgtggcgggt ggcggtaaac cggaccagga cctggctcac cgcatcgagc cactgcgcct
     4081 gcgtgtggac gcggccccag cggcggaagt gtgtctcgat ttcgggagcg atgacggcgg
     4141 cggcatcgga gataatccgt tggaggcgct ccgggcggaa ggtggtgttg agcaagttgg
     4201 cgtagcgcgt cacgaactgg ttcctgaaat ccatgttcga ccagagctgg ttgatcagga
     4261 aacccggttc cctgccgcct gtcatgttct tggacgagag gtagccgaac atgtcgaaag
     4321 cggggccctt ggcgccggcg acatccaggt catagagcat ccagcgccag cgtgtgtcgc
     4381 cgtacttgcc ggaggaggcg acctggttgg tgtgggctct ccagaaatcg caattattga
     4441 tgggccaatc ggtgttggcg aagaatgtct cggcgatgat gtaatccgtg tagttgttga
     4501 catcgatcga ggtctggagt tgggcgtagt tttcggcttg gctcggcggg tttgcctgaa
     4561 tccagccgat gagcgcctca tactcttcat cggcgttcct gtcgccgtcg atccgctcga
     4621 tgcggacctt atccttgtcg cgcgaatcct cttggtgcat gagaatgtcg cagttgtcgg
     4681 cttcgagtcc gtagcgggtg gcgaggtaat gcttgtcgaa gctctcgcga aggttatgga
     4741 tgccccagta ctcgccgttg atgtaaacca cggtcggacg ataggccatc accgagatgt
     4801 ccaaacctcg gaccattctg tgaaacacgg cgtccttgag cattgtggcg accccggcat
     4861 ggtctggccc ataccagtcg ttgccgctgc tgcgcagcag gaagcgtttg tagtcggtcg
     4921 cggcttcgtc cggaaaaagc cgatactcga tccggtctgc gccgtatttg cccaggcgcg
     4981 ccagcagata aagggccttt tgcggcatcg cccgtgtccc gccgccgtgc atggccaagc
     5041 ccaatagctg agtgaacgca gcctttgtct gattggtctc gaagaactcg atctgcagcg
     5101 gacgctccca actgccgtcc ttgctctcct gatgataatt ggcgtgaggc ttgccccatt
     5161 tgttctcccc gtacccttcg ggggaatcgg cgtaatgctt ccccggcaca tagaggccgg
     5221 aggtgaaccc gaagagattg tccgaatcgg agatcaggga gacgattggc attgcgcggt
     5281 tcgcgaactc cggtccgatg aaataggtgc cgcgtgtctc gggggagcag tgtttgttgt
     5341 cggccgagac ggcgatggcc cgcaacacca ccgccctggt cacgctgccg agcggggcct
     5401 gccatgcggc gcccgggacg gcgttggcga tctcgaccgg actggtgcga atccacgaca
     5461 gtccggaggt caagggagcc gcggcgctga ccgacacggc attgccgctg tagacgaatg
     5521 tcgtcgagtc ccgcgggtcg ctcccatcca gcgtgtagaa caccctgtaa cccgcggtca
     5581 ttgtcctgag caccacgttt tgcccggatg cgtagacgcc cggcgggatc gaaaaggtcg
     5641 gcttttgcga aatcagcgtc tcggcgtact tcgtctcata cgtggcattg ggcagaccgg
     5701 gagagggaat ctcgtacacc ttcatggcgc cggtgaaacc gtccggcaca agtccgtagg
     5761 acttgtcttt gccgatctcg taacctggag aatcgagttt gtggatgcgg gtgagagcat
     5821 tgttgaacag gtgcacgtgc tcgccgtcct ttttcaagcc gaaacggcca tgaacgatgg
     5881 gcacctgctc gttgaccagc gccgtgaggg tcaaatccat gatcaggtcg gcactcgaca
     5941 ctgaggcctg atgcacctcg acagccagcg cgttggtgcc gcgggacaaa ccggttgtgg
     6001 gcaaaacgtt tgtggtccac caggtgggcg ggacctcaat cgaggcgagc gtggcgtggc
     6061 tgacggcgcc ggcgggcata ttctggcgca agacctcact gccgttcaga tagacgacca
     6121 tgccatcatt gacacgggct ctgaccacga ggccggtgat cacggacggg ttgatgacat
     6181 tgaatgtctt ccggaagtaa gccgccgggt ggcggttgtc cggattgcct ccgtaagcca
     6241 ggaccgtcgc gcagtccagg tcgagcgtgc ggtcgttgta cccgataggc gccatgcctg
     6301 aggcccaggc gctgtcgttg aaggcggcgg acttccatgc ggcggccgga gcgctcgaac
     6361 ccgaggcata cctccaggtc gcattctccg caatcaacgc aatgtccggg gcgttggtcc
     6421 acgccgtgta ttcgggcaga tccgagttgg cgaagaccac aagtgtgcct ccaggcggaa
     6481 tcacatagtt gggcaaccgg acgcctttgg tctcgtcgta aggattgccg tctcccaatc
     6541 cgtaattcga cacgttgaca ctgccgggcc cggagttgta gagctcgatc caatctgaac
     6601 tcgtgtcgcc gttctcgtcg gcgaaaaccg agttgtcgta gcagacctcg ttgatcacaa
     6661 gggcagcgcg cgtccagacg gacgccgcca caagcgcgct caaccagacg gcggcgatcc
     6721 acagtcgata ccccatcgca cgctgcattc gggcggctgt cttcggcagc ggacggcgga
     6781 gacgaaacca attgtgacga ttcatagtgc cacctcaagc aattcgcatg cccgtctgaa
     6841 tccgggcgct aaacctcgca agggtatcaa tgaccaggcg agggcgcaag cgccctaaaa
     6901 gcgcgccgaa tgaccattgt cagacgcgag gccgcgatgt agcctgccgg gcctgaagag
     6961 cgtcggcaga gggtggctgg ccaaaggacc gatgcgcatc atcgccggga gtgtctgctg
     7021 cggacaacga tggggacccc agtccgatgt ttctgatcga cacgttgata ctggtgacgg
     7081 gtgtcttgct gctgctggga cttgcttcga gcaaactcgc ggcgcggctg ggcatgccgg
     7141 tgctcgtcct gttcctcttg gtgggcatga tcgcggggtc cgaaggcatc ggaggtctcg
     7201 atttcgagga ttaccgcctg gctcatggta tcggcacggt ggccctggcg gtgatcctct
     7261 tcgacggcgg acttggcact tcactggcgg cgatccgtct gtcgtggaag ccgtcgattc
     7321 tgttggccac ggttggtgtg ctggtgaccg cggtcatcac cggtgtggcg gcatcctggg
     7381 ttctcgacat ttcgctgatg gagggtctct tgttggggag cattgtgggt tcaacggacg
     7441 cctcggccgt atttgtgatt ctgcgctggg gcggcgtgag tttggccaag cgcatcgcgg
     7501 cagtgctcga agtcgagagc gcatccaacg accccatggc gatctttctg acgatcgggt
     7561 gcatcgaggt tctgctcggg cacgtgacca ttggaccgag cctgttgggt ttgttcgctt
     7621 cgcagatggc gatcggggca atttggggag tcgctggagg ctatgccacc gcttggctgg
     7681 tcaatcgcat cgagttgggg gccgccggca tgtaccccgt ccttgtcagc gcctgttgcc
     7741 ttttgatctt cggggcggcc gcccagttgg gtggcagcgg ttttctcgcc gtgtatctgg
     7801 ccggggtggt catcggcaac cgtcgactgg ccttccagcg gagcatccgc aggttccacg
     7861 atgccctcgc gtggatgggc cagattgtga tgttcgtcac gctgggtctg ctctgttttc
     7921 cgagtcgatt gtgggacgtg ggcggcaaag ccatcctgat ctgcatcgtg ctgatgtgcg
     7981 tcgcacgccc ggtcgccgtg ctgatctctg ccatgccctt ttgtttcacc gaacgcgaac
     8041 tcgggttcat gtcgtgggtg gggctcaagg gagctgtgcc gattacgctg gcgacgtttc
     8101 ctctgatgct ggccacaccc gagaactctt tgcaggcgcc gctgatcttc gatgtggtct
     8161 tcttcatcgt ggtgctctcg gccatcgtcc agggaaccag tctaaatccg gcagcccgat
     8221 ggctcgggct cgaacgcccc cgagacccgg aacccccagt tacgctcgag atcagctctc
     8281 tccggcatgt ggacggcgaa gtggtcgatt atgcaatcgg cgaagactcc cgggcggcgg
     8341 ggcgcatggt gaaggacctg gcactgccag gaggcgtcgt catcgcgttg attgcgcgcg
     8401 gcgaccgggt cattcctccg cagggcatca cccgcatcca taccggcgat cacgtcattc
     8461 tcgtgctgcg accgggcacc caaccgctgg tgaaccaggt cttcgggcgc atcagcgaca
     8521 agccagccat ggtgcccaag gccatcgagt tccctttccg cggctcaacc accgtgggcg
     8581 agttcgagga gttctattcg attcacatcg atgccccgcc gaaaaccact ctggacgagg
     8641 cgatgcgacg cgagcttgga ccggagcaaa ccgcgttgga cgccgttgtc gtgttcggtc
     8701 ccctcagatt ccgcatccag cgtctctcgg aaaccggcag gatcgagttg gtgggtatgg
     8761 ccatcttgcc gaagtcggaa gacaccgagt cggaagcgga acgttccgaa gacggaatcg
     8821 gttgagagcg ggataaagcg attctcctaa tctccaatag cggccgaaac tgcctcgccg
     8881 aaaactctcg gaaaactgct tgcccccacg agcggctgag gcatagtacc ggcccgttga
     8941 cttgatgcga tgcagacacc cgccgccttc attcaccaag ccgttcttcg ggtcttgttc
     9001 gatttacggc ttgggctgcg gagcatgagc aagagaaggt ttgcggcaac gcgacgtgga
     9061 ttgtctgtcc aagggctgca tcgagtcttt ttgattgagt tgtcttaagt tgctttttag
     9121 cagtatgtta tgggttggta gctcagcggt agagcagcgg ccttttaagc cgttggtcgt
     9181 gggttcgagt cccacccgac ccaccaattc ccaagcgtaa aattttccag aatttacttg
     9241 acgagatttg gctatgtgat ataaacgccg cacgacttgg tcgctccagg tcgtgtttcg
     9301 gccgaatcaa cagtaaaaag aaaggtaaag atgaagaaaa cactcacaat ggctgtcgcc
     9361 acgctcatcg tgggagcagt cacggcgagc gcagcggtta tcgatgtaaa cacactggtg
     9421 gacacccggg acaactactt tgcaacgggt gagtggcatc tgccaggcgg ggcagtaacg
     9481 tgggcagcca ttcccggcag tggccacttc tggaacgtgg atgtctccgg caacgtcttc
     9541 tccctcggtg gtggggtgaa tctgtacgcc gtcgctgtca agggtaacca taaggtcccg
     9601 agtccgggag ggcctactgc atccttggcc gttgggcttg tcgactatgt cggcgcctct
     9661 ggtcatgcca cggtcaccgt tcctcatcca ggcggtttga ctagtcagga cctttggttc
     9721 ctgaacgcag agattaaccc ggctggcaag atgaacttca aagtcactgc ctctcatcca
     9781 atccctgaac cgggtgcatt cgcgctgatt gcgggtcttg gcttggttgg gtttgcggct
     9841 tatcgccgca cgcgggccta agcctgacga caggcaattc actcattcgc aggcgccgag
     9901 caatcggcgc cttttttgtt tttttgtctc gtcagcagaa tctcaggaac gccttggccc
     9961 aggtcaggga gcgatagtgc cagaagtcct cgaaggtctc cttgagcat
//
LOCUS       DAOQRO010000092         9990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6083_length_9990_cov_5.039356, whole genome shotgun sequence.
ACCESSION   DAOQRO010000092 DAOQRO010000000
VERSION     DAOQRO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_9990_cov_5.039356"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1052
                     /locus_tag="PKK20_05060"
     CDS             138..1052
                     /locus_tag="PKK20_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNU99287.1"
                     /translation="MLSAFIAACLFSASIIAADRSARLLGSHAANFWRVVVAVLLLGF
                     WAHGFGRGLGGGVFLLFFLSGCIGFGFGDMALFLALLRLGPRLSALMINCLAAPFAAA
                     IEWAWLGTRLSLVQSLCGVLILGGVALALLPERRQTTPRGARAAGIGFGIIAALGQGG
                     GAVLSRKAYAVAADMGEQIDGGTAAYQRILGGLMIVLIPMLWAMLHQSLKPRPATPGC
                     RPPGTRAAATKWVIINGLAGPTVGVAAYQWALKTTPAGIVLPITATTPLLVIPFTYWF
                     EGDRPGVNSLTGAVAAVLGVIGLTLTTG"
     gene            complement(1042..1521)
                     /locus_tag="PKK20_05065"
     CDS             complement(1042..1521)
                     /locus_tag="PKK20_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNU99288.1"
                     /translation="MDALLKLLRDNAALEPAQLASMLNLTEAEVAERIRGYEQDRVIL
                     AYQAVLNEEKLGADGVRAVIEVKITPEREGGFDRIAERIAKYSEVRSCYLMSGAYDLL
                     VIVDGDSLRDVAMFVAQRLATIHGVLSTATHFMLRPYKEQGVLMRPEAGEERLAVSP"
     gene            1753..2604
                     /locus_tag="PKK20_05070"
     CDS             1753..2604
                     /locus_tag="PKK20_05070"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HNU99289.1"
                     /translation="MHSDYQLVRLANGAFSIRDRAAGETFHPVIGPRAEAEALYVRPL
                     RIRERLQSESGEFVIWDVGLGGAANIATVLRSVQDLPGRLRVVSFDRTLEGLRFALNH
                     AHDLEYFAGWEDVCSALLEHRAISLARSALKLDWDRHLGDFPVLLGRPEAAAWPKPHG
                     ILFDAFSPARNPDMWTAPLFARLHEILDPRRPCMLATYSRSTMLRVTLLLAGFHVGIG
                     GATGEKEETTIAANTGDLIEHPLDRRWLGRARRSRSAEPLRTPHYRQAPLSTESWDRL
                     LTHPQFR"
     gene            complement(2652..3467)
                     /locus_tag="PKK20_05075"
     CDS             complement(2652..3467)
                     /locus_tag="PKK20_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNU99290.1"
                     /translation="MRAQDVNATMAVFLDLENIALGARDAHHPRFDIRKVLERLLLKG
                     HIVVKKAYCDFDRYKEFKRDLHEAAFELIEIPHVRQSGKNSADIRMVVDALDLCYTKG
                     HVDTFVILSGDSDFSPLVSKLRENAKTVIGMGVKSSSSDLFINNCDEFLYYDDLVRAE
                     SVKARQRSRVPSTPAKTARTSDAGGGPPPDDAFKHLMTTFDAVLEEREEDEPVWGSMI
                     KQAIKRRNPGFNERAHGFRSFNDLLREAEKRGLLRLEPDEKSGGYIVREVEEV"
     gene            complement(3519..6638)
                     /locus_tag="PKK20_05080"
     CDS             complement(3519..6638)
                     /locus_tag="PKK20_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNU99291.1"
                     /translation="MDTRLRKARGMASLGRWLALALASAWPLAADVLFEDDFDRSFPG
                     WTAVRPAGAYLDGPLRWQYDIVTGAFFEQSNIYTDSSSHSPTATAPMLINDAVTAVNF
                     TYTARLTAGDDDGFGLVFGYQDDQNFYRVTFARQLRTAGFPWTGWSVDRKVNGVTFNL
                     FGAGTPGYTPTFVNTAGQPFDVTVSVSTANRLTLTVVDNPTGVLRVYPLVADQPLPSA
                     ANGKVGLFTWGMSGGTPNGFRIQDLRLASVGLAGDPNALSEWTPVVPPRANGSRALTG
                     GSGQAVWSLSLGEAGSYGVLTESGDAFGGNDVAGQVDFTGPVLVAGDAGWTNYTVALR
                     VTPWDDDGHGLLVRYRDETNFYRIALRSQVSTVGVPPGLSVQKCVDRAYTEVYRDNPV
                     RYNPVAGQTYDLVATIGGDRLDVAVIADPEGAADVYTYEPMTLSGVSNGKIGLLSWAM
                     SRTEFDWITVQDGATLYVSSPYGAPSPPRGLNSSSPGAVVDARAGITLEAAGLRRVPV
                     GWTGSGSVPARGSGSNVVFTLDDPSRLHWIWQTECRLSLVAGPGGAVSHPPISWFPQG
                     ADVFVSAEPRDGYLFAGWTGDSVSGSPALHVTMDRPRELVAEFTPDTDRDGLADDWER
                     IYFGDLQAMPEEDADGDGRTNREEYVNGTHPKVPDILRITGLELGGGRGILSISNNTG
                     TRYGVQVAADLAHWSTVATAQFAARFTSSVPAGAQAFWRLQQPGGSPGALPFVPGSWT
                     LAVLPDTQIYAMRYPDLFMDQTRWIAANRDRYSIEYVLHLGDITNNNTSNQWQNAKSA
                     LSLLDGVVPYALVPGNHDYGANGGTENRGTQLHTFFPVADCAAWPTFGGVMEPGRLDN
                     SYHLFRAGGVDWLLLALEFGPRDSVVAWAGQIAAQHPSRKKILITHAYTYSDDTRYDW
                     AGKGTAQSWNPHGYPIGSSPDGTNDGEELWRKLVKVYPGFVLVLSGHVLNDGLGRLST
                     ANDFGEVVHQMLVNYQMQAQGGEAVLRLIEFLPDGRTVRVKAYSPLSGVHKTDPQNQF
                     VLTLDPPLR"
     gene            6818..8152
                     /locus_tag="PKK20_05085"
     CDS             6818..8152
                     /locus_tag="PKK20_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99292.1"
                     /translation="MKINLCRSKSSRLLSIAVVLGALAAGASALAAPPPGFTALFNGR
                     DLSGWRGGDTFDHRKLLAMPAEQRAAQIAKWNESMKAHWRAENGLLVNDGHGAYATTE
                     KDYGDFELLVDYKTVPKADSGIYLRGVPQVQIWDSTEEAKFSIGANKGSGGLWNNSPG
                     APGKDPLILADKPFGEWNSLRIMMVGSRVSVWLNDKLVVDHAVMENYYDRKVCVPARG
                     PIQLQTHGGEISWRNIYLREIGAAEANQILARKGNDGFETIFNGRDFSGWAGAVANYE
                     VKDGAMACRPGKGGTVYTEQQYGDFAVRLEFKLPKGGNNGLAIRYPGNGDAAYAGMTE
                     IQVLDDNYEQATGHTIDPRQAHGSVYGMIAAQRGYQRPIGEWNFQEVTVQGSRVRVEL
                     NGFVILDGDVATVKEFMGNTPHPGKDRTKGHFGFAGHNDPVCFRNVQLKPLD"
     gene            complement(8179..8781)
                     /locus_tag="PKK20_05090"
     CDS             complement(8179..8781)
                     /locus_tag="PKK20_05090"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNU99293.1"
                     /translation="MDHDPLILASASPRREDLLRGLRIDFAVCPARVAEVRQKQLTAR
                     EMSMLNAFRKARDVARQFPDQRVLGADTLVSLGRRLYGKPKDLGEAARMLRELAGRTH
                     QVVTGVCLMHWRARRQRLFAEVTNITFLPLTPEQIEAYLAAVNPLDKAGAYAIQQRGS
                     DLVAGIVGSYSNVVGLPLERLQEELRLLNGPHPADRRSAE"
     gene            complement(8825..9898)
                     /locus_tag="PKK20_05095"
     CDS             complement(8825..9898)
                     /locus_tag="PKK20_05095"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HNU99294.1"
                     /translation="MSSHSEHHPHRRRNPLTGQWVLVSPHRTLRPWHGHREHTADQRR
                     PQYESKCHLCPGNVRANGVHNPQYTHTHVFTNDFAALLPETPLGETPGDDLLMRVESV
                     RGTCRVICFSPRHDLTLPEMEQADIERVVDVWADQTSELLREYRWVQIFENKGAMMGC
                     SNPHPHGQVWAGSSLPNEARLEDASQRAYFQTQDTPLLIDYAKRERQRGERVVVSSDH
                     WLAVVPFWALWPYETLVVPRRHVRRLPELDASERADLAGVLKRLLTRYDNLFETSFPY
                     SMGWHGAPGGEDDATHWQLHAHFYPPLLRSAHIKKFMVGYEMLAEAQRDITPEQAARR
                     LQEMPECHYLARRASDQPQSIVQ"
BASE COUNT         1864 a         3266 c         3088 g         1772 t
ORIGIN      
        1 aatccacgta aaggccgctc aaataaatga cttacaccgg ctcagacccg gtcggaaaag
       61 tgcagccaat gggggaagac cagccggacg gacagcgcgc atgccgcagg cgttcccgct
      121 gactccccac tccatccatg ctctccgcct tcatcgcggc atgcctgttc tcggcatcga
      181 tcatcgccgc cgaccgttcg gcccgtctcc tgggaagtca tgcagccaat ttctggcgtg
      241 tcgtggtggc ggttctcctg ctgggattct gggcgcacgg attcggtcga gggctcggcg
      301 gcggggtctt tctgctcttc tttctgagcg ggtgcattgg atttggcttt ggcgacatgg
      361 ccttgtttct ggccctgctc cgcctcggac cccgtctcag cgccctgatg atcaattgcc
      421 tcgccgcgcc attcgcggcc gcgatcgagt gggcctggct cggcacgcgt ctttccctcg
      481 ttcagagtct gtgtggagtg ctgattctgg gcggcgtggc gctcgctttg ttgccggaac
      541 gccgccagac cacaccgcgt ggcgctcgag cggcggggat cggattcgga atcatcgccg
      601 ccctgggaca gggcggaggc gcggtgctga gccgcaaggc ctacgccgtt gccgcggata
      661 tgggcgaaca gatcgatgga ggcaccgccg cgtaccagcg catcctgggc ggcctgatga
      721 ttgtcttgat cccgatgctc tgggcgatgc tgcatcagag tctcaagcct cgtcctgcca
      781 cgccaggctg ccgtcccccg ggcaccagag cggccgccac caaatgggtc ataataaacg
      841 gcctggccgg tcccaccgtc ggagtggcgg cctatcaatg ggcgctcaag accacaccgg
      901 ccgggatcgt tcttcccatc acagcgacca cacccttgct ggtcattccg ttcacctact
      961 ggttcgaagg cgaccgcccc ggagtcaact ctctgacggg cgccgtcgcc gccgtgctgg
     1021 gcgtgatcgg gttgactttg actacgggct gaccgccagt cgctcttcgc cggcttccgg
     1081 acgcatcagc accccctgct ccttgtaagg cctgagcatg aaatgcgtcg cggtcgaaag
     1141 cacaccgtga atagtggcca accgctgcgc gacgaacatc gccacgtccc gcaacgaatc
     1201 gccgtcgact atcaccagca aatcgtacgc ccccgacatc aggtaacatg accgaacttc
     1261 ggagtatttc gcaatccgct ccgcaatgcg atcgaagccg ccctcgcgtt ccggcgtgat
     1321 tttgacctcg ataacggcac gaaccccgtc tgcgcccaac ttctcctcgt tgagcacggc
     1381 ctggtaggcg agaatcaccc ggtcctgctc atatccccga atccgctcgg caacctcggc
     1441 ctccgtaagg ttcagcatcg aggccaattg agcgggttcg agcgcagcgt tgtcgcggag
     1501 caatttcagc aaggcatcca tgacctgagg tttaccctcc tcgagcccgg gcgcaaagct
     1561 aaaacagacc actcactagt catcgagtct tgtccacccc gaacccgaag actccagaga
     1621 gaccctatgc gggcccggtt gcggttcctc gcaaagcatg tgaaatcacc tgcctaaacg
     1681 accggttccg gcgtggcggt cgaacacgga ctcgccccgg cgcccgggat gcgatgtaat
     1741 gcccgtgtga cgatgcattc ggactatcag cttgtgcggc tcgcaaacgg ggcgttcagc
     1801 atccgcgacc gcgcggcggg ggagactttt cacccggtga tcggaccgcg agccgaagcc
     1861 gaggccctgt atgtgcgtcc tttgcgaatc agggaacggc tgcaaagcga gtccggcgaa
     1921 ttcgtgattt gggatgtcgg cctgggaggc gcggccaaca tcgcaacggt tttgcgctcg
     1981 gtgcaggatc tgccgggccg gcttcgcgtt gtcagtttcg accgcacgct cgaaggattg
     2041 cggttcgcct tgaatcacgc gcacgacctt gagtacttcg ccggctggga agacgtctgc
     2101 tccgctctcc tcgaacaccg tgcgatctcc ctcgcgcgct ccgccctcaa actcgattgg
     2161 gatcggcacc tgggagattt ccctgtcctg ctcgggcgcc ccgaggccgc ggcctggccg
     2221 aagccgcatg ggattctctt cgacgccttc tcgccggcca ggaacccgga catgtggacg
     2281 gcgccgctct ttgcaaggct gcacgagatt ctcgatccgc ggcggccctg catgctggcg
     2341 acctattcgc gcagcaccat gctgcgcgtg acactgctgc tggccggctt tcacgtcggc
     2401 atcggaggcg cgacgggcga gaaggaggag acaacaatcg cggccaacac aggcgacctg
     2461 atcgagcatc cgttggaccg gcgctggctg gggcgcgcgc ggcgctcgcg cagtgccgaa
     2521 ccgctcagaa caccgcatta ccgccaggct cccctcagca ccgagtcctg ggatcgactg
     2581 ctgacgcacc cgcaattccg atagtcgcgg tcggtcgggt gaatacctcg acggtctgag
     2641 gtggagcctc gctagacctc ctccacctcc cgaacaatat agccgcccga tttctcgtcc
     2701 ggctccaacc tcaacagccc gcgcttctcc gcctcgcgca ggagatcgtt gaaactgcgg
     2761 aacccgtggg cccgttcgtt gaagcccgga ttcctgcgtt tgatggcctg cttgatcatc
     2821 gagccccaga ccggctcatc ctcctcccgc tcctcgagca cggcgtcgaa cgtcgtcatc
     2881 aaatgcttga acgcgtcgtc gggcggagga cctccgccgg cgtcggaagt ccgcgcggtc
     2941 ttggcgggcg tcgacggcac gcggctccgc tggcgggctt tgaccgactc ggcacggacg
     3001 agatcgtcgt agtaaaggaa ctcgtcgcag ttgttgatga agaggtccga gctcgaactc
     3061 ttgacaccca ttccgatcac ggtcttggcg ttctcgcgca acttgctgac cagcggcgag
     3121 aagtccgaat cgccgctcag gatcacgaac gtgtcgacgt ggcctttggt gtaacagagg
     3181 tcgagcgcat ccaccaccat ccggatgtcg gcggaattct tgccggactg gcgcacgtgg
     3241 ggaatctcga tcagttcgaa ggcggcctcg tgcaaatccc tcttgaactc cttgtagcgg
     3301 tcgaagtcgc agtacgcctt cttgaccaca atatgcccct tgagaagcag acgctcgaga
     3361 accttgcgga tatcgaagcg gggatggtgg gcgtcccgcg ctcccaacgc gatgttttcc
     3421 agatccaaaa agaccgccat tgtggcgttc acatcttgtg ctctcatgtc gtactccccg
     3481 acaccctatc gcaccgccgg ccattgtgca cgcacgattc accgcaatgg cggatcgagc
     3541 gtcaaaacga actggttctg cgggtctgtc ttgtgcacgc cggagagagg cgagtaggcc
     3601 ttcacccgaa cagtccgtcc gtcgggcaag aactcgatca gccgcagcac cgcttcgcct
     3661 ccctgggcct gcatctgata attgacgagc atctgatgca cgacttcgcc gaaatcgttg
     3721 gcggtcgaga gccggcccag cccgtcgttc agcacgtggc cgctcaagac cagaacgaag
     3781 cccggataga ccttcaccag cttgcgccaa agctcctctc cgtcgttcgt gccgtccgga
     3841 gagctgccga tcggataccc atgaggattc cagctctgcg cggtcccctt gcccgcccaa
     3901 tcgtagcggg tgtcgtcgct gtacgtgtag gcgtgcgtga tcaggatctt cttgcgcgaa
     3961 gggtgctggg cggcgatctg gccggcccag gctacaacac tgtcgcgcgg accgaactcg
     4021 agggcgagca acagccagtc gacgccgccc gcgcggaaca gatgatagct gttgtcgagc
     4081 cggcccggtt ccatcacccc gccgaaggtc ggccaggcgg cgcaatccgc cacgggaaag
     4141 aaagtgtgca attgcgtgcc gcgattctcg gttccgccgt tggcgccgta atcgtgattg
     4201 cccggcacca gcgcataggg cacgacacca tcgagcagcg agagagccga tttggcgttc
     4261 tgccactggt tgctggtgtt gttgtttgta atgtcgccca agtgcagaac atactcgata
     4321 ctgtagcgat cccgattcgc ggcaatccat cgggtctggt ccatgaagag atcgggatag
     4381 cgcatggcgt agatctgcgt gtccggcaga accgccaagg tccacgagcc gggcacgaaa
     4441 ggcagtgccc ccggagaccc gcccggctgt tgcaaacgcc aaaacgcctg ggcccccgcc
     4501 ggcaccgagg atgtgaagcg cgcggcgaac tgcgccgtcg caacggtgga ccaatgggcg
     4561 aggtcggcgg ccacctgcac gccatagcgt gtgccggtgt tgttcgatat cgagaggatg
     4621 ccacgcccgc cgcccaactc gagcccggtg attcgcaaga tgtccggcac cttcgggtgc
     4681 gtgccattga catactcctc gcggttggtc cggccgtcgc cgtctgcatc ctcctcaggc
     4741 attgcctgga gatcgccaaa ataaatccgc tcccagtcgt ccgccagccc gtcgcgatcg
     4801 gtgtcgggag tgaactccgc aacaagttcc cgcggccgat ccatcgtcac atgcagcgcg
     4861 ggcgaacctg agacggaatc gcccgtccac ccggcaaata ggtagccgtc ccgcggctca
     4921 gccgaaacga acacgtccgc gccctgcgga aaccacgaga tgggcggatg tgacacagcg
     4981 cctcccggac cggcaacaag cgagagccgg cactccgttt gccaaatcca gtgcaatcgc
     5041 gacggatcat cgagcgtgaa tacgacgttc gacccgctgc cgcgcgcggg cacagagcca
     5101 gagccggtcc agccgacggg cacgcggcgc aggcccgccg cctcgagagt gatccccgcc
     5161 cgggcatcga ccacagcgcc cggagacgag ctgttcagac cccgcggagg gcttggcgcc
     5221 ccgtaagggg aggagacata cagcgtcgcc ccatcctgaa cggtgatcca gtcgaactcc
     5281 gttcgggaca tcgcccaact gagcagcccg atctttccat tgctgactcc gctcagagtc
     5341 atcggctcgt acgtgtagac gtcagccgca ccctccggat cggcgatcac cgcaacatcg
     5401 agtctgtccc cgccgatggt ggccacaagg tcgtaggtct ggcccgccac ggggttgtac
     5461 cggaccggat tgtcgcggta gacctcggtg taagcccgat ccacgcactt ctgcacggag
     5521 agaccggggg ggacgccgac cgtcgagacc tgcgagcgca acgcgatgcg ataaaagttc
     5581 gtctcgtccc ggtaacgcac aagcaggcca tgcccgtcgt cgtcccacgg cgtgactcgg
     5641 agagcgacgg tgtagttcgt ccagcccgca tcgccggcaa ccaagaccgg gccggtgaag
     5701 tccacctggc cggcgacatc gttgccgcca aacgcatcgc cgctttccgt cagcacacca
     5761 tacgatccgg cctctcccaa cgagagcgac cacactgcct gcccgctccc gcccgtcaac
     5821 gccctgctgc cgttggcgcg cggcggaacc accggagtcc actcggacag ggcgttcggg
     5881 tcgccggcca ggccgacgga tgcgagacgc aagtcctgaa tgcgaaatcc gttgggggtg
     5941 ccgccgctca tgccccacgt gaacaggccc accttgccgt tggccgctga gggcaacggt
     6001 tgatcggcca ccaggggata gacccgcagc acgccggttg ggttgtccac aacggtcagg
     6061 gtgaggcgat tcgccgtgct gactgacact gtgacgtcga acggctgccc cgccgtgttg
     6121 acgaaagtcg gcgtatagcc cggtgtgccc gcgccgaaca ggttgaatgt gacgccgttc
     6181 acctttcgat ccacactcca gcccgtccat gggaaacccg cggtccgcaa ctgccgggcg
     6241 aaggtgacgc gatagaagtt ctggtcgtcc tggtaaccaa acacgagacc gaacccatca
     6301 tcatctccgg cggtcaggcg ggcggtgtag gtgaaattca ccgcggtgac ggcatcgttt
     6361 atgagcatcg gcgccgtggc ggtcggggaa tgggacgaac tgtcggtgta gatgttgctc
     6421 tgctcgaaga acgccccggt cacgatgtcg tattgccagc gaagcggccc atccagatag
     6481 gccccggcag gccgcaccgc ggtccagccc gggaagctgc gatcgaaatc gtcttcgaat
     6541 aggacgtccg cggccagcgg ccaagcgctc gccagcgcga gagcgagcca tctccccaaa
     6601 gaggccatgc cgcgcgcttt gcgcagtctc gtgtccaaat gaaagggcga gcccgcgtgt
     6661 ggatgactgc tgcttaaaag aacgatcgag tgcattgtga cgttcggttg cagtgcgatt
     6721 ctgcgatgag aatcgcgtgt ctgcccacaa aaggcaatgc atcgcgcttc gcggattgac
     6781 ctttccgagg cgaggcgcta acaagcagcc cgagtttatg aagatcaacc tctgtcgttc
     6841 gaaatcgagt cggctcctct ccattgcggt tgtccttggt gcgctcgcgg cgggcgcttc
     6901 ggccctggcc gcgccccctc ccggttttac agccctgttc aacggccgcg acctgtcggg
     6961 gtggcggggt ggagacacat tcgatcaccg caagttgctg gcgatgcccg cggaacagcg
     7021 ggcggcccag atcgccaagt ggaacgagtc catgaaagcc cactggcggg ccgagaacgg
     7081 gctgttggtc aatgacggac acggagcgta cgccaccacc gagaaggact acggcgattt
     7141 tgaactgctc gtcgattaca agaccgtgcc caaggccgat tcgggcatct atttgcgcgg
     7201 cgtgccgcag gtccagattt gggactcgac cgaggaggcg aagttcagca tcggcgccaa
     7261 caagggatcg ggcggcctct ggaacaacag tccgggcgcc ccgggcaaag acccgctgat
     7321 tctggcggac aagccgttcg gcgaatggaa ttcgctgcgg atcatgatgg tgggttcgcg
     7381 ggtcagcgtc tggctcaacg acaagttggt ggtcgatcac gctgtgatgg agaactacta
     7441 cgaccgcaag gtctgcgtgc cggcccgagg ccccatccaa ctccagaccc acggcggaga
     7501 gatctcatgg cgaaacatct acctgcgcga gatcggcgcc gccgaagcca accagatttt
     7561 ggcgcgcaaa ggcaacgacg gtttcgagac aatcttcaac gggcgcgact tcagcggctg
     7621 ggccggggct gttgcaaact acgaggtcaa ggacggcgcc atggcctgca gacctggcaa
     7681 gggcggaacg gtctacaccg agcagcagta cggggacttc gcggtgcgcc tcgagttcaa
     7741 gctccccaag ggaggcaaca acggcctcgc catccggtac ccgggcaacg gggacgccgc
     7801 ctacgctggc atgaccgaga tccaggtgct cgacgacaat tacgagcagg cgacgggcca
     7861 cacgatcgac ccgcgccagg cgcacgggtc ggtctacgga atgatcgccg ctcagcgcgg
     7921 ttaccagcgt ccgatcggcg aatggaattt ccaggaagtg accgtccaag gctcgcgcgt
     7981 gcgggtcgaa ctcaatggtt tcgtgatcct ggacggcgac gtggcgacgg tgaaggagtt
     8041 catgggcaac acgccgcatc cgggcaagga tcgcaccaag gggcacttcg gtttcgccgg
     8101 ccacaacgac cccgtctgct tccgcaacgt gcagttgaaa ccgctcgact gaggcgcgat
     8161 ccgcctcctc gacacaattc actccgccga ccgtctgtcg gcggggtgcg ggccattcaa
     8221 gaggcgcagt tcctcctgca aacgctcgag gggcagcccc acgacgttcg aatacgatcc
     8281 cacgatacca gccaccaggt cgctcccgcg ttgctggatg gcataggcgc ccgccttgtc
     8341 gaggggattc accgccgcca ggtaggcctc gatttgctcc ggcgtcaaag gcaaaaacgt
     8401 gatgttcgtc acctccgcaa aaaggcgctg cctgcgagcc cgccagtgca tgaggcaaac
     8461 ccccgtgacc acctggtgcg tccgacccgc caactcccgc agcatccgag ccgcctcccc
     8521 aaggtctttc ggcttgccat acagacgccg cccaagactc accagcgtgt ccgcccccag
     8581 cacgcgctga tcgggaaact gcctggcgac atcacgcgcc ttgcgaaatg cattcagcat
     8641 gctcatttcc cgcgcggtga gctgcttctg gcgcacctct gcgacgcggg ccgggcatac
     8701 cgcaaaatcg atccgcagcc cgcgaagcaa gtcctcgcga cgcggcgacg ccgaggccag
     8761 gattagtgga tcatggtcca tagattgcgt tcacattcaa cggctcaatg cgcacgggga
     8821 cttttcattg gactatggac tgtggctggt ctgaggcgcg cctcgccagg taatggcatt
     8881 cgggcatttc ctgcaatcgt ctggcggcct gctcgggggt gatgtcacgc tgagcttccg
     8941 ccagcatctc gtagccaacc atgaacttct tgatgtgcgc cgatcgcaat agcggaggat
     9001 agaaatgggc gtgaagctgc caatgggtgg cgtcgtcctc gccgccgggc gcgccgtgcc
     9061 atcccatcga gtagggaaat gaggtctcga acaaattgtc gtagcgcgtc aggagacgct
     9121 tgagaacacc cgccagatcc gcccgttcgc tcgcatccag ttcaggcagg cggcggacgt
     9181 ggcgtcgcgg cacaacaagg gtctcgtagg gccacaacgc ccagaacggc accacagcca
     9241 gccaatggtc ggaagacaca acgacccgct ccccgcgctg gcgctcgcgc ttggcataat
     9301 cgatgagcag cggcgtatcc tgcgtctgaa aataggcgcg ctgcgaagcg tcttcgagcc
     9361 gcgcctcgtt cggcaggctt gacccggccc aaacctgacc gtgggggtgc ggattcgagc
     9421 agcccatcat ggcgcccttg ttctcgaaga tctggaccca gcggtattcc ctgaggagtt
     9481 ccgatgtctg atccgcccac acatccacca cccgctcgat gtcggcctgc tccatctcgg
     9541 gcagcgtcaa atcgtggcgc ggagagaagc agatcacacg gcacgtcccc cggaccgatt
     9601 caacgcgcat caggagatca tcgcccggcg tctcccccag cggggtctcc gggagcagcg
     9661 ccgcaaagtc gttggtgaac acgtgcgtgt gggtgtactg gggattatgc acgccgttgg
     9721 cccggacgtt gccgggacag aggtggcact tggactcgta ctgcggtcgc cgctggtccg
     9781 ccgtgtgctc gcgatgtccg tgccacggac gcagcgtccg gtggggcgac acgagcaccc
     9841 attggccggt cagcgggttg cgtcgtcgat gcggatgatg ttcggaatga gaactcacag
     9901 cggcgagtct cctagcccgg atgtttcaca aagcctcgcg gagtctgaca tccgggctct
     9961 ggcaaaaccg cgcccagaaa gggaaccggg
//
LOCUS       DAOQRO010000093         9989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6086_length_9989_cov_5.972317, whole genome shotgun sequence.
ACCESSION   DAOQRO010000093 DAOQRO010000000
VERSION     DAOQRO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_9989_cov_5.972317"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..567)
                     /locus_tag="PKK20_05100"
     CDS             complement(163..567)
                     /locus_tag="PKK20_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99295.1"
                     /translation="MSEIADYVIHGARLITPAIVENVLRRLPLWKAEFTQIAAPGYPH
                     LVDQLEFLANAIEDFAEGVYKDLPYSALAAAVFALTYAHKKVDIIPDYLHEAGRADDS
                     SIVRAVLIMHEKAFVKYAAAQGIEWGRITSKP"
     gene            complement(694..1038)
                     /locus_tag="PKK20_05105"
     CDS             complement(694..1038)
                     /locus_tag="PKK20_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99296.1"
                     /translation="MHLRTLGDLKGKTVALQQCGPRVGMLDDVLKSAQLTWNDIRVVW
                     AKDLSASPDSPPELFRKRADIDACFAITPDMVGLCGGLTRVGTGAETTVKRARVLVST
                     AQLSRSIADVYE"
     gene            1080..1466
                     /locus_tag="PKK20_05110"
     CDS             1080..1466
                     /locus_tag="PKK20_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99297.1"
                     /translation="MKTNLSALIAGLCLIALTVGCYHSVDGRNRAGTPFVKDRIESRY
                     ERPLDQIFEAAREVLAFNGTLTGENRISNSLEARIDNSKVWVKLDEVEPSICRVMVQA
                     RKSGGGGNVALASEIDKQIALQLQAR"
     gene            complement(1488..2732)
                     /locus_tag="PKK20_05115"
     CDS             complement(1488..2732)
                     /locus_tag="PKK20_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99298.1"
                     /translation="MTDFLHIAGRILGALGGLVLAVWILMVWWKKSDDRPGLFMRWML
                     TLADLLFLGLVVGPLVGRFDYGAAFVGVPMAAVGGFILAIIWVPHLAGAVGRKFGQLY
                     DGGDVPPDPEPFFSIAEARQKTGRYIEAVAELEKQLEVFPTHFRGLMMLAEIQADNLH
                     DLPAATETIERIASQAVHAPKNVAYAFTRLADWQLKYLKDPVAARETFQRIVDLFPDS
                     PEAYHAHQRLAHLATAEFLAGATERKPLKLTRHEDRLGLRPDFEGLKPPAPDPVGRVE
                     VLVRQLEQFPLDSQAREELALVYACDFGRLDLAAEQLEQLIAQPCAPEAQITRWLNLL
                     ADLQAREGGDVALARQTLERIIERSPESGAAEAARRRMATIGLELRAKKESRAIALGS
                     YEKRLGLKMGGPPPRQTPPPSP"
     gene            complement(2789..4048)
                     /locus_tag="PKK20_05120"
     CDS             complement(2789..4048)
                     /locus_tag="PKK20_05120"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU99299.1"
                     /translation="MSRFGNLEFSRSRIEQALRSGEVSDEAVHLREADAAFRRGAFEQ
                     ALRFYARALERAPNDAAAWVGQARMLIEMGQFDEANRWADKALERFPRHADALAAKAV
                     VLARRGDTKAALAFSDAALEEESSTPYAWLARGDVLLARAEKRSEHCFAKALAVASGD
                     WVWPWLASRIHAFYRKFSLTMQRLDALLQAAVERGASDLHLVAGAPPAFRINGEIILA
                     DGDILTSTELEDLAGCMLNAEQAAAFRRKWELCTSLHCPEVGRVRVALYRRDGHAEFS
                     FRFCGEEIPSREDLGLPSKLDELARRPNGLLLIAGPTGSGKTTTLNYIVDLINSERRC
                     NIVTIEDPIEFVHRNKRAIVVQQECCRPDARPASGPHRPADRLARIFHTPMVHGALRL
                     RKRTAMRAREAKPRQVRPIENRITLFP"
     gene            4642..5427
                     /locus_tag="PKK20_05125"
     CDS             4642..5427
                     /locus_tag="PKK20_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNU99300.1"
                     /translation="MKRSIVCLLAGAGFVFCLPSPGGEPTSAKEFLPPTPEGKEWKLV
                     WQDEFNGATLDETKWNRLGDWKRRDGFWVKDDAYVDGQGHLVLRTKKDGERFTCGAVN
                     TRGKFEHAFGYLVARCQMPKQPGHWPAFWLMCDGVNTTGDEGRDGTEIDIVEVPWRDG
                     QLTSNLHWDGYGKEHKSAGIKFKVDGVMEGWHTFGLLWLPEEYVFYVDGKETWRTTAG
                     GVSRVPEFIKLTEEIGTWGGDIKKAVLPDYFRVDYVRVYSIVP"
     gene            5434..5976
                     /locus_tag="PKK20_05130"
     CDS             5434..5976
                     /locus_tag="PKK20_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNU99301.1"
                     /translation="MNAPARSPSLELILGRRSIRVYRPDEIDEPTVRALLEAAMAAPS
                     AGAKDPWRFVVIRSRSTLEAMTRILPNGQMLATAAVGIVVCGELEAAHDRQLSYLLQD
                     CAASVENLLLAAHGLGLGACWLGIHPREARVQKIASLLEMPEGVIPVAAVALGWPGET
                     KEARTRFNPARVHRERWGSP"
     gene            complement(6112..7524)
                     /gene="glnA"
                     /locus_tag="PKK20_05135"
     CDS             complement(6112..7524)
                     /gene="glnA"
                     /locus_tag="PKK20_05135"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNU99302.1"
                     /translation="MSTPKQVIEMATKAGAKMIDFKFVDTFGTWQHFSCPIGELTEDI
                     FTEGLGFDGSSIRGWKSIEASDMLAMPDPATAFMDPFCAVPTLSLTNTIAETGTKEPY
                     SRDPRGIAQKGEKYLASTGLADTADFGPEAEFFIFDNVQFDAKSNGTFYTVDSEEASW
                     NTARDEGPNLGYKIRHKEGYFPVAPADTQQDIRTEMCLLMQELGLNVERQHHEVATAG
                     QAEIDLRFDTLVKTADNMMLFKYVIKNVARRHGKTATFMPKPLFGDNGSGMHTHVSLW
                     KKGKPLFAGQEYAGLSRMALYFIGGILQHAKALSALCSPTTNSYKRLVPGYEAPVNLA
                     YSARNRSAAIRIPTFSDSPKAKRIEYRPPDPSANPYLCYTAILMAGLDGVLNKVDPGE
                     PMDKNLYELAPEELVKVPQVPGSLPEALDALGRDHEFLLKGDVFTRDFLETWISAKRK
                     EYDALRLRPHPYEFFLYYDV"
     gene            complement(7895..8206)
                     /locus_tag="PKK20_05140"
                     /pseudo
     CDS             complement(7895..8206)
                     /locus_tag="PKK20_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
     gene            8283..9407
                     /locus_tag="PKK20_05145"
     CDS             8283..9407
                     /locus_tag="PKK20_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNU99303.1"
                     /translation="MHRTGAAASRRLMSVDALRGFDMFWILGADGLVRGLDGIRQSGL
                     TGFLGRQLTHKDWDGFAFYDLIFPLFVFIVGMSLVFSLSRTIAEQGHTAALRRVLRRS
                     ALLFALGIFYSGGFSNEWPDIRLLGVLQRLALCYLFAGLAFCFLHTRGLVVLCASLLL
                     GYWALMTFVPVPGVGAGSFAKGANLANAIDAQWLPGKKYDGSWDPEGLLSTLPAIGTC
                     LLGVFAGLWLKRDDVPAGRKVGLLLLAGASSVGLGFLWGLQFPVIKKIWTSSFVLVAG
                     GYSALLLAVFYQVVEVWRWQRWAQPFVWIGLNPITAYLANNIIGFRRLATRLAGGDVK
                     NFFNAVIAPGFGELMISIVGLGLAFLLCWFLHRRRIYLRL"
     gene            complement(9458..>9989)
                     /locus_tag="PKK20_05150"
     CDS             complement(9458..>9989)
                     /locus_tag="PKK20_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99304.1"
                     /translation="ARICPAAPSAPTKAKRRHPSSGSLNQTWLWTGSKGIDYEGSYAF
                     NGWFYGDDDPFFSTVEYKPKKYRNETDCPSPSTTPVIADSNWIDTWPQPNDPPARNLY
                     TGDDYAGAMVRLTLPRHGGAPPWPKNQQFNPKNDLPGAINISYVDGHADSVKLERLWS
                     LDWHKGWVTPARRPGK"
BASE COUNT         1780 a         3190 c         3114 g         1905 t
ORIGIN      
        1 cttccgcaga ccgcaattgc cgaccgtacc cttccgatcc gattcccagc cgcgagagaa
       61 cacaaggaac ataaagaagt gaacgtgtcg gctttgcgtt cttttgcggc tgaagacgga
      121 cggcgaaaca tgttgcagac aagtcgagct gcggcacaca ccctaaggct tgctggtgat
      181 gcgcccccac tcgatgccct gtgcggcggc gtacttgacg aaggccttct catgcatgat
      241 cagaaccgcg cgcacgatac tcgagtcgtc tgcgcgccct gcctcgtgga gatagtcggg
      301 aatgatgtcc accttcttgt gcgcgtacgt gagtgcgaac accgcggcag ccagagcgct
      361 gtacggcaga tccttgtaaa cgccctcggc aaaatcctcg atcgcgttgg cgaggaactc
      421 aagttgatcc accagatgcg gatacccggg agcagcaatc tgcgtgaact cggctttcca
      481 aagcggcagc ctccgcagca cattctcgac aatcgccgga gtgatcagcc gcgccccgtg
      541 aatcacatag tctgcaatct cagacatgcg cccgagccta gcagcgcttc ctgccaagta
      601 aatcgcaaag aaggcgtggc cccgcggacc tccacggttt cgtgagcaca atcggcgcag
      661 tcacgagaga aatctccgcc gatcggcttc tcatcattca tatacgtccg caatcgagcg
      721 cgaaagctgc gcggtcgaga ccaggacacg cgccctcttc accgtcgttt ccgcgccggt
      781 gccgactctc gtgaggccgc cgcagaggcc caccatgtcg ggggtgatcg cgaaacaggc
      841 gtcaatgtcg gcgcgcttgc ggaacaactc gggcggcgag tccggcgagg cggacaggtc
      901 tttggcccag accacccgga tgtcgttcca ggtcagttgg gcggatttga ggacgtcgtc
      961 gagcatgccg acgcgcgggc cgcactgctg gagcgcaacg gtcttgcctt tcaagtcgcc
     1021 gagggtccgc aaatgcaaaa gacttgacgg tccggcccga tgtgattgca ttcgggcgca
     1081 tgaagacgaa cctttcggcc ctgatcgccg gtttgtgcct gatcgccctg acggtcggtt
     1141 gttatcactc ggtggacggg cgcaaccggg ccggcacgcc gtttgtcaaa gaccggatcg
     1201 aaagccgcta cgagcggccg cttgaccaaa tcttcgaggc ggcccgggag gtgctggcat
     1261 tcaacggcac gctcaccggc gagaacagga tctcgaacag cctcgaagcc agaatcgaca
     1321 atagcaaggt ctgggtgaag ctcgacgagg tcgagcccag catctgccgc gtcatggttc
     1381 aggcgcgcaa gagcggcgga ggcggcaacg tcgccctggc cagcgagatc gacaagcaga
     1441 tcgctctgca gctccaggcc cggtagaggc gcgggcatcc gtcttcctca tggtgacggg
     1501 ggcggagtct gccgtggggg cgggcctccc atcttcagcc ccagccgttt ttcgtacgag
     1561 ccgagcgcaa tcgctcggct ctcttttttc gcgcgcagtt cgaggccaat cgtcgccatc
     1621 cgccgccgcg ccgcctccgc cgccccgctc tcgggggagc gctcgatgat ccgctcgagc
     1681 gtctgccggg ccagggcgac atcgccccct tcgcgggcct gcaaatccgc cagcaggttg
     1741 agccagcggg tgatctgcgc ctccggcgcg catggctgag cgatcaactg ttcgagttgc
     1801 tcggcggcca gatcgagccg cccgaaatcg cacgcataga ccaacgccag ttcctcgcgc
     1861 gcctgactat cgagcgggaa ttgctccaat tgccgcacca gcacctccac ccggccaacg
     1921 ggatcgggcg ccggtggttt gagtccctcg aagtccggac gcaacccgag acgatcctca
     1981 tgccgggtga gtttcagcgg tttgcgctcg gtggcgccgg ccaggaactc cgccgtcgcc
     2041 aggtgggcca gccgctggtg cgcatgatag gcctccggag agtcgggaaa caaatcgaca
     2101 atccgctgga acgtctcacg cgctgcaacc gggtctttca gatacttgag ctgccagtcc
     2161 gccagccgcg tgaaggcata ggccacgttc ttgggggcgt gcaccgcctg gctcgcaatc
     2221 cgctcaatgg tctccgtcgc agccgggaga tcgtggagat tgtccgcctg aatctccgcc
     2281 agcatcatca ggccgcggaa atgcgtcgga aacacttcga gctgtttctc gagttccgcc
     2341 accgcctcga tgtaccggcc ggtcttctgg cgcgcctcgg caatcgaaaa gaacggctca
     2401 ggatcgggcg gcacatcacc gccatcataa agctggccga atttgcgccc gaccgccccc
     2461 gcaagatgcg ggacccagat aatggccagg atgaaaccgc ccacagcggc catcgggacc
     2521 ccgacgaagg ccgcgccgta atcgaaccgt cccacaagcg ggccgaccac cagccccaga
     2581 aacaacaggt cggccagcgt cagcatccag cgcatgaaaa ggccggggcg gtcgtcgctc
     2641 ttcttccacc agaccatcag gatccagacc gccagaacca gcccgcccaa cgcgccgaga
     2701 atccgccctg cgatgtgcaa aaaatcagtc atgatcaagc gattgcaggc ttgacctatt
     2761 cacggccgtc ggttctgaga atggtctctc atgggaacag tgtgatgcgg ttctcgatcg
     2821 gtcgaacctg gcgcggtttt gcttctcgag cccgcatagc agtcctcttg cgaagcctga
     2881 gtgccccgtg caccatcggg gtgtgaaata tgcgggctag ccggtccgct gggcgatgcg
     2941 gtccggatgc cgggcgcgcg tcagggcgac agcattcctg ctggaccacg atcgcgcgct
     3001 tgttccggtg cacgaactcg atcggatctt caatggtcac gatgttgcag cgccgctcgc
     3061 tgttgatcag atcgacgatg tagttgaggg ttgtggtctt gccggagcca gtggggccgg
     3121 cgatcaacaa gagcccgttg ggacgccgcg ccaattcatc gagctttgaa ggcaacccga
     3181 gatcctcccg cgacggaatc tcctcgccgc agaacctgaa actgaactcc gcatggccgt
     3241 cgcgccgata gagcgccacg cgcacccgcc ccacctcggg gcagtgcagg gacgtgcaga
     3301 gctcccattt gcgacggaat gccgccgcct gttcggcgtt caacatgcat cctgccaaat
     3361 cctcgagttc ggtcgatgtg agaatatccc cgtcggcgag gatgatctcc ccgttgatcc
     3421 ggaaggccgg aggagcgccg gcgacgaggt ggagatcgga ggccccgcgc tcgaccgccg
     3481 cctgcaggag tgcatcaagc cgttgcatcg tcagcgaaaa cttgcggtag aaagcgtgaa
     3541 tgcgcgaggc aagccagggc cagacccagt cccccgatgc cacagccaga gccttggcaa
     3601 aacagtgctc cgaccgcttc tccgcccgcg ccagcagcac atcgccgcgc gccagccacg
     3661 cgtacggcgt cgaactctcc tcctcgagcg ccgcgtccga aaatgccaat gcggctttcg
     3721 tgtcgcctcg cctggccagc acaaccgcct tggccgcgag ggcgtccgca tgccgaggaa
     3781 accgttccag cgccttgtcc gcccagcgat tggcctcgtc gaactgcccc atctcgatca
     3841 acatccgcgc ctgccccacc cacgccgctg cgtcgttggg cgcccgttcg agcgcccgcg
     3901 cgtagaaccg caacgcctgc tcgaacgccc cgcgccgaaa ggccgcgtcc gcctcgcgaa
     3961 gatgcacagc ctcatcgctc acttctccgc tccgcaacgc ctgctcgatc ctcgaccggc
     4021 tgaattccag atttccgaaa cgactcatgc tgccatgact cccgcccgac gccgtcccaa
     4081 ccgccccgac aggcctcccc atgccggggt cggcgggccg gtctgcgccg ggctcgaccg
     4141 cagtgtcgca cgccggagac gcgagacaaa ttttttcacc ggccgcactg tgttggacag
     4201 tccgtgcggg tgaacgtcac gcgccgatcg aatgcgcccg gcggccagcg ccgaacaggc
     4261 gccgatgcct gacaagagcc gacgattgaa gaccttgggc tccggcaacg tccactttag
     4321 ggtctgtgca aaaattactt tcgattttgg cgggagcgcc accgggccag atgcaaggcc
     4381 cgagggaggg agtgtatccc ctgagatact cgaccgaccg aggaacgacg cagatggccc
     4441 ggtgccgccc ctccccgaag ggctgggggg ctttggctcg atggtcacgc ggacgcggga
     4501 ttgctcctcg ctcgcagacc gctgcgggta tgctccctcg tcgcgcctcg ccggcgagcc
     4561 aaatcccctc cagcaaaacc ggaattgatt tttgcacaga ccattagact gaaaccgaat
     4621 acaacgctcg gccaaatgcc tatgaaacgc tcgattgttt gcctgctcgc aggcgctgga
     4681 tttgtgttct gtctgcccag ccccgggggc gagccgacat ccgccaagga gttcctcccg
     4741 cccaccccgg agggcaaaga gtggaagctg gtgtggcagg atgaattcaa cggggcgacc
     4801 ctggacgaga ccaagtggaa tcggttggga gactggaaac gccgcgacgg cttctgggtc
     4861 aaggacgatg cgtacgtcga tggccagggc catctggtgc ttcgcaccaa aaaggacggc
     4921 gagcggttca cctgcggcgc cgtcaacacc cgcggcaaat tcgagcatgc gttcggctac
     4981 ctggtcgcgc gctgccagat gcccaaacaa cccggccatt ggcccgcctt ctggctgatg
     5041 tgcgatggtg tcaacacgac cggcgacgag gggcgggacg gcacggagat cgacatcgtc
     5101 gaggtcccgt ggcgcgacgg ccagttgacg tcgaacctgc actgggacgg ctacggcaaa
     5161 gagcacaaat cggccggaat caagttcaag gtcgatggcg tcatggaagg gtggcacacc
     5221 ttcggactcc tgtggctgcc ggaggagtat gtgttctatg tcgacggcaa ggagacctgg
     5281 cgcaccacgg ctgggggcgt ctcccgggtg ccggagttca tcaagctgac cgaagagatc
     5341 gggacctggg gcggcgacat caagaaggcc gttctgcccg actatttccg cgtcgattac
     5401 gtccgcgttt acagcattgt gccgtaggat cccatgaacg cgcccgctcg cagcccctcc
     5461 ctcgaactca ttctcggacg ccgcagcatc cgggtctacc ggcccgacga gatcgacgag
     5521 ccgacagttc gtgcgcttct cgaagccgcc atggcggcac cctcggccgg tgccaaggat
     5581 ccctggcgtt tcgtggtgat tcggtcccgc tcgaccctcg aagcgatgac gcgcattctg
     5641 ccgaacggcc agatgctggc gacggcggcg gtcggcatcg tcgtctgcgg cgaactcgaa
     5701 gcggcccacg accggcaact cagctacctg ctccaggatt gcgccgcaag cgtcgaaaac
     5761 ctcctgctcg ccgcgcacgg actggggttg ggcgcgtgct ggctgggaat ccacccgcgg
     5821 gaagcccggg ttcagaagat cgcctccctg ctcgagatgc ccgagggcgt catccccgtc
     5881 gctgccgtcg cgctcggctg gcccggcgaa accaaggaag cccgcacccg tttcaaccct
     5941 gcccgggtgc atcgcgagcg gtggggctcg ccttgatccg catccttcac agcgcagcag
     6001 ggctgcgtcc aaacgtcgta gggcagcccg cagcggcggg agaagccctc cgaagaaggt
     6061 cggctcatgc acaaatccgg tgtgtgcgcc tcaccgtggg tgcgcccggc gtcagacgtc
     6121 gtaatagagg aagaactcgt acggatgcgg gcgcaggcgc agggcatcat actccttgcg
     6181 cttggcgctg atccatgtct cgagaaaatc ccgcgtgaac acgtcgccct tcaagaggaa
     6241 ctcgtgatcg cgtccgaggg catcgagcgc ctcaggcagg cttccgggca cctgcggaac
     6301 cttgaccagc tcttccggcg ccagttcgta gaggttcttg tccattggct cgcccggatc
     6361 caccttgttg agaacaccgt ctaaacccgc catcaggatg gccgtgtagc agaggtaggg
     6421 gttggcgctc gggtccgggg ggcgatactc gatgcgtttg gccttcgggc tgtcgctgaa
     6481 ggtcggaatc cggatggccg ccgagcggtt gcgggccgag taggccaggt tgaccggcgc
     6541 ctcgtatccc ggcaccaggc gcttgtagct gttggttgtg ggactgcaca aggcgctgag
     6601 cgctttggcg tgctggagaa tgccgccgat gaagtagagc gccatccgcg aaaggccggc
     6661 atattcctga cccgcgaaca acggcttccc cttcttccag agactgacgt gcgtgtgcat
     6721 gcccgagccg ttgtcgccga acagaggctt gggcatgaac gtagcggtct tgccgtgccg
     6781 gcgcgcgacg ttcttgatca catacttgaa aagcatcatg ttgtcggccg tcttcaccag
     6841 cgtgtcgaac cgtaagtcga tttccgcctg gcccgcggta gccacctcgt gatgctggcg
     6901 ctcgacgttg aggcccagct cctgcatgag aagacacatc tccgtgcgga tatcctgttg
     6961 ggtgtcggcc ggggcgaccg ggaagtaacc ctctttgtgg cgaatcttgt agcccaggtt
     7021 gggcccctcg tcacgggcgg tgttccaact cgcctcctca ctgtccaccg tgtagaacgt
     7081 gccgttgctc ttcgcgtcga actgcacatt gtcgaagatg aagaactcgg cctccggacc
     7141 gaagtccgcc gtgtccgcca ggccggtcga cgcgaggtat ttctctccct tctgggcgat
     7201 gccgcgggga tcgcggctgt agggttcctt cgtgccggtc tcggcaatcg tgttggtcag
     7261 ggagagggtg ggcaccgcgc agaaggggtc catgaatgcc gtggcagggt ccggcatggc
     7321 cagcatgtcg gacgcctcga tcgacttcca gccgcggatc gaggacccgt cgaaccccag
     7381 accctcggtg aagatgtctt cggtgagttc tccgatcggg caggagaagt gctgccaagt
     7441 gccaaaggtg tcgacgaact tgaagtcgat catcttggcc ccggccttgg ttgccatttc
     7501 aataacctgt ttgggtgtgc tcatctcaat gtgatgctcg ttttgatgtt ctgaccgttg
     7561 gtgtcgctgt gacagtctcc gacaatctgg cgtgctccat ttcttgatcc tcgaaccagg
     7621 cgcgcttttg aattcccggc ctcaccgcgg atcaggattc catcaaacac gcgggctagc
     7681 ccgcatattt cacaccccga tggtgcacag ggcactcaga cttcgcaaga agactgctat
     7741 gcgggctcga gaagcaaaac cgcgccaggt tcgagcgatc gagaactgca tcacactgtt
     7801 cccatgagag accattctca ggaccgacgg aatgctccag gttccctttc cggaagtctc
     7861 ttcatagtcg ccacgttgtg aaacagattg gtccctaaac tggattttcc ccggtctcgg
     7921 cagtgcggat tcggacggcc tgctcgatga tcgaaacgaa gactttccca tcgtcgatct
     7981 ttccggtgcg cgcggtcttg agaaacacct gcaccgcaac gtcggcctat tcgtccgaca
     8041 aaaccaaatc cagcttgaac ttggggagga aatcgaccgt gtactcactg ccccgggaga
     8101 tctcggtgtg gcttctctgg cgaccgaatc ccttgatttc tctcaccgtc atcccctcga
     8161 tcccaatctc gcggagggca tccttcacct ctcggccgcg ggtcatcgtg aggtttgcgc
     8221 ttgttggagg gtcgcagatg acggcagact cccttccgaa ttctatgtct agttcagagg
     8281 cggtgcatcg gaccggggcc gcggcgtcgc ggcggttgat gtctgtcgat gccctgcggg
     8341 gctttgacat gttctggatt ctcggggccg acggcctcgt ccgcggcctg gacgggattc
     8401 gccaatccgg tctgacaggc tttctcggga ggcaactgac gcacaaagat tgggacgggt
     8461 tcgccttcta tgacctgatc ttcccgctgt ttgtgttcat cgtcgggatg tccctggtct
     8521 tttcgttgag ccggaccatc gccgagcagg ggcacacggc cgcgctcagg cgggtcctgc
     8581 gccgcagcgc gcttctcttc gctctgggca tcttctactc gggcggcttc agcaatgaat
     8641 ggccggacat ccggttgctg ggtgtcctgc agcggttggc gttgtgctat ctgttcgccg
     8701 gcctcgcgtt ttgttttctg cacacccgcg gccttgtcgt gctatgcgcc agtcttctgc
     8761 tcgggtattg ggcgctgatg acctttgtgc ccgtgcccgg tgtgggggct gggagcttcg
     8821 ccaaaggggc aaacctcgcc aacgcgatcg atgcccagtg gctgcccggc aagaagtacg
     8881 atgggagctg ggaccccgaa ggtctgctca gcacgctgcc ggcgatcggc acctgcctgc
     8941 tcggcgtgtt cgctggactt tggctgaagc gcgacgatgt gcccgcgggg cgcaaagtgg
     9001 gtttgctgct gctggcggga gcgtcgagcg tcgggttggg gttcctttgg ggcctgcaat
     9061 tccccgtcat caagaagatc tggacctctt cgttcgtgct ggtggcgggg ggttacagcg
     9121 cgcttctgct tgcggtcttt taccaggttg tcgaggtctg gcgctggcaa cgctgggccc
     9181 agccgtttgt ctggatcggc ctcaacccca tcacggccta cctcgccaat aacatcatcg
     9241 gcttccgccg cctagccacg cggttggcgg gaggcgatgt gaaaaacttc ttcaatgccg
     9301 tcatcgcccc cggctttggc gagctcatga tctcgatcgt gggattgggc ctcgcgtttc
     9361 tcctctgctg gtttctgcac cggcgaagaa tatacctccg cctctgattc ccgcagtcgt
     9421 cgaagtccga gagggctgcc ctgcgcgacc cagttgccta cttcccgggg cgccgcgccg
     9481 gcgtgaccca gcccttgtgc caatcgagac tccagagccg ttcgagcttc actgagtccg
     9541 catggccgtc cacataggaa atgttgatgg ctccgggcag gtcgttcttg ggattgaatt
     9601 gctggttctt gggccacggg ggcgcgccgc catgccgtgg cagggtcagg cggaccatcg
     9661 cgccggcgta atcgtcgccc gtgtagaggt tgcgcgccgg aggatcgttc ggctggggcc
     9721 aggtgtcgat ccagttgctg tcggcaatga ccggggtggt cgagggcgac gggcaatccg
     9781 tttcatttcg atacttcttc ggcttgtact cgacagtgct gaaaaacggg tcgtcgtccc
     9841 cgtaaaacca gccgttgaac gcatagctcc cctcgtaatc gatgcccttg gaccccgtcc
     9901 agagccaggt ctggttgagc gaaccgctgc tgggatggcg gcgcttggct ttcgtggggg
     9961 ccgacggagc cgccgggcaa atgcgtgcc
//
LOCUS       DAOQRO010000094         9962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6111_length_9962_cov_6.863228, whole genome shotgun sequence.
ACCESSION   DAOQRO010000094 DAOQRO010000000
VERSION     DAOQRO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6111_length_9962_cov_6.863228"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKK20_05155"
     CDS             complement(<1..1006)
                     /locus_tag="PKK20_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNU99305.1"
                     /translation="MKLRIWRYDLQLAHRWTIARGLEPGGPGGKTTSQVVFAELTDND
                     GLVGLGEAAPSARYGETTDAAVAFLERVDANRLSFADLASSLEYLRSLAPGQFAAKGA
                     LDVALLDGAARRGGRPLHTHLGLGFAENKHVTSFSIGIDTPEVITRKTRAAEAYPVLK
                     LKLGSPHDRDNLAALRAAAPHKPVRVDANEAWKTKEIALQNLEWLAQDGAIQFVEQPM
                     PASADLEDMVWLKARSPLPLMGDESYVSVKDVERCAAAFHSVNVKLVKVGGVSPGFAA
                     LHAARQAGLKTMLGCMIESSVLISAAAHLADLTDYLDLDGNLLVTNDPFCGVKADRGL
                     L"
     gene            1648..2712
                     /gene="argC"
                     /locus_tag="PKK20_05160"
     CDS             1648..2712
                     /gene="argC"
                     /locus_tag="PKK20_05160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNU99306.1"
                     /translation="MSARVRVGIVGASGYSGEELVRLLLGHPRVELTAVTSRQYAGRT
                     VAQVFPKFASHSGARTLRFSEPNVDLLATQAQVVFLALPHGVAAEFAVPLLQRGCQVI
                     DLSADFRLRSPDVYREFYAHDHPAPDLLAGAVYGLVEVYRDTLRGAALVASPGCYPTS
                     ILLPSIPLLRQQLVKTSGVIANSLSGVSGAGRKAEIDYLFVECNESMRPYAVPKHRHL
                     SEIEQELSAAAGAPVVIQFTPHLIPVSRGILTTLYFTPSQHVQGADQAAAFADTVAAC
                     YQAAYPDQPFVRLLQGNALPDTKNVVGTNVVEIAWRVDPRTGRLIVMSAVDNVVKGAS
                     GQAVQCFNVLSGFDETTGLL"
     gene            complement(2714..4213)
                     /locus_tag="PKK20_05165"
     CDS             complement(2714..4213)
                     /locus_tag="PKK20_05165"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HNU99307.1"
                     /translation="MNGEASSWTLPEDSEVWYQTNTGDYEGVYQRRRPALIPLEERTA
                     RGTNAVHLGPPITVELARGGCLLVTEANLFGYSGMTLRPSGTGRIQAAFEDDPGGFEI
                     EGEILSPWRVTVAVPDLQSLVNTDVIHNLCEPPDPRLFPDGAKTKWIEPGRALITWCV
                     FGNDGAQWHLQKWFVDQCAALRCEHLLVDAGWQSERWGWLADGGDLWARLQELCEYGA
                     RKGVGIVLWHAFPEGRDDGPGLTRPEARRELFGRCREAGVKGVKIDFFNSERKEIVEA
                     YEDLTRLAAEHRLTINFHGANKPTGEVRTWPNEITREGIREQEYVLWDKLPLEHYAAL
                     PFTRMVVGHSDFLPTYVRAKYLKNTTAALQMATAIIATSSFLCWPDHPDDYLASPFLG
                     LIQALPVVWDETIVLPGSRIGRLAAFARRAGDAWYLGVLNGTDQRVEWEAPADFLGAS
                     EFLATLYRDAAPHPTGVAVVTGHPVRRGDVLRAELAPGGGFVAWLRPKP"
     gene            complement(4210..4635)
                     /locus_tag="PKK20_05170"
     CDS             complement(4210..4635)
                     /locus_tag="PKK20_05170"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HNU99308.1"
                     /translation="MRSQIPISAVVVALWIISPALGAEFVSPGGGLSARIEPDPEGRL
                     VWSLRRGEAILIEPSRLGISVNGTDLGVGVTLGGVSIREIDERYAWRGVKAQATNRCR
                     VHALAVRHGVSGVEWQIEARLFDDGLPAVTGCPDTAYGR"
     gene            4732..4914
                     /locus_tag="PKK20_05175"
     CDS             4732..4914
                     /locus_tag="PKK20_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99309.1"
                     /translation="MQGSRPFANVPPVYPTLPNQLVLDGAWHDGASGLRSPLINPAIE
                     QPFAEVAVDDLADSRP"
     gene            4943..5083
                     /locus_tag="PKK20_05180"
                     /pseudo
     CDS             4943..5083
                     /locus_tag="PKK20_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5405..7963
                     /locus_tag="PKK20_05185"
     CDS             5405..7963
                     /locus_tag="PKK20_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99310.1"
                     /translation="MKPFRRVFGPGLIRLGLRAVTVLLVSAGLTRAQETAPAFIQELN
                     LVHFCHTDIGFTDHPAVARELHRRYLDVALDAVLARLDGPPEKRLCWTAETTLAVTDW
                     WEAAPPERRRQFLEAVREGVLDVAALPFNQTPFLDAAQWDTMMRWMPEDLWRELGPSV
                     AIQNDVNGFPRAGARRLLDRGVHRLFMGINDDSGGAPFPRHSAFWWRQPDGRRMFVWL
                     NAHYGSGFSLFESDEWRRGPVPYASDTRYRPPRAGDILRSDEASVRAAHAICRGKLRQ
                     IEDGGYTNAVLTTSITSLWRMDNDPPFPPLADFVATWNRLGLKPYLHLTTASAAAAQM
                     EEILGPTAPEYAGEWTDWWANGTASAPREVAASRVAKRALLAVRSPLWGAIGSEVRMS
                     VDSLYRDLCLFDEHTWGSSWSIGMPYILDTQAQFNEKASFAFRPMARAEWLLSQHVRT
                     RLANASEGLFLANPTDAPFTGWVSIPTPALRQDYQSVQDPESGARQRLEFDPGVAAWG
                     RPQRPENLTYENAAATFADALPRRFARFWVEALPAHGIRRLVLSTEPVTQEPTPTGPG
                     PEVERDGQGWPMTAQWPGLSKPLFLPVLGDFVAVQVDGFAPRWVTMDIMNERDNDDRR
                     DRMRRERLREVRAQPAAATDFSETPHTLRFTQRLDHPRLAWATRQLELWKGSPRARLT
                     FRLNRISSADPEIFYIATALPTGELMPRLTCGGESFTPFNDQLPGTCRDYFAVDGWAD
                     YATPEGRWLWVSRDAPLLTLGSPQVLARRRTPVADPHRLLSMVYNNFWHTNFAADESG
                     VMEFQFDLAWLPPTTTPDHVDRLAVSLPSNPIVIINPAGNEDRHLMKWLFAPGR"
     gene            complement(8049..8390)
                     /locus_tag="PKK20_05190"
     CDS             complement(8049..8390)
                     /locus_tag="PKK20_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNU99311.1"
                     /translation="MSKTLFERICTKEIPVKIVYEDDLVVAFHDIHPAAPTHILIVPR
                     RPVPSVGTATPEDQALLGHLLLKAAEVARLAGLTNGYRLVFNNGPDAGEAVPHLHCHI
                     LGGRSLGWPPG"
     gene            complement(8387..8941)
                     /locus_tag="PKK20_05195"
     CDS             complement(8387..8941)
                     /locus_tag="PKK20_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99312.1"
                     /translation="MTPQPTACLVFKGASDVIACGAAGVSIELVGEGLNGCESDLVRE
                     VASGVLHYFRHDLGRDTVTVGEFAVVLAKVLRGFGLNVESCLRVPDATPAAEADLTDL
                     IVEGGGTFELEFYQRLRKELARQLEPGPRVVRFTGLRVCVKKLAGARRWSGRCEELSD
                     QIVEYLRRCLHAERNAGHCPVIVV"
     gene            complement(8907..9392)
                     /gene="nrdR"
                     /locus_tag="PKK20_05200"
     CDS             complement(8907..9392)
                     /gene="nrdR"
                     /locus_tag="PKK20_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNU99313.1"
                     /translation="MRCPKCGGLNDKVIDSRASREGSTIRRRRECLHCEHRFTTYEAI
                     ETQTLMVIKRDGRREEFSRDKLLNGIRKACQKRPVSPQAIEEIVDRIVVDLHDGYEGE
                     VPCMAIGERIMDELRVLDGVAYVRFASVYRRFEEATDFVQAVQKLEVRHDTATYRLPG
                     F"
     gene            9729..>9962
                     /locus_tag="PKK20_05205"
     CDS             9729..>9962
                     /locus_tag="PKK20_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99314.1"
                     /translation="MNRVLYVGWLWVVLGLSPSTASAAGQSAANAIVAIVDDEVITWS
                     DVQQEVEPVIELKLGLLANRRAELEREIQKAQQE"
BASE COUNT         1759 a         3318 c         3077 g         1808 t
ORIGIN      
        1 agaggagacc gcggtcggcc ttcaccccgc agaatgggtc gttggtgacc agcaagttgc
       61 catccagatc gaggtagtcg gtcaaatcgg caaggtgcgc ggccgccgag atcagaacac
      121 tcgactcgat catgcagccg agcatggtct tcaggcctgc ctgccgggcc gcgtgcaagg
      181 ccgcgaaacc cggggacaca cctccgacct tgacgagttt cacgttcacg gagtgaaacg
      241 ccgcggcgca gcgctcgacg tccttgacgg agacatacga ctcgtccccc atgaggggca
      301 ggggcgaacg cgccttcagc cagaccatgt cctcgagatc ggcgctggcg ggcatgggtt
      361 gttcgacgaa ctggatagcc ccgtcctggg ccagccattc gagattctgg agggcaatct
      421 ccttggtttt ccaggcttcg ttggcatcga cgcggacagg cttgtgcggg gctgccgcgc
      481 gcaaggccgc caggttgtcg cgatcatgcg ggcttcccag tttgagtttc aacaccggat
      541 aggcttccgc cgcgcgggtt ttccgcgtga tgacctcggg ggtgtcgatg ccgatgctga
      601 acgaagtgac atgcttgttc tcggcaaagc cgaggccgag gtgggtgtgc aacggtctgc
      661 cgccccgccg cgccgcgcca tcgagcaaag caacgtccag cgcgcctttg gcggcgaact
      721 gacccggcgc gagacttcgc aggtattcga ggctcgatgc caggtcggcg aaggagaggc
      781 gattggcgtc cacccgttcg agaaaggcga cggcggcatc ggttgtctcg ccgtagcggg
      841 ctgaaggcgc cgcctcgccg aggccgacga gcccgtcgtt gtccgtcagt tcggcgaaaa
      901 ccacctggct ggtcgtcttg ccgcccgggc ctcccggctc gagcccgcgg gcgatcgtcc
      961 aacgatgtgc caactgcaag tcgtaacgcc agatgcgcaa cttcatggcc ggtacagaag
     1021 ccgagatgga gcgccgatgc aactccagaa gagcttgtga gggctcgggg cgtggggatt
     1081 cacgggctgg gctgcggcgg ttctcatttt ggtcacgagc cgtctagcgg tggttggtga
     1141 gctcacgggt tgggttttcg ggttggctct ggtggtcctc aaacggctgg tccaaggagg
     1201 gcaaattttc gccgctttga tgcgcccgcg cttgcattcc cgcggtccct tggtcaaggt
     1261 cgatgcctgg gcgccgcaaa cacaggccgc ccggtcgaac tttcaatgaa ccgtggttcc
     1321 gtagccgcgg acgtgagtcc gcgccatctc gctccgtcaa aagcagaaat cagcgccgac
     1381 tcacgtcggc ggctacaaat cggttcatgg tccccgtgcg cattcgaccg ctgaatgtca
     1441 aaacttccta tgaaccgtgg ttccgtagcc gcggacgtga gtccgcgcca tctcgctccg
     1501 tcaaaagcag aaatcagcgc cgactcacgt cggcggctac aaatcggttc atggtccccg
     1561 tgcgcattcg accgctgaat gtcaatactt cctatgaacg gatcggtgca tggtacggat
     1621 gaaacttcgc cctcctcggc cggcggcatg tccgcgcggg ttcgggtcgg gattgtcgga
     1681 gcatcaggct actcgggcga ggaactggtg cggttgttgc tggggcatcc gcgcgtcgaa
     1741 ttgacagccg tcacttcccg gcaatacgcc ggccggaccg tggcccaggt tttccccaag
     1801 ttcgccagcc actcgggtgc ccgcacgctg cggttcagcg agccgaacgt cgatctcctc
     1861 gccacccagg cccaggtcgt gttcctcgcc ctgccgcacg gcgtggccgc cgagttcgcc
     1921 gtgccgctcc tgcaacgcgg atgccaggtg atcgatctga gcgccgactt ccgactgcga
     1981 agcccggacg tctaccgcga gttctacgcg catgaccacc cggcgccgga cctcctggcc
     2041 ggcgctgtgt acgggctggt cgaggtctat cgcgacacac tgcgcggcgc cgccctggtc
     2101 gcgtctccgg gatgctatcc gaccagcatc ctgctgccgt ccatcccgct cctccgccag
     2161 cagttggtga aaacgtccgg ggtgatcgcc aattcgctca gcggggtgag cggtgcgggc
     2221 cgcaaggccg agatcgatta cctgttcgtc gagtgcaacg agagcatgcg gccctatgcg
     2281 gtccccaaac accgccacct ctccgaaatc gaacaggaac tctccgccgc cgcaggcgcc
     2341 ccggttgtca tccagttcac gcctcatctc attcctgtca gccgcggcat tctgacaaca
     2401 ctctacttca cgccttccca acacgtgcag ggcgcggacc aggccgccgc gtttgcggac
     2461 acggtagccg cctgttacca ggctgcctac ccggaccagc cgtttgtccg actgctccag
     2521 ggcaacgccc tgcccgacac gaaaaacgtg gtcggcacca acgtggtcga aatcgcctgg
     2581 cgcgtcgatc cccgcaccgg acgcctcatc gtcatgagcg cggtggacaa tgtggtcaag
     2641 ggcgccagcg gccaggctgt gcagtgcttc aacgtgctgt ccggattcga cgagacgacc
     2701 ggcctgctgt agatcacggc ttgggtctga gccaggccac aaacccgccg cccggagcca
     2761 actccgcgcg caggacatca ccgcgccgca cgggatgtcc ggtcacaacc gctaccccgg
     2821 tcggatgggg cgcggcgtcg cgatagagcg tggccaggaa ctcggacgca ccgagaaaat
     2881 cggctggggc ttcccactcg acacgctgat cggtcccatt cagcacgccc aaataccacg
     2941 cgtcgccggc gcgccgggca aaggccgcca gccgcccgat gcgcgagccg ggcagcacaa
     3001 tcgtctcatc ccaaaccacc gggagcgcct ggatcaaccc gagaaacggg ctcgccaggt
     3061 aatcgtccgg gtggtcgggc cagcagagaa acgacgaggt cgcaatgatg gccgtggcca
     3121 tctgaagcgc ggctgtcgtg ttcttcaggt acttggcccg aacatacgtc gggagaaaat
     3181 cggagtgccc gaccaccatc cgggtgaacg gcagcgcggc gtaatgttcc agcggcagct
     3241 tgtcccaaag cacatactcc tgttcgcgaa tcccctcccg cgtgatctcg ttcggccagg
     3301 tacgcacctc gcccgtgggt ttgttggccc catgaaagtt gatcgtcagc cgatgctcgg
     3361 cggcgagccg ggtgaggtct tcgtaggcct cgacgatctc tttgcgctcg gaattgaaga
     3421 agtcaatttt gacgcctttg acgcccgcct ctcggcagcg gccaaacagt tcccggcgcg
     3481 cctcgggtcg ggtgagcccc ggaccatcgt cacgcccttc cggaaaggcg tgccacaaga
     3541 caattccgac ccctttgcgc gccccgtact cgcacagttc ctgaaggcgc gcccagaggt
     3601 cgccaccgtc ggccagccat ccccaccgct cgctctgcca cccggcgtcc accaacagat
     3661 gctcgcagcg aagcgcagcg cattgatcca caaaccactt ctgcaaatgc cactgcgcac
     3721 cgtcattccc gaacacgcac cacgtgatca gggcgcgccc gggctcgatc catttcgtct
     3781 tcgctccgtc cggaaagagg cgcgggtccg gtggctcgca gagattgtgg atcacgtcgg
     3841 tgttgaccag gctttgcagg tccggcacgg ccactgtcac gcgccacggc gagagaatct
     3901 ccccctcgat ctcgaagccg cccggatcgt cctcgaaagc ggcctgaatc cggcccgtgc
     3961 cgctcggacg cagcgtcatg ccgctgtacc cgaacaggtt cgcctctgtt accagcaggc
     4021 agccgccccg cgccaattcg actgtgatag gcggccccag gtggacggca ttggttccgc
     4081 gcgcggtccg ctcttcgagc gggatcaggg ccggacgacg ccgctggtag acgccctcgt
     4141 aatcgcccgt gtttgtctga taccacacct ccgaatcctc cggcaaagtc caactcgacg
     4201 cctccccgtt caccgtccgt acgccgtgtc cgggcacccg gtaacggcag gcaacccatc
     4261 gtcgaacagc cgcgcttcga tctgccactc cacacccgag accccgtgcc ggacggccaa
     4321 tgcatggacg cgacaccggt tggtggcttg ggccttcacc ccgcgccagg cataccgctc
     4381 atcgatctcc cggatgctca ccccacccag cgtcaccccg acacccaaat ccgttccatt
     4441 gaccgatatg cccagccgcg agggctcgat cagaatcgct tccccgcggc gcaacgacca
     4501 gaccaggcgc ccttcggggt ccggctcgat ccgggcgctc aacccgccgc cggggctgac
     4561 gaactcggca cccagggccg gagagatgat ccataaggca accacaacgg ctgaaatagg
     4621 aatttgcgag cgcatgatga atcgccaatt ctgaactctt ggcggcctcg cggtcaagtc
     4681 gggggaaccg cagcccgagc aattcgtttc caggctgcca ggcattttcc cgtgcaaggc
     4741 tcacgaccct tcgccaacgt cccgcccgtg tatccgacat tgcccaatca gctcgtcctc
     4801 gacggcgcct ggcacgacgg cgcgtccggt ctgcgctctc cattgatcaa tcccgccatc
     4861 gaacagccgt tcgccgaagt ggccgtggac gatctcgcgg actctcgccc atgataccgg
     4921 tttgccaccc ggcgtcctcc aggtgctccc cggctccagc gcttccatcg gcaacgctct
     4981 ggtcacgctt ccgctcgtcc gcaaagtctt cttcatcggc tcgaccgaaa tcggtcggcg
     5041 gatcagggaa ctcgcctccc gtgatctgaa gcgcatgtcc atcgaactcg gcggtaaatc
     5101 gcccaatatc gtctttgccg gcgaagacgt agccgcgaaa ctcggagtga tccacgttgc
     5161 cgcgcaggat gtcggcggct ttttccagaa aactgtccgg ttccgcctgc gtcatccgcc
     5221 tcacggggcg ggtcttgatg ccattgctgg ccgtgaatcc catggaagcc atgctgcgcc
     5281 gagttctctg gtcgttttgc tttctttttg gaaaccggga cgatggccgg aaaggccggt
     5341 tcagaaccag aacgaagcgc taacgctgga cgtcgcggca aggggatggc tagggtgtgg
     5401 ccctatgaag ccatttcgaa gggtgtttgg ccccggactc attcggctcg gtctgcgggc
     5461 ggtgacggtt ctcctcgtga gcgccggcct gactcgggcg caggaaacgg cgccggcttt
     5521 catccaagaa ctgaatctcg tccatttctg ccacacggac atcggcttca cggaccatcc
     5581 ggccgtggcg cgcgagctgc accggcgcta tctggacgtt gcgctcgacg cggttctcgc
     5641 ccggctcgat ggtccccccg agaagcggct ctgttggacg gccgaaacca ccctggcggt
     5701 cacggactgg tgggaggccg cgccgcccga gcgtcgtcgc cagttcctcg aagcggtccg
     5761 tgaaggcgtt ctggatgtgg ccgccctgcc cttcaaccaa accccatttc tggacgcggc
     5821 gcaatgggac acgatgatgc ggtggatgcc ggaggatctg tggcgcgagc tgggaccatc
     5881 ggtggcgatt cagaacgacg tcaacgggtt tccccgtgcc ggcgcgcggc ggctcctgga
     5941 ccggggcgtg caccggcttt tcatgggaat caatgacgac agcggcggcg cgccgtttcc
     6001 ccggcactcg gccttctggt ggcgccagcc cgacggacgg cggatgttcg tgtggctcaa
     6061 tgcgcactac ggctccggat tctccctgtt cgagtcggac gaatggcggc gcggtccggt
     6121 cccgtacgcc tccgacaccc gctatcgccc gccgcgcgcg ggcgacatct tgcgcagcga
     6181 cgaggcctcg gtgcgggcgg cccacgcgat ttgccggggc aagctcaggc agatcgagga
     6241 cgggggctac accaacgcgg ttctgaccac ctcgatcacc agcctctggc gaatggacaa
     6301 tgatccgccc ttcccgccgc tggccgattt cgtggcgacc tggaatcggc tcgggctcaa
     6361 gccgtatctc catctcacga ctgcgtccgc cgccgcggcg caaatggaag agattctcgg
     6421 tccgaccgcg cccgagtacg cgggggaatg gaccgattgg tgggccaacg gcacggccag
     6481 cgcgccacgc gaggtcgccg ccagccgcgt tgccaaacgc gcgctgctcg cagtccgatc
     6541 gcctctgtgg ggagcgatcg ggtccgaagt ccgcatgagc gtcgactcgc tgtatcgcga
     6601 tctttgtctg ttcgacgagc acacctgggg ttcgagttgg agcatcggga tgccgtacat
     6661 cctcgacacc caggcgcagt tcaacgaaaa ggcctcgttc gcctttcgcc ccatggcgcg
     6721 ggccgaatgg ctcctcagcc aacatgtccg cacccggctt gcgaacgcca gcgaaggact
     6781 gtttcttgcg aacccgaccg acgcgccgtt caccggttgg gtcagcatcc cgacacccgc
     6841 cttgcgccag gactatcaat ccgtgcagga ccccgaatcc ggcgcccgtc agcgcctcga
     6901 gttcgatccc ggcgtcgcgg cgtggggtcg tccgcaacgc cctgaaaatc tcacatacga
     6961 gaatgccgcc gcgactttcg ccgacgcttt gcccagacgc tttgcccggt tctgggtcga
     7021 ggccctgccc gctcacggca ttcggcgtct cgtcttgagc accgagcccg tgacgcagga
     7081 gcccacgccg accggacccg gccccgaggt cgagcgggat ggccagggct ggccgatgac
     7141 cgcccaatgg cccggccttt ccaagccgct gttcctgcct gtgctcggcg atttcgtcgc
     7201 ggtccaggtc gacggttttg ccccgcggtg ggtgacgatg gacatcatga acgagaggga
     7261 caacgacgac cggcgcgacc ggatgcgacg cgagcggttg agagaggttc gggcgcagcc
     7321 ggcggctgca accgatttca gcgagactcc gcacaccctg cggttcacgc agcgactcga
     7381 tcatccgcgt ctggcctggg cgacccgcca gctcgaactc tggaagggca gcccgcgcgc
     7441 ccggctcacc ttccgactca accggatttc gtcggctgat cctgaaatct tctacattgc
     7501 gaccgccctg cccacaggcg agctcatgcc gcgtctgacc tgcggcggcg agtccttcac
     7561 gcccttcaac gaccagcttc ctggcacctg ccgggattac tttgcggtgg atggctgggc
     7621 cgattatgcc acgcccgagg ggcgttggct gtgggttagc cgggatgcgc ccctgttgac
     7681 gctcggttca ccgcaggtgt tggcgcggcg ccggacgccg gttgccgatc ctcaccgcct
     7741 gctgtcgatg gtgtacaaca acttctggca caccaatttc gccgccgacg aatcgggtgt
     7801 gatggagttc cagttcgatt tggcctggct gccgccaacg acgacccccg atcacgtcga
     7861 tcggctggcc gtctcgctgc catccaatcc catcgtgatc atcaatcccg ccgggaatga
     7921 agatcggcac ctgatgaagt ggctgttcgc gccggggcgc taagccgcat attccacgaa
     7981 ctgcactcca gaaggcgact ctcgacgaca ggccacgaca gagcctgccg tcaaggcgtt
     8041 cgagatggct aacctggcgg ccagcccagg gaccgtccac ccaggatgtg gcaatggagg
     8101 tgcgggactg cctcgccggc atcgggtccg ttgttgaaca caagcctgta gccgttggtg
     8161 aggccggcca ggcgagccac ctcggctgcc ttcaagagca gatgcccgag caaagcctgg
     8221 tcctcgggag tggccgtgcc cacggacggc acgggccggc gcgggacaat gagaatgtgt
     8281 gttggggcgg cgggatggat gtcgtgaaac gccacaacca aatcgtcctc gtaaacgatt
     8341 ttgaccggga tttcctttgt gcagatgcgc tcgaacaggg ttttgctcat acgacaatga
     8401 cggggcaatg gccggcattg cgctcggcat gcaggcaccg ccgcagatac tcgacaatct
     8461 gatcgctcaa ctcctcgcag cgtccactcc accgccgggc gccggccagc ttcttcacgc
     8521 aaacgcgaag acccgtaaaa cggaccacgc gggggcccgg ttccaactga cgcgcgagtt
     8581 ccttcctgag ccgctggtag aattccagct cgaatgtgcc cccgccctcg acgatcaagt
     8641 cggtcaaatc cgcttcagcc gcgggcgtcg cgtccggcac gcgcaggcaa ctctcgacgt
     8701 tgagaccgaa gccgcgcaag accttggcaa ggaccacggc gaactccccg actgttacgg
     8761 tgtctcgtcc gaggtcatga cggaaatagt gcaggacacc gctggcgact tcacggacca
     8821 ggtccgactc gcatccgttg aggccttcgc cgaccaactc gatgctgacg ccggccgcgc
     8881 cacaggcgat cacgtcagac gcccctttaa aaaccaggca ggcggtaggt tgcggtgtca
     8941 tgtcgcacct cgagtttctg gaccgcttgg acgaagtcgg tggcttcctc gaatcgccga
     9001 tagacgctgg cgaaccggac gtaggcgacg ccatcgagca cgcggagttc atccatgatg
     9061 cgttctccga tcgccatgca gggcacctcg ccctcgtacc cgtcgtgcag atcgaccacg
     9121 atgcgatcga caatttcctc gatggcctga gggctgacag gccgcttctg gcacgccttt
     9181 cgaatgccgt tgagcaactt gtccctcgaa aactcctcgc gccgcccatc ccgtttgatg
     9241 accatgagcg tctgcgtctc gatcgcttcg taggtggtga accgatgttc gcagtgaagg
     9301 cattcgcgac ggcgacgaat tgtagaccct tcccgggagg cccgtgaatc gatcacctta
     9361 tcgttcagac cgccgcattt cggacatctc atattctgcc acacgggatg gacactgccg
     9421 gatgtggctt gcggactggt atcacaccac gtgttggggt gtcaagcggc cgacacggat
     9481 ttcatgcagt ctcattttgt cctgcgcagc cgacgagaac cggctgaagc cgggataccc
     9541 aaccaggtcc gacgcttcgg cgctgataaa gtgacaggtt cgtaattatt gacacgcctt
     9601 ctgttttggc gtacatgtct caacaacagc aacccttcag gaatttaaat gaaataaccg
     9661 tgatgtggac tggtgcgggt tggaaaggac ttgctggacg gggctttggg tgggcaagat
     9721 cagggatcat gaaccgcgtc ctgtatgtcg ggtggctgtg ggttgttctg gggttgtccc
     9781 cgagcacggc gtcagcagcc ggccagtccg ccgcaaatgc gatcgttgcc atcgtggacg
     9841 acgaggtgat cacctggagc gacgtgcaac aagaggtcga gccggtgatc gagttgaagc
     9901 tcgggctgct tgccaaccgg cgagccgaac tcgaacgcga gatccagaag gcccagcagg
     9961 ag
//
LOCUS       DAOQRO010000095         9957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6116_length_9957_cov_5.243284, whole genome shotgun sequence.
ACCESSION   DAOQRO010000095 DAOQRO010000000
VERSION     DAOQRO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6116_length_9957_cov_5.243284"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PKK20_05210"
     CDS             complement(<1..1315)
                     /locus_tag="PKK20_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99315.1"
                     /translation="MKHTYDESKVKTLSSLEHIRLRTGMYIGRIGNGTHYDDGCYILL
                     KEVIDNAIDEYIMGYGREVRIDIEGQRVRVRDYGRGIPLGKVVDCVSKINTGAKYNDD
                     VFQFSVGLNGVGTKAVNALSKSFIVRSHRNGEFVEAAFRQGRLKAEKKGRNPKEPDGT
                     YIEFEPDPEIFKETEYRSEHIERRLRHYSYLNTGLKLVCGGTVFQSRHGLLDLVMEEL
                     GQDGSEPLYPPLHYASKTLEFCFTHCNSRYGETFYAFVNGQYTSDGGTHLSAFREGLL
                     KAINEFAGAKYEGDDVREAMIGAVSIRLKDPVFESQTKNKLGNTEIRTGLVNQVREEL
                     LHFFSRNKEIAQKILDKAKDTQQLRKELLEVKKLARERAKAISLRIPQLKDCKSHFDR
                     KRGKGQGTMVFITEGQSAAGSIVSCRDVNTQAIFVLRGKPLNVWDL"
     gene            complement(1682..3100)
                     /locus_tag="PKK20_05215"
     CDS             complement(1682..3100)
                     /locus_tag="PKK20_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU99316.1"
                     /translation="MSASLLSFALLLAAAGGQAGGAGDTNLPPWMMRPLSLSDCLNTA
                     LEQSAAVLKGRQDLEANHGLSVQTKAIVIPKLEASGAYSLVEDASVDRLTIAPNPAMP
                     GGFPVIDPGDRSWSTGIRLVQSIYEGGRMVSSLRTARLLREQALAQFHAVLADTAAEV
                     RIAYYDALLAEKQIVVNEESVALLEKEFEDSRRRFDAGTVPRFNVLRAEVELANARPG
                     LSRARNAYRIAKNVLLDRIGATVPSDVWEDIPLRLTGTLDTPQLRLDVPEAVQLALAR
                     RPELVVLRKSEDLRREGVVQARAGYLPRLEGFAGYGARKSMFSPDVADEVHGWEVGVQ
                     AVWSLFDGALTRGKVIEARAQLEKARVETEDVVRGIQLEVRSACSTLVEAWEVLESQG
                     KVLEQAHEALRLARVRAEAGSGTQLDVLGAQTALTQSGITQNRAKRDYAVARTRLERA
                     IGAYAPELEARVEPAPNDSALP"
     gene            complement(3544..5364)
                     /locus_tag="PKK20_05220"
     CDS             complement(3544..5364)
                     /locus_tag="PKK20_05220"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNU99317.1"
                     /translation="MHSHFCDSPFLYRRRPSREVTVGDPARNGVVIGGSRPVVKQSMI
                     TCDTMDTAECIRQTLELVAAGAQLVRITAPTVSAAANLEHIAAGLRAQGCWVPLVADI
                     HFKPDAALEAARWVEKIRVNPGNYADSKKFAVKEYTGAQYAAELARLDERFAPLVLLC
                     RQLGRAIRLGVNHGSLSDRIMNRFGDSPLGMVESALEFAGIARRHGFHNFLISLKASN
                     PKVVIECHRLLVARLAALGPDWNYPIHLGVTEAGDGEDGRIKSAIGIGSLLCDGIGDT
                     IRVSLTEDSPREIPVCTDLIAQVPALTLAASDAARLEPAPPVAPPALERLLAGIPMPA
                     DPYRYERRRSTQIPLAATVKCGGQQTVRVVVPRAAWNVLAPGLTPRSDAGPEAVYEDL
                     DLCQIDPTADFVLDRDDQLVTVQDGGGLPAIAAFRLLAARLAGMGRTNPILLKDRLDR
                     PAVPEPPRLALLRAAVVLGSLLADGIGDAILVRGDPRPVESLRLSYNILQAAGCRSFK
                     TDYVVCPSCGRTLFDLQAVTARVKARTDHLKGVKIAIMGCIVNGPGEMADADFGYVGG
                     APGKVNLYVGKNPVEFNIPEAEAVDRLVDLIRKSGKWLDP"
     gene            complement(5477..6895)
                     /gene="rseP"
                     /locus_tag="PKK20_05225"
     CDS             complement(5477..6895)
                     /gene="rseP"
                     /locus_tag="PKK20_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNU99318.1"
                     /translation="METAIKFALVALSVVLMFGASIFVHELGHFWVALRRRMRVEAFA
                     IGFGPKIWSRVRNGIEYSIRWIPAGGFVRLPQMFTSEALEGGDSSPGAPVPPAPAGSK
                     ILVSLAGPCMNLVFAFLIATVIYFVGLPVLVNPSVIGRLDSDSPEAKMGIREGDRIVE
                     FEGKPVASWEEINMAVALARTNIFSAVVERAGQRHTFELTAKFNPSIGLKWLNLDPRE
                     HPVVGAVESGMPAEKAGLKKGDRFVSFGDVPVVSQEHLIALVGKNEGKASAVVVERAG
                     ERVTLEVTPRYDPQTKRGRMGIVFASGVYTVMKPGPTPWVQVYSVWDRTISTLAALIH
                     SKETGVKASDLSGPVGILANMAIEANTDYRRALNFLVLLNVSLAILNLLPIPVLDGGH
                     IIMSIIEKLRRRPISIRFVEYTYTGFAILLISFMLYITFFDVRRLPLFKALLQRGAQV
                     VESVEPAAAGTPTPAPGPAPKP"
     gene            complement(6939..7358)
                     /gene="ruvX"
                     /locus_tag="PKK20_05230"
     CDS             complement(6939..7358)
                     /gene="ruvX"
                     /locus_tag="PKK20_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNU99319.1"
                     /translation="MRILALDHGSKRIGVAVSDELKVIAQPLEYIPASPFPEVLARLS
                     ELIRQYDVESILIGMPRNMDGSYGPAASTVREFAAAVGDAVAIPIRTWDERLTSVQAN
                     RFLTQANVRGRKRREKVDQTAAALLLQSYLDSCVDAV"
     gene            complement(7366..8658)
                     /gene="hemL"
                     /locus_tag="PKK20_05235"
     CDS             complement(7366..8658)
                     /gene="hemL"
                     /locus_tag="PKK20_05235"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNU99320.1"
                     /translation="MGGLARKKSDALFAEALERIPGGVNSPVRAFRAVGGKPFFVDRA
                     RGCRVWDVDGNEYIDYIGTWGPAIHGHAHPRIIEAIRSAAERGTSFGIPNPLEVKMAS
                     LICEAVPSVEKVRMCNSGTEACMAAIRVARGHTKRNKVIKFEGCYHGHVDSLLVNAGS
                     GALTFGHPDSAGVPAAFTQHTVVVRFNDIEAVKAAFLANSNDIAAVILEPVPGNAGLY
                     LPKPGFLTFLREITREEGAVLIFDEVMTGFRLARGGAQERFGVQPDLTCFGKIIGGGL
                     PVGAFGGRRDIMNCLAPLGPVYQAGTLSGNPLAMAAGIVALEMLGHETYNTLEKLGAQ
                     LEEGMLEAARAAGVPVQFLRCGSMFCCYFTDLPVHDLADAMKSDRDRFARYFHRMLDG
                     GISLAPSQFEAGFLCTAHTPADIERTVEAAARALRGLN"
     gene            complement(8682..>9957)
                     /locus_tag="PKK20_05240"
     CDS             complement(8682..>9957)
                     /locus_tag="PKK20_05240"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNU99321.1"
                     /translation="ENHPVEFNGLRLGLTICEDLWNDETFWPDRRYRRDPAVELVQAG
                     ADALFNISASPWHLGKNRVRHAMLARLAAAARRPLVYCNLVGGNDELVFDGRSLVFNR
                     HGAPIARGAAFEEDFVVANLESQTPLPPETLTDEEKLYRALVLGLGDYLRKCGFTQAV
                     LGLSGGIDSAVTACLAAAALGPENVHGVSLPSQFSSPGSLEDARALASNLGIRYDVIP
                     IQPAFESVLRSLNPVFAGRPADLAEENIQARLRGVALMALSNKFGGLLLTTGNKSEIA
                     VGYCTLYGDMCGGLAVLGDVPKTMVYRLARWINRERDLIPTASITKAPSAELRPNQTD
                     QDSLPPYDVLDAILEAYVVEGKTAGEIVGDGFDADTVKRMTELIDRSEHKRRQAAPGL
                     KVTSKAYGVGRRMPIAQRYRETSFGLGVGGSM"
BASE COUNT         1731 a         3239 c         3229 g         1758 t
ORIGIN      
        1 tgaggtccca gacgttgagc ggcttgcccc gaagcacgaa gatcgcctgc gtgttgacgt
       61 cgcggcagct tacgatcgaa ccggcggctg actggccttc ggtgatgaac accatggtgc
      121 cctgtccctt cccgcgcttg cggtcgaagt gcgacttgca gtccttcagt tgcgggatgc
      181 gcaggctgat ggccttggcc cgctcgcgcg ccagcttctt gacttcgagg agttccttgc
      241 gcagttgctg ggtgtccttg gccttgtcga ggattttctg ggcgatctcc ttgttgcggc
      301 tgaaaaagtg gaggagttcc tcgcgcacct gattgaccag gccggtccga atctcggtgt
      361 tgcccagctt gttcttggtt tgggactcga agaccgggtc cttcaggcga atcgagaccg
      421 cgccgatcat cgcttcgcgg acgtcgtcgc cctcgtattt ggcgccggcg aactcgttga
      481 tcgccttgag caggccctcg cgaaacgcgc tcaggtgcgt gccgccgtcg ctggtgtact
      541 ggccgttgac gaacgcgtag aacgtctctc catagcggct gttgcagtgg gtgaaacaga
      601 actcgagcgt cttggacgca taatgcagcg gcggatagag aggctcgctg ccgtcctggc
      661 ccagttcctc catcaccaaa tccagcaggc cgtggcgcga ctggaacacc gtgccgccgc
      721 acaccagctt caggccggtg ttcaggtagc tgtagtgccg cagccgccgt tcgatatgct
      781 ccgatctgta ctccgtctcc ttgaaaatct ccgggtccgg ctcgaactcg atgtatgtgc
      841 cgtcgggctc cttcggattc cttcccttct tttcggcctt gagccggccc tggcgaaacg
      901 cggcctcgac aaactcgccg ttccgatggc tccgcacgat gaacgacttg gagagcgcgt
      961 tgaccgcctt ggtgccgacg ccgttgagcc cgacgctgaa ttgaaagacg tcgtcgttgt
     1021 acttggcgcc cgtgttgatc ttcgacacgc agtcgaccac cttgcccagc ggaatgccgc
     1081 gtccgtaatc ccgcacccgc acacgctgcc cttctatgtc gatccgcacc tcgcgcccgt
     1141 agcccatgat gtattcgtcg atcgcgttgt cgatgacctc cttgagcagg atgtagcagc
     1201 cgtcgtcgta atgggtgccg ttgccgatcc gcccgatata catgccggtc cgcagccgga
     1261 tatgttcgag cgacgacaac gtcttgacct tgctctcgtc gtaggtgtgt ttcacgcctt
     1321 cagccttcct gatctgttgt aatcgtagtc gtaatccctg tcccgagctg cctttgtgat
     1381 atggatcacc gacgccttag ggtctgcgca aaattacttc cgattctggc gggagcgcaa
     1441 ccgggccaga tgcgaggccc gagggaggga gtgtatcctc tgagatactc gaccgaccga
     1501 ggaacgacgc agatgcccgg tgccgccccg ccccggaggg ctggggggct ttggctcgct
     1561 ggtcccgcgg acgcgggatt gctcctcggt cacagaccgc tgcgggtatg ctccctcgtc
     1621 gcgcctcgcc agcgagccaa aaccgctcca gcaaaatcgg aattgacttt tgcacagacc
     1681 ctcaaggcaa cgccgaatcg ttgggcgccg gttcgaccct ggcttcgagt tcgggtgcgt
     1741 aagccccgat cgcccgttcg agccgggtac gcgccacggc gtaatctcgc ttggcccggt
     1801 tctgcgtgat gccgctctgg gtcagagccg tctgagcgcc gagcacgtcc agttgggtgc
     1861 cgctgcccgc ctcggcgcgc acgcgcgcca ggcgcagggc ctcgtgcgcc tgttcgagga
     1921 ccttgccctg cgactcgagg acttcccagg cctcgaccag cgtcgaacag gcgctgcgga
     1981 cttcgagttg gatgccgcgg acgacgtcct cggtctcgac gcgcgccttt tcgagttggg
     2041 cgcgggcctc gatgactttg ccccgggtca acgccccgtc gaacagactc cagaccgcct
     2101 gcacgccgac ctcccagccg tgcacttcat cggccacgtc ggggctgaac atcgacttgc
     2161 gggccccgta tccggcgaag ccctcgaggc gcggcaggta tccggcgcgc gcctgcacga
     2221 cgccttccct gcgcaagtcc tcggacttgc gcagcacaac cagctcgggc cgccgggcca
     2281 gcgccaactg caccgcctcg ggcacgtcga gccggagttg cggcgtgtcc agcgtgcctg
     2341 tcagccgcag gggaatgtcc tcccaaacat ccgacggcac tgtggcgccg atgcgatcca
     2401 acagcacgtt tttcgcgatg cgatacgcgt ttcgggcgcg cgaaagcccc ggacgcgcat
     2461 tagcgagttc gacctcggcg cgcagcacgt tgaatcgggg caccgtgccg gcatcgaacc
     2521 gccgccggct gtcctcgaac tccttttcga gcagggccac ggactcctca ttgacgacaa
     2581 tctgcttctc cgcgagcagg gcatcataat acgcgatccg gacctccgcc gccgtatccg
     2641 ccaggacggc gtggaattgg gccagcgcct gctcgcgcag caaccgtgcg gtgcgcagcg
     2701 aagacaccat ccgcccgccc tcatagatcg actgaaccag acgaatgccc gttgaccagc
     2761 tccgatcgcc cgggtcgata acgggaaaac cgccaggcat tgcggggttg ggagcgatcg
     2821 tcaaccggtc gacagacgca tcttcaacca gggaataggc gccactggcc tcgagcttcg
     2881 ggatgacgat ggccttggtc tgcacgctca agccgtgatt ggcctcgagg tcctgcctgc
     2941 ccttgagcac cgcggcgctc tgttcgaggg ccgtgttcaa gcaatcggag agcgagagcg
     3001 ggcgcatcat ccagggcggg agattggtat cgccggcgcc gcccgcctga ccgcccgccg
     3061 ccgccagcag gagggcgaac gacaacagag aggctgacac cgcgccgcgc cggccggtta
     3121 ccgagaaatc ggcgagacgc actccaccgt caccttcacc cgtcccgcag agcgggacac
     3181 cctttcccac ctgcctgcgc ccaggcttcg gcaggcaggt cggatgggag agggccgggg
     3241 taaggggtct gtgtagggta tccagccgac tcggcagtat ttcaaacgct tcgtgaggaa
     3301 ccgtgaccgg gctggggtcc tcgaccggag agaagtgttg cagacaaggc aagtgtctgt
     3361 gctgcgtcaa agggattaag gattccaagg aacagggcgc gggacggagg ggcggcgaaa
     3421 cacgagtatt gctcatggaa aggaggactg ggtttggtct gaaacgcgtc tcgccagccc
     3481 gcatggcagc cctcttgcga agtctgagtg ccctgtgcac cataggggtg tgaaacatgc
     3541 gggctaggga tcaagccact tgccggattt gcggatgaga tcgaccagac ggtcgacggc
     3601 ttcggcttcg gggatgttga actccaccgg attcttgccg acgtagaggt tgaccttgcc
     3661 cggggcgccc cccacatacc cgaagtcggc atcggccatc tcgcccggcc cgttcacgat
     3721 gcagcccatg atggcgatct tcacaccctt caggtggtcg gtgcgcgcct tgacgcgcgc
     3781 ggtgaccgcc tgaagatcga acagcgtgcg cccgcacgac gggcagacga cgtagtcggt
     3841 cttgaacgag cggcatccgg ccgcctgcag gatattgtag gacaggcgca gcgactcgac
     3901 agggcgcggg tcgccccgga ccagaatggc atcgccaatc ccatcggcca gcagagaccc
     3961 aaggaccacc gcggcgcgca acagcgccag gcgcggcggt tccggaacag cggggcgatc
     4021 gaggcgatcc ttgagcagga tcgggttggt ccggcccatg cctgcaagcc gtgcggccag
     4081 gaggcgaaac gcggcgatgg cgggcagacc gcctccgtct tggaccgtca ccagttgatc
     4141 atcgcggtcc agcacgaagt cggctgtcgg atcgatctga cagaggtcga ggtcctcgta
     4201 cacggcctcc ggaccggcgt cgctccgcgg tgtgagcccg ggtgctagga cgttccacgc
     4261 ggcgcgcgga acgacgacgc gcacggtctg ttggccgccg catttcaccg tggccgcgag
     4321 cgggatttgc gtcgatctgc gtcgttcgta gcggtagggg tcggcgggca tagggatgcc
     4381 ggcaagcagg cgctcgaggg ctggcggcgc cacgggcggc gcgggttcca ggcgcgcggc
     4441 gtccgatgcg gccagcgtga gggccgggac ctgtgcgatg aggtccgtgc agaccgggat
     4501 ttcgcgcggc gagtcctcgg tgagcgagac acggatggtg tcgccaatgc cgtcgcagag
     4561 cagtgagccg atgccgatgg cgctcttgat gcgaccgtct tcgccatcgc ccgcttccgt
     4621 gaccccgagg tgaatggggt aattccagtc cggacccagt gcggccaggc gcgcgacgag
     4681 cagccgatgg cattcgatga ccaccttggg attggacgcc ttcagcgaga tcaggaagtt
     4741 gtggaagccg tggcgacggg cgatgccggc gaactcgagg gcgctttcga ccatcccgag
     4801 cggactgtcg ccgaaccggt tcatgatccg gtcgctgagc gagccgtggt tgacgcccag
     4861 gcggatggcg cgcccgagct gccggcagag gaggaccagc ggagcgaatc gctcatcgag
     4921 gcgggcgagt tcggcggcgt actgcgctcc ggtgtattcc ttgacggcga actttttcga
     4981 gtcggcgtag ttgcccggat tgacacggat cttctcgacc cagcgcgcgg cttcgagggc
     5041 ggcgtccggc ttaaaatgga tgtcagccac caggggcacc cagcagcctt gggcgcgcaa
     5101 tccggcagcg atatgttcga ggttggctgc cgcggacacc gtcggcgccg tgatgcgcac
     5161 cagttgagcg cccgcagcca ccagttcgag tgtttgccgg atacattccg ccgtgtccat
     5221 cgtgtcgcac gtgatcatcg actgcttgac cacgggacgg ctcccgccga tgaccacgcc
     5281 gtttcgagcc gggtcgccga cggtcacctc gcggctcggc cttcggcggt aaaggaaagg
     5341 ggaatcacaa aagtgcgagt gcattcgaaa gtgtctcccg ccgcagtcgc atacggtgcc
     5401 ttcgcgttcc cgtccgcatg ccgtcgctgc cgggcgctcg ggaacggtcc ctctgaccag
     5461 ggcggctcat gtcgcctcat ggtttcggag ccgggccggg cgccggcgtc ggcgtcccgg
     5521 ccgccgcggg ttcgaccgac tcgaccacct gggcgcctcg ctggagcagg gccttgaaga
     5581 gaggcaatcg gcggacatcg aagaaggtga tgtagagcat gaacgagatc agcaggatcg
     5641 cgaacccggt gtaggtgtac tcgacgaacc ggatgctgat cgggcggcgc cgcagcttct
     5701 cgatgatgga catgataata tggccgccat cgagcaccgg aatcggcagc aggttgagaa
     5761 tcgccaggct gacgttgagc aggaccagga agttcaaagc gcggcgatag tcggtgttcg
     5821 cctcgatcgc catgttggcc aggatgccga ctggtccgct cagatcgctg gctttcacgc
     5881 ctgtctcctt cgagtggatc agcgccgcga gagtcgagat cgtgcgatcc cagaccgagt
     5941 agacctggac ccacggggtc gggcccggtt tcatgaccgt atatacgccg ctggcgaaca
     6001 cgatgcccat ccgcccacgc ttggtttgcg ggtcatagcg gggcgtgact tcgagggtca
     6061 cccgctcccc ggcgcgctcg accaccaccg cgctggcttt gccttcgttc ttgccgacta
     6121 acgcaatcag gtgctcctgg ctgacaaccg ggacatcccc aaaggagaca aaccgatcgc
     6181 ctttctttag gcccgccttc tcggccggca tgcccgactc gacggcgcca accaccggat
     6241 gctcgcgggg atcgagattg agccatttga ggccgatgct cgggttgaac ttggcggtca
     6301 gctcgaacgt atgccgttgt ccggcgcgct cgacaaccgc gctgaagatg ttggtgcggg
     6361 ccagggcgac cgccatattg atctcttccc aggatgcaac aggcttgccc tcgaactcga
     6421 caatccggtc gccctcccgg attcccatct tcgcctcggg cgaatcggaa tcgagccgtc
     6481 cgatgaccga ggggttcacc aggacgggga ggccgacgaa gtagatgacc gtggcgatca
     6541 ggaacgcaaa caccaggttc atgcaggggc cggcgagcga gaccagaatc ttcgagcccg
     6601 cgggggcggg cggcaccggg gcgccgggag aggaatcgcc cccttccagg gcttccgatg
     6661 taaacatctg ggggagccga acgaacccgc cggccgggat ccaccgaatc gagtactcga
     6721 tgccgttcct gacgcggctc cagatcttgg gtccgaaccc gattgcgaac gcctcgaccc
     6781 gcatgcgacg ccggagcgcc acccagaagt gcccgagttc gtgcacgaaa atcgacgcgc
     6841 cgaacatgag tacgacggac agggccacca aggcgaactt gatcgctgtt tccatgtgtg
     6901 aatcgctcga ccgaccaaca agcgtcgaat cctacgtttc aaacggcgtc gacgcaactg
     6961 tcgagatagc tttgcaacag gagcgccgcg gcggtctgat cgactttctc cctccgtttc
     7021 cgcccgcgga cgttggcctg ggtgaggaag cgattggctt ggaccgaggt caggcgctca
     7081 tcccaggtgc ggatggggat ggcgacggcg tcgcccaccg cagcggcgaa ctcgcgcaca
     7141 gtcgaggcgg ccgggccgta actcccgtcc atgttccggg gcatgccgat gagaatcgac
     7201 tcgacgtcgt attgtcggat caactcgctg agccgtgcga gaacctcggg gaacgggctg
     7261 gctgggatat actcgagagg ctgggcaatc accttgagtt cgtcgctgac ggccacgccg
     7321 attcgtttgc tgccgtgatc aagggccagg atgcgcataa gagggtcagt tcaagccccg
     7381 caacgcgcgg gcggcggcct caacggtccg ctcgatgtcc gccggcgtgt gcgcggtgca
     7441 aaggaaaccc gcctcgaatt gggaaggggc aaggcttatc cccccatcga gcattcggtg
     7501 aaagtagcgg gcgaagcggt cgcggtcgct cttcatggca tcggccagat catgcaccgg
     7561 gaggtcggtg aaataacagc agaacatcga gccgcaccgg aggaactgga ccgggacgcc
     7621 cgcggcgcgg gccgcttcca gcattccctc ttcgagttga gcgcccagtt tttcgagtgt
     7681 gttgtaggtt tcgtgaccga gcatttcgag cgcaactatg cccgccgcca tggccagcgg
     7741 attcccgctg agcgtgccgg cctggtaaac cgggcccagc ggcgccaggc agttcatgat
     7801 gtcccgccgt ccgccgaatg cgcccaccgg gagtccgcct ccgatgatct tgccgaagca
     7861 agtgagatcc ggctggacac cgaaccgctc ctgagcgccg ccgcgggcca gccggaagcc
     7921 ggtcatgacc tcatcgaaga tcaggaccgc gccttcctcc cgtgtgatct cgcgcaggaa
     7981 ggtgaggaaa ccgggcttgg gcaggtagag gccggcgttg ccgggcaccg gttcgaggat
     8041 cacggcggca atgtcgttcg agttggccag gaacgccgcc ttcaccgcct cgatgtcatt
     8101 gaaacggacg accaccgtgt gctgggtgaa tgcggccggg acaccggcgc tgtccggatg
     8161 cccgaacgtc agggcccccg agccggcgtt gaccagcagg gaatcgacgt gcccgtggta
     8221 acacccttcg aacttgatga ccttgttgcg cttcgtatgc ccgcgcgcca cccggatggc
     8281 cgccatgcag gcctcggtgc ccgagttgca catccgcacc ttctcaacgc tcggaaccgc
     8341 ctcgcaaatc agcgacgcca tcttcacctc gagcgggttc ggtatgccga agctggttcc
     8401 ccgctccgcc gcgctccgga tcgcctcgat gatccgcggg tgcgcgtggc cgtgaatcgc
     8461 aggaccccag gtgccgatgt aatcgatgta ctcgttgccg tccacatccc acacccggca
     8521 tccgcgagcc cgatcgacga aaaacggctt gccgcccacg gcgcgaaacg cccggacagg
     8581 cgagttgacg ccgccgggga tgcgttcgag cgcctcggcg aacagggcat ccgacttctt
     8641 gcgcgcaaga ccacccatac atcaatcctc cattttcttc ctcacatcga gcctcccacg
     8701 cccaaaccga aagacgtttc ccgataccgc tgcgcgatgg gcattcgccg ccccacgccg
     8761 taggccttgc tcgtgacctt caaccccggc gcggcctggc gccgcttgtg ttcactgcgg
     8821 tcaatcaact cggtcatccg cttcaccgtg tccgcgtcga agccgtcccc gacgatttcg
     8881 ccggccgtct tgccctcgac cacgtacgct tccaggatgg catccaagac gtcgtagggc
     8941 ggaagcgagt cctgatcggt ctgattgggc cgcagctcgg ccgagggggc cttggtgatg
     9001 gacgccgttg ggatcaggtc ccgttcccgg ttgatccaac gggccaggcg gtagaccatc
     9061 gtcttgggaa cgtccccgag cacagccagc ccgccgcaca tgtcgccata cagagtgcag
     9121 tagcccaccg cgatctcgct cttgttgccg gtggtgagca ggaggccgcc gaacttgttc
     9181 gacagcgcca tcagcgcgac gccgcgcagg cgcgcctgga tattctcctc ggcgaggtcg
     9241 gccggacgcc ccgcaaaaac cggattcaga ctgcgcaaga cggattcgaa ggccggctgg
     9301 atcgggataa cgtcgtagcg aatcccaaga ttcgaggcca gggcgcgcgc gtcctcgagg
     9361 ctgccgggcg acgagaactg cgagggcagc gaaaccccgt gcacgttctc cggcccgagc
     9421 gccgccgccg ccagacacgc cgtcacggcc gaatcgatcc cgccgctcag tcccaggacc
     9481 gcctgggtga acccgcattt gcgcaggtag tcgcccaggc cgaggacaag cgcgcgatag
     9541 agcttctctt cgtccgtcaa agtctctggc ggcaggggtg tctggctttc gagatttgcc
     9601 accacaaaat cctcctcgaa ggccgcaccg cgggcgatcg gcgccccgtg gcgattaaaa
     9661 accaggctcc tgccgtcgaa taccagttcg tcattgccgc ccacgaggtt gcagtagacc
     9721 agcgggcgcc tggccgcggc ggcgagccgg gcgagcatcg cgtgccgcac ccggttcttg
     9781 cccaggtgcc acggcgaggc cgaaatgttg aagagcgcgt cggcgcccgc ctgcaccagt
     9841 tccaccgccg ggtcgcgccg gtaccggcga tcgggccaga acgtctcgtc attccagaga
     9901 tcctcgcaaa tcgtcaggcc cagccggagt ccgttgaact cgaccgggtg gttctcg
//
LOCUS       DAOQRO010000096         9868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6193_length_9868_cov_5.009885, whole genome shotgun sequence.
ACCESSION   DAOQRO010000096 DAOQRO010000000
VERSION     DAOQRO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6193_length_9868_cov_5.009885"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PKK20_05245"
     CDS             complement(<1..879)
                     /locus_tag="PKK20_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HNU99322.1"
                     /translation="MLAVCTLLSGATAATQAIPPDRAPDLSLRNEVQHAIDRGLTWLL
                     ARQDAAGWWSTADHPALTALVVSGFLGDPSGRYRTNPPPALLKAIDYLASSAHPDGSL
                     HRGSLINYNTAITSTALALANRPEHHDLLRRAHAFLARSQNDFGEPGKLDTPLDGGVG
                     YGDKNQHADLNNTLVALEALRATDHLVQDRLPAGTRPLNWDAAIQFIQNCQNLPSHNP
                     QPWVSDAPEDRGGFVYYPGYSMAGGVTNAATGRVALRSYGSISYAGLLSYIYARLERN
                     DPRVVGAVDWLRAHYTL"
     gene            complement(1048..1563)
                     /locus_tag="PKK20_05250"
     CDS             complement(1048..1563)
                     /locus_tag="PKK20_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99323.1"
                     /translation="MRTTVVIARAIAVGLLTCATWTGLLAQTTLEDMVDQGNASWMLG
                     KWQGSTDDATTVTLEFSWDLNKHVVAMHGKVGSDMEFKGYSALEPDTSEVKYTGYDNR
                     GALSRGTWNLEAGELTLRLESKTADKNTKMAAVFTGSPAQGLEVRLHGVSDYGSISYP
                     ARATLKLKKQN"
     gene            2044..3789
                     /locus_tag="PKK20_05255"
     CDS             2044..3789
                     /locus_tag="PKK20_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HNU99324.1"
                     /translation="MAIAASPDGATLFVAAGTAGRVLIVDAASGQVWSATALGGHANA
                     LALAPDGTRLIVATGEASGQIEVCDARSGRVERLVRAGHTPSALAVSPDGLRLYVANR
                     FNHDVSVIDWKGGRELGRIPVVREPVALVLTPDGRRLFVGNHLPAGRADAAEIAAAIT
                     VIDTERLEPAAQIQLPNGSTAVRGLAMSGDGEIVYATHGLGRYQMPTTQLDRGWMNTS
                     ALSVIDAAKAERINTVLVDDVDLGAANPWGVACSSDGRWIVVSHAGTHEVSIIDQPGL
                     IEKLRRIGAGERVDGASLSSEDVPNDLSFLVGLRRRVNLSGEGPRGVVLVGERLFVAE
                     YYSDSLGLLDLATAGPLRAKSFPLGPAVAPTQARRGEMLFNDARLCFQHWQSCASCHP
                     DARVDGLNWDLLNDGLGNPKNTKNMLLSHETPPAMIMGVRETAEDAVRAGIRHIQFLV
                     RPEEEAVAIDVYLKNLRPGLSPHRQGKDLSAAAQRGQQIFQRAGCAECHPPPLYTDLR
                     RHDVGTGTGREQGKSMDTPTLIECWRTAPYLHDGRAATMRDVLGEANPDDRHGRTSGL
                     TPIELSDLAEYVLSL"
     gene            complement(3877..5115)
                     /locus_tag="PKK20_05260"
     CDS             complement(3877..5115)
                     /locus_tag="PKK20_05260"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNU99325.1"
                     /translation="MNLDSLYSELTIPTKAKLALIVLDGLGDLAIVGQGEITPLEAAR
                     TPNLDAFVAQGCAQGRMIPVAQGITPGSGPGHLALFGYDPIEYQVGRGVIEALGLGVQ
                     LKAGDVAARANFCSLDPKGIIIDRRAGRISTDVCEELCGILSRGIKRAGPKGSEVEVI
                     IQPGKEHRFVVVFRGEGLAGPLSSTDPNREGQPIAIAKPENEKSVAQVKTAKIVADFY
                     KRALPLLAKHPRANGFLMRGIAHQPRIPLFQDRYQLKAACLAVYPMYKGLAQLVGMTK
                     IEGPKTIAEQFERYLAEYDNYDFFFIHYKYTDKAGEDGNFEAKKKAIEDFDAALPILL
                     RKKPDVIAITGDHSTPCAVKGHSWHPQPVLVHSALSGSDKLERFTETGSNMGSLGVFK
                     AKYLIRLMQANARMFDKFGA"
     gene            complement(5223..6275)
                     /gene="trpD"
                     /locus_tag="PKK20_05265"
     CDS             complement(5223..6275)
                     /gene="trpD"
                     /locus_tag="PKK20_05265"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNU99326.1"
                     /translation="MLERLTAHVRSGLAMPGDEAAAAAACLLDESLSIETKADFLSAL
                     ARKGETADEIAAFARFLRGKAIAPPLDTTLRNGGILDVVGTGGDRAGTFNISTAAALV
                     AASAGVKVAKHGNRAVTCQTGSADVIEALGIPIDLPPERAVSALRDHGFAFFFAPLYH
                     PAFRHIAPVRRLCADRGQRTLFNYLGPLLNPVCPTAQVMGVPRPDLCEPLAMVLQSLG
                     VQRGIVVCGRTGLATGAMYFDELSVLGDTTIAEFYQERGVHCSTLRRADFPMQPAILT
                     DLQGGDRATNAETLRRVLRGDERGPKRDAVLLNAGAALFVADRVRTVADGWEAAADLI
                     DSGRAWAKLEEIRISR"
     gene            complement(6403..7776)
                     /locus_tag="PKK20_05270"
     CDS             complement(6403..7776)
                     /locus_tag="PKK20_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99327.1"
                     /translation="MQHDLAQQSAGGESRRSFIRRTATATAAVAAVNMFKTPVYGQTQ
                     APSTGRVIGANDRIAVGFVGVGPQGMLHVNKMKQNADKNNVALAAVCDLWEKRREAAK
                     AVMGDCKTYDDYRKMLENKDIDAVLCATVDHWHAQISIDSMKAGKHIYVEKPMSRYLP
                     EAFEVWDTVKATGKVFQVGSQGCSDAKWHKCAELIKAGSLGPLVLGQGSYMRNSPAGE
                     WNYTIDPDLKAGSVQWDQWLGPNIKTRADFSADHFFRWRKYYPYCAGLLGDLFPHRLH
                     PLVLATGNPEFPNRVVAVGTKAIHTDKNTPGTQERDCPECLTLVAEFPSGYSLMVCSS
                     SVNEYGLPDVIRGHYATLLIGGANVDLKPEAKFSEEIDPESFKNLLPVEDIGEHESNW
                     ISCIRSGKQPNANIDLAVRVQAIVSLGEMSDRLGIACHYDEKTRTVMDGTGKKVDPVT
                     YGTLKPS"
     gene            complement(8230..8514)
                     /locus_tag="PKK20_05275"
     CDS             complement(8230..8514)
                     /locus_tag="PKK20_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99328.1"
                     /translation="MARWVQKLADALRTYGSDLLTGNSTSFAELANCRRKEARTYALR
                     ERLRAARPNPDAAWRRRDYATVVKALKPLRAALTAAEVGKLEYAESRCRK"
     gene            complement(9096..9539)
                     /locus_tag="PKK20_05280"
     CDS             complement(9096..9539)
                     /locus_tag="PKK20_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99329.1"
                     /translation="MLYAWKESKLIYKDRIVRVHIDSRSLKEGFTMSLCPDDGWCVDG
                     KSTSLSVDDIVRVTEKVVRYMEGYRMDLYVVVREDCEQSVAAFRRLQEILAKDPDWAR
                     PQAGKPQVELRTQTSGFGTEDAEALMAGQDSTTCVRGIRDEQTEE"
BASE COUNT         1721 a         2970 c         3224 g         1953 t
ORIGIN      
        1 gagcgtgtag tgggcgcgca gccaatcgac ggcccccacc acgcgcggat cgttccgctc
       61 gagacgggcg tatatgtagc tgagcagtcc cgcgtagctg atgctaccgt aggaacgaag
      121 cgcgacgcgt ccggtggcgg cgttggtcac tccgcccgcc atgctgtatc cgggataata
      181 gacgaacccg cctcgatcct cgggcgcgtc ggagacccag ggctgagggt tgtggcttgg
      241 gaggttctgg cagttctgga tgaactggat ggcggcgtcc cagttcaggg ggcgggtccc
      301 ggcgggcaac cgatcctgga ccaggtgatc cgtcgcacgc aatgcctcga gggcgacaag
      361 cgtgttgttc aggtcggcat gctggttttt gtcgccgtac ccgacgcctc catccagggg
      421 cgtatcgagt ttgcccggct cgccgaagtc attctgcgaa cgcgccagga atgcatgggc
      481 gcgtcggagc aggtcgtgat gctcgggtcg attggcgagg gccaacgcgg tcgatgtaat
      541 cgccgtattg taattgatca ggctgccgcg gtggaggctg ccatcgggat gggcgctcga
      601 cgccaggtaa tcgatcgctt tcagcagggc ggggggagga ttggtgcggt agcgaccgct
      661 gggatcgccg aggaagccgc tgacaaccaa tgcggtcagg gccggatgat ccgcggtcga
      721 ccaccagccg gcggcatcct gccgcgccag gagccaggtc aacccgcgat cgatcgcatg
      781 ctgcacttcg ttgcgcagcg agagatcggg agcgcggtcg ggaggaatcg cttgagtcgc
      841 cgcagtcgct cccgagagga gcgtgcaaac ggccaacaca atccatgccg cacgattggc
      901 ggtccgacac cttgtcgcaa acgagcgcat cgccgcccag tctactgcaa gaggcggggc
      961 ggagccaagc cgggaccgga gctaaatgaa agggatgaca cacgcatatt cagatgcgat
     1021 cccgttgcct taccgggcga gttcggctca attctgcttc ttgagcttga gcgtggcgcg
     1081 agcggggtag gagatgctgc cgtagtcgct gacgccgtgc aatcggactt cgaggccctg
     1141 ggctgggctg ccggtgaaca cggcggccat cttggtgttt ttatcggcgg tcttgctttc
     1201 gaggcgcagg gtcaattcgc cggcttccag attccacgtg ccgcggctga gagcgccacg
     1261 attgtcatag ccggtgtatt tgacctcgga tgtgtcgggt tcgagagcgc tgtagccctt
     1321 gaattccatg tccgaaccca ctttgccgtg catcgcgacg acatgcttgt tgaggtccca
     1381 cgagaattcg agagtgaccg tggttgcgtc atcggtcgat ccctgccatt tgccgagcat
     1441 ccaggaggcg tttccctggt cgaccatgtc ttcgagggtg gtttgggcca ggaggccggt
     1501 ccatgtggca catgtcaaga gtcccacggc gatcgctcga gctattacaa cagtcgtcct
     1561 cacagcagtg atcctttctg tgttgcgttt tagggtcccg ccactggacc gggcggcggc
     1621 atgccgacgg caggcggaga ccggcgcact gtagccggtc tggacgattg cgcaaggtct
     1681 ggtgtcggtt tgtgtcggat cggcagcgcc aattcatgaa gtcgaaaagg tcggagtggg
     1741 gacggctcgt cccccgccat gtcaagtgaa gaaggcgtgt gtcggcgacg gggctgtcgc
     1801 cgctccactc catgaatcgg ccctgtcgga tcggacgggt gccatgtttc gcttgaggaa
     1861 gagccgattt ttccactacc gtccgacccc atgcacgtcc gcttgaccag tccgcgcgtt
     1921 gtgggggctt ttgtacgcac tgcctgtcgc cccggttggt ggcgggtcga gatttgcttg
     1981 tcgctttgcc tttgggctgg ggggtgggcg cgggcggctg acacggaaca atatctttcg
     2041 ccggtggcaa tcgccgcctc tcccgatggc gcgacccttt ttgtggctgc cgggacggct
     2101 gggcgggtgc tgattgtcga cgccgcgagc ggccaggtct ggagcgcgac cgcactcggg
     2161 ggccatgcga acgcgctggc gcttgcgccg gacggaacgc ggttgatcgt ggcaactggc
     2221 gaggcgtccg gtcagatcga ggtctgcgac gcgcgttcgg gacgcgtcga gcgattggtg
     2281 cgcgccggcc acaccccgtc cgcgctcgcg gtatcaccgg atggcttgcg gctctacgtc
     2341 gcgaaccggt tcaaccacga cgtcagcgtg atcgactgga agggggggcg ggagcttggg
     2401 cgcattccgg tcgtgcggga gcctgttgcc ctggtcctga cgccggatgg gcggcgtttg
     2461 tttgtgggca atcaccttcc cgcgggccgg gcggacgcgg cggaaattgc ggcggcgatc
     2521 accgtgatcg acacggaacg gcttgaaccg gcggcccaga ttcagcttcc aaatggcagc
     2581 acggcggtgc gcggtctggc gatgtcgggc gacggcgaga ttgtgtacgc gacgcacggg
     2641 cttgggcgct accagatgcc cacaacgcag ttggaccgcg gctggatgaa cacctcggca
     2701 ttgagtgtga tcgatgcggc caaggcggaa cgcatcaaca cggtgttggt ggacgacgtg
     2761 gacctgggcg ctgcgaatcc atggggcgtg gcctgttcga gcgacgggcg ctggattgtc
     2821 gtcagccacg ccggcacgca cgaggtgagc atcatcgacc agcccggtct gatcgagaag
     2881 ctgcgtcgga ttggggcggg tgaacgggtt gatggggcga gtctttcgtc cgaggatgtg
     2941 ccgaacgatc tgagtttcct ggtcgggttg cggcgccggg tgaatctgag tggggagggg
     3001 ccgcgggggg tggttttggt gggcgagcgt ttgttcgttg ccgagtacta ctcggacagt
     3061 ctgggtctgt tggatttggc caccgccggg ccgctgcggg ccaagtcgtt tccgctcggt
     3121 ccggcagtgg cgccgacgca ggcgcggcgc ggcgagatgc tcttcaacga tgcgcggctc
     3181 tgttttcagc actggcagag ctgcgccagt tgccacccgg acgcccgggt ggacgggttg
     3241 aactgggacc ttctcaacga cgggctgggc aatccgaaaa acaccaagaa catgctgctc
     3301 agccacgaga cgccgccagc gatgatcatg ggtgtgcgcg agacggccga ggatgcggtg
     3361 cgcgccggca ttcgtcacat tcagtttctg gtgcgacccg aggaagaggc ggttgccatc
     3421 gacgtctacc tcaagaatct ccgacccggc ctcagccccc atcggcaagg caaggacttg
     3481 agcgcggcgg cgcagcgggg ccagcagatt ttccagcgcg ccgggtgcgc cgagtgccat
     3541 ccgccgcccc tgtacaccga cttgcgacgt cacgatgtcg gcacggggac cgggcgtgaa
     3601 caggggaagt cgatggacac gccgacgctg atcgagtgtt ggcggaccgc gccgtatctg
     3661 catgacgggc gggccgccac catgcgcgac gtgctgggcg aggcgaatcc ggacgatcgt
     3721 cacgggcgga cctcgggcct gacgccgatc gagttgagcg atttggccga gtacgtgctt
     3781 tcgctctgag cggatgcgtt ttctgagcgc gtgcccaaga caggccgctg atgtcgacga
     3841 ggcgcgaccg ggggtggtcg cgccccggta tgggcatcag gcgccgaact tgtcgaacat
     3901 ccgcgcgttg gcctgcatga ggcggatgag gtacttggct ttgaagacgc cgagcgatcc
     3961 catgttcgag cccgtctcgg tgaagcgctc gagtttgtct gatccgctca acgcggagtg
     4021 cacgaggacc ggctgggggt gccacgagtg gcccttgacc gcgcacggcg tcgagtgatc
     4081 gccggtgatg gcgatgacgt caggcttttt gcggaggagg atcgggaggg cggcgtcgaa
     4141 atcctcaatc gccttctttt tggcttcgaa gttgccgtct tctccggcct tgtcggtgta
     4201 cttgtagtgg atgaagaaga agtcgtagtt gtcgtattcc gcgaggtaac gctcgaattg
     4261 ttcggcgatg gtcttgggcc cttcgatctt ggtcatgccg accaactggg cgagtccctt
     4321 gtacatcggg tagaccgcca gacaggccgc cttgagttgg tagcggtcct ggaagagcgg
     4381 aatccggggc tgatgggcga tgccacgcat caggaacccg ttggcccgcg gatgcttggc
     4441 gagcagcggc aacgcccgct tgtagaagtc cgccacgatt ttcgccgtct tcacctgcgc
     4501 caccgatttc tcgttctcgg gcttagcgat ggcgatcggc tggccttccc ggttcggatc
     4561 ggtcgatgac agcgggccgg ccaggccttc gccgcggaag acgacgacga accggtgttc
     4621 cttgccgggc tggatgatga cctcgacttc gctgcctttc gggccggccc tcttgattcc
     4681 ccgcgaaagg ataccgcaga gttcctcgca cacgtcggtg gaaattcggc ccgcgcggcg
     4741 gtcgatgatg atccccttgg gatcgagaga gcagaaattg gcgcgggcgg ccacatcgcc
     4801 ggctttgagt tgcacgccca ggcccagggc ctcgataacg ccgcgtccga cctggtactc
     4861 gatcgggtca taaccgaaca gggccaggtg gcccgggccg ctgccaggcg tgatgccctg
     4921 cgccacgggt atcatgcggc cctgggcgca gccttgggcg acaaaggcgt cgagattggg
     4981 cgtgcgcgcg gcttcgagcg gcgtgatttc gccctggccg acgatcgcga gatcgcccag
     5041 gccgtccagg acgatcaagg cgagtttggc cttggtcggg atggtcagtt ccgagtagag
     5101 agagtcgagg ttcataggta tttcagtttt caggttcaag gttttctccg gtggccgggg
     5161 cgtctccgat tgccggcccg cccgaccaga acgcggatag tgggtgcgac gcccgcccag
     5221 cgtcaacgcg atattcggat ctcctccagt ttggcccagg cccgtccgct atcaatcaga
     5281 tcggcggccg cctcccagcc gtcggcgacg gtgcggaccc gatccgccac gaacaacgcc
     5341 gcgccggcgt tgagcagcac ggcgtcgcgt ttgggaccgc gctcgtctcc gcgcagcacg
     5401 cgccgcaagg tctcggcatt ggtggcgcgg tcgccgccct gaaggtccgt cagaatggcg
     5461 ggctgcatcg ggaaatccgc ccgtctgagc gtgctgcaat gcacgccacg ttcctggtag
     5521 aactcagcaa tcgtggtgtc gccgagaacg gacaactcgt cgaagtacat ggcgcctgtc
     5581 gccaggccgg tgcggccgca gacgacgatg ccgcgttgga cgcccaggga ctggaggacc
     5641 atggccaggg gctcgcacag atcgggccgc ggcacgccca tcacctgcgc cgtcggacaa
     5701 accgggttga gcagcgggcc cagatagttg aacaaggttc gctggccgcg gtcggcacag
     5761 agccgccgga cgggggcgat gtgtctaaag gccgggtgat acaaaggggc gaagaagaag
     5821 gcgaaaccgt ggtcgcgcaa agccgagacg gcccgttcgg gcggcaggtc gatggggatg
     5881 ccgagcgctt cgatgacatc ggcgctgccg gtctggcagg tcacggcacg gttgccgtgt
     5941 ttggccacct tcaccccggc ggaggccgcg acgagtgcgg cggcggtcga gatgttgaac
     6001 gtgccggccc ggtcgcctcc ggttccaacc acgtccaaga ttccgccgtt ccgcaaagtc
     6061 gtgtccaggg gaggcgcgat cgccttgccg cggaggaatc gcgcaaacgc ggcgatttcg
     6121 tcggctgtct cccctttccg ggccagggcg ctaaggaaat cggcctttgt ctcgatcgac
     6181 aacgattcgt cgaggaggca agcggcggct gcggcggctt catcgccagg catggccagc
     6241 cccgatcgca cgtgcgcagt gagtctttcg agcacgggcg cagggtagcg acttccgacc
     6301 gggaacgcca ctcgttcgtt taagggtctg tgcaggctca aggagaaggc cccgcggcgg
     6361 gcagccacgg ggcctgtcga atccgattca gagaagtccg gatcaggagg gcttcagggt
     6421 gccgtaagtg acgggatcga cctttttgcc ggtgccatcc atgacggtcc gggtcttctc
     6481 gtcgtagtgg caggcgatgc ccaggcgatc cgacatctcg ccgagcgaga caatggcctg
     6541 gacgcgcacg gcgaggtcga tgttcgcgtt gggttgtttg ccggaccgga tgcaggatat
     6601 ccagttcgat tcatgctcgc cgatgtcctc gacggggagg agattcttga agctttccgg
     6661 atcgatctcc tcgctgaact tggcttccgg cttgaggtcc acattggccc cgccgatgag
     6721 cagcgtggca tagtggccgc ggatgacgtc gggcagcccg tattcgttga ccgaactgct
     6781 gcagaccatg aggctgtagc cgctcgggaa ctcggccacc agggtgagac actcggggca
     6841 atctcgctcc tgcgtgccgg gcgtgttctt gtccgtgtga atcgccttgg tgcccacggc
     6901 gacaacccgg ttcgggaact cgggattgcc ggtggcgagc acaagcgggt ggagccgatg
     6961 ggggaagaga tcgcccagga gaccggcgca gtatggatag tacttgcgcc agcggaagaa
     7021 atgatccgcg ctgaagtcgg ctcgggtctt gatgttgggg ccgagccact ggtcccactg
     7081 aacgctgccg gccttcaggt cgggatcgat tgtgtaattc cactccccgg cggggctgtt
     7141 gcgcatgtac gagccctgac caagcaccag cggccccaga gaccccgctt tgatcaactc
     7201 ggcgcacttg tgccacttgg cgtccgaaca gccttgggac ccgacctgga agaccttgcc
     7261 ggtggccttg accgtgtccc agacctcgaa ggcctccggg agatagcggc tcatcggctt
     7321 ctcgacgtag atgtgtttgc cggctttcat cgagtcgatg gagatttgcg cgtgccagtg
     7381 gtcgacggtc gcgcacaaca cggcatcgat gtctttgttt tcaagcatct tacgatagtc
     7441 gtcgtaagtc ttgcagtcgc ccatgaccgc cttcgccgcc tcgcgccgct tctcccacag
     7501 atcgcagacc gccgccaacg ccacattgtt cttgtcggca ttctgcttca tcttgttgac
     7561 gtgcagcatg ccctgcggac cgacgccgac gaagccgacc gcgatccgat cgttggcgcc
     7621 gatgacccgt ccggtggacg gcgcttgggt ttgcccgtaa accggggtct tgaacatatt
     7681 cactgccgcc acagccgccg tggccgtcgc ggtcctgcga atgaaactcc ggcggctttc
     7741 cccgccggcc gattgctgag ctaagtcgtg ttgcataggc aaatggaata ggatttacgt
     7801 tgccgttatg gacgcagcct agggcttttc tcttcacggt caatccgaag ttgcgtcgag
     7861 caaccgcgca tccgagacct cgatgaacgt tcactgcaac catttgatgg acaccgagca
     7921 tgcgcgcctg ggcgccggca gttgacgcgc gcggagcaac gccttccggc atcccccagg
     7981 gtatcggcac atctccttga acatgctgtg tgcccgcagg gcgcggtcca cgcccccggg
     8041 tccgagcacg cgcgctgtga gcctccccgc ataccccgat gccaaaccca cgcaatccat
     8101 tggaaaggaa ggaaagtcgc aaagagcgca aagctcttca tgcatccctc ctgcgcgccg
     8161 ttgctatgct ccccactgtc cttccattgt gacgcgaatc ccgcactcgc cgaatctgcg
     8221 agtctagaat cacttcctgc accggctctc ggcgtattcc agtttgccca cctcggctgc
     8281 cgtcaatgcc gcgcgcagtg gtttgagggc tttgacgaca gtcgcatagt ctctccgacg
     8341 ccatgccgcg tcgggattgg gccgggcggc tcgcaagcgt tctctaaggg cgtaagtccg
     8401 agcttctttg cggcggcaat tggccagctc agcaaagctc gtgctgtttc ctgtgagcaa
     8461 gtcgctccca tatgtgcgga gcgcgtcggc gagcttctga acccaccttg ccattgccag
     8521 actagtcgtg atctggatca gcgagaaaga ggctgcgtcg ggagctctgc gaaaccgcag
     8581 aatctcgtcg atcgaaaaag gtggggagta ggcatctctt ttgccaacga gcagcccgag
     8641 ctcgaaggat cgctctccat caaagaccag ttcgacaaac acagtgcgag actcgaatcg
     8701 catccgacat ggagttgatt cggtgcatgt atagcctctt tcgcgtagca gaaatccgaa
     8761 gctccgttcc gcgtgtgcct tcaggccaag atctggcata gctcaatctt cgttcggtat
     8821 ccgcccccaa cgcctctccc tttgctgtcg gttattcctc cccctttgct cgggatccgc
     8881 gttcgactga ggtgcgtgcc cctgtcatca tatcaatcgc gcccgctttg cagccctcga
     8941 tcaatgcgtc cggatccaac ccggtcgcgg cgataaccca cccggcacgg tcgtgacaga
     9001 tcacctcatg gacaccggca gagacctcgg tcacatcgaa gatccatccc ctagtttgcg
     9061 gaaatactcc ggttgcgggg ggccccacag tcgcttcatt cttcggtttg ttcatccctg
     9121 atccctcgga cacatgtcgt cgaatcctgt cccgccatca atgcctcagc atcttcagtg
     9181 ccaaaacccg aggtctgtgt tctcaactca acttgcggtt ttcccgcctg aggcctcgcc
     9241 caatcaggat ctttcgccag aatctcttgg agtctccgga aggcagccac agactgttcg
     9301 caatcctccc gtaccacgac atacaagtcc attctgtacc cctccatgta gcgcacgacc
     9361 ttctcggtga cccgtacgat atcgtcaaca gaaagagaag ttgatttccc atccacgcac
     9421 catccgtcat cagggcacag tgacattgtg aatccttcct tgagtgacct actatcaata
     9481 tgcaccctga cgatacgatc cttgtagatg agcttgcttt ctttccaggc atagagcacg
     9541 ccgtaccgcc ctctgcacaa gcgcacgaca atgtgccgga tgcgccataa cagcgaacgc
     9601 cgtcgtgtga ttaagaagtg cgcacctcgc aggggcgcct cccctctagc acagtgataa
     9661 taatccgtat tcacttttca gcctcgtcgc cagaatctgt gggtcagaat gctgagcatt
     9721 cagacgcaac atattcacca ggagtatctt gccacacaaa acgcggcaga gtgcattagt
     9781 acctacagat tctgccacaa aaccactctt cataaacact agcctggatt tccacggacc
     9841 ggaattgagc taagttttcg aggtttta
//
LOCUS       DAOQRO010000097         9851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6210_length_9851_cov_5.114537, whole genome shotgun sequence.
ACCESSION   DAOQRO010000097 DAOQRO010000000
VERSION     DAOQRO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6210_length_9851_cov_5.114537"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKK20_05285"
     CDS             <1..256
                     /locus_tag="PKK20_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNU99330.1"
                     /translation="LMAGDFNARPDSRVMQHLLDPSTGWMDTAGAWAAPTSPSESPRS
                     RIDYVLASPRTAWETVQSEVISEAAASDHRPLSVVLRLHK"
     gene            278..1198
                     /locus_tag="PKK20_05290"
     CDS             278..1198
                     /locus_tag="PKK20_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNU99331.1"
                     /translation="MRGSNRISSWPGAAALLGAALGVWICTTNAASSEVYIVRKNDSL
                     SQIAQTHGVRVQELARVNGLSLESTIYPGQRLQIPLPARDQERHDARTVTVRKNDSLT
                     LIARRHRVSVAALARANGLSLDDLIRPGDKLRLPTAAPARPALEMRVQKAIDIAPVRR
                     GMWKQIVIHHTATTMATIRGMDEYHRMECRMENGLAYHFVIGNGQGIPDGRIEVGNRW
                     TRQLEGGHLSTRSQNLVSLGVCLVGHFDRHRPTPKQLESLDALLRALMKRCGLGPSAI
                     TTHQLVSKQRTRCPGKFFDLKLVLAELRKD"
     gene            1259..1504
                     /locus_tag="PKK20_05295"
     CDS             1259..1504
                     /locus_tag="PKK20_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNU99332.1"
                     /translation="MPLYEYELCEGDCAVCGGRFTLRRPVTAKPLTQCPACKKPVRRV
                     ISSFNTPKLLKPLSVTDAKKAGFTVLKRTGKGEYERQ"
     gene            1575..3512
                     /locus_tag="PKK20_05300"
     CDS             1575..3512
                     /locus_tag="PKK20_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99333.1"
                     /translation="MSALQLIEATVIRTGRRRRWHRAWRGAWQGFLIAAVLWLVALGL
                     YKLLPIPWQVLPLAGVTGLLTVLAGFLAGWWRRERAIDTARWLDHRQHLQERLGTALE
                     IGREPGSGEWGALVLNDAARMVGEVDVRRLLPLTLPRASRWSLPILALAAGLGFAPEY
                     RSQEFLQRQREKESIRETGRNLSELTKRSLERRQPTLEATRQAVKSVGELGEQLARNP
                     VSRTEALRDLAKMTDRVEQEHRELGKNPALRAIERAARSADKNSPASSDALQKQIESM
                     QKALGSKEAAADALEKLRRELDKARQAAANMLGKDSQANAAAQESLANALSSLARQST
                     DLGSALPSLEEALAALKAGDIDQVLKNLEVAEKNLERLLEMAQALDKLQQQAGKLGKD
                     LAEQLEKGQAEAAIASLQKMVNQLKAGDLSPERMKALMDEVAKAIDPAGPYGKVPDHL
                     KEALRQMQAGQNAPAAQALADAAKELESLLDQLADAQDLKATLDALKRAQICVGTCQS
                     WGACQGLSKPGFKPGGKPGRGVGTWADEDGWLDTPPTTGLWDNSGIERPDMESRGITD
                     RGEGEIPEGMMPTKVKGQFQPGSQMPSITLKGVSIKGQSRVAIQEALQAAQSEAESAL
                     SHDKVPRAYAGSVKDYFDDLK"
     gene            3509..4510
                     /locus_tag="PKK20_05305"
     CDS             3509..4510
                     /locus_tag="PKK20_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNU99334.1"
                     /translation="MSTQEQITHFRTIFSALRDEVGKIIVGHESIVDNTLVAILAGGH
                     VLLEGVPGLGKTLLVRTLGEVLDLSFSRIQFTPDLMPADILGTNLVMETPDGRRLFEF
                     QKGPIFAHLILADEINRATPKTQSAMLEAMQEHSVTAGGALRRLTEPFFVLATQNPID
                     QEGTYPLPEAQLDRFFFKLLVGYPTAAELAEVINRTTETAKTELRRVVSREQLLEMQL
                     LVREVPVASHVKDYAVRLVQATHPKTETGTAMVSQFLRFGSSPRGAQALILAGKVRAL
                     AQGRFNVSFDDLEAIALPALRHRLILNFEAEAEGVTTDHIIAQVLKDVPRDAVAVGA"
     gene            4530..5426
                     /locus_tag="PKK20_05310"
     CDS             4530..5426
                     /locus_tag="PKK20_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNU99335.1"
                     /translation="MTQSLLSPELLRRLERVQLLAARRAKSSARGERRSRSRGQSVEF
                     ADYRNYSVGDDLRYLDWNLYGRLDRLFLKLYEEERELPVRLFLDASESMTFGDPVKFD
                     FARQVAAAIGYVALCGFDRVSVLVFPDHPEDVVARGALRSVRGRHSAFALFNHLGALK
                     AGGTAQLNGALHRGAVEARQPGLAVVLSDFLDPDGYEAGIGALLGRGFQVDCVHILAP
                     DELSPTTFGDLRLIDAETGGHQEVTFGRYRLAAYRDTVSRFCRRLREFCAARGVNYFL
                     ASSATPLEDLLLRQLRKAEVLG"
     gene            5437..7272
                     /locus_tag="PKK20_05315"
     CDS             5437..7272
                     /locus_tag="PKK20_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNU99336.1"
                     /translation="MSFLTPAAFAFAAAIPVVILFYLLKRKRVVKLVSSTLLWQKYLA
                     ETQANAPFQKLRHNWLLVLQILLLLALIFALARPYRAGQVSGGRLFVAIIDASASMQS
                     TDETPSRFERARREALELVDSLHDTDQMVVLQAAGHTEVKQSPTGNKSALRRALQGCA
                     VTDAPTRLAEALKLAQPLVKDRIDAEIHLFSDGAAPDLTEFEHEGLNVVYHRLGVRAD
                     NVGLVGLDVRAHPEEPARRALFASVANASTNPVQTELQVLLDDQLIETRLLELRPRET
                     LPQVFAATQTKDGIFTARLVASDDLLADNQASLVSLLPAPVRVLLVTRGNRFLERALA
                     AAPGVQLSVADDLVEGSEDFDVTVLDDVLPAVWPAGNTLAIRSANTNWVEVTGRVETP
                     TIVDWRTTHPLLRFVNFDNVQLAEALTVKPSAWAVSLVDSSASPLILAGEAGRQRFIW
                     IGFDTLQTTWPLRISFPIFIANAVAWLDPAATKADQFQVRAGDPIRLALTESLTNATI
                     RLPDGSERPIAIDPGARELVFGQTALQGIYRIEAGTNRFVVSANLLDGAETDTTPKPE
                     LKFGKYARVEATSTKRASLEWWRWLAASALAVLGFEWWFYHRRTA"
     gene            7538..>9851
                     /locus_tag="PKK20_05320"
     CDS             7538..>9851
                     /locus_tag="PKK20_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNU99337.1"
                     /translation="MNWSLFQNSLLVSTGAALAALLWGVTAALFVSGLGSRWRAIVLA
                     LAVVAIALPPFLVTNTWIHLLGQTGAWRPWLPLNIYSPGGAVWTLSLLLWPVPLFFTL
                     AAWAQIPPSQVESDGALTGVALFRWILVPSARTPLFEALMITWVLALGNFSVPAILQI
                     KVFPAELWIRFSTNLDFWGAALLCLPLILPPAALVLWLRRRPLRWPRLEPALAAALAR
                     RRLGRWWWGSAGISSLLLILGVCLPLVELANSPRTWSELPGALAAGGGALWASFHVAA
                     LTATLATGLGLAAWRWSAGQPLWILWLTPGVFLGVVCIFLFNRRPLTPFYQSSGLVIL
                     ALTLRCAAIGWQGARWTRQTLDPDLDDAARLDGANRWELFRRVRWPQIAVPLAALWYV
                     IYLLTLWDVETVLLIVPPGGETLALRIFNLLHYGHNAQVDALCLILLALALAPLAAWQ
                     AARICGLARAERIPIGAVLAGLLAGCSQHAPGHAPLDSELFDRVEVWGTRGVGPGQFS
                     KPRSVTVDRADNVYVVDMTGRVQKFSPLGEWLLAWQMPETDLGKAKGMGIDRDGRVIV
                     VEPHYSRVNHFSAEGRIEQQWGTHGTNAGQLAFPRAVAVNSRGELWVSEYGIVERVQR
                     FTPDGASRLAGFGRYGPTPGEFNRPEGLFVDAQDRLFVADSCNHRVQVFSDNGQWLAE
                     LGHAGAGPGAMSYPYDVAVDRAGNLFVCEFGNSRIQVFDAHHRTREIIGRAGAAPGEF
                     NNPWGIALDSKGNLYVADAGNHLIQKFIRRP"
BASE COUNT         1698 a         3348 c         3133 g         1672 t
ORIGIN      
        1 actcatggcg ggcgacttca acgcgcgccc cgacagccgc gtgatgcaac acctgctcga
       61 tccttcgaca gggtggatgg atacggccgg ggcatgggcc gcgccgacga gtccgtccga
      121 atcgccacgg tcgaggatcg actacgtgct ggcttcaccc cgcacggcct gggaaaccgt
      181 ccaatccgaa gtgatttccg aagcggcggc ctcggaccat cgtccgttgt cggtagtgct
      241 ccgtctgcac aagtaacctc gatcgaacgc ccggaccatg cgcggttcga atcgcatttc
      301 gagttggccc ggcgcggcgg cgctcctcgg tgcggcgctc ggggtctgga tttgcacgac
      361 aaacgccgcc tcatccgagg tctacatcgt caggaagaac gattcgctca gccagatcgc
      421 ccagacacac ggggttcgcg ttcaggaact cgctcgggtc aacggcctct cgctcgaaag
      481 cacgatctac cccggccaac gtctccagat cccgctgccg gctcgggacc aggaacggca
      541 cgacgccagg actgtgaccg tgcgaaagaa cgactcgctc acgctcatcg cccgtcggca
      601 tcgcgtcagc gtggcggccc tcgcccgggc caacgggctc tccctcgacg atctgatccg
      661 tcccggagac aagctccgcc tgccgacagc cgcgccagcc cggcccgcgc tcgagatgcg
      721 cgtccaaaag gcaatcgaca tcgcccctgt gcgccgcggg atgtggaagc agatcgttat
      781 acaccatacg gccaccacca tggccacgat ccgcggcatg gacgaatacc atcgcatgga
      841 atgcaggatg gaaaacggcc tggcgtatca cttcgtcatt ggcaacggcc aaggcatccc
      901 cgacggacga atcgaggtcg gcaaccgctg gaccaggcaa ctcgagggcg gacatctctc
      961 gacccgttcc cagaatctgg tcagcctggg cgtctgcctt gtcgggcact tcgatcgcca
     1021 tcggcccacg ccaaaacagt tggagagtct cgacgccctg ttgcgcgccc tgatgaaacg
     1081 ctgcgggctg ggcccgtccg ccatcaccac gcaccaactg gtcagcaaac aacgcacgcg
     1141 ctgcccgggc aaattcttcg atctcaagct ggtgctcgcc gaactgcgga aggattgaaa
     1201 tccggccccg gcttgatccc agggccggac gggcgtagac tgtcccgcgt tcgaaacgat
     1261 gccgctctac gagtacgaac tttgtgaagg ggattgcgcg gtgtgcggcg ggcggttcac
     1321 gctgcgccgt ccggtcaccg ccaagccgct cacccaatgt ccggcctgca agaaaccggt
     1381 gcgccgagtg atttcgagtt tcaacacgcc caagctgctc aaaccgctct ccgtcaccga
     1441 cgccaagaag gccgggttca cggttctgaa gcgaacgggc aaaggcgagt acgaacgcca
     1501 gtgatggcca cagcgaaagc gatgccaaaa gggctcgcca aggatcgagt gaacctgcac
     1561 cttaagccgt tctcatgagc gcactgcaat tgattgaggc gacggtgatc cgcacggggc
     1621 gtcggcgccg gtggcaccgg gcctggaggg gcgcgtggca aggctttctg atcgccgccg
     1681 ttctttggct ggtggcgctc gggctctaca agttgctgcc gatcccgtgg caggtgctgc
     1741 ctctggccgg cgtcacgggc cttctgaccg tgttggccgg attcctcgcc ggctggtggc
     1801 gccgcgaacg cgccatcgat acggcccgct ggctcgacca tcgccagcac ctccaggaac
     1861 ggctcggcac cgcgctcgaa atcggacgcg agcccgggtc cggcgagtgg ggcgcgctcg
     1921 tgttgaacga cgctgcccgc atggtgggcg aggtggatgt gcgtcggctg ctgccgctca
     1981 ccctgccccg tgccagccgg tggtctctgc ccatcctcgc cctggcagcg ggactcggtt
     2041 tcgctccgga ataccgcagc caggagtttc tccagcggca gcgcgagaag gagagcatcc
     2101 gcgagaccgg gcgcaacctg tccgaactga ccaagcgcag tctcgaacgc cggcagccga
     2161 cactcgaagc gacgcgccag gccgtcaaat cggtggggga attgggcgag caactggccc
     2221 gcaacccggt gagccgcacc gaggccttgc gcgatttggc gaagatgacc gatcgcgtcg
     2281 agcaggagca tcgtgagttg gggaagaacc ccgccttgcg cgcgatcgag cgcgcggcgc
     2341 gcagcgcgga caagaacagc ccggcatctt cggacgcgtt gcagaagcag atcgagtcga
     2401 tgcagaaggc gctggggagc aaggaagccg ccgccgatgc gctcgaaaag ctccgacgcg
     2461 agctggacaa ggcccggcag gccgccgcca acatgcttgg caaagactcg caggcgaacg
     2521 ccgccgccca ggaaagcctg gcgaacgcat tgtcgagcct ggcgcgccaa tcgacggacc
     2581 tgggaagcgc gctgcccagt ctcgaggagg cgctggccgc gttgaaggcg ggcgatatcg
     2641 accaggtgtt aaagaacctc gaagtcgccg agaagaatct cgagaggctg cttgaaatgg
     2701 cccaggcgct cgacaagctc cagcagcagg ccggcaaact cggcaaggac ctcgccgagc
     2761 aactcgagaa gggccaggcc gaggccgcca tcgcgtctct gcagaaaatg gtcaaccaat
     2821 tgaaggcggg cgatctctcg cccgagcgca tgaaggccct catggacgaa gtcgcaaagg
     2881 ccatcgaccc agcgggcccg tacgggaagg ttccggacca tttgaaagag gcgctgcgcc
     2941 agatgcaggc gggtcagaac gcgcccgcgg cccaagccct ggcggacgca gccaaggaac
     3001 tcgaaagtct gctcgatcaa ctggcggacg cgcaggacct caaggcgact ctcgatgccc
     3061 tcaagcgcgc ccaaatctgc gtcggcacct gccagagctg gggcgcctgc caaggcctga
     3121 gcaaacccgg ctttaagccc ggtggcaaac ccggccgggg cgtcggcacc tgggccgacg
     3181 aagacggttg gctcgacacg ccgcccacca cgggtctgtg ggataattcc gggatcgagc
     3241 ggccggatat ggaatcgcgg ggaatcaccg accgcggcga aggcgagatt cccgagggga
     3301 tgatgccgac caaggtcaaa ggccagttcc agccggggag ccagatgccg agcattacgc
     3361 tcaaaggggt cagcatcaag ggacagagcc gtgtggcgat ccaggaggcg ttgcaggcgg
     3421 cgcagagcga agccgagagc gccctgagtc atgacaaggt cccgcgcgcc tatgccggga
     3481 gcgtgaagga ctacttcgac gatctcaaat gagcacccaa gaacagatca cgcatttccg
     3541 cacgatcttc agcgcactcc gggacgaggt gggcaagatc atcgtcggcc acgagtcgat
     3601 cgtcgacaac accctcgtcg cgatcctggc cggcgggcac gtccttctcg agggggtgcc
     3661 gggtttgggc aagacgctgt tggtgcgcac cctgggcgag gtgttggacc tctcgttcag
     3721 ccgcatccag ttcacgcccg acctgatgcc cgccgacatt ctcggcacca acctggtgat
     3781 ggagacgccc gacggccggc ggctgttcga gttccagaag gggccgatct tcgcccacct
     3841 gatcctggcc gatgaaatca accgggcgac gcccaagacg cagtcggcca tgctcgaagc
     3901 gatgcaggag cacagcgtga cggcaggcgg ggccttgcgc cggctcaccg agccgttctt
     3961 cgtgctcgcg acgcagaacc cgatcgatca ggaagggacc tacccgctgc ccgaggccca
     4021 actcgaccgg ttcttcttca agctcctggt cgggtacccg accgcggcgg aactggcgga
     4081 ggtgatcaac cggacgaccg agaccgccaa gaccgagctg cgccgcgttg tgtcgcgcga
     4141 acaactgctc gaaatgcagc tattggtccg ggaggtcccg gtggcctcgc atgtcaagga
     4201 ctacgccgtc cggctcgtcc aagccacgca tccgaagacc gagacgggca cggccatggt
     4261 ctcccagttc ctgcgcttcg gcagcagccc gcgcggggct caggcactga tcctggccgg
     4321 caaagtccgg gcgctggccc agggacgatt caatgtcagc ttcgatgacc tcgaagcgat
     4381 cgccttgccg gcgctccgcc atcgcctcat tctgaatttc gaagccgagg ccgaaggtgt
     4441 caccaccgat catatcatcg cccaggtcct gaaggacgtg ccgcgggatg cggtggcggt
     4501 cggggcgtga gttgcccgtc gaggcccgtg tgacacagag tcttctcagt cctgaactgc
     4561 tccggcggct tgaacgggtc cagttgctcg cggcgcggcg ggcgaaaagt tccgcgcgtg
     4621 gcgaacgccg cagccgatcg cgcggccaat cagtcgaatt tgccgactac cgaaattact
     4681 cggtcggcga cgacctgcgc tacctggact ggaacctcta cggccggctc gatcgcctct
     4741 tcctcaaact ctacgaggag gaacgcgaac tgccggtccg cctgttcctc gacgccagcg
     4801 aatcgatgac cttcggcgac ccggtgaaat tcgacttcgc ccgccaggtg gcggcggcga
     4861 tcggttatgt ggccttgtgc ggcttcgacc gcgtctccgt gcttgttttt cccgaccacc
     4921 ccgaggacgt ggtcgcccgc ggcgcgctgc ggtcggtgcg cggacgccac tcggcgtttg
     4981 cgctgttcaa ccatctcggg gcgctcaagg cgggcggaac ggcccagctt aacggtgcgt
     5041 tgcaccgcgg tgccgtcgaa gcccgccagc ccggcctggc cgtggtgctg agcgatttcc
     5101 tggacccgga cggttacgag gcgggcatcg gggccctgtt gggacgcgga ttccaggtcg
     5161 attgcgtgca catcctcgcg cccgacgagc tgagcccgac cacattcggc gatcttcgat
     5221 tgatcgatgc cgagaccggc ggacaccagg aagtcacgtt cggacgctac cgtctggcgg
     5281 cctatcggga caccgtatcc aggttttgcc ggcgtttgcg cgagttctgc gccgcgcggg
     5341 gtgtgaacta cttccttgcg agcagcgcca cgccgctcga agacctcttg ttgaggcaac
     5401 tgcgcaaggc cgaggtgctc ggatagccaa aaccccatga gcttcctcac ccccgccgcc
     5461 ttcgcattcg ccgccgccat cccggtggtg attcttttct atctcctcaa gcgcaagcgc
     5521 gtggtcaaac tcgtctcgag cacgctgctg tggcagaagt acctcgccga aactcaggcc
     5581 aacgccccct tccaaaagct gcgtcacaac tggctcctcg tcctgcaaat cctcctgctc
     5641 ctggctctga tcttcgccct ggcgcgtccg tatcgcgccg gacaggtctc gggcggacgg
     5701 ctgttcgtgg caattatcga cgcctccgcc tcgatgcaaa gcaccgacga aacgccgtcc
     5761 cgattcgaac gcgcgcggcg tgaggcgctc gaactggtcg acagcctcca tgacaccgac
     5821 cagatggtcg tgctgcaggc cgccggccac accgaggtga aacagtcgcc cacaggcaac
     5881 aagagcgccc tgcgccgggc cctgcaaggc tgcgcggtga ccgacgcccc cacacgcctg
     5941 gccgaggccc tcaagctggc ccagccgctg gtcaaggaca ggatcgatgc cgagattcac
     6001 ctgttcagcg acggtgcggc gccggatttg acggagttcg agcacgaggg actcaatgtc
     6061 gtctaccacc gcctcggcgt gcgggccgac aacgtcggcc tcgtggggct cgacgtgcgc
     6121 gcccacccgg aagaacccgc ccgccgcgcc ctgtttgcca gcgtcgccaa cgcctcgacc
     6181 aaccccgttc aaaccgaact gcaagtcctg ctcgatgacc agttgatcga aacccgcctg
     6241 ctcgaactcc gcccgcgcga aaccctgccc caagtcttcg cggcgacaca gaccaaggac
     6301 ggcatcttca ccgcccgcct cgtggcttcg gacgacctcc tggccgacaa ccaggcctcc
     6361 ctggtcagcc tcctgcccgc acccgtgcgc gtgctgctgg tcacccgggg caatcgcttc
     6421 ctcgaacggg ccctggcagc cgcgcccggc gtccagctca gtgtggcgga cgacctggtc
     6481 gagggttcgg aggatttcga cgtgacggtg ctcgacgatg tgctgccggc ggtctggcct
     6541 gccggcaaca ccctggccat ccgctcggcc aacaccaact gggtcgaagt caccgggcgc
     6601 gtcgagaccc cgaccatcgt cgactggcgc acgacgcatc ccctgctccg ctttgtgaac
     6661 ttcgacaacg tgcaattggc cgaagccctc accgtcaagc cgtcggcctg ggccgtgtcg
     6721 ctagtcgatt cgtcggcctc accgctgatc ctggccgggg aagccgggcg ccaacggttc
     6781 atctggatcg ggttcgacac cctccaaacc acctggcccc tgcgcatctc gttccccatc
     6841 ttcatcgcca acgccgtcgc gtggctcgat ccggccgcca ccaaggccga ccaattccag
     6901 gtgcgggcag gcgatcccat ccgcctcgca ctcaccgaat cgttgaccaa cgcgaccatc
     6961 cggttgccag acggttccga acgccccatc gccatcgacc ccggagcgcg agagttggtg
     7021 ttcggccaga cagcgctcca gggtatctat cgcatcgaag cggggacaaa tcgtttcgtg
     7081 gtttccgcaa acctgctcga tggcgccgag acggatacga cgcccaagcc cgagttgaag
     7141 tttgggaagt acgcgcgggt tgaagcgact tcgaccaagc gggccagtct cgagtggtgg
     7201 cgctggctgg ctgcgtccgc cctggcggtg ctcggcttcg aatggtggtt ctatcatcgg
     7261 aggaccgcgt gatccacacc ccacgcgtcg cttccctcca cggcatctcc ccgcccgggg
     7321 agaacggcgc ggacatgtcg cgcattcgca aatccagagg aatccgtagg atacacaaca
     7381 ccggcacctt cacccgtccc gcagagcggg acaccctctt ccacctgcct gcgcccaggc
     7441 ttcggcaggc aggtcggatg ggcgagggcc ggggcgaggg ttggctgtcg cggcgtctgc
     7501 agacacgacg gtgtgtccac tcccccctgt ccgcggaatg aactggtcgt tgttccagaa
     7561 cagtctgctt gtgagcacgg gggcggccct tgcggctctg ctctggggag tgaccgcggc
     7621 tctgttcgtc tcgggcttgg gttcgcggtg gcgcgccatt gtgctggcct tggctgtcgt
     7681 ggcgattgcc ctgccgccgt ttcttgtcac caacacgtgg attcacctgc ttggccagac
     7741 gggcgcctgg cgtccatggc tcccgctgaa catctactcg ccaggcggcg ccgtctggac
     7801 cctttcgctc ttgctttggc ccgttcccct gttcttcacc ctcgccgcct gggcgcaaat
     7861 tcccccaagc caggtcgaat ccgacggcgc attgaccggc gttgcgctct ttcggtggat
     7921 tctggttccg tcggccagga cacccctgtt cgaggcgctc atgatcactt gggtcctggc
     7981 tttgggcaac ttctcggtgc cggccatcct ccagatcaaa gtgtttcccg ccgaactctg
     8041 gattcggttc agcaccaatc tcgatttttg gggagccgcc cttctgtgtc tccccctcat
     8101 cctgccccct gcggccctgg tactctggtt gcgacgccgc cccttgcgct ggccccggct
     8161 cgagcctgcg ttggcagccg ctcttgcgcg acgccgcctg gggcgctggt ggtggggcag
     8221 cgccggcatc tccagcctgc tcttgattct cggcgtctgc ctgccgttgg tcgaactcgc
     8281 caactcgccc cgcacctgga gcgaactgcc cggcgcctta gccgcgggcg gcggggcgct
     8341 ctgggcttct ttccacgtcg ccgccttgac cgccaccttg gccaccggcc tcggtttggc
     8401 ggcgtggcga tggtctgcgg gacaaccgct ctggattctg tggctgacgc ccggcgtgtt
     8461 cctcggcgtt gtctgcatct tcctcttcaa tcgccggccc ctcacgccct tctaccaaag
     8521 cagcggcctg gtcatcctgg ctctcactct ccggtgcgcc gccatcggtt ggcaaggagc
     8581 gcgctggacc cgccaaaccc tggaccccga cctcgacgac gcggcgcgtt tggatggcgc
     8641 gaaccgctgg gagctgtttc gccgggtgcg ctggccccag atcgccgtcc cgctggccgc
     8701 gctctggtac gtgatctatc tcctcacgct ctgggacgtc gaaaccgtcc tcctgatcgt
     8761 gccgcccggc ggggagacgc tggcgttgag gattttcaac ctgctgcact acggccacaa
     8821 cgcacaagtc gatgcgctct gcctgatcct gcttgccctg gctctggcgc cactggccgc
     8881 ctggcaggcg gctcgaatat gcggcctcgc cagggcagag cgaattccga ttggggcggt
     8941 cctggccggc ctgctggccg gctgctcgca gcacgctccc gggcatgcgc cactcgatag
     9001 cgaattgttc gatcgagtcg aggtctgggg cacgcgcggg gtgggtccgg gtcagttcag
     9061 caaaccacga tccgtgacgg tcgatcgggc ggacaacgtc tacgtggtcg atatgacggg
     9121 gcgggtgcag aagttctccc ctttgggcga atggctgctg gcctggcaga tgcccgagac
     9181 ggacctgggc aaggcgaagg gaatggggat agaccgcgac gggcgggtga tcgtcgtcga
     9241 gccgcattat tcgcgcgtga accatttctc ggccgaagga agaatcgagc agcagtgggg
     9301 cacgcacggc accaacgcgg gccaactcgc cttccctcga gccgtggcgg tgaacagtcg
     9361 cggcgaactc tgggtgagcg agtacggcat cgtcgagcgg gtgcaacgct tcacacccga
     9421 tggcgcgtcg cgcctggccg gattcgggcg ctacggccct acccccggcg agttcaatcg
     9481 ccccgaaggg ttgttcgtcg acgcgcagga caggctgttc gtggccgatt cgtgcaatca
     9541 ccgggtgcag gtgttctcgg acaacggaca atggctggcc gagctcggac acgccggggc
     9601 cggtccagga gccatgagct acccctacga cgtcgccgtc gaccgcgccg gcaacctctt
     9661 cgtctgcgag ttcggaaaca gccgcatcca ggtctttgac gcccatcatc gcacgcgcga
     9721 aatcatcgga cgcgccggcg ccgcgccggg cgagtttaac aatccgtggg gcatcgccct
     9781 ggactcgaag gggaatctgt acgtggccga cgcgggcaat cacctcatcc agaaattcat
     9841 ccgccgccct g
//
LOCUS       DAOQRO010000098         9845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6220_length_9845_cov_5.201021, whole genome shotgun sequence.
ACCESSION   DAOQRO010000098 DAOQRO010000000
VERSION     DAOQRO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6220_length_9845_cov_5.201021"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKK20_05325"
     CDS             complement(<1..258)
                     /locus_tag="PKK20_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99338.1"
                     /translation="MKRPAVIPVRTRMVQRASTAAKPKTAQRGKAPTEATGRPRTRGA
                     EERPGRRARSGSVWLALCLLGFGAAEPAAMTTRAGAGSPPPK"
     gene            620..1552
                     /locus_tag="PKK20_05330"
     CDS             620..1552
                     /locus_tag="PKK20_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNU99339.1"
                     /translation="MSTNSAAASSATPERAGTVNQRVSPGAVSVPLSDPSYIPGLTPA
                     IVVGPPVPLAPREPGMRFPYSTPRPARVQETVFAYDALIKETTLKAGETSAYFTFHLT
                     NTSPEAVTINAVRTSCGCAVAKLPSVPWRIEPRSGGAFDVVVDVRGSRGVTTKTVTID
                     STAGYRYLTVRVAMPESASPMAAADRARNLQVALADRQAVFKGDCAACHLQPVIAQHG
                     QALFKSACGICHEAQRRAAAVPDLRALNKPTDRAYWSGWIGKGKAGGLMPAWAIDEGG
                     PLTDRQIESLLDYLTGPFRDEPARAPLAPGAPTR"
     gene            complement(1862..2083)
                     /locus_tag="PKK20_05335"
                     /pseudo
     CDS             complement(1862..2083)
                     /locus_tag="PKK20_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2121..2678)
                     /locus_tag="PKK20_05340"
     CDS             complement(2121..2678)
                     /locus_tag="PKK20_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNU99340.1"
                     /translation="MSQTSWRLDRTATGVLVVDIQERLLPAIFERDRVVSETLCLIRG
                     ASVLGVPLFATEQYRKGLGPTVPEVAEAIPGFAPFEKLAFSACGAPGLIDAMRARAIK
                     DVIVCGIEAHVCVCQTALDLLEHGFRPFVIADAVSSRTPENRRLGLDRMRQAGAVIAS
                     NEMALFELLDKAGTDDFKAILSLVR"
     gene            complement(3032..4156)
                     /locus_tag="PKK20_05345"
     CDS             complement(3032..4156)
                     /locus_tag="PKK20_05345"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNU99341.1"
                     /translation="MNRYIPTLAACITMLLTLTPSAAATLPSGVKKSHYGTMPDGTVI
                     EQYRLTNANGIVAKIITYGALMTELHVPDRQGKLGDIVLGFDNLESYLKGHPYFGATI
                     GRVGNRIAKGKFTLDGRDYTLARNDGPNHLHGGIRGFDKVVWKAEIVPARNGAAVRFT
                     YVSPNGEEGYPGTLTATVTYTLTDTDELRLDYTAKTDQATPVNLTNHTYWNLAGGGDI
                     LNHVLTINADHYTPVDDTLIPTGAIAPVKGTVMDFTTPMPIGSRLLSLDNSPRGYDHN
                     YALNDSGKGMRLAATVYEPGTGRVLETFTEEPGVQFYSGNFLDATLEGKGKTVYHQYY
                     GFCLETQHFPDSVNHPNFPSTILRPKKTYKTATIYKFSVK"
     gene            complement(4193..4432)
                     /locus_tag="PKK20_05350"
     CDS             complement(4193..4432)
                     /locus_tag="PKK20_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99342.1"
                     /translation="MLDIRWPIGLMFTLVGAILVLTGALSSPESYQRSLGININLWWG
                     LLLLLFGGLMALFAWRAKNRPDQPTARDPGSRQNP"
     gene            complement(4461..6395)
                     /locus_tag="PKK20_05355"
     CDS             complement(4461..6395)
                     /locus_tag="PKK20_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNU99343.1"
                     /translation="MQSIQLGGIDYSILIIYSLFVLGIGFALKRFMKSSEDFFLSGRS
                     IPAWITGLAFLSANLGALELVGMAASGAKYGIATSHFYWVGAIPAMVFLAIFMMPFYY
                     GSKARSVPEYLKMRFDERTRCLNSITFAVMTLFASGISMNALAKLLHQLLGWDYNLSL
                     WICSGVVLIYVFKGGLTSAIYTEVLQFFMIVLGFAPVVYLGLKDVGGWETMTHSLSNV
                     AQDPARLGLAPGSYAPDAWSSAWKPLLGGPTANPMGVDLFAMVFGLGFVLSFGYWCTN
                     FLVVQRAMAARNMSAARRTPLIAALPKMLFPALVIVPGMIAVTLASLGKDGYRLPPKM
                     LVESAYAKAVPAVKEAASQKLDAEAAAKRIGEAAGVKMFPDRVAALLEAAPTLDDKSL
                     RNHLQNAVADTDYDGVILSLVKRYCPSGLLGLALTALLASFMSGMAGNVTAFNTVWTY
                     DLYQAYICPNKSDGHYMWMGRVVTVAGVLLSIACAYFASNYSNAMDIVQLVFGFVNAP
                     LFATFLLGMFWARTTSHGAFYGLLGGTLTSALFHSLTLAAGNNPGLKGGYLGVAQSFP
                     SEMAQNFWLASFAFSACFLLTLVISLATQRTKTNEELKGLVYSLTPKLKDEEQAWYLK
                     PTTIGWALLAGCVVLNIIFW"
     gene            6515..7429
                     /gene="argF"
                     /locus_tag="PKK20_05360"
     CDS             6515..7429
                     /gene="argF"
                     /locus_tag="PKK20_05360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNU99344.1"
                     /translation="MSIPAKHLLSLEKMDPEALRHILTATTELKRDRGRVCARPLTGQ
                     SWALIFSKSSTRTRVSFEVGIRELGGQALFLSASEIQVGRGESIKDTARVLGRMIQGA
                     VIRTYAQQDVEEFAHYSGIPTINALTDAEHPCQILADVFTFEEKRGPIAGRVVAFLGD
                     GACNVASSWIWASARLGFELRIAAPKPYQPPADLLRRAHAHVQCTDDPVEAVAGADLL
                     YTDVWVSMGKEAEASDRLSLLKDFQLNARLVKEAKPDALVMHCLPAYRGKEIDAATFE
                     ANAATIFDEAENRLHTQKAVLSWVVRPD"
     gene            7868..8005
                     /gene="rpmH"
                     /locus_tag="PKK20_05365"
     CDS             7868..8005
                     /gene="rpmH"
                     /locus_tag="PKK20_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNU99345.1"
                     /translation="MKRQYQPSKIRRKRQHGFLARNASKRGRAILARRRRVGRKRLTP
                     V"
     gene            8155..8532
                     /gene="rnpA"
                     /locus_tag="PKK20_05370"
     CDS             8155..8532
                     /gene="rnpA"
                     /locus_tag="PKK20_05370"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNU99346.1"
                     /translation="MTLPRKARLRRPGEFADVRQRGRRVVRGCLIANWVGLPGGAPSK
                     LGVVTGRKVGKAVERSRARRLLRETFRLHQHDLRAPVALVLVARPSIRGQTRMAVERD
                     FLAALRGAGLLRPAPSAVGARTP"
     gene            8529..8873
                     /gene="yidD"
                     /locus_tag="PKK20_05375"
     CDS             8529..8873
                     /gene="yidD"
                     /locus_tag="PKK20_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNU99347.1"
                     /translation="MNPAQHLAVGAIRFYRWVVSPAKTVLFGPLGRCRYNPSCSAYAL
                     EAVRTHGTLRGLWLALRRFGRCHPWGGCGDDPVPPARTPSAESAASSRLIPSASRPVT
                     ALSEALTPQGPA"
     gene            8883..>9845
                     /locus_tag="PKK20_05380"
     CDS             8883..>9845
                     /locus_tag="PKK20_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99348.1"
                     /translation="MDRKSILILVLCFVLLMLWPALVNKIFPPPKQTGSTNIVAGAAS
                     TNLFGLTNLPPSTEGPGGSGAAAALPAITAPSSGAFAWTPPTGPEETIKVESTDAIYT
                     FTSHGGGLKTVELKGTVETVPCGRGKRAASDKLATLNRDAPIPALALFAGAGLEDGRP
                     FAMRRTEGGVTVEKALTNGLRVVKEFLVQSNYMVAAAVRIENPGSEPIALPAQELVIG
                     AATPMGPYDDGTYVRLEWYDGGRTVSADRSWFSNRGLFSCLGLGGAPRPEYLSPGASN
                     VVWASLQNQFFTMVAIPQQTPHGLVARPVDLPPPSIEERERDSRI"
BASE COUNT         1652 a         3039 c         3348 g         1806 t
ORIGIN      
        1 cttgggcggc ggcgatccgg cccctgcgcg cgtggtcatg gctgcgggct ccgccgcgcc
       61 gaacccaagc aaacagaggg ccagccagac gctacccgat cgagcccgcc gacccgggcg
      121 ttcctccgca ccgcgggtgc gcgggcgccc cgtcgcttcg gtcggcgcct tgccgcgctg
      181 cgcggtcttc ggcttggcgg cggtcgaggc tctttgaacc atgcgggttc ggactggaat
      241 gacagcgggg cgtttcatcg gcgctaatct gccgtgtcgg cgttttgagt aaagactccg
      301 ttgcgggaat cgcgaggccg tcctcgctga gggatggaac ctgccccagg ctcgatcgca
      361 ccagatgagc cccctggccg cacgcacttc acgctcgtca aacccgaccc attgggctac
      421 ttttccgccg tttatgtgca gaacgtgggt cttcctgcga ggcggtcttg tcgggtctct
      481 ctgtctggcg tcactcgcct ttccagccga tccggcgggg cggcccaccc caacaccacc
      541 gccattgccg ccggcaccgc cggcccgcgg gacgcccgcc ggcccggttt cggcccgagc
      601 gccggcgtcc gcggcgtctg tgtcgacgaa cagcgcggcg gcgtctagcg cgacacccga
      661 gcgcgccggg acggtgaatc agcgggtctc gcccggggcc gtcagcgtcc cgttgtccga
      721 tccgtcctac atcccgggct tgaccccggc gatcgtggtt gggccgcccg tacctctggc
      781 gccgcgagag cccgggatgc ggttcccgta ctcgacgcca cggcccgcgc gcgtccagga
      841 gaccgtgttc gcctatgacg ctttgatcaa ggaaaccacg ctcaaggcgg gcgagacatc
      901 ggcctatttc acctttcacc tgacgaatac ttcacccgag gcggtcacca tcaacgccgt
      961 gcgcacatcc tgcggttgcg ccgtcgcgaa gttgccgtcc gttccgtggc gaatcgaacc
     1021 gcggtcgggc ggcgcgttcg atgtggtagt ggacgttcgc ggctcgaggg gcgtgaccac
     1081 gaagactgtg acgatcgaca gcacggccgg ctaccgctac ctcacggtgc gtgtggcgat
     1141 gcccgagtcg gcgtcgccga tggccgcggc ggaccgcgcg cggaacctgc aggttgcgtt
     1201 ggcggatcgc caggcggttt tcaaggggga ttgtgccgcg tgccatttgc agcctgtgat
     1261 tgcgcagcat gggcaggcgc tgttcaagag cgcctgcggc atctgccacg aggcccaacg
     1321 ccgggctgcg gcggtccccg acttgcgggc cctgaacaag cccacggatc gggcctactg
     1381 gtcgggctgg attgggaagg ggaaagcggg cggcctcatg ccggcctggg ccatcgatga
     1441 gggagggccg ttgaccgacc gccaaatcga gtcgttgctc gattacctca caggcccttt
     1501 ccgagacgaa ccggctcgcg cgccgctggc gccgggggcc ccgacacgat agggcacaac
     1561 gccagccgtc cgatagaggg cgttggcatt catggctgga tcaggattat gcggcctcgt
     1621 ttcttttggt ccaagacgcc gcactggtgg aggccgagca aatccgtgcg agcggtctgg
     1681 cacaccacat tgtggctctt ctggtggctg gagatgttgt attcctgaaa cggatgtttg
     1741 accgaagtca gcattattgg cgggtcaaac catagtcatg cctcgatctg ggttcaccgc
     1801 ccgggcttcc ggcgaatggc acgaccacga cagatcgccc cggtccagga tgataagctc
     1861 gctcgtagtt gccgcgcagt tcgctcaggc tcgcccgcgc cacgtcggtg agtttcatct
     1921 cggtgtcctt cgaggtggac gaacgctctg aatcctcgat gatgttctgc cgaccgctct
     1981 gcgcggcctg ggtcatctgg tcgaattgcc ggtctttggg atcgtagttt tttccgctgg
     2041 cttcccgatg gtcatgcgtc aggaaccggc ggcagaagcg cagcgtctcg agctgcacaa
     2101 tgcggatctg acctattgcc tcaccgcacc agggagagaa tcgccttgaa atcgtcggtg
     2161 cccgccttgt cgagcaactc gaacagggcc atctcattgg acgcgatcac ggcgccggcc
     2221 tggcgcatgc gatccagacc caggcgccgg ttctcgggtg tgcgcgacga aacggcatcg
     2281 gcaatcacga acgggcggaa accgtgttcg agcagatcga gcgccgtctg gcagacgcag
     2341 acatgcgcct cgatcccgca gacgatcacg tccttgattg cccgggcgcg catcgcatcg
     2401 atcaggccgg gcgcgccgca ggcgctgaag gcgagttttt cgaacggagc aaacccgggg
     2461 atcgcctcgg ccacctcggg caccgttggt ccgagtccct ttcggtactg ctcggtggcg
     2521 aacagcggca cgccgaggac gctcgcgcct cggatcaggc agagcgtttc ggagacgacc
     2581 cggtcgcgct cgaaaatagc cggcaacagc cgctcctgga tgtcgaccac cagcacgccg
     2641 gtggcagtcc gatccaaacg ccatgaggtc tgggacataa gcaatgttga gtcaacgagg
     2701 gcttgtctgc atctcagccg cctaagggtc tgtgcaaaaa tcaattccgg ttttgctcga
     2761 ggggatttgg ctcgccggcg aggcgcgacg agggagcata cccgcggcgg tctgtgaccg
     2821 aagagcaatc ccgcgtccgc gggaccatcg agccaaagcc cccacccctt cggggcgggg
     2881 cggcaccggg ccatctgcgt cgttcctcgg tcggtcgagt atctcagaga gccggctgcc
     2941 agccggcgct ccggggcgcc tcgggacctc gccacccgcg cgcgaagggc cgcttcgcga
     3001 cctagcagcc cacgctgacg gatgcggccc gttacttgac agagaacttg tagatcgtgg
     3061 cggtcttgta agtctttttg ggacggagga tggtggacgg gaagttggga tggttgaccg
     3121 aatccgggaa atgctgggtc tcgagacaga atccatagta ctggtggtag acggtcttgc
     3181 ctttgccttc gagcgtggcg tcgaggaaat tcccgctgta gaactggacg cccggctctt
     3241 cggtgaatgt ctcgagaacc cgtccggtgc caggctcgta cacggtggcg gcgagccgca
     3301 tgcctttgcc ggagtcattg agggcgtagt tgtgatcgta gccgcgcggg ctgttgtcga
     3361 gggacagcag gcgcgagcca atgggcatcg gcgtggtgaa atccatcacg gtgcctttga
     3421 cgggggcgat cgcgccggtc gggatcaggg tgtcatcgac gggcgtgtag tggtccgcgt
     3481 tgatggtgag gacgtggttg aggatgtcgc cgcccccggc cagattccaa taggtgtggt
     3541 tggttaggtt gaccggcgtg gcctggtccg ttttggctgt gtaatccaga cgcagttcat
     3601 ctgtgtcggt caatgtgtac gtgaccgtcg cggtcagggt tccgggatac ccctcttcac
     3661 cgttcggact gacgtaagtg aatcggacgg ctgcgccgtt cctggccggg acgatctcgg
     3721 ccttccaaac gaccttgtcg aaaccgcgaa tgccgccatg caaatggttg gggccgtcgt
     3781 ttcgggccag cgtgtaatcg cggccgtcga gggtgaactt gcccttggcg atgcggttgc
     3841 cgacgcgtcc gatggttgcg ccgaagtagg gatgtccctt gaggtaggat tcgaggttgt
     3901 cgaagcccag aacgatgtcg ccgagcttgc cctgccgatc cggcacgtgc agttcggtca
     3961 tgagcgcgcc gtaggtgatg atcttggcga caatgccgtt ggcgttggta agtctgtatt
     4021 gctcgatgac cgtgccgtcg ggcatcgtgc cgtagtggga tttcttgacg ccggacggca
     4081 gggtggccgc ggcggacggg gtcaacgtca ggagcatggt gatgcaggct gccagggtcg
     4141 ggatgtatct gttcatggtc ggattcgagt tgtcggcgtt gggtgggggc ggtcaaggat
     4201 tctggcgcga gcccgggtcg cgggcggtgg gctggtccgg acggtttttg gcacgccagg
     4261 cgaacagggc catgaggcct ccgaagagca ggaggagaag cccccaccag aggttgatgt
     4321 tgattcccag cgagcgctgg tagctttcgg ggctggagag cgccccggtg aggaccagga
     4381 tggctccgac cagcgtgaac atgagaccga tgggccagcg gatatcgagc atataaatca
     4441 attggagtgc agtttgcgcc ttaccagaag attatgttga gaaccacgca gccggccagg
     4501 agcgcccagc cgatggtcgt cggtttcaga taccaggcct gttcctcgtc cttgagcttc
     4561 ggggtcaggg agtagaccag gccctttagt tcctcgttcg tcttggtccg ctgcgtggcg
     4621 agcgagatga ccagggtcag caggaaacag gcgctgaacg cgaagctggc gagccagaaa
     4681 ttctgagcca tttcgctggg gaaggactgg gcgacgccca ggtaccctcc cttgaggccg
     4741 gggttgttgc cggcggccag ggtgagcgag tggaacaacg ccgaggtcag cgtaccgccc
     4801 aggagcccgt aaaaggcgcc gtgcgaagtg gtgcgcgccc agaacatccc cagcaggaag
     4861 gtggcgaaga ggggggcatt gacgaagccg aacacgagtt gaacgatgtc catcgcgttc
     4921 gagtaattgc tggcgaagta ggcgcaggca atgctcagga gcacgcccgc caccgtcacc
     4981 acgcgcccca tccacatgta gtgtccatcg ctcttgttgg ggcagatgta ggcctggtag
     5041 aggtcgtagg tccagaccgt gttgaaggcg gtcacgttgc ccgccatgcc cgacatgaac
     5101 gacgccagca gggcggtcaa ggcaaggccg aggagcccgg acgggcagta tctcttgacc
     5161 agcgagagaa tcacgccgtc gtaatcggtg tcggcgacgg cgttctgcag atgattgcgc
     5221 agcgacttgt cgtcgagtgt cggagcggct tcgagcaacg cggcgacccg atcggggaac
     5281 atcttcacgc cggcggcttc gccgatgcgt ttcgcggcgg cttcggcatc gagcttctgg
     5341 ctggccgctt ctttcacggc cggaacggct ttggcgtagg cgctctcgac cagcattttg
     5401 ggcggaagcc gatagccgtc tttgcccagc gacgccaggg tcacggcgat catgcccggc
     5461 acgatcacca gggcggggaa gagcatcttg gggagggcgg ctatcagggg tgtgcggcgg
     5521 gcggcggaca tgttccgggc cgccatggcg cgctggacca ccaggaagtt cgtgcaccag
     5581 tatccgaacg acagcacgaa cccgagcccg aagaccatgg cgaacagatc gacgcccatc
     5641 ggattggctg tcgggccgcc gaggagcggt ttccaggcgc ttgaccatgc gtcgggggca
     5701 tagctaccgg gggccagtcc gagtcgggcg gggtcctggg ccacattcga gagcgagtgg
     5761 gtcatcgtct cccatccgcc aacgtctttg aggcccaggt agacgaccgg ggcgaacccg
     5821 aggacgatca tgaagaactg gagcacctcg gtgtagatgg ccgaggttag gccgcctttg
     5881 aacacgtaga tgagcacgac gcccgagcaa atccagaggc tcaggttgta gtcccagccg
     5941 agcaactggt gcagcagctt ggccagcgcg ttcatcgaga ttcccgatgc gaacagcgtc
     6001 atcacggcga acgtgatcga gttcaggcac cgcgttcgct catcgaaccg catcttcaga
     6061 tactcgggga cggaccgggc cttcgagccg tagtagaacg gcatcatgaa gatcgccagg
     6121 aacaccatcg cggggatcgc gcccacccaa tagaaatggc tggtggcgat gccgtacttg
     6181 gccccgctgg cagccattcc gaccagttcg agcgccccca ggttggcgga caggaaggcc
     6241 aggccggtga tccaggctgg gatcgagcgg ccggagagga aaaaatcctc gctgcttttc
     6301 atgaaccgtt tgagggcaaa tccgatgccc agcacgaaaa gcgaatagat gatgagaatg
     6361 gaatagtcga ttccgcccag ttgaatgctt tgcatacggt ccagtgccaa tggtgtaccg
     6421 gcccattccc cccttgtcaa tcaagcgagc accgagattt agcttgagct ttgggcgcga
     6481 cttgtgtggt tatatggccc tttattgccg tgccatgtca attcctgcga aacacttgct
     6541 gagtctggag aagatggacc cggaggccct ccggcacatc ctgaccgcaa cgaccgaact
     6601 caagcgcgac cggggccgcg tctgcgcacg tccgctgacc gggcagagct gggccttgat
     6661 tttcagcaag tcctcgaccc gcacgcgcgt ctcgttcgag gtgggcatcc gcgagttggg
     6721 cggccaggcc ttattccttt ccgcgagtga aatccaggtc gggcgtggcg agtcgatcaa
     6781 ggacacggcg cgcgtgctgg ggcgcatgat ccagggcgcc gtgatccgca cgtatgccca
     6841 gcaggatgtc gaggagttcg cccactactc cggcattccc acgatcaatg cgctgaccga
     6901 tgccgagcat ccgtgccaga tcctggcaga cgtgttcacg ttcgaggaga aacgcgggcc
     6961 gattgccggg cgggtggtgg cgtttctcgg ggatggcgcc tgcaacgtgg cgtcgagctg
     7021 gatatgggcg tctgccaggc tcggttttga attgcgcatc gccgctccca aaccctacca
     7081 gcccccggcc gatctgctcc gccgcgcgca tgcccacgtc cagtgcaccg acgatcccgt
     7141 cgaggccgtt gccggagccg acctgctcta caccgacgtt tgggtgtcga tgggcaagga
     7201 ggccgaggcg tcggatcgcc tgagcctgct caaggatttt cagctcaacg cgcgcctggt
     7261 caaagaggcc aagccggacg ccctggtgat gcattgcctg cccgcctacc gcggcaagga
     7321 gatcgatgcg gccacattcg aggcgaatgc cgcgacgatc ttcgacgagg cggagaatcg
     7381 gttgcacacg cagaaggcgg tgctcagttg ggtggtgcgc ccggactgag tgaagtccgg
     7441 ctgcctgttc cgcgtcctgt tgctccccgg ccccggcgct ctgaacgttg tgaaagagag
     7501 aaccgctgca gtgcacctgt gagttgtcgg tgggaatgga gtttgcgcgg cgtgggggcc
     7561 tgcctgccgc gcctgcttgt gccgtgcccg acacggcaga caggccgctg cgctttacgg
     7621 cacagacagg gcacgcggcc tacagcggga gtctccatcc gttgtaggcc cggtgccctc
     7681 accgggctac agaataccga caactgtcag atgcaccgga accgccgggg ggacggcgca
     7741 cgcgcttgac gcaacccggt tcgctggtat tgtcgcgcct ctttgagatg gaacgtaccc
     7801 tgcgcagacc gcgatggcaa cgggatttgc cgtgcgggcc ggggtgcggc acagcgatcg
     7861 catttgcatg aaacgccaat accaaccatc gaagattcga cggaaacgcc agcacggttt
     7921 tctggcccgc aacgcaagca aacgaggccg ggcgatcctg gcgcgccgcc gccgggtcgg
     7981 acgcaagcgg ttgacccccg tctgagccgc gcgctccggc gaggggtggg ggaagccgtg
     8041 ccgggcatcg gcttgggcgc tggctcttcc tcgacgggcg aggtttcgcc ctcgccgcct
     8101 cgtctggcgc cggccaactg tcttcttcga gtgactgtcc agagaccacc ggccttgacc
     8161 ttgccgcgca aggcgcgcct tcgccgcccc ggtgagtttg ccgatgtccg ccaacgcggg
     8221 cgtcgcgttg tgcgcggatg tctcatcgcg aactgggtcg gcctgccggg cggggcgccc
     8281 tcgaagctgg gtgtggtgac cgggcgcaag gtgggcaagg cggtcgagag gtcgcgcgcc
     8341 cgccggctgc tgcgcgagac cttccgcctc catcagcacg atctgcgcgc cccggtcgcc
     8401 ctggtgctgg tggctcgccc ctcgatccgg ggccaaacgc ggatggccgt cgagcgtgat
     8461 ttcctggctg ccttgcgtgg cgccggactt cttcgcccgg cgccgagcgc ggtcggggca
     8521 cgaacgccat gaacccggcc caacatctcg cggtgggtgc gattcggttt taccgatggg
     8581 tggtgtcgcc cgccaagact gtcctgtttg ggccacttgg ccggtgccgc tacaatccat
     8641 cgtgttccgc gtatgccctg gaagccgtgc gcacgcacgg gacccttcgc gggctgtggc
     8701 tcgccctccg gcgattcggt cggtgccatc cctggggggg ctgcggtgac gatccggtgc
     8761 cgccggcgcg caccccgtcc gccgaaagcg ccgcgtcttc gcgcctcata ccctccgcat
     8821 cgaggcctgt caccgcgctg tccgaggcgc tcactccaca gggtcccgcc tgagtttccg
     8881 tcatggaccg caagtcgatt ctcattcttg ttctctgctt cgttctgctg atgctctggc
     8941 ccgcgttggt gaacaagatt ttccccccgc cgaagcagac cgggagcacc aacatcgtcg
     9001 cgggcgccgc atcgaccaac ctcttcggcc tgaccaacct cccgccctcg accgagggcc
     9061 cgggaggatc gggtgcggcc gcggccttgc ccgccatcac cgcccccagc tcgggcgcgt
     9121 ttgcctggac gccaccgacg ggccccgagg aaaccatcaa ggtcgagtcg accgatgcga
     9181 tctacacatt cacctcgcac gggggcggtt tgaagacggt cgagctgaag ggcacagtcg
     9241 agaccgtgcc atgcgggcgt gggaaacgcg ccgctagcga caagttggcg actttgaatc
     9301 gagacgcgcc gatccccgcg ctggctctct ttgccggggc tgggctcgag gatgggcgtc
     9361 catttgcgat gcgccggacc gagggggggg tgactgtcga gaaggctctg accaacggct
     9421 tgcgcgtggt gaaggagttc ctggttcagt ccaattacat ggtcgcagcc gccgtccgga
     9481 tcgagaaccc ggggagcgag ccgatcgccc ttcccgcgca ggagctggtg attggcgcgg
     9541 cgactccgat ggggccctat gacgacggca cctacgtgcg attggaatgg tacgacggcg
     9601 ggcgaacggt gtcggcggat cggagctggt tttccaaccg aggcctcttc agttgtctgg
     9661 gtctgggcgg cgcgccgcgc cccgagtacc tgagtccggg cgccagcaat gtggtttggg
     9721 catcgcttca gaaccagttc ttcacgatgg tggcgattcc tcaacagacg ccgcatggat
     9781 tggtggcgcg tccggtcgat ctgccgcccc cgagcatcga ggagcgggaa cgcgactcga
     9841 ggatc
//
LOCUS       DAOQRO010000099         9841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6224_length_9841_cov_5.078786, whole genome shotgun sequence.
ACCESSION   DAOQRO010000099 DAOQRO010000000
VERSION     DAOQRO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_9841_cov_5.078786"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PKK20_05385"
     CDS             <1..701
                     /locus_tag="PKK20_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNU99349.1"
                     /translation="SDRDNPGRPRGWRGRGGPDGYPFGRPFWMSEQQYKTAVQQKGVH
                     SFLLVLSTQSLQASVRQDFWLRAIISAMAAISVAGLGLAWQSLTKTSELEVRLIRASE
                     LNARLKEMNLAAAGLAHETKNPLNVIRGLAQMLSKQANASPEIRRQSDGIIAQTDRVT
                     AQLNEFINYSSPREVRRTAVNLNSVVSEVVRALGFDVEEKKVRLEVTHDMPSVEADEQ
                     LLRQALFNLLLNAT"
     gene            705..974
                     /locus_tag="PKK20_05390"
     CDS             705..974
                     /locus_tag="PKK20_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99350.1"
                     /translation="MGTGGEIRVSADKTSATEARLDIRDDGPGVPPEHREEIFKPYFT
                     TTERGTGLGLAVVHQIVLAHGWDVACLPNEPRGALFRLSHLRLAN"
     gene            1601..2752
                     /gene="hemW"
                     /locus_tag="PKK20_05395"
     CDS             1601..2752
                     /gene="hemW"
                     /locus_tag="PKK20_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNU99351.1"
                     /translation="MPFCARKCHYCAFYSETANASLIDQYVTALIRELELVAPDLRPR
                     TVYFGGGTPSLLNLRQWERILDAMGRLDLLGADEWTVETNPATVSLDKAKLLESHGVN
                     RISMGVQSLDQPLLERLGRIHTRDMVFRSFDTLRRAGFDNLNLDLMFAIPGQTMPVWR
                     ATLREAIALGSEHLSSYEVIYEEDTPLFQQLKASRLDVDDDLACDMFEELAAQAAAHG
                     YRQYEVANFAKHLGPQPLGEVPDRACRHNVNYWRGGSFYGLGPSATSYVRGVRTKNWS
                     STHLYCQLLAKGQRAFESTETLSPMSRAGETAAFGLRMNAGWPFDEFLRVTGFDLRVE
                     WRNDMAELERQGLAKLRPDRFQLTDRGLRFADLAAERFLRPFMGSRSRF"
     gene            complement(2905..3732)
                     /locus_tag="PKK20_05400"
     CDS             complement(2905..3732)
                     /locus_tag="PKK20_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99352.1"
                     /translation="MITKPNQSRGAIRPGFTLIELLVVIAIIAILAGMLLPALSKAKA
                     KATGIVCMSNTKQLALAVLMYTHDNADWFPPNRDGGTKDVNVSWVAGWLDWNTGNTDN
                     TNTYFLLKAKIGAYTSDVGIYRCPADNYPVNMGRTKALRVRSNAMNGFIEGGAYSLNT
                     ASGSTWYPNYCMYNKVSDVIRPAPAQLWMLNDEHPDSINDGWEITNVTDPNNWTDLPA
                     SYHNNACGFNFADGHSEIKRWLEKSTEVKIRRTQYNGFPAPKSRDIRWVIERSTALR"
     gene            complement(3737..4420)
                     /locus_tag="PKK20_05405"
     CDS             complement(3737..4420)
                     /locus_tag="PKK20_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNU99353.1"
                     /translation="MNEREHILARIREALTISAPVPGSHDGRPRRNGLPTSADQRRVL
                     PAVGDSFDARASLFARNAADLHAEFKLLANPCDLAAELKRLAAAEQWDRVASHRGALT
                     DPACAALGLPVLLTDEPYDKTELERCPAGLCACDALVAQTGSVLVTSRSAGGRALSVL
                     PPHHVVLARRDQLVADLPEAFALLRSKYGADYPSMISLITGPSRTGDIERIIVLGAHG
                     PRQLTILCF"
     gene            complement(4449..5846)
                     /locus_tag="PKK20_05410"
     CDS             complement(4449..5846)
                     /locus_tag="PKK20_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNU99354.1"
                     /translation="MSASASQFKRDAARLTADLRHRALIQTALGNYEIKRDEKKALFQ
                     DYDGARQLAAEIKWEAINRLDRHLEQFVRELETRGTKVHWATTARQACDIIVGIARSR
                     RARSIVKSKAMTAEEIHLNAALEQAGFEVVESDLGEFIVQLRDEPPYHIVFPAMHLTR
                     GEIQRTFKDKLHDAPSDNPEALTMVARRVLRQKYVQADIGLTGANFAVAETGMISVTE
                     NEGNARLTAALPKTMITLLGIEKIVPRLDDLALLLPMLATVGAGQLISGYNTLYGGPR
                     QPGESDGPEEFHVVLLDNNRTALLANAEQRDALHCIRCGSCLNVCPIYRHVGGHSYAT
                     TYPGPIGGVITPNLRGLQDWKHLSYSSSLCGACTEACPVKIDLAHHLLQNRRNASRRK
                     PIWWQKIAFKLFAPVANGRIPWMLVTKGGRFGQRFHGLIKGTRIDPAYCWTRTRDVPR
                     IAKQSFKEWWRETRK"
     gene            complement(5843..6556)
                     /locus_tag="PKK20_05415"
     CDS             complement(5843..6556)
                     /locus_tag="PKK20_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNU99355.1"
                     /translation="MTVTLYIPCFIDLIYPQVGIAMVRILEKLGHRVECPESPACCGQ
                     PAFNTGYWDESRATAVKVLDQLKDAEVVVIASGSCGAMIKVFYPDLFAGTPQEGLARQ
                     VSAKCFEFSQFLVDMLGVTDLGARFPHKVTYHDGCHSLREMHIHAAPRQLMARVQGLE
                     LVEMKQTVCCGFGGTFSAKFPMISTAMGEVKCELALETGAEYVVSSDSSCLMHIQGLI
                     DRRRQPLKTIHLAEILAPA"
     gene            complement(6614..8575)
                     /locus_tag="PKK20_05420"
     CDS             complement(6614..8575)
                     /locus_tag="PKK20_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99356.1"
                     /translation="MKTTLLNSLFAFTLPLALCTARADIPPAERLLPSDTLAVLCAPD
                     WSKVTAARQQAPFALLWKDPAMRPFCDKFLAKLTSDVVAPIERDLGAKLTDYADLLQG
                     QFTLAVTRNGWTGAGEPLPAFVLTLDARDKADLLRKNLADVKRKLTDSGRKTRTDTIR
                     GVEFTTVILDRPQLEGGLGTIGGAGAGAGSANTQKSGAASAASSPSGQAGSTIELSVG
                     QADSALLVGTSTKVLEQVLARMAGAGGGALADLPAFEADASGVLRDSLLYGWIHFAPI
                     AETLARLFPAGEGTPDGEPAAMMAFGLDKMLGALGLSCLRTLAFNARQNAEGEFFDFY
                     LGVPEETRKGLFAVLNATPKDASPPAFVPAEAVNFSRWRADGQRIWASVEAIANEIAP
                     GMLGLVVMQVEAMIKERSPAFDLRKNLIANLGDDFISYQKAPRGTALDDLISPPSVTL
                     ISSPNPDQLLQGLKSVLSMLAAPLSSAGFKEREFLGRKIHSLPMPTLPTKEGSAAAEE
                     SVHMTASGGYLAVSTDAALVEEYLRSGETKPKPLAALPGLSEAGGKVGGMATGLFGYQ
                     DDVENLRTLTEILRNNGDLISQALATSPIGPKSGEPDKALKEWFDFSLLPPFERIAKY
                     FHLTVYAGQTSKEGFTLKTFSPRPPKLNP"
     gene            complement(8628..9677)
                     /locus_tag="PKK20_05425"
     CDS             complement(8628..9677)
                     /locus_tag="PKK20_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNU99357.1"
                     /translation="MLRRSMYCAGAAFGLAWALMADVIQLKDQAAITGTILAEKPDQL
                     VVDVGYTVLIVPRERVETITRTGAGNAPSEPGRESAAGPKTAGTGGSAPDAGNATVAP
                     APGLSGAAGRGGLFAASSGAGQDRSVRELVKELGEAVVQVRAPSGLGSGFFINDEGYL
                     ITNFHVIEGETQISVEVYHQRNGELERKTYKQVKIVAMNKFQDLALLKIEDAGAPRFV
                     SVVLGSTRTVAVGERVFAIGSPHGLERTVTEGILSTKTRQLQGELLLQTSAQINPGNS
                     GGPLFNLRGEVIGVTNMKINFGEGLGFAIPVERVRHFLENRDAFAYDNDNPSNAYRYL
                     EPPRRLQKAIGQPAL"
BASE COUNT         1647 a         3135 c         3225 g         1834 t
ORIGIN      
        1 aaagtgaccg cgacaatccc ggacgcccgc gggggtggcg cggacgcggc gggccagacg
       61 gctacccgtt cgggcgcccg ttctggatga gcgagcaaca atacaagacc gccgtccagc
      121 agaagggcgt ccatagcttc ctgctcgttc tctcgactca atcgctgcag gcgtccgtcc
      181 ggcaggactt ctggctgcgc gccatcatca gcgcgatggc ggcgatctcg gtggcgggcc
      241 tgggtctggc gtggcagagc ctgaccaaga cctcggaact cgaagtgcgc ctgatccgcg
      301 cctcggaact caacgcccgc ctcaaagaga tgaacctggc cgccgcgggc ctcgcccacg
      361 aaaccaagaa cccgctcaac gtcatccgcg gcctcgccca gatgctctcc aaacaggcca
      421 acgcttcccc ggaaatccgg cgacaatccg acggcattat cgcccaaacc gatcgcgtca
      481 cggcccaact aaacgagttc atcaattact cgagcccgcg cgaggtgcgc cgcacggccg
      541 tcaacctcaa ctcggtcgtc agcgaggtcg tgcgcgccct cgggttcgat gtcgaggaga
      601 agaaggtgcg gctcgaagtc acccacgaca tgccctcggt cgaggcggac gaacaactcc
      661 tgcgccaagc cttgttcaac ctcctcctca atgccaccta ggcagtgggc acaggcggcg
      721 aaatccgcgt cagtgccgac aagaccagcg ccaccgaggc gcgcctggac atccgcgacg
      781 acggccccgg cgtgccgccc gaacaccggg aggaaatctt caaaccttac ttcaccacca
      841 ccgagcgcgg caccggcctg ggcctcgccg tggtccacca gatcgtcctg gctcacggct
      901 gggacgtcgc ctgcctccca aacgaaccgc gcggcgccct ctttcgcctc agccacctcc
      961 ggctcgccaa ctgagccgcg cgacagagcc gcccgttccc ttttagactg cccagtggac
     1021 atgaaccgat ctgatagggg acgtcctcgt ccgcggcgat gagagttgca gaccctgttt
     1081 ggcgggcgac gaggacctgt ctgccctgcc tgccctgcct gccctgcctg ccggcaggca
     1141 ggcggcaggc aggcggcagg caggcggcgc ccctccggca atcgccatcc gttgcaggcc
     1201 cggtgccctc accgagcggc attacaccga aacgaacgga tgcaccgcgt gatcgtgctc
     1261 gactcgctgc tttatccgaa tgatcctccg cgccgttggg gggcgatcgc actcaagacg
     1321 gcaagacatt tgacctccga tggagttgaa actcgccgct cagggtcccg acatggatcg
     1381 cctggtcatt caacgatcca agccgcatga gggcccagaa cctctggagc ggggactgcc
     1441 tgtccccggt tacggcaggg gacgagacgt ctcgcctccc ggccggttca tgggcgagtt
     1501 cgtgtgaggg cggagaggaa ggatgatggt cccagcacgt tcccttcgcc caaacgcgcc
     1561 gaagccgccg agtgcaccga tcggtcacct ctatatccat gtgccgtttt gcgcccgcaa
     1621 atgccactat tgcgctttct attcggagac cgccaacgcc agcctgatcg atcaatacgt
     1681 cacggccctg atccgcgagc tggaactggt cgcccccgac ctgcgcccgc gcaccgttta
     1741 cttcggcgga ggcaccccct cgctcctgaa cctccgccag tgggaacgaa tcctcgacgc
     1801 catgggccgg ctcgatctgc tcggcgccga cgagtggacg gtcgagacca atcccgccac
     1861 cgtgtcgctc gacaaggcca aactcctcga atcccacggc gtcaaccgca tttcgatggg
     1921 cgtccaatcg ctcgaccaac ccctccttga acgcctcggc cgcatccata cccgcgacat
     1981 ggtgttccgg tcctttgaca cactccggcg cgcgggcttc gacaatctca acctcgatct
     2041 catgttcgcc atcccgggcc agacaatgcc ggtgtggcgc gccacactcc gcgaggcgat
     2101 cgccctcggc agcgaacacc tctcctccta cgaagtcatc tacgaggaag acacccccct
     2161 gttccaacag ctcaaggcca gtcgcctcga cgtcgacgat gatctcgcct gcgacatgtt
     2221 cgaggaactg gccgcccagg ccgctgcaca cggctatcgc cagtacgaag tggccaactt
     2281 cgcaaagcat ctcggccccc agcccctggg cgaagtcccg gatcgcgcct gccggcacaa
     2341 tgtcaactac tggcgcggcg ggtcgttcta cggactcggc cccagcgcga ccagctatgt
     2401 ccgcggcgtg cgcaccaaaa actggtccag cacgcacctc tactgtcagc tcctcgccaa
     2461 gggccagcgc gccttcgagt ccaccgaaac gctctccccg atgagccggg ccggcgagac
     2521 ggccgcgttc ggactgcgca tgaacgccgg ctggccattt gacgagttcc tccgcgtcac
     2581 cgggttcgac ctgcgagtcg agtggcgaaa cgacatggcc gaactcgaac gccagggcct
     2641 ggcgaagctg cgccccgacc gcttccagct tacagaccgc ggcttgagat tcgccgatct
     2701 ggcagccgaa cgcttcctgc ggccattcat ggggtcacgg agccgcttct gaggggggcg
     2761 tgcggttgga tcggcttgga ttcctgatga atgcgcggtt ttcattccct gccggacagg
     2821 gaactgctcc ccccgccccg caggcaaggc tcctgatccg ccaggcgcac gactggcggg
     2881 gttcggcgtc gtgcccgtga tcggtcatct caacgccgtg gagcgctcga tgacccacct
     2941 gatgtcccga ctcttcgggg cggggaagcc gttgtattgg gtgcgccgga ttttcacttc
     3001 ggtgcttttc tcgagccagc gtttgatctc gctatgacca tcggcgaagt taaacccgca
     3061 ggcgttgttg tggtaactgg ccggcagatc ggtccaattg ttggggtcgg tgacgttggt
     3121 gatttcccat ccgtcattga tgctgtcggg gtgctcgtcg ttgagcatcc acaactgcgc
     3181 cggcgccggt ctgatcacat cggacacctt gttgtacatg cagtaattcg ggtaccatgt
     3241 cgagccgctg gccgtattga ggctgtaagc gccgccctcg atgaaaccgt tcatggcatt
     3301 gctgcggacc ctgagcgctt tggtgcggcc catattgacg gggtaattgt cagccggaca
     3361 gcggtagatg ccgacatcgc ttgtgtaggc gccgatcttg gcctttagga ggaagtaggt
     3421 gttggtgttg tccgtgttgc ccgtattcca atccagccat ccggccaccc agctcacgtt
     3481 cacatccttc gtgccgccgt cccggttggg tggaaaccaa tcggcgttgt cgtgggtgta
     3541 catgagcacc gcgagcgcca gttgtttggt gttgctcatg cagacgatgc ccgtggcctt
     3601 ggccttggcc ttgctgaggg cgggcaggag catgccggcg aggatcgcga tgatcgcgat
     3661 cacgacgagc agttcgatga gggtgaagcc ggggcgaatg gcgcccctgc tttggtttgg
     3721 tttggtgatc atagcattaa aaacagagga tcgtcagttg cctggggccg tgggccccaa
     3781 ggacgataat gcgttcgatg tcgccggtcc ggctggggcc cgtgatcaat gagatcatgc
     3841 tcggataatc ggcgccgtat ttcgacctga gcagggcgaa cgcctccggc aaatcggcca
     3901 ccaactggtc gcgtcgagca agaaccacat gatggggcgg caggacagac agagcgcgtc
     3961 cgccggcgct gcggctggtg accaggacgc tgcccgtctg ggccacgagt gcatcgcatg
     4021 cgcagagccc ggcgggacat cgttcgagct cggtcttgtc gtaaggctcg tccgtgagga
     4081 gcacgggcag gccaagggcc gcgcacgccg ggtcggtcaa tgcgccccgg tggctggcca
     4141 cgcgatccca ctgttcggcc gcggccaggc gtttcagctc ggctgccagg tcgcaggggt
     4201 tcgcgagcag cttgaattcg gcgtggagat cggcggcgtt tctggcgaac agggacgcgc
     4261 gggcgtcaaa ggaatccccg acggcgggca ggacacggcg ctggtcggcg ctggtgggca
     4321 ggccgttccg gcggggccga ccatcatgag aacccggaac gggagcagag attgtcaggg
     4381 cctccctgat gcgggcgagg atgtgttcgc gttcgttcat ggcattgtgg gcggacggct
     4441 ggcggcggtc atttgcgcgt ctctctccac cattccttga agctctgttt tgctatgcgc
     4501 ggcacgtcgc gcgtccgggt ccagcaatac gccggatcga tccgtgtgcc cttgatcagg
     4561 ccatgaaacc gctgtccgaa gcgtcccccc ttggtcacca gcatccaggg aatccggcca
     4621 ttggcgacgg gggcgaagag cttgaaggcg atcttctgcc accagatcgg tttgcggcgc
     4681 gaggcattgc ggcggttttg gaggaggtgg tgagccaagt cgatcttgac cgggcaggct
     4741 tcggtgcaag ctccgcagag tgaggaggag taggacagat gtttccagtc ctggaggccg
     4801 cgcaggttgg gcgtgatcac gccgccgatc gggccggggt aagtggtggc gtaggagtgg
     4861 ccgccgacgt ggcggtagat tgggcagacg ttgaggcaag aaccgcagcg gatgcagtgg
     4921 agcgcgtctc tctgctcggc attggcgagg agggcggtgc ggttgttgtc gagcaacacc
     4981 acatggaact cctcggggcc gtcagattcg ccgggctggc gcgggccgcc gtagagggtg
     5041 ttgtagccgc tgatgagctg gccggcgccc accgtcgcga gcatcggcag caagagggcc
     5101 agatcgtcca gccgcggcac gatcttctcg atgccgagca gcgtgatcat ggtcttgggc
     5161 agcgcggcgg tgaggcgcgc gttcccctcg ttttccgtga ccgagatcat gcccgtctcc
     5221 gcaacggcga aattggcgcc ggtgaggccg atatcggcct gaacgtactt ctgccgcagg
     5281 acgcggcggg cgaccatggt gagagcttcc gggttgtcgg aaggcgcgtc gtggagcttg
     5341 tccttgaagg tgcgctggat ttcgccgcgg gtgaggtgca tggccgggaa gacgatgtgg
     5401 tagggcggtt cgtcgcggag ctggacgata aactcgccca gatcggactc gaccacttcg
     5461 aacccggcct gttcgagggc ggcgttgagg tggatctcct ccgcggtcat ggccttcgac
     5521 ttgacgatcg agcgcgcgcg cctgcttcgg gcgataccga cgatgatgtc gcacgcctga
     5581 cgcgcggttg tggcccagtg caccttcgtt ccccgcgttt cgagttcgcg cacgaactgt
     5641 tcgaggtgcc ggtcgaggcg attgatggcc tcccacttga tttcggcggc gagctggcgc
     5701 gccccgtcgt agtcctggaa gagcgccttc ttctcgtcgc gtttgatttc gtaattgccg
     5761 agggcggtct ggatgagcgc ccggtggcgc aggtcggcgg tcagccgggc ggcgtcccgt
     5821 ttgaattggg aggcggaggc gctcatgcgg gcgcgaggat ctcggcgagg tggatagtct
     5881 tgagcggctg gcggcggcga tcgatcagcc cctggatgtg catcaggcag ctcgaatcgc
     5941 tcgacacgac atactcggcc ccggtctcga gcgcgagttc gcacttcacc tcgcccatgg
     6001 ccgtcgagat catcggaaac ttggcggaga aggtgccgcc gaagccgcag cagacggtct
     6061 gcttcatctc gaccagttcg agtccctgca ctcgggccat cagttggcgc ggcgcggcgt
     6121 ggatgtgcat ttcgcggagg ctgtggcagc cgtcatggta agtgaccttg tgggggaacc
     6181 gggctccgag atcggtcacg cccagcatgt ccacgagaaa ctgggagaac tcgaagcact
     6241 tggccgagac ctgcctggcc agtccctcct gcggcgtccc ggcgaacagg tccgggtaga
     6301 acaccttgat catcgcgccg cacgagccgc tggcgatcac cacgacctcg gcgtccttca
     6361 gttgatcgag caccttcacg gccgtggcgc ggctttcgtc ccagtagccg gtattgaacg
     6421 cgggctggcc gcagcacgcg ggactctcgg gacactcgac gcggtgcccg agtttctcga
     6481 gtatgcgcac catcgcgatt ccgacctgcg ggtagatcag atcgatgaag cacgggatgt
     6541 acagcgtgac ggtcatgggc cgacggtcgg ttgtttcgat cgcttcattc gaatcggtcc
     6601 ggggtgcgtc tcgctaggga ttcagtttgg gcggacgagg gctgaaggtc ttgagcgtga
     6661 acccttcctt cgaggtctgg ccggcgtaga ccgtcaggtg aaagtacttg gcgattctct
     6721 caaagggcgg gagcagcgag aaatcgaacc actccttcaa cgccttgtcc ggctcgcccg
     6781 actttggtcc gatcgggctc gtggccaggg cctgcgatat caggtcgccg ttgttgcgca
     6841 ggatttcggt gagggtccgg aggttttcga cgtcgtcttg atagccgaag aggcctgtgg
     6901 ccatgccgcc cactttcccg cccgcttcgc tcaggcctgg cagtgcggcg agcggtttgg
     6961 gttttgtctc tccgctgcgc aggtattcct cgaccaacgc cgcgtctgtg gagacggcga
     7021 ggtagcctcc gctggcggtc atatggacgc tctcttcagc cgccgcgctc ccctccttgg
     7081 tcggcagggt gggcattggg agggaatgga ttttgcgacc gaggaactcg cgctccttga
     7141 agccggcact gctcagcggc gctgcgagca tggacaggac cgacttgagg ccttggagga
     7201 gctgatcagg attgggcgag gagatcaagg tcaccgaggg cgggctgatg aggtcgtcga
     7261 gcgcagttcc tcgcggagcc ttctggtagg agatgaaatc gtcaccgagg ttcgcgatca
     7321 ggttttttct gaggtcaaag gcgggcgacc gctccttgat catcgcctcg acctgcatga
     7381 caaccaggcc gagcatgcct ggcgcaatct cgttggcgat ggcttccacc gacgcccaaa
     7441 tgcgctggcc gtccgcgcgc cagcggctga aattgacggc ttcagccggg acgaaagctg
     7501 gcggcgaagc gtccttgggc gtggcattca ggacggcgaa gaggcctttg cgggtctctt
     7561 cggggacgcc gagatagaaa tcgaagaact cgccttcggc gttttggcgc gcattgaagg
     7621 ccagtgttct gaggcagctc agcccgagtg ccccgagcat cttgtccagg ccgaatgcca
     7681 tcatcgcggc cggttccccg tcgggtgttc cctcgcccgc cgggaacagg cgcgccagag
     7741 tttcggcgat gggtgcgaag tgaatccagc cgtacaacaa gctgtcacgc aaaaccccgc
     7801 tcgcgtccgc ctcgaacgcc ggcaggtctg ccagggcgcc tccgcctgcg cccgccatgc
     7861 gcgccaggac ctgttcaagc accttggtgc tggtgcccac aagaagcgcc gaatccgcct
     7921 gtcccacgct caactcgatc gtcgatccgg cttgtcccga gggcgacgat gcggcagacg
     7981 ccgctccgct tttctgggtg tttgcgctcc cggccccggc gcccgcgccg ccgatcgtgc
     8041 cgagaccccc ttcgagttgc ggccggtcga gaatgacggt cgtgaactcg acgccgcgaa
     8101 tggtgtcggt gcgcgtcttc cggcccgagt ctgtcagctt gcgcttgacg tcggcgaggt
     8161 tctttctgag gagatcggcc ttgtcccggg cgtccagcgt cagaacgaat gccggcagag
     8221 gttcgcccgc acccgtccag ccgttgcggg tgactgcgag ggtgaattgc ccctggagca
     8281 ggtcggcgta gtccgtcagc ttggcgccga gatcgcgctc gattggggcg acgacatcgc
     8341 tggtgagctt cgcgaggaac ttgtcgcaga aggggcgcat cgccgggtcc ttccacagga
     8401 gcgcgaaggg ggcctgctgc cgggcggccg tcactttcga ccaatccggc gcgcagagca
     8461 cggcgagggt gtcgcttggc aggaggcgtt cggcgggcgg gatatccgcc cgcgccgtgc
     8521 agagggcgag aggcagggtg aaggcgaaga gcgaattcag aagcgttgtt ttcatgtgtt
     8581 cctgactggg tctggggctc tggagccggt ggccgtccga ggtggtatca aagcgcgggc
     8641 tgcccgatcg ccttttgcag ccgccgcggc ggttcgaggt agcgataggc gttgctgggg
     8701 ttgtcgttgt cgtacgcgaa cgcgtcgcgg ttttcgagga aatggcgcac gcgctcgacc
     8761 gggatggcaa aaccgagtcc ttcgccgaag ttgatcttca tgttggtgac gccgatgact
     8821 tcgccgcgca ggttgaagag cgggccgccg ctgttgcccg ggttgatctg agcgctggtt
     8881 tgaagcagga gttccccctg caactgccgg gtcttggtgc tcaagatccc ctcggtgacg
     8941 gtacgttcca gtccgtgcgg gctgccaatc gcaaagacac gctccccaac cgcgacggtc
     9001 cgcgtgcttc cgagcacgac cgacacaaac ctgggcgccc ccgcatcctc gattttcaac
     9061 aacgccaggt cctgaaactt gttcatggca acgatcttga cctgcttgta cgtcttacgt
     9121 tcgagttcgc cgtttcgctg atgatacacc tcgaccgaga tctgcgtctc gccctcgatc
     9181 acgtggaaat tcgtaatgag atacccttcg tcattgatga aaaaacccga tcccaatccg
     9241 ctcggcgcgc gcacctgcac caccgcctcg cccagttcct tcaccaactc gcgcaccgac
     9301 ctgtcctgtc cggccccgct cgatgctgca aacagaccgc cccgtccggc cgcgccgctc
     9361 aatcccggcg ccggcgcgac cgtggcgttc cccgcgtccg gggcggagcc gcctgtgccc
     9421 gccgtcttcg ggccggccgc ggattcacga cccggctcgg aaggggcatt tcccgcgccg
     9481 gtccgagtga tggtctccac tcgttcgcgc ggcacgatca acacagtgta gccgacgtcg
     9541 accacaagct gatcgggctt ctccgcaaga atcgttccgg tgattgccgc ctggtccttg
     9601 agctggatca catcggccat caacgcccag gccagcccga acgccgcacc ggcgcagtac
     9661 attgagcgtc tcagcatgga gaagacgata caagccggag cgcccgtcag caagcactac
     9721 cgcagagcgc gccggggagg tcgggaagcg gcggtgcatc tgtaggttgt cagtggggat
     9781 ggagtctgcg ccgcgtgggg cctgcctgcc gcgcctgcct gtgccgtgcc cgacacggca
     9841 g
//
LOCUS       DAOQRO010000100         9752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6316_length_9752_cov_5.290399, whole genome shotgun sequence.
ACCESSION   DAOQRO010000100 DAOQRO010000000
VERSION     DAOQRO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_9752_cov_5.290399"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4213
                     /locus_tag="PKK20_05430"
     CDS             <1..4213
                     /locus_tag="PKK20_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNU99358.1"
                     /translation="PVKKSPAPRRLSGWRLRGVGFTFPSGVVLGPGAFAVVASNRDGF
                     AAAYGFGVLPVGEFPGNLQNGGERLRLVRPGATPEEDELVDEVRYANEPPWPVQADGW
                     GPSLQLIDPRQDNWRVGNWAAAHPGDAVVATPGKPNSVAKTLPPFPPLFINEVQPQNV
                     SGPVDRFGDRDPWIELHNPGTNAIDLGGFFLTSTYTNRTEWAFPDGARIGPGEFLLVW
                     ADGEPVESTEDELHTRFRLAPRSGAVALVREQLGAPAVIDFINYQSIPDDLSTGSYPD
                     GQPQSRQLFHLPTPGGANDPGTPPVDVFINEWMASNTGVALDPADGDADDWFELYNAG
                     ARAVDLSAYTLTDNLEDPAKFVIPNGTVIPAGGFLLVWADDETEQSGQGQLHVNFRLS
                     AAGESLGLFTPDGRAVDSIAFGSQTNNISEGRFPDGMIRPPFVFMGRPTPGGPNDFPT
                     LNVPPTLDPVGSRTVDEGQTVAFTVSATDPDAGQTLVFALFGAPPAAQIDSRTGAFSW
                     TTTEADGPGAYAFTVRVTDDGTPPRWDAEEITITVREVNQSPTLDPIADQSVDEGSLL
                     SIQAVARDVDQPAQSLRFSLVGVVPEGATIDALTGEFSWIPTEDQGAQTYEITVQVAD
                     DSVPPGLATRGFLVVVNEVDNAPVFEPVSLQTVDESSPFALRLSARDPDSPPRAVIYR
                     LESGPPGLTLDPATGQVNWTPVEAQGPGSYNVVVRAIEIGGTQTSTLSFAIVVNEVNE
                     PPTLAAIADRTVAEGTVLSFINNAADTDLPRQTLRFSLDPGAPPGASIDPQSGLFVWA
                     IDEDAGPSTNRITLRVTDDALDAKSAARTFTVVVVAQVRVVINEIMYRPPPDGAEFVE
                     IHNTSTNTSWRLDGWRLTGTAFTFPAGTVLAPGGFLAVARDVGVFQAAYGSKASVIGN
                     YLNQLSPGGGTIRLLRPVAGGLEETVDQVTFLTRAPWPTTANGSGLSLQLIDPRQDNT
                     RVGNWSAVSGTSTNAPVNVVPMTASWRYWQHSSAPAPGWTNLVYDDSSWPNGGALLYV
                     ENDALPAPKTTPLTIGQMSYLFRTRFQFNGNPDGASLRVRTIIDDGLVLYLNGRAFFW
                     LGMDEGVIPARPDPAKRTIGNATEEGPFVRLVDNLRMGENVLAAEVHQNSIHSTDIAF
                     GVAVDVIEVRRESATPGYANSVRATLEPFPEVRINEVLADNQTGITDSRGDRDPWIEI
                     VNRQSGPALLDGWFLANSYANLGVWAFPKWAVLAGGHYQLVWADGESGETSAAEWHTD
                     FRLAPPTGVVVLSRLQNGQLAVVDFIEYTNLTADRSFGYMEPRVEASAPTVLAQPSPG
                     QPNLVDEPPRPRILSIAAGGSGEVTLVWETAPGWTYRIEARASIESGWWELLGQIVAT
                     SATASFTDFPPVDLDACYYRVLVAP"
     gene            4251..5276
                     /locus_tag="PKK20_05435"
     CDS             4251..5276
                     /locus_tag="PKK20_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNU99359.1"
                     /translation="MIIQCSAPIRICDLGGWTDTWFAGSGKVLNIAVFPSVEVTVVAG
                     RKRARDPQITIHAENYRDTYALKRRARTWTKHPLIEAGFRLFRLPSGLHLDVTIFSAA
                     PAGASTGTSASVSVAVLGALDLLGQRSLTSYELAAKAHYIESALLKLQSGIQDQLCAA
                     YGGINFIEMSRYPEASVSPVVLPEELTWELESRLLLIYLGRPHSSSAVHETVIRRLEE
                     SPRFRRYLEPLREAAAAGKAALLRCDLAAFGEAMSANTRAQQKLHPELVGDRARQVIA
                     LAREHGVSGYKVNGAGGAGGSITLLLKPGAREKHALIAAIEAANPDFRNLPIRLAPHG
                     LRRWRTG"
     gene            complement(5525..7003)
                     /locus_tag="PKK20_05440"
     CDS             complement(5525..7003)
                     /locus_tag="PKK20_05440"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNU99360.1"
                     /translation="MQLERLLAVYVLSLLCSLPVKGQSAPAPAFPPGRQTRPGWEPER
                     LPRILVGDSRRFLTDRGQPFVPMGVNYFRPGTGWAPQVWRQFDPDATRADFARLKELG
                     ANCVRVFLTFGSFYTEPGVLSEDGLAKFDRFLVLAEEAGLYVHPTGPDHWEGLPQWAR
                     GDRYAEESVLTSIETFWRLLARRYRERNVIFAYDLLNEPQIGWDSPAMRQRWNIWLAK
                     TYPTADQLKSAWGETNNPIELGSVPIPQRDTFPGRKLADYQRFREDLAEEWTRRQVEA
                     IKSADPLALVTVGLIQWSVPVLLAGPSQYSGFRPQRLAPLLDFMEVHFYPLARGFYEY
                     TEPEDEARNLAYLESVVREVARCGKPTVLAEFGWYGGGKPTIDEGKHPFASEEQQAQW
                     CRRAIETIEGLAGGWLNWGFYDHPGARDVSEFIGLLTADGELKAWGKAFREIATRFHD
                     HGLPAPKPLDRPNPDWDKLITDRKAVSEFREKYYQAFRASRQ"
     gene            complement(7218..8180)
                     /locus_tag="PKK20_05445"
     CDS             complement(7218..8180)
                     /locus_tag="PKK20_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNU99361.1"
                     /translation="MSDLPKPALIITHESDLDGLVSGVLLQRLARRLFGVEVPLLAYH
                     NHAWRQRPLPESAAWVCDLGFDKRLDRPGWLVVDHHEAEAVPQKARLIMDPGKSAGLL
                     AYDLCRQHGLESPALDRLVHLNNVADLFLVDDPEFDLANDYASLVKVYQFWNLHALIG
                     GELERLVDHPLLEVMAAKRRIENPLGFEWSRTHVSRLSDTVGYVDTIVGNSNLIVHQL
                     LNTQATPYTVLLTLLRQGNGTVVASLRSRNGEALKIAACLQGGGHPNAAGATLPRSVQ
                     RIPDAIAYLAKVLVPQPPASADAGGLESLFVGGAGAGTDRTRKT"
     gene            8404..9690
                     /locus_tag="PKK20_05450"
     CDS             8404..9690
                     /locus_tag="PKK20_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNU99362.1"
                     /translation="MSLSNPPLRLEAPAGQLLTPDQVHALVAQACPEPDFAGRRVLLI
                     VPDSTRTAPVGLLFKALHAALGRATRAFDVLVALGTHQPMTETAMCERLDISIADRQT
                     RFRGVRFFNHAWDDPQTLRDIGTIPSEEIHALSGGLFAMDVRVEVNRRLFDYDQIVIV
                     GPVFPHEVVGFSGGNKYLFPGVSGPRILNFFHWLGAVITTPKVIGHKWTPVRHVVDRA
                     AALVQVPKLCFAMVVEGEGLAGLFVGTPEAAWDAASDLSHALHIRYKDHPFHTILACA
                     PRMYDELWTAGKCMYKLEPVLADGGELIIYAPHIAEISATHGRHIAEVGYHCRDYFMR
                     QWDRFKDYPWGVLAHSTHVHGLGTFENGVEHPRARVTLATRIPESLCRSVNLGYRDPD
                     ALHAGDFVGREAEGILVVPKAGEILYRLKNPPPWAA"
BASE COUNT         1715 a         3162 c         3113 g         1762 t
ORIGIN      
        1 gccggtaaag aaatcgcccg ccccccgtcg cctctccggc tggcggctca ggggtgtcgg
       61 cttcaccttt ccgtccggcg tggtgctcgg cccaggcgcc ttcgctgtcg ttgcctcgaa
      121 tcgggacggc ttcgcggcgg cgtacggatt cggtgtgctg ccggtcgggg agtttcctgg
      181 caacctccag aacggcggtg agcgtttgcg cctggtcaga cccggggcca caccggagga
      241 ggatgaactc gtcgatgaag tccgctatgc gaacgagccg ccctggcccg ttcaggcgga
      301 cggctggggc ccctcgctgc aactgatcga cccgcgtcag gacaactggc gtgtcggcaa
      361 ctgggccgcc gcgcatccgg gcgacgcggt cgtggccacg cccggcaagc ccaattcggt
      421 tgctaagacc cttccgccgt tcccgccact gttcataaac gaagtgcagc cccagaatgt
      481 cagcggcccg gttgaccggt ttggcgatcg ggacccgtgg atcgagcttc acaacccggg
      541 cacgaatgcg atcgacctgg gcgggttctt cctgaccagc acatatacaa accgcacgga
      601 atgggccttc ccggacggcg cgcgcattgg tcccggcgag ttcttgctgg tgtgggccga
      661 cggcgagccg gtcgagtcga cggaggatga gctgcacaca cggtttcgcc tcgcgccccg
      721 cagcggcgcc gttgccctgg tgcgagaaca actcggtgcg ccggcggtca tcgacttcat
      781 caactaccaa tccatcccgg acgatctgtc cacaggctca tatccggacg gccagccgca
      841 atcgaggcag ttgtttcacc tgcctacgcc gggcggagca aatgatcccg gcacgccgcc
      901 tgtcgatgtc ttcatcaacg aatggatggc cagcaacacg ggcgtcgcgc tcgatccggc
      961 tgatggcgat gcggacgatt ggttcgagtt gtacaacgcc ggggcgcggg cggtcgatct
     1021 atcggcgtac acgctgaccg acaacctcga ggacccggcc aagttcgtga tcccgaacgg
     1081 cacggtgatt ccggcgggtg gctttctgtt ggtgtgggct gatgacgaga cggagcaatc
     1141 aggtcagggc cagttgcatg tcaacttcag gctctcggct gccggcgaat cgctcgggct
     1201 cttcaccccc gacgggcggg cggtggactc catcgcgttc ggctcccaga ccaacaatat
     1261 cagtgaaggc cgcttcccgg atgggatgat ccggccgccc tttgtcttca tgggccgccc
     1321 cacgccgggc gggcccaacg atttcccgac gctgaacgtg ccgcccacgc tcgatcccgt
     1381 gggcagccga actgtcgatg aaggacaaac ggtggccttc acggtctccg cgacagaccc
     1441 ggatgccggg caaacgctcg ttttcgcgct cttcggggcg ccgccggcgg cccagatcga
     1501 ttcgagaaca ggcgcgtttt cctggacgac gaccgaggcg gacgggccgg gcgcttatgc
     1561 attcaccgtg cgtgtcaccg atgacggcac cccgccgcgc tgggacgccg aagagatcac
     1621 aatcactgtc cgcgaggtga accagtcgcc cacgctcgat cccattgccg accagtcggt
     1681 cgacgagggg tctctgctga gcatccaggc cgtcgcgcgg gatgtcgatc aacccgcgca
     1741 atcccttcgg ttcagtttgg tcggtgtcgt ccccgaaggc gcgacgatcg acgcgctgac
     1801 cggagagttc tcgtggattc ccaccgagga ccagggagcg cagacttacg agatcaccgt
     1861 ccaggtcgct gacgacagcg tgccgccggg attggccacc cgcggctttc tggtagttgt
     1921 caatgaggtg gacaacgcgc cggtgttcga gccggtcagc ttgcagacgg tcgacgagtc
     1981 gagcccgttc gccctgcgcc tcagcgcaag ggacccggac tcgccgccgc gcgcggtgat
     2041 ctaccggctc gaatccggcc cgccgggtct gacactcgat cccgccaccg gtcaggtgaa
     2101 ctggacgcca gtcgaggcgc aagggccagg ctcgtacaac gtcgtcgtcc gcgcgatcga
     2161 gatcggaggg acacagacgt cgaccctctc ctttgcgatt gttgtgaacg aagtgaacga
     2221 gccgcccacg cttgcagcca tcgcggatcg tacggtggcc gaagggacgg ttttgtcgtt
     2281 catcaacaac gctgccgaca cggacctccc ccggcagacc ctccgattca gtctcgatcc
     2341 gggcgcgccg cccggagcga gtatcgatcc gcagagcggg ctgttcgtgt gggcgatcga
     2401 cgaggatgcg ggcccgagta cgaatcggat taccctgcgc gtgaccgatg acgccctcga
     2461 tgccaagtcc gccgcccgca ctttcacggt tgtcgtcgtg gcgcaggtgc gcgtcgtgat
     2521 caatgaaatc atgtatcgcc cgccgcccga cggcgccgag tttgtggaaa tccacaacac
     2581 gtcgacgaac acgagttggc gtttggatgg ctggcgattg acggggactg ccttcacatt
     2641 cccggcgggg accgtgctgg cgccgggcgg attcctcgct gtcgcgcgcg atgtcggcgt
     2701 ttttcaggcc gcgtacggat cgaaggcgtc cgtgatcggc aactacttga accaactgag
     2761 ccccggcggc ggcaccattc gacttttgcg tcccgtggcc ggcggcctcg aagagacggt
     2821 cgaccaggtg acattcctga cacgggcgcc gtggccaacg accgcgaacg ggtccggtct
     2881 gtcgctgcaa ttgatcgatc cgcgccagga caatacgcgc gttggcaact ggagtgctgt
     2941 gtcgggcacc agcaccaatg cccccgtcaa tgtcgtgccg atgaccgcat cgtggcgcta
     3001 ctggcagcac tcgtctgctc ccgcgcccgg gtggaccaac ctggtgtatg acgacagctc
     3061 atggcccaat ggcggcgcgc ttttgtatgt cgagaacgac gccctgccgg cgcccaagac
     3121 gacgccgctg accataggcc agatgagcta cttattccgg acccgctttc agtttaacgg
     3181 caacccggac ggcgcatcgc tccgggtgcg gaccatcatc gatgacggcc tggtgctcta
     3241 cctaaacggg cgggccttct tctggctggg gatggacgag ggcgtgattc ccgcgcgccc
     3301 ggaccccgca aaacgaacca ttggcaacgc caccgaggag gggccctttg tgcggcttgt
     3361 ggacaacttg cgcatgggtg agaacgtcct cgccgccgag gtgcatcaga acagcattca
     3421 cagcacggac atcgcattcg gcgtcgccgt cgatgtgatc gaagtccggc gtgagagcgc
     3481 aacaccaggt tatgccaact cggtgcgcgc gacgctcgag ccgttccccg aagtccggat
     3541 taacgaggtt cttgcggaca accaaacggg gatcaccgac agccgcgggg accgcgatcc
     3601 gtggatcgag atcgtgaatc ggcagtccgg tccggccctc ttggacgggt ggtttctcgc
     3661 caacagctac gccaacctcg gggtgtgggc ctttccgaaa tgggctgtcc tggcgggcgg
     3721 ccactatcaa ctcgtgtggg cggatggcga atccggcgaa accagcgccg ctgaatggca
     3781 caccgacttt cggctcgcgc cgcctacggg cgtagttgtg ttgagccgac ttcagaacgg
     3841 gcagcttgcg gtggtcgatt tcatcgagta cacaaatctg accgcggatc gctccttcgg
     3901 gtacatggag ccgcgggtcg aagcgagcgc gcccaccgtg cttgcacagc cttcgcccgg
     3961 ccagcccaac cttgtcgacg aaccgcctcg gcctcggatt ctgagcatcg ccgccggcgg
     4021 ctcgggcgag gtcacgttgg tgtgggaaac cgcgcctggc tggacctatc gcatcgaggc
     4081 gcgcgcgtcg atcgagagcg gttggtggga actgctgggc cagattgttg cgacctcagc
     4141 cacggcgagc ttcaccgatt tccctccggt cgatctcgat gcgtgttact atcgggttct
     4201 tgtggcgcct tgatgtcgcg atgtatttga cgcaccatcc gacgcgaaac atgatcatcc
     4261 aatgcagcgc acccattcga atctgcgatc tgggtggatg gaccgacacc tggtttgcgg
     4321 gctccggcaa ggtcctcaat atcgcggtct tcccgtcggt cgaggtgacg gttgtggctg
     4381 gccgaaagcg ggcgcgtgat ccccaaatca ccatccatgc cgagaactat cgcgacacct
     4441 atgcgctcaa acggcgggcg cgcacctgga ccaagcaccc gctgatcgag gccgggttcc
     4501 ggctgttccg cttgccgtcg gggctgcatt tggatgtgac gatattttcc gcggcgccgg
     4561 ccggggcctc gaccgggaca tctgcatcgg tgtccgttgc ggtattgggg gcgctcgatc
     4621 tgctgggcca gcgctcgctg accagctacg aattggccgc caaagcccat tacatcgagt
     4681 ccgccctgct caagttgcag tccggcatcc aggaccagtt gtgcgccgct tatggcggca
     4741 tcaatttcat cgagatgagc cgttacccgg aggcatcggt ctcgcccgtt gtcctgcccg
     4801 aagagctgac ctgggaactc gaatcccggc tgcttctgat ctacctcggg cggccgcact
     4861 cgtcgtccgc ggtccacgag acggtgatcc ggcggctcga ggagtcgccg cggttcagac
     4921 gctatctcga gccgttgcgc gaggcggcag cggcgggcaa ggcggctttg ttgcgttgtg
     4981 atctggccgc gtttggcgag gcgatgtccg ccaacactcg ggcgcaacag aagctgcatc
     5041 cggaactggt tggcgaccgc gcccgccagg tgatcgccct ggcccgtgaa cacggggtct
     5101 cgggttacaa agtcaacggg gccggaggcg ccggaggctc gatcacgctc ctgctcaagc
     5161 ccggcgcgcg agagaagcac gcgctcattg cggctatcga ggccgcaaat cccgatttcc
     5221 gcaacctccc catccgcctg gcccctcacg gcctgcgccg ctggcggacc ggttgacccg
     5281 atctacccct aaaacacacg gggccgaact tccaagttcg gcccacaaca acgagaactg
     5341 cggacacgat acgcgcagag ggttttcgcg ccagtcacca atactggtga caccagtccg
     5401 ccagtgcgtc cggaggttgt tggtgggaat cgaatatgcg cggcgtgtgc tccgttcgcg
     5461 cacacgaggg cgttggagcg gggacgtcct cgtccccggc gatgagagtt gcagacccta
     5521 agattcactg tcgagaggct ctgaacgcct ggtagtactt ctcccgaaac tcgctcaccg
     5581 ccttgcggtc ggtgatgagt ttgtcccaat ccggattcgg acgatcgagc ggcttgggtg
     5641 cggggagccc gtgatcgtgg aatcgcgtgg cgatttcgcg gaacgccttt ccccaggcct
     5701 tcaattcgcc gtccgcggtg agcaagccga tgaactcgct cacgtcgcgg gctcccgggt
     5761 ggtcgtagaa accccaattc aaccatccgc ccgccagccc ctcgatggtc tcgattgcgc
     5821 gacgacacca ctgggcctgt tgctcttccg aggcgaaagg gtgtttgccc tcgtcgatcg
     5881 tcggcttgcc gcctccgtac cacccgaact cggccaacac ggtcggtttg ccgcaccgtg
     5941 cgacttcgcg aaccacgctt tcgagatagg cgaggttacg cgcttcgtcc tccggctcgg
     6001 tgtattcgta gaatccacgg gccaacggat agaaatgaac ctccataaag tcgagcagcg
     6061 gggccaggcg ttgcggacgg aaccccgagt actgcgacgg ccccgccagg aggaccggga
     6121 ccgaccattg gatcaaaccg accgtgacca gcgccagcgg atcggccgac ttgatcgcct
     6181 cgacctggcg ccgggtccat tcctcggcaa ggtcctcccg gaaacgctgg tagtcggcca
     6241 gcttccgtcc cgggaacgtg tcacgctgcg ggattggcac gctgccgagt tcgatgggat
     6301 tgttggtttc gccccaagcg gacttgagtt ggtcggcggt cgggtaggtt ttcgccagcc
     6361 aaatgttcca gcgctggcgc atcgccggtg aatcccagcc gatctggggt tcattgagca
     6421 ggtcgtaggc gaagatcacg ttgcgctcgc gatatcttcg agcgagcaag cgccaaaagg
     6481 tctcgatcga ggtcaagacc gactcctcgg catagcggtc tccgcgtgcc cattgcggaa
     6541 gaccctccca atggtccggc ccggtcggat gcacatagag ccccgcctcc tccgcaagaa
     6601 ccaggaaccg atcgaacttg gccaggccat cctccgagag cacgcccggt tccgtgtaga
     6661 aggaaccgaa tgtgagaaac acccgcacgc agttcgcgcc gagttccttg agtcgcgcga
     6721 agtccgcccg cgtggcgtcg ggatcgaatt gtctccagac ttgcggcgcc catcctgtgc
     6781 cgggccgaaa gtagttcacg cccatcggaa caaaaggctg accacggtcg gtcaggaaac
     6841 gccggctgtc gcccacgaga atccgcggca gacgttccgg ttcccagccc ggacgtgttt
     6901 gtctgcccgg cggaaaggcg ggtgcaggcg cggactgacc cttgacgggc agcgagcaca
     6961 ggagactcaa cacataaacc gcgagaagcc gctcgagttg catggccgca agctatcagg
     7021 ccggtccgat gccgcaaggc aatcgtctgc tcgcaaggcg cttaaccacg gtcattgaac
     7081 tgcaaaggat tgcatccaaa acgcactgat atcgtgtcgc gatcgcgaca aggaaactgc
     7141 acagtgcgct ggtgacggtg ctgggaagga accaggaggg cagggcagga gattcgacca
     7201 acgccggatc gaacctctca agtcttcctg gtccggtctg tgccggcgcc ggcccccccg
     7261 acaaagaggc tttcgagtcc gccggcgtcc gcggaggcag gcggctgggg aacgagcact
     7321 ttggccaggt acgcgatggc atcggggatg cgctgcacag agcgcgggag cgtcgcgcca
     7381 gccgcattgg gatgaccgcc tccctggagg caggcggcta ttttgagggc ctcgccgttg
     7441 cggctgcgca ggcttgcgac caccgttccg ttgccctggc gcaggagggt caagagcacc
     7501 gtgtagggcg tggcctgggt gttgaggagt tggtgcacga tcaggttgct gttgccgacg
     7561 attgtgtcga cgtacccgac ggtgtcgctc aggcggctga catgggtgcg actccactcg
     7621 aagccgagcg ggttctcgat gcgacgcttt gcggccatga cttcgagcag cggatgatcc
     7681 accaggcgtt cgagttcgcc gccgatcagg gcgtgcagat tccagaactg gtagaccttg
     7741 acgaggctgg cgtagtcgtt cgccagatcg aattccggat cgtccaccag gaacaggtcg
     7801 gcaacgttgt tgagatgcac cagtcgatcg agggccggcg attcgagccc gtgctgccgg
     7861 caaagatcgt acgcgagaag gcccgccgac ttgccggggt ccataatcag ccgtgccttc
     7921 tgcggaacgg cctcggcctc atgatgatcc acgaccagcc agcccggtcg gtcgagtcgc
     7981 ttgtcgaacc cgaggtcgca gacccacgcg gccgattcgg gcagggggcg ttgccgccac
     8041 gcatggttgt gataggccag cagcggcacc tcgacaccaa aaaggcgccg agccaggcgc
     8101 tgcagcaaaa cgcctgagac caagccgtcc aggtcgcttt cgtgcgtgat gatcaatgcg
     8161 ggcttcggca agtcactcat gtcgcgggcg aacctagccc ggaactctcc cgaccggcaa
     8221 gggggatcac gccggatgga tcacacaccg agcctcgcgc caactgtgag gggcaacccc
     8281 gcatattgat gccacgcagt gcccagggcg gtcacatctc tcaagatgtc agccatgcgg
     8341 actggaaaaa cccccactct tttgccttcc ccgacgctgg ccgacatcgc acagtcgtgc
     8401 ccaatgagcc tgtccaatcc gcctctccga ctcgaagcgc ccgccggtca actgctgacg
     8461 cccgaccagg tccatgccct ggtcgcccag gcatgtccgg agccggactt cgcgggccgg
     8521 cgcgttctgc tcattgtccc ggacagcacg cgaacggccc cggtcggcct gctgttcaag
     8581 gcgctccacg cggccctggg gcgcgccact cgggcattcg acgtgctcgt ggcgctgggc
     8641 acgcaccagc cgatgaccga gaccgccatg tgcgagcgac tcgatatctc gatcgccgat
     8701 cgccagacgc gttttcgggg cgtgcgcttc ttcaaccatg catgggacga tccccagacg
     8761 ctgcgcgaca tcggcaccat cccgtccgag gagattcatg cgctcagcgg cggcctgttc
     8821 gccatggacg tgagggtcga agtcaaccgc cggttgttcg actacgacca gatcgtcatc
     8881 gtcggtccgg tctttccgca cgaagtcgtc ggattctccg gcggcaacaa gtacctgttc
     8941 cctggcgtga gcggcccgcg catcctcaac ttcttccact ggcttggggc ggtgatcacc
     9001 acgcccaaag tcattggcca caagtggacg ccggtgcggc atgtggtgga tcgtgccgcc
     9061 gcgttggtac aggttcccaa gctctgcttt gcgatggtgg tcgagggcga aggcctggcc
     9121 ggtctgtttg tcggcacgcc cgaggccgcc tgggacgccg ccagcgatct ctcccatgcc
     9181 cttcacatcc gctacaaaga ccaccccttt cataccatcc tggcttgcgc gccccggatg
     9241 tacgacgagc tttggaccgc cggcaagtgc atgtacaaac tcgaaccggt gcttgcggac
     9301 ggcggcgaac tcatcatcta cgcgccccac atcgccgaaa tttccgccac ccacggacgc
     9361 cacatcgccg aggtcggcta tcattgccgc gactacttca tgcggcagtg ggatcggttc
     9421 aaggactatc cctggggcgt tctcgcccac tcgactcacg tgcacgggtt gggcacattc
     9481 gaaaacggcg tcgaacatcc gcgcgcacgt gtcaccctcg ccacacgcat ccccgagtcc
     9541 ctctgccgct cagtcaacct cggttaccgc gatccggacg cccttcatgc cggggatttc
     9601 gtcggacgcg aggccgaggg cattctcgtg gttccgaagg ctggcgaaat cctctatcgg
     9661 ctcaagaacc ctccgccctg ggcggcctga tgccggcatt gatcggagat cgaagacggg
     9721 agatcgaaga tgggggggat ggtgtgtcgg tg
//
LOCUS       DAOQRO010000101         9749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6322_length_9749_cov_5.338869, whole genome shotgun sequence.
ACCESSION   DAOQRO010000101 DAOQRO010000000
VERSION     DAOQRO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6322_length_9749_cov_5.338869"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..2274)
                     /locus_tag="PKK20_05455"
     CDS             complement(52..2274)
                     /locus_tag="PKK20_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99363.1"
                     /translation="MRCRLVLLLAAVLATLGVAHPGTPVMRVVQSSAASSNLLRNPGF
                     ELGTSNMLTSWSPGPQGLRVAPGEGRTNSQALACRAPNTAGWYGASQTLSLNRTNCVP
                     VVVRGWSRAAGVSGSADSDYSLYVDIVYQDGSPLWGQTGNFTTGTHDWEAREFVILPE
                     KPIRSLTLYCLFRNHSGEVWFDDVSVAEISAPAGAFLFQGTPVTVETLASPSGQPTTY
                     STGDGLELMLTGGRVARLCVDGRNLTGSAPSGFLARDVATNSDVFSFEEGACPDLNLL
                     LRATIITNADHLAVTGTVVDTTGTDRAIMLLFALPLDAVGWSWGHDIREQRPVAAPEE
                     LSNTVGVNCGANGRHSVYPLACVSDGRSGLALAVDMGKPAQYRLAYHAGTKQLLVAYD
                     LGLVPETARFPSGAEFRFVLFRFDPRWGFRGAWEKYTAMFPAYFEVRSKDQGIWMPFT
                     DVSTVSGWQDFGFRYHEGNNNVPFDDANGILSFRYTEPMTWWMSMDPALPRTMATALE
                     VRDAYAAGAPGTRQRMAIVSRSAGMFDSTGQPCLLFRNEPWANGAVWSLNPNPLLPAD
                     PNAATVHWNASIRESLYGPNAKGNLDGEYLDSLEGYVTADLNFRREHFASSTVPLTFD
                     TTTARPALWKGLAVFEFTRWISDDLHQFGKLCFANGVPYRFSFLCPWLDVMGTETDWL
                     SNGRYAPSSHGQMSLWRTLSGKKPYLLLMNTDYSSFGTNRVERYFQRSLFYGIYPSMP
                     "
     gene            complement(2695..3927)
                     /gene="amrS"
                     /locus_tag="PKK20_05460"
     CDS             complement(2695..3927)
                     /gene="amrS"
                     /locus_tag="PKK20_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNU99364.1"
                     /translation="MEVGALGCAGLFGADSIFSFLRRWKQDAEPADVFKGDAPSEAVW
                     DLWRKRGWVREAQHYLKLDRNVQCKICPNNCLLEPGDRSHCRNKVNRGGTLYTLAYGN
                     PCTFHVDPIEKKPLFHFLPGSRSFSLATSGCVFRCLNCQNWEISQKKPEETKDPQGGE
                     FRLRPPVPPALTLGQIRRLSLFPEDLVAVAQDLDCPSISYTYSEPTAYYEYAYDSCRL
                     ARAGGLRNVLVSCGSIEERPLRDLGQYVDAMHVDLKGFSDDIYRKLNSGRLDPILRTL
                     TTLKSLGVWVEVINLVVPTYTDDLDMIRRMCGWLVAHLGVDQPLHFSRFHPQHKLTHL
                     APTPVDFLVRARDTARREGLRYVYIGNVSGLADAQTTFCPNCSRPLIQRDIFAVAALD
                     VVDGKCRFCNTPIAGVWA"
     gene            complement(3942..4229)
                     /locus_tag="PKK20_05465"
     CDS             complement(3942..4229)
                     /locus_tag="PKK20_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99365.1"
                     /translation="MHKLNPILLKAVRWTGWPLLPLVLAFLATGYAISGRYGLGHLLD
                     QQAALTLHKLLHAPLLLLLLVHSIPAIYLALQRWNRTRKRPAVRTHGHPPG"
     gene            complement(4202..6472)
                     /locus_tag="PKK20_05470"
     CDS             complement(4202..6472)
                     /locus_tag="PKK20_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99366.1"
                     /translation="MNLAPVRWAVAGLGFSSAVTQLVLLRELLATFQGNELILGLLLG
                     TWLLLTGIGTQLWRAGSMGESARLVRRNSESDGRRAAEDESVVHPADCSPRAPSVASL
                     PGLAALQIWIALIPFAQVLAVRLGRDAVFLRGATLGLNQTVWSSLLVLAPYCLASGVF
                     LTLASRAAGETNAARGVSTAYLADCLGSVIGGALFTFALVRWLDHLALLVVPSTVNLL
                     LAALTLRRIARNRIAVLPLALAASLVACVAWLDPDALTTAWQSRPDRVVFRAQSPYGR
                     LVVTESAGQLNFIENGLPLLSSRQIERAEEIAHIAMAQRPSARNVLLISGALSGAAGE
                     ILKYGVTNLTCVELDPAILEAGRRLLPERLTDRRLRVVNTDGRRYVQRGDGPYDLAIV
                     DMPAPATAQLNRYYTAEFFAELGRVLESDGAVVFGLGQYDNYVSPELARMLASTARTL
                     RLRFRTVLLLPAAKVWFLASDGPLTPSIAARLRQAGVSAQWMRPSYLDAMLTPERLAA
                     VASAAELPAPVNSDFRPVLYFLHLRHWLSQFDVWLGAAAIVLGFSLAVYIARLSASAF
                     AVFSGGFAGSGLEVALLLGVQVLYGSLYQQLGLIVTVFMAGLAVGAWLGGRQGSRADL
                     RRLASVAFALAIYGILMPWMLTALAQMSPAGMPPWFGQVLLALLTAGLAVLVGLEFPL
                     ACRVERADVGTLASRLFCADFVGACVGAILAGALLIPILGVTAACGVAASLNALAGLR
                     LLWRGRTSCTSSIQSS"
     gene            complement(6469..8076)
                     /gene="amrB"
                     /locus_tag="PKK20_05475"
     CDS             complement(6469..8076)
                     /gene="amrB"
                     /locus_tag="PKK20_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNU99367.1"
                     /translation="MKSLLDVRVPVRARRRLAVAACIGSLSAVWHSPQILGASSAPAD
                     ASNQPARDAAFAPARIREPAVAGLFYPAEAGALSSAVDRLLARARPEPVPNLKALICP
                     HAGYQFSGPTAAHGYKLLGGHDWRTVVVLAPSHYARFKGASVVGASAFRTPLGDVRVS
                     SLAARLARTPPFGPERPCPVQRPAWAGQSSRPSPARGQDTPHSWEHADEVQVPFLQRA
                     LKDFEIVTVILGEVDAAEVARGLAGHLDEHTLVVASSDLSHYHPYDQAKALDTRCVKA
                     VCDLDLDGMLEQEACGRAPILALMHLARQKGWKAKLMDYRNSGDTGGDKSGVVGYASI
                     AFTGEAEGSNPYSIEERRRLIELARQAVREAVTTGRSPSVNAEDFPPSLREPKGSFVT
                     LTQQGALRGCIGNLRATEPLYASIISNARSAATRDYRFPPVRKEELDSIEFEISILSD
                     PKPLDFTSPEDLLAKLQPHRDGVVLEIGSATSTFLPQVWRQLPDKVQFLSALAKKADR
                     GPDDWRKPGTRVLVYRVEAFKESELKQ"
     gene            complement(8235..9506)
                     /locus_tag="PKK20_05480"
     CDS             complement(8235..9506)
                     /locus_tag="PKK20_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNU99368.1"
                     /translation="MKFNQWTLGLAAVGLVSLGTAAQAEEAQNPILTALSSTTISGYV
                     NTSAILRFGPSLPNGDYSTPGRTFDANNKFNGFNLDVVKLQIEKPLDESQWSAGYQVG
                     LIFGPDANTYGTDSLGGDSDFGIKDAAVTLRAPVGNGLDFRIGYWESPIGYEVFDAGN
                     NPNYSRSYAYAIEPKQFTGVLASYRVSEILSLSGGIANRGWEVGGTWDHPVRYYADNA
                     INKRSGGKTILTYLGSVALTAPESAGFLEGATLYGGIIDSGMAHGPDVLNLYVGGTMP
                     TPVKGLLLGLSYDYRGSSGDSGYDSSYANAFGAYLSYQLTEKLKFNARGEYVRGSNGT
                     WYSSGDWDLDNTDDNGNPDPLLVMDSEKNELIGVTATLEYSLWANVLTRLEFRWDHDC
                     NGNGIFNDYIPGVSKGTDDAFQLALNVIYKF"
BASE COUNT         1846 a         2885 c         3327 g         1691 t
ORIGIN      
        1 ctccagtttc ggcacgaacc gtgtgtaacg gtattttcag cgcagcctct tctatggcat
       61 cgacgggtag atgccataga agaggctgcg ctgaaaatac cgttcgacac ggttcgtgcc
      121 gaaactggag tagtcggtgt tcatcaggag aaggtagggc ttcttgccgg agagggtgcg
      181 ccacagcgac atttggccgt gggaagacgg cgcgtatcgg ccattggaaa gccagtcggt
      241 ttcggtgccc atgacatcga gccacgggca taggaagctg aatcggtagg gaaccccgtt
      301 ggcgaagcag agtttgccga attgatggag atcgtcgctg atccagcggg tgaactcgaa
      361 gacggccagc cctttccaga gggccggacg ggcggttgtc gtgtcgaacg tcaatgggac
      421 cgtgctggaa gcgaagtgtt cgcgacggaa attcaggtcg gctgtgacat atccctcgag
      481 tgagtcgagg tactcgccat cgagattgcc cttggcgttc ggaccgtaga gcgattcgcg
      541 gatgctggca ttccagtgga cagtggcggc gttggggtcg gcggggagaa gggggttggg
      601 gttgaggctc cagacggcgc cgttggccca cggctcattg cgaaagagaa ggcaaggctg
      661 gccggtgcta tcgaacatcc cggcgctgcg cgagacgatc gccatccgct gccgggtgcc
      721 gggggcgccc gcggcgtagg cgtcccgaac ctcgagcgcc gtagccattg tgcgcggaag
      781 tgccgggtcc atggacatcc accatgtcat gggttccgtg tagcggaaac tcaggatgcc
      841 gttcgcatcg tcaaagggga cgttgttgtt gccttcgtga tagcggaagc cgaaatcctg
      901 ccagccgctg actgtgctca cgtcggtgaa aggcatccag attccctggt ctttcgaacg
      961 cacctcgaag tatgcgggga acatggctgt gtacttctcc cacgcgccgc ggaaacccca
     1021 gcggggatcg aatcgaaaga ggacgaatcg gaactccgcg ccggagggga accgggcggt
     1081 ttcgggcacc aacccgaggt cgtaggcgac aagcaattgt ttcgtgccgg catggtaggc
     1141 aaggcggtac tgcgctggct tccccatatc gacggcaagg gcgagtccgg accgtccatc
     1201 gctgacgcag gccaggggat aaacggaatg gcgtccattg gcgccgcagt tcacaccgac
     1261 ggtgttgctg agttcctcgg gggcggcaac gggtctttgt tcgcgaatat cgtggcccca
     1321 gctccagccg acagcgtcca gggggagggc aaacaggagc atgatggcgc ggtccgtgcc
     1381 ggtggtatcg accacggtcc ctgtcacggc cagatgatcg gcattggtga tgatcgtggc
     1441 gcggaggagc agattcagat ccggacaagc gccttcttcg aaggaaaaga cgtccgagtt
     1501 tgtggccacg tcgcgcgcga ggaaccccga gggggcgctg ccggtgaggt tgcgcccgtc
     1561 aacgcacagg cgggcgacgc gtccgccggt gagcatgagt tcgaggccgt cgcctgtgga
     1621 ataggtggtt ggctgtcccg agggcgaggc cagggtttcg acggtcacgg gggtgccttg
     1681 gaacaggaag gcgccggcgg gggcgctgat ctcggccacg gagacgtcgt cgaaccagac
     1741 ctcgccggag tggtttcgaa agagacagta gagggtgagg gaccggatcg gtttctcggg
     1801 caggatgacg aactcgcgcg cttcccagtc gtgggtgcct gtggtgaagt tgccggtctg
     1861 gccccagagc ggactgccgt cctggtagac gatgtcgacg tagagggaat agtcgctgtc
     1921 ggcgctgcca ctgacccctg cggcgcggct ccagccccga acgacgacgg ggacgcagtt
     1981 ggtgcggttg aggctcagcg tttggctggc gccataccag ccggcggtat ttggggctcg
     2041 gcacgcgagg gcctgcgagt tggtgcggcc ttcgccgggg gcaacgcgca gcccctgggg
     2101 gccgggggac catgaggtga gcatgttcga agtgcccagt tcgaagccgg ggttccggag
     2161 gaggttcgag cttgcggctg aggattggac gacgcgcatg accggcgtgc ccggatgcgc
     2221 gacgccgaga gttgcgagca cggcagcgag aagaaggaca agacgacagc gcatgacgct
     2281 ttgtctgata gtccattcgg cggttttggt aaaggccgaa ggcggacgac catcaagggg
     2341 gtttttggcg agggacaaga ccctgcctgc cgcctgcctg ccgcctgcct gcctgccggc
     2401 aggcagggca ggcagggcct cgtcgcccca caccccttca caagcatcct tcggcggagg
     2461 gcaagccgtc cccctccaag gttcggttca tggaaagtat tgacattcaa cggtttaatg
     2521 cgcattggac ccatgaaccg gggaacgttc aacgcccaac ttccaacgct caacgttcaa
     2581 cgaagcggtt cgttgaaagt tggatgttga acagcatcaa cgacgccttc ttcggcactt
     2641 ggcgtcactt ccccaagacg atcatcgact gactcttccg gttcaatagg cagcttacgc
     2701 ccagactccg gcgatggggg tgttgcagaa gcggcacttg ccgtccacca cgtccagcgc
     2761 ggcgacggcg aagatgtcgc gctgaatgag agggcggctg cagtttgggc agaaggtggt
     2821 ttgagcgtcg gcgaggccgg acacattccc gatgtagaca tagcgcagcc cctcacgccg
     2881 ggcggtgtcg cgggcccgga cgaggaaatc gacgggcgtc ggggcgagat gagtgagctt
     2941 gtgctgggga tggaatcgag agaagtggag gggctgatcc acacccagat gcgcgacaag
     3001 ccacccgcac atgcgccgga tcatgtcgag atcgtcggtg taggtcggga cgacgaggtt
     3061 gatgacctcg acccatacgc ccaggctctt gagcgtggtc agggtgcgca ggatgggatc
     3121 gaggcgcccg gagttgagct tgcgatagat gtcgtcgctg aatcccttga gatccacatg
     3181 catcgcgtcg acgtactggc ccaggtcgcg cagcgggcgt tcctcgatcg agccgcaact
     3241 gacgagcacg ttgcgcaacc cgccggcgcg cgccagccgg cacgagtcgt acgcatattc
     3301 gtagtacgca gtcggctccg agtaggtata ggagatcgac ggacaatcga gatcctgcgc
     3361 caccgccacc agatcctcgg ggaagaggct caggcgccga atctggccca gagtgagcgc
     3421 cggcggcaca ggcgggcgca gtcggaattc gcccccctgc ggatctttgg tctcctcagg
     3481 tttcttctgc gagatttccc agttctggca gttgaggcag cgaaacacgc acccggacgt
     3541 ggcgagtgaa aacgagcggc tgcccgggag gaaatggaac agcggtttct tctcgatggg
     3601 atcgacgtgg aacgtacacg ggttgccgta ggcaagcgta tagagcgtcc cgccccggtt
     3661 gaccttgttg cggcagtggc ttcgatcgcc cggttcgagc aggcagttgt tgggacagat
     3721 cttgcattgc acgttgcggt ccagcttgag gtagtgctgc gcctcccgga cccagccgcg
     3781 tttccgccag aggtcccaca ccgcctcgga gggggcgtcg cccttgaaaa catccgcggg
     3841 ttcggcatcc tgcttccacc tccgaagaaa cgagaaaatc gagtcggcgc cgaacagtcc
     3901 ggcgcatccg agggcgccga cttccaaggc tcgccggcgg gtcagccggg cgggtgtccg
     3961 tgggtgcgaa cggcgggtcg ttttcgcgtt ctgttccatc gctggagggc aagatagatc
     4021 gccgggatgg aatgtacaag gagcagcagg agcagggggg cgtggagaag cttgtgcagc
     4081 gtcagcgcgg cctgctgatc gagcaagtgc cccaatccgt atcggccgct gatggcatat
     4141 ccggtcgcca ggaaggccag aaccaggggc agcagcggcc aaccagtcca gcgaaccgct
     4201 ttcaagagga ttggattgag cttgtgcatg atgttcttcc gcgccagagc agacgcaggc
     4261 ccgccagcgc attgaggctc gccgccacac cgcacgccgc ggtcacgccg aggatgggaa
     4321 taagcagggc gccggcgaga atcgctccca cgcacgcgcc gacgaaatcc gcgcagaaaa
     4381 gcctcgaggc caaggtgccc acatccgccc gttcgacgcg gcatgccagc gggaattcga
     4441 gtccgaccag gacggccaga cctgccgtga ggagggcgag gaggacctgt ccgaaccagg
     4501 gtggcatccc ggcgggactc atttgggcaa gagcggtcag catccaaggc atcaggatgc
     4561 cgtagatggc aagcgcgaag gccactgacg cgagtcggcg gaggtctgcc cgagagcctt
     4621 gccgtccgcc aagccaggcg cccacggcca gaccggccat gaacacagtg acgatcagac
     4681 cgagttgttg gtacaaagac ccgtagagga cctgcacgcc gagcaggagg gcgacctcga
     4741 gacccgagcc ggcgaacccc ccgctgaaca ccgcgaaagc cgaggcgctc agccgagcga
     4801 tatagactgc caggctgaaa ccgagcacaa tcgccgccgc gccgagccag acgtcgaatt
     4861 ggctcaacca gtggcggagg tgcagaaagt agaggacagg gcggaaatcg gagtttacgg
     4921 gcgcaggcag ctccgcggcg cttgcgactg ccgccaatcg ttccggagtg agcatggcat
     4981 cgagatacga aggcctcatc cattgggcgg atacgcccgc ctggcgcagc cgcgcggcga
     5041 tggacggcgt cagcggaccg tctgaagcta ggaaccagac cttggcggcc ggcaggagga
     5101 ggaccgtgcg gaacctcagg cgcagcgtgc gcgccgtcga ggcgagcatc cgggcgagtt
     5161 ccgggctgac gtagttgtcg tactggccga gtccgaacac cacggcgccg tccgattcga
     5221 gcacgcgccc gagttcggcg aagaactcgg cggtgtagta gcgattcaac tgcgcagttg
     5281 ccggggccgg catgtccact atcgcgagat catacgggcc gtccccacgc tgaacatagc
     5341 gccgaccgtc ggtgttgacg acgcgcaggc gccggtccgt cagtcgctcg ggcagcaggc
     5401 ggcgtccggc ctcgagaatg gccgggtcga gttcgacgca cgtcaggttg gtcacgccgt
     5461 atttgaggat ttcacccgcg gcgccggaga gcgcgcccga gatgagcaac acgttgcggg
     5521 cggaggggcg ttgggccatt gcgatgtgcg cgatttcctc cgcgcgctcg atctggcgac
     5581 tcgagagcag cggcaatccg ttttcgatga agttcaattg gccggcggat tcggtcacca
     5641 ccaggcgtcc gtacggcgac tgggcgcgga acacgacccg atcgggccgg gattgccagg
     5701 cggtggtgag ggcgtcggga tcgagccagg cgacgcaggc gaccagagac gccgccagcg
     5761 cgaggggcag cacagcgatt cggtttctgg cgatccggcg aagagtcagc gctgccagga
     5821 gcaggttgac tgtggatggc accacaagca gggcgaggtg atccagccac cggaccaaag
     5881 cgaatgtgaa caacgcgccg ccgatcacgc tgcccaggca atcggccaga tacgccgtcg
     5941 acacgcctcg agccgcattc gtttcgccgg ccgcccggga cgccagtgtg agaaagacgc
     6001 ccgaggcgag acagtacggg gcgaggacaa gcaagctgct ccagaccgtc tgattcaggc
     6061 ccagcgtcgc gccgcggagg aagacggcat cgcgtccgag ccggactgcg agcacctggg
     6121 cgaaggggat taaggcaatc cagatctgga gagcggcaag gcctgggaga ctcgcaaccg
     6181 atggcgcgcg cggagagcaa tccgcaggat gcacaacact ttcatcctca gccgcccttc
     6241 gcccatcgct ctccgagttg cggcggacaa gccgggcact ctctcccatc gaacctgccc
     6301 gccaaagctg ggtcccgatg ccggtgagca gcagccaggt gcccaggagc agtcccagaa
     6361 tcagctcatt gccctggaac gtcgcgagca gttcccgcag caggaccagt tgtgtgacag
     6421 cactcgagaa tcccagcccg gccacggccc agcggacggg cgcaaggttc actgcttcaa
     6481 ctccgattcc ttgaaggcct cgacgcggta gacgaggacg cgcgtgccgg gctttctcca
     6541 gtcatcgggt ccgcggtcgg ccttctttgc cagggctgag aggaactgga ctttgtccgg
     6601 gagttggcgc cacacctggg gcaggaacgt ggatgtagcg gagccgattt cgagcacgac
     6661 gccgtcgcgg tgcggctgga gtttggcgag caaatcctcc ggagacgtga agtccagcgg
     6721 ctttgggtcg ctcaggatgc tgatctcgaa ttcgatcgag tcgagttctt ccttccgcac
     6781 cggggggaac cggtaatcgc gcgtggctgc gctgcgggcg ttgctgatga tcgaggcata
     6841 gagcggctca gtggcgcgga ggttgccgat gcagcctcgc aatgcgccct gttgtgtgag
     6901 cgtgacgaac gaccctttgg gttcgcgcag gctggggggg aagtcctctg cgttcacgga
     6961 cggcgagcgt ccggttgtga ccgcctcgcg caccgcctgg cgcgccaact cgatcaggcg
     7021 tcggcgttcc tcgatcgagt aggggttcga gccctcggct tcgccggtga aagcaatcga
     7081 ggcgtagccc accacgccgc tcttgtcgcc cccggtgtca ccgctgttgc ggtagtccat
     7141 gagcttggcc ttccatccct tttgacgggc caggtgcatc agtgccagga tcggcgcccg
     7201 gccgcatgct tcctgctcga gcatgccatc gaggtccagg tcgcaaacgg ccttcacaca
     7261 ccgtgtgtcg agcgctttgg cttggtcata gggatgataa tggctcaggt cggaactggc
     7321 taccaccaaa gtgtgctcat cgagatgccc ggccagaccg cgcgcgacct cggcagcatc
     7381 cacctcgccc aggatcactg tcacaatctc gaagtccttc aacgccctct gaagaaatgg
     7441 cacctggacc tcgtccgcat gttcccagga gtgcggcgta tcctggccgc gcgcagggga
     7501 tggccggctc gattgaccag cccaagcagg ccgttgcacg gggcagggcc tttccgggcc
     7561 gaaggggggc gtcctggcca gtcgtgcggc caaactcgac acgcggacat ctcccagcgg
     7621 tgtgcgaaac gcgcttgccc ccaccacgct tgctcctttg aaacgggcgt agtggctggg
     7681 cgccagcaca accacagtcc gccagtcgtg tccgcccaga agcttgtagc cgtgggcggc
     7741 agtcggcccc gagaattgat agcccgcgtg cgggcaaatg agcgccttga gattcggcac
     7801 aggctcgggc cgtgcgcgcg caaggaggcg atcgacggcc gacgacagcg ccccggcttc
     7861 cgcgggatag aacaatccgg caaccgcagg ttcgcgaatg cgggcgggcg cgaacgcagc
     7921 gtcgcgtgcc ggctggttgg acgcatcggc cggagcggat gacgcgccga gaatttgcgg
     7981 gctgtgccag acggcgctca aagagccgat gcatgccgcg actgcgagcc gccgtcgggc
     8041 gcggaccggc acccgaacgt cgaggagaga tttcatgcag tcaagtgtaa cacgggtgcg
     8101 cgatcccgat caaccaggcg tctgcagtcc ggtcgcgcgc gagtgcttcg acgatggaat
     8161 ctgcaacggg cgggtctcga gaaaaaaaaa acccctcccg gcccagccgg gagggggtgc
     8221 ggagcggaaa ggctttagaa cttgtagatg acgttcagag ccagttggaa ggcgtcgtcg
     8281 gttcccttgc tcacgcccgg aatgtaatcg ttgaagatac cattgccgtt gcagtcatgg
     8341 tcccaacgga actcgagtcg cgtgagcaca ttggcccaga ggctgtattc gagggtcgct
     8401 gtgacaccga tcaactcgtt cttctcgctg tccatgacga ggagcggatc cgggttgccg
     8461 ttgtcgtccg tattgtcgag gtcccagtcg ccggaggagt accaggtgcc gttcgatccg
     8521 cggacgtact cgccgcgggc gttgaacttg agcttctcgg tcaactgata ggagaggtaa
     8581 gcgccgaacg cgtttgcata cgacgagtcg tatccgctgt ccccgctgct cccccggtag
     8641 tcataagaca agccgagcaa caaacccttg accggcgtgg gcatcgtgcc gccgacgtac
     8701 aggttgagga cgtcgggtcc gtgcgccatg ccgctgtcga tgatgccgcc gtagagggtg
     8761 gcaccttcga ggaagcccgc gctctccgga gccgtgaggg cgacggagcc gaggtaggtc
     8821 aggatggtct tgccgccgct gcgcttgttg atcgcgttat cggcgtagta gcggacggga
     8881 tgatcccatg tgccgccgac ttcccaaccg cggttggcaa tgccgccgga gaggctcagg
     8941 atttcgctga cacgatacga agcgagcacg ccggtgaact gcttgggctc gatggcatat
     9001 gcgtacgagc ggctgtagtt cgggttgttg cccgcgtcga agacttcgta gccgatcggg
     9061 ctttcccagt agccgatgcg gaagtccaat ccgttgccca ccggggcccg aagcgtgacg
     9121 gcggcgtcct tgatgccaaa atcactgtcg ccacccagcg aatcggtgcc gtaggtgttc
     9181 gcatccggac cgaagataag cccaacctga tatcccgcgg accattggct ttcgtccagc
     9241 ggtttttcga tctgcagttt gacaacgtcg aggttgaacc cgttgaactt gttgttggcg
     9301 tcaaaggtgc gccccggtgt cgagtagtcg ccgttgggca gactcggccc gaaccgcaga
     9361 atggccgagg tgttcacata accgctgatc gtcgtggacg acaacgcggt caagatggga
     9421 ttctgtgctt cttcggcctg agcggccgta ccgaggctaa ccagacccac cgcggccagc
     9481 ccgagcgtcc attggttaaa cttcattcgc tttcctcctg cgatgtaaca tgtttccgtt
     9541 attgcttcga ttcgttgctt gttgtgttct gcgtcgctga aaatgccagg gtaaacgctc
     9601 tttgtctgcg ttggccccgc cgcattggga acgcagggta gcagaaaccc ccgtgctgac
     9661 aacaagtttc ttttatgctg cggccctagg acgatcccaa atcatcgcct aagcggggtg
     9721 cgtcgaaaat tccgagaact gcccgcccg
//
LOCUS       DAOQRO010000102         9743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6332_length_9743_cov_5.088357, whole genome shotgun sequence.
ACCESSION   DAOQRO010000102 DAOQRO010000000
VERSION     DAOQRO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6332_length_9743_cov_5.088357"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKK20_05485"
     CDS             complement(<1..329)
                     /locus_tag="PKK20_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99369.1"
                     /translation="MLKTRSTPWPMVLRIILCLVLLGWVFQAIFLSEGRSAYERQGLG
                     WDSLSHAEQWKLAWHYGPPELWRNVCLIDPTAAVWSLVFMGATIVLGTLRWRMVLRVH
                     GINLSLGR"
     gene            complement(354..1268)
                     /locus_tag="PKK20_05490"
     CDS             complement(354..1268)
                     /locus_tag="PKK20_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNU99370.1"
                     /translation="MYALSTCWNSSRHIDGHAMLREIRELGFEYAELSHGIRLSLVPG
                     ILEAVAAGVVRITTLHNFCPLPIGITQAAPNIFKFTSSDARERQSALKHTLKTFELAE
                     RLGARLVVLHMGAVEMKDYTDALIRLAEAGLRETPKYQRLCAEADERRESKKERHLHF
                     AYEALRYLESEAARRHLLLGIENREALEEIPFESDFPFFFRDFPGGVIRYWHDTGHAQ
                     IKENLGFIDHVMHLESLAGDIAGFHLHDVEFPGRDHRAPGKGMVNFEALKPFVKLEHI
                     KVLELSPSIPAEEVRAGLAHLQSLWGPE"
     gene            complement(1348..1545)
                     /gene="rpsU"
                     /locus_tag="PKK20_05495"
     CDS             complement(1348..1545)
                     /gene="rpsU"
                     /locus_tag="PKK20_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNU99371.1"
                     /translation="MTEIRLKKGESVEKALRRLKKKVDREGTLKEVRNHRHYEKPSER
                     RRRKLKVAKFSAMLAARYADM"
     gene            1848..2840
                     /locus_tag="PKK20_05500"
     CDS             1848..2840
                     /locus_tag="PKK20_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNU99372.1"
                     /translation="MKRLLILTSLGLLAGATLPAAAQSFPKLTDAQRIALLQPPTQRP
                     VPMVLDTDTYNEIDDQFALVYALISPELKVEAVYAAPFKNDRSTSPENGMERSHEEIV
                     RILDKLNRSPENFALKGSRRYLTDPKKPEESPAALDLIRRAKAHSPDRPLYVVAVGAI
                     SNVANALLLDPAILPNIVVVWLGGNGLGWPHQHEFNYHQDLNASKIIFNSGVALVQLP
                     CTPVVTHFATTVPEMEANLAGRGAIGEYLLEIFKDYHQDHFAWSKVLWDMTAVAWVID
                     SRWLPSNLVHSPIPTDNYTFSFDNSRHLIRSVYFLQRDPIFRDFFTKLQKLARP"
     gene            complement(3079..5955)
                     /locus_tag="PKK20_05505"
     CDS             complement(3079..5955)
                     /locus_tag="PKK20_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99373.1"
                     /translation="MKHSFGIGPLVLVGGLCLTLCVSPGRGAESMTLGNGEITADFDA
                     AGLVRVSLAKSGHALSLAGDSTSITVDGQTLSVRDLERTGLAQRGQSVVYTYAAGERR
                     LEVIYELKPGWQFISKQIALILPPQSACRLDTVEGLRIEAGTPIAREHRASNASGAVF
                     LRLGRSDPAPGLGACLVLQNPFLKWQRQDARISMSYSPDLEWRASYGPFVSDRVCLGF
                     YRLSGVEFPARNLPEWKHVPDPERAFEGLPMLDRAEFEAVTGCVDAFALFRPTKSLRV
                     HVPWCENDYQIDVGTPAGRAEWKRILDQCAAVGIEHTLFTPANSAVAPLADNADAWGW
                     ENCLWLGLGQKIRKGEWDVATDPIPPSIQEMLDYAKAKQVKLVAYAYPTLGWKQNPEW
                     TAWCGGKTGGYIGVDTGVRSFQDWFVGQLVAFQQRTGIGGYSFDHWWIAYEPNKEGHK
                     PTSKYAQWSGCRRILEELRRRLPDIVIDGRQQYQWFGPWTWLGGSYPHPTTNDEQPGS
                     FENFPDLHFSRVSGNRQRWATGYFHQEQFTPWELVPGYMTHQTPRNNAKGECVKNRPF
                     HTRDWDLLGWRYSVLSSIGTAPFNHVVSLLPARDETEFAHFGPDDQRWLRGWMDWTDR
                     HRAILKRLRPIIGLPAMGRVDGTAAIAGDHGFVFLFNPNYRELPAEFTLDAGIGLTEG
                     EEFLLRELYPREGRLRGRPRVGVWRRGDRVRLPIKGPEALVVEVVSADAVTRPALINA
                     RGRATLSGDRLELANVEGQMGHTHELVVLLPARESVSRVVLNGREQAGFSQEGDAVTL
                     RAAFDGERFDHCHQVDTYDPAFAGRVVRADFTVPQRVFAQLAARRKAWPVPYTEEELL
                     ATWRGSDRLLLFIQIADPDDTWAVGLTLDGQPVEVRKAYSDVFPLGRERTFTGLYADL
                     SHLAPDTSHHIEVTLPEGLKPGQFQGLFFENVEAEWTTAIGARP"
     gene            6183..6842
                     /locus_tag="PKK20_05510"
     CDS             6183..6842
                     /locus_tag="PKK20_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNU99374.1"
                     /translation="MNTFDAIIFDMDGVIVDSEPRHERAFREIFDELGYGDRHGMDFA
                     AYYGRSDRAFWLDFVAKHKPRQSLEELLALKQNRLIDILRRDQPLFDGLPDLLERLAP
                     RYALAVASGSNHPVIEEVLAMRQLGRFFSVVVSVQDVPRGKPAPDVFLRAAQMLAVPA
                     ARCCVVEDSAHGVEAARAAGMEVIAITNTLPSERLAAASHVVETYEEIESLLLEPVRD
                     P"
     gene            6839..8185
                     /locus_tag="PKK20_05515"
     CDS             6839..8185
                     /locus_tag="PKK20_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNU99375.1"
                     /translation="MNNAIDEHATPGAAWHRFFDFDAHKTSPRKEAMAGLVTFMTMAY
                     IIFVNPAMLAGAGLSKAAVVTATCLGAAVPTLLMGLWAKYPLALASGMGLNAALVMQA
                     SQPGVTWQTMMGVVVVEGALVTLLVLTGVREQVMQAIPMNLKRAIGIGIGIFIAFLGL
                     QQMGWVAKGPGGAFLAPGNFKVLPTLIASVGLALTLTLLAFRVRGAILWGILGTAALA
                     WLSDRLLPGGPALVAVPEQLMATPEFATFGAADLVGALRPALWGVIFAFLITDFFDTM
                     GTVIAVGTQGGYMDAQGQLPRLKRVLVVDSLAAAWGGFCGASSVTTYIESAAGVSAGG
                     RTGFVSVVVAALFFLAMFFAPVIGAVPGIATAPALVVVGFLMMSLVREMDLESPDEGM
                     PAFLTLLLIPMTQSISCGIGVGFIATVVVKVLGGKARDVSPWFYGIALLFVLHFAL"
     gene            8195..9295
                     /gene="dusB"
                     /locus_tag="PKK20_05520"
     CDS             8195..9295
                     /gene="dusB"
                     /locus_tag="PKK20_05520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNU99376.1"
                     /translation="MRLGALELASNLFLSPLAGYTNLPFRLTIRELGGLGLCTTELVN
                     ARSLIEKRDKALKLIESNARDRPLAVQLFGAVPEEMRDAAQCLESLGADAIDINMGCP
                     VRKVCRVGGGSAMMSELSKTAALAQGIVRVVRVPVTAKMRLGWDDANLTAPDLARALE
                     DVGVSAVFIHGRTREQGFGGCVNLAGIRAVVDAVRRIPVIGNGEVTTPQAAKRMLDET
                     GCAGVSIGRGAFYNPWIFRQTEAFLASGELPSEPPFEERVRVMCRHLDLMIEVFGETH
                     GCRMFRKVAPWHAKRLGPAVEFKRRVASLSSRAQFDDVLAEYRAWRAQFLDDRGELLP
                     RFAPTPSNPSFMTGEPAAPSAIPTPRGPVETW"
     gene            complement(9359..9736)
                     /locus_tag="PKK20_05525"
     CDS             complement(9359..9736)
                     /locus_tag="PKK20_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004044638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU99377.1"
                     /translation="MNSKPNVIVQRADDVVRQAVSTGVGISMQMLIGPDVAPNFLLRR
                     FVIEPGGSMPNHTNQVEHGQYVLRGRAKVGIGDRVYEVQSGDVVFIPQGWPHWYKTEG
                     NEAFEFLCAVPNVPDKTTLLDRD"
BASE COUNT         1733 a         3197 c         3006 g         1807 t
ORIGIN      
        1 cgcccgaggg agaggttgat gccgtgtacg cgcaggacca tccgccaacg gagggttccc
       61 aggacgatcg tggcgcccat gaagaccaga gaccaaaccg cggccgtcgg gtcgatcagg
      121 cagacgtttc gccacagctc cggcgggccg tagtgccagg ccagcttcca ttgctcggcg
      181 tggctcagcg aatcccagcc aaggccctgc cgctcgtacg cactgcgtcc ctcgctgagg
      241 aagatcgcct gaaacaccca gccgagaagg accaggcaaa ggatgatgcg caacaccatt
      301 ggccagggag tcgagcgcgt cttcaacatg cgatggcagg aaggtccgac ctcctactcg
      361 gggccccaaa gcgactgcag gtgggccaga ccggcccgca cctcctcggc cgggatcgag
      421 ggcgagagtt cgagcacctt gatgtgttcg agcttcacga acggtttcaa ggcttcgaaa
      481 ttgaccatgc ctttccccgg cgcgcgatgg tcgcgtccgg ggaactcgac atcatggagg
      541 tggaatccgg cgatgtcgcc ggcgagcgac tcgagatgca tgacgtggtc gatgaacccg
      601 agattctctt tgatctgagc gtggccggtg tcatgccaat accggatcac cccgccggga
      661 aagtcgcgga agaagaaggg gaaatcggac tcgaacggaa tttcttcgag ggcctcgcgg
      721 ttctcgatcc ccaggagcaa atggcgccgc gcagcctcgg actcgagata gcgcagcgcc
      781 tcgtacgcaa agtgcagatg ccgttccttc ttcgactcac gccgctcatc ggcctcggcg
      841 cacagccgct ggtacttggg cgtctctcgc aaacctgctt ccgccagccg gatcagagcg
      901 tccgtgtagt ctttcatctc gacggctccc atgtggagca ccacgagccg cgcgcccagg
      961 cgctccgcca actcgaacgt cttgagggtg tgtttgagcg cgctctgccg ctcccgagca
     1021 tccgacgacg tgaacttgaa gatgttgggg gcagcctgcg tgatcccgat cggcagcgga
     1081 cagaaattgt ggagggtggt gatccgcacc acgcccgccg cgacggcttc gaggatgccc
     1141 ggcaccagac tgagccggat tccgtggctg agttccgcgt actcgaaacc caactcccga
     1201 atctcgcgca gcatggcatg gccgtcgatg tggcggctcg agttccagca ggttgacaac
     1261 gcgtacatag acaacaacaa aggccgagcg cgcgtgcgca cggcacgggc tcggccctga
     1321 aatcagcacc tgttcctgag tccccgccta catgtcggcg tatcgggcgg ccaacatggc
     1381 cgagaacttg gccactttga gcttgcgccg acgccgttcg cttggcttct cgtaatgccg
     1441 atggttgcgc acctctttca gggtgccttc acggtcgact ttcttcttca aacgccgaag
     1501 cgccttctcg accgattcgc cttttttgag ccggatttcc gtcaaaatcg attcacttcc
     1561 tctctgggac ccggggccgc ttggccgcac agcgcggcca gccccctttc aggtcagggc
     1621 ggggacagta ggtggaaccg ctgccggatg tcaatcggga atccgcaggg caccgcacac
     1681 tccggattag ccttgccgcc ctgcaactcg atttccgcat cgattcactg accccgactg
     1741 caagattcgg ccgcgctttc cccgcacccc ccctgacacg cgcgaggtcg tgcgcgtcga
     1801 tttctctgcc atctcgggcg ctcccgaact agactctcgc cccagctatg aaacgccttc
     1861 tgatcctgac cagtctcggc ctcctggccg gagcgactct tccggccgcc gcgcagtcgt
     1921 tccccaaatt gaccgacgcg cagcgcatcg cacttttgca gcctcccacc caacgtcctg
     1981 tcccgatggt gctggacacc gacacctaca acgaaatcga tgatcagttc gccctggtct
     2041 acgcgctcat ctcgccggag ttgaaggtcg aggccgtcta tgccgccccg ttcaagaacg
     2101 accgttccac aagcccggag aacggcatgg agcggagcca cgaggaaatc gttcgcatcc
     2161 tcgacaagtt gaaccgttcc ccggagaact tcgccttgaa aggcagccgc cgctacctca
     2221 ccgaccccaa gaagccggag gaaagtccgg cggcgcttga cctgatccgc cgggcaaagg
     2281 cgcacagtcc ggaccgcccg ctttacgtcg tggccgtggg agcaatcagc aacgtggcca
     2341 acgccctgct cctcgatccc gcgatcctgc caaacatcgt cgtcgtctgg ctaggcggca
     2401 acggtctcgg ctggccgcac cagcacgagt tcaactacca ccaggatctc aacgcctcga
     2461 agatcatctt caattccggg gtcgctctcg tccaactgcc gtgcacgccc gtcgtcacgc
     2521 acttcgccac caccgtgcct gagatggaag ccaatctggc cggccgcggc gccatcggcg
     2581 aatacctgct cgaaatcttc aaggactatc atcaggacca tttcgcgtgg tccaaggtgc
     2641 tctgggacat gaccgcagtg gcgtgggtga tcgacagtcg atggctgcca tcaaacctcg
     2701 tccacagccc gatcccgacc gacaattaca ccttcagctt cgacaactcc aggcacctca
     2761 tccgcagcgt ctacttcctt caacgcgacc cgatcttccg cgacttcttc actaagctgc
     2821 agaagctcgc gaggccttag cctgcatttc tcaccgcttt tggacaacgc gaagccggta
     2881 tacacaggag aaatacaaat gcagactaag ggtctgtgca tgcgcagccg cgggatcggc
     2941 cctccgaaac gaaaggggac ggttcatggc aagcgctgtg attaaaccgc ttggtgcgca
     3001 tgaggatcct gaaccgaaaa cgcacaacgc acccactctc gactctcgac tctcgactct
     3061 ccactctctg acacttcgtc agggccgggc gccaatcgcg gtggtccatt ccgcctccac
     3121 gttttcgaag aataatccct ggaactggcc gggcttgagg ccctcgggca gcgtgacctc
     3181 gatgtgatgg ctcgtgtcgg gcgccagatg tgagaggtcc gcatagaggc cggtgaacgt
     3241 gcgctcgcgt cccagtggga acacatcgct gtatgccttc cgcacctcga ccggttggcc
     3301 gtcgagcgtt aatcccacgg cccaggtgtc gtccggatcg gcaatctgga taaacagcag
     3361 caggcgatcc gagccgcgcc aggttgccag caattcttcc tccgtgtaag gaaccggcca
     3421 ggccttgcgg cgcgcggcca gttgcgcaaa gacccgctgc ggaacggtga agtcagcccg
     3481 gacgacccgg cccgcgaacg cgggatcgta ggtgtctact tggtggcaat ggtcgaagcg
     3541 ctcgccgtca aatgcggcgc gcaaggtcac cgcatcgcct tcctggctga aaccggcctg
     3601 ttcacgcccg ttcaaaacga cccgggacac gctctctcga gccgggagca ggacgaccaa
     3661 ttcatgtgtg tggcccattt ggccttcgac attcgccaac tcgagacgat cgccgctgag
     3721 agtggcccgc ccgcgcgcgt tgatcagcgc cggacgcgta acggcgtccg ccgacacgac
     3781 ctcgacgaca agcgcctcgg ggcccttgat cggcaagcgg acgcgatcgc cccggcgcca
     3841 tacgccgacg cgcggtctgc ctcggaggcg tccctcgcgc gggtagagct cgcgcaacag
     3901 gaattcctcg ccctccgtaa gcccgatgcc ggcatccagg gtgaactcgg ccggcaactc
     3961 ccgatagttt ggattgaaga ggaacacgaa tccgtgatcg ccggcgatcg ccgcggtgcc
     4021 gtcgactctt cccatggccg gcaggccgat gatgggccgc agccgtttca ggattgcccg
     4081 atgccggtcg gtccagtcca tccaaccccg gagccagcgc tggtcgtccg ggccaaagtg
     4141 cgcgaattcg gtctcatcgc gcgcgggcaa cagactgacg acgtggttga agggggcggt
     4201 gccgatcgag gaaaggacgg aatagcgcca gccgagcagg tcccagtcgc gcgtgtggaa
     4261 ggggcggttt ttgacgcatt cgcctttggc attattgcgg ggcgtctggt gggtcatgta
     4321 accgggcaca agttcccagg gcgtgaactg ctcctggtgg aagtaccccg tggcccagcg
     4381 ctgccggttg ccggacacgc ggctgaaatg gaggtcgggg aaattctcga agctgccggg
     4441 ctgctcgtcg tttgtggtcg gatgcggata ggatccgccc agccaggtcc agggaccgaa
     4501 ccactggtat tgctggcgcc cgtcaatgac gatgtccggg aggcggcgtc gcagttcctc
     4561 gaggatgcgt cggcagccgg accattgggc gtacttgctg gtcggcttgt gcccctcctt
     4621 gtttggttcg taggcaatcc accagtggtc aaagctgtag ccgccgatcc cggtgcgctg
     4681 ctggaaggca accagttgcc cgacaaacca gtcctggaaa ctccgcacgc ctgtatcgac
     4741 tccgatgtaa ccgccggtct tgccgccgca ccaggccgtc cactcggggt tctgcttcca
     4801 gcccagggtc ggataggcat aggcgacgag cttgacctgt ttcgccttcg cgtaatcgag
     4861 catttcctgg atcgagggcg ggatcgggtc cgtcgcgacg tcccattcgc ctttgcggat
     4921 cttctggccc aggccgagcc agaggcagtt ctcccaaccc caggcgtcgg cgttgtccgc
     4981 gagcggggcg acggcgctat tggccggcgt gaagagcgtg tgctcgatgc ccaccgccgc
     5041 acactggtcg agaatgcgct tccactcggc gcgaccggcg ggcgtgccca cgtcaatctg
     5101 atagtcgttc tcgcaccagg ggacatggac acgcaggctc ttcgtggggc gaaacagggc
     5161 gaaggcgtcc acgcaaccgg tcacggcctc gaactcggcc cgatcgagca tcggcaggcc
     5221 ctcgaaggcg cgctcggggt cgggcacatg cttccattcg ggcagattcc gcgccggaaa
     5281 ttcgactccc gagagccggt agaacccgag acagacgcgg tccgagacga atggtccgta
     5341 tgaggcgcgc cattcgaggt cgggcgagta gctcatcgag atccgggcgt cctgccgctg
     5401 ccacttgaga aacggattct gcagcaccag gcaggcgccc aagccgggtg ccggatcgct
     5461 tcttccgaga cgcaggaaca ccgcgccgct ggcattgctg gcccggtgct cgcgggcgat
     5521 cggcgtgccg gcctcgattc ggagcccttc gaccgtgtcg aggcggcagg cgctctgggg
     5581 cggcagtatg agcgcgatct gcttgcttat gaactgccag ccgggcttca gctcgtagat
     5641 tacttcgagt cgccgctcgc ctgccgcgta cgtgtacacg acactctgcc cgcgttgcgc
     5701 gaggccggtc cgttcgaggt cgcgcacgct gagcgtctgg ccgtctacgg tgatcgaggt
     5761 cgagtcgccc gcgagcgaga gggcgtgccc ggatttggcc agcgacaccc ggaccaggcc
     5821 cgcggcatcg aagtcggccg tgatctcccc gttgccgaga gtcatgctct ccgcgccgcg
     5881 cccgggcgac acgcacagag tcaggcaaag cccgccgacc aggacaagtg gaccgattcc
     5941 aaacgagtgc ttcatgggtc catcgtgacc gggtctggaa aattctcaag gctatcggca
     6001 gcctgattgg cggaagacgt tacggaacgg cggttcatga aatggagctg acaagccggg
     6061 atttgactgc atcggggctg cgacaatact ctcgcgccgc actgctggaa tgtgcctccg
     6121 gcccgaagcc agggggcgca tgatcgcagg agaatccggt ccgaacattc ttgaaggcct
     6181 cagtgaacac ttttgacgcc atcatattcg acatggacgg tgtgatcgtc gacagcgagc
     6241 cccgccacga gcgggcgttt cgggagattt tcgacgagtt gggctacggc gaccgtcacg
     6301 gaatggattt cgcggcgtat tatggccgat cggatcgcgc gttttggctc gattttgtcg
     6361 ccaaacacaa gccacgccag tcgctcgagg agcttctcgc cttgaagcag aaccggctca
     6421 ttgacattct ccgacgcgac caaccgctct tcgatggcct gcccgatttg ctcgaaaggc
     6481 tggcgccgcg ctacgcgctg gcagtcgcct cgggatcgaa tcacccggtg atcgaagagg
     6541 tcctggccat gagacaactc gggcgtttct tctcggtcgt ggtcagcgtg caggatgtcc
     6601 cgcgcggcaa gcccgccccg gacgtcttcc tgcgcgcggc gcaaatgctg gccgtgccgg
     6661 ccgcccggtg ctgcgttgtc gaggactccg cccacggggt cgaggccgcg cgcgcggccg
     6721 ggatggaagt cattgccatc accaacaccc tgccgtccga acgcctggcg gctgcaagcc
     6781 acgtcgtcga aacctacgaa gagatcgagt cgcttctgct cgaaccggtc cgtgacccat
     6841 gaacaacgcc attgacgaac acgccacccc aggcgcagcc tggcatcggt tcttcgactt
     6901 cgacgcccac aagacctcgc cacgcaagga ggcgatggcg ggcctggtca ccttcatgac
     6961 catggcctac atcatcttcg tcaatccggc catgctcgcc ggcgcaggct tgtccaaggc
     7021 cgcagtcgtg accgcaacct gcctcggcgc cgcggtgccg acgctgctga tgggtttgtg
     7081 ggccaagtac ccgctggcgt tggcgagcgg catgggtttg aacgccgccc tggtcatgca
     7141 ggcttcccag cccggtgtga cctggcagac catgatgggc gtcgtcgtcg tcgaaggcgc
     7201 cctggtcacc ctcctggttc tgacgggcgt gcgcgagcag gtgatgcaag ccataccgat
     7261 gaatctcaag cgggcgatcg gcatcggcat cggcatcttc atcgccttcc tcggactcca
     7321 gcaaatgggc tgggtcgcca aaggccctgg cggcgccttc ctggcgccgg gtaacttcaa
     7381 agtgctcccg actctgatcg ccagcgtcgg cctcgctctc accttgaccc tgctcgcgtt
     7441 ccgagtccgc ggcgccattc tctggggcat cctcggcacg gcggcgcttg cctggctctc
     7501 ggatcggctc ctcccaggcg ggcctgcgct cgtggcagtg cccgaacagt tgatggcgac
     7561 acccgagttc gccacgttcg gcgcggccga tcttgtggga gctctcagac cggccctgtg
     7621 gggtgtgatc ttcgcctttc tcatcaccga tttcttcgac accatgggca cggtgatcgc
     7681 cgttgggacg cagggtggct acatggatgc ccagggccaa ctgccccgcc tcaaacgggt
     7741 cctggtggtg gattcgctcg ccgcggcctg ggggggcttc tgcggagcca gttccgtcac
     7801 cacctatatc gaaagcgcgg ccggggtgag cgccggcggg cggacgggtt tcgtgtcagt
     7861 ggtcgtagcc gcgctctttt tcctggccat gttcttcgcc cccgtcattg gggcggttcc
     7921 agggatcgca accgcacccg ccctggtcgt ggtcggtttt ctcatgatga gtttggtgcg
     7981 cgaaatggac ctagaaagcc ccgacgaagg gatgccggct tttctgacct tgctgctgat
     8041 cccgatgacg cagagcatct cctgcgggat cggggtcggg ttcatcgcca ccgtggtggt
     8101 caaggtgctc ggcggcaaag cgcgcgacgt gtctccctgg ttctacggga ttgccctcct
     8161 cttcgtcctc cattttgcgc tctagaaact tatgttgcgg ctcggcgcac tcgagctggc
     8221 ctcgaatcta ttcctgtcgc ccctggccgg atacaccaac ctgccctttc gcctgacgat
     8281 ccgggaattg ggcggcctcg gtctctgcac aaccgaactg gtcaacgccc gttccctgat
     8341 cgagaaacgg gataaggccc tcaagctcat cgagtccaac gcacgcgacc gccccctggc
     8401 cgtgcaactc ttcggcgccg tgcccgagga aatgcgcgat gccgcccaat gcctcgaatc
     8461 gctgggcgcc gacgcgatcg acatcaacat gggatgcccc gtgcgcaagg tctgccgcgt
     8521 cggaggcggg tccgccatga tgtccgaatt gtcgaagacc gccgcgctcg cgcaggggat
     8581 cgtccgcgtc gtgcgcgtcc cggtcaccgc aaaaatgcgg ttgggctggg atgacgcgaa
     8641 tctcacggcg cccgacttgg cccgcgccct cgaagatgtc ggcgtgtccg ccgttttcat
     8701 ccatggccgc acgcgcgaac aaggctttgg aggatgtgtc aacctcgcgg gcatccgggc
     8761 ggttgtcgat gccgttcgca gaatcccggt catcgggaac ggcgaggtga ccacgccgca
     8821 ggccgccaaa cgcatgctcg atgaaacggg ctgtgccggc gtcagcatcg gacggggcgc
     8881 attctacaat ccctggatat tccgccagac tgaggccttc ctcgcgtcag gtgagctgcc
     8941 gtccgaaccg ccgttcgagg agcgtgtccg cgtcatgtgc cgacacctcg acctgatgat
     9001 cgaggtcttc ggcgaaacgc acgggtgcag gatgtttcgc aaggtcgcgc cgtggcacgc
     9061 gaaacggctc ggaccagccg tcgagttcaa gagacgcgtg gcatccctgt cgagccgcgc
     9121 tcagttcgac gacgtccttg ccgagtatcg ggcctggcgc gcgcagttcc tcgacgaccg
     9181 cggtgaactc ctgccccgct ttgccccgac gccctcgaac ccatcgttca tgaccggcga
     9241 acctgcggcg ccatccgcca tcccgacccc ccgaggcccg gtcgaaacgt ggtgacagaa
     9301 ctcctttcaa aggagtagat gggcgggtga tcgagagttg gcaaggcgat ggattcagtc
     9361 aatcccggtc cagcaaggtc gtcttgtcgg gcacgttggg caccgcacag aggaactcga
     9421 aggcctcgtt tccctcggtc ttgtaccaat gcggccaacc ctgtgggatg aacaccacat
     9481 cgccgctctg gacttcatag accctgtcgc caatgcccac cttcgcacgg ccgcgcaaga
     9541 catattgacc gtgctcgact tggttcgtgt ggttgggcat actgccaccc ggttcgatga
     9601 cgaatcgtcg gaggagaaag ttcggtgcga cgtcgggtcc gattaacatc tgcatcgaga
     9661 tgccaacccc ggtcgacaca gcctggcgca ccacatcatc cgctcgctga acaatcacat
     9721 tcggtttgct gttcatgtca cgg
//
LOCUS       DAOQRO010000103         9703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6385_length_9703_cov_5.348155, whole genome shotgun sequence.
ACCESSION   DAOQRO010000103 DAOQRO010000000
VERSION     DAOQRO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6385_length_9703_cov_5.348155"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4645)
                     /locus_tag="PKK20_05530"
     CDS             complement(<1..4645)
                     /locus_tag="PKK20_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNU99378.1"
                     /translation="MAVLGMLVAVLLGAQDVAGADAFPKPGDANGDGRVDEADAHLIL
                     QKAASAIVLGGVEADAADVNRDGSVDPRDAILALQSAAAGSAFSWEGFEAWAEVRDGQ
                     VAIQLVFGGPSARIGDRVEARLLDLAGGVRQRTTVSLGGSADRRLGAFSFSLAGTASA
                     ADLASYVLDLEGEHGEDTVRERVSLYQLVSRLRMHMFGPERWYASALNRARIAVQDSR
                     DGRPLEGSVVTVALTKPGGGEVYAQVDGRTDADGVVDAALRVPGGLTGDGELVVSVDS
                     PLGRRVMVQSVEIVEGTTTLLTTDKPLYQPGQTIHMRALSLKKPQMVPAAGEEIVLTV
                     ADAKGNKVFKQAGVLNDFGVASADFALATELNLGPYTVAAEIRGGKTEKKVTVERYAL
                     PKFKVALHTDRNFYAPGAKLSGTVAADYFFGKSVQGAAVTIKASKFDVGFEPFAEVSG
                     QTDAQGRFEFSLDLPSHFVGQPLQQGEAFALVEVTVIDTASHQESISRSVPVSASEVV
                     IHAVPESGHLISGVPNRIYILTLYPNNSPAQTTCTVRVGGEVVDTVTTDAAGIGIVTV
                     TPAGQSALTLDLHAADSRGNSADRSVDLAFGGGAEGVLIRTDRALYAVGDVVKADIYC
                     SGLGGSVFVDVVKEGQTLYSRSLSPEGGHTALVLPLTADMSGTLSLSAYRITVGGDTI
                     RDRRIIYVDPANDLRLDYAPDKDVYLPGEEAAIRLQVSGQDGQPVVAAIGLTIVDEAV
                     FALQEMQPGMEKVYFYLEEQLRQPRYEIHGFTPEEIVYDPLGPDSYTARERALGMLFA
                     ALGTVPLGTFERVSDAADGEALRETLRDAVWADLDVLIEMLDEELGWHDHDLSTAYFE
                     IVLRELAEKGHPESCDAVVDPWGTPYRIRYAAATGEYSFLCAGLDKRWDTSDDLTLVA
                     SYWTVRHPWWRENPEWWLIAHGGPQGLVLFAQSFGWLEDGVGDWGPPSVSPSAGGGET
                     QPYLRQYFPETLYANPLVITGADGAAIVNLKVADSITNWRMTGMASSADGRLGSATGA
                     LRVFQEFFVDLDLPPTLTRGDEVSVPIAIYNYLLEGQAIRLAIDAGDGFQLLDAPERT
                     VVLGADEVSVTYFRIKTVRVGRHKLEVTAYGSTRSDAVAREVEVMPDGAEVLVTHSGR
                     LSGTIDRTIPIPAEAIDGASKILVKVYPGLFSQVVEGLDSMLHMPYGCFEQTSSVTYP
                     NVLVIEYMKSTGQITPEILMKAEGFIGAGYQRLLSYEVEGGGFSWFGYQPAHNVLTTY
                     GLMEFSDMSKVWYVDPTVIRRTQDWLVAGQEPDGSWIPTDGGIAEGAIDRFQNDVLRT
                     SAYVVWGLTSSGYSGAAVASGTAYVKAQLARPDWQPETYTLALCAHALLCDANDPLLP
                     GLFEEFERRKHEENDRVWWEETAATMTGSMGQGASVELTGLLAQAYLRFGGYPNTAAK
                     ALNHLASSKDGHGNFGSTQATVQALKAFALAAGGVTTPADATVGIAINGAEVKTLRLT
                     EANSDVLFLCDLGEHTVEGDNAVRLTFEGKGSNLYQLIGRYYLPWEAVDPPGEE"
     gene            4875..5561
                     /locus_tag="PKK20_05535"
     CDS             4875..5561
                     /locus_tag="PKK20_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNU99379.1"
                     /translation="MSGHKVSFLKNEGYIHADPAEAVEYAREPSSLPRNVLTPKEANR
                     IIDSIDTTTALGCRDRTILEVFYAIGIRRTELRNLIIADVNLEEELLRVNSGKGGHDR
                     VVPLSRVACKFLETYIKGVRPQLVAGKTTDKLFVSIRGNPIDVHTVGDVARKYAKLAN
                     MKKHVTPHVWRHTCATHLVKNDANLRHVQDILGHRSLNTTERYLRLAITDLKEAHRNF
                     HPREPRSDKP"
     gene            5603..5848
                     /locus_tag="PKK20_05540"
     CDS             5603..5848
                     /locus_tag="PKK20_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNU99380.1"
                     /translation="MSIAAEKVAEVLALPEQDRAFLARQLIASLDDTVDADAETQWHE
                     VIDRRSREIEEGKVGCRPVEQVVQDIRHKLHARRQPS"
     gene            5826..6125
                     /locus_tag="PKK20_05545"
     CDS             5826..6125
                     /locus_tag="PKK20_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNU99381.1"
                     /translation="MRVVSHPEADEELEAAALWYEERQPGLGDAFLDQFERTLRRIVA
                     EPERWRKIRGDNRKLNFHRFPYAIVYSVRADTIYVKAVMHLHRRPFYWSRRQPTV"
     gene            6219..7094
                     /locus_tag="PKK20_05550"
     CDS             6219..7094
                     /locus_tag="PKK20_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNU99382.1"
                     /translation="MVAAGEHLSSSRPARRAKTRPAARIPLCVEPSTPFAEGPGPGRD
                     WWEKALSEKWFSGPKRGNKQQNQGVGAKSRTTHLGPFGELLRATGPMAKANPYRFSTK
                     YQDDETDLIYYGYRYYNPNTGRWPNRDPLGEKGFETALRTKRCFLCKTAELLEGPNLY
                     AFVANDPLDYVDTDGRGKYKLPPPGPYKPPIPIEPPAPKPSWPKRLWKCAIAPVNAVT
                     CTSCAGGLLGIEAICGGAAGGGWEGMGYSDFNSCVCDFYNDNWLLKQTCNSCFSLPIA
                     GGIGTLFEKVVGWGN"
     gene            7101..7508
                     /locus_tag="PKK20_05555"
     CDS             7101..7508
                     /locus_tag="PKK20_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99383.1"
                     /translation="MRAASDSMRRFGDWLQSSKPEIKKRGKHLPVIGLLCLTAGCIAY
                     YAMTGQLKNLCSLETLRPALSVGVLFGLCSYIIIGKAGLVFLKMLLCLGLIAWIIGWL
                     TWEAPSPITVIGQLTGWGLMFAWVWSLEPTETQ"
     gene            complement(8244..9416)
                     /locus_tag="PKK20_05560"
     CDS             complement(8244..9416)
                     /locus_tag="PKK20_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNU99384.1"
                     /translation="MKDSKRDRPQPDWDPTSPEVQRDQRAAYDDMRRRCPVAYSEFMG
                     WSLFRHEDVMRVLLDHKTFSNAVSQHLSVPNGMDPPQHTAYRRVIESYFSARHLELFA
                     PICRDIVIRLVTELITQAETEFIAAFALPFAVRVQCAFLGWPETLHKPLVRWTRKNHE
                     ATLAQDRKAMSEIALEFEGIIDDLLEARMDAAAAPETDLTAALMHEKVWGRVLSNEEI
                     ASILRNWTVGEIGTISASAGILTHYLATHPELQNRLRAGPDLAPAAIEEILRLHGPLV
                     SNRRIAKRAVEIGGRRINAGDRITLMWIAANRDERAFEEPDACRLDRDQSKNLLWGAG
                     IHICPGAPLARLELRLFLEELLSRTSEISLSSTKKTKFAVFPASGFSVLPVRVKSA"
BASE COUNT         1898 a         3148 c         2867 g         1790 t
ORIGIN      
        1 gttcttcgcc cggcgggtcc actgcctccc acggaaggta gtagcgcccg atcaactgat
       61 agagattgct gcccttgccc tcgaacgtga ggcgcaccgc attgtcgcct tcgacagtgt
      121 gctcgcccag gtcgcagaga aagagcacat cgctgttggc ctcggtaagg cgcagcgttt
      181 tgacctcggc gccgttgatc gcgatcccga cggtcgcgtc tgcaggtgtc gtgactccgc
      241 cggcagcgag cgcaaaagcc ttgagcgcct ggacagtcgc ctgggtggat ccgaagttgc
      301 cgtgtccgtc tttcgatgat gcaaggtggt tgagcgcctt ggcggcggta ttgggataac
      361 cgccgaatcg aaggtaggcc tgcgcaagca ggcccgtcaa ctcgacggac gccccctgcc
      421 ccatcgagcc ggtcatcgtt gcggcggttt cttcccacca gacgcggtcg ttctcttcgt
      481 gcttgcgacg ctcgaattcc tcgaacagcc ccggcagcag cggatcgttg gcgtcgcaca
      541 agagcgcatg ggcgcagagc gccagcgtat aggtttcagg ctgccaatcc gggcgggcga
      601 gctgggcctt gacgtaggcg gtgccggaag ccaccgccgc cccactgtaa ccggaactgg
      661 tcagccccca gacgacatag gcgctcgtgc gcagaacgtc gttctggaac cggtcgatcg
      721 cgccctcggc gatccccccg tcggtcggaa tccaggagcc gtcaggttcc tgtccggcca
      781 ccagccagtc ctgggtgcgc cggatcacgg tcggatccac gtaccaaacc ttgctcatgt
      841 cgctgaactc catcagccca taggtggtca acacgttgtg cgccggctga tagccgaacc
      901 acgaaaaacc gccaccctcg acttcgtagc tgagcaaccg ctggtaaccc gcgccgatga
      961 acccctcggc cttcatgaga atctccggtg tgatctgacc cgtgctcttc atgtactcga
     1021 tcaccagcac gttgggatag gtgactgacg aggtctgctc gaagcacccg tatggcatgt
     1081 gcagcatcga gtccaatccc tcgaccacct ggctgaaaag acccgggtag accttcacga
     1141 gaatcttgct ggcgccgtcg atcgcctctg ccgggatcgg aatcgtgcga tcgatcgtgc
     1201 cgctcagacg cccgctgtgg gtcaccaaga cctcggcgcc gtccggcatc acctcgacct
     1261 cacgcgcgac ggcatcgctg cgcgtgctgc cataagcagt cacttcgagc ttgtgccggc
     1321 cgacccgcac ggtcttgatc cggaaatacg tcacgctcac ttcgtccgct ccaagcacaa
     1381 ccgtccgttc cggcgcatcc agcaactgga aaccatcccc ggcgtcgatc gccagccgga
     1441 tcgcctgccc ctcgagcaga taattgtaga tcgcaatcgg cacggacacc tcgtcgccgc
     1501 gcgtcagcgt gggcggcaaa tcgagatcca cgaagaactc ctggaaaacc cgcaacgcgc
     1561 cggtggcgct gccgagccgt ccgtcggcgc tcgatgccat cccggtcatc cgccagttcg
     1621 tgatcgaatc ggccaccttg aggttcacga tggcggcgcc gtcggccccg gtgatgacca
     1681 acggattggc atagagcgtc tcaggaaagt actgccgcag gtagggctga gtctcaccgc
     1741 cgccggcgga gggactgacg gagggcggtc cccagtcgcc gacgccatct tcgagccaac
     1801 cgaaactctg ggcaaatagc acgagacctt ggggtccgcc atgagcgatc agccaccact
     1861 cggggttctc ccgccaccag gggtgccgga ccgtccaata gctagccacc agggtgaggt
     1921 cgtcggatgt gtcccagcgt ttgtcgaggc cggcgcagag gaaactgtac tcgcccgtcg
     1981 ccgccgcata ccggatgcgg tatggcgttc cccagggatc caccaccgcg tcgcaggact
     2041 ccggatgccc tttttctgcc aactcccgga gcacaatctc gaagtaggcc gtcgagagat
     2101 cgtggtcatg ccaacccagt tcctcatcga gcatctcgat caacacgtcc aaatccgccc
     2161 aaactgcgtc gcgcagggtc tcgcgcagcg cctcgccatc cgccgcatcg ctcacccgtt
     2221 cgaaggtgcc aagcgggact gtccccagcg cggcgaacag cattcccaag gcccgctcgc
     2281 gcgccgtgta cgaatcgggc ccgagagggt cgtagacgat ctcctcgggc gtgaagccgt
     2341 ggatctcgta acggggctgg cgcaattgct cttcgaggta gaagtagacc ttctccatgc
     2401 ccggctgcat ttcctgaagg gcgaaaacgg cttcatcgac tatggtgagt ccgatggccg
     2461 ccactacggg ctgaccgtcc tgaccgctga cctgcagccg aatcgccgcc tcctctccgg
     2521 gcaggtagac gtccttgtcg ggagcgtagt cgaggcggag atcgttggct gggtccacat
     2581 agatgatccg ccggtcccgg atcgtgtcgc cccccaccgt gatgcgatag gccgagagcg
     2641 agagggtgcc cgacatgtcc gccgtcagcg gcagcaccag cgcggtatgc ccgccctcgg
     2701 gcgacaggct ccggctgtag agggtctgcc cctccttcac cacatcgacg aacaccgatc
     2761 cgcccaggcc gctgcaatag atgtctgcct tgaccacgtc gcccaccgcg tacagagcgc
     2821 ggtccgtgcg aatcagcacg ccttccgcgc cgcctccaaa agccaggtcc accgagcgat
     2881 cggcgctgtt cccccgcgag tcggctgcat gcagatcgag agtgagcgcg gactgcccgg
     2941 ccggcgtgac ggtgacgatc ccgatgcccg cggcatcggt cgtcacggtg tcgaccacct
     3001 ccccgccgac ccgcaccgtg cacgtcgttt gcgccgggga attgttcgga tacagcgtca
     3061 gaatgtagat gcggttcggg acgccggaaa tcaggtgtcc gctctcggga accgcatgaa
     3121 tcacaacctc gctcgccgac actgggacgc tgcgcgaaat cgactcctgg tgcgaggcgg
     3181 tgtcgatgac cgtcacttcg accaacgcga acgcctcgcc ctgctgcaaa ggctggccga
     3241 caaaatgcga gggaagatcg aggctgaact cgaaccggcc ctgggcatcg gtctgccccg
     3301 agacctccgc aaagggctcg aacccgacat cgaatttcga cgccttgatc gtcaccgcgg
     3361 cgccctggac gctcttgccg aagaagtaat cggcggccac cgtgccgctc agcttcgcgc
     3421 cgggcgcata gaaattccgg tcggtgtgca gcgcgacctt gaacttcggc agcgcatagc
     3481 gctcgacagt gactttcttc tccgtcttgc cgccgcgaat ctccgccgcc actgtgtacg
     3541 ggccaaggtt caactccgtg gccagcgcga aatcggccga tgcaaccccg aaatcgttga
     3601 gaacgccggc ctgcttgaaa actttgttcc cctttgcgtc cgcgacagtc aggacaatct
     3661 cctcgcccgc cgcgggcacc atctgaggct tcttgaggga caatgcgcgc atgtgaatgg
     3721 tctggccggg ctgatacagc ggtttgtccg tcgtcagaag cgtcgtcgtc ccctcgacaa
     3781 tctcgacgct ctggaccatc acccgacgcc ccaaaggcga atcgacactc acaaccaatt
     3841 ccccgtcccc agttagcccc cccggcacgc gcagcgcggc gtccaccacc ccgtcggcat
     3901 ccgtcctccc atcgacctgc gcgtacacct cgcctccgcc cggcttggtc aacgccaccg
     3961 tcaccacgct cccctcgagg gggcgcccgt cgcggctgtc ctggactgca attctggcgc
     4021 ggttgagtgc ggatgcgtac caccgttcag ggccgaacat gtgcatccgc aatcgggaga
     4081 ccagttggta gaggctgact cgttcacgaa cggtgtcctc tccgtgttct ccttcgagat
     4141 cgaggacata agacgccaga tcggctgccg aggcagtgcc ggccagtgaa aagctgaacg
     4201 cccccaacct ccgatccgcg cttccaccca ggctgaccgt tgtcctttgc cgaacgccac
     4261 ccgccagatc gagcaagcgg gcctcgaccc tgtcgccgat ccgcgccgac ggccctccga
     4321 acaccaactg gatggcgacc tggccgtcgc gcacctccgc ccaagcttcg aatccttccc
     4381 acgagaacgc gctccccgcc gcggcggatt gcagcgcaag gattgcgtcc cgtggatcca
     4441 cgctgccatc gcggttgaca tccgccgcgt cggcctcaac acccccaaga acaattgctg
     4501 aagccgcctt ctgtagaatc aggtgcgcat ccgcttcatc gacccgaccg tctccgttgg
     4561 catcgcccgg tttgggaaaa gcgtccgccc ccgcgacgtc ctgcgcaccg aggagaacgg
     4621 cgaccagcat ccccagaaca gccatcgccg gaaccatcgg ccccggtcgg tcatgggcat
     4681 gcgtccgggc aacattcctg cacctggaag aagcccgctt ccgaagcagc cgaagagatt
     4741 tcatggaaac acttggttaa cgccgcgaac atacaccgcg cgcccccgat tgcaaccggc
     4801 aaacgaactc ggtcagagtg ctacccgcaa cgttcgcgcg aatccgcctg tctctgtcgg
     4861 cggccgccac gggggtgagc ggccacaagg tcagcttcct caaaaacgaa ggctacatcc
     4921 atgccgatcc cgccgaggct gtcgagtacg cgcgtgagcc aagcagcctg ccccggaacg
     4981 ttctgacacc gaaggaagcc aatcgcatca ttgactcgat tgacaccacg acggcgctcg
     5041 gctgccgcga ccgcaccatc ctggaagtgt tttacgcgat cggcatccgg cgtaccgagt
     5101 tgcgcaacct catcattgcc gacgtgaacc tggaggaaga actgttgcgc gtgaacagcg
     5161 gcaagggcgg acacgaccgc gtggtgccgt tgagccgcgt tgcctgcaag tttttggaga
     5221 cctacatcaa aggcgtgcgc ccgcaactcg tcgccggaaa gactactgac aaactctttg
     5281 tgtccatccg gggcaatccg atcgacgtgc acaccgtggg tgacgtggcc aggaaatacg
     5341 cgaagctcgc caacatgaaa aagcacgtca cgccgcacgt ctggcggcac acctgcgcca
     5401 cgcacctggt caagaacgac gccaatctgc gccacgtgca ggacatcctg ggccatcgct
     5461 cgctcaacac caccgagcgt tatctgcgcc tcgccatcac cgacctgaaa gaagcacacc
     5521 gcaacttcca cccgagagaa ccgcgaagcg acaagccatg aacacttgcc agaagacccg
     5581 taaacgccga tattttggcc gcatgagcat tgctgccgag aaagtggccg aggtgctggc
     5641 cctgcccgaa caggaccggg cgttccttgc ccgccaactc atcgccagcc ttgacgacac
     5701 cgtggacgct gacgccgaaa cgcagtggca cgaagtcatt gaccggcgct cccgcgaaat
     5761 cgaggaaggc aaagtcggtt gccgtccggt ggagcaggtt gtccaggaca tccgccacaa
     5821 gctccatgcg cgtcgtcagc catcctgaag ccgacgagga actggaagcc gcggcgctgt
     5881 ggtacgagga acggcagccg ggcctgggcg acgctttcct ggaccagttt gaacgcacgc
     5941 tgcgccgcat cgtggccgag ccggaacgct ggcgcaaaat ccggggcgac aatcgcaagc
     6001 tcaacttcca ccgctttccc tacgccatcg tttacagcgt gcgcgccgac acgatttacg
     6061 tcaaagccgt gatgcacctg catcgccgac cgttttactg gtcgcgccgc cagcccacgg
     6121 tttgacttcc agccgccaaa atccgcttgg cgtcggtggc tgtgcgccag cgctttcgct
     6181 ctggtcacag gcccggcaaa atggcagaat ccgccccgat ggttgccgct ggagaacatc
     6241 tttcctctag ccgtcctgcc cgccgtgcga agacacggcc cgcagcgcga atccccctgt
     6301 gtgtcgaacc gtccacgccc tttgccgagg ggccaggacc ggggcgggac tggtgggaaa
     6361 aggcgctttc cgaaaaatgg ttctccgggc caaaacgcgg aaacaagcag caaaatcagg
     6421 gtgttggtgc aaagtcgcgg actacccact taggcccgtt cggggaactc ctccgcgcca
     6481 ccggcccgat ggccaaggcc aatccctacc gattctcgac gaaataccag gacgacgaaa
     6541 ccgacctgat ttactacggt tatcggtatt acaacccaaa tacaggcaga tggccaaaca
     6601 gagacccgtt gggcgaaaag gggtttgaga ctgcgttgcg caccaagcgt tgcttccttt
     6661 gtaagacagc cgaacttctt gaagggccaa acctctatgc gttcgtggca aacgatcccc
     6721 tcgattacgt ggacactgac ggtcgaggaa aatataagct gcctcctcct ggcccgtata
     6781 aaccgccgat tccgatagaa ccgcctgcac cgaaaccgag ttggccgaaa cggttgtgga
     6841 agtgcgccat cgcaccggtg aacgccgtca catgcacgtc ttgcgcggga ggcctgcttg
     6901 gaatcgaagc gatttgcggc ggcgcagcag gtggtggatg ggagggaatg ggatactctg
     6961 acttcaactc ttgcgtgtgc gacttctaca atgacaactg gctgctcaaa caaacgtgca
     7021 actcctgctt cagtttgccc atagcggggg gaatcggaac tttgtttgag aaggtcgttg
     7081 gctggggaaa ctgacggctt atgcgcgcgg cttccgactc catgaggcgt ttcggcgatt
     7141 ggctgcaaag ctccaaaccc gaaatcaaga agcgtgggaa acacctgccg gtgatcgggc
     7201 ttctgtgcct cacggcgggc tgtatcgcct attacgccat gacagggcag ttgaagaatc
     7261 tgtgttcgct ggaaacattg cgacctgcct tgtcggttgg ggtcctgttc ggcttgtgca
     7321 gctacatcat cattggcaaa gccgggctcg tgtttttgaa gatgttgctc tgtctcggtt
     7381 taatcgcgtg gattatcggt tggcttacgt gggaagcgcc ttcaccgatt accgtgattg
     7441 gtcaacttac cggatgggga ttgatgtttg catgggtttg gtctttagaa ccaactgaga
     7501 cccaatagca gccgggcaat gctcacgcac acgaataagc cccaactccg gctgtcccgc
     7561 acaaccccga ccgctcgccg ccccgcgccg tcatgccggc cgcgagaagc gaaggccagc
     7621 cgtcctgcca tcgcgggccg gctcgctaaa gacaacatca gtctgcccgt ggcgtcgtcc
     7681 gtcccgtcac gcagccggga cgaattgctt tttaattgga gtgtcccgcc cgcgcgccgt
     7741 gccggccgtc gtcaccggca gaacagaaca cgaacgctgc gctgtgctgg aaagtttgtt
     7801 cgctgcgctc aaccgtgaaa acgaatgcgc gcaagttggg ctttgctccg tgggaagccg
     7861 gaggcgcgcc ggtcactcgt cgtgctgggg cggagtattt gccgcacaat atcgtgatag
     7921 gttcggttgg cgcttatgct cggcaaagcg ctgcgcgtga gcgccaccgt tgcgcgcggc
     7981 tgcgcagagc atgtggccat gcgctccggc tacggtgctc aggagcgatt gaaaaaagtt
     8041 ttaaattaaa gtaaaggggt cagttaaagg ggtctgtcct gaatggcagt tagttaaggc
     8101 ctaatgtttt cttggagagt cgtttgcgcc gagtcgtgct gggcaggttc ttccacatcc
     8161 aggcaacggt ctgctgctgc attttacgcg tcaagccgcc ggtaggagat atgaggttga
     8221 ggattctccg cgcccggaaa cgctcacgcg cttttcaccc gcacgggcag aacggaaaat
     8281 ccactggccg ggaaaacggc aaatttcgtt ttcttcgtgg atgaaagcga aatctcagac
     8341 gtgcgggaaa gcagctcctc gaggaaaagt cgtaattcca gccgcgccaa tggcgcgccc
     8401 ggacagatat ggatgcccgc gccccagagc aggtttttgc tctgatcgcg gtccaggcgg
     8461 caggcgtccg gctcttcgaa ggcccgctca tcccgattcg cggcaatcca catcagggtg
     8521 atgcgatcac ccgcattgat cctccgtccg ccgatctcca ccgcacgctt ggcaatgcgc
     8581 cggttgctga ccaagggccc gtgcaggcgc agaatctcct caatggccgc cggcgccaaa
     8641 tccggcccgg cgcgcaaacg attctgcaat tccggatggg tggccaaata atgggtcagg
     8701 atgccggccg aggctgaaat ggtcccaatt tcgcccaccg tccagttgcg caggatgctg
     8761 gcgatttctt cgttgctcag aacacggccc cagactttct cgtgcatcaa tgccgcggta
     8821 aggtccgtct ctggcgcggc ggcggcatcc atccgagcct cgagcagatc gtcaatgatg
     8881 ccctcgaact cgagcgcgat ttccgacatt gccttgcggt cctgagccag ggtggcctcg
     8941 tggttttttc gcgtccagcg caccagcggc ttgtgcaagg tctccggcca gccgagaaac
     9001 gcgcactgca cccgcacggc aaaaggcagc gcaaaggcgg cgatgaattc cgtctctgcc
     9061 tgcgtgatca gctccgtgac cagccgaatc acgatgtccc ggcaaatggg ggcgaacagc
     9121 tccaagtgcc gcgccgagaa gtaggattcg atcacccgtc ggtaggcggt gtgttgcggc
     9181 ggatccatgc cgttcggcac ggataaatgc tgggaaaccg cgttgctgaa cgtcttgtga
     9241 tccagcagga cccgcatcac gtcttcatgg cggaagagcg accaacccat gaattcgctg
     9301 taagccaccg gacagcgacg gcgcatatca tcgtaggccg cccgctggtc gcgctgcacc
     9361 tcgggtgaag tcggatccca gtcgggttgc ggtcggtcgc gtttgctgtc tttcatcgtg
     9421 tgagaaattc ggtgccgcaa tgcgtcgaat aacgtcgcgc gatgattcta cccgatatcg
     9481 gcagcggata aaggaacttt tgggcacggt tctgttttgc ccacggtctt ttcatttgga
     9541 gcggtgcttg acgagtatct ggaaagggat ttggggattg ttctggcagc gctcctgcag
     9601 ggccggccaa gaagcctcga ggtgaaggtc cgccttgagc gcgatcatta aagaacctgt
     9661 cactttattt tgcggctgaa gacctccgcc gcaagacgag ccg
//
LOCUS       DAOQRO010000104         9598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6486_length_9598_cov_5.197841, whole genome shotgun sequence.
ACCESSION   DAOQRO010000104 DAOQRO010000000
VERSION     DAOQRO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6486_length_9598_cov_5.197841"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..697)
                     /locus_tag="PKK20_05565"
     CDS             complement(173..697)
                     /locus_tag="PKK20_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99385.1"
                     /translation="MKINLGCGRKYLPDYVNCDISPHVRADRRFDLDVVPYPFDAEVA
                     DEILLDNVLEHLDDIPRVMAEFHRVLRPGGRLRIYVPYAKTDWALQDPTHKHFFTEHS
                     LNYFIEDHPYNFYSSIRFVLREVRLYADSTTWRHRFRNMLPFRNALRYFLWNMYDGIY
                     FELEKTVARSPGGP"
     gene            complement(743..1006)
                     /locus_tag="PKK20_05570"
     CDS             complement(743..1006)
                     /locus_tag="PKK20_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99386.1"
                     /translation="MRVESPPTLSGAGARGILRLRIRDRSGSGRYVIVLGAAAVVVPV
                     VCDSGGSIAAGILAVGIVAGALILGALVIWNVLALVRSRAARI"
     gene            1103..2533
                     /locus_tag="PKK20_05575"
     CDS             1103..2533
                     /locus_tag="PKK20_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNU99387.1"
                     /translation="MRLPVVFLLVLASGAAWAAAPIALHPANPHYFLWRGQPTVLITS
                     GEHYGALLNRDFNFRTYFDTLAKDGLNNTRTFSGAYCEPQGAFNIARNTLAPQAGALL
                     CPWARSGEPGYANGGNKFDLTRWDDAYFARLDQLLEYASSRGVVVELNLFCPFYEESM
                     WALSPMNARNNVNGVGAVARTNVYTLDRHGGLLGVQEAMVRRIVQAVQKHDNLYYEIC
                     NEPYFGGVTKEWQHRIADVIQAAERDLKTRHLISMNIANGQATVEQPHPAVSIFNFHY
                     AYPPDAVALNFGLDRVIGDNETGFRGTNDLPYRVEAWSFLMAGGGLFNHLDYSFAVGH
                     EDGTFVYGEKQPGGGNPVFRKQMKILGDFLRGFDFIRMKPDTAVIAGGVPARHRAYAL
                     GQPNEAWAVYLARDTKDKQAPTGPQNATLALELPPGAYSVEWLDPKTNRRESKRIEVK
                     AGSGPARLPTPAFEEDIALAVRNLRP"
     gene            complement(2530..3609)
                     /locus_tag="PKK20_05580"
     CDS             complement(2530..3609)
                     /locus_tag="PKK20_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99388.1"
                     /translation="MQQKSDGMNYAPKGQAKAVCEPGEFRFAAIGLDHGHIYGECNGL
                     IEAGGELAWVFDPDPKKVEQFCRTYPGVRPAGSEQQILDDAQVQLVCSACVPCARGPL
                     GVRVMDHGKDYFTDKPPFTSIDALEAARRKSAETGRIFAVYYAERLHVEAAVYAERLI
                     EDGAIGRVVQVLGLGPHRLNAPNRPPWFWDPAQYGGILVDLGSHQIEQFLHYASAQEA
                     RVLHSKIGNYSCPEHPEFEDFGDATLLADTGATFYFRVDWFTPAGLGSWGDGRTMIVG
                     TDGYIELRKYLDIARDPEGDHVYLVDQRGEHHLAVHGKVGFPYFGQLIRDCLDRTQTA
                     MTQRHTFLAAELALKAQAAALRVGP"
     gene            complement(3777..4934)
                     /locus_tag="PKK20_05585"
     CDS             complement(3777..4934)
                     /locus_tag="PKK20_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99389.1"
                     /translation="MIKIAILGAGAIADSHIQSYLKFRERCRIVAVVDLYIEKAAEKV
                     AKHGLAAGLYNDLTAALDGADFDAASVCLPPFEHAKATVDLLRAGKHVLVEKPMATCL
                     EECDAMLEAAKSSGRVLSVVAQNRFKTSMMRLKRVLESGLVGSVLHGQVDSFWWRGGN
                     YYDLWWRGTWEKEGGGCTMNHAVHHIDLFLWMMGVPSWVQSVSLNLAHTNSETEDFST
                     SILGYPNGSVGQITASLVHHGEEQQLAFQGARAKVAVPWQVKAMQPRENGFPDENAET
                     ASEIQAFYDALPVVDHEGHEGQIDNFLEAIEGREALLVDGVQGRQAIELISAIYQSGH
                     SERRVVLPLSADAPFYTRAGILEHARRFHEKTKSVANFATNDITFGRDFGR"
     gene            complement(5085..6155)
                     /locus_tag="PKK20_05590"
     CDS             complement(5085..6155)
                     /locus_tag="PKK20_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU99390.1"
                     /translation="MGVARADVNQAQWEVLRRCARGEDVGHTQVALIVDSPWIPGYLG
                     LSTLDFLTVPEVWLDANLEVERRFPEAIFIPGFWVEMGMATEPSAFGCKVSFYPDRPP
                     AAHPLCDSLDAIEGVAPPNPLTDGLLPLVLNYYRRAEPRVREAGHLIKIVAARGPLTL
                     ATHLVGVSAFLLALRLDPGAAHRLLRITATTARNWLQAQADALHDVEGVLMLDDIAGF
                     VSEKDYLAFAHPYLKEVFDAFPGALKLFHNDTNNPVSYRHVGALGAHIFNFTHEQPLA
                     KVRALTGPDLCLMGNVPPLEVLAQGTPDTVRSKALECMAAHGGRRRLLLSAGGGVSPG
                     TPGENVAALLEAARTEGASWSG"
     gene            complement(6131..7462)
                     /locus_tag="PKK20_05595"
     CDS             complement(6131..7462)
                     /locus_tag="PKK20_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99391.1"
                     /translation="MKSSSGSSPALSRRRFLARGGMLASGAATFPLLLRSGVLAAEAG
                     AGANDRIGIGFIGMGRQAGGLLQEMLRLPAARLVAVADVHLGRAQAVTAKHGGQAFQD
                     YRRLLELKDVDAVITATPEQWRGPICIHSCQAGKDVYAEKPMSLTIREGRLIVQAARK
                     YNRVFQVGSQQRSMSRNRIGCEFIRTGKLGKIKRVIAHNYPTPWEGRLPAQPVPGELD
                     WDMWCGPAPVVPYNKDLFLPRANPGWLSFRPFSGGEMTGWGAHGFDQVQWALGMDESG
                     PIEVWVEGPKFDPPTYTEAEPKARGDKVCSVPKVFFRYAGDIVMELGNGPDGGALFQG
                     EKGEVKIDRGVFESNPEELMVEALRSQPGGGNQSHMKNWLDCIKSRARPIADVETGHR
                     SATVCHLGNIARWAGRRLKWDPVKEVFPDDKDANQYLDRERRKPWVLPEPM"
     gene            complement(7846..9207)
                     /locus_tag="PKK20_05600"
     CDS             complement(7846..9207)
                     /locus_tag="PKK20_05600"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99392.1"
                     /translation="MSRLKIVPHFGSATRRSFLCATVRTGAAAWAGLRFLPRAALGAT
                     DRAAPNERITMGMIGMGRQAYHANLLPFLASPDTQVVAVCDVDAWRLEEGRKRVEEYY
                     AAQTRRGSYKGCMIARAFRNILARSDIDAVMISTPDHWHAIMAIDAARAGKDVALEKP
                     MSLSIAQGRAISDAMVKHNRIFRTDTEVRFSGDFRRLCECVRNGRIGRVRRIRAEVPK
                     EADPVPAQPTMPVPADLDYSLWLGPAPEAPYTEKRVHTRNNLSARPGWMIIQDYCDGI
                     IGNWGTHLLDIVQWGHNSELTGPVEVEGCGEFHPPGGLSDVLREFTVIYRYADGVELE
                     YCMSGRPAVRFEGDDGWIEAVWNKGVEAQPKAILDEPFGPNDLRLPLISEKIDFIRSV
                     KRRTPTLIPAEVGHRTNTMCHLGLIAIKLGLKLKWDPVRERFDDDAANRLLDRPLRAP
                     WSI"
     gene            complement(9204..>9598)
                     /locus_tag="PKK20_05605"
     CDS             complement(9204..>9598)
                     /locus_tag="PKK20_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99393.1"
                     /translation="IVNLAAECLGHPVPCIMGGNTYPILADLLATGTTYLACNVETDQ
                     KRFVESVARTHPHVRVRVNLDPGVVASSELERIHAGVDRVLEIIGDRSNCLIGTGALP
                     FETPPGNVRLIRECLARRASDQSQPSTL"
BASE COUNT         1852 a         3177 c         2879 g         1690 t
ORIGIN      
        1 catctggccc ggtggcgctc ccgccaaaat cggaagtgat ttttgcacag accctaaacg
       61 cggctgcagc agcgcaggga agatcaggca gagcagcacc cccaaatcgc gagggaaaat
      121 gaataatcgc agtgcgcgat tcgagcgcgc gatcgagccg cgcccttcgt tttcacggac
      181 cgccaggcga acgcgcgacg gttttttcga gttcgaaata aatgccgtcg tacatattcc
      241 acaggaaata acgcagcgcg ttccggaacg gcagcatgtt cctgaaccga tggcgccaag
      301 tcgtgctatc ggcatagagc ctcacctcgc ggagcacgaa ccggatgctc gagtaaaagt
      361 tgtaggggtg gtcttcgatg aagtaattca ggctgtgctc ggtgaagaag tgcttgtgcg
      421 ttggatcctg cagcgcccag tctgtcttgg cgtaaggaac atagattcgc agccgacccc
      481 cgggacgcag gacacggtgg aattcggcca tcacccgcgg gatatcatcc agatgctcga
      541 ggacattatc gagcaggatc tcatccgcaa cctccgcatc gaacgggtac ggaaccacat
      601 cgagatcgaa gcgacgatcc gcccggacgt gaggcgagat gtcgcagttc acgtagtccg
      661 gcaggtattt ccgtccgcaa cccaggttga tcttcaagct tgagagaatt ctagggtcgc
      721 ccaggccatc catcaaggcg aatcagatgc gcgcggcgcg cgagcgcacg agcgccagga
      781 cattccagat gaccagtgcc cccaggatca gcgccccggc cacgatcccg accgcgagaa
      841 taccggctgc aatcgacccg cctgaatcgc aaacgacggg cacaacgacg gccgccgcgc
      901 ccaacacgat cacgtagcgc cctgacccgg accgatcgcg gattcgcaag cgaaggatgc
      961 cacgcgcgcc cgctccgctc aacgttggtg gcgattcgac ccgcacggtg ccggtcccga
     1021 cgccaaaaat ctccgccggc caaaaaggag tgcgccccaa aatcggttgc cagcgcaccg
     1081 cagggcggca taggctctcg ccatgcgcct tcccgtcgtc ttcctgctcg ttctggcctc
     1141 cggcgccgca tgggccgccg cgccgatcgc gctgcatccg gcaaaccccc actattttct
     1201 ctggcgcggc cagcccaccg ttctcatcac atccggcgag cattacggcg cgctcttgaa
     1261 ccgggacttt aatttccgga cgtactttga cacactggcg aaggacggtc tgaataacac
     1321 gcgcactttt agcggtgctt actgcgagcc ccagggcgca ttcaacattg cccgcaacac
     1381 tctcgcgccg caggcgggcg ccctgctctg tccctgggcg cgcagcggcg aaccgggcta
     1441 cgccaacggc ggcaacaagt tcgacctgac gcgttgggac gacgcctatt tcgcgcggct
     1501 cgatcagctc ctcgaatacg catcgagccg aggcgttgtg gtggaactga acctgttctg
     1561 tccgttctac gaagaatcga tgtgggcgct cagcccgatg aacgcccgca acaatgtgaa
     1621 cggcgtcggc gccgtcgcgc gaaccaatgt gtacaccctc gaccgccacg gcgggctctt
     1681 gggtgtccag gaagccatgg ttcgaagaat tgtccaagcg gtccagaaac acgacaatct
     1741 ctactacgaa atctgcaacg agccgtactt cggcggcgtg accaaggaat ggcagcaccg
     1801 catcgccgac gtcatccaag cggccgagcg ggacctgaag acccgccacc tgatctcgat
     1861 gaacatcgcc aacggccagg ccacggtgga acaaccacac cccgccgtct cgatcttcaa
     1921 cttccactac gcctatccgc ccgacgccgt cgccctcaac ttcggcctcg atcgagtcat
     1981 cggggacaac gaaaccggtt tccgcggcac gaacgacctg ccctatcggg tcgaggcctg
     2041 gagtttcctc atggcgggcg gcgggctctt caaccacctg gattactcgt ttgcggtcgg
     2101 gcacgaggac ggaacattcg tctacggaga gaagcagccg ggcggtggca acccagtgtt
     2161 tcgaaaacag atgaagattc tcggcgattt cctgcgcggc ttcgacttca tccgcatgaa
     2221 accagacaca gcggtcatcg ctggaggcgt gccggctcgc caccgcgcgt atgccctggg
     2281 ccagccgaac gaagcctggg cggtctacct cgcacgtgac accaaagaca aacaggcgcc
     2341 aaccggcccg caaaacgcga cgctcgcgct cgaactgccg cccggcgcct attccgtcga
     2401 gtggctcgat ccgaagacga acaggcgcga gtcaaagaga atcgaggtca aggccggcag
     2461 cggccccgcg cggctgccaa ccccggcctt cgaggaggat atcgcgctgg ctgttcgaaa
     2521 cctgagaccc taaggcccga cgcgcaacgc cgcggcctgt gccttcaacg ccaattccgc
     2581 ggcgaggaat gtgtggcgct gtgtcatcgc cgtctgagtg cgatcgagac aatcgcgaat
     2641 caactgcccg aagtagggaa acccgacttt accgtggaca gccaggtgat gctcgccccg
     2701 ctggtccacc aggtagacat gatcgccttc gggatcgcgc gcgatgtcga ggtactttcg
     2761 caactcgatg taaccgtcgg tgccgacaat catggttcgg ccgtcgcccc acgaaccgag
     2821 accggcgggc gtaaaccaat ccacgcggaa atagaaggtg gcgcccgtgt cggccagcag
     2881 tgtggcgtcg ccgaaatcct cgaactcggg atgctccggg caactgtagt tgccgatctt
     2941 gctgtggagc acacgggcct cttgcgccga ggcgtagtgg aggaattgct cgatttgatg
     3001 gctgcccaga tcaaccagaa tcccgccgta ttgggccgga tcccagaacc acggggggcg
     3061 gtttggggcg ttcagacgat gcggccccag gccaagcacc tgcaccacac ggccaatggc
     3121 cccatcctcg atgagccgtt cggcgtacac cgcggcctcg acgtgaagac gctcggcata
     3181 gtacacggcg aaaatccgcc cggtctcggc actcttgcgc cgggccgcct cgagcgcatc
     3241 gatcgacgta aacggcggct tgtccgtgaa gtagtccttg ccgtgatcca tcacccgcac
     3301 ccccagcgga ccccgcgcgc aaggaacgca cgcgctgcag acaagctgga cctgagcatc
     3361 atcaagaatc tgctgctcgc tgcctgccgg cctgaccccc ggatacgtcc ggcaaaactg
     3421 ctcgaccttc ttcgggtcgg gatcgaagac ccaagccaat tcgcccccgg cctcgatcaa
     3481 gccgttgcac tcgccataga tgtgcccgtg gtcgagcccg atggccgcaa aacggaactc
     3541 gccgggttcg catacagctt ttgcctgccc cttgggggca tagttcatgc cgtcggattt
     3601 ctgttgcatg gatgatcgcg gttagtcttt tcacggtcgc tatacagagc gtcataagtc
     3661 cgtgacgccg agtcgggcga cccagcctac agggatttgc aggccgcgtg ccctcatgcg
     3721 gcgagacttt tgacgttgtg cacagaccct tagccccggc ggcggttgga gtgagatcat
     3781 ctcccaaaat cccgcccaaa ggtgatgtcg tttgtggcga agtttgcaac gctcttggtc
     3841 ttttcgtgga accgccgggc gtgttcgaga atgccggcgc gcgtgtagaa cggggcgtcc
     3901 gcggacaggg gcagaacaac ccgccgttcg ctgtggcccg actgatagat cgcgctgatc
     3961 aactcgatgg cctggcgtcc ttgcacgcca tcgacaagca gcgcctcgcg cccctcgatc
     4021 gcctcgagga agttgtcaat ctgcccctcg tgcccctcgt ggtccaccac aggcaaggcg
     4081 tcgtagaacg cctggatctc agacgccgtc tccgcattct catccggaaa accgttctct
     4141 ctgggctgca tggccttgac ctgccacggg accgcgacct tggcgcgcgc gccctgaaac
     4201 gccagttgct gttcctcgcc gtgatggacc agcgaggccg tgatctgccc gacactcccg
     4261 ttaggatagc cgaggatgct ggtcgagaaa tcctcggtct ccgagttggt gtgcgccaga
     4321 ttcagcgaca ccgattgcac ccaactcggg acacccatca tccagaggaa gagatcgatg
     4381 tgatgaacgg cgtggttcat ggtgcagccg ccgccctcct tctcccatgt cccccgccac
     4441 cacaggtcgt aatagttgcc cccgcgccac caaaaggaat ccacctggcc gtgcaggacg
     4501 ctgccgacga gacccgattc gaggacgcgc ttgagcctca tcatcgaagt cttgaagcga
     4561 ttctgggcca ctacggacaa gacccgcccg ctcgatttgg cggcctcgag catcgcatcg
     4621 cactcttcga gacaggtagc catcggtttc tcgaccagca cgtgcttgcc ggcccgcagc
     4681 agatcgacag ttgccttggc atgctcgaag ggcggcagac agacggacgc agcgtcgaag
     4741 tccgctccgt cgagcgcggc ggtgagatca ttgtataggc ccgccgccag cccgtgcttc
     4801 gccaccttct ccgcggcctt ctcgatgtag aggtccacga ccgcaacaat ccggcaacgc
     4861 tccctgaact tcaggtagga ttggatgtgc gagtcggcga tggcgccggc gcccagaatg
     4921 gcaatcttga tcatatgagt tcaatgggat tgttctcctg agaagagaca tcccctgggc
     4981 agtctttgtc gctgccttga atgaggatga gggcaaggac cacgtttagg gttcacgggt
     5041 ggcgctgaca tccagtgtct cacggctcaa acgaaaccgc agacctatcc gctccaggac
     5101 gcgccctccg tgcgcgccgc ttcgagcagc gccgccacat tctcgcccgg tgtgcccggc
     5161 gacaccccgc ctccagcgga cagcagcaga cgacgccgcc cgccatgggc ggccatgcac
     5221 tcgagcgcct tcgatcgcac cgtgtcgggc gtgccctggg cgaggacctc gagcggcggc
     5281 acattcccca tcagacagag atcggggccg gttaaagcgc gcacctttgc caagggctgc
     5341 tcatgcgtga aattgaagat gtgcgcaccc agggcgccaa cgtggcgata ggacacaggg
     5401 ttgttggtgt cgttgtggaa cagcttgagc gcccccggga acgcgtcgaa cacctccttg
     5461 agatacgggt gggcgaacgc caggtagtcc ttttctgaga cgaagcccgc gatgtcatcg
     5521 agcatcagca cgccttccac gtcgtgcagc gcgtccgctt gcgcctgcag ccagttgcgg
     5581 gccgtcgtcg cggtgatccg cagcagacga tgcgcggcgc cgggatccag acgcaacgcc
     5641 aggagaaagg cgctcacgcc caccaggtgt gtcgccaaag tcaacgggcc cctcgcggca
     5701 acgatcttga tcaggtgccc agcctccctg acccgcggct cggcccggcg atagtaattg
     5761 agcaccaggg gcagcaaccc gtcagtgagc ggattgggcg gcgccacacc ctcgatagca
     5821 tccagcgaat cgcagagcgg gtgcgccgcg ggcggacggt ccggataaaa actcaccttg
     5881 cagccgaacg cgctcggttc ggtcgccatc cccatctcga cccagaagcc cgggatgaaa
     5941 atcgcctcgg gaaaacgccg ctcgacctcg agattcgcat cgagccacac ctcgggcacc
     6001 gtgagaaaat cgagcgtcga aaggcccaga taacccggta tccacgggct gtcgacgatc
     6061 aaagcgacct gtgtgtggcc gacgtcttcg ccgcgggcgc aacggcgaag cacttcccat
     6121 tgcgcctggt tcacatcggc tcgggcaaca cccacggttt gcgacgctcg cgatcgagat
     6181 actgattcgc gtccttgtcg tccggaaaga cctccttgac cggatcccac ttcaaccggc
     6241 gcccggccca gcgggcgatg ttgccgaggt gacacacggt ggccgagcga tggccggtct
     6301 cgacgtcggc gatcggcctg gcgcggctct tgatgcagtc gagccagttc ttcatgtggc
     6361 tctggttgcc gccacccggt tggctgcgca gcgcctcgac catcaactcc tcgggattcg
     6421 actcgaacac gcctcgatca atcttcacct cgcccttctc gccctggaac agcgcgccgc
     6481 catcaggccc gttgcccagt tccatgacaa tgtcgccggc gtatcggaag aagaccttgg
     6541 gcacactgca gaccttgtcc cctcgagcct tgggttccgc ctcggtgtac gtgggcggat
     6601 cgaacttcgg gccctcgacc cagacctcga tcgggccgct ttcgtccatc cccagcgccc
     6661 attgcacctg gtcaaatcca tgcgcccccc atccggtcat ctcgccgccc gagaacggac
     6721 gaaacgagag ccagccggga ttggctcgcg gcagaaacaa atccttgttg tacggcacga
     6781 ccggcgcagg tccgcaccac atgtcccaat cgagctcgcc aggcaccggt tgagccggca
     6841 gccttccctc ccagggcgtg ggataattgt gggcgatgac gcgtttgatc ttgccaagct
     6901 tgccggtccg gatgaactcg cacccgatgc ggttgcggct catcgaccgc tgctggctgc
     6961 cgacctggaa aacgcgattg tacttccgcg cagcctgaac gatcagccgc ccctcgcgaa
     7021 tggtgagcga catcggcttc tcggcgtaca catccttccc cgcctggcag gagtgaatgc
     7081 agatcgggcc gcgccactgt tccggcgtcg ccgtgatcac cgcatccacg tccttcaatt
     7141 ccagcagacg ccgataatcc tgaaaggcct gaccgccatg tttggccgtg acggcttgcg
     7201 cgcggcccag gtggacgtcc gccacggcga ccagccgcgc cgccggcaga cgcagcatct
     7261 cctggagcag gcccccggcc tgccggccca tcccgatgaa gccgatgccg atccgatcgt
     7321 tggcgccggc ccccgcctca gcggccaaca cacccgaacg caacagcaac ggaaacgtag
     7381 ccgctcctga agcaagcatg ccgccgcgtg ccagaaagcg tcgacgcgac aatgcggggg
     7441 aagacccaga acttgatttc ataagagcat tatggcggtg aatcgtgcca acgaacagcg
     7501 gaaaaaccaa gaacctgctc gttcgttgcg gtgcatccga cagttgtcgg tattctgtcg
     7561 cccggtgagg gcaccgggcc tgcaacggat ggagactccc gctataggcc gcgtgccctg
     7621 cctgcgccgc aaagcgcagc ggtctgtctg ccgtgtcggg cacggcttag gcaggcgcgg
     7681 caggcaggcc cgatgcggcg caaactccat tcccaccgac aacttacaga tgtaccgtac
     7741 tcgttgaacg ttgaacgttg agcgctgggc gctgggcttt gaaccttccc ggttgatggt
     7801 ctgcgtccgc attcaaccgc tgcatgtcaa cgcttgccat cgtcttcaaa tggaccaagg
     7861 cgcgcggagc gggcgatcga gcaaacggtt ggcagcgtcg tcgtcgaacc gttcgcgcac
     7921 cgggtcccac ttgagcttta gtcccagctt gatcgcaatc agccccaggt gacacatcgt
     7981 gttggtccga tggccgactt cagcgggaat cagcgtcggc gttcgcctct tcaccgatcg
     8041 gatgaagtcg atcttctcgc tgatcaacgg gaggcgcagg tcgtttggcc cgaacggttc
     8101 atctagaatc gccttgggct gcgcctcgac tcccttgttc cagactgcct cgatccaccc
     8161 gtcgtcgccc tcgaagcgaa cagccgggcg cccgctcatg cagtactcga gttcgacccc
     8221 gtcggcgtac cgatagatca cggtgaactc gcgcaataca tctgacagac cgccgggcgg
     8281 atgaaactca ccgcatcctt ccacctcgac cggccccgtc aattcgctgt tgtggcccca
     8341 ttggacaata tcgagcagat gcgtgcccca gttcccgatg atcccgtcgc agtagtcctg
     8401 aatgatcatc caaccgggac gcgccgagag gttgttgcgg gtgtgaaccc gcttctccgt
     8461 gtagggcgct tcgggcgccg ggccaagcca caggctgtag tccaggtcgg ccggcacggg
     8521 catcgtcggc tgggcgggca cagggtccgc ctcctttgga acctcggcgc ggatgcgccg
     8581 cacgcgcccg atccggccgt tccggacaca ctcgcacagc cgacgaaagt cgcccgagaa
     8641 tcggacctcg gtgtccgtgc ggaaaatccg gttgtgtttg accatcgcgt cgctgatggc
     8701 gcgcccctgc gcaatcgaca gagacatcgg tttctcgagt gccacgtcct tgccggcacg
     8761 cgcggcatcg atcgccatga tcgcgtgcca atggtcgggc gtcgatatca tgaccgcgtc
     8821 gatgtcgctg cgggccaaga tgttgcggaa cgcgcgggcg atcatgcatc ccttgtacga
     8881 accgcgcctc gtttgcgcgg cgtagtactc ctcgacccgt ttgcgaccct cttcgagccg
     8941 ccaggcatcc acatcgcaca ccgcaaccac ctgggtgtcg ggcgacgcca ggaacggcag
     9001 cagattggcg tgataggcct gccgccccat gccgatcatc cccatcgtaa tccgctcgtt
     9061 gggcgccgcg cgatcggtcg cgcccaaggc ggcccgggga aggaacctca ggccggccca
     9121 ggcggcagcg ccggtccgga ccgttgcgca caggaaactc cgtcgagtcg cagacccgaa
     9181 gtgtgggacg atcttgagac gactcataag gttgagggtt gggattggtc tgaggcgcgt
     9241 ctcgccaggc actcgcgaat caggcgcacg ttcccgggcg gggtctcgaa gggcaaggca
     9301 cccgtgccaa ttaggcagtt ggaccggtcc ccgatgattt cgagaacgcg atccaccccc
     9361 gcgtgtatcc gttcgagttc ggacgaagcg accacgcccg gatcgagatt gacacggacg
     9421 cgaacgtggg gatgggtccg ggccaccgac tcgacgaaac gtttttggtc cgtttccaca
     9481 ttgcaggcca ggtaggtcgt ccccgtggcg agcaaatcgg cgagaatcgg gtatgtgttt
     9541 ccccccatga tgcagggaac ggggtgtccc agacactcgg cggccagatt gacgatgc
//
LOCUS       DAOQRO010000105         9526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6564_length_9526_cov_4.592440, whole genome shotgun sequence.
ACCESSION   DAOQRO010000105 DAOQRO010000000
VERSION     DAOQRO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6564_length_9526_cov_4.592440"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKK20_05610"
     CDS             complement(<1..247)
                     /locus_tag="PKK20_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99394.1"
                     /translation="MSSFIHSSPAGVPDAAPALTHAPIPWDQIGAKAGADYRGDGLRV
                     TAQDDYMKLNCVFQRLEGEATPEGLWLTSTVTNQVQDR"
     gene            813..1814
                     /locus_tag="PKK20_05615"
     CDS             813..1814
                     /locus_tag="PKK20_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNU99395.1"
                     /translation="MEFSRLAWLLPVDQDCAVDFVRSILLVAAGVAVVSAHAADGSSL
                     AERLGHGATARVLIVNCDDVGMCHAANQAVLEAMERGLVTSGTIMVPCPWFNEIADHA
                     RAHPDKSFGVHLCHTSEWKHYRWGPVCSKDQVPGLVDDEGCLWRSIEQLYAHASPDQA
                     LIEGRAQVRKALAAGIDVTHLDSHMGALQYDPRYMESYLKLAVEYDLPVRMASQSTLE
                     KFGQPGLRARFSEKGILFPDYLIHEELKDESKDVKGFWMRVLGGLRPGVTELYIHAAR
                     PTEELQAITGSWRTRAQEFETFTNDPEVRQLIERQGIVRISYRPIRDLQRRLRAPGR"
     gene            complement(1937..4417)
                     /locus_tag="PKK20_05620"
     CDS             complement(1937..4417)
                     /locus_tag="PKK20_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99396.1"
                     /translation="MFLIWPSARAADSDPAGRLAYWRFEGSAWEDVQGRCPLAAANLT
                     AAPSFDGAALCQSSTNSPACLLIGCADTTGARILAPAQGAIRFLYKPLWASPDTVPRL
                     PRAWPRTPGRWLRLFEAGVQGPQGPFAQWMVSIDPVGTNLIVHTRDAAGAVRTNLQVP
                     ISWTAAAVAPRDTNRSEPVQWREIILNYGPQTCALVVNGVVQQDWLTRGLAGQGLPPL
                     AGSSASEQVFSIGSTLTGELPAEGLFDEVETFDRPLSPLRNYAYLARTGLHAKVTASP
                     PSVELSWFTLGNDRQSLERIEPGNPNPLVLATNLTGMSYTDTRGLRLGGVYMYRLGDQ
                     QLIVGLNQPPTDQRGRVIVLVDRTLASGLRSELDQFLADLAGDGWTVSRHDVPRHDDD
                     AWKTGPVNAAYRADVARIKSLVAADYRAAPDQTCALLLIGHVTIPYSGLAAEDGHIDH
                     NGAWPADAYYGDIQGDWTDETVRTAPSVQNPLLRNIPDDGKFDQHMFPKPKRPNASRA
                     VPGLDLAVGRIDFARLPAFRGTSERNLLRQYLTKNHRYRHKELTFRQSALIGGYFGNP
                     YHNESRVLYDIGNLLAYRLYRPVLETVLDGDALAADASAVWAVQGGYGHPTAIHNSVS
                     MNPALGVKRVATADLAQSSATPKAGFYVLKGSYFGDWNYTEDNFMRAVLALPDSGLAA
                     VWTRFTHWAVELTGLGDPLVPTLLATARGDITVRNCSLLGDPTLRLFVTAPPRRLTAS
                     RAGNGVNLAWTPSPDAAQGYLVYRSGNGLSGPFLRVSASPVAGNAFADLNAPSGPLLY
                     QVRACQLLTTGCGAFTNTSQGAMAEVRR"
     gene            4702..5199
                     /locus_tag="PKK20_05625"
     CDS             4702..5199
                     /locus_tag="PKK20_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99397.1"
                     /translation="MNESEYQDLWATVVQRELNGLEKARLEAWLAAHPDARADWVLDE
                     RLSRCLRRLPDIPPSTNFTAQVLHEVGRSSKRRRAQSAPVWRRWVGALTYGWRSAVAC
                     GVLALLIGVPYYHVRQREALARSLEALPAISLAEVELWRDFDSINSLPAGPVPSVDLL
                     AEAFK"
     gene            5277..6332
                     /locus_tag="PKK20_05630"
     CDS             5277..6332
                     /locus_tag="PKK20_05630"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNU99398.1"
                     /translation="MNRHAASLAVSIGGRLVLLLASGLAGAIIGSIAQTSPTLVSGAS
                     VAGTQMGEQRAVLPPLPPPPDNPIRKFSELLALDAAGREAVLAGKGEWQQRYLRERLA
                     EFDALSDTERAVRLGLLHLRYHLLTLMRTPTAQREARLQDVPEEYRDLIVERLLAWEN
                     LPAEQQQELLQNEAVLSQISWFETGSVKRQAATPNPLSIRAPRELQSDLQRWRQLPES
                     QRARMMRNFSQFFELNDRQRQRTLECVAVPDRARIERAAAALERLPADERMRCLDALN
                     RYAEMSAEERARFLQNAARWQAMSLEERQAWRWVAVHLAPSQSFLPRPPVPPPPMPRV
                     QAGSRAIPAVTNAQASQ"
     gene            complement(6337..6639)
                     /locus_tag="PKK20_05635"
     CDS             complement(6337..6639)
                     /locus_tag="PKK20_05635"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNU99399.1"
                     /translation="MAHRLAARSTAELVPAVVELLSLDRRRAETEVFKAWKTLVEPTL
                     AAHARPTGLRRGTLFVSVDSSVWLAEIVRYRQREILERLHSAFGTALIKTIAFRVG"
     gene            complement(6755..8812)
                     /locus_tag="PKK20_05640"
     CDS             complement(6755..8812)
                     /locus_tag="PKK20_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNU99400.1"
                     /translation="MYFGVDYHPEHWVYPYAGTADNPEARWERDAQLMVAAGVNVVRM
                     GEFCWGLYERQEGEYDFKWMWRVMDIMKLHGIKVMLGTPTAAPPLWLAEKHPEILPID
                     EEGRALHEGTRHAYCMNSDVYWEYARKIVTALANELGKHPQLIAWQIDNGIGGHRTEF
                     SFNEESRHDWHAWLEAKYETIERLNDCLGLRFWGQVATKWDDVPMPRIAPTVHNPALV
                     LDWMRFSSDTCVAFVKMQVDLLHELTPGIPTTTNLRALSRHYDHFDMAEVLDFVSLDS
                     FATLSRRPSENAVEHDIMRSLKKADIKTPDGETGFWVVEEKAGNVNWQKVNSLLRPGV
                     VRLFTYQLISRGATGVFYFFWRSPRIGSEKFYGGMLTHDGRGDNRVYREISQIGEEVK
                     LLAPVLRGTRVVAETCILFSHPNEWKLKQPQQHTKLFRLWDHVMLFYNALHDRNIPVD
                     FARPTEDLSRYKIVFAPSLHLLAGGEADLLKLYVQNGGTLVTTFNTGLVDEHDIAPDT
                     GFPSDLTDLLGLEVQEFDPIPADEENHLVFKGAFPASHLHSARVWCDVIEPKECQVLA
                     TYSKDFYAGRPAVTINTFGLGKAIYIGTMCHQPFYYDLVDWLRQMCSLFPLLKVPDSV
                     EVSMRQKDDTRIYFLLNHAPTPVRITFYKPMHDYLTGNTFTGNYDLPPHGVLVLDERI
                     AKA"
     gene            complement(8990..>9526)
                     /locus_tag="PKK20_05645"
     CDS             complement(8990..>9526)
                     /locus_tag="PKK20_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99401.1"
                     /translation="FGYDDLKAEIDAGYPVLIFLQNYPEKWRSFDEIPRANPSIHGML
                     ATGYYIDDLGTQFVRARTSWALGDQYYRWTSASWVSWTPLPVRGVIAFHPLPRILGFE
                     IKGDEISLHWEGPDAELFDQIKQVARKAHWYVLEKATALNPSDFTPVAPPTTARELTV
                     SACCPGTAFYRLRVVSSP"
BASE COUNT         1705 a         2763 c         3276 g         1782 t
ORIGIN      
        1 agcggtcctg gacttgattg gtcaccgtgg aggtgagcca caacccttcc ggcgtcgctt
       61 ctccttccaa ccgctgaaac acgcagttca gtttcatgta gtcgtcctgc gccgttaccc
      121 gcagaccatc gccgcggtaa tccgccccgg ccttcgcccc gatctgatcc cacggaatcg
      181 gcgcgtgggt gagcgctggc gccgcgtccg gcacccccgc cggggaactg tggatgaatg
      241 aagacatgcc agccaagatg gcggtccagg agaatgatga ggttttgttc atgtggtttg
      301 aatgtaaggg gttaaaaacc agggtagaga gagaaaacgg gaagcgaccg ggaaaaccgc
      361 cgccgcacaa acggcaccgc atggcccggc gggaacgcct agcgcgttcc gccgctgcaa
      421 gggccggcaa acccgccggc atccaccgca gacgacttca aatggttatt ggggtccata
      481 cagaccggtc gcttgtgcgc attcatctga acatctgata acgtgaagta gaattactac
      541 actccggatt tttgtcaacg tactttgggg ggaggaaagg cgggggggaa tcaggccgcc
      601 ggcggtgaaa agagggcttg aacctccgtc gccaggaggg gcggcggcgg ctcaaaatac
      661 aaacagaaac agaaacagaa acgccgcgag cgccagcacg gcgaccaccc accagaacgt
      721 aaaggggtcc atagttctca caagcgacaa tcaagagtcc aatcggtcgc tggccgttgc
      781 ggtcgagccg cgcctggcga ccttgttggc ccgtggagtt ctcccggctt gcctggctgc
      841 ttccggtcga ccaggattgc gcggtggact tcgttcgctc gattcttctg gtggcggccg
      901 gggtggcggt cgtgtctgcc cacgccgccg acggctccag cctggcggag cggctcgggc
      961 acggcgccac ggcccgtgtg ctgattgtca attgcgacga tgtcgggatg tgccatgcgg
     1021 caaaccaggc ggtgctcgag gcgatggagc gggggctggt gacttccggg acgatcatgg
     1081 tgccgtgccc ctggttcaac gagatcgccg accatgcgcg cgcgcacccg gacaagtctt
     1141 ttggcgtcca cctgtgccac acgtccgagt ggaagcatta ccgctggggg ccggtctgct
     1201 cgaaggatca ggtgccgggt ttggtggatg acgaaggctg tttgtggcgc tcgatcgagc
     1261 agctctacgc gcatgcgagt ccggaccagg cattgatcga ggggcgggcc caggttcgca
     1321 aggcgctggc ggcgggtatc gacgtgaccc atctggactc tcacatgggc gcgctccagt
     1381 acgatccgcg gtacatggag tcgtacttga aactggcggt cgagtatgat ctgccggtgc
     1441 ggatggcctc ccagtcgacg ctcgagaaat tcggccagcc tggtttgcgc gcccggttct
     1501 cggagaaggg gattctgttc cccgactact tgattcacga ggagttgaaa gatgagtcca
     1561 aggatgtgaa agggttctgg atgcgggtgt tgggggggct gcgtccgggc gtaacggagc
     1621 tttacattca cgctgcaagg ccgactgagg aactccaggc gatcactggg agctggcgca
     1681 ctcgggctca ggaattcgag acgtttacga acgatccgga ggtacggcaa ctgatcgagc
     1741 ggcagggcat cgtgcggatc agttaccgtc cgatccgcga tctgcagcgg cgactgcgcg
     1801 cgccagggag gtagccgatc cgcgatctgt gcggcacggt ctgcaggatt cgtgtgtgtt
     1861 gttcgccggc acatttcgtc tgctccgtcg gtgcgccggc agaagagtgt gaagggtggg
     1921 aggagtgtga aggaggctat cggcggactt cggccattgc gccctggctg gtgttggtga
     1981 acgccccgca ccccgtggtc aggagttgac aggcacgcac ctggtaaagc agcgggcctg
     2041 agggggcgtt gaggtcggcg aatgcgttgc ccgctacggg cgaggcgctc actcgaagga
     2101 acggcccgct caacccgttc ccagacctgt acaccaaata cccttgagcg gcgtcgggtg
     2161 acggtgtcca ggccagattg acgccattcc cggcacgcga tgcggtcaga cggcgggggg
     2221 gcgcggtgac aaaaaggcgg agagttgggt cgcccaacaa gctgcagttg cggacggtga
     2281 tgtctccgcg cgcggtggcc aggagcgtcg gaacgagcgg atcgcccaat ccggtgagtt
     2341 cgactgccca gtgcgtaaac cgggtccaca cggcggctaa tccggagtcg ggcagggcga
     2401 gcacggcgcg catgaagttg tcctcggtgt agttccaatc tccgaagtaa gaccccttga
     2461 gcacgtagaa tccggctttg ggggtggcgc tggattgcgc gagatcggcg gtggccacgc
     2521 gcttgacgcc gagggccggg ttcatgctga cgctgttgtg gatggcggtg ggatggccat
     2581 acccaccttg aacagcccag acggcgctgg catccgcggc cagcgcgtcc ccgtcgagca
     2641 cggtttcgag gaccgggcga tagaggcgat aggccaggag gttcccgatg tcgtagagca
     2701 ccctgctttc gttgtggtat gggtttccga aatagccccc aatcaacgcg ctttgccgga
     2761 aggtcagttc cttgtgacga tagcggtggt ttttcgtcag gtactgccgc aacaagtttc
     2821 tctcagacgt tccccgaaat gcgggcagcc gggcgaagtc gattcgtccg accgccaggt
     2881 cgaggccggg gaccgcgcga gaggcgttgg gtcgttttgg cttggggaac atgtgttgat
     2941 cgaacttccc gtcatccgga atgttgcgga gcagcgggtt ctggaccgag ggggccgttc
     3001 ggacggtctc gtccgtccaa tcgccttgga tgtcgccgta ataggcatcg gctggccagg
     3061 cgccgttgtg gtcgatgtgg ccgtcttcgg cggcgaggcc cgagtagggg atggtgacgt
     3121 ggccgatgag gaggagggcg cacgtttggt ccggagcggc tcgatagtcg gcggcaacga
     3181 gcgacttgat gcgggcgacg tcggcccgat aggcggcatt gaccggaccc gtcttccagg
     3241 cgtcatcgtc gtggcgcggt acgtcatggc gtgacacagt ccagccgtca cctgccaggt
     3301 cggccaggaa ctgatccagc tccgacctca gaccggaagc cagggtgcga tcgaccagga
     3361 caatgacgcg tccgcgctga tcggttggcg gctggttgag gccgacgatg agttgctggt
     3421 cgcccaggcg atacatgtag acaccgccga gcctgaggcc tcgagtgtcg gtgtatgaca
     3481 tgcctgtgag gttcgtggcc agcaccagcg ggttgggatt gcccggttcg atccgttcga
     3541 gggattggcg gtcgttgccc agagtgaacc aggacaattc gacgctgggc ggcgaggctg
     3601 tgactttggc gtggaggccg gttcgggcga ggtaggcgta gttgcgcaac ggtgagagcg
     3661 ggcggtcgaa ggtctcgacc tcatcgaaca gtccctcggc tggaagctcg ccggtcagtg
     3721 tgcttccgat cgagaagacc tgctcgcttg cagaggaacc ggccagcggg ggcaggccct
     3781 gaccggccag gccgcgtgtc aaccaatcct gctggacgac cccgttgacg accagggcgc
     3841 aggtttgggg tccgtagttg aggatgatct cgcgccattg gaccggctcg ctgcggttcg
     3901 tgtcgcgcgg cgcaaccgct gcggccgtcc agcttatcgg gacctggagg ttggtgcgca
     3961 ccgccccggc ggcgtcccgg gtgtgaacga tgaggttagt gccgaccgga tcgatcgaca
     4021 ccatccactg agcgaaggga ccctggggtc cctggacccc ggcctcgaac aggcgaagcc
     4081 atcgtcctgg agtgcgcggc caggcgcggg gaaggcgcgg cacggtgtcc ggactcgccc
     4141 acagtggttt gtacaggaaa cgaatcgcgc cctgcgcggg ggccaggatg cgggcacccg
     4201 tcgtgtcggc gcagccgatc agcaggcagg ccgggctgtt ggttgacgat tgacacagag
     4261 ccgcgccatc gaagctgggg gctgcagtca ggtttgcggc agcaaggggg caccgcccct
     4321 gaacgtcctc ccatgcggaa ccttcaaacc gccaataggc cagtcggccc gcggggtcgg
     4381 aatcagccgc gcgcgcgctc ggccaaatca gaaacatcac tacaaggagc gtcggggccg
     4441 cacagcccgc ccatcggagg gatgaagaga gcggaccgga gcgcctcgaa tgggcaggcc
     4501 gatcagagtg tcgcacggcg ccagggcgcg ccgaatcagt gcgcagacag ggttggattg
     4561 ggcttcggca gtcgatgacg tgctgcattg gtgcaggtta gagcagaact gggcctttgc
     4621 ctaccccgac aatcgcccga acgtgccccg aaacgcacac ctgaaacgca acttcccgat
     4681 ccttccggtt atacatacga cgtgaatgag tcggagtacc aggacctctg ggcgactgtc
     4741 gttcagcgtg agttgaacgg attggagaag gcccgacttg aggcgtggtt ggccgcccac
     4801 ccggacgccc gtgcggattg ggtattggac gagcggttgt cccgctgttt gcgtcgtctg
     4861 ccggacattc caccatccac gaactttacg gcgcaggtgt tgcatgaggt cggacggtct
     4921 tccaagcggc gtcgcgcgca gagcgcgcca gtttggaggc gatgggtcgg ggcgctgacg
     4981 tacggttggc ggtctgcggt tgcgtgtggg gtgctggccc tgctcatcgg ggttccttac
     5041 tatcatgtgc gccagcgcga ggctttggcg cgcagtctcg aggcccttcc ggcgatcagc
     5101 ctggccgagg tcgagctgtg gcgcgatttc gactccatca acagcctccc tgccgggcct
     5161 gtgccgtcgg tggacctcct ggcggaggct ttcaagtgac cgtgcgctgt gagggtatgc
     5221 atcgttggag gccttgggca aaccgggcgc gccttgggac gatcggcgga gggctaatga
     5281 accgacacgc cgcctcgctc gccgtttcga tagggggtcg cttggtcttg ttgctggctt
     5341 cggggcttgc cggcgcgatc attggatcga tcgcgcaaac ctcgcccaca ctcgtttcgg
     5401 gggcttctgt tgcggggacg cagatgggtg agcagcgggc cgtattgccg cctctcccac
     5461 ccccgccgga caatccgatc aggaaattca gcgaattgct ggctctggac gcggccggtc
     5521 gggaggcggt ccttgcgggc aagggcgagt ggcagcagcg gtatttgcgc gagcgtctgg
     5581 cggagttcga tgccctatcc gacaccgagc gagcggtgcg tttggggctg ctgcacttgc
     5641 ggtaccatct gttgacgctg atgcgaacgc ctacagccca gcgggaggcg cggttgcagg
     5701 acgtgccgga ggaataccgc gacctgattg tcgagcgtct gttggcctgg gagaatctgc
     5761 cggccgaaca gcagcaggag ttgcttcaaa acgaagcggt cctcagccaa atctcgtggt
     5821 tcgagaccgg ctcggtcaaa aggcaggccg cgacgcccaa tcccctttcc atccgagccc
     5881 cccgcgagtt gcagtccgat cttcagcggt ggcggcaact gcccgagagc cagcgcgcgc
     5941 ggatgatgcg caatttcagc cagttcttcg aactcaacga ccggcaaaga cagaggactc
     6001 tcgaatgcgt ggctgtgccg gaccgggcgc gaatcgagcg cgccgcggct gcactcgagc
     6061 ggttgccggc ggacgagcgg atgcggtgtc tcgatgcgct gaatcgctat gccgagatga
     6121 gcgctgagga gcgggctcgg tttcttcaga acgcggcccg ttggcaggcg atgtccttgg
     6181 aagaaagaca agcatggcga tgggtggccg ttcatctcgc gcccagtcag tccttcctgc
     6241 cacgtccgcc cgtgccgcct ccgcccatgc cgagggttca ggcgggcagc agggcgattc
     6301 cggcggtcac caacgcccag gcgtcgcagt gaggcgtcag ccgacgcgga acgcgattgt
     6361 cttgatgagg gcggtcccga aggccgaatg aaggcgctcg agaatctcgc gctgccggta
     6421 acgaacgatc tccgccagcc agacgctgct atcgacggac acgaacaagg tgccgcggcg
     6481 cagtcctgtg ggacgcgcat gtgcggcgag ggtgggctcg acaagtgttt tccatgcctt
     6541 gaaaacctcg gtttcggcgc gtcgccggtc gagactcaga agctcgacaa cggcggggac
     6601 caattcggca gtcgaccggg cagcgaggcg atgagccatc tcgactggag cccagtcgat
     6661 tcccctccat tccgcgagaa cggtgtcgcg ggcggaccgc cggggttcgg gtcgacggag
     6721 cggggcaggg ggtggacccg accgggcggt gcgtctaggc ttttgcaatc cgctcatcga
     6781 gcaccaggac gccgtgcggg gggaggtcgt agttgccggt gaatgtgttg ccggtgaggt
     6841 agtcgtgcat cggcttgtaa aacgtgatgc gcacgggggt cggcgcatgg ttcaggagaa
     6901 agtagattct cgtgtcgtct ttctggcgca tgctgacttc gacgctgtca ggcaccttga
     6961 ggagcgggaa caggctgcac atctgtctga gccagtcgac caggtcgtag tagaatggtt
     7021 gatggcacat tgtgccgatg tagatcgcct tgcccaggcc gaaggtgttg atggtcacgg
     7081 ccgggcggcc ggcatagaag tccttcgagt acgtggccag cacctggcat tccttgggct
     7141 cgatcacatc gcaccaaacg cgggcggagt ggaggtggct cgccgggaac gcccctttga
     7201 agaccagatg gttttcctcg tcggcgggga tcggatcgaa ctcctgcacc tcgaggccca
     7261 ggagatccgt caaatccgag gggaaccctg tgtccggggc gatgtcgtgt tcgtcgacga
     7321 ggccggtatt gaaggtggtg accagcgtgc cgccgttctg gacgtagagc ttgaggaggt
     7381 cggcctcgcc cccggccagc aggtggagcg agggcgcgaa cacgatcttg tagcgggaca
     7441 agtcctccgt ggggcgggcg aagtcgaccg ggatgttgcg gtcgtgcagg gcattgtaga
     7501 acagcatcac gtggtcccag agccggaaga gttttgtgtg ctgctgcggt tgtttgagct
     7561 tccactcatt tgggtggctg aaaaggatgc atgtttcggc gaccacgcgt gtgccgcgca
     7621 ggacgggagc gagcagtttg acctcctcgc cgatctggct gatctctcga tagacccggt
     7681 tgtcgccccg gccatcgtgc gtgagcatcc cgccgtaaaa cttctctgaa ccgatgcgcg
     7741 gtgacctcca gaaaaagtag aaaacgccgg tggcgccgcg cgaaatcagt tggtaggtga
     7801 acaggcgcac gacgcccgga cgcaacaggg aattgacctt ctgccagttg acgttgccgg
     7861 ccttctcctc gaccacccag aagccggttt ctccgtcagg cgtcttgatg tcggccttct
     7921 tgagcgagcg catgatatcg tgctcgacgg cgttctccga aggacggcgg ctgagcgtgg
     7981 caaaactgtc gagcgagacg aaatcgagca cttcggccat gtcgaagtgg tcgtagtggc
     8041 gcgagagcgc ccggaggttg gtggtggtgg ggatgccggg cgtgagttcg tggagaaggt
     8101 cgacctgcat cttcacgaag gcaacgcagg tgtcgctgga gaagcgcatc cagtccagga
     8161 ccagggctgg gttgtggact gtgggcgcga tgcggggcat cgggacatca tcccacttgg
     8221 tggccacctg cccccagaac cgcaggccga ggcagtcgtt gaggcgttcg atggtctcgt
     8281 acttggcctc gagccacgca tgccagtcgt gccgggactc ctcgttgaac gagaactcgg
     8341 tgcgatgtcc gccgatcccg ttgtcgatct gccatgctat cagttggggg tgcttgccca
     8401 gttcattcgc cagggccgtg acgatcttgc gcgcgtactc ccagtacacg tcgctgttca
     8461 tgcagtacgc gtgtcgcgtc ccttcgtgca gagcccggcc ctcctcgtcg atgggcagaa
     8521 tctccgggtg tttctcggcc agccagaggg ggggggcggc cgttggcgtg ccgagcatca
     8581 ccttgatgcc gtgcagcttc atgatgtcca tcacgcgcca catccacttg aaatcgtact
     8641 ccccttcctg gcgctcgtag agcccccaac agaattcccc catgcgcacg acattgaccc
     8701 cggcagccac catcaactgc gcatcgcgtt cccatcgcgc ctcgggattg tccgccgttc
     8761 cagcataggg ataaacccaa tgctccgggt gatagtccac gccaaaatac ataaattgac
     8821 tcgtgccgga tttgactggg aagataggcc ccccgccact taaagcaatc tcaaactcat
     8881 ctcgaaaccg tacctccgcg cctcacgccc tctcatcgca ttgcacacct tggagccgga
     8941 cctgacccgt gccaggcctt cgagggattt cgtggactcg gcccgattgt cagggcgacg
     9001 agaccactcg cagtcggtag aaggccgttc cgggacagca ggcggacaca gtcaattcgc
     9061 gggccgtggt tggaggcgca acgggggtga aatcgctggg attgagcgct gtagcctttt
     9121 cgagcacgta ccagtgtgcc ttccgggcga cctgtttgat ctgatcaaag agttccgcgt
     9181 ccggtccctc ccagtgaaga ctgatctcgt cgcctttgat ctcgaagccc aggattcgag
     9241 gcagcgggtg aaaggcaatc acgcctcgga caggcaaagg cgtccaggag acccaagaag
     9301 cgctggtcca ccgatagtat tggtcgccca gggcccagct tgtgcgtgcc cgaacgaact
     9361 gggtgccgag gtcgtcgatg tagtacccag ttgctagcat cccgtgaatc gacggattgg
     9421 ctcgcgggat ctcgtcgaat gaccgccatt tctccgggta gttctgcagg aagatcagca
     9481 ccggatagcc agcgtcgatc tcggccttca agtcatcgta cccgaa
//
LOCUS       DAOQRO010000106         9487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6612_length_9487_cov_5.319126, whole genome shotgun sequence.
ACCESSION   DAOQRO010000106 DAOQRO010000000
VERSION     DAOQRO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6612_length_9487_cov_5.319126"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKK20_05650"
     CDS             complement(<1..754)
                     /locus_tag="PKK20_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNU99402.1"
                     /translation="MKPQNSRGWRSSLTLLAAGCVGMAAVCAQPVKLADFQFNEGSGR
                     TTASTVNSLVGTLGLPSNPDNIPVIINDTPSGAANDRAVQLRGVGYLLADDSNGPILN
                     VATEPLTVEAWVKWDGTDPDVNNGILAYGASYKLGLTTSGQIRWTLFGIVDVNSDWYL
                     PMDNLWHHVAAAYEPGVGVTIYLDGVGQFVAETRPMRAFQHAWLTIGAENLGNPILAA
                     LDRVRVHKALLSETELDSIATEPKGVLASTLVA"
     gene            1437..2471
                     /locus_tag="PKK20_05655"
     CDS             1437..2471
                     /locus_tag="PKK20_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNU99403.1"
                     /translation="MAVPDNFPSESALDDWLTRPCARLIEFIRTLSSPLVILGAGGKM
                     GPTLAVLARRAAEAAQHPLEVIAASRFGDPRIRVWLEERGVRTCSVDLLEADGVRRLP
                     DAQNVVYMVGLKFGTSKAPAMTWAVNTLVPERVAARYPQARIAAISTGNVYPLAEVRR
                     GGSVETDSLHPLGEYANAAVARERLFEYVSRRQHTPVVLLRLFYAVELRYGVIADLAR
                     QIQAGTPIPLANGWFNCIWQGDANAMIIRGLDLAASPPAVWNLCRPELFSVREVANKL
                     GTFLDRAPEFTGAESQTALLGNAAGLCRILGEPPTPMDTIIRWVADWVRSGGRTLERP
                     THFEVRDGQY"
     gene            2741..3814
                     /locus_tag="PKK20_05660"
     CDS             2741..3814
                     /locus_tag="PKK20_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNU99404.1"
                     /translation="MNDDPQPPEWVRDRLGEGLVIPAHPLALTRRRTLDECRQRALTR
                     YYLAAGAGGLAVGVHTTQFAIRDPRHGLLGPVLELAARTAAEAAGASGRPPVLIAGVC
                     GSTAQAAYEARLARALGYHAGLLSLGALRNAPERALLAHCRAVAREIPLVGFYLQPAV
                     GGRVLPFRFWRRFAEIENVVAIKIAPFNRYQTLDVIRAVAEAGRDDIALYTGNDDTIV
                     ADLLTPFRFRVGRATIERRIVGGLLGHWAVWTRKAVELHGACRRIARTGQRVPPDLLR
                     LGAAVTDANAALFDAAHGFAGCIAGLHEILRRQGLMAGRWCLDTHETLSAGQSDEIGR
                     VCRAYPELNDDDFVRAHRDTWLK"
     gene            3991..5148
                     /locus_tag="PKK20_05665"
     CDS             3991..5148
                     /locus_tag="PKK20_05665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNU99405.1"
                     /translation="MRASQSLTGETRRKQVEFLCSVDGVNDSAYYPPPESAGGWRVLD
                     QPDAIRARGGMNPDELEALGAWLRASDNRSFAAVVIRHGHIVLEVERGNSSKTDARRV
                     ASVSKAVCATVLAIASERSRHGLTPRRMSFEDRAFDFIPWASPLSDARKSAITVRQLF
                     NHTSGICPEATGAPNDGTWEYVLGLSGDARTARLAFDPGTACGYSTHALHHAALVCET
                     VTGTPYDQFAIDALFRPLGIEHWWFQFAEGGERIGRHPTHGLGMPARDLARIAYCMLR
                     GGKWGEKQIIPAWFVQQTGAPTHQVKSPELRWKLNPQVFSHGWELPARHDPATGRNGD
                     GIPADARCKPGSGGQFIAFVPSLDLVVTRQTGSSGDWAYEEYLRRACAACR"
     gene            5162..6808
                     /locus_tag="PKK20_05670"
     CDS             5162..6808
                     /locus_tag="PKK20_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNU99406.1"
                     /translation="MKTSLRELMATSGVAFGTSGARGLADAMTDLVCYAYTTGFLQHF
                     ESRGKPAPGSRAIAVAGDLRPSTGRIMEAVARAAEAMGYRVVWCGRIPSPALALFGLE
                     GQIPAIMVTGSHIPEDRNGIKFNTPSGEILKEDEEGIARQVVAVDDALFDAAGRFAPL
                     LRGPRHTGQNPLTPALSHPTCLPKPGPRQVGEGVRRAGEGDGAAGPTDCFHGSVARIE
                     SVEAGEQYIARYVDFFGSSALEGLRLGVYQHSAVGRDILARILEALGAQIILLGRSEV
                     FIPVDTEAIRPEDVALAEQWAREDGYDALVSTDGDSDRPLLSDERGRWLRGDLAGILC
                     ARFLDADAVCTPVSCNTAVERCGWFREVRRTKIGSPYVIAAMNAASAEGGRRVVGYEA
                     NGGFLLNSHIERQGRTLRALPTRDAVLVILSILLLAKRQGRRVSELAAALPARFTASG
                     RMKNVPPRKSAALLERFSTGSDAADGRELEAVFGALCGTVGTIDRTDGLRVTFGNGEI
                     LHVRASGNAPELRCYSEAGTEERAKRINGEALEILGSIVG"
     gene            7150..>9487
                     /locus_tag="PKK20_05675"
     CDS             7150..>9487
                     /locus_tag="PKK20_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNU99407.1"
                     /translation="MNHRVLARCAQALLILAATVATAFADATPPDWQNPGLTGIRNLP
                     PHATMVICPDIRTALKIGPVCNAERVRSRFYRSLNGEWKYRYGKNHGERVPEFWRRDF
                     DDRAWAAIPVPSNVEMHGYGVPIYVNIPYPWPKPWKPPFVPEDDPNNTVNSYRRTFTV
                     PGDWAGRRVLIAFDGVNSFFYLWVNGQKVGMGKDSRTPVQFDITSFLTPGENVLAVEN
                     FRWCDGSYLEDQDFWRMSGIFRDVYLWSPPNIHIRDFEVKTDLDADYRNATLSVSVTL
                     ENTTDQPADVAVNCRLLDARGQSIASPKLTMRVGPDGSGGQGSITQAIANPAKWTAET
                     PALHKLLLALEDSAGKVLEVIPVNVGFREVEIANGSLLVNGRRILIKGANRHETDPDR
                     GQAVTVEGMLEDIRMMKQHNLNAVRTSHYPNQPAWYDLCDRYGLYLVDEANIESHGMG
                     YGRESLANFPEWKDAHLDRTIRMVERDKNHPSVIIWSLGNEAGNGPNFYATYDWIKQR
                     DPSRPIHHERAGFAPNTDIYCPMYPHPSLLRKYADGERVDGGWGRDFVLEAQSERTRP
                     MILCEYAHAMGNSSGNMWHYWDLIYEKPYLQGGFIWDWVDQALREPVRRNATRTVQPV
                     KRGEPWFWAFGGDYGPPGTPSDQNFLCNGLVSPDRKPHPGLFEVKHIYQSLHCHPADL
                     AARTVKVVNWFDFTNPKDLVGVHWSLTGDGRTLQRGELPGPDLAPRASADLAIPVAPF
                     TPKPGVEYFLELSFRLKSDAPWARRGHEIAWDQIQLPDA"
BASE COUNT         1683 a         3044 c         3095 g         1665 t
ORIGIN      
        1 aggcgacgag cgtgctggca aggacaccct tgggctcggt ggcaatgctg tccagttccg
       61 tttcggaaag cagcgccttg tgcacacgaa cgcgatcgag ggcggcgaga atcgggttgc
      121 caagattctc ggctccaatg gtgagccagg cgtgctggaa agcccgcatc ggccgtgttt
      181 ccgcaacgaa ttgtccgacg ccatcgagat agatcgtgac accgacgccg ggctcgtagg
      241 ccgcggcgac atgatgccag aggttgtcca tcggcagata ccagtcgctg ttgacatcga
      301 cgatgccaaa cagggtccaa cggatttgac cgctggtggt caggcccagt ttataggagg
      361 ccccgtaggc gagaatcccg ttgttcacgt ccgggtccgt gccgtcccat ttcacccacg
      421 cctcgacggt gagcggctcg gtggcaacat tcaggatggg accgttgctg tcgtcggcaa
      481 gcagatagcc cacacccctc agttgcaccg cccggtcgtt ggcggcgccc gacggcgtgt
      541 cgttgatgat cacgggaata ttgtccggat tcgagggcaa gcccaaggtt ccgaccaggc
      601 tgttgacggt gcttgcggtg gttctgcccg agccctcgtt gaactggaaa tcggccagtt
      661 tgacgggctg agcgcagact gcggccatcc ccacacagcc tgctgccagg agtgtcaggg
      721 aggatcgcca tccgcgagaa ttctgaggtt tcatgttcat agttgttagg tcccggttgc
      781 tctttctctt ttctcgtttt gccgcctgca aggcggagaa ctcagggcgc tctccgccag
      841 ccattgttat cgcgaatgcc gtccacgcag gcggcgcggg ccagacttca cgacccggtg
      901 tcggggcggg catgacacac atgccaatcg tgtcggcccg acgctttccc atgaccgagc
      961 agatcgagtc tgcaaccgat tgaccacaca atgcaatgcc gaatgagtga aatcgcaggg
     1021 caccagggcg caaagcgatg ccgctcaccg tgcggctcgg acttcgaggc gtttggtctg
     1081 gccggcatcg agtgacacct tgaatccacg catcaactcg acacccaggg ggcgcgacga
     1141 ctatacggtc cccagcaggt cggaatcgct aagacgatcg agccgccggg cggcccgttg
     1201 ctcgatgcgc ggcatgtcga gcgtccgacc ctcgagacac agtgcatctg catcttctca
     1261 gtgaaaatcg agtttgcgcc gcacggggtc tggattgacg cgcacgcgcg gctgcccgaa
     1321 aatgtccgcg gttcaccgcc accttccgaa gatgctgacc ggcggcggac cttgccggcc
     1381 gctgcccgag agacgtcgct aagacaagag caagagcaag aagaacgaga gcgcccatgg
     1441 ctgttcccga caattttccg agtgaatccg ccctcgacga ctggctgaca cggccgtgcg
     1501 cacggctgat cgagttcatc cggacactct cgagtccgct ggtgattctc ggtgccgggg
     1561 ggaagatggg gccgacgctt gccgtgctgg cccgccgggc ggcagaggct gcgcagcacc
     1621 cgctcgaggt gattgcggcg agccgttttg gcgatccgcg catccgggtc tggctcgaag
     1681 aaagaggtgt ccgaacgtgc tctgtcgatc tgctcgaagc ggacggcgtg cggcgtctgc
     1741 cggatgcgca gaacgtggtg tacatggttg gactgaagtt cggcacgtcc aaggcgccgg
     1801 cgatgacgtg ggctgtaaac accctcgtgc cggagcgtgt ggcagcgcgt tacccccagg
     1861 cgcgcatcgc cgccatctcg accgggaatg tctacccgct ggccgaggtc cggcgcggcg
     1921 ggtcggtcga gacggattcg ctgcatccgc tgggcgagta tgccaacgcc gctgtggctc
     1981 gggaacgcct cttcgaatac gtctcccgcc gtcaacatac gccggtcgtg ctgctgcgcc
     2041 tgttctatgc agtcgaactg cggtacggcg tcatcgccga tctcgcccgc caaattcagg
     2101 ctggcacccc aatcccgctg gccaatggct ggttcaactg catttggcag ggtgatgcca
     2161 acgccatgat catccgcggg ctcgatctgg ccgcttcccc acccgcggtc tggaacctgt
     2221 gccgccctga actcttctcc gtccgcgaag tcgccaacaa gctggggacg tttcttgacc
     2281 gcgcgccgga gttcaccggt gcggagtccc agaccgccct gctcggcaac gccgcgggtc
     2341 tgtgccgcat cctgggtgaa cccccgactc ccatggacac gatcatccgc tgggtggcgg
     2401 attgggtgag aagcggcggg cgcactctcg agagaccgac ccatttcgag gtgcgggacg
     2461 gacagtatta gcgaggcttg gcctaagggt ctgtgcaaaa ttacttccga ttttgctgga
     2521 ggggatttgg ctcgccggcg aggcgcgacg aaggagcatt gccttgacct ctgtgatatg
     2581 cagggctatc ccgcatgagt ccacacttcg cgtttttggg cttagccgtg attgagactc
     2641 cacgaaacaa gcagccaacg aatccttgac gccgtcacca caccacagaa actggaccgg
     2701 ccagcaacga gtatgaccgg tcaaggaaaa tgggcagagg atgaatgacg atccgcagcc
     2761 tccggaatgg gtgcgcgaca ggctgggcga gggattggtg attccggcgc atccgctggc
     2821 cctgacccgc cggcgcacat tggacgaatg ccggcagcgg gcgctgacgc gctattacct
     2881 tgccgcgggc gcgggcggtc tcgcggtcgg cgtccatacc acccagttcg caattcgtga
     2941 tccgcggcat ggactcctcg gacccgtcct cgagcttgcc gctcgaaccg ccgccgaggc
     3001 cgctggcgca tcgggccggc cgccagtcct gatcgccggt gtttgcggct cgacggctca
     3061 agcggcatac gaagcccggc tcgcccgcgc cctcggctac catgcggggt tgctttcgct
     3121 gggcgctctc cgcaacgcgc ccgaacgcgc cctgctagcc cattgccggg cggtggcgcg
     3181 cgagattccg ttggtcggat tctatttgca gccggcggtg ggcgggcgcg tcctgccgtt
     3241 ccgcttctgg cgccggttcg cggagatcga gaatgtggtc gccatcaaga tcgccccatt
     3301 caaccgctat cagactctgg atgtgatccg cgccgttgcg gaagcgggcc gagacgacat
     3361 cgcgctgtac acggggaacg acgatacgat cgtggcggac ctgctcactc cgtttcgctt
     3421 ccgcgtcggc cgcgccacga tcgaacgccg gatcgtcggg ggattgctgg gacactgggc
     3481 ggtgtggaca cgcaaggccg tcgaattgca cggcgcgtgt cgccgcatcg cccgtacggg
     3541 gcagcgcgtg ccgccggacc tgctccggct cggggccgcg gtgaccgatg ccaacgcggc
     3601 gctgttcgac gccgctcatg gctttgcggg ctgcattgcg ggtctccacg agattctgcg
     3661 ccgccaaggg ctcatggccg gacgatggtg cctggacacg catgaaacgc tttccgccgg
     3721 acaaagcgat gagatcggtc gcgtctgccg cgcgtatccg gagttgaacg acgacgactt
     3781 cgtgcgagcg caccgcgaca catggctcaa atgaagagga aacaaatgca tccgttcttc
     3841 ggcgatcgtt gccggttcgg gcgcggcgcg tggctgattg ttctgccgtg cgtgtggctc
     3901 ggtgtaggcg ccggccagac atcgccccta gcgcgcattt ctcaccaact cctgaagaat
     3961 gcggagccga tgtcatcgaa aggtgcactc atgcgcgcca gccaatcctt gaccggtgaa
     4021 actcgccgta aacaagtcga gtttctgtgc tccgttgacg gggtcaatga ttctgcgtac
     4081 tacccgccgc ccgaaagcgc cggcgggtgg cgggttctgg atcagccgga cgccatccgc
     4141 gcgcgcggcg ggatgaatcc ggacgagctc gaagcactcg gggcgtggct gagggcgagc
     4201 gacaatcgga gcttcgccgc ggtggtgatc cggcacggcc atatcgtgct cgaagtcgag
     4261 cggggcaaca gcagcaagac cgacgcgcgg cgcgtggcct cggtctcgaa ggcggtgtgc
     4321 gcgaccgtcc tggcaatcgc atccgagcgc agccggcacg gactcactcc gaggagaatg
     4381 tcgttcgagg atcgggcttt cgatttcatc ccgtgggcct cgcctctgag cgacgcccgc
     4441 aagagcgcga tcacggtacg tcaactcttc aatcacacat cggggatatg tccggaagcg
     4501 acgggtgcgc ccaatgacgg gacgtgggaa tacgtgctcg ggctcagcgg cgatgcccgg
     4561 acagcgcggc tcgcgttcga tcccggaact gcgtgcggtt actcgaccca tgcgctgcat
     4621 catgcggcgc tggtctgcga gacagtcacc ggcacaccct acgaccagtt tgcgatcgac
     4681 gcgctgttcc gtccgctcgg catcgagcat tggtggtttc agttcgccga gggcggcgag
     4741 cggatcgggc gccatcccac ccatgggttg ggcatgccgg cgcgcgatct ggcccggatc
     4801 gcctattgca tgctgcgggg cggaaaatgg ggcgagaaac agatcatccc ggcctggttc
     4861 gtccaacaaa cgggcgcccc cacccaccag gtgaagagcc ccgaactgcg ctggaagttg
     4921 aatcctcagg tcttttcgca tggctgggaa ttgcccgccc gccatgaccc ggcgaccgga
     4981 cgcaacggcg acggcattcc cgcggatgcg cgctgcaagc cgggatccgg cgggcagttc
     5041 attgcctttg ttccgagcct ggatctggtt gtcacccggc aaaccgggag cagtggggat
     5101 tgggcctacg aagagtacct gcgccgcgcc tgcgccgcct gccgctgaca acttgacacc
     5161 gatgaaaaca agcctgcggg aattgatggc gaccagtggc gtcgcttttg gcacgagcgg
     5221 cgcgcgcggc ctggcggacg cgatgacgga cctcgtctgt tacgcgtaca cgaccggttt
     5281 cttgcagcac ttcgaatccc gaggcaagcc ggctcccggc agccgcgcga ttgccgtggc
     5341 gggcgatctg cgccccagca ccggccgcat catggaagcc gtcgcccgcg ccgccgaggc
     5401 aatgggatat cgggtcgttt ggtgcggcag gattccctcg cccgccctgg ccctgttcgg
     5461 gctcgagggc cagattccgg ccatcatggt gaccggcagt catatccccg aggatcggaa
     5521 cggcatcaag ttcaacacgc cctcaggcga gattctcaag gaggacgaag aggggatcgc
     5581 gaggcaggtc gtcgcggtgg acgacgctct cttcgacgcc gcgggacgtt tcgccccctt
     5641 gctcagaggg ccgcggcata cgggacaaaa ccccctcacc ccggccctct cccatccgac
     5701 ctgcctgccg aaacctgggc ccaggcaggt gggagagggt gtccgaaggg cgggtgaggg
     5761 tgacggtgct gcgggcccca cggattgctt tcatggctcc gtcgcccgca tcgagtcggt
     5821 ggaggcgggc gagcagtaca ttgcacgcta cgtcgatttc tttggaagca gcgcactcga
     5881 gggactccga ttgggggtgt accagcactc ggcggtcgga cgcgacatac tcgcccggat
     5941 actcgaagcg ctcggcgcac agatcatcct tctcggtcgc tcggaggtct tcattcctgt
     6001 ggacaccgaa gccatccgcc ccgaggacgt ggcgctggca gaacaatggg cgcgggaaga
     6061 cggctacgac gccctggtct cgaccgatgg ggacagcgac cgcccgcttc tgagcgatga
     6121 acggggccgt tggctgcgcg gcgatctggc cggcatcctg tgcgcgcggt ttctcgatgc
     6181 ggacgcggtc tgcacgccgg tgagctgcaa cacggccgtc gagagatgcg gttggttcag
     6241 ggaggtgcgc cggacgaaaa tcggctcgcc gtacgtcatc gccgcaatga acgctgcctc
     6301 ggcggaagga gggcgccgtg tggttggata cgaagccaac ggcggttttc ttctcaattc
     6361 ccacatcgaa cggcaaggcc gcacgctgcg cgccctgccg acgcgcgacg ccgtccttgt
     6421 gattctgagc attctgctgc tcgccaagcg ccagggacgg cgcgtctccg agcttgccgc
     6481 ggctcttccg gcccggttca cggccagcgg tcggatgaag aatgtcccac ctcggaagag
     6541 cgccgcgctg ctcgagcgtt tcagcaccgg gagcgacgcg gcggacggcc gggagctcga
     6601 agcggtgttc ggcgccctgt gcggcacggt cgggacgatc gatcggaccg acggtctgcg
     6661 ggtgacattc ggcaacggcg agatcctcca cgtgcgggct tccgggaacg cgccggaact
     6721 ccggtgttac agcgaagcgg gcaccgaaga gcgcgcgaag cggatcaatg gggaggccct
     6781 cgagattctg ggctcgatcg tgggttgagg cccgcagacg ccgtcagtct tcgatgtacc
     6841 agaacgagtg tacgacgcga cgccgtgcgt cgatctccca cagcgctttg aacggcaccc
     6901 gttcgacatg gccgtcgagt agggtgacat tgcttccatt aactcgatga gggtgaaacc
     6961 gcccggcgac cgcgacagcc ggcacggctt ggcgacggag atcgatgcgg cttcgccgtc
     7021 cgtgaatgcg cctgggcgtc gtatctgtgg cgcgacacaa tccgcatccg cggaggaacg
     7081 tcaaggcctg accggacaag aaatcctggc gtgactcggc gaagggatga gggcaatact
     7141 ggtccagtta tgaatcaccg tgttctggct cgctgtgcgc aagcgcttct cattctcgca
     7201 gccaccgtcg cgacggcgtt tgccgacgcc acgccgccgg actggcagaa tcccggactg
     7261 accggcatcc gcaacctgcc gcctcatgcg acgatggtca tttgcccgga cattcggacg
     7321 gcgctcaaga tcgggccggt gtgcaacgcc gagcgcgtcc ggtcgcgctt ttaccgttcg
     7381 ctcaatgggg agtggaagta ccgatacggg aagaatcacg gcgagcgtgt gccggagttc
     7441 tggcgacggg atttcgacga ccgggcgtgg gcggcgatcc cggtgccttc gaacgtcgag
     7501 atgcacggtt acggcgtgcc gatctacgtc aacatcccct atccgtggcc caaaccctgg
     7561 aaaccgccgt tcgtgcccga agacgatccg aacaacaccg tcaactcgta ccgtcgcaca
     7621 ttcaccgtgc cgggcgactg ggccgggcgc cgtgtcctga tcgctttcga cggtgtcaat
     7681 tcgttcttct acctgtgggt caacggacag aaggtcggca tgggcaagga ctcacgcacg
     7741 ccggtccagt tcgacatcac gtccttcctg actcccggcg agaacgtgct cgcagtcgag
     7801 aacttccgct ggtgtgacgg ctcctacctc gaagaccagg atttctggcg catgagcggc
     7861 attttccggg acgtctacct ctggtcgccg ccgaacatcc atatccgcga tttcgaagtc
     7921 aaaaccgacc tcgatgcgga ttaccggaat gcgaccctga gcgtgtccgt cacgctcgag
     7981 aacacgactg accagccggc ggacgtggcg gtgaactgcc gcctgctcga cgcccgcggc
     8041 cagagcatcg cctcgcccaa gctcacaatg cgcgtcgggc ccgacggcag cggcggacag
     8101 gggagcatca cgcaagcgat cgcgaatccg gcgaagtgga cggcggagac gcctgccctg
     8161 cacaagctgt tgctcgcgct cgaagacagc gctggcaagg tgctggaagt catcccggtc
     8221 aacgtcggct tccgcgaggt cgagatcgcc aacggcagcc tgctcgtcaa cggccggcgc
     8281 atcctgatca agggggccaa ccgccacgaa accgatccgg accgcggcca ggccgtcacc
     8341 gtcgagggta tgctcgaaga catccggatg atgaagcagc acaatctcaa cgccgtgcgc
     8401 acttcgcatt acccgaatca gccggcctgg tatgacttgt gcgaccgcta tgggctctac
     8461 ctcgtcgacg aggccaacat cgaaagccac ggcatgggct acggccgcga atcgctggcc
     8521 aatttccccg agtggaagga cgcgcatctc gaccggacca tccggatggt cgagcgcgac
     8581 aagaaccatc cctcggtgat catctggtcg ctgggcaatg aggccggcaa tggcccgaac
     8641 ttctacgcca cctacgattg gatcaaacag cgcgatccga gccgccccat ccaccacgaa
     8701 cgggccgggt tcgcccccaa caccgacatc tattgtccca tgtatcccca cccgtccctg
     8761 ctccgcaagt acgccgacgg cgagcgtgtc gacgggggct ggggacgcga cttcgtcctc
     8821 gaagcgcaga gcgagcggac gcgtccgatg atcctctgcg agtacgcgca cgcgatgggg
     8881 aacagcagcg gcaacatgtg gcattactgg gacttgatct acgagaagcc ctaccttcag
     8941 ggcggcttca tctgggactg ggtcgaccag gccctccggg agccggttcg ccgcaacgca
     9001 acgcggaccg tgcaacccgt gaagagaggc gagccgtggt tctgggcctt cggcggcgat
     9061 tacggcccgc ccggcacgcc gagcgatcag aacttcctgt gcaacggtct ggtctccccc
     9121 gaccgcaaac cccaccccgg cctgttcgaa gtcaagcaca tctaccagtc cctgcactgc
     9181 catcccgccg atctggccgc gcgcacggtc aaggtcgtca actggttcga tttcacgaat
     9241 cccaaagacc tcgtgggggt gcattggagc ctgaccggcg acggacggac gttgcagcgc
     9301 ggcgagttgc ccgggccgga tcttgcgccg cgggcgtccg ccgatctcgc gattcccgtt
     9361 gcccctttca cgcccaagcc cggcgtcgag tacttcctcg aactgagctt ccgcctcaag
     9421 agcgatgcgc cgtgggcccg acgcggccac gagattgcct gggaccagat tcagttgccc
     9481 gacgcgg
//
LOCUS       DAOQRO010000107         9467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6642_length_9467_cov_4.473863, whole genome shotgun sequence.
ACCESSION   DAOQRO010000107 DAOQRO010000000
VERSION     DAOQRO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6642_length_9467_cov_4.473863"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1162
                     /locus_tag="PKK20_05680"
     CDS             149..1162
                     /locus_tag="PKK20_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99408.1"
                     /translation="MNKPMCWGIARPLCWLALGALCGVSLWAQAPRGAGPYGEWLVKA
                     EFNGRQMESILAFARNPQGELTGQWISMWGLNDLEDVRFEDGQLTFKQVVRLPNDETM
                     TSRFKGAIANRQLTGTVSSDRGEYSLTGERRPRMSRAVGDWDMTVKMGEREYTGKLII
                     RPGPDNTLRAEWKSERGETTITDVALERNTLRFKRTTKIQDREWSATFEGTLQRDTLT
                     GAFTSERGEAAAEGKRVGAALIGDWILELTTDRGTRKQRLRVNPDFTGLYGAAPIEKI
                     RFEGDQVGFDMVRQFGDRTFEMKFAGKLAGSKLEGELSSDRGNTKVSGTKAVPAMGAR
                     QAR"
     gene            complement(1524..2618)
                     /locus_tag="PKK20_05685"
     CDS             complement(1524..2618)
                     /locus_tag="PKK20_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNU99409.1"
                     /translation="MKWSIKVGRLFGIDVFIHFTFLLLLGFLAYVYWRNTQRIEAAIA
                     GVGFFLVLFGCVLLHELGHALMASRFGVRTHDITLLPIGGVARLEKMPEKPMQEFWVA
                     VAGPAVNVAIAAVLFAWLWLAQALEPVHQLTVIEGSLIERLLVVNIFLVLFNMLPAFP
                     MDGGRVLRALLAMRLGRRRATAIAANIGQAMAILFGILGFFYNPFLVFIAIFVFLGAQ
                     AEASAVEMQSALDGLRVRDGMMTRFRTLSTQDTLDTAVAELLAGSQQDFPVIENGLPV
                     GMLRRNDLVKALSEGRRHAHVTEVMCRQCATVDEAAFLKRAVESMHSLQCATLPVISG
                     GRIVGLLTLENVSEKLMINAALETAGTAGR"
     gene            complement(2648..4210)
                     /locus_tag="PKK20_05690"
     CDS             complement(2648..4210)
                     /locus_tag="PKK20_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNU99410.1"
                     /translation="MSNHDRTPHCHAHCCPHAHASEGGAGISRRSFLSGISGVAVGSM
                     AFAWAPWAGVAGTDGGLPVPPARKPLRVKPVLTYATPQRAPKTSWRPWGGIQTEEDAK
                     AETARILEELNTLRTSADFPVEFLPVTAIRDPKQLEGAADLADADTVLLYAAGGPSHV
                     FQQFRDQGKDVIYFVRHKSGPVSLWYEIVSPIQLRGHSDERVTKNFDEQDVVVDNLGE
                     VLWRLRALCGLRNTMSSRIVAIGGASGWAHPRAPELAKERFKLDIQTVSYPDLAEMIK
                     AARADHAAVALAQKRAEAYLKLPGTTLETDLKFVENAMLLDQIFRSVMKQADCRAITI
                     NECMGTIMPMSETTACLTLSTLNDDGYMAFCESDFVVVPSGILLSNIAGRPVFLNDPT
                     YPHDGLITLAHCTGPRKMDGRTVEPARIMTHFESDYGAAPKVEMRKGQITTNILPGFK
                     ADRWVGLLGEIVDTPFLAICRDQIDIRFKCDSLHLAQRMPGFHWMTGYGDYSKELGYA
                     LKRVGIQWEFLG"
     gene            complement(4596..8513)
                     /locus_tag="PKK20_05695"
     CDS             complement(4596..8513)
                     /locus_tag="PKK20_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99411.1"
                     /translation="MKPPKQFRIPLVAAVALTAWAAATSVQADFASEVLSHAPVGYWR
                     LNETNPMPPEIYAANAGSFGAAGNGRYINVTRTQPGAIPSDPAGRCAVIPNANGGWGG
                     SARVRIPWQADLNTAGAFSVEFWAKPAITSAIACPASSVDFNATPRYGWLFYQGGPGL
                     ADGNGWFFRIYHTGTGGNSLAAVDMTIDTDHWYHVVGVYDGATSIKLYVDGALVATTP
                     VDGTFTPNANRTDVPMTFGGRADGVAGDYGWGGQMDEVAYYTKALSDADVLAHYQAGT
                     AATPPQPYETVIKSSGPAVYLRLDEPPYEPLIAVNSGTLGAAGNGIYQIGTASGLRGP
                     SMPSIAGFDEENKAAGFNGVNGSITIPGLPIDTDTVTMLCWLKRDGTQPARAGIMHNR
                     KVSEPQVKATGLGFMDNGLGLCYNWEDHGEAYNFSPAGFVPPDQAWTFFAVTVEPEHA
                     VMYMGTAAGLIAATNVYAHTPHDFSGTTLELGWDNYQTTRIYRGALDEFALFDKALSY
                     DQVKSLFDAALPGVLSITRAPADPVFEGMSVTFQTSVAGSAPITYQWRKDGQNLAGQT
                     GATLVLENAQVADSGSYDVVATAGGRELVSEALALIVHTSPPIITQTPQSAVRFVNGT
                     VTFRAAAIGSHPFTYQWRFNDAPIPGATGPTLVMKDLTPADAGTYTVVITNPYGAFDA
                     SATLAILSPSKLAATATDLGPIGYWRLDETSGDVAYDYWGGRDGVVRSGTTINGTGPV
                     PPDFQGFDAENKAYSFNNSGMVEILDPLNLNSATITIVAWIKPNGAQDDFDGIVFSRG
                     GNTVAGLDYQTGGQLGYHWNDAAATYNFQSGLYPLDGVWNFVALVVEPTQATIYCDDG
                     TGLFYNVNTVNHAAEEFNGTLRFATDSGTGRFFNGMIDDVVIYDRPLTEAEIASINTA
                     GRTGAYAGPIAATIVTQPKSDIIQIGAPYTLSVQATGSVPLTFQWQKDGTDIPGAFRS
                     SYALAAADYADAGTYTVKVSNAGRSVISLPATLGVMPPPEFINLADGLVLHLKFDGDY
                     QDGSGRGNHGTKVGEPVFVPGKLGQALEYNTITAEGVYNHVTLGTPADLAFGADVDFS
                     VSMWVKYSGLPGDLPFISNTQSSMGGAGFTISPAYNEPGLWWGLNDADAPAPWPGVGY
                     QGGADSVLNDNQWHNIVVAFDRQGDATTYIDGVLKDVRSLAGTWNVTSGTPINIGQAS
                     GTYGEDGTFQIDDVGVWRRALMTYEAQAIYTVGQKGRSFDSAGPVTLTLGKTEAGISL
                     IWQAGTLESAETVQGPYTPVAGAKAPYLTVPTSGTAKFYRVRL"
     gene            complement(8795..>9467)
                     /locus_tag="PKK20_05700"
     CDS             complement(8795..>9467)
                     /locus_tag="PKK20_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99412.1"
                     /translation="RGARTLFAGVFDAGVFKSTDNGRSWTLRNSGLGHPDNRRVHRLI
                     LHADGTLFAGVCAKRSAPGAPLMSEGVGLYRSRDGADTWAKINRSQPLLYVKDFSVHP
                     TDSRRILLGACDAGRGDRSSGLYMTEDGGTTWRRIGREGPQTFGGYFHPQHEGWIYMT
                     LTEGAPGPGLWLSRDHGATWKAFGDLPFSNIQRVEFDPTDSMRLRLATFGGGVWRGPI
                     TPNDP"
BASE COUNT         1687 a         2868 c         3156 g         1756 t
ORIGIN      
        1 gctcgccttc cctgcctgat tccatgctca acccccacca atttgtcctg cacccgtttc
       61 gctgccgccg cggatttggc gcttgtggac gagccgggca gggcttaaaa tcgagggcac
      121 acctaaataa aaaaaccgat tgaggcttat gaacaaacca atgtgctggg gaatcgcgcg
      181 tccgctgtgc tggctggctc tgggcgcgct ttgcggcgtt tctctttggg cccaggcgcc
      241 gcgcggggcc gggccttacg gcgagtggct cgtgaaagcg gagttcaacg ggcggcagat
      301 ggaatcgatc ctcgcctttg cgcggaatcc tcagggcgag ctgacgggtc agtggatcag
      361 tatgtggggt ctgaacgatc tcgaggacgt gcggttcgag gatgggcagc tcaccttcaa
      421 gcaggtggtg cgtctgccca acgacgaaac catgacctcc cggttcaagg gcgccatcgc
      481 caatcgccaa ctgaccggca ccgtgtcgag cgatcgcggc gagtacagcc tgaccggcga
      541 acgccgcccg cgcatgtcgc gcgctgtggg cgattgggac atgacggtca agatggggga
      601 gcgtgagtat accggaaaac tcatcatccg gcccggcccg gacaacacgc tcagggcgga
      661 atggaagagc gaacggggtg agacgaccat tacggacgtc gcgctcgaac ggaacaccct
      721 ccgattcaag cgcacgacca agatacagga ccgcgagtgg tccgccacat tcgagggaac
      781 tctgcagcgc gacacgctga cgggcgcttt cacatccgag cgcggcgagg cggcggctga
      841 aggcaagcgg gtgggggcgg ctctgattgg cgattggatt ttagaactga ccaccgaccg
      901 cggcacgcgc aagcaacggc tgcgggtcaa tcccgatttc accggcctct acggcgcggc
      961 gccgatcgag aagatacgat tcgagggcga tcaggtcgga ttcgacatgg tccggcagtt
     1021 tggcgaccgc acgttcgaga tgaagttcgc gggcaagctg gcgggatcga aactcgaagg
     1081 cgaattgagt tccgaccgcg gcaacaccaa agtgagcggt acgaaggctg tgccggcgat
     1141 gggcgcgcgc caagccaggt aagtcccgcg gcatcggcgc caacccgcat agcggacatc
     1201 ttgctggtcc gtagcgctca gccctgagaa tcgaggaggc gcgtcgcgcg cagccgcttc
     1261 atagcggcgt tcgagcttgc gcgcgcgcgc ctcgccaggc gggctggagc ggtgtgcgtg
     1321 tcacatctgt gtgacgaaaa tcgggacccg ccctacccgg atgtgagact ccgcgaggct
     1381 ctgcaccaat ccctgtacat ttcttctttt catgaaagaa aagattttgg ttgcggcttt
     1441 gtctcgcggc agagcaatcg cccgaaatcc gtggagccgc cgacgcttaa gttgcggctt
     1501 tgccgcggca agaagggtgc gggttagcgg cccgcggtgc cggcggtttc cagcgcggcg
     1561 ttgatcatga gtttctcgct gacgttttcg agcgtgagaa ggccgacgat gcgtccgccg
     1621 gagattacgg gcagcgtggc gcactggagg ctgtgcatgg actcgacggc ccgtttgagg
     1681 aacgcggctt catcgaccgt ggcgcattgg cggcacatga cttcagtcac atgcgcgtgg
     1741 cggcgacctt cggaaagcgc cttgacgagg tcgttgcggc gcagcatgcc gaccggcagt
     1801 ccgttctcga tcaccgggaa gtcctgttgc gatccggcaa gcagctcggc cacggcggtg
     1861 tcgagcgtgt cctgcgtcga gagcgtgcgg aaacgagtca tcattccgtc tcggacccga
     1921 aggccgtcga gcgccgactg catctcaacc gcactggctt cggcctgggc gcccaggaac
     1981 acgaagatgg caatgaagac caggaacgga ttgtagaaga agccgaggat gccaaagagg
     2041 atggccatgg cctggccgat attggcggcg atggccgtgg cgcggcgtcg tccgaggcgc
     2101 atcgccagca aggcgcgcag gacacgcccg ccgtccatcg gaaaggcggg cagcatattg
     2161 aacaggacga ggaagatgtt caccaccagc aagcgttcga tcaatgaacc ctcgatcacg
     2221 gtgagttggt ggaccggttc gagggcctgc gcgagccaga gccaggcgaa cagaacggcg
     2281 gcgatcgcca cgttgaccgc cggtccagcc acggcgaccc agaactcctg catcggtttc
     2341 tcgggcatct tttcgaggcg ggcgacgccg ccgatcggca gcagggtgat gtcgtgagtt
     2401 ctgacgccga agcggctggc catcagcgcg tgtcccaatt cgtgcaggag cacgcaaccg
     2461 aaaaggacca ggaagaaccc gacaccggcg atcgccgcct cgatgcgttg cgtgttgcgc
     2521 caatagacat aagccagaaa gccgagaagc agcaggaacg tgaagtgaat gaagacgtcg
     2581 atcccaaaga gtctcccgac tttgatagac catttcatcg catttctcgt cgtgtccgcc
     2641 cgctcgacta tccgaggaac tcccattgga tgccgacgcg cttgagcgca tagcccagtt
     2701 ccttcgagta atcgccatag ccggtcatcc agtgaaaacc gggcatgcgc tgggccaggt
     2761 gcaggctgtc gcacttgaag cggatgtcaa tctgatcgcg acagatcgcc aggaaggggg
     2821 tgtcgacgat ttcgcccagc aggcccaccc agcgatccgc tttgaagccg ggcaggatgt
     2881 tcgtcgtgat ttggcctttg cgcatctcga ccttcggcgc cgcgccgtag tccgactcga
     2941 aatgcgtcat gatccgcgcc ggttcgactg tccggccatc cattttgcgg gggccggtgc
     3001 agtgagccag agtgatcagg ccatcgtggg ggtaggtggg gtcgttgagg aagacggggc
     3061 gtccggcgat attgctcaac aggatgcccg acggaacgac gacgaaatcc gattcgcaaa
     3121 aggccatgta tccgtcgtcg ttgagggtgc tgagggtcag gcatgcggtg gtttcagaca
     3181 tgggcatgat ggtgcccatg cactcgttga tcgtaatcgc ccggcagtcc gcctgcttca
     3241 taacggaccg gaatatctga tcgagcagca tggcattttc aacgaacttg agatcggtct
     3301 cgagtgtcgt gccaggcaac ttcaaatagg cctcggcgcg cttttgcgcc agcgccacgg
     3361 cggcatgatc cgcccgcgcc gctttgatca tctccgccag gtccgggtag gataccgtct
     3421 ggatatcgag tttgaaacgt tcctttgcca gttcgggagc tctggggtgc gcccaaccgc
     3481 tggcgccgcc gattgcaacg atgcggctgc tcatcgtatt cctcaatccg cacagcgccc
     3541 gcaatctcca gagcacctcg cccaggttgt ccacaacaac atcctgttcg tcgaagttct
     3601 tcgtgacgcg ttcgtccgag tggccccgca actggatggg gctgacgatt tcgtaccaca
     3661 gcgagactgg gccggattta tgcctcacga agtagatgac gtccttgcct tggtcgcgga
     3721 actgctggaa cacgtgcgac ggtccgcccg ccgcgtagag caggaccgtg tcggcgtccg
     3781 ccagatcagc cgcgccctcg agttgcttcg gatcgcgaat ggctgtgaca ggcagaaact
     3841 cgacggggaa atcggcgctc gtccgcagcg tgttcaactc ttcgaggatg cgggcggtct
     3901 cggccttggc gtcttcctcg gtctggatgc cgccccaggg ccgccaactg gtcttgggcg
     3961 cccgctgggg cgtcgcgtaa gtcaacacgg gtttgacccg caacggcttc cgggcaggcg
     4021 gaacaggcaa cccgccatcg gtgccggcca caccggccca cggagcccag gcaaacgcca
     4081 tgctgcccac cgcgacaccg ctgatgccgc tcagaaaact gcggcgcgaa atgcccgcgc
     4141 ctccttcaga ggcatgcgcg tgcggacaac aatgggcgtg gcaatggggt gtgcgatcgt
     4201 ggttcgacat aatgaatcct tccgggcaag agacacctcc tgccattcga tcggatggca
     4261 aggtggctgg tggtcacagg cgtctgttta ccgaatggaa gggcgcccga caacagaaaa
     4321 gtcccgctgt cgagcatagg catttcatcc gtccacaggt tcacgtgcat gcgcagcgcg
     4381 ggaatgagtc cgggccatgt cgctctgaca aggccgagga tcaacgccga cctatcccgc
     4441 gatgggggac ggctacaaac cggttcgtgg tcctcgcgcg cattcaacgg ttgatgcggg
     4501 aacttccggt cagtcctccc gaaaaacgag gcaccctctc cccggctggc ggggagaggg
     4561 tgccgtgcag cgggctgccg gacaggcagc agatgctaga gtctaacccg atagaacttg
     4621 gccgtgccgc tggtgggaac ggtcaggtaa ggggcctttg cgccggcgac cggcgtgtac
     4681 ggaccctgaa cggtctccgc cgattcgagg gtcccggcct gccagatcag cgagattccg
     4741 gcctcggtct tgccgagcgt gagcgtcacg ggtcctgcgc tgtcgaagct ccggcctttc
     4801 tggccgacgg tgtagatggc ctgggcctcg tacgtcatga gtgcgcggcg ccacacgccc
     4861 acatcgtcga tctggaacgt gccgtcttcg ccgtaggttc cgctggcctg gccgatgtta
     4921 atgggtgtgc cgctggtgac gttccatgtg ccggcgagcg accggacgtc cttgagcacg
     4981 ccatcgatgt aggtggttgc atcgccctgg cggtcgaagg ccacgacaat gttgtgccac
     5041 tggttgtcgt tgaggacgga gtcggctccg ccctggtatc cgacgccggg ccacggggcg
     5101 ggggcgtcgg cgtcgttcaa gccccaccag agacccggtt cgttgtaggc cggagagatg
     5161 gtgaagccgg cgccgcccat cgagctctgc gtgttcgaga tgaacggcag atcgcccggc
     5221 agcccggagt atttgaccca catcgagacc gagaagtcga cgtcggctcc gaatgcgagg
     5281 tcggcgggtg ttccgagcgt gacgtgattg tagacgccct cggcggtgat cgtgttgtac
     5341 tcgagggcct ggccaagctt gccgggcaca aagacgggtt ctcccacctt cgtcccgtga
     5401 ttgccccgcc ccgagccgtc ctggtagtcg ccgtcgaact tgagatgcag cacgaggccg
     5461 tcagccaggt tgatgaactc gggaggcggc atcacgccca gcgttgcggg caaactgatc
     5521 accgagcgcc ccgcgttcga caccttgacc gtgtaggttc cggcgtcagc gtaatccgcg
     5581 gctgccagcg cgtagctgct gcggaatgcg cccggaatgt cggttccgtc cttctgccac
     5641 tggaaggtca gcgggacgct gccggtcgcc tgcacgctca gtgtataggg cgcgccgatt
     5701 tggatgatgt cggacttggg ctgcgtgacg atcgtggcgg cgatcggccc ggcgtacgcg
     5761 ccggtccttc ccgcggtgtt gatcgaggcg atctccgctt ccgtcagggg gcggtcgtag
     5821 atgaccacat cgtcgatcat gccgttaaag aagcgtccgg tgccgctgtc ggtggcgaat
     5881 cggagcgtgc cgttgaactc ttcggcggca tggttcacgg tgttgacgtt gtagaagagg
     5941 cccgtgccat cgtcgcagta gatagtggcc tgggtcggct cgaccaccag cgcgacaaag
     6001 ttccatacgc cgtcaagcgg gtacaagccc gactggaagt tgtaggtcgc tgccgcgtcg
     6061 ttccaatggt acccgagctg cccgcccgtt tggtagtcca gtccggcgac cgtgttgccg
     6121 ccgcgcgaga acacaatccc gtcgaagtcg tcctgggccc cgtttggctt gatccaggcc
     6181 acgatggtga tagtggctga gttgaggttg agcgggtcga gtatctcgac catgcccgag
     6241 ttgttgaacg agtacgcctt gttctcggcg tcgaatcctt ggaagtcggg cggcaccgga
     6301 ccggttccgt tgatcgtggt gccgctgcgg accacgccgt cgcggccgcc ccagtaatcg
     6361 taagcgacat ccccgctggt ttcgtccagg cgccagtaac cgatcggccc gaggtcagtc
     6421 gccgtagcgg ccagtttgct cgggctgagg attgccaggg tcgccgaggc gtcaaacgcg
     6481 ccgtaggggt tggtgatcac cacggtgtaa gtgcccgcgt cggccggcgt caggtccttc
     6541 atgacaaggg tcgggccagt ggcgcccggg atgggcgcgt cgttgaatct ccattggtag
     6601 gtgaacgggt ggctgccaat ggccgcggct cggaaggtga cggtgccatt cacaaaacgc
     6661 acggcggact gaggcgtttg agtgatgatc ggcgggctgg tatgcacgat gagcgcgagc
     6721 gcctcgctga ctaactcgcg tccgccggcc gtggcaacga cgtcgtaact gccggaatcc
     6781 gcgacctggg cgttctcaag caccagggtc gcgcccgtct gtccggccaa gttctggccg
     6841 tctttgcgcc actggtaggt gatgggcgcc gaaccggcta cgctggtctg gaatgtgacg
     6901 ctcatgccct cgaagaccgg gtcggcgggg gcgcgggtga tcgagaggac gcccgggagc
     6961 gcggcgtcga acaaggactt gacctggtcg tacgacaacg ccttgtcgaa cagggcgaat
     7021 tcgtctaggg cgccgcgata gatgcgtgtt gtctggtagt tgtcccagcc cagttcgagc
     7081 gtggtgccgc tgaaatcgtg cggcgtgtgc gcgtacacgt tggtggcagc gatgaggccg
     7141 gccgccgtgc ccatgtacat caccgcgtgc tccggctcaa cggtcacggc gaagaacgtc
     7201 caggcttgat ccggcgggac gaatccggcg gggctgaagt tgtaggcctc gccatggtcc
     7261 tcccagttgt aacagagccc cagtccgtta tccatgaagc ccaagccggt cgccttgact
     7321 tgcggctcgg acaccttgcg gttgtgcatg atgcctgccc gggccggctg cgtcccgtcg
     7381 cgtttgagcc agcaaagcat cgtcaccgtg tcggtatcga tcggcagacc ggggatggtg
     7441 atcgacccgt tgacgccgtt gaaaccggcg gccttgttct cttcgtcgaa accggcgatg
     7501 gacggcatgc ttgggccacg tagcccggat gcggttccga tctggtagat tccgttgccg
     7561 gcggcgccga gggtgccgga attgaccgcg atcagcggct cgtacggagg ctcgtccagg
     7621 cgcagataaa cggccggccc ggaactcttg ataacggtct cgtagggctg cggtggggtg
     7681 gctgccgtgc cggcctggta atgggccaga acatcggcgt cgctcagggc cttcgtgtag
     7741 taggcgactt cgtccatctg cccgccccag ccgtagtccc cggccacacc gtcggcccgc
     7801 ccgccaaagg tcatcgggac atccgtgcgg ttggcgttgg gtgtgaacgt gccgtccacg
     7861 ggcgtggtgg ccacaagcgc tccgtcgacg tagagcttga ttgatgtcgc tccatcgtag
     7921 actccaacca cgtgatacca atgatcggtg tcgatcgtca tgtcgacagc cgcaaggctg
     7981 ttcccgcctg ttcccgtgtg gtaaatccgg aagaaccagc cgttgccgtc agccaagccc
     8041 ggtccgcctt ggtagaacaa ccatccgtat cggggcgttg cattgaaatc caccgaagac
     8101 gccggacagg caatcgctga ggtaatcgcg ggtttcgccc agaactcgac cgaaaatgcg
     8161 cccgcggtgt tgagatcggc ttgccaggga atccgcacgc gcgccgagcc gccccatccg
     8221 ccgtttgcat tcgggatcac ggcgcatcgg ccggcgggat cgctcgggat ggcccctggc
     8281 tgagttcggg tcacattgat gtagcggccg ttgccggcgg ctccaaacga gccggcatta
     8341 gctgcgtaaa tctccggcgg catcgggttc gtctcgttga gacgccagta gccgaccggg
     8401 gcatgggaga ggacttctga ggcgaagtcg gcttgaacgc ttgtcgcggc ggcccacgcg
     8461 gtcagcgcga ccgcggccac cagcgggatt cggaactgtt tcggtggttt catagtatct
     8521 gttgcagtct catttactgt tcgcgacccg agacggttcc cctctctctg tcgaggcgcg
     8581 cctctcggac tcgatttctg cccttgcgca ggcagcggca acctcgggtg ccgctcgcga
     8641 gggtttgacg gaacgaagag tagtcgagcc gaaccggtgt gacaagcctc atccggaaca
     8701 tcggtgatcc gagggcgatc cgttcgactt gtgcggtttc gctctgatcg ggcgctgcac
     8761 gaggtggccg ctgcacccgc gggggcggcc ggcttcacgg atcgtttgga gtaatcgggc
     8821 cgcgccagac acccccgccg aaagtggcga gccgaagcct catcgaatcc gtcggatcga
     8881 attcgacgcg ctggatgttc gagaatggca gatcgccgaa ggccttccac gtcgccccgt
     8941 ggtcccgtga aagccacaat cccggacccg gcgcgccttc ggtgagggtc atgtaaatcc
     9001 agccttcgtg ctgcggatgg aagtaacctc cgaaggtctg cggaccctct cgcccgatcc
     9061 ggcgccaggt cgtgcctccg tcctcggtca tgtagaggcc gctcgacctg tccccgcgac
     9121 cggcatcgca tgcgccgagg aggatgcggc ggctgtcagt cggatgcact gaaaagtcct
     9181 tcacgtaaag cagcggttgc gaacggttga tcttcgccca agtgtcggcg ccgtcgcgtg
     9241 accgatacaa gcccacccct tcgctcatga gcggcgcgcc gggggcggac cgtttggcgc
     9301 acacaccggc gaagagcgtg ccgtcggcat gcagaatgag gcgatgcaca cgccggttgt
     9361 cggggtgccc cagaccgctg ttcctgagcg tccaggagcg gccgttatcc gtggacttga
     9421 acacgccggc gtcgaacaca ccggcgaaga gcgtccgcgc gccacgg
//
LOCUS       DAOQRO010000108         9392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6738_length_9392_cov_5.433437, whole genome shotgun sequence.
ACCESSION   DAOQRO010000108 DAOQRO010000000
VERSION     DAOQRO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6738_length_9392_cov_5.433437"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            484..1287
                     /locus_tag="PKK20_05705"
     CDS             484..1287
                     /locus_tag="PKK20_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99413.1"
                     /translation="MKLNGVSRKAGFTLIELLVVIAIIAILAGMLLPALGRAKSKAQG
                     IECMGNYRQLTLAWLMYAEDNGDKLPNAYAGTTPWVTGSLNLNDGGNRSNWDVAQDIK
                     KSPLMPYCGQNTAIWKCPADKSTVSVGGRVWPRVRSMAMNNWFGGTEWTTGWRSYKKL
                     SDLVDPGPTMTWLLLDEREDSINDGEFCVDMTGYPDRPQSWRIIDYPASYHNGAGGFS
                     FADGHAEIRRWKDGRTVPKLKKNQGLDLNVASPNNVDVFWMQERSTRSK"
     gene            complement(1340..2371)
                     /locus_tag="PKK20_05710"
     CDS             complement(1340..2371)
                     /locus_tag="PKK20_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNU99414.1"
                     /translation="MAFTACLLAAANAGESLPTNAVAIVGRLASPKARVVVVEEQGAI
                     VSFNPNLQVIRRMVDRGLTNLTLQPTPQAAWRSLVSPEDVVGLKVYSVPGATSGTRPA
                     VVEGVVQSLLDAGQAPARVIVWDRQIGPLRLAGYFTLAERYGIRIAASTETGYDTNYF
                     YDSAFIGQPVWGDHEFGQRGPGIGRKSYVSRLVTREMTKIINLTPLLNHNMAGVSGNL
                     FSLAMGSVDNTVRFMNDPRQLALAVPEIYALPVLGDRVALNIVDALLAQYHGEARSLL
                     HYTAALNQLRFSVDPVALDVLSIQELERRREFAETPAAKPDLQLYQNAALVEIGVSNP
                     QFIEVEWVK"
     gene            2510..4723
                     /locus_tag="PKK20_05715"
     CDS             2510..4723
                     /locus_tag="PKK20_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99415.1"
                     /translation="MQTIKKWRGASAVDARLSPRGIAGCFLGLLALVVLAGCAPPERR
                     SLLEGERLLRQGRPLEAIEPLQRAVKLLATNAPANAQAWNFLGLAYHYAGQPAGAAQA
                     YQNALARDFNLTVVRYNRGCLFLEQNNLSAAINELTTYTTYQPKDLLGWARLGSAQLR
                     ARLVDQAERSFQRVLDLNPTPAQTAEALNNLGICAALRRRSHDAFEYFNSALRQQTNF
                     PPTLLNQAIVAHQQINDRTLALDRYRAYLAASGPLPHAEAVSNLASQIEGELRLRAAA
                     TSAVPALVLVTNLPAQPTNAALVAATPPQPAPSTTKPPPSPAQPTPSPSPTNIARVAD
                     APVPAPEPAPAELPPPVAPKPPTQTLPTIAAQATPAAPVRTEPAMPTPEPIGTPDAFP
                     APTLAARTEPTPTTEPTPTVEPTLPPVEVVRLESEPEVKAAQDIPVPSRVASATTTVT
                     AAPTAPPPAPVAVPETTTASAPPPARSAADSIPTASRPQEPKKKTLVQRLNPLGWFGG
                     KDKAEKESASRMENAPTPLRDLRSLASESAAARPAQATLPMAAAPSAAPPKPEPPVLL
                     RYTYVRPETPVSGDRARAQEALTRGVQFHTAGQHEEAVAAYRQAVEADPSFFEAQYNL
                     GVAAFDAGVWAEALAAFERSLVLKPDDASARLNFAMSLERANYPVDAARELEFLLARA
                     PETVDAHATLARLCAEKLGDPDKARVHYTKVLQLDPRYPEAAAIRRWLTVHRPRP"
     gene            5023..7860
                     /locus_tag="PKK20_05720"
     CDS             5023..7860
                     /locus_tag="PKK20_05720"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNU99416.1"
                     /translation="MGKELIIAEKPSVAADIAKALGGFKRHEDYYESDRFVLSSAVGH
                     LLELAVPEEFEIARGKWTFTHLPHLPPRFSLAPIERNESRLKVLQKLIRRKDIEGLIN
                     ACDAGREGELIFRNIVGYTQAKQPIRRLWLQSMTPAAIREAFARLRTDAEMRPLADAS
                     VCRAEADWLVGINGTRAMTAFNNKTGGFNKTTVGRVQTPTLAVVVEREQRIRAFRPKD
                     YWEVHGTFEAATGRYEGRWFDERFSKETAGTNGEKDDLKPERIWEQAKAEAIRAKCLG
                     RQGTVTEESKPTTQLSPLLYDLTSLQRDANNRFGFAAASTLKIAQALYEKHKVLTYPR
                     TDSRALPEDYAPTVRGTLESFGDGAYGAFARRILDQGWVRPNKRIFNNAKVSDHFAVI
                     PTPLPPKHLTEAEQKIYDMVARRFLAVFYPAAEFLITTRITRVESEPFKSEGKVMVRP
                     GWLEVYGRQVQDEDAPSLPSIAPNEQVATVDIAVQACQTKPPARFTEATLLSAMEGAG
                     KLVEDDELREAMAEKGLGTPATRAQIIEGLLSENYLLRHGKELQPTAKAFSLMVLLNG
                     LGVEALTKPELTGDWEYKLKQMERGRLPRAAFMEEIRELAADIVRRAKSYEHDTIPGD
                     FGVLSARCPKCSGEVHENYKKFQCQNPACDFGLWKIISGRQFEPDEIGRLIAERQIGP
                     LQGFRSKLGKTFAAVIRLNPEYRIEFDFGKVSPASDSGGPAAAVDFTGKEPVGACPKC
                     RHRVFENRMSYVCEKAVGPKRDCDFRAGAVILQQPVDRAQFAKLLADGKTDLLTGFVS
                     KKTGRRFEAFLVLKDGQVRFEFAPRAPRAKGGGARKKGAEPPAPLDFAGQTPIGTCPK
                     CGAKVFESDTHYLCERTQATTRACKFKTGRVILHRPIERAELAALLANGRTELLTQFI
                     SKSGRPFSAYLVLDENGKVGFEFPAREVQTN"
     gene            7914..8405
                     /locus_tag="PKK20_05725"
     CDS             7914..8405
                     /locus_tag="PKK20_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNU99417.1"
                     /translation="MVAVAADTAFFRLRGQAAAAQSTGFKAAAETLCSQGLRSFVIDL
                     TECQSMDSTFIGVLVGLSRRLRRDRGQPGPVELINTTPQVRRQLDDLFVLDHFTLTEC
                     QLEPGAAFEPVQAGPPNKSQLCRLMIEAHQTLMDVHAANIPKFKQVSEFLEQDLQRLD
                     GQG"
     gene            complement(8402..8779)
                     /locus_tag="PKK20_05730"
     CDS             complement(8402..8779)
                     /locus_tag="PKK20_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNU99418.1"
                     /translation="MAVDFKQLRALAQSEVRVTIAHLPARLRRHAERLPVTYEPRPNS
                     AIVSDGFDPDLLGLFVGIPFGEEESGAIDVPAQIILFLESLWEFAEEDEEVFRDEVVT
                     TYLHELGHYLGLDEIDIEERGLD"
     gene            complement(8886..>9392)
                     /locus_tag="PKK20_05735"
     CDS             complement(8886..>9392)
                     /locus_tag="PKK20_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99419.1"
                     /translation="ILDLPHVYRRHTLHGRVAEAAEVDRIDGMMTKKRGTFRFGIGIR
                     ALAAACLVVLTGCTSLSSRGLEYVGAPRFGPTDPAGIEILQAEPERAHDKLGEAVVNA
                     SVDPAPSVEKIEARLRREAARMGADAVYLVHDRTEAVGAVAMGPWWSQSFSPIQGRIV
                     VAVAIKYR"
BASE COUNT         1810 a         3144 c         2902 g         1536 t
ORIGIN      
        1 cgattagttt ttacacagac cctaagcaag catgagcgcc tccgccgctg acgggggagc
       61 cgatttgccc cctgtggcct ctgtcttcgt ctgcgatgcg atgttttgaa acaagaggcc
      121 gagccaaatc tgaccttctg atgatccctt tgactcgatt cagttgcgcc gcctcagggc
      181 gatcgcagcc aagccgatga ggagggggct ccgatcaacg gatagagact cccgctgcag
      241 gccgcgtggc cagacgcggc ggaccgacgc ccggccaagg aatccgggat cgacgatgca
      301 aaccccgatg caaacccgct tgacccgcct ggccatcgac gcctacggtg cgaggtgaag
      361 attgcctgaa gtcagccgat ggccgcgggc aagcccgcgc caggagcaca ctccagacaa
      421 cacgccaaga cgccgcggcg tccgggcgtc ggactggatc acaacgcgga aagggaacaa
      481 gccatgaagc tgaacggagt ctctcgcaag gcgggtttca cgctgatcga gttgctggtt
      541 gtgatcgcca tcattgcgat cctggcggga atgctgttgc ccgcgctggg tcgggccaaa
      601 tcgaaggcgc aggggatcga gtgcatgggg aactaccggc agcttacttt ggcgtggctg
      661 atgtatgcgg aggacaacgg cgacaagctg ccgaacgcgt acgccggaac gaccccctgg
      721 gtcactggca gcctgaatct gaacgacgga ggcaatcggt ccaattggga tgtcgcgcag
      781 gatatcaaga agagcccgct catgccctac tgcggccaga acaccgccat ctggaaatgc
      841 ccagccgaca agtcgaccgt ctccgttggc gggcgggtct ggccgcgtgt ccggagcatg
      901 gcgatgaaca actggtttgg cggaacggag tggacgaccg ggtggcgcag ctacaagaag
      961 ttgagcgact tggttgatcc gggtccgacg atgacctggc tgctgctgga cgagcgggag
     1021 gacagcatca acgacggcga attctgcgtc gatatgaccg gctacccgga tcgaccgcag
     1081 agctggcgca tcatcgatta ccccgccagc taccacaacg gcgccggcgg cttttcattt
     1141 gccgacggtc atgccgaaat ccgaagatgg aaggacggcc gcaccgtgcc gaagctcaaa
     1201 aagaatcagg gcctggacct gaacgtggct tctcccaaca acgtggacgt tttctggatg
     1261 caggaacgct cgacacgcag caagtagacg gccggcatct gcccgcggcg cggcgtgtcg
     1321 ctcccgcgcg tgccggcggt cacttgaccc actcgacttc gatgaattgg ggattgctga
     1381 cgccgatctc gaccagggcg gcgttttggt agagctggag gtccggcttc gctgcagggg
     1441 tttccgcgaa ctcgcgccgg cgttcgagtt cctgaatcga cagcacgtcc aaggccacgg
     1501 gatcgacgct gaatcgcagt tggttgaggg ccgctgtgta atgcaggaga ctgcgggcct
     1561 cgccgtggta ctgggccaga agggcgtcca cgatgttgag cgccacgcgg tcgcccagaa
     1621 cgggcagcgc ataaatctcc ggcacggcca gcgcaagctg cctgggatcg ttcatgaagc
     1681 gcaccgtgtt gtccacgctc cccatcgcga ggctgaacag gttgcccgac acacccgcca
     1741 tgttgtgatt gagcagcggc gtgagattga tgattttggt catctcgcgg gtgacgagcc
     1801 tcgacacgta ggacttgcgt ccgatacccg gcccccgctg gccaaactcg tgatcgcccc
     1861 agaccggctg cccaatgaag gcgctgtcgt aaaagtagtt tgtgtcgtat ccggtctccg
     1921 tgctcgccgc gatgcgaatt ccgtaccgtt cggccagggt gaaatatccc gccagccgca
     1981 gagggccgat ctgccggtcc cagacaatca cgcgggccgg cgcctgcccc gcatccagga
     2041 gcgattggac cacgccctcg accacagcag ggcgggttcc gctggtcgcg ccgggtacgg
     2101 aatagacttt caggccgaca acatcctcgg gtgataccag gctgcgccag gcggcctggg
     2161 gggtcggttg aagggtgaga ttcgtcaggc cccggtccac catgcgccgg atgacttgca
     2221 gattcggatt gaaggaaacg atggcccctt gctcttcgac gaccacgacg cgcgccttgg
     2281 gcgaggccaa ccgccccacg atggcaaccg cgtttgtggg caggctctcc ccggcgtttg
     2341 ccgcagccaa caggcatgcg gtgaaagcca tctgaaaaga cgatgggaat ggaacggcgg
     2401 gccaatggcc tcgcatcaag tcgctcacta gcctggggac tcgcctcatc aacgcaaatg
     2461 accgtgcctt tcttgactcg tcgggcgggc aatgttacca ccggaccaca tgcagaccat
     2521 caaaaagtgg cgcggcgcct ctgccgtcga tgctcggctc tcgccgcgcg gcatcgccgg
     2581 gtgtttcctt gggctgctgg cgcttgtcgt tttggcgggc tgcgcgccgc ccgaacgccg
     2641 gagcctgctc gaaggcgagc gtttgcttcg gcaggggcgt ccgttggagg ccattgaacc
     2701 gctgcaaaga gcggtcaagc tgctggcgac aaacgcgccg gctaacgccc aggcctggaa
     2761 cttcctcggc ctggcctatc attacgccgg tcaacccgcc ggggcagctc aggcctacca
     2821 aaacgccctc gccagggact tcaatctgac cgttgttcgc tacaaccgcg gctgtctgtt
     2881 cctcgagcag aataacctgt ccgccgccat caacgaactg accacttata ccacctacca
     2941 gcccaaggat ttgctcggct gggccaggct cgggtccgcc cagttgcgcg cgcgcttggt
     3001 cgatcaggcc gagcgaagtt ttcagcgcgt gctcgacttg aatcccaccc ccgctcagac
     3061 cgccgaagcc ctcaacaatc tcggcatttg tgccgccctg cgccgccgat cgcacgatgc
     3121 cttcgagtac ttcaactccg ctctgcggca acagaccaat ttcccgccga ctctgctcaa
     3181 tcaggccatc gtcgcacacc agcagatcaa tgaccgcacc ctcgccctgg accgttaccg
     3241 cgcctacctc gccgcatccg gtcccctccc ccacgccgag gcggtctcga atctcgcaag
     3301 ccagatcgag ggcgagttgc gcctgcgcgc cgccgccacg agcgctgtgc ccgcgctggt
     3361 cctcgtcacg aatctccccg cgcagcccac aaatgcggca cttgtggcgg cgactccccc
     3421 acaacccgcg ccctcaacaa ccaaaccgcc gccgtcaccc gcccaaccga ccccaagccc
     3481 atcgccgacc aatatcgctc gcgtcgccga cgcccctgtc cctgcgcccg agcccgcacc
     3541 ggccgaactg ccccccccgg ttgcccccaa gccgccgact cagactctcc ctaccatcgc
     3601 cgcccaggct acccccgccg cgcccgtccg gacggagccg gcaatgccaa cacccgaacc
     3661 cattgggacg ccggacgcct ttcctgcacc cacgctggcg gcgcggaccg agccaacgcc
     3721 gacgaccgag ccaacgccga cggtcgagcc gactctgccg ccggtcgagg tggtccggct
     3781 tgaatcagag cctgaggtca aggccgccca ggacattccc gttccatcca gggtcgcttc
     3841 cgcgacaaca accgtaaccg ctgcgcccac ggcccccccc cctgcacccg tcgccgtccc
     3901 ggagacgaca acagcatccg cgcccccgcc ggcgaggtcc gccgcggact cgattccgac
     3961 ggcgagtcgg ccgcaggaac ccaagaagaa aacgctcgtc cagagactca atccgctggg
     4021 ctggtttggg ggcaaggaca aggcggaaaa ggagtctgcg tcgaggatgg agaacgcccc
     4081 gacacctttg agggatctga gatcgctcgc gagcgagagt gcggcggcca ggccagcaca
     4141 agccactctg cctatggcgg cagcgccctc cgcggcgccc ccgaaacccg agccaccggt
     4201 tcttcttcgt tatacgtatg tgcgccccga gacgccggtg tccggcgatc gggctcgagc
     4261 ccaggaggcg ctgacgcggg gcgtccagtt ccacacggca ggacagcacg aggaggcggt
     4321 ggccgcgtac agacaagccg tcgaggcgga tccgtcgttc ttcgaggcgc agtacaacct
     4381 cggggtggcg gcgtttgacg ccggcgtttg ggcggaggcc ctcgccgcat tcgagcggtc
     4441 gctggtgttg aagccggacg acgccagcgc gcgactcaac ttcgcaatgt ccctcgaacg
     4501 ggcgaattat cccgtggacg cggcccgcga actcgagttc ctgttggccc gcgcccctga
     4561 gacggtcgat gcccacgcca cgctcgcccg tctgtgcgcc gagaaactgg gtgatcccga
     4621 caaggcgcgt gtgcactaca cgaaggtgct gcagttggat ccccggtatc cggaggccgc
     4681 ggccattcgt cgctggctga cggttcatcg gccgcgcccc tagccttgac cggcaacaca
     4741 tgtcgcagaa aagtcaagtt tctgtgctgc gttaaacgag gcaagaattc taccgggcgg
     4801 ggcgtgggga gagaagggcg gggagacacg gggactcctc atgccaagag gggaggactg
     4861 ggtttggcct gaggcgcgcc tcgcttccac aagacgtcct tcttcgccct gccgcactcc
     4921 gcctttgact tcctgcggct ttttggcgag gttccccccg ctttggaccg aaggggccgc
     4981 ggcccaccag cccgcggcgt gtgcggatcg agggaacaac acatgggaaa ggaattgatc
     5041 attgccgaga aaccgtccgt ggctgccgac atcgcgaagg cgctgggcgg cttcaaacgg
     5101 catgaggact actacgagag cgatcggttc gttctttcgt cggcggtcgg ccacctgctc
     5161 gagttggcgg tgcccgagga gttcgagatc gcgcgcggca aatggacctt tacgcatctg
     5221 ccgcatctgc cgccgcgatt cagtctggcg ccgatcgaga ggaacgaaag ccgactcaaa
     5281 gtgcttcaga aactgattcg gcgaaaggac atcgaggggc tgatcaacgc gtgcgacgcg
     5341 gggcgcgagg gagaactcat cttccgcaat atcgtcgggt acacccaggc caaacagccc
     5401 atccgccgcc tttggttgca gtccatgacg cccgcggcca tccgcgaggc ctttgcgcgg
     5461 cttcgcacgg atgccgagat gcgtccgctg gcggacgcct cggtctgccg ggcggaggcc
     5521 gattggctgg tggggatcaa tggtacccgc gcgatgacgg ccttcaacaa caagactggc
     5581 ggtttcaaca agaccaccgt cgggcgcgtg cagacgccga ccctggctgt cgtggtcgag
     5641 cgcgagcagc ggattcgcgc gtttcggccc aaggactact gggaagtgca cgggacgttc
     5701 gaagcggcga ccggacgcta cgaaggccgc tggttcgacg agcgtttcag caaggagacc
     5761 gccggcacga atggggaaaa ggacgatctg aagccggaac ggatttggga gcaggccaag
     5821 gccgaggcga tccgggccaa gtgcctgggc cggcagggga cggtcaccga ggagagcaag
     5881 ccgaccactc agctctcgcc gctcctgtac gacctgacga gtctgcaacg cgatgccaac
     5941 aaccgtttcg gattcgcggc tgccagcacg ctgaagatcg cccaggccct ctatgaaaag
     6001 cacaaggtcc tgacctatcc gcgcaccgac tcgcgagcgc tcccggagga ttacgccccg
     6061 acggtccggg gaactctcga gtccttcggt gacggcgcct atggcgcttt cgcccgtcgg
     6121 attctggacc agggctgggt caggcccaac aagagaatct tcaacaacgc caaagtctcg
     6181 gaccacttcg ccgtcattcc cacaccgctg ccgcccaagc atctgactga agccgaacag
     6241 aaaatctacg acatggtcgc caggcgattc ctggccgtgt tctatcccgc cgcggagttc
     6301 ctgatcacca cccgaatcac gcgcgtcgag tccgagccgt tcaagagtga aggcaaagtc
     6361 atggtccgac ccggctggct cgaggtctat gggcgccagg tccaggacga ggatgccccg
     6421 agcctgccct cgatcgcgcc caacgaacag gtggccaccg tcgatatagc cgtccaagcc
     6481 tgccagacca aacccccggc gcgattcacc gaagccaccc tcttgtcggc catggaaggc
     6541 gccgggaaac tggtcgagga cgacgagctt cgcgaggcga tggccgagaa gggcctcggg
     6601 acacccgcca cccgagccca aatcatcgag ggtctgctct cggaaaacta cctgctccgg
     6661 cacggcaagg aactgcagcc gaccgcgaag gccttttcgc tcatggtgct gctcaatggc
     6721 ctgggcgtcg aggcgctcac caagcccgag ctgaccggcg attgggaata caaactcaag
     6781 cagatggaac ggggccggct cccgcgcgcc gctttcatgg aggaaatccg ggagctggcc
     6841 gcggacatcg tgcgacgagc caagtcatac gagcatgaca ccatcccggg cgacttcggt
     6901 gtcttgagcg cccgctgccc gaaatgcagc ggcgaagtgc acgagaacta caagaagttc
     6961 caatgccaga accccgcctg cgacttcggc ctctggaaga tcatctccgg acgccagttc
     7021 gaaccggacg aaatcgggcg gttgatcgcg gagcgacaga tcggtccgct gcaggggttt
     7081 cgcagcaagc tgggcaagac cttcgcggcg gtcatcaggc tgaacccgga ataccggatc
     7141 gagttcgact tcggcaaggt cagcccggcg tcagacagcg gcggccccgc cgcagccgtc
     7201 gatttcaccg gcaaggaacc ggtgggagcg tgtccaaagt gccgccatcg ggttttcgaa
     7261 aacaggatga gctatgtctg cgaaaaggcc gtgggcccca aacgcgactg cgatttccgg
     7321 gccggcgcgg tgattctcca acagccggtc gatcgggccc agttcgcaaa gctccttgcc
     7381 gatggaaaga ccgacctgct cacgggcttc gtctcgaaga agacaggacg gagattcgag
     7441 gcgtttctcg tgttgaagga cggtcaggtc cgcttcgagt ttgccccccg cgcaccgcgg
     7501 gccaaaggcg gcggcgctcg gaagaaggga gcggaaccgc ccgcgccgct cgatttcgca
     7561 ggtcaaaccc cgatcggcac atgcccgaaa tgcggcgcaa aggtgttcga atccgatacc
     7621 cattatcttt gcgagcgaac ccaggcgacg accagggcct gcaagttcaa gaccggacgc
     7681 gtgattctgc atcgccccat cgagcgagcg gaactcgcgg cgctcctggc caacggacgc
     7741 accgagttgc tgactcagtt catctccaag tccggacgcc cgttctcggc ctacctggtc
     7801 ctcgacgaaa acggcaaggt cggcttcgag ttcccggcca gggaggtgca aacgaactga
     7861 acgggtccgc cagccgcacc ctgcccctca tccgaatgcc cgccaatcaa gtcatggtcg
     7921 ctgtcgccgc cgacacggcg ttcttccgcc tgcgcggcca ggccgccgcc gcccaatcca
     7981 ccggcttcaa ggcggccgcc gaaaccctct gctcccaggg gctgcggagt ttcgtgatcg
     8041 acttgacgga gtgccagtcc atggacagca ccttcatcgg cgtgctggtc gggctgagcc
     8101 gccggctgcg gcgcgatcgg ggtcagcccg gcccggtcga gttgatcaac accaccccgc
     8161 aggtccggcg gcagttggac gacctctttg tgctcgatca cttcacgctc accgagtgcc
     8221 agctcgagcc cggcgcggcc ttcgaaccgg tccaggccgg tccgcccaac aaaagccagt
     8281 tgtgccgtct catgatcgaa gcccaccaga cgctcatgga tgtccacgcg gcgaacatcc
     8341 cgaagttcaa acaggtctcc gagttcctcg aacaagatct ccagagactc gacggccagg
     8401 gctaatcgag tccccgctcc tcgatgtcga tctcgtccag ccccaggtaa tggcccagtt
     8461 cgtgcaagta ggtcgtcaca acctcgtcgc gaaagacctc ctcgtcttcc tcggcgaact
     8521 cccagagact ttccaggaac agaatgatct gcgccggcac atcgatggcc ccggattcct
     8581 cctcgccgaa agggatgccc acaaacaacc cgagcagatc gggatcgaaa ccgtctgaca
     8641 cgatcgcact gttggggcgc ggctcgtagg tcacgggcag cctctcggca tgccgacgca
     8701 ggcgggccgg caaatgggcg atcgtgacgc gcacctcgga ctgggccaga gcgcgcaact
     8761 gtttgaagtc aacggccacc gggcttgtga gtgctggcct gcatggatcc acgcctcgga
     8821 gattcaggcc ccgcggcggt cacaactcca tgaaacctgt gggctggaat ccgcgggcga
     8881 tcgcctcacc tgtacttgat cgccacagcc accacaatgc gaccttggat cggactgaaa
     8941 ctctggctcc accacggccc cattgcgacc gcgccgaccg cttccgtgcg gtcgtgcacc
     9001 aggtaaacgg cgtccgcccc catgcgggca gcctcgcgac gcagccgggc ctcgatcttt
     9061 tccaccgacg gggccgggtc caccgacgcg ttgaccaccg cctcccccag tttgtcatgc
     9121 gcccgctccg gctccgcctg cagaatctcg atgcccgccg gatcggtagg gccgaatcgg
     9181 ggcgcgccga cgtactcgag accgcgactg gacaacgagg tgcatccggt gagaacaaca
     9241 aggcacgcag ccgcgagcgc tcgaatccca ataccgaacc tgaaagtgcc acgcttcttg
     9301 gtcatcatgc cgtctattct atcgacttcc gccgcctccg caactctccc atgaagcgtg
     9361 tgccttcggt agacatgcgg aaggtcgaga at
//
LOCUS       DAOQRO010000109         9346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6781_length_9346_cov_4.374125, whole genome shotgun sequence.
ACCESSION   DAOQRO010000109 DAOQRO010000000
VERSION     DAOQRO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6781_length_9346_cov_4.374125"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(601..677)
                     /locus_tag="PKK20_05740"
     tRNA            complement(601..677)
                     /locus_tag="PKK20_05740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:Gly,seq:gcc)
     gene            complement(1057..1890)
                     /locus_tag="PKK20_05745"
     CDS             complement(1057..1890)
                     /locus_tag="PKK20_05745"
                     /inference="COORDINATES: protein motif:HMM:NF023384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HNU99420.1"
                     /translation="MPTCLWAADAPAYRAARSVHLGYPAPDGDLFYTEMIIDESVNGS
                     YFMACGWNTGYFGLQQLWKPEDKVAIFSVWDPTRGDDPGAVKSEDRVEVLYQGEGVRI
                     KRFGGEGTGGQCMAPFLWKIGETNRFLLRAEVDGQKTAYTAWLFQPEKRTWWKLATFR
                     TRTGGQRLRGYYSFVEDFRRDGRSALEVRRARYGRGWVRQNADAWTPLAKARFTASGA
                     EWEAKETIDAGLTDGWFHLATGGSTKQSRPLHSIIELPAPPTLPPDWLRLLDGEPGRP
                     D"
     gene            complement(2031..2648)
                     /locus_tag="PKK20_05750"
     CDS             complement(2031..2648)
                     /locus_tag="PKK20_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNU99421.1"
                     /translation="MTSRYILEQFGVGVAAVTGVMAARGKHVDLFGVIVLALVTAFGG
                     GTIRDLVLGDHPVFWAKDPHYVYTAAGAAVVTFFLARTHEIHLRSLQVADAFALALFT
                     IVGLVKACAFHAPAPIAIMLGVTTGVAGGILRDLLLNEIPLVFRPDIYLYATAAFFGA
                     VLYVVLQSARVAHLTNMVISVLAILLARLAGIQWKLALPVFKPKD"
     gene            complement(2664..3518)
                     /locus_tag="PKK20_05755"
     CDS             complement(2664..3518)
                     /locus_tag="PKK20_05755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNU99422.1"
                     /translation="MNYYDAHNHLQDERFGGRQSNLIAACVKAGVRRMVVNGSCPADW
                     PQVLALAREHPQVIPSFGYHPWHLGDRAADWEANLARFLDEIASGVGEIGLDRWKPGL
                     SYESQEEVFLAQLRLAAARAIPASIHCLRAWGRLEQLLAANPRPACGFLLHSYGGPLE
                     MVKPLARLGAYFSFPGYFARPGKERQREVFRAVPPDRLLIETDAPDQCLPSALAQFRL
                     HGPCIEAGAVCSGDFPADAARTSGRPLNHPANLVVVYDYVAGMLGEPIQSLAARVEEN
                     FHRLFRGL"
     gene            complement(3515..4339)
                     /gene="amrB"
                     /locus_tag="PKK20_05760"
     CDS             complement(3515..4339)
                     /gene="amrB"
                     /locus_tag="PKK20_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNU99423.1"
                     /translation="MKADSRSECGRVRPPAQAGRFYPANPAQLRKEVSDYLAAAPRST
                     GPVPKALIAPHAGYMYSGPIAGSAYARLESGRDQIRRVVLVGPSHFARFHGVAASGMD
                     AFETPLGRVPLDRAGIACAAGLPGVAISEDPHREEHSLEVHIPFLQVVLGEFELVPLL
                     VGEATQSEMARLIEVLWGGPESCIVASTDLSHYHNYNTARQFDEATARAIEAFEGERL
                     DGYDACGFQGVRGLLEVARDRGLECRAIDLRNSGDTAGSRSRVVGYGAFVLTAPGQ"
     gene            complement(4336..5487)
                     /gene="amrS"
                     /locus_tag="PKK20_05765"
     CDS             complement(4336..5487)
                     /gene="amrS"
                     /locus_tag="PKK20_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNU99424.1"
                     /translation="MPIPRDEPGQSWVPASSRFAESLDRHTAVGSLWRAEGGKVRCFA
                     CGHRCLIGLGRRGVCKVRLNSAGQLRVPFGYVSGAACDPVEKKPFYHLLPGSGALTFG
                     MFGCDFHCAYCQNWITSQALRDESAGTRIQPITSDELVGAAQRQGARLMVSSYNEPFI
                     TSEWAAEVFARARPAGLVCGFVSNGNATREARDFLEPWLQAVNIDLKTFDDRRYRSLG
                     GTLERVIGTIRDAHARGIWVEVVTLLVPGFNDTEAELRAMARFLVSVSPDIPWHVTAF
                     HPDYRMKEPRATEAGDLVRAAEMGVAEGLRFVYAGNLSGRVGHWEDTRCSGCGQTLVR
                     RRGFRMIEDRLSPRGCCPGCGRSIPGIWAAAGESRRESGMLDRRNALST"
     gene            complement(5700..6401)
                     /locus_tag="PKK20_05770"
     CDS             complement(5700..6401)
                     /locus_tag="PKK20_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99425.1"
                     /translation="MKNALSPFVCSLATVVAAHGAVIDSGLLGGGLIPDNGGALFRWT
                     YAGDSARIAGLTLSVAISGSGGGGAFNGDLYATLRHSSAATTGFAVLLNRPGRADAGA
                     YGYDDNGITVTFDDSVMAPDIHDYRYALFGDHTVALDRPLSGIWATDGRAVDPNDVAS
                     KSPRTTSLLDFLGMDPRGEWLLYIADVSAGGHAQLDSWRLQINTKAPIPEPAAGALVV
                     ALGLVGCVWMRRSRA"
     gene            complement(6424..>9346)
                     /locus_tag="PKK20_05775"
     CDS             complement(6424..>9346)
                     /locus_tag="PKK20_05775"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU99426.1"
                     /translation="SGEVALRFFGGVMGEATATYTAGPLEGEAEVAAINGSINGGAHI
                     SFEYKDGAMTTEACFEGIYYSAYVKLAGTTYSLFKDAEGKPIEKVYLLPSTCGPDPAG
                     LMNALRTEVIPGVEDAVRAAIARQLGPEAAKQLHAKSGGAPIKFSGRSRATLKSASAI
                     VSGSPGKRGGAQLMDGEGVCAKVKLRLEQEAVLTRNAFNATLEIVNGDTIPLEALEVA
                     VNITDEYYKDMGDVFAIRLPTTPVDGTGEAAARSTWSASWILIPTREAAPESPKLYLV
                     GGTLKYVQGGRQIIVPLQPVPITVYPDPLLYIKYFHQRDVFSDDPFTPQIEPSIPYSL
                     AVLAENRGKGVAKNLRITSAQPQIIENEKGLLIDFEIIASQVEEENLTPSLTVSLGNI
                     EPGATKIGRWLLRSTLQGLFIDYRVTFEHLDAIAGKKTALIDDVSIHEMIHMVAAPAP
                     FADGRPDFLVNDLPDPPMDYPDRVYLSDGRTNEVAVVTAGVADGPPSASDTTVQLTAS
                     LPPGFVYLRVPEPSGGQMKLHGVRQGNRDILMDVHGVTGVANAWVTDRTFVGQGKKPV
                     RENILHLFDYNANAVAGPVTYTLIYEELPPLDISAPISAVKPLPARSALSFAVTWAGQ
                     DADGSAVAAYDIYVSDNGGPFALWLTNTRFTGGQYTGQMGHTYAFYSRAKDAAGNLEP
                     APVSPDAITLVAFETTAPHVSEIADQVMVEDTVAHAVPFAVGDPDTPLDALRINVQSS
                     NLTLLPLSGLAVSGSGADRVLAITPEPGRSGEAQVTLTVSDGSSSASRTFLARVIAVN
                     DAPVAGNDLVTRPLDRGVKVAVSELLANDADPEFDPLTLTGVSAVSRNGANIRLVGGW
                     VFYEPTDSSGNEPDEFTYVVSDGKGGLGEGRVFVQVVSDGPIAAELKLSANPDGTVVL
                     EFVGIPGRWYAIERASSLANPVWEVVDTVQADSIGLIRYTDASPPAGTAFYRAADLSA
                     H"
BASE COUNT         1794 a         3137 c         2800 g         1615 t
ORIGIN      
        1 tgccgaatgt cagcagcagc tccaggatgg ccgcgtccgt gcgcgacgca gcatccccgt
       61 ggatgaaccg ctcgcgcagc cgctcacggt gaccggattt gccattgtcg ccatcgtcca
      121 caacctcgat ataggtttac aaaagacagt ctctcgttgg ttagaagcga tccttatagc
      181 ttgcctaaag ccttcgggca aggagatgct gatcgctgcg taacatgcgt cggggattct
      241 gctcctcgcc gtcaatcaaa acaagcttgg tcttcgggca tctcgccgat ttgtgaaaca
      301 gcgcgaagga cttatggcct ggcgacaagc gaagacatgc gatcgtgtca gccatcaagg
      361 ttctccagcg gcagcggctg tccggacagc cttcaacact ctcgccatct cgctcagcac
      421 cggatcgccg tcaaccttcg ggatcaaaac ggcaagcccg gcactggatc gcctcgccag
      481 tgcctccggg ctcagccgct cggcctcgcg gtaaggccgc aactgattga ccacggtgtc
      541 ggtcttggcc tcgatgctga taacggggaa tccatcttcg taagtggttg attatgaaga
      601 tggagcgagc gaagggattc gaaccctcga catcaacctt ggcaaggttg cgctctacca
      661 gactgagcta cgctcgcttc cagaaaaagc aggagggagt ctagggcttc cgccgcgaaa
      721 tgcaaggatt catttttggg gattccgagg gcagtctgtc gcctttcaag aattcccacc
      781 cgtgggcggg cggctgcgtg ttctggctgc aaacgcaagg cttcgtgaaa tgaccgcgcc
      841 aaagaccggc caccctgccc ggtcttttca tttggaaagt gcttgagtag tcctcgattg
      901 gttgtggagt cccgaatgaa gggtatactt ctgccctctg cgcttgcgct ggagtcgttg
      961 tcggcccact cgtatccgtg cggttgtagc gcccgtacct tgatgctctt tagcggacca
     1021 atggcggatc gaccgacaac acttcgaggc ttttcatcag tcaggccgac cgggctcacc
     1081 gtccagaagc cgcagccagt cgggcggtaa agtgggaggg gcaggcaact cgatgatact
     1141 gtgcaggggt cgggactgtt tggtcgagcc cccggtggcg aggtggaacc agccgtcagt
     1201 cagacccgca tcaatcgtct ccttcgcttc ccattcggct ccggacgcgg tgaagcgggc
     1261 tttggcgagc ggggtccatg cgtcggcgtt ctgtcggacc cagccgcggc cataacgcgc
     1321 gcgccgcact tcgagggcgc tgcggccgtc gcgacggaaa tcctcgacga aggaataata
     1381 gcctctgagc ctttgacctc ctgtgcgggt gcgaaaggtc gcgagcttcc accatgtccg
     1441 cttctccggt tgaaagagcc aggctgtata ggccgttttc tgcccgtcca cttcggcgcg
     1501 caggagaaac cggttggttt cgccaatctt ccacagaaac ggcgccatgc actgcccgcc
     1561 tgtgccctcg ccgccgaagc gtttaatccg cacaccttcg ccttgataga gcacttcgac
     1621 ccgatcctcg ctcttgactg cgccggggtc gtcgccccgg gtcggatccc agaccgagaa
     1681 gatcgccacc ttgtcctccg gcttccagag ctgctggagg ccgaaatagc cggtgttcca
     1741 tccgcacgcc atgaagtaac tcccattgac ggattcatcg attatcatct cggtgtagaa
     1801 caggtcgccg tccggcgccg gatagccgag gtgaacggac cgcgcagcgc ggtaggcggg
     1861 agcgtcggcc gcccagaggc acgtcggcat cagaacagca atgagcccga tgagaactcg
     1921 catgggtgca atcgtgccct cgaattacac cgtccgcaac cttgctgatg ggaccgtggg
     1981 aaggacgaca acgggtgctt gggatggaga ctggagggac gatttccgcg ctaatccttg
     2041 ggcttgaaca cgggcagggc cagtttccac tggataccgg cgaggcgcgc caggagaatt
     2101 gcgagaaccg aaatgaccat gttcgtcaag tgagcgacgc gcgcggattg cagcaccacg
     2161 tacagcaccg cgccaaagaa tgcggccgtg gcgtacaggt agatgtccgg tcgaaagacc
     2221 aggggaatct cgttcagcaa gaggtcgcgc agaatgccgc cggcaactcc cgtggtcacc
     2281 ccgagcatga ttgcgatagg tgccggcgcg tggaaggcgc aggctttcac cagaccaacg
     2341 atcgtgaaga gcgccaacgc gaacgcatcc gcaacctgca aggaacggag atgaatctcg
     2401 tgcgtgcggg ccaggaaaaa ggtcaccacc gccgctccgg ccgcggtgta cacgtaatgg
     2461 gggtccttgg cccagaaaac cggatgatcg ccgagcacta agtcccggat ggtgccgccc
     2521 ccgaaggccg tgaccagcgc cagaacaatc acgccgaaaa gatccacgtg cttgccgcgc
     2581 gcggccatga cgccggtgac cgctgccaca cccacgccaa actgctccag aatgtaacgg
     2641 ctcgtcatgc gcaagaggca ttatcacagc ccccgaaata atctatggaa attctcctcg
     2701 acccgggcgg ccagactctg gatcggctca cccagcatcc cggcaacgta atcgtaaaca
     2761 accaccaggt tggcggggtg attcagcgga cgccccgagg tgcgggccgc gtcggccgga
     2821 aagtcgccgg aacagaccgc ccctgcctcg atgcacggac cgtgcaaccg aaactgcgcc
     2881 agggcagacg gcaaacactg gtccggagcg tctgtttcaa tcagcaaccg atccggcggc
     2941 acggcccgaa acacctcgcg ctggcgctct ttgcccggcc gcgcgaagta cccgggaaag
     3001 ctgaaatagg cacccaaccg cgccagcggc ttcaccattt caagaggccc tccgtagctg
     3061 tgcagaagaa aaccgcaagc cggacgcggg tttgccgcca gcaactgttc gaggcgcccc
     3121 caggctcgca ggcaatgaat gctcgccgga atggcgcgtg cggccgccag ccgcaattgc
     3181 gccagaaaca cctcctcctg actctcgtag gacagacccg gcttccatcg gtcgagcccg
     3241 atctccccca ccccggaggc gatctcatcg aggaaccgcg ccagattcgc ctcccaatcc
     3301 gccgcgcgat cgcccaggtg ccacggatga tagccgaaac tgggaatgac ttgcgggtgc
     3361 tcccgcgcca gggccagcac ctgcggccag tcggcggggc aggagccgtt caccaccatg
     3421 cgccgaaccc cggccttcac gcaggcggcg atcagattcg attgccgtcc gccaaagcgc
     3481 tcgtcctgca ggtgattgtg agcgtcgtag taattcactg gcctggcgcc gtcagcacaa
     3541 atgcaccgta gccgaccacc cgggaacgcg aaccggccgt gtcgcccgag ttgcgcaggt
     3601 caatggcccg gcactcgagt ccgcgatccc gcgccacttc gagcaacccg cggacgcctt
     3661 ggaaaccgca ggcgtcatac ccgtcgagcc tctcgccctc aaatgcctcg atcgcacggg
     3721 cggtggcctc atcgaactgt ctggccgtat tgtagttgtg gtagtggctc aaatccgtgc
     3781 tcgccacgat gcacgactcg ggcccgcccc aaagaacctc gatcagacgg gccatttccg
     3841 actgcgtcgc ctcccccacc agcagcggca ccagttcgaa ctcccccaac accacctgaa
     3901 gaaacgggat gtgcacctcc agactgtgct cctcccgatg cgggtcttcg gatatcgcaa
     3961 cccccggcag accggccgca cacgcgattc cggcccgatc cagaggcact ctccccagcg
     4021 gggtctcaaa ggcatccatc ccgctcgcag ccaccccatg aaaacgggcg aagtgcgacg
     4081 ggccaaccag cacaacacgc cgaatctggt cgcgtccgga ctccagacgc gcatacgctg
     4141 agcccgcaat cggccccgag tacatgtacc cagcatgcgg tgcaatcagg gccttcggga
     4201 ccggcccggt cgacctgggc gcggcggcga ggtaatcgct gacctccttg cgcagttgcg
     4261 ccggattcgc cgggtagaat cgccctgctt gcgccggagg gcgcacgcgc ccgcattccg
     4321 atctcgagtc tgctttcatg tcgacaacgc gtttcgccga tcaagcattc ctgactctcg
     4381 ccgcgattca cccgccgctg cccagatgcc ggggatcgat cgcccgcagc cagggcaaca
     4441 tcctcgaggg ctcagcctgt cctcgatcat gcgaaaaccg cgacgccgga ccagtgtctg
     4501 cccgcagcct gaacaccgcg tgtcttccca gtggccaacg cgtccggaca ggttgcccgc
     4561 gtacacgaaa cgaagacctt cggcgactcc catctccgcc gcgcgcacaa gatcgcccgc
     4621 ctccgtagcg cgcggttcct tcatgcgata gtccggatga aacgccgtca cgtgccacgg
     4681 gatgtccggg ctcacggaaa ccaggaaacg cgccatcgcc ctcagttcag cctccgtgtc
     4741 gttgaagccg ggcaccagga gcgtcaccac ctcgacccag attccacggg cgtgcgcgtc
     4801 gcgaatcgtt ccgatcacgc gctcgagcgt gccgcccaaa gaccggtagc ggcggtcgtc
     4861 aaacgtcttc agatcgatgt tcaccgcttg cagccaaggc tcaagaaaat cacgcgcctc
     4921 tcgggtggca ttgccgttcg agacgaaacc gcacaccagc cccgccggac gcgcgcgcgc
     4981 gaagacctcg gccgcccact cggacgtgat gaacggctcg ttgtagctcg acaccatcaa
     5041 ccgcgccccc tgtcgttgcg cagcgcccac caactcatcg gatgtgattg gctggatgcg
     5101 cgtcccggcc gactcgtctc gcaaggcctg actcgtgatc cagttctggc aataggcgca
     5161 atgaaagtca cagccaaaca tcccgaacgt gagcgcgccg ctgccgggaa gcagatgata
     5221 aaacggcttt ttctcgaccg gatcgcacgc cgcgcccgaa acgtatccga acggcacacg
     5281 caactgaccg gcgctgttca accgcacctt gcacacgccc cgacgtccca agccaatcag
     5341 acagcgatgt ccgcaggcaa aacagcggac cttcccgccc tcggcacgcc agagagaccc
     5401 aactgccgtg tgccggtcca gcgattccgc gaatcggctc gatgcaggga cccatgactg
     5461 accaggctcg tcccgtggga ttggcatggc caaagaatac tccccgcgcg gcatccgggc
     5521 aagatcgaga gccttcacca gcaatgtgaa gagtcacact cgtgactggc tggtccgcgg
     5581 cggagcctcg ctgatccgca cgcttcggac gccgtccgcg caaagggcag accgagcccg
     5641 caagagcgtt gcggggcggg ttgaaaatgc tccaactcca caccccacgc cgctcatgat
     5701 tacgcgcggc tccgtcgcat ccagacgcac ccgaccaggc ccagagcgac aacgagagcg
     5761 ccggcggccg gttcggggat aggggccttg gtgttgatct ggaggcgcca cgagtccaat
     5821 tgcgcgtggc cgccagccga aacatccgca atgtaaagca gccattcgcc tctcgggtcc
     5881 atgcccagga agtccagcag cgatgtggtc ctcggactct tgctcgcaac atcattcgga
     5941 tcgaccgcgc gtccatcggt cgcccatatt cccgaaaggg gcctgtcgag cgcgacagtg
     6001 tggtcgccga acagcgcgta gcggtagtca tgaatgtctg gcgccatcac actgtcgtcg
     6061 aacgtcacag tgatcccgtt gtcgtcgtag ccatacgctc cggcatcggc gcgccccggc
     6121 cgattcaaca gcacggcaaa gccggtcgtg gctgcgctcg aatggcggag cgtggcatac
     6181 aaatcgccgt tgaaggcgcc gccgcccccc gaaccgctga tcgccacgct cagtgtcaag
     6241 ccggcgatcc gcgccgaatc gcccgcgtag gtccatcgga acagcgcgcc gccgttgtcc
     6301 gggatgagac cgccgcccaa taaacccgag tcgatcacgg cgccgtgagc ggcaacgaca
     6361 gtcgccagac tgcaaacaaa cggagacagt gcattcttca taggtcagga atcgctttca
     6421 ggtttagtgt gcggacaaat cggcagcccg atagaacgcc gtgcccgcgg gcggactcgc
     6481 gtcggtgtag cggatcaggc cgatcgagtc ggcttggacc gtgtcgacga cttcccacac
     6541 tggattggcg aggctcgagg cgcgttcgat ggcgtaccag cgacccggga tacccacgaa
     6601 ctcgagaaca acggtcccgt cgggattggc ggagagcttc agttccgcgg cgatcgggcc
     6661 gtcggacacc acctgcacaa acacccggcc ttcacccagc cctcccttgc catccgagac
     6721 aacgtatgtg aactcgtcgg gctcattgcc gctcgagtcc gtcggttcgt aaaacaccca
     6781 gccgccaaca agccggatgt tcgccccgtt ccgcgacacg gccgagacgc ccgtgagggt
     6841 gagcgggtcg aattcgggat cggcgtcgtt ggccagcaat tcgctgacgg cgaccttgac
     6901 gcctcgatcc aaaggccgcg tcaccaggtc gttgcctgcc actggcgcgt cattcaccgc
     6961 tatcacccgt gccaggaatg tgcggctcgc actcgaggac ccgtcgctca ctgtcagcgt
     7021 cacctgcgcc tcgccggacc gtccgggctc gggcgtgatc gccagcaccc ggtcggcgcc
     7081 gctcccactc accgccagtc cggacagcgg cagcaatgtg aggtttgagg actggacatt
     7141 gattctgagc gcgtccaacg gcgtgtccgg atcgccgacc gcgaacggca cggcatgggc
     7201 caccgtatct tcgaccatga cctggtccgc aatctccgac acgtgcggcg cagtggtctc
     7261 gaaagcgacc aaggtgattg catccgggct gaccggagcg ggctcgaggt tgcccgccgc
     7321 gtccttggcc cggctgtaga aggcgtaggt atggcccatc tgccctgtgt actgtccgcc
     7381 cgtaaaccgc gtgttcgtca gccagagcgc gaacggccct ccattgtcgg acacgtagat
     7441 atcgtaggcc gccacggccg agccgtcggc gtcctggccg gcccaggtga cggcgaacga
     7501 tagggcgctc cgagccggca gtggcttcac ggcgctgatc ggcgcgctga tgtccagcgg
     7561 cggcagttcc tcgtagatga gggtgtaagt caccggccct gcaacggcgt tggcgttgta
     7621 gtcgaacagg tgcagtatgt tctcgcgcac cggtttcttg ccctggccga caaacgtccg
     7681 atcagtgacc caggcgttgg ccacgcccgt caccccatgc acatccatca ggatgtcccg
     7741 attcccctgc ctgacgccgt ggagcttcat ttggccaccg gacggctcgg gcacgcgcag
     7801 atacacgaat ccaggcggca ggctcgccgt caactgcacg gtggtgtcgg atgcgctggg
     7861 cggtccgtcc gcaacaccgg ccgtgaccac ggccacctcg ttggtccgcc cgtcgctcag
     7921 gtacacgcga tccgggtaat ccatcggcgg atcgggcaga tcgttgacta ggaaatccgg
     7981 gcggccgtca gcgaacgggg cgggggcggc caccatgtgg atcatctcgt gaatgctgac
     8041 gtcgtcgatg agggccgtct tcttgccggc gatcgcatcg aggtgctcga aggtgacgcg
     8101 atagtcaatg aacaggccct gaagcgtcga gcgcagcagc caccgcccaa tcttggttgc
     8161 gcccggctcg atgttgccca aactcacggt cagtgacggc gtgaggttct cctcctcgac
     8221 ttgcgaggcg ataatctcga agtcgatcag gagccccttc tcgttctcga tgatctgggg
     8281 ctgcgccgag gtgatgcgga ggttcttggc cacacctttg ccccggtttt cggccagcac
     8341 cgccagcgaa tacggaatcg acggctcgat ttgcggtgtg aaggggtcat ccgagaacac
     8401 gtcacgctgg tggaaatact tgatgtacaa cagcgggtcc ggatacaccg tgatcggcac
     8461 cggctgcaac ggcacaatga tctgacgtcc gccctgcacg tacttcaacg tgccccccac
     8521 cagatataac ttcggcgact ccggcgcggc ctcccgcgtc ggaatcagaa tccaggacgc
     8581 gctccatgtg ctcctggccg cggcctcgcc cgtcccatcc accggcgtgg tcggcagccg
     8641 gatcgcaaac acatccccca tgtccttgta gtattcatcg gtgatgttca ccgcaacctc
     8701 gagcgcttcc agcgggatcg tgtcgccgtt gacgatctcg agcgtcgcgt tgaacgcgtt
     8761 gcgcgtcaga accgcttcct gttcgagacg cagcttcacc ttcgcgcaaa cgccttcgcc
     8821 atccatcaac tgcgcgccgc cccgcttgcc cgggctcccg ctgacgatcg ccgaggccga
     8881 cttgagggtc gcacgcgagc ggcccgagaa tttgatcggc gcgccgccgg acttggcgtg
     8941 gagctgcttg gcggcctcgg gtcccaactg ccgggcaatc gccgcgcgaa ccgcgtcttc
     9001 aacgccgggg atcacctcgg tccgcaacgc attcatcaga ccggcagggt ccggtccgca
     9061 cgtcgacggc agcaaataga ccttttcgat cggcttgcct tcggcgtctt tgaacagcga
     9121 gtaggtcgtg cccgccagct tcacataggc cgaatagtag atgccctcga aacaggcctc
     9181 ggtcgtcatc gccccgtctt tgtactcgaa tgagatgtgg gcgccgccat tgatgctccc
     9241 gttgatggcc gccacttccg cctccccctc gagcggcccg gccgtgtacg tggccgtcgc
     9301 ttcgcccatg acgccaccga agaaccgcag cgcgacttcg ccgctg
//
LOCUS       DAOQRO010000110         9329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6797_length_9329_cov_5.239163, whole genome shotgun sequence.
ACCESSION   DAOQRO010000110 DAOQRO010000000
VERSION     DAOQRO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_9329_cov_5.239163"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..3319
                     /locus_tag="PKK20_05780"
     CDS             413..3319
                     /locus_tag="PKK20_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNU99427.1"
                     /translation="MRNTKTLWRLFASALLAAATSVSAVSQGFIANPSFENNFTQPWP
                     FYGTIDNWNSTGGGSGVNENAGPFHNSGTPVPDGLRVGFHQGAGTLSQDIAGLTAGKQ
                     YWIQFRYDARSGSDLDLAVRFSTINWGGAMDETLATMLKVKPATLTGSPYYSCTVPFT
                     PDFEMGTLTFDITTRGDSTVLLDAVTIVQRDAGNFTVMNPSFEASGAVYDGAPSVGAD
                     WPAISGWVKTGVAGVDDGTGGRADNGAIPDQALVAFIADEGSLTQTLRPLVPGNDYQL
                     AFAYNATSGATAHLQVSVDGTVIWEKDVSAVGGGADYARQTVTFKAAASTAQIGFANT
                     AAGATVLLDDVKVLGETGSAMPPMDMTPAKIALRIGEEGTATITVPPERLAEGPAVVK
                     VRSGNTTVFVLPDADASGTVSLAFEGATTTQSFKIRGVAVGNAVVEITDPDGTPFPAD
                     ITAVFVAGTTFVLNPSFEVDKDNGVGTAAVSCWTTGGANIGMAGTGNPFLSAADLTIP
                     DRRQVLRIQGGGGTVSQMIKGLKVGQLYGLQFFYNARTVENPYELDLQVSFAGQQLAN
                     IEGIIPAGQLGLTDYYFKELRFTAAAESGLLEFKTVVASGDATLFLDGVSIIPRLADE
                     IAVKNSSFEASIMGANWPGYFHPERVAGWVPSGGYGVNGYSPTTFWIEPFFDNGINSD
                     QDSVYFNQNAGNIRQTVEGLTPGQEYTLIFDYNFRDGRATGSSNAPNLGQVEVSVDGG
                     VLFTSEELPPIDTVSPWTGTRHTMPFYQAFVPFTPWMDTAEVQIAHIGTFPDKTLLLD
                     NVHVVPGTYTPPSITKELVDQAVQAGTPVTFSTAATGNTLAYRWYRNGIRLADGNGIS
                     GASTATLSLASPGEDDAGTYTVIVTDGLGVAGSVAALAVEPAPTDVTLTISLLAASNI
                     RIAWPATASAFRLQSAATVNGVYADDPAPVMIENDLCVVTVAAQDAAKFYRLTN"
     gene            3461..3997
                     /gene="lepB"
                     /locus_tag="PKK20_05785"
     CDS             3461..3997
                     /gene="lepB"
                     /locus_tag="PKK20_05785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNU99428.1"
                     /translation="MIIAMRDPASGPVASVPLWQRMIVGKNPKVTLVRALLLGLGCWL
                     VFRHVLLPVRVNGISMEPTLRNGSVNLVNRIPYLWRAPRRGEIVAIRTTGTSIMYLKR
                     IIALPGESVQMQGGRVWIDGRLLDEPYLGEAEPWEIGRRTLGPEDYFVMGDNRTMPKE
                     LHWGGVIKRSRIVGSTLW"
     gene            4005..4496
                     /locus_tag="PKK20_05790"
     CDS             4005..4496
                     /locus_tag="PKK20_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99429.1"
                     /translation="MKWGKLLLVLALGGAAFAGLRGCLPSDQKRILRLLTSLAQDVSA
                     PAQGRTLADLAAANRIADHFAPGFQIQVTVPGAPDVAVSDRAELIQTIAAAKGRTRQV
                     EVRFLDPQIVELGPASAVIEATAQARVSGDPELYVVEIRFTLVKLEGLWRVRQVENVP
                     TFE"
     gene            complement(4742..6211)
                     /gene="gnd"
                     /locus_tag="PKK20_05795"
     CDS             complement(4742..6211)
                     /gene="gnd"
                     /locus_tag="PKK20_05795"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNU99430.1"
                     /translation="MEPQADIAVIGLAVMGQNLILNMNDHGFTVVAYNRTVSKVDEFL
                     AQEARETKIVGAHSLAEMAALLKRPRRVMLMIKAGRAVDELIDQLLTVLEPGDIIIDG
                     GNSLFEDTIRRTKFVESKGLLYIGTGVSGGEEGARHGPSIMPGGSPSAWPHVKPIFQA
                     IAAKVVDAAGGPEQACCDWVGEGGAGHYVKMTHNGIEYGDMQLICEAYHIMKDGLGMT
                     AGEMHHVFKEWNTGELESYLIEITRDILAKTDDDGTPLVDKILDTAGQKGTGKWTVIS
                     SQDLGIPITLIAEAVYSRCVSALKDQRVLASKHFKQGNPKFAGDRVAWLGDIRKALYA
                     SKIISYTQGYMLMRAAAQAHHWNLNYGGIALMWRGGCIIRSVFLGEIKKAFDKNPDLA
                     NLLLDPFFKKAIKGCVRSWRKVVSTAVKKGIPVPALSTALAFFDGFRSARLPANLLQA
                     QRDFFGAHTYERVDQPRGQFFHTNWTGAGGRVSSSTYSV"
     gene            complement(6228..7052)
                     /locus_tag="PKK20_05800"
     CDS             complement(6228..7052)
                     /locus_tag="PKK20_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNU99431.1"
                     /translation="MSKIYFGINMEFVRHADQSFEWGVEKAAELGYTHIEPMVHWGRE
                     LLSEAGYFHSVSMLDDPLRIKRACEQAGVKLSGLSAHTPITKPEIGVEYLKQAIRFAA
                     ECGAPVVNTDEGRKQPWTTEEEDFTLMRYTLMEAARVAEPRGILIGLECHQQYSKTPD
                     GLDRIHKLVKSPAIGINFDTGNAFLCGHDPYKWLKRVVKRLVHLHAKDISVQQGSAER
                     GKVTGTAVGCACGEGVIDWTRVIRLCREAKRDIVLSVECGTVDQAERSIQHLKSLI"
     gene            7448..8629
                     /locus_tag="PKK20_05805"
     CDS             7448..8629
                     /locus_tag="PKK20_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNU99432.1"
                     /translation="MLTRRAFLERMAGAATAVAGTGMAWASGAPEAEGPVRRAPTSIG
                     LCKRYEYPEVRRALGRMLDEIGGVRRLVRRRHVVVKLNLVNTSVEDVGGVPLWLTVTT
                     HPVVAMALGSLLVESGARLVTFCDQLPFRALDEEAFAGYGYDAKAFNQALNGRARFEN
                     TRNRGRHRQYAWVRVPDGELASAWEVNRTYVETDVLVSVGKLKSHISAGVTGGMKNLF
                     GVPPSSLYSEDLKDEPDEDAVGYRSATLHSCVRRPFTSAESVTGRSVEGDHGYNVPRF
                     IVDLAAAFPIDLVVIDGISTLQTAEGWWIGSMVSVTRPGLLIAGRNPVCTDAVAAAVM
                     GFNPDAPDRTFPFINGANHLALARRRGLGENRLDRLEIAGVGLEAARFEFHPTYQRVQ
                     G"
     gene            8688..>9329
                     /locus_tag="PKK20_05810"
     CDS             8688..>9329
                     /locus_tag="PKK20_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU99433.1"
                     /translation="MTNRFADGSEVWSAAGGGPGRQGLWDGRVLIESTPVWCLAVKGA
                     VQASVVFDAAGRSLVADMAGWVHAFSVEGRLLWGRQIEGAVSATPAVDTTRDRVFAGT
                     HLGWVYAMRASDGAVLWRQRLPTRSDARIGSDLLLTPGAAEVVSSSWGGRFCALDAGT
                     GQTRRTWDAGLSPQAGASADRRGNLYSMRAVRGEGVVCVRLDRVEVPPPIGAGP"
BASE COUNT         1640 a         2930 c         3052 g         1707 t
ORIGIN      
        1 caccaccact gcccttgccg cagacacatg tacgccaaaa cagaaggcgt gtcaacaatt
       61 agaaacctgt cacttcatct ttctctcaat ccccccacat agatatagat aagtacgaac
      121 ctgtcacttt atccgcgcca agcgccggct gtggcccagg aaagagatga ccaccaatca
      181 cacgattcat gtttcccctc gaattcgtgt cattcatgat tcgaggttcg cctctcgatg
      241 tcgcgtgaag acatccctgg gttgcctgat tttgcctgat ttgccttgaa cggccttcag
      301 ggatcggaca tggttcacat cgtcggaaag gcaatgcttg acaatcgtac cagtaccaag
      361 ttgcgccctg gcagaagccc ttaaccaagg aaccaagaag gcagcaaaca ccatgcgaaa
      421 caccaaaaca ctttggcgcc tgttcgcgag cgccttgctg gctgccgcga cgagcgtgtc
      481 ggccgtgagc caggggttca tcgcgaaccc cagcttcgag aacaacttca cccagccctg
      541 gcccttctat gggaccatcg acaactggaa cagcacaggg ggcgggtcgg gcgtgaacga
      601 gaacgccggt ccgtttcaca acagcggcac acccgtcccg gacggcctgc gggtcggctt
      661 tcatcaggga gccggcaccc tgtcccagga catcgccgga ctgactgcag ggaaacaata
      721 ctggatccag ttccgctacg atgcgcggag cggcagcgac cttgatctgg cggtcaggtt
      781 ctcgacgatc aactggggtg gagcgatgga cgagaccctc gccaccatgt tgaaagtcaa
      841 gccggccaca ctgaccggct cgccctatta ctcctgcacc gtgccgttta cccccgattt
      901 cgagatgggc acgctcacct tcgacatcac cacccgcggt gattcgaccg ttttgttgga
      961 tgccgtgaca atcgtccagc gtgacgcggg caatttcacg gtgatgaacc ccagtttcga
     1021 ggccagcggc gcggtctacg atggcgctcc cagcgtcggc gccgattggc cggcgatcag
     1081 cggttgggtc aagaccggcg tcgccggcgt ggatgacggc accggcggac gggcggacaa
     1141 cggcgccatt cccgaccagg ccctggtggc cttcatcgcc gacgagggat ctctcacaca
     1201 gaccctcagg cccctggtgc caggaaacga ttatcagctt gcgttcgcat acaacgccac
     1261 ctcgggtgcc acggctcacc tccaggtgag cgtcgacggc acggtcatct gggaaaagga
     1321 tgtgtccgcg gtgggaggcg gggcggacta cgcccggcag accgttacgt tcaaggcggc
     1381 ggcaagcacc gcccagatcg gctttgccaa cacggcggcc ggcgccacgg tgctcctgga
     1441 cgacgtcaag gtcttgggcg aaacaggatc ggctatgcct ccgatggaca tgaccccggc
     1501 gaagatagct ttgcgcatcg gggaagaagg cacggcaaca atcacggttc cgcctgagcg
     1561 cctggccgag ggacctgccg tcgtcaaggt gcgcagcgga aacacaaccg tcttcgtctt
     1621 gcccgacgcg gacgccagcg ggacggttag cctggcattc gaaggcgcca caaccaccca
     1681 gtccttcaag atccgaggcg tcgccgtcgg caacgccgtc gtcgagatca ccgatccgga
     1741 cggaacgcca ttcccggcgg acatcaccgc cgtgttcgtg gccgggacga ccttcgtcct
     1801 aaaccccagc ttcgaagtcg acaaggacaa tggcgtcggc acggccgcgg tctcctgctg
     1861 gaccaccggc ggcgccaaca ttggcatggc ggggacgggc aatcccttcc tgtccgccgc
     1921 ggacctgacc atcccggatc ggcgtcaggt gctgcgaatc cagggcgggg gcgggacggt
     1981 atcccaaatg atcaagggtc tcaaggtcgg tcagttgtac ggcctgcagt tcttctacaa
     2041 tgcccggacc gtcgagaatc cgtacgagtt ggacctgcag gtcagcttcg ccggccagca
     2101 actggccaac atcgagggca tcatccccgc gggacagttg gggctcactg actattactt
     2161 caaggaactg cgcttcaccg cggcggctga gagcggtttg ctcgaattca agaccgtcgt
     2221 cgcatcgggc gatgcgacgc tgtttcttga cggggtatcg attattccgc gattggccga
     2281 cgagatcgca gtcaagaatt cgagcttcga ggccagcatc atgggggcca actggcccgg
     2341 ttacttccac cccgagcggg tggccggctg ggtcccgtcg ggcggctatg gcgtcaacgg
     2401 ttacagcccg acaacgttct ggatcgagcc gttcttcgac aacggcatca actccgatca
     2461 ggacagcgtc tacttcaacc aaaacgcagg caacatccgc cagaccgtcg agggcctgac
     2521 accaggccag gaatacaccc tgatcttcga ctacaacttc cgcgatggcc gcgccacagg
     2581 ctcctccaac gcccccaacc tcggacaggt cgaggtgagc gtcgatggcg gcgtcctctt
     2641 cacgagtgaa gagcttccgc ccattgatac cgtctcgccc tggaccggaa cccggcacac
     2701 catgcccttc tatcaggcgt tcgttccgtt cacgccgtgg atggacaccg ccgaagtcca
     2761 gatcgcccac atcggcacct tccctgacaa gaccctgttg cttgacaacg tgcatgtcgt
     2821 gcccggcacc tacacgccgc cgtccattac gaaggagctc gtggaccagg cggtccaggc
     2881 cggcacgcca gtcaccttca gcaccgcagc gaccggcaac actcttgcct atcgctggta
     2941 tcgaaacggc atccggctcg ccgacggcaa cggcatcagc ggcgccagca ccgccacact
     3001 gtctctcgcc agtccaggcg aggatgacgc cggcacgtac actgtcatcg tcaccgacgg
     3061 cctgggtgtg gccggcagcg tcgccgcgct ggccgtcgaa ccggccccga ccgatgtcac
     3121 gctcacgatc agcctgctgg cggcaagcaa catccgcatc gcctggccgg cgaccgcttc
     3181 cgcgttccgt ctgcagtccg ctgcgacggt caacggcgtc tatgccgacg atcccgcgcc
     3241 ggtgatgatc gaaaacgacc tctgcgtcgt gacggtggca gctcaagacg ccgccaagtt
     3301 ctatcgcctg acgaactgat cgctcgattc gacggttcaa ctcgcactgg gcgccggcgc
     3361 acgccgccgg cgccccttct gttctatcgc cacgacgttc agctctcgat gcggggcggg
     3421 gccatactct ggcgctctgg cttcgcgaac ggactctggt atgatcatcg ccatgcgcga
     3481 tccggcgtcc ggtccagtcg cttccgtccc cttgtggcag cggatgatcg tgggaaagaa
     3541 ccccaaggtc accctggtgc gtgccctgct cctgggcttg ggatgctggc tggtcttccg
     3601 gcacgtgctc ctcccggtcc gggtcaatgg gatcagcatg gaacctaccc tgcgcaacgg
     3661 ctcggtcaac ctcgtcaacc ggattccgta cttgtggcgc gcgccgcgcc gcggcgagat
     3721 cgtcgcgatt cggacgaccg gcacgagcat catgtatttg aaacgcatca tcgccttgcc
     3781 gggcgagtcg gtccagatgc agggtggacg ggtgtggatc gacggaaggt tgctcgatga
     3841 gccctatctt ggggaggcgg aaccttggga aatcgggcgg cgcactctgg gtcccgagga
     3901 ttacttcgtg atgggggaca atcgcacgat gccgaaagaa ctgcattggg gcggcgtgat
     3961 caaaaggagc cgcatcgtcg gcagcaccct gtggtgacca ggccatgaaa tggggcaagc
     4021 ttttgctggt gctggcgctg ggcggcgccg cgttcgccgg cctgcgcggc tgcctgccat
     4081 ccgaccagaa gcggattctc cggcttctga caagtctggc tcaggacgtg agcgcccccg
     4141 cgcagggcag gactctcgcg gacctcgcgg cggccaatag gatcgccgac cacttcgcac
     4201 ccggatttca aatccaggtg accgtcccgg gcgccccgga cgtcgccgtc agcgatcgcg
     4261 cggaactgat tcagacaatc gcggcagcga aaggccggac acgccaggtg gaggtccgat
     4321 ttctggatcc tcagatcgtc gaacttggac cggcctcggc ggtgatcgaa gcaacggcgc
     4381 aggcgcgggt gtcgggtgat ccggaactgt acgtggtcga gattcgcttc accttggtga
     4441 aattggaagg tctctggcgc gttcgtcagg tcgagaatgt cccgacattc gaatgacacc
     4501 aggatgcgag gccgtgggac tctctggggc gccgcggccg ggtttgagcc gcgcttcgcg
     4561 ctattggggc cgcgctccgc gcttgccatc ctcgacgcgc cggccagact gcccttgcat
     4621 gtgcagcaat cacccgaccg gcaacgcacg gtgacggggc gggagaggcg gctggggtcg
     4681 gaagactcga cagcggggca gggccgggct tgtcaatcca cccggccctg ccgaagaacg
     4741 gctatacgga gtaggtgctg ctcgaaacgc gcccgccggc gccggtccag ttggtgtgga
     4801 agaactggcc gcgcggctga tcgacccgct cgtaggtatg ggcgccgaag aaatcgcgct
     4861 gggcctgcag caaattggct ggcagccgcg cgctgcgaaa gccgtcgaag aacgccagcg
     4921 cggtgctgag agccggcacg gggatgccct tcttgacggc ggtgctgacg accttgcgcc
     4981 aactgcggac gcagcctttg atggccttct tgaagaacgg atcgagcagg agattcgcca
     5041 agtccgggtt cttgtcaaag gcctttttga tctcgccgag gaaaacgctg cggatgatgc
     5101 atccgccgcg ccacatgagg gcgatgccgc cgtagttgag gttccaatga tgcgcctggg
     5161 cggcggcgcg catcagcatg taaccctggg tgtagctgat gatcttggat gcatagagcg
     5221 ccttgcggat gtcgcccagc cacgcgacgc ggtcgcccgc gaactttgga ttcccctgtt
     5281 tgaaatgctt ggacgccagc acccgctggt ccttgagagc cgacacgcag cgggaataga
     5341 ccgcctcggc aatcagcgtg atcggaatac cgagatcctg agacgagatc accgtccatt
     5401 tgccggtgcc tttctggccc gcggtatcga gaatcttgtc caccagcggc gtgccgtcgt
     5461 cgtccgtctt ggcgaggatg tcgcgggtga tttcgatgag gtaggactcc aattcgcccg
     5521 tgttccactc cttgaacaca tgatgcatct cgccggccgt catgcccagg ccatccttca
     5581 tgatatgata ggcctcgcag atgagctgca tgtcgccgta ctcgatgccg ttgtgggtca
     5641 tcttgacgta gtggccggcc ccgccctcgc ccacccagtc gcaacaggcc tgctcgggcc
     5701 cgcccgcggc atcgacgacc ttggccgcaa tggcctggaa gatcggcttc acatgcggcc
     5761 atgccgacgg gcttccgccc ggcatgatgc tcgggccgtg gcgggcccct tcctcaccgc
     5821 cggacacacc ggtgccgatg tacaggaggc ctttggactc gacgaatttg gtgcggcgga
     5881 tggtgtcctc gaagagcgag ttcccaccat cgatgatgat atcccccggc tcgagcacgg
     5941 tcaggagttg gtcgatcagt tcgtcgaccg ctcgtccggc cttgatcatc agcatgaccc
     6001 ggcgcgggcg cttgaggagg gcggccatct cggcaagcga gtgggcgccc acgatcttcg
     6061 tctccctcgc ctcctgcgcc aggaactcgt cgaccttcga cacggtccgg ttgtaggcca
     6121 cgaccgtgaa gccgtggtcg ttcatgttca gaatcaggtt ctggcccatg acggccagtc
     6181 cgataacggc aatgtcagct tgtggttcca taatcagtct gcggttctca gatgaggctc
     6241 ttcaaatgtt gaatggatcg ttcggcctga tcgaccgttc cgcactcgac cgaaagcaca
     6301 atatcgcgtt tggcttcgcg gcacagccgg atcacccggg tccagtcgat cacgccctct
     6361 ccgcaggcgc agccgacggc ggtgccggtg accttgccgc gctcggccga tccctgctgc
     6421 acagagatgt ccttcgcgtg caggtggacc aggcgcttaa cgacccgctt cagccacttg
     6481 tagggatcgt gtccgcacaa gaaggcgttg ccggtgtcga agttgatgcc aatcgcgggg
     6541 cttttcacca gcttgtggat gcggtccagg ccatccgggg tcttgctgta ctgctggtgg
     6601 cactcgagcc cgatcaggat gccgcgcggt tcggccaccc tggctgcttc catgagcgtg
     6661 tagcgcatca gcgtgaaatc ctcctcctcg gtcgtccagg gctgcttccg gccctcgtcg
     6721 gtgttgacta cgggcgcgcc gcattcggcg gcgaagcgga tggcctgttt gagatactcg
     6781 accccgatct cgggcttggt gatcggggtg tgagcggaca gtccggagag tttgactccc
     6841 gcctgttcgc acgcccgctt gatccgcagc gggtcatcca gcatcgagac gctgtggaag
     6901 tagccggcct cgctcaggag ctcgcggccc cagtggacca tcggctcgat atgggtgtat
     6961 ccgagttctg ccgccttctc aaccccccac tcgaacgatt gatcggcgtg ccggacaaac
     7021 tccatgttga tgccaaagta gattttgctc ataggcgatg cgtggatcgt aggtcaccgg
     7081 gagcgggtga cgcaagcgcg attggatcgg agccgcatag gaatggggga ttcatgggga
     7141 aggcgccgag cagagaggcc gcctgggaac aggtccgtga tgaccggcaa ggaccttgat
     7201 cgaggggcga ggcagaccgc cgctcggatt gccgagcagg tgcttgtgca catccggcag
     7261 gccaatgcga agtgatggcg ccgccctacg cccgccgcct cgacaacgca cccgcagtct
     7321 gaaggcgacg cgcctttgac gattcccggc ggcggattat cttaaggggc attcaagacg
     7381 tatgcagggc gaatcagacc actcacgcga tacggctttc catagcgctc cgcattgccc
     7441 cggcggcgtg ctgacgcgac gcgcatttct ggaacgtatg gcgggcgccg cgacggccgt
     7501 ggcgggtacg gggatggcgt gggcgtccgg tgcgccggag gccgaggggc cggtgaggcg
     7561 ggcgccgacc agcattggcc tgtgcaagcg ctacgagtat cccgaagtgc ggcgcgcgct
     7621 cggtcggatg ctggacgaga tcggcggggt gcggcggttg gtgcggcgac ggcatgtggt
     7681 ggtgaagttg aacctggtga acacctcggt cgaggatgtt ggtggcgtgc cgctctggct
     7741 gacggtgaca acgcacccgg tggtggcgat ggccttgggg agcttgttgg tcgagtccgg
     7801 ggcgcggctg gtgacgttct gcgaccaact gccattccgc gcgctcgatg aagaggcgtt
     7861 tgccggctac ggctacgacg ccaaggcgtt caaccaggcg ctgaacgggc gggcgcgttt
     7921 cgagaacacg cgcaaccgcg ggcggcatcg ccagtacgcc tgggtgcggg ttcccgacgg
     7981 cgagttggcg tcggcctggg aagtgaatcg aacctacgtc gagacggatg tgttggtgtc
     8041 ggtcggcaaa ctcaagagcc acatttccgc cggtgtcaca ggcggcatga agaatctgtt
     8101 cggggtgcca ccgagcagtc tgtacagcga agacctcaag gatgagccgg atgaggatgc
     8161 cgtcggctac cggagcgcca cgctgcacag ttgcgtgcgt cggcctttca cctcggcgga
     8221 gtcggtgacc ggccggtccg tcgaggggga tcacggctac aacgtgcccc ggttcatcgt
     8281 ggatttggcg gcggccttcc ccatcgatct cgtcgtgatc gacggaatca gcaccctcca
     8341 gaccgccgaa ggctggtgga ttgggtcgat ggtctcggtg acccggccgg gcctgctcat
     8401 cgccgggcgc aatcccgtct gcacggacgc cgtcgccgcg gccgtgatgg gattcaatcc
     8461 cgacgccccg gatcgaacct ttccgtttat caacggcgcg aaccatctgg cgcttgcgcg
     8521 gcggcggggg ctgggggaga accgcctgga ccgactcgag attgcggggg tgggtctgga
     8581 agccgcgcgc ttcgagtttc acccgaccta ccaacgcgtc cagggatgag tgattcgaac
     8641 ccgggcattg gaatgtgttc attgcgatgt tgcacgagac agttcccatg acgaacaggt
     8701 tcgcagacgg atcggaggtt tggagcgcgg ccgggggcgg gccggggcgg cagggcctgt
     8761 gggacgggcg cgtcctgatc gagtcgactc ccgtgtggtg tctggcggtc aagggcgcag
     8821 tgcaggcgtc ggtggtattc gatgccgccg ggcgatcgct cgtggccgac atggcgggct
     8881 gggtgcacgc cttctcggtc gaggggcggc ttctttgggg caggcagatc gaaggggccg
     8941 tctcggccac gccggcggtg gacacgacgc gggatcgtgt gtttgccggc acgcatctgg
     9001 ggtgggtcta tgccatgcgc gcgtccgacg gcgctgttct ctggcggcag cggctgccga
     9061 ccaggagcga cgcacgcatc gggtcggatc tccttctcac gcccggcgcg gcggaggtcg
     9121 tctcgagcag ttggggcggg cggttctgtg ccttggacgc cggcacgggc cagaccagga
     9181 ggacgtggga cgcggggctt tcgccccaag ccggggccag cgccgatcgg cgggggaacc
     9241 tctactcgat gcgcgccgtt cggggcgagg gtgtggtttg cgtccggctc gatcgagtag
     9301 aggttccccc gccgatcggc gctggcccc
//
LOCUS       DAOQRO010000111         9236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6896_length_9236_cov_4.488727, whole genome shotgun sequence.
ACCESSION   DAOQRO010000111 DAOQRO010000000
VERSION     DAOQRO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_9236_cov_4.488727"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1669
                     /locus_tag="PKK20_05815"
     CDS             65..1669
                     /locus_tag="PKK20_05815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNU99434.1"
                     /translation="MNLSGVSTELRPVVEDLNRRFEGRFNALVTPRLNEVYFHVTMDL
                     VPGFCAHLCRKWKARLVTVFADDARIGHGVFHLYYALALDDAHGLLVLRVPVSPETPQ
                     FVALTNAIPAVNWQEREIQDWFGLRLLGHPNPRRCALHDDWPAVHPLRKDFDLNTVLP
                     PFQGERHKFRPVEGEGVFQVPVGPVHAGIIEPGHFLFSVAGEPVLYLQLRLFYVHKGT
                     EKLFEHLPIPHGVRLAESISGDSSFAHATAFCHAIERTAGAQAPPRARVLRSVCLELE
                     RLYNHIADIGAICTDVAFVTANMHAMRLKEQILRVNEHLTGNRLLRGMACLGGVRFDW
                     DADQIRTLNGLVSDLRPEFDSLVQLVEDSSSTLDRLDTTGILKPEVARDLGVVGIAGR
                     ASGFAYDLRRDFPHAAYDQVQIRVPVYRVGDVRHRLRVRVDEVHESLRLIQTFVTKLP
                     GGPLRIDIGEVPPDRVAFGYVEGWRGEIFHWVRTAPNNRLARCRVKDPSLQNWPALSE
                     AILDNIVPDFPVINKSFNLSYSGTDR"
     gene            1673..2536
                     /gene="nuoB"
                     /locus_tag="PKK20_05820"
     CDS             1673..2536
                     /gene="nuoB"
                     /locus_tag="PKK20_05820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNU99435.1"
                     /translation="MPGILSKSLSTGIVTTRYPDAPAHASPHARGRLEIDWSKWNDAR
                     SAAAVCPTGAIACHDAAQARTVTFDLAKCVFCGLCADTDKAIRMSTVCECAARHRNRL
                     VSTAQYSLNEDGTHRRLIAGPDGATAMRGAVSSLPRNDSIESLGRQIQSRAARLFGRS
                     LHIREVDAGSCNGCEIEIVGLNSPVYDIERFGIHFVASPRHADLLLVTGPVTRNMELA
                     LKKTYDATPEPRLVVAVGACGCSGGIFGVNYATRGPVDSVIPVDVYIPGCPPNPQALL
                     HGILLAIDRGV"
     gene            3041..3454
                     /locus_tag="PKK20_05825"
     CDS             3041..3454
                     /locus_tag="PKK20_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99436.1"
                     /translation="MPGTRLEESVWATVSSSSQAGLQSFLLDFGAAATPTAMGSEPND
                     PAWHWNNITEAIGMSPDGRLPKLATTANLPTDIGFEMLSRFNGADHEGSATSSAFPAN
                     ATRDSVFGNTELFTGLADVFPSFRFVHLTAPRSRR"
     gene            complement(3429..5108)
                     /locus_tag="PKK20_05830"
     CDS             complement(3429..5108)
                     /locus_tag="PKK20_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNU99437.1"
                     /translation="MPPQPVLSPTFKRQCESLRILLQRCLALTEQCGEAGATALLRDR
                     LAHVESAALFVIVGEVKSGKSSFVNALLGEEVCEVAPDPCTMVIQEISYGEQRSRTSL
                     GEQWERIRLPKPSLREITIVDTPGINSVIPHHQTITEQYIPRSDLVIYVLLASNPHRG
                     SEWDLLSRISKEWHRKTVFVLQQADRASQHELDINVEKVKQYARERGVQAPLVFPLSA
                     KRESEEGTDAGFAAFRNYLRQAVESGDVWRMKLEGGRNILRKTVADLLARLRKEQSLL
                     AEDNAFYQDLLGRLEARRRKANSLTRLVVNSLCAVYDRLSSRLEADFSEGLGVGTILR
                     RALPLIRDKDVKTWLKDLHVQFERAAGEEIEAESSRVSKDLSDEMQALLLELREAVAR
                     RQNGQRLQSRPIASARGDILETLGCQLRDLRVADVVSDKEIQGSDLGRLTLAGGGLAA
                     LGTVIALATHLIAFDITGGILAAVGAGLVAVTLLWKRSRILREFSQRLAHSRDDFRDR
                     LDREITLVFDRMFLEIEHDLRDPLTRIESETARVGALIEEAVDLNGATEAL"
     gene            complement(5167..6144)
                     /gene="hflC"
                     /locus_tag="PKK20_05835"
     CDS             complement(5167..6144)
                     /gene="hflC"
                     /locus_tag="PKK20_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNU99438.1"
                     /translation="MKPTVPLLPIVVVVVLAFILLLTAAYTVSETEQVIITQFGRPVG
                     QAVTNAGLHLRMPFIQEVNRIEKRVLRWDGRPSEMPTKDKTYIIVDAFGRWRIGDAKQ
                     YYLRLKDERSALSRLDDILGSEIRNAIARHELIEVIRTTKDREPERDAAIEDARGNVG
                     NLYPIHKGRAKVQQEILANAAPKIEVFGIELMDVQFKRINYNTSVRTRIYERMVSERQ
                     QIAERFRSEGAGEAAKILGQKEKDLQTIESEAYRKTQQIQGDADAEASEIYARAYNQS
                     PEAAALYEFLKTMDTYKAVLTNDTSLILTTDSDFFKFLKRANPLQPTTP"
     gene            complement(6141..7112)
                     /gene="hflK"
                     /locus_tag="PKK20_05840"
     CDS             complement(6141..7112)
                     /gene="hflK"
                     /locus_tag="PKK20_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNU99439.1"
                     /translation="MEIRVGNTYYNIRPRLLLIVAGAALAISLLSTVIYTVQAESQGI
                     VLRFGRYVKTVEPGLHFKLPYGLDQVLEVPVRRQLKQEFGFGAFGSSNPTQVVSADEQ
                     DAERSMVTGDLNAATVEWIIQYRINNPRAFLFNFREPGETLRDVSESVMREVVGDRTV
                     DEVITIGRQEIESEALVRLQAVVKKYGIGVGIDQVQLKNVNPPIPVQPSFNEVNQAQQ
                     EREKSINVANGEYNRAVPRARGEAEQRISAAEGYAVKRINEAQGDATRFNALLAEYLK
                     APEVTRRRIYLETMEEVLPQVRSKIILDDKASQPLPLLQLGAEPKRP"
     gene            complement(7241..8845)
                     /locus_tag="PKK20_05845"
     CDS             complement(7241..8845)
                     /locus_tag="PKK20_05845"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNU99440.1"
                     /translation="MKYIFVTGGVISSLGKGLTAASLGTLLENRGLKVTLQKFDPYLN
                     VDPGTMSPFQHGEVYVLDDGAETDLDLGHYERFTSTKLTRHNSLTSGQVYARVLERER
                     RGDYLGKTVQVIPHVTDEIKTRIRELAESTKADVVITEIGGTTGDIEGLPFLEAIREF
                     ALDVGYSNALFIHVTYVPFIKAAGELKTKPTQQSVAKLREIGIAPHLVVCRCDRPLNK
                     EIRDKISLFCNVPYEGVIEEKDVDHTIYEVPLMLQRERMDELVCQLLRLDTPPANMSH
                     WQDLLRKLIAPQKRIRIGVVGKYIGLQDAYKSVYEALSHGGIAHDCGIELERIEAEQI
                     EKEGAEKLLKGLGGVLVPGGFGERGVQGKILAAQYARESKLPFLGLCLGMQMATVEFA
                     RSVLELEGAHSTEFDKASPHPVIAMIEEQKGLMNVGGTMRLGAQPVQLQVGTKAARLY
                     GAFVVNERHRHRYEFNNSYRAQFEQRGCIFSGTSPDGELVELVELRDHPFFLGCQFHP
                     EFQSKPNAPHPLFRGFIAAALQRSSL"
     gene            complement(8870..>9236)
                     /locus_tag="PKK20_05850"
     CDS             complement(8870..>9236)
                     /locus_tag="PKK20_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNU99441.1"
                     /translation="RDPADYDNPNVVKVVVSSSGRALYFSRRTIPYLREAASRSIPDQ
                     LAAFAFLKHIGIYAYQRETLLRLVRWPVSALERAERLEQLRALENDVAIAVLRVDYES
                     VGVDTPEDAVRVERMLGSG"
BASE COUNT         1592 a         3077 c         2772 g         1795 t
ORIGIN      
        1 tgtggctgcc cgagccgttg cttgatctgc tgcgccaggc cgccgccatc atcggaggtc
       61 aaccatgaac ctgtccggcg tctccaccga actccgcccg gtggtcgagg acctcaaccg
      121 ccgattcgaa ggccggttca acgccctcgt caccccgcga ctcaacgagg tctacttcca
      181 cgtcacgatg gacctggtgc cgggcttctg cgcccacctc tgccgcaagt ggaaggcgcg
      241 cctggtgacc gtatttgccg acgacgcccg aattgggcat ggcgtgttcc atctttatta
      301 cgcgctggcc ctcgacgacg cgcacggact cctcgtcctc cgcgtccccg tttcgcccga
      361 aactccccag ttcgtcgccc tgaccaacgc catcccggcc gtcaattggc aggaacgcga
      421 gatacaggat tggttcggat tgaggctcct cggccatccc aatccacggc gctgcgccct
      481 ccacgacgac tggccggcgg tccatccgtt acgcaaggat ttcgacctca acaccgtgct
      541 gccccccttc cagggcgaac gccacaagtt ccgcccggtc gagggcgagg gcgtgtttca
      601 ggtgccggtc gggccggtcc atgcaggcat catcgagccg ggccatttcc tgttcagcgt
      661 ggcgggggaa ccggtgctct acttgcagtt gcgtctgttc tacgtccaca aaggcactga
      721 gaagcttttc gagcacctgc ccattcctca tggcgtgcgc ctggccgaga gcatctccgg
      781 cgattcgagc ttcgcgcatg cgacagcctt ctgccacgcc atcgagcgca ctgccggcgc
      841 tcaggcgcct ccgcgagcgc gtgtgctgcg gtcggtctgc ctcgaactgg aacgtttgta
      901 caaccacatc gccgacatcg gggcgatctg caccgatgtc gcgttcgtca cggccaacat
      961 gcacgccatg cggctcaagg agcaaatcct ccgcgtcaac gaacacctca ccgggaaccg
     1021 gctcctgcgc ggcatggcct gtctgggcgg ggtccggttc gattgggacg ccgaccagat
     1081 caggactctc aatggactgg taagcgatct gcggcccgag ttcgattcac tcgtccaact
     1141 cgtggaggac tcgtcgtcca cgctcgacag gctcgatacc acgggcatcc tcaaaccgga
     1201 ggtggcccgc gatctgggcg tggtcggcat cgcgggccgg gcgtccggat tcgcctacga
     1261 tctgcgccgc gacttcccgc acgccgccta tgatcaggtg caaatccggg tccccgtcta
     1321 ccgggtggga gacgtccgac accggctccg ggtccgcgtg gacgaagtgc acgagtccct
     1381 gaggcttatc caaacgttcg tcacaaagct gcctggcggt cctctgcgca tcgatatcgg
     1441 cgaagtgccg cccgaccggg tcgccttcgg ctacgtcgaa gggtggcgcg gcgagatctt
     1501 ccactgggtt cgaactgcgc ccaacaaccg actcgcacga tgccgcgtca aggatccgtc
     1561 tctccagaac tggccggccc tgagcgaggc aatcctggac aacattgtcc ccgacttccc
     1621 ggtcatcaac aaaagcttca acctgtcgta ttcgggcacc gaccgctgac ctatgccggg
     1681 cattctatcc aaatcgttgt cgacgggcat cgtcaccaca cgctatccgg acgcccccgc
     1741 ccacgcatcc ccccacgccc gcggtcggct cgagatcgac tggtccaagt ggaatgacgc
     1801 ccgctccgcc gccgccgtct gcccgactgg cgccattgcg tgccacgacg ccgcacaggc
     1861 acgcacggtc acattcgacc tcgccaagtg cgtcttctgc ggcctctgcg ccgacacgga
     1921 caaggcgatc cgcatgagca ccgtctgcga gtgcgccgcg cgtcaccgga accgcctcgt
     1981 ctcgaccgcg cagtacagcc tcaacgagga cggcacgcac cggcggttga tcgccggacc
     2041 cgacggggcc acagcgatgc gtggtgcagt ctcctccctt ccccgaaacg actcgatcga
     2101 gtcgctgggc cgccagattc agtcccgggc cgctcgcctc ttcggccgct cgctccacat
     2161 ccgcgaggtc gacgccggct cgtgcaacgg atgcgagatc gagatcgtcg gcctcaacag
     2221 cccggtctac gacatcgaac gtttcggcat tcacttcgtc gcgtcgccgc gccatgccga
     2281 cctgctgctg gtcaccggcc cggtgacacg gaacatggaa ctggcgctga agaagaccta
     2341 cgacgcgact cccgaacccc gcctggtggt cgccgtgggc gcctgcggct gcagcggcgg
     2401 catcttcggc gtcaactacg cgacgcgggg tcctgtcgac agcgtgattc ccgtcgatgt
     2461 ctacattccc ggctgcccgc ccaatcccca agccctgttg cacggaatcc tcctggccat
     2521 cgaccgcggc gtttagggtc tgagcataaa ttacttccga ttttgctgga ggggctttgg
     2581 ctcgctgacg cggcgcgact agggagcata cccgcagcgg gctgtgaccg atgagcaatc
     2641 ccgtgtccgc gggaccatcg agccaaagcc cccgcagccc ttcggggcga ggcggcaccg
     2701 ggccatctgc gtcgttcctc ggtcggtcga gtatctcagg ggatacactc cctccctcgg
     2761 gccttgcatc tggcccggtg gcgctcccgc caaaatcgga agtgattttt gcacagaccc
     2821 ttacctggcc ctccacgtgt ggccctacgc cgaaccatcg cagttcctac ccgcgacaat
     2881 tagcgtatct tccgacgcgt ccaagcccgc acgccctggg cgatctccgg gatgctctgc
     2941 atcgtcgccc tcacccgtcc cgcatggcgg cacaccctcg accatcggac ctgcctgccg
     3001 gagcctcggg gcaggcaggt gcgagagggt cgggctgacc gtgccgggga cgcggctcga
     3061 agaatctgtc tgggcaacgg tctcgagttc gagccaggct ggtctgcaat cgtttctgct
     3121 ggatttcggc gccgcagcca caccgactgc aatgggctcc gaaccgaacg atccggcgtg
     3181 gcattggaac aacattaccg aggcgattgg catgtcgcca gacggacgcc tgcccaagct
     3241 cgcaaccacg gcgaatctcc cgaccgacat tggcttcgag atgttgagtc gcttcaatgg
     3301 ggcagaccat gagggcagcg ccacatcgag cgcatttccc gccaacgcga cgcgcgactc
     3361 ggtctttggg aataccgaac ttttcaccgg ccttgccgac gtcttcccga gcttcaggtt
     3421 cgtccacctc acagcgcctc ggtcgcgccg ttgaggtcga cggcttcctc gatcagagcc
     3481 ccaacccgcg ccgtctcgct ctcgattcgg gtcaggggat ccctgaggtc gtgttcgatt
     3541 tcgagaaaca tccgatcgaa cacgagcgtg atctcgcgat ccaggcggtc gcggaaatca
     3601 tcccgggaat gtgccagcct ctgtgaaaac tcgcggagaa tcctcgaacg cttccagagc
     3661 agcgtcacgg cgaccaggcc ggcccccacg gcggcaagga tgccgcccgt aatgtcgaag
     3721 gcaatgaggt gagtcgccag ggcaatcacc gtccccagag cggccaggcc gccgccagcc
     3781 agggtgagcc ggcctaaatc ggagccctgg atttctttgt ccgacacgac atccgccacg
     3841 cgcagatcgc gcagttggca tcccagcgtc tcgagaatat cgccccgggc gcttgcgatc
     3901 ggcctgctct gcaggcgctg gccgttctga cgccgggcga ccgcctctct cagttcgagg
     3961 agcagcgcct gcatctcgtc cgacagatcc ttcgagaccc gcgaggactc cgcctcgatt
     4021 tcctccccgg ccgcacgctc gaattggaca tggagatcct tgagccaggt cttgacgtcc
     4081 ttgtcgcgga tcaggggcaa cgcgcgtcgc agaatggtcc ccacccccag gccctcgctg
     4141 aagtccgctt ccaggcggga cgacaggcgg tcgtagaccg cgcagagact gttgaccacc
     4201 aggcgggtga gggaattggc tttccgtcgg cgggcttcga gccggcccag gaggtcctga
     4261 tagaatgcgt tgtcctcggc gagcaaggac tgctctttgc gaagcctggc gagaaggtcc
     4321 gcaacagtct tgcgcagaat attcctcccc ccttcaagct tcattctcca gacatcgccg
     4381 ctttcgaccg cctggcgcag gtagttgcga aacgcggcaa acccggcgtc cgtgccctcc
     4441 tcggactcgc gtttcgcgga cagggggaac accaggggcg cctggacgcc ccgttcgcgc
     4501 gcgtactgct tgaccttctc gacattgatg tccagttcgt gctggctggc gcggtcggcc
     4561 tgctgcaaga caaacaccgt ctttcgatgc cattccttgc tgatgcggct gagcaggtcc
     4621 cactcgctgc cgcggtgggg attgctggcg agcaacacgt agatgaccag gtcgctgcgc
     4681 gggatgtact gttcggtgat tgtctggtga tgcgggatga cggagttgat gccgggcgta
     4741 tcgacgattg tgatctcacg caggctgggc ttcgggaggc ggattctctc ccactgctcg
     4801 cccaggctcg tcctcgagcg ctgttcgccg taggaaatct cctggatgac catggtgcag
     4861 ggatcgggtg ccacctcgca cacctcctcc cccaaaaggg cgttgacgaa gctgctcttc
     4921 cccgatttca cctcgcccac gatgacaaaa agggccgcgg actcgacatg tgccaaccga
     4981 tcccgcagca gggccgtcgc ccccgcctcc ccgcactgct cggtcagggc caggcagcgc
     5041 tggagcagga ttctgagcga ttcacactgt cgcttgaatg tcgggctgag gacgggctgg
     5101 ggaggcatgg cgatgtccct cggaatgcgg ccgcggcgga ggggtggacc tccgccgcgt
     5161 gccggactac ggggtggtcg gctggagcgg attggccctc ttgaggaatt tgaagaaatc
     5221 gctgtcggtg gtgaggatca gggatgtgtc gttggtgagc acggccttgt aggtgtccat
     5281 ggtcttgagg aactcgtaca aagccgccgc ctccgggctc tggttgtacg ctctggcgta
     5341 gatttccgac gcctccgcat cggcgtcccc ttggatttgt tgcgtctttc gatacgcctc
     5401 ggactcgatc gtctgcaggt ccttctcctt ttgacccaga atcttggcgg cttcgccggc
     5461 cccttccgaa cggaaccgct cggcgatctg ctgccgctcg ctgaccatgc gctcgtaaat
     5521 gcgggtccga acgctggtgt tgtagttgat ccgcttgaac tgcacgtcca tgagttcgat
     5581 gccgaacacc tcgatcttcg gagcggcatt ggcgagaatc tcctgctgaa ccttggcccg
     5641 gcccttgtgg atgggataga ggttcccgac gttgccgcgg gcgtcctcga tggccgcgtc
     5701 gcgctcgggc tcgcggtcct tggttgtgcg aatgacctcg atgagttcat gcctggcgat
     5761 tgcgttgcga atctcgctgc cgagaatgtc gtccaggcgc gacaacgcgc tccgttcatc
     5821 cttgaggcgc aggtaatact gtttggcatc gccgatccgc caccggccga aggcatcgac
     5881 aataatgtac gtcttgtcct tggtgggcat ctcgctcgga cgcccgtccc accgaaggac
     5941 gcgtttctcg atccggttca cctcctggat gaaaggcatt cggaggtgaa gtccggcatt
     6001 ggtgacggcc tggcccacgg gcctgccgaa ctgggtaatt atgacctgct cggtttcgct
     6061 caccgtatag gccgcggtca gcagcagaat gaacgccagg acgacgacga cgacgatcgg
     6121 gaggaggggg acggttggtt tcatggacgc ttgggctccg cgccgagttg cagcaggggc
     6181 agcggctgcg acgccttgtc atccaagatg atcttggacc gcacctgggg cagcacttcc
     6241 tccatcgttt caagataaat cctccggcgc gtcacctcgg gagccttgag gtactcggca
     6301 agcagcgcgt tgaaccgcgt ggcatcgccc tgtgcctcgt tgatgcgttt gaccgcgtaa
     6361 ccctccgccg cgctgatccg ctgttcggct tcgccgcggg cgcgcgggac ggccctgttg
     6421 tactcgccat tggccacatt gatggatttc tcccgctcct gctgcgcctg gttcacttcg
     6481 ttgaaggagg gctgcaccgg gatgggcggg ttgacgttct tgagttggac ctggtcgatg
     6541 cccacaccga tgccatactt tttcacaacc gcctgcaatc gcaccagcgc ctcgctctcg
     6601 atctcctgtc gtccgatggt gatcacctcg tccactgtcc ggtcgcccac cacttcgcgc
     6661 atgaccgatt cggacacgtc gcgcagggtc tcgcccggct ccctgaagtt gaacaggaag
     6721 gcccgcggat tattgattcg atactgaatg atccactcga cggtcgcggc gttgagatcg
     6781 cccgtcacca tgctccgttc cgcgtcctgc tcgtcggcag aaacgacctg ggtcggattg
     6841 ctgctcccaa acgctccgaa cccgaattcc tgcttgagct ggcgtcggac cggaacctca
     6901 aggacctggt ccagtccgta aggaagcttg aaatgcagcc cgggctcgac ggttttgaca
     6961 taccgtccga aacgcaggac gattccctgg gactccgcct gaacggtgta gatgaccgtg
     7021 ctcaggaggc tgatcgccag cgccgcaccc gccacaatca agagcaggcg cggacggatg
     7081 ttgtaatagg tattgcctac gcgtatttcc atcaccggat gcaccctcgc aaaacctcgt
     7141 ccgaggtcaa tcttcaatct tcacgccgac tgcccccgac gccccagggc tgcccgcgga
     7201 catccgatgg cgttcaccca atcactggcg ctcggaagga tcaaagactc gagcgctgca
     7261 gggcggcagc gatgaagccg cggaacaagg ggtgaggcgc gttgggtttg ctctgaaact
     7321 cggggtggaa ctggcaaccc aggaagaacg ggtgatcccg cagttcgacc agctcgacca
     7381 actcgccgtc gggcgatgtc ccgctgaaga tgcacccgcg ctgctcgaac tgcgcgcggt
     7441 agctgttgtt gaactcgtag cggtgccggt gccgttcgtt cacgacaaag gccccgtaca
     7501 ggcgggcagc cttcgtgcca acctgaagct ggaccggctg ggcccccaac ctcatggtgc
     7561 ccccgacatt catcagaccc ttctgctcct cgatcatggc gatgaccgga tgcggcgacg
     7621 ccttgtcaaa ctcggtcgag tgcgctccct caagctcgag cacactgcgc gcgaactcga
     7681 cagtggccat ctgcattcca agacagagcc cgaggaaggg cagtttcgac tcgcgggcgt
     7741 attgcgccgc caggattttg ccctgcacac cccgctcccc aaacccgcca ggaaccagga
     7801 cgccacccag tcctttgagc agcttctcgg cgccctcctt ctcgatctgt tcggcctcga
     7861 tccgttcgag ttcgatcccg caatcgtggg cgatgccgcc gtggctcaga gcttcgtaga
     7921 ccgatttgta ggcgtcctgc agtccgatat acttgcccac caccccgatg cgaatccgtt
     7981 tttgcggggc gatgagtttg cggagcaggt cctgccaatg cgacatgttg gcgggcgggg
     8041 tatcgaggcg caggagttgg cacaccaact cgtccatccg ctcgcgctgc agcatgagcg
     8101 gcacctcata gatggtgtga tccacgtcct tttcttcgat caccccttcg tacggcacgt
     8161 tgcagaaaag cgagatcttg tcccggatct ccttgttgag cgggcggtcg caccgacaca
     8221 ccaccaggtg cggcgcaatc ccgatttcgc gcagtttggc gaccgattgc tgggtcggct
     8281 tggttttgag ttcgcccgcc gccttgatga acggaacata ggtgacgtga atgaacaggg
     8341 cattgctgta tccgacgtcg agggcgaact cgcggatcgc ctcgagaaac ggcaaacctt
     8401 cgatgtctcc cgtggtgccg ccaatctcgg tgatgaccac gtcggccttg gtggactcgg
     8461 ccaattcgcg gattcgcgtc ttgatctcgt cggtcacgtg gggaatcacc tgcacggtct
     8521 tgcccaggta atccccgcgc cgctcccgtt cgagcacccg ggcatagacc tggccgctgg
     8581 tgagactgtt atggcgcgtc agcttggtac tggtgaagcg ctcgtaatgg ccgaggtcca
     8641 ggtcggtctc ggccccgtca tcgagcacgt aaacctcccc atgttggaac gggctcatgg
     8701 tgcccggatc cacattcagg tacgggtcga atttttggag cgtgaccttc agcccacggt
     8761 tctcgagcag cgtgcccaac gatgccgcgg tgagtccctt gcccaacgaa ctgatcaccc
     8821 cgcctgtgac aaagatgtat ttcatgcctg agaaatcggt cctggtcccc taccccgacc
     8881 cgagcatccg ctccacccgc acggcgtcct ccggcgtatc cacgcccacg ctttcatagt
     8941 ccacacgcag cacggcgatt gcgacatcgt tctccagcgc ccgcaactgc tcgagccgct
     9001 ccgcccgctc gagggcggac accggccatc ggacaagtcg caacagcgtt tcacgttgat
     9061 aagcgtagat gccgatgtgc ttcagaaaag caaacgccgc caactgatcg ggaatcgaac
     9121 gactggcggc ctctcgcaga tacggaatcg tgcgccggga gaaatatagg gctcgcccgc
     9181 tcgagctgac aacgaccttt acgacgttcg ggttgtcgta atcggcggga tcgcgg
//
LOCUS       DAOQRO010000112         9186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_6967_length_9186_cov_4.293396, whole genome shotgun sequence.
ACCESSION   DAOQRO010000112 DAOQRO010000000
VERSION     DAOQRO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6967_length_9186_cov_4.293396"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..963)
                     /locus_tag="PKK20_05855"
     CDS             complement(247..963)
                     /locus_tag="PKK20_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNU99442.1"
                     /translation="MRTLPLKPPTDEVDWPMILAVLGLMTIGTLFIASTTLAREGAET
                     LPWHRQEHYKQLIWYALGAGLGVAVCCLGHSPIARWSMVCYWVSILLLVTVLIPGIGT
                     QHGWEARRWIDLGPFQFQPSEVAKLTFLLMMAHYLSRPAAELRQPMTFVKALGMSLLP
                     CALILKEPDLGSALVFVPMGLAMMFVAGVPGRFLWRLIGGAGLIIVLLLADILFAPSE
                     WQLKLEPYQRRRQKRNPCSA"
     gene            complement(965..2938)
                     /locus_tag="PKK20_05860"
     CDS             complement(965..2938)
                     /locus_tag="PKK20_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNU99443.1"
                     /translation="MFFDQFRKGGERLQTLAIGITLGLLLLLAGLWHIQIVSAKRYRA
                     SLETQTYRTVRIPAIRGKLLDRDGRVLAENRPSYNLNLYLEELRPQFQEAFTKARAGR
                     RLTRAERAQLAVEVRWLVASNTIDRISRFLGEASTITDRDFRLHHHQWPYRPLAVREN
                     LSPAQIARFMENAPNLPGLDIEVQPLRHYPCGSMAGHLLGYLTRDDMARDDDEGGFSY
                     SLPSYEGAVGLEYLFDDELRGKPGTKSVVVNSLCYREGEMIWLTASPGHNAVLALDLP
                     MQQAAFEALRSLGPGTRGALVVMDTTNGDVLAMVSSPAYDPNEFVSPLSHERWAVLND
                     PRLRPMFNRTTQGAYNPGSIFKIITALAALEHGLDPHAQIQVQPDPRRPGRGAIFLGR
                     RKIDDTVPPGDYDFKRAFMHSSNSYFVQVGLQTGRDRLLAMGQQFLLGRHFGLGTRQE
                     VSGYFPSPQDVLGIWTEGNLANACIGQEITVTPLQMAVMTAAVANGGKLLRPRLVHRL
                     ERAEPGPEGAVAKAFPPRLLGELKVHARSLATVREAMLADTEEPGGTGFAAFQQKNPL
                     TGQITPRIPGFRVAGKTGTAQVKKGATVIDHITWFVAFGPYDNARYAVVVMVESGDSG
                     GRTCAPVACRVFQALKQRFEPAPATAPRLVSRK"
     gene            complement(2944..3495)
                     /locus_tag="PKK20_05865"
     CDS             complement(2944..3495)
                     /locus_tag="PKK20_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99444.1"
                     /translation="MNGPQTLILLVLALLGVFAEAVVDFPRVWLRTQFDVLPPLMVYA
                     SLTGGVGTLVLLATVGGICADALSGNPMGVSVLPLFWVGLALHWRRELILRDLPYAQF
                     VLGGMAAAAVPVLTLVVLLTLGENSLLGWGTLWQLLVVTIGGAALTPLCFSVMETLKR
                     AFAYQPSTTTPFPANREIKRGRY"
     gene            complement(3492..4298)
                     /gene="mreC"
                     /locus_tag="PKK20_05870"
     CDS             complement(3492..4298)
                     /gene="mreC"
                     /locus_tag="PKK20_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNU99445.1"
                     /translation="MFRRPYPLAFVVVLALVLVILNLPQAVATKAKLAVGSLFLPLFG
                     LATLTRDLGQQAGKHLPPPSALIAELDRTRRENRELRVLVAQGDEALRENDRLRQLLG
                     WKPQLPWATKPARIIARDTANWWRTARIDRGTRDDLRPNLPVVSADGLVGKIGEVGLT
                     TAEVVFVGDPKCRVAVVVRETSEHGVLSSSSVGVLDHRLMDLTHLPRHTALKPGQRVF
                     TSGLGGVFPAGLPVGTIVDYRSVGYGLYTEARVKLSADTSRLNEVLVLFP"
     gene            complement(4352..5377)
                     /locus_tag="PKK20_05875"
     CDS             complement(4352..5377)
                     /locus_tag="PKK20_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNU99446.1"
                     /translation="MFAQLKGLFSNDIGIDLGTANSLVYVRDRGIVLREPSVVAIQAG
                     TNNVLAVGEEAKRMLGRTPGNIIAIRPMKDGVIADFDVTGAMLRHFIQRVHHRKLIAP
                     RVVVAVPSGITEVEKRAVKDSVTHAGAREVYLIEQPMASAIGVGLPVHEPAGNMIVDI
                     GGGTCEMAIISLAGIVFSRSLRVGGDEFDETIVAHMKRAHNLMIGERTAEEIKLRIGS
                     AYPLEQELTMDVKGRDLSTGLPKTITVRSEEIREALKEPLSSILEAIRITLERCPPEL
                     AADLVDRGVVMAGGGALLRGVDRLVAEETYLPVHIADDPLTAVAEGTGRVLQELQFLK
                     RVASSST"
     gene            complement(5518..6729)
                     /locus_tag="PKK20_05880"
     CDS             complement(5518..6729)
                     /locus_tag="PKK20_05880"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNU99447.1"
                     /translation="MKAKGMVYLVGAGPGDPQLLTLRGAALLRRADTVVYDGLVSPDL
                     LKLAPPAAERIERTEERFPDQAALGALLAARARAGQCVVRLQGGDPRVSGQGSSEAQG
                     LAAAEVPFQVVPGVASIVSALDRSSTSTSEEPSPKTSKTWEHMPLLGQRVVVTRARKQ
                     AFEFVQLLQERGAHVLEVPCIRIVPPTERKPLIEAIAGLGCYDWLIFTSANGVAAFFE
                     HFFVAFDDLRDLGAVRLAAVGPATAARLKELHLKIDLMPKEYIASKIARALCKEQSLE
                     NIRVLLLRAEAANADLPRLLEDMGAIVDDVACYGTAWETEDAAGHGALLAESGADWIT
                     FTSGSTVESFNARFDLRALMRRFPKLRLATIGPETSKALEALSLHPSLEAKTHTIAGL
                     TSALERARRMV"
     gene            6902..7654
                     /gene="rph"
                     /locus_tag="PKK20_05885"
     CDS             6902..7654
                     /gene="rph"
                     /locus_tag="PKK20_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNU99448.1"
                     /translation="MESQLLRSDGRRDDELRPISFQNGIAPYATGSTLVSWGNTRVIC
                     GVTIDEAIPRWMKEQGVPGGWITAEYSMLPYSTLDRKPRDISKGRLDGRSQEIQRLIG
                     RAMRAAVELDRLGPRTVWIDCDVLQADGGTRTAAITGSYVALHLAIQKLLADGKLTSN
                     PLSDSVAAVSLGVVDQRVLLDLNYEEDSKAEADMNLVMTGRGEFIEVQVTGEQNTFSQ
                     LQLESLLALGRAGVNRIIAAQNAALGTRPTSL"
     gene            7666..8580
                     /locus_tag="PKK20_05890"
     CDS             7666..8580
                     /locus_tag="PKK20_05890"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNU99449.1"
                     /translation="MAADSIEVSVVTSIDAEEAVAALLERVSGLAPVLYTARERPTTK
                     VTVYLDRRHRWTDARKKAVLRGLTLLRAEGLDVGPGRLRLRRLRAESWVNAWKRHFRP
                     IEIGDALLLKPSWSRRSPKAAQAVVTLDPGLSFGTGQHPTTRFCLEQIVACRNQAPPP
                     SLLDAGTGSGILAIAAAKLGYRPILAFDCDPASIKAARANARANNVSARARFLRGDLR
                     RPPIEPGARFDVVCANLTTDILMVEAKRIVQWIRLGGRLVLAGILAVEFHDVFKAYER
                     LGLKLVTTRVEREWQCGAFARTRQRKPL"
     gene            8735..8896
                     /locus_tag="PKK20_05895"
     CDS             8735..8896
                     /locus_tag="PKK20_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99450.1"
                     /translation="MPKSNDIIANAVSNPKHPFPHVDDRPGKQKKHRYERRKVKEYIK
                     LGDWSEQTA"
BASE COUNT         1768 a         2923 c         2889 g         1606 t
ORIGIN      
        1 taccgcttac ggcatacact tgagtctaga agacattgcc gtgtgatacg ctttgggtga
       61 tctggatttt cgcgtcccgg aaatcaactg gcgtacaccg catctcagcc tggccagccc
      121 gcgcgagaat ctgaactacc gcccgagctt aggggactca gtacccgcgg gttcaacaca
      181 cgttgtgact atcgatgagc gtactctacc gggatcattt cgagttcacg accgacccct
      241 tcggaattac gccgaacacg gatttctttt ttggcggcgg cgctggtaag gctcgagctt
      301 gagctgccac tcggatggcg cgaagaggat gtcggcgagc aggagaacaa tgatcaggcc
      361 tgcgcctccg atgaggcgcc ataggaagcg gcccggcact cctgcgacga acatcatcgc
      421 caatcccatc gggacaaaca ccagcgcgga gccgagatca ggctccttga ggatcagcgc
      481 gcagggcagc aggctcattc ccagggcctt cacgaaggtc atgggctgcc gcagttcggc
      541 ggcgggtcgg ctcaggtaat gcgccatcat cagaagaaaa gtcaacttgg cgacctcgga
      601 cggctggaac tggaaaggcc ccaggtcgat ccaccgtcgc gcctcccatc cgtgttgggt
      661 tccgatcccg gggatgagga ccgtgaccag gaggaggatg gagacccagt aacagaccat
      721 cgaccatcgc gcgatcgggc tgtgacccag acagcacacg gcgacgccca agcccgctcc
      781 cagggcatac cagatcaact gtttgtaatg ctcctgtcta tgccagggca gagtctcggc
      841 gccttcccgc gccagggtgg tgctcgcgat gaacaacgtc ccgatcgtca tcagtcccag
      901 aacggccaga atcatcggcc aatcgacctc gtctgtcggc ggcttgaggg gcagcgtgcg
      961 catcttactt gcgggaaacc aaccgcggcg cggtggccgg agccggttcg aaccgctgct
     1021 tgagggcctg aaatacccga caggcaaccg gcgcgcacgt gcggccgccc gaatccccgc
     1081 tttcgaccat gacgaccacg gcgtagcggg cgttgtcata cgggccgaac gcgacgaacc
     1141 atgtgatgtg gtcaatcacg gtcgctccct tcttaacctg tgccgtgcct gtcttaccgg
     1201 cgacgcgaaa gccgggaatc cttggcgtga tttggcctgt cagcgggttc ttctgctgga
     1261 aagcggcgaa gcccgtgccg cccggctcct cggtatcggc cagcatcgcc tcgcgcacgg
     1321 tggccagtga gcgcgcgtgg accttgagct cgccaagcag gcggggaggg aaagccttgg
     1381 cgacggcgcc ttccggtccc ggctcagccc gctcgaggcg gtgaaccagt cgggggcgga
     1441 gcagtttgcc gccgttggcg acggccgcgg tcatgaccgc catctggagc ggggtgacgg
     1501 tgatctcctg gccgatacac gcattggcca agttgccttc agtccaaatc cctaggacat
     1561 cctgcggaga ggggaagtag ccgctgactt cctggcgcgt gcccagaccg aagtgcctgc
     1621 cgagcaggaa ttgctggccc atggccagca gccggtcgcg tccggtctgc aaaccgacct
     1681 ggacgaagta ggaattgctc gagtgcatga atgcccgctt gaagtcgtag tctcccggtg
     1741 ggaccgtgtc gtcgatcttg cggcgtccaa ggaagatcgc tccgcgtccg gggcgtcggg
     1801 gatcgggttg gacctggatt tgggcatggg gatcgagacc gtgttcgagt gcggcgagtg
     1861 cggtgatgat cttgaagatc gagccggggt tgtaagcgcc ctgtgtggtc cggttgaaca
     1921 tggggcgcag acgcggatcg ttgaggacgg cccagcgttc gtgcgagagg gggctgacga
     1981 actcgttggg atcgtaggcc gggctcgaga ccattgccaa cacgtcgccg ttcgtcgtat
     2041 ccatgacaac aagagcgccg cgggtgccgg ggccgaggga gcggagcgcc tcgaaagcgg
     2101 cctgctgcat cgggagatcg agcgcgagca ccgcattgtg ccccggcgag gccgtgagcc
     2161 agatcatctc gccctcgcgg taacagagac tgttgactac gaccgatttg gtgccgggct
     2221 ttccccgcag ctcgtcgtcg aagaggtatt ccaggcccac ggcaccctcg tagctgggaa
     2281 ggctgtaact gaatccgccc tcgtcatcgt cccgcgccat gtcgtcccgg gtaaggtatc
     2341 cgaggaggtg ccccgccatc gaaccgcaag gatagtggcg cagcggctgc acctcgatat
     2401 cgagccccgg gaggttcggc gcgttctcca tgaaccgcgc aatctgggcg ggactgaggt
     2461 tttcgcggac ggccaagggg cgatagggcc actggtggtg atggaggcga aaatcgcggt
     2521 cggtaatcgt gctcgcttcg ccgaggaagc gcgagatgcg atcgatggta ttgctggcga
     2581 cgagccagcg aacctccacg gcgagctgcg cgcgctcggc tcgcgtcagt ctgcgtccgg
     2641 cgcgggcttt ggtgaacgcc tcctggaact gagggcggag ttcttcaagg tagaggttga
     2701 gattgtagct cgggcggttc tcggcaagca cccgcccgtc ccggtccagc agcttgccgc
     2761 ggatggccgg gatgcggacg gttcggtagg tctgagtctc gagactggcg cgatagcgct
     2821 tggccgagac gatctggata tgccacaagc cggccaggag caggagcaaa ccgagcgtga
     2881 tgccgatcgc cagcgtctgg aggcgctcgc cgcccttgcg aaattggtcg aaaaacaaca
     2941 cggttagtat cgtccgcgtt tgatctcgcg gttggcggga aagggggttg tcgtcgacgg
     3001 ttgataggcg aacgcgcgct tcaacgtttc catgacgctg aagcagagcg gcgtcagggc
     3061 ggctcctcca atcgtgacga ccaggagctg ccagagcgtt ccccacccga gcagggagtt
     3121 ttctcccaag gtcagaagca ccaccagcgt gaggaccggg acggccgccg cagccatccc
     3181 gcccaggacg aattgcgcgt aaggcaggtc ccgcagaatc agctcgcggc gccaatggag
     3241 cgccaggccg acccagaaca gaggcaggac gctcacgccc atcggattgc ctgagagcgc
     3301 gtcggcgcag atgccgccca cggtggcgag cagaacaagg gtgccaacgc cgccggtgag
     3361 ggatgcgtaa accatcagcg gaggcaaaac atcaaattgg gtgcgcagcc agacccgtgg
     3421 aaaatcaacc acggcctcgg caaacacgcc gagcagggcg agcaccagaa ggatcagtgt
     3481 ctgggggccg ttcatgggaa caggaccaag acctcgttga gccggctcgt atcggccgac
     3541 agtttgaccc gcgcttcggt gtagagcccg tatccgacgc tgcggtaatc gacgattgtt
     3601 cccacaggca aaccggccgg aaagacgccg ccaagcccgc tggtgaagac gcgctggcca
     3661 ggcttgagcg cggtgtgacg cggcaaatgg gtgagatcca tcaggcgatg gtcgagaaca
     3721 cccaccgagg aactcgaaag cacgccgtgc tcggacgtct cgcgcacaac aaccgccacc
     3781 cggcatttcg gatcaccgac aaacacgacc tcggcggtgg tgagtccaac ctcgccgatc
     3841 ttgccgacca ggccgtcggc gctcactacg gggagattcg gcctgaggtc gtcccgtgtg
     3901 ccacggtcga tccgtgcggt gcgccaccag tttgcggtgt cccgcgcgat gatccgggcg
     3961 ggcttggttg cccagggaag ctgcggcttc cagccaagca gttgtcgcag ccggtcgttt
     4021 tcgcggaggg cctcgtctcc ctgtgccacc agcacacgca actcccgatt ctcgcgacgg
     4081 gtccgatcga gctcggcgat gagcgcgctg gggggtggaa ggtgcttgcc ggcctgctgc
     4141 ccgaggtcgc gcgtcaaggt ggcaagaccg aacagaggca gaaagaggct gcctactgct
     4201 agtttcgcct ttgtggcgac ggcctgtggc aggttcagga tcaccagcac cagcgccaag
     4261 accaccacaa aggccaaagg atacggcctt ctgaacattc gttaccccgt aactgctgtg
     4321 cggggcgaac gcgcggtctt cgtgcagacc cttaggtgga ggaagaagca acgcgcttca
     4381 ggaattggag ctcttgcagg acccgtccgg tcccctcggc tactgccgtg agcggatcgt
     4441 ccgcaatgtg gaccggaaga taggtctcct cggccaccaa acgatccaca cctcgcagga
     4501 gcgccccgcc acctgccatg acgacgcctc gatcgacgag gtccgccgcc aactcgggcg
     4561 ggcaccgttc caaggtgatg cgaatggctt ccaaaatgct ggaaagaggt tccttcaggg
     4621 cttcccgtat ttcttcggag cgcaccgtga tcgtcttggg cagacctgtg ctcaggtcgc
     4681 gtcccttcac gtccatggtc aattcctgct ccagaggata agccgatccg attcgaagct
     4741 tgatttcctc cgccgtacgt tcgccaatca ttaagttgtg ggcacgcttc atgtgcgcga
     4801 cgatcgtttc gtcgaactcg tcgcctccca cgcgcagact gcggctgaac acgatgccgg
     4861 ccagggagat aatggccatc tcgcatgtgc cgccgccgat gtcgacaatc atgttgccgg
     4921 cgggttcatg cacgggcagg ccgacgccga tcgccgaggc catcggctgc tcgatcaaat
     4981 acacttctcg cgcaccggcg tgggtcaccg agtccttgac cgcccgtttc tcgacctctg
     5041 tgatgcccga tgggacggcc accacaactc gcggcgcgat cagcttccga tggtgcaccc
     5101 gctgaatgaa gtgccgaagc atggccccgg tgacatcgaa atccgcaatc actccatcct
     5161 tcatcggccg gatggcgatg atgttgcccg gggtcctgcc gagcatgcgc ttggcctcct
     5221 cgcctacggc cagcacgttg ttggtgccgg cctggatcgc cacaaccgac ggctcgcgaa
     5281 gcacaatccc ccgatcccgg acgtacacaa gcgaattcgc cgtcccgaga tcgatgccaa
     5341 tatcgttgga aaacaggcct ttaagttgcg cgaacatgaa agctgcctaa cacgccccgg
     5401 acattagtgg ccacacgcct cgccggtcaa gattattaac gttcacccgt ccgtgcggag
     5461 ccgtgtccgg acagtctccc ggccgaccct catgcagcaa acctcgcgcg catggattca
     5521 caccattcgc ctggcccgct ccagggcgga tgtcagacct gcgatcgtat gcgtcttggc
     5581 ctcgaggctg ggatgcagcg acagcgcctc gagggctttg ctggtctcgg gaccgatcgt
     5641 cgccagtcgc agcttcggaa accggcgcat cagcgcccgc agatcgaaac gggcattgaa
     5701 gctctcgacg gtcgagccgc tggtgaacgt gatccaatcc gctccgctct cggccaggag
     5761 cgccccatgc cccgccgcat cctcggtctc ccacgccgtg ccgtagcagg ccacgtcatc
     5821 cacgatcgct cccatgtctt cgagcagcct gggcagatcg gcgttggcgg cctctgcgcg
     5881 caacaggagc acgcgtatat tctcgagact ctgctccttg cacagtgccc tggcgatctt
     5941 cgacgcgatg tactccttgg gcatcagatc gatcttgaga tgcagttcct tcagcctggc
     6001 agccgtggcg gggccgaccg ccgccaaacg caccgcgccg agatcgcgaa gatcgtcgaa
     6061 cgccacgaag aagtgttcga agaaagccgc gactccgttg gcgctggtga aaatcagcca
     6121 atcatagcag cccaacccag cgatcgcctc gatcaacggc ttccgttccg tcggcggcac
     6181 gatccgaatg cacggcactt cgagcacgtg cgccccccgt tcctgaagga gttggacgaa
     6241 ttcgaacgcc tgcttgcgcg cgcgcgtcac cactacacgc tggccgagaa gcggcatgtg
     6301 ctcccacgtc ttcgacgtct tcggcgacgg ctcctcagac gtcgaagtgc tcgatcggtc
     6361 gagcgccgat acgattgaag caaccccagg caccacctgg aatggaacct ccgcggccgc
     6421 cagcccctgt gcctccgagc tgccctggcc cgaaacacgc gggtcgcctc cctgaagccg
     6481 gaccacacac tggccggccc tagcccgcgc cgccagcagg gcgcccagcg cggcctggtc
     6541 cgggaatcgc tcctcggtgc gctcgatgcg ctcggcagcg ggcggcgcca gcttgagcaa
     6601 gtcggggctc accagcccgt catagaccac ggtatccgca cgacgcagca acgctgcgcc
     6661 tcgcagcgtc aacagttgcg gatcgccggg gcccgcgccc acaaggtaca ccatgccctt
     6721 tgccttcatc ggcggcgacc ataaaccccg tcgcccgcaa tatccagacc tcgccgcgag
     6781 cgattgcggt gcgtcaggtc gttgtcggcg gtttcctcat tgcctgcccg cccgagcctg
     6841 attagtgtcc gccgttgtgg aagtgcaacc aaagaccgac aatcccgcca cccgcggtcc
     6901 tgtggaatct caactgctgc ggagcgacgg acgccgcgac gacgaactgc gtccgatcag
     6961 tttccaaaac ggcatcgcgc cctatgccac aggatcgact ctggtttcct ggggcaacac
     7021 ccgtgttatt tgcggcgtca cgatcgatga ggcgatcccg cgttggatga aggagcaggg
     7081 cgtgcccggc ggctggatca ccgccgagta ctcgatgctc ccctactcga cgctcgaccg
     7141 caaaccgcgc gatatcagca agggccgact cgacggacgc tcgcaggaga ttcagagact
     7201 gatcgggcgc gccatgcggg cggcggtcga actggatcgg ctaggaccgc gaaccgtctg
     7261 gatcgactgt gacgttttgc aggccgacgg cggcacgcgc acggctgcca tcacgggcag
     7321 ttacgtggcc ctgcacctgg ctattcagaa acttctggcc gatggcaagc tgacctcgaa
     7381 cccactgtcc gattcggtcg ccgccgtcag tctgggcgtc gtcgaccaac gcgtcctgct
     7441 cgatctgaac tacgaggaag actcgaaggc ggaggcggac atgaacctgg tcatgaccgg
     7501 gcggggcgag ttcattgaag tccaggtgac cggcgagcaa aataccttca gccaactgca
     7561 gctcgagtcc ttgttggctc tcggacgagc gggagtcaac cggatcatcg ccgctcagaa
     7621 cgcggccctc gggactcgac ctacaagcct gtagagcgca tctcgatggc agccgattca
     7681 atcgaggttt ccgttgtcac ctccatcgat gccgaggaag ccgtggcggc gctcctcgag
     7741 cgggtgtccg gtctggcgcc cgtcctctac actgcgcggg agaggccgac gacaaaggtc
     7801 accgtatacc tcgatcggag acaccgatgg accgacgcca ggaaaaaagc cgtgctgcgc
     7861 ggcctgacgc tcctgcgcgc cgaaggcctc gacgtcggcc ccggtcggct tcggctgcgc
     7921 cggcttcgag ccgagagctg ggtgaacgcc tggaaacgcc attttcgtcc aatcgagatc
     7981 ggcgacgccc ttctgctcaa gccgtcctgg agccggcgaa gccccaaggc cgcccaggcc
     8041 gttgtcacgc tcgaccccgg actgagcttc ggaacaggcc agcaccccac aacccggttc
     8101 tgtctggaac agatagtggc ctgccgcaac caggcgcctc cgccatcact cctggacgcc
     8161 gggacggggt ccggcatcct ggccattgcc gccgccaaac tcggctacag accgatcctg
     8221 gccttcgact gtgatccggc ctcgatcaag gctgcccggg ccaatgcccg cgcgaacaac
     8281 gtgtccgcac gagcgcggtt tttacggggc gacctccggc gtcctccgat cgagccaggc
     8341 gcccggtttg atgtcgtttg cgcgaatctg acgaccgata tcttaatggt tgaggccaag
     8401 cggattgtgc agtggattcg tctcggaggg cggctggttt tggcgggcat tctggccgtc
     8461 gagtttcacg atgttttcaa ggcgtatgag cgcctggggc tgaagttggt gacgacacgg
     8521 gtcgagcgcg agtggcagtg cggtgcgttc gctcgaaccc ggcaaaggaa gcctctgtaa
     8581 agccgcttga acgaaatcgg cggacacgtg tcgataccac tgtgcacgtg gagcctgagt
     8641 ggccatgcag caggcaaaac aaggcgaaac cacaggatgg tgggaacgcc gggggtactt
     8701 ctcccctccc tcaggttcaa ttcagtccag cactatgcca aagtcaaacg acatcatcgc
     8761 caatgcggtc agcaacccca aacatccatt cccacacgtc gatgaccgcc cgggtaaaca
     8821 gaagaaacac cggtacgaac gacgtaaggt caaggaatac atcaaactcg gcgattggtc
     8881 cgaacaaaca gcctgactga cttcattcat ccaatagctg gttgcgattt gaaagggcca
     8941 cgccggcagg cgtggccttc tttttgcgcc tggtccttac tgaggaatca gcgagatgca
     9001 ctacaccgtt accctcaccc gtcctccgga caccctctcc cacctgcctg cgtccaggct
     9061 tcggcaggca ggtcggatgg gagaaggacg gggtgagggg tctgtgttca agcaaaaaat
     9121 cgcttcggac gacaaaaagc agggctgctc taaagtcgaa ccgggcgggt gagtaggcga
     9181 atgacc
//
LOCUS       DAOQRO010000113         9047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7119_length_9047_cov_4.037478, whole genome shotgun sequence.
ACCESSION   DAOQRO010000113 DAOQRO010000000
VERSION     DAOQRO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7119_length_9047_cov_4.037478"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>9047
                     /locus_tag="PKK20_05900"
     CDS             <1..>9047
                     /locus_tag="PKK20_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNU99451.1"
                     /translation="VRLVAVNPVATQPFNLGVDLIAQGDENSVGFSLQFDVARLEYMD
                     ATAGSGGFSLLVNKSRAAQGQLGFALGRSAGTVFPSGTIRMVNVEFRARETTGTTPLA
                     FADVPVVRETVGVQAQTLTTLYEGLSLEILPLVGPSITTQPEGATVHAGTNFTLRVVA
                     AGSEPLRYQWQLNGQPLASATNATLELSSIRESQAGDYRVGVSNPAGSVTSAVARLVV
                     LPPLTAPVIVRNPKPAFASAGERVTFTVEATGSEALSYQWQRQGIDLAGATEPVLVID
                     AVTTAHAGNYRVLVRNPVGSATSTAVALTVSATPRAIRIGRVDSPAGNLIELPIDLYA
                     LGDENAVGFSLTFDPAQLEYRGWRRGATAQGTALNVNTNGLAQGRLGLAISKPAGQRF
                     SSGTSPLVYLQFLVGRTPSQTDILAADAPVRQEIADVGGRSRPAEFVDGWVNILDTPP
                     SIVSAPADTVARLFSTVTLEVVANGSAPLYYEWRHNGQPVPGATSPTLTLASVMPDQA
                     GDYQVVVSNAVAIAGSDTARLEVRRVVRLAGTNIPTGVTVDMPVELLTAGTENAVGFS
                     LRYDPTLLTLQSAKLNPGFTNVTLNLRTNASFPGALGVALAQPPGGVFPTGTQAVMTL
                     TFQATQLPGNCPLTFTDMPILREMANADAQSVPTDFVDGEIITTRVAPQITRHPVPRE
                     VTQGESAVFAVVASGSLPMTYQWLHNGVDLPGATNTTFSIVAVQPVDAGNYAVRLSNV
                     ASTVISSNALLTVNIPDMTGPVLANPTFDSQPLAPDALLTRAGTLRIDATDPSGVSRV
                     EFYVDDLQVSADLDGADGFTAYLDFELFPDGPHTLSFRALDFRSNPAEFTLPVRFLLA
                     TLPPPVIGLPQSGTITSNQFVTIRGNAPAHSSVVLYRNDARAGNPVPVNHLGIFETTL
                     PLLEGLNRLEAASLNRAGESQRSPEVTVTLDTSLPASPSGLRATARSGGRVLLEWVPS
                     SESYRGYHVYRSHASFTDRSEAVRLTGSPLPGYTFEDTAPVDGQWFYRIAQVNLANVE
                     GLLSIEAAVEVDRTPPTAALEYQPPAAYDPATGRVGRGIVGVLVNLSEEVASAPFLSF
                     MPPQGQPIVIELRRVDPTQYRGNFVIDDKTPSGNAVAVFSARDKAGNRGTQITAGARL
                     TIDTAAPEIVGLTVQPAEPIRNPADETVLVTLDLTLSEVLRTAAAPEITYSLSESAPT
                     PQPVASLTPGADPVHWTVTFKLPAAAGANAETLTIQYQGEDDLGNRGSRIMPPHQFQV
                     YQGDLPALVAPVSLSAKTKSAGLIVLEWQRVTDASDYAIYRKTPSATEFEPLTYTGDT
                     NRWSDLPPEDGIYEYAVASVRRVNAQESVSELSDVAIAESDRRAPGAPSDLTLQLAKN
                     GVFLRWTSPVNLNEEITYSLYRTVQTPPADLTGLTPAISGIPIAQVVDPAPRPDQPFY
                     AVAAVDRAGNVSPPSNPGYLNIQLYPVQSLTVGQTNNDPPILTWTQVGSGVAGHDIFL
                     GDANSNLHLNRRGLITDNRFIDIGFTGEERLYTIFTVDNNGQRSPGRTILLPALSAAL
                     DPDAIVKRGLMNRLTYTVRNDSTDPLNEARVLVTLGGRVHSSETFWLAGQSVVEIPVI
                     VGGYDELPDGNAPLLTTIESRPEEGATVRWARSGSVAVGDGQLLIGVLASDLFRGAIT
                     KARFTLLNPSTEVLEVITATQQGKAPSSDIRMSLFNAESQLLSSTPFFAAMGTNLVLL
                     PNGNIVLRLEPGQEVVSQEIDLPVPSSVPNHVWIQLEIDRVFYHSDREDRVEMRGVAT
                     AAPFVVTSTSYTGTITSVSPEHSRGGEPITISGRAWFQASRQPAPRQPLLVKIANAGF
                     ERTEQVVTDDQGLFTTQFTPLAGESGGIYSVWAVHPDLTDRTVQKQFVVDRVLASPTE
                     FVVRAPHYYRQVVPLKVTTGAGTEVANLRLAFLPQDQPGRALPPGVAVDTGAPIASLG
                     PNQTASMSFLVTGTPEATRKVDLVLRLVSGETVDNPWATIRAACEFSEAYPSLRWSPS
                     LVDTGAPPGSNVVEEVTFENVGLVSATNVFFSLHKADGTAAPSWAALATPPNLAELAV
                     GAKAIVGLTFRPPSALPEDDYWFKLKTRSGNHPTVEIGVHVAVLAEGRGNLAFKVVDM
                     YTGTPGNDGVSGANIRLRNDAVGSFQTNLVTDINGEAVFEDLPSGLYDYHVTGENHNS
                     SNGRAWVRAGSTSSQHVALAYSLVSVEWEVVPITIEDRYEIVLTAVFETDVPAPVVTI
                     EPAAVNLPQLFAGDVFYGEYVIENHGLIRADNLQFQLPASDAYLKYEILAAVPDRLEA
                     KQKVRIPYRITCLTSFPGPRTPPPTANAALARTRTTAQRPSAVNLDGLPPAIRQIVLM
                     DKPSGICFTHTSGTSLMYEWICINGEWYRNAIGSQFWYNYSSGPCGTPGGGATWVGGG
                     GATFSGPSASIPGLPECCPPEECEEEDPCDPKCCEAEGATCDSSPPPPGTPGSPGSEG
                     SGGPDDSDQRVRLANAGGQAGARFKFINQLRVEPTGSTFLDSDRRRLTFERDSRGITT
                     ILLGKVPYTYLDSGRTLFAYRANRIQAIAGGYRWQNLDGKWEMYDTDGRLLKTGYRNL
                     LQTRRFYDAAGRLSEVRDATGAVLATYTYNAAGKVATIADRAGRQAEYEYEAGRLVRA
                     RDVTKRETRYEYGPDSGCGQRQLVTRVTRPDGTVQNFRYRSAGCDGPIFLYSRLDGTG
                     NGHFHEQHYDPNSRTYYLKITSTGGEVREKIFDSKGYLLEERVNGIVRRNILRSGRGD
                     LITDAAGNEKRRDYDEFGKVIREVAPDGGVTTWEYDPRYHLPTRIEVPRGAVTLMAYD
                     ENGNLTNKIEAAGTAIARTNSWVYNALNQMLRHVDGRGNAMNYEYDARGNLVREYDPS
                     NPAYQTLYAYDDRGNRVAITNALGHVTRFGYDLRDRVIAETNAVGHVTLYTYQDDNLI
                     EVETGRDGTTPGRIVRYRYDDHGRRTQTLRVDDQGQEHVWETTSYDGDGHVIATANAL
                     GQTTRYEYDANGR"
BASE COUNT         1669 a         3105 c         2708 g         1565 t
ORIGIN      
        1 gtgcgcctgg tcgctgtgaa tccagtcgcc acgcaaccct tcaacctcgg cgtggacttg
       61 atcgcccaag gcgacgagaa ctcggtcgga ttcagtttgc agttcgatgt cgctcgcctc
      121 gaatacatgg acgccacagc gggcagcggc ggtttcagcc tgctggtcaa caagagtcgg
      181 gcggcgcaag gccagctcgg attcgcactc ggtcgcagtg ctggaacggt ctttccgtca
      241 gggaccatcc gcatggtcaa cgtcgagttc cgtgcccgcg aaacgactgg aacgacgccg
      301 ctggcattcg cggatgtacc ggtcgtgcgg gagacggtgg gcgttcaggc tcagacactc
      361 acgacactat acgagggtct cagcctggag attctcccac tggtcgggcc ctcgattacc
      421 acccagcccg agggagccac cgttcatgcc gggaccaatt tcacgctgcg cgtcgtggcc
      481 gcaggcagcg aacctctgcg ctatcaatgg cagttgaacg gccaacccct ggcatccgcc
      541 accaacgcga cgctcgaact ttcctccatt cgcgaaagtc aagcgggcga ctatcgcgtc
      601 ggcgtatcca atccggcagg ttcggtcacc agcgcggtgg cccggttggt ggtcctgcct
      661 cccctgaccg ctccggtcat cgtgcggaat cccaaaccgg cattcgcatc ggcgggcgaa
      721 agagtcacgt tcacggtcga ggccacaggg agcgaggcgt tgtcctatca atggcaacgg
      781 caagggatcg acctggccgg cgcaacggag cctgtactcg tcattgacgc cgtcacgacc
      841 gcccatgctg gcaactatcg cgtgctggtc cgcaatccgg tcggctcggc cacgagcacc
      901 gccgtggctc tcactgtctc ggccacaccc cgcgcgatcc ggatcgggcg ggtcgattcc
      961 ccagccggca atcttatcga actgccgatc gatctctacg cgctgggcga cgaaaacgcc
     1021 gtcggattca gcctgacatt cgatcccgcg caactcgaat accgcggctg gcggcggggc
     1081 gcgaccgccc agggcacggc gctcaacgtc aacaccaacg gtctggccca gggccgcttg
     1141 ggcctggcta tcagcaaacc tgccggccaa cgcttcagca gcggcaccag cccgctcgtg
     1201 tatctgcaat tcctggtcgg tcgaacgcca tcccagacgg acattctggc cgctgacgcc
     1261 cctgtccggc aggaaatcgc cgatgtcggc ggtcgcagcc gccctgctga gttcgttgac
     1321 ggctgggtga acatcctcga caccccgcct tcgatcgtca gtgccccagc ggataccgtt
     1381 gcacggttgt tctcgaccgt gacactcgag gtcgtggcga acggcagtgc gccactgtac
     1441 tacgaatggc ggcacaacgg ccagccggtc cccggcgcca caagccccac gctgacgctc
     1501 gcttccgtga tgcccgatca agcgggagac tatcaagtgg tggtgagcaa cgcggtcgct
     1561 atcgccggca gtgacacggc gcgcctcgaa gtgcgccgcg tggttcgtct cgccggcacc
     1621 aacattccca ctggggtcac tgtcgacatg ccagtcgaac tcctgaccgc cggcaccgag
     1681 aacgccgtcg gattcagcct gcgctacgat ccgacgttgc tcacccttca atctgcgaag
     1741 ttgaatcccg ggttcacgaa cgtcacgctg aacctgcgca ccaacgcgag cttcccgggc
     1801 gcgctcggcg tagccttggc tcagccaccg ggcggtgtgt ttcccacagg gacccaggcc
     1861 gtgatgacgc tcaccttcca agccacccag ttgcccggca attgccccct gacgttcacg
     1921 gatatgccga tccttcggga gatggccaat gcagatgccc aatccgtgcc gacggacttt
     1981 gtggatggcg aaatcatcac cacccgtgtc gccccgcaga tcacccggca cccggtgccg
     2041 cgcgaggtaa cacagggcga atcggcggtg ttcgccgtgg tggcgagcgg ctcgttaccc
     2101 atgacttacc aatggctgca caacggtgtc gatctgcccg gcgcgaccaa caccacattc
     2161 agcatcgtcg ccgtccaacc cgttgacgcc ggcaactacg ccgttcgcct tagcaacgtc
     2221 gccagtaccg tcatcagctc gaatgcgctg ttgactgtga acatcccgga catgaccggc
     2281 ccggtgctgg ccaacccgac tttcgattct caaccgctgg cgccggacgc cctgctgacg
     2341 cgtgccggca ccctgcggat cgatgccacc gatccttcag gcgtcagccg ggtcgagttc
     2401 tatgtcgatg acctccaggt atcggccgat ctggacggcg cggatggatt cacggcgtat
     2461 ctcgatttcg agttgttccc ggacggtccg cacacgctgt cgtttcgggc gttggacttt
     2521 cgcagcaacc cggcggagtt cacgctgccg gtacggttcc tcctggccac actgccgccg
     2581 ccggtcatcg gcctgccgca gagcggcacg atcacctcga accagttcgt cacgattcgt
     2641 ggcaatgctc ccgcgcactc gagcgtggtt ctgtaccgca acgacgcgcg cgcgggcaat
     2701 cccgtgccgg tgaaccacct gggtatcttc gagacgacgc tgccgctgct tgaagggctc
     2761 aaccgtctcg aggccgcctc gctcaatcgc gccggtgaaa gccaacggtc gcccgaagtg
     2821 acggtgacac tcgacaccag cctgccggcg tcgccgtctg gactccgcgc tacggcgcgc
     2881 agcggcgggc gtgtcctgct cgagtgggtt ccgagcagcg agtcctaccg cggttaccac
     2941 gtttatcgct cgcatgcctc attcaccgat cgaagcgaag cggtcaggtt gaccggttcg
     3001 cctctgccag gctacacctt cgaggacacc gccccggtcg atggccagtg gttctatcgg
     3061 attgcgcagg tgaaccttgc caacgtcgag ggcctgctct cgatcgaggc ggctgtcgag
     3121 gtggaccgga cgccgcccac cgccgccctc gagtatcaac cgcccgcagc ttacgatccg
     3181 gccacgggcc gggtgggccg tggcatcgtt ggagtgctgg tgaacctgag tgaagaggtc
     3241 gccagcgcgc ctttcctgag cttcatgccg ccccaaggac agccgattgt catcgagttg
     3301 aggcgtgtcg atccgactca ataccgcggc aacttcgtga tcgatgacaa gaccccttcc
     3361 ggcaatgcag tcgccgtgtt ctccgcccgt gacaaagccg gcaaccgcgg cacccaaatc
     3421 acggcggggg cacgactgac gattgacacg gcagcgcccg agatcgtcgg tctgaccgtc
     3481 caacccgccg agccgatccg caacccggct gatgaaacgg tcctggtcac cctcgacctg
     3541 acattgagcg aggtcctgcg gacagccgcc gctcccgaaa tcacctactc gctgtccgag
     3601 tcagcgccga ccccgcagcc tgtggccagc ctgacgcctg gggcagaccc tgtccactgg
     3661 acggtgacgt tcaagctgcc tgccgccgcc ggggcaaacg ccgagaccct caccattcag
     3721 taccagggcg aagacgatct ggggaaccga ggctcgcgca tcatgccgcc ccatcagttt
     3781 caagtgtacc agggcgacct gcccgcgctg gtggcgcccg tgtccttgag cgccaaaacc
     3841 aagtcagcag gcctgattgt cctcgaatgg caacgggtga ccgacgcatc tgactacgcc
     3901 atctaccgga agacgcccag tgcgaccgag ttcgaaccct tgacctacac cggcgacacc
     3961 aatcgctggt ccgatctgcc gccggaagac ggcatctacg agtatgccgt cgcctccgtg
     4021 cgacgggtca acgcgcagga atcggtcagc gagctgagcg acgtggcgat tgccgagtcc
     4081 gaccgtcgtg cgcccggtgc gccctcggac ctcaccctgc aactggcgaa gaatggggtt
     4141 ttcctgcgct ggacatcgcc cgtgaacctc aacgaggaga tcacctattc gctctatcgc
     4201 acggtccaga ccccgccggc tgacctgacg gggctgacac cggcgatctc cggcattccc
     4261 atcgcccagg ttgtggaccc cgcgccgcgc cccgatcaac cgttctacgc cgtggccgcc
     4321 gttgaccgcg ccggcaatgt ctcgccgcca tcgaatcccg gctacctcaa catccagctc
     4381 tacccggtcc agtctttgac cgtcggccag acgaacaacg atccgcccat tctcacctgg
     4441 acccaggtcg gcagcggcgt ggccggacac gacatcttcc tcggcgacgc caactcgaac
     4501 ctgcacctga accggcgcgg tctgatcacc gacaaccgtt tcatcgacat cgggttcacc
     4561 ggcgaggagc ggctttacac catcttcacc gtggacaaca acggtcagcg cagtcccggc
     4621 cgcacgatcc tgctgccggc gttgagcgca gcgctcgatc cggacgccat cgtcaaacgc
     4681 ggcctgatga accgcttgac ctacaccgtg cgcaatgact cgaccgaccc gctcaacgag
     4741 gcgcgggtgc tcgtcacact cggcggacgc gtccacagct ccgagacctt ctggttggct
     4801 gggcagtcgg tcgtcgaaat ccccgtgatc gtaggcggat acgatgagtt gccagatggc
     4861 aatgcaccgc tcctcacgac gatcgagagc cgtcctgagg agggcgcgac ggtacgctgg
     4921 gcgcgcagcg ggtcggtagc ggttggcgat ggccagttgc tgatcggggt gttggcatct
     4981 gacctgtttc gcggggcgat cacgaaggct cgattcacac tgctcaaccc cagcactgag
     5041 gttctcgaag tgatcaccgc cacgcaacag ggcaaagcgc cctcgtcgga catccggatg
     5101 tcgcttttca acgccgaaag ccaactgctt tcctcgactc ccttcttcgc agcgatgggt
     5161 acgaatctgg ttctcttgcc caacggcaac atcgtgctgc gcctcgagcc gggccaggaa
     5221 gtcgtgtcgc aggagatcga tttgcccgtc ccgtcgagcg tgcccaacca tgtctggatt
     5281 cagctcgaga ttgaccgtgt cttctatcat agcgaccgcg aggaccgcgt cgagatgcgc
     5341 ggcgtggcga cggcggcacc gtttgtcgtg acctcgacca gttacaccgg cacgattacg
     5401 tcggtttcgc ccgagcactc gcggggcggc gagccgatca ccatttccgg acgcgcctgg
     5461 ttccaggcca gtcgccaacc ggcgccccgg caacccttgc tcgtcaagat cgccaatgcc
     5521 ggcttcgagc ggacggagca agtggtgacc gatgaccagg gcctgttcac cacccaattc
     5581 acccctctgg cgggcgaaag cggcggcatc tattccgtct gggccgtgca tcccgatctg
     5641 accgaccgca cggtgcagaa gcaatttgtt gtggatcggg tcctcgccag ccccaccgag
     5701 tttgtcgtgc gcgcgccgca ttactatcga caggttgtgc ccctcaaggt caccacaggt
     5761 gcagggaccg aagtcgcgaa tctgcgcctc gccttcttac ctcaagacca gcccggcaga
     5821 gcgttgccgc cgggcgtggc cgtggacacg ggcgcgccca ttgccagtct ggggccgaat
     5881 cagactgcct cgatgagctt tctcgtcact ggcacccccg aggccactcg caaggtcgac
     5941 ctggtgctcc gattagtcag cggcgaaacc gtcgataatc cctgggccac gattcgggcc
     6001 gcttgcgagt tctccgaggc ctacccctcg ctgcgttggt cgccctcact ggtggacacg
     6061 ggcgcgccac ccggctcgaa tgtggttgag gaggttacgt tcgagaacgt tggcctcgtc
     6121 tccgctacga atgtcttctt ctcgctgcac aaagccgacg gcaccgctgc gccttcctgg
     6181 gcggctctgg cgacaccgcc gaaccttgcc gagttggcgg tcggggccaa ggcgatcgtc
     6241 gggctgacgt tccgaccgcc gtctgccctg ccggaagacg attattggtt caagctcaag
     6301 acgcggtcag gcaaccatcc cacagtcgag atcggcgttc acgtcgccgt tctggccgag
     6361 ggacgcggca acctcgcgtt caaggtggta gacatgtaca ccggcacgcc cggcaacgat
     6421 ggcgtgtctg gcgccaatat ccgcctgcgc aacgacgcag tgggtagctt ccagacgaac
     6481 cttgtcaccg atatcaacgg cgaagcagtc ttcgaggacc ttcccagtgg gctctacgac
     6541 taccacgtca cgggcgagaa tcacaacagc agcaacggcc gggcgtgggt ccgcgccggc
     6601 agcacttcga gccagcacgt tgcgctcgcg tacagcctgg tcagtgtcga gtgggaggtt
     6661 gtgcccatca ccatcgaaga ccgttacgaa atcgttctca ccgccgtgtt cgagaccgat
     6721 gttccggccc ctgttgtcac catcgaaccg gcggcggtca acctgccgca actgttcgca
     6781 ggcgacgtgt tctacgggga gtacgtcatc gagaaccacg gcctgatccg ggcggataat
     6841 ctccagttcc aactgccggc gtccgacgcc tacctcaagt atgagattct cgccgccgtg
     6901 cccgaccggc tcgaagcgaa gcagaaggtg cgcatcccgt atcgcattac ctgtctcacg
     6961 tccttccccg gcccgcgcac tccgcccccc accgccaatg ctgctttggc caggacccgg
     7021 acgacggctc agcggccgtc tgccgtcaat cttgatggtt tgccgcccgc aatacgccaa
     7081 atcgtcctga tggacaaacc ttccggcatc tgcttcacgc acacgtccgg taccagcctc
     7141 atgtacgaat ggatctgcat caacggggag tggtaccgga atgcaatcgg aagtcagttc
     7201 tggtacaact attcgtccgg accttgtggc acgcctggcg gcggtgcgac ttgggtgggc
     7261 ggcggcggag ccacgttctc cggcccctcc gcctcgatac ccggactgcc tgagtgctgc
     7321 ccgcccgagg agtgcgaaga ggaagacccg tgtgacccca agtgttgtga agccgagggc
     7381 gcaacgtgcg acagtagccc gccgcctcct gggacgcccg gttcgccggg gtccgagggc
     7441 agtggcggcc ctgacgattc ggaccagcgg gttcgcttgg ctaatgctgg cggccaggct
     7501 ggagcccggt tcaaattcat aaaccaactc cgcgtcgagc cgacgggcag cacgttcctc
     7561 gattccgacc gccgccggct caccttcgag cgcgacagcc gcggcatcac gaccattctg
     7621 ctcggaaaag tcccttacac gtatcttgac agcgggcgca cgctgttcgc ctaccgcgcc
     7681 aaccgcatcc aggccatcgc gggcggttac cggtggcaaa acctcgatgg caaatgggag
     7741 atgtatgata ccgacggacg cctgctcaag accggctacc gaaatctact ccaaacccgc
     7801 cggttctatg atgccgcggg tcgcctgagc gaggtgcgcg atgccacggg ggccgtgctc
     7861 gccacctaca cctacaacgc agccggcaag gtcgccacga ttgccgatcg cgcgggtcgg
     7921 caggcagagt acgaatacga agccggacgg ctcgttcgcg cccgggacgt caccaaacgt
     7981 gagacgcgct acgagtacgg gccagattct gggtgcggcc agcgccagct cgtcacccgc
     8041 gtgacgcggc ccgacggaac tgtgcagaac tttcggtatc gcagtgccgg ctgtgacggc
     8101 ccgatattcc tctacagccg cttggatggc acgggtaatg gtcatttcca cgaacagcac
     8161 tacgacccca attctcgcac gtattacctg aagatcacca gcaccggcgg cgaggtgcgc
     8221 gaaaagatct tcgacagcaa gggctacctg ctcgaggagc gggtgaacgg catcgtcagg
     8281 cggaacatcc tccgcagcgg acgcggcgac ctcatcaccg atgccgcggg caatgagaag
     8341 cgccgcgact acgacgagtt cggcaaggta atccgcgagg tcgcgcctga cggcggggtc
     8401 acgacctggg agtacgaccc acggtatcac cttccgaccc ggatcgaggt cccgcgcggc
     8461 gccgtcacgc tgatggccta cgacgaaaac ggcaacctca ccaacaagat cgaagccgcc
     8521 ggcaccgcaa ttgcccgcac caactcctgg gtgtacaacg cgctcaacca gatgctccgc
     8581 cacgtggatg ggcgcggaaa cgcgatgaac tacgaatacg acgcccgtgg gaacctcgtc
     8641 cgcgaatacg atccgtccaa tccggcctat cagaccctgt acgcctacga cgaccgtggg
     8701 aaccgggtgg cgataacgaa cgccctcggc cacgtcaccc ggttcggcta tgacctccgc
     8761 gaccgggtga tcgccgagac caacgccgtc ggccacgtga cactgtacac ttaccaggac
     8821 gataatctca tcgaagtcga gaccggacgc gacggcacaa cgccgggccg gatcgtccgt
     8881 taccgctacg acgatcacgg tcgccgcacg cagacgcttc gcgtggacga ccagggccag
     8941 gaacacgtct gggaaaccac gagctacgac ggcgacggcc atgtgatcgc cactgccaat
     9001 gcgctcggcc agaccacccg gtacgaatac gacgccaacg gacgcca
//
LOCUS       DAOQRO010000114         9019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7156_length_9019_cov_4.374498, whole genome shotgun sequence.
ACCESSION   DAOQRO010000114 DAOQRO010000000
VERSION     DAOQRO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7156_length_9019_cov_4.374498"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PKK20_05905"
     CDS             <1..1017
                     /locus_tag="PKK20_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99452.1"
                     /translation="PFIESIALDEIERVDFAGGDIHRLAIRWRSGAQVWVNRSSDDWE
                     VAGHILPPFGFHATNGPVFSAIERLGGLIVERSMGAGTLYVNGRGYRPDAPLLIQPEF
                     AGFTPTGERQFRLQVNWQTQKPAPRDLQVFYHFSRPVPGRYSPAEFYGGGTPATPTSQ
                     WRDRESTDFTVQIPEDMEPGEYTAMVGLYNPRGEGGRRYPLLGDETSDRRYRVGKLIV
                     SGSSGKMSGLHFEPLSEPHRPDLRLVPNADPTDFGGIVTSGAVRVARQGGRLLLMPLP
                     EGEDLEVSLVPSRLLDRPVRPTTLKAVDANGARGRAIPFVQEGETIRFTLRRSEFGCE
                     LSVD"
     gene            1601..2425
                     /locus_tag="PKK20_05910"
     CDS             1601..2425
                     /locus_tag="PKK20_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99453.1"
                     /translation="MRTHSVSRRSGSRRESAFTLIELLVVIAIIAILAGLLLPALSKA
                     KQKGQGIACMNHTKQLGLAWIMYADENNDKLVINTTIDLTQASRTNNWVANVMGASPQ
                     EDTNTVYLTQGLLAPYVAKAIGIYRCPADRSLSGPLPDGRRLPRVRSVSMNAFVGPFD
                     KSNQPLISGWAQFIRMSDMPSPSSIFVLLDEHPNSINDGWYVFCNNGPDGNLWSDMPA
                     SYHNGAAGFAFADGHSEIKRWLAEVTKRARWSAWPIPTGADRRDFLWVSERATFRR"
     gene            complement(2509..3255)
                     /locus_tag="PKK20_05915"
     CDS             complement(2509..3255)
                     /locus_tag="PKK20_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99454.1"
                     /translation="MYLHNEAYAEFLAGWDEGFYAKYADTLRPDQAGDRALDVGCGVG
                     QVVRRLRDEGYEVCGVEVSEPNVAKARQAGLDCQVYDGRHLPFPDRHFGSVGALNVLE
                     HVEDPEGFLAELVRVARPGARVVVSSPNFFRALGWRDYHPRMRGLGNKWRNARRLLAK
                     RRQMRVAPGEVQFDRMTPIVKEPFTPDDDAIVATNGLEMTFFLDRLGCAIERVECTDR
                     YVRQPADYLLNLTPLRYAMFNAFVVARRRR"
     gene            3465..3851
                     /locus_tag="PKK20_05920"
     CDS             3465..3851
                     /locus_tag="PKK20_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNU99455.1"
                     /translation="MSQNTFHRLGDLQLRLMQSLWARGEASVAEVHATLPDDANLAYT
                     TVATMLRKMEARGLVRHRVEGRTFIYSAAVAEEQVSGGMAEHVLDRLFGGSVEALVSH
                     LLTRREVSRDELARLRKLIAERSHSS"
     gene            3848..6079
                     /locus_tag="PKK20_05925"
     CDS             3848..6079
                     /locus_tag="PKK20_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNU99456.1"
                     /translation="MSPLPTTGLWLQTLGLLAAQSTLLLVVASFSQGLARAPQSRRRF
                     WLAALAGVALLLANSLAGLDRPLARHFAAGAPSSLGVIVRGNLPVTSAPTGADASETS
                     LRSAAGLPSEAPPTQVGTCWPAWLWLAGAAAIGLWSLAPRIWLACVGRRAPAAESPEF
                     QRRIQSLAGRLNIRLRVRVVWMPRLAGPVAFGVVRPSIGLPADFWTAHSAIEQDAVLA
                     HELAHLAARDPFWLALADGLAAVLWWHPLVWWGRRQLRAACESAADEASLVVEDGPAV
                     LAGCLVALASRWQQRGVLGLLGMAGFRSGLGRRVERLLHLRGRDQPSLRCRWPGLVTS
                     LGAVATLALIVAVPARLFPVQAANRPALIEVVGDALAQTTVSPAASAPAARNGPSAAA
                     TDPAAASATPGDDAQVMIEAKFVEVSADANVPLSFDWYLGDVPSSDPGVPSASNSTGH
                     VATMTGILTDPQFREVIAASSKAGMNGVRELRGDQLPWPGRDATNAANIRVTAAQGAT
                     VTGILTDAQSRAVLRALEQRAGVDVLSAPSVVTVSGRQAQIQVADIRTVLTGINPDAV
                     KVPGAIPATNALPFTRTAVPTGPSLDVIPVVSSDGVTINLTVIPTVTEFLGYDEPPKD
                     SKVEIMENGKARLVELPLPRFRVRQMETQAAIRDGQTLVLGGFPVEETRHAKDKVPVL
                     GDIRLVGGLFRSESKSAVRKQLLVFVTATIIDSAGNRVNASPGTSAPAHTAPRFPGEA
                     PVP"
     gene            6467..>9019
                     /locus_tag="PKK20_05930"
     CDS             6467..>9019
                     /locus_tag="PKK20_05930"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNU99457.1"
                     /translation="MTEPHSLFGTLQTLDLGGGRTGQYYSLPALTQAGLGNLERLPIS
                     LRIVLESVLRNCDGKKITEQNVRDLAGWQPRAERTTEIPFVVARILLQDFTGVPLLVD
                     LAAMRSAAARLGKSPKTVEPLVPVDLVIDHSVQVDFAGTSDAARNNLEMEFMRNRERY
                     QFLKWSMQAFDQLKVVPPGIGIVHQVNLEHLAKGVQQSPATLNGESAPVLYPDTLVGT
                     DSHTTMINGLGVVAWGVGGIEAEAGMLGQPVYILTPDVVGVCLTGALREGVTATDLAL
                     TVTQLLRRANVVGKFVEFFGPGAAALSLVDRTTIANMAPEYGATMGFFPMDRETVDFL
                     RATGRSEDLCEACERYYRAQGLFGMPAPGQIDYSTEIALDLSSIVPSVAGPRRPQDRI
                     ELSALKQAFLDLLAKPASENGYGKSPEESARRLLVKGHRPINFHHEWHAGFNKARIEP
                     SESEMRDQHPAPDNITVSRDTLKCELGHGTILIAAITSCTNTSNPSVMLAAGLLAKKA
                     VERGLSVNPVVKTSLAPGSRVVTDYLEKTGLQPYLDRLGFHLVGYGCTTCIGNSGPLE
                     THIEAAVVHHDLIAASVLSGNRNFEARVHQSIKANFLMSPPLVVAFALAGRIDIDLTA
                     EPLGRDAEGRPVFLKDVWPRQEEIRDLLHSTLKPEVYRRLYSDFTTQNPRWNEIQSSG
                     GDVYQWDPTSGYIQEPPFFASFSRRPSAIAGIKGARVLGIFGDSVTTDHISPAGAIKK
                     DSPAGRYLLDHHVAIENFNSYGSRRGNDRVMTRGTFANVLIKNLMLGGEAGGDTLYQP
                     TGERMSIYDAAMRYAETKTPLIVIAGSEYGTGSSRDWAAKGPALLGVRAVVAR"
BASE COUNT         1578 a         3009 c         2837 g         1595 t
ORIGIN      
        1 cccttcatcg agagcatcgc attggatgaa atcgaacggg tcgatttcgc cggcggcgat
       61 attcatcgcc ttgcgatccg ctggagatcg ggcgcgcagg tctgggtcaa caggagctcc
      121 gacgactggg aggttgccgg acacatcctg ccgccattcg gattccatgc aaccaacgga
      181 ccggtctttt cggccatcga gcgcctgggc ggcctgattg tcgaacgctc gatgggcgcc
      241 ggaacgcttt atgtcaatgg ccggggttac cgcccggacg ccccgctcct catacagccc
      301 gaattcgcgg gcttcacgcc cactggagaa cggcaattcc gattgcaggt caattggcag
      361 actcagaagc ctgccccccg cgatcttcag gtcttctacc acttttcgcg ccccgtcccc
      421 gggcgctata gcccagccga attctatggg ggcggcactc ccgccacccc gacttcgcag
      481 tggcgcgacc gggagagcac cgacttcacg gtgcagatcc ccgaggacat ggagccgggc
      541 gaatacacgg cgatggtggg cctctataat ccccgaggcg aaggcggacg acgctatcct
      601 ctcctcgggg acgagacctc tgaccggcgg tatcgcgtgg gcaaactgat cgtttcgggc
      661 tcatcgggca agatgtccgg ccttcacttc gagccccttt cggagccgca tcggcccgac
      721 ttgcgcctgg tccccaacgc agacccgacc gatttcggcg ggattgtaac gtctggcgcg
      781 gtacgggtcg cgcggcaggg aggccgtttg ctgctgatgc cgttgcctga gggcgaggat
      841 ctcgaagtca gccttgtccc gagtcgtctc ctcgatcgcc cggtgcgccc gacgacgttg
      901 aaggccgtgg acgcgaacgg tgcccgtggc cgcgcgattc cgtttgtcca ggagggcgaa
      961 acgattcgat tcactcttcg ccgatcggaa ttcggatgtg agctgagtgt ggactgagga
     1021 ctggtcgttg gctgatgttg gccgcggcgg gggcgccatg ccttcgccca ggaagaagct
     1081 cgggtgcgga ggttggttgt accccacgtt ctgccaggcg atgctcagcc tgtaccgcgc
     1141 atcgagcatc aacgtgcgaa gcaggcttcg gcaggcaggt cggatgggag agggccgggg
     1201 tgagcggtct gtgtagggta tccagccgac taggcaataa cagtcaacgc cctgacaatt
     1261 gagggtcagt gcatctgtag cttgtcgatg agaataaagc ttgcgccgcg tggggcctgc
     1321 ctgtgccgtg cccgacacgg cagacaggcc gctgcgcttt gcggcgcagg cagggcacgc
     1381 ggcctacagc gggagtctcc atccgttgca ggcccggtgt cctcatcggg cgacagaata
     1441 ccgacaactg tcagatgcac cgattgcaag acccgccttt ctgaacttga gtgcccgcct
     1501 ccgttcgact agtcttgacc ggtgaaacac gttgcagaca agtcaagtct ctgtgcagcg
     1561 ttaaacgggt tgaggattct actcggaatg gcatgacatt atgcgcactc actcagtctc
     1621 gcgtcgctcg ggcagtcgtc gggagtccgc gttcaccttg attgaactcc tggttgtaat
     1681 cgccatcatc gcgatcctcg ccggcctcct gctgccggcg ctttcgaagg ccaaacagaa
     1741 gggccagggc atcgcctgca tgaaccacac caaacaactg gggttggcct ggatcatgta
     1801 tgccgatgag aacaacgaca aactggtcat caacaccacc atcgacctca cgcaagccag
     1861 tcgcacgaac aactgggtag cgaacgtgat gggggccagc ccgcaggaag acaccaatac
     1921 ggtttatctc acgcagggat tgctggcccc ctacgtcgcc aaggcgatcg gcatctaccg
     1981 ctgccccgcc gatcgcagtc tgagtggccc cctcccggat ggccggcgcc tgccgcgcgt
     2041 ccgcagcgtc tccatgaacg cgttcgtcgg gcctttcgac aaatccaacc aacccctgat
     2101 ctcgggttgg gcccagttca tccgaatgtc cgacatgccg agtccgtcga gcatcttcgt
     2161 cctcctcgat gagcatccca actcgatcaa cgacgggtgg tatgtattct gcaacaacgg
     2221 tcccgacggg aacctctgga gcgacatgcc agcgagctat cacaatgggg cggcgggttt
     2281 tgctttcgcc gacggacatt ccgagatcaa acgatggctg gcggaggtca caaagcgcgc
     2341 ccgttggtcc gcttggccga ttccgaccgg cgccgaccgc cgggacttcc tctgggtctc
     2401 cgaacgggcg acgttccggc gctagcccgc atagtcgtcc tcttgcgaag tctgaccgct
     2461 ccgtgcacgg acggggtcat ccgggacgaa ctcttcgatg gccccgcctc accttcgcct
     2521 gcgggccacg acgaacgcat tgaacatcgc gtaccgcagc ggggtgagat tgagcagata
     2581 atccgccggc tgccgcacat aacggtcggt gcactcgacg cgctcgatcg cacagccgag
     2641 acgatcaagg aagaatgtca tctccaggcc gttcgtggcc acgatcgcgt catcgtccgg
     2701 tgtgaacggt tccttcacga tcggcgtcat ccggtcgaat tgcacctcgc cgggcgcgac
     2761 gcgcatttgg cgccgtttgg ccagcaaacg ccgcgcattg cgccacttgt tgcccaaccc
     2821 gcgcatcctc ggatgatagt cgcgccagcc caacgcgcga aagaagttcg gactcgacac
     2881 aaccacccgg gcccctggac gcgccacacg caccaactcg gcgaggaatc cttccggatc
     2941 ctcgacatgc tcaagcacgt tcaaagcgcc cacggacccg aaatgccgat ccgggaacgg
     3001 caaatgccgt ccatcgtaaa cctggcaatc cagacccgct tgacgagcct ttgcgacgtt
     3061 cggttcggac acctcgacgc cgcagacctc ataaccctcg tcccgcagtc ggcgtaccac
     3121 ctgtccgacg ccgcacccga catccagtgc ccggtcgcca gcctgatccg gacgcaacgt
     3181 gtcggcgtat ttcgcgtaga agccctcgtc ccatcccgcg agaaactccg cgtacgcctc
     3241 gttgtgcaga tacatctctt ggtgacccat tcggattcca acaatgcacc atcccacttc
     3301 ccggcacaag aaccgcgacg caaccgaacc gcggcgccag gcaatgaggt ctccttgtcg
     3361 aggcagccac cctgcggcag gcggagtcga tcggaatcca actttggccc ttgacagaat
     3421 actaccacgg tagtactact gcggtcgtca aagcgacatc tcgtatgagt cagaacacat
     3481 ttcatcgtct cggcgacctc cagttgcgcc tcatgcagtc gctctgggct cgcggcgagg
     3541 ccagcgtcgc ggaggtccac gccaccctcc cggatgacgc gaatctggcc tacaccaccg
     3601 tcgccaccat gctgcgaaag atggaggcgc gcggacttgt ccggcaccgg gttgaaggcc
     3661 ggaccttcat ctactcggca gccgtcgccg aggagcaggt cagcggcggc atggccgagc
     3721 acgtgctcga tcgccttttc ggcggcagcg tcgaagcgct ggtcagccac ctgctgactc
     3781 gccgcgaagt gagccgcgac gaactcgctc gcctgcggaa gctgatcgcg gaaaggagcc
     3841 actcgtcatg agcccgctgc ccaccactgg actttggctg cagaccctgg gcctgctggc
     3901 cgcacagagc accttgttgt tggtcgtcgc cagcttcagt cagggcctcg cccgcgcgcc
     3961 gcagagccgc cgtcgcttct ggttggccgc gttggccggc gtggctttgc ttctggcaaa
     4021 ctcccttgca ggactcgacc gtcccctcgc caggcacttc gcggccggcg cgccttcctc
     4081 cctgggggtc atcgttcgtg gcaacttgcc agtgacgtcc gcaccgaccg gtgcggacgc
     4141 gagtgaaacc agccttcggt ccgccgcggg cttgcccagc gaggcgccgc caacccaggt
     4201 cggtacatgt tggccggcgt ggctctggct ggcgggcgct gccgccatcg ggctctggtc
     4261 gctcgcgccg cgaatctggc tggcttgtgt cggtcggcgc gcccccgccg ccgaatcgcc
     4321 ggagttccag cggcggatcc aaagcctcgc ggggcggctg aacatccgcc tccgtgtgcg
     4381 ggtggtctgg atgccccggc tcgccggccc ggtcgctttc ggcgtggtcc ggcccagcat
     4441 cgggttgccg gcggacttct ggaccgcgca ctcggccatc gagcaagacg ctgtgcttgc
     4501 gcacgaactc gcccaccttg ccgcgcgcga tccgttctgg ctcgcgctgg cggacggact
     4561 ggccgcggtg ctctggtggc atccgctggt ctggtggggg cggcggcagt tgcgcgccgc
     4621 ctgcgagtcg gcggctgatg aagcgagcct ggtggtcgag gacggtcccg cagtgttggc
     4681 cggctgcctg gtggcgctgg cctcgcgctg gcagcaacgc ggtgtgctcg ggttgctcgg
     4741 catggcagga ttccgttccg gcttggggcg gcgcgtggag aggttgctgc atcttcgcgg
     4801 gcgcgaccaa ccgtcgctcc gctgccgctg gccgggactc gtgacctcgc tgggcgccgt
     4861 ggcgactctc gcgctgattg tggccgtgcc cgcacggctc ttcccggtgc aggccgcaaa
     4921 ccggccagcg ctgatcgagg tcgttggcga cgcccttgcg cagacgacgg tctcgccagc
     4981 cgcgtctgct ccggcggccc gcaatggacc gagcgccgcc gctaccgatc ccgccgccgc
     5041 cagcgccaca cctggggacg atgcgcaggt gatgatcgag gccaagttcg ttgaggtttc
     5101 ggcggatgcg aatgtgccgc tcagtttcga ctggtatctt ggcgatgtcc cgtcctcgga
     5161 tccgggagtg ccgtccgcaa gcaactcgac cggccacgtt gcaacgatga ccggcatcct
     5221 caccgatccc cagttccgcg aagtgatcgc agcgtcgtcg aaggcgggca tgaacggcgt
     5281 gcgcgagttg cgcggcgacc aactaccctg gccgggccgc gacgcgacca acgcggccaa
     5341 catccgcgtc accgccgcac aaggcgccac cgtgactggc atcctcaccg acgcgcaatc
     5401 tcgcgcggtg ttgcgcgcgc tcgaacagcg cgccggcgtg gacgtgctct ccgcgccgag
     5461 cgtggtcacc gtgagcggac gccaggcgca gattcaggtc gcggacatcc ggaccgtgct
     5521 gaccggcata aaccccgatg ccgtgaaggt ccccggtgcc atcccggcca ccaatgcgct
     5581 gccgtttacc agaaccgccg ttccgaccgg ccccagcctc gatgtgatcc cggtggtctc
     5641 gtccgacggc gtgacgatca acctgacggt catccccacc gtcaccgagt tcctgggcta
     5701 tgacgaaccg cccaaggact ccaaagtgga aatcatggag aacggcaagg cgcgcctcgt
     5761 ggagctgccg ctgccgcgct tccgcgtgcg ccagatggag acacaggcgg cgattcgcga
     5821 cggccagaca ctggtgctgg gcggttttcc ggtcgaggag acgcgtcacg cgaaggacaa
     5881 ggtgccggtg ctgggcgaca tccggctggt cgggggactg ttccgaagtg aatcgaagtc
     5941 cgcagtgcga aaacagttgc tggtcttcgt gacggctaca atcatcgact cggcggggaa
     6001 ccgggtgaac gcatcgcccg gaacgtcggc gcccgcccat accgcgccgc ggtttccagg
     6061 cgaggccccg gtcccgtaat ccgaacaggg gatccccttc ctgacctctt cactgaccca
     6121 cgcgattctg agagcccgca tttcaggtga aatgtgacgg tgaccggtca gatgacatca
     6181 ccagttggcg agaaatgcgg gctaaaagga actcctgctg cggccagaat caggcagccc
     6241 caccgagcca agccaaaacc ccaggggagt gacggcaacg ccactccctt gcccacatga
     6301 gggtgtcgca cattccacgg attcccctgt ctgtcgacac accgcggggg gttggcgggg
     6361 tcggcactcg aacactcggc aagcccgcct cgccagcttg acatcgcatc cgtcaatctt
     6421 cattttcttg ttttaagctc acgagaatcc gactgaccaa catggtatga ctgaaccaca
     6481 ttctttgttc gggacgcttc agaccctgga cctgggcggc ggccggaccg ggcagtacta
     6541 ttcgctgccg gcgctgacac aggcggggct gggcaatctg gagcggctcc cgatttcgct
     6601 gcgcattgtg ctcgaatccg tcctcaggaa ctgcgacgga aagaagatta ccgaacagaa
     6661 cgtgcgtgat ttggccgggt ggcagcctcg agccgagcgc acgaccgaga ttccgttcgt
     6721 cgtcgcccgc attctgctgc aggacttcac gggcgtgccg ttgctggtgg accttgcggc
     6781 catgcgttca gccgccgcgc ggctcgggaa gagtccgaag accgtcgagc cgctggtgcc
     6841 ggtggatttg gtgatcgacc attcggtgca ggtggacttt gccggaacca gcgacgccgc
     6901 ccgcaacaat ctcgagatgg agttcatgcg caaccgcgag cgctatcaat tcctgaaatg
     6961 gagcatgcag gcgttcgacc aactcaaggt cgtccccccg gggatcggga ttgtgcatca
     7021 ggtcaacctc gagcacctgg ccaagggtgt gcagcaatcg cctgcgaccc tgaacggaga
     7081 atccgcgccc gtcctttacc ccgacaccct ggtcggcacc gactcgcaca ccaccatgat
     7141 caacggcctg ggcgtcgttg cctggggcgt ggggggcatc gaggcagagg cgggcatgct
     7201 tggccagcct gtctacatcc tgacgccgga tgtggtgggc gtgtgcctga ccggcgcgct
     7261 ccgggaagga gtgacggcga cagacctggc gctcaccgtc acgcagctcc tgcgacgcgc
     7321 gaatgtggtg ggcaagttcg tcgagttctt cggacccggc gccgcggcgc tgtccctggt
     7381 ggatcggacc acaatcgcca acatggcgcc cgagtacggg gccacgatgg gctttttccc
     7441 gatggatcgg gagacagtgg actttctccg cgcgaccggc cggagcgagg atctctgtga
     7501 ggcgtgcgaa cgctactatc gggcccaggg gctttttggc atgccggcgc ccggccagat
     7561 cgactactcg accgagatcg cactcgacct ctcgagcatc gtaccaagcg tcgccggccc
     7621 caggcgcccc caggatcgca tcgagctgtc cgccctcaaa caggcattcc tcgacctgct
     7681 cgcgaagccg gcgtccgaga atgggtacgg caagtcgccc gaggaatccg cccgtcgcct
     7741 cctggtcaag ggccaccggc cgatcaactt ccatcacgag tggcacgccg gcttcaacaa
     7801 ggcccgcatc gagccaagcg agagcgagat gcgcgaccag catcccgcgc ccgacaacat
     7861 cactgtgagc cgcgacacgc tcaagtgcga gcttgggcac gggacgatcc tgatcgccgc
     7921 gatcaccagt tgcacgaaca cgtcgaaccc gagcgtgatg ctggcggcgg ggctgctcgc
     7981 gaagaaggcc gtcgagcgcg gcctgtccgt caatccggtc gtcaagacct ccctggcgcc
     8041 agggtcgcgg gtcgtgacgg actatctcga gaagaccgga ttgcagccgt atctggaccg
     8101 gctcggcttt catctggtgg gctacggttg caccacgtgc atcggcaatt cgggcccgct
     8161 cgagacgcac atcgaggcgg cggtggtgca tcacgatctc attgccgcct ccgtgctctc
     8221 tggcaatcgc aatttcgagg ctcgcgtcca ccagagcatc aaagccaatt tcctcatgtc
     8281 ccccccgctt gtcgtcgcgt tcgcccttgc cgggcggatc gacattgacc tgaccgctga
     8341 accgctgggc cgcgacgccg aggggcgccc ggtgtttctg aaagacgtct ggccgcggca
     8401 ggaggagatt cgcgatctgc tccactcgac tctcaagccc gaggtctacc gccgcctgta
     8461 ctcggatttc acgacccaga acccgcgatg gaacgagatt cagtcgagtg gaggcgacgt
     8521 ctaccagtgg gaccccacct cgggctacat ccaggaaccg ccgttcttcg cctcattctc
     8581 gcgccgcccg agcgccatcg cggggatcaa gggggcgcgc gtcctgggca tcttcggcga
     8641 cagcgtcacc acggaccaca tttcgccggc cggagcgatc aagaaggact cgcccgcggg
     8701 caggtacctg ctcgatcacc acgtagcgat cgagaacttt aacagttacg gctcgcggcg
     8761 cggcaatgac cgcgtcatga cgcgcggcac cttcgccaat gtgctcatca agaacctcat
     8821 gctcggtggc gaggccggcg gcgacacgct ctatcagccc acgggcgaga ggatgtcgat
     8881 ctacgatgcg gccatgagat atgccgagac caagacgcct ttgatcgtca tcgccggcag
     8941 cgaatacggc accggcagct cgcgcgattg ggcggccaag ggcccggctc tcctcggcgt
     9001 cagggcggtc gtggcccgc
//
LOCUS       DAOQRO010000115         9010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7166_length_9010_cov_4.456505, whole genome shotgun sequence.
ACCESSION   DAOQRO010000115 DAOQRO010000000
VERSION     DAOQRO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7166_length_9010_cov_4.456505"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1631)
                     /locus_tag="PKK20_05935"
     CDS             complement(132..1631)
                     /locus_tag="PKK20_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99458.1"
                     /translation="MFNPRLFDGDVYANQINVPAIGRYPQGSSTPEVVLVSVEHRTIA
                     PFRGANRTRYILGASSAATTVFSRYDFDFQNRIEAPVPEDQNTETFDWVDENTIIYCI
                     YNPSGNRKRLALATVNAEPFTVTPDTRWNEQGYITTSITGRIRNVRVGDVYSGYAYYG
                     DGGQNENPKFFAINLATGAETELGNAGTLTGTGSFGLWTVVERGGYLYVQTTDNGIRV
                     YEMTSATTLGALVTTYTQEQLSALTGYNGQFWGFDVTAEGNMIVSGTGLICELAGPAR
                     LQAYVLKPDWNIPPLAYDWMTTNHLQRGLAYNPGSGHVLTVSRYPANGSGQVYVIDSA
                     NGSVTGTLKTNGIKYDMNFPLNMIGAGDDGAIYACNLAVDSATQPAEPMSNNGPFRIY
                     RWADESADPVLVYEGDPSEGDATAAALKAACKAVRSSATWTALTTRLERGPGSDHPYG
                     CRPKRMRSRSMRRRSSATCTARIGVPGTIGSSVKRSCGTNPQNGPTGPS"
     gene            2111..3235
                     /locus_tag="PKK20_05940"
     CDS             2111..3235
                     /locus_tag="PKK20_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99459.1"
                     /translation="MNTRHLFQALRDMVSPALVLIAAVAVTGANRPPRETVGGQTARP
                     RDARDHEAAGRAMEGIRLGIERERFTLDGRPAFLLGISYYGGLGAGIDTLRKDLDSLK
                     SHGFNWIRLWATWSYAGHDVSAVDVQGRPREPYMTRLKTLVAECDRRGMVVDVTLSRG
                     RAAVGGGIPDMQAHRTAVQSIIGALGERANWYLDLANERDVRDARFVSTEEVRELRDL
                     ARRLAPGLAVTASFGGHDLGEAYVREALFEAGLDFVAPHRPRSAGTAAKTLSHTRMCL
                     AAMRQMGRLAPVHYQEPFRRGYGHWEPIAADYLTDLRGAVEGGAAGWCLHNGSRRGEK
                     NDRPRRSFDLREQSLFEQLDDEEMAVVRQAIEVVQDVWTR"
     gene            complement(3427..5862)
                     /locus_tag="PKK20_05945"
     CDS             complement(3427..5862)
                     /locus_tag="PKK20_05945"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99460.1"
                     /translation="MNAIEYWKQWMRITGLTLAGLSLACCCPPSAESGEGPVRLTVNT
                     DSPTHEINPNVYGHFFEHIYHSANGGLWGELVWNRSFELANAGMGNWSTENGEVVQSA
                     LVTDAHVLFGDRSWEDYELTLQALKERGTEGFLVLFRAEDDDSFYWLNLGGWNNTRHA
                     IEKEVEGRRRGIGRSRNGNIQTGRWYDVRIRCQGDHIQCWLDGEQIIDLRDEDNPYRK
                     GMIGLGTWGTHARYRNIKVTRLDNSDVLFSGLPTLPSKPFEFSFWRHFGSGKADKVED
                     ALNNDYSIRLTGDDAPTGIEQANFRFTPQRYYGSVWMKGALPGGIKIELLDHDTVLGE
                     AVLGPPRAGWAEFPFQIDSSGGTLDGSLRITLLGAGAVKLDQVSLMGEDALANGGFRP
                     DLLDAVRGLRPPIIRWPGGCFASVYLWKDAVGPQHERRIYSAYMWEDQDINSLGTDEY
                     MMLCNRVGAKPLLVINTGILDAGCGAPAQFKLASRDDYLPYALEWMEYCNGDASTPMG
                     ALRAAHGHPEPYGVVYWELDNETWGAGIEAYIATVLEFAPAMRAKAADLGTPIKIIAC
                     GGSSFDMRWNRGLIDACAPMIDYISVHHYENPQNFASGIERYEKLLLELADSIAASAN
                     PQMEIYNSEWNAQSTDWRTGLYAGGLLNAYERQGRKFTLGGPALFLRHLSAGAWDNAF
                     INFDHTGWFPAPNYVVMKLWWDNFAPGFLPVDGDPQGCNVVATRAEDGRTVVVKAINP
                     NAEERNVEVDLHGEFTPKTASMQVVAPGSLRARNTLEAPTAVRVETGMVQLTGQRVRF
                     ELPAYSAAVVRLN"
     gene            6055..6822
                     /locus_tag="PKK20_05950"
     CDS             6055..6822
                     /locus_tag="PKK20_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99461.1"
                     /translation="MPPRVEKILRELAAPSDPGVVILKSSSRRSVLRVKNFSPEIPSI
                     VVKGFPLKKIESRLKWKKYGLAEFQNYQQAASRGIPVPKCFGCFEVRAFFLVNANGTL
                     IEDLRGFRNLDELARENPGRRSTVLLAAVPLLKLLFETGVNHIDPSPHNMMQPPDGGG
                     LRLLDWQYCSFVAPRQPAQLALHAAHFLNFARCEINSDDWSRWLGELHAACGCNSSRE
                     RFQQAVAVLQSRGKITGDERLQLRLDSISHELLNQAL"
     gene            7003..8190
                     /locus_tag="PKK20_05955"
     CDS             7003..8190
                     /locus_tag="PKK20_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99462.1"
                     /translation="MNPTHFFRHQLLKLKWRGVRKRGHTLRLRIGNAPKFFAALNAAD
                     IRYVVLRWFDEVPLGDASTHNRDVDLLAESARLADIVRIAASCPGGIKCDLYTNTGKL
                     GTAYKRFPYYPPVLAEEILSARESFSETFFVPAPALHFKSLAYHLVYHKGAASGIPTG
                     TEIPTDAHSRRSYQYFIEMLGEKLGVGLKRPYTLLALHEHLKSIEWSMPHDLMVRWPQ
                     QHDWMHRLARHEEKPFEAFACALPDVIVFLLRADAAETAMEARALELLRSRFRVLKME
                     PLDAAQIRRVMHSVRGGNWVEHREGILIEPRVAWVCHDPQPQPVPENDPLRRKYPLVQ
                     NQNVFFKNEIRDRLNELFPAQPKRIAIHGSDNAMETQHYLHAIYGGRHAEICDELERL
                     CAR"
     gene            complement(8145..8804)
                     /locus_tag="PKK20_05960"
     CDS             complement(8145..8804)
                     /locus_tag="PKK20_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99463.1"
                     /translation="MRHQAFLIFTLTRANADRLSWLDESADQQEALAVEVHSLCSFGA
                     ASVNLSQAISDSREFRVASWLLALLPEWRGRGGPEIALDVIDRRRVEASHWLRQTNDA
                     ACAATAPFADLVPTLIVEPDGCLTPFIYGFPRHWAVGHLGRLADGADSARGASIPEDG
                     RASPRARKSGLLSAFTLSSDRNWRPQTVALQSAEQKRVEQKETEFHRAQSRSSSSHIS
                     A"
     gene            complement(8801..>9010)
                     /locus_tag="PKK20_05965"
     CDS             complement(8801..>9010)
                     /locus_tag="PKK20_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99464.1"
                     /translation="TEVRRGADLKIAWSSIEGRIYTVEHTAALPGGWASIATVPSHGS
                     ATSYTDTDPTRLGKAVGFYRVQLQP"
BASE COUNT         1675 a         2798 c         2771 g         1766 t
ORIGIN      
        1 ccagcgcagc actgtggcgt ggcgagcgag tcgaatcagc agttggtgat cgccggcacc
       61 attgcaaagc tgaatcagct gggcctgtca gggtcagcct tcgaagcgtt ccgggaagtc
      121 tggaaggcga gtcacgaagg gccggtgggg ccgttctggg gattcgtgcc gcacgacctc
      181 ttcaccgacg agccgatggt tccggggacg ccgattcggg ctgtgcacgt cgcagagctc
      241 cgtctgcgca tcgacctgct gcgcatccgc ttcggtctgc agccatacgg gtggtcggat
      301 ccaggccctc tctccagcct cgtcgtcagg gccgtccacg tcgccgaact tcgcactgcc
      361 ttgcaggcgg ccttcagcgc tgctgcggtg gcatcgccct cgctcggatc gccttcataa
      421 acgagaaccg ggtcggcgga ttcatcggcc catcgatata tccggaaggg cccattgttg
      481 ctcataggct cggcgggttg ggtcgcgctg tcgaccgcca ggttgcaggc gtagattgcc
      541 ccgtcatccc ccgcgccgat catattgagc gggaagttca tatcgtactt gatcccattg
      601 gttttcaggg ttccggtgac actcccgttg gcggaatcga tcacatagac ctgtccgctg
      661 ccattggcgg gataccggct gacggtgagg acatggccag aacccggatt ataagccagc
      721 ccgcgctgca ggtggttggt ggtcatccaa tcgtaggcca ggggcggaat gttccaatcg
      781 ggtttcagca cgtacgcttg gaggcgcgcc gggccggcca attcgcagat caagccggtt
      841 ccactcacga tcatgttgcc ctcggcggtc acatcgaatc cccagaactg gccattgtag
      901 ccggtgagcg cggacaattg ttcctgtgtg tacgtcgtca ccagtgcgcc cagagtcgtc
      961 gcactggtca tttcgtagac tcgaatgccg ttgtcggtgg tctggacgta gaggtaaccg
     1021 ccgcgttcaa ccacggtcca gaggccgaaa cttcccgtcc cggtcaacgt tcccgcgttg
     1081 cccagttcgg tttccgctcc cgttgccagg ttgatggcga agaacttcgg gttttcgttc
     1141 tggcccccat cgccgtagta agcgtagccg ctgtaaacat cgcccacacg gacattgcga
     1201 atccggccgg tgatgctggt cgtgatgtat ccttgctcgt tccatcgggt gtctggcgtc
     1261 accgtaaacg gctccgcatt caccgtcgcc agtgccaacc gcttgcggtt gcccgaagga
     1321 ttgtagatgc agtaaatgat cgtattctca tccacccagt caaaggtctc ggtattctgg
     1381 tcttcgggca ccggagcttc gatccggttc tggaagtcaa agtcataccg cgaaaacacc
     1441 gtggttgccg cgctgctggc gcccaggatg taacgagtcc gattggcgcc gcggaaaggc
     1501 gcgatggtgc ggtgctcgac cgacaccaac accacttccg gcgtgctgga accttgggga
     1561 taacgtccga tggcagggac gttgatttgg ttggcataga cgtccccgtc gaataaacga
     1621 gggttgaaca tgctgttggg agcaatccca aggtccgcat gaagctgaat ggtcgttcgc
     1681 agcctcagat tctcggatag aacggggtcc gcctggcatt gagaggcaaa ccatattgta
     1741 gcgacaagta atggtatttc taatcgtttt atgttcatga tcagttgcat tttagctttc
     1801 taccgcaaac cgcgccgatt ctaatgcaat caggggggat gggcaagcca ttccgttatt
     1861 ttgttgaacc caaaacccac aacgcactgc ccggcatgct ccgcgaggag gcgtagggcc
     1921 gctgagggcc aaggccaggc gccaggggac cgagaccgaa agacgccggc gaccgaatgc
     1981 ggggtccgca gattcggcgc atcccgatcc tcgactttgc gtcggcctca gctagcctgc
     2041 gccacgggca catgaactgg acagaacagt caactttctg ttccaatgcc agagtgcctt
     2101 cagaatcctc atgaacactc ggcatctctt tcaggctctg cgagacatgg tgtccccggc
     2161 attagtcctc attgcggctg tcgcagtcac cggcgcgaat cgccctcccc gcgaaaccgt
     2221 gggcggccag acggcgcgac cgcgggatgc ccgcgatcat gaggccgcag gccgagcgat
     2281 ggaaggcatc cgactcggca tcgagaggga gcggttcacc ctcgatgggc ggcctgcgtt
     2341 tctattgggc atcagttact acgggggcct gggagccggc atcgacacgc tgcgcaagga
     2401 tctcgattcc ttgaaatcgc atggcttcaa ctggatccgc ctctgggcaa cctggtccta
     2461 tgcgggacac gacgtttccg cggtcgatgt ccagggtcgt ccgcgcgagc cgtatatgac
     2521 acgcctcaag accctcgtgg ccgaatgcga caggcgcgga atggttgttg acgtcacctt
     2581 gagccgcggc cgcgccgccg tcggcggggg cattcccgat atgcaggcgc accggaccgc
     2641 ggtgcagtcg atcatcggcg ccctgggtga gagggccaat tggtatcttg acctggccaa
     2701 cgagcgggat gttcgggacg cccggtttgt ctcgacggag gaggtgcggg aattgcgcga
     2761 cctggcacgg cggttggcgc ctggcctggc ggtgacggcc tcatttggag ggcatgacct
     2821 gggggaagcc tatgtgcgcg aggcgctctt cgaggccggg ctcgatttcg tcgcgccaca
     2881 ccgccctcga tcggcgggaa ccgccgccaa aacgctgtcg cacacgagaa tgtgcctggc
     2941 cgccatgcgc cagatgggac gactggcgcc ggtccactac caggaaccgt tcaggcgcgg
     3001 atacggacac tgggaaccga ttgccgcgga ttacctgacc gacttgcgcg gcgcagtcga
     3061 gggcggcgcg gcggggtggt gcctgcacaa tggcagccgg cggggcgaga aaaacgaccg
     3121 cccccgcagg tcgttcgact tgcgcgagca atcgctgttc gagcaactcg acgatgagga
     3181 gatggccgtg gttcgccagg cgatcgaagt tgttcaagac gtctggacac gctgagatcg
     3241 gttccgtgcc tgaagaagtc accggcggtg tgtcccgtcc cgccgccgga actagccccg
     3301 gccgggttca ccacggctca atcggttgca tcgagagatc tcgccggcct cccatgtcca
     3361 aggtgcgtcc cggctgaact gtgggcgcgc aaacggtctt cctggatagg aaggaggcga
     3421 ggcaactcag ttgagcctca ccacggcagc cgaataggcc ggcaattcga agcgaacccg
     3481 ttgaccggtc agttgaacca tgccggtctc cacccgcacg gccgtaggcg cctcgagggt
     3541 gttgcgggcg cgcagcgagc cgggcgcgac cacctgcatc gacgcggtct tcggagtgaa
     3601 ttcgccgtgc aagtcgactt cgacgtttct ttcctcggcg ttggggttga tcgccttgac
     3661 aaccacggtt cggccgtcct cggcgcgggt tgcgacgacg ttgcatcctt gcggatcgcc
     3721 gtcgaccggg aggaagccgg gagcgaagtt atcccaccag agtttcatca cgacgtagtt
     3781 cggggccggg aaccagccgg tgtgatcgaa gttaatgaac gcgttgtccc aggcgccggc
     3841 ggagaggtgg cgcaggaaca gcgccggacc gccgagggtg aatttccggc cctgacgctc
     3901 ataggcattg agcaggccgc ccgcatacag gccggtgcgc caatcggtgg attgcgcgtt
     3961 ccattcggag ttgtagatct ccatctgcgg gttggcggag gcggcgatgg aatcggcaag
     4021 ctcgagcaac aacttctcat agcgctcgat ccccgaagcg aagttctgag gattctcgta
     4081 gtggtggacg ctgatatagt cgatcatggg ggcgcaggca tcgatcagcc cgcggttcca
     4141 gcgcatgtcg aagctgctgc cgccacaggc gatgatcttg atgggagtgc cgagatcggc
     4201 cgccttggcc cgcattgcag gcgcaaattc gagcaccgtc gcgatatagg cctcgatacc
     4261 ggcgccccaa gtctcgttgt cgagttccca gtaaaccaca ccgtatggct cgggatgccc
     4321 gtgggcggcg cggagggccc ccatgggggt cgacgcgtcg ccgttgcagt attccatcca
     4381 ttcgagggcg tagggcaggt aatcgtcgcg ggaggctagt ttgaactgag ccggcgcgcc
     4441 gcagccggca tcgaggatgc cggtattgat gacgagcaac ggcttcgcgc cgactcgatt
     4501 gcagagcatc atgtactcat cggtgcccaa cgagttgata tcctggtcct cccacatgta
     4561 ggccgagtag atgcgccgtt cgtgctgcgg ccccacggcg tccttccaca gatagaccga
     4621 cgcgaaacag cctcccggcc agcggatgat gggcggccgc aggccgcgca ccgcgtccag
     4681 cagatcgggt cggaacccgc cgttggccag ggcgtcttca cccatcaggc tcacctgatc
     4741 gagtttgacg gcgcccgcac ccagcagcgt gatgcgcagg gaaccgtcca gagtgccgcc
     4801 gctggagtcg atctgaaacg ggaactcggc ccaaccggcg cgaggcgggc ccaggacggc
     4861 ctcgcccaac acagtgtcgt ggtctagcaa ctcgatcttg atgcctccgg gcagcgcgcc
     4921 tttcatccag accgacccgt aatagcgctg tggcgtgaaa cggaaattcg cctgctcgat
     4981 gcccgtgggt gcatcgtcgc cggtgaggcg gatgctgtag tcgttattga gcgcgtcttc
     5041 gaccttgtcg gcctttccag agccgaagtg cctccagaaa ctgaactcga atggctttga
     5101 agggagcgtg ggcaatcccg agaaaaggac gtctgagttg tcgagtcgcg tcaccttgat
     5161 attgcggtag cgcgcgtgcg tgccccatgt gccaaggccg atcatgccct tccgatacgg
     5221 gttgtcctcg tcccgcagat cgatgatctg ttctccatcg agccagcatt ggatatggtc
     5281 gccttgacag cgaatgcgga cgtcgtacca acggcctgtc tggatgttgc catttcggct
     5341 ccgtccgatt ccacggcgtc tgccctcaac ttccttctcg atggcgtggc gggtattgtt
     5401 ccagccgccc aggttcagcc agtagaagga gtcatcgtcc tccgctcgaa acagcaccag
     5461 gaagccctcg gttccgcgct ccttgagggc ctgcagtgtc agttcataat cctcccagga
     5521 tctgtcgccg aacaggacat gggcgtctgt gaccagcgcc gactggacca cttcgccatt
     5581 ttcggtcgac cagttgccca tcccggcatt ggccaactcg aaactccggt tccagaccag
     5641 ctcgccccag agaccgccat tggcggagtg atagatgtgc tcgaagaaat ggccgtagac
     5701 attcggattg atttcgtgtg ttgggctgtc ggtgttcacc gtcagccgga ccggcccctc
     5761 cccggattcc gcgcttggcg ggcagcagca cgccagggac aatcctgcga gggtcaggcc
     5821 agtgatcctc atccattgct tccaatactc gatcgcgttc atagtcttga tcccaaatga
     5881 ctcgtccggc cgcggctctc cgtccccgcc acagaatccg gaaacgaact gtgtaattgc
     5941 tcttgtcgcg ccggtaaagt taatcattct caacgcgtga tgaaacaccc cgagccgttc
     6001 acagagcagc gccgcgccgg cggcctccgg ctctggctgt cccccggctt cgacgtgccg
     6061 ccgcgcgtgg aaaaaatcct gcgcgaactg gccgccccat ccgatcccgg cgtggtaatc
     6121 ctgaaatcgt ccagccgccg cagcgttttg cgcgtaaaga atttctcgcc ggagattccg
     6181 tccatagtgg tcaaaggttt tccactgaaa aagatcgagt ccaggctcaa atggaaaaag
     6241 tacgggctgg cggagtttca gaattaccag caagccgcct cgcgcggaat ccccgtgccc
     6301 aagtgtttcg gttgctttga agtgcgggcg ttttttctcg tcaacgcgaa cggaacgctc
     6361 atcgaagacc tgcgcggttt ccggaacctt gacgagctgg cgcgcgaaaa tccggggcgg
     6421 agatcgacgg tgttgctcgc ggcggttccg ctgctcaaac ttctcttcga gaccggcgtg
     6481 aaccacattg accccagccc acacaacatg atgcagccgc ccgacggcgg aggtttgcgg
     6541 cttttagact ggcaatactg ctcgtttgtc gcgccgcgcc agccagcgca actcgcgctc
     6601 cacgccgcgc atttcctgaa cttcgcccgc tgcgaaatca actcggacga ctggagccgt
     6661 tggctcggcg aacttcacgc cgcctgcggg tgcaactcct cgcgcgaaag atttcagcaa
     6721 gccgtcgccg tcttgcagtc gcgcggaaaa atcaccggcg acgagcggct tcagttaaga
     6781 ctcgattcga taagccacga attgcttaat caggctttat gatgaaagga attttcgcca
     6841 ggcagtggcc acatcgttgt gaggttctgg agtgcggtgg gtgagggatg aaggcacaca
     6901 ccgctttggc tctggcgcca tgccggcaaa ccaaagcggc gtcagccccg ttcccatcca
     6961 gccaccgcac tcccgaacgc ttgtgcgcgc ttgtttgctc tgatgaatcc cacccatttt
     7021 tttcgccacc agcttctcaa gctgaagtgg cggggcgtcc gcaaacgcgg gcacacgttg
     7081 cggttgcgca tcggcaacgc gccaaagttt ttcgccgcgc tgaacgccgc ggacatccgc
     7141 tacgtggtgc tccgctggtt cgatgaagtt cctctgggcg acgcttcgac acacaaccgg
     7201 gatgttgacc tgcttgccga atcagcccgg ctcgccgaca tcgtgcgcat cgccgcttcg
     7261 tgtcccggcg gcatcaaatg cgacctttac accaacaccg gcaagctcgg cacggcctac
     7321 aaaaggtttc cctactaccc gcccgtgctc gcggaggaaa tcctgtccgc gcgcgaatcg
     7381 ttttcagaga ctttcttcgt gcccgcgccc gcgctgcatt tcaagtcgct ggcctaccac
     7441 ctcgtctatc acaaaggcgc ggcgtctgga attcccacgg gcacagaaat cccgaccgac
     7501 gcgcactcgc gccgctcgta tcaatatttc atcgagatgc tcggcgaaaa actcggcgtc
     7561 gggctcaagc gcccctacac gctgctcgcg ctgcacgaac atctgaaatc gatcgagtgg
     7621 tcaatgccgc acgacctgat ggtgcgctgg ccgcagcaac atgactggat gcaccggctc
     7681 gcgcgccacg aggaaaaacc gtttgaagct ttcgcgtgcg ccctgccgga cgtgattgtt
     7741 tttttgttgc gcgccgacgc cgcagaaacc gcgatggaag caagggcgct ggaacttttg
     7801 cgatcgcgct tccgcgtttt gaaaatggag ccgctcgatg ccgcgcaaat ccgtcgcgtg
     7861 atgcattccg tgcgcggcgg gaactgggtg gagcatcgcg aggggattct catcgagccg
     7921 cgcgtggcgt gggtttgcca cgacccacaa ccgcagccag tccctgaaaa cgacccgttg
     7981 cgtcgcaaat acccgctcgt gcagaatcaa aacgtgtttt tcaagaacga aatccgcgac
     8041 cggctgaacg aacttttccc ggcgcagcca aaacgcatcg ccatccacgg cagcgacaac
     8101 gcgatggaga cgcagcatta tctgcacgcg atctacggcg ggcgtcacgc ggagatatgc
     8161 gacgaactgg aacgactttg cgcccgatga aactcggttt ccttttgttc gactcttttc
     8221 tgttcggcgg attgcagcgc gactgtctgg ggtctccaat tcctatcaga cgacagggta
     8281 aacgctgaaa ggaggccgga tttcctcgcg cgcggcgagg ccctgccatc ctccgggatg
     8341 gatgctccgc gggcgctgtc agcgccatcc gccaaccgac cgagatgtcc caccgcccag
     8401 tgtcgcggaa acccgtagat gaacggcgtc agacacccgt cgggctcgac gatcaaggtg
     8461 gggaccagat cggcgaaggg cgccgtggcg gcgcacgccg cgtcgttggt ctgccgcagc
     8521 cagtgacttg cctccacccg gcggcggtcg atcacgtcca acgcgatttc cggtcctccc
     8581 ctgccccgcc actcgggcaa caaggccagc aaccaactcg ccacgcggaa ctcccggctg
     8641 tcggaaattg cctggctaag gttgacggag gcggccccga aactgcacaa ggaatgcact
     8701 tcgacagcca gggcctcttg ttggtcggca gactcgtcga gccaactgag ccggtcggca
     8761 ttggcgcgcg tgagcgtgaa gatcaggaat gcctggtgcc tcacggctgc aactgaactc
     8821 gatagaaccc gacggccttt cccaggcgcg tcgggtcggt gtcggtgtag cttgttgcgc
     8881 ttccgtggct cggcaccgta gcgatgctcg cccaaccgcc gggcagggcc gcagtgtgct
     8941 cgacggtgta tatccggccc tcgatgctgc tccacgcaat cttgaggtcc gcaccccgcc
     9001 gcacctcggt
//
LOCUS       DAOQRO010000116         9009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7170_length_9009_cov_4.535515, whole genome shotgun sequence.
ACCESSION   DAOQRO010000116 DAOQRO010000000
VERSION     DAOQRO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7170_length_9009_cov_4.535515"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKK20_05970"
     CDS             complement(<1..264)
                     /locus_tag="PKK20_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNU99465.1"
                     /translation="MQQVNVGMIGGGTVGGGVFQALERNGALLSSRAGVRVHLRKIAV
                     KAFDEPRKVDIPRALLTLDWREVVNDPEVSIVIELAGGTTALDP"
     gene            complement(335..1681)
                     /locus_tag="PKK20_05975"
     CDS             complement(335..1681)
                     /locus_tag="PKK20_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99466.1"
                     /translation="MCRWIGLVVLAAVLGQVRAATVYSRSGQFVIHSPYATMPTLNQL
                     PTPVSSNRVTLAPDPLAVSCERIKTGVLRELDLPDHWRGKVHVRIKPRLRAGATPGFV
                     STRYADGWQYTLELPEELEPDALVRSVVLAVLSEIANRQVGVRPAEIPIWLAEGLSAS
                     AAHAIGPDLVVAPTPLRARLGASVGQLDTTPQTRVGAENLGEVRAWLFDRVPLTFAQL
                     SQPAPDFLSGESGTTYRHCAHLLVRHLQALPQGRRGLQTFVFSLTRSLNWQTAFLEAF
                     SGQFSRLLDVEKWWTVSMQGFLQGDQPNAWSRAVSLDKLGDILGVRVEVQDRPRAPRR
                     ATVLSLQEFMGKTEFAVHRAVVRVRVSRLQAIQRSAAREIVPLIESYRAVLDEYLSHR
                     AQAGRSPLAKRVGSLPPALLLREATRQLDELDRRRAQARLLADRPLAPLSDSGQRR"
     gene            complement(1797..2246)
                     /locus_tag="PKK20_05980"
     CDS             complement(1797..2246)
                     /locus_tag="PKK20_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNU99467.1"
                     /translation="MKQGLVELGIPEGAPRAQTHPASRVANSVVFGKGKRPAHSPVGS
                     MPTYHYMCEKCGHEFDHFQSMSDSPLKTCLKEKCGRKTWGKGRVKRVLSGGAGLIFKG
                     SGFYITDYRSEGYKTAAKKESEVAAPKKDASSTAKKETKSEKPASST"
     gene            complement(2309..4825)
                     /locus_tag="PKK20_05985"
     CDS             complement(2309..4825)
                     /locus_tag="PKK20_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HNU99468.1"
                     /translation="MNSLFERSEDRRMRKTMLFRFSGLLACVCLCAWVLLCRGAETVA
                     SLSTSPAGEPTPDIRLRLLIETDAGGDPDDEQSLVRFLLCANEWDVEGIIANRPEARA
                     GENRNEERTGLGIVRRLLEAYRQCHTNLARHDPRYPAPETLWARTVPGYDDTDEGVRL
                     ILAAVDSADPRPLWYSDWGSDRGSATNNLRRALDRVLRERGPDGYAAFKSRLRLVSYD
                     AFGEHTAGLEPPFSLWVNTFAPEVSGRRWYHRFAALTATAGGFDLARDVLTGHGPLGA
                     LYPTNSGPRQKEGDTMTFLYLFPTGMNDPERPHWGSWAERYGANKEHAGKPYYWANCQ
                     DAWRGATNRDNTLGRWAEALQNDFRARLDWCVRLPAEANHPPQVRVDSPFHRIVRSGE
                     SIRLSAAASGDPDGDALTFDWVAYPETGTYRGVVPSRAEGPLLAFETPRVTSPETVHW
                     IVAVTDRGSPPLTRYRRVVCTVLPRAAEPADDGSWQKVKSAFEPPGTHADGRATLKSL
                     FRFDNGREVQTAADWAERRAELLESWHAIMGPWPPLLEHPRLDVLGTETRENLEQRRV
                     RLEIAAGQTGEGWLLLPAGRGPFPAVLVVYYEPETSVGLNPEQPLRDFGLELARRGFV
                     TLSLGTPGGNAWKPELGAAQCQPLSYHAYVAANAWRALASLAEVDPERIGVVGHSYGG
                     KWALFAGALWDRFAAVAVSDPGIVWDESRPNVNYWEPWYLGRDATRTRQPGVPTEANP
                     RTGAYARLVAQGRDLHEVHALIAPRPFLVSGGSEDPPERWRALNHTVAVNRLLGVTNR
                     VGMTNRATHTPTRESNEQIYAFFEVFLRAQPTGDACGRGR"
     gene            complement(4822..6822)
                     /locus_tag="PKK20_05990"
     CDS             complement(4822..6822)
                     /locus_tag="PKK20_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99469.1"
                     /translation="MFRLASRLVLGCLLASARAGEGVHFEGRYFAGHGDRDYVHLLDI
                     ARDMFDASARQPNLALLYVTNWNGLVEGPTWDAWWIQNSYGTTYAALPFLQEPFVTFL
                     QNSQDLWFDQMGDGKRVGAAPPFDWVAPDGALCDAARPGWIVYKQGDGRTAIHDWGME
                     FTAAGMVLQGELLLIGRDIEAIAKYLPKLERCARFIESRRDPTNDLFLAGPAGNLLAP
                     SYAGWKKPDGTFGQAYLTGLSVTYIAALDRLIELEMLAKRMDEATSWAERRDRAKRGL
                     QRLAMPEGYFIRSQDPDGTRHGVYGAERHGYFEASPNHDAIAFRVVDDAQAERIYQTI
                     ASIPGLRPHQFVLPNYPSYDDMYERPEGLWAYGTWVNGGHWSTCEARMILAYYRLGKH
                     EDARRSMSQLMHFADGFRMDNPLTKCGSDVYQPALPINLTYDAFGPPAAFVRGLFEYR
                     YSAEGLTLVPHIPDGLSRIDQLFPIRFGRRQVFVSAAGRGRITDVLVDGRRWERHDAA
                     TVFLPYESMPEVAKVRIALGGAKIESPSAESPFPPPLVALPSGHECVVCQEIGRSGAR
                     LAQFHRKASEAGLGDRYEAAHARLALACAWAAHERVELVKKGQVPRLPEVSEAAARKA
                     YAASARQLYDGLVRVLEASADGKSGIEQRLAALWRETATGAR"
     gene            7495..8406
                     /locus_tag="PKK20_05995"
     CDS             7495..8406
                     /locus_tag="PKK20_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNU99470.1"
                     /translation="MKPPTPLVTVSPKKRFRWFGPLCLVVFLVAFILQYRQTEQLRRT
                     MDELRLAEQTRALNAVTEDSEVANLRRQVLQLRDALTRLQTVATNQSDQPGGPVPSPG
                     DTRSEAVDWVRWAALPGSQVIIEGTSSIHDWTVKGGIIGGFFEAEPALLTEHPVPSAW
                     TNQAVARVQVKIPVRSLRSQTLIGASKMDEIMQEAMRMKENPDILYRLRSMKAKEAPS
                     GQEAVVRFDTVGEVTVAGVTNAIDIEVQMCRLETNRLRFSGVKSLKMTDFKIPPPSPK
                     LPGMDFIKTGDEVTIRFEWLTGCRVSP"
     gene            8552..>9009
                     /locus_tag="PKK20_06000"
     CDS             8552..>9009
                     /locus_tag="PKK20_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99471.1"
                     /translation="MKRNFPAAILCFMTLTFASSPRAAAASFGDDVAFLKKHVDVFEL
                     SDKKGAAKAAVSAAYQGRVMTSTAGSDAGLSFGWINRELIASGKYLPHMNAFGGEDRF
                     WLGPEGGQFSIFFAKGAKFELADWFTPAAIDTLPFEVVRASRDRARFAAK"
BASE COUNT         1684 a         3115 c         2675 g         1535 t
ORIGIN      
        1 cggatcgagc gccgtcgtgc ccccggcaag ctcgatcacg atgctcacct cggggtcatt
       61 gaccacctcg cgccagtcca acgtcagcag cgcgcgcggg atgtcgacct tgcgcggctc
      121 gtcgaacgct ttcaccgcga tcttgcgcag atgcacccgc acccccgccc tcgaactcaa
      181 cagggcgccg tttcgttcga gtgcctgaaa cacaccgcca ccaacggtcc caccgccaat
      241 catgcccaca ttcacttgct gcataaagcg ggtgagattg ggcgagaccc gcccggacga
      301 caaccccctt tttcaagtcg tgcgcagcgg ctcattaacg cctctgcccg ctgtccgaga
      361 gcggcgccag cggccggtcc gcaagcagcc tcgcctgcgc gcgccgccga tccagttcgt
      421 ccaactggcg cgtcgcctcg cgaagcagca acgccggcgg cagggatccg acgcgcttgg
      481 ccaagggaga tcgaccggcc tgcgcgcggt ggctgaggta ctcgtcgagc acggcccggt
      541 agctctcgat cagaggcacg atctcgcgtg ccgcgctgcg ctgaatcgcc tgcagtcggc
      601 tgactcgaac tcggacaacg gctcgatgca cggcgaattc cgtcttgccc atgaactcct
      661 gcagcgacag cacggtcgcg cgccgcgggg cgcgcggacg gtcctggacc tcgacacgaa
      721 cgccaagaat gtcgcccagc ttgtcgagac tgaccgcacg agaccatgcg ttgggctgat
      781 cgccctgcag gaagccctgc atgctgaccg tccaccactt ctcgacatcc agcagccggc
      841 tgaactgccc cgagaaggcc tcgagaaacg ccgtctgcca attcagcgac cgcgtcaggg
      901 aaaagacgaa cgtctgcaga ccgcggcgcc cttggggcaa ggcctggaga tgccgcacaa
      961 gcaaatgcgc gcagtgacga tacgtcgtcc cgctctcgcc cgacagaaaa tccggcgccg
     1021 gctgactcag ttgcgcgaat gtgagaggaa cacgatcgaa cagccaggcc cgcacctccc
     1081 ccagattctc agcacccacc cgagtctgcg gcgtcgtgtc aagctgcccc accgaagctc
     1141 ccagccgcgc gcgcagtgga gtcggcgcca ccaccaggtc cggaccgatc gcatgggctg
     1201 cactggcgga caagccttcg gccaaccaga tcggaatctc cgcgggccgc acccccacct
     1261 ggcgattggc tatctccgac aacaccgcaa gcacgaccga gcgcaccaga gcgtccggct
     1321 cgagctcctc cggcaactcg agcgtgtact gccagccatc cgcataccgc gtcgacacaa
     1381 acccaggcgt cgcgccggcg cgaagccgag gcttgatccg gacatgcact ttgccgcgcc
     1441 agtgatcagg caaatccaac tcccgcagca ctccggtctt gatgcgctcg cacgagaccg
     1501 ccagaggatc gggcgccaac gtcacccggt tcgaactgac cggggtgggc agttgattca
     1561 gggtgggcat ggtggcgtac ggactgtgaa tcacaaactg gcccgagcgg ctgtacactg
     1621 tcgcggcccg cacctgcccc aaaaccgccg ccaataccac cagcccaatc caacggcaca
     1681 tccgcgcccg gcttgcgccg aacagaggga ccgagccgac ggggccaggg cggacccttc
     1741 ctcggcccgc accggctgct tgccatgtct cgaaggtccg atcgcaggcc gacgactcag
     1801 gttgaactcg ccggcttttc agacttcgtc tccttctttg cagtggaaga cgcatctttc
     1861 ttgggggccg caacctcgga ctccttcttt gcagccgtct tgtatccttc actccgatag
     1921 tcagtgatgt agaaaccgct gcccttgaaa atcagtccgg cgccgccgct cagaacgcgc
     1981 ttcacccgcc ccttgcccca tgtctttcgt ccgcacttct ctttcagaca cgtcttgagg
     2041 ggcgaatcgg acatcgattg aaaatggtcg aactcgtgac cgcacttctc acacatgtaa
     2101 tgatatgttg gcatagaacc gaccggcgaa tgcgccgggc gcttcccttt accaaaaacc
     2161 accgagtttg caactctgct cgcggggtgt gtctgcgcgc gcggtgcccc ttccggaatg
     2221 ccgagttcca cgaggccctg tttcatgacg gtgattgtct cgagtcagga actctgtcgc
     2281 agccgcgaga tcgtccctcc gaatcggttc atcgtcctcg tccgcatgca tcgccggtag
     2341 gttgggcccg gagaaagacc tcgaagaacg cgtaaatctg ctcgttcgat tcccgcgtgg
     2401 gcgtgtgcgt cgcgcggttc gtcatcccaa cccggttggt aacgccaagc aggcgattga
     2461 cggcaacggt gtgattgagc gcgcgccagc gctcgggcgg gtcctcggat ccgcccgaaa
     2521 ccaggaaagg ccggggcgcg atcaacgcat gcacctcgtg caggtcccgg ccctgcgcca
     2581 ccaaccgcgc gtaagcgcct gtccgcggat tcgcctcggt cgggacgcca ggctggcgcg
     2641 tgcgggtcgc atcgcggccc aggtaccatg gctcccagta gttcacattg ggccggctct
     2701 cgtcccagac aataccggga tcgctcactg ccaccgcggc gaaccgatcc cacaatgcgc
     2761 ccgcaaacaa cgcccatttg cccccgtaac tgtggccgac aaccccaatc cgctcagggt
     2821 cgacctcggc aagactcgcg agcgcgcgcc aggcattggc cgccacgtac gcatgatagg
     2881 aaagcggctg gcattgcgcc gcccctaatt ccggcttcca tgcattgccc ccaggcgtcc
     2941 ccaggctcag agtcacaaat ccgcgccggg caagctcgag gccaaagtcc cgcagcggtt
     3001 gttcgggatt caaccctacg ctcgtctcag gctcgtagta caccacgagc accgccggga
     3061 atggtccgcg tcccgccggc agcagcagcc agccttcacc ggtctggccg gccgcgatct
     3121 cgagccgcac acggcgttgt tcaagattct cccgtgtctc ggtgccaagc acatccagcc
     3181 gcgggtgctc gagcaacggc ggccacggcc ccatgatcgc atgccaggat tcgagcagtt
     3241 cggcgcgccg ctcggcccaa tcggcggcgg tttgcacttc acgtccgttg tcgaagcgga
     3301 acagtgattt caaggtcgcc cggccgtccg catgcgtgcc gggcggctcg aatgcggact
     3361 tgaccttctg ccaggacccg tcgtcagccg gctcggcggc gcgcggcaac acagtgcaga
     3421 caacacgccg gtaacgcgtc aacggcggag aaccccgatc cgtgacggcg acgatccaat
     3481 gcaccgtttc cggcgaggtc actcgaggcg tctcgaatgc cagcaaaggc ccctccgccc
     3541 gcgaagggac gactccacga tacgtgccgg tctccggata agccacccag tcgaacgtca
     3601 gtgcatcgcc atccggatcg cccgacgccg cggcgctcag gcggatgctc tccccgctgc
     3661 gcacgatccg gtggaaggga ctatcgacac gcacctgggg cgggtgattc gcctctgcgg
     3721 gcaatcgaac gcaccagtcc agtcgcgcgc ggaagtcgtt ctgcagagcc tcagcccaac
     3781 gtccgagcgt gttatcgcga ttggtcgcgc cccgccatgc atcctggcaa ttcgcccagt
     3841 aatagggctt cccggcatgc tccttgttcg caccatagcg ttccgcccaa ctgccccagt
     3901 gcgggcgctc cgggtcgttc atccccgtcg gaaaaaggta gaggaaagtc atcgtgtcac
     3961 cttccttttg acggggccca ctgtttgtcg ggtagagcgc gcccagcggt ccatggccgg
     4021 tcaggacgtc ccgggcaagg tcgaatccgc ccgctgtggc cgtcagggcg gcgaatcggt
     4081 ggtaccagcg gcggcccgag acctcgggcg caaacgtgtt gacccaaagc gaaaaaggcg
     4141 gttcgagccc ggcggtatgc tcgccgaacg catcgtacga cacgagccgc aaacgcgact
     4201 taaacgccgc gtacccgtcc ggcccgcgtt cccgcaggac tcgatccaaa gcgcgccgca
     4261 ggttgttcgt ggcgctcccg cggtcgctgc cccagtccga gtaccacagc ggacgcggat
     4321 cagcggaatc gaccgcggca agaatcaagc gaaccccctc gtccgtgtcg tcatagcccg
     4381 gcacggtcct cgcccaaagg gtttctgggg ccggatagcg cgggtcgtgc cgtgcgagat
     4441 tcgtatgaca ctggcgatag gcttcgagca agcggcggac aatgcccagg ccggtccgtt
     4501 cctcgttgcg gttctcgccg gcgcgcgcct ccgggcggtt ggcaatgatg ccttcgacat
     4561 cccactcgtt ggcgcagagc aggaatcgaa cgagcgattg ttcgtcgtcc ggatcgccgc
     4621 cggcatcggt ctcgatcagc agacgcagcc gaatgtccgg tgtcggttcg ccggcaggcg
     4681 atgtggaaag gctggccacg gtctcagccc cccgacacag caagacccac gcgcacagac
     4741 agacgcacgc cagcaagcca gaaaaccgga acagcatggt ctttcgcatc cgccgatcct
     4801 cggaccgctc gaacaacgag ttcatctggc gcccgtcgcc gtctcgcgcc acaaggccgc
     4861 aagccgttgc tcgattccac tcttcccgtc cgcggacgct tcgagcacac ggaccaaccc
     4921 gtcgtacaac tggcgcgcgc tcgcggcgta ggccttccgg gccgctgcct cggacacctc
     4981 cggcaaccgc ggcacctggc ctttcttgac caactcgacg cgctcgtgcg ccgcccaggc
     5041 gcaggccagc gccaaacgcg cgtgcgccgc ctcgtaccgg tcgcccaggc cggcctcgga
     5101 tgcctttcgg tgaaactgag ccaatcgcgc gccggagcgc ccgatctcct ggcacacgac
     5161 gcactcgtgt ccggacggca gagcgaccag tggcggcggg aagggcgact cggcggacgg
     5221 cgattcgatc ttcgccccgc ccaacgcaat ccggaccttc gccacttcag gcatcgactc
     5281 gtaaggcaag aacaccgtcg ccgcatcgtg acgctcccat cgccgcccgt cgaccagaac
     5341 gtccgtgatt cggccccggc cggcggctga gacaaagacc tgtctgcgcc cgaagcggat
     5401 cgggaacaac tgatcgatgc gggagaggcc gtccgggatg tgcggaacca gcgtgaggcc
     5461 ctcggcgctg taacgatact cgaagaggcc ccgcacaaag gccgcgggcg ggccgaaggc
     5521 atcgtaggtg agattgatcg gaagcgccgg ctggtagacg tcgctgccgc acttggtgag
     5581 cggattgtcc atccggaaac cgtccgcgaa atgcatcaac tggctcatcg agcggcgggc
     5641 gtcctcgtgt tttccaagac ggtagtaggc cagtatcatg cgcgcctcgc aggtcgacca
     5701 atgcccgccg ttcacccacg tgccgtaggc ccaaagtccc tcaggacgct cgtacatgtc
     5761 gtcgtaagac gggtagttgg gcagcacgaa ttggtgcgga cgcaaccccg gaatcgatgc
     5821 gatggtctgg tagattcgtt ccgcctgcgc gtcgtccaca actcgaaacg cgatcgcgtc
     5881 gtggttgggc gaggcctcga agtacccgtg tcgttccgcg ccgtacaccc cgtgcctcgt
     5941 tccgtcggga tcctgcgacc ggatgaaata tccctcgggc atggcgagcc gctgcagacc
     6001 ccgcttggcc cggtcccgcc tctcggccca ggaagtcgcc tcgtccatgc gcttggccag
     6061 catctccaac tcgatcaatc gatccaacgc cgcgatatac gtgacggaaa gacccgtgag
     6121 gtaggcctga ccgaaggtgc cgtccggctt cttccagccc gcatagcttg gcgccagaag
     6181 attgccggct ggcccggcaa ggaacagatc gttggttgga tcccggcgcg actcgatgaa
     6241 acgcgcgcag cgttcgagct tgggcagata cttcgcaatc gcctcgatgt cgcgcccgat
     6301 cagcagcagc tcgccttgca ataccatccc cgccgccgtg aactccatcc cccaatcgtg
     6361 aatggccgtc cgcccgtcgc cttgcttgta cacgatccat cccggacgcg ccgcatcgca
     6421 caaagcgccg tcgggcgcca cccagtcgaa tggcggcgcc gctccgacgc gtttgccgtc
     6481 acccatctga tcgaaccata gatcctgcga attctgaagg aacgtaacga acggttcctg
     6541 caggaacggc agcgcggcgt aggtggtgcc gtagctgttc tgtatccacc acgcatccca
     6601 cgtcggcccc tcgaccagtc cgttccagtt cgtcacgtag agcagcgcca ggttcggctg
     6661 gcgcgcactg gcatcgaaca tgtcccgcgc gatgtcgaga aggtgcacat aatcgcgatc
     6721 cccatgcccg gcaaaatacc ggccctcgaa atgcacgccc tccccggcgc gcgccgatgc
     6781 gagcagacag cccagaacga gccggctcgc aagtcggaac atgccggctt tcccaatcaa
     6841 tgtcccaccc atgcgcaggg ccgagttcca caaagccctg cagggtgacg gcgattgtct
     6901 caagacaaag actcgccgag cccgcccctc caattctcgc ccctccaaga ccgattcatg
     6961 gtccccgtgc gcattcagcc gttgagtgtc gagacccgcc tggggtgtct cggatttgga
     7021 gacctttgcg ccggaccacc cagcccgcgc cgtttcggtt gattcagtca tgccatgtcc
     7081 tctgtgttcg atgatgcacc caggcacagc cgccgactcc tcgtgcggtc cgcacatcaa
     7141 tcgccggatt caaaacactg actcgcgcct cgccaagaca cgcgaagtgg attgaatcga
     7201 cgtcctgcgc gacaagaacc agggccatca tcgggcaatg cgagaactct gcgcttgaac
     7261 tcctcgccaa agcaactctc gaaactctcc cgaaaactcg gggttcggca ttgacgcatg
     7321 ccgatggcag ggtttgagtt gcgtccatgc ccgctccggt tcagacactc gagtccgaag
     7381 gccgcctcgg aggttgtcga agacccgtct ggacctcacg cgccgtcgtg ccgccctccc
     7441 gattcggacc ctgggcgttc aggccatcgc tcctctgcca acacagcgtg atctatgaaa
     7501 cctcccacac ccctcgtgac cgtgtcgccg aagaagcgct ttcgctggtt cggcccgctt
     7561 tgcctcgtgg tgttcttggt ggcattcatc ttgcagtacc gccagaccga gcaactccgc
     7621 cggacgatgg acgaattgcg cctggccgag cagacgcgcg ccctcaacgc cgtcaccgag
     7681 gattcagagg tggcaaatct tcgtcgccag gtcctccaac tccgcgatgc gttgacccgc
     7741 ttgcagaccg tcgcgacgaa ccaatccgac cagcccggcg gtcccgttcc cagtccgggc
     7801 gacacccgat ctgaggctgt cgactgggtt cgctgggcgg ccctgccggg cagccaggtg
     7861 atcatcgaag gcacctcgag catccacgac tggacggtga aaggcggcat tatcggcggc
     7921 ttcttcgagg ccgagcccgc gcttctcacg gagcatccgg ttccctccgc ctggaccaac
     7981 caagccgtcg cgcgggttca ggtcaaaatc cccgttcgat ccctccggag ccagacgctc
     8041 atcggggcgt ccaagatgga cgagatcatg caggaggcga tgcggatgaa ggagaatccg
     8101 gacatcctct atcgacttcg ctcgatgaag gccaaagaag cgccgtccgg gcaggaggca
     8161 gtcgtgagat tcgacaccgt cggcgaagtg accgtggcgg gggtcaccaa cgccatcgac
     8221 atcgaggtgc agatgtgccg tctcgagacc aatcgcctca ggttcagcgg cgtcaagtcg
     8281 ctcaagatga ccgacttcaa gatcccgccc ccctcaccga aacttccggg catggacttc
     8341 atcaagaccg gtgacgaggt cacgatccgg ttcgaatggc ttacaggctg cagggtcagc
     8401 ccttaaggtt taagtttggt gacgagtaat tgatggtcgt gtcccgcaag gtcacactga
     8461 aagaagatcg gccgacgatt aatttaaatc ttaagggctg accccgctgc ggacttgacc
     8521 tttcacgcgc ctcacccgta gctttcaggc catgaaacgc aacttcccgg cagccattct
     8581 ctgtttcatg accttaacat tcgcctcctc tccccgcgcc gccgccgcct cgttcggcga
     8641 cgacgtcgcc ttcctgaaga aacacgttga tgtcttcgaa ctcagcgaca agaaaggagc
     8701 ggccaaggcc gcagtgtccg cggcttacca gggccgcgtg atgacgagca cagcaggaag
     8761 cgacgccggc ctgagcttcg gctggatcaa tcgagagttg attgcgtcgg gcaagtacct
     8821 gccgcacatg aatgcgttcg gcggagaaga ccgtttctgg ctggggccgg agggggggca
     8881 gttctcgatc ttctttgcca aaggagcgaa gttcgagctg gcggactggt tcacgccggc
     8941 ggccatcgac accctgccct ttgaggtggt ccgagcatcg cgggatcggg cgcgattcgc
     9001 cgccaagtt
//
LOCUS       DAOQRO010000117         8995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7187_length_8995_cov_4.685347, whole genome shotgun sequence.
ACCESSION   DAOQRO010000117 DAOQRO010000000
VERSION     DAOQRO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_8995_cov_4.685347"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..986
                     /locus_tag="PKK20_06005"
     CDS             450..986
                     /locus_tag="PKK20_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99472.1"
                     /translation="MLKRVVLTGAVTLLCLNVGQALAQQGGGGMGGGRGNFDPEQMRQ
                     RMMERYKEAFGVTDNSEWKIIEERINKVNEARREIGFGGMGMMGMGRRPGQGGDQARP
                     DQDRQRRMGPQPDPAAEELQKAIEDKASNDVLKAKMTALRDSVKAKEAKLQAAQEDLR
                     KVLSVRQEALALQMGLVR"
     gene            1063..2811
                     /locus_tag="PKK20_06010"
     CDS             1063..2811
                     /locus_tag="PKK20_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNU99473.1"
                     /translation="MVVSETETTALRELLDRMVAAKQLSPVDAQIIASQPAGEDRTLV
                     RSEEDVLRWLGEEYDVPFTPLDEVEPDKQVLSLFPARILLRDGLLPLRRVNGCIEVAT
                     SRLFATQGLDTLRTMTGLRLKAVLAPSEAIQREMKKRLGVGADTIDTLDEETSFQVVE
                     EGDEEDTNLDTAAEDASIIRFVNQVLRDAIELRASDIHLEPFEDELRIRYRIDGVLQE
                     VPVPAQVKRFQPAIVSRVKILSHLNIAEKRLPQDGRIKIRVDESEVDIRVSVIPMLHG
                     EAVVLRLLRQSAMLRGMQELGMDAHEARSFRRVLGLPHGIVLVTGPTGSGKTSTLYTA
                     LQEINDSVRKIITIEDPIEYQLRGVNQIQVSEKAGLTFARGLRSILRHDPDVILVGEI
                     RDQETAQIAVQASLTGHLVFSTLHTNDAPGALTRLVDMGVEPYLVASSLEAVVAQRLV
                     RVLCTQCKQPDTSETAQALKAQLGFPPDTIVYRPVGCGVCRNTGYHGRRAIFEWMDTN
                     QEIRRMVLNNRSSDEIREAARRGGMRTLAEDGWRLVRLGVTPPEEVLRVTKEQSPAGE
                     IEVDAPAAPAPAEAAA"
     gene            2969..4231
                     /locus_tag="PKK20_06015"
     CDS             2969..4231
                     /locus_tag="PKK20_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU99474.1"
                     /translation="MPTYSYRAITPSGTITEGQIEAGGRHDAFRQMEGRALRPISLTE
                     LAGGPNGKPNGKVPPSSAASPVSGALWGGGQRITARVLENFTRLLSSLLAAGVPLSRA
                     LVILHKETSSPAARAKWKEVYDLVVDGMSLADAMAKSPDTFPRVYVAMVEAGETGGFL
                     DVVLGQIADFQSREKELRAKVMAALLYPTILLVLAMAVLTFLLVFFIPRFQLIFTGFG
                     AQLPLLTQLIVSASDLLRGYGPILAVGVVVGVYWMRSWLVSEQGRRAWEGWILRMPVI
                     GPLVARFAMARFCRMLGTLLGAGVPLINGLNVARRSIGNQVLVDAVAASIERVKEGKQ
                     LGPSLSDCRHLFPGSVLEMISVAEESAKLDQELVRIADVTEADLDRQLKTAVAMAEPL
                     MLFGIAALIGTIFIGMVIPIFTLQDYIK"
     gene            4250..4879
                     /gene="gspG"
                     /locus_tag="PKK20_06020"
     CDS             4250..4879
                     /gene="gspG"
                     /locus_tag="PKK20_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNU99475.1"
                     /translation="MILPTRNASRRRPLRAHAPSPAGGVGDLCSPPRHFTVFGHARTG
                     AQTSRTGAHEAGSGGHGGAGTARALGQRAFTLIELLLVLVILGILAAIVVPKFSGRTE
                     QARETAARSQLATFGTALDAFEVDNGYYPKGRDGLQDLVVQPRDAKNWRGPYLKSDVP
                     LDPWGNAYIYECPGRYNPSGYDLMSMGPDGREGGDDDIGNWLRTDQRRR"
     gene            4899..5510
                     /locus_tag="PKK20_06025"
     CDS             4899..5510
                     /locus_tag="PKK20_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU99476.1"
                     /translation="MLTHGQLQRRTPTAIGSQASGRAFTLLELILVMALLLIVLAVAA
                     PSLAQFFRGRFLDGEARRLLALTRYGQSRAVSEGVPVLLWLNAESNQYGLRIDPRFRI
                     QDTNALCFDWSEQIEIEAGVAEFATNVMPWQASTLLSDRMVKIFFLPDGSISDISPSR
                     LRLREQDEQGRPRRQEKGEVWIVQSLNRLRYEIPEEQPLAFRR"
     gene            5470..5931
                     /locus_tag="PKK20_06030"
     CDS             5470..5931
                     /locus_tag="PKK20_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99477.1"
                     /translation="MRFPKNNRSLSGARRRCAGFTLAEVLAALVFMAIVIPVAVEGLR
                     LASQVGQVTHRKAIALRLAENILNELIVSGQWQKTPQQGTIEEGPLEFFWRVRAETWI
                     DGVLQLVSVQVLFTVQGQEYDVQLSTLADNSIPVETATSTSSSTSTSGQTR"
     gene            5928..6668
                     /locus_tag="PKK20_06035"
     CDS             5928..6668
                     /locus_tag="PKK20_06035"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNU99478.1"
                     /translation="MRIRLPTSNSRGGAFTLIEVLIATAVFAVVLVSMHGVFYGALRL
                     RNKTTAEVEKVMPLQHALAIIKRDLEGLAAPGGTLTGALRTSPTNSVAPRGRIMSGPP
                     LCTATGQLYDTLPWAELQKVTYYLAEPTNRTSGFGLDLHRAVTRNLLPLLIEDEPEDQ
                     WLMGGVERIEFWFHDGSAWRTTWDSTSETTPLPRGVRMDLELTPQPDQQYDQPVISVV
                     VPLLVQASTNTSSQANQSASGTGGSGGS"
     gene            6668..8122
                     /locus_tag="PKK20_06040"
     CDS             6668..8122
                     /locus_tag="PKK20_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNU99479.1"
                     /translation="MSPMRAPSNRFGGSPLIVLELPRRHERETFNVQLSTLDSHLPTR
                     PSAPRRGSVLIIVLWIAFGLVAVALYFAQSMLFELRAVDQRVAGAQAEQAIAGATYYV
                     SNVLAQVETPGTWPEVDLRTWEAVPIGEATVWILGRGQPIVEDEPVFDLVDEASKLNL
                     NTATREMLEKLPRMTPELAGAIIDWRDTDDDVTTGGAETEVYQRFRPAYRCKNGPFES
                     VEELRLVAGMTIEILYGEDANLNGILDPNENDGDASPPSDNRDGRLDPGLIEYLTVVS
                     REPGTRSNGSARINVTSSSQEELRTLLEEQLGEERAGTILDQVGTNNSSLGSLLEFYV
                     KSGMTPEEFCQIEGDLTVSTNSSQAGLINVNTASEAVLACVPGIGPELASTLVSQRRP
                     DATTLPTVAWVADILEQTNLVQAGPYLTSRTFQYTVDVAAVGRYGRGYRRVRWLCDTA
                     DGTPMFIRRLDVTHLGWALGATDYDSALFANRMR"
     gene            8119..>8995
                     /locus_tag="PKK20_06045"
     CDS             8119..>8995
                     /locus_tag="PKK20_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99480.1"
                     /translation="MKTWLKHVPFQRGPGGTLLGLALEAGRLEGVLVRRTNGFGEVKA
                     AFGATLSLDLLTDAPELVGREIRKHLDAAGIRERRCAVGLPLGWALTIQVPLPDLPPA
                     DIDGFLQIEAERGFPYAPEALLMAQSRFRTPGGAACATLVAIPRDHVTRLEAVLQAAQ
                     LRPVSFSLGIASLQRANAEASQGVMALAPSDHSIGLQVSCGGGVAVLRTLEGVFEIEG
                     GTPQLQADQVARETRVTLGQLPSDLREAVRRLRVFGRDNSAEALVEHLRPRFEPMGIV
                     VEQVRDYSASDTGLQL"
BASE COUNT         1579 a         2946 c         2945 g         1525 t
ORIGIN      
        1 gtggtcaact tcccgtgagc agacccggta aactccccgt gagcgccaac gccccatgca
       61 ccgaaccttg gaggggggcc gcctgcctgc cggcaggcag ggtctcgtcc ccccgccgaa
      121 gagtgttttg gaggagtttg gggcgacgag gccgtcgcca cttcgcgccg gttcatggtt
      181 ccggtgcgca ttagacagtt gaatttcaac gctgcccatg aatccgcccg ctcctcccgg
      241 cgcggcgggt gcgcacaggt tgcacagagg gagggcgagg agatgcgcaa actttgcgcg
      301 aaaggggagg gcaatcaatg caagtccttg gtgattaggt gttggcgcgg cacttgctat
      361 aggcgatgtc tcacggggct ggggcgcgct ggcgctcggc aaaccgagaa ccaaaaccac
      421 tacacaagtt ggagatagtt atgaagagaa tgctcaagag agttgtcctg accggggctg
      481 tcacactgct gtgtctgaat gtcggccaag cgctggccca gcaaggtgga ggcggcatgg
      541 ggggcggacg cgggaatttc gatccggaac agatgcggca gcggatgatg gagcggtaca
      601 aggaagcgtt cggtgtgacg gacaacagcg aatggaagat catcgaggaa cggatcaaca
      661 aggtgaatga ggcccggcgt gaaatcggct ttggcggcat ggggatgatg ggcatggggc
      721 ggcgtccggg ccagggcgga gaccaggcgc ggcccgatca ggatcgtcag cggcggatgg
      781 gtccccagcc ggatcccgcg gccgaggaac tccagaaggc gatcgaggac aaggcctcga
      841 acgacgtgct caaggcgaag atgaccgcgc ttcgcgactc cgtcaaagcg aaggaagcga
      901 aactgcaggc cgctcaggaa gacctgcgca aggtgctctc cgtccggcag gaggctctgg
      961 cgctgcagat gggtctggtc aggtagtctt gattgggtta cggacgggtc atgctcccgg
     1021 cccagtccag gagcgtggcc cggtttctca aggctccaca gcatggttgt ctccgaaaca
     1081 gaaacgacgg cgttgcgcga gttgctcgac cgcatggtgg cggccaagca gctctcgccc
     1141 gtcgacgccc agatcatcgc cagccagccc gccggcgagg atcgcacgct ggtgcggtcc
     1201 gaggaggatg tgctgcgctg gctcggcgag gaatacgatg tcccgttcac cccgctcgat
     1261 gaagtcgagc cggacaaaca ggtgctctcg ctctttcccg cgcgcatcct gctccgggac
     1321 ggtctgctgc cgctgcggcg tgtgaacggt tgcatcgagg tcgcgacgag ccggctcttt
     1381 gccacgcagg gactcgatac cctgcggacg atgacggggc tgcggctcaa ggcggtgctg
     1441 gcgcccagcg aggcgatcca gcgcgagatg aagaagcggc tcggcgtcgg ggccgacacc
     1501 atcgacaccc tcgacgagga gacgagcttc caggtcgtcg aggaaggcga cgaggaggac
     1561 acgaatctcg acaccgccgc cgaggacgcg tcgatcattc ggttcgtcaa ccaggtgttg
     1621 cgggatgcga tcgagctgcg cgcctcggac atccacctcg agccgttcga ggacgagctg
     1681 cgcattcggt accggatcga cggcgtgttg caggaggtgc ccgtgcccgc ccaagtcaag
     1741 cggttccagc cagcgatcgt gtcgcgcgtc aagattctca gtcacctcaa catcgccgag
     1801 aaacgccttc cccaggatgg gcgcatcaag atccgcgtcg acgaaagcga ggtggacatc
     1861 cgggtctcgg tcattccgat gctgcacggc gaggccgtcg tgttgcgtct gctgcgccag
     1921 agcgccatgc tgcgcggaat gcaggaattg ggcatggacg cccatgaagc gcgcagcttc
     1981 cggcgcgtcc tgggcctccc gcatggcatc gtcctggtca caggcccgac cggcagcggc
     2041 aagacctcga ccctctacac agcgcttcag gaaatcaacg attcggtccg caagatcatc
     2101 acgatcgagg acccgatcga gtaccagctc cggggcgtga accagattca ggtttccgag
     2161 aaggccggtc tgacattcgc ccgcgggttg cgctcgattc tgcgtcacga tcccgacgtg
     2221 atcctggtgg gcgaaatccg ggaccaggag acggcgcaga ttgcggtgca ggcctcgctg
     2281 accgggcatt tggtcttctc gaccctccac accaacgacg ccccgggcgc cctgacgcgc
     2341 ttggtcgaca tgggtgtcga gccctacctg gtcgcttcgt cgctcgaagc cgtggtggcc
     2401 cagcgcctcg tgcgcgtcct ctgcacacag tgcaagcagc ccgacacctc cgagacggcg
     2461 caggccctca aggcccagct cgggttccct cccgacacga tcgtctatcg cccggtcggg
     2521 tgcggcgtct gccgcaatac cggctaccac gggcgccggg cgatcttcga gtggatggac
     2581 accaatcagg agattcgtcg gatggttctc aacaaccggt cgagcgacga aatccgcgag
     2641 gcggcgcggc gcggaggtat gcggacgctt gcggaggacg gctggcgcct ggtgcgactc
     2701 ggagtcaccc cgcccgagga agtcctccgc gtcaccaaag aacaatcccc ggcgggcgag
     2761 atcgaagttg atgcgccggc cgctcccgcc ccggcggagg cggcggcgtg atccggtcac
     2821 gacccctcgc gcagccggga ttcccgaccc tcagcatgcg cagtcgacat atccagcttc
     2881 atgccgcggc gcgcgggttc tggaattccg agaactcgaa acagcgtccg aagtcccatc
     2941 gcgcgctata gaacatcagc tcaacgccat gccgacctat tcctatcgcg cgataacgcc
     3001 aagcggcacg atcaccgagg ggcagatcga ggcgggcggg cgccatgacg cttttcgcca
     3061 aatggaaggg cgcgcgctgc gccccatcag cctcaccgag ctggccggcg gacccaatgg
     3121 caagcccaac ggcaaggtgc cgccgtcgtc ggccgcctca cccgtgtccg gcgcgctctg
     3181 gggcgggggc cagcggatca cggcccgggt cctcgagaac ttcacacgcc tgctttcgag
     3241 cttgctggcc gcgggcgtgc cgctgagccg ggccctggtg atccttcaca aggagacctc
     3301 gtcgccagcc gctcgcgcca agtggaagga agtgtacgac ctcgtcgtgg atgggatgtc
     3361 cctggccgac gcgatggcga agtcgccgga caccttcccg cgcgtctatg tggcgatggt
     3421 cgaggcgggt gaaactggcg gttttctcga tgtggtgctc ggccagattg ccgatttcca
     3481 gtcgcgcgag aaggaactgc gggccaaggt catggcggcc ctgttgtacc caaccatcct
     3541 gctggtgctg gcgatggcgg tcctgacgtt cctcctggtc ttcttcatcc cgcgttttca
     3601 actgattttc accggcttcg gcgcgcagtt gccgcttctg acccaattga tcgtctcggc
     3661 aagcgacctc ctccgcggct acggcccgat cctcgccgtg ggtgtggtgg tgggcgtcta
     3721 ctggatgcga agctggctgg tgtccgagca ggggcgccgg gcgtgggagg gttggattct
     3781 gcgcatgccg gtgatcgggc cgctggtggc ccgattcgca atggctcggt tctgccgaat
     3841 gctcggcacg ttgctggggg cgggcgtccc gctcattaac ggcctgaatg tcgcgcgccg
     3901 ctcgatcggc aaccaggtcc tcgtggacgc cgtggcggcg tcgatcgagc gcgtgaagga
     3961 aggcaaacaa ctcgggccga gcctcagcga ttgccgtcat ttgttcccgg gctcggtgct
     4021 cgagatgatc tccgtggccg aagagagcgc caaactcgat caggaactgg ttcgcatcgc
     4081 cgacgtcacc gaggccgacc tcgatcgcca actcaagaca gccgtggcca tggccgaacc
     4141 gctgatgctt ttcggcatcg cggcgctcat cggcacgatc ttcatcggga tggtcatccc
     4201 gatcttcacc ttgcaggact acatcaaata accctcaacc cgcacctcca tgatattgcc
     4261 aactcgaaat gcttcccgca gacgtccact gcgcgcgcac gcaccttcgc ccgccggcgg
     4321 cgtcggcgat ctctgctcac ccccccggca tttcactgtc tttggccatg ctcggacagg
     4381 cgcgcaaacg tcccggaccg gagcccatga ggccggatcg ggcggacacg ggggcgccgg
     4441 gacggcccgc gcgctggggc agcgggcgtt cacgctcatc gagctgctgc tggtcctggt
     4501 gattctgggc attcttgcgg cgatcgtggt gcccaagttc tccggccgca ccgagcaggc
     4561 gcgggagacg gccgcccgct cgcagctcgc cacctttgga acggcgctcg atgccttcga
     4621 ggtggacaac ggctactacc ccaaaggccg cgacggcttg caggacctgg tcgtgcagcc
     4681 gcgcgacgcc aagaactggc gcgggccata cctgaaaagc gacgtcccgc ttgatccgtg
     4741 gggcaatgcc tacatctacg aatgtcccgg gcgatacaac cccagcggct atgacttgat
     4801 gtcgatggga cccgatggcc gcgagggcgg cgacgatgac atcggcaact ggctccggac
     4861 cgaccagcgg cggcgttaga caaccggcga ttttcacgat gctcacgcat ggtcaactcc
     4921 aacgccggac cccgactgcg atcggatcgc aggcctccgg gcgcgccttc acgttgctcg
     4981 agttgattct cgtgatggcc ctgttgttga tcgtgctcgc ggtggcggcg ccgtccctgg
     5041 cccagttctt ccgcggacgc ttcctcgatg gcgaggcgcg acgcctgctg gccctgacgc
     5101 gctacggaca aagccgggcg gtgtccgaag gcgtgcccgt tctgttgtgg ctcaacgccg
     5161 aatcgaacca gtacggactg cggatcgatc cgcggttccg catccaagac accaacgcgc
     5221 tctgtttcga ctggagcgag cagatcgaga tcgaggccgg tgtcgccgaa ttcgccacca
     5281 atgtcatgcc gtggcaggcc tccaccctgc tgtcggatcg gatggtcaaa atcttcttcc
     5341 tccccgacgg ctcgatcagc gacatcagcc cttcgcgcct gcgcctgcgt gagcaggacg
     5401 agcagggccg gccacgccgg caggagaaag gcgaggtttg gattgtgcaa agcctcaatc
     5461 ggctgcgcta tgagattccc gaagaacaac cgctcgcttt ccggcgctag gcgacgctgc
     5521 gccggcttca cgctggcgga agttctcgcc gcgctcgtgt tcatggccat cgtgatcccg
     5581 gtggcggtcg aggggctgcg tctggccagc caggtcgggc aggtcaccca ccgcaaggcg
     5641 atagccctgc gcctggccga aaacatcctc aacgagctga ttgtcagcgg acaatggcag
     5701 aagactcccc agcagggcac gatcgaggag ggcccgctcg agttcttctg gcgggtccgg
     5761 gccgagacat ggatcgacgg ggttctgcag ctcgtctccg tgcaggttct cttcacggtg
     5821 caaggccagg aatatgacgt ccagttgagc accctcgccg acaactcgat accggtcgag
     5881 accgccacct cgacctcgtc atcgacctcc acatccggcc agacgcgatg agaatccgcc
     5941 ttccaacctc caacagccga gggggcgctt tcaccctgat cgaggtcctc atcgcgacgg
     6001 cggtcttcgc cgttgtgttg gtgtcgatgc acggcgtgtt ctatggcgcc ctgcgcctgc
     6061 gaaacaaaac gaccgccgag gtcgagaagg tcatgccgct ccagcatgcc ctcgccatca
     6121 tcaaacgcga cctcgagggg ttggccgcac ccggcggtac cctgaccggc gccctccgga
     6181 cttcgccgac caattccgtt gccccccggg ggcgcatcat gagcggcccc cctctctgca
     6241 ccgccacggg ccagctctac gacacgctgc cgtgggccga gcttcagaaa gtcacctact
     6301 atctggccga gccgaccaac cgcacttccg ggttcggtct cgatctgcac cgcgccgtga
     6361 cccgcaacct gcttcccctc ctgatcgagg acgagcccga ggaccaatgg ctgatgggcg
     6421 gagtcgagcg catcgagttc tggttccacg acggcagcgc atggcgcacg acgtgggact
     6481 cgacgtccga gaccacgccg ttgccgcggg gcgtcaggat ggacttggaa ctgacacccc
     6541 agccggacca gcaatacgat cagcctgtga tctcggtggt cgtgccgctg ctggttcagg
     6601 cctcgaccaa cacgagcagc caggccaacc aatccgccag cgggaccggc ggctcgggag
     6661 gatcataatg agcccgatgc gcgccccatc caatcgtttc ggcggcagcc ccctgatcgt
     6721 cctcgaactg cccagaaggc atgaacgaga gacattcaac gtccaacttt caactctcga
     6781 ctctcatctc ccaactcgtc cgtccgcccc gcgccgcgga tcggtgttga tcatcgtcct
     6841 ttggattgcg tttggcctgg tggccgtcgc cctctatttt gcgcagtcga tgctcttcga
     6901 gttgcgggcg gtggatcaac gggttgcggg cgcgcaggcg gagcaggcga tcgctggcgc
     6961 cacttactac gtcagcaacg tgctggccca ggtcgagacg cctggaacct ggccggaggt
     7021 cgacctcagg acctgggagg ccgtgcccat cggcgaggcg accgtgtgga ttctcgggcg
     7081 cggccagccg atcgtcgagg acgagccggt gttcgacttg gtggacgagg cctcgaaact
     7141 caatctgaac acggccaccc gggagatgct tgaaaagttg ccccgcatga cacccgagtt
     7201 ggccggggcg atcatcgact ggcgcgacac cgacgacgac gtgacgacgg gcggcgctga
     7261 aacggaagtt taccagcgct tccgtccggc ctaccggtgc aagaacggcc cgttcgagtc
     7321 cgtcgaggaa ctgcgcctgg tcgcgggcat gacgatcgag attctctacg gcgaggacgc
     7381 caacctcaac ggcatcctcg atcccaacga gaacgacggc gacgcctccc caccgagcga
     7441 caaccgcgac ggacgcctcg atcccggcct gatcgaatac ctgaccgtgg tgagccgcga
     7501 gccggggacg cgcagcaacg gctcagcgcg gatcaacgtc acgagttcga gccaggagga
     7561 gttgcggaca ttgctcgaag agcagttggg cgaagaacgc gccggaacca tcctcgacca
     7621 ggtcggcacc aacaacagca gcctgggcag cctgctcgag ttctatgtca agagcgggat
     7681 gacgcctgag gaattctgcc agatcgaagg ggacctcacg gtctcgacca acagcagcca
     7741 ggccggcctg atcaatgtca acaccgcaag cgaagcggtg ctggcgtgtg tcccggggat
     7801 cgggcccgaa ctggcctcga cgctcgtgtc gcaacgccgg cccgacgcga ccaccctgcc
     7861 cacggtggcc tgggtcgcgg acatactcga gcaaacgaat ctcgtgcagg ccggccctta
     7921 cctgacgtcg cgcacctttc agtacacggt ggatgtcgcg gccgtggggc ggtacggacg
     7981 cggctatcgg cgggtgcgct ggctgtgcga cacggcagac ggcacgccca tgttcatccg
     8041 acgcctggac gtgacccatc tgggctgggc gctgggagcg accgactacg attcggccct
     8101 gtttgcgaac cgaatgcgat gaagacctgg cttaagcatg ttcccttcca gcgcggcccg
     8161 ggcggcaccc tgttgggcct ggccctcgaa gccggccgcc tcgagggtgt gctggtccgg
     8221 cgcacgaacg gcttcggcga ggtcaaggcc gcattcggcg ccacgctctc gctcgatctg
     8281 ttgacggacg cccccgaact cgtcggacgg gagattcgca aacacctgga tgccgctgga
     8341 atccgcgagc ggcgatgcgc cgtcggcctc cctctgggtt gggccctgac catccaggtg
     8401 ccgctgcctg acttgcctcc cgccgacatc gacgggttcc tgcaaatcga ggccgagcgc
     8461 ggcttcccgt atgcgcccga agccctcttg atggcgcaat cccggtttcg caccccgggc
     8521 ggagccgcct gtgccacgct tgtggccatt ccgcgcgacc acgtgacacg gctcgaagcg
     8581 gtcctgcagg ccgcccagct tcgtcccgtg agcttctcgc tcggcatcgc gagcctgcag
     8641 cgcgcgaatg ccgaggcgtc gcagggcgtc atggcccttg ccccgagcga ccacagcatc
     8701 ggcctgcagg tctcgtgcgg cggcggggtc gccgtgttgc gcactctcga aggcgtgttc
     8761 gagatcgagg gcggcacgcc ccaattgcag gccgaccagg tggcgcgcga gacgcgggtc
     8821 accctgggcc agttgccctc cgatctccgg gaggcagtcc gccgcctgcg ggtcttcgga
     8881 cgcgacaact cggccgaagc actcgtcgag catctgcgcc cgcgcttcga gccaatgggc
     8941 atcgtcgtcg agcaggttcg ggattactcg gcatcggata cggggttgca attgc
//
LOCUS       DAOQRO010000118         8985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7197_length_8985_cov_4.172116, whole genome shotgun sequence.
ACCESSION   DAOQRO010000118 DAOQRO010000000
VERSION     DAOQRO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7197_length_8985_cov_4.172116"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="PKK20_06050"
     CDS             <1..1627
                     /locus_tag="PKK20_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99481.1"
                     /translation="LVSTSNTVVDAPNLLYNLGSSSGLLTVQNLAKAGVIRMAGNIEM
                     WSGLWTNQMATIYTNYYIDDTTNYYNPITNPVDVGIHVVILSADFLDSTQRVVVHDFV
                     AKSKQIVVNDPITVSRKLILNGEAFTLNGTLTLTNLDTTNGLPNWVRTNMPGVKFFTN
                     NGTLTVPNVGYFGSDSGGALQTFVNTGTLNTFSANVAASSYANSGTLNIDNRLTLELG
                     AGRLEGGRDVIRGSLHIKANDLKLRNHSIRADEGLFLDVTDNLSDAGVGSPSTVSVSD
                     GFHLLAKPATGDLLGTTFQTTASKFASVLHTWAGKDLGPTAAGFKDNAAIGRLVLDVY
                     SGGELRFSGPVDWLGNPRPGKYALYVDYLQLQPAVEADPESVMYVYPGLTIYFGTSNV
                     PAEELDGMFDGRLRWVKSYSGPTSGTPVASRQNGFEIKTIVVNAALLDSTTIDSDGDG
                     VANAYDTWPFDGVTLRTAEVLGHPANQVRISWDAAAETVYRVEKTVGLTPANWTAHSV
                     VTNSAASVQVLTITDAIPAGSNQRSFYRVRYDP"
     gene            1966..5541
                     /locus_tag="PKK20_06055"
     CDS             1966..5541
                     /locus_tag="PKK20_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNU99482.1"
                     /translation="MAQAETLRLDSQEVHATRLIGKFKATVADAIGPASVSQYGSAIA
                     RRYRLVPGLVLLDEAGQARPAGLPAGDESAARQRLLDRLHALRDSGLFEYVEPDHIMH
                     LMLAPNDRAFVDGSLWGLWNYGQYGGMPGADIAAERAWDLTTGSTNVVVAVVDTGIRY
                     THLDLASQMWINANEIPDNGIDDDGNGFIDDIYGINALAGTGDPMDDHGHGTHCSGTI
                     GAAANDGNRHVGVAWEVRLMGCKAFSAQGTASTSDEITSIDYAVHMGAQIISASWGGY
                     GFSQALFDTIERAGDRGVLFVAAAGNEANDNDFFPAYPASFQLENVISVAAVDRSDYL
                     AGFSNYGATTVHVGAPGVEIFSCWAGFDADYETIDGTSMATPHVAGVAALILGLYPGS
                     DHSEMKDRILSGAVPITALAGRTTTGGRVNAYNSLTLTGSGVMQVSVDPPSGSVLLAG
                     TTQPIYVKVRDRFGIRDATVTGAIVGGGTLAFGNDGVAPDLVAQDPIYTANLQVPALG
                     DSISIALTVTAPGKGTLTNLLNYAIIPPPPNDHFELATKVPRGGASYLANNLYATLQP
                     NEPRHAGNASAAGSLWWRWTGDWETNILVDAAGSSIGTILAVYTGNALTNLKSVATAA
                     NLPGTRGTNLFFQAQKSISYSIAVAGLATNSLGSIRLRIMPGGQLQKQPPRVSIQDPI
                     SGLVSATNSITVTGTAEDVGPQASGIAEVLVSVNGGLAGSAVGTTNWMRPALLQVGAN
                     QIVARSVNLAGLISDEYRVTVHYRVPPPPNDNFDAATLVTGIEGRDTVDTRLATQQPG
                     EPMHAGKPGGHSVWWKWTAPADGALLLSTEGSSFDTTLGLYTGPRVNALRRVASNDDA
                     FDGSLYSKLQECVRMNHTYHWAVDGFNGLTGKVELAYLFTPGTLFTVNLQAATGGSVA
                     EHDSGAVDVLTYSSQTLTAVPDAQYEFAGWEGDVVSTANPVTFQVNSNLTVRPTFRAI
                     VYADDFETGTLSKLKWVPGGAKPWTVTTASVAAGKYAARSGVIQDGQTSTLSLTDRFF
                     AGSVSFDLRISSEANWDYAEFLIDGVQQMKWSGDVEWTRYSFPITTGTHTLEWRYVKD
                     NAVSSGLDAVFVDNFNAIIVPPVNSSTPARLAVRLMPQGEYQLQLTGQANQVYTLQRA
                     FELLPGRGTAWQPFATNVAVNGQVRIMLDAQTLGQSNTYFRAFAQ"
     gene            5690..6328
                     /gene="coaE"
                     /locus_tag="PKK20_06060"
     CDS             5690..6328
                     /gene="coaE"
                     /locus_tag="PKK20_06060"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNU99483.1"
                     /translation="MKVLGLTGGIGMGKSTVARMLCDHGCTVIDTDLLARQLVEPGST
                     ALADIIEVFGPGLCDGAGRLRRAEMARLVFSDVAARRRLEGILHPRIRQLWQQQVAES
                     RMAGREAAVVVIPLLFETGAEGEFDAVVCVACTPASQRQRCLARGWTPEQLEARLDAQ
                     WPIAQKIARSNYVVWSEGCLDTTIEQVRRILRQLNLHSLSASVRIETEKLVS"
     gene            6325..>8985
                     /locus_tag="PKK20_06065"
     CDS             6325..>8985
                     /locus_tag="PKK20_06065"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNU99484.1"
                     /translation="MKKTIWILGGCALVLALISWPRTSSRSSLAARGPEIARSAVQST
                     ATASTGLLAVQAIESGTLSPAPGAPGAPRVETAPVQPDARPETVFASFDTWANAFLTH
                     PSTATSQIEEGRRLAIARREALAREIKVNPRRALQRAVSWSWRQRLPSEIAAYLEEVV
                     VGRGRMQVYFASPLPGADYREFPGGKMRYVTMAGSGRTYSTYVYGRRLLQMSQDDIAI
                     HGIAVGNLLALHEDPARLLDADEGAARLAIDGIQTASTCSICGAALNEERRVLDYGGQ
                     RLVVCGLSHAVELNGILAAAEIEARRVALASGGGAPSGGNGAWTNPPPRNPVGSWGSK
                     RVLFMRLVFRDDTEVPISESEAAQVMHEVNDFYVEASYNKASLVTTVTPVLTLPYPKL
                     ACSAEGPGLILGAAIEEARKAGFVAGNYDFIVMRHPKVPGFTWGGLGGGGVAWLQGNG
                     VGLIIHELGHVFGLGHANFYDTRRAALPNNPNNNPFDIDSLVGHDSVFGVGDDVEYGD
                     VFDVMGGGGGEGPAATTNQQVSAFAGHFNAIGKNLLGWLPDPYIADVTEGGTNRIYVH
                     DTPRLVEGRQYALKVAKDAQRTYWVSARGKVQTPYTTNGVELHFSAWQQALGYSQLLD
                     TTPGSKPGRQDAPIAVGRTYADEESKVYITPTAKGGTGTNTWYDIVVHKGPQPDNVPP
                     AMELTVSTNFVRPGGSVTFTVSATDDNGDALAYYWDMSDGAVAPNSPSLTRLFANAGQ
                     YVVRVEVSDMKGGLVSKHTVITVGSPGTFRISGQVVSTDGLPLQRVCVSNGSLTEGSY
                     DYAEDYQWTYTDADGGFTLVNLSNRTYSIAAHIEGYITRPLNFETSITIADKDAEGVL
                     FMATRRPVVTVKRVSDAVVASATPGLFELTR"
BASE COUNT         1704 a         2943 c         2719 g         1619 t
ORIGIN      
        1 cctcgtcagc acctcgaaca ccgtagtcga cgcgcccaac ctcctctaca acctcggctc
       61 gagcagcggc ctcctcaccg tccagaacct cgccaaagcc ggtgtgatcc ggatggccgg
      121 caacatcgag atgtggagcg gcctttggac aaaccaaatg gcgactatct acacgaacta
      181 ctacatcgac gacacgacta actattacaa ccccatcacc aaccccgtcg atgtcggcat
      241 ccacgtcgtg atcctcagcg ccgatttcct cgactcgacc cagagggtcg tggtgcacga
      301 cttcgtcgcc aagagcaaac agatcgtcgt caacgacccg atcacggtct cccgcaaact
      361 gatcctgaac ggcgaggcct tcacgctcaa cggcaccctc accctcacca acctcgacac
      421 caccaatggc ctcccgaatt gggtccgcac caacatgccc ggcgtcaagt tcttcacgaa
      481 caatggcacc ctgaccgtgc cgaatgtggg gtacttcggc tcggacagcg gcggtgcctt
      541 gcagactttc gtaaacacgg ggactctcaa caccttcagc gccaacgtgg ccgcttcctc
      601 ctatgcgaac agcggcactc tcaacatcga caaccgcctc actctcgagc tcggggccgg
      661 caggctcgaa ggcgggcgcg acgtgatccg cggctcgctg catatcaagg cgaatgacct
      721 caagctcagg aatcactcga tccgggcgga tgagggactg ttcctcgatg tcacggacaa
      781 cttgagcgac gctggcgtgg gctcgccgag tacggtgagc gtctcggatg gtttccacct
      841 gctcgccaag cccgccacag gcgatctgct gggaacgaca ttccaaacga cggcctccaa
      901 gttcgccagc gtgctgcaca cctgggccgg caaggacctc ggccccactg ccgccggatt
      961 caaggataat gccgccatcg ggcggctggt gctcgatgtc tactcgggcg gagaactgcg
     1021 cttcagcggc ccggtcgact ggctcggcaa cccccggccc ggcaagtacg cactctatgt
     1081 cgattacctc cagttgcagc ccgctgtcga ggccgatccc gagtccgtga tgtatgtcta
     1141 tcccggcctg accatctact tcgggacctc caatgtccct gccgaggaac tggacggcat
     1201 gtttgatggc cgcctccgct gggtgaagtc atactccggc ccgacaagcg gcacgccggt
     1261 cgcctcccga cagaacggat tcgagatcaa gacgatcgtg gtgaatgccg cactcctgga
     1321 cagcaccacg atcgattccg atggcgatgg tgtcgccaac gcatacgaca catggccttt
     1381 cgacggtgtc accttgcgaa ccgccgaggt tcttggccat ccggcaaatc aagtgcggat
     1441 ttcatgggat gccgcagccg aaaccgtcta ccgcgtcgag aagacggtgg gtctcacgcc
     1501 ggccaactgg acggcgcatt ccgttgtcac caactccgcg gcatcagtgc aggttttgac
     1561 aattacagat gccatcccgg ctggaagcaa ccagagaagc ttctacaggg tccgttacga
     1621 cccgtgaacc acgggcgccg atcggaagcc gttcggcgat ccgaatggcc cagaatccgg
     1681 aatggcaggt tgatgcggat cggcgggcgg ccgcgtgtgg tcgccgccta gttttactaa
     1741 tgactcatga agaggggtac tctgatggca cagtgtcaag gatgggctgc gatgcgcatc
     1801 gcggcgttcg aagcagcctt gatccaaagg cgattcgcca gtcgcgggaa tgaccttccc
     1861 gcgacagctt cggcgcatgc gatgccccgc gagcgccgcc cggcatcgct cgcatcgctc
     1921 gcgttgactg tcggctctct cgtgtgcgcg attgccttct tgggtatggc ccaggccgaa
     1981 accctcaggc tcgattctca agaggtccat gccacccgcc tgatcggtaa attcaaggcg
     2041 actgtcgccg atgccatcgg cccggcatcg gtcagccagt acgggtccgc catcgcgcgg
     2101 cgttatcggt tggttcccgg cctggtgctc ctcgacgaag cgggccaggc gcgtccggcg
     2161 gggctccccg ccggcgatga gtcggccgca cgccaacgcc tcctcgaccg gttgcatgca
     2221 ctgagggact cgggtctctt cgaatacgtc gagccggatc atatcatgca tctgatgctc
     2281 gcgcccaatg acagggcgtt cgtggatggc tcgctctggg gcctctggaa ttacgggcag
     2341 tatggtggca tgcccggcgc ggatatcgcc gccgagaggg cctgggacct gaccaccggc
     2401 tcgaccaacg tcgtcgtggc cgtcgtcgac acgggcatcc gctacaccca cctcgacctg
     2461 gccagccaga tgtggatcaa cgcaaacgag attcccgaca acggcatcga tgacgacggc
     2521 aatggcttca tcgacgatat ctacgggatc aatgccctgg ccgggacggg cgatccaatg
     2581 gacgatcatg gccacggcac ccattgcagc gggaccatcg gcgccgcggc caatgacggc
     2641 aaccggcatg tcggagtcgc ctgggaagtc cggttgatgg ggtgcaaggc attctcggcc
     2701 cagggaacgg cttcgaccag cgatgaaatc acctcgatcg attatgccgt ccacatggga
     2761 gcccagatca tcagcgccag ttggggcgga tacggtttct cgcaagccct cttcgatacc
     2821 atcgagcggg cgggggaccg tggcgtgctg ttcgtcgccg ccgccggcaa cgaggccaac
     2881 gacaacgatt ttttccctgc gtatccggcg agcttccaac tcgagaacgt gatctcggtc
     2941 gccgctgtcg atcgcagcga ttatctcgcc ggcttctcga actacggagc caccacggtc
     3001 catgtgggcg cgccgggcgt cgaaatcttc tcatgttggg cgggatttga cgcggactac
     3061 gagacaatcg acggaacgag catggccacg ccccatgtcg ccggcgtcgc cgccctcatc
     3121 ctcggcctct acccgggctc ggatcatagc gaaatgaagg accgcattct ctccggggct
     3181 gtgccgatta ccgccctggc aggccggacc acgactggcg gccgggtcaa tgcctacaat
     3241 tcactgacct tgaccgggag cggcgtgatg caggtcagcg tggacccgcc ctcgggatcg
     3301 gtcctgctcg ccggcaccac ccaacccatc tacgtcaagg tgcgcgatcg cttcggcatc
     3361 cgcgacgcta cggtcaccgg cgccatcgtc ggcggcggca cgctggcatt cgggaacgat
     3421 ggcgtggccc ccgaccttgt ggctcaagac cccatctata cggcgaatct tcaagtgcct
     3481 gcgctgggcg attcgatttc gattgccttg actgtcaccg ctcccggcaa agggaccctc
     3541 acgaacctgc tcaactacgc aattatcccg ccgccgccaa acgaccattt cgaattggcg
     3601 accaaagtgc cgcgtggcgg cgcgtcgtac ctggcgaaca atctttacgc gacgcttcaa
     3661 cccaatgaac cgcggcacgc cggcaacgcc tctgcggccg gctccttgtg gtggcgttgg
     3721 accggcgact gggagaccaa catcctcgtc gatgcggcgg gaagcagcat cggcactatc
     3781 ctggccgtct acactggcaa cgctctcacc aacctgaagt ccgtagccac ggcggccaac
     3841 ctgccgggca cacggggcac gaacctgttc ttccaggccc aaaagagcat atcctactcg
     3901 attgccgtgg ccggtctggc gacaaacagc ctcggatcca tccgcttgcg gatcatgccg
     3961 ggcggccaat tgcagaagca gcctccgcgc gtctcgatcc aggacccgat cagcggtttg
     4021 gtgtccgcaa cgaatagcat taccgtcacc ggcacggccg aggatgtcgg cccgcaagcc
     4081 tccggcatcg ccgaagttct ggtgagtgtc aacgggggac tggcgggttc cgcggtcggc
     4141 acgaccaatt ggatgagacc cgccttgctc caggtgggtg cgaaccagat cgtcgcacgc
     4201 tccgtcaacc tcgccggact catctccgac gaataccgcg tcacggtgca ttaccgggtt
     4261 ccgccgccgc caaacgacaa cttcgacgcg gcaacgcttg tcaccgggat cgaggggagg
     4321 gatacggtcg acaccaggct cgcgacccag cagcctggcg aaccgatgca cgccggcaag
     4381 cccggcggcc actccgtgtg gtggaaatgg actgcgcctg ctgatggagc gctgctgctt
     4441 tcgaccgagg gaagctcatt cgacaccacc ctgggcctct acacaggtcc tcgagtcaac
     4501 gccctccgca gggtggcgag caacgacgat gccttcgatg gctcgctcta cagcaaactg
     4561 caggaatgtg tccggatgaa ccatacctat cactgggcgg tcgatggctt caacgggctg
     4621 actggcaagg tcgagttggc ctacctcttc acccccggca cgctctttac cgtcaatctc
     4681 caagccgcca ccggcggcag tgtcgccgag cacgactccg gcgcggtgga tgttctgact
     4741 tactcgagtc agacgctgac tgccgtgccc gatgcccaat acgaattcgc cggatgggag
     4801 ggcgacgtcg tctcgaccgc caatcccgtg acgttccagg tgaactcgaa tttgaccgtg
     4861 cgcccgacat tccgcgcaat cgtctacgcc gacgacttcg agacgggcac cctgtcgaag
     4921 ctgaagtggg tgcccggcgg ggccaaaccc tggaccgtga cgacggcgag cgtcgccgcg
     4981 ggcaagtacg ccgcgcgttc cggtgtcatt caagacgggc aaacctcgac gctgagcctc
     5041 accgaccgat tcttcgcggg ctctgtctcg ttcgatctgc gcatcagttc ggaggccaac
     5101 tgggactatg ccgagttcct gatagatggc gtccagcaga tgaagtggtc cggagatgtc
     5161 gagtggaccc gctacagttt cccgatcacg acgggaacgc acaccctcga atggcgttac
     5221 gtcaaagaca acgcggtgtc ttccgggctc gacgcggtgt ttgtggacaa cttcaatgcc
     5281 atcatcgtgc cgcccgtgaa ttcatccact ccggcgagac tcgccgtgcg attgatgccg
     5341 cagggagaat accagttgca gttgacgggc caggccaatc aggtctacac gctgcaaaga
     5401 gcgttcgagc ttctgccagg ccgtgggacc gcctggcaac cctttgcgac aaacgtggcg
     5461 gtgaacggtc aagtccgaat catgctcgat gcgcagacgc tgggtcagtc gaacacctac
     5521 ttccgcgcgt tcgcccagta gttgttccca acgccgtgtc acatcgggca gcagactgtt
     5581 cgaatcccac atctgagcgg atgcccgatc gccgcgtccg gaacgcgcgt atcatcccag
     5641 cctcgaactt cgtcctgaag tccaaggcgc atcctgcgcc attcacggca tgaaggttct
     5701 tggattgacc ggcggaatcg gcatgggcaa gtcgacggtg gcccggatgc tctgcgatca
     5761 tggctgcaca gtgatcgaca cggatttgct ggcgcgccaa ctcgtcgagc cgggctcgac
     5821 ggcgttggcc gatatcatcg aggttttcgg acccggcctg tgtgacggcg ccggccgatt
     5881 gcgccgcgcg gaaatggcgc gcctggtctt ttccgatgtg gccgcacggc gtcggttgga
     5941 aggaattctc catccccgca tccgccagct ctggcagcag caggtcgccg agtcgagaat
     6001 ggccggacgc gaggctgccg tggtcgtcat cccgctcctg ttcgagacag gggcagaggg
     6061 cgagttcgat gcggtggttt gtgtcgcctg cacaccggct tcccagcgcc agcgatgcct
     6121 cgcccgcggt tggacgcccg agcaactcga ggcgcgcctc gacgcccagt ggccgatcgc
     6181 ccagaagatc gcccgctcga actatgtggt ctggtccgaa ggatgtctgg acaccacgat
     6241 cgagcaggtg cgccgcatcc tgagacaatt gaatttacac tctctgtcgg catccgttag
     6301 aattgaaacg gaaaaactgg tctcatgaaa aaaacgatct ggattcttgg cggttgcgcg
     6361 ttggttctcg ccctgatttc ttggcctcgg accagttcga ggtccagcct cgccgcccga
     6421 ggaccggaga tagcccgttc cgctgttcag tcgactgcaa cggcctcaac cggcctgctg
     6481 gcggtgcaag cgatcgagag tggaacgttg agccccgcgc cgggcgcgcc gggtgccccg
     6541 cgcgtcgaaa ccgccccggt tcagccagac gcccggcccg agaccgtctt cgcctcgttc
     6601 gatacctggg cgaacgcctt tctgacgcat ccatccacgg caacaagcca gatcgaggaa
     6661 ggccgccgat tggccatcgc ccgccgtgaa gctctggcgc gcgagatcaa agtgaatcca
     6721 aggcgcgccc tccagcgcgc ggtgtcgtgg agttggcggc aacgcctgcc gtccgagatc
     6781 gccgcgtacc tcgaagaagt ggtcgtcggg cgcggcagaa tgcaggtcta tttcgcctct
     6841 cccctgcccg gggcggatta ccgagagttt cccggaggca agatgcggta tgtgacgatg
     6901 gccggctcgg gccgcaccta tagcacgtac gtttacgggc ggcgtctgct gcagatgagc
     6961 caggatgaca ttgccatcca cggcatcgcc gtgggcaacc tgcttgcttt gcacgaggac
     7021 cccgcacggc tcctcgatgc cgacgaggga gccgctcgcc tggcgattga cgggatccaa
     7081 accgcttcga catgctcgat ctgcggcgcg gccctgaacg aagagaggcg cgtgttggac
     7141 tacggggggc agcgccttgt cgtctgcggg ctcagccatg ctgttgaatt gaatggcata
     7201 ctcgccgccg ctgagatcga ggcgcgccgc gtggcgttgg cctccggcgg aggggcgccc
     7261 tcgggtggca atggtgcttg gaccaatccg cccccccgca atccggtcgg gtcgtggggc
     7321 agcaaacggg ttctcttcat gcgcctggta tttcgcgacg acaccgaggt gccgatttcc
     7381 gagtctgagg cggcccaggt gatgcatgaa gtcaatgact tctatgtcga ggcctcatac
     7441 aataaggcca gtctggtcac gaccgtcact ccagtcctca cgctgccgta tccaaaactc
     7501 gcttgctccg ccgaaggccc cggcctgatc ctcggcgctg cgatcgagga ggcccgcaag
     7561 gcgggattcg tggccggtaa ttacgacttc atcgtcatgc gccatcccaa ggttcccgga
     7621 ttcacgtggg gcgggctggg agggggagga gtcgcttggc tccagggcaa cggggttggc
     7681 ttgatcatcc acgaactcgg gcacgtgttc ggcttgggcc acgccaattt ctacgacacg
     7741 cggcgggcgg cgctgccgaa taacccgaac aacaacccgt tcgacatcga tagcctggtc
     7801 ggccacgaca gcgtcttcgg cgtaggcgac gatgtcgagt atggcgacgt tttcgatgtc
     7861 atgggcggcg ggggcggcga gggccccgcg gctaccacca accagcaggt atctgctttc
     7921 gcgggccact tcaatgccat cggcaagaat ctcctgggct ggcttcccga cccctacatt
     7981 gccgacgtga cggagggggg caccaaccgg atctacgtcc acgatacgcc caggttggtc
     8041 gagggccgac agtatgccct caaggtggcc aaggacgcgc agcgcaccta ttgggtcagc
     8101 gcgcgcggaa aggtccagac accgtacaca accaacggcg tcgaactgca cttcagcgcg
     8161 tggcagcagg ccctgggata cagccagctt ctcgacacaa ccccgggctc gaagccgggg
     8221 cgccaggacg ctccaatcgc agtcggacgg acttacgcgg atgaggaatc caaggtctac
     8281 atcacgccca cagccaaagg cggcacgggc accaacacct ggtacgacat tgtcgtccac
     8341 aaaggacccc agcccgacaa cgtccctccc gcgatggaat tgactgtgtc cacgaacttc
     8401 gtccgccccg gggggagcgt gacgttcacc gtcagtgcca ccgatgataa cggcgatgcg
     8461 ctcgcctatt actgggacat gagtgacggt gccgtggcgc ccaacagtcc gagtttgacc
     8521 cgcctcttcg ccaatgccgg ccagtacgtc gttcgcgtcg aagtcagcga catgaaaggc
     8581 ggccttgtca gcaaacacac ggtcatcacc gtcggctcgc ccggcacctt ccgcatcagc
     8641 ggccaggtcg tcagcaccga cggcctgcct ctgcaacggg tctgcgtttc gaatgggtcc
     8701 ctgaccgaag gttcatacga ctacgccgaa gactatcagt ggacatacac tgatgcggat
     8761 gggggtttca cgcttgtgaa tctgagcaat cgcacctact cgatcgccgc ccacatcgag
     8821 ggctacatca cccgaccgct caatttcgaa acctcgatca cgattgcgga caaggacgcc
     8881 gagggcgtct tgttcatggc gacgcgcaga cccgtcgtga cggtcaagcg agtcagcgac
     8941 gccgtggtcg cctccgcaac gcccggtctc ttcgagctga cgcga
//
LOCUS       DAOQRO010000119         8967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7224_length_8967_cov_4.124663, whole genome shotgun sequence.
ACCESSION   DAOQRO010000119 DAOQRO010000000
VERSION     DAOQRO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7224_length_8967_cov_4.124663"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..1650
                     /locus_tag="PKK20_06070"
     CDS             415..1650
                     /locus_tag="PKK20_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNU99485.1"
                     /translation="MLNDDVSSGGGEPPSHPSFESLKAFRSAGRGPWAQVADSDWNDW
                     RWQMRHRITTLEQLERLLPALAPEERAGTDLVAAKLSMGITPYFFNLIDLSDPLCPIR
                     RQVVPRLEETDRAPWELTDPCGEDRHSPVPGLVHRYPDRVLFLVTNRCASYCRYCTRS
                     RLVSNASGYGFHPDFERQLDYIARTPQVRDVLLSGGDPLVFSDDKLEHLLNRLRAIPH
                     VEFLRIGTRIPIFLPQRITPDLCGMLRRFHPLFVSIHSNHPHELTIEAREALGRLADA
                     GIPLGNQSVLLRGINDSVEVMRAHVQKLLLCRVRPYYLYQCDLIAGSAHFRTSVQKGL
                     EIIEGLRGYTTGYAVPTFAIDAPGGGGKVPVSPDYVLSRNAERVVIRNYEGKIFEYPE
                     ALDCAAPCRAGQGAQSDWA"
     gene            complement(1705..1914)
                     /locus_tag="PKK20_06075"
     CDS             complement(1705..1914)
                     /locus_tag="PKK20_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99486.1"
                     /translation="MTLTSMVGSNDFVIQWRSVSNAFYTVLPWDAIGANPFVVERNLP
                     ATPPTNSLTRPIVSGARLYRVLIEP"
     gene            complement(1975..2139)
                     /locus_tag="PKK20_06080"
     CDS             complement(1975..2139)
                     /locus_tag="PKK20_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99487.1"
                     /translation="MASLIGVEDCWEKAHSGGQRGYDLLVLMLSNKACRQPYLIDTTA
                     WTPGRHTLFI"
     gene            complement(2269..3183)
                     /gene="ftsY"
                     /locus_tag="PKK20_06085"
     CDS             complement(2269..3183)
                     /gene="ftsY"
                     /locus_tag="PKK20_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNU99488.1"
                     /translation="MGLFQKIKAGLQKTHAQLTHQIKRIVTGSPRLTGSTLEELESAL
                     LGADLGCATTTRIVAAVKKAYETQGNSGVDVFAIAQGEVATGLASNDATLRRAPGGLT
                     VVSIIGVNGTGKTTTCAKLAHLVQSQGDRAVLAACDTFRAAAIEQLKVWGERLGVEVV
                     AGAYSADPAAVAHDAISAAQARGAHYLFVDTAGRLHTKNNLMKELQKVHRVMDKKLPG
                     APHEVLLVLDATTGMNALNQAREFHKAVSLTGLIVTKLDGTSKGGMVVAIQKELELPI
                     KFIGVGEQPDDLQPFDARQFAEALFRQA"
     gene            complement(3208..4134)
                     /locus_tag="PKK20_06090"
     CDS             complement(3208..4134)
                     /locus_tag="PKK20_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNU99489.1"
                     /translation="MFADLHLHTRFSDGTYSPEELAGHAQRHGLSVLALTDHDSVEGC
                     GAMTTACARLGLEFIAGVELTAEHDGAELHLLGYFLDTQHPRLLRELSRFQGVRQDRI
                     REMVARLNHLRVPLEAEAVFALANCRSPGRPHVARALVQAGLCRTLDEAFDRFLKKHR
                     PAWVPKFKMSATQAIALIHEAGGLAVMAHPGLNHNDRLIPHMAEAGLDGLECYHSRHS
                     LAASEHYLGLARKHVLLVTGGSDCHGMNRGRPLIGSVHLPYKYVVILKERVAQRKSRS
                     AACTPNASRPDTPTHPLLPSATKPPPPSCAVA"
     gene            complement(4175..4675)
                     /gene="nusB"
                     /locus_tag="PKK20_06095"
     CDS             complement(4175..4675)
                     /gene="nusB"
                     /locus_tag="PKK20_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNU99490.1"
                     /translation="MGTRREARERAVQFLFQHDLNPPTDLDAALGRFWESQRAAALQA
                     ETTGATWGQPPALPPPSAKESVVRLFADPLIRGTLEHRDEIDAHIRRIAKNWELHRIA
                     VVDRNILRLAIFEMLHREDIPPVVSINEAIDIAKKFSTEESGKFVNGILDRIRGELMR
                     PSRIVS"
     gene            4772..6157
                     /locus_tag="PKK20_06100"
     CDS             4772..6157
                     /locus_tag="PKK20_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNU99491.1"
                     /translation="MLIWLIVFAIFAILGVTGYYKGAIRSAVSLIGLGFAIFLALPLS
                     PLLRPLVPKLGLINPVWDHILPPAVVFLVVALIFVGLGFLVHFKVAHHFKYATDDYTR
                     IRWERLNRVLGLCIGLTAGGVCTILVGLMIYIFGYPAVQLTSEESPLWQRLLATARKD
                     LQTSGMDRTMAALDLMNEKYYLSTDLVGLLYQNPVVLDRLLNYPAFLALSERDDVKDL
                     TTDTDFLNGWQTKRPILDIVNHSKVMGLIKNSEFGNEVRQADLKDLYRYLGSGKSDKY
                     DEEKILGRWQLDVASTFTLTRKKNPDMTVGEMTQMRLLITVFLPKLSFMATPDNRAFM
                     RVELTDQAKQMIEAARAAIAAAQRAAEEAGTVAPQMDARMMQRYGLRPGQRPGLPPPD
                     ETQKPKAPEQPLPGIPEVRFSGEGTWERVGTKYKLTIKNEKGQQVSGEGTADEDRLLL
                     TLDGQPLVFIR"
     gene            complement(6147..6416)
                     /locus_tag="PKK20_06105"
     CDS             complement(6147..6416)
                     /locus_tag="PKK20_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99492.1"
                     /translation="MDDQRVRVGLIAETVAREFAHGLFQTNARGRFAARALSRSLQDF
                     VEIMIEWMRTSYGLDPVCAELVFGAADSELPIWEVDLAGDRRTTG"
     gene            complement(6409..7797)
                     /locus_tag="PKK20_06110"
     CDS             complement(6409..7797)
                     /locus_tag="PKK20_06110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU99493.1"
                     /translation="MRISNLNPAHDIGASSWLIDIEGHRLLLDAGTHPKREGRDALPL
                     YERIPAGELDAIVISHCHHDHVGSLPIAARRYPGAHVLMTELSYFLVERVLHNSVNVM
                     VRQRDELGIREYPLYSHDEVDDLAPRIQGFRYEREIEWAAHHKMRAGFVSPTIEFRDA
                     GHTLGAAGVMVRGQRETLFYTGDVSFHDQTLLKAARFEDVQADVLLMETTRGNRQTPA
                     SFTREAEAERLAEAIRATLKRKGSVLIPSFALGRTQEILGLLALLMRSGHIRRQPVYV
                     SGLGRVFTEIYDLEAHRTHRQHSNLMLNQALDLVVLERGQIDQMRLTGGRMFVLTSGM
                     MSENTAAHDLALRFMGEERHAILFVGYVDPQTPGGRLKASHPGRPFLFSRTAGEAVRR
                     CEVAEFDLTAHANRQDLVDFVGQVAPRTVVLGHGEPEAQAWFETTLRKRFPKIKCLRP
                     GPGETAEPVAYG"
     gene            8357..8845
                     /locus_tag="PKK20_06115"
     CDS             8357..8845
                     /locus_tag="PKK20_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNU99494.1"
                     /translation="MSFREKIVGFQDLDAWRKRQKAEGRRVVVTNGCFDLLHLGHVVY
                     LEAARALGDCLLVGLNSDSSVRSLKGPTRPIHPEQDRAGVLAALASVDAVCIFPDLDA
                     LRFLSVARPDVYAKGGDYTIDTINQPERRLVEANGGRVVVLAGVEGRSTTSTLDRMRS
                     SS"
BASE COUNT         1604 a         2670 c         2913 g         1780 t
ORIGIN      
        1 gagcttctcc tggttcggcg tcaacatgct cggcgtgggt ctgcactcgt acgggttcat
       61 ggaccaggcg ttcgtctggc tgatggtctt cgtcgtcagc cagttgctct tcatcgggtt
      121 ggcattttcg agcgggcctt cgggtgcaag gtgtcgacgc tgaccgagaa ggccatggcg
      181 aagcagacgt agcccttgtg gatttcacgg gttgccttgg cgagcaggaa caggccgtcg
      241 acgatcagga tcagaagccg atccgcaccg gagaatacaa cgagcgcagg ccggggttcc
      301 tctctgtcgg caggcaggcc gggcccggcg ctctcaacgc ggtcacggtg agaagcgctt
      361 gcgccgccgg cgggtcgctt gacaggtccg acctccctcc tatagttcgt gtcaatgttg
      421 aatgacgatg tttcgtccgg cggcggggag ccgccatcgc acccatcctt cgaatcgctg
      481 aaggcgtttc ggtcggctgg acgggggccg tgggcgcagg ttgccgacag cgactggaac
      541 gactggcgct ggcaaatgcg gcatcggatc acgacgctcg aacagctcga acgtttgttg
      601 ccggcgctcg ccccggaaga gcgggcaggg accgatcttg tcgccgccaa gctgtcgatg
      661 gggatcacgc cctatttctt caatctgatc gatttgtccg atccgctttg cccgattcgg
      721 cgccaggtgg tgccgcggct cgaagagacg gaccgagcgc cctgggaact gacggaccca
      781 tgcggggagg accggcattc gcccgtgccg ggtttggtgc atcggtaccc ggatcgcgtg
      841 ctgttcctgg tcacgaatcg ttgcgcttcg tattgtcggt attgcacgcg ctcccggctg
      901 gtcagcaacg cgtccggcta tggttttcat cctgacttcg agcgccaact cgactacatc
      961 gcacgcacgc cccaggtgcg cgacgtgctg ctgagcgggg gcgatccgct ggtgttcagc
     1021 gacgacaaac tcgagcatct gctgaatcgc ttgcgggcca ttccgcacgt cgagtttctg
     1081 cggatcggga cgcgcattcc gattttcctg ccgcagcgca tcacgccgga tttgtgcggc
     1141 atgctccgcc gatttcatcc cttgttcgtc agcattcact cgaatcatcc gcacgagttg
     1201 accatcgagg cgcgcgaggc gttggggcgt cttgcggacg cggggattcc ccttggcaac
     1261 caatccgtcc tgctcagggg catcaacgac agcgtcgagg tcatgcgcgc ccatgtccag
     1321 aagctcctgc tctgccgggt ccgcccgtac tatctttacc aatgcgattt gattgccgga
     1381 tccgctcact tccggaccag cgtccagaag gggctcgaga tcatcgaggg tctgcgcggt
     1441 tacacaacgg ggtatgcggt gcccacgttt gcgatcgatg cgcccggcgg cggtggtaaa
     1501 gtgccggttt ctccggacta cgtgctcagt cgcaacgcgg agcgggtggt gattcggaac
     1561 tatgaaggca agatcttcga gtatccggag gcgctcgact gcgctgcgcc gtgccgggcc
     1621 ggccagggcg cgcagtcaga ctgggcttaa cctgaggagg cgctggttcc gagacccggt
     1681 ccgatcgagc gacaagctcc gcactcaagg ctcgatcaac acgcggtaga gtcgggcgcc
     1741 agagacaatg ggacgagtca ggctgttggt gggaggggtg gcggggagat tccgctcgac
     1801 gacaaagggg tttgccccga tggcgtccca cggcaggacg gtatagaacg cgttgctgac
     1861 actccgccac tggatgacga agtcgttgga ccccaccatg ctcgtcaggg tcaacggctc
     1921 gatccagaaa aacagggcgg tgggggcacc ccaattgccg gcggtgtctt gcgtctagat
     1981 gaacagcgta tggcggccgg gcgtccaggc cgtggtgtca atgaggtaag gctggcggca
     2041 agccttgttc gagagcatca gcacgagcag gtcgtagccc cgctgtccgc ccgaatgggc
     2101 cttctcccaa cagtcctcaa cgccgatcag cgaggccatc tcgggatgct gcgccgcgat
     2161 tcgggccagg gcatcgcggg tctcctcgac ggtccggtct ggaatcctgg cggattgggc
     2221 aggcgcgcgt tgcgggcgtg cgttggccag cgccgtgagc cccgagtcct acgcttgacg
     2281 gaagagggct tcagcgaatt ggcgggcgtc gaagggttga aggtcgtcgg gttgttcgcc
     2341 gacgccgatg aacttgatgg ggagttcgag ttccttctga atggcgacga ccatgcctcc
     2401 cttgctggtt ccgtcgagtt tcgtcacgat caatccggtc aacgagacgg ccttatgaaa
     2461 ctcgcgcgcc tggttgaggg cgttcatccc ggtggtcgca tcgagcacca gcaggacctc
     2521 atgaggcgct ccgggcagtt tcttgtccat gactcggtgc actttctgga gttccttcat
     2581 gaggttgttc ttggtgtgca gacgcccggc ggtgtccacg aacaggtagt gcgcgccccg
     2641 cgcctgggcg gccgagatcg cgtcatgggc caccgcagcc ggatcggcgg aataggcgcc
     2701 ggcgaccacc tcgaccccga gtcgctctcc ccacaccttc aattgctcga tggcggccgc
     2761 gcggaacgtg tcgcaggcgg ccaggacggc gcggtctccc tgcgattgca cgaggtgagc
     2821 gagcttggcg cacgtcgtgg tcttgcccgt gccgttcacg ccgatgatcg acacgacggt
     2881 taggccaccg ggtgcacggc gcagagtggc gtcgttcgag gcaaggcctg tcgccacctc
     2941 gccctgagcg atggcgaaga catctacgcc gctgttgccc tgggtctcgt atgccttctt
     3001 gacggctgcg acgatccggg tggtcgtcgc acagcctaaa tcggcgccga gcagtgcgga
     3061 ttccagttct tcgagggtcg atccggtgag gcggggggat cccgtcacaa tccgcttgat
     3121 ctggtgcgtc agttgggcgt gcgttttctg cagtcccgcc ttgatttttt gaaacaggcc
     3181 catgatgcca gggagagtga gagcggctca ggcgaccgcg caggaaggcg gcggcggctt
     3241 ggttgcgctc gggagcagag ggtgagtcgg cgtgtcaggc cgggaggcgt tcggcgtgca
     3301 ggccgcggag cgggacttgc gttgggccac tcgctccttg aggatgacca cgtacttgta
     3361 ggggaggtgg acgctcccaa tgagcggacg ccctcgattc atcccgtggc aatcggagcc
     3421 gccggtgacg agcaagacat gctttcgcgc caggcctaga taatgttcgc tcgccgcgag
     3481 ggagtgtcgg gaatggtagc actcgagacc gtcaagcccg gcctccgcca tatgggggat
     3541 taaccggtcg ttgtggttga ggcccgggtg cgccatgacc gccaatcctc ccgcctcgtg
     3601 aatcaatgca atggcctggg tggcggacat cttgaacttc ggaacccagg ctgggcgatg
     3661 ttttttgaga aaacgatcga acgcctcatc gagtgtccga cacaaaccgg cctgcaccag
     3721 ggcgcgggcc acatgcgggc ggcccggtga ccggcaattg gccagggcga acacggcctc
     3781 ggcctcgagc gggacccgga ggtggttgag ccgggcaacc atctcgcgaa tccgatcctg
     3841 gcgaacgccc tggaagcggc tcagttcgcg cagaagccgt gggtgctgcg tatcgaggaa
     3901 gtagccgagg agatgcagtt cggcgccatc gtgttcggcg gtcagttcga cgcccgcgat
     3961 gaattcgagt cccagccggg cgcaagctgt cgtcatggcg ccgcatccct cgaccgaatc
     4021 gtggtcggtg agagcgagga ccgagagacc gtgtctttgg gcgtggccgg ccaactcctc
     4081 cgggctgtaa gttccgtcgg aaaagcgggt gtggaggtgg aggtcggcaa acattgcgtg
     4141 ggttggtggt gtgattctgg agtgcccggt tcggttatga gacgatgcgg gacgggcgca
     4201 tgagttcgcc ccgaatcctg tcgaggatgc cgttgacgaa cttgccgctt tcctcagtcg
     4261 agaatttctt cgcgatatcg attgcctcgt tgatgctgac cacgggtgga atatcttctc
     4321 ggtgcaacat ctcgaagatt gccagacgca ggatgttgcg gtcaaccacg gcgattcgat
     4381 gcagttccca attcttggcg atgcggcgga tgtgcgcgtc gatctcgtcg cgatgttcca
     4441 gcgttccccg gatgagcgga tcggcaaaca ggcggacgac gctttccttg gcgctggggg
     4501 gcgggagagc gggcggttgc ccccaggtgg cccctgtggt ctcggcttgg agggcggcgg
     4561 cgcgctgcga ctcccagaaa cgaccgagcg ccgcatcgag atcggttggc ggattcaaat
     4621 cgtgctgaaa gagaaactgc acggcgcgtt cgcgcgcctc gcggcgtgtg cccatgcctt
     4681 caggatgggg aagcaccccc cctgaggaaa gcaaaaatgt cggcacattt tttgcgtgat
     4741 ttccccagtg agattgaatt aggttctgcc tatgctcatt tggcttattg tctttgcgat
     4801 tttcgcgatc ctgggcgtga ctggttacta taaaggagcc attcggtcgg cggtctcttt
     4861 gatcggactc ggcttcgcca tcttcctcgc tttgcccctt tcgcccctct tgcgtccgct
     4921 ggttcccaag ctcggtctga tcaaccccgt ctgggatcat attctgccgc ccgcggtggt
     4981 ttttctggta gtcgccctca tcttcgttgg tctcggcttt ttggtgcatt tcaaggtcgc
     5041 ccatcatttc aagtacgcca cagacgatta cacacgcatc cgttgggaac ggctcaaccg
     5101 tgttttgggt ctgtgcatcg gcctgaccgc cgggggcgtg tgcacgattc tggtggggct
     5161 gatgatctac atcttcgggt atccggccgt ccaactcacc agtgaggaaa gtcctctctg
     5221 gcaaaggctt ctggccacgg cacgcaagga tctccagacg tcgggcatgg acaggacgat
     5281 ggcggcactc gatttgatga acgaaaagta ctacctgtcg accgacctgg tcggcctgct
     5341 ctatcagaac ccggtggtcc tggaccgctt gctcaattac cctgcctttc tggctctgag
     5401 cgagcgcgat gacgtgaagg acctgaccac ggacaccgat tttctcaacg ggtggcagac
     5461 caagaggcca atcctcgaca tcgtcaatca ttcgaaagtg atggggctga tcaagaactc
     5521 ggagtttggc aacgaggtga gacaggcgga cctcaaggat ctgtatcggt acctggggag
     5581 cggcaagtcc gacaagtacg acgaggagaa aatactgggc cgatggcaac tggatgtggc
     5641 gtccacattc accctcaccc gcaagaagaa cccggacatg acggttggtg agatgaccca
     5701 aatgaggctt ttgatcacgg tctttctgcc caagctgagc ttcatggcca cgccggacaa
     5761 cagggcgttc atgcgagtcg aactcaccga tcaagccaaa cagatgatcg aagccgctcg
     5821 agctgccatt gccgccgctc agcgggcggc tgaagaggcc ggcacagtag ccccgcagat
     5881 ggacgcccgt atgatgcaga ggtatggact gaggccaggg cagcgccccg gattgccgcc
     5941 gccggacgag actcagaagc ccaaggcccc ggaacaaccg ctgccgggta tccccgaagt
     6001 tcggttcagc ggtgagggca cttgggagcg agttggcacg aagtacaaac tcaccatcaa
     6061 gaatgagaaa ggccaacagg tcagcggcga gggaacggcg gacgaggatc ggttgctcct
     6121 gaccctggat ggccaaccgc tggtctttat ccggtagttc tccgatcgcc ggccagatca
     6181 acctcccaaa tcggcaactc ggaatcggcg gcaccaaaga ccagttccgc gcataccggg
     6241 tcgagaccgt aggaggtgcg catccactcg atcatgattt cgacaaagtc ctgcagcgag
     6301 cggctcagcg cgcgggcggc aaaacggcca cgggcatttg tctgaaagag cccgtgggca
     6361 aactcgcgtg ccaccgtttc ggcgatcagc ccgacgcgca cgcgctggtc atccataagc
     6421 gacaggctcg gcggtttctc cggggccggg gcgcaggcat tttattttgg ggaaacgctt
     6481 gcgaagggtc gtttcgaacc aggcctgcgc ctcgggttcg ccatgcccaa gcacgaccgt
     6541 tctcggcgcg acctggccca caaaatcgac caaatcctgg cggttcgcgt gcgcggtcag
     6601 gtcgaactcc gcaacctcgc atcggcgcac cgcctcgccc gcggtccggc tgaagaggaa
     6661 ggggcggcct ggatgcgacg ccttgaggcg tccgccgggg gtttgaggat cgacatagcc
     6721 gacgaagagg atggcatgcc gttcctcccc catgaaacgg agcgccagat cgtgcgcggc
     6781 cgtgttctcg gacatcatcc cggacgtgag cacgaacatg cgccctccgg tcaggcgcat
     6841 ctggtcgatt tgtccgcgtt cgaggaccac gagatccaac gcctggttga gcatcaggtt
     6901 gctatgctgc cggtgagtcc ggtgcgcctc caggtcatag atctcagtga agacacgccc
     6961 caagccgctg acgtaaaccg gctggcggcg gatgtgcccc gaccgcatca gcaaagccag
     7021 cagccccagg atttcctggg tgcgtcccag ggcgaacgag gggatcaaca ccgatccttt
     7081 gcgcttgagt gtggcacgga tggcctcggc cagccgctcc gcctccgcct cgcgcgtgaa
     7141 gctcgcgggc gtctggcggt tcccacgcgt ggtttccatg agcaaaacgt cggcctgaac
     7201 gtcctcgaat cgggctgcct tcagcagggt ctggtcatga aacgaaacgt cgcccgtgta
     7261 gaaaagcgtc tcgcgttggc cgcgcaccat cacgcccgcc gcacccagag tgtggccggc
     7321 atcccgaaac tcgatcgtcg gtgagacaaa tccagcccgc attttgtggt gcgccgccca
     7381 ttcgatctcc cgctcgtacc ggaatccctg gatgcgcgga gcgaggtcgt cgacctcgtc
     7441 gtggctgtag agcgggtatt cgcgaatgcc cagttcgtcc cgctggcgca ccatgacgtt
     7501 caccgagttg tggagcacac gctcgaccag aaaatagctc aactccgtca tcaatacatg
     7561 cgcgcccggg tagcggcgag cggcaatggg caacgatccc acatgatcgt ggtgacaatg
     7621 cgagatgaca atcgcgtcca gttctcccgc gggaatgcgc tcgtagagcg gcaaggcgtc
     7681 acgcccctcg cgtttggggt gggtcccggc atcgaggagg agccgatgcc cctcgatgtc
     7741 gatcagccag ctgctggcgc cgatatcgtg agccgggttg aggttagaaa tgcgcatcgt
     7801 aagggaagcc cctctccctc atccatgcat ttgctctgcc tgcccgtccg atgcgccctc
     7861 caggtggcca gaggcgccat tgcatcgggc gattcgcagt tcttcttgtt tgaacgaaat
     7921 gcatgcctgg gcagtcgatt cccttgttgg gctgactgtc tgctacacaa agccccaacg
     7981 acgcgcggca ggccaacgca cctgccgccg tccgccagtg gagaggaggt cgtttggttg
     8041 tctggttgtt tgggttaggc gggcatctcg cgatgcccgc cttaccgttg tcgaatgctg
     8101 agattccgat ccgattctgt caagggcccc ccccattcgg cccgagcagt tcttcatgaa
     8161 tcaggttcat gacaagactt cccgttgcat caggaccgat tcatgaaggc gaaaaagccg
     8221 gagtggggga tggctcgtcc cccgccatgt caagtgaagg cgtcgccgct ccacttcatg
     8281 aattggcgct gccgttgcat tcggcggtcg atttggccgt tgtcatacac aatcgatcgc
     8341 gctcagtatg tggcccatgt cctttcgaga gaagatcgtg ggattccagg atctggacgc
     8401 ctggcgcaaa cggcagaagg cggagggccg ccgggtggtt gtgaccaacg ggtgcttcga
     8461 cctgctccat ctgggccatg tggtgtatct cgaagcggcc cgcgcgttgg gcgactgcct
     8521 cctggtcgga cttaacagcg attcgtcggt ccgctcgctc aagggtccca cccggcctat
     8581 ccatcccgag caggatcgcg ccggcgtcct ggcagccctg gcctcggtcg atgccgtttg
     8641 catctttccc gacttggacg ccctgcgttt cctgagcgtt gcccggccag acgtttatgc
     8701 caagggcggc gactacacca tcgacaccat caaccagccg gagcgccgct tggtggaagc
     8761 caacgggggg cgggttgtgg tgctcgcggg cgtcgaaggc cgatcgacaa cctcgaccct
     8821 cgatcggatg agatcgtcct cgtgagatcc gccggctgcg agggcgcacg gtcttttcat
     8881 ttggagcggt gcttgacgag tatctggaaa gggattcaca gccatcccac aggaattggc
     8941 gaaaaaaatg gttggaagct tgacgaa
//
LOCUS       DAOQRO010000120         8964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7228_length_8964_cov_5.692783, whole genome shotgun sequence.
ACCESSION   DAOQRO010000120 DAOQRO010000000
VERSION     DAOQRO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_8964_cov_5.692783"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKK20_06120"
     CDS             complement(<1..240)
                     /locus_tag="PKK20_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNU99495.1"
                     /translation="MATAILIDGDFFLRRYRRLVGQQNSNKTATDLHWMCREHLKQPD
                     GKRELYRIFFYDCPPLTKKAHNPLTGKAVDFILDPM"
     gene            complement(371..994)
                     /locus_tag="PKK20_06125"
                     /pseudo
     CDS             complement(371..994)
                     /locus_tag="PKK20_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359196.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(1131..7277)
                     /locus_tag="PKK20_06130"
     CDS             complement(1131..7277)
                     /locus_tag="PKK20_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNU99496.1"
                     /translation="MKCHALVLLVVLLMKPSLHAASRDAQWKRVDEAIEKGLPQTAVK
                     ELTPIIQGALADRAWAEAVKAIVRRIALDGEAQAGKPGENIRRLDAEISGAPAEIKPI
                     LQTILAHWYWQYFRKNRWRFMQRTATAEAPGEDFETWDLPRLFAEIDRRFTIALSAET
                     ELRTIRISAYDDLLPRGALPDVYRPTLFDFIAHEALSFYTSGEQAGALPQDAFQVAAD
                     SPALGPVNAFLNWDPTVGGGSTQEPVSPLIKAIRLYQALLRFHAGDADQTAFLDLDLA
                     RLVWAKNNAVGEGKAARFQAAMKQFAGTHGDHELASMALWHWAQALREQNDLAQAHQL
                     AVRARQIHPKSAGGKLAANLVAEIESKSASIRTERIWNRPWPAIEVRYRNVDQAHFRL
                     ISANWDDHLDRSRSRPENLRDDERAAILARPAAYTWSRPLPPTTDFLEHVVEVTVPDD
                     LKPGFYFLIASHRPDFAEKDNQLAMTDIWVSDLALVLRQDASQLDGFVLEAGSGEPIR
                     GAQVQAWYLDRQGQRVPVPALTTDQDGRFALALGEQQRNYVLKATANGQAIASADQFT
                     GWGRTPQRRPFEQTLFFTDRALYRPGQTVQYKGICLRADQDKDTYETLAGRTLTVVFR
                     DGNRKEIARQQHRANDYGSFSGSFTAPRDRLTGQMELCAIEGPPGAGWVNVEEYKRPK
                     FQVTLDAPKTGAKLGDTLEVVGHAMSYTGAAVDNAEVKYRVRREVRMPWWWGWYGWRR
                     GGFQPGVSQEIAHGTLRTGADGAFAVEFVARPDAAVPVEDDPTFIFSVHADVTDSAGE
                     TRSDERAVRLGYTALEAQLTADDWLTDEQPVELRVRATTLDGEPQQAEGILRVHALKA
                     PDRVSRPSLFKQWGGDEDGDDGAAAGAPGADLSNPNRWDMGAAVWEGGFTTDAKGESS
                     AKVKLPAGAYRAVLETQDRYGKKVTGRLPLTVVRPTDKAFPIKVPSFLQAADWTVEPG
                     SEFTALWGTGYGEGRAYVEIEARGRTLRKYWTSPGETQALIRQPIDEAMRGGVTLRVT
                     QVRENRAYLETRQIAVPWRNKNLTLEWEHFTSKLEPGKKETWSLVIKPAAPSAEAVGV
                     NNASNSKRLPELVATLYDRSLDAFLPLHWPNRFSVFRQEWTGRQAAFGNQARGLGVFR
                     QDWPVSFEAVLEGYRAFPPDLIQTGWGFPAATMLMRAGGLPTRRGRGMVAAGAIESLS
                     LPAAVPAPPMSPPGEAAAEMTMLMRYGLKAAPDDGAGAAGGAAVEGAGAAAADLNLVS
                     ARRNLNETAFFYPHLVADAQGVVRLSFTMPEALTEWRVMAFAHDRALRSGFIEAKAVT
                     AKDLMVQPNPPRFLREGDVVEFTVKVTNSGDKPQRGKVRLTFNNGFDNQPADALLGNR
                     NSERSFDLAGGASTSVAWRLTVPEGLSVLTYKAVATAGTVSDGEEGLVPVLARRIPVT
                     ESLPLPIVGPGTNRFEFTALLDAGKSRTLRHQSLTVQMVSNPAWYAVLALPYLMEFPY
                     ECNEQVFNRYYANALARFIANSNPKIRRIFDQWKATPALDSPLEKNQDLKGVMLEESP
                     WVCEAQKESEARRNVGVLFDENRLDSEQARALDKVREAQLDDGTWPWFPGGSRSDYIT
                     LYIVAGFARLRHLGVDVDVAPAVRALDRLDAWLDETYRDILKRQITTGASQLTPQIAL
                     YLYARSFYLKDKSVAEQHRTALDYFLDQARRYALSMRARQSQGHLALALNRFGDTETA
                     QAILKSLKEKSVTDPELGRYWRDPESTWWWHDAPVETQALMIEAFDEVGKDAAVVDGC
                     QVWLIKQKQTQDWRTTKATADAVYALLRRGRDLLSSDRLVEVNIGGLSITPSVPGTPT
                     RPDAPARSSVATGAPEPGTGFYERRFAGAEIKPSFGRITVRKQDAGVAWGSVHWQYLE
                     DIAKVQPHERTPLKLVKTLYTKRNTINGPVLEAVRGPLAVGDELVVRIELRVDRDIEF
                     VHLKDQRGSGTEPVNVLSGYRYQDGMGYYESTRDTASHFFIDRLRAGTYVFEYSMRIQ
                     LRGEYESGLATVECMYAPEFNSHSESVKLVVK"
     gene            7705..8613
                     /gene="gluQRS"
                     /locus_tag="PKK20_06135"
     CDS             7705..8613
                     /gene="gluQRS"
                     /locus_tag="PKK20_06135"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNU99497.1"
                     /translation="MTTPRGRGSYRGRLAPSPTGYLHLGHARTFWIAQRRAQARGGEL
                     VLRNEDLDRSRCRAAYVSAMFEDLLWFGLRWNEGPNCGGPYGPYAQSQRLTMYRDALE
                     RLQRLGHVYPCRCSRRDVLSALAAPHAEDDEPIYPGTCRPPHGRLWMGMPVVADSQPP
                     GAGRGRGAAGVNWRFLVPEGEAIEFVDGCCGPRRFTAGVDFGDFVVWRQDDVPSYQLA
                     CVVDDAAMKITEVVRGEDLLRSTARQLLLYRALGVAPPAFFHCPLLADAQGVRLAKRH
                     DALSLRTLRGQGAAPEYWHRQWETMV"
     gene            8760..>8964
                     /locus_tag="PKK20_06140"
     CDS             8760..>8964
                     /locus_tag="PKK20_06140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNU99498.1"
                     /translation="MRLTPTHIIALVAAAVYVIDQATKLAVLRVLDLADYHVVIDGFF
                     KFVHWGNTGAAWSLFYGNNGLLAL"
BASE COUNT         1457 a         2924 c         2941 g         1642 t
ORIGIN      
        1 catgggatca aggatgaaat ccacagcctt gcctgtgaga ggattgtgcg ccttcttggt
       61 gagcggcggg caatcgtaga agaagatacg gtagagttca cgtttaccgt ccggctgttt
      121 caagtgctcc cgacacatcc agtgcaggtc cgttgccgtc ttgttactgt tctgctggcc
      181 aacgaggcgc cgatagcgtc ggaggaagaa gtcgccgtca atgaggattg cggtagccat
      241 agtcacaaat gcgaagcccc ccgggctcgg caaccccaca atttacaggg ggcgcttact
      301 gccgaggggc tgtttagctc gttggagcgt tcagctccga aatccgaacc aaacataggc
      361 cgataacggt ttatgccaag cccaatttct tcagcacctt ctcgagttcg cgattggcga
      421 cttccatttc ttcctcggct ttgcgcagtt cgaccacggc ttcgtccagc ggcagcactt
      481 cagcctgttc gcccaccgaa acgtagcggc tgggcgacag gttgaagtcg ttcttcgccg
      541 cgtcggcgtt ggtgatgatg gccgagaggc cgtccacggc gcaccatttc aggtaggcat
      601 cggccagcgc tgtgccgagt gggtcttcga ggtagttctt ggggcggcct ttgctgaatt
      661 gcttcgaccc gttgatgatc gcgatctggc cggcgtgtct cttgcgccgg ttgaccacga
      721 ggatgatgcc cggcgcgggg agtgttgtga aacaggttct ccggcagcaa caccaccgcc
      781 tcgatgagat cggcctcgac gaacgccctg cgtatgtcgc gctccttgtt cgatccctgg
      841 ttgccgctcc cgcggctgac ggcgcagcta tcgagcacga ccgccatgcg cccgccgtcc
      901 gccagcgagg cggccatgtg ctgaatccaa aaccccaatc ggcgctggac gtggacggca
      961 ggccgcgcgt gaatcggccc caggtgtcgg tctctttgca gcctggaact gcgcgggacg
     1021 ccggttgcac ggctggggat cagcgaccgc cactggcctg agcacggtgc ttgtctcgag
     1081 caagggtctc gcggagctag accctccgag aaagattggc cagccctgcc tcacttgacg
     1141 accagcttca cactttccga atggctgttg aactcgggcg cgtacatgca ctccacggtc
     1201 gcaagcccgc tctcatactc gccgcgcagt tgaatgcgca tcgagtattc gaagacgtag
     1261 gtcccggcgc gcaggcggtc gatgaagaag tggctggcgg tatcgcgtgt cgattcgtag
     1321 tagcccatgc cgtcctggta ccggtagccg gagagaacat tgaccggctc cgtcccgctg
     1381 ccgcgctggt ctttcagatg cacgaactcg atgtcccgat ccacccgcaa ctcgattcga
     1441 accacgagtt cgtcgcccac ggccagtggg ccgcgaaccg cttcgagcac cgggccgttg
     1501 atcgtattgc gcttggtata gagcgtcttg acgagcttca acggcgtccg ctcgtgcggc
     1561 tggaccttgg cgatgtcttc gaggtattgc caatgaacgc tgccccaggc cacgcctgca
     1621 tcctgcttcc gcacggtgat cctgccaaac gacggcttga tctcggcccc cgcaaaccgg
     1681 cgttcgtaga aacccgtccc cggttcaggc gccccggttg ccacggacga tcgcgcgggg
     1741 gcgtcgggtc tggtcggggt tcccggaacg gacggcgtga tcgacaggcc gccgatgttc
     1801 acctcgacca accgatcgct cgacagcagg tcgcgtccgc gccgcaagag cgcgtagacg
     1861 gcgtccgccg tcgccttcgt ggtgcgccag tcctgagttt gtttttgctt gatgagccag
     1921 acctggcaac cgtccacgac ggcggcgtcc ttgcccacct cgtcgaaggc ctcgatcatc
     1981 aacgcctggg tctcgaccgg ggcgtcgtgc caccaccagg ttgattcggg gtcgcgccag
     2041 tagcgtccga gctcggggtc ggtgacggac ttctccttca acgatttcag gatcgcctgg
     2101 gcggtctccg tgtcgccgaa ccggttgaga gcgagcgcga ggtgtccctg agattggcgc
     2161 gcgcgcatcg acagcgcata gcgcctggcc tggtcgagga agtagtccaa cgccgtccgg
     2221 tgttgctccg ccacggactt gtctttgagg tagaagctgc gggcgtacag atagagagcg
     2281 atctgcgggg tgagctggct ggcccccgtg gtgatctgcc gcttgagaat gtcgcgatag
     2341 gtctcgtcga gccacgcatc gagccggtcc agcgcccgga cggcgggggc gacatcgaca
     2401 tccacgccga ggtgacggag gcgcgcgaac ccggcgacga tgtagagcgt gatgtagtcg
     2461 ctgcggctgc cgccggggaa ccagggccag gttccatcgt ccaactgcgc ttcccggacc
     2521 ttgtcgaggg cgcgggcctg ttcgctgtcg agccggttct cgtcaaagag cacgccgaca
     2581 ttgcggcgcg cctcgctttc cttttgggcc tcgcagaccc agggcgattc ctcgagcatc
     2641 acgcctttga ggtcctggtt cttttcgagc gggctgtcga gcgccggcgt ggccttccat
     2701 tgatcgaaga tgcgccggat tttcggattc gagtttgcga tgaaccgggc gagcgcgttg
     2761 gcgtagtacc ggttgaagac ttgctcgttg cactcgtaag ggaactccat caggtacggc
     2821 agggccagga ccgcgtacca ggccgggttc gacaccatct gcaccgtgag actctggtgg
     2881 cggagcgttc tcgatttgcc cgcatccagc agcgccgtga actcgaaacg gttcgtgccc
     2941 ggcccgacga tcggcagcgg gagcgactcg gtcaccggaa tccgccgcgc caacaccggg
     3001 accaggcctt cctcgccgtc ggagaccgtg ccggccgtcg caacggcctt gtacgtgagc
     3061 accgaaagcc cctcgggcac cgtcaagcgc cacgccacag aggtcgaggc cccgcccgcc
     3121 aagtcgaacg aacgctccga attcctgttg ccgagcaacg cgtcggccgg ctggttgtcg
     3181 aagccgttgt tgaaagtgag ccggaccttg ccgcgttggg gcttgtcgcc cgagttcgtg
     3241 accttgaccg tgaactcgac cacatcgccc tcgcgcagga accgcggcgg gttcggctga
     3301 accatcaggt ccttggccgt caccgccttg gcctcgatga agccggaacg aagcgcgcga
     3361 tcgtgggcaa aagccatgac acgccactcg gtcaacgcct ccggcatcgt aaagctcagg
     3421 cgcaccacac cctgcgcgtc ggccacaagg tgcggataaa agaacgccgt ttcgttcagg
     3481 ttccgccgcg ccgacacgag attcaaatcc gccgcggctg cgcccgcccc ctcgacggct
     3541 gcgccacccg ccgccccggc tccgtcgtcc ggggccgcct tgaggccgta gcgcatcagc
     3601 atggtcatct cggccgccgc ttcacccggc ggcgacatgg gaggggctgg aactgcggcg
     3661 ggcaggctca acgattcaat cgcgccagcg gccaccatac ctcgacctcg acgcgtgggc
     3721 agaccgcctg ccctcatgag catcgtcgca gcggggaatc cccagccggt ctggatcagg
     3781 tccggcggaa acgcccgata gccctcgagc accgcctcga atgacaccgg ccaatcctgg
     3841 cgaaacacgc ccaggccgcg cgcctgattc ccaaacgccg cctgacgccc cgtccactct
     3901 tggcgaaaca ccgaaaaccg gttcggccag tgcaatggaa ggaacgcgtc gagcgaacgg
     3961 tcgtagagcg tcgccaccag ttccggcagc cgcttcgagt tcgacgcatt gttcacaccc
     4021 accgcctccg ccgacggagc tgcgggcttg atgacgaggc tccacgtctc cttcttgcct
     4081 ggctcgagtt tcgatgtgaa atgctcccat tcgagcgtca ggttcttgtt gcgccacggc
     4141 accgcgatct gccgggtttc gagatacgcc cggttctcac ggacctgggt gacgcgcagc
     4201 gtgacgccgc cgcgcatggc ctcgtcgatc ggttgccgga tcagggcctg tgtctcgccc
     4261 ggagaggtcc agtacttgcg cagcgtccgg ccgcgagcct cgatttcgac atacgcgcgt
     4321 ccctcgccgt accccgtgcc ccaaagggcc gtgaactcgg acccgggttc gacggtccaa
     4381 tccgccgcct gcaagaagct gggcaccttg atggggaacg ccttgtccgt gggacggacg
     4441 acggtcagcg gaaggcgccc cgtgaccttc ttgccgtaac gatcctgcgt ctcgaggacg
     4501 gcgcggtacg ccccggcggg cagcttgact ttcgcggacg attccccctt ggcgtcggtg
     4561 gtgaaacctc cctcccacac cgcggcgccc atatcccaac ggtttggatt tgacaggtcg
     4621 gcgcccggag cgcccgcggc ggcgccatcg tctccgtctt cgtcaccgcc ccactgtttg
     4681 aacaaggagg gtcggctgac ccgatccggg gccttgagcg catggactct gaggattccc
     4741 tcggcctgct gcggttcgcc atcgagcgtg gtggcgcgca ctcgcagttc gaccggttgc
     4801 tcgtcggtga gccaatcgtc ggccgtcaac tgcgcttcga gggcggtgta cccgaggcgg
     4861 acagcgcgct cgtccgagcg ggtctcgccc gccgaatccg ttacgtccgc atgcacgctg
     4921 aaaatgaagg tcggatcgtc ctcgaccggc acggccgcgt cgggacgcgc aacgaactcg
     4981 acggcgaatg ccccgtccgc ccccgtgcgc agggtgccgt gggcgatctc ctggctgacg
     5041 ccgggctgga agcccccccg ccgccacccg taccagcccc accaccaagg catgcgcacc
     5101 tcgcgtcgga cgcggtactt gacttcggcg ttgtccaccg ccgccccggt gtagctcatg
     5161 gcgtggccga cgacctcgag tgtgtcgccc agtttggcgc ctgtcttggg cgcatcgagc
     5221 gtgacttgaa acttcggacg cttgtactcc tcgacattga cccagcctgc ccccggcggc
     5281 ccctcgatcg cgcacaattc catctggccc gtcaaccgat cgcgcggcgc ggtgaaactg
     5341 ccgctgaacg atccgtagtc gttggcgcga tgctgctggc gggcgatctc cttgcgattg
     5401 ccgtcgcgga acacgacggt cagcgtccgg ccggccaggg tttcgtaggt gtccttatcc
     5461 tgatcggccc gcaggcaaat gcccttgtac tgaaccgtct ggcccggacg gtagagggcg
     5521 cgatcggtga agaagagcgt ttgctcgaac ggacgccgct gcggggtgcg gccccagccc
     5581 gtgaattggt cggcgctggc gatcgcttgt ccgtttgcgg tggccttcag cacgtaattc
     5641 cgttgctgct cgcccaacgc cagggcgaac cgcccgtcct gatccgtggt cagggccggg
     5701 accgggacgc gttggccctg gcgatcgaga taccaggcct ggacttgggc gccgcggatc
     5761 ggctcgcccg acccggcttc gagcacgaat ccatcgagtt ggctcgcgtc ctggcggaga
     5821 acgagcgcga gatcgctgac ccaaatgtcc gtcatcgcaa gctggttgtc tttttcagcg
     5881 aaatcgggtc tgtggctggc aatcagaaag tagaaacccg gcttcagatc gtccgggacc
     5941 gtcacctcga ccacgtgctc gagaaagtcg gttgtcggcg gcagaggacg ggaccaggtg
     6001 taggccgccg ggcgcgccag gattgcggcg cgttcatcat cgcgcaggtt ctcgggccgg
     6061 ctccggctcc ggtcgaggtg gtcgtcccag ttcgccgata tgaggcggaa atgcgcctgg
     6121 tccacattcc ggtagcgcac ctcgatcgca ggccagggcc ggttccagat ccgctcggtt
     6181 cggatcgagg ccgacttcga ctcgatttcg gcgacgaggt tcgcggcgag ctttcccccg
     6241 gcgcttttcg ggtgaatctg ccgggcccgg accgcgagct gatgcgcctg ggcgaggtcg
     6301 ttctgctcgc gcagcgcctg ggcccagtgc cacagggcca tcgaggccag ctcgtggtcc
     6361 ccgtgggtcc cggcgaattg tttcatcgcg gcttggaacc gcgccgcctt cccctcgccg
     6421 acggcgttgt tcttggccca gaccaatcgc gccaggtcca gatcgagaaa ggcggtctga
     6481 tccgcgtcgc cggcgtggaa ccgcaagagg gcctggtaga gccggatcgc cttgatcagg
     6541 ggggacacgg gctcctgtgt cgaaccgccc ccgacagtcg ggtcccagtt caggaacgca
     6601 ttcaccggcc cgagggccgg gctgtcggcg gcaacctgga aggcgtcctg ggggagcgcg
     6661 cccgcctgtt cgccggacgt gtagaaggag agcgcttcgt gcgcaatgaa atcaaacaag
     6721 gtcgggcgat agacgtccgg gagcgctccg cgcggcagca agtcgtcata ggcgctgatc
     6781 cggattgtgc gaagctccgt ctcggcgctc agggcgattg tgaaccggcg gtcgatttcg
     6841 gcaaacaggc ggggcaagtc ccacgtctcg aaatcctcgc ccggagcctc ggctgtcgcg
     6901 gtgcgctgca tgaaccgcca gcggttcttg cgaaagtact gccagtacca atgcgccagg
     6961 atcgtctgca ggatcggctt gatctccgcc ggcgcgcccg agatctcggc gtccagccgc
     7021 cggatattct cccccggttt gcccgcctgc gcctccccat cgagcgcaat gcgccggacg
     7081 atggctttga cagcctccgc ccaggcacgg tcggccagcg cgccctggat gatcggggtc
     7141 aattccttga ccgcggtctg gggcaggccc ttttcgatcg cttcatccac gcgtttccac
     7201 tgggcgtctc gcgacgcggc gtgtagactg ggcttcataa gcaacacgac gagcaggaca
     7261 agggcatggc atttcatgac tcaggttctt cgagacagtg cacccgacaa gcccggaggg
     7321 caaccacccg attagacggc gccaggcggt cgatgctccc ggagatgttc gatttgggga
     7381 gacgattccg cgactgccgc agagtccctg gctcgagaca atgccggtga ccgagcaggg
     7441 cctcgtggaa ctcggccctc cgagagtggc ggttcatggg cagcgttgac agtccacaag
     7501 tcaatgtgca cggggctcat gagccggaaa cgctcaacgc tcaaccggct gaatgcactc
     7561 acgtgttcgg attggacagc gcggatgcgc cgcgcgcaga ctcgcggcgg caagccatcc
     7621 gcgggccgga cccgagcccg acgctcctcg atgaacgccg gcgccgggcg gctgcgggca
     7681 aggttgatgg aactggattt ggcaatgaca acgcctcgag gtcgaggcag ctaccggggg
     7741 cgcctggcgc cgtcgccgac tggctacctc cacttgggac acgcccggac tttctggatc
     7801 gcgcagcggc gggctcaggc gcgcggcggc gagttggtgt tgcgcaatga agacctggac
     7861 cggagccggt gccgcgcggc ctatgtgtca gcgatgttcg aggacctgct ctggttcggc
     7921 ttgagatgga acgaggggcc gaactgcggc gggccgtatg gaccgtatgc gcagagccag
     7981 aggctgacca tgtatcgcga cgccttggaa cggttgcagc gcctcggcca tgtgtatccc
     8041 tgccgctgtt cgagacgcga tgtcctgtcg gcactcgcag cgccccatgc ggaggacgac
     8101 gaaccgatct accccggcac gtgccgccca ccgcacggcc ggttgtggat gggaatgccg
     8161 gtggtggccg actcgcaacc tcctggcgcg ggtcgagggc gcggggcagc gggagtgaac
     8221 tggcgcttcc tggtgcccga gggcgaagcg atcgagtttg tggacggttg ttgcggtccg
     8281 cgacggttca cggcgggcgt cgattttggc gacttcgtgg tctggcgcca ggatgacgtt
     8341 ccgtcctatc agttggcctg cgtggtcgat gacgccgcga tgaagatcac cgaggttgtg
     8401 cgcggcgagg accttctgcg gtccaccgcc cggcaactgt tgctctaccg ggccttgggc
     8461 gtggcgccgc cggcgttctt ccactgcccc ctcctcgccg atgcgcaagg cgtccggctc
     8521 gccaagcgtc acgacgcgct cagtctcagg accctgcgcg ggcagggcgc ggccccggag
     8581 tactggcacc ggcagtggga gacgatggtg tagcgaggcg cgggacagag gcggtggaga
     8641 agcctcgagc ccgtttgccg cgaacgacga gttgtcaact atttagatgc gaccccattg
     8701 ccctgtccgg aatcgaaggc ttgccaccga catcgggaac tggctgtaat cgagttgttg
     8761 tgcgcctgac gcccacccat atcattgcgc tggtcgccgc cgcggtgtac gtgatcgacc
     8821 aggcgaccaa actggccgtc ctgcgggtgt tggaccttgc cgattaccat gtggtgatcg
     8881 acgggttctt caaattcgtc cactggggca atacgggcgc cgcctggagc ctgttctacg
     8941 gcaacaacgg gttgctggcc ctgg
//
LOCUS       DAOQRO010000121         8883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7313_length_8883_cov_6.258836, whole genome shotgun sequence.
ACCESSION   DAOQRO010000121 DAOQRO010000000
VERSION     DAOQRO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7313_length_8883_cov_6.258836"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PKK20_06145"
     CDS             complement(<1..1797)
                     /locus_tag="PKK20_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99499.1"
                     /translation="MNVHIRNRRCAPPVGAVPRLLTLMKSPGSQTTAARSAVSVAALT
                     TAIALLAACAPPAHGCGPFFPNSLLADPDGAALAAPNAVFELELERMRLIETNRRARP
                     SKDYAQDTLAAELEDLRQALDRSGVSSEDRDRIVVNHRLEREKITLFTPRDAAEGKWS
                     DANAAFELRPAPTNDIRIAPDAMPRMAAGLPGEFADYFRGSVAWHLGDIAGARVPWLH
                     LLERPAAERHFKSTWATYMLGKSWEEENPGRAIAHFRKVRALADAGFADSLGLSVASL
                     GWEARLCRRAKRLGDAIELYLEQHAAGDPSAAVSLGFVASDALRGGFGALQPLARRPR
                     PQRVVTAYLIAGGHTAPAIDIDSPLKEGILKGWEAISTRIPAVPAPKRSWHTYREPVL
                     LWLEAVERADVRDVESAEQLALAAYQGGDIQAAERWLQRARSTPVTQWLRAKLLLRQG
                     NMPDAAALLAAICRQFPVVQSSEISAVGDESRLRPSHAESALAADRLSGKRLADNLNT
                     PAGSFETVPMNQQVMAEISLERLARRQFVEALDALLHSSPVYWMDVAYVAERVLTLPE
                     LVEYVDRHCPPSTPLPADLAPVSDADELSAFEA"
     gene            complement(1794..3065)
                     /locus_tag="PKK20_06150"
     CDS             complement(1794..3065)
                     /locus_tag="PKK20_06150"
                     /inference="COORDINATES: protein motif:HMM:NF022784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HNU99500.1"
                     /translation="MSGKFAIVSLGCAAAAWIAWLQWPRPAHRVEGPMPHEVYVWQRA
                     WTEPVVTAVTQETTQFSRIVALAAEVHWEKGEAKVVRVSLDHDALRQSRRPIGLAMRI
                     GTFPGPFASDDARARWLAGLAASLVGEAESRQLEIGELQIDFDCAESKLAGYALWLGA
                     IRRAVSPHPVVITALPSWLKRPDLRPLVRAADGFVLQVHSLERPHRIEDDLQLCDPLR
                     ARKAVERAARLGRPFRVALPTYGYVTAFDPGGNFVGLSAEGPARQWPEGTRTREVRAD
                     PSALSGLIRQWTDDRPERMQGVIWYRLPVRGDRLNWTPPTLAAVMAGHTPKADLRIRL
                     RRTDPRLVELDLCNLGTADHNAPVRIAVRWEGARRVAGDAFKGFDFRERSNTSLECWS
                     DSGMDRLAPGAARTVCWLRFDREPEVNAQLQ"
     gene            complement(3461..4003)
                     /gene="msrA"
                     /locus_tag="PKK20_06155"
     CDS             complement(3461..4003)
                     /gene="msrA"
                     /locus_tag="PKK20_06155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNU99501.1"
                     /translation="MKTQPPSPIASASEYATLGGGCFWCLEAVFERVPGVKSVVSGYA
                     GGHTENPTYKAVCGGDTGHAEVVQIQFDPRAITYEQVLDLFWQAHDPTTLNRQGPDVG
                     TQYRSVIYYHDESQRVAAEKSKAAAGKQFARPIVTEIQALPRFYRAEVYHQDYYRNNP
                     NQPYCRMIIRPKLDELRKGL"
     gene            complement(4607..6778)
                     /locus_tag="PKK20_06160"
     CDS             complement(4607..6778)
                     /locus_tag="PKK20_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99502.1"
                     /translation="MFGMHIEFLRAENVRHVAPSDCDFSDGRGALRRWTLLPASEASD
                     ALLRCLALASLGRRQTSVLAPKLGTMLGVRTEHPARLEYTLIYHAPQERGPHRPARRQ
                     SGWQIWADGHVAPLSRAAMRSARPGAAYSRPDLGRSHIGRLLIGYGRRVTPHAGTDQF
                     GIYPDQRVRRCAGLFDVRARVTDPLAFLQRLRQKSCYRAGRARAVLARLTVDLSEWLG
                     WDVADSLARPGELEARWKAASAARRTPAVVLLDIARHAYDATERSFDPNPLLQPGVVL
                     LAAADTWCETRGLARFLALLGTRFPNLQFVVTLSASARSGFPARLARERLPIPEVQPR
                     PRPVPVRRLAPSTVLLVDADSTLPNVALMKLSRHFKSQGRKVVLARGVRSLPMAGTVL
                     ASCVFGTAASAKRVGVLRDRFGDTLQLGGSGVDLHRRLAPEIEALAPDYALYPELGDR
                     AIGFLTRGCPMHCPFCIVPEKEGPPRRVGDLETLLQGRKKLILLDDNLLAHADALELL
                     EAMARRGLQVNFNQTLDLRRLTPEAAGLLRRIGSTNATFTRRTYYFSLNDARGLDLLR
                     RRYALLQPRPSDNIEFVCMYGFNTTLAEDVERFRFLRSLPGAYVFVQRYQPIPGGPPA
                     NLSRFFDDRADALISELVRILYRQNMKSMETYYRWLALQYAAQCGRIHHVLVETLFRY
                     NGRSRMAGFLHRLERLSAAHGHLAAGPWFPLFLSHEGKRRK"
     gene            6849..7091
                     /locus_tag="PKK20_06165"
     CDS             6849..7091
                     /locus_tag="PKK20_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNU99503.1"
                     /translation="MTCVGGDGNFLLNVRPMRTGEIEAHQAERLKEMGAWLASVKSVK
                     SVVREWSSEAMPEFEPPQLAPTRAPHEFSQAQSSRA"
     gene            7381..8685
                     /gene="tig"
                     /locus_tag="PKK20_06170"
     CDS             7381..8685
                     /gene="tig"
                     /locus_tag="PKK20_06170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNU99504.1"
                     /translation="MNVTIEHLSPCKKLVRVEIDAEATRARADAVLKEFQRKVRLPGF
                     RPGKTPRALLLKTFAQEINAEMKRRITSDAYREAIEQHKLRPITRPEIEETPWELGQP
                     FAFTATVETEADFELPDYRGLPVRRDNRTVTEEDLERALRMLREQKPVFVDLDRPVQP
                     GDFVVVNYTGTCEGKPITDIAPTARGLTEQKNFWLQIQTGSFIPGFTDPLVGASAGES
                     RAVTVTFPDDFVTRELVGKQAVYQVEIVQVKERRIPELDDAFAGSYGAENLEALRAGV
                     RQDLELELKEKQQRQVRDQLVATLLGRVSFELPEALVENETRNVVFDIVKANQERGVA
                     KEAIDERKDEIYSVANNSARERVKAMLLLNRIAEKEGITATSQELTQRVLELAARYQI
                     KPDKLAKQLQERGALPQIQRQIIEAKVLNALELYAKIEDGSG"
BASE COUNT         1665 a         2838 c         2893 g         1487 t
ORIGIN      
        1 ggcctcaaac gccgagagct cgtcggcatc ggacacaggg gcgaggtcag cgggcaaagg
       61 agtcgacggc ggacaatggc gatcgacgta ctcgaccaac tccggaagtg tgagaacccg
      121 ttccgccacg taggcgacgt ccatccagta gaccggggag gagtggagca gagcgtcgag
      181 ggcctcgacg aactggcgcc gggccagacg ctccagactg atctcggcca tcacctgctg
      241 gttcattggc acagtctcga aactgccggc cggtgtattc agattgtcgg ccaggcgttt
      301 gccgctcaag cgatcggcag ccaacgcgct ttcagcgtgg ctgggccgca gccgagactc
      361 gtcgccgact gcgctgattt cggacgattg cacgacaggg aactgtcggc agatggcggc
      421 gagcaaggcg gcggcgtccg gcatattgcc ctggcgcaag agcagcttcg cccgcaacca
      481 ctgggtcacc ggtgttgacc gcgcgcgctg cagccagcgt tcggcggctt ggatgtcacc
      541 gccctggtag gccgccaagg cgagttgctc ggcggattcg acgtcccgca cgtctgccct
      601 ctcgaccgcc tcgagccaga ggaggaccgg ttcccgatag gtgtgccacg atctcttggg
      661 agcgggaacg gcggggattc tggtcgagat ggcctcccag cccttcagga tgccttcctt
      721 gagcggcgaa tcgatgtcga tggcgggcgc ggtatgcccg ccggcgatca ggtaagccgt
      781 cacaacccgc tgcgggcgcg ggcgacgcgc caggggttgg agtgcgccga agcccccgcg
      841 caaggcatcc gaagccacga agcccagtga caccgcggcg gacgggtcac cagccgcatg
      901 ctgttcgaga taaagctcga tcgcatcgcc caatcgcttg gcccggcggc agaggcgcgc
      961 ttcccaaccc aggctggcca cgctcaaccc gagggagtcg gcaaagcccg cgtctgccag
     1021 agcgcgcacc tttcggaaat gggcgatcgc ccggcccgga ttctcctcct cccagctctt
     1081 gcccagcatg taggttgccc aggtggactt gaaatgacgc tccgcggcgg gccgttcgag
     1141 caaatgcagc cagggcaccc tcgcccccgc aatgtccccc aggtgccagg ccacgctccc
     1201 ccggaagtaa tcagcaaact cgcccggcaa acccgctgcc attcgaggca tggcgtccgg
     1261 cgcgattcgg atgtcgttcg tcggggcggg gcgcagctcg aaggcggcat tcgcgtcgct
     1321 ccatttgccc tcagccgcat cccgcggggt gaacagcgtg atcttctcgc gctcgagacg
     1381 gtggttgacg acgattcggt cccggtcttc ggatgagaca cccgatcgat cgagggcctg
     1441 gcggagatcc tcgagttcgg cggccagcgt gtcctgggca tagtccttgc tggggcgcgc
     1501 gcgccggttg gtttcgatca accgcatccg ctcgagttcg agttcaaaga ccgcgttcgg
     1561 agcggcaagg gccgcgccgt ccggatccgc cagcaaggag ttcggaaaaa atgggccgca
     1621 tccatgcgcc ggcggggcgc aggccgcgag aagcgcaatg gcggtcgtca gcgccgcgac
     1681 ggagactgcg ctgcgcgcgg ctgttgtctg tgatcccggc gatttcatga gtgtcaggag
     1741 gcggggaacg gcgcccactg gcggggcgca ccggcgattc cggatgtgca cattcactgg
     1801 agttgagcgt tcacctcagg ttcccgatca aaccgcagcc agcagacggt ccgcgccgcg
     1861 ccgggcgcca gacggtccat tccggaatcg ctccagcatt cgaggctggt gttcgagcgc
     1921 tcccggaaat cgaatccctt gaacgcgtct cccgctaccc gccgggcgcc ttcccagcgc
     1981 accgcgatcc gcacgggagc gttgtgatcc gccgtgccga ggttgcacaa atccagttcc
     2041 accagccgcg ggtccgtccg gcgcagacgg atgcgcaggt cggctttggg cgtgtggccg
     2101 gccatgacgg ccgccagggt ggggggcgtc cagttcaaac gatcgccgcg aaccggcagc
     2161 cggtaccaga tgacgccttg catccgctcc ggacggtcgt ccgtccattg ccggatcagc
     2221 ccggacaacg cggacggatc agcgcgcacc tctcgggttc gagtcccctc cggccactgc
     2281 cgcgcggggc cttcggcgct caggccaacg aaattgccgc cgggatcgaa cgcggtgacg
     2341 tagccatagg tgggcagagc gacacggaac ggccgaccca gccgcgctgc ccgctcgacc
     2401 gcctttcgcg cgcgcagagg atcgcaaagc tgcagatcat cctcgatccg atgcgggcgt
     2461 tcgagcgaat ggacctgaag cacaaaacca tccgccgcgc ggaccagcgg gcgcaggtca
     2521 gggcgcttca accaggacgg caaggccgtg atcaccaccg ggtggggcga cacggctcgt
     2581 cgaatcgcac cgagccagag tgcgtatccg gccagcttcg actcggcaca gtcaaagtcg
     2641 atttgcagtt cgccgatttc gagctgacgg ctttcggctt ccccgacaag cgaggcggca
     2701 agcccggcca accaccgggc ccgcgcgtcg tcggaagcaa acggacctgg aaacgtcccg
     2761 atccgcatcg ccaggccgat tgggcggcgg ctttgccgca acgcgtcatg gtccagggag
     2821 acgcgaacga ctttggcttc gcccttctcc cagtggacct cggccgccag cgccacgatg
     2881 cgggagaatt gggtggtttc ctgcgtcacg gccgtgacga cgggctcggt ccaggcgcgc
     2941 tgccagacgt agacctcgtg cggcatcggt ccctcgacgc gatgggccgg gcgcggccac
     3001 tggagccagg cgatccatgc agccgcggcg catcccaagc tgacgatggc gaatttgccg
     3061 gacatgcggg ctcaaccgtc gggtggcggc agcgcgattc accggcttcg gaacacagcc
     3121 agctccgaca aaccgatgtt gggcgatccg ggtttcactt cattcgaccc accgttatcg
     3181 gccccctcga gcgcttcgcg caggagaggc ggggcattga tgtcgacgct ctcagtcgcg
     3241 gccgcggtcc gggcgctcgg accaaccgcc tgggacaatg tcagcaataa aagcaagctt
     3301 tcaacgggca cagtgcaaag acggcttgcg agggcaggca tccgttttat ggggctttga
     3361 cattcaacag gtcaatgcgc attggaccca tcaaccagga aacgttcaac gcttaacttc
     3421 caacgctcaa ctctcagctc gtcgcttcat gggattccct tcacaacccc ttcctgagct
     3481 cgtcgagctt gggtcggatg atcatccgac agtaaggctg gttcgggttg ttgcgatagt
     3541 aatcctgatg atagacctcg gcacggtaaa atcgcggaag ggcctggatc tccgtaacaa
     3601 tcgggcgcgc gaactgtttg ccggccgccg ccttggattt ctcggccgcc acgcgctggg
     3661 actcatcgtg atagtaaatc actgagcgat actgggtccc cacgtcgggt ccctgccggt
     3721 tcagcgtggt cggatcgtgc gcctgccaga acaggtcgag cacttgctcg tacgtgatcg
     3781 cacgcggatc gaactggatt tgcacaacct ccgcatgccc ggtgtccccg ccgcacacag
     3841 ccttgtaggt cgggttctcc gtgtggcccc cggcatagcc gctcaccacg gatttcacgc
     3901 cgggcacacg ctcgaacacg gcttcaagac accagaaaca gccgcccccc agcgtggcgt
     3961 actcggaagc ggatgcgatc ggcgaaggcg gctgggtttt catggcgggt tggggcggct
     4021 cagcttgaat cgcgcacgcg gcgaagcagg ccaacatacc tcggaacggc gtgttcatcg
     4081 ttatccaaat tccagatgcg ctgcattgta gacgagaact ctcttttcta caaatgccta
     4141 tccgatatgg ggaggggcgt ccacagcgcc aattcatgaa gtcgaaaagg tcggagtggg
     4201 gacggctcgt cccccgccat gtcaagtgaa gacggcgtgt gtcggcgacg gggccgtggg
     4261 gcgtccaaaa gcgacaatcg agtgtctgaa ccggccttgt tgctctttgc gcccttcatc
     4321 catgatccgt cctgagttca tgagggatcc ttggcggcgg gacgcggatg tcgaccgagc
     4381 ctggcagacg tccaatgagc ccgcccatcc gtgtttgatc ggtgtttgat cggtgtttga
     4441 tcggtgtttc acccgtggct ctttgaatgc cgggtgctct gtgtgctctg cgtctctgtg
     4501 gttcaatccg ggacggtgac tgtcatcgga tgggattcac cacagagaca cagagaccac
     4561 agagggaaga gagcctcatg aaccgggccg gatcatgaag gacgggtcac tttctccgct
     4621 tcccttcatg tgataagaac agggggaacc acggaccagc ggcgaggtgg ccgtgggcgg
     4681 cagagagtct ctcgagtcgg tgcaggaatc ccgccatgcg cgagcggccg ttgtatcgga
     4741 acagcgtctc gaccaagacg tgatggatgc gcccgcactg tgcggcgtat tgaagagcca
     4801 gccagcgata gtacgtctcc atgctcttca tgttctggcg atagaggatt cgcaccaatt
     4861 cgctgatcag ggcgtcggcc cggtcgtcga agaagcggct gaggttcgcc ggcggaccgc
     4921 cggggatggg ctggtaacgc tggacgaaca catacgcccc gggcagcgag cgcagaaatc
     4981 gaaaccgttc gacatcttcc gccaacgtgg tgttgaaccc gtacatgcag acgaactcga
     5041 tgttgtccga agggcgcggt tggagcagag cgtaccgtcg gcgcagcaga tcgaggcctc
     5101 gcgcgtcatt gagactgaaa tagtatgtgc ggcgagtgaa cgtcgcgttg gtggagccaa
     5161 tccggcgcag caaaccggcc gcctcgggcg tgagccgccg caaatcgagg gtctggttga
     5221 agttgacctg caaaccgcgg cgcgccatcg cctcgagcaa ctcgagggcg tcggcgtgag
     5281 ccagcaggtt gtcgtcgagc aggatcagct tcttgcgacc ttggagcaga gtttccagat
     5341 cgccgacccg gcgcggcggt ccctccttct cgggcacgat gcagaaggga caatgcatgg
     5401 gacaccctcg tgtcaggaac ccgatggcgc ggtcaccgag ttccggatac agcgcgtagt
     5461 ctggggccag agcctcgatt tccggtgcca ggcgtcggtg gagatcgacg cccgaaccgc
     5521 ccagttgcag agtgtcgccg aaccgatccc gcagcacccc gactcgcttc gccgacgccg
     5581 cggtgccgaa cacacaactg gccagcaccg tcccggccat gggaagcgac cgcacgccac
     5641 gcgccaatac caccttccgg ccttgggact tgaaatgccg gctgagcttc atcaaagcca
     5701 cgttcggcag agtgctgtcc gcgtccacca gcaacacggt cgaaggggcc agccggcgaa
     5761 cggggaccgg ccttgggcgc ggttggacct cgggaatcgg gaggcgttcc cgcgccaatc
     5821 gcgccgggaa accgctgcgg gccgatgccg agagggtgac gacaaactgc aggttgggaa
     5881 accgtgtgcc cagcaaagcg agaaagcgcg ccaggccgcg ggtttcgcac caggtgtccg
     5941 ccgcggccag caacaccacg ccgggctgca gaagcgggtt cgggtcgaac gaccgttccg
     6001 tggcgtcgta agcgtgcctg gcgatgtcga gaagcacgac cgccggcgtc cgtcgcgctg
     6061 cagacgcggc tttccagcgc gcttccaact cgccgggtcg agcgaggctg tcggcgacat
     6121 cccagccgag ccactctgac aggtccacag tcagccgggc gagcacggcg cgcgcgcgcc
     6181 ccgcgcgata acaactcttc tggcgaaggc gctgaaggaa ggccagcggg tccgtgacgc
     6241 gcgcccggac atcgaacaat ccggcacaac gcctcacccg ctggtccggg tagatgccga
     6301 actggtccgt gccagcgtgg ggcgtgaccc gacgcccata gccgatcaac agccgcccga
     6361 tgtgcgacct gccgagatcc ggacggctgt acgccgctcc aggtctggct gagcgcatgg
     6421 cggcccgcga taggggggca acatggccat ctgcccagat ttgccatcct gactgccgcc
     6481 gcgcggggcg gtggggtccc cgctcttgcg gggcgtggta gatgagcgtg tactcgagtc
     6541 gcgccggatg ctcggtacgc acgcccagca ttgtgccgag tttgggcgcc aacacggacg
     6601 tttgtcgccg ccccagactt gccagggcca agcaacgcaa gagcgcgtcc gacgcttcac
     6661 tcgccggcaa gagcgtccac cggcggagcg cgccccgccc atcgctgaaa tcgcagtcgg
     6721 atggcgccac atggcgcaca ttctccgcgc ggaggaactc gatgtgcata ccgaacatcg
     6781 aagatgaggc tcgatccgaa ccacagaggc aagccgaatg gctgcgtcaa gcctgaggcg
     6841 cgcaggcgat cacctgcgtc ggcggcgacg gcaattttct cctcaacgtc aggccgatgc
     6901 ggactgggga aatcgaggcg caccaagccg aacggttgaa ggagatgggc gcttggctcg
     6961 catccgtgaa atccgtgaaa tccgtggtca gggaatggtc ttcagaagct atgccagagt
     7021 tcgagccacc gcaactcgct ccaacgcgtg ctccacacga gtttagccaa gcgcagtcct
     7081 cgcgtgcgta atgttcttca gggccttcct cgtctggtgc gccatcgccg tgacagaggc
     7141 ttccacgcca ttctccgact tcgccggcgc aaccgccggg tcggcgacca ccggctccgg
     7201 cgggtggttc gtcctgacgt gcgtcgggtt ctcggctttg agtttcttga agagaactgc
     7261 tcctccgcag cgtgcttgac ggttgagggg ggcgtgctta gcctctgccg cttttaaagt
     7321 ggcgggggcg gcccgcgggc cggcggtgtt gccgcgaggc cgaacgaaag gctttttgaa
     7381 gtgaacgtaa ccatcgaaca tctcagtccc tgcaagaaac tcgtgcgcgt ggaaatcgac
     7441 gccgaggcca cccgggccag ggcggacgca gtgctcaagg agttccagcg caaggtgcgg
     7501 ctgcccgggt tccggcccgg caagacgccc cgcgcgctcc ttctcaagac ctttgcccag
     7561 gagatcaacg ccgagatgaa acgccggatc accagcgacg cctaccgcga ggccatcgaa
     7621 cagcacaaac tccgcccgat cactcgccct gaaatcgagg agactccctg ggaactcgga
     7681 cagcccttcg ctttcaccgc cacggtcgag accgaggcgg atttcgaact cccggattac
     7741 cgcgggttgc cggttcgccg ggacaaccgc accgtcacgg aggaggatct cgagcgcgca
     7801 ttgcgcatgc tccgcgagca gaagccggtc ttcgtcgatc tggatcgacc cgtccagccg
     7861 ggcgacttcg tcgtcgtcaa ttacaccggc acgtgcgagg gcaagccgat cacggacatc
     7921 gctcccaccg cgcgcgggct gaccgagcag aagaacttct ggcttcaaat ccaaaccggc
     7981 tcgttcattc cgggattcac cgatcccttg gtgggcgcaa gcgcgggcga aagccgcgcc
     8041 gtcacggtca ctttcccgga cgatttcgtc acgcgcgaac tggtgggcaa gcaggccgtt
     8101 taccaggtcg agatcgtcca ggtgaaggaa aggcgcatcc cggagctgga cgacgccttt
     8161 gcgggcagct acggggctga gaatctcgaa gccctccgtg ccggcgtgcg gcaggatctc
     8221 gaactcgaac tcaaggaaaa gcaacagcgc caggtccgcg atcagttggt ggccacgctc
     8281 ctgggccgcg tgagcttcga attgcccgaa gcccttgtcg agaacgagac gcgcaatgtc
     8341 gtgttcgaca tcgtcaaggc caaccaggag cggggtgtgg ccaaggaggc catcgacgag
     8401 cgcaaggacg aaatctacag tgtcgccaac aacagcgcca gggaacgggt caaggcgatg
     8461 ttgctgctga accggatcgc cgagaaggag ggcatcacgg cgacaagcca ggaactcacc
     8521 cagcgggtcc tcgaactggc ggcgcgttac cagatcaagc cggacaagct ggccaagcaa
     8581 ctccaggagc gcggcgcgtt gccccagatc caacgccaga tcatcgaggc caaggtcctc
     8641 aacgcgctcg agttgtacgc caaaatcgag gatggctccg gctagcccgg ttatttcaca
     8701 cgcttcgcga ggaaccgtaa ccgggctagc cggcaaaacc gtgcccggaa atggaccaca
     8761 agcattctgt tggccctgaa ggcgttcttc gataggaaca gtgtgttgca tatctctgtc
     8821 gctcgagtct ggcgcggttt tgcttctcta gcccgcatag cagtcctctt gcgaagtctg
     8881 agt
//
LOCUS       DAOQRO010000122         8819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7396_length_8819_cov_4.320059, whole genome shotgun sequence.
ACCESSION   DAOQRO010000122 DAOQRO010000000
VERSION     DAOQRO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7396_length_8819_cov_4.320059"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2461
                     /locus_tag="PKK20_06175"
     CDS             <1..2461
                     /locus_tag="PKK20_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNU99505.1"
                     /translation="GARDASGLVSLHLREGRNLLGMLHIKLRSNVAASPEELQALEGL
                     AGNVALRIARLRAGRQHRQAKNAIIDTLRRFHASLQATPLVAIQRVDRAGLVRYWNEA
                     STGLYGFAPERALNRRLSDLLLEGEEAAAFDRLVQSAWDTGHGAAPADWRVACADGSR
                     RCMSASLIPIREEGRVVDMFCMEVDVTGRRRAEKALRDSEALYRNLVDAAPLAVLVHQ
                     DGMVRFANAAALELLGANQPDELCWRPLASLVHPDAQERLEDLSGDGPRRPVELELVR
                     LDGGVVDAEVACLALHWENQPAEQWLIRDRTLTNRIQWELEAAREKLARIFQSSPAAI
                     LICSLPDHRLIEANPAFWRLIGYERKELLGRTLAELEIWGEDQPALMPASPRQARRMP
                     SFEVPLRTRAGDLRHMLISVEDFDFGDQRSRLLIMIDVTERKRLESDLRESQKLEAMG
                     LLARGVAHDLNNILTVIQGHQSLIPLGSRLSPQAQTSLAGISEAVAQASELTRQLLTF
                     SRKRQFEARRVDINELIGGVARLLRPTLGKQIQLELECAPGLGLVWADPGMIEQVILN
                     LGANARDAMPQGGRLTFTTELRFVRRPPASLQPAAAGGDYLCLTVRDTGRGIARENLG
                     RVFEPFFTTKAVAKGTGLGLATVRHIVEQHQGWIEVDSAEGRGTTFRVFLPAVAKSEV
                     AAPKSKLAPAPEGARQTILLVEDEPAVRDLARQVLERAGYRVLAAKNGTSATRLWRRH
                     GAAVRLLIADLVLPGGLDGGELAQRLQAERPDLRVILSTGYGMDFDDGRWQALAAARL
                     LPKPFSPDALARYVRECLEES"
     gene            2469..3284
                     /locus_tag="PKK20_06180"
     CDS             2469..3284
                     /locus_tag="PKK20_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNU99506.1"
                     /translation="MLHFQPIDSFDPPELAAYHTMRLPHKHFVERIFVAEGEKVVRRL
                     LESNLDVLSALMPAHHAEAMRPLLEARAPSIRVYTAERPLLERLAGYSMYQGVLAVGR
                     MPEPLALETAWQRSGRPRLFVAVDGLANAENLGAVVRNAVAFGTHALIVGETSSSPYL
                     RRAVRSSMGTVFDLPVVETNDLCAALAQLRTKGMRCVAAHPHTDRRTIAGTDLARDCC
                     LVFGNEGDGIAPAVRAACDECVAIPMPPGVDSLNVASAAAAFLFEANRQRGRM"
     gene            3404..4036
                     /locus_tag="PKK20_06185"
     CDS             3404..4036
                     /locus_tag="PKK20_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99507.1"
                     /translation="MGGPEIVLKGATTYTARVTDTAHGTIRSVEHAIQQLDDVVAALT
                     RDLADSRKRLAETQARIWAFFEYAERLAELARRQQDHTNIALMLRPIRSERFEGAPFH
                     RPCRAMSKTPVSPHQIPNKETTHLQTVTSRDGEHWSAVRDVGVPVNPNHGPQRTASGR
                     LIIAGNISFPYTDDPSGLAGWRMTGIYLYVIISRQKEAVQVSSVALVGLD"
     gene            4147..5460
                     /locus_tag="PKK20_06190"
     CDS             4147..5460
                     /locus_tag="PKK20_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNU99508.1"
                     /translation="MNVRSISRRRLLRQATLASATGALVGMVPSPVLRAAEPTRAPSP
                     RKDGLSVLEFGAAGDGRQDDTPAFETALKAAAQSGGNVVRIPRGNYLIRGTLDVPENV
                     VLEGYFRAPTARTQTQGSTLLALAGAGKADGQPFITLHQNSMLCGLTIFYPEQKQTNP
                     PVPYPWTVRGIGDNCSLVDVLLVNPYQAVDFGTYPAGRHCIRGLYAQALYRGLFIDQC
                     FDVGRVTDVHFWPFWGGWEGELGKFTREEGIAFVIGRTDWEYLQNCFCIGYRIGYQFV
                     KTKAGAPNAVLTQCGSDIGPTAVRVENVQDHAGISFVNGQFMAGIEIAETNKGPVKFT
                     ACGFWNIPTTDRHAVLEGKGHTTFNGCHFIGWGQKDREAPCVHARRGGLTVTACDFMD
                     RGKVQVAIEPEVDAALIYGNRLRGQERILNQAGDRAQIGMNVISG"
     gene            complement(5592..5933)
                     /locus_tag="PKK20_06195"
     CDS             complement(5592..5933)
                     /locus_tag="PKK20_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HNU99509.1"
                     /translation="MTPIHKLLNRIRWDKAFGQGRFEIGFWDRREGVIHRVALQDVAF
                     PESGEHTFQFVDETGRHRRVPFHRIREVYRDGELIWQRRTAGRHGAADGPLDGAFVPV
                     SLAQSKRGRSA"
     gene            complement(5971..6723)
                     /locus_tag="PKK20_06200"
     CDS             complement(5971..6723)
                     /locus_tag="PKK20_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNU99510.1"
                     /translation="MSNRKLPPGFVRHCKAVSAKRPKTVIDHILKHGFITTQELKDRY
                     GYNHPPRAVRDVKEHGIPIEMFRVEGSDGRKIAAYRFGDPAKARPGQQVGRTVLGREL
                     KSNLVALHGERCAIYLEGFPERELQVDHRIPFEVLGDVPGTEPNPDDYMLLSGSANRA
                     KSWSCEHCINWLELKKPEICRNCYWAFPDSYSHVAMREARRADILWTGLEVESYERLK
                     RRTLELQKNIPAYVKEIIEQHLKSEAVKKSVR"
     gene            complement(6720..7631)
                     /locus_tag="PKK20_06205"
     CDS             complement(6720..7631)
                     /locus_tag="PKK20_06205"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HNU99511.1"
                     /translation="MRVPHPIPYQGSKRNLAPAILTYFPDRIGTLIEPFAGSAALTLA
                     AAARGLAARYAINDLNMPLIHLWRAIIEAPEKLARQYETLWRAQHEDRRQYYDQVRDK
                     FNRTGRPDCFLYLLARCVKASVRYNANGEFNQSPDNRRLGALPSTMRDHILGASRLLH
                     GKTECSTLDYKEVVAQATADDVVYMDPPYQGVCGERDPRYLKGVVFDEFVEVLESLNL
                     RNIKYLVSYDGRTGERVHGRKLPERLRLHLIELDAGRSSQATLLGRDEITVESLYLSP
                     ALAEAGQLRRPYPHRAAEQLTLMERKK"
     gene            complement(8022..8264)
                     /locus_tag="PKK20_06210"
     CDS             complement(8022..8264)
                     /locus_tag="PKK20_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99512.1"
                     /translation="MAMSWGALFHYDFTNANLLVLLDCDPHLRYVREDIYARCRPAIE
                     RRMGVPLSEGMAAEIMLLATGGGGFSSGRTLGLAVF"
     gene            complement(8298..>8819)
                     /locus_tag="PKK20_06215"
     CDS             complement(8298..>8819)
                     /locus_tag="PKK20_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99513.1"
                     /translation="DTDHIWGVGGNAGWVWKSFLRGHNPIFMDPCDGSVLGEDRGWES
                     VRAAMGYARRVADRLNLAAMTPRNELASTGYCLACPGQEYLVYQPAGGKEFSLQLASG
                     EYQLEWIDPSRPELAGSDRLSAEDGSRQFQAPFAGSAVLHLKARSQAGVAQERAPVHA
                     VVDLGHQFTFYAE"
BASE COUNT         1547 a         2863 c         2804 g         1605 t
ORIGIN      
        1 gggggcgcgc gacgcctccg gtttggtgag tctgcatttg agggaagggc gtaatctgtt
       61 ggggatgctg cacatcaagt tgcggagcaa cgtggcggcc agccccgagg aactgcaggc
      121 gctcgaaggc ctggcgggga acgtggcgct ccgcatcgcc cggctgcgcg ccggcaggca
      181 gcatcgccag gctaagaacg ccatcatcga caccctgcgt cggtttcatg cctcgttgca
      241 ggcgacgccg ctggtcgcga ttcaacgagt ggatcgtgcc ggcctggtgc ggtactggaa
      301 tgaggcctcg accggcctgt acgggttcgc tccggagcgc gccctcaacc gcaggttgtc
      361 cgacctgctg ctggaagggg aggaagccgc ggcgttcgat cggctcgtgc agtcggcttg
      421 ggacaccggg cacggcgcgg cgccggcgga ttggagagtg gcctgtgcgg acggcagccg
      481 ccggtgcatg agcgcgagcc tcatcccgat tcgcgaggag ggccgggtgg tggatatgtt
      541 ctgcatggag gtggatgtca ccgggcgccg ccgggccgag aaggccctgc gcgacagcga
      601 ggcgctctac cgcaatctgg tggacgcagc gccgctggct gttttggtgc atcaggatgg
      661 catggtccgg tttgccaacg cggctgcgct cgaattgctc ggagcaaacc aacccgacga
      721 actgtgctgg cgccccctcg cgagcctggt gcacccggac gcccaggagc ggctcgagga
      781 tctgtctggc gacggtcccc gccgtcccgt ggaactcgaa ttggtacgcc tcgatggcgg
      841 tgtggtggac gccgaggtcg cctgcctcgc gcttcattgg gagaaccagc cggccgaaca
      901 gtggctcatc cgcgaccgca cactcaccaa ccgaatccag tgggaactcg aagcggcgcg
      961 cgagaagctc gcccgcatct tccagtccag tccagccgca atcctcatct gttcgctccc
     1021 cgatcaccgc ctgatcgagg ccaaccccgc cttctggcgg ctcatcggct atgagcgcaa
     1081 ggaactgctc ggccggactc tggcggaact cgaaatttgg ggcgaagatc aaccggccct
     1141 catgcccgcc tcgcctcggc aggcgcgcag aatgcccagc ttcgaggtcc cgctccggac
     1201 gcgcgccggc gatctgcggc acatgctcat ctctgtggag gatttcgact ttggcgatca
     1261 gcggagccgt ctgctgatca tgatcgacgt caccgagcgg aagcgtctcg agtccgatct
     1321 gcgcgagtcg cagaaactcg aagcgatggg ccttttggct cgcggtgtcg cgcacgacct
     1381 caacaatatc ctcaccgtca tccagggcca ccagagcctc atcccgctgg gcagccgcct
     1441 gtcgccgcag gcgcagacct ccctggccgg catcagcgag gccgtggctc aggcctcgga
     1501 actgacgcgc cagttgctca ccttcagtcg caagcgccag ttcgaagccc ggcgcgtgga
     1561 tatcaacgag ttgatcgggg gcgtcgcccg gcttctgcgc cccacgctcg gcaaacaaat
     1621 ccagctcgaa ctcgaatgcg cgccggggct ggggttggtc tgggccgacc cgggcatgat
     1681 cgaacaggtg atcctcaacc tcggcgcgaa cgcgcgcgac gcgatgcccc agggtgggcg
     1741 gttgacgttc acgaccgaac tgcgcttcgt gcgcaggccg ccggcaagcc tgcagcccgc
     1801 agccgcgggc ggcgattacc tctgtctgac cgtccgcgac accggacggg ggattgcgcg
     1861 ggagaatctc gggcgggtgt tcgagccgtt ctttacaacc aaggctgtcg ccaaagggac
     1921 ggggctgggt ttggccaccg tgcgtcacat cgtcgagcaa catcagggct ggatcgaggt
     1981 cgattccgcc gaggggcgtg gaacgacgtt tcgggtgttt ctgccggccg tggccaaatc
     2041 agaggtcgcg gcgcccaaat cgaaacttgc cccggcaccc gagggtgccc gccagacgat
     2101 cctgcttgtc gaggacgaac ctgccgttcg cgatctggcc cggcaggtcc tggaacgagc
     2161 cggttaccgg gtgctggcgg ccaagaacgg aacgtcagcc acccggcttt ggcgcagaca
     2221 cggagccgcc gttcgcttgc tgattgccga tctggttctg ccgggcggtt tggacggcgg
     2281 cgagctggcg cagcgtctcc aggcggagcg ccccgacctg cgggtgatcc tgagcaccgg
     2341 gtatgggatg gatttcgacg atgggagatg gcaggcgctg gccgcagcac gactgttgcc
     2401 gaagccgttt tcaccagatg ccctggcccg ctacgtgcgc gagtgcctcg aggaatcttg
     2461 aggccaccat gctccacttc cagcccattg attcgttcga tccgccggag ttggcggcct
     2521 atcacaccat gcgcctgccg cacaagcatt tcgtcgagcg catctttgtc gccgaaggcg
     2581 agaaggtggt gcggcggctt ctcgagagca atctcgacgt gctttcggcc ctgatgcccg
     2641 cgcatcacgc ggaggcgatg cgacctctgc tcgaagcgcg cgcgccctcc attcgagtct
     2701 acaccgccga gcgccctctg ctcgagcgcc tggccggata ctcgatgtat cagggcgtgc
     2761 tggcggtcgg gcggatgcct gaaccgctgg cgctcgagac tgcctggcaa cgcagcgggc
     2821 gccccagact ctttgtggcc gtggatggac tggccaatgc ggagaatctg ggcgcggtcg
     2881 tccggaacgc cgtcgctttt ggcacgcacg cgctgattgt gggcgagact tcgagcagcc
     2941 cctatttgcg gcgtgccgtt cgcagctcga tgggcaccgt tttcgacctc cccgtggtcg
     3001 agacaaacga cctgtgcgcc gcgctggcgc agctgcgcac caaaggcatg cgctgtgtcg
     3061 ccgcgcatcc gcacaccgac cggcgcacca tcgccgggac cgatcttgcc cgggactgct
     3121 gcctggtctt cggcaacgaa ggcgatggaa tcgcccccgc agtgcgcgcg gcgtgcgacg
     3181 aatgcgtcgc catccccatg ccgccgggcg ttgactccct caatgtggcc agcgccgccg
     3241 ccgcgtttct gttcgaggcc aatcgccagc ggggacggat gtaggccggg ctgcgcacac
     3301 gcggacgcgg ctcagtgcgg atcgcgatcg cagagcgaga ggagggcctc gggagtacgg
     3361 ttgaggtggt ccagccttcc aggtcttcgt cgccgacacc ttcatgggcg gacctgaaat
     3421 cgtcctcaag ggtgcgacca cctacactgc gagggtcact gataccgccc atggcaccat
     3481 ccggtcggtc gagcacgcaa tccagcagct cgatgacgtc gtcgcggctc tgacccgcga
     3541 cctcgccgac agccgcaagc gtttagcgga aacccaggcc cgaatctggg cgtttttcga
     3601 gtacgccgaa cgattggccg agttggctcg ccgccagcag gaccacacga acattgcgct
     3661 gatgctccgc ccgatccgct cggagcgctt tgaaggggcg ccctttcatc ggccttgcag
     3721 ggcaatgtcc aaaacgccag tctcaccaca ccagatcccg aacaaggaga cgacccatct
     3781 ccagacggtt acgagccgcg atggtgagca ttggagcgcg gtgcgggatg tcggggtgcc
     3841 cgtcaacccc aaccacggcc cccagcgcac agcctcgggt cgactgatca tcgccgggaa
     3901 catctcgttt ccctacaccg atgatccgag cgggcttgcc ggctggcgca tgacgggcat
     3961 ctatctctac gtcatcattt cgcgccaaaa ggaagcggtc caagtgtcga gcgttgccct
     4021 ggtgggtctc gattgaggtc cgtcgaatgc ccaggcgggc tcccgggtcg acgccgcaat
     4081 cgttcgcgcc ctgtgcagcc gtagccaaga cttgatctcc gcctgtgcaa aggcaaagtc
     4141 agccccatga acgtgcgttc gatctcccgc cgccgcctgc tgcgtcaagc aaccctcgct
     4201 tccgccaccg gcgccctggt cggcatggtg ccatcgccgg tcctccgggc cgctgaaccg
     4261 acccgggccc cttccccccg aaaggatggc ttgagcgtct tggagttcgg ggccgcgggc
     4321 gacggccgtc aagacgacac ccccgccttc gagactgcgc tcaaagccgc cgctcagtcc
     4381 ggcggcaatg tcgtccggat accgcgcggc aactacctga tccgaggcac gctggatgtg
     4441 ccggagaacg tcgtgctcga aggctacttc cgcgcgccga ccgcccggac tcagactcaa
     4501 ggcagcacac tgctcgcgct cgccggggcg ggcaaggccg acggccagcc gttcatcacc
     4561 ctgcaccaga actcgatgct ctgcggcctg accatcttct accccgagca gaagcagacc
     4621 aacccgcccg tcccgtaccc ctggacggtc cgcggcatcg gcgacaactg ctcactggtg
     4681 gatgtgctgt tggtcaaccc gtaccaagcc gtggatttcg ggacctatcc cgcgggccgg
     4741 cattgcatcc gcgggctcta cgctcaggcg ctgtaccgtg gactgttcat tgaccagtgc
     4801 ttcgatgttg gccgggtcac cgatgtccat ttctggccgt tctggggcgg atgggaggga
     4861 gagctgggga aattcacccg cgaagagggc atcgcgtttg ttattggtcg gacggactgg
     4921 gagtacctgc aaaactgctt ctgcatcggc taccggattg gctaccagtt tgtgaagacc
     4981 aaagccgggg cgcccaacgc agtgctcacg caatgcggtt cggatatcgg gccaacggcg
     5041 gtgcgcgtcg agaacgttca ggaccacgca ggaatatctt tcgtcaacgg tcagttcatg
     5101 gcggggatcg agatagccga gaccaacaaa ggcccggtga aattcaccgc atgcggcttc
     5161 tggaacatcc ccaccacgga ccggcatgca gtgttggagg gcaagggcca cacgacgttc
     5221 aacgggtgtc acttcattgg ctggggacag aaggaccggg aggctccgtg cgtgcacgcg
     5281 cgacgaggcg gcctgacggt gacggcgtgc gatttcatgg accgaggcaa agtccaggtg
     5341 gcgatcgagc cggaggttga tgcggccctc atctacggga atcggctgcg gggacaggaa
     5401 cggattctca accaggcggg tgatcgtgcc cagatcggca tgaacgtgat ttcaggctga
     5461 ctacctcagc gggcaaccac gccagggtgt gaacggcgcg ggcttcggac ccgtctcgct
     5521 gaccggattc cgattcgaga tgcgggattt gacttcatcg gccgtctgtc gccggtttgt
     5581 ccgaagttct ttcacgctga tctaccgcgt ttggactggg caagactcac agggacaaaa
     5641 gccccatcca aaggaccatc ggcagcacca tgccgaccag cggttcgtcg ttgccagata
     5701 agttcaccgt ctcggtaaac ctcgcgaatc cggtgaaaag gaacacggcg atgccgcccg
     5761 gtctcgtcca cgaactggaa cgtgtgctca cccgattcgg gaaacgcgac atcctgcaac
     5821 gcgacgcggt ggatgacgcc ttcgcgccgg tcccagaagc cgatctcaaa tcgtccctgg
     5881 ccaaaggcct tgtcccaccg gatgcgattg agcaatttgt ggatcggtgt catggcgagg
     5941 cgggtttctg gttcacaacc atttcctgaa ctatcgaacc gacttcttca cggcttccga
     6001 cttcaggtgc tgctcgatga tctccttcac ataggcgggg atgttcttct gtaattccag
     6061 cgtgcgcctc ttcagccgct cgtaggactc gacttccagg ccggtccaga ggatgtcggc
     6121 ccgccgcgcc tcgcgcatgg cgacatggga gtagctgtcg gggaaagccc agtagcagtt
     6181 gcggcaaatc tcaggtttct tcaactccaa ccagttgatg cagtgctcgc atgaccacga
     6241 cttagcccgg ttggccgaac cgctcagcag catgtagtcg tccggattcg gctccgtgcc
     6301 gggaacgtca cccagcactt caaaggggat gcgatggtca acctgcagtt cgcgctcagg
     6361 gaagccctcc aagtagatcg cgcagcgttc gccatgcagc gcaacaaggt tcgacttgag
     6421 ttccctgccc aacaccgtcc tacccacttg ctgaccaggc cgcgccttgg ccggatcgcc
     6481 aaagcggtac gcggcgatct tccgtccatc gctgccctcc acccggaaca tctcaatcgg
     6541 gatgccatgt tccttgacgt ctcgcacggc ccgcggcgga tggttgtagc catagcggtc
     6601 tttcagttcc tgcgtcgtga tgaagccgtg cttcagaatg tggtcaatga ccgtcttcgg
     6661 acgcttggcg ctgaccgcct tgcaatgtct cacgaaaccc ggcggcagct tgcgattgct
     6721 catttcttcc tttccatcag cgtcaactgc tcggcggcgc ggtgcggata cggccggcgc
     6781 aattgccccg cctcggccag tgccggcgac agatacagcg actccaccgt gatctcgtct
     6841 cgccccagta gcgtcgcctg cgacgaccgc ccagcatcca actcgatcag gtgtagccgc
     6901 agccgctctg ggagcttgcg tccgtggacg cgctcgccag tgcgcccgtc gtagctgacg
     6961 aggtacttga tgttccggag attcagcgac tccagcactt ccacaaactc gtcgaacact
     7021 acgcccttca ggtagcgcgg gtcgcgctca ccgcagacgc cttgatacgg cgggtccatg
     7081 taaaccacat catccgccgt cgcttgggcg acgacttcct tgtagtcaag tgtcgaacac
     7141 tcggtcttcc cgtgcagcag gcgcgacgcc cccaggatgt gatcgcgcat cgtgctcggc
     7201 agcgcgccca agcgacggtt gtccgggctt tgattgaact cgccgttcgc gttgtaacgc
     7261 accgacgcct tcacgcagcg agccagcaga taaaggaaac agtcgggccg gcccgttcgg
     7321 ttgaacttgt cgcgcacctg gtcgtagtac tgccgccggt cctcatgttg tgcccgccag
     7381 agcgtctcgt attgccgcgc caatttctct ggtgcctcga tgatggcccg ccacagatgg
     7441 atgagcggca tgttcaggtc gttgatggcg tagcgcgcgg ccaaaccgcg tgccgccgcc
     7501 gccagcgtga gtgccgccga tccggcaaac ggctcgatca gcgtgccgat ccgatctggg
     7561 aaataggtta gaatggccgg cgcgagattc cgcttgctgc cctggtacgg gatggggtgg
     7621 ggaacgcgca tgggttcagg cagtgcagtt cctaatagtc ccgcggtcct gtttcaccac
     7681 gcccgcaccg aacgaaccag tgtggtcctc cggcttcttc gagagtcgct cgacggtcat
     7741 gcggcagcgt tggtttcgat tgtctgacaa caacgcaaac ggtaacggcc tgaacgatgc
     7801 gccgcaagcc cgtaagacaa agcaggcggc gtgagtgagt tcggtcccgc caccaccagc
     7861 ttgcccttct cgcttagccg gatcagtcgc atcccttctc tcaccaagcg gggcttccga
     7921 ttctgcgcga atcactgcca caaaagctgc tggcgattgc tcggagtcgt gactggcgcg
     7981 gtgttgccgc gatcctaact atgctcgacg ctccagccgc ttcagaacac ggccaagcca
     8041 agggtccgac cggaggagaa gccgccgccg cccgtcgcca gcagcataat ctcggcggcc
     8101 atgccttcgg aaagcgggac gcccatgcgt cgctcgatcg cgggccggca acgcgcgtag
     8161 atgtcctccc tcacgtaacg cagatgcgga tcgcagtcca gcaggacgag caggttggcg
     8221 ttcgtgaaat cgtagtggaa cagcgccccc caactcatcg ccatcggttg atcgggcagg
     8281 tattgccgat gaaagcgtca ctcggcgtag aacgtgaact ggtgacccaa gtccacgacg
     8341 gcatggaccg gtgcgcgttc ctgcgccact cccgcctgac tccgggcttt gagatggagg
     8401 acggcgcttc cggcgaaggg agcttggaac tggcggctgc catcctcggc actcaggcgg
     8461 tcgctccccg ccagttcggg ccgggaggga tcgatccatt cgagttggta ttctcccgat
     8521 gccaattgga gggaaaactc ttttccgccc gccggttgat agacgaggta ctcctggccc
     8581 gggcaagcca agcaatagcc ggtggatgcg agttcgtttc ggggggtcat ggccgccagg
     8641 ttcagccgat cggcaacgcg gcgagcgtag cccatggcgg cgcggacgga ttcccagccc
     8701 cggtcctcgc ccagcaccga gccgtcgcaa gggtccatga agatgggatt gtgtccgcgc
     8761 aaaaagctct tccataccca gccggcgttt ccgccaacac cccagatgtg atcggtgtc
//
LOCUS       DAOQRO010000123         8732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7520_length_8732_cov_5.620721, whole genome shotgun sequence.
ACCESSION   DAOQRO010000123 DAOQRO010000000
VERSION     DAOQRO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7520_length_8732_cov_5.620721"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKK20_06220"
     CDS             <1..292
                     /locus_tag="PKK20_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99514.1"
                     /translation="RLNRTLCSGLRFDVRWKARSSPTAKLKLRLELVTSTGSRERPLA
                     LETPVRPKSFGGCWARLALEGEAFRNAGEVIAWRATLWNGDQLEDEHRSFLW"
     gene            448..2526
                     /locus_tag="PKK20_06225"
     CDS             448..2526
                     /locus_tag="PKK20_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNU99515.1"
                     /translation="MQATTVSIPWIKVVLLCLLACPPIRGAGRTIAYGDPPRSPRPGE
                     DWRGARRVALAHSGGAGFAIGLSSREESRIAYNTLYAAGEYADSAWDGSVSTCVAGTT
                     ASTFKELTLLRVNYFRAMAGVPATVVLDDTFNSKNQAAALIMAANDMLSHTPSAEWHC
                     YTAAGQEAAGNSNLALGLSGPDAISGYIQDDGANNYPVGHRRWVLYPQTRLMGTGDIP
                     ASNAWKAANANWILDGHFFDPRPATRDGFVAWPPPGFVPYPIVFPRWSLSYGNADFSG
                     ATVSMSDGGVPLAIVVEPIETGMGENTLVWYPAALDPTSASAWPRPAVDTTYAVAISN
                     VLVAGKPTSFSYSVTVFDPQVAGPDTQWATVTGPNPAALNQPNLYSISPLSRATGYQW
                     LCARPAALNVIESADSGLGGFTARTSPDYDLLVTVPSYSGKFYRLAHPAPTDQTLTYQ
                     RLLLPGAGAQVSFRSRLTLATSAQQAKVQISPDSGLSWRDVYTQAGTNDVAGLGELEF
                     VNRTVSLASFAGRPILLRFNYQVDPDRPYYVNTDVGVGWHFDNVKFTDTQELQNPVTA
                     PANADITFSFGPLETGDHVLCARAQYYSRYYGEWGVVSRVNAKNLPTPTIEISGPPRL
                     EGGKTIVEFQVTNYRSTLVFDLMRATNPAGPWQADSQAAIEGVSANTRFRLSSPSTAT
                     GPVFLRVRAR"
     gene            complement(2653..4155)
                     /locus_tag="PKK20_06230"
     CDS             complement(2653..4155)
                     /locus_tag="PKK20_06230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNU99516.1"
                     /translation="MKTSFKFVCILFAVSLNLRCAAAPDTALALPRSSPEQQGVSSSA
                     LLAFVDAADREIEGIHSFMLVRHGHVVAEGWWAPYNPQSPHELYSLSKSFTSTAVGLA
                     VAEGKLSVHDEVLKFFPEHAPAEPSANLKAMRVSDLLRMATGHQDEPVWRAGNLTVKL
                     FLDHPVPHKPGTHFKYNTAATFMLSAIVQKVTGETVLDYLRPRLFEPLGIEHPTWGAN
                     AEGITLGGYGLSVRTEDIARFGQLYLQKGQWGGKPLLPGAWVEAATGLQVATGSNPNS
                     DWDQGYGYQFWRCRHGAYRGDGAFGQYCVVVPRLDAVIAITGGLGDMQQVLNLVWDRL
                     LPALGAQPMPADRESQKALERRLAGLELPPAQGRPSSPMASTISGRRFVFAANERKLE
                     TIGLEVGTDGQGVTLVTRIGGVERRLACGKNAWTKARHPFGPYPEQPVCVSGAWTADD
                     TYVVKMCAYETPFVATFKLRFSGDQVTFDQAVNVSFGPTKQPQLTGRAER"
     gene            4657..6783
                     /locus_tag="PKK20_06235"
     CDS             4657..6783
                     /locus_tag="PKK20_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99517.1"
                     /translation="MSGAVMPTNMPAPAVLWKTNVAGIDRWAVGIRARGQRWVYDRIQ
                     PIWRPPASDWLRIKEAAQGDPIELVVAGAGPGKGEPPLARGSIQFAVSRWAVQTPLFY
                     REVNLPFADAVKDPSKIRWRFGALDCPTASPVVLENLPVCGNCHSFSRNGEFLAMDVD
                     YANSKASYVITRTAPEMRLATSDLITWNDYKPEDGRQTYGLLSQISPDGRYVLSTVKD
                     RSVFVPRPELAFSQLFFPLKGIVAVYDRQTRQFFSLPGADDPVFVQSNPTWSPDGQWV
                     VFARNRAYELEKTRDTGRILLTPEECEEFLERGREFRYDLYRVPFNGGAGGVAEPLRG
                     ASANGRSNFFPKYSPDGRWIVFCQAANYMLLQPDSALFIIPAEGGEPRRLGCNLGRMN
                     SWHTWSPDGRWLVFSSKEHSDYTQLYLALITENGEASPPVWLEHMVAPGRAANIPEFV
                     ALRPDAIGRIREQFLDDYSYTRAGNEFYRAGEADRAIEKFTLALAMNPDNAMAHQRLG
                     FLLFRVKNRLSDAIGHSRAAVRLEPQNGFAHFDLGAALAAQGDLTNAAVHLEESVRLL
                     PRGFDRQYNVVDMRLAWAEVCYRLERYAQTVEVLEPALERAHAHARANYLTAMARAWL
                     DETSTTTPFFERAVGSEPRLARLPDYHDLLSRNYVNQGLYAEGLDAAQTAHRLALAAG
                     RAQQAAKLERRIELCRSRLSGNAKDK"
     gene            7090..>8732
                     /locus_tag="PKK20_06240"
     CDS             7090..>8732
                     /locus_tag="PKK20_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99518.1"
                     /translation="MKRTTLIGLAASVFLLAGAIQAQTFTGIDLGSPLLSGSATDNGD
                     GTMTIVGGGADIWGTADSGFYYYTTMSDPVWDAVVRVRDLVNHDDAGTGTIGDDGWTK
                     CELMVRLPDEWAGMPMADDPFIANMTTRSGGQNQIGPQWRTARAGNADWNALGLTIRP
                     DYPNVWLKIERREALFTLYYGTDGATWNKYVDIDTSKSDLVGADNSTTFGTAWPAEVY
                     VGIAVTAHYDGDPTGGIATISDLKVTKLTTPPDLQIVTDVQDTSAVAGTAALFEFEAV
                     NAAIPNGYVSSYQWSRNGTDIPGATGSKLNIIVGPDDNGVKFKCRASFTGATLDSREA
                     TLTVTPGTLYSGYAKWEFFDNLSRGVVSSGNCGPANMIMTTTGFETVTDFSDTYASRL
                     SAWFYPPADGKYVFFISSDDDADLYLSTDASPANKRLIAQEAGWSPARSWVGIGGAPS
                     TVTQKRSDQWSPDDGETTPGAEGYDLLKDTPYYIEAIHHEGGGGDNLAVTYKLIDDPD
                     PVNGDAPRLVGSVIRYLSWAVTKLDVATQPVDATVFEEQL"
BASE COUNT         1583 a         2879 c         2664 g         1606 t
ORIGIN      
        1 gcgcctaaac cgcaccctgt gctcgggcct tcgtttcgac gtgcgatgga aagccaggtc
       61 gtcaccaacc gccaagctca aactgcgact cgagttggtg acctcgacgg ggagccgcga
      121 gcgtccgctg gcgctcgaaa cgccagtccg gccaaagtcc tttggggggt gttgggcgcg
      181 attggcgctc gagggtgagg cattccgcaa cgcaggcgag gtgatcgcgt ggcgcgccac
      241 tctgtggaac ggagaccaac tcgaggacga gcatcgctct ttcctgtggt gaatcgtcct
      301 tccgcatgtc gccgcgtctc ctttgcactg caatcgatga tcgtcctgga ttgcgtcagc
      361 agcggttccc agctcagaat ccgcgcggcg ggtggcctcg ggatgcagct ttgccgcgcg
      421 gtgtctcagt gaattgtcgt gccattcatg caagccacca ccgtctcgat cccttggatc
      481 aaagttgttc tcctctgtct gctggcctgt ccgccgatcc gcggcgcggg acggacgatt
      541 gcgtacggtg atccgccccg gtctccgcgg cccggcgagg attggcgagg cgcccggcga
      601 gtggcgctcg cccattcggg cggcgccggg ttcgcgatcg gtctgtccag ccgcgaggaa
      661 tcgcgcatcg cctacaacac cctctacgcc gccggggaat acgcggactc ggcgtgggat
      721 gggagcgtat cgacttgcgt ggccgggacg accgcctcga cgttcaagga gttgaccctg
      781 cttcgagtca attacttccg cgccatggca ggcgttccag cgactgtcgt tctggatgac
      841 acgttcaaca gcaagaacca ggcggcggct ctgatcatgg ccgccaatga catgctgagc
      901 cacacaccgt cagccgagtg gcattgctac accgccgccg gtcaggaggc ggcgggcaac
      961 tccaatctcg ccttgggatt gtcggggccg gatgccatca gcggttacat ccaagacgac
     1021 ggcgcaaaca actaccccgt cgggcaccgc cgctgggtgc tctaccccca gacgcggctg
     1081 atgggcacgg gcgacatacc cgcctcgaac gcgtggaaag ccgcgaacgc caactggatt
     1141 ctcgatgggc acttcttcga tccgcgtccg gccacgcggg acggctttgt cgcgtggcct
     1201 cctcccggtt tcgtgcccta tccgatcgtc ttcccgcgtt ggtccctgtc atacggcaat
     1261 gcggatttca gcggcgcgac cgtgagcatg agcgacggcg gtgtcccgct tgcgatcgtg
     1321 gtcgagccga tcgagaccgg catgggcgag aataccctgg tctggtatcc ggcggctctc
     1381 gacccaacct ccgcgtcggc ttggccgcgt cccgccgtcg acaccaccta cgcggtcgcg
     1441 atttcgaatg tgctggttgc gggcaaaccg acgagttttt cgtactcggt aacggtgttc
     1501 gacccccagg tggcaggacc agacacgcag tgggcgactg tcaccggccc caacccggcg
     1561 gcattgaatc agcccaacct ctactcgatc agccccctgt cgcgcgcaac cggctaccag
     1621 tggttgtgcg cacgccccgc ggcgctgaac gtgatcgaaa gtgccgatag cggtctgggc
     1681 ggtttcacgg cgaggaccag tccggactac gatctcctcg tcacggtgcc atcgtattct
     1741 ggcaagttct acaggctggc gcatccggcg ccgaccgacc agaccctcac gtatcagcgt
     1801 ctcctgctgc cgggcgcggg ggcgcaggtg tcgtttcgca gccggctgac cctggccacc
     1861 agcgcccagc aggccaaggt gcagatctca cccgattccg gcttgagctg gcgggacgtc
     1921 tacacccagg cgggcaccaa tgacgtcgcc ggcttgggcg aactcgagtt tgtcaaccgc
     1981 acagtgtccc tggcgtcgtt cgccggacgc cccattctgc tgcggtttaa ttaccaagtc
     2041 gaccccgacc gcccctacta cgtcaacacg gatgtcggcg ttgggtggca cttcgacaac
     2101 gtcaagttca ccgacaccca ggagctgcag aatcccgtga cagcgccggc caacgcggat
     2161 atcaccttca gcttcggccc actcgagacg ggcgatcacg ttctttgcgc ccgcgcgcag
     2221 tactacagca ggtactacgg cgaatgggga gtcgtcagca gggtcaacgc caagaacctg
     2281 cccacgccga caatagaaat ttccggtcca cctcgtctcg aaggcggcaa gacgatcgtc
     2341 gaattccagg tcacaaacta ccgatcgacg ctcgtgttcg atctcatgcg cgccaccaac
     2401 cccgccggcc cctggcaggc cgacagccag gcggcgatcg agggcgtctc cgccaacacg
     2461 cgcttccgcc tctcgagccc gtcgaccgcc accgggcccg tcttcctccg cgtgcgcgcg
     2521 cgctagcgca cgtttctcac caacttcgga agaacgcgga gccgatgtca tcgaaaggtg
     2581 cactcatgcg cgccagccaa gcgggtcttt caccaacgga cgggtgtact actctcaatt
     2641 tttacacaga ccctaacgct cggcccgccc ggtgagttgc ggttgtttcg tggggccgaa
     2701 cgagacattg actgcctgat cgaacgtcac ctggtcgccg gagaacctga gcttgaacgt
     2761 ggccacgaag ggcgtttcat aggcgcacat cttcacaaca taagtatcgt ccgccgtcca
     2821 ggcgccgctc acgcagaccg gttgttcggg ataaggaccg aacggatggc gcgccttcgt
     2881 ccacgcgttc tttccgcaag ccaggcggcg ttctaccccg ccgatccgag tcacgagcgt
     2941 gacgccctgg ccatcggttc cgacctcgag tccgatcgtc tccagcttcc gctcgttcgc
     3001 ggcgaagacg aatcgcctgc ctgagattgt tgacgccatc ggggaggaag gccggccctg
     3061 cgccggcggc agttcgagac ccgcaagcct gcgttcgagt gccttctgag attcgcgatc
     3121 ggcgggcatc ggctgcgcac ccaatgcagg cagaagccgg tcccagacca gattgaggac
     3181 ctgctgcatg tctcccaggc cgcccgtgat cgcaatcacc gcatcgaggc ggggcacaac
     3241 cacacagtac tggccgaatg cgccgtcgcc gcggtaggcg ccgtggcgac agcgccagaa
     3301 ctggtaaccg tagccctggt cccagtcgct gttcggattg ctgccggtcg ccacctggag
     3361 gccggtggcg gcctcgaccc acgcgcccgg gagcagaggc ttgccgcccc attgcccttt
     3421 ctgcaagtag agttggccga accgcgcgat atcctccgtc cgaacgctca atccatagcc
     3481 cccgagcgtg atcccctcgg cattggcgcc ccaagtcgga tgctcgatcc cgagcggctc
     3541 gaagagtcgg gggcgcaggt agtcgagcac ggtctctccc gtgacctttt gaacgatcgc
     3601 cgacaacatg aacgtggccg ccgtgttgta tttgaagtgc gtgcccggct tgtgaggcac
     3661 cgggtgatcg agaaaaagtt tgaccgtgag attgcccgcg cgccaaacag gttcgtcctg
     3721 atgccccgtc gccatgcgta acagatcgct tacgcgcatc gccttcaggt tggcgctcgg
     3781 ctcggcgggc gcatgctcgg gaaagaactt caggacttcg tcgtgcacgc tgagtttgcc
     3841 ctcggccacg gccagaccca ccgccgtcga agtgaaactc ttgctcagcg aatacagctc
     3901 gtgcgggctc tgcggattgt agggcgccca ccacccttcc gccaccacgt ggccatgccg
     3961 gaccagcatg aagctgtgga tgccctcgat ctcgcggtcg gccgcatcga cgaatgcgag
     4021 cagcgccgag gacgagacgc cttgctgctc ggggctgctg cgcggcaagg ccaaggccgt
     4081 gtcgggcgcc gcggcgcatc gcaggttcag ggaaacagcg aatagaatgc agacgaactt
     4141 gaatgaggtt ttcataatac gctcgacgtc gagcttgccc aaatccgggc cgtacgtctg
     4201 ccagaaaatg gttggtggtt gagcgttgag cgttgaaccg gggaggttct aatggcaatc
     4261 gtattcttgc tcgtaatcct ctgttgtcga ttacgattac gattacgagt gcgactgaga
     4321 gtgagagtgg gagtgggaat gggcaacttc aattcatggg cccaatgcgt tctcaaaagc
     4381 gaaacaccgc cctcagtcgc gatggcgagg gacgcggcgt cccctctcca gccttttgtt
     4441 cttcatgaat tggcgctgca gcgccgccga ttcgatttgt cttgtccgcg gattcctact
     4501 attctggcct ccgtgacgct gggtggcaaa aggtctctgg ccgcctttct gatcgcggcc
     4561 ggcgcattga gcgcatatcg cgggtcgtgc gatgacgatg cctggcctct gttgacaggg
     4621 ccgggtccat gcgcgcccat cgagattcga caccccatgt ctggcgcggt catgccgacc
     4681 aacatgcctg cccccgcggt gctgtggaag acgaacgtcg ccgggatcga ccgctgggcg
     4741 gttgggatcc gagccagggg gcaaaggtgg gtctacgacc gcatccagcc gatctggcgt
     4801 ccgccggcat cggactggct gcgcatcaaa gaggcggccc aaggcgatcc gatcgagttg
     4861 gttgtggccg gtgcggggcc cggcaagggc gagccgccgt tggcccgagg ctcgattcag
     4921 ttcgctgttt ctcgttgggc cgttcaaacg cccttgttct accgcgaggt gaatctgccg
     4981 ttcgccgacg cggtcaagga cccatcgaag attcgctggc ggttcggcgc gctcgactgc
     5041 cccaccgcgt ctccggtggt gctcgaaaac ctgccggtct gcggcaactg ccactcattc
     5101 agccggaacg gcgagttcct ggcgatggac gtcgactacg ccaacagcaa ggcctcctat
     5161 gtcattaccc ggacggctcc ggagatgcga ttggcgacga gcgatctgat cacctggaat
     5221 gactataagc cagaggacgg tcgccagacc tacggcctct tgtcgcaaat ctctcccgat
     5281 ggccgctacg tcctgagcac ggtcaaggac cgttccgtgt tcgtcccccg gccagagctg
     5341 gccttttcgc aactcttttt ccctctcaag ggcattgtgg cggtctatga ccggcaaacc
     5401 aggcagttct tctcgctgcc gggtgccgat gaccccgttt tcgtgcaaag caaccccacg
     5461 tggagccccg atggccagtg ggtggtgttc gcccggaatc gggcctacga actcgaaaag
     5521 acgcgcgaca cgggcaggat tctcctgacg ccggaggaat gcgaagagtt cctcgaacgc
     5581 ggtcgggagt ttcgatacga tctctatcgc gtcccgttca atggcggggc cggcggcgtc
     5641 gccgaaccgt tgcgcggggc ctcggccaac gggcgcagca acttctttcc caagtattcg
     5701 ccggatggcc ggtggattgt cttctgccag gctgcgaact acatgttgct ccagcccgac
     5761 agcgcgttgt tcatcatccc ggcggagggc ggcgagccca ggcggctcgg atgcaacctc
     5821 ggccgcatga actcgtggca cacatggtct ccggacggac gctggttggt cttctcgtcc
     5881 aaagagcact cggattacac ccagctctat ctggcgctca taacggagaa cggcgaggcc
     5941 agcccgccgg tctggctcga gcacatggtc gcccccggac gcgcggccaa catccccgag
     6001 ttcgttgcct tgcgtccgga cgcgatcggg cggattcggg agcagtttct tgacgactac
     6061 tcctacacac gcgcgggcaa cgagttctac cgggctggcg aagccgatcg cgcgatcgag
     6121 aagtttacac ttgcgctcgc gatgaacccg gacaacgcca tggcccatca acggctgggt
     6181 ttcctgctgt tccgggtcaa gaaccggctt tcggacgcga tcggccactc gcgcgccgcc
     6241 gtgcggctcg aaccccaaaa cgggttcgcc catttcgatt tgggggccgc cctggcggcc
     6301 cagggcgatc tgactaacgc cgccgttcac ctcgaggaat cggtgcgcct gctgccccgc
     6361 ggcttcgacc gccaatacaa cgtcgtcgat atgcgccttg cctgggccga ggtctgctac
     6421 cgactcgaac gctatgcgca gaccgtcgag gtgctcgaac cggccctcga acgcgcccac
     6481 gcccacgccc gcgcgaatta cctcacggcc atggcccgag cctggctcga cgaaacctcg
     6541 accaccaccc ccttcttcga acgcgcggtc gggtccgagc cgcgcctggc acgattgccc
     6601 gactaccacg acctcctcag ccggaattat gtcaaccaag ggctctacgc cgagggcctc
     6661 gacgccgccc agaccgcaca tcggctcgcc ctggccgccg gacgcgcgca acaggccgcg
     6721 aaactcgaac ggcgaatcga gttgtgccga agtcgcttgt ccggaaacgc gaaagacaaa
     6781 tgagggggca tgacggttga aaccgattcg tgtccctcgt ggtggatgcc tcttttccgt
     6841 gccccctgtt ttgcccgttt tttgcttgcc ctcccttgcg atttcgtcag agtgtgcgca
     6901 tggcaatccg caaggtcttc aacacctgta cggtgtcgtg tgccctatgc gcgaggaggg
     6961 agcgcgacgc attccggcac agcctgcgga aggttcattc gcagaccgca cgtgcgccgt
     7021 ccgggcatcg actcgggacg ggcacgacaa cggagtaacc acacaaacca acaccagaga
     7081 taagcaacca tgaaaagaac aacactcata ggcctggcgg cctcggtgtt cctgctcgcc
     7141 ggcgccatcc aggcccagac attcacgggc atagacctgg gctcgcccct gttgtccggc
     7201 tcggcaaccg acaacggcga cggcaccatg accatcgtcg gaggcggtgc ggacatctgg
     7261 ggcacggcag attcggggtt ctactactac accaccatga gcgatccggt ttgggacgcc
     7321 gtggtgcggg tccgcgacct cgtcaatcat gacgacgcag gcaccggcac gatcggtgac
     7381 gacggctgga ccaagtgcga actgatggtg cgtttgcccg acgaatgggc tggtatgccg
     7441 atggccgatg atcccttcat cgccaacatg acgacccgtt caggcggaca gaaccagatc
     7501 gggccccagt ggcgcacagc ccgcgccggc aacgccgatt ggaacgcgct gggcctgacc
     7561 atacgtccgg attaccccaa cgtctggctc aagatcgagc gacgcgaggc gctgttcacc
     7621 ctctattacg gcaccgacgg cgcgacttgg aacaagtacg tcgacattga cacttccaag
     7681 agcgatctgg tcggcgcgga caactcgacc acattcggca cggcctggcc ggcggaggtt
     7741 tatgtgggca tcgccgtcac cgcccactac gatggggacc cgacgggcgg catcgccacc
     7801 atctcggatc tcaaggtgac caagctcacc accccgcctg atctccagat cgtcacggat
     7861 gtccaggaca cttcggcggt tgccggcacg gcggccctgt tcgagtttga agccgtcaat
     7921 gccgcaatcc ccaacggcta cgtctcctca taccaatggt ctcgtaatgg aaccgacatc
     7981 cccggcgcga ccggatcgaa gctcaacata atcgtcggac ccgatgacaa cggcgtcaag
     8041 ttcaagtgcc gggcctcttt tactggcgcc acgctcgaca gccgcgaggc tactctcacc
     8101 gtcactccgg ggaccctcta ctccggctat gccaagtggg agttcttcga caaccttagt
     8161 cgcggagtcg tctcgtccgg caattgtggt ccggcgaaca tgatcatgac cacgaccggc
     8221 ttcgagacgg tgacggactt ctcggacacc tacgcctcga ggctgagcgc ctggttctac
     8281 ccgcctgccg acggcaagta tgtgttcttc atctcctccg acgacgatgc ggacctgtat
     8341 ctgagcaccg acgccagccc ggccaacaaa cggctgatcg cccaggaagc gggctggagt
     8401 ccggcgcgaa gttgggtcgg catcggggga gcgcccagca cggtcaccca gaaacgatcc
     8461 gatcagtggt cccccgatga cggcgagacc actccgggtg ccgagggcta cgacttgctc
     8521 aaagacacgc cgtattacat cgaggccatt caccacgaag gcggcggcgg cgacaacctc
     8581 gctgtcacat acaaactcat cgacgatcct gatccggtga acggcgacgc gccccgactg
     8641 gtgggctctg tgatccgtta cctgtcgtgg gcggtgacca agctcgatgt ggccactcaa
     8701 ccggtggatg ccacagtgtt cgaggaacaa ct
//
LOCUS       DAOQRO010000124         8709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7553_length_8709_cov_4.094176, whole genome shotgun sequence.
ACCESSION   DAOQRO010000124 DAOQRO010000000
VERSION     DAOQRO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7553_length_8709_cov_4.094176"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKK20_06245"
     CDS             complement(<1..1246)
                     /locus_tag="PKK20_06245"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNU99519.1"
                     /translation="MENHQKPAFGFVQNRVPWIVAGAALLLYIVTLNGWARLDSLLSL
                     SAAGGWSLYVTVRAPLLHLIAVPFRILPSGLQAPALNLLTAVMAALALALLARSVALL
                     PFDRTRETRLRELREYSLLSIPMAWLPVVLACVLSGLQLSFWEHATVFTGEMLDLLLF
                     AYVLRCLLEFRIDRRQVWLTKMAFVYGLAVANNYAMIAFFPGFLVALAWIKSWEFLNP
                     TFLLRTLAAGVAGLLLYLYLPLVNVLGGDQMGTFGQHVRAVLGSQLAALRSVPPYVIL
                     VLSFTSILPVLLLSIPWSSDNPSTSAAGASIIRFLVRALQIILVAACLSVFFDTRWSP
                     RQLGAVFGAVFLPLYYLSALSMGYFAGYFLLMTVPAKGRGWPQAPSLLERLGKTGPAF
                     AALVLVATLVLLGGRNWQPVAVG"
     gene            complement(1275..2498)
                     /locus_tag="PKK20_06250"
     CDS             complement(1275..2498)
                     /locus_tag="PKK20_06250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNU99520.1"
                     /translation="MTHSERPSTDLTVGSRHVLSIDDIAFGGAGVGRIGGLVVFVPFV
                     LPGEEVEAELIEVKKQYARARLLRVVRPAPQRVAPRCPYFGACGGCQYQHIDYAEQLR
                     IKHHHVADLLERIGKRPAGLVDPVIPCPQAYGYRNRLMVRSQWNKAEQRLVIGFLRAD
                     SRLVVDVELCAIADPALQDQLRHVRAHPPPRGGLKVVLRRMPADWEVPPDSFFQNNFH
                     LLPALLDVVRERLAGSGARWLVDAYCGVGFFAIELAGVVERFVGVEYDAMAVEAARRN
                     AARRGAANGEFVVGDATVCLGAISARFPPEETAVIVDPPRVGCRPEAIRSLLVSGPAQ
                     ILYVSCHPATLARDLNALCGGGVYELVQVTPLDMFPQTQHVECVTDLRRRHPAARGEG
                     ATSGSRQADNLANPD"
     gene            2659..3594
                     /locus_tag="PKK20_06255"
     CDS             2659..3594
                     /locus_tag="PKK20_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNU99521.1"
                     /translation="MAKPALGRGLDALLGGVPATRESSAPAAQTPISPDASAAAPGTE
                     RVQRVALNRIRPCPFQPRKDFPEEALRELADSIRAQGIVQPLIVRARGDGFELIAGER
                     RWRAAQLAGLAHVPVLVREADDTAALELALIENLQRENLNPLEEAQGYKQLLDLFNLR
                     QEDIASKVGKSRVAVANALRLLNLPGEVQAHLRSGRLSVGHAKVILGLRLPAEQTLAS
                     EQVIRNGLSVRQTEDLVARLGQAGMRQPGAASAPRDAVGPRDVHVVALESRLQERMGT
                     KVNLRYRQGRGTIEIRFHSEDELSRLLELLGVTAE"
     gene            3654..4793
                     /locus_tag="PKK20_06260"
     CDS             3654..4793
                     /locus_tag="PKK20_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNU99522.1"
                     /translation="MNPGYTPTHSQREDCARQLTAAAARVRALTAFVGLDGFVDEILH
                     VVDKRQSAEKYVRLAKMGQWGERISAASGRSTNIELVSLMTKLGGNGPIMANALAGLG
                     LRVTYLGLLGYPHLHPVFEEFSRRAKVHSIAEPGYTDAVEFEDGKVMVGKHQSLKQMT
                     WENIKARFGKREFASHFGGADLVGFVNWTMLPYMSEVWEALLEELCPKLRGPRRILFF
                     DLADPEKRLKKDLARALRLVVQFGRYFEVILGLNEKEAHEVAKALDIDAPAPTPQALS
                     DLGATIHRRVPVQTLIIHPVSYALAIGAGGLAITSGPTTKKPLITTGAGDHFNSGFCL
                     GRLLGLDTHESLLCGVTCSGFYVRTARSPGVNDLVRMLKDKTVKY"
     gene            4805..5425
                     /gene="def"
                     /locus_tag="PKK20_06265"
     CDS             4805..5425
                     /gene="def"
                     /locus_tag="PKK20_06265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNU99523.1"
                     /translation="MRLNVVNYGHPALRQRGARIEAVTPEIQSLAANMIETMHAAHGI
                     GLAGQQVAKPLQIFVLDLREVSDRPSTLEWEGEPANIADRMPMVLINPEVTPLDGITD
                     TGPEGCLSFPEIFAEKITRPASVRVTAWNERGEIIRFACGGLLARAVQHEHDHLRGIL
                     FIDRMTSAERRRWQPELDALQDATRTELQKGQIMQRESLGDWKRIV"
     gene            complement(5437..5970)
                     /locus_tag="PKK20_06270"
     CDS             complement(5437..5970)
                     /locus_tag="PKK20_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99524.1"
                     /translation="MHMFFPCLAAVYHPEEILCQRLVDQFLNSPGGDGQVACAGIMSC
                     NGIDGMPRIEPLCRFNNGRRLQIEGVVPDLVDIITREVALTNLELDDENNCALNEHDV
                     DAASHSGNVEFEEQSAAEAKQGRSQKIDPMEPSIALGRLNSPFAVRYQCAQHCLAACS
                     KERLHSIGVIRSGKELK"
     gene            6286..7668
                     /locus_tag="PKK20_06275"
     CDS             6286..7668
                     /locus_tag="PKK20_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNU99525.1"
                     /translation="MKQAVILAGGKGTRLRSVLGDLPKPLIPIGGRPLLEHQFELRRR
                     HGFNRVLVLAGHRAERIEAHCGDGSRWGLQIECEIEREPLGTAGAVLAAFSRLPERFL
                     VLYGDEMLNVDLDRIWRAHIAAGAAATLLLHPNDHPFDSDLVDVDDQDWIRAFHHRPH
                     PPEALFQNLVNAALYVVERSALSPWAGSNKALDFGQDLFPGMLDRGNRLLGYRSPEYI
                     KDIGTPDRYARVCREYEAGVVAASSLAVPQKAVFLDRDGTLNREVGGVCAPDRLELLP
                     GVPAAIRELNHHGWRVVVVTNQPVVAKGFCTEADIQRVHNKLETLLGAAHAFVDRIYW
                     CPHHPERGFPGERPDLKLDCACRKPKPGLVLRAAHDLNIDLRASWFVGDSTTDLATAS
                     NAGVRSILVRTGHAGRDGKFESRPDHVFETLTEAVQCIVSAGNARRVCSDTRGAAREF
                     EANGIQDSTS"
     gene            7683..8663
                     /locus_tag="PKK20_06280"
     CDS             7683..8663
                     /locus_tag="PKK20_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNU99526.1"
                     /translation="MIISRTPLRMSFVGGGSDLPAYYRLHGGAVVSTAIDKYVYVTVN
                     PKFDNNIRVSYARTEEVDEVARLRHPLVRESLRHLDIAGGVEITSIADIPSRGTGLGS
                     SSAFTVGLLHALHAWQGRYRSAEQLARESCLIEIERCGEPIGKQDQYAAAFGGFNFIE
                     FSADERVVVQPIVCRHETLERLQDNLVAFYTGITRPAAKVLREKQRRIAAESDKQAML
                     GRMVALARTLRDELQNNNLDAFGEVIHQNWVLKRQLSDDVSTGQIDAWYDAARGAGAV
                     GGKILGAGSGGFLLFYAPRRRHRAIRDALSELRPIPMRFEPQGSRIIFVH"
BASE COUNT         1694 a         2758 c         2817 g         1440 t
ORIGIN      
        1 ggccaaccgc cacaggctgc cagttccgcc cgcccaggag aaccagagtc gccaccagga
       61 ccaacgcagc gaacgcgggg cctgtcttgc ccagcctctc caaaaggctc ggagcctggg
      121 gccagccccg gcccttggcc ggcacggtca tgagcaggaa atagccggcg aagtagccca
      181 tgctcaacgc ggacaggtaa tagaggggta gaaacaccgc gccaaacacc gcgccgagct
      241 ggcgggggct ccagcgcgtg tcgaagaaca cggacaagca ggcggccacc aggatgatct
      301 gcagggcgcg caccaggaac cggatgatcg aggccccggc cgcgctggtg cttgggttgt
      361 cggacgacca cggaatgctg agcaggagca cggggagaat cgaggtgaat gagagcacca
      421 ggatgacgta tggcggcacc gaacgcagtg cggcaagctg gctgcccaga accgctcgca
      481 cgtgctgtcc aaacgtgccc atctgatcgc cgcccaggac gttgaccaat ggcagataga
      541 gataaagcag caaacccgcg acacccgcgg ccagggtccg caacaagaag gtcggattca
      601 ggaattccca ggacttgatc catgccaggg cgacaaggaa acccgggaag aaagcgatca
      661 tcgcgtagtt gttggccact gcgagtccat aaacgaacgc catcttggtc agccaaacct
      721 gtcgacgatc gatgcgaaac tcgagcaggc agcgcaagac ataggcgaag agcagcagat
      781 cgagcatctc gcccgtgaac acggtggcgt gctcccagaa gctgagctgc aaacccgaga
      841 gcacacaggc cagcacaacc ggcagccagg ccatcggaat gctcagcagc gagtactcgc
      901 gcagctcgcg aagacgcgtc tcgcgggtcc ggtcaaaggg cagcaaggca accgatcgcg
      961 ccagcaaggc gagtgccagg gcggccatga cggcggtgag caggttcaag gccggggcct
     1021 gcaagccgga ggggagaatc cggaacggga ccgcgatcaa gtggagcaac ggggcgcgca
     1081 cggtcacgta gaggctccat ccgcccgctg cgctcaagga gagcaggctg tcgagccgcg
     1141 cccagccatt gagggtgacg atgtagagca gcagggccgc gcccgccaca atccagggca
     1201 ctcgattctg cacgaatccg aaagccggtt tttgatggtt ctccatacgt gtagcgggca
     1261 gaggttgaac caactcagtc tggatttgca aggttatccg cttgtcgtga tccgcttgtc
     1321 gcgccctccc cccttgccgc ggggtggcgg cggcgcaggt cggtgacgca ctccacgtgc
     1381 tgcgtctgtg gaaacatgtc aagcggcgta acctgcacga gttcatagac cccgccaccg
     1441 cacagagcgt tcaagtcgcg ggcaagcgtc gccgggtggc acgacacata caaaatctgc
     1501 gccggccccg agacgagcag actgcggatg gcttccggtc ggcatcccac ccggggcgga
     1561 tccacgatca ccgccgtctc ctccggaggg aaccgggccg aaatggcgcc gaggcacacg
     1621 gtggcgtcgc cgaccacgaa ctcgccgttg gccgcgccgc ggcgtgctgc gttgcggcgc
     1681 gcggcctcga cggccatggc gtcatactcg acgcccacaa agcgctcgac gactccggcc
     1741 aactcgattg caaagaaccc gacaccgcag taggcgtcca ccagccaacg ggcgccgctg
     1801 ccggccaggc gctcgcgcac gacgtccagg agcgcgggca gcaggtgaaa gttgttttga
     1861 aaaaaggagt ccggcggcac ctcccagtcg gcaggcatcc tacgcaggac caccttgagc
     1921 ccgccacggg gcggcgggtg ggcgcggaca tggcggagtt ggtcctgcag cgcgggatcg
     1981 gcgatggcgc acaattcgac atccacgacc agccgactgt cggcgcgcag gaacccgatg
     2041 accaaccgct gctcagcctt gttccattgg ctgcgcacca tcaggcgatt gcgatagccg
     2101 taggcctggg gacagggaat caccgggtct accaggcccg ccggtctctt tccgattcgt
     2161 tcgagcaggt ctgccacgtg atggtgtttg atgcgcagtt gttccgcata atcgatgtgc
     2221 tggtactggc acccgccgca cgcgccaaag taagggcatc gaggcgcgac cctttgaggt
     2281 gcggggcgca caacgcgcag caagcgggca cgggcgtact gcttcttcac ctcgatcagc
     2341 tcggcttcga cctcctcgcc cggcaggacg aacggaacga agaccacgag tccgccgatg
     2401 cgccccacgc ctgcgccgcc aaaggcgatg tcgtcgatcg acaggacatg gcgcgatccg
     2461 acagtcaagt cggtgctggg ccgttcactg tgggtcatag ttcgaggcac gggtgctgcg
     2521 gacccggtgt tgaggcgcgt gcatggccca aggctcgccc gccggcagag ccccaggcaa
     2581 tccgaaaatc cgcgcggtcc gcggctcgat tgtccttcgc gttgacaagg cgatgttcgt
     2641 tggcaacact ccgcggtcat ggccaagccg gctctaggtc gcggcttgga cgcgctgctg
     2701 ggcggggttc ctgccacccg ggagtcgtcc gcccccgctg cccaaacgcc catcagtccg
     2761 gacgcttccg ccgccgctcc ggggacggag cgcgttcagc gcgtggcgtt gaaccgcatt
     2821 cgcccgtgcc cttttcagcc gcgcaaggat tttccggaag aggcgctgcg cgagctggcc
     2881 gattcgattc gcgcgcaagg catcgtccag ccgttgattg tccgggcgcg cggtgatggc
     2941 ttcgagttga ttgcgggcga acggaggtgg cgtgcggcac aactcgctgg gctggcgcat
     3001 gtcccggtgc tggtccgcga ggcggacgac accgcggcgc tcgaactggc attgatcgag
     3061 aaccttcagc gcgagaacct gaatccgctc gaagaggcgc aaggatacaa gcaattgctc
     3121 gatctgttta atctccgcca ggaggacatc gcatccaagg tcggcaagag ccgcgtggcg
     3181 gtggccaacg cgctgcgtct cctgaacctt ccgggcgagg tgcaggcgca tttgcggagc
     3241 ggacgccttt cggtcggcca cgccaaggtg atcctgggtc tgcgcctgcc ggcggagcaa
     3301 acgctggcgt ccgaacaggt cattcgaaat ggtctgagcg tgcgccagac ggaggacctg
     3361 gtggcgcgcc tcgggcaagc cgggatgcga cagcctggag cggcatcggc gccgcgggac
     3421 gccgtcgggc cgcgggatgt tcacgtcgtg gctctggaga gccgactgca ggaacgcatg
     3481 ggaacaaagg tgaacctccg ctacaggcag ggccggggca ccatcgaaat ccgcttccac
     3541 agcgaagacg aactgagccg gttactggag ttgctcggcg tgacagccga gtgagcgcat
     3601 gccgccggcc gaccgcagcc tgaaatccga ctttcgatct ggagaaagga cgcatgaacc
     3661 cgggatacac accaacgcac tcgcagcgcg aagactgcgc ccgacaactg actgcggccg
     3721 cggcgcgcgt ccgcgccctg accgcgtttg tcggtctgga tggtttcgtg gatgaaatcc
     3781 tgcacgtggt ggacaagcgg cagagcgcgg agaaatacgt gcgcctggcc aagatggggc
     3841 aatggggaga gcgcatcagc gcggcatccg gccggagcac gaacatcgag ttggttagcc
     3901 tgatgaccaa actgggaggg aacggcccga tcatggccaa cgccctcgcc ggcctggggt
     3961 tgcgcgtgac gtacctgggc ctgctcggtt acccgcatct gcaccccgtg ttcgaggaat
     4021 tctcgaggcg cgccaaagtt cactcgatcg ccgagccggg ctatacggat gcggtcgagt
     4081 tcgaggatgg caaagtgatg gtcggcaagc accaatcgct gaagcagatg acctgggaga
     4141 acatcaaggc ccgcttcgga aaacgcgagt tcgccagcca tttcgggggc gccgacctgg
     4201 tggggttcgt caactggacc atgctgcctt acatgagcga ggtctgggaa gctttgctcg
     4261 aagaactttg cccgaagctc cgcggaccgc gccgcattct gttcttcgat ctggccgacc
     4321 cggagaaacg gctcaagaag gacctggcgc gggcgctcag gctcgttgtc cagttcggca
     4381 ggtatttcga ggtcattctc gggctgaatg agaaagaggc tcacgaggtg gccaaagcgc
     4441 tcgacatcga cgcgccggcg cccacgcccc aggccctctc ggacctgggc gccacgatcc
     4501 atcgccgtgt cccggtccag acactcatca tccacccggt ctcctatgcc cttgccatcg
     4561 gagccggcgg gctggcgatc acatcggggc cgaccacgaa aaaacccttg atcacaaccg
     4621 gggcggggga ccattttaat tccggattct gcttgggcag gctccttgga ctcgacaccc
     4681 acgagagcct gctgtgcggg gtgacatgca gcggtttcta tgtccgcacc gcgcggagcc
     4741 ccggagtgaa cgatctggtc cgaatgctga aggacaagac agtcaagtac tagcacgtgt
     4801 cgcaatgcgt ttgaacgtgg tcaactatgg gcatccggcg ctgcgccagc gcggcgcacg
     4861 aatcgaagcc gtgacgcccg aaatccagtc tctggcggcg aacatgatcg agaccatgca
     4921 cgccgcacat gggatcgggt tggccggcca gcaggtggcg aagccgctcc agatcttcgt
     4981 gctcgatctg cgcgaggtca gcgaccggcc ctcgacgctc gagtgggagg gagagccagc
     5041 caatatcgcg gaccgcatgc ccatggttct catcaacccg gaagtgaccc cgctggacgg
     5101 gatcaccgat accggtcccg aggggtgcct gagcttccct gaaatcttcg ccgagaagat
     5161 cacgcgcccc gcttccgtgc gcgtcactgc ctggaacgag cgcggggaga tcatccgttt
     5221 cgcctgtggc ggcttgttgg ctcgcgccgt gcagcacgag cacgaccatc tgcgcggcat
     5281 cctgttcatc gaccgcatga catccgcgga aaggcgcagg tggcagccgg aactcgacgc
     5341 tctgcaagac gccaccagga ccgagctcca gaagggccaa atcatgcagc gggaatcgct
     5401 gggagactgg aagcgcattg tgtaatgcag tctcggtcac ttcaactcct tgcccgagcg
     5461 tattacgccg atactgtgga gtcgttcttt gctgcaagcc gcgagacagt gctgggcaca
     5521 ctggtaacga actgcgaaag ggctgttgag cctacccaac gcgatgcttg gctccatcgg
     5581 atcgattttc tgcgaacgac cctgtttggc ctcagcggca ctttgttcct cgaattcaac
     5641 attccccgaa tgggacgccg catcgacgtc gtgttcattc aaggcgcagt tgttttcgtc
     5701 atcgagttca aggttggtga gcgccacttc acgcgtgatg atatcgacca ggtctgggac
     5761 tacgccctcg atctgaagac gccgaccatt attgaagcgg cacagaggct ctatgcgcgg
     5821 cattccgtcg atgccattgc acgacatgat gccggcgcac gcaacctggc catcacctcc
     5881 aggcgaattg aggaactgat cgacgaggcg ctggcacagg atttcttcgg gatgatacac
     5941 cgctgccaaa cacgggaaga acatgtgcac tggggcagcc gggttagcgc ggcacggcaa
     6001 tcgcagtacc tcaagcggac atttcgtctc cgccctctca aactacaaac tcgttccgtc
     6061 cgggcaaatc gctcccgagc accttcgaac ctacgactcc tgagaggaga caccgtggct
     6121 caactcagaa gagatggaat cgacagtgca cggggcacgg accattcagc ccagtcgcgt
     6181 ggggaagcga atgccattcc gatactcggt gccgacctgt tcgagtccgg tcaagtcttg
     6241 tcgaccaagt gcaaccgtgg cggagcgcac ggcaatctga ccccggtgaa acaagccgtt
     6301 attctggcgg ggggcaaggg aacgcggctg cgctcggttc tgggcgatct gcccaagccc
     6361 ttgattccaa tcggcggcag accgttgctc gaacaccagt tcgagttgcg ccgccgccac
     6421 ggattcaacc gtgtcctggt gctcgccggg catcgggccg agcgcatcga ggcccattgc
     6481 ggcgacggga gccgctgggg gctgcagatc gagtgcgaga tcgagcgtga accgctgggc
     6541 acggcggggg ccgtcctggc cgcgttttca cggctgcccg agcgatttct cgtgctctac
     6601 ggggatgaga tgctcaatgt cgacctcgac aggatatggc gcgcccatat cgcggccggt
     6661 gcggcggcca ccctgttgct ccaccccaac gatcacccct ttgattcgga tctggtcgac
     6721 gtcgacgatc aggactggat tcgcgcgttt caccatcgcc cgcatccgcc ggaagctctg
     6781 tttcagaacc tggtgaacgc ggcgttgtac gtcgtcgagc gatccgccct gagcccatgg
     6841 gctggctcga acaaggctct cgatttcggt caggacctgt ttccgggtat gctcgatcgg
     6901 gggaacaggc tgctcggcta tcgaagcccc gagtacatca aggacatcgg cacgccggac
     6961 cgctacgcgc gggtctgccg ggagtacgag gccggcgtgg tggcggccag cagcctcgcc
     7021 gtgccgcaga aggcggtctt cctcgatcgc gacggcactc tcaaccggga agtgggcggc
     7081 gtttgcgcgc cggatcggct cgagttattg cccggcgtgc ccgccgccat acgcgaactc
     7141 aatcaccatg ggtggcgtgt ggtggtcgtc accaaccaac cggtggtggc gaagggcttc
     7201 tgcaccgagg ccgatatcca acgcgtccat aacaagctcg aaacgctcct cggcgccgcg
     7261 cacgccttcg tcgaccgcat ctactggtgt ccgcaccatc ccgagcgcgg ttttcccggc
     7321 gaacgcccgg atttgaagct cgactgcgcc tgtcgcaagc ccaagccggg cctggtgctg
     7381 cgggccgcgc acgatctcaa tatcgacctg cgtgcctcct ggttcgttgg cgacagcacc
     7441 acggacctcg ccacggcctc gaatgcgggc gtccgctcga tcttggtccg gaccggacac
     7501 gccggacggg acggcaagtt cgaatcccgg ccggatcatg tgttcgagac tctgaccgag
     7561 gccgttcagt gcatcgtgag cgcaggcaac gcgcgccggg tttgctcgga cacacgaggg
     7621 gcagcgcgtg aattcgaagc gaacggcatt caggattcga catcatagcc tgagctttca
     7681 ccatgatcat cagtcgcaca cccctccgga tgagcttcgt cggaggcggc agtgacctgc
     7741 cggcctacta ccgtctccac ggcggcgcgg ttgtgtccac cgccatcgac aagtacgtct
     7801 atgtgaccgt caatcccaag ttcgacaaca acatccgtgt cagttacgcg cggactgagg
     7861 aagtggacga agtggctagg ctgcggcatc ccctggtccg tgaatcgctc cgccacttgg
     7921 acatcgccgg gggtgtcgaa atcacatcga tcgccgacat cccgtcccgc gggacggggc
     7981 tcggatcgtc gagcgcgttt acggtaggcc tgcttcacgc gctccacgct tggcaggggc
     8041 gctatcgatc ggccgagcag ttggccaggg aaagctgcct gatcgagatc gagcggtgcg
     8101 gggagccgat cggcaagcag gaccagtacg cagccgcatt cggcggcttc aacttcatcg
     8161 agttctccgc cgacgaacgc gttgtggtcc agccgatcgt ctgtcggcac gagacgctcg
     8221 agcggcttca ggacaacctc gtcgccttct acacgggcat cacccgaccg gccgccaagg
     8281 tcctacgcga gaaacagcgc cggatcgcgg ccgaatcgga caagcaggcc atgctcgggc
     8341 gcatggttgc cttggcgcgc acgctgcgcg acgaactcca gaacaacaac ctcgacgcct
     8401 tcggagaggt catccaccag aactgggtgt tgaaaagaca gctctcggac gacgtcagca
     8461 ccggccagat cgatgcctgg tacgacgcag cccggggcgc aggcgcggtg ggcggcaaaa
     8521 tcctgggggc cggttccggc ggcttccttc tgttctacgc gccgcggcgg cggcaccggg
     8581 cgattcgcga tgccttgagc gagttgcgcc ccatcccgat gcgtttcgag cctcagggaa
     8641 gccggatcat cttcgtgcac tgaacccgaa aaccggaaac gccggcggct cagcgccaat
     8701 tcatgaagt
//
LOCUS       DAOQRO010000125         8604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7689_length_8604_cov_5.582290, whole genome shotgun sequence.
ACCESSION   DAOQRO010000125 DAOQRO010000000
VERSION     DAOQRO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_8604_cov_5.582290"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKK20_06285"
     CDS             complement(<1..654)
                     /locus_tag="PKK20_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99527.1"
                     /translation="MRARNANASNESRPGQSAVPSSSGGRINLRFFRQTGWMVVATTL
                     GGGFMYLVHIPAAAGMPRAEYGVFQALLQVMNLMLIPAIGLQTIIAQQTASALTDLQH
                     RQLAATVRVLLAGVGVIWLVMALSTWVMRDRLLAALQIAHPAALWVTVLIGLAMLAWP
                     ILQGVLQGRQSFFWLGSLQVVNGGGRLLGVLILVWFLGGQAAGAMTAALLGFWATVTL
                     "
     gene            complement(749..1999)
                     /locus_tag="PKK20_06290"
     CDS             complement(749..1999)
                     /locus_tag="PKK20_06290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU99528.1"
                     /translation="MTLAGTSATRHRMRLALLTHEPLHPPTGGGSAEALYLVREFVRR
                     GHDVHVFCPTVPEADRLERDLGVRLHPFTLWRMGRYASLRSLKYLVYPLFLERLVARV
                     AREIRFDVLFSQHAISAVAAGRLKRRLGAALVMNQLDYLTGFMETWPAWLMPPPLLAL
                     LMRYELSLPRRFGADRVLTVSDALADRVAAAGFPRERLESIYYGYDSALFPFDAAAVA
                     ARSDDPPVVVMHGSFDRHHLGPIALEAVAHVRAERPDTVFRFVGQPTDTLRSFLEAAR
                     KRGAGGGVECPGFVPYREMARHLAGASVGIVPYEASAGVHCAFVAKVVEYLALGLPVV
                     CTALEGIQRYFPDEPLIRYARFQGRHFGEQILAWLRTPLAERRKMSQPASLRVACKLD
                     WSVICRRAADAVEDASRSQPRANP"
     gene            complement(2019..3098)
                     /locus_tag="PKK20_06295"
     CDS             complement(2019..3098)
                     /locus_tag="PKK20_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNU99529.1"
                     /translation="MNPAFLPAGHPWAGEQRVCDFLAATAARLGLDLDFQVVESGRRN
                     LLARLCPVGHVRHRVILAPHLDTVGASDNQLTPRAARGRLYGRGACDTKGSIAAMLTA
                     LSRVAGSPRRPAATEIVFAGLIDEEHGQIGSRFLARSGLEGDLAIVGEPTQLEVVTAH
                     KGNVWMQLETRGKAAHGAHPDLGVNAVHDMARIVHLLETKHSSRLRRHAHPLLGCGTI
                     NIGVIAGGTQPNIVPDRCAIQIDRRTLPGENEMAVIGQVRSLLRQNRLRARVTSFKEA
                     ACPPLETNPRLPFVSRFLEAARQTRPAGVAFFCDAAVLAAAGIPSVVFGPGDIAQAHT
                     EDEWISLRALQRATDLLTRFLLSLP"
     gene            complement(3055..3492)
                     /locus_tag="PKK20_06300"
     CDS             complement(3055..3492)
                     /locus_tag="PKK20_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99530.1"
                     /translation="MAELSAIAIEKARLYERLGEVENQLRKNEKLSVLGLLAAEVAHE
                     IRNPPAAMQMLYHSLDLRFPDGDPRARDARTAGPVPSPGDRTPTTTRCSGLRGATHGS
                     LAHDAAVRQTGGNHHGCCRSIARFDCAAERESGLSACRPPLGG"
     gene            complement(3532..4734)
                     /locus_tag="PKK20_06305"
     CDS             complement(3532..4734)
                     /locus_tag="PKK20_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNU99531.1"
                     /translation="MKRPCRRLRCGASALDFPMRGPDNAGRMRLLDRYLLRELLVPLG
                     YCLGGFLIFWITSDLISDLDVFQRFQLLPRDVAAYYLVRMPELLVTVLPVALLLALLY
                     TLTNMARHQELTAMRAAGLSLWRLCVPYLVVGSILGGVLFALNEVWVPDAYTASRLIL
                     RRRIPVAPGDLGPEWQRNLVFHNDANSRLWQIGAFNRLTGEMLSLNLEWRFPDGGHRR
                     LAAERADYTNGHWVLQDVKELLYTPESRLPAFRGMTNRLALAELTETPELINSEIRFT
                     ELSNVKAAKGARLSLREILDYERLHPRLNPRARAKLETQFHGRLAEPWKCIVVVLIAI
                     PFGAPSGRRNVFVGVASSIFIAFAYFFIAGFSLALGTGDYIAPWLAAWLPNALFTGAA
                     IWLMQRIQ"
     gene            4999..6852
                     /locus_tag="PKK20_06310"
     CDS             4999..6852
                     /locus_tag="PKK20_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99532.1"
                     /translation="MNNILTVLRFGCGYLPRYWVRLALSVVFGGVFALTNGSFMWATR
                     TLTERFAPAAAAPAQPGPSDARDRFSVAIERLGQSVQRAIDPWLPRAGQPLEWRQAVG
                     LLLLLPILVFLRSGADYGNNYCLGWVSERVVRDMRLDVMEKLSQLSLEFFTRSRTGDL
                     LTRINVDTYNLLRALRQGVADLIKESFTIMVVFCGLLWLDWRLTCAAMVLLPACLLPL
                     MVLGRKAHRAMAVSRKANVLQSSQLVELLGSIRVVKAYNLEAVEMARFRRTSGQLVRA
                     GMKGVKAKELVNPLIEIISVLGLGALLLYVFQAGRSGPELVAFITGILLFFLPLKKLA
                     GVHIVFEQASVGVHRLSELLAEQPKVVEAPNPKPVSGFESELRFERVSFAYDDRVVLK
                     DVNLVIPRGARLGVAGDSGSGKTTLLNLLFRFYDPTSGMIRIDGTDLREVSIRDLRGQ
                     LALVSQDVTIFDQTVAENIACGRSGASIEEVEAAARAASAHEFILALPEGYDTRVGER
                     GVTLSGGQRQRVAIARAFVRNAPILVLDEATAALDSKAEAEVQAAIDRLAEHRTVICV
                     AHRLSTLAGMDQIIVLSRGCIVETGGFKALLNRGGVFAAMAARQGLYPSSG"
     gene            complement(7105..7242)
                     /locus_tag="PKK20_06315"
     CDS             complement(7105..7242)
                     /locus_tag="PKK20_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99533.1"
                     /translation="MADSLRPDRTARARLDSRRPGTLNLGPMLARDHRGVRHPLKLDH
                     A"
     gene            7241..>8604
                     /locus_tag="PKK20_06320"
     CDS             7241..>8604
                     /locus_tag="PKK20_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNU99534.1"
                     /translation="MIVLGMGGMVGHDPAAALVVDGRVVAAAEEERFLREKHAKGKQA
                     ERAAAFCLRFAGLKASDVQVVAIPFAPVSLMSPARWHYAVRHWYAPDRAFTALFNGNR
                     RFRRNARQVFAALERMGIDRRRVRFVPVEHHLAHASSAYHVSGYADAAIMSVDGVGEY
                     CTTWFGHGSGGEIACLKEFYAPDSLGGCYGAVTEYLGFEMLDGEFKVMGMAPYGDPDR
                     ADVSGLIEWSERGFRVNTRLVNCIGLRRYKRAAPVEAQGIGTYFSPKLVKLWGPPRVG
                     DEIDHPYVHIAAAVQRQLEQILLALTRHYLGGHIARTRRLCYAGGVALNVKANQRLIQ
                     AFAPDCEVFVQPAASDAGTALGAATYAAHRLGDTIQPMTHAYLGPEYSAAEIEEALCK
                     RGCRYERCASITDRASDLLAKGQVVAWCQGRMEWGPRALGNRSILGNPAVPGVADTIN
                     AQIK"
BASE COUNT         1484 a         2661 c         2975 g         1484 t
ORIGIN      
        1 cagcgtgacg gtggcccaaa agccgagcag tgcggctgtc atggccccgg ccgcctggcc
       61 tccgagaaac cagacgagga tcaacacgcc caggagtcgt ccgccgccgt tcaccacctg
      121 gaggctgccg agccagaaga agctctgtct gccttgcaac acgccttgga ggattggcca
      181 cgccagcatc gccagcccga tcaagaccgt cacccacaag gcggccggat gggcaatctg
      241 cagcgccgcc agcagcctgt cccgcatcac ccaggtggac agcgccatga ccagccagat
      301 gacgcccacc ccggcgagca gcacccgcac cgtcgccgca agctggcgat gctgcaaatc
      361 ggtcagggcc gacgcggtct gttgcgcgat gattgtctgg aggccaatcg ccgggatcag
      421 catcaggttc atgacctgaa gcagcgcctg gaaaacgccg tactccgctc tgggcatgcc
      481 ggccgccgcc gggatgtgca ccaggtacat gaatccgccg cccagcgtcg tggccaccac
      541 catccaaccc gtctgccgaa agaaacgcag gttgatacgc ccgccgctcg aagacgggac
      601 cgcggactgt ccgggacgcg attcgttcga ggcgttggcg tttctagccc gcatgtttca
      661 cgtcccgatg gtgcacgggg cgctcagact tcgcaagagg actgctatgc gggctggaaa
      721 agcatgtttc atgaccgggt cttttctctc acgggttggc ccgcggttgt gaccggcttg
      781 cgtcctcgac cgcatcggct gcgcgccggc aaatgacgct ccagtcgagt ttgcacgcca
      841 cccgcaggct cgccggctgt gacatcttcc ggcgctcagc cagcggcgtg cgcagccagg
      901 ccagaatctg ctccccgaaa tggcggcctt ggaatcgcgc gtaacggatc agcggttcat
      961 ccggaaagta ccgttggatt ccttccaacg ccgtgcagac gacgggcagg cccagagcca
     1021 ggtactcgac caccttcgcg acaaatgcgc aatgcacacc cgccgacgcc tcgtacggca
     1081 caatcccaac cgacgccccc gccagatgcc gcgccatctc gcgatacggg acgaaccccg
     1141 ggcattccac gccgccgccc gcaccgcgtt tgcgcgccgc ttcgaggaag ctgcgcaatg
     1201 tgtccgtggg ctgtccgacg aatcggaaca cggtgtcggg gcgctctgcc cggacatggg
     1261 cgacggcttc gagggcgatg ggcccgagat gatgacgatc gaaggatcca tgcatgacga
     1321 ccaccggcgg gtcatcgctg cgagccgcca cggcggcggc atcgaagggg aacagggccg
     1381 aatcgtatcc gtagtagatc gattcgaggc gttcccgagg aaagccggcg gcggccacgc
     1441 gatccgccaa ggcatcggac acggtcaaga ccctatcggc tccgaaccgc cggggcagcg
     1501 acagttcgta gcgcatgagc aaagcgagca gtggaggggg catcagccag gccggccatg
     1561 tctccatgaa accggtgagg taatcgagct ggttcatgac cagcgccgct ccgagccggc
     1621 gtttcagccg tccggcagcg acggcggaaa tggcatgctg cgaaaacagg acatcgaacc
     1681 ggatttcccg tgcgactcgc gcgacgagtc gttcgagaaa cagggggtac acgaggtact
     1741 tcaagcttcg cagcgaggcg taccggccca tgcgccagag tgtgaacgga tgcaggcgca
     1801 ctccaaggtc ccgctcgagg cggtccgcct cgggcacggt cgggcagaac acatggacgt
     1861 cgtgtccgcg tcggacgaat tcgcgcacca gatagagcgc ctccgccgaa ccgccgccgg
     1921 ttggcggatg caagggttcg tgcgtcagga gcgcgagccg catccgatgc cgcgtcgccg
     1981 atgtgccggc cagggtcatt gctttctggc ctgcgccttc acgggagcga caataggaac
     2041 cgcgtcagaa gatccgtcgc ccgttggagc gcacgcaacg agatccactc gtcttccgtg
     2101 tgcgcctgcg cgatatcccc ggggccgaag acgacactcg gaatgcccgc ggcggccagg
     2161 accgcggcgt cgcaaaagaa cgctacgccc gcgggccggg tttgtcgggc ggcttcgagg
     2221 aagcggctga caaacggcaa tcgcgggttg gtctcgagcg gcggacaggc cgcttccttg
     2281 aagctggtca cccgtgcccg aaggcggttc tgccggagca gagaccggac ttgtccgatg
     2341 acggccatct cattttcgcc tggcagcgtg cggcgatcga tctggatggc gcagcggtcc
     2401 ggaacgatgt tcggctgggt gccgccggcg atgacgccga tattgatcgt cccgcagccg
     2461 aggagcgggt gggcgtgtcg gcgcaaccgg ctcgagtgct tggtttcgag cagatggacg
     2521 atccgggcca tgtcatggac cgcgttgacg cccagatcgg ggtgcgcgcc gtgcgcggct
     2581 tttccacgcg tttcgagctg catccagacg ttgcccttat gggcagtcac gacctcgagt
     2641 tgggtcggtt cccccacgat ggccaaatcc ccctcgagac ccgagcgcgc caggaacctg
     2701 gatccgatct ggccatgttc ttcgtcgatc aggccggcga agacgatttc ggttgcggcg
     2761 ggtcggcgcg gcgaccccgc cactcggctc agcgcggtga gcatggcggc gatgctgcct
     2821 ttggtgtcgc aggcgccccg gccatagagc cgacctcggg cggcgcgtgg tgtgagctgg
     2881 ttgtcggatg cgcccacggt atcgaggtgc ggcgccagga tcacccgatg acggacgtgc
     2941 ccgacggggc agaggcgggc caggaggttg cggcggcccg attcaacgac ctggaaatcg
     3001 aggtcgaggc ccagtcgggc ggcggtggca gccaggaaat cgcacacgcg ctgttcaccc
     3061 gcccaagggt ggcctgcagg cagaaaggcc ggattcacgc tcggcagcgc aatcaaatct
     3121 cgcaatagag cggcagcatc catgatgatt tcctccagtc tgccggaccg cggcatcgtg
     3181 agcaagcgag ccgtgcgttg caccccgcag tccggagcat cgggttgttg tcggtgttct
     3241 gtcgcccggt gagggcaccg ggcctgcagt ccgggcgtcg cgcgcgcgcg gatcgccgtc
     3301 gggaaagcgc aggtcgagcg aatgatagag catctgcatc gcggccggcg gattgcgaat
     3361 ctcgtgggcg acctcggcgg ccaggaggcc gagcacggag agtttctcat tcttgcgcag
     3421 ttggttttcg acctcgccca ggcgctcgta gagacgcgcc ttctcgatgg cgatggccga
     3481 gagttcggcc aaggcggaca ggctgcggat ttcttcgttc gagattgctg ttcattggat
     3541 gcgctgcatc agccagatgg cggcgcccgt gaacagggca ttggggagcc aggccgccag
     3601 ccagggggcg atgtagtcgc ccgtgcccag ggcgaggctg aagccggcga tgaagaaata
     3661 ggcgaacgcg atgaagatgc tgctggctac tcccacaaaa acgttgcgcc ggcctgacgg
     3721 ggcgccgaac ggaatggcga tcaggacgac cacaatgcac ttccacggtt cggccagccg
     3781 gccatggaat tgggtctcga gcttggctcg agccctcggg ttgaggcgcg ggtggagccg
     3841 ctcgtaatcg agaatctcgc gcagcgacaa ccgcgctccc ttggccgcct tcacgttgct
     3901 cagttcggtg aaccgaatct cgctgttgat gagttcgggc gtctcggtca gctcggccaa
     3961 ggccagccga ttggtcatcc cgcggaaggc cggcagccgg gactcgggcg tgtagagcag
     4021 ttccttgacg tcctgcaaga cccagtgccc gttggtgtag tccgcccgct ccgccgccag
     4081 cctccgatgc cccccgtcgg ggaagcgcca ttcgaggttg agggacagca tctcgccggt
     4141 gaggcggttg aaggcgccga tctgccacag acggctgttg gcgtcgttgt ggaagacgag
     4201 gttgcgctgc cactcgggtc cgagatcgcc cggtgccacc gggatgcggc gccggagaat
     4261 cagccggctc gcggtgtagg cgtccggcac ccacacctcg ttgagggcga agagcacgcc
     4321 gcccaggatg gagccgacca ccaggtaagg cacgcaaagc cgccacagac tcaggccggc
     4381 ggcgcgcatg gcggtgagtt cctgatggcg cgccatgttt gtgagggtgt agagcagggc
     4441 cagaagcaag gccaccggga ggaccgtgac cagcaactcg ggcattcgga ccaggtaata
     4501 ggcggccacg tcgcgcggga gcagttgaaa gcgctggaaa acatccaggt cgctgatcag
     4561 atcggaagtg atccagaaga tcagaaagcc cccgagacag tagccgaggg ggaccagcag
     4621 ttcgcggagc agataacggt cgagcagccg catgcggccg gcattgtccg gaccgcgcat
     4681 gggaaagtcg agcgccgatg cgccgcatcg cagtcggcgg cacggtcttt tcatttggag
     4741 cggtgcttga cgagtatctg aaaagggatt tggggattgt tctggcagag aaagtgacag
     4801 gttcttaatt tgacagtggt ttgtcgagat catcgcacgc ctactgcgta ccaaaccatg
     4861 ccaattgaca ggcgctattt ccaacctcaa caacagcaac ccttcaggaa tttaaaatga
     4921 aataaccgtg cagtcggcga gccgggcgcg cgatttggta tggccaaagg ccgttcgact
     4981 ggcgaacatg ggttgggcat gaacaacatt cttaccgtcc tgcgctttgg ctgcggctac
     5041 ctgccgcggt actgggtgcg gctggcgctg agcgtggtgt ttggcggggt gtttgcgctc
     5101 accaacggca gtttcatgtg ggcgacgcgc acgctcaccg agcggttcgc gcccgccgcg
     5161 gccgcgccgg cgcagccggg gccttcggac gcccgggacc gcttctccgt ggcgatcgag
     5221 cgtttggggc agagtgtgca gcgcgcgatc gatccctggc tgccgcgcgc gggccagccg
     5281 ctggagtggc ggcaagcggt gggcctgctc ctgctcctgc cgatcctggt cttcctgcgc
     5341 agcggcgccg attacggcaa caactactgc ctcgggtggg tgagcgagcg cgtcgtccgg
     5401 gacatgcgac tggacgtcat ggagaagctg agccaactct ctctcgagtt ctttacccgc
     5461 tcgcggacag gcgatctgct cacgcggatc aacgtcgaca cgtacaatct gttgcgggcc
     5521 ttgcggcagg gagtggccga tctcatcaag gaatcgttca cgatcatggt ggtcttttgc
     5581 ggcctgctgt ggctcgactg gcgattgacc tgcgcggcta tggtgctgtt gccggcctgc
     5641 ctgctgccgc tcatggtgct gggtcggaaa gcacaccgcg ccatggccgt gagccgcaag
     5701 gcgaacgttt tgcaatcgag ccagttggtc gagttgctgg gcagcatccg cgtcgtcaag
     5761 gcctacaacc tcgaagccgt cgagatggcg cgtttccgtc ggacttccgg gcagctcgtt
     5821 cgcgccggca tgaaaggcgt caaggcgaag gagttggtga atcctctgat cgagatcatt
     5881 tcggtcctgg gtttgggggc gctgctgctt tacgtctttc aggcgggccg cagcggcccg
     5941 gagctggtgg ccttcatcac cgggattcta ctctttttcc tgccgttgaa gaagctggcc
     6001 ggcgtgcaca tcgtcttcga acaggccagt gtcggcgtcc accgcctgtc ggaactcctc
     6061 gccgaacaac ccaaggtcgt cgaggcgccc aaccccaagc ctgtgtccgg gtttgaatcc
     6121 gaactgcgct tcgagcgcgt gagcttcgct tacgacgatc gagtggtgct gaaggatgtg
     6181 aacctggtca tcccccgcgg cgccaggctc ggtgtcgcgg gcgatagcgg gtcgggcaag
     6241 accacgctgc tcaacctgct gttccgcttc tacgacccga ccagcgggat gatccgaatc
     6301 gacggcacgg atttgagaga ggtgtcgatc cgcgatctcc gcggtcaatt ggcgctcgtg
     6361 agccaggacg tgacgatatt cgaccagacc gtggcggaga acatcgcctg cggacgttcg
     6421 ggcgcgagca tcgaggaggt cgaagccgcg gcgcgcgcgg ccagcgccca cgagttcatc
     6481 ctggctctgc ccgagggtta tgacacgcgg gtcggcgagc ggggcgtgac gctctccggc
     6541 ggacagcggc aacgggtggc catcgcgcgc gcgttcgtgc gcaacgcgcc gatcctggtg
     6601 ctcgacgaag ccacggctgc cctcgactcg aaggccgagg ccgaggtaca ggcggccatc
     6661 gaccggctgg ccgagcaccg gacggtgatc tgcgtggcgc accgcctttc cactctggcg
     6721 ggcatggatc agatcatcgt gttgagccgc ggatgtatcg tggagacggg cggcttcaag
     6781 gccctgttga atcgaggcgg agtcttcgcc gccatggcgg cgcggcaggg cctctatccg
     6841 agctcgggtt gacgcctgtg cggcggcgcg ggactgcgcc ccttcgcgcg tgggtggcga
     6901 ggttccgagg cgccccggag cgccggcttg cagcccgctt aacgcggggc cgcacctgga
     6961 gagtttgctg tccggtgggg agtgcaatgg gcaacagttg gcttccaaca cgaggcgctg
     7021 tgcgtatcgt tcacggggct tgaagatcgc cgtgttgaag atcaccgttg ccgcctcttg
     7081 cggcgatggt aagctcgcgg cctttcatgc atgatcgagc ttcaacggat gccggacgcc
     7141 tcgatgatcg cgggcgagca tcgggccgag attcagtgtg cccggccggc gggaatcgag
     7201 ccgggctcgg gcggttcgat cgggacggag ggagtcggcc atgatcgtgc tcggtatggg
     7261 aggaatggtc gggcacgacc ccgctgcggc gctggtggtg gacgggcgtg tggttgcggc
     7321 ggcggaggag gagcgtttcc tgcgggagaa gcacgcgaag ggcaagcagg ccgagcgtgc
     7381 ggcggcattc tgtctgcggt ttgcggggtt gaaagcgtcg gatgtgcagg tggtggccat
     7441 tccgttcgcc cctgtcagcc tcatgagccc cgcccgatgg cactatgcgg tccgacactg
     7501 gtacgcgccg gatcgggcgt tcacggcgct gttcaacggc aaccggcgtt ttcggcgcaa
     7561 tgcgcgtcag gtcttcgccg ccctcgaacg tatgggcatt gaccggcgtc gcgtccggtt
     7621 cgtgcctgtc gagcatcatt tggcgcacgc ctcgagcgcg tatcatgttt ccggatacgc
     7681 cgacgccgcc attatgagtg tggacggggt gggcgagtac tgcacgacgt ggttcgggca
     7741 cggatcgggc ggtgagatcg cctgtctcaa agagttttat gcgcccgact cgctgggcgg
     7801 gtgctatggc gccgtgacgg agtatctcgg cttcgaaatg ctcgatggcg agttcaaggt
     7861 gatgggcatg gcgccctacg gcgatccaga ccgggcggat gtgtccgggc tgatcgagtg
     7921 gagcgagcgg ggcttccggg tcaacacgcg gctggtcaat tgcattggac tgcgccgcta
     7981 caaacgcgcg gccccggtcg aggcgcaggg catcggaacc tacttctcgc ccaagctggt
     8041 caagctgtgg gggccgccgc gtgtcgggga tgagattgac catccttacg tgcacattgc
     8101 cgcggcggtc cagcgccagc tcgagcagat tctcctggcc ctgacgcggc attatctggg
     8161 cgggcacatc gcgcgcacgc gccgcctctg ttacgccggc ggcgtggcgc tcaacgtgaa
     8221 agccaatcag cgcctcattc aggccttcgc gcccgactgc gaggtctttg tgcagcccgc
     8281 ggccagcgac gcgggcacgg ctctcggcgc ggccacgtat gccgcccatc ggcttggcga
     8341 cacgattcag cccatgacac acgcgtatct cgggccggag tattcggcgg ccgagatcga
     8401 ggaggcgctg tgcaaacgcg gctgccgcta tgagaggtgc gcgtcgatca ccgacagggc
     8461 gagcgacctg ctcgccaaag gccaggtggt ggcgtggtgc cagggccgca tggagtgggg
     8521 tccgcgcgcc ctcggaaatc gcagcatcct gggcaacccc gcggttcccg gcgttgcgga
     8581 caccatcaac gcgcagatca agta
//
LOCUS       DAOQRO010000126         8547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7794_length_8547_cov_5.595384, whole genome shotgun sequence.
ACCESSION   DAOQRO010000126 DAOQRO010000000
VERSION     DAOQRO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_8547_cov_5.595384"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="PKK20_06325"
     CDS             complement(<1..1902)
                     /locus_tag="PKK20_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU99535.1"
                     /translation="MLSLSEVSKAYAGRTLFADVSLQVNREDRIGLVGPNGAGKSTLL
                     GLILGTTAPDTGTIALQRGITVGYLPQENAPVGDETVLELASAITPEIGRLRRQLRAF
                     EAEHAADTPEYHDAQARFDQLGGSTLEAKAKKVLAGLSFRPADFERPASALSGGWVMR
                     AYLARLLTLGPDLLLLDEPTNHLDLEALLWFQEYLRDYPGAMVLISHDREFLNQLTTS
                     ILEIRQGRLFRYRGNYDAYLEQRAANEAQLLAAWKHQQREIARLQAFVDRFRAKNTKA
                     AQAQSKLKQIERMVKLEAPSGAEATVAFAFPQPQRAGRRVVALEGVRFAYGEQLIYDK
                     LDFEAERGRRIVLVGPNGAGKSTLLKLLAGVLVPQAGERRLGHNVSAGYYSQYRVDTL
                     HLDRTVLEEAFDTPQALTETFVRTVLGSFLFRGDDVFKRVGVLSGGEKSRLALVKLLL
                     DPPNLLLMDEPTTHLDLASVEALLSALQQFDGTIVFISHDVYFIRQLANHVVHIHNGR
                     LTAYPGDYQYYVDKTAALRALESGGADSAEGRNPSTKQTATSVAGRRREQKRLAAQER
                     QARYRELQSVRQRVRQLEHDIAAGEARQAELTAALEDPETYADSSRAVALNREITEVI
                     GDLERLTAEW"
     gene            complement(2069..3865)
                     /locus_tag="PKK20_06330"
     CDS             complement(2069..3865)
                     /locus_tag="PKK20_06330"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNU99536.1"
                     /translation="MKTTLLALGFALALDALAAVDLELARDEKAACVIVRQAGATLPE
                     QNAIRELSETLEAVTGAQFSVQEAGDQAPERAIVVGCGSLAAKLFPEVPFDQLGEEEI
                     VIKRKGNCLLLAGGRPRGTLYAVSQFLQDQCGVRWWTPWASQMPKHAVLAVGALDVRY
                     RPTFESRDPFWYPAFNARWAVRNFSNSQSANIGADWGGCIRYKGFVHTFYPLVPPETH
                     FAAHPEWYSFRDGKRSVDHGQLCLTNPDLRDHVVARVKEWLRESPDARIVSVSQNDWH
                     GACQCEACKALDEAEGSHAGTLLDFVNHVAAGIEAEFPQVAVDTLAYQYTRKPPKTIK
                     PRANVIVRLCSIECNFREPLEAPANATFADDIRGWEKICDRLYIWDYTTDFAHYVQPH
                     PNWFVLGPNVRFFAAHNVRGLFEQGAYQSYGSEFSELRSWVLAQLLWNPQKDDRGLID
                     AFLDGYYGKDAAPFIRTYIDLMHGASTGWNLTCFSRTDTPFHNFKTLSEADRLWRQAE
                     TASAGAPDLLRRVQRGRMWLGYVWLNLWDKLRKECADAGADWPMPASRKVYAAEWLKL
                     AQGDPDHPWTKVTHINESGVTPQKFVERFAND"
     gene            3977..6703
                     /locus_tag="PKK20_06335"
     CDS             3977..6703
                     /locus_tag="PKK20_06335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU99537.1"
                     /translation="MPDAIQTPSDLCLTPHGLLLVMDGPPPSPWADAETGCRVAQAFA
                     EAPARGLLHLATRELNAALPPSAAWWRELARRHLTRVCHIPDLDQMQQITPIPPPAPD
                     ELDALADTAPPMRGGEYLNAEMLASLWNGLDALVRAEASRHPRGAGAWLKESHPLWRM
                     VGRVTFHLAENKRHPTHPFAFMASYASRISAQSRVQHLPLGRALQEYAGAGNKPALVN
                     LLSPVQRAAEKSRFVRELVETGRIFKALPWTPAEAYRFLQDTPVCEQAGVLVRLPDWW
                     KGGRPPRPRVSVRVGQNRGAGLGVDTLLDFSVQTTLDGEPLTDAEWQSLLEAAEGLAL
                     VRGKWVEVDPAKLRAALDHWKKVEALARRDGLSFFEGMRMIAGFRPGADDAAQTPAYE
                     AEREWSGIQPGQWLDTVLAELRDPSRLGAASNIPGLRAQLRPYQALGVHWLEFLAQLG
                     LGGCLADDMGLGKTIQVLALVLKRKTEARPPHRPNLLVVPASLLANWRAEIERFTPTL
                     SFAIIHPSEAHDPAAALESASDGGTDLVITTYGMLARLESLRRRAWNLAILDEAQAVK
                     NAGTRQARAVKELQAAARFALTGTPVENRAADLWSIFDFLNPGLLGSAQAFSRFVRAR
                     GDSPQPDYGPLRALTRPYILRRLKTDKRIIADLPDKTEVRAWCALTPAQAALYQKAVT
                     ELAEGLEAADGIQRRGLVLAFIMRFKQICNHPSQWSGDGGYAPEASGKFQRLRELVEE
                     IAERQEKALVFTQFREITDPLAAFLAELFGRPGLVLHGGTPVAQRRKLVESFHRDDGP
                     PFFLLTVKAGGAGLNLTQAAHVIHFDRWWNPAVENQATDRAFRIGQKRNVLVHKFVCR
                     GTIEERIDRLIESKQSLSRELLEGGGETLLTEMSNEELLRFVALDLNRATEA"
     gene            6718..7590
                     /locus_tag="PKK20_06340"
     CDS             6718..7590
                     /locus_tag="PKK20_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNU99538.1"
                     /translation="MSWYSYGFRPYVSVAERQRKAQREMDRLAKQGKVIHPIRLEGRT
                     IARTFWGKAWCDNLESYMDYANRLPRGRAYVRNGSVVHLEIGPGEIQAMVSGSSLYKV
                     RIAIAPAAKPKWKKLCQECAGSIGSLVELLQGRFSDHVMGIFTRKDTGLFPTPAEIKL
                     NCSCPDWATMCKHVAAVLYGVGARLDQQPDLLFTLRSVNQEDLVTQAATAADLTGKTP
                     SAGPELAESDLGAVFGIELDSQTAAVPATPAPDAPRAKEPRRSRKAATAKARRPAKAK
                     RRTVKRTSRSAKPS"
     gene            7597..8217
                     /locus_tag="PKK20_06345"
     CDS             7597..8217
                     /locus_tag="PKK20_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99539.1"
                     /translation="MQDSSNSIYNLEDFVPSLREALADQSLDLDDSMLLAAVPTADGN
                     PDDPPQPVELDRRLIHLVVGDNLCEWDVDSLRSLFRGDRKPPTLGDYPDAYNEEFLIL
                     EIHVLQISDIFGDRRDDEMREIYSLLRRRPDGKSIGFVHNYMWQAAAIMLGTRPLSQA
                     EFEAILSRLERSCRTFSMGPGTRNYINALRGIFEHEDSQPSKTAPR"
BASE COUNT         1523 a         2904 c         2669 g         1451 t
ORIGIN      
        1 ccattccgcg gtcagccgtt cgagatcgcc gatgacttcg gtgatttcgc ggttcagagc
       61 cacggcgcga ctcgaatcgg cgtaggtctc ggggtcttcg agagcggcgg tcagttcggc
      121 ctgccgggct tcgccggcgg cgatgtcgtg ttccagttgg cggacgcgct ggcgcacgga
      181 ctgcagttca cggtagcggg cctggcgctc ctgggctgcc agccgtttct gctcgcgccg
      241 ccggccggcg acactcgttg ccgtctgttt ggttgacggg ttccggccct ccgcggaatc
      301 ggcgccgccc gattcaagtg cgcgcaacgc ggcggtctta tcgacgtagt actggtagtc
      361 gccggggtag gcggtcaggc gtccgttgtg gatgtgaacg acgtggttgg cgagctggcg
      421 gatgaagtag acgtcgtggc tgataaagac gatcgtgccg tcgaactgct ggagggcgga
      481 gagcagggct tcgacgctgg ccaggtccag gtgagttgtc ggctcgtcca tgaggagcag
      541 attgggcggg tcgagcagga gcttgaccag cgccagccga cttttctccc ccccgctgag
      601 cacgcccacc cgcttgaaca cgtcgtcgcc acggaacaga aagctcccca gcaccgtccg
      661 tacgaacgtc tcggtcagcg cctggggcgt gtcaaacgcc tcttcgagca cggtgcgatc
      721 cagatgcaac gtatccacgc ggtactgcga gtaatagccg gcgctgacgt tgtgcccgag
      781 gcggcgttcg cccgcctggg ggacgagcac gccggccagc agcttgagca gggtcgactt
      841 gcccgcgccg ttgggtccca ccagcacgat gcgccgtccg cgttcggcct cgaagtcgag
      901 tttgtcatat atgagttgct ccccgtaggc gaaccgcacg ccttcgagcg ccaccacgcg
      961 ccggccggcg cgctgcggct gggggaaggc gaaagcgacc gtcgcctccg cgccggacgg
     1021 ggcttcgagc ttcaccatgc gctcgatctg cttgagtttg ctctgggctt gggcggcctt
     1081 ggtgtttttg gcgcggaaac ggtcgacaaa cgcctgcaag cgtgcgatct cgcgctgctg
     1141 gtgtttccag gcggcaagca gttgggcctc gttggccgcc cgctgctcga ggtaggcatc
     1201 gtaattgccc cgataccgga agaggcgtcc ctggcgaatt tcgagaatcg aggtcgtgag
     1261 ttggttgagg aactcccggt catgcgagat caggaccatg gcgcccgggt agtcgcgcaa
     1321 atactcctgg aaccagagca gtgcctcgag atcgagatga ttggtcggct catcgagcag
     1381 gagcagatcg ggaccgagcg tgagcaggcg cgccaggtag gcccgcatca cccagccgcc
     1441 gctcagcgcg ctggcgggac gctcgaaatc ggcgggacga aagctcaggc cggccaggac
     1501 tttcttggcc tttgcttcga gcgtggagcc gcccaactga tcgaaacgcg cctgcgcgtc
     1561 gtgatactcg ggtgtgtccg ccgcgtgctc cgcctcgaag gcgcgcagtt ggcgccgcaa
     1621 ccgtccgatc tccggggtga tggccgacgc cagttcgagg actgtctcgt caccgacggg
     1681 ggcgttctcc tggggcagat aaccgacggt gatcccccgc tggagcgcga tcgtgcccgt
     1741 gtcgggtgcc gtcgtgccca gaatcaaccc cagaagggtc gacttgccgg cgccgttggg
     1801 gccgaccaga ccgatgcggt cctcgcggtt aacttgcagg gagacgtccg caaacagagt
     1861 gcggccggcg taggctttgc tgacttcgct gagcgaaagc atgaatggga catcgtgtgc
     1921 gatccggggc caccgtcgca agcgcgatgc tcgcccggat gtttcacaaa gcatcgcgga
     1981 gtctgacgtc gaggctccgg caaagccgcg cccatacaag gcagaatcgc aaccacgctc
     2041 agcgaatctg gactgaatga gtcaacgctc aatcgttcgc gaaacgttcg acgaacttct
     2101 ggggcgtcac gccggactca ttgatgtggg tcactttggt ccagggatgg tcgggatcgc
     2161 cctgggccag cttgagccac tcggcggcat agactttgcg cgacgccggc atgggccagt
     2221 ccgccccggc gtcggcgcat tccttgcgca gcttgtccca gaggttgagc cagacataac
     2281 cgagccacat ccggccgcgc tgcacgcgtc ggagcaggtc gggcgcgccg gccgaggcgg
     2341 tctcggcctg gcgccagaga cggtcggcct cgctcaaggt cttgaagttg tggaacggcg
     2401 tgtcggttct cgaaaaacag gtgagattcc aacccgtcga cgccccgtgc atcagatcga
     2461 tgtaggtgcg gatgaacggc gcggcgtcct tcccatagta cccgtcgaga aaggcgtcga
     2521 tcagaccgcg gtcgtccttc tgcggattcc agagcaactg cgccaacacc cagcttcgca
     2581 gctcgctgaa ctcggatccg taactctggt aggccccctg ttcgaagagg ccgcgcacgt
     2641 tgtgcgccgc gaaaaagcgc acattcggtc cgagcacgaa ccagttcgga tgcggctgaa
     2701 cgtagtgggc gaagtcggtg gtgtaatccc agatgtaaag ccggtcgcaa atcttctccc
     2761 agccgcgaat gtcgtcggcg aacgtcgcgt tggccggcgc ttcgagcggc tcgcggaaat
     2821 tgcactcgat cgagcagagc cgcacgatga cattcgcgcg cggcttgatc gtcttgggcg
     2881 gtttgcgcgt gtactggtag gcgagcgtgt ccacggcaac ctgcggaaac tcggcctcga
     2941 tcccggccgc cacatggttc acaaagtcca gcagcgtccc ggcatggctc ccctccgctt
     3001 cgtccagagc cttgcacgcc tcacactggc aggcgccgtg ccagtcgttc tgcgacaccg
     3061 acacgatccg cgcgtcgggc gactcgcgca gccactcctt gacccgcgcg acgacgtgat
     3121 cccgcaaatc cggattggtc aggcagagtt ggccgtgatc gacgctccgt ttcccatcgc
     3181 gaaaactgta ccactctgga tgggccgcga aatgtgtctc gggaggcacc agcggataga
     3241 acgtgtgcac gaaccccttg taccggatgc acccgcccca atccgcgccg atgttggccg
     3301 actggctgtt cgagaaattg cgcaccgccc agcgcgcgtt gaacgccgga taccagaacg
     3361 gatcccgcga ctcgaacgtc gggcgatacc ggacatcgag tgcgccgacc gccaggacgg
     3421 cgtgcttcgg catctggctg gcccacggcg tccaccagcg caccccgcac tggtcctgga
     3481 ggaactgcga cacggcgtaa agcgtccctc gagggcggcc cccagccagc aacagacagt
     3541 tgcccttccg cttgatcaca atctcctcct cacccaactg atcgaaaggc acctccggaa
     3601 aaagcttcgc ggccaggctg ccgcacccga caacgatggc ccgttccggc gcctgatccc
     3661 ccgcctcctg gacgctgaac tgcgcgcccg tcacggcctc gagcgtctcg ctcaattccc
     3721 ggattgcgtt ttgttcggga agggtcgcgc cggcctggcg cacaatcaca cacgccgcct
     3781 tctcatcgcg cgccaattcg aggtcgactg ccgccagggc atcgagtgcg agtgcgaaac
     3841 cgagagccaa tagagttgtc ttcatgatgt gctatcgttg ggcccaccat agtcagccgc
     3901 agcacgagcg gcaaccaatt ccgctcacag cccaggcttg ccacacgacg cccttgaccg
     3961 gcacactccc ctcccaatgc cggatgcgat ccagacgcca tcggacctct gcctgacgcc
     4021 gcacggactg ctgcttgtca tggacgggcc gccgccttca ccctgggcgg acgccgagac
     4081 tggatgccgc gtggctcagg cattcgccga agccccggcg cgcggcctcc tgcacctcgc
     4141 cacgcgcgaa ctcaacgccg ctttgccccc gtccgccgcc tggtggcgcg aactggcccg
     4201 acgccatctc acccgggtct gccacatccc agacctcgat cagatgcagc agatcacccc
     4261 gattccgccg cccgccccgg acgaactcga cgcgttggcc gacaccgccc cgccgatgcg
     4321 tggcggcgaa tacttgaacg cggagatgct ggctagtctc tggaacggtt tggacgcgct
     4381 ggtgcgggcc gaagcgagtc ggcatccgcg cggggcgggc gcatggctga aggagagtca
     4441 ccccctctgg cgcatggtcg ggcgggtcac ctttcacctg gctgagaaca agcgtcaccc
     4501 gacccatccg tttgccttca tggccagcta tgcctcgcgc atttccgcgc aatcgcgcgt
     4561 gcagcatctg cccctggggc gcgcgctgca ggaatacgcc ggcgccggca acaaacccgc
     4621 gctggtcaac ttgctctcgc ccgtccagag agccgcggaa aagagccgct tcgtccgcga
     4681 actggtcgag accggccgca tcttcaaggc gctcccctgg actcccgccg aggcataccg
     4741 gttccttcaa gacacgcccg tgtgcgagca ggccggcgtc ctggtccggt tgcccgactg
     4801 gtggaagggc ggccgcccgc cgcgtccgcg cgtgagcgtg cgcgtggggc agaaccgcgg
     4861 cgccgggctg ggggtcgaca cgctgctgga tttctcggtc caaaccaccc tggacggcga
     4921 gccgttgacc gatgcggagt ggcagtcgct gctcgaagcg gccgaggggc tcgcgctggt
     4981 ccgcggcaaa tgggtcgagg tcgatcccgc aaaactgcgc gcggccctcg accattggaa
     5041 aaaagtcgag gccctcgccc ggcgcgatgg cctgagcttc ttcgagggca tgcgcatgat
     5101 cgccgggttt cgaccgggcg cggacgatgc cgctcaaacg ccggcctacg aagcggagcg
     5161 ggagtggtct gggattcaac ccggccagtg gctcgacacg gtgctggccg agttgcgcga
     5221 tccttcgcgc ttgggggctg cgtccaacat tcccgggctg cgcgcgcagc tccgccccta
     5281 ccaggcactg ggcgtgcact ggctcgagtt ccttgcccag ctcggcctgg gcggctgtct
     5341 ggcggacgac atgggcttgg gcaagaccat tcaggtgctg gctctggtgc tcaaacgcaa
     5401 aacggaagcc agaccgccgc accgccccaa cctcctggtc gtccccgcat cgctcctggc
     5461 caactggcgc gcggaaatcg aacggttcac accgacgctc tcgttcgcca tcatccatcc
     5521 ttccgaggca cacgaccccg cggccgcgct cgaatccgcc tccgacggag gcaccgacct
     5581 ggtcatcacg acctacggga tgctcgcacg cctcgagtcg ctgcgacgac gcgcgtggaa
     5641 tctggccatt ctcgacgagg ctcaggctgt caagaacgcc ggcacacgcc aggcccgcgc
     5701 ggtcaaggaa ctccaggccg ccgcccgctt cgccctcacc ggcacgccgg tcgaaaaccg
     5761 cgcggccgat ttgtggtcga tcttcgattt cctcaacccc ggcctcctcg gctcggccca
     5821 ggccttctcc cggttcgtcc gcgcccgggg cgattcgccc cagcccgatt acggaccgct
     5881 gcgggccctg acgcgtccgt acattctgcg gcggctcaag accgataagc gcatcattgc
     5941 ggacctgccg gacaagacgg aggtccgggc ctggtgcgcg ttgaccccgg cccaggccgc
     6001 gctttaccag aaggcagtga ccgagctggc cgaaggtctg gaggcggcgg acggcatcca
     6061 gcgccgcggc cttgtgctgg cattcatcat gcgcttcaaa cagatctgca atcacccgtc
     6121 gcagtggtcg ggcgacggcg gctacgcgcc ggaggcgagc ggcaagttcc aacgcctgcg
     6181 cgagttggtt gaggaaattg ccgagcggca ggagaaggcg ctggtgttca ctcaattccg
     6241 cgagatcacc gatccgctcg ccgccttcct ggccgaactc ttcgggcggc cgggcctggt
     6301 gctccacggc ggcacgcccg tcgcccagcg ccgcaagctg gtcgagtcgt tccatcggga
     6361 tgatgggccg cccttctttc ttctgaccgt gaaggcgggc ggggccggcc tgaacctgac
     6421 gcaggcggcc cacgtcatcc acttcgatcg ctggtggaat cctgccgtcg agaaccaggc
     6481 caccgatcgc gcgttccgca tcggccagaa gcgcaacgtg ctcgtgcaca agttcgtctg
     6541 tcgcggcaca atcgaggagc gcatcgatcg gttgatcgag tcgaaacaat ccctgtccag
     6601 ggaactcctc gaagggggag gcgaaacgct cttgactgaa atgagcaacg aagaactact
     6661 ccgtttcgtg gcgcttgatc tgaaccgcgc cactgaggca taaaccgtgc gcgcattatg
     6721 agttggtata gctacggatt ccgcccctac gtctcagtcg ccgagcggca acgcaaggcg
     6781 caacgggaaa tggacaggct cgccaagcag ggcaaggtca tccaccccat ccggctcgaa
     6841 ggccgcacca tcgcgcgcac cttctggggc aaggcctggt gcgacaacct cgaatcctac
     6901 atggactacg caaaccgcct gccccggggc cgcgcctatg tccgcaacgg ttccgtcgtc
     6961 cacctcgaga tcgggccggg cgagattcaa gccatggtca gcgggtcctc gctttacaag
     7021 gtccgcatcg ccattgcacc cgccgccaag cccaagtgga agaagctgtg ccaggaatgc
     7081 gccggcagca tcggttccct ggtcgaactc ctccaggggc gcttctccga ccatgtcatg
     7141 ggcatcttca cccgcaaaga caccggcctc tttccgaccc cggcggaaat caagctcaac
     7201 tgctcctgtc cggattgggc aaccatgtgc aaacacgtcg cggccgtgct ttacggcgtg
     7261 ggcgcccggc tcgaccagca gcccgatctg ctgttcaccc tgcgctccgt caaccaggag
     7321 gacctcgtca cccaggccgc aaccgccgcc gatctcaccg gcaagacccc gtccgccggc
     7381 cccgaactgg ccgagagcga cctcggcgca gtattcggga tcgagttgga ttcccaaacc
     7441 gccgcggtcc ccgctacacc cgccccagac gccccgcgcg ccaaggagcc gcgcagaagc
     7501 cgcaaggcgg ctacggccaa ggccaggagg ccggccaaag cgaaacgccg gaccgtcaaa
     7561 cggacttccc gatccgccaa acccagctaa acccccatgc aagactccag caacagcatc
     7621 tacaatctcg aggacttcgt cccaagcctg cgcgaggcgc tcgcggacca atcgcttgac
     7681 ctcgacgatt ccatgctcct ggccgccgtg ccgaccgcgg atggcaaccc ggacgacccg
     7741 ccccagcctg tcgaactgga tcgccgactg atccacctgg tggtcggcga caacctctgc
     7801 gagtgggacg tcgattcgct gcggtcgctg ttccgcggag atcgaaagcc cccgacactc
     7861 ggcgactacc ccgatgcgta taatgaagag ttcctgattc tcgaaataca cgtcctccaa
     7921 atcagcgata tcttcggcga ccggcgcgat gacgaaatgc gcgagatata ctcgctcctg
     7981 cgccgccgcc ccgacggcaa gagcatcgga ttcgtccaca actacatgtg gcaagccgcc
     8041 gcgatcatgc tcggcactcg ccccctcagc caggcggagt tcgaggcgat cctaagccgc
     8101 ctcgagcgct cctgccgcac gttctcgatg ggcccgggaa cgcggaacta cataaatgcc
     8161 ctgcgcggca tattcgaaca cgaagactcc cagccaagca aaaccgcgcc gagatagagg
     8221 gagagacgaa ggcaacggag tgtcggtaag agtctccttg aatcctcagc gtcctttatc
     8281 aaccgccaca ggggaagttt cagcccttgc gcaatgctat ccgccacgtt cacagaagga
     8341 ttccgatgga atcgctcaat cagggaaatc attttcgggg cttagtcggc tttctctgcc
     8401 aagtgttcct gcgtcagccc ttgggccttt cgatgtcgcc tcacgactgc cccgaaaagc
     8461 cgggcaaatt cccctgacaa catcctcgca tcctgccaac cgcagaagcc gcattgacag
     8521 gaacagatgt tccagtttcc acttgac
//
LOCUS       DAOQRO010000127         8495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_7868_length_8495_cov_6.098815, whole genome shotgun sequence.
ACCESSION   DAOQRO010000127 DAOQRO010000000
VERSION     DAOQRO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7868_length_8495_cov_6.098815"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..915
                     /locus_tag="PKK20_06350"
     CDS             130..915
                     /locus_tag="PKK20_06350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNU99540.1"
                     /translation="MRPLRLHWSSSKPNFGDWLSPILCEALSGRPVVHSSPARCDLIA
                     VGSILHRLPERWWARPVRVWGTGFIEERPRASSRHHYHAVRGHRTAALIDGPPPSAFG
                     DPGLLCDLVFSRPAPGGKEHAIGLVPHYKDRDHPWVKRFVERHPETRILNVFDEPRTF
                     LAQLVRCEFVISSSLHGLVVADAYGIPNVWMRLSGGIRGHDFKYLDYYSVFGFAEPPV
                     LTLENDVSGVALEALAQAYQRPGLEAVKKRLLDAFPFPAGPSP"
     gene            912..2066
                     /locus_tag="PKK20_06355"
     CDS             912..2066
                     /locus_tag="PKK20_06355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU99541.1"
                     /translation="MKLGFLLFDYFPYGGLQRDCLQIAARCAARGHAVTIFTRTWQGD
                     RCEGIEVELFGRRGFTNVGRNRAWLRQLASTLPRRQLDGIVGFNKLPGLDVYYGSDPC
                     YAAKVRRLKPAWSRWLPRYRHFARLESEVFERGRRTRILVLTDHEIPAYREFYDTEFE
                     RFHLLPPGIARRSVNEAQRQEARRRVREAQGWPLDERLLLLVGSGFRVKGLDRAIVAL
                     AALPPHLRRRSRLAVIGQNRPGAFALEARRLGVADRVCFLGGRLDVLDWLMASDLLVH
                     PAYSESAGMILLEAMASGLPVLATDTCGYAPHVTRARAGRILGSPFSQVECNRAVAEM
                     LVSSEQARWSANGLEYAAREDLYSCHDRAADLIEQIVQDNLAANARIAKP"
     gene            2063..2905
                     /gene="rfaP"
                     /locus_tag="PKK20_06360"
     CDS             2063..2905
                     /gene="rfaP"
                     /locus_tag="PKK20_06360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HNU99542.1"
                     /translation="MIELSPHLERHRPAHDAFEWLLNCPGKVHREVKHRRTVEVEIGG
                     RRFFIKAHRGCGWAEVLKDWVRGRAPIVSARSEWEAIDRARAVGIETLRVAAKGERGR
                     WPAAVESFVVTEALEGMITLESLTRTWGGLSGRRQVLFKRALLTKVARIARLMHGAGL
                     NHRDFYLCHFLVRERDWQQWQPSGSPADALDLVLIDLHRVQMRCRVPMRWLLKDLAAL
                     LFSALDCGLTTRDLLRFLKEYRQRPWREVLAAESGFWRRVSRRAGRLYAGFHGRPAPS
                     RRLA"
     gene            complement(2967..4385)
                     /gene="hemG"
                     /locus_tag="PKK20_06365"
     CDS             complement(2967..4385)
                     /gene="hemG"
                     /locus_tag="PKK20_06365"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNU99543.1"
                     /translation="MRADSVPRNPSELPPPVAVVGGGITGLTAAFRLMQAGVPITLYE
                     ADQRVGGVIRSVREHGYLAEFGPNSILETSPKISGLIRDLGLEPRRWYSDPGAENRYL
                     VRGGEPVCLPASPIRFFGTKLFSTGAKLRLLREPFLRRGPADKEENVAEFVLRRLGRE
                     WLDYAINPMIAGIYAGDPRRLSVIHAFPRLHAVEQRYGSLIVGQILGARERKRRGEVS
                     KQEAKKISFDQGLQVLTDTLEARLRAAIRLNTTATALKRTPRGWTVSVQSGGSTVAFE
                     HRAVLLAAPAHCLARIALDADAGLTLAPLTEVYYPPVASVVLGFRRADVDHPLDGFGM
                     LVPEVEKFRILGTLFSSSLFPNRAPDGHVTLTTYVGGVRAPDVALRPIEELFNLTLTD
                     LATILGVRGSPTFRHAVLYRQAIPQYEVGYGRFKTLMETIEARAPGIFLAGHYRNGIS
                     LGDSIIAGHDVADRIAAFARGQ"
     gene            complement(4539..5066)
                     /locus_tag="PKK20_06370"
     CDS             complement(4539..5066)
                     /locus_tag="PKK20_06370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU99544.1"
                     /translation="MNSTPDEVAVITTTEGDLVIALWPDVAPNTVANFKKLAQAGFYD
                     GTCFHRIVKDFMVQGGDPLTKDPARERSWGTGGPGYQIKAEFNQRPHVRGVISMARSQ
                     DLDSAGSQFFVCLADARFLDRQYTAFGELIRGDDVLGRIGDTPVTAGSDGERSKPIQR
                     VGVETIRIVPLDEAR"
     gene            complement(5063..5713)
                     /locus_tag="PKK20_06375"
     CDS             complement(5063..5713)
                     /locus_tag="PKK20_06375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU99545.1"
                     /translation="MKNMLLSVISGLAVCVATVLGQSDTATTKKEDAKATAPAVAEVA
                     VIETTKGRMVVEFWPDVAPKTVENFKKLARDKFYDGTCFHRIVKDFMIQGGDPLTKDP
                     AAQARYGTGDPGYKIKAEFNNRKHVRGVLSMARSAHPDSAGSQFFVCLGEESSPNMQY
                     LNGKYTAFGRLIKGEDVLLKIGETPTTMNAYGTERSVPTERIGVETVRIVPADSIK"
     gene            5883..6818
                     /locus_tag="PKK20_06380"
     CDS             5883..6818
                     /locus_tag="PKK20_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNU99546.1"
                     /translation="MNEHSASHLRGLPSFAHSNRRAFVRAAGLALGGLGLGATAARTA
                     EPTLTTASSAGTKSKMRLGLVTYNLAKDWDLPTIIKNCAAAKFEGVELRTTHAHKVEV
                     ALSKAERAEVKKRFEDSPVALMGLGSAFDYHTPDAAKLRKDIEATKEYIVLAYDVGAG
                     GVKVRPNGLPKEVPVEKTLEQIGKALLELGAFAAGYGQMIRLEVHGSGTSQLPHIRRI
                     MEAARHPNVGVCWNSNQSDLDGAGFDANFDSVKDRIVAVHMRDLYLEEYPWRRLMTRL
                     NAAGFAGYCLAEIPESADPLRVMHYYRALWLAYQA"
     gene            complement(6896..>8495)
                     /locus_tag="PKK20_06385"
     CDS             complement(6896..>8495)
                     /locus_tag="PKK20_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNU99547.1"
                     /translation="TTGLRSDVVTLYQGGLYHLYRFKRYTDVRLVFAPEQQVAFFGGD
                     PDNFEYPRYNLDICFFRVYENGKPAKVPHALRWSKAGAADGELVFVSGHPGRTDRMLT
                     VAELEYLRDVQYPYTLQLLHRREVVLEAWGQRNAENARKVRDDLFGVQNSRKAREGGL
                     AGLLDPAFIASKQAAEQKLRAAVSADARFQEARDAWDRIAAAQAVIAEHARMYRLLEA
                     GHGFNSEWFGVARTILRAAEEHPKPDGERLREFRDSNRESLELDLFSTRPVYDDYEQV
                     RLADSLTWLVEQLGYSNPLVQRVLDGKSPRQRALDLVRGTTVKDVARRKALYNGGKSV
                     VDAASDPMIDLARSIDTEARALRKTLETQDEIKRQAHAQIAKARFAIDGASTYPDATF
                     TLRLAFGQVKGYEEAGRHVPFQTTFAGLYQRWKDQQGLPPFDLPPRWLKRKSALNLDT
                     PLNFVCTADIIGGNSGSPVVNRAGEFVGIIFDGNIQSLVLDFYFTDDVARAVSVHSSA
                     ILESLRRVCGAKALADELVAGRLK"
BASE COUNT         1429 a         2605 c         2904 g         1557 t
ORIGIN      
        1 gggtgtcacg gtcgacgttc acgaagcgtg ggcccatagt tatctgctgg actctgaacc
       61 tcgccctctt gttcaaacgc cgggcaaaat tgttggggaa aagtccgata ggctgctaga
      121 gtcgtgcgca tgcgtcctct cagactccac tggtcgagca gcaagcccaa tttcggggat
      181 tggctttcgc ccatactctg cgaggcgctg tccggacgcc ccgtggtgca cagcagtccg
      241 gcccggtgcg acctgattgc cgtcggcagc attctccacc gactgcccga acgctggtgg
      301 gcgcgtccgg tgcgcgtttg gggaaccggg ttcatcgagg agcggccccg ggcctcgagc
      361 cgacaccact atcacgccgt gcgggggcac cggacggcag cgctgatcga cgggcctcct
      421 ccgtcggcct ttggcgatcc gggcctgctg tgcgacctgg tcttttcacg acccgccccc
      481 ggtgggaaag agcacgcgat cggcctggtg ccgcactata aggaccgcga tcatccgtgg
      541 gtgaagcggt ttgttgaacg acatccggag acgcggatat tgaacgtctt tgacgagccg
      601 cggacgttcc tggcccaact ggtccgttgc gagttcgtca tttcatcgag tctgcatggg
      661 ctcgtggttg cggacgcgta tggaatcccg aatgtctgga tgcggctgtc ggggggcatc
      721 cggggccatg atttcaagta tctcgattac tacagcgtgt ttggattcgc cgagccgccg
      781 gtgctgaccc tcgagaacga tgtgagtggg gtggcgctcg aggccctggc ccaggcctat
      841 caacggccgg ggctcgaggc cgtgaaaaag cggcttctcg acgcgttccc gttccccgcc
      901 gggccatcgc catgaaactg ggtttcctcc tgttcgatta ctttccttac ggaggactgc
      961 agcgggattg cctccagatc gccgcgcgtt gcgccgcgcg cgggcacgcg gtaacgatct
     1021 tcacgcgcac ctggcagggc gatcgatgcg agggaatcga agtcgaactg ttcggacgac
     1081 gcggcttcac caacgtcgga cgcaaccggg cctggctgcg gcaactggca tcgacgctgc
     1141 cccggcgcca gctcgatggc attgtcgggt tcaacaagtt gcccgggctg gatgtgtatt
     1201 atggctcgga cccgtgttac gcggcaaagg tgcgtcgcct caagcccgca tggtcacgct
     1261 ggctcccgcg gtaccgccat ttcgcccggc tcgaatccga agtcttcgag cgcggacgcc
     1321 ggacccgaat cctggtgttg accgatcatg aaatcccggc ttatcgggaa ttctacgaca
     1381 ccgagttcga gcgttttcat ctgctgccgc ccggcattgc ccggcggtcc gtgaacgaag
     1441 cgcagcgcca ggaggcgcgc cgcagggtgc gagaggcgca aggctggccg ctcgatgagc
     1501 gtctgctgct gctggtgggc tcgggctttc gcgtcaaggg actcgatcgc gcgattgtgg
     1561 cgctggcggc cctgcccccg catctccgcc ggcgaagccg gttggcggtg atcgggcaga
     1621 atcgtccggg cgccttcgcc ctcgaggcgc ggcgcctggg tgttgccgat cgtgtttgtt
     1681 tcctgggcgg acggctggat gtgctcgatt ggctgatggc gtccgatctc ctggtgcatc
     1741 cggcctacag cgaatcggcc gggatgatcc tgctcgaggc gatggcgtcg ggcctgcccg
     1801 tgctggccac ggacacctgc ggctatgccc cgcacgtgac gcgggcgcgg gccgggcgta
     1861 tcctgggctc gcccttttct caagtcgagt gcaaccgcgc ggtggccgag atgcttgttt
     1921 cgagcgaaca ggcgcggtgg agcgccaacg ggctcgagta tgcggcgcgc gaggatctgt
     1981 acagttgcca cgacagagcc gcggatctga tcgagcagat cgttcaggac aacctggcgg
     2041 cgaacgcgcg catcgccaag ccgtgattga actgagtcca catcttgaac gccaccggcc
     2101 cgcgcacgac gccttcgaat ggctgttgaa ctgtccgggc aaggtccatc gcgaggtgaa
     2161 acatcggcgc accgtcgagg tcgagatcgg cggacgccgc tttttcatca aggcgcatcg
     2221 cggatgcggt tgggcggagg tgctcaagga ctgggtccgc ggccgggcgc cgattgtgag
     2281 cgcccgttcc gagtgggagg cgatcgaccg ggcgcgggcg gtggggattg aaacgctccg
     2341 ggtggcggcc aagggcgaac gtgggcggtg gccggcggcg gtggaatcgt ttgtcgtcac
     2401 cgaggcgctc gaaggcatga tcacgctcga aagtctcacg cggacctggg gcgggttgag
     2461 cgggcgcagg caggtgctct tcaagcgggc gctgctgacg aaagtcgcgc gcatcgcgcg
     2521 cctgatgcac ggggccgggc tcaaccaccg cgacttctac ctctgccact tcctggtgcg
     2581 cgagcgcgac tggcaacaat ggcagccgag cggctcgccc gccgacgccc tcgacctggt
     2641 gctgatcgat ctgcaccgtg tccagatgcg ttgccgcgtc ccgatgcgct ggctgctcaa
     2701 ggacctggcg gcgttgcttt tctccgccct ggactgcggc ctcaccacgc gcgacctgct
     2761 ccggttccta aaggaatacc ggcagagacc gtggcgagag gtgctggccg ccgaaagcgg
     2821 tttctggcga cgcgtctcga ggagagccgg acggttatac gccggattcc acggacggcc
     2881 ggcgccgagc cggcgccttg catagcttca gcccggatgg ttcgtctgcc ggccggcagg
     2941 cagattctgg cccctgcgag actggttcac tgtccccgcg cgaacgccgc gatccgatcc
     3001 gcaacgtcat ggccggcgat gatcgagtcg cccagcgaga tgccgttgcg ataatgcccg
     3061 gccagaaaga tgccgggcgc gcgcgcctcg atcgtttcca tgagggtctt gaagcgcccg
     3121 taaccgacct cgtactgggg gatggcctgg cgatagagca cggcgtgtcg aaacgtggga
     3181 ctgccgcgga caccgaggat cgtcgcgaga tcggtgagtg tgagattgaa cagttcctcg
     3241 attgggcgca gggccacatc cggcgcccgc acaccgccga cgtaggtggt gagagtcacg
     3301 tgcccgtccg gggcgcgatt cgggaacagc gaggatgaga agagcgtgcc gaggatgcgg
     3361 aacttctcga cttcgggcac caacatgcca aagccgtcca gcgggtggtc gacatccgcg
     3421 cggcggaagc cgagcaccac gcttgcgacg ggcgggtagt agacttcagt caaaggcgcg
     3481 agcgtcaggc cggcatcggc atcgagcgcg attcgggcca ggcagtgcgc cggagcggca
     3541 agcaggacgg cccgatgctc gaaggcgacc gtcgatccgc cgctttggac cgacaccgtc
     3601 cagccgcggg gcgtgcgttt gagggcggtc gccgtcgtgt tgagacggat ggctgcgcgc
     3661 aacctcgctt cgagcgtatc ggtcaacacc tgcaagccct ggtcgaacga gattttcttc
     3721 gcttcctgct tcgagacttc gccgcgtcgc ttgcgctcgc gcgccccgag aatctgtccg
     3781 acaatgagag agccatagcg ttgctcgacg gcgtggagac ggggaaaggc atggatgacc
     3841 gagagccggc ggggatcgcc tgcatagatt ccggcgatca tcggattgat ggcgtagtcg
     3901 agccattccc gacccagccg gcgcaggacg aactcggcga cattctcctc cttgtccgcc
     3961 ggaccgcggc gcagaaacgg ctcgcgcagc aggcgcagtt tcgcgccagt cgagaacagt
     4021 ttcgtgccga agaacctgat gggcgaggca ggcaggcaga ccggctcgcc gccgcgcacc
     4081 agatagcgat tctcagctcc cgggtccgaa taccatcggc ggggttcgag gccgagatca
     4141 cggatcaagc cgctgatctt gggcgaggtt tcgagaatcg aattggggcc gaactcggcc
     4201 agatatccgt gctcgcgcac ggaacggatg acgccgccga ctcgttgatc ggcttcgtag
     4261 agcgtgatcg gcacgcccgc ctgcatcagc cgaaaggcgg cggtcagacc ggtgatgccg
     4321 ccgcccacga cagcaaccgg cggcggcaat tcggaggggt ttcgtgggac tgagtcagcc
     4381 cgcatagttc ttagcgaggc tccgcctcaa accggcgagg cacatggcca gatcgtttgg
     4441 actgggcgtt cgcggtcgtc actggcaagc gaggccttgc taacccggaa atctcaccag
     4501 tgggtcgatc acaggctgcg cgctgaacct ggtccgggct agcgcgcctc atcgaggggc
     4561 acgatgcgga tggtctcgac gccgacgcgc tggatcggct tgcttcgttc gccgtcgctg
     4621 ccggccgtga cgggcgtgtc gccgatgcgc ccgaggacgt cgtcgccgcg gattaactcg
     4681 ccgaaggccg tatactggcg gtcgaggaac cgggcgtcgg cgaggcagac gaagaactgg
     4741 ctgccggccg aatccagatc ctgggagcgg gccatcgaga tgacgccgcg cacatgcggg
     4801 cgttggttga actcggcctt gatctggtag ccggggccgc ccgtgcccca ggagcgctcg
     4861 cgcgcgggat ccttggtgag gggatcgccg ccctgcacca tgaagtcttt aacgatgcgg
     4921 tggaagcagg tcccgtcgta gaagccggcc tgcgccagtt tcttgaaatt ggccacggtg
     4981 ttcggggcta catcgggcca taacgcgatg accaggtcgc cttcggtggt cgtgatcacg
     5041 gccacttcat ccggggtgga gttcatttga tggaatctgc tgggacgatg cgcacggtct
     5101 cgaccccgat ccgctcggtg gggacgcttc gttcggtgcc gtaggcgttc atcgtggttg
     5161 gggtctcccc gatcttcagc aacacgtcct cgcctttgat gagcctgccg aaggcggtgt
     5221 acttgccgtt gagatattgc atgttgggag aactctcctc gcccagacag acgaagaact
     5281 ggctgccggc cgagtccgga tgggccgagc gggccatcga gagcacgccg cgcacatgct
     5341 tgcgattgtt gaactcggcc ttgatcttgt agcccgggtc gccggtgccg tagcgggcct
     5401 gggccgccgg gtctttggtc agcggatcgc cgccctggat catgaaatcc ttcacgatcc
     5461 ggtgaaaaca ggtgccatcg tagaatttgt ccctggccag cttcttgaaa ttctcgactg
     5521 tctttggcgc aacgtccggc cagaactcga cgaccatcct gcctttggtg gtctcgatga
     5581 ccgcgacttc ggcgacagcg ggggcggtcg ccttggcgtc ctctttcttc gtcgtcgctg
     5641 tgtcggattg acccaggacc gtcgcgacgc atacggccag accgctgatc acgctcaaca
     5701 acatgttctt catgcaggac agcttggctt gaccatgccg ccgctgtcac gcccggattt
     5761 tgagcgcacc accgggaaca cagagttggg gagttcaatt tcgtccaggg gtgctcgcaa
     5821 tcgacgcaac tgttgcaatc ctttgacacc gggggagacg ttggcccatc ctcggggtcg
     5881 tcatgaacga gcactccgca tcccatctcc gtggcttgcc ctctttcgcg cactcgaacc
     5941 gccgcgcttt cgtccgagcc gccggcctgg cgttaggcgg actcgggctc ggcgcgaccg
     6001 ccgcccgaac tgcggaaccc acgctgacca cagcgtcctc tgccggcaca aagtcgaaga
     6061 tgcggctggg actggtcacg tacaatctcg ccaaggactg ggatctgccg accatcatca
     6121 agaactgcgc ggcggcgaag ttcgaggggg tcgagttgcg gaccacgcac gcgcacaagg
     6181 tcgaggtggc gttgagcaag gccgagcgcg ccgaggtgaa gaagcgattc gaggactcgc
     6241 cggtggcgct gatgggtttg gggagcgcgt tcgattacca cacgccggac gccgccaagc
     6301 tgcggaaaga catcgaggcc accaaagagt acatcgtcct tgcgtacgac gtgggggccg
     6361 gcggcgtgaa ggtgcgtccc aatggcctgc ccaaggaggt gcctgtcgag aagacgctcg
     6421 aacagattgg gaaggccctt ctcgaattgg gcgcgttcgc cgcgggctac ggccagatga
     6481 tccggctcga agtgcacggg tcgggcacgt cccaattgcc gcacatccgc cgcatcatgg
     6541 aggccgcccg gcacccgaat gtcggcgtgt gctggaactc gaatcagagc gatctcgatg
     6601 gcgcgggttt cgacgccaat ttcgattcag tcaaggaccg gattgtggcg gtgcacatgc
     6661 gcgaccttta cctcgaagag tatccgtggc ggcggctaat gacgcggctg aacgcggcgg
     6721 ggtttgccgg ttactgcctg gcggagatcc ccgagagcgc tgatccgttg cgcgtgatgc
     6781 attactatcg cgccctgtgg ttggcgtatc aggcctaacc cgcatagtgt gcacccagtc
     6841 agtgcacagg acagtcgtgt ctcgcaagag gactactatg cgggttagaa acctctcact
     6901 tgagccggcc ggcgaccaac tcgtccgcca atgccttggc cccgcagacg cgccgaagcg
     6961 attcgaggat ggctgaggaa tggaccgaca cggcgcgcgc gacgtcgtcg gtgaagtaga
     7021 agtccaggac cagggactgg atattgccgt cgaagatgat cccgacgaat tccccggcgc
     7081 ggttgacgac gggactgccg gaattgccgc cgatgatatc cgcggtgcag acgaaattga
     7141 gaggggtatc gaggttcagg gcgcttttgc gcttgagcca gcggggcggc aggtcgaagg
     7201 gcggaagtcc ctgctgatcc ttccagcgct ggtagaggcc ggcgaaggtt gtttggaatg
     7261 gcacgtgccg tccagcctcc tcgtatcctt tgacctggcc gaacgccaat ctgagcgtga
     7321 aggtggcgtc ggggtaggtg ctggcgccgt cgatggcgaa ccgggctttt gcgatttgcg
     7381 cgtgggcctg acgcttgatt tcatcctggg tctcgagcgt cttgcgcaat gcgcgcgcct
     7441 cggtgtcgat cgacctcgct aggtcgatca tcggatcgga cgcggcgtcc acgacgctct
     7501 tgccgccgtt gtagagtgcc ttgcgccggg cgacgtcctt gaccgttgtg ccgcggacga
     7561 ggtcgagagc gcgctgtcgg ggcgacttgc catcgaggac gcgttggacc aggggattcg
     7621 agtagcccaa ttgctcgacc agccacgtga gggaatcggc gagccggacc tgttcgtaat
     7681 cgtcgtagac ggggcgggtc gagaacagat caagttcgag cgactcgcgg ttcgagtcgc
     7741 ggaactcgcg caggcgctcg ccgtccggct tggggtgttc ctcggccgcc cgcaggatcg
     7801 tgcgggcgac gccaaaccac tcgctgttga atccgtgccc ggcttcaagc aggcgataca
     7861 tccgcgcgtg ctcggcgatg accgcctgcg ccgccgcaat gcggtcccag gcgtcgcgag
     7921 cctcctggaa acgggcgtcg gccgacacag ccgcccggag cttctgttcg gcggcctgtt
     7981 tggatgcaat gaatgccgga tcgagcaggc cggccaggcc gccttcgcga gccttgcggc
     8041 tgttctggac gccgaacagg tcgtctcgga ccttgcgggc gttctcggcg ttgcgctgac
     8101 cccatgcctc gagcacgacc tcgcgccgat gcagcaactg gagggtgtac gggtactgca
     8161 cgtcccgcag atactcgagt tcggccaccg tcagcatccg atccgtgcgc ccgggatggc
     8221 ccgacacgaa caccagttcg ccgtccgcag cgccggcctt gctccagcgg agggcgtggg
     8281 gaaccttggc cggcttgccg ttctcgtaga cgcggaagaa gcagatatcc aggttataac
     8341 gcgggtactc gaaattgtcc ggatcgcctc cgaagaaggc gacctgctgt tcgggggcga
     8401 acacgaggcg gacgtcggtg taacgtttga agcgatagag atggtagagg ccgccctgat
     8461 agagcgtcac cacgtcgctg cgcaggcccg tggtg
//
LOCUS       DAOQRO010000128         8318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8115_length_8318_cov_4.944088, whole genome shotgun sequence.
ACCESSION   DAOQRO010000128 DAOQRO010000000
VERSION     DAOQRO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8115_length_8318_cov_4.944088"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKK20_06390"
     CDS             <1..992
                     /locus_tag="PKK20_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99548.1"
                     /translation="AGLKAIVSDQRVSGYDWRKVDAGVARTKVAALIAETGSHPAVYG
                     YYLRDEPGAEAFPGLATVASIVKELAPGKWPYINLFPNYASAAQLGTATYEEHLEKFV
                     ATCRPTHLSYDHYALMEDGSLRAGYFLNLEQMRQAAKKHGLPFWNIVLSVAHFNYREA
                     TPADLRFQVYTTLAYGGRGIAYFTYFTPQVGNYRMAPIDQFGHATPTWSAMQNVNLQV
                     GKLAPTLLQLTSNEVYHFGAIPEGAHGPGESSLIAAVGNAPFMVGDFTHADGSRYALV
                     VNKDSLRSHHGAVTFRQNPRRVQMVSPYTGQLIGFTGEQQWLAAGQGVLLKVE"
     gene            1206..2441
                     /locus_tag="PKK20_06395"
     CDS             1206..2441
                     /locus_tag="PKK20_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99549.1"
                     /translation="MAPASILAQGARPAPSERIVLAAIGVGSRGVRNLESFLGESDCQ
                     VMAVCDVDKLHAEEGKELVNRHYKNQDCRVYYDFRHLLARADLDAVALALPNHWHAVP
                     AIQAVKAGLDVYSEAPLAHTLREGRAICEAVRRYGCIWQTGSWQRSVETFRTAAQLVR
                     NGRIGKVRAVEVGVPSGHPDLCGTAGQEQAGPPPTHLDYEFWLGPAPWAPYCPARVHQ
                     NWRWHLDYGGGQLLEWVGHHVDIAHWGMDWDATGPVEVEAAGEFPSRGLWNTATAFRV
                     TCKYANEVSMLIAAGAPQVRIGTRWIGEDGWIWCDRETIEAEPKRLLLEPTDAMKIKL
                     PGSPGHHRNFLDCVKSRRAPVAPAEVGQRSMTPGHLGQIAMQLRRKLSWNPETETIAD
                     DPTASRLLGTAMREPWRLG"
     gene            complement(2622..2915)
                     /locus_tag="PKK20_06400"
     CDS             complement(2622..2915)
                     /locus_tag="PKK20_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99550.1"
                     /translation="MFSSRTLGELETRRQSLIIAAADHRARLHHDWSRLADSLSWLDR
                     AVHWAREARPLLWIAAPMAGYTLARRGRVLLRWLPLIWPWWRIAKSLGARFRQ"
     gene            complement(2894..3283)
                     /locus_tag="PKK20_06405"
     CDS             complement(2894..3283)
                     /locus_tag="PKK20_06405"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNU99551.1"
                     /translation="MATEPKSPQGFFASLLGAIESVLALARTRFELLTVELQQEKVRL
                     IDLLLRVAAAAILALLALGTLTAGLIWLLWPRSPIAAFVGLTLLYGGGSAWLLIGIRR
                     ELKHGLKPFAGTLAEFEKDRACFRVGR"
     gene            complement(3288..3608)
                     /locus_tag="PKK20_06410"
     CDS             complement(3288..3608)
                     /locus_tag="PKK20_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNU99552.1"
                     /translation="METYFHNIEEPAKISREKVAEDLRVLIRDAEELLKAGVGDTSGK
                     TAEIKARLQASVDKAKATATQLEDRALAGAKAADRLIRDHPYESIGASFGVGLLFGLL
                     LGRR"
     gene            complement(3725..4123)
                     /locus_tag="PKK20_06415"
     CDS             complement(3725..4123)
                     /locus_tag="PKK20_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99553.1"
                     /translation="MRQFLTVVMVVALVWLGWKFSHYAKKEIATTPVRQDGGQAPAPG
                     KLPGMAASVEESYEAAKREGAQGVGRWLAQHQSEIQDPRLTDIELDYVLLVGRSNVAE
                     ARRVLAEIRQRITPASPVYKRFEQLSKTYE"
     gene            complement(4120..5382)
                     /gene="murA"
                     /locus_tag="PKK20_06420"
     CDS             complement(4120..5382)
                     /gene="murA"
                     /locus_tag="PKK20_06420"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNU99554.1"
                     /translation="MYSLLIEGGRPLEGEVTISGAKNAVLPLMAAALLTREPCVIRRV
                     PALSDVAFMVRILTAVGAKVRSQGDTVSIEANRIRGFCDYDLVRKMRGSVCLLGPLLG
                     RLGQAKVSLPGGCVIGTRPIDLHLKGLRALGAKIQIDSGYVCAQTRRLKGSAMFLGGR
                     AGPTVLGTANVMMAATLAEGVTVIQSAACEPEIVDLAEFLNAMGAQITGAGSPTITIT
                     GVKELHGAEHEAIPDRIEAATFAIAAACSRGEVTLRGARPEHLHAVLDKLEEAGVRLD
                     RAGGNLTVKGGRRLRPVDVTTQPYAGFPTDAQAQMMVLMTQASGISILTERIFETRFM
                     HVSELARLGADITIEGPSAVVKGPSVLSGAPVMASDLRASAALVIASLVARGRTHVKR
                     IYHLDRGYERIDDKLRRLGARIQRLSDS"
     gene            complement(5477..6337)
                     /gene="prmC"
                     /locus_tag="PKK20_06425"
     CDS             complement(5477..6337)
                     /gene="prmC"
                     /locus_tag="PKK20_06425"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNU99555.1"
                     /translation="MTVLEVIRRSTDYLAQKGVDSPRLQIELLLAHVLKLPRLQLYLN
                     FERTLGESDLATVRALVRRRGNREPLQHLTGLVSFCGFELAVGPDVLIPRPETEVLAE
                     RAWTLLETLPVAAPAVLDFGTGSGCLAIALARHCPAAHITALDISEPALVRARANAER
                     QGVADRIEWARGDGLAAVRPGLHFDVIVANPPYIPSVEIERLAPEVRDHDPRPALDGG
                     PDGLDVIRQLAAGAAARLNRSGHLLVEFGDGQATAVSDLFVRHGWIVEAVEADLSGRE
                     RILIARPARR"
     gene            6575..7450
                     /gene="argB"
                     /locus_tag="PKK20_06430"
     CDS             6575..7450
                     /gene="argB"
                     /locus_tag="PKK20_06430"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNU99556.1"
                     /translation="MQDLISKAATLLEALPYLQKYSGATFVVKYGGSFMDSPDPVIRT
                     SVARDIVFLEAAEINPVVVHGGGKAITRAMEKAGLKAQFVQGQRVTDEATVRVVDAVL
                     SGEINPEVVTTISSLGGLARGFAGTEVFTCRKLLVHGPDGQPLDIGFVGEVTGVNTAP
                     LLACIEKGITPVISPTARDKDGQVYNCNADVAAARAAIALRARRLVFMSDVPGLLRDP
                     KDPATVIPHLRVSEVDALKHAGIVERGMIPKVDSAVAAIRAGVDKVSFVDGRVPHAVL
                     LEIFTDEGVGTEVVL"
     gene            7515..>8318
                     /locus_tag="PKK20_06435"
     CDS             7515..>8318
                     /locus_tag="PKK20_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNU99557.1"
                     /translation="MKDIIPPPPIIANQFETVRELFQKYVVPSYGRFDLAFARGAGSF
                     LCDVSGKRYLDLGSGIAVCSLGHAHPEITEALIEQSRKMVHISNLYYHEPQGRLAEQI
                     VRLLAPGKVFFANSGGEANEGLFKLARKFGHDEGRFEILTALNSFHGRTLAGIAATGQ
                     DKVKRGFEPMVAGFRHVPFNDLEAMRQALSPATVAILIEGVQGEGGITPASPEYLLGL
                     RALCDEKRLLLLMDGVQCGYFRTGRFQSFQRILEGVSGADGFLPAGVSMA"
BASE COUNT         1451 a         2715 c         2738 g         1414 t
ORIGIN      
        1 cggcggggct gaaggctatc gtctccgacc agcgcgtctc cggctacgac tggcgcaagg
       61 tggacgccgg ggtggcgcgg acgaaggtcg ccgcactgat cgccgagacc ggatcgcatc
      121 cggcggtgta cggctactac ttgcgcgatg agccgggggc ggaggcgttc ccgggactgg
      181 ccacggtggc gtccatcgtg aaggagctgg ctccgggcaa gtggccctac atcaatctct
      241 ttcccaacta cgccagcgcg gcgcaactcg gcaccgccac ctacgaagag catctcgaga
      301 agttcgtcgc cacctgccgg ccgacgcatt tgagctatga ccactacgcg ctgatggagg
      361 acggctcgtt gcgcgccggg tactttctga acctcgagca gatgcgccag gccgccaaga
      421 aacatggcct gccattctgg aacatcgtcc tgagcgtggc ccacttcaat taccgtgagg
      481 ccacccctgc cgacctgcgc ttccaagtct acaccaccct cgcctacggc gggcgcggca
      541 tcgcctactt cacctacttt acgccccagg tgggcaatta ccgcatggcg cccatcgacc
      601 aattcggcca cgccacgccc acatggtcgg caatgcagaa cgtcaacctc caggtcggca
      661 agctcgcccc gaccctgctg caactgacct cgaacgaggt ctatcatttc ggggcgatcc
      721 ccgaaggcgc acacggcccc ggggaatcga gcctgatcgc agccgtcgga aacgcgccgt
      781 tcatggttgg ggatttcacg cacgcggacg gcagccgcta cgcgctcgtc gtcaacaagg
      841 attccctccg atcccaccat ggcgccgtca cgttccgcca aaaccctcgc cgcgttcaga
      901 tggtctcgcc ctataccgga cagttgatcg ggttcacggg cgaacaacag tggctggccg
      961 ccggacaggg agttctgttg aaagtcgagt agctcggccg gcgcaaggcc cgcgcagggc
     1021 ggccctgccc ggatgttcgg caaagcttcc gatccatgcg gacgcacgcc gacggcgccg
     1081 gagatatcga acgatttgca cgaatgatca gagacgaaca tcgagtgtgc gcgccccatc
     1141 gatccacgcg ccgccgcttt ctgcaaacgc tcgcgacggc cccgaccctt cttgccgtcc
     1201 ctatcgtggc gccagcctcc atcctggccc agggcgcgcg tcccgcccca agcgagcgca
     1261 ttgtcctggc cgccatcggc gtcggctcga gaggggtccg caatctcgag tcgtttctcg
     1321 gagagtcgga ctgccaggtg atggccgtgt gcgatgtgga caagctgcac gccgaagaag
     1381 gcaaagaact ggtgaaccgg cattacaaga accaagactg ccgcgtctat tacgatttcc
     1441 gccatctcct ggctcgggca gatctggacg cggtggcgct ggccttgccc aaccactggc
     1501 acgccgtccc tgccattcaa gcggtcaaag ccggactgga cgtctacagc gaggcgcccc
     1561 tggcccacac tcttcgagaa ggcagggcga tttgcgaagc tgtccggcgg tatggctgca
     1621 tctggcagac cgggagctgg cagcgctcgg tcgaaacctt ccggacggcc gcccaactcg
     1681 tgcgcaacgg gcgcattgga aaagtgcgcg cggtcgaggt tggtgtgccg tcgggacacc
     1741 cggacctctg cggcaccgcc gggcaggaac aggcgggacc gccacccacg cacctcgact
     1801 acgagttctg gctcggaccc gctccgtggg caccctattg tccggcgcgc gtccaccaga
     1861 actggcgctg gcacctggat tacggcggag gccaattgct cgaatgggtg ggccaccacg
     1921 tcgacatcgc gcactggggc atggactggg acgcaaccgg tccggtcgag gtcgaggcgg
     1981 cgggcgagtt tccgtcacgc ggtctgtgga acacagccac ggcgtttcgc gtcacctgca
     2041 agtacgcaaa cgaggtctcg atgctgattg ccgcgggggc cccccaggtg cgcatcggca
     2101 cgcgctggat tggcgaagac gggtggatct ggtgcgaccg cgagacgatc gaagccgaac
     2161 cgaaacggct gttgctcgaa cccacggacg cgatgaagat caagctgccc ggctctccgg
     2221 gccatcatcg caactttctt gattgcgtga aatcgcgtcg cgccccggtc gcgcccgcgg
     2281 aggtcgggca gcgctcgatg acccccggtc acctgggaca aatcgccatg cagctccggc
     2341 gcaagctgtc ctggaacccg gagacagaga caatcgccga cgacccgact gcgtcgcgtc
     2401 ttctgggaac ggccatgcgc gagccgtggc ggctggggtg agagaagccg ggccctgcgg
     2461 aggacaagga ccaatgcaat ccctcagtcg tttctatctg tacgccgccc tcgcgctccg
     2521 ccgccctcgc gctcacgttg ccggctcagc agcccctcgg ttggggcatc gaagtgggca
     2581 gcgacctggc agtgctcaag aaaaaggctc tgtgcaaaat actactgtcg aaaacgcgct
     2641 cccaagcttt tggcgatgcg ccaccagggc cagatcaagg gaagccaacg caggaggaca
     2701 cgcccgcgtc tggccagggt gtaaccggcc atcggagcgg cgatccagag cagcggacgc
     2761 gcctcgcgcg cccagtgcac ggcgcgatct agccagctca aggaatcggc caggcgactc
     2821 cagtcgtggt ggagtcgggc gcggtgatcc gcggcggcga tgatgagcga ttgccgtcgg
     2881 gtctcgagtt cgcctaacgt ccgactcgaa aacatgcgcg gtccttttcg aactccgcca
     2941 gggtgccggc gaatggcttg agcccgtgtt tcaactcgcg tcggatgccg atcaggagcc
     3001 aggccgagcc gccgccatag aggagggtca agccgacgaa cgcagcgatg ggcgaccttg
     3061 gccagagcag ccagatgagg ccggccgtga gcgtgcccag ggccagcagc gcgagaatcg
     3121 cggccgcggc cacgcgcagg aggagatcga tcaaccggac cttctcctgc tgcaactcga
     3181 ccgtcaacag ctcgaagcgc gttcgcgcga gagccagcac ggactcgatc gcgccgagca
     3241 acgaggcgaa gaagccttga ggcgacttcg gttcggttgc catgaggcta tctgcgtccc
     3301 agcaacaggc cgaacaacag gccgacgccg aaagacgcgc cgatggattc gtacgggtgg
     3361 tcgcggatca ggcggtcggc cgccttggcg ccggcgaggg ctctgtcttc gagttgagtg
     3421 gcggtggctt tagccttgtc gaccgaggcc tggaggcggg ctttgatttc cgcggtcttg
     3481 ccgctggtgt cgcccacgcc cgccttgagc aactcctcgg cgtcgcggat gaggacgcgg
     3541 agatcctcgg cgactttttc gcggctgatc ttggccggct cttcgatgtt atggaaatag
     3601 gtttccatgg tcatgtccgt atctttcact tgttgcggcg gcgggctcgc cggtgggaga
     3661 gccccacgtc gcccagactc aacttggagc gcgaaacggc gcgagtcgag ccgaaagttg
     3721 aacgttattc gtacgttttc gaaagctgct cgaaccgttt gtagaccggg gatgcgggcg
     3781 tgatgcgctg ccggatttcc gccagaacac gccgggcctc ggcgacattc gagcgcccga
     3841 ccaacagcac ataatccagc tcgatgtccg tcagccgcgg atcctggatt tcgctttggt
     3901 gctgggccag ccatcggccc acgccctggg ccccctcgcg tttggccgcc tcataactct
     3961 cttcgacgct ggcggccatg cccggcagtt tgcccggggc gggcgcttgc ccgccgtctt
     4021 gccgcaccgg ggtggtggcg atttcctttt tggcgtagtg gctgaacttc cagcccagcc
     4081 agaccagggc cactaccatg acaacggtca ggaattgtct catgagtcgg agagtcgttg
     4141 aatccgggcg ccgagccgac gcagtttgtc gtcgatgcgc tcgtagccgc ggtcgaggtg
     4201 gtaaatgcgt ttcacgtgcg tccggccccg ggcgacaagg ctggcgatca ccagcgcggc
     4261 cgaagcgcgc aggtcgctcg ccatcaccgg cgcgccgctc aagaccgagg ggcccttgac
     4321 gacggcgctg ggtccttcga tcgtgatgtc ggcgcccagc cgcgccaatt cgctcacgtg
     4381 catgaaccgg gtctcgaaga tgcgctcggt gaggatgctg atgccggacg cctgggtcat
     4441 taacaccatc atctgcgcct gggcgtcagt cgggaacccg gcgtacggct gcgtggtgac
     4501 atccacgggg cgcagccgcc gcccgccctt gaccgtcaag ttcccgccgg cgcgatcgag
     4561 tcgaacgcct gcttcctcga gcttgtcgag gaccgcgtgg agatgttcgg gacgcgcgcc
     4621 ccgcagggtc acttcgccgc gcgaacacgc cgccgcaatg gcaaaggtcg ccgcctcgat
     4681 ccgatccggg atggcctcgt gctccgcgcc gtgcagttcc ttgacgccgg tgatcgtgat
     4741 ggtgggactg ccggcgcccg tgatttgcgc ccccatcgca ttgaggaatt cggccaggtc
     4801 aacaatctcc ggttcgcagg cggcgctctg gatcaccgtc accccctcgg ccagcgtcgc
     4861 ggccatcatg acgttggccg tgcccaaaac ggtcgggcca gcgcgcccgc ccaggaacat
     4921 ggccgagccc ttcagccgcc gggtctgcgc gcagacgtac ccgctgtcaa tctggatttt
     4981 cgctcccagg gcgcgaagtc ctttgagatg gagatcgatc gggcgggtgc cgatgacgca
     5041 tccgccaggc agtgagactt tggcttgtcc caagcgcccc agcagcgggc ctagaaggca
     5101 gacggagccg cgcatcttgc gcaccaggtc gtagtcgcag aagccgcgga tgcgattggc
     5161 ctcgatcgag acggtgtcgc cctgggaccg gactttggcg cccaccgcgg tcaggatgcg
     5221 aaccatgaac gccacgtcgc tcaacgccgg cacgcgccga atcacgcacg gctcacgggt
     5281 cagcagcgcc gccgccatca gcggcaacac agcattcttg gccccgctga ttgtgacctc
     5341 gccttccagc ggtcgcccgc cctcgatcaa cagactgtac atggcacgca acttgttccc
     5401 cgcgccgcat cgagacggcg cgccccgtcc gtctccccga cacaccggcc gcggctggac
     5461 tgtgggcggc aatgtttcat cttcgggcag gccgcgcaat caagattcgt tcccgcccgc
     5521 tcagatcggc ctcgacggcc tcgacgatcc agccgtgtcg aacgaagagg tctgaaacgg
     5581 ctgtggcctg gccatcaccg aattcgacca gaagatggcc ggatcggttc aggcgagccg
     5641 cagccccggc ggccaattgg cggatcacgt ccagaccgtc tggacctccg tcgagtgccg
     5701 ggcgcggatc gtgatcgcgg acctccgggg cgagacgctc gatctcgacg ctcgggatgt
     5761 agggaggatt ggcgacaatg acgtcgaagt gcagccccgg gcgaaccgcg gccaggccgt
     5821 cgccgcgcgc ccattcaatg cggtcggcga ctccttgtcg ttccgcgttc gcgcgggccc
     5881 ggaccagggc cggttcggag atatcgaggg cggtgatgtg cgcggcgggg caatgccggg
     5941 ccagagcgat ggccaggcaa ccgctgccgg tcccgaagtc caagaccgcc ggcgcggcga
     6001 ccggcaaagt ctcgagaagt gtccaggcgc gctcggccag gacctcggtt tccgggcgcg
     6061 ggatcaggac atcagggccg acagcgagtt cgaacccgca gaacgacacc aggccggtca
     6121 ggtgctgcaa gggctcgcgg ttgccgcggc ggcggaccag tgcgcggacc gttgccagat
     6181 cggattcgcc gagggtgcgc tcgaagttca ggtagagctg gagccggggc agcttcagca
     6241 catgggccag gagcaactcg atttgcagcc ggggcgagtc cacccccttc tgagccaggt
     6301 agtcggtgct gcgtcgaatg acttccaaga ccgtcacaag acaagagtaa cgccgccggt
     6361 cggcggggtc agccacgaac tatgcacaac gtcagaagtg acaccgcgtg ggggcatgcg
     6421 gccaacaacg ccctgtaggc cggcgcctct gaccgggcgt cattgactta tgacgctgcc
     6481 tatcgaaatg ctgatggccg ttgacggcgg ggccttggcg tgatagcccc tgtgctcgga
     6541 taatgttgga gtctttttcg ccagtcccaa accgatgcaa gacctgatct cgaaagccgc
     6601 gacactgctc gaggctttgc cctatctgca gaagtacagc ggggccacgt ttgtcgtcaa
     6661 atacggcggc agcttcatgg actcgcccga cccggtcatc cggaccagcg ttgcgcggga
     6721 cattgttttt ctcgaggctg cggagatcaa tcccgtcgtg gtgcatgggg gcggcaaggc
     6781 gatcacgcgc gccatggaaa aagcggggct gaaggcgcaa ttcgtccagg gccagcgggt
     6841 tacggacgag gcgacggtgc gggtggtcga tgcggtgctt tcgggcgaga tcaatccgga
     6901 ggtggtgaca acgatcagct cgttaggcgg gctggccagg ggtttcgccg gcacggaggt
     6961 tttcacctgt cggaagcttc tggtgcacgg gccggacggg cagccgctgg acatcggttt
     7021 cgtgggcgaa gtgaccggcg tgaacacagc cccgctcctg gcctgcatcg agaaggggat
     7081 cacgcccgtg atcagcccca ccgcgcggga caaggacggc caggtgtaca actgcaatgc
     7141 ggacgtcgcg gcggcgcgcg cggccatcgc gttgcgcgcg cggcggctgg ttttcatgag
     7201 cgatgtgccg ggcttgctgc gcgaccccaa ggaccccgcc acggtgattc cgcatctgcg
     7261 cgtcagcgag gtggacgctc tcaagcacgc cgggattgtc gagcggggca tgattcccaa
     7321 agtcgacagc gcggtcgccg ccattcgggc cggggtcgac aaggtctcgt tcgtcgacgg
     7381 acgcgtgcct cacgcggtgc ttctcgagat attcaccgac gaaggcgttg ggacggaggt
     7441 ggtgctctag ccccatattg cgcaaatccg ggctggcccg ccggacgccg cttggatcgg
     7501 acggactccc ttttatgaaa gacatcattc cgcctccacc catcatcgcc aatcagttcg
     7561 agacggtgcg cgagcttttc cagaagtacg tcgtgccgag ctacggccgc ttcgacctcg
     7621 cgttcgcccg cggcgccggc agtttccttt gcgatgtgtc cgggaagcgc tacctcgacc
     7681 tgggcagcgg gattgccgtc tgctcgctgg gccatgcgca tccggaaatc actgaggccc
     7741 tgatcgagca gtcgcggaag atggtgcaca tctcgaatct ctattaccac gagccccagg
     7801 ggcgccttgc ggaacagatc gtacgcctgc tggcgcccgg caaggtgttc ttcgccaact
     7861 cgggtggcga ggccaacgag ggcctcttca agctggcccg caagttcgga cacgacgagg
     7921 gccggttcga gattctcacc gcgctgaatt cctttcacgg ccggacgctg gcaggtatcg
     7981 cggccaccgg tcaggacaag gtcaagagag gattcgagcc gatggtggcc gggttccgcc
     8041 acgtcccctt caacgacctc gaggcaatgc gccaggcgct ttccccggcg acggtggcca
     8101 tcctgatcga gggcgtccag ggcgaaggcg gcatcacccc cgccagcccc gagtacctgc
     8161 ttggactccg cgccctgtgc gacgaaaaga gactcctctt gcttatggac ggcgtccagt
     8221 gcggttattt ccgcaccggc cggttccaga gtttccagcg cattctcgaa ggggtgtccg
     8281 gcgccgacgg ttttctgccc gctggggtgt cgatggcg
//
LOCUS       DAOQRO010000129         8287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8165_length_8287_cov_4.966715, whole genome shotgun sequence.
ACCESSION   DAOQRO010000129 DAOQRO010000000
VERSION     DAOQRO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8165_length_8287_cov_4.966715"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..960
                     /locus_tag="PKK20_06440"
     CDS             214..960
                     /locus_tag="PKK20_06440"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNU99558.1"
                     /translation="MNLTLSIVFAFAPGVVWCAEQTPAEVEGRLLAAELRNQRPEEPL
                     SSSGVLRTRDPYGKWREAIPVRLEIAPGAQYWQTRYWALGASQEPFETLTVTHAEGAP
                     TRYDYSRALGTAAVSDTVTLTGDAAAIPFAASEFWLSDLGLEFLDWPGQRVVRSEMRK
                     GRSCKVLESLNPNPSSTNYARVLSWIDIEHRGILRAEGYDQKRRLLKEFSIGSFKKVQ
                     GRWQLKSMEIRNEETDARTRIEFDLEIEDR"
     gene            complement(1167..2276)
                     /locus_tag="PKK20_06445"
     CDS             complement(1167..2276)
                     /locus_tag="PKK20_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNU99559.1"
                     /translation="MNSIVLLAPLLLAALTLDAAETPASRPRAMPVVSPEVQSGRRVT
                     FRLQAPRAEEVVVMGQWPDGRTPMTKDAQGVWSAEVGPIDAGVWEYSFQVDGLAMIDP
                     GNPAIKPMRQPRTSILHLPGDPPLLHDFRDVPHGALHQHTYVSKTLGRPRSMVVYTPP
                     GYGLEPDRRYPVLYLQHGMGDNEATWTAHGKAHWILDNLIAQGRARPMTIVMLDGHAA
                     IGGPTAFGGNSAAFELDLIDDAMPFIEANYRVAAEAKSRAIVGLSMGGEQALRIGLTR
                     PDRFAWIGGFSAATPMRDAVKSALDDAASINAQVKLLWIACGRDDFLLRRNEDFIAVL
                     KEKGIRHEWLLTEGNHSWPVWRGYLSQIAPRLFQP"
     gene            complement(2461..3873)
                     /locus_tag="PKK20_06450"
     CDS             complement(2461..3873)
                     /locus_tag="PKK20_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99560.1"
                     /translation="MNRPPIALTSRRDFLKNTGRFAAASALAGVALPRVHPAGSDTIQ
                     VALIGCGGRGTGAAANALAVKHGSLKLVAMADVFAERLDGSYQALRQAATAPIEGAPE
                     TRVGGFAASQIEVPPDRRFLGFDAYQKAMDCLKPGDIAILTTPVAFRGIHFEHAIRKG
                     LHVFMEKPITTDGPTTRRMLALGEESVKKNLKVGVGLMCRHCQARWELYRRIKEGEIG
                     DITTLRTYRLVAPAGFTGPKPEGMSEVLYQVQRYLGFFWASGGVFHDYVAHNVDECCW
                     MKEAWPVQAQGMGARLYRGDAVDQNFDHYSIEYTFEDGTKLFLQSRYMPGCHEEFASY
                     AHGTRGAAVISTFVHTPAKCRVYRGHAMARENLAWAFPQPEPDPYQLEWDHLVEAIQQ
                     DQPFNEVKRGAEASLVVLMGRRAAHTGQIVTFDDMLQCDEEFAPGLEQLTADSPAPVR
                     PGPDGLYPSPQPGLKKTREC"
     gene            3994..4653
                     /locus_tag="PKK20_06455"
     CDS             3994..4653
                     /locus_tag="PKK20_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99561.1"
                     /translation="MKTRSGFTLIELLVAAGILAILATLLLPALIRAKSRVQAIECAN
                     NLRQWGLATQLYAADHDDYLPPDGSPNPTRTATNVGWYIQLPAQLGLPRYHDQAWRTN
                     PLAAPAKSIWTCPANKRRSNGRNLFHYCLNEHVNGTGDEARPIRLGAIAEPSAVIWLF
                     DTKNLPAVGGWSFVPTNLHNAGAQFVFLDGHVVRHRCTAYWDFERGKARTNHPSLRWW
                     P"
     gene            5019..8222
                     /gene="carB"
                     /locus_tag="PKK20_06460"
     CDS             5019..8222
                     /gene="carB"
                     /locus_tag="PKK20_06460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNU99562.1"
                     /translation="MPKRTDIHKVLIIGSGPIVIGQACEFDYSGTQACKALRSLGYKI
                     ILVNSNPATIMTDSGTADVTYIEPLTLAALTEIIEKERPDALLPNLGGQTGLNLSAQL
                     AKAGVLARYGVQIIGVEADAIEKGEDRIIFKETMKRLGIEMPRSAPAFSVEEAERVAA
                     ELGYPVVVRPAYTLGGTGGGHVYNVEELRLVANRGLAASMVGQILIEESVLGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSLCVAPMLTIDPKLQARLQEYSYRIVESIG
                     VIGGTNVQLAHDPRTGRVVVIEINPRTSRSSALASKATGFPIALISAKLASGLTLDEI
                     PYWRDGTLEKYTPSGDHVVVKFARWAFEKFKGAEDKLGTQMRAVGEVMSIGKNYKEAF
                     QKAVRSLENGRYGLGFAKDFNGKSLPELMALLNEPTSERQWLMYEALRKGATVDELSA
                     KTHIKAWFIQQMKELVDLEERILAHRGRPLPDALLIQAKRDGFADRYLAQLLDVPEAD
                     LRERRIALGVVECWDAIPVSGVEKAAYYYSTYNGPDRVAVSTRKKVMVLGGGPNRIGQ
                     GIEFDYCCVHAALALRDAGYETIMVNCNPETVSTDYDTSDRLYFEPLTTEDVLAIYQK
                     EQPEGMVVQFGGQTPLNIAGELAAAGVRILGTSPDTIDLAEDRDRFRQMMERMGIPMP
                     ESGMASTFEEARAIAQRIGYPVMVRPSFVLGGRGMEVVHDEEMLRRYVAAAVDVTPKR
                     RILIDRFLANAIEAEADALADGTDAFVPSVMEHIELAGVHSGDSACAIPAVSLPEQHL
                     RTIEEFTLRIGRELHVCGVMNIQYAICDGRVYVLEANPRASRTVPLVSKVCNVPMARI
                     ALQLMLGARLRDLRLERRTIPHVGVKEVVLPFNMFPEVDPVLGPEMRSTGEVLGLAPT
                     FGLAYFKSQEAATPPLPLQGTVFISVTDADKPALADVAKHFLRLGFRVKATEGTQAFL
                     ERQGIASERSFKVREGKRPNVVDEIKSGAIALVINTPIGKTSQFDDAYIRKAAIKYKV
                     PYLTTVAAARAAVVGIEASRSGHAGVKSLQSYHADIG"
BASE COUNT         1527 a         2641 c         2658 g         1461 t
ORIGIN      
        1 acgttccccg gttcatgggt tcaatgcgca ttaaaccgtt gaatgtcaat gcttttcatg
       61 aatcacgctt tacttcggag ggccgctccc gcggctgcgg cagaggccat gatcggtgat
      121 gatgattgtc tctatccagg gactcgcgga gttcgtcgct ccgagaaggg ctcatgttca
      181 ctgcgcactt caacacggaa cagactgctg ttcatgaacc tgactctctc gatcgtgttt
      241 gcattcgccc cgggggttgt gtggtgtgcg gaacagacgc ccgcagaggt cgagggccgg
      301 ctcttggcgg ccgaactgcg gaatcaacgc ccggaagaac ctctcagcag cagcggcgtt
      361 ctgcggaccc gggatcctta cggcaaatgg cgggaggcga ttcccgtgcg gctggagatc
      421 gcgcccggcg cgcagtactg gcagacgcgg tattgggccc tgggcgccag ccaggagccg
      481 ttcgagactc tgactgtgac ccatgcggag ggggcgccca cgcgctacga ctactcgcgc
      541 gcgctcggga cggccgcggt ctcggatacg gtcaccttaa ccggcgacgc ggcggccatt
      601 ccgttcgcag catccgagtt ctggctgtcc gatctggggc tcgagtttct cgattggccg
      661 gggcaacggg tggtgcggag tgagatgcgc aagggacgat cgtgcaaggt cctcgaaagt
      721 ctgaatccaa atccgagttc aaccaactac gcgcgcgtgc tgtcgtggat cgacatcgag
      781 catcggggca ttcttcgtgc cgagggctac gatcagaagc gccggctcct gaaggagttt
      841 tcgatcggca gcttcaagaa ggtccagggc cgctggcaat tgaagtcgat ggagattcgc
      901 aacgaagaga cggacgcccg gacgcggatc gagttcgatc tcgagatcga ggatcgttag
      961 cgaggcttcg catcagacca aggaggcgtg cgaacaactc gtaaccaccg ggtgacaacc
     1021 acggtcaccg acgcccgatg catcgactcg actcatttgc cgcagatttc acctggggag
     1081 caactccagt gaagctgcct aacccacatg gcaatcatct tgcgaagtct gaccgccatg
     1141 tgcactgacg gagtgaaata ctcgagtcag ggctggaaga ggcgcggggc gatctggctc
     1201 agataccccc gccaaaccgg ccagctatgg ttcccttcgg tcaacagcca ttcgtgtcgg
     1261 attcccttct ccttgagcac ggcaatgaag tcttcgttcc gcctgagcag aaaatcgtcc
     1321 ctgccgcagg caatccagag cagcttcact tgggcattga tggacgcggc atcgtcgagc
     1381 gcggatttca cggcgtcgcg catcggcgtg gcggcgctga acccgccgat ccaggcgaaa
     1441 cgatcggggc gagtgagccc aatgcggagg gcctgctcgc ctcccatgct caggccgaca
     1501 atcgcgcggg attttgcctc cgcggccacg cggtagttgg cttcgatgaa gggcatggca
     1561 tcatcgatca gatcgagctc gaaggcggcc gagttgccgc cgaaggccgt cggacctccg
     1621 attgcggcgt ggccatcgag catcacgatc gtcatgggtc gggcgcgacc ttgagcaatc
     1681 aggttgtcga gaatccaatg cgccttgccg tgggctgtcc aggtggcctc gttgtctccc
     1741 atcccatgct gaaggtagag gaccgggtac cgtcgatcgg gctcgaggcc gtatccgggc
     1801 ggcgtgtaga cgaccatcga gcgcgggcgg cccagggtct ttgagacata cgtgtgctga
     1861 tgcagcgcgc cgtgggggac atctctgaaa tcgtggagga gcgggggatc gccgggcaga
     1921 tgcagaatgc tagtgcgggg ttggcgcatc ggtttgatgg ccggattccc agggtcgatc
     1981 atcgccaacc cgtcgacctg gaagctgtat tcccacaccc cggcgtcgat cgggccaacc
     2041 tcggcgctcc agacaccttg cgcgtccttt gtcatcggcg tccgcccgtc aggccactgg
     2101 cccatgacga cgacctcctc cgctcgaggc gcctgcaagc ggaacgtaac acgccggccc
     2161 gattgcacct cgggagacac gacgggcatg gcgcgcggac gcgacgccgg tgtctcggcg
     2221 gcatcgagcg tgagagcggc aagcagcagg ggcgccagca gaacgatgga attcataagc
     2281 gaatccttta cgctccttgg acgcgcgtgt caaacccggc gctcttccca gacgaaatcc
     2341 gtggcaaatg agtcaagtct tggcgctgcg cagcggcgcc acgggtgccg gtgatcgcgg
     2401 ttgccacccg gcggccacga attgttcaca cgcccctcct tggtctgaag cgaagcctcg
     2461 ctaacattcg cgcgtcttct tgaggcccgg ctggggagac ggatagaggc catccggccc
     2521 cggtcgaacc ggcgcgggcg aatcggcagt caactgttcg aggcctggcg cgaactcctc
     2581 gtcgcactgg agcatgtcgt cgaaggtgac gatctgaccg gtgtgagcgg cgcgacggcc
     2641 catgaggacc accaggctgg cttcggcgcc gcgtttcacc tcgttgaacg gctggtcctg
     2701 ctgaatggct tcaaccaggt gatcccattc gagctggtag ggatccggct cgggttgggg
     2761 aaacgcccag gccagattct cgcgggccat ggcgtgtccc ctgtaaaccc ggcacttggc
     2821 gggggtgtgg acgaacgtcg agatgacggc ggcgccgcgt gtgccgtggg cgtaactggc
     2881 gaattcctcg tggcagccgg gcatgtagcg tgactggagg aaaagcttgg tcccatcctc
     2941 gaacgtgtac tcgatcgagt agtgatcgaa gttctggtcc acggcatccc cgcggtagag
     3001 acgggcgccc atgccctggg cttgaaccgg ccaggcctcc ttcatccagc agcattcgtc
     3061 gacattgtgg gcgacgtagt cgtggaacac gccgccgctg gcccagaaga agccgagata
     3121 ccgctgcacc tggtagagga cctcgctcat gccctcgggc ttgggccccg tgaaaccggc
     3181 cggcgcaacc aaccggtaag tccgcaacgt cgtgatgtcg ccgatttcgc cctccttgat
     3241 ccgcctgtac aactcccagc gggcctggca gtgacggcac atcagcccga cgccgacctt
     3301 gaggttcttt ttcaccgact cttcgcccag tgccagcatc cggcgcgtgg tcggaccgtc
     3361 agtcgtgatc ggtttctcca tgaacacatg cagccccttg cgaatcgcgt gctcgaagtg
     3421 gatcccgcga aaggccactg gtgtggtgag aatcgcgatg tcgcccggct tcaggcagtc
     3481 catcgccttc tgataagcgt cgaacccgag aaaacgccgg tcaggcggca cttcgatttg
     3541 ggacgccgcg aagccgccga ctcgggtctc gggcgcaccc tcgatcgggg cggtggcggc
     3601 ctgacgcagg gcctggtaac tgccgtccag acgctcggca aagacatccg ccatcgccac
     3661 cagcttcagc gagccgtgct tcaccgccag agcgttggcc gcggcccccg tgccgcgtcc
     3721 gccgcaaccg atcaaggcaa cttgaatcgt gtcgctgccc gccggatgga cgcgcggcaa
     3781 ggccacgccg gccaaggcgg acgcggccgc gaaacggccc gtgttcttca ggaagtcccg
     3841 acgagatgtc agcgcgatcg gcggtcgatt catggcgcgg atagtgtgca tcgggccggc
     3901 gcaggtcaat gcggcattgg cgacggcgga cattccttgc gccccggcgt cggatgcgta
     3961 cattaatgct cgtcaaggag aggaggtagt cccatgaaaa cacggtctgg cttcacgttg
     4021 atcgagcttt tggtggcggc gggcattctg gcaattctgg caacgttgct cctgcccgcc
     4081 ctgattcggg ccaagtcccg cgtccaggca atcgagtgtg cgaacaacct gcgccaatgg
     4141 ggcctggcga cccagctcta cgcggcggac cacgatgact atctgccgcc tgacggctcc
     4201 cccaacccca ctcgaacagc gacgaacgtc ggatggtaca tccagttgcc cgcccagttg
     4261 ggccttccga ggtaccacga tcaggcctgg cgaacgaatc ctctggcggc tccggccaag
     4321 tcgatctgga catgcccggc caacaaacgg cgcagcaacg ggcgcaacct cttccactac
     4381 tgtctgaatg aacacgtcaa cggcaccggc gacgaggcgc gtccgatccg gcttggcgca
     4441 atcgctgaac cctccgctgt catctggctg ttcgacacca agaacctgcc ggccgtcggg
     4501 ggttggtcgt ttgtcccgac caacctgcac aatgcagggg cgcagtttgt gtttctcgat
     4561 ggccacgtcg tgcgccaccg gtgcacggcc tactgggatt tcgagcgcgg caaggcccgc
     4621 accaatcacc ccagcctgcg ttggtggccc tgacgccgcc catccttcgc tcaggattcg
     4681 gtgcatctgg ttgttgtcgg tgcttatgcc gcccgatgat ggcactgggt ctacatcggt
     4741 tgcagactcc cggtgtaagc cgtgtgccct gcctgcgccg caaagcgccg cggcctgtct
     4801 gccgtgtcgg acacgccgca agcaggcgcg gcaggcaggc cccagacggc gcaaactcgc
     4861 tccccaccga cactctccag atgctcggaa ttgagcgtcg ggatttgagt tgcgggggct
     4921 gcgcggctgc cgctataagt cacgccgctt tttgatgcgc cggaacccgg aaaccgcctc
     4981 cgggcctctg caaccgttgt accctaacga gtgcccttat gcctaaacga accgacatcc
     5041 acaaagtcct catcatcggc tccggtccca ttgtgatcgg gcaggcctgc gaattcgatt
     5101 attccggcac gcaggcgtgc aaggcgctgc gcagcctggg ctacaagatc atcctcgtga
     5161 actcgaatcc ggccaccatc atgaccgatt cgggcacggc ggacgtcacc tacatcgagc
     5221 cgctcacgct cgcagctctg accgagatca tcgagaagga acgacccgat gccttgctgc
     5281 caaacctggg gggccagacc ggattgaacc tctcggcgca actggccaag gcgggcgtgc
     5341 tggcccgtta cggcgttcag atcatcgggg tggaggcgga tgcgatcgag aagggcgagg
     5401 accggatcat cttcaaggag acgatgaaac gcctgggcat cgagatgccg cgcagcgcgc
     5461 cggcgttcag cgtcgaggag gcggagcgtg tggcggcgga gctgggctac ccggtcgtgg
     5521 tccgtccggc ttacacgctg gggggcacgg gcggcgggca cgtctacaat gtcgaggaat
     5581 tgcgtctggt ggccaatcgc gggctggcgg ccagcatggt gggacagatt ctgatcgagg
     5641 aatcggtgct cgggtgggag gaactcgaac tcgaggtggt gcgcgacgcc aagaaccaga
     5701 tgatcaccgt ctgcttcatc gagaacgtgg atgcgatggg cgtgcatacg ggcgattccc
     5761 tgtgcgtggc gccgatgctt acaatcgacc ccaaactgca ggcccgcctg caggagtact
     5821 cctaccgcat cgtcgagtcg atcggcgtga tcggcggcac caacgtccag ttggcccacg
     5881 acccgcggac cggacgggtc gtcgtcatcg agatcaaccc gcgcacctcc cgatcgtccg
     5941 cgctcgcgtc gaaggcgacc ggcttcccga tcgcgctgat ctcggccaag ctcgccagcg
     6001 ggctgacgct cgacgaaatc ccatactggc gggacggcac gctcgagaag tacacgccct
     6061 cgggcgacca tgtggtggtg aagtttgccc gctgggcttt cgagaagttc aagggggccg
     6121 aggacaagct cgggacccaa atgcgtgccg tcggcgaggt catgagcatc ggcaagaact
     6181 acaaggaagc gtttcagaag gccgtccgct cgctcgaaaa cgggcggtac ggactcggct
     6241 ttgccaagga ttttaacggg aaatcgctgc ccgagctgat ggcgcttctg aacgagccga
     6301 ccagcgagcg ccagtggctg atgtacgagg cgctccgcaa gggcgccacg gtggacgaac
     6361 tctccgccaa gacccacatc aaagcctggt tcatccaaca gatgaaggag ttggtcgatc
     6421 tcgaagagag gattctcgcg catcgcggac gtccgctccc cgacgccctt ctcatccagg
     6481 ccaagcgcga cggcttcgct gacaggtatc tggcccagtt gctggacgtg cccgaggccg
     6541 atctgcggga acggcgcatc gccctcggcg tggtcgagtg ctgggatgcc atcccagtca
     6601 gcggtgtcga gaaggccgct tactactact cgacctacaa cggccccgac cgggtcgccg
     6661 tcagcactcg caagaaggtc atggtccttg ggggtggacc caaccgcatc gggcagggga
     6721 tcgagttcga ttactgctgc gtgcacgcgg ccctggccct gcgcgacgcc ggctacgaaa
     6781 cgatcatggt caactgcaat ccggagacgg tgtcgaccga ctacgacacg tccgacaggc
     6841 tttatttcga gccgctgacc accgaggatg tattggcgat ctatcagaag gaacaaccgg
     6901 agggcatggt cgtccagttc ggcggacaaa ccccgctcaa tatcgccggc gaactcgccg
     6961 cggccggcgt gcgcatcctg ggcacgtcgc ccgacaccat cgatctcgcg gaggatcggg
     7021 accgcttccg ccagatgatg gagcggatgg gcatcccgat gcccgagtcg ggcatggcca
     7081 gcacgttcga ggaggcgcgc gcgatcgccc aacgcatcgg ctatcctgtc atggtccggc
     7141 cttcgttcgt cctgggcgga cgcggcatgg aagtcgtcca cgacgaggag atgctccggc
     7201 gctatgtggc cgcggcggtg gatgtcacac ccaaacgccg catcctcatc gaccggtttc
     7261 tggccaatgc catcgaggcc gaggccgatg cgctggcgga tggcacagac gccttcgtgc
     7321 cgtcggtgat ggaacatatc gagctggcgg gcgtgcactc gggcgattcc gcctgcgcca
     7381 tcccggcggt cagcctgccc gagcaacacc tccgcaccat cgaggagttc acactgcgca
     7441 tcgggcgtga actccacgtc tgcggggtga tgaatatcca gtacgccatc tgcgacggcc
     7501 gggtgtacgt ccttgaagcc aatccccgcg cctcccgcac cgtcccgctg gtttcgaagg
     7561 tctgcaacgt gcccatggcc cgcatcgcgc tccagctcat gctgggcgcc cggctgcgcg
     7621 atctgcgcct cgaacgccgc accatcccgc acgtcggcgt taaggaagtt gtgctgccct
     7681 tcaacatgtt tcccgaagtc gaccccgtgc tcgggccgga gatgcgatcg acgggagaag
     7741 tcctcggcct ggcgcccacg ttcggcctcg cctacttcaa gagccaggaa gcggccacgc
     7801 ccccgctgcc gctccagggc acggtgttca tttcggtgac cgatgcggac aagcccgcgc
     7861 tcgcggacgt ggccaagcat ttcctgcggc tgggcttccg ggtcaaggcc accgagggca
     7921 cacaggcctt cctcgaacgg cagggcatcg cctccgaacg cagcttcaag gtccgcgagg
     7981 gcaaacgccc caacgtggtg gacgagatca agagcggggc gatcgctctg gtcatcaaca
     8041 cccccatcgg caagaccagc cagtttgacg atgcctatat tcgcaaggcg gccatcaagt
     8101 acaaggtccc ctacctcacc accgtggcag ccgcccgcgc ggcggtcgtc ggcatcgaag
     8161 ccagccgatc gggccacgcc ggggtgaaat cactccaaag ctaccacgcc gacatcggct
     8221 aagggtccgt gcaaaattac ttccgatttt ggcgggagcg ccaccgggcc agatgcaagg
     8281 cccgagg
//
LOCUS       DAOQRO010000130         8263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8191_length_8263_cov_4.697734, whole genome shotgun sequence.
ACCESSION   DAOQRO010000130 DAOQRO010000000
VERSION     DAOQRO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_8263_cov_4.697734"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3266
                     /locus_tag="PKK20_06465"
     CDS             <1..3266
                     /locus_tag="PKK20_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNU99563.1"
                     /translation="SVWWKGRAVVDNLAIDFNPSPGRLVFGGRTGDAYQNTHVDDIVL
                     TTIPADKPLITTASADAISVSIQLEDAGAVTVKQDTVKMKVNGADATPLTFTKTGTTT
                     TARWASPTYLASGSTVTVDVEFMDSSDRTITGTRTAVVAAYNHLPAAYQVPTESVDTG
                     KPGFLIRPYQTEALQPNTLWWTEEQLAGLRGDNIADLTGATGGFYTHEGVINFNVAAP
                     AAVGNFNVDNGFEDMMFPGIPGTTGLTGSSTEEMLAYIEFPTAGMYQLGVNSDDGFRV
                     SVGTAPGDLTGLILGQYDGGRGASDTIFTIVVDPPGIYPIRLVWENGAGELPGNGANC
                     EFFSVKDGVKTLINDVANGGLKAYRESSATAPWISKIRPLRGEAVLPDPVITAEITHG
                     ASPVNDGSVKVKLNGAETAADVVSAGGKTTVTIPRPADFLPAGTYTVTIDLTDSATPA
                     KAYTFQWQFTVGDYQVYQAPYGAGGTWNVYLVTRGAKNWVAAEMAAKASVEPFSGQKK
                     PGHLVTLHSAEERDFVRMIATGESVWIGLTDNELYGGFESGTDGSVGWVWVTGEEYTW
                     YEWNAGEPNDYPPGEDAAELIGSGLFNDNSSGIPPEGSGTTRFYVVEYETRASAKLAN
                     IRPGLFPDGPLPGPEASCGAFAIRAVRDVGNVGNIATAFNALYNANASIVDTTSPVVN
                     FNDPGGAGGPGIFRAELTFPGDRDGDDNDFVISATTKIRITEAGLYTFGVHSDDGFAL
                     RIRGKNWVSVSGNGQFAPGGTDTITYQFGTGDSNTRGVIDLEVGEYDLDFVFFEDGGG
                     AFVELYAAKGEYANDADAEKWQLVGVNNLLVPGVSDAGWTVAYSQPGGTALNSIADAE
                     AELVGAPTVTGVPKIQYADPDAAGGTQAGYLPFPNDTPADDNDFAILGEAQLVIPADG
                     TYYFGFQGDDGGYLQIVGQTWDRIVATANASLGIINGDRIQFDANTGDSYTVGAITLA
                     QGTYTIRTLFWERGGGAYHWVFGSAPNDVHFPGSVLSANAATVGTWPLEIVPCAGKPE
                     ITVTKNTDGTITISWTNGGTLQAATSILGPWQDVTDTSPYTFTPQADVPYMFGRVKK"
     gene            3418..3912
                     /locus_tag="PKK20_06470"
     CDS             3418..3912
                     /locus_tag="PKK20_06470"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNU99564.1"
                     /translation="MAKLVVLTEGFAGTSFELKGERTTVGRMEDNAFQIAEPSISSHH
                     CEILVRGHEVVVRDLNSTNGTFINGQPIKEAVLKPGQIMRLGAVDIRLDDGTPPPPAK
                     RQQDHTQLLPGGVKLTELEQGGRQVGSPFAKKSAGANTLFLYVGIALVVLIIGFIVFL
                     VVQL"
     gene            complement(3919..4935)
                     /gene="hemB"
                     /locus_tag="PKK20_06475"
     CDS             complement(3919..4935)
                     /gene="hemB"
                     /locus_tag="PKK20_06475"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNU99565.1"
                     /translation="MNLPSLAQFPVYRPRRLRQSAALRRLAQETTLDVQRLVLPLFVR
                     PGRRVRNPIGSMPGVFQLSADELVKEAAAAHELGVPAVLLFGIPARKDARASEAYASK
                     GIVQSTVRLLKRELPSLLVITDVCLCEYMSHGHCGLVETAGRHAGRIRNDPTLALLAR
                     AAVSHADAGADIVAPSDMMDGRVRAIRAALDGAGFADTPIMAYAAKYASAFYGPFREA
                     AESAPKFGDRRSYQMNPANGLEALREVALDIQEGADIVMVKPALAYLDVLYRVKTTFG
                     CPTAAYAVSGEYAMIKAAAANGWLAEKAVTIESLLALRRAGADILITYSASDLARWHR
                     DGDI"
     gene            complement(4975..7014)
                     /locus_tag="PKK20_06480"
     CDS             complement(4975..7014)
                     /locus_tag="PKK20_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99566.1"
                     /translation="MKTSCVFVLAALCGGVATSCLAQTGPFDPDQWPATVDSSKVVHY
                     VVTDGGLEPPDVYWVENLNILTGGDQVTEDFSIGGHTGKKVTGSFLNVADADYWQWAD
                     EEFIDILVQVYGDAALFNAQGQPRDFNFLIGTLPPTYLSAPVGGQIPIDAKNKRWNWV
                     LFRIPNGIRPDGERFCGSLSAAAQGNTGAGGVNGGTIRMESVPGLIVRVIAFGQEGAF
                     GPPEDINKFLPAETCDPEPETNLAGIDVAAATSNHMQVLNDGDQTVTYAEDVGPAGDK
                     RRAVVPDGLYLNFGITDNYLGLPCNDPRTVKVCVDFYDDPAFAGLGVVFGPEAYATDA
                     AGGIGFVAAANRHTMEGSGVWIRRSWTVPAVNLKGVNAGALTAGPRFISENGQVAVSR
                     FELAILRVGDHPLAGQDPLETCYEDPNICTDKYGSFVELDLAQGIKNGLDVGNSGGDQ
                     EMIVAEAGPAGDIRMAVRPAREDGSPGFTHQYLNFAILDQALGPNSQPPARLAICVTY
                     YDDPALVGANFKPDVYQTVRNGVLTLAFPDNSYLVTLEGTDTWRTAYWEIPDMKFNGV
                     NQGPQAAARFTVSDKVFFTSVKYAVIRPCGPNAGVNLLESCKPVLEVPLAIERVGTNV
                     TLSWPAAATGFAVQGTSSLAAPDWAVVNATVEVSGDRNVVTLPTSGATMFLRLVK"
     gene            complement(7116..7913)
                     /locus_tag="PKK20_06485"
     CDS             complement(7116..7913)
                     /locus_tag="PKK20_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU99567.1"
                     /translation="MKTIIHWPRRRNGVAGGGFTLIELLVVIAIIAILAGMLLPALSR
                     AKEKGRSISCVNNLRQMLVASKVYADDNQSRFPWTFSLIGNQLNRKAWFNYLQPYQQT
                     TNLLRCPTRTQKFKEFLAVYPTDLADQAVSNYGANFRLGGCDWPGVWDKAQWPAIRDT
                     DVKCPSGVVQITDSATQPLRTRDPLQCVTPASKEKPGAWIVHDPEADEPCTGCVTSPG
                     DPNWGGPHLRHNGRSNVAFVDAHAESLKAQTWYWGGTPWLKPNEGGN"
BASE COUNT         1609 a         2715 c         2489 g         1450 t
ORIGIN      
        1 tgtcggtgtg gtggaagggc cgcgcggtcg tggacaacct ggcgattgat ttcaatccga
       61 gcccgggccg tctggtcttc ggcggtcgca cgggcgacgc ctaccagaac acgcatgtcg
      121 acgacattgt cttgaccaca atcccagcgg acaaaccgct gatcaccacg gccagcgctg
      181 acgccatcag cgtctcgatc caactcgagg atgccggcgc tgtcaccgtg aaacaggaca
      241 ccgtgaaaat gaaggtgaat ggtgctgacg caacgccact gacattcacc aagaccggca
      301 caacgaccac ggctcggtgg gcctcgccca cctacctggc ctcgggctcg accgtgaccg
      361 ttgacgtcga gttcatggac agttcggatc ggaccatcac gggcacgcgc acggcggttg
      421 tcgcagcgta caaccacctg cccgccgcct accaggtgcc gaccgaatcg gtcgacaccg
      481 gcaaaccggg cttcctcatc cgcccgtatc agaccgaagc cctccagccc aacaccctct
      541 ggtggacgga ggaacagttg gccggcctgc gtggagataa catcgcggat ctgaccggcg
      601 ccaccggcgg cttctacacg cacgaagggg tcatcaattt caacgtggcc gccccggccg
      661 ctgtcggcaa cttcaacgtt gataacggtt tcgaggatat gatgttcccc ggtatccccg
      721 gaaccacagg tcttaccggt tcctcgactg aagagatgct cgcctacatc gagttcccga
      781 ctgccggcat gtatcagctc ggcgtcaata gcgatgacgg cttccgcgtc agtgtgggaa
      841 ccgcccccgg cgacctgacc ggtctcatcc tcggccagta cgacggcggg cgcggggctt
      901 cggacacgat cttcacgatc gtggtcgatc ctcccggcat ctacccgatc cgcctggttt
      961 gggaaaacgg cgcgggcgag cttcccggca acggcgcgaa ctgcgagttc ttcagcgtca
     1021 aggacggtgt gaagaccctg atcaatgacg ttgcgaacgg cggcctcaag gcctaccgcg
     1081 agagcagcgc caccgccccg tggatctcga agatccgtcc actccgcggc gaggcggtcc
     1141 tcccggaccc ggtcatcacg gccgagatca cccacggcgc atcgccggtg aacgatggca
     1201 gcgtcaaggt gaagctcaac ggcgccgaga ccgcggccga tgttgtgagc gccggcggca
     1261 agacgaccgt cacgattccg cgcccggctg acttcctgcc tgctggcaca tacaccgtga
     1321 cgatcgatct gactgactcg gcgacgccgg ccaaggctta caccttccag tggcagttca
     1381 ccgttggcga ttaccaggtc taccaggcgc cttacggtgc gggtggcaca tggaacgtct
     1441 acctggtcac acgcggcgcg aagaattggg tggcggccga aatggcggcc aaagcctccg
     1501 tcgagccctt ctccgggcag aaaaaacccg gtcaccttgt caccctccac tccgccgagg
     1561 agcgcgactt cgtccgaatg atcgcaaccg gcgagagcgt ctggatcggc ctgaccgaca
     1621 acgaactcta cggcggtttc gagtctggaa cagacggcag cgtcggctgg gtgtgggtta
     1681 cgggtgaaga atacacctgg tacgagtgga atgcgggcga gcccaacgac tatccacccg
     1741 gcgaagacgc cgccgagttg atcggcagcg gcctcttcaa cgacaacagc tcgggtatcc
     1801 cgcccgaagg cagcggcacc acgcggttct acgtcgtcga gtacgaaacc cgggcatcgg
     1861 ccaaactggc aaacatcagg cccggcctgt tccctgacgg cccgcttcct ggacccgaag
     1921 cctcctgcgg cgcgttcgcc atccgagcgg ttcgtgacgt cggcaacgtc gggaacatcg
     1981 cgacggcctt caacgccttg tataacgcta acgcgtccat cgtcgacacc acctcgcccg
     2041 tcgtgaactt caacgacccg ggtggcgccg gcggccccgg catcttccgc gctgaactca
     2101 ccttcccggg cgatcgtgac ggggacgaca acgacttcgt catttcggca acgaccaaga
     2161 tcaggatcac cgaggctggc ctgtacacgt tcggcgtgca ctcggacgac ggcttcgccc
     2221 tgcgcatccg gggaaagaac tgggtgagcg tgagcggcaa cggccaattc gccccgggcg
     2281 gcacggacac catcacctac cagtttggca ccggtgactc gaatacgcgc ggcgtcatcg
     2341 acctcgaggt gggcgaatac gacctcgact tcgtgttctt cgaagacggc ggtggcgcgt
     2401 tcgttgagct gtatgcagcc aagggcgagt atgccaatga cgcggacgcc gagaagtggc
     2461 aattggtcgg cgtcaacaac ctgcttgtac cgggcgtgtc ggacgccggg tggaccgttg
     2521 cgtactcgca accgggcgga acggccctca actcgatcgc agacgccgag gccgaactgg
     2581 tgggtgcccc caccgtgacc ggcgtgccca agatccaata cgcggacccc gatgccgctg
     2641 gcggaaccca ggccggctac ctgccgttcc cgaacgacac gcccgccgac gataacgact
     2701 tcgccatact tggcgaggcg cagctcgtga tcccggccga cgggacctac tacttcggat
     2761 tccagggtga cgacggcggt tatctccaga tcgtcggcca gacctgggac cgcatcgtgg
     2821 cgaccgcgaa cgccagcctg ggcatcatca atggcgatcg cattcagttc gatgccaaca
     2881 cgggtgacag ctatacggtc ggagccatca ccctcgccca gggtacctac accatccgca
     2941 ccctcttctg ggagcgcggt ggcggcgctt atcactgggt gttcggatcg gcacccaacg
     3001 acgtccactt ccctggcagc gtcctctcgg ccaacgccgc aaccgtgggc acatggccgc
     3061 tcgagatcgt cccgtgcgcc ggcaagccgg agattaccgt caccaagaac accgacggca
     3121 cgatcaccat ctcgtggacc aacggcggaa ccctccaagc cgcgacgagc atcctcggac
     3181 cgtggcagga tgtgaccgac acaagcccgt acacgttcac gccgcaggct gacgtgccgt
     3241 acatgttcgg gcgcgtcaag aagtagcatt cactgcgctt ggacgcaatc gcatcgaggc
     3301 cggggcccac gccccggcct cttttcattt gccgacgatc gtaacgatcg aagctcgtac
     3361 agaaaccgtt tgacttggat cgccttgtcg gaataatcgg tccagacatt ccgacctatg
     3421 gccaagcttg tcgtcctcac agaagggttc gcgggcacct ctttcgagct gaaaggggaa
     3481 cggaccactg tcgggcggat ggaggacaac gcctttcaga tcgccgagcc atccatctcg
     3541 agccaccatt gcgagattct tgtgcgcggc catgaggtgg tggttaggga cctcaactcg
     3601 accaacggca cattcatcaa cggccagccc atcaaggagg ccgtcctcaa acccggccag
     3661 atcatgcgcc tcggggcggt cgatatccgc ctggacgacg gcacccctcc gcctcccgcc
     3721 aagagacagc aggaccatac acaactgctc ccgggcggag tcaaactgac cgaactcgaa
     3781 cagggcggac gccaggtcgg ctcgcccttc gccaaaaaga gcgccggggc gaacacgctg
     3841 ttcctctacg tgggcattgc ccttgtggtg ctgatcatcg gcttcatcgt gttcctcgtg
     3901 gtgcagcttt aggccgcatc agatgtcgcc gtcccggtgc caccgggcca aatccgacgc
     3961 cgagtaggtg atcaggatgt ccgcgcccgc gcgacgcagg gcgagcaggc tctcgatcgt
     4021 caccgccttc tccgcgagcc agccattggc cgccgccgct ttgatcatcg cgtactcgcc
     4081 gctgacggcg taggccgcgg tggggcatcc aaaggtcgtc ttcacgcggt agagcacatc
     4141 gagatacgcc aacgcgggct tcaccatcac gatgtcggca ccctcctgga tgtccagagc
     4201 tacctcgcgc aatgcctcga ggccgttggc tgggttcatc tggtaactgc gccggtcgcc
     4261 aaactttgga gccgattccg ccgcctcacg aaacgggcca tagaaagccg aggcatattt
     4321 cgccgcgtag gccatgatcg gcgtgtcggc aaatcccgca ccatcgagcg ccgcccgaat
     4381 cgcccgcacg cgcccgtcca tcatgtcgct gggagcgaca atatcggcgc cggcgtccgc
     4441 atggctcacc gccgcccgtg ccaaaagcgc cagggtcgga tcgttccgaa ttcggcctgc
     4501 atggcgcccg gccgtttcca ccaaaccgca atgcccatga ctcatgtact cgcacagaca
     4561 gacgtcagtg atcaccagga gcgacggcag ttcgcgtttg agaagacgca cggtcgactg
     4621 cacgatcccc tttgacgcat aggcctccga agcacgggcg tccttgcgcg ccgggatgcc
     4681 aaacagaagc accgccggca cgcccaactc gtgcgccgcc gccgcctcct tcaccaactc
     4741 gtcagccgaa agctggaaca ccccgggcat cgacccgatc ggatttcgca ccctccgacc
     4801 gggccgaacg aacagcggga gaaccagacg ctggacatcg agagtcgttt cctgcgccag
     4861 ccgcctcaag gcagcggact ggcgcagacg acgcgggcga tacaccggga attgcgcgag
     4921 cgaaggcaaa ttcatccggc aatgctgagc ccccgaaaac cggatgcaaa gcctttactt
     4981 gaccaaccgg aggaacatgg tcgccccgct ggtcggcagc gtcaccacgt tgcggtcacc
     5041 gctcacctcg accgtggcgt tcaccacggc ccaatcggga gccgccaggc tcgatgtgcc
     5101 ctgcacagcg aaccctgtcg ccgcggccgg ccaactaagc gtgacattgg tcccgacccg
     5161 ttcgatggcc agagggactt cgagcacagg cttgcaactt tcgagcaggt tgacccccgc
     5221 gttcgggccg caaggccgga ttacggcgta cttcaccgac gtgaagaaga ccttgtccga
     5281 gactgtaaac cgggcagccg cttgcgggcc ctggttcacc ccgttgaact tcatgtccgg
     5341 gatttcccaa tacgccgtcc gccaggtgtc cgtgccttcg agggtcacga gataactgtt
     5401 gtccgggaac gcaagcgtca gaacgccgtt gcgcacggtt tggtagacat ccggcttgaa
     5461 gttggcaccg acgagcgccg ggtcgtcgta gtaggtcacg cagatggcca gtcgggccgg
     5521 cggctgagaa ttgggcccga gggcttgatc gagtatggcg aagttgagat actggtgagt
     5581 gaagcccgga ctgccgtcct cgcgcgcggg ccgcacggcc atgcgaatgt cgcccgccgg
     5641 gccggcctcg gcaacaatca tctcctggtc gccgccgctg ttgccaacgt ccagtccgtt
     5701 cttgatccct tgtgccaggt ccaactcgac gaagctcccg tacttgtcgg tgcagatatt
     5761 cggatcctcg tagcaagtct cgagcgggtc ctgacccgcc agcggatggt cgcccacacg
     5821 caatatggcc aattcaaagc gtgaaacagc cacctgaccg ttctcggata tgaagcgcgg
     5881 gccggcggtg agcgcgcccg cattcacgcc cttgagattg acggccggca ccgtccagga
     5941 acgccgtatc caaacgcccg acccttccat cgtgtgacga ttggccgccg caacaaaacc
     6001 gatgcctccg gctgcgtcgg ttgcataggc ttccggaccg aataccaccc cgaggccggc
     6061 aaacgccggg tcgtcgtaga aatctacaca gaccttcacg gtgcgcgggt cgttgcacgg
     6121 caggcccagg tagttgtccg taatgccgaa attcaaatac aggccgtccg gcacaaccgc
     6181 tcgacgcttg tcgccggcag gccccacgtc ctcggcgtac gtcacggtct gatcgccgtc
     6241 gttgagcacc tgcatgtgat tgctggtcgc cgccgccacg tcgattccgg cgagattcgt
     6301 ttctggctca ggatcgcatg tctcggcagg caggaacttg ttgatgtctt ccggcgggcc
     6361 aaacgccccc tcctggccaa acgcgatgac tcgcacgatc agtccgggca ccgactccat
     6421 gcggatcgtc cctccgttca ccccgcccgc gcccgtgttc ccctgggccg cggccgaaag
     6481 cgaaccgcag aaacgctcgc cgtcggggcg aatgccattg ggaatccgga acagcaccca
     6541 gttccagcgc ttgttctttg catcgatcgg gatttgaccg ccaacgggag cgctcaaata
     6601 ggtgggcggc agggttccga tcagaaaatt gaagtcgcgg ggctggccct gagcgttaaa
     6661 cagagcggca tcaccgtaaa cctgcaccag gatgtcgatg aactcctcgt ccgcccactg
     6721 ccagtagtct gcgtcggcaa cgttgagaaa gctgccggtg accttcttgc ccgtgtggcc
     6781 gccaatcgag aagtcctcag tcacctggtc gcccccggtg agaatgttca gattctccac
     6841 ccaataaaca tctggcggct cgagaccgcc gtccgtgacc acgtaatgaa caaccttgct
     6901 ggaatccact gtcgcaggcc actgatccgg atcgaacgga ccggtctggg ccagacaaga
     6961 ggttgcgacg cctccgcaaa gcgccgccaa gacaaacacg caggaagttt tcataggctt
     7021 aatgtgacat tgtctgtgta cacctgaaac cctcggcttg tcaaagaaaa atacctctga
     7081 acaaagaagt agaagtaaac cggcgcgttc gcgggtcaat tcccgccctc gtttggcttg
     7141 agccacggag tgccgcccca gtaccacgtc tgagccttga ggctctcggc gtgggcgtcg
     7201 acgaaggcga cgttgctgcg tccgttgtgg cggaggtggg gtccgcccca attcgggtcg
     7261 ccgggcgaag tgacgcaacc ggtgcacggt tcatcggcct cagggtcgtg cacgatccag
     7321 gcgcccggct tctccttgga agccggagtg acacattgca gagggtctct ggtgcgcagc
     7381 ggttgggtgg cgctgtcggt gatttgcacc acgccgctgg ggcatttgac gtccgtatcc
     7441 cggattgcag gccactgggc cttgtcccag acgccaggcc aatcgcagcc gccgagacga
     7501 aagttggcgc cgtagttcga gacggcctgg tcggcgagat cggtgggata cacggccagg
     7561 aattccttga acttctgggt ccgggtcgga cagcggagga ggttcgtggt ctgctgatag
     7621 ggctggaggt agttgaacca ggccttgcga ttcaactggt tgccgatgag cgagaaggtc
     7681 cacgggaagc gcgactggtt gtcgtcggcg tagaccttgg atgccaccag catttggcgc
     7741 aggttgttga cgcaactgat cgagcggccc ttctccttgg cacgcgacag ggccggcaac
     7801 aacatgccgg ccagtatcgc gatgatggca atgaccacca gcaactcgat caacgtgaag
     7861 ccgccgccgg cgacgccatt ccgccgccgg ggccaatgaa ttatggtctt catccaatca
     7921 gtatctcctc cttcgcaacg tcgaatcgcg ccaccttctt ctcgaggaac gccagcatgg
     7981 ccatgttcaa cgcgacctgg acctggaaaa cgaactcgac gtcgcggacc ggtttctcgc
     8041 gcgttcggac gcagtggaga aagttcttcc aatgggcgcg ggttgcgtcg ctgcgttcgc
     8101 ctttgatctg ttggcgttcg ccattcttac gaatgatcgt gaggctgtca acgcccgaag
     8161 accagtctac agcacgatcg acctctcatc gattacttcc ggttttgtcg ggagcgccac
     8221 cgggccagat gcgaggcccg agggagggag tgtatcccct gag
//
LOCUS       DAOQRO010000131         8191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8301_length_8191_cov_4.609513, whole genome shotgun sequence.
ACCESSION   DAOQRO010000131 DAOQRO010000000
VERSION     DAOQRO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8301_length_8191_cov_4.609513"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1205)
                     /locus_tag="PKK20_06490"
     CDS             complement(297..1205)
                     /locus_tag="PKK20_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99568.1"
                     /translation="MQTPIQRNRPEHCGMSRPGRSVRLDPAAQRPAAFTLIELLVVIA
                     IIAILAGMLLPALGRAKSRALATACLGNLKQLQLCWQLYVDDNADRLPGNVALNPGDI
                     GSREGWTSDPTSWLQGNAWTDTTPIHLQRGVLYPYNHSLGIYRCPADKSTVRDQGRQP
                     RTRSYSMSMYMNFRPAPEDPEYVLCWHTLGQVQRPSPARAAVFIDENEKSIQQSAFGI
                     NARDYLTLFDSALWTWISFPATRHHNAGALSFADGHVELWRWLEPNTARIARLDDWTV
                     LKEAVPNADRDLDRFFGVVPEKVPIL"
     gene            complement(1213..1629)
                     /locus_tag="PKK20_06495"
     CDS             complement(1213..1629)
                     /locus_tag="PKK20_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99569.1"
                     /translation="MSDRRQQWKIALSLAALVTVSAGIGAVLGHRIARQQIDARNDPE
                     NWNDYVSREFERLIRPSPEQRPKVKSHLDNAVRQLQAIRIETVQRSTNVIWRLVADVE
                     RELTPEQRKAFEALKPQPSDLNLDVLNVTPADAPKP"
     gene            complement(1626..1916)
                     /locus_tag="PKK20_06500"
     CDS             complement(1626..1916)
                     /locus_tag="PKK20_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99570.1"
                     /translation="MKTFESRWQELTRHARAARVERPEAAAFGFARRVTVRAASANGT
                     TEDLWMRFCIRSLIGVSGVLVVLGAMEYSSARPASLMNPGVENTVAQLLGVL"
     gene            complement(1921..2505)
                     /locus_tag="PKK20_06505"
     CDS             complement(1921..2505)
                     /locus_tag="PKK20_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU99571.1"
                     /translation="MTDEAWTAEDYLRRLRAGDPDAAQGLVERLYPLVIRIVRGHLPT
                     RLSEEDVAQEVFLKLFARLDRYQARCGVPFEHWVARLTLRTCQDLLRAERRRPELRWS
                     DLSEDQRGWVEFLLSEKAQAPEAPDLAAAEVVERLLGQLTPADRYVIRLLDLEQKSVL
                     EASRLTGWSRTGVKVRAFRARRRLRKLAEALREP"
     gene            complement(2759..4135)
                     /gene="mnmE"
                     /locus_tag="PKK20_06510"
     CDS             complement(2759..4135)
                     /gene="mnmE"
                     /locus_tag="PKK20_06510"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNU99572.1"
                     /translation="MLNDTIAAISTPLGEGGLAVVRVSGPAALEVADHCFVPAGPSSP
                     KPSHAPSHSVHFGRILDQGRLADEVLLTVLRAPRTYTREETVEISCHGGALPARLVLA
                     AVLAAGARLAEPGEFTKRAFLNGRIDLAQAEAVADLIHARTELALSAAQEQLAGKLSQ
                     RIRRLRDDLLGILAHIEAQLDFADEDIAPDRRDQLLVRLDDGVASMDSLLRTADEGRI
                     LRRGVRAGIVGRPNVGKSSLLNQLLGHDRAIVSPIPGTTRDTIEETANIRGLPVVFTD
                     TAGLRHAKDAIEQEGIRRSRETAARAEVLLHVLDWSEPLEAEDIRFLDDCETRKRILV
                     LNKSDLPRRLDLPSAVASSTPTVAVSCLTGAGLESLKETLKKLIWTGDLRPEGLDVII
                     NARHQDALRRAREGAVRSRDALGRDLPLDVVALDLRIAVNAVGEIVGSTATDDLLDVV
                     FSQFCIGK"
     gene            complement(5011..5457)
                     /locus_tag="PKK20_06515"
     CDS             complement(5011..5457)
                     /locus_tag="PKK20_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99573.1"
                     /translation="MTTGFAVPFRLTGGEILVVSWFCCAVAAVVSYCLILHKLWTLIP
                     ANTARTTPVKAVGLLFIPLFNFYWNFVAFHGLAESLNALLKKSSIRDRVGEGLSLAYC
                     ILMCFSVVPIVGPLAALVGSVFCIVALRQMKDAGIALLQHGLVQSS"
     gene            complement(6028..6474)
                     /locus_tag="PKK20_06520"
     CDS             complement(6028..6474)
                     /locus_tag="PKK20_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99574.1"
                     /translation="MGTWLVDPSRCGSLGASELLARPVGDTNVPQMTLVISGTTILHR
                     YRKDRMTPFELVSPRADIKTSRDHSGVRYEAIEYSYYVVPNTSPHRIDLQFAGPLGQQ
                     SSYGIFRLQQGQLWFCFPAVTGDSRRPRAWDTDAGATRVIRATRVD"
BASE COUNT         1595 a         2537 c         2630 g         1429 t
ORIGIN      
        1 gcctgccggc aggcaggcgt cgccgctccg caccggttca tggtcatcgt gtacaccgtg
       61 gaggggggcg cggccgctgc agcccgcgca cagaaccgcc agtccagcgg ccaagaccat
      121 ggagactgtc gagatgcacc cgcgtccgat ggggcgcccc cttagggtct gtgcaaaaat
      181 caatttcact gagcggttca aaaagcaacg ggtcgcttcc cacgtgccag gagcgtgccc
      241 aaccgccggg cggagttcaa ggcgcaaccg ggaaaaagga tctcgagatc tcgaagtcag
      301 aggatcggga ctttctccgg aacaacaccg aagaaccggt cgaggtctcg gtcggcgttc
      361 ggaacggcct ccttgagcac ggtccagtcg tcgagccggg cgatgcgggc ggtgttgggc
      421 tcgagccaac gccagagttc gacgtggcca tcggcgaagc tcaatgcgcc ggcgttgtga
      481 tgccgggtgg cgggaaaact gatccaggtc cagagcgcgg aatcgaagag cgtgaggtaa
      541 tcgcgggcgt tgatgccaaa agcgctctgc tgaatgctct tctcgttttc gtcgatgaac
      601 accgcggcgc gggccgggct gggcctctgg acctgcccca gcgtgtgcca gcaaagcaca
      661 tactccgggt cttccggggc ggggcgaaag ttcatgtaca tgctcatcga gtagctgcgc
      721 gtgcgcggct gcctgccctg gtcgcgcacg gtggacttgt cggccgggca cctgtagatg
      781 ccgagcgagt ggttgtaggg gtacaagacc ccgcgctgga gatggatggg ggtggtgtcg
      841 gtccaggcat tgccttgcag ccaggacgtc ggatcggacg tccagccctc acggctgccg
      901 atatcgcccg ggttgagcgc cacattgcca gggagcctgt cggcgttgtc gtccacgtac
      961 aactgccagc agagctggag ctgcttgagg ttgcccagac aggcggtcgc gagcgctctc
     1021 gatttcgccc gacccaaagc gggcaagagc atgccggcga ggatcgcaat gatggcaatc
     1081 accaccaaaa gctcgatcag ggtgaatgcc gccggccttt gtgccgccgg gtccagtcgc
     1141 acgcttcggc cgggtcggga catgccgcaa tgctcaggtc ggtttcgttg aatcggtgtc
     1201 tgcatgctgg gcctatggtt taggggcgtc cgcgggagtc acgttgagga cgtccaggtt
     1261 caagtcggac ggctgcggtt tgagggcttc gaatgccttg cgttgctccg gggtcaactc
     1321 gcgctcgaca tcggccacga ggcgccagat cacgttggtg ctccgttgga cggtctcgat
     1381 ccggatcgcc tgcagctggc ggaccgcgtt gtccaggtgg ctcttgacct tcgggcgctg
     1441 ttccgggctg ggccgaatca ggcgctcgaa ttcgcggctg acatagtcgt tccagttctc
     1501 cgggtcgttc cgcgcatcga tttgctgtcg tgcgatgcga tgaccgagca cggccccgat
     1561 gccggcgctg acggtcacga gggccgccag gctgagggca atcttccact gttgccgccg
     1621 atcgctcata agacccccaa aagctgcgcc acggtgtttt ccacgcccgg attcatgagg
     1681 gaagccggcc gcgcgctgct gtattccatc gccccaagca ccaccaaaac gccgcttacg
     1741 ccaatcagtg accgaatgca aaaccgcatc cagagatcct ccgtcgtgcc gttggcggac
     1801 gccgcccgca cggtgacgcg acgcgcaaaa ccgaaagcgg cggcctcggg ccgctcgacc
     1861 cgagcggccc gcgcatgccg ggtcagttct tgccaacgag attcgaacgt cttcatggat
     1921 tcacggttcc cgcagagcct ccgccagctt gcgcaggcgt cggcgggccc gaaaggcccg
     1981 caccttcacc ccggtccggc tccatccggt cagccggctc gcttcgagca cgctcttttg
     2041 ttccaaatcc aggagccgga tgacataccg atcggctggc gtcaactgcc cgagcaaccg
     2101 ctcgacgacc tcggcagcgg cgaggtcggg cgcctcgggc gcctgcgcct tctccgagag
     2161 gaggaactcc acccagcccc gctggtcttc gctcaaatcg ctccagcgca attcgggccg
     2221 acggcgctcg gcccgcagga ggtcctggca ggtgcggagg gtcagacggg ctacccagtg
     2281 ctcgaacggc acgccgcacc gcgcctggta acggtccagc cgcgcgaaca gcttcaggaa
     2341 cacctcctga gccacgtcct cctcgctcag gcgtgtcggc aaatgacccc gcacaatgcg
     2401 gatgaccagc ggatagagcc gctcgaccag cccctgcgcc gcgtccgggt cccccgcccg
     2461 gagccgccgg agatagtctt ccgctgtcca tgcctcgtcg gtcatgggtt gccattcaca
     2521 ctaggataac gccgccgcgt gcgcgccggt tacatgtcgg gtgcttccct ctcgcagccc
     2581 actggagaac tgacccccga acaacttgta cccagtcgta ttcgtaatcg taatcgtaat
     2641 cctcggtcgc cgattgggat tacgatgagg agaatccgcc acatagtcct cgtgcgcatt
     2701 cacccgctga gtgtcggcgc tttacggaat ccaccccgcc cggcgcaccg tcgcccttct
     2761 atttgccgat gcaaaactgg ctgaagacga catcgaggag atcgtcggtg gcggtggagc
     2821 cgacgatctc gccgaccgca ttgacggcaa tccgcaggtc cagggccacg acatcgaggg
     2881 gcaaatcccg gccgagtgcg tccctcgacc gcacagcgcc ctcgcgcgcg cgcctcaacg
     2941 cgtcctggtg ccgggcattg atgatcacat cgagcccttc gggccgaaga tctcccgtcc
     3001 agatcagttt cttaagcgtt tccttcaggc tctcgagccc cgcaccggtc aggcacgaca
     3061 cggcgacagt tggagtcgag gacgccacgg ccgacggcag gtccagacgc cgtggcagat
     3121 ccgacttgtt gagaaccaga atgcgtttcc gggtttcgca atcgtcgagg aaccggatgt
     3181 cctcggcctc aagcggctcg gaccagtcca ggacgtgcag caacacctcg gctcgggccg
     3241 cggtttcgcg gctgcgccgg atgccttcct gctcgatggc atccttcgcg tggcgcagcc
     3301 ccgccgtgtc ggtgaaaacc accggcaatc cgcggatgtt ggcggtttcc tcgatcgtgt
     3361 cgcgggtggt tccggggatg ggcgagacga tagcgcgatc gtgaccaagg agttgattga
     3421 ggaggctgga cttgccgaca ttcgggcggc cgacgatccc ggcacgcacg ccgcgccgca
     3481 gaatcctgcc ctcgtcagcc gtccgaagga gggaatccat ggaagccaca ccgtcgtcca
     3541 accgcacgag caattgatcg cgccgatccg gggcgatgtc ttcgtctgcg aaatcgagtt
     3601 gggcctcgat atgcgcgagg atgccgagca gatcgtcgcg gaggcggcgg atgcgctgcg
     3661 agagctttcc ggcgagctgt tcctgggcgg cgctcagagc cagttcggtc cgcgcatgga
     3721 tgagatcggc caccgcctcg gcctgggcaa ggtcgatgcg gccattgagg aaggcgcgct
     3781 tggtgaactc gcccggttcc gccagacgcg cgccagcggc cagaaccgcc gctaaaacca
     3841 gccgcgccgg cagggccccg ccatgacaag agatttcaac agtctcctcg cgcgtatagg
     3901 tccgcggcgc ccgcagcacg gtgagaagga cttcgtcagc cagccgcccc tggtcgagga
     3961 ttcgtccgaa gtggacgctg tgacttgggg catggctcgg tttgggcgag gaggggcctg
     4021 cggggacaaa gcagtggtcg gccacttcga gggccgccgg acccgagacc cgaacgacgg
     4081 caaggccgcc ttccccgagg ggtgtcgaga tggcggcgat cgtatcgtta agcatgggcg
     4141 gacgcgaatt cacggggcgg aagaaacaac gcggtcacaa cgcccgccgg cgcagggcgg
     4201 agtgcgcgtc cgccccataa ccgcggcgag accgggtatc gtgttcggca gagccgttca
     4261 ttcgggcgaa gaatagcacg cctcgatgcg ggcacgagcg ggaacttcgc gactattcgg
     4321 agggcgccaa ggtccaggag ccgctcatcg ccttgctgag ggaccgctaa ccgccgggct
     4381 tccgctgcca gccgaccgag acgagggtct cgagcggtgg aggtggtccc ccgcgcgctg
     4441 gagaccgaag aggaccagtt ccttcaggct gcggctcggg ccgtccggcc tcggccgcac
     4501 tggcttaagg gcctgtgcaa aaatcaatcg gaagtaattc atgcactgac gccaaggact
     4561 acgccctgaa ccacggtcac tacatcagca gcgaaccgaa gaaggcatgc aggctgtctt
     4621 cgccgagacg ttcttctacc tgaccctgct gaacgagcgg gagactcctc tcccctccgg
     4681 gccacccccc ggtaacggac aactctaaca cgtgggctgt cgcaaagaac aggagcgtcg
     4741 cgacaaatcc gaccgccagg aggatcatca ctccccgaaa tgccgaaatc ctttgcccaa
     4801 cccgccaaag cacgtaagct gagataagaa aatctgatca agcagctccc gccagtcaac
     4861 gaaaaacaga ccctgagatc tccgacgctc cgactacccc agcccatccc cgagggcact
     4921 gcaaggcgca cagcagctct cgcaacctcg agccagattg attgacgacc cctttccgag
     4981 gcatgcgagc tgttcgatgg cttcctttct tcatgacgat tgcacaagcc cgtgctgcaa
     5041 aagcgcgatc cccgcatctt tcatctgcct gagggctacg atgcagaaaa ccgatccaac
     5101 caacgcagcg agagggccga ctatgggcac cacggagaaa cacatcagga tgcagtacgc
     5161 caggctcagg ccctcaccga ctcggtctcg gatcgaactc ttcttcaaaa gggcattaag
     5221 cgattcggcg agcccgtgga atgcgacaaa gttccagtag aagttgaaga gtgggatgaa
     5281 gaggagtccg acggccttga ccggagtcgt gcgagctgtg tttgcaggta tcagcgtcca
     5341 cagtttatgc aggatgagac aataggaaac gactgctgcc accgcgcaac agaaccagga
     5401 caccacgaga atctcgccgc cagtcaagcg aaagggaact gcgaaaccag tcgtcatcaa
     5461 caggggtaat aacagaactg ccccggcaag catacacacc caaaacaggg tgaaccaact
     5521 gctgagacgc ttaacatcgg taaccgccag gaagcgacca ggagaggatg attgaccaag
     5581 agacagaggt gcagcggggg ggcttagtgc agcaggcaca gacactgcca ccgggccctg
     5641 tgccactgga cgaggtggcc gctcaggcac cttgatcttg acgccgcagg cagggcagtc
     5701 gatcggaagc ccgcagcaac gctgctcaca agcgatgtgc tgaccgcatc gtggacaggc
     5761 gaacttgaaa tcgctggcgg aagctggacc tctgcttctc gacgaggagt ccgccggggc
     5821 atgcggcacc ctgatcctgg cgtggcacgc tggacagtcg atttggagtc cgctgcaaac
     5881 ccgctcgcag gcgatgtgct ggccgcagct tggacaagcg aacttgaaat cggtggtgct
     5941 caatgattcg gtgtcggcgt tattcataat ctgccgcccg gacttcagga ggacgtcgaa
     6001 ttcagtcgct gcgggttcat gtgtgcatca atcaactcga gtggcgcgaa tgacacgtgt
     6061 cgcgccagca tcagtgtccc aggctcgtgg gcgccggctg tcgcccgtca ccgcgggaaa
     6121 gcaaaaccag agttgtcctt gttgaagccg gaagattccg tacgacgact gttggcccaa
     6181 cgggccggca aactgcaaat cgatgcggtg gggcgacgta ttgggcacca cgtaatacga
     6241 atattcgata gcctcgtacc ggacaccgct gtggtcgcgg cttgtcttga tgtcggcgcg
     6301 tgggctgacg agctcgaagg gggtcatccg gtcttttctg tagcgatgca ggatggttgt
     6361 gcctgagatc accagggtca tctgcggcac attcgtgtca cccacgggcc gggcgagcag
     6421 ttcggaggcg cccagcgaac cacagcgcga aggatcgacc agccatgttc ccatgatcga
     6481 gccgacaact cggctttccg agccaccgtg cgaaggagac tgccaaaagg gagaggcact
     6541 gattgcgccg ggagaggtgc gcgtccgccc tcgcgaggct ccgaaaagcg gttgattcgc
     6601 gccaattcgg cgttcggcgg cctgctgtaa accacgcgat gcctctcccg agggaatccg
     6661 ccgggcaagc caaagagcta cggtgattgc gccaagcgcc gccaagacga cgggaacacg
     6721 cgagcgtccc aagctgagga ggcgctcaag cgcagagggt cgccgcttcg gtgcaagaaa
     6781 gaccagagaa cagaccgggc actggacgct ggcaccgagc ttggcgtggt ggacttggat
     6841 gggtgaatga catttggggc actcgaagcg atcggcgcgc atccgatcag tgccggccgc
     6901 ggagccgtgg ggtgggctcg tgacaggatc gggcatgtcg ccaccggcag gaatggaagc
     6961 ttcttgccca caggccggac aggtggtttg catgccggcc atatccggat cagcaccgat
     7021 ctgccgaccg cattgtgggc agggaaaggt catgcgatgt gagacaacgt cctctgaggt
     7081 gcttcttgcg cccacagccc tgcactcacc tgtgaccgcc tcgtggacgt tgcgtctttc
     7141 ctgagaagca ccagcatggc tgagtctgat accacacgat ggttgttgcc tgcaattctg
     7201 gttttccagt cgatcagaac caagtcggca atcaggcaat caggcaatca gtcctttgtg
     7261 attttatctt tgacacttgc accaattggc caagaattca agccttattg gccgacgctt
     7321 cagatgcggt ttggtcgatg cccggtgccg gcctgagagc gacggcgatg ccaaggcatg
     7381 aggatgttct gccaccactg cgggcaggtc gttgacgctc ccgatattcg ggccgacgct
     7441 gcggtccgct gtccccggtg tctggtgcta acccacgtcc ctgggagtgc gacacggtcc
     7501 acgatggagc tttctggggc ttacccgagg gttggcctgg gctggccggg atacgtggtt
     7561 atagcgctga ttgtcgcggc aacggcggtc ctcttcgttt ttttccgggg gcatgtccag
     7621 cagcaggtgt tgagtcgagt cctcccgagg gccaggccgg gggcaatccg ggtgctccac
     7681 ttttattgta cgaacgcact gccggccgga acgaacagtt cgacgctgac tctgggcacg
     7741 cccaaggaga aggcgggccg ggcctacttg gtcgtccaag tttccgtgga ttcagccgcg
     7801 gctgaggtcg aacgcctcac tgaacgcgag ctcgaggaag tcagacaggc aaatccgaac
     7861 gccagaatga caaacgtttg ggtaagttgg ccgagcgcca gatggttcag gatcgtcggt
     7921 ccgcggcacg ctctgtccac agcttgcgcc caccaggctg gcccggggca gttctccgag
     7981 gcgccggcat ggaactcacc accatccccg gacaggccgg agcgcgttga gtacacggtt
     8041 tgttttgagg tcgagcggag gctggccgag gcaggacggc tgtcctttca atacaggtgg
     8101 aggcgccccg tgccactcaa gcggatcgag ggggaagctg ggacgtgaat cccatatgtc
     8161 catactgccg caggccaatc gaggggaacg a
//
LOCUS       DAOQRO010000132         8172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8331_length_8172_cov_5.151780, whole genome shotgun sequence.
ACCESSION   DAOQRO010000132 DAOQRO010000000
VERSION     DAOQRO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_8172_cov_5.151780"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3397)
                     /locus_tag="PKK20_06525"
     CDS             complement(<1..3397)
                     /locus_tag="PKK20_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99575.1"
                     /translation="MDLRRASPGLALALCLATMAGEHRGAIAIYRDSIPAPAASASPE
                     HLAGWLEGADFETAFLTSEDLADTARLDRARFDVVVLPYGPVFPVSAADTFRRFLRAG
                     GKLFTTGGYAFDHLIEREPDGWRAYQPPPPPRQQGVAWFCDLPAAGLRGRGALTFRGL
                     LKSANVTGPGFAHFSVYQFAADGSLLAWRDLCQVRGTRDWEEHVYTFDLHPQASTVSL
                     RAGLYQCRGAAWFDAVQLDDAAGNPLLEAQFEEPPHPDRRDPSNWWRSDAERCAWRAD
                     QGRDGSGALEATLGFELPRIERLNTRHGHPADGLEVEPTQLGLFQADYPLVRAVSVRA
                     ARGQSLLPPDLAVSAPVEGWAACGVVGWNTARWIPLLNAHDSYGRLRGAAGALLRHYG
                     GQWRGSSWAFFGVTNLNLFAPAQPGMSNAFLGIVRSLVDDAYIASLTTDHDCYRQGEE
                     VGFNALVFNGGRAARTLRVAVAIHAGEPADPAVYGENLLVETPDRPRAAQEPVATLRF
                     DVAVGPGQTNLVSGRWKPARFESDFYHLVAHLADGTNALDQIAGGFVVRDERTIASGP
                     RLTYRDNYLRFGDRPLFQFGTDDWSYVFTTARETPLQWLEDMRLRRDLGVTLYENLQF
                     GLPRSAAHEEQLLRKVDGLVQLAQKYGQVYFAGLLVGYNAAASDTDLERQRDYCARFA
                     RRYSSVPGLIYYLNGDYRCEPGDAVTPQWNDFLRDRYGGNDALRQAWGTRAPAQPLGA
                     IPSVNSWDWDRTWDDVSAYDENCFRAWLIRRWNREMIAGIRQHDTNHPTSGEFYQLPH
                     GGVDLPAGIDGLDLANFGFFDKPGFDLARFPALCKLNDQRARDKSGGPGEYGVKTHPA
                     WGDGRDYGYHTARTPEQAIDLFLAIPHAALGLGASRIHNWCWKDDAHRVFPWGMVYPC
                     DGVPKDTACVHRNLSLLFRHFAPVYREPQVYVLTPDCHRMGGGKWRVTDGILASMDIA
                     LEAHVHNLGTLNEQDGRIPASARVLFYPIPYCLSDATYGTLLRWVDQGGVLYLSGDFC
                     YDARRQRSRPNRLTELAGVRRLSANPSPLAVDPGKAVDCPELEVEPVTATVLRRAPGG
                     AVLLVENRVGRGRVFYTPDPIELHSTPDRRPTDVALYRRVLEAG"
     gene            complement(3549..4352)
                     /locus_tag="PKK20_06530"
     CDS             complement(3549..4352)
                     /locus_tag="PKK20_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNU99576.1"
                     /translation="MKPAAQPLTPRRVNVLGVGVSVLNLRTAVETLTEAVGQRRKGYV
                     CVSNVHVLVEAQYNPAYKRVLNRAFLVTPDGMPLVWAGRLAGYREMGRVYGPELMLAV
                     FEATRTTGQTHFLYGGMPGVAEDLKNRLEARFPGVRIVGTGCPPFRPLSAKEETELEA
                     QVRAARPDFFWTSISTPKQDLFNAALLPRLDTALMIGVGAAFDFHTGRVAQAPRWIQR
                     SGFEWLYRLCREPRRLWKRYLTVNPVFLALVLCQITGLKKFTLDDNPHV"
     gene            complement(4349..4852)
                     /locus_tag="PKK20_06535"
     CDS             complement(4349..4852)
                     /locus_tag="PKK20_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99577.1"
                     /translation="MKPDTKPQLAPPAGNSEPGRTPRNRRQFIQDTAPLVGAAALSAS
                     AVGTAAADARIVLGLETHPNAMTALGGKFFFDDDQEFPYTQCVVFEYDTGGKRRQLIC
                     EHRTWSPYVQEGHENGNVFYGTRGTPILGKQAGWKLFGSRNKLMNRLLGRAYREGHWA
                     APKEITA"
     gene            complement(4929..8072)
                     /locus_tag="PKK20_06540"
     CDS             complement(4929..8072)
                     /locus_tag="PKK20_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNU99578.1"
                     /translation="MLHRLIDASLRQRAFLLIVAAVSIFIGLWSANNLPIDAVPDITS
                     PQVQVNTEVITLAPEESEQAVTIPIEMELAGIQGVQEMRSLTKFGLSQVTLIFADRTD
                     VYRARQLVAERLQAVVDRLPPGLTPKLAPISTGLGEIYYYTLAFRTDATNRPADRIEE
                     LRELREVHEFVVKPMLRGTPGIAEINANGGYEKQIVVQPRPNDLERAGLTFADLAALV
                     RENVENAGGGVIHNGPDQLTVRTLARVQSAQDIADLPVKFAAAVKPLLVRDVADVDVG
                     SSFRTGAATENGEEAVLGTAMMLAGENSRIVAQRFGARLREIQQHLPPGLDLRTQYDR
                     ADLVDATVATVRKNLFEGAILVVAVLLAMLGNWRAALIVSAAIPLSFLWALTGMVRFG
                     ISGNLMSLGAIDFGLIIDGAVVMVENIVRQLAGKQKHLGRVLTPVERLHTVGIASKQV
                     AHPMFFGVLIITIVYVPILALTGIEGKMFHPMAITVMLALGGALVLALTLMPVLCSCL
                     LGGHIREGDNILVRWIKRAFEPSLRLALAKPWLVGISTVLWLALSTVAFLRLGAVFVP
                     QLDEGSHTVMVYRTNSMNLDASLAMEMATEKLLLTIPEVDRVFCRLGTSEVATDPMPP
                     SQNDLYIFYKPRSTWRRINGRPMTKSDLAALIEETVAKELPDQSFLFAQPIEMRFNEL
                     IEGVRSDLSVKIIGTDFDVMEGLAAQVKQLLEHVPGAGDIEFETQGRAPVLEIVVDRD
                     ALRRYNLHAANVNATVSTALAGETVGMIYEGNRRFEIVVRLEGDLRDRLDTLRSLPVR
                     VGEHGLVPLEKVAAFRTVASVDPIVRENGARRVALMVNLRGRDVESFVRDAEARIREQ
                     VTFPDGYVYEFGGEFKNLQQARTRLLIVVPAALVLIFLLIFMAFGSLRQAIVIYTGIP
                     LAVTGGVIALWLRGMPFSITAAVGFIALSGVAVLNGVVLISYFNELREQGRTVAQAVL
                     EGALIRLRPVLMTAFVASLGFVPMALATGPGAEVQRPLATVVIGGILSSTFLTLILLP
                     LLYRFAENIGRAHAAPD"
BASE COUNT         1430 a         2526 c         2820 g         1396 t
ORIGIN      
        1 ctcccgcctc gagaacccgc cggtagagcg cgacatcggt cggacgccga tccggcgtac
       61 tgtgcagttc gatcggatcg ggcgtgtaga acacccgtcc ccgcccgaca cgattctcga
      121 cgagcagcac ggcgccgccg ggcgcgcgcc tcagcactgt ggccgtgacg ggttcaactt
      181 cgagttcggg acagtcgacg gctttgccgg gatcgaccgc cagtggagag ggattggccg
      241 agagccgtcg caccccggcc aactcggtca gccggttcgg acgcgagcgc tgcctccggg
      301 catcgtagca gaagtcgccg gacaggtaga gcacgccgcc ttgatcgacc cagcggagca
      361 gggtcccgta ggtggcatcg gagagacaat acgggatggg gtagaagagc acgcgggccg
      421 aggccgggat tctgccgtcc tgctcgttaa gcgtgccgag gttgtggacg tgcgcctcga
      481 gcgcaatgtc catgctggcc aggatgccgt cggtgactcg ccactttcca ccacccatgc
      541 ggtggcaatc gggagtcagg acgtagacct gcggctcgcg atatacgggg gcgaagtggc
      601 gaaagaggag gctgagattg cggtggacgc aggcggtgtc tttgggcacg ccgtcgcacg
      661 gatagaccat gccccacggg aacacgcgat gggcatcgtc cttccagcac caattgtgga
      721 tgcgggacgc gcccaggccg agcgcggcgt gcgggatggc gaggaacagg tcgatcgcct
      781 gctccggggt ccgcgcggtg tggtagccgt agtcgcgccc gtcgccccag gccgggtgcg
      841 tcttgacgcc gtattcgccc gggccgcccg atttgtcgcg ggcgcgctgg tcgttgagct
      901 tgcagagggc cgggaaccgg gccagatcga atcccggttt gtcgaagaaa ccgaagttgg
      961 cgagatcgag gccgtcgatg cccgccggaa ggtcgacgcc gccgtggggc aactggtaga
     1021 actcgccgga tgtgggatgg ttcgtgtcgt gctggcggat gcccgcgatc atttcgcggt
     1081 tccagcggcg gatcagccag gcccgaaaac agttctcgtc gtacgccgag acgtcgtccc
     1141 aggtccgatc ccaatcccag gagttcacgg atgggatggc gccgagcggt tgggcggggg
     1201 cgcgcgttcc ccaggcctgg cggagggcat cgttgccgcc gtagcgatct cgcagaaagt
     1261 cgttccattg cggggtaaca gcatcgcccg gctcgcacct gtaatcgccg ttgagatagt
     1321 aaatgagtcc cgggacgctc gaataacggc gggcgaagcg ggcgcagtag tcgcgctggc
     1381 gctcgaggtc ggtgtcgctt gccgccgcat tgtagccgac gagcaatccg gcgaagtaga
     1441 cctgcccgta tttctgggcc aattgcacca aaccatccac cttgcggagt aactgctcct
     1501 cgtgggccgc ggaacgcggc aggccgaact ggaggttttc gtagagggtg acgcccaggt
     1561 cacggcgcag gcgcatgtcc tcgagccatt gcagcggggt ctcgcgcgct gtggtgaaga
     1621 cgtaactcca gtcgtcggtg ccgaactgaa acagcgggcg atctccgaag cgcaggtagt
     1681 tgtcgcggta ggtcaggcgt ggaccggacg cgatggtcct ttcgtcgcga acgacaaacc
     1741 cgccggcgat ctggtcgagg gcgtttgtgc catcagccag gtgggcgacg agatggtaga
     1801 agtcagattc aaaccgggcg ggcttccagc gcccggagac gaggttggtc tggccggggc
     1861 cgacggccac gtcgaaccga agagtcgcca cgggctcctg tgcggcccga ggccgatcgg
     1921 gcgtttccac gagtaggttt tcgccatata cggccgggtc ggctggctcg ccagcatgga
     1981 ttgcgacggc gacgcgcagg gtgcgcgccg cgcgtccgcc attgaatacc agggcgttga
     2041 aaccgacctc ttctccctgg cgatagcagt cgtggtcggt ggtcagcgag gcgatgtagg
     2101 cgtcgtcgac aagggatctg acgatgccga ggaatgcgtt tgacatgccc ggctgggccg
     2161 gggcgaagag gttgagattc gtgacgccga agaacgccca actcgaaccg cgccactggc
     2221 cgccgtagtg ccgcaacagc gcgccggcgg ctccccgcaa tcgtccgtat gaatcatgcg
     2281 cattgaggag tggaatccag cgcgccgtgt tccaacccac gactccgcag gctgcccacc
     2341 cttcaaccgg ggccgaaacg gcgaggtccg ggggtaggag ggactggcct cgagcggctc
     2401 tcacggacac ggcgcgcacc agcgggtagt cggcctggaa gaggcccagt tgcgtcggtt
     2461 cgacttcgag tccgtctgcc ggatgtccgt gccgcgtgtt gagtctctcg attcggggaa
     2521 gctcgaaccc gagggtggct tcgagggcgc cagagccgtc ccgcccctgg tcggcgcgcc
     2581 aggcgcaacg ctccgcgtcc gaacgccacc agttggacgg gtcgcgccgg tccgggtgcg
     2641 gcggctcctc gaactgcgct tccagaaggg ggtttcccgc ggcgtcatcg agttgcacgg
     2701 catcgaacca agccgcgcca cggcattggt agaggcctgc gcgaaggctc accgtcgagg
     2761 cttgcgggtg caaatcgaac gtgtagacgt gctcctccca gtcacgcgtg ccgcgcacct
     2821 ggcagaggtc ccgccaggcc agcagcgatc catccgcggc gaattgatag actgagaaat
     2881 gggcgaagcc gggtccggtt acgtttgcgc tcttgagcag gccgcgaaac gtcagggcac
     2941 ctcgcccgcg caggccggct gcaggcagat cgcaaaacca ggccacgcct tgctgccgcg
     3001 gcggcggcgg aggttgatag gcgcgccatc catcgggctc ccgttcgatc agatgatcga
     3061 acgcgtagcc gccggtcgtg aacagtttgc cgccggcccg gaggaatctg cggaaggtgt
     3121 cggctgccga tactgggaac accgggccgt aaggcagcac gacgacatca aagcgagcgc
     3181 ggtcgagtcg ggcggtgtca gccaggtctt cgcttgtcag aaacgccgtt tcgaaatcag
     3241 cgccctcgag ccatcccgcc aggtgctcgg gcgaggccga ggccgcgggt gcggggatgc
     3301 tgtcgcggta gattgcgatg gccccccggt gctcacccgc catggtggcc aggcagaggg
     3361 ccaaggcaag gccaggcgag gcgcgcctca ggtccatccc aatcctcagc ctcaaggcct
     3421 gtcccaacgc cttccagcct ctctcctccc gcgcctcgct ccttggcccg ggtttctcac
     3481 catcgggaag atgacaaaga aagccgggct gtgtgcgatc tgatgttcgg gttgtggcgg
     3541 cagggtgact atacgtgcgg gttgtcatcg agtgtgaact tcttcagacc cgtgatctgg
     3601 cacaagacca gcgccaggaa gacgggattg actgtcaggt agcgtttcca gaggcggcgc
     3661 ggctcgcggc agagccgata gagccactcg aagccgctcc gctgaatcca gcgcggcgcc
     3721 tgggccacgc gtcccgtgtg aaaatcgaat gccgccccga cgccgatcat gagggccgtg
     3781 tcgagacgcg gcagcaacgc ggcgttgaac aggtcctgtt tgggcgtgct gatgctggtc
     3841 cagaagaaat cggggcgcgc ggcgcgaacc tgcgcttcga gttccgtttc ctccttggcg
     3901 ctgagcgggc ggaagggggg acaaccggtc ccgacgatcc gcacgccggg gaatcgggct
     3961 tcgaggcggt tcttgaggtc ctccgccacg ccgggcatgc cgccgtagag aaaatgagtc
     4021 tggccggttg tgcgggtcgc ctcgaagacg gccagcatca gttccggtcc gtacactcgc
     4081 cccatctcac ggtatccggc gagccggcct gcccagacca gcggcatgcc gtcgggcgtg
     4141 accaggaacg cccggttgag cacccgtttg taggccgggt tgtactgtgc ctcgacgagc
     4201 acatggacat tcgagacgca gacataacct ttgcgccttt ggccaacggc ttccgtgagg
     4261 gtttcgaccg cggtccgcag gttgagcacc gaaacgccga caccgagcac attgacgcgc
     4321 cgaggtgtca gaggctgcgc cgcgggtttc atgccgtgat ctcttttggc gctgcccaat
     4381 gcccctcgcg atacgcgcgt ccgagaaggc ggttcataag cttgttgcgc gagccgaaga
     4441 gcttccagcc ggcctgtttg ccgaggatcg gcgtgccacg ggtgccatag aagacgttgc
     4501 cgttctcgtg tccttcctgg acgtaaggcg accaggtccg gtgctcgcag atgagctggc
     4561 gccgcttgcc gccggtgtcg tattcgaaca cgacgcactg ggtgtagggg aactcctgat
     4621 cgtcatcgaa gaagaactta ccgcccagcg cggtcatcgc gttgggatgc gtctcgaggc
     4681 cgagcacgat tctggcatcg gccgcggctg tgccgaccgc ggaggccgac agcgcagccg
     4741 cgccgacgag cggggctgtg tcctgaatga actggcggcg attccttggt gtgcggcccg
     4801 gctcggaatt gccggcggga ggggcgagtt gcggtttggt gtccggtttc ataggctcga
     4861 tcgggggatg atgcgaggga cgtttcgatt gtgtggtgtg tcgtgatcct gtgctgcggt
     4921 gtccagggtc agtcgggggc ggcgtgggct cgcccgatgt tttccgcgaa tcggtaaagc
     4981 aaaggcaaca ggatgagggt gagaaacgtc gagctgagga ttccgccgat gaccacggtt
     5041 gccagcgggc gctggacctc ggcgccgggg ccggtggcca gggccatggg cacgaagccg
     5101 agactggcca cgaaggcggt catgagcacg ggccgcaagc ggatcagcgc cccttcgaga
     5161 acggcttggg cgacggtgcg accctgttcg cgcagttcgt tgaaatagct gatcaggacc
     5221 acgccgttga gaacggccac accgctcaag gcgatgaagc cgaccgcggc ggtgatgctg
     5281 aagggcatgc cgcgcagcca cagggcgatc acgccgcccg tgactgccag aggaatgccg
     5341 gtgtagatga cgatcgcctg gcgcaggctg ccgaaggcca tgaagatcag caggaatatc
     5401 agcaccagcg ccgccgggac gacaatcagc agccgggtgc gcgcctgttg cagattcttg
     5461 aactcgccgc cgaactcgta cacgtacccg tccgggaaag tgacctgctc gcgaatgcgc
     5521 gcctcggcat cgcggacgaa gctctcgaca tcgcgtccgc gcaggttgac catcagggcc
     5581 acacggcgag ccccgttctc ccggacgatg ggatcgacgc tggccacggt gcggaaggcg
     5641 gccaccttct cgagcggaac cagtccgtgc tctcccaccc gcaccggcag cgatcggaga
     5701 gtgtcgaggc ggtcgcggag gtcgccttcg agacggacga caatttcgaa ccgacgattg
     5761 ccctcgtaga tcatgccgac ggtttcgccg gctagagcgg tcgagacggt ggcgttgacg
     5821 tttgcggcgt gcaggttgta gcggcgaagc gcatcccgat cgaccacgat ctcgaggacg
     5881 ggcgcgcgtc cttgagtttc gaactcgatg tcgcccgcgc ccggcacgtg ttcgaggagc
     5941 tgcttcacct gggctgccaa cccctccatc acatcgaaat cggtgccgat gatcttgacg
     6001 gacaggtcgg accgaacgcc ttcgatcagt tcgttgaacc gcatctcgat cggctgggca
     6061 aagaggaaac tctggtcggg aagctctttg gcgaccgttt cctcgatcag cgcagccagg
     6121 tcggacttgg tcatcgggcg cccgttgatg cggcgccagg tcgagcgcgg tttgtagaag
     6181 atgtagagat cgttctggct tggcggcatc gggtcggtgg ccacttcgct cgtccccagg
     6241 cggcagaaga cacggtccac ctcgggaatg gtgagcagca gtttctccgt ggccatttcc
     6301 atcgccaggg aggcgtcgag attcatgctg ttggtccggt agaccatcac cgtgtgcgag
     6361 ccttcatcga gctgcggcac gaacacggcg ccgagccgca ggaacgccac cgtcgagagc
     6421 gcgagccaga gcacggtgct gatcccgaca agccagggtt tggcgagcgc caggcgcagg
     6481 gacggctcga acgcgcgttt gatccatcgc acgaggatgt tatccccctc gcggatgtgg
     6541 ccgccgagca ggcaggagca gagcacgggc atcagggtca gggcgaggac cagcgcgcct
     6601 cccagcgcca gcatcacggt gatcgccatc ggatggaaca tcttcccctc gatgcccgtg
     6661 agcgccagga tgggcacgta gacgattgtg ataatcagca ccccgaagaa catcgggtgc
     6721 gcgacctgct tgctggcgat cccgacggtg tgcaatcgct cgaccggcgt cagaacgcgc
     6781 cccagatgct tctgtttccc ggcaagctgc cgcacgatgt tctcgaccat cacgacagcg
     6841 ccgtcgatga tcagcccgaa atcgattgcg cccaggctca tcaggttgcc ggagattccg
     6901 aaccggacca ttccagtgag cgcccagagg aacgacaggg ggatggcggc cgagacaatc
     6961 agcgcggcgc gccagttgcc cagcatggcc agcagcaccg ccaccaccag aattgcccct
     7021 tcaaagaggt tcttccgcac cgttgccacg gtggcatcga ccaggtcggc gcggtcgtac
     7081 tgggtgcgca aatcgagacc cggggggaga tgctgctgga tttcgcgcag ccgcgccccg
     7141 aaccgctgcg ccacgatccg gctgttctcc cccgccagca tcatcgccgt gcccagcacc
     7201 gcttcctcgc cattctcggt cgccgcgccc gttcgaaagc tcgagccgac gtcgacatcg
     7261 gccacgtcgc gcaccaggag cggcttgacg gccgcggcga acttgaccgg gaggtcggcg
     7321 atgtcctgtg cggactggac gcgtgcgagc gtgcggacgg tgagttggtc ggggccgttg
     7381 tgaatgacgc cgcccccggc attctcgacg ttctcacgca ccagcgcggc cagatcggca
     7441 aaggtcaatc cggcccgttc gaggtcattc gggcggggct ggacgacgat ctgtttctcg
     7501 tacccgccgt tggcgttgat ttcggctatg ccgggtgtgc cgcgcaacat gggtttcacg
     7561 acgaactcgt gaacctcccg tagttcgcgc agttcctcga tccggtcggc gggacggttg
     7621 gtggcgtccg tgcgaaaggc cagagtgtag tagtaaatct cgcccaggcc ggtgctgatg
     7681 ggcgccagct tcggcgtcaa gcccggtggc aggcggtcga caacggcctg gagccgctcg
     7741 gccaccagtt gacgcgcgcg ataaacgtcc gtgcgatcgg cgaaaatgag cgtgacctgc
     7801 gacagcccga acttggtgag cgaccgcatt tcctgcaccc cctgaatccc cgccaactcc
     7861 atctcgatcg ggatcgtcac ggcctgttcg ctctcctccg gcgcgagggt gatcacctcg
     7921 gtgttgacct gcacctgcgg gctggtgatg tcgggcacgg catcgatcgg caggttgttt
     7981 gccgaccaaa ggccgatgaa gatcgagacg gcggcgacga tgagcaggaa cgcgcgttgg
     8041 cgcagcgaag cgtcgattaa tcggtgcagc atgcagcctc cttagggtct gtgcaaaaat
     8101 tacttccgat ttcgctggag gggatttggc tcgctggcga ggcgcgacga aggagcatag
     8161 ccttgacctc tg
//
LOCUS       DAOQRO010000133         8170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8337_length_8170_cov_4.106716, whole genome shotgun sequence.
ACCESSION   DAOQRO010000133 DAOQRO010000000
VERSION     DAOQRO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_8170_cov_4.106716"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /gene="gltX"
                     /locus_tag="PKK20_06545"
     CDS             <1..1286
                     /gene="gltX"
                     /locus_tag="PKK20_06545"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNU99579.1"
                     /translation="TALFNWLYARHVGGHLILRIEDTDTARNTQAAVDVILHGLRWLG
                     VNWDEGPLTSDATGPYKGDRGPYYQSQRQENYRLRVEALLARDMAYEHEGAVRFRMPR
                     EPIVVPDLIVGEVRRELTDREARDPDFVIVRSDGQPVFHLVNVIDDLEMGITHVIRGE
                     DHLSNTPKHIALFRAFGVAPPVYAHIPLILNPDGSKMSKRDQGASLSTYLEEGYTPEA
                     VVNYLCLLGWSPKDNREILPVREIIDRFDLSQILRHNARFDMAKLQWMNAEYMRAMNP
                     DRFYELCVHALAKAGIDTNRFPLSYVKAALDTCHGKLKTFAELGGYAGFYFTDEISWD
                     TEAAAKGFTAEGRDRLRRLRNAFGALPEFTALHLEACLKNLAGQLGVKVGALVHPARL
                     ACTGKTVGPSLYHLLEVLGRDKVFERVDRALAWNG"
     gene            complement(1299..2288)
                     /locus_tag="PKK20_06550"
     CDS             complement(1299..2288)
                     /locus_tag="PKK20_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNU99580.1"
                     /translation="MTHVERFLAVMSFQAVDRLPRWEWAMWWDQTIARWHREGLPANL
                     HFSQVFDIAAHFGLDPYQQFWFSTTDPTIAATQHHVEGVVATMDDYLRIRPRLFPSHA
                     AAMRSLGPWADRQRRGEAVVWATFEGFFWFSRTLMGFSPLMLAFYDQPELIHAINRDL
                     LAFNLDMLDRMTRLCVPTFMTVAEDLSYNHGPMISKAMFDAFVAPYYRKLIPRLLERG
                     ILVFVDTDGDVTRVVPWLQELGIAGVLPLERQAGVDGMQLRRQFPRFRFVGHFDKMTM
                     TRGEAAMRAEFERLRPLMASGGFIPSVDHQTPPGVSLDQYRLYLRLLDEHTAL"
     gene            complement(2305..4284)
                     /locus_tag="PKK20_06555"
     CDS             complement(2305..4284)
                     /locus_tag="PKK20_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNU99581.1"
                     /translation="MSTLSCKTLLAVAALALGTTFARGQQPSPLVTQTADKSIAVLQS
                     PSSTVKEKTDACRQLAVTGDAAAVPALVALLPDETLNHMARYALETIPDRAVDDALRG
                     ALDRLSGRQLVGVIDSIGVRRDAKSIGALTPRLRATDPDVAQAAARALGSIGTVEAAQ
                     AIEAALGAASAENQIAFCEGLFRSAETLAAKGDNAQALRLYNRLRALPSASHQVRAGA
                     WRGAILTHGLTGLPLLEEAWQSPDHILTAAAARAAMEMRGPEVTRALISLANALNGDK
                     RILALQVLSKRGDPAGLPALIAAVQSNEKPVRLAAIRAMPELGQAAALNPLSLLLGDA
                     DAEIVKTAQEALAALPGDAVDSAVVGMLSSSRASERLTAIDLIGRRRMEACVPALLQA
                     ATDTDASVRPAALKRLGELSSPAQLPHLLDLLLKADAATDRDAAEQAVSAVCARAEAP
                     AACVNQVTERLASAQPDAKGALLRVLTGVGGADALAAVRAAVDDPNAGVRASAIRALG
                     SWKTEDVVPELLALARSSANPTERLLALRGYFGWASNAELPAERRLALCQQAAGVVQQ
                     AEEKKLLLGALGSIKTSGAFAAAVPYLDDAAVRAEAGTAVILIGEELLKGREASEVAS
                     KLVAPLQKAAQSISNPDLARKANALAEQAQKKTAQ"
     gene            complement(4326..5672)
                     /locus_tag="PKK20_06560"
     CDS             complement(4326..5672)
                     /locus_tag="PKK20_06560"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99582.1"
                     /translation="MRTSPSSKPVAAPGPAGLTRRQFVRRAAIAGGVFAAPLLIPSSA
                     LGRDGAVPPSERIILGGIGIGGRGSGVLNWMLPEPDVRFVAVCDPQKQRREATKRMVD
                     NTYGNTDCKVYSNLREFLAERTDIDAVLSTTGDRWHALAAVWAMRAGKDIYSEKPSSM
                     TIAEGQAVVETARRYGRVYQTGTQRLSEANFVFAIELARSGRLGKVHTTYAHIAPWDA
                     AEMRHDWLPGQPEPSKDEVDWDQWLGPCPWRPYNPEYTRGGWRGHYDFHTSCIGEWGA
                     HTFVQAQAGIDMLDTSPVEYEYVNNVSGDGMVTHFANGVKLVLSRGDKYWHGSCGMRF
                     DGPEGWVSVADGYSRPEVSHPSLLADFNKVVQDYMARTQRPMSHVRDFFNCVKSRRLT
                     VAHPVVMHRGMSTVHAANICMWLKRNMKFDPVKEEFVGDAEANRLRSRAMRAPYTV"
     gene            complement(6140..7177)
                     /locus_tag="PKK20_06565"
     CDS             complement(6140..7177)
                     /locus_tag="PKK20_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNU99583.1"
                     /translation="MKCFVTGASGFVGANLAHELAAQGHSLRLLLRPGADLRGLERIA
                     YERIAGDLLDRAALEHGLRGCDWCFHVAACYALWLPDYRPLFAANVEGTRNVLEAAGG
                     AGCSRIVYTSTVGCIGLPRAVGSSLTPSDETAQATRAQMTNPYKESKWEAEAVAHDLA
                     ARGLPIVIVNPTAPVGPRDVKPTPTGKVIVDFLNGRMPAYLDTGLNWVHVRDVARGHV
                     LAAEMGRIGERYILGHAAGNWTMKKALDTLAEITGRPAPRWRIPYGLAAAFAHLDELK
                     SRVTGSPPRAPLAGVRMARHKMFFNPAKAVRELGMPQTPPSQALSDAVQWFEQNGYVN
                     AVRARTLKLAV"
     gene            complement(7231..8010)
                     /locus_tag="PKK20_06570"
     CDS             complement(7231..8010)
                     /locus_tag="PKK20_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HNU99584.1"
                     /translation="MDPEPASPAFVKIIACEIAFREICHLAARTPNVVDLEFLTQGLH
                     DTPAAGCAELQRRIDAVPDGKYDAILIGYGLCSSILNGLRAGPTPLVIPRAHDCITFF
                     LGSKEHYQQCFTERPGTYYYTSGWIECRKRRGNAVQAGFSGFMPAGAAAARQNDFDVW
                     AKKYGEEQARYLMDVMAGWAAHYTHGVLIDFDFTRPLALESQVRQVCDERGWKFEALP
                     GDLRLLERWINGPWTDSEFLVVPPGRQVVPCADDRIVTLAP"
     gene            complement(8014..8148)
                     /locus_tag="PKK20_06575"
     CDS             complement(8014..8148)
                     /locus_tag="PKK20_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNU99585.1"
                     /translation="MAWAKTYEAARVVYIQLGHDHLAYENPSFRRLVANAIRWVAKRD
                     "
BASE COUNT         1448 a         2694 c         2671 g         1357 t
ORIGIN      
        1 gcacagcgct gttcaactgg ctctacgccc gccacgtcgg ggggcatctg attctccgca
       61 tcgaggacac ggacacggcg cgtaacacac aggcggcggt ggacgtcatt ctccacgggc
      121 tccgctggct gggggtgaat tgggacgaag gtccgctgac cagcgacgcg accgggccct
      181 acaaaggcga ccgcggcccg tactatcaat cgcagcggca ggagaactac cggctgcggg
      241 tcgaggcgct gcttgcgcgg gacatggcct acgagcacga gggcgcggtg cgcttccgca
      301 tgccccgcga gccgatcgtc gtgccggacc tgatcgttgg ggaggtgcgt cgcgaactca
      361 ccgaccgcga ggcgcgcgat cccgatttcg tgatcgtccg gtccgacgga cagcccgtgt
      421 ttcatctggt caacgtgatt gacgaccttg aaatgggaat cacgcatgtc attcggggcg
      481 aggatcacct gtcgaacacc ccgaaacaca tcgcgctgtt ccgggccttc ggggtggcgc
      541 ctcccgtcta cgcccacatc ccgctcatcc tcaatcccga cggctccaag atgagcaagc
      601 gcgatcaggg cgcatcgctc tcgacgtatc tcgaggaagg ctacacgccg gaggccgtgg
      661 tcaattacct ctgtctcctg ggctggtctc cgaaggacaa ccgcgagatc ctccccgtcc
      721 gggagatcat cgaccgattc gatctctcgc agattctgcg gcacaacgcg cgcttcgaca
      781 tggccaagct ccagtggatg aatgccgagt acatgcgcgc catgaatccc gaccgctttt
      841 acgagctctg cgtccatgcc ctggccaagg cgggcatcga caccaatcgg ttccccttgt
      901 cctacgtcaa ggccgcgctc gacacctgcc atggaaaact caagaccttc gccgagctgg
      961 gcggttacgc cggcttctac ttcacggacg aaatctcgtg ggacactgag gccgcggcca
     1021 agggtttcac cgctgaaggc cgcgaccgcc tgcgcaggct tcggaatgcg tttggcgcgc
     1081 tgcccgagtt cacggcgctg cacctcgagg cgtgtcttaa gaacctcgcc gggcaactcg
     1141 gcgtcaaggt cggcgccctg gtacatcccg cccgcctggc ctgcaccggc aagaccgtcg
     1201 gaccgagcct gtatcatctg ctcgaagtcc tcggacgcga caaggtcttc gagcgcgttg
     1261 atcgggccct ggcgtggaac ggctgacccg gggccggctc agagcgccgt gtgctcgtcg
     1321 agcaaacgca ggtagagccg gtactggtcc aaggacacgc ctggcggggt ctgatggtcg
     1381 acgctcggga tgaagccgcc ggacgccatg agcgggcgca accgttcgaa ctcggctcgc
     1441 atggcagcct cgccgcgcgt catagtcatt ttgtcgaaat ggccgacgaa ccggaatcgg
     1501 gggaactggc ggcgcaattg cattccatcc acgccggcct ggcgttcgag cgggagcacg
     1561 ccggcaatcc ccaactcttg cagccagggc acgacgcggg tgacatcgcc gtcggtatcc
     1621 acaaacacca ggatgcctcg ctcgagcaga cgcggaatca gcttgcggta atagggcgcc
     1681 acaaaggcgt cgaacattgc cttcgagatc atcggcccgt ggttgtacga gaggtcctcg
     1741 gccaccgtca tgaacgtcgg cacgcagagc cgcgtcatcc gatccaacat atccaggttg
     1801 aaggcgagca gatcgcggtt gatggcatga atcaactccg gctggtcgta gaacgccagc
     1861 atcaggggac tgaatcccat gagcgtgcgc gaaaaccaga agaaaccctc gaacgtcgcc
     1921 cacacaaccg cttcgccccg gcgctgccga tccgcccacg gccccagact ccgcatcgcc
     1981 gccgcatgcg atgggaagag cctgggccga atccgaaggt agtcgtccat cgtggccacc
     2041 acaccctcga cgtggtgttg cgtggcggcg atcgtcgggt cggtcgtgct gaaccagaac
     2101 tgctggtacg gatcgaggcc gaagtgcgcg gcgatgtcga agacctggct gaagtggaga
     2161 tttgcaggca ggccttcccg atgccagcgc gcgatggtct ggtcccacca catggcccat
     2221 tcccagcgcg gcaggcggtc caccgcctgg aaactcatca ccgccagaaa gcgctcgaca
     2281 tgcgtcatca atgactgaga gcccctactg cgccgtcttt ttctgcgcct gctcggccag
     2341 agcgttggcc ttgcgcgcca gatcgggatt cgagatggat tgcgcggcct tttgcagcgg
     2401 cgccaccagc ttcgacgcca cctcggacgc ctcacgcccc ttgagcaact cctcgccgat
     2461 gaggatcacc gccgtgccgg cttccgcccg caccgccgca tcgtcgagat aaggcacggc
     2521 cgcagcgaaa gcgccggacg tcttaatgct ccccagcgcg cccaagagca gcttcttctc
     2581 ctcggcctgc tggacaacgc ccgcggcctg ctggcacaaa gccagacggc gctcggcggg
     2641 caattcggcg ttggatgccc acccgaagta gccccgcaag gcgagaagcc tctcggtcgg
     2701 gttggcggag gagcgggcca gggcgagcaa ttcggggacg acatcctcgg tcttccatga
     2761 acccagcgcc cggatcgcgg aggcgcgcac tccggcattg ggatcgtcca ccgcggcccg
     2821 aaccgccgcg agagcgtcag ctccacccac accggtcaga acgcgcagca gggcgccttt
     2881 ggcgtcgggc tgagccgagg ccagtcgttc cgtgacctgg ttcacacacg cggccggcgc
     2941 ctcggcgcgc gcgcacacgg cgctcacggc ctgttcggcg gcatcgcgat ccgtggcagc
     3001 gtcggccttg aggagcaaat cgagcagatg aggcaactgg gccggactgc tcagttctcc
     3061 aaggcgtttc aaggccgccg gacgcaccga ggcatcagtg tcggtcgcgg cctgcaacag
     3121 ggccggcacg cacgcctcca tgcggcggcg tccgatcaga tcgatcgcag tcaaccgctc
     3181 cgacgcgcgc gaactggaca gcattccgac gactgcgctg tccacggcgt cgccgggcag
     3241 cgccgcgagg gcttcctggg ccgtcttgac aatctccgca tcggcatcgc cgagcaatag
     3301 gctcagcgga ttcagcgccg cagcctgacc aagctcgggc atggcgcgaa tggccgcgag
     3361 gcgaacgggt ttctcgttgc tctggacggc cgcaatcagc gcgggcaatc cggccggatc
     3421 gccgcgtttg ctcaagacct gcagggcgag gattcgcttg tcgccgttca gcgcattggc
     3481 cagcgagatc agggcacgcg tgacctcggg cccgcgcatc tccatcgcgg cccgggcggc
     3541 agcggcggtg aggatgtgat ccgggctttg ccaggcctct tcgaggagag gaagacccgt
     3601 caggccgtgg gtcaggatcg caccgcgcca tgcgcccgct cgaacctgat gcgaagcgct
     3661 cggaagcgcg cgcaggcggt tgtacaggcg cagggcctgg gcgttgtcgc ccttggcggc
     3721 gagggtctcg gccgaccgga acaggccctc gcaaaaggcg atctggttct ccgcggacgc
     3781 ggcccccagt gcggcctcga tggcctgggc tgcctcgacg gtgccaatgc tgcccagggc
     3841 ccgggcggcg gcctgcgcga catcgggatc cgtcgcgcgc agtcgcggcg tcaaggctcc
     3901 aatcgacttc gcgtcgcgcc gcacgccgat gctgtcgatg acgccgacga gttggcgtcc
     3961 gctcagtcgg tcgagcgcgc cgcgcagggc gtcgtcgacg gcgcggtcag gaatggtttc
     4021 gagggcgtac cgggccatgt gattgagcgt ttcatccggc aggagcgcca ccagggccgg
     4081 cacggcggcg gcgtcgccgg tcaccgccaa ttggcggcag gcatcggtct tctccttaac
     4141 ggtcgaactg ggggactgga gcaccgcaat cgacttgtcg gccgtttgcg tgaccagcgg
     4201 ggacggttgc tggccgcggg cgaacgtcgt gccgagggca agcgccgcaa ctgccaggag
     4261 ggttttgcat gagagagtgg acataatcgt gtatctttcg tgtcgcttca gatggtcgga
     4321 gagattcaga cagtgtaagg tgcgcgcatg gccctcgagc gcaatcggtt cgcttccgca
     4381 tccccgacga actcctcctt gacgggatcg aacttcatgt tgcgcttgag ccacatgcag
     4441 atgttcgcgg catggacggt cgacatgccc cggtgcatca ccaccgggtg agccacagtg
     4501 agccgccgcg acttgacgca attgaaaaag tcgcgaacgt ggctcatcgg ccgctgcgtc
     4561 cgcgccatgt agtcctgcac gaccttgttg aaatcggcga gcaggctcgg atgcgacacc
     4621 tcgggccgcg aatacccgtc ggcgaccgac acccaccctt caggcccgtc aaaacgcatc
     4681 ccgcacgagc catgccaata cttgtcgccg cgcgacagga ccaatttcac gccgttggcg
     4741 aagtgggtga ccatgccgtc gccggagacg ttgttgacgt attcgtattc gaccggcgat
     4801 gtgtcgagca tgtcgattcc ggcctgggcc tggacgaagg tatgggcgcc ccattcgccg
     4861 atgcaactgg tgtggaaatc gtagtgcccg cgccagcccc cccgggtgta ctcgggattg
     4921 tacgggcgcc acggacacgg ccccagccat tgatcccagt ccacttcgtc tttggacggc
     4981 tccggttgcc cgggcagcca gtcgtgacgc atctccgccg catcccaggg cgcgatgtgc
     5041 gcgtacgtcg tgtgcacctt gcccaagcgc ccgctgcggg ccaactcgat tgcaaagacg
     5101 aagttcgcct cgctcagacg ctgggtgcca gtctgataaa cccgtccgta acggcgcgcg
     5161 gtctcgacca cggcctgccc ctccgcaatc gtcatcgagg agggcttttc cgagtagatg
     5221 tccttgcccg cccgcatagc ccagacggcg gccagcgcgt gccaacgatc gcccgtggtg
     5281 ctcagcaccg cgtcaatatc cgtccgctcg gcaagaaact cgcgcagatt cgagtacacc
     5341 ttgcagtccg tgttgccata cgtgttgtcg accatccgct tggtcgcctc gcgccgctgc
     5401 ttctgcgggt cgcagaccgc cacgaaccgc acatcgggct cgggcagcat ccagttgagc
     5461 acgcccgaac cgcgcccccc aatgccgatg ccgcccagaa tgatgcgttc gctcgggggc
     5521 accgcgccat cccgcccgag tgccgacgac ggaatgagca ggggagccgc gaacacgccg
     5581 cccgcgatgg cggcgcgcct cacgaattgg cggcgtgtaa ggcccgccgg gccgggggcg
     5641 gcgacgggtt tcgaggaggg ggaagtgcgc atggaggtcg agacagatgg ttcgtgcagt
     5701 gccataagga cggcacccta gcaagggcgc ccgcgctcga aaagccctta gtcgcgaaaa
     5761 attgccggag ggaacaaaca gactgccgat tcaaggttga ccatctttcg agagttggct
     5821 gcaaatcgtc ggcattcagg ccgattggcc gatctctctg aaccaatggt gcgagccaag
     5881 atcaaggaag cccaggccgt cacccagttg aacggcgccc tgaaggaact gggcatcgac
     5941 gggcggttca ggaaccatgg caactgacat cgggctgctc agtgaatgcg gtaactcatg
     6001 ggaacacggg tggtggcggt tgctgacatc acaccgcatt gagctctggt gaagggacag
     6061 agacgcagca actgcacaag tctcccagcc gcgtttcgcg ctactccacg cgaacgaagc
     6121 ggcagtcgca cctctccagt cacacggcca acttcagagt ccgggcgcga acagcattga
     6181 cgtacccgtt ttgctcgaac cattgcaccg cgtccgacag ggcctgtgac ggcggcgttt
     6241 gaggcattcc caactcgcgg acggccttgg ccggattgaa gaacatcttg tgccgcgcca
     6301 tgcgcacccc ggccaagggg gcgcgcggcg gactgccggt cacgcgcgac ttgagttcat
     6361 cgaggtgagc gaatgccgcc gccaaaccgt aggggatgcg ccagcgcggc gcgggccgcc
     6421 cggtgatctc ggccaacgtg tcgagggcct tcttcattgt ccaattcccg gcggcatggc
     6481 cgaggatata gcgttcgccg atccgtccca tctcggcggc gagcacgtgg cctcgagcca
     6541 cgtcgcgcac gtggacccag ttgagtccgg tatcgagata ggccggcatg cgtccgttga
     6601 gaaagtcgac aatcaccttg ccggtggggg taggcttgac gtcgcggggg ccgaccggcg
     6661 cggtcgggtt cacgatcacg atcggcaggc cccgcgccgc caggtcgtgc gcgacggcct
     6721 ccgcctccca tttggattcc ttgtagggat tggtcatctg ggcgcgggtg gcctgggcgg
     6781 tctcgtcgct cggggtcaga ctcgagccga ccgcgcgggg gagtccgata cagcccaccg
     6841 tgctggtgta gacgatgcgg gaacaaccgg ccccgcctgc ggcttcgagc acattgcgcg
     6901 tgccctcgac atttgcggca aacagcgggc gatagtcggg gagccagagc gcatagcatg
     6961 ccgccacgtg gaagcaccag tcacagccgc gcagcccgtg ttcgagcgcc gcccgatcga
     7021 gcaggtcgcc cgcgatccgc tcatatgcaa tccgttcgag tccgcgcaga tcggcgcctg
     7081 gacgcagcaa cagccgcaac gaatggccct gagccgccaa ctcatgcgcc agattcgcgc
     7141 caacgaaccc ggacgcgccg gtcacaaagc acttcacgcc tgtccttatg agggcggagc
     7201 gccccccctt tcaacctcaa aaccggccgc tcacggagcg agggtcacga tccggtcgtc
     7261 ggcgcagggc acgacctggc ggccgggcgg aacgacgagg aactccgagt ccgtccacgg
     7321 gccgttgatc cagcgttcga gcaggcggag atcgccgggc aatgcctcga atttccagcc
     7381 gcgctcgtcg cacacctgcc gcacctgcga ctcgagcgcg aggggcctgg tgaagtcgaa
     7441 gtcgatcagc acgccgtgcg tgtagtgggc cgcccatccg gccatgacgt ccatcaagta
     7501 ccgcgcctgt tcctcgccgt acttcttcgc ccacacatcg aagtcgtttt ggcgggcagc
     7561 cgcggcgccg gccggcatga agccgctgaa acccgcctgg accgcattgc cgcgccgctt
     7621 gcgacattcg atccagccgg aggtgtagta atacgtcccg ggacgttcgg tgaaacactg
     7681 ctggtaatgc tccttcgatc ccaggaagaa tgtgatgcaa tcgtgagcgc gcgggatgac
     7741 gagcggcgtt gggccggcgc ggagtccgtt gaggatgctg ctgcataggc cgtagccgat
     7801 gaggatggcg tcgtacttgc cgtccgggac ggcatcgatc cgccgctgga gttcggcgca
     7861 gccggccgcg ggcgtgtcat gcagcccctg cgtcagaaac tcgaggtcga cgacgttcgg
     7921 cgtgcgggcg gcgaggtggc agatttcgcg aaaagcgatc tcgcaggcga tgatcttgac
     7981 gaacgcggga gaggccggct ccgggtccat gtgtcagtcc cgtttggcga cccacctgat
     8041 ggcgttggcg accaaccggc ggaaactcgg gttctcgtat gcaaggtggt cgtgtcccag
     8101 ttgaatgtag acgacccgcg ccgcctcgta ggtcttggcc caggcgatgg tcttgccgct
     8161 ggccggctcc
//
LOCUS       DAOQRO010000134         8090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8460_length_8090_cov_3.621655, whole genome shotgun sequence.
ACCESSION   DAOQRO010000134 DAOQRO010000000
VERSION     DAOQRO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8460_length_8090_cov_3.621655"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PKK20_06580"
     CDS             complement(<1..1419)
                     /locus_tag="PKK20_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99586.1"
                     /translation="MFSLLRAKRRPVLWCLAAFVPWGVHARSSLVALGEEYAVGGSMP
                     GDQVFARAAVGPNGGYLVWHDNVMDGAGYGIGARRLDQNFVAGLGAFRVNKTVAGDQE
                     HPQVAMFSDGAAIVVWQGGAVGFPDVFARYLAADGTFATGDIRVNSNSYTQDRQERPQ
                     VAVLKGDTAVVVWASYGQDGSHYGVYGQRLDARGKKIGGEFLVNETVFLSQRSPSVAA
                     MPDGRFVVVWVSEKQNAAGEPVAGQEGVATVGPGRVVQPYDVSLFGRVFDASGPLGSE
                     FKINTSAYVCANPVVSVDALGGFLVAWSSHVGRVEVSGVTSARGWDIRSRAFAPDGTP
                     SGEETELNERTFGDQFLPSAASVGSNHLVAWISLGQDGSREGVYGRVVAENGAPLDAE
                     FRVNSFTPGAQKLPAVVSNGKGRAFVVWSRARGGLASFDLYAQRYASDAELVAPAKPM
                     VLALSASKLSVTWPSLENLGAVS"
     gene            complement(1653..2564)
                     /locus_tag="PKK20_06585"
     CDS             complement(1653..2564)
                     /locus_tag="PKK20_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNU99587.1"
                     /translation="MTAPGAHAPRPSLLLVDGHAYAYRSFHAIRNLSSPSGHPTNAIF
                     GFVKALKTLESSLAPTHLAVVWDGGLAADRLALLPEYKAQRPPMPDALEAQLDGMVSW
                     LAASGIFSLCRDGVEADDAIASLASHAAGSGVDVVIASADKDFLQLVSPRVGIINPND
                     KTGRIWSAEDVVAKTGVRPEQIVDWLCLVGDAVDNIPGIPGVGAKTAAELLRQFGSCR
                     RLLEQVENITSDRIRTSLVEGASNLLRNREIIRLKDDLDLQVALDHLKASPPDRGRLA
                     TLYRQWGFRSLLAETEAADSAQGTLDL"
     gene            complement(2734..3321)
                     /locus_tag="PKK20_06590"
     CDS             complement(2734..3321)
                     /locus_tag="PKK20_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNU99588.1"
                     /translation="MYTRHILFAALALCGLEGAFEMHAQVVNQPQGFETVQPTTVPVP
                     LGPTLDVLPHVSADGYTIQMNLIPTMTEFLGYEQTPYTAQVGTIETLPYPLPSFRVRQ
                     VTTTAIVWDGQTIMLGGLISEKVNKVKDKVPVLGDLPLFGRLFRSESSSTQKKNLVIF
                     VTPTIIDPAGNRIHTEDNLPYDPNRIPAQVPFAPQ"
     gene            complement(3357..5726)
                     /locus_tag="PKK20_06595"
     CDS             complement(3357..5726)
                     /locus_tag="PKK20_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99589.1"
                     /translation="MIRSALTLLILALLVGSAPSAFAQVAPADPVTEEAVRRQEMSII
                     LRKTLADAQAEQKLGDYGTAAKLYEEAWGYVQKIGEKVIEKEHRETVQGFSHVYLELA
                     KRSFSRGQYNEARSQVSRVLRIDPKNRDALNLAARVDKALRDQAGRVPSKETLALMPE
                     VQEEKTTAATLVQDGKLLYEMGKLEEAAAKLRAAVKLDPENQGAFYYARLVEEAVYSI
                     EARKREAMAKSKMVEVERAWNPPVKWGDITNTTNPFARTNTIYTGAGRRVIFSKLEKI
                     RFNQYPVSGDMPGVPLSVVVKDLDEEVKQRDPDKKGLNFILAPYVDIQVPSALPMVPG
                     QIDPMTGQPIGTAPQQEQFDLGSDVLIKIEPPLKDVSLKDVLDTIIKVSNKQIKYSVE
                     EYAIVFTRKIPEQEQLFTRQFRVNPNTFVQGLEGVIGEQFVGASIGSDSSGTGTTTGG
                     MGGGMMGGMGGGYGGGYGGGGGFIIPRVDVTGYGGMGGMGGMGGGMMGGMGGMGGMGG
                     YGGGSPMGYMSTGGSLMGVNRMTDMSVVNQMVVAYFSAAGVNLGGSNLLAGGGMMGGM
                     GGGFTGGGFQNAAGKSVFYNDRLGLLLVRATLQDLDIIEAAIQVLNLTPDQITIEAKF
                     VEIGQDDAKALGFDWLLGNFLMRGGAIGASGGTYPSVAGEPSPANPSGVFPGVFGIPS
                     ALPNAGTDQIITKGLRNSDAEGSPIPALGTITGILTDPQFRVVIRALEQRSGVDMLAA
                     PKVTTVSGRQAQIQSVDIRQIVTGLNVGASGTGGTISTTGAGTTGTVTQ"
     gene            complement(5770..6561)
                     /locus_tag="PKK20_06600"
     CDS             complement(5770..6561)
                     /locus_tag="PKK20_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99590.1"
                     /translation="MEFLKKHYEKLILSVVLLGMAIAAALLPLKVSQVRQELQQATLN
                     IERARIKPLPELDLSTNNALLRRVRTPGRLTLDAPGHNLFNPIQWKKKPDGTPVPSEQ
                     FGLSALVVTNITPLYLRIQFRGVRDYGSDVRYDFMVTREAATNSAARGPLPNTLAVGA
                     RTDLFTVRDVDGPKENPQGLILELADSRQPVVVTRDKPYTEVQGYMADLWHKTENKVF
                     LRQRQGQKISFGGETYIIIAISESDVTLRDSRTQKQTTISLKAAP"
     gene            complement(6574..7704)
                     /locus_tag="PKK20_06605"
     CDS             complement(6574..7704)
                     /locus_tag="PKK20_06605"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HNU99591.1"
                     /translation="MDWVKKNLLLVVSGAVTLVLLGVAGYFLWSKNALEARVTADLAM
                     QTSELNQLSNSDPHPGNEKVKNIEAAKEQDKKLQEFVAEARKTFVPIDYPTNLDSGQL
                     KLLLDTVIDVLHRKADGMGVKLHTDPKYAFTFGAQRTQMYFDQKDILPTARMMIDIKT
                     IATMLFDARILALDGIRRCTVSSLDTPGPEFWNKKPTTNDTYGAVLTPYEFTFHCFTP
                     ELNRVVETLYRSPHAFLLKSVVVDTSPSQLLERPADGSLDPMGPMMMPSMNLAQLQMM
                     LRYGGRGMMRYATPPPQEVPPPTARGGLTPLLDEKPFRAVLWVESIRLLDPEEIKAAK
                     AAARTSRPVRTVTPEGEEPGAAPTATGGETPAEPAPDAAPVP"
     gene            complement(7706..>8090)
                     /locus_tag="PKK20_06610"
     CDS             complement(7706..>8090)
                     /locus_tag="PKK20_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99592.1"
                     /translation="PMMDIRMLMRYGLLPRGLKLTTEGGEKMDLASALASAQRGSSET
                     QTKQATTNEISVINLTCRGLSWNKLFATADNELAYTLLRELEANSQFMAGTNGTQLAG
                     QMEQDETTSTFKFDVKLKLAKPIKF"
BASE COUNT         1508 a         2500 c         2494 g         1588 t
ORIGIN      
        1 ggacaccgcc cccagattct cgagcgaggg ccaggtcacg ctgagtttgg acgcgctgag
       61 tgcaagcacc atcggcttgg cgggggcaac taactcggcg tcgctggcgt atctctgggc
      121 gtacaagtcg aagctggcca gccccccacg cgctcgggac cagaccacga aagcccgtcc
      181 tttgccgttc gagacgaccg ctgggagttt ctgagctccg ggagtgaagc tgttaacccg
      241 gaactcggca tcgagcgggg cgccattctc ggcgacgacc cgcccgtaga caccctccct
      301 ggacccatcc tggccgaggc tgatccaggc tactaaatga ttgctgccca cgctggcggc
      361 gcttggcaga aactgatcgc cgaaagtacg ctcgttcaac tccgtctcct ctccggacgg
      421 agtgccatcc ggggcgaaag cccgcgaccg gatgtcccaa ccgcgcgcgc tggtgactcc
      481 actgacttcg actcggccga catgcgaact ccaggccacg aggaaaccgc cgagggcatc
      541 gacgctgaca acgggattcg cgcagacata ggcggaggtg ttgattttga actcggagcc
      601 cagcggcccc gaggcgtcga agacacgtcc aaagaggctg acatcgtaag gctgcacgac
      661 ccggccgggg ccgacggtgg cgaccccctc ctggccggcg accggctccc cggccgcatt
      721 ttgtttctcg gagacccaga caacgacgaa ccgtccgtcc ggcatcgccg caacggacgg
      781 actccgctgg ctgaggaaaa cagtctcgtt cacaaggaac tcgccgccga tcttcttgcc
      841 tcgcgcgtcg agccgctgtc cgtacacgcc gtagtggctg ccgtcctggc cgtagctggc
      901 ccacacaacc accgcggtat cgcctttgag aacggcgacc tggggacgct cctggcgatc
      961 ctgggtatag gaattggaat tcacccggat atcgccggtg gcaaacgtcc catccgcggc
     1021 cagataacgc gcgaagacat cggggaagcc gaccgcaccg ccctgccaga caacgatcgc
     1081 ggcgccgtcg gagaacatgg cgacctgcgg atgctcctga tcgccggcga cggtcttatt
     1141 gacgcggaag gcgccaagcc cagccacgaa gttttgatcc aggcgacggg cgccgatccc
     1201 atagccggct ccgtccatga cgttgtcatg ccagaccaga tagcctccgt tgggcccgac
     1261 cgccgcgcgt gcgaacacct gatcgcccgg catggagcca cccacggcgt attcctcacc
     1321 cagcgcgacg agggaggacc gggcatgaac gccccaggga acgaaggcag ccagacacca
     1381 caaaacgggc cgccgtttgg cacgcaacaa cgaaaacata aagtcacggc tttctagcaa
     1441 agcacacttt ctttgtccac gctttcttcc gaaaaactga cttcaggcca actggcagca
     1501 caacccttgc cagagttggg ccgcgctgcc atgggcattc ggttttcagg cagtctcaaa
     1561 cccgcagagc agacacctgc aatatccgac tgcccagtgt gctgacgcag tgcaacgatg
     1621 cgggttaggc cgatcgaatc ggccagccgc tttcatagat ccaaagtccc ttgggccgaa
     1681 tccgccgcct cggtctcggc gagcaacgag cgaaaacccc actgccgata cagcgtggcg
     1741 aggcggcccc ggtcgggcgg cgacgccttc aagtggtcca gtgcgacctg caaatccaaa
     1801 tcgtctttca accgaatgat ctcccgattt cgaagcaaat tggacgcccc ttcgaccagg
     1861 ctggtccgga tccgatccga cgtgatgttt tcgacttgtt cgaggaggcg gcggcaactg
     1921 ccgaattggc ggagcagttc ggcggctgtt ttcgctccga cacccgggat gcccgggata
     1981 ttatccacgg cgtcgccgac aaggcagagc caatcgacga tttgctcggg ccgcaccccg
     2041 gtcttggcga cgacatcctc ggccgaccag atgcgaccgg tcttatcatt cgggttgatg
     2101 atgccgacgc gcggcgaaac caactgcaga aagtccttgt ccgcgctggc gatcacaacg
     2161 tcgacccccg aacctgcagc gtggctggcc agggacgcga ttgcgtcgtc ggcctcgaca
     2221 ccgtcccggc agagcgagaa tatcccagac gccgccagcc aggacaccat gccgtcgagc
     2281 tgcgcctcga gagcgtcggg catgggcggg cgttgagcct tgtactcggg cagcaaagca
     2341 agccgatcgg ctgcaagccc gccatcccag acgacggcca ggtgcgtggg cgcgaggctc
     2401 gattcgaggg tcttcagggc cttgacgaat ccgaagatgg cgttggtggg gtgtccggaa
     2461 ggcgatgaca ggttgcggat tgcgtggaag gagcgatagg catacgcatg tccgtccacg
     2521 agcaacagtg agggccgggg ggcatgggcg ccaggcgcgg tcatccgaaa aagagcggcg
     2581 gacgggcgtt aagtcctgtt cgcgcctggg agacgcgtga ccggaggcaa tcggtcgcca
     2641 tgattcgcac cacaccacta cgagagtcca acgttcgaaa tcggaaatcc ctgatctcaa
     2701 acttggaatc gacaccctgc cccgaggcgc cggtcactgc ggcgcgaatg gcacctgggc
     2761 tgggattctg ttgggatcgt aggggaggtt gtcttccgtg tgaattcggt tgccggcggg
     2821 atcgatgatc gtcggagtga cgaagatgac cagattcttc ttctgcgtgg acgagctttc
     2881 gcttcggaaa agacgtccga acaatggcag gtcacccagg acgggcacct tgtccttgac
     2941 cttgttgact ttttcgctga tcagaccgcc gagcatgatg gtctgcccgt cccaaacaat
     3001 cgccgtggtg gtaacctgtc ggacccggaa ggatggcagc gggtacggca gtgtttcgat
     3061 ggtccccacc tgtgcggtgt agggggtttg ttcgtatccc aggaattccg tcatcgtcgg
     3121 aatcaggttc atctgaatcg tgtacccatc ggccgaaacg tggggcaaca catccagagt
     3181 gggaccaagc ggaaccggga cggtcgtggg ttgaaccgtc tcgaaacctt ggggctgatt
     3241 caccacctga gcgtgcattt caaatgcgcc ttccaacccg cagagggcca atgcggcgaa
     3301 cagaatgtgt ctcgtgtaca tgtgcatatg gacagtagaa ttggagaatg aagtgcctat
     3361 tgagtgacgg tgccggtggt gcctgcgccg gtggtgctga tggtgcctcc ggttccggaa
     3421 gcgcctacgt tgagcccagt cacaatctga cggatgtcga cactctgaat ctgcgcttgc
     3481 cgcccgctca cagtcgtcac cttgggcgcg gccaacatgt cgacgccgga tcgctgttcg
     3541 agcgcccgga tcaccacgcg gaattgcgga tcggtcagaa tgcccgtgat ggtgccgagc
     3601 gccggtatgg gcgaaccttc ggcatcgctg ttgcgcaggc ccttggtaat aatctggtcc
     3661 gtcccggcat tgggaagggc cgaaggtatc ccgaagactc ctgggaagac gcccgacggg
     3721 ttggccggcg agggctcgcc agcaacggag ggataggtgc caccagaagc cccaatggct
     3781 cctcccctca tcaggaagtt gcccagaagc caatcgaacc cgagggcctt cgcgtcgtcc
     3841 tgcccaatct cgacgaactt cgcctcgatc gtgatctgat cgggcgtgag gttgaggacc
     3901 tggatggccg cttcgatgat gtccagatcc tggagcgtgg cacggaccag gaggagcccg
     3961 aggcggtcat tgtaaaagac cgacttgccc gccgcgttct gaaagccgcc gccggtgaat
     4021 ccgccgccca tgccgcccat cattccgccg ccagccagca ggttcgagcc gcccaggttc
     4081 acgcctgcag cgctgaagta ggcgacgacc atctggttga ccacggacat gtcggtcatg
     4141 cggttgacac ccatgagcga accgccagtg gacatgtagc ccattggcga gccgccgccg
     4201 tagccgccca tgccgcccat cccgcccatg ccgcccatca ttccgccgcc catgccgccc
     4261 atgccaccca tgccgccata accggtgacg tcgacgcgcg gaatgatgaa cccgcctccg
     4321 ccgccatagc caccgccgta gccaccgccc atcccgccca tcattccgcc gcccataccg
     4381 cccgtggttg ttccggtgcc gctggagtcc gagccgatgg aggccccgac gaactgctcg
     4441 ccgatcacgc cttcgaggcc ctgcacgaac gtgttcgggt ttacgcggaa ctggcgggta
     4501 aagagctgtt cctgctcggg aatcttgcgc gtgaacacga tcgcgtactc ttcgaccgag
     4561 tatttgatct gcttgttcga caccttgatg atcgtatcga gcacgtcctt gaggctcacg
     4621 tctttcaagg gaggctcgat cttgatcaag acatcggatc ccaggtcgaa ctgctcctgc
     4681 tggggcgccg tgccgatcgg ttgaccggtc attggatcga tctgcccggg caccatcggc
     4741 agggccgaag ggacctgaat gtcaacgtac ggagccagga tgaagttgag gcctttcttg
     4801 tcgggatcgc gctgcttcac ctcctcatcc aaatccttca ccaccacgct cagcgggacg
     4861 ccgggcatat cgccgctcac ggggtactga ttaaaccgaa tcttctcgag cttgctgaag
     4921 atgacgcgcc gaccggcccc ggtatagatc gtgttcgtgc gggcgaaggg gttcgtggta
     4981 ttcgtgatgt cgccccattt caccggcgga ttccatgcgc gctcaacctc gaccatcttc
     5041 gacttcgcca tcgcctcgcg cttgcgcgcc tcgatcgaat acacagcctc ctcgaccaat
     5101 cgcgcgtagt agaacgcgcc ctggttttcg ggatcgagct tcacggccgc ccgcaacttc
     5161 gcggcggcct cttcaagctt ccccatctca tagagcaact tgccatcctg cacgagagtg
     5221 gccgccgtgg tcttttcctc ttgcacctcg ggcataagcg ccagggtctc cttgctggga
     5281 acccgccctg cctgatctct gagggccttg tcaactctcg ccgcgaggtt cagggcgtcc
     5341 cggttcttcg gatcaatccg cagcacgcgg gagacctggc tcctggcttc gttatactgt
     5401 ccgcgggaga aggatctttt cgcgagttcg aggtagacgt ggctgaaacc ctggaccgtc
     5461 tcgcgatgct ctttttcgat gaccttctcg ccgattttct ggacgtagcc ccaggcctcc
     5521 tcgtaaagct tggccgccgt tccgtagtcg ccgagtttct gctcggcttg ggcgtcggcc
     5581 agcgtcttgc ggaggatgat gctcatttcc tgccttcgca ccgcctcctc cgtgacggga
     5641 tccgccggag cgacttgagc gaacgcggaa ggcgcgctgc caaccagcag cgcgaggatg
     5701 aggagggtaa gagcggaacg gatcatggat ttctcttcag ttgggacagg aaacataaag
     5761 tcgcattgcc tacggcgcag ctttgaggct gatggttgtc tgcttttgag tccggctgtc
     5821 tcggagtgta acgtcgctct cgctgatggc aataataatg taggtctcgc cgccgaagga
     5881 aatcttctgt ccctgacgct gtcgaaggaa gaccttgttt tcggtcttgt gccacaaatc
     5941 ggccatgtaa ccctgcacct cggtgtacgg tttgtcgcga gtcacgacca ccggctgccg
     6001 cgagtcggcc agttcgagaa tgagcccctg cggattctcc ttcggtccat cgacgtccct
     6061 gacggtaaag aggtcggttc tggcgcccac ggcgagcgtg ttgggcaatg ggccgcgcgc
     6121 cgcgctgttg gtggcggcct cgcgtgtgac catgaagtca tagcgaacgt cgctgccgta
     6181 atcgcgcacg ccccggaact ggattctcag atagagcggc gtgatgttcg tcaccaccag
     6241 agcgcttaat ccgaactgct cgctgggcac cggagtgccg tccggcttct tcttccactg
     6301 gatggggttg aacaggttgt gaccgggagc atcgagggtc agtcggcccg gggtacggac
     6361 ccgtcgcaag agggcgttgt tggtggagag gtccaactcc gggagaggct tgatgcgggc
     6421 ccgttcgata ttgagggtcg cctgctggag ttcttgtcgc acctggctga ccttgagcgg
     6481 caggagcgcg gcggcgatgg ccattcccag caacaccacg ctgagaatca gcttctcgta
     6541 atgcttctta agaaactcca tggtggaatg cggctaagga acgggggcag cgtctggagc
     6601 gggttcggca ggggtctcgc ccccagttgc ggtcggggcc gcgcccggct cttccccttc
     6661 gggagtcacg gtgcggacgg gccggcttgt gcgcgcggcc gcctttgctg ccttgatttc
     6721 ctcgggatcc agaagacgaa tcgactcaac ccacagaacg gcgcggaacg gcttctcgtc
     6781 gagcaagggg gtcaaaccgc cacgggctgt gggcggaggc acctcctggg gtggcggagt
     6841 ggcatagcgc atcatgccgc gccccccgta gcgcagcatc atctgcagtt gcgccagatt
     6901 catgctgggc atcatcatgg gacccatcgg atcgagtgag ccgtcggccg gtctttcgag
     6961 cagttgagag ggcgatgtgt ccaccacaac actcttgaga agaaaggcgt gcggactgcg
     7021 gtagagagtt tcgaccacgc ggttgagttc aggagtgaag caatggaacg tgaactcgta
     7081 cggggtcagc acggcgccgt acgtgtcgtt ggtcgtcggc ttcttgttcc agaactccgg
     7141 accgggagtg tccaggctgc tgaccgtgca acggcgaatc ccatcgagag cgaggatgcg
     7201 ggcgtcgaag agcatcgtgg cgatcgtctt gatgtcgatc atcatgcggg cggtcgggag
     7261 gatatccttc tggtcgaagt acatctgtgt gcgttgcgcg ccgaacgtga acgcgtattt
     7321 cgggtctgtg tgcagcttca cacccatgcc gtccgccttc cggtgcaata cgtcgatgac
     7381 ggtatcgagc agaagcttca actgaccgct gtcgagattg gtaggataat cgatggggac
     7441 gaacgtcttg cgggcctcgg ccacgaattc ctggagcttc ttgtcctgct cctttgcggc
     7501 ttcgatattc ttgactttct cgtttccggg gtggggatcc gagttggaca actggttcaa
     7561 ctcgctggtc tgcatcgcga ggtctgctgt gacccgcgcc tcgagcgcgt tcttggacca
     7621 caggaaatac cccgcaacgc ccaggagcac gagcgtcacg gccccgctca cgaccagcag
     7681 caaattcttc ttgacccagt ccatattaga atttgatggg cttcgcgagc ttcagtttga
     7741 cgtcgaactt gaacgtgctg gtggtctcgt cctgttccat ctgcccagcc aattgcgtcc
     7801 cgttggttcc agccatgaac tgcgagttgg cttccaactc cctgagcagc gtgtaggcca
     7861 gctcgttgtc agccgtcgca aacagcttgt tccagctcag tccgcgacag gtaagattga
     7921 tcactgaaat ctcgttggtt gtggcctgtt tggtctgggt ttcggagctc ccgcgctgtg
     7981 cggaggccag agcggacgcg agatccatct tctccccgcc ttcggttgta agcttcaacc
     8041 ccctgggcag caacccgtag cgcatgagca tgcggatgtc catcatcggg
//
LOCUS       DAOQRO010000135         8033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8560_length_8033_cov_4.799198, whole genome shotgun sequence.
ACCESSION   DAOQRO010000135 DAOQRO010000000
VERSION     DAOQRO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8560_length_8033_cov_4.799198"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..538
                     /locus_tag="PKK20_06615"
     CDS             341..538
                     /locus_tag="PKK20_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99593.1"
                     /translation="MQAYKNAGCGGPTLYRHAEGAGLAFYDGRAQRLRKEKVWIAADK
                     NANPKRPGMWVADLETWRRNH"
     gene            558..692
                     /locus_tag="PKK20_06620"
     CDS             558..692
                     /locus_tag="PKK20_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99594.1"
                     /translation="MESISAAIRGSPIAGEEHSDRCVRTAIGAKAYPKPSEDITLIED
                     "
     gene            700..3585
                     /locus_tag="PKK20_06625"
     CDS             700..3585
                     /locus_tag="PKK20_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNU99595.1"
                     /translation="MKLCIFSGLILTALCAAAAEFHVSPGGDDANAGTRDKPFATPAR
                     AQQAARAAAGKEPVTVWLRGGTYALPSPLVFTPADSGTPDAPVVWQAFPGESPVISGG
                     LKLDLEWEPHRNGILKARVPAGLTSDQLFVNGRRQLMARYPNYDASVRPYGGYAADAF
                     GAERAARWADPAGGYIHAMHSAHWGGYHYRITGRNEKGEIIYEGGWQNNRQMGMHAQH
                     RYVENIFEELDAPGEWFHNPKTGTLYFYPPAGMDWDGSVEVVRLRHLIEFQGAPPAPV
                     RHLTLRGFIFRHAARTFMETKEPLLRSDWTIYRGGAVLFNGAEDCTVADCEFDQLGGN
                     AVFVNNRNRRVTIRGCDLHDTGATAVAFVGDPQAVRNPLFEYNQRQRFADIDTAPGPK
                     TDNYPADCLVEDCLIRGMGVVEKQATGIQISMSMGITVRHCSIYEASRAGINISEGTF
                     GGHVIEFCDVFDTVRETGDHGSFNSWGRDRYWGLTDVPPGELPKLARLDAVMPTILRN
                     NRWRCDHGWDVDLDDGSSNYEISNNLFLNGGLKLREGFHRRVWNNIAVNNSFHPHAWY
                     EHSGDVVTNNIWMGAYRPAAMSDSLERWGQDIDRNLFTTSEADRLRFAQKGCDEHSLV
                     GDPLFVDPARGDFRVKDGSPALRLGFRNFAMDRFGVMKPALRAIARTPEMPKLATPGP
                     TADTARAANRAKPPWFWLEARIRDLEGEEYSAFGVSRESGGVHLPEVPPASAAARCGL
                     QSGDLVRSVNGRPARTVRDLLRLRDAGEGKGLIVGFVRGQQLKQTTIESQPYAIPESA
                     SAETEFRTVRLANAGVAPVRAVTTRPDTVNEPVATLHDGRLAADYGPVFGNGIRLGLY
                     KADLGTVREISSVATWSFDQNDNRGSQRFTLFGSAAPEDPGWDVADPQKFTPIASVDS
                     RCEGRFHGTRLQGGADCRLGSFRWLVWAVSPVTELGENTAYQEFQVR"
     gene            3645..5108
                     /locus_tag="PKK20_06630"
     CDS             3645..5108
                     /locus_tag="PKK20_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNU99596.1"
                     /translation="MRRSTPIRRRHNPADTEITPQIKHAGDSVGIIFPDHIVSNRSSY
                     SSLLEQGGLGAGTSIVNPQNSNRHSNRHLRIALFAAALLPLGGTSWSAETAALKDVFK
                     DHFMVGTAVNRAITMGAGFRRGPEQAKQDVALVKAQFNQLVAENEMKWALLHPRPGAD
                     GYDFRAADAFVSFGVSNAMDLAGHTLVWHSQTPDWVFAGTNPPPPGVTNAAPTPFANP
                     SASGTNQFGRGRFGRGFGGEPFGRYTGPRASRDEMLERMRDHIHTVVGRYKGRIKVWD
                     VVNEALADGGTNILRNSLWLEIIGPDFIAKAFHYAHAADPEAILRYNDYGLENPAKRR
                     KLITLIKSLQEQKVPVHAIGSQAHVNVSTTFETMDQALADMATLGLPIHITELDVTSA
                     QGGQRGFGADIADNAATAQGGLVSDADRRLAEAYAGLFRAFLKHDKHVEMVTFWGPND
                     ANSWRAGGKPLLFDANSQPKPAFHAVIAEAKAARAKK"
     gene            complement(5224..6357)
                     /locus_tag="PKK20_06635"
     CDS             complement(5224..6357)
                     /locus_tag="PKK20_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU99597.1"
                     /translation="MSDSVKLRETSGLKDCDNDQFFVDSRGRAAQSLLERHMNRIPRI
                     SIVLPARNAAGTLPAALGSIRAQTFVAWELVAVDDGSTDETHRILSSAARADARIRVL
                     SLRPVGIVAALRAGCEAARGEFIARMDADDLMLPERLQRQADFLARHPRIGVVSCRVR
                     YGGDRASQAGYAAHVDWTNALLTPEDMALRRFVESPVAHPSVVFRRELLAQHGGYAEG
                     DFPEDYELWLRWMDAGVRFGKVNAELLVWNDLPARLSRTDARYRTEAFYRTKSLYLAR
                     WLKRHVAADRAFWLWGAGRVTRRRFLALEADGIRFVGFIDIDANKLGRARDGRWVVRP
                     NALPDPARAFILVGVAKRGARELITAELRRLGRQEGRDFLLVA"
     gene            6539..>8033
                     /locus_tag="PKK20_06640"
     CDS             6539..>8033
                     /locus_tag="PKK20_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99598.1"
                     /translation="MKQARANPNGVRRHFHPDRMVAQWLAGVFLFLCPAIALCQPSIG
                     VAQISADSIGLRLTVGQMPSLYSVQFAPSAEALMSHPAVLTSGVAQSVSTNVIALPIS
                     TDVPMMFFRVVEAPLTPNQLTNLTDTGSVQTVSMEGVSMTVPAGTLTAPATAAVVGRT
                     AAEAGIPPVSHGLETFQVFDIQIGNETRFNQDLTLELPYDPARLNPDLPVEDALVVSY
                     WLPESKSWKSVPLTVDPNAGVVRFSTPHLSTWAVRYIARGWQVLKSDNLHARVVFDPT
                     QPVFRGKSQKVPVQDYAQLIADAGEAAYSAYKTAGFIVPEEQWYIILNAAEEGYAFWS
                     GWTGDVYLPSVFDDDEWVRYTVGHELFHSVQNAYYNIYSASGRQWWHEACATYASWHY
                     VLNRDYPLRTPDIKTSDYPALPVSTANGSHEYVTAELLAFIFARSGLTFKAHFDDMEG
                     YGISSTLKRLEQTVSRLAGSDLHRFYRLFCGDYWQRTGGRTGASRYRP"
BASE COUNT         1497 a         2582 c         2570 g         1384 t
ORIGIN      
        1 ataccgtcct ccggcacgtt gaatccgcaa tggacgagcc atgcgggaag gatgggcatt
       61 ccacggcaaa tgtcaattat gaagatgtgc ccccaagttc tatggctcgc gcaggcggtt
      121 catgggcagg ccaagtgcgc aactgcgcaa caacacaact cgatcggact tgacgcagtc
      181 acggcagaat gcataaaggc cctatggtcc cgacgagcgt cggccatcca cacgccatgc
      241 atcgcttgct gcgggaggac tcgcgcagga attgcgtgca tccgggcgtg catgcgcgct
      301 tgaggaggag aactcggatc gcctgcgcca gaaggggagc gtgcaggcct acaagaatgc
      361 gggctgcggc ggacccacgt tgtaccgcca cgccgaaggc gcgggtctgg cattctacga
      421 cggtcgcgcc cagcgcctgc gcaaagaaaa ggtctggatc gccgcggaca aaaacgcgaa
      481 ccccaagcgg cccgggatgt gggtcgcaga cctcgagacc tggaggcgca accattagaa
      541 tccttgaccg gcgaaacatg gagtcaatca gcgcggcgat ccgcgggtcg ccgatcgccg
      601 gcgaggagca ctccgatcgg tgtgtccgga ccgcgatcgg cgcgaaagcg tacccgaagc
      661 cgtcggaaga catcacgttg atcgaggact gatcgagcca tgaagctgtg catcttctcg
      721 gggttgatcc tgaccgcgct gtgcgcggct gccgccgaat tccacgtcag tcccggcggc
      781 gatgatgcca atgccggcac gagggacaag ccgttcgcca cgccggcccg ggcgcagcag
      841 gcggcgcgcg cggctgccgg caaggaaccg gtgacggtct ggctgcgagg cgggacctac
      901 gccctcccgt ccccattggt cttcacgccg gcggattcgg gcactccgga tgcgccggtt
      961 gtgtggcagg cctttcccgg cgaatcgccg gttataagcg gcgggctgaa gctggacctc
     1021 gagtgggaac cgcaccgcaa tggcatcctg aaggccaggg tgcccgcggg attgacttcc
     1081 gatcagctct tcgtcaacgg ccggcgccag ctcatggcgc ggtacccgaa ttatgatgcg
     1141 agcgttcggc cctacggcgg ctacgcggca gatgcgttcg gcgcggagcg ggccgcccgg
     1201 tgggctgatc cggccggcgg ctacatccat gcgatgcact ccgcgcactg gggcggctac
     1261 cactaccgca tcaccggcag gaacgagaag ggcgaaatca tctacgaagg cggctggcag
     1321 aacaatcgcc agatgggcat gcacgcgcag catcggtacg tcgaaaacat cttcgaggaa
     1381 ctcgatgcgc cgggcgagtg gtttcacaac cccaagaccg gcacgctgta tttctatccg
     1441 ccggctggca tggattggga tggctccgtc gaggtcgtgc gcctgcgtca cctcatcgag
     1501 ttccaaggcg cgccgccggc ccccgtcagg cacctcaccc tgcgcggctt catcttccgc
     1561 catgcggcgc gcacattcat ggagaccaaa gaaccgctcc tgcgcagcga ttggaccatt
     1621 taccgcggcg gggcggtgct cttcaatggg gcggaggatt gcaccgtggc ggattgcgag
     1681 tttgaccagc tcggcggcaa tgccgtcttc gtcaacaacc ggaaccgccg cgtgacgatc
     1741 cgcggctgcg acctccatga caccggcgcg actgcggtgg ccttcgtcgg cgacccccag
     1801 gcggtgcgca atccgctctt cgagtacaac cagcgccagc gtttcgccga catcgacacg
     1861 gcgcccggcc cgaagacgga caattatccc gcggattgtc tggtcgagga ttgcctcatt
     1921 cgcgggatgg gcgtagtcga gaagcaggcg acagggatac agatttccat gtcgatgggc
     1981 atcacggtcc ggcactgctc gatctacgag gcctcgcgcg ccggaatcaa catcagcgaa
     2041 ggcaccttcg gaggacacgt catcgagttc tgcgacgtgt tcgacacggt gcgcgaaacc
     2101 ggcgatcacg gcagcttcaa ctcgtggggc cgcgatcggt actggggctt gacggacgtg
     2161 cctcccggcg aattgcccaa gctcgcccgt ctcgacgcgg tgatgccgac catcctgcgc
     2221 aacaaccggt ggcgctgcga ccacggttgg gatgtggatc tcgatgacgg ttcgtcgaac
     2281 tacgagatct ccaacaacct gttcctgaat ggcggtctca agttgcgcga gggctttcac
     2341 cgccgggtgt ggaacaacat cgccgtcaac aactcgttcc atccgcacgc ctggtatgag
     2401 cacagcggcg acgtggtcac gaacaacatc tggatgggcg cctaccgtcc cgcggcgatg
     2461 agcgacagcc tcgagcgctg ggggcaagac atagaccgca acctgttcac cacctccgag
     2521 gccgaccgtc tgcgattcgc acagaagggt tgcgacgagc attccctggt gggcgatccg
     2581 ctgttcgtgg acccggccag aggcgacttc cgggttaaag acgggtcgcc cgcgctccga
     2641 ctcggattcc ggaattttgc gatggatcga ttcggcgtga tgaaacccgc gctgcgggcg
     2701 atcgcgcgga cgccggagat gccgaagttg gcaacacctg gacccacagc ggacacggcc
     2761 cgcgccgcca atcgtgccaa accgccgtgg ttttggctcg aggcgcgcat tcgggatctc
     2821 gaaggcgagg agtactccgc ctttggggtc agcagggaat cgggcggagt tcatctgccc
     2881 gaggtgccgc cggcatcggc cgcggcacgg tgcggcttgc agagcggtga cttggtccgg
     2941 agcgtcaacg gccggccggc gcggactgtg cgcgatttgc tgcggctccg cgatgcaggg
     3001 gaaggcaagg gattgattgt cggatttgtg cgtgggcagc aactgaaaca gacgaccatc
     3061 gaaagccagc catatgcgat cccggaatcc gcgtctgcag aaactgagtt caggaccgtc
     3121 cgtctggcaa acgccggcgt ggctccggtt cgggcggtga ccacccgtcc tgatacggtg
     3181 aacgagccgg tcgcgacgct acacgacggg cggttggcgg ccgactacgg tccggtgttc
     3241 ggcaacggca tccgactcgg tttgtacaag gcggatctgg ggactgtgcg tgagatctcg
     3301 agtgtggcga cgtggtcgtt tgatcagaac gacaaccgcg ggtctcagcg gttcacgctc
     3361 tttggcagtg cggcacccga agatccgggc tgggacgtgg ccgaccccca aaagttcacg
     3421 cccatcgcca gcgtggacag ccgatgcgaa ggccggttcc atgggacacg cctgcagggt
     3481 ggcgcagact gtcggctcgg ctcattccgc tggctggtct gggcggtctc accggtcact
     3541 gagttgggtg aaaatacggc ctatcaggag ttccaggtca ggtagcgggt cttaccctgg
     3601 ccccatgacg acgattccac tcgaagtgcc ggacgaatga agccatgagg cggtcaaccc
     3661 caatccgacg gcgccataat ccggctgaca ccgaaatcac gccccagatc aagcacgccg
     3721 gggacagcgt cggcatcatc tttcccgacc acatcgtttc caaccgcagc agctattcaa
     3781 gcctcctcga acagggtggg ctcggagccg gcacatctat cgtgaacccc caaaactcga
     3841 atcgacactc gaatcgccac ttgcgaattg ccctcttcgc ggcagccctc ctgcccctcg
     3901 gcggcacatc ctggtccgcc gagacggccg cgctcaagga tgtcttcaaa gaccacttca
     3961 tggtggggac cgccgtcaat cgcgccatca cgatgggcgc cgggttccgc cgaggccctg
     4021 agcaggcaaa gcaggacgtc gctctggtga aggcgcagtt caaccaactc gtcgcggaga
     4081 acgaaatgaa atgggcgctc cttcacccgc ggccgggagc ggacggctat gacttcaggg
     4141 ctgccgacgc ctttgtgagc ttcggcgtga gcaacgcgat ggacctcgcc gggcatacgc
     4201 tcgtctggca ttcccagacg cccgattggg tgtttgccgg cacgaatccg ccgccgccag
     4261 gagtcaccaa cgccgccccc accccatttg ccaacccgag tgcgtccgga acaaaccagt
     4321 tcgggcgtgg gcgattcggt cgtggctttg gcggcgaacc cttcggccgc tacacggggc
     4381 cgcgcgcatc gcgcgatgaa atgctcgagc ggatgcgcga tcacatccac accgtcgtgg
     4441 gccggtacaa ggggaggatc aaagtctggg acgtggtcaa tgaggcgctc gccgacggcg
     4501 gcaccaacat cctgcgcaac tcgctctggc tcgagatcat aggtccagac ttcatcgcca
     4561 aggccttcca ctacgcccat gcggcggacc cggaggcgat tctacgctac aacgactacg
     4621 gtttggagaa tccggccaaa cgccggaagc tcatcacgct gatcaagtcg ttgcaggaac
     4681 agaaggtgcc ggttcatgcc attggttcgc aggctcatgt caatgtctcg acgaccttcg
     4741 agaccatgga tcaggccctg gcggatatgg cgacgctcgg cttgcccatt cacatcacgg
     4801 aactcgacgt caccagcgcg cagggtggcc aacgcggatt cggggcggac attgcggaca
     4861 acgccgcgac cgcccagggc ggcctggtca gcgacgccga ccgcagactg gccgaggcgt
     4921 acgcgggcct cttccgggcc ttcctcaaac acgacaagca cgtcgagatg gtcacgttct
     4981 ggggcccgaa cgacgcgaac tcctggcgcg cgggcggcaa accgctgctt ttcgacgcca
     5041 actctcagcc caagcccgcc ttccacgccg tcatcgcgga agcgaaggca gcacgcgcga
     5101 agaagtagga gtgcggcgga cctgcacgga gagatgctac gactcgcacg cacgcgtgtc
     5161 tctcgaccac ttttccgttc tgccacccaa ccaccggcag gccaagggcc cgatgctgag
     5221 ttttcacgcc acgagcaaga aatcccgccc ctcctggcgg cccaggcgac gcaactcggc
     5281 ggtgatcagt tcgcgcgcgc cgcgcttggc cacgccgacg agaatgaacg cgcgcgcggg
     5341 gtcaggcaac gcattcggcc ggaccaccca ccgcccatcg cgcgcgcgtc cgagcttgtt
     5401 cgcgtcgatg tcgatgaagc ccacgaaacg aatcccgtcc gcttccaggg cgagaaagcg
     5461 tcggcgcgtt acgcggcccg cgccccagag ccagaacgcc cggtccgccg ccacgtgacg
     5521 tttgagccag cgggccagat acaggctttt tgtccggtaa aacgcctcgg tgcggtagcg
     5581 cgcgtcggtg cgggacaggc gcgcgggcag atcgttccac acgagcaatt cggcgttcac
     5641 tttgccgaag cggaccccgg cgtccatcca gcgcagccac agttcgtagt cctccgggaa
     5701 atcgccctcc gcgtaaccgc cgtgttgggc cagtagttcg cgccggaaca cgacgctcgg
     5761 atgcgccaca ggcgactcca caaaacgccg caatgccatg tcctccggcg tgagcagtgc
     5821 gttggtccag tcgacgtgcg ccgcataacc cgcctgcgac gcccggtcgc ctccgtaccg
     5881 gacgcggcag gacaccaccc cgatgcgcgg atgacgtgcc aggaaatcgg cctgccgttg
     5941 cagacgctcg ggcagcatca gatcgtccgc atccatgcgc gcgatgaact cgccgcgcgc
     6001 cgcctcgcag cccgcgcgca gcgcggccac gattccaacg ggccgcaggg acaacacgcg
     6061 gatacgggca tccgcccgtg cggcactgct aagaatgcga tgtgtttcgt ccgtcgaacc
     6121 atcgtccacg gccaccagtt cccaggcgac gaacgtctgg gcccggatgc tcccgagcgc
     6181 ggcgggcagc gtgccggccg cgttgcgcgc gggcagcacg atcgagattc tcgggatgcg
     6241 gttcatgtgg cgttcgagca gcgattgagc cgctcgtccg cgcgagtcaa caaagaactg
     6301 gtcgttgtcg caatccttta gcccggatgt ttcacgaagc ttcacggagt cggacatcgg
     6361 ggttccggca aaaccgcgtt gagcggcgtc gtcgggaaac ggcgacggag ggacgccttg
     6421 cgtcctgcgc gtgtgccatc ccgcgttttc ggtgtcgcgt tttcgcgttt tcgggtgttg
     6481 cgtttttcgg gttgattcac gccgggaggg gtccggtaca aaacggcgac ctcctcatat
     6541 gaaacaagca cgcgcgaatc cgaatggcgt caggcggcac tttcacccgg accgaatggt
     6601 ggctcagtgg ttggccgggg tgttcctgtt cctttgtcca gcgattgcgc tttgccagcc
     6661 gagcattggc gtcgcccaga tcagcgcgga ctccatcggg ctgcggctca cggtcgggca
     6721 gatgccgagc ctctattcgg tgcaattcgc gccgagtgcg gaagccctga tgtcccatcc
     6781 ggcggtgctg acctcgggtg tggctcaaag cgtgtcgacg aatgtcatcg cactgccgat
     6841 ctcgaccgac gtgccgatga tgttcttccg cgttgtcgag gcgccgctga ctcccaatca
     6901 gttgacgaac ctgaccgaca ccggcagcgt gcagaccgtg tccatggagg gtgtctcgat
     6961 gaccgttccg gccggcacgc tgactgcgcc cgccacggcg gcggtggtcg ggcggacggc
     7021 ggcggaagcg ggcattcccc ccgtgtctca cggtctggag acgttccagg tgttcgacat
     7081 ccagatcggc aacgaaacgc ggttcaacca ggacctcacc ctggaacttc cgtacgaccc
     7141 ggcgcgactc aacccggacc tgccggtaga agatgccctg gtagtgtcat actggctgcc
     7201 cgaatccaag agttggaagt ccgtgcctct cacggtggac ccgaacgccg gcgtggtgcg
     7261 gttctcgacg ccgcacttgt ccacctgggc ggtgcgctac atcgctcggg gctggcaggt
     7321 gctcaaatcg gacaacctcc atgcccgggt cgtgttcgac cccacccagc cggtttttcg
     7381 gggcaagtcc cagaaggttc ccgtccagga ctacgcgcaa ttgattgcgg acgcaggtga
     7441 ggcggcttat tccgcctaca agaccgccgg cttcatagtg cccgaggagc aatggtacat
     7501 cattctgaat gcggctgagg aaggttatgc tttctggtct ggctggacgg gcgacgtgta
     7561 cctgccctcg gtcttcgatg atgacgagtg ggtgcgttac accgtcgggc acgaattgtt
     7621 tcactcggtg caaaacgcct attacaacat ttactcggcc tccggccgcc agtggtggca
     7681 tgaggcgtgc gccacgtacg cttcctggca ttacgtgctc aatcgagact acccgttacg
     7741 gacgccggat atcaaaacgt cggattaccc ggcgctgccg gtgtcgactg cgaacgggtc
     7801 gcacgaatac gtcaccgccg aactgctggc cttcatcttc gcgcgctccg ggttgacgtt
     7861 taaagcgcac ttcgacgaca tggaaggcta tgggatttcc tcgaccctga aacggctcga
     7921 acaaaccgta agccgcttgg ccggtagcga cttgcaccgg ttctaccgcc tgttctgtgg
     7981 cgattactgg caaagaacag gcggtagaac cggtgcaagt cgctaccggc caa
//
LOCUS       DAOQRO010000136         7998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8621_length_7998_cov_4.351756, whole genome shotgun sequence.
ACCESSION   DAOQRO010000136 DAOQRO010000000
VERSION     DAOQRO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8621_length_7998_cov_4.351756"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="PKK20_06645"
     CDS             <1..1961
                     /locus_tag="PKK20_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99599.1"
                     /translation="LTLVTNANNFTFKSDTTYHIAGIFNLTGTTTIEGGCVIKFAPNS
                     SASLVLHGPVNCQTAPYRPAILTARDDDSVGEIPSASTGNPRGCYYAKPALKIMLGGQ
                     TLHDLRIAHAQTGIYFHDYSVGTNSVRHVQFVRCGTALQVNGYGTAFQEITLHNLLIH
                     DAPQTLAGYSFHARAEHLTVCQSTQLVTDTWGSSYGTRSSIELVNSLLVAIPSLGNVD
                     VSQIRCRSAAQSNTVFQTVGAGGFYLAQPSFRNLGATNIDATLARELRQKTTTPPSIC
                     TASNGADLVLAPRVPRDSDTPDLGYHYDPIDYAISELVIAGTRTLTVLPDACLAAYGP
                     YGMRLEAGACLSAEGTPLRPIRLFRYSLVQEQPSVWGLWTAAPVFVLGTDQSPATTPT
                     APSACFRFVEFLDLANRACHVLSDTSTSRFAEITLRDCLVRGGRLDVTGDATALVRFH
                     NNLFRRAEVLLQAGIQLTAFNNLFRGGRLQLDRILPAGDWVMRDTAFHEVDLLDINGG
                     LIHSHNAYIGPKQERLIRIGSTDKVLSTFPYASGPLGDCYHAATSLINAGSRPAKDAG
                     LWHHTTQANQTKEAETTVDIGFHYLALSSGTDAGLPQDTDADGVADYLEDTNGNGRVD
                     SGETNWRDARDIGLGVRFTRPQRSSPVP"
     gene            2001..7703
                     /locus_tag="PKK20_06650"
     CDS             2001..7703
                     /locus_tag="PKK20_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNU99600.1"
                     /translation="MRLSAQLHRVALASTLALLHEAAFTSTAPIPTPPPALSGRLTDT
                     APPPGQSPTARSCGLAALSALANRVGATIEASFPTPAPDLESPSTNLADLERAAAQIG
                     LDMKPARRTGTDPWPAASIVHWRIGHFSAVIDRAGSRVLLDDPMLGGKVWRDARWLDC
                     HASGYGLVPASRLDSNWTSLSPEEAACIRGGQRIYPPTLYDDDDEECPTDEEIASQPM
                     DPEAPNPGELTACPQPETCPPGHGEEECDGLGMPVWRVSEPLINLWIKDVPLYYTMSN
                     GRIMPLVLRYKQRGSPLSTNVFGFGNSWECSWLSYFDTAADSSVKFRVPGGGKRRYDG
                     VLGGPVRGAGCEQVAATVRGDGSVSFTIIYHSFCANQYAQEFPGAETNTLHLLSEKMD
                     PHGRVIRFVYSTNATMILLSRVIDYDGKVSQLFYTNRQFPRFVTSVRDPYGREARFQY
                     DTNGNLVRIVDMAGITNAFRYSSDGFLNAMITPYGMTRFRTWSVETGLEDLPSSRSIG
                     ITLPNGERQAYAYYERLGTECVPDYYSDFPQLDSEVPEPVPVGPLNLRGSFFWNSRQL
                     ALVTNDWPTFSPQDFRLARWRHWDMKIVGNGPRVGTRLLLERAPSPDGITDGHRRWYT
                     YFYDYWSGNDTAWPSQQAERLPDGTVRYVSWQRNEWGLPVSIESTFSDGSARGVRQTW
                     LWYDNSIGCRLTEVYGPESNTSQWPALTRSYAYNLNGQITNMVNALGETTSATYHPVN
                     RHLSSVTYPFGLTITNKFDAIGFLTQRVWIAASGAPLATNACQWSQGRVSTTVDARGL
                     KTTCFWDALGRLTGRAYPDGTTISNWYVKLDGVPFTNGTGGLTLLDRTRFKDRLGNLT
                     DFACDALRQPIAITNALGQATRFTWCNCGTVESMTDPMGHTTSLEHDLNGRPVAVHLP
                     GGSMLRIALDALGRPTNVTDTVHSLDLTYNNQGLQTTLATATSVVQRVDYDGLDRPRL
                     VDLPTGAQYEFAYDPLGRLVRRTECRSQGSEWIGYAPGVPWPATYTNQTGTNVLLLAY
                     DALGRLTRQIQGTVGTGSFLGAATNSFTYSAAGDLLSILDALNRRTTWTYDSYGRTVK
                     KVDAAGRVVWTNAYDANHQLVAHWTPARSNLTSYTYDTLGRLTRVDFSAPTTPDLAFA
                     YDAAGRLTAVTDGIGTTRFAWHPSGLLAGEDGPWANDNVFFGRDSTLALRTLALEQPD
                     APYWLQTFAYDGDGRLARLVSPAGDFTYAYRSNQMLPAAIARLDGAAIAQDHDALGRL
                     LRTTLSNPQGEVLNRHLYHYNAAHQRTRQTFAEGNTIDYAYDALGQVISARGYEPNRT
                     TRQHEQFTYRYDAAGNLVSRQNGASTLTLSINLLNQLATATRSTATLVTGMTTPAATN
                     VTVNGQTAALYQDRTFARPNLALPSGPATLTAVARDALGRSATDTITVTLPGSAAFAY
                     DANGNLIRDGRRLFTYDDRDQLVAVIVTNGVNESTLSEFLYDAFGRRRILREKTWQTN
                     QWVQTDETRYVYAGLLVLQERGADNQPRVTYTRGPDLSGDLDQAGGIGGLLAMTLHTP
                     GDARHYYYHADGSGNVTALIDSRHNVVARYRYDPFGNLQGLAGPLAEVNRIRFSSKEF
                     HPPSGLSYFGFRFYDPNLQRWINQDPMAEMVGVNLTRFADNAPLKYIDANGESPTLFL
                     GGIGMIVGGVIGGISSHLRGDGFWQGAYVGAISGGLIGLTGGLAAAGTMAAAEALGAG
                     AAIGAVAGGAVGSMVGDLVAQNIEALSGDRDCFNWNRTALSGVIGGSLSSAACRAFYS
                     TQPNAALWSGERGRQLAERWAAANAGQTIGSTPGARVVEALTPPHLPSFLWKNLYRPI
                     WAKGSQIFVESFSEHARFQIFLPIPWRNSSILCTEEIASLIRMNPINTPVVVPYVTAP
                     "
BASE COUNT         1529 a         2982 c         2088 g         1399 t
ORIGIN      
        1 ccctgacgct ggtcacgaac gcgaacaact tcactttcaa gagcgacacg acctatcaca
       61 tcgccggcat cttcaatctg accggcacga ctaccatcga gggtggctgc gtcatcaaat
      121 tcgccccgaa ctcatccgca agcctcgtcc tgcacggacc ggtgaattgc cagaccgctc
      181 cctatcgtcc ggccattctg acggcgcggg acgacgattc ggtcggcgag atcccgtctg
      241 catcgacggg caaccccaga ggctgctact acgcaaaacc ggcgctcaag atcatgctcg
      301 gcggccagac tctccatgac ctgaggatcg cccacgccca gacaggaatc tacttccacg
      361 actacagtgt cggcaccaac agtgtgcgcc acgtgcagtt tgtccgatgc ggcaccgccc
      421 tccaggtcaa tggatacggc accgcttttc aggagatcac cctccacaac ctgctcatcc
      481 acgacgcgcc tcagacgctt gcgggctaca gctttcatgc cagggccgaa catctcaccg
      541 tctgtcaatc gacccaactg gtcacggata cctggggatc ctcctacgga acccggagtt
      601 cgatcgagct tgtgaacagt ctgctcgtcg caatccccag cctgggcaac gtggacgtga
      661 gccagatccg ttgccggtcg gctgctcaaa gcaacaccgt cttccaaacc gtcggcgccg
      721 gcggcttcta cctggcgcaa ccctcgttcc gcaacctcgg cgccacgaac atcgacgcca
      781 cactcgcccg cgaactcaga cagaagacca ccaccccgcc gtcaatctgc accgcctcga
      841 acggcgccga cctcgtgctc gccccgcgcg ttccgcgcga ctccgacacc ccagatctcg
      901 gctatcacta tgacccgatc gactatgcga tcagcgaact tgtcattgcc ggaacgcgca
      961 ccttgaccgt cctgcccgac gcctgcctgg ctgcctatgg cccctatggc atgcgtctcg
     1021 aagccggagc ctgcctgagc gccgagggga cacccctgcg cccgatccgc ttgttccgtt
     1081 actccctggt ccaggaacag ccgtccgtct ggggactctg gaccgccgcc ccggtcttcg
     1141 tcctgggcac ggatcaaagc cccgccacca cgcccacggc cccatccgcg tgttttcgct
     1201 tcgtcgagtt cctcgatctg gccaatcgcg cctgccacgt cctaagcgac accagcacca
     1261 gccgtttcgc cgagatcacg ctcagggact gcctggtgcg gggcggccga ctcgacgtga
     1321 cgggcgacgc caccgccctc gttcgcttcc acaacaacct cttccggcgc gccgaggtcc
     1381 ttttgcaggc gggaatccag ttgaccgcat tcaacaacct gttccgcggc ggccgcctcc
     1441 aactcgaccg catcctgccc gcgggcgatt gggtcatgcg cgacaccgca ttccacgaag
     1501 tggatttgct ggacatcaat ggcggattga tccacagcca caacgcctac atcggcccca
     1561 agcaggagcg gctcatccgc atcggctcca ccgacaaggt cctgtccacg ttcccttatg
     1621 ccagcgggcc gctgggcgat tgctaccatg ccgcgaccag tctgataaac gccggcagtc
     1681 ggccagccaa ggacgccggc ctctggcatc acacgaccca ggccaaccaa accaaggagg
     1741 ccgagacgac cgtcgatatc ggattccact acctcgccct cagttcgggc accgacgccg
     1801 gactccccca ggacaccgat gctgacggcg tcgcggatta cctcgaagac acgaacggaa
     1861 atggccgggt cgactcgggc gaaacgaatt ggcgtgacgc acgcgatatc ggactcggag
     1921 tgcgcttcac gcgcccgcaa cgctcatccc ccgtccccta atcccccgca attcatgcct
     1981 ctcaaaccaa ggagtacccc atgcgtctca gcgcccagct tcatcgagta gccctcgcca
     2041 gcacgctcgc cctgctgcac gaggccgcgt tcacctcgac cgccccgatc ccgaccccgc
     2101 ctcctgccct gtcgggccgc ctgacggaca ccgccccgcc gccgggtcag agtccgactg
     2161 ctcgatcctg cggccttgct gccctgtccg ccctggcgaa tcgagtcggg gcgacaatcg
     2221 aggcgagttt tccaacaccc gcgcctgatc tcgaatcgcc ctccaccaac ctcgcggatc
     2281 tcgaacgggc cgctgcacaa atcggcctcg atatgaaacc cgcgcgccgg accgggacgg
     2341 atccctggcc cgcggcgtcc atcgtgcact ggcgcattgg ccatttctcc gcggtgatcg
     2401 accgcgccgg cagccgcgtg ctcctggacg atccgatgct cgggggcaaa gtctggcgcg
     2461 atgcccgctg gttagactgc cacgccagcg gttacgggct tgttcccgca agccgactcg
     2521 attccaactg gacgtcgctc agccccgaag aggccgcctg catccggggc ggtcagcgca
     2581 tctacccgcc cacgctctac gacgacgatg acgaggaatg ccccaccgac gaggaaatcg
     2641 cgtcccagcc catggacccc gaggcgccta atccgggcga actgacagcc tgcccgcagc
     2701 ccgagacctg cccgcccggg catggggagg aggaatgcga cgggttgggc atgcctgtgt
     2761 ggcgcgtctc cgaacccctg atcaatctct ggatcaagga cgttcccctc tactacacga
     2821 tgtccaacgg gcgcatcatg cccctggtgc tccgctacaa acagcgtggc tcgcccctca
     2881 gcaccaatgt cttcggtttc ggcaattcct gggagtgttc ctggctcagc tactttgaca
     2941 cagccgcgga ctccagcgtc aagttccggg tccccggcgg aggcaaacgc cgctacgacg
     3001 gcgtcttggg cgggccggtg cgaggggccg gttgcgaaca ggttgccgcc actgtccgcg
     3061 gcgacggttc ggtcagcttc accataatct accattcgtt ctgcgccaac cagtacgccc
     3121 aggagttccc gggcgcggag accaacaccc tccacctcct ctccgagaag atggaccccc
     3181 acggacgcgt catccggttc gtctacagca ccaatgccac gatgattctg ttgagccgtg
     3241 tcatcgacta cgacggcaaa gtctcgcagt tgttctacac caaccgccag tttcctcggt
     3301 tcgtcacctc ggtccgagat ccctacggac gcgaagcccg attccaatac gacaccaacg
     3361 gcaacctggt ccgcatcgtc gatatggccg ggatcacgaa cgccttccgc tacagttcgg
     3421 acggctttct caacgccatg atcaccccct atggcatgac acgctttcgc acctggtcag
     3481 tcgagacggg cctcgaggac ttgccctcgt cgcgttctat cggaatcaca ctccccaatg
     3541 gcgagcgcca ggcctacgcc tattacgagc gcctcggcac cgagtgcgtt cccgactact
     3601 actcggattt tccccagctc gacagtgagg tgcccgaacc ggtcccagtc ggcccgctca
     3661 acctgcgcgg cagcttcttc tggaacagcc gccaactggc tctggtgacc aatgactggc
     3721 ccacattcag tccgcaagac ttccgcctgg cccgctggcg ccactgggac atgaagatcg
     3781 tcggaaacgg accccgagtc ggcacacgcc tgctgctcga acgggcgccc agccccgacg
     3841 gcatcaccga cggccaccga cgctggtaca cctatttcta tgactattgg agtggaaacg
     3901 acacggcctg gccctcccag caggccgaac gcctgcccga tggcaccgtc cgctacgtca
     3961 gttggcagcg caatgagtgg ggcctgccag tctcgatcga gagcacattc tcggacggtt
     4021 ccgctcgggg cgtgcgtcag acctggctct ggtatgacaa cagcatcgga tgccgcctca
     4081 ccgaggtcta tggacccgag tccaacacct cgcaatggcc cgcgcttacc cggtcctacg
     4141 cctacaatct caacggccaa atcaccaaca tggtcaatgc cctcggcgaa accacctccg
     4201 ccacctatca ccccgtcaac cgtcacctct ccagcgtcac ttacccgttc ggactcacca
     4261 tcaccaacaa gttcgatgcg atcggcttcc tcacccagcg cgtctggatc gccgccagcg
     4321 gcgcgccgct cgccaccaac gcctgccaat ggagccaggg ccgcgtcagc acaacggtcg
     4381 atgcgcgcgg actcaagacg acctgcttct gggatgccct gggacgcctg accggacgcg
     4441 cctacccgga cggcaccacc atttccaact ggtacgtcaa gctggacggc gtcccgttca
     4501 ccaatggcac cggcggcctg accctgctgg accgcacccg gttcaaggat cgcctcggca
     4561 acctcaccga cttcgcctgc gacgcgctgc gccagccgat cgccatcacc aatgccctcg
     4621 ggcaggccac ccgcttcacc tggtgcaact gcggcacggt cgaatcgatg accgacccca
     4681 tgggccacac gaccagtctc gaacatgacc tcaacggacg gcccgtcgcc gtccacctcc
     4741 ccggcggctc gatgctccgc attgccctcg atgctctcgg acgcccgacg aatgtgaccg
     4801 acaccgtcca ttccctcgat ctcacttaca ataaccaggg cctgcaaacc acgctcgcca
     4861 cggcaaccag cgtggtccag cgcgtggact acgacggctt ggatcgccca cgcctggtcg
     4921 acctgcccac aggcgcccag tacgagttcg cctacgaccc gctcggacgc ctcgtccgac
     4981 gcactgaatg ccgcagccag ggatctgaat ggatcggtta tgcccccggc gtcccctggc
     5041 ccgccaccta caccaaccaa accggcacca atgtcctcct gctggcttac gacgccctgg
     5101 gacgcctcac ccgccagatc cagggcaccg tcggcaccgg ctccttcctc ggcgccgcga
     5161 ccaacagttt cacctacagc gccgccggag atctcctatc catcctcgac gctctcaacc
     5221 gccgcacaac ttggacctac gactcctacg ggcgcaccgt gaaaaaagtc gacgccgccg
     5281 gacgcgtcgt ctggaccaac gcctacgacg ccaatcacca actggtcgcc cactggaccc
     5341 ccgcccgctc caatctcacc agctacacct acgacaccct gggacgcctc acccgagtcg
     5401 atttcagcgc cccaactacc cccgacctcg ctttcgccta tgacgcggcc ggacgcctca
     5461 cagccgtcac cgacggcatc ggcaccacgc gattcgcctg gcatccgtcc ggcctcctgg
     5521 ccggcgaaga cggcccttgg gccaatgaca acgtgttctt cggacgcgat tccaccctgg
     5581 ccctgcgcac gctcgccctc gaacaacccg atgcgcctta ctggctgcag accttcgcct
     5641 atgacggcga cggccgcctc gcgcgcctcg tgtcccccgc gggcgatttc acctatgcct
     5701 atcggagcaa ccaaatgctg cccgccgcca tcgcccgtct cgacggcgcc gccatcgccc
     5761 aggaccacga cgccctgggc cgcctcctgc gcaccaccct gtccaatccc caaggcgaag
     5821 tgctcaaccg ccacctctac cactacaatg ccgcccacca acgcacccga caaaccttcg
     5881 cggaaggcaa caccatcgac tacgcatacg acgcgctcgg ccaggtgatc tccgcccgcg
     5941 gttacgaacc caaccgcacg acgcgccagc acgagcagtt cacttaccgc tacgacgccg
     6001 ccggcaacct cgtctcccgc caaaacggcg cctcgacgct caccctctcc atcaacctcc
     6061 tcaaccaact cgccaccgct acccgcagca cggccacgct cgtcacgggc atgaccactc
     6121 ccgccgccac caacgtcacc gtcaacggcc agaccgccgc tctctatcag gaccgcacct
     6181 tcgcccgacc gaacctcgca ttgcccagcg gccccgccac cctgaccgcc gtggcacggg
     6241 acgccctcgg acgatccgca accgatacca tcaccgtgac tctgcccggc tccgccgcct
     6301 tcgcctacga cgccaacggc aacctcatcc gcgacggccg ccgcctcttc acctacgacg
     6361 accgcgatca actcgtcgcc gtcatcgtca ccaacggcgt caacgaatcg accctctccg
     6421 agttcctcta cgacgccttc ggacgaagac gcatcctccg tgagaaaacg tggcaaacca
     6481 accagtgggt tcagaccgac gaaacccgat acgtttacgc cggtctgctc gttcttcagg
     6541 agcgcggagc cgacaatcag ccgcgcgtca cttacactcg cggacccgac ctcagtggcg
     6601 acctcgatca ggcaggcggc atcggcggcc tcctcgccat gaccttgcac acccccggcg
     6661 atgcccggca ttactactac cacgctgacg gcagcggcaa cgtcaccgcc ctcatcgact
     6721 cccgacacaa cgtcgtcgcc cgctatcgct acgatccctt cggaaacctc cagggcctcg
     6781 ccggccccct cgctgaggtt aatcgcatcc gattctcatc caaagagttt catccgccct
     6841 ccggcctctc ctacttcggc ttccgcttct acgaccccaa cctccaacgc tggatcaacc
     6901 aggatccgat ggcggaaatg gtaggtgtga acctcactcg ctttgccgat aatgcaccgc
     6961 tcaagtacat tgacgcaaac ggtgaatctc ccacgctgtt tcttggtgga attgggatga
     7021 ttgttggcgg tgtcatcgga ggcataagct ctcaccttag aggagacggg ttttggcaag
     7081 gcgcctacgt aggtgccata tcgggtgggc ttataggtct taccggcggc cttgctgctg
     7141 cgggcacaat ggcggcagct gaggccctgg gtgccggagc tgctatcggc gctgtcgccg
     7201 gaggcgctgt cggatcgatg gtcggagatc tagtagctca gaacattgaa gcgctcagtg
     7261 gggacaggga ttgtttcaat tggaatcgta cggcacttag cggtgtcatt ggtggcagtc
     7321 tgtcaagcgc agcatgccgg gcattctact ccacccaacc aaacgcggct ctatggtccg
     7381 gggagcgtgg gagacaactg gcagagcgtt gggctgctgc gaatgcaggg caaacaattg
     7441 gctcaacacc aggtgcacga gttgtagaag cgctcacacc tccgcatctt ccgtccttct
     7501 tgtggaagaa tctctatcgg cctatttggg ctaaaggctc gcagatcttt gttgagagct
     7561 tttcggagca cgcacgcttt cagatattcc tgcctattcc ctggcgaaat agcagtattc
     7621 tctgcaccga ggaaattgcg tcactcatcc gcatgaatcc cattaacacc cctgttgttg
     7681 ttccctatgt tacagcaccg tgagcgatta ctggggaatc aacgggatac acctcaccgt
     7741 cacctttacc cgtcctccgg gcacgctctc gcatcggatg ggagagggcg aggtgaggaa
     7801 tctgtgaatg gtaatccagc caacttggcg gtaacacatt accccactcg tgatggatgg
     7861 tgtgcacacc tctgcctcgg catgaaggaa gcgtgcagtc ccagtatatc gcagtcaatt
     7921 gtcttgccaa acgcgcctcg atatctagcc tggatttcca cggaccacca ttagcctaag
     7981 tttccgcctt gccggtct
//
LOCUS       DAOQRO010000137         7967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8678_length_7967_cov_6.008215, whole genome shotgun sequence.
ACCESSION   DAOQRO010000137 DAOQRO010000000
VERSION     DAOQRO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8678_length_7967_cov_6.008215"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKK20_06655"
     CDS             complement(<1..742)
                     /locus_tag="PKK20_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99601.1"
                     /translation="MTNPSSMPRRAFLKRAGTASLGAATLVATPSLFLNRTRAQTGSP
                     PSEAIRVGIIAVGGQGRSNMMAPTMAKHVVAVCDVDKSHVAQAQKQLKEKTGIEAKGY
                     GDYRALLENKEIDAVLIATPDHWHALPAIHACQAGKDVYVEKPLTLTIHEGRAMVKAA
                     RYYNRIVQTGSQQRSWRDEKFRRACEYVRSGRIGDIKTVRVGLPGVNWTKEPPVPDSA
                     PPAELDYDMWLGPAPERPYNKHRVHYYFR"
     gene            905..3868
                     /gene="uvrA"
                     /locus_tag="PKK20_06660"
     CDS             905..3868
                     /gene="uvrA"
                     /locus_tag="PKK20_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNU99602.1"
                     /translation="MAKEFIRIGGARVHNLKNLSLEIPRERLVVITGPSGSGKSSLAF
                     DTIFAEGQRKYMESLSAYARQFLDLIEKPDVDFIEGLSPAIAIEQRTAGANPRSLIAT
                     TTEIYDYLRLLYAHVGQPHCPLTGLPIASQTATEIVDRIAALPPRTRVMLLAPVVRKQ
                     RGEFRDVLERLTRQGFVRARLDGEMYELGGNVRARLDPKRKHTIEVVVDRLVIDAAVR
                     TRLADSVETALKWGEGCVLALEQPPGADAAAGWTETLHSSRMYSPATGLSYDTPTAKH
                     FSFNAPFGACAVCHGLGKKPVFDDHLVIPDSRKTLAQGAVAAWRHGGKRMVAYYKSLL
                     KGLAAHHGVSLETPFENLPEAFKRVLLHGSGETPIEFTFWRAGKMSRLSRPFEGVLPN
                     LDRLYRESESEFTRNRLKAFMSQQTCDACGGRRLKPEILAVTLEAADGAGQAGATAPR
                     APEATRSAISGPGTGPSIPGLSIMDVCRLSVEQADRFLRDVKLTEFQQKVAGEIVKEL
                     RARLGFLRNVGLGYLTLDRESGSLSGGEAQRIRLATQIGAGLVGVLYILDEPSIGLHQ
                     RDNDRLLHTLEGLRDLGNSVLVVEHDEETIRRADYLIDLGPGAGVRGGELVACGTVAE
                     VMANPQSLTGRYLKGDLAIPIPRRRGKPVGDKGWLTVVGARENNLKSIDVRFPLGTLT
                     CVTGVSGSGKSTLVDDILRRALARRLTGASERPGLHTGIEGIENLSKMVVIDQTPIGR
                     TPRSNPATYTGVFNQIRDLFARLPAAKVRGYSSGRFSFNVRGGRCEKCRGDGVLKIEM
                     NFLPPVYVPCEACGGRRYNRETLEITYKGLTIADVLDLTVDEAIPFFRALPDIADACV
                     TLAEVGLGYLRLGQSATTLSGGEAQRLKLATELSRRSLGHTLYLLDEPTTGLHFHDIA
                     KLLEVLVRLRDAGNTLIVIEHHLDVIKCADWIIDLGPEGGADGGRVVAAGTPDQISAN
                     PASHTGRYLRRALAR"
     gene            4117..6852
                     /locus_tag="PKK20_06665"
     CDS             4117..6852
                     /locus_tag="PKK20_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99603.1"
                     /translation="MLATGHTFRIILGIALIAGLALGGLRGRAASSDEDRAYEVAIKM
                     FQDGLFEFADRECAKFAAAYPNSERLADVVVVQVQAKIKLNQFDDALALLTERAPAAG
                     PRADEFAFWRAEILVQKGQLGPAADAFAHLTATFPQSPRSLNAAYREAYFRSTLGETD
                     RAVALLRDPTGAFVRTANAHPEDEFAVRGRLLLAEILSSRADLQGAEEALAAIGDRPL
                     PPELGWERGFLMARLKVEMGKIQEAYPLSTNLTASIPASIPLERRAAALLIEADLLDR
                     MGQADSALQAYERILAESPAGPLLEQAIARYIALSLRAPDMATPVQRFEAVLAAHPNN
                     AALAPVRLTLGELKLKQYYASRTAAAPGAPANLALATNLLGQALAQFDWVATNRPAGP
                     LFGRAQLNRGWCHWEEGPQSLAEGLRAFRDAAAHLSSPADQNIARFKWADCQLRLSDI
                     PGAISNYWIVATNAPATAGATNDLATEALAQIVRAGVEAGDLANASTALNHLLRADPT
                     GQLADRPTLLVGQALAQFGQPQAARTLYDEFLRRYTNSARLPEIRLALAETFELERGW
                     EAAHRAYAAWLGAYSNNPAVSTNMIAQATFDLARVAYRAQPDTNALALLTNFVRLYPY
                     HTNAPLAQYLVGEYAFGQGDYAGAELYFLDRSLTQNTNLLWGELTYRARLMAGRAAVA
                     RQSYRSARDHFDWLITNGPLHVANSPVPIPIVAEAYLFRGDTFVLEPTEAETNSLARF
                     GEAINAFSKITERFPTNEFAPLAWGRIGECHFQLATLDPKRYDSAANAFRRVLESPAD
                     ASLRSQAEWKLGVVLEKQAQLKPPPERAALSEQALDHYLRVLYAKNLRPDEQPDPYWL
                     KRAGLSAAELAESLKKWDVALGLTRRLITDLPILRPRLEKKIEDLQAARQSGDTP"
     gene            7214..>7967
                     /locus_tag="PKK20_06670"
     CDS             7214..>7967
                     /locus_tag="PKK20_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNU99604.1"
                     /translation="MNIEKPITTSDTPARLGIVGRRVGRHLAGLTLLAVLTILPDAVQ
                     AQFLYTTDRNRITITRYTGPGGAVTIPETINGLPVVGIADKAFEGSVDMTSVTIPASV
                     TTIGAWVFKGCTSLAAIEVRTSNTAFRSIEGVLFNKMQTKLLACPGAREGAYAVPGSV
                     TSIETGAFHGCARLTSLTIPRNVTAIRAGAFGGCTGLTSVALPERLTKIEAGLFEGCV
                     NLTSVAIPTTVTLVGSGAFRACTGLTAITIPNA"
BASE COUNT         1417 a         2653 c         2535 g         1362 t
ORIGIN      
        1 agcggaaata gtaatggacg cggtgcttgt tgtagggacg ctccggcgcc ggccccagcc
       61 acatgtcgta gtcgagctcg gccggcggcg cgctgtcggg caccggcggc tccttggtcc
      121 aattcacccc cggcagaccg acgcgcacgg ttttgatgtc gccgatacgc cctgaccgga
      181 cgtactcgca ggcgcggcgg aatttctcgt ctcgccagga gcgctgctgg ctgccggttt
      241 ggacgatccg gttgtaatag cgggcggctt tgaccatggc ccggccttcg tggatggtca
      301 gggtcagcgg cttctcgaca tacacatcct tgccggcctg gcaggcgtgg attgcgggga
      361 gggcgtgcca gtgatcgggc gtggcgatga ggaccgcgtc gatctccttg ttctcgagca
      421 aggccctgta gtctccatat cccttggcct cgatgccggt cttttccttg agttgtttct
      481 gggcttgcgc cacgtgactc ttgtcgacgt cacagacggc gaccacgtgt ttggccatgg
      541 tgggcgccat catgttgctg cggccttgcc cgccgacggc gatgatcccg acgcggatgg
      601 cttcgctggg cggcgacccg gtctgtgcgc gggtgcgatt gaggaaaagg ctgggggtgg
      661 cgacgagcgt ggcggcgccg agcgacgcag tcccagcgcg tttgaggaaa gcccgacggg
      721 gcatcgacga aggatttgtc atacggatgc ttatccggtt tttggggcgc gatgtcaacg
      781 ctcccgggcc ggccaacaga atccttgaaa ggcgcaaatg agaggtgcga cagttgcgtc
      841 gatcgcgccg agttctcggc gctcaagatt ccaggcggac gtttggtgag caggatttgc
      901 cagtatggct aaggaattca tccggatcgg cggcgcgcgc gtccacaatc tcaagaacct
      961 ctcgctcgaa attccgcgcg agcggctggt ggtgatcacg gggccgagcg gctcgggcaa
     1021 gtcgtcgctc gccttcgaca ccatcttcgc cgagggacag cgcaagtaca tggagtcgct
     1081 ttccgcgtac gcccgccagt tcctagattt gatcgagaag ccggacgtgg acttcatcga
     1141 gggcctgtcg cccgcgattg ccatcgagca gcggaccgcc ggggccaatc cgcgctcgct
     1201 cattgcgacg acgaccgaga tctacgacta cctccgcctc ctctacgccc acgtcggcca
     1261 gccgcactgc cccctgaccg gcctcccaat cgcctcgcag accgcgaccg agatcgtgga
     1321 cagaatcgcc gcgttgccgc ctcgcacccg agtcatgctc ctggctcccg tcgtccgcaa
     1381 gcagcgcggc gaattccgcg atgtcctcga acgcctgacc cgccaggggt tcgtccgggc
     1441 gcgcctcgat ggcgagatgt atgagctggg cggcaacgtg cgggccaggc tcgatcccaa
     1501 gagaaaacac acaatcgagg tcgtggtcga tcgcttggtg atcgacgccg cggtccggac
     1561 ccgactggcc gattccgtcg agaccgcctt gaaatggggt gaggggtgcg ttctggcgct
     1621 cgagcaaccg ccgggagccg acgcggccgc gggctggacg gagaccttgc attcgagccg
     1681 gatgtacagt cccgccacgg gcctgagcta tgacaccccg acggcgaagc atttctcgtt
     1741 caacgcgccg tttggcgcgt gcgcggtctg tcacgggctg gggaagaagc ccgtctttga
     1801 cgaccatctg gtgattccag attcccggaa aacgctcgcg cagggggcag tggcggcctg
     1861 gcggcacggc ggcaagcgga tggtcgcgta ctacaagagc ctgctcaagg ggctggcagc
     1921 ccatcacggc gtcagcctgg agactccgtt cgagaacctg cccgaggcgt tcaagcgggt
     1981 tctgctccac ggatcgggcg agacccccat cgagttcacg ttctggcgcg ctgggaagat
     2041 gagcaggttg agccggcctt tcgagggggt cctcccgaat ctcgatcgcc tctaccgcga
     2101 gagcgagagc gaattcacgc gcaaccggct caaagccttc atgagccagc aaacgtgcga
     2161 cgcctgcggc gggcggcgcc tcaagcccga gattctcgcc gtgacgttgg aagcggcgga
     2221 cggcgccggc caggcgggtg cgacggcgcc ccgagcgccc gaggcgactc gatcggcgat
     2281 ctccggcccg gggactggcc cgtcgattcc cggtctttcg atcatggatg tgtgtcgact
     2341 ctcggtcgag caggcggatc gattcctgag ggatgtgaag ctgacggaat tccagcagaa
     2401 ggtggccggc gagatcgtca aggagttgcg ggcgcgcctg ggtttcctca gaaacgtcgg
     2461 tctggggtac ctcactctcg accgcgagag cggcagcctc agcggcggag aggcccagcg
     2521 aatccgtttg gccacgcaga tcggcgcggg cctggtcggc gtgctctaca tcctggatga
     2581 gccgagcatc gggctgcacc agcgcgacaa cgaccgtctg ctgcacacac tcgaaggcct
     2641 gcgcgacctg ggcaattccg tcctcgtggt cgagcacgac gaggagacca tccgccgcgc
     2701 cgattacctg atcgatctgg ggcccggggc cggggtgcgg ggcggtgaac tggtcgcttg
     2761 cggtacggtg gccgaggtga tggccaatcc acagtcgttg accggacgtt atctcaaggg
     2821 cgatctggcg atcccgatcc cacgtcggcg cggaaagccg gtgggcgaca aaggctggct
     2881 cacggttgtg ggggcgcggg agaacaatct gaagagcatc gacgtccgat tcccgctcgg
     2941 gacgctcaca tgcgtgacgg gcgtgagcgg gtcgggcaaa agcaccctgg tggacgacat
     3001 cctgcggcgt gccctggcca ggcgtttgac cggtgccagc gagcggccgg gcctgcacac
     3061 cggcatcgaa ggcatcgaga acctgagcaa gatggtcgtg atcgaccaga caccgatcgg
     3121 gcgcaccccg cggagcaacc ccgccaccta cacaggcgtc ttcaaccaga ttcgcgacct
     3181 gttcgcgcgc ctgccggcgg ccaaggtccg cggctactcc tcggggcgat tcagcttcaa
     3241 cgtccggggg gggcgttgcg agaaatgccg tggagacggc gtcctcaaga tcgagatgaa
     3301 cttcctgccg ccggtctatg tcccatgcga agcgtgcggc gggcggcggt acaatcgcga
     3361 gactctcgag atcacgtaca aaggactcac catcgcggac gtgctcgatc tgacggtcga
     3421 tgaagcgatt ccgtttttcc gcgcgctgcc cgacatcgcc gatgcctgtg tcacgctggc
     3481 tgaggtcggt ctgggttacc tgcgcctggg ccagtccgcc accaccctca gcggcggcga
     3541 agcgcaacgg ctcaagttgg cgaccgagtt gagcaggcgg tcgttggggc ataccctcta
     3601 cctgctcgac gaaccgacca ccggcctcca cttccacgac atcgccaagt tgctcgaggt
     3661 cctggtcagg ctgcgcgacg ccggcaatac cctgatcgtc atcgagcatc atctcgacgt
     3721 catcaagtgc gcggactgga tcatcgatct cgggcccgag ggcggggctg acgggggccg
     3781 ggtggtcgcc gccggcacgc cggatcaaat cagcgccaat ccggcgagcc ataccggacg
     3841 ctacctgcgc cgggccctcg cgcgctgagc ggggcggctc gcccgcgctt ttcgtagcgc
     3901 agcaaagccg cagccgaagt ctcttctttc aagaaaggaa gaaatgtaca gagacttgca
     3961 caccccgatg gtagataggg cactcaggct tcacaggatg aactctatgc gcgcttgaaa
     4021 gggcgcagtg catagaatcc ttgaaatgcc gcatcgagcg ccgacaatga gggcgaacct
     4081 gcccccggta agccgggggt ttcaagaaat cgagtcatgc tcgccactgg ccatacgttc
     4141 cggatcattc tgggcattgc gctgatcgca ggcttggcgc tgggagggtt gcgcgggcgc
     4201 gccgcgagtt cggatgagga tcgggcatac gaagtggcga tcaagatgtt ccaggacggc
     4261 ctcttcgaat tcgcggatcg ggaatgcgcc aagttcgcag ccgcctaccc gaattcggaa
     4321 cgactcgcgg atgtcgtcgt tgtccaggtc caggctaaaa tcaaacttaa ccagttcgac
     4381 gacgccctcg ccctcctgac cgagcgcgcc ccggccgccg ggccgcgggc ggatgaattc
     4441 gcgttctggc gcgctgagat tctcgtgcaa aaaggacagc tcggccccgc ggccgacgcc
     4501 ttcgcacatc tgacggcgac attccctcaa tcgccgcgtt ccctgaacgc cgcctaccgc
     4561 gaggcttatt tccgttccac tttgggcgaa accgatcgag cggtggcctt gctgcgggac
     4621 ccgactggcg ccttcgtgcg gaccgccaat gcgcatcccg aggacgaatt cgccgtgcgg
     4681 gggcggctgc tgctcgccga gattctcagc agccgtgccg atcttcaggg ggccgaggaa
     4741 gcgctcgcgg ccatcggcga ccgccccctg ccgcccgaac tcggctggga gcgcgggttt
     4801 ctcatggcgc gactcaaggt tgaaatgggc aaaatccagg aggcctaccc cctctcgacc
     4861 aacctcacgg cctcgatccc cgcatccatt ccactcgagc ggcgcgccgc ggcgctgctc
     4921 atcgaggccg acctgctcga ccggatggga caggcagatt cggcgctgca ggcctacgag
     4981 cgcattctgg ccgaaagccc ggccggcccg ctactcgaac aggccatcgc gcgctacatc
     5041 gcactgagcc tgcgggcgcc ggacatggcg accccggttc agcggttcga ggctgtgctc
     5101 gcggcgcacc ccaacaatgc agccctcgcc ccggtccgcc tgacgctggg cgaactcaaa
     5161 ctcaagcagt attacgcgtc caggacggcc gcggcgcctg gggctccggc gaacctggcg
     5221 cttgccacga acctcttggg acaggccctt gcccagttcg attgggtggc gactaatcgt
     5281 cccgcaggcc cgttgttcgg ccgcgcccag ctcaatcggg gatggtgtca ttgggaagaa
     5341 ggtccgcaga gcctggctga aggcctcagg gcgttccggg acgccgcggc gcacttgtct
     5401 tcgccggctg accagaatat tgcgcggttc aagtgggccg actgtcagct tcggctgtcg
     5461 gacatcccag gagcgatatc caactactgg atcgttgcca ccaatgctcc ggcaaccgca
     5521 ggcgctacca acgatttggc gacggaggcc ctggcccaaa tcgtccgcgc aggcgtcgag
     5581 gctggcgatc tggccaacgc ctcgaccgcc ctgaaccacc tgttgcgcgc ggacccgacc
     5641 ggacaactcg ccgatcgccc gactttgttg gtgggccagg ccctcgcaca gttcggccag
     5701 ccccaggccg ctcggacgct ctacgatgaa ttcctgcgac gttacacaaa ctcggcgcgc
     5761 ctcccggaaa tccgtctggc cctcgccgag acattcgaac tggagcgcgg atgggaggcc
     5821 gctcaccgag cgtatgcagc ctggttgggc gcctacagca acaaccccgc ggtctccacc
     5881 aacatgatcg cccaggccac gttcgacctg gcgcgcgtcg cctaccgcgc ccagcccgac
     5941 accaacgcct tggcgcttct gacgaatttc gtccgcctct atccgtatca caccaacgcc
     6001 ccgctcgcgc agtatctggt cggcgaatat gccttcgggc agggcgatta cgcgggagcg
     6061 gagttgtatt tcctggatcg atcgctcacc cagaacacga acctcctctg gggcgaactc
     6121 acctatcgcg cccggttgat ggccggacgc gccgccgtcg cccgccagag ctaccgcagc
     6181 gctcgggacc acttcgattg gctgatcacc aacgggccgc tccacgtggc caactcgccc
     6241 gtccccattc caatcgtggc ggaggcctat ttgttccgcg gcgacacctt cgtcctggaa
     6301 ccgactgaag ccgagaccaa cagtctggcg cgctttggcg aagccatcaa cgccttctcc
     6361 aaaatcacgg agcggttccc gaccaacgag ttcgctcccc tcgcctgggg ccggatcggc
     6421 gagtgccact tccaactcgc cactctggac cccaaacggt acgacagcgc cgccaatgcc
     6481 tttcgacggg tgctcgaatc gccggccgac gcctccctgc ggagccaggc cgagtggaaa
     6541 ctcggcgtgg tcctcgaaaa gcaggcccag ctcaagcccc cccccgagcg cgccgccctg
     6601 tccgagcagg cgctcgatca ttacctccgc gtcctctatg ccaagaacct ccggcccgac
     6661 gagcagcccg atccgtactg gctcaaacgc gctggcctca gcgccgccga actggccgag
     6721 agcctcaaga agtgggatgt ggccctcgga ctcacccgcc gcctgatcac cgaccttccg
     6781 atcctcagac cccgcctcga aaaaaagatc gaggatctcc aagccgcccg acagagcggc
     6841 gacaccccgt agcgcgaaag ggatgcactc gagtcgtcgt tcccgtcaac gccaggctgc
     6901 accattgact gtggattatc aatcctgcag gccgcgcgcc ctcggactgc atggggcatg
     6961 catcacccct gctacagcga ggcagcggtc attggaaacg cgcattttgg atggcacatg
     7021 ccacaccaat gacagggagt gagtccattc tgcaatacgt gatcgtgttc atgaaatggc
     7081 ggtttcttag cccccgccaa cgcagcgcga tgaccggccc atgcgtcggc atcgccggtc
     7141 catttcgcgg catttcgcgc tatgcgcacc cgctcgccgc cattaccatg cgcgaaagaa
     7201 aacagtggaa tccatgaaca tcgaaaaacc gatcacgacc tcagacacgc cagccaggct
     7261 cggcatcgtt ggacgccgcg ttgggagaca tctggcggga ctcactctgc tcgcggtgct
     7321 gacgatcctg cccgatgccg tccaggctca gttcctttac acgaccgatc ggaaccgaat
     7381 cacgatcacc cggtacaccg gcccgggcgg tgccgtgacc attcccgaga ccataaacgg
     7441 cctgcctgtg gtcggcatcg cggacaaggc gttcgagggg agcgtcgaca tgaccagcgt
     7501 cacgatcccc gcctcggtga cgaccattgg cgcctgggtg ttcaagggat gcacgagcct
     7561 ggccgccatc gaggtgcgca catcgaacac ggctttccgc agcatcgaag gcgtgctgtt
     7621 caacaagatg cagacgaaac ttctggcctg tccgggagcg cgggaaggcg cctacgccgt
     7681 ccctggatcc gtcaccagca tcgagacagg ggcattccac ggctgcgctc gcctgaccag
     7741 cctgacgatt cccaggaatg tcaccgccat ccgggccggg gcattcgggg gctgcactgg
     7801 cctgacgagc gtcgcgctcc cggagcgcct gacgaagatc gaggccgggc tgttcgaagg
     7861 ttgcgtcaac ctgaccagcg tcgcgattcc caccacggtc acccttgtcg ggagcggcgc
     7921 gttccgagcc tgcacgggcc tgaccgccat cacgatcccc aacgccg
//
LOCUS       DAOQRO010000138         7912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8767_length_7912_cov_4.793305, whole genome shotgun sequence.
ACCESSION   DAOQRO010000138 DAOQRO010000000
VERSION     DAOQRO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8767_length_7912_cov_4.793305"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4485
                     /locus_tag="PKK20_06675"
     CDS             <1..4485
                     /locus_tag="PKK20_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNU99605.1"
                     /translation="FIPAAVLTNVGSYDVIVRNAHGSVTSQIASLTLTFPSPAQQTYE
                     PPGPSSRRTGIAITEIMYHPAPRSDGRNLEFIELYNSNPFFEDISGWRFSGECDYTFP
                     PNTILAGNSYLVVAAAPDDLRAVSGATNVLGPFDGKLANEGGTIRLRKRSGAIVLEVS
                     YADRPPWPVAPDGTGHSLVLARPSHGERDSRAWASSAARGGSPGRQDPLPVDPLAGIV
                     INELLGHADPGQPDFIELYNGSPRPADLAGCYLSDDRIALKFRIPDGTVLDPGGFVVF
                     DETQLGFALDAAGEAVFLSHPALDRILDAVRLTPQWQGAAFGRFPDGAERLRTLDVPT
                     PGTTNARPRRSAVVINEIMYHPISEMDDDQYVELHNSGPDGVDLGGWRLADGIEFRFP
                     PGTSVAPGGFLVVARNAVRLRANHPDVDPALILGDFTGALSGRGERIGLEAPLGFDGA
                     ASPPEEPASEPVWVLIDEVTYRDGGRWGRWADGGGSSLERIDPRADADLAPNWADSDE
                     TAKAPWTTVEATGPLVSPHPSTPSAEQLQLFLMGAGEALVDTVQVLVAGNNRIANSTF
                     ENGLDGWTFQGTQSHSRWEKTEGHQSARSLRLVATERGDHVVNRAFTRLQSSIPVGTS
                     VTIRARVRWLKGHPEILLRLRGGALEAYGRLNLPHNLGTPAARNSQSRANAPPAITDV
                     AHRPVLPASGQPIRVTARLSDPDGIAGAFVHYRIDPDLATTAVAMSDDGAGCDEFPKD
                     GVYSAILPAQPKGTLIAFFVEALDAHAAPATSRFPADAPARECLVRVGEDHVPGAFAN
                     YRLWITAASHAHWASREKMSNDDVDATFVYGTNRVIYNAGAHYSGSSYTSPGYTSPTG
                     NLCGYDVNFPADDRFLGERHVTLDWPVRDDTNQREQLMYWFLDQYGLPNMYRRYVHLF
                     VNGVRRGAIYDDVQQPGRDTIEEWFPGDDRGDLYKTDCWNEFDNAGNRLDPCILNTLE
                     KFTLPDGRANTARYRWNWRPRAVRGSANDFAPLFALVDAVNATNDYIGRVEAIVDVDH
                     WMLTFAMNDLASFWDAFGNPNAKNTFLYKPERDSWKLMCWDFDVGLGVFNDPPDAPLF
                     DVGDPAIRRMYQTPAFVRRYWAGLHEAMQTFFRASAIDPILDAKYAAFRANNVALNSP
                     DAIKSWIRRRRAYLEAQLKTVEAVFAITTNNGNDHTTSQTVVSLTGTAPVWVRAITVN
                     GIPYPLTWFSPTRWSLRLALQPGANAIEVQGLDRAGQPVADAVDSIRIECSSTNTPPP
                     ALFINEWMASNRGAVADPADGAFDDWFEIHNPGPIPISIAGFWLSDDPATPSLFVVPQ
                     GFHVPPHGYLLVWADGQPEQSRPDGDLHVNFRLDQLGERILLADATGRPIDEVVFVAQ
                     ETDRSEGRWPDAAPLPFYRMSPPTPGAPNSIASTEWPKIRIYEVRSDPAAGVTLTWEA
                     LPGRTYRVRYCEDLENGCWTELPVEVQAQNAVASLTDPAVEGSERRYYQVVLRTGVSP
                     "
     gene            4956..7880
                     /locus_tag="PKK20_06680"
     CDS             4956..7880
                     /locus_tag="PKK20_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99606.1"
                     /translation="MRSQQPQIRTAGYSAMTLPDTLEELKPYTVKAVTLLAVWFAACG
                     AIRPCNAQELAGRRIPAPLREWQDWATWDLKQRNCPTPFNDAARRVCFWPGALSLAAD
                     QKSATFELSVAVFSRTWVPLPGGGETWPLEVRANGAPIAVVEHENRPCLELEPGRHQI
                     AGSFRWETEMPQLIRVPREVGLLSLVLEGKKVELPNWDEAGDLWLKRVRLEATDKDTV
                     AAQIWRVIEDGIPLWLRTELELSVSGKSREEDLGHIVPEGWSIGSVDAPVPVAVDDQG
                     RMKAQVRAGKWTIRMDAFRTTDAGEIRFAQGARPIVATESVGFRAAPEFRMAELDGIE
                     VVDVSQTAFPRKWRNLPVYQWPTDQAFRLVEKMRGMGLQRPEGLKISRVFWLDEDGNG
                     LTFRDQIHGTMQQIWRLDVAVGQKLGAAKMGGQPQLITVDPVNGTAGVEIRTRNLDLQ
                     AIGRSDRMKAIPATGWQAPADELDVSMNLPPGWRLLALFGADWVEGDWLTAWTLLDLF
                     LLLIFSLAVFKLWGWKAGLVAFLAFSLGYHEPGAPRLTWLFMLLPLALLRVVPQGGVR
                     KAIIGWQWLAVALLLFGLVPFVASQLQSVIYPQLERQGFSYGSYPLFGWRAYHPPPQW
                     AAPRQAAQVPEAAQAQQAEVPALGKRAARYGLESNLAYESKAKIQTGPAEPEWNWNLV
                     RCGWNGPVSASETIRPVLISTSVHRVLTVLRVALLLALGGLLLRRGGWKPIAVGPGKA
                     TGLGAAAALLLALCVPTSSLAQVPDKETLNTLRERLLETSDAYPRAAEIPTVDLNVTD
                     GRISMTVEVHATAQVAVPLPGKLPSWSPVSVQLDDKPEAVLRRDDGYLWIVASAGVHR
                     VVVEGFLPSATEWEWTFLLKPRRVAIDAPGWTVTGVRPNGIPENQVFFARQRETAAGE
                     AEYDRKDFNAIVAVERNLEVGLVWQARTVITRLAQTGKAVSLRVPVLEGEKVLSLAAC
                     RT"
BASE COUNT         1395 a         2656 c         2460 g         1401 t
ORIGIN      
        1 ttcatccccg ccgccgtcct gaccaacgtg ggcagttacg acgtcatcgt ccgcaacgcc
       61 cacggctccg tcacaagcca gatcgcctcc ctcaccctca ccttcccaag tccggcgcaa
      121 cagacatacg aaccgcccgg cccttcgagc cgccgaacgg gcatcgccat caccgagatc
      181 atgtatcatc ccgccccgcg atccgacggg cggaatctcg agttcatcga actctacaac
      241 tcgaatcctt tcttcgagga tatcagcgga tggcgctttt cgggcgaatg cgactacacc
      301 tttcccccca acacaatcct ggccggcaac agttatctcg tcgtggctgc cgctcctgac
      361 gacctgcgcg cggtgtccgg cgcaaccaat gtgctgggtc cattcgacgg caagctcgcc
      421 aacgaaggcg gcaccatccg cctccgcaaa cggtccggcg ccatcgtcct cgaggtctct
      481 tatgcggacc gtcccccctg gcccgtcgct ccggacggca cgggtcactc gttggtcctg
      541 gcccgcccga gccatggcga gagggactcg cgagcctggg cttcgagcgc ggccaggggc
      601 ggctcgccgg ggaggcagga cccgctcccc gtggacccgc tggccggcat cgtcatcaac
      661 gaactcctcg ggcatgccga cccaggccag cccgacttca tcgaacttta caacggcagc
      721 ccccgcccgg ccgacctcgc cggatgttac ctgagcgacg atcgcatcgc actcaaattc
      781 cgcatccccg atggcacggt gctcgacccg ggcgggttcg tcgttttcga cgagacccaa
      841 ctcgggttcg ctctggatgc agccggggaa gcggtgttcc tgagccaccc cgcgctcgat
      901 cgcatactcg atgccgtccg tctcaccccg caatggcagg gcgcggcctt cggacggttc
      961 cccgacggcg ccgagcgcct gcgcacgctt gatgtgccca cgccaggcac gaccaatgcg
     1021 cgtccgcgcc gctcggccgt cgtgatcaat gaaatcatgt accaccctat ttcggaaatg
     1081 gacgacgacc aatacgtcga actccataac tctgggccgg acggagtcga tctgggcgga
     1141 tggcgcctcg ctgatggcat cgagttccgc tttcctcccg gcacgtcggt cgctccgggt
     1201 ggttttctgg tggtggcccg caacgccgtc cgcctccggg ctaatcaccc cgatgtcgat
     1261 cccgctctca tcctcgggga cttcaccggc gcgctctctg gtcgaggcga gcgcatcggg
     1321 ctcgaagcgc ccctgggatt cgatggcgca gcttcaccgc cggaggaacc ggcctccgaa
     1381 cccgtctggg tgctgatcga tgaggtcacc taccgcgatg gcggtcggtg ggggcgctgg
     1441 gcggacggtg gcggcagcag ccttgaacgg atcgaccctc gagccgacgc cgatctggct
     1501 cccaactggg ccgacagcga cgagaccgcc aaggccccct ggacaaccgt cgaggcgaca
     1561 ggtccccttg tctcgccgca tccgtcgacg ccctccgccg aacaactgca gctcttcctg
     1621 atgggtgcgg gcgaggcgct cgtcgacacg gttcaagtgc tcgtcgccgg caacaatcgg
     1681 atcgccaact cgaccttcga aaacggtctt gacggctgga cattccaggg cactcaaagc
     1741 cacagcaggt gggagaagac cgaaggccat cagagcgcac gctcgctgcg gctcgtggct
     1801 accgaacgcg gagatcacgt cgtcaaccgc gccttcaccc ggctccaatc gagcatcccc
     1861 gtcggaacct cggtcacgat tcgcgcgcgc gtccgctggc tcaaaggaca tcctgaaatc
     1921 ctcctgcgcc ttcgcggagg cgcactcgaa gcctatggcc gcctgaacct cccgcacaat
     1981 ctcggcaccc ccgccgcacg caacagccag agccgcgcca atgcacctcc cgccatcacc
     2041 gacgtcgccc atcgtcccgt cttgccggca tccggacaac ccatccgcgt cacagcccgc
     2101 ctcagcgatc ccgatgggat tgccggcgca ttcgtccact accgcatcga tcccgatctc
     2161 gctaccacgg cggtggccat gtccgatgac ggcgccggat gcgacgaatt cccgaaggac
     2221 ggcgtctatt cggccattct ccccgcccag ccgaagggaa ccctcatcgc gtttttcgtc
     2281 gaggccctcg atgcccacgc cgcgcccgcg accagccggt tccccgccga tgcgcccgcg
     2341 cgcgaatgcc tcgtcagggt cggcgaagac catgtgccgg gcgctttcgc caactatcgc
     2401 ctctggatca ccgcggcgtc ccacgctcat tgggccagcc gcgagaaaat gagcaacgac
     2461 gacgtcgacg ccacgttcgt ctatggaacc aaccgggtca tctacaatgc cggcgcgcat
     2521 tacagcggca gttcctacac ctcccccggc tacaccagcc ccacaggcaa cctctgcggg
     2581 tatgacgtca actttcccgc cgacgaccgg tttctcggcg aacggcatgt cacgctcgac
     2641 tggcccgtgc gcgacgacac caaccaacgc gaacaactca tgtactggtt cctcgatcag
     2701 tacggcctgc ccaacatgta ccgccgctat gtgcacctgt tcgtcaacgg cgttcgacgc
     2761 ggcgccatct acgacgatgt ccagcagccg ggcagggaca ccatcgagga atggttcccc
     2821 ggggacgacc gcggcgacct ctacaagacc gattgctgga acgagttcga caacgccggc
     2881 aaccgcctcg atccgtgcat ccttaacacc ctcgaaaagt tcaccctgcc cgacggccgc
     2941 gcaaacaccg cccgttaccg ctggaattgg cgcccgcgcg ccgtgcgcgg gtccgccaac
     3001 gacttcgcgc cgctcttcgc cctggtcgat gcggtcaacg ccactaacga ctacatcggg
     3061 cgcgtcgagg cgattgtgga cgtggatcac tggatgctga ccttcgccat gaacgacctg
     3121 gcttcattct gggatgcatt cggcaatccc aacgccaaaa acaccttcct ctacaagccc
     3181 gaacgcgatt cctggaaact gatgtgctgg gacttcgatg tcggcctcgg cgtattcaac
     3241 gacccgcccg acgcgccgct gttcgacgtg ggcgaccccg ccatccggcg catgtaccag
     3301 acgcctgcgt tcgtccggcg ctattgggcc ggcctgcacg aggccatgca gacgttcttc
     3361 cgggccagcg ccatcgatcc catcctcgac gccaagtacg ccgcgttccg cgccaacaat
     3421 gtcgccctga acagcccgga cgccatcaag tcctggattc gccggcgccg cgcgtatctc
     3481 gaagcccagt tgaaaacggt cgaggccgtc ttcgccatca cgaccaataa tggcaacgac
     3541 cacacgacgt cccaaaccgt cgtctccctc accggcaccg cccctgtctg ggtccgtgcg
     3601 atcacggtca acgggattcc ctaccccctg acctggttct cacccacgcg ttggagcctc
     3661 aggctagccc tgcaaccggg cgccaacgcg atcgaggttc agggactcga ccgcgccgga
     3721 caacctgtcg cggacgccgt cgactcgatc cgaatcgagt gctcgagcac gaacacgccg
     3781 ccccccgctc ttttcatcaa tgaatggatg gcctcgaacc ggggcgccgt ggccgatccc
     3841 gcggacggcg cgttcgatga ctggttcgaa atccacaatc caggccccat cccgatctcg
     3901 atcgccggat tctggctcag cgacgatccg gcgacaccgt ctctgtttgt cgttccgcag
     3961 gggttccacg tcccgccaca cggttatctc ctcgtctggg ccgacgggca gcccgaacag
     4021 tcacgccctg atggcgacct gcacgtgaac ttccgactcg accaactggg ggaacgaatc
     4081 ctgcttgcgg acgccacggg gcggcccatc gatgaggtgg tcttcgtcgc gcaggagacc
     4141 gaccgcagcg aggggcgctg gcccgacgcc gccccactgc ctttttaccg gatgtccccg
     4201 cccacaccgg gcgcaccgaa cagcatcgcg tccaccgagt ggccgaagat tcggatttac
     4261 gaggtgcggt cggatcccgc ggcaggcgtg acgttgacat gggaggcttt gcccgggcgc
     4321 acctatcgcg tgcggtattg cgaggatctc gagaacggtt gctggaccga actgccggtc
     4381 gaggttcaag cccagaatgc ggtcgccagc ctgaccgacc cggccgttga ggggtcggaa
     4441 cggcgttatt accaggtcgt cttaagaacc ggggtcagcc cttaagttac aatgtaagcc
     4501 tggcacgcaa tgagagggag cgtgtccaaa ttgccgtggt gcccgcggcc gtcaagttga
     4561 acaaggtcgc ggtgtgtgtc ggcgatttga agtccgccac cggcgcaatg ttcacgcgcg
     4621 cgcaggttga gtgtgctgcg gtcggttatg tcgcgaccaa gacgcgcccg ccctatggta
     4681 cgtcgcacat cggttggata aagtgacagg ttcttcatcc tgtttccaac ctggacaaca
     4741 gcgacccttc agggatttaa ctgaaatgac cgtgccggcg gcttgggccg attcaaagaa
     4801 tgcttgaccc cttcgacgca gcacacacac tgagcttgct cgatacgagc acgggcttga
     4861 ccggtcacgg attcaacgag caaagagcaa ttcatcgatg accagcccaa ccgctgtggc
     4921 gcgggtcgtt ttactgtgct catgcctggc ggaggatgcg cagccaacag ccgcagattc
     4981 gcaccgcggg ttattccgcc atgaccctgc ctgacacact cgaggaactg aagccctaca
     5041 cagtgaaagc agtcaccttg ctggccgtct ggtttgccgc ctgcggcgca atccgtccgt
     5101 gcaacgccca ggaactggcc gggcgccgaa tccccgcgcc gctcagggag tggcaagact
     5161 gggcgacctg ggacctcaaa cagcgcaatt gtccgacgcc gttcaatgac gccgcccggc
     5221 gcgtttgttt ctggcccggc gcgctgtcgt tggcggcgga tcagaaatcc gcaacgtttg
     5281 aattgagtgt ggccgtattc agccggacct gggtgccgct gccggggggc ggggagacct
     5341 ggccgctcga ggtgcgcgcg aacggcgcgc cgatcgccgt tgtggagcac gagaaccggc
     5401 cctgcctcga gctcgagcca ggacgtcatc agattgcggg atcgttccgc tgggaaactg
     5461 aaatgcccca actgatccgc gtgccgcgcg aggtaggcct gctttcactc gtcctcgaag
     5521 gcaagaaagt cgagctgccc aactgggacg aggccgggga cctctggctc aaacgcgtcc
     5581 gtctcgaggc tacggacaag gacacggttg ccgcccagat ctggcgcgtc atcgaggatg
     5641 gcattccgct ctggctgcgc accgaactcg agttgagcgt gtcggggaag agccgcgagg
     5701 aggacctggg ccatatcgtg cccgagggtt ggagcatcgg gtcggtggac gcgccggtgc
     5761 cggtggccgt ggacgatcag ggccgcatga aggcgcaggt gcgggcgggc aaatggacca
     5821 ttcgcatgga cgcgtttcgt acgaccgacg caggtgagat taggttcgcg caaggcgccc
     5881 gaccgattgt ggcgacggag tcggtgggct ttcgggctgc accggagttt cgcatggcag
     5941 aactcgatgg catcgaggtg gtggacgttt cgcagacagc ctttccgcgg aagtggcgaa
     6001 acctgccggt gtatcaatgg ccgacggacc aggcgttccg gttggtggag aagatgcggg
     6061 ggatgggact gcaacggccg gaggggttga agatcagccg ggtgttctgg ctcgatgagg
     6121 atggcaatgg gctgacgttc cgcgatcaga ttcatggcac gatgcagcag atctggcggc
     6181 tcgatgtcgc ggtgggccag aaactcggcg ccgcgaagat ggggggacag ccccagttga
     6241 tcaccgtcga cccggtcaac ggcaccgcag gcgtcgagat tcgcactcgg aacctggacc
     6301 tccaggccat cgggcgttca gatcggatga aagcgatacc ggccacgggc tggcaggcgc
     6361 ccgcagacga gctggatgtc tcgatgaatc tgccgccggg ctggcggttg ttggcgttgt
     6421 tcggcgcgga ctgggtcgag ggcgactggc tgaccgcgtg gacactgctc gacctgttcc
     6481 tgctgctgat cttttcgctg gccgtgttca agttgtgggg ctggaaagcg gggctggtgg
     6541 cattcctggc cttcagtctt gggtatcacg agccgggcgc accccggctg acgtggctct
     6601 tcatgttgtt gcctttggcg ctgctgcgcg tggtgcctca aggcggcgtg cgcaaggcaa
     6661 tcatcgggtg gcagtggctt gccgttgcgc tgttgctgtt cggtctcgtg ccattcgtcg
     6721 ccagtcagct tcaaagcgtc atctatccgc agctcgaacg gcagggattc agctacgggt
     6781 cgtaccccct cttcggctgg cgcgcgtatc atccgccgcc tcagtgggcc gcgccacgcc
     6841 aggccgccca ggtgcccgag gctgcgcagg cgcagcaagc ggaagtgccc gcgctcggaa
     6901 agcgggccgc tcgctatgga cttgaatcca acctggccta tgaatccaag gccaagatcc
     6961 agaccggccc cgccgagccg gaatggaatt ggaacctggt ccgctgcggt tggaacgggc
     7021 cggtatcggc cagtgaaacc atccgccccg ttctcatctc gaccagcgtg catcgcgtgc
     7081 tgacggtgtt gcgcgtggcg ctgctgttgg ctctgggcgg cctcctgctg cggcgaggcg
     7141 gttggaaacc gattgccgtc ggcccgggca aagccaccgg tttgggggcg gcggcggctc
     7201 tcctgttggc actgtgcgtg ccgacgtcgt ccctggctca ggtgccggac aaggagacgc
     7261 tcaacacgct gcgcgagcgc ttgctcgaga catcagacgc ctatccgcgc gccgcagaaa
     7321 tcccgactgt ggatttgaac gtcaccgacg gcaggatcag catgacggtt gaagttcatg
     7381 cgacagcgca ggtggccgtg ccgttgcccg ggaaactgcc ctcctggtcc cctgtgtctg
     7441 tgcaattgga tgacaagccg gaagccgtct tgcgccggga tgacggctat ttgtggatcg
     7501 tcgcgtccgc gggcgtccat cgtgtcgtgg tggaagggtt tctccccagc gccacagaat
     7561 gggaatggac gttcctgttg aaaccgcgcc gtgtcgccat tgacgcccct ggctggacgg
     7621 tcacgggcgt gcgcccgaat ggcatcccgg aaaaccaggt cttcttcgcc cgccagcgcg
     7681 agaccgccgc cggcgaagcc gagtatgacc gcaaggattt caatgctatc gtggccgtgg
     7741 aacgtaacct ggaggtcggg ttggtctggc aggcgcgcac cgtaatcacc cgtttggccc
     7801 agacaggcaa ggcggtctcc ctgcgcgtcc cggtgctcga gggcgagaaa gtgctctcgc
     7861 tagcagcctg tcggacttag atcgagaagc aagtgagggt gatggtggta gg
//
LOCUS       DAOQRO010000139         7893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8803_length_7893_cov_3.923833, whole genome shotgun sequence.
ACCESSION   DAOQRO010000139 DAOQRO010000000
VERSION     DAOQRO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8803_length_7893_cov_3.923833"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKK20_06685"
     CDS             <1..1057
                     /locus_tag="PKK20_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNU99607.1"
                     /translation="FLESGFLAVLLFGWDKVSRGMHFFATCMVCLGAHFSAVWIVVAN
                     SWQQTPAGYMIEQTALGPRAVITDFWAMVFNPSSMERLSHVVCGAWQAGAFLIISVSA
                     FYLLRRKHEEFAKASMKVGLGAAVVASILQIVTGHQSALGVIRNQPVKMAAFEGHYET
                     GELPIYAFGWVDEASETVRGLAVPRAVSRTMLGKAGSTAKGLREFPPEDRPRVNLTFQ
                     FYHLMVGVGMALMAVALAGSFLCWRGALSRSRWLLWIMVFSVLGPQIANQTGWFAAEM
                     GRQPWVVYGLLRTSDGLSAVVKADAVLTSLILFTLVYLLLFAVFVHLLNEKIRHGPDP
                     SDMKPGGKLASGGRADL"
     gene            1054..2088
                     /gene="cydB"
                     /locus_tag="PKK20_06690"
     CDS             1054..2088
                     /gene="cydB"
                     /locus_tag="PKK20_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNU99608.1"
                     /translation="MNFPDWLDLNLVWFVLVGVLFTGYALLDGFDLGVGALHLFTRTD
                     PHRRLMLNSIGPVWDGNEVWLVTGGGALFAAFPVVYATVFSGFYLALILLLVALIFRA
                     VAIEFRSKQPMAWWRRMWDVGFAAGSVLSSFLLGVAMGNIAWGMPLDERGEFAGTFLG
                     LLHPYALLVGVTAVALFMMHGAIYVVMQTEGDLQAQARQWVRPTMIFFILCYAVTTMA
                     TLLYAPHMAARMRANPWLFTIALANVLAIANIPREIHHGRDFRAFLSSGCAMIALMML
                     FGLGLFPNLVPSNPDPIRSLTAYNASSSPKTLGIMLTIAIIGVPAVLAYTVSIYWIFR
                     GKVKLDRMSY"
     gene            2127..2363
                     /locus_tag="PKK20_06695"
     CDS             2127..2363
                     /locus_tag="PKK20_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99609.1"
                     /translation="MEVEYIVIAGWPVQFLSFDWLITNGPLRLANSPIPIPIVAEALA
                     NGLEFLQGHSRSHNDRRGADGSEELAIGYCAPKQ"
     gene            complement(2342..3457)
                     /locus_tag="PKK20_06700"
     CDS             complement(2342..3457)
                     /locus_tag="PKK20_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU99610.1"
                     /translation="MNAAFLAAAIALTPASSAAGATNDWPMYRGGPALLGVSQGSLPD
                     ALARRWQFKTEGPVKSSAAIVGGRVYVGSDDGHLYSLDLATGRKVWSFKTDGPVESSP
                     LVHEDRVFFGSSDNSLYAVGARDGRLAWKYETEDRILGAPNWVKAPTGQGVWILAGSY
                     DFKLHCVDGATGKSNWVYETGNYINGSPAVAAGQTVFGGCDAILHVIGLTKGEQVKGV
                     EAGAYIAGSVALEGARAYFGHYNNEFLCVDIEKGERVWTYRDRNFPYFASPAVTEDKV
                     VFGGRDKRLHCVSKSEGKPLWTFATRGKVDSSPAVAGHKVVVGSDDGRLYIVSIAEGK
                     ELWHYEIGQAITASPAVAGGLVVVGSEDGNVYCFGAQ"
     gene            3569..4003
                     /gene="rplM"
                     /locus_tag="PKK20_06705"
     CDS             3569..4003
                     /gene="rplM"
                     /locus_tag="PKK20_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNU99611.1"
                     /translation="MKTYLPKVNLDARKWHVVDAEGKVLGRLAVEVANVLRGKNKPIY
                     TPHLDAGDFVVVINAEKVVLTGKKETDKEYMSYSGWKGGEKRQTVAQVRARYPERLIL
                     HAVKGMIPKNRLGAVLLTKLKVYKGNQHPHAAQQPAPMTINN"
     gene            4011..4406
                     /gene="rpsI"
                     /locus_tag="PKK20_06710"
     CDS             4011..4406
                     /gene="rpsI"
                     /locus_tag="PKK20_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNU99612.1"
                     /translation="MSEVTEFLGTGRRKTAVARVRLAPGTGKITVNGRTFEKYFVVET
                     HRAFVMQPLLATESTAKFDVRVNVIGGGPNGQAGAVRHGISRALLKADSAFRPILKAQ
                     GLLTRDPRMRERKKYGQPGARKRFQYSKR"
     gene            4527..5909
                     /locus_tag="PKK20_06715"
     CDS             4527..5909
                     /locus_tag="PKK20_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99613.1"
                     /translation="MNTPLTRRGFLRANLTFTFAAATLPHLVPASVLGQGQPSPNSRI
                     QVGCIGVGPQGRGVMSNFLSQPDCRVIALCDVAKRNLDAALQMVSGHYKDQACAKHHD
                     YRELLRRSDIDAVLIATPDHWHVPIAVAAARANKDMYVEKPLGLSVEEDQRLRAVCGL
                     TKRIFQFGTQQRSGSQFRLACELVLNGRIGSLKEISVWASASRPGGSTRPVPPPADLD
                     YPQWLGPARAMPYTDGKAFDGDPADTWKTWWFNYDYALGFIAGWGVHPLDIAYWGHPA
                     MMRGKVGVEGKGVIPTEGACNTSVAWDVRFKFADGVRMIYRGTRNNYEPVTPMNDLRP
                     WEQKFGRAIDHGTAFEGTDGWVLVDRGAIRTSPEDLVETTFGPNDRRLVQSSNHVRNF
                     LDSIKSRQPAVSPIEDSVQADILCHLSDIATRVDRPLNWDPAKEQFVDDNDANARLKM
                     RPVRQGWEWA"
     gene            complement(6019..7155)
                     /gene="mnmA"
                     /locus_tag="PKK20_06720"
     CDS             complement(6019..7155)
                     /gene="mnmA"
                     /locus_tag="PKK20_06720"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNU99614.1"
                     /translation="MSDRLSTRVVVGMSGGVDSSTAAILLRNEGYAVVGVTLKLWSPE
                     AMPGAQERFSRRSDAATAEDARAVCDRLGIPFHVVDGADAFRQRIIQYFADEYRAGRT
                     PNPCAMCNARIKFDLLLSCARRLGAHQIAFGHYARVERNEASGRWLLRRGRDSRRDQS
                     YFLFSLNQEQLAHALLPLGDLLKSQTREIARAHQVAAVEKAESMEICFVPDNDYGRFL
                     REARLVAPHPGDIVDLHGRVLGRHEGIEFYTIGQRRGLRIAASAPLYVVDLDAAGNRV
                     VVGDSTSLEQSEFMIERCNWIPFEAPPAQLDVMAKIRYNHPGTTATVVAHANGTARVR
                     LDIPQRAITPGQACVFYQDDLVIGGGWIARAGSPSPDRGSGTRA"
     gene            complement(7203..7802)
                     /locus_tag="PKK20_06725"
     CDS             complement(7203..7802)
                     /locus_tag="PKK20_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNU99615.1"
                     /translation="MKTGLHRDRTFALGGAAVCLVLLLIALLALWRRASERLDHSQDA
                     PILEAARRYKLDPALVKAVVWRESRFHPGVRGRAGEIGLMQLTDAAAQEWAETVRAYP
                     LPEAHLYDPRTNAFAGTWYLCKLIERYRKTDDPVPYALADYNAGRANVLKWAREAAAT
                     NSVEFVSQIAFPSTRHYVESVIRRWRYYQQKTGSGLSTR"
BASE COUNT         1398 a         2498 c         2485 g         1512 t
ORIGIN      
        1 ctttctcgag tccgggtttc tggcggttct tctgttcggc tgggacaaag tcagccgcgg
       61 catgcatttt tttgcaacct gcatggtgtg tctcggggcg catttcagcg ccgtctggat
      121 cgtcgtggcc aactcgtggc agcagacgcc ggcgggttac atgatcgagc agacggcgct
      181 ggggccgcgg gcggttatca ccgatttctg ggccatggtg ttcaatccgt cgtcgatgga
      241 acggctgagt cacgtggtgt gcggggcgtg gcaggcgggg gcttttctga tcatcagcgt
      301 gagcgccttc tacctgttgc ggcgaaagca cgaggagttt gcgaaagcct cgatgaaagt
      361 cggcttgggc gcggctgtcg tggcttcgat cctccagatc gtcaccgggc accagagcgc
      421 gctgggtgtg atcaggaacc agccggtgaa aatggcagcg ttcgaggggc attacgagac
      481 gggggaactg ccaatttacg cgttcgggtg ggtggatgag gcgtccgaaa ccgtccgagg
      541 ccttgcagtt ccgcgcgctg tgagcaggac gatgctgggg aaggccggat cgacggcgaa
      601 gggactgcgg gaattcccgc cggaggatcg tccgcgcgtg aatctcactt tccagttcta
      661 tcatctcatg gtcggagtcg ggatggctct gatggccgtg gcgctggccg gttcgttcct
      721 gtgctggcgg ggggccttgt cgcgatcccg ctggctgctg tggattatgg tcttctcggt
      781 tctggggccg cagattgcga atcagaccgg atggtttgcg gcggagatgg ggcggcagcc
      841 ttgggttgtc tacggcctgc tgcggacttc ggacggcctg tcggccgtgg tcaaggccga
      901 tgccgtgctg acctcgctga ttctgttcac gctcgtttac ctccttctct ttgccgtgtt
      961 cgtgcacctg ttgaacgaga agatccggca tgggccggac ccgtcggaca tgaagcccgg
     1021 gggcaagctg gcgtccgggg ggagggccga cctatgaact tcccggactg gctggatctc
     1081 aatctggtgt ggtttgtcct ggtgggcgtg ttgttcaccg ggtacgcatt gctcgatggc
     1141 ttcgatctgg gggtgggcgc gctgcacctc ttcacgcgca ccgatccgca tcggcggctc
     1201 atgctcaact cgatcgggcc ggtctgggac ggcaacgagg tgtggcttgt gacggggggc
     1261 ggggcgttgt tcgcggcgtt tccggtggtc tatgcgaccg tgttcagcgg attttatctt
     1321 gcattgatcc tgttgctggt ggcgctgatt tttagggcgg tcgccatcga gtttcgcagc
     1381 aagcagccga tggcgtggtg gcggcggatg tgggatgtgg gctttgcggc gggcagcgtg
     1441 ctttccagct tcctgcttgg ggtggcgatg ggcaacatcg cctgggggat gccgctcgat
     1501 gagcggggcg agttcgccgg cacgttcctc ggcctgctcc atccctacgc gctcctggtc
     1561 ggagtgactg cggtggcgtt gttcatgatg cacggcgcga tctacgtggt gatgcagacc
     1621 gaaggggatc tgcaggcgca ggcgcgacaa tgggtgcggc ccaccatgat tttcttcatt
     1681 ctttgctacg cggtgacgac gatggccaca ctgctgtatg cgccgcacat ggcggcccgg
     1741 atgcgtgcca atccctggct gttcaccatc gccctggcca acgtgctcgc catcgccaac
     1801 atcccgcgcg agattcacca cggacgcgat ttccgggcct ttctatcgtc gggttgcgcc
     1861 atgattgcat tgatgatgct gttcggcctg ggactgtttc cgaacctggt cccgagcaat
     1921 ccggacccga tccgaagcct gacggcctac aacgcgtcgt cgtcgcccaa gaccctgggc
     1981 atcatgctga ccatcgcgat catcggtgtg ccggccgtcc tggcctatac cgtcagcatt
     2041 tactggatat tccgaggcaa ggtgaagctc gaccggatga gctattagcg agccgcgaag
     2101 cgcattgaat ccgccccggc ggctggatgg aagtcgagta catcgtcatc gccggctggc
     2161 cggtgcaatt cctttccttt gactggctga tcaccaacgg gccgctccgc ctggccaact
     2221 cgcccatccc catcccgatc gtggcggagg ccctggcaaa tggtctcgag tttctgcagg
     2281 ggcactcccg atcccacaac gatcgacgtg gggccgatgg ttccgaggaa cttgcgattg
     2341 gctattgcgc cccgaagcag tagacgttgc cgtcttcgct gccgaccacg accaggccgc
     2401 ccgcgacggc cggcgaagcg gtgatcgcct ggccgatttc gtagtgccac aattccttgc
     2461 cttccgcgat cgagacgatg tacaaccttc cgtcatccga gcccacaacc accttgtgtc
     2521 cagccacggc aggcgagctg tcgactttgc cccgagtcgc gaatgtccag agcggtttgc
     2581 cctccgattt cgagacgcaa tgcagacgct tgtcgcgccc gccaaacacc accttgtcct
     2641 cggtgaccgc gggcgaggcg aagtagggga agttgcgatc gcgataggtc cagaccctct
     2701 cgcccttttc gatgtcaacg cagagaaact cattgttgta atgtccgaaa taggcgcggg
     2761 cgccctccag cgcgacggac cccgcgatgt aagccccggc ctcgactccc ttgacctgct
     2821 ctccctttgt caacccaatc acgtgcagaa tggcgtcgca tccgccgaag accgtctgcc
     2881 cggcggcgac ggcgggtgaa ccgttgatgt agttgccggt ttcgtacacc cagttgcttt
     2941 tgcccgtggc tccatcgacg cagtggagtt tgaagtcgta gctgcctgcc aggatccaca
     3001 ccccctggcc ggtcggggct ttgacccagt ttggcgcgcc cagaatgcgg tcctcggtct
     3061 cgtacttcca agccaaacgc ccgtcccgcg ccccgaccgc gtaaagggag ttgtcgctcg
     3121 agccgaagaa gacccggtct tcgtgaacca gcggtgacga ttcgacaggc ccgtcggttt
     3181 tgaacgacca gaccttccgg cccgttgcca gatcgaggct gtagaggtga ccgtcatccg
     3241 atcccacata gacccgtcca cccacgattg ccgcggacga tttcaccggg ccttcagtct
     3301 tgaactgcca acggcgggca agggcgtccg gcaacgagcc ctgggagacg cccagcaggg
     3361 cgggaccgcc ccggtacatt ggccaatcgt tggtggcgcc tgcggcggaa gaggccgggg
     3421 tcaaggcgat cgctgcggca agaaacgcgg cattcattca gcgcgacagt attgtcgggg
     3481 gccggggttg gcaaacgaaa tgccgcaaat ggcagttgac ttggccggtt cgtttgctag
     3541 cctccacgcc ctttgatccg aaggcttcat gaagacgtat ttgccgaaag tgaatctgga
     3601 cgcccgcaag tggcacgtgg ttgatgccga gggcaaggtg ttggggcgcc tcgccgtcga
     3661 ggtggccaac gtcttgcgcg gcaagaataa gcccatctac acaccccatc tcgatgccgg
     3721 tgacttcgtc gtcgtgatca acgccgaaaa ggtggtgctg accggcaaga aagagaccga
     3781 caaagagtac atgtcgtact cgggctggaa gggcggcgag aagcgccaga cggtcgccca
     3841 agtccgcgcc cggtaccccg agcgcctcat cctgcacgcc gtcaagggga tgatccccaa
     3901 gaaccggctg ggtgccgtcc tgctcaccaa gttgaaggtc tacaagggca accaacatcc
     3961 gcacgccgcc caacaacccg ccccgatgac catcaataac tgaacgcatt atgagtgagg
     4021 ttaccgaatt tctcggcaca ggccgccgca agaccgctgt agctcgagtt cgtctcgccc
     4081 ccggcacagg caagatcacc gtcaacgggc ggaccttcga gaagtatttt gttgtcgaga
     4141 cccacagggc attcgtcatg cagccactgc tggcaaccga atcgaccgcc aagttcgacg
     4201 tccgtgtcaa tgtcatcggc ggcggtccca acggccaggc cggcgccgtt cgccacggca
     4261 tctcccgcgc cctgctcaag gccgacagcg cttttcgccc gatcctcaaa gctcaggggc
     4321 tcctgacccg cgacccgcgc atgcgcgagc gcaagaagta cggccagccc ggcgcccgca
     4381 agcgcttcca atacagcaag cggtaacgag gcacgcctcg catccggtcc gaatcttcag
     4441 ccaggcggca agccgcagcg tgtcaggctc ggcgccccat cattagttgc ttgcgcagat
     4501 tggccgaaga ctgtcctatt cggggcatga acaccccgtt gacccgccgc ggttttctcc
     4561 gcgccaatct cacgttcacg tttgccgcag cgaccctgcc gcacctggtc ccggcctctg
     4621 tcctgggcca gggccagcct tcccccaaca gccgcatcca ggtcggatgc atcggcgttg
     4681 ggccgcaggg caggggcgtg atgagcaatt ttctgtcgca accggactgt cgcgtgatcg
     4741 ccctgtgcga cgtcgcgaaa cgcaatctcg acgccgcgct tcagatggtc agcggccact
     4801 acaaggacca ggcgtgcgcc aagcatcacg attaccgtga gttgctgcgt cggtcagaca
     4861 ttgacgccgt cctgattgcc acgcccgatc actggcatgt gccgattgcc gtggccgccg
     4921 cgcgcgcgaa caaggacatg tacgtcgaga aaccgctcgg cctctccgtc gaggaggatc
     4981 aacggctgcg tgcggtctgt ggcctgacca aacgcatctt tcaattcggc acccagcagc
     5041 ggtccggcag ccaattccgc ctcgcctgcg aattggtcct caacggccgc atcggatcgc
     5101 tcaaagagat ctcggtctgg gcctctgcca gccgccccgg tggatcgacc agacccgttc
     5161 cgccccccgc agacctcgac tatccgcagt ggcttggacc ggcgcgggca atgccgtaca
     5221 cggacggcaa ggcctttgac ggcgatcccg cggacacgtg gaagacctgg tggttcaact
     5281 atgactacgc cctgggattc attgccggct ggggagtgca cccgttggac atcgcctact
     5341 ggggacaccc ggccatgatg cgcgggaagg tgggtgtcga gggcaagggc gtgattccga
     5401 cagagggggc ctgcaacacg tcggtggcct gggatgtccg gttcaagttt gccgatggtg
     5461 tgcgcatgat ctaccgcggc acgcgcaaca actacgagcc ggtcacgccc atgaacgacc
     5521 tgagaccgtg ggagcagaaa ttcggacgcg ccatcgatca cggcacggct ttcgaaggga
     5581 ccgacggctg ggtgcttgtc gatcgcgggg ccattcgcac atcgcccgag gacctcgtcg
     5641 aaacgacatt cggtccgaat gaccgccgac tcgtccagag cagcaatcac gtccgcaact
     5701 tcctcgactc gatcaaaagc cgtcagcccg ccgtcagccc aatcgaggac tcagtccagg
     5761 ccgacatcct ctgccatctc agcgacatcg ccacgcgcgt cgaccgtccg ctgaattggg
     5821 acccggcaaa ggaacagttt gtcgacgaca atgacgcgaa tgcgcgcctg aagatgcgtc
     5881 ccgtccgcca aggatgggag tgggcctagc tcggttattt cacacgcttc gcgaggaacc
     5941 gtaaccgtat aagaacttgg gcgggcagcc ggttggaaca ggcttttgca tttcaccatc
     6001 ccccggatgc gaagggcttc aggcacgtgt tccagaacct cgatccgggc tcggagaacc
     6061 tgcccgcgcg atccagccgc cgccgatcac cagatcgtcc tggtagaaca cgcacgcctg
     6121 cccgggtgtg atcgcgcgct gcgggatgtc cagccggaca cgcgcggttc cattagcgtg
     6181 cgcaacaacc gtggcggtcg tgccaggatg gttgtagcga atcttcgcca tgacatcgag
     6241 ttgagccggc ggcgcctcaa aagggatcca gttgcaccgt tcgatcatga actcggactg
     6301 ttcgagcgat gtgctgtcac cgaccacaac ccggttgcca gcggcgtcga ggtcgacaac
     6361 gtagaggggg gcggacgctg cgatcctgag cccgcggcgc tggccaatcg tgtagaactc
     6421 gatgccttca tgccggccca agacccgccc gtgcaggtcg acaatgtccc ctgggtgcgg
     6481 tgcgacgagc ctggcctcgc gcaggaaacg tccgtagtcg ttgtcgggca cgaagcagat
     6541 ttccatgctc tccgccttct ccacggcggc gacctggtgc gcacgcgcga tctcgcgcgt
     6601 ctgtgacttc agaagatcgc ccagcggcag caaggcgtgg gcgagctgtt cctggttcag
     6661 ggaaaacagg aagtagctct gatcgcgccg cgaatcccgt cctcgacgga gcagccaacg
     6721 gcccgaggcc tcgtttcgtt ccacgcgggc gtagtggccg aaggcaatct ggtgtgcccc
     6781 caatcgccgt gcgcaggaga gaagcaggtc gaacttgatc ctcgcgttgc acatcgcgca
     6841 cggattgggc gtgcgccccg ccctgtactc gtccgcgaag tattggatga ttcgctgccg
     6901 gaatgcgtct gcgccatcca cgacatgaaa tggaatcccc agcctgtcgc acaccgcacg
     6961 ggcatcctca gcagtcgctg cgtccgaacg ccgtgagaag cgctcctgcg ctcctggcat
     7021 cgcctcgggc gaccagagct tgagcgtgac gccgacgaca gcgtaaccct catttcggag
     7081 cagaatggcg gcagtcgagg agtcaacccc gccgctcatc ccgacaacca cccgagtgct
     7141 gagccggtcg gacacgcgcc cgagggtagg ggaggcgttc ggactgtgta aaggcccgga
     7201 cgtcatcgtg tcgacagccc ggagccggtc ttctgctgat agtagcgcca tcggcggatg
     7261 acggactcga catagtgtcg ggtgctggga aaggcaatct gcgacacgaa ctcgacgctg
     7321 ttcgtggcag cagcctctct cgcccacttg agcacattcg cgcgtcccgc attgtagtcc
     7381 gccagcgcgt aggggacggg atcgtccgtc ttccggtaac gctcgatcag cttgcacagg
     7441 taccaagtcc ccgcaaaagc gttggtgcgc gggtcataca gatgcgcctc gggcaacgga
     7501 taggcgcgga ccgtttccgc ccactcctga gcagccgcgt cggtcaactg catcaggccg
     7561 atctcgcccg ctcgcccccg cacgcccgga tggaatcggc tttccctcca gaccacagcc
     7621 ttcaccaggg ccggatcaag cttgtagcgc ctggctgcct cgaggatcgg cgcgtcctgg
     7681 ctgtgatcga gtcgttcgct ggcccttcgc cacaacgcca gcaatgcgat cagcagcagg
     7741 accagacaga ccgccgctcc gccaagagcg aacgttctgt ccctgtgcag cccggtcttc
     7801 acgcgtgacc acctagcccg catgctccct cattccttgc gtcgtgtgtc gatgataacg
     7861 gtgacggggc catcgttgac cagggccacc ttc
//
LOCUS       DAOQRO010000140         7862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8856_length_7862_cov_5.374408, whole genome shotgun sequence.
ACCESSION   DAOQRO010000140 DAOQRO010000000
VERSION     DAOQRO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8856_length_7862_cov_5.374408"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKK20_06730"
     CDS             <1..213
                     /locus_tag="PKK20_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99616.1"
                     /translation="VKANTPSPSDSTRPPTSKNYNLNCNLSALRICLIALKADLHPEA
                     SWPALQERCQNNPQIPFQILVKHRSK"
     gene            591..1022
                     /locus_tag="PKK20_06735"
     CDS             591..1022
                     /locus_tag="PKK20_06735"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNU99617.1"
                     /translation="MSSNVTLLAIFAENRTGQLAHVTRILADAVINIRWIIIASSDAF
                     GVVKLLVDNTDLAYRKLKEDGLSVSRVEVLAIEVDDKPGGLLEVADALSRSRINIENA
                     SGFVANGRAVLLIEVKELERARQALESVPLRLLSQAETLRL"
     gene            1387..4053
                     /locus_tag="PKK20_06740"
     CDS             1387..4053
                     /locus_tag="PKK20_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNU99618.1"
                     /translation="MKILTATFFVTVLGAALADQDAIPATVLSRFEFREPDFVVYRIE
                     TPARAADLGSVEDSVWLKAWPEAGAPNATWLGRRVVLQAASPEALARVLAATGLPVAR
                     VVTSNLVVLEASSARMAVAEASRLARHPGVTVCHPVRRRPIKLHGPYAPRPDDTLFRY
                     QWHLENRDANTAAVLGFDLNARSAWADNQGEGVLVATGDNGIEVSHPDLAANMTNRIH
                     RNFITGKDDGTPVSTTQVHGTAVAGLIAARGYNARGVAGVAPRAQLASWVIFDRYDGL
                     ADEETVMDMFHYQSNIVSVQNHSWGNADMGLLRLSALEDQGIANAVERGRQGRGVVIV
                     RSAGNGRMNLSDLNDDGFGQDPRAISVGAVRANGRAASYSTPGAPVLVAAFSGDEGVN
                     TPDGSVTNYLGVATTDRQGSLGYNSSGPAPDYGFQSTGFGGTSASSPQIAGLCALILA
                     ANPALTYRDVQQVLILSSRQLDPSDSDLRVNGAGLAVDHNVGFGVPDAGLAVELARAW
                     SNRPARIRTTAAFRKSIEIPDDGLRVVIGGSDVPTELQSIPAWPSDGPHPDAPTEVAT
                     LVDVGQALTPLAIDLTHKAALIKRGVNYFADKIGKAAAAGARFAIVYNNVGANQRVFM
                     AGADIQFQTIPAVFIDQTHGEALQAFLATHPETTAQLRLDSIQCPMKISESLLCEHVK
                     LRATFSHPRRADVRVTLLSPAGTRSVLHRFNNDTASALTEWSFYSTQHFYESSAGVWQ
                     VEVSDENAGSTGRVVSLDLTVYGVRIEDVDRDGLDDPWELAWFGSLDQGPAGDPDKDG
                     WSNLHEQILGHNPTVADRPFRLDLSHWGAQWGRLSWPASAAFRYEVSMQSALGEPAQV
                     VSEQTGRFPELDLIVPLTDTHHFYQVRQVPGD"
     gene            complement(4270..5691)
                     /locus_tag="PKK20_06745"
     CDS             complement(4270..5691)
                     /locus_tag="PKK20_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNU99619.1"
                     /translation="MKLWLLLVSAALAAPLPGIPADAELTVIPARKAYLLPIRDNIMP
                     PLVYLVRRGVKEAMDAKADLLVLDMETNGGRVDSTEEIIGILSQFKGETVTYVNRSAY
                     SAGAFIAVATQRIFMAPESVIGAAAPIMLSPGGTGVEAMPETMEIKATSAISAKIRAQ
                     AQKNGHNSDVVEAMIDKQKELIIDGQTINAKGGILTLTNVEAERAYGTPPRPLLSAGT
                     VESLDALLSRLGYEDARWHEIQPTGAEKLATWINALNWLWLIIGVAGIYIEFKTPGFG
                     LPGIVGVCAFALYFLGSYIAGLSGFEWPALFVLGLILVGLELFVFPGTVLLGLTGAAL
                     MLITIVMAMVDIYPGMPRLPSLPQLTLPLRDLTYAALGSVLVLAVLARLLPKTAVYGT
                     LVTQAASAMGSVAADAKVQAAQLGLEGVAVSVLRPGGKAQFGADLLDVMTQGELISKG
                     TRVRIIGHSGREAIVEAAVSPQD"
     gene            complement(6175..7662)
                     /gene="gltA"
                     /locus_tag="PKK20_06750"
     CDS             complement(6175..7662)
                     /gene="gltA"
                     /locus_tag="PKK20_06750"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNU99620.1"
                     /translation="MAHKTTSSLAAEARARLDALAGRTHLSTAQRREIPVQEMPHQPP
                     CERVRNMSEVALGYTPEQARLEALRCLECKNAPCIAGCPVGVNIPGFIGAIAHGDLQR
                     ALEVVKESNLLPAICGRVCPQETQCQARCVVGKMHKNPNRAVSIGRLERFVADFGRQQ
                     HWAAAPEIKPATGKKVGIVGSGPASLTAAADLRRAGHAVTIFEAFHKPGGVMIYGIPE
                     FRLPKRIVDAEVEALIRIGVEIRTNFVVGRTRTMAQLLDEDGYDAIFIGTGAGLPKFM
                     HVEGESLVGVYSANEYLTRVNLMRAYDAQHAGTPILRPRRVAVFGGGNVAMDSARSAL
                     RLGAEQVHLIYRRTEVEMPARLEEIQHAKEEGVQFHFLENAKRILGDEADRVIGVELL
                     RYRLGEPDSSGRASPVAIDGSEFTLDVDGVIVAIGNDSNPLLVQATPGLKADKYGRIQ
                     VDQDLRTSLDRVYAGGDIVQGAATVIIAMGQGRRAARSINALLAG"
     gene            complement(7652..>7862)
                     /locus_tag="PKK20_06755"
     CDS             complement(7652..>7862)
                     /locus_tag="PKK20_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNU99621.1"
                     /translation="TGMCGGCRVTVGGQVKFVCVDGPEFDGHQVDFESMAKRMQTYRQ
                     QEQSAQHRCRLAQHPPHNQEKPSGP"
BASE COUNT         1382 a         2569 c         2471 g         1440 t
ORIGIN      
        1 gtgaaggcga acacaccgtc gccctccgac tctacgcgac caccaacctc caaaaactac
       61 aacctcaact gcaacctctc ggccctgcgc atctgcctga tcgcgctcaa ggcggacctt
      121 caccccgagg cttcttggcc tgccctgcag gagcgctgcc agaacaatcc ccaaatccct
      181 ttccagatac tcgtcaagca ccgctccaaa tgaaaagacc gtgcggagga ggcggcgagg
      241 ccgtgcatcc ggatgttgtg ggtgggaatc gagggctctc ttcgcgctca cgaaagcgtt
      301 ggagtgggga cgccctcgtc cccggcgatg ggagttgcag accctgtttg acgggcgacg
      361 aggacctgcc tgccctgccg gcaggcgggc gtcgcccctc cgccaatctc catccgttgc
      421 tggcccggcg ccctcaccgg gtgacatcac accgacaaca actgaatccg ccgcgcagag
      481 gctgccgggc cgtgtggagc gcgttcgagg aatgaatcga gtggacgcgg agcggggacg
      541 cggggtaagc tgcgcgcgaa aagttcatac accccaaccg cgcattgttt atgagcagca
      601 atgtcactct gctggcgatc tttgccgaga atcggacggg ccaactggcg cacgtgaccc
      661 gcatactcgc cgatgctgtg atcaacatac ggtggatcat cattgcgagc agcgacgcct
      721 ttggggtcgt gaagctgttg gtggacaaca cggaccttgc ctatcgcaag ctcaaggagg
      781 atggcctttc cgtctcgcgc gtcgaggtgc ttgcgatcga agtcgacgac aagccaggcg
      841 ggctgctcga ggtcgccgac gccctctcgc gaagccggat caatatcgag aatgcgtccg
      901 gctttgtcgc gaacggccgc gccgtgcttc tgattgaggt caaggaactc gaacgggcgc
      961 gtcaggcgct tgagtctgtt ccgttgcgcc tgctgagcca ggcggaaacg ctgcggctct
     1021 aggggagtct cgcgtcgaaa aggcgcctcg ccggtcgcca tttcgaacag catcactccc
     1081 aggaaaaaca aatcgctgcg tgcgcccacg gcatcggccc tgatctgctc cggactcatg
     1141 taggcgggcg ttcccttgat cgcgccgatc tcgggttgag tcatcgtgga tacgccgacc
     1201 ccggccaccg caggggtcag ggtcgattcg gtcggatcgg cgagtttggc caggccgaag
     1261 tcgagaatct tcgcgtgggc gtcccgtgtg accatgacgt tggcgggttt gacatcgcga
     1321 tgcatctctc cggcctatct tccgctggct tccctggcgg cccgtggtac cgtcggcgac
     1381 gatcttgtga agattctcac tgctaccttc ttcgtgacag tcctgggggc ggcgctcgcg
     1441 gatcaggatg cgatccctgc gacagtgctg tcgcgcttcg agttccgcga accggatttc
     1501 gtcgtctatc ggatcgagac acccgcccgc gccgcggatt tgggctctgt cgaagattcc
     1561 gtctggctca aagcatggcc ggaagcgggg gcgccgaacg ctacgtggct tggccgccgg
     1621 gttgtcttgc aggccgcatc gccggaggct ctggcccggg tccttgcggc gactggactg
     1681 cctgtcgccc gtgttgtgac gtcgaatctg gttgtgctcg aggcctccag cgctcgaatg
     1741 gcggtggcgg aagcatcgcg tttggcgcgc catcccgggg tgaccgtctg tcatccggtc
     1801 cggcgtcgtc cgatcaaact ccacgggcct tacgccccgc gacccgacga cacgttgttt
     1861 cgttatcaat ggcacctcga gaaccgggat gccaacaccg ctgccgttct cggcttcgat
     1921 ctgaatgccc ggtcggcatg ggcggacaac cagggcgagg gtgtcttggt ggccacaggc
     1981 gacaacggca tcgaggtatc gcatccggat ctggccgcca acatgacgaa ccggattcac
     2041 cgcaacttca ttactggcaa ggacgacgga actcccgtgt ccaccacgca ggtgcatggc
     2101 accgcggtgg ccggcctgat cgccgcccgg ggctacaatg cccggggtgt tgcgggagtc
     2161 gcgcctcggg cccaattggc cagttgggtc atcttcgacc ggtacgacgg tttggcggac
     2221 gaggaaacgg tgatggacat gtttcactac caatccaaca tcgtcagcgt ccagaaccac
     2281 agttggggca atgccgatat ggggctgctg cgcctgagcg cactcgagga ccaggggatc
     2341 gccaacgcgg tcgaacgcgg tcgccagggg cgcggtgtcg tgattgtccg gtccgcgggc
     2401 aatggccgca tgaacctgag cgacctcaac gacgatggct tcggccagga cccgcgggcg
     2461 atttcggtcg gcgccgtccg ggcgaatgga cgcgccgcga gctacagcac gccgggcgcg
     2521 cccgtgctcg tcgccgcatt cagcggcgat gagggcgtca atacccccga cggatcggtc
     2581 acgaactatc tgggagtggc tacgacggat cgccagggga gcctcggcta caactcctcc
     2641 ggcccggctc cggactatgg attccagtcc acagggttcg gggggacctc ggcgtcgtcg
     2701 ccgcagatcg ccggcctctg cgcgctgatt ctggccgcca accccgctct gacctatcgc
     2761 gacgtccagc aggtcctgat cctgtcgtcg cgccaactcg atccctcgga ttccgatctg
     2821 cgcgtgaacg gcgcgggatt ggcggtggat cacaacgtcg gttttggcgt tccggatgcc
     2881 ggtctggcgg tcgagctggc gcgcgcctgg agcaatcggc cggcgcgaat ccgaacgacc
     2941 gcggcctttc gcaagagcat cgaaattccc gatgacggac tacgcgtcgt gatcggaggc
     3001 agcgatgtgc cgaccgagtt gcagtcgatt ccggcctggc cctccgacgg tccgcacccg
     3061 gatgcgccca ccgaggtggc gacgctcgtg gatgttggcc aggcgctcac ccctctggcg
     3121 attgacttga cgcacaaagc ggcgctgatc aagcgagggg tcaactactt cgccgacaag
     3181 atcggcaagg cggcggcggc gggcgcccgg tttgccatcg tctacaacaa cgtcggcgcc
     3241 aatcaacggg tgttcatggc tggggccgac attcagttcc agaccatccc ggcggtgttc
     3301 atcgaccaga cacacggcga agcccttcag gcgttcctgg caactcaccc ggagaccacc
     3361 gcgcagctcc ggctcgactc gattcagtgc cccatgaaaa tctcggagtc gctgctctgc
     3421 gaacacgtca aactgcgtgc caccttttcc cacccgcgcc gggccgacgt gcgcgtgacg
     3481 ttgctctcac ccgccggcac gcgcagcgtg cttcaccgtt tcaacaacga cacagcttcg
     3541 gctctgactg aatggagctt ttactcgacc cagcacttct acgagagcag cgccggtgtc
     3601 tggcaggtcg aggtcagcga cgaaaatgcc ggctcgacgg gacgcgtcgt ctcgctggat
     3661 ctgacggtct acggggtgcg gatcgaggat gtggatcgcg acggattgga cgatccgtgg
     3721 gaattggcct ggttcggctc gctggatcaa gggcccgccg gcgaccccga taaggacggt
     3781 tggagcaatc ttcacgagca gatcctgggg cacaacccga cagtcgcgga tcgaccgttc
     3841 cggctcgatc tgtcgcattg gggcgcccag tgggggcgac tcagttggcc tgccagcgcg
     3901 gcattccggt acgaggtgtc gatgcagtcc gccctggggg agccggctca ggttgtgtcg
     3961 gagcaaaccg ggcggtttcc cgaactcgat ctcattgtgc ccctgaccga cacacaccat
     4021 ttctaccagg tgcgccaagt gccgggcgat tagcgaggct gcgcctcaga cctgcgagta
     4081 cggtttcttg gcactgatcc cgctggcttt gctcatgtgg ggattgattg gcattgtccg
     4141 agcggccgcc cgcggttgac tcgcgctctg ccttcgccgt tcatcatcgc ttcgtttttc
     4201 cgcttgggcg cacacgtccg actacgtcag gggagtgtcc gccaaggcgc cggtctgagg
     4261 cgcgcctcgc taatcttggg gagaaaccgc ggcctcgacg attgcctcgc gtccactgtg
     4321 gccgatgatt cggacgcgtg tgcctttgga aatcagttcg ccctgggtca tcacatcaag
     4381 gaggtccgcg ccgaactgag ctttgcctcc ggggcgcagc accgagactg ccaccccctc
     4441 gagccccagt tgcgccgcct gcactttggc gtcggctgcc acggacccca tcgcgctggc
     4501 cgcctgggtc acgagcgttc catagaccgc cgtcttgggc agcaaacggg ccagcactgc
     4561 caataccaga accgatccga gggccgcgta ggtcaggtcg cgcagcggca gagtcagttg
     4621 tggcaggctg ggaaggcgcg gcattccggg atagatgtcg accatggcca tgacgatcgt
     4681 gatgagcatc agcgcggctc ccgtgagtcc cagcaggacc gtgcccggaa acacgaacaa
     4741 ttcgagcccc accaggatga ggcccagcac aaacagcgcg ggccactcga accctgacag
     4801 tccggcaatg tagcttccca gaaagtagag cgcgaaagcg cagacaccca cgatgccagg
     4861 taggccgaat ccgggcgtct tgaactcgat gtaaatgccc gcgactccaa tgatgagcca
     4921 aagccagttc aacgcgttga tccaggtcgc cagtttctcc gccccggtcg gttggatttc
     4981 atgccatcgt gcatcttcat agcccagcct cgacagcagg gcatcgagac tttcgactgt
     5041 gccggcagac agcagcgggc gtggcggggt cccgtaagcc cgttccgcct cgacgttggt
     5101 gagggtcaga atgccgcctt ttgcattgat ggtctggccg tcgatgatca gttccttttg
     5161 cttgtcgatc atcgcctcga ccacgtcgct gttgtgtccg ttcttctggg cctgcgcccg
     5221 aatcttggcg ctgatggccg atgtcgcctt gatctccatc gtctccggca tggcctcgac
     5281 tcccgttcct ccgggcgaga gcatgatggg cgcggcggcg ccaatcacac tctcgggcgc
     5341 catgaaaatg cgctgggttg cgaccgcaat gaacgcgccc gcggaatacg cactgcggtt
     5401 gacataggtc acggtctcgc ctttgaactg cgagaggatt ccaatgatct cttcggtcga
     5461 gtcaacacgc ccgccattgg tctccatgtc cagcaccagc aggtcggctt tggcatccat
     5521 cgcctccttc accccccgcc gcaccaggta caccagcggc ggcatgatgt tgtcgcgaat
     5581 cgggaggaga taagccttcc tggccggtat cacggtaagc tcggcatcgg ccgggatgcc
     5641 gggcagcggc gcggccagcg cggcggaaac caaaaggagc cagagcttca tgaccggaca
     5701 ctaacgcact ccggctcggc ctgcaagatg gccagccgca cggcctgttg ccgcctgacg
     5761 aaagtgcgtg aatcagaacc tgaacgccca tgttgagccc cgccaggccg cctgcaatcg
     5821 tcaccctcaa accgcgccag agcagggagg tcgaggcgcc gcacaacggc gacgccggga
     5881 ctcggactgg gccacgtctg aaccagtgac agaaggcacg ccagggcagg aagcagcgtc
     5941 agggctgcca ccagagctga agcgcgcagc agcgcttcct ggtctccctg agatcgtgtg
     6001 ctgctccaca atccgcccgc ggcggccagg aaaacagtga cgggcaacac gagttgcaga
     6061 cgccagaatc cccctgcggt cacaccgccc gcgcctattg ccaggctcag gatggggaac
     6121 gcagccatga gaaagggcaa tcacagggct tcgaccgcca ctcgggcgag ccgctcaccc
     6181 tgccagcagg gcattgatag atcgggcggc ccggcggccc tggcccatgg cgatgatcac
     6241 ggttgccgcg ccttgaacga tatccccccc ggcgtagacg cgatccaggg aggttcgcag
     6301 gtcttgatcc acttgaatgc gcccgtactt gtccgccttg agacctgggg tcgcctggac
     6361 cagcagggga ttcgagtcgt tgccgatcgc cacgatgacg ccgtccacgt ccagagtgaa
     6421 ctcgctgcca tcaatcgcca ccggcgaggc gcgtccggac gagtcaggct cgccaagccg
     6481 atatctcaac aactcgacgc cgatcacccg gtccgcttcg tcgcccagaa tgcgtttcgc
     6541 gttttcgagg aagtgaaact ggacgccctc ttccttggca tgttgaatct cctcgaggcg
     6601 cgccggcatc tcaacctcag ttcggcggta gatcaaatgc acctgttcgg cgcccagccg
     6661 aagagcgctt cgggcggaat ccatggcgac gttgccgccg ccgaacacgg ccaccctgcg
     6721 cgggcgcagg atgggcgtgc cggcatgttg ggcatcatag gcgcgcatca gattcacgcg
     6781 cgtgagatac tcgttggcgc tgtaaactcc cacgagactc tccccctcta cgtgcataaa
     6841 cttcggcagg ccggctcccg tcccgatgaa gatcgcatcg tagccatcct catcgagcaa
     6901 ctgcgccatc gtgcgagtgc ggcccaccac gaaattggtt ctgatctcga cgccaatgcg
     6961 aatcagggcc tcgacctccg catcgacgat gcgcttgggc agccggaact cggggatgcc
     7021 gtagatcatc actccgccgg gtttatggaa tgcctcgaag atggtcacgg cgtgccccgc
     7081 gcgccggagg tcggcggccg cggtcaggct ggctggaccg ctgccgacaa tcccgacctt
     7141 cttgccggtg gcaggtttga tctcgggcgc ggctgcccaa tgctgttggc gtccgaaatc
     7201 cgcaacgaac cgctccagcc gcccgatcga taccgcccga ttcggattct tgtgcatctt
     7261 tccgacgacg caccgcgcct ggcactgggt ctcctgagga cagacgcgcc cgcagatcgc
     7321 cggaagcaga ttgctctcct tcacaacttc gagcgcccgc tggaggtctc cgtgcgcaat
     7381 cgctccgatg aaccccggga tattgacgcc gaccgggcag cctgcgatgc agggggcgtt
     7441 cttgcattcg agacatcgca gagcctccag tcgggcctgc tccggcgtgt agcccagcgc
     7501 cacctccgac atgttccgaa cccgctcgca cggaggctgg tgaggcatct cctggaccgg
     7561 gatctcgcgg cgttgggccg tggagagatg cgtccgtccc gccaatgcat cgaggcgcgc
     7621 gcgcgcttcg gccgccaggc tactggtcgt cttatgggcc actcggcttc tcctggttgt
     7681 gcgggggatg ctgggccagg cgacaccgat gctgggcgct ctgttcctgc tgcctgtatg
     7741 tctgcatccg tttcgccatg ctctcgaagt cgacctgatg accgtcgaac tcaggtccgt
     7801 cgacgcacac gaacttcact tgaccgccca ccgtgacgcg gcacccgccg cacatgccgg
     7861 tg
//
LOCUS       DAOQRO010000141         7860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8861_length_7860_cov_4.704548, whole genome shotgun sequence.
ACCESSION   DAOQRO010000141 DAOQRO010000000
VERSION     DAOQRO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8861_length_7860_cov_4.704548"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKK20_06760"
     CDS             complement(<1..474)
                     /locus_tag="PKK20_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNU99622.1"
                     /translation="MSLPVQLISTDFDGTIFAEFENPPVPETLQRLLQDLQARGARWV
                     INTGRDLTSLMEAMARSGLGVWPDYLVLVEREIYIHQDHRYVGLTDWNDRCARDHAAL
                     YKRMRADVPRLTAWINEHFNAEVYADPWSPLCLLARSNPDTDAIEAFLREYCRTVP"
     gene            560..838
                     /locus_tag="PKK20_06765"
     CDS             560..838
                     /locus_tag="PKK20_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99623.1"
                     /translation="MKSAYELAMERLNRDSPIKKLSDAQKTKLAELDSIYAAKIAERE
                     IALTNDIRAAEIKGDIEAAEQARRILAAERAKLQAELETQKDRIRAQD"
     gene            complement(850..2670)
                     /locus_tag="PKK20_06770"
     CDS             complement(850..2670)
                     /locus_tag="PKK20_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HNU99624.1"
                     /translation="MAFGRLTWERLAAAGQAYLARRWQRLLALRQHLRLSEDAIHIAL
                     AVAIGLVAGLTNFVFFGATELIKWLTLNRLGDLTEIAEGLGPWTRLAGPALGGLVAGL
                     ALHWGLRVVGTQGSTNLLEVVVAGDGRLPFRTTGIKSLSSLISIATGASIGREGSLIQ
                     LSATFASQGGQRLRWPPYRLRLMVACGAAAGMAAAYNAPIAGAVFAAQIVLGNFAMNL
                     FAPLVCASVSAAVVSRSFFGIEPWYTVPDFNFTSLTQLPWFVVLGAASGTLGALFLKM
                     LRWGETLFDRTGWPIHIRLAAAGLVVGSLAIQVPEVWGNGYVVTSRILGDEGIYSTLF
                     VLALLGAKLVATALTVGAGTVGGVFTPTLFLGAAMGSLFGHGLHALGMADALPAGAFA
                     LVGMGSVLAATTRSPLLAMIIVFEISLNYSLMPALMLACAVGTLVGRSLHPASVYTEP
                     LQRKGLFVETESARLGAAIERTVGDLMLAPVAPVRETTPLQEIGKRFLTIPNNYLPVV
                     DARQRLVGVVSLHDLKGHLNAGEELRAVIALDIMRPPPPCLTPNQRLQDALPVLLSSE
                     MRNVPVVNNLFEFRLTGALSRGEALRLFTDAIAARQAARD"
     gene            complement(2689..2850)
                     /locus_tag="PKK20_06775"
     CDS             complement(2689..2850)
                     /locus_tag="PKK20_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99625.1"
                     /translation="MSASGRRLGWLGLLLALATWLGAGCASDSGSKEFIPGKGWRSTA
                     VESGPARGG"
     gene            3005..3565
                     /locus_tag="PKK20_06780"
     CDS             3005..3565
                     /locus_tag="PKK20_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNU99626.1"
                     /translation="MPKAPKFLEGQLLLDGGNLYGSWFHRTVILICQHDAEGAFGLVL
                     NRLSGNKVGDALVADLPDRIKELPLFVGGPVQGTAMSFLHTDSYLPNANVLPNLRLSH
                     SLDELVELGDSFSSTQQFRLFAGYAGWSPGQLENEMRRNAWLVHPATVDLVFHTEPAR
                     LWQHILRKKGWRYRLLAESPEDLSWN"
     gene            complement(3724..4683)
                     /locus_tag="PKK20_06785"
     CDS             complement(3724..4683)
                     /locus_tag="PKK20_06785"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNU99627.1"
                     /translation="MMLRRHGVLAAVVLVLLAAWTTIADGGTVVFETTSVYHHIRVVE
                     AGGYRSLLFDSSEQSRIALADPEAGRFEYIDYFFMPWLWNGAMTNVLMIGLGGGSAQQ
                     AYARYCPGVTVETVEIDPVVLKVAEDYFGFRQSPRQRVVISDGRVFLRRSRRQYDAVL
                     VDAYVEGRYGASIPYHLATQEFFGLVNDRLAPNGVVAYNCIGVLQNNKADLVGAIYRT
                     MKSVFPQVYLFPARESWNVVLVGTHSAEAKRAHELWPNAMPAWQSGRMRLPTFFQRLG
                     VLRVAPPSSSINSPLLADDYAPVDGLLGGMRPLRLFHTLREEP"
     gene            5143..5430
                     /locus_tag="PKK20_06790"
     CDS             5143..5430
                     /locus_tag="PKK20_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNU99628.1"
                     /translation="MLWPGENYGFQIVQALKRIEDLSVSESTVYPILGRLRDEGYLRS
                     RDARSDVGPPRRYFALTPPGKARLAEMNAYWLMLTQASERWKSDHEKEAES"
     gene            5427..>7860
                     /locus_tag="PKK20_06795"
     CDS             5427..>7860
                     /locus_tag="PKK20_06795"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNU99629.1"
                     /translation="MKWNEASARSAEAYLGAVERHLAHRTAAVRRAVLSSLRDHIAEA
                     MRRAEAEGETSLDAIERILAEMDPPESFAEEASSGASATASPTSSCRGSGRWFALGLA
                     FLLVNAYGVWKWTAQLASRGEPAAGPAPEPVRLEKTAKPADEVLRLRKVEQADVSPDR
                     AVTLVFEFNETPVREQLTRHLHLSAEGQGDVEYRLAGAMGPNTVMVETQPVLAEKLEY
                     RLAPGLPSASDARPLDREERGVLTMEVKMLLRGIDAECPSFEPPSLVLKFTAFPEASQ
                     AKEFILIEPAAAYTIEAVESWQGKRFLLRGDFSPGGIYTVTLRSGLPAANGSSLPETV
                     KRVVQFPMPSPAVRLDEPGRYLAPGGALSVPVRAVNLKEMVVALMPVFENNLVELARR
                     ESDYHLGALTTDLAGPRRAATNSLPASTGGATVRAAVDLRALAGAEPRGVYWIEVGGP
                     QANGDGRLLVVTDLGIVARTFRGGVLVWVNSLRKAEPAAGVAVQVLARNNQALARGTT
                     DAQGMARFEWPADDGTEPFVITAALDGDLSYLDLAGTKVDQGEGLAGARYLAPGEVEA
                     AVFTDRGVYRPGETMFLQAIARDSQSRAPEPFPALVRVRRPDGRVFQDVPVGLDEYGC
                     ARTEVRLPEFLPTGRYLVELALPGTFTVLGETSVALEDFVPPHIRVDVQAPNEPAEAG
                     DTLNFGVRAVYLFGRAAHGLRVTGTATINPAPFASPNWPGWWFGDSEKEFAPVHQKLG
                     AGWLDAQGRCGFDVESLRAWRPPASLRVVLGATVMEPNGRAVTAYGATRLDVYPFYVG
                     LKQAWEGAVRTGE"
BASE COUNT         1483 a         2477 c         2587 g         1313 t
ORIGIN      
        1 cggcacggtg cggcagtact cgcgcagaaa agcctcgatg gcgtcggtgt ccggattgga
       61 ccgagccagc aggcagagag gggaccaggg gtcggcgtag acctcggcgt tgaagtgctc
      121 gttgatccag gccgtcaggc gtggcacatc ggcgcgcatt cgcttgtaga gggccgcatg
      181 atcgcgcgca cagcggtcgt tccagtccgt caggccgaca tagcgatgat cctgatggat
      241 gtagatctca cgttccacga gcaccaggta gtccggccaa acgccaaggc cggaccgcgc
      301 catggcctcc atcaggctgg tcaggtcgcg tccggtgttg ataacccagc gggctccccg
      361 agcctggagg tcctggagca gtcgctgcag ggtttcgggc accggcgggt tctcgaattc
      421 ggcgaagatt gtgccatcga aatcggttga tatgagctga acgggcaatg acatgatttg
      481 agtcgaggtc caaaaggttc ggttgggccc ttgcgcagcg ggcagaatcg tgtcatatgc
      541 gcctttgtca aaacgattta tgaagtcagc ttacgaactg gccatggagc ggctcaatcg
      601 agactcgccg atcaagaagc tctcggacgc ccagaagacg aaactcgccg aactcgattc
      661 gatctacgcg gccaagattg ccgagcgtga gattgcgctc acaaacgaca tacgcgccgc
      721 cgaaatcaaa ggggacatcg aagcagccga gcaggcccgg cgcatccttg cggccgagcg
      781 cgcaaagctc caggccgaac tcgagaccca aaaagaccga attcgggccc aagactgacg
      841 gcgtgcggat caatcgcgcg ccgcctgccg ggcggcgatg gcgtcggtga aaagcctgag
      901 cgcttcgccg cgcgagagtg ctccggtgag tcggaattcg aacaggttgt tcactacggg
      961 gacattgcgc atttcgctcg aaagaaggac ggggagggcg tcctggagcc tctgattggg
     1021 cgtcaggcac gggggaggcg ggcgcatgat gtccagggcg atcacggccc ggagctcctc
     1081 tccggcgttc agatgcccct tcagatcgtg cagagacacc actcctacca ggcgctggcg
     1141 agcatccaca accggcaggt agttgttcgg gatcgtgaga aaacgcttgc caatctcctg
     1201 gagcggcgtg gtttcgcgca ccggcgcgac cggggccagc atgaggtcgc cgacggttcg
     1261 ttcaatggcc gcgccgaggc gcgcgctctc ggtctcgaca aacagtcctt tgcgctgcag
     1321 cggttcggtg tagacggatg ccgggtgcag gctgcgtccc accagcgtgc cgaccgcgca
     1381 cgcgagcatc aaagccggca tcagtgagta attcagcgag atctcgaata cgatgatcat
     1441 ggcgagcagg ggcgagcgtg tggtcgcggc cagaacactg cccatgccga cgagcgcaaa
     1501 cgccccggcc ggaagggcgt ccgccatacc caaggcgtgc agcccgtggc caaagaggct
     1561 ccccatcgcc gcgccgagaa acaaagtggg cgtgaacacg ccgcccaccg tccccgcgcc
     1621 tacggtcaaa gcggtggcaa cgagcttggc gccgagcaag gccagcacaa acaacgtgct
     1681 gtagatgccc tcgtcgccga ggatgcgcga ggtgacgacg tagccgttgc cccacacctc
     1741 cggcacctgg attgccaggg acccgaccac aaggccggcg gccgccagcc ggatatggat
     1801 cggccatccg gtccggtcga acagagtttc tccccacctc agcattttca aaaacagggc
     1861 gccgagggtt cccgaggccg ccccgagcac tacaaaccac ggcagttgcg tcagactggt
     1921 gaagttgaag tcgggcaccg tgtaccaggg ttcgatgccg aaaaagctgc gcgacacaac
     1981 cgccgccgag actgaggcgc acaccagagg tgcgaacagg ttcatcgcga aattgccgag
     2041 cacgatctgg gcggcaaaga cggcgcccgc aatcggggcg ttgtaggccg cggccattcc
     2101 tgccgcggca ccgcaggcca ccatcagccg caagcgatat ggtggccagc gcaagcgctg
     2161 acccccctgc gacgcgaagg tcgccgaaag ctggatgagc gatccttcgc gtccgatcga
     2221 tgcgccggtg gcgatgctga tcaaggacga gagcgatttg atgccggttg tgcgaaaggg
     2281 caatcggcca tcgccggcca ccaccacctc gagcaagttg gtcgagccct gtgtgcctac
     2341 cacgcgcagg ccccaatgca gcgccagccc cgccaccaga ccccccagcg caggccccgc
     2401 cagccgcgtc cagggcccca gtccctccgc gatctccgtc agatcaccca gccggttcag
     2461 agtcagccat ttgataagtt ccgtcgcacc gaagaagacg aagttagtca gtcccgccac
     2521 caggccgatc gcgaccgcga gcgcgatgtg gatggcgtct tcgctgagac gcaggtgctg
     2581 tcgcagggcg agcaaccgct gccaacggcg cgccaaataa gcctggccgg ccgccgcgag
     2641 tcgctcccac gtcaggcgcc cgaacgccat gaagtcacgc tcccgagatc aaccgccccg
     2701 tgccggtccg gattcgaccg ccgttgatct ccagcctttg ccgggaatga actccttgct
     2761 gcccgaatcg ctggcacacc ccgcgccgag ccaggttgcc aacgccagga gcaatcccag
     2821 ccatccaagt cttcgtccgg atgcactcat gggcgtcaat ctaatcgagg ttgtcagact
     2881 gacaagcttg ttgcacagtc tcgttgcgcc gcaaatgaga gccgggcggc ttgcgggcat
     2941 cggcacgccg cctcttccgg ctgggccgcg gcgggaccgc aggcggtcga ctgaagatcg
     3001 gatcatgcca aaggcaccca aattcctgga aggccaattg ctgttggacg gtggcaacct
     3061 ttatggatcg tggtttcatc gcacggtcat cctgatttgc cagcacgacg ccgagggcgc
     3121 attcggtctg gtcctcaaca gactcagcgg caacaaggtg ggggacgcgc tcgtggccga
     3181 cctgcccgat cggatcaagg aactcccgct ctttgtcggc ggccctgttc aggggaccgc
     3241 catgagcttt ctgcacaccg attcctacct ccccaatgcc aacgtcctcc ccaatctcag
     3301 attgagccac tcgctcgacg agcttgtcga gttgggcgac tccttttcat ccactcaaca
     3361 gttcaggctc ttcgccggct acgccggctg gagcccgggc cagctcgaga atgaaatgcg
     3421 tcgcaacgcc tggctggttc acccggccac agtcgatctg gtttttcaca cggaaccagc
     3481 gaggctctgg caacatatcc tgagaaagaa aggatggcgc tatcgcctcc tggcggagtc
     3541 ccccgaggac ctctcgtgga actagcgggc accagaccaa atccagttct cgtctgcatg
     3601 gggagtcccc gtgcttcccg acacctctct cccgcgcctc gctcagtagc atccttgccc
     3661 cgtttagcgc agcacagaaa ctcgacttgt ctacagcgga tttcaccggt caaggctagc
     3721 cggttacggt tcctcgcgaa gcgtgtgaaa taaccgtaag ggccgcatgc ctcccaacag
     3781 cccgtccacg ggcgcgtagt cgtccgccag caaaggcgag ttgatggagc tcgaaggcgg
     3841 cgcgacgcgc aagacgccca gccgctgaaa gaaggtcggg agccgcattc ggcccgattg
     3901 ccaggccggc atggcgttgg gccaaagctc gtgagccctc ttcgcctcgg cactgtgggt
     3961 ccccaccagc acgacattcc aactctcgcg cgcggggaag aggtagacct ggggaaacac
     4021 cgatttcatt gtccgataga tcgccccgac caaatccgcc ttgttgttct gcaacacgcc
     4081 gatgcagttg taggcgacga cgccgttggg agcgagccgg tcgttgacaa gcccgaagaa
     4141 ctcctgcgtg gcgagatgat atgggatcga ggcgccatac cgcccttcga cataggcgtc
     4201 gacgagcact gcgtcgtact gtcgccggct gcgccgcaga aacaccctgc cgtccgaaat
     4261 aacgactcgc tgccgcggcg attggcggaa gccgaaatag tcctccgcca ccttcaatac
     4321 gacggggtcg atctcgaccg tctcgaccgt cactccgggg caataacgcg cgtaagcctg
     4381 ctgcgcgctg ccgccaccca ggccgatcat caggacgttc gtcatcgccc cgttccaaag
     4441 ccacggcatg aagaagtaat cgatgtactc gaaacggcct gcttccgggt cggccagtgc
     4501 aatccgcgac tgctcggagc tgtcgaacag gagggagcga tatccccccg cctcgaccac
     4561 gcgaatatgg tggtagaccg atgtggtttc gaacacaacc gttccgccgt cggcgatggt
     4621 tgtccaggct gcgagtaaca ccaggacaac agccgccaac acgccgtgtc ttcgcagcat
     4681 catgagcaac tcgtggaaac gccggcagca gccggcaatg ttccagcccg cattggcaca
     4741 cctttggagg cgttcggctt cgccgcggtc acaagttcat ggcacatgcg ggccaggttt
     4801 cgacccgccc ttgatccgcg gcgatcccca cacaggcaac aaatggcgag cactgcgatc
     4861 aacgctccca tgagcctgaa aatgctcgat atccgcatca cgtccagcag aacataccct
     4921 gtggcgaaca cgccggcaat tccgcccagc gtgttggacg cgacaatcga cgcgctcgag
     4981 cgtccgagcc gatccatact gacggagacc catgcggccc cgctgatact tgacgggccg
     5041 ttctgtattc tgtattgggt aatatgtgat acggagtagc tcgcgatcga gggctggatc
     5101 atccagtagc gcaaggggct gatcgagtac tgcctgctgc tcgtgctgtg gccgggggag
     5161 aactacgggt ttcaaatcgt gcaggcgctc aaacgcatcg aggatctctc ggtctcggag
     5221 agcacggtct atcccatcct gggccggctg cgcgacgagg gatatctcag gtcgcgggac
     5281 gcgcgttcgg atgtcgggcc gccgcgccgc tattttgcgc tcacgccgcc gggcaaggcg
     5341 cgcctggcgg agatgaacgc atactggctc atgctgacgc aggcttccga gcgatggaaa
     5401 tccgaccatg agaaagaagc cgaatcatga agtggaacga ggcgtccgca cggagcgccg
     5461 aggcgtatct cggcgccgtt gagaggcatc tggcgcatcg gaccgcggcg gtgcgccggg
     5521 ctgtcctgtc gagtcttcgg gaccacatcg ccgaagcgat gcggcgcgcg gaggcggaag
     5581 gtgaaacgag cctcgacgcc atcgagcgga ttctggccga gatggatccg ccggaatcgt
     5641 tcgcggaaga agcgtcatcc ggcgcgtcgg ccaccgcttc tccgacgtcc tcttgccgtg
     5701 ggtcgggacg ctggtttgcg ctgggactgg cgttcctgtt ggtgaacgcc tacggggtgt
     5761 ggaagtggac cgcccagttg gcgtcgcgtg gtgagccagc ggcggggccg gcccccgagc
     5821 cggttcgcct cgagaaaacc gccaagccgg cagacgaggt tctgcggctc cggaaggtgg
     5881 agcaggcgga cgtgtcgccg gatcgggcgg tgacgttggt attcgagttc aacgagacgc
     5941 ccgtacggga gcagttgaca cgccatctgc atctttcagc ggagggacag ggcgatgtcg
     6001 agtatcggct ggcaggggca atggggccga acacggtgat ggtcgagacg cagccggtgc
     6061 tggcggagaa actcgaatac cgtcttgcgc cgggtttgcc ctcggcatcg gacgcgagac
     6121 cattggaccg cgaggagcgc ggggttttga cgatggaagt gaagatgctg cttcgcggca
     6181 tcgatgcgga atgcccctcg ttcgaaccgc cgtcgctcgt cctgaagttc accgcgtttc
     6241 cggaggcgag ccaggcgaag gaattcatcc tgatcgagcc ggcggcggcc tacacaatcg
     6301 aggcggtcga gtcctggcag ggcaaacgat tcctgttgcg aggcgatttc agcccaggcg
     6361 gcatctacac tgtcactctt cggtcggggc tgccggcggc caatgggtcg agcctgccgg
     6421 agacggtcaa gcgggtggtt cagtttccca tgccttcacc ggcggtgcga ctggacgaac
     6481 cagggcggta cctggcgccg ggcggcgcgc tgagcgtgcc ggttcgggcc gtcaacctga
     6541 aagagatggt tgtggcgctg atgccggtgt tcgaaaacaa cctggtcgaa ctggcccgcc
     6601 gcgagtcgga ctaccacttg ggcgccctga cgacggactt ggcgggcccc cgccgggccg
     6661 ctaccaactc gttgccggcc tcgacgggcg gcgcgacggt gcgtgcggcg gtcgacctgc
     6721 gtgcactggc gggcgcggag ccgcgcggcg tctactggat cgaggtcggc ggtccgcagg
     6781 ccaatggcga tggccggctt ctggtcgtca cggatttggg tatcgtggct cggacattcc
     6841 gaggcggggt gctggtgtgg gtaaactcgc tgcggaaggc ggagcccgcg gcgggggtcg
     6901 ccgtgcaggt tctggcgcgg aacaatcagg cgctggcccg cggcacgacc gacgcacagg
     6961 ggatggcgcg gttcgagtgg ccggcggacg acggcacgga gccgttcgtg atcacggcgg
     7021 cgctcgatgg cgacctgtcc tatctcgatc tggccgggac gaaggtcgat cagggcgagg
     7081 gcctcgcagg agcgcgttac ctggcgccag gcgaggtgga agcggccgta ttcaccgatc
     7141 gaggcgtgta ccgtccgggc gagacgatgt tcctccaagc cattgcccgc gactcgcaga
     7201 gccgcgcacc ggagccgttc cccgcgctgg tgcgcgtccg gagacccgac gggcgcgtct
     7261 tccaggacgt tccggtgggg ctggatgagt acggatgcgc gcggacagag gtcaggctgc
     7321 ccgagttcct gccgacgggc cgttacctcg tggaactggc cctgccgggc acattcacgg
     7381 tgctgggcga aacctcggtg gcactcgagg atttcgtgcc gccacacatc cgagtcgacg
     7441 tccaagcgcc caacgaaccg gccgaggcgg gtgacacgct caacttcggc gtgcgcgccg
     7501 tctacttgtt cggacgcgcg gctcatggat tgcgggtgac cggaaccgct accatcaacc
     7561 cggcgccctt cgcgtcgccc aactggcccg gatggtggtt tggcgacagc gagaaggagt
     7621 ttgcccctgt ccaccaaaag ctcggggcgg gttggctgga tgcccagggc cgttgcgggt
     7681 tcgacgtgga gagcctgcgg gcctggcgac cgccggcaag ccttcgcgtg gtgctggggg
     7741 cgaccgtgat ggaaccgaac ggacgggccg tgacggccta cggcgcaacg cgactggatg
     7801 tctacccctt ctacgtcggg ctcaaacagg cctgggaggg tgcggttcgc acaggcgaga
//
LOCUS       DAOQRO010000142         7834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8897_length_7834_cov_6.460728, whole genome shotgun sequence.
ACCESSION   DAOQRO010000142 DAOQRO010000000
VERSION     DAOQRO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_7834_cov_6.460728"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..827)
                     /locus_tag="PKK20_06800"
     CDS             complement(78..827)
                     /locus_tag="PKK20_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNU99630.1"
                     /translation="MALVFPSRLLTLAGMELSQGLRKVQERVAQACRRAGRQASEVTL
                     VAVTKGQPPEVVRAAAGLGLDIFGENKVQEAKAKIPLCLARLRWHMIGHLQTNKARDA
                     VHFFEMIQSIDSLRLAEEVNRCAEKAGRTMPILLEVNVAGEASKFGYRPETLLAEFDR
                     LNGLRRLEIHGLMTMAPWTPDPEKVRPVFRRLRELKAECEARLGAPLEHLSMGMSSDF
                     EVAIEEGATIVRVGTAIFGERHRSAPAASGG"
     gene            1094..5026
                     /locus_tag="PKK20_06805"
     CDS             1094..5026
                     /locus_tag="PKK20_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99631.1"
                     /translation="MKTRNISQSVLIGALAWLGIGQGNLAQSAEIANSITEFSGNQGQ
                     NGWFYGFRYFYDGLTVDYDVATEFIPFAGGVGQGDWDGFGQQWTGSAWDLNTSGAPWT
                     YLAAERVHPNGINNGEEHWVIRRWTASELGGNTWLGVQWNVRKENLAGTGVTGALHVN
                     GVRVQSAAVAGNDGVGVTQTYWVIAKSTDVFDVILSPVGPSGDTGDGADGSFTWMVVT
                     ATADTDGDGLPDAWEETYFPGDLTKLSGDGDYDADGLKDLAELQRGTDPTKSDTDADG
                     LIDSVETDTGIYVSPTDTGTSPIRADTDGDGRTDGDEVNGTPKTDPTDPDSDDDGYSD
                     GDEVASGHDPNDGTNNPDTTWIAHSRLEFSTDGSQGANDWYGGYRDYTHDGGGINYNP
                     DTAFIPFTPDTWTGGRWDLAPSTAPWTELGAESTHPAGTSPTHWTVRRWVATELTETT
                     PLALRWHVRKTNAGCGNGVTGALYINGERMDAVTLAFNNTTGVTRTFFANVKVGDKID
                     LILSPRGTDNGDADGCDGSANWLVIDPTIPPNPKQPDGSPFIPVGAGDTDADGMPDAW
                     EEIYFPGDLTKLTLNGDFDADGLKDPDEFARGSDPTNPDTDGDGLGDLVETGTGVYVS
                     PTDTGSSPTKIDSDGDGISDYAEVTRVPPTDPNKADTDGDGFSDLAEFEWGTNPVDPA
                     DHPLAQVIANSEKEFSGVQGRDNWYNGYREYDPETSTIDYDPNVDFIPFVGGEDAGAW
                     DGWSQTWTGSAWDLNTEGAGPWTYQAALNVHPNGANSPATIGGEIGLEHWVIRRWVNK
                     ELTAVTPVALIWSVKKENPANAGVTGSLHLNGSQIDHLTIDGTDTTGQIRRYYVNLKA
                     NDIVDLALTPEGVGGDRRDWSDGSTTWLWVDKKIPKEPRQPDGSLFIPANAQDTDADG
                     LPDFWELIYAADLTVLTGTGDNDGDGVVNTVEFARDSNPLVADTDGDGLSDAVETKTG
                     IFVSATDTGTDPRKADTDGDGRTDGEEVNGTPKTNPLLFDTDGDGFSDGAEVASGHDP
                     NDPNHNPNTTVIADSIVEFTTTGEQGVNGWFYGYRNLTSDGGAQNYNPDTAFIPFPAE
                     AWTGTQFDLDTSGAPWTELGRETTHPNAGTGDTHWTIRRWVATELTKVTPLGVRWHTR
                     HVNVACGGNGITGLLYVNGRLQDSAVIASTDGVGVIRTYYINAQPGDKIDLALSAKGT
                     DGLETDGCDGSANWFQVDPTIPPNPKQPDGTPFIPAGSQITITTAYNAAQSQLTLNWN
                     AVAGSTYTVWGTMDLKAWVQVQTGIAAGSHTIQIGVTPAYQFYRVSQP"
     gene            5595..7166
                     /locus_tag="PKK20_06810"
     CDS             5595..7166
                     /locus_tag="PKK20_06810"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNU99632.1"
                     /translation="MKNERIVIFDTTLRDGEQCPGASMNLREKLEVARQLARLKVDVI
                     EAGFPITSEGDFAAVQTIAREIKGPVIAGLARCVPADIDRAAAAVKPAGKRARIHVFL
                     ATSKIHREFKLNKAQSEIIRLSVEGVKRARSYVRDVEFSPEDGSRTEPEFLVQVCQAV
                     VAAGATTVNIPDTVGWAIPDQFGALIKQLHDAVPEFRSGKAVISVHCHDDLGLAVANS
                     LAAVRAGARQIECTVNGIGERAGNASLEELVMALRTRGDWFRGFQCGVNPREIVRSSR
                     LVSRMSGLAVQRNKAIVGENAFAHSSGIHQDGILKKRETYEIMDPQDVGWGTTELPLT
                     KHSGRAAVAARLRQLGFRMSDAEVQAIFTRFKEIGDKKKFVYDDDLTALAEDRITEVP
                     ETWSLDYLHVTSGSRTVPTATIQLRTAGRGKAVETVQDASIGDGPVDAVLKAIDRLTR
                     TKGKLLDYALRAVSQGMDALGEVTTKVDFGDGQVVTGRGASTDVIEASARSYLNAVNR
                     YLGSNAKCSSGNPQP"
     gene            7175..7678
                     /gene="sixA"
                     /locus_tag="PKK20_06815"
     CDS             7175..7678
                     /gene="sixA"
                     /locus_tag="PKK20_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNU99633.1"
                     /translation="MHLHLLRHGIAVKRNAARYRTDAERPLAPEGRQKLERIARALRA
                     MELEFDLILSSPAVRARETAEIVVKTLGTHQRIEFSEHLGTDGDLRRLIADLSARHPL
                     PRSALLVGHEPHLSGLASLLLTGGPGLDFVLKKGGLCTLGISKLRAGRCAGIDALLTP
                     RQMALMV"
BASE COUNT         1547 a         2586 c         2445 g         1256 t
ORIGIN      
        1 ggagcgctgc cagaacaatc cccaaatccc tttccagata ctcgtcaagc accgttccaa
       61 atgaaaagac cgtggagtca gcccccagag gccgcaggcg cgcttcggtg gcgctcgccg
      121 aagatcgcgg tcccgaccct cacaatcgtc gccccttcct cgatggcgac ctcgaaatcg
      181 ctgctcatgc ccatgctcag gtgttcgagg ggcgcaccca ggcgcgcttc gcattcggct
      241 ttgagttcgc gcaatcggcg aaacacagga cggacctttt ccggatcggg cgtccacggg
      301 gccatggtca tcaaaccgtg gatttcgagt cgcctcagcc cgttgagccg atcgaactcc
      361 gccagcaagg tctcgggtcg atagccgaac ttggacgctt ctcccgccac attgacctcg
      421 agcaggatcg gcattgtcct gcccgccttc tcggcgcacc gattcacctc ctcggccaga
      481 cgcaggctgt cgatgctctg gatcatctcg aagaagtgaa ccgcatcgcg ggccttgttg
      541 gtttgcagat ggccgatcat gtgccagcgc agccgcgcca gacagagagg aatcttcgcc
      601 ttggcttcct ggaccttgtt ctcgccgaag atatcgagcc ccagacccgc ggcggcgcgc
      661 accacctcgg gcggctggcc cttcgtcacg gctacaagcg tcacctcgga cgcctgccgc
      721 ccggcccggc ggcacgcctg tgcaacccgc tcctgcacct tccgcagtcc ctgagagagt
      781 tccatgccgg cgagggtaag aagccgtgag ggaaagacaa gcgccatgcg ctgtggcagg
      841 cgcttgatgg gagccgcacc gaggcaagga cccttacccg ggacacttct tagcgcggca
      901 aagccgcaat caaattgcgg ccacaactga cagccaaggt caattatgaa gtggagcggg
      961 cgacggacgc cagggctgtt ctcaggcttg acatcgctcg gagcgagatc agagttgcgc
     1021 ttgtccaatc cagtttgtga caggagtctg ttgcgctcgt tctgcagcca caacgtgtga
     1081 agaccagagt cttatgaaaa caagaaacat atcccaaagc gtcttgatcg gcgcgctcgc
     1141 ctggctggga atcggccagg gcaatctggc gcagtcggct gagatcgcaa attcaatcac
     1201 ggaattctcc ggcaaccagg gccagaacgg ctggttctac ggctttcgct acttctatga
     1261 cggcctcacc gtcgattacg atgtggcgac ggagtttatc ccgtttgctg gaggtgtagg
     1321 ccagggggat tgggacggct tcggccagca gtggacgggg agcgcctggg acctgaacac
     1381 ctcgggtgcg ccttggacgt atctggcggc cgaacgggtt catcccaacg gcatcaacaa
     1441 cggcgaggag cactgggtca tccgccgctg gacggccagc gaactgggcg gcaatacctg
     1501 gctgggcgtc cagtggaacg tccgcaagga gaacctcgcc gggacgggcg tgaccggcgc
     1561 gctgcacgtc aacggcgtgc gcgttcaaag cgctgccgtg gcgggcaacg acggcgttgg
     1621 cgtcacacag acctactggg tcatcgcgaa atccaccgac gtcttcgacg tgattctcag
     1681 ccccgtgggc ccgagcggag acaccggcga cggcgcggat ggttcgttca cgtggatggt
     1741 ggtgaccgcc acggctgaca ccgacggcga cggtctgccc gatgcatggg aggagactta
     1801 cttcccgggc gacctcacca agctgagcgg cgatggggac tacgacgcag acgggctcaa
     1861 ggacctcgcc gaactccagc gtggcacgga cccaaccaag tcagacaccg atgccgacgg
     1921 tctgatcgat agcgtcgaga cggacaccgg catctatgtc agtcccaccg acaccgggac
     1981 cagcccgatc agggccgata ccgatggcga cggtcgcacc gacggcgacg aggtcaacgg
     2041 aaccccgaag accgatccca ccgacccgga cagcgacgat gacggctaca gcgacggcga
     2101 cgaggtcgcc agcggccatg atcccaacga cggaaccaac aacccggaca ccacgtggat
     2161 cgcacactcg agactcgagt tcagcacgga cggctcgcaa ggagcgaatg actggtatgg
     2221 cggctaccgc gactacacgc acgacggcgg cggtatcaac tacaatcccg acaccgcctt
     2281 cattcctttc acgcccgata cctggaccgg cgggaggtgg gacctcgccc cgagcaccgc
     2341 gccctggacc gaacttggtg ccgagtcaac ccatccggcc ggcacatcgc ctacgcattg
     2401 gacggttcgc cgctgggtgg cgactgaatt gaccgagacc acgccgctgg ccctccgctg
     2461 gcacgtccgc aagaccaacg cgggctgcgg gaatggcgtg accggcgccc tgtacatcaa
     2521 cggcgagcgc atggacgcgg tcacactcgc gttcaacaac accaccggcg tgacccgcac
     2581 cttcttcgcc aatgtcaagg tcggcgataa gatcgatctc atcctcagtc cccggggcac
     2641 cgacaacggc gacgccgatg gctgcgacgg cagcgccaat tggctggtga tcgacccgac
     2701 catcccgccc aacccgaagc agccggacgg ctcgcccttc attcccgtgg gcgccggcga
     2761 caccgacgcc gacggcatgc ccgatgcgtg ggaggagatt tacttcccgg gtgacctgac
     2821 gaaactcacc ttgaacggcg actttgacgc cgatggtctg aaggacccgg acgaattcgc
     2881 gcgcggctcc gatcctacaa accccgacac ggacggcgac ggcctcggcg acttggtcga
     2941 gaccggcacc ggcgtgtacg tcagtccgac cgacacgggc agcagcccca ccaagattga
     3001 cagcgacggc gacggcatca gcgattacgc cgaggtgacc cgcgttccgc cgaccgatcc
     3061 gaacaaagcc gacaccgacg gtgatgggtt ctcggatctc gccgagttcg agtggggcac
     3121 gaaccccgtc gatcccgccg accatccact ggcccaggtg atcgccaatt cggagaagga
     3181 gttctcgggg gtgcagggcc gggacaactg gtacaacggc taccgtgaat acgatccgga
     3241 aaccagcacc atcgactacg atccgaacgt cgacttcatt cccttcgtgg gaggtgaaga
     3301 cgccggcgcg tgggatggct ggagccagac ttggacgggc agcgcctggg atctgaacac
     3361 cgagggggcc ggtccttgga cctaccaagc cgctctgaac gtccatccca acggggcgaa
     3421 cagccctgcc accatcggcg gcgaaattgg tctggagcat tgggtcatcc gccgctgggt
     3481 caacaaggaa ctcaccgcgg tcacccccgt ggctctgatc tggagtgtga agaaggagaa
     3541 tccggccaac gcgggcgtga cgggttcact ccacctcaac ggcagtcaga tcgatcacct
     3601 tacgatcgac ggaacggaca ccaccggtca aatccgccgc tactacgtca acctcaaggc
     3661 caacgacatc gtggatttgg cgctgactcc ggaaggcgtc ggcggcgatc gtcgcgattg
     3721 gagtgacggc tcgaccacct ggttatgggt cgacaagaag attcccaaag aaccccgcca
     3781 gcccgatggc tcgctcttca tccctgccaa cgcgcaggac acagacgccg acggtctgcc
     3841 cgacttctgg gagttgatct acgccgccga tctcacggtg ctcaccggca cgggcgacaa
     3901 cgacggcgac ggagtggtca acacggtgga atttgctcgc gacagcaatc cgctggtggc
     3961 tgacaccgac ggcgatggcc tgagcgatgc cgtcgagacc aagacgggca tattcgtcag
     4021 cgccaccgac accggcaccg atccgcgcaa ggcggatacc gacggcgatg ggcgcacgga
     4081 tggcgaggaa gtcaacggca cgcccaagac aaatccgctt ctgttcgata cggacggcga
     4141 cggtttcagc gacggcgccg aagtcgcgtc ggggcacgat ccgaacgatc cgaatcacaa
     4201 tccgaacacc accgtgatcg ccgattcgat cgtcgagttc accacgacgg gcgagcaggg
     4261 cgtcaatggt tggttctacg gataccgcaa cctgacctcg gacggcggcg cccagaacta
     4321 caatccggac acggccttca ttccgttccc cgcggaggcc tggaccggca cgcagtttga
     4381 tctggacacc agcggagcgc cctggaccga actcggacgg gagaccacac atccgaatgc
     4441 cgggacgggc gacacgcact ggacgatccg ccgctgggtg gccaccgagt tgaccaaagt
     4501 gacgccgctg ggcgtccgtt ggcatacacg gcacgtcaac gtcgcgtgcg gcggcaacgg
     4561 catcaccggc cttctgtacg tgaacggccg gctccaggac tcggcggtca tcgcttctac
     4621 ggacggtgtc ggcgtcatcc gcacctacta catcaacgcc caaccgggcg ataagatcga
     4681 ccttgcgctc agcgccaagg gaaccgatgg cctcgagacc gacggctgcg acggttcggc
     4741 caactggttc caggtcgatc caaccatccc acccaacccg aagcagccgg atggcacgcc
     4801 gtttatcccg gccggcagtc agatcacaat caccaccgcc tacaacgccg cccaaagcca
     4861 gctcaccttg aactggaatg cggtcgccgg ctccacctac acggtctggg ggaccatgga
     4921 cctcaaggcg tgggtccagg tccaaacagg cattgccgct ggctcccata ccatccagat
     4981 cggcgtgacg cctgcttacc agttctatcg cgtttcgcag ccttaagcga ttcatcgcgc
     5041 gcagagtcga ttcgatcgcg ccgcgctcgc cgggtcccat cccggtgggc gcggcctgtc
     5101 gttcggatcc gcatgagaac cgggtttgct tcccgcaagc ctcccctgcc tgccgcctgc
     5161 ccgcaggcag acagggcagc caggtcctcg tcgcccccca aacagggtct gcaactctca
     5221 tcgccgagga cgagaccgtc cccactccaa tgcagcgcag actcgattcc caccgacaac
     5281 ctcgaaatgc accgccgacc ttgacgatcg cgaataatca tttgactcgt ctgaggcttc
     5341 cttctaatgt ggccgcaacg atgaacggga ttgcgaatct ggctctgcgg acgctgctgc
     5401 tgttgtgcgg cgtggcggac gggttctaga gcaatcccca ggatttccgg agccccactg
     5461 ccacgcacgg cggtggggct ctctgttttg ggcccgcatc gccgtcgacg tcggtggtta
     5521 tgccccggag aatgcaacgg gcagatggcc cagacaacaa catgcagaga acgaacgaaa
     5581 agtgcaggaa caccatgaag aacgagcgca tcgtcatctt tgacacgaca ctccgcgacg
     5641 gcgagcagtg cccgggggcc tcgatgaatc tccgcgaaaa actcgaagtg gcccgccaac
     5701 tcgcccggct gaaggtggac gtgatcgagg ccggattccc catcaccagc gagggcgact
     5761 tcgccgccgt gcagaccatc gcgcgcgaga tcaagggccc tgtgattgcc ggcctggccc
     5821 ggtgcgtgcc ggcggacatc gaccgcgccg ccgcggcggt gaaaccggcg ggcaagcgag
     5881 cgcggattca cgtctttctc gccacatcga aaatccaccg cgagttcaaa ctgaacaagg
     5941 cgcagagcga gatcattcgc ctctcggtcg agggcgtgaa gcgggcgaga tcctacgtga
     6001 gggacgtcga gttctccccc gaggacggct cccgcaccga gccggagttt ctcgtgcagg
     6061 tgtgtcaggc cgttgtggcg gcgggcgcca ccacggtgaa catcccggac acggtcggct
     6121 gggcgatacc cgatcagttc ggcgcgctca tcaagcagct tcacgatgcg gtccccgagt
     6181 ttcggtcagg caaggcggtg atcagcgtgc actgccacga cgacctcggc ctggcggttg
     6241 cgaactccct tgccgcggtc cgggccggcg cgcggcagat cgagtgcaca gtcaacggca
     6301 tcggcgaacg ggccgggaac gcttcgctcg aggaattggt catggcgttg cgcacccgcg
     6361 gggattggtt tcgcggtttc cagtgcggcg tcaatccgcg cgaaatcgtc cgatcatcga
     6421 ggctcgtctc ccggatgagc ggtcttgcgg tccagcgcaa caaggcgatt gtcggcgaga
     6481 acgccttcgc ccactccagc ggcatccacc aggacggcat cctcaagaaa cgcgagacct
     6541 acgagatcat ggacccgcaa gacgttgggt ggggcaccac cgagttgccg ctgaccaagc
     6601 acagcgggcg cgccgcagtc gccgcccggc tgcggcagct cggatttcgc atgagcgacg
     6661 ccgaggtgca ggccatcttt acgcggttca aggagatcgg cgacaagaag aaattcgttt
     6721 acgacgacga tctcacggcc ctggccgaag accgcattac cgaggtgcct gaaacctggt
     6781 cgctcgatta tcttcatgtc accagcggca gccggaccgt gccgaccgcg acgatccaat
     6841 tgcgcaccgc ggggcgcggg aaggccgtcg agacggtcca ggatgcgagc atcggcgacg
     6901 gcccggtgga cgccgttctc aaggccatcg accgcctgac ccggacgaag gggaaactgc
     6961 tcgattacgc cttgcgcgcg gtgtcccagg gcatggatgc cttgggcgag gtgacaacca
     7021 aggtggattt cggcgacggc caggtggtga ccggcagggg cgcgagcacc gatgtcatcg
     7081 aggcgagcgc gcggtcatac ttgaacgccg tgaaccgata cctcggcagc aatgccaagt
     7141 gctcgagcgg caatcctcaa ccgtgatcgg aacgatgcat ctgcacctct tgcggcacgg
     7201 catcgcggtc aaacgcaatg cggcccggta ccgaaccgac gccgagcgcc cgctggcgcc
     7261 cgaggggcgc cagaaactgg agcgcatcgc ccgggcgctg cgggcgatgg aactcgagtt
     7321 cgatctcatc ctctcgagcc ccgcggtccg tgcccgtgag accgccgaaa tcgtcgtcaa
     7381 gacgctgggc acgcatcagc gcatcgagtt ctcggagcac ctggggaccg acggcgacct
     7441 gaggcggttg atcgctgact tgagcgcgcg gcacccgttg ccccgaagcg cgctgttggt
     7501 gggccacgag ccgcatctca gcggcctggc ctctcttctc ctgaccggcg gcccgggcct
     7561 cgattttgtg ctgaagaaag ggggcctctg cacgctcggc atctcgaagc ttcgggctgg
     7621 gcgctgcgcc ggcatcgacg cgctgctgac cccccgccaa atggccctga tggtctaggc
     7681 cggacatttc acacggctgc gcgcgtgact cggatcggtc agaggagtgt cgtcgtttat
     7741 ttttatgccc ttggcgggcg acgcgctcct tgtagttgac ccccgcagcc cggaccagag
     7801 gcatgctctg tggggaatct cccgcccctc acat
//
LOCUS       DAOQRO010000143         7819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8920_length_7819_cov_4.499356, whole genome shotgun sequence.
ACCESSION   DAOQRO010000143 DAOQRO010000000
VERSION     DAOQRO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_7819_cov_4.499356"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKK20_06820"
     CDS             <1..469
                     /locus_tag="PKK20_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99634.1"
                     /translation="TASRVSPERMRKIRVFSGGAVPSYVSLDYRAQSGYLCRLARSSE
                     ADASLWQRILAAREGTIISEFAGKRIVRESVPITHEEPLKLELQSFINCIRARQAPVV
                     TGEAAKHALDLAFEITRQIQAGIPSSWAGWESAAEPNPVAASSGDAPRERLGG"
     gene            494..1765
                     /gene="lpxB"
                     /locus_tag="PKK20_06825"
     CDS             494..1765
                     /gene="lpxB"
                     /locus_tag="PKK20_06825"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNU99635.1"
                     /translation="MRTNPPHGIMLVAGETSGDLLGAELVSALRRSPGWQAWPHEPRV
                     FGAGGPLMRAAGVDIALDLTRHAVVGLAEVVRRALEFRRLLQSLVDLACARQPDLLVG
                     IDFSAFNRRLVRALRRRLAAQTGVFHNWRPRFVQYVSPQVWASRPRRAYGLARDYDLL
                     LCLFSFEKEWYARRVPKLAVEWVGHPLLDRHATRLARPRASPPASPSDPPTILLLPGS
                     RVGELQAHLPVLLGAVERLRAGLRARFQLVLPNPGLRPLAERLGARDGPDFAVQVGGL
                     AEALDGATVAIASTGTVTLECALYGVPTVTFYKTSWTTYQFAKRLVTVAYAAMPNLLA
                     NAPVFPEFIQHAATPDNLADAARALLTDPARRNQIGQRLAQVVGSLGGPGASDRAAAA
                     ILGILNSPPRRRLSMGRDAPHDFLAPMGADL"
     gene            2238..3443
                     /locus_tag="PKK20_06830"
     CDS             2238..3443
                     /locus_tag="PKK20_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNU99636.1"
                     /translation="MQVYTRYRTAARRGRWRSLAHLALASAGVAFLGWVFWHTVRTLD
                     SQAPASATPKPATAAGAQAPAAHPPVPQTPAATNTARITVSTNLVVRTNPNPLLVSTP
                     ALPLDLEPVRDVARLQSPASVFEAQLALVRLGISPGVLDGVLGSQTRAALCVYQRRER
                     LPATAQLDSPTRARLGYAAPALTHRLVTFEDVAGLRSLSPTWLGKSRQDRLGYETVLE
                     LVSELSHSHPRFIRSLNPGIDWSRAGVGSLVVTPNAVRPSIETKADKVEISLGSRTLQ
                     VHDAGGQLLAHFPCSIARRVEKRPIGTLRVVMIIRDPSYTFDPKVFPESAEGRRLGRK
                     LLLPPGPNNPVGTAWIGLSRPGYGIHGTPHPEDVGRTESHGCFRLANWDAEHLADLVS
                     VGTLVVVGR"
     gene            3727..7146
                     /locus_tag="PKK20_06835"
     CDS             3727..7146
                     /locus_tag="PKK20_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99637.1"
                     /translation="MNAPTNQIVSLGLLAALTACFAAAVAQAQIPVLSTKDKVASRVG
                     VFRGGSTFTPDGLGHTGQVGDRAVDFGMGTGPVYVEDATFLNALAQNDEMSVALWVYK
                     YDTAAGSGFWMNSPSSNEGKRGFQAHIPWSNNNVYFDTAGCCTAPQRINASVDNWTGW
                     SGDWLMWHFLVFIKKGADKQIWIDGQLFLDQETANPGIAAAKLPTDFTDMYIGSDGAG
                     AGLFHALVDDFSIYGTALSEATIAQLYSGTLPTALPASDKLAAYWDFNDVPGEGIFLS
                     VSPAPNAGGAAPNLIRIVHVDGLVPWETGNVTLKVDGVTVTPNFDKDAITATLTYVPT
                     PAFTGHTTHKIALTYPGEAGTPVTAEWSFLVGPYTKDVVASRIGTFLDGSTFTADAGG
                     RTGQPGDLGADFGNLTGPVRVHDATFLNALAQNDVMCFAFWLKKPAIEAGSAFWANSA
                     SSAADLRGFQAHVPWSDGNIYFDSAGCCGAATERIYANISTFAYYSGEVSWWNDWHHF
                     VFQKNLSTKEIWIDGQYFLIGDNTDPLPTDFTELFIGAAASGVNRMSGVIDDFAIFGA
                     ALSETTIAQLADGTAPTALPASDKLTAYWSFNDFPSAGMFTTIDPTPNSQAARPDLVY
                     VRHMDGTVVWDQNKVSLKVDDTPVSYAFAKNGRLSDVSYVPDPWFQAGSTHKATLIYP
                     GEGAAQETLEWQFTIGTFEEVGTDLWTAPGTGDSTKPGFKARVWQIDQLGTVGLLNYV
                     RRAEQELAGIIGPNVADMTTAVEGVFNVDLVNWNQDHATTQIGNFQTGSTPSRPDAPI
                     PGIPGTGNTAYNTDNIAAEIIAYLEFPAAGFYQMGVNSDDGFRVSVGDTPPANNLALV
                     VSGAPSAAGSYHTAVAPSSTAKPFTAPISAKLVYMDPADGCTDLVNAAALSGNIALVD
                     RGTCDFSAKIKAAKDAGAIAVVVVNNRTEDLGAWPEGVFPIEMGAGAVGYQDIPAVMI
                     SSPDGDKIKTGLNSDLIASLTPDDTPALGVADFGRGSADTIFSFTVSKAGVYPFRCVW
                     FEGNGGANLEWFTVTPSGQKILINDTSNPAAIKAYRARTASPQPTLMLSVARSGNNLT
                     ITSNPQPLPAGFELQTAPSINGPWTTQAGANTPITVPIGTEQAVFLRAAKP"
     gene            complement(7399..>7819)
                     /locus_tag="PKK20_06840"
     CDS             complement(7399..>7819)
                     /locus_tag="PKK20_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99638.1"
                     /translation="KDTDPRRFQKPLPMSEAIAKARFWWNVAEVRQVFPTDDSMRLAL
                     DWLQRHHWAESASSIPNSPPRSGPLAFNASSPPTRLTSDCPDDRGTAGPRGARPSDRW
                     FPLVSPALRGGTTNDVFIGWEQARQSRNRRIAAAESR"
BASE COUNT         1360 a         2654 c         2388 g         1417 t
ORIGIN      
        1 caccgccagc cgggtcagcc cggagcggat gcgcaagatt cgcgtcttca gcggtggggc
       61 ggtgccgagc tacgtgtcgc tcgattaccg tgctcagtcg gggtacctct gccgcctggc
      121 gcgttcgagc gaggcggatg cgtcgctgtg gcagagaatc ctggcggcgc gggaagggac
      181 gattatcagc gaattcgcgg gcaagcggat tgtccgcgag tcggtgccga tcacgcacga
      241 ggaaccgctg aagctcgaac tccagagttt catcaactgt atccgcgccc gtcaggcccc
      301 cgtcgtcacc ggcgaagcgg ccaaacacgc acttgatctg gccttcgaaa tcacgcgcca
      361 gatccaggcc ggcatcccgt cctcctgggc cggatgggag tccgcggctg agcccaaccc
      421 cgtggcggcc tcatcgggcg atgcgccccg tgagagactc ggagggtgag tccccggacc
      481 gctgtaagtc cgcatgagaa ccaacccgcc tcacgggatc atgctggtgg ccggcgagac
      541 gagtggcgac ttgctgggcg ctgaactcgt gtccgctctg cgccgatcgc ccggatggca
      601 ggcctggcct cacgaacccc gggtgtttgg cgcgggcggc ccgctcatga gggccgcagg
      661 cgtggacatc gcgctggatc tcacccggca tgcggttgtc ggcttggctg aggtggtgcg
      721 gcgggctctc gaatttcggc gcttgcttca gagccttgtc gacttggcct gcgcgcgcca
      781 acccgacctg ctggttggga tcgatttctc cgcattcaat cgccgtctgg tgcgggctct
      841 caggcgccgg ttggcggcgc aaaccggggt cttccacaac tggcgcccac gttttgtgca
      901 gtacgtgtcg cctcaggtct gggcctcgcg accgcgacgc gcttacggtc tggcgcgcga
      961 ttatgacctg ctgctctgcc tgttctcgtt tgagaaggag tggtacgcgc ggcgggttcc
     1021 caaactcgcc gtcgaatggg tgggccatcc attgcttgac cgtcatgcca cgcgtttggc
     1081 ccgcccgcgt gcctcgcccc cagcctcccc gtccgacccg cccacgattc tcctcctgcc
     1141 gggcagccgc gtgggcgaac tgcaggcgca tctccccgtc ctgcttggcg cggtcgaacg
     1201 gctgcgtgcc ggtcttcgag cgcgattcca gctcgtcctg cccaatccgg gtctgcgtcc
     1261 tttggccgaa aggctcgggg cgcgcgacgg acccgatttc gcggtgcagg tgggcggttt
     1321 ggcggaggcg ctcgatgggg cgaccgtggc gatcgcatcg acgggcacgg tgactctcga
     1381 atgtgcgctg tacggcgtgc cgaccgtcac gttctacaag acctcgtgga caacgtatca
     1441 gttcgccaag cgcctggtca cggtcgccta tgcggccatg ccgaaccttc ttgcgaatgc
     1501 gcccgtcttt cccgagttca ttcagcacgc tgccacccca gacaacctcg ccgacgcggc
     1561 gcgcgcgctc ctcacggacc cggcccgccg caatcagatc gggcaacgcc tcgcgcaagt
     1621 cgtcgggtcc ttgggcggtc ctggggccag cgatcgggcg gccgcggcca tcctcggcat
     1681 tctgaattcg cctccgcgac ggcggctctc gatggggcgg gatgcgccgc atgatttcct
     1741 cgccccgatg ggcgccgatt tgtaggatgc agcccatgac acgtcattgc catcagtgtg
     1801 gctgggaatg gacgcacggg cgccagcccg ggcggaccga ggcttgcgag cggtgcgggg
     1861 cggaaatgcg ggtatgcctg aactgccatc attttgatct ggccgttgcc caacaatgcc
     1921 gcgagtcgcg agccgagcct gtcgcggaga aggatcgggc gaacttctgt gagtggttcg
     1981 atttcgcatg tcgtgtttat gcgcctcggg gcgggcgcga tccagcctcg gacgcgcgcg
     2041 ctcaattcca gaaactcttc ggagattgag gaactgctgc tccagcgcgc gcgatgttta
     2101 tacggagcga catgaatgat gcagcgccgg gtcaggcctg cctgccggca ggcagggcat
     2161 ccggtctgca agcctggagg ctgcgtgccc ccacgcggcg gaccttctga cgctgtgtct
     2221 agaagctgag ctgcgccgtg caggtctaca cccggtacag aaccgcggcg cgccgcgggc
     2281 gttggcgttc cttggcccat ctggccctcg cgagcgccgg agtcgccttt ctgggctggg
     2341 tcttctggca caccgtgcgg acgctcgatt ctcaggcgcc tgcttctgca acacccaagc
     2401 ccgccactgc ggctggcgcc caggcccccg ccgcacaccc ccccgtcccg caaacaccag
     2461 ccgccacgaa cacggcgcgc atcaccgtct ccaccaatct cgtcgtgcgg acgaatccca
     2521 atccgctcct ggtgtcgacg ccggcgctcc cccttgacct cgagcctgtt cgcgatgtcg
     2581 cccggctgca gtctcccgcc agtgtcttcg aagcccaact tgcactggtg cgtctgggca
     2641 tttcacccgg ggtgttggac ggcgttctcg ggtcgcagac gcgggcggcc ctgtgcgtgt
     2701 accaacgccg ggaacgtctg cccgcgaccg cccagctcga ttcccccaca cgggcccgcc
     2761 ttggttatgc ggcgcctgcc ctgacgcatc gcctggtgac gttcgaggac gtggccgggt
     2821 tgcgctcgtt gagcccgacc tggctgggca aatcgcgcca ggaccgcctc ggctacgaga
     2881 ccgtcctcga gttggtgtcc gaactctcgc attcgcaccc gagatttatc cgcagtctga
     2941 atcccgggat cgactggagc agggctgggg ttggatcgct ggttgtcacc cccaatgccg
     3001 ttcggccttc catcgaaacc aaggccgaca aagtggaaat ctccctcggt tccaggacgc
     3061 ttcaggtgca cgatgccggc ggacaactgc tggcgcactt cccgtgcagc atcgcccgcc
     3121 gggtcgagaa gcgccccata ggaacactcc gggttgtgat gatcatacgc gacccgagct
     3181 acaccttcga tccaaaggtc ttccccgaat cagccgaggg acggcgcctc gggcgcaagc
     3241 tgcttctgcc gccaggcccg aacaacccgg tcggcaccgc ctggatcggt ctgagccgcc
     3301 ccggctacgg catccacggg acgccgcacc ccgaggatgt ggggcggacg gaatcgcacg
     3361 gctgctttcg gctggccaac tgggatgccg aacacctggc cgatctggtc agtgtgggaa
     3421 cgctggtcgt tgtcgggcgc tagcccgctt ccgccctcct cggacaggac gcgggacttg
     3481 cctgaacaac tgtttgttcg ctcattcttg catgctgttg tgtatgtcgg gcggcgatca
     3541 aaactgcatg atgttttgta ttgcattcgc gccccgcggt gatcagtctc acctcgcttc
     3601 cactgcggcg gctatgccac tggatccagt gacgtgtttt tccgccagga cggctcgtgg
     3661 atcaggcaat cggaaaggcg aagctcgtca gcttcatgcc atgaatgaac ggtcaaccct
     3721 aataccatga atgccccaac caatcaaatc gtatcgctcg gtttgctcgc cgctctgacg
     3781 gcatgttttg ccgccgccgt ggcgcaagcc caaatccccg tcctctccac aaaagacaaa
     3841 gtcgcctccc gcgtcggcgt ctttcgcggc ggatccactt tcacgccgga tggcctcggc
     3901 cacaccgggc aggtcggcga tcgcgcggtt gacttcggga tgggcaccgg gcctgtgtac
     3961 gtcgaggatg cgacgttcct gaatgctctt gcccagaatg acgagatgtc ggttgcgctc
     4021 tgggtctaca agtacgacac cgccgctggc tcgggctttt ggatgaactc gccttcgagc
     4081 aacgaaggca agcgcggttt tcaggcccac atcccctgga gcaacaacaa tgtctacttc
     4141 gacaccgccg gctgctgcac cgcaccccag cgcatcaacg ccagcgtcga caattggacc
     4201 ggttggtcgg gcgattggct gatgtggcac ttcctggtct tcatcaagaa gggcgccgac
     4261 aagcaaatct ggatcgacgg ccagttgttc ctcgaccagg agaccgccaa tcccggcatc
     4321 gccgccgcca agttgccgac cgacttcacc gacatgtaca tcgggtccga cggcgccggc
     4381 gcaggtctgt tccatgcgct ggtcgatgac ttctcgattt acgggaccgc actgagcgag
     4441 gcgacgatcg cccaactcta cagcggcacg cttccgaccg ccttgccggc gagcgataaa
     4501 ctcgccgcct actgggattt caacgacgtt ccgggcgaag gcatatttct ctccgtcagc
     4561 ccggctccga acgccggcgg cgccgccccc aatctgatcc ggatcgtcca tgtcgatggc
     4621 ttggtccctt gggagacagg gaatgtgacc ctcaaggtgg acggcgttac agtgacgccc
     4681 aatttcgaca aagacgcgat cacagccacc ctgacctacg ttcccacccc tgcgttcaca
     4741 gggcacacga cccacaagat cgcactcacg tacccgggtg aagcgggaac accagtcacc
     4801 gcggaatgga gtttcctagt cggcccctac accaaagacg tcgtggcatc ccggatcggc
     4861 accttcctcg atggctcgac cttcacagcg gacgccggag gccgcaccgg ccagccggga
     4921 gatctgggcg ccgacttcgg caacctgacc ggtccggtcc gagtccacga cgcgacattc
     4981 ctcaacgccc tggcccaaaa cgatgtgatg tgcttcgcct tctggctcaa gaaacccgcc
     5041 atcgaggccg ggtccgcctt ttgggccaac tcagcctcca gcgcggcgga cctgcgcggt
     5101 ttccaggcgc acgtcccgtg gagcgacggc aacatctatt tcgactcggc cggctgctgc
     5161 ggcgccgcaa ccgaacgcat ttacgccaac atcagtacct tcgcctacta ctcgggcgag
     5221 gtctcctggt ggaacgactg gcatcacttc gtctttcaga agaacctgtc gacaaaggaa
     5281 atctggatcg acggccagta cttcctgatc ggcgacaaca ccgacccgct gccaaccgac
     5341 ttcaccgaac tgttcatcgg cgccgccgcc tcaggagtca atcgcatgag cggtgtcatc
     5401 gacgatttcg ccatcttcgg agccgccttg agcgaaacca caatcgccca gctcgccgac
     5461 ggcacggcgc cgacggcgct ccccgcctcc gacaaactca ccgcgtactg gtccttcaac
     5521 gacttcccgt ccgcgggaat gtttacgacc atcgacccca cgcctaattc acaggcggcg
     5581 cggcctgatc tcgtctatgt gcgccacatg gacggcactg tcgtctggga ccaaaacaaa
     5641 gtgagcctga aggtggatga cacaccggtg agttacgcgt ttgccaagaa tggccggctg
     5701 tccgatgtca gctatgtccc cgatccctgg tttcaagccg gctcgactca caaggccacc
     5761 ctaatctatc cgggcgaggg cgccgcacag gaaaccctcg aatggcagtt caccatcgga
     5821 accttcgaag aggtcggcac tgacctctgg accgcgcccg ggactggcga ctcgaccaag
     5881 ccaggtttca aggcgcgcgt ttggcagatc gaccaactgg gcacggtggg actcctcaac
     5941 tacgtccggc gggcggagca ggaactggcc ggaattatcg ggcccaacgt ggccgacatg
     6001 accaccgcag tcgagggcgt gttcaacgtc gacctcgtca actggaatca agaccacgcg
     6061 acaacccaaa tcggcaattt ccagacgggc agcaccccca gccggcccga cgctcccatc
     6121 ccgggcatcc ccggcacggg gaacactgcc tacaacaccg acaatatcgc ggccgaaatc
     6181 atcgcctacc tcgagttccc tgccgccgga ttctatcaga tgggcgtcaa cagcgatgac
     6241 ggcttccgag tcagcgtggg cgacacaccg ccggccaaca acctggccct cgtcgtgagc
     6301 ggcgccccct cggctgcggg cagctaccat acagccgtgg ccccctcgag cacggccaag
     6361 ccattcaccg cccccatcag cgccaagctg gtgtacatgg acccggctga cggatgcacc
     6421 gatctggtca acgccgccgc tctgagcggg aacatcgccc tggtggaccg cggcacttgc
     6481 gacttcagtg ccaagatcaa ggcagccaag gacgccggcg ccatcgcggt cgtcgtggtg
     6541 aataatcgaa ccgaagatct cggggcctgg ccggaaggag tgttcccgat cgaaatgggc
     6601 gcaggcgcgg tcggttacca ggacattccc gccgtgatga tctcgagtcc cgatggcgac
     6661 aagatcaaga ccggcttgaa cagcgatctg atcgcttccc ttacgcccga tgacaccccg
     6721 gccctcggtg tggccgactt tggacgagga tcggccgaca cgatcttctc cttcacggtc
     6781 tccaaggcgg gagtctatcc gttccggtgc gtctggttcg aaggcaacgg cggcgcaaat
     6841 ctcgagtggt tcaccgtcac cccctccggc cagaaaatcc tgatcaacga cacgtcgaat
     6901 cccgcggcga tcaaggctta ccgggcccgc accgcgtcgc cacagccaac actcatgctc
     6961 agcgtcgccc gctcgggcaa caacctgacc atcacgtcca acccgcaacc gctgccggcc
     7021 ggattcgaac tgcagaccgc accctcgatc aacggtccgt ggacaactca agcgggcgcg
     7081 aacaccccga tcaccgtgcc catcggcacc gaacaggccg tgttcctccg cgccgccaaa
     7141 ccttagcgct tcgattcaca aatacgatcg cgtatccgga ctgtgcaacg ccgagtttcg
     7201 ataactcggc actcagtctt tcagccatga gttcgtcacc gaacttatgg atcgaaggac
     7261 ttggtgagcg gtccgcacag cgcatggcac gccccctcca ccccaccgtt cgcctcgtct
     7321 gatggggaaa gaggacaccc tgtggctgtg ggggggcagg tgaaggggcg cgtgtcgcgc
     7381 atccaggcct ctgggctctc agcggctctc cgccgcggcg atccttcggt tgcggctttg
     7441 ccgcgcctgt tcccacccga taaagacatc gttcgtcgtg cccccgcgca gcgctggcga
     7501 aactaacgga aaccaacggt ccgagggccg agctccgcga ggccctgctg ttccgcggtc
     7561 atccgggcag tcggaagtca gccgggttgg aggtgatgag gcgttgaatg ccagcggtcc
     7621 agagcgtggc ggtgagttgg gtatcgagga tgcgctttcg gcccagtggt ggcgttgcag
     7681 ccaatcgagc gccaggcgca tcgagtcgtc ggtggggaag acctggcgga cctcggcgac
     7741 gttccaccag aatcgggcct tggcgatggc ttccgacatg ggcagcggct tctggaatcg
     7801 ccgcgggtcg gtgtccttg
//
LOCUS       DAOQRO010000144         7778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_8995_length_7778_cov_6.210928, whole genome shotgun sequence.
ACCESSION   DAOQRO010000144 DAOQRO010000000
VERSION     DAOQRO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8995_length_7778_cov_6.210928"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKK20_06845"
     CDS             <1..374
                     /locus_tag="PKK20_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99639.1"
                     /translation="GQSHRIGTTFEGQDGIIYVDRGKLESSVKGAIEEPLGQDEVHLT
                     VSRDHHQNWFDAIKSRKPPICDVAIGHRSATVCHLGNIAVQTGKPLKWDPAKEQIVGD
                     PVQVKQYSYEYRKPWALPKIG"
     gene            463..1614
                     /locus_tag="PKK20_06850"
     CDS             463..1614
                     /locus_tag="PKK20_06850"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNU99640.1"
                     /translation="MKSRAIPLCSLAAVIGFLGVAPVRGQPLLQQTDVFASGTEGYVA
                     YRIPAIETAPDGTLLAFAEARKHNLGDPGYDDNDIDLVCRRSRDAGVTWSPMKIIEDP
                     GDRWSAANPATLVDRQKKRIWLFYLRCKPGRNTSTARPGTDDSQILARTSDDNGASWS
                     EPIDLTGVTRDLADPKWRCSVVGPGGGIETRDGRLVIPVWMFEPFAVFAVFSTDHGRT
                     WQRGQRVPVISGDECQLVELHDGRLMIDIRQQSGPHRWLSTSSDGGQTWSPARPGEKV
                     TPVCCAIERYPAPVDGEGGDWILWTGPKGPDRANLVVRISRDEGLTFPWERAVAPGLA
                     AYSDLAVLRDGTVGVLWERGAERGYQFITFTRFNRDWLAGPTSEEDQRP"
     gene            1611..2867
                     /locus_tag="PKK20_06855"
     CDS             1611..2867
                     /locus_tag="PKK20_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNU99641.1"
                     /translation="MSTPVTEFRRNALAAMVDRAVNSTPVIDIHTHLYDPLLGGLLLW
                     GIDELLVYHYLMAEVNRRQKLLPESFLTLRKSRQAEIIWNVLFIQHSPLSEACRGVLT
                     TLHRLGLDVKRRDLPALRDWFAQWNAETYVTRCMELANVKSIYMTNSPFDDEERAAWQ
                     KGFLRDERFVAALRVDPLLVDWPRAARRLAEWGYEVGEGLSTHTLNEVRRFLADWTLR
                     MEARYLMVSLPPEFSCDDDNECAQLMKAAVLPHCLEFGQPFALMMGVRRAVNPQLGLA
                     GDGLGRSDLGTLAHLCAAYPENRFLVTVLSRENQHELCVLARKFSNLHPFGCWWFNNV
                     PYLIDETLRLRLELLGTSFTAQHSDARVLDQIIYKWDHTRRSLSSALTDKYADLALTG
                     WEPTAAEVERDVQGLLGGAFEQFCKM"
     gene            2997..3143
                     /locus_tag="PKK20_06860"
     CDS             2997..3143
                     /locus_tag="PKK20_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99642.1"
                     /translation="MKQDEEDVLRVFDPKIGAFVAYNRDAETQLRRLASPGSRRSARR
                     PRRR"
     gene            3314..4276
                     /locus_tag="PKK20_06865"
     CDS             3314..4276
                     /locus_tag="PKK20_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99643.1"
                     /translation="MANSLNRRRFIQVSALGAAALPAVSRAQQDAAPAASASQAGPLA
                     SPKNTLPTGRIGDVAYSRLMLGGNLLSGYAHSRDLAYVAELMRRYNTDAKILETLETA
                     ELHGINVINSWVRDGISQLQAHWKRGGKMKWIAQARVNPNGDLDQFKQAADQGASAIH
                     ITGDGADALVQQGKMDTLVRILDLVRSYKLSAGIGAHGLRTIVECEKAKINPDFYVKT
                     LHTHDYHTAPKPGETDDLGRYDNSWCSNPEEVGDAMFNVTKPWIAFKVLAAGAIPPQR
                     GFRYAFENGADFILVGMFDWQIAEDVQITKQVLTGLQRLRPWRS"
     gene            4646..6253
                     /gene="dacB"
                     /locus_tag="PKK20_06870"
     CDS             4646..6253
                     /gene="dacB"
                     /locus_tag="PKK20_06870"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNU99644.1"
                     /translation="MSMLPMNHAGLSTPFFLLLAWLAWTAAAPALSSEVIPAPSDPAG
                     LRTRFAAHVNQDRFRHAHWGVRIVSIDSGQPVFEHNADDLLIPASNTKLFTGALALDR
                     LGPEFRIRTSVCSQERPTRRGTLKGDLILYGRGDPTFAARFHEGRLDRALEPLLGVVA
                     ATGIKRVRGDLVVDAGYFASPALGSGWDWDDLQYAYGAEVSALSINDNTVDVVVKPAQ
                     TPGRPATVQLVPPSGFIAVDNRCVTAPTGVRRAVQVERPLSHNLIVVTGRIPVGDAGI
                     TETVSVHAPAAWFGHLFKAALEKRGIRVAGNVRMTDPAGAPADSASGANRVELAAVES
                     PPLRDLVARMMKPSQNLYAQLLLLQVGAAELAASSQTNATRSAWPPAGVFPHTAEAAG
                     IRALETFLARAGIPADEVLFEEGSGLSRRHLVTPAAIVQLLRFMDRHPQAAVFRDALP
                     VAGIDGTLRNRMLETPAAGNARAKTGTLRYVNALSGYVTSKAGERFAFALLLNNFRNP
                     DSSRSPRTDLDEFVTTLAALEWRTDRQ"
     gene            complement(6260..6805)
                     /locus_tag="PKK20_06875"
     CDS             complement(6260..6805)
                     /locus_tag="PKK20_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNU99645.1"
                     /translation="MQSVTGPVYVVRDNIDTDQIIPAQYLNLVPTIAEEYEKLGSYAL
                     CGLPDALYPIRFVSSGQLDAEYPIIVAGRNFGCGSSREHAPIALGSAGCRIVVAESFA
                     RIFFRNSVATGELYPCECAERLCEVLKTGDMATVDLDACTVTCAAGGRVFRLKALGDV
                     RPVVDAGGLFNYARKTGMIAS"
     gene            6959..7327
                     /locus_tag="PKK20_06880"
     CDS             6959..7327
                     /locus_tag="PKK20_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNU99646.1"
                     /translation="MKRPMLYLYAGLIGLIGGVTSGLFGVGGGIVMVPAMMLLLQLDI
                     KSAIGTSLAVIVPTALIGTFKHHGLGHVDWRLALALAPTAVLGGFVGSWLTKFIASAD
                     LKRAFGGFLILVGARLLLFK"
BASE COUNT         1414 a         2591 c         2413 g         1360 t
ORIGIN      
        1 gcggccagag ccaccgcatc ggcaccacgt tcgaaggcca ggacggcatc atctatgtcg
       61 accgcggcaa gctcgaatcc tcggtcaaag gcgccatcga agaaccgctc gggcaggacg
      121 aagtccacct caccgtcagc cgcgaccacc accagaactg gttcgacgcc atcaagagcc
      181 gcaagccgcc catctgcgat gtcgccatcg gccatcgctc agccaccgtg tgtcacctgg
      241 gcaacatcgc cgtccagacc ggcaagcccc tcaagtggga tccggccaaa gagcagatcg
      301 tcggcgaccc cgtccaggtc aagcaataca gctacgagta ccgcaaaccg tgggcactgc
      361 ccaaaatcgg ataaccccgt cggctcgagc gcgctttggg agcggggtga gatgagacgt
      421 agagcccggg tcttaaaccg cgtccggccg tgttgacgag ctatgaagtc acgagccatt
      481 ccgctctgct cgcttgccgc tgtcatcggc ttcctcggcg tcgccccagt ccgcggacag
      541 cccctgcttc agcaaacgga cgtcttcgcc tcggggacgg aggggtacgt cgcataccgc
      601 atcccggcca tcgagacggc cccggacggc acgctgctcg ccttcgccga agcccgcaaa
      661 cacaacctgg gcgacccggg ctacgacgac aacgacatcg atctggtctg ccggcgcagc
      721 agggacgccg gcgtaacctg gtcgccgatg aagatcatcg aagaccccgg cgatcgctgg
      781 tcagccgcca atccggcgac tctcgtcgac aggcagaaga aacgaatctg gctcttctac
      841 ctccggtgca aaccgggccg aaacacctcg acggcacgcc ccggaaccga cgacagccaa
      901 atcctggcgc gcaccagcga cgacaacggc gcctcctggt ccgaaccaat cgacctcaca
      961 ggcgtgacgc gcgacctggc ggaccccaag tggcgttgca gcgtcgtggg gccgggcggc
     1021 ggcattgaga cccgcgatgg acgactcgtc ataccggttt ggatgttcga gcctttcgca
     1081 gtttttgccg ttttcagcac ggaccacggc cgcacgtggc agcgaggcca aagggtgccg
     1141 gtcatctcgg gcgacgagtg ccaactcgtc gagctgcacg acggccggtt gatgatcgac
     1201 atccgacagc aatccggccc ccaccgctgg ctctcaacca gcagcgacgg agggcagact
     1261 tggtctccag cccgcccggg cgagaaggtc acaccggtgt gttgcgccat cgagcgttac
     1321 ccggccccgg tcgatggaga gggtggcgac tggattctgt ggaccggccc caaaggcccc
     1381 gaccgggcga atctcgtcgt tcggatcagc cgggacgagg ggttgacctt tccgtgggaa
     1441 cgggcggtcg cacccggact ggcggcgtac tccgaccttg cggtcctccg ggatgggacc
     1501 gttggcgttc tgtgggaacg gggcgccgag cggggatacc agttcatcac cttcacacgg
     1561 ttcaatcgcg attggctggc tggcccgacg tcggaggaag atcagcgccc atgagcaccc
     1621 ccgtcacgga attccgacgc aacgctctgg ctgcgatggt cgaccgggcg gtcaactcga
     1681 cacccgtcat cgatatccac acgcatctgt acgatccctt gttgggcggg ctgctcctgt
     1741 gggggatcga cgagttgctc gtctaccatt acctgatggc ggaagtcaac cgccgccaga
     1801 aactcctgcc cgagagcttc ctgaccctgc gcaaaagccg ccaggccgag atcatctgga
     1861 acgtcctctt catccaacat tcgcccctct ccgaagcctg ccgcggcgtc ctgacgacgc
     1921 tccatcggct tggattggat gtgaagcggc gcgacctgcc cgcgctgcgc gattggttcg
     1981 cgcaatggaa tgccgaaacc tacgtcacgc gctgcatgga actcgccaat gtcaaatcga
     2041 tctacatgac caactcgccc ttcgatgacg aagagcgagc cgcttggcag aagggtttcc
     2101 tgcgcgacga acggttcgtc gcggcgctcc gggtcgatcc cctcctggtc gactggcccc
     2161 gagccgcgcg ccgcctcgcc gagtggggct acgaggtggg cgaaggcctc tcgactcaca
     2221 cgctcaatga agtccgccgc tttcttgcgg attggaccct gcggatggag gcgcgctacc
     2281 tgatggtttc tctgccgccg gagttctcct gcgacgacga caacgagtgc gcccaattga
     2341 tgaaagcggc cgtcctgccc cactgcctcg agttcgggca gccgttcgcg ctgatgatgg
     2401 gcgtgcggcg cgcggtcaat ccgcaactcg gccttgccgg agacgggctc gggcgcagcg
     2461 acctcgggac gttggcccac ctgtgtgccg cctacccgga gaaccggttc ctcgtcaccg
     2521 tgctgtcccg cgaaaaccag cacgagttgt gtgtcctggc ccgcaagttc tccaacttgc
     2581 atcccttcgg ctgctggtgg ttcaacaacg tcccctacct gatcgacgag acgctgcggc
     2641 tccggctcga gttgctcggg acaagcttca cagcccagca ctccgacgcg cgtgtgctcg
     2701 accagatcat ctacaagtgg gaccatacgc ggcgcagcct gtcgagtgcc ctgaccgaca
     2761 agtacgccga cctggccttg acaggctggg aaccgacagc ggcggaggtc gaacgggatg
     2821 ttcaaggcct cctgggcggc gccttcgaac agttctgcaa gatgtagtcc gaggcggatc
     2881 acatgtcaga tacacgccct gtcatccgtc tgtaggaaag cagtcctgat ctccctggtc
     2941 tgggcgttgt tgatggcccg gcagtcctcc aacgtgccaa ggcccaggac ctgccgatga
     3001 agcaggacga ggaggacgtg ctgcgcgtgt tcgatccgaa gatcggcgcc ttcgtcgcct
     3061 acaatcggga tgccgagacc caactccgcc gcctcgcttc acccggctcg cgccgatccg
     3121 cccgccggcc gcggcgccgt tgagcgacgg ggggcgggag tcggcgcaga tactggagcc
     3181 gacggaattc cggctcgggc gccggcttat gttcttatgt tcgagttcct tttttcgtgc
     3241 tgttcattgc cgagcgtccg ttctacactg cgcgccaatc gactgagtga tcgcacatga
     3301 ggaacacatg actatggcca attctctcaa ccgacgccgc ttcattcagg tctccgccct
     3361 cggcgccgcc gcgttgcccg ctgtgtcgcg ggcccaacaa gatgccgccc cggcggcgtc
     3421 cgcttcccaa gccggccctc tggcgtcccc gaagaacacg ctgccgactg ggcggattgg
     3481 cgacgtcgct tacagtaggc tcatgctcgg ggggaacctg ctcagcggct atgcccattc
     3541 ccgcgatctc gcgtacgtgg ccgagttgat gcggcgttac aacaccgacg ccaagattct
     3601 cgagaccctc gaaacggccg aattgcacgg aatcaacgtg atcaatagct gggtgcgcga
     3661 cggcatcagc cagttgcagg cgcattggaa acgcggcggc aagatgaaat ggatcgccca
     3721 ggcgcgcgtg aaccccaacg gcgatctcga tcagttcaaa caagcggccg accagggcgc
     3781 ctcggccatt cacatcaccg gcgacggtgc cgatgcgctc gtccagcagg ggaagatgga
     3841 caccctggtg cggattctcg atctggtgcg cagctataag ctgagcgcgg gcatcggcgc
     3901 gcatgggttg cgcacgatcg tcgagtgcga gaaggccaag atcaaccccg atttctatgt
     3961 caagaccctc cacacgcacg attaccacac cgcacccaag ccgggcgaga cggacgatct
     4021 gggacgctac gacaactcct ggtgcagcaa tcccgaggaa gtcggcgatg ccatgttcaa
     4081 cgtgaccaag ccctggatcg ccttcaaagt gctggccgcc ggcgccatcc cgccccagcg
     4141 cggcttccgt tacgccttcg agaacggcgc cgattttatt ctcgtgggca tgttcgactg
     4201 gcagatcgcg gaagatgtgc agatcaccaa acaggtcctc accggtctgc agcgccttcg
     4261 cccctggaga agttagcaag agggccgact gccacaacga acgccggccc aagggcttgg
     4321 acaaccacga ctgcgctgct cccaggaaca gcaggggcga tctctcgagg ttggcgcgcc
     4381 tgcgcggccg cgggagcgcc cctccgagta tggtgtatgg acccaatgcc ttcttcaaaa
     4441 caggaacctc ccaaaaagga acaggccgcc tcccatgaac cgcctgcgca agccgttgat
     4501 aatcaacaaa aggggtaacc cgaacgtcca acgcgcaacg ttcaacatcc aacttttaac
     4561 gaaccgcttc gttgaacgtt gagcgttgga agttgggcgt tgaacgttcc ccggttcatg
     4621 ggtccaatgc gtattaaacc gttgaatgtc aatgctaccc atgaaccacg ccggactttc
     4681 cacaccgttt ttccttctcc ttgcctggct ggcatggact gccgcggccc cagccctgag
     4741 ttcggaggtc atccccgcgc cgtccgaccc agcgggtctc cggacgcgat tcgcggcgca
     4801 cgtcaatcag gaccgctttc gccacgcgca ttggggcgtg cggatcgtct cgatcgactc
     4861 cggccaaccg gtgttcgaac ataacgccga tgatctgctg attccggcgt cgaacaccaa
     4921 gctgttcacc ggcgcgttgg ccctggatcg actcggaccc gagttccgca ttcgcacttc
     4981 ggtctgcagc caggagcgcc cgacgcgacg cggaacgctc aagggcgact tgatcctcta
     5041 cggtcggggc gatccgacgt tcgccgcccg gtttcatgag ggtcggctcg atcgggcgct
     5101 cgagcctctg ctcggggtgg tcgccgcgac cggcatcaag cgcgtaagag gcgacctcgt
     5161 cgtcgatgcc ggctacttcg ccagcccggc gctcgggtcg ggatgggact gggacgacct
     5221 ccagtacgcc tacggggccg aagtgtcagc cctctcgatc aatgacaaca cggttgatgt
     5281 cgtggtcaag ccggcccaga cgccgggacg accggcgacg gtccaactcg tgcccccgtc
     5341 cggtttcatc gccgtcgata accgatgtgt cacggcgccg actggcgtcc ggcgcgccgt
     5401 tcaggtcgag cgccccctga gccacaacct gatcgtcgtg accggccgca ttcccgtggg
     5461 cgacgcggga atcaccgaaa cggtctcggt gcatgcgccc gcggcgtggt tcggccactt
     5521 gttcaaggca gccctcgaaa agcgcggcat tcgcgtggcc ggcaacgtcc gcatgaccga
     5581 tccagccggc gcacccgccg attcggcgtc tggagcaaat cgagtggaat tggccgctgt
     5641 ggaatcgcct cccctccgcg acctagtggc gcgaatgatg aagccgtcgc agaacctcta
     5701 cgcccagttg ctgctgttgc aagtcggcgc cgcggaactg gcggcttcga gtcagaccaa
     5761 cgccacccgg agcgcctggc cgcccgcggg tgtgttcccg cacacggctg aagcggctgg
     5821 gatcagggcg ctcgaaacct tcctcgcccg cgcgggcatt cccgcagacg aggtgctgtt
     5881 cgaggagggc tccggcttga gccgccgcca tttggtcacg ccagccgcca tcgtccagtt
     5941 gctccgtttc atggaccggc atcctcaggc tgcggtgttc cgcgacgcgc tgcccgtggc
     6001 gggtattgac ggcacattgc ggaatcggat gcttgagacg cctgcggcgg gcaatgcccg
     6061 ggccaaaacc ggcacgttgc ggtacgtcaa tgccctgtcc ggttacgtca catcgaaggc
     6121 cggcgagcga ttcgccttcg ccctgctcct caacaatttc cgcaaccccg actcgagccg
     6181 atccccgcgc accgatctgg acgaattcgt gacgacgctg gccgctctgg aatggcggac
     6241 ggatcgccaa tgagcaggct caagaggcga tcatgcccgt cttgcgggcg tagttgaaca
     6301 ggccgccggc gtccaccacc ggacgtacat cgcccaaggc tttgagccgg aagacgcgcc
     6361 cgcccgccgc gcaggtcacc gtgcacgcat cgagatcgac tgtcgccata tctccggtct
     6421 tgaggacttc gcacaggcgc tccgcgcatt cgcacggata cagctcgccc gtggccaccg
     6481 agttgcggaa gaagatgcgg gcgaagctct ccgccaccac gattcggcac ccggcggacc
     6541 cgagggcaat gggcgcatgc tcgcgcgagc tgccgcagcc gaagttgcgc cccgccacta
     6601 tgatcggata ctccgcatcg agctggccgg aagagacgaa tcggatcgga taaagcgcgt
     6661 cgggaaggcc gcacagggcg tacgaaccga gcttctcgta ctcttcggcg atcgtcggca
     6721 ccagattcaa atactgcgcc ggaatgatct ggtcggtgtc gatgttgtcg cgcaccacat
     6781 agacgggacc ggtgacactt tgcatgggct ggacgatgcg agattcacgc ggcggattca
     6841 agcttgacga gtctccgtct cagacgcgcg cgccgtgtgc cggcgacggc gcacgggcaa
     6901 aaacctcgcg agcgaaaaga gcgcgcgaaa taagttgtct gggggcatgg cttgcggcat
     6961 gaagcgcccc atgctctatc tctatgcggg tttgatcgga ctgatcggcg gcgtgaccag
     7021 cgggctgttc ggcgtgggcg gcgggattgt gatggtgccc gccatgatgc tcctgctcca
     7081 gctcgatatc aaatccgcaa tcggcacctc cctggcggtg atcgtgccga cggcgctcat
     7141 cgggactttc aagcatcacg ggctggggca tgtcgactgg cgcctggcgt tggccctggc
     7201 gcccacggcc gtgctgggcg gattcgtcgg gtcctggctc acgaaattca tcgcctcggc
     7261 cgatctcaag cgcgccttcg gcggtttcct catccttgtc ggcgcccgac tcctcctgtt
     7321 caaatgaatg agccgagcgc tgaggcttgc caacgggcgt tcgcactatt gccgagtcgg
     7381 ggcgaaaccc gcgatgactt ttgttccgcc agtaacattg gacggacttg gtcatccggt
     7441 tcagcgtgtt gcttcggcag tagtgcgtga gcggcaaatc ggcggcggct acggttcgtg
     7501 gcagacaaga gacaaactca agcgccaggt ggctgcgccc gctcgggcag tgcccctggc
     7561 gccagtgtcg tttgtggaag tgcagccacg cgatccagac gcaagggctg acaagatcac
     7621 tggagcgcct aacagccgcc ccgcttcgcc gttcgagggt cgcgggcttc ggccagacgt
     7681 tccggttgtc gagcgtcgcg ggcgtcacca gggcatcacc ggcgcctcca cccagcacca
     7741 cccggctccg ttccccttgg agttggccac gcgcctgg
//
LOCUS       DAOQRO010000145         7776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9001_length_7776_cov_4.766222, whole genome shotgun sequence.
ACCESSION   DAOQRO010000145 DAOQRO010000000
VERSION     DAOQRO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9001_length_7776_cov_4.766222"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKK20_06885"
     CDS             <1..655
                     /locus_tag="PKK20_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNU99647.1"
                     /translation="LAKHVTSHDRPLPPAGFEARSPSLEAQVANLADEVAYSSHDLDD
                     GLEAGLLSEAALRREVRVWRQADARVRRAHGELPDECRRDFIIRCIIDDQVHDVVETT
                     ERRLRAARVESADAVRRRARPLVSYSPARRSSNLELRDYLYRNLYFNPAVRGPNTRAV
                     QMLEALFHHYLEHPHDLGEQSRKRVRTEGLHRAVCDYLAGMTDRYAIEEHARLIREQ"
     gene            673..2097
                     /locus_tag="PKK20_06890"
     CDS             673..2097
                     /locus_tag="PKK20_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNU99648.1"
                     /translation="MTNDLSLARLLGPEGTACAAAMEAGRRWLAGRDPAVLMAGVGRE
                     IAEVPAFAPIDKRALPASYGGDQPGPSDDFLVGQGLFYLSEQRRLFLDCTSGHYQMLW
                     GYNHPELEAAAFEAVRAGIVWDNHSNIPQAPLKQLGRRLIQAANDPDEPDPLDKVLLG
                     VCTGTVACAAALKTQIKVFERKTRTRAAPVILSLEGNYHGSDFMLQFMRGLWPLLPRP
                     CEFVNLEPNALDAIEAAFTRYGERIAGFWAEPVMMNREAILVPRATLELARRWCDRVG
                     ALLCLDEIQTGFWRPEIFDFRALGVRPDLVVLGKGMAAGFHPLSGVLFRSRHDVLEQY
                     DAISTNGSAALPALVALASMQLIHARAGQVEAAAAGVQSGFHALANEFPELVERAQGR
                     GHLMGLRLKSVEHAKALHRRLLADGIWTRVHAYHEGHRTVLTKLGLLADAQIVDFLLD
                     RIRQALRALSIPNSSTSRQTTNVP"
     gene            2094..3545
                     /locus_tag="PKK20_06895"
     CDS             2094..3545
                     /locus_tag="PKK20_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99649.1"
                     /translation="MTLRPRIGLLALTLELYETLAPGLRSSREAWVRRALVPPLAAAA
                     DVVFERAVFRRDDVECVMRQFEALGIDLVVVVFLTYAPSQIALPALVRTPLPILIWNT
                     QELWGVDSTFTTEVMIDNHGVHGTQDLANVLVRAGVSFHVVTSHASEPDGLAELRDFS
                     MAAAAVNSLRSARIGRLGHPFPGMGDLGVDTTHLAATLGCAWVNLPVDEYHQRAAAAP
                     ESDVRGLVACYREAYDVAPDVTSIELESAARAELALRGFVAEHRLSGLTYQFLAFGDD
                     ERTETLPFVAASRLMADGIGFGGEGDLIGAVGTLLLHRLNPPATFSEVFTIDFAGNAL
                     FMSHMGEANVAMARRDRPVPLVARTTPITRTRTRQLALVTSLEPGLATLAALALGPAQ
                     RWRLLAAPVAIEDYGPQPGFCVPHFKVRPQSDVRRFLTDYARAGGPHHNAVCFGDGRR
                     RLRFAAHILGAEYHEIGGDGARLDGDRPTDDIG"
     gene            3554..5062
                     /locus_tag="PKK20_06900"
     CDS             3554..5062
                     /locus_tag="PKK20_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNU99650.1"
                     /translation="MDASESRVLWLGLDLGTTNVKALVADRDGVVRGEAARPVALAAV
                     GDGGVEQDIEEIWAATVEAIRGALRGVDAAQVAAIGVSSQGGALQLLDAAGCPVGRVL
                     SWLDQRARQDDEALTAELGRPWFRRHVGHGGSGYAVGQILRLRREDPARLDPPNRVGF
                     VGDVIVERLCGRAAHDATSAGLTLLLNPARGDYDTDLLERLGLARGQLPDLISPREPA
                     GRLRREVAAATGLRPAIPVSAAVHDQYAAALGTGAVEPGVIMVGAGTAWVLLAVSSHL
                     PALVTDDAFVCSHVVSGLFGEILSLVNGGSVVSWALRLIGRGQAGPDQIETMLASAPP
                     GCDGLSCWPFVTPFGASGLEPGTRGRLAGIQLGHTPAHLLRAVVEGLTFELNRHLIFL
                     KQAGLPVDRLVLGGTVAGSRTTTQMMADVTGLALDCSAAGGGSVLGAAILARGLIEPD
                     RSLAELSQSMRAGPRRVEPGPDAVAYRELFHRYRASLPCSTAPRVRKIGHTA"
     gene            5059..6168
                     /locus_tag="PKK20_06905"
     CDS             5059..6168
                     /locus_tag="PKK20_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNU99651.1"
                     /translation="MKSTPLYHVWDYRDADRAFWRDEMEAWLPHKIIDAHTHVMDPRF
                     RLSPMTESMRRQYWVNEVFECIDADTAERCHRAVFPGRQVGCIAFGFPDLDFDLDGGN
                     TWLAEQCVRRGWHNLAVIRPQWSQAAIARLLDQPGVIGLKPYYALISENRETRDAHLE
                     SGIFDFLPHHVLEVANARGAWVTLHVPKAARLGHPDNLREIAELRRRYPNVILVIAHL
                     GRSYTEAHAREALPALADDPGIYCDTSAVLNPASHRIALECLGPRRVLYGSDNPICYM
                     RGRRQYDGRAYINRTSYPFHFNRTREAPEIEAQYTLFMYEDLRAIKQACVDLGLTASE
                     DIQAIFHDNAARLIDMVVRRKPAAERRIEEADASR"
     gene            6196..7353
                     /locus_tag="PKK20_06910"
     CDS             6196..7353
                     /locus_tag="PKK20_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99652.1"
                     /translation="MKTIRFGLIGCGLMGREFASAAARWCHLLDADARPELVAVCNRG
                     AGAFEWFRRHFPGIAQYTHDYRDLLANPAVDAVYVAVPHHLHCEVYCAAAAAEKHLMG
                     EKPFGIDRAANTAILDALERHPNVFARCASQWVFFPGAQRIGDLIERGAFGQVLEVNT
                     GFLHSGDLDPNRPINWKRRIEHNGEYGCMGDLGLHVCCLPFRAGWIPRNVRALLSDIV
                     RERPDGRGGMAPCRTWDNATLLCETLDSATGSLFPWTVKTQRIAPGHKNTWYIEVYGT
                     KASARFSTLDPKRLDLLEYAGGEQVWGAVQTGHETVFPTITGPNFEFGSPDAILQMWA
                     AFLHELVHRAPLRPFAGCATPADAALSHRLFTAALRSHARGSVEAVEPPAG"
     gene            complement(7361..7486)
                     /locus_tag="PKK20_06915"
     CDS             complement(7361..7486)
                     /locus_tag="PKK20_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99653.1"
                     /translation="MLSVRTRQISTTLYEHSPTHIGGREVCRARLTWEKTPAPKR"
     gene            complement(7480..7689)
                     /locus_tag="PKK20_06920"
     CDS             complement(7480..7689)
                     /locus_tag="PKK20_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99654.1"
                     /translation="MTTPASKPSVWTQRLKVCSYDADFTHRATSVSVCRYFLDAAWNH
                     ADFTGSMACASSRSITWASPGKATC"
BASE COUNT         1216 a         2560 c         2616 g         1384 t
ORIGIN      
        1 actggccaaa cacgtcacca gtcacgaccg cccgcttcct ccagccgggt tcgaggcgcg
       61 gtccccctcg ctcgaagctc aagtcgccaa cctcgccgac gaggtcgcct actcgagcca
      121 cgatctggat gacggactcg aagctggcct gctctccgaa gccgcactgc gccgcgaagt
      181 cagggtctgg cggcaggccg acgcccgggt ccgacgcgcg catggcgaac tgccggacga
      241 gtgccgacgc gacttcatca tccggtgcat catcgacgac caggtgcatg acgtggtcga
      301 gacgacggaa cgtcggctgc gggcggcacg ggtcgagagt gccgacgccg tccgccggcg
      361 cgcgcggcct ctggtgtctt acagccccgc ccgtcgatcc tcgaatctcg agctcaggga
      421 ctatctgtat cggaatctct atttcaaccc tgcggtccgc ggccccaaca cccgtgctgt
      481 gcaaatgctc gaagccttgt tccaccacta tctcgaacac ccgcacgacc tgggggagca
      541 atcgcgcaag cgggttcgga ccgaaggtct gcatcgggcc gtgtgcgatt atctcgcggg
      601 catgaccgac cggtacgcga tcgaggaaca tgcgcgtctg attcgcgagc agtgaattca
      661 cctctacacc acatgacaaa cgacttgagc ttggcgcgcc tgctggggcc cgaaggaact
      721 gcctgtgcag ccgcgatgga ggctgggcgc cgctggctgg ccggacgcga ccccgccgtg
      781 ttgatggcgg gcgtgggccg ggaaatcgcc gaggttcccg ctttcgcgcc gattgacaag
      841 cgggctctgc ccgcctcgta cggcggcgat cagcccggcc ccagcgacga tttcctggtt
      901 ggacagggcc tgttctatct gagcgaacaa cggcgactct ttctcgactg cacttcgggg
      961 cattatcaga tgctctgggg ctacaatcat cccgaactcg aggcggcggc gttcgaggcc
     1021 gtccgcgccg gtattgtctg ggacaaccac tcgaacattc cccaggcccc tctcaagcaa
     1081 ctgggccggc ggctcattca agccgcgaac gacccggacg aacccgatcc gctcgacaaa
     1141 gtgctcctgg gcgtgtgcac cggcacggtg gcttgcgccg ctgctctcaa aacgcagatc
     1201 aaggtgttcg agcgcaagac ccgcacccgg gcggcccccg tcatcctgtc gctcgaaggc
     1261 aattatcacg ggagcgattt catgctgcag ttcatgcgcg gtctgtggcc gctcctgccc
     1321 cgaccctgcg agtttgtgaa ccttgagccc aatgcgttgg atgcaatcga ggccgccttc
     1381 acacggtacg gcgagcgcat cgccggattt tgggccgagc cggtgatgat gaaccgcgag
     1441 gcgatccttg tcccgcgcgc gaccctcgag ttggcgcggc gctggtgcga tcgggtgggc
     1501 gctttgctct gcctcgacga aatccaaacc gggttctggc gccccgagat tttcgatttc
     1561 cgcgccctgg gcgtgcgtcc ggacctggtc gtgctcggca agggcatggc ggccgggttt
     1621 catcccctgt cgggcgtgct cttccgcagc cgccatgacg tgctcgaaca gtacgacgcc
     1681 atcagcacca acggctcggc ggccctgcct gcgctcgtcg cgctggcgtc gatgcagttg
     1741 atccatgccc gggccgggca agttgaagcg gcggcggcgg gggtccaaag cggcttccac
     1801 gccctggcaa acgagttccc ggaactggtc gagcgggccc agggccgggg gcacctgatg
     1861 gggcttcgcc tcaagtccgt cgaacatgcc aaggcgctgc atcgccggtt gctggcagac
     1921 ggcatctgga cgcgcgtcca cgcctaccac gaaggccatc gcacggtgct gaccaagctc
     1981 ggattgctgg cggatgcgca gatcgtggac ttcctgctcg accgaatccg gcaggccctc
     2041 cgcgcgcttt caatcccgaa ctcgagcacg tcccggcaaa ccacgaatgt cccatgacac
     2101 ttcgcccacg aatcggtctg ctggctctga cgctcgaatt gtacgaaacg ctcgctccgg
     2161 gcctgcggtc ctcgcgcgag gcctgggtcc gacgcgcact tgtgccgccg ctggccgccg
     2221 ccgccgacgt cgtattcgag cgggccgtgt ttcgacgcga cgatgtcgag tgcgtgatgc
     2281 gccagtttga ggctttgggg atcgacctgg tcgtggtcgt gttcctgacc tatgcgccca
     2341 gccagatcgc cctgccggca ttggtccgga cacccctgcc gatcctgatc tggaacacgc
     2401 aggaactgtg gggtgttgac agcacattca caacggaggt gatgatcgac aaccacggcg
     2461 tgcatggcac ccaggacctg gccaacgtgt tggtccgcgc cggcgtctcc ttccacgtcg
     2521 tcaccagcca cgcaagcgag ccggacggcc tcgccgaact gcgcgacttc agcatggccg
     2581 ccgccgcggt gaacagtctg cgctcggccc ggattggccg gttggggcac cccttccctg
     2641 gcatgggcga tctgggtgtg gacacgaccc atctggcggc gacgctgggc tgcgcctggg
     2701 tgaatctgcc ggtggatgag tatcaccaac gcgcggccgc ggcgcccgag tccgatgtgc
     2761 gcggccttgt ggcctgctac cgggaggcct atgacgtggc ccccgacgtg acctcgatcg
     2821 agctcgagtc cgcagcccgc gcagaactgg cgttgcgggg cttcgtcgcc gagcatcggc
     2881 tctcgggcct gacctaccag ttccttgcct tcggcgatga cgagcgcacc gagacgctgc
     2941 ctttcgtggc ggcgagccgc ctgatggccg acggcatcgg gttcgggggc gagggcgacc
     3001 tgatcggagc tgtggggact ctgttgcttc atcggttgaa tccgccggcc acattctccg
     3061 aggtgttcac catcgacttc gccggaaacg cgctgttcat gagccacatg ggagaggcca
     3121 atgtggcgat ggcccggcgc gatcgccccg tgccgctcgt ggcccggacg actccgatta
     3181 ctcggacccg cacccggcag ttggctctgg tcaccagcct cgaaccgggt ctcgccacgc
     3241 tggctgcgct tgcgctgggc ccggcccaac gctggcgcct gctggccgca ccggtcgcga
     3301 tcgaggacta cgggccgcag ccgggcttct gtgtgccgca tttcaaggtg cggccgcaat
     3361 cggatgtcag gcggttcctg accgactacg cgcgcgctgg cgggccgcat cacaacgccg
     3421 tctgttttgg cgatggccgc cgccgtctgc ggttcgccgc gcacatcctg ggagcggaat
     3481 accacgaaat cggcggggac ggcgcgcgcc tggatggcga tcgaccgacg gatgacatcg
     3541 gatgaggcga ggcatggacg cgagcgaatc gagagtgctt tggctggggc tggatcttgg
     3601 cacgacaaac gtcaaggccc ttgtcgcgga ccgcgacggc gttgtgcggg gcgaggcagc
     3661 gcgccccgtt gcactggccg cggtgggaga tggtggcgtc gaacaggaca tcgaggagat
     3721 ttgggcggcc acagtcgaag cgattcgcgg cgcgttgcgt ggagtcgatg cggcgcaggt
     3781 ggcggccatc ggcgtttcga gccagggcgg cgccctgcaa ctgctcgacg ccgccggctg
     3841 cccggtcggc cgagtgctca gttggctcga tcaacgcgcg cgccaagacg atgaggcgct
     3901 gaccgcggag ctggggcggc cctggttccg gcgccacgtc gggcacggcg gctccggcta
     3961 cgccgtcggc caaatcctgc ggcttcgtcg cgaggacccc gcccgactcg atccgcctaa
     4021 tcgcgtcggg ttcgtcggcg acgtcatcgt ggagcgactg tgcggacggg ccgcgcacga
     4081 cgccacctca gccgggctca cgctgctgct caatccggcc cggggcgatt acgacacgga
     4141 tctcctggaa cgactgggcc ttgcacgcgg ccagttgccc gatctgatct cgccgcgcga
     4201 gccggcgggc cggttgcgcc gcgaggttgc ggctgcgacc ggattgcgtc cggccattcc
     4261 cgtctccgcc gcggtgcacg atcaatatgc ggccgccctc ggcactgggg ccgtcgagcc
     4321 cggcgtgatc atggtcgggg ctgggacggc gtgggtcctg ctggccgtca gctcgcatct
     4381 gccggcgctg gtcaccgatg acgcctttgt atgcagccac gtcgtcagcg gcctgttcgg
     4441 agagattctc tccctggtca acggaggctc ggtcgtctcg tgggcgctcc gacttatcgg
     4501 gcgcggacag gccggaccgg accagatcga gaccatgctg gcgtcggcgc cgcccgggtg
     4561 tgatggtctg agttgttggc cgttcgtgac acctttcggc gcctccggcc tcgaaccggg
     4621 cacgcgcggt cggctggcgg gaatccagct cgggcacacg ccggcacacc tgctgcgagc
     4681 ggtcgtcgag ggactgacgt tcgagctgaa tcggcatttg atattcctga aacaagcggg
     4741 gctgcccgtg gaccgcttgg tcctgggtgg caccgtggcc ggcagccgca ccacgactca
     4801 gatgatggcc gatgtgacgg gcctggcgct cgattgctcg gctgcgggcg gcggcagcgt
     4861 cctgggcgcg gccatcctgg ccagaggcct catcgagccg gatcgctcgc tggccgagtt
     4921 gagccagtcc atgcgcgcgg ggccgcggcg ggtcgagccg ggacccgatg cggttgccta
     4981 ccgcgagttg tttcaccgct atcgcgcgtc cttgccatgc agcacggcgc cgcgcgtcag
     5041 gaagatcgga cacaccgcat gaaatcgaca ccgctctatc acgtttggga ctaccgtgat
     5101 gcggatcgcg ccttctggcg cgacgagatg gaggcgtggc ttccgcacaa gatcatcgat
     5161 gcccacacgc acgtgatgga tccgcgattc cggctcagcc ccatgaccga gtcgatgcgg
     5221 cgtcaatact gggtcaacga ggtcttcgag tgcatcgacg cggacacggc cgagcggtgt
     5281 catcgggcgg tgttcccagg ccgccaggtg ggatgcatcg cgttcgggtt tcccgacctc
     5341 gatttcgatc tcgatggggg caacacgtgg ctggcggaac aatgcgtccg gcggggctgg
     5401 cacaacctgg cggtcattcg tccgcagtgg agccaggcgg ccattgcgcg cctcctggat
     5461 cagccgggcg tgatcggttt gaaaccgtac tacgcgctca tcagcgaaaa ccgggagacg
     5521 cgcgacgcgc acctcgaatc gggcattttc gattttctgc cgcatcatgt cctcgaagtg
     5581 gcgaacgcgc gcggcgcgtg ggtgacgctc cacgtgccca aggcggcgcg cctggggcat
     5641 cccgacaatc tgcgcgaaat cgcggagctg cgacggcggt atccgaatgt gattcttgtg
     5701 attgcgcatc tggggcgttc ctacaccgag gcgcatgcgc gggaggccct tccggccctg
     5761 gcggacgacc cgggcatcta ctgcgacacg tcggcggtgc tcaatccggc ctcgcaccgg
     5821 attgccctcg agtgtctggg cccgaggcgg gttctttacg gatcggacaa tccgatctgc
     5881 tacatgcggg gccgacgcca gtatgacggg cgtgcttaca tcaatcggac cagctacccg
     5941 ttccatttca accggacccg cgaggcgccc gagatcgagg cgcagtacac ccttttcatg
     6001 tacgaagatt tgcgcgcaat caaacaggcc tgcgtcgacc tgggcctgac cgcctccgaa
     6061 gacatccagg ccatctttca cgacaatgcc gcgcggctga tcgacatggt cgtccggagg
     6121 aaaccggcgg ccgaacgccg gatcgaggag gccgacgcca gccgttgacc gccatttgcc
     6181 gcggctctct acgctatgaa aaccatccgt ttcggtttga ttggatgcgg gttgatgggg
     6241 cgtgaatttg ccagtgccgc ggcgcgctgg tgtcatctgc tggacgcgga cgcgcgcccg
     6301 gagcttgtcg ccgtatgtaa ccgcggggct ggggcgttcg agtggtttcg ccggcatttc
     6361 cccggcattg cccaatacac gcacgattac cgcgacctgc tggccaatcc agccgtcgac
     6421 gcggtgtatg tcgctgtgcc tcaccatctg cactgcgagg tgtactgcgc ggccgcggcg
     6481 gccgagaaac acctgatggg cgaaaaacct ttcgggatcg atcgggccgc caacacggcg
     6541 attctcgacg cgctcgaacg gcaccccaac gtgttcgcgc ggtgcgcctc gcagtgggtc
     6601 ttcttccccg gggcccagcg gatcggcgat ctgatcgagc gcggtgcatt cggtcaggtc
     6661 ctcgaagtga acacgggctt cctgcacagc ggggatttgg acccaaatcg tccgatcaac
     6721 tggaagcggc gcatcgagca caacggcgag tacgggtgca tgggcgactt gggattgcat
     6781 gtctgttgcc tccccttccg cgccggctgg attccccgca atgtccgtgc gctgttgtcg
     6841 gatattgtgc gcgagcggcc ggatggccgg ggcggaatgg cgccctgtcg gacctgggac
     6901 aacgcaaccc tgctgtgcga gacactcgac agcgccacgg gcagtctgtt tccatggacc
     6961 gtgaagaccc agcggatcgc gccgggccac aagaacacct ggtatatcga ggtctatggc
     7021 accaaggcat cagcccgctt ctcgacactc gatcccaagc ggctcgacct cctcgagtat
     7081 gcgggcggcg agcaggtctg gggggcggtc caaacagggc acgagaccgt cttcccgacg
     7141 atcactggcc ccaacttcga gttcggatcc cccgatgcca ttctgcagat gtgggccgcg
     7201 ttcctgcatg aactggttca tcgcgccccg ttgcgcccat tcgccggctg cgcaacaccc
     7261 gccgacgcgg cgctgagcca tcggctattc accgccgccc tgcggtccca tgcgcgcggc
     7321 tcggtcgagg cggtggaacc gcccgcgggc tagcgatcgt tcaccgcttc ggcgccggtg
     7381 tcttctccca cgtcaggcgc gcccggcaga cctccctgcc cccgatgtgg gtcggcgaat
     7441 gttcgtagag cgtcgtgctg atctgccgtg tgcggacgct cagcatgtcg ccttgccggg
     7501 actcgcccag gtaattgacc tcgaggaggc gcaggccatg ctccccgtga aatccgcgtg
     7561 gttccaggcc gcatcgagga agtagcggca cacgctcacg ctggtggcgc ggtgggtgaa
     7621 gtccgcgtcg taggaacaga ctttgagtcg ctgcgtccag acggatggtt tggaggcagg
     7681 cgtggtcatg cggggttgaa gtggaacagt tgcgcggcgt gcggtgggac gtcgaggtag
     7741 agtccctggc gatggaggtc gtcgccgacc cgctcg
//
LOCUS       DAOQRO010000146         7774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9008_length_7774_cov_4.789999, whole genome shotgun sequence.
ACCESSION   DAOQRO010000146 DAOQRO010000000
VERSION     DAOQRO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9008_length_7774_cov_4.789999"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="PKK20_06925"
     CDS             complement(<1..1352)
                     /locus_tag="PKK20_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU99655.1"
                     /translation="MGLGNAPLTPLEQPVTELRGVGPERAALLARLGIRTVGDLLLHR
                     PRRYEDRRRLERIAGLKLNESATTQGRIVAQGVKYWKQRRKSLFECVLDDGTGRLHCR
                     WWNLPFMENYFKVGDEVLVYGKVRSLKPRAIDHPETETIEGGEDRSIHLHRIVPIYPL
                     TEGLTQRWLRALIWRALEACGNAFEEPWPDLNRGNLPTRAVAVRQLHFPDEMGDAEAA
                     RGRLALDELTDLQLAIQRRRRRLHELARALPCGGDNRLMKRFLRSLGFKLTESQTEVL
                     REIRRDLAGSHPMRRLLQGDVGSGKTVVAACAALMTLESGFNVALMAPTEILAEQHGC
                     VFARWFGPLGLGVALQTGSQKSFLTAPAGEIAVSLAGPGDAKDPSEPGVALAGPVVTV
                     GTHALVEAGYCPDALGLAIIDEQHKFGVAQRERLVRKGHYPHLRTKRSRCATPNLCCS
                     S"
     gene            complement(1380..2363)
                     /locus_tag="PKK20_06930"
     CDS             complement(1380..2363)
                     /locus_tag="PKK20_06930"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNU99656.1"
                     /translation="MKIAVLGVGAWGTALANHLAGGGNEITVWDHDTPNLALLAAEGR
                     NEKYLAGVDLPRDWRVEPDLDRAWPGSDLVLLAVPSRAFRAVSRRLASFKGVVLSVTK
                     GIEFDSGLTMSGVLAQTAPGARRAVLSGPTLAFEVARGVPTAAVVASGDPSVAERVQA
                     LFHRPSMRVYTSRDVVGVELGGALKNVIAVAAGVCDGLGFGENSKAALVTRAIVEIRR
                     LGVACGAQADTFAGLSGLGDLVVTCFSPLSRNRALGERLGRGERLDDILARSVSVAEG
                     YPTARAAWQQARRHGVSTPIIDEVYAMLYEDKRPDQAVRDITARGSKPEDW"
     gene            complement(2360..3007)
                     /gene="plsY"
                     /locus_tag="PKK20_06935"
     CDS             complement(2360..3007)
                     /gene="plsY"
                     /locus_tag="PKK20_06935"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNU99657.1"
                     /translation="MSIAWHVAVAVAGYLMGSVPTGYLVARSRDVDIRKAGSGNIGAT
                     NVLRVLGKPAGILVLAIDALKGALACWLLPAAAIALAGAEAAPEVEGLRIAAGLAAIL
                     GHNYTCWLRFKGGKGIATSAGVLLVWMPAALGLALLVWVLAFLVSGYVSLASVVAAFA
                     LPFIVWLTGGSATLIAVAAVLGALAIYKHRSNIQRLRAGTEHRFRRSRTNGAPPA"
     gene            3128..3853
                     /locus_tag="PKK20_06940"
     CDS             3128..3853
                     /locus_tag="PKK20_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99658.1"
                     /translation="MIEVEGLTKLYGDFAAVSDLSFVVRPGEVLGLVGPNGAGKTTTL
                     RCLAGVMPPTQGTVRIAGHDLATAPLAAKQALAYFPDEPRLFDYLTVRQHLDFTARLY
                     QVADAERVGEELLRELEIVDKADALPGELSRGMKQKLAIACGLLHAPQVAFFDEPLTG
                     LDPYGIRRMKDTILERAREGMALLISSHLLHLVDEICTHLMILKQGRRVAYGTIDEVR
                     RQFAESAPDASIEDVFFRATGGS"
     gene            3921..5702
                     /locus_tag="PKK20_06945"
     CDS             3921..5702
                     /locus_tag="PKK20_06945"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HNU99659.1"
                     /translation="MFTALVFLQTRLFWNRVRVQIRRLRQPKYLAGTIAGAAYVYFFL
                     IRGLLSNNRSTGTQSPQLAGLGPEWATLIEVGAALMLVTVAAAAWLFPTKRASLDFTE
                     AEIAFLFPAPISRTGLIHYRIARSQIPLLFGSFIMTLFSGRLLQGGQAWMHWMGWWLI
                     LATLELHRLGAAFTRTRLMDRGVTSARRRLLVLGVLALLAVAFWTWARLSLTAPTSED
                     LADLSRLAGYLEHTARSGPWFWLLLLPRCILRPMLASSAGSFALALPPAAGLVAVLYV
                     WVMRSQVAFEEATIEQARARAKALASLRSGHWQTAARVKRRGAPFHLAPLGCRPIALL
                     WKNLISMGMAFTPRFWVAMAAMAVVGTMFMKQFMPQSVGLKIVGLLPVALVPMLFLVV
                     PQILTMDLRQDLAMIDTLKTYPLRGWHLVLGEVLAPVAVLTAVQCLCLAWGVAFFPDF
                     EASHGPSLSERLAIGIALAIAAPGLNMISLLIHNAAILLFPGWARVGPGQARGFEAMG
                     RSILVMIAQITALGLALVLPAGAFAALFFIVGPLWASWTVVLPIGALAALVLMAFEAY
                     AALRMLGDWFERMDISTELRTLDTTAA"
     gene            5949..7196
                     /locus_tag="PKK20_06950"
     CDS             5949..7196
                     /locus_tag="PKK20_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNU99660.1"
                     /translation="MVVVPAKMGRLNRRALLTRLQHLGVASRADLAKSLGMSQPTAGK
                     IAEELLELGVLEEIDGAEPAPASSKGRVKLGRPGRLLRLNRTVPRFVALQLGVSETCV
                     STLCLGVSFSDNWQHRFKTPGSAELWAHRLKAIASRLPSAAYWGVLVSVPGIVDDLAG
                     RVLFSPNLHWTEKVDLCRLVQEVWAVPVVLVQEERALALGYQHLHPRARDFLLVDFGE
                     SVGASLVVSGQLYDNLLPICAELGHVPVLGNKRPCGCGALGCLETLVSTRGLLQSFAA
                     AHGGLVPTWEFMIETLAIRGVEPWLATTLDATSVVIAGALNVLGVRTVVVTGSLPDLP
                     TAVMAHLSSAIRRGSIYARFGQIDIETAPRHRTAGLVAAGIDRLVVPMSNRDPAARGV
                     ASSSACDRHPGHSTPKHSVAANS"
     gene            7237..>7774
                     /locus_tag="PKK20_06955"
     CDS             7237..>7774
                     /locus_tag="PKK20_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNU99661.1"
                     /translation="MSTQYTIGLDYGTNSVRALIVNTANGNELATAVWGYEHGNAGVI
                     LGRDPNLARQHPIDYIRGAEVTIKKALAEARRVARGFTPSQIVGIGVDTTGSTPLPVD
                     ANGQPLALQKKFAKNPDAMAWLWKDHTGVAEAAEITRLAASIRPQYLAKCGGTYSSEW
                     FFSKILRCLRTAPAVFDAA"
BASE COUNT         1360 a         2739 c         2313 g         1362 t
ORIGIN      
        1 gacgagcagc acaagttcgg cgtggcgcag cgggagcgtt tggtgcgcaa gtgagggtag
       61 tgccccttgc gcaccaaacg ctcccgctgc gccacgccga acttgtgctg ctcgtcgata
      121 atagccaggc caagagcgtc cggacaatag cccgcctcga ccagggcatg cgtgccgacc
      181 gtcacaaccg gaccggccaa cgcgacgcct ggttcggacg ggtctttggc gtcgcctggg
      241 ccggcgaggg aaacggcgat ctcgcctgct ggcgctgtca gaaatgactt ctgactcccg
      301 gtctgcaggg ccacacccag tcccaacggc ccgaaccacc gcgcgaaaac gcatccgtgc
      361 tgttccgcga gaatctcggt gggggccatc agcgccacat tgaacccgct ctcgagcgtc
      421 atcagcgccg cacaggccgc cacaacggtc tttcctgacc cgacgtcccc ctgcaacagg
      481 cgccgcatcg gatggctccc cgccaggtcc cgccgaatct cacgcaacac ttccgtctgc
      541 gactcggtga gcttgaatcc caacgaccta agaaaccgct tcatcaaacg gttgtctccg
      601 ccgcatggca gggcgcgcgc aagctcatga agccgtcgac ggcgcctttg gatggccagt
      661 tgcagatcgg tcaattcgtc caacgccagc cgcccacgcg cggcctcggc atcacccatc
      721 tcgtcgggaa aatggagctg ccgcacggcc acggcccgcg tcgggagatt cccccgattc
      781 aggtccggcc agggctcctc gaaggcgttg ccgcaggctt cgagcgcgcg ccaaatcaaa
      841 gcgcgcaacc agcgttgcgt cagcccctcg gtcagcggat agatcggcac gatccgatgc
      901 aggtgaatgg accgatcttc cccgccctcg atcgtctcgg tctccggatg atcgattgcg
      961 cgcggtttga gcgagcgcac cttgccgtac acgagaactt cgtctcccac cttgaagtag
     1021 ttctccatga acgggaggtt ccaccagcgg cagtgcagcc gcccggtgcc gtcatcgagc
     1081 acgcactcga agagcgactt ccgccgctgt ttccaatact ttactccttg cgcgacaatg
     1141 cgcccctgtg tggtggccga ttcgttcaac ttgagcccgg caatgcgctc gaggcgccga
     1201 cggtcttcat agcgccgagg ccggtgaagg agcaggtcgc ccaccgtccg gattcccagc
     1261 cgcgccagca gcgcggcccg ctccggcccg acgcctcgga gctcagtgac cggctgttca
     1321 agtggggtca gcggcgcgtt gcctaacccc atagcctcgg attcccgccg gtctgccaat
     1381 taccaatcct ccggtttcga gcctcgggcc gtgatgtccc gaaccgcctg gtcaggccgt
     1441 ttgtcctcgt aaagcatcgc gtacacctcg tcgatgatcg gcgtgctgac accgtgtcga
     1501 cgggcctgct gccaggccgc ccgggcggtt ggataacctt cggcgaccga aaccgaacgc
     1561 gccaaaatgt catcgagtct ctcccctcgc ccaaggcgtt cgccaagggc gcgattccga
     1621 ctcagcggcg aaaagcaggt caccacgaga tcgcccagtc cactcaagcc ggcgaacgtg
     1681 tcggcttgcg ctccgcaggc gacgcccaaa cgccgaatct cgacgatcgc gcgtgtcacc
     1741 agggccgcct tcgagttctc gccgaatccc agcccgtcgc acacgccggc cgcaaccgca
     1801 atcacgttct tcaatgcgcc ccccaactcg acgccgacca catcacggct ggtatagacc
     1861 cgcatgcttg ggcgatgaaa caacgcctgc acccgctcgg caaccgacgg atcgccgctg
     1921 gcaaccacgg ccgccgtcgg aacaccgcga gccacttcaa aggccagcgt gggaccggac
     1981 agcaccgcgc gccgggcccc gggtgcggtc tgggcaagca ctccgctcat ggtcaggccg
     2041 gagtcaaatt cgatcccttt ggtcacactc aacacgaccc ctttgaagga ggccagccgg
     2101 cggctcaccg cacggaaagc cctcgatggc acggccagca gcaccagatc gctgcccggc
     2161 catgcccgat cgagatccgg ttccacccgc caatcgcgcg gcaaatccac accagccaaa
     2221 tacttttcat tccggccttc ggccgcaagc agcgcgagat tgggcgtgtc gtggtcccac
     2281 acggtgatct cgttgccgcc gccggccaag tgattggcca gcgcggtgcc ccacgcgcca
     2341 acgccaagaa cagcgatctt catgcgggtg gagcgccgtt cgtgcgcgac cgcctgaagc
     2401 gatgctcggt gccggcacgc agacgctgga tattcgagcg gtgcttgtag atcgccaggg
     2461 cgccgagcac ggccgccacg gcgatcagtg tcgcgctgcc cccggtcagc cacacgatga
     2521 acggcagcgc gaacgcggcc acaaccgatg ccaacgagac gtagccggac acgagaaagg
     2581 ccagaaccca gaccagcaac gccaggccaa gagccgcagg catccagacc agcagcaccc
     2641 ccgccgatgt ggcaatcccc tttccgccct tgaaccgcag ccaacaggtg taattgtggc
     2701 cgagaatagc cgccaaaccg gccgcaatcc gcaggccctc aacctcaggc gcggcttcgg
     2761 cccccgccaa cgcaatcgct gcggccggaa gcagccaaca ggccagcgcg cctttgagcg
     2821 catcgatcgc cagaacaagg atgcccgccg gcttgcccag cacccgcagg acattcgtcg
     2881 ctccaatgtt cccgctgccc gccttgcgaa tgtccacatc ccgactgcgc gccacgaggt
     2941 aacccgtcgg aaccgacccc atcaggtacc cggcaacagc gacggccaca tgccatgcaa
     3001 tggacatggg tcgactctag tgcggcggca agacaaaggc actctctttc tgccccggct
     3061 gggtttgacg ggctttactc catccagcca agccgctagc tttcaccccc gtgacgaacc
     3121 actccctatg atcgaggtcg aaggtctcac gaaactgtat ggcgacttcg ccgcggtcag
     3181 cgacctatcc tttgtggtgc gcccaggcga ggtgttgggg ttggtcggtc cgaacggagc
     3241 cggtaaaaca acgaccctcc gctgcctggc cggcgtcatg cctcccaccc aaggcacggt
     3301 ccggatcgcc gggcacgacc tggcgaccgc cccgctcgcg gcgaaacagg cccttgccta
     3361 tttcccggat gaaccccgcc tgttcgatta tctcacagtc cgccagcatc tcgatttcac
     3421 ggccaggctc taccaggtgg ccgacgccga gcgcgtgggc gaagaacttc tccgcgaact
     3481 cgagatcgtc gacaaggccg acgccctgcc tggcgaattg tcgcgcggca tgaaacagaa
     3541 actcgcgatc gcctgcggcc tgctccatgc cccccaagtc gcgttcttcg acgaaccgct
     3601 caccggcctg gacccgtacg gcatccgccg catgaaagac acgatcctcg aacgcgcccg
     3661 cgagggaatg gcgctcttga tcagttcgca cctgctccac ctcgttgacg agatttgcac
     3721 gcatctcatg atcctcaaac agggcagacg cgtggcctac ggaacaatcg acgaggtccg
     3781 ccgccagttc gccgagtcgg ctcccgatgc cagcatcgaa gatgtgttct ttcgggcgac
     3841 tggaggcagt tgaggacaag gcccatcgga cggcgaatcg taacaatggg gagtcaggcg
     3901 gcagaggcgg tgattcaagt atgttcacag cactcgtctt tcttcagacg cgcctgttct
     3961 ggaatcgggt ccgggtccag attcgcaggc tgcgccagcc caagtacctg gccggcacca
     4021 tcgccggagc tgcctacgtc tacttcttcc tcatccgcgg attgctctcg aacaaccgat
     4081 cgacgggaac gcagagtccg caactcgccg gtcttgggcc cgagtgggcc acgctgatcg
     4141 aggtcggcgc ggccctgatg ttggtcaccg tggcggcggc ggcgtggctg tttccgacga
     4201 agcgagcctc cctggacttc accgaggccg aaatcgcctt tctgttcccg gcgcccatca
     4261 gccgcaccgg gctcatacac taccgcatcg cgcgatccca gattccgctg ctcttcggat
     4321 cgttcatcat gacgctcttc tccggacggc tgcttcaggg aggtcaggct tggatgcact
     4381 ggatgggctg gtggctcatc ctcgcgactc tcgaactcca tcggctcggc gccgccttta
     4441 cgcggacgcg cctgatggat aggggcgtca cttcggcgcg acgccgcctg ctggtgctcg
     4501 gcgtgctggc cctgctggcg gtcgcattct ggacctgggc caggctgagc ctcaccgcgc
     4561 cgacctccga ggatttggcc gatctgtccc gattggccgg ctacctcgaa cacaccgccc
     4621 gaagcggacc gtggttctgg ctgttgctcc tcccgcgctg catcctccgc ccgatgctcg
     4681 cgagttcagc cggcagcttt gcgcttgctc ttccgcccgc ggcgggattg gtcgcggtcc
     4741 tctacgtctg ggtcatgcga tcccaagtcg ccttcgagga agcgaccatc gaacaggccc
     4801 gggccagggc aaaagccctg gcgtcactcc ggtcagggca ctggcagacc gcggcccgtg
     4861 tgaagaggcg aggggcgccg ttccacctgg cgcctctggg ctgccgccca attgctctgc
     4921 tctggaagaa cctgatctcg atgggaatgg cgttcacgcc ccggttctgg gtggcaatgg
     4981 cggcgatggc ggtggttgga acgatgttca tgaaacagtt catgccccag tccgtcggcc
     5041 tgaagatcgt cggcttgctc ccggtggcgc tggtcccaat gctgttcctg gtcgtgcccc
     5101 agatactgac gatggacctc cggcaggacc tcgccatgat cgatacgctc aagacttacc
     5161 cgctgcgcgg ctggcacctg gtgctcggcg aggtccttgc cccggtcgcc gtgctcaccg
     5221 ccgtccagtg cctctgcctg gcctggggcg tggcgttctt ccccgacttc gaggcgagcc
     5281 acgggccatc gctctcagag cgacttgcca tcgggattgc actcgccatc gcggcccctg
     5341 gcctcaacat gatctcgctc ctgatccaca acgccgccat cctcctgttc cctggctggg
     5401 cgcgggtcgg ccccggacag gcccgcggat tcgaggcgat gggacgcagc atcctcgtga
     5461 tgatcgccca aatcacggct ctgggactgg ccctggtgtt gccagccggc gcattcgccg
     5521 cgttgttctt tatcgtcggg ccgctttggg cttcctggac cgtcgtgttg cccatcggcg
     5581 ccctggcggc cctggtcctc atggcgttcg aggcctacgc cgcgctgcga atgctcggcg
     5641 actggttcga acggatggac atctcgaccg aactccggac tctcgacacg acagccgcat
     5701 aaagccgcgc cccaacaagg catcgagcgt aagaccgagt cacctcaccg ccctgagtgg
     5761 tcggcccaac cgacgaagtt gcgaagcagc cgctgcccaa tctcctggct cttccaaccc
     5821 gcacagcagt ccccttgcga agtctgacca tctgtgcacc gacggggtgt gaaacatggg
     5881 ggttggggcc attccgcttg acaaaccgag cgcgctcgct ttcccctttc gtaactttat
     5941 taagtattgt ggttgtcgtt ccggccaaga tgggtcgtct gaaccgtcgc gcgttgttga
     6001 cgcgactgca gcatcttggt gtggcgtccc gggccgatct cgccaagtcg ctcggcatga
     6061 gccaacccac cgcgggaaaa atcgccgagg aattactcga gttgggtgtg ctcgaggaga
     6121 tcgatggagc ggagccggca cccgcttcgt ccaaaggccg ggtcaagctc gggcgtcccg
     6181 gtcggctcct gcgactcaac cgcacggtgc cgcgcttcgt ggcgcttcaa ctgggcgtgt
     6241 ccgaaacatg cgtcagcacc ctctgcctgg gcgtgagctt ttcggacaat tggcagcacc
     6301 ggttcaagac ccccggctcg gcggagctct gggcccaccg cctcaaagca attgcctcgc
     6361 gcctgccctc ggccgcctac tggggcgttc tggtcagcgt gccggggatc gtggacgacc
     6421 tggctggccg agtgctcttc tcgcccaatc tgcattggac ggagaaggtc gacttatgcc
     6481 gcctggtgca ggaggtctgg gccgttcccg tcgtcctcgt ccaggaggag cgagccctgg
     6541 ccctgggata ccagcacttg cacccgcggg cccgggactt tctcctcgtg gatttcggtg
     6601 aaagcgtggg cgcatcgctg gtcgtcagcg gacagctcta cgacaatctt ctccccatct
     6661 gcgccgaact tggccatgtc cccgtgctgg gcaataaacg accgtgcggt tgcggagccc
     6721 tgggatgcct ggaaacgctc gtgtcgaccc gcgggctgct ccagagcttc gccgccgccc
     6781 acggcggcct cgttccgacc tgggaattca tgatcgagac actggccatt cgcggcgtcg
     6841 agccctggtt ggccacgacg ctcgatgcga cctccgtggt catcgccggc gcgctcaatg
     6901 tcctcggtgt ccgcaccgtg gtcgtgaccg gcagcctgcc cgacctccca acagcggtca
     6961 tggcccacct gtcctccgcc atccgcaggg ggtccatcta cgcgcggttc ggccagatcg
     7021 atatcgagac agcgcctcgc caccgcacgg cgggtctcgt cgcggccggg atcgaccgtc
     7081 tcgtcgtacc gatgagcaac cgcgatccgg cggcccgcgg tgtcgcttcg tcgtccgcat
     7141 gcgaccgaca ccctggccat tcgacgccca aacactctgt cgccgcaaac tcatgaacct
     7201 gtcttcaatc cactcccccg accctctcta cattccatga gcacccaata caccatcgga
     7261 ctggactatg gcaccaactc ggtgcgcgcc ctgatcgtca acaccgccaa tggcaacgaa
     7321 ctcgccactg ccgtctgggg ctacgaacac ggcaacgcag gggtcatcct cggacgcgat
     7381 ccgaatctgg cccgtcaaca cccgatcgac tacatcagag gcgctgaagt cacaatcaag
     7441 aaggctctgg ctgaggcccg gagagtggcg cgcggattca cccccagcca aatcgtcggc
     7501 atcggggtcg acacaaccgg cagcacgcct cttccagttg atgccaacgg ccagccgctg
     7561 gccttgcaga agaagttcgc gaagaacccg gacgcgatgg cctggctctg gaaggaccac
     7621 acgggcgtcg ccgaggccgc cgagatcacc cggctggctg ccagcatccg cccccagtac
     7681 ctggccaagt gcggcggcac ctactcgagc gaatggttct tcagcaagat tcttcgctgc
     7741 ctgcggaccg cccccgccgt gttcgatgca gcgc
//
LOCUS       DAOQRO010000147         7735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9067_length_7735_cov_4.853255, whole genome shotgun sequence.
ACCESSION   DAOQRO010000147 DAOQRO010000000
VERSION     DAOQRO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_7735_cov_4.853255"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKK20_06960"
     CDS             <1..711
                     /locus_tag="PKK20_06960"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNU99662.1"
                     /translation="ASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGANRVL
                     TMDLHAQQIQGFFDIPVDHLYAAPVMYQYLKQMQVGDLVVVSPDVGGLKMAYAYSQVL
                     SAGLAIVAKRRKSATEVESLTVIGEIRGKNVLMVDDLTETAGTLTTAAALLKRKGAKR
                     ILACVSHVLLNDLGVERLRKSSIDELITTDTVLRPVIDGVKITTLSVAGLLGEAIKRI
                     HTNSSVTSLFEFKGGRTS"
     gene            746..1510
                     /locus_tag="PKK20_06965"
     CDS             746..1510
                     /locus_tag="PKK20_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNU99663.1"
                     /translation="MKAVPLTAYPRTALRRHGVKKLRTAARVPAVIYGRKRAPQNLEI
                     TLKDLKDLIAESSARNVLLDLAVEGDAEPKRLALLQDVQQHPLSGGILHADFHEVSPD
                     EQVTITVPVETIGEAIGVKNSGGVLEHVLFQLKVRALPKDLPEVLTLDVSHLDLGQAI
                     HIGEIQPPPGVEILGNKNITVIAVSAPITEAEETAAAEAAEGAPLEPEVLSQKKEEEV
                     EGAEPAAGKGADKKAPEKASEKAGEKPADKKAEKKK"
     gene            1577..2182
                     /gene="pth"
                     /locus_tag="PKK20_06970"
     CDS             1577..2182
                     /gene="pth"
                     /locus_tag="PKK20_06970"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNU99664.1"
                     /translation="MENARLVVGLGNPGSDYARTRHNAGFLLAETLADRWQAVWRMDE
                     GLRVRLARSDREGLRCWLAEPMTYMNCSGTAVAAMMRYYRVSPEGLMVLVDDADLPLG
                     ELRMRPHGGAGGHHGLESVTEHLGTSDYARLRLGIGRQPEAGREIAAHVLGRFSRDEA
                     TLFAKVLERAADAVTCWLESGIVTAMNRFNGAVVAPATKES"
     gene            2183..2491
                     /locus_tag="PKK20_06975"
     CDS             2183..2491
                     /locus_tag="PKK20_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNU99665.1"
                     /translation="MKRYTGLFILDTAGKEEAIKDMIDRITADIAAAGGKIETVQKMD
                     KRPFARVADHRKTAGFYVNIIFEAAPPVVAALRTRFASDADVFRTLFTIAPAPQPVAG
                     "
     gene            2512..2982
                     /gene="ssb"
                     /locus_tag="PKK20_06980"
     CDS             2512..2982
                     /gene="ssb"
                     /locus_tag="PKK20_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNU99666.1"
                     /translation="MANFNKVILAGNLTRDPEVRYTSKGMAIAKLGLALNRTFTTESG
                     EKREEVTFVDVDAFGRQAEVIGQYMRKGRSILIEGRLKLDQWEDKTTHQKKSKLGVVL
                     ESFSFLDSGRGEGSHADPAAPRPAAAAPSSAAAPSAPVSADPDTASAEEDDVPF"
     gene            3052..3690
                     /gene="rplI"
                     /locus_tag="PKK20_06985"
     CDS             3052..3690
                     /gene="rplI"
                     /locus_tag="PKK20_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNU99667.1"
                     /translation="MAKTEVILTSNIVGLGAESDQVVVAAGYARNYLFPQRLAIPVSA
                     ANKRRLEVLRQRRAEREAHDLNSMTELAASLSRIVLKIAVKTGADGKMFGAITAGSIA
                     DELQRQCDVSLDKKKIHLEMPIRTLGEHEIELRLHADVHPKLRVQVESSTPVELPAIA
                     PAEAAKGAGPGAVARAGRPVRERKAGVEPKTPATKEAKPVRARPERVEKRKA"
     gene            3751..5019
                     /locus_tag="PKK20_06990"
     CDS             3751..5019
                     /locus_tag="PKK20_06990"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNU99668.1"
                     /translation="MPKLTINDVNLSGKRVFVRVDYNVPMEEKEGRMVVTDLTRVRET
                     LPTLEALIGQGARLVLAAHLGRPKGKREPSMSLAPVAIELGNLLGKPVRFVDDCVGEK
                     VQAAVAQLKAGDVLLLENVRYYAEEEANEPAFAARLAESAEAYVNDAFGSAHRAHAST
                     EGVARIVSQRGGLCVAGLLMERELKFLGDELESPARPFVVILGGAKVSDKIMVIDRLL
                     ERADTILVGGAMAYTFRLAQGRQTGRSLVEADKVEVAKAAATKAQARNVPFLLPVDDV
                     VAVPVKTDQLDKKGKPVIDYQNIRTNADLDCPSDAAGLDIGPATVKAYEEVIARAKTI
                     LWNGPMGLFEDKRFAAGTYAIAQAVARATGAGAKSIIGGGDSVKALNKAKLGDKVTFM
                     STGGGASLEFLEGRVLPGVAALTDRPAEAC"
     gene            5088..5852
                     /gene="tpiA"
                     /locus_tag="PKK20_06995"
     CDS             5088..5852
                     /gene="tpiA"
                     /locus_tag="PKK20_06995"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNU99669.1"
                     /translation="MQKDRKLIIAGNWKMNKTVAEALDLVGGLRRELASVKEVDLVVC
                     PPFTALGEVSKAILDSNVRLGAQNMSEHSGGAYTGEVSAMMLKEFSVRYVILGHSERR
                     QYYGESNALISRKALAAHAATLKPILCIGETLAEREAGQTEAVLESQLRGSLAGLSKE
                     HMEETVLAYEPVWAIGTGKTATTEQAQSAHAFVRHVVASMFDETIAKYVRIQYGGSVK
                     PSNARELMSQPDVDGALVGGASLEVRSFTDIVKNSI"
     gene            5873..6406
                     /gene="secG"
                     /locus_tag="PKK20_07000"
     CDS             5873..6406
                     /gene="secG"
                     /locus_tag="PKK20_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNU99670.1"
                     /translation="MALVIGLLTFLLVLNSLLIILLVLVQLPKKEAGAGLAFGGSASD
                     ALFGAGSGTVLSRITKYGVGIFLTLSFTVSVLYTHSAKVSRQSVMLELERQVGAASAI
                     PSAVATNVPAPVFQPLTTNLPLPVVPSTATNAPVAVPATAPSTNTPAAEADAAPAAAA
                     PPPAAANEPAAPAVPAP"
     gene            6435..>7735
                     /locus_tag="PKK20_07005"
     CDS             6435..>7735
                     /locus_tag="PKK20_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNU99671.1"
                     /translation="MGRRTRRLAGLACALVLCATGCRPSFQRADLVILNGAEPESLDP
                     HLVTGQADGRIVMALFEGLTRFDPRTAQPMPGLAERWDISDDGCVYTFHLRTNAAWST
                     GEPIVAEDVVWSWRRILSPETASDYVGVLFYLKNGEAFATGKLKDFDQVGVRALDART
                     LRVELESPAAFFLDLCCFQPFAVVPRQRIERHGDRWLHARPFMASGAYTLDSWRLNDR
                     VRLRKNPRYWDAANTQSAVIDLLSGVVPATALNLYLSGAVDIVWDKELVPPDLIDKLK
                     ERPDFHIAPSLGNYFIRVNVTRTPFDDPRVRRALTLVIDRDRIVSRITKGGERVAYCV
                     SPPGIANGTNVYEPPRGLRPDPDLARQLLAEAGYPHGRGFPTVTYTYNTQESHRKIGV
                     ELREMWGRELGLRVELRQLEWKAWLRAQSMLDYDLVRSSWVG"
BASE COUNT         1471 a         2389 c         2543 g         1332 t
ORIGIN      
        1 gccagccgga tcacggcggt gctcccgttc tacggatatg cccgacagga ccgcaaggat
       61 cagccgcggg tgccgataac ggcgaagctg gtggccaatc tgctggtagc ggccggggcc
      121 aatcgcgtgc tgacgatgga tctgcacgcg caacaaatcc aggggttttt cgatatcccg
      181 gtggatcatc tctacgcggc gccggtcatg taccaatacc taaagcagat gcaggttggc
      241 gatctggtgg tggtgagtcc ggacgtcggc ggcctgaaga tggcgtacgc ctattcgcag
      301 gtcttgagcg cgggtttggc cattgtcgcc aagcggcgca agagcgccac ggaggtcgag
      361 tcgctgaccg taatcggcga gattcgcggc aagaacgtgc tgatggtgga cgacctgacc
      421 gagacggccg ggacgctgac gacggcggcg gccctgctca agcgcaaggg ggccaaacgg
      481 attctggcct gcgtctcgca tgtcctgctc aacgatctgg gcgtcgagcg gcttcgaaaa
      541 tcctctattg acgaactgat cacgactgat acagtcctcc gccctgttat tgacggagtg
      601 aagataacca ccctgtcggt ggcgggcttg ttgggcgaag ctatcaagcg aatccacacg
      661 aattcctcgg taacttcgct tttcgagttt aagggcgggc gtaccagttg atgctgaccg
      721 tccgccgagt gacgaatgac ctgacatgaa agcagttcct ttgacggcct acccgcggac
      781 ggccctgcgg cggcatggag tgaagaaact ccggacggcg gcgcgcgtcc cggcggtgat
      841 ctacggacgc aagcgcgctc cgcagaacct ggagatcaca ctcaaggatc tcaaggacct
      901 gatcgccgaa tcctcggcga ggaacgtctt gctcgattta gcggtcgagg gcgacgccga
      961 gcccaagcgg ctggctttgc tgcaagacgt gcaacagcac ccgctctcag gcggcatcct
     1021 ccatgcggac ttccacgagg tgtccccgga cgagcaggtg acaatcaccg ttccggtcga
     1081 gaccatcggc gaggccatcg gggtgaagaa cagcgggggc gtgctcgaac acgttctctt
     1141 ccaactcaag gtccgcgcat tgcccaaaga cctccccgag gtgctcactc tcgatgtgtc
     1201 gcacctcgat ctgggccagg cgattcacat cggcgaaatc cagccgccgc ccggagtcga
     1261 gattctcggc aacaagaata tcacggtaat tgctgtgtcg gccccgatca ccgaggccga
     1321 ggaaacggcg gccgcggaag ccgccgaggg cgcgccgctc gaacccgagg tcctcagcca
     1381 gaagaaggaa gaggaagtcg agggggccga gccggcggct gggaagggtg ccgacaagaa
     1441 ggcgcccgag aaggcttcgg agaaagcggg tgagaagcct gccgataaga aggcggagaa
     1501 aaagaagtag ctgcggtgag acgcgcacgc gccagagggc gcgtgcccga tcaacccaac
     1561 ccggtcctgg acagagatgg agaatgcgcg tctcgttgtg ggtttgggga atccgggaag
     1621 cgactacgcc cgcacgcgtc acaatgccgg gttccttctg gcggagacac tggccgaccg
     1681 ttggcaggcg gtgtggcgga tggacgaggg tctgcgggtg cgtttggcgc ggtcggatcg
     1741 ggaaggactg cggtgctggc tggcggagcc gatgacctac atgaactgca gcggcacggc
     1801 ggttgcagcc atgatgcgat actaccgggt cagccccgag ggcttgatgg tgcttgtgga
     1861 tgacgccgat ttgccgctgg gtgaactgcg catgcgcccg catggcgggg ccggaggaca
     1921 ccacgggctc gagtcggtga ccgagcatct ggggacatcg gattacgcgc ggctgcggct
     1981 ggggatcggc cggcaacctg aggccggccg ggaaatcgcc gcgcacgtgc tcgggcgctt
     2041 cagccgggac gaggcgacgc tgttcgccaa agtgttggaa cgggcggcgg atgccgtgac
     2101 atgctggctc gaatcgggta ttgtcacggc catgaatcgt tttaatgggg cggtggtcgc
     2161 ccccgcaaca aaggaaagct gagtgaaacg ttacacaggt ttgttcatcc tcgacaccgc
     2221 tggcaaggaa gaggcgatca aggatatgat cgacaggatc acagccgaca tcgccgctgc
     2281 gggcggaaag atcgaaactg tccagaagat ggacaagcgt cctttcgcgc gcgtggcgga
     2341 tcaccggaag accgccgggt tctacgtgaa tattatcttc gaggccgccc cgccggttgt
     2401 cgcggcgctg cggacccggt tcgcctcgga cgcggatgtc tttcggacgc ttttcactat
     2461 cgctcccgca ccccaacccg tcgcgggctg aggtcgtttc acccacgatc tatggcaaat
     2521 ttcaataagg tcatcctggc cgggaacctg acgcgggatc ccgaggtccg gtacacgtcc
     2581 aaaggcatgg caatcgccaa gctcggattg gccctgaacc ggacgttcac caccgaatcg
     2641 ggcgagaagc gcgaggaggt cacattcgtc gacgtcgacg cttttgggcg acaggctgag
     2701 gtcatcggcc agtacatgcg caaaggacgc tcgattctga tcgaaggccg cctcaagctc
     2761 gatcaatggg aggacaagac cacacaccag aagaagtcaa agctcggcgt cgtcctcgag
     2821 tcgttctcgt tcctcgattc cggacggggc gaaggcagcc atgccgatcc ggcagccccg
     2881 cgcccggccg ccgccgcccc gagctctgcg gccgctccct ccgctcccgt ctcggccgac
     2941 ccggacacgg cgtcggcaga ggaagacgac gtgccgttct gaagcgccgc aatgattcag
     3001 cagccaagcg attctccccc aactgcgctc caaatcgatt ctcactgttt tatggcaaag
     3061 accgaagtca ttctgaccag caacatcgtg ggccttggcg ccgagtctga ccaggttgtg
     3121 gtcgccgccg gctacgcccg caattatctt ttcccgcaac gtctggcgat ccccgtgagt
     3181 gccgccaaca agcggcggtt ggaagtgctc cggcagcgcc gggccgaacg cgaggcgcac
     3241 gatctcaact cgatgaccga actcgccgct tcattgagcc gcatcgtgct gaagatcgcc
     3301 gtcaagaccg gcgcggacgg caagatgttt ggcgcaatca ccgcgggctc catcgccgac
     3361 gagttgcagc gtcaatgcga cgtgagcctc gacaagaaaa aaatccacct cgagatgccg
     3421 atccgcaccc tcggcgagca cgagatcgag ctgcgcctcc atgccgacgt ccaccccaaa
     3481 ctcagagtcc aggtcgaaag ctcgaccccg gtcgaactcc ccgcgatcgc cccggccgag
     3541 gcggccaaag gcgccggccc cggggcggta gctcgggcgg gacgtccggt gcgcgaacgc
     3601 aaggcgggcg tggaacctaa gacgcccgcg acgaaggaag ccaaacccgt tcgagcccgg
     3661 cctgagcgtg tcgagaagcg caaggcctga gtcgctccat ccggcatcct tgcgtcggtg
     3721 ccatgcgatc tataatctct gcacgttttt atgcctaagc tgacaatcaa cgacgtcaat
     3781 ctgagcggca aacgggtgtt cgttcgagtg gactacaacg tgccgatgga agaaaaggaa
     3841 ggccgcatgg tcgtcaccga cctgactcgt gtccgggaga ctctgccgac gctcgaggcg
     3901 ctgattggcc agggcgcccg gttggttctg gcggcccacc tgggacgtcc gaaaggcaaa
     3961 cgcgagccgt ccatgtctct ggcgccggtg gcgatcgagt tgggcaacct cctgggcaag
     4021 cctgtgcgtt tcgtcgacga ctgtgtgggc gaaaaggtcc aggcagccgt tgcgcaactg
     4081 aaggcggggg atgtgctcct gctcgaaaac gtccgttatt acgccgaaga ggaggcgaac
     4141 gagcctgctt tcgcagccag gctggccgag tccgcggagg cctacgtcaa cgatgccttc
     4201 ggatccgccc accgggcgca cgcctcgacc gaaggggtgg cgcgcatcgt gtcccagcgg
     4261 ggcgggctct gcgtcgcggg tctcctgatg gagcgcgaac tcaagttcct gggcgacgaa
     4321 ctcgaatcgc cggctcggcc ctttgtggtg atcctcggcg gagcgaaggt gtcggacaag
     4381 atcatggtga tcgaccggct cttggagagg gcggacacca ttttggtggg gggcgccatg
     4441 gcttacacgt tccgtttggc acagggccgc caaacggggc gctcgcttgt cgaagcggac
     4501 aaggtcgagg tggccaaggc ggcggccacg aaggcccaag cccgcaatgt cccgtttctg
     4561 ctgccggtgg acgacgtggt ggccgttccg gtcaagaccg accaactcga caagaagggc
     4621 aagccggtta tcgattacca gaacatcagg acgaatgccg atctggactg cccgtccgac
     4681 gctgccggac tggatatcgg gccggcgacc gtcaaggcct acgaggaggt catcgcgcgg
     4741 gccaagacca ttctctggaa cgggccgatg ggattgttcg aggacaagcg attcgcggcg
     4801 ggcacgtacg ccatcgcgca agccgtggct cgagccacgg gggctggggc caagagcatc
     4861 atcggcggcg gcgacagcgt gaaggcgctc aacaaggcca agctgggcga caaggtgact
     4921 ttcatgagca cgggcggcgg cgccagcctc gagttcctcg aaggccgcgt gctgcccggc
     4981 gtcgcggcat tgaccgacag acccgcggag gcatgctgac gcgtccggca gggaacacgc
     5041 accaaacacg caccatcgca agatcgacat tttaacctga tttggacatg caaaaagacc
     5101 gcaagctgat catcgccggc aattggaaga tgaacaagac agtcgccgag gccctcgatc
     5161 tcgtgggcgg gttgcgccga gaactggcat cggtgaagga ggtggacctg gtggtctgcc
     5221 cgcccttcac cgcgctgggc gaggtgtcga aggcgattct cgattcgaac gttcggttgg
     5281 gcgctcaaaa catgagcgag cactccggcg gggcgtatac tggggaggtc tccgccatga
     5341 tgctcaagga gttctcggtc cggtatgtga tcctgggcca ctcggagcgg cgacagtact
     5401 atggcgaatc gaacgcgctg atttccagga aggccttggc cgctcacgcc gcgacgctca
     5461 agccgattct ctgcatcgga gagacgctgg ccgaacgcga ggccggccag accgaggcgg
     5521 tcctcgagtc ccagcttcga ggaagcctgg ccgggctgag caaggaacac atggaggaga
     5581 cggttctggc gtacgagccg gtctgggcca tcgggacggg caagaccgcc accacggaac
     5641 aggcccagtc ggcccatgcc tttgtgcgcc acgtcgtggc ctcgatgttc gacgagacga
     5701 tcgccaaata cgtccgcatc cagtacgggg gcagcgtcaa accgtcgaac gcccgcgagc
     5761 tgatgagcca gccggatgtg gacggggcct tggtgggcgg ggcatccctc gaggtccgca
     5821 gtttcacgga catcgtcaag aactcgatct aagcggaagg acaccgatcg ttatggctct
     5881 ggttatcggc ctgctcacgt tcctgttggt tctcaacagt ctcctgatca tcctcctcgt
     5941 cctggtgcag ttgcccaaga aggaggcagg cgccggtttg gccttcggcg gcagcgcaag
     6001 cgacgccctc ttcggcgccg gctcggggac ggtcctcagc cgcattacca agtacggcgt
     6061 cggcatcttt ctgaccctct cctttaccgt gtcggtgctc tacactcatt cggccaaggt
     6121 cagccgacaa agtgtcatgc tcgaactcga acgccaggtc ggagcggcca gcgctatccc
     6181 gtccgcggtc gccacaaacg tcccggcccc tgtcttccag cccctgacaa cgaatctccc
     6241 attgccagtg gtgccgtcta cggcaacaaa cgcccctgtc gccgtccccg ctacggctcc
     6301 gtccacaaac acgcccgcgg cggaagccga cgcggcgccg gccgcagccg cgcctccgcc
     6361 cgccgccgcc aatgagccgg ccgcgcccgc cgttcctgca ccatagcctg actcgagcag
     6421 gtccagaggc ggccatgggc cgccggaccc ggaggctcgc cggcctggcg tgcgcattgg
     6481 tcttatgtgc aacggggtgc cggccttcat tccagcgcgc ggatctcgtc attctcaatg
     6541 gagccgagcc cgagtcgctc gatccccatc tggtcaccgg ccaagccgat ggacgcatag
     6601 tgatggccct cttcgagggc ctgacacgat tcgatccgcg cacggcccag cccatgccgg
     6661 gtctggccga gcgctgggac atttcggacg acggatgtgt ctatacgttc cacctgcgga
     6721 ccaatgcggc gtggtccacc ggcgagccga tcgttgcgga ggatgtcgtc tggtcgtggc
     6781 ggcggattct ctcgcccgag acggccagtg actacgtcgg ggtgttgttc tatctgaaga
     6841 acggcgaggc gttcgcgacc ggcaagctca aggacttcga ccaggtgggt gtccgggccc
     6901 tcgatgcccg gacgctgcgt gtcgaactcg aatcccccgc cgcgttcttc ctggaccttt
     6961 gctgtttcca gccgttcgcg gttgtgccgc ggcagcggat cgagcgccat ggcgatcgct
     7021 ggctgcacgc ccggccattc atggcgagcg gggcctacac gctcgattcc tggcggttga
     7081 acgaccgggt tcggctgcgc aagaatcctc gttactggga cgccgcgaac acccagagcg
     7141 cggtgatcga tctgctctcc ggcgtggtcc ccgcaaccgc tttgaatctc tacctctcgg
     7201 gcgcggtcga catcgtctgg gacaaggaac tcgtgcctcc ggacctgatc gacaagctga
     7261 aggagcgccc ggacttccac atcgcgcctt cactcggcaa ttacttcatt cgggtcaatg
     7321 tcacccggac acccttcgat gacccgcgtg tccgccgcgc ccttacgctg gtgatcgatc
     7381 gggaccggat tgtgtccagg atcaccaaag gaggcgagcg ggtggcgtat tgcgtgtctc
     7441 cgcccggcat cgccaacgga acgaacgtct atgaaccgcc tcgggggctg cgacccgatc
     7501 cggaccttgc ccggcaactg ctcgccgagg cggggtatcc tcatggacgc ggtttcccga
     7561 cagtgactta cacgtacaac acgcaggaga gccaccggaa gatcggggtg gaattgcgcg
     7621 agatgtgggg gcgcgagttg gggttgcgcg tcgagttgcg gcagttggaa tggaaggcct
     7681 ggctgagggc tcaatcgatg ctcgattatg atcttgtgcg aagcagttgg gtggg
//
LOCUS       DAOQRO010000148         7723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9087_length_7723_cov_3.883281, whole genome shotgun sequence.
ACCESSION   DAOQRO010000148 DAOQRO010000000
VERSION     DAOQRO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9087_length_7723_cov_3.883281"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1011
                     /locus_tag="PKK20_07010"
     CDS             79..1011
                     /locus_tag="PKK20_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family transcriptional regulator"
                     /protein_id="HNU99672.1"
                     /translation="MDCTKFIEEQVRNLRQRVGDGLAINALSGGVDSSVVTALGHRAL
                     GRQLKTIFVENGLMREGEAGRVIETFAALGIPVERVDARKEFLGALAGVTDPERKRCA
                     ITETFYKAVFGRVMRQSGANFLLHGTILTDIEETVAGIKRQHNILSQLGIDPERVYGY
                     QVLEPLETLRKDDVRELAGALGLPETIAQRIPFPGPALATRIVGEVTEERLATVRQAT
                     AIVEEELASTGAFQYLAVLLNDRATGIRDNRREYGQIIVVRCIESTDARTALVTEIEW
                     PALHRLAERITRIPGVNRCLYDLTPKPPATIEYV"
     gene            1049..1819
                     /locus_tag="PKK20_07015"
     CDS             1049..1819
                     /locus_tag="PKK20_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNU99673.1"
                     /translation="MQPSEEDIVIMPCLDMQNGRVVKGVHFVDIRDAGDPVACAQAYC
                     QAGADELAMLDITATVEGRRTLIDVVRRVAEAATVPLTIGGGIGDVASAAAVLEAGAD
                     KVSVSSAAFRKPDLIRRLMKEFGAKVTVSIDVDRNAELPSGYEVYIDGGRTATGADAV
                     EWARRVDGYGVPVILPTSKAGDGARTGYDLPVIRAMAQAVKARVVASGGAGALDHFAQ
                     AVEAGATILLAASVFHFGLIRIPQLKDHLRARGVRVRG"
     gene            complement(1923..2696)
                     /locus_tag="PKK20_07020"
     CDS             complement(1923..2696)
                     /locus_tag="PKK20_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNU99674.1"
                     /translation="MIVRVSTPTADEAASSALPYVLVNMAMTADGKIATANRRIASFG
                     SKRDLAHLYALRATVDAVMCGARTASAEDIHLGPGPARYRRRRIEQGLAEFNMRILVS
                     GHGRIAPASSIFTHRFSPILILTTELAGQKRLCQLDGPADEVVVCGRTRIDFRAALAW
                     LRSARGVKRLLCEGGGELNAALFSQGLVDELHLTICPFVFGGAAAPTIADGPGPRRLA
                     DAARFRLVSHRRIGDELFCVFRKSRPGRRLCRARPTAHL"
     gene            complement(2693..3838)
                     /gene="queA"
                     /locus_tag="PKK20_07025"
     CDS             complement(2693..3838)
                     /gene="queA"
                     /locus_tag="PKK20_07025"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNU99675.1"
                     /translation="MRTADFRYVLPPELIAQTPAIRREDARLLVLDRRAGRIEHRRFV
                     DLPDYLRPGDALVLNNSRVILARLRAEKAGGSARLEVLLAEENAPNDWWVLLRPGKRV
                     RPGTVLHFPPPAPDSPNRAAPSRLASLSTPSVVRAIVETKNAEGHYRLRFEGPANIKD
                     MLEALGEVPLPPYITRAGPPTLEDRERYQTVFAHPPGSTAAPTAGLHFTTRMLDALRR
                     RGIRVCHVTLHVGLGTFAPVKTERLEDHVMHAEVFDLPPATAETIQETRRAGHRVIAV
                     GTTSVRVLESVASPARSDAPESHAVLTLSPGPGRTRIFIRPPYAFRAVDALLTNFHLP
                     ESTLLMLVSAFAAPGETRGREMILRAYQEAIRERYRFYSYGDAMLLL"
     gene            complement(3845..5641)
                     /locus_tag="PKK20_07030"
     CDS             complement(3845..5641)
                     /locus_tag="PKK20_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNU99676.1"
                     /translation="MKTKNHPLRRPEAAPSASPPQTLKLESLCVGRRRHPRLDICLTL
                     GSVTEVSARAIVLGLFREVAPEGAASALDQRLGGAVSEFMARRMFGGNIGEIFVMPTG
                     RHPVRADLVMFAGLGTFDQFDEEVLELVTENVIRTLIRTDVEDLATVLLGGRSGPRVA
                     SLLEHMLKGFLRGLLDADDDRRFRRVTLCELDPQRFEEIKRELFRLAGTPLFGDVEVT
                     FDERILPRPPESLAPSILRGLSAPPGAASSYLIVRQEGQRAGRLKFRSSLLTAGAKAT
                     VITAERWTAQKELDALLARLDARDFTPADLPAFGQQLGTLLLADEVLAVLPSVRGQHL
                     AVVHDAPASRFPWETLRSGDWQPALDGGLSRRYLAENMSVARWLEERRTHPVLEVLLV
                     VNPTRDLIGAEAEGARIAALFGAHPSVRLTLREGAEARRSTLLEDFRSGRYDLLHYAG
                     HAAFDPVQPTRSGIVCAGGDVLSGADLSGLGNLPNLVVFNACESGRVRGRARRATGPD
                     GISQRVQRTVGLAEAFLRGGVANYVGTYWPVGDDPAKTFAASFYSAIANGQPVGNALI
                     AARMAVYTMREKTIDWADYIHYGNPHFVLKLR"
     gene            5844..7223
                     /locus_tag="PKK20_07035"
     CDS             5844..7223
                     /locus_tag="PKK20_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNU99677.1"
                     /translation="MNLAASIQRQRALFQTGATRPLAFRRARLRELEDAVGSHEQALL
                     EALHADLRKPPHEAFGSEIGLVRAELRHALRHLPSWMARRRRPVPWLAWPGRGFVEPE
                     PYGVALIIGPWNYPVQLLLAPLIGALAAGNCVVLKPSEFAPRTAAALARLIEAIYPED
                     YVTVVQGERETAEALLCERFDAIFFTGSTAVGRLVMAAAARHLTPVTLELGGKSPCIV
                     CADAPLETTARRIVWGKFLNAGQSCVAPDFVLVDRRVRDGLLAALQRAVRAFYGQDPQ
                     KSPDYARIVNPRHIDRLAGYLQGGKPIEGGRWDAQDLYFAPTILTDVAPDAPVMQDEI
                     FGPILPVLEFDRLENALEMLRERPTPLALYVFTRDRSMQERVLKGTRSGGVCVNDTLT
                     HMVGSGLPFGGLGESGLGAYHGRASFDCFSHSRSVLRRSLAIDPALRYPPPRISLAAL
                     KRASRFLLG"
     gene            complement(7411..>7723)
                     /locus_tag="PKK20_07040"
     CDS             complement(7411..>7723)
                     /locus_tag="PKK20_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99678.1"
                     /translation="VVPALGRAHFFALFSVIANLTLGIAPVLWGLFIDAFGQRRVSML
                     GIEWNRFSLFFLLVTLAYVAVFSLSRRLEEPRAGRVEDLLRDLLAQTPMRSWIRFWPR
                     G"
BASE COUNT         1322 a         2397 c         2701 g         1303 t
ORIGIN      
        1 gctgcgcgct cgaaatgcgg tcttatttcg tttcctgaag gctgaagagt tcaacgatgg
       61 tttagaatgg agatatctat ggactgtacg aagtttatcg aggagcaggt gcgtaacctg
      121 cggcaaagag tcggagacgg tctcgccatc aatgccctga gcggcggggt cgacagctcg
      181 gtggtgacgg ccctgggcca tcgggcgctc gggcgccagc tcaagaccat ctttgtcgag
      241 aacggattga tgcgcgaggg ggaggcgggg cgcgtgatcg agacctttgc ggcgctgggc
      301 atcccggtgg aacgggtcga tgcgcgcaag gagttcctgg gggcgttggc gggcgtgacc
      361 gatccggagc ggaagcgctg cgcgatcacg gagacgtttt acaaggccgt gtttggcagg
      421 gtgatgcggc aatcgggagc caactttctc ctgcacggca cgattctgac tgacatcgag
      481 gagacggtgg ccgggatcaa gcgtcagcac aacatcctga gccagctcgg gatcgatccg
      541 gagcgtgtgt atggatacca ggtgctcgaa ccgctcgaaa cactgcgcaa ggacgatgtg
      601 cgggaactgg cgggggcgct gggcttgcct gagaccatcg cccagagaat tccctttccc
      661 ggtcccgccc tggcgacccg aatcgtcggg gaggtgaccg aggaacggct tgccacggtc
      721 cgacaagcca cggccatcgt cgaggaggag ctggcgtcga ccggcgcgtt tcagtacttg
      781 gcggtgctgc tcaacgaccg cgccacgggg attcgcgaca accgccgcga gtatggccag
      841 atcattgtcg tgcgctgcat cgaatcgact gacgcgcgaa cggcgttggt gacggagatc
      901 gagtggcccg cgttgcatcg cctggcggag cgaatcaccc gaatcccggg cgtgaaccgg
      961 tgtctgtacg atctgacccc gaagccgccc gccacgatcg agtacgtgta gcgaggcgcg
     1021 cctgagccca atcccaatcc tcaaccttat gcaaccgtca gaggaggaca tcgtcatcat
     1081 gccctgcctg gacatgcaaa acgggcgggt cgtcaagggt gtgcatttcg tcgatattcg
     1141 cgacgcgggc gacccggtcg cctgcgcgca ggcctattgc caggcggggg ccgatgagtt
     1201 ggccatgctc gatatcaccg cgaccgtcga gggacggcgt acgctgatcg acgtggtgag
     1261 gcgcgtggcc gaggcggcga cggtgcccct gaccattggc ggcgggattg gggacgtcgc
     1321 gtccgcggcg gccgtgctcg aggccggggc cgacaaggtc tcggtgagtt cggcggcatt
     1381 tcgaaagccg gatttgattc ggcggttgat gaaggagttt ggggcgaagg tgacggtgtc
     1441 gatcgacgtg gaccgcaacg cggaactgcc ctcgggctac gaggtctaca tcgacggcgg
     1501 acgcacggcg acaggcgccg atgccgtcga gtgggccagg cgagtcgatg gctacggcgt
     1561 gcctgtcatc ctgccaacca gcaaagccgg cgacggcgcg cgcaccgggt acgacctgcc
     1621 ggtcattcgg gccatggccc aagcggtgaa ggctcgggtg gtcgcctcgg gcggggcggg
     1681 cgccctcgac catttcgcgc aggccgtcga ggccggcgca accatcctgc tcgcggcttc
     1741 cgtcttccac ttcggcctga tccggattcc acaactcaaa gatcacctcc gtgcgcgggg
     1801 cgtgcgggtc cggggctgac cgacacaaca gcgggacatc ttggcagatg atgccttcgg
     1861 tgacgcctcg ccaaagagcc aaatcccctc cagcagcatc ggaatggatt cccaccgaca
     1921 acctacagat gcgccgtcgg gcgtgcgcgg cacagccggc gtcctggccg gctcttccgg
     1981 aacacacaaa acagttcgtc gccgatgcgg cgatgcgaca ctagccggaa tcgagcggcg
     2041 tccgccagcc ggcgcggacc ggggccgtcg gcgatggttg gggccgcggc gcccccgaag
     2101 acgaatggac agatggtcag gtgcagttcg tcgacgagcc cctggctgaa caaggccgca
     2161 ttcagttcgc cgcctccttc gcacagcaaa cgcttcacgc cgcgggccga gcgcagccag
     2221 gccagggcgg cgcggaagtc gatccgggtt cgtccgcaga cgacgacctc atccgccggt
     2281 ccatcgagtt ggcagagtcg tttctgccct gccagttcgg tcgtcaggat gaggatggga
     2341 gagaatcggt gggtgaagat cgaactggcc ggagcgatcc gcccgtgtcc gctgaccagg
     2401 atgcgcatgt tgaactcggc cagtccctgc tcgattcggc gccggcggta acgggcgggg
     2461 ccgggcccga ggtgaatgtc ttcggcggac gccgttctgg cgccgcacat gacggcgtca
     2521 acggtggcgc ggagagcgta gagatgggcg agatcccgct tgcttccgaa ggaggcgatg
     2581 cggcggttgg cggtggcgat cttgccgtcg gcggtcattg ccatgttgac cagcacatat
     2641 gggagggcag aggaggctgc ctcatccgcg gttggtgtcg agacgcgcac aatcatagca
     2701 agagcatagc atcaccgtag ctataaaaac gataccgctc gcggatggcc tcctggtagg
     2761 ccctcaggat catctcacgg ccccgagtct cgcccggtgc ggcaaacgcg ctcacaagca
     2821 tcagcaaagt cgactctggc agatggaagt tcgtgaggag cgcgtcgacc gcgcgaaacg
     2881 cgtacggtgg gcggatgaag atgcgcgtgc ggccgggacc tggggacagg gtcagcaccg
     2941 catgcgattc gggcgcgtcg gatcgggcgg gcgacgctac gctttcgagg acgcgcaccg
     3001 acgttgtgcc gacggcgatg acgcgatggc cggcgcgccg cgtttcctga atcgtctctg
     3061 ccgtggctgg gggaaggtcg aagacctcgg catgcatgac atggtcctcc agacgttcgg
     3121 ttttgaccgg tgcgaacgtg ccaaggccga cgtgcagtgt gacatggcag acgcgtatgc
     3181 cgcgtcggcg caatgcatcg agcatccgag tggtgaagtg aagtccggcc gtcggggcgg
     3241 cggtcgagcc gggggggtgc gcgaagaccg tttggtagcg ttcgcggtcc tcgagggtcg
     3301 gggggcctgc gcgtgtgatg tagggcggga gcgggacctc tccaagggcc tcgagcatgt
     3361 ccttgatgtt cgcgggcccc tcgaaccgca accgataatg gccttcggca ttcttggtct
     3421 cgacgatcgc tcgaacgaca ctcggggtgg agagagacgc tagccgggac ggagcggcgc
     3481 ggttggggga gtcgggcgcg ggtggcggaa agtggaggac agtgcccggg cggacccgtt
     3541 ttccggggcg caagaggacc caccaatcgt tgggcgcatt ctcttcggcg agcagcactt
     3601 cgaggcgggc gctgccgccc gccttttcag ccctcaagcg ggctagaatg acgcgcgagt
     3661 tgttgaggac cagggcgtcc cccgggcgca agtaatcagg cagatcgacg aaccggcgat
     3721 gctcgatgcg cccggctcgc cggtcgagca caagaaggcg tgcgtcttca cggcggatcg
     3781 ccggggtctg cgcgatcaac tcaggtggca ggacgtaacg aaaatccgcg gtccgcatgg
     3841 cagatcaacg cagcttgagc acgaagtgcg gatttccgta gtggatgtaa tcggcccaat
     3901 cgatcgtttt ctcgcgcatg gtgtagaccg ccatgcgcgc ggcgatgagg gcgttgccga
     3961 ccggttggcc attggcgatc gcgctgtaga acgacgccgc aaacgtcttg gcggggtcgt
     4021 ccccgaccgg ccaataggtg cccacgtaat tggccacgcc gccccgcagg aaggcttccg
     4081 cgagtccgac cgtgcgttgc acccgttggc tgatcccgtc cgggccggtg gcgcgccgcg
     4141 cgcgtccacg cacccggccc gattcgcatg cgttgaagac gaccagattg ggcaggttgc
     4201 ccagcccgct caaatctgcg ccgctgagca cgtcgccacc cgcgcagaca atcccgctgc
     4261 gcgtgggctg caccggatcg aaggccgcat gcccggcgta atggagaagg tcgtagcgtc
     4321 cggacctgaa atcttcgagg agtgtcgagc ggcgggcctc ggcgccctcg cgcagggtaa
     4381 gccgcacgga cggatgcgcg ccgaacagcg cggcaatgcg cgccccctca gcctccgcgc
     4441 cgatcagatc ccgtgtggga ttgaccacaa gcaggacttc gagcaccggg tgtgtgcgcc
     4501 gttcttcgag ccagcgggcg accgacatgt tctcggccag ataacggcgg ctgaggcctc
     4561 cgtcgagcgc gggctgccag tcgccggagc gcagggtttc ccaggggaat cgggaggcgg
     4621 gggcgtcgtg gacgacggcc aggtgctgac cccggacgct gggcaacacg gcgaggactt
     4681 catcggcaag cagcagtgtg ccgagttgct ggccgaacgc cggcaaatcc gcgggggtga
     4741 agtcgcgcgc atcgagccga gccaacaggg cgtccagttc cttctgcgcc gtccagcgtt
     4801 ccgcggtgat gacggtggct ttggcgcccg cagtcaggag cgacgagcgg aacttgagcc
     4861 gtccggcgcg ctgcccttcc tgccggacga tgagatacga ggaggcggcg ccgggcggcg
     4921 cgctcaaccc gcgcagaatc gacggggcca ggctttcggg cgggcgcggg aggatgcgct
     4981 catcgaaggt gacttcgaca tcgccgaaca gcggcgtccc cgccagacgg aacaactcgc
     5041 gcttgatttc ctcgaaacgc tgcggatcga gttcgcacag ggtcacccgt ctgaaccggc
     5101 ggtcgtcgtc cgcgtccaag agcccccgca ggaatccttt gagcatgtgt tcgagcagcg
     5161 aggccacacg ggggcccgag cgcccgccca gcaacaccgt ggcgaggtcc tcgacgtcgg
     5221 tgcggattag cgttcggatc acgttctcgg tgaccagttc caacacctcc tcgtcgaact
     5281 ggtcgaatgt gcccaggccg gcgaacatca ccaggtccgc ccgcaccgga tggcgtccgg
     5341 ttggcatgac gaaaatctcg ccgatgttgc ctccgaacat gcgccgcgcc atgaactcgc
     5401 tcaccgcgcc gcccaaccgc tggtcaaggg ctgacgccgc cccttccggg gcaacctcgc
     5461 ggaagagccc caggacgatc gcgcgtgcgc tgacctcggt gacgctgccc agggtcagac
     5521 agatgtccag acgcgggtgt cggcgccgtc cgacacagag gctttcgagt ttgagcgttt
     5581 ggggcgggga tgcggagggc gccgcctccg ggcggcggag cgggtggttc ttggttttca
     5641 tgcggcgttc atgcaaggct cggcgtattc ggcgctgatc agccacggcg gaccgtcgtc
     5701 ctttcgcgcg aacatgggcg ccggtctggg gctggctcgt cgcgccgatc gggtggcgtg
     5761 cgggcgtttg acccgccgca accggccggg cacgcgtgct tgacgaagtg ataggcgaag
     5821 gcagagcttt ctccaagtcg attatgaacc ttgccgcctc catccaacgg cagcgtgccc
     5881 tcttccagac gggggcgacg cgtccgctcg catttcggcg ggcgcggttg cgcgaactcg
     5941 aggacgcggt cgggagccac gaacaggcgc tgctcgaggc gctgcatgca gacttgcgca
     6001 agccgccgca cgaggcgttc ggcagcgaga tcgggctggt gcgtgccgaa ctccggcatg
     6061 ctcttcggca tttgccttcc tggatggcgc ggcgccggcg tcccgtgccg tggctggcct
     6121 ggcctggccg cggcttcgtg gagcccgagc cgtatggggt cgccctcatc atcgggccat
     6181 ggaactatcc ggttcaactg ctgcttgctc ccttgatcgg ggccctggcg gcgggcaact
     6241 gcgttgtgct caaaccgtcc gaattcgccc cgcggaccgc cgccgctttg gctcgattga
     6301 tcgaagcgat ctatcccgag gactatgtga cggtcgttca gggggaacgc gagactgcgg
     6361 aggccctgct gtgtgagcgt ttcgatgcca tcttcttcac cggcagcacg gccgtgggcc
     6421 gtctggtgat ggccgcggcg gcgcggcacc tcacaccggt gaccctcgaa ctgggcggca
     6481 agtcgccctg catcgtgtgc gcggatgcgc cgctcgagac aacagcccgc cggatcgtct
     6541 ggggcaagtt cctcaacgcc gggcagtcgt gcgtagcgcc cgacttcgtg cttgtggacc
     6601 gtcgcgtccg cgatggtctg ctggccgcct tgcagcgggc ggtgcgcgcg ttctacggcc
     6661 aggatccgca gaagagcccc gattacgccc gcatcgtgaa tccgaggcac atcgaccggc
     6721 tggcgggcta cctgcaaggc ggaaaaccga tcgaaggggg gcgatgggat gcccaggatc
     6781 tctattttgc gccgaccatc ctgaccgatg tggcgccgga cgcgccggtg atgcaggatg
     6841 agatttttgg gccgattctg cccgtgctcg agtttgatcg gctcgaaaac gccctcgaaa
     6901 tgctgcgcga gcgcccgacc cctctggccc tttacgtctt cactcgagat cgctcaatgc
     6961 aggaacgcgt gctcaagggg acgcggtcgg gcggtgtctg cgtcaatgac acgctcaccc
     7021 acatggttgg gagcggcctg ccctttggag gactgggcga gagcggattg ggcgcctacc
     7081 acggacgggc cagcttcgat tgtttctcac actcccgctc agtcttgcgt cggtccctgg
     7141 caatcgatcc cgcactgcgc tacccgcccc cgcgaatctc gcttgcggcc ctcaagcgcg
     7201 cctcgcgatt cctgctcggc taacccgaat ttctcgtcac ttgaaggagt aatctaaaat
     7261 ccgggctgtg catttgtact tctcctgtgt gctccggctt cgcgttgtcc caaaatggtg
     7321 agaaatgcag gctaatgaga ttcgcggcgg gactcgaccc cgtggcgccg cgttgccaca
     7381 gcccgcgtgg tcccgggccg atggcggatt tcacccacgc ggccagaatc gaatccaact
     7441 gcgcatcggg gtctgagcga gcagatcgcg caagaggtct tcgacgcgcc cggcccgagg
     7501 ttcttcgagc cggcgcgaca gactgaacac ggcgacgtaa gcgagtgtca cgagcaggaa
     7561 gaacagactg aaccggttcc attcgatgcc cagcatcgac acccgccgct ggccgaaggc
     7621 gtcgatgaac agtccccaga gcacgggggc aatcccgagc gtgaggttgg cgatcacgga
     7681 gaacagggcg aagaagtgag cgcggcccaa ggccggcacc acc
//
LOCUS       DAOQRO010000149         7692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9144_length_7692_cov_4.818384, whole genome shotgun sequence.
ACCESSION   DAOQRO010000149 DAOQRO010000000
VERSION     DAOQRO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9144_length_7692_cov_4.818384"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PKK20_07045"
     CDS             <1..1294
                     /locus_tag="PKK20_07045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU99679.1"
                     /translation="PEMELTEETKQIGENLAALIDDGATIEVGIGRIPQALLGFLKDK
                     KDLGVHTEMVSDRIVELVQSGAVNGSRKTVDRGKIVASFCLGTRQLYEFIDNNPMFAF
                     HPTEYVNDPHVISQQNKMVAINVALEVDLTGQVCADSIGTRFFSGVGGQVDFNRGAAK
                     AHGGKAIIALPSTAKNGTISRIVTRLSPGAGVVTTRAGVNYVVTEYGVAYLHGKSIQE
                     RALALICIAHPNYRAQLLREAIEAKYLSASMSDKEGKIHVGPKELRTTHLLADGTQIN
                     FRPIHPTDEPRMRDLFYALSQQTMYYRFMSFRRVVPRRELQEFVYIDHRNDVTVVATL
                     PEAHGENIIAVGSYYLDPKHNMAEVAFVVHDDWQNRGIGTAMLRYLIHTARRNGIHGF
                     TAEVLTDNKAMQAVFNKSNCKIKTRFAGNVISFEMTFD"
     gene            1552..4185
                     /locus_tag="PKK20_07050"
     CDS             1552..4185
                     /locus_tag="PKK20_07050"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNU99680.1"
                     /translation="MRTWTVAAREVLEQYFGRVRPTLEASGADVAEVIDDLRRHLEQE
                     AAISQLEVVTEQDVRRLLARIGAPEPPASEASGRHEGRSSAVAATDSRPPGRLANAMT
                     IGFGIVLPTIALLIEWMSGMCAGAFFDPLPTLWHVALVALVPLTNWLLWRALTHGGEA
                     GRGWVGWAGGLAVGVELYYALIFLPLMLPGLLGIVFYGWGLLPLSPVLSLVVTLVLIR
                     RLRVQHTAIGSGAVRGVWPGFAVGLAGLVLLEIPEALTRYGLTLATDADPRTVQRGIN
                     LLRSWGDRGVLLRDCYGRTRAAENMDLVGWVVTGGRATRPEKAREIYYRVTGQPFNSV
                     AAPKVRTGRARWAGLDDWTWDFDQAGEQVGGRVKGLTLHSSRLDAVVEPDAAIAYCEW
                     TMEFKNGSARQREARAQILLPPGGVVSRLTLWVNGEEREAAFAGRGEVREAYEKIVRQ
                     RRDPVLVTTAGPDRVLVQCFPVPPDGGIMKVRLGITAPLEMKSAETGLMRWPHAIERN
                     FTIHETFRHQLWVEGPRPLASAGGALQTEQFKEGWHAIRGQAQETDLWLARNSVEVAR
                     EGTVREVWARDTRGATPSVVRRTLRAAAVRPPSRMVIVIDGSQSMDQMPVVAEVLTEL
                     PSTVEASVMIAGDEPISVGTVSADTRDRLARWRVRGGQDNVPALVEAWDLAAQKPDSL
                     IVWICAPVPEVLESAQGLRQRYERRPNGPSLMAVQTRVGPNRLLEALDGIAAVQLLPR
                     TGDLAADLRALFRSWTDSGSRWEWVQQREADASAAPREGAEASSHLVRLWAKDEIRRL
                     AVSRMTEEAVELAGRYQLVTPVSGAVVLETAQQYRSSGLEPVPPSTVPSIPEPSTWAL
                     FVLGTGMLLFVRRRRASRVSS"
     gene            complement(4396..5865)
                     /gene="lysS"
                     /locus_tag="PKK20_07055"
     CDS             complement(4396..5865)
                     /gene="lysS"
                     /locus_tag="PKK20_07055"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNU99681.1"
                     /translation="MEDTNTLIEQRRTKLAALRAQGLDPFRNKFAPSAGCGETRARFE
                     SGQLAEGARVAVAGRITSHRDMGKSQFLDLKDQSGRIQVYAQKQVLGETQYAIFKHLD
                     LADFIGVTGTMFRTKTGEPSVRVESFTILAKALRPPPAKWHGLEDTEIRYRQRYLDLM
                     SNDEVKRVFLLRSRILREIREFLHGRGFVEVETPMMQAIPGGAAARPFKTHHNALGCD
                     FYLRIAIELYLKRLLVGGIDRVFEIGRNFRNEGLSRKHNPEFTMLEAYQAFADYESMM
                     DLVQGLVCHVAQNVLGTLRIEHRNSAGDIARAIDLTPPWRRVRYKDIVRECAGADWFA
                     LAPDRRRVRAIELGAEIAPEYEDFEVTQAVFEKLIEPTLINPTFVTHAPKELIPLAKL
                     SPEDPTTVEVFECCINGQEIAPAYTEQNDPIEQRERLEHQAGGEQQKIDEDFLVALEH
                     GMPPAGGMGLGIDRLCMMLLGQESIRDVILFPQLKPKES"
     gene            complement(5889..6698)
                     /locus_tag="PKK20_07060"
     CDS             complement(5889..6698)
                     /locus_tag="PKK20_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNU99682.1"
                     /translation="MGVFYDTHAHLSSREFAGDLEDLLARAAAAGITRIVTVGIDLRS
                     SQRVVQIASDHPMVYAAVGWHPTHADDAPEDVRSDLRDLARHPKVVAIGETGLDYFHL
                     PSKRGGTAADDLRHTAKQKELFRQHLELAAEMGLNCVVHERKSFEDVLEAVRPFAGRV
                     RGQFHCFSGDVAALQRVLALGSIVSYTGILTFKNSGTVREAFAATPLQHFMLETDAPY
                     LAPEPYRGKRCEPAYVREIAAAAAAVKGISLEELSEATCAAAQAFFPRMGR"
     gene            7004..>7692
                     /locus_tag="PKK20_07065"
     CDS             7004..>7692
                     /locus_tag="PKK20_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU99683.1"
                     /translation="MKATLTRRRLLKLLTAAGLGAPFATRVVADEADASIQSAPSPEN
                     IVVPVSPRDRYPAVLSRGRVIQPRRPLAVMHETDVLVVGGGPAGFAAAVAAARAGSKT
                     ALIERYGYLGGLWTGGLVLLVYPTHATEKGVRTKVVRGVGDELLERIARYPDAATNHA
                     AGKPDPTTDPEITKLVMDQMVREADVTMIFHCWVADVVMDGNAVRGVVIESKAGRQAI
                     LSKVVVDASGDG"
BASE COUNT         1346 a         2312 c         2572 g         1462 t
ORIGIN      
        1 tccggagatg gagttgaccg aggagacgaa gcagatcggc gagaacctcg cggccctgat
       61 cgacgacggc gcgaccatcg aggtcggcat tggtcggatt ccgcaggccc tgcttggatt
      121 tctcaaggat aagaaggacc tgggtgtgca caccgagatg gtctccgacc ggatcgtcga
      181 actcgttcag tcgggcgcgg tcaacggctc gcgcaagacc gtcgatcgcg gcaagattgt
      241 ggccagcttc tgcttgggga cgcggcagtt gtacgagttc atcgacaaca acccgatgtt
      301 cgcctttcac ccaacggagt acgtgaatga tccccacgtg atcagccagc aaaacaagat
      361 ggtggccatc aacgtcgccc tcgaagtcga tctgaccggc caggtgtgtg cggattcgat
      421 cggcacccgg ttcttctcgg gtgtgggtgg ccaggttgat ttcaaccggg gcgcggccaa
      481 ggcccacggc ggaaaggcga ttatcgccct gccctcgacc gccaaaaacg gcaccatctc
      541 gcggatcgtc acgcgcctca gcccgggtgc cggggtcgtc acaacgcgcg ccggggtgaa
      601 ctatgttgtc accgagtacg gcgtcgctta tctgcacggc aagagcattc aggaacgtgc
      661 cctggccctg atctgcatcg cccatccgaa ctaccgcgcc cagttgctgc gggaggctat
      721 cgaggccaag tacctgtctg cctcgatgtc cgacaaggag ggcaagatcc acgtcggccc
      781 caaggaactg cgcacgaccc accttctggc cgatggcacc cagatcaact tccgaccgat
      841 ccacccgacc gatgaaccgc gaatgcgcga tctgttctac gccttgtcgc agcagacgat
      901 gtattaccgt ttcatgagtt ttcgccgcgt ggtcccgcgc agggagcttc aggagttcgt
      961 ctacatcgac catcgcaacg acgtgactgt cgtcgcgaca ttgcccgagg cgcacggcga
     1021 gaacatcatt gccgtcggca gttactacct ggatcccaaa cacaacatgg ccgaggtcgc
     1081 cttcgtcgtt cacgacgact ggcagaatcg cggcatcggc accgcgatgc tccgctacct
     1141 gatccacacg gcgcgccgca acggcattca cggctttacg gccgaagtgc tcacggacaa
     1201 caaggccatg caggccgtgt tcaacaagag caactgcaag atcaagacac gcttcgccgg
     1261 caacgtcatc agcttcgaga tgactttcga ttagccgaac ccggtccccg atccaacgcg
     1321 gcaggacaca agtccacccc atcgcatccg cccactcagc tctgaggggc ggttttcttt
     1381 tcgacacggt ctttgttctg cacagggttg gattgggcgc aaggaagcgg ctggagcccc
     1441 cagtccggtc gggatgaccg ctcgatccct acgcgcggtc cgacgaacca tgtatggcat
     1501 ggtggaagcg gcgggattga aaccacagga tggggctgag ggagcaatga catgaggacg
     1561 tggacagtcg cagcacggga ggtgctggaa caatacttcg gtcgcgtgcg gccgacactc
     1621 gaagcgtcgg gcgccgatgt ggccgaggtg atcgacgatc tgcgccgtca cctcgagcag
     1681 gaggccgcca tctcccaact cgaagtggtc accgaacagg acgtccgccg gttgctggcc
     1741 cggatcggcg cgccagaacc tccggcgtcc gaggcttcgg ggcgtcacga aggccggtcg
     1801 agcgcggttg cggctaccga ttcgaggcct ccgggccgtt tggccaacgc gatgacgatt
     1861 ggcttcggca ttgtgctacc gacgatcgct ctgctgatcg aatggatgag tggcatgtgc
     1921 gccggggcct tcttcgatcc gctcccgaca ctctggcacg tggcgttggt ggcgctggtt
     1981 cccctcacca actggctcct ctggcgcgct ctaacccacg gtggcgaagc cggtcgaggc
     2041 tgggtgggct gggcaggcgg cctggcggtc ggggtcgaac tctactatgc gctgatcttt
     2101 cttcctttga tgttgccggg cttgttgggc attgtcttct acggttgggg tctgctgcca
     2161 ctgtccccgg tgctctctct ggtcgtcacg ctcgtcctga tccggcgtct gcgggtccag
     2221 cacacagcta tcggctccgg cgccgttcgg ggcgtgtggc ctggattcgc tgtcggtctg
     2281 gcagggttgg tcctcctcga aatcccggaa gctctgaccc gctatgggct gacgctggcg
     2341 acggacgctg atccgagaac cgttcagcgc gggatcaatc tgctccgctc ctggggcgac
     2401 cgcggcgtcc tgctgcgcga ttgctacggg cgcacacggg cggcggagaa catggacctg
     2461 gtggggtggg tcgtcacggg cgggcgggcg acgcggcccg agaaggcgcg cgagatttac
     2521 taccgtgtga ccggtcagcc gttcaacagc gttgccgccc cgaaggttcg gactgggcgg
     2581 gcacgctggg ccggtctgga cgattggacg tgggacttcg accaggcggg cgagcaagtg
     2641 ggcgggcgag tcaagggcct cacgttgcac agttcacggc tggatgccgt tgtggaaccg
     2701 gatgcggcga tcgcgtattg cgaatggacg atggagttca agaacggtag cgcacgccag
     2761 cgcgaagccc gcgcacaaat cctgctcccg cccggcggtg tcgtctctcg cctgacactc
     2821 tgggtgaatg gagaggagag ggaggcggcg tttgcggggc gcggcgaggt cagagaggcg
     2881 tacgagaaga tcgtgcgcca gcggcgggat cctgtgctgg tcaccaccgc cggtccggac
     2941 cgagtcctgg tgcagtgctt tcccgtgccg ccagacggcg ggattatgaa agtgcgtttg
     3001 ggaatcacgg ctcctctcga aatgaagtcg gctgagacgg gtctcatgcg ttggccacac
     3061 gcgatcgaac ggaacttcac gatccatgag acgtttcgtc atcaattgtg ggtcgagggg
     3121 ccgcgacctc tggccagcgc gggcggcgcg cttcagaccg agcaattcaa ggagggctgg
     3181 catgcgatcc gggggcaggc ccaggaaacc gacctctggc tggccaggaa ctctgtcgag
     3241 gtcgcccgtg aaggcaccgt gcgcgaggtc tgggcccggg acacacgcgg cgccacgccg
     3301 tccgtcgtgc ggcgaacgct ccgcgcggct gcggtccgtc cgccctctcg catggtgatc
     3361 gtgattgacg gttcccagag catggatcag atgccagtcg tggcagaggt cctgacggaa
     3421 ctgccttcga ccgtcgaagc atcggtgatg atcgcgggcg acgaaccgat ttcggtcggg
     3481 acagtcagcg ccgacacgcg cgaccgattg gccagatgga gggtccgtgg aggtcaggac
     3541 aacgtgcctg cgctggtcga ggcttgggat ttggcagcgc agaagcccga cagtctgatt
     3601 gtctggattt gtgctccggt tccggaggtg ctcgagagtg cgcaggggtt gcgtcagcgc
     3661 tacgagcgca gacccaacgg cccgagtctg atggcagtgc agacccgagt gggtcccaat
     3721 cgtctgctcg aagcgctcga cgggattgcc gcagtccagt tgctgccccg cacaggagat
     3781 ctggctgccg atcttcgggc gctcttccgc tcctggacgg actcaggtag tcgatgggag
     3841 tgggtgcagc agcgagaggc tgacgcatcc gccgcgccac gggaaggggc cgaggcctcg
     3901 tcacacctgg tgcggctctg ggccaaggac gaaatccggc gtttggcggt gtcgcggatg
     3961 acggaggaag cagtcgagtt ggctggccgt tatcaactgg ttacgcccgt tagtggcgcc
     4021 gtcgtgctcg aaacggcaca gcagtaccga tcgagtgggc tcgagccggt gccgccatcc
     4081 actgtgccgt cgattccgga accatccacc tgggctctgt tcgttttggg cacggggatg
     4141 ctgctgtttg tgcgaaggcg tcgagcatca cgggtcagtt cgtgacgccg caagaacaga
     4201 atccgcaggc tgcagtgacg agcctgcggc ccgctgagtt tcgatgcagg gcggagcttc
     4261 gaatgaagcg atgcggtgac ccaatgatgc cgcgtgttgt ccacggatgg gcgcggcgtg
     4321 agccacatgg gacgaatggg atgccacaag gggacgggct atgtgttcag ttctccagcc
     4381 atcacccgtc acccatcaac tctctttggg cttgagttgg gggaagagga ttacgtcgcg
     4441 gatgctttcc tggcccagaa gcatcatgca gagccggtcg atgcccagcc ccatgcctcc
     4501 ggctggcggc atgccgtgtt cgagtgccac aaggaaatcc tcgtcgattt tctgttgctc
     4561 gccgcccgcc tggtgttcga gccgctcgcg ctgttcgatc gggtcatttt gctcggtgta
     4621 agcgggcgcg atttcctggc cgttgatgca gcactcgaaa acctcgaccg tcgtcgggtc
     4681 ctcgggcgaa agcttggcca gcggaatcaa ctccttcggc gcgtgggtga cgaaggtggg
     4741 gttgatgagc gtcggctcga tgagtttctc gaagacagcc tgggtcactt cgaagtcctc
     4801 gtattccggc gcaatttcgg cgcccaactc gatggcccgt acacggcgcc gatcgggggc
     4861 gagggcgaac caatcggctc cggcgcactc acggacgatg tctttgtatc ggacccggcg
     4921 ccagggcggg gtgagatcga tggccctggc gatgtcgccc gcggagttcc tgtgctcgat
     4981 gcgcagggtt ccgaggacat tctgcgcgac gtggcaaacg agtccttgaa ccaagtccat
     5041 catcgactcg tagtcggcga aagcctggta ggcttcgagc atggtgaact cggggttgtg
     5101 tttgcgggag agtccttcgt tgcggaagtt gcgcccgatc tcgaaaacgc ggtcgattcc
     5161 gcccacaagc aggcgtttga gatagagttc gatggcgatg cgcaggtaga aatcgcaacc
     5221 cagcgcgttg tgatgggttt tgaagggcct tgcggccgcg ccgccgggga tcgcctgcat
     5281 catgggcgtc tccacctcca caaagccgcg tccgtgcagg aactcgcgaa tctctctcag
     5341 gattcggctg cggagcagga acacgcgctt gacctcgtcg ttcgacatta gatcgagata
     5401 acgctgacgg tagcgaatct ccgtgtcttc gaggccgtgc catttggccg gaggcgggcg
     5461 gagcgccttg gccaggatgg tgaacgactc gacccgcacg ctcggttcgc cggtctttgt
     5521 gcggaacatc gtcccggtca ccccgataaa atcagccagg tcgagatgtt tgaagatggc
     5581 gtattgcgtc tcgcccagca cctgtttctg ggcgtagacc tggatgcgtc cgctttggtc
     5641 tttgagatcg aggaactggc tcttccccat gtcgcgatgg gacgtgatgc gaccggcaac
     5701 agcgacgcgc gcgccttcgg ccagttggcc ggactcgaac cgcgcgcgcg tctcgccgca
     5761 tccggcggag ggggcgaatt tgttccggaa cggatcgagg ccctgggccc gcaacgcagc
     5821 cagtttcgtc cgacgctgct cgatcaatgt gttggtatct tccatagcga ttccccgacg
     5881 gctcgatctc agcgccccat tcgaggaaag aacgcctgcg cggcggcaca ggtcgcctcg
     5941 ctcagttctt cgaggctgat gcccttgacc gcggctgcgg ccgcggcaat ctcgcggacg
     6001 tacgccggct cgcaacgctt gccgcgatac ggttcggggg ccagataggg cgcgtctgtt
     6061 tcgagcatga agtgttggag aggcgtggcg gcaaatgcct cccggactgt tcctgagttc
     6121 ttgaacgtca gaatacccgt gtagctgacg atcgacccaa gcgccaggac ccgctgcagc
     6181 gcggcgacat ccccggaaaa gcaatgaaac tgccctcgca cgcgccccgc gaacggccgc
     6241 acagcctcga ggacgtcctc gaacgacttg cgctcatgga caacgcaatt gagccccatc
     6301 tcggctgcca gttcgaggtg ctgacgaaac agctctttct gtttcgccgt atgccgcaaa
     6361 tcatccgccg cagtccctcc tcgcttggat ggcagatgga agtagtcgag ccccgtctcg
     6421 cctatggcca cgaccttcgg gtgccgggcc aggtccctca agtcgcttcg cacgtcctcc
     6481 ggcgcgtcat cggcgtgggt tgggtgccag ccgacggctg cgtacaccat tggatgatcg
     6541 ctggcgattt gcactacccg ctggctggac cgcagatcaa tgccgacggt gacgatgcgc
     6601 gtgatgccgg cggcggccgc tcgagccaga aggtcctcga gatccccggc aaactcgcgg
     6661 gaactcaagt gcgcgtgtgt gtcgtagaaa acgcccatgt atcgactcag cgcaaggcgg
     6721 accgggttcg tcctgagtgt cctctgccgg gctgcgtcgc gttcactggt ataccgtagg
     6781 aaatccgcga agtgatttca agctggaggg aatcgtggcc gtgcggctga gttgaccaac
     6841 gtgcgcatcg agggcgtcgc cggtggggtt gacttcaagg cttctattct ttgaaatggg
     6901 cctatgattc atcggacagg tcggttcatc cgcgcatcgg gtgcgttgtt gacatgcttc
     6961 ggccctttca catcgcggcg caggtcacgc aggcattgat tgtatgaaag ccacgctcac
     7021 ccggcgacgc ctgctcaagc tcctgacggc cgcggggctc ggcgccccgt tcgccacgcg
     7081 cgttgtggcc gacgaagcgg acgcgagcat ccaaagcgcc ccaagtcccg agaacatcgt
     7141 ggtgccagtc agcccccgcg atcgctatcc ggccgtgctg tcccgcggtc gtgtgatcca
     7201 gccgaggcgc ccgctggcgg tgatgcacga gacggacgtg ctggtcgttg gcggcggtcc
     7261 cgcgggcttt gcggcagcag tggcggccgc gcgagccgga tcgaagaccg cgctgatcga
     7321 gcgatacggc tacctgggcg gtctttggac gggtggtttg gtgctgctgg tctatccgac
     7381 gcacgcgacg gagaagggtg tgcggaccaa ggtggtgcgc ggtgtgggcg atgaattgtt
     7441 ggagcgcatc gcccggtatc ccgacgcggc tacgaatcat gccgcaggca agcccgaccc
     7501 aacaactgat ccggagatca ccaagctggt catggaccag atggtgcggg aggcggacgt
     7561 cacaatgatc tttcactgtt gggtggcgga cgtggtgatg gacggcaacg ccgtgcgcgg
     7621 cgttgtgatc gagagcaagg ccgggcgcca agccatcctt tccaaggtgg ttgtggatgc
     7681 ctcaggcgac gg
//
LOCUS       DAOQRO010000150         7622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9262_length_7622_cov_5.538126, whole genome shotgun sequence.
ACCESSION   DAOQRO010000150 DAOQRO010000000
VERSION     DAOQRO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9262_length_7622_cov_5.538126"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKK20_07070"
     CDS             <1..909
                     /locus_tag="PKK20_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU99684.1"
                     /translation="ARVFDILDTPEERMATGEPADQLARAGVSDHSSGLAAPSVPPSW
                     RARGEVMYDQGSFAYAEGKTALRNIALRARPGEMVALVGPTGAGKSTLVGLLPAFYQL
                     DSGRILIDGHDIRTLPLATLRAQIAVVTQEAFLFNGTLRENILYGRIEASEADLVAAA
                     RAANCHEFISRLPDGYESRIGERGVKLSVGEKQRVSIARALLMDAPILILDEATASVD
                     TATERLIQEALERLMQGRTSFVIAHRLSTIVNADQILVLRRGEIIERGRHDELVALGG
                     LYAKLARIQNTTFIEESFDRLADTTS"
     gene            1010..4489
                     /locus_tag="PKK20_07075"
     CDS             1010..4489
                     /locus_tag="PKK20_07075"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HNU99685.1"
                     /translation="MNTTVVRACPRPSTVLSMVPKARRGALRAALAASSQSPFLRRSS
                     RWTSVLAAAISLCVGMGWPGAAGEVQLTETRLVRPTYRVAPPDPNPRFYQGRTYQGAR
                     ATFYPYPVQDRLTEIKEDQSYRALVLENNCLQITVLPELGGRLFSAVDKSNGYDFFYR
                     QSVIKPALIGMLGAWISGGVEWNFPHHHRATSFMPVEVTTQTDADGSKTVWVGEIEWR
                     HRLKWIVGLSLHPDRSYIEMTMRLFNRTPFAHAFLFWINPAVHANEHYQVLFPPDAAF
                     AVQHGKPEFAHWPIARETYGGVDYSRGVDISWWRNHPSPISFFCFHSDQDFFGGYDHG
                     RRAGVLHYADHHVVPGKKFFEWGTGPEGELWSGILTDTDGPYLELMAGAFSDNQPDYS
                     WIQPGEVKSWKHYWYPIRELGGVKAATLDAALNLELTNRVARLSLNATAEFPNACARL
                     ELEDQTLFETNLTISPRQPFVMTRPIDAEVAPDQLRASLVAADGRVLVRWHPPTPRPD
                     DPPLPDPVRRPLPPKDIASIEDLYLTGLRIEQLYSPSFEPAPYYEEAIRRDPGDYRAN
                     TALGLLHAKAGRWDAAELHLRTAIERATANYIRPKDGEAQYYLGVVLKARGELDAAYD
                     AFQGAAWNHAWQAAAFHALAEIDAHRGRFAKALEHANDALARSGFNTKARGLKSALLR
                     RLSRADEAEETAQAALAFDPLDFLAAHELGLALRQRGRTDAADRAFAWLDATMPRQAQ
                     PWLELASDYAAAGLWTEVIDVLERHLKGNLDAADAHPLIFYHLAYAHEMAGRIQSLKL
                     YTHAARQSPDFCFPFRLEEERILRKALEWNPEDARAWYYLGNLLYDHRPEAAIAAWEK
                     SREIADAFPLVHRNLGLAYAQTRQDVDRAVSSLEQAVALNPKEPRFLYELDLQYEAAG
                     KPVAQRLAMLTNHHDTVVLRDDALTREIVLLTAAGRHDRAIELLTTRRFHNWEGRGEI
                     HTIYVDALLHRGHARLASGRAAQALEDYDAALLYPPNLEVGRPYRDRKTAQVHWYRGV
                     AYEALGNMAKAAECFALSVNSPESGPSEIRFAQALALAKLGRADDARRRFEDLVSAGQ
                     RQIEGGEQVDYFAKFGERESERVRKARAYYLMGLGQLGLGRRDDAKAAFAQALELHPA
                     HVGAALQQTALEN"
     gene            complement(4734..6239)
                     /locus_tag="PKK20_07080"
     CDS             complement(4734..6239)
                     /locus_tag="PKK20_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNU99686.1"
                     /translation="MSRPVAVRYHSGTVLNSSFTALDYIVVLVYLAAITAFGIFRGGR
                     QTSAQDYFLGREETPWWIVCFSIVAAETSALTFISIPGLAYLTNLTFLQVAIGYLIGR
                     IVVSYLLLPAYFRGELSTAYAFLEHRFGCKTRSFAAIVFLFTRIAADGVRLFATAIPL
                     KFVLDVNYPTAIVILAAVALAYTYFGGVRGVIWVDAVQMLVYVGGAATALVFLIVRAE
                     GGWSGISLAAFEAGKFQVIDWGLGAGFFQKPYTLIAGLVGGALLSMASHGTDQLIVQR
                     LLATRSLAAGRKALIGSGIIIVFQFALFLVVGLLLFGHYGAATVAELGLRRADEIFPK
                     YIIEGLPPGILGFIIAGLLAAALSTLAGSMSSLSSSTLLDLYRPLSGSTMDDARALWL
                     SRRITAGWAVLLVGSALFFMNTSQTVVELALSIASFTYGGLLGTFLLGVLLHKARQED
                     ALAAFVGGILVMVSVIGLKAVAWTWFTLVGVLATLLIGTLLSRLSRPDAMS"
     gene            complement(6292..7404)
                     /locus_tag="PKK20_07085"
     CDS             complement(6292..7404)
                     /locus_tag="PKK20_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU99687.1"
                     /translation="MYSTFTHLKRPCLARILALVLLFSNPINGLQLRAADPASPPTRN
                     YWGDTNRFLDQQAVVLLGAAQELLEQSPPALPEPAQRRTALLLLDAVLHDADAPKRAP
                     VQEFFQRRMAQAVLEIEQTRVERGAIIWKLYDHAFVVRTRTVTLAFDLTRAHSAGVAE
                     FAMPDALAARFASPCDALFISHLHGDHADDWVAQQFISQGKPVVVPAELWSDMPFHDK
                     LRHLSANSNQTHALPVQEGRQTLDVVVFPGHQGTAIVNHVFWVRTPEGLSFCHLGDQS
                     NTEDFVWIDCLADHGPLDILFPNCWTPDPLRVIRGAKPALVIPGHENELGHSIDHREP
                     YWLTYERFAGAAAPCVVMTWGEAFRYRIDPKTDESR"
BASE COUNT         1508 a         2632 c         2262 g         1220 t
ORIGIN      
        1 gcccgcgtct tcgacatcct cgacaccccc gaggagcgga tggcgaccgg cgaaccggcg
       61 gaccagctcg cccgcgccgg cgtcagcgat cattcctccg gcctcgccgc gccctcggtt
      121 cccccctcgt ggcgcgcgcg cggtgaggtg atgtacgatc agggcagttt cgcctacgcc
      181 gaaggcaaga ctgcgctgcg caacattgcg ttgcgcgccc ggcccggcga gatggttgcg
      241 ctggtgggcc caaccggcgc gggcaagtcg actctggtcg ggctgctccc ggccttctac
      301 caactggata gcggacgcat tctgatcgac ggccacgata tccggacgct gccgctggcg
      361 acactgcgcg cccagattgc tgtggtgacc caggaggcgt tcctgttcaa cggcaccctc
      421 cgcgagaaca tcctctacgg gcgcatcgag gcttccgagg ccgacctcgt cgccgccgcc
      481 cgcgccgcca actgtcacga gttcatcagc cgcctccccg acgggtacga gtcgcgcatc
      541 ggcgagcgcg gcgtgaaact gagtgttggc gagaagcagc gagtgagcat cgctcgcgcc
      601 ctgctgatgg acgcgccgat tctcatcctc gacgaggcga cggcaagcgt cgacaccgcc
      661 acggaacgcc tcatccagga ggccctcgaa cgtctcatgc agggacgcac cagcttcgtg
      721 atcgcccatc gcctcagcac catcgtcaac gcggatcaaa tcctcgtcct gcgccgcggc
      781 gagatcatcg agcgcgggcg ccacgacgaa cttgtggcct tggggggcct ctatgcgaag
      841 ctggcccgca tccagaacac gacgttcatc gaggagagct tcgaccgcct ggcggacaca
      901 acgtcttagc gtctgtgcaa aaatcacttc cgattagttt ttgcacggac cctaagagcg
      961 tcgattgtgc tcgtcttcct gaaggccggg cgcaaaatcg ccggcatcta tgaacaccac
     1021 ggtcgtccgc gcgtgccctc gtccctccac cgtcctttcc atggttccga aggcgcggcg
     1081 cggcgcactg cgcgccgcgc ttgcggcatc ctcccaatcc ccgttcctcc gccgctcgag
     1141 tcgatggaca tccgtgctcg cggcagccat ttcgctctgt gtgggcatgg gttggcccgg
     1201 cgcggccggt gaggtgcaat tgaccgagac ccgactggtc cgtccgacgt atcgggtggc
     1261 tccgcccgac ccgaatccgc gtttctacca gggccgcacc taccagggcg cgcgagccac
     1321 gttctatccc taccccgtcc aggaccgctt gaccgagatc aaggaggacc aatcctatcg
     1381 cgcactggtc ctcgagaaca actgccttca gatcactgtc ctgcccgaat tgggcggacg
     1441 cctcttcagc gccgtcgaca agagcaatgg atacgatttc ttctaccggc aatcggtcat
     1501 caagcccgcg ttgattggga tgctcggcgc ctggatttcg ggcggagtcg agtggaactt
     1561 cccgcatcac catcgcgcga cgagcttcat gccggtcgag gtcaccacgc agaccgacgc
     1621 cgacggcagc aagacagtct gggtgggcga aatcgagtgg cgccaccgtc tgaaatggat
     1681 cgtcggcctg tcattgcacc cggaccgctc ctacatcgaa atgacgatgc ggctcttcaa
     1741 ccgcacgcct ttcgcccatg cgttcctctt ctggatcaat cccgccgtcc acgccaacga
     1801 acactaccag gtcctgttcc cgcccgacgc cgctttcgcc gtgcagcatg gcaaacccga
     1861 gttcgcccac tggccaatcg cccgcgagac ctatggcggc gtagattact cccgcggcgt
     1921 cgacatcagt tggtggagaa accacccctc tcccatctcc ttcttctgct tccactcaga
     1981 ccaggatttc ttcggcggat acgaccacgg acgccgcgcg ggggtcctgc actacgccga
     2041 ccatcacgtg gtgccgggca agaagttctt cgagtggggg accggacccg agggtgaact
     2101 gtggagcggc atcctgaccg acaccgatgg cccctacctc gaacttatgg cgggtgcgtt
     2161 ctcggataac cagcccgatt acagttggat tcagcccggc gaagtcaaat cctggaagca
     2221 ctactggtat cccatacgcg agctgggcgg cgttaaggcc gccacactcg acgccgcgct
     2281 caacctcgaa ctcaccaacc gcgtcgcccg cctttcactc aacgcaaccg cggaattccc
     2341 caacgcctgc gcccggctcg aactcgagga ccagacctta ttcgaaacca acctcacgat
     2401 cagtccccgg cagccgttcg tcatgacacg gccgatcgat gccgaagtcg cccccgacca
     2461 actccgcgct tcactggtcg ccgcggatgg gcgcgtactg gtccgctggc atcccccgac
     2521 gccccgtccc gacgatccgc ccctgccgga ccccgtccgc cgtcctcttc cacccaagga
     2581 catcgcgtcc atcgaggacc tctacctcac gggattgcga atcgagcaac tctacagccc
     2641 gtcgttcgaa cccgcgcctt attacgagga agccatccga cgcgatcccg gcgactaccg
     2701 cgccaacacc gcgctcggcc tgctgcacgc caaggcgggc cgttgggatg ccgccgaact
     2761 ccacctccgc accgccatcg agcgcgcgac ggccaattac atccggccca aggacggcga
     2821 ggcccagtac tatctcgggg tcgtcctcaa ggcccgggga gaactcgacg cggcctatga
     2881 cgcgtttcag ggcgccgcct ggaaccacgc ctggcaggcg gcggccttcc acgccctcgc
     2941 cgagatcgac gcccaccgcg gccgattcgc caaggccctc gaacatgcca acgacgctct
     3001 ggccagaagc ggcttcaaca caaaagcccg cgggcttaaa tccgcgctgc tgcgacgcct
     3061 cagccgcgcc gatgaagccg aggaaaccgc tcaggccgcg ctcgccttcg atccgctcga
     3121 tttcctggcc gctcacgaac tcggcctggc cttgcgccag agagggcgga ccgacgccgc
     3181 cgaccgcgca ttcgcctggc tcgacgccac catgccccgg caagcgcagc cgtggctcga
     3241 attggccagc gactacgccg ccgccggtct ctggaccgag gtgatcgacg tgctcgaacg
     3301 ccacctcaag ggcaatctcg acgccgcgga cgcccatccc ctgatcttct atcacctcgc
     3361 ctacgcccac gaaatggccg gcagaatcca atccctcaag ctctacaccc acgccgcccg
     3421 gcaatccccc gacttctgct tcccgttccg cctcgaagag gaacgaatcc tcaggaaagc
     3481 gctcgaatgg aaccccgaag acgcccgggc ctggtattat ctgggcaatc tgctctacga
     3541 ccatcgaccc gaagccgcga tcgccgcctg ggaaaagtcg cgcgaaatcg ccgatgcctt
     3601 tccgctcgtc caccgcaacc tcggcctggc ctatgcgcaa acgcgccagg atgtggatcg
     3661 agccgtcagc agtctcgaac aagccgtcgc gctcaacccc aaagaaccgc gctttctcta
     3721 cgaactcgac ctgcaatacg aggcggccgg aaaaccggtc gcacagcggc tggccatgct
     3781 caccaatcat catgacaccg tcgtcctgcg cgacgacgcc ttgacccgcg agatcgttct
     3841 gctgaccgcg gccggccggc acgatcgcgc gatcgagttg ctcaccaccc gtcggttcca
     3901 caactgggaa ggccgcggcg aaatccacac catctacgtc gacgccctcc tccatcgcgg
     3961 ccacgcccgc ctggcatcgg gtcgcgccgc ccaggccctc gaggattacg acgcagccct
     4021 cctttacccc cccaaccttg aagtgggcag accctaccgc gaccgcaaaa cagcccaagt
     4081 ccactggtac agaggcgtcg cgtacgaagc cctcggcaac atggcaaagg ccgccgaatg
     4141 cttcgcactc tccgtcaaca gcccggaatc cggcccctcc gaaatccgat tcgcccaagc
     4201 gctcgccctg gcaaaactgg gccgcgccga cgatgccagg cggcgattcg aggatttggt
     4261 gagcgccgga caacgacaaa tcgagggcgg cgaacaggtc gattacttcg ccaaattcgg
     4321 cgaacgagaa tccgaacgcg tccgcaaagc gcgcgcctat tacctgatgg gcctggggca
     4381 gctcggcctc gggcgcaggg acgatgcgaa agccgctttt gcccaggcgc tcgaactgca
     4441 tccggcgcac gtcggtgccg cgcttcagca gaccgcgctg gaaaactgac ctccatctgg
     4501 cgcgcatttc tcgccaactt ctgaagaacg cggagccgat gtcatcgaaa ggtgcgctcg
     4561 tgcgcgctcg ccaggcgaaa ccgtgccgga atcgagcgag ggacgaaggc aacggagtgt
     4621 tggttcgcaa cggcgtttcc gtccacaacc agactctcat tgccgcctgt cccggcgcgg
     4681 ttttgccgca gcccggattt cagattactc ctccaagcgg tgagaaagcc gggttaggac
     4741 attgcgtccg gccggctcaa ccggctcagc agggtcccga tcagcagagt ggccaggacc
     4801 cccaccaagg tgaaccaggt ccacgcaacc gccttgagac cgatcacgga gaccatcacg
     4861 agaatgcctc caacgaatgc cgccagggcg tcctcctgcc gcgccttgtg cagtagcaca
     4921 cccagcagga acgtgcccag aagcccgccg taggtgaacg aggcaatgct gagcgccagc
     4981 tcgacgaccg tctggctcgt gttcatgaag aacaacgccg agccgacgag caaaaccgcc
     5041 cagcctgccg tgatccgccg ggagagccaa agcgcgcgcg catcgtccat tgtgctgccg
     5101 ctcaggggac gatacagatc gagcagggtg gatgaggaca gggaactcat cgatcccgcg
     5161 agggtggaca gcgcggcagc gagaagcccc gcaatgataa accccaaaat gccgggcggc
     5221 agaccttcga tgatatactt cggaaaaatc tcgtcggcgc gtcgcagacc gagctccgcg
     5281 accgtggcgg cgccgtaatg gccgaacagc agcaggccaa cgaccaggaa cagcgcgaac
     5341 tgaaagacga tgataatccc gctgccgatc agggccttcc ggccggccgc caaggatcgc
     5401 gtggcgagga gccgttggac aatcagttga tcggtgccgt gggacgccat cgacagcagc
     5461 gcacccccca cgagtccggc gatgagcgtg tacggcttct ggaagaaccc ggcgcccaat
     5521 ccccaatcga tcacctggaa cttccccgcc tcgaaggcgg ccaggctgat cccgctccag
     5581 ccgccctcgg ctcggacgat gagaaacacc agcgcagtgg cggcgccgcc aacgtagacc
     5641 agcatctgca ccgcatcgac ccagatcacc ccgcgcacac cgccgaagta ggtgtaagcc
     5701 agagccaccg ccgccaggat gacgatggcc gtcgggtagt tgacatcgag cacgaatttg
     5761 agcggtatcg cggtggcaaa gagccgcacg ccatccgccg cgatccgcgt gaacaggaac
     5821 acgatggccg caaaactccg cgtcttgcac ccgaaccgat gctcgagaaa ggcatacgcg
     5881 gtcgaaagtt cgccgcggaa gtaggcgggc agcagaaggt agctcaccac aatccgccca
     5941 atcagatagc cgatcgccac ctgcagaaac gtcaggttcg tcagatacgc caggcccggg
     6001 atgctgatga acgtcagcgc gctggtctcg gccgccacaa tcgagaaaca gacgatccac
     6061 cagggagtct cctcgcgccc gagaaaatag tcctgcgccg aggtctggcg cccgcctcgg
     6121 aaaatgccga aggcggtgat cgcggccagg tacaccagca ccacgatgta atcgagggca
     6181 gtgaaactcg agttcagcac ggtcccgctg tgataccgga cagccacggg ccgtgacaag
     6241 cctgcttagg gtatgtgcaa agactactcc gatcaattct tgcacagacc ctcagcggga
     6301 ttcgtcggtt ttagggtcga tccgataacg aaacgcctcc ccccaagtca tgacaacgca
     6361 cggcgcagcg gctccggcaa aacgctcgta cgttagccaa tacggttcgc gatggtcaat
     6421 cgagtgcccg agttcgttct cgtgtccagg aatcaccagc gccggcttcg ccccgcggat
     6481 cacgcgcagc ggatcgggtg tccagcagtt ggggaacagg atatcgagcg ggccatgatc
     6541 ggccaaacaa tcgatccaga cgaaatcctc tgtgttggac tggtcgccca ggtggcaaaa
     6601 ggaaaggcct tcgggggtgc gcacccagaa cacatggttc acaatggccg tcccttgatg
     6661 cccaggaaac acaaccacat cgagggtctg tcggccttcc tggacaggta aggcgtgcgt
     6721 ctggttcgag ttcgcggaca ggtgccggag cttgtcatgg aagggcatgt ccgaccacag
     6781 ctcggcgggg acaacgaccg gcttgccttg gctgatgaac tgctgcgcca cccagtcgtc
     6841 ggcgtgatcg ccgtgcagat gactgatgaa gagcgcatcg caggggctcg caaaccgcgc
     6901 ggccagggcg tcgggcatgg cgaactccgc tacccccgcc gagtgcgccc gagtcaggtc
     6961 gaaggcgagg gtgaccgtcc gggtgcgaac cacaaaggcg tgatcgtaaa gcttccagat
     7021 gattgcaccg cgctcgacgc gtgtctgctc gatctccagg accgcctggg ccatgcggcg
     7081 ctggaagaac tcctgcaccg gggcgcgctt gggagcgtcc gcatcgtgga gcaccgcgtc
     7141 gagcaggagc agcgcggttc ggcgttgggc cggttccggg agggcgggcg gcgactgctc
     7201 cagaagctcc tgagccgctc cgagcaggac gacggcctgt tgatccagaa accgattcgt
     7261 gtctccccag tagttcctgg tcgggggcga agccggatcg gcggcgcgca actgcaaacc
     7321 gttgatcggg ttgctgaaga gcagaacgag ggcgaggatg cgggccaggc aaggccgttt
     7381 caggtgggtg aatgtgctgt acatcttgcg ggtgaggatg aaccagatcg gacgccgacc
     7441 ccagcgcgaa tttccgactg cctgggaggc gaatcgcaag ctggcggaca acgcgaaaca
     7501 gactgaaaga cacactctcg ccattggctc ctcttttgca ttccgccgtt caatccgggc
     7561 cctggtgctg gctgaacgcc tcctgttggc cggccgtgaa ctgagcgttg aacgttgaac
     7621 gt
//
LOCUS       DAOQRO010000151         7608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9284_length_7608_cov_5.393486, whole genome shotgun sequence.
ACCESSION   DAOQRO010000151 DAOQRO010000000
VERSION     DAOQRO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9284_length_7608_cov_5.393486"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..3431)
                     /locus_tag="PKK20_07090"
     CDS             complement(294..3431)
                     /locus_tag="PKK20_07090"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNU99688.1"
                     /translation="MNCSSILVALALFSPAFDATLTAASESSAVKPLFADPPREYATA
                     PLWVWNDRLTEAQIRGTLRDLAGQRVKQVFVHPRPGLMTPYLSEEWFGLWRIALDEAA
                     RLDMNVWIYDENSYPSGFAGGWVPELMPSARGLGLKLGETKTAPPWHERILKVLQVED
                     DAYTRTEDVTAALKKERSLPEGRYFVASVEEAGNSPWHGNRCYVNLLTPGVTETFIEI
                     TLEAYRKNVGEHFGKRVPGVFTDEPNICPAGGLPWTRDLPEQFEQRWGYRLTDQLPGL
                     VLEVGDWRRVRHNYFQTLLDLFIERWAKPMYDWCETNGLEFTGHYWDHEWPRCIGVPD
                     NMAMAAWQHRPGVDTLMNQYAEHTHAQFGNVRYCREVSSIANQLGRKRTLVEAYGAGG
                     WDLRFVDMKRIGDWLEVLGLNTLDQHLSDITLRGARKRDHPQSFSYHEPWWEAYHLSA
                     AYFARLSAALSQGAQYNRLLVLEPTTTAWMYQGDEARLKEIGDSFSNLLMALEAAQIE
                     YDLGCEYVMARHGQPAGETVAVAAPTHRILHKELRVGQRTYHTVLLPPALENLNAATA
                     RLLDQVTCVRAYGRAESPRIDGQERTSTAPARGIDRAALDRYPARIVAELAHCLPFDG
                     FALHRDDGDRGILFHHRRQLDDGELVFIVNTSLEHPARGTLISDRQGIERWNLDTGAI
                     EPYAFVKGSGVTARFDLPPAGSLLLFLSRASRSPETRVWDDPLPVPAAGPLEIARLEP
                     NVLTLDYVDVTAGGESRTNVYFYQANRFAWQKHGMERNPWDSAVQFKDELIARTFPPD
                     SGFRASYRFVIEESVPSDLTIVIERPDLYRITCNGHPVKARKGAWWLDRAFGRIQLDA
                     VARVGENAVTIEASPFTMNHEIEPAYLRGSFALEPASSGFSVVPERPLHLKDGAGWND
                     QGHPFYSAGVAYRQDFRVDSKAPRSFIRLGRWNGSVARVRVNGDLAGYISAPPWELDV
                     TADLIAGVNRVEVTVLGTLKNTLGPHHNNPPLGSAWPAMFHAAPEQGPPRGGLYHTVG
                     YGLFEPFALLQSSPR"
     gene            complement(3540..4688)
                     /locus_tag="PKK20_07095"
     CDS             complement(3540..4688)
                     /locus_tag="PKK20_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99689.1"
                     /translation="MKTLNVGLIGCGFMGRAHSNAHRKVWNFFDLEYRPVLKAICDSN
                     EERAKAHAARWGFESVETDYRKLLARDDIDMIDICLPNNMHAEVAIAAAKAGKMILCE
                     KPLARNGKEAEGMVKAVEKAGVPNFVSFNYRRIPAVSLAKKLIDEGRLGKIFHYRAKF
                     LQDWTISKDVPQGGDSLWRLDVKAAGSGVTGDLLAHCIDTALWLNGRIESVTAMTETF
                     VKERKHQLTGKMQKVGIDDACAFLCRFANGSLATFESTRYARGHKALYTFEINGEKAS
                     IAWDLHDLHRLAYFDHRDEGKIRGWRSIHVTDHGGDHPYMDKWWVPGLNIGFEHSFVH
                     QLADFLTGLAKGKPASPTFAEALETQYVCDAVLQSAKTAKWEKVKKTT"
     gene            complement(4835..5464)
                     /locus_tag="PKK20_07100"
     CDS             complement(4835..5464)
                     /locus_tag="PKK20_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNU99690.1"
                     /translation="MIEFVLLAFSSLFVIVDPIAAVPAFLAMTPTDTPTQRIKIAGLA
                     CAVTAGMLIVFAWVGNYIFRFLGITMPAFQMAGSIVLLLVALDMLKAQRSRVHETSEE
                     TDAGTEKQDIAIAPLAVPMLAGPGAITTAILLHNQAGSSIAKQIALFFCILAVAGVSY
                     VTLRLSAHGAKWLSPIVLRLITRIMGLLLVAIAFQFVFNALEAMKGKLL"
     gene            complement(5457..7061)
                     /locus_tag="PKK20_07105"
     CDS             complement(5457..7061)
                     /locus_tag="PKK20_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99691.1"
                     /translation="MNLETRVDMGEIRSKNHVEAQAVRADFPDPGPQRNEVTTGGEAM
                     SALSAERTARAVRAALPPGGLFAGLRWRIAPASFPLDRALAADLERLGRVLLQFNRAV
                     NLLYRQSVAGKQPAWVAEWLDRGKPSELIAWQRHPSFKNDLPRVIRPDILLTDEGFVI
                     TELDSVPGGIGLTAWLNRTYAALESAGAGGCGRPDLDAAGGAPAEGRGRGGRISSELA
                     RSVGILGGPRGMVDGFTSIFGDAPRVHIVVSQEAATYRPEMEWLTGELESGEWGVKSG
                     ECGGQRAEYRVRDAEFDRVQSGDAVYRFFELFDLENVRAARRLFELASARRIRLTPPP
                     KPIFEEKMLFALLWNRNLRGFWRQELGESFVRRLLACVPVTWVMDPAPLPPHAALPGL
                     DLTDWRQLKGLSQRERDLVLKVSGYSDQAWGARSVALGSDLSAGDWGAAVDHALRSFA
                     RSPYVLQRYHKPRTVAATWFDFERGQTIPMAGRARLCPYYFVAGEGEAARARLGGVLA
                     TICPADKKIIHGMQEAILAPCRVDDD"
     gene            complement(7183..>7608)
                     /locus_tag="PKK20_07110"
     CDS             complement(7183..>7608)
                     /locus_tag="PKK20_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99692.1"
                     /translation="TLANVVRLRAEQFLGDAGERRSVIASQLDILPTILGILGIDEPH
                     QAFGRNLLRLPPDDPGHAYLKQSGDPVVGYLEGDWLSVRALGQASQLYRIDLGDPPSA
                     SEDLTVRELARAAALDKRLTAFTVTGLYTLTKQLMAPHP"
BASE COUNT         1365 a         2447 c         2445 g         1351 t
ORIGIN      
        1 ccccgccccg gagggctggg gggctttggc tcgctggctt cgttgctcct cagccacaga
       61 ggtcaaggct atgctacttc gccgcgcctc gtcagcgagc caagtcctct ccagcaaaat
      121 cggaagtagt ttttgcacaa accttaagga cattgcttcg ctcctcgcac aaaagcaagg
      181 ccgcaggagg agtccgccgc atgagccagc gccaattcat gaagtcgaaa aggtcggagt
      241 ggggacggct cgtcccccgc catgtcaagt gaagaccgcg cggaacgcgc gggttaccgt
      301 ggacttgact gcagcagggc gaacggctcg aagagaccgt acccgactgt gtgatagagg
      361 ccgcctcgag gcgggccctg ctcaggggcc gcgtggaaca ttgccggcca ggcgctgccc
      421 aacggcgggt tgttgtgatg cggtccgagc gtgttcttaa gagtgccgag aaccgtcacc
      481 tcgactcgat tcacgcccgc gatcaagtcc gcggtcacat ccagttccca cggaggggcg
      541 ctgatgtagc cggccagatc cccgttcacc cgcacgcgcg caacgctccc gttccatcgc
      601 ccgagccgga tgaacgatcg cggggccttc gagtcgacgc ggaaatcctg acgatacgcg
      661 accccggcgc tgtagaaggg atggccttgg tcgttccagc ccgccccgtc cttcaaatgc
      721 agaggtcgct cgggaaccac gctgaagccg gaagaggcgg gctcgagcgc gaacgagccg
      781 cgcagatacg ccggctcgat ctcatgattc atggtgaacg gcgatgcctc gatcgtcacc
      841 gcgttctcgc cgacccgggc gacggcatcg agttggatcc ggccaaaagc ccggtcgagc
      901 caccacgccc ccttcctggc cttcacggga tgcccgttgc acgtgatccg ataaagatcg
      961 ggccgctcga tcacgatggt caggtctgaa ggcaccgatt cctcgatcac aaaccgatag
     1021 ctcgccctga aaccgctgtc gggcgggaat gtcctggcaa tcagttcgtc cttgaactgc
     1081 acggcgctgt cccacgggtt acgttccatt ccgtgcttct gccaggcgaa ccggttggcc
     1141 tgatagaagt agacattggt ccgcgactcg cctccggcgg tcacgtccac gtaatccagg
     1201 gtcagcacgt tgggttcgag ccgagcgatt tcgagcgggc cggcggcagg cacgggcagg
     1261 ggatcgtccc agaccctggt ctccggagag cggctggcgc gggagaggaa caggagcagg
     1321 ctgcctgcag gcggaaggtc gaaacgggcc gtgacgccgc tgcccttgac gaaggcatac
     1381 ggctcgatcg ccccggtgtc gaggttccat cgttcgatgc cttgtcggtc gctgatcaga
     1441 gtgccgcgcg cggggtgttc gagacttgtg ttgacgatga acaccaactc gccgtcgtcg
     1501 agctgccggc gatgatggaa caggatgccc cgatcgccgt cgtcacggtg gagagcgaag
     1561 ccatcgaagg ggaggcagtg tgcgagttcg gccacgatcc gggccgggta gcgatcgagg
     1621 gcggcgcggt cgatgccgcg ggctggggcg gtcgaggtcc gttcctggcc gtcgatgcgt
     1681 ggcgattcgg cgcggccgta ggcgcggacg cacgtcacct gatcgaggag cctggccgtg
     1741 gccgcgttga ggttttcgag ggcgggcggg aggaggacgg tgtggtaggt gcgttggccg
     1801 acgcgcagtt ccttgtggag gatgcggtgg gtgggcgcgg cgacggcgac cgtctcgccc
     1861 gcgggttgcc catgccgggc catgacgtac tcgcagccga gatcgtattc gatctgagcg
     1921 gcttcgagcg ccatcagcag gttcgagaac gaatcgccga tctccttgag cctcgcttcg
     1981 tccccctggt acatccaggc ggtggtcgtc ggctcgagca ccaacaggcg attgtactgc
     2041 gcgccctggg acagcgcggc gctgaggcgg gcgaaatagg cggcggagag gtgataggct
     2101 tcccaccacg gctcgtggta ggagaacgat tgggggtgat cgcgcttgcg ggcgccgcgc
     2161 agggtgatgt cgctgaggtg ttggtcgagc gtgttcaggc ccagcacctc gagccagtcg
     2221 ccgatgcgtt tcatgtcgac gaaccggagg tcccacccgc ccgcgccata ggcctcgacc
     2281 agcgtgcgct tgcggcccag ttggttggcg atgctcgaca cttcgcggca gtaccgcacg
     2341 ttgccgaact gggcgtgcgt gtgctcggca tactggttca tgagcgtgtc aacgcccggt
     2401 cgatgctgcc acgccgccat cgccatgttg tcgggcacgc cgatgcaccg cggccattcg
     2461 tgatcccagt aatgccccgt gaattcgagt ccgttcgtct cgcaccagtc gtacatcggc
     2521 ttcgcccaac gctcgatgaa cagatcgagc agggtctgga agtaattgtg tcgaacgcgc
     2581 ctccaatcgc ccacttcgag gaccaggccc gggagttgat cggtcaggcg gtagccccaa
     2641 cgctgctcga actgctccgg cagatcgcgc gtccacggca atccgcccgc cgggcagatg
     2701 ttgggttcgt cggtgaacac cccgggcacg cgcttgccga agtgctcgcc gacattcttg
     2761 cgataggcct cgagggtgat ctcgatgaac gtctcggtca ccccaggcgt gagcaggttg
     2821 acataacacc ggtttccgtg ccatggcgag ttgcccgctt cctcaaccga tgcgacaaaa
     2881 taccggcctt cgggcaggga cctttccttc ttgagcgcgg cggtgacgtc ttcggtcctg
     2941 gtgtaggcgt cgtcttcgac ttgcagcacc ttgagaatcc gctcgtgcca gggcggggcc
     3001 gttttggtct cgcccagctt caaacccagt ccccgcgccg aaggcatcag ctcgggcacc
     3061 cacccgccgg cgaagcccga cggatacgag ttctcgtcgt agatccagac gttcatgtcg
     3121 agacgtgccg cctcgtcgag ggcgatcctc cataagccga accattcctc cgacagatag
     3181 ggcgtcatca gacccggacg cgggtgaacg aagacctgtt tgaccctttg cccggccagg
     3241 tcgcgcagcg tcccccggat ctgggcttcg gtgagcctgt cgttccagac ccagaggggc
     3301 gccgtggcat actcgcgcgg cggatcggca aaaaggggct tgacggccga ggactcggac
     3361 gcggccgtca gcgtcgcgtc aaacgcggga gagaagagcg caagcgcgac aaggatcgag
     3421 ctgcaattca taggaatatg ccaatgcgcc cctgcggaac ggcagtcccg ccgaccttcg
     3481 agtcggcggg cgtctgcagc tcggtgaact ggcgggtgcc accgaattcc cagcgcgcct
     3541 tacgtggtct tcttgacctt ctcccacttc gcggttttgg cggattgcag gacggcgtcg
     3601 cagacgtact gggtttccag cgcttcggcg aaggtgggac tggctggctt gcctttggcc
     3661 aggccggtca ggaaatccgc aagctgatgc acaaacgaat gctcgaaccc gatgttcagc
     3721 cccggcaccc accacttgtc catgtacgga tgatcgccgc cgtggtcggt cacgtggatc
     3781 gatcgccagc cgcggatttt gccttcatcg cggtgatcga aataggccag gcggtggaga
     3841 tcgtgcagat cccaggcgat gctggccttc tcgccattga tctcgaaggt gtaaagcgcc
     3901 ttgtggccgc gggcgtagcg cgtcgactcg aatgtcgcca gcgaaccgtt ggcaaatcgg
     3961 cagaggaagg cgcaggcatc gtcgatcccc accttctgca tcttgcccgt gagttggtgc
     4021 ttgcgctcct tcacgaaggt ctcggtcatc gccgtcaccg attcgatccg cccgttgagc
     4081 caaagggcgg tgtcgatgca gtgggccagc aggtcgccgg tgacgccgct gcccgccgcc
     4141 ttcacatcca gccgccacag cgaatcgcca ccctgcggaa cgtccttcga gatggtccag
     4201 tcctgcaaaa acttcgcgcg ataatggaat atcttcccaa ggcgcccctc gtcgatcagc
     4261 ttcttggcca gcgaaacggc cgggatgcgg cggtaattga aggagacgaa gttgggcacg
     4321 ccggccttct cgacggcctt gaccatgcct tcggcctcct tgccgttgcg ggcgagcggc
     4381 ttctcgcaca ggatcatctt gccggccttg gccgcggcga tcgccacctc cgcgtgcatg
     4441 ttgttcggca ggcagatatc gatcatgtca atgtcgtcgc gcgccaggag cttgcggtaa
     4501 tccgtctcga cggactcaaa cccccaccgg gcggcgtggg ccttggccct ttcctcgttc
     4561 gagtcgcaga tcgccttcag gacaggcctg tattcgaggt cgaagaaatt ccagactttg
     4621 cggtgtgcgt tcgagtgtgc ccggcccatg aagccgcagc cgatcaagcc gacattgagt
     4681 gttttcatac gtgatatgct tccccctcgg tttgcaggaa cagccgcccg actgcaagga
     4741 tgaaatgcgc ctggcccgca cagcagtccg cttgtgaagc ctgtccgccc tctgcctgga
     4801 cggggtgtgc cacggacggg gtgtgccacg cgggctacaa aagcttgccc ttcatggctt
     4861 cgagggcgtt gaacacgaac tggaaggcga tggccaccag caacaggccc atgatccgcg
     4921 taatcaggcg cagaacgatc gggctgagcc atttggcgcc atgggccgag aggcggagcg
     4981 tgacatagct cacgccagcc acggccagga tgcagaaaaa gagcgcgatc tgcttggcaa
     5041 tgctgctccc ggcctggttg tgcagcagga tcgcagtcgt gatcgccccg gggccggcca
     5101 acatcggcac ggccagcggc gcaatcgcga tatcctgctt ctcggtgccg gcgtcggtct
     5161 cttccgacgt ttcatggacg cgcgaccgct gggccttgag catgtcgagg gcgacgagca
     5221 acagcacgat cgagcccgcc atctggaagg ccggcatcgt gatgcccagg aaccggaaga
     5281 tgtagttccc gacccaggcg aacacgatca gcatgcccgc cgtcaccgca cacgccaggc
     5341 cagctatctt gattcgctgt gtgggcgtgt cggtgggcgt catcgccaga aacgccggca
     5401 ccgccgcgat cggatcgacg atcacgaaca gcgaactgaa ggccaatagg acaaactcaa
     5461 tcatcgtcca cacgacacgg ggcgaggatc gcctcctgca tcccgtgtat gattttctta
     5521 tccgccgggc agatggtggc cagcaccccg cccaaccgcg cccgcgcggc ctcaccctcg
     5581 cccgcaacga aataataggg acatagccgc gcgcgtccag ccataggaat cgtctggccg
     5641 cgctcgaaat cgaaccaggt cgcagccacg gttctcggtt tgtgatagcg ctgcagcaca
     5701 tagggagaac gcgcgaagct ccgcagcgca tggtcgaccg ccgcgcccca atcgccggcc
     5761 gacaaatcgc tgcccagagc cacactgcgg gctccccagg cctggtcgga gtagccggac
     5821 accttgagga cgagatcgcg ttcgcgctgc gacaggccct tgagctgccg ccagtcggtc
     5881 aaatcgagcc ccggcaatgc ggcatgcggc ggcaacggcg cggggtccat gacccatgtc
     5941 acgggcacgc aggccagcag gcgccgcacg aaactctccc ccaactcctg ccgccagaat
     6001 ccccgcaggt tcctgttcca caacaatgcg aacagcatct tttcctcgaa gatcggcttg
     6061 ggcggcggtg tgagccggat gcgcctggcc gacgccaact cgaacaggcg gcgggcggcc
     6121 cggacattct cgaggtcgaa caactcgaag aaccgataca ccgcatcccc cgactggacc
     6181 ctatcgaact ccgcatcccg cactcgatac tcggcccttt gccctccgca ctccccgctc
     6241 ttcactcccc actctccact ctcgagttcc cccgtcagcc actccatctc cggacgatag
     6301 gtagccgctt cctgagagac gacgatatgg acgcgtgggg cgtcgccgaa gatcgaggtg
     6361 aatccatcga ccatgccgcg cgggccgccg agaatgccga cgcttcgagc gagttcgctc
     6421 gagatgcgcc ccccacggcc gcggccctcg gccggggcgc ctccggccgc atcgagatcc
     6481 ggccggccgc agcctccggc ccccgcgctc tcaagcgctg cataggtccg gttcagccac
     6541 gccgtaagcc cgatgccgcc cgggacggag tccaattccg tgatgacgaa tccttcgtct
     6601 gtgagcagga tatccggccg gatcacgcga ggcagatcgt tcttgaacga cggatgccgt
     6661 tgccaggcga tgagttcgga cggcttgccg cgatcgagcc attcggccac ccacgcgggc
     6721 tgcttgccgg ccacgctctg tcgatagagc aggttcaccg cccgattgaa ctggagcaag
     6781 acgcgaccca gacgctcgag atcggccgcc agcgcgcgat cgagaggaaa cgacgcaggc
     6841 gcaatgcgcc atcgcagccc ggcgaacaat ccgcccggcg gcagggcggc gcggacggcg
     6901 cgggctgtgc gctcggcgga cagggccgac atggcttcgc cccctgtcgt gacctcattg
     6961 cgttgaggtc cgggatccgg gaaatcggct cgaaccgctt gggcttcgac atggttcttg
     7021 ctacggattt ctcccatatc gactcgagtc tcgaggttca tacccgccgc tttcggggac
     7081 ggcaaaggaa aaaagccagc ggtctgccac taccagtttt cagtttcgca gttgaatgaa
     7141 aagcccctgc gtgtctgcgc cgacctctca tccgcgtggc tttcaaggat ggggcgccat
     7201 caactgcttg gtcagcgtgt agagcccggt cactgtgaag gctgtcaggc gtttgtcgag
     7261 tgcggccgcg cgggcgagtt cgcggacggt caggtcctcg ctggcagacg gcgggtcacc
     7321 gaggtcgatg cgatagagtt gggaggcttg tcccagagcg cgcaccgaca accagtctcc
     7381 ctcgagatag ccgaccacgg gatctccgga ctgtttcaag taggcatgac ccggatcgtc
     7441 gggaggcaga cgcaaaaggt tgcgtccgaa agcctgatgc ggctcgtcga ttcccaagat
     7501 gcccaggatg gtcggcagaa tgtcgagttg gctggcaatc acgctgcgac gttccccggc
     7561 gtcgccgagg aactgctcgg cacgaaggcg tactacgttc gcgagggt
//
LOCUS       DAOQRO010000152         7571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9358_length_7571_cov_4.280734, whole genome shotgun sequence.
ACCESSION   DAOQRO010000152 DAOQRO010000000
VERSION     DAOQRO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_7571_cov_4.280734"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PKK20_07115"
     CDS             complement(<1..1185)
                     /locus_tag="PKK20_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNU99693.1"
                     /translation="MITATTASGSNLEARLNTRYIWTICLVAALGGLLFGYDWVVIGG
                     AAKFYEAFFQLKDTAQAVAAESGFWAKLRTNAISPVGWAQSCALLGCLVGAIVSGPLS
                     DRFGRKPILILAAFNFVASSLGIAFAQSFPVFVTWRIMGGVSIGLASNISPLYISEIA
                     PATQRGLLVAINQLTIVIGILAAQFVNWCIARPIPDGMAADAFAASWNVTRGWRWMFG
                     ACAVPSLLFFVSALFVPESPRWLCKAGTGRRAERVLSRIGGASYASAAMAEIQATLTS
                     EDRRVRLGELLDRRVLVVLGLGIFLAVFQQWCGINVIFNYASEIFAQAGFNLNDALTN
                     IVATGVVNLVFVFVALFTVDRLGRRPLMLFGAAGLALIYLAIGACYHIKGAGTPVPPI
                     LFL"
     gene            1520..2671
                     /gene="gcvT"
                     /locus_tag="PKK20_07120"
     CDS             1520..2671
                     /gene="gcvT"
                     /locus_tag="PKK20_07120"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNU99694.1"
                     /translation="MLKRTPLHDLHRQLEARMVDFGGWEMPVQYTGIVQEHLAVRQSA
                     GLFDISHMGEMLVSGPGATEFLNHALTNDIRKLAIGQGQYTLLCQEHGGVVDDLYVYR
                     IDTDEFLMIVNASRIEADWDWLQIRVDHAPTRDGFRLRNASSEYGAVAVQGPAVAQFI
                     DTAIEGGSMGCTLVPGASALKKNEIGAFVFQGASVYVARTGYTGEDGFEVVAPTAAIA
                     PIWRALIEAGAPFGLKPAGLGARDTLRTEMGYPLYGHELTEATTPIEAGLAFFVALDK
                     GDFVGRDVLLRQKAEGVSRRCVTFRMADKSPPPRPGYIVWSADPAAAPIGKVVSGTQS
                     PSLGVGIGMAYVATAMAQPGTALAIEIRGRRFPAAIEKKPLYRRPAARP"
     gene            complement(2831..3679)
                     /locus_tag="PKK20_07125"
     CDS             complement(2831..3679)
                     /locus_tag="PKK20_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNU99695.1"
                     /translation="MKGIPVLHVEADGIARAWEESLVALHERGCNVRSEYDKPDDPPS
                     KDATMIITIADPLSEPMIHRDFPGGPVELQEYVMEVCEGIKDHLVRDPDDPHDTRWEY
                     TYHQRLFAYTAPTLEPADQIEVLCQKLARTPHSRRAQAITWKVWEDNDCYDPACLQSI
                     WCRLTEEQRRQVLSMNVRFRSNDAYKAAFMNIFALVQLQKRIADRIGSLTGRPVEPGR
                     YVHVADSYHIYGSYFREFEGRFLGALRKRSFEQRTLRYADLREMMEEARPMILEKAAA
                     MGKGTA"
     gene            complement(4006..5139)
                     /locus_tag="PKK20_07130"
     CDS             complement(4006..5139)
                     /locus_tag="PKK20_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU99696.1"
                     /translation="MNTLLIIDDDVGTRETLQALLASEERRLEFVSNPTKALAIATQL
                     QPDLILLDVLMPGLDGFEICRQIRANPALAEVPILLLTVLDDRDSRLAGIAAGADDFV
                     SKPFDRAELTMRVQSILRLNRYRRLLEERTKVDQLTEYALVVSHELKAPLRGMRQLAD
                     WIARDHAARLDSEGQQLFTLLKERVRELDRTIDGLLECARVGKTHQVERQIATGELVR
                     EIAEQLALPPLVKITIDPDLPTVNASREQLQQVFQNLLDNALASLDQPQGRLRVSATR
                     RVAAWEFLVADNGRGIPARFLDRVFEPYWRMRRSGDKPGVGLGLTLVKRIVEDRGGAA
                     WVQSREGRGTIVHFTWPDERADRQQPPSQAGRGPKRPHRSAQP"
     gene            complement(5136..>7571)
                     /locus_tag="PKK20_07135"
     CDS             complement(5136..>7571)
                     /locus_tag="PKK20_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99697.1"
                     /translation="LVGAVGLVGVQGLTVQRIVEELAVISVVSLGFLGFVLRRQSRLK
                     AEYRLIEGVYREAIGAAGAVPYRKDIATNLYAFMGDGIEGITGYPAHEMTPTLWHSLI
                     LEEAERGGSGPEHPPPVARQKGVGGPLLWRCDLRIRTRSGEERWVADSSTQVLGRDGR
                     PTAWVGMLQDITETKRIEERSTQRRQVLELIATGAPLAAALEAVATLWENHIAGSTCC
                     IAVANAAGRLEFLNMRRAAAEHRACPTCFGPAILDRWSAHQNRAVERVPDAFTASEPW
                     QVGLDPTGADGVDVLSWSMPIDGVEKQLLGVVCCCADRAKLLDAKESDTARLVVSLAR
                     LAIERHRNNEALRGRTKRLAEVNKRLVELARSPHIGGGAWEESAREITEAGARAIQVA
                     RVGIWLLTSGGEELCCEDRFDLDTGRHESGERLSVARYPAYFNACGLSRIIAVRQTAI
                     DPRTEELRKDYLARHGIVSLLDAPIRQAGRLLGVFSFEHTGLAREWTPEEEVFAASCA
                     DFVVLAIEARERCRAEALVRQLNVDLEQRVEERTRQLSQANAELARAAKLKDESLTNM
                     SHELRTPLVSILGLAEALQTDSPGALNTCQRHYLRTIEESGRHLLQLINDALDVAKSQ
                     AGRIRLQLDLCDVGGVCESALRLVKESALAKGQILSVTVRPPGISCVADARRLKQILV
                     NLLSNAVKFTPRGGRLGLAVEGDEANRALRFTVWDTGVGIAAEDLPRVFQPFVQAGER
                     LSHTPAGTGLGLVLVRRMAELHGGHVHATSTPGTGSRFSVVLPWRTRLETECGLRDGL
                     DTEGPPPGTAVHA"
BASE COUNT         1338 a         2493 c         2424 g         1316 t
ORIGIN      
        1 gaggaagagg atggggggga ccggcgtgcc cgctcctttg atgtgataac aggccccgat
       61 cgccaggtag atgagcgcca agcccgccgc cccgaacagc atcagcggcc tgcgccccaa
      121 ccggtccacg gtgaacagag ccacgaagac gaagaccagg ttgaccacgc ctgtggcgac
      181 gatgttggtg agggcgtcgt tgaggttgaa gccggcctgg gcgaagatct cgctggcgta
      241 attgaagatg acattgatgc cgcaccattg ctggaacacc gccaggaaga tccccaggcc
      301 cagcacgacc agcacccgcc gatcgagcag ttcgcccaaa cgaacgcgcc tgtcttcgct
      361 cgtcaaagtc gcttgaatct cagccatcgc cgccgacgcg taggacgcgc cgccaatgcg
      421 cgagaggacc ctctccgccc gacggcccgt tccggctttg cagagccagc gcgggctttc
      481 cggcacgaac agggcgctga caaagaacag cagcgacggc actgcgcagg ctccaaacat
      541 ccagcgccag ccgcgcgtca cgttccacga cgccgcgaat gcgtccgcgg ccatcccatc
      601 cgggatcggg cgggcgatgc accagttcac aaattgcgcg gccaggatgc cgatgacgat
      661 cgtgagctgg ttgatggcga caagcagccc gcgctgtgtg gcgggggcga tctcggagat
      721 gtacaacggg gagatgttcg acgccagccc gatgctcacg ccacccatga tccgccacgt
      781 gacgaacacc gggaagctct gggcaaaggc gattccaagc gacgacgcca cgaagttgaa
      841 cgccgccagg ataaggatcg gtttgcgtcc gaatcggtcg ctcaaaggtc cggacacaat
      901 cgcgccgacc aggcacccga ggagcgcgca actttgcgcc cagcccaccg gagagatcgc
      961 gttggttctg agcttggccc agaaaccgga ctcggcggcg acggcttggg ccgtgtcctt
     1021 gagttggaag aaggcctcgt agaacttcgc tgccccgccg atcaccaccc aatcgtaacc
     1081 gaacagcagg ccgcccagcg cagccacgag acagatggtc cagatgtaac gtgtgttgag
     1141 acgcgcttcg agattcgaac cggaggcggt cgtggcggtg atcatcggcc ccgcagtgtg
     1201 ccgggggcgc cggagaaagc aaggcgcgag gcctcgcccg gttgtctgct cagtgcattt
     1261 gcaagttgtc ggcgggaatc gagtttgcgc cgcgtggggc ttgcctgccg cgcctgcctg
     1321 tgccgtgccc gacacggcag acaggttgct gcgctttgcg gcgcaggcag ggcacagggc
     1381 ttgcaccggg agtctccatc cgttgtaagc ccggggccct caccgggcga cagaatgccg
     1441 acaactccca tatgcaccgt cgcccgcacc gtcgcctgct tgacgggcgc gggaaggttg
     1501 gtagcgtctg aggcacgcta tgctcaaacg gaccccgctc cacgatttgc accgccaact
     1561 cgaggcccgc atggtcgatt tcggcgggtg ggagatgccc gtccagtaca ccggcatcgt
     1621 ccaggaacac cttgcggtgc gccaaagcgc cgggttgttc gacatctccc acatgggcga
     1681 aatgctggtg agcggtcccg gagcaaccga gtttctgaac cacgcactga ccaacgacat
     1741 ccggaagctg gcgatcgggc aggggcagta cacgctgctc tgccaggagc atggcggcgt
     1801 ggtggatgac ctgtacgtct accggatcga caccgacgaa ttcctgatga tcgtcaatgc
     1861 ctcgcgcatc gaggcggatt gggactggct ccagattcgc gtcgaccatg cccccacgcg
     1921 cgacggattt cgtctccgca acgcctcgtc cgagtatggc gccgttgcag tccaggggcc
     1981 ggctgtggcg cagttcatcg acaccgcgat cgagggcgga tcgatgggct gcaccctggt
     2041 gccgggcgcg tctgccctca agaagaacga aattggggcg tttgtgtttc agggcgcctc
     2101 cgtgtatgtg gcgcggaccg gttacaccgg tgaagacggc tttgaggttg tcgcgccgac
     2161 cgcggcgatc gccccgatct ggcgggcgct gatcgaggcg ggcgcgcctt tcggactgaa
     2221 gccggcgggt ctgggcgcgc gcgacactct gcgcaccgaa atgggctatc cgctctatgg
     2281 ccatgaactg accgaggcga cgacccccat cgaagccggc ctcgctttct ttgtggcgct
     2341 cgacaagggc gactttgtcg gacgggacgt gctgctgagg caaaaggccg agggagtatc
     2401 caggcgatgc gtgacgttcc gaatggccga caaatcgccg cctccccgcc ccggctatat
     2461 cgtttggagc gccgatccgg ctgcggcgcc gattggcaag gtggtcagcg gcacccaaag
     2521 cccgtccttg ggggtgggga tcgggatggc ctacgtggcg actgcgatgg cgcagccggg
     2581 cacggcgctg gcaatcgaga ttcgcggacg gcgcttcccg gcggcgatcg agaagaaacc
     2641 gctctaccgg aggcccgcgg cccgcccgta gccggttatt ccacacgctt cgcgaggaac
     2701 cggacccggc gcagttttgc ttttgcagcc cgcatagcag tcctcatgcg aggtttgtcc
     2761 gccctgtgca ctgacggggc gagaggaata tgcgggctcg gcaaagacgg cgggagtgcg
     2821 gcgcggtgac tcaggcagtc cctttgccca tggccgccgc cttttcgagg atcatcggcc
     2881 gtgcctcctc catcatctct ctcagatcgg cataacgaag cgtgcgctgc tcgaaggaac
     2941 gtttacggag tgcgccgagg aatcggcctt cgaactcgcg gaaataagag ccgtagatgt
     3001 ggtaactgtc cgcgacgtgg acgtagcgcc ctggctcgac ggggcgcccg gtcaggctgc
     3061 cgatgcgatc ggctatgcgc ttctgcagtt gaaccagggc gaagatattc atgaacgcgg
     3121 ctttgtaggc gtcgttggac cggaaccgga cgttcatgct caggacctgg cgccgctgct
     3181 cctcggttag tcggcaccag atgctttgaa ggcaggccgg gtcgtagcaa tcgttgtcct
     3241 cccagacctt ccacgtgatg gcctgggcgc gcctcgagtg gggtgttcgc gccagtttct
     3301 ggcaaaggac ctcgatctgg tctgcgggtt cgagggtggg cgctgtgtag gcaaacaagc
     3361 gctgatgata cgtgtactcc cagcgcgtgt cgtgcgggtc atccggatcg cgcaccaggt
     3421 ggtccttgat gccctcgcac acttccatga cgtactcctg caactcgacg gggcccccgg
     3481 gaaaatccct gtggatcatc ggctcgctca gagggtcggc gatcgtgatg atcatcgtcg
     3541 cgtccttgct tggcggatcg tcgggcttgt catactcgct ccggacgttg catccgcgct
     3601 catgcagggc aacgagcgat tcttcccaag cccgagcgat cccatcggct tcgacgtgga
     3661 ggacagggat gcctttcata agcggatgga gagttgacga gaggatcgga tgggctgcgc
     3721 gttcatgaca ggggaagatc tgtacagtgt tcttccgccc gcgcaaacag gaatcgtcga
     3781 ggaatcgtca aggcggtgca tcggcccgct gtcggtatcc tctcgcccgg tgagggcacc
     3841 gggtctacaa cggatggaga ctcccgctgt aggccgcgtg ccctgcctgc gccgcaaagc
     3901 gcagcaacct gtctgccgtg tcgggcacgg cacaggcagg cgcggcaggc aggctccacc
     3961 cggcgcaaac tcctttccca ccgacagctt gcagatgccc tgtcgtcaag gttgcgcgga
     4021 ccgatgcggg cgcttcggcc cccttccggc ctgggagggc ggttgctgtc gatcagcgcg
     4081 ctcatcaggc caggtgaagt gcacgatcgt gccgcgcccc tctcgggact ggacccaggc
     4141 ggcgccgccc cggtcctcga cgatccgctt gaccagggtc aggcccagcc ccacgccggg
     4201 cttgtcgccg ctgcgacgca ttcgccagta tggctcgaag acccgatcca gaaagcgcgc
     4261 cgggatcccg cgtccgttgt cagccaccag aaactcccag gcggcgactc gtcgcgtggc
     4321 gctgacccga agccgcccct gcggctggtc caaggaagcc agggcattgt cgagcaggtt
     4381 ctggaacacc tgctgcaact gctcgcggct cgcgttcacg gtcggcaggt cgggatcgat
     4441 cgtgatcttg accagcggcg ggagtgcgag ttgctccgcg atctcgcgca ccagttcacc
     4501 cgtggcgatc tgccgctcaa cctgatgcgt cttccccacc cgggcgcatt ccaggagccc
     4561 gtcgatcgtc cgatccagct cgcgcacccg ctccttcaga agcgtgaaca gttgctgccc
     4621 ctccgaatcg aggcgcgcgg cgtggtcccg cgcgatccaa tcggcaagct ggcgcatgcc
     4681 gcggagcggg gccttgagtt catggctgac gaccagggcg tactcggtga gttgatccac
     4741 cttggtacgt tcctcgagca gccggcgata gcggttcaac cggagaatgc tctggacacg
     4801 catggtcaac tcggcgcggt caaacggctt tgacacaaaa tcgtcggccc cggccgcgat
     4861 gcccgcaagg cgggaatccc ggtcgtccag aaccgtgaga agcaggatcg gaacttcggc
     4921 aagcgcagga ttggcgcgta tctgccgaca aatctcgaat ccgtccagtc ccggcatgag
     4981 gacgtccagg aggatcaggt cgggctggag ttgggtcgcg atggccaggg ccttagtggg
     5041 gttcgacacg aattcgagcc gtctctcttc ggacgccagc agcgcttgga gcgtttcccg
     5101 cgttccgacg tcgtcatcga tgatcagcaa tgtgttcatg cgtggacggc ggtcccgggg
     5161 ggcggcccct ccgtatcgag gccgtctcgg agtccgcatt cggtttcgag tcttgttcgc
     5221 caaggcagca ccacgctgaa gcggcttccg gttcccggcg tgcttgtggc gtgcacgtgg
     5281 ccgccatgca gttcggccat gcggcgcaca agcaccaggc caagaccggt gccggccggg
     5341 gtgtgggaga gccgctcgcc cgcctggacg aaaggctgaa acacgcgcgg caagtcctcg
     5401 gcggcaatgc ccaccccggt gtcccacacg gtgaaacgta gagcccgatt ggcctcgtcc
     5461 ccttccacag ccaggccaag gcgcccgcct cgaggagtga atttgaccgc gttcgagagg
     5521 agattcacga gaatctgttt caatcgccgc gcgtccgcca cacaactgat accgggcggc
     5581 ctgacggtga cgctcaggat ctgccccttg gcgagtgcgg actccttgac aaggcgcagg
     5641 gccgattcgc acaccccgcc cacatcgcac aggtcgagtt gaagtcgaat tcgtccggcc
     5701 tggctcttgg cgacatcgag ggcgtcattg atcaactgca gcagatgccg accgctttcc
     5761 tcgatggtgc gcaagtaatg gcgctggcac gtgttgagcg ccccgggcga gtcggtctgc
     5821 aaggcctcgg ccaagccgag gatgctgacc agcggtgtgc gcagttcgtg gctcatgttc
     5881 gtcagggact cgtccttgag cttcgccgcc cgcgcaagtt cggcgttggc ttggctcaat
     5941 tgacgggtgc gctcctccac ccgctgctcg aggtcgacgt tcaactggcg caccagcgct
     6001 tccgcccggc accgctcgcg ggcctcgatg gccagcacca caaaatcggc gcacgacgcc
     6061 gcaaacacct cttcctcggg cgtccactcc cgcgccaggc ccgtatgctc gaaactgaat
     6121 acgccgagca ggcgccccgc ctgccggatg ggcgcatcga gcaacgacac gatgccatgc
     6181 cttgccaggt agtccttgcg caactcctcg gtgcgcggat cgatggcggt ttgccgcaca
     6241 gcgatgatgc gcgacaaacc acacgcgttg aagtaggccg gataacgcgc caccgacagc
     6301 cgctccccgc tctcatgccg ccctgtgtcg aggtcgaacc tgtcctcgca acacagttct
     6361 tcgccgccgg acgtcagcag ccagatcccg acgcgggcga cctggatcgc ccgggctccg
     6421 gcttcggtaa tctcgcgcgc ggattcctcc cacgccccgc cgccgatgtg gggactgcgc
     6481 gcgagttcaa cgagccgttt gttcacctcg gccagccgtt tcgtcctgcc ccgaagcgcc
     6541 tcgttgttgc ggtgtctctc gatcgccaat cgagccaagg agaccaccag acgcgccgtg
     6601 tccgactcct tggcatccaa caacttggct cggtcagcac agcaacaaac tacaccgaga
     6661 agttgctttt cgactccatc gatgggcatg gaccaggaca aaacgtcgac tccatctgcc
     6721 cctgttggat ccagcccgac ttgccacggt tccgacgccg tgaacgcatc tggaacccgt
     6781 tcgacagccc ggttctggtg tgcgctccac cggtccagaa tcgcaggtcc gaaacaagtc
     6841 gggcatgccc ggtgctccgc ggcggcccgg cgcatgttca ggaactcgag gcgccccgcc
     6901 gcgttcgcga ctgcgatgca acaggtcgag cccgcgatgt ggttctccca gagggtggct
     6961 accgcttcaa gcgccgccgc aagcggcgcg cccgtcgcga tcagttcgag cacctgccgg
     7021 cgctgcgtag agcgctcctc gatcctcttg gtctccgtga tgtcctggag catgccgacc
     7081 cacgccgtcg gtctcccatc gcgcccgagc acctgagtcg agctgtccgc cacccaacgc
     7141 tcttcgcccg agcgcgtccg aatgcgcaag tcacatcgcc acagcagcgg tccgccaaca
     7201 cccttctggc gcgcaactgg gggcggatgc tcgggaccgc tgccgccccg ctccgcctcc
     7261 tcgaggatca ggctgtgcca aagcgtgggc gtcatctcgt gggctgggta acctgtgatg
     7321 ccctcgatcc catcgcccat gaaggcatag agattggttg caatgtcctt gcggtacgga
     7381 acggctccgg ccgcgccgat ggcctcgcgg tagacaccct cgatcagccg atactcggcc
     7441 ttcagtctgg attgccgccg cagcacaaag cccaggaaac caagcgaaac gaccgagatg
     7501 accgcaagct cttcgacaat ccgttgcact gtcaaccctt gcacaccgac cagccccacg
     7561 gcccccacta g
//
LOCUS       DAOQRO010000153         7517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9472_length_7517_cov_6.335567, whole genome shotgun sequence.
ACCESSION   DAOQRO010000153 DAOQRO010000000
VERSION     DAOQRO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9472_length_7517_cov_6.335567"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PKK20_07140"
     CDS             complement(<1..1276)
                     /locus_tag="PKK20_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HNU99698.1"
                     /translation="MQADGKAIVAGRFEFFHGRPISGLVRLDTDGGVDTRFALWPGVE
                     GAVNSVAIQQDGKIVFGGDFTSVAGQARHGAARVGPEGTLDEAFAPRVDGQRSRTIEV
                     VRVLEDGRLFIGGQFRTVESAPRACVARLKPDGTLDETFDPGAGVEDAFGIVQDLAPL
                     PDGGVVVAGLFTKFDGQTRPGLVRLDAEGNVVPEFNPDLDWSQGLVSVALVRRQEDGY
                     LLVAGRFDQLEGQALFGLARLQQTGVADPSFEGDSWRDVVVDAVRDLAIQPDGRIVVT
                     GDFDRFAESLHPGIARLEASGAPDETFDPGPGLRTAGRSAGVGMAVAVQADGRILVAG
                     QFSQIGTEVRHNLGRLHADGSADPDYSDSNRIVERVGSVNAITVQEDGRILVGGDFER
                     VDGQPRQALARVLPHGALDESFDAHVSKDGVVN"
     gene            complement(1615..2724)
                     /gene="mtnA"
                     /locus_tag="PKK20_07145"
     CDS             complement(1615..2724)
                     /gene="mtnA"
                     /locus_tag="PKK20_07145"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNU99699.1"
                     /translation="MKVTVGNRTRHYRTVDFDARHNTVRLIEQRLLPHRFKVVATPDY
                     RATAAAIRDMAVRGAGAIGATAAFGLAQGARAFRGANLDRFEACLATVYETLKAARPT
                     AVDPVNAMDQVRRRMAQGGTVAERQDLALAAAQAFADTNVRECEAIGRHGLDLIPEGA
                     RVLTHCNAGWLAFVDVGSATAPLYAAQAAGRSFHVYCDETRPRCQGASLTAWELAQQG
                     ISHQVIADNAAGHLMQRGEIDLVIVGSDRVLARTGEVANKIGTYTKAVLARRHRLPFY
                     VAIPLSTIDWEMRSGRAIPIEERAEEEVLGAWGLAAGNKRTWVRVANPTSGARNPAFD
                     VTPPDLITGIITPLGVYRPRQLARLRRRLEGCETR"
     gene            complement(2734..3144)
                     /locus_tag="PKK20_07150"
     CDS             complement(2734..3144)
                     /locus_tag="PKK20_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99700.1"
                     /translation="MAETRREKLRRDERRNLDIEIGFLEGLTRRDPFYDDALRLLADD
                     YSRRGRFEDGLRVDAQLVRLRPEDPLAHYNLACSYALTNQFDAAYAALNLAIDRGYRD
                     FRWLARDPDLASFRKHPLYRKLRARVKALRIEMR"
     gene            3211..5682
                     /locus_tag="PKK20_07155"
     CDS             3211..5682
                     /locus_tag="PKK20_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNU99701.1"
                     /translation="MPKRVLTSLTMGRAWETLAFMIRMRFVGRLAFALGLAAWLPAVP
                     GAALRAASSPSAATDAPGGLRIWFTEPATNWEKQAFPIGNGRLGAMVFGGTGRERIQL
                     NEDTLWSGGPRDTTNPEALKHLPEVRRLLFDGRPDEAVKVAQEHLMGRPMRIKPYQSL
                     GDLRLDFPGHEAAEDYRRELDLERAVVRIQYRVGEIRHTRELFASHPDQVLVLRLTCS
                     KPGGLTFFATLDREQDFRTSTRSPDRVLLQGQVDGGQGTEYCAVLQAHAQGGQMFAPA
                     RRLEVRNATSVTLVLGAATSFGGRDALQVATDDVEAAIRKPYAALMAAHVRDYQALFN
                     RVSLDLGGADAAVQPTDARLAAMKKGGNDPHLLATYFQFGRYLLISCSRPGDLPANLQ
                     GLWAEGMNPPWNSDYHLNINLQMNYWPAETTALSECHQPLFELIESLRESGRRTARVH
                     YGARGWVAHHLTDVWGFTTPADGAGWGLWPMGAAWLCQHLWEHFAFTGDRTFLARRAY
                     PPMKEAAEFFLDFLVEDSKGRLVSGPSSSPENTYRLPNGTQGGLCMGPSMDSQIIRDL
                     FSNCIRASEILRIDTEFRGRLAATLAKLPPTQIGRHGQIMEWSEDYDEPEPGHRHISH
                     LFALHPGREITRTGTPDLAQAARKTLERRLAHGGGHTGWSRAWILNFWARLGDADKAH
                     ENLLALLRHSTAPNLLDLHPPFQIDGNFGGTAGLAEMLLQSHAGEIELLPAKPSAWAD
                     GHVSGLRARGGFAVDLAWRGGQFESGRIESLGGNVCRLRCPVPVTVRSGSARGAVVKT
                     RPLATQAIEFATKRGGQYFLSRDAH"
     gene            5672..6661
                     /locus_tag="PKK20_07160"
     CDS             5672..6661
                     /locus_tag="PKK20_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNU99702.1"
                     /translation="MHTDLQPLEAAIGRHQARLEALRAEMARSIVGQQTLIERLLVGL
                     LANGHVLLEGVPGLAKTATVNALGQCLHLKFARVQFTPDLLPGDLIGNLVFNPRDNSY
                     HTRKGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGEQSFPLPSPFLVLATMNP
                     IEQEGTYSLPEAQVDRFMLKVVVTYPTEPEERQIMALMARTAPRPPLATVVRPEDILA
                     MRALVDQVFVDEQVSDYIVRLVFATRNPSPVAPRLAPFIRFGASPRASINLSLASKAL
                     AFLRGRPHVTPADVKAVAADVLRHRVLTTFEADADRITSEHIVSELLAQVPVP"
     gene            6852..>7517
                     /locus_tag="PKK20_07165"
     CDS             6852..>7517
                     /locus_tag="PKK20_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNU99703.1"
                     /translation="MRRVRLVELRARRFVEDLLAGPYRSVFRGRGLEFDQVRAYEPGD
                     DVRSIDWHTTARTGAVQIKQGIEERSLTVMLAVDVSASGDFGSVPQSKRELAAELAAV
                     LALSAVLNNDRVGLLLFTDAVECYLAPRTGRRQALAIVSAILTHEARSPRTSLRQALQ
                     ILGNALQRRATVFVLSDFCDEGFERALRLAARRHDVVAMCVHDPVEARLPEVGWLVCE
                     DAET"
BASE COUNT         1256 a         2492 c         2503 g         1266 t
ORIGIN      
        1 cattgaccac gccgtccttg ctgacatggg cgtcgaacga ttcatccagc gccccatgcg
       61 gaaggactcg cgccaaggcc tggcggggct ggccatcgac gcgttcgaaa tccccgccga
      121 ccaaaatccg gccgtcctcc tgcaccgtga tcgcattcac cgagccaacc cgctcgacga
      181 ttcggttgga atccgaatag tccggatcgg ccgatccgtc cgcgtgcagc cgcccgaggt
      241 tgtggcggac ttccgtaccg atctgcgaga attgccccgc taccaggatg cgcccgtcgg
      301 cctggactgc gacggccatg ccgacgcccg ccgatctccc ggcggttcgc aggcctggcc
      361 ccggatcaaa tgtctcgtcc ggtgcgccgc tggcctcgag ccgtgcaatc cccggatgca
      421 gactctctgc gaaccgatcg aagtcccccg ttaccacaat ccggccatcc ggttgaatcg
      481 ccagatcgcg caccgcgtcc acaacgacat cgcgccagct atcgccctcg aaactggggt
      541 ctgcgactcc cgtctgctgc aagcgggcca ggccgaagag cgcctggccc tccaactggt
      601 caaagcgccc ggcgacaagc agataaccgt cctcctgacg gcgcaccaac gcgacgctga
      661 ccaatccttg gctccagtcc aggtcggggt tgaactcggg caccacgttg ccttcggcgt
      721 caagccggac caaaccgggg cgtgtttgcc cgtcgaactt cgtgaacaac ccggcaacca
      781 caacaccccc gtcgggcagc ggcgccaggt cctgcacaat cccgaaggcg tcttcgactc
      841 ccgcgccggg atcgaatgtc tcatcgaggg tgccgtcggg cttcaaccgc gccacgcagg
      901 cgcgcggggc cgattcgacc gtgcggaact ggccgccgat gaagaggcgc ccgtcttcga
      961 gaacccgcac cacctcgatt gtgcgcgacc gttgcccgtc cacacgcggc gcgaacgcct
     1021 catcgagggt cccctccggg ccgactcggg ccgcaccgtg ccgcgcctgg ccggccacgc
     1081 ttgtgaaatc gcctccgaat acaatcttgc catcctgttg gatcgcgacg gaattgacgg
     1141 cgccctcgac tccgggccag agcgcaaaac gcgtgtccac cccgccgtcc gtatcgagcc
     1201 ggaccaaccc gctgatgggg cgtccgtgga aaaactcgaa ccggcccgcg acgatcgctt
     1261 tgccatcggc ctgcactgcg acagctctca gttcggcgcc ggctgtggtg gtgaaagatg
     1321 gatgaaagtg aggatcgagg cggggcgatg ggctggccca ggccagcgcc gcaatcgaga
     1381 acgctgccgc caatgatgcg ccgagccggc ccggccacgc tcgggctggc agctcaactc
     1441 gattggcaca gcacacggat accacagatt tcagcagcag gatgggccca ttccgtccgc
     1501 aacagcactc tggcagaacc tcgttgagcc atccattaag gggttgataa agagcagatg
     1561 ttgtgccggg agcctccgcc gcactcgcac gggcgcatcc ccgccgccgt ccgatcagcg
     1621 ggtctcgcag ccttcgagcc tgcgacgcag ccgggcgagt tgccgaggcc ggtagacgcc
     1681 cagcggcgtg atgattccgg tgatgagatc gggcggggtg acgtcgaacg cgggattgcg
     1741 ggcgccactg gtcgggttgg cgacgcggac ccaagtccgt ttgtttcccg cggcaaggcc
     1801 ccaggccccg aggacttcct cttcagcccg ttcctcgatc gggatagcgc gtccggaacg
     1861 catttcccag tcgatggtcg agagcggaat cgccacgtag aaggggagtc gatggcgacg
     1921 ggcgaggacc gccttggtgt aggtgccgat cttgttggcg acttcgccgg tccgggccag
     1981 gacccggtcg ctgcccacga tgacaagatc gatctcgccg cgctgcatca agtgcccggc
     2041 ggcattgtcc gcgatcacct ggtgcgagat gccctgctgg gccagctccc aggccgtaag
     2101 cgatgccccc tggcagcggg gccgcgtttc gtcgcaatag acatggaagg accgaccggc
     2161 cgcctgcgcc gcgtagagcg gggcggtggc gctgcccaca tccacgaagg ccagccagcc
     2221 ggcgttgcaa tgcgtcagca cgcgcgcccc ttccgggatg aggtccaggc catgccgccc
     2281 gatggcttcg cactcccgga cgttcgtgtc ggcgaaggcc tgtgccgccg ccagcgccag
     2341 atcctgccgt tccgcgaccg tgccgccttg cgccatccgg cggcggacct ggtccatggc
     2401 attgaccggg tcgaccgcag tcggtcgggc ggctttgagc gtctcgtaga cggtggcgag
     2461 acaggcttcg aagcgatcga ggttcgcgcc gcgaaacgcg cgggcaccct gggcgagtcc
     2521 gaaggcagcc gtcgccccga tcgctccggc gccccggacc gccatgtcgc gaatggcggc
     2581 ggctgttgcg cggtagtcgg gcgtggcgac cactttgaac cgatgcggca acaaccgttg
     2641 ttcgatcaac cgcaccgtgt tgtggcgagc atcaaagtcg acggtgcggt agtggcgggt
     2701 gcggttgccg acggtgactt tcataatccg ccgtcaccgc atctcgatcc tcagcgcttt
     2761 gactcgggca cgaagcttgc gatagagcgg gtgcttgcgg aaggaggcca ggtcgggatc
     2821 ccgggccagc cagcggaaat cgcgataccc gcggtcgatg gcgagattga gggccgcgta
     2881 agcggcatcg aactggttgg tgagggcgta gctgcaggcg aggttgtaat gggccagcgg
     2941 atcttccggc cgcaggcgga ccaactgcgc atccacgcgc agcccgtcct cgaagcgccc
     3001 gcgccgcgag taatcgtcgg cgaggagcct cagggcgtcg tcatagaacg gatcgcgtcg
     3061 tgtgagtccc tcgaggaacc cgatctcgat gtccaaattg cggcgttcgt cgcggcgcag
     3121 cttctctctc ctggtctcag ccataaaccc gggccaaatc atgccagcca ttgcggcagc
     3181 gtcaagggac agtgaaggag agtgaaggac gtgccgaagc gcgtcctcac gagcttgacg
     3241 atggggcggg cctgggaaac ccttgccttc atgattcgca tgcgatttgt cggtcggctt
     3301 gcttttgcgc tcggtctggc cgcctggctg ccggcggtcc ccggagcggc gctaagggcg
     3361 gcatcttcgc cgtcggcggc aaccgacgct cctggggggc tcagaatctg gtttaccgag
     3421 ccggcgacca actgggagaa gcaggcattt ccgatcggca acgggcgttt gggggcgatg
     3481 gtgttcggag ggaccgggcg tgagcgcatt caactcaacg aggacacgct ctggtcgggc
     3541 ggcccgcgcg acaccaccaa ccccgaggcg ctcaaacacc tgcccgaggt gcgacggctc
     3601 ctgttcgacg ggcggccgga cgaggccgtc aaggtggccc aggagcatct catgggccgg
     3661 cccatgagga tcaaaccgta ccagtcgctg ggtgatctgc gcctcgattt tccggggcac
     3721 gaagcggccg aggactaccg gcgcgaactg gatctcgaaa gggcggttgt gcgcatccag
     3781 taccgggttg gggagattcg gcacacgcgc gaactctttg cgagccatcc cgatcaggtg
     3841 ctcgtgctgc gcctcacctg ctcgaagccg ggcggtttga cattctttgc gacgctggat
     3901 cgcgagcagg atttccggac cagcacgcgt tcgcccgatc gcgtcctgct gcagggacag
     3961 gtggatggcg ggcaggggac cgagtactgc gccgttcttc aagcgcacgc ccagggcggc
     4021 cagatgttcg ccccagcgcg ccgcctcgaa gtgcggaacg cgacttcggt cactttggtt
     4081 ctgggggcgg cgacgagctt tggcgggcgc gacgcgctgc aggttgccac cgacgacgtc
     4141 gaggcggcga tccgcaagcc gtacgccgcc ctgatggcgg cgcacgtgcg cgattaccag
     4201 gcgttgttca accgggtcag cctcgatctg ggcggggcgg acgccgcggt ccagcccacc
     4261 gacgcgcggc tggctgcgat gaagaaaggc gggaacgacc cccatctgct ggccacctac
     4321 tttcaattcg gacgttatct gttgatttcg tgttctcgtc cgggcgatct gcctgcgaat
     4381 ctccaaggtc tttgggctga gggcatgaac ccgccctgga acagcgatta ccatctgaac
     4441 atcaatctcc aaatgaacta ctggcccgcc gagaccacgg ccctttccga atgccaccag
     4501 ccgctgttcg agctgatcga gtcgctgcgc gagtcgggcc ggcgcacggc ccgggtgcac
     4561 tacggcgctc gcggctgggt ggcgcatcac ttgaccgatg tgtgggggtt caccacgccc
     4621 gcggacggcg cgggctgggg attgtggccg atgggcgcgg cgtggctttg ccagcatctg
     4681 tgggagcatt tcgcgtttac gggcgaccgg acgtttctgg ccaggcgggc ttatccgccc
     4741 atgaaagaag ccgcggagtt cttcctcgat ttcctggtcg aggattcgaa agggcggctg
     4801 gtgagcgggc cctcgagttc gccggagaac acctaccgcc tcccgaacgg cacgcagggc
     4861 ggactgtgca tgggaccgtc gatggactcg caaatcatcc gggatctctt ctcgaattgc
     4921 attcgagcct ccgagattct ccggatcgac accgagttcc gcggccgact cgccgccacc
     4981 ctggccaaac tgccgcccac ccagatcgga cgccacggcc agatcatgga atggtccgag
     5041 gattacgatg aacccgagcc ggggcatcgg cacatctccc acctgttcgc cttgcatccg
     5101 ggcagggaga tcacccgcac gggcacgccc gatctcgccc aggcggcccg gaagacgctc
     5161 gaacgacgac tcgcgcacgg cggcggccac accggctgga gccgggcgtg gattctgaac
     5221 ttctgggcgc ggctgggaga cgccgacaag gcgcacgaga atctgctggc gttgctgcgc
     5281 cacagcacgg cgcccaacct gctcgacctg cacccgccct tccagattga cggcaacttc
     5341 ggaggcacgg ccggcctcgc ggagatgctt ctccaaagcc acgccgggga aatcgagttg
     5401 ctgcccgcca aaccgtccgc ctgggccgat gggcatgtca gcggattgcg cgcccggggc
     5461 gggttcgcgg tcgatctcgc gtggcgcggc ggacaattcg agtcggggcg cattgaatcg
     5521 ctcggtggga atgtgtgccg cctccggtgt ccggtcccgg tcaccgtgcg ctcggggtcg
     5581 gcgcgcggcg cggtggtgaa gaccaggccg ctggccaccc aggcgatcga gttcgccaca
     5641 aaacggggcg ggcagtattt tctatcgcgc gatgcacact gacttgcagc cgctcgaagc
     5701 ggccatcggg cggcatcaag cgcggttgga ggcgctgcgc gcggagatgg cgcgttcgat
     5761 cgtggggcag cagacgctga tcgagcggct cctggtcggc ttgcttgcga acggccacgt
     5821 cctgctcgaa ggcgtgcccg gcctggccaa gaccgccacc gtaaatgccc tgggccaatg
     5881 cctgcacctc aagttcgccc gcgtccaatt cactccggac ctcctgccgg gcgatctgat
     5941 tggcaacctg gtcttcaatc cgcgcgacaa cagctaccac acgcgcaagg gcccgatctt
     6001 tgcgaacctg gtcctggcgg acgaaatcaa ccgtgcccca gccaaggtgc agagcgcgct
     6061 gctcgaagcg atgcaggaac ggcaggtcac gatcggcgaa cagagcttcc cgctgccctc
     6121 gccctttctg gtgttggcga ctatgaatcc gatcgagcaa gaggggacat attcgctgcc
     6181 ggaagcgcag gtagaccgct tcatgctcaa ggtcgtggtc acgtatccga ccgaacccga
     6241 ggagcggcag atcatggccc tgatggcccg cacggccccg cgcccgccgc tggcgacggt
     6301 ggtgcgaccc gaggatatcc tggccatgcg cgcgctggtc gatcaggtgt tcgtcgacga
     6361 acaggtgtcg gactatatcg tgcggctggt gttcgccacg cggaatccgt cgccggtggc
     6421 gccgcgcctg gcgcccttca tccggttcgg cgcgtcgccc cgggcctcga tcaacctgtc
     6481 gctggcttcc aaagcgctcg cgttcctgcg cgggcgtccg catgtcacac ccgcggacgt
     6541 caaggcggtc gcggcggacg tcctgcgcca cagggtgctg acgacattcg aggccgatgc
     6601 ggaccggatc acctcggagc acattgtgtc ggagttactc gcccaggtgc cggtgccttg
     6661 aagtggagag tggagagtgc agagttgtga gtttaacggt tctcgttcat ggtcctcgca
     6721 cgcactaaac tgttgaatgc caaagctctc ccgtgagacg cgccccgtca catcgcctct
     6781 cgccgccgct tcggaccaag gagggcgcgt caccggaccg cgatcccacc tcgcacggcg
     6841 cgaccgaaat catgcggcgg gtacgtctgg tcgagttgcg cgcccggcgg ttcgtggaag
     6901 acctgctcgc cggcccctat cgttcggtgt ttcggggtcg ggggctcgaa ttcgatcagg
     6961 tgcgcgccta cgagccggga gatgacgtgc gcagcatcga ctggcacacc acagcccgca
     7021 cgggagcggt tcagatcaaa cagggcatcg aggaacgatc gctgacggtg atgctggcgg
     7081 tggatgtgag cgcctcgggc gatttcgggt cggtgccaca gagcaaacgc gaactggccg
     7141 ctgagttggc ggcggtcctg gccctcagcg cggtcctgaa caacgatcgt gtgggactgc
     7201 tgctcttcac ggacgccgtc gagtgctatc tcgcaccacg caccgggcgc cgccaggccc
     7261 tcgccattgt ctcggccatc ctcacccacg aggcccgttc accccgcacc tcgctgcgcc
     7321 aggccctcca aatcctcggc aacgcgctgc aaaggcgcgc gacggtgttt gtgctttcgg
     7381 atttctgtga cgagggattc gagcgcgcgc tgcgcctggc ggcgcggcgc cacgacgtgg
     7441 ttgccatgtg cgtccatgac ccggtcgagg cgcgactgcc cgaggtcggg tggctcgtgt
     7501 gcgaggatgc ggaaacg
//
LOCUS       DAOQRO010000154         7508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9491_length_7508_cov_4.567288, whole genome shotgun sequence.
ACCESSION   DAOQRO010000154 DAOQRO010000000
VERSION     DAOQRO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9491_length_7508_cov_4.567288"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKK20_07170"
     CDS             <1..812
                     /locus_tag="PKK20_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99704.1"
                     /translation="KAHLLLGAIAAEDKRYAEAADAYAKAILLDADLEPAYYELAAMR
                     LVLGEPDQALATLTQARTRFGANFLVDFYSGLALAVRKEYAEALKHYTSAEIQARASE
                     PSRLNHVFYFQLGSLYERMANAAFAQKRPEEGERHFTEAEKNLRRSLELSPDNAEVLN
                     YLGYMWAERGVNLEEARRMIEKAVALEPESAAILDSLAWVLFQLKQPQAALEPMLKAI
                     KLSPKPDATLFDHLGDIYNAVGRVTEAREAWRESLDVEPNESVRSKLGAEP"
     gene            1055..2029
                     /locus_tag="PKK20_07175"
     CDS             1055..2029
                     /locus_tag="PKK20_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU99705.1"
                     /translation="MIKVHNLVKAFGSKLAVNDVSFTVERGEVLGFLGPNGAGKSTTM
                     RMVTGFIPPTSGRISVGGFDLVNNPIPAKRLIGYLPENAPAYTDMTVYGFLNFTAELR
                     GLSGEFRKKAVHRAVEMCFLDSVLYQSVDTLSKGYRHRTCFAQSIIHDPDILIMDEPT
                     DGLDPNQKHEVRTLIRRMGEKKAIVFSTHILEEVDAACTRAIVIDRGKIVANGTPREL
                     RQKSDLAGAVSLRVAGVPGAVLRERLQQLPTVKRCTIVREEPGAAWARAYGAKGTRDN
                     ELNQSLYELAVREQWKLIELKAEEGRLEEVFRAITLPDTPATSKEQAQ"
     gene            2095..2829
                     /locus_tag="PKK20_07180"
     CDS             2095..2829
                     /locus_tag="PKK20_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNU99706.1"
                     /translation="MRHIITIAKRELTSYFASPVAYVFIVFFLLLCGFFTFMVGRFFQ
                     RGEASLIAFFIWHPWLYLFLVPAVGMRTWSEERRLGTIELLLTMPITPWQAIVGKFLA
                     SWLFLAIALALTFPLVLTVGYLGSPDYGVIFCGYLGSLLMAGAYLAISCMTSALTRNQ
                     VISFILSVVTCLFLILAGWPPVTDMLVQWAPNWLVEGVAAFSVMPHFESFQRGVIDSR
                     DLVYFLSVIAFSLFTTGVIIRSHRAG"
     gene            2873..4825
                     /locus_tag="PKK20_07185"
     CDS             2873..4825
                     /locus_tag="PKK20_07185"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNU99707.1"
                     /translation="MNTNKHKWEPLLYSGVGVGAMFLILVAVGIIAGTAKVRLDLTAD
                     RVYTLSDGTKKTLAKLDTPVTINFYCTQSENRMPVQLKTYARRVEDLLDEYRQRSNGK
                     IEIKKFDPQPDSDAEDSARLDGIEGQSIGEGGIIGLGEKVYLGLSVTCLDQKVALTFL
                     DPSRERLLEYDLTRAIAQVVNPQKQNLGVMSGLPIFGQMMNPMMAQMGGGQQEPWIVL
                     NELKRDFNVKQIDMSAEKIDDDINVLLVVHPSNISDKAQYTLDQFVLRGGKLIALLDP
                     LSVFDSRNSMNPQNMMQRAASGGSDLPKLLAAWGIKFEGDKVIADKNYVTMVRRGAGG
                     TPAPELTWLSLTADAINQNEIVTSQIDSLLLAGAGIFSGTPIEGLNQKVLVKTSVNSD
                     GVDKMMAQFGADTSKDFKPSGKESPLALMLTGKFKTAFPDGPPGGAAADAAGDDKEKA
                     EGGDAADKAGSLKESQKEGAVLLVGDTDFIYDQFCVQVQNFFGQKLVSPFNGNLGFLQ
                     NVIEQFMGDENLIQVRSRAVQNRPFTRVRKIQAEAEERYTEKIKNLKSDLEEAQQKIN
                     ELQRNKDQNQRFILPPEVQAEIKRFREKEVQTKRDLKEVRRQLRKDIDALETRLKWFN
                     IAAVPMLVTAAWIGFAVVKRKKTAAK"
     gene            4822..6309
                     /locus_tag="PKK20_07190"
     CDS             4822..6309
                     /locus_tag="PKK20_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNU99708.1"
                     /translation="MNRKQLILVLLAVVVLGGLGLWLHNRDRSSYKTSQGQMGQKVLG
                     EFDLNTVAAISIKHATNELHLVRTNNLWTVLQRGGYPANFSEISETLRKLWELKVVQP
                     IRIGPSQLGRLELTSSEDTNSATLVTLVDKEGKPLRKILLGKKHMRQSSSPSPFGGDD
                     GWPDGRYVMLEDKPDSASLVSETFSNLEPKPEQWLNKDFFKVEKVKSIAVSYPVATNS
                     WTLTRETETGQWTLADAQPGEQLDNSKTSSLANALSWPSFNDVVLDPQPDTTGLDHPT
                     VAVLETFDNFTYTVKAGKKSDEDAYYMTVTVAAEIPQTRTPGEDEKQEDKDRLDKEFK
                     EKTDKFNEKLKKEKALENWTFLVSKWSLDSLLKERHTLMADKKAEEPGETTQPAEPAP
                     EPLVPDLTPVPPSPAPSSTDVIKPNLPTPPVPPAPPPPPAQTPKPETPPAPPATETPP
                     TPAPQAPETPPSTETPPAPETPPSTETPPAPETPPGRAADASNTP"
     gene            complement(6512..>7508)
                     /locus_tag="PKK20_07195"
     CDS             complement(6512..>7508)
                     /locus_tag="PKK20_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HNU99709.1"
                     /translation="VVKHEGRDPRLFWHAPTGRWCMAVYDEFRGGRWIAFYSSPDLKA
                     WTFHSRIEGFFECPDIFELPVDAQAGRGADAGNAASKWVLTAASSAYRLGQFDGRVFT
                     PETPMLPGHRGESFYAAQTYSDMPDGRRVQIGWGQMATPGMPFNQMMTFPCELSLRTT
                     DDGVRLCWQPVRELERLRARKHTFDPRALEPGDNPLANLNIDLHETRIEANPGSASAL
                     RLSVGGIDVIYDAVARELICKKHRAPLRLRDGRMSLRVLTDRTSIEIFGNEGEVYMPI
                     YRPPELSGTRSVGLLAEGGVARVLSLEVNELKSIWLQAQEGGAGSAAAASTPVMR"
BASE COUNT         1548 a         2572 c         2135 g         1253 t
ORIGIN      
        1 ccaaggccca cctgctcctg ggtgccatcg ccgcggagga caagcggtat gcggaagccg
       61 cggatgccta tgcaaaggcg attctgctcg atgcggatct ggaaccggcc tactacgagt
      121 tggccgccat gcggctggtg ctgggcgaac ccgaccaagc cctggccact ctcacgcagg
      181 cccggaccag gttcggcgcg aacttcctgg tcgacttcta ctcgggattg gcgctggccg
      241 tccgcaagga atacgccgag gcactcaagc actacacctc cgcggaaatc caggctcggg
      301 catcggagcc ttcgcgcctc aatcacgttt tttacttcca gctcgggtcg ctctacgagc
      361 gcatggccaa cgcggccttt gcccagaagc ggcccgagga aggcgagcgc cacttcaccg
      421 aagccgagaa gaacctgcgg cgttcgctcg aactgtcgcc ggacaacgcc gaagtgctca
      481 attacctcgg gtacatgtgg gccgagcgcg gcgtgaacct cgaggaggcg cgccggatga
      541 tcgagaaggc cgtggcgctc gaaccggaga gcgccgccat tctcgacagt ctggcctggg
      601 ttctctttca actcaaacaa ccccaggccg ccctcgaacc gatgctcaag gccatcaagc
      661 tttcacccaa accggatgcc acgctgttcg atcatctggg agacatctac aacgccgtgg
      721 gccgagtgac ggaggcccgg gaggcctggc gcgagtccct cgacgtcgag cccaacgaaa
      781 gcgtccgatc caaactggga gccgagccgt gatagggccg accgcgagcc aaggatgcgc
      841 cccgaccggc ctcgggcagg cacgggcatc ggacacccga tttgaacatt ttgggtcggg
      901 atggtctaaa acgcttgaaa tgaggcttgg cacttgacac cgggtgtaaa aaaatgtctc
      961 gcgcaatcgg gaattgcgcg ggcgggggtg ttggtgcggg tccgcccggc ttgagcgcgc
     1021 gcatggcgcg aacttgtgat gtggagagtg atgcatgatt aaagtacaca acctggtcaa
     1081 ggccttcggc tcgaagctgg cggtcaacga cgtgtcgttt accgtcgaac ggggcgaggt
     1141 gcttggattt ctcggtccca acggcgcggg caagtcgacc accatgcgca tggtgacggg
     1201 cttcatcccc ccaacctccg gccgcatcag cgtcggcggc ttcgacttgg tcaacaaccc
     1261 gatccccgcc aagcgcctga tcggttatct gccggagaat gcccccgcat acaccgacat
     1321 gacagtctac ggctttctca atttcacggc ggagttgcgc gggctcagtg gcgagttccg
     1381 caagaaggcc gtgcaccgcg ccgtcgagat gtgcttcctc gactcggtgc tctaccagag
     1441 cgtggacacg ctctcgaaag ggtaccgtca ccggacctgc ttcgcccaat ccatcattca
     1501 tgacccggac attctgatca tggacgagcc gaccgacggc ctggacccaa atcagaagca
     1561 cgaggtgcgc accctcatcc ggcgcatggg cgagaaaaag gcgatcgtct tctcgaccca
     1621 cattctcgag gaggtcgacg ccgcatgcac acgggccatc gtcatcgacc gcggcaagat
     1681 cgtcgccaac ggcaccccgc gcgagctgcg gcagaagtcc gacctcgccg gcgcggtgtc
     1741 cctccgcgtg gccggcgtcc ccggcgcggt cctgcgcgag cgcctgcagc aactgcccac
     1801 ggtcaaacgc tgcaccatcg tccgggagga gccgggcgcc gcatgggcgc gcgcctacgg
     1861 ggccaaaggg acgcgcgaca acgaactgaa ccagagtctt tacgaactgg cggtgcgcga
     1921 gcagtggaag ctcatcgagt tgaaagctga ggagggacgc ctcgaggaag tcttccgggc
     1981 catcaccctt ccggacacgc cagccaccag caaggaacaa gcccaatgag cacagacgca
     2041 ctcacgatga atccggccgc cggcctgtcg gacgcccccg cgcacaccag cgccgtgcgg
     2101 catatcatca ccatcgccaa acgcgagctg acgtcgtatt tcgcctcccc cgtggcgtat
     2161 gtcttcatcg tgttcttcct gcttctttgc gggtttttca cgttcatggt cggacgattc
     2221 ttccaacgcg gcgaggcctc gctgattgcc ttcttcatat ggcatccgtg gctctacctg
     2281 tttctggtgc ccgcagtggg gatgcgcacc tggtccgagg aaaggcgcct ggggacgatc
     2341 gaactgctcc tgaccatgcc gattacgccc tggcaggcca ttgtgggcaa attcctcgcc
     2401 tcctggctct tcctggccat tgcgttggcc ctgacctttc cgctggtcct gacggtcggg
     2461 tacttgggca gccccgacta cggcgtcatc ttctgcggct acctcggcag cctgctcatg
     2521 gcgggcgcct acctggccat cagttgcatg acctcggcgc tcacccgcaa ccaggtcatc
     2581 agcttcatcc tctcggttgt gacctgcctc ttcctcatcc tggccggctg gccgcccgtc
     2641 acagacatgc tcgtgcaatg ggctcccaac tggctcgtcg agggagtcgc cgccttcagc
     2701 gtgatgcccc acttcgagag tttccagcgg ggcgtgatcg actcgcgcga cctggtgtac
     2761 ttcctgtcgg tgatcgcttt cagcttgttc acgaccggag tgatcatccg cagtcatcgg
     2821 gccggctgac ccggcaaccg tctttaccat tcgcaaacgc cgtactcgca tcatgaacac
     2881 caacaaacac aaatgggagc cgctcctcta ctccggcgtg ggcgtgggcg cgatgttcct
     2941 catcctggtg gccgtcggca tcatcgccgg aaccgccaag gtccgcctcg atctgaccgc
     3001 ggatcgcgtg tacacgcttt cggacggcac caagaagacc ctggccaaac tcgacacgcc
     3061 ggtcacgatc aacttctact gcacccagtc cgaaaaccga atgcctgttc agttgaaaac
     3121 gtacgccagg cgggtcgaag acctgctgga cgagtaccgc cagcgctcga acggcaagat
     3181 cgaaatcaag aagttcgatc cccagcccga ttcggacgcc gaagactcgg cccggctcga
     3241 tggcatcgag ggccagagca tcggcgaagg cggcatcatc ggcctcggcg agaaggtcta
     3301 cctcggcctc tcggtcacgt gtctcgatca aaaggtggct ctgactttcc tggatccctc
     3361 ccgcgaacgg ctcctggaat atgacctcac tcgagccatc gcccaggtcg tcaaccccca
     3421 aaagcagaac ctcggcgtca tgagcggtct gcccatcttc ggccagatga tgaatccgat
     3481 gatggcccag atgggcggag gccagcagga accgtggatc gtcctcaacg aactgaaacg
     3541 cgacttcaac gtcaagcaga tcgacatgag cgcggagaag atcgatgacg acatcaacgt
     3601 cctcctcgtc gtccacccgt ccaacatctc cgacaaggcc cagtataccc tggatcagtt
     3661 cgtgttgcgc ggcggcaaac tcattgccct gctcgatccg ctgtcggtgt tcgactcgcg
     3721 caactcgatg aatccccaga acatgatgca gcgcgcggct tcgggcggtt cagaccttcc
     3781 caaactcctc gccgcctggg gcatcaagtt cgagggcgac aaggtcatcg cggacaagaa
     3841 ctacgtcacc atggtgcggc gcggcgccgg cggaaccccc gcgcccgaac tgacctggct
     3901 ctccctcacg gccgacgcga tcaatcagaa cgagatcgtt acaagccaga tcgacagcct
     3961 cctgctcgcc ggagccggca tcttcagcgg cacgccaatc gaaggcctca accaaaaggt
     4021 cctcgtcaag acctccgtca actcggatgg cgtcgataaa atgatggccc agttcggagc
     4081 cgacacgtcg aaggacttca aaccgtccgg caaggaatcc ccactggctc tcatgctgac
     4141 cggcaaattc aaaacggcct ttcccgacgg cccgcccggc ggcgccgccg cggacgccgc
     4201 gggcgatgac aaggagaaag ccgagggcgg cgatgccgcg gacaaagccg gctctctcaa
     4261 ggagtcgcag aaggagggcg cggtgttgct cgtcggcgac accgatttca tctacgacca
     4321 attctgcgtg caggttcaga atttcttcgg ccagaagctc gtctcgccct tcaacgggaa
     4381 tctgggcttc ctccaaaacg tcatcgaaca gttcatgggc gatgagaacc tgattcaggt
     4441 ccgcagccgg gcggtgcaga accggccctt cacgcgggtg cgcaagatcc aggccgaagc
     4501 tgaagagcgc tacaccgaga agatcaagaa tctgaaaagc gacctcgaag aggcgcaaca
     4561 gaagatcaac gaactccagc gcaacaaaga ccagaaccaa cgtttcatcc tgccgcccga
     4621 ggtgcaggcg gagatcaaac ggttccggga aaaggaagtc caaaccaagc gcgacttgaa
     4681 ggaggtccgc aggcagttgc gcaaggacat tgacgcgctc gagacccggt tgaaatggtt
     4741 caatattgcc gccgtgccga tgctcgtgac ggcggcatgg atcggcttcg ccgtggtgaa
     4801 acgcaagaaa accgctgcca aatgaatcgc aaacaactca tcctcgtcct gctcgcggtg
     4861 gtggtcctgg gcggcctcgg actgtggctg cacaaccgcg acagatcctc ctacaagacc
     4921 agccagggcc agatgggcca aaaagtcctc ggcgagttcg acctaaacac cgtggctgcc
     4981 atcagcatca aacacgccac caacgagctg catctcgtcc gaacaaacaa cctgtggacc
     5041 gtcctgcagc gcggcggcta cccggccaac ttcagcgaga tcagcgaaac cctccgcaaa
     5101 ctctgggaac tcaaggtcgt ccagccaatc cgcatcggcc catcccaact cggtcgcctc
     5161 gaattgacat cgtccgagga taccaacagc gccacgctcg tcacgctggt cgacaaggaa
     5221 ggcaagcccc tgcgcaaaat tctcctgggc aagaaacaca tgcgccaatc ctccagcccc
     5281 tcgcccttcg gaggcgacga cggctggccc gacggacgct acgtcatgct cgaagacaaa
     5341 cccgatagcg ccagcctcgt gtccgagacc ttcagcaacc tcgaacccaa gcccgagcag
     5401 tggctcaaca aggacttctt caaggtcgag aaggtcaagt cgatcgcagt cagctacccg
     5461 gtcgccacca actcgtggac cctgacccgc gagacagaga ccggccagtg gaccctcgcg
     5521 gacgcccaac cgggcgaaca actcgacaat tcgaaaacat cgagcctggc caacgccctc
     5581 tcctggccca gcttcaacga cgtcgtgctc gatccgcaac ccgacacgac cggcctcgat
     5641 caccccaccg tcgccgttct cgagaccttc gacaacttca cctacaccgt gaaagcgggc
     5701 aagaagagcg acgaggacgc ctactacatg accgtgactg tggccgccga aatcccccag
     5761 acacgcacgc ccggagagga tgagaaacag gaggacaagg atagactcga caaggagttt
     5821 aaagaaaaga ccgacaagtt caacgagaag ctcaagaagg aaaaggccct cgagaattgg
     5881 accttcctcg tctcgaaatg gtccctcgac tccctgctca aggaacgaca caccctgatg
     5941 gcggacaaga aggcggagga acccggggaa accacccagc cagccgagcc cgctcccgaa
     6001 cccctggtgc cggacctcac acccgtccca ccctcccctg ccccatcctc gacggacgtc
     6061 atcaagccga atctgcccac gccgccggtc ccccctgcac cgccgccgcc gccggctcaa
     6121 acacccaaac cggagacgcc gcccgccccg cctgcaactg agacgccgcc cactcccgcg
     6181 ccacaggcgc ccgaaacgcc gccatccact gagacaccgc cggcgcccga gacgccgcca
     6241 tccactgaga caccaccggc gcccgagacg ccgcctggca gagcagcgga tgcctcgaat
     6301 acgccatagc ctgcagatct gtctccctgt tccagcttga gattctctgc ccggcagcct
     6361 gtcggtcttt gccgacagcc tccactggag tccctgccag cctgtctcgc ctccgatggg
     6421 cgttttacca ggggcggttt cctcgggaga accgtcctca atcgcattga aatgcgcggg
     6481 ctagctcagt ttttccacag acccttaatc tctacctcat caccggagtg gacgccgccg
     6541 ccgcggagcc cgctccgccc tcctgcgcct gaagccagat cgacttcagt tcgttcacct
     6601 cgagcgacag cactcgagcc acgccgccct cggccagcag tccgacggac ctggtcccgc
     6661 tcagctcggg cgggcggtaa atcggcatgt acacctcgcc ttcgttccca aaaatctcga
     6721 tcgacgtgcg gtccgtcagg acgcgcagcg acatccgccc gtcgcgcagg cgcaacggcg
     6781 cgcggtgctt cttgcagatc aactcccgcg ccacagcgtc gtagatcaca tcgatgccgc
     6841 caaccgacag cctgagagcg gacgcgctgc cgggattcgc ctcgatccgc gtctcatgca
     6901 gatcgatgtt caggtttgcc agcgggttgt cacccggctc gagcgcccgc ggatcgaacg
     6961 tgtgcttgcg cgcgcggagc cgttcgagtt cccggactgg ttgccagcaa aggcgcaccc
     7021 cgtcgtccgt cgtccgcaaa ctcagttcgc acgggaacgt catcatctgg ttgaacggca
     7081 tccccggcgt ggccatctgg ccccagccaa tctgcacccg ccgtccatct ggcatgtcgc
     7141 tgtacgtctg cgcggcataa aacgactcgc cccggtgccc cggcagcatc ggcgtctcgg
     7201 gcgtaaacac cctgccatcg aattgcccca accgatacgc gctgctcgcc gcggtcagaa
     7261 cccacttcga agccgcgttg ccggcgtccg ccccccgccc cgcttgcgcg tcgaccggca
     7321 gttcgaaaat gtctgggcac tcgaagaatc cctcgatccg gctgtgaaac gtccacgctt
     7381 tgagatcggg cgagctgtaa aaggcaatcc agcgtccgcc ccggaactcg tcgtagaccg
     7441 ccatgcacca gcgcccggtc ggggcgtgcc agaagagccg gggatcgcgt ccctcgtgtt
     7501 tgacgacc
//
LOCUS       DAOQRO010000155         7475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9558_length_7475_cov_4.690836, whole genome shotgun sequence.
ACCESSION   DAOQRO010000155 DAOQRO010000000
VERSION     DAOQRO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_7475_cov_4.690836"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PKK20_07200"
     CDS             complement(<1..1332)
                     /locus_tag="PKK20_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU99710.1"
                     /translation="MKKWIVWILVLGVLGFLGYGPWKAWRAKAREADGNKRPSTASVI
                     QTNISFAVNAAGEISPAEQVSVRPEINGKIEVLPVDIGDQVEKDSLLFKLDDKELQQQ
                     RASNLTAIERARLELAKAERDYKRADEMLADHLISQELHDNTQTTFELAKNSLERAQK
                     ELAIIEERLTKTEVRAPFDCTVLTRPVSIGQAVSGSGGFNSGTEVLSIADLNSMVINA
                     HVNQADVPRLGVSQTVEVSVEAVPGLLVTGVVERIAPQATIRNNIKGFAARILLKNVD
                     RRVRPGMTANVKIPVASVENVLAVPLAAVFTELNPETGQMERFAYVQNPTGYERRPVR
                     IGVSDFFYAEVQDGLAPGETVSLELPKEQRDKPSKNLAGAPFAPGRVAAAARATGAPP
                     ATASAPRPAEGAGAGGVAAPAAGASNAPPAAKIETTATSGTTATSGTTATSG"
     gene            complement(1412..2128)
                     /locus_tag="PKK20_07205"
     CDS             complement(1412..2128)
                     /locus_tag="PKK20_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HNU99711.1"
                     /translation="MNSLNVIAPREAGKMRTFARRSISSGLTLALCLGLTGCSALKPV
                     EVEERHFVLSPLPGEAPAAAGSVSARGRAVGVRPLRVAAYLLRDAFVVRRGRHEIQYL
                     ESMRWAERVDRGVQRVFEADLGMLLPDAVIRSTSWRPGDVAVEADIQLEQFDVDSAGN
                     GVLEAAWRIVAHDREGAVRCGRSRHTASGPQPSADPDGAVGTLSLLIGALSRDVAGAV
                     LGQTRIEPEGAGRPGRADHP"
     gene            complement(2197..3204)
                     /locus_tag="PKK20_07210"
     CDS             complement(2197..3204)
                     /locus_tag="PKK20_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNU99712.1"
                     /translation="MSTRVKPATIGVFVVCGLALAVAGVVMSSSSRWFSRTEECIAYF
                     DGSLNGLDNGAPVKYRGVPIGYVKRVMARYNQAPADNAMPVLMEIQERLIVDRLGPKA
                     AARVPSGLSAWLTENTRATLEAESLLTGVLYVNIEAKSNPAPPVFHQLDAIYPEIPTE
                     PTRIQQIIAQLTKVDLAGLADRLTALITRLDSKLGEIKTAEISDGLTNLIASLDGFIN
                     SPELSNTLASARATLDEYRSVGSRVGARIDPLADGLHETLAQSQKTLADIRGAAANLE
                     MVLGGNAPLWHDLGLALDQLAAAAQSISGLADFLKAHPNALISGRRTPRGSLDHGRAA
                     D"
     gene            complement(3201..4010)
                     /locus_tag="PKK20_07215"
     CDS             complement(3201..4010)
                     /locus_tag="PKK20_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU99713.1"
                     /translation="MPARVDKIEIRDVTLAYGSFVVLRDIRARIRSGAVFVIMGGSGC
                     GKSTLLQALIGLRAPTDGEVLYDGIPFWGSGPEARQERLRSFGVLFQNGALWSSMTLA
                     ENIGLVLGEFTDLTPPEIRDLSRFKLSLVGLRGFEDFYPSQVSGGMAKRAALARALAL
                     DPDILFLDEPSAGLDPITSRKLDELILQLRDSLGATFVVVTHELASIFAIADECLFLD
                     GSTRTMRAQGSPRALLERADDPGVRAFLTRGASGMEPTDPAHAALGNLTKE"
     gene            complement(4040..5206)
                     /locus_tag="PKK20_07220"
     CDS             complement(4040..5206)
                     /locus_tag="PKK20_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU99714.1"
                     /translation="MKSHRHSALGADGPSPARLDVEAAGEWSLVVRLSGDWLLSSDVP
                     AFTEIERSIRAGSARRLGFETAALGRWNSALMVFLSKCTDLCRDRGIEMDTSGLPPGA
                     ANLLRLARTAADKRAPASGARSATWLERVGEGVLAFAGGAGGFLTFLGECVVALFRLL
                     RGRAQFRWSDTFLVMQECGPQALGIVALINFIVGLILAFVGTLQLARFGATIYSADLV
                     AIATVREMACLMTGVIMCGRTGAAFAAQLGTMRVNQEIDAFQACGISPIEFLVLPRLM
                     ALILMMPLLCVFAAAIAIAGGFAVSTLMLDVTPTVYVRRTIGAVTLVSFLLGLGKGTI
                     FGVLVALTGCHRGIQCGTNAAAVGRATTTAVVTGITAIIVSDGVFAVVCSALNI"
     gene            complement(5219..>7475)
                     /locus_tag="PKK20_07225"
     CDS             complement(5219..>7475)
                     /locus_tag="PKK20_07225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNU99715.1"
                     /translation="WINTPDSGSAVALDILCDIMQNASLPGSELEKEMEVIRREMDMC
                     HDDPGRRASQRLFETAYTHSPYRYAVIGHPDIFSRLQRDDVVAYYREKYAPNNLFVVV
                     VGAVEADAVFSRVEAGFAASKARPLPVAPFPIEPRQVAPREVIEEAPIELGHAHWSWH
                     VPDVRHPDVPLLDVLATLLGSGRSSRLFQAVRERQGLVTSVDAWTYSPGKAGLLGVSA
                     TVEGAKFAAAREAVLSEIERVRAEEVSEVELAKAVKQFTAAFLATRKTMQGQAQDLGG
                     NWIATGDLSFSERYLRAVKGATPSEVRRVARAYLTTENRSQYALLPACARSAAAVAIV
                     RRETEPVRLFTLGNGLRLLVKEDRRLPFVEIRMVLGGGVLAETPARNGITQLFTRMLM
                     QGTELRDAVRVASEIESVGGHLDTYGGHNSFGITAEVLREDLALGLELTADVLLRPAL
                     PLEALERERQNQIAAIRARGDHVLQSAFRAMRRAMFGVEGYGLDLLGEEESVGRLGVE
                     PLRDFHRRLVAPRNAVLAVYGDIDAEEVQARAEAVLGLWTGEGPGPSGARGAPDTLGT
                     GPVRVEEIRDKKQAVVVAAFPGGTLFDADRFALEVLQEVCSDLGSRLFLRIREKLGLA
                     YYVGAQNFLGLAPGYFAFYAGTAPDAAARVEAELLDEAALLRAEGVSEDELDRAKAKI
                     IGQKKIARQDVGGCALAAALDELFGLGYAYSDTEDAQYEAVTRDQVQAAAVRHLTPDR
                     HVIAVVKPNRA"
BASE COUNT         1344 a         2642 c         2296 g         1193 t
ORIGIN      
        1 cccgctcgtg gcagtggtcc cgctcgtggc agtggtcccg ctcgtggcag tggtctcgat
       61 cttggccgcc ggcggcgcgt tcgaagctcc tgctgccggc gcagcaacac caccagcccc
      121 cgccccctcg gccggacgcg gagcggaagc cgtcgccggc ggggcgccgg tggcgcgggc
      181 ggcagcggcg acccgcccgg gagcgaacgg cgcgccggcg aggtttttcg acggtttgtc
      241 gcgctgttcc ttcggaagct ccagggagac ggtctcaccc ggcgcgaggc cgtcctggac
      301 ctcggcatag aagaaatccg aaacgccgat tcgcacgggg cgccgttcgt agccggtcgg
      361 gttctgcacg taggcgaaac gttccatctg gccggtctcg gggttgagtt ccgtgaacac
      421 cgcggcgaga ggaaccgcga gcacgttctc gaccgaggcg acgggaatct tgacgtttgc
      481 cgtcatgccc gggcgcaccc gtcggtcgac gttcttgagc aggatgcggg cggcgaagcc
      541 tttgatgttg ttccggatgg tcgcctgcgg agcgatgcgc tcgaccacgc ccgtcaccag
      601 aagaccgggc acggcctcga cggagacctc gaccgtttgg ctgacaccca ggcggggcac
      661 gtccgcctgg ttgacatgcg cattgatcac catcgagttc aaatcggcaa tggacaggac
      721 ctccgttccg ctgttgaacc cgcccgaccc ggacaccgcc tgtccgatcg agacgggtcg
      781 tgtgagcacg gtgcagtcga agggcgcccg cacctccgtc ttcgtcagac gttcctcgat
      841 gatggccagt tccttctggg cgcgttcgag cgagttcttg gcgagttcga acgttgtctg
      901 cgtgttgtca tgcagttcct gggaaatcag gtgatcggcg agcatctcat ccgcgcgttt
      961 gtagtcgcgc tccgctttgg cgagctcgag tctggcccgc tcgatggcgg tgaggttcga
     1021 cgcgcgttgc tgctgcagtt ccttgtcgtc cagtttgaac aaaagcgagt ccttctccac
     1081 ctgatcgcca atgtccaccg gcaacacctc gatcttcccg ttgatttccg gacgcacgga
     1141 cacctgctca gcggggctga tctcacccgc ggcgttcacg gcgaagctga tgttggtctg
     1201 aatgaccgag gcggtgctcg gccgtttgtt cccgtcggcc tcccgcgctt tggcgcgcca
     1261 cgccttccac ggcccgtagc ccaggaaacc gagcacgccc agcacaagta tccaaacgat
     1321 ccacttcttc atgacaatca gagcgtcaac actagtctgc cgctgccgcg atgcaaatgc
     1381 tctttgtaga cgagggccgc ggcggacgga ttcaggggtg atccgctcgc ccggggcgcc
     1441 ccgcaccctc cggttcgatc cgggtctggc cgagcacagc ccccgccaca tcacggctca
     1501 gagcgccaat caacaggctc aatgtcccca ccgcgccgtc cggatcggca gatggctgcg
     1561 gtcccgatgc cgtatggcgg ctccgaccac accgcacggc gccctcccga tcgtgcgcga
     1621 cgatgcgcca ggcggcctcc aacacgccgt tgccggccga gtccacatcg aactgctcca
     1681 actggatgtc cgcctcgacc gcgacatcgc cgggcctcca ggatgttgag cgaatcacgg
     1741 catcgggaag caacatgccc aggtccgcct cgaacacgcg ctgcacccct ctgtccacgc
     1801 gttcggccca gcgcatcgac tcgaggtact ggatttcatg gcggccccga cgcacaacga
     1861 acgcatcccg caacaggtag gccgcgacgc gcagcggcct cacacccacc gcccgacccc
     1921 gagccgacac ggatccggcc gcagccggcg cctcgcccgg caaggggctc agaacgaagt
     1981 gccgttcctc gacctcgacc ggcttgaggg ctgagcagcc ggtcaatcca agacagagcg
     2041 ccagggtcag cccggacgag atcgaacggc gtgcgaacgt gcgcatcttt cccgcttcac
     2101 ggggcgcgat gacattcaag gaattcatgt gcagggggac caaaggttct cggagggacc
     2161 ggattcggag gggcgagctc cgcgttccat ccacggtcaa tccgcggccc tcccgtgatc
     2221 gagagagcct cgaggggtgc ggcgcccgct gatgagggcg ttcggatggg ccttgaggaa
     2281 gtcggccagc ccggaaatgg actgggctgc ggccgccagt tggtcgagcg cgaggccaag
     2341 gtcatgccag agcggggcgt tgccgcccag gaccatctcg agattcgcgg cggcgcctcg
     2401 gatatcggcc agcgttttct gcgactgtgc gagggtctca tgaagcccat ccgccaacgg
     2461 gtcaatccgg gcgcccaccc ggcttccgac agaccggtat tcgtccagag tcgcacgcgc
     2521 cgatgccagg gtgttcgaaa gttccggcga attgatgaag ccatcgagcg acgcgatcag
     2581 attggtcagt ccatcgctga tctccgcggt cttgatctca cccagcttgc tgtccaggcg
     2641 agtgatgagg gcggtgaggc gatcggccag gccggccaga tcgacctttg tcaactgggc
     2701 gatgatttgc tggatgcggg tcggttcggt tgggatctcc gggtagatcg catcaagttg
     2761 gtggaacacg ggcggggcag gatttgactt ggcttcgata ttcacgtaga gcacgccggt
     2821 caggaggctc tcagcctcga gcgtggcccg ggtattctcc gtcaaccacg cagaaagtcc
     2881 cgacgggaca cgcgcggcgg ccttcgggcc gaggcggtcg acaatcagcc gctcttggat
     2941 ttccatgagc acaggcatgg cgttgtccgc cggcgcctgg ttgtaccgcg ccatcacccg
     3001 tttgacgtat ccgatcggca caccgcggta cttgaccggc gccccgttgt ccagtccgtt
     3061 gagcgagccg tcgaaatagg cgatgcattc ctcggtgcgc gaaaaccatc tcgaggagct
     3121 ggacatgacc acccccgcca ccgcaagcgc caggccgcag acgacgaaga ccccaatggt
     3181 tgcaggcttg actcgagtgc tcattccttc gtgagattgc ccaacgccgc gtgcgccggg
     3241 tcggttggct ccatgccgga cgcgccgcgc gtgagaaagg cgcgcacccc cggatcgtcc
     3301 gccctctcca acaacgcgcg cggcgatccc tgcgcgcgca tcgtccgggt ggacccgtcc
     3361 agaaaaaggc attcgtcggc gatggcgaag atgctggcga gttcgtgtgt cacgaccaca
     3421 aaggtcgccc ccaggctgtc gcgcaactga agaatcagct cgtccagctt ccgagacgtg
     3481 atcggatcga gccccgcgga gggctcgtcg agaaagagga tgtccggatc caaagcgagg
     3541 gcgcgcgcca gtgcggcgcg tttggccatg ccgccggaca cttgagacgg ataaaagtcc
     3601 tcgaagccgc gcagccccac cagggacaat ttgaaccggg acaggtcccg gatttcgggg
     3661 ggcgtcaggt cggtgaactc gcccagcacc agcccgatgt tctcggccaa tgtcatcgaa
     3721 ctccagaggg cgccattctg gaacaacacg ccaaaggacc gcagccgctc ctgccgggct
     3781 tcaggcccgg agccccagaa cgggatgccg tcgtagagaa cctcgccgtc cgtcggcgcg
     3841 cgcagcccga tcagtgcctg caagagcgtg ctcttgccgc agccgctgcc ccccatgatc
     3901 acgaacaccg cacccgaccg aatccgcgcc cggatgtcgc gcagcaccac aaacgaaccg
     3961 taggcgagcg tcacatcccg gatctcgatc ttgtcgacgc gcgcgggcat gttcggatgc
     4021 gatgtcacgg gacgcgaggc tagatgttga gggcgctgca gaccaccgca aacacgccgt
     4081 ccgacacaat aatcgcagtg atgccggtca ccacggccgt tgtggtcgcc cgaccgaccg
     4141 cggccgcatt ggtcccgcac tgaatgccgc ggtggcatcc ggtcaacgcc accagcacgc
     4201 cgaagatcgt ccccttccca aggcccagca gaaacgagac gagagtgacg gcgccgattg
     4261 tccgacgcac ataaacggtc ggcgtcacgt ccagcatcag ggtcgatacc gcaaagccgc
     4321 cggcaatcgc aattgcagcg gcgaacacgc acagcagcgg catcatcagg atcaaagcca
     4381 tcagccgcgg caggaccaga aactcgatcg gcgaaattcc gcaggcctga aacgcgtcga
     4441 tctcctgatt cacccgcatg gtgcccaact gcgcggcgaa ggccgcgccg gtgcgcccgc
     4501 acatgatgac gccggtcatc aggcatgcca tctcacgaac cgtcgcaatg gccacgagat
     4561 cggcgctgta aatcgtggcg ccgaaccgcg ccagttgcag agtgccgacg aaggcgagga
     4621 tcagcccgac gatgaaattg atcagggcga caatgcccag agcttgtgga ccgcactcct
     4681 gcatgaccag gaacgtgtcc gaccacctga actgcgcccg cccgcgcaac aacctaaaca
     4741 gagcaaccac gcattcgccc aggaatgtca gaaaaccacc cgcgccgccc gcgaacgcca
     4801 gcaccccctc gcccacccgt tccagccagg tcgcagagcg cgccccggag gcaggcgcac
     4861 gtttgtctgc agccgtccgg gccagccgca gcagattagc cgcccccggc ggcaaacccg
     4921 acgtgtccat ctcgatcccg cggtcccggc acaggtccgt gcacttcgac agaaaaacca
     4981 tcaatgcgct gttccagcgc cccagcgcag ccgtctcgaa ccccaatcgc cgcgccgagc
     5041 ccgcacggat cgaccgttcg atttcggtga aagccggcac gtccgagctc aggagccagt
     5101 cgcccgaaag ccggaccacc agactccact cccccgcggc ctccacgtcc agacgcgccg
     5161 ggctcggccc atccgcgccc aatgcagaat ggcggtggga tttcatcgtc ccgcactctc
     5221 acgctctgtt gggtttgacg acagcgatga cgtgccgatc cggggtgaga tgtcgcacgg
     5281 ccgccgcctg cacctggtct cgagtcacgg cctcgtactg cgcgtcctcc gtgtcgctgt
     5341 aggcgtagcc cagcccgaac aactcatcca gggcagccgc cagcgcgcac ccgcccacat
     5401 cctggcgcgc aatcttcttc tggccgatga tcttcgcctt ggcccggtcg agttcatcct
     5461 cggacacccc ttcggcgcgc agcagcgccg cctcgtccag gagttcggcc tcgacgcggg
     5521 ccgccgcgtc gggcgcggtg cccgcataga acgcgaagta acccggcgcc aacccgagga
     5581 agttctgggc gccgacatag taggccaaac cgagcttctc gcgaatgcgc agaaaaagcc
     5641 gcgaccccag atcgctgcag acctcctgca acacttcgag cgcaaaccga tccgcatcaa
     5701 acagcgtgcc cccgggaaag gccgccacca ccacggcctg cttcttgtcg cggatttcct
     5761 cgacgcgcac cggcccggtg cccagcgtgt cgggcgctcc cctggcccca ctcggacccg
     5821 gcccttcccc ggtccacagg ccgagcaccg cttcagcccg cgcctggacc tcctcggcat
     5881 cgatgtcgcc gtagacagcc aacaccgcat tgcggggcgc caccaagcgc cggtgaaagt
     5941 cccgcaacgg ctcgacaccg agccgcccca cgctctcctc ttcgccgagc aggtccaggc
     6001 cgtatccctc cacgccaaac atcgcgcgcc gcatcgcgcg aaaggcgctc tgaagcacgt
     6061 gatctccgcg agcccgaatt gcggcaatct ggttctggcg ctcccgttcc agggcttcca
     6121 gaggcagcgc cggacgcaac agcacatcgg ccgtcagttc gagtcccaag gccaaatcct
     6181 cccgaagcac ctccgccgtg atcccgaaac tgttgtgtcc gccgtacgtg tcgagatggc
     6241 cgccgaccga ctcgatttca ctcgccaccc ggaccgcgtc gcgcagttcg gtcccttgca
     6301 tgagcatgcg cgtgaacaac tgggtgatgc cgtttcgagc cggcgtctcc gccagaactc
     6361 cgccgcccaa aaccatgcga atctcgacaa acggcagacg tcgatcctcc ttgaccagca
     6421 gacgcagccc gttgcccagg gtgaacagcc ggaccggctc ggtctcgcgc cggacgatgg
     6481 cgaccgccgc cgcgctcctg gcgcacgccg gcaacagggc gtattgagag cggttctcgg
     6541 tcgtcagata cgcgcgagcc acccgccgca cctcgctcgg ggtcgccccc ttcaccgccc
     6601 gcagatagcg ctccgaaaaa ctcaaatctc ccgtcgcaat ccagttccca cccagatcct
     6661 gggcctggcc ttgcatcgtc ttgcgcgtcg ccagaaacgc cgcggtgaat tgcttgaccg
     6721 ccttggccag ctcgacctcg ctgacctcct ccgcgcgcac ccgctcgatc tcgctcagga
     6781 ccgcctcgcg cgccgctgcg aacttggcgc cttcgaccgt ggcgctgacg cccagcagcc
     6841 cggccttgcc cgggctgtag gtccaggcgt cgaccgacgt gaccaggccc tggcgctccc
     6901 gcaccgcctg gaacaaccgg gaactgcgcc cgctgcccag aagagtcgcc agaacgtcca
     6961 gcagcggcac atccggatgc cggacatcgg ggacatgcca gctccaatgc gcatggccca
     7021 gttcaatcgg cgcctcctcg atcacctcgc gcggcgccac ctgccgcggc tcgatcggga
     7081 aaggcgcgac gggaagaggc cgggccttgg acgccgcgaa accggcttcg acccggctga
     7141 agaccgcgtc ggcctcgacc gcgccgacca ccacgacaaa gaggttgttt ggcgcatact
     7201 tctcgcggta gtaggcgacc acatcatcgc gttgcaggcg gctgaagatg tcggggtggc
     7261 caatgaccgc gtagcggtac gggctgtgcg tgtacgccgt ctcgaacagc cgctggctcg
     7321 cgcgccggcc cggatcgtca tggcacatgt ccatctcgcg ccggatgacc tccatctcct
     7381 tctcgagttc gctcccgggc aggctggcgt tctgcatgat gtcgcacaga atatcgagcg
     7441 cgacggccga cccgctgtcg ggcgtgttaa tccag
//
LOCUS       DAOQRO010000156         7416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9657_length_7416_cov_5.971879, whole genome shotgun sequence.
ACCESSION   DAOQRO010000156 DAOQRO010000000
VERSION     DAOQRO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_7416_cov_5.971879"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..332)
                     /locus_tag="PKK20_07230"
     CDS             complement(162..332)
                     /locus_tag="PKK20_07230"
                     /inference="COORDINATES: protein motif:HMM:NF025189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HNU99716.1"
                     /translation="MIYSIMGSCRRYRIDPAKYLKDVLTRLPHLKQRDIPSLTPREWA
                     KAHPEARTLPPE"
     gene            complement(635..3568)
                     /locus_tag="PKK20_07235"
     CDS             complement(635..3568)
                     /locus_tag="PKK20_07235"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNU99717.1"
                     /translation="MSEIPKAYEPQAVESKWYQYWLEQKCFAADPARVSAKRPAYSIV
                     IPPPNVTGMLHMGHVLNNTIQDILARKARMDGKEVLWLPGTDHAGIATQAVVEKTLIK
                     QGQIRHRDDLGRDKFLEKVWEWKAKHGDIIIEQLKKLGASCDWGRERFTMDPEYSKCV
                     QRVFVDLYRKGLIYRGKRMVNWDPSARTALSDEEVEMIETKGHLWHFKYPLLDDQDRP
                     RTDAFVVVATTRPETMLGDEAVAVNPRDERYTHLVGRRILLPLQNKPIPLIADDLVDP
                     TFGTGCVKVTPAHDPADYEMAVRHKLPFTVVIGPDGRMTPEAGAAFATLTRDQARKAV
                     VAAMEQAGLLLKIEDYVHNVGHSQRSHVPIEPYLSEQWFLRYPSQAAARACVADGTMQ
                     FFPDRWAKVFDHWMGNLRDWCISRQLWWGHRVPVWTRRVPTTDTTMRMRRDRARVCAT
                     LVELARNGGAASEECPLFEDSWSGVEDHRTFWRVRGESGTDTEWEAATFSELEARALE
                     AEGWVQDPDVLDTWFSSWLWPFATMGWPERTPALEAFYPTTDLVTGPDIIFFWVARMI
                     MAGYEYMGDLPFRNVYFTGIIRDKQGRKMSKSLGNSPDPLALIARYGADALRFGTMRS
                     APLGQDVLFDEKDVELGRNFCNKLWNACRFRQMQGGAIEDEIDPALLTSDDKWILRRL
                     DRAIREIDDAFAGYRFNEATATLYRFFWSEYCDWYVEASKAALQSGRSDNPSDPPGAR
                     APDPDAAAAARRRANTLAVIDFVLSHTLRLFHPFLPFITEELWHGMNYHADLPIDQGG
                     HTIMDARWPKPLDPAFCEHYGLDETAEQVASAKYELVGVGRNFRREFNLASNRKLQFV
                     LKPAGDLPPAEVEVIRLLLNAETIEVHRAYTPPKGTPMAANALGELYLPLAGLIDSAA
                     ERARLEKELEKARLEVGKVAAKLGNPQFTQRVPPAVLEEHTKRLADWQAKEKQILTSL
                     NNLPE"
     gene            complement(3579..5534)
                     /locus_tag="PKK20_07240"
     CDS             complement(3579..5534)
                     /locus_tag="PKK20_07240"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HNU99718.1"
                     /translation="MSARAADSASPAAPAPAVPSAGSAISVRTDGPNGPWRVEGRRQR
                     LELDPANLGCRIVSGATVWQLRPSEPGDLRVVQAGKAIQLELAAAAQKTIALYDTGFQ
                     KGLKIELGGFRAESEPLDLTIQLFLCLEGPDEDLTATAIASDSAARIERCDWPSGLAS
                     ESFDATVVPFMQGMLLPKSWPEKTWLYDTLSFGRGLYQPWWGHQQGPSAMLVILETPD
                     DGGCHFEHPAGGPTHIGPRWVNSLGRWAYPRRARLCFLEQGDHVDLAKRYRRHALATG
                     HFVSLREKIARSPRVARLIGAPVVHTSILYHIQPESSYYKKDDAAHNDQLVTFEARAR
                     ELEALASRGVTNAYVHLDGWGFRGYDNLHPDVLPPCPQAGGWEGMKRFADVCDALGFL
                     FAIHDQYRDFYLDAPSYDERHTVTDAAGNRPLHSVWYGGRQSVLCSSLAPGHVRRNHT
                     AILDHGVKLRGAYLDVFSVVPPDECYQIEHPVTRTDCLRFRAECLNFVRSYGGVVSSE
                     EPSDWSVPCLDLVHHGPFALSPGPGSGPAMGIPVPLFNLVYHDALLMPWSMGRGEWGI
                     PNKDLGFLHALANGGMPYLGLDPSPDQLERVRTVTRLHARVALLEMTRHEFLDAGRRR
                     QRTTFADGTQVTIDLDRDEWKIEPALQ"
     gene            5759..5929
                     /locus_tag="PKK20_07245"
     CDS             5759..5929
                     /locus_tag="PKK20_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99719.1"
                     /translation="MRAKEAIQRTQELIRRQHKALATEGKTSFLSLGFRSVTGCGVFI
                     SFYSRPFASIRG"
     gene            6032..7348
                     /locus_tag="PKK20_07250"
     CDS             6032..7348
                     /locus_tag="PKK20_07250"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNU99720.1"
                     /translation="MTSFQLGSAAAWLRLGAAAAAIGLGLGLPTAQAGGETAESGHAR
                     PFHEAEILFPLETWHNHASCIVETPRGDLLVCWFHGSGERTADDVKIEGARKRKGSRQ
                     WSQRFTMADTPGYPDTNCAMFIDPEGRLWLLWPTILANRWESALMKYRIASDYHRDGP
                     PRWSATDILHVTPGKEFEDEIARRLPSLEQFVRESALPEQDRREAQEFLDAMRQHATD
                     KLYRRLGWMTRAHPFVLEGRRLIVPLYHDGFSFSLMAITDDWGATWRFSPPLIGGGNI
                     QPSIVRRADGSLYTLMRDNGPAPHRLMQSDSTDRGETWTPVTDSVHPNPGSGAEIIGL
                     RNGHWALVSNDTEKGRNSLSVQISDDEGKTWKWRRHLEYDTPGPEAGAYHYPSIIQAR
                     DGTLHVTYSFHLSRRALPRDVDGDPAAKTIKHAHFNEAWVSEGDSQ"
BASE COUNT         1242 a         2318 c         2514 g         1342 t
ORIGIN      
        1 ggcgtaagcg gcaacgaatg cttcgatggc ctcgcgcaac tgggcggtgc ttttgtatcc
       61 gtccggggaa ggggcagtag gcgcggcgcc ctcgatctgc tcaagtgagc ggggtgaatc
      121 gaacgagctg gtggaaaacg agcgcgccag gcgacgcgcg attactcggg cggcagcgtg
      181 cgcgcttcgg gatgggcttt ggcccactcg cggggtgtca gcgagggaat gtcgcgttgc
      241 ttgaggtggg gaagccttgt cagcacgtcc ttcaggtact tggccgggtc gatccgatag
      301 cggcggcaac tccccataat cgagtagatc accgcgctac gccgtatccg tccggggaag
      361 gggcagtagg cgcggcgccc acgatctgga gggtgtcgtt gggaaccgag tgtcgatttg
      421 cgactgcgtc ttcctgcgcg accagaccct gattcctctc tgtcccggca cggttttgcc
      481 acagcccgga tttccgatgc acgcgcccga aggtgagaaa tccgtatagg gctggtgtgc
      541 tttgctcgcg cacacgaagg ctttggagtg gggacgtcct cgtccttggc gatggaagtt
      601 gcagcaaaat cgggatcgat tcttgcacgg acacttactc cggcaggtta ttcagcgagg
      661 tgaggatttg cttttccttg gcctgccagt cggccaggcg cttggtgtgc tcctcgagga
      721 cggccggcgg aacacgctgg gtgaactgcg ggttgccgag cttggcagcg accttgccga
      781 cttcgaggcg cgccttttcg agttccttct cgagccgcgc cctctcggcg gcgctgtcaa
      841 tcaggccggc gaggggcaga tagagttcgc ccagggcgtt tgcggccatg ggcgtgcctt
      901 tgggcggagt gtaggcgcgg tggacttcga tggtctcggc gttcaggagc aggcggatga
      961 cttcaacctc ggcgggaggc aggtcgcccg cgggcttgag cacgaactgg agctttcggt
     1021 tcgacgcgag attgaactcg cgccggaagt tgcggccgac gccaaccaat tcgtactttg
     1081 cgcttgccac ctgctcggcc gtctcatcga gtccgtaatg ctcgcagaag gccggatcga
     1141 gcggtttggg ccagcgcgcg tccatgatgg tgtggccgcc ttgatcgatc ggcaggtcgg
     1201 cgtggtagtt catcccgtgc cacagctcct cggtaatgaa gggcaggaac ggatgaaaca
     1261 ggcgcagcgt gtggctgagg acgaaatcga tcaccgcaag cgtgttggcc cggcggcggg
     1321 ccgcggcggc ggcatccgga tcgggcgccc gtgcgccggg cgggtccgac ggattgtcgc
     1381 tgcgtccgga ttggagcgcg gccttgctgg cctcgacata ccagtcgcag tactcgctcc
     1441 agaagaagcg gtagagggtt gcggtggcct cgttgaaacg atagccggcg aaggcgtcgt
     1501 cgatctcgcg aatcgcccgg tcgaggcgcc gcagaatcca tttgtcatcg ctggtcagca
     1561 gggccgggtc gatttcgtcc tcgatggcgc cgccctgcat ctgtcggaac cggcaggcgt
     1621 tccagagctt gttgcagaaa ttccggccca gctcgacatc cttctcatcg aacaggacgt
     1681 cctgcccgag cggcgcgctg cgcatggtgc cgaaccgcag ggcgtcggcg ccgtacctgg
     1741 cgatgagcgc gagcgggtcg ggcgagttgc ccaggctctt ggacatcttg cggccctgtt
     1801 tgtctcggat gatgccggtg aagtagacat tgcggaacgg caggtcgccc atgtactcgt
     1861 acccggccat gatcatgcgc gcgacccaga agaagatgat gtccggtccg gtgacgagat
     1921 cggtggtcgg gtagaacgct tcgagcgcgg gcgtccgctc gggccagccc atggtggcga
     1981 agggccagag ccacgagctg aaccaggtgt cgagcacgtc ggggtcctgg acccagccct
     2041 ccgcttcgag cgcccgggct tcgagttcgg agaatgtcgc cgcttcccat tcggtgtcag
     2101 ttccgctttc gccgcggacg cgccagaagg tccgatggtc ttcgacgccc gaccaactat
     2161 cttcgaagag cgggcattcc tcggacgcgg ctccgccgtt ccgggcgagc tcgacgagag
     2221 tggcgcagac ccgggcgcga tcgcgacgca tccgcatcgt cgtgtcggtt gtcgggacgc
     2281 gtctggtcca caccgggacg cgatgtcccc accacagctg gcggctgatg caccagtcgc
     2341 gcaggttgcc catccagtgg tcgaacacct tcgcccagcg atcggggaag aattgcatcg
     2401 tgccatcggc gacacaggcc cgcgccgccg cctgactcgg gtacctgaga aaccactgct
     2461 cgctcaggta gggttcgatg gggacatggc tgcgctggct gtggccgacg ttgtggacgt
     2521 agtcctcgat cttgagaagc aagccggctt gttccatcgc ggcgacgacg gccttgcgcg
     2581 cctggtcccg cgtgagtgtg gcaaacgcgg ctcccgcctc gggcgtcatc cgtccgtcgg
     2641 ggccgatcac cacggtgaac ggcagcttgt ggcggacggc catttcgtag tcggccgggt
     2701 catgggcggg cgtcaccttc acgcagccgg tgccgaatgt cggatcgacg agatcgtccg
     2761 cgatgagggg gatcggcttg ttctgcaacg ggagcaggat gcgccgtccg acgaggtggg
     2821 tgtagcgttc gtcgcgcggg ttcaccgcca cggcctcgtc gcccagcatg gtctcgggac
     2881 gcgtggtggc cacaaccacg aaggcgtccg tgcgcggccg gtcctgatcg tcgaggagcg
     2941 gatacttgaa gtgccagagg tggcccttag tctcgatcat ctcgacctct tcgtcggaca
     3001 acgcggtgcg ggcggacggg tcccagttca ccatgcgttt gccgcggtag atcagccctt
     3061 tccggtagag atcgacgaag acccgctgga cgcacttcga gtactcggga tccatcgtga
     3121 accgctcgcg gccccaatcg cacgacgcgc ccagcttctt gagttgttcg atgatgatat
     3181 cgccgtgctt cgccttccat tcccagacct tctcgaggaa cttgtcccgg cccaggtcgt
     3241 cgcgatgccg gatttggccc tgcttgatga gcgtcttctc gaccaccgcc tgggtggcga
     3301 tgccggcgtg gtccgtgccc ggcagccaca ggacctcctt gccgtccatg cgcgccttgc
     3361 gggccaggat gtcctggatc gtattgttga ggacatgccc catgtgaagc atgccggtga
     3421 cgttgggcgg cgggatgaca atggaatagg ccgggcgttt ggcgctgacg cgcgccggat
     3481 cggccgcgaa gcacttctgc tcgagccagt actgatacca cttcgattca accgcctggg
     3541 gttcgtaggc tttcggaatc tcgctcatgt tctcaaggtc actgcagcgc gggctcgatc
     3601 ttccattcgt cgcggtccag gtcgatcgtg acctgggtcc cgtcggcaaa tgtcgtccgc
     3661 tgccgccgcc ggccggcatc gaggaattcg tgccgggtca tttcgagaag ggcgacccgc
     3721 gcgtgcagcc gggtcactgt tcggacgcgt tcaagctggt cgggactcgg gtcgaggccc
     3781 aggtacggca tgccgccgtt ggcgagggcg tggaggaacc cgaggtcctt gttcgggatt
     3841 ccccactcgc cgcgtcccat cgaccagggc atgaggagag cgtcgtgata gaccaggttg
     3901 aacagcggca ccgggatgcc catggccggc ccgcttcccg ggccggggct cagcgcgaac
     3961 gggccgtgat ggaccaggtc gaggcagggc accgaccaat cgctgggctc ttccgagctg
     4021 acgacccccc cgtaggagcg gacgaagttg aggcactcgg cccgaaaacg caggcagtcg
     4081 gtccgcgtga cggggtgctc gatctggtag cattcgtcgg gcggcacgac ggagaacaca
     4141 tcgagatagg cgcctcgcag cttcacgccg tgatcgagga tggcggtgtg gtttcggcgc
     4201 acgtggcccg gggccagact cgaacacagc accgactgcc gcccgccgta ccagacgctg
     4261 tgcagcgggc gattgccggc ggcgtcggtc accgtgtggc gctcgtcgta cgacggggcg
     4321 tccaggtaga agtcgcggta ctggtcatgg atggcaaaga ggaagcccaa cgcgtcgcac
     4381 acatcggcaa accgcttcat cccttcccat ccgcccgcct gcgggcaggg cggcaggacg
     4441 tccggatgca ggttgtcata gccgcggaat ccccagccat cgaggtgcac gtaggcgttg
     4501 gtcacgcctc gagaagccag ggcttcgagt tctcgggccc gggcctcgaa ggtgacaagc
     4561 tgatcgttgt gcgccgcgtc gtccttcttg tagtaactgg attcgggctg gatgtggtaa
     4621 agaatgctcg tgtgcaccac gggcgctccg atcagcctcg cgacccgcgg gcttcgggcg
     4681 atcttctcgc gcagcgacac gaagtgtccg gtggccagcg cgtgccgacg gtaccgcttg
     4741 gcgagatcga cgtggtcccc ctgctcgagg aaacagagcc gcgcccgacg cggataggcc
     4801 cagcgtccga ggctgttcac ccagcgcggt ccgatatgag tcggaccgcc ggccggatgc
     4861 tcgaaatggc agccgccgtc gtccggcgtt tcgaggatca ccagcatcgc cgacggccct
     4921 tgctgatggc cccaccacgg ctggtagagg ccgcgtccga agctgagcgt gtcgtacagc
     4981 caggtctttt ccggccagct cttcggcagc agcatcccct gcatgaacgg caccaccgtg
     5041 gcatcgaagg attcggacgc cagcccggac ggccaatcgc aacgctcgat ccgcgcggcg
     5101 ctgtccgagg caatggcggt ggctgtcaga tcttcgtccg gaccctcgag gcagaggaac
     5161 aactggatcg tcagatcgag cggctcggac tcggcgcgga agccgccaag ttcgatcttc
     5221 aggcctttct gaaacccggt gtcgtacagg gcgattgtct tctgtgccgc cgcggcgagt
     5281 tcgagttgga ttgctttacc ggcctggacc acgcgcaagt cccccggctc cgacgggcgc
     5341 agttgccaaa cggtcgcgcc ggagacaatc cggcagccga ggtttgccgg atcgagttcg
     5401 agccgctgtc gccgcccctc gacccgccac gggccgtttg ggccgtcggt ccggacgctg
     5461 atggcgctgc cggcggacgg gactgcgggc gcgggcgccg cgggggaggc ggagtcagcg
     5521 gcgcgggctg acacaaggag ggccaatagt gctgtcgatt gggcaaatcg aactatggcg
     5581 gcgaatgcac gcggacactg tctgtgcggt tgatattgaa acatgcgcga ttatcccgat
     5641 cggcgaagcg taacgtgacc ggacagcctg cttcaatggg tttctggctt tcgaggcagc
     5701 cggcgcaagt cctgagcacg ggtactgcag agaatccctg accgatgtac aacgcattat
     5761 gcgcgcaaag gaagccattc aacggacgca ggagttgatc cggcggcagc acaaggcact
     5821 ggccaccgag gggaaaacgt cgtttctgtc tctgggcttt cgctcggtga ccggctgcgg
     5881 cgtcttcatt agtttctatt cgcgtccgtt cgcgtccatt cgcggttaag acctcaacga
     5941 gcaaggtcca agacgatacg tttccattcg agtttggcgc gttgacgctt tgtgtggcgg
     6001 attcgcgtcc gccgtgatat ggagagcgtc catgacctca ttccagcttg ggagcgccgc
     6061 cgcctggctc agactcggcg ccgcggcggc agccatcggc ctgggtctcg ggttgccgac
     6121 tgcacaggcg ggcggggaaa cggcggagtc cggacacgcg cggccattcc acgaggccga
     6181 gattctcttc ccgctcgaga cgtggcacaa tcatgcctcg tgcatcgtcg aaacgccgcg
     6241 cggcgacctg ctggtctgct ggtttcacgg ttcgggcgag cgcaccgcgg acgacgtgaa
     6301 gatcgaaggg gcgcgcaagc gcaaggggtc ccggcaatgg agccaacggt ttaccatggc
     6361 cgacacgcct ggctatccgg acaccaattg cgcgatgttc atcgaccctg aaggccggct
     6421 ttggctgttg tggccgacaa tcctggccaa ccggtgggag agcgccctca tgaagtaccg
     6481 catcgcctcg gattaccatc gcgacggccc gccgcgctgg tccgccacgg acatcctcca
     6541 cgtcaccccc ggaaaggaat tcgaggacga gatcgcccgg cgcctgccgt ccctcgaaca
     6601 gttcgtccgg gaatccgccc tgcccgagca ggaccgccgc gaggcgcagg aattcctcga
     6661 tgccatgcgg cagcacgcca cggacaagct gtatcggcgc ctgggctgga tgacccgggc
     6721 gcatccgttc gtgctcgagg gccgccgcct catcgtgccc ctgtatcacg atggtttctc
     6781 cttctcgctc atggccatca cggacgactg gggcgcaacg tggcgtttca gtccgcccct
     6841 gatcggcggc ggcaacatcc agcccagcat cgttcggcgg gcggatggtt ccctgtacac
     6901 gctcatgcgg gacaacggcc ccgcgccgca tcggctgatg cagagtgact cgaccgatcg
     6961 gggagagacc tggacgcccg tgaccgacag tgtccatccg aaccccgggt ccggcgcgga
     7021 gatcatcggc ctgcgcaacg gccattgggc gctggtgagc aacgataccg agaaaggccg
     7081 caacagcctg agcgtgcaga tatcggacga tgaagggaag acgtggaaat ggcggcgcca
     7141 tctcgagtac gacacgcccg gccccgaagc cggcgcctac cattacccga gcatcattca
     7201 ggcgcgcgac ggcacgctgc acgtcaccta cagtttccac cttagtcgcc gggctctgcc
     7261 gcgggatgtc gacggcgatc cagcggcgaa gacgatcaag catgcccact tcaacgaggc
     7321 ctgggtaagc gagggggatt cacagtaacc cgcgtaccga gaaatcagcg agatggacta
     7381 caccgtcacc ctcacccgcc ctccgggcac cctctc
//
LOCUS       DAOQRO010000157         7308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_9904_length_7308_cov_6.415001, whole genome shotgun sequence.
ACCESSION   DAOQRO010000157 DAOQRO010000000
VERSION     DAOQRO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9904_length_7308_cov_6.415001"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKK20_07255"
     CDS             <1..268
                     /locus_tag="PKK20_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNU99721.1"
                     /translation="GKVVLEAEEIRRELKRKGELKLGQVLRLRVRYFSDGVVLGSRNY
                     VNEVFREFRDRFGARRRDGARPMRGLGALGELATMRDLKVNVVG"
     gene            complement(387..3503)
                     /locus_tag="PKK20_07260"
     CDS             complement(387..3503)
                     /locus_tag="PKK20_07260"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNU99722.1"
                     /translation="MTKTFALAPLLALGLASFAPSHGEEPSWAELERQFRELPMEARR
                     LTGPLFWLHGDESQQRLESYVAKVAEGGNGSLTTESRPHIDWLGEGWFRDVGICLEAA
                     KRHNLKLWIFDEKWWPSQGVGGQVPARYAAKKLEASAVDLTGPRPFEADGYGGESYIG
                     AIAGRAADDGAIDSESLVDLAPFINQGRLVWAVPEGRWHVMRFTFGLAPGLGQGGGRE
                     LSVDGLSTECVEWYLDTVYAPHYDRFKDDFGKTIAGFFYDEPETRGDWGTALNPVLAE
                     LGVDWKKAYVAYKFRLAGEAQTAARYQFLEARAEAWGRTMYGGITRWCQERGVESIGH
                     FMEHAGMYRLQDFCAGDLMRVQKYSSMGGIDAVFSQFKIGQRAAYDAPCWQTPKLGSS
                     ITHAYGKPDDVSMVEIFGARGQDLTYPEMKWWADHMHVSGVNFLIPHSFNPRAPRDSD
                     CPPYFYNGGFESRWPLYRVFADYTSRLTLLLTGGRHVCPVALLSPGQSLHVGQGVPVD
                     TISERLQDALYDCDWLPYEVFVNDTAVAGPELKLRAESYRVLIVPAVEAVPYAVLAKA
                     REYFEAGGVVAGYGILPSKSATIGKNEADMTALRQAIWGTPKPGLSVCRTNPRGGRSF
                     FLPLEPTPEQLQQVLGREAGVHPTLEVVEGETDHWLHVLHRVRSGRDVFFIVNQNHAG
                     PPRTFRFRITAAGEPECWDAMRNEITAVSHQRSGARTELTLTLEPNESVLLVFQQNGR
                     ALPRREPIEARLGARSIPLIRQPAPVPPEPKLDAESRFHQFFAGRFWVWYPEGNPAQA
                     APPGPCHFRKPIALPGERKLSKALFAGTADNSFVLYVNGSEAGRSDASDEGWRHPVEL
                     DVTEHMKTGLNLLAIQAVNASDRPNPAGLLGCLRVEFQSGDPLVVPVDSTWRVSREAP
                     EGWTHAGYDDADWKWADEVVLYGNAPWSTVGLGPLTLSPAKADPFVGSCEVPTDVDLD
                     RGVYLVLDGLEPEAAARVTINGKEAGGVIGNPLRLEVSMFLKYGANSVRLEPFAPRSV
                     RLVAWDKP"
     gene            complement(3632..4558)
                     /locus_tag="PKK20_07265"
     CDS             complement(3632..4558)
                     /locus_tag="PKK20_07265"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNU99723.1"
                     /translation="MNTRLAYVINPAAASGRAVARWQTARTDLWRRGLAGAEFVPRAA
                     HDGVELARRAAADADILVAVGGDGTVSEVASGLLRAGAPARLAILPLGTANDLASQLG
                     IRSLNGAIDLLPNGQTRRIDAIEVRCAQGPSNPVRHALSFVCLGFAGEVLRQTTPAIK
                     RWFGPRWCYWIGFLRALATYRHPNVRVQADQTAFHGRWLHICAGNLEWAGGGIMRLSP
                     GAQCADGLFDVCLIEAMPPPAIAWHFPKLLGGSFVKHPRARYFQGTELVLETEAPEPI
                     HIDGDMAGRTPAHLSIRPRCLHILAPPSAGHS"
     gene            complement(4555..6051)
                     /gene="rpoN"
                     /locus_tag="PKK20_07270"
     CDS             complement(4555..6051)
                     /gene="rpoN"
                     /locus_tag="PKK20_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNU99724.1"
                     /translation="MAQGLHLTQKMALQQVLAPQLQQSLALLQAPMLELKALVEHELQ
                     QNPVLEELSTSEITQQEKREIDGAPLSDPADPAEPPSDITYDPATDRVPSVVDDFQAE
                     FERLAQVDQEWRDSIVQANSPLRAREEEEERRQYMFDSLVAGTSLQEQLLEQVRLSDL
                     TDDERAVAEMIIGNIDDAGYLRASLDELAFSTSTPIDRIAQILKVVQSFHPPGVGARD
                     LRECLLLQLERAGRVASLEYAIVDKHMEALGRRRFPEIARALGKTTDEVQQASERISH
                     LEPRPGRDFMPDDHLYVVPEVFVRESADGYTVSLNNEAVPHLRISNTYKDLMANPDST
                     PEVRDYIREKIRAGKFLIKSLHQRQHTISNIAREIVGRQREFMDQGVAHLRPMTMAQV
                     AEAVGVHETTVSRAVSGKYMDTPQGVFEMKYFFTAGIPTANGEGMSNASVKGIIANMI
                     GQEDRAKPLSDEEIVKRLDASGIIIARRTVAKYRSELNILPSHLRKVY"
     gene            6178..7074
                     /locus_tag="PKK20_07275"
     CDS             6178..7074
                     /locus_tag="PKK20_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU99725.1"
                     /translation="MCVGDGSFAAPLHELALMLQWPRANALPRRPVRRIVRGVAAHFT
                     ILGSGSAGNCAYLETEETRLLIDAGLSARQIRQRLAGIERSPETLQGILITHEHMDHI
                     QGLAVLCSKLHLPVYCNRLTKEAIEHALERPVNCRLFGTGDTFEIGDVGVETFSIPHD
                     AQDPVGFLLRLDGGNIGFLTDLGHATRLVVERVRPANVLVLETNHDLKLLQEDTRRPW
                     SVKQRILSRHGHLSNEAAAGVVEQTVTDGLRHICLGHLSRDCNRPDLAIEVVAGRLQA
                     LGATHVRVDCAAQDHPSPTLTV"
BASE COUNT         1257 a         2156 c         2538 g         1357 t
ORIGIN      
        1 gggcaaggtg gtgctggaag cggaggaaat ccggcgtgag ttgaagagga agggggagtt
       61 gaagctggga caggtgttgc ggctgcgggt gaggtatttc agcgacggag tggtgctggg
      121 gtcgcggaac tacgtgaacg aggtattccg ggagttccgg gatcggtttg gggcgaggcg
      181 gcgtgacggg gcgcgaccga tgcggggctt gggagcgttg ggggagttgg cgacgatgcg
      241 ggatttgaag gtgaatgtcg tggggtgaga gtgcgtcagc aaggccggtt tagagagcgg
      301 cagggccgcg atcgatgtca ggcacgacgg cggcggggcg gacagtcact ggcctctgcc
      361 atgcaaccga gccgggatcg ccatcgttaa ggtttatccc acgcgactaa gcggacgctt
      421 cgaggggcga aaggttcgag tcgaaccgaa ttggcaccat acttgaggaa catcgagact
      481 tcgagcctga gggggttgcc tatgacaccg ccagcttctt tgccgttgat cgtcacgcgc
      541 gcggccgctt cgggctcgag accgtccaag acaagataca cgccgcgatc gaggtcaacg
      601 tccgtgggta cctcacacga tcctacgaac gggtccgcct tagccgggct cagagtgagc
      661 gggccgaggc cgacagtgga ccagggggcg ttgccgtaca gcaccacttc gtccgcccat
      721 ttccagtctg catcgtcgta accggcatgc gtccagccct cgggcgcttc gcgggacacc
      781 cgccacgtgc tgtccacagg cactacaagc ggatcgccgc tctggaactc gacacgcaaa
      841 cagcccagca gtccggccgg gttggggcga tcgcttgcgt tgaccgcctg gatggcgagc
      901 aggttcaggc cggtcttcat gtgctcggtg acgtccaact cgaccggatg ccgccatcct
      961 tcgtcgctgg catcactgcg tcccgcttcg ctcccattga catagagaac gaagctgttg
     1021 tccgccgtgc cggcgaagag ggcctttgaa agcttgcgct ccccgggcaa cgcgattggt
     1081 tttcggaagt ggcaggggcc cggaggtgca gcctgggccg ggttgccttc cgggtaccag
     1141 acccagaacc gtcccgcgaa gaactggtgg aagcgggact cggcatcgag tttgggctcg
     1201 ggcgggacgg gggctggttg tcgaatcaga gggatgcttc tggcgccgag ccgagcctcg
     1261 attggttcgc ggcgcggcag cgcccgaccg ttctgttgaa acacaagcag gacgctttcg
     1321 ttcggttcga gcgtcagcgt cagctccgtc cgcgcgcccg accgctggtg ggatacagcg
     1381 gtgatttcgt ttcgcatggc gtcccagcac tccggctccc ctgcggcggt gattcggaat
     1441 ctgaacgtgc gcggcggacc ggcatggttt tggttgacga tgaagaacac gtcgcgtccg
     1501 gatcgcaccc ggtgcaggac atgcagccaa tggtcggttt caccttcgac aacctcgagg
     1561 gtcgggtgca ctcccgcctc gcgccccagg acctgctgaa gctgttcggg ggtcggttcg
     1621 aggggcagga aaaaggagcg tccgccccgg ggatttgtgc ggcaaacgct caagcccggt
     1681 ttgggggttc cccaaatcgc ctggcgcaac gcggtcatgt cggcttcgtt cttgccgatt
     1741 gtggcagact tcgaggggag gatgccgtag ccggccacga ctcctccggc ctcgaaatac
     1801 tctctggcct ttgccagcac ggcataaggc accgcctcga ctgccggcac aatcaacacc
     1861 cgataggact cggcgcgcag cttgagctcc gggccggcca cagcggtgtc attcacaaag
     1921 acctcgtagg ggagccagtc acaatcgtag agggcgtcct gcaagcgctc gctgatcgtg
     1981 tcgaccggga cgccctggcc gacgtgcagg ctttggccgg gcgaaagcag cgccacggga
     2041 cagacatgtc gtccgccagt gagcaggaga gtcagccggc tggtgtaatc ggcgaagacg
     2101 cggtagagcg gccagcgtga ttcgaagccg ccgttgtaga aatagggcgg acaatccgag
     2161 tcacgcgggg ctcgcggatt gaaggagtgg gggatgagga agttgacgcc cgacacgtgc
     2221 atgtgatcgg cccaccactt catctccgga taggtcaggt cctgaccgcg cgccccgaag
     2281 atctcgacca tgctgacatc atcgggtttg ccgtaggcat gggtgatcga gctgccgagc
     2341 ttgggcgtct gccagcacgg tgcgtcatac gcggcacgct ggccgatctt gaactgggaa
     2401 aacacggcgt cgatgccgcc catgctgctg tatttctgga cacgcatgag gtcgcctgcg
     2461 cagaaatcct gaagccgata catgccggcg tgttccatga aatggccgat ggactcgacg
     2521 ccccgctcct ggcaccagcg tgtgatgccg ccgtacatcg tgcgacccca ggcctccgcg
     2581 cgggcttcga ggaactgata gcgagcggcc gtttgggcct cgcccgccag tctgaatttg
     2641 taggccacgt aggccttctt ccaatcgacg cccaactcgg ccaggacggg attcaaggct
     2701 gtgccccagt cgccgcgggt ttccggttcg tcgtagaaga acccggcgat cgtcttgccg
     2761 aagtcatctt tgaaccggtc gtaatggggc gcgtagacgg tgtcgagata ccactcgaca
     2821 cactcggtgc tcaggccgtc cacgctcagt tcacggccgc cgccctggcc caagccgggc
     2881 gcgagtccga aggtgaacct catgacatgc catcggcctt cggggacggc ccagaccagg
     2941 cggccctggt tgatgaacgg cgccaggtcg accaggctct cgctgtcgat cgcgccatcg
     3001 tcggctgcgc gtccggcgat tgcgccgatg tagctctcgc cgccgtagcc atcggcctcg
     3061 aacgggcgcg gtccggtcaa gtcgactgcc gacgcctcga gcttcttggc ggcataccgg
     3121 gcgggaacct gtccgccgac gccctgactg ggccaccatt tctcgtcgaa gatccagagc
     3181 ttgaggttgt ggcgttttgc ggcttctaga cagatgccga cgtcgcgaaa ccatccttct
     3241 ccgagccagt cgatgtgtgg gcgcgactcg gtggtgagag agccgttgcc gccctcggcg
     3301 accttggcga cgtaggactc gagccgctgc tgcgattcgt cgccgtgcag ccagaacaac
     3361 ggtccggtca gccgccgcgc ctccattggc agctcgcgga attgccgttc gagctcagcc
     3421 cacgaaggtt cctccccgtg ggatggcgcg aacgacgcga gtcccaatgc caggagaggg
     3481 gccaaggcga aggtctttgt catagtcaca gatgcgtttc gcgacagtga atcgcatgga
     3541 gccgacgaat gcaaggcaca gcctcgcgca gacacgcaca cgacgggcgc acgcacctcg
     3601 gcccgaacgg ctgaccgcgt ctggcatgga atcaggaatg gcctgcgcta ggcggcgcga
     3661 ggatgtggag gcatcggggc cggatgctca ggtgagccgg ggtgcggcct gccatgtcgc
     3721 cgtcgatgtg gattggctcg ggcgcctcag tttccagcac gagttccgtg ccttgaaagt
     3781 agcgggcgcg ggggtgtttg acgaacgaac cgcccagcag ctttggaaaa tgccaggcga
     3841 tggccggggg cggcattgcc tcgatcaggc agacatcgaa caggccgtcg gcgcactggg
     3901 ctccggggga caagcgcatg atgccgccgc cggcccactc gaggtttccg gcgcagatgt
     3961 ggagccaacg gccgtggaat gcggtttgat cggcctgcac tcggacgttg gggtggcgat
     4021 aggtggcgag ggcgcgcagg aacccgatcc aatagcacca gcggggtccg aaccagcgct
     4081 tgatggcggg cgtcgtttgc ctgaggactt cgccggcgaa gccgaggcag acaaaggaga
     4141 gggcgtggcg gactgggttg gacgggccct gcgcgcacct cacttcgatg gcgtcgattc
     4201 ttcgggtttg gccgttggga aggagatcga tcgccccgtt caaggagcga attcccagtt
     4261 ggctggccaa gtcgttggct gtgcccagcg gcaggatggc caggcgggca ggggccccgg
     4321 ctcgcaagag gccgctggcc acctcggaca ccgtcccgtc gccgccgacg gcgaccagga
     4381 tgtcagcgtc cgcagccgct cggcgcgcca attcgactcc gtcatgggct gcccgtggga
     4441 cgaactccgc gccggcgaga ccgcgccgcc acagatcggt ccgggcggtt tgccatcgtg
     4501 ccacggctcg gcccgaggcg gcggcgggat tgatgacata ggcgaggcgc gtgttcaata
     4561 gaccttcctc aggtgggatg gaaggatatt gagttcggac cgatacttgg cgactgtgcg
     4621 gcgggcgatg atgatgccgg aggcgtcgag gcgtttgacg atttcctcgt cagacagcgg
     4681 tttggcgcgg tcttcctgtc cgatcatgtt ggcgatgatg cctttgacgc tggcgttgga
     4741 catgccctcg ccgttggctg tcgggatgcc tgccgtgaag aagtacttca tctcgaaaac
     4801 gccctggggt gtatccatgt acttgccaga gacggcgcgg ctgacggtgg tttcgtgcac
     4861 gccgacggct tcggccacct gggccatggt cattgggcgc aggtgcgcga ctccctggtc
     4921 catgaactcg cgctgccgtc cgacgatttc gcgggcgata ttcgagatgg tgtgctgtct
     4981 ctggtgcagg ctcttgatca ggaatttccc ggcgcggatc ttttcgcgga tgtagtcgcg
     5041 gacctccggc gtgctgtcgg ggttggccat gaggtccttg taggtgttgc tgatgcgcag
     5101 gtgggggacg gcttcgttgt tgagcgagac ggtgtagccg tcggccgatt ctcgcacgaa
     5161 tacctcgggc acgacgtaga gatgatcgtc gggcatgaaa tcgcggcccg gacgcggttc
     5221 gaggtggctg atgcgttcgc tggcctgctg gacttcgtcg gtcgtcttgc ccagggcgcg
     5281 tgcgatttcg gggaagcggc ggcggcccag agcttccatg tgcttgtcga cgatggcgta
     5341 ttcgagcgag gcgacgcggc ctgcgcgttc gagttgcagc aggaggcact cccgcaagtc
     5401 ccgggcgccg acgccaggcg gatggaacga ctggaccacc ttgaggatct gggcgattcg
     5461 atcgatgggc gtactggtgg agaaagcgag ttcatcgagg ctggcccgca ggtagccggc
     5521 gtcgtcgatg ttgccgatga tcatctcggc caccgcgcgt tcgtcgtcgg tcaaatccga
     5581 gaggcgcact tgttcgagca actgctcctg gagcgaggtg ccggcgacca gcgagtcgaa
     5641 catgtactgg cgccgttcct cctcttcttc gcgggcgcgc aagggcgagt tggcctggac
     5701 gatggaatcg cgccactcct gatcgacctg ggcaaggcgc tcgaactcag cctgaaagtc
     5761 gtccacgaca ctcggaaccc ggtcggtcgc cggatcgtag gtgatgtcgg acgggggttc
     5821 ggcgggatcg gcgggatcgg aaaggggggc gccatcgatc tcgcgttttt cctgctgggt
     5881 gatttcgctg gtggagagtt cttcgagaac cgggttctgc tgcaattcgt gctcgacgag
     5941 cgccttgagt tcgagcatcg gggcctggag gagcgccagc gattgctgga gctgtggcgc
     6001 caacacctgt tggagcgcca tcttctgggt cagatgcaag ccctgcgcca tgctgctcat
     6061 cttagggggc agccccaaat tcacaagcgc gatctcaatg ccgcaacagc gccaattcat
     6121 gaagtcgaaa aggtcggagt ggggacggct cgttccccgc catgtcaagt gaagacggtg
     6181 tgtgtcggcg acgggagctt cgccgctcca cttcatgaat tggccctgat gctgcaatgg
     6241 ccgcgggcaa atgctttgcc acgccggccc gtccgccgta ttgtccgggg cgtggcggcg
     6301 catttcacga ttttgggcag cgggtcggcc ggcaactgcg cctacctcga aaccgaggag
     6361 acgcgcctgc tgatcgacgc gggcttgagc gcccggcaaa tccgccagcg actggccggg
     6421 attgaacgtt cgccggagac gctgcaaggg attctgatca cgcacgagca catggatcac
     6481 atccaggggc tggcggtgtt gtgctcgaaa ctgcacctgc cggtctattg caaccggctg
     6541 acgaaggagg cgatcgagca cgccttggag cggcctgtga attgccgtct atttggcaca
     6601 ggcgacactt tcgagatcgg cgacgtaggc gtcgagacct tctcgatccc tcatgacgcg
     6661 caggacccgg tgggttttct gctgcgtcta gatggcggaa acatcggttt cctcaccgat
     6721 ctgggacacg ccacccggct ggtcgtcgag cgggtgcgtc cggccaacgt gctggtcctc
     6781 gagacaaacc acgatctgaa gctgctgcag gaggacacgc gccgcccctg gagtgtcaaa
     6841 caacggattc tgagccggca cggccatctc tcgaatgagg ctgccgcggg tgtggtcgag
     6901 caaacagtta cggacggtct gcgccacatc tgtctggggc atttgagccg ggattgcaac
     6961 cgtccggacc tggcgatcga ggttgtggcc gggcggcttc aggctcttgg ggccacgcat
     7021 gtgcgggtgg actgcgcggc gcaggaccat ccgagcccaa ccttgacggt gtgagcaccg
     7081 cggtgaggcg atggacaatc caagcgggca cacgagcggg cggggcggtc tgcggcgccg
     7141 ggttcccgcg ctctttcgag ccacgatgcg atcgcgatgc gggctagcag cctgtcggac
     7201 ttttccccaa caattttgcc aggcgtttga acaagagggc gaggttcaga gttcagcaga
     7261 taactatggg cccacgcttc gtgaacgtcg accgtgacac ccccctgc
//
LOCUS       DAOQRO010000158         7239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10051_length_7239_cov_4.523385, whole genome shotgun sequence.
ACCESSION   DAOQRO010000158 DAOQRO010000000
VERSION     DAOQRO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10051_length_7239_cov_4.523385"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKK20_07280"
     CDS             <1..403
                     /locus_tag="PKK20_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99726.1"
                     /translation="AHRRIREFMEVRGIGIINVADMFGVKSIRTHKRVDLVVTLKPWD
                     EVANVERVGLDQEKIPLLGIEVPHVTIPVAPGRDLARLVEVAAFLVKLRLAGHNPAKD
                     LETRLLADMAGSRREPHGLPEVADHETRLES"
     gene            438..2504
                     /locus_tag="PKK20_07285"
     CDS             438..2504
                     /locus_tag="PKK20_07285"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNU99727.1"
                     /translation="MNPFAAFAAAASGVGHRHVLTPRRRRSRIGAGGLLAAAACLVSA
                     TMLAAGSQRITTASLIEDMTDLAGLAEFPNPAYTCKQFSSYDRKSKSPTEDWFANADA
                     GHFLRKEQRAGREEFVMVDTAGPGALVRIWSANPAGTVRVYLDGAEEPVIEAAMPDLL
                     GGKFPGLPRPIAGEYSKGWNLYLPIPYARSCKVTSDKGGFYYHVNYRTYPQGTSVKTF
                     DRGDLQTLASRLERLAAQLEKPRSVTAADGQVTPFEIVVGAGDQLRHTFYGPAAITRI
                     VLRAQAADVEAALRGLVVKVTFDGQETIASPLGDFFGSAPGINPYASLPLGMTEDGEM
                     YCHWVAPFQDSAVIELTNHSGQDVTLSGEITVSDYRWTESSMHLHAKWRVEFDVPTRP
                     MQDWNYLTVQGRGVFVGVAFAIDNPVKDWWGEGDEKIYVDGEVFPSHFGTGTEDYFGY
                     AWCWPGLFTHAYHAQSRCDGPNNYGRTSVNRFHILDRIPFMRDFKFDMELWHWNETCK
                     VNMAVVAYWYARPGATDRFKAIRAEDTELRPMPVYVVPRVAGAIEGEGMRIVRVTGTV
                     EPQGWDGVSGGSHLWWHAGMKPGDVLTVAFPAPKQGQYRVMGHFLRARDYGVHQLAIN
                     GQKTGDPIDFYNPTVQPSKEIDLGVYTLKEGENEFSVTIVGANDKAEKAYMFGLDYLL
                     LKPVSP"
     gene            2501..3541
                     /locus_tag="PKK20_07290"
     CDS             2501..3541
                     /locus_tag="PKK20_07290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNU99728.1"
                     /translation="MKRRDFLRMVGGVTAAGGLGLERLLADFKADDDIVGRVAGLPRR
                     VLGRTQQQISIVGFPGLGLIRVDQEGANKAVREAFDRGVNYYDVAPAYGNDGECEIKL
                     GPALRQLDRSKVFLGCKTKARDKEGARTELERSLKRLQTDHFDLYQLHHLVTVDEVKR
                     AFGPGGAFETFLAARDEGKVKWFGFSAHSTKAALAALEAYAFDTVMFPISFADYYLRD
                     FGRAVIDAAAKRGSAVIAIKPLSMGAWPKDVPRTRSWWYRTTETPEEVSLALRFSLSL
                     RGVITGIPPSYLDLLDMSIAAAKSYKPATVAEKDELRVLAEKCEALFLREDQRGDTAG
                     VPSACPYPDHLV"
     gene            3732..4250
                     /gene="coaD"
                     /locus_tag="PKK20_07295"
     CDS             3732..4250
                     /gene="coaD"
                     /locus_tag="PKK20_07295"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNU99729.1"
                     /translation="MRTRRPLRLGVYAGSFDPATVGHLWMINQGVRLFDRLVVAVGLH
                     PEKRCTFSLDARLEMLRESVRKFRNVSVVTFSERYLIHFAQSVGATHLLRGIRSESDY
                     EYERTMRHINGDLDAKICTVFLMPPRDVAEVSSSMVKGLVGPQGWQRVVRQYVPPPVY
                     KALLRFRRSTSV"
     gene            4262..6355
                     /locus_tag="PKK20_07300"
     CDS             4262..6355
                     /locus_tag="PKK20_07300"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNU99730.1"
                     /translation="MQDTSTLLVCALLVLPALAGESGATPITLGHLVDEMADLERLAR
                     WPAPAYRTVQFSSYDRRSTTAEAPQWFSNADGFGREPIPGFLKVLRPPQDGQAGLYLV
                     AEASGPGAIVRGWSAGMGGVLRVYLDPPDDTAEAGKEMPVWEGDAYEFLARRSAHYMK
                     SAGVDLEARDAFIQQDADYFPIPFQNGMKVTWEGKVSDLHFYHLQIRQYPVGTAVRTF
                     DPQRDVREAEARIRAAVEALTRPRVVLGDEPVALEGAIEPGRNWTWSPPGQGPSAVRE
                     FKLRVESDPLDAALRGCLLRISFDGSQRPQVEAPVGDFFASGPGVNPFSSLPFSVDAD
                     GALTSRFVMPFEKSVRIEIVNHTSRPVRLTGSVRLSRWSWDERSLYFRAKWRADHDLL
                     AGAGVIDLPCLVALGRGVFAGCAVMIMNPSGVPTAGGNWWGEGDEKILIDGELMPSMF
                     GTGTEDYFNYSWSRPDLFDHPYCGQPLDSGPDTAGYISNHRFQVLDAVPFERSAAVFL
                     ELWSHNRTPGLSYARVVYHYARPNAIDDHRGLMPADLRIPLLPAREPQAAGGATGARF
                     HYFEKLGLEATAGRIQTVPFPLATQLRLVLWQAEKGARLKFNLPVEKDGRYAIHFVAL
                     HRPDAATVRVLVDDRPLATGSGAEVVSLKSAHAPRMLNVGFKAVHLEAGSRAVAIECI
                     EPGVVGLDYLWERNP"
     gene            complement(6421..>7239)
                     /locus_tag="PKK20_07305"
     CDS             complement(6421..>7239)
                     /locus_tag="PKK20_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99731.1"
                     /translation="TLGFCAFLANLGHGGGGGGGGHWGRHGGDIHVQPMIEWIESNLA
                     LVVLGALGIGVIVLALMLLFAWLSSRGEFMFVDGVVRNRAAVKEPWAVYAQSGNRLFL
                     FTFLLWLALSLFVAITLGSSLAWAWPDIQADRFGDRSLVAILCGIGVLLPTLLGSWIA
                     HVVLRDFVVPAMYLRPQPVLAGFSSVWQEIMRPHLGIVLLYFLVKILLGLVIGLLAVI
                     VTCLTCCLAALPYIGTVILLPLLVFKRSFSLCFLQQFGPAWDCFASAPSPAQTH"
BASE COUNT         1296 a         2093 c         2472 g         1378 t
ORIGIN      
        1 ggcgcaccgg cggattcgcg agttcatgga agtgcgaggg atcggcatca tcaacgtggc
       61 cgacatgttc ggcgtaaaga gcattcggac ccacaagcgg gtcgatttgg tggtgacctt
      121 gaagccatgg gacgaggttg ccaatgtcga gcgggtgggc ctcgatcagg agaagattcc
      181 cctgctgggc atcgaggtcc cgcatgtgac cattcccgtg gccccgggcc gggatctggc
      241 aaggctggtc gaggtggcgg cattcctcgt caagcttcga ctcgcgggcc acaatcccgc
      301 caaggatctc gagactcgat tgttggccga catggcgggc tcccggcgcg agccgcatgg
      361 gctccccgag gttgcggatc atgaaacgcg tctcgagtct tgattctctc gctcgaatcc
      421 tcagacttgc gcccactatg aatccattcg ctgcattcgc cgccgccgcg tccggcgtgg
      481 ggcaccgaca cgttctcaca ccgcgccgcc gccggagccg gattggggcg ggtgggctat
      541 tggccgccgc ggcctgcctg gtgtcggcga ccatgcttgc ggcaggttcg cagcggatca
      601 cgacggcgtc gctgatcgag gacatgaccg acctggcggg tctggcggag ttcccgaatc
      661 cggcctacac ctgcaaacag ttctcgtcgt atgaccggaa atcgaagtcg ccgaccgagg
      721 attggtttgc gaatgccgac gcggggcatt ttctgaggaa ggagcaacgg gccggacggg
      781 aggagttcgt catggtcgac acggcgggtc cgggcgccct ggtgcgaatc tggtcggcca
      841 acccggcggg cacggtgcgg gtttacctgg acggcgcgga ggagccggtg attgaagcgg
      901 cgatgcccga tcttctgggc ggcaagttcc ccgggttgcc acgccccatt gcgggcgagt
      961 acagcaaggg gtggaatctc tacctgccga tcccgtacgc gcgtagctgc aaggtgacca
     1021 gcgacaaggg cggtttctat tatcatgtga actaccgcac ctatccacag gggacgagcg
     1081 tcaagacgtt cgatcgcggg gatctgcaga ctctggcgtc gcgcctcgaa cggctggccg
     1141 cccagctcga gaagccgcgc tcggtcaccg cggcggatgg gcaggtgacg cctttcgaga
     1201 tcgttgtggg cgcgggcgat caactgcgcc acacgttcta tggccctgcg gcgatcaccc
     1261 ggattgtttt gcgcgcgcag gccgccgatg tcgaagccgc attgaggggt ttggttgtga
     1321 aagtcacgtt cgatggccag gagacgatcg caagcccgct gggcgacttt tttggcagcg
     1381 cgcccggcat caatccatat gcttcgctgc cgctgggcat gaccgaggac ggcgagatgt
     1441 actgccactg ggtcgcgccg ttccaggatt cggcggtgat cgagttgacg aaccacagcg
     1501 ggcaggacgt gaccctttcg ggcgagataa ccgtgagcga ctatcgctgg accgagtcgt
     1561 cgatgcactt gcacgccaag tggcgagtcg agttcgatgt gccgacgcgt ccgatgcagg
     1621 actggaacta tttgacggtg caagggcgcg gggtgtttgt cggggtcgcg ttcgccatcg
     1681 acaatccggt gaaggactgg tggggtgaag gcgacgagaa aatctacgtc gatggcgagg
     1741 tttttccgag ccacttcggg accgggacgg aggactactt tggctatgcg tggtgttggc
     1801 ccggcctttt cacgcacgct taccatgccc agtcacggtg cgacggtccc aacaactacg
     1861 ggcggaccag cgtgaaccgc tttcacatcc tggaccggat tccgttcatg cgcgacttca
     1921 agttcgacat ggaactctgg cactggaacg agacctgtaa ggtcaacatg gcggttgtgg
     1981 cctactggta cgcccgtccc ggagcgaccg accgcttcaa agccatccgt gccgaggaca
     2041 ccgaactgcg tcccatgccc gtctatgtcg tgccgcgcgt cgcgggtgcg attgagggtg
     2101 aggggatgcg gattgtgcgc gtgacgggca cggtcgagcc gcagggttgg gacggtgtca
     2161 gcggggggag tcacctttgg tggcatgcgg gcatgaagcc cggggatgtt ctcaccgttg
     2221 ctttcccggc accaaagcaa ggccagtacc gcgtcatggg ccatttcctg cgtgcccggg
     2281 actacggcgt gcatcaactg gcgatcaacg ggcagaagac gggcgatccg atcgatttct
     2341 acaatcccac cgtccaacct agcaaggaga tcgatctggg cgtttacacg ttgaaggagg
     2401 gggagaacga gttctccgtc acgattgtcg gcgccaacga caaggcagag aaggcgtaca
     2461 tgttcggact cgattatctc ctgctcaaac cggtatcccc atgaaacgac gagacttcct
     2521 gcgcatggtc ggcggcgtga cggccgccgg tggtctgggc ctcgaacggc tgttggcgga
     2581 tttcaaggcc gacgacgaca ttgtggggcg cgttgcgggt ctgccgcggc gcgtcctcgg
     2641 ccgaacgcag cagcaaattt cgatagttgg ctttccaggc ctgggactga tccgggttga
     2701 ccaggagggc gcgaacaagg cggtgcgcga ggccttcgat cggggtgtga actactacga
     2761 cgtggcgccc gcttacggga acgacggcga gtgcgaaatc aagctcggcc ccgccttgcg
     2821 gcaactcgat cgcagcaagg tttttctggg gtgcaagacc aaggcccgcg acaaggaagg
     2881 ggctcggacc gaactcgaac gctcgctgaa gagacttcag accgatcact tcgatttgta
     2941 ccagcttcat cacctggtga cggtcgatga agtcaagcga gcgttcgggc ccggcggggc
     3001 gttcgagacg ttcctggcgg cgcgcgacga ggggaaggtg aagtggtttg gtttttcggc
     3061 ccacagcacc aaggcggcgc tcgccgcgct cgaagcctat gcgttcgaca ccgtgatgtt
     3121 tcccatcagt ttcgccgact actacctgcg ggatttcggg cgtgcggtga tcgatgcggc
     3181 ggcgaagcgc ggttcggcgg tcattgcgat caaaccgctg tcgatgggcg cgtggcccaa
     3241 ggacgtgccg cggacgcggt cctggtggta tcgcacgacc gagacgcccg aggaggtgtc
     3301 gctggccctt cgattttccc tctcgctgcg aggggtgatc actggcattc cgccttccta
     3361 cctcgatttg ctggacatgt cgattgccgc ggcgaagagc tataagccgg cgacagtcgc
     3421 cgagaaggac gaactgcggg tcctggccga gaagtgcgag gcgctgttcc tccgggagga
     3481 tcagcgcggc gacacggccg gggtgccgtc tgcgtgtccg tatccggacc atctggtgta
     3541 agcggcgggg gacacggtcg cggtcttgcc ctcgatggcc cgcgtgttat cgggggcgac
     3601 ggagccgccg ccaacaagcc gcggctggaa ccggatgttg ccaacacgtc gttcgtgtgt
     3661 gcgcgggctg ggatcgctgc atcatgcggt tggtggctga caagatcggg agtcggtgta
     3721 gagtcgggcg gatgcgaacc agaagacccc ttcggcttgg cgtttatgca ggcagcttcg
     3781 atccggccac ggtggggcat ttgtggatga tcaaccaagg cgtgcgtttg ttcgaccggt
     3841 tggtggtggc ggtgggcctg catccggaaa agcggtgcac gttttcgctc gatgcgcggc
     3901 tcgaaatgct ccgggaatcg gtgcggaaat ttcggaatgt gtcggttgtg accttctccg
     3961 agcgttatct gattcacttt gcgcagtcgg ttggagcgac acatttgttg cgcggcatcc
     4021 ggtccgagag cgactacgag tatgagcgga cgatgcggca catcaacggc gatctggacg
     4081 ccaaaatctg caccgttttc ctgatgccgc cgcgcgatgt cgccgaagtc agttcgagca
     4141 tggtcaaagg cctggtcggt ccacaaggct ggcagagagt cgttcgtcaa tacgtgccgc
     4201 cgccggtgta caaggccctt ttgcggtttc gtcgctcgac ctccgtttga aaggaattcc
     4261 catgcaagac acctcgactc ttcttgtctg cgccctgctc gtgctgccgg ccttggccgg
     4321 cgaatccggc gcgacgccca ttacactggg ccacctggtc gacgagatgg cggacttgga
     4381 acgtctggcc cgatggcctg cgcccgccta tcgcacagtg cagttttcga gttatgaccg
     4441 gcgctcgaca accgcggagg cgccccaatg gttctcgaac gcggacggtt tcgggcgcga
     4501 gccgattcct ggttttctga aagtgctgcg tccgcctcag gatggccagg ccggcctcta
     4561 cctggtggcg gaggcgtccg gcccgggtgc gatcgttcgc ggctggtcgg ccggcatggg
     4621 aggcgtgttg cgggtttatc tggacccgcc ggacgacaca gcggaggctg ggaaggagat
     4681 gccggtttgg gaaggggacg cctacgagtt tctcgcgcgg cgttcggctc actacatgaa
     4741 gtcggccggg gtcgatctcg aggcgaggga cgcgttcatt cagcaggatg cggactactt
     4801 tccgattcca ttccagaacg ggatgaaggt gacgtgggaa gggaaggtgt ctgatctgca
     4861 cttttatcat ctccagattc ggcagtaccc ggttggcacc gcggtgcgga ccttcgatcc
     4921 gcagcgcgac gtgcgagagg cggaagcgcg aattcgcgcc gcggtcgagg ctctaacccg
     4981 gcctcgagtg gtgttggggg acgaaccagt ggcactcgaa ggcgcgatcg agccgggacg
     5041 caactggaca tggtcgccgc ccggtcaggg gccgtcggcc gtgcgcgagt tcaagctgcg
     5101 tgtcgaatcc gatccgctgg acgcagcgct gcggggctgc cttttgagga tttcgttcga
     5161 cggatcccaa cgtccgcaag tcgaggcgcc ggtgggcgat ttcttcgctt ccggcccggg
     5221 tgtgaatccg ttttcgagcc tgccgttcag tgtggatgcg gacggcgcgc tgaccagccg
     5281 gtttgtgatg ccgttcgaga agtcggttcg gatcgagatc gtcaaccaca ccagccgccc
     5341 ggtgcgtctc accggttcgg tccgcctttc ccgatggagc tgggacgaac gctcgctcta
     5401 tttccgcgcg aaatggcggg ccgatcacga tctgctcgcc ggggcgggag tgatcgattt
     5461 gccgtgcctg gttgctcttg gccgaggggt ctttgcgggc tgcgcggtga tgatcatgaa
     5521 tccgtcgggc gttccgaccg ccggagggaa ctggtggggt gaaggcgacg agaagattct
     5581 gatcgacggc gagttgatgc catcgatgtt cgggaccggc accgaggact acttcaacta
     5641 ctcgtggagc cgaccggacc tgttcgatca cccgtactgc ggtcagccct tagattcggg
     5701 tcctgacacc gcgggttata tctcgaacca caggtttcag gtgctcgatg cggtcccatt
     5761 cgaacgatcc gcggccgtgt tcctcgaact ctggtcgcac aatcgcacgc ccggcctgag
     5821 ctacgcccgc gttgtctacc attacgcgcg tccgaacgca atcgacgatc atcgcggact
     5881 catgcctgcc gacctgagaa tacctttatt gccggcacgc gagccgcagg cggcgggcgg
     5941 cgctactggc gcgcgctttc actacttcga gaagctcggt ctcgaagcca cggcgggccg
     6001 gatccagacc gtgccgttcc cgttggcgac gcaattacgg ttggttttgt ggcaggcgga
     6061 gaagggtgct cggctgaagt tcaatcttcc tgtcgagaag gacgggcgct acgccatcca
     6121 ttttgtggcg ctgcatcgtc cggatgcggc gacggtgcga gtgttggttg acgacaggcc
     6181 tcttgcgacc ggaagtgggg cggaggtcgt ctcgctgaag tcggctcacg ccccccgtat
     6241 gctcaacgtc ggattcaagg cggtgcatct cgaagccgga tcaagggctg tggccatcga
     6301 gtgcatcgag cccggtgttg tgggtttgga ctatctgtgg gagcggaatc cctagccgga
     6361 tacttcatca ggcttcgcgg agtttgatgt ccgggctctg gctctcttcg cgggtcgcga
     6421 tcagtgtgtc tgggctggtg aaggcgcgga ggcgaagcaa tcccaagccg ggccgaactg
     6481 ctggaggaaa cagagcgaga agctgcgttt gaagaccagc agagggagga ggatgacggt
     6541 gccgatgtag ggaagggctg cgaggcagca ggtcaggcac gtgacgatga cggccagcag
     6601 gccgatgacc aagcccagaa gaatcttcac caggaagtag aggagcacga tgcccaggtg
     6661 ggggcgcatg atctcctgcc agaccgaact gaagcccgcc aagaccggtt gcggccgcag
     6721 gtacattgcg ggaaccacga aatcgcgcag aacgacatgt gcgatccagg aaccgagcaa
     6781 tgttgggagc agcacgccga tgccgcagag gatggcgacg agggagcgat caccgaaacg
     6841 gtcggcctgg atgtcgggcc aggcccaagc caggctggac cccaacgtga tcgccacaaa
     6901 cagggacagc gccagccaga gcaggaacgt gaacagaaac aggcggtttc cggattgggc
     6961 gtagacagcc cacggttcct taacggcggc gcgattgcgc acgacaccgt ccacgaacat
     7021 gaactcgccc cggctgctca gccatgcgaa cagcagcatc agggctagga cgatcacgcc
     7081 gatgcccagg gcccccaata ccaccaaggc cagattcgat tcgatccatt cgatcatggg
     7141 ttggacatga atatcgcccc catgcctgcc ccaatgaccg ccgccgcccc cgccgccccc
     7201 atgcccgagg ttggcgagga aggcgcagaa gcccagtgt
//
LOCUS       DAOQRO010000159         7219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10094_length_7219_cov_4.396985, whole genome shotgun sequence.
ACCESSION   DAOQRO010000159 DAOQRO010000000
VERSION     DAOQRO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10094_length_7219_cov_4.396985"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="PKK20_07310"
     CDS             <1..1894
                     /locus_tag="PKK20_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HNU99732.1"
                     /translation="LRRARLPGPRHARLDDAGAEGGAARIVDHAGHRLLDDREMRLRD
                     AERRHQLTGFFFWAAWACAANRPGETVSYTQNWPPEPLVANTPTGSSVLWSVVSFVLL
                     LAGIGALAWYNAALKGREEQPHDLPEKDPLLGLQPTPSMRATLKYFWVVVALIMAQII
                     MGVVTAHYGVEGNGFYGIPLAEWLPYSVTRTWHTQLAVFWIATAWLATGLFIAPAVSG
                     HEPKYQRAGVNFLFVCLLIIVVGSMFGTWYGTRQRMGYEMNFWFGHQGYEYVDLGRFW
                     QLFLFVGLWVWLILMGRAMWPAFKKPGEHRHLLALFLMASTAIALFYGAGLMWGRQTH
                     YALAEYWRWWVVHLWVEGFFEVFATVVIAFLFTRMGLLRTVTATAAVLFSTTIFLSGG
                     IIGTFHHLYFSGTPMAVMALGAVFSALEVVPLVLIGFEAYENLTLSRARRWVSAYRWP
                     IYCFVAVAFWNLVGAGLFGFLINPPIALYYMQGLNTTAVHGHTALFGVYGMLGIGLML
                     FCLKGLSVRRTWRTRPLAIAFWSINIGLALMMLLSDLPRGLMQTVASVNEGMWWARSA
                     DFMQQGIMETFRWLRAIGDTIFGVGVLVLGWFVLGIKTGWSLTDQPEAIAEQFTESAV
                     TQSARH"
     gene            1943..2290
                     /locus_tag="PKK20_07315"
     CDS             1943..2290
                     /locus_tag="PKK20_07315"
                     /inference="COORDINATES: protein motif:HMM:NF022731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3088 family protein"
                     /protein_id="HNU99733.1"
                     /translation="MTQDRLYLLKAGFIDQGKGPYFCPECAQMVGLLEFYPALKQRLE
                     VRWLNFPRPRPELIDLLGEENQNCPVLVLKEAPKGLPSKLALQEARGRWYVEGASDIA
                     AYLAHVHDIGIPH"
     gene            2604..4916
                     /locus_tag="PKK20_07320"
     CDS             2604..4916
                     /locus_tag="PKK20_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNU99734.1"
                     /translation="MKHITYPRAPIMHAPILILSLVLCAGPGTASDLILWYRQPAATN
                     KLMEEALPLGNGRMGCMLSGGIERERIQFNEQSLWSGDNNWDGEYECGDHGFGAYRHF
                     GDLTILWRNRGEDANAAKHADSSGANAPAPIAVPVDYRRALDIATGIHRTTFTRHGIA
                     FSREAFASRPHQVLVFRYTADKPGALSGRIGLESGQKATTETAPQCLSFAGRMPNTLR
                     HAAVVRLLHTGGTVAAAGREIVFDRCDTLTLLLDARTDYKPDYSAGWRGEDPLPRIEK
                     ELADAQTQSYDALRRAHIADLTSLIDRVALDIGATASDVLDQPTDARLKRYAAGHADP
                     QLESLMFCYGRYLLASCSRPGGLPANLQGLWNDSNTPPWASDYHNNINIQMNYWGAEP
                     THLPECHLPLIDFVVAAQEPCRIATRKAFGEKTRGWTARTSQNIFGGNGWEWNIPASA
                     WYAQHLFEHWAFTQNKDYLAQTAYPVLKEICHYWEDRLKSLPDGTLVAPNGWSPEHGP
                     REDGVMHDQQLIWDLFENYLDAAKALGVDADDQARIADLQARLAPNKIGRWGQLQEWQ
                     TDRDDPNDTHRHTSHLFAVYPGRQISPGRTPDLARAAIVSLKARSNDRGDKPFGVDTT
                     IGDSRRSWTWPWRGAMWARLGEGNRAGIMVRGLLTFNTLPNLFCNHPPFQMDGNFGIT
                     GAIAEMLLQSHAGEIAILPALPDAWARNGSFRGLKARGNFTVDATWKDGKVTHFAVRS
                     PEPRSVKVRVNGELKTLTTVAEQSVGVTKD"
     gene            5021..5899
                     /locus_tag="PKK20_07325"
     CDS             5021..5899
                     /locus_tag="PKK20_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNU99735.1"
                     /translation="MKTWMLWTGRGLALATLTTITSLNLLAQPAIPNEYKTGGFAIGC
                     QAYTFNRFSVFEAIEKTAHAGARVIEFYPGQKLSKEEPAVRWDHNASDEVVAKVKAKL
                     AEHRIMAVNYGVVDIPKDEAAARKIFEFAKTMGMRGITTESTDSIDTIEKLAKEYDLV
                     VGYHNHPRQPNNPNYRVWDPNYIVELTKGRDPRVGACVDTGHWLRSNLNPVECLKILK
                     GRIVSSHLKDLHQKGPGAHDVPFGTGVADIRACLDELAAQGFAGNISIEYEHNWDNSV
                     PEVAQCIGFVRGYGKP"
     gene            5949..>7219
                     /locus_tag="PKK20_07330"
     CDS             5949..>7219
                     /locus_tag="PKK20_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99736.1"
                     /translation="MEKHRPHTSLNRRQFLTRTTAAVGAASFAFPYIGHVQGANDRIN
                     IACIGVGGKGDSDSSDAANCGGNIVAICDIDQGTLDRKGKQFSKAKQYRDYRKLLDEM
                     GKEIDAVTVSTPDHNHGVAAIRAMKMGKHCFCQKPLVQNVKEARIMRQLATEKKLATQ
                     MGNQGSAESGLRRAVEVIQAGVIGKPIELHVWSNRPVWPQGYDRPAGEDPVPDGVDWD
                     AWIGPAAMRPYKKGIYHSFAWRGWYEFGTGALGDMACHTVNMPFRALKLGYPTLVECE
                     LASRVYPETFPETSRIRFEFPEREGLPPLKFWWYDGNPNGTPAVPGAPAREKIRPLRP
                     VAEVAKEIIATQGGLPGSGALIVGEKGKVFSPDDYGAQFYLAMKGEDEFKSSGQHEAC
                     KDVPQSIPRSPGHMQEWFRMMKDGTPAYSNF"
BASE COUNT         1428 a         2386 c         2101 g         1304 t
ORIGIN      
        1 cctgcgacgt gcccgtcttc ccggcccgcg gcatgcccgg ctcgacgatg cgggcgcgga
       61 aggcggtgcc gcgcggatcg ttgaccacgc cggtcatcgc cttctggatg atcgcgagat
      121 gcgcctgcgg gatgccgagc ggcgacatca gctcaccggc ttcttcttct gggccgcgtg
      181 ggcctgcgcc gctaaccgcc ccggagaaac cgtcagttac acccaaaact ggccgcccga
      241 accgcttgtc gccaacactc cgaccggttc gagcgttctg tggtcggtgg tgagtttcgt
      301 gttgctgctg gctggcatcg gcgcactggc gtggtacaac gccgcgctga aaggcagaga
      361 ggaacagccc cacgatctgc cggagaagga cccgttgctg gggctgcaac cgaccccttc
      421 catgcgcgcg acgctgaagt atttctgggt ggtggtcgcg ttgatcatgg cgcaaatcat
      481 tatgggtgtg gtgacagcgc actatggcgt ggaaggcaac ggcttctacg gcattccgct
      541 ggctgaatgg ctgccatatt cggtcacgcg cacgtggcac acgcaattgg ccgtgttctg
      601 gattgccacc gcttggctcg cgaccggact gttcatcgct cccgccgtct ctggccacga
      661 accgaagtat cagcgagccg gggtgaactt tctcttcgtc tgcctgctga tcatcgtcgt
      721 cggttcaatg ttcggaacgt ggtacggcac acgtcagcgc atgggctacg aaatgaactt
      781 ctggtttggc catcaaggct acgagtacgt ggatctagga cggttctggc agctctttct
      841 attcgtgggg ctgtgggttt ggctcattct catgggacgc gccatgtggc cggccttcaa
      901 gaagccgggc gaacaccggc atctgctggc gctgttcctc atggcctcga cagccatcgc
      961 gctcttctac ggcgccggcc tcatgtgggg acgccagact cattacgcgc tagccgagta
     1021 ctggcgctgg tgggtggtgc atctgtgggt ggaaggcttc ttcgaggtgt tcgccacggt
     1081 ggtcatcgcc ttcttattca ctcgcatggg actgttgcgc accgtgaccg ctacggcggc
     1141 ggtgctgttc tccacgacaa tcttcctctc cggcggcatc atcggcacgt ttcaccatct
     1201 gtacttcagt ggcacgccga tggcggtgat ggcgctgggg gcggtgttca gcgcgctgga
     1261 ggttgtgccg ctcgtcctca tcggttttga agcctatgaa aaccttaccc tctctcgcgc
     1321 gcggagatgg gtttcggcct atcgctggcc gatctactgc ttcgtggcgg tggccttctg
     1381 gaacctggtc ggcgcaggat tgttcgggtt tctcatcaac ccgccgattg cgctttacta
     1441 catgcaggga ctcaatacga ccgctgttca cggtcacacg gcgctgttcg gcgtttacgg
     1501 catgttgggc atcggcctga tgctgttttg cctgaaagga ctctccgttc gccgcacgtg
     1561 gcgcacgcga ccgctcgcga ttgcgttctg gtccatcaac atcggccttg cgctcatgat
     1621 gctgctgtcc gacctgccgc gcggcctgat gcaaaccgtg gccagcgtca acgaaggcat
     1681 gtggtgggcg cgctcggcgg atttcatgca acagggcatc atggagacat tccgttggtt
     1741 gcgcgcgatt ggcgacacca tcttcggcgt cggcgtcttg gtgttgggct ggttcgtgtt
     1801 gggtatcaag accggctggt cgctcaccga tcaacccgaa gcaatagcgg aacagttcac
     1861 cgaaagcgcc gtgacgcagt cggcgcgaca ctgatcccac gtccaagact cgctcctggc
     1921 atcgcactcg gacagaatta ccatgaccca agaccgattg tatctcttga aggccggttt
     1981 catcgaccag ggcaaaggcc catatttctg tccggagtgc gctcagatgg tcgggctgtt
     2041 ggagttctac ccggccctca aacagcgctt ggaagtccgc tggctcaatt tcccgcgccc
     2101 acgcccggag ttgattgacc tattgggcga ggaaaaccag aactgccccg tgctggtgct
     2161 gaaagaggct cccaagggac tgccctcgaa gctcgcactc caagaggcaa gaggtcgttg
     2221 gtacgtcgaa ggcgcaagtg acatcgcggc ctacctggcg cacgtacacg acatcggcat
     2281 tccgcattga tccaaccgcc gtgcccctga atgaaacaaa atttgctgga ggggatttgg
     2341 ctcgctggcg aggcgcgacg agggagcata cccgcagcgg tgtgtgagcg aagagcaatc
     2401 ccgcgtccgc gggaccagcg agccaaagcc ccccagccct tcggggcggg gcggcaccgg
     2461 accatctgcg ttgttcctcg gtcagtcgag tatctctggg gatacactcc gtcccttggg
     2521 cctcgcatct ggcccggtgg cgcttccgcc aaaaccgaaa gagattttcc acagacccta
     2581 agagttgcca gcccgaaagt cccatgaaac acattaccta tcctcgagcg cccataatgc
     2641 atgcgccaat cctcatcttg tcgttggtgc tctgcgcggg tccgggcacg gcatctgatc
     2701 tcatcctttg gtaccgccag cccgccgcca cgaacaagct catggaagaa gcgctgccgc
     2761 tgggcaacgg acgcatgggt tgcatgctct ccggcggcat cgagcgcgag cgaatccaat
     2821 tcaacgagca aagcttgtgg tctggcgaca acaattggga cggcgaatac gagtgcggcg
     2881 accatggatt cggcgcctac cgccatttcg gcgatcttac aatcctctgg cgcaatcgag
     2941 gcgaagatgc gaacgctgcg aaacacgccg attcctcggg cgcaaacgcc cccgcaccca
     3001 tcgcggtgcc cgtcgattac cgcagagcgc tcgacatcgc gaccggcatc catagaacga
     3061 ccttcacccg acacggcatc gcgttttccc gggaggcgtt cgccagccgt ccgcatcagg
     3121 tcctggtctt tcgctacacc gccgacaaac cgggtgcgct gtccggcagg atcggcctcg
     3181 agtcaggcca gaaagccaca accgagaccg ccccacaatg cctctccttc gccggccgca
     3241 tgcctaacac cctcaggcat gccgccgttg tccgtctgct ccacacggga ggcacggtcg
     3301 ccgcagccgg ccgtgaaatc gtgtttgacc gctgcgatac tctgacgttg ctgctcgatg
     3361 cgcgcaccga ctacaagccc gattacagcg ccggctggcg gggcgaagat ccgctgccgc
     3421 gcatcgagaa ggaactggct gacgcccaga cccaatccta cgacgccctg cggcgcgcgc
     3481 acatcgccga cctcacatcc ctcatcgacc gtgtcgccct cgatatcggc gccaccgcct
     3541 ccgatgttct cgatcaaccg accgacgcgc gcctcaaacg ctatgccgca ggacacgccg
     3601 acccgcagct cgaatcgttg atgttctgct acggacgcta tctgttggcc agttgctcgc
     3661 gccccggcgg cctccctgcc aacctccagg gactctggaa cgacagcaac actccgccct
     3721 gggccagcga ttatcacaat aatatcaaca tccagatgaa ttactggggc gccgagccca
     3781 cccaccttcc cgaatgccat ctcccgctga tcgacttcgt cgtcgccgcc caggaaccgt
     3841 gccgcatcgc cacccgcaag gcctttggtg aaaagacgcg gggttggacg gcgcgcacca
     3901 gccagaacat cttcggcggc aacggatggg aatggaacat tcccgcgagc gcctggtacg
     3961 cccagcatct gttcgagcat tgggcattca cacagaacaa ggactacctg gcccagaccg
     4021 catacccggt ccttaaggag atttgccact actgggaaga ccgccttaaa agcctgcccg
     4081 atggcacgct ggtcgctccc aacggttggt cgcccgaaca cggcccgcgc gaagacggtg
     4141 tcatgcatga ccagcaactg atctgggatc tcttcgagaa ctatctcgac gccgccaaag
     4201 ccctcggcgt cgatgccgac gaccaggcgc gcatcgccga tctccaggcc cggctggccc
     4261 ccaataagat cggacgatgg ggtcaactcc aggaatggca aaccgaccgc gacgacccca
     4321 acgatacaca ccggcataca tcgcatcttt tcgccgtcta ccccggacgc cagatcagcc
     4381 cgggccgaac gccggacctt gcccgggccg cgattgtctc gctcaaggcg cgcagcaacg
     4441 accggggcga caagcccttc ggcgtcgata caaccatcgg cgacagccgg cgctcgtgga
     4501 cctggccctg gcgcggcgcg atgtgggcgc ggctgggtga agggaaccgg gccgggatca
     4561 tggtgcgcgg cctcctcacc ttcaacacac tgcccaacct gttctgcaac caccctccgt
     4621 tccaaatgga tggcaacttc ggcatcactg gcgcaatcgc ggaaatgctc ctgcagtcgc
     4681 acgccggtga aatcgccatc ctgcccgcac tccccgatgc ctgggcccga aacggctcgt
     4741 tccgcggact taaggcccgc ggcaacttca ccgtggacgc cacctggaag gacggcaaag
     4801 tcacccactt cgccgttcgc tcgcccgaac cgcgatcggt caaggtccgc gtcaacggcg
     4861 aactcaagac gctcaccacc gtcgcggagc agtctgtcgg agtcacgaag gactgatttg
     4921 agtttgacac tcggggttga atcgcgctgc tatttcccca tacgcggcgg agcggcttga
     4981 gtttggcctt cgcagcggcg acacgaagaa tgaattaacc atgaagacct ggatgctatg
     5041 gacgggtcgc gggttggctc tggccacctt gacgaccatc acctccctca acctcttggc
     5101 ccaaccggcg attcccaacg agtacaagac gggcggattc gccatcggat gccaagccta
     5161 caccttcaac cgattcagcg tgttcgaggc gatcgagaag accgctcacg ctggagccag
     5221 ggtgatcgag ttctaccccg gccagaaact cagcaaagag gaaccggctg tccggtggga
     5281 tcacaacgcc tccgacgagg tcgtcgcgaa ggtcaaagcc aagctcgccg agcacagaat
     5341 catggcggtc aactacggcg tggtcgatat cccgaaggac gaagcagccg cccgcaagat
     5401 tttcgaattt gccaagacaa tggggatgcg cggcatcaca accgaatcga cggactcgat
     5461 cgacacgatc gagaaattgg ccaaggagta tgacctggtg gtcggttacc acaaccaccc
     5521 tcgccaaccc aataacccga attacagagt ctgggatccg aactacattg tggaactgac
     5581 caaaggacgc gatccgcggg tcggagcctg cgtcgacacg ggccactggt tgcgctcgaa
     5641 tctcaacccg gttgaatgcc tgaagattct caaaggccgc atcgtgagca gccatctcaa
     5701 agacctccat cagaaaggcc ccggcgccca cgacgtcccg ttcgggaccg gcgtggcgga
     5761 catccgggcc tgcctcgacg aacttgcggc ccagggattt gccggcaata tctcgatcga
     5821 atacgagcac aactgggaca acagcgtgcc tgaagtcgcc cagtgcatcg gcttcgtgag
     5881 aggctacggc aaaccttgat tcagcaacgg acaacccgcg cccaggcgcg atcacctgac
     5941 atcacactat ggaaaaacac agacctcaca cctcgttgaa tcggcgacag tttctgaccc
     6001 gcacaaccgc ggcggtcggc gccgcttcat tcgccttccc gtacatcggc cacgtgcagg
     6061 gcgcgaacga tcgcatcaac atcgcctgca tcggcgtcgg cggcaaaggc gacagcgaca
     6121 gcagtgacgc cgccaactgc ggcggcaaca tcgtcgccat ctgcgacatc gaccagggca
     6181 ccctcgaccg caaagggaag cagttctcaa aagccaaaca gtaccgcgac taccgcaagc
     6241 tgctcgatga aatgggcaag gagatcgacg ccgtcacagt ctcgactccg gaccacaacc
     6301 atggcgttgc cgccatccgc gccatgaaga tgggcaagca ctgcttctgt cagaagcccc
     6361 ttgtccaaaa cgtcaaggaa gcgcgcatca tgcgccaact ggccaccgag aagaaactcg
     6421 ccacccaaat gggcaaccag ggcagcgccg aatccggcct gcgccgggcc gtcgaggtca
     6481 tccaggccgg cgtcatcggc aaaccgatcg aactgcacgt ctggtcgaat cgcccggttt
     6541 ggccccaggg ctacgaccgc cccgccggcg aagaccctgt gcccgacgga gtggattggg
     6601 acgcctggat cggccccgcc gccatgcgcc cctacaagaa aggcatctac cactccttcg
     6661 cctggcgcgg atggtacgag ttcggcaccg gtgccctggg cgacatggcc tgtcacacgg
     6721 tcaatatgcc gttccgcgcc ctgaaactcg gctacccgac cctcgtcgag tgcgagctcg
     6781 cctcgcgcgt ctatccggaa accttccccg agacctcgcg catccgcttc gagttccccg
     6841 aacgcgaagg cctcccgccc ctcaagttct ggtggtacga cggcaatccg aacggcaccc
     6901 ccgccgtccc aggcgcgccg gctcgcgaga aaatcaggcc gcttcggccc gtcgctgaag
     6961 tcgccaagga aatcatcgcc acccaaggcg gcttgcccgg cagcggtgcc ctcattgtcg
     7021 gcgaaaaggg caaagttttc tccccggatg actacggcgc ccagttctac ctggccatga
     7081 aaggcgagga cgaattcaag tccagcggcc aacacgaggc gtgcaaggac gtgccgcagt
     7141 ccattccgcg ctccccgggc cacatgcagg agtggttccg aatgatgaag gacggcacgc
     7201 ccgcttactc gaacttcga
//
LOCUS       DAOQRO010000160         7141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10254_length_7141_cov_5.016653, whole genome shotgun sequence.
ACCESSION   DAOQRO010000160 DAOQRO010000000
VERSION     DAOQRO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10254_length_7141_cov_5.016653"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="PKK20_07335"
     CDS             <1..1710
                     /locus_tag="PKK20_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNU99737.1"
                     /translation="VGKRSVVKIRDFAQAEKDLEELQGVMTAAEGCEWGLWTNGLERF
                     FLRKEKRRFEVRFAPHGDWPMADGTMSTPDVHAETYVRPGDENALRRAFQRCHNFIHG
                     NEGMPKDAAFWQFLYLIFAKMYDERANRGPARKFFALASEPFTEEGRMKIRTRIDELF
                     KATKRHYGLFRDSDEITLSNRALAYMVMELARYNFSRSDVDAKGAAYQEIVGANLRGD
                     RGQYFTPRRAVNLVVRILDPKENEKVFDPADGTGGFLVATLAHQLRRFRAEYKRELGE
                     KTQDSILDRLRDYARTKVFGADFDPFLVRASTMNVLMAANIEGNIFHMDSLAFPHGHL
                     PGNELAKKHVPFGSVDVLMTNPPFGSEIPITEPAILQNHQLARRWTRTDDGQWMEQTT
                     TQNAVAPEILFIEQSIRWLKPGGRMGIVLPNGILGNPGDEYIRRWILRNCWVLACVEV
                     PVEAFIVEANVGILTSLLFLKKKTETEMDAEAQGHVKEYPIFMAVAEKAGVDRRGNPL
                     HKRNPDGTEKMVTRIYEERVKTNGNYETRQRKIIEPEIDDDFPAIGDAYEAFRAKNQE
                     PGA"
     gene            1707..3077
                     /locus_tag="PKK20_07340"
     CDS             1707..3077
                     /locus_tag="PKK20_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU99738.1"
                     /translation="MITRIEAYRYRCFERLDLALDRFQVFVGPNGAGKTTLLDIPIVL
                     GEMLAARSIEKAFFKETPSHPRQRTDYARDLIYTRAGNNFIMAVEARLPESIIAELVD
                     KQAKRLEKRKFSDSGRAVKALREKRDRWFSRIRYEIEFELFNEALQIGQEYLLLLSEG
                     ANTRKRAPSGLLGEWVSPGSRQIVPVLRRPRGGKVTFRPEGEARGPRNEYGFPVTEPA
                     FANVFADRSRYGASLWLRDLLTNFACAYQPSFAALRDPQPRIQQPVVVRDASTLAWLA
                     KDLEKPTDAEQAKSKAKLTRSRAFERWERLARLALPGLVRIEPFVRDDDKHAYLRLHY
                     RNGAVVPSSGLSDGTLTILALTILSFLPSTPALLTYEEPEDGVHPKGIEILLEALQTV
                     HGSQVFVSSHSPLVLARCQPSQIVCMSLDPKGAATAVRGDQHPALTEWKESVNLGTLL
                     AAGVLG"
     gene            3074..3802
                     /locus_tag="PKK20_07345"
     CDS             3074..3802
                     /locus_tag="PKK20_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99739.1"
                     /translation="MKPLLLFVADSECAGTLRGFFERKKFHTSLGCGPIELDGVSFNP
                     EQDIRVHPAHDPGMWTDPHTVLLAERNNFQHALLILDEAWEGAPPAEQIVSDIEGLVQ
                     SRALWQRERFEVILIRPELEAWIWQRNPHVAEAFEFSGTDGELWQFLAAQALRLDREK
                     KKHRFVPANPLAQLPPAWPTDQLKPANPKGLVEALSHHCQSGPASGIFNEISSTISLK
                     GCRDVAFLRLRDTLQQWFPKRREA"
     gene            3838..5220
                     /locus_tag="PKK20_07350"
     CDS             3838..5220
                     /locus_tag="PKK20_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99740.1"
                     /translation="MEGYGYRLDCSPYLGGALETKILLEKLPLRKDPLHTLTKGFDGG
                     IYNGPQFVRNYVESAEHGVPFMTGSSMQLADLSNLPMLSRRDAHGTKLRHLELQPGMV
                     LISCSGTIGKMAYARQDMKGIWSSQDVLKVVADPEKIPSGYLYAYLSSKFGVPLLVSG
                     TYGAIIQHLEPLHIAELSVPRLDDALEQEIHALVEEAAELRTKASQLKREAVAAFVRL
                     CRLPDLKVLNKYPTPHLGSATSTQMTQRMDSSYFIAPCAEARAAFDAAGRWWQSKSLG
                     AVAEVFIPTIFKRLYADDPAQGYPYITGADVFCIKPTSSQYLMRGVARENRLLLENGM
                     IVLHEAGQRYGLIGRGVMVGRTLEGFACTNNMVRLVPHDEAEAGYIFAALSSEHGICL
                     LKREAAGSSIPHLDAGRIASVEIPWPDKPIRAQVAELAHRARECWDRADELEEEADSK
                     LVRAIEHAAK"
     gene            5226..>7141
                     /locus_tag="PKK20_07355"
     CDS             5226..>7141
                     /locus_tag="PKK20_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein kinase family
                     protein"
                     /protein_id="HNU99741.1"
                     /translation="MAQVIHIGQPANDPEREAIGYLRDHLPGDYRVIHNFELRSDDGQ
                     WFEIDVAVVAPHAVYLVDVKSTYGEIHVAGGKWHPEGRPPFASPLAKLRQHTRQLHGV
                     LSAAAAPPHAALRHVWVEAVVLLTAPDAVLRDPEGRDRDNTWKLAGCERSFTDASLLP
                     QRTPPPAPTAPHLGHILSTLTGKARPQQGLPLLGRSWQCEERLTGNDFYTEYRARNAY
                     ASSPERVIVRVYRADPYLPAEERAAQRERIANAYQALTRLPPHPAIPAARDFFPTERD
                     DAYVLVLNDAPGSSLRVHLTKPGLQLTMDQKLRVTRDLLSALVHCHASGVLHRAISPA
                     TVVVGRDGQTRLLDFDFARPGPPRERTLAHEIPEVVEPAYLAPELKDDALKSSAASDV
                     YAAGATLYELFTGKPTFDKLDEALAATQVFPEKVSSLVGGLPGEFDAWLESLCRFTPT
                     ERPTAAAALSGFDALFAPPPALTSESEPEPPPPGTPLDYANLQPGVLLKSKYQVEAAL
                     GKGGFGRVYKVIDTFGDVTRALKIITVDRSSVAERMKQEYRTLANLPEHPGVVKVYDG
                     DFLEGDRVPFLVMEFVEGANVGDLINDKKISVAEAHQMGIGVAEALGHLHRHRVTHGD
                     IKPANLMWTSKAVR"
BASE COUNT         1413 a         2198 c         2210 g         1320 t
ORIGIN      
        1 gtcggcaagc gctcggtggt gaagattcgc gacttcgcgc aggcggagaa ggatttggag
       61 gaacttcagg gcgttatgac cgctgccgaa gggtgcgagt gggggttgtg gaccaacggc
      121 ctggagcggt tcttcctgcg caaggaaaag cgccgatttg aagtccggtt tgcgccgcac
      181 ggggactggc cgatggcgga cggcaccatg agcacgccgg acgtccatgc ggaaacctac
      241 gtccgccccg gcgacgaaaa cgccctgcgc cgagcattcc agcgttgcca caatttcatc
      301 cacggtaacg agggcatgcc caaggacgcc gccttttggc aattcctcta cctcattttc
      361 gccaagatgt atgacgagcg ggcgaaccgt ggccctgccc gcaagttctt cgccttggcc
      421 agcgagccgt tcacggaaga ggggcggatg aaaattcgca cccgcattga cgaactgttc
      481 aaggcgacga agcggcacta tggcctgttc cgtgattcgg acgaaatcac gctctccaac
      541 cgtgccctgg cctacatggt catggagttg gcccgctaca acttctcacg gtcggacgtg
      601 gacgcgaaag gggccgccta tcaggaaatc gtcggcgcga atcttcgcgg cgaccggggc
      661 cagtatttca ctccgcgccg ggcggtgaat ctggtcgttc gcatccttga cccgaaggaa
      721 aacgagaagg tgttcgaccc tgcggatggc acgggcgggt tccttgtggc cacgctcgcg
      781 catcaactcc gccggttccg tgccgaatac aaacgcgaac tcggcgagaa aacacaggac
      841 tccattctcg accgactgcg cgattacgcg cgcacgaagg tcttcggtgc ggactttgat
      901 ccgttcctgg tgcgcgccag caccatgaac gtgctcatgg ccgcgaacat cgagggaaac
      961 atcttccaca tggattcgct ggcgtttccg cacggccatc tgccgggtaa tgaacttgcc
     1021 aagaagcatg ttccgttcgg ttccgtggat gtgctgatga cgaatccgcc gttcggttcc
     1081 gaaattccca tcacggagcc ggccattctc cagaatcacc aactcgctcg ccgctggacg
     1141 cgcaccgacg acggccagtg gatggagcaa accaccacgc agaatgcggt cgcgccagaa
     1201 atcctgttca ttgagcagtc catccgctgg ttgaagcccg gtgggcgcat gggcatcgtc
     1261 ttgcccaacg gtatcctcgg caatccgggc gacgaataca tccgccgctg gattctgcgg
     1321 aactgctggg tgctggcctg cgtggaagtg ccggtggaag ccttcatcgt ggaggccaac
     1381 gtcggcattc tcaccagcct gttgtttctc aagaagaaga ccgagaccga gatggacgcc
     1441 gaggcgcagg gacatgtgaa ggaatatccc atcttcatgg cggtggcgga aaaggctggc
     1501 gtggaccggc gcggcaaccc gctgcacaag cgcaaccccg acggcacgga gaagatggtc
     1561 acccgcattt acgaggaacg ggtcaagacc aacggcaact acgaaacccg gcaacggaag
     1621 atcatcgagc ccgagattga cgatgacttc ccggccatcg gcgacgccta cgaggcgttc
     1681 cgcgccaaga accaggagcc aggcgcatga ttacgcgaat cgaagcctac cgttaccgct
     1741 gctttgagcg gctcgatcta gccctcgacc ggtttcaagt tttcgtcggc ccaaatggag
     1801 caggcaaaac caccctcctg gacattccga ttgtcctcgg ggaaatgctc gcggcgcgga
     1861 gcatcgagaa agccttcttc aaggagactc catcgcatcc gcgccagcgc acggactacg
     1921 cgcgggatct catctacacg cgcgccggca acaatttcat catggcggtg gaagctcgct
     1981 tgcccgaatc catcattgcc gaactcgtgg acaagcaggc gaaacgcttg gagaagcgca
     2041 agttcagcga cagcggacgc gcagtgaagg ccctgcgaga aaagcgcgac cgctggttct
     2101 cgcgcattcg ctacgaaatc gagttcgagt tgttcaacga agcgctgcaa atcggacagg
     2161 aatacctact gctcctttcc gagggggcca atacgcggaa acgcgctccg agcggacttc
     2221 tgggtgaatg ggtctcccct ggttcccggc agattgtgcc ggtgctgcgc cgtccgcgag
     2281 gcggcaaagt cacgttcagg cccgagggtg aggcgcgtgg cccgcgcaac gaatacggtt
     2341 tcccagtcac ggagccagcc tttgccaacg tcttcgccga ccggagccgc tatggcgcat
     2401 ccctctggct gcgcgacctg ctgaccaact tcgcctgcgc ctaccagccc tcgttcgcgg
     2461 ccctccgcga cccgcaaccg cgcattcagc aaccagtcgt cgtgcgcgat gcctcgacct
     2521 tggcgtggct ggcaaaagac ctggaaaagc caaccgacgc ggaacaagcc aaatcgaaag
     2581 caaaactcac gcgcagtcgt gctttcgagc gatgggaacg cctcgcgcgc ctggccttgc
     2641 ccggcttggt ccgaatcgag ccgttcgttc gcgacgacga caagcacgct tacctgcggc
     2701 ttcactatcg caacggggct gtggtgccgt cgtccgggct gtcggatgga acgctcacaa
     2761 ttttggcgct caccattctc tcgttcctgc cgtcaacacc ggctctgctg acctacgaag
     2821 agccggagga tggagtccat ccgaagggaa tcgaaatcct gctggaagcg cttcaaaccg
     2881 tccacggctc gcaggttttc gtctcgtcgc attcgccgct cgtcctggcc cggtgccagc
     2941 cgtcgcagat tgtctgcatg agcctcgacc cgaaaggggc ggcgactgcg gtgcgcggcg
     3001 accagcatcc ggcgctgacc gagtggaagg aatccgtcaa ccttgggacg cttctcgcag
     3061 cgggggtgct cggatgaaac cgctcctcct cttcgtggcg gacagcgagt gcgccggcac
     3121 actccgcggc ttctttgagc gcaagaaatt ccacacctcc ctgggttgtg gccctatcga
     3181 gctagacggt gtcagcttca atccagaaca ggacatccgg gtgcatcccg ctcacgatcc
     3241 gggaatgtgg accgacccgc atacggttct cttggccgag cgcaataatt tccagcacgc
     3301 cctgttgatt ctggacgaag catgggaggg ggcgcctccc gccgaacaga ttgtgtccga
     3361 tatcgaaggc ttggtgcaga gccgtgctct ctggcagcgg gagcgattcg aagtcatcct
     3421 gattcgacca gaactcgaag cctggatttg gcagcgcaac ccgcatgtgg cggaggcctt
     3481 cgagttctcc ggcacggatg gcgagctttg gcagttcctc gcggcgcagg cattgcgctt
     3541 ggacagggag aagaagaagc accgctttgt tcctgccaat ccactcgctc aattgccgcc
     3601 ggcttggccc acggaccaac tcaaacctgc gaatccgaag ggcttggtcg aggcgctgtc
     3661 gcatcactgc cagagcggac ccgcgtccgg catcttcaac gaaatcagtt caaccatcag
     3721 cttgaagggg tgcagggacg ttgccttcct tcgactgcgc gacacactcc agcagtggtt
     3781 tcccaagcga agggaggcgt gagagtagtg agaaccgccg tcattcgcag ttcgtggatg
     3841 gagggctacg gctaccggct tgattgctcc ccctacctcg gcggcgcgct cgaaaccaaa
     3901 atcctgctcg aaaaactccc gctccgaaag gacccgctcc acacgcttac caaaggcttt
     3961 gacggtggca tctacaatgg gccacagttc gtccgcaact atgtggagtc ggcggagcat
     4021 ggcgttccgt tcatgacggg tagttcgatg caactcgcgg acttgtcgaa cctgccaatg
     4081 ctgagcaggc gtgacgctca tgggacaaag ttgcgccatc tcgaactcca gcccggcatg
     4141 gtgctgattt catgttctgg cactatcggc aagatggcct acgcaaggca ggacatgaaa
     4201 ggaatctggt cgagccagga cgtgctcaag gtggttgccg acccggagaa gattccttct
     4261 ggctacctct acgcatacct aagcagcaaa ttcggcgtgc cactcctcgt gtctggcact
     4321 tacggcgcca tcattcagca ccttgaaccg cttcacattg ctgagttgtc agtcccacgt
     4381 cttgacgatg ccttggaaca ggaaatccac gccctcgtcg aggaagcggc ggagttgcgg
     4441 acgaaggcga gtcaactcaa gcgtgaagcg gtagccgcat ttgtccgact ttgccggctt
     4501 ccagacttga aggtgctgaa taagtatcca actcctcatc ttgggtcagc tacttcaacg
     4561 cagatgacac agagaatgga cagcagctac ttcattgcgc catgcgccga agctcgtgca
     4621 gccttcgacg cagccggccg ctggtggcaa tcaaaatcac tcggagcggt ggccgaggtg
     4681 tttattccga ccattttcaa gcgactttac gctgatgacc cggcacaggg atacccatac
     4741 atcaccgggg ccgacgtgtt ctgcatcaag ccaaccagca gccaatatct gatgcgcggc
     4801 gttgcgcgtg aaaaccggct cttgcttgag aacggaatga ttgtgcttca cgaagctggt
     4861 caacgatacg gtctcattgg gcgaggagtg atggtcggtc gcacgctgga aggctttgcc
     4921 tgcacgaaca acatggtgcg ccttgttccg catgatgagg cagaagccgg ctacattttt
     4981 gccgctctgt cgagtgaaca cggcatctgc ctgctcaaac gcgaggcggc ggggtcttcg
     5041 attccgcatc ttgacgccgg ccgcatcgcg tcggtggaga ttccttggcc agacaagccg
     5101 attcgtgccc aggttgctga gcttgcccac cgagctcgcg aatgctggga ccgggccgac
     5161 gagttggaag aggaagctga ctcgaagctg gtgagggcca tcgaacacgc ggcgaagtga
     5221 ctgctatggc ccaagtcatc cacattggcc aaccagcgaa cgaccccgag cgggaggcca
     5281 ttgggtatct gcgcgaccat ctgccgggtg attaccgcgt catccacaac ttcgagctgc
     5341 gcagtgatga cgggcagtgg ttcgagattg acgtggcggt ggtggcgcca cacgcggtct
     5401 acctcgtgga tgtgaagtcc acctacggcg aaatccacgt ggccggcggc aagtggcatc
     5461 cggagggccg tccaccgttc gcgtcgccgc tggccaagtt gcgacaacat acgcggcaat
     5521 tgcacggagt gctgtccgct gccgccgcgc cgccccacgc cgccttacgg cacgtctggg
     5581 tggaagccgt ggtcctgctc accgcgccgg acgccgtctt gcgcgacccg gaagggcgcg
     5641 accgggacaa cacctggaag ctggccggct gcgagcggtc gttcaccgat gcctcgctct
     5701 tgccgcaacg cacgccgccg ccggcgccca ccgcgccgca cttgggtcac attctctcca
     5761 cgctcaccgg caaggcgcgt ccgcaacagg ggttgccgct gctgggccgg tcctggcagt
     5821 gcgaggagcg gctgacggga aacgacttct acacggaata ccgcgcccgc aacgcctacg
     5881 cttccagccc ggagcgggtc atagtccgcg tctatcgcgc cgatccatac ctcccggcgg
     5941 aagagcgggc agcgcagcgg gagcggattg ccaatgccta ccaagcgctg acgcggctgc
     6001 cgccgcatcc ggccatcccg gcagcgcgcg acttctttcc gacggagcgg gacgacgcct
     6061 acgtgctggt gttgaacgac gcgccaggct catcgctccg cgtgcatctg accaagccgg
     6121 gtctgcaact gacgatggac cagaagctgc gggtcacgcg tgacctgttg tcggcgctgg
     6181 tgcattgcca tgccagcggc gtcctgcatc gcgccatctc acccgccacg gtcgtggtgg
     6241 gacgcgacgg ccagacgcgc ctgctcgatt tcgattttgc ccggcccggc ccgcctcgcg
     6301 agcggacgct ggcgcatgag attccagagg tcgttgaacc ggcctacctc gcgccggaac
     6361 tgaaggatga cgcgctcaag agcagcgcgg cctcggacgt gtatgccgcc ggggcgacgc
     6421 tctacgagtt gttcacgggc aagccgacgt tcgacaagct cgacgaggca ttggcggcga
     6481 cgcaggtttt cccagagaag gtttcgtcct tggttggtgg attgccgggc gagttcgatg
     6541 cctggctgga atcgctttgc cgtttcacgc cgacggaaag gcccacggca gcggcagcct
     6601 tgtcgggctt cgatgccctg ttcgctccgc cgccagccct gacctcggag tccgagccgg
     6661 aaccgccgcc gccaggcacg ccgttggatt acgcaaatct ccagccgggt gtgttgctga
     6721 agagcaaata tcaggtggaa gcggcgttgg gcaaaggcgg cttcgggcgc gtctataaag
     6781 tcattgatac gtttggcgac gtgacgcggg cgctcaaaat catcacggtg gatcggagtt
     6841 ccgtcgcgga gcggatgaag caggagtatc gcacgctggc gaacctgccg gagcatccgg
     6901 gcgtggtgaa ggtgtatgac ggcgatttcc tggaaggcga ccgcgtgccc ttcctggtga
     6961 tggaattcgt cgagggcgcg aacgttggcg acctcatcaa cgacaagaag atttccgtgg
     7021 ctgaggcgca tcagatgggc atcggcgtgg cagaagcgct ggggcacctg caccgtcatc
     7081 gcgtgacgca tggtgacatc aagccggcga atctgatgtg gaccagcaag gcggtgagaa
     7141 t
//
LOCUS       DAOQRO010000161         7109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10325_length_7109_cov_4.497023, whole genome shotgun sequence.
ACCESSION   DAOQRO010000161 DAOQRO010000000
VERSION     DAOQRO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10325_length_7109_cov_4.497023"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..2569
                     /locus_tag="PKK20_07360"
     CDS             38..2569
                     /locus_tag="PKK20_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNU99742.1"
                     /translation="MTRREFLKLSAPAVGALGGAGAAAATAATPETNPPARRRSKYDA
                     EYARERLNRIAFPLGGLGAGMICLEGTGALSHVSLRHRPEVFHEPMTFAAICVKGSPN
                     VARVLEGPVPAWKLFGAPGTGNGAAGSSFGLPRFRQAAFRARFPFATVRLSDPDVPLR
                     VEVTGWSPFEPGAADDSSLPVAALEYRFYNPTRAPIEAVFSFNARNFMTVGSNANAVK
                     ATPGGFILWSAAAQDKAWEEGAFSATVTEPDAKVNHAWFRGGWWDALTMAWNDVRNGV
                     CQERPPVTEGGASPGATVFVPIRLAPRAAHTVTLRLAWHVARTNLRFGKDPDGIAPPT
                     DTHQPWYSKRFADIHEITRHWRDHYDDLRLRTKRFSDCFYDSTLPPEVIEAVAANLTI
                     LKSPTVLRQSDGRLWCWEGCGDNSGCCAGSCTHVWNYAQVIPHLFPALERTLRETEFG
                     PSQDDRGHQQFRSALPIRPLTHDFHAAADGQLGGIMKVHRDWRISGDTEWLRGLWPKI
                     KRSLDYCIATWDPDHKGWLEEPHHNTYDIEFWGPDGMCTSFYLGALQSAVRMGRALGD
                     EVPLYAELLARGTQRADTELFDGEYYIQRIEWKNLKAKNPLEVQSFHSGYSPEARLLL
                     EREGPKYQYGRGCLSDGVLGSWLAAVCGAGQVLDPDKVRSHLRAVHRYNLKHDLSAHA
                     NPQRPSFACGAEGGLLLCTWPKGGELSLPFVYSNEVWTGIEYQVASHLMLMGLVDEGL
                     EIVRVCRDRYDGRIRNPFNEYECGHWYARAMASYGLLQGLCGARYDAVDKTLHLEPSI
                     KGDFRCFLATATGYGTVGVKKGKPFVEVKAGAIDIQGGIRFKARA"
     gene            2595..3731
                     /locus_tag="PKK20_07365"
     CDS             2595..3731
                     /locus_tag="PKK20_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNU99743.1"
                     /translation="MGPALQQLDRVFVRYQGRTLVYFGGCDYFRLAFHPGVLAAARDA
                     TVRYGLNVAASRATTGNHSIYAELENELVRFFEVDRALLTPSGYATNLVAAQGMASHV
                     THALIDEQAHASLDDAAELLGCQVLRFRHLSPSDLAERLRRLPDAARPLVLTDGLFAR
                     EGTIAPLADYLRLLPRNGLLLVDDAHGAGILGSSGRGTPEICGVRRENRLIQTCTLSK
                     AFGTYGGAILGNASLIGRIVRQSRQFAGSTPLPPPLAAAALKAVQIVRSDPGLRSRLA
                     DRVRLVKDALCKTALAQPQTPSPVIAVQARTVPAAARLRKDLLRRGIYPSLIRYPGGP
                     PRGFFRFALSSEHTHKQLDALTASLLSVSGLAALGENNTTQRRT"
     gene            complement(3862..4197)
                     /locus_tag="PKK20_07370"
     CDS             complement(3862..4197)
                     /locus_tag="PKK20_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNU99744.1"
                     /translation="MTVETIWSRLTRMVFFLVLAASAMLVGLWYLPLIDENKAMRERL
                     FRIEVEVRREEAKAQQKRATIESLRTDPKAIERLAREQLGLAKPGETVIHFETPATNT
                     PGIVLPDYP"
     gene            complement(4279..5118)
                     /locus_tag="PKK20_07375"
     CDS             complement(4279..5118)
                     /locus_tag="PKK20_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNU99745.1"
                     /translation="MFCLQLRNELWKLFGKKRTYIGFFMFLFAQNVIALVFRFTQASK
                     PMQRMLEMSGHLAEPFISVLTIATIMLVPAAAFLLPLYVALVGGDLVAKEAEDGTMRM
                     ILSRPVSRFRLLVVKWVAGCIFSLLLVLALGVFGLAFSRLYFPWGGMFVWLPEHGIFG
                     VFEAQTGLVRYVAGHLMLVTEAASIMCLALLFSCFNVKPAAATILALSLTFVSFIMEH
                     IPYFKDYQNWFFTYHLHLWQFMFAEKIPWWRIGQSLSLLVGFNATFLAIGMASFHIRD
                     IKS"
     gene            complement(5133..6002)
                     /locus_tag="PKK20_07380"
     CDS             complement(5133..6002)
                     /locus_tag="PKK20_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99746.1"
                     /translation="MTKRFRGRLAVDDLTLEVPAGEIYGLLGHNGAGKSTAIGMMLGQ
                     VWPTRGEVRVCGHDVMLRRTLALRQVGAIFESPAFYDYLSGWRNLEILSHYTAPTSRQ
                     RLQEVIDWVGLTGRERSIVRTYSHGMRARLALAQALLPQPTLLILDEPGDGLDPEGIH
                     EMRETILRLHRELGLTILLSSHLLNEVEQLCTQIGVIRKGRKVFEGPTAHALQSRNWL
                     RITVPDFAAADRFLRQEGLIDESRDGRCVAMSEGIRSDVIIRRLVEGGFQVSEVARDE
                     QTLEDFYLSLMRR"
     gene            complement(6080..6634)
                     /locus_tag="PKK20_07385"
     CDS             complement(6080..6634)
                     /locus_tag="PKK20_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99747.1"
                     /translation="MNARLQTTAFTLIELLVVIAIIGVLASLLLPTLSKAQEAGRSTA
                     CLGNLRQIGIALQLYTQDNNNRLPEMRDRLFETNGVSSGDSQATIDVVLTNHLGSSQV
                     LKCPSDFRRVYEQTRSSYSWNSLLNGQDAEHLRVFGIDFDPHRIPLVFDKEAFHAAHG
                     AKRGVNYLYADGHIKNLLAIEGTK"
     gene            complement(6631..>7109)
                     /locus_tag="PKK20_07390"
     CDS             complement(6631..>7109)
                     /locus_tag="PKK20_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99748.1"
                     /translation="LTGEARAKALRELASKLNALSYEERQRARLDQEWSRWLLAMTET
                     EKSEIIEATLPTGFKQMLGAFEQLPVDRRRRTVDEAVKRLKEARQELAARDLPMPEWE
                     TNAPPVLSEEVRQKLITTGLKSFYSESSAQTKAEAAPLLEELQHMMESGVFLRGGR"
BASE COUNT         1327 a         2388 c         2099 g         1295 t
ORIGIN      
        1 ccaagccatg tctgagacac ccggtacgga aaacactttg acacgccgcg agtttctgaa
       61 gctctccgcg ccggccgttg gcgcgcttgg tggtgcgggc gccgcggcgg cgacggcggc
      121 aacgcccgag acgaatccac ctgccaggag aagatcgaaa tacgatgccg agtacgcgcg
      181 cgagcggctc aaccgcatcg ctttccctct cggcggcctg ggcgccggca tgatctgcct
      241 cgaaggcaca ggggctttgt cgcacgtgtc gctgcggcat cgtccggagg tctttcacga
      301 gccgatgaca ttcgctgcca tctgtgtgaa aggctcgccg aacgtggcgc gcgtgctcga
      361 aggccccgtg ccggcctgga agctgttcgg cgcccccggc accggcaacg gggccgcggg
      421 ttcctcgttc ggacttccgc gttttcgcca ggccgcgttt cgagcgcgct tcccgttcgc
      481 gaccgttcgt ctgtcggatc cggacgtccc gctgcgcgtc gaagtcacgg gttggagtcc
      541 tttcgagccc ggggccgccg atgactcgag cctgccggtc gccgcgctcg agtaccggtt
      601 ctataatccc acccgcgcgc ccatcgaggc tgtcttctcg ttcaacgcca ggaatttcat
      661 gaccgtggga agcaatgcca acgccgtcaa ggcaaccccg ggcggattca ttctctggag
      721 cgctgcggct caggacaagg cctgggagga gggcgccttc tcggctacgg tcaccgagcc
      781 cgacgcgaag gtcaatcatg cctggtttcg gggcggatgg tgggatgcgc tgacaatggc
      841 ctggaatgac gtccgaaacg gcgtgtgcca ggaacgtccg cctgtgaccg aaggcggcgc
      901 ttcgcccggc gcaacggtgt tcgtgccgat ccggcttgcc ccccgcgccg cgcacacggt
      961 gacgctgcga ctggcctggc atgtggccag gacgaatctg cgcttcggca aggatccgga
     1021 cggcatcgcc ccgccaaccg atacgcatca gccttggtac tcgaaacgtt ttgccgacat
     1081 ccatgagatc acgcgtcatt ggagggacca ctacgacgat ctgcgcctcc ggaccaagcg
     1141 tttcagcgac tgtttctacg attcgaccct gcctcctgag gtgatcgagg cggtggccgc
     1201 caacctgacc atcctgaaat cgccgacggt gctccggcag tcggacggtc ggctctggtg
     1261 ttgggaaggg tgtggcgaca actccggatg ttgtgccggc tcctgcaccc atgtctggaa
     1321 ctacgctcag gtgattccgc acctcttccc cgccctcgaa cggacactgc gcgagaccga
     1381 gttcggacct tcccaggatg accgtggtca tcagcaattc cgttcagccc tgccgatccg
     1441 gcccttgacc catgacttcc atgctgccgc tgacggtcag ctcggcggca ttatgaaggt
     1501 gcaccgtgat tggcgcatca gcggcgatac cgagtggctg cgcgggcttt ggcccaagat
     1561 caaaaggagt ctcgactact gcatcgccac ttgggatccg gaccacaagg gctggctcga
     1621 agaaccccac cacaacacct acgacatcga gttctggggg ccggacggca tgtgcaccag
     1681 tttctacctc ggcgcgctcc agtccgccgt gcgcatgggc cgtgcgctgg gcgacgaggt
     1741 tcccctctac gccgagttgc tcgcccgcgg gactcagcgt gccgacaccg agcttttcga
     1801 cggcgaatac tatatccaga gaatcgaatg gaagaacctc aaggccaaga acccgctcga
     1861 agtccagagc ttccacagcg gctattcacc cgaagcgcgg ctgctcctcg aacgcgaggg
     1921 acccaagtac cagtacggac gaggctgcct gtccgacggc gttctgggtt cctggctcgc
     1981 cgcggtctgc ggcgcgggcc aagtcctcga cccggacaag gtgcgcagtc atttgcgcgc
     2041 cgtccaccgc tacaacctca agcatgacct cagcgcccat gccaatccgc agcggcccag
     2101 ctttgcctgc ggcgctgagg gcggcctgct gctgtgcacc tggcccaagg gcggcgagtt
     2161 gtcgcttccg ttcgtctact cgaacgaggt ttggaccggc atcgagtacc aggtcgcgtc
     2221 ccacctcatg ctcatgggcc ttgtcgacga aggtctcgag atcgtgcgtg tctgccgcga
     2281 ccgctacgat ggacgcattc gcaatccgtt taacgagtac gagtgcggcc actggtatgc
     2341 gcgggcgatg gcgtcctacg gcctcctcca gggcctttgc ggagcccgct atgacgcagt
     2401 cgacaagacc ctccacctcg agccctcgat caagggagat tttcgttgct ttctcgccac
     2461 cgcaaccggc tacggcaccg tcggcgttaa gaaaggcaaa cccttcgtcg aggtcaaggc
     2521 cggcgctatc gacatccagg gaggaatccg gtttaaagcc cgcgcatgag tcgttccagg
     2581 ccctgagccc gatcatgggg cccgccctgc aacagctcga tcgcgtgttt gtccgctatc
     2641 agggacgcac cctcgtctac tttggcggat gcgattattt ccgcctggcc tttcacccgg
     2701 gcgttttggc cgcagcccgc gatgccaccg ttcgctatgg tctcaatgtg gccgcctcgc
     2761 gggccaccac gggcaaccat tccatctacg ccgaactcga gaacgagttg gtccgctttt
     2821 ttgaggtcga tcgcgcactg ctgacaccca gcggctatgc caccaacctc gtcgccgccc
     2881 agggcatggc cagccatgtc acccatgccc tcatcgacga acaggcccat gccagccttg
     2941 acgacgccgc ggagttgttg ggctgtcaag tcctccgctt ccgccatctc agcccctcgg
     3001 acctggccga gcgcttgcgg cggctccccg acgcagcccg tccgttggtg ctcaccgatg
     3061 gactcttcgc gcgcgaagga acgattgccc cattggccga ctatctgcgg ctcctgccac
     3121 gcaatggctt gttgctcgtg gacgacgccc acggcgctgg gattctggga tcgagcggac
     3181 gcgggacccc cgagatctgc ggggtgcgcc gggaaaaccg cctcattcag acctgcacgc
     3241 taagcaaggc cttcggcacc tacggcggcg cgatccttgg gaatgcctcc ctcattgggc
     3301 gcatcgtccg ccaaagccgc cagtttgcgg gcagcacccc tctcccgccg ccgctcgccg
     3361 ccgcggcgct taaagccgtg cagatcgtgc gatccgatcc aggcctccgt tccagattgg
     3421 cggatcgggt gcgcctggtc aaagacgcgc tgtgcaaaac ggctctggcc cagccccaga
     3481 cacccagccc cgtcatcgct gtccaggctc gcaccgtgcc cgcggcggcc cgtctgcgaa
     3541 aagacctgct ccgccgtggg atttacccca gcctcattcg gtatccgggt ggaccgccgc
     3601 gcggcttttt ccggttcgcg ctgtccagtg aacacactca caaacaactg gacgcgctga
     3661 ccgccagcct gctttcggtg tctggactcg cggcgctggg agaaaacaac acgacccagc
     3721 ggaggacctg acaaggccgc acagtggtcg ggacttggtg aaccaggcgg tttggacggc
     3781 ctgggtcgac gcagactcgg tctccaaccg acagccgtgc ttccggcaag ctgaggcggt
     3841 gtggctgccg atcgagaggg atcaagggta atcgggcagg acaatgccag gcgtgttggt
     3901 ggcgggggtt tcgaagtgga tgaccgtctc cccgggtttc gccaggccga gctgttcgcg
     3961 cgccagccgt tcgatcgcct tgggatcggt gcgcagcgac tcgatggtgg cgcgcttctg
     4021 ctgtgctttc gcctcctccc gccgcacctc gacttcgatg cgaaacaacc gctcgcgcat
     4081 cgccttgttc tcgtcgatca acggcagata ccacaatccc accagcatcg ctgaggccgc
     4141 cagcaccaga aaaaagacca tccgtgtcag gcggctccaa atggtctcca cggtcatgag
     4201 acagtcttct accttgcaga gcgcaaaagc ggaagcgcaa aaggcagcgc cggcggaaag
     4261 cgggaaggcc gtgtcgactc acgacttgat gtcgcggatg tggaaggagg ccatgccgat
     4321 ggccaggaag gtggcattga agccgaccag aaggctcagc gattggccga tccgccacca
     4381 agggatcttt tcggcaaaca tgaactgcca cagatgcagg tgataggtga agaaccagtt
     4441 ctggtaatcc ttgaagtagg gaatgtgctc catgatgaaa ctgacgaatg tcaacgagag
     4501 cgccaggatg gtcgcggccg ccggtttcac gttaaagcag gagaacagaa gggccaggca
     4561 catgatcgat gcggcctccg tcaccagcat cagatggccc gccacatacc ggaccagccc
     4621 cgtctgtgcc tcgaaaacac cgaaaatgcc gtgctccgga agccagacga acatgccacc
     4681 ccacgggaaa tagagccgag aaaaggccag tccaaacacg cccagcgcca gcaccagcag
     4741 cagggaaaaa atgcagcccg ccacccactt caccaccaac aaacgaaaac gcgacaccgg
     4801 gcgcgaaaga atcatccgca tcgtgccgtc ctccgcctcc ttggccacga gatctccgcc
     4861 caccagggcc acatagagag gcagcagaaa cgccgcggcc ggcacgagca tgatcgtggc
     4921 gatggtcagt accgagatga acggctcggc cagatgcccg ctcatctcga gcatcctctg
     4981 catcggcttc gaggcctgtg tgaaccgaaa caccagtgca atcacgttct gggcgaaaag
     5041 gaacatgaag aacccgatgt aggtccgctt cttgccgaac agcttccaaa gttcgttgcg
     5101 cagttgcagg caaaacatgg gcggaatccc gatcaccgtc tcatcaaaga cagataaaag
     5161 tcctcgaggg tctgctcatc acgcgccact tcggatactt gaaagccgcc ttcaaccagt
     5221 cggcggatga tcacgtccga tctgatcccc tcgctcatcg cgacgcagcg cccgtcacgg
     5281 ctctcgtcaa tgagtccctc ctgccgcagg aaccgatcgg cggcggcgaa gtcgggcacc
     5341 gtgatgcgca gccagttccg gctctgcagg gcgtgggcgg tcggcccctc gaacactttg
     5401 cgcccctttc ggatcacgcc gatctgggtg cagagctgct cgacctcgtt cagtaaatgc
     5461 gacgaaagca gaatcgtcag ccccagctcc cgatgcagcc tcaaaatggt ctcgcgcatc
     5521 tcgtggatgc cctccggatc cagaccgtcc cccggctcgt ccagaatgag cagcgtcggc
     5581 tgtggcaaca acgcctgcgc gagcgccaga cgcgctcgca tcccatgcga gtaggtgcgg
     5641 acgatcgagc gctcccgccc cgtcaacccg acccagtcga tgacctcctg cagccgctgc
     5701 cgtgatgtgg gagccgtgta gtgactcagg atttcgagat tcctccagcc gctcaaataa
     5761 tcgtaaaacg ccggcgactc gaaaatcgcc cccacctgcc gcaatgctag cgtccgccgc
     5821 agcatgacgt cgtgaccaca cacgcgcacc tccccccgcg tcggccacac ctgtcccaac
     5881 atcatgccga tggccgtgct tttccccgct ccgttgtgtc ccaacagccc gtagatctcc
     5941 cccgccggca cttcgagagt gagatcgtcc accgcaagac gcccccggaa tcttttcgtc
     6001 accccgcgca gctcgatgag tcgattcgat ctcggagacg cgccctccgc aaaggaggat
     6061 ccggacggcc cggtgactct cattttgttc cttcgatcgc caggaggttc ttgatgtgcc
     6121 cgtcggcgta caggtaattc acgccccgtt tcgcgccgtg cgccgcgtga aaagcctcct
     6181 tgtcgaagac cagcgggatg cggtgcggat caaagtcgat cccgaacaca cgcagatgtt
     6241 ccgcatcctg tccgttgagc aggctgttcc acgagtaact cgaccgggtt tgctcgtaca
     6301 cccgcctgaa atcggaagga cacttgagga cttgcgacga ccccaaatgg ttggtcagga
     6361 ccacgtcaat tgtcgcctgg ctgtcgccag acgacacacc attggtttcg aacagtcggt
     6421 cgcgcatttc gggaagacgg ttgttgttgt cctgcgtgta gagttgcagc gcgatcccga
     6481 tttggcgcag gttgcccagg caggcggtcg agcgcccggc ctcttgcgcc ttgctcagcg
     6541 tcggcagcag caggctcgcc aagaccccaa taatggcaat caccaccagc aactcgatca
     6601 gggtaaaggc agtcgtctgc aaccgggcgt tcatctcccc ccccgaagaa agacaccgct
     6661 ctccatcatg tgctgcagct cctcgagcaa cggcgccgcc tcggccttgg tttgagccga
     6721 actctccgaa tagaatgact tgaggccggt ggtgatcagc ttctggcgca cctcctcgct
     6781 caacactggg ggcgcattcg tttcccactc gggcatcggc aggtcgcgtg ccgccagctc
     6841 ttggcgcgcc tctttgagcc gcttgaccgc ctcgtccacg gttcggcggc ggcgatcgac
     6901 aggcaactgc tcgaatgccc caagcatctg tttgaatccc gtcggcagcg tggcctcgat
     6961 gatttccgac ttttcagtct ccgtcatcgc gagcaaccac cgcgaccact cctgatcgag
     7021 tcgcgccctc tgacgctcct cgtaggacaa tgcgttcagc ttcgacgcaa gctcgcgcag
     7081 cgccttcgct cgcgcctcgc cggtcagtg
//
LOCUS       DAOQRO010000162         7101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10349_length_7101_cov_4.220409, whole genome shotgun sequence.
ACCESSION   DAOQRO010000162 DAOQRO010000000
VERSION     DAOQRO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10349_length_7101_cov_4.220409"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1386
                     /locus_tag="PKK20_07395"
     CDS             448..1386
                     /locus_tag="PKK20_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99749.1"
                     /translation="MHPVLRIALYVVLAASAVFFGYRFMQGYADRMDRAAHRYESEMT
                     SAPVEAEPASDIALESTNALPPAADIPPETEGDSDTAAASINEVATEGEGATTNAPPP
                     PAAASTPPSAAALRSGGVGIYAALALGAVIGLALLVASDVSHYMGLRAQRALYSDEGE
                     HASSPEYDQAEQAWANGDHMEALRLLRDHLNRHPRQLHAAFRIAEIYEKDLGNHLAAA
                     LEYEEILKHKLAPDRWGWSAIHLCNLYNRIGQFDKTEALLRQIVDEYGETPAAKKARE
                     RLGIPEASPLAGPAESEPQPAAGPQLPPGFRPKKKR"
     gene            1383..3737
                     /locus_tag="PKK20_07400"
     CDS             1383..3737
                     /locus_tag="PKK20_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNU99750.1"
                     /translation="MSQPIAPSSQASHAAAAGPKFQPYILPEVTMREFTPRAVVMGTL
                     LGMVFGASSLYLVLKVGLTVSASIPVAVIAITLFRLLSKFGFKDATILENNIVQSAGS
                     AGESIAFGVGVTMPAILILGFDLELTRVLLVAILGGLLGVLMMIPLRRALIVAQHGIL
                     KYPEGTACAEVLKAGASSESKALSSEAQKAQTIAGLGAQTNPATIFIGFAIGLVYKTL
                     MKLFLLWKEIPERIFGAPYKGASVSAEISPELLGVGYIIGPRIASIMCAGGVLSYLVL
                     IPAIKFFGDHVAGVVPPGIRPIGEMGPHELRGAYVLYIGAGAVAAGGIISVLRSLPII
                     LHALRSGLRDFSGARGGATGGLRTDRDLPLSFILIGILALLAAISLSTPLNMNLLGAL
                     MIVVFGFIFVTVSSRLTGEIGSSSNPISGMTVATLLLTCLIFLMLDWTEARYYVTALS
                     VGGIVCIASSNGGTTSQSIKTGYLVGGTPRAQQLALLLGALASALILGPVLMRLNSTT
                     TVYVPVGSLPNIPATLRADATALTKREALQGPQAVTDTREYLVWHNTDQRLGPEGRYL
                     VDDQGAPVYLVDPGINGAHRTRPDGSTVEKFDAPKAVLMSYIIKGILSRELPWGLVLV
                     GAAIALVLEMSFLPSLAFATGVYLPLSSSSPIFIGGLVRWLVDLYVRKKHVSARLTED
                     QLAAEGDKSAGVLMASGYIAGGAIAGILIAFVAGLADPNETASWFQQKLAAFNSTMLA
                     WSANNPLVTGGWADLLALLPFLVLVGLLYGAGRELILARKQPGR"
     gene            complement(3860..4786)
                     /locus_tag="PKK20_07405"
     CDS             complement(3860..4786)
                     /locus_tag="PKK20_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNU99751.1"
                     /translation="MPARCRAEAVTPCKSFDLAAYLEKSASAVNRALDAFLPKATVKP
                     VTIHRAMRYSLFAGGKRMRPALCLAAAEACGGRRAEAMPLACAVECIHTYSLIHDDLP
                     AMDNDDLRRGKPTNHKVFGEGIAVLAGDALLTQAFEIAAQCRGWPCYSHRDIVLELAR
                     ASGSLQLVGGQVADLEAEGKSISKSQLRYIHERKTSALLCCSVRLGGMSANCTPRQLV
                     ALTDFGYHVGLAFQVIDDILDVTQTSQQLGKTAGKDTAVQKATYPAIVGLDRSRRIAA
                     ELTQLAFEALAPFRGRAQALEAIAQYVLRRDR"
     gene            4785..4964
                     /locus_tag="PKK20_07410"
     CDS             4785..4964
                     /locus_tag="PKK20_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99752.1"
                     /translation="MSLHVGNRKGRRKHRLNGLMPSGECAVVRSVAPVKSHGVLARRF
                     AQVTTRLGSKAYRYL"
     gene            complement(4955..>7101)
                     /locus_tag="PKK20_07415"
     CDS             complement(4955..>7101)
                     /locus_tag="PKK20_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU99753.1"
                     /translation="GVPPGTATRSHLTVDNLACSEDQAPALLRIQADGANGANLAWPG
                     WHIPLESDSVLGPWRPRPARVSLGEGEYRSAVPPADAGRFYRLARGVHVFDSFDGGTT
                     WDMASVLPDGLWHPTWTVLADRSCGRILGAGTQNQDFVLRSGSGIEYGDCVAVVDIIG
                     WGEAMEGAAFGIVLRANSGQDTWQSDTPGLPEERYAGLLTFMQADPPFESALSITGPG
                     GAVLGEQNFPALHPNQQYRLRFWAVGDQLTLELFDRENLDRPIQTCLVTDGRIGEGMA
                     CLYGTRSASGTFDVTVDEFMLNGVTVVSDLPWDFGVAWSAERSLWAHQMIIQDGVVYS
                     NWAGASAGTEGELTAIDLKTGTVLRSIQGVSFQTAPLLVDGRLFSFWAGPVAAYNSVM
                     YELDAETFTILQSHDVTGTVYQENIGYDASLRHAFLRRNIVGKSAISAFNIDTWEAVW
                     TTDPNAPDYFLGDGYFNGTSVLSIQDGRAYAHGRDSDGENFIIALDAKTGERVWKSAS
                     IGFTGGAPGDYAYNNPIYGRDADLIFASTCWASISALRPANGEILWSRNLRGSGYKVT
                     STLTFDHDNRVFVGLHHPSGGAYTCLDAGTGEVLWTSEGYSEVDPQTGLTVYDDGWSA
                     GWVDDVFFYQNTHAGPGAIVVRNKRTGDIVWTYDTENQPCTNPVAAAGLVLFGNGRYL
                     VAVRAGYGEATSCPWHGQDHSGCLPGAVTHYR"
BASE COUNT         1275 a         2326 c         2195 g         1305 t
ORIGIN      
        1 gcttcaacct gtggcgcgcg ctgcgcggcc cggtctcgac cgcctgccca gcatcgattc
       61 tccgccgcca gccccatgca acactgtttc tagatgccga gtcggctgcc gaggtttagc
      121 gagtccctga ctcgagacaa tcagcgtcat cgatcagtgc ctctcccgcc aacgcaggat
      181 cggccctccg aaagggggat tcatgggcag cattgacatt caacagttta atgcgcacgg
      241 ggaccatgaa ccggtgcgga gcggccctgc ctgccggcag gcagggccta gtcgccccaa
      301 actccttcaa aaacgctctt cggcgggggg cgagaccctg cctgccggca ggcaggcgtc
      361 tcccctccaa ggttcgattc acgggcagat gcttaaaact gcttttcctg ggccgctggt
      421 ttctctaggc tccgcccggt ttgctggatg catccggttt tacgcatagc cttgtacgtg
      481 gtcctggccg caagcgcggt cttctttggc tatcgtttca tgcaggggta tgccgatcgc
      541 atggatcgcg cggcccatcg ctacgagagc gagatgacat ccgcccccgt cgaggccgag
      601 cccgccagcg acatcgccct cgagtccacg aacgcgctgc cgcccgccgc ggatattcct
      661 ccggaaacgg aaggcgattc cgacacagcc gcggcatcga tcaacgaagt cgcgacggag
      721 ggggaaggcg caaccacgaa cgccccgccg ccgcccgccg cggcgtcaac tcccccctca
      781 gccgcggcgc tgcgatcggg gggggttgga atctacgccg cgctggcgct gggggcggtc
      841 atcggcttgg cgttgctggt ggcaagtgat gtgtcgcatt acatggggtt gcgggcgcaa
      901 cgcgccctgt acagcgacga aggcgagcac gcgagcagtc ccgaatacga ccaggccgag
      961 caggcctggg cgaatgggga tcacatggag gcgctgcgcc tgttgcggga tcatctcaac
     1021 cggcaccccc gtcaactgca tgcggccttt cggattgcag agatctacga gaaggatttg
     1081 ggcaatcacc tggccgcggc cctcgaatac gaggagattc tcaagcacaa gctcgctccc
     1141 gaccggtggg gatggtccgc cattcacctc tgcaacctct acaatcgcat cggtcaattc
     1201 gacaagacgg aggccttgct gcggcagatt gtcgacgagt atggcgagac gccagccgcc
     1261 aagaaagccc gcgaacggct cggcattccc gaagcaagcc ccttggctgg accggccgaa
     1321 agcgaaccgc aacccgccgc cggcccgcag ttgccgccgg gtttccgacc caaaaagaag
     1381 cgatgagcca gccgattgcc ccttcctcac aggcgtcgca cgccgcggcc gccggtccga
     1441 agttccagcc ctacattctg ccggaggtga cgatgcgcga gttcacaccg cgcgcggtgg
     1501 tcatgggcac cctgctgggc atggtgttcg gcgcgtcctc gctctatctc gtccttaagg
     1561 tcgggctgac ggtcagcgca tcgattcccg tggcggtgat tgccattacg ctgtttcgct
     1621 tgctctccaa gttcggattc aaggatgcga ccatcctgga gaacaacatc gttcagagcg
     1681 ccggatccgc cggcgagagt atcgcgttcg gcgtgggagt caccatgccc gccatcctga
     1741 ttctgggctt cgacctcgag ctcacgcgcg tcttgctggt ggcgattctg ggcgggcttc
     1801 tgggcgtgct gatgatgatc ccgctccgcc gggcgctcat tgtggctcag cacgggatac
     1861 tgaagtaccc ggaaggcacg gcctgcgccg aggtgctcaa ggccggcgcc tcgtcggaat
     1921 ccaaggcgct ctcctccgag gcgcagaagg cccaaaccat cgcgggtctg ggtgcgcaga
     1981 ccaacccggc caccatcttc atcggattcg ccatcgggct tgtttacaag accctcatga
     2041 agctcttcct cctgtggaag gaaatcccgg agagaatctt cggcgcgccg tacaagggcg
     2101 cgtcggtctc ggccgaaatc tcacccgaac tgctcggcgt cgggtacatc atcggcccgc
     2161 gcattgcctc gatcatgtgc gcggggggcg tgctctcgta cctggtcttg atcccggcga
     2221 tcaagttctt cggcgatcat gtcgcagggg tggtgccgcc gggaatccgg ccgatcggcg
     2281 aaatgggccc ccacgaactg cgcggggcct atgtgctcta catcggagcc ggcgccgtgg
     2341 cggcgggcgg catcatcagc gtgttgcgtt ccctcccgat cattctccac gccttgcgca
     2401 gcggtttgcg cgacttcagc ggggcgcgcg gcggcgcgac aggcggtctg cgcaccgatc
     2461 gcgatctgcc tttgagtttc atcctcatcg gcatcctggc tttgctggcg gccatctcgc
     2521 tctcgacccc gctgaacatg aatctcctcg gagcgctcat gatcgtggtg ttcggcttca
     2581 tcttcgtcac ggtctcctcg aggctcaccg gcgagattgg ttcgtcctcg aatcccatct
     2641 ccggcatgac ggtcgccacg ctgctgttga cctgcttgat cttcctgatg ctcgattgga
     2701 ccgaggcgcg gtactacgtc accgcgctgt cggtcggcgg cattgtctgc atcgcctcct
     2761 cgaacggcgg cacgacttcc cagagcatca agacgggtta cctcgtcgga ggcacgccgc
     2821 gcgcgcaaca gctcgcgctg ctgctcgggg ccctcgcctc cgctctgatc ctgggtccgg
     2881 tcctgatgcg gctcaactcg accacaacgg tttacgtccc ggtaggctcc ttgcccaaca
     2941 tcccggccac actgcgagcc gacgcgacgg cgctcacgaa gcgggaagcg ctccaagggc
     3001 cgcaagccgt caccgacacc cgggaatacc tggtctggca taacaccgat cagcggctcg
     3061 gcccggaggg gcgttacctg gtggacgacc agggagcgcc ggtctacctg gtcgacccgg
     3121 gcatcaacgg cgctcaccgg acccgtccgg acggctcgac agtcgagaag ttcgacgcgc
     3181 ccaaggcggt gctgatgagc tacatcatca aaggcatcct cagccgcgaa ctgccctggg
     3241 gactggtgct ggtcggcgcc gcgattgcgc tggtcctcga gatgtcgttc ctgccgtccc
     3301 tcgcattcgc caccggggtc tatctcccgt tgtcctcgtc gtccccgatc ttcatcggcg
     3361 gactggtccg ctggcttgtg gacctgtacg ttcgaaagaa gcacgtcagc gccagactga
     3421 ccgaggacca attggccgct gagggtgaca agagcgccgg ggtattgatg gcctcgggct
     3481 acatcgccgg aggcgcgatt gcgggtattc tgatcgcttt cgttgccggg ctcgccgatc
     3541 caaacgagac cgcctcctgg ttccagcaga aactcgcggc attcaactcg acgatgctcg
     3601 cctggtcggc caacaacccg ctcgtcacgg gcgggtgggc cgatctcctg gccctcctgc
     3661 ccttcctggt cctggtcggc ctcctctacg gcgcgggacg cgaactcatc ctggcgcgca
     3721 aacaaccggg acgttagctt cattggaagt caaacgatca ctcaccaagc ggctgagaag
     3781 accatggggc attgtaccgc gccccctccc gtgcaccggg cgatcagact ctgggcgatg
     3841 actgcaagac ccgtcaggct caccggtcgc gcctgagcac atactgggcg atggcctcca
     3901 gagcctgggc gcggccgcgg aaaggggcga gggcctcgaa cgcgagctgg gtgagttcag
     3961 cagcgattcg ccgcgatctg tcaaggccga caatggccgg gtaggtcgcc ttttgcacgg
     4021 ctgtgtcttt gccggcggtc ttgcccagtt gctgactggt ctgggtgaca tcgagaatgt
     4081 cgtcgatcac ctggaaggcg agtccaacgt ggtagccgaa atcggtgaga gcgacgagct
     4141 gtcggggcgt gcagttggcg ctcatccccc cgagccggac cgagcaacag agcagggccg
     4201 aggtcttgcg ctcgtgaatg taacgcaatt gggatttgga gatcgacttg ccctcggcct
     4261 cgaggtcggc cacctgaccg cccaccagtt gtagcgatcc cgaggctcga gccaactcga
     4321 gaacgatgtc ccggtgggaa tagcacggcc aaccccgaca ctgggccgcg atctcgaacg
     4381 cctgggtcaa cagcgcgtcg ccggccagca ccgcaatgcc ctctccgaag accttgtggt
     4441 tggtcggctt gccccggcgc agatcgtcgt tgtccatggc cggcaggtcg tcgtggatca
     4501 gggagtaggt gtggatgcac tcgacggcgc aggccagagg catggcctcg gctcggcgtc
     4561 cgccgcaggc ttcggcggct gccaggcaga gcgccggtcg catgcgtttg ccgccggcga
     4621 acagggaata gcgcatggcc cgatggatgg tgacgggctt gacggttgcc ttggggagaa
     4681 aggcatcgag agcgcggttc acggccgagg cgctcttctc gagataggcg gccaggtcaa
     4741 acgatttgca gggcgtcact gcctctgcgc gacaacgtgc aggcatgtcc ctgcatgtag
     4801 gcaatcgcaa aggccgacgc aagcacagac tcaacgggct gatgccctcg ggcgagtgtg
     4861 cggtcgtgcg atcggtcgct cctgtgaaat cccacggggt gctggcacgg cgattcgccc
     4921 aggtgacaac tcggttgggg agcaaggcgt accgctatct ataatgcgtg accgcgcccg
     4981 gcaggcatcc tgaatgatcc tgcccatgcc acgggcagga cgtggcttcg ccatatccag
     5041 cccggactgc aaccagataa cgcccgttcc cgaacaggac aaggccagcg gcagcgactg
     5101 gattcgtgca gggttggttc tcggtgtcgt aggtccagac gatgtcaccg gtgcgcttgt
     5161 ttcgcacgac aatcgcgccc gggccggcat gggtgttctg atagaaaaaa acgtcgtcca
     5221 cccaacccgc actccaacca tcgtcataca cggtcaggcc agtctgcggg tcaacctcag
     5281 aatagccctc actcgtccac aggacctcgc ccgtgcccgc gtccaggcat gtgtacgccc
     5341 ctccggatgg atgatgcagt ccgacaaaca cacgattgtc atggtcgaag gtcaatgtgg
     5401 atgtgacttt gtagccggat cccctcaggt tgcggctcca gaggatttca ccgttcgcgg
     5461 gcctcagagc ggagatgctc gcccaacagg tgctggcaaa aatgaggtcc gcgtctcgtc
     5521 cgtagatcgg gttgttgtag gcgtagtctc cgggtgcccc ccccgtgaaa ccgatggaag
     5581 ccgacttcca gactcgttcc ccggtcttgg catcgagcgc aatgatgaag ttctctccat
     5641 cgctgtcgcg tccgtgggcg taggcgcggc catcttggat ggacagaacg gacgtgccat
     5701 tgaaatagcc atcgcccaga aagtaatcgg gagcgttcgg atcggttgtc cagacggctt
     5761 cccatgtgtc gatattgaag gcactgatgg cgcttttacc aacgatgttc cgtcgcaaga
     5821 aagcatggcg aagcgaggcg tcgtaaccga tgttctcctg gtagaccgtg cccgtgacgt
     5881 cgtgcgattg cagaatcgtg aaggtctccg cgtcgagttc gtacatgacg ctgttgtagg
     5941 ccgccacggg accagcccag aacgagaaaa gcctgccatc gaccagcagg ggcgccgtct
     6001 gaaatgaaac accttgaatc gacctcagca ccgttcccgt tttcaaatcg atcgcggtca
     6061 gttccccctc agtaccggcg ctggcgcctg cccagttgga gtacaccaca ccgtcctgga
     6121 tgatcatctg gtgcgcccag aggctgcgtt ccgcgctcca cgccacaccg aagtcccacg
     6181 gcagatcgga cacaacggtc accccgttca gcatgaactc gtcaacggtg acatcaaatg
     6241 tcccgcttgc cgacctcgtc ccgtagaggc acgccatccc ctcgccgatc cgcccatccg
     6301 tgaccagaca cgtctggatg ggcctatcga ggttctcccg atcgaacaac tcaagcgtca
     6361 attggtctcc cacggcccaa aaccgcagcc ggtattgttg gttggggtgc agcgcaggga
     6421 agttttgctc ccccagcacc gcgccgccag gccctgtgat ggacagcgcg gactcgaacg
     6481 gggggtccgc ctgcatgaaa gtcagcaaac cggcgtaacg ctcctcggga agcccggggg
     6541 tgtcactctg ccacgtgtct tgcccagagt tcgcccgcaa cacgatccca aacgccgcgc
     6601 cttccatggc ttcgccccag ccgatgatgt ccaccacggc gacgcagtcg ccatactcga
     6661 tgccagaacc cgatcgaagc acgaagtcct ggttctgtgt gcctgccccc aaaatgcgtc
     6721 cacaactccg atcggcaagc acggtccagg ttgggtgcca caggccatcc ggcagaacgg
     6781 atgccatgtc ccaggtcgtc ccgccatcga acgagtcgaa aacatggacg ccccgagcca
     6841 gtcggtaaaa ccggccggca tcggcgggcg gaacagccga cctgtattcc ccctccccca
     6901 acgacacccg cgccggccga ggcctccacg gcccaagaac ggaatccgat tccagcggga
     6961 tgtgccaccc tggccatgcc agattggccc cgttcgctcc gtcggcttga attctgagaa
     7021 gagctggggc ctgatcctcc gagcaggcca ggttgtccac ggtcaagtgc gagcgcgtcg
     7081 cggtccctgg aggaactccg a
//
LOCUS       DAOQRO010000163         7056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10445_length_7056_cov_5.542637, whole genome shotgun sequence.
ACCESSION   DAOQRO010000163 DAOQRO010000000
VERSION     DAOQRO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10445_length_7056_cov_5.542637"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..660
                     /locus_tag="PKK20_07420"
     CDS             139..660
                     /locus_tag="PKK20_07420"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017115925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNU99754.1"
                     /translation="MSRNIPKGTVVIAIVGATIGYTGVLAYDMCVTDSMVGIETGTPE
                     GNRYVEFFLRHKKHEIRQASYSSGGQPNIKLEFLNPYPLPVPPLPEQREIVRRVEALF
                     ALADRIEARFQKALAQVDKLTPSILAKAFRGELVPQDPNDEPADALLQRIRFGMYARM
                     LACPMPRTTTGGS"
     gene            772..1194
                     /locus_tag="PKK20_07425"
     CDS             772..1194
                     /locus_tag="PKK20_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNU99755.1"
                     /translation="MLKQTVTEFTVATRGRGLHEVTHDVAAWVSHSGIRTGLITLHVR
                     HTSASLLIQENADPDVQEDLDRFFARLAPDGDPIYRHTIEGEDDMPAHVRAALTAVNL
                     SIPIIGGALALGTWQGIYLWEHRKHRHTRRIAAHLIGE"
     gene            1590..3263
                     /locus_tag="PKK20_07430"
     CDS             1590..3263
                     /locus_tag="PKK20_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNU99756.1"
                     /translation="MHSPKTSIVTNALSRGVGSPEVDSQPARAFEAGMFDPRASTAAF
                     PGCGCCPLSRRSFLKKGGAACVTALAMSARPVEATEGPAGRPRIRIVYVLFGPKQPRP
                     TWPNIGFDFVPVMQRLEARLIRECPGFEFVTSMASTEAEAKKILEEDQNSSVIGYLVY
                     QMNNWPRVVQAIAASGKPVLYADFTYAGTGGFLTYNAALLRARSPNVGFVSSSELADL
                     TEAVKCFELVRQGRTPSDFVAATAQLRQRRTPPPESAAWAEDPVQCLSIPECVSRMKA
                     SKVIAIRDQNSAPATEIMGIPIVYVPFAELNGLAQAADPDEVRLWADRWEHGARRIEG
                     VTRETIERSAAMYLGQKALLQKHSANAITINCLGGFYGKHINAYPCLGFCQLVDDGLV
                     GACECDIRSTATMVAITTLTQGRPGYISDPVMDSAKREIIYAHCVAPTRVFGPSGAAN
                     PYEILTRSEDRQGASLRSLMPVGYLTTTLEFEPKRKEIMFHQAKAVGNDANARACRTK
                     LRAQPLGDFEKLFSMWDVWGWHRVTFYGDLKEPVYALADSVGWKVVTET"
     gene            3260..5095
                     /locus_tag="PKK20_07435"
     CDS             3260..5095
                     /locus_tag="PKK20_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNU99757.1"
                     /translation="MSELSHLKHLAPGLTAAFLFVLAPCRPLLAQNADSPGPIHLRVN
                     LLGYRPSDPKIAIAFSTQPLAQNFEVLTTADQTVVWRGPATPMRDTVWGRWHHHAELD
                     FSAFRQAGRFVVRLGPTNSPAFEIGTQARDDACGALLEFMRQQRCGDNPWLDAQCHQQ
                     DGRTAYGPRPAGTPIDARGGWHDAGDLLKYLMTSANATADMLLAYRIAPNAFPDLVRQ
                     DGRPGTNGVPDVLDEARWGLDWMLRLHPTPQELYHQVADDRDHWGWRLPADEKADYGW
                     GPGGPRVVYAADGRPQGLGKHQSESTGLGNLAGRYAAAMAMAYQVWRDSAEHGVFAQR
                     CLQAGLEVYALGRAREGFQQGNSYGAPYRYNESTWADDMEWGAAELYRATGESRYLED
                     ARRYAREAGAESWMGRTEARHYEFYPFVNIGHFAFHDIADEETGKMLIGYYRDGIERC
                     VTAGRRTPYRIGVPFIWCSNNLTVALATQCLFYERMSGDSRYREFAAAQSDWLLGRNP
                     WNYSMFTGLPPGASSPRNPHLMTTRLTGRMVRGGLVDGPVYEQVFRSLKGVSITQPDP
                     LAEFQSDLAVYHDDLHDYSTNEPTMDGTASAILLFALLAESPHFR"
     gene            complement(5160..5837)
                     /locus_tag="PKK20_07440"
     CDS             complement(5160..5837)
                     /locus_tag="PKK20_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNU99758.1"
                     /translation="MVRSVLFDIDGTLIRTGGASVRAFERTGQSVFHVADGVRHMRFA
                     GRTDRSLIREFFRRHGIEATPANFDRFLDHYVFVLDQLLGELHGQVCPGVGDFIRDLR
                     DLAQPPLLGLLTGNIRLAAEIKLRHFGLWDYFTTGAFGDDHEDRNELAGIARERVSSV
                     LRRPLGGDEIVVVGDTPLDIECGRAIGAKVLAVGTGGAELGVLRAHRADWVVETLADM
                     RAATVCR"
     gene            complement(6279..>7056)
                     /locus_tag="PKK20_07445"
     CDS             complement(6279..>7056)
                     /locus_tag="PKK20_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNU99759.1"
                     /translation="HGINLSLGRAAEISLVAHFFNSFLLGSTGGDLMKAFYAARETSN
                     RKTEAVVTVLVDRLIGLLSMLLFAAVMMVPNWALLSGHRRLAALAGIVVIMLMGALAV
                     AGLSFWGGLSRRWPRARIWLRRLPKGELLERALEACRQFGQQPRFLVQAFALSTVLNV
                     MCVLQIWVLARGLDLTIAPVALSLIVPVIICIAAMPVTPSGLGLRENIYVWMLAVPEI
                     NIPATQALLLSLLAYAGSLFWSLVGGVVYLFFRATRTQQT"
BASE COUNT         1352 a         2216 c         2259 g         1229 t
ORIGIN      
        1 ggttttcgtc aagcttccaa ccattttttt cgccaattcc tgtgggatgg ctgtgcattc
       61 attcagattg ggaaccttcc tcgcgaaggt ggcttggtca cgtcgcacac ccagactttg
      121 aacgacgaag gacttgttgt gagccggaat atccccaagg gaacggtggt gatcgcaatt
      181 gtgggggcga ctatcggcta cacgggcgtt ctggcttacg acatgtgcgt cactgacagc
      241 atggtcggca tcgagacagg gacccccgag gggaatcgct acgtcgagtt cttccttcgt
      301 cacaagaagc acgagattcg acaggcgtcg tactcgagcg gcggtcaacc caacatcaag
      361 ttggagttct tgaaccccta cccacttccc gtcccgcccc tccccgaaca acgagaaatc
      421 gtgcggcggg tggaggcgtt gtttgctttg gcggaccgga tcgaggcacg gtttcagaag
      481 gccctggccc aggtggacaa gctcacgccc tcgatcctcg ccaaagcctt ccgcggtgaa
      541 ctcgtgccgc aagacccgaa cgacgagccg gccgatgcac tattgcagcg cattagattt
      601 ggaatgtatg cacgcatgct ggcatgtcca atgccgcgga cgacgacagg tggttcttga
      661 actgcgcccg ccgggggatg aaggtcaaag cggtgattga tcatcgacag actgcaagac
      721 ggatggcacg aacccgagac accggcacgc tgtgaaagcc ggtcgaacac catgctgaag
      781 cagaccgtca ctgaattcac cgtggccacg aggggacgcg gcctccacga ggtgacacac
      841 gacgtcgccg cgtgggtgtc gcacagcggg atccgaaccg ggttgatcac gctccatgtg
      901 cgccacacct cggcgtcgct gctgatccag gagaacgccg atccggacgt gcaagaggat
      961 ctggaccggt tcttcgcgcg tctggcgcca gacggcgatc ccatctaccg acacacgatc
     1021 gagggcgagg acgacatgcc ggcgcatgtg cgcgcggcgc tgacagccgt gaacctgagc
     1081 atcccgatca tcggcggcgc gttggcgctg ggcacgtggc agggcatcta tctctgggaa
     1141 caccgcaaac accgccacac gcgtcggatc gcggcccatc tgatcggcga gtgaagagcg
     1201 ccggctttcg tgatgccgtg tcctctgcgc gctctgcgtt tctgtggttc aatccgggac
     1261 ggtgactgtc atcggatggg attcaccaca gagacacaga gtacacggag gggaaagtgc
     1321 ctcataaact cggggccgat catgaaagac ggatgacttc ccgcgtttcc atccatgtcc
     1381 tgccccgctt catgagggat ttggggttgg acccgcaggc gaacacccgc gcgtgaaaac
     1441 cgcgggcgaa aaactgttgc gccggtccct ggccacagcc agtattcgca agctctaacc
     1501 cgcatatgac gcacacccca tcagtgaaca gggtggtcag acctcgcaag atgtcgggta
     1561 tgcgggttaa acaacatcca gtctgtccta tgcatagtcc caaaacctcg atcgtcacga
     1621 acgcgctgtc ccgtggcgtt ggctcacctg aggttgattc ccagccggcc cgggctttcg
     1681 aagcgggcat gttcgatcct cgggcttcga ctgcggcgtt cccgggctgc gggtgttgtc
     1741 ccttgagccg gcgctcgttt ctcaagaagg gcggagcggc ctgtgtaacc gccctggcca
     1801 tgagcgcccg accggtcgag gccacggagg gcccggcggg tcggccgcgc attcggattg
     1861 tctatgtcct gttcggcccc aagcagccgc gcccgacctg gccgaacatc ggattcgatt
     1921 tcgtgccggt catgcagcgg ctcgaagcga ggctgatccg ggaatgcccg ggattcgagt
     1981 tcgtgacctc gatggccagc acggaggcgg aggcgaagaa gatactcgag gaggaccaga
     2041 actcgagcgt gatcggctac ctagtctatc agatgaacaa ctggccccgg gtggtgcagg
     2101 ccatcgccgc ctcgggcaag ccggtgttat acgccgattt cacctatgcg ggcacaggcg
     2161 ggttcctgac ctacaacgcc gcgctgctgc gggctcgatc accgaatgtt ggcttcgtct
     2221 cgtcgtcgga gttggccgac ctgaccgagg cggtgaaatg cttcgaactc gtccgccaag
     2281 gtcggacacc gtctgatttt gtggcggcga ccgcgcagtt gcggcaaagg cgcaccccgc
     2341 cgccggagtc cgcggcctgg gctgaggacc cggtgcaatg cctgtcgatt cccgagtgcg
     2401 tgagccggat gaaggcatcg aaggttattg ccattcggga ccagaactct gcgcctgcga
     2461 ccgagatcat gggcatcccg atcgtatatg tgccgttcgc cgagttaaac gggctcgccc
     2521 aggcggccga tcccgacgag gtgcggcttt gggccgatcg ctgggagcac ggggcgcggc
     2581 ggatcgaagg ggtcacgcgc gaaacgatcg agcgatcggc ggcgatgtac ctgggccaga
     2641 aggcccttct gcagaaacac agcgccaacg ccatcaccat caattgcctc ggcggcttct
     2701 acggcaaaca catcaacgcc tatccctgcc tgggcttctg ccaattggtc gatgacggcc
     2761 tggtgggcgc ctgcgaatgc gatatcaggt ccacggcgac catggtcgca atcaccaccc
     2821 tgacccaggg ccggcccggt tacatttcag acccggtcat ggattcggca aaacgcgaga
     2881 tcatctacgc tcactgcgtg gcgccgacca gagtcttcgg cccgtcgggc gcggcgaatc
     2941 cgtatgaaat cctcacccgt tccgaggacc gccaaggcgc ctcgttgcgc tcgctgatgc
     3001 cggttggcta tctgacgacg acgctcgaat tcgagcccaa acgcaaagag atcatgtttc
     3061 accaggccaa ggcggtaggc aacgatgcca atgctcgcgc ctgccgcacc aagctccgcg
     3121 cccagcccct gggcgatttc gagaagctct tctcgatgtg ggatgtctgg ggatggcacc
     3181 gcgtcacatt ctatggcgac ctcaaggaac cggtctatgc cttggccgat tccgtgggtt
     3241 ggaaggtggt gactgaaacg tgagtgagct aagccacctg aaacacctcg caccggggct
     3301 gacagccgcc ttccttttcg tcctcgcacc gtgccggcct ttgctggcgc aaaacgcaga
     3361 cagcccgggt ccgatccacc tgcgcgtgaa cctgctcggg tatcgcccat ccgatccgaa
     3421 gatcgccatc gctttttcca cgcagccttt ggcacagaac ttcgaggtgc tgacgaccgc
     3481 ggatcaaacc gtggtctggc gcgggccggc gacaccaatg cgggataccg tctgggggcg
     3541 atggcaccac catgccgaac tcgatttcag cgcctttcgc caggcaggca gattcgtcgt
     3601 ccgcctcggc ccgacaaact cgcccgcatt cgagatcggg acgcaggcgc gggacgatgc
     3661 gtgcggggcg ttgctcgaat tcatgcgcca gcagcgctgc ggcgacaatc cgtggctcga
     3721 tgcccaatgc catcaacagg acggacgcac ggcctacggg ccgcgtccgg ccggaacacc
     3781 catcgatgcg cggggcggtt ggcacgatgc cggcgacctg ctcaagtacc tgatgacctc
     3841 cgccaacgcc accgccgaca tgcttttggc ctatcggatc gctccgaacg cttttccgga
     3901 tctggtccgg caagatggac gccccggaac gaacggcgtc cccgatgtcc tggacgaggc
     3961 gcgatggggt ctcgattgga tgttgcggct ccatccaact ccgcaagaac tctaccatca
     4021 ggtggctgac gaccgggacc actggggatg gcggctgccc gcggacgaga aggccgacta
     4081 cggatgggga cccggcggac cgcgggttgt ctacgcagcc gacggacgtc cgcaggggct
     4141 cggaaagcac caaagcgagt caactggcct gggcaatctc gccgggcgct acgctgcggc
     4201 catggcgatg gcctaccagg tctggcgcga cagcgccgag cacggcgtct tcgcccagcg
     4261 ttgcctgcag gccgggctcg aagtttacgc cctgggacgc gcgcgcgagg gttttcagca
     4321 gggcaactcc tacggcgcgc cttatcggta caacgaatcg acctgggccg acgacatgga
     4381 atggggcgct gccgagctct accgggcgac cggcgagagc cggtatctcg aggacgcccg
     4441 ccgctatgcc cgagaggcgg gggcggaatc ctggatgggg cggaccgagg cgcggcacta
     4501 cgagttctat ccgttcgtga acatcgggca tttcgcgttc cacgacatcg cggatgagga
     4561 gaccgggaag atgctgatcg ggtactacag ggacgggatc gagcggtgcg tcactgccgg
     4621 gcggcgcacg ccgtatcgga tcggcgtgcc gttcatatgg tgctcgaaca acctgacagt
     4681 ggcgttggcg acccagtgcc tcttttacga acgaatgtcc ggcgactcgc gttaccggga
     4741 gttcgccgcg gcgcagtcgg attggctgct gggacgcaat ccgtggaact actcgatgtt
     4801 caccggcctt ccgcccgggg cctcctcacc cagaaatcca catctgatga ccacccggct
     4861 caccggacgg atggtgcggg gcggtctggt ggacggtccg gtctacgagc aggtgttccg
     4921 cagtctcaaa ggcgtcagca tcacacagcc cgatccgctg gcggagttcc aatccgattt
     4981 ggcggtgtat cacgacgatc tccatgatta ctcgaccaac gagcccacca tggacggcac
     5041 ggcgtcggcg attctgctct tcgcgctcct ggccgagagt ccgcatttca ggtgaactgt
     5101 gacggtgacc ggtcaggtga cttgagcagt tggcgcgaaa tgcgggctgg cgcaggcctt
     5161 caccgacaga ccgttgccgc cctcatgtct gccaacgtct ccaccaccca atccgcccgg
     5221 tgcgcccgca gcacgcccag ctcagccccg cctgtgccga ccgccagcac cttggctccg
     5281 atcgcccgcc cgcactcgat gtccagcggc gtgtcgccca ccacgacaat ctcgtcgccg
     5341 cccagcgggc gtctcaggac actactgacg cgttcgcgcg cgatgccggc cagctcgttg
     5401 cggtcctcgt ggtcgtctcc gaacgcgccg gtggtgaagt aatcccacag gccgaaatgc
     5461 cgcagcttga tttcggcggc caggcggatg ttcccggtca atagaccgag cagcggcggc
     5521 tgcgccaggt cgcgaagatc gcggatgaaa tcgccaaccc ccggacaaac ctgtccgtgc
     5581 agttcgccga gcaactgatc caaaacgaac acgtaatgat cgagaaaccg atcgaagttg
     5641 gccggcgtgg cctcgatccc gtggcgacgg aagaactcgc ggatcaggga tcggtcggtg
     5701 cgtccggcaa agcgcatatg acgcacgccg tcggcaacgt gaaagaccga ctggccagtg
     5761 cgttcgaagg cgcgaacgct cgcccctcca gtgcggatga gcgtgccgtc gatgtcgaag
     5821 agaaccgagc gaaccacggg ccaagattag cggccgggtg ccgccttgac aatggcgggc
     5881 agcggttgcc gctcgacttc atgaactggc cctgctggcg ggcggtggca gcaacggcgc
     5941 ctcatcgaga gcgttgacat tcatcggttg aatgcgcacg aggaccgtga accgatttgt
     6001 agccgtccct cgccgcgcag acgcgtcaaa ctaatgagaa ttcccgcgct gttcctggga
     6061 gcagcgccgt ggtggtcgtt ctagtctctg gaccggagtt tgttgtggca gtcgcccatg
     6121 ctcgggtagg cgtcgagatc gcagagttcc gcattgcgtc gtcgaggtaa ctggagggtg
     6181 ggcatcctcg gccgcagcaa tgaccgaatg tcctaatttt tgcacagacc ctaaggttga
     6241 cgcgcatgcg cggccgcggg gcggctacgg ttcatgcctc aggtctgttg cgtccgcgtc
     6301 gcgcgaaaga agaggtagac aacgccgcca accaggctcc agaagaggct gccggcatag
     6361 gcgaggagcg agagcaggag cgcctgtgtc gccgggatgt tgatctccgg caccgccagc
     6421 atccagacgt agatgttttc gcgcagcccg agtccgctcg gcgtgacggg catggcggcg
     6481 atacagatga tgaccggcac aatcagggaa agcgccacgg gcgcaatcgt caggtccagg
     6541 cctcgcgcca gcacccagat ctgcaggacg cacatcacgt tcagcaccgt cgagagcgca
     6601 aaggcctgga cgaggaatcg gggctgctgc ccgaactggc ggcaggcttc gagcgcacgt
     6661 tcgagcagtt cgcctttggg caggcggcga agccagatgc gggcccgggg ccagcggcgc
     6721 gagaggccgc cccagaacga tagaccggca accgcgagcg cgcccatcag catgatcacc
     6781 acaatgccgg cgagtgccgc caggcgtcgg tggcccgaca gcagggccca gttcggaacc
     6841 atcatcaccg cggcaaacag aagcatggac agaaggccga tcaaccggtc gaccaggaca
     6901 gtgaccaccg cttcggtctt gcggttgctg gtctctcgcg cggcgtaaaa ggctttcatg
     6961 agatcgcctc cggtcgaccc gagcaaaaac gagttgaaga aatgagccac gagcgagatt
     7021 tccgcggccc gcccgaggga gaggttgatg ccgtgt
//
LOCUS       DAOQRO010000164         7035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10493_length_7035_cov_4.648854, whole genome shotgun sequence.
ACCESSION   DAOQRO010000164 DAOQRO010000000
VERSION     DAOQRO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10493_length_7035_cov_4.648854"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1504
                     /locus_tag="PKK20_07450"
     CDS             263..1504
                     /locus_tag="PKK20_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNU99760.1"
                     /translation="MATVLSSTSTSLFATGFDLPDQDAFAAGRGMAFVATADNPSAIY
                     YNPAGISQLDGHHLRGGVYGLHLGVEYDSPAGRSFDNENPWHAVPQVFYTYGPQKLPL
                     TFGLGVFSPFGLSMEWPQDTGFRTVATEGSLTYMTINPVVAWAIRPNLSVAAGLTVNY
                     AEIDLRQGLFWPSQSYDAFRVKGDGWALGYNLGVLWKPCEKLSFGASFRSITTPNLEG
                     RTSFRNGVTLPLPGGAAVPSFAQQKVDAEADFPFPLKTIVGVSYRPNPRWNFEFNADY
                     TDWNRLNTVTIKQADAFPPLLPQDIPLTFDWESSWYFEWGATRYFDNGWHVSGGYIFN
                     QNSVPDAHYTPLISDLDRHFFSVGAGRKGNHIGFDLAYQFGYGPTRTVRGSAPSASGQ
                     TADGDYTFLSHAVMLTLEYQF"
     gene            1744..>7035
                     /locus_tag="PKK20_07455"
     CDS             1744..>7035
                     /locus_tag="PKK20_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNU99761.1"
                     /translation="MKTPSFRFLCFWSLLWSLLAGGAAAPEGQAQILLNVDFGPGSAS
                     GKVGFAAAGQQTNDFWNAYGHYQPRYQPGTAPIPDGRLEGLRYADGSASSVAVAVTNA
                     PGVWGNATGDPMLDSYMFAPNGSNMVVTLTGLDAGRYHFYLYGWAAPDVAGEQASSFI
                     LRSGTNTLGPLRAPAQEDAPLLHPGRVRMPAVVQRDFPVETDQPVAIEVVPAPGGVAV
                     LNGLQVLSRGTGPPRLAMPDAGPSAVGLTNLLVHSIAYTGEVAAASARFQVSIHATSP
                     TTNHVMAPLFEGELALLDAQLPDGWQIARQGHQFTLHTDTPGSNRIDLTLVARVTREE
                     PWNVVRFTGPPAAAATLSVRSADPHVDLQLVSGVLLEGSDTTQSDVRGVVGADRQVAI
                     RWQAKAAETAREALVTVDTRCSVDATPAAIRLSTSFACEVLQGRCSRIRIALPPGQTL
                     TKLSGDLIKDWQAASEAGLSIITIDFVRPLEGVTAFDVVSEQPLPGLPDAAELRPPQP
                     LGIQRETGRLGVRIEDLVARVDRSEALWQVNAAAGEFAAFQFNLRPAVLHLHLSPVEP
                     LVTASTRARATVEETRLLVDHRVDLNVSRAGIYGLEWSLPPDLTVARVEGEGIEDWQS
                     SAGKLRLRFAQRLLGQRALTIRLEQALNPLPSEIILTPLRLTNTARETALIGAGASAG
                     IQLKTGPLSGAREIPVSGLPERRDELLAFRADAGDWRVNLAVERLSARIVAEVFNLIT
                     IGDGLVGGGATIRFGIVNQGVQQFRVRIPAHWRNVEFTGANIRRKDRDGDIWTITLQD
                     KVWRGYTLVVTYDHGFDPQKATLDAGGAHPLDVERETGAVAITSAANLALEPAPIAEP
                     LRRVDPSELAATDRALVARPVLLAYRYEGPAYALELHLTRHEEVPVLDAVADRAQFTS
                     VLTDSGEMLTQASFLVKNNERAHQRFELAPGATLWGVTVNGEPVRADRDGDWVVVALP
                     RGENRDQIYAIDLKYAQQVGALGGLWPRRLELSAPKTDVPGTYAEWVLHVPTTRHVYD
                     FGGTLEVTGGTTYGWRDGWDQFIATYRGLWHEYGAVLIFGTGLLAFVIALVWYGNRKG
                     FGGVATVLVVFCFLAILAGMLLPALSKAKSKAQRINSLNNLKQIGLAARLYAGDNNER
                     MPDSFEAMRNELGTDKVLIDPETGQRYTYVGAGKSEADPNAIIAYSPERPGGRREVVF
                     ADGSVQIMSADKFLEATAQHQTVPMDQRVFERNGLAGRPQVAPVTPPAQTGIAIPQPT
                     PPQQVPVAPAIQAPTQAPADAPEAMPVFDATGPTAAGIKSIRFDLPKAGRAYTFTRVL
                     SVEEKAPRISVGLVSSRLFTFVRAAVQVLAFLSGLMLVLVQWRSAHPKALWLSVGLML
                     AGLATADLLIAWRVLHLALTLAVPVLLALLPAWLLLRWRQHAKSNRPTPASPPDASPG
                     TDIAGTTSVAGSAVLALVLLNGGPEALAASSLQPLSSRVTITRLDLTGSASPRAAQFD
                     ATLLLAANGTNAFAQLFGSDVAVQESTVMTGEAQFWRNGNAWSVFMPHPGSATLRLKL
                     LAPLGGDLAKPELDLALPPALGGQMALVLSEPDADVEFPDAIGLVRRLEGETTVIEAV
                     LGSNDRLNLRWTPKRKRADEVEATVFADQTALITLGNGAIHTRTVIDWQASQGELRQV
                     CVRLPSGCRLLKVQGPHVRSWQLAPTNADTLVVDLLKPVAPGLRLTLELEKTLDALPV
                     SARIAVPEALDVKRQTGSIGVRAAAELGLGVERARGLD"
BASE COUNT         1188 a         2400 c         2169 g         1278 t
ORIGIN      
        1 gcagcgcgaa gctcaaatcc acaggctgga gccctcggta caacagcgcc gaagcgttgc
       61 ggtcggcgct ctaacaagcc gctgccccaa ccaaagcact accattctgg ctcaccgtga
      121 gcggcgcaag atcgtggttc acgagatcag attcacgagt ttggccttcg gtatcactcg
      181 ttgcaaagac ttgacgttca gatacgttcc actataaagc acggccatgc agcctgcctt
      241 ttcaacctcg ttgagggtct atgtggcaac cgtactgagc tcaacttcaa cctcgctatt
      301 tgccaccggc ttcgacctgc ccgaccagga tgcctttgcc gcgggtcggg gcatggcctt
      361 cgtggccacg gcggataacc cgtcggccat ctactacaac ccggccggca tcagccaact
      421 ggacggccat catctgcggg gaggcgtgta tggccttcac ctgggcgtcg agtacgattc
      481 gcccgcgggc cgatcgttcg acaacgagaa tccctggcac gccgtcccgc aggtcttcta
      541 cacctacggc ccccagaaac tgcctctgac gttcgggctg ggcgtctttt cgccttttgg
      601 gctaagcatg gaatggccgc aggacaccgg cttccgcacc gtggccaccg aaggcagcct
      661 gacctacatg acgatcaatc cggtcgtggc ttgggcgatc cgcccgaatc tctcggttgc
      721 cgccggtctc acggtcaact acgccgaaat cgatctgcgc caggggttgt tttggccaag
      781 ccagtcctat gatgcgtttc gggtcaaggg tgacggatgg gctctgggct acaacctcgg
      841 cgtgctctgg aaaccatgcg agaaactctc tttcggcgcg tctttccgca gcatcacaac
      901 ccccaacctc gaaggccgca cctcgttccg aaatggcgtc actctacctt tgcccggcgg
      961 cgcagccgtt ccttcgtttg cacaacagaa agtcgatgcc gaggccgatt tcccgttccc
     1021 cctcaagacc atcgtcggtg tttcttaccg ccccaacccg cgctggaact tcgagttcaa
     1081 tgccgactac acggactgga accgcctgaa caccgtgacc atcaagcaag ccgacgcctt
     1141 cccgccactg ctccctcaag atatccctct gacattcgat tgggaatcga gctggtattt
     1201 cgagtggggc gccacccgct acttcgacaa tggctggcat gtcagcggcg gctatatctt
     1261 caaccagaat tcggtcccgg acgcccacta cacccctctg atctcggact tggatcggca
     1321 cttcttcagc gtcggtgcag gacgcaaggg aaaccacatc ggcttcgatc tggcctatca
     1381 attcggttac ggccccaccc gcacggtccg gggcagcgcg ccgtccgcat ccggccagac
     1441 tgccgacggc gattacacgt tcctcagcca tgccgtcatg ctgacgctcg aataccaatt
     1501 ttagcgagag gcgcgtgtca ggccgacgcg gcatgtcatc aattcgcttg cgccggggct
     1561 tggcatggct atcggccttc gacgcctcgc taaaaaaaag gcaccgctgc gcggcgccaa
     1621 taccttgact cgttcagcac agattctgaa aagcaagtca ctcgagcgat ggctcgtcgc
     1681 gtggcgacgc cgcctcggga aaccgctgcc gggcgtccag tttcgactgt cgtggggatt
     1741 tctatgaaaa cgccttcttt ccgttttctc tgcttctggt ctctcctgtg gtctctgctc
     1801 gccggcgggg cggctgcgcc cgaaggtcag gctcagattc tgttgaacgt cgatttcggc
     1861 cccggttcgg cgagtggcaa ggttggcttc gccgcggccg gccagcagac aaatgatttc
     1921 tggaatgcct atggccacta ccagccgaga taccagcctg gcaccgcccc catccccgac
     1981 gggcgtttgg aaggcctgag atacgccgac ggctcggcat cgtcagtcgc cgtggctgtc
     2041 accaatgcgc cgggtgtctg gggcaacgct acgggcgatc cgatgctcga ctcgtacatg
     2101 ttcgccccga acgggtcgaa catggtcgtc acgctcaccg gtctcgacgc cggtcggtat
     2161 cacttctacc tttatggttg ggccgcgccc gatgtggcgg gcgaacaagc ctcgagtttc
     2221 atcctgcgct cagggacgaa tactctcggg cctttgaggg ccccggcgca ggaggatgca
     2281 cccctgctgc acccgggccg cgtgcggatg ccggcggttg tccaacgcga ctttccagtt
     2341 gagacagacc agcccgtggc gatcgaggtc gttccggcgc cggggggcgt ggcggtgctc
     2401 aacgggctgc aggtgctctc ccgcggcacg ggtcctccca ggctggccat gcctgacgcg
     2461 ggtccgtccg cggtgggtct caccaatctg ctggtccact cgatcgccta caccggcgag
     2521 gtcgcggccg ccagcgcgcg gttccaggtt tcgatccatg cgacatcccc gaccacgaat
     2581 catgtcatgg cgccactgtt cgagggcgag ctggctctgc tggatgctca attgcctgat
     2641 ggctggcaga tcgccaggca gggccaccaa ttcaccctgc acacggacac tcccggcagc
     2701 aatcggattg acctgacgct ggtcgcccgg gttacccggg aagaaccttg gaatgtcgtc
     2761 cgcttcaccg ggcctccggc ggcggcggcg acactctccg tcaggtccgc cgacccgcac
     2821 gtggacctcc agcttgtgag tggtgtcctc ctcgagggga gcgacacaac tcagtcggac
     2881 gttcgcggcg tggtgggcgc agaccgccag gttgccatcc gctggcaagc gaaggccgcc
     2941 gagacggcgc gcgaggcttt ggtcaccgtg gacactcggt gctctgtgga cgccacgccc
     3001 gcggccatcc gtctgtcaac cagctttgcg tgcgaagtcc tgcaaggacg atgctcgcga
     3061 atccggatcg ccttgccgcc cggccagacg ctcactaagc tgagcggcga cctgatcaaa
     3121 gactggcagg cggcgtcgga agcgggcctt tcaatcatca caatcgactt cgtccgcccg
     3181 ctcgaaggcg tgaccgcttt cgatgtggtg tcggaacaac cgctgcccgg tttgccggac
     3241 gccgctgaac tgcgtccacc ccagcccctg ggaatccagc gcgagaccgg tcgcctcggc
     3301 gtgcggattg aggacctggt ggcgcgggtg gaccggagcg aggcgctgtg gcaggtcaat
     3361 gccgctgccg gcgagttcgc ggcctttcag ttcaatctgc gtcccgcggt gctccacttg
     3421 catctctcac ccgtcgagcc gctggtgacg gcgagtacgc gggcgcgggc caccgtcgag
     3481 gaaacgcggc tgctggtcga tcatcgagtc gatctgaatg tctcgcgcgc cggcatttac
     3541 ggtctcgaat ggagtctgcc gcccgacttg acggttgcgc gcgtggaagg cgaaggaatc
     3601 gaggactggc aatcgagcgc gggcaagctg cggttgcgat tcgcccaacg cctgctgggc
     3661 cagcgcgccc tcacgatccg cctcgaacag gcgctcaacc ccctgccatc ggagatcatc
     3721 ctgacccctt tgcgcctgac caacaccgcg cgggaaaccg cgctgatcgg cgcgggtgcc
     3781 agcgcgggga ttcaactgaa aaccggcccc ctgagcggcg cccgggaaat cccggtcagc
     3841 ggcttgcccg agcggcggga tgagttgctc gccttccgtg cggacgccgg ggattggcgt
     3901 gtcaacctgg cggtcgaacg tctgagcgcg cggattgtgg ctgaggtgtt caacctgatc
     3961 acgatcggcg acggtctggt cggcggcggc gccacgattc ggtttggcat cgtcaaccag
     4021 ggggtccagc aattccgcgt ccgcatcccg gcccactggc ggaatgtcga gttcaccggc
     4081 gccaacatac gccgcaaaga tcgcgacggc gacatctgga caatcaccct gcaggacaag
     4141 gtctggcgcg gctacaccct ggtcgtgacc tatgaccacg gctttgatcc gcaaaaagcg
     4201 acactggacg cggggggcgc ccacccactc gacgtcgagc gggagaccgg cgcggtcgcc
     4261 atcaccagcg cggccaacct ggccctcgaa ccggcgccga tcgccgagcc cttgaggcgc
     4321 gtcgacccct cggaactggc ggcaaccgat cgcgcgctgg tggcgcggcc ggtgctgttg
     4381 gcctaccgct acgaaggtcc cgcctatgcg ctcgaactcc acctcacacg tcacgaagag
     4441 gtcccggtgc tcgatgccgt cgccgaccgc gcccaattca cgagcgtgtt gaccgactcg
     4501 ggtgagatgc tgacgcaggc gagcttcctg gtgaagaaca acgagcgcgc gcaccagcgc
     4561 ttcgaactcg cccccggcgc gactctctgg ggcgtaaccg tcaatggcga accggtgcgc
     4621 gccgacaggg acggcgattg ggtggttgtg gcgctgccgc gcggcgagaa ccgcgatcaa
     4681 atctatgcca tcgatctcaa gtacgcccag caggtcggcg ccctcggcgg cctgtggccc
     4741 aggcgcctcg aactctctgc tcccaagacc gacgtgcccg gcacctatgc ggagtgggtg
     4801 ctccacgtgc ccaccacccg ccacgtgtac gacttcggag gcaccctcga ggtcaccggc
     4861 ggcaccacct acggctggcg cgacggctgg gaccagttca ttgcgacgta tcgcggactc
     4921 tggcacgagt acggcgccgt gctgatcttc ggcaccggcc tcctggcatt cgtgatcgcc
     4981 ctggtctggt acggcaacag gaagggcttt ggcggcgtcg ccaccgtcct ggtcgtcttc
     5041 tgtttcctcg ccatcctcgc cgggatgctg ctgccggccc tatcgaaggc caagagcaaa
     5101 gcccagcgca ttaattcgct taacaacctc aaacaaatcg gcctcgccgc ccgcctttac
     5161 gcgggcgaca acaacgagcg aatgcccgac agcttcgagg cgatgaggaa cgaactgggc
     5221 accgacaaag tgctcatcga ccctgagacc ggccaacggt acacctacgt cggcgccggc
     5281 aagtccgaag ccgatccgaa tgccatcatc gcctactcgc ccgaacgccc cggcggacgc
     5341 cgcgaggtcg tgttcgccga cggcagcgtg cagatcatgt ccgccgacaa gttcctcgaa
     5401 gccaccgccc agcaccagac cgtgccgatg gatcagcggg tgttcgagcg taacggcctt
     5461 gccgggcgtc cgcaggttgc cccagtcact cccccggccc agacgggcat cgcaattccc
     5521 cagccgacac cgccccagca ggtccctgta gcccctgcga ttcaagcgcc gacccaagcc
     5581 cccgcggacg ctcctgaggc gatgccggtc ttcgacgcga ccgggcccac ggcggccggc
     5641 atcaagtcga tccgcttcga ccttcccaag gcgggacggg cctacacgtt cacgcgtgtc
     5701 ctgagcgtcg aggaaaaggc ccctcggatc agtgtcggcc tggtgtcgag ccgactcttc
     5761 acatttgtcc gtgccgccgt ccaggtgctg gctttcctgt caggcctgat gcttgtgctg
     5821 gtccagtggc ggtcggccca tcctaaagcc ctctggttgt cggtcgggct gatgctcgcg
     5881 ggtctggcaa ccgccgatct cttgatcgct tggcgtgttt tgcacctggc cctcaccctg
     5941 gccgtccctg tcctcctggc cttgctgcct gcgtggctgc tgctgcgctg gcgtcaacac
     6001 gccaagagca accgcccgac tccagcgtcc ccgcccgatg cttcgccggg aaccgacatc
     6061 gccgggacaa cctcggtcgc cgggtcggct gtgctggcgc tggtgttgct aaatggaggt
     6121 ccggaggccc tcgccgcctc gtcgctccag ccgctttcca gccgcgtgac gatcacacgc
     6181 ctcgatctga ccggcagcgc cagcccgcgg gccgcccaat tcgacgccac gctcctgctc
     6241 gcggccaacg gcaccaatgc attcgctcag ttgttcggct ctgacgttgc ggtccaggaa
     6301 tcgaccgtca tgaccggcga ggcgcagttc tggcgcaacg gcaatgcatg gagcgtgttc
     6361 atgccacacc ccggctccgc cacgctgcga ttgaaactgc tggcgccctt gggcggcgac
     6421 ctggccaaac cggaactcga tctggcgctg ccgcccgcct tgggcgggca gatggcgctt
     6481 gtgctttcgg agcccgacgc cgatgtcgag tttccggatg cgatcggctt ggtacgacgg
     6541 ctcgagggcg agacgaccgt gatcgaggcc gtactgggga gcaacgatcg gttgaatctg
     6601 cgctggacac ccaagcgcaa acgggccgac gaggtcgagg cgacggtctt cgccgatcag
     6661 accgcgctca tcacgctcgg caacggagcc attcacacac gcaccgtgat agattggcaa
     6721 gcgagccagg gcgaactgcg ccaggtgtgc gttcggcttc cgtctggctg tcggctcctc
     6781 aaggtgcaag gtccgcacgt tcgttcctgg cagttggcgc cgacaaacgc cgacacgctc
     6841 gtggtcgacc ttctcaagcc cgtcgccccc ggcttgcgac tcaccctcga acttgaaaag
     6901 actctggatg ccctcccggt cagcgcgcgg attgccgtgc ctgaggcgct cgatgtgaag
     6961 cgccagaccg gcagcatcgg cgtccgggcc gcggcggaac tgggtttggg cgtcgagcgc
     7021 gcgcgtgggc tcgac
//
LOCUS       DAOQRO010000165         6999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10574_length_6999_cov_4.774050, whole genome shotgun sequence.
ACCESSION   DAOQRO010000165 DAOQRO010000000
VERSION     DAOQRO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10574_length_6999_cov_4.774050"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(488..1027)
                     /locus_tag="PKK20_07460"
     CDS             complement(488..1027)
                     /locus_tag="PKK20_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99762.1"
                     /translation="MNATLHPHRPVSRLAPRRRQSARGLIGWAFALFLAAEGGAASWD
                     PTAEDYSGREGTTLYVSKLGDNAVGLSYASYCARVQLVRCRLIVLNFTQPEMDGKSSG
                     IICTQGHRAEGRLHVDLEDCMLAGYSVLTASKDGEAASYTTKGRVQTYVQYRQPSTFS
                     QNGQSARTTSVFKGQTHVG"
     gene            1153..4488
                     /locus_tag="PKK20_07465"
     CDS             1153..4488
                     /locus_tag="PKK20_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNU99763.1"
                     /translation="MKRRIPIQAQSGTKAVLVYYWGAILMGLAPGAFAESPAGSPARL
                     ARQPDIHGDLIVFVHGEDIWTVASAGGVAKRITFHEGEERFPRFSPDGRRIAFTGEYD
                     GNADVYVMNVEGGDISRVTYHPGPDEVVGWHPTNGKILFRSTRDSYSRFTRLYLIAPD
                     GGGLEGLPLPEAGWGSFSPDGSTIAYTRSATEDRSWKRYRGGLAPDIYLYDFNTGTDR
                     RLTDGRGTERFPMWIGEEIYYQSDAEGLLNLWAINPRTGLARQLTHYADFEAGHPSEG
                     GGLIVYDRAGRIEVFEPATGQARVLPLEILAEAPETRPYVRNVKDFVTQIGIAPDGSR
                     ALVIARGEIFSVPRDKGITRNLSQDSGSRDKDAAWSPDGRHIAYFSDRAGEYELWLTD
                     PLGKEQTARLTTHADGYRHSLRWSPDSKKIAFSDQTLTLYCLDVESREIVKIDRAEHE
                     AMDLGIDAKPICDHAWSGDSRFLAYSKIGPDLVSRVYVHCLETKETRAVTGGPLDSFD
                     PVFSEDGQRLFFVSNRRFDPTYCDMDFELVYKKVAGLYAIALRKDTPALLAPETGDDA
                     GSRDAADGKGQDEAEGKKEDKADTPGAAKLTIDFDGIEQRVEPLPLPGGNYRHLGFAK
                     GALFFLDCDKGDFNRFDIREPGPRKLRAFDAAKRAARTVIESVHDYRISADGNRIVWR
                     RGTDVGIVDSSAENARAEPLDLSGLTLKLDPRAEWAQVYREVWRHERDFFYDPGMHGL
                     DWHAVGEKYRPLIEAASSRAEVRWIIGELIGELGTSHTYVYGGDRRRKADRVSVGMLA
                     VDWEVDASANRWRIRRILRSPDWITGAVPPLAVPGVDAREGDYLVAVDGRAVTAGREV
                     YAAFEGLAGKPVTLTLSDKPGGDSARDVLVVPAESELRFRYLDWVEGNLRKVEQASNG
                     RIGYIHLPDTFLSSAAMFPLYWYGQTQKEGLVIDGRYNGGGLDPDVFLRRLDAPISFY
                     WTRRHSTDYSTPLVATRAHMALLTNRQAGSGGDMLPAEFRLRKMGPIIGTRTWGGLVG
                     ISMFISLVDGGGITAPDYRVYSTEGKWVIENEGVEPDIPVDLDPAEMARGHDAQLMTA
                     VDFLKQKMAETSRPVPPRPPSVR"
     gene            4573..5001
                     /locus_tag="PKK20_07470"
     CDS             4573..5001
                     /locus_tag="PKK20_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNU99764.1"
                     /translation="MSRVTKLLHTRYRLNDLERSVQFYKKVLGLTEVRRHKSPRGSEL
                     VFLKAPDSEELVELCHFPDSGPVEVQPDLTHLAFEVSSLKEFGQHLAKHGLKYSDGPH
                     MRDDGSGFAFIDAPEGYEIELMQRPTQFLLAGQEGKSGGC"
     gene            5318..>6999
                     /locus_tag="PKK20_07475"
     CDS             5318..>6999
                     /locus_tag="PKK20_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNU99765.1"
                     /translation="MSAYLYSYLVGGVFFGIGLGYAAKHGYVGFTGRKLRNLLMSLGV
                     ILFFLVLQGYLQFAPMSEAPARAYTGGAEAVLKDVGHIRGTPIDYGIVLAYFCLILFN
                     GLYFGRRQKTTKDFFFGGQRFAWWLIAISLIATTIGSYSFVKYSEIAYKYGLGASQAY
                     WDDWLWFPLLAFGWLPLLYFSRIVSVPEYFGRRYGSKVRLWATLYMLVYLVGYVGVNL
                     YTMGVIVNILVGWPISLAAMVVAAISATYVTVGGQSSVIMNDLVNGLFLLLVGLLVIV
                     LGAVYLGGFDALWLNLPREARLAFPNFNEDPEFPAVGIFWQDAFANSAMFYFLNQGII
                     MRFMAAKSLDESRKAATVMVVALMFIGALVVSSGGLVARAFTHAGVLPEVPAREAFFI
                     ATEFLSHPGIFGLILAAMTAALMSTVDTLITAVSAVTVNDVYRPYFRPEATDRQLLRV
                     ARIAALCVTVFGVSMVPVFMQFKSIYVAHAAFTAAVTPPMVIALLFAVFWRRFTGAAA
                     LSTLVGGLALMALSMLLPAVIVPFAHGVPGGDTAWDFLAGMREQQFMRAFYGV"
BASE COUNT         1194 a         1999 c         2374 g         1432 t
ORIGIN      
        1 gactcttttc ggcccttgaa ggggttccgc ggctgatggc ggagctcatg tatggggcta
       61 gtaaccccgt agtcaggcag tacgcgccag cagtggccta ttacgcttga gtagccttcg
      121 gctttgtgcc aatttgtctc ggaaaaccac cgtgtgtggt tcgattccga ccagggcacc
      181 ggttttgcgt ggcgaggaat gcaactggct ttacggtacg ctgtgcgcgc cgcacgactc
      241 gatcgatgcg cgtagatgca gatcgacccg tccaacgatc tgtcctgcgg ccgataagga
      301 acgaccggcg gtgattcccg aagtcgtgcc ccagtcaaac gagtcgcccc agggttggct
      361 accggtacgc tgacggactc tgcgccatgg ttggcgagct tgtgcggaaa cagcccggga
      421 ggtccttgcc tacacttgcc tacagcaggc gttgcgaaac gccgcatcat cgtgtcgtcg
      481 ttggcaatca gccaacatgc gtttgaccct tgaaaacact ggtcgttctt gcgctttgtc
      541 cgttctgcga gaaagttgag ggttgtctgt attgcacata cgtctggacc cggcccttgg
      601 tggtgtaact ggcggcttcg ccatccttgc tcgcggtcag cacgctgtaa cccgccagca
      661 tgcaatcctc caaatccaca tgcagacgcc cctccgctcg atgtccttgc gtgcagatga
      721 tgccgctcga tttcccgtcc atctccggct gggtgaaatt caggacaatg aggcggcagc
      781 gcacgagctg gacccgggcg caatagctgg cgtacgagag cccaaccgcg ttgtcgccga
      841 gcttcgagac atagagtgtt gttccttcgc gcccggaata atcctccgct gttgggtccc
      901 aggatgcggc gccgccttca gccgccagga acagggcgaa tgcccatccg atgagaccgc
      961 gcgcggactg gcggcggcgt ggtgcgagtc gggacactgg cctgtgcgga tggagagtcg
     1021 cgttcatgcg cgccagtcac tcacaccgcg gggagggatg gaaagtcaaa accggtcatg
     1081 cggcgaatcc ctggtggcgt gctgcccaat aggccttgaa caggctcttt cgtttgggca
     1141 atcatctgcg gcatgaaaag gcgaatcccc attcaggcgc aaagcggcac caaggccgtg
     1201 ctggtttatt attggggagc gattctgatg ggcttggcac ccggagcttt cgcggaatct
     1261 cccgcgggct ctccggcgcg tcttgcacgg cagccggaca ttcacggcga tctgatcgtc
     1321 tttgttcatg gggaggacat ctggacggtt gcgtcggctg ggggcgtggc gaagcggatc
     1381 acgttccacg agggagagga gcgtttcccg agattctcgc ctgacgggcg ccggatcgcg
     1441 ttcacaggcg agtacgatgg caatgcggat gtgtacgtca tgaacgtcga ggggggcgac
     1501 atttcgcgtg tgacctatca ccccggaccc gacgaggttg tgggttggca tccgacgaac
     1561 ggcaaaatcc tctttcgctc gacgcgtgac tcctacagtc gcttcacgcg cctctatctg
     1621 attgcgccgg acggcggcgg tcttgaaggg ctgccgctgc ccgaagccgg ttggggcagc
     1681 ttcagccctg acggatcgac gatcgcttac acgcgcagcg ccaccgagga tcggtcgtgg
     1741 aagcgctatc ggggggggct ggcaccggac atttacctat acgatttcaa cacaggcacg
     1801 gatcggcggc tcacagacgg gcgtgggacg gagcgattcc ccatgtggat cggggaggag
     1861 atctactacc agtctgatgc ggagggcctt ctgaacctgt gggcgatcaa cccacggacc
     1921 ggcctggcga ggcaactcac gcactatgcg gatttcgagg ccggccaccc ttccgagggc
     1981 gggggactga tcgtttacga ccgtgctggg aggatcgagg tgttcgagcc tgccacgggg
     2041 caggcgcgcg tcctcccact cgaaatcctg gccgaggctc cggagacccg gccatacgtc
     2101 cggaatgtga aggacttcgt gacccagatt ggcatcgcgc cggatggcag ccgggcgctg
     2161 gtcatcgccc gcggagagat tttttccgtg ccgcgcgaca aggggattac ccggaatctg
     2221 tcgcaggatt ccggatcacg ggacaaggat gcggcgtggt cgcccgacgg ccggcacatc
     2281 gcgtacttct ccgatcgagc cggcgagtac gaactgtggc tcaccgaccc gctcggcaag
     2341 gagcagaccg cccgcctgac gacgcacgcc gacgggtatc ggcacagcct gcgctggtcg
     2401 ccggactcga agaagatcgc cttctccgat cagacgctga ccctgtactg cctggacgtc
     2461 gaatcaaggg agatcgtcaa gattgacaga gcggagcatg aggccatgga cctcgggatc
     2521 gacgcgaagc cgatctgcga tcatgcctgg tcgggcgaca gccggttcct ggcgtactcg
     2581 aagatcgggc cggatctcgt gtcgcgagtt tacgttcact gcttggaaac gaaggagacg
     2641 cgggcggtga caggcgggcc gttggacagc ttcgatccgg tgttttcgga ggatggccag
     2701 cgccttttct tcgtcagcaa tcgtcgcttc gatccaactt attgcgacat ggatttcgag
     2761 ctggtctaca agaaggtggc tgggctttat gccatcgcgc ttcgcaaaga cacgcccgcc
     2821 ctccttgcgc ccgagaccgg ggatgacgcc gggtcgcggg acgcggcgga cggcaagggg
     2881 caggatgagg cggagggaaa gaaagaggac aaggccgaca cgcccggggc ggccaagttg
     2941 accatcgact tcgatgggat cgagcagcgt gttgagcctt tgcctctgcc cggcggcaac
     3001 tatcgccacc tcggcttcgc caagggcgcg ctgttctttc tggactgtga caagggggat
     3061 ttcaaccgtt tcgacattcg cgaaccgggc ccacgcaagc ttcgggcatt cgacgcggcc
     3121 aagcgggcgg cgcgcacggt catcgagtct gtgcacgact accgcatctc cgccgatgga
     3181 aaccggatcg tctggcggcg cggaaccgat gtcggcatcg tcgactcgtc cgcggagaat
     3241 gccagggctg aacccctgga tttgtccggg ctgacgctga agctggatcc ccgcgccgag
     3301 tgggcgcaag tgtaccgcga ggtttggcgc catgagcggg acttcttcta tgacccgggc
     3361 atgcacggtt tggactggca cgcggtgggg gagaagtacc ggccgctgat cgaggcggct
     3421 tcgagccggg cggaggtgcg atggatcatc ggcgaactca ttggcgaatt gggcacttcg
     3481 cacacatacg tttacggggg cgatcggcgg cggaaggccg accgggtgtc agtgggcatg
     3541 ttggcggtcg attgggaagt cgatgcctcg gcgaatcgtt ggcggattcg gcgcatcctg
     3601 cgttcgccgg actggatcac gggggctgtg ccgccgctag ccgtgcccgg ggttgatgct
     3661 cgggagggag actatctagt ggctgtcgac ggccgggcgg tgaccgctgg gcgcgaggtg
     3721 tatgcggcct tcgagggttt ggccggcaag ccggtgacgc tgacgctgtc ggacaagccg
     3781 ggcggagact cggccaggga cgtgctcgtg gtgccggcgg agagcgagtt gaggttccgg
     3841 tatctcgact gggtggaagg caacctgcgc aaggtcgagc aggcgtcgaa cggaaggatc
     3901 ggctatattc atctccccga tacattcctg agttccgcgg ccatgttccc actctactgg
     3961 tacgggcaga cccagaagga agggcttgtc atcgacggcc ggtacaacgg gggcgggctt
     4021 gatccggacg tgtttcttcg gcggttggac gcgccgattt cgttctactg gacgcggcgg
     4081 cattcgaccg actactcgac gccgttggtt gcgacgcgag cgcatatggc gctgctcacg
     4141 aaccggcagg caggctcggg gggcgacatg ctccccgccg agttccggct caggaagatg
     4201 ggtcccatca tcggcacacg cacctggggt ggcctggttg gaatctcgat gttcatctct
     4261 ctcgtcgacg gcggcggcat cacggcgccg gactatcgtg tctacagcac cgagggcaaa
     4321 tgggtcatcg agaacgaggg cgtcgagcct gatattccgg tcgatctcga tcccgcggag
     4381 atggctcgag ggcacgacgc gcagctcatg acggcggttg acttcctgaa gcagaagatg
     4441 gccgagacgt cccgacccgt cccgccgaga ccgccgagtg tccggtgaga acggcttggg
     4501 cggcctgggg tggcaggggt aggacgtggc ctatcattgc gcccgcctaa tcctcgagct
     4561 acgctgggcg ccatgagtcg cgtcacgaag ctcctgcaca cccggtatcg gctgaacgat
     4621 ctcgaacgaa gcgttcagtt ttacaaaaag gtccttggtt tgaccgaagt ccggcggcac
     4681 aaatcgccgc gcggttccga gctggtattc ctgaaggcgc ccgacagcga ggaactcgtc
     4741 gagttgtgtc acttcccgga cagcggcccg gtcgaggtcc aaccggacct gacgcacctg
     4801 gccttcgagg tcagcagcct gaaggagttt gggcagcatc tggcgaagca cggactcaag
     4861 tactcggacg gcccgcacat gcgggatgac gggagcggct ttgcgtttat cgatgcgccg
     4921 gagggttacg aaatcgagtt gatgcagagg ccgacgcaat tcctattggc tggacaggaa
     4981 gggaagtctg gcggctgcta acccgcatat tccgcacccc gtcggtgcac agggcgatca
     5041 ggactgcgag tgttttcatg ggccaagtct ggcgattgac ggcaaaactg cgcgcctggc
     5101 ccgtcgagcc aacaagccca aagtcggcgc gttaaacaga tgcgacgctc cattggccac
     5161 gcgggtggca gttccggagc aaggcagtgg gcgggccagg agggcggacc ttggcgtgat
     5221 gtgcgccggc catgctgccc tgcgccatcg tggtgccgcg tttctggtgt ttgctttcgc
     5281 agggggaatt ggatttgaat ggccggggaa accgacgatg agcgcatacc tttattctta
     5341 tctcgtgggt ggggtgtttt tcgggattgg acttggctac gcggccaagc acggctatgt
     5401 gggattcacc ggtcggaagc tccggaatct gctgatgagc ttgggggtga tcctgttttt
     5461 tctagtgctg caggggtatc tgcagtttgc gccgatgagt gaggccccgg cgcgcgccta
     5521 cacgggcggc gctgaggcgg ttctgaagga tgtgggccac attcgcggca cgccgatcga
     5581 ttacggcatc gtgctggcgt acttctgcct gattctgttc aacgggctgt atttcggacg
     5641 ccgacagaag acgacgaagg actttttctt cggaggacag aggtttgcgt ggtggttgat
     5701 tgcgatcagc ctgattgcca cgacgatcgg ctcgtacagc ttcgtcaagt acagcgagat
     5761 cgcgtacaag tacgggcttg gagcctcgca ggcctactgg gacgactggc tctggtttcc
     5821 gttgctggcg ttcgggtggc tgccgctgct ctacttttcg cggatcgtgt cggtgcctga
     5881 gtacttcggg cgtcgctacg ggtcgaaggt gcgtttgtgg gcaactcttt acatgctggt
     5941 gtacctggtg ggttacgtgg gggtgaacct gtacacgatg ggcgtgatcg taaacatcct
     6001 ggtcgggtgg ccgatctcgc tggcggctat ggtggtcgcg gcaatctcgg ccacctacgt
     6061 cacggtcgga gggcagagca gcgtcatcat gaacgatctg gtcaacggcc tgtttctgtt
     6121 gctggtgggc ctgctggtga tcgtgctcgg cgcggtctac cttggcgggt ttgacgcgct
     6181 ttggctgaac ctgccgcggg aggcgcggct ggctttcccc aatttcaacg aggatcccga
     6241 gtttcccgcg gtcggcatct tctggcagga cgcctttgcg aactcggcga tgttctactt
     6301 tctgaatcaa gggatcatca tgcgcttcat ggcggccaag tcgctggatg aatccaggaa
     6361 ggccgccacc gtcatggtgg tggctctgat gttcatcggt gccctggtgg tctcttcggg
     6421 cgggttggtg gcgcgcgctt tcacgcacgc gggcgttctg cccgaggttc cggcccgcga
     6481 ggcgttcttc atcgccaccg agtttctcag ccatccgggc atcttcggtt tgattctggc
     6541 ggccatgacg gccgcgctga tgtcaacggt ggacacactc atcaccgcag tctccgcggt
     6601 gacagtgaac gatgtgtatc gcccctattt ccgccccgaa gccacggatc ggcaactgct
     6661 gcgtgttgcc cgaattgccg ccttgtgcgt gacggtcttc ggggtgtcga tggtgcccgt
     6721 gttcatgcag ttcaagtcga tttacgtggc gcacgcggcc ttcacggcgg cggtgacgcc
     6781 cccgatggtg atcgcgctgt tgttcgcggt gttctggcgg cgattcacgg gcgccgccgc
     6841 gctcagcacg ttggttggcg gtttggcgct catggccctt tcgatgctcc tgccggcggt
     6901 tattgtgccc tttgctcatg gggtccccgg aggagacact gcctgggatt tcctggccgg
     6961 gatgcgggag caacagttca tgcgcgcttt ctacggcgt
//
LOCUS       DAOQRO010000166         6979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10616_length_6979_cov_4.099798, whole genome shotgun sequence.
ACCESSION   DAOQRO010000166 DAOQRO010000000
VERSION     DAOQRO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10616_length_6979_cov_4.099798"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PKK20_07480"
     CDS             complement(<1..879)
                     /locus_tag="PKK20_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNU99766.1"
                     /translation="MKPPTSIDSRPARASATLSRATRANRRARQWLPHLAGLVLIGAL
                     LFALWPQPVRVETAPAVWGPLRVSVNEEGKTRIRHRYVVSAPVAGHLRRTVLKAGAPV
                     EAGVTKIAVIDPLDAALLDPRSRALAAARRDTAAANLEKAREAHRFASSELRRFETLA
                     REQAVSPQELESAQWRQASASREESAAEGALRVAEAELAVFATPASESGQATHSPLTV
                     LAPATGRVLRVFEESSRPVSTGTPILEIGDPNDLEVVIDVLSRDGAVLESGTRIELEQ
                     WGGPEILEATLRLVEPA"
     gene            complement(887..3253)
                     /locus_tag="PKK20_07485"
     CDS             complement(887..3253)
                     /locus_tag="PKK20_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNU99767.1"
                     /translation="MLPCLDRKLFRDLNRLCGQAMAVALVMACGLAMMIMARSLIRSL
                     ETARDDYYQDHRFAQVFAHLKRAPRSVADQAARIPGVARAQADISVQVTLDIAGLDEP
                     ASGTVRSLPAFGQPELNRLFLRAGRWLSPGRHGEVLVGEAFAEANRIRPGDSIIMVLH
                     GRREVLRVAGIVLSPEFIFESRPGTPLPDSRAYGSFWLPYADLAAAFSLDGAFNYMVL
                     TLEPGASERAVIAELDRLFKPYGGRGAFGRAEHPSHIRVLDEIRVLATMAVAFPIVFL
                     GVAAFMTNAILSRLVDLQREQIAILKAFGFTNRQVAAHYFKFALVIVAGGTLTGAIGG
                     ALLGQWLVGLYHNFFRFPELTFRLDRPALLAALIVSAAAAMTGVFGAVRRAARLPPAE
                     AMRPQPPASYRPAWIERGRMARHLSATFRIAMRNIERRLMRALFTCAGLSLATGILIV
                     PNCFRDAVAEVLDYQWHVVQRQDMSAGLIEPISLAARHPLRQLPGVISVEPVRSVAVR
                     LRFGHRHRQLILRGLPAKGLHDRIINPDYQTIILPPQGLVLSAKLAQILGATVGDQII
                     VEVLEERRPVRSVPLVRLAEDFAGLAAYMERSALNRILGEGDVINGATFRVDPGHRAA
                     FLHALKETPRVNWVAIKDSMRANFRQTTAASINTFQMIYLVFATVVAFGVVYNNVRIS
                     LAERARELATLRVIGFSRREVGGVLVTELVMLALVAVPFGLLLGSGFAAGIVRAINTE
                     TVRLPVVLTMGNFGFAVLVVSVASTLSAIFVLRQLHRLDLVGALKAPE"
     gene            complement(3258..4154)
                     /locus_tag="PKK20_07490"
     CDS             complement(3258..4154)
                     /locus_tag="PKK20_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99768.1"
                     /translation="MGMRFASTHGFGNAKVCDVNPPDATRSSIIGRDNQSEHAKERMD
                     DRAPVSDTVTRTAQASSGSEPALVYRARKVTKVYGSGPAQVHALAGVDLDLFEGELVV
                     LLGPSGSGKTTLLNNLGGLDLPTAGTLEYRGADLTAVDESQLTQFRRECVGFIFQFYN
                     LIPSLTARENVALITEIATDPMPPEAALEMVSLGARLDHFPAQLSGGEQQRVAIARAI
                     AKRPAVLLCDEPTGALDVRTGIVVLEAIARVNRELGTLTVIITHNAVMADMADRVIFL
                     SDGRVQQIRRNARRAPVNSLKW"
     gene            4299..6182
                     /locus_tag="PKK20_07495"
     CDS             4299..6182
                     /locus_tag="PKK20_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99769.1"
                     /translation="MNRASIFPQWCALLLGLGPVLFQPLAQVDQLAYRAVRSSAFEVQ
                     PTDAPSSIQGPFRVRGSFSMAPQIAPLDWENYRISDLRLEVLLDAAPPAVLRGSGTFR
                     RSGLFNRMRMDLDVQLGETALHLTSGELPAEPWTPDIDMVLKGKAVDGTRPGTYALRL
                     AAVRELARWQYRLIDGSSWTDDCEKCGRPTIRWSLHGGFDLVLVEKHPLFNRYHLFDI
                     RFFSGTDYELEGEGDYQVGGEVALRQELRLDVEAKGTFGGPRTMTLTNMPGPPTRLWP
                     MWAVDVQEAESTPESKLLLELRAAPVRELWFSTTHGMTAGLQPGLQPRISGGDVLTDS
                     GRRVKTNAQLISALGWTEPKEALAVDALTIAPGGEVWFSFSEGQTSPTLGVISDGDLV
                     SDHGRIVRRNGELMASFGLMPPLPDLGLDGVQVLDDGEILFSIVRESFSERLGVMLRP
                     GDILSSRGNVTRAAKELLAVFEPEDQAADPGLDAFFVWPSGEVWFSTESGFISKTMGP
                     IMDGALLSDQGYVVAHNLDLVQPFQPLEDLANFGLDGLFLVTDVAAPAKPARVLLSLK
                     NGEGGGVRLTWPAQGRVFQVEATTAVGDPFAPVSPIVPDLEWMDSSASGLTQSSYYRL
                     RQW"
     gene            complement(6329..>6979)
                     /locus_tag="PKK20_07500"
     CDS             complement(6329..>6979)
                     /locus_tag="PKK20_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99770.1"
                     /translation="PGLSELEWQFSTQYHFVWLSGDDVVQSLVHNMDRASWALQEQAP
                     VKCHGLGGRSSMTDAIYGDVFDHHTVVYQYENGARVYALCRTTTGCYDEYSSLIFGTK
                     GRADVMACRIWGEKNWRGDAGCDAHYQEQVEYMKAIRAGTPINNGDYMAPSTLIGIMG
                     QISCYTGKEVTWEQITASDFFFPPRPEDCRDGMEPPTKPGPNGSYPVYIPGRSRLI"
BASE COUNT         1270 a         2150 c         2342 g         1217 t
ORIGIN      
        1 cgcgggttcc accaggcgca gcgtcgcttc gagaatctcc ggaccacccc attgttcgag
       61 ttcgatccga gtgcccgact cgagcaccgc cccgtcgcgc gagagcacgt cgattaccac
      121 ctcgagatcg ttcggatccc caatctcgag aatgggtgtt cccgtcgaca caggccggct
      181 gctctcttcg aacacccgca gcacgcggcc cgtggccggc gccagaacgg tcaagggcga
      241 atgggtcgcc tgaccggatt ccgatgctgg cgtggcaaag accgccaatt cagcctcggc
      301 cacccgcagg gcgccctcgg ccgcggattc ctcccggctt gcggaggctt ggcgccactg
      361 ggcggattcg agttcctggg gtgagacggc ctgttcgcgt gccagggttt cgaagcggcg
      421 caattcgctc gaggcaaagc gatgggcttc gcgcgccttt tcgagattcg cggccgccgt
      481 gtcacgccgc gctgcggcca gagcgcggct gcgcgggtcc aaaagagcag cgtcaagtgg
      541 gtcaatgacc gcgatcttcg taactcccgc ttcgaccggg gcgcccgcct tcaggaccgt
      601 gcgccgcagg tgtccggcaa cgggggcgga gaccacatag cgatggcgaa tgcgcgtctt
      661 gccctcttcg ttgacgctga cgcgcagcgg tccccagacg gcgggggcag tctcgacgcg
      721 gactggctgg ggccaaaggg cgaagagcag ggccccgatg agaaccaatc cggccaggtg
      781 gggcagccat tggcgagccc gccggttcgc gcgggtggca cggctcaggg tggccgatgc
      841 gcgggcaggg cgcgaatcga ttgacgtggg tggtttcatt gggtggtcat tcaggggctt
      901 tgagcgcgcc aacaagatcg aggcggtgga gctgacgcaa aacaaagatg gcggaaagtg
      961 tcgaagcgac cgaaaccacg agcacggcga acccgaaatt gcccatcgtc agcaccacgg
     1021 gcagacggac ggtctcggta ttgatggccc tgacaatgcc ggcggcaaag cccgagccca
     1081 gcagcagtcc gaacggcacg gcaaccaggg cgagcatgac cagttcggtc accagaactc
     1141 cgcccacttc gcgtcgggag aagccgatca cccgcagcgt ggccagttcg cgggcgcgtt
     1201 cggcaagcga gattcggacg ttgttgtaga ccacgccgaa ggccaccacc gtggcaaaca
     1261 ccaggtaaat catctggaac gtgttgatgc tcgccgccgt cgtttgccgg aagttggcgc
     1321 gcatcgagtc cttgatcgcc acccagttga cgcgcggcgt ctctttcaac gcgtggagaa
     1381 acgcggcgcg atggccgggg tcgacgcgga aggttgctcc attgatcacg tcgccttcgc
     1441 ccaggatgcg attgagggcc gaacgttcca tgtaggcggc caggcccgcg aagtcctcgg
     1501 ctagtcgcac cagcggaaca gagcgcacag gccgccgttc ctcgagcact tcgacgatga
     1561 tctgatcgcc caccgtggcg ccgagaatct gcgccagctt cgcggacagc accagcccct
     1621 gtggcggcag gatgatggtc tggtagtcgg gattgatgat gcggtcgtgc aggcctttgg
     1681 ccggaaggcc tcgcaggatc aactgccgat gccggtgccc gaagcgaagg cggacggcga
     1741 cactgcgaac gggctcgacg ctgatcacgc cgggcaactg gcgcagagga tgccgggcgg
     1801 caaggctgat gggctcgatc aggccggcac tcatgtcctg gcgctggacg acgtgccatt
     1861 ggtaatcgag gacctcggcc acggcgtctc gaaaacaatt gggcacaatg agtatgccgg
     1921 tggccaaaga caggcccgcg cacgtgaaca gcgcccgcat gagacgacgc tcgatattgc
     1981 gcatcgcgat gcggaaggtc gcggagagat gccgtgccat tctgccgcgc tcgatccagg
     2041 cgggacggta cgaggcgggc ggttggggac gcatggcctc cgcgggaggc aggcgtgccg
     2101 cgcgccgcac ggcaccaaac acgcccgtca tcgccgcagc ggcgctcacg atcaacgcag
     2161 cgagcagagc cggacggtcc agacggaacg tcagttctgg gaaccggaaa aagttgtggt
     2221 aaagtcccac cagccattgg cccaggagag cccctccgat cgcgccggtc agcgtgccgc
     2281 cggcgacgat cacgagcgcg aacttgaaat agtgggcggc gacctgtcga ttggtgaagc
     2341 cgaaggcctt gagaatggca atctgctctc tctggagatc gaccaggcgc gaaagaatgg
     2401 cgttggtcat gaaagccgcg acaccgagaa agacaatcgg aaaggcgacc gccatcgtgg
     2461 ccagcacccg gatttcgtcc aggacacgga tatgcgacgg atgctcggcg cggccaaagg
     2521 ccccgcgtcc gccgtaaggt ttgaagagcc gatccaactc ggcaatgaca gcgcgttcgg
     2581 acgcgcccgg ttcgagagtg aggaccatgt aattgaaggc gccatcgagg ctgaaagcgg
     2641 ccgccaaatc ggcgtacggg agccagaagc tgccgtaggc gcggctgtcg ggcagagggg
     2701 tgccaggacg ggattcgaag atgaactcgg gcgagaggac gatgccggcg acgcgcaaga
     2761 cctcgcgtcg gccatgaagc accataatga tcgaatcgcc cggacgaatc cggttggcct
     2821 cggcgaacgc ttcgccgaca aggacctcgc catggcgtcc cgggctcagc cagcgtccgg
     2881 cgcgcaggaa aaggcggttc aactcaggct ggccgaatgc cggaagcgaa cggactgtgc
     2941 cgctggccgg ttcatccagg ccggcaatgt caagggtgac ctggacggag atgtccgcct
     3001 gtgcgcgggc gacacccgga atcctggctg cctggtcggc gacggaacgc ggggcccgtt
     3061 tgagatgcgc gaacacctgc gcgaagcgat ggtcctgata gtagtcgtcg cgcgccgttt
     3121 cgagcgatcg gatcaggctg cgcgccatga tcatcatcgc cagcccgcag gccatgacaa
     3181 gggcgacggc catggcctgg ccgcacagcc ggttcagatc gcggaacagt ttgcggtcaa
     3241 gacagggcag catccgatca ccatttcagg ctgttcaccg gggcgcgcct ggcgttgcgc
     3301 cggatttgct gaacgcgtcc gtccgacaga aagatcaccc gatccgccat gtccgccatg
     3361 acggcattgt gggtgataat gacggtgagt gtgcccagtt cgcggtttac gcgcgcgatc
     3421 gcttcgagaa caacaatgcc ggttcgcaca tcgagggcgc ccgtcggctc gtcgcacaac
     3481 aggacggcgg gacgcttggc gatggcgcgg gcaatcgcca cacgctgttg ctcgccgccc
     3541 gagagctgcg cagggaaatg gtccagacgc gccccgaggc tgaccatttc aagggccgcc
     3601 tcgggcggca tcggatcggt ggcgatctcg gtgatgaggg cgacgttctc gcgtgccgtc
     3661 aggctcggaa tcaagttgta gaactggaaa atgaacccta cgcattcgcg gcggaactgc
     3721 gtgagttgcg actcgtccac cgccgtcagg tccgcgccgc gatactcgag tgtgccggcc
     3781 gtgggcagat caaggccgcc aaggttgttg agcagtgtgg tcttcccgct gcctgacggc
     3841 ccaagcagca ccaccaactc accttcgaac aggtccagat cgacccccgc cagggcatgc
     3901 acctgggcgg gacccgagcc atagaccttg gtcaccttgc gggctcgata gacgagcgcc
     3961 ggctccgacc ccgaggacgc ttgcgccgtt cgagtcaccg tatcggacac tggggccctg
     4021 tcatccatcc tctctttcgc gtgttcggat tggttgtccc tgccgatgat cgaactccgc
     4081 gttgcatcgg gcgggttgac gtcgcacacc ttcgcgttcc cgaagccgtg cgtcgaggca
     4141 aaacgcattc ccattgtcgg gcgcgctaaa cggctcgctc ctctctcggc cagggactcc
     4201 cgttcctgtt ttgcgcgctg gcggcgcggg cagggatgtg gtatgtaaca ggcgtcttgc
     4261 caaaggcact gccagaaacg atgcctgagg ataacgctat gaatcgtgcc tcgatctttc
     4321 cgcaatggtg cgcgctgctg ctcgggcttg gccccgtgtt gttccagccg ctggcccaag
     4381 tcgatcaact cgcctaccgc gccgtccgat cgagcgcgtt cgaggtgcag ccgaccgacg
     4441 ccccttcgag cattcaaggg cctttccgcg tccgcggcag cttctcgatg gctccccaaa
     4501 ttgcgccctt ggactgggag aactacagga tttcagatct ccgactcgaa gtcctcctgg
     4561 acgcggcacc cccggccgtc ctgaggggca gcgggacttt caggcggagc ggtctgttta
     4621 accggatgcg catggacctc gacgtgcagt tgggcgagac cgccctgcat ctgacgagcg
     4681 gcgagctgcc ggccgaaccg tggacccctg acatcgacat ggtcctgaag ggcaaggccg
     4741 tcgacgggac gaggcccggc acgtatgcgc tgcgtctggc tgccgtgcgc gagttggcgc
     4801 gctggcagta tcggttgatc gacggcagtt catggacgga cgactgcgag aagtgcgggc
     4861 ggcctacgat caggtggtcc ttgcacggcg gtttcgatct cgtcctggtc gagaaacatc
     4921 cactgttcaa ccgctatcat ctcttcgata ttcggttctt cagcggcacg gattatgaac
     4981 tcgaggggga aggcgattac caggtgggcg gcgaggttgc cttgcgtcag gaactgcggc
     5041 tggacgtgga agcgaaaggg acgttcggcg gaccgcgcac gatgaccctg accaatatgc
     5101 cgggtccgcc aacgcgcctt tggccgatgt gggccgtcga tgtgcaggag gctgagagca
     5161 cgccagagtc gaagctgctc cttgaactcc gggcggcccc cgtgcgcgaa ctttggtttt
     5221 cgacgacgca tggaatgaca gccggtctgc aacccggcct ccaaccccgc atatcgggcg
     5281 gcgatgtctt gaccgactcc ggccgccggg tgaagacaaa cgctcagttg atctccgccc
     5341 tgggatggac ggagccgaag gaagccctcg cggtggacgc cctgacgatc gctcccgggg
     5401 gcgaggtctg gttctcgttc agcgaggggc agacgagccc gacgctcggg gtgatcagtg
     5461 atggcgatct ggtgtcggac cacggacgca tcgtcagacg aaatggcgag ctgatggcgt
     5521 cattcggcct gatgccgccg ctgcctgatc tcgggctgga cggtgtccag gtattggatg
     5581 atggcgagat cctgttttcc atcgttcgcg agtcgttctc cgagcgtctg ggcgtcatgc
     5641 tgcgacccgg cgacatcctc tcgagtcgtg gaaacgtcac ccgcgcggcc aaggagttgc
     5701 tggctgtttt tgagcccgaa gatcaggccg ccgaccccgg actcgatgcg ttctttgttt
     5761 ggccgagcgg cgaggtgtgg ttctcgactg agtccgggtt tatcagcaag acgatgggac
     5821 cgatcatgga cggcgcgttg ctcagcgacc aaggctatgt cgtcgcgcac aacctcgatt
     5881 tggtccagcc ttttcaacct ctcgaagacc tggccaattt cggcctcgac ggcctgttcc
     5941 tcgtgacgga cgtcgcggcg ccggcaaaac cggctcgagt gctgctcagc ctcaagaatg
     6001 gagagggcgg cggagtccgg ctgacatggc ccgcgcaggg gcgcgtgttt caggtcgagg
     6061 cgacgaccgc tgtgggcgat ccgtttgccc ccgtcagccc aatcgttcca gacctcgaat
     6121 ggatggattc atccgcttca gggttgacgc agagctctta ttatcgcttg aggcagtggt
     6181 gaaatgtgcg cgatacccga tcagccgtac atccgaactg aacccagagt gacactcgtg
     6241 agctgccggc catcgcggcg tgcgcctgcc ggggctttgc ccgcggaagc gacgccgcga
     6301 ggccgatcgc tcatgacaga gccgatgctc aaatcagccg gctccgtcct gggatgtaca
     6361 cggggtaact gccgttcggg cccggtttgg tgggcggctc catgccgtcg cggcagtcct
     6421 cgggcctggg cgggaagaag aagtccgagg cggtgatctg ctcccaggtc acttctttac
     6481 cggtgtagca ggagatttgc cccatgatcc cgatgagcgt gctgggcgcc atgtaatcgc
     6541 cgttgttgat cggtgtgccg gcgcggatgg ccttcatgta ctcgacctgc tcctggtagt
     6601 gagcgtcgca accggcgtcg ccgcgccagt tcttctcgcc ccagatgcgg caggccatga
     6661 cgtcggcgcg gcccttggtg ccgaagatca ggctcgagta ctcgtcatag caaccggtgg
     6721 tcgtgcggca cagggcatag acccgcgcgc cgttttcgta ctgatagacc accgtgtggt
     6781 gatcgaacac gtcgccgtag atcgcatcgg tcatgctcga acgaccgccc aggccgtggc
     6841 acttgaccgg ggcctgttct tggagagccc aactggcgcg gtccatgttg tggaccagag
     6901 attgcacgac gtcgtcgccc gagagccaaa cgaagtggta ctgggtgctg aattgccatt
     6961 cgagttcgga aagccccgg
//
LOCUS       DAOQRO010000167         6959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10667_length_6959_cov_4.659038, whole genome shotgun sequence.
ACCESSION   DAOQRO010000167 DAOQRO010000000
VERSION     DAOQRO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10667_length_6959_cov_4.659038"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKK20_07505"
     CDS             complement(<1..443)
                     /locus_tag="PKK20_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNU99771.1"
                     /translation="MSFFDRRNEMMPRAQLEQLQLERLQALLVRLKRNVRRCREKLGE
                     ARVQSLDELERLPFTTPEDLAQCFPYGMFAFPLREIIRLHTAVGAEGKPLVIGHTRND
                     LLEWGRLAGRQLVAGGVTANDVVQIGLGPSNYPGLSGYVLGAELI"
     gene            complement(440..1156)
                     /locus_tag="PKK20_07510"
     CDS             complement(440..1156)
                     /locus_tag="PKK20_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99772.1"
                     /translation="MLQVKNLRCGYGSLEVVHGISLHVCAGEIVGLLGANGAGKTSLL
                     KSVVGLLQPWQGEIHVDGRPTRGQAAWRSISHSMVLVPEGKMIFADLTVRENLLIGGY
                     RNPDRAMQIEIVFDRFPLLRERASQLGGTLSGGEQQMLALGRALMARPKILLLDEPSL
                     GLAPLMVKEVFTEIVRMRDQGLTVLLVEQNVTATLQVADRAYVMETGDIILEGNAADL
                     MHNPEVKRAYLGKGAKEIWE"
     gene            complement(1143..1922)
                     /locus_tag="PKK20_07515"
     CDS             complement(1143..1922)
                     /locus_tag="PKK20_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99773.1"
                     /translation="MALLEIDNVRHSFGGLVAIDRVSFEVAPGQIKSVIGPNGAGKTT
                     LFNIVCGLLRPESGSIRFGDRSITGFKPYQIARAGVSRTFQNPSLFLQMNVLENVMVG
                     RHCRTRWEFLGCGVRWPGQPKEEAAIREAAQTQLEYVGLAHLAECPAGALAFGERRMV
                     ELARALATEPQLLLLDEPGSGLNTREKADLGRMIRRVRERGVTILLVEHDMTLVMDLS
                     DAILVLHNGARIAEGTPSEIQNDDKVVRVYLGGEMNHVAAS"
     gene            complement(2059..3210)
                     /locus_tag="PKK20_07520"
     CDS             complement(2059..3210)
                     /locus_tag="PKK20_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNU99774.1"
                     /translation="MKPRLKPGLSFTLLAAALIVVPWILRWADMEYCLTQLTMSLYYA
                     IVVLGLCLVMGYAGQVSLGHGAFFAIGGYTSAVLTTCDVSKWQTAAWLGWIKQSPFCM
                     AREDLFGNPILTLSPWVAFFAAMALAFVVANLIGYPALRLRGHYLAMATLGFGMIVNK
                     VILGTAFTGAADGINGVPEWNLGLGLTVSGKRALRIENYFIASGLLLLLLVLLHNLVR
                     SRVGRALQAIHDRETAANAMGINTALYKLKAFVLSALMAAMAGVFLTHYTAGIGPSEA
                     GALKSVRYVALAAAGGMSSLWGVTGMSTLLNYMSLRGLFGSYDNAVFGLLLIGIVSLA
                     PEGPLKPLARLLRRFVGACRPPNAVGPDAARPDNGPARRASDQTQCLFP"
     gene            complement(3207..4100)
                     /locus_tag="PKK20_07525"
     CDS             complement(3207..4100)
                     /locus_tag="PKK20_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNU99775.1"
                     /translation="MESGNLELQYFLSGITKGSIYAVVAIGFNLIYSATGVLNFAQGE
                     FVMLGGMVAVTLARFVPLPVALAGAVAIVALVGCLLEILFFRRLQRHSILHLVIITIG
                     LSIVLQEIALHLWDEKVRSLPYFTGNEISSLKFFGAAISPQVFWVLATVAVTVVLLHI
                     FLKYTLPGRAMRAGSSNPSAAMLAGINIANMRTLAFGLSAAIGALAGCVISPITMTHY
                     EMGAPLAIKGFAAAILGGLGNPMAAVLGGLMVGVLEAYSVSRLPAAYSDVTVFAVLLL
                     VLFARPHGFWGRPGGESVREH"
     gene            complement(4181..5344)
                     /locus_tag="PKK20_07530"
     CDS             complement(4181..5344)
                     /locus_tag="PKK20_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNU99776.1"
                     /translation="MKRTYLLVAGICALGAQCLTQNIHAAAGGDSIKIGAIFSVTGPA
                     SFLGAPEEKTVRLFVDKLNAAGGIHGRRVELVLKDSGGSPEKAVSFAKQLIEEEKVLA
                     IIGPSTSGETMQIKALCEENEMILVSCAAAEVIVNPTAKYVFKTPQKDSQAVTWIYRT
                     MKDKGVRKIAVVSSNDGFGAAGKKQLEDMAPAEGIEILANEVYDKQATDLTDILTKIK
                     GTRGVQAIVNWSIVPAQSIVAKNMRQLGMEMPLFQSHGFGNRKYVEQAGVAAEGTLFP
                     AGRLLVVDELPDNHPQKALLAAYKKDYEETYKEDVSTFGGHAYDAILVVTEALKRARR
                     PDRARVRDAIENLKGLAGTAGVFNFSPTDHTGLDLTSFEMLTVKDGKFAIYRK"
     gene            5698..>6959
                     /locus_tag="PKK20_07535"
     CDS             5698..>6959
                     /locus_tag="PKK20_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99777.1"
                     /translation="MAIVIQGPGVEQAARTTLSGHAWPATLFLDDRVLVTAEPPVHAL
                     QRAAYLNALNLERAQQGLAPLTEKQKAKRCRRSVDLFLKPGLVQIRPDPGHMDLAFEA
                     DDLLGDLYPKPQRRFLDVDQPEVQRAIKERGELWRISVLPSTRAEISQHIADAKTRIS
                     EQAIYYLNLYSGTRYLTCQEFAQLKNLDPPALARQLQEIADYASRRNRLGRLEVDFFK
                     AQGRFGAKQFNGLVFGELNPNELAACHEQLRVQFFDAVPPDYQVDEPDLDLWRTEMYS
                     ALVSQRDDTETEVVLAGLNIEFKQRIRWLPCGRFVQGELMFDEVFESARQAPENPCYR
                     DLCDEKVKDIVFNLIREFGLLEWVNVGRILPLHQETQHGAQARPVSGGRRDVYLAQIK
                     CVTEDRPRTRFMRMQKYGVRERLAEGKD"
BASE COUNT         1301 a         2143 c         2259 g         1256 t
ORIGIN      
        1 tcgatgagtt cggcgcccag aacgtaaccc gagagtccgg ggtaattgct cgggcccagg
       61 ccgatctgca ccacgtcatt ggccgtgacg ccgccggcga caagctggcg cccggccagc
      121 cgcccccact cgaggagatc gttgcgggtg tggccaatga ccagcggttt gccttcggcc
      181 ccgaccgcgg tgtgaaggcg aatgatctcg cgcagcggga aggcgaacat cccatacgga
      241 aagcactgcg ccagatcctc gggcgtcgtg aacgggaggc gttcgagttc gtcaagggat
      301 tggacccggg cctcgccgag cttttcgcgg cagcggcgca cattgcgttt gaggcgcacc
      361 agcaatgcct ggaggcgttc gagctgcaat tgctcgagtt gggcgcgggg catcatttca
      421 ttgcgtcgat cgaaaaagct cattcccaga tctccttggc gcctttgccc aagtaagcgc
      481 gtttgacttc gggattgtgc atcaggtcag ccgcgtttcc ctcgaggatg atgtcgccgg
      541 tctccatgac ataggcccga tcggcaacct ggagggtagc cgtcacattc tgctcgacaa
      601 gcagcaccgt caaaccctgg tcccgcatcc ggacaatctc ggtaaagacc tccttgacca
      661 tgaggggcgc gagcccgagc gacggttcat cgagcaggag gattttgggg cgggccatga
      721 gcgcgcggcc cagggcgagc atctgttgct cgccgccgga caaggtgccg cccaattggc
      781 tggcccgctc gcgcagcaac gggaaccggt cgaagacgat ctcgatctgc atggcgcggt
      841 cgggattgcg gtagccgccg atgagcaggt tctcgcgcac ggtcaggtcg gcgaagatca
      901 tcttgccttc gggcacgagg accatcgagt ggctgatgct gcgccaggca gcctggccgc
      961 gcgtggggcg gccatccaca tgaatttcgc cctgccatgg ctggagcagg ccgacaacgg
     1021 atttgagcaa agaagtcttg ccggcgccgt tggcgccgag gaggccgacg atctcccccg
     1081 cgcagacgtg caggctgatg ccatgcacga cttcgaggct gccgtagccg cagcggaggt
     1141 ttttaacttg cagcaacatg gttcatttct ccgcccagat agaccctcac gaccttgtcg
     1201 tcgttctgga tctcggaggg cgtgccttcc gcgatgcggg cgccgttgtg cagcaccaga
     1261 atggcatccg acaaatccat caccaaggtc atgtcgtgtt ccacaagcag aatcgtgacc
     1321 ccccgctcgc ggacccggcg gatcatccgg cccaggtcgg ccttttcgcg tgtgttcaac
     1381 ccgctgcccg gttcgtccag gagcaggagt tgcggctcgg tggcgagggc gcgggccagt
     1441 tcgaccatcc gacgttcccc gaaggccagc gctcctgccg ggcactcggc caggtgcgcc
     1501 aggccgacgt attcgagttg tgtctgcgcg gcctcacgga tggcggcttc ctccttcggc
     1561 tggccgggcc agcgaacgcc gcagccgagg aactcccagc gtgtccggca gtgccgtccc
     1621 accatgacat tctcgagcac gttcatttgc agaaagaggc ttggattctg aaacgtgcgc
     1681 gacacgccgg cgcgggcgat ttggtaaggt ttgaatccgg tgatggatcg gtccccgaac
     1741 cggatgcttc cggactccgg gcgcagaagt ccgcagacaa tgttgaacaa cgtcgtcttg
     1801 cccgctccgt tcggcccgat gaccgacttg atctgtcccg gcgcgacttc gaacgaaacg
     1861 cggtcgattg cgacgaggcc gccgaagctg tggcggacgt tatcgatttc aagcagcgcc
     1921 atggcgcggc ctcctctcta gaatccatga ccggtgaaac atgttgctga atagtcaggt
     1981 tcctgtgctg cgttcaaggg gtcgaggatt ccagcgagcg aggcgcggga cccagaggcg
     2041 gggaaacacg aggacttgtc atggaaataa gcactgggtt tggtctgagg cgcgcctcgc
     2101 cggcccgttg tctggtcgcg ccgcatcggg cccgacggcg ttgggtggac ggcatgcgcc
     2161 gacgaagcgc cggagcaggc gcgccagcgg tttgagcggt ccttccgggg cgagcgacac
     2221 gatgccgatc agcagcaggc cgaacaccgc gttgtcgtag cttccgaaga ggccgcgcag
     2281 cgacatgtag ttcagcaggg tgctcatgcc ggtgacgccc cagagactgg acatgccgcc
     2341 cgccgcggcc aacgccacat aacggaccga cttgagggcg cctgcctccg aggggccgat
     2401 tccggcggtg taatgggtga ggaacacgcc ggccatcgcg gccatcagcg cgctgaggac
     2461 gaacgccttg agcttgtaga gcgcggtgtt aatccccatc gcgttggcgg cggtttcgcg
     2521 atcgtggatg gcctggaggg cgcgtccgac acgggagcgg acgaggttgt gcaggaggac
     2581 gagcagcagc agcaacaacc cgcttgcgat gaagtaattc tcgatgcgca gggcgcgttt
     2641 gcccgagacg gtcaagccca ggcccaggtt ccactccggc accccgttga tgccgtcggc
     2701 cgccccggtg aaagcggtgc cgaggatcac cttgttgacg atcatgccga agccgagtgt
     2761 ggccatggcg aggtaatgcc ctcgaagccg aagcgcgggg tagccgatca ggttggcgac
     2821 gacgaaggcg agggccatcg ccgcgaagaa cgccacccag ggcgacagag tcagaatcgg
     2881 attgccgaac aggtcctctc tggccatgca gaaaggcgac tgtttgatcc agccgagcca
     2941 tgccgccgtt tgccatttcg agacgtcgca cgtggtcagc acggccgagg tgtagccacc
     3001 gatcgcaaaa aacgccccat gccccagcga gacctggccg gcgtagccca tgaccagaca
     3061 gagccccaaa acgacgatgg cgtagtagag cgacatcgtg agttgtgtca ggcaatactc
     3121 catatccgcc cagcgcagaa tccagggcac gacgatcagg gccgccgcga gcagggtaaa
     3181 ggagaggcca ggcttgagtc tcggcttcaa tgttccctca cgctttcccc gcccggtcgg
     3241 ccccagaacc catgcggacg cgcgaacaag accagcagca acacggcgaa aacggtcacg
     3301 tcgctgtacg cggccgggag ccggcttacg ctgtaggcct cgagcacacc gaccatcagc
     3361 ccgccaagca ccgcggccat cgggtttccc agaccgccga ggatggcggc tgcgaatccc
     3421 ttgatggcca gcggcgcgcc catctcgtag tgcgtcatgg tgatgggcga gatcacgcag
     3481 cccgccaggg cgccgatggc cgcgctcaga ccaaacgcga gcgtgcgcat gtttgcgatg
     3541 ttgatcccgg ccagcattgc ggcgctcggg ttcgaggaac cggctcgcat cgcccggccc
     3601 ggcaaggtgt atttgagaaa gatgtgcagc aggaccaccg tgaccgccac ggtcgccagg
     3661 acccagaaca cctgcggcga gatggcggcc ccgaagaatt tcaacgaaga gatctcgttg
     3721 ccggtgaaat aggggagcga ccggactttt tcgtcccata ggtgcagcgc gatttcctga
     3781 aggacgatcg acaggccgat ggtaatgatg acgaggtgga ggatggaatg ccgctgcaaa
     3841 cgccggaaga ataggatctc gagcaggcag ccgaccagcg ccacgatggc caccgccccg
     3901 gccaatgcca cgggcagcgg gacgaagcgg gccagggtca cggccaccat tccgccaagc
     3961 atcacaaact cgccctgcgc gaagttcaac acgccggtgg cgctgtagat caggttaaag
     4021 ccgatcgcca ccacggcgta gatgctgccc ttggtgattc cggacaggaa gtattgcagt
     4081 tccagattcc cgctttccat cgatcgtccc gtcgggagac agccccgatc caatgcgccg
     4141 gcggggctcc ggaagtgagg cttgttgttc tcgagggagg ctatttccgg tagatcgcga
     4201 acttgccgtc cttcaccgtc agcatctcga acgaggtcag atcgagaccg gtgtgatcgg
     4261 tgggtgaaaa attgaacacc cccgccgtgc cggccagccc tttgaggttc tcgatggcgt
     4321 cacggaccct ggcgcggtcg ggccgtctcg cccgcttcag ggcctcggtc acaaccagga
     4381 tcgcgtcata ggcgtgtccg ccaaacgtgc tgacatcttc cttgtaggtc tcctcgtagt
     4441 ccttcttgta ggcggccagc agcgccttct gcgggtgatt gtcgggcaat tcgtccacaa
     4501 cgagaaggcg cccggccggg aagagcgtgc cctcggccgc gacgcccgcc tgctcgacat
     4561 acttgcggtt gccgaagcca tgactttgga agagcggcat ctccatgccg agctgccgca
     4621 tgttcttcgc cacgatggac tgcgccggga cgattgacca gttgacgatc gcctgaacgc
     4681 cgcgcgtgcc tttgatcttt gtgaggatgt cggtcaggtc ggtggcctgt ttgtcgtaga
     4741 cctcgttggc gagaatctcg atgccctccg ccggggccat atcctcgagt tgcttttttc
     4801 cggcggcgcc aaagccgtcg ttgctgctca cgacggcgat cttgcgcacg cccttgtcct
     4861 tcatggtccg atagatccag gtgacggcct ggctgtcctt ttgcggtgtc ttgaacacat
     4921 acttggccgt cggattcacg atcacctccg ccgcggcgca cgagaccagg atcatctcgt
     4981 tctcttcgca gagcgccttg atctgcatgg tttcgccgct ggtcgacggg ccgatgatgg
     5041 ccagcacctt ctcctcctcg atcaactgtt tggcgaacga cacggctttc tccgggctgc
     5101 cgccactgtc cttgagcacc aactcgactc tgcgtccgtg gatgccgccg gccgcgttga
     5161 gcttgtccac aaacaaccgg acggtcttct cctccggtgc gcccaggaaa gaggccggcc
     5221 cggtgactga gaagatggcg ccgatcttga tggagtcgcc gccggcggcg gcgtggatgt
     5281 tctgggtcag gcactgcgcg ccgagcgcgc agattcctgc cacgagcagg taggttcttt
     5341 tcataagctc attctccgga ttcaaaccac tgtgaaccga gcatcactga aacaaggcac
     5401 caaaacgggt taagttgagt cgaggctgcc agaaaggctt tccgcatgtc aaactccatt
     5461 cccaccgaca accgacagat catcgccaat tcatgaagtg gagcggcagg cagggcaggc
     5521 agcgccccgt cgcagggaaa cgccgtcttc acttgacatg gcgggggacg agccgttccc
     5581 cactccggcc ttttcgtctt catgagttgg ccctgccgac ggatgcgctg tccggatgca
     5641 ctccgctcac agcggggtgg acaaaccgac gcgggtgcgc atgatggcgt ccggattatg
     5701 gctatcgtca tccagggacc gggggtggag caggccgcca ggacgacgct gtcgggtcat
     5761 gcgtggcccg ccactctgtt cctcgacgat cgcgtcctgg tgaccgcgga accgccggtg
     5821 cacgccctgc aacgcgcggc ctatctcaac gcgctcaacc tcgagcgggc gcagcagggt
     5881 ctggcgccac tgacggagaa gcagaaggcc aagcgctgcc ggcggtctgt cgatttgttc
     5941 ctgaagccgg gtctggtcca gattcggccc gacccaggcc acatggattt ggcgttcgag
     6001 gcggacgatc tgcttgggga cctctatccg aagcctcaac ggcggtttct ggatgtcgat
     6061 cagccggagg tgcagcgggc catcaaagag cggggcgagc tatggcggat cagcgtcctg
     6121 ccgagcaccc gcgccgaaat cagccagcac attgccgacg ccaagacccg catcagcgag
     6181 caggccatct attacctcaa cctgtatagc ggcacacgct atctgacctg ccaggagttt
     6241 gcgcagctca agaatcttga cccacccgcg ctcgcgcgcc agttgcagga gattgccgac
     6301 tacgcgtctc gccgcaaccg tctgggccgg ctggaggtgg attttttcaa agcccagggg
     6361 cgctttgggg cgaagcaatt caacgggctc gtgtttggcg agctgaatcc gaacgaactt
     6421 gcggcatgcc acgaacaatt gagggtccag ttcttcgatg ccgtgccgcc ggactaccag
     6481 gtcgatgagc cggatttgga tttgtggcgg acggagatgt actcggcact ggtgagccag
     6541 cgggacgaca ccgagacgga ggtggttttg gcgggtctga acatcgagtt caagcagcgc
     6601 attcggtggc tgccgtgcgg gcgtttcgtc cagggggagt tgatgtttga cgaggtgttc
     6661 gagagcgcgc ggcaggcgcc ggagaatccg tgctaccgcg acctgtgcga tgagaaggtc
     6721 aaagacatcg tgttcaacct gattcgtgag ttcggcctgc tcgaatgggt gaacgtggga
     6781 cgcatcctgc ccctgcatca agagacgcaa cacggcgccc aggcccgacc cgtcagtggc
     6841 ggacggcgcg acgtgtatct ggcccagatc aagtgtgtga ccgaggaccg gccccggact
     6901 cggttcatgc ggatgcagaa gtacggggtg cgggagcgat tggccgaggg gaaggactt
//
LOCUS       DAOQRO010000168         6941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10711_length_6941_cov_4.571595, whole genome shotgun sequence.
ACCESSION   DAOQRO010000168 DAOQRO010000000
VERSION     DAOQRO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10711_length_6941_cov_4.571595"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKK20_07540"
     CDS             complement(<1..366)
                     /locus_tag="PKK20_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99778.1"
                     /translation="MKALYALCLVLVAPVLASKADFQAGVARISITPPMPFWLTGYAA
                     RTNAAASVRTELWAKALALEDSRGGRAVLVTSDVIGLPREISDAVAERAALLDRAREP
                     EQRLAALVRLQDRGQPDVAA"
     gene            735..989
                     /locus_tag="PKK20_07545"
     CDS             735..989
                     /locus_tag="PKK20_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNU99779.1"
                     /translation="MPTYDYECGHCGHRFERRQRMSDPPLQTCPACGKEVRRQFGAGA
                     GVILKGGRKSHGSAAPCAWESTGQTCCGRSSRCNSSSCEF"
     gene            1193..2515
                     /locus_tag="PKK20_07550"
     CDS             1193..2515
                     /locus_tag="PKK20_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNU99780.1"
                     /translation="MASVVLCATAVHAQETPAQKAERMAWFNNDRFGLFIHWGVYSVP
                     AGEWQGKTNYGEWFLEETKMPVSQYMQYAKEFNPVRFDARQWVRMAKDAGMKYIVITS
                     KHHDGFGIFRSNLTDWCMKATPFPRDPLAELAAACRVEGLRLCFYHSIMDWHHPDWET
                     RRAWNDTAPATPPDMERYIRFMKGQLKELITRYGPLGILWFDGEWEKPWTVERGRDLY
                     NYVRSLQPDIIINNRVGKGRAGMDGMDKGAGGVGDYGTPEQEIPATGFGPGVYWESCM
                     TMNNHWGYNKHDQNWKSTRTLVRNLIDCASKGGNYLLNVGPTSEGLFPEPSVQRLAEI
                     GRWMKVHHEAIYGTSASPFKHLPWGRCTQKAAPDSTTRLYLHVFDWPADGNLLVPGLK
                     NSVDSAALLGSDATVRFAQTPEGVALTLPRVAPDALSSTVLLKIQGKP"
     gene            2525..2947
                     /locus_tag="PKK20_07555"
     CDS             2525..2947
                     /locus_tag="PKK20_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99781.1"
                     /translation="MNQLQTPRHTATRTFLRVAGLLLGSTGLVFLIIGLSSFFAAFGG
                     SGSPRLFWCCWVGIPLLFVGGVMCLFGFMGAVARYTAAEQVPVAAAAISDLASGTQDA
                     VKTVSRAVAEGVLEAHSDKKSCGCSKNGVNQDTPDRQH"
     gene            complement(2970..4115)
                     /locus_tag="PKK20_07560"
     CDS             complement(2970..4115)
                     /locus_tag="PKK20_07560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU99782.1"
                     /translation="MLNWRDPNNPLAGGAERVTAGYLSALRQRGHEVFWFANLFSGAA
                     IEETVEGIPVVRGGGKGSSVLAARRWYRRQRPFDLVIDQHHGIPWYAPWWSGTRCVAY
                     IHEVLGPIWDVFYNWPWSAIGKWQERRTIWLYRNVQFWTASASTKTILSEIGVRHVQL
                     IPYGVHTTAIPELESKPIEQPVRLVMVSRLAPNKRVDHGIRALRCLADRGIRATLNIV
                     GAGDMEPALRALTSNLRVGGMVRFSGPLSEAAKDAALREAHLLLHASLREGWGLNIIE
                     ANAMGTPAVVYPVAGLIEATVQDQTGRVADSECPEALADAVQRCVEAQEDYQRYRRCA
                     WERAKTFHWSQVLPKACDWLEARASSQSSPLAKSDRPVHRRGANHAG"
     gene            4233..>6941
                     /locus_tag="PKK20_07565"
     CDS             4233..>6941
                     /locus_tag="PKK20_07565"
                     /inference="COORDINATES: protein motif:HMM:NF028477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5127 domain-containing protein"
                     /protein_id="HNU99783.1"
                     /translation="MMKLATVFLLTLGALAGLITPHAADWQPAQGPLMTRWAKDVSPQ
                     NALPEYPRPQLKRADWLNLNGLWDYAIADKDASRPAAWDGRILVPYPLESALSGVMKR
                     LGENQRLWYRRTFQVPDAWIGKQVLLHFGAVDWEATVWVNRQEVGRHRGGYDAFKFDM
                     TTALKPGAEQEIVVAVWDPTDAGYQPRGKQIRNPHGIWYTPTSGIWQTVWIEPVAATH
                     LDAVRITPDVDAGAVRLDFKLVGGTGDVSVKAEIQDGTQIVASGAIGEKNRAAAGPFS
                     TILELELRDARLWTPDTPFLYGLKLTVSHNGTTTDQAESYFGMRKSALVKDANGTLRL
                     GLNNKPLFQFGPLDQGFWPDGLYTAPTDEALRYDIEMTKRLGFNMARKHVKIEPARWY
                     YWCDRLGLLVWQDMPSGDKYIGGSDPDITRSPESARNFETELKALVDGFYNHPSIVMW
                     VPYNEGWGQWDTPRIVDLIRSWDPTRLVNNASGWTDRKVGDVMDIHSYPGPAAPRIEE
                     MRAGVLGEFGGLGLPLQGHTWQDEKNWGYRSYKNSDDLTDAYIALIRRLRPLTGEPGL
                     AAAVYTQTTDVEIEVNGLMTYDRAVVKLDEAAIRAANLTVYTPPPPRRAEGATLVPPA
                     TPLVACDPYFSIWSPADRLTDEDTTHWTGKPHRLTSMIRIDGKPYRVMGPSPVGVPAL
                     DQVDLAVLPTRTAYRFQGDGVALALMFLTPVLPHNIDVLSRPVTYLIWDVKSLDGQKH
                     DVAIYFDASSELTVNDPAQAVVWSKEAFGPLVALKVGSKDQPVLAKKGDDLRIDWGYL
                     YAAAPAAAVADARVAPRDEWAARFARGERLAAISAAAPAEGRGDFFTAAIAFDLGWVA
                     AAPVSRWLMLAYDDLWSIQYMHNNLRPYWRRNGWEAVAAHRRRCPRR"
BASE COUNT         1355 a         2333 c         2069 g         1184 t
ORIGIN      
        1 cgccgcgacg tcgggctggc cccgatcctg caggcgcacc aacgccgcga ggcgttgctc
       61 cggctcgcgc gcgcgatcga gcagggccgc ccgctccgca acggcatcgc tgatttcacg
      121 cggcaggcca atcacatcgc tcgttacgag gaccgcccgg cctccgcggg aatcctcgag
      181 agccagggcc ttcgcccaga gttccgtccg caccgacgcc gcggcattgg tgcgtgcagc
      241 gtaacccgtc agccagaacg gcatgggagg cgtgatggaa atgcgggcca cacccgcttg
      301 gaaatcggct ttggaggcca gcacaggagc aaccaaaacc aggcacaagg cgtaaagggc
      361 tttcatctga aatagagagg accatcccag gtttcccctg aaagtcgagc ccatttcatg
      421 ccctgtccca acacacaacc cgcccctgcc tcccatgaat cgacgagttg agagttgaga
      481 gttggacgtt gaacgtttcg gtttcatcgc ggcaagtcac attaagtcgt tggacgtcaa
      541 aacgctccat gaaccgagcg ccaaccgcgt gcgccagcgc agtcttcccg gcaccgattc
      601 gccatctgcg caggcccgga aatggcccaa aaatggcgtg ctttgggcaa ccgttctggt
      661 tgaatggcag cctcgattgg acgaagccgg acgcgaagac acaatcacaa aatagacgag
      721 aatggaggaa gagaatgccg acgtatgact atgaatgcgg ccattgcggc caccgcttcg
      781 agcgaagaca acggatgtcc gatccgcccc tgcaaacatg ccctgcatgc ggcaaagagg
      841 tccgccggca gttcggtgcc ggcgccggtg tcatcctcaa gggcggcagg aaatcgcacg
      901 gctcagcagc gccctgcgca tgggaatcca ccggacagac ctgttgcgga cgcagttcac
      961 gctgcaattc ttcaagctgc gagttctgag gacgggctcg agcgccgttt gccaacacga
     1021 ccgcccggcg tgctccccgc cggaatccag tccccgtccg cgcatggaat tttctcgcgg
     1081 cctgagccgg gagccggtag tctgcgccca tcgtcacaat gcccgttgag actccaaatc
     1141 tcggttctgc cgagcaactg aaagagattc catgacacga tggctctgcg cgatggcttc
     1201 tgttgttttg tgtgcgaccg cggtgcacgc ccaggaaacg cccgcccaaa aggccgagcg
     1261 gatggcctgg ttcaacaacg atcggttcgg tctgttcatc cactggggcg tctattcggt
     1321 gcctgccggc gaatggcagg gcaagaccaa ctacggcgag tggttcctcg aggagacgaa
     1381 aatgccggtc tcccaataca tgcagtacgc gaaggagttc aatccggtca gattcgacgc
     1441 ccgtcagtgg gtgcgcatgg ccaaggatgc ggggatgaag tacattgtga tcacatcgaa
     1501 acaccacgac ggtttcggga ttttccgttc gaatctcacc gactggtgca tgaaagcgac
     1561 acccttcccg cgcgacccgc tcgccgagtt ggcggcggcc tgccgggtcg agggcctgcg
     1621 cctctgcttc taccactcga tcatggactg gcatcacccg gactgggaaa cccgtcgcgc
     1681 ctggaacgat acggcgccgg caacgccgcc cgatatggag cgttacattc ggttcatgaa
     1741 aggccaactc aaggaactca tcacccgtta cggtccgctg ggaatcctct ggttcgacgg
     1801 cgaatgggag aaaccgtgga ccgtcgagcg cgggcgcgac ctctacaact acgtccgcag
     1861 cctccaaccc gacatcatca tcaacaatcg cgtcggcaag ggccgcgccg gcatggacgg
     1921 aatggacaaa ggcgcgggcg gcgtcggcga ctatggcacg cccgaacagg aaatccccgc
     1981 taccgggttt ggtccgggcg tctactggga atcgtgcatg acgatgaaca accactgggg
     2041 ctacaacaag catgaccaga attggaagag cacaaggacg ctggtgcgca acctcatcga
     2101 ctgcgccagc aaaggcggca actacctgct caatgtgggc ccaaccagcg aaggcctctt
     2161 tccagaaccg agcgttcagc gtttggcgga gatcggacgt tggatgaaag tccatcacga
     2221 ggccatctac ggcaccagcg ccagcccttt caagcatctg ccttggggcc ggtgtaccca
     2281 gaaagccgca ccggacagca cgactcgact ttacttgcac gtgttcgact ggcccgccga
     2341 cggaaacctg ctcgtgccag gtctgaagaa cagcgtcgat tccgccgcct tgctcggaag
     2401 cgacgccacc gtgcgctttg cgcagacacc agaaggcgtc gcactgacgc tgcctcgagt
     2461 cgcgcccgac gccttgtcct ccacggtcct gctcaagatt cagggcaagc cctgaccgcc
     2521 ctctatgaac caactgcaaa caccgcggca cacggcaacc cgcacattcc tgcgtgtcgc
     2581 gggcctgctg ctcgggtcga caggtctcgt cttcctcatc atcggcctct ccagtttctt
     2641 cgcggccttt ggggggagtg gatcgccccg tctcttttgg tgttgctggg ttgggatacc
     2701 gctgctcttt gtcggcggtg tcatgtgcct gttcgggttt atgggcgcag tcgcccgcta
     2761 caccgccgcg gaacaggtgc ccgtcgctgc cgccgccatt tccgatctgg cctcgggaac
     2821 tcaggatgct gtcaagaccg tcagccgcgc tgtagccgag ggtgtgctcg aagcgcacag
     2881 cgacaagaag tcgtgcggct gttccaagaa cggagtgaat caagacacgc ctgaccggca
     2941 acactgagcg atcccgcggt tctcagcccc tagcccgcat ggttcgcacc ccgacggtgc
     3001 acagggcggt cagacttcgc aagaggactg ctctgcgagc tagcccgtgc ctcgagccaa
     3061 tcgcatgcct ttggcagcac ttggctccag tgaaaggtct tagcccgctc ccacgcacaa
     3121 cgtcgatacc gctgataatc ctcctgcgct tcgacgcacc gctgcacggc atccgcgagt
     3181 gcctccgggc attccgagtc cgcaacgcgc cccgtctgat cctgcaccgt ggcctcgatc
     3241 agcccggcga cgggatagac gacggcgggt gtgcccatgg cgttggcttc gatgatattc
     3301 agcccccacc cctcgcgcag ggaggcgtgc aggagcaaat gagcctctcg cagcgcagca
     3361 tccttcgccg cttccgagag cggccccgaa aaacgcacca tcccgcccac tctcagattc
     3421 gaggtcagag cccgcaacgc cggttccatg tcgcccgcgc ccacgatatt cagcgtcgct
     3481 cggatgcccc ggtcggccag gcatcgcaaa gcccgaatgc cgtggtcgac tcgtttgttc
     3541 ggcgcgagcc gtgacaccat gaccaggcgc acaggttgct cgatcggttt cgactcgagt
     3601 tccgggatgg ctgtcgtatg gacgccgtag ggaatgagct gaacgtgtcg gacgccgatc
     3661 tcggacaaaa tggtctttgt cgaggccgag gccgtccaga attgcacatt ccggtagagc
     3721 cagatcgtcc gacgttcctg ccacttgcca atggccgacc acggccagtt gtagaacacg
     3781 tcccagatcg gccccaacac ctcgtggatg tacgccacac accgcgtgcc cgaccaccac
     3841 ggcgcgtacc acggaatccc gtgatgctgg tcaatcacca gatcaaacgg acgctgccgc
     3901 cgataccatc gcctggccgc cagaaccgaa ctccccttgc cgcctccccg cacgaccggg
     3961 atgccttcga cggtttcctc gatcgccgcg ccgctaaaaa ggttcgcaaa ccagaacacc
     4021 tcgtgcccgc gctggcgtaa ggcagacaaa taaccggcag tcacccgctc cgcacccccc
     4081 gccagcgggt tgttcggatc tcgccaattc aacatcagaa accgcatcgg cgcgacacgg
     4141 tgcgccagaa agccgttccc cggcaagaac aactctgcga ggacagcgga tttgcgctcg
     4201 acgaacgccc tcgccaaagt gaaactgggc cgatgatgaa gctcgccact gtgtttctgc
     4261 tcacactcgg tgccctggcc ggcctgatca caccgcacgc cgcggattgg cagccggcgc
     4321 agggcccgtt gatgacgcgt tgggccaagg atgtgtcgcc ccaaaacgcc ttgccggaat
     4381 acccgcgtcc gcagttgaag cgcgcggatt ggctcaatct caacggcctc tgggactatg
     4441 cgattgccga caaggacgca tcccgacccg ccgcatggga tggccggatt ctcgtcccct
     4501 atccgctcga atcggcgctg tcgggcgtca tgaagcgcct gggcgagaat cagcggctct
     4561 ggtatcgccg gacgtttcag gtgcccgacg cctggatagg caagcaggtg ctgctccact
     4621 ttggcgccgt cgattgggaa gcaaccgtgt gggtcaatcg ccaggaagtg gggcgccatc
     4681 gcggcggata cgacgcgttc aagttcgaca tgaccaccgc cctcaaaccg ggcgcggagc
     4741 aggaaatcgt cgttgcggtc tgggacccga ccgacgccgg ttaccagccg cgcggcaaac
     4801 agattcgcaa tccgcacggc atctggtaca cgcccacaag cggcatctgg cagacggtct
     4861 ggatcgagcc ggtggccgcc acccatctcg atgcggtccg gatcactccc gatgtcgatg
     4921 ccggcgcggt ccggcttgac ttcaagctgg tgggcggcac gggcgacgtg tcggtgaagg
     4981 cggaaatcca ggatggcacc cagatcgtcg ccagtggcgc gatcggagaa aagaaccgcg
     5041 ccgccgcggg cccattctcg acgattctcg aactcgagct cagagacgcc cggctctgga
     5101 caccggacac gccctttctc tacgggctca agctcaccgt atcgcacaac ggcaccacga
     5161 ccgaccaggc cgagtcctac tttggaatgc gcaagagcgc gctggtcaag gacgccaacg
     5221 gcacgcttcg actcggtctc aacaacaagc cgttgttcca gttcggcccg ctcgatcagg
     5281 gattctggcc cgatggattg tacaccgcgc cgacggacga ggcgctccgc tacgacatcg
     5341 agatgacgaa acggctcggc ttcaacatgg cccgcaaaca cgtcaagatc gagccggcgc
     5401 gctggtacta ctggtgcgat cgactcggcc tcctcgtctg gcaggacatg cccagcggcg
     5461 acaagtatat cgggggatcg gatcccgaca tcacccgctc gcccgagtcg gctcgcaact
     5521 tcgaaaccga actcaaggcg ctcgtcgacg gattctacaa ccacccgagc atcgtcatgt
     5581 gggtgcctta caacgaaggc tggggccaat gggacacacc ccgcatcgtc gatctgatca
     5641 ggtcctggga tccaacccgt ctcgtcaaca acgccagcgg ctggacggat cgcaaggtcg
     5701 gcgacgtcat ggacatccac agctaccccg gcccggccgc accgcgaatc gaggagatgc
     5761 gcgccggtgt cctgggcgag tttggcggcc tgggcctgcc gctccagggc cacacgtggc
     5821 aagacgaaaa gaactggggt taccgcagct acaaaaactc cgacgatctc accgacgcct
     5881 acatcgctct catccgcaga ctccgtcctc tgaccggcga gccgggtctc gccgccgccg
     5941 tctatacaca gaccaccgac gtcgagatcg aggtcaacgg cctcatgact tatgaccgcg
     6001 ccgtcgtcaa gctggacgaa gccgccatcc gcgccgcgaa cctgacggtc tacaccccgc
     6061 cgccgccgcg ccgcgccgaa ggcgccaccc tggtcccgcc agccacgccg ctggtcgctt
     6121 gcgaccctta tttcagcatc tggtctcccg ccgatcggct gacggatgag gatacgactc
     6181 actggaccgg caagccgcat cggctcacca gcatgatccg gatcgatggc aagccctacc
     6241 gggtcatggg cccaagcccc gtcggcgtgc ctgcactcga tcaggtcgac ctggcggtcc
     6301 tgccgacacg caccgcctac agattccagg gcgacggcgt cgcgcttgcg ctgatgttcc
     6361 ttacccccgt cctgccgcac aacatcgacg tgctttcacg tccggtcacc tacctgatct
     6421 gggacgtcaa atcgctcgat ggccaaaagc atgacgtcgc aatctacttc gacgcctcct
     6481 ccgaattgac ggtcaatgac ccggcacaag ccgtggtgtg gtcaaaggag gcgttcggtc
     6541 ctctcgtcgc gctcaaagtc ggctcgaagg accagcccgt cctggccaag aaaggcgacg
     6601 atctgcgcat tgactggggt tacctgtacg ccgcggcgcc cgccgccgcg gtggccgatg
     6661 ccagggtggc gccgcgggac gagtgggcgg cgcgtttcgc gcggggcgaa cgtctcgcgg
     6721 cgatctcggc cgccgcgccg gccgagggac gaggcgattt cttcacggcc gcgatcgcct
     6781 tcgacctggg ctgggtggcg gcggcgcccg tctcccgctg gctcatgctg gcctatgacg
     6841 acctctggtc gattcagtac atgcacaaca acctgcggcc ttactggcgc cgcaacggct
     6901 gggaggccgt cgccgcgcat cgtcgccggt gtccacgtcg c
//
LOCUS       DAOQRO010000169         6886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_10840_length_6886_cov_3.695506, whole genome shotgun sequence.
ACCESSION   DAOQRO010000169 DAOQRO010000000
VERSION     DAOQRO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10840_length_6886_cov_3.695506"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKK20_07570"
     CDS             complement(<1..214)
                     /locus_tag="PKK20_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNU99784.1"
                     /translation="MSTFDSSYRLIAPDVILGNGVRLYGFTNLYGCEIGDDSKIGTFV
                     EVQKGAKIGRRCKVSRHTFICEGVIIE"
     gene            complement(235..1377)
                     /locus_tag="PKK20_07575"
     CDS             complement(235..1377)
                     /locus_tag="PKK20_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99785.1"
                     /translation="MPTPIRVGVVGCGYWGPNLMRNLRSLSNCRLEVICDVKQERLKH
                     LGSLYPEARTMTDYNAMVTRKDLDAIVIATPVEFHHRMAKASLSAGKHTFIEKPMAAS
                     SAECEDLIHLADGKGLVLMAGHTFLYSPAVRKIKEIVDAGDIGDIRYISARRLNLGLY
                     QRDINVTWDLAPHDISIILHIMEESPLSVNCRGSAHVTPGIEDVTSLFLTFRKERSAI
                     IHSSWLDPRRVREMTIVGSRRMIVYDDVAQLEKIRIFDARVERPPHYDTFAEFHYAYH
                     YGDVYAPYIKQEEPLKVECQHFLDCIRNGTRPLTDGRQALAMVRILEAASASLKQEGG
                     PVHFDKEADHAPATSMVKSTAPRAHAASKRNGRPPRKEVAVSLAAA"
     gene            complement(1428..2117)
                     /locus_tag="PKK20_07580"
     CDS             complement(1428..2117)
                     /locus_tag="PKK20_07580"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNU99786.1"
                     /translation="MPTWKRVLDVFCITAALPLIAPVMGLVALLIKCVSKGPVLFRQE
                     RVGYRGRRFTCLKFRTMIVGANTEVHEAHCRQLIATGAPMTKLDDEEDDRIIPFGQVL
                     RSTGLDELPQLLNVFQGDMSLVGPRPCLPAEYASYLPEQRRRFDAVPGLTGLWQVSGK
                     NSTTFARMIELDLDYARNRRLFLDLEILVRTLPVVVAAGAKAVRRRISRSRLFRNLIH
                     SPLQGNRSYAG"
     gene            complement(2867..3694)
                     /locus_tag="PKK20_07585"
     CDS             complement(2867..3694)
                     /locus_tag="PKK20_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNU99787.1"
                     /translation="MDDLDSLFNVRGQVAVITGGAGVLCSAMAKGLAAAGAKIAVLDL
                     AREAACKLADEIKAAGRTAVGLPCNVLDRPSVEAAAEAVLQAFGRVDVLVNGAGGNKP
                     QATTSPDKRFFDLPADAVQWVFNLNFVGTLLPSQVFGRLMARQQSGVIINVSSMNAFR
                     PLTRIPAYSAAKAAVSNFTQWLAVHMAQEYSPAIRVNAIAPGFFLTEQNRFLLTDADT
                     GALTSRGQQIIGHTPQGRFGTPDDLVGTLLWLVSPGAGFVTGIVVPVDGGFSAYSGV"
     gene            3798..4922
                     /locus_tag="PKK20_07590"
     CDS             3798..4922
                     /locus_tag="PKK20_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU99788.1"
                     /translation="MHLGSSYKARHNIMHASSADQPASSQSIFASVTFYHWMVVIIAS
                     CGWLFDCMDQRLFTMARESTLKELLGNNPAGLAKLESYLGLATAAMMVGWATGGIVFG
                     MLSDKWGRVKTMVVTLMVYSGFTGLFGLAQSWVDFTLYRFLVGPGVGGMFGAATTLVA
                     ESVPGRFRAVALGSLQALSATGNLLGAYISLRIPPGTEHFFGSFSGWRVLFFVGIVPA
                     LLVIPIITILKEPEAWKRAKAAAATGDASKNIGSPRDLLRHPRWRRNTLVGLGLGLAG
                     MVGLWGIGFFSPELITTALKGRAVRAEDIRDGAALCKALRFETNAAVAYLSKQLSAET
                     RGLLAQVESGAAASPELMETMRADLNRIIEGGSLYDSGAF"
     gene            4932..5525
                     /locus_tag="PKK20_07595"
     CDS             4932..5525
                     /locus_tag="PKK20_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99789.1"
                     /translation="MDKRTQGLLKKVQATAAPADVRFLNRQLVEQAIPGGLLSLQKSI
                     DQVRSKAMMLFDVGSFAGMIAFTIFAAWLSRRGAFFWAFLACLAITGYVFYALKTETD
                     AYWMLPLMGFAQLAVFAGYSIYFPELYPTRLRGTGVGFCYNTVRYVAAPFPYLLGYMS
                     TLLSFRTAAIVMSGIYLFGIVALVWAPETKGQPLPED"
     gene            complement(5560..6624)
                     /locus_tag="PKK20_07600"
     CDS             complement(5560..6624)
                     /locus_tag="PKK20_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNU99790.1"
                     /translation="MSELRDRWHGRGAASNPPNRFRRLVLEPDADWNPAQDSAPATQF
                     LLDRSQSIISCNDSPDLGFSASLNPYRGCEHGCAYCFARPTHEHMGFSAGLDFETRIV
                     VKDKAPELLRREMASARWQPQVLALSGVTDPYQPVERRLEITRRVLRILAEFRNPVCL
                     VTKNALVARDRDVLADLAQHRAAAVNLSITTLDSDLARKLEPRATLPVHRLAAIRQLA
                     QAGVPVGVLVAPVIPGLTDHEIPSIIRAAAEAGAGWACTLMVRLPLAVGPLFTAWLEH
                     HAPGLKDKVLGRIRAMRGGELNDARFRKRMRGEGVLAEQIAQLGRVARRRAGLARESP
                     ELSIAAFRRPGGKQMEMNLG"
     gene            complement(6758..>6886)
                     /locus_tag="PKK20_07605"
     CDS             complement(6758..>6886)
                     /locus_tag="PKK20_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017216320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNU99791.1"
                     /translation="HGADLRVIQELLGHANISTTEVYTHVAGERLRTIHQKYHPRA"
BASE COUNT         1262 a         2131 c         2162 g         1331 t
ORIGIN      
        1 cctcgatgat cacgccttcg cagatgaagg tgtgtctcga gaccttgcag cgccgaccga
       61 tcttggcacc tttctgcacc tcgacgaatg tgccgatctt cgagtcgtcg ccgatctcgc
      121 agccgtagag gttggtgaat ccgtagagac gcaccccatt gcccagaatc acatcaggcg
      181 caatcagccg gtaactggaa tcgaatgtgc tcatagactg tcgtccgttc gaagtcatgc
      241 cgcggccagg gaaaccgcta cctcttttcg aggcgggcgc ccgttgcgtt tcgatgcggc
      301 atgggcgcgg ggggcagtgc tcttcaccat gctcgtggcg ggagcgtggt cggcttcctt
      361 gtcgaagtgc accggcccgc cctcctgctt gagcgaggcg gacgcggctt cgaggatgcg
      421 gaccatggcg agcgcctgtc ggccgtcggt gagggggcgt gtcccgttgc ggatgcaatc
      481 gaggaaatgc tggcactcga ccttcagggg ctcctcctgc ttgatgtagg gcgcgtacac
      541 gtcgccgtag tgatacgcat aatggaactc ggcgaaggtg tcgtagtggg gcgggcgctc
      601 gactcgcgcg tcgaatatgc gaatcttctc caactgagcg acatcgtcgt acactatcat
      661 ccgccggctc ccgacaatcg tcatctcgcg aaccctgcgc ggatcgagcc aactgctgtg
      721 gataatggcc gaccgctcct ttcggaatgt gagaaacagg ctggtcacgt cctcgatgcc
      781 cggggtaacg tgggcgctgc cgcgacaatt cacgctcaga ggcgactcct ccatgatatg
      841 gaggatgatc gagatgtcgt gcggcgcaag gtcccacgtc acgttgatgt ccctctgata
      901 caaaccgaga ttcaaccggc gagcggagat gtaacgaatg tcgccaatgt cgccggcatc
      961 gacgatttcc ttgatcttcc ggaccgcggg cgaatagaga aacgtgtgcc cggccatcag
     1021 caccaaaccc ttgccgtccg ccagatggat caggtcctcg cattcggccg atgaggcagc
     1081 catcggcttt tcgataaagg tgtgcttgcc ggccgacagg ctcgctttcg ccatgcgatg
     1141 atgaaactcg accggggtgg cgatcacaat cgcatcgagg tcctttcggg tcaccatcgc
     1201 attatagtcg gtcatggtgc gcgcctcggg atagaggctg cccaggtgtt tcagacgctc
     1261 ttgcttgacg tcacaaatca cctcgagcct gcagttggac aaggaccgca gatttcgcat
     1321 gagattcggt ccccagtaac cgcatccaac aactcccact ctgattggcg taggcatgat
     1381 gttctcctcc ctgtttattg cattgtgtgc ttttgcttcg gacgtcctca gccggcatac
     1441 gaccgattcc cctgcagagg gctatgaatt aggtttctga acagcctcga acggcttatc
     1501 cgtcggcgaa ccgccttagc ccctgccgca accacaaccg gcaaggtcct cacaagaatc
     1561 tccagatcca gaaagagccg ccggtttcga gcgtaatcga ggtccaactc gatcatccgg
     1621 gcgaacgtgg tgctattctt gccgctgacc tgccagaggc ccgtcagacc cggcaccgca
     1681 tcgaagcgcc gcctctgttc gggcaaatag gacgcgtact cggcgggcag gcagggtcgg
     1741 ggccccacga ggctcatgtc cccctggaac acgttcagaa gctgaggcaa ctcgtccagt
     1801 ccggtcgaac ggagcacctg tccaaaggga ataatccggt cgtcttcctc gtcgtcgagc
     1861 ttcgtcatgg gcgcgccggt ggcgatcagt tggcggcagt gcgcctcatg cacttcggtg
     1921 tttgccccga cgatcatggt ccggaacttg aggcacgtga accgcctgcc gcggtacccg
     1981 acgcgttcct gtcggaacag gaccggccct ttggacacgc acttgatcaa cagagcgacc
     2041 aagcccatga caggggcaat caggggcagg gcggcggtga tacagaagac atccaggaca
     2101 cgcttccacg tcggcacctc ctgttcacgc cgcctgcgga tcgaggccgg cgcaagagcc
     2161 ggtggcccgg ccgtgtcatc catgcaaggc cggccgagag atagggtggc cttacagtac
     2221 acacccggat taatgctcgc atttacgcct aaaatcataa aaaccgagcg cacttttaca
     2281 aactgtcgat aaacaataca atcggaatga ataagtagtt aatactgagt agtgatactt
     2341 aattaactcc tttatgcgac cctcgcgtca gatgaaccgg cttctttcaa gctttgcccg
     2401 ccctccgcag ccgtccccgc ggctgagatg cggaccttgc atcgaagctg cccgatcggg
     2461 aacgtgtgcg gtagcgcgaa gtcccgcggc tgcggggctt cgcgacctcg acgccgacca
     2521 gagcatgggc gagtgtcaca acaaactccg gccaagtact tagacgacag cgatggcgct
     2581 gctctcagga acagcgcggg gagatcgcgt aagcagagac cggagtcgga cggtcagacc
     2641 cgcctctggc ggctggccaa gcgccggatt cggtgagcct tgccgcaggc aggtgggtcg
     2701 actgaggacc gatgtcgcaa agcggagtgc gcccgtcctc gggcgcagcg gtgtccgagc
     2761 aaccgcgcgc gtcggtctgg tccacatcat ggcgcattcg gtcattgctg tggccgagga
     2821 cggccaccct ccagtcaccc cgacgacgca gtgctctccg gaaaacctaa actccgctat
     2881 atgcggagaa gccgccgtcg acgggcacga caatgccagt gacaaagcca gcacctggcg
     2941 agacgagcca gaggagcgtg ccgaccaggt catcgggcgt cccgaaccgc ccctggggcg
     3001 tatggccgat gatctgctgg ccgcgggaag tcagtgcacc ggtgtcggcg tcggtcagca
     3061 agaagcggtt ctgttcggtc aggaagaagc cgggggcaat ggcgttgacc cgtatggcgg
     3121 gcgagtattc ctgggccatg tggacggcca gccattgggt gaagttgctg acggcggctt
     3181 tggcggctga ataggccggg atgcgcgtga gaggtcggaa ggcattcatc gaggacacat
     3241 tgatgatcac cccgctctgc tggcgcgcca tgaggcgtcc gaacacctgg ctggggagca
     3301 acgtcccgac gaagttcaga ttgaagaccc attgaacggc gtccgcgggc agatcgaaga
     3361 agcgtttgtc cgggctggtg gttgcctggg gtttgttgcc accggcgccg ttgacgagca
     3421 cgtccacgcg cccgaaggcc tgcaggacgg cctccgcggc ggcctcgacg cttgggcgat
     3481 cgagcacgtt gcatggcaga ccgacggctg ttcttccggc ggccttgatc tcgtcagcca
     3541 gcttgcaggc cgcttcccgc gcaagatcga gcacggcgat ctttgcgccg gcggcggcca
     3601 ggcctttcgc catggccgag caaaggacac cggccccgcc ggtgatcaca gcgacttgtc
     3661 cacgcacgtt gaagagggaa tcgaggtcgt ccatatcgag ttcaggttag gggctccgcc
     3721 atcagtcttg gccgggtccc gccaccggcg gcactatacg attgtccagg ctgacgctga
     3781 agcagtaaat gcttcccatg catctaggga gcagctacaa ggcccggcat aacatcatgc
     3841 atgcctcttc cgccgaccag cccgcctcga gtcagagcat tttcgccagc gtgacgttct
     3901 atcactggat ggtggtgatc atcgcttcct gcggttggct cttcgactgc atggaccaac
     3961 ggctcttcac catggcccgc gagtcgaccc tgaaggagtt gctcggcaac aatccggcgg
     4021 gcctggccaa gctcgaaagc taccttggct tggccaccgc ggcgatgatg gtcgggtggg
     4081 ccacaggcgg catcgtcttc ggcatgctca gcgacaaatg ggggcgggtg aagacgatgg
     4141 ttgtgacgct gatggtctat tccggcttta cggggttgtt cggcttggcg cagagctggg
     4201 ttgactttac attgtatcgc tttcttgtcg gtccgggtgt cgggggaatg ttcggcgcgg
     4261 caacgacgct cgtggccgaa agtgttcccg gacgattccg cgcggtggct ctgggctcgc
     4321 tgcaagccct ctcggccacc ggcaacctcc tgggcgctta catcagcctt agaatcccgc
     4381 cgggcaccga acattttttc ggcagcttca gcggatggcg cgttctgttc ttcgtcggca
     4441 tcgtcccggc cctgctggtg atcccgatca ttacgatcct gaaagagccg gaggcgtgga
     4501 agcgcgccaa ggccgcggcg gccacgggtg atgcatcgaa gaacatcgga tccccccgcg
     4561 atctgctcag acacccgcgg tggcgacgca atacactcgt cgggctcggc cttggcctgg
     4621 cggggatggt cgggctttgg gggatcgggt tcttctcgcc cgagttgatt acgacggcgc
     4681 tcaaggggcg ggctgtgcgg gccgaggaca ttcgggacgg ggccgcgtta tgcaaggcgt
     4741 tgcgcttcga gacgaatgcg gctgtggcgt acctgagcaa gcaactctcg gcggagacca
     4801 gaggcctctt ggcgcaagtc gaatccggcg cggcagcctc acccgaactg atggaaacga
     4861 tgcgggcgga tctgaatcgg atcatcgagg gaggcagtct ctacgattcg ggcgcattct
     4921 agaacgtgac gttggacaag cggacgcaag gtctcctgaa gaaagtgcag gcgacagcgg
     4981 ccccggcgga cgtgcggttt ctgaaccggc agttggtcga acaagcgatt cccggcggcc
     5041 tcctctcgct ccagaagtcg atcgatcagg tccgcagcaa ggcgatgatg ctgttcgatg
     5101 tcggctcatt tgcgggcatg attgcattta ccattttcgc cgcctggctg agccggaggg
     5161 gggcgttttt ctgggcgttt ctggcctgcc tggccatcac ggggtacgtc ttttacgcgc
     5221 tgaagaccga gaccgacgcc tactggatgc tgccgctgat ggggtttgcg cagcttgccg
     5281 tgttcgccgg ttactcgatc tatttccccg agttgtatcc gacccgcctg cggggcacgg
     5341 gtgtcggttt ctgctacaat accgtccgct acgttgccgc gccgttcccc tacctgctgg
     5401 gctacatgag cactctgctc tccttccgca cggcggccat cgtcatgtcc ggcatttatc
     5461 tgttcggcat tgtcgcactg gtgtgggcgc ctgaaaccaa agggcagcct ctgccggagg
     5521 attgaccgga agccgctccg ggtgccgatc gagtgcgcct catcccaggt tcatttccat
     5581 ctgtttgccg ccgggccggc ggaatgccgc gatcgacagt tccggactct cgcgcgccag
     5641 cccggcccgc cgacgggcca cccgcccaag ttgagcaatc tgttccgcaa gcacgccctc
     5701 tcctcgcatc cgtttgcgga aacgcgcgtc gttcagttcg ccgccccgca tggcccggat
     5761 gcgtccaagc actttgtcct tcagccctgg cgcgtggtgt tcgagccacg cggtgaacaa
     5821 cgggccgacg gccaggggca accgcaccat cagcgtgcag gcccagcccg cgccagcctc
     5881 ggccgcagcc cggatgatcg acgggatttc gtggtcggtg aggcccggga tcaccggagc
     5941 gacgagcaca cccaccggaa cgccggcctg ggcgagttgt cggatcgccg ccaaccgatg
     6001 gacaggcagc gtcgcccgcg gctcgagttt ccgcgccaaa tcgctgtcaa gcgttgtgat
     6061 cgacagattg acagcggcgg cccgatgctg cgccagatca gccagcacat cccgatcacg
     6121 cgcgaccagt gcgttcttgg tgaccaggca gaccgggttc ctgaactcgg ccaaaatccg
     6181 cagcacacgt cgcgtgatct cgagtcggcg ctcgactggc tggtagggat cggtcacgcc
     6241 gctcaaggcc agcacctgcg gctgccagcg ggccgacgcc atctcgcggc gcagaagctc
     6301 cggggcttta tccttcacca caatccgcgt ctcgaaatcc agccccgccg aaaaacccat
     6361 gtgctcgtgc gtcgggcgcg caaaacagta ggcgcagccg tgctcgcagc cccgatacgg
     6421 attcaggctc gccgagaaac ccaggtcagg actgtcgttg caagagatga tcgactgcga
     6481 acggtccagc aagaattggg tcgcaggagc cgaatcctgg gctggattcc agtccgcgtc
     6541 cggttcaagc accaggcgcc tgaatcggtt cggcgggttg ctcgccgccc cgcgtccgtg
     6601 ccacctgtca cgcagttctg acacgctccc aagttgggcg gagccattgt cggacgcaac
     6661 tgcgttctgc gcactgcatt ctggcactgc aatcgctcag attgctccca ggccgagaca
     6721 cgaagagaaa ggaaccgctt ggcagggcga tgcgtgctca cgctcgcgga tgatacttct
     6781 ggtgtattgt ccgcaaccgt tcgccggcca cgtgcgtgta gacttccgtg gtgctgatgt
     6841 tcgcgtgccc cagcagttcc tggatcaccc gcagatcggc tccgtg
//
LOCUS       DAOQRO010000170         6795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11063_length_6795_cov_4.282196, whole genome shotgun sequence.
ACCESSION   DAOQRO010000170 DAOQRO010000000
VERSION     DAOQRO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11063_length_6795_cov_4.282196"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PKK20_07610"
     CDS             complement(<1..1134)
                     /locus_tag="PKK20_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNU99792.1"
                     /translation="MKLVKIAAAVLNQVPLDWRHNLANVRAVLEDARAAGVSVVCLPE
                     LCLTGYGCEDAFHCADTHRRAWRALAALVPEIGGLVVSFGLPVLHQNAVFNVAALVVD
                     GRLAGLVAKRFLAGDGIHYEPRWFKPWPAEVRDTFVAPEPCGGERQFPIGDLLFDVGG
                     VRMGFEICEDAWVANRPGAALARRGVDLILNPSASHFAFGKLEARRRFVLEGSRAFGA
                     SYIYANLVGNEAGRAIFDGGALIASGGRLLAAGPRLGFLDRDLTLGVVDIEATRMNQA
                     RTGSYQPELPGAHALEVRTDFDWPELTPDQARIRNVPGSVQSRSAGPPASAAPVSPDS
                     GDVSWESSATLKEEEFTRAVALALFDYLRKSRSQGFVVSLSGGA"
     gene            complement(1136..2581)
                     /locus_tag="PKK20_07615"
     CDS             complement(1136..2581)
                     /locus_tag="PKK20_07615"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNU99793.1"
                     /translation="MPATALLTDLYQLTMAYGYWKTGRDQREAVFHLFYRNNPFRSGF
                     AIAAGLSDVVDYLRNLRFAPDDLAYLGSLTGQDGRGLFEAGFLDHLAGLRLTCDIDAV
                     PEGTVVFPHEPLVRVRGPILEGQILETALLNQINFQTLIATKAARVCLAARGEPVLEF
                     GLRRAQGVDGALTASRAAYIGGCSATSNVLAGKTYGIPVRGTHAHSWVMSFDTEREAF
                     QAYAEAMPHNCVFLVDTYDTLQGVREAIGVGGWLRERGHALAGIRLDSGDLAWLSIEA
                     RKLLDEAGFSKTAIVASNDLDEHLIESLKGQGAAIGIWGVGTKLVTAYDHPALGGVYK
                     LGGIRRPDGTWEPKMKLSEQAVKISTPGILQVCRFRSDTECVADGIYDEERGAPPEFT
                     IVDPVDPTRRNRFAPATARETLLVPVFRRGECVYSSPALPTIRERVRSQLSLFPPGIK
                     RLANPHRYPVGLELGLHERKTALIMQARGLS"
     gene            complement(2963..3964)
                     /locus_tag="PKK20_07620"
     CDS             complement(2963..3964)
                     /locus_tag="PKK20_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNU99794.1"
                     /translation="MQPRTETKIVLVIRPSRLAELVGRFNTVAQARFYVEHLGADFTD
                     YEREDARYGEALRQAKQRIAEWGRIQVLERRFLPNFIFGPQDVIVALGQDGLVANTLK
                     YLDGQPLIGVNPDPQRWDGQLLPFRVEDLTALMPEVLAGRRSTRAVTLAQARLNNGQS
                     LCAVNDLFVGLRTHGSARYRIRIGDAAEDHSSSGVIVSTGLGSTGWLQSLLGGATAIS
                     RSAQAAFYGLSMSAGAGTTAPEPAPLAQAEARARWQFPWDADYLYFTVREPFPSRRTG
                     CALVFGRITADTSFSLESQMPENGVIFSDGIEHDFLEFNSGARAAIGLASRKGLLVV"
     gene            complement(4177..5199)
                     /locus_tag="PKK20_07625"
     CDS             complement(4177..5199)
                     /locus_tag="PKK20_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU99795.1"
                     /translation="MFGIRYIKVPPTTYLLQYRRGQLVREGPGLAFFYYAPVTSLVAV
                     PSGSADVPFMFEERTADFQTVTVQGHITYRVADPKRLAGLLNFTLAGDGSTYTSEDPG
                     MLPQRVLHVANELVRAQLQTLPLCEAINASARLVVAVQAGLAGSPALTTLGLEFLGLS
                     ILAIRPSPETARALEAEARERLLRAADEAIYARRNAAVEQERAIKESELNTEIAVENK
                     KRQIRETQMDAERAVLEKQHLLQQSDMAAQIQLESDRRNLVSLATANARTEADARAYG
                     IAATMQALAAADPRVIQALASTGMKPEQLIAAAFQDLAGKAERIGQLNVSPDLLRELM
                     DREGGR"
     gene            5440..5619
                     /locus_tag="PKK20_07630"
     CDS             5440..5619
                     /locus_tag="PKK20_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99796.1"
                     /translation="MDLPLTPAGHAARPGIPQAALTTNRWLHVAATYSGNAGLAGGQT
                     VTYLDGQAADIHVGH"
     gene            complement(5662..>6795)
                     /locus_tag="PKK20_07635"
     CDS             complement(5662..>6795)
                     /locus_tag="PKK20_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNU99797.1"
                     /translation="LVQTPGKIVGEKSDRLLASYAACHLERMGDFQAGFVTPLLGSGA
                     ASLLIFVEQDWGTALLLIGASVIVLTVAGTRPVYLGGAGLGGLGLLTTLARHDPHRWA
                     RLICFLDPERYQDGIGAQQWHGLLSLGTGGLFGGFFGSGRHKFGFVPEQHTDFIFSLI
                     GEELGLSGTLLVLGLFFLVFKSGLWIAWGAGDPFGRLLAAGLTSLITCQALVNMGVVT
                     CLLPNKGIPLPFISFGTSDLLCMCVATGLLVSIARRSPGLPAGQAPPLSPEDDAARER
                     RAALHALPLGDEKRPWWKRISLRLGRVGARNAFRAARSYQRPPRAIAVRPTEGTRHCE
                     RVGSWKTLKVNDLMRIGPVKKETFNAQLPTLDGQRSGSLKAGR"
BASE COUNT         1271 a         2248 c         2098 g         1178 t
ORIGIN      
        1 cgccccgccg ctgagcgaca cgacaaatcc ttgggaccgg ctcttgcgca ggtaatcgaa
       61 cagggccagg gccacggccc gagtgaactc ctcttccttg agcgtcgcgc tcgattccca
      121 ggacacatcg ccggaatccg gagaaacggg cgctgccgac gcaggcggac ccgccgatcg
      181 actctggaca gacccaggca cattgcgaat ccgggcctgg tccggggtca gttcgggcca
      241 gtcaaaatcg gttcggacct cgagggcatg cgcccccggc aattcgggtt ggtaactgcc
      301 ggtgcgggcc tggttcatcc ttgtggcctc gatgtccaca accccaagtg tcagatcccg
      361 atcgagaaaa ccgagcctcg gccccgcggc aaggaggcgt ccgcccgacg cgatcagcgc
      421 accgccgtcg aaaatcgcgc ggccagcctc attgcccacc aggttggcgt agatgtaact
      481 tgcgccaaag gcccgtgagc cttcgaggac gaagcggcgc cgggcttcga gcttgccgaa
      541 tgcgaaatga ctggcgctcg ggttgaggat caggtcgacc ccgcggcgcg cgagggccgc
      601 gcccggccgg ttcgccaccc acgcgtcttc gcagatctcg aatcccatcc gcactccgcc
      661 cacgtcgaaa agcaggtcgc cgatggggaa ctgccgctcg ccgccgcaag gctccggggc
      721 gacaaaggtg tcgcggacct cggcaggcca cggcttgaac caccgcggct cgtagtggat
      781 gccgtcaccg gccagaaacc gcttcgcgac caatccggcc aggcgcccgt ccacaaccag
      841 ggccgccacg ttgaacacgg cgttctggtg caagaccggc aatccgaacg agaccaccag
      901 accgccgatc tcggggacaa gcgccgccag cgcgcgccag gcgcgccggt gggtatccgc
      961 gcagtgaaag gcgtcctcgc atccgtaccc ggtcaggcac agctcgggca ggcagaccac
     1021 gctgacaccc gcggcgcggg cgtcttcgag aacggcgcgc acattggcca ggttgtggcg
     1081 ccaatcgagc ggcacctgat tgaggacggc agcggcaatc ttcaccagtt tcatgtcatg
     1141 acaaacctcg ggcctgcatg atgagcgcgg tctttcgctc gtgcaggccc agttccagcc
     1201 cgacggggta ccgatgcgga ttggccaggc gtttgatgcc cggtgggaag agagagagct
     1261 gactgcggac acgctcacgg atcgtcggca aagccggcga tgaatacaca cactccccgc
     1321 ggcgaaacac cggcaccagc agggtctcgc gtgccgttgc cggggcaaac cgattgcggc
     1381 gcgtcggatc caccggatcc acgatggtga actccggcgg cgcgcctcgt tcctcgtcgt
     1441 aaatgccgtc cgccacacac tccgtgtcgg accggaagcg gcagacttgg agaatgcccg
     1501 gcgtggaaat cttcaccgcc tgctcggaca gtttcatctt gggttcccat gtgccgtccg
     1561 ggcggcggat gccgccgagt ttgtagactc cgcccaaagc ggggtggtca taggccgtca
     1621 cgagcttggt gcccacgccc caaatgccga tggcggcccc ctgtcctttc aggctctcga
     1681 tcaaatgctc gtccagatcg ttgctggcga cgatggcggt tttcgagaac ccggcttcat
     1741 cgagcagttt gcgggcttcg atgctcaacc aggccaaatc tcctgaatcg agccgaatcc
     1801 ccgccagcgc gtggccgcgc tcgcgcagcc acccgcccac accaatcgcc tcacgcacgc
     1861 cctgcagcgt gtcgtaggtg tcgaccagaa aaacgcaatt gtgcggcatg gcctcggcat
     1921 aggcctggaa tgcctcgcgc tcggtgtcga agctcatcac ccacgaatgc gcatgggtgc
     1981 cgcggacggg aatgccgtag gtcttaccgg ccagcacatt cgatgtggcg ctgcaaccgc
     2041 cgatgtaggc ggcccggctg gccgtcagcg cgccatccac cccctgcgcc cgccgcagtc
     2101 caaactcgag caccggctcg cctcgcgccg ccaggcagac ccgcgccgcc ttggtggcga
     2161 tcagtgtctg gaagttgatc tgattgagaa gcgcggtctc gagtatctgc ccctcgagaa
     2221 tcgggccgcg cacgcgcaca aggggctcgt gcgggaacac aacagtcccc tccggcaccg
     2281 cgtcgatgtc gcatgtcagc cgcagcccgg ccagatgatc cagaaaaccg gcctcgaaca
     2341 acccccgacc atcctgcccc gtcaacgacc caagatacgc caggtcgtcc ggagcgaacc
     2401 tcaggttgcg caggtagtcc accacgtcgg ataaccccgc cgcgatcgca aaaccgctcc
     2461 gaaacggatt gttccggtag aacaaatgaa acacggcctc gcgctggtcc cgcccggtct
     2521 tccagtaacc gtaggccatg gtgagctggt acaagtcggt cagaagagcg gtggcaggca
     2581 taatgggtgg cgacgaggat tccagacagc cctcggtccg ctcctgcgtc tcatgcatct
     2641 tgccagccac aacccggacg caggctggcg ccagagagtg gccccgcgat tagcggcgca
     2701 gggagaggtg gcaatggagc gcatcctggt gatttccgtc tattgatggg tcggaggtta
     2761 gacgcttgtt gagccatttg gcaattcgga aagagaacgt tgaaagctca actcagccgc
     2821 cgcctatgtt tgcgatcctg gctgtgcgcg gctttgatgc ccaccaacga gattcagcct
     2881 ctccaaggcg attccagata ttcgatttgt cggtgccgcc tttgatctcg atggccacaa
     2941 tgctcttcgc tccccctcgt gtttacacaa caagcagacc ctttcggctc gccagcccga
     3001 tggccgcgcg cgcgccggaa ttgaattcga ggaagtcatg ctcgatgcca tcgctgaaga
     3061 tgacgccgtt ctctggcatc tgggactcca agctgaatga cgtgtcggcg gtgatccttc
     3121 cgaaaaccag ggcgcacccg gtgcgccggc tggggaacgg ctcgcgcacg gtgaaataca
     3181 agtagtcggc atcccaggga aattgccaac gggcgcgtgc ctcagcctgc gcgagggggg
     3241 ccggctcggg ggcagttgtt ccagcccccg cggacatgct caatccgtag aaggccgcct
     3301 gggcgctgcg gctgatcgct gtggcgccgc ccaacaggct ttgcagccag ccggtcgagc
     3361 ccaagccggt cgagacgatg acaccactcg aggaatgatc ctcggcggca tcgccaatcc
     3421 ggatgcggta gcgagccgaa ccatgggtgc ggagcccgac gaagaggtcg ttgacggcac
     3481 acaggctctg gccgttgttg agtctcgcct gggccagcgt gacggcgcgc gtcgagcggc
     3541 gcccggccag gacctccggc atgagcgcgg tcagatcctc gacgcggaac gggagcaact
     3601 gtccgtccca gcgctggggg tccgggttga cgccgatcag gggttgcccg tcgaggtact
     3661 tgagtgtgtt ggccaccagg ccgtcctggc cgagggcgac gatcacatcc tgcggaccga
     3721 aaatgaaatt gggcaggaag cggcgctcga gcacctggat tcggccccat tcggcgatgc
     3781 gctgcttggc ctggcgcagc gcctccccgt accttgcgtc ctcccgttcg taatcggtaa
     3841 agtcggcgcc cagatgctcg acgtagaatc gcgcctgggc aaccgtgttg aatcgcccca
     3901 ccagctccgc gaggcgactg ggccgaatca ccaggacaat cttggtttct gtgcgtggtt
     3961 gcatatggcg ccaacacctg actcatttgc cacagatttc atttggagaa tcactctaac
     4021 gaggcgcacc tcagaccaaa tccaatcctt ctttccatga ggagtccccg tgtttcctcg
     4081 ccctctctcc cacgcctcgt tccttagaat ccttgacccc ttcaacgcag cacagaagct
     4141 tgacttgtct gcaacgagtt tcaccggtca aggattctag cgaccgcctt ctctgtccat
     4201 gagttcgcgc aagaggtccg gcgagacgtt gagttggccg atgcgttcgg ctttgccggc
     4261 cagatcctgg aacgcggctg caatcagttg ctcgggcttc atcccggtgc tggccagggc
     4321 ctggatcacc cgcggatcgg ccgcggccag cgcctgcatg gtggccgcga tcccgtaggc
     4381 gcgcgcgtca gcctcagtcc gtgcattcgc agtggccagg ctgacgagat tccgccgatc
     4441 cgactcgagt tggatctgtg cggccatgtc cgattgctgc aagaggtgct gcttctcgag
     4501 tactgcgcgt tcggcatcca tctgggtctc gcggatctgg cgtttcttgt tctcgaccgc
     4561 gatctcggtg ttcaactcgc tttccttgat ggcccgctcc tgctcgacgg cggcgttgcg
     4621 gcgcgcatag atcgcctcgt cggccgcgcg caacaagcgc tcgcgcgcct ccgcttccag
     4681 ggctcgcgcc gtctccggcg acggccggat ggcgagaatc gacaagccga ggaattcgag
     4741 cccgagggtc gtcagggcgg gcgagcccgc cagcccggcc tggacagcca cgaccagcct
     4801 ggccgaggcg ttgatggctt cacacagcgg cagagtctgg agttgggcgc gcaccaactc
     4861 attcgcgacg tggagcacgc gctgcggcaa catgccgggg tcttccgagg tgtaggtcga
     4921 tccgtcgccg gccagcgtga agttcagcag ccccgcaaga cgcttagggt cggccacacg
     4981 atacgtgatg tgtccctgga ctgtgacggt ctggaaatcg gccgtgcgct cctcgaacat
     5041 aaacggcaca tcggcgctgc ccgacggcac ggcgaccagc gacgtgaccg gggcgtaata
     5101 gaaaaaggcg agaccgggcc cctcgcgcac gagttggccc cggcgatact gaagcaggta
     5161 ggtggtgggt gggactttga tatagcggat gccaaacata acaactcctt ggttcgtgtt
     5221 gctctatttt ttggacgcgc cctcctcggc gaacccgact atagatacgc gaggtgatcc
     5281 gggatgtcaa atcaggaccg acagaatgct ccccgttcca tttctggtcg cggttttgtc
     5341 agagcccgga tgtcagactc cgcgaaactt tgtgatacat ccaggctagc cagcgtgccc
     5401 ccacgcggcc tgcaacatcg gtgtctgtct tcgcgccgca tggatctgcc tttgactccc
     5461 gcggggcatg ccgcccggcc gggcattccc caggccgctc tgacgacaaa ccgctggctc
     5521 catgtggcgg ccacatacag cggcaacgcc gggctggccg gcgggcagac cgtcacctac
     5581 ctcgatggcc aggccgccga catccacgtc ggccattgat gatcaataga gggtccaact
     5641 cgaacgtcca acgcgcaacg ttcaacgtcc agctttcaac gaaccgcttc gttgaccgtc
     5701 gagcgttgga agttgggcgt tgaacgtttc ctttttcacg ggtccaatgc gcattaagtc
     5761 gttgactttc aatgttttcc atgagccgac acgctcgcag tgccgagtgc cctctgttgg
     5821 tcgcacggca atcgcgcgcg gcgggcgttg atagctgcgc gccgcgcgga aggcgtttcg
     5881 cgcgccgacc ctgccaagcc gcaggctgat ccgcttccac cagggacgct tctcgtcgcc
     5941 cagcggcagc gcatggaggg cggcccgccg ttcgcgcgcg gcatcgtctt caggagagag
     6001 tggcggcgcc tggccggcgg gcagccctgg cgagcgcctg gcaatcgaga cgaggagccc
     6061 cgtagccacg cacatgcaga gcaggtctga cgtgccaaag ctgataaacg ggagggggat
     6121 tcccttgttg ggcaacaggc acgtgaccac gcccatgttc accagggcct ggcacgtaat
     6181 caggctcgtc aagcccgctg ccagcaaacg gccaaaagga tcaccggctc cccaggcaat
     6241 ccacaacccc gacttgaaca ccaaaaagaa caaaccgagg accaacagcg tccctgaaag
     6301 ccccaattcc tcgccaatta acgaaaagat gaaatccgtg tgttgctcag ggacgaaccc
     6361 gaacttgtgt cgaccgctgc cgaagaagcc gccgaagagc ccgccggtcc ccaatgacag
     6421 caacccatgc cattgctggg cgccgatgcc gtcctggtag cgttcggggt ccagaaagca
     6481 gatcaggcgc gcccagcgat gcggatcgtg ccgcgccagg gtcgtcagaa gtccaagccc
     6541 tcccaacccg gccccgccca ggtagacggg gcgcgtcccg gccaccgtga gcactatcac
     6601 gctcgcgcca atcagcagca gcgctgttcc ccaatcctgc tcgacaaaga tcagaaggct
     6661 tgcggcgccg cttcccaaca acggcgtgac gaatccggcc tgaaaatcgc ccatccgctc
     6721 caggtggcac gcggcgtagc tggctagcag cctgtcggac ttttccccaa caattttgcc
     6781 cggcgtttga acaag
//
LOCUS       DAOQRO010000171         6769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11125_length_6769_cov_5.301609, whole genome shotgun sequence.
ACCESSION   DAOQRO010000171 DAOQRO010000000
VERSION     DAOQRO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11125_length_6769_cov_5.301609"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1083)
                     /locus_tag="PKK20_07640"
     CDS             complement(124..1083)
                     /locus_tag="PKK20_07640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNU99798.1"
                     /translation="MLAKRGRIGDCFSKDERIVVFPGDCLELLRTCPDETFRLIVTSP
                     PYNIGKEYEKKLELDSYLSQQAEVIRECVRCLSPRGSICWQVGNYVDGGAIIPLDTVL
                     YPIFRELGLSMRNRIIWHFEHGLHCSRRFSGRYETISWFTKSDDYVFNLDPVRVPQKY
                     PGKKYFKGPKAGQFSCNPLGKNPGDLWVIPNVKSNHVEKTEHPCQFPVELVERLVLSL
                     AEEDDWVLDPFLGTGTTIVAAVRHGRRAAGAETVPKYVSLARERIEGELAGTLRTRPM
                     HKPVYDPVEAGNSLTAAPWTAEGDTAQMVLLEKKRSYGRSGTP"
     gene            complement(1375..2418)
                     /locus_tag="PKK20_07645"
     CDS             complement(1375..2418)
                     /locus_tag="PKK20_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNU99799.1"
                     /translation="MKDHLQPWSVNADDFPESGYASDQLEFLLNYAILAPSSHNTQPW
                     LFRINAMDVELFADRRRALRVIDPCDRELTISCGAALYNLRIAAEYFGRKYDVSLFPN
                     PNLPDLLAQLRLGLSGETSSDDVLVFHAITQRRTNRQAFRPDPVPTELLAELESLTQA
                     EGAWLSAYAVEEVRNAVADLVAQADRMQWADKAFREELARWVRTKAESHRDGLPVHDL
                     GIKDWMSFAGPALIRTFDRGGGQAARDRELATHSPVLAVLWTPEDNAPTWLNTGQALQ
                     SLLLHARSENLWASFLNQPIEIPELRAQLASMLGLAGYPQILLRLGYGEETPPSPRRA
                     VRELLVMHKTAHH"
     gene            complement(2540..5158)
                     /gene="clpB"
                     /locus_tag="PKK20_07650"
     CDS             complement(2540..5158)
                     /gene="clpB"
                     /locus_tag="PKK20_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNU99800.1"
                     /translation="MDLNKFTEKSQAALAEAQTLATRHQHQAVDVEHLAVALLEQEGG
                     LVPRLFERIQISPDLLRAKLEEELGRLPRVSGGTAPGHGVYVTQRLNKALVHAQDEAR
                     KLKDEYVSVEHLVLALFAEPESSGIGRIFKALGVTRGDFLKALTEVRGNQRVTSANPE
                     ATYEALEKYGRDLTRLAQQNKLDPVIGRDNEIRRCVQVLSRRTKNNPVLIGEPGVGKT
                     AIVEGLARRIVAGDIPDSLKEKRIVALDLGALIAGAKFRGEFEERLKAVLQEVAKAQG
                     RVILFIDEIHTVVGAGKAEGAMDAGNMLKPMLARGELHCIGATTLDEYRKHIEKDAAL
                     ERRFQPVMVAEPTVEDTISILRGLRERYELHHGVRIQDGALVAAAQLSQRYITDRFLP
                     DKAIDLIDEAAAKLRTEMESMPEELEGLQRRLMQLEIEREALRKEKDQASKDRLAALE
                     KEIAEVKSQRDTFSAQWEREKNALGGIQKLREAIEVARNEMEKAQRQGDLTRVAEFKY
                     GTLPGLERQLKDLETKIADAARGPRLLQEEVTPDEVAEVVSRWTGVPVARLLEGERDK
                     LLRLDQILHQRVIGQDEAVQAVADAVIRARSGLKDPKRPIGSFVFLGPTGVGKTELAR
                     ALAESLFDSEENVVRLDMSEYMEKHAVSRMIGAPPGYVGYEEGGQLTEAVRRRPYCVV
                     LFDEIEKAHHDVFNMFLQILDDGRLTDSQGRTVDFRNTIIIMTSNLGSPLLIENAGES
                     GEIQEGIRREVMAELRRHFRPEFLNRIDDTVLFKPLTLEDIKKIVELQLALLRARLAD
                     RRIELDLSDAAKEHLAREGYDPVYGARPLKRFLQRAIETPLSRQIIAGSIADRSRVRV
                     EFKKGELVFESKADGA"
     gene            complement(5161..6177)
                     /locus_tag="PKK20_07655"
     CDS             complement(5161..6177)
                     /locus_tag="PKK20_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNU99801.1"
                     /translation="MAIQYKDYYETLGVPRGASEADIKSAFRRLARQHHPDVAKDKRG
                     AEERFKEINEAYEVLGNPDNRKKYDQLGANWRAGTEFRPPPGWRSGGRGADGAGGFGG
                     GSTFEFRFGGTGFSDFFEQFFGRSGRFRQGGGFGDFEPDGFEGESESASSAHRGADVE
                     GSILVTLEEVLRGSVRSISMRWTNPHTGRSESRAIKVRIPPGVGEGQLIRVAGRGETG
                     IGGGGAGDLYLRVRLAAHPDFRIQGHDLYHELDLAPWEAVLGATVSVPTLDGQVSLRI
                     RPGAASGQQLRVRGHGLPRRADGRRGDLYVVLTIQVPGEVSASEKALWQQLARESRFD
                     PRKK"
     gene            6495..>6769
                     /locus_tag="PKK20_07660"
     CDS             6495..>6769
                     /locus_tag="PKK20_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNU99802.1"
                     /translation="METGSYNPFQTAQSQFDKVASLLDLDEGIRDLLRQPMREYQFTI
                     PVHMDDGKVKVFKGFRVQHNDARGPAKGGIRFHPMETIDTVRALAMW"
BASE COUNT         1181 a         2125 c         2137 g         1326 t
ORIGIN      
        1 ttttcgtcaa gcttccaacc atttttttcg ccaattcctg tgggatggct gtgttccaaa
       61 gtcgcggttt gtggacaatg aggaattcga ggccgttgag atgggaatac gtctccgcaa
      121 tcttcatggg gtgccgctcc tcccgtagct tcgctttttc tcgagaagaa ccatctgcgc
      181 ggtgtcaccc tcagccgtcc aaggggcggc ggtcaagctg ttgcccgcct caaccgggtc
      241 atacaccggc ttgtgcatcg gacgcgtgcg gagggtgcct gcgagctcgc cttctatcct
      301 ctctcttgcc aatgacacgt acttcggcac cgtctcggct ccggcagccc gacgaccgtg
      361 gcgcaccgct gcgacgatgg ttgtcccggt tccgaggaag ggatccaaca cccagtcgtc
      421 ttcctcggcc agcgaaagca ccagccgttc gaccaactcg actgggaatt ggcatggatg
      481 ctcggtcttt tcgacatggt tgctcttcac attcggaatg acccagaggt cccctgggtt
      541 ctttcccaac gggttgcatg aaaactgtcc cgcttttggc cccttgaaat atttctttcc
      601 agggtacttc tgtggaacac gaactgggtc aaggttgaac acatagtcat ccgacttcgt
      661 gaaccaactg atcgtttcgt agcggccgga aaatctcctg gaacagtgca agccatgctc
      721 aaaatgccag atgatgcggt tgcgcatact caatccaagc tctctgaaga tcggataaag
      781 cacagtgtcc agtggaatga tagcccctcc gtccacatag ttgccaactt gccagcaaat
      841 gctgcctcga ggcgaaagac atcgaacaca ctcccgaatc acctcggcct gttggctcag
      901 gtaactgtca agttccagct tcttttcgta ctccttcccg atattgtagg ggggggaggt
      961 cacgatcaag cggaaggtct catccgggca cgttctcaag agttcaaggc agtctcccgg
     1021 aaacacaact atccgctcgt ccttgctgaa acagtcgccg attcggccgc gcttcgccaa
     1081 catgcaacga caatacctgg tcgtcacggc acggtcaatg tcggtatgtc gggcgacgaa
     1141 ccacggcagc atccctgcag agcgaacgcc caggtgagcc acggcgaccg cagtctgtca
     1201 tacgagcggt ggcgaattcg gccaacgcca agcgcagcca cgccgggcca gaggcgttcg
     1261 tttgaaaccg agacgctctc gaggcattgg ctgctgcgtc tcgttagcgc gcatttcagg
     1321 tgaaatgtga cggtgtccgg tcagatgaca tcgacagttg gtgagaaatg cgggctaatg
     1381 atgggcggtc ttgtgcatca cgagcagttc gcgcacagcg cggcgtggag acggcggcgt
     1441 ctcttcgccg tagcccaggc gcagcaggat ttggggatag ccggccaggc ccaacatgct
     1501 cgccagttgg gcgcgcaact cggggatttc gatcggttga ttcaggaacg aggcccagag
     1561 gttctcggag cgggcatgga gcagcaggct ctgcaaagcc tggcccgtgt tgagccaggt
     1621 tggggcgttg tcctcaggcg tccagagcac ggccagcacc ggcgagtggg tggcaagctc
     1681 ccggtcgcgt gcggcctgtc cgccaccgcg atcgaacgtg cggatcagcg ccggcccggc
     1741 gaaggacatc cagtccttga ttcccaggtc gtggaccggg agtccgtccc tgtggctttc
     1801 ggccttggtt cggacccatc gggctagctc ttcccgaaag gccttgtccg cccactgcat
     1861 gcggtcggcc tgggcgacga gatcggcgac cgcgttgcgg acttcctcga cagcgtaggc
     1921 ggagagccag gcgccttccg cctgcgtgag cgactcgagt tccgccagca actcggtcgg
     1981 caccggatcg gggcggaagg cctggcggtt ggtgcgtcgt tgggtgatgg cgtgaaacac
     2041 gagcacgtcg tccgaagagg tctcgccgct caatcccaac cgcaattggg ccaggaggtc
     2101 cggaagattc ggattcggga acagggagac gtcgtacttg cgaccgaaat actccgccgc
     2161 tatccgcagg ttgtacaagg cggcgccgca actgatggtg agttcgcgat cgcagggatc
     2221 gatcacccgg agggcgcgtc ggcggtcggc aaacagctcg acgtccatcg cgttgatcct
     2281 gaagagccag ggctgggtgt tgtggctcga aggggcgaga atcgcgtaat tgagcagaaa
     2341 ctcgagctgg tcggaggcat atccgctctc cgggaagtcg tctgcgttca cgctccaagg
     2401 ctggagatgg tctttcatag gagtgtcacc agcattagac gcccatttcc cgagtagttt
     2461 caatccaaac ctctgtcggc ctcaggcgcg ccctaacccg gatgtttcac aaagtttcgc
     2521 ggagtctgaa acatccgggc taagccccgt ccgccttgga ctcgaagacg agctcgcctt
     2581 tcttgaactc gacccgcacg cggctgcgat cggcgatcga gccggcgatg atctggcggg
     2641 agagcggggt ttcgatcgcg cgttgcagga aacgtttgag cgggcgcgca ccatacaccg
     2701 ggtcgtaccc ttcgcgagcg aggtgttcct tggcggcgtc gcttaggtcc agctcgatgc
     2761 ggcgatccgc cagccgggcg cgaaggagcg caagctggag ttcgacgatc ttcttgatgt
     2821 cctcgagggt gagcggcttg aacaggacgg tgtcgtcgat acggttgaga aactcggggc
     2881 ggaaatggcg gcggagttcg gccatgacct cgcgccggat gccttcctgg atttcgcccg
     2941 actcgcccgc gttctcgatg agaagcgggc tgccgaggtt cgaagtcatg atgatgatcg
     3001 tgtttcggaa atcgacggtg cggccctggc tgtcggtgag ccgtccgtca tcgagaatct
     3061 gcaggaacat gttgaagacg tcgtggtgtg ccttttcgat ttcgtcgaac aggacgacgc
     3121 aatagggtcg gcggcgcacg gcctcggtca gttggccgcc ctcctcgtag ccgacatagc
     3181 cgggcggggc gccgatcatg cgggacacgg cgtgtttttc catgtactcg ctcatgtcca
     3241 gacgcacgac attctcttcg ctgtcgaaga gcgactcggc cagcgcacgg gccagctcgg
     3301 tcttccccac gccagtcggc cccagaaaaa cgaacgagcc gatgggccgc ttcgggtcct
     3361 tcagtcccga tcgggcgcgg atgacggcgt cggccacggc ctgcacggct tcgtcctggc
     3421 caatcacgcg ctggtgcaga atctggtcga gacgcagcag cttgtcccgc tccccttcga
     3481 gcaggcgcgc aaccggaacg ccggtccagc gtgacacgac ctcggcgacc tcgtcgggcg
     3541 tgacttcttc ctggagcagg cgggggccgc gcgcggcgtc ggcgatcttg gtctcgaggt
     3601 ccttgagttg ccgctccagg cccggcagcg tgccgtactt gaactcggcc acgcgcgtca
     3661 ggtcgccctg gcgctgggct ttctccattt cgttgcgggc gacctcgatg gcctcgcgga
     3721 gcttctgaat gccgcccagg gcattcttct cgcgctccca ttgggcgctg aaggtgtcgc
     3781 gctggctttt gacctcggca atttcctttt cgagggcggc cagacggtcc ttcgaggcct
     3841 ggtccttctc cttgcgcaag gcctcccgct cgatctcgag ttgcatgagg cggcgctgga
     3901 gcccttcgag ttcctcgggc atcgactcca tctcggtccg cagtttggcc gcggcttcat
     3961 cgatgaggtc gatggccttg tcgggcagga aacggtccgt gatgtagcgc tgggacagtt
     4021 gcgcggcagc aacaagggcg ccgtcctgga tgcggacgcc gtggtggagt tcgtagcgtt
     4081 cgcgcaggcc gcgcaggatg ctgatcgtgt cctcgacggt cggctcggcg accatcacgg
     4141 gctggaaacg gcgttcgagc gcggcgtctt tctcgatatg ctttcggtat tcgtcgagcg
     4201 tcgtggcccc aatgcagtgc agttcgccac gggccagcat cggcttgagc atgttgccgg
     4261 catccatcgc gccctcggcc ttcccggcgc caacgaccgt gtgaatctcg tcaatgaaga
     4321 ggatgacgcg gccctgggct ttggcaacct cctggagaac ggccttgagg cgctcctcga
     4381 attcgccgcg gaacttggcc ccggcgatca gcgcgccgag gtccagggcc acaatacgct
     4441 tctccttgag gctgtccggg atgtctccag ccacgatgcg gcgggccagg ccctcgacaa
     4501 tggccgtctt gcccacgccg ggttcaccga tcaacaccgg gttgtttttg gtgcggcggg
     4561 acaggacctg aacgcagcgg cgaatctcgt tgtcgcgccc gatcaccgga tcgagcttgt
     4621 tctgctgggc gaggcgggtg agatcgcgcc cgtatttctc gagggcctcg tacgtcgcct
     4681 ccgggttggc actggtcacg cgttgattgc cccgcacctc ggtcagggcc ttgagaaaat
     4741 ccccacgcgt cacgcccagc gccttgaaga tacgtccgat cccgctcgac tccggctcgg
     4801 cgaacagggc aagcaccagg tgctcgacgc tcacgtactc gtccttgagc ttgcgcgctt
     4861 cgtcctgggc gtgaaccaga gccttgttca ggcgctgggt gacatagacg ccgtggccgg
     4921 gcgccgtgcc cccgctgacg cgcggcaacc ggcccagttc ctcttcgagt ttcgcccgca
     4981 gaagatcggg cgagatctga atgcgctcga agagccgggg cacaaggccg ccctcctgtt
     5041 cgagcagggc cacagccaaa tgctcgacgt cgaccgcttg gtgttgatgc cgcgttgcca
     5101 gggtctgggc ctcggccagg gcggcttggg atttctcggt gaatttgttc aggtccatgg
     5161 tcatttcttt ctaggatcaa atcgggattc acgggccaac tgctgccata gggctttttc
     5221 ggacgcgctg acctcgcccg gcacctggat cgtgaggaca acatacaggt cgccgcgtct
     5281 gccgtccgcg cggcgcggca gcccgtggcc gcgcacgcgc agttgctgtc cggaggcggc
     5341 gccgggccgg atgcggagcg acacctgtcc gtcgagggtg ggaaccgaca cggtcgcgcc
     5401 gagcacagcc tcccacggag ccaggtcgag ttcgtgatag agatcgtgtc cctggatgcg
     5461 aaaatcgggg tgcgcggcca accgcacccg caggtagaga tcgccggccc cgccgccgcc
     5521 aatgccggtc tcgccacggc cggccacgcg aatgagctga ccctcgccca cacccggcgg
     5581 aatccgcacc ttgatggccc gcgactccga cctgcccgtg tgcgggttgg tccagcgcat
     5641 cgagatggaa cggaccgacc cgcgcagcac ttcctcgagc gtgacgagga tgcttccctc
     5701 gacatccgcg ccgcgatgag ccgaagaagc ggattcggac tcgccctcga aaccgtccgg
     5761 ctcgaaatcg ccgaacccgc cgccctgacg gaaccgcccc gaccggccga agaactgctc
     5821 gaagaaatcg ctgaagcccg tgcctccaaa acggaactcg aacgttgagc cgcctccgaa
     5881 gccgccagcc ccgtcagccc cgcgtccgcc ggaccgccat ccgggcggcg gacgaaactc
     5941 cgtcccggcg cgccaattgg cgcccagttg gtcatacttc ttgcggttgt ccgggttgcc
     6001 gaggacttcg taggcctcgt tgatctcctt gaaccgctcc tcggcgcccc gcttgtcctt
     6061 ggccacatcc ggatggtgct ggcgcgccag gcgtcggaac gcgctcttga tgtccgcttc
     6121 gctcgcgccg cgcggaacac ccagagtttc gtagtagtct ttgtattgga ttgccatcga
     6181 ctgcgccgcc gcgacggttc gcacaccggg gctcgccggc gcacacggtc catgcacccg
     6241 ccctgatccg cgcagaggcg gccataagca tagcgaatcc gcggcggatt tccagtcgga
     6301 ctgagccgtt atgcccgcat cgatcacagg ccgcgcgctg acactagcct ccgctcgatc
     6361 aatggctcca ttcaactgag cacgcccttt cccgtttttg ttgttggcaa cggtttgttg
     6421 acacgcagga tcgcgcgcag taatgaagcg ctgtgctgtc cgaccacagt ccgtcgaaag
     6481 aaaggcaagg atcaatggag acaggttcgt ataatccgtt ccaaaccgca cagtcccagt
     6541 tcgacaaggt cgcctccctt ctggacctgg acgaggggat cagggatctg ttgcgacagc
     6601 ctatgcggga gtaccagttc accatcccgg tccacatgga cgacgggaag gtcaaggtct
     6661 tcaagggctt tcgcgtgcaa cacaacgatg cgaggggtcc ggccaagggg ggcatccggt
     6721 ttcacccgat ggagaccata gacaccgtgc gggccctggc catgtggat
//
LOCUS       DAOQRO010000172         6735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11195_length_6735_cov_6.561826, whole genome shotgun sequence.
ACCESSION   DAOQRO010000172 DAOQRO010000000
VERSION     DAOQRO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11195_length_6735_cov_6.561826"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1554
                     /locus_tag="PKK20_07665"
     CDS             178..1554
                     /locus_tag="PKK20_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99803.1"
                     /translation="MNTSNPTSPSIESTCSRRHFLQGSSIALAGAALAEFPFVLAARA
                     EADMPIRIGVIGCGGRGTGAVLDALGAATQVIYPSAGYHTEDVASNATVAHKNLEVVA
                     LCDVFMDRVNACRQNLKKLGVNVPDAMCFDGFDGYKKLLEIPEINYVIHATLPHFRPL
                     HVKAAVEAGKNVFMEKPGAVDGPGVRTLLEAGEIAKAKNLGIAAGTQRRHRPSYNEAI
                     RRIHDGQIGEIQCLRCYWNGGVIWVIEKTDAMSDMEWQLRNWNYFTWTSGDHIVEQHV
                     HNLDIMNWAMGAHPVKAYGQGGRQARRHPIHGNIYDHFAVEFEYPNGVRMFSQCRQMD
                     GTEGRVGEVIQATQGVATLNDGNNLLTIRGGGNWRSRGLEDGNPYQKEHQNLIASIRA
                     GKPINEAKSLAESTLTAIMGRESAYTGRTVEWDEALHSKLRYGPDTYELGSLPFPEVP
                     VPGKHKFA"
     gene            1824..2834
                     /locus_tag="PKK20_07670"
     CDS             1824..2834
                     /locus_tag="PKK20_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNU99804.1"
                     /translation="MTRQLHSTLLRVRPLACLCAAWALLLALGTGCEPREPREADGST
                     QDSKPRIALIMKSLANEFFLTMENGARAHQKSHATEYDLLANGIKDELDVARQIDLVE
                     QMIAQRVAAIVIAPADSKALVTVCRKAQDAGIIVVNIDNKFDDTVLAEKQARIPFVGP
                     DNRKGARRVGDYLARRLKPGDPVAIIEGPPNAFNAQQRKLGFTEAVQAAGLRLASSQS
                     GYWETDKANQVAAAILTEHPDLKAFLCSNDSMALGAIAALRAAGKADTVLVVGYDNIS
                     AVQALLKEGRILATADQHADQIAVYGIEYALLLLKTKSAPADRETPVDLVTAQGLEPP
                     AP"
     gene            2843..4396
                     /locus_tag="PKK20_07675"
     CDS             2843..4396
                     /locus_tag="PKK20_07675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNU99805.1"
                     /translation="MPDTSPLLEVRALGKRYAAPVLVDVDFEVRRGEVHALVGENGAG
                     KSTLSRIIAGLTLPDAGSMILEGAPYAPRSKREAERSGVRLVMQELNLIGNLTVAENI
                     FLDELPHRWGWIRYRALHDRARAVLDPLGLGALHPGRRVSALGIGQQQMIEIAAGLSQ
                     RCSLLILDEPTAALMDAEIEHLFAQIKRLKAAGTGILYISHRLEEIQRISDRVSVLRD
                     GQMIATRPTREISLDEIVRQMVGRELEQLGKRRPAAPGPLALRVTGLRRKPVVKDVSF
                     EVRRGEILGFAGLMGSGRTETMRAVFGADAPDAGEIYVGSSTQPARLRSPKEAIAQGL
                     ALLTEDRKGQGLLLPWSIRANLSLLCLPEISGLAGWIRRAAEHDQAGRWARALGVRCH
                     SLEQRVAELSGGNQQKVVIAKWLARNCDILIFDEPTRGIDVGARRDIYRLITNLADHG
                     KAVILVSSDLRELLALCDRIAVMSAGRIAATFDRGSWSQDRIMAAALSGYLGGNALAP
                     ASEKTAADA"
     gene            4396..5388
                     /locus_tag="PKK20_07680"
     CDS             4396..5388
                     /locus_tag="PKK20_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU99806.1"
                     /translation="MKPPYPPTPTSSRAAEWRQVLAEYLGLTVALVILMAVFSLSAQH
                     FFSAITLRTIANQIPSALLVATAMTYVLVIGEIDLSVGSVLALSGAVMGVAMAQWHWP
                     LWAALIAALATGLVCGAFNGLVSIRWRLPSFIVTLGTLEMARGGAHAVTRSQTQYIGS
                     AVGAIADTDILGFSLPFLVAVMMVVVGQLVLARTVFGRHMVAVGTNQEAVRLSGIDPR
                     PIKLAVFLITGTLVALAAAIDTSRFQSANPNAGLGFELQAIAAVVIGGTSLMGGRGSV
                     VSTFLGVLIIAVLNSGLASVGAKDEMKRLVTGFVIVAAVIVDHYRHRLTKARTV"
     gene            5391..6308
                     /gene="rbsK"
                     /locus_tag="PKK20_07685"
     CDS             5391..6308
                     /gene="rbsK"
                     /locus_tag="PKK20_07685"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNU99807.1"
                     /translation="MKPRIVVVGSSNTDMIIRLNRLPRPGETLLGGEFSMAAGGKGAN
                     QAVAAARAGGHVTFIARVGKDVFGDRALEGFRRDGIAVDRIIRDPRSASGVALIFVGA
                     EGQNSIAVASGANARLTASDVRKARRVIAEADLLMMQLETPLATVRAAAQEAAAARVS
                     VVLNPAPARPLSRTLLRLVTVLTPNETEAEMLSGVRIRNDRSAVRAAVALLGQGPKIV
                     AITLGARGALVATREGHQIVPAFPVRAVDTTAAGDVFNGALAVALAEGRELNDAVRFA
                     NAAAALSVTRLGAQPSAPKRSAIERLLCG"
     gene            complement(6476..>6735)
                     /locus_tag="PKK20_07690"
     CDS             complement(6476..>6735)
                     /locus_tag="PKK20_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HNU99808.1"
                     /translation="QIENAIRPTAMGKKAFLFIGHPDAGWRSAVIYSIMGSCRRYRID
                     PAKYLKDVLTRLPHLKQRDIPSLTPRESAKAHPEARTLPPK"
BASE COUNT         1184 a         2317 c         2110 g         1124 t
ORIGIN      
        1 tcctgatgcg cgcccaggag gaagcctcgc gccgctatgg ggtgcccttt ccgcccagcc
       61 cgcccatacc cggcttgaat tcgccgccga tgcctggcac cgagtgaccg gccatgggcc
      121 agtctggttg ttgtttcttc tggcttttcc ccgagcgcct cctccactat ttcgcgcatg
      181 aatacctcga atccaacttc accctcgatc gaatcgacat gctcccgacg ccatttcctg
      241 caggggtcat cgatcgcctt ggccggcgcg gcactcgccg agttcccgtt cgtgcttgcc
      301 gcccgcgcgg aggcggacat gccgatcagg atcggggtga ttggctgcgg gggacgcggg
      361 acgggcgcgg tgcttgatgc cctgggcgcg gccacccaag tcatctaccc gagcgcgggc
      421 tatcacaccg aagacgtggc gtcgaacgcg acggtcgccc acaagaacct cgaggtggtc
      481 gccttgtgcg acgtgttcat ggaccgcgtc aacgcctgcc gccagaatct gaagaagctc
      541 ggcgtgaacg tccctgacgc gatgtgcttc gacgggttcg atggctacaa gaagctgctc
      601 gagattcccg aaatcaacta tgtgatccac gccaccctgc cccatttccg cccgctccat
      661 gtcaaagcgg ccgtcgaggc cggcaagaac gtcttcatgg aaaaacccgg cgccgtcgac
      721 ggccccggcg tgcggacctt gctcgaagcg ggggaaatcg ccaaggcgaa gaacctcggc
      781 attgcggccg gcacccagcg tcggcaccgc cccagctaca acgaggcgat ccgccgcatc
      841 cacgacgggc agattggcga gattcaatgc ctgcgttgtt attggaacgg cggcgtgatc
      901 tgggtgatcg agaagacgga tgcgatgagc gacatggaat ggcagctccg caactggaac
      961 tacttcacct ggaccagcgg ggaccacatc gtcgagcagc atgtgcataa cctcgacatc
     1021 atgaactggg ccatgggcgc ccaccccgtc aaggcctacg gccagggagg acgccaggcg
     1081 cgccggcacc cgatccacgg caatatctac gaccacttcg ccgtcgagtt cgagtatccc
     1141 aacggcgtgc gcatgttcag tcagtgccgc cagatggacg gcaccgaagg gcgggtgggc
     1201 gaggtgatcc aggccaccca aggcgtggcg acgctcaacg acggcaacaa tctcttgact
     1261 atccgcggcg gcggcaactg gcgcagtcgc gggctcgagg acggcaatcc ttaccagaaa
     1321 gagcatcaga atcttatcgc cagcatccgc gcgggcaaac cgatcaacga agccaaaagc
     1381 ctggccgaaa gcaccctgac ggccatcatg ggacgcgagt cggcctacac gggccggact
     1441 gtcgagtggg atgaagcgct tcactccaag ctgcggtatg gccccgatac gtacgagttg
     1501 ggcagcctgc ccttcccgga ggtccccgtc ccgggcaagc acaagttcgc ctagcgaggc
     1561 gcacctcaga ccaaatccca tccccaacag catgaaccag tctctctgtt ttcccgccct
     1621 catctcccgc gccccgctcc ttagaatcct tgaccccttc aacgcagcac agaaactcga
     1681 cttgtctgca acatgtttca tcggtcaagg atgctagccc gcatgtttca cactccgtcg
     1741 gtgaacaggg cggccagact tcgcaagagg actgctatgc gggctcgagg gcccgcctca
     1801 gaccaagccc agactccacg ctcatgacaa gacaactcca ttccactctg ctccgcgtcc
     1861 ggccgttggc ctgcctgtgc gcggcctggg ccctgctcct cgccctgggc accggctgcg
     1921 aaccccgcga accccgcgaa gccgacggct cgacgcagga ctcgaaaccc cgcatcgcgc
     1981 tgatcatgaa atcgctggcg aacgagttct tcctgacgat ggagaacggc gcccgcgccc
     2041 atcaaaagag ccatgcgacc gagtacgatc ttctggccaa cggcatcaaa gatgaactgg
     2101 acgtcgcccg ccagatcgac ctcgtcgagc agatgatcgc ccagcgcgtc gccgccatcg
     2161 tcatcgcccc cgccgactcg aaagcgctcg tcaccgtctg ccgcaaggcc caggacgccg
     2221 gtatcatcgt ggtcaatatc gacaacaaat tcgacgacac cgtcctcgcc gagaaacagg
     2281 cccgcattcc gtttgtcggt ccggacaacc gcaaaggcgc gcggcgcgtg ggagattatc
     2341 tggcccgccg gctcaaaccc ggcgatccgg tggcgattat cgaaggaccg cccaacgcct
     2401 tcaacgccca gcaacgcaag ctcggcttca ccgaggccgt ccaggccgcc ggcctgcgcc
     2461 tggccagttc gcaatccggc tattgggaaa cagacaaggc caatcaggtc gccgccgcca
     2521 ttctgaccga gcatccggac ttgaaggcgt ttctctgctc gaacgacagc atggctctcg
     2581 gggcgatcgc ggccctgcgc gcggcgggca aagcggacac ggtgctcgtc gtcggatacg
     2641 acaacatctc ggccgtccag gcgctgctca aggaaggcag gattctcgca accgccgacc
     2701 agcacgccga tcaaatcgcc gtctacggaa tcgagtacgc cctgctactg ctcaagacca
     2761 agagcgcgcc cgccgaccgc gaaacgccag tcgacctcgt caccgcgcaa ggcctcgagc
     2821 ccccggcgcc ctgatccgcc ccatgccgga caccagcccc ctgctcgagg tccgcgccct
     2881 cggcaaacgc tacgccgcgc cggtgttggt cgacgtcgat ttcgaggtgc gccggggcga
     2941 ggtgcatgca ctggtcgggg agaacggcgc gggcaagagc accctgtcgc gcatcatcgc
     3001 cggtctcacg ctgccggacg ccggctcgat gatcctcgag ggcgctccct atgccccgcg
     3061 atccaaacgt gaggccgagc gcagcggggt gcgcctggtc atgcaggagc tgaacctcat
     3121 cggcaacctc accgtcgccg agaacatctt cctcgacgaa ctgcctcacc gatggggctg
     3181 gattcggtac cgcgccctgc atgatcgggc ccgggcggtg ctcgaccccc tcgggctcgg
     3241 cgccctccat ccgggccggc gcgtcagcgc gctgggcatc ggccagcagc agatgatcga
     3301 gatcgcggcc ggcctctccc agcgctgcag cctgctcatt ctcgacgagc caaccgccgc
     3361 cctcatggac gccgagatcg agcacctctt cgcccagatc aaacgcctca aggccgccgg
     3421 caccggtatc ctctacatct cccatcgact cgaagaaatc cagcggatca gcgaccgcgt
     3481 cagcgtcctg cgcgacggtc aaatgatcgc cacacgccca acccgcgaga tttcactcga
     3541 tgaaatcgtt cgccagatgg tcggacgcga actcgaacag ttgggcaaaa ggcgcccggc
     3601 cgcccccggc cccctcgcgc tccgagtgac cggattgcgc cgcaaaccgg tcgtcaagga
     3661 tgtcagcttc gaggtccggc gcggcgaaat cctcgggttc gccggcctga tgggatccgg
     3721 acgcaccgag acgatgcggg ccgtgttcgg cgcggatgcg cccgacgccg gagaaatcta
     3781 cgtgggcagc tcgacgcaac cggcgcgcct gcgttcaccc aaagaggcga tcgcccaggg
     3841 cctggctctc ctgaccgaag accgcaaagg gcaaggcctg ctcctgccct ggtcgatccg
     3901 cgcgaatctg agcctgctct gcctccccga aatcagcggc ctggccggat ggattcggcg
     3961 ggccgccgag cacgaccagg ccggacgctg ggcgcgcgcg ctgggcgtcc gctgccattc
     4021 gctcgaacag cgggtggccg aattgagcgg aggcaaccag cagaaggtgg tcatcgccaa
     4081 atggctcgcc cgcaactgcg acatcctcat cttcgacgag ccgacccgcg gaatcgacgt
     4141 cggggcgcgc cgggacatct accgcctgat cacaaatctg gccgaccatg gcaaagccgt
     4201 catcctggtc tcctcggacc ttcgggaact cctggccctt tgcgatcgga tcgccgtcat
     4261 gtcggccggg cgcatcgcgg ccacattcga ccgcggttcg tggagccagg accggatcat
     4321 ggccgccgcc ttgagcggat acctcggggg aaacgcattg gcgcccgcct cggagaaaac
     4381 ggctgccgac gcctgatgaa gccgccttat ccaccaaccc cgacttcctc gcgcgctgcc
     4441 gaatggcgcc aggtcctggc cgagtacctg ggcctgacgg tcgccctcgt catcctgatg
     4501 gcggtgttca gcctgagcgc ccagcatttc ttcagcgcca ttaccctccg gaccatcgcc
     4561 aaccagattc cgtccgccct gctcgtggcc acggcgatga cgtatgtcct ggtgatcggc
     4621 gagattgacc tgtcggtcgg gtcggttctg gcgctctcag gcgcggtgat gggcgtggcg
     4681 atggcccagt ggcactggcc attgtgggcg gctctcatcg ccgctctggc gacaggcctg
     4741 gtctgcggcg ccttcaacgg cctggtctcg atccgctggc ggctgccctc cttcatcgtc
     4801 accctgggca cgctcgaaat ggcccgcggg ggcgcccacg ccgtgacccg ctctcagacc
     4861 cagtacatcg ggtccgccgt gggggcgatc gccgacacgg acatcctcgg tttttcactg
     4921 cccttcctcg tagcggtcat gatggtggtt gtcggacaac tggtgctcgc ccgcaccgtg
     4981 ttcggccggc acatggtggc tgtgggcacc aatcaggaag ccgtgcggct cagcggcatc
     5041 gatccgcgtc cgatcaagct tgccgtcttt ctgatcaccg gcacgctcgt ggccctggcc
     5101 gcagccatcg acacctcgcg cttccagtcg gccaatccca acgccgggct cggcttcgaa
     5161 ctgcaggcga tcgccgccgt cgtcatcggc ggcaccagcc tgatgggcgg acgcggctcc
     5221 gtcgtcagca cgttcctggg cgtgctgatc atcgccgtgc tcaattccgg gctcgcgtcc
     5281 gtgggtgcca aggacgaaat gaaacggttg gtgacgggat tcgtcatcgt ggcggccgtc
     5341 atcgtcgacc attaccggca ccgcctcaca aaagcccgga cagtctaacg atgaagccgc
     5401 gcattgttgt tgtcggcagt tcgaacacgg acatgatcat ccggttgaac cggctgccgc
     5461 gtccgggcga aaccctgctc ggcggcgagt tctcgatggc cgccggcggc aagggcgcca
     5521 accaggccgt cgccgccgcg cgcgccggcg ggcatgtgac cttcatcgcc cgcgtgggaa
     5581 aggacgtctt cggcgatcgc gccctcgaag gctttcgaag ggacggcatc gccgtggatc
     5641 gcattatccg cgatccgcga tcggcgtccg gtgtcgcgtt gatcttcgtc ggcgccgagg
     5701 ggcagaacag catcgcggtg gcctcgggcg caaacgcccg attgacggcg tctgacgttc
     5761 gcaaggcgcg gcgggttatc gccgaggcgg atttgctgat gatgcaattg gagacgccgc
     5821 tggccacggt ccgggcagcg gcccaagagg cagccgctgc tcgagtgtcc gtggtgctga
     5881 acccggcgcc ggcacggcct ttgtcgcgga cgctgctgcg cctggtgacc gtgctgacgc
     5941 ccaacgaaac cgaggccgaa atgctctccg gcgtccgcat ccgcaacgat cggtctgccg
     6001 tccgggcggc ggtcgcgctt ctcggccagg ggccgaagat cgtggccatc accctcgggg
     6061 cgcggggcgc tctggtggca acccgggaag gccatcagat cgtgccggcg tttcccgtgc
     6121 gtgcggtgga cacgaccgcg gcgggggatg tcttcaatgg ggcactcgcg gtggcattgg
     6181 ccgaggggcg ggagttgaac gatgcggtgc gctttgccaa cgccgcggcc gccttgtcgg
     6241 tgacgcgcct cggggcgcag ccctcagccc ccaaacgcag cgcgatcgag cgcctgctgt
     6301 gcggttgagg ttcgtcgacg ccgacatttc cactcgaact ggactctgtc gaggacccct
     6361 gttcgaaggt atccatccgg ggaaggggca gtaggcgcgg cgccctcgat ctgctcaagt
     6421 gagcggtgtg aactggagga cctcgcgagg accgagagcg gcgaacggag cctgcttact
     6481 tgggcggcag cgtgcgcgct tcgggatggg ctttggccga ttcccgcggt gtcagcgagg
     6541 ggatgtcgcg ttgcttgagg tggggcaagc gcgtcagcac gtccttgaga tatttggccg
     6601 ggtcgatccg atagcggcgg caactcccca tgatcgagta gatcaccgca ctacgccaac
     6661 cggcgtccgg atgcccgatg aacaggaacg ccttcttgcc catcgccgtt ggccgaatcg
     6721 cattttcaat ctgat
//
LOCUS       DAOQRO010000173         6733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11203_length_6733_cov_5.939203, whole genome shotgun sequence.
ACCESSION   DAOQRO010000173 DAOQRO010000000
VERSION     DAOQRO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11203_length_6733_cov_5.939203"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1610
                     /locus_tag="PKK20_07695"
     CDS             111..1610
                     /locus_tag="PKK20_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNU99809.1"
                     /translation="MKKSDTPQQANAKEQEQPQVEFPYEYKRPKCSVAFKIYKTPRDD
                     YDAFTLVYYQEGLRKRVLKNTFEAALAEADEVAKLLGSKDVDVLELKSADRAAYKRAR
                     EVLDPLGISIEVAAVEYAHIKRILGDVSPIVAAEYYKRKHPMEITAKRVPVVVKEFLE
                     AKEADGCSKRYLNTLKYNLGAFAKRFRGNIEPVVGGEIDAWLRTSGLSPRTRNNIRTS
                     LYTLFKFAVGRRYLPKDHDELESVSVVNDRDGDIEVFTAAELAEVLCCASERMIPFLV
                     LGAFAGIRHAEIQRLEWRDVRFDEGAIEIHASKAKTASRRLVPIVPVLKEWLLKFRQA
                     AGLVVSHRNVAFELHMIAKTANQLRRAAWAEARGVTEEQLKQAEKQARELAAKRKKSK
                     LRSQKGEVPPGAETAEIEGWEPFAWKNNGLRHSYISYRLAAVQNTAQVALEAGNSPQM
                     IFQHYRELVRPADAEKWFAVTLASVEAARAEREAGKDAKIVKLPKQAVA"
     gene            1607..2677
                     /locus_tag="PKK20_07700"
     CDS             1607..2677
                     /locus_tag="PKK20_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNU99810.1"
                     /translation="MSETIRFPMDGVGVPITGAYTVFIDESMDGFLGFPRPDGYFCYC
                     ALMVPSERLADLGCFWEALRERLVREYKKATGFALGDVEFKAGYLNQLGPTARRDLGE
                     RLAYFLRKNQCYVGGFFTTVDGFSLYHLRTKVAMDDEAKVLPDDWVARLAAVKRDLLA
                     NDEKLPGVANLIYPLLHAVLEIPLNWLGSLGKSFEAVYDPRDKREDAFLLKHVSRFLN
                     DAEAAFKRFPGIFKGATAKVKSEDSPGLMLADLLLRDIRRLFLDLPGLVLEASSEKLV
                     LPVPQNDDPLVMSFNGLRTKAGSLRPMSAELAAALRRPSANSMLPLYADRLADRKLSC
                     YASWGEARVVRFEDDRFADMVD"
     gene            complement(2871..3779)
                     /locus_tag="PKK20_07705"
     CDS             complement(2871..3779)
                     /locus_tag="PKK20_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99811.1"
                     /translation="MRFTVERKALVKMLQCVGKKAPSQKRREKQVRLSACAARVFVEA
                     NETTAGTEALVLEDGTCFLSQDIFLKVLKTYKTKPNVTVEADGRTLRFFSSTLPVTDY
                     SHAVTPPGKFQVFPVTDLSVLRPDAAPAPPVLKSPPPPEPEPPPTHRHRPGTFPITQP
                     EMASMQEMARLTRRLCSLPRIPPQEIVGLARAIHALDRLPLTTKLVDVEFSLHDYGDT
                     KESYPIVLQVSATSLSLGFILTEKSTQTNYSVTSTGNRDLEAGPDGIADWLYAFRSLL
                     DSNSKGLRLSVDDRSAPGLLSAPSKS"
     gene            complement(3935..4123)
                     /locus_tag="PKK20_07710"
     CDS             complement(3935..4123)
                     /locus_tag="PKK20_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99812.1"
                     /translation="MKVVSQMGQTAYSVAGVVYKTPTAAANSISETPINGWQFWGIES
                     TRPAGRKGRPWSVKVSKA"
     gene            complement(4236..6647)
                     /locus_tag="PKK20_07715"
     CDS             complement(4236..6647)
                     /locus_tag="PKK20_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNU99813.1"
                     /translation="MGQASSQSPGVNIFNDVLEPLFYDTLATDRGHEAWCNRFKCRIP
                     FLNGGLFEPLADYDWRKTDIILPNKLFTNTERTEAGDIGSGVLDVFDRYNFTVNEAEP
                     LEKEVAIDPEMLGKVFENLIEENRRKGLGSFYTPREIVHYMCQESLINYLDTALNPQS
                     EPIGPEKRKQALLGSEKAEQTLLTAPARRELVPRADIETLVHLGDQISHYEAVATRYA
                     IKMPKSIEQHARLIDEKLADITVCDPAVGSGAFPVGMMTEIVRARCALTTYFNDPHER
                     SPYFFKRHAIQNCLYGVDIDPGAVEIAKLRLWLSLVVDEEDVKQIKPLPNLFYKIVTG
                     NSLLGVEKTLFNKNLFQDLENLKPLYFDATDTRKKAALKREIDALIHQLTNGKETFDF
                     HIYFSEVFHQRGGFDVVVANPPYGLINKRQNKHESIIVTDQELQDFKSAPEFSPARGG
                     MINVFRLFIVRSINILRKRGCFSEIFPLAFACDSSAAALREHVLKNHSLLTVEAFPER
                     DNEKKRVFEAVKMSVCVVTLLAGQKSGAQFSLRIHKDKFVDPANEKAFLTLNTIQALD
                     STNHTIPLLKPRELAVIESVYKKASPLSTIGHCYTGEIDLTLDKHYLSHNGDNAVLLK
                     GAIIDRYRIRDSMSQGEILFLNSRQYLRENHGNRSEHHKLRRIVMQGITGVNERVRLK
                     MTLANEGTFCANSVNYIVLRNSAIQPEYLLALLNSNLLNFVFAKASTNSNVNGYEVDN
                     LPILIADGKQQEKLGTLATSILAAKQRDAEADTSTLERKIDESVYALYGLTTEEIALL
                     QSSRP"
BASE COUNT         1269 a         1654 c         2330 g         1480 t
ORIGIN      
        1 tctagtgagt aacatcgtac aggttcaagt cctgtcgcgg gcaccacttt tttccaagta
       61 ttttgagaaa aagcttgcag tgatgacaga gtgatgacac agtatcggat atgaagaaat
      121 ccgatacgcc acagcaggca aacgcgaagg agcaggagca accacaggtc gagttcccgt
      181 acgagtacaa gcggcccaag tgctcggtcg ctttcaagat ctacaaaacg ccgcgtgatg
      241 actacgacgc cttcacgctc gtttactacc aggaggggct gcgcaagcgt gtgctgaaga
      301 acacgttcga ggctgccctg gccgaggcgg atgaggtcgc aaagctgctc gggtccaagg
      361 acgttgacgt gctggaactg aagagcgcgg accgggcggc ctataagcgg gcgcgggaag
      421 tgctggaccc gctgggcatc agcatcgagg tcgcggcggt ggaatacgcg cacatcaagc
      481 ggattctcgg ggacgtgtcg ccgatcgtgg cggcggagta ttacaagcgg aagcatccga
      541 tggagattac ggccaagagg gtgccggtgg tggtgaagga gttcctggag gccaaggaag
      601 ccgacgggtg cagcaagcgc tatctgaaca cgctcaaata caacctgggc gcgttcgcca
      661 agcggtttcg ggggaacatc gagcccgtgg tgggcggcga gattgatgcg tggctcagaa
      721 cgtcggggct gtcgccgcgg acgcggaaca acatccgcac gtcgctctat acgctgttca
      781 agttcgcggt ggggcggcgg tatctgccga aggatcacga tgagctggaa tcggtctcgg
      841 tggtgaatga ccgtgacggg gacattgagg tcttcacggc ggcggagctg gccgaggtgc
      901 tttgctgcgc gagcgaacga atgattccgt tcctggtgct cggggcgttt gcgggcatcc
      961 ggcacgcgga gattcagcgg ctggagtggc gggatgtgcg gtttgatgag ggggccatcg
     1021 agattcatgc gtcgaaggcc aagaccgcga gccggcggct ggtgccgatt gtgccggtgc
     1081 tgaaggagtg gctgctcaag ttccgccagg cggcggggct ggtggtgtcg catcgcaacg
     1141 tggctttcga actccacatg attgcgaaga cggcgaacca gttgcggcgg gcggcctggg
     1201 cagaggcccg gggcgtgacc gaggagcagc tcaagcaggc cgagaagcag gccagggagc
     1261 tggccgcgaa gcggaagaag agcaagctgc gaagccagaa gggcgaggtg ccgccgggcg
     1321 ccgagacggc ggagattgag ggctgggagc cgttcgcatg gaagaacaat ggactgagac
     1381 actcgtacat cagctatcgg ctggcggcgg tccagaacac cgcgcaggtg gcgctggaag
     1441 cggggaacag cccgcagatg atcttccagc actatcgcga gctggtgcgg ccagcggacg
     1501 cggagaagtg gtttgcggtc acgttggcca gcgtagaagc agcgagggcg gagcgggagg
     1561 ccggcaagga cgcgaagatt gtgaagctgc cgaaacaggc ggtggcgtga gcgagacgat
     1621 tcgatttccg atggatggcg ttggggtgcc gatcacaggc gcctataccg tcttcattga
     1681 cgaatctatg gacgggttcc tcgggttccc gaggccggac ggctacttct gttattgcgc
     1741 gctgatggtg ccctccgagc gtcttgcgga tctgggttgc ttttgggagg cgctgagaga
     1801 gaggcttgtc cgggaataca agaaggcgac cgggtttgcg ttgggcgatg tcgagttcaa
     1861 ggcgggttac ctgaatcagt tggggcctac cgcgaggagg gacttgggcg agcggttggc
     1921 ctattttctc aggaagaatc agtgctacgt cggcggattc tttacgaccg tggacgggtt
     1981 ctcgctgtat catctgcgaa cgaaagtggc gatggacgat gaggctaagg ttctaccgga
     2041 tgattgggtc gcgcggctcg cggcggtgaa aagggatttg ttggccaatg acgaaaagct
     2101 gccaggggtc gccaatctca tctatccgtt gctgcacgcc gttctggaga ttccgctcaa
     2161 ctggctcggg agcctgggca agagttttga ggcggtctat gatccgaggg acaagcggga
     2221 ggatgcattc ctgctcaagc acgtcagcag gtttctgaat gatgctgagg ccgctttcaa
     2281 gcggttcccg gggattttca agggtgccac agcaaaggtg aagtcggagg actcgccggg
     2341 gctcatgctc gcggatctgc tgctccggga cattcgccgg ctgtttttgg atctgccggg
     2401 gttggtactt gaggcgtcga gcgagaagct ggttctcccg gtgccacaaa acgacgaccc
     2461 gctcgtcatg tcattcaacg ggttgaggac gaaggcggga agtctcagac cgatgtccgc
     2521 tgagctggct gcggcgctga gacgaccgag cgcgaactcg atgctcccgc tctacgctga
     2581 ccgtctggca gatcgcaagc tgtcgtgcta tgcgagctgg ggcgaggccc gagttgttcg
     2641 attcgaggat gatcgtttcg ctgacatggt agattagggg aacggcgccc cgcgcggtgg
     2701 gcctgcccgg ccagagggct agaggcttga cggcttcaca gcggtgggag gcggcgcggg
     2761 gctgcggtgg ctgcgcagga aacgtcggcc ggtgcggaag ccacactcta aaagcaactg
     2821 cgaccctcag aggtgcagtt gctttggctc gggtggcacc gggcttcgga ttaggatttc
     2881 gatggcgccg acaaaaggcc gggggcggaa cggtcgtcca cggagaggcg gaggcccttg
     2941 ctgtttgagt cgaggaggga cctaaaggca tatagccagt ccgcgatgcc atctgggccc
     3001 gcctccaggt cacggtttcc ggtgcttgtg acgctgtagt tcgtttgtgt gctcttttca
     3061 gtcaggataa agccgaggct gaggctggtg gctgagactt ggagcacaat ggggtaggac
     3121 tccttggtgt cgccatagtc gtggaggcta aactccacgt ccaccagttt ggtcgtgagg
     3181 ggcaggcggt cgagtgcgtg gatcgcgcga gcgaggccga cgatttcctg cggtgggatt
     3241 ctgggaaggg agcaaaggcg gcgtgtgagg cgggccatct cttgcattga ggccatctcg
     3301 ggctgggtga tggggaaggt gccagggcgg tgtcgatggg ttggcggggg ctcgggttcc
     3361 ggcggcggcg gtgactttag caccggtggc gcgggcgcgg cgtcggggcg caggactgag
     3421 aggtcggtga ccgggaagac ctggaattta ccgggcggcg tgacggcgtg agagtagtcc
     3481 gtaaccggga gcgtgctgga aaagaagcga agcgtgcggc cgtcggcttc gacggtgacg
     3541 ttgggcttgg ttttgtaggt tttcaggacc ttcaggaaga tgtcctggct gaggaagcag
     3601 gtgccgtctt cgagtacgag ggcctcggtg ccggcggtag tttcgttcgc ctcgacgaag
     3661 acgcgggcag cgcaggctga gaggcggacc tgtttttcgc ggcgtttctg ggacggggct
     3721 tttttgccaa cgcattggag catcttgacc aaggcttttc gttcgacggt gaatctcatt
     3781 tagtgaaggg gttgtgccgc aagcttgcgg tgggcggtgg ggcagcaata gcccctgcag
     3841 gtgaacgaca cgggggcggc ggtcgggtct gagttggtag ccggaggcgg cgggcgctgt
     3901 ccattggtat tggccactat gcgcccggtt cgagtcatgc cttgctcacc ttgactgacc
     3961 aggggcgtcc cttgcgaccg gctggcctcg tggactcgat gccccaaaac tgccagccgt
     4021 ttatcggggt ttccgagatc gagttggccg cggcggttgg tgtcttgtaa acgacgccgg
     4081 ccacgctgta ggccgtctga cccatctggg atacgacctt catcgtgtag gactttcctt
     4141 tgtactgctt ggtgtaggct gttcctacgg gaggcgcttt ccttactttg tgtctgcggt
     4201 ttggcataga tgtgtatttg cttggttccg gtagtttatg gtctggagga ctggaggagg
     4261 gcgatttctt ccgtggtcag gccgtagagg gcgtacaccg actcgtctat ctttcgctcc
     4321 aacgtgctcg tgtccgcctc agcatcgcgt tgcttcgccg cgaggatgct tgtagccagg
     4381 gtcccgagct tttcctgctg cttcccgtcg gcgatgagaa ttggcaaatt atcaacttcg
     4441 tacccgttga cgttgctgtt ggtgctcgcc tttgcaaaca cgaagttgag taggttgctg
     4501 ttcagcaacg cgagtaggta ttcgggctga attgctgaat tgcgtagcac gatatagttg
     4561 accgaattgg cacagaacgt tccctcgttg gccagtgtca tcttaagcct gacccgctcg
     4621 ttcacgccgg tgattccctg cattacaatt ctacgaagct tgtggtgctc cgacctgttg
     4681 ccgtgatttt cccgaaggta ttggcgcgaa ttcaaaaaaa ggatttcgcc ttggctcatg
     4741 gagtcccgaa tccggtagcg gtcaataatc gcgcccttca acagcactgc attgtcgccg
     4801 ttgtgcgata ggtaatgctt gtccagcgta aggtctatct ctcccgtgta gcagtgtcca
     4861 atagtggaga gaggcgacgc tttcttgtaa acggactcaa tcaccgcgag ctctcttggc
     4921 ttcaagagag gaatcgtgtg gttggtgctg tcgagcgcct gaatggtgtt gagcgtcagg
     4981 aacgcctttt cgttggcggg atccacgaat ttatccttgt gaattcgcag tgagaattga
     5041 gcgccggact tttggcctgc gagcagagtg actacgcaga cagacatctt taccgcttca
     5101 aataccctct tcttttcatt gtcgcgctct ggaaatgcct cgaccgtgag gagggaatgg
     5161 ttttttaaga cgtgctcgcg gagtgcggca gcactggaat cgcacgcgaa ggcgagaggg
     5221 aatatctcag agaaacagcc gcgttttctg aggatgttga tgctcctgac gatgaagagc
     5281 cggaaaacat tgatcatccc gccgcgggcg ggtgaaaatt cgggcgcaga tttgaaatct
     5341 tggagctctt gatctgtaac gataattgat tcgtgtttgt tttggcgctt gttaatcagt
     5401 ccgtagggtg ggtttgcaac gacaacgtcg aagccgcccc tctggtggaa gacttcggag
     5461 aagtaaatat ggaaatcgaa cgtctccttg ccgtttgtga gctgatgaat gagggcatca
     5521 atctcacgct ttagcgccgc cttcttacgc gtgtcagttg cgtcaaagta aagcggcttt
     5581 aggttttcaa ggtcctggaa caggtttttg ttgaataacg tcttttccac gccgagtagc
     5641 gaattgccgg tgacaatttt gtagaaaaga ttcgggagcg gtttgatttg ctttacgtcc
     5701 tcctcgtcaa ccacgaggga cagccagagg cggagcttgg cgatctcgac ggcgccgggg
     5761 tcaatgtcca cgccgtagag gcagttttgg atggcgtggc gcttgaagaa gtagggcgag
     5821 cgttcgtgag gatcgttgaa gtaggttgtc agggcgcagc gggcgcggac aatttcggtc
     5881 atcatgccca ccgggaacgc acctgaaccg accgccggat cgcagacggt gatgtcggcc
     5941 agcttttcgt caatcagccg ggcgtgttgc tcgatgcttt tgggcatctt gatggcgtag
     6001 cgtgtcgcca cggcttcgta gtgggagatt tggtcgccga ggtggacgag ggtttcgatg
     6061 tcggcgcggg ggacgagttc gcggcgcgcc ggcgcggtga gcaaggtctg ctcggccttt
     6121 tcgctgccca gcaacgcttg ctttctcttc tccggcccga tgggctcgga ttgggggttg
     6181 agggccgtgt cgaggtaatt gatgagactc tcctgacaca tgtagtggac gatttcacgc
     6241 ggggtataga acgagccgag acctttgcgg cggttttcct caatcagatt ttcaaagact
     6301 ttgccgagca tttccgggtc aatggccact tccttttcca gcggctcggc ttcattgacg
     6361 gtgaagttgt agcggtcgaa cacatcgagg acaccagagc cgatgtcgcc ggcttcggtg
     6421 cgctcggtgt tggtgaagag tttgtttggg aggatgatgt ctgtcttgcg ccagtcgtaa
     6481 tcggcgagcg gctcgaacag accgccgttc aggaacggga tgcggcattt gaaccgattg
     6541 caccacgctt cgtggccccg gtcggtcgcc agcgtgtcgt aaaagagggg ttcgaggacg
     6601 tcattgaaga tgttgacgcc gggcgactgg ctggaagcct gtcccaccaa ccgccggaga
     6661 aagtcgcgcg gaccagtacc ccccagacat tagcgtatgc tttgggggat gcactggcgg
     6721 gtgaagtttt tgg
//
LOCUS       DAOQRO010000174         6703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11297_length_6703_cov_5.842359, whole genome shotgun sequence.
ACCESSION   DAOQRO010000174 DAOQRO010000000
VERSION     DAOQRO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_6703_cov_5.842359"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK20_07720"
     CDS             complement(<1..669)
                     /locus_tag="PKK20_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HNU99814.1"
                     /translation="MSTIRLSAVDIALVVGYLVGITILGVWLGREIRGGRDFFLAGKS
                     LPWWAAGMSLVVSDIGAKDMIGLAGDGYRYGIVMMNFDFIGCIFPVLIAAFLFMPYFW
                     LAGVYTVPEYLGRRYNLGVRTFFAVIWVLFMMGTLGTIFVSAAAMFEGLLGWSFWFSV
                     MLTAVIVGLCTALGGLRAVVFTESISCIVLTAGAALICGLGLAKVDGWAHLQETIGQM
                     TWTQH"
     gene            815..1498
                     /locus_tag="PKK20_07725"
     CDS             815..1498
                     /locus_tag="PKK20_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNU99815.1"
                     /translation="MKTFAELGFPGRLIAVEGIDGSGKSTQIYLLKRWLEQQELKVFF
                     SEWNSSDLVKSATSKGKKTNLLTPTTFSLIHATDFADRYERQLVPLLRAGYLVLCDRY
                     VFTAFARDVVRGCTPEWVRGIYAFAALPDLTFLFKAQLEVSLQRILDGRPQLKYFEAG
                     MDLRLSSDPYESFRIFQGRLLEQYLAMSTEFQFHLIDANQSVEAQQSIVRRLVADRIP
                     LSDYGSPNH"
     gene            1506..2288
                     /locus_tag="PKK20_07730"
     CDS             1506..2288
                     /locus_tag="PKK20_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNU99816.1"
                     /translation="MARRPDPEPSKAHRKVRRPAPGPQVVVPQTTLKRFYGQGILGVD
                     TSRLSGKLIVMEGADGSGRSTQIARLVRWLEGRGHATVQVGLKRSTLVSEELRKAQEG
                     NVLSRTTLSLFYATDFADQLENVIIPALKSGFMVLADRYIYTLMARDLVRGMNEQWLQ
                     NLYSIALVPDAVFYLQVSPEELVQRNFSKNHALDYWESGMDLGLSRDMFDSFVQYQRL
                     MVEKFQYLQKIYGFTIVNGDRAVDDLSCELQEKVESVLIGKA"
     gene            2295..3272
                     /locus_tag="PKK20_07735"
     CDS             2295..3272
                     /locus_tag="PKK20_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNU99817.1"
                     /translation="MAETSKDFLVGVDLGGTKILAGVFDDKLNCLGRSKMSTKSQRGP
                     EAVIDRVARCVQDAIDECDLLPKQVRGVGIGAPGASDPETGRVIFAPNLVWEDVPLKK
                     DLEKLIQIPVFVENDCNVQTLGVYERELKGKPRHMVGIFLGTGIGAGLIFDGELFSGF
                     NRSAGEIGHMVLEVGGPKCGCGNRGCFEALASRTAIFRRIQAGVKDGQKTVLTEMLGG
                     DLEDMRSGDLRKALRRGDKFVEKVIEEAAEYTGIAVANIINLINPEVVVLGGGVIDAL
                     EDEMLAIIVETAHDYAMQGTDKGIAIIASKLGDDAGITGGAVLAKRGTK"
     gene            3344..4906
                     /gene="cimA"
                     /locus_tag="PKK20_07740"
     CDS             3344..4906
                     /gene="cimA"
                     /locus_tag="PKK20_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNU99818.1"
                     /translation="MKPAIEIYDTTLRDGSQGEGINFSVADKLRIAERLDQFGVHYIE
                     GGWPGSNPKDLEFFALAKRRRFRRARLAAFSMTRRKGIAVERDPSLRQLLEAETPVVT
                     IVGKTWLLHVREVLRAKPDENLAMISDTIAFFKAHGRTVIYDAEHSFDGYADEPEYAL
                     ATWRAAERAGADRVVLCDTNGGRLPREIAEVTALARRELAVPIGIHTHDDCGLGVANA
                     IAAVEAGATQVQGTINGYGERTGNCNLTSAIPILHFKLGQRTVPAGSLPKLRDLSVFV
                     DEIANVKPDGRQPWVGAAAFAHKGGMHVHAVERVVRSYEHIQPKAVGNSRRVLVSDMS
                     GRSNLLAKARELGFHLAPDAPEVRAILGRVKELESQGYEFEAAEASLALLIRGILEHN
                     PELLFTVEGYHASIRRDGANSICEAAVKVRVGDRRAHTVADGDGPVNALDGALRQGLA
                     RFFPRVGKVRLTDYKVRIIDTGTGTAAKTRVLIQSSDGAREWGTVGVSENIITASLQA
                     LVDSMEYALLRK"
     gene            complement(4848..5912)
                     /gene="pdxA"
                     /locus_tag="PKK20_07745"
     CDS             complement(4848..5912)
                     /gene="pdxA"
                     /locus_tag="PKK20_07745"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNU99819.1"
                     /translation="MSLTIGITLGDVTGIGPEVAVKALAQAAAEAPDTAFIVLGDRAW
                     LNRNNGQRLGPLPVWDPGNPRPGVGVLDPRGTELAADLQPGAIPAARAALDWLREGAT
                     RCLRGEFGALVTGPVCKESIMATGEVFVGQTEFLSALAGTDHTIMMLLGADERGRWLR
                     VALTTTHLPLSGVAAAISQARVELAIERAAEACRDLGLPRARVAVCGLNPHAGEGGRF
                     GTEEITVIRPAVAAARGRGVDVSGPWPADTLFHQAYQGECDAVVAMYHDQGLAPLKMV
                     AFDSGVNWTLGLPFIRTSPDHGTAYEIAGKDRANPSSMLAAIRLALVLARRRGPDIEA
                     GLTCAGARIPCCRPGPGGTR"
     gene            complement(5950..>6703)
                     /locus_tag="PKK20_07750"
     CDS             complement(5950..>6703)
                     /locus_tag="PKK20_07750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU99820.1"
                     /translation="QEAVEILVERKLILQEFKRAGYVLPESLVDDRVRDRLRERWGGD
                     HAAMMRELQAAGMTKERFRQRERERLIVAIMRMRNVPQDLMVSPFKIERYYADNIDKY
                     KVGDQVKLRTIMINKPKEGPADTARKRAEEILAKLSDGVAFGEMASVYSEDTFRAQGG
                     SRGWVELDREHYVQQLDEAIRALKPSEQRGPIETESAFWIVLVEDTKLNHTKPLPEVR
                     DEIEQTLLGQERVRMEKQWIDKLKARSFIRYY"
BASE COUNT         1279 a         2206 c         2059 g         1159 t
ORIGIN      
        1 atgctgtgtc caggtcatct gaccgatcgt ctcctggaga tgagcccacc cgtcgacctt
       61 ggcgagaccc aagccgcaaa tcaaagctgc gccggcggtc agcacgatgc acgagatcga
      121 ctccgtgaaa acgaccgcgc gcagaccgcc cagggccgtg cacaacccca cgataaccgc
      181 cgtgagcatc acggagaacc agaagctcca gcccagcaac ccctcgaaca tcgccgcggc
      241 gctcacgaaa attgtgccga gggtgcccat catgaaaagc acccagatga cggcgaaaaa
      301 ggtccgcacg cccaggttgt atcgccgtcc caggtactcg ggcaccgtgt aaacccccgc
      361 cagccagaaa taaggcatga acagaaaggc cgcgatcaac accgggaaaa tgcagccgat
      421 gaaatcgaag ttcatcatca cgatcccgta ccggtacccg tccccggcca ggccgatcat
      481 gtccttggcc ccgatgtccg agaccacaag cgacatgccc gcggcccacc agggcaagga
      541 cttgcccgcc aaaaagaaat cccggcctcc tcgaatctct cgacccaacc acacgcccag
      601 aatcgtgatt cccaccaggt agcccaccac cagggcgata tcgacagcgc tgagccgaat
      661 cgtggacatc gttcagaatc cttcagaccc caaatgtgga cgccagtcta cgaaacctcg
      721 cgctcgtgtg acaagactgt tgatccgatt tgccgacgta gacgaaaaag ggttgtcatc
      781 ggcagcggga cgcccttagg ttggcgcgcg attgatgaag acgttcgcgg agttgggttt
      841 cccggggcgg ctcattgcgg tcgagggcat cgacggctcc gggaaatcga cgcagattta
      901 ccttctgaag cggtggctgg aacagcagga gttgaaagtc tttttcagtg aatggaactc
      961 gtcggatctg gtcaagagcg ccacgagcaa aggcaagaag accaacctgc tcacacccac
     1021 cacgttcagc ctcatccatg cgaccgactt cgccgaccgt tacgagcgcc aactcgttcc
     1081 gctgctgcgg gccggctacc tggtcctctg cgatcgctat gtgttcaccg ccttcgcccg
     1141 cgacgtcgtg cgcggctgca cgcctgaatg ggtgcggggc atctatgcct ttgccgcgtt
     1201 gccggatctg acgtttctgt tcaaggcgca actcgaggtc tcgttgcagc gtatcctcga
     1261 tgggcgcccg caattgaagt acttcgaggc gggcatggac ctgcgtctgt cgtccgaccc
     1321 gtacgagagc ttccgcatct tccagggccg tctgcttgaa cagtacctcg cgatgagcac
     1381 tgagttccaa ttccatctca tcgacgcaaa ccagagcgtc gaggcgcaac agtcgattgt
     1441 ccggcggctt gtcgcagacc gcattcccct ctcggactac ggctctccca accactgagt
     1501 gcatcatggc gcgacgcccc gatcccgaac catcgaaggc ccatcgcaaa gtgcgacgtc
     1561 ccgcgccggg cccccaggtg gtcgttcccc aaaccactct caagcggttc tatggccagg
     1621 gcatcctggg ggtcgacacc agccggctga gcggcaagct cattgttatg gaaggggccg
     1681 atggttccgg gcgctcgacc cagatcgccc ggctggtgcg ctggctcgaa gggcgggggc
     1741 acgccaccgt gcaggtggga ttgaaacgat ccaccctcgt cagcgaggag ttgcgcaagg
     1801 cccaggaagg caacgttctg agccgcacca cgctcagcct gttctacgcc acggatttcg
     1861 ccgaccagct cgaaaacgtc atcatcccgg ccttgaagtc cggattcatg gttctggccg
     1921 accggtatat ctacacgctg atggcccgcg atctggtgcg ggggatgaat gaacagtggc
     1981 tgcagaacct ctacagcatc gccctggtcc ccgacgcggt cttttacctc caggtctctc
     2041 cggaggaatt ggtgcagcgc aacttctcca agaatcatgc cctggactac tgggaaagcg
     2101 gcatggacct gggactctcg cgcgacatgt tcgacagctt cgtgcagtac cagagactga
     2161 tggtcgagaa gttccagtac ctccagaaaa tctatggctt cacgatcgtc aacggcgacc
     2221 gggcggtcga tgacctcagc tgtgaactgc aggagaaggt ggaatccgtg ctgattggaa
     2281 aggcataaac aactatggcc gagacctcaa aggactttct tgtgggcgtt gacctgggcg
     2341 gcacgaagat tctcgccggc gtgttcgatg acaagttgaa ttgtctcggc cggagcaaga
     2401 tgagcaccaa gtcccagcgc gggcccgagg cggtcatcga ccgcgtcgcc cggtgcgtcc
     2461 aggacgccat cgacgagtgc gatctcctgc ccaaacaggt ccgtggcgtg ggcattggcg
     2521 cgcccggcgc ctcggacccc gaaacgggac gcgtcatctt cgcgcccaac ctcgtctggg
     2581 aagacgttcc gctgaagaag gacctcgaga agctgattca gatcccggtg ttcgtcgaga
     2641 atgactgcaa cgtgcagacc cttggcgtgt acgaacgcga actgaagggc aagccacgcc
     2701 acatggtcgg catcttcctg ggcacgggca tcggcgccgg cttgattttc gacggagagc
     2761 tgttttccgg tttcaatcgc agcgcgggag agatcggcca catggtgctc gaagtgggcg
     2821 gtcccaagtg cggctgcggc aatcgcggtt gcttcgaagc cctcgccagc cggaccgcca
     2881 ttttccgccg catccaggcg ggcgtcaaag acggccagaa gaccgtcctg acggagatgc
     2941 tgggcggcga tctcgaggac atgcgcagcg gcgacctccg caaggccctt cgccgcggcg
     3001 acaagtttgt cgagaaggtc atcgaagagg cggccgagta cacagggatc gccgtggcca
     3061 acatcatcaa tctcatcaac ccggaagtgg tcgttcttgg gggcggggtc atcgacgccc
     3121 tcgaagacga gatgctggcc atcatcgtcg aaaccgcgca cgattacgcg atgcagggca
     3181 ccgacaaggg catcgcgatc atcgcctcca aactcggcga cgatgccggc atcaccggcg
     3241 gcgccgtgct cgccaagcgc ggcaccaagt agctcgctct caatccgttt gcatgcccgg
     3301 tcgctttggc ctaaaaacca cgcgtccgcc ccgtcggacg cctatgaaac cggccatcga
     3361 gatctacgac acaaccttgc gcgacggcag tcagggcgag gggatcaact tctccgtcgc
     3421 cgacaagctc cgcatcgcgg aacggctcga tcaattcggc gtccactaca tcgagggcgg
     3481 ctggccgggc agcaatccca aagacctcga gttcttcgcc ttggccaagc ggcgcaggtt
     3541 ccgacgggcc cggctcgccg cgttcagcat gacccggcgc aaggggatcg ctgtcgagcg
     3601 cgatccgtcc ctgcggcaac tcctcgaagc cgagacgccc gtcgtcacca tcgttggcaa
     3661 gacatggctg ctccatgtgc gcgaggtgtt gcgcgccaag ccggatgaga acctcgccat
     3721 gatcagcgac acgatcgcgt tcttcaaggc gcacggacgg accgtgatct acgatgccga
     3781 gcattcgttc gatggctatg ccgacgagcc cgagtatgcc ctggccacct ggcgggccgc
     3841 ggagcgggcg ggagcggatc gcgtggtttt gtgcgacacc aatggcgggc gcctgccgcg
     3901 cgagatcgcc gaagtcaccg cgttggcccg ccgcgagctc gcggtcccga tcggcatcca
     3961 cacgcacgac gactgcgggt tgggcgtcgc caacgccatt gcggcggtcg aggccggagc
     4021 cacacaggtc cagggaacca tcaatgggta tggcgaacgc acgggcaact gcaatctgac
     4081 cagcgcgatc ccgatcctgc atttcaagct tgggcaacgc accgtgccgg cgggctcgct
     4141 gccgaagctg cgcgatttgt ccgtgttcgt cgacgagatt gccaacgtca aaccggacgg
     4201 gcgccagccg tgggtggggg ccgccgcctt cgcgcacaag ggcggcatgc acgtgcacgc
     4261 cgtcgagcgg gtggtgcgca gctacgaaca catccagcct aaggcggttg gcaactcgcg
     4321 gcgcgtcctg gtcagcgaca tgtcgggccg gagcaacctc ctggccaagg cgcgcgaact
     4381 cggttttcat cttgcgcccg acgcgcccga ggtgcgggct attcttgggc gggtgaagga
     4441 actcgagagc cagggatacg agttcgaggc ggcggaggcc tcgctggcgc tgctgatccg
     4501 gggcatcctc gaacacaatc ccgagctgct cttcaccgtc gagggctacc acgcctcgat
     4561 tcgcagggac ggcgccaatt cgatctgcga agccgccgtc aaggtccgcg tgggcgaccg
     4621 ccgtgcccac acggtggccg atggcgacgg gccggtgaac gcgcttgacg gggccttgcg
     4681 ccagggcctg gccaggttct tcccgcgcgt cggcaaggtg cggctgactg attacaaggt
     4741 ccggatcatc gacacgggca ccggcaccgc cgccaagacc cgcgtcctga tccagagcag
     4801 cgatggtgcg cgcgaatggg ggaccgtcgg cgtcagcgaa aacatcatca ccgcgtccct
     4861 ccaggccctg gtcgacagca tggaatacgc gctcctgcgc aagtgaggcc ggcttcgatg
     4921 tccggcccgc ggcgccgggc caggaccagc gccagccgga tggccgccag catgctcgat
     4981 ggattggccc ggtccttccc cgcgatctcg tacgcggttc catgatcggg cgaagtgcgg
     5041 atgaacggca gacccagcgt ccagttcaca ccgctgtcga aggcgaccat tttcaacggc
     5101 gcaaggccct ggtcgtggta catggccacc accgcgtcgc actcgccctg ataggcctga
     5161 tgaaacagcg tgtccgcagg ccatggaccc gacacatcca cgccgcgccc acgcgcggcc
     5221 gccacggcag gacgaatcac cgtgatctcc tcggtgccga accgaccgcc ttcgcccgcg
     5281 tggggattca aaccgcaaac ggccacccgc gcccggggca ggccgaggtc gcgacaggct
     5341 tcggcggcgc gttcgattgc cagttcgaca cgggcttgag agatagccgc cgccacgccc
     5401 gagagcggca gatgggttgt ggtcaacgcg actcgcagcc agcgcccccg ttcatcggct
     5461 cccaggagca tcatgatggt gtggtcggtg cccgccaggg ccgagagaaa ctccgtctgc
     5521 cccacaaata cctcgccggt ggccatgatc gattccttgc acaccgggcc ggtcacgagc
     5581 gccccgaact cgcctcgaag acagcgggtc gctccctccc gcagccagtc cagagccgcc
     5641 cttgcggccg ggatcgcccc gggttgcagg tccgccgcca gctccgtgcc gcgcggatct
     5701 aggaccccga cgccaggtcg cgggtttcct ggatcccaca cagggagagg gccaagccgc
     5761 tgcccattgt tgcggttcag ccaggcgcgg tcgccgagca caatgaacgc ggtgtcgggg
     5821 gcttcagccg cagcttgggc cagagccttg accgcgactt ccggtccgat gcccgtgaca
     5881 tcgcccagtg taatgccgat cgtgagtgac acgaggtcgg ggattgggat tggtccgagg
     5941 cgcgccttac tagtaatacc gaatgaagga cctggcctta agcttgtcga tccattgctt
     6001 ctccatccgg acgcgctcct ggcccagaag cgtctgctcg atctcgtccc gcacctcggg
     6061 caggggtttc gtatgattca gcttggtgtc ttcgaccagg acaatccaga aggcggactc
     6121 ggtttcgatg ggccccctct gttcactcgg tttcagcgcg cgaatggcct cgtcgagttg
     6181 ctggacatag tgctcgcgat cgagttccac ccagccccgg cttccgcctt gcgcgcgaaa
     6241 ggtgtcctcc gagtagacgc tcgccatctc gccgaaagcg acgccgtcgc tcaacttcgc
     6301 cagaatctcc tcggcacgtt tcctggccgt gtcggcaggg ccctctttcg gcttgttgat
     6361 catgatggtc cgcagcttga cctggtcacc caccttgtac ttgtcgatgt tgtccgcgta
     6421 gtacctctcg atcttgaagg gagacaccat caggtcctgg ggaacattgc gcatgcgcat
     6481 gatggcgaca atcagacgct cccgctctct ctgacggaac cgctccttgg tcatgccggc
     6541 agcctgcaac tcgcgcatca tcgccgcgtg gtcgccaccc cagcgctcgc ggaggcggtc
     6601 ccggacgcga tcgtccacca gactctcggg caggacgtat ccggctctct tgaactcctg
     6661 cagaatgagc ttgcgctcga cgagaatctc aaccgcctcc tgc
//
LOCUS       DAOQRO010000175         6654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11423_length_6654_cov_4.599485, whole genome shotgun sequence.
ACCESSION   DAOQRO010000175 DAOQRO010000000
VERSION     DAOQRO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_6654_cov_4.599485"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PKK20_07755"
     CDS             <1..937
                     /locus_tag="PKK20_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU99821.1"
                     /translation="LGIVGIGALSAWIRPAVQYLKAGAGGEAFGSPVIEALSGISMTG
                     VQAVNWACTAITGATFLLFLMLMTRKVEAAKIRPDPAEAMRRAEAQPLGQRLRHYFAE
                     GPFSNSRFLFFIFMLLPVRTLFAHQWLTFPEYILRAYDKGVADRMEWLVNWINPMIIF
                     IGVPVATALSRRADVYTMMVVGSLVSAVPAFLLCAGPNLTLLIAYFVVFSIGEALWSA
                     RFLEYASELAPPGRVAQYMGLAQIPWLLAKGTTGFYSGYVLSRFCPEGVPPAEMQTGT
                     MWLLYGSIAMMSPIGLWLARNWVRAGLRTRQIGTA"
     gene            1006..1959
                     /locus_tag="PKK20_07760"
     CDS             1006..1959
                     /locus_tag="PKK20_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNU99822.1"
                     /translation="MNRTEPKIGRTRAFEWKLPAWIVAAYALAGCSHHPHPQAVVYAA
                     QDQVYSEPILREFERRTGIRVRAVYDSEAVKTVGLANRLLAEKNHPQADLFWGNEELR
                     TRQLAAQGVFRETNGVATFGHRTRRIVINTNRLALADAPRSLTELTNPVWRGKVALGY
                     PLFGTTATHFLALRQHWGAGPWEAWCRALQANEAFVVDGNSVVVKFVGRGEAWIGLTD
                     SDDIAAGQREGYPIVALPTGPDTLFIPNTVGVIRGGPHPVEADRLLEYLQSPEALTRL
                     VEVNALEGTQIERGTGLTVDWDALLGELEAGTQTLRGLFLR"
     gene            1973..2959
                     /locus_tag="PKK20_07765"
     CDS             1973..2959
                     /locus_tag="PKK20_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNU99823.1"
                     /translation="MEGGEGAWQKGRLCAINLRVSEARQPPINASTRLCAVYGHPIAH
                     SASPPMQNAGFAALRLNWQYLAFDVRPEDLGAAISGARTMGFIGLNLTVPHKLLAMEL
                     VHCLDASARSWGAVNTIRFEAQDADGAWQPVGSFAEPPTPPVRAQGFNTDADAITRAL
                     REDLGFEPRGASVLVLGAGGAGRTAALKLAHDGVSRLFLVNRTMAKAEQARNEVLRHW
                     PRVAVALGYPEAGVDLILNATSLGLRAGDALPLDTRQFPLRRARSVYDMIYRPAETPL
                     LAAARASGCRVANGLGMLLHQGTRSLEIWTGRPAPVEAMRRALEQSLDRTDD"
     gene            2952..4046
                     /locus_tag="PKK20_07770"
     CDS             2952..4046
                     /locus_tag="PKK20_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNU99824.1"
                     /translation="MTDPARWAALPFHFWSVVFFCLGSIVGSFLNVCVYRMPRGESLV
                     SPPSHCPHCQARIPWRSNIPLASWLRQRGRCRACGAAISVRYPMIELLTALLFLGCWL
                     RYGEVSMGLPLVYCLFVAGLVVAAFIDLEHYIIPDAITLGGIGAGFVCSMIVPALHRE
                     AVLVDALRRCLVGVALGGGLIYAVMRLGKLLFGRHRLKLARGTRIYFSEEDLVLPDRL
                     ILYAEVLYRRTDAIVLEAREVELADRCYWNVRVRLTRDALQIGDTTHRPEDVPCLEVV
                     TDAVVIPREAMGLGDVKFMAAIGAFLGWAGVLFSVMASALLGSIVGLTLIALHRHARA
                     KPIPYGPYLALAAALWVFVGDRIAMWWLRM"
     gene            complement(4176..6383)
                     /locus_tag="PKK20_07775"
     CDS             complement(4176..6383)
                     /locus_tag="PKK20_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99825.1"
                     /translation="MGPRDQPPVVRSLEEGWLPVVRHEWEEDHLRVIQTCLAAPLVEP
                     IGATISPVGTETVVLAARFELSNRSEQPRTACLWLELSRTDPLQLGLDHAVFLRPHSN
                     RVDQAGVVPVRGRFDLRGRGDLELAVLEPGRAGSYHPARAGSGQPREALRYRVDLAPG
                     QSHAVDFFAPYVELLTPREAIALKGLSFDTLHAAVVRFWKERAAQGMTYDVPDRFLND
                     FFKANLWHVLISTDLDPATGRYQHGAATHVYANFLNETAMVARSLEMRSEPLEALRLL
                     VPFLANQSVKSLPGNFKTCEGVFYAAHPSEPDPYTAQGYNMHHGWALWALAEHFFWTR
                     NTNYLIQVMPQLVLAADWITRERQATRQHNPDGSRRVEFGLAPAGQLEDVEEYQYFYA
                     TDAYYHLGMQTLVDALSALRDMAASPDRALAKGPGSLSEEWWQHVVAVAARLERDTAE
                     FRQDIRASIEESVATSPVVRLRDGNFVPFVPPRAYALTHLKEGWIREALYPALHLVNG
                     RVCDPHHPFVNWMIQDLEDNLFLSQESGYGLAEPRRDFFNLGGFTLQPNLLDLALVYL
                     QRDEVANFLRAFYNTACASLYPDVSCFAEWVPQLGTGGGPLYKTPDECKFIQWMRQML
                     VFERGNTLELGLGVPRAWMRDGQRIHIDRAATYFGSLTLEVVSHAASDAVTATVAIAP
                     TVKPGGVLLRVRHPDAKPLRAAQVNGRPAQILAERQMIVLPTEATYWEINASF"
BASE COUNT         1121 a         2061 c         2227 g         1245 t
ORIGIN      
        1 cctggggatc gtggggatcg gtgccttgtc cgcctggatt cgcccggccg tgcagtatct
       61 caaggcgggt gcgggcggcg aggcgttcgg gagccccgta atcgaggcgt tgtccggaat
      121 ctcgatgacg ggcgtgcagg cggtgaattg ggcctgcacg gccatcacgg gcgcgacgtt
      181 cctgctgttt ctgatgctca tgaccagaaa ggtcgaagcg gccaagattc ggccggatcc
      241 ggcggaagcg atgcgccggg cggaagcgca gcccctgggg caacgcctgc ggcactactt
      301 cgccgaggga ccgttctcga acagccggtt ccttttcttc attttcatgc tgctgccggt
      361 gcggactctt ttcgcgcacc aatggctgac gtttcccgag tacatcctgc gcgcgtacga
      421 caagggcgtc gccgatcgca tggaatggct tgtcaattgg atcaacccga tgatcatctt
      481 catcggcgtg cctgtggcca cggcgctcag ccgacgcgcg gacgtctaca ccatgatggt
      541 ggtcggctcg ctggtcagcg ccgtccccgc gttcctgctc tgcgcggggc cgaatctgac
      601 gcttctgatc gcctatttcg tggtgttctc gatcggcgaa gccctgtggt cggcccgttt
      661 cctcgagtac gcatccgaac tggctccgcc ggggcgcgtg gcacagtaca tgggattggc
      721 ccagattccg tggctgctcg ccaaagggac caccggcttc tactccggat atgtgttgtc
      781 ccgcttctgc cccgaagggg ttcctccggc ggagatgcag accgggacaa tgtggctcct
      841 ttacgggagt attgcgatga tgtctccgat cggcctttgg ctggcgcgca attgggtgcg
      901 cgcggggttg cggacccgtc aaatcggcac tgcctgaacc caaggcgaac aatgggacgc
      961 ggctcttgcg tcagcgccgt gtcgcccgta ctctgggcca gcgtcatgaa caggaccgag
     1021 ccaaagatcg ggagaactcg agcgtttgag tggaagctgc cggcatggat cgtggccgcc
     1081 tatgcactgg ccgggtgctc gcatcatccg catccccagg ccgtggtcta cgccgcacag
     1141 gaccaggtct actcggagcc catcctgcgg gaattcgaga ggcggacggg catccgtgtg
     1201 cgcgccgtgt acgacagcga agcggtcaag acggtcggcc tggccaaccg cctcctggcc
     1261 gagaagaatc atccgcaggc agatctcttc tggggcaacg aggaactccg aacgcgccag
     1321 ttggcggctc agggagtgtt tcgggaaacc aacggtgttg ccaccttcgg acatcggact
     1381 cggcgcatcg tgatcaacac gaaccgcctt gccctggcgg atgcgccacg gtcattgacg
     1441 gaactcacca accccgtctg gcgcggcaag gtggcgctgg gctacccgct gtttggaacg
     1501 acggcaacgc atttcctcgc gttgcgtcag cactggggcg ccggcccgtg ggaggcttgg
     1561 tgccgcgccc ttcaggcgaa cgaggcgttt gttgtggacg gcaattccgt ggtcgtgaag
     1621 tttgttgggc gcggcgaggc ctggatcggg ctgaccgact cggacgacat tgccgcaggc
     1681 cagcgcgagg gctatcccat tgtcgcactg ccgacgggcc ccgacacgtt gttcattccc
     1741 aacacggtcg gggtgattcg gggagggccg cacccggtcg aagccgatag gcttctcgaa
     1801 tacctgcaaa gccccgaggc cctcacgcga ttggtcgagg tcaatgccct cgaaggcaca
     1861 cagatcgagc gggggacggg cttgaccgtg gactgggatg cgctgctggg ggaactcgaa
     1921 gccggaactc aaacactgcg tggtctgttc ctccgctagt tgacagagtc gggtggaggg
     1981 tggcgaaggc gcttggcaaa aggggcggct ctgcgctatc aacctgcgcg tgtccgaggc
     2041 ccgacaaccg cccataaacg ccagcacgcg tctctgcgcc gtgtacgggc atcccattgc
     2101 ccactcggcg tcgcccccga tgcagaatgc cggattcgcg gcgctgagat tgaactggca
     2161 gtacctcgcc ttcgatgtgc gcccggagga tctgggcgcc gcaatcagcg gggcgcgcac
     2221 gatgggcttc atcgggctga acctgaccgt tccgcacaag ttgctggcga tggagttggt
     2281 tcattgcctg gatgcgtcag cgcgctcctg gggcgccgtc aacaccatcc gattcgaggc
     2341 ccaagacgcc gacggagcgt ggcagcctgt cgggagcttc gcggaaccgc cgacaccccc
     2401 cgtccgcgcg cagggcttca acacggatgc cgatgccatc acccgcgctt tgcgggaaga
     2461 cctgggattc gagccgcgcg gcgccagtgt gcttgtgctc ggggccggcg gcgcggggcg
     2521 caccgcggcg ttgaaactgg cccacgacgg cgtgtcgcgc ctgttcctgg tgaatcggac
     2581 gatggcgaag gctgagcagg caagaaacga agtcctccga cactggccgc gcgtggcggt
     2641 ggcgctcggc tatccagagg cgggggtcga cttgatcctg aacgcgactt cactcggtct
     2701 gagggccggc gatgcattgc ctctcgacac gcggcaattc ccgttgcgcc gtgcacggtc
     2761 ggtctacgac atgatctatc gccccgcgga gaccccgctg ttggcggcgg cgagggcgtc
     2821 cggttgccga gtcgccaacg gcctgggcat gttgctccat caaggcaccc gatcactcga
     2881 gatctggacg ggccggccgg cgccggtcga ggcgatgcgt cgggctctcg agcagagtct
     2941 ggatcgcacc gatgactgac cccgcgcgtt gggcggcgct gccctttcac ttctggtcag
     3001 tcgtcttctt ctgcctgggg agcatcgtgg gcagtttcct aaatgtctgt gtgtatcgga
     3061 tgccgcgggg cgaaagcctt gtgtcgcctc cctcgcactg ccctcattgc caagcgcgca
     3121 tcccgtggcg cagcaacatc ccgctggcga gctggctccg gcagcgcgga cgctgccggg
     3181 cgtgtggcgc tgccatatcg gttcgctatc cgatgatcga gctgctgacg gccctgctgt
     3241 tcctcggctg ctggctgcgc tatggcgagg tgtcgatggg cctgcccttg gtttattgcc
     3301 tgttcgtggc cggactggtg gtcgccgcgt ttatcgacct cgagcattac atcatcccgg
     3361 atgccatcac cctgggcgga atcggcgccg gttttgtctg ttccatgatc gtgccggcat
     3421 tgcatcggga ggcagtcttg gtggacgcct tgcggcgctg cctggtcggg gttgccctgg
     3481 gcggcggcct gatctatgcc gtcatgcgcc tgggcaaact gctgttcgga cgccaccgac
     3541 tcaagctggc gcgcggcacg cgaatctact tcagcgaaga ggatcttgtg ctgccggaca
     3601 gactgattct gtacgccgaa gtcctgtatc ggcgcacgga cgcgatcgtg ctcgaggcgc
     3661 gcgaggtgga acttgcggac cgatgctact ggaacgtgcg ggtgcgattg acgcgcgacg
     3721 cgctccaaat cggcgacacg acgcatcgcc cggaagatgt gccgtgtctc gaggtggtca
     3781 ccgatgccgt ggtgattccg cgtgaggcca tgggactggg cgacgtgaag ttcatggccg
     3841 ccatcggcgc ctttctaggc tgggcgggcg ttctgttctc cgtgatggcc agcgcgctcc
     3901 tcgggtcaat cgtcggattg accctcatcg cactgcatcg tcatgcgcgg gccaagccga
     3961 ttccttacgg gccatatttg gctctggcgg cggcgctctg ggtctttgtc ggggatcgaa
     4021 ttgcgatgtg gtggctgagg atgtagaacg ccaacgcgcg ctttgcgcgg cggatgagcg
     4081 ggacggtgtc gtgcatgcaa gtgatccctc agtacatcac ggagtcttcg gcttcgcggt
     4141 ggtcgcccat tgatgaaaca tgagggccag gtggttcaga atgaggcgtt gatctcccaa
     4201 tacgtggcct cggtggggag gacgatcatc tggcgttcgg ccaggatttg cgccggtcgt
     4261 ccattgacct gagcagcccg cagcggcttg gcatccggat gccgcacgcg caagagaacg
     4321 ccgccgggct tgaccgtcgg agcgatcgcg acagtggctg tgacggcatc ggaggcggcg
     4381 tgcgaaacga cctcgagggt caggctgccg aagtaggttg ccgcgcgatc gatgtgaatg
     4441 cgctggccgt cgcgcatcca ggctcgaggc acgcccagcc ccagttcgag ggtgtttccc
     4501 cgttcgaaga cgagcatctg cctcatccat tggatgaact tgcattcgtc cggagtcttg
     4561 tagagcggac cgcccccggt tccgagctgc ggcacccact cggcgaagca ggatacgtcc
     4621 ggatacaggc ttgcgcaggc ggtgttgtag aacgcccgga gaaagttcgc cacctcgtcg
     4681 cgttggaggt agacgagcgc cagatcgagc agattgggct gcagggtgaa tccgccgagg
     4741 ttgaagaagt cccggcgtgg ttcggcaagg ccgtagccgg attcctggct aagaaacagg
     4801 ttgtcttcga ggtcctggat catccagttc acaaacggat gatgcggatc gcacactcgc
     4861 ccgttcacca ggtgcagcgc cggatacagc gcctcacgaa tccacccctc cttcagatga
     4921 gtcagcgcat aggcccgtgg aggcacgaaa ggcacaaagt tgccgtcccg cagccgcacg
     4981 accggcgatg tggcgaccga ttcctcgatg ctggcgcgaa tgtcctgacg gaactcggct
     5041 gtgtcccgct cgagccgcgc tgcgaccgcg acaacatgct gccaccattc ctcgctcaag
     5101 ctccctggtc ccttggcaag cgcacggtcc ggggaagcgg ccatatcgcg caaagccgaa
     5161 agcgcgtcca ccagcgtctg catcccgaga tggtaatagg catccgtggc atagaaatac
     5221 tgatactcct cgacgtcttc gagttggccc gcgggcgcga ggccgaactc gacccggcgt
     5281 gaaccgtccg ggttgtgctg tcgggtggct tgacgctcac gcgtaatcca gtccgccgcc
     5341 aggacgagct gcggcatcac ttgaatgagg taattggtat tgcgggtcca gaagaagtgc
     5401 tcggccaggg cccaaagcgc ccagccgtgg tgcatgttgt agccctgtgc ggtgtagggg
     5461 tccggctcgc tggggtgtgc tgcatagaag acgccctcgc acgtcttgaa attgccgggc
     5521 agactcttca cgctctggtt ggccaggaag ggcaccagga ggcgcagggc ttcaagaggc
     5581 tcgctgcgca tctcgagcga tcgggccacc atcgccgttt cgttcaggaa gttcgcatag
     5641 acatgagtcg ccgccccgtg ctgatagcgg cccgtggccg ggtccagatc ggtggaaatc
     5701 aacacgtgcc acaagttggc tttgaagaag tcgttcagga accgatccgg cacatcgtag
     5761 gtcatgccct gggcggcacg ttccttccaa aaccggacca cggctgcgtg cagcgtgtcg
     5821 aacgagaggc ccttcagggc gattgcctca cgcggagtca gcaattcgac atacggcgca
     5881 aaaaagtcga ccgcatggct ttgccccggg gcaaggtcga cccggtaccg tagcgcctcg
     5941 cggggctgtc cggaaccggc gcgggctggg tgatagctcc cggcgcgacc gggttcgagc
     6001 acggccaatt cgaggtcccc acggccacga aggtcaaaac gtccgcgaac gggaacgacg
     6061 cccgcctggt cgacccgatt cgagtgaggc cggagaaaga cggcgtgatc gaggccgagc
     6121 tgcaggggat cggtccggct cagttccagc cacagacacg cggttcgagg ttgttcggac
     6181 cggttactga gctcgaagcg agccgccagc acaacggtct cggtgccgac cggacttatc
     6241 gttgcaccaa ttggctcgac caaaggcgcc gcaagacagg tctggatgac acgcaggtga
     6301 tcctcctccc attcgtgtcg cacgaccggc agccagccct cttcgaggct ccgcacgaca
     6361 ggcggttggt cgcgcgggcc catcgccgga tgttcgccaa ccgcaaagtc gtacaccagc
     6421 gcctggtcag gccatcgacg ggcttcggcg tccggccggg tgagcgcagg ctgtcgcggc
     6481 gcatctggat ttgccctaga ggcaacagcg ccacttcctg tcgcaagccc ggcacaccga
     6541 gaaaaaggta cggcggaggc ttcgcgggca tcgccccgct cacctgttca aagctctgct
     6601 cgggacggcg cgcgacctgt tcgaccaccg tgccctcggt ccaaacctcg cccg
//
LOCUS       DAOQRO010000176         6643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11449_length_6643_cov_4.620219, whole genome shotgun sequence.
ACCESSION   DAOQRO010000176 DAOQRO010000000
VERSION     DAOQRO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11449_length_6643_cov_4.620219"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKK20_07780"
     CDS             complement(<1..229)
                     /locus_tag="PKK20_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99826.1"
                     /translation="MGANSVSNENQQANRRCFCSQRAADAYRDTIIAIPASLLLPLLG
                     KAKLKAQGGQCMSKATVFSFGAVLDEYLERDL"
     gene            complement(252..905)
                     /locus_tag="PKK20_07785"
     CDS             complement(252..905)
                     /locus_tag="PKK20_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU99827.1"
                     /translation="MNLTYYLGWRLARLGSKTYFRGRAYHAERVPTQGPIILAANHAS
                     YVDPLLIGAPLARDIHYLARASLFRHPFANWLLRSVNTVPVERDSGGGAGLKAIFERL
                     RAGAGILLFPEGTRTRDGRLQPARSGIGLVVIKSACPVVPVRVFGSFEAYGRHMRFPL
                     PKPVVVKFGLALDFVALRTEARTCSKERLKAIYQQVADEIMDAIARLEPCADRTRFP"
     gene            complement(957..1652)
                     /gene="cmk"
                     /locus_tag="PKK20_07790"
     CDS             complement(957..1652)
                     /gene="cmk"
                     /locus_tag="PKK20_07790"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNU99828.1"
                     /translation="MKAPASPIVIAIDGTSASGKSTNARLVAKALNFVYVDTGAMYRT
                     LAWYCLKKRVDINDAKAVADLCRRWKTKLICSDGQARLLVDGYCPESEIRTTETSAAV
                     PHVAAVPKVRAWMKRTQRACLQFGSLVMEGRDIGTNVFPETDYKFYLDASLEERTRRR
                     VADGVRENLAARDERDSQRAAAPLMIPLGAVIVNNSGQTPEETSAFILAEIHRRQQAR
                     RDRLGPRLNAEAT"
     gene            complement(1670..2953)
                     /locus_tag="PKK20_07795"
     CDS             complement(1670..2953)
                     /locus_tag="PKK20_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNU99829.1"
                     /translation="MPLPDLIAIEPLTGRVRAEVTVPGSKSVTNRALTLAALASGTTT
                     LRGALWSEDTRLMAEALRRLGFAIEVAPDSDESSNRTIVVEGRGGVVPSGGSVAQPLD
                     LDVGNAGTAARFLAALVCLGAGVYRLEGSPRMHERPQADLFVALRELGYRIDAPNDRL
                     PALIHGGGTRPGFCRVRIDQSSQFASALLLSARAGGWTVLVEDESADEAPYVRMTREL
                     VSLFPPRGGECAIEADASSGSYFWAVNALSADQHTQTHRVRVARWPASGWQIDERFPR
                     CIPLPLRLSRQSDLGDSIMTAIVLGPLAPHPTAFTHLGRLRVQECERVRALRAELERC
                     GAAIEEEGDTLRILPSALHGADIETYGDHRMAMCFGILGLKVPGIRIKNPACVKKSFP
                     DFFRKLAALPPKGLGATVRDARTGRVLSPGELMAE"
     gene            complement(2956..3834)
                     /locus_tag="PKK20_07800"
     CDS             complement(2956..3834)
                     /locus_tag="PKK20_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNU99830.1"
                     /translation="MLFKKVTLIGVGLLGGSLGLALRRRRLAAVVTGCVRRAVGIDEC
                     LGVGAVDEATLDLEAAVRGADLVVLCTPIGQMAQLAARFGPALKPGALVTDVGSVKGT
                     VVREVEPLVASAGGLFLGSHPMAGAEKTGVGAARADLYEGAVCVITPTGHSDNGARDK
                     IEAFWRALGSRILVLSPEQHDDLVSRSSHLVQVVAAQLVNYVLGPDRPPEQRLLCAGG
                     FRDATRIASGSPEMWRDIALANRRSLARALDELTVELHAARRLIEDGNQEALASFFAR
                     AKLRRDAWIDSNTSPE"
     gene            complement(3985..4368)
                     /locus_tag="PKK20_07805"
     CDS             complement(3985..4368)
                     /locus_tag="PKK20_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNU99831.1"
                     /translation="MSRIFDHEKLEVYQTSLAFIAWLDPVLQRLPKSLSVTDQLDRAS
                     TSIPLNIAEGNGKFTSPDRCRFFDIARGSALESAAALDVLVAKGLCSREDVLPGKDRL
                     WSIVSMLVGLIKSNSDYRVHEGSPE"
     gene            complement(4489..5418)
                     /locus_tag="PKK20_07810"
     CDS             complement(4489..5418)
                     /locus_tag="PKK20_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNU99832.1"
                     /translation="MNSMLPALRRSALLGLIVVPCLAVVTTARAAEDGVTAPGAKVER
                     LAGDFEFTEGPTADATGSVFFTDQPNDRILKWSVDGKLSVFMQPAGRANGMCFDAQGQ
                     LIACADENNELWSISPDGKKTVLLKEYEGKRLNGPNDVWVRPGGGLYFTDPFYKRSWW
                     QHTQMPQDGQHVYYLAPDGKTLSRVADDLKTPNGIVGTPDGKSLFVADIGAGKTYQFD
                     IQADGRLANKRLFCDLGSDGITLDTEGNLYLTGKGVSVFDRTGKKIEQIDVPEPWTAN
                     VCFGGADRRTLFITASKGLYAIRTRIAGTRQGK"
     gene            complement(5532..>6643)
                     /locus_tag="PKK20_07815"
     CDS             complement(5532..>6643)
                     /locus_tag="PKK20_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNU99833.1"
                     /translation="TYEDEPVDGDYTRLAGDRIGVLVPAPKNLQITVEPQDATAHGWD
                     RAIFTIDATAEAVFPPTYQWRRNGTPIPGATAKVYSLLTSASDDGAKFDCVVSLAGYP
                     TTVTSREATLKVLGDAVFVPGSLMEERFNNANRDAVNIGNVGAPNQVNYWTSFEGPVN
                     DADNYSRSVSGIFIPAVTGEYVFFTCSDDDSDLWIGTNDQPASKKLVAQQQGWSNNRE
                     WLTANGGEASMKRSDTWSPDGGMTMPYSGGIHLEAGVRYFIQGVMHEGSGGDNHSATF
                     KVGLASPDPANGDETAFVSGVIGRMESPVVELPEITSVKANSDGTLTVTWTGGGTLQY
                     TTSLTPPVSWTDATGATSPYTFTPPASTLFGRIVK"
BASE COUNT         1228 a         2079 c         2141 g         1195 t
ORIGIN      
        1 ccaaatccct ttccagatac tcgtcaagca ccgctccaaa tgaaaagacc gtggccttgc
       61 tcatgcactg gccaccctgg gccttcagtt tggccttgcc caacaaaggg agcagcagtg
      121 acgcggggat cgcgataatg gtgtcgcgat aagcatcagc agcccgttga gagcaaaagc
      181 aacgcctgtt cgcttgttgg ttttcattcg agaccgaatt tgcgcccatg aatcggtgtc
      241 ggaggggcga ttcacgggaa ccgcgtgcgg tcggcgcacg gttcgagccg ggcgatcgca
      301 tccatgattt cgtccgccac ctgctggtag atggccttga gtcgttcctt ggaacaggtc
      361 cgggcctccg tccgcagcgc aacgaaatcg agcgctaatc cgaacttcac aacgaccggc
      421 ttaggcaggg ggaaccgcat gtggcgtccg taggcctcga agctgccaaa gacacggacc
      481 ggcacgacgg gacaagcgga cttgatcacg accaggccga tcccggagcg ggccggctga
      541 aggcgtccgt cgcgggtgcg cgtcccttcc ggaaagagca gaatccccgc cccggcgcgc
      601 aatcgctcga agatggcctt gaggccggcg ccgccgccac tgtcgcgttc gacggggacc
      661 gtgttgacgg aacggagcag ccaattggcg aagggatgcc ggaacaaaga ggctcgggcc
      721 aggtaatgga tgtcgcgcgc taggggcgcg ccgatgagga gggggtcgac atacgaggcg
      781 tggttggcgg cgagaatgat cggtccctgg gttggcaccc tttcggcatg ataggcgcgt
      841 ccgcggaagt aggtctttga acccaatcgg gccaggcgcc accccaggta gtaggtcagg
      901 ttcatcgcat actcgaatgc gggctcgagt cggctcgccg ctgaacgggg cctggtttac
      961 gtcgcctcgg cattcaagcg aggaccgagc cggtcgcgcc gcgcttgttg gcgccggtgg
     1021 atttcggcca ggataaaagc gctggtttcc tcgggcgttt ggcccgagtt gttcacgatc
     1081 accgcgccca gcggaatcat gagcggcgcg gcggcgcgct ggctgtcacg ctcatcccgg
     1141 gcagccaggt tctcacgcac cccgtcggcc acccggcggc gcgtccgctc ctccaacgag
     1201 gcatcgaggt agaacttgta gtcggtttcg ggaaagacgt tcgtgccgat gtcgcggcct
     1261 tccatgacga ggctgccaaa ctggagacag gcgcgctggg tgcgcttcat ccaggcgcgc
     1321 actttgggaa cggcggcgac gtgagggacg gcggcgctgg tctcggtggt tcggatttcg
     1381 ctctcggggc aatacccgtc gaccaggagg cgcgcctggc catcgctgca gatcagcttg
     1441 gtcttccaac gccggcagag gtcggcgacg gccttggcgt cgttgatgtc cacccgcttc
     1501 ttgaggcagt accaggcgag cgtgcgatac atggccccgg tatcgacgta cacgaagttc
     1561 agggccttgg cgaccagtct ggcgttggtg ctcttgccgc tggcgctggt gccatcaatc
     1621 gcaatgacga tcggcgatgc aggggccttc acgggaacag cttcaatcct cactcggcca
     1681 tcagctctcc gggcgacaga acacgcccgg tgcgcgcatc ccggactgtt gccccgaggc
     1741 cctttggcgg aagggccgcc agcttccgaa aaaaatccgg gaatgacttc ttcacgcagg
     1801 ccggattctt gattctgatc ccggggactt tcaatcccag aatcccgaag cacatcgcca
     1861 tgcgatggtc gccgtaggtt tcgatgtcgg cgccgtggag ggcggacggc aatatgcgca
     1921 gggtgtcgcc ctcctcctcg atggccgcgc cgcagcgctc caactccgcg cgcagggcgc
     1981 gcacccgctc acattcctgg acccggagcc ggcccaaatg ggtgaaagcc gtcggatgcg
     2041 gtgcgagcgg tccgaggacg atcgcggtca tgatgctgtc gcccaggtcg ctctggcggg
     2101 acagtcggag aggcaaggga atgcagcgcg gaaagcgttc atcgatctgc catccggagg
     2161 ccggccaccg cgcgacgcgg acgcgatggg tctgggtgtg ttgatcagcg ctcagtgcgt
     2221 tgaccgccca gaaataactc ccgctcgaag cgtcggcttc gatggcgcat tcgccgccgc
     2281 gtggggggaa caggctgacg agttccctgg tcatgcggac atagggcgct tcgtcggcgc
     2341 tttcatcctc gaccagcacg gtccatcctc cggcgcgcgc gctcaggagc agggccgagg
     2401 cgaactgcga actctgatca atccgcaccc gacagaatcc cgggcgcgtc ccgccgccat
     2461 ggatcaaggc cggcagacgg tcattggggg cgtcgatgcg atagccgagt tcccgcagag
     2521 cgacgaacag gtcggcctgg ggtcgctcgt gcattcgcgg cgagccttcg agtcggtaga
     2581 ctcccgctcc gagacagacg agcgcggcca ggaaccgggc ggcggtgcct gcgttgccca
     2641 catccagatc gagcggttgc gccaccgaac cgcccgacgg caccacgcca ccgcggccct
     2701 cgacgacgat tgtgcgattg ctcgattcgt ccgaatccgg cgcgacttcg atggcgaaac
     2761 cgagccgccg cagggcctcg gccatgagcc gggtatcttc actccagaga gccccgcgca
     2821 gcgtcgtcgt cccggacgcc aacgcagcga gcgtcagggc gcgattggtg acgctcttcg
     2881 agcccgggac cgtcacctcc gcgcgcacgc gtccggtcag aggctcgatg gcgatgaggt
     2941 cgggcaatgg catggttatt caggcgaggt gttcgagtcg atccaggcgt cgcgacgcag
     3001 cttggctcgg gcgaagaaac tggccaaggc ctcctggttg ccgtcctcga tgagccggcg
     3061 cgcggcgtgc aactcgaccg tcaactcatc cagggcacgg gcgaggctcc tgcgattggc
     3121 gagggcgatg tcccgccaca tctcgggcga gccggaggcg attcgggtgg cgtcgcgaaa
     3181 accacccgcg cagagcagcc gctgctcggg aggacggtca ggtccgagga catagttgac
     3241 caactgcgca gcgaccacct gcacgaggtg actcgatcgg ctgacgagat cgtcgtgctg
     3301 ttccggcgac aacaccagga tgcgggaacc cagcgccctc caaaacgcct cgattttatc
     3361 gcgcgccccg ttgtcggaat gccccgtggg cgtgatgaca cacaccgcgc cttcgtagag
     3421 atcggctcga gcggcgccaa cccccgtctt ttccgcgccg gccatcggat ggctgccgag
     3481 gaacagaccc ccagcggacg caaccagcgg ttcgacctcc cgcacaacgg tgcctttgac
     3541 gcttccgaca tcggtgacca atgcgccggg tttcaaagcg ggtccgaagc gcgccgcgag
     3601 ttgggccatc tgaccgatgg gagtgcagag aacgaccaga tcggcaccgc gcaccgcggc
     3661 ttcgagatcg agagtcgcct cgtccaccgc accgaccccg aggcattcgt cgatgccgac
     3721 cgccctgcgc acgcagcccg tgacgaccgc ggccagccga cgacggcgca gggcgaggcc
     3781 gagcgatccg cccagcagac cgacgccaat caacgtgact tttttgaaca acactgggcc
     3841 gaggataggc aacccgccaa cggtgcgcaa agggcgaaaa acgcgagatg cgcctgtgat
     3901 gaacggatgc gtcccacatc gttcagcctt cctggcctgt cgcaatccta atcgtaatcc
     3961 taatcgtaat cgtaatcgta atctctattc cggactgcct tcgtgaaccc ggtaatccga
     4021 gttggacttg atcaagccga ccagcatcga aacgatgctc cagaggcgat ctttgccagg
     4081 cagaacgtct tctcgggaac aaaggccctt ggctaccaac acgtccaggg cggctgcact
     4141 ctccaaggcc gacccgcggg caatatcaaa gaaccggcag cggtccggac tggtgaattt
     4201 accgtttccc tccgcgatat tcagcgggat ggaggtgctg gctcggtcga gttgatcggt
     4261 caccgagagc gacttgggca gccgctgcag aaccgggtcc aaccaggcga tgaaggccag
     4321 agatgtctga tacacttcca gtttctcatg atcgaatatg cggctcatgt cgattacgat
     4381 tacgattacg attacgagaa tgtcagagcg aaaaacgcga gatgcgcctg tgatgaaccg
     4441 gcgcatccca ggctgtctgc ggcagtctcg tcagcgccct ttccctcgct atttcccctg
     4501 cctggtgccc gcgatgcggg tgcggatcgc gtagaggccc ttgctcgcgg tgatgaacag
     4561 cgtccgacga tccgccccgc cgaagcagac gttggcggtc cagggctccg gcacatcgat
     4621 ctgctcgatc ttcttacccg tgcgatcgaa gacggaaacg cctttgccgg tgagatagag
     4681 attcccttcg gtgtcgaggg taatgccgtc cgagcccaaa tcgcaaaaca accgtttgtt
     4741 cgcgagccgc ccgtcagcct gaatgtcgaa ttgataagtc tttcccgcgc caatatcggc
     4801 gacgaacagc gatttgccgt cgggcgtgcc gacaatgcca ttgggcgttt tcagatcgtc
     4861 ggccacgcgg ctcagtgtct tgccgtcggg ggccaggtaa tagacgtgct ggccgtcctg
     4921 gggcatttgg gtgtgctgcc accacgagcg cttgtagaac ggatccgtga aatagagtcc
     4981 gccgccagga cgcacccaga cgtcgttggg gccgttgagc cgcttcccct cgtattcctt
     5041 cagcagcacg gtcttcttgc cgtccgggct gatggaccag agttcgttgt tctcgtccgc
     5101 acaggcgatc aactgccctt gcgcatcgaa gcacatgccg ttggcccgac cggcgggctg
     5161 catgaacacc gacaatttgc cgtcgacgct ccacttgaga atgcgatcgt tgggttggtc
     5221 cgtgaagaac acgctgccgg tggcgtctgc ggtcgggccc tcggtgaatt cgaagtcgcc
     5281 agccaaacgc tcgactttcg caccgggcgc cgtcacgccg tcttcggcgg ccctggcagt
     5341 tgtgacaaca gcgaggcaag gaacaacgat caagcccaac agagcggaac ggcgaagcgc
     5401 aggaagcatg gaattcataa gattgggata accatacgag acacgccgcg cccgtcaatg
     5461 ctgcgaaggt ctgcgaaaaa aagaggccgg ggagaacccc cggcctctgt gactcgatgc
     5521 agacagacga gctacttcac aattcgtccg aacaatgtgc tcgccggtgg cgtaaacgtg
     5581 tacgggctgg tcgccccggt cgcgtcggtc cagctcaccg gcggggtgag agaggtcgtg
     5641 tactggagcg tcccgccacc ggtccacgtc acggtgagcg tgccgtcaga gttggctttg
     5701 acgctcgtga tctcggggag ttcgaccacc ggggattcca tgcggccgat gacgcccgag
     5761 acgaaggcgg tctcgtcgcc gttggctggg tcgggagatg cgagccccac cttgaaggtg
     5821 gcgctgtggt tgtcgccgcc cgagccttca tgcatcacgc cctggatgaa gtaccggacg
     5881 cccgcttcga gatggatgcc tccgctatag ggcattgtca tcccgccgtc gggcgaccac
     5941 gtgtccgatc gcttcatgct ggcctcgccg ccattggcgg tgagccattc acggttattg
     6001 ctccagccct gttgttgtgc gaccaacttc ttgctggccg gctgatcgtt ggtgccgatc
     6061 cagaggtcgc tgtcgtcatc cgagcaggtg aagaacacgt actcgccagt caccgccggg
     6121 atgaagatgc cgctgacgga ccgcgagtag ttgtcggcat cgttgaccgg tccctcgaag
     6181 cttgtccaat agttcacctg gttgggcgca ccgacgttgc caatattgac agcgtcgcga
     6241 ttcgcattgt tgaagcgctc ttccatgagc gagccaggga caaacacagc atcgccgagc
     6301 accttgagcg tggcttcccg gctcgtgacg gtcgtcggat aaccggccag cgagacgacg
     6361 caatcgaact tggcgccgtc atcgctcgca ctggtgagca gcgagtagac cttggcggta
     6421 gcgcccggga tcggagtgcc gtttcgccgc cactggtagg tgggtgggaa gacggcttcc
     6481 gccgtggcat cgatagtgaa gatcgcccga tcccagccgt gggccgtcgc gtcctgcggc
     6541 tcgaccgtga tctggaggtt cttgggcgcg ggaaccaaca caccgatgcg atccccggcg
     6601 agtcttgtgt agtcgccgtc cacaggttca tcctcatagg tcg
//
LOCUS       DAOQRO010000177         6619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11498_length_6619_cov_4.978062, whole genome shotgun sequence.
ACCESSION   DAOQRO010000177 DAOQRO010000000
VERSION     DAOQRO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11498_length_6619_cov_4.978062"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1011
                     /gene="lgt"
                     /locus_tag="PKK20_07820"
     CDS             235..1011
                     /gene="lgt"
                     /locus_tag="PKK20_07820"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNU99834.1"
                     /translation="MHQIALTIGKLTITWYGLLVVAGFVAGLWTASRRSLRNGISSEV
                     VADLGVWLMIGAIVGSRLWHVVVYWEEDFAGKPWWEMFAIRHGGLVFYGGLAGASLAT
                     IFYARRHQVPLWKLADVLAPSIALGHVFGRIGCLMNGCCFGLPTSLPWAIHFPPEHFT
                     QGAGVHPTQIYEAIGNLALYAALAWLYRRKGFDGRVFASYLIAYGALRFLVEFFRGDY
                     EVRYLGGWATAGHLVSLAVLGCGVGLYQWLRRSDAIAERS"
     gene            1008..2033
                     /locus_tag="PKK20_07825"
     CDS             1008..2033
                     /locus_tag="PKK20_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNU99835.1"
                     /translation="MSGSNENPEGLPRVETLVVEPALALASTRLDVYLHDQFPCVSRN
                     AIQRLIREGAIRVNERVVKPTHAPRAGDTIVVEWPAPRRATVEARELPLDILYEDRAL
                     LVLNKAAGMVVHPAAGTENDTLVNALLHHCQGQLSGIGGVARPGIVHRLDQDTSGVMV
                     VAKHDAIHLALSRQFAHRQIEKIYHAIVCGELASAAGEIDAAIARHPTHRKVMAVLAG
                     GRSARTTYRTLERLAGATWVEVRLHTGRTHQIRVHFKHLGFPIVGDAVYGRRQNLRLE
                     ELTGFAAPRQLLHARRLAFTHPESGKRVAFEAPWPADFRGALEALRGGTSADARLTWP
                     KASDTVP"
     gene            2037..3035
                     /gene="bamD"
                     /locus_tag="PKK20_07830"
     CDS             2037..3035
                     /gene="bamD"
                     /locus_tag="PKK20_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNU99836.1"
                     /translation="MKRRVVLISLIVLGLLSVPLRCPAPLVYRPGEGWTYESVGGGKW
                     VRARAKDQLEVAQEAFDNKQYGVAKKAARRTVKRWPLSDYAPQAQYLLGRVYEARRYD
                     QRAFKEYQKLIEKYPKAADYDAVLQRQYEIANRFLGGQWFKLWGFIPFFRSMEKTADM
                     FTKVVQSGPHSAIAPDAQLKIGAAREKQKEYMLAVKAYEKAADIYHNRPKIAAEAIYR
                     AGMAYRKAALTAEYDQSAAASAIATFTDLITLYPDDSRVADVRGYIRTLKTEQARGAF
                     QTARFYEKKRQWDGAKVYYNEAVSFDPESTYAIEAKRRLETVARHSRAKTASAPTP"
     gene            3032..3595
                     /gene="lptE"
                     /locus_tag="PKK20_07835"
     CDS             3032..3595
                     /gene="lptE"
                     /locus_tag="PKK20_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNU99837.1"
                     /translation="MNTRRFLGLGWCGAAILLAGCAGYRLGPTSGNDARSRSVQVNFF
                     RNEAPEPRLSEAVAHALRKQLQQEGTYTLATHNDGDIVVNGVLLAYERSPVAYQPGDV
                     TTVRDYEITLRAHVVAEERSTGRVVLDEKVRGRATVRIGARNPLPDGQMPPVLAGDQA
                     SVERQTLPILAADLARNITARLVDGAW"
     gene            complement(3846..5633)
                     /locus_tag="PKK20_07840"
     CDS             complement(3846..5633)
                     /locus_tag="PKK20_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU99838.1"
                     /translation="MNGAFIERGGKSIAVYGDPSGRRALSDIVLLSEARRDVTWAARS
                     LAEHGASIVAPEKEAELLSTPEKFWAELREKRFHDYTQQSTKVPTEPIRVSRTVKGGE
                     TLTWEGLPIRVIDTPGYTRGAVSYLVELDGRTVAFTGDLIRDDGKLQDLFSLQDAIPE
                     AKIGGYHGWAGRLGDLMASLDRLAAEKPDLLVPLRGPVIRDPTAAIARLQNRIRAVYA
                     NYLSIDALRWYFKDEHIRVKARRVLGPEAQVDWMTMAKTVPLPGSIIPISNARLILSA
                     DRSGFLVDCGSMEIIEQLRKLRAAGTLESLEHVFVTHYHDDHTDALPALVAEFGVRVH
                     ACGSLIDLIERPGDYRLPCLTRNPTPVTRKHRDGDAWRWKEYEMTILDHPGQTLHHNA
                     LLVRRDGDWSACFVGDSFTPSGIDDYCLQNRNFIHEGQGLLRCLDQLDTLPGGCLIIN
                     QHVEPAFRFSAAQVTRMREMLRQRRPLLAELLPFDDPNYGLDESWAVLHPYWVVVRPG
                     QSVELALRITNHSPCERTFRAVARAPADFTVSNAGSVLIPRHSEGHLPITVRVTPDAA
                     PGLRVIVADVAWDEAELRGWTEAVLEVVP"
     gene            6063..6497
                     /locus_tag="PKK20_07845"
     CDS             6063..6497
                     /locus_tag="PKK20_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99839.1"
                     /translation="MKPDLTSDADRFLALILLTGDGVGAMRRSPSRQSLESPEGTRAF
                     AEQRQELAELLGLSDTSDRTSKPAWPPPQTRIRTTDERVPDSAPSHTGISAGETMRIR
                     QRRESLPNETGGAGGTADREMPQGRTQREHGAPIAFGTFPVE"
BASE COUNT         1213 a         2102 c         2152 g         1152 t
ORIGIN      
        1 acgggggatg gtgatcgaga gattcctgag attgtgctcg cgcgcgccgt ggatagtgat
       61 gccgtcccgg cccgccgcga tcgtttcaga cacttgccgc acgcccaaca attagcctgc
      121 cgcgcccgcg aaatccagga tacggattgt tttgacattc ggaatcgagg ccctacagaa
      181 aagcgcgtca gcagcgtgcg gactgacgat tggcatttgc ttcgagccgc tcccatgcat
      241 caaatcgcgt tgaccatcgg taagctgacc atcacctggt acggactcct ggtcgtcgcc
      301 gggttcgtcg ccggcctgtg gacggcgagc cggcgcagcc tgcgcaacgg catttcgagc
      361 gaggtggttg ccgacctggg cgtctggctc atgataggcg ccattgtcgg ctctcgcctg
      421 tggcatgtcg tcgtctactg ggaggaggat ttcgccggca aaccgtggtg ggaaatgttt
      481 gccatacggc atggcggatt ggtgttttat ggcgggctcg caggggcgtc gctggcgacg
      541 atcttctatg cccggcgcca tcaggtgccg ctctggaagc tggccgacgt cctcgcaccc
      601 agcatcgcgc tcggccatgt gttcggacgc atcggctgtc tgatgaacgg atgctgcttc
      661 ggtctgccga cctctctgcc ctgggccatc catttcccgc ccgagcactt tacccagggc
      721 gctggggtgc acccgacgca gatctacgag gcgataggga acctcgcgct ctatgcggcg
      781 ctggcctggc tgtaccgtcg caagggattc gacggacggg ttttcgcatc gtacctgatc
      841 gcctacggcg cgctgcgatt cctggtcgag tttttccgcg gggactacga ggtccggtat
      901 ctgggcgggt gggcgactgc tggccatctg gtgagcctgg cggtgctcgg gtgcggtgtg
      961 ggcctctatc agtggttgcg aaggtccgat gccatcgccg agcgttcatg agcggctcga
     1021 acgagaatcc ggaagggctg ccgcgcgtcg agacgttggt cgtcgagccc gcgctggcgt
     1081 tggcgtcgac gcgactggat gtgtatctcc acgaccagtt cccctgcgtg tcgcgcaacg
     1141 ccatccagcg cctgattcgc gaaggggcca ttcgagttaa cgagcgcgtg gtcaaaccca
     1201 cgcatgcgcc gcgggcgggc gacacgattg tggtggaatg gccggcgccc aggcgggcga
     1261 cggtcgaggc ccgcgagttg cctctggaca tcctctatga ggaccgggcc ctgctggtgc
     1321 tcaacaaggc agccggcatg gtggtgcatc ccgccgcggg cacggagaac gacaccctgg
     1381 tcaacgccct tctgcaccat tgccagggcc agctcagcgg catcggcggc gtggcgcgtc
     1441 cgggcattgt ccacaggctg gatcaggaca ccagcggcgt catggtggtt gccaaacacg
     1501 atgccatcca tttggcgctc tcgcgccagt tcgctcatcg gcagatcgag aaaatctacc
     1561 acgccattgt ctgcggggaa ctcgcgtccg ccgccgggga gattgacgcg gcgattgccc
     1621 ggcacccgac gcaccgcaag gtgatggctg ttcttgccgg cggacgctcc gctcggacga
     1681 cgtatcgcac gctcgaacgc ctggcggggg cgacgtgggt cgaggtgcgg ctgcacacgg
     1741 ggcggacgca tcaaatccgg gtgcatttca agcatcttgg ttttccgatt gtgggcgacg
     1801 cggtctacgg caggcgccag aatctcaggc tcgaagagct gaccggcttc gccgcgccgc
     1861 gccagcttct gcacgcccga cgcctcgcct tcacccatcc ggagagcggc aaacgcgtcg
     1921 cattcgaggc gccctggccg gcggacttcc ggggcgcgct cgaagctctg cgggggggca
     1981 cgtcagccga tgcccggttg acttggccga aagcctccga tacggtcccg tgacacatga
     2041 agcgccgggt cgttctcatc tcactgatcg tgctgggttt gttgtccgtg ccgttgcgtt
     2101 gtccggcgcc actggtttac cgaccgggtg aagggtggac gtacgagtcg gtggggggcg
     2161 gcaagtgggt tcgggcgcgg gccaaagacc agctcgaggt tgcccaggag gcattcgaca
     2221 acaagcaata cggcgtcgcc aagaaggcgg cccgccgcac cgtcaagcgc tggcctttgt
     2281 cggattacgc cccccaggcc cagtatctgc tgggccgggt gtacgaggcg cggaggtacg
     2341 accagcgggc gttcaaggag taccagaagc tcatcgagaa gtacccgaaa gcggccgact
     2401 acgacgcggt gctccagcgc cagtacgaga tcgccaatcg cttcctgggc gggcagtggt
     2461 tcaagctgtg gggctttatc ccgttcttcc ggtcgatgga gaagaccgcg gacatgttca
     2521 cgaaagtggt tcagagcggg ccacacagcg cgatcgcccc cgacgcgcag ctcaagatcg
     2581 gcgctgcgcg cgagaagcag aaagagtaca tgctggccgt gaaggcgtac gaaaaagcgg
     2641 ccgacatcta ccacaaccgt cccaagatcg ccgccgaggc catctaccgc gccggcatgg
     2701 cctacaggaa ggcggccctg accgccgaat acgatcagag cgccgccgcc tcggccattg
     2761 cgaccttcac cgatctcatc acgctttatc ccgacgactc gcgtgtggcc gatgtccggg
     2821 gctacatccg cacgctcaaa accgagcagg cccgcggcgc tttccagacc gcccgctttt
     2881 acgaaaagaa gcgccaatgg gatggcgcca aggtctatta caacgaggcc gttagcttcg
     2941 atccggaatc gacctacgcg atcgaggcga agcgccggct cgaaaccgtc gcgcggcact
     3001 cccgggccaa gaccgcgtcg gcacccacgc catgaacacg cggcgcttcc ttggccttgg
     3061 ctggtgcggc gcggcgatcc tcctcgcggg gtgcgccggt taccgactgg gccccaccag
     3121 cggcaacgat gcccgatcga gaagcgtcca ggtcaacttc ttccgtaacg aagcgcccga
     3181 accgcgcctg agcgaggcgg ttgcgcatgc cttgcggaag cagctccagc aggaaggcac
     3241 ctacacgctc gccacacaca acgacggcga tattgtcgtc aacggcgtcc tgctcgccta
     3301 cgaacgctca ccggtcgctt accagcccgg cgacgtgacc accgtgcgcg actacgagat
     3361 cacgctccgc gcccacgtgg tcgccgagga gcggtccacc ggacgcgtcg ttttggatga
     3421 aaaggtccgg ggccgggcca cggtgcgcat cggcgcccgg aacccgctgc ctgacggtca
     3481 gatgcccccg gttctggccg gcgatcaggc cagcgtcgag cgtcagacgc tgcccattct
     3541 cgccgccgat ctcgcccgga atatcaccgc ccgcctcgtc gacggcgcct ggtagcgcta
     3601 gtagtcaagg gcagcagcgc aattgtgagg tatccgatct gcatgacatt ccggtccatg
     3661 acgattccag gttgaccgac agcgataggg ttagtggccc cttgtagtca gagtggccgg
     3721 acggggcgat gccaattcga tgccaattcg atgccgcatc gtgcggggag ctaacgaggt
     3781 gccaggatcg agcgggtttg tcgggaagcc ggtttgccga agacctgaaa cacgcgcttc
     3841 acccttcacg gcacgacctc gaggacggct tcggtccatc cgcgcagttc ggcctcgtcc
     3901 cacgcaacat ccgcgacgat cacccgcagg ccgggagcgg cgtccggagt cacccggacc
     3961 gtgatcggga gatggccttc cgagtgtcgc gggatgagaa ccgagcccgc gttggatacg
     4021 gtgaagtcgg ccggggcgcg cgcgacggcg cgaaaggtgc gttcgcaagg cgaatggttg
     4081 gtgatgcgca atgccagctc gacggattgg cccggacgca cgacgaccca atacggatgc
     4141 agcacggccc agctctcgtc cagaccgtaa ttcgggtcgt caaagggaag caattcggcc
     4201 agcaacggcc tgcgttgcct gagcatctcg cgcattcgtg tcacctgggc cgccgagaat
     4261 cggaacgcgg gttcgacgtg ctgattgatg atcaggcaac cgccgggcag cgtgtcgagt
     4321 tgatcgaggc agcggagcag tccctggcct tcatgaatga agttgcggtt ctgaaggcag
     4381 taatcgtcga tcccggaggg cgtgaaggaa tcgccaacga aacaggccga ccagtctccg
     4441 tcgcgtcgga ccagcaaggc gttgtggtgg agcgtttgtc cgggatgatc gagaatggtc
     4501 atctcatact ccttccaacg ccaggcgtcg ccgtcgcggt gtttgcgggt gaccggtgtt
     4561 ggattcctgg tcaaacaggg caaccgatag tcgcctgggc gttcgatcag gtcgatcagg
     4621 ctgccgcatg cgtgcacgcg cacgccaaac tcggccacca acgccggcag tgcatccgta
     4681 tggtcgtcgt gatagtgagt cacgaacacg tgctcgagag actcgagcgt cccggcggcg
     4741 cgcagcttgc gcaactgctc gatgatttcc atgctcccgc aatcgaccag gaaccccgag
     4801 cgatccgctg agagaatcag ccgcgcgttg gagatcggga tgatcgaccc gggcagcggc
     4861 acggtcttcg ccatcgtcat ccagtcaacc tgcgcctcgg gacccagcac gcggcgcgcc
     4921 ttgacgcgga tatgttcgtc tttgaaatac cagcgcagcg cgtcaatgga cagatagttg
     4981 gcgtatacgg cacgaatacg gttctgcaga cgagcgatcg ccgcagtcgg atcgcgaatg
     5041 accgggccac gcaggggcac cagcaaatcc ggcttctcgg cggccagccg gtccaggctc
     5101 gccatgagat cgcccaaacg ccctgcccag ccgtggtaac cgccaatctt ggcctccggg
     5161 atcgcgtcct gaaggctgaa gaggtcctgc aacttcccgt cgtcgcggat caggtctcca
     5221 gtgaaggcta cggttctgcc gtcgagttcg accaggtaac tcactgcgcc gcgcgtgtag
     5281 ccgggagtgt cgatcacccg gatgggaagg ccctcccacg tcagcgtctc gccgcccttc
     5341 acggtgcggc tcacccgaat cggctcggtc gggaccttcg tggattgctg ggtgtaatcg
     5401 tggaagcgtt tctcccgcaa ttctgcccag aacttctcgg gagtggacaa cagttcggct
     5461 tccttctccg gagcaacgat gctggcgccg tgttccgcca ggctgcgcgc cgcccaagtc
     5521 acgtcccgcc gcgcctcgct gagcagcacg atgtccgata gcgctcgccg gcctgacgga
     5581 tcgccgtaga cggcgatgga ctttccgccc cgctcgatga aagcgccgtt caccggaccc
     5641 gggacgacgc tgagtcctga cggaagagaa tcctcggcac gagcaacctc gggccttccg
     5701 gattcgcgcg tggcgatgcc agccgtgttg cgggcagggg tcagccgcgc tctggattca
     5761 ggcgcgtccg acggtcggac gggaggcgtc aggagcaatc cacagatcgc gagcgcgcgc
     5821 agcgcaagcc aggaagaccg gggcggagag tcttgagcgg gcatggggca atgctggcag
     5881 gaacagccac gacctgcaag ctcggcaact gttcaagaag cttctatggt ctcggaatac
     5941 acgggctccc aagctcagtg tgatcatatc cggtgacctc atccctcccg tgttggatgg
     6001 attcgaccgg aaactcgcaa taaaacggat gcgattcggc tttctctaac tggaagaaag
     6061 cgatgaaacc cgacctcaca tccgatgcgg accgcttcct ggcgctcatc ctcctgacgg
     6121 gcgacggggt cggggcgatg cgccgatcac cctcacgcca gtcgctcgaa tcgccggaag
     6181 ggactcgggc cttcgcggag caacgtcagg agttggccga attgctcggt ctcagcgaca
     6241 cgtccgatcg gacatccaaa ccggcctggc cgccaccgca gacacgaatc cgcacgactg
     6301 acgaacgggt tcccgactca gccccgagtc acaccggcat aagcgccggc gaaaccatgc
     6361 gaatccggca gcggcgggag agtctcccaa acgaaaccgg aggcgctggc ggaaccgcag
     6421 atcgagagat gccacagggc cgcacacagc gagaacacgg cgcgccgatc gcattcggca
     6481 cgtttccggt tgaatgaggg tgttatccgg attcgaagcg ctctacctcg gcccggtcaa
     6541 ccattccaat gggaaccggg cgaaaaccag gttttcgtaa ggactccgct ccccgacttc
     6601 gtagaagatg catacgacg
//
LOCUS       DAOQRO010000178         6591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11563_length_6591_cov_4.533813, whole genome shotgun sequence.
ACCESSION   DAOQRO010000178 DAOQRO010000000
VERSION     DAOQRO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11563_length_6591_cov_4.533813"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(685..1428)
                     /locus_tag="PKK20_07850"
     CDS             complement(685..1428)
                     /locus_tag="PKK20_07850"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNU99840.1"
                     /translation="MKNSLMTRREAMQRAGLAATACVAGAAQFQVAAQNSASPAAPEA
                     EPYRLPPLPYAADALEPHIDARTMEIHHGKHHAAYVQNLNRAVAEFSALAAKPVEALV
                     RDLSAIPEKIRTAVRNNGGGHVNHTLFWQLLERNKNGRPTGALARAIEDKFGSFDNFR
                     QEFTKAALGVFGSGWAWLTVDGAGLRLEATPNQDTPISFNRTPILGLDVWEHAYYLKY
                     QNRRADYIAAFFNVIHWDAVANLYNQARA"
     gene            complement(1435..2439)
                     /locus_tag="PKK20_07855"
     CDS             complement(1435..2439)
                     /locus_tag="PKK20_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNU99841.1"
                     /translation="MLNQDPAYPNFRHALLLVAVVLALQVAFAIPLAVLGVVLKSRVV
                     ESPWSLGMMNLLAIGAVLGWGCWKTGAPWPELFPLRAVPLAVWSAVAVSQAGMLIVGS
                     EIDNLVRSLLPPPSFFADLFAGLTADASGGSFFALVLVAPLTEELLFRGLILRGLLRR
                     GSVTSAIVLSALLFGLMHLNPWQSITTTMMGLLLGWWTVRTGSLLPALAAHALNNSSV
                     LWHRHLPFEVTGFNNADVMSATTQFQPLWFDAIGVALLAAGIVWLHRVQPPRNLRLAG
                     PGERGPNAPDLGVEPARSDEVPPVIDRARDLASEGPVNSPQNSFDRTGIDGSHGSRSS
                     "
     gene            2787..4262
                     /gene="der"
                     /locus_tag="PKK20_07860"
     CDS             2787..4262
                     /gene="der"
                     /locus_tag="PKK20_07860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNU99842.1"
                     /translation="MPGLIAIVGRPNVGKSALFNRIVGRRIAIVHDQPGVTRDRITAE
                     AEWRGRSFTLIDTGGLGLMRGEKADDVVVRATTEQVNLALEAAHGIILVTNVQEGVAP
                     LDREVAQHLRRAGKPILVAVNKVDEPRAEAGLPEFSVLGFERVFPISAIHGRGIEPLI
                     EAATGLLPAADPDAALATGDQAASTPLKLAVVGRPNVGKSSLVNALTRSERVIVSPMP
                     GTTRDAIDVPFEVETDGRRERYVLVDTAGIRKTRSVADSIEFFSVKRAEQSIERCDLV
                     IFVLDAEAGILEQDKKIADRIVEARKGCLVVVNKWDLFEAAVQSAQQRARAPGGAMSR
                     RGERPRSTLAQFGQWVQEKLFFLDYAPVIFTSAQSGFHLDRLLEAVRFVASQMQQRIP
                     TAILNRTLHDAIERRQPVSAAGSRMKFYYATQVKPAPPTFLLFVNRAELSTPAYEKYL
                     ADQLRRAFGYEGCPIVLIPRSRPKTVALKKRQRGRGNHRSK"
     gene            4327..6228
                     /locus_tag="PKK20_07865"
     CDS             4327..6228
                     /locus_tag="PKK20_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNU99843.1"
                     /translation="MQTPPALAPSSSTASPSRLDHRGHLPRRDRLFLLIGVILVMGAA
                     FPALAELGFRFADVDAGSLGLWEGDRPVLVYRHDLAKPEGVPADRFRSTYIHPLYGLD
                     GEVLTDDFPKDHYHHRGLFWAWPHVGVGGQETDLWMLKGIEQRFVRWLTRETRADAAL
                     LGIENGWYIGASKVMQERVWVTAHPATDTQRAIDFEFTWQPIGKPISLAGAEGKSYGG
                     LTLRFAPRTDTVITTPLGVSKDDLYMTRLAWTDLSARFAGQSASSGAAILVSPDHPDY
                     PPMWLTRHYGVLCLGWPGVESQTFPPDKAIRCGYRVLVHRGNLKHEALAQAYASYKSA
                     IEKGSRPQPVATPSPAQVSTAAPGLRAEVLDDRVRVYAGERLFTEYLFTPDGKYPFFY
                     PVMGPRTGRSVTTCRTEPYPHHSSIFFGCDRVNGGNYWQEGLERGRIASQSVRLLKAA
                     GNAIEFEQECAWQRPDAESPFTDRRRIVITAPTPDRRIIDFDITLRARGKVRIEKTNH
                     SLFSVRMAPELAVTGGGTLTNDAGDQDEKGTFGKPALWADCRGKWGGATEGVTILSHP
                     ANRWSPPPWFTRDYGFFSPTPMYWPEGGSIDLEPDETVRLRYRVLVHSDALSSDEIAA
                     LYRAWTGSQ"
BASE COUNT         1194 a         2174 c         2045 g         1178 t
ORIGIN      
        1 cttcggaaaa ccaaggggtc tggactgatt ttcaccccgc atgggatgcc agtgtagcag
       61 aatccaatgg cggcacctac cgccaagtcc ccagtcccta ctccggcagc agaggttggc
      121 ctcgcctccc ctgagcctga cgaataggat tttgccgcct cagcttccgc ttccgacttg
      181 ctggtggctt acaccattcc caggcgcggg tcgcccgcct cgcgggcata ctcccggtcg
      241 cggtgataga tgccccaacg gacctgttct tcaccatgcc tggcggttgc cttcatgatc
      301 gagtcccgat cacgccgtga ccgtgacgtt ctccgccgtc gccagcggtc tcccatcggc
      361 caggcgatcc gcgaggctcc gaagcgcagt ttcccgcgcc aatcacggtg gccatcggat
      421 agatcgccac gcaggagccc ttcttggcag cggttcatcg tcagccagag gtcgtcgcct
      481 tcgtcctccg gtcggtgcgc ttggagcagt gtctccggag cgatcggcga ctcctccacg
      541 gtcgggtacc ggagcagcaa cgcctgttgg gcaagcgtca acgattcgcg cggctccaga
      601 atacgttctc ggaaccgggc aatccgtctc cagtcaggcg ccaggaggcc tgattgtctg
      661 attcaacctt gtgaaaaatc cgggctaggc gcgcgcctgg ttgtagagat ttgccaccgc
      721 gtcccagtgg atcacgttga agaacgctgc aatgtaatcc gcacgccggt tctggtactt
      781 caggtagtac gcgtgttccc agacatcgag gccgagaatg ggcgtccggt tgaacgagat
      841 cggcgtgtcc tggttgggag tcgcttcgag gcgcagaccg gcgccgtcga cagtcagcca
      901 ggcccatccg ctcccaaaca cgcccagggc cgccttggtg aattcctggc ggaagttgtc
      961 gaaactgccg aacttgtcct cgattgctct ggcgagggcc cccgtgggcc gaccgttctt
     1021 gttcctctcg agcaactgcc agaagagggt gtgattcaca tgcccgcctc cgttgtttcg
     1081 gaccgcggtg cggattttct ccgggattgc cgacaggtcc cggacgagcg cctcgaccgg
     1141 tttggcggcc agggcggaaa actcggcgac cgcccggttg aggttctgca cgtaggccgc
     1201 atggtgtttc ccatgatgta tctccatcgt ccgggcgtcg atatgcggct cgagggcatc
     1261 cgcggcatag gggagcggcg ggagccgata cggctccgcc tcgggggcag cgggtgaagc
     1321 cgagttctgg gctgcgacct ggaattgagc agcgccggcc acgcaagccg tcgctgccag
     1381 cccggctcgt tgcatcgcct cacggcgggt cataagagag tttttcatgc gctcttagga
     1441 tgatctgctg ccatgcgagc cgtcgatgcc ggttctgtca aaggaattct gcggagaatt
     1501 gaccggtccc tcgctcgcca agtcccgggc gcggtcgatc acaggcggga cttcgtcgga
     1561 cctggccggc tcgaccccga ggtccggtgc gttcggaccg cgctcgcccg gacccgcaag
     1621 ccgaagattc cgagggggct ggacgcggtg aagccagacg attcccgcgg ccagcagcgc
     1681 cacgccgatt gcatcgaacc acaacggctg gaactgggtg gtcgccgaca tgacatcggc
     1741 gttgttgaat cctgtgacct cgaatggcag atgccgatgc cacaggacgc tcgagttgtt
     1801 caaggcgtgc gccgccaacg ccggcagcag cgaacctgtc cgcaccgtcc accacccgag
     1861 cagcagcccc atcatcgtcg tcgtgatcga ttgccagggg ttcagatgca tcagcccgaa
     1921 aaggagggcg gacaggacaa tcgcagacgt cacggagccg cgccgaagca gtccgcgcag
     1981 gatgagaccg cggaaaagga gttcctcggt cagtggggcc accagaacga gggcaaaaaa
     2041 cgagcctccg gacgcgtccg cggtgagacc ggcaaacagg tcggcaaaga aagagggtgg
     2101 gggcagcagg gagcggacga ggttgtcgat ctcggatccg acgatcagca ttcccgcctg
     2161 gctcaccgcg acggcagacc agaccgcgag cgggacggcg cgcaggggga agagttcagg
     2221 ccagggcgcg ccggttttcc aacagcccca gcccagcacc gccccgatcg ccagcaggtt
     2281 catcatgccc aggctccacg ggctctcgac cacgcgcgat ttcagcacga cgcccagcac
     2341 cgcgagcggg atcgcaaacg ccacctgtaa ggccagcaca accgccacca acagcagggc
     2401 gtgtcggaaa ttcggatagg ctgggtcctg attcaacacg tcaatcaagt cgcgatcgct
     2461 cgagattgga ctctttcccg cccccgtttc aaggaacttc ccgcgcaacc ggcgattctc
     2521 gatctgccca tcccgcagcg atgggcgcca gggcgctcag ccgcccttct atcctgtccg
     2581 tcctgtcagg gcggattctt ttgtgcgccc gccccagggc caactcatga aggcgaaaag
     2641 gccggagtgg gggacggttc gtcccctgcc atgtcaagaa taaataacct gtcactttat
     2701 ctccacttca tgaattggcg ctgcgcccgc tcggatcgtc caccttttga ctttcgcacg
     2761 gtctttcccc gggtcattct cgggacgtgc ctggtctgat cgccatcgtc ggacgtccca
     2821 atgtcggcaa gtccgctctg ttcaatcgca tcgtcggacg ccggatcgcg atcgtccacg
     2881 atcagcccgg cgtgacgcgc gaccggatca cggccgaggc cgagtggcgt gggcgttcgt
     2941 tcacgctgat cgacaccggt ggacttggcc tgatgcgggg agagaaggcc gacgacgtcg
     3001 tggtccgggc gacgaccgag caggtgaatc ttgccctgga agcggctcac ggcatcattc
     3061 tggtgacgaa tgtgcaggaa ggggtggcgc cgctcgaccg ggaggtggcc caacacctcc
     3121 gacgcgccgg caaacccatc ctggtcgccg tcaacaaggt cgacgagccg cgtgcggagg
     3181 cagggctgcc cgagttcagc gtactcgggt tcgagcgggt cttcccgatt tccgccattc
     3241 atggccgcgg gatcgaaccg ttgatcgaag cggctacggg ccttcttccc gccgccgacc
     3301 cggacgccgc cctcgcaacg ggcgatcagg ccgcgtcgac accgctcaag ctggccgttg
     3361 tcgggcgtcc gaacgtcggc aagtcctcgc tcgtcaacgc cctgacccga tccgagcggg
     3421 tgatcgtcag cccgatgccc gggacgacac gggatgcgat cgacgtgccc tttgaggtcg
     3481 agactgacgg gcggcgcgag cgttacgtgc tggtggacac ggcgggtatt cgcaagactc
     3541 gcagcgtggc tgactcgatc gagttcttca gtgtcaagcg cgccgagcag tcgatcgagc
     3601 gatgtgacct ggtcatcttc gtcctcgacg ccgaggccgg aatcctcgaa caggacaaga
     3661 agatcgccga ccgcatcgtc gaagcccgca agggctgtct tgtggtggtg aacaaatggg
     3721 acttgttcga ggcggcggtc caaagcgcgc agcagcgggc gcgcgcgccc ggcggggcca
     3781 tgagcaggcg cggggagcgt ccgcggagca cactggctca gttcggccaa tgggtgcagg
     3841 agaaactctt cttcctcgac tatgcgccgg tcatcttcac ctccgcgcag tcgggcttcc
     3901 acctggaccg cctgctcgag gccgtccgct tcgtcgcctc gcaaatgcag cagcgcatcc
     3961 ccacggccat cctgaatcgc acgcttcacg atgccatcga gcgccgccaa ccggtcagcg
     4021 cggccggatc gcgaatgaag ttctactacg caacccaggt caagccggcc ccacccacat
     4081 tcctcttgtt tgtcaatcgc gcggagctct ccacccccgc gtacgaaaag tacctggccg
     4141 atcaactgcg ccgcgcgttt ggttacgagg gatgcccgat cgtgctcatc ccgcggtccc
     4201 gccccaagac ggtcgctctc aagaagagac agcgaggtcg aggaaaccac cgatcaaaat
     4261 gacgcgcgcc gcgtctgcca tttgtttttc cacctgtcaa caccccatcc aattcacgcc
     4321 gcgcctatgc aaacaccccc agccctcgcc ccgtcttcct ccacggcctc cccatcccga
     4381 ctcgatcatc gaggccatct cccgcggcgg gatagactgt ttctcctgat cggcgtgatc
     4441 ctggtcatgg gcgccgcctt tcccgccttg gccgaattgg gcttccgttt tgccgatgtg
     4501 gatgcaggct cgcttggcct gtgggagggc gaccggcccg ttcttgtgta tcgccacgac
     4561 ctggccaagc cggagggcgt cccagccgac cgctttcgaa gcacgtatat ccacccgctt
     4621 tatggtttgg acggcgaagt gctgaccgac gattttccaa aggaccatta ccaccatcgc
     4681 ggtcttttct gggcctggcc gcatgtgggc gtgggcgggc aggagaccga cctctggatg
     4741 ctcaagggca tcgagcagcg gttcgtgcga tggctgacac gcgagacgcg cgccgacgcc
     4801 gcgctgctcg gcatcgagaa cggttggtac atcggcgcct cgaaagtcat gcaggaacgc
     4861 gtctgggtca ccgcccaccc cgccaccgac acacagcgcg caatcgactt cgagttcacc
     4921 tggcagccga tcggcaagcc gatcagcctc gccggcgcgg aaggcaagag ctacggcggt
     4981 cttaccctcc ggtttgcgcc caggaccgac acggtgatca cgaccccgct cggcgtcagc
     5041 aaggacgatc tctacatgac ccggctcgcc tggaccgatc tgagcgcgcg cttcgccggg
     5101 cagtccgcat cgagcggcgc ggcgatcctg gtttcgcccg atcatccgga ttacccgcca
     5161 atgtggttga cccggcacta cggggttctg tgcctcggtt ggccgggcgt ggaaagccag
     5221 acgttcccgc cggacaaggc catccgctgc ggctaccggg tcttggttca ccggggcaac
     5281 ctcaaacacg aggctctggc acaggcctat gcatcctata agtccgctat cgagaagggg
     5341 tcccgaccgc agcccgtcgc aaccccatcg cctgcccagg tctctacggc tgcacctggc
     5401 ctgcgggccg aggtcctgga cgaccgcgtg cgagtgtacg ccggcgagcg gttgttcacg
     5461 gagtacctgt ttacccccga tggcaagtat cccttcttct atcctgtgat ggggccgcgc
     5521 accggacgaa gcgtgaccac gtgccgcaca gagccgtatc cgcaccacag ctcgatcttc
     5581 ttcggatgcg accgtgtcaa tggcggcaac tactggcagg agggcctcga acgaggacgc
     5641 atcgcctccc agtccgtgcg gctcctcaaa gccgccggca acgccatcga gttcgaacag
     5701 gaatgcgcat ggcaacgtcc tgacgccgag tcgcccttca ccgaccgccg ccgcatcgtc
     5761 atcaccgctc ccacgccgga ccggcgcatc atcgacttcg acatcaccct gcgcgcgcgg
     5821 ggcaaggtcc gaatcgagaa aaccaaccac tcactgttct cggttcggat ggcgcccgaa
     5881 ctcgccgtga ccggcggagg gaccctgaca aacgacgcgg gcgatcaaga cgagaaggga
     5941 acattcggca agccggccct ttgggcggat tgtcggggta aatggggtgg agcgaccgag
     6001 ggcgtgacga tcctgtccca tcccgccaat cgctggtcgc cacccccctg gttcacgcgc
     6061 gattacgggt tcttctcccc gacgccaatg tactggcccg aaggcggttc gatcgacctc
     6121 gaacccgacg aaaccgtccg tctccgctac cgtgtgctgg tccattccga cgcgctgtct
     6181 tcggacgaaa tcgccgcgct ctatcgggcc tggacgggtt cgcagtagac cgatcagccg
     6241 ttaatgggat gcgcagaaga agcaagattc gggcagcaga cgccaaggag gtgggggtgg
     6301 ggaaccccgc tgaagaaacg taccgccacc cgaatcaaat cactgccctg aaagctagat
     6361 gcagacgcgt agatgtcaat accttattag gcagttttga ggatgtgagg atagccggat
     6421 cggctccgca atggtgattc tgacggcctg gtgccggatt cgagaaccgg ttcggcgagg
     6481 gattgtcact actgggtgcc catgttccat ccgcctgtcg aaccaccgca tgggtccggc
     6541 ctaggcgtgt cggacctcgg ccaggatcac cacgtagttc ttgccgtagt g
//
LOCUS       DAOQRO010000179         6588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11577_length_6588_cov_4.934946, whole genome shotgun sequence.
ACCESSION   DAOQRO010000179 DAOQRO010000000
VERSION     DAOQRO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11577_length_6588_cov_4.934946"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKK20_07870"
     CDS             <1..229
                     /locus_tag="PKK20_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNU99844.1"
                     /translation="AIAARKLQDMVNRRVQILVEGLSRRNKERLEGRTRGNHITVFEG
                     PPRLIGQLLDVRINRASAFTLYADPPIPNPN"
     gene            233..802
                     /locus_tag="PKK20_07875"
     CDS             233..802
                     /locus_tag="PKK20_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99845.1"
                     /translation="MKLWHLATLLGLAVALPQIYGLLKPGAFGDALRKFPRSEAWGLI
                     LMPLGTVWFLWNLRTDDIADFDAFKPILFIGFAVVGFGACVFVRDFLAVRGWAVVVLL
                     LAKFTLDTARWVDTPWRLVIVVWAYLWVVLALWFTISPWRMRDLIGFLTASEQRIRIG
                     SAARLAFGLLVLLLGLTAIRQAELQAAIP"
     gene            complement(864..1529)
                     /locus_tag="PKK20_07880"
     CDS             complement(864..1529)
                     /locus_tag="PKK20_07880"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNU99846.1"
                     /translation="MDHQTAPDSNRVSERHRMVQEQIAGRGRDVRNPGVLEAMRTVPR
                     HAFVPQDVAQSAYEDYPLPIGHGQTISQPYIVAFMTEQLDPQRTDRVLEVGTGSGYQA
                     AILSRLVAEVYTIEIVEPLAARAAQNLQRLGYTNVFVRAGDGYAGWPEKAPFDAIIVT
                     CAPDHVPQPLIEQLKEGGRMIIPVGEFGDQNLVLIEKQGGQIARRAVLPVRFVPMTGP
                     HAR"
     gene            complement(1630..3840)
                     /locus_tag="PKK20_07885"
     CDS             complement(1630..3840)
                     /locus_tag="PKK20_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNU99847.1"
                     /translation="MATKLLSCARLLSGCAACFAFAFAADSAAPRALPPGQLPRDIRL
                     EPLKDLDGYFPFVTPSNRAAWDGRAERVRRQLLVTLGLWPAPTRTPLNAVVHGRIERD
                     DYTVERVYFESLPGFYVTGSLYRPKSPGQAKRPAVLCPHGHWANGRFLDTGAEDVRRE
                     IAAGAERFEEAGRSPLQARCVGLARMGCVVFHYDMIGYADSVQITQALAHGFAKQRPE
                     MNTTENWGLFSPQAEAHFQSVMGLQTWNSIRALDFLTGLPDVDADRIAVTGASGGGTQ
                     TFILCAIDPRPAVAFPAVMVSTAMQGGCTCENACGLRIGTGNVEFAALFAPKPQGMTA
                     ADDWTKEMGTKGFPELKRHYALLGAPDNVMLKEALHFGHNYNSVSRMAMYGWMNRHLG
                     LGQKEPIIERDFRRLSTAELTVWNDQHPKPEGGPEFERKLLRRLTEDAARQLAEAAAS
                     RDQFERVYGGGIDAVIGRGLKDVGEVVWESSAQVDLEACHQTTGRLRNLTHGEELPAI
                     RLEPRQHKAGVVIWVSGSGKSGLYTSAGEVRSELRPLLEQGWTVLGLDVLFQGEFLAD
                     AKPITQTRRVKNPREAAAYTFGYNHSLFAQRVHDVLTAIAYAKEQWGGTGRLVLAGLE
                     GGGPWVAGACAQARGALDAVALDTGGLRFGKVLDLHSPDFLPGGALYGDLPGMIALGK
                     PRRLWLAGEGDRPPALVKAAYDADGEARAIALYSGADAEACSEVSRWLLTMARP"
     gene            3933..4637
                     /locus_tag="PKK20_07890"
     CDS             3933..4637
                     /locus_tag="PKK20_07890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99848.1"
                     /translation="MILHKLIVHHARHGYDHAFSLMQVDDAVRWLRDRGVAMGQGTRA
                     LDLGCGSGTFGQRLLEMGCRVTFADESDWLPAEIPRTCFRRINLDTESLESLGRHDLV
                     ICSNVLEHLRESERLIRSFPALLAPGGHVYLSWTNWLSPWGGHDFSPWHYLGPRLGPR
                     LFDRLVGKPRALKPFENLFVTHIGRTLRLLRAQRHLELVAVAPRYYTELWSLMKIPVL
                     REVLAWNCAALAARRD"
     gene            complement(4643..5641)
                     /gene="sppA"
                     /locus_tag="PKK20_07895"
     CDS             complement(4643..5641)
                     /gene="sppA"
                     /locus_tag="PKK20_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNU99849.1"
                     /translation="MLLLVLLSIAVASVGRWAIGLLQLSGGSTRGTSIRLDEVVMDDQ
                     GGRDKVAVIDIEGIIFGGLLGGGSSGPVEMVREQLKRAESDHAVKAVLLRIDSPGGEV
                     LASDEINRSLAEFQQRSGKPIVASMGSVAASGGYYVAAPCRWIVANPLTITGSIGVIM
                     RGYNYRGLMNKVGVRPEVYKSGRFKDMLRGDKAEEDILPEEREMVQSLVDTTFRRFKE
                     VVVEGRQSPYRLSEDGGRALVDNWEDYVDGRVFSGRQAYDWGFVDELGNFKTALDRAK
                     TLAQIDRARIIRYEQPFELGNLFRLFVRTETPAIHVDLGIEFPPIQTGRLYFLFLP"
     gene            complement(6027..>6588)
                     /locus_tag="PKK20_07900"
     CDS             complement(6027..>6588)
                     /locus_tag="PKK20_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNU99850.1"
                     /translation="RLLTEFTASESLRKHALAVEAAMRHYAAHFNEDVPLWGLVGLVH
                     DFDYERWPEPPAHTREGARLLRERGVGEEIVGAMLSHAEWNQGDYPLDRPLRKTLFAV
                     DEMCGFVMAVAYVRPERLAGMAPSSVRKKMKQKSFAAAVKREDLERGAALLEIPLDDH
                     INHVIQALQGIAPELGLTVPSPDPAA"
BASE COUNT         1107 a         2127 c         2137 g         1217 t
ORIGIN      
        1 cgcgatcgcg gcacggaagc tccaggacat ggtgaatcgc cgggtgcaga ttctcgtcga
       61 gggactgagc cgcaggaaca aggagcggct cgaaggacgc acacgcggaa accatatcac
      121 cgtctttgaa ggcccgccgc ggctcatcgg gcaactgctg gacgtgcgga tcaaccgcgc
      181 ctcggcattc accctgtacg ccgatccacc catcccgaat cccaactgac ccatgaaact
      241 ctggcatctc gcaaccctgc tcggcctggc tgtggccctg cctcaaatct atggtctgct
      301 caagcccggc gcgttcgggg acgccctgcg caagtttcca cgctcggagg cgtgggggct
      361 gatcctgatg ccactgggca cggtctggtt cctatggaac ctcaggaccg atgacatcgc
      421 cgattttgac gcattcaagc cgatcctgtt catcgggttc gccgtggtcg gtttcggggc
      481 gtgcgtcttc gtgcgcgatt tcctggccgt gcggggatgg gcggttgtgg tgctgcttct
      541 cgccaagttc accctcgaca ccgcgcgctg ggtcgacacc ccgtggcggt tggtgatcgt
      601 ggtctgggcc tatctctggg ttgtgctcgc actctggttc acgatctccc cctggcgcat
      661 gcgcgatctc attggtttcc tgacggccag cgagcagcgg atcaggattg gcagcgcggc
      721 ccggctggct ttcgggcttc ttgtcctgct gctgggcctt accgccatac ggcaggcgga
      781 gcttcaggct gcaattccgt agcccgtgat ttcacactct gcggagtctc acacccaggc
      841 tctggcgaaa ccgcgcccgg gattcagcgc gcgtgcggac cggtcatagg gacgaaccgc
      901 acaggcaaca ccgcgcggcg cgcgatctgg ccgccctgct tctcgatcag aaccagattc
      961 tgatcgccaa actcgccgac cggaatgatc atacgtccgc cctccttcag ttgttcgatc
     1021 aaaggctgcg gcacatggtc gggcgcgcat gtgacgatga tggcgtcgaa gggcgccttc
     1081 tcaggccaac cggcgtaacc atcccccgcc cggacgaaga cgttcgtata cccgagtcgt
     1141 tggagattct gcgcggcccg cgcagcgagc ggttcgacga tctcgattgt gtagacctcg
     1201 gcgaccaggc gggagaggat tgcggcttga tagccggagc cggtgccgac ctcgagcacg
     1261 cggtccgtgc gctggggatc gagttgctcg gtcatgaacg cgacgatgta cggttgtgag
     1321 atggtctgac catgcccgat gggcagcgga tagtcctcgt acgcgctctg ggcgacatcc
     1381 tgaggcacaa acgcgtgccg aggcaccgtc cgcatcgcct caagaacgcc cggattccgg
     1441 acatcgcggc cgcgccctgc gatttgttcc tgcaccattc gatgtcgttc ggagacacgg
     1501 ttcgagtcag gggccgtctg gtgatccata gcgctcgggt ggttgggccg cgggccgcac
     1561 ccggcgagcg ccgccagcag caggacggcg agcgcgtgca atccaggtgg aatcccgaga
     1621 gccatgatct caaggccggg ccatcgtcag cagccagcgc gaaacctccg agcaagcctc
     1681 cgcatccgcc ccgctgtaca aggctatggc ccgcgcttca ccgtccgcgt catatgcggc
     1741 cttcaccagg gcgggcgggc gatcaccctc gccggccagc cagagcctgc gcggcttgcc
     1801 cagcgcgatc atcccgggga gatccccgta aagcgcgccg cccggaagga agtcggggct
     1861 gtgcaaatcg aggaccttgc cgaaccgcaa acctcccgtg tcgagagcga ctgcatcgag
     1921 tgcgccccgc gcctgggcgc aggcgccggc aacccacggt ccgcctcctt cgagccccgc
     1981 caaaacgagt cgcccggtgc cgccccactg ttcttttgcg taggcaatgg ctgttagaac
     2041 atcatggacg cgttgcgcaa acagactgtg attgtatccg aacgtgtagg cggctgcctc
     2101 gcgcgggttc ttgacccgcc gggtctgggt gatgggcttg gcatcagcca ggaactctcc
     2161 ttggaagagg acatccaggc cgagtaccgt ccacccctgt tcgagcagag gccggagttc
     2221 ggatcggacc tcgcccgcgc tggtgtagag cccgctcttg ccggagccgc tgacccaaat
     2281 gacaaccccg gctttgtgtt gccgcggttc gagtcggatc gcgggcaact cctcgccatg
     2341 agtgaggttg cgcagtcggc cggttgtctg gtgacaggct tcgaggtcga cctgggcact
     2401 cgattcccag acgacctcgc cgacatcctt caaaccccgt ccgatcacgg catcgatgcc
     2461 tcctccgtag acgcgctcga actggtcgcg cgacgcggcc gcttcggcca attgcctggc
     2521 ggcatcttcg gttaaccggc gcagcaactt gcgctcgaac tccggtccgc cctcgggctt
     2581 gggatgctgg tcgttccaga ccgtgagctc ggctgtcgag agtcttctga agtcgcgctc
     2641 gatgatcggc tccttttgac cgaggccgag gtggcggttc atccagccgt acatcgccat
     2701 tcggctgacg ctgttgtaat tgtggccgaa gtgcagagcc tccttgagca tgacgttgtc
     2761 gggtgccccc agcagcgcgt agtggcgctt gagttccggg aagcctttgg tgcccatctc
     2821 cttggtccag tcgtccgcgg cggtcattcc ctgcggcttc ggagcaaaga gggcggcgaa
     2881 ctcgacgttg ccggtcccga tccgcaggcc gcaggcgttc tcacaggtgc agcccccctg
     2941 catggccgtc gagaccataa cggcgggaaa cgcgaccgcg ggccgcggat cgatcgcgca
     3001 caggatgaac gtctgcgttc cccctccgct cgcgccggtg acggctatcc ggtccgcgtc
     3061 cacgtcgggc aagccggtca ggaaatcgag ggcgcggatc gagttccatg tttgcaggcc
     3121 catgacactt tggaagtgcg cctcggcctg ggggctgaac aggccccagt tctcggttgt
     3181 gttcatctcg ggcctctgct ttgcgaaccc gtgcgccagc gcctgcgtga tctgcacgct
     3241 gtcggcgtac ccgatcatgt cgtagtggaa gaccacgcag cccattcgcg ccagcccgac
     3301 acagcgcgcc tgaagcgggc tgcgccccgc ttcctcgaat cgctccgctc ccgccgctat
     3361 ttcgcgccgc acatcctcgg ctccggtgtc caagaaacgt ccgttcgccc aatgcccatg
     3421 cgggcagagc actgcggggc gtttggcctg acccgggctt ttgggtcgat agagactgcc
     3481 ggtgacatag aaaccaggga ggctttcgaa gtagacgcgc tcgaccgtat aatcgtcgcg
     3541 ctcgatccgt ccgtggacga ccgcattcag gggcgttcgg gttggcgcgg gccacagacc
     3601 gagcgtcacg agcaattggc gccggaccct ctcggcgcgc ccatcccagg cggcgcggtt
     3661 cgacggggtg acaaagggga agtaaccgtc caaatccttg agtggttcga ggcggatgtc
     3721 gcgaggaagc tgccccggcg gcagggcgcg gggagcggcg gaatccgcgg cgaaagcgaa
     3781 cgcgaagcag gcagcgcagc cggaaagcaa ccgggcgcac gaaaggagct tggtggccat
     3841 gcgggtgagt gtaatgccgg gtccggccct cgccaagccc ggagagaatt ggggattggc
     3901 gatgagggta ctttctggtg atgtctgcgc cgatgattct gcacaagctc atcgtgcatc
     3961 acgccagaca cggatacgac catgcgttct cgctgatgca ggtggacgac gcggtccggt
     4021 ggctcaggga ccggggcgtt gcgatgggcc agggcacgag ggcgcttgat ctggggtgcg
     4081 gctccggcac gttcggtcag cgactgctcg aaatgggatg cagggtcacc tttgccgatg
     4141 aaagcgattg gctgcctgcg gagattccgc ggacgtgctt tcggaggatc aatctcgata
     4201 cggagtcgct ggagtcgctg ggccgccacg atctggtgat ttgctcgaat gtcctcgagc
     4261 atctgcgcga gtccgagcgg ctgatccggt cctttccagc cctgctggcg cccggaggac
     4321 atgtctattt gagctggacc aactggcttt caccctgggg gggccatgat ttttcgccat
     4381 ggcactacct ggggccgcgg cttggtcctc ggctcttcga ccgactggtc ggcaagccgc
     4441 gcgcgctcaa gccgttcgag aatctcttcg tgacccacat cggacgcact ctgcgacttc
     4501 tgcgggccca gcggcatctc gagctggtgg ccgtggcccc ccgctactac acggagctat
     4561 ggtctctgat gaagattccg gtgttgcgcg aggtgttggc gtggaactgc gcggcgcttg
     4621 ccgcgcgcag ggattagcgg gttcaaggaa gaaacaggaa gtacaaccgt ccggtctgaa
     4681 tgggcgggaa ctcgatacca agatcgacgt ggatggcggg cgtctctgtg cggacaaaca
     4741 gccgaaacaa attgcccagt tcgaaaggct gctcgtagcg gatgatcctg gcgcggtcga
     4801 tctgcgcgag cgttttggcc cggtccaggg ccgtcttgaa gttgccgagt tcatctacga
     4861 acccccaatc ataggcctgc cggccggaga atacccgccc gtccacatag tcctcccaat
     4921 tgtcgaccag cgcgcggccg ccgtcctcgc taaggcgata cgggctctgg cgtccttcga
     4981 ccaccacctc tttgaatctg cgaaatgtcg tgtccaccag agactgaacc atctcgcgct
     5041 cctccggcag gatatcctcc tcagccttgt cgccgcggag catgtccttg aagcgcccgc
     5101 tcttgtagac ctcaggccgc acgccgactt tgttcatcag gccgcggtaa ttgtaaccgc
     5161 gcataatgac gccaatgctg ccggtaatcg tgagggggtt ggccacaatc cagcggcaag
     5221 gcgcggcgac gtagtagccc cccgaggccg ccacgctccc catcgaggca acgatcggtt
     5281 tgccagagcg ctgctggaac tcggcaagac tccggttgat ctcgtccgac gcgagcacct
     5341 cgcctccggg cgagtcgatg cggagcagga ccgccttgac ggcatggtcg gattcggccc
     5401 gcttgagttg ctcgcgcacc atctcgacgg gtcccgacga ccctccaccg agcagacctc
     5461 cgaagataat cccctcgatg tcgatcaccg ccaccttgtc ccgccctcct tgatcgtcca
     5521 tcacaacttc atcgagccgg atcgatgtgc cgcgcgtcga cccgccagat aattggagca
     5581 gtccgattgc ccacctcccg acgctcgcca cggcgatgga gaggagaaca agcagaagca
     5641 ccaacgacag caccttccaa ccccagccgc tcgccggggg ccgcggggga gccgctgtgg
     5701 gtgggaccgc gcccaggacc ggaggggccg cttgcccttg cggacggtca tgcgaagccg
     5761 gcgcggcgag aggaggagca gccggggaat tcccggtcag gccggcggcg gactgcccat
     5821 ctggagacgt ctgaggcctc tggtcgtgtg caggattcgt tgtgttatcc ataggatggc
     5881 cggcacattg ggacgaaccc gccgccaatg caagccgcgg gcttgctggt gtcggggtcg
     5941 cgggcacaag ctcaccacgg caatgccgtt cagtcggtcg cccggcccgg aaatcctgtg
     6001 ctggtatgag gacactggtt tccgggctag gcggctggat cggggctggg cacggtcaat
     6061 cccagctcgg gtgcgatccc ttgcagtgcc tggatgacat ggttgatgtg gtcgtcgagg
     6121 ggaatctcga gcaaggcggc gccgcgttcg aggtcctcgc gcttgaccgc cgccgcgaag
     6181 gacttctgct tcatcttctt tcgcacactg ctcggggcca tcccggccag gcgctccgga
     6241 cgcacatagg ccacggccat cacgaagccg cacatttcat ccacggcgaa cagagtcttg
     6301 cgaaggggcc ggtccaacgg gtagtcgccc tgattccact ccgcgtggga gagcattgcg
     6361 cccacgatct cttcgccgac gccgcgttcc cgcaggaggc gggcgccttc gcgcgtgtgg
     6421 gccggcggtt cgggccaccg ttcgtagtcg aagtcgtgca caagacccac cagaccccag
     6481 agcgggacgt cctcgttgaa gtgcgccgcg tagtgccgca ttgcggcctc gacggccagg
     6541 gcgtgtttgc gcagagattc ggacgcggtg aactcggtca gcagccgc
//
LOCUS       DAOQRO010000180         6559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11661_length_6559_cov_5.200953, whole genome shotgun sequence.
ACCESSION   DAOQRO010000180 DAOQRO010000000
VERSION     DAOQRO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11661_length_6559_cov_5.200953"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /gene="secD"
                     /locus_tag="PKK20_07905"
     CDS             complement(<1..1368)
                     /gene="secD"
                     /locus_tag="PKK20_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNU99851.1"
                     /translation="MSRNVWKTSLALTILAIAWALYSIYPPTPRDLIRVFEKQAEYVD
                     TNFTAIVEQARSLHQANPDHAFRNLQTAVGTNDLIRYFPSLADEETADQNRHILNQIQ
                     RRAAGKIKLGLDLQGGTSFLVAMDTNRFENVVDKRHALEQAMEVLRKRVDRFGVAEPQ
                     LQPQGENRILIQIPGLSEADKESAKSTLQRAAFLEFRMVHPQSAELVQQEIVEPGYEI
                     KEERIRRRAGQEPQPIRRYLVRKTPERGLTGKHVDRSGVYLDPVTGSPRINLLFNPEG
                     ASLFADITREHVGHQMAIVLDGELYSAPVIRTEILGGSCEISGDFDLREANELANVLQ
                     NPLEAPVHIEEERTVDPSLGQDSIDSGFRASIIAAAGTFVFMIIFYFASGLVANVALA
                     LIIFLLMGAMCAMDSTLTMPGIAGIALAVGMAVDANVLIYERMREEIAAGKSIMWILG
                     DLTTGA"
     gene            complement(1400..1744)
                     /gene="yajC"
                     /locus_tag="PKK20_07910"
     CDS             complement(1400..1744)
                     /gene="yajC"
                     /locus_tag="PKK20_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNU99852.1"
                     /translation="MIENMTGISWCLLAQQSAQDPKAGMIQMLGMIAIMGFMFYFALW
                     RPQQKKAKEHEALVKALKAGDKVVTSSGICGVIVSVKDKTASLRSADSKLEVLKSAIA
                     QVIERSTSNDQN"
     gene            complement(1839..3173)
                     /gene="xylA"
                     /locus_tag="PKK20_07915"
     CDS             complement(1839..3173)
                     /gene="xylA"
                     /locus_tag="PKK20_07915"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNU99853.1"
                     /translation="MKKRTARAEFFPGIAPIRYEGPDSKNPLAFKHYNPEEMVEGKPM
                     KDHLRFSVVFWHTLRGVGADMFGMNRSWHRPWEDGSDSLEMALKRVAVLFEFCRKLGA
                     PFYAFHDRDVAPEGRTLRETNRNLDRVVKELKKHQADTGVGLLWGTACLFAHPRYMHG
                     AATSCNADVFAHAAAQVKKAIEVTHELDGAGYVFWGGREGYSTLLNTDMKRELDHLGA
                     FLHMAVDWKKKIGFKGQFYIEPKPKEPTKHQYDSDAAACLNFLREYDLMGHLKLNIET
                     NHATLAGHTMQHDLEVAGAAGALGSIDANTGDLLLGWDTDQFPTDVYLTTQIMLTLLK
                     YGGLTTGGTNFDAKCRRESFEPVDLFHAHIGGMDAFARGLKIAAAIRKDGRLAEFVRH
                     RYSSWDGDIGRRIERGEVGFKELEAYVLKKGRLAPNASGRQEFLENLINEFV"
     gene            3278..3706
                     /locus_tag="PKK20_07920"
     CDS             3278..3706
                     /locus_tag="PKK20_07920"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNU99854.1"
                     /translation="MSKPIFPRSPRETMCGWVHLPRYVDKIRLHLAGRLHPDYHANLG
                     QGFDGRWLAAAGVAHAQMVEVVRQSITDGEVCDWVRCNVRKTDAEKAAHRQEVLGFPA
                     STDTAMQSLLERRKAEAGMADRAEITTFVDFIDADEGRFE"
     gene            3766..4194
                     /locus_tag="PKK20_07925"
     CDS             3766..4194
                     /locus_tag="PKK20_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99855.1"
                     /translation="MAENTPNTPEAPAPPKPAEAAKVQPKKETVRISLPPKPTAAPTI
                     KIPTPAPVATPTAAAAAAPVSGAAPAPAAPAVPVPKPVAVAPPSAAAAAAPRAPSRRA
                     AASSATGITMLDNGLAVAAAVLGLAALVSVFLLLRMLQFQ"
     gene            4258..4596
                     /gene="trxA"
                     /locus_tag="PKK20_07930"
     CDS             4258..4596
                     /gene="trxA"
                     /locus_tag="PKK20_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNU99856.1"
                     /translation="MGFEKILTLTAETFDGEIAGSPVPMLVNFWADWCAPCHSIASLL
                     DDLAIEYDGQVRFGRVNVDIEQRLAAEYRISGVPTLLLFKDGQVQDVIVGLKNRQDLV
                     ASLEQALTPA"
     gene            4621..5619
                     /locus_tag="PKK20_07935"
     CDS             4621..5619
                     /locus_tag="PKK20_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU99857.1"
                     /translation="MPLIVTPGRLARLGEFYYQLKSMQAAGLSLIQALDQLRLASPSR
                     SLVRPIERLLDGLNHGLSFTEALKSLGRWLPSFDLALLAAGEQSGRLDSVCGLLAQYY
                     QNRAALARQVISSLIYPAVIVHAAILLGPFPQFFLSGDLVTYARQTLGTLLPIYGVVL
                     LGVMACQSRRGLFWRSAVERLLQLIPVLGRARRSLALARLTVALDALLEAGVSAVEAW
                     PLAAAASGSPALGRDVAGWKTRLLGGDAPGDLLPRARSFPSFFSNLYRTGEISGTLDD
                     ALKRLRVYYEDDAQRKMKALAFWVPFAIYIVILILLAMTVVGFWVGHYNAILDTDY"
     gene            5642..>6559
                     /locus_tag="PKK20_07940"
     CDS             5642..>6559
                     /locus_tag="PKK20_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNU99858.1"
                     /translation="MTVDEILSNEPLRLEEFPVARRAVYLAHAGVCPLPRRVQAALTA
                     YAAQGTTGDQEEAVPPSFLAEARQAAARLIGAAPSEIAFVGPTSLALSFVAAGLPWRR
                     GDNVLLYADDYPSNVYPWMALASRGIEVRTHETDALGRIVPEQVLARADDRTRLVALA
                     SCHFVSGWRLEYQEIGRRLRERGIWFCLDGIQTLGAFPLDASCVDFLAADAHKWLLGP
                     CAAGILYVRKELQPQLAPLAFGWNNVRCPGFVAEPELVLQGDARRYEAGSANLLGLVG
                     LKAACDLILEFGVDAIAAELLRKRAWLIAA"
BASE COUNT         1048 a         2066 c         2159 g         1286 t
ORIGIN      
        1 ggcgccggtg gtgaggtcgc cgaggatcca catgatcgac ttgccggcgg cgatttcctc
       61 gcgcatgcgc tcgtagatca ggacgttggc gtcgacggcc atgccgacgg cgagggcgat
      121 gccggcgatg cccggcatcg tcagcgtgct gtccatggcg cacatggccc ccatcaggag
      181 aaagatgatc agtgccagcg ccacgttggc gaccaggccg gaagcgaagt agaagatgat
      241 catgaacacg aacgtgccgg ctgccgcgat gatcgaggcg cggaatccgc tgtcgatcga
      301 gtcctgtccc agagagggat cgaccgtgcg ctcctcctcg atatggaccg gcgcttcgag
      361 gggattctgg aggacattgg ccagttcatt ggcctcgcgc aggtcgaagt cgcccgagat
      421 ctcgcacgag ccgcctagaa tctcggtgcg gatgaccggc gcggagtaga gttcgccatc
      481 gaggacgatg gccatctgat ggccgacgtg ctcgcgggtg atgtcggcga acagggacgc
      541 tccctcgggg ttgaagagca ggttgattct tggggagccg gtgacaggat ccagatagac
      601 gcccgatcgg tccacatgct tgcccgtgag gccccgctcg ggcgtcttgc ggaccaggta
      661 cctgcggatc ggctgaggct cctggccggc gcgacggcgg atgcgctcct ctttgatctc
      721 gtaaccgggc tcgacgattt cctgttggac cagctcggcg ctttgcgggt ggaccatgcg
      781 gaattcgagg aacgcggccc gctgcagggt gctcttggcg ctctccttgt cggcctcgga
      841 caggccggga atctgaatca gaatccggtt ctcgccctgg ggttgaagtt gcggttcggc
      901 cacgccgaag cggtcgacac gcttgcgcag gacttccatc gcctgttcga gggcgtggcg
      961 tttgtcgacc acgttctcga agcggttggt gtccattgcc accaggaacg atgtgccgcc
     1021 ctgcaggtcg aggcctagct tgatcttgcc ggcggccctg cgctgaatct ggttcaagat
     1081 atggcgattc tgatccgccg tttcttcgtc ggccagggac gggaaatagc ggatcaggtc
     1141 gttcgtgccc accgccgtct ggaggttgcg gaaggcgtgg tccgggttgg cctgatgcag
     1201 gctgcgcgcc tgctcgacga tggccgtgaa gttggtgtcc acgtactccg cttgcttctc
     1261 gaagacccgg atcaagtcgc gcggggtggg tgggtaaatg gagtataagg cccaggcgat
     1321 cgcgaggatc gtgagagcca gggaggtttt ccagacgttg cgactcatgg cgggggggga
     1381 gactctcggg aacgaaaggc tagttttgat cgttgcttgt gctgcgctcg atcacctgtg
     1441 cgatggcgct cttgagcact tccagcttgg aatcggccga ccgcagggag gcggtcttgt
     1501 ctttgacgct gacgatgacg ccgcagatgc cgctgctggt gaccaccttg tcaccggcct
     1561 tcagggcctt gaccagcgcc tcgtgttcct tggccttctt ctgctgcggc cgccaaaggg
     1621 cgaaatagaa catgaaaccc atgatggcga tcatgcccag catctggatc atgcccgcct
     1681 tcgggtcttg cgccgattgt tgcgccagca aacaccaaga aatacctgtc atgttttcga
     1741 tcaaagatgg gcgagaatct agggagattc cccgtttggc aagcacggat gcgctcaaaa
     1801 aatcggagcg cgcctcgccg ggcgatcatg ccggcgtctc aaacgaactc gttgatcagg
     1861 ttttcgagga actcctggcg tccgctcgcg ttgggggcca ggcggccctt cttgagcacg
     1921 taggcctcca gttccttgaa cccgacctcg ccccgctcga tccgacgccc gatatcgccg
     1981 tcccatgaac tgtagcggtg ccgaacgaat tcggccagac gaccatcctt gcggatggcc
     2041 gccgcgatct tcaggccgcg cgcgaaggcg tccatccctc cgatgtgcgc gtggaacagg
     2101 tccaccggct cgaagctctc gcggcggcat ttggcgtcga agttggttcc gcccgttgtc
     2161 aggccgccat acttgagaag ggtgagcatg atctgcgtgg tcagatagac gtccgtgggg
     2221 aactgatcgg tatcccagcc cagcagcaag tcgccagtgt tggcgtcaat gcttccgagc
     2281 gctccggccg cccccgcgac ctcgaggtcg tgctgcatcg tgtggcccgc gagggtggca
     2341 tggttggtct cgatgttcag tttgagatgg cccatcagat cgtactcgcg caggaagttc
     2401 aggcaggccg cggcgtcgga gtcgtactgg tgtttggtcg gttccttcgg tttgggctcg
     2461 atgtagaact ggcccttgaa cccgatcttc tttttccagt cgaccgccat gtgcaggaag
     2521 gcgcccaggt gatcgagctc gcgcttcatg tcggtgttca ggagcgtcga gtaaccctcg
     2581 cgccctcccc agaaaacgta gcccgcgccg tcgagttcgt gcgtgacctc gatggccttc
     2641 ttgacctggg ccgcggcgtg ggcaaaaacg tcggcgttgc aactggtggc cgccccatgc
     2701 atgtagcgcg ggtgggcgaa caggcaggcg gtgccccaca ataaaccgac gccggtgtcg
     2761 gcttggtgct tcttcaactc cttgacgact cgatccaagt tccggttcgt ctcgcgcagg
     2821 gtccgcccct ccggcgcgac atcccgatcg tggaacgcgt agaacggagc gcccagcttc
     2881 cggcagaact cgaagagcac agccacgcgt ttgagcgcca tctcgagcga atcggacccg
     2941 tcttcccagg gacgatgcca cgagcggttc atgccgaaca tgtcggctcc gaccccgcgc
     3001 agcgtgtgcc agaagacgac cgagaatcgc agatgatcct tcatcggctt gccctcgacc
     3061 atctcctccg gattgtaatg tttgaaggcc aaggggtttt tcgagtcggg cccttcgtag
     3121 cggatggggg cgatgccggg gaagaactcc gcgcgtgcgg ttcgtttttt catgcgtgtt
     3181 gtcagctttt gggcgcacat tagcggggcc gggcggacgt gacaagacgt gtcttcggcg
     3241 cattgacacg agcagggcat cctccatact ggcgggcatg agcaagccaa tcttcccacg
     3301 cagtccgcgc gaaacaatgt gcggctgggt ccatctgccc cggtacgtcg acaagattcg
     3361 cctccacctg gcgggccgac ttcaccccga ctaccacgcg aacctgggcc agggctttga
     3421 cgggcgctgg ctggcggcgg ccggcgtagc gcacgcccag atggtcgagg tggtgcgcca
     3481 gtcgatcacc gacggcgagg tctgtgactg ggtgcgctgc aacgtgagaa agaccgatgc
     3541 cgagaaggcg gcgcatcgcc aggaggttct cggttttccg gcatcgacgg acaccgcgat
     3601 gcagagcctg ctcgaaaggc gcaaggcgga ggccggcatg gcggatcgcg ccgagatcac
     3661 gacttttgtg gacttcatcg atgccgacga ggggcggttc gagtagtgtg gccgaatagt
     3721 gtttgacact ctggaagtcg attcctagtt tgacgaggca attttatggc agaaaacaca
     3781 ccgaatacgc ccgaggcgcc ggctcctccg aagcccgccg aagctgctaa ggtgcagccg
     3841 aagaaagaga ccgtgcggat cagtctgcct ccgaaaccga cggcggcgcc cacgatcaag
     3901 attcccacgc cggctccggt ggcgactccc accgccgccg cggcggcggc gcccgtgtcg
     3961 ggcgctgctc cggcgcccgc tgcgcccgct gttccggtgc caaagcctgt cgccgttgct
     4021 ccgccctcgg ccgctgccgc ggctgccccg cgcgcgccat cgcgacgggc tgcggcatct
     4081 tccgcgaccg gcatcacgat gttggacaac gggctggcgg tggcggcggc agttctgggc
     4141 ctggcggcat tggtgagcgt ctttctgttg ctgcgcatgc tccagttcca atgaggcgtc
     4201 ggcgaccggt ctgacaaccg gccggccgtt ctccgaattc cgaaaggggc tgcacacatg
     4261 ggcttcgaga aaatccttac gctcaccgcg gagacgtttg acggtgagat tgccgggtcg
     4321 cctgtcccca tgcttgtgaa tttctgggcc gattggtgcg ccccgtgcca cagcatcgcg
     4381 tccctgctcg acgatttggc catcgagtac gatggccagg tccgcttcgg cagggtcaac
     4441 gtcgacatcg agcagcggtt ggccgccgaa taccgcatca gcggcgtgcc gacgctcctt
     4501 cttttcaagg acggtcaggt gcaggacgtc atcgtggggc tgaagaaccg gcaggatctc
     4561 gtcgccagcc tcgaacaggc gctcactccc gcatgagccc ctggcgcaag ccgtgccgtc
     4621 atgcctctga ttgtcacgcc gggtcggctc gcgcggctgg gggaattcta ctaccagctc
     4681 aagtcgatgc aggcggccgg cttgagcctc atccaggcgc tcgatcagct tcggctcgcc
     4741 tcgcccagcc gatcgctggt ccgcccaatc gagcgtctgc tcgacggatt gaaccacggg
     4801 ctctctttca ccgaggcgct caagagcctc ggacgctggc tgccgtcatt tgacctcgca
     4861 ctgctcgccg cgggcgaaca gagcggacgc ctcgattcgg tctgcgggct cctggcccag
     4921 tactaccaga accgcgccgc actggcgcgc caggtgatct cctccctcat ctatccggcg
     4981 gtgatcgtcc atgccgccat cttgctcggt ccattcccgc agttctttct gagcggcgat
     5041 cttgtcacct atgcccggca gacgttgggc accctcctgc cgatctacgg cgtggtcctg
     5101 cttggagtca tggcctgcca gagccggcgt ggactattct ggcgctccgc tgtcgagcgc
     5161 ctgcttcagc tcatccccgt gctcggccgg gcgcggcgaa gcctggccct ggcgcgcctc
     5221 acggtggcgc tcgatgccct gctcgaagcc ggggtgtccg cggtcgaagc ctggcctctg
     5281 gcggccgcgg ccagcgggtc gccggcgctc gggcgcgatg tggcgggttg gaagacgcgc
     5341 ctgctgggcg gcgacgcgcc gggcgacctg ctgccgcggg cgcgctcttt cccgagtttc
     5401 ttctcgaatc tttaccggac gggggagatc agcggcacgc tcgatgacgc cctcaaacgc
     5461 ctgcgcgtct actacgagga cgacgcccag cgcaagatga aggctctggc cttctgggtc
     5521 cctttcgcca tctacatcgt gattctgatc ctgctcgcca tgacagtcgt cgggttttgg
     5581 gtcggccact acaacgcaat cctcgatacg gactactagc cgtcggacgc atccgctcct
     5641 tatgaccgtt gacgaaatcc tctccaacga accgctgcgc ctcgaagagt ttcccgtcgc
     5701 gcggcgcgcc gtttacctgg cccatgccgg cgtttgcccc ctgccgcgcc gcgtacaggc
     5761 cgccttgacc gcctatgccg cgcaaggcac cacgggcgac caggaggagg cggtgccgcc
     5821 ctcgtttctg gcggaagcgc gtcaggccgc cgcccggttg attggcgccg cgccatcgga
     5881 gattgccttt gtggggccga cgtctctggc gttgagcttt gtggcggcgg gtctgccgtg
     5941 gcgacgaggc gacaatgtgc tgctgtatgc ggacgattac ccgagcaatg tctacccgtg
     6001 gatggccctg gcctcgagag ggatcgaggt gcgcacgcac gagacggacg cgctggggcg
     6061 gatcgtgccc gaacaggttc tggcacgcgc ggacgatcgc acgcgcctgg tcgcgcttgc
     6121 ctcctgccat ttcgtctccg gctggcgcct cgaataccag gagatcgggc ggcgcttgcg
     6181 cgaacgggga atctggtttt gtctcgatgg aatccaaacg ctcggcgcct ttccgctgga
     6241 cgcgtcgtgc gtggatttcc tggccgcgga cgcccacaag tggttgctgg ggccgtgcgc
     6301 cgccgggatt ctctacgtgc gcaaggaact gcagccccag ctcgccccgc tggcctttgg
     6361 ctggaacaac gtccgctgtc cgggctttgt ggcggaaccc gagctggtct tgcagggcga
     6421 cgcgcgccgt tacgaggccg ggagcgcgaa cctccttggc ctggtcgggc tcaaagcggc
     6481 ctgcgatctg attctcgaat tcggagtgga tgcgattgcc gccgaactgc tgcgcaagcg
     6541 ggcttggctc attgccgct
//
LOCUS       DAOQRO010000181         6552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11682_length_6552_cov_6.588425, whole genome shotgun sequence.
ACCESSION   DAOQRO010000181 DAOQRO010000000
VERSION     DAOQRO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11682_length_6552_cov_6.588425"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            472..3084
                     /locus_tag="PKK20_07945"
     CDS             472..3084
                     /locus_tag="PKK20_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HNU99859.1"
                     /translation="MRTRFRIAWASLTTLIVAGCHTTPPEQVGPIKEGGHLAPTHQLL
                     RPAGESVEFGGRPVDLILAPNRRTVWVKDNRGLVAIDTADWSVRQELKFKESGGSTHG
                     LAVTRDGTRLFASGAANALWEAQIDAQGVATWGRKITLPGPGGKGNPHVGGIALSRDE
                     TKAYVCLSRNNTLAVVDLSAGTIVQEIAVGVAPYDVACANGDGNESVAFVSNWGGRHP
                     VGNEPAANSSDTPALVDERGVASSGTVSVVDLAQGREVAQIATGLHPSDLEFGRGGDL
                     LYVANANSDSVSVIDVARRVMVGEALVRPASGLPFGSAPNALALSPDGRRLYVANGGN
                     NAVAVLNLDQPIEPRVEGFIPAGWYPSALFADRSHLYVANAKGIGSRHPDPKKKGWNS
                     HQHRGSVTRVAIPTPGQLADYTRQVRADARVPEILRAWERSNQARRPVPVPKRLGDPS
                     VFEHVVYIIKENRTYDQVFGTLTQANGDASLCVFGREVTPNHHALAEQFVLLDNFYCN
                     GVLSADGHAWATEGYVTDYLEKSFGGFTRSYPFAGDDPLSYAPTGFLWDNVLLHGLSF
                     RNYGEMNITATQPANATFKQIYEDYLAKTGKIGFKHNIEIDTLRRYSCPASPGWNMRI
                     PDQIRADAFLDEFRAFERDGHWPNLVILYLPNDHTSGTSAGSPTPQAQVADNDLALGR
                     VVDAISHSRFWPKTCIFVIEDDPQNGFDHVDGHRSLCLVVSPYTKRGAVISQFYNQTS
                     VLHTMQRMLGIPPMNQMDALSPLMTACFQSKADLTPYVCLPTNIPLDEMNKETSDLSP
                     QERHWAESSAVLPLDQPDQADEDTLNRIVWHSMRGADEPYPAAFAGAHGRGLKRLGLR
                     LDNLVRAPAPGRPE"
     gene            complement(3200..3373)
                     /locus_tag="PKK20_07950"
     CDS             complement(3200..3373)
                     /locus_tag="PKK20_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99860.1"
                     /translation="MDSRTMEGFRASGVTRAREPDRGGSCRRLLGVAYAPYLGYSLMP
                     WFEALDQPGATQM"
     gene            complement(3454..4836)
                     /locus_tag="PKK20_07955"
     CDS             complement(3454..4836)
                     /locus_tag="PKK20_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU99861.1"
                     /translation="MFPDTIVSTVTVRGETSPFSARLRGLLSLLVMLGAAGAGGHAAN
                     WPAWRGPDGNGICRETRLPLRWSTNENVRWQTPLPMLGNSTPVVWGNRVFLTQTVAEE
                     SRRSLYCLDLRKGDILWQAGPVWTGPDSGGDQNPPCTPSAVTDGRRVIAWFGSAGVFC
                     YDLKGRELWRRDLGRQSHAWGYASSPVIYRGLCLLNFGPGERAFVIALDLRNGRRVWQ
                     HDVPPIPADAKWEAYGGNVNDWKRLGSPAVAEVTGSCATPLLVSASGRAEAVVPLPMR
                     LVAFAPSTGAVLWTCEGPNTGAYSSPFDGDGLVAAFGSGLRNTALVVRPGGSGDVTAT
                     HRVWNRLPANSKACLGSGVIAGGRIYQVTMMGFAQCIDLRTGETVWEERLTGTGARNG
                     FWSSPILAGDRIYAANQNADVFVLRAAPRFECLATNSLGGELMNASPAAARGAILLRT
                     ERRLWCIAER"
     gene            complement(5198..>6552)
                     /locus_tag="PKK20_07960"
     CDS             complement(5198..>6552)
                     /locus_tag="PKK20_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99862.1"
                     /translation="FSPDGKVLGPLDQFSLTRDPDSRPPLTPVITGPSSFDAFEGGLL
                     ELACSAFADPDSSSVHMQSRWRLSFSPDVEAVGGVLFERVTGPGVTNCAIAATNIWPG
                     QVVYASVQHTAGDGLVSAFASPIAIRITPDPLFLEDFESVAEFSLPAGWTATHRTSVL
                     SNIWDPDDPRSNTYLTWTVVSSSRLHALGANRLSVPGIVRGNSVYAESDHRSGVQIQY
                     LTTPDYDLRGFENIRLVFRSNYMQNQDSLAALEFSIDRGATWLPVVYLLDSKDVVFRP
                     GGVTIDAAATFGRVDPDGVPNASGTAASGGTYGEHILSRPFEDLAPHIQGRINDDPIE
                     SKRIECFRLPAADRQAAVRFRFVLVGTASWFWGIDDFGLFGSADALDPLRIVSATARQ
                     DELHLAWTGPDGPYRVQVRDALDAGSWVDYGAAIDADRRSVVLPIGGITGFYRVRLDR
                     "
BASE COUNT         1317 a         2083 c         2040 g         1112 t
ORIGIN      
        1 cacagattcg gggaggaaca cagattgccc ggtcatggcc ttctccgtgt cctcggcgtc
       61 tctgtggttc attccgtacg gtgacagtca gcgaggggga tcaaccacag aggcacagag
      121 agcgcggaga tcggagatgg gagatgggag atcgggtatc gactgccgga gctgattttc
      181 tcgctgacgc acctccgttg aagcggctcg tcttgcggcg gaggtcttca gccgcaaaat
      241 aaagtgacgg tgcggaccgg acagagggaa gactctgaga ggttgcactt cgatccgacg
      301 ctagtccagg ccgcgctctc tgtctctctg gttccgcctt ccaaataccg tcgagcaaaa
      361 attcaaacaa acgcgcaaat ccgcaaatcg gtgcactggc ggcagattgt cgagatcccg
      421 agttgctccg cctcacggca tccactacac tcggcgcaca aatcctccgc tatgagaacg
      481 cgttttcgca tcgcatgggc gtcgctgacg acgctgatcg tcgccggatg ccataccacg
      541 cctcccgaac aggtcggtcc gatcaaagag ggaggtcatc tggcccccac ccatcaactc
      601 ctgcgccccg cgggcgaatc ggtcgagttc ggcggtcgtc ccgttgatct catcctggct
      661 ccgaatcgcc ggacagtctg ggtcaaagac aaccgcggac tcgttgccat cgacacggcg
      721 gactggtccg tgcgtcagga gctgaagttc aaggagagcg ggggctcgac gcatggcctg
      781 gccgtcacgc gcgacgggac tcggctcttc gcgtcgggcg cagccaacgc cctgtgggaa
      841 gcgcagatcg acgcgcaagg cgtcgcaacc tggggcagga agatcaccct gcccggcccg
      901 ggtgggaaag ggaaccctca cgtgggggga atcgccctgt cgcgggacga gacgaaagcc
      961 tatgtctgtt tgtcgcgcaa caacacgctg gcggtggtgg atctcagtgc gggaacaata
     1021 gtgcaggaga tcgcggtcgg tgttgcgcct tacgatgtgg cctgtgcgaa cggagacgga
     1081 aacgaatcgg tggcgttcgt ttcgaattgg ggagggcggc atccggtggg caatgagccc
     1141 gcggcgaact cctcggacac gcctgcactg gtcgacgagc gcggcgtcgc gtcgagcggg
     1201 acggtctcgg tggtggattt ggcccaggga cgcgaggtcg cccagatcgc aacgggcctg
     1261 catccgtccg acctcgagtt tggcaggggg ggtgacctgc tctacgtggc caacgccaac
     1321 tcggactcgg tgagtgtgat cgacgtcgcg cggcgggtga tggtcgggga agcgctcgtc
     1381 cgaccggcat ccggtttgcc ctttggcagc gctcccaatg ccctggctct gtcgccggat
     1441 ggccgccggc tttacgtggc caacggcggc aacaacgccg tcgccgtgct caacctggac
     1501 caaccgatcg aaccgcgcgt cgagggattc atccccgcag ggtggtatcc gagtgcgctc
     1561 ttcgccgaca gatcgcatct ctacgtggcc aatgccaaag ggatcgggtc gcgccatccg
     1621 gacccgaaga agaagggctg gaacagccac caacatcgcg gcagcgtgac gcgcgtggcg
     1681 attccgacac cgggtcagct cgcggactat acccgacagg tcagggcgga tgcccgggtg
     1741 ccggagattc tgagggcctg ggagaggtcg aaccaggcgc ggcgtccggt gccggtgcca
     1801 aagcgccttg gggacccgtc agtcttcgag catgtggtgt acatcatcaa ggagaaccgc
     1861 acctacgacc aggtattcgg cactctcact caagccaacg gcgacgcgtc gctgtgcgtt
     1921 ttcggacgcg aggtgacgcc caaccaccat gctctggcgg agcaattcgt gctcttggac
     1981 aacttctact gcaacggggt gttgtcagcc gacggtcacg catgggccac ggagggctac
     2041 gtcaccgatt acctcgagaa atcgttcggc ggattcaccc gcagttatcc cttcgcggga
     2101 gacgatccgt tgagctatgc gcccacgggc tttctctggg acaacgtgct cttgcacggg
     2161 ctctccttcc gcaattatgg agagatgaac atcacagcca cacagcctgc caacgccacg
     2221 ttcaagcaaa tctacgagga ttacctcgcc aagaccggca agatcggttt caagcacaac
     2281 atcgagatcg acaccttgag gcgctacagt tgcccggcat cgccgggctg gaacatgcgc
     2341 atcccggatc aaatccgggc cgatgccttt ctcgatgaat ttcgggcgtt cgaacgtgac
     2401 ggacactggc ccaacctggt cattctctac ctgcccaacg accacacctc gggcacaagc
     2461 gccggcagtc cgaccccgca ggcgcaggtc gccgacaatg acctggcgct gggtcgagtc
     2521 gtggacgcca tttcgcacag ccgcttctgg cccaagacct gcatcttcgt gatcgaagat
     2581 gatccccaga acggattcga ccatgtcgac ggccaccgct cgctctgtct ggtggtcagt
     2641 ccgtacacga aacggggcgc cgtgatcagc cagttctaca accagacctc cgtgctccac
     2701 accatgcaac ggatgctcgg catcccgccc atgaaccaga tggacgcctt gtcccccctc
     2761 atgaccgcct gctttcaaag caaagccgat ctgacaccgt acgtctgtct gcccacaaac
     2821 atccccttgg acgaaatgaa caaggagacc agcgacctct cgccacagga aaggcactgg
     2881 gccgaatcga gcgcggttct cccgctggac cagcccgacc aggcggacga ggacacgctg
     2941 aaccggattg tctggcactc gatgcgaggc gccgatgaac cctatcccgc ggcctttgct
     3001 ggggctcacg gcagaggcct gaaacgcttg ggcctgcgcc tcgataacct ggtccgagca
     3061 cccgcgccgg gcaggccgga gtgacgcctc ggaacactgt agtccctcgc agcgtcgtcc
     3121 agcacgagca cccaatcaag gtgctcgccc aaggcacgcg aatgaggaac ggacgcgaga
     3181 gcatcaggtt cttggcgtgc tacatctgcg tcgcgccggg ctgatcgagc gcctcgaacc
     3241 agggcatcag gctgtagccg agataaggcg cgtaggccac ccccagtagg cgtcggcatg
     3301 atccgccgcg atcaggttct ctcgctcgag tgacaccgga cgcccgaaat ccctccattg
     3361 tgcgcgaatc caccgcattc agttccaccg aggatgagcg cgactctgcc ggtcatcctg
     3421 ggagagcacg taagacgcct ggatttctcc ctctcatcgt tcggcgatac accacaaacg
     3481 cctctccgtt cgcaacagga tggcgccgcg cgcggccgcg ggcgaggcgt tcatgagttc
     3541 tccgccaagg gagttcgtgg cgaggcattc gaaccgcgga gccgcccgaa gcacgaagac
     3601 gtccgcgttc tgatttgccg cgtagatgcg atccccagcc aggattgggg aagaccagaa
     3661 gccgttgcgc gcgccggtgc cggtgagacg ttcttcccag acggtttcgc ccgtgcggag
     3721 gtcgatgcac tgagcgaagc ccatcatggt gacctggtag atgcgcccgc cggcgatgac
     3781 cccggaaccg agacaggcct tgctgtttgc cggcaaccga ttccacactc gatgcgtggc
     3841 tgtgacgtcg ccgctcccgc cgggccgcac cacgagggcg gtattgcgga ggccgctccc
     3901 gaaggccgcc acaagcccat cgccatcaaa cggcgagctg taggcgccgg tattcggtcc
     3961 ctcacaagtc cacaggaccg cgcccgttga aggcgcgaag gccacaagtc gcatcgggag
     4021 tggcaccaca gcttccgcgc gaccggacgc cgagaccagc aagggcgtcg cacaggagcc
     4081 ggtgacctcg gcaacggccg gcgatcccag ccgcttccaa tcgttgacgt tgcctccata
     4141 ggcctcccat ttcgcatcgg ccgggatggg cggcacatcg tgttgccata ctctgcggcc
     4201 atttcggagg tccagggcga tgacgaaagc ccgctcgcct ggcccgaaat tgagcaggca
     4261 aagtccacga taaatcaccg gcgacgacgc gtaaccccaa gcatgggatt gtcggcccag
     4321 gtcgcggcgc cacagctcac gacctttcag atcgtaacag aagacgccgg cggatccgaa
     4381 ccatgcgatc acccgccgcc catcggtcac cgccgaaggt gtgcatggag gattctggtc
     4441 gccgcccgaa tcgggtcccg tccaaaccgg ccccgcctgc cagagaatgt ctccctttct
     4501 gaggtccaga cagtagaggc tgcgacggct ttcctcggcc acggtttggg tgaggaacac
     4561 gcggttgccc cagaccaccg gtgtcgaatt gccgagcatc ggcaacggcg tctgccagcg
     4621 cacattctcg ttggtcgacc agcgcagcgg aagccgtgtc tcccgacaaa tcccgttgcc
     4681 atcaggcccg cgccacgccg gccagttcgc cgcgtgcccg ccggcgccgg ccgctcctaa
     4741 catcacaagc aaagacagaa gtccacgaag tcgggcggag aacggcgagg tttcgccccg
     4801 cacggttaca gtcgagacaa tggtgtcagg gaacatccga cgcagtctgc ccaccaaaac
     4861 gcggcggtga caagtggaat ggccggaatc cttgaccgct gaaacatgtt gcggacaagt
     4921 cgagtttccg cgctgcgttg aaggggtcaa agattccaat gaagctccgc ctttgcctca
     4981 acggctacat cttgacccga tttcatggaa agttttgaca ttcagcggtc gaatgcgcac
     5041 ggggaccatg aaccgatttg tagccgccga cgtgagtcgg cgctgatttc tgcttttgac
     5101 ggagcgagat ggcgcggact cacgtccgcg gctacggaac cacggttcat ggaacgagga
     5161 ggaggactgg atttggtctg atgcgcgcct cgttagatca ccgatccaag cggacccgat
     5221 aaaagccggt gatgccgccg atgggcagga ctacggaccg tcgatcggcg tcgatcgcgg
     5281 cgccgtagtc gacccatgag cccgcatcga gcgcgtcccg gacttggacc cggtaagggc
     5341 cgtccggacc ggtccaagcg aggtggagtt catcctgccg agccgtggcg ctgacaatcc
     5401 ggagcgggtc cagagcgtca gcggacccga agagaccgaa gtcatcgatt ccccaaaacc
     5461 aactggctgt ccccaccagt acgaaacgga accggacggc ggcctggcgg tcggcagcag
     5521 gcagccgaaa gcactcgata cgcttcgact cgatcgggtc atcgttgata cgaccctgga
     5581 tatgcggtgc gaggtcttca aacggacgac tcaagatgtg ttcgccgtag gttccgcccg
     5641 acgccgcggt accgctggcg ttgggcacgc cgtccgggtc tacacgaccg aacgtggccg
     5701 ccgcatcgat ggtcacgccg ccgggacgga aaacaacatc cttgctatcg agcaaataga
     5761 ccacaggaag ccacgtggcg ccccggtcga tcgagaactc gagggccgca agactgtctt
     5821 ggttctgcat gtagttgctg cgaaagacca ggcggatgtt ctcgaacccg cgcaggtcat
     5881 aatcgggagt cgtcaggtat tgaatctgga caccgctccg atggtccgac tcggcgtaca
     5941 ccgaattgcc gcggacaatg cccggcacgc taagccgatt cgcccccaga gcgtgcaaac
     6001 ggctgctcga gacgactgtc caggttagat aggtgttcga ccgcgggtcg tctggatccc
     6061 agatgttcga aagaacgctc gtgcgatgcg tggccgtcca tccggcaggc aggctgaatt
     6121 cggccacgct ctcgaaatct tcgaggaaaa gcggatcggg cgtgatccgg atcgcaatcg
     6181 gagacgcaaa tgcgctcacc aatccgtctc ccgccgtgtg ctgaacgctc gcgtaaacga
     6241 cttgccccgg ccagatgttg gtcgcggcga tcgcgcagtt ggtcacgccg gggccggtca
     6301 cccgctcgaa aaggactcca ccgaccgcct cgacatccgg actgaaactg agccgccacc
     6361 gactctgcat gtgaacgctg ctggaatccg ggtccgcaaa ggccgagcac gccagttcga
     6421 gcaacccgcc ctcgaatgca tcgaacgagg agggaccggt gatcacgggt gtgagaggcg
     6481 gacgagagtc gggatcgcgg gtcaacgaga actgatccag cggtccaagt accttgccgt
     6541 ccggcgaaaa ag
//
LOCUS       DAOQRO010000182         6538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11715_length_6538_cov_5.334413, whole genome shotgun sequence.
ACCESSION   DAOQRO010000182 DAOQRO010000000
VERSION     DAOQRO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_6538_cov_5.334413"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1530)
                     /locus_tag="PKK20_07965"
     CDS             complement(256..1530)
                     /locus_tag="PKK20_07965"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNU99863.1"
                     /translation="MTLQDAMIQIARRAKAASRELAGLSTSDKNRCLESMAAAIETHA
                     FQLETENQRDLENGAQAGLSRAMLDRLRLDDKRIAAMARGLREVAALPDPVGRLLEER
                     VRPNGLRLEKVSTPIGVIVIIYESRPNVTADAAGLCFKSGNATILRGGREALHSNRAI
                     ASIMVDAGKAALPTFPEHSIQLVQTPEREAIPALLSLSQYIDLCMPRGGEGLIRAVAD
                     CSKVPVIKHYKGVCHVFVDRAADFAMAEQIVLNAKCQRPAVCNAIETLLVDAPIAPAF
                     LPGVGKHLQQRRVALRVDEAALAILQSLLPGLRGATEQDWFTEYNDLVLNVRVVDGVD
                     AAVAHINRYGSAHSDAIVTADEARARRFLAGVDSAAVYWNASTRFTDGGEFGMGAEIG
                     ISTDKVGVRGPMGLEELTSYKWLGFGQGQLRA"
     gene            complement(1619..3829)
                     /locus_tag="PKK20_07970"
     CDS             complement(1619..3829)
                     /locus_tag="PKK20_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU99864.1"
                     /translation="MKRHLFRIGAALAVPLLLVSLAEVALRLADYGHPTSFFLRTAVH
                     GTNTLIENTWFGLRFFPPALARSPAPISFSAEKPANTYRIFLFGESAALGDPRPAYGA
                     GRWLEVLLRDRFPGREIEVVCVAMTAINSHAMPPIARSCARLDGDLWIVYAGHNEMAG
                     PFGANTVFGLQTPPRWVVRASLGAQRFRAGQLLAALIRRLGRASSPPETWGGLGMFQD
                     RHLPLDHPGRERVYRHFRQNLSDLVRIGIRSRTPILLSTAASNLRDCAPFASSLLPSA
                     IAGNAAQWDALCQEGDRLAAAGRWSEAATQFESALGLHPRSAELHFRLGQSLAALGDT
                     NAAAEGFEQARDLDTLPFRADSRINAIIEEIARDHAGERVVFTDAARALGRENPAGVP
                     GQESFYEHVHLNPEGNYRLARVWADAIVPLLEGAKTNRAAPWPEFETCARRLGLTEWN
                     RHAALEEMQRRILDALFTNQINHAVQLDHFRRQLAETRARMQAQTPSEVRLCYEEAIR
                     NRPADHWLHHNYAEYLNNSGDLAQAAAQMRQVRDLIPHHFAAHLHLGRLLARQKQFEE
                     ARESLEAALIRRPDLAEAHVELGQIAAREKQFEEALAHFDAAIQRRPKSAQVHLRRAD
                     VLMALGRREPALDSLRDATLLHPAFWEARYLLGIELAKDGKIAEARSQLSEVVRLRPH
                     HLLARLNLGNVLVRQGCVEEALTQFREVLRLDPGNQTARESVAALERHPGPENP"
     gene            complement(3887..>6538)
                     /locus_tag="PKK20_07975"
     CDS             complement(3887..>6538)
                     /locus_tag="PKK20_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU99865.1"
                     /translation="ENAAHWMDDLSIETVVAAAATPPSITANPRDQTVDEGANASFSV
                     GFDGSAPLAFQWYKNNTAIDGATAPTLTMTAVSFNDNQAKIKCEVSNTAGIATSTEAT
                     LTVVRDDTPPVLISAKGSTDFLGVVLTFSEPIDEVTGGNKDNYTIAGLTVNSATALGA
                     NVVLATTKQNEGATYIVVVNNVKDRSVAGNTIAANSKAEFKTFMFMLGRVLHKKYNGF
                     DDNSGGSPQSLFNDPRYPNQPDRMDLMPMWEYPANGASRNAAADPAREYFDTLEGYFI
                     PPEDGNYVFLTAGADRWWLYMSTDDDPANMVMIAGEPGGWSDPRGWTQIHSGNLDNRR
                     SDYSTLNQWPTAPTITLQKNKRYYMLEVHHDPSWCGADDFSATYIREFDPDPMNGSAP
                     TLTGNVVGCYVDPTGSSVTITQQPVDATQEMGRSVVFSVVATGTSTYGAALSYQWQKM
                     APGTSTWTDIAGATLASYTIPLVTLADNGTKFQVLCSVPGVTQPSATATLTVISDITA
                     PRLAGAGALPSQTGATFDVGVTFNEKVDAATAGTQSNYTLSAGTITGIKFYESSPGAV
                     LTVSGLTVGSTYTVTVRNVKDLAGNAMTSESKDFKVSAMKWGVVGANEAALGNGVLAT
                     AENGFDVYSDGVSEWGTYDEATFVYEEVTGDFDKVLRVEYQDPSSQWARAGLIARDVT
                     NFGVDRATQDGGAAGRYQKVHVNPVLTAMGTPGNNAWEGNRRLETGGTSTSAGGGGTP
                     LYPNAWCRLQRTGQTFTIFRSDDGMNWTQLGASTFDPAMPDKLFVGPEFSPENGNISD
                     EALKGVFVAKFRDYGNFSVAPPKFVSITANPDGTVTVVWTGGGTLQYTASLAPPVKWL
                     DAPAGSVSPFTFDPSQIGPTVFGRIKK"
BASE COUNT         1101 a         1988 c         2204 g         1245 t
ORIGIN      
        1 acaccattca agtccacgta cgccgccaca gcctgcaccg cgctgggttg atcctgtcac
       61 tttatttcag atgtcccagc aggcgcggcg ggcgggcagc aagaaggggc gcaggcgggt
      121 gttgcggcag ataaagaagc tggtgggaga ttaagaacct gtcactttat ctttatcgga
      181 ctcgccaaga tcgtgtagac gtgcgacggg tgttaatgtc cgtggtgatg aggctcgcga
      241 gatcggcctg gtcgctcacg cgcgcaactg gccctggccg aatcccagcc acttgtagct
      301 ggtcaactct tcgagtccca tcggaccgcg cacccccact ttgtcggtgc tgatcccgat
      361 ctccgcaccc atgccgaact cgcctccgtc ggtgaagcgc gtcgacgcgt tccagtaaac
      421 cgcggcggaa tccactccgg ccaggaaccg ccgggcccgc gcttcgtcgg cggtcacgat
      481 ggcgtccgaa tgggctgagc cgtagcgatt gatgtgggcg acggcggcgt ccacaccgtc
      541 gaccacgcgc acgttgagga cgaggtcgtt gtactccgtg aaccagtcct gctcggtcgc
      601 gccgcgcaat ccgggaagaa gcgactgcag gatcgccagg gccgcttcat ccacgcgcaa
      661 tgccacccgc cgctgctgca gatgtttccc gacgcccggc aggaaggccg gggcgatcgg
      721 ggcatcgacg agcagggttt cgatcgcgtt gcagacagcc gggcgctggc acttggcgtt
      781 gagaacaatc tgctcggcca tcgcgaagtc ggccgcgcgg tccacaaaca cgtggcagac
      841 gcctttataa tgcttgatca ccggtacctt cgagcagtcc gccaccgccc ggatcaaccc
      901 ctcgccgccc cgcggcatgc agaggtcgat gtattgactg agcgacaaca gcgccgggat
      961 ggcctcgcgc tcgggggtct gcaccaattg gatcgagtgt tccggaaacg tcggcagggc
     1021 tgccttgccc gcgtcgacca tgatggacgc gatcgcgcgg ttcgagtgca gggcttccct
     1081 gcccccgcgc aggatggttg cgttgccgga cttgaagcag agacccgcgg cgtcggcggt
     1141 cacattgggc cggctttcat agatgatcac gatcacgccg atgggtgtcg agaccttctc
     1201 gagtctcagg ccattgggtc gcacccgctc ctcgagcaat cgcccgaccg gatcgggcag
     1261 agccgcaacc tcgcggagtc cgcgggccat cgctgcgatc cgtttgtcgt ccaggcgcag
     1321 ccgatcgagc atggctcggg acaacccggc ttgggccccg ttctcgaggt cgcgctggtt
     1381 ctccgtttcg agttggaatg cgtgggtttc aatggctgcg gccatcgact cgaggcagcg
     1441 gttcttgtcg gacgtcgaca agccggccag ttcgcgcgag gcggctttgg cccgccgcgc
     1501 gatttgtatc atggcatctt gaagtgtcat aagccgatct gcaccggagc ttcgttggaa
     1561 tcctcgatcg atgaaacgcg ttgcagacgg gtcaagtgtg tatgctgcgt ctgaggggtc
     1621 aaggattctc tggaccgggg tggcgttcga gagcggcgac ggactctctg gccgtctggt
     1681 ttccgggatc caaccggagc acctcgcgaa actgcgtcag cgcctcctcg acgcacccct
     1741 gtcgcaccag gacgttgcca aggttcaaac gcgccaggag atggtgcggc cgcaggcgca
     1801 ccacctcaga cagttggctc cgcgcctcgg cgatcttgcc gtccttcgcc agctcgatac
     1861 ccaagaggta gcgggcctcc cagaacgcgg ggtgcaacag ggtggcgtcg cgaagagagt
     1921 cgagggccgg ctcccgacgc ccgagcgcca tcaacacgtc ggctcgccgc agatggactt
     1981 gagcgctttt ggggcgcctt tggattgcgg catcgaaatg cgccagcgcc tcctcgaatt
     2041 gcttttcgcg ggcggcgatt tggcccagct cgacatgggc ttcggcgagg tccggacgcc
     2101 ggatcaaggc ggcttcgagc gattccctgg cctcctcgaa ttgcttctgg cgggcgagca
     2161 ggcgtccgag atgcaggtga gccgcgaagt gatgtggaat caggtcgcgc acctgccgca
     2221 tttgggcggc ggcctgggcc aggtcgcccg agttgttgag gtactcggcg tagttgtggt
     2281 gcagccaatg gtcggcgggg cggttgcgaa tcgcttcctc gtagcaaagc cgcacttcgg
     2341 atggcgtctg ggcctgcatc cgcgcgcgtg tctcggcaag ctgtcgtcgg aaatggtcaa
     2401 gttggacggc gtggttgatc tggttggtga atagcgcgtc gaggatgcgg cgctgcattt
     2461 cctcgagtgc ggcgtggcga ttccattcgg tgagtccgag ccgccgggcg caggtctcga
     2521 actccggcca tggcgcagcc cggtttgttt tcgcgccttc gagcagcggc acgatcgcgt
     2581 cggcccagac gcgggcgagg cgatagttgc cctcgggatt caggtgaaca tgttcgtaga
     2641 acgactcctg gccggggacg cccgcggggt tctcccggcc cagagccctc gcggcatccg
     2701 tgaagacaac gcgttcgcct gcatgatcgc gggcaatctc ctcgatgatt gcgttgatcc
     2761 tcgagtctgc gcggaacgga agggtgtcga gatcgcgcgc ctgctcgaag ccttctgccg
     2821 cggcgtttgt gtcgcccaaa gcggcgaggc tttggcccag tcgaaagtgc aattcagcgg
     2881 accgcgggtg gagtccgagc gccgattcga actgagtcgc cgcttcggac cagcgtcccg
     2941 cggcggccag gcggtcgccc tcttggcaca gggcatccca ttgtgccgcg ttacccgcga
     3001 tcgcgctcgg caatagcgag gaggcgaagg gcgcgcaatc tctcaggttg ctggcggcag
     3061 tgctcaggag gatgggggtg cgggagcgga tgccgattcg gaccagatcg gagaggtttt
     3121 gccgaaagtg ccggtagact cgttcccggc ccgggtgatc gagaggcagg tgtcggtcct
     3181 gaaacatccc caatccgccc caggtctcgg gtggggaaga cgcgcgccct aggcggcgga
     3241 tgagagcggc caggagttgt cccgcgcgaa agcgttgagc gccgaggctg gcgcgcacca
     3301 cccaccgtgg cggcgtctgc aggccgaaca ccgtgttggc gccgaacgga ccggccattt
     3361 cgttgtgacc ggcgtaaacg atccacagat cgccgtcgag ccgcgcgcag gagcgggcga
     3421 tggggggcat ggcgtgggag ttgatggcgg tcatggcaac gcacaccacc tcgatttcgc
     3481 gtcccgggaa gcgatcgcgg aggaggactt cgagccaacg gcctgccccg taggccggtc
     3541 gcggatcacc gagcgccgcg gattcgccaa acagaaagat gcggtaggtg ttagccggct
     3601 tttcggctga gaacgatatg ggcgcgggac tgcgcgccag ggcgggcgga aagaagcgga
     3661 gaccgaacca ggtgttttcg atcagggtgt tggttccatg gacagcggtt cgcaagaaga
     3721 aggaggtggg gtggccatag tcggcgaggc gcagcgcaac ctcggccaga gacacaagca
     3781 gcagcggcac agccagggcc gccccgatgc gaaagaggtg tcgcttcacg caaaaaagag
     3841 gccgggggac gaccccggcc tcggtgtcac tgaaaccacg atgctcttat ttcttgatcc
     3901 ggccaaacac ggtcggcccg atttgcgacg ggtcgaacgt gaatgggctc acgctcccgg
     3961 cgggcgcatc caaccacttg acaggaggcg cgagcgatgc ggtgtattgg agcgttcccc
     4021 cgccagtcca cacgacagtc acggtcccgt cggggttggc agtgatgctg acgaacttgg
     4081 gcggggcgac gctgaagttc ccgtagtcgc ggaacttggc gacgaaaact cctttcagag
     4141 cctcatcgct gatgttgccg ttttcgggcg aaaactccgg cccgacgaag agcttgtccg
     4201 gcatggccgg gtcaaacgtg ctggcgccaa gctgcgtcca gttcattccg tcatcgctcc
     4261 ggaagatggt gaacgtctgg ccagtgcgtt ggagtcgaca ccacgcattc ggatagagcg
     4321 gcgtgccgcc gccgccggcg gatgttgagg tgcctccggt ctccaggcgc cggttgcctt
     4381 cccaagcgtt gttgcccggc gtgcccatcg cggtcagcac cgggttgaca tgcacctttt
     4441 gatagcgccc ggctgcgccg ccgtcttgcg tggcccgatc gacgccgaag ttcgtgacgt
     4501 cgcgggcgat gagacccgcg cgggcccatt gactcgatgg gtcctggtac tcgacgcgaa
     4561 gcactttgtc gaagtcgcca gtgacttcct cgtagacgaa tgtcgcttcg tcgtacgtgc
     4621 cccattcgct gacgccatcg ctgtagacgt cgaatccgtt ctcagcggtg gcgaggacgc
     4681 cgttgccgag ggcggcttcg ttggcgccca cgacgcccca tttcatcgcc gacaccttga
     4741 agtccttcga ctcgctggtc atggcgttgc cggcgaggtc cttcacgttc cgcaccgtga
     4801 cggtatacgt gctgcccacg gtcaagcccg agactgtcag gacagcgccc ggcgaacttt
     4861 cgtagaactt gatgccggtg atcgtacccg cggacagggt gtagttggac tgggtcccgg
     4921 ccgtggcggc atcgaccttc tcgttgaagg tgacgccgac atcgaatgtg gctcctgtct
     4981 ggctgggaag cgccccggcg cccgccagcc tgggcgcggt gatgtccgaa atcaccgtca
     5041 aggtggctgt cgcgctcggc tgagtgacgc ccggcaccga gcagaggacc tggaacttgg
     5101 ttccattgtc ggctagcgtc accagcggaa tggtgtacga ggccagcgtg gcgccggcga
     5161 tatcagtcca cgtgctcgtg cccggggcca tcttctgcca ctgatatgag agggctgcgc
     5221 cgtaggtgga agtccccgtc gcaaccacgc tgaagacgac gctgcggccc atctcctgag
     5281 tcgcgtcgac cggctgttga gtaatcgtga ccgagctgcc ggtcggatcc acatagcagc
     5341 cgacgacatt gccggtcagg gtcggcgccg agccattcat gggatcgggg tcgaattctc
     5401 tgatgtaagt cgcgctgaag tcgtcggcac cgcaccaact ggggtcatga tggacttcga
     5461 gcatgtagta ccgcttgttc ttctggaggg tgatggtcgg ggcggtcggc cactggttga
     5521 gcgttgaata atccgagcgg cggttatcga ggttgcccga atggatctgc gtccagccgc
     5581 gcggatcgct ccacccgccc ggctccccgg cgatcatcac catgttcgcc ggatcgtcgt
     5641 cggtgctcat gtaaagccac caacgatcgg ccccggcggt gaggaagacg tagttgccgt
     5701 cctcgggcgg gatgaagtaa ccctcgagcg tgtcgaagta ttcccgggcc gggtcggcgg
     5761 cggcgttgcg gctcgcgcca ttggccgggt actcccacat cggcatgagg tccatccggt
     5821 cgggctggtt gggatagcgg ggatcattaa acaggctttg ggggctgcca ccgctgttgt
     5881 cgtcgaagcc gttgtatttc ttgtggagca cgcggcccag catgaacatg aaggtcttga
     5941 actccgcctt gctgttcgcg gcaatcgtgt tgccagcaac cgaacggtct ttcacattgt
     6001 tcacgaccac gatgtaggtg gcgccctcgt tctgtttggt tgtcgccaaa acgacgttcg
     6061 cacccagcgc tgtcgccgaa ttcacggtca gacccgcgat ggtgtaattg tccttattgc
     6121 cgccggtgac ctcatcgatc ggctcggaga atgtcaggac cacgcccagg aaatcggtgc
     6181 tgcctttggc ggaaatcaac accggcggtg tgtcatcccg cacgacggtc aaggtcgcct
     6241 cggtcgaggt cgctattccg gcggtgttgc taacctcgca tttgatcttg gcctggttgt
     6301 cgttaaagga caccgcggtc atggtcagcg tcggggcggt ggctccgtcg atcgccgtgt
     6361 tgttcttgta ccactgaaag gccagggggg cggagccgtc gaagccgacg ctgaaggagg
     6421 cgttggcgcc ttcatcaaca gtctgatccc gaggattcgc cgtgatggac ggcggcgtcg
     6481 cggcggcggc cacgaccgtc tcaatgctca ggtcgtccat ccagtgagcc gcattctc
//
LOCUS       DAOQRO010000183         6533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11737_length_6533_cov_3.975147, whole genome shotgun sequence.
ACCESSION   DAOQRO010000183 DAOQRO010000000
VERSION     DAOQRO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_6533_cov_3.975147"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKK20_07980"
     CDS             complement(<1..601)
                     /locus_tag="PKK20_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNU99866.1"
                     /translation="MRRTFESVPGSVVAPKGFLASGVYCDIKRLGTGKGSDKGQKRDL
                     ALIVSEVPATVAGLFTTNQVCAAPVKVCIPRAAKGRAQAIVANSGNANACTGPRGLRD
                     ALEMAAVAAARIHLPAEHVLVASTGRIGVPMPMANVRDGIRRAAGLLGNAPEHGDEVA
                     EAIMTSDTRPKQIAIEMKLAGRPVRIGGVCKGAGMIQPGM"
     gene            1002..2321
                     /locus_tag="PKK20_07985"
     CDS             1002..2321
                     /locus_tag="PKK20_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNU99867.1"
                     /translation="MLNYIWLALIVLAVVLGGLEGRLDAVTDAAFDAAKTAVMTIALP
                     LIGITALWLGIMRLAEKAGLIVALARRLRPVLRRLFPDVPQEHPAMGSILMNLAANMI
                     GLTNAATPLGLRAMTDLEKLNPRPGTATNAMCTFLALNTSSVQLLPTTAIGIMAAAGS
                     TNPTAIVGTALIATVGSTAAGIFAVKTLEKLRWFRLPPPDRPPRGRSREAPNEGLGPD
                     SVEGVGNTAPAWGKLVLGVYGVFLAVAFFQLMRQTPASGETATLFLRTMNAVSVLAIP
                     FFLSFFPLYAALHRVRVYEEFVEGAKEGFHVAVRIIPFLVAMLMAIGTFRAAGGVDML
                     ARGLRPVLDLVQFPADLLPLCLTRPLSGSATIGLFADVVKTFGPDSLLARMAGTVLGS
                     TETTFYVIAVYFGSVSVRKTRHAVAAGLLADLAGIIASVVVCRIVFA"
     gene            complement(2328..3773)
                     /gene="gatB"
                     /locus_tag="PKK20_07990"
     CDS             complement(2328..3773)
                     /gene="gatB"
                     /locus_tag="PKK20_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNU99868.1"
                     /translation="MEYEAVIGLETHVQLKTQSKMWCGCANAFGAPPNTNVCPVCLGL
                     PGVLPVANDEALRLTVLGGLLLNCRIPSFAKFDRKNYFYPDVSKNYQITQYDRPSTTE
                     GHLDFEFEGAIRRVRITRAHLEEDVGKSFHFERTSGIDFNRAGVPLLEIVSEPDLASA
                     DMAYAYLNALKDVLVYGGVSDCDMEKGMVRCDVNVSVRPRGAADLGAKIEIKNMNSFS
                     GVRRALAYEIPRQIAVLERGGMLVQSTRRWDDAAGITEEMRTKEDAHDYRYFPDPDLM
                     AFEPTESWLDEVRGRVVELPLARKQRFMRDYQLPAADAQTFVDDVALGAYFEGVAGQT
                     RNPKAIANWVINNLRAHLAESQTTLADLRFRPAHLIELVELIDSGRISSRIAQDVFAE
                     FFATGESPAAIVQRKGLSQVSDAGALEALCDEAIAANPGPVADYRAGKSAALNFIKGQ
                     VMRLSKGKANPAMAGEILERKLGGNRSGTRA"
     gene            complement(3843..>6533)
                     /locus_tag="PKK20_07995"
     CDS             complement(3843..>6533)
                     /locus_tag="PKK20_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99869.1"
                     /translation="PQRYLLRFALEPQTRDRPSDTSRTEPQPMPVDSAALDRWSRHTS
                     HPDARAVRTVDQGESGGALHVTLDRLEGWETLEPPSQAWSFAPGHTLTCFEAKGSANT
                     RQLAVEWIEQDGSRWIATVDLAPQWRRYGLPPESFRPWQPPAGRGGPGDRVNLARLAR
                     FTLGLALTHTIFEPGKQEYWFANLGTAPNPFGAASAPAGLPVPRIESFAPDYMVYPVG
                     TAVTIRPPHSQTLLRPNNPDAAQTAATAIDSVVIPTGQSLVALHPRPRGVGFDQDRPY
                     RWQPILEARDLETSDFRGTVAALIAHLKPPYRDGVWALFTPGDPAFYRRLEVAGWIEQ
                     TARRLRTPVYLAEGGSQFFTLFSDQAAPIGARVANVGGRAATNLQVVLTVSERHRGSP
                     IHRHQEALSVEAHGLAIVTNAWTPGPWPEQGCVVAAQLLADNRVIDELRHDLNRWEPK
                     AAPEFLEARDGAFWIAGRRWKANGVNYMPSSGIGVVSPFFEYWLGRGAYDPEVIGRDL
                     GRIKAMGLNSISAFVYHRDLSAQHLLDLLFQCEQLGLKVNLSLRPGTPMDFRWDEMRA
                     LIEHYRLARNDTVIAYDLAWESSHYDQRHQQRHYTLAWAEWVRRRHGSTAQALEAWGE
                     PNANARPSTPSPNTTPDVPVPAMSELTQDGPWRKRIADYRAFLDELLADKYAEARRLV
                     RSIDPNHLVSFRMQHAGDPTYNAEGMLPYDFYGLRDAVDIWEPEAYGRIGDWERVKPG
                     HFTAAYARLCDPAKPVLWSEMGYSVWDSQRRAPAPTRLEFAARYYRDFYRMLRESGVD
                     GVFFWWYPGGYRANENSDFGIIHPDGTDRPVTRVIRAEGPHFLAAPPVPAVTRWIEVD
                     RDRDARGLHGIYEAAKAEYWSIVETGGQAGLRWKRPPGGR"
BASE COUNT         1051 a         2045 c         2201 g         1236 t
ORIGIN      
        1 tcatgcccgg ctggatcatg cccgcgccct tgcagacacc tccgatccgg acaggccgtc
       61 cggcgagttt catctcgatc gcgatctgtt tgggtcgggt gtcgctggtc atgatggcct
      121 cggcgacctc atcgccgtgt tccggggcat tgccgaggag cccggcggcc cgccgaatcc
      181 catcgcgcac gttggccatc ggcatcggca cgccgatgcg tccagtcgag gccaccagga
      241 catgctcggc cggcaggtgg attcgtgctg ccgcgacggc agccatttcg agcgcatccc
      301 gcaggccgcg cgggccagtg catgcgttgg cgttgcccga gttggccacg atagcttgcg
      361 cgcggccttt cgctgcgcgc gggatgcaga ccttgaccgg cgcggcgcaa acctggttgg
      421 tggtaaacag ccccgctacc gttgccggca cctcggacac gatcaatgca agatcgcgtt
      481 tctgtccttt gtccgaccct ttcccggtgc cgaggcgctt gatgtcacag tagaccccgc
      541 tggccagaaa acccttcgga gcgaccaccg aacccggaac gctttcaaaa gtccttctca
      601 tcgttgggat cgatgctgtg gcatcacagg cgttttggaa aggcggttgt gcgatcatgg
      661 gttggcgcac gctcggggtg cccattgccc tcagcaatgt tctccagagc agctaggacc
      721 ggtgcatgcc ctcgcctccg tggctactgg acgtgctccc tgcactggtg cgggcgctga
      781 gcgccatgtt gtcgcctgga ggaaaggaga accacgaatc agacgaatct cgcgaatggc
      841 agagacggct gcaagcgcac gtgcaaggag gattcggcca attgactaac ggaatgtcga
      901 gaacggagga ggcggaggcc gtgtgcggat tcgtgggctc tggatcgtcg cgtcgttcac
      961 ttctgcgttg cccatcgggt cagagtgtgt aaaccacgcc catgctcaac tacatctggc
     1021 ttgcgttgat cgtgctcgcc gttgtcctcg gcggcctcga ggggcgcctg gacgcggtga
     1081 ccgatgcggc cttcgacgcg gccaagaccg cggtgatgac catcgcgctg ccgctcattg
     1141 gcatcaccgc cttgtggttg gggattatgc ggttggcgga gaaggcgggt ctgatcgtgg
     1201 cgctggcgcg gcgtctgcga cccgtgctgc gccggctgtt tccagacgta ccgcaggagc
     1261 accccgcgat gggctcgatc ctcatgaacc tggccgcaaa catgatcggc ctgaccaacg
     1321 ccgccacgcc gcttggtctg cgcgcgatga ccgacctcga gaagctcaat ccccgccccg
     1381 gcaccgccac caacgcgatg tgcaccttcc tggctctgaa caccagctcg gtccaacttc
     1441 tgccaacaac cgccatcggc atcatggccg cggcaggttc gaccaacccg accgccatcg
     1501 tgggcaccgc cctcatcgcc acagtcggct cgacggcggc tggaatcttc gccgtcaaga
     1561 cactcgaaaa gctgcgctgg ttccgtttgc cacctccgga tcggcctccc aggggccggt
     1621 cccgcgaggc gccgaatgag ggtctcggac cggactcggt cgagggcgtg ggcaacacgg
     1681 ctcctgcgtg gggcaagctg gttctggggg tctacggcgt tttcctggcg gtggcattct
     1741 ttcagttgat gcgccagacg ccggcatccg gtgaaaccgc cacgcttttc ttgcggacca
     1801 tgaacgccgt gtcggttctc gccatcccgt tcttcctgtc gttcttcccc ctctatgccg
     1861 cactgcaccg ggtgcgggtg tacgaggaat tcgtcgaggg cgccaaggag ggtttccatg
     1921 tggcggtgcg catcatcccg ttcctggtcg ccatgctgat ggcgatcggg acattccgcg
     1981 ccgcaggcgg cgtggatatg ctggctcggg ggctgcgtcc ggtcctcgat ctggtgcagt
     2041 ttcccgccga cctgctgccg ctctgcctga cgcgtccgct gagcggaagc gcgacgatcg
     2101 gcctctttgc cgatgtggtg aaaacgttcg gtcccgacag tctcctggcg cgtatggccg
     2161 ggacggtttt gggaagcacc gaaacgacgt tctacgtcat cgccgtgtat ttcggttcgg
     2221 tgtccgtgcg caagacgcgc catgcggttg cggcggggct cctggccgat ttggcgggga
     2281 tcatcgcgtc ggtcgtggtc tgtcgaatcg tgtttgcgtg aaccggttca cgctcgggtg
     2341 ccggaccggt tgccgcccaa cttccgttcg agaatctcac ccgccatggc cgggttcgcc
     2401 ttgcctttgc tcaatctcat gacctggccc ttgatgaagt tgagcgcggc gctcttgccc
     2461 gcgcggtagt ccgcgaccgg gccgggattg gccgcgatgg cctcatcgca gagggcttcg
     2521 agggcgccgg catcgctgac ctggctcagg cccttgcgct ggacgatggc ggcggggctc
     2581 tctccggtgg cgaagaactc ggcaaagacg tcctgggcga tccggctgct gatcctgccg
     2641 ctgtcgatca attctaccag ttcgatcaga tgcgccggcc tgaacctcag atcggcaagt
     2701 gtggtttgcg attcagccag gtgtgcgcgc agattgttga tcacccagtt ggcgattgcc
     2761 ttgggattgc gcgtctgccc cgccacaccc tcgaaatagg cgccgagcgc cacgtcatcg
     2821 acaaatgtct gcgcgtcggc ggccggcagt tggtagtcgc gcatgaaacg ctgcttgcgc
     2881 gccagcggca gctcgacaac ccgcccccgc acttcgtcaa gccatgactc cgtcggctcg
     2941 aatgccatca ggtccggatc gggaaagtac cggtagtcat gcgcatcctc cttggtccgc
     3001 atttcctcgg tgatcccggc ggcgtcgtcc catcggcgcg tggactggac aagcatgcct
     3061 ccgcgctcga ggacagcgat ctgacgcgga atctcgtatg cgagagcgcg ccggactccg
     3121 ctgaaactgt tcatattctt gatctcgatc ttcgccccga ggtcggcggc gccgcgcgga
     3181 cgaacgctga cattgacgtc gcatcggacc atgccttttt ccatgtcgca gtcgctgacg
     3241 ccgccataga cgaggacgtc tttgagcgcg ttcagatagg cgtaggccat gtccgcactg
     3301 gccagatccg gttcagacac gatctcgagc aggggcacac ctgcgcggtt gaagtcgatc
     3361 ccgctcgtcc gctcgaaatg aaaactcttg cccacgtcct cttcgaggtg agcgcgcgtg
     3421 atgcgaacgc ggcggatggc gccctcgaac tcgaagtcca gatgcccttc ggttgtcgag
     3481 ggccggtcgt attgtgtgat ctggtagttc ttggacacgt cgggatagaa gtagttcttc
     3541 cggtcgaact tggcgaagga ggggattctg cagttgagca gcaggccccc cagcacggtg
     3601 aggcgcaggg cctcatcgtt ggcaaccggc agcacgcccg gcaacccgag acagacgggg
     3661 cagacgttcg tgttcggcgg cgcgccaaag gcattggcgc aaccgcacca catcttcgat
     3721 tgggtcttga gctggacatg tgtctccagc ccgatcacgg cttcgtactc catagggcca
     3781 cacggttttg ccacaaccgc caacgacttt gcactcaatt ctatctcaac ccgatatgcg
     3841 ggctaacgcc cgccgggcgg tcgtttccat ctcaggccgg cttggccgcc cgtctcgacg
     3901 atgctccagt actcggcttt ggcggcttcg tagattccgt gcaggccgcg cgcgtcccga
     3961 tcgcggtcca cctcgatcca gcgtgtgacc gccggcacgg gtggagcggc gaggaagtgg
     4021 gggccctcgg cgcggatgac gcgggtgacg gggcggtcgg tgccgtcggg gtggatgatg
     4081 ccgaaatcgc tgttttcatt ggcccggtag cctccggggt accaccagaa gaaaacgccg
     4141 tccacgcccg actcgcggag catgcggtag aagtcccggt aatatcgcgc ggcgaactcg
     4201 agcctggttg gggcgggcgc gcggcgttgg gaatcccaga cgctgtagcc catttcggac
     4261 cagaggaccg gtttggccgg atcgcacagg cgggcgtagg cggccgtgaa gtggcccggc
     4321 ttgacgcgct cccagtcgcc aatgcgtccg tatgcctccg gttcccagat gtccacggcg
     4381 tcacgcaagc cgtagaaatc gtaaggcagc attccctcgg cattgtaggt ggggtcgccc
     4441 gcgtgctgca ttcggaaact gacgaggtga ttggggtcga tcgatcggac caggcggcgc
     4501 gcctcggcgt acttgtcggc cagcagttca tcgaggaagg cgcggtaatc ggcgatgcgt
     4561 ttgcgccacg ggccgtcctg ggtgagttcg gacatcgcag gcacgggcac gtccggggtg
     4621 gtgttggggc tcggtgtcga gggacgggcg tttgcgtttg gttcgcccca ggcttcgagg
     4681 gcctgagcgg tggaaccgtg gcgacggcgc acccattccg cccaggctag cgtgtagtgg
     4741 cgttgctgat ggcgctgatc gtagtggctc gattcccaag cgaggtcgta ggcgatgacc
     4801 gtgtcgttgc gcgcaaggcg gtaatgctcg atgagggcgc gcatttcgtc ccatcggaag
     4861 tccatgggcg tgccgggccg cagcgagaga ttgaccttga gaccgagctg ttcgcattgg
     4921 aagagcagat cgagcaggtg ctgggcgctg aggtcgcggt gatagacaaa cgcgctgatg
     4981 gaattgagac ccatcgcctt gatgcgcccc aggtcgcgtc cgatcacctc gggatcgtat
     5041 gccccgcggc ccagccagta ctcgaaaaac gggctgacga cgccgatgcc cgaggagggc
     5101 atgtaattca caccattggc cttccaccgc cgccccgcga tccagaaggc gccgtcgcgc
     5161 gcttcgagga actcgggcgc ggccttcggt tcccagcggt tcaaatcgtg gcgcaactcg
     5221 tcaatgaccc tattgtccgc cagcagttgg gccgcaacga cgcagccttg ctcgggccac
     5281 ggacccgggg tccatgcgtt tgtcacaatc gccagcccgt gcgcctcgac gctgagcgcc
     5341 tcctgatgac ggtggatcgg cgatccgcgg tgtctctcgc tcacggtcag gacgacttgc
     5401 aggttcgtgg cggcgcgccc gcccacattc gccacgcgcg caccgatcgg cgccgcctga
     5461 tctgaaaaca gggtgaagaa ctgactgccg ccttcggcca ggtaaacggg tgtgcgcagc
     5521 cggcgcgccg tctgctcgat ccagccggcg acctcgagtc ggcgatagaa ggcgggatcg
     5581 cccggggtga agagcgccca gacaccgtca cgatacggcg gtttgaggtg cgcaatcagt
     5641 gccgccacgg ttccccggaa gtccgaggtc tcgaggtccc gcgcttcgag aatgggctgc
     5701 cagcgatagg ggcgatcctg gtcaaagccg acaccacgcg ggcgcggatg cagcgctacc
     5761 agcgattggc cggtagggat gacgacgcta tcgatcgccg tcgcggcggt ttgagcggca
     5821 tccgggttgt tagggcggag caatgtctgc gagtgcggcg ggcggattgt caccgcggtg
     5881 ccgaccgggt agaccatgta gtccggagcg aaggattcga ttcggggcac gggaagacct
     5941 gcgggggcgg atgcggcgcc gaagggattt ggcgcggtgc ccagattggc gaaccaatac
     6001 tcctgctttc cgggttcgaa gatcgtgtgt gtgagcgcca ggcccagagt gaagcgcgcc
     6061 aaccgggcga ggttgacccg gtcgcccggg cctcctcggc ccgctggagg ctgccaaggt
     6121 cgaaacgact cgggaggcag accgtagcgg cgccattggg gggcgaggtc gacagtggcg
     6181 atccaacgcg agccgtcctg ctcgatccac tcgacagcca gttgacgggt gttggcgctg
     6241 cctttggcct cgaaacaggt gagggtatga ccgggcgcaa aggaccacgc ctgggacggc
     6301 ggttcgagag tctcccagcc ctcgaggcga tccaaggtga cgtgcagcgc gccgccgctt
     6361 tccccctgat ccaccgtgcg cacggcgcgc gcgtccgggt ggctggtgtg ccggctccaa
     6421 cggtccagtg cggcggaatc gaccggcatg ggttgcggct cggtgcggga tgtgtcggac
     6481 ggccggtcac gggtctgggg ttcgagcgcg aaccggagca agtaccgctg ggg
//
LOCUS       DAOQRO010000184         6469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_11891_length_6469_cov_4.186155, whole genome shotgun sequence.
ACCESSION   DAOQRO010000184 DAOQRO010000000
VERSION     DAOQRO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11891_length_6469_cov_4.186155"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..2615)
                     /locus_tag="PKK20_08000"
     CDS             complement(138..2615)
                     /locus_tag="PKK20_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99870.1"
                     /translation="MRALDAQVVYEQVHSFGASADSGAYPNATPLIGLDGRLYGSTLY
                     GGSAGAGTLYRVERNGSDFVVLHNFSGFDGSRPIGGLAQDEAGVLYGAAAGGGYGTNG
                     VVFKINPDGSDFAVIKELAADGSEGREPAGGLVRASNGVLYGATFSGGAYDGGTIFSV
                     NTDGTEFTVLRELGDSPADARGPRCFLIEGSDGMLYGTTRYGGTEDAGTLFRITKFSF
                     GYEVLKSFVPATEGQGPLAGVIEGRDGRLYGTLGEYGLGDAGAVYAINPDGTGFTILR
                     SLVPSEGAGILAGVCQTSDGMLYVIASSEGANLKGSVMRFREDGSEFSVVQDFGTSPG
                     SPSTGITALVEGPDGQLYYTTALGGAFDDGTVARMQKDGSGLQILRSFSESGSAAITP
                     REGVVVGKDGILYGTTVEGGINNLGTVYRIRADGSGYGVLRVLGQSQSDAALPVAGLI
                     QASDGFLYGTATAGGTHGQGALFRVAPDAGGYAVVYSFRGKPADGAVPSTKLLEAKDG
                     FLYGTTSYGGSSNNGTIFRIRKDGTEYTVLRSFGGAPNDASNPNGDLLQTSEGLIYGV
                     TYIGGSANLGTVFRLNPDGSGYSVVRSFTGALQTPANPWSGLIEGSDGRLYGTSLATG
                     GNGGSVYSLAKDGTDLRVIVALPKAVDGGTRPLGRLVEDSTGQLWGICWLGGPANGGT
                     VFKVAKDGSGYQVAHEFLGAPADGFAPISSLAIFSDGVIYGTTTSGGTLDFGSLFRIS
                     SSHAVHIEGVSKQPDGPATVSISAVPGWLCQLEWTSEVRPSAWNSVVTGRANAAGKLV
                     LTDVAANDQAIRFYRVRATPSTADQAN"
     gene            complement(2904..>6469)
                     /locus_tag="PKK20_08005"
     CDS             complement(2904..>6469)
                     /locus_tag="PKK20_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNU99871.1"
                     /translation="DREGRLKSPSAWGTVWEDLVELEQHHPGLRRHFGDVFLEWCRRG
                     VDGFRCDAGYQVPLPVWQYVTARVRQVFPDTVFLLEGLGGAWETTESLLTEGGLQWAY
                     SELFQNYDGVQVAGYLDHALRQSQRVGLLVHYSETHDNERLAAKGRAWSLLRNRLCAL
                     ASVNGAFGFTCGVEWLAPERVNVHSSRGLAWDSADNIVSELSVLNRLLADHPCFFDGA
                     RLTRLSEPGSPVYGLRRVSAEGLDEVLVLANTDVASPQPLRLHADSLPSGANGGSALV
                     DLLDQEPPDFALKGANLIVTLPPAAVFCLASSRGPRGLAGEAYRRRKACEARALSTLC
                     HHLPVEMLGAIDVVSLGKRFSENPAAYLSALTYLTPATAGQDVESALDAATTAFPRVV
                     TWEWPERNRVVPVPPEHWLLVRDDAPFRATLAASPPTASVPQHAVSIPTAQGHIACFA
                     PRGDIGDASLTVDRFVNEADRRAEAPVRFLPARPHEGERSALPAPDSVVLLTNERGGM
                     ARLRVDLGSIESKYDCLLAANLHPRVPVDRHVFAKRARVWINADGFISPLDAGNLLAF
                     SHGPPARWRFLAHAGDGREVEIGLEVGMIPGRNTTVLRFSRVDGAGSDRKSLPPEADV
                     RLTVRVDIEDRGFHAETKRGSGVEGHFAKHCQSLAAHPTQSGTRSRACRPDLSDPAHS
                     NAPSGRSGFVFTPTADRELRVWTDAGEYHPQPEWCESIPHPIEQSRGQIGVGDAFSPG
                     WFELCLDPGRAVALVVCADPRDPTPEEVLGPAQREQRQAGFEERLAAAAGAFVVRRDA
                     VRTVIAGYPWFLDWGRDTLICARGLLAAGMRETVIHLLQAFGRFEEHGTLPNAIHGED
                     ASNRDTSDAPLWYGIVCEELAAGDSEMLHALRVDAGGRTIADVLRSIAVGYARGTPNG
                     IRMDPASALIWSPSHFTWMDTNHPPGTPREGYPVEIQALWIRLLRHLDRLRLPPAAEP
                     WDQLARRAQSSLSKHFWLEDQGWPADVLEAGPRQSAASATPQRALRPNCLFSISFGLI
                     AGEQARRTAEAARKHLLVPGALRSLAPLPVDPPLAIHGPDGRTLNDPKHPYWGRYAGD
                     EDTQRKPAYHNGTAWVWMLPIFCEAMVRAWDCAPGAQAAAQAYLGGSESLLDRGCLGH
                     LPEILDGDAPHRQRGCDAQAWSVTEALRVWRWLDALACSPG"
BASE COUNT         1290 a         2112 c         1974 g         1093 t
ORIGIN      
        1 atggcatgca cggcctccaa gacgcggtga gccagacccc actggacaaa ccaattagca
       61 ctttggcagt cgctcgatgc cactgccaga atggtgggtg gccagaatgg ttctggggcc
      121 gccccaaaaa agagactcta attcgcctgg tctgcggtcg agggtgtagc acgcacgcga
      181 tagaatctga tcgcctgatc gtttgcagca acgtcagtca gaacgagttt tccggccgca
      241 ttggctcgac cggttaccac gctgttccag gcagacggtc tcacctccga agtccattca
      301 agttggcaca gccatccagg aactgcgctg atgctgacgg ttgctggacc gtctggctgc
      361 ttcgagacgc cttcgatatg gacagcatgg ctcgaggaaa tgcgaaagag gctgccaaag
      421 tcgagagtac caccgctcgt cgtcgttcca tagatgacgc cgtcggagaa gatggcgaga
      481 ctggagattg gcgcgaagcc gtccgcgggg gccccaagga attcgtgcgc gacttggtat
      541 ccggacccgt ctttggcaac tttgaagaca gtgccgccat ttgccgggcc accgagccaa
      601 cagatacccc aaagctgtcc ggtcgaatcc tcgacaagcc gtcctagagg gcgcgtgcct
      661 ccgtcgactg ccttgggcaa agcaacaatg accctaaggt cagttccatc cttggccagg
      721 gaatagacac ttccaccgtt tccacccgta gccaggctgg tcccgtacag acgcccatca
      781 ctcccttcga tcagcccgct ccacggattt gccggcgtct gtaacgcgcc tgtgaaggac
      841 ctcacaaccg agtatccgga gccgtccgga ttgaggcgga acacagtgcc aagattggcg
      901 ctgccaccaa tgtatgtaac tccgtaaatg agcccttcag atgtttgcag cagatccccg
      961 ttggggttgc ttgcatcgtt gggagccccg ccgaacgacc tcagcaccgt gtattccgtg
     1021 ccgtccttgc gaatgcggaa gattgtgccg ttgttcgagg aacccccgta ggatgtggtg
     1081 ccgtaaagga aaccatcctt ggcttcgagc agcttggttg atggcactgc accatcggca
     1141 ggcttccctc tgaaactgta aaccacagca tagccaccgg catccggcgc gacccgaaac
     1201 agggcacctt ggccatgtgt gcctcccgca gtggctgttc cgtaaagaaa gccgtcgctt
     1261 gcctggataa gacccgcgac cggcaacgct gcgtcgcttt gagactgacc gagaacgcgg
     1321 aggacgccgt aacccgaccc gtccgcccga attctataga cggtcccaag attgttaatc
     1381 cccccttcca cggtcgtgcc gtagaggatt ccgtccttcc caaccacgac gccttcgcga
     1441 ggtgtgatgg cagcggaacc actctcggag aagctcctga gaatctgcaa gccgctccca
     1501 tccttctgca ttcgagcgac tgtgccatca tcgaacgcgc cgccgagtgc agtcgtgtag
     1561 tacagttgcc catccggccc ctcgacaagc gctgtgatgc ctgtgcttgg gcttccagga
     1621 ctcgttccga agtcctggac gacggaaaac tccgagccgt cctctctgaa tcgcatcacc
     1681 gagcctttaa ggttcgctcc ctcagacgaa gcgatcacgt agagcattcc atcgctggtc
     1741 tgacaaacgc cggcaagaat ccctgctcct tcactcggaa ctaaggagcg cagaatcgta
     1801 aagccagtgc cgtcagggtt gatggcgtac actgcacctg cgtcgcccag gccatactct
     1861 cccaaagtgc catagagccg tccgtccctt ccctcgatca cccctgcgag aggcccttgg
     1921 ccttccgttg cgggaacgaa gctcttcaga acttcgtaac caaagctgaa cttcgtgatc
     1981 cgaaataggg tgcccgcatc ctctgtaccg ccgtaacggg ttgtcccata aagcattcca
     2041 tccgaccctt caatcaggaa gcaccgaggt ccccgcgcgt ccgcggggct gtcaccgagt
     2101 tcccgcagaa ccgtgaactc agtgccgtcg gtattgacgc tgaaaatcgt tcctccgtcg
     2161 taggcacccc ctgaaaaggt tgcgccgtag agcacaccgt tgctggcccg cacgagtccg
     2221 ccagcgggct cgcggccctc cgaaccatcc gcggccaatt ccttgatgac agcgaaatcc
     2281 gagccgtcag gattgatctt aaaaacgaca ccgttggtgc cgtatccgcc accggctgcc
     2341 gcgccataga gaacccccgc ctcgtcttgc gccaacccgc caatggggcg actgccatcg
     2401 aatccactga agttatgaag aaccacaaaa tccgaaccgt ttcgctcgac tctgtagagc
     2461 gtgccggcgc cggctgatcc accgtagagg gtcgatccat agaggcgccc atccaaaccg
     2521 atcagcggag tcgcgtttgg atacgccccg ctgtcggcac tcgcgccaaa cgaatggacc
     2581 tgttcgtaaa caacttgggc atccagcgca cgcatgccca agagcagcaa tgacgtccaa
     2641 ataaggcacg gccttaggaa acgcttgtca aacgagggga gcagcatggt tttatgcatg
     2701 gcaggattgt gtcttacggc tcagatcact tacatttaca agcgcttctg atatccggat
     2761 ttggcccctc ttgtaaagga caatctcagc aaaaaaaacg cgacccgcga atcctcaaac
     2821 tccgccacct catgctcacc gcaccgtccg gactgtcaat ccggtgtcgg tatgcacgtc
     2881 ccccgcctcg gtgccggcaa ggatcagccc ggagagcagg cgagcgcatc caaccaacgc
     2941 cagacacgca gggcctcggt cacactccag gcttgggcgt cacagccccg ctgcctgtgg
     3001 ggcgcgtcgc cgtccagaat ttcgggcaga tggcccaggc agcctcgatc gagcaacgat
     3061 tccgagccgc ccaaataagc ctgggcggca gcctgggcgc cgggcgcaca gtcccaagcc
     3121 cggaccatgg cttcgcagaa gatgggcaac atccataccc aggccgtgcc gttatggtaa
     3181 gcgggcttgc gctgggtgtc ttcgtctccg gcgtagcgcc cccagtaggg atgcttcgga
     3241 tcattcaggg tgcggccatc gggaccgtga attgcgagcg gggggtcaac gggcagcggc
     3301 gctagggatc gcaacgcgcc cggcacaagc agatgcttgc gggcggcttc cgcggtgcgt
     3361 cgggcctgct cgcctgcgat caatccaaat gagatggaga acaggcaatt ggggcgaagc
     3421 gcgcgctgcg gggtggcgga cgcggcgctc tggcgcggcc cggcctcgag gacatccgcc
     3481 ggccatccct ggtcctcgag ccagaagtgc ttcgagagtg aggactgggc gcgccgggcg
     3541 agctgatccc agggctcggc ggcgggcggc aacctcagcc gatcgaggtg ccggagaaga
     3601 cgaatccaga gggcttgtat ttcgaccggg tacccctcgc ggggcgtgcc gggcgggtgg
     3661 ttggtgtcca tccacgtgaa atggctcgga ctccagatca gggccgaggc cgggtccatg
     3721 cggattccgt tcggagtgcc ccgcgcatag ccgacagcga tcgaacggag cacatccgcg
     3781 atcgtgcgtc cgcccgcatc cacgcgcagc gcatggagca tttccgaatc gcccgcggct
     3841 aactcctcgc aaacaatgcc ataccagagg ggcgcgtcgg aggtgtcgcg attcgacgcg
     3901 tcctcgccgt ggattgcatt ggggagagtg ccatgctctt cgaagcggcc gaaggcctgc
     3961 agcagatgaa tcacggtttc tcgcatcccg gccgccaaaa ggccccgtgc gcagatgagc
     4021 gtgtcgcggc cccaatccag gaaccatgga tagcccgcaa tcactgtgcg gacagcatct
     4081 cggcggacaa cgaacgcgcc cgcggcagca gccaggcgct cctcgaatcc cgcctgcctc
     4141 tgctcccgct gggcagggcc cagcacctcc tccggggtcg gatccctggg gtcggcgcaa
     4201 acaacaagag ccacggcacg acccggatcg aggcacagtt cgaaccaacc cggactgaac
     4261 gcgtcgccga cgccaatctg cccgcgcgat tgctcgatgg gatgcggaat actctcgcac
     4321 cactcgggct gcggatggta ctcgccggcg tcggtccaaa cccgcagttc ccggtccgcg
     4381 gtcggcgtga aaacgaaacc ggaccgaccg gacggggcat tcgagtgcgc gggatcggag
     4441 agatccggac ggcacgcgcg cgatcgagtg ccggactgcg tcggatgcgc agcgagagac
     4501 tggcagtgtt tggcgaagtg gccctcgacc ccgctgccgc gcttcgtctc cgcgtggaat
     4561 cccctgtcct cgatatcgac gcgcacggtc aaacggacat cggcttccgg gggcaggctc
     4621 ttgcggtccg accccgcccc gtcgacccgg ctgaaccgga gcacagtggt gttgcgaccg
     4681 ggaatcatcc cgacttcgag gccgatctcg acctcacgtc cgtctccggc gtgggcgagg
     4741 aaccgccacc gagcgggggg accgtgcgag aaggccaaca gattgccggc atcgaggggc
     4801 gaaatgaaac cgtcggcatt gatccagact cgagcccgct tggcgaagac atggcgatcc
     4861 accggaacgc gcgggtgcag gtttgccgcg agcaggcagt cgtacttgga ttcgatcgaa
     4921 ccgaggtcga cacgcaggcg cgccatgcca ccgcgctcgt tggtgagcaa gaccacggaa
     4981 tcgggcgcgg gtagtgccga ccgttcgccc tcatgcggtc tcgccggcag aaaacgcact
     5041 ggggcctcgg cgcggcggtc cgcttcgttc acgaatcgat cgaccgttag actcgcatcg
     5101 ccgatatcgc ctcggggcgc aaagcaggcg atgtgcccct gggcggttgg gatggacacc
     5161 gcgtgctgcg ggaccgatgc ggtcggcggg ctggcagcca gcgttgcgcg aaagggagcg
     5221 tcatcacgga ccaggagcca atgctccggc ggcacgggca cgacacgatt ccgttccggc
     5281 cactcccagg tcaccaccct cgggaatgcg gtcgtcgccg catcaagagc ggattcgaca
     5341 tcctggcctg ccgtagcggg cgtcaggtaa gtgagcgcgc tcaagtaggc cgcggggttc
     5401 tccgagaagc gcttccccag cgagaccacg tcgatggcgc cgagcatctc gaccggcagg
     5461 tggtggcaca gcgtgctgag cgcccgtgct tcgcacgcct tgcggcgccg ataggcctcg
     5521 cccgccaagc cacgcgggcc ccggctggac gccagacaaa agacggctgc cggcggcagg
     5581 gtcacgatca ggttcgcgcc tttgagggcg aaatccggag gctcctgatc cagcagatcg
     5641 accaacgccg atccaccgtt agcgccggac ggcaggctgt ctgcatgaag ccggaggggc
     5701 tgcggcgacg cgacgtcggt gttcgccagg acaaggacct catcaaggcc ttccgcggag
     5761 acccgccgca agccgtagac gggcgagcct ggctcgctca agcgagtcag ccgggccccg
     5821 tcgaagaagc acggatgatc ggccaacaac cggttcagca ccgacagctc cgacacgata
     5881 ttgtccgcgc tgtcccaggc caagccacgg cttgaatgga cattcacgcg ctctggagcg
     5941 agccattcaa cgccgcaggt gaaaccgaag gcgccgttga cactggcaag ggcgcagaga
     6001 cgattgcgca ggagggacca ggcgcgtcct ttggccgcaa gccgctcgtt gtcatgggtt
     6061 tcgctgtagt ggacaagaag cccgacgcgc tgactctggc gcaatgcatg gtccaggtac
     6121 ccggccacct ggacgccatc atagttctga aacaactccg aataggccca ttggagaccg
     6181 ccctcggtca ggaggctctc ggtcgtctcc caggcgcctc ccagcccctc gagcaagaag
     6241 acggtgtccg ggaacacctg gcgcacacgg gcggtcacgt actgccagac cggcagcgga
     6301 acctggtacc ccgcatcgca gcggaagcca tccacacccc gccgacacca ttcgagaaag
     6361 acatccccaa aatgacggcg cagtcctggg tgatgctgtt cgagctcgac gaggtcttcc
     6421 caaacggtcc cccaggcgct ggggctcttc aaccggcctt cccgatccc
//
LOCUS       DAOQRO010000185         6403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12080_length_6403_cov_4.712980, whole genome shotgun sequence.
ACCESSION   DAOQRO010000185 DAOQRO010000000
VERSION     DAOQRO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12080_length_6403_cov_4.712980"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK20_08010"
     CDS             complement(<1..669)
                     /locus_tag="PKK20_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNU99872.1"
                     /translation="MSNSANNYPKLHNAMWPGLVGKGGPGAEPGIDLDTMLDLTAKAE
                     ANGVKFDGVDLFLSAPHIDIDSTDDDLKRVADKIAGKGLAIGSVVAPVWKGTGGGSAM
                     GGEEERKKFVGQVRKGCRIASAFRKLGLRSGGVVRIDSSASVTEWDKDPKGNTKRIIE
                     TFKEAAKVAKGCGERLAAEGEICWGGMHSWKYMLQVLEGVGQPRVLGFQADMAHTLLY
                     LLGSN"
     gene            918..2153
                     /locus_tag="PKK20_08015"
     CDS             918..2153
                     /locus_tag="PKK20_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNU99873.1"
                     /translation="MDIRKIGLTMLGVLATGVWAQQPITEAETNALAAIRAQRTDGLI
                     TVQPWVQLLDSNRLGVGWITSQPSDGAVEWTQSENGGEWRQAWFSEDGLRQANTAMQR
                     AVIGGFDPAKPIRFRARSRAIESFKPYSVAFGQPTVSQDRSLPARSRPNGAVSFLVFN
                     DIHNRAQLYPLLVEKAGMPVDFAVFNGDVLNDPQTESEVVDNLLMPMAWFAAKSIPCF
                     FLRGNHETRGAFARRLKDYLILPGERYYAAMTFGSARVVFLDTGEDKPDTSKEYSGLV
                     DFDPYIEAQTDWLRREIAGDAFQQATWRLVVMHMPPDWRMDEAKLWHGQRRVNERFAP
                     LLDAGKVTAVISGHTHAPEVVEPCPDASRGFQWPVFIGGAPSLAKATVFRVDADATTL
                     KITRFASDGTVGAERTWKK"
     gene            2280..3599
                     /locus_tag="PKK20_08020"
     CDS             2280..3599
                     /locus_tag="PKK20_08020"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU99874.1"
                     /translation="MSAPISQSPTQVVSVPQEVTGQPRRVVVGRVTWRDTFAALRYRN
                     YRLFFVGQLVSLIGSWMHNTAQGWLVYQLTGSKVLLGTVAAVGSLPMLLLSMWGGSVA
                     DRHPKRVVICCTQIAMMLLAFAFAALVWSGKVQPWHILVLAALDGAAMAFDMPARQAF
                     MVEITARDDLMNAVSLNSSIVNGARVIGPAVAGFLMANVGMTLCFVLNGISYFAVIAG
                     LLMMRLPKFVPPPQPASAGRHILDGFAYAAAHRRLRALFLLLGVVGIFGWSYSVLLPA
                     YATDILGVSESGYGALLSANGLGALLGALTVATFGSRMQPRLLILGGLWTFSAMLLLL
                     AFVRWLPLVLLCLAVGGWGLLLYFATTNTLIQTSVSDAMRGRVMGIWGLVFGGMVPIG
                     GLESGVLSQAVGVPWTITVGAAVCAGAGLVAWWQGRSQPGPSLNPRP"
     gene            3927..5033
                     /gene="hypD"
                     /locus_tag="PKK20_08025"
     CDS             3927..5033
                     /gene="hypD"
                     /locus_tag="PKK20_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNU99875.1"
                     /translation="MKFIDEYRDADRAQECARAIARSVTRPWTIMEVCGGQTHAIVKF
                     GIDELLPRDITLVHGPGCPVCVTPLELIDKAIAIASKPGVIFCSFGDMLRVPGSHTDL
                     LSVKASGGDVRMVYSPMDSVRLAREHRDRQVVFFAVGFETTAPANAMAAFQARREQLT
                     NFSLLVSHALVPPAISTVLGAPHNRVQAFLAAGHVCAVMGYHQYEPLAREYRVPIVVT
                     GFEPIDILQGIRRCVLQLEAGEAKVENQYARAVKREGNLPAQHMVREVFQVIDRKWRG
                     IGVIPRSGFGLSPAYAEFDAERRFGVADYHADEPSECLSGLVLQGVRKPVDCPAFGTR
                     CTPEHPLGATMVSNEGACAAYYRYRRQQEPHRSL"
     gene            5030..6079
                     /gene="hypE"
                     /locus_tag="PKK20_08030"
     CDS             5030..6079
                     /gene="hypE"
                     /locus_tag="PKK20_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNU99876.1"
                     /translation="MTSDVTAACPLPIGQYTHVLMAHGGGGRLMQQLIDQVFISAFSN
                     PRLDERHDGAILDAQGRRFAFTTDSYVVRPLFFPGGDIGTLAVNGTVNDLAMCGARPR
                     WLSAGFILEEGLPIETLERIVASMRQAAATAGVEIVTGDTKVVEKGKGDSLFVNTAGL
                     GTIDHSMPIGPRHARIGDAILLNGDLGRHGMAIQSVRDGLEFEGPIESDCAPLADIVM
                     GLIEAGIAVHCLRDLTRGGLAAGLNEIAATARIQIDIDESAVPVSEPVRGACEILGFD
                     PLYVANEGRMIAFVPESDADRALAIMRALPVGDGACRIGSVLSDSPGLVTLKSRIGAR
                     RILDLLSGEQLPRIC"
BASE COUNT         1074 a         2068 c         2040 g         1221 t
ORIGIN      
        1 attggagccg agcaggtaga gaagggtgtg cgccatgtcc gcctggaacc cgagcacccg
       61 aggctggccg acgccttcga ggacctgaag catgtacttc caggaatgca tgccgcccca
      121 gcaaatctcc ccttcggccg caagtcgttc cccgcagccc ttggccacct tggcggcctc
      181 tttgaacgtc tcgatgatgc gcttggtgtt gcccttggga tccttgtccc actcggtcac
      241 gctagcggac gaatcgatcc gcacaacgcc gcccgaacgc aagccgagtt ttcggaacgc
      301 gcttgcgatc cggcaccctt tccgcacttg cccgacgaat ttcttccgct cctcctcacc
      361 gcccatggcc gacccgccgc ctgtcccctt ccagaccggg gcgacgaccg acccgatcgc
      421 caagcccttg cccgcgatct tgtccgccac gcgcttcaag tcgtcgtcgg tcgaatcgat
      481 gtcgatgtgg ggagcggaca ggaacaggtc gacgccgtcg aacttgacgc cgttggcctc
      541 ggccttggcg gtcaaatcga gcatggtatc cagatcaatg ccgggctcgg cgccggggcc
      601 gcctttgccg acgaggccgg gccacattgc attgtgcagt ttcgggtagt tgttggcgga
      661 gttgctcatc acagaacctt tcaaagcagg cacttcgaca ctctcatcag gcggcaagaa
      721 gggtctttgg cttgctgtta cgccccggtc gcatcgagtg ctctctggat tttccattga
      781 gcctacccgc gccggcttcg ggcaatcaac gccaaactgc ggcgttggcc ccactcgatc
      841 cacgatccca ggacgatgtg cggcacgaga tgcaggattg cgccagacac cgggctgcgg
      901 cataacctgt cgccacaatg gacattcgca aaatcggatt gacgatgttg ggggtgctgg
      961 ccacgggcgt ctgggcccag caacccatca ctgaagctga aaccaacgcg ctggcggcga
     1021 tccgcgcgca gcgcaccgac gggctgatca ccgtgcagcc gtgggtccaa ctcctcgact
     1081 cgaaccggct cggggtgggc tggatcacgt cgcaaccgtc cgacggcgcg gtggaatgga
     1141 cgcagtcgga gaacggcggc gaatggcggc aggcgtggtt ttcggaggac ggtttgagac
     1201 aggcgaatac cgcgatgcag cgcgcggtga tcggcggctt cgacccggcc aagccgattc
     1261 gctttcgggc cagatcccgg gccatcgagt cgttcaagcc gtacagtgtt gcgttcggcc
     1321 agccaacagt ctcccaggac cgcagcctgc ccgcgcgctc acgtccgaac ggcgctgtct
     1381 cgttcctcgt cttcaacgac atccacaacc gggcgcagtt gtatccgctg ctggtcgaga
     1441 aagcgggaat gccggtcgat ttcgcggtct tcaacgggga tgtgctcaac gatccgcaga
     1501 ccgagtcgga ggtcgtcgac aacctgctga tgccgatggc ctggttcgcc gcgaagtcga
     1561 tcccctgctt ctttctgcgc ggcaatcacg aaacgcgcgg cgcatttgcg cggcggttga
     1621 aggactatct catcctcccc ggggaacgct actacgccgc gatgacgttc ggttcggcgc
     1681 gtgtggtgtt cctcgacacg ggcgaagaca agccggacac gagcaaggaa tacagcggcc
     1741 tggtcgattt cgatccgtac atcgaggcgc agacggactg gctccgacga gagatcgcgg
     1801 gcgacgcctt ccagcaggcg acctggcgcc tggtcgtgat gcacatgccg cccgattggc
     1861 gcatggatga ggccaagctc tggcacggtc agcgtcgcgt caatgagcga ttcgccccgc
     1921 tcctggacgc gggcaaagtc accgcggtga tcagcggtca cacccacgct ccggaggttg
     1981 tcgagccgtg tcctgacgcg agccgcggct tccagtggcc ggtgttcatc ggcggagcac
     2041 cgtctctcgc caaggccacc gtttttagag tggatgcgga cgcaacgacg ctcaagatca
     2101 cgcgctttgc cagcgacggc acggtgggcg cggagcgcac gtggaagaag tgaactcgcg
     2161 tgaaggagag ggcgaaggcg cgggacaaag acggacggaa gacgccggga gccgctcctg
     2221 cggtcgagga cctggatttg acctgagacg cgcctcgccg gaaatgaagt ccggcaacga
     2281 tgtctgcccc aatatcccaa tcgccgacgc aagttgtcag cgtcccacag gaagtcacgg
     2341 gacagccccg tcgcgtggtc gtgggccggg taacgtggcg agacacgttc gccgcgcttc
     2401 ggtatcgcaa ttacagactc tttttcgttg gccaactggt gtcgctgatc gggtcgtgga
     2461 tgcacaacac ggcccaggga tggttggtat accagttgac ggggtcgaaa gtgctgctgg
     2521 gcacggtggc ggctgtcgga tcgctgccca tgctgctgct ctcgatgtgg ggcggttcgg
     2581 tagccgaccg ccatcccaaa cgcgtcgtca tctgctgcac ccaaatcgcc atgatgctcc
     2641 tggccttcgc gttcgcggcg ctggtctggt ccggaaaggt ccagccgtgg cacatcctcg
     2701 ttctggcggc gctggacggg gccgcgatgg ccttcgacat gccggcacga caggccttca
     2761 tggtcgagat caccgcacgc gacgacctga tgaacgcagt gtcgctcaac agttcgatcg
     2821 tcaacggcgc acgcgtcatc ggccccgccg tcgcgggatt cctcatggcc aatgtgggca
     2881 tgacgctctg cttcgtctta aacggaatca gctacttcgc cgtcatcgcc ggcctgctca
     2941 tgatgcgcct gccaaagttc gtccctcctc cgcagccggc ttcggccggc cgccacatcc
     3001 tcgatggctt tgcctacgcg gccgctcatc ggcgtttgcg cgccctgttt ctgctcttag
     3061 gcgttgtcgg cattttcggg tggtcgtact ccgtcctttt gccggcgtac gcgacggaca
     3121 ttctgggcgt gagcgagagc ggatacggtg cgcttttgag cgcaaacggt ctcggggcgc
     3181 tgctgggcgc cctgacggtg gcgaccttcg gcagccggat gcagccccgc ctgctgatcc
     3241 tgggggggct gtggaccttt tccgccatgt tgctgctgct ggcctttgtg cgttggttgc
     3301 cgctcgtcct gctctgcctg gcggtgggcg ggtggggctt gctcctttac ttcgccacca
     3361 cgaacacgct catccagacc agcgtgtccg acgcgatgcg cggacgcgtg atgggcatct
     3421 gggggctggt gttcggcggc atggtgccca tcgggggact cgaatcgggc gtgctctccc
     3481 aggcggtcgg cgtcccctgg acaatcacag tgggagccgc ggtctgcgcc ggtgcgggcc
     3541 tggtcgcatg gtggcagggg cgcagccagc cggggccgtc cctcaatccg cgcccgtgag
     3601 tgctggccaa gacgccgatc atccggctgg catatttgtg cgcagccgca accaaaagtc
     3661 ggagggccga ctccgccatt cgctgaaagg gcctcgtgga actcgaccct ctgtgacccg
     3721 tcgccggccg ggcgaattcc gggggatcgt cgcacgaagg agcgccgatg aacggacttc
     3781 atgccgttcc atggagaatc gctgaaacgc ctatcctcac cttgatgctc gccttcgtgc
     3841 ttttcgtggt gagcttgggc gaggattgaa gttccctcct cttcgcttga cggcacggct
     3901 ccaggtccgc gaccgtaggg gtgccgatga agtttatcga tgagtatcgc gacgccgatc
     3961 gcgcacagga atgcgcgcgc gccattgcac gctcggtgac ccgtccctgg acgatcatgg
     4021 aggtgtgcgg ggggcagacc cacgccatcg tgaagttcgg catcgacgag cttctgcccc
     4081 gggacatcac cctcgtccac ggtcccggct gtcccgtctg cgtcacgccc ctcgagttga
     4141 tcgacaaggc gatcgccatc gcctcgaaac ccggtgtcat cttctgttcg ttcggcgaca
     4201 tgctccgcgt tcccggaagc cacaccgacc tcctgtcggt gaaggcaagc ggcggcgatg
     4261 tgcgcatggt ctactccccc atggactccg tccgcctcgc ccgcgagcat cgggatcgac
     4321 aggtcgtgtt ctttgccgtt ggcttcgaga ccacggctcc cgccaacgcc atggcggcgt
     4381 tccaggcgcg acgggagcaa ctgaccaact tctcgctgct cgtctcgcat gccctggtgc
     4441 cgcccgccat atcgaccgtc ctcggcgccc cccacaaccg tgtccaggct tttctggcgg
     4501 caggccacgt ctgcgccgtc atgggctacc atcagtacga gccattggcc cgggaatacc
     4561 gcgttccgat cgtggtgacc ggcttcgagc cgatcgacat cttgcagggc atccggcgtt
     4621 gcgtgctcca actcgaagcg ggcgaggcca aggtcgagaa ccaatacgcc cgcgccgtga
     4681 aacgggaagg gaaccttccc gcccagcaca tggtccgcga ggtcttccag gtgatcgacc
     4741 gcaagtggcg cgggatcggt gtcatcccgc gcagcggctt cgggctgagc ccggcctatg
     4801 ccgaattcga cgccgaacgt cgattcgggg tcgccgacta ccacgcggat gagccgtccg
     4861 agtgcctgag cgggctcgtg ctccagggcg tcaggaagcc ggttgattgc cctgcgttcg
     4921 gcacccgatg cacaccggag catccgctgg gcgccaccat ggtttccaat gaaggggcct
     4981 gcgccgccta ctatcgctac cgacgccaac aggaaccgca ccgatcttta tgacctctga
     5041 cgtcaccgcc gcttgccccc tgcccatcgg ccagtacacg cacgtcctca tggcccatgg
     5101 aggcggcgga cgcctgatgc aacagttgat cgatcaggtc ttcatctccg cattttcgaa
     5161 tccgcgcctg gacgagcgtc acgacggcgc gatcttggat gcccaaggac gccggttcgc
     5221 attcacaacc gattcgtacg tcgtgcgccc tctgttcttt ccgggtggcg acatcggcac
     5281 gctcgcggtc aacggcacgg tcaatgacct agccatgtgc ggcgcgcgtc cccgctggct
     5341 gagcgccggc ttcatcctcg aagagggtct gccgatcgag accctcgaac gcatcgtggc
     5401 ctcgatgcga caagccgccg caactgcggg cgtcgagatc gtcacgggcg acacgaaagt
     5461 ggtcgagaaa ggcaaaggcg attccctgtt tgtcaacacc gcaggcctgg gcacgatcga
     5521 tcacagtatg ccgattggcc cgcgtcacgc gcggattggc gacgcgatcc tgctcaacgg
     5581 cgacctggga cgacacggga tggcgatcca atccgtgcgc gatgggctcg aattcgaggg
     5641 gccgatcgaa agcgattgcg ccccgctggc ggacatcgtc atggggttga tcgaggccgg
     5701 aatcgcagtg cattgcctgc gcgacctgac ccgcggcggc ctcgccgccg gtttgaacga
     5761 aatcgcggcg accgcccgta tccaaatcga catcgacgaa agcgccgtgc ccgtgagcga
     5821 gccggttcga ggcgcatgcg aaatcctcgg cttcgatccg ctctacgtgg ccaatgaagg
     5881 ccggatgatc gcctttgtgc ccgaatccga tgccgaccgg gcgctggcga tcatgcgcgc
     5941 tttgccggtc ggagacggtg cgtgcaggat cgggtccgtt ctctccgatt cgccgggcct
     6001 ggtgacgctg aaaagccgga ttggcgcccg ccggattctc gatctgctca gcggcgagca
     6061 gttacctcgg atttgttgag gcccaccggc atccgatcgc acaggatgtc gggcgatgct
     6121 cggcgatgac tcgacggctc ggtttcctcc gggtctccgg atgttctgga ccgacggaat
     6181 cgaatagaca cgtccaaccg ggtggccctg tcgccgagtt gcatccgcgt tcgatgcttt
     6241 ggacacgacg cgactcgggc gacgacaacc ggttcagcat ggaatccgtg ccaaatcagt
     6301 caagttcttg cgcctcgcag cgggggtggt tggcacggtt atttcatcta aattcctgaa
     6361 gggttgctgt tgttgaggtt ggaaatagcg cttatcaatt ggc
//
LOCUS       DAOQRO010000186         6374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12166_length_6374_cov_5.809305, whole genome shotgun sequence.
ACCESSION   DAOQRO010000186 DAOQRO010000000
VERSION     DAOQRO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12166_length_6374_cov_5.809305"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKK20_08035"
     CDS             <1..324
                     /locus_tag="PKK20_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99877.1"
                     /translation="YFIQALHTEGGGGDYLKVAWRIEGDETPAGQLTPISGAVISAYA
                     PVAAPEFDPPVITGTGQITLSWSGAGALQQSPDFKTWTPVAGNPTSPYTVAPSAAQMF
                     YRIAR"
     gene            complement(422..2062)
                     /locus_tag="PKK20_08040"
     CDS             complement(422..2062)
                     /locus_tag="PKK20_08040"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNU99878.1"
                     /translation="MSNPAITSPAPPPRRIREVARDLGLDPEEILPHGHYIAKIPVAE
                     LDARANQPDGRLILVSAMTPTPQGEGKTTATIGLADALRRLGHRAMFCLREPSLGPYF
                     GIKGGGTGGGRAQVIPAEDINLHFVGDMYSVEKANNLLCAMLDNHLQHGNELGIDPRR
                     IVMRRVIDFNERSLRDIVIGLGGPLNGVPRRDGFNITPASEVMAILCLARDYRDLGER
                     MARIVVGFTYKGAPIRADAVQAPGAMQVLLRDAIHPNLVQSLEGTPAFVHGGPFANIA
                     QGTNTSIATRLALKLADYVVTEAGFATDLGAEKFFHIKCRAAGLTPATAVIVATVKAI
                     AYHGGFKEIGGLGNLAKHIENIRLFGLDPVVCVSHFPDDNPNDYARIVRFCARQGVEA
                     VVSKHFDQGGQGAIDLARAVVRAATRRRRRALRFLYPLDAPIRKKLEVIATKVYGADG
                     VNFDRAALNHIETLDQHGFNTLPVCVAKTPLSLSDRADLRGRPGGFSITVNELRLSAG
                     AGFVVAVCGNIVTMPGLPKVPAAARIRMLPSGQATGLT"
     gene            complement(2237..4648)
                     /locus_tag="PKK20_08045"
     CDS             complement(2237..4648)
                     /locus_tag="PKK20_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNU99879.1"
                     /translation="MSLIRLPFFLMLVCLLRIGSTARADEEQDQIAILQSAANLAQKS
                     AACQRLRVIGTAKSIPVLASLLTQEGTSHFARHALEALPFPEAGAALRAALARTSDKP
                     QLQAGIIDSIGWRREPESIEPLRPLLASTNLDLAITAASALGRIGGGAVALLRSANDQ
                     SHGAPVSMVFRSVVQESLLQCAETLHGADGTRLASEICRSVLSQPASESIRVVAWRGL
                     VLRDARSAPDLVRTALSGRDRSLQRAALGLVRELDDASILVACLGDWDDLPAESQAAL
                     LDANVRRGKAPLPLVRKALDSSNLALRVAALDALADLADPSSIPSVAKSAARGEASER
                     EAAARTLTRLRGPGVREALLAAIDTADPVEKAVLLRALGARADRQAGDTLLRFAGAEA
                     QPVRLAALESLRQLALPETLAPLLDLAAKAKSDDQIDPVFKALVAVCQSSPNTTETEG
                     RVIAAIRNSAPAERRLLLPLLAELGTADALIAARSATQDSNSELVRTAVRVLGQWPNP
                     EPALYLTDFAQFATDLGLHALALRGAVEVSAHEQDTAKRVALLEKAMSVARRADEKRL
                     ALAQMAQISSADALETALKNLAKPDLAEEAGLAAIAIAEKIASADSALADAAAANVLA
                     RCKAAETVRRAWALRRTPAINGPFIRHWLVSGPYRQAGVEGATAVFELTFAPEKADAK
                     VDWKPTPVADQVDLSSLFPGHANCVAYLRAEIVAEQDSDALLLMGSDDGLKVWLNDAV
                     VHSNNVDRGLIVDQDRAPIRLRKGANRLLLKVTQGGGGWAACARIAGIDGQRVPGLRI
                     EPVQP"
     gene            complement(4748..6136)
                     /locus_tag="PKK20_08050"
     CDS             complement(4748..6136)
                     /locus_tag="PKK20_08050"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99880.1"
                     /translation="MQSIQPYRMSTRRQFLKIAATGAVALTAPSVIPASILGRAGQVP
                     PSERIGMGFIGLGGQGTGHLLGGAWTYVPGGYAARTDVQVLAVCDVRRERREQAQERC
                     NQMYAQRLGQAAYDGVEAYNDFREVLARPDIDALLLALPYHWAAPMATMALRAGKDVY
                     CEKPIAITVREGQRLVETCRHFGRVYQAGTQQRSEYGGKFRLACELVRNGRIGQLKEV
                     YAYRQPGALFPSAWTSEHAVPVPPGFDWDMWLGPLPWRPFGGEAGHTLSGLFVGDVNW
                     SPHHYDIVQWTVNPDPTAPIEVQYEPSREKPGTSAHPGGAAGRPEDGQVHYRYANGVV
                     VHSTPYPGENIGHDGGACFVGTQGRIAVDRGAIVSYPASILKEPLRPDDARVYFTDSH
                     SGNFLECVRTRRPTICNPEVATYTMNAILIGGIAMGLERSLKWDPGKHEFVGDEQANR
                     LLSYAPRPPWTV"
BASE COUNT         1091 a         2021 c         2175 g         1087 t
ORIGIN      
        1 tacttcattc aggccctgca caccgagggt ggcggcggcg actatctcaa ggtcgcatgg
       61 cggatcgagg gcgatgagac ccccgcaggg caattgaccc cgatctcggg cgcggtgatc
      121 tccgcctacg cgccggtggc ggcgccggaa ttcgatccgc cggtgatcac cgggaccggt
      181 cagatcacgc tctcgtggtc gggagcgggc gcgctgcagc aatcgccgga cttcaagacc
      241 tggacgcccg tggcgggcaa tccgaccagt ccgtacacgg tcgctccaag cgccgcgcag
      301 atgttctatc gcatcgcgcg gtagcccgag agacgattcg acacgagaga ccgggggcaa
      361 ccccggtctc tctttttgtt catcaggctc gctggcgtcg gggcccatag cccctacaca
      421 atcacgtcag cccggtggcc tgacctgagg ggagcatccg gattcgcgcc gccgcaggca
      481 ctttgggcag gccgggcatg gtgacgatgt tgccgcagac cgcgacgaca aagccggcgc
      541 ccgccgagag ccgaagctcg ttcaccgtga tggagaagcc gccgggccgg ccgcgcaggt
      601 cggcgcggtc cgagagcgag agtggcgtct tggcgacgca cacgggtagc gtgttgaagc
      661 cgtgttgatc gagagtctcg atgtggttca acgcggcgcg gtcgaaattg acgccgtcgg
      721 cgccgtagac cttggtggcg atgacctcga gtttcttgcg gatcggcgca tccagagggt
      781 agagaaagcg cagtgcccgg cgtctgcgcc gtgtcgccgc ccggaccacg gcccgcgcga
      841 ggtcgatcgc tccctggccg ccctggtcga agtgtttcga caccaccgcc tcgactccct
      901 gacgagcgca aaaacggacg atccgagcgt agtcgttagg attgtcatcc gggaaatgac
      961 tgacgcagac cacggggtcg agtccgaaca accggatgtt ctcgatgtgt ttggccaggt
     1021 tgcccagacc gccgatctcc ttgaagccgc cgtggtacgc gatcgctttg accgtggcga
     1081 caatgaccgc cgtggccggg gtcagccccg ccgcccggca tttgatgtga aagaacttct
     1141 ccgctccaag gtcggtggca aagccggcct cggtgacgac gtaatcggcg agcttgagcg
     1201 ccaggcgggt tgcgatgctg gtgttggtgc cttgggcgat gttggcgaag gggccgccgt
     1261 gcacgaatgc cggcgtgcct tcgagcgatt gcaccaggtt gggatgaatg gcgtcccgca
     1321 gcagcacctg catggcgccg ggggcctgca cggcgtcggc ccggatgggc gcccccttgt
     1381 acgtgaaccc cacgacaatg cgcgccatcc gctccccaag atcgcgataa tcccgggcga
     1441 ggcagagaat ggccatgacc tcggaggcgg gcgtgatgtt gaagccgtcg cgacgcggca
     1501 ctccgttgag cggtccgccc aggccgatca cgatgtcccg cagcgagcgt tcgttgaagt
     1561 cgatgacgcg gcgcattacg atgcggcgcg gatcgatgcc cagttcgttg ccatgctgga
     1621 ggtggttgtc caacatggcg cacaggaggt tgttggcctt ctcgacggaa tacatgtcgc
     1681 cgacgaaatg cagattgatg tcctccgccg ggatgacctg cgcgcgcccg ccgccggtgc
     1741 cgccgccttt gatgccgaaa tagggaccca gggacggctc acgcagacaa aacatggcgc
     1801 ggtgacccag gcggcgcaga gcatcggcca gaccgatcgt cgcggtggtc ttgccttcgc
     1861 cctgcggcgt cggcgtcatc gccgacacca ggattaggcg gccatcgggc tggttggctc
     1921 gagcatcgag ttccgcgacc ggaatcttgg cgatgtaatg gccgtgcggg agaatctcct
     1981 cggggtcaag acccaggtcg cgggcgactt cgcggatgcg tcggggcggg ggggctggac
     2041 tggtgatggc tggattggac atagaatccc agaacgacgg aacagggtgc gctcatgtcg
     2101 agtctggccc tcaaccctat ccccttcttt tcgcagggat tctaccgagc gaggcgcggg
     2161 atagagggac gggggaagaa cggggactct tcatggaaat gcggactggg tttggtttga
     2221 ggccggcctc tggcggtcag ggctgtacag gctcgattcg cagtcccgga acgcgctggc
     2281 catcgattcc ggcaatccgg gcgcacgcgg cccagccgcc gccgccctgg gtgactttca
     2341 gcagcagtcg attcgcgcct ttcctgagtc ggattggcgc gcggtcctga tcgacgatca
     2401 ggcctcgatc cacgttgttg ctgtgcacaa ccgcgtcgtt cagccagacc ttgagaccgt
     2461 cgtcgctgcc catgagcagc agggcgtcgg agtcctgctc cgccacaatc tcggcgcgca
     2521 ggtaggcgac gcaattggcg tggcccggga aaagactcga gagatcgacc tgatcggcga
     2581 ctggcgtggg cttccagtca accttcgcgt ccgctttctc cggtgcgaac gtcagttcga
     2641 aaacagcggt cgctccctcg actccggctt gtcgatacgg cccgctcacc agccagtgcc
     2701 ggatgaaagg tccgttgatt gccggcgtcc ggcgcagggc ccaggcgcgc cggacggtct
     2761 ccgccgcctt gcaccgggcc agcacgttcg cggcggctgc gtccgccaac gccgaatcgg
     2821 cggacgcaat cttctcggca atcgcaatag cggccagccc ggcttcctcg gccagatccg
     2881 gtttggccag gttcttcaag gcggtctcga gggcgtccgc cgagctgatc tgcgccatct
     2941 gagcaagagc caggcgcttc tcgtccgccc gtcgcgcgac gctcatcgcc ttctcgagca
     3001 acgcaacgcg tttggccgtg tcctgctcgt gcgcgctgac ctcgacagcg ccgcgcaacg
     3061 cgagcgcgtg caaccccaag tcggtggcga attgcgcgaa atcggtcaga taaagcgcgg
     3121 gctctggatt gggccattga cccaaaacgc gcaccgcggt tcggacgagt tcggagttcg
     3181 agtcctgcgt ggcgctccgc gcggcaatca aggcgtccgc ggtccccagt tcagcgagca
     3241 gaggcaacag cagtcggcgc tcggccggtg cggagttccg gatggcggcg atgacgcgtc
     3301 cttctgtttc ggttgtgttc gggctcgatt gacaaacggc cacaagggcc ttgaacaccg
     3361 gatcgatctg atcgtcggac ttcgccttgg cggcaagatc gagcaggggc gccagcgtct
     3421 cgggcagggc aagctgcctg agcgattcga gcgcggccag acgcacaggc tgcgcctcgg
     3481 cccctgcgaa tcgaagcagc gtatcgccgg cctggcggtc cgcacgcgcg cccagcgccc
     3541 gcagcagaac ggccttctcg accgggtccg ccgtgtcgat cgccgccaga agcgcctcgc
     3601 gcacgcccgg accgcgaagg cgcgtcaggg tccgagcggc ggcctcgcgc tcggacgctt
     3661 cgcctcgagc cgcacttttc gccacggacg gaatcgacga tggatcggcc aggtcggcca
     3721 gggcatcgag ggcggcgact ctcagcgcga gattcgagct gtccagggcc ttgcggacga
     3781 gcgggagagg ggccttgccg cgccggacgt tggcatcgag cagcgcagcc tgggactcgg
     3841 cgggcagatc atcccaatcc cccaggcagg cgaccaggat cgaggcatcg tccagctcgc
     3901 gcaccagacc gagcgcggcg cgctggagcg aacggtcgcg tccggacaaa gccgtgcgga
     3961 cgaggtcggg cgcggatcgg gcatcgcgca acacgaggcc gcgccacgcg acaacgcgta
     4021 tcgactcgga agcgggctga gacaacacgc tgcggcatat ctcggacgcg agcctggtcc
     4081 cgtcggcgcc gtgcagcgtc tcggcgcatt ggagcaggct ttcctggaca accgaccgaa
     4141 agaccatcga gacgggcgcg ccgtggctct ggtcgtttgc gctgcggaga agggcgacgg
     4201 cgccgccgcc gatgcgtccg agggccgaag cggcggttat ggcgagatcg aggttagtcg
     4261 aagccagcaa tggccgcagc ggttcaatcg actcgggttc gcggcgccaa ccgatcgagt
     4321 cgatgatgcc ggcctggagt tgcggcttgt ccgaggtcct tgccagggcg gcgcgaagag
     4381 cagcgcccgc ttccgggaac ggcaaggctt cgagggcgtg ccgcgcgaag tgcgaggtcc
     4441 cttcctgtgt caggagagag gccagcaccg ggatcgactt ggctgtgcca atcacccgaa
     4501 gcctctggca cgcggccgat ttctgcgcga ggttggcggc cgattggagg atggcgatct
     4561 gatcctgttc ctcgtccgcg cgcgcagtcg agccgatgcg cagcagacag acgagcatga
     4621 ggaagaaggg gaggcgaatc aggctcatag ggtctgtgca aaaagcggtt tatgcaagcc
     4681 ctcgcggatg tctggacatg gtgtctcttg gttccatcgc cgtctcggat gtcggaaccc
     4741 catcgagtca caccgtccag ggcggacggg gcgcatagga gaggaggcgg ttggcctgct
     4801 cgtcgccgac aaactcgtgc ttgccggggt cccacttgag cgagcgttcg aggcccatcg
     4861 cgattccgcc gatgaggatc gcgttcattg tgtaggtggc gacttcggga ttgcaaatcg
     4921 tcggacgccg ggtgcgcacg cattcgagga aattgcccga atggctgtcg gtgaaataga
     4981 cgcgcgcatc gtcagggcgc agcggttcct tgaggatgct ggcggggtac gaaacgatcg
     5041 ctccccgatc gacggcaatg cggccttggg ttcccacaaa acaagcgccg ccatcgtggc
     5101 cgatattctc cccgggataa ggtgtcgagt gcaccacgac gccgttggcg tagcgataat
     5161 gcacctggcc atcctcgggc cggcccgcgg ctccgcctgg gtgcgcggac gtccctggct
     5221 tttcccggct cggctcgtac tggacttcga tcggcgcggt tgggtccgga ttcacggtcc
     5281 actggacgat gtcgtagtgg tgtggactcc agttcacatc gccgacgaac aggccggaga
     5341 gcgtatggcc tgcttcgccc ccaaacggac gccacggcag cggtccgagc cacatgtccc
     5401 agtcgaaacc cggtggcacg ggcaccgcat gctcggaggt ccaggccgac gggaacaggg
     5461 cgccgggctg gcgataggca tagacttcct tgagttggcc gatgcgtccg ttgcggacca
     5521 gttcacaggc gagtcggaac ttgccgccgt actcggaacg ctgctgggtg ccggcctggt
     5581 aaacgcgtcc gaaatgccgg caggtttcga cgagtctttg accctcgcgg acggtgatgg
     5641 cgatcggctt ttcgcaatag acatccttgc ctgcccgaag ggccatggtg gccatcgggg
     5701 cggcccaatg atacggcaaa gccagcaaca acgcatcaat gtcgggtcgg gcgaggacct
     5761 cgcggaaatc gttgtaagct tcgacaccgt cgtaggcggc ctggcccagc cgttgcgcgt
     5821 acatctggtt acatcgctct tgggcctgct cgcggcgttc ccgccgcaca tcgcacactg
     5881 cgagcacctg gacgtccgtg cgggccgcgt agccgcccgg cacataggtc caggcaccgc
     5941 ccagcagatg ccctgtcccc tggccgccca ggccgatgaa acccatcccg atgcgctcgc
     6001 tgggggggac ctggccggcg cggcccagga tcgaagccgg aatcacagag ggcgccgtca
     6061 gcgcaacagc ccctgttgcg gcgatcttga ggaattggcg acgcgtcgac atgcgatacg
     6121 gctggatgct ttgcatgatc ttgtctctgt atgtcgatcg atccgttatc ggttgtcttt
     6181 cgcacgccaa atgcggcaag aaagacctct ataaacagtg tccatgccac gaaagtcacg
     6241 cacgaaattg gccggtgcca actcgccaac tcgcctcgaa cacattcttt aaccccgttc
     6301 gtaggggacg aggccctgcc tgccctgcct gccctgcctg ccctgcctgc cggcaggcag
     6361 gcggcaggca ggcg
//
LOCUS       DAOQRO010000187         6369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12185_length_6369_cov_4.843522, whole genome shotgun sequence.
ACCESSION   DAOQRO010000187 DAOQRO010000000
VERSION     DAOQRO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12185_length_6369_cov_4.843522"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKK20_08055"
     CDS             complement(<1..915)
                     /locus_tag="PKK20_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNU99881.1"
                     /translation="MKTLPFEDDVKRRRTFAIISHPDAGKTTLTEKLLLYGGAVQLAG
                     SVTARKHQRATTSDWMELEKKRGISVSSTVLQFEFGGYCVNLLDTPGHKDFSEDTYRV
                     LTAVDAAVMVIDAGKGIESQTRKLFEVCRQRGIPIFTFMNKLDRPARGPLMLLDELER
                     VLGLHPCPLNWPLGDGADFRGVYDRRSLQVHFFERVPGGAFRAPVSVHDLGDSAVRDA
                     LPADVYTRVVEELAMLEGAGSRFDPARILAGEQTPVCFGSAVHNFGVQLLLDAFLELA
                     PTPQPRVANGHVVSPAGDGFSGFIFKMQA"
     gene            1270..2667
                     /locus_tag="PKK20_08060"
     CDS             1270..2667
                     /locus_tag="PKK20_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99882.1"
                     /translation="MHIRFALVLVLACAWSVRLPAADSPIAAQSIAPTLCLATFDIDA
                     TPPIGSQMAYDPVTNRWDLGLRVRGIVLSGAGDPIVLCAVDWIGIANGGHDAFREALA
                     RAASTSVRRVAVHSLHQHDAPDCDFSAERILKEAGLDARQYEGTFQREVIANLERAVA
                     ASLAQAQPVTHLGLGEARVDQVASNRRLLGEDGKVRATRWTTTLDPALRAEPEGTIDP
                     VVSLISFWNGERPLAVLSYYATHPQSYYRTGLPNPDFPGLARFLRQLAVPSALHVHFN
                     GAGGNLGAGKYNDGAHENRLRLAERLAAGMRQAWETTERRPIRPGDVQWTVESVALPP
                     AKHLSASALEAAVRGDQGRPPASSAASRLAWVRRCESGQHPDVACLALGRARLLHLPG
                     ELFVEYQLAAKAMRPDLFVAMAAYGDYAPWYIGTAVAYEQGGYETSPEASNVAPDAEA
                     VLLSAIRKLLDEPIP"
     gene            complement(2833..4425)
                     /locus_tag="PKK20_08065"
     CDS             complement(2833..4425)
                     /locus_tag="PKK20_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99883.1"
                     /translation="MLCAGSVTQAAAPAGSKERLRRLVRLPRLSLTLGMSFDPLRGIV
                     LMPDRVLAMYEIEDLRKGMQDNASDAPRHTRIGRLLDGIGSHNEARQAYTNAVSLYRR
                     QGAEQSDDGPTLADYGAALHGLGELDAAERVLRRGVQVAPAAWKPHAALGRLLSAQAL
                     ASLVPATGSAGADPFKALLSGEAASGKLKPAQLEQAQTRIEESVLCYDRAIESAPDVA
                     EPYTGRAAVRVFHNLLGWLRNPDAESEPQPVRLARAMYPPEALPDLRQAMRLAPKDPM
                     ALGTALLFELFSSALEQGSLQSESIGTGNSWANLPERTRQPVRESLTRLEEMSQDTES
                     HTAAAALELLGSLQCFVVNDVPGAEQTLRRATALDSKRTQAWETLIFILATSKRSEAL
                     QAVCEDRLKAEESARARLFLAKSHEMRSDWNRVFETTETLERRYPEDVYANLAAAAAI
                     LHTTRDEAGLRRAAQSIAKAEKLLGAAPARDEVVNLLFLRGLLFALADATEPALHQLR
                     QIKQIDPHFEGIEEALEIVEQM"
     gene            4568..5374
                     /locus_tag="PKK20_08070"
     CDS             4568..5374
                     /locus_tag="PKK20_08070"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNU99884.1"
                     /translation="MSLVTQSMTQPLTIAGRSFGSRLLVGTGKFASPEAMRDALAASG
                     TGIVTVALRRADLSGRKDAFANILEYVDPARYLVLPNTSGAMNASEAVRLARLAAAAG
                     LPKWVKLEIHPDPRYLLPDPVETLAAAQMLVQEGFTVLPYINADPVLAKRLEEAGTAT
                     VMPLGSPIGSNRGLQTRDQLRIIIEQATVPVVVDAGLGAPSHAAEAMELGADAVLVNT
                     AIAVASDPSRMAVAFKQAVEAGRAAFEMGLAAEQQTASPTSPLTAFLGAA"
     gene            5371..>6369
                     /gene="rfaE1"
                     /locus_tag="PKK20_08075"
     CDS             5371..>6369
                     /gene="rfaE1"
                     /locus_tag="PKK20_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNU99885.1"
                     /translation="MNESIPTLSVSRVRALLAAAACRRILVVGDVMLDQFVWGEVSRI
                     SPEAPVPVIDFERESFMPGGAANVARNLTALGVQTELFSVVGRDDPGARLRRLLEENH
                     VACDGCLSHATRPTSVKTRILAHRQQVVRVDRETRNDLDADATDALLDAMRARLDGAD
                     AVIVGDYGKGVVSQTLLDQLRVFCRERGIWLSLDPKPVHSLDLSRLSLITPNRKEAFE
                     LAGEPDGARSGGKGLDTALMRTVDRLLSKLQPALLLVTLGEQGMLLCQRARPPVHIPT
                     VTRDVFDVSGAGDTVIATFTLAIAAGGSPVEAAVLSNHAAGVVVGKVGTATVQGGEL"
BASE COUNT         1062 a         2026 c         2162 g         1119 t
ORIGIN      
        1 cgcctgcatc ttgaagatga accccgagaa accgtccccc gcgggcgaca ccacgtgtcc
       61 gtttgccacg cgcggctggg gggtcggcgc gagctcgaga aatgcgtcca gcaaaagctg
      121 caccccgaaa ttgtgcaccg cgctgccaaa gcacaccggc gtctgttccc ctgccagaat
      181 tcgcgccggg tcgaagcgcg atccggcgcc ttcgagcatt gccagttcct cgacgacgcg
      241 cgtgtagaca tcggcgggca gcgcgtcccg cacggcggaa tccccgagat catgcaccga
      301 gaccggggcg cggaaggcgc cgcccggcac gcgctcgaaa aaatgcactt gcagcgaacg
      361 gcggtcatag acgccgcgaa agtcggcgcc atcgcccagg ggccagttga gcggacacgg
      421 gtgcaggccg aggacccgtt cgagttcgtc cagcagcatc aaaggacccc gcgccgggcg
      481 atcgagtttg ttcatgaagg tgaaaatggg aatcccgcgc tggcggcaga cctcgaagag
      541 cttgcgtgtc tggctctcga tgcccttgcc cgcatcgatc accatcaccg ccgcgtccac
      601 cgccgtcaga acgcggtagg tgtcctccga gaagtccttg tgaccgggcg tatcgagcag
      661 gttgacgcag tagccgccga actcgaactg cagcaccgtc gaactgacgg agattccgcg
      721 cttcttctcg agttccatcc aatccgacgt ggtggcgcgc tggtgcttgc gcgccgtgac
      781 cgatccggcc aactgcacgg cgccgccgta gagcagcagc ttctcggtca gcgtcgtctt
      841 gcccgcgtcc gggtgcgata tgatcgcaaa cgtgcggcgg cgtttcacat cgtcttcaaa
      901 aggcaacgtc ttcatggctt ggttcgttcc cgaacatctt cgatcggtcg aggtcgttgt
      961 tcccgtcaga atccggcgca aatgcctgcg tggaacatcc gtcgcgcgga ccgcggaggc
     1021 cgggactctg ggcgcggaac gcgtcggatc agacgagaac ggccccacca gcccggcagg
     1081 cgggacggag acggtcaggg tggagtcaga gtgtggaggt tcgccgcgcc acgggccggg
     1141 cgaacgccga ttggatcgct caatgctgtc accgtcaaga ttctcacggt catcactgta
     1201 gcacgactgg caggcccggc ggcaagactt tctcttttcg cggactgcaa cccggcttaa
     1261 catgccccca tgcatatccg cttcgctctt gtccttgttt tggcgtgcgc gtggagcgtt
     1321 cgccttccgg ccgcagactc gccgatcgcc gcgcagtcaa tcgcgccaac gctgtgcctg
     1381 gctacgttcg acatcgatgc gacgccgccc atcggatccc aaatggccta cgatccggtg
     1441 accaaccgct gggacctcgg gttgcgggtg cgcggaatcg tgctgtcggg ggcgggcgac
     1501 ccgatcgtgc tctgcgcggt ggactggatc gggatcgcca acggaggcca tgacgcgttt
     1561 cgtgaggcgc ttgcccgggc ggccagcaca tcggtccgcc gcgtggccgt gcattcgctc
     1621 caccagcacg acgccccgga ttgcgacttc tcggcggagc gcattctcaa ggaggccggg
     1681 ctcgatgcac gccagtacga gggcaccttt cagcgcgagg tgattgcgaa tctggaacga
     1741 gccgtggctg cgtcgctggc tcaggcacag cccgtcaccc atctggggct gggcgaggcg
     1801 cgcgtcgacc aggtcgcgtc aaaccggcgc cttctgggcg aggacggcaa ggtgcgggcg
     1861 acacgctgga ccaccaccct cgatcccgca ctgcgcgccg agcccgaggg cacgattgac
     1921 cctgtcgtgt ccctgatcag cttctggaac ggggaacgac cgctggccgt tctgagctac
     1981 tacgcaaccc acccgcaaag ctactaccgc accggcttgc cgaatccgga cttccccggc
     2041 ctggcccggt ttctccgcca actggcggtg ccgtccgcct tgcatgtcca tttcaacggt
     2101 gcgggcggca atctgggcgc aggcaagtac aacgacggcg cgcatgagaa taggctgcgt
     2161 ctggccgagc ggcttgcggc cggcatgcgc caggcctggg agacaaccga gcgccggccc
     2221 atcaggccag gcgacgtgca gtggacggtc gagtcggtgg cgctgccgcc tgcgaaacat
     2281 ctgagtgcgt ccgcactcga agccgctgtc cgcggcgatc aaggcagacc cccggcgtcg
     2341 agtgcggcgt cccggctcgc ctgggtgcgc cggtgcgagt ccggacaaca cccagatgtg
     2401 gcctgcctgg cgctgggtag ggcccgtttg ctgcatcttc caggcgagct gtttgtcgaa
     2461 taccagcttg ccgccaaggc gatgcgtccg gatctattcg tggccatggc tgcgtatgga
     2521 gactacgccc catggtatat tggcaccgcc gtggcttacg aacagggtgg atacgagacc
     2581 agccccgagg cctcgaatgt cgcgccggac gcggaggccg tcctgctgag cgcgatccga
     2641 aagctgctcg acgagccgat cccataagcc agattgaggc gatgagccga tcgggcgccg
     2701 aattcatcat gggcgagatt gggcgtgtcc cggacgcgct ggtctgcctg cccatgggct
     2761 cgtcaccggc acgggcatac gaactgctat cggtgaagct caggagtgtg ggtcgatgcg
     2821 accgcacggc ctctacatct gttcgacgat ttccagcgct tcctcgatcc cctcgaaatg
     2881 cggatcgatc tgtttgatct gcctgagttg gtggagggcg ggttcggtgg cgtcggccag
     2941 ggcgaagagg aggccgcgca ggaagagcag gttgacgact tcgtcgcggg cgggcgcggc
     3001 gccgagcagt ttctcggctt tggcgatgga ctgggcggcg cggcgcaggc cggcctcgtc
     3061 ccgtgtggtg tggaggatgg cggcggcggc ggcgagattg gcgtacacgt cctccgggta
     3121 gcgccgttcg agggtctcgg tcgtttcgaa gacccggttc caatccgacc gcatctcgtg
     3181 gctcttggcc aggaacaggc gtgcgcgggc ggattcctcg gctttcaacc ggtcctcgca
     3241 gaccgcctga agcgcctcag accgtttgga cgtggcgagg ataaagatga gcgtctccca
     3301 ggcctgggtt cgtttcgaat ccaacgctgt ggcgcgacgc agggtttgct cggcccccgg
     3361 cacatcattg acgacaaaac actgcaagga ccccagcaat tcgagcgcgg cagccgcggt
     3421 gtggctttcc gtgtcctggc tcatttcttc gagtcgggtg agcgattcgc ggaccggttg
     3481 gcgggttcgc tcgggcagat tcgcccagga attgccggtg ccgatggatt cggactgcaa
     3541 cgatccctgt tcgagcgcgc tgctgaacaa ctcgaacaac agggctgtgc ccagggccat
     3601 cgggtccttc ggcgcaagcc gcattgcctg ccgcaggtcc ggcagcgcct ccggcgggta
     3661 catcgccctg gccaaacgca cgggctgcgg ttccgactcg gcatcggggt tgcgcaacca
     3721 tccgagaaga ttgtgaaaga cccggaccgc ggcgcgtccg gtatacggct ctgcaacgtc
     3781 gggcgccgac tcgatcgcgc ggtcgtagca gagaacagac tcctcgattc gcgtttgggc
     3841 ctgttcgagt tgggcgggct tgagctttcc ggatgcggcc tcaccggaga gcaaagcctt
     3901 gaacggatca gctcctgcgc ttcccgtcgc cggcacgagc gatgcaagcg cttgggcgga
     3961 cagcaatctg cccagggcag cgtgcggttt ccatgcggcg ggcgccacct ggacgccgcg
     4021 gcgcagcacc cgctccgcgg catccagttc cccgagaccg tgcaacgcgg cgccgtagtc
     4081 ggccagcgtc ggtccgtcat cgctttgctc ggcgccctga cggcgataca gagaaacggc
     4141 attggtgtag gcctggcggg cctcgttgtg actgccgatc ccatcgagca gccgcccgat
     4201 ccgcgtgtgt cgcggagcgt cgctggcgtt gtcctgcatc cccttgcgca aatcctcgat
     4261 ctcatacatg gccaggacac ggtcgggcat gagaacgatg ccgcgcaatg gatcgaagct
     4321 catgccgagg gtcagactga ggcgaggcag gcgtacaaga cgccgcaggc gttctttgct
     4381 gcccgcgggc gcggcggcct gtgtcacgga accggcgcag agcatgagtg caagggcaat
     4441 gaagatgggc gcgcgcattt tgctgcgcga caatacacgc tgacagcaca ggtgacaaat
     4501 ccgggcgctt gtctggtcgc cggtttgcgc ctcgacacac gtcggagaac gcgtctagtt
     4561 taccggcatg tcgctggtca ctcagtcgat gacccaaccc ttgacgattg cggggcgctc
     4621 gttcggatcg cgcctgctcg tcggcacggg caagttcgca tcgccggaag cgatgcgtga
     4681 tgccttggcg gcgagcggaa ccgggatcgt gaccgtggcg ttgcggcgcg cggatttgag
     4741 tgggcggaag gatgcttttg ccaacatcct cgaatatgtg gatccggcgc gctacctggt
     4801 tttgccgaac acgagcggcg cgatgaacgc aagcgaggcg gttcggctgg cgcggctggc
     4861 ggcggcggca ggtttgccca agtgggtcaa actcgaaatc cacccggacc cgcgttatct
     4921 gctgcccgat ccggtcgaga ccctcgcggc cgcccagatg cttgtgcagg agggcttcac
     4981 ggtgcttccg tacatcaatg cggatccggt gttggcgaaa cggctcgagg aagccggcac
     5041 ggccaccgtc atgccgctgg gatcgcccat cgggtccaat cgcggtctcc agacgcgaga
     5101 tcaattgcgc atcatcatcg agcaggcgac ggtcccggtc gtggtggacg cgggcttggg
     5161 ggcgcccagc cacgccgcgg aggcgatgga actgggagcg gacgccgtgc tggtgaacac
     5221 ggcgatcgcg gtggcgagcg atcccagccg catggccgtc gcgttcaagc aggcggttga
     5281 agcgggacga gcggccttcg agatgggttt ggccgccgag cagcagaccg ccagtccgac
     5341 aagcccgctc accgccttcc tcggagcggc atgaacgagt ccattccgac tctcagcgtc
     5401 tcgcgcgtac gcgccctgct ggcggccgcg gcctgccggc ggattctcgt tgtcggcgac
     5461 gtgatgctcg atcagtttgt ctggggcgag gtgagccgaa tctcgccgga ggcccccgtc
     5521 cccgtgattg atttcgaacg ggagagcttc atgcccggcg gagccgcgaa tgtcgcccgt
     5581 aacctcacgg ccctcggagt ccaaaccgag ttgttcagcg ttgtgggacg cgacgatccg
     5641 ggcgcacggt tgcgtcggct cctcgaagag aaccacgtcg cctgcgatgg atgcctgagc
     5701 catgcgaccc ggccgacgag cgtcaagacc cgcattctgg cgcatcggca gcaggtcgtg
     5761 cgggtcgatc gcgagacgcg caacgatctc gatgcggacg ccacggacgc cttgctggac
     5821 gcgatgcggg cgcgcctgga cggggccgat gccgtcatcg ttggcgatta cggcaaaggg
     5881 gtcgtgtccc agaccctcct ggaccaactg agggtcttct gtcgggaacg aggcatctgg
     5941 ctgagccttg atcccaaacc ggtccacagc ttggatttga gccggctctc gctcatcacg
     6001 cccaatcgga aggaggcctt cgagttggcg ggcgaacccg acggtgccag aagcggcggc
     6061 aagggtttgg acacggctct tatgcgcacc gtcgatcgac ttctgtcgaa actgcagccg
     6121 gccctcctgc ttgtgacatt gggcgagcag ggcatgctgt tgtgccagcg cgcacgaccc
     6181 ccggttcaca tcccgacagt aacccgcgat gtgtttgacg tgtcgggggc gggcgacacc
     6241 gtgatcgcca cgttcacgtt ggcgatcgcc gccggaggct cgccggtcga ggccgccgtc
     6301 ctgtcgaacc atgccgcggg cgtggttgtt ggaaaggttg ggacagcgac cgttcagggc
     6361 ggggaattg
//
LOCUS       DAOQRO010000188         6361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12202_length_6361_cov_3.913416, whole genome shotgun sequence.
ACCESSION   DAOQRO010000188 DAOQRO010000000
VERSION     DAOQRO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12202_length_6361_cov_3.913416"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..3838
                     /locus_tag="PKK20_08080"
     CDS             80..3838
                     /locus_tag="PKK20_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNU99886.1"
                     /translation="MKTVRGSRLLTDRPGAQQSSGARLRPAQRDVKARSHSNPQRQAR
                     PPRAGARSATEADERLVAAAGALPRLDAAPAPIPLVVRSHYSFLNSTLSPGAIVDLAR
                     AGGFPAVALCDQGNLHGAVEFAMAAKAAGVKAIIGVEIRCDAAPLWLYVENATGYGNL
                     CRLISDKAQSEAAARKGRAEPAGRTPGLDFAARELAAAPRDGLLAVSTDAGLARLFPG
                     RFYRAIRTRGELRRHKFDPGGLPVVPVFPVHYARADCRWRFDVVQSIRTLTLLRQAHP
                     AKVLDSKWSFRAADELAALFGRYPELAAATRELADRCDFVFRFGQPQFPDFRPPDGST
                     PRQFLRHLVLRGLEARYGAGRAGRLMGQVEEELAMIGTVGYEEYFLVVWDLLRSCRER
                     GIDWITRGSAADSLVCYCLGISGVCPIRFDLYFRRFLNAERMAMHKLPDIDIDFPHDR
                     KDDVVDLLFAKYGRDHCAVVGGFSTFRARSAFAEVAKVFGVSERDVRRFTEHFPWSIG
                     EGWPGSGSPDGARAREAGGRLESLLRASPECRDLPLDEEPFASALAMAEFLDGVPRHP
                     KMHPCGVVLSRQPMSELTATFVSQKGYPTTQFDMDAVEAIGLVKMDILAQGGLAVMRD
                     VKGMLRERGIATDCERFTLNHSSGEARCDAPAPRASLEAGPVRRADPVVAGEALIDEV
                     KRIESGTGGDRPSEQSNTDQMPSSERSCTADISLLFEARHGASAFDDPEVWELIAGGH
                     ARGVHHIESPAMTSLCQMCNVRDIDTLIAIVSVIRPGAANEDKKREFARRYQGLSAVA
                     YPHPAIADCLRSTFGLVVYEEQILQICEAFAGLPGGRADMLRRGLVKDDARVVAEIAP
                     EFAEAARRLGRTEDAIRRVWELVIGFHGYAFCKAHSTAYGVEAYQSAWLKRSFPAEYM
                     AGVLSNGKGFYRPLVYVLECHRLGIPLVGPWVNDPGPGFRVIERPEPLPGAGERPSMP
                     RSASGRAIRVPVGSIESLSARTREAIVAERARGAYASLSDFYLRVQPRLEEMEVLSRA
                     GAFDGFGRSRCEQFWEMQHAHRRWGGEGTVGQPWLLPLVARDMPSTAVAVRGGAPAES
                     GAGGAALDLTEPSRVQRLRWEVELLGFPASGHPLELYPDVAWETYCPVARLGRFIGQE
                     ITLCGLVIEDRVHHQATGEPMKFLTLADWTGMIETELFARTYKTYGLATVRYPVLEVT
                     ARVEPFENGRGHSLRVVRAGRPRLVNRQSRVDRSANACCPDGGVRTPP"
     gene            complement(3765..4148)
                     /locus_tag="PKK20_08085"
     CDS             complement(3765..4148)
                     /locus_tag="PKK20_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99887.1"
                     /translation="MLSNARYLWLPALVLLLVGCARNQITNMSPRVMRRTPAGLYTLE
                     ARWDTNQRAVRDDTMTPFVVVGTQFHPMRRSTLNDRCWRATVPVPPEQRFLNYRFKFD
                     YLKAGFGRRHPDSKRSRFYQLEIVD"
     gene            4237..5301
                     /locus_tag="PKK20_08090"
     CDS             4237..5301
                     /locus_tag="PKK20_08090"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99888.1"
                     /translation="MNNGIAVAGLCWLLAQAEVGMAAEKVFDLTGVKPNEAPPGFRSA
                     LSGTGQPGEWKVILDDAPLTFAPMTREAPQTRKIPVIAQLSRDRTDERFPLLVYDEEV
                     FGDFKLRVRFKTVDGVVEQMAGVAFRLQDERNFYVVRASTLGRTFRFYRVHQGIRDTS
                     IGPEIAIPSGVWHELSIECQGNRIRLRLDGEQPIPEITDPTFANGKIALWTKSDSVSY
                     FTDLRIEYTPREPLAKALVRDALEKYPRLLGLRIVSTTSRRPELHVVASSNPGEEGQP
                     GNAYEKQCIEQNIVLFGKSDRKAIVTMPLHDRNGDAIAAVRCELETFFGQTGQNAVAR
                     AVPIRKRMEPRVTSLKELTE"
     gene            complement(5395..6336)
                     /locus_tag="PKK20_08095"
     CDS             complement(5395..6336)
                     /locus_tag="PKK20_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99889.1"
                     /translation="MNSRVRCAMVFLLAAGTLLGQVTVETVFEHEQYLANERVRVGVR
                     ITNFSGQTLRLGSSPDWLDLMVETESGELVSQRSDPPIVEPFEVPSSSRATRWVDLIP
                     HYDVGHVGLYKVTPTVRLAELGQTVIGKPASVQVTGGARIWEQDFGVRLAEGPAAGAL
                     EVRRYALIQAMNQKTVTLYVRVSDQQEAQVFNVIPIGPVLAFSRPEAQVDHSGLLHVL
                     SQTSARQFTYCVISPSGELTVRQAHRYTNSRPALRASEDGSVIVRGGIRTISPSDLPQ
                     SAKESVEPIAPPPTAPVEKKPDATGKNTPKKKPKPKK"
BASE COUNT         1017 a         1782 c         2352 g         1210 t
ORIGIN      
        1 gtctgacatc cgggctctgg caaaaccgcg cccagaaagg gaaccgggag cattctgtcg
       61 gtcctgtggg tggtctgcca tgaaaacagt gcgtggcagc cggctgttga cggaccggcc
      121 tggggcgcag cagagttcgg gggccaggct gcgacctgcg cagcgtgatg tgaaggcgcg
      181 ttcccactcg aatccgcaac gccaggcccg cccgccacgg gcaggagctc gttcggcgac
      241 ggaagcagac gagcgtctcg tcgccgcggc gggggctctg ccgcgcctcg atgccgcgcc
      301 ggcgccgatc ccgctggtgg tgcggagtca ctactcgttc ttgaactcga cgctatctcc
      361 cggggcaatc gtcgatttgg cgcgcgccgg cggatttccc gcggtggcgc tttgtgacca
      421 gggcaatctg cacggcgccg tcgagttcgc catggcggcc aaggcggcgg gggtaaaggc
      481 gatcatcggg gtggaaatcc gatgcgatgc cgctccgctt tggctctacg tcgagaacgc
      541 gacggggtat gggaatctgt gccggttgat ttccgacaag gcccagagtg aggcggccgc
      601 gcgcaagggg agggcggagc cggcagggcg tacgccggga ctggatttcg cggcgcgcga
      661 gctggcggcg gcgccgaggg acggtcttct ggccgtctcg accgatgccg ggctcgcccg
      721 attgtttccg ggtcgtttct accgtgcgat tcggacgcgc ggggagttgc gccggcacaa
      781 gttcgatccc ggcgggttgc cggtcgtgcc ggtctttccg gtccactatg ccagggcgga
      841 ctgtcgatgg cggtttgacg tggtccagag cattcggacg ctgacgttgc tgcgtcaggc
      901 gcatccggcg aaggtgctcg atagcaaatg gtctttccgc gccgcggacg aactggccgc
      961 tctgtttggg cggtatccgg aactggcggc ggcgacgcgg gagttggctg atcgctgcga
     1021 ttttgtcttt cgattcggcc agccgcagtt tcccgacttt cgcccgcccg acggttcgac
     1081 cccgcgccag tttctgcggc acttggtatt gcggggattg gaggcgcggt atggagcggg
     1141 gcgggcgggg cggctgatgg ggcaggtcga ggaggagttg gcgatgatcg gcacggtggg
     1201 ctatgaggag tacttcctgg ttgtgtggga tttgctgagg tcgtgtcggg agcggggaat
     1261 tgactggatc acgcggggca gcgcggccga ttccctggtc tgctactgtc tgggcatcag
     1321 cggggtctgt ccgatccgat tcgatctcta ttttcggcgt ttcctgaacg ccgagcgcat
     1381 ggcgatgcac aagctccccg acatcgacat cgatttcccc catgaccgca aggacgacgt
     1441 ggtcgatttg ctttttgcca agtacggtcg ggaccattgt gcggtcgtgg gcggattttc
     1501 gacatttcgg gcgcgcagcg cgtttgcgga ggtggcgaag gtgttcggtg tttcggagcg
     1561 cgacgtgcgt cgtttcaccg agcatttccc ctggagcata ggcgagggat ggcctgggag
     1621 cgggagtccg gacggggcgc gggcgaggga ggcgggcgga cgtctcgagt cgttgttgcg
     1681 ggccagcccg gagtgccgcg acctgccgtt ggacgaagag ccatttgcca gcgcgttggc
     1741 gatggcggag ttcctcgatg gggtgccacg gcacccgaag atgcatccgt gcggcgtggt
     1801 gctgtcacga cagccgatga gcgaactcac cgccacattc gtttcgcaga aaggctatcc
     1861 gacgacgcaa ttcgacatgg acgccgtcga ggcgatcggc ttggtgaaga tggacattct
     1921 ggcccagggg gggttggcgg tgatgcggga tgtgaaggga atgttgcggg aacgcgggat
     1981 agcgacggat tgcgaacgat tcacgctcaa ccactcgtca ggggaggcgc gttgcgatgc
     2041 gcccgctccg cgcgcttccc ttgaagcagg ccctgttcgg agggccgatc ccgtggttgc
     2101 aggagaggct ctgattgacg aagtcaagcg tatcgagtca gggactggcg gagatcgtcc
     2161 ttccgagcaa tccaacacgg accaaatgcc gtcctccgag cgctcctgca ctgcggatat
     2221 ttcgcttctt tttgaggctc ggcatggcgc gagcgcgttc gacgatccgg aggtgtggga
     2281 gttgattgcg ggaggtcacg cccggggtgt gcatcacatc gagtctccgg ccatgacgtc
     2341 gctgtgtcag atgtgcaacg tgcgcgacat cgacacgctg attgcgattg tgagcgtgat
     2401 ccgtccgggg gcggccaacg aagacaagaa acgcgagttc gcgcggcgct accaggggct
     2461 gagcgcggtg gcgtatccgc atccggcgat tgcggactgt ttgcgcagca cgttcggcct
     2521 ggtggtttac gaagagcaaa tattgcagat atgcgaggcg tttgccgggc tgcccggggg
     2581 gcgggcggac atgctgcggc gcgggctggt gaaggacgat gcgagggtgg ttgccgagat
     2641 cgcgcctgag tttgccgagg cagcgcggcg tttggggcgg acggaggacg caatcaggcg
     2701 ggtgtgggag ttggtgatcg gttttcacgg gtacgcgttt tgcaaggcgc acagcacggc
     2761 ttacggagtc gaggcctatc agagcgcatg gctgaagcgg tcttttccgg cggagtacat
     2821 ggcgggcgtg ttgagcaacg gaaaggggtt ctatcgcccc ctggtgtacg tgctcgagtg
     2881 tcatcggctc ggcattccgt tggtcggtcc ctgggtgaac gatcctgggc cgggcttccg
     2941 tgtgattgag aggcccgagc cgttgccggg agccggcgag cgcccgtcaa tgccccggag
     3001 cgcgtcggga cgcgccatcc gtgtgccggt tggctcgatc gagtcgctca gcgcgcggac
     3061 ccgcgaggcc attgtcgccg agcgcgcccg gggcgcgtat gcgtcgctgt cggatttcta
     3121 tctgcgggtc cagccccggc tcgaggagat ggaggtgttg tcgagggcgg gggcttttga
     3181 cggtttcggg cggagccgtt gcgagcagtt ttgggagatg cagcatgcgc accggcgctg
     3241 gggcggggaa gggactgtcg ggcagccgtg gctgctgccg ctcgtcgcgc gggatatgcc
     3301 gtcgaccgcc gtcgcggttc gaggcggcgc gcccgcggag tcgggggcgg ggggggcggc
     3361 tcttgacctg accgagccga gtcgtgtgca acggttgcgg tgggaggtcg agttgctggg
     3421 gtttccggcc agtgggcatc cgctcgaatt gtaccccgac gttgcgtggg agacgtactg
     3481 cccggtggcg cgtctggggc ggttcatcgg acaggagatc acgctgtgcg gtctggtgat
     3541 cgaggatcgg gtgcaccacc aggccacggg cgagccgatg aagttcctga cgctggcgga
     3601 ctggacgggg atgatcgaga cggaactctt tgcgcgcacg tacaagacat acggcctggc
     3661 cacggtgcgg tatccggtgt tggaggtgac agcgcgggtc gaaccgtttg agaacggtcg
     3721 gggccattcg ctgcgtgtcg tgcgggccgg gcggccgcgt ttggtcaatc gacaatctcg
     3781 agttgataga agcgcgaacg cttgctgtcc ggatggcggc gtccgaaccc cgccttgaga
     3841 tagtcgaact tgaatcggta gttgaggaag cgctgctccg gtggcacggg cacggtggcc
     3901 ctccagcacc ggtcattgag agtcgagcgc cgcatggggt ggaattgggt gccgacaacc
     3961 acgaagggcg tcatggtgtc gtcgcgcacg gcgcgctggt tggtatccca gcgcgcttcg
     4021 agcgtgtaga ggccggccgg cgtccgccgc atcacccgtg gcgacatgtt ggtgatctgg
     4081 tttcgggcgc agccgaccag gagcaggacc agggcaggca gccagaggta tcgcgcgttg
     4141 ctcagcatgt ccgaaccgtt actggagggg cgcgtggaag taaaggcgga acctgcgcgc
     4201 ttgagccgcg tcggacgtct gctattttca gcgcccatga acaacgggat cgcagtggcg
     4261 ggactgtgct ggctgctcgc gcaggccgag gtggggatgg ccgcggaaaa agtctttgac
     4321 ctgacgggcg tgaaaccgaa tgaggcgccg ccgggatttc gaagcgccct ttcgggaacg
     4381 gggcagccgg gcgagtggaa ggtcatcctc gatgacgccc cgctcacgtt tgcgccgatg
     4441 acgcgcgagg caccccagac ccgcaagatt cctgtcatcg cccagctttc gcgggaccga
     4501 accgacgagc ggtttccgct gttggtttat gacgaggagg tttttggcga tttcaagctg
     4561 agggtgcggt tcaagaccgt cgatggggtt gtcgaacaga tggccggggt ggcctttcgg
     4621 ctccaggacg aacgcaactt ctacgttgtg cgcgccagca cgctggggcg caccttccgc
     4681 ttctaccggg ttcatcaggg catccgagac acttcgatcg gcccggagat tgccatcccg
     4741 agcggcgttt ggcacgagct gtcgatcgag tgtcagggca atcggattcg actgcgtctt
     4801 gacggggagc aaccgattcc ggagatcacg gacccgacgt ttgcgaacgg caagatcgct
     4861 ctgtggacga agtcggactc ggtgagttac ttcaccgatc tgcggatcga gtacacgccg
     4921 cgcgagccgc tggccaaggc gcttgtgcgg gacgcgctgg agaagtaccc gcgtctgttg
     4981 ggtctgcgga ttgtgtcgac cacgagccgg cggcccgagt tgcatgtggt ggccagcagc
     5041 aatcccggcg aggaaggcca gcccggcaac gcgtatgaga agcaatgcat cgaacagaac
     5101 atcgttctgt tcggcaagag cgatcggaaa gccattgtga cgatgcccct ccacgaccgc
     5161 aacggcgacg ccatcgccgc cgtgcggtgt gaactcgaga ccttttttgg ccagaccggg
     5221 cagaatgccg tcgcgcgggc cgttccgatc cgcaagcgga tggagccccg cgtgacgtcc
     5281 ctcaaggagt tgaccgaatg aggcggcgat ggccttggcg ttgcgcggcc ggcgacgaat
     5341 cttggaaggc cgaggaccgt ctttcgtggg ttcccgttta gagggcagga gaaatcactt
     5401 cttgggcttg ggcttcttct tgggagtgtt tttgccggtc gcatcaggct tcttctcgac
     5461 aggcgcggtt ggtggcgggg cgatgggttc gacagattcc tttgccgatt gcgggaggtc
     5521 tgagggcgag atggtgcgga tgccgcctcg gacgatgaca ctgccgtctt ccgatgcgcg
     5581 caaggccggg cggctgttcg tgtagcggtg ggcctggcgg acggtcagtt cgccgctggg
     5641 cgagatgacg cagtaggtga actgccgcgc gctggtttga gagaggacgt ggagcaggcc
     5701 ggagtggtcc acctgcgcct cggggcggct gaaggcaagg accgggccga tcggaatgac
     5761 attgaagacc tgcgcctcct gttggtcgga gacgcggaca tagagtgtga ccgttttctg
     5821 attcatggcc tggatcaggg cgtagcggcg cacctccaat gcgccggccg cggggccttc
     5881 agcgaggcgg acgccgaagt cctgctccca gatccgggct ccgccggtga cctggacgct
     5941 ggccggcttg ccgatcacgg tctggcccag ttccgcaaga cgcacggtcg gagtgacttt
     6001 gtacaggccg acgtgtccca cgtcgtagtg gggaatcagg tcgacccatc gagtcgctct
     6061 ggacgaggac ggcacctcga atggctcgac gatcggcggg tctgagcgct gcgagaccag
     6121 ctcaccgctc tcggtttcca ccatcagatc gagccagtcc gggctcgatc ccaaccggag
     6181 cgtctggccg gagaaattgg tgatgcgcac cccgacccgc acgcgttcgt tggcgaggta
     6241 ttgctcgtgt tcaaagaccg tctcgacggt cacctggccc aacagcgttc cggccgccag
     6301 caggaagacc atggcacatc gcacccgact gttcatggaa gcaatctacg ggccgcgcgc
     6361 g
//
LOCUS       DAOQRO010000189         6353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12229_length_6353_cov_4.402826, whole genome shotgun sequence.
ACCESSION   DAOQRO010000189 DAOQRO010000000
VERSION     DAOQRO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12229_length_6353_cov_4.402826"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKK20_08100"
     CDS             complement(<1..187)
                     /locus_tag="PKK20_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNU99890.1"
                     /translation="MEEPVHDFGRVRNGEVVKHTYVFSNVGNQVLDVTDVRASCGCTT
                     MGGWTRQVKPGETGNIPI"
     gene            complement(740..2395)
                     /gene="hcp"
                     /locus_tag="PKK20_08105"
                     /gene_synonym="priS"
     CDS             complement(740..2395)
                     /gene="hcp"
                     /locus_tag="PKK20_08105"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNU99891.1"
                     /translation="MFCYQCEQTSKGTGCTVHSTCGKDETTAALQDLLLHAAKGVAQY
                     NHRARQLGVSDREIDRYIVKALFITVTNVNFDADAVERAVRAGATMRDRARALYERAA
                     TAAGKTPEVLKGPAIWEPAPTQSGLVTQAGTASILVRRDQFGADVVGLQELLIYGLKG
                     AAAYADHAVVLGMESDEVYAFFSEALTHVVETPPSVDTLFAMNMKCGEVNLKVMEMLD
                     RAHTSTFGHPVPTPVRIEPVKGKCILVSGHDLHDLEVLLKQTEGKGVNIYTHGEMLPA
                     HGYPGLKKYRHLVGNFGGAWQDQKKEFAQFPGAILMTTNCIIEPQNSYKGRIFSTGNV
                     GWPGVRYVPNGDFSPVIDAALAAPGFAEDGPDKTILVGFGHNAVLGVADQVVAAVKSG
                     AIRHFFLIGGCDGAKTGRNYYTDFAELAPKDTVILTLACGKFRFNKLDFGTIGGIPRL
                     LDAGQCNDAYSAIQIALALSKAFDCGVNELPLSLILSWFEQKAVAILLTLLYLGIRNI
                     RIGPSMPAFITPAVLQVLVDKFNLKPIAARAQDDLDAILGRAA"
     gene            complement(2697..2900)
                     /locus_tag="PKK20_08110"
     CDS             complement(2697..2900)
                     /locus_tag="PKK20_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99892.1"
                     /translation="MPKGYERGKTPKQPNHMVDWLNCIRTRSTPKCSFDEAFIETATF
                     LMSLTAQQEQRMVRWDAVREEIV"
     gene            complement(3142..4020)
                     /locus_tag="PKK20_08115"
     CDS             complement(3142..4020)
                     /locus_tag="PKK20_08115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNU99893.1"
                     /translation="MREGPVGTRWSLTTTLIAINTGVFVLGLIAALIWPQGYWRLFGM
                     LALSPTGLARGWLWQILTFQFLHGSLFHLIINCVVLYFFGRPLEESLGRPFFLRLYFG
                     SGAVGGILQVALAWVFPGHFGMSGVVGASAGVFGLIAAFALLNRETPITTLIAFVIPV
                     TMRAKYLLVFEALIALLGMLSPGTGVAHAAHLGGMLGAIGYLQLRQLAARSHLRRLRL
                     RPTVIHRELVGATAPRVRAQRRRQAMDAGPSSDADFMSREVDPILDKISTQGIHSLTE
                     REKKILETARAKMSSQ"
     gene            4376..4996
                     /locus_tag="PKK20_08120"
     CDS             4376..4996
                     /locus_tag="PKK20_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNU99894.1"
                     /translation="MQRNSRWNRRWQSLVFALFLIQTASALAADALALEWEERFRRLN
                     GMVEELLGAQASLQKRIGTLSDEVRVARDEQGRSDTRFVRHDDLRKALDALADKIREL
                     DRKREEDKKLILDELRKLAQNPLPETPSRRVRRTEPEPTPAPATVLKGREHIVKSGET
                     LAAILAAYQQDGVRVTQAQVVKANPGLHPDRLSVGQKLFIPDPSAP"
     gene            5370..5446
                     /locus_tag="PKK20_08125"
     tRNA            5370..5446
                     /locus_tag="PKK20_08125"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5404..5406,aa:Pro,seq:tgg)
     gene            complement(5586..>6353)
                     /locus_tag="PKK20_08130"
     CDS             complement(5586..>6353)
                     /locus_tag="PKK20_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNU99895.1"
                     /translation="SGDHEHTAGMLLLAKCLAGHPGVLTACYLNGWPKDSTAYDNADA
                     IVIYSDGGGGHPFIQGNRLEILGEKMRNGAGLGLLHYAVEVPREKAGKQFLEWAGGYF
                     EVHWSVNPFWHPQFNNLPDHPVTRGVKPFGVLDEWYYHMRFPEGMKGVTPILSALPPA
                     DTLNRPDGSHSGNPEVRKAVLERKEPQHVMWVFDRPDGGRGFGFTGGHSHKAWGNDDF
                     RKVVLNAIVWIAHAEVPPDGVQSTVTSDDLKLNLDSK"
BASE COUNT         1220 a         2053 c         1903 g         1177 t
ORIGIN      
        1 gaatcggtat gttcccggtc tcgcctggct tcacttgccg agtccatccg cccatcgtcg
       61 tgcacccgca agaagcccgc acatccgtga catccaacac ctggttgccg acgttcgaga
      121 acacgtatgt gtgcttcacc acctcgccgt tccgcaccct gccgaagtcg tggacaggct
      181 cttccacctt caggcgcggt ccgtccccat gaaccggttg ccccccggaa gactgcgtgg
      241 ccgcaactgg attttgatga gccgttccca cggaaaccgg cgcatagaca cgtggagggg
      301 gggatggagg cgcgggggga ctttggcgtt gctgctgtgc atggcaaaca gaccccgcca
      361 acagcagaat ggcacccagt acaaacgcgc gcgcctgcac acgtctgacc gggacagagg
      421 ggtctgtctg ttgtgatgcg gaagactcga tggggctgtt ctcagggact cgagccacga
      481 gatacgattc gatgtgattt ctagtcatgt acctatgtca gccggggctc cgcgcgcata
      541 tccgcgcgtc tcaattgaca ctcgcacgaa catggcggtg aaccacttcc tcgtcaagcg
      601 aaccgattcc tctcgaacgc gcggcttgcc cgacaagcaa aacgcctgtc tgccggccag
      661 cagaggctgg cgccagacag gcgctcgaaa cgcttgcgcg ggagcgaacc tcgccacgcg
      721 cgcggcggcg acgctcgact caggccgcgc ggcccagaat agcgtccaga tcgtcttgcg
      781 ccctcgcggc aatcggcttc aggttgaact tgtccacaag aacctgcagg acggcgggcg
      841 tgatgaacgc cggcatggaa ggtccaatgc gaatattgcg gatgccgaga tagagcagag
      901 tcaggaggat cgcaacggcc ttttgctcga accacgagag aatgagcgag agggggagtt
      961 cgttgacgcc gcaatcgaac gccttcgaca aggccaaggc aatctgaatg gccgagtaag
     1021 cgtcgttgca ctgaccggca tcgagcagcc gcggaatgcc tccaatcgtc ccaaaatcga
     1081 gcttgttgaa tcggaacttg ccgcacgcca gggtgaggat gaccgtgtcc ttgggggcca
     1141 attcggcaaa atccgtgtag taattgcgac cggtcttcgc gccgtcgcag ccgccgatca
     1201 ggaagaagtg gcggatggcg ccggacttga cggccgcaac cacctggtcc gcgactccca
     1261 gcacggcgtt gtggccgaag ccgaccagaa tggtcttgtc ggggccgtcc tcggcgaatc
     1321 caggggctgc cagggcggcg tcgatgacgg gagagaagtc gccgttaggg acgtaccgga
     1381 cgcccggcca gccgacgttt ccggtgctga agatgcggcc tttgtagctg ttctgcggct
     1441 cgatgatgca attggtcgtc atgaggatcg cgccggggaa ctgggcgaac tcctttttct
     1501 ggtcctgcca cgccccgccg aagttgccga ccaggtgcct gtatttcttg agtccggggt
     1561 acccgtgcgc gggaagcatc tcaccatgag tgtagatgtt cacgcccttg ccttcggtct
     1621 gtttgagcag cacctcgagg tcgtgcagat cgtggccgga gaccagaatg catttgcctt
     1681 tgaccggctc gatgcggacc ggggtgggca ccgggtgacc gaaagtgctc gtgtgggcgc
     1741 ggtcgagcat ttccatcacc ttcaggttca cctcgccgca cttcatgttc atggcaaaga
     1801 gcgtgtcgac cgaggggggc gtctcgacca catgggtcaa cgcctcgctg aaaaacgcgt
     1861 agacctcgtc tgattccatc cccagcacca cggcatgatc cgcataagcg gcggcgccct
     1921 tgagcccgta gatgagcagt tcctgcaggc caaccacatc ggcgccaaat tgatcgcgac
     1981 gcaccaaaat cgaggccgta cccgcctgag tgacaagccc agactgcgtc ggagcgggct
     2041 cccagatggc gggacctttc aaaacctccg gggtcttgcc cgccgccgtc gccgcccgct
     2101 cgtacaaagc ccgggcgcgg tcgcgcatcg ttgcacccgc gcgcaccgcg cgctcgacgg
     2161 cgtcggcgtc gaaattgaca ttggtgacgg tgatgaagag cgccttcaca atgtaccggt
     2221 cgatctcgcg atcggacacg cccaattgtc gggcgcggtg attgtactgg gccacgcctt
     2281 tggccgcatg gagcaacaag tcttgcagcg cggcggtggt ttcgtccttg ccgcacgtcg
     2341 agtgcacggt acagccggtt cccttcgagg tctgttcaca ctgataacaa aacatgttct
     2401 tggctcctgg ttgttgttct tcggttcttc agcggccgtc gtgggtcact gacccttgcc
     2461 gcaagtttca tatagaacaa catgcccgct atccagaatg caagaattga cctgggtcaa
     2521 tgcgctcctc gatctggaga gccgcgcctg agtccaaagc cagtcatccc cttccataag
     2581 gagtccccgt gtctctccgc ccttctctcc cgcgcctcgt tgcagagaat ccgtgacccc
     2641 cttaacgcag caccgaaact ggacttgtcg acaacatgtt tcaccggtca aggattctac
     2701 acgatttctt cccgcaccgc atcccagcgc accatgcgtt gctcctgctg cgccgtcagt
     2761 gacatcagaa aggtggcggt ctcgataaac gcctcgtcga acgagcactt gggcgtgctc
     2821 cgggtgcgga tgcagttgag ccagtccacc atgtggttgg gttgcttggg agttttcccc
     2881 cgctcgtagc ctttgggcaa atcggctttc cggttgtggc tcgatcagtt tcaacaggta
     2941 gacgatctga tgatcgcccc agtaaccgat gtgagaccac ggatcggacg tatccagaac
     3001 ctcccagtcg atgccgtcgc gcccgatgcg ataggggttg tgaccgtccg cggtcgattg
     3061 cgtagttctc tttcggcttg gggccgagcc cggcagcggg ccggatcgac atccgctgga
     3121 aagacccgca tgccgcgggc gctactggga gctcattttt gcccgagccg tttcgagaat
     3181 cttcttctca cgctcggtga ggctgtggat gccctgcgtg gaaatcttgt ccaggatcgg
     3241 atcgacctcg cgactcatga agtccgcgtc ggaggacggc ccggcatcca tggcctgacg
     3301 ccggcgttgg gctcgaacgc gcggcgcggt cgcgcccacc agttcacgat gaattacggt
     3361 gggacgcaga cgcagccgcc ggaggtggct gcgcgcggcc agttggcgca gttggaggta
     3421 gccgatcgcg cccagcatgc cgcccagatg cgccgcgtgg gccacgcccg tgccgggact
     3481 gagcatgccg agcagcgcaa tcagggcctc gaacacgagc aggtacttcg cgcgcatcgt
     3541 gacagggatc acgaaggcga tcagggtcgt gatgggcgtc tcccgattga gcagcgcgaa
     3601 tgccgcaatg agcccgaaca ctccggccga cgcacccaca acaccgctca tcccgaagtg
     3661 gccggggaag acccaggcaa gagccacttg cagaatgccg ccgacagccc cgctgccgaa
     3721 gtacaaacgc aggaagaacg ggcggccaag gctctcctcc agaggccgtc cgaagaaata
     3781 gagcacgacg caattgatga tcaggtgaaa cagactcccg tgcagaaact ggaaggtgag
     3841 aatctgccag agccagccgc gcgccagccc ggtcggcgac agagccagca tgccgaagag
     3901 ccgccagtac ccctgcggcc aaataagcgc agcgatcaac cccaggacaa acacgcccgt
     3961 gttgattgcg atcagggtgg tcgtcagcga ccaccgcgtg ccgacagggc cttcgcgcat
     4021 atatgcccgg tcctcaatca cagcaaacag actaggcgag cccgcatccc ttttcaatgc
     4081 actttttcgc gctttttcac cctcctgtgc atccgtaagt tgtcggcggg aatcgagttt
     4141 gcgccgcgtg gggcctgcct gccgcgcctg cctgtcccgt gcccgacacg gcagacaggt
     4201 tgctgcgctt tgcggcgcag gcagggcacg cggcttacac cgggagtctc tatccgttgt
     4261 aagcccggtg ccctcaccgg gcgacacaat acggacaaca acccgatgcc ctgttcgccc
     4321 ccgcgcgcgc cgattccatc ttgcaccatc ggcaacccgt ttttatgttg catccatgca
     4381 acggaattcg cgatggaacc ggcggtggca gagcctggtg tttgccctgt tcctcatcca
     4441 gaccgcttcg gccctggccg ccgatgcgct ggccctcgag tgggaggaac gcttccgcag
     4501 actcaatggc atggtcgagg aattgctcgg agcgcaagcc tccctccaga agcggatcgg
     4561 caccctctcc gatgaggtcc gcgtcgcacg ggacgaacag ggccgctccg acacgcgttt
     4621 cgtccgtcac gacgacctga ggaaggcgct cgatgccctc gccgataaaa tccgcgaact
     4681 cgatcggaaa cgggaggagg acaagaagct catcctcgac gaactgcgca agctggccca
     4741 aaaccctctg ccagagacac cgagccgacg ggtcagaagg accgagcccg agccgacacc
     4801 ggcgcctgcc accgtgctca aaggccgcga gcacattgtc aaatcgggcg agactctggc
     4861 cgccattctg gcggcctacc agcaggacgg tgtgcgcgtc acccaagccc aggtggtcaa
     4921 agcaaacccc ggcctccacc cggaccggct cagcgtcgga cagaagctct tcattcccga
     4981 cccgtccgcg ccatgaggcg ttccgacggt ccggcccccc tagccctata cggatttctc
     5041 accactcgaa ggagtcatct gaaatccagg ctgatgtgtg gactgccgca gtgaatctgg
     5101 aggttgtcgg tggaaatcga gtcggcgccg cgtggggcct gcctgccgcg ccagcctgtg
     5161 ccgtgcccga tacggcagac aggccacctc gccttgcggc gcaggcaggc aggcgtcgcc
     5221 ctccggcaat ctccatccgt tgtaggcccg gtgctctcat cgggcgacag aacaccgaca
     5281 acagccagat gcagcgtgac tgctgtgcgc cggtgaattg tcattgcgct tctcgccaat
     5341 ccgcgccagt ctctcgtcca cacttgcgtc ggagcgtagc tcagcctggc agagcacttg
     5401 gtttgggacc aagacgtcgc aggttcaaat cctgtcgctc cgaccatttc ttgcctgtgt
     5461 ccccgcgatc ctctcctctc ctgtccggag ggccgatccc tggtcctcag acaacagccc
     5521 ctcggagaaa acagtccccg gaagaccact gacacgcatc gaaccgactg gcaactcgcc
     5581 acgtgtcact tcgaatcgag attcagcttc agatcatcgg atgtgaccgt cgactggacg
     5641 ccgtcgggcg ggacctcggc atgtgcgatc cagacgatgg cgttgagcac caccttccga
     5701 aaatcgtcgt tcccccacgc cttgtggctg tgaccgcccg tgaaaccgaa tccgcgtccg
     5761 ccatccggac gatcgaacac ccacatgaca tgctgcggtt ccttacgttc gagcacggct
     5821 tttcggacct cggggttgcc gctgtgcgag ccgtccggac ggttcaatgt atccgcgggc
     5881 ggcagcgcgc ttaaaatggg cgtcacgcct ttcatccctt ccgggaaccg catgtgataa
     5941 taccactcgt ccaacacgcc gaacggtttc acgccgcggg tcaccgggtg gtcgggcagg
     6001 ttgttgaatt ggggatgcca gaacgggttc actgaccagt gcacctcgaa atacccgcca
     6061 gcccattcaa ggaactgctt gcctgccttc tccctgggca cctcgaccgc atagtgcagc
     6121 aagccgagtc cagcgccgtt ccgcatcttc tcgccgagaa tctcgagacg attgccctgg
     6181 atgaacggat gcccgccgcc gccatcggag tagatcacga tcgcatccgc attgtcgtac
     6241 gccgtcgagt ctttgggcca cccgtttaaa tagcaggccg tcagaacgcc cggatgaccg
     6301 gccaggcatt tggccaacag cagcatgccc gccgtgtgtt cgtgatcgcc gct
//
LOCUS       DAOQRO010000190         6352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12231_length_6352_cov_5.203589, whole genome shotgun sequence.
ACCESSION   DAOQRO010000190 DAOQRO010000000
VERSION     DAOQRO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12231_length_6352_cov_5.203589"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..945)
                     /locus_tag="PKK20_08135"
     CDS             complement(7..945)
                     /locus_tag="PKK20_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNU99896.1"
                     /translation="MSKGYSEDTLVEQPAIELLAGLGWQSIGAFDEVFGTGGTLGRES
                     AGEVVLLRHLVPALKKLNPQAPPEAIDAAVNDLTRDRSVMSLAAANREVYALLKNGVK
                     VEIRNPEGELVPERIRVIDWDNPANNHFLLVSQLWVTGEMYKRRPDLVGFVNGLPLVV
                     SEFKAVTERVEAAFKDNITDYKTTIPQLFWSNAFILVSNGVESRLGSLTAEWEHYCDW
                     KKISSEAEPGSVSLETLLKGTCDKTRLLDLVENFTLFEEKAGKIRKVVAKNHQYLGVN
                     NAINALLATLTPGPSPSGRGVWSLDIFLLDSKRPLG"
     gene            complement(958..2415)
                     /locus_tag="PKK20_08140"
     CDS             complement(958..2415)
                     /locus_tag="PKK20_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99897.1"
                     /translation="MKAWMTKALDLLRASLEPPKHELNECDWKAGLSPDKKRLTEHLS
                     AFANQPGGGYLVFGVDPTGTASGVDEKTVETTVNQLANLGRQGLEPPVALDHAVEDYG
                     SVRLLFVHVPESAVKPVHLRGKGLEEAFIRSGGTTRKASRQEIGTLMLNSRTPRWEEL
                     HASVLLPDGVFAQRLDIEPIHKMLERPVPTTAEETLAWMESERFIVREPAGGGYVTNL
                     GAIAAARRLADYPDLSRKAVRVVVYEGLNKTKTKQEREGTKGYAISFQGLLQFVMSLL
                     PQSEVIEQALRRKRTVYPEIALRELIANALIHQDFSITGAGPLIEIFDDRIEVSNPGG
                     LLPSKRLDRLIGTQPESRNEQLARAFRRYKICEERGSGLLKAGIQVELYGLPPIEFTA
                     GSNYFKVTLFTPRTFAQMSVRERLEACYQHAVLKYYSDSAMTNKTLRERLKMSEKQRS
                     MVSVLIQEAIDKNLVKAADPSNKSKKFAEYVPFWA"
     gene            complement(2557..3846)
                     /locus_tag="PKK20_08145"
     CDS             complement(2557..3846)
                     /locus_tag="PKK20_08145"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNU99898.1"
                     /translation="MAARAKWPTKLLGDLAEFRNGVNYDKRSFGQGIKVVGVKDFQNY
                     TKPLYAELEQINPEGIVTERNVLQKGDIVFVRSNGNRELIGRSLYIDDLQEQVTHSAF
                     TIRLRFTSPEAVSRFYAYVFRTPIIRKGLTAYGGGTNISNLNQDILSRLEVPFPKPTE
                     QRAIAGVLSAYDDLIENNTRRIQLLERMAQALYREWFVHFRFPGHAQVKRVASPLGPI
                     PQGWEAGKLGHHLIALETGKRPKGGVGDLTNGVPSIGAENIKGIGRHDFASEKYVSRK
                     FFEEMHKGVVQDRDVAIYKDGAYIGRSSYLRDGFPHHECCVNEHVFLLRSSGHRLTQN
                     TLYLWLQEPDTVQAIRGTNANAAQPGINQQSINGLGLLAPPAEIAAEFDLVVEPLLAG
                     IVNSATRNKVLRRTRDLLLPKLISGVLDVSKLASETA"
     gene            complement(3849..5443)
                     /locus_tag="PKK20_08150"
                     /pseudo
     CDS             complement(3849..5443)
                     /locus_tag="PKK20_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            complement(5570..5830)
                     /locus_tag="PKK20_08155"
     CDS             complement(5570..5830)
                     /locus_tag="PKK20_08155"
                     /inference="COORDINATES: protein motif:HMM:NF017843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNU99899.1"
                     /translation="MNGTPNWYCSNSHDTGCAYQLHAGIYRRICANGLVLSSSAFEAV
                     RFRHTGLVAEEVVQAKCNGLQATEASCLHMSSVADLQIRTEA"
BASE COUNT         1171 a         2028 c         1818 g         1335 t
ORIGIN      
        1 cacgatttac cccagtggcc gtttcgagtc cagcaaaaaa atgtccaaac tccagacccc
       61 tctcccactg ggagaggggc cgggggtgag ggtcgcaagc agcgcgttga tggcgttgtt
      121 gacgccgagg tactggtgat tcttggcgac caccttcctg atcttccccg ccttctcttc
      181 gaacagggtg aagttctcga ccaagtccag cagccgggtc ttgtcacacg tgcctttcaa
      241 gagcgtttcc aaagacacgc tgcccggctc cgcctcgctg ctgatcttct tccaatcgca
      301 gtaatgctcc cactcagccg tcaggctgcc caagcggctc tcgacgccgt tcgacacgag
      361 gatgaaggcg ttgctccaga acaactgcgg gatcgtggtc ttgtagtcgg tgatgttgtc
      421 cttgaacgcc gcctcgacgc gctccgtcac cgccttgaac tcactgacca ccagcggcag
      481 gccgttgacg aaccccacca agtccgggcg gcgcttgtac atctcgcccg tcacccacaa
      541 ctgcgacacc aacaggaagt gattgttcgc cgggttgtcc cagtcaatga ccctgatccg
      601 ctccggcacc aactcaccct ccgggttacg gatttccacc ttcacgccgt tcttgagcag
      661 cgcgtaaacc tcccggttgg cggcggccag cgacatcacg ctgcggtcgc gggtcaaatc
      721 gtttacagcg gcatcaatcg cctccggcgg cgcttgggga ttcagcttct tcagtgccgg
      781 gaccagatgc cgcagcaaca cgacctcgcc cgcgctttcc cggcccagcg tgccgccggt
      841 gccgaagacc tcatcgaagg cgccgatgct ctgccagccc aacccggcca aaagctcgat
      901 ggcgggctgc tcgaccaacg tgtcttcgga gtagcccttg ctcatatcgt cgcctcatca
      961 cgcccaaaag ggcacgtact cggcgaactt cttagacttg ttgcttgggt cggcggcttt
     1021 gaccagattc ttgtcgatgg cctcttgtat caaaaccgac accattgacc tctgcttctc
     1081 cgacatcttc aaccgttcgc gcagggtctt gttggtcatg gcgctgtcgg aataatactt
     1141 caagaccgcg tgctgatagc aggcttccaa ccgctcacgg accgacatct gggcgaacgt
     1201 ccggggcgtg aacagcgtga ccttgaagta attcgacccc gccgtgaact caatcggtgg
     1261 aagtccgtag agttccacct gtatccctgc cttgagcagt cctgaccccc gttcctcgca
     1321 gatcttgtag cgccgaaacg cccgcgccag ttgctcattg cgggactccg gctgtgtgcc
     1381 aatcagccgg tcaagccgct tggacggcaa tagcccgccg ggattcgaca cttcaatgcg
     1441 gtcgtcgaag atctcaatca aaggccccgc gcccgtgatg gaaaaatcct ggtgaatgag
     1501 cgcgttggcg atgagctcgc gcagcgcaat ttccgggtac accgtgcgct tccgacgcag
     1561 cgcctgctcg atgacctcgc tctgcggcag caacgacatg acgaattgca gcagcccttg
     1621 gaagctgatc gcgtagccct tcgttccttc ccgctcctgc ttcgtcttcg ttttgttcaa
     1681 cccctcatag acgaccaccc gcaccgcctt ccggctcagg tcaggataat cggcgagccg
     1741 ccgcgctgcc gcgatagcgc cgagattcgt cacgtagcca ccgcccgcag gttcgcggac
     1801 gatgaatcgc tcggattcca tccacgccag cgtttcctcc gctgtggtgg gcacaggccg
     1861 ctccagcatc ttgtggatcg gctcgatatc cagtctttgc gcgaatacac cgtccggcaa
     1921 cagcaccgac gcgtgcagtt cttcccagcg cggcgtccga ctgttcagca tcaacgtccc
     1981 gatctcctga cgtgaagcct tgcgggttgt gccgccggaa cggatgaacg cttcttccag
     2041 tcccttgcct cgtagatgca ctggcttcac cgccgactcc ggcacgtgca caaacagcaa
     2101 ccgcaccgag ccgtaatctt ccaccgcatg atcgagcgcc accggcggct caagcccctg
     2161 ccgaccgaga ttcgccagtt ggttcaccgt tgtctcgacc gtcttctcgt ccaccccgct
     2221 ggccgttccc gtcggatcca cgccgaacac cagataaccg ccgcccggct ggttcgcgaa
     2281 cgccgacaga tgttccgtca gccgcttctt gtcgggggac agccccgcct tccaatcgca
     2341 ctcgttcaat tcgtgcttgg gcggttcaag gctggccctg agcaagtcca gtgctttggt
     2401 catccaagct ttcatcggac gttttctact caaataaaaa catgctgcaa atgatggact
     2461 tacgccgact gcccgtccat ccatcaaata agcccggcag cactggcacg ctcccagcca
     2521 acccaaacgc gcccgcatcg gcgttgcctt tgacgctcat gcggtttcgg aggccagctt
     2581 cgacacgtcc aacacgccgg aaatcagctt gggcaacagc aggtcgcggg tccggcggag
     2641 cactttgttt cgtgtcgcgc tgttgacaat ccccgccagc aaaggctcaa ccacgaggtc
     2701 aaactctgct gcaatctctg ccgggggcgc cagcaaacca aggccgttga tagactgttg
     2761 gttgatgccc ggttgggctg catttgcgtt cgtaccgcga atggcttgaa cggtgtcggg
     2821 ttcttgaagc cacaggtaga gcgtgttttg cgtcaggcga tgcccagatg aacgaagcag
     2881 aaatacatgc tcgttcacgc agcactcgtg gtggggaaaa ccatcgcgga ggtacgaaga
     2941 ccgcccaatg taagcgccgt ctttgtagat cgccacgtct ctgtcctgaa ccacgccttt
     3001 gtgcatctcc tcgaagaact tccggctgac gtatttctcg ctggcaaaat cgtgccgtcc
     3061 gatgcccttg atgttctccg ccccgatact tggcacaccg ttcgtcaaat ctcccacacc
     3121 acccttcggc ctcttgccgg tttccagcgc gatcaagtga tggcccagtt tacctgcttc
     3181 ccacccctgc gggattgggc cgagcgggga ggcgacgcgt ttgacttggg cgtggccggg
     3241 gaagcggaag tggacgaacc actcacggta gagcgcctgc gccatccgct ccaagagctg
     3301 tatccgccgc gtgttgttct caatcaggtc gtcgtaggcg gagagcacac cagcgatggc
     3361 gcgttgttcg gtaggtttgg gaaacggcac ttccaatcga ctcaaaatgt cctggttgag
     3421 gttggagatg ttcgtgccgc ctccgtaggc cgtcagtcct ttgcggatga tgggcgtacg
     3481 gaaaacgtaa gcatagaaac gcgaaacggc ctctggcgat gtaaaccgaa ggcggatggt
     3541 gaaggccgag tgagtgacct gttcttgcag gtcatcaatg tacaagctgc ggccaatcaa
     3601 ctcgcggttg ccatttgacc gcacgaaaac gatgtcgcct ttctggagga cgttgcgctc
     3661 cgtgacgatg ccctccgggt tgatctgctc aagttccgcg tagagcggtt tcgtgtagtt
     3721 ctgaaaatcc ttaacaccca cgaccttgat gccttggcca aaactacgct tgtcgtagtt
     3781 gacgccattg cggaattcgg caaggtcgcc aagtagtttt gtgggccact ttgctctcgc
     3841 cgccatcgtc acgcctccag aatcttggcc gcgttgccag cgatccgttc ctccagttcc
     3901 cgcgcctcag cgttgagcgt ttccagttct tcggtcagtt cctcgaactg ctccctgaag
     3961 tcggcgtcgc tgacttcctc acccgccgcc acgcccacgt tgcggcccgg attgaggctc
     4021 cagccctgcg cctcgatttc cttccgggtc gccactttgc acaggcccgc cacgtcggcg
     4081 tacctgagct tggggaactt ttccttgagc agtttttcgg acccgcgcac gaactccggc
     4141 tccttgcccc gatacagccg cacgatgttg gccaaaaact cgacctgctc cggtgtgaac
     4201 tcgcggtggg cgcggtcaat ctgccggtag atgtgccggg catccacgaa cagcacggtg
     4261 tcctgatttg cggtcatcgg cgccgctggc gatttgggag gcggcaccgt cgccttgccc
     4321 cggtcgaaga accagagcgt cgcgggcagc gtgaccgtgt aaaaaaagtt cggcccgacg
     4381 gacacgatca catccaccgc gccagactgg atcagcttct tgcggatttc cagttcggac
     4441 ccgcgagcat ccgccgccga gttggccatg acgaacccgg cgcggccctt ggggctgagc
     4501 gcgtggtaga aaacctgaat ccagaggtaa ttcgcgatgt cggtggtggg caggccaagg
     4561 cggaaacgcg ggtcatcctt gagtttctct ttgtccacgc ccgagacgtt gaagggcgga
     4621 ttcgccatca cgaagtcgaa tcggcccatg cagtcgtgaa cgtcttcgta gtacgtgttg
     4681 gcctgccgga tgtcgccgct caagccgtgg cgaggttcat tttgcacagc cggatggtct
     4741 ccgccacacg ctcctggccg tagatggcga tttcctcgct gggcttcctt cggtggttgg
     4801 ccacgtaacg ggcgctggac acgaacatgc cgccgctgcc gctggccgga tcgaacagcc
     4861 gcccgtgaaa cggctcgatg atctcgacga tcagatcgac catgctgggc ggggtgaaga
     4921 actcgccgcc tttctgcccc tctttcatgg cgaacgcgcc caagaagtac tcgtagattt
     4981 tcccgaaggc gtcgccgggc acgttcaggg gaatgctgga catgatcttg agcagttcga
     5041 ccagcgtggc gttgtcgatc cgctggtagg acttgggcag cacatccttg aggtcgtcgt
     5101 tctcctgctc gatggccttc atcgcctcgt tgagcttctt gccgatgtcg gcggactccg
     5161 gcagttgcag gagccgagag aaccgcgctc cctcgggcag aaagactact cctcgcgcct
     5221 gatagtccgt cttgccgatg gtgcgccgcc cggtggactt gcccttgagt tcggcctcga
     5281 ccctggtgaa ggcgttgtcg gcaaaccgca ggaagatgag gcccaggaca ggggtcgagt
     5341 attcggaggg gcgcagcggc gaattggccc agagttggtc agcagcagcc cagaggcgct
     5401 tctccaactc ggcaagaccg ttcgcggttt tcggctcagg catagcgagc gacaccgccc
     5461 gcgagggcaa gctgtcgcgg atcagcgtca cgctgtttat cggggaaacc ggcgagagtg
     5521 gcaacccgaa gtttaagacg atgggcgact gttcctcaga cggattcagt caggcttcgg
     5581 tgcgaatctg caagtctgcg acgcttgaca tgtggaggca actggcttca gtggcctgca
     5641 aaccattgca cttcgcttgc acgacttcct cggccacgag gccggtgtga cggaagcgga
     5701 cggcttcgaa cgccgaactg gaaagcacca acccattggc gcagatgcgt cggtagattc
     5761 cggcgtggag ctgataagcg cacccggtat cgtgtgagtt tgagcagtac caattcggtg
     5821 ttccattcat cgagcgtctc catctgttca gcccggcgga agcggatgag atgcttctga
     5881 aagccccgcc gggcttcgtt gcggattcgt tgttcctcca ccgccaccgg aacccaatcg
     5941 tgctcgcgca agccgtccac aatccgggcg ggtcggcacg aacgcgtagc ggtgactctc
     6001 cccatcggcc cgtggcaaag atgctcgggg cctgggcgcg gaggttttcc tcggtcacat
     6061 taacttcctg ggcagatact cctttgccct tgcagatccg gtcgcacggg ggggaactcc
     6121 gacctttacg ggacccggac gacagcctgg gcttagcgca agtttccgct ccaatgattg
     6181 tcggaggccc atttgcccca accctaaacc ccgaaaccga ggtcgccgtc tccgccgtcc
     6241 gggtcgttgc tgatgccggt cacagaccgg cagatcccga cgtagccttc cagtcgccct
     6301 agcagcctgt cggacttttc cccaacaatt ttgcccggcg tttgaacaag ag
//
LOCUS       DAOQRO010000191         6351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12236_length_6351_cov_5.111499, whole genome shotgun sequence.
ACCESSION   DAOQRO010000191 DAOQRO010000000
VERSION     DAOQRO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12236_length_6351_cov_5.111499"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..2177
                     /locus_tag="PKK20_08160"
     CDS             279..2177
                     /locus_tag="PKK20_08160"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNU99900.1"
                     /translation="MAEPKTNELMEKIVSLCKRRGFIFQSSEIYGGINGFWDYGPLGA
                     DLKRNVRDAWWRTMVQQRDDVVGLDATIIMHPDIWKASGHVDTFSDPMCDCLLTQKRF
                     RADQVEPQSGDVHRYAGAFGGGWLEAVAQAGVKDVPETFVGGYRTLTVDEAARLLDAV
                     KGAGLNPTWHVDASFSVLIPAGKHPEYANKVGRAFYAARGLRNPVLILAGTESVRDSM
                     RYNPENGAELTEPRPFNLMLQTCVGPVQSAENVAYLRPETAQAIFAQFKNVLETSRQR
                     VPFGIAQVGKAFRNEVTPRNFTFRSREFEQMELEFFIKPDEAIEAICGHVARVEDGAW
                     QGEPQPDWGWEVWHKYWVEQRIRFFESVGLPRASLEEYWQKPEELAHYARATVDLLYQ
                     FPFGAQELEGIAARSDFDLSQHQRFAGKPMTVFDEELKVAWTKLDQNRKDDLWRRYFE
                     GRRSYLSRTGLEAEKAVSQARLEADALAKGWYVPHVIEPSAGVDRLILALVSHAFTEV
                     ESVDDKGKKETRLIMRFHPRVAPIKVGVFPLLKNKPDLVRTARAVRDLLRPHLMVFYD
                     ETGSIGRRYARQDEAGTPFGVTVDFDTLGEGGPELRDTVTVRERDTGQQVRVKIDALL
                     PWLLERVR"
     gene            2248..2741
                     /locus_tag="PKK20_08165"
                     /pseudo
     CDS             2248..2741
                     /locus_tag="PKK20_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126374.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2924..3646)
                     /locus_tag="PKK20_08170"
     CDS             complement(2924..3646)
                     /locus_tag="PKK20_08170"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99901.1"
                     /translation="MLNRSRSMTLPHHLRFAMGLIAAFVVAGCRTADQSAGWRPLFPE
                     DGVPQGWLVRHWADLRNPPPADTEWRVSGGVLSNTGSRGTWLISEAEYGDFDLDYEFK
                     LGERGNSGCALRAPLFGDPAFDGLELQMVDLRYNPEATPSELTGSLYRAVAPRRQCYR
                     ATDWNRYQITCRGPHIRVLLNGEQILDVNLDAQTASAKRHNGEEAPALRLRPRRGHIG
                     FQELSRDDVRVQVRNARIRPLD"
     gene            complement(3661..5466)
                     /gene="rpoD"
                     /locus_tag="PKK20_08175"
     CDS             complement(3661..5466)
                     /gene="rpoD"
                     /locus_tag="PKK20_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNU99902.1"
                     /translation="MAKARAKSRKPKAEVVFAPSLPVLPVPARVAALTGEASAVVAAP
                     SGTGTGGEARPPGGFDLSEKIKELVRLAQEQGYLTYNDINDALPDAVVTADDLDEVYT
                     RLRNLDVEIVDQAEVDRIKQPEPEEEDEKARLDILDDPVRMYLKQMGQVPLLTREQEV
                     EISKRIEDAENEVKRIIYGFGFAGKEHIALAEKLISEPPKERFDRVILDKKIEGRENH
                     LRALRKLVKDVRGVDQHVDGVYARWQKESNKSRKLKASSEFRKMDNKLQATFPKFYYK
                     QKVIEEMALVADNIHDKIQMSLRAVMELESQRKPAHQSIVDSERRKIQALEEFVRMSH
                     TDYLNAYKQLQHFTYKAHQAKTEMVEANLRLVISIAKKYTNRGLSFLDLIQEGNMGLM
                     KAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQKQLV
                     QDFGREATPEEIADEMQMPVERVRAVLKMAQQPISLQSPVGDSDDTNFGDFIEDKSAE
                     NPSDMTSYSLLKDKLGQVLETLTERERKVLELRFGLGDGYSRTLEEVGKQFKVTRERI
                     RQIEAKALRKMRHPTRIRQLQGFLETEEVEAAVAP"
     gene            complement(5976..>6351)
                     /locus_tag="PKK20_08180"
     CDS             complement(5976..>6351)
                     /locus_tag="PKK20_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mucin desulfatase"
                     /protein_id="HNU99903.1"
                     /translation="TVMPGLVHYDFGDMVRTTTSPTLEDEKDLSKVEMQMPLFQALAQ
                     GYMKAVEGFCTPAERRYLAFSGKLITYTIGIRFLADYLMGDNYFRVHRPDHNLDRCRT
                     QFRLVESIAQQEEAMQKFVDSL"
BASE COUNT         1138 a         2036 c         1987 g         1190 t
ORIGIN      
        1 gacaggccgc tgcgctttgc ggcgcaggca gggcacgcgg cctacaccgg gagtcgcgat
       61 ccggtgtagg cccggtgccc tcaccgggcg gcatcacacc gacaacgagc agatgcgccg
      121 ctggaaggcc tcatcttgaa cgcagcgtct tgccgccagg cgaggccacc cggcccgcgg
      181 gaacggagaa agcagcaatt gcggacgggg ggccattggg ggtgcctatc gggggtgcct
      241 tgacatcggg acttcctttc caaagactgc cccgctttat ggccgagccg aaaacgaacg
      301 agttgatgga gaagatcgtc tccctgtgca aacgccgcgg tttcattttt cagtcgtctg
      361 aaatctacgg cggaatcaac ggcttttggg actatggacc gctgggggcc gatctcaaac
      421 gcaacgtccg cgacgcctgg tggcggacga tggttcaaca gcgcgacgat gtcgtgggcc
      481 tcgacgcgac catcatcatg catccggaca tctggaaggc ttccgggcac gtcgacacgt
      541 tcagtgaccc gatgtgcgat tgccttctga cgcaaaagcg gttccgtgcg gaccaggtcg
      601 agcctcagag cggcgacgtg caccgttatg ccggcgcctt cggcggcggc tggctcgaag
      661 ccgtggccca ggccggagtc aaagacgtcc ccgaaacgtt cgtgggcggg taccggacgc
      721 tgaccgtgga cgaggcggca cgcctcctgg atgcggtgaa gggcgccggg ctcaatccga
      781 cctggcacgt cgacgcatca ttctccgtgc tcattcccgc aggcaagcac ccggagtacg
      841 cgaacaaggt gggacgcgcc ttctatgcgg cgcgcgggct gagaaatccg gtcctgatcc
      901 tggcaggcac cgaatcggtc cgggactcga tgcgctacaa tccggagaac ggcgcggaac
      961 tgaccgagcc gcgcccgttc aacctgatgc tccagacatg cgtggggccg gtccagagcg
     1021 cggagaacgt ggcgtacctg cgccctgaga ccgcccaggc gatcttcgcc cagttcaaga
     1081 atgtcctcga aacctcgagg caacgggtgc cgttcggcat cgcccaagtc ggcaaggcct
     1141 ttcgcaacga ggtcacgccg cggaatttca ccttccgatc ccgcgagttc gagcagatgg
     1201 aactggagtt tttcatcaag ccggacgaag ccatcgaggc gatttgcggg catgtcgccc
     1261 gggtggagga cggggcctgg cagggcgagc cgcagcccga ctggggctgg gaagtctggc
     1321 acaagtactg ggtcgagcag cgcatccggt tcttcgaatc ggtcggcctg ccgcgcgcct
     1381 cgctcgaaga atactggcaa aagcccgagg aactggccca ctacgcgcgg gccacagtgg
     1441 acctgctcta ccagttcccg ttcggggccc aggaactcga gggcatcgcc gcgcgcagcg
     1501 atttcgatct ctcgcagcac cagcgattcg ccggcaagcc catgacggtg ttcgacgagg
     1561 agttgaaggt cgcctggacg aagctcgatc agaatcgaaa ggacgacctg tggcggcgct
     1621 acttcgaggg gcggcgcagc tatctctcga ggacgggcct tgaagccgag aaagcggtca
     1681 gccaggcgcg cctcgaggcc gatgcgctgg ccaagggctg gtatgtcccc catgtgatcg
     1741 agccgtccgc gggtgtcgac cgcctgatcc tcgccctcgt ctcccatgcc ttcacggaag
     1801 tcgagagtgt cgacgacaag ggcaagaagg aaacgcggct gatcatgcgc ttccaccccc
     1861 gcgtggcccc catcaaggtc ggggtctttc ccctcctcaa gaacaagccg gacctggtcc
     1921 gcaccgcccg ggcggtccgc gacctcttgc gtccgcatct gatggtcttt tacgacgaaa
     1981 cgggatcgat cggacgccgc tatgcgcgcc aggatgaagc gggtacgccg tttggcgtga
     2041 cggtcgattt cgacacgctc ggcgagggcg ggcccgaact ccgggacacc gtgacggtgc
     2101 gcgagcggga cacggggcaa caggtgcgag tgaagatcga cgccctcctg ccctggcttc
     2161 tcgagcgggt gcgatagaca tggccgtgcg ccgggccctt cttccattgc catgacaccg
     2221 gaacaaccgg ccaaggcgaa agcgctcggt ttcgcggaca aacaactggc cttcctgacc
     2281 ggacctgcgc gggatgcgcg cccggcttgg cttggtctca agctatcggc tggtggacac
     2341 gtgcgcggcg cagttcgaag cccacacatc ctattactac tcgacgtatg accgcggcga
     2401 cgacgaggtc aagcgctcgg atcggcgcaa ggtgatgatc ctgggcgggg gggccgaacc
     2461 gcatcggcca gggtatcgag tttgactact gctgcgtcca cgctgcttcg ccctcaagga
     2521 ggaacgtcca atacgccgtc aagggcgagc gcatctatgt gctcgaagtc aatccccgag
     2581 cctcccgaac cgtgccgttc gtgagcaagg ccagcggcgt gccgctggcc aagttggccg
     2641 cccgagtcat ggccggacgc actctggccg aactcggatt cactgccgag acctggccgg
     2701 accattgggt cgtcaaggag tctgtcttcc ccttcaaccg gatcagaatc cgcaccaccg
     2761 ccgtctatac cggcatcccg attatgacca ccttgagcgg cgcccgcgcg gcgattctgc
     2821 gcatcgcggc cctcaaacgc cgcggctgtg gaggcaaagc tctccaggaa tactcctgaa
     2881 accccgccgc ccctcgactg cgtccgggcc cgggcggatt cgctcaatcg agcggacgga
     2941 ttcgcgcatt ccggacctgg acccgcacgt cgtcgcggct cagctcctga aatccgatgt
     3001 gcccccgacg cggacggagg cgcaacgcag gggcctcttc gccgttgtgc cgcttggccg
     3061 acgccgtctg ggcgtcgaga ttcacatcca gaatctgttc accgttcagg aggacgcgaa
     3121 tgtgcgggcc gcgacaggtg atctggtagc ggttccaatc ggtggcacga tagcattggc
     3181 gccgcggtgc gaccgcgcgg tagagactgc cggtcaactc cgacggggtg gcctccgggt
     3241 tgtaacggag gtcgaccatc tgcaattcca acccgtcgaa ggccgggtcg ccgaacaggg
     3301 gggcgcgcag cgcgcagccg ctgttgccgc gctcgcccag tttgaactca taatcgagat
     3361 cgaagtctcc gtactcggct tcgctgatca gccacgttcc gcgtgatccc gtgttgctca
     3421 agacaccgcc gctcacccgc cactcggtat cagccggagg cggattgcgc agatcggccc
     3481 agtgacgcac cagccagccc tgcggcacgc cgtcctcggg aaacaacggt ctccacccgg
     3541 cggactggtc ggcagtgcgg caaccggcca cgacgaacgc ggcgatcagg cccatcgcga
     3601 atcgaaggtg atgaggcaga gtcatcgagc gggaacgatt gagcatcggg ccaggggctg
     3661 ttatggcgcg acggccgctt ccacttcctc ggtctcgaga aagccctgca actgccggat
     3721 tcgggtcgga tggcgcatct tgcgcagcgc cttggcctcg atctggcgga tgcgttcgcg
     3781 tgtcaccttg aactgcttgc ccacctcttc gagggtgcgg gaatagccgt cgcccaggcc
     3841 aaagcgcaat tcgagcacct tgcgctcccg ctcggtcagc gtctcgagca cctgccccag
     3901 cttgtccttc agaaggctgt agctggtcat gtccgaagga ttctcggccg acttgtcctc
     3961 gatgaaatcc ccgaagttgg tgtcgtcgct gtcgcccaca ggcgactgca gcgagatcgg
     4021 ctgctgagcc atcttgagaa cggcacgcac ccgctcgacc ggcatctgca tctcgtcggc
     4081 aatctcctcc ggggtcgcct cgcgtccgaa gtcctggacc aactgcttct gaacccgcat
     4141 cagcttgttg atggtctcga tcatgtgcac cgggatgcgg atggtgcggg cctggtcggc
     4201 tatcgagcgg gtgatggcct ggcgaatcca ccacgtggcg tatgtggaga acttgtagcc
     4261 ccggcggtac tcgaacttct cgacggcctt catcaaaccc atgttgcctt cctgaatcag
     4321 atcgaggaaa gagaggccgc ggttggtgta cttcttggcg atggagatca ccagacgcag
     4381 gttggcctcc accatttccg tcttggcctg gtgcgccttg tacgtgaagt gctgaagctg
     4441 cttgtaggca ttgagatagt ccgtgtggct catccggacg aactcttcga gcgcctggat
     4501 cttccgacgt tcggaatcga cgatcgactg gtgggcgggc ttgcgctgcg actcgagttc
     4561 catgaccgcc ctgaggctca tctggatctt gtcgtggatg ttgtccgcca ccagcgccat
     4621 ctcctcgatc accttctgct tgtagtaaaa cttggggaat gtcgcctgga gcttgttgtc
     4681 cattttccgg aactcggacg acgccttcag cttccggctc ttgttgctct ccttctgcca
     4741 ccgcgcatag accccgtcca catgctggtc cacccctcgg acatccttca cgagcttgcg
     4801 cagggcgcgc aggtggttct cccgaccctc gatcttcttg tccagaatca cccggtcgaa
     4861 acgctccttg ggtggctcgg agatcagctt ctcggccagg gcaatgtgct ccttgcccgc
     4921 aaaaccaaag ccgtaaatga tccgcttgac ctcgttctcc gcatcttcga tccgcttcga
     4981 aatctccacc tcctgctccc gcgtcaaaag cggaacctgc cccatctgct tcaaatacat
     5041 ccgaaccggg tcgtcgagga tgtccaaacg cgccttctca tcctcttctt ccggttccgg
     5101 ctgcttgatc cggtccacct ccgcctggtc cacaatctcc acatccaggt tccgcaggcg
     5161 ggtatagacc tcgtccagat catccgcggt caccaccgcg tccggcagcg catcgttgat
     5221 gtcgttgtag gtcaggtagc cctgctcttg agccagccgt accaattcct tgatcttctc
     5281 cgacaagtcg aaaccgcctg gaggacgcgc ctcgccgccc gtgccagtgc ccgaaggcgc
     5341 agccacaaca gccgacgcct cgccggtcag cgccgcgacc cgtgccggaa ccggaagcac
     5401 cggcagcgac ggcgcaaaga cgacctccgc cttcggtttt ctggacttgg cacgcgcttt
     5461 ggccacgcga cggtggccgg cggatttgga atggcctttg ggggaacggt gtgcgacggc
     5521 agctttcttg cgcttctgtc tcgacataat gtttggatag atcaacccag cccgggcgaa
     5581 atcaagatgg cccaacacta ttgtgtttgt gcctccctta gtcaagcggc agatgccgcc
     5641 agacggcgcg ggcgcggcga cacggttgtt ctgttcaaat tcctgaaggg ttgctgttgt
     5701 tgaggttggc tctgctgcaa gtgaaaagac cgtgggcgcg gcggggggac cccagtctcc
     5761 ttttgactgg ctcgtcgatt cgcccttgcc aagaacaggc cgcagccgga ccgcgttgat
     5821 cgactggtcg gggcagcgcg gaaggggcgg agcgagtccg gaacggctgg gacgccgcga
     5881 tcgccgcggt catgggtcgg tgccgcatgc tctgccgcac ctccccctac cccaggccta
     5941 aaccatccag aatgcagaat cgcagggggg ccggttcaaa gactgtccac gaacttctgc
     6001 atcgcctctt cctgctgagc gatggactcg accaggcgga actgggtgcg gcagcggtcg
     6061 aggttgtgat cgggacggtg tactcggaag tagttgtcgc ccatgaggta atccgccaga
     6121 aagcggatgc cgatcgtgta ggtgatcagt ttgcccgaga acgccagata ccgacgctcg
     6181 gccggggtgc agaatccctc gaccgccttc atgtatccct gcgccagcgc ctgaaacaag
     6241 ggcatctgca tctctacttt ggaaaggtcc ttctcgtcct cgagagtggg gctggtcgtg
     6301 gttcgcacca tgtcaccgaa gtcataatgc accaggcccg gcatgacggt g
//
LOCUS       DAOQRO010000192         6344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12257_length_6344_cov_4.817459, whole genome shotgun sequence.
ACCESSION   DAOQRO010000192 DAOQRO010000000
VERSION     DAOQRO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12257_length_6344_cov_4.817459"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1083)
                     /locus_tag="PKK20_08185"
     CDS             complement(76..1083)
                     /locus_tag="PKK20_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU99904.1"
                     /translation="MSAIDVQNLTKTFRAYKKQPGLKGAVKGLFRRVSETVVAVKDVS
                     FEIDEGELVGFLGPNGAGKTTTLKMLAGLMYPVSGTARVLGYVPWERKDEYRRQFALL
                     LGQKNQLWWDLPARESLELNAHIYGIDAASFRKTVAELVEMLDVKDKLNVMVRELSLG
                     ERMKFELIASLLHQPKVLFLDEPTIGLDVVSQKTVREFLRHHNATRRTTILLTSHYMA
                     DIEALCRRVIIIDHGAIFFDGQLSAVLDRFADFKLVTIRLAGDATIGADLSAYGDVVQ
                     RTSATAQFKVKRDRVIAVCKTLLDQLPVADIDIQEVPIEDVIGQIFARRPVADQNLFF
                     R"
     gene            complement(1294..3570)
                     /locus_tag="PKK20_08190"
     CDS             complement(1294..3570)
                     /locus_tag="PKK20_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99905.1"
                     /translation="MIALLGVFTMACGIAVGANAGTLAGYLRDQNWYARYQSQPYGVG
                     YYEYGINANPDGAAGTGGAAATDVYGRFSMGSLAAGLYTVVSWDVWWRSSYAFNVSVP
                     AGGSSPSVDLRLKATMWGYPAFWDDTGYHEFGQTFTATGPISMIYLRAPFSTSYTLTI
                     HADGPGGPQVGAARTFSGGDQRPIYAYGDMPTVAGRTYYARIRTSSPSIGGVLMQMDP
                     RPDFSDPMPGGCLWLGNGSVLVPYPDRDLGLVIMSDDDGLITNLHARSSGAPLDNANS
                     VGQTFIARGTALISAAFWLADPAAPTYEVRVFEGGPGGGQVGTAKRGRPPRVTADPEM
                     IVAWTPGECPLTPGQVYYVEVTRAGGGSFSRIYVNRANPFPHGQGHQNGVAVSGADLA
                     GTLMEEAAQGAATQSAVRFVTEPAVAEAERESDRFVIRWRTDTASDSQVEYAPGNPPY
                     TGLATDANLVLDHALTLSGLQAQTLYHLRITSAAPGHRTGVSRDLALCTRPTRPNLLT
                     NPGFESGSGSSPRKTIPGWTKSGGLDMGMSDGTWFGELKPHTGQWLLEGALNGSSSDS
                     HLYQRVSVTPGKRYTFSAWVFTKALERINNQTVEKYDVWNDRNRLIHVRLGLDPLGGT
                     NPNASSVRWTPRLYSHLRYSNPALATVASNTTLTAFVHFKGEGVEWHLYGIDDCVLTE
                     TDPPPPLLIAPRLDLDGSFSCQVGSEAGGWVRIEGSSDLGSWVALTNMLNATGLTRFT
                     DFGGAPRRFYRASAPASP"
     gene            complement(3784..4035)
                     /locus_tag="PKK20_08195"
     CDS             complement(3784..4035)
                     /locus_tag="PKK20_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99906.1"
                     /translation="MSSAIVRARLQATPFRPFELWLNSGQVVRVPHPDFAFMPPKPNQ
                     WDVVVYDENRTFSIVDLGQVAALKEVRPGKANPKGPCAK"
     gene            complement(4249..5529)
                     /locus_tag="PKK20_08200"
     CDS             complement(4249..5529)
                     /locus_tag="PKK20_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNU99907.1"
                     /translation="MNRPAAPRASPACSNSDASPRAPFEQAAHPRLKLAVSKIIVRGV
                     NWLGDAVMSTPALLQLRAAYPNAHIALLTPAKLADLWMHHPAIDAVLPLEPHASPWQT
                     GRQLRQHRFDLGILLPNSPRSALELWWGRVPRRMGYATPWRRWLLTEAVAQRPGHVAM
                     RKRSTSEIRSLITGGSPAHAGPSWPAAAHHIHHYLELVAAAGADPTPLAPHIAVTQGE
                     IEAAARRFGLPQPCENAVPVLGLNAGAEYGPSKRWPPDSFVAAALALRRRLGCHWVVF
                     GGHADVAQSASIAAAIAAQTDLSGQDSRADVPRVFNLAGRTSLRELCATLRLCRVLIT
                     NDTGPMHVAAAVGTPVVVPFGSTSPELTGPGLPKDPRHRLLCADVPCRPCFRRDCPID
                     FRCMRSISVERIVTAVIGLAAVARQSDIDDAAPG"
     gene            complement(5526..>6344)
                     /locus_tag="PKK20_08205"
     CDS             complement(5526..>6344)
                     /locus_tag="PKK20_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU99908.1"
                     /translation="HRRAAIENLTGCFGHEQSPRAIRALARENFRRIGETFACAVRTA
                     TMSDDEIRAILEVAGADAVLAAAQGHRGVVAAIGHFGNFELYARGSLWLDGYQSATTY
                     RALRQPSLNRLLQTLRARSGCLYFERRFETAALREAMNRQRLVVGFLADQHAGNRGLP
                     VPFFGRTCSTSAAPAVFALRYNCLLITAICFRTGLGQWRIELGPPIPTRLGGKARSVE
                     AIMLDVNCAFEAAVRRDPANWFWFHRRWKPGKHRAPSRSAPVADDHDTPNPPRA"
BASE COUNT         1106 a         1945 c         2202 g         1091 t
ORIGIN      
        1 cagcgagacc gagccgctcg tgatcttgcg ctatttcggg cctgaagtga atcctgaggc
       61 tccggagctg ggggcttacc ggaagaacaa gttttgatcc gccacggggc gccgggcgaa
      121 gatttggccg atgacgtctt cgatcgggac ttcctgaata tcgatgtcgg cgacgggcag
      181 ttggtcgagc agggtcttgc agacggcaat gaccctgtcg cgcttgacct tgaactgggc
      241 cgtcgcgctc gtgcgctgga ccacatcgcc gtaagcgctg aggtccgccc cgattgtcgc
      301 gtcgccggca aggcggatcg tcaccagttt gaagtcggcg aagcggtcga gcacggcgct
      361 gagttggccg tcgaagaaga tggcgccgtg gtcgatgatg atgacgcgcc ggcagagcgc
      421 ctcgatatcc gccatgtaat ggctggtcaa cagaatggtg gtgcggcgcg tggcgttgtg
      481 atggcggagg aattcgcgca ccgttttctg cgagacgaca tcgagcccga tcgtcggctc
      541 gtccagaaag agcaccttcg gctggtgcag caacgacgcg atcagctcga acttcatccg
      601 ttcgcccagg gacagctcgc ggaccatgac attgagcttg tccttgacat cgagcatctc
      661 gaccagttcg gcgacggtct ttcggaacga ggcggcgtcg atgccgtaga tgtgggcgtt
      721 gagttcgagc gattcccgag ccggcaaatc ccaccagagc tggttcttct ggcccaacaa
      781 cagggcgaat tgacgccggt actcatcttt gcgttcccat ggaacgtagc cgaggacccg
      841 ggccgtgccg ctgaccggat acatcagccc ggccaacatc ttgagggtcg tcgtcttgcc
      901 ggcgccgttg ggaccgagga agccgaccag ttccccttca tcgatttcga agctgacatc
      961 cttgaccgcg acgacagtct cgctcacccg ccgaaacagc cccttgaccg cccccttcaa
     1021 tccgggctgt ttcttgtagg cgcggaatgt ctttgtcaga ttctgaacgt cgatcgcaga
     1081 cacagggttc gagcttgccg ttcgcgacgc ggtcaagtcg agggaggaat tgtccgcgcg
     1141 aggttcatgg aagcgttgac attcagcggc ttaatgcgca tgggaacatg aaccggcttg
     1201 ccgccgacgt gagtcggcgc cgattcccgc ttctgacgga gcgagatggc gcggactgac
     1261 cccgcggcgg cgggacggct gcggaaccac ggttcatgga gacgctggcg cggatgcgcg
     1321 gtagaaccga cgcggcgcac cgccgaagtc ggtgaaacgc gtcaggcccg tggcgttgag
     1381 catgttggtc aacgccaccc acgagcccag gtcggatgaa ccctcgatcc gcacccatcc
     1441 gccggcttcg ctgcccacct ggcaggagaa cgagccgtcg aggtcgagtc tcggcgcgat
     1501 cagaagcggc ggcggcggat cggtctcggt caggacgcag tcatcgatcc cgtacaaatg
     1561 ccactcgacg ccctctccct tgaagtgaac gaaggcggtg agagtggtgt tcgaggccac
     1621 ggtcgcgagg gccgggttcg agtagcgcag gtggctgtag agccgcgggg tccaacgcac
     1681 actcgatgcg ttcgggtttg tgccgcccag cggatcgagg ccaaggcgga cgtggatcag
     1741 gcggttgcgg tcgttccaga catcatactt ctcgacggtc tggttgttga ttcgttcgag
     1801 agccttggtg aacacccagg cgctgaatgt gtaccgcttc ccaggcgtca ccgaaacgcg
     1861 ctggtacaga tgcgagtccg agctcgatcc gttgagcgcg ccttcgagca gccattggcc
     1921 agtgtgcggc ttcaattctc caaaccaggt cccgtccgac atccccatat cgagcccgcc
     1981 gctcttcgtc catcccggga tggtcttgcg cggactcgag cccgagccgg actcgaagcc
     2041 gggattggtg agcagattcg ggcgtgtcgg gcgggtgcag agggcgaggt cgcgagacac
     2101 gcccgtgcga tgccccggcg cggcggaggt gattcgaaga tgatagagcg tctgggcttg
     2161 gaggcccgag agcgtgagcg cgtggtccag aaccagattc gcatcggtgg ccagcccggt
     2221 gtaaggcggg tttccaggcg catactcgac ctggctgtca ctggctgtgt ccgtgcgcca
     2281 gcgtatgaca aaccgatctg actcgcgctc ggcttccgcc accgcgggtt ctgtgacaaa
     2341 ccggaccgcc gactgcgtcg cagccccttg ggcagcctcc tccatcagcg tgccggccag
     2401 atccgcaccg gagacggcga cgccgttttg atgcccctgc ccgtgaggaa atggattcgc
     2461 ccggttgaca tagattcggc tgaacgatcc cccgcccgcg cgcgtgactt cgacatagta
     2521 gacctggccc ggcgtcagcg ggcattcgcc cggggtccag gccacgatca tctcgggatc
     2581 ggcggtgact cggggcggtc tgccgcgttt tgcggttccg acctgtccgc cgcccgggcc
     2641 gccttcgaac acgcggactt cgtaagtggg ggccgcggga tcggcgagcc agaacgccgc
     2701 gctgatcagg gccgtgccgc gcgcgatgaa ggtttggccg acgctgttgg cgttgtccag
     2761 cggggcgccg ctcgatcggg cgtggaggtt cgtgatcagg ccgtcgtcgt cggacatgat
     2821 gacgagcccg agatcgcggt ccgggtaggg gacgaggacg ctcccattcc cgagccagag
     2881 acagcctccc ggcatcggat ccgagaaatc ggggcgcgga tccatctgca tgaggacgcc
     2941 cccgatactt ggcgaagaag tccggatgcg cgcgtagtat gtgcggccag ccacggtggg
     3001 catgtcgccg taggcgtaga tggggcgctg gtctccgccc gagaacgttc gggccgcacc
     3061 cacctgaggt ccgccgggac cgtctgcgtg gatcgtgagc gtgtaactgg tcgagaacgg
     3121 ggcccgcagg tagatcatgg aaatcgggcc tgtggccgta aacgtctgac caaactcgtg
     3181 gtaaccggtg tcgtcccaga acgccgggta tccccacatc gtggctttca ggcgcagatc
     3241 gaccgacggg ctcgaaccgc ctgccgggac gctcacgttg aaggcgtagg acgagcgcca
     3301 ccagacgtcc cacgacacca ccgtgtagag gccggcggcg agactcccca tgctgaaccg
     3361 accatagacg tccgtcgcgg ccgccccccc cgtgccggcc gcgccgtcgg gattggcgtt
     3421 gatgccgtat tcgtagtaac cgaccccata gggctggctt tggtatcggg cgtaccagtt
     3481 ctgatcgcgc aagtaacccg ccaaagtacc ggcgttcgcg ccgaccgcga tcccgcacgc
     3541 catcgtgaac acaccaagga gagctatcat atcagcatcg tgttacttag gaatcgctgg
     3601 gatgcactac accgtcaccc tcacccgtcc cgcatggcgg gacaccctct cccacctgcc
     3661 tgcgcccagg cttcggcagg caggtccgat gggagaggga cggggtgagg ggcctgtgtg
     3721 gcgtatccag ccgatcaggc agggaatcca ggcgccaacc tcgtccgctc gtccgcacgc
     3781 atgctacttc gcgcacgggc ctttcggatt cgctttgccg ggccgaactt ccttcagcgc
     3841 ggcaacctgc ccgagatcga cgatgctgaa cgtgcgattc tcgtcgtaga cgacgacgtc
     3901 ccactggttc ggtttgggcg gcatgaacgc aaagtccggg tggggaactc gcacaacttg
     3961 tccgctgttc agccacagtt cgaagggccg gaagggagtc gcttggagcc gggctctcac
     4021 gatcgctgaa ctcatatcgc gatcaaacta cacgcgcccc caccaactgc aaggaacgaa
     4081 tcggttcgga tcgctgcgcg gtgcatttga cagttgtcgg tattctgtcg cccggcgagg
     4141 gcaccgggcc tacaacggat ggagactccc gctgtaggcc gcatgccctg cgtgcgtcgc
     4201 agccgatcgt ctgtctgccg tgccggcata ggcaggcggc aggcaggcct accccggcgc
     4261 tgcatcatcg atgtcgctct gtcgagcgac cgcagccagg ccgattaccg ccgtcacaat
     4321 ccgctcgacg gagatggacc gcatgcagcg gaaatcgatc ggacaatcgc gtcggaaaca
     4381 ggggcggcag gggacgtccg cacacagcag ccgatgtctc gggtctttcg gcaggccggg
     4441 tccggtgagt tcgggcgacg tgctgccaaa cggcacgacc acgggtgtcc cgaccgctgc
     4501 ggccacatgc atgggacctg tgtcgttggt gatcagaaca cggcaaagcc ggagtgtggc
     4561 gcagagttcg cgcagggacg tgcggccggc caggttaaac acgcgcggga catcggccct
     4621 cgagtcctgg ccggacaggt cagtctgcgc cgcaatcgct gcggcaatcg acgccgattg
     4681 tgcgacatcg gcatgtccgc cgaacacgac ccaatgacag cccaggcgtc gccgcagggc
     4741 aagcgcggcc gcgacgaagc tgtctggggg ccaacgtttt gacgggccgt actcggcgcc
     4801 ggcattgagt ccaagcacgg gaaccgcgtt ttcacagggc tgaggcagtc cgaaccgacg
     4861 ggccgcagcc tcgatttcgc cctgcgtcac ggcgatgtgc ggggccagcg gggttggatc
     4921 ggccccggca gcagcgacca attcgagata gtgatggatg tggtgggccg cggcgggcca
     4981 cgaggggccg gcgtgcgcag gggagccgcc ggtgatgaga gaacggattt ccgatgtcga
     5041 ccgtttgcgc atggccacat gaccggggcg ctgggccacc gcctcggtca gcagccagcg
     5101 ccgccatggt gttgcgtaac ccatgcggcg gggcactcgt ccccaccata gttccaacgc
     5161 cgagcgcggc gagttgggca aaaggatgcc gagatcgaac cgatgctggc gaagctggcg
     5221 tcctgtctgc cagggggacg cgtgcggttc gagtggaagg acggcatcga ttgccgggtg
     5281 atgcatccac aagtcggcga gttttgccgg ggtcaagagc gcgatgtggg cgttcggata
     5341 tgcggcgcgg agttggagga gcgccggggt cgacatgacg gcgtctccca gccaattgac
     5401 gccgcgcaca ataatcttcg agactgcgag cttgaggcgc gggtgcgccg cttgctcgaa
     5461 gggcgcccgg ggagacgcgt ccgagttcga gcaggctggg gatgcccggg gtgcggcggg
     5521 ccggttcatg cacgcggggg gttgggtgtg tcgtggtcat cggcaaccgg cgcgctgcgc
     5581 gacggggcgc gatgcttgcc cggtttccag cgtcggtgga accagaacca gttggcgggg
     5641 tcgcggcgga cagccgcttc gaatgcgcaa ttgacatcga gcatgatggc ctcgacggac
     5701 cgcgccttgc cgccgaggcg cgtggggatg ggcgggccca gttcgatccg ccattggccg
     5761 aggccggtgc gaaagcagat ggcggtgatc aggaggcagt tgtacctcag ggcgaacacg
     5821 gcgggcgcgg cgcttgtgga acatgtgcgg ccgaagaacg ggacggggag gccgcggttc
     5881 ccggcgtgct gatcggcgag gaagcccacg accagccgtt ggcgattcat ggcctcccgc
     5941 aacgcggcag tctcgaagcg gcgctcgaag tagaggcagc ctgatctggc gcgcagggtt
     6001 tgcaggaggc gattgagcga aggctggcgc agagcgcggt aggtggttgc ggattgataa
     6061 ccgtcgagcc acaggctgcc gcgggcgtag agttcgaaat tgccgaagtg cccgatcgcc
     6121 gcaacgacgc cccgatgccc ctgggcggcg gccaggaccg catccgcgcc tgccacttcg
     6181 aggatggccc ggatttcgtc gtcggacatc gtcgcggtgc gcacggcgca ggcgaatgtt
     6241 tcgccgatcc gacggaagtt ctcgcgggcc agggcgcgaa tcgcccgggg cgattgctcg
     6301 tgtccgaaac agccggtgag gttctcgatg gcggcgcgac ggtg
//
LOCUS       DAOQRO010000193         6338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12280_length_6338_cov_4.079261, whole genome shotgun sequence.
ACCESSION   DAOQRO010000193 DAOQRO010000000
VERSION     DAOQRO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12280_length_6338_cov_4.079261"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKK20_08210"
     CDS             complement(<1..440)
                     /locus_tag="PKK20_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU99909.1"
                     /translation="MSFKVVIVGGVAAGPKVAAKIMRLNPEAEVTVVEKGKFLSYAGC
                     GLPYYISGMVKDQKDLMSTPIGVVRDTAFFQKVKNVRILNETEAIEIDRARKRVRALP
                     KGKKEEIWLDYDKLVLATGAKPVIPPIPNVNLGNIYTLHGMHDA"
     gene            complement(481..1797)
                     /locus_tag="PKK20_08215"
     CDS             complement(481..1797)
                     /locus_tag="PKK20_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU99910.1"
                     /translation="MIRHARQSSKRWVTGTADIRAGEGRAVLAAAVLFFVLLTSLMLL
                     RPVREALGLARGVESVRQLFFVTVLSTLPLVPLFGWLVSRCSRRRLMRVSLHGCAGIL
                     VAFFIGLTLLPESPRLLVAAVYYIFHSVFNLFVISLFWAFMVDHFNLAQSKRLFPAIA
                     LGGSLGAIAGSLISWRLVAYFGVNGLFLLAAALLEAAVWLAEHFTRTHAASLPAPTDM
                     TPIGGSWFAGINAVAQSAYLRGVGVWIVLSGTVSTFLYFTGLRLVGAASVSTAGQTAW
                     FAYINLWTQLATLLAQGLLARRIMRVAGVGGALAVLPLLASGGFALLAAAPALAAFTI
                     VNALFRAAQQGIAGPAEQTLFTVLPQQHKYKAKSFLDTCGYRAGDALGAYLDRFLAIA
                     GFGLLPLAGAVLALSSLWVLLSTFLGRRQARFARPDTSILESEGRL"
     gene            2345..3295
                     /locus_tag="PKK20_08220"
     CDS             2345..3295
                     /locus_tag="PKK20_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU99911.1"
                     /translation="MIAFGPVPSRRLGRSVGINNIPPKLCTYSCVYCQLGRTNAMRMR
                     RRAFYEPENVARDVQTKVERAQRAGETVDYLTFVPDGEPTLDIHLGREIDLLRPLGIK
                     IAVITNASLIGREDVRADLMKADWVSLKVDSTRPEIWRKIDRPHGALRLSSILDGTVE
                     FARIFQGELVTETMLVEGVNDSDDAVGEVAEFLARLKPATAFLSVPIRPPAEKRVRAP
                     REEVINRAYQIFDERMDRVEYLIGYEGNAFAFTGNVEEDLLSITSVHPMRQDAVNEFL
                     MRAGADWAVVDRLLERNELTRTQFGNRQFYLRRLHADSRR"
     gene            complement(3360..3746)
                     /locus_tag="PKK20_08225"
     CDS             complement(3360..3746)
                     /locus_tag="PKK20_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNU99912.1"
                     /translation="MNVAILGASNKPDRYSYKALRMLREKGHTPFPVHPALSMVDGIP
                     VWSSLRAIPEPIDTVTVYLSARNQEAIANDLLDGGMRRVIFNPGAENPELAERLRQHG
                     KEVLEACTLVLLSTGQFAKSGPETAT"
     gene            complement(3835..4740)
                     /locus_tag="PKK20_08230"
     CDS             complement(3835..4740)
                     /locus_tag="PKK20_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU99913.1"
                     /translation="MEEILILSGKGGTGKTSVTASLAALADRPVLADCDVDASSLPLI
                     LKPRLRRQETFIGGLRARIQPGHCVACGKCKELCRFDAIFFDGPSNGQVPRTFRVDPS
                     ACEGCGVCEYFCAERAIELIPAENGRWFVSDTRFGPLIHARLRPGQGNSGKLVTLVRR
                     QARLVAEASGRQLILIDGPPGIGCTTIAALTGTTRMLVVTEPTPSGEHDLERVLELGR
                     HFGVHAWVCLNKHDLNPAMTERIEHKAAALGATIAGRIPYHISVTVAQRQGRPVVELD
                     DSPAAAAIARLWQQIRTSLPTGKVS"
     gene            complement(4725..6197)
                     /locus_tag="PKK20_08235"
     CDS             complement(4725..6197)
                     /locus_tag="PKK20_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU99914.1"
                     /translation="MRLAIASGKGGTGKTTVAVNLAVTLARHGESVAYVDCDVEAPNG
                     HLFLNPTALLESLVDRLVPRIDRKRCTLCGSCARICRFNAILCLPDRVLVYDELCHSC
                     GGCALVCPTQAIAEEPHAIGLLRTGAAQAVQFVSGTLNVGESSSPPVIRAAKQAVPPA
                     EWTILDAPPGTACPMVETVRDCDCVLLVTEPTPFGLHDLRLAVEVARALKLACGVVIN
                     RADPVATETRKWCEQERLPILAEIPDSLAVAKAYSHGQLIIDAVPGMEQTFNQLRERL
                     LAELHNSSAMQRYRKSCETEFWQNVFRAESDYLLAHLTGCQDVLSVGCGPASIEAALN
                     RHGFRVTGLDMSPEAFGGGVEGVRTVVARAQDMPFPPASFDAVIFVASLQFIEDWRKA
                     LAQAARVLRDAGRLVVMLLNPASGFFKEKRRDPHSYVRKMRHSDLNEIEEAVRKRFDV
                     QTEYCLGIKGTTLFESREPASAALYVIRGVRKEPGSWKKS"
BASE COUNT         1270 a         1913 c         2004 g         1151 t
ORIGIN      
        1 tcggcgtcgt gcatgccgtg cagtgtatag atgttgccga ggttcacatt cggtattgga
       61 gggatgaccg gtttcgcgcc agtggcgagg accaacttgt cgtagtccaa ccagatctcc
      121 tccttcttgc ccttcgggag tgcgcggacc cgtttccggg cgcggtcgat ctcgatggcc
      181 tcggtttcgt taaggatccg gacgttcttg accttttgga agaaggcggt gtcgcgcacc
      241 accccgatcg gcgtgctcat caggtccttt tgatccttga ccatgcccga gatgtagtag
      301 ggcaggccgc agcccgcgta ggaaaggaac ttgccctttt cgaccacggt gacttccgcc
      361 tcgggattga gccgcatgat ttttgcggcc accttgggac cggccgccac gccgcccaca
      421 atcacaactt tgaaagacat agatcgcgtg ttttgttcag attcaccgcc aagaagtgtt
      481 ttacaaccgg ccttcagatt caaggatact cgtgtcgggg cgtgcgaaac gcgcctggcg
      541 gcggcccagg aatgtcgaga gcagcaccca gagactcgac agcgcgagca cggcgcccgc
      601 cagcgggaga agaccgaacc cggcgatggc caggaatcga tcgagatagg cgccgagggc
      661 atcgcccgca cggtatccgc acgtgtccag aaacgacttg gctttgtact tgtgttgctg
      721 cggcagaacc gtgaacaggg tttgctcggc tgggcccgca atcccctgtt gggccgcgcg
      781 gaacagggca ttgacgatcg tgaacgcggc gagcgcgggc gccgcggcga gcagagcgaa
      841 gcctccggag gcaaggaggg gcaggaccgc caaagcgccc ccgactcccg cgacgcgcat
      901 gattcgtctg gccagaagcc cctgcgccaa cagcgtggcc agttgcgtcc agaggttgat
      961 gtatgcgaac caggccgtct ggccggcggt cgacacgctg gccgcgccca ccagacgcag
     1021 gccggtgaag tagagaaacg tcgagaccgt gccgctgagg acgatccaca cgccgacccc
     1081 gcgcagatag gcggattgcg cgacggcatt gatcccggca aaccaggacc cgccaatagg
     1141 agtcatgtcg gtgggggcgg gcagcgacgc cgcgtgtgtc cgcgtgaaat gttcggcgag
     1201 ccaaacggcc gcttcgagca atgctgcggc caataggaag aggccgttga cgccgaaata
     1261 cgcgaccagg cgccaggaga tcagcgatcc ggcaatcgcc ccgaggctgc cgcccagggc
     1321 gatggcgggg aagaggcgtt tgctttgggc gaggttgaag tgatcgacca tgaacgccca
     1381 gaagagcgag atgacgaaga gattgaagac cgagtgaaag atgtagtaga cggccgccac
     1441 caacagccgc gggctctcgg gaagaagcgt cagtccgatg aagaatgcga ccagaattcc
     1501 ggcgcagcca tgcagcgaga cccgcatcag cctgcggcgc gagcatctcg agaccagcca
     1561 gccgaagagc gggaccaacg gcaatgtgct gagcacggtc acgaaaaaca gttgccgcac
     1621 actctcgacg ccccgggcca ggccgagggc ctcgcgcacg gggcgcaaga gcatcagact
     1681 ggtaagcagg acgaaaaaca gcaccgcggc ggccagcacg gcgcgccctt cgccagcccg
     1741 aatgtccgcg gtgccggtca cccagcgctt tgacgactgc ctggcgtgcc gaatcatggg
     1801 cgatcggggg gcgcaccctt ccgtccgacg ccgcgcggac tgcgtcggcg ccggaccttc
     1861 acgccaggcc gggccaggca aaacccgcaa gcattcacga agagcatgct gccgaagcta
     1921 cgcaagactc gggcttgccc gcaaacagtt tgtccgacga agctttacaa cgggcagatt
     1981 caacctggcc cgcggtcaca cccaacatga acagatacgt ttcagaatcg gcaattggat
     2041 gtgacacggc ccgcatcatg gctccgcaca ctctaccaat acaacgctgc gtcgcacgac
     2101 ggcgtcaaag gagtcggcat ccggaccgac gattgcgtgt gtcgtagaga acgagctcat
     2161 actttggcac gcggtttgcc atcggttaca ttcgaggttg cgccgatggg gcgtggaacg
     2221 ggatgatcct ggccacgcag caggagcctc aattcgacaa acagaagact atgcccttgt
     2281 tcgaatacca atcgatccca gacacttcat tttcaaccga taagtcgggg aaggaatgga
     2341 cacaatgatt gcttttggcc ccgttccatc gaggcgtttg gggcgcagcg tggggatcaa
     2401 caacattccg cccaagctgt gcacctatag ctgtgtgtat tgtcagttgg gccggaccaa
     2461 cgcgatgcgg atgcggcggc gcgccttcta cgagccggaa aacgtcgcgc gcgatgttca
     2521 gaccaaggtg gaaagggctc aaagagcagg ggagaccgtc gattacctga ccttcgtgcc
     2581 ggatggggaa ccgaccctgg acattcatct cgggcgcgag atcgacctgt tgaggccgtt
     2641 gggaatcaag attgccgtga tcaccaacgc gtccctgatc gggcgcgagg atgtgcgggc
     2701 ggacctgatg aaggccgatt gggtctctct gaaggtggat tccacgcgcc cggagatttg
     2761 gcgcaagatc gatcggcctc acggggccct gcggctttcc tcgatcctcg atgggactgt
     2821 cgagtttgcg cggatattcc aaggtgagtt ggtgaccgag accatgctgg tcgagggggt
     2881 caacgacagc gacgacgcgg tgggcgaggt ggcggagttt ctggctcgct tgaagccggc
     2941 cacagccttt ctgtccgtgc cgatccggcc gccggcggag aagcgcgtga gagccccgcg
     3001 cgaggaagtg atcaatcgcg cctatcagat cttcgatgag cggatggacc gggtcgagta
     3061 cctgatcggg tacgagggca acgccttcgc gttcacgggc aatgtcgagg aagaccttct
     3121 gagcatcacg tcggtgcatc ctatgcgcca ggacgccgtc aacgagttcc tgatgcgggc
     3181 tggagcggat tgggcggtgg tcgatcggct gctcgaacgg aatgaactca cccgaaccca
     3241 attcggcaac cgccaattct acttgagaag actccacgcc gactcgcgcc gatgactcgg
     3301 gatggactgt gctgggagtc tgaccgctcc gtgtcgtaac acagcgtgaa ataggcggat
     3361 tacgtggcgg tttcgggtcc agacttggcg aactgtccgg tgctcagcaa gaccagcgtg
     3421 caggcttcga ggacttcctt tccgtgctgg cgaagccgct cagccagctc gggattctcg
     3481 gcgccgggat tgaagatcac ccggcgcatc ccgccgtcga gcagatcgtt tgcgatcgcc
     3541 tcctggttgc gggcggacaa gtagaccgtc accgtgtcga tcggttctgg aatcgcgcgc
     3601 aacgaagacc agacaggaat gccgtccacc atggacaggg ccggatggac cgggaagggc
     3661 gtatgccctt tttcgcggag catgcgcaat gccttgtaac tgtaacggtc aggtttgttg
     3721 ctggcgccca gtatggctac gttcatcgtt caaatccttt aacctagaaa ctcgctccga
     3781 tcaagccaag ttcccagtcc atcggatgtt tctcccgtgc accgcccatg catttcagga
     3841 aaccttcccg gtgggaaggc tcgtgcgaat ctgctgccat aggcgcgcta tcgccgccgc
     3901 cgccgggcta tcgtcaagtt ccacgaccgg acgtccctgg cgttgggcaa cagtcactga
     3961 gatgtgatag ggaatccgac cggcgatcgt cgcgccaagc gcagccgcct tgtgttcgat
     4021 gcgctcagtc atcgcagggt tcaggtcgtg tttattgagg cagacccagg cgtggactcc
     4081 gaagtgacgg cccaattcaa gcacccgttc caaatcgtgc tcgccggatg gcgttggctc
     4141 tgtgacgacc agcattcggg tcgtgccggt tagggctgcg atcgtcgtgc agccgatacc
     4201 gggcggaccg tcaatcagga tcaactggcg gccggacgct tcggcaacaa ggcgcgcctg
     4261 cctgcgaacg agcgtgacaa gcttacccga attcccctgg cctgggcgca gacgagcgtg
     4321 aatgagcggg ccgaagcggg tgtccgagac aaaccaccgt ccattctcgg cgggaatcag
     4381 ctcgatggcg cgctcggcgc agaaatactc gcagacgccg cagccctcac aggctgacgg
     4441 atcgacacga aacgtgcgcg gcacctggcc gtttgacggt ccatcgaaga agattgcgtc
     4501 gaatcggcac aactccttgc acttgccgca ggcgacacaa tggcccggtt ggattcgagc
     4561 gcgcagcccg ccaatgaacg tttcctggcg tcgcaggcgt ggcttcagaa tcaacggaag
     4621 gctggaggca tccacgtcgc aatcggccag caccggccgg tcggccaagg ccgccagcga
     4681 agcggtcacc gaagttttgc cggtgccgcc tttgccgctt agaatcaaga tttcttccat
     4741 gagccaggtt cctttcggac gccccttatg acgtacaacg ccgcgctggc gggttcccgg
     4801 ctctcgaaca gggtcgtgcc tttgatccca agacaatact ccgtctggac atcgaaccgt
     4861 ttgcggacgg cctcttcgat ttcgttcagg tcagaatgcc gcatcttccg aacataggaa
     4921 tgcggatcgc gccgtttctc cttgaagaag cccgatgcgg gattgagcag catgacaaca
     4981 agccgtccgg cgtcgcgcaa gacccgagcg gcctgcgcca gggcctttcg ccaatcttcg
     5041 atgaattgaa gtgaagccac aaagatcacc gcgtcaaagg acgcaggtgg aaacggcatg
     5101 tcttgggcac gtgccacgac tgtccggaca ccttccaccc cgcccccgaa cgcctcgggc
     5161 gacatgtcca agccggtgac gcggaagccg tgtcggttca gcgcggcttc aatgctggcc
     5221 gggccgcagc ccacgcttaa aacgtcttgg cagcccgtta aatgggctag caggtaatcc
     5281 gattcagcgc gaaagacatt ttgccagaac tccgtttcgc aggatttccg gtatctctgc
     5341 attgcgctcg aattgtgcag ttccgccagc agccgttccc gcaactgatt aaaggtctgt
     5401 tccattcccg gcacggcatc gatgatcaat tgaccgtgcg aatacgcctt ggcgacggcc
     5461 agcgaatcgg gaatctcggc cagaattggc aatcgctctt gttcgcacca cttccgtgtt
     5521 tcagtcgcga ctgggtctgc ccggttaatc accaccccgc aggccagttt caatgcccgt
     5581 gccacctcca cggccagccg taagtcgtgc aagccaaacg gtgtcggttc ggtgaccagc
     5641 aatacgcagt cgcaatcccg cacggtctcg accatgggac acgccgtgcc gggcggggca
     5701 tcgaggatcg tccactccgc cggtggtaca gcttgtttgg cggcccgaat cacaggcgga
     5761 ctgctcgatt cgcccacgtt gagggtgccg gacacgaact gtaccgcctg ggcggcgccg
     5821 gtccggagca ggcctatagc gtgcggctct tcggcgatgg cctgggtggg acacaccaac
     5881 gcgcagccgc cgcaactgtg gcacaactca tcgtagacca ggacccgatc ggggaggcag
     5941 aggatggcgt tgaagcggca gatgcgtgcg cacgagccgc agagcgtgca ccgcttgcgg
     6001 tcgatccgag gaacgagtct gtcgacgaga ctctcgagaa gggccgtcgg gttgagaaaa
     6061 aggtggccgt tcggcgcctc gacatcgcaa tccacgtaag ccactgactc accatggcgc
     6121 gcgagtgtca cggccaggtt gacggccacg gtggtcttgc cggtgccgcc cttgccgctg
     6181 gcaatggcga gcctcatgcg cgtcagcctc gatcggtgcc gacggtctcc ggctgctggt
     6241 cccctttgtt ctccaactga tggaggcgtt gctggaggtt cacaagctca gcttgaagtg
     6301 cttcgagttg ctgccggagt tgttgagctt cggtgttc
//
LOCUS       DAOQRO010000194         6335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12289_length_6335_cov_5.339490, whole genome shotgun sequence.
ACCESSION   DAOQRO010000194 DAOQRO010000000
VERSION     DAOQRO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12289_length_6335_cov_5.339490"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1990
                     /locus_tag="PKK20_08240"
     CDS             179..1990
                     /locus_tag="PKK20_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNU99915.1"
                     /translation="MHQALVTVVEDICGRYGFDRTRMTDIVIAVQQRLGCVSTEAMEI
                     IASKVNSHRVEVESVVTFYAFLSVKPRGRVIIRLCKDIVDWMKGYDEVADAMAKELGV
                     TMGETTADGGITLTSTPCIGMSDQAPAALVNDVVVPNLTPERGRQVIRDLRRHLDPAQ
                     LITMVGDGNNAHPLVHSMVRNNIRRRGPVHFDGFESGCALERTIDQTPENVIATIRAS
                     RLRGRGGAGFATGQKWEFARNASGERRYVLCNADEGEPGTFKDRVLLTECPDQVFEGM
                     TVAGYALGSDSGIVYLRGEYAYLRAFLENVLETRRRQGLLGKAILGKPGFDFDIRIQM
                     GAGAYVCGEETALISSCEGLRGDPKNRPPFPAQKGYMGCPTIVNNVETFSAAARIMER
                     GANWFAEMGSERTTGTKVLSICGDCRKPGVYEVPFGITARELLDMADADNPGAMLIGG
                     PCRQMIGPERFGGQICYDDLATGGAILIFNRARNKLEIAEHYLDFFIEESCGHCTPCR
                     VGLVLLKEVVHKVVSGAGEPSDLDYMRRLANVISQTSRCGLGQAAPNPVLGALQNFHT
                     LYADMVSDRKLGLLSTFDLSKAVADAELRVGRMSQYV"
     gene            2006..2713
                     /locus_tag="PKK20_08245"
     CDS             2006..2713
                     /locus_tag="PKK20_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNU99916.1"
                     /translation="MSNVTFTIDGREVSGRAGQTILKAAEASGIYIPRLCAYKNLVPH
                     GSCRVCSVRVNGRIQAACVQTISDGMVVENDSAEIREFRTAILDMLFVEGNHYCMFCE
                     KSGLCELQALAYRFGITAPQYPFLNPDRPVDLSHPDVYLDHNRCILCGRCVRVSQELD
                     HKNVFQFVDRGYRKRLQVNGEALAATRLAVTDEVTASCPTGALMRKRVGYAVPIGQRP
                     FDAQPIGAGNLQTEDSP"
     gene            2842..3396
                     /locus_tag="PKK20_08250"
     CDS             2842..3396
                     /locus_tag="PKK20_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNU99917.1"
                     /translation="MSTPSKPRVATTSLCGCFGCHMSILDIDERLFALADLVEFARSP
                     INDYKEFDGRCAVGIIEGGCCNEENVHVLEDFRKHCNILVSMGECAINGGIPAMRNNL
                     PLRECLEEAYLKSPGIWNPAGILPNDIEIPLLLHRVYPCHEVVKIDCFIPGCPPPADA
                     IWDTVVALLTGKPVTLAYERLKYD"
     gene            3410..4900
                     /locus_tag="PKK20_08255"
     CDS             3410..4900
                     /locus_tag="PKK20_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNU99918.1"
                     /translation="MTTTTSSARPGPTRKITIHPVTRVEGHGKVTIHLDARNTVTEAR
                     LHIVEFRGFERFILGRPYWEVPVIVQRLCGICPVSHHLAAAKAMDRIVGGEQLTPTAE
                     KMRRLMHYGQIFQSHALHFFHLSSPDLLFGTDGTARSAFGAPPAQRNILAVAARYPDL
                     ARQGVMMRKFGQEVIRVTAGKKIHGTGAIPGGVNKNLSIAERDALLEDVPQMLEWSRR
                     AVGIIRDFTMAHLDELRPFGSFESNHLSLVGQDDGALDLYHGVLRAVDSQGQRIVNDV
                     DYQRYLDVVGEEVRSWSYMKFPFLKSLGPKTGWYRVGPLARLNTCAFIPSPEAEAARQ
                     DFFALTQERPNNITMCYHWARIIELLHAIEVIGGLLEDPDLQGDDLLARGPRREETVG
                     VVEAPRGTLFHHYRVNRNDEVTLCNLIVSTTNNNEPMNRAITRVARDVLGGRPEISEG
                     LLNHIEVAIRAYDPCLSCATHATGQMALSVCLIGPNGELLEQHARH"
     gene            complement(5111..6232)
                     /locus_tag="PKK20_08260"
     CDS             complement(5111..6232)
                     /locus_tag="PKK20_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNU99919.1"
                     /translation="MRLAPPSREPAAVVPAAFERALESVRTKHAPDPHLGLFHVGATR
                     RGSALVLTGEVASASARADALAAVRAAGFEVDDRIAVLPDPALGEATWGLVCLSVVTG
                     RAQPEHTAEVGTQELMGRSVRIWKRSGIWSLVQTADRYISWMEEGSFQRCTASDVEAW
                     NRSALLVVTAFEDRVLDRPDSSAQPVSDVVMGCLVRTIGETGDWYRVELPDHRSGYLP
                     RKAAEDHAAWRQSREASPENVERAARLMLGRPYLWGGSSPRGFDCSGFTKFVFFLNGI
                     GLDRNASHQARQGAAIPLDPSLSTIQKGDLLFFGHRAADGRPERVIHTGIYLGDRLFI
                     HASERVRIGSLDPASPIRDEHRIRTLIQARRVLPSRSHL"
BASE COUNT         1175 a         2044 c         1988 g         1128 t
ORIGIN      
        1 tccaacgctc aacgttcaac gaagcggttc gttaaaagtt ggatgttgaa cgttgggcgt
       61 tcgggtgacc ccttttgttg attatcaacg gcttgcgcag gcggttcatg gagaacactg
      121 ctggaatccg cgggtttatg gtttaccggc ggtgcaggaa cgtctagagt gcatcgcgat
      181 gcaccaagcc cttgtcactg tcgttgagga catttgtggg cgttatggct tcgatcggac
      241 gcggatgacg gacattgtca ttgcggtcca gcagagattg ggatgtgtgt cgacggaggc
      301 gatggagatc atcgccagca aggtgaactc ccatcgcgtc gaggttgaga gcgtggtgac
      361 cttctacgcg ttcctgtccg tcaaaccccg cgggcgagtc atcatccgcc tctgcaagga
      421 catcgtggac tggatgaagg ggtacgacga ggtggccgac gccatggcga aggagttggg
      481 ggtgacgatg ggcgagacca ctgccgacgg cgggatcacc ctgaccagca cgccctgcat
      541 cgggatgagc gaccaggccc cggcggcgct cgtcaacgat gtcgtcgtcc ccaacctgac
      601 acccgaacgc gggcgccagg tcattcgcga cctgcgccgg catctcgacc cggcccagct
      661 catcacaatg gtgggcgatg gcaacaatgc gcacccactc gtccactcga tggtccgcaa
      721 caatatccgc cgcaggggcc cggtgcattt cgacggcttc gagtccgggt gcgcactcga
      781 acgcaccatc gaccaaacgc cggagaacgt gatcgccacc atccgcgcat cgcggctgcg
      841 cggacgcggc ggcgccggat tcgccacggg acagaagtgg gaattcgccc gcaacgcgtc
      901 cggcgaacgc aggtatgtgc tgtgcaatgc cgacgaaggg gagcctggga cgttcaagga
      961 ccgcgtgctg ctgaccgaat gcccggacca ggtcttcgag ggcatgaccg tcgccggcta
     1021 tgcgctgggc agcgattcgg gcatcgtcta cctgcggggg gagtatgcgt atctgcgggc
     1081 cttcctggag aatgtgctcg agacccgtcg ccgtcagggc ctgcttggaa aggcgattct
     1141 cggaaaaccc ggtttcgatt tcgacatccg catccagatg ggggcggggg cctacgtgtg
     1201 cggtgaggaa accgcattga tcagctcgtg cgaaggcctg cgtggcgatc cgaaaaaccg
     1261 accgccgttc ccggcccaaa agggctacat gggctgtccg accatcgtga acaacgtcga
     1321 gaccttcagc gccgccgctc ggatcatgga gagaggcgcc aactggtttg cggagatggg
     1381 atcggagcgc acaaccggca cgaaggtgct ctcgatttgc ggggactgcc gcaaaccggg
     1441 cgtttacgag gtgccctttg gcatcacggc ccgcgaactg ctcgatatgg ccgacgccga
     1501 taatccgggg gccatgctca tcggcgggcc ctgccgccag atgatcggtc cggagcgctt
     1561 cggcgggcag atttgctacg acgacctcgc caccggcggg gccattctca tcttcaaccg
     1621 cgcccggaac aaactcgaga tcgcggagca ttacctggat ttcttcatcg aggagagctg
     1681 cggccattgc accccctgcc gcgtcggcct ggtccttctc aaagaggtcg tccacaaggt
     1741 cgtcagcggg gcgggcgagc cgtccgatct ggattacatg cgccgcctgg ccaacgtcat
     1801 cagccagacg agccgatgcg gcctcggcca ggccgcgccc aatcccgttc tgggcgccct
     1861 ccagaacttc cataccctct atgccgacat ggtgagcgat cgcaaactgg ggctgctctc
     1921 gaccttcgat ttgtcgaagg cggtcgctga cgccgagttg cgggtcggac ggatgtccca
     1981 gtatgtgtga acggagagac caaccatgag caacgtcaca tttaccatcg acggacgcga
     2041 ggtgtcgggc cgggccgggc agaccattct caaggcggcg gaagcgtccg gcatctatat
     2101 cccccgcctg tgcgcctaca agaacctcgt cccgcacggc agttgccgcg tctgctccgt
     2161 ccgcgtcaac ggccgcatcc aggccgcctg tgtgcagacg atctccgacg gcatggtggt
     2221 ggagaacgac agcgcggaga ttcgcgaatt ccgcacggcg atcctcgaca tgctcttcgt
     2281 cgagggcaac cactactgca tgttctgcga gaagagcgga ctctgcgaac tccaggcgct
     2341 ggcctatcgc ttcgggatca ccgcgccgca ataccccttc ctcaatcccg accggcccgt
     2401 cgacctctcg cacccggatg tctacctgga ccacaaccga tgcatcctgt gcggacgatg
     2461 tgtgcgcgtc tcgcaggaac tcgatcacaa gaacgtcttt cagtttgtcg accggggcta
     2521 ccgcaagcgg cttcaggtca acggagaggc gctggccgcc acccgcctgg ccgtgaccga
     2581 cgaggtcacc gcgtcgtgtc cgaccggcgc gctgatgaga aaacgggtcg gctatgccgt
     2641 gccgatcggg cagcggcctt tcgacgcgca gcccatcggc gcggggaatc tccagacaga
     2701 ggactcgcca tgaaccggga tgtgctccta atcgcaatcg taatcctaat cggcgatcga
     2761 ggattgcgat tgcgattacg agtgggaaac tccggctcat ggtccccatg cgcattcaac
     2821 cgttgaatct ccacgccacc catgagcacc ccatccaaac cgcgcgtggc cacgacaagc
     2881 ctttgtggat gtttcggctg ccacatgtcg atcctggaca tcgacgaacg cctcttcgcg
     2941 ctggccgacc tggtcgagtt cgcccgttcg ccgatcaacg attacaagga gttcgacggc
     3001 cgatgcgcgg tggggatcat cgagggcggc tgctgcaacg aggaaaacgt ccacgtcctc
     3061 gaggactttc ggaagcattg caacatcctc gtctcgatgg gcgaatgcgc catcaacggc
     3121 ggcattccgg ccatgcgcaa caaccttccc ctgcgggaat gcctcgaaga agcgtatctg
     3181 aagtctccag gaatctggaa tcccgcgggc attctcccca acgacatcga gattccgttg
     3241 ctgttgcacc gcgtctaccc ctgccacgag gtcgtcaaaa tcgactgttt cattcccggc
     3301 tgtccgcctc ccgccgacgc catctgggac acggtcgtcg ctctcctgac cggcaagccg
     3361 gttacgctgg cctacgagcg gttgaagtac gattgatgaa tcaccacgca tgaccaccac
     3421 gacgtcatcc gcccgcccgg gcccgacccg gaagatcacc atccatccgg tgactcgcgt
     3481 cgagggtcac ggcaaggtga cgatccacct cgacgcccgc aataccgtca ccgaggcgcg
     3541 gctccacatc gtcgagttcc gagggttcga gcggttcatc ctcgggcggc cctactggga
     3601 agtgccggtc atcgtccagc gcctgtgcgg catttgcccg gtgagtcacc acctggcggc
     3661 cgccaaggcc atggaccgga tcgtcggggg cgagcaactg acgccgacgg cggagaagat
     3721 gcggcgcctg atgcactacg gccagatatt ccagagccac gcgctgcact tcttccatct
     3781 ttcgagcccg gatttgctct tcggcaccga cggcaccgcg cgaagcgcgt tcggggcgcc
     3841 gcccgctcag cgcaacatat tggctgtggc cgccaggtac cctgatctgg cgcgccaggg
     3901 ggtcatgatg cgcaagttcg gccaggaggt catccgcgtc accgcgggca agaagattca
     3961 cggcaccggt gcgattccgg gcggggtgaa caagaaccta tcgatcgccg agcgggatgc
     4021 ccttctcgag gatgtgcccc agatgctcga atggagccgg cgcgccgtcg gcatcattcg
     4081 cgatttcacg atggcgcatc tggacgaact gcgtccgttc ggctcgttcg agtccaatca
     4141 cctgtcgctc gtcggccagg acgacggtgc gttggatctg taccacggcg ttctgcgggc
     4201 tgtggactcg caggggcagc gcatcgtcaa cgatgtggat taccagcgct acctcgatgt
     4261 cgtgggcgag gaggtccgct cctggtcata tatgaagttc ccgttcctca aatcgctcgg
     4321 tcccaagacc gggtggtacc gcgtggggcc gctggcgcgg ctcaatactt gcgctttcat
     4381 tccctcgccc gaagccgagg cggcccggca ggatttcttt gccctcacgc aggagcgccc
     4441 gaacaacatc acgatgtgct atcactgggc ccgcatcatc gagctgctcc acgcgatcga
     4501 ggtgattggc ggcctgctcg aggaccctga tcttcaagga gacgatctcc tcgcccgcgg
     4561 tccccgacgc gaggaaaccg tcggggttgt cgaggcgccg cgcggcactc tctttcacca
     4621 ctaccgcgtc aaccgaaacg acgaggtcac tctgtgcaac ctcatcgtct cgaccaccaa
     4681 caacaacgag ccgatgaacc gcgcgatcac ccgggtggcc cgggacgtcc tcggcgggag
     4741 acccgaaatc agcgagggtc ttctcaacca catcgaggtc gccatccgcg cctacgaccc
     4801 ctgcctttcc tgcgccacgc acgccacagg gcaaatggca ctctcggtct gcctcatcgg
     4861 gccgaacggc gagttgctcg aacagcacgc gcggcattga gtgtcccccc gatcatcgcc
     4921 tgagcccgga tgtttcgtga agcttcgcgg tgtctgacat ccgggctccg gcgaatccgc
     4981 gccccggaaa ggaatcccag aacctcgtgt ccattaccag gagagcgact gggattgcct
     5041 tgaataggat gtcaaacgca cgagttgcca taggttttgt gctccggtcg gttccgtgag
     5101 aggtcaggcg ttataagtgc gatctggacg gcagaacacg gcgggcctgg ataagcgtgc
     5161 ggattcgatg ctcgtcgcgg attggggacg cgggatcgag gctgccgatg cgcacgcgtt
     5221 cggacgcatg aatgaacagc cgatcaccca ggtagatgcc ggtgtggatt accctttcgg
     5281 gccggccatc ggcggcgcgg tgtccgaaaa acagcaaatc ccccttctgg atcgtgctca
     5341 ggctcgggtc gagcgggatc gccgcgccct ggcgggcctg gtggctcgcg ttgcggtcca
     5401 ggccgatgcc gttgaggaag aagacgaact tggtgaatcc cgagcagtcg aagcctctgg
     5461 gagagcttcc tccccacagg tacggacggc ccaacatcaa gcgcgcggcc cgttcgacgt
     5521 tctcgggcga ggcctcgcgc gattgcctcc aggcagcgtg gtcctcagcg gcctttctcg
     5581 ggagataccc gctgcgatga tcgggcagtt cgacgcgata ccaatcgccc gtttcgccga
     5641 ttgtccgcac cagacacccc attaccacat cggacacggg ctgcgcgctc gaatccggac
     5701 gatcgaggac gcggtcctcg aaggccgtga ccacgagcag ggccgaacga ttccaggcct
     5761 cgacgtccga ggcggtgcag cgctggaacg agccttcctc catccaggag atgtagcggt
     5821 cggcggtctg gaccagagac cagatgcccg agcgcttcca gatgcgaacg gaacgtccca
     5881 tcaattcctg tgtgcccacc tcggccgtat gctccggttg cgcgcggcca gtgacaacgc
     5941 tcaggcagac aagcccccac gtggcctcac ccaacgccgg gtccggcaac acggcaatcc
     6001 ggtcgtcgac ttcaaacccg gcagcccgga cggcggcgag ggcatccgcc ctcgcagagg
     6061 cgctcgcgac ctcgcccgtc aacaccagtg cgctgccccg acgcgttgcg ccaacgtgaa
     6121 agagaccaag gtgaggatcg ggggcatgct ttgtgcggac cgattcgagc gcgcgttcga
     6181 aggcggcggg gaccaccgcg gccggctccc gggagggagg cgcgagtctc acgccggaag
     6241 gtgtttctga aagcgtttgg ggcgacgagg ccctgcctac cctgcctgcc ggcaggcagg
     6301 cgtcgccgct ccgcaccggt tcatggtcat cgtgt
//
LOCUS       DAOQRO010000195         6332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12307_length_6332_cov_5.807392, whole genome shotgun sequence.
ACCESSION   DAOQRO010000195 DAOQRO010000000
VERSION     DAOQRO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12307_length_6332_cov_5.807392"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1250
                     /locus_tag="PKK20_08265"
     CDS             333..1250
                     /locus_tag="PKK20_08265"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNU99920.1"
                     /translation="MNGPTSKLTEDLIASYAHIGGINHLDGENLPSKRAIALITVDLL
                     RLLFPGYFDERIVHSSEVRTQTDALIGSVQRRLATELRKSLEYAPPADRELGDVGRVA
                     SELTVEFLRCLPRVREVLQTDVEAAYQGDPAALSEEEVIVAYPFIEAIAVQRLAHELY
                     RHRVPLIPRIMTEWAHARTGLDLHPGARIGSHFFVDHCTGTVVGETSEIGRNVKLYQG
                     VGLVARSLTGGQALRGQKRHPTIEDRVTVYAGATIMGGDTVVGAGSTIGANVFLTQSV
                     PPGSLVLSEDVRIRVLAKSDLAPGGDYQI"
     gene            1254..2726
                     /locus_tag="PKK20_08270"
     CDS             1254..2726
                     /locus_tag="PKK20_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNU99921.1"
                     /translation="MPVPVVTISQMREWEQATWAAGQTPDAVIRRVGECLARRALSLT
                     REGDLILILAGRGHNGDDARCAEPHLGERRVEVLSIRDPAASRTALVAALGKGPGLVI
                     DGLFGIGLNRPLDSAWIEFIAALNAARVRVLSVDVPSGIDADTGEALGAAVESTVTLT
                     LGAPKVGLLRSGAWAAVGRLELESDIGLVPCPCESDLQWMSGSDFAGYPPARLAAAHK
                     GSFGHLAIVAGSLGYHGAAVLAARGAQRARPGLVSLYTAPSVHAPVASQLQAVMVHPW
                     RSDVRWPDGVSAFLCGPGLADRNLPGDLRHAVIELWQDADEPVIVDASALDWLPAKPT
                     ASRAVRVVTPHPGEAARMLGSSVADVQRDRTAALRALSRQYGGCWVVLKGHQTLVGRD
                     RGPVCVNSTGNPGLAQGGSGDVLAGYLAGLLAQPALEGDPALAARYAVWQHGAAADRL
                     SEGHPNWTIEDLAAHLGGCAESGEASGWWGRLSCGSGKKR"
     gene            3168..3590
                     /locus_tag="PKK20_08275"
     CDS             3168..3590
                     /locus_tag="PKK20_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNU99922.1"
                     /translation="MALNKISATFQFAKRPQLHTEATSEAAWTPNTDVYLTDGGLVIK
                     VELAGMRREDLELTVEGNRLRICGFRRDACREGRCKFVLMEINYGPFESVIELPPGYD
                     ASSAKAAYQNGFLRVDVPLSSPAAAQTFSVPIDTDDGF"
     gene            3652..6102
                     /gene="lon"
                     /locus_tag="PKK20_08280"
     CDS             3652..6102
                     /gene="lon"
                     /locus_tag="PKK20_08280"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNU99923.1"
                     /translation="MHSADTESISISNQTGTSADLPNPRVAGRALPDVLPILGLSDIV
                     VFPGMVVPLVVESSPSTRLIDDVVAGDRFVGLVLQKKAELENPGPDDLWTAGCAARVL
                     RMLKFPDNTTRVLVEGLRRIRLIEFVSQDPYLQARHEHVPDVTDDSIETTALARSARE
                     HFVEVIEHSPTLNEQIKIPALQTEQPGRLADLMAAHLNLTLEERQTLLETADVKQRLK
                     RILPLLTRELEVLKLGSKIQKEVTTSMSKSQRDYYLREQIRAIQRELGEGEEENTEAA
                     ALREQIEAAQLPPEAHKVALKELDRLRQIPPAAAEYTVSRNYLDWIVNLPWARTTPDK
                     LDLAEAERILDTQHYGLIKVKDRLIEFLAVLKLRQQLKGPILCLVGPPGVGKTSLGKS
                     VADALGRKFVRIALGGMRDEAEIRGHRRTYIGALPGRILQSLRRVESRNPVMLLDEID
                     KVGADFRGDPAAALLEVLDPEQNQAFVDHYLDLPFDLSRVLFITTANWLDPVHPALRD
                     RLEVIELPSYTAEEKYHIARRHLLPRQLAEHGLRRRDIHLPPATLRRLIRDYTREAGV
                     RQLDRELAALSRKAARRLARRGGKTKPLSVRPDALSDYLGPVKFLAERAETIRDCGIA
                     TGLAWTPVGGEILFVEATRMPGTGKLTLTGSLGEVMKESAMAALSYLRSLASSPDTPG
                     LKFDKHDLHIHVPAGATPKDGPSAGLTMVVALASLLHQRRVRSDTAMTGEISLRGRVL
                     PVGGIKEKVLAAARSGIRRVILPRGNQKDWREIPKEVSRRLAVEFVDRIDQALEAALA
                     TGHPRKSVNRRKARRTLP"
BASE COUNT         1093 a         1932 c         2199 g         1108 t
ORIGIN      
        1 acccgtaacc tctgccgtga cagggcagcg ctctaaccaa ttgagctacg cccccgcatt
       61 gggaccggca ctatagaaga gaaccaccgc ggtcgtcaac gcggatttcg agtctgacga
      121 atgcgcacgc atctgacagt tgtcggtatt ctgtcgcccg gtgagggcac cgggctagaa
      181 gggatggagc gtcccgctgc aggccgcgtg ccctgcctgc gccgcaaagg gcagcggcct
      241 gtctgccgtg tcgggcacgg cacaagcagg caagcaggcg cggcagggtg gggcggcttg
      301 cgttcattcc tcgagtgctg gataatcagg cggtgaacgg gccgacttcc aagttaaccg
      361 aggacctgat cgcatcgtac gcgcacattg gcggcatcaa ccatttggac ggcgagaacc
      421 tgccgtcgaa acgggcgatt gcactgatca cggtcgatct gttgcgtctg ctgtttccgg
      481 gctacttcga cgagcggatt gtccactcgt cggaggtgcg gacgcagacg gacgcactga
      541 taggatcggt gcagcggcgg ctggcgaccg agttgcgcaa gagcctcgaa tacgcaccgc
      601 ccgcggaccg tgagctcggg gatgtggggc gtgtggcatc ggaactgacg gtcgagttcc
      661 tccggtgcct gccgcgggtg cgcgaggtgc tccagacgga tgtcgaggcg gcttatcagg
      721 gcgatccggc cgcgctgagc gaggaagagg tcatcgtggc gtacccgttt atcgaggcga
      781 tcgcagtcca acggctcgcc catgagttgt atcggcaccg ggtgcccctg atcccgcgca
      841 tcatgaccga gtgggcgcac gcccgcactg ggttggattt gcacccgggc gcgcggattg
      901 gcagtcactt ttttgtggac cattgcaccg gcacggtcgt gggcgagacc tcggagatcg
      961 ggcgcaacgt gaagttgtat cagggcgtcg gcctggtggc ccgttcgctc acgggcggcc
     1021 aggctttgcg gggacagaaa cggcacccga ccatcgagga tcgggtgacc gtatacgccg
     1081 gggcgaccat catggggggc gacacggtgg tgggggccgg cagcaccatc ggcgccaacg
     1141 tcttcctcac ccaaagcgtg ccgcccggct cgcttgtgct cagcgaagac gtccgaatcc
     1201 gcgtcctcgc gaagtcggac ctggcgcccg gaggagatta tcagatctga attatgccag
     1261 tccccgtcgt cacgatcagc cagatgcggg aatgggaaca ggcgacgtgg gccgcgggac
     1321 agacccccga cgccgtgatc cggcgcgtcg gcgaatgcct ggcccggcgc gccctgagcc
     1381 tgacccggga gggcgacttg atcctgatcc tggccggacg cgggcacaac ggcgacgacg
     1441 cgcgatgcgc cgagccgcac ctgggcgagc ggcgcgtcga ggttctgtcg attcgggacc
     1501 ccgccgccag ccggaccgca ttggtcgccg cgttgggcaa aggccccggg ctggtcatcg
     1561 atggtctgtt cggcatcgga ttgaaccgcc cgctggattc ggcgtggatc gagttcattg
     1621 cggcgctcaa cgcggcgcgg gtgcgggtgc tgtccgtcga tgtgccgtcc gggatcgatg
     1681 ccgacacagg ggaagcgctc ggggctgcgg tggagtctac ggtgacgctc accctggggg
     1741 cgcccaaggt cggcttgctt cgttccgggg catgggcggc ggtcgggcgg ctcgaactcg
     1801 aatccgacat cggcctggtc ccgtgtccgt gcgaatcgga tttgcagtgg atgtccggaa
     1861 gcgactttgc cggttatcct ccagcccggc ttgctgccgc tcacaagggg agcttcggac
     1921 accttgcgat cgtggcaggc agcctcgggt atcacggcgc tgccgtgctg gcggcgcgcg
     1981 gggcgcaacg ggctcgtccg ggcttggtat cgctctacac ggctccgtcc gtgcacgcgc
     2041 cggtggcgag ccagttgcag gctgtcatgg tccacccgtg gcgctccgac gtccgatggc
     2101 ccgatggggt gagcgccttt ttgtgcggac cggggttggc ggatcgaaat ctgccgggcg
     2161 acctgcgtca tgccgtgatc gagctgtggc aggatgccga cgagccggtg attgtggacg
     2221 ccagcgcgct ggattggctc ccggcgaagc ccacggccag ccgggcggtg cgcgtcgtca
     2281 cgccgcatcc gggggaagcg gcccggatgc tcggttcgag cgtggcggat gtgcagaggg
     2341 atcggacggc agccctgcgt gcgctatccc ggcagtacgg cgggtgttgg gtggtattga
     2401 aggggcacca gacgcttgtc ggacgcgacc gcggacccgt ctgtgtgaac tcgacaggga
     2461 atcccggtct ggctcaaggc gggagcggcg acgtgctggc gggctacctg gcgggcttgc
     2521 tggcgcaacc ggcgctcgaa ggcgatccgg ctttggcggc gcgttacgcc gtctggcagc
     2581 acggcgcggc ggcggatcgg ttgtcggaag gccacccgaa ctggacgatc gaggatctgg
     2641 ccgcgcacct gggcggttgc gctgagagcg gggaggcgtc tgggtggtgg gggcgtctga
     2701 gttgcggcag cgggaaaaag cggtaagctg cgcgcatgga tggacagcag attgcggcgt
     2761 tggggatcgt tgtgctgacc gcgggcctga tggtgagggg ctggctgagg cggcggacgc
     2821 ggcgcggcgt ggacgtgggc gtctgtgtga agtgcgcctg tgcggcgggt cggggtatcg
     2881 agcgacggaa cagtgcatct gctggttctc ggtgagaatg gagtctgcgc cgcgtggggg
     2941 cctgcctgcc gcgcctgcct gcgccgtgcc cgacacggca gacaggccgc tgcgctttgc
     3001 ggcgcaggga gggcatgcgg tgcacagtat gagtctcgat ccgttgcagg cccggcgtcc
     3061 tcaccgggcg acagtatacc gacaactgtc cgatgtaccg gcgacggaag cggacgcaag
     3121 cggaggcttg aaaggcgggc ggaggtgcat tagcttcacc ggcatcaatg gccttgaaca
     3181 aaatttcggc aacatttcag tttgccaaac ggcctcagct tcacacggaa gcgacatccg
     3241 aggcggcatg gactccgaat acggacgtct acctaacgga cggcgggttg gtgatcaaag
     3301 tcgagctggc ggggatgaga cgggaagatc tcgaactgac ggtcgagggc aaccggctgc
     3361 ggatttgcgg tttccggcgg gacgcgtgtc gggagggccg gtgcaagttt gtgctgatgg
     3421 agatcaacta cggaccgttc gagagcgtga tcgagctgcc tcccggctat gacgcgagca
     3481 gcgccaaggc ggcctatcag aacggcttct tgcgggtgga cgttcccctg tcctcgcccg
     3541 ctgccgccca gacgttcagc gtcccgatcg acaccgacga cggtttttga cttttccgtg
     3601 aagcttcttc gaggcgggtg catctaatcg cctgtctaga ctgaactggg gatgcactcc
     3661 gccgacaccg aatcgatcag tatctcgaat cagaccggga cttccgcgga cttgcccaac
     3721 ccgcgcgtcg cggggcgggc attgcccgac gttctgccga ttctcgggct ttcggacatc
     3781 gtggtgttcc cgggcatggt ggttccgctg gtcgtcgagt cgtccccgag cacgcgcctg
     3841 attgacgatg tggtggcggg ggaccggttt gtcggactgg tgctccagaa aaaggcggaa
     3901 ctcgagaacc cgggtccgga cgatctgtgg actgcgggct gcgccgcgcg cgtgctccgc
     3961 atgttgaagt ttccggacaa cacgacgcgt gtgctggtcg aaggccttcg ccgcatccgt
     4021 ctcatcgagt ttgtttccca ggacccgtac ctgcaagcgc gccacgagca tgtgccggac
     4081 gtgacggacg actcgatcga gacaacggcc ctggcgcgga gcgcgcgcga gcattttgtg
     4141 gaggtcatcg agcactcgcc gacactcaac gagcagatca agattcccgc tctacagacc
     4201 gagcaaccgg ggcgtctggc ggatttgatg gcggcccacc tgaatctcac gctcgaagaa
     4261 cgccagacat tgctcgagac ggcggatgtc aaacaacggc tcaagcggat tctgcccctg
     4321 ctcacacgcg agctcgaggt tttgaaactg ggctcgaaga tccaaaaaga ggtcacaacc
     4381 tcgatgtcga agagccagcg cgattattac ctgcgcgagc agatccgggc gattcagcgc
     4441 gaactgggcg aaggcgaaga ggaaaacacg gaagccgccg ccttgcgcga acagatcgag
     4501 gccgcccaat tgccgcccga ggctcacaag gtcgcgctga aggaactcga tcggctccgc
     4561 cagattccgc ccgctgcggc cgagtacacg gtctcgcgca actacctcga ttggatcgtg
     4621 aacctgccgt gggccaggac aacgccggac aagctcgacc tggcggaagc ggagcgcatt
     4681 ctcgatacgc agcactacgg cctgatcaaa gtcaaggatc ggctgatcga gttcctggcc
     4741 gtgctcaagc tgcggcagca actcaaagga cccatcctct gcctggtcgg cccccccggc
     4801 gtgggcaaaa cctccctggg caagagcgtc gcggatgccc tcggacgcaa gttcgtgcgg
     4861 atcgccctcg ggggaatgcg tgacgaggcc gagattcgcg gtcatcgccg cacctacatc
     4921 ggcgccctgc cgggtcggat tctgcagagc ctgcgccgtg tcgagagccg caacccggtc
     4981 atgctgctcg acgagatcga caaggtcggc gccgatttcc gcggcgatcc cgcggccgcg
     5041 cttcttgaag tgctcgatcc ggagcagaat caggcgtttg tcgatcatta cctcgatttg
     5101 cccttcgatc tctcccgcgt tctgttcatc acgacggcca actggctcga tcccgttcac
     5161 cccgccttgc gggatcggct cgaagtcatc gaactgccca gttacacggc cgaggagaag
     5221 taccacattg cgcgccgcca cctgttgcct cgtcaactgg ccgaacacgg cctgcgccgg
     5281 cgtgacatcc acctgccgcc cgccacgctg cgccgcctca tccgcgatta cacccgcgag
     5341 gccggcgtac gccagttgga ccgcgaactc gcggccctgt cccggaaggc cgcccgccgc
     5401 ctggcgcggc gcgggggaaa gaccaagcca ctctcggtgc gtcccgacgc gttgtcggat
     5461 tacctggggc cagtgaagtt tttggccgag agggccgaga cgattcgcga ttgcgggatt
     5521 gcgacaggtc tggcctggac gcctgtgggt ggcgagattc tcttcgtcga agcgacacgc
     5581 atgccgggga ccggcaaact gactctgacc ggctcgttgg gcgaggtgat gaaggagagt
     5641 gccatggcgg ctttgagcta cttgcgcagc ctggcctcga gcccggacac tcccggtctc
     5701 aagttcgaca agcacgactt gcacatccat gtccccgccg gcgccacacc caaggacggg
     5761 cccagcgcgg gtctgacgat ggtggtggcc ctggcctcgc tcctgcacca gcggcgggtg
     5821 cgatccgata cggcgatgac cggcgagatc agcctgcgcg gacgggtcct gccggtggga
     5881 ggcatcaagg agaaggtgct tgcagcggcc cgatcgggta tccgacgtgt cattctgccc
     5941 cgaggaaacc aaaaagactg gcgtgaaatc cccaaagagg tgagtcggcg gctggcggtc
     6001 gagtttgtgg atcggattga tcaagccctc gaggccgccc tggccaccgg gcatccgcgc
     6061 aaatcagtga atcggagaaa agcgaggcga accttgccat gaaccgaacc ttggacggga
     6121 ggacgcctgc ggcgccaggt tcatgttcat tgggccgctc cgccaggaac atgtagcttt
     6181 ccatgaaccg tggttccgta gccgcggacg tgagtccgcg ccatctcgct ccgtcaacag
     6241 cagaaatcag cgccgactca cgtcggcggc tacaaatcgg ttcatgggtc caatgcgcat
     6301 taaaccgttg aatgtcaatg ctttccatga ac
//
LOCUS       DAOQRO010000196         6330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12312_length_6330_cov_5.395378, whole genome shotgun sequence.
ACCESSION   DAOQRO010000196 DAOQRO010000000
VERSION     DAOQRO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12312_length_6330_cov_5.395378"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PKK20_08285"
     CDS             complement(<1..1218)
                     /locus_tag="PKK20_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU99924.1"
                     /translation="MANIKAKKRRKLVVLLLIVVALGGLGALAIFKKREAAITVQTEK
                     VERRTLTELVVANGRIQPVFQVKISPEVSGEIIELPVKEGDQVKKGDLLLKIKPDLYV
                     AQRNSSEANYKGALAGRVTAEANLKKAEIDLQRAEALHESRLLPDAEFLAVQTTCEIN
                     KAQLQHAIHQVEMAKAALEKAEEDLSKTTIVSPLDGTVSRLNSQLGERVVGTAMMAGT
                     EVMVIADLDEMEARVDIGEIDVVLIQLGQNARLEVDAYKDRKFNGTVTEIANSSKGLV
                     FGSGGGSQSTDATKFEVKIRIKEKEPFRPGMTVTAEVETRSRTNVLSVPIASVTTRIP
                     KPPGQNKTPAKGAQAATAAKDPAAAANSTAQTGTNTPAPETAQATNAAPGTNDTATTA
                     SKKIVALRAFGPAR"
     gene            complement(1369..2163)
                     /locus_tag="PKK20_08290"
     CDS             complement(1369..2163)
                     /locus_tag="PKK20_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNU99925.1"
                     /translation="MNDSAPLDSPDDAPPIPDSTSLSARMLNVFATPADVFDEVKVSP
                     PRTANWLVPTLLATLVGWIGAWLVLQQEAITQQISDLQEAQYQKMIEQGRMTREQLEA
                     QRPTIEKFGKIMRQAAMFAAPPLAAVGLVFWWALITFLVGRYALGGEFTYMKAVEVSG
                     LSGMLAILESVIKTLMMVAFGSVYAGPNLAMLLVKDFDPANLSHGLMAAVDVVAFWVL
                     AVRAIGLSRLSGATFAKSAAWVFGLWLLWTGLWIVAGHFLPRLFGG"
     gene            2733..3851
                     /locus_tag="PKK20_08295"
     CDS             2733..3851
                     /locus_tag="PKK20_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNU99926.1"
                     /translation="MLCALVAVLIFSRASFGGEGQPAFIPEASRGEAIAPSKNPPFYS
                     DKLNLLCFRDEAGVEHPVLGTADWMKRRGHILAAMEQVMGVLPPLEFNGPPQVEVLEE
                     TPTANGVRRKILYTVESPTNRVPAYLFIPRSGGAKRPAMLCLHQTTPEGKAEMAGLRG
                     RTNRQFGLEVAQRGYVTLVPDYPNFGDYRRDAYAAGYASATMQGIVNHRRALDLLASM
                     PEVDARRIGVLGHSLGGHNALFLAVFDERARVVVTSCGFNAFSKYYGGNLAGWSHAGY
                     MPRIRSVYGCDPGRMPFDFTEVLAAIAPRPVFINAPLHDDNFEVSGVKDCVQAAAPVY
                     RLLGAVEALVAVYPDAEHDFPPEVRRQAYAWLDRQLGL"
     gene            complement(4015..5007)
                     /locus_tag="PKK20_08300"
     CDS             complement(4015..5007)
                     /locus_tag="PKK20_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNU99927.1"
                     /translation="MSAQTAITPIAPAPPPEPDESPDPHVAQFLEHLAVDRAASPYTR
                     RNYQHALREFGHWHRLERGAPPAWPSLERDDFRGYLRFLGRGQLSRAAVALRFSALRS
                     FYRFLVRRGVVLTTPIRSLSLPKLPRRLPRFLTADHAAALLRAPLDELAQLEQAGDPP
                     TGSDEFHRDAALLETIYSCGLRISEACGLRAEDVAWNDQLIRVRGKGRKERQIPIGAP
                     ALEAIRAYWRLLPAPPAGSLPVFLAQANSTRPVSARVVQMRLKRYLARAGLDPHLTPH
                     KLRHSFATHLLDAGADLRSVQELLGHAHLATTQIYTHLTTERLKRVYDQAHPRA"
     gene            complement(5004..6158)
                     /gene="holA"
                     /locus_tag="PKK20_08305"
     CDS             complement(5004..6158)
                     /gene="holA"
                     /locus_tag="PKK20_08305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNU99928.1"
                     /translation="MRKPSAPLVLVCGEDDFAVQQRGRQLYADWCKEIGGMDHETIDA
                     TVSNSQEALRSLARLREALQTLPFFGSGKVIWLRQCNFLGDERVAGAQAVTVSLNDLA
                     QELKRFPWDNVRLLITAGKVDRRKTFSKTIEKLGAVEYLAGLSADDKDWADRAERFVR
                     GELRTRRQEMSDEALGELVSAVGPNLRQLSQEIEKLSLYAAGRPEITVADVHAVVSRN
                     KQARAFALGEALGDRDLPRALRCLDEELWEMQFDRQKTAIGLLYGLISKVRTLLLLKE
                     MFRLGLLAPDVDYNRFKSQLGRLPSEAMPDDKRYNPAAMHPYVLFKALPQAGQYTAEE
                     LVRAMDVLLVSNRRLVSSQLEEALVLQQALVQIVGRPPGRRPGRLTARAS"
BASE COUNT         1064 a         1997 c         2089 g         1180 t
ORIGIN      
        1 gcgcgcaggg ccaaaggccc ggagagccac gatcttcttc gacgcggtcg tggcggtgtc
       61 gtttgttccg ggggcggcat ttgtcgcctg ggcggtctcc ggggcgggtg tgttggttcc
      121 agtctgggcg gtgctgttgg cggccgctgc ggggtccttg gcggcggttg ccgcttgcgc
      181 gcccttggcg ggcgtcttgt tttggcctgg aggtttcggg atgcgcgttg tcacgctggc
      241 gatcggaacg ctcagcacat tggtgcgcga gcgtgtctcg acctccgccg tcaccgtcat
      301 ccccggccgg aacggttcct tctccttgat gcggattttg acctcgaact tggtggcgtc
      361 ggtcgattgg ctgccgccgc cgctgccgaa caccagcccc ttcgacgagt tggcgatctc
      421 ggtcaccgtc ccgttgaact tgcggtcctt gtaggcgtcc acctcgagcc gcgcgttctg
      481 gcccaactga atcaggacca catcgatctc gccgatgtcg acgcgcgcct ccatctcgtc
      541 gaggtcggcg atcaccatga cctcggtccc ggccatcatc gcggtgccga ccacccgttc
      601 gcccagttgc gagttgagcc ggctcacggt gccatcgagc ggcgacacga tcgttgtctt
      661 ggacaggtct tcctccgcct tctcgagcgc ggccttggcc atctcgacct gatggatcgc
      721 atgttggagt tgggccttgt tgatctcgca cgtggtctga acggcgagga attcggcatc
      781 cggcagcagc ctgctctcat gcagcgcctc ggcccgttgc agatcgatct ccgccttctt
      841 caagttcgcc tcggccgtga ccctgcccgc caatgccccc ttgtagtttg cctcggacga
      901 gttgcgctgc gcgacgtaca ggtccggctt gatcttcagc agcagatcgc ccttcttgac
      961 ctggtccccc tccttcaccg gcagttcgat gatctcgccg ctcacttccg ggctgatctt
     1021 gacctggaac accggttgaa tccgcccgtt cgccaccacc aactccgtga gtgtccgcct
     1081 ctcgaccttt tcggtctgca cggtgatcgc ggcctcgcgt ttcttgaaga tcgcgagggc
     1141 gcccaagccg cccagagcca cgacaatcaa gagcaggaca accagcttcc gcctcttctt
     1201 cgccttaatg ttcgccatct cggtatatcg ttaagacaca acacccgtca gtttgtttcg
     1261 gcgcgtccag accggacatt tcccaagaaa catgctttcc tagcctgcgt gctgacttct
     1321 ctcgagaact ccggcttcgc ggcggcaaaa agtcgtgaga aatgcgggct agccgccgaa
     1381 cagccgcggc agaaagtgcc cggcgacgat ccaaaggcca gtccagagca gccacagccc
     1441 gaacacccag gcggcgctct tggcgaacgt ggcgccgctc aatcgcgaca ggccgatcgc
     1501 gcgcacggcg agcacccaga acgccacgac atccacggcg gccatcaaac cgtgcgagag
     1561 attggccgga tcgaaatcct tgaccaacag catcgccaag ttgggcccgg cgtacacgct
     1621 cccaaaagcc accatcatga gcgtcttgat caccgattcg agtatcgcca acatgccgct
     1681 cagcccggac acctcgacgg ccttcatgta cgtgaactcg ccgcccagcg cgtacctgcc
     1741 caccagaaac gtgatgagcg cccaccagaa gacgagtccg acggccgcca ggggcggcgc
     1801 ggcgaacatc gccgcctgcc gcatgatctt cccgaacttc tcgatcgtcg gtctctgggc
     1861 ttcgagttgc tcgcgggtca tccggccttg ttcgatcatc ttctggtatt gcgcctcctg
     1921 caggtcgctg atctgctgcg tgatcgcctc ctgctgaagc accagccacg ccccaatcca
     1981 gccgaccagc gtcgccagca gcgtcggcac cagccagttg gctgttcgcg gcggactgac
     2041 cttcacctcg tcgaacacgt cggcgggcgt ggcaaagaca ttgagcatcc gcgcgctcag
     2101 cgacgtcgag tcggggatcg ggggtgcgtc gtccggcgaa tcgaggggag cactgtcgtt
     2161 cataacggca aaggtgtaaa agtatactgc gtttggcgtc ggcgacaatc tccgaatgcg
     2221 cgcggttgtc agctacaact cgacgcgggg tttgtacttc acacccggag ttcgcgtccg
     2281 ccgcttccaa cgcggtcgca ctcagtgcat ctgtcagttg tcggtgggaa tggattttac
     2341 gccgcgttgg gcctgcctgc cgcgtctgcc tgtgccgtgc ccgacacggc agacaggccg
     2401 ctgcgctttg cggcgcaggc agggcacgcg gcctacagcg ggaattacga tccgttgcag
     2461 gcccggcgcc ctcgccgggc gagagaatcc cgacaaccgt cagatgcacc ggatcacact
     2521 gaagggcgcg caacggggat gacaggacaa gagaaacacg gtacaaaaca gtccaagaaa
     2581 cacttgtgca aatgaccccg aatacaacac gcatgaggcg ccgaccggtt cgcagcgcga
     2641 gccttgtcgc gaaagtccaa gaggggcgcg gcgctcgcgc ctcttcggcg gtcggatttt
     2701 ctctgtgcga tccgcggagg ctctgtctgg cgttgctgtg tgcgcttgtg gcggtgttga
     2761 ttttcagtcg agcgagtttc ggaggagaag gccaacccgc attcatcccc gaggcctctc
     2821 ggggtgaagc catcgcgccc tcgaagaatc caccattcta ctcggacaag ctgaatctgt
     2881 tgtgctttcg ggatgaggcg ggcgtcgagc accctgtgct tgggacggcg gattggatga
     2941 agcggcgggg ccatattctt gccgcgatgg agcaggtgat gggggttcta ccgccgctcg
     3001 aattcaatgg tccgccccag gtcgaggtgc tcgaggaaac cccgaccgcc aacggcgtgc
     3061 gccggaagat tctgtacacc gtggaatcgc ccacgaaccg ggtgccggcc tatctgttca
     3121 tcccgcggag cggcggcgcc aaacgtcctg cgatgctctg tctgcaccag acaacgccgg
     3181 agggcaaagc cgagatggcc ggattgcgcg gcaggacgaa tcggcagttt ggtctcgagg
     3241 tggcgcaacg aggctacgtg acgctggtgc cggactaccc gaatttcggc gattaccggc
     3301 gcgacgccta tgcggccggc tacgccagcg ccaccatgca gggcatcgtc aatcaccggc
     3361 gcgctctcga tctgctggcg tcgatgcccg aagtggacgc ccgccgcatc ggcgtgctcg
     3421 gtcattcgtt gggggggcat aacgcgctgt ttctggccgt gttcgacgag cgcgcgcggg
     3481 tggtggtgac cagttgcggc ttcaacgcgt tctcgaagta ttatggcggc aatctggccg
     3541 gttggagcca tgcagggtac atgccgcgga ttcgttcggt gtacggctgc gatccagggc
     3601 gaatgccatt tgatttcacc gaggtgctcg ccgccatcgc cccgcggccc gtcttcatca
     3661 acgcgccgct gcatgatgac aatttcgaag tgtcgggcgt caaggactgc gtccaggccg
     3721 ccgccccggt ctaccggctg ctgggcgcgg tcgaggcgct ggtggccgtc tacccagatg
     3781 cggagcacga ctttccgccc gaggtgcgcc ggcaggctta cgcctggctc gaccggcaac
     3841 tggggctttg acgttgcatc tggatgcgcg gcccgtccgg tcacgtgcaa agtcgcggtc
     3901 aacgtcgacg tggaaggatg acatgtggac gaagaccatg aagcggaacc gttagccgtt
     3961 caccgttcaa cgctcaaccc atcaaccatc aaccatctgc gatcagccat cggatcacgc
     4021 ccgggggtgg gcctggtcgt acacccgctt caggcgctcc gtcgtgaggt gggtgtaaat
     4081 ctgggtggtg gcaagatggg catgaccgag cagttcctga acgctgcgca aatccgcccc
     4141 ggcatcgagc agatgggtag cgaaactgtg ccgcagcttg tgcggcgtga ggtgcgggtc
     4201 cagcccggca cgcgcaagat agcgcttgag gcgcatctga acgacgcgcg ccgagaccgg
     4261 acgcgtgctg ttggcttgag cgagaaaaac aggcagggat ccggcgggcg gcgccgggag
     4321 caggcgccaa tacgcgcgga tcgcttcgag cgccggagcg ccgatgggaa tctgccgttc
     4381 tttgcgtcct ttgccgcgga cgcggatgag ttgatcgttc caggcgacgt cctcagcccg
     4441 caagccgcac gcctcgctga tgcgcagccc gcacgagtag attgtctcga ggagggcggc
     4501 gtcgcgatgg aactcgtccg atcccgtcgg cggatcgcct gcctgttcga gttgggccag
     4561 ttcgtcgaga ggcgctcgga gcagagctgc cgcatggtcg gcagtcagga accgcggcag
     4621 acggcgcggc aatttcggca gagagagact gcgaatgggc gtggtgagga ccacgccgcg
     4681 ccggacgagg aaccgataga aggagcgcag ggcgctgaag cgcagcgcaa cggctgcgcg
     4741 gctcagttgg ccgcgtccga gaaagcgcag gtagccgcga aaatcatccc gttcgaggct
     4801 gggccatgcc ggcggggcgc cgcgttccag gcgatgccag tggccgaact cgcgcagcgc
     4861 gtgctggtag tttcggcgcg tgtacggcga ggccgcccga tccacggcca gatgctcgag
     4921 gaactgggcc acgtgcgggt cgggcgattc gtccggttcc ggcggcgggg cgggggcgat
     4981 gggagtgatg gcggtctgtg cgctcacgag gcgcgggcgg tcaggcgtcc agggcgccgt
     5041 cccggggggc gtccgacgat ttggaccagg gcctgttgga ggaccagggc ttcctcgagt
     5101 tgactggaca cgaggcggcg gttggagacg agcagcacgt ccatcgcccg caccaactcc
     5161 tcggccgtgt actgtccggc ctggggcagc gccttgaaga ggacgtacgg atgcatggcg
     5221 gccgggttgt agcgcttgtc gtcgggcatg gcttcggacg gcagccgccc caactgagac
     5281 ttgaagcggt tgtagtcgac gtccggcgcg agcaggccga gtcggaacat ctccttgagg
     5341 aggagcagcg tgcgtacctt cgagatgagc ccatacaaaa gcccgatcgc ggtcttctgg
     5401 cgatcgaact gcatctccca caattcctcg tcgaggcaac ggagcgcgcg gggcagatcg
     5461 cggtctccga gggcttcgcc cagggcgaag gcgcgcgcct gtttgttgcg gctcacgacc
     5521 gcatggacgt cggccacggt gatttcgggg cgtccggcgg cgtagagcga gagcttctcg
     5581 atttcctgcg acaactggcg caggttgggg cccaccgccg acaccagttc gccgagagct
     5641 tcatccgaca tctcctgccg gcgcgtccgc aattcgcctc ggacgaatcg ctccgcccga
     5701 tccgcccagt ccttgtcgtc ggcggacagt ccggcgaggt actcgacggc cccgagtttc
     5761 tcgatggttt tcgagaaggt tttccggcgg tcgacttttc cggcggtgat gagcaggcga
     5821 acgttgtccc acgggaagcg cttgagttcc tgggccaggt cgttcaggga cacggtgacg
     5881 gcctgggcgc cggcgacgcg ttcgtctccc aggaaattgc attggcgcag ccagatgact
     5941 ttgccgctgc cgaagaaggg gagggtttgg agcgcctcgc ggagtctggc cagggaccgc
     6001 agcgcctctt ggctgttcga gaccgtggcg tcaatggtct catggtccat cccgccaatc
     6061 tccttgcacc agtccgcata caattgccga ccccgctgct gcacggcgaa atcgtcctcg
     6121 ccgcacacga gcaccagggg tgcagagggc ttgcgcacgg atgttttcac aggatgcccg
     6181 ccccggcccg catgggtccg cgttttggag cctctggcgc cgcggtggtc acaactcggt
     6241 gaaacatgcg ggttaagggt ttgtgcaaaa cacaattccg gttttggcgg gagcgccacc
     6301 gggccagatg cgaggcccga gggagggagt
//
LOCUS       DAOQRO010000197         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12358_length_6317_cov_5.591025, whole genome shotgun sequence.
ACCESSION   DAOQRO010000197 DAOQRO010000000
VERSION     DAOQRO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12358_length_6317_cov_5.591025"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKK20_08310"
     CDS             <1..271
                     /locus_tag="PKK20_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNU99929.1"
                     /translation="AGKVVLDAEGIRRELKRKRQLKLGQVLRLRVRYFSDGVVLGSRN
                     YVNEVFREFRDRFGARRRDGARPMRGLGALGELATMRDLKVNVVG"
     gene            459..986
                     /locus_tag="PKK20_08315"
     CDS             459..986
                     /locus_tag="PKK20_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99930.1"
                     /translation="MNPTSAFKLVETVRQEARFALLAFQNLRVALNEADQERIFLFAH
                     AMAHHAGAVGCFLAPSRTSSTPRGEFLRIQLKVEGGSPLLMPALRDMLKRAADEEYED
                     WVGSLEKLDYLEMSVMPQGALAGSSPDVFQRRIDPDSLHFGYRNHSLDLSRLAQELRR
                     FEGACQYWLREHSPW"
     gene            1249..1527
                     /locus_tag="PKK20_08320"
     CDS             1249..1527
                     /locus_tag="PKK20_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNU99931.1"
                     /translation="MRLPWPSRRVSQGVNVLVVAGGGAGRLDHMVQVLTQGAADAVLA
                     ASVFHFGRCTVADVKQHLAAHGIPVHAPASSLLPRTGAVSTGSNGSHT"
     gene            1524..1913
                     /gene="hisI"
                     /locus_tag="PKK20_08325"
     CDS             1524..1913
                     /gene="hisI"
                     /locus_tag="PKK20_08325"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNU99932.1"
                     /translation="MSFFDELKFNAEGLIPAIVQEESSGRVLMLAWMNRVALEKTLEL
                     GQTVFWSRSRRKLWIKGETSGHVQTVKSVAFDCDGDTLLIEVEQAGAACHEGYKSCFY
                     RAVEDGGSRIRITEPRLRTPDDIYGKQ"
     gene            1927..3492
                     /gene="trpE"
                     /locus_tag="PKK20_08330"
     CDS             1927..3492
                     /gene="trpE"
                     /locus_tag="PKK20_08330"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNU99933.1"
                     /translation="MHCPTRSEFARLAKHGNLIPVARRVLADYETPLSAYHKIRGSEE
                     SFLFESVEGGEHLGRYSFVGCAPRAVILQHGDTIELREHGRRTAEWKVSGSGKDEPGR
                     VRDGLVAVADVLGRFHPVSVPGLPRFTGGAVGYLGYEFIHDVEPVVPRPVGNALGTPV
                     LYFLIADQLLVFDRVTQTLTVMVNAVIEPGADAGRAYDEAVTEIERLVSLLEQPSHHP
                     AVTADSDAPPLAFSSNQPRERFIANVLKAKEYITAGDIIQVVGSQRFSARVRATPLEI
                     YRAARTINPSPYMFLLELDGFALVGASPEIHVRCEDRRVEIRPIAGTRPRGRTAEEDA
                     AQERELLADPKERAEHVMLVDLARNDLGRVCEYGSVKVRDLMVIEKYSHVMHIVSQVE
                     GRLAVDKTPFDLMRATFPAGTLSGAPKIRAMQIISELEQTQRGPYGGCVGYFSFGGNL
                     DCCITIRTALLKDGQAFVQAGGGWVSDSKPENELAETLNKSKAMFKALAMAESFTGQG
                     SQAAPRADASSRG"
     gene            complement(3481..4554)
                     /gene="lpxD"
                     /locus_tag="PKK20_08335"
     CDS             complement(3481..4554)
                     /gene="lpxD"
                     /locus_tag="PKK20_08335"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNU99934.1"
                     /translation="MPYTVAEIARHLEGQVVGDGSIPLDGFAPADTARPGDLTFAENE
                     AYFARAEQSAASAILVAGEWASATKALIRVPDARIAFAKVLPLFHPEPIFAPGVHATA
                     LVAPTAEVHASAHVGPYCVVGEGARIGARTVFEAMVHVGAQVRIGEDARFYPRVTVYP
                     ESRIGNRVVVHAGAVIGSDGYGYVTDGGVHRKIPQVGNVVINDDVEIGANVTIDRGAL
                     GSTVIGRGTKIDNLVQVAHNVVVGEHCLLVAQVGIAGSTRLGSYVTLAGQAGLAGHLK
                     IGDQVIVAAQSGVMRDIPDKGQWMGSPARPDWQMKRQLIALDRLPDLLKRIADLERRI
                     ETIGRTLDTEAGPERKTPPASTP"
     gene            complement(4568..>6317)
                     /gene="shc"
                     /locus_tag="PKK20_08340"
     CDS             complement(4568..>6317)
                     /gene="shc"
                     /locus_tag="PKK20_08340"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNU99935.1"
                     /translation="AINHLFSLQLPDGGWNIYHGGPAEVNATIKAYLALKLAGVPETH
                     LRMLRARSVALNLGGVPRMNTFSKLYLALLGLFPWKYVPTIPCEVLLIGKWFHVNFWE
                     MSNWSRAMLVPLAIINHFKPTRPLGQGISLDELYPEGYHDRDLRLQRDPQILTTRNFF
                     IWLDRLHKFAELFAHAGIHPFRRRALKRAEEWMLERFEGSDGLAAIFPAMLNSLIALK
                     ALGYPDDHPQVVRTEQHLKGLEHETSDTVRIEPCFSPVWDTAITLIALAESGVPPEDP
                     RLQRATEWLLSKEIRFRGDWRHKNPANVEPSGWVFEFENKWNPDVDDTAMVLLALRLV
                     PTSDSRRDEAYERGLKWMLTFQCKDGGWAAFDKDCTKNVLTKVPFADHNAMLDPECAD
                     ITARILEVLGHERFPLNHPQVVKAIEYLRDQQEPDGSWYGRWGVNYLYGTWQVLRGLH
                     TLGIDMHQPWLLQARDWLESVQHKDGGWGERVDTYEDPVFKGRGPSTASQTAWVVMGL
                     CTFGDPNRPSLIRGVEYLVRTQNRDGSWSEDEITGTGFPKVFYLKYDMYRNSWPLLAL
                     ATYRRLSDGLAPERRH"
BASE COUNT         1124 a         1805 c         2188 g         1200 t
ORIGIN      
        1 tgcgggcaag gtggtgctgg atgcggaggg aatccggcgt gagttgaaga ggaagaggca
       61 gttgaagctg ggacaggtgt tgcggctgcg ggtgaggtat ttcagcgacg gagtggtgct
      121 ggggtcgcgg aactacgtga acgaggtatt ccgggagttc cgggatcggt ttggggcgag
      181 gcggcgtgac ggggcgcgac cgatgcgggg cttgggagcg ttgggggagt tggcgacgat
      241 gcgggatttg aaggtgaatg tcgtggggta attcagcaga cggatgggaa agacattgac
      301 tgctcgaatc gaggagcgat gcggtttcga gttgtcgatg tcgtagcggg gatgtcgtac
      361 agcttggcaa tccaatcgtc gtgatggtag ggtgcggtct ggagagcggc tccttcgagc
      421 attcgccgct gttccgcacg acagaagatg agcagactat gaatccgacc tcagcgttta
      481 agctggttga aaccgttcgc caggaggctc ggttcgcctt gctggccttt cagaacttgc
      541 gcgttgccct caacgaagcg gatcaggagc gcatctttct ctttgcgcat gccatggcac
      601 accacgcagg cgcggtgggg tgttttttgg ctccgtcgcg cacatcatca acgccacgcg
      661 gcgagtttct tcgaatccaa ctgaaggtcg agggcggcag cccgctgctc atgccggcgc
      721 tgcgcgacat gctcaagcgc gcagcagacg aggagtacga agactgggtg ggtagcttgg
      781 agaagctcga ctacctcgaa atgagtgtga tgccacaggg cgcgctggcg ggttcgagtc
      841 cggacgtctt ccagcgccgg attgatccgg attcactgca ctttggttat cggaatcatt
      901 cgctggattt gagccgcctg gcgcaggaac tgcggcgttt cgagggagcg tgtcaatact
      961 ggctgcgcga gcactcgccg tggtaagggt cttccaaaac cgggaaaagg ctgtggatgg
     1021 ttcccggcat cagggttatt cgagcgcggc attgcaggga cggcggggaa gtgtttggtc
     1081 tggcgggggc atgtggcaat ctccttgtca acccgggcat cggaggctat atgcagcgtc
     1141 cgatgctcgc caaacgggtc attccttgcc tggatgtgca cgaccgacaa gtcacgcgcg
     1201 gtgtccggtt cggccgggcg gaggcgggcg aattgggcaa tgtcggggat gcggttgccc
     1261 tggccctcac gccgtgtcag tcagggtgtg aacgtgctgg tggtggccgg cggcggcgcg
     1321 ggccgcttgg atcacatggt ccaggtgctg actcaaggcg cggcggatgc cgtgctggcg
     1381 gccagcgtct ttcattttgg cagatgcaca gtcgcggatg tcaaacagca cctggccgct
     1441 cacggcattc cggtgcatgc gcccgcgtcc agcctcctgc cacggaccgg cgcggtctca
     1501 acaggtagca acggatcaca cacatgagtt ttttcgacga attgaagttc aacgccgagg
     1561 gcctgattcc ggccattgtg caggaggaat cgagcgggcg ggtgctgatg ctggcctgga
     1621 tgaaccgagt ggcattggag aagacgcttg aattgggcca gacggtgttt tggagccggt
     1681 cccggcggaa gttgtggatc aaaggcgaaa cgagcgggca tgtgcagacg gtgaagtccg
     1741 tcgcatttga ttgcgacggc gacactttgt tgatcgaagt cgagcaggcg ggcgccgcgt
     1801 gccacgaggg gtacaagtcc tgtttctatc gcgccgtcga ggacggtgga tcgcggattc
     1861 ggatcacgga accgcggctg cggacgccgg acgatatcta cggtaagcag tgagagtcct
     1921 gccgcgatgc actgccccac acggagcgaa ttcgccaggc ttgccaaaca cggcaacctg
     1981 attcccgtgg cccggcgtgt gcttgccgac tatgagacgc cgctctcggc ctaccacaag
     2041 atacgcgggt cggaagagtc ctttctgttc gagtcggtcg aggggggaga gcatcttggg
     2101 cgctattcgt ttgtcgggtg tgcgccgcgg gccgtcatcc tccagcatgg ggacacgatc
     2161 gaactgcgcg agcatggacg ccggacggcc gagtggaagg tgagcggaag tgggaaggac
     2221 gaaccgggcc gggtgcggga cgggttggtg gcggtcgccg atgtcctcgg gcggtttcat
     2281 ccggtgagcg tccccggctt gccgcggttt acgggcggcg cggtagggta tctagggtac
     2341 gagttcatcc acgatgtaga acccgtggtg ccgcgtcctg tggggaacgc cctgggcact
     2401 ccggtcctgt atttcctgat tgcggatcaa ctgcttgtgt tcgatcgcgt gacgcaaacg
     2461 ttgaccgtga tggtgaatgc ggtgatcgag cccggcgccg atgcgggtcg ggcgtatgac
     2521 gaggccgtga ccgagatcga gaggttggtg tcactgcttg aacagcccag ccatcacccc
     2581 gcggtcacgg cggattccga tgcgccgccg ctggcgttca gctcgaacca gcctcgcgag
     2641 cgctttattg cgaatgtgct caaggcgaag gagtacatca ccgcaggcga catcatccag
     2701 gtcgtcggct cgcagcgatt ctccgcccgt gtgcgggcga ctcccctgga gatctaccgc
     2761 gcggcgcgca ccatcaatcc ttcgccctac atgtttttgc tcgagttgga cggtttcgcg
     2821 ctggttgggg cgtctcccga gatccacgtt cgctgcgagg atcggcgcgt cgagattcgc
     2881 ccaatcgcgg ggacccgtcc acgcgggcgg accgctgagg aggacgcggc gcaggagcgg
     2941 gaattgctgg ccgaccccaa ggaacgcgcc gaacatgtca tgctggtgga tctggcgcga
     3001 aacgacctcg ggcgcgtctg cgagtatggc agcgtaaagg ttcgggacct gatggtgatc
     3061 gagaagtaca gtcacgtcat gcacatcgtg tcgcaagtgg aaggccggtt ggcggtcgac
     3121 aagaccccgt tcgacctgat gcgagccacg tttcccgcgg gaacgctctc gggggcgccg
     3181 aaaatccggg cgatgcagat catttccgaa ctcgagcaaa cgcagcgggg gccttacggc
     3241 gggtgcgtcg ggtacttctc atttggcggg aacctcgatt gctgcatcac gatccggacc
     3301 gcccttctga aggacggtca agcgttcgtg caggcgggcg ggggctgggt gagcgattcg
     3361 aagccggaga acgagctggc ggagacgctc aacaagtcga aggcgatgtt caaagccctc
     3421 gccatggcgg agagcttcac ggggcaggga tcacaagccg cgccccgcgc ggatgcgagt
     3481 tcaaggggtt gacgcgggcg gtgtcttgcg ttcgggcccg gcctcggtat cgagcgttcg
     3541 cccgattgtc tcgatgcgac gttccaggtc cgcgatgcgt ttgaggaggt cggggagccg
     3601 gtccaaggca atgagctggc gcttcatctg ccagtccggg cgcgcgggcg atcccatcca
     3661 ttggcccttg tcggggatat cgcgcatcac gcccgattgc gcggccacga tgacctggtc
     3721 gccgatcttg agatggccgg cgaggccggc ctgcccggca agcgtgacgt agctgccgag
     3781 acgggtgctg ccggcgatgc cgacttgggc gaccagcagg caatgctcgc ccacgaccac
     3841 gttgtgagcg acctggacca gattatcgat cttggtgcct ctgccgatca cggtggatcc
     3901 gagcgcccca cgatcgatcg tcacgttggc cccgatctcc acgtcgtcgt tgatcaccac
     3961 gttgccgact tggggaatct tgcgatgaac tcccccgtcc gtcacgtagc cgtagccgtc
     4021 cgaaccgatg acagcgcccg catggacgac gacgcggttg ccgatccggc tttcgggata
     4081 gaccgtgact cgcgggtaga accttgcgtc ttcgccgatg cgcacttgcg ctccgacgtg
     4141 caccatggcc tcgaaaaccg tgcgcgcgcc gatccgcgcc ccttcaccga cgacgcagta
     4201 agggccaacg tgcgcggacg catggacctc ggccgttggc gccaccaggg cggtggcgtg
     4261 aacgccgggc gcgaatatcg gttcggggtg gaagagcggc aggaccttgg cgaacgcgat
     4321 gcgcgcatcg gggacgcgga tcagggcttt ggttgcggat gcccactcgc ccgccacgag
     4381 gatggcgctg gcggcgctct gctcggcgcg agcgaagtac gcctcgtttt ccgcgaaggt
     4441 cagatcgccc ggacgggcgg tgtcggcggg cgcgaagccg tccaagggaa tcgagccatc
     4501 gccgaccacc tgaccttcga ggtggcgcgc gatttcggcg acggtgtaag gcatagcgga
     4561 ggggggctca gtgcctgcgc tccggggcga gcccgtcgct caagcggcga taggttgcca
     4621 gggcgagcag cggccaactg ttacggtaca tgtcgtattt caaatagaag accttgggga
     4681 agccggtgcc cgtgatttcg tcctcggacc aggatccatc ccggttctga gtgcggacca
     4741 ggtactcgac gccgcgaatc aggctgggac ggttaggatc gccgaaggtg cacagcccca
     4801 tcacgaccca cgcggtctgc gaggcggtgc tgggtccgcg ccctttgaac acaggatctt
     4861 cgtacgtatc gacgcgctcg ccccagccgc cgtctttgtg ctggacgctc tcgagccaat
     4921 cgcgcgcttg gagcagccac ggctggtgca tgtcgatgcc gagcgtatgc aatccgcgca
     4981 ggacctgcca tgtcccgtag aggtagttca cgccccagcg tccgtaccac gaaccgtcgg
     5041 gctcctgctg atcgcgcaga tactcgatgg ctttgacgac ctgcgggtgg ttgagcggaa
     5101 accgttcgtg tcccaggacc tccaagatgc gcgccgtgat gtcggcacac tcggggtcaa
     5161 gcatcgcatt gtgatcggcg aagggaacct tggtgaggac gttcttggtg caatccttgt
     5221 cgaacgccgc ccaccccccg tctttgcact ggaaggtcag catccatttg agcccccgct
     5281 cgtaggcttc atcgcgccgg gaatcgctcg tcgggaccag gcgcagcgcc aggagcacca
     5341 tcgccgtgtc atccacgtcg ggattccact tgttctcgaa ctcgaacacc caaccgctgg
     5401 gctcgacatt cgccggattc ttgtgccgcc aatctccgcg aaaccggatt tccttgctca
     5461 gcagccactc cgttgccctc tgcaggcgcg ggtcctcggg cggcacaccc gattccgcaa
     5521 gggcaatcag cgtgatcgcc gtgtcccaga caggcgaaaa acagggctcg atgcggaccg
     5581 tatcggacgt ctcgtgttcg aggcctttga ggtgctgctc ggttcggacg acttgcgggt
     5641 ggtcgtccgg ataaccgagg gccttgaggg cgatcaggga gttaagcatg gcggggaaga
     5701 tggcggccag gccatccgag ccctcgaaac gctcgagcat ccattcttcg gcgcgcttca
     5761 acgcccgccg tcggaaaggg tggatgccag cgtgcgcgaa gagttcggcg aatttgtgca
     5821 ggcgatcaag ccagatgaag aagttccgtg tcgtgaggat ttgcggatcc cgctgcagtc
     5881 gcagatcgcg gtcgtgatag ccttccgggt aaagttcgtc gagggagatg ccctgtccca
     5941 agggccgagt cggcttgaaa tggttgatga tggcgagggg aacgagcatg gcccgcgacc
     6001 agttgctcat ttcccagaag ttgacgtgga accactttcc gatcagaagc acttcgcagg
     6061 ggatcgtggg cacatacttc cacgggaaca acccgagcag cgcgaggtaa agcttggaaa
     6121 acgtgttcat ccggggcacg cctccgagat tgagcgccac cgaacgcgcg cgcagcatcc
     6181 ggagatgcgt ctccgggact ccagccagct tcaacgccag ataggccttg atggtggcgt
     6241 tcacttcggc gggaccgccg tgatagatat tccatccgcc gtcgggcagt tgcagcgaga
     6301 agagatggtt gatcgcc
//
LOCUS       DAOQRO010000198         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12360_length_6317_cov_4.324976, whole genome shotgun sequence.
ACCESSION   DAOQRO010000198 DAOQRO010000000
VERSION     DAOQRO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12360_length_6317_cov_4.324976"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKK20_08345"
     CDS             complement(<1..1036)
                     /locus_tag="PKK20_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HNU99936.1"
                     /translation="MRQDAHWIQRLLAPLCLLALPFALTQAGGSGLDVAVVVNSHSAN
                     SMALGNYYRERRQLPPRNIVRIAWTGSRTDWTRTQCQNLLVNPLLTALTERRLASQIR
                     FVVLSMDIPYRVIDGRDVNSTTSVLFYGFKTNDWRTSSPCRISPEGNNSYFATERAFG
                     SQPDTTNEPRFLATMLTAEILAEAMQTVDRGVASDGAFPTQTVLLQKTTDSARNVRFP
                     NFDNAILDMQINGLFPLIATNSNQTSGLSDLLGMQTGLAQFSVTKDTFVPGAMADSLT
                     SYGGCLFEPCGQTNLLAFLRAGATASYGTVVEPCNYTEKFPDPSVYFYQARGFTIAEC
                     CYQGLASPYQG"
     gene            1626..2441
                     /locus_tag="PKK20_08350"
     CDS             1626..2441
                     /locus_tag="PKK20_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNU99937.1"
                     /translation="MKSTVPVASRSRSVGGFTLIELLVVIAIIAILAGMLLPALARAK
                     DKGQRISCMNNLKQVSLFLQLYTDDYNDIFPAHRNQNVNNADEIVSLTNWWGTTIIGY
                     ARNQSNLFRCPAIKGKRLDNGLRWEWKFDCHKVGYGINSWFLSLWPYTDQSLTVGGIR
                     FETKPWFKRTSIVTPTDTLMIGDSMPKIDGMWSSSCYWPWACMDPKNSQYGGYEGIDR
                     NRHRDTGVVVFTDGHAEARKDAKINPPRDPGFGDARGLINSRYWDPLKRGGDR"
     gene            2729..3418
                     /locus_tag="PKK20_08355"
     CDS             2729..3418
                     /locus_tag="PKK20_08355"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNU99938.1"
                     /translation="MKHADQPENLMPVRIKICGITSIEDAVSALNAGADALGLMFYPP
                     SPRCVSLDAASAIVACVPPTVATVGVFVDPEADFVREAIARTGLNTLQFHGNETPEFC
                     AQFGLKTIKAFRLRNRAALDGVRAHSLSAWLLDSCVPGVPGGSGQTFNWDLAVEAKRW
                     GNPVFLAGGLTPENVRDAVRRVAPFGVDVSSGVEASPGRKDPVRLRRFIAEARAGADE
                     SPMALGLPQRV"
     gene            3449..4489
                     /locus_tag="PKK20_08360"
     CDS             3449..4489
                     /locus_tag="PKK20_08360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99939.1"
                     /translation="MNEALRQESRRLEQSWTRHSAQMLETYLVADVEDPRINVQSLLT
                     RHFLIFALSGRHFEMADAELRFAACLNWILRFAKENPDVDAMAGVRHALQCGADNAEG
                     VELPHFLRATYRALSAGAEGGAAPDYVSAALEGVAFDGRRPRLPASVLDTFMDLWRTV
                     LSSETSPARRVLEPACGSANDYRFLEACGLASRVDYTGFDLCEHNVRNARKLFPRAHF
                     EVGNVFEIAAGDRSYDHSFVHDLFEHLSPEGIRAAAKELCRVTRRSLCLHFFNMDEID
                     AHVVRPVEEYHWNTLSLDRMTELFAEEGFNGRGYHIGTYLRRRVGCEHTHNPRAYTLI
                     LGASTPAADRAR"
     gene            4777..5412
                     /locus_tag="PKK20_08365"
     CDS             4777..5412
                     /locus_tag="PKK20_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNU99940.1"
                     /translation="MEDAAEVAHKSTGQKVAEYLRGHGASKTTTIIAISTLPIVELRG
                     AIPVGHILYPDMEKSTRLGRDDLERSARIFVYAVVGNMLPVPFILLLLGPISRICMKV
                     PWGKRFFDWLFARTRRRTADIEKYETLGLTVFVAIPLPATGAWTGALAGWLLGMSFGH
                     CLLSIFLGVAIAGVIMTVLALMGWIGAAIAGVVLLAFFLGILAKALKAADL"
     gene            5985..6110
                     /locus_tag="PKK20_08370"
     CDS             5985..6110
                     /locus_tag="PKK20_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99941.1"
                     /translation="MLDHVKRWGGRATPDSVGYALVRQFRNQAIHLVLEPVTTRS"
BASE COUNT         1115 a         1902 c         2056 g         1244 t
ORIGIN      
        1 caccctgata cgggctggcc aggccttgat agcagcattc ggcaatggtg aagccgcggg
       61 cctggtagaa atagacgctc gggtccggga acttctccgt gtagttgcac ggctcgacga
      121 ccgtgccgta gctggccgtg gcgcccgcgc gaaggaacgc caggaggttc gtctggccgc
      181 atggctcgaa gaggcagcct ccgtaggagg tgaggctgtc cgccatcgcc cccggcacga
      241 acgtgtcctt ggtgacgctg aactgggcga gaccggtttg catgcccaac aggtcggaca
      301 atccggaggt ttggttcgag ttggttgcga tcagcggaaa caagccgttg atctgcatgt
      361 ccagaatcgc attgtcgaaa ttcgggaacc gcacattccg ggctgagtcg gtggttttct
      421 ggagcaggac ggtttgggtg gggaaggcgc cgtcgctggc cacgccgcga tccacggtct
      481 gcatggcctc tgcgaggatt tccgcggtca gcatggtcgc cagaaagcgc ggttcgtttg
      541 tcgtatcggg ttgggagccg aacgcccgtt cggtggcgaa gtacgagttg tttccttcgg
      601 gcgagattcg gcaggggctc gaagtccgcc agtcgttggt cttgaatccg tagaacagca
      661 ccgatgtcgt gctgttcacg tcacgtccgt cgatcaccct gtatggaatg tccatcgaga
      721 ggacgacgaa tcggatctgg ctggccaggc ggcgctcggt caacgccgtg agaagcgggt
      781 tcaccagcag attctggcac tgcgtgcgag tccagtcggt cctgctgcct gtccacgcga
      841 tgcggacgat gttccgcggg ggcaactggc ggcgttcgcg atagtagttg cccagcgcca
      901 tggaattggc gctgtgcgag ttcaccacca ctgcgacatc caggccgctg ccgccggcct
      961 gagtcagcgc aaacggaagg gccagcaaac acagcggcgc gagcaggcgt tgaatccagt
     1021 gggcgtcttg tctcatgatc cgttgatcct gttggccagg cgcggtcgtt gtcggcccgc
     1081 ggactgtgcc cggtggaact gctgcccgca agcctcaagg agacgcctcg tgttgtcgga
     1141 gggacgatgg cggaatgccg gatggtgcat ctggtttttg tcggtgttct gtcgcccggt
     1201 gagggtgccg ggcctacaac ggatcgagat tgccggaggg gcggcgcctg tctgccgcct
     1261 gcctgccggc agggcagaca ggtcctcgtc gcccgccaac agggtttgca ggtctcttcg
     1321 ccggggacga ggacgccccc actccggcct ccgactcacg gacagcttaa gccggcagca
     1381 agccggcgcc ccggccttcg tgtgtgtgag cattgcactc caggccgcgt accctgtctg
     1441 cgccgcaaag cgcagcggcc tgtctgccgt gtcgggcacg gcacaggcag gcgcggcagg
     1501 caggcacccc cgcggcgcgt gctcgattcc caccgatgac cttcagatgc actgaacggg
     1561 gcgccaaggg cgctcttttt cattcgcgtt tcgggctcga gtcggctaga gatagggagt
     1621 ttccaatgaa atcgaccgtg cctgttgctt ctcgaagccg ttcagtcggg ggtttcacgc
     1681 tgatcgaact gctggttgtc atcgccatca tcgccattct ggccggcatg ctgttgccgg
     1741 cgttggcgcg cgccaaggac aaggggcagc gtatcagttg catgaacaac ctcaagcaag
     1801 tcagcctttt cctgcaattg tacaccgacg attacaacga catctttccg gcccaccgaa
     1861 accagaacgt gaacaatgcc gatgaaatcg tctcgctcac caactggtgg ggaacgacga
     1921 ttatcggata cgcccgaaac cagtcgaatc tgttccggtg tccggccatc aagggcaagc
     1981 gcctggacaa cggcctgcgc tgggagtgga agttcgactg ccacaaggtc gggtacggca
     2041 tcaattcgtg gttcctcagt ctgtggccgt acaccgatca aagcctcacg gtcggcggca
     2101 tccgattcga gacgaaacct tggttcaagc gcacgtcgat cgtgacgccc accgacacgc
     2161 tcatgatcgg cgactccatg cccaagatcg acggcatgtg gagcagcagt tgctactggc
     2221 cctgggcctg catggacccc aaaaactccc agtacggcgg atacgaggga atcgaccgca
     2281 accgccatcg cgacaccggc gtggttgtgt tcacggacgg ccatgccgaa gcgcgcaaag
     2341 acgccaaaat caaccctccg cgcgatccgg gcttcggcga cgctcgaggt ctgatcaatt
     2401 ccaggtattg ggacccgctc aagcgcggcg gcgaccgcta gcccgcatat ttcatactcc
     2461 atcctgcaca gggcgatcag acttcgcaag aggactgcta tgcaggctag aaaagcaaaa
     2521 ccgcgccagg cttgagcgac agagatatgc aacatactgt tcctatcgaa gaacgccttc
     2581 acgaccgaca gaatgctcgt ggtccctttc tgggcgcgat tttgccaact agctcggtca
     2641 cggttcctcg cgaagcgtgt gaaataatcg ggctggcccg catgggtcca cagcgtcgag
     2701 ttgtcggcgt tccccaggtc acaaccccat gaaacatgcg gaccagcccg agaatctcat
     2761 gccggtccgg atcaagattt gcgggatcac atcgatcgaa gatgctgtga gcgcactgaa
     2821 tgccggcgcg gatgcgctgg gcctgatgtt ttatccgccc agcccccgtt gcgtttccct
     2881 cgacgcagca agtgccattg tcgcatgcgt gccgccgacc gtggcgacgg ttggtgtttt
     2941 tgtggacccc gaggcggact tcgtgcgcga agccatcgcc cgcactggac tgaacacgct
     3001 gcagtttcat ggcaacgaaa cacccgagtt ctgcgctcaa tttgggttga aaacgatcaa
     3061 ggccttccgt ttgcgcaacc gcgccgcgct cgacggagtc cgcgcccact cgctttcggc
     3121 ctggttgctg gacagttgtg tcccgggcgt tccgggcggc agcgggcaga cgttcaactg
     3181 ggatctggcc gtcgaggcaa agcggtgggg caatccggtc tttctggcgg gaggactgac
     3241 tccggagaat gttcgggacg cggtccggcg cgttgcgccg tttggggtgg acgtatcgag
     3301 cggtgtcgag gcttcgcccg gacgcaagga tcccgttcga ctcaggcggt tcatcgccga
     3361 ggcacgggcc ggagcggacg aatcgccgat ggccctcggt ttgccgcagc gggtctgagc
     3421 cgcaagaccg aacaccgcgg gattggccgt gaacgaagcc cttcgccagg aatccaggag
     3481 actcgaacag tcctggacgc gtcacagcgc gcagatgctc gagacctacc tggtcgccga
     3541 tgtcgaagac ccgcgcatca atgtccagag cctcctgacg cgtcatttcc tcatcttcgc
     3601 cttgtcgggg cggcatttcg agatggccga cgcggagctg cgctttgcgg cctgcctcaa
     3661 ctggatactt aggttcgcca aggagaaccc cgatgtcgat gccatggcgg gcgttcggca
     3721 cgcgctgcaa tgcggcgccg ataacgctga aggggtggag ttgccgcatt ttctccgcgc
     3781 gacttatcgt gcgctttcgg cgggcgcgga gggcggggcc gcgccggact acgtgagcgc
     3841 ggctctcgag ggcgtggcgt tcgacgggag gcggccccgc ctgcccgcca gcgttctgga
     3901 tacgttcatg gacctctggc gcacagtgct ttcctccgaa acatcaccag cgcggcgtgt
     3961 gctggagccg gcctgcggca gcgccaacga ttaccgcttc ctggaggcct gcgggcttgc
     4021 gagccgggtg gattacaccg gattcgatct gtgcgagcac aatgtgcgga atgctcggaa
     4081 gctctttccg cgcgcgcatt tcgaggtcgg gaacgtcttc gagattgccg ccggcgatcg
     4141 ttcatacgat cattcgttcg tgcatgatct cttcgagcat ctttcccccg aggggattcg
     4201 ggcggcggcg aaggaactgt gccgtgtgac gcgccgcagc ctctgtctgc acttcttcaa
     4261 catggatgaa atcgatgccc acgtcgtgcg ccccgtcgag gagtatcatt ggaacacgct
     4321 gagtctggat cgtatgaccg agctgttcgc ggaggaggga ttcaacggtc ggggctacca
     4381 tatcggaacc tacctgcgcc ggcgtgtcgg ctgcgagcac acgcataatc cccgcgctta
     4441 cacgctgatt ctgggggctt cgactccggc cgcggatcga gcacgatgaa agccatccga
     4501 aggagaatat tttccatact gaagggtccg cggcatacgc gacaaagact cctcaccccg
     4561 tccctctccc atccgacctg cctgccgaag cccgggcgca ggcaggtggg agagggtgcc
     4621 cggagggcgg gtgagggtga cggtgtctca catcccacgg attcctctgc aaacgtcctg
     4681 cgtgtcctgc tgtttctcgc actttcatgc ccggttgttt ggtctgcggt tccggaaatc
     4741 gcagggcctg aaactcgaga aggcgagcgc gcggcggtgg aagatgccgc ggaggttgcg
     4801 cacaagagca cgggccagaa ggtcgccgaa tacttgcgcg gacacggggc ctcgaagacc
     4861 accacgatca tcgccatctc caccctgccg atcgtcgaac tgcgtggcgc gatcccagtc
     4921 ggacacattc tctatccgga catggagaaa tcgacccgtc tgggccggga cgacctcgag
     4981 cgatctgcgc ggatctttgt ctatgcggtg gtgggcaata tgttgcccgt gccgttcatt
     5041 ctgctgctgc tcgggccgat atccaggatc tgcatgaagg tgccgtgggg caagaggttc
     5101 ttcgattggc tctttgcccg cacccggcgc aggactgccg atatcgaaaa gtacgagacc
     5161 ttgggtctga ccgtgttcgt cgcgattcct ctgccggcca cgggcgcctg gaccggggct
     5221 ttggctggct ggctgttggg aatgagcttc ggtcactgcc tgctttccat ctttctcggc
     5281 gtggcgattg ccggtgtgat catgaccgtc cttgcgctga tgggctggat cggcgccgcc
     5341 atcgccggcg tcgttctgct ggccttcttt cttggcattc tggcgaaggc gctgaaggct
     5401 gcagacctgt gacgggcggc ttgattcaat ggatggcaga cgtgacggtc ggtgattatg
     5461 aaggagcttg aacagcggtt catgaagcgg atcaaccggc agcggaagcg gtctggcacg
     5521 ttggggcgaa gcggttcgcg aatgctgccg acatcggttg ggcgaggtcg cgcgcctggc
     5581 cctggcgcga gcgcctgccg atcacagggc tttggcggaa gcctaacttg acggagtgaa
     5641 cgcggggctg gcggccttgg gcgccgcgcc gcccgagtat ctgctcctgc gctcgactcc
     5701 gcgttcctgg ctgcccgagg gctcgattct ggtgggttac tcgatgtttt tcaccctgca
     5761 agagggcgac ggagctggag agaggtcgtt gggctgcgtt cttcgagaat tgcttccagc
     5821 cgaggcattc gcgttctttg cgccgattgc gaccgactgg gatgcggcgg tggacgacac
     5881 cgtactcgag aatccgccgg tgccgtcgac aaacgtgttc tccttcgagt catcggggca
     5941 ggggagcaac ttggacgact cccaaccggt ccgaggttgc cggcatgctc gatcatgtca
     6001 agcgctgggg cggacgcgcc acgccggact cggtcggata cgcgctggtc cgtcaattcc
     6061 ggaatcaggc gatccatctg gtgctcgagc ccgtgacgac ccggtcctga ccatgcaaac
     6121 cgcacgcatc cgatcatcgt gactccagca atgaacgagg accaacccct ctcgaccatc
     6181 gaaacctccg atggcgtcgc ccgcttcgtc acgacccgct ggagcgtggt gcgtcggctc
     6241 tgggacccgc tctcaggaca gcctgtgcgc ggcggtggga acatggccca atacgggcgg
     6301 tttgaaaatg gaccggg
//
LOCUS       DAOQRO010000199         6276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12480_length_6276_cov_4.871886, whole genome shotgun sequence.
ACCESSION   DAOQRO010000199 DAOQRO010000000
VERSION     DAOQRO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12480_length_6276_cov_4.871886"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(429..1430)
                     /locus_tag="PKK20_08375"
     CDS             complement(429..1430)
                     /locus_tag="PKK20_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99942.1"
                     /translation="MISESVFRSAGAEGSGFLVAVLLGGLICVCRGAEVEASVMPGLE
                     LVRDPHFSLGFHLLNPTPGRRVVCGRLAPKGSKADPVWDLAQWSSRFPLAAGPAVESE
                     HGVLRWSNAAKGVTVGGGGRNRADLSLAVNASREYVAGARKLGEPWVHLLVQQNFTNS
                     PSLGEIGTARLRIEARLVNSRLHRTHDYTPELHAAQYQMFLILQNSNRRSPGFGRFLW
                     FGISLYDDRARIPPEHKQCDTAGSGMFIYTPPGESFTRRSAHDGEWIQVDRDLRPLLV
                     EALETAWGRGFLDESRDLADYCLTGMNLGWEVPGVFDVEMCVRGLSLVVTTVRASGP"
     gene            1528..2385
                     /locus_tag="PKK20_08380"
     CDS             1528..2385
                     /locus_tag="PKK20_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNU99943.1"
                     /translation="MTLHHLSQRDFLKLTGTATLAATSGLPVGLLAADAKKIPVGLQL
                     YSVREQCQKDIVGTVKAVAKMGYKGVEFAGYYGRDAKTLRQLLDDTGLKCCGTHIGLE
                     TLLGDELSKTIEFNRVLGNPYLIVPGLGEKYTKTRTAWQETAEIFNEIAEKVKPHGMR
                     VGYHNHTTEFHELDGEMPWDTFFGRTRKDVIMQFDTGNAMHGGGDATIYLKKYPGRAA
                     TVHIKPFSKAKPNALLGEDELPWPEIFRLCETVGATEWYIIEYESDAFPPLVSVEKCL
                     EVMRKWGKC"
     gene            complement(2752..3504)
                     /locus_tag="PKK20_08385"
     CDS             complement(2752..3504)
                     /locus_tag="PKK20_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNU99944.1"
                     /translation="MQTAVLVEACANSLTSALAAQAGGAARVELCAALIEGGVTPSAA
                     VIELARQRLDIGLNVLVRPRGGDFCYDDTEVAVMLRDIEFCRRAGVDGVVIGMLTPEG
                     SVDVERTGALVAAARPMQVTFHRAFDMAADADRALDDVLALGVERLLTSGRRGSAFEG
                     RGLIAELVRRAGARLIVMPGAGIHEGNVRELIEATGVREVHLSGQSKIASRMIYRNPH
                     VFMGVPGLPEYEIGVTDADKIRRVVEAANQAA"
     gene            complement(3575..4432)
                     /locus_tag="PKK20_08390"
     CDS             complement(3575..4432)
                     /locus_tag="PKK20_08390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99945.1"
                     /translation="MTYADGHPFHRSGQAALSDLNTAEWIAVFDRLEREAERFRRHES
                     SFRSAEYRWPRDPLHDWSRCWEYPYAYYHLERFIQHAPAASRPVAVDVGSGVTFFPFA
                     LGALGYDVVCTDTDPICRTDLERAVPLVSAAPGRVSFRMIEQGRLPLSEGEADVATCI
                     SVLEHIPDFESTVRELARVVRPGGLAVLTNDLDLRGDHEIGPERYRALRAELLRHFEW
                     VWPEAAVHPADLLRTSNGPYRSETPRGMRRAWWLFREQVVKRMLGRKRWPLLVYDLAV
                     QAFVLRRKA"
     gene            complement(4429..5361)
                     /locus_tag="PKK20_08395"
     CDS             complement(4429..5361)
                     /locus_tag="PKK20_08395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU99946.1"
                     /translation="MNPTDAIPLLPPLPPGVKAGRVPLWPLDGLIHRGCPVCGGDAPR
                     PICRRPDRLVVACCAACGMHYLPDIPSESEMQAFYRNYGEYKGLRPPPEPRWRRWIPW
                     QRPNPFLEILLQTGGLAGLQLCEVGCTYGYFLRKCRIAKARVAGVEWDELALDYLRGM
                     GIPAHKSMPDDAVFDVVCAFQLIEHLADPSGFVAEVARRLAPDGRLLLALPNGSEADR
                     IGPSWVGFRLDLEHFNYFSTRTLAALLARHDLYLEQVWEYMQADLPRADARPVRRGRA
                     RRLIASVWNRLGQGPYHLDGTYILCALARKTSRS"
     gene            complement(5369..>6276)
                     /locus_tag="PKK20_08400"
     CDS             complement(5369..>6276)
                     /locus_tag="PKK20_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99947.1"
                     /translation="ALRIAALSLFLNGISGAQTGALAGLEAFRTIAGLNFITGVVSFP
                     LLVAGALWRGIEGSIGAMAATAAVGYVVNHFALRRELARAGIPQGEPWQRADLPLLWS
                     FALPSGLAMLMVGPVHWACCAILMNQPGGSGEMGAFNAANQWYTALMFLPALLGQSVL
                     PMMAEQLGAAGKRQSLQLLRLSMKLNACTALPVVVLCLASPWIMALFGPNFAHEWPTL
                     VAVLVTAALLAVQIPVGQMIIASGNIWPGFWMNVGWAIVCLAGSWALAGWGALGLATA
                     RLVAYTAHAVWTIWYAFRLLRTESP"
BASE COUNT         1266 a         2090 c         1925 g          995 t
ORIGIN      
        1 accctgcttg aacgcgagac cgccctccgc cagcaggcct aacccggata ttctgcacag
       61 cccgtccgtg caggggacgg tcagatatcg caagaggact gcgatgcgga tcagaacaag
      121 cgccggtggg ggcgtcagcg cgctgtctca acccgtaaat ctcaccagtg ggacgatcgc
      181 ggcattccgg acggtctgcg ggtgagattt ccgggtcagt aaaacccgcg cccagcaagg
      241 agaatcggct catcggttca tcaaagccgg gatgcgaccc agccgcttgg acgcgtcggc
      301 cacgccaaca cagtgtggtc gagattgggt ccaactgttt ggggcgcgag tttctgagag
      361 cccgcatttc aagtgaaatg cgacggtgac cggtcagatg acatcaacag ttggtgggaa
      421 atgcgggctc aagggccaga ggcgcggacc gttgtcacga ccagactcaa gccgcgcacg
      481 cacatctcaa cgtcaaacac ccccggcacc tcccaaccga ggttcatccc ggtcaaacaa
      541 tagtcggcca ggtctcgcga ttcatcgaga aacccgcgcc cccaggccgt ctccaacgcc
      601 tcgactaaca gcgggcgcag gtcgcggtcc acctgaatcc actccccatc atgtgcgctg
      661 cgccgagtga acgactcgcc cggcggtgtg tagatgaaca tgccacttcc cgccgtgtcg
      721 cattgcttgt gctcgggcgg gatgcgcgcc cgatcgtcgt agagcgaaat gccgaaccag
      781 aggaaccgac cgaagcccgg cgaacgccga ttcgagttct gcaaaatgag gaacatctga
      841 tactgcgcgg cgtgcagctc gggcgtgtaa tcgtgtgtgc ggtgcaggcg ggagttgacc
      901 aggcgggcct cgatccgcag tcgggctgtc ccgatctccc cgagcgaggg cgaattcgtg
      961 aagttctgtt gcaccagcaa gtggacccac ggctcaccga gcttcctggc tcccgccaca
     1021 tattcccggc tcgcgttcac agccagcgat agatcggccc gattcctccc gcctccgccc
     1081 acggtaacac cctttgccgc gttggaccac cgcagaactc catgctccga ttcgaccgca
     1141 ggcccggcgg cgagtggaaa ccggctgctc cactgcgcga gatcccagac cggatcagcc
     1201 ttgctcccct tgggcgcaag gcgtccgcac accacacgcc ggcccggcgt cggattcagc
     1261 aggtgaaagc ccaggctgaa gtgcggatcg cgcaccaact cgagtccggg catcacggac
     1321 gcctcgacct ctgcgccgcg acagacgcag atgagcccgc ccaacaagac cgccacgaga
     1381 aagccggagc cttcggcacc agcggaacga aacaccgatt ccgaaatcat ggcccttggc
     1441 tatcagacac cggtcgtgaa gatcaagacc gcgctcggcc ctcaacattg atttgacgcc
     1501 tcgtcctgcg cggggtaagg gatggctatg acattgcatc atctttctca acgcgatttc
     1561 ctgaaactca cgggcacggc gaccttggct gccaccagcg ggctcccagt cggccttctg
     1621 gcggccgacg cgaagaagat acccgtcgga ctccagctct attcggtgcg cgaacagtgc
     1681 caaaaggaca tcgtcggcac ggtcaaggcc gtggccaaga tgggatacaa gggcgtcgag
     1741 ttcgccggtt actacgggcg cgacgcgaag accctgcgcc agttgctcga tgacacgggg
     1801 ctcaagtgct gcggcacgca catcggactc gagacgctgc tcggcgatga actgtccaag
     1861 acaatcgagt tcaatcgggt cctgggaaac ccgtatctca ttgtcccggg cctgggagag
     1921 aaatacacca agacccggac tgcctggcag gagacagccg agatcttcaa cgaaatcgcc
     1981 gagaaggtca aaccccacgg aatgcgcgtc ggctaccaca accacaccac cgaattccac
     2041 gaactcgatg gcgagatgcc gtgggacacc ttttttggcc ggacccgcaa ggacgtgatc
     2101 atgcagttcg acaccggcaa cgcgatgcac ggcggcggcg acgccacgat ctatctcaag
     2161 aagtatcccg ggcgcgccgc gacggtgcac atcaagccgt tctcgaaggc caaacccaac
     2221 gccttgctgg gcgaagacga actgccatgg ccggagatct tccgcctgtg cgagaccgtt
     2281 ggcgccaccg aatggtacat catcgaatac gagagcgatg ccttcccgcc gctcgtaagc
     2341 gtcgagaaat gcctcgaagt gatgcgcaag tggggcaagt gttagcaagg cttcgcccgc
     2401 ccgtgaacct cggaatgctc tcctgattcc caaggatttc cgcattcgag gttcatgggc
     2461 agaatgaatc cgcagtttgg cgggttgggg tctcagatcg acaaggcttg gcgcccgttt
     2521 gcgtgggttg gactccggcg atggtccctg cactccggcg ccttgtcgag tggcgccgct
     2581 gagcggtgct ccggaatagt gcatctggta gtagttggaa ctgttggccg atccactggt
     2641 atttgtgcgg ctgtcggact cggaccctca gccccttgag aatggaaaca ggcggatcgg
     2701 cgcaacagtt cgttgtggag ctgacgattg gtcgagttga agcggacgga ctcaagccgc
     2761 ctggttcgcc gcctcgacga cgcgccggat tttgtcggcg tccgtcacgc cgatctcgta
     2821 ctcgggcagg ccgggcacgc ccatgaagac gtgcggattg cggtagatca tccggctggc
     2881 aatcttgctc tggcccgaga gatgcacctc gcgcacgccg gtggcctcga tgagttcgcg
     2941 cacattgccc tcgtgaatgc ccgccccggg catcacgatc aaccgcgcgc cggcccgccg
     3001 caccagctcg gcgatcagtc ccctgccctc gaaagcgctg ccgcgccggc cggaggtcag
     3061 cagacgctcg accccaagcg ccagcacatc gtccagggca cggtcggcat cggcagccat
     3121 gtcgaaagcg cggtgaaatg tcacctgcat cgggcgcgcc gccgcgacca gcgccccggt
     3181 ccgctccaca tccacactgc cctccggagt caacatgcca atcaccaccc cgtccacccc
     3241 ggcgcgccgg cagaactcga tgtcccgcaa catcaccgcg acctcggtat cgtcgtagca
     3301 gaaatccccg ccgcgcggtc gcaccagaac attgagtccg atgtcgagcc gttggcgcgc
     3361 caactcgatg accgcggcgc tcggagtcac gccgccctcg atcagcgcgg cgcataactc
     3421 gactcgggca gccccgcccg cctgggcggc aagagcggag gtgagggaat tggcgcatgc
     3481 ttcgacgagg actgctgtct gcatatcaaa cgattttcga ttctcagcga cgacggaaac
     3541 cgggacgtca gcgcttcctg cacaggaggt gtgttcaggc tttgcggcgc aagacaaagg
     3601 cctggacagc caaatcgtac accaacaaag gccatcgctt ccgccccaac atcctcttca
     3661 caacctgttc cctgaacagc caccacgccc ggcgcatccc gcgcggagtc tccgaacggt
     3721 acggcccgtt actggtgcgc agcaagtccg ccggatgaac ggccgcctcg ggccagaccc
     3781 actcgaagtg tctgagcaac tccgcgcgca aggcccggta tcgctcgggc ccaatctcgt
     3841 gatcgccccg caaatcaagg tcgttggtca gcaccgccaa tccccccggg cgcaccacac
     3901 gcgcaagctc gcgcaccgtc gattcgaaat ccgggatgtg ttctagcacg ctgatgcacg
     3961 tggcgacatc cgcctcgccc tcagacaatg gcaggcgccc ttgctcgatc atgcggaagc
     4021 tgacccgccc gggcgcggcg gacaccagcg ggacggcccg ctcgaggtcc gtccggcaga
     4081 tcgggtcggt gtcggtgcag acgacgtcgt atccgagcgc tcccagggcg aatgggaaaa
     4141 aggtgacgcc gcttccgacg tccacagcga ccgggcgcga cgcggcaggg gcgtgttgaa
     4201 tgaagcgttc gagatgatag tacgcgtacg gatactccca acatcggctc cagtcgtgca
     4261 gcggatctcg aggccatcga tactcagcgc tccgaaaaga cgattcgtgc cggcggaacc
     4321 gctccgcttc ccgttcgagc cggtcaaaca cagcgatcca ctcggcagtg ttgagatcgg
     4381 acagcgctgc ctgtcccgac cgatgaaaag gatgcccgtc ggcgtaggtc atgatcggga
     4441 tgttttgcgc gccagggcgc acaggatata ggtgccgtcg aggtggtacg gaccctgccc
     4501 gaggcggttc cagacgcttg cgatcaggcg cctggcgcgt ccgcgccgga caggccgggc
     4561 gtcggcgcgg ggcaagtcag cctgcatgta ttcccacacc tgttcaaggt agagatcgtg
     4621 ccgcgcaagc agcgcggcca gcgtccgtgt gctgaagtaa ttgaagtgtt cgagatcgag
     4681 tcggaatcca acccagctcg ggccgatgcg gtccgcttcg ctgccattcg gaagcgcgag
     4741 caacagccgc ccatcgggcg ccaaccgccg ggcaacctca gcgacgaatc cgcttggatc
     4801 ggccagatgc tcgatcaact gaaacgcgca gacaacgtcg aagacggcat cgtcgggcat
     4861 ggatttgtga gccgggatgc ccatgccgcg caaatagtcc agcgccagct cgtcccactc
     4921 gaccccggcg acccgcgcct ttgcgatgcg gcatttgcgg aggaaatagc cataggtgca
     4981 gccgacttcg caaagctgca gaccggccag gccgccggtc tgaagcagta tctcgaggaa
     5041 cggattcggt cgttgccacg gaatccaacg ccgccacctc ggctcgggcg gcggacggag
     5101 ccctttgtac tcaccgtagt ttctgtagaa cgcctgcatc tccgactcgc tgggaatatc
     5161 gggcaggtaa tgcatgccac acgcggcaca acaggccaca acgagccgat ccggcctccg
     5221 gcaaatcgga cgcggcgcat cgccgccaca gaccgggcac ccgcggtgga tgaggccgtc
     5281 caagggccac agcgggacac gcccggcttt gacgccgggc ggcaggggcg gcagcaatgg
     5341 aatggcgtca gtcgggttca cgcaagggtc aggggctctc agtgcgcaag agacgaaaag
     5401 cgtaccaaat ggtccacacg gcgtgggcag tgtaggcaac cagccgggcg gtcgcaagcc
     5461 ccagcgcccc ccagcccgcc agggcccaac tgccggccag gcacacgatc gcccagccga
     5521 cattcatcca gaacccgggc cagatgttgc cgctggcaat gatcatctgc ccgacgggaa
     5581 tctgcacggc caggagggcc gcggtcacca agaccgcgac aagcgtcggc cattcgtggg
     5641 cgaaattcgg tccgaagaga gccatgatcc acgggctggc caggcatagc acgaccacgg
     5701 gcagcgcggt gcaggcattc agtttcatcg agagtcgcag caactgcaga ctctgtcgtt
     5761 tgccggcggc ccccagttgt tcggccatca tgggcaggac cgattgcccg agcagcgccg
     5821 ggagaaacat cagagccgtg taccattggt ttgccgcgtt gaacgccccc atctccccgc
     5881 tcccccccgg ctggttcatc aggatcgcac aacaggccca atggaccggc ccgaccatga
     5941 gcatggccag gcccgagggc agggcgaaac tccagagtaa cggaaggtcc gcccgctgcc
     6001 acggttcgcc ctggggtatg ccggcgcggg ccagctcgcg ccgcagggca aagtggttga
     6061 cgacatatcc caccgcggct gtggccgcca tcgccccaat gctgccctcg atcccccgcc
     6121 acagagcccc tgccacaagc agcggaaacg aaaccacacc ggtgatgaag ttcaggcccg
     6181 caatggtccg gaatgcctcg agccccgcca gcgcgcccgt ttgcgcgccg ctgattccat
     6241 tcaggaacaa cgacaacgcg gcgatccgca gcgcag
//
LOCUS       DAOQRO010000200         6269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12499_length_6269_cov_4.587866, whole genome shotgun sequence.
ACCESSION   DAOQRO010000200 DAOQRO010000000
VERSION     DAOQRO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12499_length_6269_cov_4.587866"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKK20_08405"
     CDS             <1..601
                     /locus_tag="PKK20_08405"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNU99948.1"
                     /translation="TFTGATAHIAVALLAFALIAEPSSLRAADPPIRVLLLSGQNNHD
                     WKTTTPKLKAILTNGGHFAVDVTERPEQCTAETLAQYDLIVSDWNAWGDAKVKEWPPA
                     MREAFLGFVSSGKGYVSIHAGSSSFYDWPEYHQIGGLFWNLDETSHGPPHEFTVEFTR
                     DHPITRASSRLKPRMNSGSSPVSIPMPQSSPPVISNRSP"
     gene            complement(793..4092)
                     /locus_tag="PKK20_08410"
     CDS             complement(793..4092)
                     /locus_tag="PKK20_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99949.1"
                     /translation="MTTIQPLPVQLRACLPRNRQSIAMRWGARYLPLLLTAAGVLPAL
                     FEAGAASEGATLAPIGEPGRAIGLGRGDLALNGSPPEGVWFSAIPAGRAIKVGRGNLI
                     LVESPQEGLWSVATDWTNGWPAGWVHAHAAKVWDEGDWIRVSGQLDLAQGRLELLDSY
                     RREGDVVRGVRRFTWRGKSELPRCTLSVRWVVPGATNAKPLLPGIVYYGNPSGARTGA
                     GSVAMHAGVPGDMSMFEEHRYSAPFACVEWAAADSFRSAALHTVPSRVGGGHHADQWW
                     SLGVKALEAWTELTLLSGPCAANGQPSVVKALQHKFLPYPDAWLTLRPGAVIEKTFFI
                     EVCPSVAQGSGFRQPFRTALRLHPPFSLDGLPTFDQIVRDKHRFAVSRFRDRANDPGF
                     EMYPDYVEGTHYVMGWCGQAEAMGSALLWLAPRLGDPAALNRASRAMDLLASAPFNEN
                     GFLLKYTAESETWSEQDHVSQGQAMENFARAILAARELGKRGGASDEDRKGDDSSPAP
                     SSTSSLKAQGRAPQTSSWESFLRRASALHADRILRDDWRPVSTAEAFYVSPLCKASVL
                     FGNADFQRAAVKAAQHYAERHIEMREPYWGGTLDARCEDKEGALAGFQAFLAVYELTR
                     ESRYLDWAAHALDVALTYTVLWDIDLPAGRLHDHALKTRGWTIVSAQNQHLDVYAVLF
                     TPEIWRIGEYLGREDLKRLAAVMFRSCGQLIDAQGSQGEQIQQTNFGQSGDLTDVFRM
                     RGGYSEGWTVFWITAHFLNAAAQFESMGVDLDRIGASIAQTEGSAEVTPAPLYRDPVY
                     DGAADPVLVWNPFRQAWWMLYTQRRAKLDLPGVEWCHGTEIGVAESRDAGMTWAYRGT
                     LTLEASDAEYSFWAPDVIRDDAGRYHLFVSYVPGAASTHRNWGGQRHILHYSSDDLWR
                     WTFERRVPVASDYCIDPTLIRRPDGAWRMWYKDEGHDSKTYAVESRDLDEWVPVKDPG
                     VSKLYGEGPKAFRFGGWYWLIKDPNSGLDVYRSDDLDGWAYQGKILDQPGSRNSDGTI
                     GKHADVVVCSERAYIIYFTHPYTENAPDRNGVSPLSNRHTAIQAAELEVREGRLVCDR
                     DKAFRIRLTPD"
     gene            complement(4348..>6269)
                     /locus_tag="PKK20_08415"
     CDS             complement(4348..>6269)
                     /locus_tag="PKK20_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99950.1"
                     /translation="LGRPLGIQIYKLVKQLDPDRLVLHQDGTFNTAENSDFRNGPINA
                     WQPGSFACDAPFVAHEYLNLSVKQDPRLAAKFSGVMLPPVPIETRDDWLTDAGLDRRW
                     GDACQDAAHALQRHYQKLGLEAARMDPACDGYSFWTIVDVVVKQGDTYSAQGFLDPFW
                     EPKSNGYTPEAFRVFNAPTVLLLVTEPSARIAVAGDRIRADFWVSHYGDQPLKQTRVS
                     WTLRADKSVLAEGVCSGGDVDLGSVRSLARAEFVISDLQQPAHAILDVSLAETGIRNQ
                     WDFWLFPKRGTRTGAGIAVAPALLPQLVPLYSGLVAADDPEAERAALLVSFFGSPDVE
                     PALASGKRVLLINRTTGAPNVSLGWWAMGEQVGTAFARHPALGVFPHDGHLSPLAFRI
                     LKRGLKLPALPGLHPDEMFVVGEGLDSYYLYAAEARIKQGRALMTFGLDLLSGHPEGT
                     CLLDGLIRYAQSDRFDPRGTVSTLASAPAPNGWSRTLEAGDVGYDHAPLNGVQLDVAR
                     AMRGKNELIWETQPVPATAPHHEVFAITWEGGMGYFAEPQGTFALYVDDTKVLDIPAI
                     SESDTAWWNADRTVSLKYERDNSRPEMGRLTLSLPSSSVTPGKPLRLKVVGSDSNSRR
                     WFGVWQTLGGLSPNG"
BASE COUNT         1191 a         2105 c         1904 g         1069 t
ORIGIN      
        1 gacatttacc ggagccacgg cccacatcgc agtcgcattg ttggcgttcg ccctcatcgc
       61 cgagccctcg tcccttcgcg ctgccgaccc gccgattcgc gtcctgctgc tcagcggcca
      121 gaacaaccac gattggaaaa cgaccacccc caaactcaag gccatcctca ctaacggcgg
      181 acacttcgcg gtggacgtga ccgagcggcc cgagcaatgc accgcggaga ccctggcgca
      241 atacgatctg attgtgagcg attggaacgc ctggggcgac gccaaagtca aagagtggcc
      301 ccctgccatg cgggaggcct tcctgggatt cgtcagctcg ggcaaaggct acgtctcgat
      361 ccacgccggc agttcctcgt tctacgactg gcccgagtac caccagatcg gcgggttgtt
      421 ctggaatctc gacgagacca gccacggccc gccgcacgag ttcacggtcg agttcacccg
      481 cgatcatccc atcacccggg cctcgagccg tttaaaacca aggatgaact ctggatcaag
      541 cccggtgtcc atcccgatgc cacagtcgtc gccaccggtg atctccaacc gctcgccgta
      601 acgaccgctc tgggtcaggg acgcggtttc gcccttctgc tcggccatgc cgcggagtac
      661 atggacaccc ccggttttca aaggctcctg cgtcgcggcg cctgctgggc cgccacgggc
      721 aagtgacttc ggcggccagc acccccgtcg cgctcgtttg attcggacga ctgacccgat
      781 ccggcaaccg catcaatccg gcgtcagccg gatgcggaaa gccttgtcgc gatcgcatac
      841 gagtctgcct tcgcggactt cgagttcggc cgcctggatg gcggtatgac gattcgagag
      901 gggcgagacg ccgtttcgat cgggagcgtt ctcggtgtag ggatgtgtga agtagatgat
      961 gtaagcgcgc tcgctgcaca cgacaacatc cgcgtgcttg ccgatcgtgc cgtcgctatt
     1021 ccggctgcca ggctggtcca gaatcttgcc ctggtaagcc cagccgtcca aatcgtcgga
     1081 acggtacacg tccagtccgc tgttggggtc tttgatcagc cagtaccagc ccccgaagcg
     1141 gaaggccttg ggcccctcgc cgtagagctt cgaaacgccc ggatccttga ccggcaccca
     1201 ctcgtcgagg tcgcggcttt cgacggcata ggtcttcgag tcgtgtccct cgtccttgta
     1261 ccacatgcgc caggcgccgt ccggccgtcg aatcagggtg ggatcaatgc agtagtcgga
     1321 cgccaccggg acccgacgct cgaacgtcca gcgccacagg tcatcggaac tgtaatgcag
     1381 aatgtgccgt tgcccgcccc agttccggtg tgtgctggct gcgcccggca cgtagctgac
     1441 gaacaggtgg taccgacccg catcgtcgcg gatcacgtcc ggcgcccaga acgaatactc
     1501 ggcatccgag gcctcgagcg tcagcgttcc ccggtaggcc catgtcatcc ccgcgtcgcg
     1561 cgactccgcc acaccgatct ccgtgccatg acaccattcg acgcccggca gatcgagctt
     1621 ggcccggcgc tgcgtgtaga gcatccacca ggcttgccga aacggattcc acaccaggac
     1681 cggatccgcc gcgccgtcat acacgggatc acgatacaac ggcgcgggcg tcacctcggc
     1741 gctcccttca gtctgggcga tggacgcccc gatccgatcg agatccacac ccatggactc
     1801 aaattgcgcg gcggcattga gaaaatgcgc cgtgatccag aagactgtcc atccttcgct
     1861 gtagccgccc cgcatccgaa agacatccgt caaatcgccg gactggccaa aattggtctg
     1921 ttgaatctgc tcgccctggc tgccctgggc gtcaatgagc tggccgcaac tgcggaacat
     1981 caccgccgcc aaccgtttta gatcctcccg cccgagatac tcgcctatcc gccagatctc
     2041 gggcgtgaac agaaccgcat agacgtccaa atgctggttt tgcgccgaca cgattgtcca
     2101 gccccgggtc ttgagcgcgt gatcgtgcag gcgcccggcc ggcaggtcga tgtcccacaa
     2161 caccgtgtag gtcagggcca cgtccagggc gtgggccgcc cagtcgagat agcgcgactc
     2221 gcgagtgagt tcataaacag ccaggaaggc ctggaacccc gccagtgcgc cttccttgtc
     2281 ttcacaccgg gcgtccaacg tcccgcccca gtacggttcc cgcatctcga tatggcgttc
     2341 ggcatagtgc tgcgccgcct tgaccgccgc ccgctggaag tcggcgttgc caaacagcac
     2401 ggaggccttg cagagcggcg agacatagaa cgcctccgcc gtcgacaccg gccgccaatc
     2461 gtcccgcaga atgcggtcgg cgtgcaacgc gctggcccga cgcaggaacg attcccagga
     2521 actcgtttgc ggcgctctgc cttgcgcttt gaggctgctg gttgacgagg gtgcagggga
     2581 cgagtcgtcc cccttgcggt cttcatcgga ggccccgccg cgcttgccca actcgcgggc
     2641 cgcgagaatc gcccgagcga aattctccat cgcctggccc tgggagacgt ggtcctgctc
     2701 cgaccacgtc tccgattcgg cggtatactt cagcagaaag ccgttctcgt tgaagggggc
     2761 actggccaga aggtccatgg cgcgggaagc ccgattcaaa gcagcgggat cgcccagcct
     2821 gggcgccagc caaagcagtg cgctgcccat cgcttccgcc tgtccgcacc agcccatcac
     2881 ataatgcgtc ccctcgacgt agtccgggta catctcgaag cccggatcgt ttgcccgatc
     2941 ccgaaatcgt gaaaccgcga agcggtgctt gtcccggacg atctggtcga atgtcggcag
     3001 gccgtcgaga ctgaatggcg gatgcagccg cagggcggtt cggaacggtt gtcggaaacc
     3061 cgaaccctgc gcgaccgacg ggcagacctc gataaagaac gtcttctcga tcaccgcgcc
     3121 cggccgcagc gtgagccagg cgtccggata gggaaggaac ttatgctgaa gcgctttgac
     3181 cacgctcggt tggccgttgg cggcgcacgg ccctgagagc aacgtcaatt ccgtccatgc
     3241 ctccagggct ttcacaccca gcgaccacca ttgatcggca tgatgtcctc cgccaacacg
     3301 agaaggcacg gtgtgcaggg ccgcgctgcg gaaggagtcc gccgccgccc actcgacaca
     3361 tgcaaatggc gcgctatagc gatgttcctc gaacatcgac atgtcgcccg gaacacccgc
     3421 gtgcatcgca accgagcccg ctcccgtccg cgccccggac gggttaccgt aataaacaat
     3481 gcccggaagc aacggcttcg cgtttgtcgc accgggaacc acccagcgca ccgacagggt
     3541 gcagcgcggc agctcggatt tgccgcgcca ggtgaaccgc cgcacgccgc gcacgacatc
     3601 cccttctctg cggtacgaat ccagaagctc gagccgaccc tgcgccagat cgagttgccc
     3661 ggacacacga atccaatcac cctcgtccca gaccttcgcg gcgtgggcat gcacccagcc
     3721 cgccggccag ccgttcgtcc agtccgttgc caccgaccac aacccttcct gaggtgactc
     3781 gacaaggatg agattgcccc ggcccacctt gatcgcacga ccagcgggta tggcgctgaa
     3841 ccacacacct tccggcggcg acccgttgag ggcgagatcg ccccggccca gcccgatcgc
     3901 acgaccgggc tcgccgatcg gagccagcgt cgcgccctcg gacgccgcac ccgcttcaaa
     3961 cagcgctggc agcacccccg cggcagtgag aagcaacgga agataacggg caccccagcg
     4021 catggcaatc gattggcgat tgcgtgggag acacgcccgc agttgcaccg gcagaggctg
     4081 gatcgttgtc atgatcaact cctatccgaa acccgtccaa ctgtcgatga acgcattgcg
     4141 tccggaccgc cattgcctcc gcgacccgga cctcaggact ttgtgggaag cgttgacgtt
     4201 caacggttga gtccggatgg ggaccatgag ccgatgtcag gggggcttgt ctcggagggc
     4261 gaatctccgc aggtccctgg tggatcgaga caatcaccgt caccgatcag gacctcttcc
     4321 gcgaccgcgg gatcgccaga cgggttatca gccattagga ctgagaccgc ccaaggtctg
     4381 ccagacgccg aaccagcgcc ggctgttcga gtcgctaccg acaactttca accgcagggg
     4441 tttgcccggg gtgacactcg acgacggcag cgacagggtc agccgcccca tctccggacg
     4501 cgaattgtcg cgttcgtact tcaggctgac cgtccggtcg gcattccacc aggccgtgtc
     4561 ggattcgctg atggcgggaa tgtcgagcac ctttgtgtcg tccacgtaga gggcgaacgt
     4621 tccctggggc tcggcgaagt agcccatgcc accttcccat gtgatcgcga acacctcatg
     4681 gtgaggggca gtggccggca cgggttgggt ttcccaaatc agctcgttct tgccgcgcat
     4741 tgcccgggcc acatcgagct gcacgccgtt gagcggcgca tgatcgtatc cgacatcgcc
     4801 ggcctcgagc gtcctcgacc acccgttcgg cgcgggcgcg ctggccagcg tcgagacggt
     4861 tccccgcgga tcgaagcgat ccgactgcgc ataacggatc aacccatcca gcagacaggt
     4921 cccttcggga tggcccgaga ggaggtccag cccgaaggtc atcaaggcac ggccctgctt
     4981 gatccgcgcc tccgctgcgt agaggtaata gctgtccagg ccttcgccta cgacaaacat
     5041 ctcgtccgga tgcagcccgg gcagcgcagg caacttcagg ccccttttga gaatgcgaaa
     5101 ggcgagcggg gacaaatgac cgtcgtgcgg gaaaacgccc agggcgggat gcctcgcgaa
     5161 ggcggtgccg acctgttcgc ccatcgccca ccaaccgagg ctgacgttgg gggctccggt
     5221 ggtccggttg atcaggagga cgcgtttgcc cgaggccaat gccggctcga catccggcga
     5281 gccgaagaac gaaacgagca gggctgcccg ctccgcctct ggatcgtcgg cggcgaccaa
     5341 ccccgagtac agcggcacca attggggcag gagagctggt gcaaccgcga ttccggcacc
     5401 agtccgcgtg ccgcgtttgg ggaagagcca gaagtcccat tgattgcgaa tccctgtctc
     5461 ggccaacgaa acgtccagga tcgcatgagc tggttgttga agatcggaga tcacgaactc
     5521 ggctcgagcc agcgaccgga cagaaccgag gtccacatca ccgcccgagc acactccctc
     5581 ggccagcact gacttgtccg cgcgcaacgt ccaagaaacc cgtgtctgct tcagaggctg
     5641 gtcgccgtaa tgcgacaccc agaaatcggc ccggatgcgg tcgccggcga ctgcgatacg
     5701 ggcggatggc tcggtaacga gcaggagcac ggtgggcgca ttaaagacac gaaaggcctc
     5761 tggggtgtag ccgttggatt tcggctccca gaagggatcg agaaaccctt gcgcgctgta
     5821 ggtgtctccc tgtttcacca ccacatcgac aatggtccag aaactataac cgtcgcaggc
     5881 ggggtccatg cgggcggctt ccagccctag cttctgataa tgccgctgca aagcgtgcgc
     5941 ggcgtcctgg caggcgtcgc cccagcgccg atcgagcccg gcatctgtca accaatcatc
     6001 gcgggtctcg atcgggacgg gcggcaacat gaccccggaa aacttcgcgg cgagacgcgg
     6061 atcctgtttc accgagaggt tgaggtactc atgggcgaca aagggcgcgt cgcaggcaaa
     6121 actgccgggc tgccaggcgt tgatagggcc attcctgaaa tcggagttct cggctgtgtt
     6181 gaaagtcccg tcctggtgga gaaccagccg gtcgggatcc agttgtttga ctagcttgta
     6241 gatctggatc cccaatgggc gtccgaggg
//
LOCUS       DAOQRO010000201         6259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12531_length_6259_cov_4.434236, whole genome shotgun sequence.
ACCESSION   DAOQRO010000201 DAOQRO010000000
VERSION     DAOQRO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12531_length_6259_cov_4.434236"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="PKK20_08420"
     CDS             <1..1692
                     /locus_tag="PKK20_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HNU99951.1"
                     /translation="NGWEASDLLQAANREFESLAAQCDSFDKELMADLERAGGEKYAK
                     LCALAYRQCFAGGKFVADANGQPLQFSKENHSNGYIGTSDIFYPMSPQFLLFGPSLAK
                     SFLVPFMNYAASERWKLPFAPHDLGTYPHANGQVYGDGERGENNQMPVEESGNLLLLF
                     GAVAHMEGNADFAGLYWPQLERWAAYLKDKGFDPENQLCTDDFAGHLAHNVNLSAKAI
                     CGLGAFAKLCAARGDNAKAGEYLKLAKEFAARWVKEADDGEKFRLAFDRPGTWSQKYN
                     LMWDRILGLDLFPAEVMRKEMDFYKTIQNRYGLPLDNRQTYTKLDWIAWTATLTQNRA
                     DFEALTDPIFLFMNETPDRSPLTDWYQTKTARKVGFTARPVIGGVFAQMLYDNAVWRK
                     HARRDTTKASNWAPMPKAPIVTEAVPTGERTGQPWRYTTSRPGQGWEKPDFDDSAWKQ
                     GQSGFGTRGTPGARVRTEWNSPNIWLRRQFTLPAIAGQDVQFLIHHDEDAEVYLNGVL
                     ALQLSGYTTAYETAPLTPQAKAALEAGPNTIAVHCRQTGGGQYIDVGLVTVKEVK"
     gene            1701..1856
                     /locus_tag="PKK20_08425"
     CDS             1701..1856
                     /locus_tag="PKK20_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99952.1"
                     /translation="MHALSYRLHPSVIEDLGIAAAINLEDGQSLIEAALAPKPCGKGP
                     GRRFSTT"
     gene            2236..3174
                     /locus_tag="PKK20_08430"
     CDS             2236..3174
                     /locus_tag="PKK20_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNU99953.1"
                     /translation="MQTQSLSRRTFLQHTALAGAAALALPSPARAARGDKLHLATNAY
                     SWQVFYQREGRNFAGSLDAGLKDVAASGLNGFEPGVGGTEDLRTLLPLLKKHGLEMRS
                     VYVNSTLHEPAAADRSVEQILATARAVKPFGTRIIVTNPNPLRWGGPENKTDEQLRTQ
                     AAVMNRLGKELAALKITLAYHYHDIELRMAAREFHHMLAGTDPRHVTLCLDAHWMYRG
                     AGNSQVALFDVIELYGNRISEVHLRQSQEGVWSETFGPGDIDYEALSAALIRKKVKPH
                     IVLEIAVEAGTPKTLDPVEAHRRSAEYARRVFEKMA"
     gene            complement(3195..4601)
                     /locus_tag="PKK20_08435"
     CDS             complement(3195..4601)
                     /locus_tag="PKK20_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU99954.1"
                     /translation="MTTRSLHPGLSRRQFLTIAAGALAAPTIIPASALGADGAVAPSE
                     RITLGAVGWGMQGPGNTGAFMNQKDCQVVAACDLDKNHLASAVNAINRKYGNQDCKAY
                     HDYRELMARKDIDTVMLAVPDTWHALTAVAAAESGKDIYGEKPLARTIAEQQAIVRAV
                     QKHGRIWQTGSWQRSVENFHKAVEIVQNGLIGKVTRVEVGLPAGHHDFAGTAPELLKK
                     LEGVAGAPRNLARLTPTTPGWDAAVSAPPPELDYETWLGPSQMEPYVKTRVHMNWRWN
                     YNVGGGQLLDWIGHHCDIAHWGLGFDNTGPVEVEGQGEFPAKDAVWNTCTRYRITCTY
                     GEGVTMIIAGGHGNIRGGTKWIGTDGWVWVDRGGFDCSNEDLKDISRLPESLRKVKAY
                     HSRDHQRNFLDCVKSRKPTITPVEAAHRSAIPGHLGLIAMLVGRKVKWDPKTEVIVGD
                     AEATKLLSREYRPPYSLA"
     gene            complement(4738..6159)
                     /locus_tag="PKK20_08440"
     CDS             complement(4738..6159)
                     /locus_tag="PKK20_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNU99955.1"
                     /translation="MAQQIKIDPVTRIEGHAKITIQLDEGGNVANARFHVTEFRGFER
                     FCEGRPFSEMPGITPRICGICPVSHLLASAKACDQILAVDIPPAAENLRRMMNLGQIV
                     QSHALSFFHLSAPDFLIGWETPPERRNVFGLIAAHPELARGGIRLRQFGQEVIEHLGG
                     RKIHPAWAVPGGVRHGLSEAGREAIRAKIPEAKAVALKGIECFKEVLKTHERELQVFG
                     NFPSLFMGLIAPDGTWEHYDGRLRFMDGGGNTIADHVDPQRYDQFIGEAVEETSYLKS
                     PYYRPLGYPGGIYRVGPLARLNICRQMGTPLADVELTNFKSMGWGAVTSSFLYHYARL
                     IEILASIERIERMIDSPELASKQLRADAGINRREAVGASEAPRGTLFHHYQVDANGLI
                     QRVNLIIATGQNNLAMNRTVTQIARHFIQGPEIPEAMLNRVEAGIRAFDPCLSCSTHA
                     AGQMALDVTLIDAEGRVLDRVAR"
BASE COUNT         1192 a         2041 c         1903 g         1123 t
ORIGIN      
        1 aacggctggg aagcgtccga cctgctgcaa gccgccaacc gggaattcga gagccttgcc
       61 gcccaatgcg acagtttcga caaggaattg atggccgatc tcgaacgcgc cggcggcgag
      121 aagtacgcaa agctgtgcgc gctcgcctat cgccagtgct ttgccggcgg taagttcgtc
      181 gctgacgcca atggccagcc gctgcaattc agcaaagaga accactcgaa tggctacatc
      241 ggcacttccg atatcttcta cccgatgtcc ccgcaattct tgctcttcgg gccgtcgctg
      301 gcgaagtcgt tcctcgtgcc gttcatgaat tacgcggcga gtgagcgctg gaagctcccc
      361 ttcgctcccc atgatctggg cacctacccg cacgccaacg gtcaggtgta cggcgacggc
      421 gagcggggcg agaacaatca gatgccggtc gaggagagcg gcaacctgct cctgctcttc
      481 ggcgccgtcg cccacatgga gggcaacgcg gacttcgccg gcctctactg gccccaactc
      541 gaacggtggg ccgcctacct caaagacaaa ggcttcgatc cggaaaacca gctttgcacc
      601 gatgatttcg ccggacatct cgcccacaac gtcaatctca gcgccaaggc catctgtggc
      661 ttgggtgcct tcgccaaact ctgcgcggcc cgcggcgaca acgccaaggc cggcgaatac
      721 ctgaagctgg ccaaggagtt cgcggcgcgc tgggtcaagg aggccgacga cggcgagaag
      781 ttccggctcg cattcgatcg tcccggcacc tggagccaga agtacaatct catgtgggac
      841 cgcatcctcg gcctcgactt gttccctgcc gaggtcatgc gcaaggaaat ggacttctac
      901 aagacgatcc agaatcgcta cggtttgccg ctcgataatc gtcagaccta taccaagctc
      961 gattggatcg cctggaccgc gaccctcacc cagaaccgcg ccgacttcga ggctctgacc
     1021 gacccgatct tcctcttcat gaacgaaacg ccggatcgct cgcctctcac cgactggtac
     1081 cagaccaaga cggcgcgcaa ggtcggcttc accgcccgcc ccgtgatcgg tggcgtattc
     1141 gctcagatgc tctacgacaa tgccgtctgg agaaaacatg cccgacgcga cacaaccaag
     1201 gcctccaact gggcgcccat gcccaaggcg cccattgtga cggaagccgt gccaaccggc
     1261 gaacggacag gccagccgtg gcgctataca acatcacgtc caggacaagg ttgggagaaa
     1321 ccggacttcg acgattccgc ctggaagcag ggacagagcg gattcggcac gcgcggcact
     1381 ccgggcgccc gggtgcggac cgagtggaac agcccgaaca tttggctgcg acgccagttc
     1441 acgctgccgg ccatcgccgg ccaggacgtg cagttcctca tccaccatga cgaggatgcc
     1501 gaggtctatc tcaacggcgt gctggcgctc caactctccg gctacacgac ggcctacgaa
     1561 accgcgccgc tcacgcccca ggcgaaggct gccctcgaag caggccccaa caccatcgcc
     1621 gtccactgcc gccagaccgg cggtggccag tacatcgacg tcggcttggt cacggtcaaa
     1681 gaagtcaaat aaccgaggat gtgcacgccc tctcgtatcg gctgcaccct tcggtcatcg
     1741 aggaccttgg catcgcggcc gcaatcaacc tggaggatgg ccagagcctg attgaggcgg
     1801 ctttggcccc caagccgtgc gggaaggggc cggggcggcg tttctccacg acgtgaccag
     1861 cgtatacttc gagggggggc gcacaacgcg ctggcggact ttgggtgcaa ccggaatggg
     1921 agcccgacct tcagcccgca gccacgcgcg ccggaagcca gccttgggac tccccccgtc
     1981 gctcccacca accgcaaaat cgccacgagg tgaagctcgg gcgccagagt cggacgatct
     2041 tcctgcagtc gagggcctgg acacaggatg gcctgatccg cacggctgaa tattcgtacg
     2101 tggatcgagc cgcggccggc cccatttcgt gggctccaaa ctgtttactt gatggaaatc
     2161 gcgggtctgc aaaaaggtct gcggaaagtc ttgccaagga ttgccgcgtc agcctcggca
     2221 tgcttgaatt ggcccatgca aacccaatcg ctgtcgcgtc ggacctttct gcagcacacc
     2281 gcgttggctg gcgcagcggc gctggccctt ccgtcgccgg cccgcgcggc ccgtggggac
     2341 aaactccacc tggccaccaa cgcctattcc tggcaggtgt tttaccagcg tgaggggcgc
     2401 aactttgccg gctcgctgga cgcgggtttg aaggacgtgg cggcgagcgg cctcaatgga
     2461 tttgaaccgg gtgtcggggg caccgaagac ctcaggacgc tgctcccctt gctcaagaag
     2521 cacgggctcg agatgcgatc ggtctatgtc aatagcaccc tgcacgagcc ggcggcggca
     2581 gaccgcagcg tcgagcaaat cctggcgacg gcccgtgccg tgaaaccgtt cggcacccgg
     2641 atcatcgtca ccaatcccaa cccgctccgc tggggggggc ccgagaacaa gaccgatgag
     2701 caactgcgca cccaggccgc ggtcatgaac cgattgggca aggaactcgc cgcactcaaa
     2761 atcacgctcg cctatcatta ccacgacatc gagctgcgca tggccgcgcg cgagttccat
     2821 cacatgctgg ccggaaccga tccgcgccat gtcacgctct gcctcgacgc ccactggatg
     2881 tatcgcggcg ccggcaactc ccaggtcgcg ctcttcgacg tgatcgaact ctacggcaat
     2941 cggatcagcg aggtccacct acgccagtcg caagagggcg tctggtccga aacgttcggc
     3001 cccggcgaca tcgattacga agcgctgtcg gcggcgctca tccgcaagaa ggtcaaaccc
     3061 cacatcgtcc tcgagattgc cgtcgaggcc ggcacgccca agacgctcga tcccgtcgag
     3121 gctcatcggc gcagtgccga gtacgcccgc cgtgtcttcg agaagatggc ctgacgatct
     3181 ttccgcccat gcgatcatgc cagactgtaa ggcgggcgat attcgcggct gagaagcttc
     3241 gtggcctcgg cgtctccaac gatgacctcg gtcttcggat cccacttgac cttgcgtccg
     3301 acaagcattg cgatcaggcc cagatgaccc ggaatagctg agcggtgcgc ggcttcgacg
     3361 ggcgtgatgg tcggctttct ggacttgacg cagtcgagga aattccgctg gtggtcgcgc
     3421 gagtgatagg ccttgacctt gcggaggctc tccggcagcc gggagatgtc cttgaggtct
     3481 tcgttcgagc agtcgaagcc gccgcgatca acccagaccc aaccatcggt cccgatccat
     3541 ttcgtgccgc cgcggatgtt cccatgtccg ccggcaatga tcatggtcac gccctcgcca
     3601 taggtgcatg tgatgcggta tctggtgcag gtgttccaga cggcgtcctt ggccggaaac
     3661 tccccctgcc cttcaacctc gaccgggccg gtgttgtcga aacccaaacc ccaatgcgcg
     3721 atatcacagt gatgtccaat ccaatcgagc aattgtcccc ctccgacgtt gtagttccaa
     3781 cgccagttca tgtggacgcg cgtcttgacg tacggttcca tctgggaggg accgagccat
     3841 gtctcgtagt cgagttcggg cggaggcgcc gacaccgcgg cgtcccaacc cggggtggtg
     3901 ggagtcaggc gggcaaggtt tcttggcgcg ccggcaaccc cttcgagttt cttaagcagt
     3961 tccggggccg tgccggcgaa gtcatgatgc ccggccggca gcccgacttc gacgcgcgtg
     4021 accttgccga tcaggccgtt ctgcacgatc tcgaccgctt tgtggaagtt ctcgactgac
     4081 cgttgccacg atccggtctg ccaaatgcgc ccgtgcttct gaaccgcgcg cacgatggcc
     4141 tgctgctcgg caatggtccg agccagcggt ttctcgccgt aaatgtcctt gccgctttcg
     4201 gcggcggcca ccgcggtcag cgcgtgccac gtgtcgggca cggccagcat gaccgtatcg
     4261 atgtcttttc gggccatcag ttcgcgataa tcgtggtagg ccttgcagtc ctgattgcca
     4321 tacttccggt tgatcgcgtt gaccgccgaa gccaggtggt tcttgtccaa atcgcaggcg
     4381 gcaacaacct ggcagtcttt ctgattcatg aatgcgcccg tgttgccggg accctgcatg
     4441 ccccagccga cggcgcccag cgtgatgcgc tcggacggcg cgacagcgcc atcggcaccc
     4501 agggcgctgg cgggaatgat cgtgggcgca gcgagcgcgc cggcggcaat cgtgaggaac
     4561 tggcggcggg aaagaccggg atgaagcgaa cgtgttgtca tgagcggcat ggtggaatgc
     4621 cgcagccgcg atttcgagca gaaaaagaac ggcagaaaac cgccttctca tgcaacggac
     4681 ggggtatccc cgccgcgaat gttgcacagg gctcccgcgg tccgatatcc gggcttccta
     4741 ccgcgcgacg cgatcgagaa cgcgcccttc ggcgtcgatc agggtcacgt ccagtgccat
     4801 ttgccccgcg gcgtgcgtcg agcaactcag acacggatca aaggcgcgaa tccccgcctc
     4861 gactcggttg agcatcgcct ccgggatttc gggtccctgg atgaaatggc gggcgatctg
     4921 ggtgacggtg cgattcatgg ccaggttgtt ctggccggtg gcgatgatca ggttgacgcg
     4981 ttggatcagg ccgttggcat cgacctgata atggtggaag agggtgcccc gcggcgcttc
     5041 actcgcccca acggcctccc ggcggttgat cccggcatcg gccctcaact gcttgctcgc
     5101 aagctccggg gagtcaatca tccgctcgat ccgctcgatg ctggcgagaa tctcgatcag
     5161 ccgggcgtaa tggtacagaa acgacgaggt caccgcgccc caccccatgc tcttgaagtt
     5221 cgtcagttcc acgtccgcga gcggcgtgcc catctggcgg cagatgttga ggcgcgccaa
     5281 cggtccaaca cggtaaatgc cacccggata gccgagcgga cggtagtacg gcgatttgag
     5341 gtaggacgtc tcttcgactg cttcgccgat gaactgatcg tagcgctgcg gatcgacatg
     5401 gtcggcgatg gtgttgccgc ccccatccat gaaccgcaat cggccgtcgt agtgctccca
     5461 ggtgccatcc ggcgcgatca ggcccatgaa cagactcggg aaattgccga agacctggag
     5521 ttcacgctcg tgggtcttga gcacttcctt gaaacactcg attcccttga gcgcgacggc
     5581 cttcgcttcg ggaatcttgg cgcgaatcgc ctcccgtccc gcttcgctca gtccatgacg
     5641 cacacctccc ggcacagccc acgccggatg aatcttgcgc cctcccagat gctcgatcac
     5701 ttcctgtccg aattgacgca accggatgcc cccccgcgca agctccgggt gggcggcgat
     5761 caatccaaag acattccgac gctcgggcgg cgtttcccac ccaataagaa agtccggcgc
     5821 gctgaggtgg aagaagctca gcgcgtgcga ctggacaatc tggcccaggt tcatcatccg
     5881 gcgcaggttc tccgcggccg gcgggatgtc caccgccaga atctgatcgc aggccttggc
     5941 cgaggccagc agatggctga ccggacagat gccgcagatg cggggcgtga tgcccggcat
     6001 ttcggagaat ggccgccctt cacaaaaccg ctcgaaaccg cggaattcgg tcacatgaaa
     6061 gcgcgcattg gcgacgttgc cgccctcatc caattggatc gtgatcttgg cgtgaccttc
     6121 gatgcgcgtg acgggatcga tcttaatctg ttgggccata ggagaagaat atgacggtga
     6181 agagtgcccc cctcccatca gccgaagcgg atgttctcgc ccgacaaaag ggatcccttg
     6241 ccggcgatga cgtcgcgga
//
LOCUS       DAOQRO010000202         6240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12589_length_6240_cov_7.263703, whole genome shotgun sequence.
ACCESSION   DAOQRO010000202 DAOQRO010000000
VERSION     DAOQRO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12589_length_6240_cov_7.263703"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1182
                     /locus_tag="PKK20_08445"
     CDS             73..1182
                     /locus_tag="PKK20_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU99956.1"
                     /translation="MNETPPLPPTPPSPPQPPEAGPPERSSSTFEDASSTALSEALRS
                     SFRLLKVLMLGLVLLFLCSGMFVVEPNEVVVKLLFGKPVGVGKEQLLQPGWHWAWPYP
                     INEKVRIRIGQSQTATSTTGWHASTPEMEAMNEEPMAYGYLRPEADGYTLTADGNIIH
                     VRASMKYRITDPLQYAFGFAHVERILTNIVNNAIIYASARYTAEAALYRDKLAFRDTV
                     IQRVAQLTEAANLGITLEPSDVETKTPIDVRGAFEDVNKAEQIRSTEISKALSYRDEL
                     VNRTLGQSNAIVNAGLVSSNQMVAAVAATAQVFKDQLPSYQADPHLFKERFLAARLQR
                     VLTNANVKFTLPEGFQELRLQLSREPERAETTSTP"
     gene            1291..3279
                     /locus_tag="PKK20_08450"
     CDS             1291..3279
                     /locus_tag="PKK20_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNU99957.1"
                     /translation="MQVTSLISRAEAACDHCHDRHDHHDHSGHAHHHEHIPVKLSQTL
                     IGMIFIINSYLLDLLFPGSRVVASFSAMIGAVILGAPIIMTSFRDLSRGSLSINELVS
                     IAVLASFASGDLQSAGNYQTAGIVAFFMLLGEIIETRTAAGARASIESLIQLTPTKAR
                     RLLPGGQEEEVPVKDLKVGDLIRVRPGDNVAADGVVVSGQGSINQATITGESLPADKA
                     PGDQVFAGTQNLTSVLDIRVTSAGEDTALGRVHHLILAAEQTKFPLARIVDQYMVYYT
                     PLVLVIGALVWAFTHSLNRVIAVLVISCPCAFILASPTAMVAALSAAARLGILVKNVA
                     DLELAARINSFIFDKTGTLTTGKLAVSRLAPVGEIKPAELLRYAASAEKYSNHPTAKA
                     LLQLAEEVGVPLIEPQDFSETAGRGVKASVDGSHMVIGRARWLKDNGIQEDVLKSVDL
                     SEAEGFSILFVARDGRFVGWIGLRDEVRPEARDSLAHLLEAGVRRVAMVTGDRQPVAA
                     RVAREIGCEEVLAECLPQNKVDYVRQTKGKGYRVAVVGDGVNDAPALAAGDIGIAMGA
                     AGSEVAIHSATIALMNNDLRRLPFLVKLSRMTRNVINQNFLFGICFVIGGQTLAAFGY
                     INPIVAAIMHNAGSLIVVFNSARLVRQGEDLEPFKKAA"
     gene            3381..4676
                     /gene="hisD"
                     /locus_tag="PKK20_08455"
     CDS             3381..4676
                     /gene="hisD"
                     /locus_tag="PKK20_08455"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNU99958.1"
                     /translation="MNVLRHTARDFALKIHEMARETSLFDPEIEQRTRAILDAVQKDG
                     DAALLTFTRQFDGADLRADQLAMTQADLLAASVRVDETFRKTIGFAAKNIEQFSRRSL
                     RRGWSGRNAQGAKVGEKYDPFQRVGLYVPGGTAPLVSTVLMTVVLARVAGCPEIVVCT
                     PCGRDGTVKPELLVAARMAGATEIYRVGGAHAVAAMALGTRTIRPVQKVFGPGNAYVV
                     AAKRLLFGRVAIDLLPGPSEVLVLADDSANPRYVAADLLAQAEHGSGHERVWLVTPSA
                     RLLRAVQTEVKRQVPGLARREFIERALANSGWLIQVKTLAQGIEVANELAPEHCEIHT
                     RDARKVADRILTAGALFLGPWSPTVLGDYVAGPSHTLPTGGAGRNCAGLTVDQFQRRT
                     SVVEYDRPSLLKSLAAVQAFVAMEGLGAHGRSAEVRLRK"
     gene            4743..5930
                     /gene="hisC"
                     /locus_tag="PKK20_08460"
     CDS             4743..5930
                     /gene="hisC"
                     /locus_tag="PKK20_08460"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNU99959.1"
                     /translation="MKSVRSLVRPLVERLQAYVPGEQPKVQGLIKLNTNENPYPPSPA
                     VLRAVKAGVDARLRLYPNPTSELLRRRLARLHRCTIDNIVVGNGSDELLALATRAFVE
                     PLPDGRGSHAKQPHASTVQFFTPSYSLYPVLADIHGAGCKPVPLGPGFSLPVAGPLGR
                     GGGRFAGSLNPDAALTFVTTPNAPTGRGYTTRELDALCRSLRGVVVLDEAYVDFADEH
                     ALSLALRHRHVLVTRTFSKAYALCFQRVGYAVGHPDLIAALHKIKDSYNVNGLGQLAA
                     LATLDDLPYYRANFRRVRATRTRLCAALRSLGFEVFPSQANFLLARPPGLPAERWLHE
                     LRKSKILVRWFSSPSLRDYLRISVGSETETDALLDAVRAIGTSREKGARCHDVSRSAR
                     GKH"
BASE COUNT         1085 a         2101 c         1957 g         1097 t
ORIGIN      
        1 gcccttcgat ctgttgcggg cccaaccgcg cgtgcccgag tccggcaagt agtcgcactg
       61 gatgagcctg ccatgaacga gacaccgcca ttgcccccca ccccgccgag tccgcctcaa
      121 ccgcctgagg ccggtccccc cgagcggtcc tcgagcacat tcgaggacgc gagttcgacc
      181 gccttgtccg aagcgttgcg aagcagcttc cgccttctga aggtgctgat gctggggttg
      241 gtcctgctgt ttctctgctc gggcatgttc gtggtcgagc ccaatgaggt ggtcgtcaaa
      301 ctcctcttcg gcaagccggt cggagtcggc aaggagcagt tgctccagcc gggctggcac
      361 tgggcctggc cctatcccat caacgaaaag gtccggattc gcatcggcca gagccagacc
      421 gccacctcga ccaccggctg gcatgcctcc acgcccgaga tggaggcgat gaatgaggag
      481 cctatggcct acgggtattt gcgccccgaa gccgacggct acaccctgac cgcggacggc
      541 aacatcatcc acgtccgggc cagcatgaaa taccggatta ccgatcctct ccagtacgcg
      601 ttcggtttcg cgcatgtcga gcgcatcctg accaacatcg tcaacaacgc gatcatctat
      661 gcatccgccc gctacacggc cgaggcggcg ctgtacaggg acaagctcgc gtttcgggac
      721 acggtgatcc aacgcgtcgc ccaactgacc gaggcggcca acctgggaat cacactcgaa
      781 ccgagcgacg tcgagacgaa gaccccgatc gacgtgcgcg gtgcattcga ggatgtgaac
      841 aaggccgagc aaatccgcag caccgagatc agcaaagcct tgagctaccg cgacgaactg
      901 gtcaaccgca cgctcggcca gtccaacgca atcgtcaatg ccggcctggt cagcagcaac
      961 caaatggtcg cggcggtcgc tgccacggcc caggtcttca aagaccaact ccccagttac
     1021 caggccgatc cgcacctgtt caaggagcgg ttcctggccg cccgtctgca acgggtcctc
     1081 accaacgcca acgtcaagtt cacgctgccc gagggcttcc aggaactccg tctgcaattg
     1141 agccgcgagc ccgagagggc cgaaaccacc tccacgccat gagactgttc cttgccgttc
     1201 acgcgaaccg ttcggacgcc gcagccgcgc cgcggacgcc ggcgggaacc gcgcgggttc
     1261 attcctgaag ccttatcctt tcgcaacacc atgcaggtca cctctctcat cagccgcgcc
     1321 gaggcggcgt gcgaccactg ccacgatcgc cacgatcacc acgatcacag cggccacgcg
     1381 caccatcacg agcacatccc ggtgaagctc tcccagacgc tcatcggcat gatcttcatc
     1441 atcaactcgt atctgctcga cctgttgttc ccggggagcc gggtggtggc gagtttcagc
     1501 gccatgatcg gggccgtcat tctcggggcg ccgatcatca tgacctcgtt ccgcgacctg
     1561 agccgcggtt cgctgagcat caacgaactg gtcagcatcg ccgtgctcgc ctcgttcgcc
     1621 agcggcgatc tccagagcgc cggcaactac caaaccgccg gcatcgtcgc cttcttcatg
     1681 ctcctgggcg agatcatcga gacccgcacc gccgccggag cgcgggcttc gatcgaatcc
     1741 ctgatccaat tgactccaac caaggcgcgc cggctcctgc ccggcggcca ggaggaggag
     1801 gtcccggtca aggacctcaa ggtgggcgac ctgatccgcg tgcggccggg agacaacgtg
     1861 gcggccgacg gtgtggtcgt gtcgggccag gggtcgatca accaggccac catcactggc
     1921 gagtctctgc cggccgacaa ggccccgggc gaccaggtgt ttgccggaac ccagaacctc
     1981 accagcgtgc tcgacatccg cgtcaccagc gcaggagagg ataccgcgct cgggcgcgtg
     2041 caccatctca tcctcgccgc cgaacagacc aagttcccgc tggcccggat cgtggaccag
     2101 tacatggtgt attacacgcc gctggtcctt gtgatcggcg ccttggtctg ggcattcacc
     2161 cacagtctga accgtgtgat cgcggtgctg gtgatctcct gcccgtgcgc cttcatcctg
     2221 gcgtcgccca ccgccatggt ggccgccctt tccgccgccg cccggctcgg catcctggtg
     2281 aaaaacgtcg ccgatctcga gttggcggcc cgcatcaatt ccttcatctt cgacaagacg
     2341 ggcacgctca ccacgggcaa attggccgtg agcagactgg ccccggtcgg cgaaatcaag
     2401 ccggccgaac tgttgcgcta cgccgcatcg gccgagaaat acagcaatca cccgacggcc
     2461 aaggccctgc ttcaattggc cgaggaggtc ggcgtgccgc taatcgagcc gcaggatttt
     2521 tccgagaccg ccggacgggg cgtgaaggcg agcgtcgacg gcagccacat ggtcataggt
     2581 cgcgcccggt ggctgaagga caacgggatc caggaggacg tgttgaagtc ggtcgacctg
     2641 agcgaggcgg agggattcag catcctgttt gtggctcggg atggccgttt cgtgggctgg
     2701 atcggattgc gcgacgaggt ccgcccggaa gcccgcgatt ccctcgctca cctgctcgag
     2761 gccggcgtgc gccgggtcgc gatggtcacc ggcgatcgtc agcctgttgc cgcgcgcgtg
     2821 gcccgcgaga tcggatgcga ggaggtcctg gctgaatgcc tcccgcagaa caaggtcgat
     2881 tatgtccgcc agaccaaggg caagggctac cgcgtggcgg tcgtgggcga cggcgtgaat
     2941 gacgccccgg cgctcgccgc gggcgacatc ggcatcgcga tgggcgccgc cggcagcgaa
     3001 gtggccattc acagcgcgac cattgcgctc atgaacaacg acctccggcg cttgcctttc
     3061 ctggtcaagc tctcccgcat gacgcgcaat gtcatcaacc agaacttcct cttcggcatc
     3121 tgcttcgtca tcggcggcca aaccctcgcc gccttcggct acatcaaccc gatcgtcgcc
     3181 gccatcatgc acaacgcagg gtccctcatc gtcgtcttca acagcgcccg cctggtccgg
     3241 cagggcgagg acctcgaacc gttcaagaaa gcagcctaac ggagatttgc aggtttcggc
     3301 agcggattcc ggacggggtc tggcttcgtt gctcctcagg cgtctttgac tttcatcccc
     3361 tcttccatca gcatggttcg atgaatgtgt tgcggcacac ggcccgggat ttcgcgctca
     3421 agattcacga aatggctcgg gagacgagcc tgttcgatcc cgagatcgag cagaggacgc
     3481 gggccattct cgatgcggtg cagaaagacg gcgatgccgc gctcctgacc ttcacacgcc
     3541 aattcgacgg agcggatctg cgggccgacc aactggccat gacccaggcc gatcttctcg
     3601 ccgcttcggt gcgggtcgat gaaaccttcc gaaagaccat cgggttcgcc gccaaaaaca
     3661 tcgaacaatt cagccgacgt tcgctgcgcc ggggctggtc gggccgcaat gcgcaagggg
     3721 ccaaggtcgg cgagaagtac gacccgtttc aacgcgtcgg gctctacgtt cccggaggca
     3781 ccgccccgct ggtctcgact gtcctgatga ccgtggtgct ggcgcgcgtg gccggatgcc
     3841 cggagatcgt ggtctgcacc ccgtgcggac gcgatgggac agtcaaaccc gagttgctgg
     3901 ttgcggcgcg gatggcgggc gccacggaga tttatcgagt aggcggcgcg cacgccgtcg
     3961 ccgccatggc gctcgggacg aggaccattc gccctgtgca gaaggtgttc ggccccggca
     4021 acgcctatgt ggtggcggcc aagcgcctgc tcttcggccg cgtggccatc gacctgctcc
     4081 ccggcccgag cgaagtgctg gtgctggccg atgattcggc gaatccccgc tacgtggccg
     4141 cggacctgct cgcgcaggcc gagcatggtt ctggtcacga gcgcgtttgg ctcgtcacgc
     4201 cttccgcgcg gctcctgcgc gcggtgcaga ccgaggtcaa acgccaggtc ccgggcctgg
     4261 cgcggcgcga gttcatcgag cgggccttgg ccaactcggg ctggctcatc caggtcaaaa
     4321 cccttgccca gggcatcgag gtggccaatg aactggcccc cgagcattgc gagattcaca
     4381 cgcgcgacgc acggaaagtt gccgatcgga ttctcaccgc cggcgccctg ttcctcggtc
     4441 cctggtcgcc gaccgtgctt ggcgactatg tggccggccc cagccatacc ctgccgaccg
     4501 ggggcgcggg gcgaaactgc gcgggtttga ccgtggatca gttccagcgg agaacgagcg
     4561 tcgtcgagta cgatcgccca tcgctcctga aatccctggc cgccgtccag gccttcgtcg
     4621 ccatggaagg actcggcgcg cacggacgat cggccgaggt gcgtttgcgc aagtagactg
     4681 gatgtttcct gccgcaattc ggcgtcctgc gccccgccgt tggccacgtg ttttccgatg
     4741 cgatgaaatc tgtgcgttcc ttggttcgcc cactggtcga gcgtcttcag gcctatgtgc
     4801 cgggcgaaca gcccaaagtc cagggcctga tcaagctcaa caccaacgag aacccctacc
     4861 cgccctcgcc cgcggtcctg cgcgcggtga aagcgggcgt tgatgcgcgc ctgcggctct
     4921 atccgaaccc gacctcggag cttctgcgtc ggcggctcgc ccggctgcac cgctgcacga
     4981 tcgataacat cgtcgtcggc aacggctcgg acgaactgct ggccctggcg acgcgggcgt
     5041 ttgtcgagcc gctgcccgac ggacgcggtt cgcatgcgaa acagccccac gcgtccacgg
     5101 tgcagttctt cacccccagt tactcgctct acccggtgct ggccgacatc cacggcgcgg
     5161 gctgcaagcc cgtgccgctc ggaccgggct tcagcttgcc ggtcgcgggc ccattggggc
     5221 gcggcggcgg gcgattcgca ggatcgttga atccggacgc ggcattgaca ttcgtcacga
     5281 cgcccaacgc gccgaccggg cggggctaca cgacgcgcga actcgatgcg ctgtgccggt
     5341 cgctccgggg ggtggttgtc cttgatgagg cgtatgtgga tttcgcggat gagcacgcgc
     5401 tctccctggc tctgaggcat cggcatgttc tggtgacgcg gaccttctcg aaggcctacg
     5461 cgctctgttt ccagagggtg ggatatgccg tgggacatcc ggacttgatc gcggccctgc
     5521 acaagatcaa ggacagctac aacgtcaacg gcctgggaca actggcggcg ctcgccaccc
     5581 tcgacgatct gccctactac cgcgccaatt tccgccgtgt ccgggcaacg cgcacgcgcc
     5641 tgtgcgccgc gctgcgcagc ctcggattcg aggtcttccc gagccaggct aacttcctcc
     5701 tggcgcggcc cccggggctt ccagccgagc gttggctcca cgagttgcgc aagagcaaaa
     5761 tcctggtccg gtggttcagt tccccctctc tgcgcgatta cctgcgcatc agcgtcggga
     5821 gcgagacgga gaccgatgcg ctgctcgatg ccgtgcgcgc cataggaacg tccagagaga
     5881 agggcgctcg atgccacgac gtctcgcgtt cggcccgggg gaagcattga cattcagcgg
     5941 tcgaatgcgc acggggacca tgaaccgatt tgtagccgcc gacgtgagtc ggcgctgatt
     6001 tctgcttttg acggagcgag atggcgcgga ctcacgtccg cggctacgga accacggttc
     6061 atgggaaggt tcatctttag cgttgacatc cggcgattgg atgcgcgatg gggatcgtaa
     6121 ccctgtcgca gaggcaccgc tccgggaatg gcggagtcta actgataaag aacctgtcac
     6181 tttatccgtc cccacaccgt gggtgagggc taaagtcgcg gcggatgtcc agagggcctg
//
LOCUS       DAOQRO010000203         6175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12761_length_6175_cov_5.739379, whole genome shotgun sequence.
ACCESSION   DAOQRO010000203 DAOQRO010000000
VERSION     DAOQRO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12761_length_6175_cov_5.739379"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="PKK20_08465"
     CDS             <1..1894
                     /locus_tag="PKK20_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNU99960.1"
                     /translation="VQIACGSRFYRGAFNQLRVGKANMDMLVALGSTTAFVYSTWGLF
                     AGAPTHLYFMEAVGILTLISLGHWLEAAATARASAALRSLIELAPPKARRLGPDGAES
                     AVPVATLVIGDRILLAPGDRVPTDGEVIDGESALDESMLTGESMPVEKRAGAKVYAGT
                     INGSGRLVARVTAIGEDTALANIIAVVQRAQNSRAMIQKLGDRVSAVFVPAVVLVAIA
                     TGLWWGLDYASAQETAGRVMAWLWAVTLPATPLAAAFIHAAAVLIVACPCAMGLATPA
                     AIMAGANAAARRGLLIRDGAALEKSGRITAVLFDKTGTLTQGKVSVAAFADLRAGSGR
                     DGTAQALAADLAEPSRHPLSVAIAAWGRIRPQARTVGLAKAPALENWREVRGCGVEAR
                     RDGAVVRLGSLRWLREGGVDVTPATRFIAEWTERGATVLGVAKGGALDGMMALTDTLK
                     AGAEDVVAAIRSQGKEVYLVTGDQAATAAAIARQAGIDPTRVFAEVPPERKAEIVQRL
                     QEQGQRVAFVGDGINDAPALEQADLGIAVSRASDVAREAADIILLRSDIEAIPEAMGL
                     ARATLRTIRQNLFWAFFYNAAAVPLAALGFLSPLVCAAAMGLSDVLVIGNALRLRRWR
                     YRNWNP"
     gene            complement(1955..4870)
                     /locus_tag="PKK20_08470"
     CDS             complement(1955..4870)
                     /locus_tag="PKK20_08470"
                     /inference="COORDINATES: protein motif:HMM:NF009948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional fucokinase/fucose-1-phosphate
                     guanylyltransferase"
                     /protein_id="HNU99961.1"
                     /translation="MNATRLPIQVLLSLPSRMAEAFEALEGRPRPEWFATCDPPGPHI
                     GSGGGTAHVLAEAWRATAPASPFTEWLGQSRKLIIHGGGQSRRLPAYAPVGKLLMPIP
                     VFRWARGQRLDQTLLDLQLPAYRRVLDHAPSGCVAMVASGDVLLEFARQLPPFPAVDV
                     LGLGMWVPPEKARYHGVFFANRRRPTELAFFLQKPSLAQIREAAGESFPLVDTGMWLL
                     SERAIRVLMQRCGWDGGGFAGDRASNYELYSRFGLSLGSSPVEPNPEISALTCAVVAL
                     PEARFHHFGTSRQMIESISTLQNAQLDETRLGITGVKRHPDIYRLNCRFDLPIGLDKN
                     HTLWVENSVVPRTWNLGSEHVLTGIPDNAWDLRLESGVCLDFVPVSDSAFAVRFYGIN
                     DEFRGPVGNPLTLWLGAPAPDWFARRRIDLGAAGLDPNADVQQAPLFPVLGPEALAPR
                     FIEWLCAPDPVPKPEFATLWREARRLSAEGLSQQVNLERLYRQRAQRRRQCLLPMLRN
                     SRWSVFYRLDLEATARMFAETSDPLPEQPSNADAAQPVEPLHRVHDEMFRSSVMRHRR
                     QRDWEQLEQSAFARLRELIVQEVQLQPVLPRCNVLEDQIIWGRSPVRLDLAGGWTDTP
                     PYCLEFGGQVVNVAVDLNGQPPIQVFCKLCPRPEIVLRSIDLGVEQRIRRYEDLDTCG
                     QPGSEFGLAKAALALVGFLPRFHAHGRFATLEEQLLDFGGGIEISMLAAVPKGSGLGT
                     SSILAATLLATVGELCGLGWDRDVLSSRTLALEQMLTTGGGWQDQAGAIFRGLKLVET
                     TPGLAQKPRVRWLPHHLFEPAYANTLMLLYYTGVTRLAKGILHEIVRGIFLNSPEHLA
                     TIGDIGANAARACDALQQCDYAALGEVIRRSWQLNQRLDAGTNPPETQRILDQVGDWL
                     AAAKLLGAGGGGFFLMLAKDEEAAKRVRRSLIESPPNSRARFVNLSLSETGLQLTRS"
     gene            5004..5651
                     /locus_tag="PKK20_08475"
     CDS             5004..5651
                     /locus_tag="PKK20_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNU99962.1"
                     /translation="MKSSTQLEYYFVQSLTLIRVPLILAFLAISVFAGVPLSAGWFLA
                     ALGAMILSAITDLFDGYFARRFGVTSRLGSHADPLTDKVFYLTTFPTLVYLAARDGQL
                     GHARILLVLAILFLLRDQWVSFLRSIGALHDMSAKANWSGKVRTLISFPTICLIYYFL
                     QAPADWTLRPPVWLVHGLEGLSMLINLISIGVYTRRFLPALRAELRPDQQSGLSR"
     gene            complement(5921..>6175)
                     /locus_tag="PKK20_08480"
     CDS             complement(5921..>6175)
                     /locus_tag="PKK20_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cycloartenol synthase"
                     /protein_id="HNU99963.1"
                     /translation="GMGRQGYYYYLHLMAKALNACGMDALELKDGRQIAWRRELALRL
                     MNLQNADGSWVNEENRWWEKDPHLVSAYAVLALDILWRGL"
BASE COUNT         1033 a         1877 c         2167 g         1098 t
ORIGIN      
        1 ggttcagatc gcttgcggat cgcgattcta ccggggcgcg ttcaaccagc tgcgggttgg
       61 aaaggcgaac atggacatgc tggtggcttt gggctcgacc acggcgttcg tctacagcac
      121 ctggggcctg ttcgcaggcg ccccaacgca tctgtacttc atggaggcgg tgggcatcct
      181 gaccctgatc agcctcggac attggctcga agccgccgcc acggcgcgag cgtccgccgc
      241 ccttcgcagc ctcatcgagt tggcgccgcc caaggcccgg cgccttggcc cggacggcgc
      301 ggagtcggct gtcccggtcg ccacattggt gatcggagac cgcattctgc ttgcaccagg
      361 cgaccgcgtc ccgaccgacg gcgaggttat cgacggcgag tcggcactcg acgagtcgat
      421 gctgaccggc gagtcgatgc cggtcgagaa gagggcgggg gcgaaggtct acgccggcac
      481 gatcaacggc agcgggcgat tggtggcgcg agtgacggcg attggcgaag acaccgctct
      541 ggcgaacatc atcgcggtcg tccagcgggc gcagaacagc cgggccatga ttcagaagct
      601 cggagaccgg gtcagcgccg tgtttgtgcc ggccgtggtg ctggtcgcga tcgcgacggg
      661 tttgtggtgg ggactcgatt acgcgtcggc ccaggagact gccgggcggg tcatggcctg
      721 gttatgggcg gtcactctgc cagcgacgcc cctggccgcg gcattcatcc atgccgcggc
      781 ggtgttgatt gtcgcctgtc cctgcgcaat gggcctggcg actccggccg ccatcatggc
      841 cggcgccaat gccgcagcgc ggcgcggtct gctgatccgc gacggcgcgg cgctcgagaa
      901 gagcggacgc atcacggccg tgctgttcga caagaccggc acactgaccc agggcaaggt
      961 gagcgtggct gcgtttgcgg atttgagagc gggctcggga cgggatggaa ccgcgcaggc
     1021 gctggcagcc gatctggccg agccgtcgcg gcacccgctc agcgtggcaa ttgcggcgtg
     1081 ggggcgcatc cggccccagg cgaggacggt cggattggcc aaggcgcccg ctctcgagaa
     1141 ttggcgcgaa gtccgcggat gcggagtcga ggcgcgccgg gacggagccg ttgttcgact
     1201 gggatcgctt cgctggctgc gcgagggcgg tgttgatgtc acgccggcaa cgcgattcat
     1261 tgccgagtgg acggagcgag gcgcgaccgt gttgggggtg gcgaagggcg gcgctttgga
     1321 cgggatgatg gcattgacgg acacgctgaa ggccggagcg gaggacgtgg tggcagcgat
     1381 tcgttcccag ggaaaggaag tgtatctggt gacaggcgat caggcggcga cggccgcggc
     1441 catcgcgcga caggctggga tcgatccaac acgcgttttt gccgaggtcc cgcccgagcg
     1501 gaaggcggag attgtgcagc gtttgcagga gcagggacag agggtggcgt ttgttggcga
     1561 cggaatcaac gacgccccgg cgctcgaaca ggcggacctg gggatcgcag tcagccgagc
     1621 cagcgatgtt gcgcgcgagg cggccgacat catcctgctg cggtcggaca tcgaggcgat
     1681 tccggaggcg atgggactgg cgcgggcgac gcttcgcacg atccggcaga atctgttttg
     1741 ggcctttttc tacaatgcgg cggcggtgcc gctggcggca ctcgggtttc tcagtccgct
     1801 ggtttgcgcg gcggcgatgg gtctatcgga tgttctggta atcggcaacg cgctgcgtct
     1861 gcggcgttgg cgatatcgca actggaatcc ttgatccctt caacgcagct cagaaacttg
     1921 atccgtctgc aaggcgtttg accggtcgag gattctaact gcgcgtcaac tggaggccgg
     1981 tctcggagag gctcaggttt acgaaccgcg cccgcgagtt gggcggggac tcgatcaagg
     2041 agcggcggac gcgcttggcg gcttcctcgt ctttggcgag catgaggaag aagccgccgc
     2101 cgccggcgcc caggagtttg gcggccgcca gccagtcgcc aacctggtcg aggatgcgct
     2161 gggtttcggg cgggttggtg ccggcatcga gccgctggtt caactgccaa ctgcggcgga
     2221 tgacctcgcc gagcgcggca taatcgcatt gctggagggc atcgcaggcg cgcgccgcgt
     2281 tggcgccgat atcgccaatg gtggccaggt gttccggcga attcaggaag atgccgcgca
     2341 cgatctcgtg gaggatgcct ttggcgagcc gggtcacacc ggtgtaatag agcagcatca
     2401 gcgtgttggc gtaggcgggc tcgaacaaat gatgcgggag ccaccgcact ctcggcttct
     2461 gcgccaggcc cggcgtggtc tcgacgagtt tgagtccgcg gaagatggcc ccagcctgat
     2521 cctgccagcc gccgcccgtg gtaagcattt gctcgagggc gagcgttctc gaggacagga
     2581 cgtcgcggtc ccacccaagt ccgcacagtt cgccgactgt tgcgagcagg gtggcggcga
     2641 ggatgctgct ggtgcccagc ccggagcctt tggggacagc ggcgagcatc gagatctcga
     2701 tgccaccgcc aaaatcgagc agttgctctt ccaaggttgc gaatcggccg tgggcgtgga
     2761 agcgggggag gaatcccacg agggccaagg cggctttggc gagtccgaat tcgctccccg
     2821 gttggccgca ggtgtcgaga tcttcgtagc ggcggattcg ttgctcgacg cccaggtcga
     2881 tcgagcgcag gacgatttca ggccgcggac acagcttgca gaagacctgg atcggcggct
     2941 gtccgttgag gtccaccgcc acattcacca cctggcctcc gaattccaga cagtaaggcg
     3001 gggtgtcggt ccagcccccc gcgagatcga gccgcaccgg gctgcgtccc cagatgatct
     3061 ggtcttcgag gacgttgcat cgtgggagca caggctgcaa ctgaacctcc tgaacgatca
     3121 actcgcgaag ccgggcgaaa gcgctctgtt cgagttgttc ccagtcgcgt tgccggcgat
     3181 ggcgcatcac ggacgatcga aacatctcgt cgtggacgcg atggagcggt tcgaccggct
     3241 gggccgcatc ggcattcgaa ggctgttctg ggagcgggtc ggaggtctcg gcgaacatcc
     3301 tggccgttgc ttcgaggtcg agccggtaaa agacgctcca gcgcgagttg cgcagcatgg
     3361 ggagcaggca ttggcggcgg cgctgggccc gctgccggta gaggcgctcg aggttgacct
     3421 gctggctgag gccttcagcc gagagccgcc gcgcctcgcg ccagagagtc gcgaattcgg
     3481 gcttgggcac ggggtcgggt gcgcagagcc actcgatgaa gcgcggggcc agcgcctcgg
     3541 gtccgaggac ggggaacagg ggcgcctgct ggacatcggc gtttggatcg aggcctgccg
     3601 cgcccaaatc gattcgtcgg cgggcgaacc aatcgggcgc cggggcgccc aaccagaggg
     3661 tcagcggatt cccgaccggg ccgcggaact cgtcgttgat tccatagaac cgcactgcga
     3721 atgcgctgtc gctcaccggc acgaaatcca gacagacccc ggactcgagc cgcagatccc
     3781 aggcgttgtc ggggatgccg gtcaacacgt gctcggagcc gaggttccac gtccgcggca
     3841 cgacgctgtt ctcgacccag agggtgtggt tcttgtcgag accgatcggc aggtcgaatc
     3901 ggcagttgag gcggtagatg tccggatgcc gtttgacgcc ggtgatgccc agccgggtct
     3961 cgtccaactg ggcgttttgc agcgtcgaaa tcgactcgat catctggcgg cttgtgccga
     4021 agtggtggaa tcgcgcctcg gggagcgcca ccacggcgca ggtcagggcg ctgatctcgg
     4081 ggttgggctc gaccggggaa ctgcccagcg acaaaccgaa ccggctgtag agttcgtagt
     4141 tcgaggcgcg gtcgccagca aagccgccgc catcccagcc gcaccgttgc atgagaaccc
     4201 ggatcgcgcg ttcgctcagc agccacatgc ccgtgtcgac caaggggaaa gactcgcccg
     4261 cggcctcgcg gatttgggct aggctgggct tctgcagaaa gaacgcgagt tctgtcgggc
     4321 ggcgccggtt cgcgaagaag accccgtgat agcgggcctt ctcgggcggc acccacatgc
     4381 ccaggcccag gacgtccacg gcaggaaacg gggggagttg gcgggcaaat tcgagcagca
     4441 catcgccgct cgcgaccatt gccacgcatc cggagggcgc gtggtccaac acccgccggt
     4501 aagccggcag ttgcagatcg agcagggttt gatcgagtct ctgtccgcgc gcccagcgga
     4561 acacggggat cggcatgaga agtttgccca ccggcgcata ggcaggcagc ctccggctct
     4621 gaccgccgcc gtgaatgatg agcttgcggc tttggccgag ccattcagtg aagggagacg
     4681 ccggcgcggt tgcgcgccag gcctcggcca gcacgtgggc ggtgcctcct ccggatccga
     4741 tgtgcggacc gggcgggtca cacgtggcaa accactcggg gcgcgggcgt ccctcgaggg
     4801 cttcaaacgc ctccgccatg cgcgacggaa gcgacaacaa gacctgtatc ggaagacgag
     4861 ttgcgttcat gcgaccggtg caatcgtgtc atccgttcgt gggcgcgtcg agccgattgc
     4921 agtccggcgc gggtgccaac ggctggatgt gaagacaagg tggtgctttc cgtcgggcaa
     4981 gtttgccatt agggtgcggc gaaatgaaat cctcgacgca gctcgagtat tattttgtcc
     5041 agtcgctcac gctcatccgt gtcccgttga tcctcgcttt cctggcgatc agcgtgttcg
     5101 ccggtgtccc actttccgcc gggtggtttc tcgcggcctt gggggcaatg attctgtcgg
     5161 cgatcaccga tctgttcgat ggctactttg cgcgccgatt cggggtgacc tcccgtttgg
     5221 gcagtcacgc cgatcctctg acggacaagg tcttctacct gacaacgttt ccgactctgg
     5281 tctacctggc ggcccgagac ggacaactcg ggcacgcgcg catcctcctg gtgctcgcga
     5341 tcctgtttct gctgcgggat cagtgggtct cgttcctccg ctcgatcggc gcgctccacg
     5401 acatgagcgc caaggcgaat tggtcgggca aggtgcggac gctcatctcg tttccgacga
     5461 tctgcctgat ttattacttt ctgcaggctc cagcggattg gaccttgcgc cctcccgtgt
     5521 ggttggtgca cggcctcgaa ggtttaagca tgctgatcaa cctcatctcg atcggcgtct
     5581 atacgcggcg gttcctgccc gccctgcgcg ccgaattgcg gcccgatcag caatcaggcc
     5641 tgtcgcgata ggggcacgcg aacacgctgt tcaggcccgc atttcgtagc gcggcacggc
     5701 cgcaaccaaa agtcggaggg ccgatccggc ggcggcgcat ccgcggtaca ggccccgtct
     5761 cgagacaatc accgtcaccg accagggcct ttcgggcgac tgcgggatca gccatcagag
     5821 atacgtcgac ggctgggcag atttcgggtg atagcacaac acccattcag tgcacggtgc
     5881 agtcagacgt cgcaggacga ctgcaatgcg ggttagaacc tcacaaaccg cgccacagga
     5941 tgtccagggc aagcacggca tacgcgctga cgaggtgggg atccttctcc caccagcggt
     6001 tttcctcatt gacccacgac ccgtcggcgt tctgcaggtt catcaggcgc agcgcaagct
     6061 cgcggcgcca cgcaatctgg cgtccgtcct tcagttcgag tgcatccatg ccgcaggcgt
     6121 tgagggcttt ggccatcaga tgcaggtagt agtagtatcc ctgccgtccc atgcc
//
LOCUS       DAOQRO010000204         6174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12767_length_6174_cov_4.361497, whole genome shotgun sequence.
ACCESSION   DAOQRO010000204 DAOQRO010000000
VERSION     DAOQRO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12767_length_6174_cov_4.361497"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PKK20_08485"
     CDS             <1..1364
                     /locus_tag="PKK20_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNU99964.1"
                     /translation="IIHVLISHVAVGGGAFLVVAEIWSRRQPDGQRVRDWLHTFATFF
                     LVYTTVLGATTGVGIWFSVQLASAEAISLLIHQFVFAWAIEWVFFLSELTVLYLYYYG
                     FKRNTPRMQTFLAGAYFVIAWGSLVVINGILTFMLTPGAWTIENKDIAAGFFNPGYFP
                     ALLIRTVTMFLLAGLAGFLVASRIRDEEFKGRIIQFCVKWVIPGAILVPGLGYWYFTT
                     LPASTVTILAGGTVGVVGGTLSALTRMAILAGVAGTSLVLGTLVFAAYPKTVNTIAAI
                     SMLLVAQLGIMGAEFFREMGRKPYVIHGVLYSNGLWVKDAKDPARLNGPYLEKAKWHG
                     GVASGTLAHGEQVFRLQCASCHTRDGYRSVVYRTRNWTAGFGSRWLGTMHDMGVMPPF
                     QGDALDKAALVAYVLSLRGETVDPRDLLPDRQATAQGDDLDPDGSGQALPGLEVAGAE
                     VGR"
     gene            1361..2533
                     /locus_tag="PKK20_08490"
     CDS             1361..2533
                     /locus_tag="PKK20_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99965.1"
                     /translation="MRLALAMAGVLAAATASGAGLPMPSPDPLGYPVPPILLQALSYF
                     TLTLHFLAMNLTVGSVVIYLWLTITRKQYDDRLARYLRDLLPLSTSYLITFGIPPLLF
                     VQVLYGQMFYSSSILLGFPWILVIPLVVFLYGAFYYQKLVKNPEHPGVLLLSVLAAMA
                     LLSVGFFFVNNITLSVSPEKWMGLYAKSPGGQNLNMDDPTLFARWLLILAPGFTVAGV
                     AILFRGVMMLKWGLTEEGRHNHRIAVNAAVLGMLLEAVAGIWILSAMPDDLRSHVTSA
                     GLAKLLLGLGTAMASVGLVLVFLGRYKASWAYPLMASLAFFLALAPLVALRDVVRIEQ
                     LRPHFSLENVPVHAQWCMAGLFAVFLVAGLVFLLALHMMVVPKLIAGRRAELRESA"
     gene            complement(2550..2714)
                     /locus_tag="PKK20_08495"
     CDS             complement(2550..2714)
                     /locus_tag="PKK20_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99966.1"
                     /translation="MLVVLSYETPELDYFSARALPFLKDLIDRYYRAGVRLNGLYADA
                     QGRRRHFYLL"
     gene            complement(2920..3153)
                     /locus_tag="PKK20_08500"
     CDS             complement(2920..3153)
                     /locus_tag="PKK20_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNU99967.1"
                     /translation="MADIANKYPENVPGKYYVDNQCIDCDLCRETAPNNFQRNEDGGY
                     SFVCKQPENEDEESQCKDAKEGCPVEAIGDNGA"
     gene            complement(3207..5522)
                     /gene="pnp"
                     /locus_tag="PKK20_08505"
     CDS             complement(3207..5522)
                     /gene="pnp"
                     /locus_tag="PKK20_08505"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNU99968.1"
                     /translation="MPETVTVPVGAGQIHIETGLLAKQADGAVTVRLGETIVIVAAVA
                     AATPREGQEFFPLTVDYREKAAAAGKFPGGYFKREGRPTEKEILTSRMIDRPIRPLFP
                     KGWFNEVQVQSILLSADGENDPDILSINGASAALMVSDIPWAGPIAAVRVGRIKGQFV
                     VNPTHAQKAESDLDLVYVGSETEMVMFEGSGLEIPEADFIGALSFAQLCCQPLISAQK
                     DLAAKAGRPKRSITVNVVPDEILREAKALAGDQSVPALLTPGKHEREAAWKVVTERVA
                     PKLIEKFGAEKITEFVLKDTFNHLQKEAVRDQILNHGKRLDGRAMDEVRPISCSVGLL
                     PRAHGSALFTRGETQALALVTLGTTEDSQEIDAYAGGDKEKQFILHYNFPNFSVGETG
                     RIAGPGRREIGHGALAERSIEPMVPLKEFPYAVRVTSEIMESNGSTSMATVCGGSLAL
                     MDAGVPLVRPVAGVSIGLCTEYGPSGKIARYEMLTDIIGAEDFFCDMDCKIAGTEKGV
                     TGFQLDLKLKGIPHLLMAEAIEKARVARLKILKIMAETIAEPRKELSKYAPRILTLKV
                     NPEKIGALIGPGGKNIKRIVDESGCEINIEDDGTVQIYSTSVEGMEVARREVEAISAE
                     IEVGKLYRGKVVTIKDFGCFVEVFPGQDGLVHISELANFRVKQTEDIVKLGDEIWVKC
                     LGVDEKGRVRLSRKAAMEERDKLEREKQPSGEGERPARRPEGDRPPRSEHRSERPERR
                     ERPNRGERYDRGSRPDRGERPPPEGTHANQA"
     gene            complement(5769..6026)
                     /gene="rpsO"
                     /locus_tag="PKK20_08510"
     CDS             complement(5769..6026)
                     /gene="rpsO"
                     /locus_tag="PKK20_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNU99969.1"
                     /translation="MEAKTKTITDFRLHEKDTGSADVQIALLTERINQLTDHLQKHRK
                     DHSSRRGLLMMVGQRRRLLDYLHTTDSDRYLAVTKKLKLRR"
BASE COUNT         1007 a         1920 c         1880 g         1367 t
ORIGIN      
        1 caatcatcca tgtgctcatc tcgcatgtcg cggtgggcgg aggcgcgttc ctggtggtgg
       61 cggagatttg gtcgaggcga cagcccgacg ggcagcgagt ccgcgactgg ctgcacacgt
      121 ttgccacctt ctttctggtg tacacgacgg ttctgggggc cacgaccggc gttggcatct
      181 ggttctcggt ccaactggcc agcgccgaag ccatcagcct gctgattcat cagttcgttt
      241 tcgcttgggc catcgagtgg gttttcttcc tgagcgaact gaccgtgctc tacctctatt
      301 actacggctt caaacgcaac acgccgcgga tgcagacctt tctcgcaggc gcctacttcg
      361 tcatcgcctg gggctctctg gtggtgatca acggcatcct gacgttcatg ctcaccccgg
      421 gcgcctggac gatcgagaac aaggacatcg cggccggctt cttcaatccc gggtacttcc
      481 cggctttgct gatccgcacg gtgacgatgt tcctgctggc cggcctagcc ggcttcctgg
      541 tggcaagccg catcagggac gaggaattca aggggcggat catccagttc tgcgtgaagt
      601 gggtgattcc tggggcaatc ctggttcccg gcctaggcta ttggtatttc acgaccctgc
      661 cggcaagcac ggttacgatt cttgccgggg gcaccgtggg agtggttggc gggacgctct
      721 cggcgctcac acgcatggcc atcctggccg gggtggcggg gacgtcactg gtcttgggaa
      781 cgctggtgtt cgccgcgtat cccaagacgg tcaacaccat tgcggccatc tcgatgctgt
      841 tggtggctca gttggggatt atgggagcgg agttcttccg cgaaatgggc cgcaaaccgt
      901 atgtgatcca cggggtcctt tactcgaacg gcctttgggt gaaggatgcc aaagacccgg
      961 ctcgactgaa tggtccctac cttgaaaagg ccaagtggca tggcggcgtc gcgtccggca
     1021 cgctcgccca cggggaacag gtctttcgcc tccaatgcgc cagttgccac acgcgcgacg
     1081 gttatcgctc cgtggtctac cggacccgca actggactgc cgggtttggc tcccggtggc
     1141 tgggcacgat gcacgacatg ggggtgatgc cgccgtttca gggggatgcg ttggacaagg
     1201 ctgccctagt ggcttacgtg ctctctctcc gcggcgaaac cgtcgatccg cgcgacctgc
     1261 tgccagatcg gcaggccacg gctcaaggcg atgatctcga tccagacggg agtggccagg
     1321 cgctgcccgg tctcgaagtg gctggagcgg aggtggggcg atgaggctcg cactcgcgat
     1381 ggcgggcgtg ctggccgctg caaccgcgag cggcgccggt cttcccatgc cgagtcccga
     1441 cccgctgggt tatccggtac cgcccatcct gctgcaagcg ctgtcctact tcactctgac
     1501 cctgcacttc ctggcgatga acctgacggt tgggagcgtt gtcatctacc tgtggctgac
     1561 gatcacgagg aagcagtacg acgaccgttt ggcgaggtac ctgcgggatt tgctgccgct
     1621 gagcacctcg tacctgatca cgttcggcat cccaccgctg ctcttcgtcc aggttctgta
     1681 tgggcagatg ttctactcct cgtcgattct gctcgggttt ccctggattc tggtaattcc
     1741 actggtggtc ttcctctacg gggcgttcta ttaccagaag ctggtgaaga acccggagca
     1801 tccgggcgtc ctgctgctct cggttctggc ggcgatggca ctgctgagcg tgggtttctt
     1861 ctttgtcaat aacatcacat tgtcggtctc gcctgagaag tggatggggc tctacgcgaa
     1921 gtcgcctgga ggccagaact tgaacatgga cgacccaacg ctctttgccc ggtggttgct
     1981 gatcctggcg cccggtttca cggtggcggg cgtcgccatc ctgttccggg gcgtcatgat
     2041 gctgaagtgg ggtttgaccg aggagggaag acacaatcac cggattgccg tgaacgcagc
     2101 ggtcctgggc atgctgctcg aagcggttgc cggtatttgg attctgtccg ccatgccgga
     2161 cgacctccgt tcacacgtca catcggcggg tctggccaag ttgcttctgg gtctgggcac
     2221 agccatggcg tccgtcggtc tggtgcttgt cttcctgggg cgatacaaag cctcgtgggc
     2281 ctatccgctc atggcttcac tggcgttctt cctggccctg gcgcccctgg tcgctctgcg
     2341 agacgtcgtg cgtatcgagc agttgaggcc tcacttctca ctcgagaacg tgcctgtcca
     2401 cgcgcagtgg tgcatggcgg gcttgtttgc cgtgttcctg gtcgccggtc ttgtctttct
     2461 gctcgccctt cacatgatgg tcgttcccaa gctcattgcc ggtcgaaggg ccgagctccg
     2521 cgagtctgca tgacgccgag aatcgcccgc tacaaaaggt agaagtgccg acgccgcccc
     2581 tgtgcgtccg catagagccc gttgagccgc acgccagccc ggtaataccg atcgatcaag
     2641 tccttcagaa acggcagggc ccgcgcactg aagtaatcca actccggcgt ttcgtagctc
     2701 agcacaacca gcactcggtc gagcggactg agtaggctcg attcgaggcc caaaccgtgc
     2761 gcctcgaggg tttgactgag agaacagagg tgacgcgcct ctcggcgtgt ctgcccggcg
     2821 aggaaaccca gcgcaccttc tatgtgaatg cgaaagcgca gacggggcca ccagaccgcc
     2881 tttgctcaga gggcgggttt cgactcgttc gaccgacggt taggctccat tgtcgccgat
     2941 agcttcgacg ggacagcctt ccttggcgtc cttgcactgg ctctcttcgt cctcgttctc
     3001 aggctgtttg cagacgaacg agtagccgcc gtcctcgttg cgctggaaat tgttcggagc
     3061 ggtctcgcga cagagatcac agtcgatgca ttgattgtcg acgtagtact tgccggggac
     3121 gttttctggg tatttgtttg cgatgtctgc catacaatcg attgggttgt tagtcgttgg
     3181 tacttatgaa gacgtgatcg ttaaaatcaa gcctgattcg cgtgcgtccc ttcgggcggc
     3241 ggacgctcgc cccgatccgg gcgcgaaccg cggtcgtagc gttcacctcg attcggcctt
     3301 tccctgcgct ccggcctttc cgatcggtgc tcgctccgcg gcggtcgatc gccttccgga
     3361 cgccgagccg ggcgttctcc ctcccccgac ggctgctttt cgcgctcgag cttgtcgcgt
     3421 tcctccatgg ccgccttgcg ggagaggcgg acccggcctt tttcatccac cccgaggcat
     3481 ttgacccaga tttcgtcgcc gagcttgacg atgtcttcgg tctgtttgac ccggaagttg
     3541 gcgagttcgc tgatgtgcac aaggccgtcc tgcccgggga acacctcgac gaaacagccg
     3601 aaatccttga tggtgaccac cttgccccga tacagcttgc ccacctcgat ctcggcgctg
     3661 atcgcctcga cctctcgacg ggccacttcc atgccctcga cactggtcga gtagatctgc
     3721 accgtgccgt catcctcgat gttgatctcg catccggact catcgacgat gcgcttgatg
     3781 ttcttgccgc ccggaccgat gagcgcgccg atcttctccg gattgacctt gagcgtcagg
     3841 atgcgcgggg cgtacttgct gagttccttg cggggctcgg cgatcgtttc ggccatgatc
     3901 ttgagaatct tgagccgcgc aacacgcgcc ttctcgatcg cctcggccat cagcaggtgc
     3961 gggatgccct tgagcttcag atcgagctgg aaacccgtca cgcccttctc cgtcccagcg
     4021 atcttgcagt ccatgtcgca gaagaagtcc tcggccccga tgatgtccgt cagcatttca
     4081 tagcgggcga tcttcccgct cggaccgtat tcggtgcaga gcccgatgct gacacctgcg
     4141 acgggccgga ccagcggcac gcccgcatcc atgagggcga ggctgccgcc acacacggtc
     4201 gccatcgatg tgctgccgtt cgactccatg atctcgctgg ttacacgcac tgcgtaaggg
     4261 aactccttga gcggcaccat cggctcgatc gaccgttccg ccaacgcacc atggccgatt
     4321 tcgcgccggc ccggacccgc gatgcgtccg gtctcaccga cggagaaatt ggggaaatta
     4381 tagtggagaa tgaactgctt ttccttgtct ccgccggcat aggcatcgat ttcctgagaa
     4441 tcctcggtgg tgccgagtgt tacaagggcc agggcctggg tctcgccccg ggtgaacaag
     4501 gccgatccgt gggcgcgcgg caggagcccg acgctgcaac tgatcgggcg cacctcgtcc
     4561 atcgcacgtc catcgagccg ttttccgtgg ttgaggatct gatcccgtac ggcctccttt
     4621 tgcaggtggt tgaaggtatc tttcagaacg aactcggtga tcttctcagc gccgaacttc
     4681 tcgatcagct tgggcgccac ccgctcggtg acgactttcc aggcggcttc gcgctcgtgt
     4741 ttgccgggtg tcagcagcgc cggaacgctc tggtcgccgg ccagcgcctt ggcttcgcgc
     4801 agaatttcgt cgggaaccac gttgaccgtg atcgagcgtt tcggccttcc ggccttcgcc
     4861 gcaaggtctt tctgggcgga gatcagcggc tgacagcaaa gctgggcgaa gctgagggcg
     4921 ccgatgaaat ccgcttctgg aatctcgagg cccgatcctt cgaacatgac catctcggtc
     4981 tcgctcccca catagaccag atcgaggtcg ctctccgcct tctgcgcgtg ggtagggttg
     5041 acgacgaact ggcccttgat ccggcccacg cggacggcgg cgatgggccc ggcccagggg
     5101 atgtcgctga ccatcagggc ggccgacgcc ccgttgatgc tcagaatgtc ggggtcgttt
     5161 tcgccgtcgg cgctcagcag aatgctctgc acttgcacct cgttgaacca ccctttcggg
     5221 aacaacggcc ggatgggccg gtcgatcatc ctcgatgtca ggatctcctt ctcggtgggc
     5281 cggccttcgc gtttgaaata cccgccgggg aatttacccg cagccgccgc tttctcccga
     5341 taatcgacag tcaacgggaa aaactcctgc ccctcccgcg gcgtggcggc cgcaaccgcg
     5401 gccacgatca caatggtttc cccaagccgg acggtgactg caccgtcggc ttgcttggcg
     5461 agcagaccgg tttcgatgtg gatttggccg gccccgacgg ggacggtgac tgtttcaggc
     5521 atacgaactt tcattctgcc tgccgacccg gaggaacttc agtcgaccgc ccacgcggag
     5581 aagccgaatg aaatttccct aagccggcag gacttggtgt ttgttggcgc ggtgtgtcta
     5641 aggcgtcgct cccgccggcc cgcgctttac cggccgactc tccccaccgg ggtgagcgag
     5701 ctgttcgccc aaggatttta ctatggctgg agccgcgaca catgatcgcg gactgctcgc
     5761 ggaggccctt acctgcgcag cttgagcttc ttggtcaccg caagatagcg gtccgagtcg
     5821 gtcgtgtgca ggtagtccaa cagacgccgc ctttggccca ccatcatcag caagcctcga
     5881 cgcgagctgt ggtctttacg gtgcttctgc aagtggtcgg tgagctgatt gatccgctcc
     5941 gtcaaaagcg ctatttgcac gtcggcagat cctgtgtcct tctcgtgcaa ccggaaatcc
     6001 gtgatcgtct tcgtctttgc ttccataccc gttctgtcca gccaaaatca atgaagcgca
     6061 aaccataagc cacggtacgc ctgactgtaa agaactttat cggctttccc tcgtagccac
     6121 agggctgctc taaagtcgaa ctgggcgggt caataggcga atgacctgtc ccgg
//
LOCUS       DAOQRO010000205         6160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12818_length_6160_cov_4.025717, whole genome shotgun sequence.
ACCESSION   DAOQRO010000205 DAOQRO010000000
VERSION     DAOQRO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12818_length_6160_cov_4.025717"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKK20_08515"
     CDS             complement(<1..519)
                     /locus_tag="PKK20_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNU99970.1"
                     /translation="MKTLLFGPVLFAWLAAAADLPPLVVPRGVGVNIHFNRGHHADLD
                     LIAAAGFKFIRMDLGWYGIERERGRYDWSAYDELTANIEARGIRPLYILDYSNPLYEE
                     TVATRDPVSGRDHRATASPQRPESVAAFARWAGEAARHFKGRRVVWEIWNEPNISFWK
                     PKPDVGQYTTLAL"
     gene            740..3376
                     /locus_tag="PKK20_08520"
     CDS             740..3376
                     /locus_tag="PKK20_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNU99971.1"
                     /translation="MIDLARSLAVLVGLETVLAWAAMPAPEPGLDADAPAANSPACLD
                     RAAAPQQQSQQQRQRRERGTRAAGRKPGVYRAKVVPHWFADNTRFWYRNDLADSREFI
                     VVDARLGTRAPAFDHESVARQIGEDGANELLSIEELRFSPDGLTVQLLGASKTWSLDL
                     ATDRVQPAPAREDGPPSEGLPADDRERPSARTGPETEIIFDNRLDREVRLFWLNDEGV
                     RQSYGAVPPRSRRVQHTYGGHVWLVTNEQGDTIAVFEAADRPETALITGARPQPPSSP
                     PARSRERRRSTDRSPDGRWTAFVKDANVYLRDEEGEEIRLSEDGREDNVYRGLEWSPD
                     STTLVAWRVQPGDRKEVYLIRSSPRNGGRAQFESRPYALPGDRFARHELNIFDVATRR
                     QIKPEVDTFEHEWESPRLRWRLGGQRFAWQQVDRGHQRLRLIEAECVTGNTRTIIDER
                     TETFIWTAHTENLDLAHVNWLEQSDEIVYASERDGWRHLYLVDVNEGRIRNPITQGDW
                     VVRGIDRIDEQARQVWFRASGRNPDQDPYFIHHYRVNLDGTGLVALTEGNGTHTIEFS
                     PDRACYIDCWSRVDQPPVSELRRSFDGALVCLLEEADISELKSSGWEAPEVFVAKGRD
                     GQTDIWGIVCRPRNLDPAKKYPVIEHIYAGPQSSYVPKSFSPEPRFRALTDAGFIVVQ
                     VDGMGTANRSKAFHDVCWRNLKDAGFPDRILWHKAIAAKYPYYDVNRVGLYGTSAGGQ
                     NAAGGVLFHPDFYKAAVANCGCHDNRMDKASWNEQWMGYPVGPQYAESSNIEHAARLQ
                     GGLFLVVGEMDTNVPPESTLRFVDALVRANKDFELLIVPNGGHGSGGEYYQRRMRDFF
                     LRNLQGVQPPNWNAEASPPGQG"
     gene            3633..3845
                     /locus_tag="PKK20_08525"
     CDS             3633..3845
                     /locus_tag="PKK20_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99972.1"
                     /translation="MALRNILCNPAVIAPIPGMISIRQVDNAANVVKERLQLDAAERA
                     ELDQAAKRMWARLPADYQWLRDWEYV"
     gene            4003..4311
                     /locus_tag="PKK20_08530"
     CDS             4003..4311
                     /locus_tag="PKK20_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99973.1"
                     /translation="MLQTRRAIVEEIEAIASQPRLIVQTSPPEGTVVPAGPRAVNLRG
                     LGPPGAKVTVNGQTVANTRPSGYFSHAVFLGDDQPTMIVAVDHNGAQRAVSRTFPLAD
                     "
     gene            4633..>6160
                     /locus_tag="PKK20_08535"
     CDS             4633..>6160
                     /locus_tag="PKK20_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HNU99974.1"
                     /translation="MVYTPSYDEEIKGIFELAKQQRWEEAETQAAALWARDPQDSAAI
                     RLRDWVRQRRERSREQALEDQIRSIEANNSVFNPTLKDLATEKKDRGLPPRKDVRDAI
                     QQIKATPYIPETYGRTNYLRGLMFDFESRQGRMAKILEKEVSIVLEDATLESIIFDVG
                     QNEGINFVADKSLPAFQTKLSVNMKQVKLSEFLRYISRNLNLQFQIGEDLIWVVDSKD
                     PTKVLEETRFYRLQRGFVLPAEFGPPEVQTTTTTANNVRTVTEVQKMSKFVNDQAATM
                     PSIEQAITNFFTGTYLIDFERNVIVARGTPEQLNILESVIENFDQPIQQVLIEARFVT
                     VTQAAFLRLGMIWGTGLATTTVEPTSDQTGMAFLGVAPGFQWNWTNVFGVSELTATLA
                     ALEQSGESQTLSAPRLTLINNRPGIINDGKVQYYYEQYQVKATILERRSTSQLVPFEK
                     PTKITSGASLQVLASIGGDGNTILRALSPRVNADVKMVPFMTITDTDDAGNIVSKQDI
                     N"
BASE COUNT         1279 a         2002 c         1862 g         1017 t
ORIGIN      
        1 cagcgcaagc gttgtgtact gacccacgtc aggtttcggt ttccagaacg agatgttcgg
       61 ttcattccag atttcccaca ccacgcgccg gcccttgaaa tgccgggccg cctcacccgc
      121 ccaccgggca aaggccgcca cactctcggg ccgctgcggc gaggccgtcg cccggtggtc
      181 ccggccggaa accggatcgc gcgtcgccac ggtctcttcg tacagtggat tcgagtaatc
      241 caggatgtag agcgggcgaa taccccgcgc ctcgatgttt gcagtgagtt cgtcgtaggc
      301 cgaccagtcg taacgccccc gttcgcgctc gatgccgtac caccccaaat ccatgcggat
      361 gaacttgaac ccggcggcgg cgatcagatc gagatcggca tgatgcccgc gattgaagtg
      421 gatgttcacc ccgacgcctc gcggcacgac caggggcggg agatcggcag cggccgccag
      481 ccacgcgaac agaaccggac cgaagaggag agtcttcatg gaacgttcca tcatggagtt
      541 tgcaggtaca gcaattcaga ggccgggtca acacggttcc tcgaattgga ttctcgaatc
      601 aaagtggaca gaagaacgga cctcgatcag cacgaacgcc gaacccaacg cgatcctgga
      661 cgtcaagtca tatggcacct gcggacgttg accgctgccg cggtctccgc cacacttccg
      721 actccaacta cgacgcaata tgattgatct ggcacgatct ctggccgtcc tcgtgggcct
      781 ggaaaccgtc ctggcatggg ccgccatgcc tgcacccgag cccggcctcg atgccgatgc
      841 gcccgcggcg aattcgcccg cctgcctgga ccgagccgcc gctccgcaac agcaatccca
      901 gcaacagcgg cagcgacggg agcgcggaac acgcgccgcc gggcggaagc ccggcgtgta
      961 tcgggcaaag gtcgtgcccc actggttcgc ggacaacaca cgcttctggt accggaacga
     1021 ccttgccgac agccgcgagt ttattgtggt ggatgcgagg ctcggcacgc gggcgcctgc
     1081 gttcgatcat gagtcggtcg cgcgccaaat cggcgaggat ggcgcgaacg aacttctgtc
     1141 gatcgaggaa ctccggttct cgcccgacgg cctgaccgtg cagttactgg gcgcctcgaa
     1201 aacctggtcc ctcgacctcg cgaccgacag ggtgcagcct gcacctgctc gggaggacgg
     1261 accgccctct gaaggcttgc cggccgacga tcgcgagcgg ccctcggccc ggactgggcc
     1321 ggagaccgaa atcatctttg acaaccgcct cgatcgcgag gtgcggcttt tctggctaaa
     1381 tgacgagggc gtccgtcaaa gctatggcgc tgtcccgccc cgcagccgca gggtgcaaca
     1441 cacctacggc ggacacgtct ggcttgtgac gaacgagcag ggtgacacga tcgcggtctt
     1501 cgaagcggca gaccgtccgg agaccgcgct catcaccggc gcgcgccccc aaccgccctc
     1561 gtcgccgccc gcgcgctcga gggaacgacg caggagcacg gaccgctcgc ccgacgggcg
     1621 atggaccgcc ttcgtcaagg acgccaacgt gtacttacgc gacgaggaag gcgaggaaat
     1681 ccgcctgagc gaggatggcc gggaagacaa cgtataccgc ggactcgaat ggtcgccgga
     1741 ttccacgacg ctggtggcct ggcgcgtcca gccgggcgat cggaaggagg tctatctcat
     1801 tcgatcctcc ccgcgcaacg gcggccgagc gcaattcgaa tcccgtccct acgcattgcc
     1861 tggcgaccgg ttcgccaggc acgaactcaa catcttcgat gttgccacgc gccgccagat
     1921 caagcccgaa gtcgacactt tcgagcacga atgggaatcg ccgcgtctga ggtggcgtct
     1981 gggcggtcag cgttttgcgt ggcagcaggt ggaccgcgga caccagcgtc tgaggctcat
     2041 cgaggccgag tgcgtcaccg gcaacacgcg gaccatcatc gacgagcgca cggaaacatt
     2101 catctggacg gctcacacgg agaacctgga cctcgcccat gtcaactggc tggagcagtc
     2161 cgatgagatc gtctatgcgt cggaacgcga cggctggcgg catctctacc tggtcgatgt
     2221 caacgaaggc cggattcgca accccatcac ccagggcgat tgggttgttc gcggcatcga
     2281 tcgcatcgac gaacaggcgc ggcaggtctg gtttcgggcc agcgggcgca accccgacca
     2341 ggacccctac ttcatccacc attaccgggt gaacctcgac gggaccggcc tggtggcgtt
     2401 gacggaaggc aacggcacgc acacgatcga gttctcgccc gaccgcgcgt gttacatcga
     2461 ctgctggagc cgtgtggacc aaccgccggt gagcgaactg cggcgctctt tcgatggcgc
     2521 gctggtctgc ctgcttgaag aggccgacat ctcggaactc aagagcagcg ggtgggaggc
     2581 gcccgaggtc ttcgtagcca aaggccgcga cggccagacc gacatctggg ggattgtctg
     2641 ccgcccgcgg aatctcgatc cggccaagaa gtaccccgtc atcgagcata tctacgctgg
     2701 accgcagagt tcctacgtgc caaagagctt ctcgcccgag cctcggtttc gcgcgttgac
     2761 cgatgcgggc ttcatcgtcg tccaggtgga cggcatggga acagccaatc gttccaaggc
     2821 gtttcatgac gtctgttggc gaaatctcaa ggacgccgga ttcccggatc gcatcctctg
     2881 gcacaaggcc atcgccgcga agtaccccta ctacgatgtg aatcgcgtcg ggctttacgg
     2941 cacatccgcc ggcggacaaa acgctgcggg aggagttctg tttcatcctg atttctacaa
     3001 ggccgcggtg gcgaactgcg gatgccacga caaccgcatg gacaaggcgt cgtggaacga
     3061 acagtggatg ggctatcccg tcgggcccca gtacgccgag tcgtcgaaca tcgagcatgc
     3121 ggcccggctt cagggcggcc tgttcctggt cgtgggcgag atggacacca acgtgccgcc
     3181 cgagtccaca ctgcgcttcg tcgatgcgct cgtccgggcg aacaaagact ttgaactgct
     3241 catcgtcccc aacggcggcc atggctcggg cggcgagtac tatcagcggc gcatgcgcga
     3301 tttcttcctc cgaaatcttc agggcgtaca gccgccaaac tggaatgcgg aagcatcgcc
     3361 gcccgggcaa ggctgaacgg caaggctgaa cgggcttgct ttggcggctg cggatgttca
     3421 tgactccccc gccaccatga acaacaaccg ccatcccgtg aaagaggatt tcactgccaa
     3481 gtccaaggtc cggccgaccg acagcgtgtt cgaggccatc cggcagcacg acgtcggcat
     3541 gttcggcatc aagcccttcg ccagcaattc tcttcaaagg cagcagctcg cccgaggacc
     3601 ccaacgcgcc cgaggacgat cgtcgggcac gcttggcact gcgcaacatc ctctgcaacc
     3661 cggccgtcat cgcgcccatc ccgggcatga tcagcatccg gcaagtggac aacgcggcca
     3721 acgtcgtcaa ggaacgccta caactcgacg cagccgagag agccgaactc gaccaggccg
     3781 cgaagcgaat gtgggctcgg ctcccggctg attaccagtg gctcagagat tgggaatacg
     3841 tttagcgagg ctccgcctca gaccgacgag cggtgtcacc cattcgtgct cgctgggttt
     3901 cgacgacggt ctgtggagtt cgacgcctcg ctaagcggcg ccgctgcacg gcgcaaaaac
     3961 ttgactcgat gacaacgaat ttcgtcgagg gagtgagacg tcatgctcca aacacgccgc
     4021 gccatcgtcg aggaaatcga ggccatcgct tcccaacctc ggttgatcgt ccaaacctcg
     4081 ccccccgagg gaacggtcgt gccggccggg ccaagggcag tcaacctgcg cggattgggc
     4141 ccgcctggcg cgaaagtgac ggtcaacggc caaacggtcg caaacacgcg ccccagcggc
     4201 tacttctcgc acgccgtctt tctgggcgat gaccaaccga caatgattgt agccgtcgac
     4261 cacaacggcg cgcaacgtgc tgtgtccagg acattcccgc tcgccgattg aggcgggccg
     4321 cacgcggcgc aaggatagtt gggcaaggat ttgcgtccga cagaaaaggc cgatttagtc
     4381 ttgcgggcaa gcgcctgata ggcaaggaat agcactggtc agccctgaat ggccaacccg
     4441 gggctggcgt cgatgaatct tactatggcg aaacctgacg gatcttggcg catccgagtc
     4501 ggctgcggag cgctggcact cttgcttgtc acctcgtgtt accggtccac tcaatcgtct
     4561 ggcgcgagca aacctgccaa agccacgccc actgcctcga agagcagggg ccgcgccacg
     4621 agggcgccca aggtggttta cacaccctcg tacgacgagg aaatcaaagg catcttcgag
     4681 ttggccaagc agcagcgttg ggaggaggcc gaaacgcagg ctgcggccct ctgggcgcgc
     4741 gatccgcagg attcagccgc gattcgtttg cgcgattggg tgcggcaacg gcgggagcgc
     4801 agtcgggaac aggcgctcga agaccaaatc cgctcgatcg aggccaacaa ctcagtgttc
     4861 aatccaaccc tcaaggatct cgccacggag aagaaggacc gcgggttgcc tccacgcaag
     4921 gacgtgcggg acgccatcca gcagatcaag gcgacgccct acatccccga gacctacgga
     4981 cgcacgaatt acctgcgggg cttgatgttc gatttcgagt cgcgccaggg ccgcatggcc
     5041 aagattctcg agaaggaagt atcgatcgtc ctcgaagacg ccacgctcga gtcgatcatc
     5101 ttcgacgtcg gccagaacga agggattaat tttgtcgcgg acaagtcgct tcccgccttc
     5161 cagaccaagc tcagtgtgaa catgaagcag gtgaaactct ccgagtttct ccgctacatt
     5221 tcgcgcaacc tcaatctgca gttccagatc ggcgaggatc tcatctgggt cgtggacagc
     5281 aaggacccga cgaaagtgct cgaggagacg cgattctacc ggctccagcg gggtttcgtt
     5341 ctgcccgccg agttcgggcc gccggaggtg caaaccacca caaccaccgc caacaacgtc
     5401 cgaaccgtca cggaagttca aaagatgagc aagttcgtca acgaccaggc ggcgaccatg
     5461 ccatcgatcg aacaggccat caccaatttc ttcaccggga catacctcat cgatttcgaa
     5521 cgcaatgtca tcgtcgcccg cggcacgccc gaacagctca atatcctcga gagcgtgatc
     5581 gagaacttcg accagccgat ccagcaggtg ctgatcgaag cccggttcgt cacggtcacc
     5641 caggccgcct tcctgcgcct gggaatgatc tggggcacgg gcctggcaac caccaccgtc
     5701 gagcccacct ccgatcagac cggcatggcc tttctgggcg tcgctccagg attccagtgg
     5761 aactggacca atgtgttcgg agtctcggaa ctgacggcca ccctggcggc gctcgaacag
     5821 agcggcgaga gccagacatt gagcgcgcca cgcctcaccc tgatcaacaa ccgcccgggc
     5881 atcatcaacg acggcaaggt ccagtactac tacgaacaat accaggtcaa agcgaccatc
     5941 ctcgaacggc gctccacatc gcaactggtc cccttcgaaa agccgaccaa gatcacctcg
     6001 ggggcctcgc tgcaagtgct ggcaagcatc ggcggcgacg gcaataccat cctgcgcgcg
     6061 ctcagtccaa gggtgaacgc ggatgtcaaa atggtgccgt tcatgaccat caccgacacc
     6121 gacgatgcgg gcaatatcgt gagcaaacag gacatcaacc
//
LOCUS       DAOQRO010000206         6144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12860_length_6144_cov_5.214157, whole genome shotgun sequence.
ACCESSION   DAOQRO010000206 DAOQRO010000000
VERSION     DAOQRO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12860_length_6144_cov_5.214157"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2332)
                     /locus_tag="PKK20_08540"
     CDS             complement(<1..2332)
                     /locus_tag="PKK20_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNU99975.1"
                     /translation="MALGSWILTSFGSASPVLESGPRGRREVIDVWDTDVGLPRNSVT
                     AIVRTRDGYLWFGTPNGLVRFDGLRFRVFDALSTPGLSDSHVVSLLEDRAGNLWIGTE
                     TGGVALFGDGVFTSIGIGQGSTARRLVASVQDATGAVWLYTADGQLWRHANSQFSPFV
                     FGFDRPTARRSIIVDTAGRVWAGTDGRVASIRPATGAGHLESQIDEEVAVGRLDALVA
                     RAAGGYWRLGDGRVQQFEGTRCTVDLGAYPWGAVPVACACEDRQGDLLVGTLGAGVYR
                     FEGGGRVRRHSTSDGLSNNYIFSLCVDSDGSLWVGTDGGGLNRIRPQAFEAIDVSPDL
                     GVGVVQTVCTDAKGGLWVGSNGGGLAYYLGSERRRYGPEQGLTHLSVWSVLVDRAGDV
                     WVGTQGGGLFKLREGRLLRVGGDAIPRLVAALHEDRQGRLWVGTTGGLARRDPDGWRL
                     FTTRDGLSNDDVRAIVDDGAEGLWIGTRGGGLNRWSQGRFTAWHRQDGAPGEDVSSLW
                     LDRDGVLWVGTLGNGLGRFENGAWTLYTTREGLASNVIGYQLEDDENCLWLGSNTGLM
                     RVARRDLNAFARGGTGLVDVRTYGKPDGMPTRECTMGSQPGAWRADDGTLWFSTVRGL
                     TGIHLGRLRVNPRPPPVVIEAVLIDGQPQGTSSIESRWPERVVVPAGREHLVIQYTSI
                     NLVAPDKGRFRYRMEGHESAWTEAGNIRVARYSRLPPGEYRFCVTASNEDGIWNEVGA
                     SFAVSVETPLWQTPWFLGASASLLLGAIVGLVYLIST"
     gene            2700..5837
                     /locus_tag="PKK20_08545"
     CDS             2700..5837
                     /locus_tag="PKK20_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNU99976.1"
                     /translation="MRCLGFILGPLALGACFAAAADVNRLSVEPPAGAAWPRFSISTL
                     SNRVVRLEASGDLETWQEIARSHDVLISYPDMSATEEDTRFYRASIVPRTAVDDWKNQ
                     ARFPDDSLLSPAPAYGEILPRWIKFVIVLADPHRVHFQDSSKYSFHYDFARARLAPFQ
                     SFTREQFDAVSLWRANQQVVLGTVLFGPTTNLREIGIQFVGNDPYPPEQVADWFDRVR
                     ASLVAGPEVKVFYFPTYEQQGAAELNRPYFDSRGIAISSGARWVIADQCYAAGWALGR
                     LVHVPAAELDAYYRDGRLRPDDILVVETVPSKIPPVAGIVSIVPTTPNSHVALLAQSF
                     GIPCVYFADAASRDWVLGLLGQEVVLLARSNYGACDLRIASVEGQLSDDLRLELLALK
                     TPPALELTPKITSGTIGISAEGLRPADIARVGGKAANFGLLRRAIPAYAPTPALALTF
                     DLWDAYLDQTLPTGTTTLRAAIDAKLSGYSWPPEMAAVQDALAEVRTLFTDIADFDPA
                     QRQAILTLLDAAGFAPDRKIRFRSSTNVEDSEQFSGAGLYDSYSGCLADELDGDDAGP
                     SQCDPSDSRERGVFRALRKVYASFFNQNAFLERLRHRVDEADVGMAVLVHHSTPDEFE
                     LANGVATAEIWKGDSPNIRSFSARLVTQLGALSVANPEANAWPEEVKADIWSDGAPYL
                     STLRSSSLVPLGDHVLAWETEYRDLAIQLDKAARAYEAEFPERRTFLLDFEYKKIAPE
                     GRLLVKQIRELPQPSNTTTLTPWLLNQTNRYVLAQGEHGNAFAFHRLKSRWTLTTGNL
                     RLTDANLADTIYRTVNAELLDGMAVSGFGGLLSDLPEFAHCTDGEYTIDSWALGTGKD
                     RRVFELQTWLPRQVSVNGGPIVLLSDGTIFLKARYAQPQPVINYDGPDATSSDEATLT
                     PARTVSPLSLLQTRKLAGKGITVDTRFYWPPNPTGPTAGYTAPLEAWVETRIEGLASQ
                     VIVLRHDLAQTYHPGHHNFFEEFIFDPHLEPGIDPTILSELAARNIRALYGTWSFDTS
                     TTFWIWGLDNTLRRL"
BASE COUNT         1131 a         2228 c         1725 g         1060 t
ORIGIN      
        1 gggtcgagat caaataaacc agcccgacaa tcgccccgag aaggagcgac gccgaggccc
       61 cgagaaacca tggcgtttgc cagagcggcg tttcgacact cacggcgaaa ctcgcgccga
      121 cctcattcca gatgccatcc tcgttcgagg ccgtgacgca gaaccggtat tcacccggcg
      181 gcagccggct gtagcgcgcg acacggatgt tgcccgcctc ggtccaggcg ctctcgtgcc
      241 cttccatccg ataccgaaac cggcccttgt ccggcgcaac gaggttgatg ctggtgtatt
      301 ggatcacgag atgctcgcgc ccggcgggca ccaccacccg ctccggccat ctcgactcga
      361 tcgagcttgt cccctgcggc tgcccgtcga tgagcaccgc ctcgatcacc acgggcggcg
      421 gccgcgggtt cacgcgcaac cgtccgaggt ggatgcccgt cagcccgcgg accgttgaaa
      481 accaaagcgt gccgtcgtcc gcccgccatg ccccgggttg tgatcccatc gtgcactcgc
      541 gagtcggcat gccgtccggc ttcccgtagg tgcgcacgtc caccaacccc gtcccgccgc
      601 gtgcgaatgc gttcagatcc cgtcgcgcca ctcgcatcag accggtattt gatccgagcc
      661 agaggcagtt ctcgtcgtct tcgagctggt atccgatgac gttgcttgca aggccctccc
      721 gagttgtgta cagcgtccag gcgccgttct cgaaacgtcc aagaccgttt ccaagcgttc
      781 ccacccacag aacgccatcc cggtcgagcc acaacgacga gacatcctct cccggcgcgc
      841 cgtcctgtct gtgccaggcc gtgaaccgcc cttgactcca acggttgagt ccgcctcccc
      901 gggttccaat ccataagccc tccgcgccgt cgtcgacgat ggctctgaca tcgtcgttcg
      961 agagaccatc gcgcgtcgtg aagagccgcc agccgtccgg atcgcgccgc gccaatccgc
     1021 ctgttgtgcc gacccaaaga cgtccctggc gatcctcgtg cagtgcggcc accaggcgcg
     1081 gaatcgcatc gcctccgacc cgcagcagcc gcccctcgcg caacttgaac aacccgcctc
     1141 cttgcgttcc cacccagaca tccccagccc gatccacaag caccgaccag acggacaggt
     1201 gggtcaaccc ctgctctgga ccgtagcggc gtctttccga acccaggtaa taggccagcc
     1261 ccccgccgtt ggaaccgacc caaagcccgc ccttggcatc tgtgcagacg gtctggacca
     1321 cccctacccc caagtcgggc gagacatcga tcgcctcgaa cgcctggggt ctgatccggt
     1381 tgagcccgcc cccgtcggtc cccacccaca ggctgccatc ggagtcgacg cacagtgaaa
     1441 agatgtagtt gttcgagagc ccgtcgctcg tcgagtgccg ccgcacacgc ccgccgcctt
     1501 caaagcggta gacccccgcc ccgagcgtgc ccaccagcag gtcgccctgc cgatcctcgc
     1561 acgcacacgc gaccggcacg gcgccccacg gatacgcccc caaatcgacc gtgcatcgag
     1621 tgccctcgaa ctgttgcacg cggccgtccc ccaaccgcca gtagccgccc gccgcgcgcg
     1681 ccacaagcgc gtccaaacgt cccaccgcca cctcttcgtc gatctgggat tcgaggtgcc
     1741 ccgcgcccgt tgcggggcgg atcgaggcca cacggccatc cgtcccggcc caaacccgtc
     1801 ccgcggtatc gacaatgatc gatcgccggg ccgtgggccg atcgaaaccg aacacgaacg
     1861 ggctgaattg actgttcgcg tggcgccaga gctgaccgtc cgcggtgtac aaccagacgg
     1921 cacccgtcgc gtcctggacg gacgccacca aacgccgggc cgtcgatccc tgaccgatgc
     1981 cgatgctggt gaagacgcca tccccgaaca aggccacacc tccggtctcc gtgccaatcc
     2041 acaggttgcc cgcccggtct tcaaggaggc tcacgacgtg gctgtcgctc aacccgggcg
     2101 tgctgagcgc atcgaacacc cgaaaacgaa gtccgtcgaa acgcaccagt ccattcgggg
     2161 tgccgaacca cagatagccg tcccgagtcc gaacgatcgc agtcaccgaa ttgcgcggca
     2221 gcccgacatc cgtgtcccag acatcgatca cctcccgcct cccgcgcgga cccgactcga
     2281 gcacaggcga cgccgaaccg aacgatgtca gaatccatga gccaagcgcc acccacgatg
     2341 tcagggtcga cagacggcgc gcggcactcg cggagccgaa cgtgccagtg ccgcgccgtt
     2401 gactgtatct gtttctcaga ccatgcgagg gcacacgcga tttacgtacg gccagccgac
     2461 aaggtaaaga agtttgaaag gcgcggccgc aaatcccttg aggccggccc ccttccatcc
     2521 gtgtttcatc catgttcgat ccgtggctgc cccctccctg atcggtattt catccgtgtt
     2581 cgatccgtgg ctctcgattg atctcaacaa ccccgcccac gatcgcgatc cacactccac
     2641 accgagttca tcctggaaac cgctcgctcc tcgacgggtt tcgctctatg gtccgcacaa
     2701 tgcgttgcct tggattcatt ctgggtccgc tggccctcgg ggcctgtttc gcggcggctg
     2761 ccgatgtcaa tcgcctgagc gtcgagcccc cggcgggcgc ggcgtggccc cgcttctcga
     2821 tcagcacatt gtcgaaccgt gtcgtccgac tcgaagcgtc aggagacctc gaaacctggc
     2881 aggaaatcgc gcgttcccac gatgtcctga tctcctaccc ggacatgagc gccacggagg
     2941 aagacacacg attctaccgc gcgtcgatcg ttccccggac cgcagtcgac gattggaaga
     3001 accaggctcg ttttcccgac gactcccttc tctccccagc gcccgcgtac ggagagattc
     3061 tcccccgctg gatcaagttc gtcatcgtcc tggccgatcc gcatcgagtg cacttccagg
     3121 acagctcgaa gtactccttc cactacgatt tcgcccgcgc ccggctggcg ccgttccaat
     3181 cgttcacacg cgagcaattc gatgccgtct cgctctggcg cgccaatcag caggttgtcc
     3241 tgggcaccgt tctctttggc ccgaccacga atctgcgcga aatcggcatt cagttcgtcg
     3301 ggaacgaccc ctatccgcca gagcaggtgg ccgattggtt cgaccgggtg cgcgcgagtc
     3361 tcgtcgcggg gcccgaggtc aaggtcttct atttcccaac ctacgaacaa cagggcgcgg
     3421 ctgaactcaa tcggccctat ttcgacagca gagggattgc gatcagctca ggagcgcgct
     3481 gggtaatcgc cgatcaatgc tacgccgccg gctgggccct gggccgactc gtccatgtgc
     3541 cggcagcgga actcgacgcc tactaccgcg acgggcggct gcgcccggat gacatcctgg
     3601 ttgtggagac tgtgccgtcc aagatcccgc cggtggcggg aattgtgtcg atagtcccga
     3661 caactcccaa ttcgcatgtc gctctcctgg cgcagtcgtt cggcatcccc tgtgtgtact
     3721 tcgcggacgc ggcctcccgc gattgggtgc tgggcctgct cgggcaggaa gtggtgttgc
     3781 tggcccgcag caactacggc gcctgcgacc tgcgaatcgc aagtgtcgag ggccagcttt
     3841 ccgatgacct gcgccttgaa ttgctggcgc tcaagacgcc gcccgcactc gaactcaccc
     3901 ccaagataac ctcgggaacc atcggcatca gcgccgaagg actgcgcccg gccgacatcg
     3961 cacgcgtggg aggcaaggcc gccaatttcg gcctgctgcg ccgcgccatc cccgcatatg
     4021 cgcccacgcc cgcgctggcc ctgacgttcg acctgtggga tgcttacctc gaccagaccc
     4081 tgccaacggg caccaccacc ctgcgcgccg cgatcgacgc gaaactgagt ggatactcct
     4141 ggccgcccga aatggcggcg gtccaggacg cgctggccga ggtccgcacg ttgttcacgg
     4201 acatcgccga cttcgatccc gcgcagcgcc aggccatcct gacgctcctc gacgccgctg
     4261 gatttgcgcc ggaccggaaa atccgcttcc ggagttccac caacgtcgag gacagcgagc
     4321 agttcagcgg agcgggccta tacgacagtt acagcggctg cctggccgat gaactcgacg
     4381 gcgacgacgc cggcccgagc cagtgcgatc cgtccgattc gcgggagcgc ggcgtgtttc
     4441 gcgccttgcg caaagtgtac gcgagtttct tcaaccagaa cgcctttctc gaacgcctgc
     4501 gacaccgcgt ggacgaggcc gatgtcggca tggccgtgct ggtccatcac tcgaccccgg
     4561 acgaattcga gctggccaac ggcgttgcca cggcagaaat ctggaaaggc gattcgccga
     4621 acatccgcag cttcagcgcc agacttgtca cgcagttggg cgccctctcc gtcgccaacc
     4681 ccgaagccaa cgcctggccg gaggaagtca aagccgacat ctggagcgac ggcgccccct
     4741 acctctcgac gctgcggtcg tccagcctcg tgccattggg cgaccatgtc ttggcatggg
     4801 aaacggagta ccgcgacctg gcgattcagc tcgacaaggc cgctcgagcc tacgaagccg
     4861 agttccctga gcgccgcaca ttcctcctcg atttcgaata caaaaaaatc gcgcccgagg
     4921 gccgactgct ggtcaagcaa atccgcgaac tcccgcagcc ctccaacaca acgacactca
     4981 cgccatggct gctgaaccag accaatcgct atgtcctcgc ccagggtgag cacggcaacg
     5041 ccttcgcctt ccaccgtctc aagtcgcgct ggaccctgac aaccggcaac ctccggctca
     5101 ccgacgccaa cctggcggac accatctatc ggaccgtcaa tgctgaactc ctcgacggaa
     5161 tggccgtgtc cggttttggc ggtctgcttt cggacctgcc ggagtttgct cactgcacgg
     5221 atggcgaata cacgattgac tcctgggcac tcggcacggg caaggacagg cgcgttttcg
     5281 aattgcagac ctggctgccg cgccaagtgt cagtcaacgg ggggccgatc gtccttctca
     5341 gtgacgggac tattttcctg aaggcccgtt atgcccagcc gcagccggtc atcaattacg
     5401 acgggccgga cgccacttcg agtgacgagg cgaccctcac gccggcacgc accgtcagcc
     5461 cgctgagcct gctacaaaca cgcaaacttg ccggaaaagg cattacagtc gacacccgat
     5521 tctactggcc tccgaatccc acaggcccaa ccgcgggcta caccgccccg cttgaagcct
     5581 gggtcgaaac acgcatcgag ggactggcat cccaggtaat tgtgctgcgc cacgatcttg
     5641 cccagaccta ccatcccggc caccacaact tcttcgagga gttcatcttc gatcctcacc
     5701 tcgaacccgg catcgatccg acgatcctgt ccgaactggc cgcacgcaac atccgcgccc
     5761 tctacggcac ctggtccttc gacacgtcca cgacgttctg gatttggggc ctcgacaaca
     5821 ccctgcggag gctttagcca acgcaccaac agttggatgc gcacggagac cgtgaatcgg
     5881 cttgtagcta tcccggcttg acgctgcttg aacgccagtg cctgcgcctt gtgttgtgcc
     5941 aagtcgaacg cccgggcggc accgggccat ctgcgtcgtt cctcggtcgg tcgagtatct
     6001 catgggatac actccctccc tcgggctttg catctggccc ggtggcgctc ccgccaaaat
     6061 aagaagtact ttgcacagac ccttgggatg ctttacgaga cccgcccgca tcggattcaa
     6121 gtccacgtac gcggccacgg cctc
//
LOCUS       DAOQRO010000207         6141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12867_length_6141_cov_5.673677, whole genome shotgun sequence.
ACCESSION   DAOQRO010000207 DAOQRO010000000
VERSION     DAOQRO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12867_length_6141_cov_5.673677"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKK20_08550"
     CDS             complement(<1..216)
                     /locus_tag="PKK20_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99977.1"
                     /translation="MVPTGPIEMSLYIVRHYGQPSIFIERLTLRPDGFASAHASYKGG
                     ALITRPLRFSGRELEVNLSTSAAGFLRV"
     gene            complement(654..2891)
                     /locus_tag="PKK20_08555"
     CDS             complement(654..2891)
                     /locus_tag="PKK20_08555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNU99978.1"
                     /translation="METRTASPSTDSLRTLPGDDLRQIMWRFADRYDLQMLVQSSRQV
                     ARGPVARLVANGGRNAHDWTPEKAALLPAFDESGITAAFLDPDHGGFIQGPKNLALAL
                     IAFELSWVDAGAATCSLAGNLGLAPIHERGTPEQRARYLAGAMPVQPGEDRRQVRAAF
                     ALTEPLPFVGVETGMLAGRVRVADWPAGQEPVLQVEKRGRFITNMGFANVVTAAVESG
                     DPRIKGSCLVILEEGDPGTWDRGTPTKKLVHQLSSTTDPVFSLRVPANRIVGGYTVKD
                     GVVIPNYSHSEVIEAVFRRTRVTVGLMTAAKLLSAIEPIMAYQRRRFRGGEGAPGTPR
                     YELGLQQKQDTVQRLVDIWAAGEASASLGFAAARIFDELDPLEKQRDAYLAEKKLTGR
                     AAFKALAATHTDALEYLALCGQPDDRRDSARFAALRDQPLVRFVLLDALANVLCPACK
                     LWNTGHGATMMREAVSLMGGYGVTEDCPGFLGQKWMDAQLEATYEGPEAVQRLQLSIT
                     MTNPLFLAQFRQWIVEMRQIASDRPGTGACTLATAMSLWLWTLEHVQKATDADGDKLY
                     HKTRQGVTFPLADALCWLLAARQFILDVIELDTQGAANPALAEGLPGFVRFFTDLCHV
                     QSARAAGETGRIAAEIVFGYNRHPAWTAEACHACYGEDELASLEAIIPGIQSSARACS
                     DVTEPGDAHPRKAGPCAAGAGLEPFLRLRTKLDGCLTGCRLAKDRAAEALTQTMIPEA
                     LDYPI"
     gene            complement(3335..3805)
                     /locus_tag="PKK20_08560"
     CDS             complement(3335..3805)
                     /locus_tag="PKK20_08560"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNU99979.1"
                     /translation="MKHFKAIAAMALNRVIGRDHRIPWHLPDDFRWFKRLTTGHVLVM
                     GRRTFESIGRPLPGRETIVLSRSRFQFPGVRTVSDLASLDPASDPRDFFICGGAEIYA
                     QTLPRCSDLYLTLVLRDIDGDTFFPPFEDRFDLAKTLETNSDFEIRHYVNRALV"
     gene            complement(3910..4677)
                     /locus_tag="PKK20_08565"
     CDS             complement(3910..4677)
                     /locus_tag="PKK20_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015465336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HNU99980.1"
                     /translation="MKRQHFLRLIRTVGCCMLLSWIGAHAATITLTGTVRDFNDTHPD
                     FEGSIGGHQPGQVESALGIDKKPIWRSPAKSGFTTADNFNQWYNNVAGVNLAAPLSLT
                     LDNTITPDPNVYTFTDSDFFPIDNELFGNQGRNHNFHFTYELHSSFTYAGGEMFQFTG
                     DDDLWVFINKSLVIDLGGVHGAISGSVNLDTLGLTPGGTYDFDLFFAERHTTQSNFRI
                     DTSIILVPEPAGCALLTAAALCGFASWRRPSPRSKRH"
     gene            complement(4779..>6141)
                     /locus_tag="PKK20_08570"
     CDS             complement(4779..>6141)
                     /locus_tag="PKK20_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNU99981.1"
                     /translation="TYAFRQKGTGIAPPNGPALIKPGTETLPAILKRAGYTTAVIGKW
                     HLGLGAPAPDWNGELVPGPLEIGFDYCFLLPTTNDRVPQVYVENHRVKNLDPDDPLWV
                     GNQKPDPDHPTGVSHRHTLRMDWSHGHNQSIHNGIGRIGFYTGGMKARFRDEDLADAW
                     VQQAQCWIEQNRNRPFFLFFASHDIHVPRMPHERFQGKTSLGYRGDAIIELDWCVEQL
                     LRTLDRLELAEKTLVVFCSDNGPVLDDGYKDGAVEKLGSHRPAGPFSGGKYSVLEGGT
                     RIPFITRWKGRITPRVSDEIVCTIDLAASFAALTGQTPAAGGCLDSFNVLDALLGEPR
                     ARGRDHLLQQSNDGTNLGLRVGDWKLVRLRNRDKSQATVTLEPHQPPAGTFGLYHLGR
                     DPGEQNDLSTQHPSELKRLQNLMETFTTDARSRAGNGSVEAFGRASADPSSAGNSARP
                     RTL"
BASE COUNT         1052 a         1759 c         2194 g         1136 t
ORIGIN      
        1 cacacggaga aagccggccg cgctggttga caggttgact tcgagttcgc gtccggagaa
       61 ccgcagcggc ctggtgatca gcgcgccgcc cttgtaggaa gcatgggcgg aggcgaaccc
      121 gtcggggcgc agtgtgaggc gttcgatgaa gatggacggt tgcccgtagt ggcggacgat
      181 gtagagagac atctcgatgg ggccggtcgg gaccacgccc aatgcggggt aattggcgcg
      241 agcgacccag ttgcgagggt cgggtccggg ccggatgaag ctctcgagga aggtccggtc
      301 atagcgcgtg ccgccgcggc tggtgagcag gacggcgtcg gaatcgtcgt tcttgaggcc
      361 gccgtaattg cgggggtttt ccagttcgag cgcctcgacc tgcgcgtcgg tgagagcgcg
      421 gcgccccggg ttgaatcgcg ccgcggtggc gatgtagatg tgcggcgccc ggaagtacgg
      481 ttgggtctgg tgggtgatgc agccgcagtt cgaggccctc gagccgctcg atcaggaacc
      541 ggtcgaccat caactcgcgc cgggtgccca ggtcaatcgg gtcctgggca ttgacgacac
      601 ttccggccgc caggagccag ccggccaaca acatcacgcg cgttctcatc agatcagatc
      661 ggatagtcaa gggcttccgg gatcatggtt tgggtcaggg cttcggcggc gcggtctttg
      721 gcgaggcggc agccggtgag gcatccgtcg agcttggtcc gcaggcgcag gaacggttcg
      781 aggccggcgc cggcggcgca gggaccggcc ttgcgcgggt gcgcgtcgcc gggctccgtg
      841 acatcggagc aggcgcgtgc ggagctttga atgccgggga tgatggcttc gagcgaggcg
      901 agttcatcct cgccgtaaca ggcgtggcaa gcctcggccg tccaggcagg gtggcggttg
      961 tagccgaaca cgatttcggc ggcgatgcgt ccggtctcgc cggctgcgcg ggcgctttgg
     1021 acgtggcaga ggtcggtgaa gaagcggacg aagcccggca acccttcggc gagtgccggg
     1081 ttagcggcgc cctgggtgtc gagttcgatg acatcgagga tgaactggcg ggcggcgagg
     1141 agccagcaga gggcgtcggc cagtgggaac gtcacgccct ggcgtgtctt gtgatagagc
     1201 ttgtcgccgt cggcatcggt cgccttctgc acgtgttcga gggtccagag ccagaggctc
     1261 atggcggtgg ccagggtgca ggccccggtg ccggggcggt ccgaggcgat ttggcgcatc
     1321 tcgacgatcc actgccggaa ctgggcgagg aagagtggat tggtcattgt gatcgagagc
     1381 tggaggcgct gcacggcctc cgggccctcg taggtggctt cgagctgggc gtccatccac
     1441 ttctgaccca ggaagccggg gcagtcttcc gtgacgccgt aaccgcccat gaggctgacg
     1501 gcctcgcgca tcatggtggc gccgtgtccg gtgttccata gcttgcaggc cgggcagagg
     1561 acgttggcca gcgcatcgag gaggacgaac cggaccaacg gttgatcgcg cagggcggcg
     1621 aagcgggcgc tgtcgcggcg gtcgtccggc tggccgcaaa gggcgagata ctcgagagcg
     1681 tcggtgtgcg tcgccgcgag ggccttgaac gcggcgcgtc cggtcagctt cttctcggcg
     1741 aggtaggcgt cgcgttgttt ttcgagcgga tcgagttcat cgaagattcg ggctgcggcg
     1801 aacccgagcg atgcgctggc ctcgccggcg gcccagatgt cgacaaggcg ctggacggta
     1861 tcctgcttct gttgaagacc gagttcgtaa cgcggcgtgc cgggcgcgcc ttcgccgccg
     1921 cggaaccggc gccgttggta ggccatgatc ggctcgattg cggacagcag tttggcggcg
     1981 gtcatgaggc ccacggtgac gcgggtgcgt cggaacacgg cttcgatcac ttcgctgtgc
     2041 gagtagttcg ggatgacgac gccgtccttg actgtgtacc cgcccacaat ccggtttgcc
     2101 ggcacgcgca ggctgaagac ggggtcggtg gtcgaggaga gttggtggac gagcttcttg
     2161 gtgggcgtgc cacggtccca ggtccccgga tcgccttctt cgaggatcac caggcaactg
     2221 cccttgatgc ggggatcgcc cgactcaacc gccgccgtca ccacgttcgc aaagcccatg
     2281 ttcgtgatga accggccccg cttctcgacc tggagaaccg gttcctggcc cgccggccag
     2341 tccgcaactc gcacccgccc ggccagcatt ccggtctcta ccccaacgaa tggcagaggc
     2401 tcggtcaggg caaaggcggc gcggacttgg cggcggtctt cacccggctg cacgggcatt
     2461 gcgccggcca gatagcgggc gcgctgctcg ggtgtgccgc gctcgtgaat cggcgcgagg
     2521 cccaggttgc cggcgaggct gcacgtcgcc gcgcccgcgt cgacccagga caactcgaat
     2581 gcgatcaagg ccagcgcgag attcttgggg ccctggatga acccgccgtg gtcgggatcg
     2641 agaaaggcgg cggtgatccc gctttcgtca aaggcaggca gcagggcggc cttttcgggc
     2701 gtccagtcgt gggcgttgcg ccctccgttt gcgacgaggc gggcaacggg tccgcgggcc
     2761 acctggcggg acgactggac gagcatctgc agatcatagc gatcggcgaa gcgccacatg
     2821 atctggcgca gatcgtctcc gggcagagtg cgcaggctat cggtcgacgg cgaggcagtg
     2881 cgagtttcca tgggctgatt ctaacggttg accgggtccg cgcacgccca aaaaaacgac
     2941 cggcccagcc gccacgcggc ggaggacacg gaccgcggct gcaaactcgg caacattgcc
     3001 gacactctgc aggctgatgc tcttcggcga ggcattctcg agacccttag ggtctgtggg
     3061 ctaaaaccac cgacttcggc agatttcgag cgcgtcgagc gtttcccccg gccagtagcg
     3121 gttggctggc agccggaagc tcccagatga acgtccgtaa ccccgcgaag ccgggaaagg
     3181 tcgcacggcg ctcgagcggg gagggcgcgc gcggattgcg ggacacgacc tcctggaggc
     3241 agcgctccgg gaggttaccc gccaggatgg gatttgggat gcggcggggc gcggcgcggt
     3301 ccgtggacat cgagacgacg cggccgaatt ggggtcaaac gagcgcgcgg ttgacgtagt
     3361 ggcgaatctc gaaatcggag tttgtctcga gggtctttgc cagatcgaac cgatcctcga
     3421 acggcgggaa gaatgtgtcg ccgtcgatgt cacgcaacac gagagtcagg tagaggtccg
     3481 agcatcgggg cagcgtctgc gcgtagattt ccgcgccgcc acagatgaag aaatcgcgcg
     3541 ggtccgaggc cggatcgagc gatgcgaggt cggacaccgt ccggacaccg gggaactgga
     3601 aacgggaacg gctcagaacg atggtctcgc gaccggggag cggtcggccg atcgactcga
     3661 aggtccggcg gcccatcacc agcacatgcc ccgtggtgag gcgcttgaac cagcggaaat
     3721 cgtccgggag atgccagggg atgcgatggt ctcggcctat gacgcggttg agcgccattg
     3781 ccgcaatggc cttgaaatgc ttcaactcgg ggtcggggtt gatttgaaca gaccgcaatg
     3841 gtgcgggctg gctgttaccc ggcgccctgc ccgcgccaga tcgactctct tatcagacac
     3901 gcgggtgttt taatgcctct tggagcgggg agacggacgg cgccaggaag cgaagccgca
     3961 gagcgctgcc gctgtcaaaa gggcgcagcc cgcgggttcg ggaaccagga tgatcgaagt
     4021 atcgatccgg aaattggatt gcgtggtgtg ccgctccgca aagaacaggt caaaatcgta
     4081 agttccaccg ggagtcaagc ccagtgtgtc aagattcacg gagccggaga tggcgccgtg
     4141 gacgcccccc aggtcaataa ccagtgactt attgatgaac acccagagat catcgtcacc
     4201 ggtgaactgg aacatctcgc ctccggcata ggtgaaactg gaatggagtt cgtaggtgaa
     4261 atggaagttg tggttgcgtc cctgattccc gaaaagctcg ttgtcgatcg gaaagaaatc
     4321 cgagtctgtg aaggtgtaca cattcggatc gggagtgatc gtgttgtcga gagtgagtga
     4381 caggggcgcg gcaaggttca cgccagcaac gttgttgtac cattggttga agttatctgc
     4441 tgtcgtaaaa cccgactttg ccggactcct ccagatcggt ttcttgtcga tccccaaggc
     4501 cgactcgacc tggccaggct ggtgtcctcc aatcgagcct tcgaaatcgg ggtgggtatc
     4561 gttgaagtca cggacggttc cggtcagggt gatggtggcg gcatgggcgc caatccagga
     4621 gagcagcatg cagcacccga cagtccggat gagccgtaag aagtgctgac gtttcatgtt
     4681 gattcttgga cgttcgattg ttcacctgtt gtttgtgttt ctgttccctt gaatattcgt
     4741 tttctaaacg caagccaaac gaaatctcag ttcccacatc acaacgtgcg aggccttgca
     4801 gaattcccag cactgctcgg gtccgcactg gctctgccaa acgcttcgac tgagccgttc
     4861 cctgcccggc tgcgagcgtc ggtcgtgaac gtttccatga gattctgcaa tcgcttcagc
     4921 tcggagggat gctgggtcga gagatcgttt tgttcgcccg ggtcgcgccc cagatggtag
     4981 agaccgaagg tgccggcggg cggttggtgg ggctcgagcg tgacggtggc ctggcttttg
     5041 tcgcggttcc tgaggcggac gagtttccag tctccgaccc ggaggcccag gttggtgccg
     5101 tcgttgctct gctggagcag gtgatcgcgt ccgcgggcgc gtggttcgcc cagcagcgcg
     5161 tccaggacat tgaagctgtc caggcagcca cccgccgcag gggtctggcc ggtgagcgcg
     5221 gcaaaactgg ccgcgagatc gatcgtacag acgatttcgt cggagacgcg cggggtgatg
     5281 cggcctttcc aacgggtgat aaacgggatg cgcgtgccgc cttcgagcac gctgtatttg
     5341 ccgccgctga aggggccggc gggccggtgg ctgcccagtt tctcgacggc gccgtccttg
     5401 tagccgtcat cgagcacggg tccgttgtcg gagcagaaca ccacgagcgt cttctccgcc
     5461 aattcgaggc ggtcgagggt tctcagcaat tgctcgacac accagtcgag ctcgatgatc
     5521 gcgtcgccgc gatagccgag gctggtcttt ccctggaacc gctcatgcgg cattcgcggg
     5581 acatggatgt cgtgcgaggc gaagaacagg aagaaggggc gattgcggtt ctgctcgatc
     5641 cagcactggg cctgttgaac ccaggcatcg gcgaggtctt cgtcccggaa gcgcgccttc
     5701 atgccgccgg tgtagaatcc gatgcgtccg atcccgttgt ggatgctctg gttgtgtcca
     5761 tgagaccagt ccatccggag cgtgtgtcga tggctcacgc ctgtggggtg atcggggtcg
     5821 ggtttctggt tgcccaccca caggggatcg tccgggtcga ggttttttac ccggtgattc
     5881 tcgacataga cctgggggac gcggtcgttg gtggttggca gcaggaaaca gtaatcgaat
     5941 ccgatctcga gcgggccggg cacgagttcg ccattccaat cgggtgccgg ggcgccgagg
     6001 ccgaggtgcc atttcccgat cacagccgtt gtgtatccgg cgcgtttgag gatggctggc
     6061 agagtctcgg tgccgggctt gatcagggcc gggccgttgg gcggggcgat accggttccc
     6121 ttctggcgga acgcgtacgt g
//
LOCUS       DAOQRO010000208         6129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12913_length_6129_cov_3.869773, whole genome shotgun sequence.
ACCESSION   DAOQRO010000208 DAOQRO010000000
VERSION     DAOQRO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_6129_cov_3.869773"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKK20_08575"
     CDS             complement(<1..221)
                     /locus_tag="PKK20_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99982.1"
                     /translation="MNPIALRRASLGLGWLALSGAAALCGDRLSLEGAWRLQLDRADV
                     GIAEHWFERRLSDTVRLPGSLPGQGIGDPV"
     gene            421..951
                     /locus_tag="PKK20_08580"
     CDS             421..951
                     /locus_tag="PKK20_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNU99983.1"
                     /translation="MQPFIRFVGIINITVWLGAAVFFTVAAGPAFFSAEMLSFLEERF
                     ARRTAEVIVQRYLLLQQWCAAIALLHVLVEYLYSGRQVDRWCLGLLSVIFLVALIGSY
                     WVLPHTHELHRIMYSVTSTVAQQESARSRFALIHGASYFVNFLIIAGLIYYLWRLTRL
                     GQTTRFGFGGADKFRA"
     gene            1086..1325
                     /locus_tag="PKK20_08585"
                     /pseudo
     CDS             1086..1325
                     /locus_tag="PKK20_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
     gene            complement(1408..1812)
                     /gene="rsfS"
                     /locus_tag="PKK20_08590"
     CDS             complement(1408..1812)
                     /gene="rsfS"
                     /locus_tag="PKK20_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNU99984.1"
                     /translation="MDPRKLALQCRRLADAKKAENIVILDVRKLSSVTDYFVIATGTS
                     EPHLRALMAEITEKLREGFDVRPHATDGTPRNNWIVLDYFDVIVHLMRDDVREHYGLE
                     SLWGDAPRLRPLRLRSASAVRPRTARDSRATR"
     gene            complement(1840..2433)
                     /gene="nadD"
                     /locus_tag="PKK20_08595"
     CDS             complement(1840..2433)
                     /gene="nadD"
                     /locus_tag="PKK20_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNU99985.1"
                     /translation="MSAPNALRRIGLFGGSFNPVHLAHLVVADAAFEELELDRLFFIP
                     AAQSPFKQGIALAPAALRLRMLRLALAGQSHAEVDEQEIRRGGVSYAIDTVKDYARRY
                     PMAELFYLIGADHVAQLSKWRDASGLAGLVQFAVIPRPDEPPVAAPAAFRVRCLRGVP
                     VGVSASQIRGRVAAGLSIRWIVPSGVDEVIRQHRLYL"
     gene            complement(2603..6031)
                     /locus_tag="PKK20_08600"
     CDS             complement(2603..6031)
                     /locus_tag="PKK20_08600"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNU99986.1"
                     /translation="MRCLIAALAVVTLLPWLCGFGALAGDRLAESFIRPDAETRPWVY
                     WYWIDEHISREGITRDLEAMARVGIGEALIGHVSPGRERGPVHMLSDRWWEMVEHAVF
                     EGQRLGVDIGFFNSPGWSQSGGPWITADQAMRYVVQTETRVRGPAQLEGVLPAPEGEY
                     RDVAVLAFPMPAEQEAPILVRQPLPETPGASPGVERMFDGDTNTAFVFPRGTGTDRRW
                     QVDLVLEGDRPVQGVSLHPVEHPFQVRVELHVSTDGRHYEPLRRIHFDRRRTMWNLGP
                     MTWAPLTLACPATAARHFRLVFSELDAPGDAGFREIVFTGRPTLDYLVEKQLGKMYPE
                     PLPGWDAYLWPPQAEPTSTLALGDVRNITGLMHAGGRLRWDVPEGEWVILRCGTLPTG
                     AVNEPVPAEAKGYEVDKMSREGSRHHFASYIGAFLDRVPPERRRALRHVVIDSYEVGS
                     QNWTPGLERVFVERLGYDPVPWLPVLSGRVIGSADLSNRFLWDLRRLVADLIAENYLA
                     AFREAVNARGLKLWIENYGHWGFPAEFLQYGGQADIVSGEFWYRNHLWDLGSIELRGA
                     SSAAHTYGKPITSAEAFTAGFNFRQSPAAMKSRGDWAFTQGINHFVLHLYIHQPWEEQ
                     KPGVSAWFGMSYQRHNTWFEQSRSWIDYLRRCHFLLQQGSPTPDLLYFIGEDTPKMTG
                     ILDPPLPPGYDFDFVNAEVIQKRLSVKDGLLTLPEGVQYRLLVLPPLDTMRPELLAKI
                     GQLVRAGAVVVGPPPRRSPSLSGYPCADAEVERRADEIWGPCDGVQATEHRHGKGTVF
                     HGVAPADVLTRLGAVPDVVCGDSFVLWTHRRGKGFDVYFISNQSDQARELEIAFRVGA
                     RIPEFWCPVSGDIRRIVRFKTAGDYTHVTVPLGGHGSLFVVFRERSRGDQVVDLSPTP
                     PGLSDVGEAAVQAAVCYARDNREFVATRARIACQVRRANGRSAALDSGPVPGPLEVAG
                     SWTLCFPPGWDCPDRIVLERLMDWRESPVEGVRHFSGTARYRTVLDIPEEYLAGGDRL
                     WLDLGAVATIAAVKVNGQALGVLWCSPYAVDITGAARAGANELEIEVTNLWWNRLVGD
                     AKYPNGFPDGRGGFTGDAPKTFTTHKAWGPREELEPSGLLGPVRVHIERHVQLP"
BASE COUNT         1160 a         2090 c         1840 g         1039 t
ORIGIN      
        1 acaggatccc cgatgccttg tcccggcaat gaaccgggca aacgcacggt gtcggaaagc
       61 ctccgctcga accaatgctc ggctatcccg acgtccgcac ggtccaactg caaccgccag
      121 gcgccttcaa gcgagagacg atcgccgcaa agcgccgccg cgcccgagag cgcaagccag
      181 cctaacccca gggaggcgcg cctcagagca atcggattca taagccgtga tatcaggtgt
      241 cgaaatggca tcggggcgat cacaggcaag acactatcgg ggcggaccga cgaacgcaac
      301 cttcgccctg agtcgtcacc cttgagggtg aaaccgcctc agcaggcgag cgccgggtca
      361 gaataagaac cgaggattcg cgacttggca tgggagcggg actcgagtta tgatgccacc
      421 gtgcagccat tcatccgatt cgttggcatt atcaacatca cggtgtggtt gggtgcggcg
      481 gtctttttca ccgtggcggc tggaccggcg ttcttctcgg ccgagatgct cagtttcctc
      541 gaggaacgct tcgcgcggcg cacggcggag gtgatcgtcc agcgctactt gcttcttcaa
      601 cagtggtgcg ccgcaatcgc gctgctgcac gttctggtcg agtatctcta ttcggggcgg
      661 caggtcgacc gttggtgtct tggattgttg tcagtcatct tcctggtcgc gttgatcggc
      721 agctactggg tgctgcctca cacacacgag ttgcaccgga tcatgtactc ggtcacgtcc
      781 accgtcgcgc agcaggagtc tgccagaagc cgcttcgccc tcatccacgg cgcctcgtat
      841 ttcgtcaact tcctgatcat tgctggcttg atctactatc tctggcgcct cacccgactc
      901 ggacagacga cgcggttcgg atttggaggg gcggacaaat tccgcgcttg acaaattcgg
      961 gcacgtggct gacacaaccg cctgtaatcc aacggtaaga ccacccgatg ggagatgcta
     1021 tgacgcatca cgattgttcg cgagaaccgg gccagcacga tctcgcatcc ggccccaaag
     1081 cgcacggcga ggaaaaaacc ctccggagcg agcgactgca gatcgagcgt aagacgttcc
     1141 tgctcgcgct taaggaaaat ccccgggggc agttcctgcg aatcacagag gatgtcggcg
     1201 gacgccgcga caccatcatc attcctgcct cgggcctcga ggagttcctc aaagccctcc
     1261 aggccatgat cgaccagatc gaccccgccc ccgcactgac cgcttcgatc aagtccgcgg
     1321 attagccgct ccctgatccc gcactgtccc ggcgccgtgt caacggcgac ttcgcctgga
     1381 cgccagcgcc gccagcgatg acgacggtca gcgggtggcc cggctgtccc gcgcggtgcg
     1441 cgggcggacg gctgaagccg accgaagccg caacggccgc aggcgcggtg cgtcccccca
     1501 aaggctctcg aggccgtagt gctcccgcac gtcatcccgc atgagatgca cgatgacatc
     1561 gaagtaatcg agaactatcc agttgttcct gggcgtgcca tcggtcgcgt gaggacgcac
     1621 gtcgaacccc tcacgaagct tctccgtgat ctcggccatc agggcgcgta aatgcggttc
     1681 gctggtgccg gtggcaatca cgaaatagtc cgtgacgctc gacaacttgc gaacatccag
     1741 gatcacgatg ttctcggctt tcttggcgtc cgccagtctt cggcattgca gggcgagttt
     1801 gcgagggtcc atgacaggag aaacagagac taccatgcgt tagagataaa gccggtgctg
     1861 acgaatcact tcgtcaacac ccgacggaac aatccagcga atcgaaagtc cggccgccac
     1921 ccggccgcgg atttgcgacg cggacacgcc aaccggcact ccgcgcaggc aacgcacacg
     1981 aaaggctgcc ggcgccgcga ccggcggttc gtcgggacgc ggaatcaccg cgaactgcac
     2041 cagccccgcc aggcccgagg cgtcccgcca cttggacaac tgcgccacgt ggtccgcgcc
     2101 gatcaaatag aataactccg ccatcggata tcggcgcgcg tagtccttca cggtgtcgat
     2161 ggcgtacgac acgcctccac gccggatctc ctgctcatcc acctccgcgt ggctctggcc
     2221 ggccagggcc agccgcaaca tgcggagccg cagcgctgcc ggggccaggg caatgccctg
     2281 cttgaaaggc gactgcgccg ccgggatgaa aaagagacga tcgagttcca gttcctcgaa
     2341 cgccgcatcc gcgaccacaa gatgggcgag gtggacggga ttgaacgatc ctccaaacaa
     2401 accgatacgc cgtagggcat taggggcact catcggccgg ccaatgaggc tgcccggtag
     2461 gcggctggta ctgccatgtc tgacacgccg gcaactatgc cccatacccg cccggtttca
     2521 agccgcttct tcaaaccgtc gcttccggac acctcgactc tccatttgtc aattatttag
     2581 atgcgacccc attgccctct tttcagggca attgcacgtg ccgctcgata tgcacccgca
     2641 ctggccccag cagcccggac ggctcgagtt cttcacgcgg cccccatgcc ttgtgtgtcg
     2701 taaaggtctt tggggcgtcc cccgtgaagc cgccccggcc atcggggaat ccattcggat
     2761 atttcgcatc acccacaagg cggttccacc agaggttggt cacttcgatc tctagctcat
     2821 tcgcgccggc acgcgccgcg ccggtgatgt ccaccgcgta cggagagcac cacagcaccc
     2881 ccagcgcctg cccattgacc ttgaccgcgg cgatcgttgc caccgcgccc aggtccaacc
     2941 acaagcggtc gccgccggcc aagtactcct ccgggatgtc cagcacggtc cggtaccggg
     3001 ccgtcccgga gaaatgacgc accccctcga ccggggattc ccgccaatcc atcagcctct
     3061 cgagcacgat gcggtccgga cagtcccagc ccggcggaaa gcagagggtc caactgcccg
     3121 ccacctcgag cggcccgggc accggcccgg aatcgagcgc cgcgctgcgc ccgtttgccc
     3181 tcctaacctg acaggcgatt cgggcgcggg tggcaacaaa ctcgcggttg tccctcgcgt
     3241 agcacacggc ggcctggacc gccgcttcac cgacgtcgga cagacccggt ggcgtcggag
     3301 acagatcgac gacctggtcg ccacggctgc gttcgcgaaa caccacaaac agggagccat
     3361 gaccgccaag cggcaccgtg acgtgcgtgt aatcgcccgc ggttttgaac cggacgattc
     3421 gccggatatc gccgctcacc ggacaccaga actctggaat cctggcgccg acccggaagg
     3481 cgatttccag ttctcgcgcc tggtcggatt ggttggaaat gaaatacacg tcgaaaccct
     3541 tcccgcgccg gtgtgtccag agaacgaacg agtcgccgca caccacgtcg gggacggcgc
     3601 ccagccgcgt cagcacatct gccggcgcga cgccatgaaa gaccgtgccc ttcccgtggc
     3661 gatgctcggt ggcctgcacg ccgtcgcacg gaccccagat ctcgtccgcc ctgcgctcga
     3721 cctcggcgtc agcgcacgga taccccgaga ggctgggcga gcgtcgtggc ggcggcccca
     3781 cgaccacggc gccggcgcgc accaattggc cgatcttcgc cagcagttcc gggcgcattg
     3841 tgtccagagg cggcaacacc agcagacggt actgcacgcc ttccgggaga gtgagcagac
     3901 catcctttac cgagagccgc ttctggatga cctcggcgtt cacgaaatcg aagtcatacc
     3961 cgggcggcag cgggggatcg aggatgccgg tcatcttggg cgtgtcttcg ccgatgaagt
     4021 agagcaggtc gggcgttggg ctgccctgtt gcagcaggaa gtggcagcgg cgcaggtaat
     4081 cgatccagga gcggctctgc tcgaaccagg tgttgtgccg ctgatagctc atgccgaacc
     4141 aggcgctcac tcccggcttc tgctcttccc agggctgatg gatatagagg tgcagcacaa
     4201 aatggttgat gccctgagtg aaggcccagt cgccccggct cttcatcgcc gccggcgact
     4261 ggcggaagtt gaagcccgcc gtgaacgcct cggcggatgt gatcggcttg ccataggtgt
     4321 gcgccgccga ggatgccccg cgcaattcga tgctgcccag gtcccagaga tgattgcggt
     4381 accagaattc gcccgagacg atgtcggcct ggccgccgta ctgcagaaac tccgccggaa
     4441 aaccccagtg accgtaattc tctatccaga gcttcagccc ccgggcattg accgcctccc
     4501 gaaacgccgc cagatagttc tcagcgatca ggtcggccac caggcgccgc aggtcccaga
     4561 gaaatcggtt cgatagatcg gcgctgccta tgacccgacc actgagcacc ggcagccagg
     4621 gaacaggatc gtagcccagg cgctcgacga acacccgttc gagcccgggc gtccagtttt
     4681 gcgatcccac ttcgtagctg tcgatcacca cgtgccggag tgcgcgccgc cgctcgggcg
     4741 gcacgcggtc gagaaaggcg ccgatgtagg aggcaaaatg atggcgactg ccctcccggc
     4801 tcatcttgtc gacctcatac cctttggctt ccgcaggcac aggctcgttg accgcccccg
     4861 taggcaaggt gccgcaccgc aggatgaccc actcgccctc cggcacatcc caacgcaacc
     4921 gccccccggc atgcatcagg ccggtgatgt tcctgacgtc tcccagggcc agagtcgaag
     4981 tcggttccgc ctgcggcggc caaaggtacg catcccagcc gggcagcggc tcgggataca
     5041 tcttccccaa ctgtttctcg acgaggtaat cgagagtggg cctgccggtg aaaacgatct
     5101 ctcgaaagcc ggcatcgccc ggcgcatcga gttcgctgaa caccaggcgg aaatgccttg
     5161 ccgccgtcgc gggacaggcc agcgtcaggg gcgcccaggt catggggccg aggttccaca
     5221 tggtgcgacg gcggtcgaag tggatgcgcc gcagcggttc ataatggcga ccatcggtgg
     5281 acacatgcaa ttccacccgc acctgaaagg gatgttcgac ggggtgcagt gaaacgccct
     5341 gcaccggacg atctccctcg agcacgagat ccacctgcca acgcctatca gtgcccgttc
     5401 cccgcggaaa gacgaacgcg gtgtttgtgt cgccgtcgaa catgcgctcg acaccggggg
     5461 aagcgcctgg tgtttcaggc agtggttgcc ggaccaggat gggggcctcc tgctctgccg
     5521 gcatcggaaa ggccagcacc gccacgtccc gatattcgcc ctccggcgcg ggcaggaccc
     5581 cttccaactg cgctgggccg cgcacgcgcg tctcggtctg caccacgtag cgcatagcct
     5641 ggtcggccgt gatccacggc cctccggatt gactccaccc ggggctgttg aagaacccga
     5701 tgtcgacacc cagccgttgc ccctcgaaca ccgcgtgctc gaccatctcc caccagcgat
     5761 cgctcagcat gtgcaccgga ccgcgctccc ggcccggcga gacgtgcccg atcagggcct
     5821 cgccgatgcc cacccgggcc atcgcctcca ggtcccgggt gatgccttct cgcgagatat
     5881 gctcatcgat ccagtaccag tacacccacg gacgcgtctc cgcgtccggt cggatgaagc
     5941 tctccgccag gcgatcccct gccagggctc cgaagccgca gagccagggc agcagggtca
     6001 cgacggccag ggccgcgatc agacatctca taagccttgg ggtcacatct tcatttttga
     6061 caattgctcg ctgaccgcgt cgatccgctg ccgccatgcc ttgtctctgt gaatctggcg
     6121 tcgaacccg
//
LOCUS       DAOQRO010000209         6126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12920_length_6126_cov_5.139680, whole genome shotgun sequence.
ACCESSION   DAOQRO010000209 DAOQRO010000000
VERSION     DAOQRO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12920_length_6126_cov_5.139680"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKK20_08605"
     CDS             <1..328
                     /locus_tag="PKK20_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU99987.1"
                     /translation="PLDALSWDHQTLEPRGTCVANTLLTAIKSATAERWLKAAESAGR
                     RVDVLDAGPAALANAHKQSQHRPTHLPRRELESGVAHYGHGIGVWARYQQGQFIGGAP
                     THAVCT"
     gene            487..1779
                     /locus_tag="PKK20_08610"
     CDS             487..1779
                     /locus_tag="PKK20_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU99988.1"
                     /translation="MRTASGGRLPALLAALVGLAVAPGQRASSLDWPNWRGPDANGST
                     ATGKYPTRWDATNVLWKAVLPGKGSSTPIALDRRIYVTAPADGQDTLLAFDLDGTALW
                     QTKLGTESRPKHRTLGSSANASPVTDGKGLFVYYRSGTFAALELDGTVRWILNLVEQF
                     GQDQLFWDQGSSPVITDKEVVMVRMHGGDSWLAGFDKATGELRWRQSRNFETPVENNN
                     AYNTPVPHQHAGKPALLVWGADHLTAHDAANGKTLWSCGGFNPDATGYWPAIATPVIV
                     GDLAVVPVGRDDRNGQSRVHGIKLGGEGDVTATHRLWKRKDLGVFVSSPAVYKDRIYL
                     LRHRGSVVCLDPATGQTVWSDALPEDKSPYYASPTIANGILYAAREDGMVFAVRVEER
                     FELLGANPMGERIVASPVPVANRLLLRGDRHLFCVAGD"
     gene            complement(1876..3522)
                     /locus_tag="PKK20_08615"
     CDS             complement(1876..3522)
                     /locus_tag="PKK20_08615"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNU99989.1"
                     /translation="MTGILSAAHGASSDTQFLRLPVKEYRDKMKGGWIGQIVGVSWGG
                     PTEFKWQDKIIPADQMPAWRPSMINDAFDQDDLYVEMTFLRSLEEYGIDVSIRQAGID
                     FANSGYPLWCANNAGRTNLRRGIAPPDSSHPQFNKCPNDIDYQIEADYSGLIAPGMPQ
                     VAIDLGEKFGRLMNYGDGVYAGQFMGALYAAAFFESDPVKLAQTALAAIPAQSQYAEM
                     VRDMIAWYQADPNDWEKTWWLCQKKYREDKEYQKASNGGIDCKINGAYVLMGLLFGRR
                     DLDQTILISTRSGMDSDCNPSSSAGVLFTTIGFSKLPPRFNTGLDETREFSHTAYNFP
                     RLIDVCEKLARQFLVRYGGRIEKDGAGEDVFVIPVRAPKPSALELSWAPGPIANSRFT
                     PEEMAKITASNTPKRAIEAVEKLFPGWKLTDCGQDMDPGLRAEWQGRKNVVATHPLDR
                     ETGCKLTRTIDVPAGRTTRLHLVVGHDPQGDFDLIVRAAGEELVRKAVSPATCTQGHW
                     LVQDVDLSRFAGRKVEIELINQPSGWAYEAAYWAEITVKD"
     gene            complement(4051..4407)
                     /locus_tag="PKK20_08620"
                     /pseudo
     CDS             complement(4051..4407)
                     /locus_tag="PKK20_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            4605..5567
                     /locus_tag="PKK20_08625"
     CDS             4605..5567
                     /locus_tag="PKK20_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNU99990.1"
                     /translation="MNKPSPALNRRSFLTAGAASVALAAAGAAPATRGAESESGRIVT
                     PPPGKRLLLACKLGMIASEAAGRKLTVVERLRMAAEAGFDGVDFDQAGEYTPEQARDA
                     VRQSGVFVHNAINHAHWEKRLTSASAEDRARSRANIEHCLRVSHAAGGSGVLIVLGKG
                     DDGPAEVIEERCRQELKQLLPLAASLGQPILIENVWNQMFYQHDGPPDQSPDQFIKFV
                     DTLRSPWIGMYFDIGNHWKYAQPADWIRAFGHRCVKLDVKGFSRTRNKFVDITAPDDD
                     LPWDQVRKALEDIGYCGWATAEVGGGDVKRLTQVREQMQKAFGL"
     gene            complement(5564..>6126)
                     /locus_tag="PKK20_08630"
     CDS             complement(5564..>6126)
                     /locus_tag="PKK20_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNU99991.1"
                     /translation="RGVRLFDGRTLDGWEGDTNTTWRVVEGALVGGSLKAPVPRNEFL
                     CTARAYTNFVLKVKFKILGPGANAGIQVRSRRIPDHHEMIGYQADLGDPEWWGCLYDE
                     SRRNKVLAKADKERVDKVLKRGDWNEYVIRCEGRRVQLWINGLQTVDYTETDQSIEQA
                     GLIGLQIHGGPPSEAWYKDILLEYLD"
BASE COUNT         1174 a         2005 c         1850 g         1097 t
ORIGIN      
        1 ccccctcgat gccctgagct gggatcacca aacgctcgaa ccgcggggaa cctgcgtggc
       61 caacaccctt ctcaccgcga tcaagtccgc cacggccgag cgatggctga aagcggccga
      121 atccgcggga cggcgtgtcg acgtgctcga tgccggaccg gcggcgctgg cgaatgccca
      181 caaacaaagt cagcaccgtc caactcattt gccgcgccgt gagctggaat cgggtgtcgc
      241 ccactacgga cacggaattg gcgtatgggc tcgctaccaa cagggccaat tcataggcgg
      301 agcgccgaca catgccgtat gcacttgaca tgccggggga ctagccgtcc ccagtccgac
      361 cttctcgact tcatgaattg gcgctgcgcg accaagacgg caccgccttt tcgtttggcg
      421 gatgggacca gacggactat gtttgccgcc gttttacaga caatgcacga aatgacacca
      481 gaggtaatgc gcacagcgag tggcggtcgg ttgccggctc ttttggcggc cctcgtcggg
      541 ctcgccgtcg cgccaggaca gagagccagc tcgctcgatt ggccaaactg gcgcggacca
      601 gacgccaatg gcagcacagc aacagggaag tatcccaccc ggtgggacgc gaccaatgtt
      661 ctctggaaag cggtgttgcc gggcaaaggc agctcgacgc ccattgcgct tgatcgccgc
      721 atctacgtga cggcgcccgc cgatggacaa gacacactgc tggcgttcga cttggacggc
      781 acagcgttgt ggcagaccaa gctcggcacc gagagccgcc cgaagcaccg gacgttggga
      841 tccagcgcca atgcctcgcc ggtgacggat gggaagggac tcttcgtcta ctaccggagc
      901 ggcaccttcg cagccctcga gttggatggg accgtgcgct ggatactcaa cctcgtcgaa
      961 cagttcggtc aagaccaact cttctgggac cagggctcct caccggtgat cacggacaag
     1021 gaagtggtca tggtccgcat gcacgggggc gactcgtggc tcgccggctt cgacaaggcc
     1081 accggggaac tgcggtggcg gcaaagccgg aattttgaga cgcccgtcga gaacaacaac
     1141 gcctacaaca cgcccgtgcc gcaccagcac gccggcaaac cagccctcct tgtctggggc
     1201 gccgatcacc tgaccgccca cgatgccgcc aacggcaaga cactctggtc ctgcggcggc
     1261 ttcaatccgg acgccaccgg ctattggccc gcgatcgcca caccggtgat tgtgggggac
     1321 ctggcggtcg tcccggtcgg gcgcgatgat cggaacgggc aatcgcgcgt gcatggcatc
     1381 aagctcggcg gtgaaggcga cgtgacagcc acccatcgcc tctggaaacg caaagacctc
     1441 ggcgtctttg tcagcagccc ggcagtctac aaagaccgaa tctacctctt gcgccatcgg
     1501 ggcagtgtcg tgtgcctgga ccccgccacg ggccagacgg tttggagcga cgctttgccc
     1561 gaagacaagt ccccctacta cgcgtcgccg acgatcgcca acgggattct ctatgccgcc
     1621 cgtgaggatg gcatggtttt cgccgtccgg gtggaagaac gcttcgagtt gttgggcgca
     1681 aacccaatgg gcgaacgcat cgtggcctcg cccgtgcccg tggccaatcg cctgttgctc
     1741 cgtggcgacc gccacctgtt ctgcgtggcg ggagactgat tagtgagaga aacaaaaccg
     1801 cgcccggaaa ggaatccagg ggcattctgt cggtcctgaa cgacgacgtg ccgcgtcagt
     1861 ctgagcggag ccttgctaat ccttcaccgt gatctcggcc caataggcag cctcataggc
     1921 ccatccgctg ggctggttga tcaactcgat ctcgaccttc cgacccgcaa accgactcaa
     1981 gtccacgtcc tggacaagcc aatgcccctg ggtgcaggtc gccgggctca ccgccttgcg
     2041 caccaactcc tcgccggcag cccgcacgat cagatcgaag tcgccctgcg gatcgtgacc
     2101 aaccaccagg tgcagccggg tcgtgcggcc cgccggcacg tcgatcgtgc gcgtgagttt
     2161 gcagccggtc tcccgatcga gcggatgcgt cgcaaccacg ttctttcgac cctgccattc
     2221 ggcgcgcagg ccgggatcca tgtcctggcc gcaatcggtc agcttccatc ccggaaagag
     2281 cttctcgacc gcctcgatcg cgcgtttggg cgtgtttgaa gccgtgatct tcgccatctc
     2341 ctccggagtg aaccggctgt tggcgatcgg ccccggcgcc cagctcaatt caagcgcgct
     2401 cggcttgggc gcgcgcaccg ggatgacaaa gacatcctcg ccggcgccgt ccttctcgat
     2461 ccgaccgccg tagcgcacga ggaactggcg cgccagtttc tcgcacacat cgatgagccg
     2521 cgggaaattg tacgccgtgt ggctgaactc gcgcgtctcg tccagccccg tattgaaacg
     2581 cggcggcagc ttcgagaagc cgatggtggt gaacaggaca cccgccgaac tcgacggatt
     2641 acagtccgag tccatgccgc ttcgagtcga gatgaggatc gtctggtcca aatcgcgtcg
     2701 gccaaagaga aggcccatca ggacgtaggc gccgttgatt ttgcagtcga tgccgccatt
     2761 cgatgccttc tgatactcct tgtcctcgcg gtatttcttc tggcacagcc accaggtctt
     2821 ctcccaatcg ttcgggtcgg cctggtacca cgcaatcatg tcccgcacca tctccgcgta
     2881 ttggctctgc gccggaatcg cggcgagcgc tgtctgggcg agcttgaccg gatcggactc
     2941 gaagaacgcc gcggcataaa gggcgcccat gaactggccg gcgtacacgc cgtccccgta
     3001 gttcatcagc cgtccgaact tctcgcccaa atcgatggca acctgaggca tgcccggcgc
     3061 gatcagcccc gaataatccg cctcgatctg ataatcgatg tcgtttggac acttgttgaa
     3121 ttgcggatgg ctcgaatcgg gaggggcgat gccccggcgc agattcgtgc gcccggcatt
     3181 gttggcgcac cagagcggat acccgctgtt ggcgaagtcg atccccgcct gccgtatgga
     3241 aacgtcgatc ccgtattcct cgagcgaacg caggaacgtc atctcgacgt agagatcgtc
     3301 ctggtcaaac gcgtcgttga tcatcgacgg acgccatgcc ggcatttgat cggcgggaat
     3361 gatcttgtcc tgccacttga actcggtggg gccaccccac gacacgccga caatctggcc
     3421 gatccagccg cctttcatct tgtcgcggta ttctttgacc ggcagtcgca agaactgggt
     3481 gtcgctcgaa gcgccatgcg cggcacttag aataccggtc accaacagga gtccaagtgc
     3541 ctgctgcgcc ttaaagacaa agcgcgtccg agcgccacgc cttactgagg gatcacccgg
     3601 atgcacccca tcgccaccct cacctgcccc gcatagcgga cccccctctc ccatctgcct
     3661 gcgcccaggc ttcggcaggc aggtcggatg ggagttggac agggtgattg gtctgtgtcg
     3721 tgaatccaac cgcccaggca gcaaacaggc cggggtcgtg gtgctgagaa cagcgagaga
     3781 aaacagggca ttcattcgtg tgaaggcgtc agggcctcgg tctcattgga ctcatcagca
     3841 gtgacaccag tgtcgccgtt gactccaggg accgggcggg tcgattggac aagctgtggg
     3901 cgcgtgtgct cactttgacc gggtcagaat agcttggcgc ctcgacgctg aaaagcgaaa
     3961 agcgagtcga cgcaaccaga gcactgaccc tccgaacggc atcgaccaag gcaggaggag
     4021 cgcatcgcag tgttcgatgc aaccctgctg tcactcgacg gagttccgca acacgccaat
     4081 cccctcgatt tccacttcaa caacgtcgcc cggcttgagc gccgaggtgg ccccgggcgt
     4141 cccggtgaaa atgagatcgc cgggctgaag cgtgacgtac cggctgatca tgctcacgat
     4201 gccggccgcg tcgtgaatga gtcggctggt ccggtcctgt tgcttcactt cgccgttgac
     4261 gcgcacgcgc aactcgaggt tgctccaatc cagtccggtg acgatccagg ggccgaccgg
     4321 accgaacgtg tcactccctt tggctcgcca ccattggatg tccttatcct gttcgtcgtt
     4381 ctgccagtag cgctcgctga cgtcattgca ttcatgtgca ttgttggacg gatgaaacca
     4441 tttcctcacg aatggaggca acagccgatt cgcgcatacc accacctttc gccattgcat
     4501 cgcggcagca atccgggcaa gaatcgcacc gtgttctgca ccacaacatc atgcggcttg
     4561 aaatcgccct cgaaccctcg aaccctaagt cccggaggtc atccatgaat aaaccgtcac
     4621 ctgcactgaa tcgccgcagt ttcctcaccg ccggagccgc gtcggtcgct ctcgcggccg
     4681 ccggagccgc gcccgccacg cgcggggcgg agagcgagag tggacggatc gtcacgccgc
     4741 ccccgggcaa acggctgttg ctcgcgtgca aactggggat gattgcgtcc gaggccgcgg
     4801 gccggaagct cacggtggtc gagcgcctgc ggatggccgc ggaggcgggc ttcgacggcg
     4861 ttgatttcga tcaggccggc gagtacacgc ccgaacaggc gcgcgacgcg gtgcggcaat
     4921 cgggggtctt cgtccacaat gcgatcaatc acgcccattg ggagaagcgc ttgaccagcg
     4981 ccagcgcgga ggaccgcgct cgcagccggg ccaacatcga gcattgcctg cgcgtgtcgc
     5041 acgcggccgg cggcagcggc gtgcttatcg tgctcggcaa gggcgacgac ggcccggcgg
     5101 aagtcatcga ggagcgctgc cgccaggaac tgaagcaatt gcttccgctt gccgcatcgc
     5161 tcggacagcc gatcctgatc gagaacgtgt ggaaccagat gttctatcaa cacgacgggc
     5221 cgccggatca aagcccagac cagttcatca agttcgtcga caccctccga agcccgtgga
     5281 tcggcatgta cttcgacatc ggcaatcatt ggaaatacgc ccagccggcg gactggattc
     5341 gcgccttcgg ccaccggtgc gtcaaactgg acgtgaaagg attcagccgg acgcgcaaca
     5401 aattcgtcga tatcaccgcg cccgatgacg acctcccgtg ggatcaggtc cgcaaggcgc
     5461 tcgaagacat cggctactgc ggctgggcga ccgcggaagt gggcggcggc gacgtcaagc
     5521 gcctgacgca agtgcgcgag cagatgcaga aggcattcgg actctaatcc agatactcca
     5581 agaggatgtc cttgtaccag gcctcgctcg gcgggccgcc gtgaatctgg agaccgatca
     5641 gacccgcttg ctcgatgctc tgatcggttt ccgtgtagtc gacggtctgc aatccgttga
     5701 tccagagttg aacccgccga ccctcgcagc gaatgacgta ctcgttccag tcgccgcgct
     5761 tgagcacttt atccacccgc tccttgtccg ccttggccag cactttgttc cgacgcgatt
     5821 catcgtagag acagccccac cactccggat cgcccagatc ggcctgataa ccgatcatct
     5881 cgtggtggtc cggaatcctg cgcgagcgga cctggatgcc ggcattggcg ccgggcccaa
     5941 gaatcttgaa cttcaccttc agcacgaagt tcgtgtaagc gcgcgcggtg cacagaaact
     6001 cgttgcgcgg cacgggcgct ttcagcgagc cgcccaccaa tgcaccctcg acaacccgcc
     6061 atgtcgtgtt ggtgtccccc tcccatccgt caagggtgcg cccgtcgaac aggcggacgc
     6121 cccggg
//
LOCUS       DAOQRO010000210         6118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_12948_length_6118_cov_4.841992, whole genome shotgun sequence.
ACCESSION   DAOQRO010000210 DAOQRO010000000
VERSION     DAOQRO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12948_length_6118_cov_4.841992"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..1440)
                     /locus_tag="PKK20_08635"
     CDS             complement(403..1440)
                     /locus_tag="PKK20_08635"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNU99992.1"
                     /translation="MKVAVVGCGAVGSFYGAKLCRAGLPVFFLLRSDYEVVRQEGVRI
                     ISPDGEFRAFPCPARQPAEIGVCDLVIIALKATANHAFPSLLPPLVGPQSWILTLQNG
                     LGNEEALAALFGPDHVLGGLCFVCLNRIAPGVVRHTAHGQIVLGQYCQAPTDGARAVA
                     QCLIRAGIHCDMTPDLAQAHWEKLVWNIPFNGLGVAGIAGIQNVLEGRLPRTLDRVTT
                     LATDELLRDPRWTALLRQLMDELVATGRQAGFPIAGDLPDRMIARTRCMGAYRASTLL
                     DFERGAPLEIESLFLEPLRRARATGIPIPRLEALCAVLEKLEKREMQTGASGDCQCSV
                     APGEGTGPTNR"
     gene            complement(1506..1790)
                     /locus_tag="PKK20_08640"
     CDS             complement(1506..1790)
                     /locus_tag="PKK20_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNU99993.1"
                     /translation="MAKPLTKSQIASTLADKVGITKKQAGEVMATLCELAYKNAKNTF
                     TFPGIGKLVLVNRKARVGRNPATGEEIKIPAKKVVKFRVAKAAKDAILGK"
     gene            complement(1888..2604)
                     /locus_tag="PKK20_08645"
     CDS             complement(1888..2604)
                     /locus_tag="PKK20_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNU99994.1"
                     /translation="MRPPANLDRCRSFIHCQMSPPSAGTPETAKPRKLAVTISRLAGS
                     GAWLVAEHLARFLEAHAPAEGCQWTVFDKELVEKVLEDHNLPRKLAQFMPEDRVSAVQ
                     DMMQEILGLHPPSWTLAHKTVETILKLGELGHVILIGRGANIVTAKLPHVLHVRLVGS
                     IEKRIARVQERLELDRKAAAELIERADRGRQRYLKEHFQARIDDPLDYDLTFNTDRLR
                     CEDVALLIGQAALIRRDTVA"
     gene            3069..5111
                     /locus_tag="PKK20_08650"
     CDS             3069..5111
                     /locus_tag="PKK20_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNU99995.1"
                     /translation="MGQTLTTCTFCGVGCGIYLEAANDRVTGAYPSLSHPTNRGRICV
                     RGWHVHEVASSPERLRSPLLRRGGRLVEATWDEALGYIVGRIKEIRARYGPDALAFFN
                     MPRCSNEESYLLQKLARAVMGTNNVDHGSGPYCNNSINVLRDMLGVSATTNSIGELFR
                     SDAIVVDGVDLGMQLPTIGGIVIRAKLDGAKLIVIDPRRHLLAEHADYFLQVRPGTEA
                     LVYAAMAKVIVDRGLMNQSFVKTHCRDYDAFVAAVGRLDLLGAAEASGVAPELIEGAA
                     LAYGRARAAAWLYSTGVEARNEDSIRSAVNLVLLTGNVGRPGAGVYALTEHNNLQGVC
                     DMGVSPQWLPGYRDVADATARRTFEQAWDTPLPAQPGLGARDMFENGKGSTVKALWLS
                     RYDPVTSAASNNVADVLARMELVVVQHLFPVAAAEQAHVVLPVVAYGEEEVSFTSTDR
                     RIQLARQVLPSPDGPMPVWRQLMFLARSLGAGWAYESSARVMDEISRVVPDYSGVSHE
                     NLGRDYGRQWPCTKDRPLGTPYLFDTGIPGRCFRFRAVAPPAEVALQPAGYPFALIFG
                     HSLYYWHQNVLVKHSETLRRELRVLLLDYPEGFVEISDEDAAALKIRDGGRIRLVAEK
                     GTALTTARVAREVRSGTIYVPFFVREVARDLTGRARSGLGDLDEPVFVRLERVDSQ"
     gene            5108..>6118
                     /locus_tag="PKK20_08655"
     CDS             5108..>6118
                     /locus_tag="PKK20_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNU99996.1"
                     /translation="MRARVIDQADVLKLVDLLRQDRQVIAPFCGRGRDSYFDVVTDVN
                     RDRIRLHVPNPLYPPKRCVLPHIERMMRMRRAAGGLWNFEPDYAETPLALFGVRSCDV
                     AGVRHLDRFYLGREYVDAYYERRRRNLFVVNMVCTDPEVDIDDNCFCVCADTGPAARD
                     AFDLQLMDLGGGEYLVVAGTAAGENLFSAPFFRPATAAHVERRRAILTEVRGRFRSTT
                     SWFAATVRYISRGDVLDETWEEIGNRCLECGACTFICPACTCFTVSDRRIGKDEYERV
                     RMWDSCALKGFTRMAGGFNPRRAVHDRRNRRFFRKLAHYFIQRELSMACVGCGRCAAV
                     CHG"
BASE COUNT         1024 a         1775 c         2156 g         1163 t
ORIGIN      
        1 gtgtcatgtt cataggactg cgttcaggtt ctggttgcgt ttcaacgtcc cgaaccaagc
       61 cgcgggtttg gcacagcccg gcaggaacgc ttccgagcct tataggacaa ccctccctcg
      121 ggcacaacaa ctcttacgag cccgcaactc tgcatcgggt gcatctggag gttgtcggtt
      181 tgtgccgatg tgtgtctttg catcgcgtgg gaacacgcgg cctccaaggt tcggttcatg
      241 gaagcgttga cattcaacgg ttcaatgcgc acgggaccat gaaccggtgc ggagcggcga
      301 cgcctgcctg ccggcaggca gggcaggcag ggcctcgtcg ccccagactc cttcaaatac
      361 attctccggc gggggacgag ccgtcccccc ctccaatggg catcatcggt ttgtaggccc
      421 ggtgccctca ccgggggcga cagaacattg acaatctcca gatgcaccgg tctgcatctc
      481 gcgcttttcg agtttctcga gcaccgcgca cagggcttcg agccgcggga tgggaatgcc
      541 ggtggcccgg gcccggcgaa gtggttcgag gaacaggctc tcgatttcga gcggcgcgcc
      601 gcgctcgaaa tcgagcagag tcgaggcccg ataggcgccc atgcagcgtg tgcgggcgat
      661 catccgatcg gggaggtcgc cggcaatggg aaaaccggcc tggcgtccgg tggcaaccag
      721 ttcgtccatg agttggcgga gcagggcggt ccagcgggga tcgcgaagaa gttcgtcggt
      781 tgccagcgtg gtcactcgat cgagcgtccg cggcaggcgg ccttcgagga cgttctggat
      841 gccggcgatc ccggcgacgc ccaggccgtt gaaagggatg ttccagacca gtttctccca
      901 atgggcctgg gccaggtcgg gcgtcatgtc gcaatggatt ccggctcgaa tgaggcactg
      961 agccacggcc cgggcgccgt cggtgggggc ttgacagtat tggcccagga cgatctggcc
     1021 gtgggcagta tggcggacaa cgccgggcgc gatgcggttg aggcagacga agcagaggcc
     1081 gcccaggaca tggtcggggc cgaagagggc ggccagagcc tcttcattgc ccaggccgtt
     1141 ttggagggta agaatccagg attggggacc gaccagcggg ggcagcaggc tggggaaggc
     1201 gtggttggca gtggccttga gcgcgatgat gacgagatcg caaacgccga tctcggccgg
     1261 ttgccgggcc gggcacggga aagcccggaa ctcgccgtcg gggctgatga tgcgcacgcc
     1321 ttcctggcgg acgacctcat agtcggatcg cagcagaaag aagaccggga gaccggcgcg
     1381 gcagagtttg gcgccgtaga agctgccgac tgcgccacag ccgacgaccg cgactttcat
     1441 gatgcggggt gaaacaaaaa caggcacccc gagtcgatca gggtgcctgc gcggggagag
     1501 aacggttact tgccgaggat tgcgtccttg gcggccttcg cgacgcggaa tttcacgacc
     1561 ttcttggccg ggattttgat ttcttcgcct gttgcggggt tgcgtccgac acgcgccttg
     1621 cggttcacaa gcaccaactt gccgatgccc gggaaggtga aagtgttttt agcgttctta
     1681 tatgccaact cacacagtgt ggccatcacc tcgcccgcct gcttcttggt gatgccgact
     1741 ttgtcggcga gggtggaggc aatttgggac ttcgtcaatg gtttagccat aatctatcta
     1801 tcgggtttaa tggttaggta aattcaccgg tcgacgcggg tggttgtagc aagcttcagc
     1861 agtggcgcaa gtagaaaatc caacgtttca ggccactgtg tcacgccgga tcagggccgc
     1921 ctgcccgatg aggagcgcaa catcctcgca ccggaggcga tcggtgttga atgtgagatc
     1981 gtagtccaag ggatcatcga ttctggcctg gaaatgctcc ttgagatagc gctgccggcc
     2041 ccgatccgct ctctcgatca gttcggcggc cgccttgcga tcgagttcga gccgctcctg
     2101 gacgcgggcg atccgtttct cgatcgagcc caccaggcgg acgtgaagga catgcggcag
     2161 cttggcggtg acaatgttgg cgccgcgccc gatcagaatc acgtggccga gttccccaag
     2221 tttcagaatg gtctcgacgg ttttgtgggc gagcgtccac gagggcggat gcaggccgag
     2281 gatttcctgc atcatgtcct ggactgccga gacgcggtct tcgggcatga actgcgcgag
     2341 cttgcgcggg agattgtgat cctcgagcac cttctcgacc agttccttgt cgaacactgt
     2401 ccattggcat ccctccgcgg gcgcgtgcgc ctcgaggaat cgcgcgaggt gttcggccac
     2461 caaccaggca ccggacccgg cgagtcgcga aatggtcaca gcaagcttgc ggggcttggc
     2521 ggtctccgga gtgcccgccg agggcgggga catctggcag tgaatgaaac tgcggcatcg
     2581 gtccaggttg gcgggcggtc tcataacggg ttcctttcag ccaccgttcg agcatatcag
     2641 gttccgccgg tttgcccaag cgtttttctg gatcgggcag gcggtgcatt gggttcttgt
     2701 cggcgggaat cgagtttgcg ccgcgtgggc ctgtttgccg cgcctgcctg tgtcgtgccc
     2761 gacacggcag acaggccgct gcgctttgcg gcgcaggcag ggcacgcggc ctacgtgcga
     2821 ttacgattac gacaagccgg gaagactgaa agagctgaga tgggtcgccc aatgcgatcg
     2881 ggtgcagatc gggtgcaaat cggagttggc atttccagcg ggtcactcta ggttccccgc
     2941 gcgcatgccg catccgcgcg tgcccgctcg gcgcaattgg aacgagagtc gaggccggga
     3001 tgcgcacgga cgaacgtcct gcggtccccg cgatgggggc tggcgcgggc cggcgaagcg
     3061 aaaccgacat gggacaaacg ctgacgacat gcaccttttg cggtgtcggg tgcgggattt
     3121 acctggaagc ggcgaatgac cgggtcaccg gggcctatcc cagcctctcg catcccacga
     3181 accggggccg aatctgcgtg cgcggctggc acgtgcatga agtggcgagt tcgcccgagc
     3241 ggctgcgatc gccgctgctg cgccgcggtg ggcggttggt ggaggcgacg tgggacgagg
     3301 ccctgggcta catcgtgggt cggatcaagg agattcgggc gcgttacggg ccggacgcgc
     3361 ttgcgttctt caacatgccg cgctgttcga acgaggagag ttatctgctg cagaaactcg
     3421 cgcgcgcggt gatgggcacc aacaacgtcg atcacgggtc cggcccatac tgcaacaaca
     3481 gcatcaacgt cctgcgcgac atgctcggcg tctcggcaac caccaactcg atcggcgagc
     3541 tgttccgcag cgatgcgatc gtggtcgacg gggtagacct gggcatgcaa ttgcccacca
     3601 ttggcggcat cgtcatccgc gccaaactcg acggggccaa gctgatcgtg atcgatccgc
     3661 ggcgccattt gttggccgag catgcggact attttctaca ggtgcgtccg gggacggagg
     3721 cgttggtcta cgcggcgatg gccaaggtga ttgtagaccg cgggctgatg aaccagtcct
     3781 ttgtcaagac gcactgtaga gattacgacg ccttcgtcgc ggcggtgggc cgcctcgatc
     3841 tgctgggggc ggccgaggct tccggtgtcg cgcctgaact gatcgagggg gcggctctgg
     3901 cttacgggcg ggcccgcgct gccgcgtggc tctactcgac cggcgtcgag gcgcgcaacg
     3961 aggacagcat ccgatcggcg gtcaacctgg tcctgctgac cgggaatgtc gggcggccgg
     4021 gggccggggt ctacgcgttg accgagcaca acaatttgca gggggtgtgc gacatggggg
     4081 tctcgcccca atggctgccg ggctaccggg atgtggccga cgccacggcg cggcggacgt
     4141 tcgagcaggc ttgggacacg ccgctgccgg cccagcccgg tctgggggcg cgcgatatgt
     4201 tcgagaacgg gaagggctcg acggtcaagg cgctctggct aagccggtat gatccggtga
     4261 catcggcggc atcaaacaat gtggcggacg tcctggcgcg aatggagttg gtggtcgtgc
     4321 agcatctctt cccggttgcg gcggcggagc aggctcatgt cgtcctgccg gtggtggcct
     4381 acggcgagga ggaagtctcc ttcaccagca ccgatcgccg catccagctc gctcgccagg
     4441 tgctgccctc tcccgacggg cccatgccgg tctggcggca gttgatgttc ctggcgcgga
     4501 gtttgggcgc cggctgggcg tacgagtcga gcgcgcgtgt catggacgag atcagcaggg
     4561 tggtgcccga ctacagcgga gtgagccatg agaatctcgg gcgcgactac gggcgccagt
     4621 ggccgtgcac caaggaccgt ccgctcggca cgccttatct tttcgacacg ggcattcccg
     4681 gccggtgctt ccggttccgc gcggtcgcgc cgccagccga ggtggccctg cagccggcgg
     4741 gttacccctt tgcattgatt ttcgggcatt cgctgtatta ctggcatcaa aacgtgctcg
     4801 tgaagcacag cgagacgttg cgtcgggaac tgcgggtgct gctgctcgat tatcccgaag
     4861 gattcgtgga aatcagcgat gaagacgccg ccgctctcaa aatacgggat ggcggtcgca
     4921 tccgcctggt ggccgagaaa gggacggcgc tgacgaccgc ccgggtggcg cgtgaagtgc
     4981 ggagcgggac gatttacgtc cccttcttcg tgcgagaggt cgcgcgcgat ctgacaggac
     5041 gggcgcgcag cggtttgggc gacctcgacg agcctgtctt tgtgcggttg gaacgggtgg
     5101 attcgcaatg agagcgcgtg tgatcgatca ggcggacgtc ttgaagctgg tggacctgct
     5161 gcgtcaggac cgccaggtga tcgccccgtt ctgcgggcgg ggccgggaca gctatttcga
     5221 tgttgtgacg gatgtcaatc gggatcggat tcgcctccac gtgcccaatc cgctctatcc
     5281 gcccaagcgc tgtgtcctgc cgcacatcga gcggatgatg cggatgcggc gggcggcggg
     5341 cggcctttgg aatttcgagc cggactatgc cgagacgcct ctggcgcttt ttggcgtccg
     5401 ttcctgcgac gtggcgggcg ttcggcatct ggaccgcttc tacctcgggc gggagtatgt
     5461 ggatgcgtac tacgagcggc ggcggcggaa tctgttcgtc gtgaacatgg tttgcacgga
     5521 tcctgaagtc gacatcgacg acaactgttt ctgtgtctgc gcggacaccg ggcccgcggc
     5581 gcgcgatgcc ttcgatctgc aactgatgga tctgggtggc ggagagtacc tcgtggtggc
     5641 cgggaccgcg gccggcgaga atctcttcag cgctccgttc ttccgcccgg ccaccgcggc
     5701 gcatgtcgag cggcggcggg cgattctgac ggaggtccgc ggacggttcc ggagcacgac
     5761 cagttggttt gcggcgacgg tgcggtacat ctcgcgcggg gacgtgctcg acgagacgtg
     5821 ggaggagatt gggaaccgct gtctcgagtg cggggcgtgc acgtttatct gccccgcctg
     5881 cacctgtttc accgtaagcg accgacgcat cgggaaagac gaatacgaac gggttcggat
     5941 gtgggattca tgcgccctga agggcttcac gcggatggcg ggcggattca atccgcggcg
     6001 ggcggtgcat gaccgacgca accgccggtt tttccggaag ctggcgcatt atttcatcca
     6061 gcgggagctg tcgatggcct gtgtgggttg cgggcgctgc gcggcggtgt gtcatggc
//
LOCUS       DAOQRO010000211         6100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13007_length_6100_cov_4.507031, whole genome shotgun sequence.
ACCESSION   DAOQRO010000211 DAOQRO010000000
VERSION     DAOQRO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13007_length_6100_cov_4.507031"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..203)
                     /locus_tag="PKK20_08660"
     tRNA            complement(129..203)
                     /locus_tag="PKK20_08660"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Cys,seq:gca)
     gene            complement(263..1339)
                     /gene="prfA"
                     /locus_tag="PKK20_08665"
     CDS             complement(263..1339)
                     /gene="prfA"
                     /locus_tag="PKK20_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNU99997.1"
                     /translation="MDLRPYIAQFNQRLAEIESALSDPKVFTNTQRTQELAREHSRLK
                     NLQDQGTAYLNALAELDANRALLAAETDPELVRMAQDEIARLEPEAQRMAQKVQAGII
                     PPDPSDSRNTIVEIRAGAGGAESALFAADLYRMFSRYAEIQGWKIETIDASGSDLGGL
                     REVVFSVTGTDVFKRLKYESGVHRVQRVPATEAQGRIHTSTATVAVLPEAEEVDIELN
                     DADLEFTVCRASGPGGQGVNTTDSAVQLVHKPTGTIVRCSDERSQQKNKAKALKVLRS
                     RLLDQRVAAENAKYAAQRRAQVGTGERNEKIRTYNFSQNRVTDHRIELSVYNLSAVMD
                     GDLDCLIEPLIADDVQRRLAALKT"
     gene            complement(1523..1777)
                     /gene="rpmE"
                     /locus_tag="PKK20_08670"
     CDS             complement(1523..1777)
                     /gene="rpmE"
                     /locus_tag="PKK20_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNU99998.1"
                     /translation="MRDGIHPKYQEAEVRCACGNSFKTRSTRPVIVLGICNVCHPFYT
                     GQQKFVDTAGRVDKFQQRMAKTKAAQDAIAAAAASKKRKR"
     gene            1879..1953
                     /locus_tag="PKK20_08675"
     tRNA            1879..1953
                     /locus_tag="PKK20_08675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1911..1913,aa:Asn,seq:gtt)
     gene            complement(2456..5986)
                     /locus_tag="PKK20_08680"
     CDS             complement(2456..5986)
                     /locus_tag="PKK20_08680"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNU99999.1"
                     /translation="MRHTTTSLLSPLLGTFIGVMSLAQDLAKDFANPPDSARPGAYWY
                     FMDGNQDREAMTADLECMHQAGLRKALFLEVNIGVPRGPVDFMSPIWQENFAHAVRTA
                     DRLGMEIILGTGPGWSGAGGPWIDPPRSMQHLCASSVRVVGPAPCTAILPVPPPRAPS
                     QFAGLSPDLAAQRDAWHADVAVLAFPTPAATEPPENLAVKTLYETQPYSIWKHVPRFI
                     PAPASHSEPPAGAIIDLNQVLDLTTRLRTDGSLDWTVPPGDWTVMRFAARSTGATTRP
                     APTPGHGFETDKFDAAAFAFHFDQFHKKLLEQVGPRRPGRGWTALHLDSWEMSSQNWS
                     ARFRAEFQRRRGYDPQPFFPAYTGLIVGSRENTERFLWDLRKTAQELVIENHAHIIRR
                     HAHQNGFYYSNQPYDMNPAGDIELGAVADIPSCEFWIAEVDTVYSCIEAASIAHTMGR
                     PVVRAEAFTSPPRHGYPDNPNDLKNQTDWAFAMGINDLIFHTFQHQPLGLDAPKPGIA
                     MGPHGIHWHRHQTFWPMVRPYHDYIARCGQMLRQGASVADVLYLVLEGAPHIFLPPDD
                     AFEGQGLLREKKAYGFDAVSPGILMSRAKADNGRIVFPEGTTYRLLVLPIVETMTPEL
                     LAKLEDLVHKGATILGNPPVKSPSLSQYPQCDARVRALARKMWGKLDVPDTAVERPYG
                     KGRIVWGGPATAPERPSHPSLMHQRSHWIWTPQDDPATHAPAGVTRCFQKTFQLESGR
                     TPRTARLHATADNGFEVWLNGVAVASGNNFNEIQQVDIRPALKPGTNLLAVAASNAGS
                     EPNPAGLIALVHIEFENGDDFFVGTDSDWRVADNPEEAWQGEPKTGPHWRAARVLGPA
                     SMAPWTNVIPKSGSASLYPSYEATVALLKTWGVPPAFDTTGPLRYHQRRTPTRDIFFV
                     GNRTAEPVAIECAFRTDGAAPQLWDPITGARRSLPRYTSRDGIVSVPLQFAACESWFI
                     VFDRRANGTAANVQGENFSAETILATLDGLYEVTFDPAWGGPAKPVAFDTLLRWDKHA
                     DEGIRYYSGQAVYRKTVNRPHDLSDAERVVLDLGIVHKLAGVKLNGKDLGIALTSPFR
                     VDFPADLLRPTGNLLEITIVNTWVNRLIGDQQPAHKAVRQLEWKSGLLGGVPHAAGRY
                     TFTTAAGDYTASSPLEPSGLLGPVTLRAVR"
BASE COUNT         1034 a         1661 c         2201 g         1204 t
ORIGIN      
        1 taccagtggt tgccgagtac tgccggggtt ttcgatgcgt attggcccgg ttttgatttc
       61 actggcactt tcactggcac ttgccgtctc tggaatctgc aactctttga ggaacaaacc
      121 gttggagttg gaggcgaggg tcggaatcga accgacgcat aagggttttg cagacccctg
      181 ccttaccact tggctacccc gccgccgtga cagaccgaag gtaaaacggc tgcgcgcggg
      241 cgcaagtctt aaatgcccag gttcaagtct tgagagcggc gagccggcgc tgaacgtcat
      301 cggcaatcag aggctcgatc agacagtcga ggtcgccgtc catgacggca gaaaggttgt
      361 agacactcaa ctcgatgcgg tggtcggtga cgcggttctg agagaagttg taggtgcgaa
      421 ttttctcgtt gcgttcgccg gtgccgacct gggcgcggcg ttgggcggcg tacttggcgt
      481 tttcggcggc gacgcgttgg tcgaggagcc tcgatcggag aaccttgagg gctttggcct
      541 tgttcttctg ctgggagcgt tcgtcggagc aacgaacgat cgtgccggtg ggcttgtgca
      601 cgagttgcac cgcggaatcc gtggtgttaa cgccctgtcc gcccgggccg gaggcgcggc
      661 agacggtgaa ttcgagatcg gcgtcgttga gttcgatgtc gacttcctcg gcttcaggca
      721 ggacggcgac ggtggcggtg ctggtatgga tgcgtccttg ggcttcggtg gcagggacgc
      781 gctggacgcg atggacgccg ctctcgtatt tgaggcgttt gaagacgtcc gtgcctgtga
      841 cgctgaagac gacctccctc aaaccgccaa ggtcggagcc gctggcgtcg atggtctcga
      901 ttttccagcc ttggatctcg gcgtatcggc tgaacatgcg atagagatcg gcggcgaaga
      961 gcgcggactc ggcccctccg gccccggcac ggatctcgac gatggtattg cgagagtcgg
     1021 aggggtcggg ggggatgatg cccgcctgga ctttttgcgc catgcgctgg gcctcgggtt
     1081 cgaggcgggc gatttcgtcc tgggccatcc tgaccagttc cgggtcggtc tccgcggcga
     1141 ggagggcgcg gttggcgtcg agttcggcaa gggcattgag gtaagccgtc ccctggtcct
     1201 ggaggttttt gagccgggaa tgttcgcgcg ccagttcctg ggtacgctgg gtgttggtga
     1261 agaccttggg atcgctcaag gcgctctcga tctcggcaag gcgctggtta aactgcgcga
     1321 tgtacgggcg taagtccatg gagagcatcg acattcaacg acttaatgca catggggacc
     1381 gtggaccagg aacgttcaac gtccaacgtt caactagcaa cggtcaacca tgaaccatca
     1441 accatcaacc gggagagaca ggctcagaaa aagagtccgc ccgttcggcg ggaagccgga
     1501 cgggcggagg ctcggcaaaa ggctaacgtt ttcttttctt ggacgcagcg gcggcggcga
     1561 tggcgtcctg ggctgccttg gtcttggcca tgcgctgttg gaacttgtcc acgcgtccgg
     1621 cggtgtcgac gaacttctgc tggcccgtgt agaaggggtg gcagacattg cagatgccga
     1681 ggacgatgac cggtcgggtc gaccgggtct tgaaactatt gccgcaagcg catcgcacct
     1741 cggcttcctg atacttcgga tgaatcccgt cacgcatacg ttaaagactg agaaccatac
     1801 cggtcgccgg gcggctggca agcgcgaaaa tcggttgacg cgaggcgggt gcgtcgatat
     1861 tttatgcgct ctctgagttc cagagtagct caatggtaga gcagccggct gttaaccggc
     1921 gggttagagg ttcgagccct ctctctggag ccaattcctc cggcctttcg gcgccagcaa
     1981 caccctggac tgcaagagtc tcacggcccg ataaccggag ggggtgatcc ggcgggaatt
     2041 cgaagttgat cgggcgatcc ttctgacgct gatgcaccgg ttgggaaagt ccacggtcgg
     2101 cgggggaggt cacttgggtt ggcagagcga ggactcgctc ggtgaccgcg acgcagccgg
     2161 cgagttcggg catggtgttt gtgcagtgcc accaggtctc gagcgcttgg gacgggtgca
     2221 tgaccagctt gcccagcgcg ttggtcggat agtgttgctc gaagaaggtc agaatctcgc
     2281 gtgcgaaggg cagcgcggtg ttctgcagga agtcgcggtc gagcgtgtgg tcgaagtaat
     2341 cgagcagtcc tcttgcgtag tctcagtgcc ctgtgcgcca taggggtgtg aaatatgcgg
     2401 ggtagcgctg cgcttttgtg ttggtctcgg cacatcattt ccgcatcccg atgcgtcatc
     2461 gaacggcccg cagcgttacc ggaccgagca ggccggatgg ttcaagaggc gagctggccg
     2521 tgtagtcgcc tgcggcggtt gtgaaggtgt agcggccggc cgcatgcggg acgccgccaa
     2581 gcaagccgga cttccactcg agttgtcgga ccgccttgtg cgcgggttgt tggtctccga
     2641 tcaaccggtt gacccaggtg ttgacgatcg tgatttcgag gagattgccc gtgggacgca
     2701 gcaggtcggc agggaagtcg acgcggaacg gggaagtcaa ggcaataccc aggtctttcc
     2761 cgttgagctt cacgcccgcc agcttgtgga caatccccag gtccagaacg acgcgctccg
     2821 cgtccgacag gtcgtgcggg cggttgacgg tcttgcggta gacggcctgg cccgagtagt
     2881 agcggatgcc ttcgtctgcg tgtttgtccc atcggagcag agtgtcgaag gcaaccggct
     2941 tggcagggcc gccccaggcg ggatcaaacg tcacttcata gagtccatcc agtgtcgcca
     3001 ggatcgtttc ggccgagaaa ttctcgccct ggacattcgc cgccgtgccg tttgcccggc
     3061 gatcgaacac aatgaaccag ctctcgcacg cggcgaactg cagtggcaca gacacaatgc
     3121 cgtcgcggct ggtgtagcgg ggcagcgagc ggcgggcgcc cgtgatcgga tcccagagtt
     3181 gaggggcggc gccgtctgta cggaacgcgc actcgattgc gaccggttcg gcagtgcgat
     3241 tgcccacgaa gaagatgtcg cgggtcgggg tgcggcgctg gtgatagcgc agcgggccgg
     3301 tggtgtcgaa ggccggcggg acaccccatg ttttcagcaa cgcgacagtg gcttcgtagc
     3361 tcgggtaaag ggaggctgat ccggacttcg ggatgacatt ggtccaaggt gccatcgagg
     3421 cgggccccag gacccgtgcg gcgcgccagt gcggacccgt cttaggttcg ccctgccagg
     3481 cctcttcggg gttgtccgcg acacgccagt cggagtcggt gcccacgaag aaatcgtctc
     3541 cgttttcgaa ctcgatgtga acgagtgcga tcaagccggc gggattgggt tcggacccgg
     3601 cattcgaggc ggccacggcg agcaggttgg ttcccggttt gagggcgggg cggatatcga
     3661 cctgttggat ttcgttgaag ttattgcctg aggcgacggc gacgccattg agccagactt
     3721 cgaatccgtt gtcggctgtg gcgtggaggc gcgcggtccg cggagtgcgt ccgctctcga
     3781 gttggaatgt cttttggaag cagcgcgtca cgccggctgg ggcgtgtgtg gcgggatcgt
     3841 cctggggcgt ccagatccag tggctgcgct ggtgcatgag gctgggatgg ctgggccgtt
     3901 ccggggcggt tgccgggccg ccccatacga tccggccttt cccatagggg cgctcgacgg
     3961 ctgtgtcggg gacatcgagt ttgccccaca tcttccgggc cagggcgcgg acgcgagcat
     4021 cgcactgggg atactgggac aggctggggg acttgactgg gggattgccg aggatcgtgg
     4081 cgcccttatg aaccaggtct tcgagtttgg cgagcagttc cggagtcatg gtctcgacga
     4141 tcggcaggac caagaggcga taggtcgtgc cttccgggaa gacgatgcgt ccgttgtccg
     4201 cctttgcgcg ggacatgagg atgccggggc tgacggcatc gaatccgtag gccttctttt
     4261 cgcggagcaa tccctgaccc tcgaaggcgt cgtctggcgg caggaagatg tgcggggcgc
     4321 cctcgagcac caggtagagg acatcggcca ccgaggcacc ctgacgcagc atttggccgc
     4381 agcgggcgat gtaatcgtga tatgggcgga ccatgggcca gaaggtttgg tggcggtgcc
     4441 agtggatgcc gtgcggtccc atggcgatgc ctggtttggg ggcgtcgaga cccagcggct
     4501 gatgttggaa cgtgtgaaag atgaggtcat tgattcccat ggcgaacgcc cagtcggtct
     4561 ggtttttgag atcgttgggg ttatccgggt aaccgtggcg gggcggcgat gtgaaggctt
     4621 cggcgcggac gaccgggcgg cccatcgtat gggcgatcga ggccgcctcg atgcaactat
     4681 agaccgtgtc cacctcggcg atccaaaatt cgcaggacgg aatgtcggcg accgcgccca
     4741 actcgatgtc gccggcgggg ttcatgtcgt agggctgatt cgagtagtag aagccgttct
     4801 ggtgggcgtg gcggcggatg atgtgcgcgt ggttttcgat caccagctcc tgggctgtct
     4861 tgcgcaaatc ccagagaaaa cgctcggtgt tctcgcgcga gccgacgatc agccccgtgt
     4921 aggcggggaa gaacggttgt ggatcgtaac cgcgccggcg ttggaactcg gcgcgaaacc
     4981 gggctgacca gttctgggaa ctcatctccc aactgtcgag gtgcagagct gtccagccgc
     5041 gtccgggacg ccgtgggccg acctgttcga gcagcttctt gtggaactgg tcaaagtgga
     5101 aagcgaatgc cgctgcgtcg aatttgtcgg tctcgaagcc gtgtccgggc gtgggggctg
     5161 ggcgggtggt tgccccggtg cttcgggcgg cgaatcgcat cactgtccaa tcgcctggcg
     5221 ggacagtcca gtcgagcgag ccgtcggtgc gcaagcgcgt tgtcagatcg agaacctggt
     5281 tcaggtcaat gatggcgcct gcgggcggtt cgctgtggct ggccggggcc gggatgaagc
     5341 gcggcacgtg tttccaaatc gagtagggct gcgtctcata gagcgtcttg acggccagat
     5401 tctccggcgg ttcggtcgca gccggcgtcg gaaacgccag gacagccaca tccgcgtgcc
     5461 aagcgtcgcg ctgggcggct aggtcgggcg acaggccggc gaattgggat ggggcgcgcg
     5521 ggggaggaac cgggagaatg gcggtgcacg gggcggggcc gacgacccgg accgagctgg
     5581 cgcagaggtg ctgcatcgag cggggtggat cgatccaggg accgccggca ccgctccagc
     5641 cggggccggt gccgaggatg atctccatgc cgagccggtc tgccgtgcgg acggcgtggg
     5701 cgaaattctc ctgccagatc gggctcatga aatcgaccgg gccgcggggc acgccgatgt
     5761 tgacttcgag gaatagggcc ttgcgcaagc cggcctggtg catgcattcc aggtcggccg
     5821 tcatcgcctc gcggtcctgg ttgccgtcca tgaagtacca gtaagcgccc ggtcgggccg
     5881 aatcgggcgg gttggcgaag tcctttgcca aatcctgcgc gaggctcatc actccgatga
     5941 acgtgccgag gagcggcgaa aggagggagg ttgtggtgtg acgcattctc cgccgcggag
     6001 cgtgcggtac gatcgagagg aagggcgcgg acatggttcg atttgttgga aaggtagtga
     6061 gcatgcgcgt acggtgccag tcgaatcggc gtcgttgtcg
//
LOCUS       DAOQRO010000212         6096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13029_length_6096_cov_4.856481, whole genome shotgun sequence.
ACCESSION   DAOQRO010000212 DAOQRO010000000
VERSION     DAOQRO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13029_length_6096_cov_4.856481"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..258
                     /locus_tag="PKK20_08685"
     tRNA            182..258
                     /locus_tag="PKK20_08685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Arg,seq:tct)
     gene            274..1161
                     /locus_tag="PKK20_08690"
     CDS             274..1161
                     /locus_tag="PKK20_08690"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNV00001.1"
                     /translation="MLTLEHLAELVEGHRAATTARVREFKVRGKPLFFNTRPAIMGVI
                     NLSAESWYRESVCLDPARAIHRGQVMHAQGADLVDVGAESTLSHAARVDDALQNSRLL
                     PVVRALNAGGIPVSVETYQPDVARACLDAGACVLNLTGTARTEEIYQMVAAHDAAVII
                     CYVQGPNVREVEDFDFSRNPVDQMYEFFARQTEIARRNGVEKIFIDPGLGFYYRNLQD
                     SALRVQHQMRVFLNTFRLRALGFPTCHALPHAFEYFAEEVRSAEAFFAVLALLGRTDL
                     LRTHEVPRVKAVLETMRAY"
     gene            1173..3221
                     /gene="uvrB"
                     /locus_tag="PKK20_08695"
     CDS             1173..3221
                     /gene="uvrB"
                     /locus_tag="PKK20_08695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNV00002.1"
                     /translation="MFELVSPFAPAGDQPQAIAHLVAALRAGVPHQTLVGVTGSGKTF
                     TIANVIQEVNRPTLVISHNKTLAAQLYAEFKSFFPRNAVEYFVSYYDYYQPEAYIPRT
                     DTFIEKDSSRNEEIERLRLSTMSSLFARRDVIVVASVSCIYGIGSKDDYEAMVIPMRV
                     GQEIARDKLLGRLVEILYTRNDVAFERGQFRVRGDTVEVCPAYAEDALRIEFFGDQIE
                     RLTRFDPLSGRKIAELEAVTIYPAGQFVTPMESIRRAILSIREELTDRIAWFEARGKL
                     LEAQRIKMRTEYDLEMMEEMGFCSGIENYSRHIAGRPAGSRPCTLLDFLPEDFLMVID
                     ESHATIPQIGGMHAGDQSRKRVLVEHGFRLPSALDNRPLSFSEFQSLQRQTIYVSATP
                     GTRELTWSQGRLVELIVRPTGLLDPKITLKPLKGQIDDLIEEARQRVDRKERVLVTTL
                     TKRTAEQLTDYLRDIGIQVRYLHSEIDAIERVEILRALRKGEFDVLVGINLLREGLDL
                     PEVSLVAILDADKEGYLRSTTSLIQTAGRAARHLNGEVILYADVRTESIGKFLEVSEY
                     RRGKQEAYNREHNITPRSVSRAVEESLSSRREKADQAMALLREGGGDFDVTETIRELE
                     TEMLEAANNLEFEKAALLRDQIRELKRQYEAAPGKAVETARRAGRVSYRRSKNRGNRR
                     "
     gene            complement(3296..3442)
                     /locus_tag="PKK20_08700"
                     /pseudo
     CDS             complement(3296..3442)
                     /locus_tag="PKK20_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(3741..4721)
                     /locus_tag="PKK20_08705"
     CDS             complement(3741..4721)
                     /locus_tag="PKK20_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV00003.1"
                     /translation="MTTQSKTAWVTGAAGFIGSHLVDRLLDRGWRVLGMDNLRLGRTS
                     NLRKAFEHTEFSFFEADINDYETNLRRLRDFHARRPIDMVWHLAANSDIRAGALDPQV
                     DFEHTFLTTFTVLRLMRALGLRRLAFSSSSAIYGMRPGLLDEDTGPCFPVSNYGAMKL
                     ASEGAVSAGLETHLERAWILRFPNVVGSRGTHGVIFDLLQKLRADPSVLEVLGDGRQE
                     KPYLHVSELVDAMLFVVDRACERLNCYNIGTDGTASIVSWIAQTVIRAAAPGATIRYT
                     GGAQGWPGDVPRFQYSIERLRRLGWAPALTSDQAIERAVAELLAERCEQG"
     gene            complement(5210..5839)
                     /locus_tag="PKK20_08710"
     CDS             complement(5210..5839)
                     /locus_tag="PKK20_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV00004.1"
                     /translation="MKTETTLTAAAAVLTGAASAAAADAAAVNDLIAKIKSADDAVRG
                     PAWQGAGPVGAPAVAPLAEVMSDPHFEIARSAKRAIEKIARHAGRPGANPERKAVEAE
                     LIKLLSHSNANVRRHAVWMLSEVGDAAAVGPMAALLADAEVREDARCALTRVPGSQAT
                     DALRGAMDGAPEEFKYALAESLRARGETVAGYPSKKLVPVKQTSVKAEA"
     gene            complement(5877..>6096)
                     /locus_tag="PKK20_08715"
     CDS             complement(5877..>6096)
                     /locus_tag="PKK20_08715"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00005.1"
                     /translation="DHHEDWFEGIKTGRKTIMNIEGGVATAFLCVLGNLSLIVGRKLA
                     WDPMRQEIVGDEAARRLMSRPQRFPYAL"
BASE COUNT         1121 a         1968 c         1949 g         1058 t
ORIGIN      
        1 gtcgttcctc ggtcggtcga gtatctcagg ggatacactc cctccctcgg gcctctcatc
       61 tggcccggtg gcgctcccgc caaaaccgga attaattttt gcacagaccc taagcgggct
      121 tgccccgcca cctcgacccg cgtaacatgc ccctgaacgc ggactggatg ttgatggctc
      181 tgcccccgta gctcagttgg atagagcatc tgccttctaa gcagagggtc gcgcgttcga
      241 gtcgcgccgg gggtgccact cttcagttga gttatgttga ctctcgagca tctggccgaa
      301 ctggtagaag ggcatcgggc ggcaaccacg gcccgggtcc gggagttcaa agtgcgggga
      361 aaacccctgt tcttcaacac gcgcccggcg atcatgggag tcatcaattt gtcggccgaa
      421 tcctggtatc gcgaaagcgt ctgcctcgac cccgcgcgcg cgattcatcg tggccaggtg
      481 atgcacgccc agggggccga cctggtggat gtgggagcgg aatcgaccct gtcacacgcc
      541 gcgcgtgtgg acgacgcgct gcagaacagc cgattgctgc ccgtggtgag agccctgaac
      601 gccggcggca tccccgtctc ggtcgagacc taccaacccg atgtggcgcg ggcctgtctg
      661 gacgccggcg cgtgcgtgct gaacctgacc gggaccgcgc ggacggagga gatataccag
      721 atggttgcgg ctcacgacgc ggctgtgatc atctgctacg tgcaggggcc caacgtgcgc
      781 gaggtggagg acttcgattt cagccggaat ccggtggatc agatgtacga gttcttcgcc
      841 cggcagaccg agattgcgag gcgcaacggc gtcgagaaaa tcttcatcga tccgggcctc
      901 gggttctact accgcaacct ccaggacagc gcgctgcggg tgcaacatca gatgcgcgtc
      961 ttcctcaaca cctttcggtt gcgcgcgctg ggcttcccga cctgccacgc cctgccgcac
     1021 gccttcgagt acttcgccga ggaagtgcgg tcggccgagg cgttcttcgc ggtcctggcg
     1081 ctgctcggac gcaccgatct gctgcggacg cacgaggtgc cgcgggtcaa ggcggtgctc
     1141 gagaccatgc gggcgtactg attctccggc ctgtgttcga actcgtctcc cccttcgccc
     1201 ctgccggcga ccagccgcag gccatcgcgc acctggtggc ggccctgcgc gcgggcgtgc
     1261 cgcaccagac gctcgtgggc gtgacgggct cgggaaagac cttcaccatc gcgaacgtca
     1321 tccaggaggt gaatcgcccg accctggtca tctcccacaa caagaccctc gcggcgcagc
     1381 tctacgccga attcaagagc ttctttccgc gaaacgcagt cgagtacttc gtcagctatt
     1441 acgattatta ccagcccgag gcgtatatcc cgcgcaccga cacgttcatc gagaaggact
     1501 cgagccggaa cgaggaaatc gagcggctgc ggctctcgac gatgagttcg ctgttcgctc
     1561 ggcgcgacgt gatcgttgtg gccagcgtgt cgtgcatcta cgggatcggg agcaaggacg
     1621 actacgaggc gatggtgatc ccgatgcgcg tcggacagga gatcgcgcgc gacaagctcc
     1681 tcgggcgcct ggtcgagatt ctctacaccc gcaacgacgt cgctttcgag cgcggtcagt
     1741 tccgcgtccg aggcgacacc gtcgaggtgt gtcccgccta cgcggaggac gcgctgcgca
     1801 tcgagttctt cggcgatcag atcgagcggt tgacccgatt cgatccgctg agcgggcgca
     1861 agatcgcgga actcgaggcc gtcaccatct acccggcggg gcaattcgtc acgcccatgg
     1921 aaagcatacg ccgggcgatc ctgtccatcc gcgaggagct gaccgaccgg atcgcgtggt
     1981 tcgaggcgcg cggaaagctt ctcgaggccc agcggatcaa gatgcgcacg gagtacgatc
     2041 tcgagatgat ggaggagatg ggattctgct cgggcatcga gaattactcg cgccacatcg
     2101 ccgggcgccc cgcaggatcg cgtccgtgca cgctgctcga tttcttgcct gaggatttct
     2161 tgatggtgat tgacgaatcg cacgccacga ttccccagat cggcgggatg cacgcggggg
     2221 accagtcccg caagcgcgtg ctggtcgagc atggctttcg cctcccgagc gccctggaca
     2281 accgtccgct gagtttcagc gagttccagt cgttgcaacg gcagacgatt tatgtgagcg
     2341 cgacgcccgg aacgcgggag ttgacatggt cgcaggggcg cctggtcgag ctcattgtcc
     2401 ggcccaccgg gcttctcgac ccgaagatca cgctcaagcc gctcaaagga cagatcgacg
     2461 acctgatcga ggaagcccgc cagcgggtgg accgcaagga acgtgtgctt gtcaccaccc
     2521 tgaccaaacg caccgcggaa caattgaccg attacctgcg ggacatcggg attcaggtgc
     2581 gatacctgca cagcgagatc gacgcgatcg agcgggtcga gattctgcgg gcgctgcgga
     2641 agggcgagtt cgacgtgctt gtgggcatca atctcctgcg cgaggggctc gacttgcccg
     2701 aggtctcgct ggtggcgatc ctcgatgccg acaaggaggg ctacctgcgc tcgaccacca
     2761 gcctgatcca gaccgccggc cgcgccgccc gtcatctgaa cggcgaggtg attctctacg
     2821 cggacgtccg aacggagagc atcgggaagt tcctcgaagt gtccgagtat cgccgcggga
     2881 agcaggaggc ctacaaccgg gaacacaaca tcacgccccg cagcgtcagc cgggccgtcg
     2941 aggaaagtct ctcgtcacgc agggaaaagg ccgaccaggc catggcgctg ttgcgcgaag
     3001 ggggcggcga tttcgacgtg acggagacga ttcgcgaact cgagaccgag atgctcgaag
     3061 cggcaaacaa cctcgagttc gagaaggccg cgctcctgcg ggaccagatt cgcgaactga
     3121 agcgccaata cgaagccgct ccgggcaaag cggtcgagac ggcccgaagg gcaggccgag
     3181 tgagctatcg ccggtccaag aaccgtggca accggcgttg accggatcgg atcgccgttc
     3241 acggccgccc ggcgtgctcg acaatgaagt ccacgatcgg cgtcggatcg ttaacttttc
     3301 cgccaggcca gctcttgaga tcgcacaccg ccgcatccgc gtaaatgcag gccgccttgt
     3361 ccggattggc cgtggcccag ttgtagcagt aaaggccgcc tcgactcagt ccgatcagag
     3421 ctaccttcct ggccagcttg tgctcggttg tcaggacctc gtgaaaggca gagcggttcg
     3481 tcgacactga tacaccaaac caccgtgtgg tcactgttcg accgtctcgt cgggaccggt
     3541 gagaccatca gctacaccaa cgcctggccg gacccggtcg ggttctatat gctgggggcg
     3601 gaagtgtcgg caggaccgtg agccccctgc tcctgcccct gctcttgctc ttgctgagag
     3661 gggcggggaa acacggggac cgctcatggg aacgaggatt ggatttggtc tgaggcgcgc
     3721 ctcgcttgac agcatcccgg tcaaccttgt tcgcatcgct cagccaacaa ttccgcgaca
     3781 gcccgttcga tcgcctggtc cgacgtcagg gcgggcgccc agccgagccg ccgcagccgt
     3841 tctatcgagt actgaaaccg cgggacatcg cccggccagc cctgcgcacc gcccgtgtat
     3901 cggatggtcg cgccgggagc cgccgcgcgg ataacagtct gggcaatcca cgacacgatg
     3961 ctcgctgtgc cgtcggtccc gatgttgtag caattgagcc tctcgcatgc ccggtcgacg
     4021 acgaacagca tcgcgtcgac caactcggac acgtgcaaat acggtttctc ctgtcggcca
     4081 tcgcctagaa cctcgagaac ggaggggtcg gcccgcagtt tctgaagcag atcgaaaatc
     4141 accccgtgcg tgccccggct cccgaccacg ttgggaaacc gcagaatcca cgcccgctcg
     4201 agatgcgtct cgagaccggc agaaaccgcc ccttccgacg ccagcttcat cgcgccgtaa
     4261 ttcgagacgg ggaagcacgg cccggtgtct tcgtccagga gacccggccg catgccgtaa
     4321 atggccgaac tcgacgaaaa cgcaagccgg cgcaggccga gtgcgcgcat cagtctgagg
     4381 acggtgaagg tggtgagaaa cgtgtgctcg aaatcgacct gcgggtcgag cgctcccgcc
     4441 cggatatccg aattggcggc cagatgccac accatgtcaa ttggccggcg cgcatgaaaa
     4501 tcccgcaggc gccgcagatt ggtctcgtaa tcgttgatgt cggcttcaaa aaagctgaat
     4561 tcagtgtgct caaaggcctt gcgcaggttg gacgttcgac cgagccgcag gttgtccatg
     4621 ccgaggaccc tccacccgcg atcgagaagc cggtccacca agtgactgcc aatgaacccc
     4681 gccgcgccag tcacccatgc cgttttggat tgtgtcgtca atgccgcaaa tgcaatcgag
     4741 cgctggcgtc tttgtcaacc gcgcaacaga ggtcccatga ccgcccggtg catccgatag
     4801 ttgttggcat tctgtcgccc ggtgagggca ccgggccgac aacggatgga gattgccgga
     4861 ggggccacgc ctgcctgccg cctgccggca ggcaggcagg gcaggcagaa taggcaggcc
     4921 ctcgtcgccc gcggaacagg gtctgcaact ctcatcgcca gggacgaggc cgtctccact
     4981 ccaacgcctt cgtgtgcgcg aacagaacac cacaccacgc cgcgtgccct gcctgcgccc
     5041 caaagcgcag cggcgcgcca ggctcgagat agacagagga atacagcaca ccggcacgct
     5101 gctcccctgg cagactgctc tcaggaccga cagaatgttc ctgaatccct ttctgagcgc
     5161 ggttttggca gatttcggat gtcagactcc ccgctgcgaa atggcgaggc tacgcctccg
     5221 ctttgacact ggtctgcttg acggggacga gcttcttgct ggggtaaccg gcaaccgtct
     5281 cgccgcgcgc gcgcaacgac tccgccaacg catacttgaa ctcctccggc gcgccgtcca
     5341 tcgccccccg cagggcatcg gtcgcctggc tgccgggaac acgcgtcaga gcgcaccgcg
     5401 catcctcgcg cacctcggcg tcggccagga gcgcggccat gggtccgacc gcggcggcgt
     5461 cgccaacttc ggacagcatc cagaccgcgt gccggcgcac gttggcgttg gaatggctca
     5521 gaagcttgat caactcggcc tcgacagcct ttcgctccgg attcgccccg gggcggccgg
     5581 catgccgcgc gatcttctcg atggcgcgct tggccgatcg ggcgatctcg aagtggggat
     5641 cggacatgac ctcggccagg ggcgccacgg cgggcgcgcc caccggtccg gcgccctgcc
     5701 aggcaggacc ccgaacggca tcatcggcgc tcttgatctt tgcgatcagg tcgttgacgg
     5761 ccgcggcatc ggcggctgcg gcggaggccg cgccggtgag aacagcggcg gcagcggtga
     5821 gagtggtttc tgtcttcatg gtgtgagtga ttgattcaga gagcgggaac agagggtcag
     5881 agcgcatacg ggaaacgttg cggacggctc atcaggcgtc gggcggcttc gtctccgacg
     5941 atttcctgtc gcatcggatc ccaggcgagc ttgcgtccga cgatcaggga caggttaccg
     6001 agcacgcaca ggaaggccgt cgcgacgccc ccctcgatgt tcatgatggt cttgcgtccg
     6061 gtcttgatgc cctcgaacca gtcctcgtgg tgatcg
//
LOCUS       DAOQRO010000213         6079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13077_length_6079_cov_4.505146, whole genome shotgun sequence.
ACCESSION   DAOQRO010000213 DAOQRO010000000
VERSION     DAOQRO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13077_length_6079_cov_4.505146"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1534
                     /locus_tag="PKK20_08720"
     CDS             119..1534
                     /locus_tag="PKK20_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00006.1"
                     /translation="MNTIGLDSIDRREFVRTATRALVGAGALNAVAHGLAQDGGERPG
                     RAPGVSVLNPRHRVPVGIIIDDSTCLVNLNRFAVPQFAAVWGAENEYYQKLAWRDWPV
                     EIPDAFVRKFGEWAAENGVKGKYSVVPYPACVGRLDRFLPGWTERELQSSLELVRTVI
                     APHWDIHPEMVSHTRVIDTKTGQPYPECSLKFMENWDWTTGRSADEMADYLAYALRIL
                     KNVGLNCEGVTTPGGFGTGARPQLARGTLEAVRSVFGAEIPHYFRDAFERGSQSVAPR
                     VECAAGLSGPDPQCVVSIVACTGDWTGGWDNTPPGGADKFITPDLQSGRLVEVIQRGE
                     PALLLAHWTGVYWNGLELGFKIFQEVVRRLHARFDNLVWMKLAEIARYWAAKELTTIE
                     RDGNAVRLQAPFGCPAFTVRMEGARGASRPPRLQAGNRLQELEEVAGARELVAGTWCP
                     EANAVLACFDLPKGASHLSLS"
     gene            complement(1701..2531)
                     /locus_tag="PKK20_08725"
     CDS             complement(1701..2531)
                     /locus_tag="PKK20_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNV00007.1"
                     /translation="MTKLPQPKTTTPAGIALAVTAHPDDIEFLMAGTLLLLRQAGWEI
                     HCLNVATGNCGSSQYNAAQTRAIRRREAQEAARRLGAHWHPSLTDDIEIFYEDRLLRR
                     LAAAVRDVRPDIVLTHSPQDYMEDHVNAGRLAVTASFVRGMPNYRTVPRRAAIPGDVT
                     LYHAMPHGLRDGLRRLIIPGAYVDTTEVQSAKRHALAAHSSQKTWLDSSQGMDSYLDA
                     MDQMARSVGKMSRRFAFAEGWRRHSHLGFCAEQADPLQAALGKRYLANPAYERALNCA
                     "
     gene            complement(2577..4190)
                     /locus_tag="PKK20_08730"
     CDS             complement(2577..4190)
                     /locus_tag="PKK20_08730"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNV00008.1"
                     /translation="MKTTAHPTPLWLGCSLAAAIGIAALSVACLPALAQQPTDPTAAA
                     ASEAKAEVEDATETVAVLGKQGSDSPQGFDLHLNPGSGEIVRIGASAHIKQGEKAGQL
                     VVIFGDATMEGEVDGDMVVVCGDAIVDGPVKGDFVTVMGSTKLGPNARLQGDCVVVGG
                     RLDKDPGAQVRHEPVEVSFGILFGWLQPLGYWMKTGLLLGRPLPLTSGLAWTLVILHF
                     AIYFIIFLLMPKPVEACLKTLQTQTLPAFLVGLLTMVLAVPLIVILAASGVGLLIVPF
                     LWLAMLAAKFVGKTAVLQFAGLQLLERFGPQTSPRSLPAFLAGSALVTVLYIVPVLGL
                     IVYGVLLPLGLGAALIAAAAAFRQNDTGQGPVPTVAVPMKTLDTTGTASPSPDTTPSA
                     EASIPDAAPPPSASMDIIQADRAVLPRVGFWLRLAATTLDFILLCWIIPIAGPLFILA
                     WFAYHIGMWTWKGTTIGGIVCQLKIVRIDGRDLDFGVALVRSLGSVLSFMALCLGFFW
                     AGWDKERQAWHDVIAGTTIVKLPKGVSLV"
     gene            complement(4187..4666)
                     /locus_tag="PKK20_08735"
     CDS             complement(4187..4666)
                     /locus_tag="PKK20_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00009.1"
                     /translation="MNPRDSRPDPTWETRLDRELKSLPDRPAPHTLIPRVMAVVQARV
                     CAPWYRRPWWDWPPALKIVSLLGVSGLLGLATWLVMHCSEYPWTAAAASELETWLAPL
                     NPIWTLLTSLASAVALVFKGTGSWILLGAGGLCAIMYLSCVALGTAVYRVASYRRIE"
     gene            complement(4671..5222)
                     /locus_tag="PKK20_08740"
     CDS             complement(4671..5222)
                     /locus_tag="PKK20_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV00010.1"
                     /translation="MSDPLQFEAFMRDYQDMVFTSALRLVGNEADAEDIAQDVFLKAY
                     MHFDQLRHSFTVGGWLKTVTRNLSLNHLTRYRARWRFFSECDDHGESGEHHDFAANLP
                     APDLPTQAVDTADRRNLLESALQKLPPAQRVPLVLYHFENLSYEEIAAKLRISLGKVK
                     TDIHRGRVALRQRLVRNPESATA"
     gene            5900..>6079
                     /locus_tag="PKK20_08745"
     CDS             5900..>6079
                     /locus_tag="PKK20_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011620072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNV00011.1"
                     /translation="MSAAGTSGNRRRPRVSVIFGTRPEAIKLAPVILACRSRTDLECR
                     VCVTGQHRQMLDQVLD"
BASE COUNT         1133 a         1784 c         2058 g         1104 t
ORIGIN      
        1 ttatttacaa cctcaacaac agcaaccctt caggaattta aatgaaataa ccgtgacgcg
       61 cccgctgacg cgagccgacg cgagttgacg cgcgtcccgc atgggttagg ctggcgccgt
      121 gaacaccatc ggtctcgata gcatcgatcg ccgcgaattc gtgcgcacgg ccacccgcgc
      181 gctggtcgga gcgggagcct tgaacgcagt cgcccacggc ctggctcagg acgggggcga
      241 acgccccggg cgtgcgccgg gcgtaagcgt cttgaacccg cgccaccgcg tgccggtggg
      301 tatcatcatc gacgactcga cctgtctggt gaatctcaac cggttcgcgg tcccccagtt
      361 cgccgcggtg tggggcgcgg agaacgaata ttaccagaag ctggcgtggc gcgactggcc
      421 ggtcgagatt cccgatgcct tcgtgcgcaa gttcggcgag tgggcggccg agaatggagt
      481 caagggcaag tacagcgtgg tgccctatcc ggcgtgcgtg gggcgcctcg accgattcct
      541 tcctgggtgg accgaacgcg agttgcagag cagcctcgaa ttggtccgta ccgtgatcgc
      601 gccgcactgg gacatccatc ccgaaatggt cagccacacc cgcgtgatcg acacgaagac
      661 ggggcagcct taccccgagt gttcgctcaa attcatggag aactgggact ggaccacagg
      721 acggtcagcg gacgagatgg ccgattattt ggcatacgcc ttgcgcatcc tcaagaacgt
      781 cggtctgaac tgtgaaggcg tcaccactcc cggcggattc gggacgggag cgcggccgca
      841 gttggcgcgg ggtaccctcg aagccgtgcg cagtgtgttc ggcgctgaaa tcccgcatta
      901 cttccgcgac gccttcgagc gtggttcaca gagtgtcgca ccgcgcgtcg aatgcgccgc
      961 gggactgtcc ggtccggacc cgcagtgcgt ggtcagcatc gtggcttgca caggcgattg
     1021 gaccggtggc tgggacaata ccccgcccgg cggggccgac aaattcatca cgccggacct
     1081 tcaaagcgga cgcttggtcg aggtgattca gcgcggcgag ccggcgcttt tgctggcgca
     1141 ctggacaggc gtttactgga acggcctgga gttgggattc aagatttttc aggaggtggt
     1201 tcgacgactg cacgcccggt ttgacaatct ggtctggatg aaactggctg agatcgcccg
     1261 gtactgggcg gcgaaggaat tgaccacgat tgaacgggac ggaaacgccg tccgcctcca
     1321 agcaccgttc gggtgtcccg ccttcacggt ccgtatggaa ggcgcgcggg gcgccagccg
     1381 gcccccgcgg ctccaggccg gcaatcgcct ccaggaactc gaagaggtag ccggcgcgcg
     1441 ggaattggtc gcgggcacct ggtgtccaga ggcaaacgcc gtgctcgcct gtttcgacct
     1501 gccgaaaggc gcgtcacatt tgtctctgag ctaaccggct ggctcccgtt agatggaccg
     1561 tctggttcgc ctattccagt ttcttcttgg ctgggcgaac ttcatacagg acgcgatctt
     1621 ttcttcattt ttccatgttc gggcgcggtt ttgccagagc ccggatgtca gactccacga
     1681 agccttatga aacatccggg ctaggcgcag ttcaacgccc gttcgtatgc cggattggcc
     1741 aggtagcgtt tcccgagtgc ggcctggagc gggtcggcct gctcggcgca gaatccgagg
     1801 tgcgaatggc ggcgccatcc ctcggcgaag gcgaagcggc gcgacatctt accgaccgag
     1861 cgcgccattt ggtccatggc atcgaggtag gagtccatgc cctgactcga atcgagccag
     1921 gttttctggc tcgagtgcgc ggccagggcg tgacgttttg cggactgcac ttccgtggta
     1981 tcgacatagg cgcccgggat aatcaatcgg cggaggccgt cgcgcagtcc gtgcggcatc
     2041 gcgtgataaa gcgtcacgtc gccgggtatg gcggcgcgcc ggggaacagt ccggtaattg
     2101 ggcatgccgc ggacgaacga ggcggtgacg gcaaggcgac cggcattgac gtgatcctcc
     2161 atgtagtcct ggggcgaatg ggtcaacacg atgtccgggc ggacgtcccg caccgcggcg
     2221 gcgagtcggc gcaggaggcg gtcttcatag aagatttcga tgtcgtccgt gaggcttgga
     2281 tgccaatggg cgcccaggcg ccgggcggct tcctgggcct cgcgccggcg aatggcgcgg
     2341 gtctgtgcgg cattgtattg ggatgagccg cagttgcctg tggcgacatt gaggcagtgg
     2401 atttcccagc cggcctggcg caagagcagg agggtgcccg ccatgaggaa ctcgatgtca
     2461 tccgggtgcg cggtaacggc cagagcgatg cccgcagggg ttgtggtctt gggttgtggg
     2521 agttttgtca tgccgggacg atggcataca agctcggtcc ggtatgtcaa gacggctcag
     2581 accagcgaca cgcccttggg cagcttgacg atcgttgtgc cggcaatgac atcgtgccag
     2641 gcttggcgtt ctttgtccca accggcccag aagaaaccga ggcacagggc catgaacgag
     2701 aggacgctgc ccagcgaacg caccagggcg acgccgaaat cgagatcgcg tccgtcgatg
     2761 cgcacgattt tgagttggca gacgatgccg ccgatggtgg tgcctttcca ggtccacatg
     2821 ccgatgtgat aggcgaacca ggcgaggatg aacaacggtc ccgcaatggg gatgatccag
     2881 cacagcaata tgaaatcgag tgtggtcgcc gcaagtcgca accagaaacc gacgcgcggc
     2941 aggacggcgc ggtccgcctg gatgatatcc attgaggcgc tggggggagg ggcggcgtcg
     3001 ggaatcgacg cctccgcgga cggggtggta tccgggcttg gcgaggcggt tcctgtggtg
     3061 tcgagggttt tcatcggcac cgcgacggtg gggacagggc cttggcccgt gtcgttctgt
     3121 cggaaggcgg cggcggcggc gatgagtgcg gcgccgaggc ccagcggcag caggacgccg
     3181 tacacgatga ggccgaggac cggcacgata tagagcaccg tgacgagggc cgagcctgcc
     3241 agaaaggccg gcaacgaccg cgggctggtc tgcgggccga atcgctcgag aagttggagt
     3301 ccggcgaatt ggagcacggc ggttttgccg acgaacttgg cggccagcat ggcaagccag
     3361 aggaaaggca cgatcaggag gccgacgccc gacgccgcga gaatgacgat caagggcacc
     3421 gcgagcacca ttgtgagcag gccgaccagg aacgccggga gcgtttgtgt ctggagggtc
     3481 ttgaggcagg cctcgaccgg tttgggcatc agtaggaaga tgatgaaata gatggcgaag
     3541 tggaggatga cgagggtcca agccaggccg gatgtcaaag gcaatggccg acccagcaac
     3601 aacccggtct tcatccaata gcccaggggt tggagccagc caaagagaat gccgaaactc
     3661 acctcgacag gttcgtgacg cacctgggcg ccggggtcct tgtcgagtct gcccccgacc
     3721 accacgcaat ctccctggag cctggcgttg ggaccgagtt tggtggatcc catgacggtg
     3781 acaaagtccc ctttgaccgg gccgtccacg atggcgtctc cgcagacgac taccatgtcg
     3841 ccgtcgacct caccctccat ggtggcgtcg ccgaagatca caaccaattg cccggccttt
     3901 tccccctgtt tgatgtgtgc actggcgcca atcctgacga tttcgccgct gccggggttg
     3961 aggtgcaggt cgaatccttg gggcgagtcg cttccctgct tgcccagcac tgcgacagtt
     4021 tcggttgcgt cctcgacctc ggctttggct tcggatgcgg cggcggcagt tgggtcggtg
     4081 ggctgctggg ccagggcggg cagacacgcc acactgaggg cggcgatccc gatcgcggcg
     4141 gcgaggctgc agcccagcca caagggagtg ggatgtgcgg tcgttttcat tcaatcctcc
     4201 ggtaagaagc aacacggtag acggcggtgc cgagggccac gcaggagaga tacatgatcg
     4261 cgcacaggcc tcctgcgcca agaaggatcc aactgcctgt gcccttaaac acaagcgcca
     4321 cggcgctggc caaggatgtc aggagagtcc agatcgggtt caacggagcc agccaagtct
     4381 cgagttcgct ggcggccgcg gcagtccatg gatactcgct gcagtgcatg acgagccagg
     4441 tcgcaagacc gaggagaccg gacaccccca gcagggagac gatcttgagg gcgggcggcc
     4501 agtcccacca gggccgccgg taccaaggcg cgcagacccg ggcctgcacg acggccatca
     4561 cgcggggaat gagtgtgtgc ggcgccgggc gatccggaag gctcttgagt tcgcggtcca
     4621 ggcgtgtttc ccaggtcggg tcgggtcttg aatctctcgg gttcatacgt tcaagcagtg
     4681 gccgattccg ggttgcgcac gagtctttgg cgcagcgcca cgcgtccgcg atggatgtcg
     4741 gtcttcacct tgccgagcga gatgcgcagt ttggccgcga tttcctcgta gctgaggttt
     4801 tcgaagtgat agagaaccag cgggacccgt tgggctgggg gcagtttttg cagcgcggat
     4861 tcgagcaggt tccgccggtc cgcggtgtcg acagcctggg tgggcaggtc cggggcgggt
     4921 aggttggcgg cgaaatcgtg gtgttctccg ctctcgccgt ggtcgtcgca ttccgagaag
     4981 aaccgccagc gggcgcggta gcgtgtcagg tgattcaacg agaggttgcg ggtgacggtc
     5041 ttgagccagc cgcccaccgt gaagctgtga cgcaactggt cgaagtgcat gtaggctttc
     5101 aggaacacat cctgcgcgat atcctcggca tcggcctcgt tcccgaccag ccgcaacgcc
     5161 gacgtaaaca ccatgtcctg gtagtcacgc ataaaggcct cgaattggag tgggtcactc
     5221 atccgtccag tcatgcgtcc ggctgtccca gactaattcg agttcattgt cacctccagg
     5281 acccgcatcg aaagccgaaa gttgcaaaaa gttcaaagca aaggctgtcg acaaggcctc
     5341 gggaaaaggc aagagcctta cggcaatggt ctgagaaaca gatcgtcatc gtcgcccaag
     5401 gccctcgcca gaccgcctcg aacagcgcct cgagccccaa acgcgagcgc tatggaattc
     5461 cgggtccatc cgcgacaaat cgcggtgtca gcccgcccct catgaccctt cgtttcaaga
     5521 agaccggcgc tgatgccgcc gcccgaagag aagccagcgc cgaagagttc ggcacgaggg
     5581 ttgtcctctg gacccgttga tggatggtat gttccaacag agcgggtcgc cacggacgaa
     5641 acacggatga tacgccgatc gggcgaggag agaaggtgcg ctcgtgccga tcggttcttg
     5701 gccttcgact ggcgagtcgc aagcggtatt ctcgcgaatg gaacgggcct catcccacgt
     5761 tcgatccgtg tttgacccat gttccatccg cggctcccat tccatccgtg tttcatccgt
     5821 gctcgatccg tggctcgcgt tccatacgcg gcagaaattc ctttcgcggg cggacaaggc
     5881 ttcgtagcgt ggcgcccgca tgtcagcggc tggcaccagt ggaaatcgga gacgtccgag
     5941 agtgtccgtg attttcggca cgcgcccgga ggccatcaag ttggctccgg tcattttggc
     6001 gtgccgttcc cgaacggacc tcgagtgtcg ggtttgtgtg acagggcagc atcgccagat
     6061 gctcgatcag gtgctcgat
//
LOCUS       DAOQRO010000214         6078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13083_length_6078_cov_4.658476, whole genome shotgun sequence.
ACCESSION   DAOQRO010000214 DAOQRO010000000
VERSION     DAOQRO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13083_length_6078_cov_4.658476"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKK20_08750"
     CDS             complement(<1..472)
                     /locus_tag="PKK20_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV00012.1"
                     /translation="MLAAQSTAAGAAVRAAPAPVSPHSWRRIVGPADGFLKSVARRLE
                     DQVSGFEPDIAGYVRYALASQGKQLRPALLALSAKPIGALTDDLITVATIIEMVHLAT
                     LVHDDVMDEARVRRRRPTLSVRCGNAISVLAGDCLFAHALESTEIALPHRTDRVG"
     gene            complement(624..1118)
                     /locus_tag="PKK20_08755"
     CDS             complement(624..1118)
                     /locus_tag="PKK20_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00013.1"
                     /translation="MNLDDAQKQQVIAWLQEGATLAELQKRLEATFGLRLTYRDVKFL
                     VSDLEVLPKDVEPTAPTDLTSRDLKAPGALHAPAKGGAGEPETAPTPPAARVRISVDQ
                     LTRTGAVVSGNVTFSDGKSAVWSIDQMGRLGIAPQEKGYRPSSGDLQEFQMLLEQELA
                     RHGF"
     gene            1423..2424
                     /locus_tag="PKK20_08760"
     CDS             1423..2424
                     /locus_tag="PKK20_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNV00014.1"
                     /translation="MTTTTESQTDERGEFRRELACVWRQMSDKGLLLSLAAAWFALFH
                     FLGNSTFGYVDSPSLFGWLWGAYSSSSFPGGPIVKLLNADDGYCVLIPFLVLGLFWSR
                     RQQWLAIHRETWWPGLVGLALATVLHVLGYLVQQPRVSLVAFLGGGYALIALVWGWRF
                     AAGVFFPYVLLVFCVPLGTVSDPITVPLRQVSTDVSVVIVRDVLGIPVIQQGVQISDP
                     RGAYTYEVAAACSGIRSLIALFALTTIYGFLTFTRLWKRLLVILLVVPLTLAGNILRL
                     VCIVIASEAFGRSAGEFVHDWFGFVTFGLALLVLFGVGNWLREPASARVPGGLPQAA"
     gene            2421..3158
                     /locus_tag="PKK20_08765"
     CDS             2421..3158
                     /locus_tag="PKK20_08765"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HNV00015.1"
                     /translation="MKARQYILFALALAVIVGGGGLLVRLKSIQRLGAPGIKVSPIEG
                     SDRLRIELPERVPLYESTGMEPTRAELEMLPEDTTFAKRLYRAADGFQIMMNVVMMGT
                     DRTSIHKPEFCLTAQGWRIERQETDMLPVYRPHRYDLPVQRYTTKAEWEQEGGRRVPW
                     SGVYVFWFVAENKLTASHWVRVGWITRELLRRGVLPRWAYIGCFAACPPGQEEATFRR
                     MRQFLTASVPDFQTAVPPALAGGGADR"
     gene            3171..4568
                     /locus_tag="PKK20_08770"
     CDS             3171..4568
                     /locus_tag="PKK20_08770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNV00016.1"
                     /translation="MLSRHTRLRTQIQIVLDAVLLGLSLYLAHQLRTHWRFEVFGGTR
                     DIRSLESYMWLFPLVMVGGPFLLRSIGFYNRPFLPSRRLTAWQLIKGCVYATIGLVIV
                     IFVVRQGGEIGRSVIILFGLISFGVISIKEEIVLQWRMGRLGGAPMEKRLILVGTTED
                     TVRLRQDLSRSQREELNIVAQLSINERPVSDLVELMHVHSANGVVLTAKHTYLGQIEQ
                     AIELCEREGVEVWLLADFFKTQISRTGVDDFYGRPMLVFRSGPDTSWPLAVKRLIDFW
                     GALLLLVLLGVPMLLVGLIIKLGSPGSALFRQQRSGLNGRPFTMLKFRSMVTDAEQRK
                     QELALLNEMSGPVFKVTNDPRVTPVGRFIRKWSIDELPQLINVLRGEMSLVGPRPLPV
                     AEVARFDDLAHRRRLSVRPGITCLWQVSGRSEVRDFRDWVRLDLEYIDNWSLWLDLNI
                     LLRTIPAVLTAAGAK"
     gene            4697..>6078
                     /gene="pilM"
                     /locus_tag="PKK20_08775"
     CDS             4697..>6078
                     /gene="pilM"
                     /locus_tag="PKK20_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNV00017.1"
                     /translation="MLGAKSFLCIDFGAGSLKLAEFDVGDSGALRLVRYALKPLGLAG
                     AQDAAREGVVLRGLQELLSDKAIAARTCNVCAPGFHVFSKFVKLPPVDSSKIGQIIQY
                     EAQQNVPFPLEEVVWDYQILGTLPSGELEVLLVAIKSDVVESLFRSAEGVGLRLELVD
                     VSPAALCNAFRYNYGDLEGCSMLLDIGAKTSNVLFFDKGKVFSRCINIGANSITQEFA
                     AEAKLPFVEAEKLKIAEGFVSLGGAYEEPDNPRQAAISKIARQVMTRLHIQVNQTIQF
                     FRSQQGGTAPQRVYLAGGASIMPYTREFFQEKLNLTVEYFNPFRNIEIDPGVNLEELA
                     KVAHAYGEVVGLGLRNPTQCPVELNLIPKSILKRQELNQKKPYFIAAAASLIIVVFIF
                     GWFFDKLAKEKQGAIDARAPKVEGLRMKKQALDTAISSKDKSFKEAEQYAEWMSERFR
                     WADIMTALDQA"
BASE COUNT         1012 a         1800 c         2011 g         1255 t
ORIGIN      
        1 gccccaccct gtccgtccga tgcggcaatg cgatctcggt gctttccagg gcgtgcgcga
       61 acaggcaatc cccggcaagc accgagatcg cattgccgca tcggacggac agggtggggc
      121 gtcgccgccg gacccgggct tcgtccatga catcgtcgtg gaccagcgtg gccaagtgga
      181 ccatctcgat gatggtggcc acggtgatca gatcgtcggt gagagcgccg atcggtttgg
      241 cgctcagggc gaggagggcc gggcggagct gcttgccctg gctcgccaaa gcgtagcgga
      301 catagccggc gatgtcgggc tcaaagccgc tgacctggtc ctccaatcgc cgggccacgg
      361 atttgaggaa gccgtcggcg ggaccgacaa tgcgccgcca ggaatggggc gagacggggg
      421 cgggagcggc tctgaccgcg gctcccgccg ctgtggattg agcagccagc acgttggccg
      481 ggaatctaga gttgtgcgct ggacaagtca aacggttacg cagccggggt ctaacccgca
      541 tcgcagtcgt cctgcgagct ctgaccgttc tgtgcgccga aggggtgcgg aattaggcgg
      601 ggtagagacc gcgcggcccg gattcagaag ccgtggcgcg ccagttcctg ctccagtagc
      661 atctggaatt cctggagatc gcccgacgac ggccggtagc ccttctcttg cggcgcgata
      721 ccgaggcggc ccatctggtc gatggaccag accgcactct tgccatcgct gaaggtgacg
      781 ttgccgctga cgacggcccc cgtccgggtc aactgatcca cgctgatccg gacgcgggcg
      841 gccgggggcg tcggggccgt ctcgggctcc ccggcgccgc ctttggccgg cgcatggagc
      901 gccccgggtg ccttgagatc cctgctggtc aagtcggtcg gcgccgtcgg ctcgacatcc
      961 ttggggagga cctcgaggtc gctgacaagg aacttgacgt cccggtaggt gaggcgcagg
     1021 ccgaacgtcg cctcgagtcg tttctgcagt tccgccaagg tggcgccttc ctgaagccac
     1081 gcgatgacct gttgtttctg ggcgtcgtcc aaattcatag cgtactggag gccattcggc
     1141 gcgatttggc gcattcaggc aactgatttc ttgcctcttc tttcttcgaa gtcttcatcg
     1201 agttcgcccg cgcttccagg caaagccgcc tcgtctccgg ctgctgcgat ttatatgctt
     1261 ccgtccgtcc gcgcgttgcc gcgttgagtt ccgcgataat tttgattgcg ataatttggt
     1321 tgagcgatgc gggggagtgg tgtcagatgg ggtgaatcgc gtctgcgcgc acgacgagag
     1381 gaacgggcaa tgcgcgcggc gggacgcgag cggcattccg ggatgacgac aacaacagag
     1441 agccagaccg acgaacgggg tgagttccgg cgggaactcg cctgtgtttg gcggcagatg
     1501 tccgacaagg ggctgctcct gagcctggcg gccgcatggt ttgccctgtt ccatttcctg
     1561 ggcaactcga cttttggcta cgtcgacagt ccttccctgt tcggctggct ctggggcgcg
     1621 tacagttcga gttcgtttcc cggcggaccg attgtgaagc tcctcaacgc tgacgacgga
     1681 tactgcgtcc tgatcccgtt cctcgtgctt ggtctgttct ggtcgaggcg tcaacaatgg
     1741 ctggcaattc accgggagac gtggtggccg ggcttggtcg ggctggcgtt ggccacggtg
     1801 ctgcacgtgt tgggctacct tgtgcagcag cctcgagttt cgctcgtggc gtttctgggg
     1861 ggcgggtacg cgctgatcgc attggtctgg gggtggcggt tcgccgccgg cgtgttcttc
     1921 ccctatgtgc tgcttgtctt ctgtgttccc ctggggacgg tcagcgatcc gatcaccgtt
     1981 ccgctccggc aggtttcgac ggacgtttcg gtggtgattg tgcgggatgt gttgggcata
     2041 ccggtgattc agcagggggt gcagatcagc gatccgcgcg gcgcgtacac ttacgaagtc
     2101 gccgccgcat gcagcggcat tcggagtctg atcgccctgt tcgccctgac cacgatttac
     2161 gggtttctga cattcacgcg cctctggaag cgtctgctgg ttatcctgct cgttgttccc
     2221 ctgaccctgg cgggcaacat cctgcggctg gtctgcatcg tgatcgcgtc cgaggcgttc
     2281 ggacggagcg cgggggagtt cgtgcacgat tggttcggat tcgtcacgtt cggactcgcg
     2341 cttctggtgc tcttcggcgt cgggaactgg ctgcgcgagc cggcgtcggc ccgggttccg
     2401 ggaggattgc cccaagccgc atgaaggcgc gccaatacat ccttttcgcc ctggccctgg
     2461 cggtgatcgt gggcggcggc ggccttctgg tgcggctcaa gtcgattcag cggcttgggg
     2521 cgccggggat caaggtcagc ccgatcgagg ggagcgatcg gctccggatc gagttgccgg
     2581 agcgcgtgcc gctctacgag tcgacgggca tggagccgac gcgcgccgag ctcgagatgt
     2641 tgcctgagga tacgaccttt gccaagcgtc tgtatcgtgc cgccgacgga tttcagatca
     2701 tgatgaacgt cgtgatgatg ggaaccgacc gcacgagcat ccataagccg gagttctgtt
     2761 tgacggcgca gggatggcgg atcgagcggc aggagacgga catgctgcca gtctatcggc
     2821 cgcatcggta cgatctcccg gtccagcgtt acacgaccaa ggcggagtgg gagcaggaag
     2881 gagggcgtcg agtgccctgg agcggtgtgt acgttttttg gtttgtagct gaaaacaagc
     2941 tgaccgccag ccattgggtg cgggtgggat ggatcacgcg cgaactgctc aggcgcggcg
     3001 tgctgccgcg gtgggcctac atcggctgct tcgcggcctg tccgccgggg caggaggagg
     3061 cgaccttccg gcgcatgcgg cagtttttga ccgcctcggt gcccgatttt cagactgccg
     3121 tgcctcctgc tctggcggga ggaggcgcgg accgttagcg aggaagcctc atgttgagcc
     3181 gccacacacg ccttcgaacc cagatccaga tcgtgctgga cgcggttctt ttagggctga
     3241 gcctctacct ggctcaccag ttgcgcaccc attggcgctt cgaggtgttc ggcggaacca
     3301 gggacatccg gagtctcgaa tcctacatgt ggctgttccc gctcgtgatg gtcggcgggc
     3361 cgttcctgct cagatcgatc ggtttctaca accgtccctt tctgccgtcg cgccggttga
     3421 cggcgtggca gttgatcaag ggctgcgtgt acgcaaccat tgggctggtc atcgtgattt
     3481 tcgtggtgcg acaggggggt gagattggcc gctcggtcat cattcttttc gggttgatca
     3541 gcttcggggt tatctcgatc aaggaggaga tcgttctgca gtggcgcatg gggcgtttgg
     3601 gaggcgcccc gatggagaag cgactgatcc tggtggggac gaccgaggat accgtgcgcc
     3661 tgcgccagga cctctcgcgc agtcagcgcg aggagctgaa cattgtggcg caattgagca
     3721 tcaacgaacg tcccgtgagc gacctggtcg agctcatgca cgtgcattcg gccaacggcg
     3781 tggtcctgac ggccaagcac acctaccttg gacaaatcga gcaggcgatc gagttgtgcg
     3841 agcgcgaagg cgtcgaggtc tggttgctcg ccgacttctt caagacccag atttcccgca
     3901 cgggtgtgga tgatttctac ggacgtccca tgctcgtctt ccgttccggg cccgacacct
     3961 catggcccct ggcggtcaag aggctcatcg atttctgggg ggcgctcctg ctcctggtcc
     4021 tgctgggagt cccgatgctc ctggtcggcc tgatcatcaa gctcggctcc ccgggctccg
     4081 ccctgtttcg acagcaacgc agcgggctca acgggcgacc gttcacgatg ctgaaattcc
     4141 gatcgatggt gaccgatgcc gagcaacgca agcaggagct ggcgttgctc aacgaaatga
     4201 gcggcccggt cttcaaagtc acgaatgatc cccgcgtgac accggtgggg cgtttcatcc
     4261 gcaagtggag catcgacgaa ctgccgcaac tcatcaacgt gctccggggc gagatgagcc
     4321 tggtgggccc acgccccctg ccggttgccg aagtcgcccg gttcgacgac ctggcccacc
     4381 ggcggcgctt gagcgtgcgg ccggggatca cgtgtctttg gcaggtgagc gggcgcagcg
     4441 aggtccggga tttccgggat tgggtgcggc tcgatctcga gtatatcgac aactggtcac
     4501 tatggctgga cctcaacatt ctgcttcgga ccatacccgc ggttctgacc gccgcggggg
     4561 caaaatgatc atgaagacga cttttgtgtt tgaccggctt catcatttcc agtaaccaca
     4621 gcgcgctttc tcggttttgg ctcgatactg gctgtcataa acccggaatc gatggaacca
     4681 tgatcgacgg aatcacatgt taggtgcgaa atcattcctc tgtatcgatt tcggcgcggg
     4741 cagcctgaag ctggccgaat tcgatgtggg tgactccggc gcgctgcgtc tggtccgtta
     4801 tgccctcaag ccgctcgggt tggccggcgc tcaggacgcc gcgcgcgagg gtgtcgtgtt
     4861 gcgaggcctc caggaactgt tgtccgacaa ggcgattgcg gcccggacat gcaacgtctg
     4921 cgccccgggt ttccatgtct tttcgaagtt cgtcaagctc cccccggtcg attcgtccaa
     4981 gatcggccag atcatccagt acgaggcgca gcagaacgtc cccttcccgc tcgaggaagt
     5041 ggtctgggat taccagatcc tgggcacatt gccgtcgggc gaactcgaag tgctcctggt
     5101 cgccatcaag tcggacgttg tcgagtccct ctttcggtcg gcggaagggg tgggcctgcg
     5161 gcttgaattg gtggatgtct cgccggcggc cttgtgcaac gccttccgtt acaactacgg
     5221 cgacctggaa ggctgctcga tgctgctgga catcggggca aagaccagca acgtgctgtt
     5281 cttcgacaag ggcaaggtct tctcacgctg cattaacatc ggcgccaact cgatcactca
     5341 ggagtttgcg gccgaggcca agctcccgtt cgtcgaggcg gagaaactta agatcgcgga
     5401 gggctttgtc agtctgggtg gcgcgtacga ggagcccgac aacccgcgcc aggccgccat
     5461 ctcgaagatt gcccggcagg tgatgacccg tcttcatatc caggtcaatc agacgatcca
     5521 gttcttccgc agccaacagg gcggtacggc ccctcagcga gtctacctgg caggcggcgc
     5581 gtcgatcatg ccctacacgc gcgagttctt tcaggaaaag ctcaacctca ccgtcgagta
     5641 tttcaaccca ttccgcaata tcgagatcga tccgggcgtc aacctcgagg agttggccaa
     5701 ggtcgcgcac gcctacggcg aggtcgtggg actcggattg cgcaatccga cccagtgccc
     5761 cgtcgaactc aatctcatcc ccaagagcat cctcaagcgg caggaactca accagaaaaa
     5821 gccgtatttc atcgccgccg ccgcaagcct gatcatcgtc gtcttcattt ttggctggtt
     5881 cttcgacaag ctggccaagg aaaaacaggg ggccatcgat gcccgcgccc cgaaggtcga
     5941 ggggctgcgg atgaagaagc aagccctcga tacggccatc tcgagcaagg acaagtcgtt
     6001 caaggaggcc gagcagtatg ccgagtggat gtccgagcgc ttccgctggg ccgatatcat
     6061 gaccgcgttg gaccaggc
//
LOCUS       DAOQRO010000215         6071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13110_length_6071_cov_6.420711, whole genome shotgun sequence.
ACCESSION   DAOQRO010000215 DAOQRO010000000
VERSION     DAOQRO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13110_length_6071_cov_6.420711"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKK20_08780"
     CDS             complement(<1..798)
                     /locus_tag="PKK20_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV00018.1"
                     /translation="MSTRPLRVDLDLVKKYNVPGPRYTSYPPATLFTDRVPAGEVLDN
                     IARNNAASSDLSLYFHIPFCETLCWFCGCTTVITTDPRKSAAYVETLGREMERMRPLL
                     DRGRRVVQVHFGGGSPTFCRPEQLSRLGVLIHRHFPFAEGIEAGVEIDPRRLTRDHLV
                     ALREIGFNRASIGVQDNHPEVQKAVHRMQPPELTAQAAGWVRETGFSSLNIDLIYGLP
                     FQTVASFDRTLDDMLELAPERFAVFSYAHVPWMKPAQKIVEARHLPTP"
     gene            complement(795..1907)
                     /gene="hemE"
                     /locus_tag="PKK20_08785"
     CDS             complement(795..1907)
                     /gene="hemE"
                     /locus_tag="PKK20_08785"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNV00019.1"
                     /translation="MPDSLAETGQDREPSDPPCRLATRLTELPGTNRQRFLDACRCRT
                     VDRPPFWLMRQAGRALPEYRQLKQRHSFLELVRTPDLAAEITLQPIRRFQYDAAILFS
                     DILVIAEALGQPYGFRDTGGIEMAFAIRSTAEIDRLEVSAVRERLAYMAEALRLVKRQ
                     LGGQTALIGFAGAPWTLSNFMLEGGSAPEFTRARALLENEPKLYHHLAEKLSAAIADC
                     LLMQIDAGAEAVQIFDTLAGSLGDARFVEASARWLGEIIERIGGRVPVILFAKGINQS
                     WSVLAQTGADVLSVDASVRLSSLRAQLPPRIAIQGNLDPAWLRTTPAQVRAATRQMLE
                     EMRGCRGYIFNLGHGVPPDAALEHLDALAQTVRGFA"
     gene            complement(1948..2238)
                     /locus_tag="PKK20_08790"
     CDS             complement(1948..2238)
                     /locus_tag="PKK20_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00020.1"
                     /translation="MRTKSVAIACAAALALIGFTPASALAGPSPEAVVADVLVARPAC
                     LAVTAVGSAFFVVTLPFAAISKSTKKTAEALVIKPAKATFTRPVGDLDALID"
     gene            complement(2415..3287)
                     /locus_tag="PKK20_08795"
     CDS             complement(2415..3287)
                     /locus_tag="PKK20_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV00021.1"
                     /translation="MPKQAWAVAMVLTALVALGLGGLAYWHRGLELAEERNRATQARS
                     EATRARSEAIEAHAGAAEAEMAAATNRTRVAELETALAQAQQTLDDLRRQTEQHRQNQ
                     QSLEAEFRRALESKEITISELQGKLTVDILDRILFDSGEAELKTEGQEILRKVAAVLA
                     QHPKRRIHVIGHTDNVPIRASARARYPSNWELSTARALAAVRFLIDEAGVDPQRLGAV
                     GYGEFRPLADNATPEGRAKNRRIAIVVLSDELVGSDATPPAPLSPPPPAETPDPGSTN
                     APPVIPSPETRANS"
     gene            3610..4743
                     /locus_tag="PKK20_08800"
     CDS             3610..4743
                     /locus_tag="PKK20_08800"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNV00022.1"
                     /translation="MNVVIAPDKFKGTLTATEAAEAIAAGWRLERPQDTVRAFPISDG
                     GDGFGDLLARHLNAEERTVDTVNAAHEPIRAPWWWEPRSRMAIIESARIIGLAMLPRG
                     RFHPFNLDAFGLGAVLRAAAAAHPRMCLMGIGGSATNDAGFGLARAIGWQFHDRRGKP
                     IEDWTRLPELERIERPERALRLRQVLVAVDVTNRLMGVRGATRVYGPQKGIRPEDVKP
                     AERCLRRLVRVLTRQFHFGVPPHHLPGSGAAGGLGFGLRCFASARLEPGFRLFARLSG
                     LLDEIRKADLVITGEGAIDLTTVAMGKGVGQLMRICGRLRRPCIALAGQVHVSRFRQP
                     GLVGLYGMAPTLTTAEEAMSRPAHWLAELGRFSARAWPAQTKP"
     gene            complement(4775..5920)
                     /locus_tag="PKK20_08805"
     CDS             complement(4775..5920)
                     /locus_tag="PKK20_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation inhibitor"
                     /protein_id="HNV00023.1"
                     /translation="MDKKRSTRKAVDGMQVIRLPNGGFQETFITVPAIGSEEPASLFQ
                     RLYDFLADNAQLRIVREDVFGLDIDPFKIRHARGYRVAGIEWPMTWVEEGNGGGGPIA
                     GVQVHAVSGIDVHPINLEGHTVGTLFEDQHARYCVMGGLHPESIEETREEQAWETFEI
                     MESALELAGMTFSNVFRTWFYLDDILDWYDEFNGVRNQFFLERKVCDGLVPASTGIGG
                     GNSAGTAVIAGLLAIQPKGPTVRLQAIPSPLQCPAVEYGSSFSRAVELELPGQRRLYV
                     SGTASIAPGGETCHVGDLRKQVALTMDVVAAILESRRMGWGDVTRMIGYFKHGEDASE
                     FDRYCRDHKLPKIPVIIAKNDICRDDLLFEIEADAAKLGGPPLAGAN"
BASE COUNT         1067 a         1978 c         1968 g         1058 t
ORIGIN      
        1 gggcgtgggc aggtgcctgg cttcgacgat cttctgcgcc ggcttcatcc agggcacatg
       61 cgcgtagctg aacacggcga accgctcggg cgccaactcg agcatatcat cgagtgtccg
      121 gtcgaagctc gccacggtct ggaaagggag gccgtagatc aggtcgatgt tcagcgaact
      181 gaacccggtc tcccgcaccc agccggccgc ctgggcggtg agttcgggcg gctgcatccg
      241 gtgcaccgcc ttctggactt cgggatggtt gtcctgaact ccgatcgatg cccggttgaa
      301 cccgatctcg cgcaaggcga ccaagtgatc gcgcgtgagc cggcgggggt cgatctcgac
      361 cccagcctcg atcccttccg cgaaaggaaa atgccggtgg atcaggacgc ccaggcgact
      421 gagttgctcg ggacggcaga acgtgggcga tccgccgcca aagtgcacct gaaccacccg
      481 acggccgcgg tccagaagcg ggcgcatccg ctccatctcg cgtccgaggg tttcgacgta
      541 cgcggcgctc ttgcgcgggt cggtggtgat gacggtcgtg cagccgcaaa accagcagag
      601 cgtctcgcag aatgggatat ggaaatagag cgacagatcg ctggacgccg cgttgttgcg
      661 cgcgatgttg tccagcacct cacccgccgg cacgcggtcg gtgaaaagcg tcgcgggcgg
      721 gtacgacgtg tagcgcggac ccggcacgtt gtacttcttc accagatcga gatcgacgcg
      781 caacggtcgg gtgctcatgc gaaaccccgc acggtctggg ccagcgcatc gaggtgctcg
      841 agcgccgcat ccggcggcac gccgtggccc aagttgaaga tatatccccg gcagccgcgc
      901 atctcttcga gcatctggcg cgtcgcggca cgcacctgag cgggagttgt gcgcagccac
      961 gccgggtcca ggtttccctg tatggcgatt cggggcggga gttgcgcgcg caggctgctc
     1021 aggcgcaccg aggcatccac gctcaggaca tcggcgcccg tttgcgccag gacggaccag
     1081 gactggttga tgcccttggc aaagagaatg accggcacgc gtccgccgat gcgctcgatg
     1141 atctccccca gccaccgggc cgaggcctcg acgaaccgcg cgtcgcccag agacccggcc
     1201 agcgtgtcga aaatctgaac tgcctccgcc ccggcatcga tctgcatcag gaggcagtcc
     1261 gcgatcgctg cgctgagctt ttcggcaagg tggtggtaga gcttgggctc gttttcgagc
     1321 agcgctcggg cgcgcgtgaa ctcgggtgcg ctgcccccct cgagcatgaa attcgagagc
     1381 gtccatggcg cccccgcgaa cccgatgagg gcagtctggc cgccgagctg ccgtttgacc
     1441 aggcgcaggg cctcggccat gtaggccagg cgctcgcgca ccgcggacac ctccaaccga
     1501 tcgatctccg cggtcgatcg gatggcaaag gccatttcaa taccgcccgt gtcccgaaat
     1561 ccatagggct ggcccagcgc ctccgcaatc acgagaatgt cgctgaacag gatcgcggca
     1621 tcgtattgga aacggcggat cggctggagg gtgatttccg cggcaagatc aggcgtccgc
     1681 accaactcga ggaacgaatg cctctgcttc aactgccggt actcgggaag tgcgcggccc
     1741 gcctgccgca tcagccagaa gggcgggcga tccaccgtcc ggcagcggca ggcatcgagg
     1801 aagcgttggc gattcgttcc cggcaattca gtaaggcgcg ttgcgagccg gcaaggcggg
     1861 tcactcggtt cacgatcctg gcccgtctcc gcgagcgagt caggcatcgc ctagtcgacg
     1921 acgactctgg gcgccgaatg caaggcctca gtcgatcaga gcatccagat cgccgaccgg
     1981 gcgcgtgaac gtcgccttcg ccggtttgat caccagcgcc tcggctgtct tcttggtgct
     2041 cttcgaaatc gcggcgaacg gcaaggtgac cacgaagaag gcgctgccca ccgcggtcac
     2101 cgccaggcag gccggtcgtg cgaccaggac atcggccacg accgcttcgg gcgatggacc
     2161 ggcaagcgcg gaggccggcg tgaaaccgat cagagccagt gcggcagcgc aagcgatggc
     2221 aacagacttg gttctcatga ggatgcaaca ctagcccatc gcaaggcgga ctgtcgaaca
     2281 gattgtttac gacttcacct ccggccaggg ccaatgcatg aagtggagcg gcaggcaggg
     2341 ccccgtcgcc ggcaaatacc gtcttcactc gcgttggccg gggacgagcc gtccccgctc
     2401 cggcctttgc ggcttcatga attggctctg gtctcgggcg agggaattac agggggcgca
     2461 ttcgtcgagc cgggatccgg cgtctcggcg ggcggcggag gcgacaaagg cgcaggcggc
     2521 gtggcatccg aaccgaccaa ctcgtcggac aagaccacga tggcgatgcg gcgattcttg
     2581 gctcggcctt cgggcgtggc gttatcggcc agaggacgga actcgccgta gccgacagcg
     2641 cccaaccttt gcgggtcgac ccccgcctca tcgatcagaa agcgcaccgc cgccaatgcc
     2701 cgcgcggtcg acagttccca attgctcggg tagcgggcgc gagccgatgc ccggatgggg
     2761 acgttgtcgg tgtgtccgat gacgtgaatc cggcgtttgg gatgctgggc caggactgcg
     2821 gcgaccttgc gcaggatttc ctgcccttcg gtctttagct ccgcctcgcc cgaatcgaac
     2881 aggatgcggt cgagaatatc caccgtcagc ttgccctgaa gctcggagat cgtgatttcc
     2941 ttcgattcga gggcgcgccg gaactcagct tcgagcgact gctggttctg acgatgctgt
     3001 tcggtttgcc gacgaagatc atcgagagtc tgttgggcct gcgcgagtgc tgtttcgagt
     3061 tcggctacgc gggtccgatt cgtcgcggcg gccatctcgg cctcggccgc gccggcgtgg
     3121 gcctcgatgg cttcggatcg ggcccgggtc gcttcggatc gggcctgagt ggcccggttg
     3181 cgctcctcgg ccagttcgag gccccgatgc caatacgcca gaccccccag gcccagagcc
     3241 accagagccg tcaggaccat cgccactgcc caagcttgtt tcggcatacg cagctccaag
     3301 aattggccgg acgcgcccgg gcgtaaacaa gaattctaac ggaggcgcga cgaaggagca
     3361 tagccttgac ctctgtgatt gaggagcaac gaagccatcg agccaaagcc ccccagccct
     3421 ccggggcggg gcgccaccgg gccatctgcg tcgttcctcg gtcgggcaag tgtcatcggt
     3481 caaggattcc acacccccct ctttcggagt tcccgcggcg gtgaccgcgc ggtgctgacg
     3541 atcgcacggt tgattgcggg gttgacttgc gctcgatgca ctcggctact cttgttcgag
     3601 agatctctta tgaatgtcgt catcgccccc gacaagttca aaggcacgct cacagccacc
     3661 gaggccgccg aagccatcgc cgccggctgg cggctcgagc gccctcagga caccgtgcgc
     3721 gcgttcccga tcagcgacgg cggcgacggt ttcggcgacc tgctcgcgcg acatctcaac
     3781 gccgaggaac gaacggtcga caccgtcaac gccgcacacg aaccgatccg cgcgccgtgg
     3841 tggtgggaac cgcgcagccg catggcaatc atcgaatcgg cccgaatcat cggtttggcg
     3901 atgctgcctc gagggcggtt tcatccgttc aatctcgatg ccttcggcct gggagccgtg
     3961 ttgcgggccg ccgccgccgc gcatccccgg atgtgcctga tgggcatcgg cggcagtgcc
     4021 accaacgatg ccggattcgg cctcgcccgc gcgatcggat ggcagttcca cgacaggcgg
     4081 ggaaagccga tcgaggattg gacgcgcctg cccgagctgg agcgcatcga acggcctgaa
     4141 cgcgcgctgc gtttgaggca agtcctggtg gccgtggacg tcacgaaccg cctgatgggt
     4201 gtgcgcgggg cgacccgggt ctatggaccg caaaagggga ttcgccccga agacgtcaaa
     4261 ccggcggaac gctgtctgcg gcggctggtg cgagtcctca cacggcagtt ccacttcggc
     4321 gttcctccgc accatctgcc gggctcaggc gcggcgggcg gcttgggatt cggcctccgc
     4381 tgcttcgcct cggcccgcct cgaacccggc tttcgcctgt tcgcccgact gagcgggctg
     4441 ttggatgaaa tccgcaaggc agacctggtc atcacgggcg agggcgcgat cgacctgact
     4501 accgtggcga tgggcaaagg cgtgggacaa ctgatgcgca tctgcgggcg gctgcggcgt
     4561 ccctgcattg ccctcgccgg ccaggtgcac gtctcccggt tcaggcaacc gggcctggtc
     4621 ggcttgtacg gcatggcccc gaccctgacc acggctgaag aggccatgtc gcgtccggcg
     4681 cattggctgg cggaactggg ccgattcagc gcccgcgcct ggcccgctca gaccaagccc
     4741 tgagacgtgg gttcaggcag aagttcggtg cggctcagtt ggcccccgca agaggcggtc
     4801 cgccgagctt tgcggcgtcc gcctcgatct cgaagagcag gtcgtcccgg cagatgtcgt
     4861 tcttggcgat aatgaccggg attttcggga gtttgtggtc acggcaatac cggtcgaact
     4921 cggatgcgtc ttccccatgt ttgaagtagc cgatcatgcg cgtgacgtct ccccatccca
     4981 tccgccgcga ctcgagaatc gcggccacga cgtccatggt cagtgcgacc tgcttgcgca
     5041 aatcgcccac gtgacacgtc tcgccccccg gagcgatgct ggccgtgccg gaaacgtaga
     5101 ggcggcgttg gccgggcagc tcgagttcca ccgcccggct gaagctgctg ccatactcga
     5161 cggccggaca ctggagcggg gaagggatgg cctgaagcct gacggtcggg cccttcggtt
     5221 ggatggcaag gaggccggca atcacggccg tgccggcgga attgccgccc ccaatccccg
     5281 tgctcgccgg caccagacca tcgcatacct tgcgctcgag gaagaactgg ttgcgcaccc
     5341 cgttgaactc gtcgtaccaa tccagaatgt catccagata aaaccaggtc cgaaagacgt
     5401 tcgaaaaagt catccccgct aactcgagcg ccgactccat gatctcgaat gtttcccagg
     5461 cctgctcctc gcgcgtctcc tcgatcgact ccggatgcag cccgcccatg acgcagtagc
     5521 gcgcatgctg gtcttcgaaa agcgtgccga cggtatgtcc ttcgaggttg atggggtgaa
     5581 cgtcgatccc cgacacggcg tgcacttgaa ctccggcgat cgggccgcct ccgccgttgc
     5641 cctcctcgac ccacgtcatc ggccactcga tgcccgcgac acgatagccc ctggcatgcc
     5701 gaatcttgaa cggatcgatg tccagcccaa acacatcctc ccgcacgatc cggagttggg
     5761 cgttgtcggc cagaaaatcg tacaggcgtt gaaagagcga cgcaggttct tcggaaccga
     5821 ttgccggcac ggtgatgaac gtttcctgaa atccaccgtt cggcaggcgg atcacctgca
     5881 tgccatccac cgcttttctg gtcgaccgct tcttgtccat cgtgtctcgg gttccaacaa
     5941 gccgcctcgt gtgtcgagcg cggcagaaca ccccgattgc ccgatatttg accagggaag
     6001 tcaactgaac aatgccgccc ctgatcctcg cctaattttc tgaaagtgtg aaaaaagtgt
     6061 gtgtacatgc a
//
LOCUS       DAOQRO010000216         6071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13112_length_6071_cov_4.717753, whole genome shotgun sequence.
ACCESSION   DAOQRO010000216 DAOQRO010000000
VERSION     DAOQRO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13112_length_6071_cov_4.717753"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PKK20_08810"
     CDS             <1..1152
                     /locus_tag="PKK20_08810"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNV00024.1"
                     /translation="FDAAHTWVECADWVPAMLTGTEAPDKLTVGVCAAGHKAMYNDDW
                     DGYPDAEFLGQLEPKLGELRARLRPKAHTIDRAVGGLTPAWAARTGLPAGIPVAVGAF
                     DAHLGGVGSGIAPGTLVKIIGTSTCDMMVVPVGHSLADVPGLCGIVNGSILPGYFGLE
                     AGQSAVGDIFNWFVNYLQPGGAKLGSHEALSTAAAKLAPGESGLLALDWNNGNRTVLV
                     DQRLTGLLLGQTLYTTPAEIYRALIEATAFGALTIINRFEEYGVKVDQIVNCGGIAEK
                     NPLVMQIYADVTGRPMKVSRSGQTCALGAAIAGAVVGGAHPDYATAQKAMTGLKPRVY
                     KPKARNHQTYKALYALYRQLHDAFGTTSWTGNLHRVMKQLIEIRGQARQ"
     gene            1264..1965
                     /locus_tag="PKK20_08815"
     CDS             1264..1965
                     /locus_tag="PKK20_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNV00025.1"
                     /translation="MLDRLKEIVCRANVDLVDAGLVIQTWGNVSGIDRDRALVVIKPS
                     GVPYADMRPNDMVVMSLETGDVVEGKLKPSSDTPTHLVLYRAFRQIGGIVHTHSLYAT
                     AWAQARMPIPCWGTTQADDWHGEIPCTRLLKPSEIKTDYEANTGRVIVETFRHLDPRH
                     SPAVLVASHGPFAWGADARDAVHNAVVLEYVARLASQTLRLNPKVRPIAPALLDKHFL
                     RKHGPGAYYGQNSKR"
     gene            1986..3665
                     /locus_tag="PKK20_08820"
     CDS             1986..3665
                     /locus_tag="PKK20_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNV00026.1"
                     /translation="MHWLDWIVIALYFGLIGWLAWWYGRYQKDSVDYFLAGRNAGWII
                     IGASIFTSNIGSEHIVGLAGQGASTGMAMAHYEMHAWCLIVLAGLFVPFYYKSGVQTI
                     PEFLEKRFNARARWVLSCVSLVMYVFTKVSVTVYAGAIVFQALLPDTFGSPQNAFWIG
                     AFSTVIITGIYTVFGGMRAIMYTATPQAVIILVGSFIITGIGLAKLGGWGELVRQCKE
                     NADNFALWRPLSDPGFPWLGMLIASPIVGLWYWCTDQYIVQRTLSAKNLATARRGAMF
                     GGLLKVWPVLIFLIPGMIGWAMHQKGLIQLPMKLVDGTSTIDGDLVFPTLVKLLLPIG
                     IRGLIVACLLAALMSSLASLFNSSASLFTVDIYEKIRPGQSERHLLTVGRIATATVVG
                     LGIIWIPVMAKVSKGGLYQYLQNVQSYLAPSITAVFFLGLFWKRINATGAVWALVGGF
                     VLGMVKLTIQTFFGKGKIESPAFLAQIGDYNYLYATGLLFLASAIIMVVASLLTPPPP
                     PEKLKGLTYGSIHQVAGDEIKRSWDAGNKVLAATILLAVLSMYLYFSFWLR"
     gene            complement(3825..5057)
                     /locus_tag="PKK20_08825"
     CDS             complement(3825..5057)
                     /locus_tag="PKK20_08825"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNV00027.1"
                     /translation="MKSIYLLAAALAAGVSADATGQAASAKLGDPAPALEIAEWVKGG
                     PVDLAAGKGKKVFVVEFWATWCPPCRTSIPHLTELQKKYKADGVVFIGISDEKPGVVK
                     PFVEKQGNKMEYVVAIDKERKTSAGYMKAFGINGIPHAFVVDKEGRIVWQGHPMAELD
                     ETIRQILDGKYDMNKAKKRAEAQALLEEYYEVAADDSQKDRADELEKKLVAIDTEIGG
                     IVSGEKFDPREVRNQIRFSEAMREYGQAIMAGKDNASLAELEKKAEPFKPAGFEFGEY
                     REQLQASVVFREYMQLAQSEGEAEKLAEAATKLGGLKTKNPDILNQIAWTLLTDEKIK
                     QRDLGLASKLAKAAYDASEGKDASVVDTYARALFETGKVSEAIEHEKKAIELCDDEEM
                     KSDFVANLKKYQEKAAKK"
     gene            5170..>6071
                     /gene="mutL"
                     /locus_tag="PKK20_08830"
     CDS             5170..>6071
                     /gene="mutL"
                     /locus_tag="PKK20_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNV00028.1"
                     /translation="MNRIRLLPEQVANQIAAGEVVERPASVVKELVENALDAQAERIQ
                     VEVQAGGRSLIRVTDDGVGMSRDDALLCFDRHATSKIRRAEDLDAIATMGFRGEALPS
                     IASVSRLTLITRERTVESIEATQVLIHGGKIIGVPTASGPPGTSVEVRNLFFNLPARR
                     KFLKSEETERSHIHHYLLLAALAHPEVGFTFTHDRRLVWQLPPITSAQTASDRHAAVR
                     ERLRALYGTELNLVPVEASAPLAPSAPDSDSETDADSESPKDETRAPSAMRLWGFIGA
                     PGVSRATREDEHLFVNRRPVDNRTL"
BASE COUNT         1121 a         2091 c         1681 g         1178 t
ORIGIN      
        1 ttcgatgcag cgcacacctg ggtcgagtgc gccgactggg ttccagcgat gctcaccggg
       61 acggaggccc cggacaagct cacggtgggc gtctgcgccg ccggtcacaa ggccatgtac
      121 aacgacgatt gggacggtta ccccgacgcc gaattcctgg gccaactcga acccaaactg
      181 ggcgaactgc gcgcacggct ccgccccaaa gctcacacga tcgaccgggc ggtgggcggc
      241 ctcacgccag cctgggcggc gcgcaccggg ctgcccgcag gcatccccgt ggctgtcggc
      301 gccttcgacg cccatctggg cggcgtcggc tcgggcatcg ctccgggcac tctggtcaag
      361 atcatcggca ccagcacctg cgacatgatg gtcgtgcccg tcggccacag tctggcggac
      421 gtgcccgggc tctgcggcat cgtcaacggc agcatcctgc ccggctactt cgggctcgaa
      481 gcgggacagt cggccgtggg cgacatcttc aactggttcg tcaattatct ccaacccggc
      541 ggcgcgaagc tcggctcgca cgaggccctc tcgaccgcag ccgccaagct cgcgcccggt
      601 gaaagcggcc tgctcgcgct cgactggaat aacggcaacc gcacggtgct ggtcgatcag
      661 cgcctcacgg gactcttgct cggccaaacg ctttacacta cgccggctga aatctaccgc
      721 gccttgatcg aggcgacggc attcggcgcc ctcacaatca tcaatcggtt cgaggaatac
      781 ggggtcaaag tggaccagat cgtgaactgc ggcgggattg ccgagaagaa cccgctggtc
      841 atgcagattt acgcggacgt cacaggccgc ccgatgaagg tgagccgctc cggacaaacg
      901 tgcgccctgg gcgctgccat cgccggcgcc gtcgtcggcg gcgcccaccc ggattacgcc
      961 accgcccaga aggcgatgac cggtctcaaa ccgcgtgtct acaaacccaa ggccagaaac
     1021 caccagactt ataaggccct ctacgcgctc tatcgccaac tccacgacgc gttcggaacg
     1081 acgtcctgga cgggaaacct ccatcgcgtg atgaaacaac tgatcgaaat acgcggccag
     1141 gcgcggcagt aattgtccgg accacagtca ttgttgctga ccaccgaaca cgcggaagac
     1201 acgaaagagg cgtaaaagcc acggcattga actgccgtac gaggaagaga cttacaggat
     1261 tatatgctcg atcgactgaa agaaatcgtt tgccgcgcca atgtcgattt agtggacgcc
     1321 ggcctcgtga tccagacctg gggcaacgtg agcggcattg atcgcgaccg cgccctggtc
     1381 gtcatcaagc catccggtgt gccgtacgcc gacatgcggc caaacgacat ggtggtgatg
     1441 tctctcgaga ccggcgacgt cgtcgagggc aaactcaagc ccagctccga caccccaacg
     1501 cacctggtcc tgtaccgcgc gttccggcag atcggcggga tcgtccacac ccacagtctc
     1561 tacgccaccg cctgggccca ggcgcgcatg ccgattccct gctggggcac cacgcaggcg
     1621 gatgattggc atggagagat cccctgcaca cgactgctca aaccgtcgga aatcaaaacc
     1681 gactacgaag ccaacactgg acgcgtcatc gtcgaaacct tccgtcacct ggacccccgc
     1741 cactctccgg ctgtactggt ggccagccat ggcccgttcg cgtggggcgc cgacgcacgg
     1801 gatgcagtcc acaacgccgt ggtcctcgag tacgtcgccc gcctcgccag ccagacgctg
     1861 cgcctgaatc cgaaggtccg cccgattgcg cccgccctgc tcgacaaaca ttttctccgg
     1921 aaacatggcc ccggagcgta ctacggtcag aactcgaaac gctaacccaa gaaagaaccc
     1981 gcaacatgca ttggctcgac tggatcgtga tcgcactcta cttcggtctg attggctggc
     2041 tcgcctggtg gtatggacgc tatcaaaagg acagcgtgga ttacttcctc gcaggccgca
     2101 acgccggctg gattatcatc ggtgcctcga tcttcacctc gaacattggg tccgagcaca
     2161 ttgttggact ggccggccag ggggcttcga ccggcatggc gatggcgcat tatgaaatgc
     2221 acgcctggtg tctgatcgtg ctggcggggc tctttgtacc tttctactac aaatccggcg
     2281 tgcagacgat tcccgagttt ctcgagaagc gcttcaatgc ccgggcccgc tgggtgctct
     2341 cgtgcgtttc cctggtcatg tacgtgttca ccaaggtcag cgtcaccgtc tacgccggag
     2401 ccatcgtctt tcaagccctg ctcccggaca ccttcggctc cccgcagaac gccttctgga
     2461 tcggcgcctt ctcgacggtc atcatcaccg gcatctacac ggtgttcggc ggcatgcgcg
     2521 ccatcatgta taccgccaca ccccaagccg tcatcatcct ggtcggttcg ttcatcatca
     2581 ccggcattgg cctggccaaa ctgggcggct ggggcgaact ggtgcgccag tgcaaggaga
     2641 atgccgacaa cttcgccctc tggcgcccgt tgtccgatcc cggtttcccc tggctcggca
     2701 tgctgatcgc ctcacccatc gtcggcctct ggtactggtg caccgaccag tacatcgtcc
     2761 agcgtactct gtcggcaaag aatttagcaa ccgcccgccg cggcgccatg ttcggcggat
     2821 tgctcaaggt ctggccggtg ctgatcttcc tgatcccggg tatgattggc tgggccatgc
     2881 accagaaggg cctcatccaa ctccccatga agctggtcga cggcacctcg acaatcgacg
     2941 gcgacctggt cttccccacc ctggtcaaac tgctcctccc gatcggcatc cgcggtctga
     3001 tcgtcgcctg tctgctcgcg gcgctgatga gttcgctggc ctcccttttc aattcgagcg
     3061 cctccctgtt cacggtcgat atctatgaga aaatccgacc cggccaatcg gaacgtcacc
     3121 tcctgaccgt cggacgcatc gccaccgcca ccgtcgtggg gttaggcatc atctggattc
     3181 ctgtcatggc caaggtctcg aagggcggac tctaccaata cctccagaat gtccagagtt
     3241 acctggcccc atcgatcaca gccgtcttct tcctcggcct gttttggaaa cgcatcaatg
     3301 ctacaggcgc cgtttgggcc ctcgtcggcg gcttcgtcct cggcatggtc aagctgacca
     3361 tccagacctt cttcggcaag ggcaagatcg agtcgcccgc ctttctggcc cagatcggcg
     3421 actacaacta cctctacgca accggcctgc tcttcctggc cagcgcgatc atcatggttg
     3481 tcgcctccct gctgactccg ccgccgccgc ccgagaaact caaaggcctg acctacggct
     3541 cgatccacca ggtggccgga gacgaaatca agcgaagctg ggatgcgggc aacaaggtcc
     3601 tggccgccac catcctgctg gccgtgctct cgatgtacct ctacttcagc ttctggctgc
     3661 gctaagaagc ccggagcaac gaaaaccagg agcgctccgg cggccgatgc ctcccgagcg
     3721 ccagctcttc gccattatga aaacgccctc gagggcatgc gctcgagggc gctgatttga
     3781 cgctgatcca acctcccctt caggggaccg ttctgccagg gcgcttactt cttggccgcc
     3841 ttctcctgat acttcttcag gttggcgaca aagtcgctct tcatctcctc atcgtcgcac
     3901 aactcgatcg ccttcttctc gtgctcgatg gcttcgctga ccttgccggt ctcgaacagc
     3961 gcccgggcat aggtgtccac aaccgatgcg tccttgccct cggaggcgtc gtaggccgcc
     4021 ttggcaagct tcgatgccag cccgaggtcc cgttgcttga tcttctcgtc cgtcagcagc
     4081 gtccaggcaa tctggttaag gatgtccgga ttcttggtct tcagcccgcc cagtttggtg
     4141 gcggcctcag ccagcttctc cgcctcccct tcgctctgcg caagctgcat gtactcccga
     4201 aaaaccacag aagcctgcaa ctgttcacga tactcgccaa actcgaatcc cgcaggcttg
     4261 aacggctcgg ccttcttctc gagttccgcc agggaagcgt tgtctttgcc cgccatgatc
     4321 gcttgaccgt actcgcgcat cgcctccgag aaacgaatct ggttccggac ctcccgcgga
     4381 tcgaacttct caccggacac aatgccgccg atctcggtgt cgatggccac gagtttcttc
     4441 tcgagttcat cggcccggtc cttctggctg tcgtccgccg ccacttcgta atactcttcg
     4501 agcaacgcct gggcctcggc gcgtttcttg gccttgttca tgtcgtactt gccatcgagg
     4561 atttgccgga tcgtttcgtc caattcagcc atcggatgcc cctgccacac aatccgcccc
     4621 tccttgtcga ccacgaatgc atgcggaatc ccgttgatcc cgaaagcctt catgtacccc
     4681 gccgaggtct tccgctcctt gtcgatcgcc accacgtact ccatcttgtt cccctgcttc
     4741 tcgacaaacg gcttcacaac ccccggcttc tcgtccgaaa tgccgataaa caccaccccg
     4801 tccgccttgt acttcttctg cagctccgtc agatgtggaa tgctcgtccg acacggagga
     4861 caccacgtcg cccagaactc tacgacaaag accttcttcc ccttcccggc agcaaggtcg
     4921 accggacctc ccttcaccca ttctgcgatt tcgagcgcgg gagcggggtc gcccagcttc
     4981 gcgctcgccg cttgcccggt tgcgtcagcc gacacaccag ccgccaaagc ggccgccaat
     5041 aaataaattg atttcatgtt taagatttgc tctgcgcggc ggaacgtgct gcataaatgg
     5101 cgcgccgtca accacggctt tgaatgatgt atggcacccc ccggatcggg agggtagtct
     5161 gtccgctgca tgaaccgcat ccgcctgttg cccgagcagg tggcgaacca gatcgccgcc
     5221 ggcgaggtcg tcgaacgccc ggccagcgtg gtcaaggaac tggtcgagaa cgccctcgac
     5281 gcccaggcgg aacgcatcca ggtcgaagtc caggccggcg ggcgaagcct gatccgagtc
     5341 accgacgacg gcgtcggcat gagccgcgac gatgccttgc tctgtttcga tcgtcatgcg
     5401 acaagcaaaa tccggcgcgc ggaggatttg gacgcgattg ccacgatggg gtttcgcggt
     5461 gaggcgctcc cgagcattgc cagcgtcagc cggctgacct tgatcacgcg cgagcgaacc
     5521 gtcgaatcga tcgaggccac ccaagtcctc attcatgggg gcaagatcat cggcgtgccc
     5581 acggccagcg ggccgcccgg cacctcggtc gaggtgcgca acctcttttt taacctgccg
     5641 gcccgacgca agttcttgaa gagcgaggag acggagaggt cccatatcca tcactacctc
     5701 ctcctggcag ccctcgcgca tccggaagtc ggtttcacct tcacccatga tcgacgcttg
     5761 gtctggcagt tgcctccaat cacttcagcc caaaccgcgt ccgaccgtca cgccgccgtc
     5821 cgcgaacgct tgcgtgcctt gtatggcacc gagttgaacc ttgtccccgt cgaggcctct
     5881 gctcctctcg ccccgtccgc accggacagc gactcggaga cggatgccga cagcgaatcc
     5941 ccgaaagatg aaaccagggc gccctccgcc atgcgcctct ggggcttcat cggcgcgccg
     6001 ggagtctccc gggcaacacg cgaagatgaa cacctgttcg tgaaccggcg ccccgttgac
     6061 aatcgcaccc t
//
LOCUS       DAOQRO010000217         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13156_length_6053_cov_5.213571, whole genome shotgun sequence.
ACCESSION   DAOQRO010000217 DAOQRO010000000
VERSION     DAOQRO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_6053_cov_5.213571"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1079
                     /locus_tag="PKK20_08835"
     CDS             270..1079
                     /locus_tag="PKK20_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00029.1"
                     /translation="MNGWSHRGWGCALALAVSSALAAEAPTNALPPLDTVLHGIVQRA
                     EADARIQQEFLARYAFVHTVTRQEHNPKGKLKKRKTKTVSHDPSVQNSTPPPSRSSDG
                     GQNRAHRSHARPEAVANEEPLDRPYEREDVVVTDGLMRRFEFTLLGRELWETLPLLKL
                     DFRPAVPTLHAKGVLERLINSMAGTLWVDERDYTVARISLRLIDKVNVGVGGLLGSVS
                     AFQCEFERSRTCDGLWYTRRVDWRAEYREFLVNKIVEQQETRTEVRRVTEK"
     gene            complement(1237..2232)
                     /locus_tag="PKK20_08840"
     CDS             complement(1237..2232)
                     /locus_tag="PKK20_08840"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNV00030.1"
                     /translation="MFEQCTIRGVQFVSARFLAPMAGYTHSAFRRLLGELGGCGALWT
                     EMLDARQVIAEDFRKSPWVKRRPSDGFTFYQLMARAGDPLDRVLGLLAAQGVEAVDLN
                     LACDAFSIRACEAGSSLFEDLAALARVARESRRHWPGLLTAKVRLGRQRPDWEIRFAE
                     RLRLLEDAGFDALVVHPRFFEDKFRRRARLELLPWVATQTRLPLIANGDLAGVESVAA
                     HLPCLESASGIMIGRMAIVCPWLFACWDGAPREVDHAAVWGRLCDYVAEDFPAAAALR
                     RVQMFTKYFAANFAFGHRFRVEIANAPNLEQARERAHEFFARSPQMLAHRTVAGL"
     gene            complement(2312..3451)
                     /locus_tag="PKK20_08845"
     CDS             complement(2312..3451)
                     /locus_tag="PKK20_08845"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV00031.1"
                     /translation="MRKTIRLEGPVTARNRHTSRAGPEFRRRRESHYHTEILATHTPA
                     LFAAAVDRASALLRAGEIVALPTETVYGLAANAYDKKAVARLFAAKGRPAHNPIIVHV
                     ANVAMARRCVASWPRAAQRLARAFWPGPLTLVLPKATTIPHIVTSGGATVGVRCPRHS
                     FIRSVILECGFPLAAPSANRSTALSPTRAEHVRASLGGRIPLIVDGGPARVGIESTVV
                     DLTARPLRVLRSGMIHRDALTAVLGVSGGEIRIDQTEGGEPVAGAPRRSPGQLARHYA
                     PRTPLALLEWNDEADLRRQVRRLGWARRAVQILAHTNIPGREGWGGMGVIPRDPEGCA
                     QALYAELHRCDQRGGAGIVIEAVPEDPRWEGIADRLRRASASRYT"
     gene            complement(3598..4695)
                     /locus_tag="PKK20_08850"
     CDS             complement(3598..4695)
                     /locus_tag="PKK20_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNV00032.1"
                     /translation="MNSSSFLNRCVSAGLAAGVLVGLPVMDQQALAAGDPAIVHSEVI
                     APNPPFASSHASTIVETPDGLVAAWFGGSDEGAPDVSIWLSRNEGGTGWSAPEEIANG
                     TDTRQFRRYPCWNPVLFLRKNGQLLLFYKVGPSPSTWWGMVRMSTDNGKTWVKAGRLP
                     AGYIGPVRNKPIEMADGTLLCGASWEDKGWRVRMEWAKDPFGIWSCGPYLNAAFTMAV
                     IQPTILRHGSGTYQLLCRSKQGRIMTCWSQDNGQSWSPMDRTPLPNPNSAIDGVTLDD
                     GRQLLIYNHTEKGRGVLNAAISTDGKIWEAVCELENEPDSEFSYPAAILSKNGFVHVT
                     YTWKRERIKHVVLDPSRFKGRVMVDGRWPAP"
     gene            complement(4837..>6053)
                     /gene="rsmB"
                     /locus_tag="PKK20_08855"
     CDS             complement(4837..>6053)
                     /gene="rsmB"
                     /locus_tag="PKK20_08855"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNV00033.1"
                     /translation="LLHSTLERASLHPRDRALVHELVCGVVRWQATLDWLIDQKTRGR
                     RQKPDLRVLLHLGLYQIFWLDRIPDHAAVNETVNLARQHGLSAQAGFVNALLRGYARE
                     RESTRLQLEVLKRAQPAVGWSHPAWLIDRWQARWGFEPTVALLAWNNAPAYTFARANA
                     LKTDPGRLLDRWRQEDVEYDFGRWDWIPESLVFQLKTHPPLARLGTFQDGWFYLQDPS
                     TLLAVHALDPQPGDVVLDACAAPGGKTMYIAQRMANQGRIVAQDASAARLGLLHDNCR
                     RLGITCVQTCLTTDPLSADFDRILVDAPCSNTGVMRRRIDLRWRIRSEAIPRLKAEQC
                     DVLDRVAARLKPGGRLVYSTCSLEPEENQQVARALLSRRSELRLQTERELLPFRDRVD
                     GAYVAVFDRALG"
BASE COUNT         1116 a         1923 c         1982 g         1032 t
ORIGIN      
        1 gaaaagagac atccgatctg tggtctccgt cgctaccttt gtcgctgacg cgccggcagt
       61 cgacaaaggt ggcgacaaag gcggtgacgc tgcaaaattt gttgacacgc gaggcggtgg
      121 tcacgactcc atgcaacatg ctttctagcc cgcatagcag tcatcttgcg aagtgtgagt
      181 gctctgtgca ccataggggt gcgaaacatg cgggctggca cgaaacgctt tccagcaccg
      241 gcgccctgtg caatgtatgg ggccgatcga tgaacggttg gagtcataga ggctggggat
      301 gcgccctcgc cttggccgtg agcagcgcgc tcgccgccga ggcgcccaca aatgccttgc
      361 cacccctcga caccgtgctg cacggcatcg tgcaaagggc cgaggccgat gccaggatcc
      421 agcaggagtt cctcgctcgc tatgccttcg tccacaccgt gacgcgccag gagcacaatc
      481 ccaaaggcaa actcaagaag cgcaaaacca aaaccgtgtc tcacgatccc tcggtgcaga
      541 actcgacccc gccgccttcg cgttcctcgg acggcggcca gaaccgggcc catcgcagcc
      601 atgcgcggcc cgaggccgtc gccaatgagg aaccgctgga ccgcccctac gagcgggagg
      661 atgtggtcgt gaccgacggt ttgatgcggc ggttcgagtt cacgctcctc ggacgcgaac
      721 tatgggaaac cttgccgctc ttgaagctcg attttcggcc tgcggttccg actctccacg
      781 ccaagggagt cctcgagcgg ttgatcaaca gcatggccgg aaccctctgg gtagatgaac
      841 gggattacac tgtcgcgcgc atcagcctcc gcctgatcga caaggtcaat gtcggcgtgg
      901 gcggcttgct cggctcggtc tcggccttcc agtgcgagtt cgagcgatct aggacgtgcg
      961 acggactctg gtatacgcgc cgggtcgatt ggcgcgccga gtaccgcgaa ttcctcgtca
     1021 acaagatcgt cgagcagcag gaaacccgaa ccgaggtgcg ccgggtcacc gaaaagtagc
     1081 agtggggtca catcttcata tttgacaatt gtctgcccgt tgcgcgaatt cggtcatggc
     1141 gggtctgccc cgtgaagtcc cccgggcgag gcggcctgat gccttggaac attctcagca
     1201 caatgtcaaa tatgaagatg tgaccccaag ggcgggttag agcccggcga ccgtgcgatg
     1261 cgcaagcatc tgcggggatc gggcaaagaa ctcatgggcc cgttcacggg cctgttcgag
     1321 attcggcgcg ttggcgatct cgacccggaa tcggtggccg aaggcgaagt tggccgcaaa
     1381 gtacttcgtg aacatctgga cccggcgcag ggccgccgcc gcgggaaaat cctcggccac
     1441 gtaatcgcac agcctgcccc acacggccgc atggtcgacc tctcggggtg cgccatccca
     1501 gcaggcgaac agccacgggc agacaatcgc catgcgaccg atcatgatcc cggacgcgga
     1561 ctcgaggcag ggcaagtgcg cggcaacgga ctcgaccccg gccaggtcgc cgttggcgat
     1621 caagggcagg cgcgtctgtg tggccaccca cggcagcaat tcgagccgcg cgcgccggcg
     1681 gaacttgtct tcgaagaacc tcggatggac gaccaatgcg tcgaatccag cgtcttcgag
     1741 cagacgcagg cgttcggcaa agcggatttc ccaatcggga cgctggcggc ccaaccggac
     1801 cttggctgta agcagaccgg gccagtgtcg acgagactcg cgcgccaccc gggccagggc
     1861 ggcgaggtct tcgaaaaggc tgctgccggc ttcgcaggcg cggatcgaga aggcgtcaca
     1921 ggcgaggttc aagtccacag cttccacccc ctgcgccgcc agcagaccca gcacccggtc
     1981 cagagggtcg cctgcccgcg ccatgagttg gtagaaagtg aacccgtccg aggggcgccg
     2041 cttgacccat ggagacttcc ggaagtcttc ggcgatgacc tggcgcgcat ccagcatctc
     2101 cgtccagagc gcaccgcaac cccccaactc gcccagcaat cggcgaaagg cgctgtgggt
     2161 gtaaccggcc atgggcgcca ggaaacgcgc cgacacgaat tgcacgccgc ggatggtgca
     2221 ttgctcgaac attgcgcatc ctcgcgcatc ccgcggtcac cggtgctcga gccgacgaca
     2281 cgccggcgct ccggtcgcgg gacttctcgc gtcatgtgta acgagaggcc gacgcgcgac
     2341 gcaggcggtc ggcgatcccc tcccaccgcg ggtcctccgg caccgcctcg atgacgattc
     2401 ccgccccgcc ccgctggtcg catcggtgga gttccgcgta gagcgcctgg gcgcagccct
     2461 cggggtcgcg gggaatcacg cccatgcccc cccagccctc gcgcccggga atgttcgtgt
     2521 gggcaagaat ctggacggcg cgccgggccc agccgagccg cctgacctgt cggcgcaggt
     2581 ctgcctcatc gttccactcg agcagggcga gcggcgtgcg cggcgcgtaa tgccgcgcca
     2641 gttgccccgg gctgcgacgc ggcgcgcccg cgaccggttc tccgccctcg gtttgatcga
     2701 tccgaatttc acctccggac acccccagga ccgccgtcaa ggcgtcgcga tggatcattc
     2761 ccgaccgcag cacacgaagt ggcctcgcag tcagatcgac caccgtcgac tcgattccca
     2821 cccgagccgg cccgccgtcc acaatcagcg ggattcggcc tcccaggctc gcgcggacgt
     2881 gctcggcacg agtcggcgaa agcgcggtcg accgatttgc gctgggagcc gccagtggaa
     2941 aaccgcactc gaggatcacg gaccggatga aggagtgccg aggacaacga acgccaacgg
     3001 tcgcgccccc gctcgtgaca atatggggaa tcgtggtggc cttgggcaga acgagcgtca
     3061 gaggtcccgg ccagaatgcc cgggccaggc gctgggccgc gcgcggccag cttgccacac
     3121 accgccgcgc catggccaca ttagccacat gcacgatgat gggattgtgc gccgggcggc
     3181 ccttggccgc aaagagacgg gccacggcct ttttgtcgta tgcgttggca gccaggccgt
     3241 aaacggtctc ggtcggcaag gcgacgatct cgccggcccg cagcagggcg gaagcgcgat
     3301 cgaccgcagc ggcgaacaac gcgggggtgt gggttgcgag aatctcggtg tggtaatgag
     3361 attctcgacg cctgcggaat tccggacctg ccctcgacgt gtggcggttt cgggccgtaa
     3421 caggcccctc gagccgaatt gtcttgcgca agtctctggg cggtctggtc ttcgggctga
     3481 accgcatggt tcatggagtt gtgaccaccg caaagccgga aactcaaaga tcgagaccca
     3541 cgcgggctgg aaaaagcccg agcccctgca tcctcgtccc ggcatcagtc tccaaggcta
     3601 aggtgcgggc caacggccat ccaccatgac gcgtcccttg aaccgcgatg ggtcaagcac
     3661 gacatgcttg atgcgctcgc gcttccatgt gtaggtcacg tggacgaagc cgtttttgct
     3721 caggatggcg gccgggtacg agaactcgct gtccggctcg ttttcgagtt cgcacacggc
     3781 ctcccaaatc ttgccatcgg tcgagatggc ggcattcagg acgccccggc ctttttcagt
     3841 gtgattgtag atcaagagct ggcgcccgtc atcgagcgtc actccgtcaa tcgcgctgtt
     3901 tgggttcggc agcggagtgc ggtccatcgg actccaggat tggccgttgt cctgggacca
     3961 acaggtcatg atccgcccct gtttcgatcg gcacagcaat tggtaggtgc cggatccatg
     4021 ccgcagaatg gtcggttgaa tcacggccat cgtgaacgcc gcattcagat acggcccgca
     4081 ggaccagatg ccaaaaggat ccttggccca ctccatgcgg acccgccatc ctttgtcctc
     4141 ccaactggcc ccgcacagca atgtgccgtc cgccatctcg atcggtttgt tccgaaccgg
     4201 gccgatgtag cccgcgggca agcggcccgc tttgacccag gttttgccgt tgtcggtgga
     4261 catgcggacc atgccccacc aagtggaggg gctggggccg accttgtaaa agagcagcaa
     4321 ctggccgttc ttgcgcagga acaggacagg attccagcac gggtagcggc ggaactggcg
     4381 cgtgtcggtg ccgttggcga tttcctcggg tgccgaccat cccgtcccgc cttcgttgcg
     4441 ggagagccag atgctgacgt cgggcgcccc ctcgtcgctc ccgccgaacc acgctgccac
     4501 caggccatcg ggtgtctcga caattgtcga ggcgtgactg ctggcaaagg gcggattcgg
     4561 cgcaatgacc tccgaatgga caatcgcagg atcccccgcg gcaagcgcct gctgatccat
     4621 gaccggcagt ccgaccagga ctcccgcggc caatccggct gagacgcatc gattgagaaa
     4681 tgaagacgag ttcatgtcat gacaaattat cggccaccga ctgccgtcta ttgttagacg
     4741 cgacgcgaga ctttttcaat gcttaatcat gaaatgcggg ttagcgaatc cggttcagcg
     4801 cggcgcctgt gagcgatccg gaccatgtgt tgccgactat ccgagcgcac gatcaaagac
     4861 ggccacatag gcgccgtcga cacgatcgcg aaacgggagg agttcgcgct cggtctggag
     4921 acggagttcg gaacggcggc tcaggagggc acgggcaacc tgttgattct cctcgggttc
     4981 gaggctgcaa gtgctgtaga cgaggcggcc tcccggtttc aatcgggctg ccacgcggtc
     5041 cagcacatcg cattgctccg ccttcaagcg cgggatcgcc tcagaccgga tgcgccaacg
     5101 gagatcgatg cgtcgccgca tgacgccggt gttggagcag ggggcatcga ccaggatgcg
     5161 atcgaaatcg gcggacagcg gatcggttgt caggcaagtc tggacgcatg tgatccccag
     5221 tcgcctgcag ttgtcgtgca ggagcccgag ccgagcggcg gaggcgtcct gggctacgat
     5281 ccggccctga tttgccatgc gttgcgcgat gtacatggtc ttgcctccgg gcgcggcgca
     5341 ggcgtccaga acgacatcgc cgggctgggg atcgagggca tggacagcca gcagggtgct
     5401 cgggtcctgg agatagaacc aaccgtcctg gaatgtgccc agcctggcga ggggcggatg
     5461 ggtcttgagc tggaagacca ggctctcggg aatccaatcc cagcgtccga aatcgtactc
     5521 gacatcctcc tgtcgccatc gatccagcag gcggcccgga tcggtcttga gagcattcgc
     5581 gcgcgcaaag gtgtaggctg gggcgttgtt ccacgccagc agcgcaaccg tcggctcgaa
     5641 gccccaccgt gcctgccatc ggtcgatcag ccaggcggga tgggaccatc cgactgcggg
     5701 ttgggctcgt ttcagcacct cgagctggag ccgtgtcgac tccctctcgc gcgcgtagcc
     5761 gcggaggagg gcgttgacga agccggcttg ggcgctgagg ccgtgttggc gggcgaggtt
     5821 gacggtttca ttgaccgcgg cgtggtcggg gatgcggtcg agccagaaga tttggtagag
     5881 gccgaggtgg aggagcaccc ggagatcagg tttttgcctg cgtccccggg tcttctgatc
     5941 gatgagccag tcgagggtcg cctgccagcg gacgacgccg cagaccagtt catggacgag
     6001 cgcgcgatcg cgcgggtgga gagaggcgcg ttcaagggtg ctgtggagca ggt
//
LOCUS       DAOQRO010000218         6014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13307_length_6014_cov_5.315321, whole genome shotgun sequence.
ACCESSION   DAOQRO010000218 DAOQRO010000000
VERSION     DAOQRO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13307_length_6014_cov_5.315321"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="serA"
                     /locus_tag="PKK20_08860"
     CDS             complement(<1..970)
                     /gene="serA"
                     /locus_tag="PKK20_08860"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNV00034.1"
                     /translation="MKVIICDPVSPKGVALLQQRAEFKVQVLEKRLPEAELIPVVADA
                     VALVVRSETKVTQAVLEAAPRLKVVGRAGVGVDNVDVEAATQRGVVVMNTPGGNTIST
                     AELTFSMLMALARRIPQAHMSMASGQWNRKSFQGTELYTKTLGILGLGRIGSEVARRA
                     IAFGMRVIAYDPYLTLSRAMALQVEVVENVFDLYPQVDFLTVHMPMSDETRGMVDAEA
                     FARMKRGVRVLNCARGGIINEADLRVALESGQVAGAALDVYETEPLPKDSPLRTLPQV
                     ILTPHLGASTDEAQDNVGIEVAEAVTEYLLHGTVRNAVNLPSLDAAT"
     gene            complement(1489..2190)
                     /gene="tmk"
                     /locus_tag="PKK20_08865"
     CDS             complement(1489..2190)
                     /gene="tmk"
                     /locus_tag="PKK20_08865"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNV00035.1"
                     /translation="MKRTIPGGFLLAIEGIDGAGKSVQAKAVAAVLLERGLDVVLTRE
                     PTLGPWGQLLRESAAKGRLSPADELKAFIEDRKQHVAELIRPSLDAGRIVITDRYYFS
                     TVAYQGARGFNPEDLLRRNEAIAIEPHLLILLDIAPTVGLARIGLRDSAANEFETLAT
                     LTRSRDIYLSIRKPYAVRLDGTAPPDEIRDRILFTLGRAVLERLHLETGLTPRQRLDA
                     SLALHGSDGMAGECA"
     gene            complement(2187..2804)
                     /locus_tag="PKK20_08870"
     CDS             complement(2187..2804)
                     /locus_tag="PKK20_08870"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNV00036.1"
                     /translation="MFTGIVEETGVVVDIRPADKAIELTVRPGLCARGMRVGDSLAVN
                     GCCLTVVKLAGRGARASARFDLLRETWQRTNLQFLRPGSLVNLERSLRADSRLGGHFV
                     TGHIDGVGRIRTWERVGQDWVLDVQAPPEVMRYLVYKGSIALDGISLTVAAVRKREFR
                     VWIIPHTFEVTNLREKKVGDALNLEADLVGKYVEQMLGKMDLRRR"
     gene            3189..>6014
                     /locus_tag="PKK20_08875"
     CDS             3189..>6014
                     /locus_tag="PKK20_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00037.1"
                     /translation="MTDTNAGSDQPKQPRRLVRWAGGLGVALVLLVVALYFLAISEPF
                     VKGVVLPKAGAALGVDIAADGVSIRPLSEVILRGLKVRPQGEETVLEAAELRARYNLV
                     SILRGLIKVDEIALIQPVVRVTMRPDGTSNLDAFTGRSGTPEAASAKPGSPLKVDVRM
                     ISIDDATVHFTQTHKDGSATSAELAGLDAKASGIRNGSPAKLDLDGRIQLVQRTGTNQ
                     ADVAGTLRSAFGIDLDSDLAPAQITGSADFAVTRATGAFADASELQGRLSADFNLSEI
                     KQLAIAFTKGAAALGTASVSGPFDPLEQEGKLKVKVTGIGSELLSLVGGRFGISFGGT
                     KLAADYDIELKQNARFVSTTGSITATSFSVERAGLRTPTVDFKLGCDATLDLPRTNAT
                     IRAFTLDATQSGRPLLTGKLTKEMLLDWSQGAQEAAESALRLNVTELNLADWKPFVGD
                     SVDSGIVNGQLDINVQKAGRLVAFDLGSKLVGLTGRLGSNRLDQADLAVSLRGQLTEF
                     DRVDFAALALDMFQRGRAVSTLKGSGSYVNSTSDANFQVELTASLAALSELLGRTDLS
                     LTAGTATFTGKVSQKNLSPAQTAHPSLDQSLAGKLEIVGLIGILASNRLDRFDISSDV
                     DASLKNNLAEIRKCVGSIRQADNPAGTFNLTGRYGLTNGSGQIALEFLGLNQNALQPF
                     VTEALGDKTLASVTVNGQIAGGYDPAGDSRIRADVQITNLVVNIPSSAPASTPLEAQF
                     TLDTAVRTNVAEIREMLLTLTPTSRAANRIQATGRVDFTQTNAATGHLAITADALDAT
                     RCYEILFEETAPSASGESAGGRPGSSGAADQELEAMTLPLRNFIVDANVRRFYLREVE
                     ITNLVARARLDGQTIEVKPFDLALNGGSVSATAIVDLGVPGYRYDVGFKGTNVPVAPL
                     ARRYRREGTGPGSGTGAGFAQCQGAGTT"
BASE COUNT         1107 a         1969 c         1914 g         1024 t
ORIGIN      
        1 aggtggccgc gtccagactg ggcagattca ccgcgttgcg caccgtgccg tggaggagat
       61 actccgtcac ggcctccgca acctcgatgc cgacattgtc ctgggcctcg tcggtgctcg
      121 cgcccaagtg gggcgtcagg atcacttgcg gcagggtccg cagcggagag tccttgggca
      181 gaggctcggt ctcgtacacg tccaaggccg cgccagccac ctggccgctt tcgagcgcaa
      241 cccgcagatc ggcctcattg atgatgccgc cccgggcgca attgaggacg cgcacgccgc
      301 gtttcatgcg ggcgaacgcc tcggcatcga ccatcccgcg cgtctcgtca ctcatcggca
      361 tatgcacggt gagaaaatcg acctgcggat agagatcgaa gacgttctcg accacctcga
      421 cctggagcgc catggcgcgg gacagcgtga ggtaggggtc ataggcgatg acgcgcatgc
      481 cgaaagcgat tgcgcggcgg gcgacttcgc tgccgatgcg gcccaggccg aggatgccca
      541 gcgttttcgt gtacagttcg gtgccctgaa agctcttgcg gttccattgg cccgaggcca
      601 tggacatgtg cgcctgcgga atgcgccgtg ccagagccat cagcatggaa aaggtcagtt
      661 cggcggtcga gatagtgttt ccacccggtg tgttcatcac caccacgccc cgctgcgtgg
      721 ccgcctcgac atccacattg tccacgccga ctccggcgcg gcccacaact ttgagcctgg
      781 gcgccgcctc gaggacagcc tgcgtcacct tggtttccga gcggaccacc agggcgaccg
      841 cgtccgccac gacggggatc aactcggctt cagggagccg cttctcgagc acctggacct
      901 tgaactcggc gcgttgttgg agcaaggcca cccctttcgg tgacaccgga tcgcagataa
      961 taaccttcat tttgactgag tcttttatgc cgcacgcaac aaggcgcgca ctctaccaaa
     1021 cgaccctcat tggtcaaatc ctctgtccga ttcgccgccc atcgccagcg ccaattcatg
     1081 aagccgcaaa ggccggagcg gggaaggctt gtccaccgcc aacgcgagtg aagacggtat
     1141 ttgcaggaga cggggccctg gcccaccgcc gccccacgcc cagctagccc gcatattcca
     1201 cacctcgatg gcgcacaggg cactcagact tcgcaagagg actgctatgc gggctcgaga
     1261 agccgagaag caaaaccgcg ccaggttcga gcgatcgaga actgcatcgc actgttctca
     1321 tgagagacca ttctcaggat cgatagaatg ctcccgattc cctttccggg cgccgttttg
     1381 ccagagcccg gatgtcagac tccgcgaagc tttgtgaaac atccgggcta gcgcgcattt
     1441 ctcaccgact tctgaagaac gcggagtcga tgtcatcgga acgtgcactc atgcgcactc
     1501 gcccgccatc ccgtcgcttc cgtgcagggc gaggctcgca tcgagcctct ggcggggtgt
     1561 caggccggtc tccaaatgca gccgttccaa cacggcccga ccgagcgtga aaagaatccg
     1621 atcgcgaatc tcatcaggcg gcgcggtgcc gtcgagccgg accgcatacg gtttgcggat
     1681 cgagagatag atgtcgcgcg atcgagtgag tgtggcgagg gtctcgaatt cgttggccgc
     1741 gctatcccga aggccgatgc gggccaggcc gaccgtcggg gcgatatcga gcaagatcag
     1801 gagatgcggc tcgatggcga tggcctcatt gcggcggagg aggtcctcgg ggttgaatcc
     1861 ccgggccccc tgataggcga ctgtcgagaa atagtaacgg tcggtgatca cgatcctgcc
     1921 ggcgtcgagg ctggggcgga tcagctcggc cacgtgctgc ttgcggtcct cgatgaacgc
     1981 cttcaactcg tcggcgggcg acaggcggcc cttggccgcc gactcgcgca gcaattggcc
     2041 ccacggcccg agcgtgggct cgcgtgtcag aacgacatcg agcccccgct ccaggagcac
     2101 ggcggcaacg gccttggcct ggacgctctt gccggcgccg tcgatgccct cgatcgccag
     2161 aagaaaaccg ccggggatcg ttcgcttcat cgccggcgca ggtccatctt gccgagcatc
     2221 tgctcgacgt atttccccac gaggtcggct tcgagattga gggcgtctcc gaccttcttt
     2281 tcgcgcaggt tggtgacttc gaacgtgtgg gggatgatcc agacgcgaaa ctcgcgcttg
     2341 cgcacggccg ccacggtcag actgatgccg tccagcgcga tcgacccctt gtacaccaga
     2401 taccgcatca cctcgggcgg cgcctggaca tcaagcaccc agtcctggcc gacccgctcc
     2461 cacgtccgaa tccgccccac gccgtcgata tgccccgtca cgaaatgccc gcccagccgg
     2521 ctgtcggcgc gcagcgaccg ctcgaggtta accagggagc ccgggcgcag gaactgcagg
     2581 ttggtgcgct gccaggtctc ccgcagcagg tcgaaccgcg cggatgcgcg agcgccgcgc
     2641 ccggccagct tgaccaccgt caggcaacac ccgttgaccg ccagactgtc gcccacccgc
     2701 atgccccgcg cgcagagccc agggcgcacc gtcaactcga tcgccttgtc ggccgggcga
     2761 atgtccacaa ccacgccggt ttcctcgaca atgccagtaa acatagacag gcagaccatg
     2821 ttgagcgagc cgtccggatt caagcgaata aggcatccga ccagaggagg gctggacagc
     2881 gcatccgcag gttgtcggtg agaatggagc atgcgccgcg cggggcctgc ctgccgcgcc
     2941 tgcctgtgcc gtgcccgaca cggcagacag gccgctgcgc tctgcggcgc aggcagggca
     3001 cgcgggctgc agcgagagtc tccatccgtc gtaggcgcgg tgccctcacc ggccgacaca
     3061 agcatcgaca actgtcggat gcaccgacgg ccggagtctg tcgttgaatt tcgccgttcc
     3121 gcggctagtc tgatggcagt ttgtgaaaca acgcactgcg tcggggggct ccccgcaact
     3181 cgattcgtat gactgataca aatgccggtt ctgatcaacc gaagcagccg cgtcgccttg
     3241 tgcgatgggc cggcgggctc ggggtagctc tcgttttgct ggtggtggcc ctgtatttcc
     3301 tggcgatcag cgaaccgttt gtcaaaggcg tggttctgcc aaaggccggc gccgctctgg
     3361 gtgtggacat tgccgcggac ggcgtctcga tccgtccctt gtcggaggtg attctgcgcg
     3421 gcctcaaagt ccggccccaa ggcgaggaga cggttctcga ggccgccgaa ctgcgcgcgc
     3481 gctacaacct cgtctcgatt ctccgaggcc tgatcaaggt cgatgaaatc gcgctcatcc
     3541 aaccggtcgt gcgggtgaca atgcgtcccg atggaacgtc gaatctggac gcgttcaccg
     3601 gcaggagcgg cacgcccgag gccgcgtcgg ccaagcccgg ttctccgctg aaggtggatg
     3661 tcaggatgat ctcgatcgac gatgccaccg tccatttcac ccagacccac aaggacggca
     3721 gcgcaacgtc ggccgagctg gccggactcg atgccaaggc ctcggggatt cggaatggga
     3781 gcccggccaa actcgacctc gacggtcgca ttcagcttgt gcagcgcaca ggcacgaatc
     3841 aggccgatgt cgcaggcacg ctcaggagcg cgttcggaat cgatctcgac tccgacttgg
     3901 cacctgcgca gatcacaggg agcgcggatt tcgctgtcac ccgagccacc ggagcgttcg
     3961 ccgatgcgtc cgagttacag ggccgattgt cggcggactt caatctgagc gaaatcaagc
     4021 aactcgccat cgccttcacc aagggcgccg ccgccctggg gaccgcgagc gtgagcggcc
     4081 cgttcgatcc gctcgagcag gagggcaagc tcaaagtgaa ggtcaccggc attggcagcg
     4141 aactcctgag ccttgtcgga ggacgtttcg gcatctcctt cggcggaacg aagttggcgg
     4201 cggactacga catcgagttg aagcagaacg cccgattcgt gagcaccacc ggctcgatca
     4261 ccgccacgag cttcagcgtc gagcgggctg gtctgaggac gccgacagtc gacttcaagc
     4321 tcggttgcga cgcgaccctg gacctgcctc gaaccaacgc cacgatccga gcgttcaccc
     4381 ttgacgccac gcagtcgggc cgcccgttgc tgacgggcaa gctgacaaag gaaatgcttc
     4441 tcgattggag tcagggggcc caggaagccg cagagtccgc gttgaggttg aacgtcaccg
     4501 aactgaacct ggccgactgg aagccgttcg tgggcgactc cgtcgattcc ggcatagtca
     4561 acggccagct cgatatcaac gttcagaagg ccgggcgcct ggtggcgttt gacctgggtt
     4621 cgaagctcgt ggggctgacc ggccggctgg ggagcaatcg ccttgaccag gccgatctgg
     4681 ccgtcagcct gcgcggtcaa ctgaccgagt tcgaccgggt ggatttcgcg gcgttggctc
     4741 tggatatgtt ccaacggggc cgggctgtgt ccacgctcaa agggtctggc agttacgtga
     4801 actcgacctc cgacgccaac ttccaagtcg aactgacggc ttcgctggct gcgctgtccg
     4861 aattgctggg tcggacggac ctgtcgctca ccgcgggcac cgcaacgttc accggaaagg
     4921 tctcacagaa gaacctttcc cccgcccaga ccgcccatcc gagtctcgat cagagcctgg
     4981 ccgggaaact cgagatcgtt ggcctcatcg gaatccttgc atccaaccgc ctcgatcgtt
     5041 tcgatatttc gagcgatgtc gacgcgtcct tgaaaaacaa cctcgccgaa atccgcaagt
     5101 gcgtaggcag cattcgacaa gcggacaatc ccgcgggcac cttcaatctc acggggcgct
     5161 atggcctgac caacggtagc gggcagatcg cgctcgagtt cctgggtctg aatcagaacg
     5221 cgctgcagcc gtttgtgacc gaggcgctcg gcgacaagac gctcgcgtcg gtgacggtga
     5281 acggtcaaat cgccgggggt tacgatccgg ccggcgattc gcgcatccgg gctgacgtgc
     5341 agatcaccaa cctcgtggtc aacatcccga gcagcgcgcc ggcgtccacg ccgctcgaag
     5401 cgcagttcac actcgacacg gcggtgcgca ccaacgtcgc ggagatacgc gagatgctgc
     5461 tcaccctcac gccgacgtcc cgcgccgcca atcgcatcca ggccacgggg cgggtcgatt
     5521 tcacccagac gaacgcggcc accggccacc tcgcaatcac ggccgacgcg ctcgacgcca
     5581 cccgctgcta cgagattctc ttcgaggaga cggcgccttc cgcatcgggc gagtccgccg
     5641 gcggacggcc cggctcttcg ggcgctgcgg accaggagct ggaagcgatg accctcccgc
     5701 tgcgcaattt catcgtggac gccaacgttc ggcgtttcta tctgcgcgaa gtcgagatca
     5761 cgaacctcgt ggcgcgcgcg aggctggacg ggcagacgat cgaggtgaaa ccgttcgatt
     5821 tggcactcaa cggcgggtcg gtcagtgcga cggcaatcgt ggatttgggg gtgccgggat
     5881 acagatacga cgtcgggttc aagggaacca acgtccccgt ggcgccgctg gcgcggcggt
     5941 accggcggga ggggacgggc ccgggcagcg gcaccggcgc gggcttcgca cagtgccagg
     6001 gcgctggaac cacg
//
LOCUS       DAOQRO010000219         5993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13377_length_5993_cov_5.167060, whole genome shotgun sequence.
ACCESSION   DAOQRO010000219 DAOQRO010000000
VERSION     DAOQRO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13377_length_5993_cov_5.167060"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKK20_08880"
     CDS             <1..189
                     /locus_tag="PKK20_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00038.1"
                     /translation="RGHGEGHDRAICVPPRICVEFPTDSVHGTGRASQRIPTPIQGGT
                     QIPEEGKFFLKNCNPSGM"
     gene            289..1320
                     /locus_tag="PKK20_08885"
     CDS             289..1320
                     /locus_tag="PKK20_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNV00039.1"
                     /translation="MAQPVFHPSVEGAQHGAKTLAQFLDYAKASGAAGAQPSNYMLED
                     GKKFKSAKEIKDTFASRGMSLDGISCHCPIWVHTTAWTGSPTVRPFIPADVAKLAPDE
                     IEKWAEDYLLRFFDLAAELEVKILPMFWGVAFGWELATGYPWGFWKGGDYDLLEAGKE
                     RFVKKTAKIRKKANDLGLYLAHEIHPGTAAMCADDFNLLVSICDGDKCLTVNADPSHC
                     WEGESWETRFRKVASRVYACHVKNYTIRPNVPLRFMAPNWPDRAMQFVDISSGDLNMV
                     RYVEMLINIGYAARYCEIMGKKTAPLIVEAESAYRDIDATSANGIAYVRDNLCFPIAA
                     GSFEDGMGA"
     gene            1677..3374
                     /locus_tag="PKK20_08890"
     CDS             1677..3374
                     /locus_tag="PKK20_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV00040.1"
                     /translation="MSDRVVTTSSNNSLPKAAPPKLGRVFWTLNTIEMWERLAFYNLR
                     VMAPIYIMQADNPGGLHLTAGDKGIIYAWWAVFQSLLPVVTGGLADRFGYKRTLTFAI
                     SLMLIGYLMMAGLRDLASLDQFAALLSGIGRGIADWSFGASLPGRMLDGLTDFVGGGL
                     IGSGNYWGFFLSILTLATGTAFFKPGIQASLAHQLTRDNSSIGWGIFYWVVNVGAFVG
                     HFLPSLFLLKGLFWAGANSPEAWRNLFLASAFFTSFNLFLLLTFKDIPSGASKTESVG
                     QVLKRTLVNITEPRLLAWMAIMSCFWLMMYQLWDLQPNFIADWVDSGPAAAALGWLPE
                     GLRKVVVEQTPRGPMIPQQVILSCNALFIILGVVGIAWLTQRMRTLSAMLLGMIMATL
                     GVLIAGWTQSAWILVAGILCFSIGEMMTGPKKSEYLGLIAPPGKKGLYLGYVNIPVGV
                     GVFAGSWIAGWVYGRWGEKAVLALRYLAENTPYGAGKNWDGRIDMLEQTLGVPRTQAM
                     AKLQEATGLDAVGATQLLWTTYAPHIIWIPFACVGIAAAVALFIFGRMARRWSDMNA"
     gene            complement(3658..4074)
                     /locus_tag="PKK20_08895"
     CDS             complement(3658..4074)
                     /locus_tag="PKK20_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HNV00041.1"
                     /translation="MKPCLLKALTLIALTAALSGCKTETGALPPLNTTKFDTENRSNF
                     VLMDKGAQRSVTCSGIQQTTLPDGRLEVAAQVRNREKRRLDVQVNCEFKDEQGYVVDS
                     TPWQTLILTENGQETVRFTSMNNHAKRYTVRVRQSR"
     gene            complement(4071..5261)
                     /locus_tag="PKK20_08900"
     CDS             complement(4071..5261)
                     /locus_tag="PKK20_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00042.1"
                     /translation="MGRAVRCRLLWIWPAVVMAAGCSSPQPAFRPTGDVSVDGPKALA
                     IGPAQDRVLWQYRMASAAMRRAQFAEARQQLDGALARIGSIFDADAASRRARGYFTAE
                     AKKTFLGEPYERVMAYYYRGILYWMDGEPDNARACFRSAQFQDADAEERTYASDYVLL
                     EYLDGLATMKLKGDGSDAFARARSFCRLAIPPAANPAHNVLVFIDYGQGPIKIAAGQY
                     GEQLKFQPGHSQAREVMIRIGAQSAHVGPYDDLNFQATTRGGRVMDHILGNKAVFKAG
                     TDAVGNAAILSGAMIAGTGGGRGSANEVGAGLMIAGVLSKVVSAATTPRADTRTWESL
                     PLYLSFASFHIAPGRHTATVDFLDAERRPIAGLTKTITFTVASGSRDTVLYVSDKSTH
                     PQTL"
     gene            complement(5481..>5993)
                     /locus_tag="PKK20_08905"
     CDS             complement(5481..>5993)
                     /locus_tag="PKK20_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV00043.1"
                     /translation="ADERGFVVGTGIESQDIVTVADKMARGMMALPQINQAPVPPYVV
                     LDPVINETRFPINKDIFLTRMMTQLTSRSGGKVIFLARERMEALQKERDMKQQGQVTA
                     SSDPNIQEFRGADYFLTGKLQGLSTRTSKGTSDYVLYSFQLIDARTTAIVWAGDHEIK
                     RQGLEDAVYR"
BASE COUNT         1087 a         1796 c         1929 g         1181 t
ORIGIN      
        1 cgaggacacg gagaaggcca tgaccgggca atctgtgttc ctccccgaat ctgtgtggag
       61 ttccccaccg attcagtgca cggcacgggt cgagcgagcc agagaattcc cacaccgatt
      121 cagggcggaa cacagattcc cgaagagggc aagttcttcc tcaagaactg caacccttca
      181 ggaatgtaaa tgaaataacc gtggtgtgaa gcggggttga gcggaaaatg tgttgcgcgg
      241 catgtcaaaa atcggtatgt gtgcaggcca catttaacca atatccttat ggctcaacct
      301 gtttttcatc ccagcgtgga aggcgcccag cacggcgcga agaccttggc ccagttcctc
      361 gattatgcaa aggcatccgg cgcggcgggc gcgcagccgt ccaattacat gctcgaggat
      421 ggcaagaagt tcaagtcggc caaggagatc aaggacacct tcgcgtcgcg cgggatgtcg
      481 ttggacggaa tctcgtgtca ttgtcccatc tgggtgcaca cgacggcctg gaccggcagc
      541 ccgaccgtgc ggccgttcat acctgccgat gttgccaagc tggcgccgga cgagatcgag
      601 aagtgggcgg aggattacct gttgcgtttc ttcgatttgg cggcggaact ggaggtcaag
      661 attctgccga tgttctgggg tgtggccttt gggtgggagc tggcaaccgg ctatccgtgg
      721 ggtttctgga aaggcggcga ctacgacctg ctcgaagcgg ggaaggagcg attcgtcaag
      781 aagacggcga aaatccgcaa gaaggccaac gacctcgggc tttatctggc ccatgaaatc
      841 cacccgggca cggccgcgat gtgcgccgac gacttcaacc tgctggtgtc gatttgtgac
      901 ggcgacaagt gtctcacggt caatgcggat ccgtcccatt gctgggaggg cgagagctgg
      961 gaaacccgct tccgcaaggt ggccagccgc gtttacgcct gccacgtcaa gaactacacg
     1021 attcgcccga atgttccgct gcgcttcatg gccccgaact ggcccgaccg cgccatgcag
     1081 tttgtcgaca tctcgtccgg cgatctgaat atggtccgct acgtcgagat gctcatcaac
     1141 attggctacg cggcgcgcta ctgcgagatc atgggcaaga agaccgcgcc gttgattgtc
     1201 gaggcggaga gcgcctatcg cgacatcgac gcgaccagcg ccaatggcat cgcctacgtc
     1261 cgggacaacc tctgcttccc gattgccgcc ggatcgttcg aggacggcat gggggcttga
     1321 tcgcaagacg ttccaacagg cgagtcacag tcagcgcccc cgctttcgac atgaatgcgg
     1381 gggcttgctg tatttgggca tggtccgcgc gccccagact gctcctaacc cgcatatttc
     1441 acactgcgct ggtgcacagg gcactcggac ttcgcaagaa gactcccatg cgggtttgac
     1501 aagcaaaacc gggccaggtt cgagagttag agaactgcaa cacactgttc ccatggcaga
     1561 ccactctcag gagcgacaga atgcttccgg ttccctttcc gggcgcggtt ttgccacagc
     1621 ccggatatga aactccgcga ggctttgtgc aatgtacggg ctaaaccaag gtccacatgt
     1681 ctgacagagt tgtgacaaca tcctcgaaca acagcctgcc caaggccgcc ccgccgaagc
     1741 ttgggcgggt cttctggacc ctgaacacca tcgagatgtg ggagcggctg gcattctaca
     1801 acctgcgtgt gatggcgccg atctacatca tgcaggcgga taatcctggc ggcctgcacc
     1861 ttacggccgg ggacaaggga atcatttacg cctggtgggc cgtgtttcaa tcgttgctgc
     1921 cagtcgtcac cggcggcttg gctgaccggt tcgggtacaa acgcacgctg accttcgcaa
     1981 tcagcttgat gctgatcgga tacctgatga tggccggcct gcgcgacctc gcctcgctgg
     2041 atcaattcgc tgcgctgctc tccggcatcg gccggggcat cgcggactgg agttttggcg
     2101 cctctctgcc aggtcggatg ctcgatggct tgaccgattt cgtgggcggc ggcttgatcg
     2161 gctcggggaa ctactggggg ttcttcctga gcatcctgac cctggcgacg ggaacggcgt
     2221 tcttcaagcc ggggattcag gcctcactgg cgcatcagtt gacccgggac aactcatcga
     2281 tcggctgggg catcttctat tgggtggtga acgtcggggc gtttgtgggc catttcctgc
     2341 cctcgctttt cctgctcaaa ggcctgttct gggcgggcgc gaacagcccg gaagcctggc
     2401 gtaacctgtt cctggcttcg gcgttcttca cctcgttcaa tctcttcttg ctcctgacct
     2461 tcaaagatat cccgtccggc gcgtccaaga cggagagtgt cggacaggtg ctcaagcgga
     2521 cactggtcaa tatcaccgag ccgcgtttgc tggcgtggat ggcgatcatg tcgtgctttt
     2581 ggctgatgat gtatcagctc tgggatcttc agcccaactt catcgccgac tgggtggaca
     2641 gcggcccggc ggccgcggcg ctgggctggc tgccggaggg cttacgcaag gtggtggtgg
     2701 agcagacgcc gcggggtccg atgatcccgc agcaggtgat tctgagctgc aacgccttgt
     2761 tcatcatcct gggcgtcgtc gggatcgcct ggctgacgca gcggatgcgg acgttgtccg
     2821 cgatgttgct gggcatgatc atggccacgc tgggcgtgtt gatcgcgggc tggacgcaga
     2881 gcgcgtggat tctcgtggcg ggcattctct gcttctcgat cggcgaaatg atgaccggac
     2941 ccaagaagag cgagtacctc ggcctgatcg cgccgcccgg aaagaagggg ctttacctcg
     3001 gctacgtcaa catcccggtg ggtgttggcg tgttcgccgg ctcatggatt gccgggtggg
     3061 tgtacggacg ctggggtgag aaggccgtgc tcgccctccg ctacctggcc gagaacacac
     3121 cgtacggcgc cgggaagaac tgggatgggc gcatcgatat gttggaacag acgctgggcg
     3181 tcccgcgcac ccaagccatg gccaagcttc aggaagccac aggcctcgat gcggtcggcg
     3241 ccacccagtt gctctggacc acctacgccc cgcacatcat ctggatcccc ttcgcatgcg
     3301 tcggcatcgc cgcggccgtg gcgctcttca tcttcgggcg catggcccgg cgctggagtg
     3361 acatgaacgc ttagagtctg tgcaaagatt acttccgatt ttggcgggag cgccaccggg
     3421 ccagaagcaa ggcccgaggc agggagtgta tcccctgaga tactcgaccg acggaggtac
     3481 gacgcagatg gcccggtgcc gccccgcccc gaagggctgg gaggctttgg ctagctggcc
     3541 ccacggacgc cagattgctc ctcggtcaca gaccgttgcg ggtatgctcc ctcgtcgcgc
     3601 ctggccagcg agccaaatcc cctcgagcaa aaccggaatt agtttttccg cagaccctta
     3661 gcgcgactgg cgcacgcgga cagtgtagcg cttggcatga ttgttcatcg aggtgaaacg
     3721 caccgtctcc tgaccgttct cggtcaggat cagtgtctgc cacggggtcg agtcgacgac
     3781 atatccctgc tcgtctttga actcgcagtt cacctggaca tcgaggcggc gtttctcccg
     3841 attccggacc tgggcggcca cttcaagccg tccgtcgggg agcgtggttt gctgaatccc
     3901 cgagcatgta accgaccgct gcgcgccctt gtccatgagg acgaaattgg atcggttctc
     3961 ggtgtcgaat ttcgtcgtgt tgagcggcgg gagcgcgccg gtctcggtct tgcagccgct
     4021 cagcgcggca gtcagggcga tcagtgtgag ggctttgagc aagcagggtt tcatagagtc
     4081 tgtgggtgtg tggatttgtc gctcacgtac aggacagtgt cccgcgaacc ggacgcaacg
     4141 gtgaacgtga tggttttagt caggcccgcg atcgggcggc gctcggcatc caggaaatca
     4201 accgtcgccg tgtgccggcc cggggcgatg tgaaacgagg cgaagctcag gtagagaggc
     4261 aggctctccc atgtccgagt gtccgcccgg ggtgtcgttg cggcggacac caccttgctg
     4321 agcacaccgg cgatcatcaa gccggcgccc acctcgttgg ccgagccgcg tccgcccccg
     4381 gtgccggcga tcatggcccc gctgaggatg gcggcgttgc cgaccgcgtc ggtgccggcc
     4441 ttgaacacag ccttgttgcc caggatatgg tccatgaccc gcccgccccg ggtggtagcc
     4501 tgaaaattga ggtcgtcgta ggggcccaca tgcgccgact gcgcgccgat gcggatcatc
     4561 acttcccggg cctgagaatg gccgggctgg aacttgagct gctcgccgta ttgacccgcg
     4621 gcgatcttga tcggcccctg gccgtagtcg ataaacacca gcacgttgtg cgccggattc
     4681 gcagcgggcg ggatggcgag ccggcagaac gaacgggcgc gcgcgaaagc gtcggaaccg
     4741 tcgcccttga gcttcatcgt ggccaagcca tcgaggtatt cgagtagcac gtaatcgctc
     4801 gcgtaagtcc gctcctccgc gtccgcatcc tgaaactgcg ccgatcgaaa gcaggcccgg
     4861 gcattgtcgg gttcgccgtc catccaatag agaatgccgc ggtaatagta ggccatcacc
     4921 cgttcgtatg gttcgcccag gaatgtcttc ttggcctcgg cggtgaaata cccccgggcg
     4981 cgacgcgacg cggcgtcggc gtcgaagatc gatccgatgc gagccagggc cccatcgagc
     5041 tgctgtcggg cctcggcgaa ttgggcgcgg cgcatggccg cggaggccat gcggtactgc
     5101 cagagaacgc gatcctgggc cggcccgatc gcgagcgcct tcgggccgtc caccgaaacg
     5161 tccccggttg gccggaacgc cggttggggc gacgagcacc cggcggccat gaccacggct
     5221 ggccagatcc agaggaggcg acaccggacc gcgcgtccca tggggtcggg acacggccgg
     5281 cgacttgccg tttcgagggc gcgatccggg ctagaactag aatccttgct cggtgagaca
     5341 tgttgcagaa aagtcaagtt tctgtgctgc gttaaacggg tcaacggttc caatgagcga
     5401 ggtgcgggac agaggggcgg ggagacacgc ggactcctca tgggaaggcg aggactggat
     5461 ttggtctgag gcgcgcctcg ctagcgatac accgcgtctt cgagtccttg acgcttgatc
     5521 tcatggtcgc cagcccaaac gatcgcggtg gtgcgcgcat cgatgagctg gaagctgtag
     5581 aggacatagt cactcgtgcc cttcgatgtc cgcgtcgaca ggccctggag cttaccggtc
     5641 aggaaatagt cggcgccccg gaactcctgg atgttcggat ccgacgaggc tgtgacctgt
     5701 ccctgctgct tcatatcccg ctccttctgg agcgcttcca ttcgctcccg tgccaggaaa
     5761 atgaccttcc cgcccgagcg cgacgtcaac tgggtcatca tacgggtcag gaagatgtcc
     5821 ttgttgatgg gaaagcgcgt ctcgttgatc accgggtcca acacgacgta aggcggcact
     5881 ggcgcctggt tgatttgggg cagtgccatc atgccccgcg ccattttgtc ggccaccgtg
     5941 acaatgtcct gggactcgat cccggtgccc acgacgaatc cgcgctcgtc ggc
//
LOCUS       DAOQRO010000220         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13379_length_5992_cov_4.001853, whole genome shotgun sequence.
ACCESSION   DAOQRO010000220 DAOQRO010000000
VERSION     DAOQRO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13379_length_5992_cov_4.001853"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PKK20_08910"
     CDS             complement(<1..168)
                     /locus_tag="PKK20_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylglucosamine 2-epimerase"
                     /protein_id="HNV00044.1"
                     /translation="MTSDLTDPSSRARLAALYRATLLENVIPFWLDHGLDSEHGGILT
                     ALDRDGTVIDTD"
     gene            complement(165..803)
                     /locus_tag="PKK20_08915"
     CDS             complement(165..803)
                     /locus_tag="PKK20_08915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNV00045.1"
                     /translation="MDIRCLAANEHALAVPFPGHPDVSACQESSVVKLPDGRLFCVMR
                     TASGSPFWSVSADLGETWTQPRRLLRKDGAEPLLHPLSPCPIYDMGGNTACSGRYTLF
                     IHNHDGHFQGYGPTDTGFHRRPVCLAPSRFQPGAEQPVRFDAPRLFMDHSGVSLDKPG
                     TPGRLDLALYSSFTVRQGKAVLWYPDRKFFLLGRAIGEEWFDLGPTDSSKMP"
     gene            complement(816..2075)
                     /locus_tag="PKK20_08920"
     CDS             complement(816..2075)
                     /locus_tag="PKK20_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV00046.1"
                     /translation="MEASSQSSASRTAWLIVALLVPVALLNYLDRQMLAAMKFSVMED
                     IPDIALEENWGKILALFKWVYAFLSPIGGYLADRFSRRHVIAGSLFVWSAVTWTTGHV
                     TTYDQLLVTRAVMGVSEAFYIPAALALIADFHLGPTRSRAVGLHQMGIYAGVIIGGFS
                     GYAADSPALGWRWAFDTCGIVGVLYAMPLFFLLRNPPRPADSIERPHTSPASALRELL
                     GNSSFILLVLYFTLPALAGWVVRDWMPAILKAEFNIGQGKAGVSATLYWQVAAIIGAV
                     TGGWLADRWMRRTRRGRIYVSAIGMSLIVPAMFGVGYAPRTGLLGVALAFLILFGLGW
                     GFFDCNNMPILCQIARPELRATGYGIMNLVSISCGGLADWGFGILRDRQVPLFGIFGI
                     FASAAVISVVLVLLIRPRELDAPQQTS"
     gene            complement(2120..2272)
                     /locus_tag="PKK20_08925"
     CDS             complement(2120..2272)
                     /locus_tag="PKK20_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00047.1"
                     /translation="MDVSYACASRKTEQPAVRHARQTPSMQRFLELGDSAIPLNREST
                     PDGARL"
     gene            2283..3719
                     /locus_tag="PKK20_08930"
     CDS             2283..3719
                     /locus_tag="PKK20_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNV00048.1"
                     /translation="MSNHDNHPTSQLPDYITSAKPLGAEARAPWYKNVAPTYAGIFLW
                     FVFWQDAVKSDGLGGALSQGLGVALLGLVLAAAICHFLFYLVPGLLGMKTGLPLYIVG
                     TSTFGTQGGLIMPGFLMGILQFGWLGVNAFASSLALSAVIPIKAEILMVVWGFLAAFV
                     GLKGIQYVAKVSTYLPLIPLIILVLMLFKTLGTVGSFEPQTIIDAHRTVLPNAPAALS
                     AFGVIAFLLTYVVGFFATAGAAGVDIAMSSRNESDVKKGGLVGIALATLVTAGISALV
                     VAGVFGSKELSAQAVAAAQAAAENKDQFLLRSTNMISVVMGSGLGKWLLFALAVAAFP
                     SACFSALIAANSFKTTMPKVNPFLSVGIGATVSVILAVTGLAGKVVPVFVIIGASFGP
                     ICGAMMVDYLLAGQKWSGPRAGFNPAGWIAWALGFIVGILPNLGCNVPAAPVAAFIVG
                     AVVYWICAKSGMMSAVVPLPAANTPANK"
     gene            complement(3904..4779)
                     /locus_tag="PKK20_08935"
     CDS             complement(3904..4779)
                     /locus_tag="PKK20_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV00049.1"
                     /translation="MNIHHLELFYYVARHKGISEAVRNIPYGIQQPAVSAQVIQLEDD
                     LGLTLFHRRPFALTKAGEELYEFVKPFFDNLDSIGERIRGGMAQAIRIGSSEVVQRDH
                     LPEVVLNVRKQYPQLKLFLREGYQPQIESWLLRQDIDLAITIWEGRLPAGIHHEELIR
                     LPLLLMVSKNNPLKSAADLWKRDRIDEPLITLPACEAICRHFRRELEKRNIDWYPSLE
                     VGSLGLVETYAANGFGIGLGIHIPKAAHPPGLRLLPLTEFPPIVVGALWTGKRTALMD
                     CFIQEGHRRAKSLGG"
     gene            complement(4815..5345)
                     /locus_tag="PKK20_08940"
     CDS             complement(4815..5345)
                     /locus_tag="PKK20_08940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNV00050.1"
                     /translation="MIVKRNPLNWAERLYLPAVWNGFKVTLRHFFRRKVTMQYPEERW
                     VVPQAYRGAPYLVRDQNGNTKCVSCQLCEFVCPPKAISITPPGPDGPPADRRNAEKMP
                     LEFKINMLRCIFCGLCEEVCPEEAIFLKRDYALVGTNRQEMVYARDKLLELGGTHQDH
                     ILKWERMEREAAAQEP"
     gene            complement(5345..>5992)
                     /locus_tag="PKK20_08945"
     CDS             complement(5345..>5992)
                     /locus_tag="PKK20_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNV00051.1"
                     /translation="VYGIVLAGYAARSKYPLMGGIRSGAQMISYEIAMGLSLIPLFLL
                     VGDLNLGAVIDYQARGLFGWLIFKQPLAFAIFLVAAFAETNRLPFDMPEAEQELAGGY
                     NIEYSSIKFALFFMAEYAHMIAASAMLVTLFFGGWTLPFFGLDQPASTFWGGLLHIGI
                     FLAKLLAMMVLFVWVRWMWPRFRYDQLMALGWTRLVPLALGNIVVTALWLWLKAG"
BASE COUNT         1122 a         1785 c         1985 g         1100 t
ORIGIN      
        1 gtcggtatcg atgaccgtgc cgtcgcggtc gagcgcggta aggatgccgc cgtgctcgga
       61 atcgagtccg tgatcgagcc agaacggaat gacattctcg agcagggtcg cgcggtagag
      121 ggcggcaagc cgagcgcgac tcgaagggtc ggtgagatcg gatgtcatgg catcttcgaa
      181 gaatcggtcg gtcccaggtc gaaccactcc tcgccaatcg ctcgtcccag caggaagaac
      241 ttgcgatccg ggtaccagag gaccgccttg ccttggcgca cggtgaaact cgagtagagg
      301 gccaaatcga gccggccggg cgtgccgggt ttgtccaggc tcactccgct gtggtccatg
      361 aacagtctgg gtgcgtcgaa ccggaccggc tgctccgccc cgggctgaaa gcggctgggg
      421 gcaaggcaga ccggacgcct gtgaaagccg gtgtccgtgg ggccgtagcc ttggaaatgg
      481 ccgtcgtggt tatggatgaa gagggtgtag cgcccgctgc aggcggtgtt gccacccatg
      541 tcgtagatcg ggcaggggga gagcggatgc aggagcggct cggcgccgtc cttgcgcaga
      601 agacgccgcg gttgggtcca cgtctcgcca agatcggcgc tgacactcca gaacgggctg
      661 ccggacgccg tccgcatcac acagaagagc cgtccgtcgg ggagcttcac gacacttgac
      721 tcctgacagg cgctgacgtc cggatgcccg ggaaaaggca cggccagcgc gtgctcgtta
      781 gcggcgaggc aacggatgtc caaatcctgc acggcttaac ttgtttgttg aggcgcatcc
      841 agttcacggg ggcggatgag gaggacaagc acaacggaga tcacggcggc gctggcaaag
      901 atgccgaaga tgccaaagag cggaacctgg cgatcgcgca ggatgccgaa tccccaatcg
      961 gccaagccgc cgcaactgat gctgacgagg ttcatgatgc cgtagccggt ggcgcgcaac
     1021 tcgggccgcg cgatctggca gaggatgggc atgttgttgc aatcgaagaa accccagccg
     1081 agcccgaaca gaatgaggaa ggcgagggcg accccgagca atccggtccg cggcgcgtag
     1141 cccacgccga acatggccgg cacgataagc gacatgccga tggcgctgac gtagatgcgt
     1201 ccccggcgag tgcggcgcat ccaacggtcg gccagccagc cgccggtcac ggcgccgatg
     1261 atggcggcca cctgccagta gagcgtggcg gagacgccgg ccttgccctg gccgatgttg
     1321 aactcggcct tgaggatggc aggcatccag tcgcgcacga cccacccggc gagggccggc
     1381 aaagtgaagt agaggaccag gaggataaac gaactgttgc ccagcagttc acggagcgcg
     1441 ctggccggcg aggtgtgtgg gcgttcgatg ctgtcggcgg gacggggagg attgcgcagg
     1501 agaaagaaca ggggcatggc gtagaggacg ccgacgatgc cacaggtgtc gaacgcccag
     1561 cgccagccca gcgcggggct gtcggcggcg taaccgctga agcctccgat gatcacgccg
     1621 gcgtagatgc ccatttgatg gagtccgacc gctcgggaac gggtcgggcc gaggtggaaa
     1681 tcggcgatta gggccagtgc ggcggggatg tagaaggcct cgctcacgcc catgacggcc
     1741 cgcgtgacga ggagttggtc gtaggtggtg acgtggccgg tggtccaggt gaccgccgac
     1801 cagacgaaga ggcttccggc aatgacatgg cggcggctga aacgatcggc gaggtagccg
     1861 ccgatgggac tgaggaaggc gtagacccac ttgaagaggg cgagaatctt cccccaattc
     1921 tcctcgagcg cgatgtccgg gatgtcctcc atgactgaga acttcatggc ggccagcatc
     1981 tggcggtcga gatagttcaa gagcgccacc ggcaccagga gcgccactat gagccacgcc
     2041 gtgcgcgagg cggaggattg cgaagaggct tccataatcg gttcggtcgt gacactcgag
     2101 atgttcgtgc gggcacagtc tacaggcgcg cgccgtcagg cgtcgactct ctgttgagcg
     2161 ggattgcgga gtcgcccaac tccaggaacc gctgcatcga gggtgtctgg cgggcgtggc
     2221 ggacggcggg ttgttccgtt tttcggcttg cgcacgcata agaaacgtcc ataaaaacgc
     2281 gcatgagcaa tcacgacaac catcccacca gtcagctacc ggactatata acgagcgcga
     2341 aaccgttggg ggctgaagcg cgggccccct ggtacaagaa cgtggctccg acctatgccg
     2401 gcatcttcct ttggttcgtt ttctggcaag atgcggtgaa gtcggacggc ctgggcgggg
     2461 cgctgtcgca gggattgggc gtcgccctgc tcggtttggt gctggccgcg gcgatctgcc
     2521 atttcctgtt ctacctggtg cccggcttgt tgggcatgaa gaccggtttg cccttgtaca
     2581 ttgtgggcac atcgaccttt ggaacccagg gcgggctgat catgccgggc tttctgatgg
     2641 gcatcttgca gttcgggtgg ttgggcgtga acgcattcgc ctcctcgcta gccctgagcg
     2701 cggtgattcc gatcaaggcg gagattctca tggtagtgtg gggtttcctg gcggcttttg
     2761 tcggtttgaa aggcatccag tatgtggcca aggtctcgac ctacctgccg ttgattccct
     2821 tgatcatcct cgtcctgatg ctgttcaaaa ccctcgggac tgtgggcagc ttcgagccgc
     2881 agacgatcat tgacgcgcat cggacggttc tcccgaatgc ccctgccgct ctgagcgcgt
     2941 tcggcgtgat cgccttcctg ctcacatacg tggtcgggtt cttcgccacc gccggcgcag
     3001 ccggggtgga catcgcaatg agcagccgga acgagagcga cgtcaagaag ggtggtctgg
     3061 tcgggatcgc gctggcgacg ctggtgacgg cgggcatctc cgcgcttgtg gtggcgggcg
     3121 ttttcggctc gaaggaactg tcggcgcagg cggttgcggc ggcccaggcg gctgccgaaa
     3181 acaaggatca gtttctgctg cggtcgacca acatgatcag cgtggtcatg ggcagcggcc
     3241 tcggcaagtg gctcctgttc gcgctggcag tggcggcgtt cccgtcggcc tgcttctcgg
     3301 cgctgatagc agccaacagc ttcaagacaa ccatgcccaa ggtgaatccc ttcctctcgg
     3361 tcggcatcgg cgcgaccgtc agcgtcattc tcgccgtgac aggcttggcg ggcaaggtcg
     3421 ttcctgtttt cgtgatcatc ggcgcctcgt ttggcccgat ctgcggcgcg atgatggtgg
     3481 attatctgct ggcaggtcag aagtggagcg gtccccgcgc gggcttcaat ccggccggct
     3541 ggatcgcatg ggcgctgggt ttcatcgtcg gcatcctgcc gaacctcggc tgcaacgtgc
     3601 cggctgcgcc cgtcgcggcg tttatcgtcg gcgccgtggt ttactggatt tgcgccaagt
     3661 caggcatgat gtcggccgtt gtcccgctgc ccgcggcgaa tacgcccgcg aacaagtaag
     3721 ttctcttctc ctttgcacga cggtggctcg ccgcgcaggc tgttcgggat cgcgcggcga
     3781 gccggcctgt tgtcccatcc gcgccatctc cttgccgcgg gtgagtcggt tcatatcaag
     3841 gcatagcagt cctcttgcgc agtctgatcg ccatgtcctt ggacgtggtg tgtaatatgc
     3901 gggttagccg cccagggact tcgcgcgccg gtggccttcc tggataaagc agtccatcag
     3961 cgcggtccgc ttccccgtcc atagggctcc gaccacgata ggtgggaact ccgtgagtgg
     4021 gagcaggcgc aggccgggcg ggtgagccgc tttggggata tggatgccca ggccgatgcc
     4081 gaacccgttg gctgcataag tttcaaccag gcccaacgaa ccgacttcga ggctgggata
     4141 ccaatcgatg ttccgcttct cgagttctcg ccggaaatgg cggcagatcg cctcgcaggc
     4201 cgggagggtg atgagcggct cgtcaatccg gtcgcgcttc cacaaatcgg cggcgctctt
     4261 gagtggattg ttcttcgaga ccatgagcag gagaggcagc ctgatgagtt cctcgtgatg
     4321 aatgccggcc ggcagccgcc cctcccagat tgtgatcgcc aggtcgatgt cttgccgaag
     4381 cagccaagac tcgatctgcg gctggtagcc ttccctcagg aacaacttca attgcgggta
     4441 ctgcttccgc acgttgagca cgacctcggg caggtgatcg cgctggacca cctccgacga
     4501 cccgattcga atcgcctgcg ccatgccacc ccgaatgcgc tcgccaatgc tgtcgaggtt
     4561 gtcaaaaaac ggtttgacaa actcgtacaa ctcctcgccc gccttggtca gggcaaaggg
     4621 acgccggtga aacagggtca gaccgaggtc gtcttcgagc tggatcacct gggcgctgac
     4681 ggccggttgc tggattccgt aagggatgtt ccgcacggcc tcgctgatgc ccttgtggcg
     4741 ggcgacgtag tagaagagct ccaagtggtg aatattcatc ggctccagga ataccgggcc
     4801 tgaggcgcgc ctcgttacgg ctcttgagcc gccgcctcgc gctccatgcg ctcccatttc
     4861 agaatgtggt cctggtgtgt cccgcccaac tcgagcagct tgtctcgggc gtagaccatc
     4921 tcctgccgat tggtccccac cagggcatag tcgcgcttga ggaagatcgc ctcctcggga
     4981 cagacttctt cgcacaggcc gcagaagatg cagcggagca tgttgatctt gaactcgagg
     5041 ggcatcttct cggcgttgcg gcggtcggcg ggggggccat ccgggccggg cggtgtgatc
     5101 gagatggctt tgggagggca aacgaactcg cagagctggc acgagacgca tttggtgttg
     5161 ccgttctggt cgcgaaccag ataaggcgcg ccgcggtaag cctgaggcac gacccatcgc
     5221 tcttccggat actgcatggt cactttgcgt cgaaagaaat gacgcagcgt gaccttgaaa
     5281 ccgttccaga ccgcaggcag atagagccgc tcggcccaat tcagtggatt tcgtttgaca
     5341 atcattaccc tgccttcagc cagagccaca aggccgtgac cacaatgttc cccagcgcca
     5401 gcggcacgag ccgcgtccag cccagcgcca tcagttgatc gtagcgaaac cgcggccaca
     5461 tccaacgcac ccacacgaac aagaccatca tcgccagaag tttggcgagg aaaatgccga
     5521 tgtgcagcag cccgccccaa aaggtcgaag ccggctgatc caacccgaaa aacggcagcg
     5581 tccacccccc aaaaaacagc gtcaccagca tcgccgacgc ggcgatcatg tgcgcgtact
     5641 cggccatgaa gaagagtgcg aatttgatcg agctgtactc gatgttgtag cccccggcca
     5701 attcctgttc cgcctcgggc atatcgaatg gcagccggtt ggtttcggcg aaagccgcca
     5761 ccaggaagat cgcgaacgcg agaggctgtt tgaagatcag ccagccgaac aggccgcgcg
     5821 cctgatagtc gatcaccgcg cccaggttca aatcgcccac aagcaggaac agcgggatca
     5881 gcgaaagccc catcgcaatc tcgtacgaga tcatctgggc gcccgagcgg atgcccccca
     5941 tcaacgggta tttcgagcgt gccgcgtagc cggccagcac gatcccgtac ac
//
LOCUS       DAOQRO010000221         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13420_length_5980_cov_4.505485, whole genome shotgun sequence.
ACCESSION   DAOQRO010000221 DAOQRO010000000
VERSION     DAOQRO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13420_length_5980_cov_4.505485"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..795
                     /locus_tag="PKK20_08950"
     CDS             37..795
                     /locus_tag="PKK20_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00052.1"
                     /translation="MQLNTSNGIALEDGTDDDHSYLYAFDSDGKLKWKVQGGGILSKT
                     VPVIPPAPYAGTGLFAYARADGHHRDSGERQFGLILSVDADGNELGRWESDRAIWDCV
                     AWDLVGDGRLEVLATDLSGQLLRFDRELKSCSRMQICPEGPNGAVVQFVGLTNLTGQA
                     SRQVVLASWQVEKGKPVMGNDPNELSQEFWHELTIWVFDSKLRLRAKHRVAESLRAGH
                     ERPFHLADMDRNGIPEIVYFGADNVQVFTYRVGL"
     gene            1247..2092
                     /locus_tag="PKK20_08955"
     CDS             1247..2092
                     /locus_tag="PKK20_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV00053.1"
                     /translation="MDIKRAVITAAGPEHRMLPLQTLMDRDGVQKTALGILIEEVLGA
                     GIQEIGVVVCPGDQGAYRAAGGAPGTRLHFLEQSEPRGYGHAVACARDFTGRDPFLLL
                     VCDHLYVSRSVKPCAQQLVEVARAEGCSVSAVQATHESKLPYYGAVGGRLADARPGLY
                     KIDTVIEKPTPTEAEQKLVVPGLRAGHYLCFFGLHVLTAGAMALLTDLVDAAAPDQRV
                     TLSAALSALAGCERYLACELEGRRFDVGAKYGLLMAQFALALEGEDRDEVLAGLVELL
                     ALRRP"
     gene            2097..5426
                     /locus_tag="PKK20_08960"
     CDS             2097..5426
                     /locus_tag="PKK20_08960"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNV00054.1"
                     /translation="MSSRLIPMILSDDPSVRDRPLDACCAGLSLSELLGECAALEAFR
                     KQSESLYARVRALFFLYAIHRFHLPRRQEIANRGLIPFDGYEHLLQRRFEEAIASFLH
                     AQASSGPSDALSSALAAAYHRLAFQTLADQVRRSVRSVRGNQWMFRMGHPEDQPLRIR
                     PELLRRRCASEPYPVLRERTPVRMDLSHCGWSDIFFLGMDYPEGARVLNVSIDLGVRG
                     RDAVTRPPVEAYVRVLDEPVLRLTSIDLGATVDITNLVEVFDFARDYLGLLKAAVIAA
                     GIVPPGLEGSGQSLEQLLHRIVAPARGLEIVSNVNGIPKGSRLAVSTTLLASLIAVCM
                     RATGQAASLTGSLEESERRLVAARAILGEWLGGSGGGWQDSGGVWPGIKLIEGVRAGE
                     GDLEHGISRGRLLPDHRILGVDDAPPVAQQRLQESLVLVHGGMAQNVGPILEMVTEKY
                     LLRSATEWAGRQAALGILDEILTALRQGDIAAIGAATTRNFQGPIQAIIPWATNYYTE
                     TLIARVRAEFGDAFWGFWMLGGMSGGGMGFIFSPAAHRRAQTRLQSIMAAAKQELEHA
                     LPFAMEPVVYDFAINERGTWAELWVGGDALLPAGYYGLTVPALLKNELDALVPVRRAE
                     LDALAEACRSNSAYSGLAHQLIERLIPRMRPAAAGEERLPDLLSRHGFDPVHHEQIRA
                     DLRSARIGLAQNRLPANTEVQDVRAEDVCDATAPLGSELRAAGEEMLARGAVAVVSLA
                     AGTASRWTQGAGVVKALHPFCQLGGAHRTFIETHLAKSRRTSRACGAWLPHVFTTSYL
                     THEPIEAFLARQRRYGYEGPLVLSPGRAVGLRLVPMVRDLQFAWEEMPQQRLDEQAQK
                     VRDSLRAALIRWAESAGEGSDYTDNAPAQCLHPVGHWYEVANLLRNGTLQRLLAARPN
                     LKHLLLHNIDTLGAGVDPALLGLHATRGGCLSFEVIARRLEDRGGGLARVNDRIRLVE
                     GLAMPREEDEFRLTYYNTLTTWIDIDRLLAVFKLSRGNLDQIGKITTAVRDIAARLPT
                     YVTLKEVKKRWGHGQEDVFPVAQFEKLWGDMTALPEVDCRFFVVPRQRGQQLKDPAQL
                     DGWLRDGSAAYVESLCDWGSA"
BASE COUNT         1026 a         1814 c         2032 g         1108 t
ORIGIN      
        1 caaaggggcc cagcttcgga atactatcgt taatctgtgc aactaaacac tagtaacggg
       61 attgcgcttg aagacggcac tgacgacgac cacagctacc tctacgcgtt tgattccgac
      121 ggcaagctca aatggaaggt gcagggaggc ggaatcctca gtaagaccgt gccggtcatc
      181 ccgccggcgc cgtacgcggg gacgggcctg ttcgcttatg cgcgtgcaga cgggcaccat
      241 cgcgacagcg gcgagcgcca gtttgggctc atattgagcg tggacgccga cggaaacgaa
      301 ctggggcgat gggagagtga tcgggctatt tgggattgtg tggcgtggga cttggttgga
      361 gacggacgcc tcgaggtcct cgccaccgac ctgagcggtc aacttctccg cttcgatcgt
      421 gagctgaaat cctgctcaag gatgcagatc tgcccagagg gtcccaacgg agccgtcgtg
      481 caatttgtgg ggctgacaaa cctgactggc caggccagtc gccaggtggt cctggccagt
      541 tggcaggttg agaagggcaa gcctgtgatg ggaaatgacc cgaacgagct ttctcaagag
      601 ttctggcacg agctgacaat ttgggtgttt gacagcaaat tgaggctccg tgccaaacac
      661 cgggtggccg agagcctgcg agcaggacat gagcgaccgt ttcacttggc cgatatggac
      721 cgtaacggca ttccggagat cgtctacttc ggtgcggaca acgtgcaggt gttcacgtat
      781 cgagtaggcc tctgaactgc gggtgccccc caggaaagca acatctggaa gaccagagaa
      841 cgggtgagga aggtcgggac acgaggtccc attctcgatt tgacctttgc acaacgacgg
      901 aaggcagggc ggtcagtccc ctgaccgcca ctatgtcgat gccccggtgc gccgttcatg
      961 gtgtggtcgc gctgtgttca ctcgaagttc ccctctcgta atcgtaatcg taatccactg
     1021 tcggcgacga ggattacgat tacgattagg accaccccca cggttcatgg gcggcgttga
     1081 cattggacgc gcgaatccgc actgggccca tgaaccagaa acgttcaact gtctcccatc
     1141 tcgaccctgc tctctgtcct ctgctctatt tggccacggt tgacgcacac actgcaagtg
     1201 tggaattctc ggtgcatcga agcgcaactc gaaaggggca aagactgtgg acatcaaacg
     1261 agcggtcatc acggctgccg gcccggagca tcggatgctg ccgctgcaaa cgctgatgga
     1321 tcgggacggc gtgcaaaaga cggcgctggg catcctgatc gaggaggtgc tcggcgcggg
     1381 catccaggag atcggggttg tcgtgtgtcc gggcgaccag ggggcgtatc gcgcggcggg
     1441 cggagcgccg ggcacccgcc tgcactttct cgaacagtct gagccgcgcg ggtacgggca
     1501 tgctgtcgcc tgcgcgcgcg acttcaccgg gcgcgatccg ttcctgcttc tcgtgtgcga
     1561 ccatctgtac gtgagccgct ccgtgaaacc gtgcgcgcaa caattggtcg aggtcgctcg
     1621 agccgaaggc tgctcggtct ctgccgtgca ggccacgcac gaaagcaagc tcccgtacta
     1681 tggggcggtc ggcgggcggt tggctgacgc gcgtccaggc ctgtacaaaa tcgacacggt
     1741 gatcgagaag ccgacgccga ccgaagcgga acagaagctt gtggtgccgg ggctgcgggc
     1801 gggtcattac ctgtgtttct ttggtctgca cgtcctgacg gccggggcga tggcgcttct
     1861 gaccgacctg gtcgacgcgg cggctccaga ccagagagtg acgctctcgg cggcgttgtc
     1921 ggcactggcc ggatgcgagc gctatttggc ctgtgaactc gaggggcggc ggttcgacgt
     1981 cggggcgaag tatggtttat tgatggccca gttcgcgttg gcgctcgaag gcgaggaccg
     2041 ggacgaggtc ctggccgggt tggtcgagct tcttgccttg cgccgtccgt aaagccatgt
     2101 ccagtcgcct catccccatg atcctgtcgg acgatccgtc cgtgcgggac cgtccgctgg
     2161 acgcgtgctg cgcgggtttg tcgttgagcg agttgcttgg ggaatgtgcc gccctcgagg
     2221 ccttccgcaa acagagcgag agcctctacg cccgggtgcg ggcgctgttc ttcctttatg
     2281 ccattcaccg attccatctg ccgcgaaggc aggaaatcgc caatcgcggt ctcattccat
     2341 tcgacggcta cgagcatctt ttgcagaggc gcttcgagga ggcgatcgct tccttcctcc
     2401 atgcccaggc ctcgagcggg ccgagcgacg ccctctcgag cgccctcgcc gcggcctatc
     2461 atcgcctcgc cttccagacc ctggccgacc aggtccgccg cagcgtgcgc tcggtgcggg
     2521 gcaaccagtg gatgttccgc atgggccatc ccgaggacca gccgctgcgc atccgtcccg
     2581 agttgctccg tcgccgctgc gcgtccgagc cgtatcccgt gttgcgcgag cgcacgccgg
     2641 tgcgcatgga cctgtcgcat tgcggttgga gcgacatttt cttcctgggg atggactatc
     2701 ccgagggtgc gcgggtcctg aatgtctcga tcgacctggg ggtgcgggga cgcgatgctg
     2761 tcacacggcc gccggtcgag gcgtatgtgc gggttctgga cgagccggtg ttgcggctga
     2821 cgagcatcga tctgggggcg acagtcgaca tcacgaattt ggtcgaggtg ttcgatttcg
     2881 cgcgggacta cctgggcctg ctcaaggcgg ctgtgattgc ggcggggatt gtgccgcccg
     2941 ggctggaagg gtcgggacag agcctcgaac agttgctgca tcggattgtc gcgcccgcgc
     3001 gcggactcga gatagtgagc aacgtcaacg ggattccgaa ggggtcgcgc ctggcggttt
     3061 cgacgaccct gctggcttcg ttgatcgccg tctgcatgcg ggcgacgggc caggcggctt
     3121 ccctgaccgg ctcgctcgaa gagtcggagc ggcggctggt tgccgcgcgt gcgatactgg
     3181 gtgaatggct gggcgggtct ggcggcgggt ggcaggattc gggcggtgtc tggccgggaa
     3241 tcaaactgat cgagggggtg cgggcgggcg agggcgacct cgagcacggg atcagccggg
     3301 ggcgtttgct gcccgaccac cgcattcttg gcgtcgacga tgcgccgccc gtggcgcaac
     3361 agcggctcca ggaaagcctg gtcctggtcc atggcggcat ggctcagaac gtcgggccca
     3421 ttctcgagat ggtgaccgag aaatatctgc tgcggtcggc cacggaatgg gctgggcgtc
     3481 aggcggcgct gggtattctg gacgagattc tgacggcgct gcgtcaaggc gatatcgccg
     3541 cgattggcgc cgccacaaca cgcaactttc aggggcccat tcaggcgatt atcccctggg
     3601 ccaccaacta ctatacggag actctcatcg cccgggtgcg ggccgagttt ggcgacgctt
     3661 tctgggggtt ctggatgctc ggaggaatgt cggggggcgg aatggggttc atcttctcac
     3721 ccgcggccca tcggcgtgcg cagacgcgcc tgcagtcgat catggcggcg gcgaaacagg
     3781 aactcgaaca cgcccttccg tttgcgatgg agccggtggt gtacgatttt gcgatcaacg
     3841 agcggggcac atgggccgaa ctctgggttg gtggcgatgc gctgctgccc gcgggctact
     3901 acggcctgac cgtgcccgcg ctgctcaaga acgaactcga cgcgctcgtc cccgtccgcc
     3961 gcgcggaact cgatgccttg gccgaggcat gtcgcagcaa ttccgcctat tccggtctgg
     4021 cgcatcaatt gatcgaacgg ctcatcccgc gcatgcgtcc ggcagcggcg ggcgaggagc
     4081 gcctgcccga cctgctttcg aggcacggtt tcgacccggt ccatcacgag cagattcggg
     4141 ccgatctgcg cagcgcgcgc attggcctgg cgcagaaccg gctgcccgcc aatacggagg
     4201 tccaggatgt ccgcgccgaa gacgtgtgcg acgcaaccgc cccccttgga tcggagcttc
     4261 gggctgccgg cgaggagatg ctggcgcggg gagcggtggc cgtcgtgagc ctggcggccg
     4321 gcaccgccag ccgctggacc cagggagcgg gcgtggtcaa ggccctgcat ccgttctgtc
     4381 agttgggcgg agcgcatcgg actttcatcg aaacgcacct ggccaagagc cgccgcacga
     4441 gtcgcgcgtg cggcgcctgg ctgccgcacg tgttcacgac gagttacctg acgcacgagc
     4501 ccatcgaggc attccttgcc cgccagcggc ggtacggcta cgaagggccc ctggttttgt
     4561 caccaggtcg ggctgtcggc ttgcggcttg tcccgatggt ccgggacctc caattcgcct
     4621 gggaagagat gccccaacag cgccttgacg aacaggccca gaaagtccgc gacagcctgc
     4681 gggcggccct gattcgatgg gccgagagcg ccggcgaagg gagcgattac accgacaatg
     4741 cgcctgcgca gtgcctgcat ccggtcgggc attggtacga agtcgccaac ctgctgcgca
     4801 acgggacgct gcagcgtctg ctcgccgcgc gtccgaacct caagcacctg ctgctccata
     4861 acatcgacac cctgggtgcc ggcgtcgatc cagccctgct gggtttgcat gcgacgcgcg
     4921 ggggctgcct ctccttcgag gtgatcgctc gccggttgga agaccggggc ggaggtttag
     4981 ctcgagtcaa cgatcgcatc cggttggtcg agggcttggc catgccgcgc gaggaggacg
     5041 agttccgcct cacctactac aacaccctca ccacctggat cgacatcgac cggctcctgg
     5101 cggttttcaa gctgtcgcgc ggcaacctgg atcagatcgg gaagatcaca accgccgtcc
     5161 gcgacatcgc cgcccgtctg ccgacctatg tcacgctcaa ggaagtcaag aaacgctggg
     5221 gccacgggca ggaggacgtc tttccagtcg cccagttcga gaagctttgg ggcgacatga
     5281 ccgctctgcc cgaggttgac tgccggttct tcgtcgtccc gcgccagcgc ggccagcaac
     5341 tcaaggatcc ggcgcaactg gacggctggc tgcgcgacgg ttccgccgca tacgtcgaga
     5401 gtctgtgcga ttggggctcc gcgtgaatcg gccttggctc gccttgcctt ctcagcctgc
     5461 gcgccaagag gcgaacgccg tgccactcgc aggatagatc gatgctgcca ggtgtatggg
     5521 atgggcgaca aataggagga actggtgggc aactacgcca atacaggcct gcaatggctg
     5581 ccggccaaac gaccgcgccg ggtccagggg cacgacttcc cggggccgga ggcgccggcg
     5641 ccaaacgcga aattttcgga cttccagatc atctcttgat tgcgtggcgc ggcggcgatc
     5701 cgctactgag gaaaacgggg tgatgaactt ggattcgata gacgaaccag cttgccgtcg
     5761 cgccgattgg tgacagcgag ttcgacgcaa tgcgccgccg gcagtcgtca gggcgagctg
     5821 tccgcttcga cggaagaaag cggcttgctc tccgtcaact ggcgcaggat gaaccggcgc
     5881 cggttgacga gttggtcgcg gaacgactcg atatcgccgc gaaaccgcct cagggcgacc
     5941 tcggtgtccc gacgttcgat cgaggctctg tagacgatct
//
LOCUS       DAOQRO010000222         5953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13495_length_5953_cov_4.742625, whole genome shotgun sequence.
ACCESSION   DAOQRO010000222 DAOQRO010000000
VERSION     DAOQRO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13495_length_5953_cov_4.742625"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..2326)
                     /gene="priA"
                     /locus_tag="PKK20_08965"
     CDS             complement(92..2326)
                     /gene="priA"
                     /locus_tag="PKK20_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNV00055.1"
                     /translation="MIMIARVTLEIALRKEFDYLIPPELAGQVEVGSWVKVPFGARQV
                     FGCVTALVETSPCAKLRPLLRVVGRRSLMTPRVLELARWIAGYYCCHVETAIKSVLPE
                     AVRREEEGWRERLFVRALPVSGPLPKLSKRQAEIHGIIGERKEMPLQELLRMTGTTSE
                     TVRRLEARGLVTVAAQVSERDPYAHEVILPTSPLVLNPEQRQAIDAITRALDTSRDGA
                     RHAPFLLHGVTGSGKTEVYLQAIARALALGLGAIVLVPEISLTPQTVERFKARFCSGR
                     LQTLVAVLHSHLSVGERHDEWHKIRQGRARIVIGARSAIFAPVEPLGLIIVDEEHEHS
                     YKQEEAPRYHARDVAVVRAQREGAVAVLGSATPSLESYYNARRGKYVLLELPSRVDNQ
                     KLPVVRVVDLCREYRGDKGIPILSNLLREAVTRRLERKEQVILFLNRRGYATSLQCPR
                     CGYVANCPNCSVALTYHRSRQQLLCHICGHQKPAPTACPEASCRNPAIRYAGLGTQRV
                     EETLRRVFPRAQIQRMDSDVLKRKEDYRRILGDFRTGKIDILLGTQMIAKGLHFPNVT
                     LVGIIHADLTLHVPDFRSAERTFQLLTQVSGRAGRGDVEGEVIVQSFTPYHPAIQYAK
                     RHDFVNFYEQEIEFRTQLKYPPISRIALLTLKGRNQDKVKFTAAHVKRELDSALAGFK
                     DAVLAGPAPAPLLRAETLYRHQIMLRTRQLAKASALLAEIIDRLSLPEEVTLTVDIDP
                     VNLT"
     gene            complement(2364..4334)
                     /locus_tag="PKK20_08970"
     CDS             complement(2364..4334)
                     /locus_tag="PKK20_08970"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNV00056.1"
                     /translation="MKLARLLEIGCGLALLMGLSGQGEPPALSDLYRLDRLPVLRDHA
                     KVGAVTSYDRSGGNDDGFSGKYSFVRKDAEGLVLADLQGPGVITRIWTPTPTDDTLEF
                     LFDGEPQARLQVGFRDLFLGHHPGFPQPLVGFGAGGYYCYVPIPFAKSCVVRLRAAKA
                     QFYQINYVQCAPDASVTSFGPLKAGTQQPDLEKAVELWRSAGQDIARFSAPPGARLET
                     ARRSFVLSPNASEAAYRAEAGGRIVGLRVGPASAIAGKARDLLLRIVFDGTEPCVEVP
                     LGDFFGYAWGQPATHGLLVGTMGETNYCYYPMPFERQATVEIVSQRSVPVALWTEVVH
                     TPIARTAIEGMFYALWRRENPTKIGEPYEFLDTKGRGHLVGVVLQSQGFESGKTLFFE
                     GDDQTTIDGTLAVHGTGSEDFFNGGWYDVPDRWEKRISFPLSGCLGYQKHLGRTGAYR
                     FFLADAYPYRQSLLQTIEHGGEDNSIITDYCSVAWFYSEGRPTTSLAAPPLGQRAVSD
                     PQEIVFPMWWQTPIYAWSFDRASLTRKKAQIGGEEVRYLSLAAEGQDWFGPHFISPTI
                     DVPAAGVYAVFIEAVKGPAQGMVQLFQNENPVGEPVDLHAAEPVKSERLLLGRLVLAE
                     GRNDLMLKLPGKNERSSGLGLDLVQIVCVREQ"
     gene            4651..5391
                     /locus_tag="PKK20_08975"
     CDS             4651..5391
                     /locus_tag="PKK20_08975"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNV00057.1"
                     /translation="MNVKPDRTAWLKGGTAQFPATPRPGARPCRLILLGAPGVGKGTQ
                     AELLSERLGPSHLSTGDVFRAAKSLNSAERSPALNAALDAMRRGELVSDETVIEMVRE
                     RASCLRDPGGFLLDGYPRTVAQAEALDQLLKREGLRLDAVLSYDLPIDTIVARLSGRR
                     TCGQCKAVFHVQTRPPKADGVCDHCGGALVQREDDRPEAVRVRMETYERSTAPLADYY
                     RQRGLLKSVVATGTPEEIYQRTVALLGA"
     gene            complement(5515..>5953)
                     /locus_tag="PKK20_08980"
     CDS             complement(5515..>5953)
                     /locus_tag="PKK20_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00058.1"
                     /translation="GRDCRIENNIFVDCAPAVHVDARAMGWAKYHADEWVKEGREKGT
                     LSGIRYTQAPYSDRYPKLPTILDEDPWAPRGNVIARNICVGGRWDDIESKARPLITFE
                     DNLLDADPLFVDAARGNFELKPESPAWKLGFRRIPIEKIGPDR"
BASE COUNT         1063 a         1934 c         1912 g         1044 t
ORIGIN      
        1 tggcgaggcg cgacgaggga gcatacccgc agcggtctgt gagcgaggag caatcactcg
       61 acctctgcca gggattccag cccggctttc gtcacgtgag gttcaccgga tcgatgtcca
      121 cggtaagcgt cacctcttcc ggcaggctta ggcggtcgat gatctcagcc aacagcgcgc
      181 tcgctttcgc gagttgtcgg gtgcgcagca tgatctggtg ccggtacagc gtctccgcgc
      241 gcaggagcgg cgccggcgcc ggcccggcca gcacggcgtc cttgaacccg gccaaagccg
      301 aatcgagttc ccgtttcaca tgggcggcgg tgaacttgac cttgtcttgg ttgcggccct
      361 tgagcgtgag caaggcgatg cggctgatgg gcgggtattt caactgcgtc cggaactcga
      421 tttcctgctc gtagaagttg acgaagtcgt gccgcttcgc gtactggatc gcgggatgat
      481 acggggtgaa ggactgcacg atcacctcgc cctcgacatc cccgcgcccg gcgcgtccgg
      541 acacttgcgt caacaactga aacgtccgtt ccgccgagcg gaaatccggc acgtgaagcg
      601 tcaggtcggc atggatgatc ccgaccaggg tgacgttagg gaaatgcagc cccttggcga
      661 tcatctgggt gcccaggaga atatcgatct tgcccgtccg gaaatccccg aggatgcggc
      721 ggtagtcttc cttccgcttg agaacatccg agtccatcct ttgaatctgc gcgcgcggaa
      781 acacgcgcct cagagtctcc tcgacgcgtt gggtgcccag gccggcatag cggatcgccg
      841 ggttgcggca cgacgcttcc ggacatgccg tcggcgccgg tttctggtgg ccgcagatgt
      901 ggcaaaggag ttgttgccgg gatcggtggt aagtcagggc cacgctgcag ttcgggcaat
      961 tcgcaacata gccgcagcgc gggcattgca gtgatgtggc gtagccgcga cggttaagga
     1021 acaggataac ctgttcctta cgttcgagcc ggcgtgtcac ggcctcccgc agcaggttcg
     1081 agaggatcgg aatccccttg tcgccgcgat attcccggca gaggtcgacc acgcggacca
     1141 cagggagctt ctggttgtcg acgcggctgg gcagttcgag gagcacatac ttgccgcggc
     1201 gtgcgttgta gtagctttcg agggacggcg tggccgaacc gagaaccgcc accgcccctt
     1261 cccgctgggc gcgaaccacg gccacatcgc gcgcatggta gcgcggggcc tcctcctgct
     1321 tgtacgaatg ctcgtgttcc tcgtcgacga tgatcagccc caacggctcg accggagcaa
     1381 agatggccga cctcgctcca atgacgatcc gggcccggcc ctggcggatt ttgtgccatt
     1441 cgtcgtggcg ttccccgacc gagaggtggc tgtgcaagac ggccacgagc gtttgcaggc
     1501 gtccggaaca gaaccgcgcc ttgaatcgtt cgacggtctg gggcgtgagc gagatttccg
     1561 gcacgagcac aatcgcccca agcccgagag caagggcgcg cgcgatggct tggaggtaga
     1621 cctccgtttt gccgctgccc gtcacgccgt gaagcagaaa gggcgcatgt cgggctccat
     1681 cgcgcgaagt gtcgagcgct cgcgtgatcg cgtcgatagc ctgccgctgc tcgggattca
     1741 agaccagagg cgatgtgggc agaatcacct cgtgggcgta gggatcgcgt tcggatacct
     1801 gcgcggccac ggtgaccagg ccccgggcct cgaggcgccg caccgtctcg gacgtcgtgc
     1861 cggtcatgcg cagcaattcc tgaagcggca tctccttgcg ctctccaatg atgccgtgaa
     1921 tctcagcctg gcgcttgctc aacttgggca ggggaccgct caccggcaat gcgcgcacga
     1981 acaagcgttc gcgccagccc tcttcctccc gccggaccgc ctcaggcaac acgctcttga
     2041 tggcggtctc gacatggcag cagtaatagc ccgcaatcca gcgcgccaac tcgagcacgc
     2101 gcggagtcat gaggctccgg cggcccacca cccgcaacaa tggacgcagc ttcgcgcacg
     2161 gcgaggtctc gaccagagcc gtgacgcatc cgaagacctg gcgcgctccg aacgggacct
     2221 tgacccaact gccgacctcg acttgccctg ctaactcggg aggaatcagg tagtcgaatt
     2281 ccttgcgcag cgcgatttcg agtgtgacgc gggcaatcat gatcatcccg accagcgggc
     2341 ctgtccacgc tcttcaaacc gattcactgc tcgcgcacgc agacgatctg gaccaggtcc
     2401 agtccaagcc cggatgagcg ctcgttcttg ccgggcagtt tgagcatcag gtcattccgc
     2461 ccttccgcca gcaccaggcg ccccaggaga aggcgttcgc tcttcaccgg ttcagcggcg
     2521 tgcaaatcca ccggttcgcc gaccgggttt tcgttctgga acaactggac catgccttgc
     2581 gctggtccct tgacggcctc gatgaagacc gcgtaaacgc ccgccgcggg aacatcgatc
     2641 gtcggcgaga tgaaatgcgg gccgaaccag tcctggcctt cggccgccag cgatagatac
     2701 cgaacctcct cgccgccgat ctgcgccttc ttgcgggtca ggctcgctcg gtcgaatgac
     2761 catgcataga tcggcgtctg ccaccacatc gggaagacaa tctcctgggg atcgctcacc
     2821 gcccgttgcc cgaggggcgg cgcggcgaga ctcgtggtcg gacgcccctc cgagtagaac
     2881 caggcgacac tgcagtaatc ggtgatgatg ctgttgtcct cacctccgtg ctcgatggtc
     2941 tgaagcaggc tttggcggta cggatacgcg tcggcgagga agaaccgata ggcgccggtg
     3001 cggcccaggt gcttctgata gccgaggcaa ccgctcaagg gaaaagagat gcgtttctcc
     3061 cagcgatccg gcacgtcgta ccacccccca ttgaagaaat cctccgaacc ggtcccgtga
     3121 acggccaggg tcccgtcgat cgtcgtctga tcgtctccct cgaagaacag ggtcttgccc
     3181 gactcgaatc cctgggattg cagcacgact cccacaaggt gaccgcgtcc cttggtgtca
     3241 agaaactcgt acggttcccc gattttcgtc ggattctcac gccgccacag ggcgtaaaac
     3301 atgccctcga tggcggtgcg cgcgattggg gtgtggacga cttcggtcca gagcgcgacc
     3361 ggcaccgagc gctgcgagac gatctcgacc gtcgcctgcc gctcgaacgg catcgggtaa
     3421 tagcagtaat tcgtctcccc catcgtcccc acaagcaggc cgtgcgtggc cggttgcccc
     3481 caggcgtatc cgaagaaatc cccaagcgga acctcaacgc atggctcggt cccgtcgaag
     3541 acaatcctca acagcagatc gcgagctttg cctgcaatcg ccgacgccgg accaacccgc
     3601 aatccgacaa tgcgcccgcc cgcctcggcc cgataggccg cttcagacgc gttgggactc
     3661 agcacaaaag agcggcgcgc cgtctcaagc cgcgccccgg gcggcgccga gaatcgggcg
     3721 atgtcctgtc cggcgctgcg ccacagttcg accgccttct cgaggtccgg ctgttgggtg
     3781 ccggccttca gcgggccgaa tgatgtcacg gaggcatcgg gcgcgcactg gacgtagttg
     3841 atctggtaga attgcgcctt ggctgccctc aggcggacca cgcacgactt ggcgaacggg
     3901 atggggacat agcagtagta gccgcccgcg ccaaaaccga ccagcggttg cggaaaaccg
     3961 gggtgatggc cgaggaacag atcgcgaaac ccgacctgca ggcgcgcttg cggctcgcca
     4021 tcgaagagaa actcgagcgt gtcgtccgtc ggcgtcgggg tccagattcg ggtgatcacc
     4081 cccggccctt gaaggtccgc cagcaccagt ccctccgcat ctttgcgcac gaacgagtac
     4141 ttcccggaga atccgtcgtc gttgcccccg ctccgatcgt aacttgtcac cgcgccgacc
     4201 ttggcgtggt cacgcaagac cggcaaacga tccagccggt aaaggtccga cagagccggt
     4261 ggctctcctt ggcccgacag ccccatgaga agtgccaggc cacagccgat ttcgagaagg
     4321 cgcgcgagtt tcatcggacg agacttaaca tgacgcgctc cggtccgcca gcccgcaccg
     4381 aggggcgcag cgccgtgcgg cgcatccgac agttgtcggg attctgtcgt ccggtgaggg
     4441 cagccgcccc gatcatgatc aatgcctgct ttggtgtagg ccgcggagtg catacgccgt
     4501 caatggacgg agcggtcgga ctccgcacga tgacggctat gcgggccagg ccaaaaaagg
     4561 tgaagcgatg ggcaatcccg gtgttgccgc ccgctgctct ggcgaatcga atgcgtccgg
     4621 gtgcggacag tcgcgctcat ggagaaacat atgaatgtca aacccgatcg aactgcatgg
     4681 ctcaagggcg gaacagcgca attcccggcg actccgcgtc caggggcgcg accctgccgc
     4741 ctgattctgc tgggggcgcc gggggtcgga aagggcaccc aggccgagtt gctgtccgaa
     4801 cgtctcggac cgagtcatct gtcgaccggc gatgtgtttc gcgcggcgaa gtcgctgaac
     4861 tcagccgaac gcagtccggc gctcaacgcg gcgctcgatg cgatgcggcg gggggaactg
     4921 gtatccgacg agacggtgat cgagatggtg cgcgagcggg cgagttgcct gcgtgacccg
     4981 ggcggttttc ttctcgatgg gtatcctcgg accgtggcgc aggccgaggc gctcgatcag
     5041 cttctgaagc gggagggcct caggctggat gcggtgctca gctacgatct gccaatcgat
     5101 accatcgtcg cgcgcttgag cgggcgccgg acctgtggcc agtgcaaggc ggtgttccac
     5161 gtccagactc ggccccccaa ggcggacggc gtgtgcgacc attgcggcgg cgccctcgtt
     5221 caacgcgaag acgaccgccc cgaggcggtg cgcgtccgca tggagaccta cgagcgaagc
     5281 accgctcccc tcgcggatta ttatcgccaa cgcggcctgc tcaaatccgt cgttgccacc
     5341 ggcacgcccg aggaaatcta ccagcgtacc gtcgccctcc tcggcgcgtg agggaacgcg
     5401 cgcccggcat ggcgcccagg tcgcggagcg ccccggagcg tcggcttgca gacggcttgg
     5461 cgagcgccct gcctgtggcg ttgaccaacc gcggcccggt gaggacggcg cgggttagcg
     5521 atcgggtccg atcttctcga tcgggatgcg tcggaatccg agtttccagg ctggcgactc
     5581 cggcttcaat tcgaagttgc cgcgggccgc atcgacgaac agcggatcgg catcgagcag
     5641 attgtcctcg aacgtgatca gcggccgggc cttggactcg atgtcgtccc aacgcccccc
     5701 gacacagatg ttgcgcgcga tcacgttgcc gcgcggagcc cacggatctt catcgaggat
     5761 cgtcggcagt ttcggatacc ggtcgctgta aggcgcctgg gtgtagcgaa tgcccgacag
     5821 agttcctttc tcccgcccct ccttcaccca ctcgtcggcg tggtacttgg cccagcccat
     5881 ggcccgggcg tcgacgtgga ccgctggcgc gcagtccaca aagatgttgt tctcgatgcg
     5941 gcaatcgcgt ccg
//
LOCUS       DAOQRO010000223         5951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13502_length_5951_cov_4.379919, whole genome shotgun sequence.
ACCESSION   DAOQRO010000223 DAOQRO010000000
VERSION     DAOQRO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13502_length_5951_cov_4.379919"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..2312)
                     /gene="rpiB"
                     /locus_tag="PKK20_08985"
     CDS             complement(141..2312)
                     /gene="rpiB"
                     /locus_tag="PKK20_08985"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNV00059.1"
                     /translation="MKKTVLFVCTGNVCRSPMAEGLFRHQTRHGGRYEVLSAGVGAID
                     GQPPSALAIEVMRELGIDISGQRSRALTSELAQRADYIFVMTHGHLDTVSLLHPHAVE
                     KTFLLREFDETLAVYEKDIPDPIGEGLSVYRDCRDHIEQGLHSLLAFIEQTAGGGDSP
                     PISHRPMTITVGADHAGYALKELLKQHLALHGYEVNDVGAHSAESTDYPDYAQAVARQ
                     VVSGQSTFGLLVCASGVGMSIAANKVPGIRAAVARDDVMAALVRQHNDANVLCLGARF
                     VNEEQARRILTAFLGAKFEGGRHQRRVSKLEAGESRAARLARQDAAVHAAIRREVLRQ
                     QENIELIASENFASPAILEAQGSVMTNKYAEGYPRKRWYGGCENVDVVEQLAIDRARE
                     LFGAEHANVQPHSGSGANMAVYFAFLKPGDKMLTMDLSHGGHLTHGNRANFSGRFFEI
                     VHYGVRKDDERIDYDQLAGLAREHHPKMITVGASAYPRTIDFARMGEIAREVGALLLA
                     DIAHIAGLVATGLHPSPVPHADFVTTTTHKTMRGPRGGLILCRGQYAKEIDSQVFPGI
                     QGGPLMHVIAAKAVCLHEASRPEFKAYQERVVRNAAALAAGLRRNGFRLVSGGTDNHL
                     ALIDVGARGMTGKECQMALDEAGITVNKNTIPFETRSPFQASGIRVGSPAVTTRGMAE
                     AEMAAIADMISEVLMDIKNLDVAHQVRNRVHELTAKFPLPY"
     gene            2435..3349
                     /locus_tag="PKK20_08990"
     CDS             2435..3349
                     /locus_tag="PKK20_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV00060.1"
                     /translation="MRRTVFYVVVSVIVLVIVLSGTTASALWWYFHPSHERTHGIVYG
                     QRHGEPLVMDVARPARPNGLGVALMVSGGWKSGTNAFRPFMAAPLLRRGYTVFAVTHL
                     SQPDATVMEIFDDVSRAVRFIRHHAEEYGIDPDRIGVTGGSAGGHLSLMLATRGGPGP
                     ADAPDPVDRASSAVQAAAIFFPVTDLLNLGQSTENAGDGGPPKSFVKAFGPQSTNLAA
                     WKVIGRAMSPIYHVTSNMPPVLIVQGGADTLTPPEQSEWFRDRARETGARVELIVRPG
                     KKHGWLSMLWDIRLFADWFDRHLARASE"
     gene            3455..3631
                     /locus_tag="PKK20_08995"
     CDS             3455..3631
                     /locus_tag="PKK20_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00061.1"
                     /translation="MANRIWEGERPGTFRLPENPWLVQLFVLALRSYNFPFLGGQGGV
                     LILLPASLCRWPTR"
     gene            3680..4561
                     /locus_tag="PKK20_09000"
     CDS             3680..4561
                     /locus_tag="PKK20_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV00062.1"
                     /translation="MRGRVGAAPDRLQHTNCMGSMFENWGRAMGRLSMPAYQKAKWAW
                     QSLAGSEEDRLKAERELGGTLALKIRASSTAPAAAADFELVRRIGDALAARLKNRQIR
                     FRIDAIQLPEPSALALPGGYIFISVSLLDLCQRYEDEIAFVLGHEMAHVIRGHATDHY
                     LQESFMKALASRLGRATPLGGLVKDTTLQLLSSAYSQECEFEADEFGSRLAQAAGHHP
                     LASIRLLERLQHLHGDQTPLGQYFSSHPPPAERTARLRRVWSSRLREASHEAQTDCHR
                     PNAATGAPTARGISSRR"
     gene            complement(4501..5910)
                     /locus_tag="PKK20_09005"
     CDS             complement(4501..5910)
                     /locus_tag="PKK20_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00063.1"
                     /translation="MAKVTDIRVRSAELFFLPVHTRVPLKFGPETLTSVTCARARVRV
                     ENRHGRGATGWGETPLSVQWVWPSALPYDARHEALKRFCVRLAEAWPCFDADGHPIEI
                     GHAFQEHRLGALLEACNAERGPTVEPMPWLAALVCCSVFDQAIHDAYGHMLERPAYTT
                     YGADCLNRDLAHFLDPAAGATSDFRGRYPDAFLASEPQRTLKAWHLVGGLDPLDPSEL
                     TGLEPQDGYPVLLEDWIRTDGLTCLKVKLRGNDVPWDYQRLVRVGEIGRAQGVEWLSA
                     DFNCTVTDPAYVNGILDRLRAEHPRLDRMLLYVEQPFPYDLDRHRIDVRGVSARKALF
                     LDESAHDWKLIRLGRELGWSGVALKTCKTQTGAVLSACWAKAHGMALMVQDLSNPMLA
                     QIPHLLLAAHVGTIMGVETNGMQFYPAASNPEARVHPGVYRRRHGRVDLSTVRGPGFG
                     YRLEEIPRAVGAPVAAFGR"
BASE COUNT         1067 a         1977 c         1851 g         1056 t
ORIGIN      
        1 cattgatcgt gaacccggac cttcaccgtc aacggggaga acgtgtcgcc gttccggaaa
       61 gggcggtccc agcgtttcga gctgagggta aatacggttt gaccagcctt agccgactga
      121 gttccagtga gttccagact ctagtatggc aaggggaatt tcgcggtcaa ttcgtggaca
      181 cgattacgca cttggtgggc gacgtcaagg tttttgatgt ccatcagcac ctcgctgatc
      241 atatcggcga tcgcggccat ttcggcttcg gccattccgc gcgtcgtgac cgccggcgag
      301 cccacccgga tgccgctggc ctggaatgga gaccgagttt cgaaggggat ggtatttttg
      361 ttgacggtga tgccggcttc atcgagagcc atctggcact ccttgccggt catcccgcgc
      421 gcgcccacgt cgatcaacgc caggtggttg tcggtgccgc cgctcaccag ccgaaatccg
      481 ttgcgccgca ggccggccgc cagcgcggcc gcgttgcgga ccacgcgctc ctggtacgcc
      541 ttgaactcgg gccgcgacgc ctcgtgcagg cagactgcct tggcggcaat cacgtgcatc
      601 aaggggccgc cctgaatgcc cggaaacacc tgcgaatcga tctccttggc gtactggcca
      661 cggcacagga tcagcccgcc gcgcggcccc cgcatcgtct tgtgcgtggt ggtcgtcacg
      721 aaatccgcat gcggcaccgg gctcgggtgc agtccggtcg caaccaggcc cgcaatatgc
      781 gcaatgtccg cgagcagcag cgcgcccacc tcgcgcgcaa tctcgcccat cctggcaaaa
      841 tcgatcgtcc ggggataagc gctggcgccg accgtgatca ttttcggatg gtgctctcgc
      901 gccagcccgg ccagttgatc gtaatcgatc cgctcgtcgt ccttgcgcac cccgtagtga
      961 acgatctcga aaaagcgacc cgagaaattg gctcgattgc cgtgggtgag atgccctccg
     1021 tgactcaaat ccatcgtcag catcttgtcg ccgggcttga ggaaggcgaa gtagaccgcc
     1081 atattggccc cgctgcccga gtgaggctgc acgttcgcgt gctcggcgcc gaagagttcc
     1141 cgggcgcgat cgatggccaa ctgctcgacc acatccacat tctcgcatcc gccgtaccag
     1201 cgcttcctgg ggtaaccctc ggcgtacttg ttggtcataa ccgacccctg ggcctcgagg
     1261 atagccgggc tggcgaagtt ctcgctggcg atcaactcga tgttctcctg ctgacgcagc
     1321 acttcacgcc gaatcgccgc gtgaaccgcc gcatcctgcc gggccaggcg cgcggcgcgc
     1381 gactcacccg cctcgagctt gcttacccgg cgttgatgcc gacccccctc gaatttggcg
     1441 cccaaaaacg ccgtcagaat ccgccgcgcc tgctcctcgt tgacaaagcg ggcgcccagg
     1501 cagagaacgt tggcgtcgtt gtgctgccgc accagggccg ccatcacatc gtcccgcgcc
     1561 accgcggcgc ggattcccgg caccttgttg gccgcaatgc tcatccccac acccgacgcg
     1621 cagaccaaca atccgaaggt cgattgcccg gaaaccacct gcctggctac cgcctgcgcg
     1681 tagtccggat agtcggtcga ttccgccgaa tgtgcgccga cgtcgttgac ctcgtacccg
     1741 tgaagggcca ggtgctgttt aagaagttct ttcagtgcat accccgcgtg gtcggcgccg
     1801 acagtgatgg tcataggacg gtgtgaaatg ggaggggaat cgccgcctcc cgcggtttgt
     1861 tcgatgaaag ccagcaggga atgcagccct tgctcgatat gatcccgaca gtcccggtag
     1921 acggaaagac cctcgccaat cggatccgga atgtccttct cgtacaccgc aagcgtctcg
     1981 tcgaattccc gcaacagaaa cgtcttctcc accgcatgcg ggtggagcag gctcacggta
     2041 tccagatgcc cgtgagtcat cacgaagatg taatcggccc gctgcgccaa ctcggacgtc
     2101 agcgcccggc tgcgctgccc tgaaatgtca atcccaagct cgcgcatgac ctcgatcgca
     2161 agcgcgctcg gaggctggcc gtcgatggcc cccacccccg ccgacaggac ctcgtatcgg
     2221 ccaccatgac gcgtctgatg gcggaagagg ccttccgcca tcgggctgcg gcacacgttg
     2281 cctgtgcaaa caaagaggac tgtcttcttc atcctgacgc aaacgggcag aaattggagt
     2341 gcgcgccgcg aatcgtcaac ggagaaaccg aacgctcgcc ctcaattccc agcttggagg
     2401 cggacggatt tcatgccacg ttccggacat gtccatgcgc cgcacagtct tttatgtggt
     2461 cgtgagcgtc atcgtgctgg tcatcgtcct gtccggcacg acggcgtccg cactctggtg
     2521 gtacttccac ccctcgcacg agcgcaccca tggcattgtt tatggacaac ggcacgggga
     2581 acccctggtc atggacgtgg cgcggccggc gcggcccaac ggattgggtg tcgctttgat
     2641 ggtgagcgga gggtggaaat cgggcacgaa cgcgtttcga cctttcatgg cggcgccgct
     2701 cctgcggcgc ggctacacgg tctttgccgt cacccacctg tctcagccgg atgccacggt
     2761 catggagatc ttcgacgacg tgagccgggc ggtccggttc atccgacatc atgcggagga
     2821 gtatgggatc gacccagacc gcatcggagt gaccggcggc agtgcgggcg gccatttgag
     2881 tctgatgctg gccacgcgcg gcggccccgg acccgccgat gcgcccgatc ccgtggatcg
     2941 cgccagcagc gccgtccagg cggccgcgat cttcttcccg gtcaccgatc tgctcaacct
     3001 gggccaatcc acggagaacg cgggtgacgg cggaccgccc aagagctttg tcaaggcgtt
     3061 cgggccgcag tcgaccaatc tggctgcctg gaaggtgatc gggcgcgcga tgtcccccat
     3121 ctaccacgtc acgagcaaca tgcccccggt cctcatcgtg cagggaggcg ccgataccct
     3181 cactccgccc gaacaatccg agtggtttcg cgaccgcgcc cgagaaaccg gggcgcgtgt
     3241 cgaactcatc gtgcgccccg gcaagaagca tggctggttg agcatgcttt gggacatccg
     3301 gctgttcgcc gactggttcg accggcatct ggcccgcgcg agcgaatagg cacccggcgg
     3361 gcgaggaacg gcgccttgcg cggacgcggc gcggatcgcg ttttcagcta accggattcg
     3421 cattggttga tcggcaccct tctggtaaat ggcaatggcg aaccgaatct gggaaggcga
     3481 acgcccggga acgttccgcc tgcccgaaaa cccgtggctg gtgcagttgt tcgtcctggc
     3541 gctgcgcagc tacaacttcc ccttcctggg cggccagggc ggggtcctga tcttactgcc
     3601 cgcatccctc tgccgctggc caacacgctg acgagtccgc ttgccattcc gggattcacg
     3661 ctcaacagta tcaaagacga tgcgcgggcg cgttggtgcg gccccggatc gcctgcaaca
     3721 cacaaactgc atggggagca tgttcgagaa ttggggccgt gcgatgggcc gtttgagcat
     3781 gccggcctac caaaaagcca agtgggcctg gcagagcctg gccggctctg aagaggatcg
     3841 cctgaaggcc gagcgggaac tgggcggcac tctggcgttg aagatccgcg cgtcgtccac
     3901 tgcgcccgcc gccgccgcgg atttcgaact cgtgcgtcga attggagacg cgctggccgc
     3961 gcggctgaag aaccggcaaa tccgttttcg aatcgatgca atccaactgc cggagccctc
     4021 ggccctcgcg ttgcccggcg gatacatctt cataagcgtg tccctcttgg atctctgtca
     4081 acggtacgaa gacgagatcg cgttcgtcct cggtcacgaa atggcccacg tcatccgcgg
     4141 gcatgccacc gaccattacc tccaggaatc gttcatgaag gcgctggcga gccgactcgg
     4201 gcgcgcgact ccgctgggcg gcctcgtcaa ggacaccacg cttcagttgt tatccagcgc
     4261 ctactcgcag gaatgcgaat tcgaggcgga cgaattcggc tcgcgcctgg cccaggccgc
     4321 gggccaccat cccctggcga gcattcgcct gctcgagcgt ctccagcact tgcacggcga
     4381 tcaaacaccg ctcggtcagt acttcagttc ccatccgccc cccgccgagc gcacggcccg
     4441 ccttcggcga gtctggagca gcaggcttcg ggaagcttca cacgaagcac agacagactg
     4501 tcaccgcccg aatgccgcga ccggcgcccc gactgcgcgc gggatttcct cgaggcgata
     4561 gccgaacccg gggccgcgca ccgtgctcag gtcgacacgc ccgtggcggc gccggtaaac
     4621 ccccgggtgc acgcgcgctt ccggattgga cgccgccgga tagaactgca tgccgttggt
     4681 ctcgaccccc atgatggtgc ccacgtgcgc ggccagcagc agatgcggga tctgggccag
     4741 catcggattg ctcagatcct gcaccatcag agccatgccg tgtgccttgg cccagcacgc
     4801 gctgagcacc gcgccggtct gagtcttgca ggtcttcagc gcgacccccg accagcccag
     4861 ttcgcggcca agtcggatca gcttccaatc gtgcgcgctc tcatcgagaa acagcgcctt
     4921 gcgcgccgat acgccgcgga catcgatccg atgccggtcg aggtcatagg gaaaaggctg
     4981 ctcgacatag aggagcatgc ggtccagacg cggatgctcg gcacggagac gatcgaggat
     5041 accgttgacg taggccgggt cggtgaccgt gcagttgaaa tcggcgctga gccattcgac
     5101 gccctgagcc cgcccgatct cgcctacccg caccagccgc tgatagtccc aaggcacgtc
     5161 gttgccgcgc agtttcacct tcagacacgt caacccgtcc gtgcgaatcc agtcctccaa
     5221 cagcaccgga tacccgtcct gaggctcgag tccagtcagt tcggaaggat cgagcggatc
     5281 aagtccgccc accagatgcc aggccttcag cgtgcgctgg ggctcggacg caagaaacgc
     5341 gtccggatag cggccccgga aatcgctggt ggcgccggcg gccgggtcga gaaaatgcgc
     5401 gagatcgcgg ttgagacaat ccgcgccgta ggtcgtgtag gcggggcgtt cgagcatgtg
     5461 gccataggcg tcgtggatcg cctgatcgaa cacggaacag cagaccagag ccgccagcca
     5521 gggcatcggt tcgaccgtcg gcccgcgttc ggcgttgcag gcttcgagca atgcgcccaa
     5581 tcggtgttcc tgaaaggcgt gaccgatctc gatcgggtgt ccgtccgcat cgaaacacgg
     5641 ccaggcctcg gccaatcgca cgcaaaagcg cttgagggcc tcatggcgcg cgtcgtaggg
     5701 cagcgcgctg ggccagaccc attgcacgct cagcggcgtc tcgccccacc ccgtcgcgcc
     5761 gcgcccatgt cggttttcca cccgaacgcg tgcgcgcgcg caggtgaccg acgtgagcgt
     5821 ttccgggccg aacttgagcg gcacccgggt gtgaacggga aggaagaaga gttcggcgga
     5881 tcggacgcgg atatcagtga ctttggccac ggggtcaaag taggtagatt ctgcccgtct
     5941 gttcaagcct t
//
LOCUS       DAOQRO010000224         5941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13537_length_5941_cov_4.052837, whole genome shotgun sequence.
ACCESSION   DAOQRO010000224 DAOQRO010000000
VERSION     DAOQRO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13537_length_5941_cov_4.052837"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKK20_09010"
     CDS             <1..895
                     /locus_tag="PKK20_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00064.1"
                     /translation="ISFWKPKPDVGQYTTLALATCEAIRAADPHATIIGPATSEVPVK
                     FLEEFFASGVLAHLDAVSVHPYRNYARPPETAAEDYAKLRDLIRRYAPDGRKELPIIS
                     GEWGYASHTKGVSPETQAAYIVRQQLANLLAGIPVSIWYDWKNDGPDPEEREHNFGTV
                     WPDLKPKPAYAAIRTMTTALNGYRVARRLETGNSQDYVLVCETDGGPAKLVAWTIGEP
                     HEVRVPARAQGRVIVRATLGSGETTLGLETKRDDGIKEELYARCESDKDAMLRLTVDN
                     LGIGLGPMPVYVSLEGIALDR"
     gene            complement(1152..1934)
                     /locus_tag="PKK20_09015"
     CDS             complement(1152..1934)
                     /locus_tag="PKK20_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV00065.1"
                     /translation="MKTTSVLVGVLGLTFGVNAQTVFYDNFDSYADQAAFNAVWNVGV
                     AGGGLILSTDQASSGSQSVQQDLTAKASGIDITPVSGWDGAPLEWSFRFYDSTQSANL
                     RQFATIRDNAPALAQLVAIGAYNDTTLTKNLITSEPVTAADLNTYYAARVAFSPGPNW
                     FILNMDGVPTRSTGWHELKAVFSDTTVDFYVDGVLGMAGIEYAAAAGAVTFDRLTVGS
                     GLSSANGAAFYDDVKVTVIPEPTTLALLGLGALAFLGRARRE"
     gene            complement(2194..3789)
                     /locus_tag="PKK20_09020"
     CDS             complement(2194..3789)
                     /locus_tag="PKK20_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNV00066.1"
                     /translation="MDCHGDPDLTAVNEAGQTVSLHVDGAKYGASVHGAMPCAACHGD
                     ISRQHPDDNLAAKRVDCAACHEDQSSSHGARLHGAAAKDGARAARCSDCHGSHQVLPD
                     TSPDSTLHHSKISATCGACHPRAAQAVAESVHGLAAIRGERDAALCTDCHFEHAGAGA
                     DGASARRLSAQICGKCHAAEPITASFRPAPDPVRTFYQSYHGLAVQYGSTRAANCASC
                     HGYHRILPSHDPDSSIHPNRLVETCGKCHPGATENFARGRIHGDGGLGRDTGSLVNRW
                     VRSVYITIIFTVVGLMAAHNLTVWLLKAAAARRAPGRSVMRMDRTQRIQHMLLFVSFI
                     ALAITGFALKLPDSWMAWVLGADDAIRRGLHRIFGLLLLATAVWHVLYVLTTIEGKRL
                     FRDLWPRRGDVRDARDRIRALAGKTPVRPRTRRFDYVEKIEYWAVVWGVVIMGVTGLM
                     IWLKIDVTRILPRWAVEVATTVHYYEAILACLAVVVWHAYHVVFDPGVYPMNWAWWDG
                     RVADESCREESSLERRASDQSQS"
     gene            4110..4952
                     /locus_tag="PKK20_09025"
     CDS             4110..4952
                     /locus_tag="PKK20_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNV00067.1"
                     /translation="MPFAICNEIFGDWPIETAFRFAAQTGYDAIEIAPFTLANHVTDV
                     PAARRGEIRDAAARAGIAIAGIHWVLLKTEGMHLTHSERPVRERTARYFIDLVDFCAD
                     LGGHIIVVGSPKQRSLLPGVSFEQARESAVEVFRDAVRRGEEREVTICFEPLAPAETD
                     FINTAAEAVSLVQRFESPRFKIILDVKAMCSEARPIPDIIREAWPHFAHFHANDRNLK
                     GPGFGDVDFRPIAAALREVGYAGYVSVEVFDFTEGPEAIATRSLAYLRQAFGNPYSEA
                     RLAQ"
     gene            complement(5020..5739)
                     /locus_tag="PKK20_09030"
     CDS             complement(5020..5739)
                     /locus_tag="PKK20_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00068.1"
                     /translation="MRFVFRWLFRLLVVGLVLSIGVVLLKDLLLKELLASHIERETGL
                     ETRIGRLEVGILSPRLTVENLRLYNPPEFGGSVFLHVAECHVEYDRLALLQRRVHLRL
                     FRLNLAEASIVEDVAGQTNLESIHRRLRRKQASEPRSDFTFGGLDMLNLSLGRLHRLN
                     LAAPGRIQTINLGVQNEIYTNLDTEKDVLYALGRLALRIGVSQLTNHFPIAPDVSRPR
                     STEKAPAPLRPPATTPAPGRR"
BASE COUNT         1157 a         1796 c         1909 g         1079 t
ORIGIN      
        1 catctcgttc tggaaaccga aacctgacgt gggtcagtac acaacgcttg cgctggccac
       61 ctgcgaggcc atccgcgcgg ccgatccgca cgcgacaatc atcggcccgg caacgtccga
      121 agtgcccgtc aagttcctcg aagagttctt cgcctccggc gttcttgcac accttgacgc
      181 cgtcagcgtg catccctatc gcaactatgc cagaccgccc gagaccgcgg ctgaagatta
      241 cgccaagctg cgcgacctga ttcggcggta tgcgccggac gggcgaaagg aactcccgat
      301 catcagcggg gagtggggtt atgcgagcca caccaaaggc gtctcgcccg agacgcaagc
      361 cgcctacatc gtccgccaac aactggcgaa cctcctggcc ggcatcccgg tctcgatctg
      421 gtacgactgg aagaacgacg gccccgatcc cgaggagcgg gagcacaact tcggcaccgt
      481 ctggcccgat ctcaaaccga aaccggctta cgcggcgatt cggacgatga cgaccgccct
      541 gaatgggtac cgcgtcgcga ggcggctgga gaccggaaac agccaggact acgtcctcgt
      601 gtgcgagacg gacggcggcc cggcgaaact cgtcgcgtgg accatcggtg agccgcatga
      661 ggtcagggtc cccgcgcgcg cgcaagggcg ggtgatagtt cgcgcgactc tcggctcagg
      721 tgagaccacg ctcgggctcg agacgaagcg cgacgacgga atcaaggagg agttgtacgc
      781 ccggtgcgag tcggacaagg atgccatgtt gcgtttgacc gtcgacaacc tcgggatcgg
      841 tctggggccc atgccggtct atgtcagcct cgagggcatc gcacttgatc ggtagagcgg
      901 aatggagcgc gagggcgcgt acttgagacg atcggcgtca accgtcaggg ccgcttcagc
      961 gggcgcggga tcggccctcc gggagagcgt gaaggcgtga agggcgtgaa ggcgtgataa
     1021 tcaccctccc cccccgcggc ctctgaaacg gccttccggg agggcatatg caggagggag
     1081 gaatcccgga taaaagaaga agccgctggc gggttcgcag cggcttcgct gaatcagttg
     1141 tggttgccga gttactcgcg ccgggcgcgg cccagaaacg ccagggcgcc cagtccaagc
     1201 agagcgaggg tcgtcggctc gggaatcacc gtcaccttga cgtcgtcgta aaaggcggcg
     1261 ccgttggcgg agctgaggcc cgatccaacc gtgagtcggt caaaggtgac tgctccagcc
     1321 gcggcggcgt actcgatgcc cgccatgccc aggactccgt caacgtagaa atccacggtt
     1381 gtgtcggaga acacggcctt gagttcatgc caaccggtgc tgcgcgtggg cacgccatcc
     1441 atattcagga tgaaccaatt ggggccggga gagaacgcca cgcgggcggc gtagtaggtg
     1501 ttcagatcgg cagccgtcac cggctcgctc gtgatgaggt tcttggtcag cgttgtatcg
     1561 ttataggcgc cgattgcgac gagctgcgca agggcaggcg cgttgtcgcg gatcgtggcg
     1621 aactgacgca ggttggccga ttgcgtcgaa tcatagaagc ggaacgacca ctcgagcggc
     1681 gctccatccc agcccgagac cggtgtgatg tcgatgccgc tggccttggc ggtgaggtcc
     1741 tgttggactg actgagagcc gctggaggcc tggtctgttg ataggatcaa tcctccgccg
     1801 gcaacgccca cattccacac cgcattgaag gcagcctggt cggcgtaact gtcgaaatta
     1861 tcgtagaaga ctgtctgagc gttgactcca aaggtcagcc caagaactcc aaccaatact
     1921 gacgttgttt tcatagtgct cttcgcccgc tggtaaacct cagcaacacg ttgtctgcgc
     1981 aggacttccc ctgccttcat atggggggga tgattcgctt ttgcacgcct gtgtcaagcg
     2041 gaaatcgaca gcggaaattg acgtatcgac agcaaaaact gacgggcact cgggagcgag
     2101 accctcgggg aacgcggtcg ggccgcattc ggtggtctca atcgagcaag acggaagagg
     2161 agattcggga agacgtcgag ttgactatga ggttcaggat tgggattggt ccgaggcgcg
     2221 cctctcgaga gaactctctt cccgacagga ttcatccgcg actcggccat cccaccacgc
     2281 ccaattcatc ggatagacgc cggggtcaaa aaccacgtgg taggcgtgcc agacgacgac
     2341 ggccaggcac gcgaggatgg cttcgtagta atgaacggtg gtcgcaacct cgaccgccca
     2401 tctgggcagg atgcgggtca cgtcgatttt gagccagatc atcaggccgg ttacccccat
     2461 gatcacaacc ccccacacca cagcccaata ctcgatcttc tcgacatagt caaagcgtct
     2521 ggtgcgcggc ctgacaggcg tcttgccagc cagggcgcgg atccggtccc ttgcgtcgcg
     2581 cacgtcgccg cgccgcggcc agagatcgcg gaacagacgt tttccctcga tggtggtcag
     2641 gacgtacaga acgtgccaga ccgcagtggc caggagaaga agaccgaaga tgcgatgaag
     2701 cccccggcga atggcgtcgt cggcccccag gacccaggcc atccaggaat cggggagctt
     2761 gagtgcgaag cccgttatgg ccagggcaat gaagctgaca aacaggagca tgtgctggat
     2821 gcgctgcgtc cggtccatac gcatcaccga ccgtccgggg gcgcggcggg cggcagccgc
     2881 tttgaggagc cagaccgtga gattatgggc ggccatcagg cccacaacag tgaagatgat
     2941 tgtgatgtag acagatcgca cccagcgatt cacaagcgac cccgtgtctc gccctaggcc
     3001 gccgtctccg tgaatcctgc cgcgcgcgaa gttctcggtc gcgccgggat ggcatttccc
     3061 gcaggtttcg accaggcgat tgggatggat cgaggaatcg ggatcgtgag agggaagaat
     3121 gcggtggtat ccgtggcaac tggcgcaatt ggcggcgcgt gtcgaaccgt actgcacggc
     3181 caggccgtga tagctctgat agaaggtcct caccgggtcc ggtgcggggc gaaaactcgc
     3241 ggtgatcggc tcggctgcgt ggcatttgcc acagatttgg gccgagagcc ggcgcgcgga
     3301 agcgccatct gccccggcgc ccgcgtgctc gaaatgacaa tccgtgcata gggcggcatc
     3361 gcgctcgccc cggatggccg ccagaccgtg cacgctctcc gccacggcct gcgcggctcg
     3421 agggtgacac gcgccacaag ttgcgctgat cttcgagtga tgcagggtcg agtcggggga
     3481 tgtgtcgggc agcacctggt ggctgccgtg acagtcgctg catcgtgccg ctcgagcccc
     3541 gtctttggct gccgctccgt gcaggcgggc gccgtggctt gaggattggt cttcgtggca
     3601 cgcggcgcag tccacccgtt tggccgcaag attgtcgtcc ggatgctgcc tcgagatgtc
     3661 gccgtgacag gcggcacagg gcatggcccc atgcaccgag gcgccgtact tcgccccgtc
     3721 gacatgcaac gacacggtct gtcccgcctc gttaaccgca gtcaaatccg gatccccatg
     3781 acagtccatg cattcggaac cttgcatcga gtcagcagcc agggccatcg aggttgccca
     3841 cgctgccaag gccagacgac agaggagacg ggcgagtcgg ttcatcataa gcagttccgg
     3901 cgcgtgccct cgtgctcggt gatgcgtccg tggacgcgcg ggtgaaaccc gcacggtcgc
     3961 ggcgccgccc gccacgcccg acgggcggtc gcgatcgcgc tcagcggagc gtcctgcttg
     4021 gtccgtgcat cgtatcacat ggtgcggaca ctcgcggctg gacgaaaccg ggcgacgaga
     4081 ccagactgat tctcaccaga gttttgacta tgcctttcgc catctgcaac gagatatttg
     4141 gggattggcc gatcgaaacg gcctttcgct ttgccgctca gaccggttac gacgccatcg
     4201 agatcgcgcc gttcacgctc gcaaaccacg tgaccgatgt cccagccgcc cggcgcggcg
     4261 agattcgcga tgcagcggcc cgggccggga ttgcgattgc gggcattcat tgggttctgc
     4321 tcaagaccga aggcatgcac ttgacgcatt cggaacgtcc agtgcgggaa cgtacggcgc
     4381 gctacttcat cgatctggtt gatttctgcg ccgatctggg ggggcacatt attgtggttg
     4441 gctcccccaa acagcgcagt cttctgccgg gcgtgagctt cgagcaggct cgagagagcg
     4501 cggtcgaggt ttttcgcgat gcggtgcgcc gaggcgagga gcgcgaggtg accatctgct
     4561 tcgagcctct ggcgcccgcc gaaacggatt tcatcaacac ggccgccgag gcggtgtctc
     4621 ttgtgcaacg gttcgagtcg cctcgattca agatcatcct cgatgtgaag gcgatgtgct
     4681 cggaggcgcg ccctatcccc gacatcattc gcgaggcatg gccgcatttc gcccattttc
     4741 acgcaaatga tcgcaacctc aaaggcccag gcttcggcga tgtcgatttt cgtccgattg
     4801 ccgctgcgtt gcgcgaggtc ggatacgccg gatacgtctc ggtcgaggta ttcgatttca
     4861 cggagggtcc tgaagcgatc gcaacacgga gtctggcata cttacggcag gcgtttggga
     4921 atccgtatag cgaggcgcgc ctcgctcagt agaatccttg acccgtttaa cgcaacacag
     4981 aaactcgact tgtctgcaac aagtttcgcc attctggatc taacggcgtc cgggagccgg
     5041 cgttgtcgcg ggcggtctca gaggggcggg ggccttctcc gtcgagcgtg ggcgagagac
     5101 atccggagcg atcgggaaat gattcgtgag ttggctcacg ccgatgcgca aagcgaggcg
     5161 ccccagggca tataacacgt ccttttcggt gtcgaggttg gtatagattt cattctggac
     5221 accgagattt atggtttgga ttcggcccgg ggccgccagg ttgaggcgat gcaggcggcc
     5281 cagcgagaga ttgagcatgt ccaaaccccc gaacgtgaag tcgcttcgag gttcagacgc
     5341 ttgtttgcgg cgaagtcgcc ggtgaatcga ctcgaggttc gtctgccccg cgacatcttc
     5401 aactatgctg gcttccgcga gattcagtcg gaagaggcga agatgcaccc ggcgctgaag
     5461 cagcgccagc ctgtcatatt ccacatgaca ctcggccacg tgcaggaaca ccgaaccgcc
     5521 gaactcgggc ggattgtaga ggcggaggtt ctcgacagtg agcctcggtg agagaatgcc
     5581 gacttcgagc cgtccgatcc gcgtctccag gcctgtctcg cgctcgatat ggctggcaag
     5641 cagttccttc agcagcaggt ccttcagcag caccaccccg atcgagagga caagccccac
     5701 aaccagcagt cggaacagcc agcgaaaaac gaatcgcatg cagttctcgt atcacatcat
     5761 cgcgcgccct ggcgatactt aacaagagag tcctcacgac cgacagagtg ctcctgctcg
     5821 cctttctggg cgcggttccg ccaactagcc ccgtcgcggt tgctcgcgcg aagcgtgtga
     5881 aaaagtcggg ctagcagcct gtcggacttt tccccaacaa ttttgcccgg cgtttgaaca
     5941 a
//
LOCUS       DAOQRO010000225         5919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13611_length_5919_cov_5.101467, whole genome shotgun sequence.
ACCESSION   DAOQRO010000225 DAOQRO010000000
VERSION     DAOQRO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13611_length_5919_cov_5.101467"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..668)
                     /locus_tag="PKK20_09035"
     CDS             complement(96..668)
                     /locus_tag="PKK20_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV00069.1"
                     /translation="MSSNPTPPAGAPPGNATAAPGPATVELALELRQILVPLDFSAHA
                     MKALHYATGFARLSGAKLILLHVTEPIVYPSDFGYVPLPPNTLEENFQRDARDRLEAV
                     AAEQVKAGVTCEVALRLGKPYHEIAAAAREHQVDLVVITTHGYTGLTHVLLGSTAERV
                     VRHAPCPVLVVRYQETESAPAKTENAPAAG"
     gene            complement(950..4078)
                     /locus_tag="PKK20_09040"
     CDS             complement(950..4078)
                     /locus_tag="PKK20_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV00070.1"
                     /translation="MRARIAHGPRALRAARAILVVLCLRILLGCVGSAEVPRESDWRI
                     YKAADGLTESITTAITVTPRGNLWVKHGEVDAVSFLDGYVIRHIPAPGERNYRIYESR
                     AGRIWSVYADGLMEFVSDEWIRHPIPEIRLEGEANLARSLRQVPLLPAEQDRVLILLP
                     DRLAEYRATARELQVLRWARDTRLGRFIDMLPTQDGAVWITGTAGAARLPGPLRQLSA
                     QTPWAEHLLPPAWPIRDVQRPFEDDAGGITGLGESTALARRVLVHFDGTNWLWRAFPE
                     GSLRYAWRDLEPGHFWGLTINSLVRLSVDTDAIERIDLPPNQYYDAAVQPRGVFWLAT
                     LDGLMRYAPPAWRVPPGAAAFDSIVHAGVEDNQGRLWFATGSGLLVNRDGQWQQYSWP
                     EGFEPLFRARDGLFLVGDNQIAIGVAEQLWIFTPDTGAMKPIEHPSGRRIRKVLRQWS
                     SSTLCLQTAEPDAPPTAYQFELFDGRSFREWTDAPAAVDLGSELFFLAVAQNGDLWLG
                     GSSGAALWHDGRWQRFTSADGYAAEGALCWTELSDGRIWCAGLGRIVEFDGKVWTTVL
                     SSLDRVHAMIRARDGTVWVATASSLYRQYKESWAWLSQDDGLPADACFTLFEDRSNRL
                     WVGTSRGIVHYSPRADIDPPRTLSVVIEQSGNGRPDSTIEVQIRGRDRWQQTPDHRLL
                     FSHRLNGGNWSPFASGPMIALRNVPPGKHRLEVRTLDRNWNFEPKPFLAEFQVVVPWY
                     REGRILFAVGVGTVVALFFAALAVNRHLQLRRSYAEVELKVAERTRELEQATQALVHS
                     QKMNALGTLAAGIAHDFNNILSIIRGSAQIIEVNLDDRDKVLTRVARIKTMVDQAGGI
                     VRAMLGFGRVGDTGLHACKPAEVIDQTIRLLSDRLRRTVHIERSVASNLPAIRAARDL
                     TQQMLVNLILNASDALPPGGEIRIRARPLDMLPHNPILPPQPASGYVAISIEDNGQGI
                     PSDVLSRIFEPFFTTKAFSSRRGTGLGLYMVYEFAREMGHGLIVTSEAGRGSQFTIIA
                     PIADNPDATPVP"
     gene            complement(4234..>5919)
                     /locus_tag="PKK20_09045"
     CDS             complement(4234..>5919)
                     /locus_tag="PKK20_09045"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV00071.1"
                     /translation="LATNWRDYHKGLQEGKPLFYRDGAGATVSVHTGENPETDFYLKV
                     NAKTDASTLGDMTTQLLSGHLPLLLHPESTNVLVIGLGSGVTAGAIAAHSSVQTIDVV
                     EISPEVVEASRLFNPYNRDVFRDPRVRLVIDDAKSYLLLNPRRYQTIISEPSNPWMAG
                     VAGVFTLEFYEECRAHLDPGGVMVQWVQLYEFSDTGLETVVATFSQVFPHVSIWHTDA
                     ADILLIGTVEPRSSDLAAFAARLDEPSVKADLERIKLTNMPLLLSHELISSAHGALLP
                     PIETRIHSDYYPTLEYLAQRDFFVRHGATRFRQIDENFSRRPTTLLGDYLRANALTWA
                     DFEAFANIYLESGKPFLDIITSVLLRWQQQFPEAWEPLEVASRFVFVNAPGELDVLRL
                     APLRDGLIDRARVKPDLLRQYGLALMRTYSEQRSAFYAPPTEELTAILDRLIELDPDN
                     QRVYQVYLAELAWDRGDDDLCLDYGRRAMTADLPRGSAKFKLDPVAPLRVVARLADVM
                     RRRGELVQAAELCETAVRGGYLTDSARAGALIFEVTQRRVQAELNQLATRRAL"
BASE COUNT         1023 a         1679 c         2149 g         1068 t
ORIGIN      
        1 aatcggcaga cgcgctagac tcaaaatctg gtactcgaaa gagtgtgtgg gttcgacccc
       61 ctccgccggc accaatcccc gttcaagaaa attctctatc cagccgccgg ggcgttttcg
      121 gttttagcgg gggcggactc ggtttcctga tagcgaacga ccaggacagg gcagggggca
      181 tggcggacga ctcgttccgc ggtgctgccc agaaggacat gggtcaggcc ggtgtagccg
      241 tgggtggtga tcacgaccag atcgacctgg tgctcgcggg ccgcggcggc gatttcatgg
      301 tagggtttgc ccaggcgcag agcgacctcg caggtcacac cggccttgac ttgctcggca
      361 gccacggcct cgagacgatc ccgcgcatcg cgctggaagt tctcttcgag cgtgttcggg
      421 ggaaggggga cgtaaccgaa gtcggacgga tagacgatcg gttcggtgac atgcagcaga
      481 atgagtttgg cgccggagag tcgggcaaaa ccggttgcgt aatggagggc tttcatcgcg
      541 tgcgccgaga aatccagcgg cacgaggatt tgccggagtt cgagggccag ttccacggtg
      601 gcgggaccgg gtgcggcagt tgcgttgccg ggtggcgcac cggcaggggg cgtcgggttt
      661 gagctcatat gcagcgatcg gttgtgtggt ccaatttccg ggtgattgta cgcgatcggg
      721 ggcgcgaact caacccgtag tgagcggcct gcagcggttg cgcggcgcca tctcatgaac
      781 tggccctgtt tgttgcgcgc ctgcgctcca ggcccggccg gcgtgttgtc caggtagaga
      841 gagtctcagg cgcttttccg gacaaacacg gatggacaaa cagatcagtc acacgccagg
      901 ttcgacagat cgagaactgc aacacattgt tcccattttt gcacagaccc taaggaacgg
      961 gcgtggcgtc cggattgtcg gcgatcgggg cgatgatggt gaactggctg ccgcgtccgg
     1021 cttcggaagt gacgatcaga ccgtgtccca tttccctggc gaactcgtag accatgtaga
     1081 gtcctaggcc cgtgccgcgg cgggatgaaa aggccttggt tgtgaagaac ggttcgaaga
     1141 tgcgggacag aacgtcgctt ggaatcccct ggccattatc ctcgatgctg atcgcgacgt
     1201 aaccggaggc gggctggggc ggcagaatgg gattgtgggg gagcatgtcc aagggccggg
     1261 cccggatgcg aatctcgccg ccggggggaa gggcgtctga ggcgttgagg atcaggttga
     1321 cgagcatctg ttgcgtcaag tcgcgcgcgg cgcggatggc aggcaggttt gaggcgacgg
     1381 agcgttcgat gtggacggtg cggcggaggc ggtcgctgag aaggcggatg gtttgatcga
     1441 tgacttcggc gggcttgcag gcgtggaggc cggtatcgcc gacgcgtccg aatccgagca
     1501 tggcgcgaac gatgccgccg gcctgatcga ccatggtttt gatgcgggcg acgcgggtga
     1561 ggaccttatc gcggtcgtcg aggttgacct cgatgatctg ggcggagccg cgaatgatcg
     1621 agaggatgtt gttgaagtca tgggcgatcc cggcggcgag ggtgcccagg gcgttcattt
     1681 tctggctatg gacaagcgcc tgggtggcct gctcgagttc gcgcgttcgt tccgcgactt
     1741 tcaattccac ctcggcatag ctgcggcgga gctggaggtg gcggttgacg gcgagcgcgg
     1801 cgaagaacag tgccacgacg gtcccgacgc ccacggcgaa gagaatccgc ccttcccggt
     1861 accagggcac gacgacctgg aactcggcga gaaaaggctt gggctcaaaa ttccagttcc
     1921 ggtcgagggt tcgcacttcg agtcggtgtt tgccgggcgg cacgttgcgc agggcgatca
     1981 tgggtccgga tgcgaacggc gaccagttgc ccccattgag cctgtgggag aagaggaggc
     2041 ggtggtcggg cgtttgctgc catcggtcgc gtccccggat ctggacctcg atggtgctgt
     2101 cgggccgtcc attgcccgat tgctcgatga caacggagag agtgcgaggc gggtcgatgt
     2161 cggcgcgcgg ggagtagtgg acaatgccgc gcgaggttcc gacccagagt cggttcgagc
     2221 ggtcttcgaa cagggtgaag caggcatcgg cgggcaagcc atcatcctgg ctcaaccagg
     2281 cccagctttc cttgtactgc cggtagaggc tgctggcggt ggcgacccag accgtgccgt
     2341 cgcgggcgcg gatcatggcg tgaacgcggt cgaggctgct cagcacggtg gtccagacct
     2401 tgccatcaaa ctcgactatc cgacccagcc cggcgcacca gatgcgccca tcgctcagct
     2461 ccgtccagca gagcgcgccc tcggcggcgt agccgtcggc ggacgtgaac cgctgccagc
     2521 gtccgtcgtg ccagagggcg gcgccgctgc tgccgcccag ccatagatcg ccgttttggg
     2581 cgacggcgag aaagaagagc tcgctcccga gatcgactgc ggcaggcgca tcggtccact
     2641 cgcggaagct gcgtccatcg aacagttcga actggtatgc ggtcggaggg gcgtccggct
     2701 cggcggtctg gaggcagaga gtcgagctgg accattggcg caggaccttg cggatgcggc
     2761 gtccggacgg atgctcgatg ggcttcatcg cgcccgtgtc gggcgtgaaa atccagagtt
     2821 gctcggccac gccgatagcg atctggttgt caccgaccaa gaagagtccg tcgcgtgcgc
     2881 ggaacagggg ttcgaagccc tcaggccagg agtattgctg ccactggccg tcccggttga
     2941 ccagcagacc cgagccggtg gcgaaccaga ggcgtccctg gttgtcttcg acgcctgcat
     3001 ggacgatcga atcgaatgcg gcggcgccgg gagggacgcg ccacgctggc ggggcataac
     3061 gcatcagacc atcgagcgtg gcgagccaga acacgcctcg cggctggacg gcggcgtcgt
     3121 agtactgatt gggcgggaga tcgatccgct cgatggcgtc agtgtccacg ctgagtcgga
     3181 ccagggagtt aatggtcagg ccccagaagt gtccgggctc gagatcgcgc caggcgtagc
     3241 gaaggcttcc ttcagggaac gcccgccata gccagttcgt gccgtcgaaa tgcaccagga
     3301 cccgtcgggc cagggctgtg gattcgccca ggcccgtgat gccgccggca tcgtcttcga
     3361 acgggcgctg gacatcccgg attggccagg ccggagggag cagatgctcg gcccagggtg
     3421 tctgggcgct gagttggcgg agcggacccg gcagtctcgc ggcgccggcg gtgccggtga
     3481 tccagaccgc gccgtcctgg gtgggcagca tgtcgatgaa ccggcccagg cgggtgtcgc
     3541 gggcccagcg cagaacctgc aactcgcgcg ccgtggcacg atactcggcc aggcgatcgg
     3601 gcaggaggat gaggacgcgg tcttgttcgg cgggcaggag cgggacttgg cgaagcgagc
     3661 gggccaggtt ggcttctcct tcgagccgga tttcggggat cggatgccga atccactcgt
     3721 cgctgacgaa ttccatcagg ccgtctgcat agaccgacca gattcgcccg gcgcggcttt
     3781 catagatgcg gtagttgcgt tcgccggggg cggggatatg gcggatgaca tagccatcga
     3841 ggaagctcac ggcgtcgacc tccccatgct tgacccagag attgccgcgg ggcgtcacgg
     3901 tgatcgcggt ggtgatggat tccgtgaggc catcggcggc cttgtagatg cgccaatcgg
     3961 attcgcgcgg cacttcagcg gagccgacac aaccgaggag gatgcggagg cagaggacga
     4021 cgaggatcgc gcgcgccgct cgcagcgcgc ggggaccgtg cgcaatccta gcccgcatag
     4081 cagtcttttt gcgaagtccg agtgccctgt gcaccatcgc ggtgtaccac tctctgtcaa
     4141 ctttctttct ttttgaagga agtatcgtcc cgcaaaggcc gtcagccgct gcacattctg
     4201 gttgcggctt tgccgcgctg cggaatatgc ggactagagg gcgcggcgag tcgccagttg
     4261 gttcagctcg gcctgcacgc ggcgttgggt cacttcaaaa atcaatgcgc cagcccgggc
     4321 gctgtccgtg agatagcctc cgcgcaccgc ggtctcgcag agttccgccg cctggacgag
     4381 ttcgccgcgg cggcgcatga catcggccag gcgagccacg acgcgcagag gcgccacggg
     4441 atcgagcttg aacttcgccg agccgcgggg cagatcggct gtcatggcgc ggcgtccgta
     4501 gtcgaggcag aggtcgtcat cgccgcggtc ccaggccagc tcggccaggt agacctggta
     4561 gacgcgctga ttgtcgggat cgagttcgat gaggcgatcg aggatggcgg tcaactcctc
     4621 cgtcggcggg gcatagaacg cggagcgctg ttcgctgtag gtgcgcatca gcgccagacc
     4681 gtactgccgc aggagatcgg gcttcacgcg ggccctgtcg atgagcccat cccggagggg
     4741 ggcgagccgg aggacatcca attcgcccgg ggcgttgacg aagacgaagc gtgaggcgac
     4801 ttcgagcggt tcccaggcct cggggaactg ctgctgccag cggaggagca cggaggtgat
     4861 gatatcgagg aacggtttgc cgctttcgag atagatattg gcgaaggcct cgaagtccgc
     4921 ccacgtgagc gcattggcgc ggaggtagtc gcccagcagc gtggtcgggc ggcgcgagaa
     4981 attctcgtcg atctgccgga atcgggttgc gccatggcgg acgaagaaat cgcgttgggc
     5041 caggtattcg agcgtcgggt agtagtcgct atgaatgcgt gtttcaatgg gcgggagcag
     5101 ggcgccgtgt gcggatgaga tcagctcgtg cgaaagcagc agcggcatgt tggtgagttt
     5161 gatgcgttcg agatcggcct tcaccgacgg ttcgtcgagg cgcgccgcga aggcggccag
     5221 gtcgctcgaa cgcggttcca cggtgccgat gagcaggatg tccgcggcgt cggtgtgcca
     5281 gatgctgaca tgtgggaaga cctggctgaa tgtggcgacg actgtttcga gtccggtgtc
     5341 gctgaattcg tagagttgga cccactggac catgacgccg ccggggtcga ggtgggcgcg
     5401 gcactcttcg tagaattcga gtgtgaagac gcctgccacg cccgccatcc atgggttcga
     5461 gggctcgctg atgatcgttt ggtagcgccg cggattgagc aggaggtagg acttggcgtc
     5521 gtcgatgacc agccgcacgc gcgggtcgcg gaagacatcg cggttgtagg ggttgaacag
     5581 cctcgaggcc tcgaccactt cgggcgagat ctcgaccaca tcgatggtct ggacgctcga
     5641 atgggcggcg atcgccccgg ccgtcacgcc gctgcccaag ccgatcacga gcacattcgt
     5701 cgattcggga tggagcagca gcggcaggtg cccggacagc agttgggtcg tcatgtctcc
     5761 gagcgtgctg gcgtcggtct tggcgttgac cttcaggtag aagtccgttt ccgggttttc
     5821 gcccgtatgg acgctgacgg tggcgccggc gccgtcgcga tagaacagcg gtttcccttc
     5881 ctgcagccct ttgtgataat cgcgccagtt ggtggcgag
//
LOCUS       DAOQRO010000226         5892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13703_length_5892_cov_5.330821, whole genome shotgun sequence.
ACCESSION   DAOQRO010000226 DAOQRO010000000
VERSION     DAOQRO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13703_length_5892_cov_5.330821"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..469
                     /locus_tag="PKK20_09050"
     CDS             110..469
                     /locus_tag="PKK20_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNV00072.1"
                     /translation="MIRGNVENVAACQDALRRWLARRCHQALVPWLREVSREKHLPFG
                     RTLVKMQKTRWASWSQHRTMSLNMKLLFLPPELVRYVLVHELCHTVHMNHSREFWRFL
                     AANEPGYIELDRWTRVS"
     gene            complement(510..1463)
                     /locus_tag="PKK20_09055"
     CDS             complement(510..1463)
                     /locus_tag="PKK20_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNV00073.1"
                     /translation="MKITVTLADAILLSALSAGLLEAANPVLPGADPHAVAVGDTVWI
                     YPTWSDRPFERFFAFSSTNLIAWQRHGPVLDFEDVAWIKDDRQARHHAWAPSAIEKNG
                     TWFFYYSVGPQNPTPSRIGVAVGAGPQGPFRDSGKPLLTGGGGFEAIDPMVFTDPRSG
                     KSFLYAGGSAGATLRVFELNPDMISFAREIAVETPPQFTEGAFMHCRQGRYYLSYSHG
                     GWQHASYSVHYVTADGPVGPWHYRGAILMSDATRKGPGHHSFLQRPGTEDWLIVYHRW
                     ENQTGDGPYRGRRQICIDHIEYTPDGLIQPVIMTGEEPPRP"
     gene            complement(1574..2668)
                     /locus_tag="PKK20_09060"
     CDS             complement(1574..2668)
                     /locus_tag="PKK20_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HNV00074.1"
                     /translation="MRAPSRHASGGFAIIVVLIVIFTLAILAGSFALAMKVESRLAAN
                     ANSDAELEWLGRSGVELARYVLGQSMGQPYASLNQIWAGGPGSGNETNGPLMDISLTD
                     VQLGEGRFSVRIEDLERKLNINLNLPEQEKKQVLARALTTIIGVDSGDSATIEDSILD
                     WLDPDDASHLSGTESDFYLTMPRPYFAKNGPIDDLAELLLVRGVTPEIYWGPSAPHHQ
                     VQLLKPSAGAIESDVLPMYPVGLVDLFTALSSGRININTASANVLRLLPGVDENVAGN
                     IIRARAGPDGVEGTEDDTPFVNLAGLNPAMVPGIIPELAARYNMLCGVTSGTFEVLVD
                     ARLGRHRRTYAAIVRRNRPDDVAVLQFGWK"
     gene            complement(2671..3477)
                     /locus_tag="PKK20_09065"
     CDS             complement(2671..3477)
                     /locus_tag="PKK20_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV00075.1"
                     /translation="MTTRRTNRGFTLLEIMLAVMIFGMVVTAIYSSWSAILRASRAGR
                     EASEDMQRSRIAARTIEDALVSTVMFTSNPGLYSFLADTSGDFAALSFVSRLPPSFPG
                     SGYFGDQIVRRVEFTAEPSPSGELQLLLRQMPILQTNVFDPDEHTIVLARDLSAFMIE
                     FCYQRGNSYEWVEEWHLTNQLPKLVRFALAFGKSPNGTGKPKSVTVRAVSLPSAAVPR
                     DSQGAVGPGTPAPPQDGPVNQPANDQAQRPNIDQRFGAPVRGIGGGGGGL"
     gene            complement(3474..3929)
                     /locus_tag="PKK20_09070"
     CDS             complement(3474..3929)
                     /locus_tag="PKK20_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV00076.1"
                     /translation="MKRLPSPPRAPLAPDPQARSAWGAAGLSGRGFTLLEVMIAMAIF
                     FGAIFAILDLVTQNLRIARGLSLGEIDFSSVASELSLTNRLEEGTASGDFGDSKPGAS
                     WIAETMLYASNGLYQIDITVDWPENGLVKQRTASILLYRPASRNVRGGR"
     gene            complement(3926..4498)
                     /locus_tag="PKK20_09075"
     CDS             complement(3926..4498)
                     /locus_tag="PKK20_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00077.1"
                     /translation="MNAAGTNLRTRGFTLFEILVVLAILSIVATISVPSVVQAAKKSP
                     MRQAMSDLEEACRNARMLAILQGEPMEIVIRAGEGSFTVSRAFSAPPPTAESGAVEGN
                     AYAPVESQPARATPPEVEPFRARIPASVAFKRLLVNLQDMMDAHEARVRFYPNGTCDA
                     FTAHLLSEQNEERSLTLEITTGREIVEVIR"
     gene            complement(4498..5772)
                     /locus_tag="PKK20_09080"
     CDS             complement(4498..5772)
                     /locus_tag="PKK20_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV00078.1"
                     /translation="MPQFTYKARRRSGELVQDVVEANDRPSALVQIERLGLFPVMVES
                     ARGSGAGRAERSAGRPRPSAGLLPPALRELLQRKRRPKLQELATFTQQLTNLLRAGMP
                     LASALNSMSYLGTKGIPPEVSRQLKQDVMEGKGLSDGMAKQPVIFSDLYINMVRAGEQ
                     SGALSDVLQRMATHFERFAEVQHKFTSALIYPAVVCCVGVLIMVFFMTFMLPRFMTIF
                     DGMKMELPAATKFLVGLSRFFSGYWWTIPLVAAALLIIYNRYKATDAGRRAIDRFKMN
                     APVFGKVVRLNLFGQFARTLGTLLQNGVPVLTALKITEQVMPNRMLREAIAHTREEVT
                     DGKTIAQPLARSKLFPQLMIDLIRIGEETGDVPGALNNVAQTYENELSIALRVMTNLI
                     EPAMIIVMALGVGFLLFSVLSAMFAITANIAR"
BASE COUNT         1076 a         1830 c         1939 g         1047 t
ORIGIN      
        1 ccaggggcgc gccgggcacc tccaggaaac gggtcagcgg ctcccagcgt ttgagcatat
       61 aggtgagggc ctgtgggcgg gagtgtaccg ccatggcgga gatcgggtag tgattcgagg
      121 aaacgtcgag aacgtggcgg cgtgccagga tgccctgcgc cgttggctgg cccggcgctg
      181 ccatcaagcc ttggtgccct ggctgcgaga agtcagccgt gagaagcatc tcccatttgg
      241 ccggacactc gtgaagatgc aaaagacgcg gtgggccagt tggtcgcagc acaggacgat
      301 gagcctgaac atgaagctgt tgttcctgcc gcccgaactg gtgcgctacg ttcttgtcca
      361 tgaactctgc cacaccgtcc acatgaatca ctcccgcgag ttttggcggt tcctggcggc
      421 gaatgagccg ggctacatcg aattggatag gtggacgagg gtgagttgat cggtcatggc
      481 gtgaccgcat gggtcttgaa agacgcgggc tatggccgag gcggctcttc accggtcatg
      541 atgaccggct gaatgaggcc gtcgggggta tattcgatgt ggtcgatgca gatctggcgt
      601 cggccgcgat agggaccgtc gccggtctgg ttctcccagc ggtggtacac gatcagccag
      661 tcctccgtgc ccggccgctg caggaaggag tgatgcccgg gccctttgcg ggtcgcatcg
      721 ctcatcagga tggcgccgcg atagtgccac ggtccgacgg gtccatcggc cgttacgtaa
      781 tgcaccgagt aagaggcgtg ctgccatccg ccgtggctgt aggacagata gtaacgcccc
      841 tggcggcaat gcatgaaggc gccttcggtg aactgcggcg gagtttcgac ggcgatctcg
      901 cgcgcgaaac tgatcatgtc ggggttcaac tcgaacacgc gcagtgtggc gccggcgctg
      961 ccgcccgcat agaggaagga ctttcccgag cgcggatcgg tgaacaccat cgggtcaatc
     1021 gcctcgaacc cgcccccgcc cgtcagcagg ggtttgcccg aatcgcggaa cgggccttgg
     1081 gggccggcgc ccacggcgac cccgatccgg gaaggcgtcg ggttctgggg gccgaccgag
     1141 taatagaaga accaggtccc gttcttctcg atggcggacg gcgcccaggc atgatggcgc
     1201 gcctgacggt cgtctttgat ccaggccaca tcctcgaaat cgagcacggg tccgtggcgt
     1261 tgccaggcaa tgaggttggt cgaggagaag gcgaaaaaac gctcgaacgg gcgatcgctc
     1321 caggtcgggt agatccagac cgtgtcgccc acggccacgg catgcgggtc agcaccgggc
     1381 aggacggggt tggcggcttc gagaagcccc gcagacaacg cgctcagcag gatggcatcg
     1441 gccagagtaa cggtgatctt catcggtttc atagggtttg cgcccgagcg ccccggcggg
     1501 ccttcagcga tcggatctga tctcggacac tttcgtttcc ggactgcgcc tggttcgatg
     1561 tctgcatggc cgactatttc cagccgaact ggagaacggc gacatcgtcg ggccggttgc
     1621 ggcggacgat ggcggcgtag gtccggcggt ggcgtcccaa ccgcgcgtcg accagcactt
     1681 caaacgtgcc gctggtgacg ccgcagagca tgttgtatcg ggcggccagc tcgggaatga
     1741 tgcccggcac catcgccggg ttgaggccgg cgaggttcac gaagggcgtg tcatcctcgg
     1801 ttccctcgac gccatcggga cccgcccgcg cgcggatgat attgccggcg acattctcgt
     1861 cgacgcctgg cagcaggcgg aggacgttgg cggacgcggt attgatgttg atgcgtccgc
     1921 tcgagagcgc agtgaacaga tcgaccaatc cgaccgggta catgggcagc acgtccgact
     1981 cgatcgcacc cgcgctgggc ttcaacaact ggacctggtg atgaggcgcg ctgggccccc
     2041 agtagatttc cggggtgaca ccccgcacca ggagcaactc cgccaggtca tcgatcggcc
     2101 cgttcttggc gaaatagggg cgcggcatcg tgaggtagaa atcgctctcg gtcccgctca
     2161 gatgcgatgc gtcgtccgga tcgagccaat cgagaatcga gtcctcgatc gtcgccgaat
     2221 cgccgctgtc gaccccgata atggtggtga gcgcgcgggc gagcacctgt tttttctcct
     2281 gctcgggcag gttcaggttg atgttcagtt tacgttcgag atcttcgatg cgcaccgaga
     2341 accgcccctc gccgagctgg acgtccgtga ggctgatatc catgagcggg ccgttggtct
     2401 cgttgccgct gcccgggccg cccgcccaaa tctggttgag cgaggcgtac ggctggccca
     2461 tcgattgccc gaggacgtag cgcgccagtt ccacccccga tcgccccaac cattcgagtt
     2521 cggcatcgct gttggcgttg gccgccagac gggattcgac cttcatggcc agggcgaagc
     2581 ttccggccag aatggccagc gtgaagatga cgatcaaaac gacaatgatc gcgaatccgc
     2641 cggaggcgtg tcgggacggc gcgcgcacgg tcacaaacca ccccctcccc cgccgatccc
     2701 gcgcaccggc gctccgaacc gctggtcgat gtttgggcgt tgtgcctggt cattggcggg
     2761 ttggttcacg gggccgtcct gcggcggcgc gggtgttccg gggccgacgg cgccttgcga
     2821 atcgcgcggc accgcggcgg agggcaggct cacggcgcgc accgtaacgc tcttgggttt
     2881 gccggtgccg ttgggcgact ttccgaaggc cagggcgaag cgcacgagct tgggcagttg
     2941 gttggtcaag tgccactcct cgacccattc gtaggaattg ccgcgctgat agcagaactc
     3001 gatcatgaag gcgctcagat cgcgcgccag aacgatcgtg tgttcgtcgg gatcgaacac
     3061 gttcgtctgg agaataggca tctggcggag gagcaactgc agttcgccgc tcggggaagg
     3121 ctcggcggtg aactcgacgc gccgcacgat ctgatcgcca aaatagccgc tgcccggaaa
     3181 ggaaggcggc agccgggaca cgaagctgag ggccgcaaaa tcaccgctgg tgtccgccag
     3241 aaaggaatac aaccccggat tgctggtgaa catgacggtg gacaccaggg cgtcctcgat
     3301 cgtgcgggcg gcgattcggc tgcgctgcat gtcctccgag gcttcgcgcc cagccctcga
     3361 ggcacggaga atggccgacc aactcgagta gatggccgtg acgaccatgc caaaaatcat
     3421 cacggccagc atgatctcga ggagggtgaa cccccggttt gtcctcctgg tcgtcatcgt
     3481 cccccccgca cgttgcgcga cgccggccgg tataggagaa tgctcgccgt gcgctgcttc
     3541 accaggccgt tctccggcca atccaccgtg atgtcgatct ggtacaaccc gttggacgca
     3601 tagagcatcg tctccgcaat ccaggaggcc cccggcttcg aatccccaaa gtcgcccgac
     3661 gccgtccctt cctcgagacg gttggtcagt gagagctccg acgccacgga actgaaatcg
     3721 atctccccaa ggctcaatcc ccgcgcgatg cgcaggttct gggtcactaa atccagaatg
     3781 gcgaaaatcg cgccgaagaa aatcgccatc gcgatcatca cctcgagcag tgtgaatccg
     3841 cgtccggaca atcctgccgc gccccatgcg gatcgggcct ggggatcggg agccaacggc
     3901 gccctgggcg gggaaggcag acgtttcatc ggatcacctc gacgatctct cggccggtgg
     3961 tgatttcgag cgtgagactg cgctcctcgt tctgttcgga gagcaggtgc gccgtgaatg
     4021 catcgcaggt tccgttgggg tagaaccgaa cacgcgcttc gtgggcgtcc atcatgtcct
     4081 ggagattcac caggagtctt ttgaaggcca cactcgccgg aatccgggcc cggaacggct
     4141 cgacctcggg gggcgtggcc cgggccggct gggactcgac cggggcatac gcattgcctt
     4201 cgacggcgcc cgattcggcc gttgggggcg gggccgagaa ggcgcgggac acggtgaagg
     4261 agccttcccc ggcgcgaatc acgatctcca tcggctcgcc ctggaggatc gccagcatgc
     4321 gggcgtttcg gcaggcctct tcgagatcgc tcatcgcctg gcgcatcggg cttttcttgg
     4381 ctgcctggac gacggacggg acgctgatgg tggcgacgat gctgaggatg gcgaggacga
     4441 ccaggatttc gaagagggtg aatcccctcg tccgcaagtt tgtcccggca gcgttcatca
     4501 gcgggcgata ttggcggtga tcgcgaacat ggcggagagg acgctgaaca gcaggaagcc
     4561 gacgcccagg gccatgacga tgatcatcgc cggctcgatg agattcgtca tgacgcgcag
     4621 ggcgatgctc aactcgttct cgtaagtctg ggccacgttg ttcagcgcgc ccggcacatc
     4681 gcctgtttcc tcgccaatcc ggatcaaatc gatcatcaac tggggaaaca gtttgctgcg
     4741 cgccagcggc tgcgcgatcg tcttgccgtc ggtgacctct tcgcgcgtgt gggcgatggc
     4801 ttcacgcagc atgcggttgg gcatgacctg ctccgtgatc ttcaaggcgg tcagcaccgg
     4861 cacgccattc tgcagaagcg tgcccagagt ccgcgcgaac tggccgaaca gattgaggcg
     4921 aaccactttc ccgaagaccg gcgcgttcat cttgaaccgg tcgatcgccc ggcggcccgc
     4981 atcggtcgcc ttgtagcggt tgtagatgat cagcagtgcc gccgccacca ggggaatagt
     5041 ccaccaatac ccggaaaaga aacgactcag gccgaccagg aacttcgttg ccgccggcag
     5101 ctccatcttc atgccgtcga agatggtcat gaagcggggc agcatgaagg tcatgaagaa
     5161 gaccatgatc agcacgccca cgcagcagac gaccgccggg tagatgagag cggatgtgaa
     5221 cttgtgctgg acctcggcga agcgctcgaa atgggtggcc atgcgctgca gcacatcgct
     5281 cagcgcgccg ctctgctcgc cggcgcggac catgttgatg tataggtccg agaagatgac
     5341 gggctgcttg gccatgccgt cggaaaggcc cttgccttcc atcacgtcct gcttgagctg
     5401 ccggctgacc tcgggcggga ttcctttggt gcccaggtag ctcatgctgt tcaaggccga
     5461 cgccagaggc atgccggccc gaagcaggtt ggtcaactgc tgcgtaaatg tcgccaactc
     5521 ctgcagcttc ggacgccgct tgcgctgaag caactcccgc aacgccggag gcagcagccc
     5581 cgccgatgga cgcggccggc ccgccgatcg ctcggcccgc cccgccccgc tcccccgcgc
     5641 cgactcgacc atgacgggga acaagcccag acgctcgatc tgaaccaggg cactgggacg
     5701 atcattcgcc tcgaccacgt cctggaccag ttcgcccgac cgccggcgag ccttgtaagt
     5761 aaactgcggc atactaataa cactctttga tcaccgcgct gggcctaaag tttccgcatc
     5821 ccccgcaaaa cgcaagactt gtagcccgcc agcccgcatg tttcacaccc cgacggtgca
     5881 cagggcggtc ag
//
LOCUS       DAOQRO010000227         5887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13722_length_5887_cov_3.963477, whole genome shotgun sequence.
ACCESSION   DAOQRO010000227 DAOQRO010000000
VERSION     DAOQRO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13722_length_5887_cov_3.963477"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(504..1349)
                     /locus_tag="PKK20_09085"
     CDS             complement(504..1349)
                     /locus_tag="PKK20_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00079.1"
                     /translation="MTNMDWPFASARPAASHRTRAGFTLIELLVVIAIIAILASMLLP
                     ALSRAKAKATAISCANNLKQLGVIWTMYAGDNNEALVLNGSGTEATVPLTWVGGSFAT
                     AESDITNIFKLIDPKYSLFGPYLRSTDIYRCPSDKTLERVGNKKVPVVRSYGMNSMVG
                     WKGPAYRDNPPSGYKVYTRSGDFTEVGPSEIFVFSEIHHNSICRPFFGMIMTRPAFYH
                     VPANYHKPSSTFAFADAHVEIHKWVDSRTYNPPRGLSWHSHDYPVPNSRDAVWLQQHA
                     SVRIR"
     gene            complement(1476..2180)
                     /locus_tag="PKK20_09090"
     CDS             complement(1476..2180)
                     /locus_tag="PKK20_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV00080.1"
                     /translation="MSNDDPHASGPALVAGRDLHKAYRMGSRAVEVLRGVSLEVPRGA
                     FLALRGASGAGKSTLLHLLGGLDTPTAGDVLVDGRSLAVMSAKELAVFRNRRIGFVFQ
                     AYHLLPELDALENVGLPARIARVPATEASRRAAQLLRRVGLEHRLDHRPGELSGGEQQ
                     RVAIARALVNAPNLILADEPTGNLDTHTGDGIIDLLCDLQAEHQATLIIATHDSRVAA
                     RVPHAIELEDGRLISG"
     gene            complement(2173..3444)
                     /locus_tag="PKK20_09095"
     CDS             complement(2173..3444)
                     /locus_tag="PKK20_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV00081.1"
                     /translation="MSRLPFEFMLALRYLRPKRTFVSAITLISIVGVLLGVAVLIIVI
                     AVMSGFDQQLREKILGFNAHLRIEDSDVPLHDYERLMELVAAHPFVRGVAPYVEGQVM
                     IKTQPEVGSPRYIVPLVRGVDPRFEPRISRLLDSLWAGTNDLRGYSLLVGRQLAANLG
                     LRLGDTVAIYAVRQFEKWDSARKQGEEEAPVADDYTVRGIFEVGFHEFDYAYLVTSLA
                     NAQDFFDLHNEAHGLMVALRDPDQAPLAKAELQADLGGRYVIRTWMEENSTILDALVV
                     EKQMMFYLLFFITIVAAFGICSGLITFVVQKTREIGTLKAIGASAGQVMALFLGQSLV
                     VGVLGVTAGLGLGLLAVAYRNDFLSFMRRVTGFDLFPAQIYYFNELPALILPQDLAVI
                     CGGSLIICVLAGLIPAWNAGRLKPVEALRHE"
     gene            3547..4227
                     /locus_tag="PKK20_09100"
     CDS             3547..4227
                     /locus_tag="PKK20_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNV00082.1"
                     /translation="MTDQPYGFAREDPWRIFRIMAEFVDSFETLAKLRPAVTIFGSAR
                     TSPGDKYYKLAETLARELGKHRLALITGGGPGIMEAANKGAAESKAHSVGLNIELPFE
                     QKGNRFANVPLHFHYFFSRKVCFVKYSIAFVFMPGGFGTLDEFFEVITLVQTQRIPRF
                     PLILFGRHYWNGLLRWCKRTVEAPGFISPGDLDLVTLTEDPHEAVEIILDYLRRVGPP
                     ESIPKAFC"
     gene            4473..5774
                     /locus_tag="PKK20_09105"
     CDS             4473..5774
                     /locus_tag="PKK20_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV00083.1"
                     /translation="MKRRTGFDLRRASFVLLDNGIRVALAPMPHMASVSLGFWIGVGG
                     RHEPPSLNGISHFIEHMLFKGTRRRSARQISEAVEGIGGYLNAFTSEEHTCFYSKARH
                     DRFPELLDVLTDMLLHSRFAPDDIETERDVIRDEIAMYLDQPQQHVQELLNAVSWPRH
                     PLGRPLTGTKRTVSTLTRRQLQAYHRQHYVGAATVIAVAGHFNLQPTLRTIRRLTRGW
                     PTGQPDVFPPVTARNRGPAVRLATRRIEQTHLALGIRTCSRHDERRFALRLLNVILGE
                     NMSSRLFQRIREDAGLAYSIYSSVSFFADTGDLVISTGLDTEHLDRVLRLIVAELRRL
                     VVEMPGRAEFHRARDYALGQIDLGLENTENHMLSVGEQVLGYGRPVSPQTVKRCVARV
                     QPTDLRAAARAFFRPERLSLAIVSPLRRDPGFGRILGRVSS"
BASE COUNT         1131 a         1820 c         1854 g         1082 t
ORIGIN      
        1 atcgcccatg cacccagtag agcagcgccc caaagagtgc ggaaccgcag attcccacga
       61 aaatggcgac aagcaggacc cgtcggcgat gacgccgatt gctccggccc atccccggca
      121 ggaggtaata ccggtgatcg ccctgacttt tgcgcaatct cgacatgatc gactcctggc
      181 ccgcgtttct caccgagtcc tcactgccgc gaagccgaag actccgaaac gcgaaccaaa
      241 gcgggctggc ccggctgttt cacaaagctt cacggagtct gacatccggg ctctggcaaa
      301 accgtgccca aaaagggaac cgggagcatt ctgtcggtcc tgagaatggt ctctcatgcg
      361 aacagtgtga tgcagtgctc gatcgctcga acctggcgcg cttttgcgtg gctagcgcgc
      421 atgagtgcat ctttcgatga catcggctcc gcgttcttca gaagttggtg agaaatgcgc
      481 gccaggggtg tgaaatatgc gggctagcgg atgcgcaccg acgcgtgctg ctggagccaa
      541 acggcatccc ggctgttggg cacgggataa tcgtgggaat gccagctcaa tcccctgggc
      601 gggttataag tccgcgaatc gacccatttg tggatttcca cgtgcgcgtc cgcgaacgca
      661 aacgtgctcg atggtttgtg gtaattggcc gggacgtggt agaaggccgg tcgcgtcata
      721 atcatgccga agaacggacg gcagatgctg ttatgatgga tttcggaaaa gacgaaaatc
      781 tccgaaggac cgacttcggt gaagtcgccg ctgcgggtgt acaccttgta ccccgacggg
      841 ggattgtccc ggtaagcggg tcccttccat ccgaccattg agttcatgcc gtaactgcgg
      901 acgacgggca ctttcttgtt gccgacccgt tcgagggttt tgtcactggg gcagcgatag
      961 atgtcggtcg agcgcagata gggtccaaag agcgagtact tcggatcgat cagtttgaaa
     1021 atattggtta tgtcggactc ggcggtcgcg aagctgccgc cgacccaggt gagcggaacc
     1081 gtggcctcgg tgccactgcc attgagaacc agggcttcgt tgttgtcgcc ggcatacatc
     1141 gtccagatca cgcccaactg tttcagattg ttcgcacatg aaatcgcggt ggcttttgcc
     1201 ttggcgcggg aaagcgccgg caagagcatc gacgcgagaa ttgcgatgat tgcgatcacc
     1261 acgagcaact cgatcagggt gaagccggcg cgggtgcggt gtgaggccgc ggggcgggcc
     1321 gatgcgaagg gccaatccat gtttgtcata atcgtatgct cagtctcaac agcaggattc
     1381 ttgcctgttc gacaaccata cgacgcccgg atagcagcaa acaagtgtgc atttgccggc
     1441 acagcggggg cgttccggga cggctcgagc ctcgatcaac ccgatatcaa ccgcccgtcc
     1501 tcgagctcga tcgcgtgcgg cacgcgggcg gcgacgcggg agtcgtgggt ggcgatgatg
     1561 agggtcgcct ggtgctcggc ctgcagatcg cagagcaggt cgatgatccc gtccccggtg
     1621 tgcgtgtcca ggttccccgt cggctcgtcg gccaggatca agttgggcgc gttgaccagg
     1681 gcgcgggcga tggccacgcg ttgttgttcg cctccggaca attcgcctgg ccgatggtcc
     1741 aggcgatgct cgaggccgac gcgccggagc aactgggcgg cacggcgcga ggcctcagtc
     1801 gctggaacgc gtgcgatgcg cgccggaagg ccgacgtttt cgagggcgtc caattcaggc
     1861 aagaggtggt aagcctggaa gacgaagcca atccggcgat tccggaaaac cgccagttct
     1921 ttcgccgaca tgacggcgag gctgcggcca tccaccaaca catcgcccgc ggtgggtgtg
     1981 tccaatccgc ccagcaaatg gagcagagtg cttttgccgg ctccggaggc tccgcgcaag
     2041 gccaggaagg ccccgcgggg gacctcgagg ctcacgcccc ggaggacctc gacagcccgc
     2101 gaccccatcc ggtacgcctt gtgcaaatcg cgtccagcta ccagagcggg cccggaggcg
     2161 tgaggatcat cattactcat ggcgaagggc ctcgaccggc ttgagccggc cggcattcca
     2221 ggcggggatc aaccccgcca gcacgcagat gatcagggac ccgccgcaaa tcacggcgag
     2281 gtcctgaggc aagatcaggg ccggcaactc gttgaagtag tagatttgag cggggaagag
     2341 atcgaacccg gtgacccggc gcataaagga gaggaagtca ttgcggtagg ccaccgccag
     2401 cagccccagc ccgaggccgg ccgtcacgcc gagaacgccc accaccaggc tttggcccag
     2461 gaacagggcc atcacctggc cggcgctggc gccgatggct ttgagggtgc cgatctcgcg
     2521 cgtcttctgg accacgaacg tgatcaggcc gctgcagatg ccgaacgcgg ccacaatggt
     2581 gatgaagaag aggaggtaga acatcatctg cttctccacc acgagcgcat cgaggatggt
     2641 cgagttctct tccatccagg tgcggatcac gtatcggccc ccgagatcgg cctgcagctc
     2701 ggctttggcc aggggcgcct ggtcgggatc gcgcagggcg accatgagcc cgtgcgcttc
     2761 attgtgaaga tcgaagaagt cctgcgcgtt ggccagcgat gtcaccaggt aagcgtaatc
     2821 gaactcgtgg aatcccactt cgaatatgcc gcgcacagtg tagtcatcgg cgacgggcgc
     2881 ctcctcctcc ccctgtttgc gcgccgagtc ccatttctcg aactggcgca cggcgtagat
     2941 ggccaccgta tcgcccaggc gcagtccgag gttcgcggcc agttggcgtc cgacaagcag
     3001 gctgtatccg cgcaaatcgt tggtgcccgc ccagaggctg tccaagagcc ggctgattct
     3061 tggctcgaag cgcgggtcga ccccgcgcac cagcggcacg atgtaacgag ggctgcccac
     3121 ctcgggctgg gtcttgatca tgacctgccc ttcgacgtac ggggcgacgc ctcgcacgaa
     3181 cgggtgcgcg gccacgagct ccatcaggcg ctcgtagtcg tgcaacggca catccgaatc
     3241 ctcgattcgg agatgcgcgt tgaacccgag tatcttctcg cggagctgtt ggtcgaatcc
     3301 gctcatgacc gcgattacga tgatgaggac cgccacgccc agcaggaccc cgacgatgga
     3361 aatcagggtg atcgccgaca caaaggtgcg tttggggcgc aggtatcgga gggccagcat
     3421 gaactcgaat ggcagccgag acatcggatc gagcctacgg acggcgttcg cccgcgtcaa
     3481 tttggctttg agcaccgcgc tgcccggcgc ttgtcacacc ggggccgaat ccctatggtt
     3541 cgtcggatga ccgatcagcc ctatggtttt gcgcgggaag acccctggcg catcttccgc
     3601 atcatggccg aattcgtgga ctccttcgag accctggcca agctgaggcc ggcggtgacg
     3661 atcttcgggt ccgcgcgcac gtcgcccggc gacaagtact acaagctggc ggagacgctg
     3721 gccagggagt tgggcaaaca tcgtctggcg ctgatcaccg gcggcggccc cggcatcatg
     3781 gaggcggcca ataaaggcgc cgctgaaagc aaggcgcact ccgtcggcct caacatcgaa
     3841 ctccccttcg agcagaaggg gaatcgtttc gccaacgtcc cgctccattt ccactacttc
     3901 ttctcgcgca aggtctgctt cgtgaagtac agcattgctt tcgtgttcat gccgggcggc
     3961 ttcggcacgc tcgatgagtt cttcgaggtc atcaccctgg tgcagaccca gcggattccc
     4021 cgcttcccct tgatcctgtt cggtcggcac tactggaacg gcctgctgcg ctggtgcaaa
     4081 cggacggtcg aggcgcctgg attcatcagc cccggcgacc tcgatctcgt cactctcacc
     4141 gaggatcctc atgaagcggt cgagattatt ctcgattacc tgcgccgggt cgggccgccg
     4201 gaaagcatcc ccaaggcctt ttgctgatgc accgcccagc ccgcatgttt catgcagttg
     4261 tgatcgccgc aaactggata actccaagat gcggaccgca tcgggccagc ccggatcagg
     4321 ttccgcgcga agcctctgat cgatccgggc cagtctcctt gaccgatgaa gcaggttgtg
     4381 gacaagtcaa gcgttagtgc cgcgttcaag gggtcaagga ttccaagcaa cgaggcgcgg
     4441 gagaaaagag cggggagaca cgggcactgc tcatgaaaag gcggactgga tttgatctga
     4501 ggcgcgcctc gtttgtcctt ctggacaatg gaatccgagt ggccctggcg cctatgccgc
     4561 acatggcgag cgtcagcctg ggtttctgga tcggggttgg cgggcggcac gagccgccca
     4621 gcctcaatgg catctcgcat ttcatcgagc acatgctctt caagggcaca cgccgccgct
     4681 ccgcccgcca gatttccgag gcggtcgagg gcatcggcgg gtatctcaac gccttcacca
     4741 gcgaggaaca cacctgtttc tactcgaagg cgcggcacga ccgtttcccg gaactgctcg
     4801 atgtcctgac ggatatgctc cttcattcgc gattcgcccc cgatgacatc gagaccgagc
     4861 gcgatgtgat ccgggacgag atcgcgatgt atctcgacca accgcaacag cacgtccagg
     4921 aactcttgaa tgccgtctcc tggcccaggc atcccctcgg acgccccttg acggggacca
     4981 agcgcacagt cagcaccctg acacgcaggc aactccaagc ctaccaccgc cagcattatg
     5041 tcggggccgc gaccgtcatt gcggtcgccg ggcacttcaa tctacagcca acccttagga
     5101 cgattcgacg gctgacgcgt ggctggccaa ccggccagcc ggatgtcttc ccgccggtca
     5161 ccgcccgcaa tcgcggaccg gcggtgcgac tggccacccg gcggatcgaa cagacgcatt
     5221 tggcgttagg aatccggact tgctctcgac acgatgagcg ccggtttgcc ctgcgcctgt
     5281 tgaatgtcat cctgggtgag aacatgagtt cgcgcctctt tcagcgaatc cgtgaggatg
     5341 ccggcctggc ttactcgatt tacagcagcg tcagtttctt cgcggacacc ggggatctgg
     5401 tcatctcaac cgggctcgac accgaacatc tcgaccgcgt gctgcgcctc atcgtggcgg
     5461 aactgaggcg tctggtggtg gagatgccgg gccgcgccga attccacagg gcccgggact
     5521 acgccctggg ccagatcgac ctcgggctcg aaaacacgga gaaccacatg ttgagcgtcg
     5581 gggagcaagt cctcggttac ggcaggccgg tctcgccgca gactgtcaag cggtgcgttg
     5641 cgagggtgca acccaccgat cttcgcgcgg cagcccgcgc cttcttccgc ccggaacgcc
     5701 tcagcctcgc aatcgtcagc ccccttcgac gcgatcccgg gtttgggcga atcctcgggc
     5761 gtgtgtcgtc ctgacatgat gaaagccagc ggatgggcat cgggactcca atgaaccgcc
     5821 cgtttcgtag ccgcggacgt aagtccgcgc ctggagtttg gacattctga agacggatta
     5881 tggcggg
//
LOCUS       DAOQRO010000228         5882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13734_length_5882_cov_4.868886, whole genome shotgun sequence.
ACCESSION   DAOQRO010000228 DAOQRO010000000
VERSION     DAOQRO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13734_length_5882_cov_4.868886"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1034
                     /locus_tag="PKK20_09110"
     CDS             246..1034
                     /locus_tag="PKK20_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNV00084.1"
                     /translation="MERFKIEEGAKTLVLGWSEENRLFAGFDVTKHMISMSGRSPSLQ
                     IRRETLAAAAASGFSVQPRGNEEIAIAFRPDFFCTYVHELHNLHRTAGQPEDLAVLRQ
                     LAEEPTAEAIRDIPPSPRRTVLQQIQRKVRDQRFRRSILVAYDYRCAMSGIQLDLLEA
                     AHIIPVEHERGTDEIKNGICLSAIHHRAFDQGLLGIRPDYSVTMNQRRLGQLRSLGWD
                     GGIELFKTTLRDQIILPARRELYPDPDYLTFGQVLRGWPERSVA"
     gene            complement(1972..5745)
                     /locus_tag="PKK20_09115"
     CDS             complement(1972..5745)
                     /locus_tag="PKK20_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNV00085.1"
                     /translation="MTRPPSINLAGLMGALLCVVCHSVEVPPLPPWQDVAGYRVRPVV
                     QPPGKRPGFTLLSPAQSGVTFTNVLTPTRAAENQIRLNGSGVALGDADGDGWCDVYLC
                     GLENENALYRNLGNGCFTNITALAGVACGGQYSSGAVFADVDGDGDLDLLVNGVGTGT
                     RLFLNGGRAVFAEQPEAGLARTYGATTAALADADGDGGLDLYVANYRTTTVRSTGFVV
                     LNVGGRRMIRPQDRDRLELTPEGRVLEHGEPHFFYRNDGGGRFSIASWTDGSFLDEDG
                     RPLERAPRDWGLSAMFRDLNGDGAPDLYVCNDFHSNDKIWINDGRGRFRLIDRLAIRN
                     SATFSMAADFADVNRDGHDDILVSDMMSRQHGRRLMQDAGMDSYSVRVGVFDDRQQFD
                     RTVLQLNRGDGTYAEMAYYAGVPASEWTWSVVFLDVDLDGFEDILCTTGHMFDTQDLD
                     AQAMIAARGPWPRERVPEKLLLLPPLHQANLIFRNLGSLRFAEAGRDWGFDQVGVSQG
                     IALADLDNDDDLDAVVNNLNGAAGLYRNDTTAPRVSVRLRGLPPNTRGVGAKLRLHGG
                     AVPLQSQEVICGGRYLSGDDGSRTFAAGQPTNRMRLEVIWRSGRRSVIDGVRADSCYE
                     VFEPADAPVAARLGANPERPLFEDVSSRLGHRHVETPFDDFARQKLLPNRLSQLGPGV
                     AWHDLDGDGWEDLIVGAGKGGKLAMFRNNGQGGFSRQEVPPGDPTASRDQTGVVGWRA
                     GEAARLVVGHAHYEDGESAAGGSAVTVLDMAGRGPAEGLPADESSVGPLALADYDGDG
                     ELDLFVGGRVIPGRYPLAASSRLYQGKGGRFTLDAAQTALLHHVGLVSGAVWSDLDGD
                     GWPDLALACEWGPVRVLLNDRGRLRVWDVAVNVQRSTFNVQHRTGSEAFPNNPHPTTL
                     NEMTGWWNGITAGDLDGDGRLDLIVSNWGLNTKYRASPDSPRRLYYGDFTGSGGVDLI
                     EAFFDPGRRQDVPERDRDAVAAMMPALNARLPTHLDYGRATVAEILGEAAGRAARVEA
                     TELASMVFYNRGDQFEAKALPFEAQLAPAFGVAVDDCDGDGLDDVFLAQNFFAMQSKT
                     SRADAGCGLWLRGDAQAGLVAVPGQESGIKVYGEQRGCALTDCDADGRVDLVVAQNAG
                     ETKLYRNVRARPGLRVRLDGPLGNPTGVGAVVRLGTGGRWGPAREMHAGSGYWSQDGA
                     VQVMASPRTFGTMPAQIKVRWPGGRETVSTIPPDVRQIEVGLDGRLRVRAVGR"
BASE COUNT         1136 a         1976 c         1762 g         1008 t
ORIGIN      
        1 cgtgctgaat aacgacactc gtcagaacta tgccgagccg tatccccaaa cgcgcgatac
       61 tcacccgcgt tcttgaagcc attcgtgcct ctgggtggaa ctgcataatc ctgtccgact
      121 cccacccctt ccgcttggct gtctcccgag gcgagacacg tgagagagtg ctctgttaca
      181 gttggaatct gactcatggc ggctacccaa gggatccgaa cgagctgcga atccaagtga
      241 ccggcgtgga gaggtttaag atcgaggagg gcgcgaagac attggtgctc ggatggagtg
      301 aagagaaccg cttgttcgcc ggcttcgatg tcaccaagca catgatatcg atgtcgggcc
      361 ggtcaccgtc tctgcagatt cgtcgcgaaa ccctggccgc tgccgctgca agtggattca
      421 gtgtgcaacc gcgaggcaat gaggaaatcg caatcgcctt tcgtcctgat ttcttctgca
      481 cgtacgttca cgaactgcac aacctgcacc ggacagcggg ccaaccggag gacttggcgg
      541 tcctgcgaca actcgccgag gaacctacgg cggaggcgat tcgcgacatt ccaccgagtc
      601 cccggcgaac ggttctccag cagattcagc gcaaagttcg cgatcagcgg ttccgaagaa
      661 gcattctggt cgcctatgat taccgatgcg cgatgagcgg gattcagttg gacctactcg
      721 aagctgcgca catcatcccg gtcgagcacg agcgcggcac cgacgagatc aagaacggca
      781 tttgcctaag cgcgatccac caccgggctt ttgaccaggg acttctgggc atccggccgg
      841 actactctgt gacaatgaac cagcgacgcc tcggacaact caggtcactc ggctgggacg
      901 ggggcatcga attgttcaag actacgctgc gcgaccagat catcctgccc gcacggcgag
      961 aactctaccc cgatcccgat tatctgacct ttggccaagt ccttcgcggt tggccagaac
     1021 ggtccgtcgc ctaacccgca tacttggcag cgaggcaaag ccgcaaccaa actgtgcaat
     1081 gcctggcggc ctttgcggca tgatacttct ttcaaagaga aaagaagatg aagcgtttga
     1141 tccacccgac cgacgtacgt ctccccggtg cgcaatgagt cgtgctcgag ccgaagtgcc
     1201 tcgcggctgc aaatcgctct tgccaagccg cttggcaagc atagcctccc aacgtgatgc
     1261 aaaagattgc gtcacttcgg atgtccatta cttgtcgccc gagccagcct cggcaccgcc
     1321 gatcagcaga acgagttgcc accagaccgc cggacggaca accacgagca gtcggcagtg
     1381 ttccccttat ccgtgtgatc cgtgtgatcc gtggctcatt cttggcttgt tcttggcttg
     1441 ttcttgggcc accgattaca cggatttcac ggatgggcgg ggcgccgggg acgggacgtt
     1501 gggccggaaa gggcgaatca gacggtggag gcaacggcga cccagccact ggctttgatg
     1561 gctggacccg gtgacgtccg tggtgtctgg ggtcgccgtg cctcacctgg tcgttcggcg
     1621 gcaagtggtc acggaccgat gatcagaatc ccctcgcacc agaccgccgt gccgacggcc
     1681 acaaaccgtt tgcgggttgc tttggattcg tgtcattcgt gtcattcgtg gtccagttcc
     1741 tggggggaaa ggagaaccac gaatcacacg aatggcagag acgaactctg gcggctccgt
     1801 ggtccctggg caggactgtc gaagcagcga ggagcccgat tgctgcaccg agaccgatgc
     1861 cgcttgggac gcggagattg gctgccggat tgccggcttc gacgtcacca cacacatgat
     1921 attgatgtcg agccgatcgc catctccgca gcttcgcctt tctgccggac ttcagcgtcc
     1981 aacagcccgc actcggagac ggccatccag tccaacctcg atttgccgca catcgggggg
     2041 aatggtggac accgtctcgc gcccgcccgg ccaccgcacc ttgatctggg ccggcatggt
     2101 cccgaacgtt cgaggtgaag ccatcacctg caccgcaccg tcctgcgacc agtagcccga
     2161 cccggcatgc atctcccgcg ccggccccca acgaccgccc gttcccagtc ggacgaccgc
     2221 cccgaccccg gtgggattgc ccaaggggcc gtcgaggcgg acgcgcaggc cggggcgggc
     2281 gcggacgttt cgatacagct tcgtttcgcc tgcattctgc gcgacgacaa gatcgacccg
     2341 gccgtccgcg tcgcagtccg tcaaagcgca gccgcgttgt tcgccgtaga ccttgatccc
     2401 cgattcctgg cccggcacgg cgacaagacc cgcctgggca tcgccccgaa gccaaagtcc
     2461 gcacccggca tcggcgcggc ttgtcttgga ctgcatggca aagaaattct gcgccaggaa
     2521 aacatcatcg aggccatcgc cgtcgcagtc gtccaccgcg acgccaaagg cgggggccaa
     2581 ctgggcctcg aacgggaggg ccttggcctc gaactgatcg cctcgattgt agaataccat
     2641 cgaggccaac tcggtcgctt ccactcgagc cgcgcgtccg gcggcctcgc cgagaatctc
     2701 ggccacggtg gcgcggccat agtcgagatg cgtcggcaac cgagcattca acgccggcat
     2761 catggcagcg acagcatcgc ggtcccgctc gggaacatcc tgccgccgtc cgggatcaaa
     2821 gaacgcctcg atgagatcga ccccgccgct cccggtgaag tcgccgtagt agagccggcg
     2881 cgggctgtca ggactcgccc ggtacttcgt gttcagaccc cagttcgaca cgatcaggtc
     2941 cagacgcccg tcaccgtcca ggtcgccggc agtgatcccg ttccaccagc ctgtcatttc
     3001 gttgagcgtt gttggatgcg ggttgttcgg gaacgcctca ctgccggttc tatgttgaac
     3061 gttgaacgtt gaacgttgga cgttcaccgc cacatcccac acgcggagcc gtccgcgatc
     3121 gttgaggagg acgcgcaccg gcccccactc gcacgccagg gccagatcgg gccagccgtc
     3181 cccatccaga tcgctccaaa ccgccccgct caccaacccc acgtgatgca ggagggccgt
     3241 ctgcgccgcg tccaacgtga accgtccgcc cttgccctgg taaagacggg acgacgccgc
     3301 gagaggatag cgtccgggaa tcacgcggcc acccacgaaa agatcgagtt cgccgtcgcc
     3361 atcgtagtcg gcgagggcaa ggggaccgac actcgattcg tccgcgggaa ggccttccgc
     3421 gggaccgcgg ccggccatgt ccaacaccgt gaccgcactg ccgccggcgg cgctctctcc
     3481 gtcctcgtaa tgggcgtggc ccacaaccag acgcgcggcc tcgcccgcgc gccaaccgac
     3541 cacgcccgtc tgatccctcg acgcggttgg gtcgcccggc gggacttcct gacgactgaa
     3601 tccgccctgt ccgttgttgc ggaacatcgc cagcttgccg cccttgcctg cgcccacgat
     3661 caaatcctcc cacccgtctc catcgagatc gtgccaggcc acgccaggcc cgagctgact
     3721 gagccgattc ggcaacaatt tctggcgcgc aaaatcgtcg aagggagtct cgacatgccg
     3781 atggccgagg cgactcgaca catcctcgaa taaaggccgt tccggatttg cccccagccg
     3841 ggccgccacg ggggcgtccg ccggctcgaa cacctcgtag cacgagtccg cgcgcacgcc
     3901 gtcgatgaca ctccgacgcc cgctgcgcca gatcacttcg agccgcatcc gattggtagg
     3961 ctggcccgcg gcgaatgtcc gcgatccgtc gtcgcccgaa agataccgtc cgccgcaaat
     4021 cacttcctgg gattgaagcg gaacggcgcc gccgtgaagc cgcagtttcg cgcccacccc
     4081 ccgcgtgttg ggcggcaggc cgcgcaagcg cacactgaca cgcggcgcgg tcgtgtcgtt
     4141 ccggtacagc ccggcggcgc cgttgaggtt gttcaccacg gcatcgagat cgtcgtcgtt
     4201 gtccaggtcc gccagcgcaa tcccctgcga cacgcccacc tgatcgaagc cccaatcccg
     4261 tccggcttcg gcaaatcgga gagatccgag gttgcggaag atgaggttgg cctgatgcaa
     4321 cggcggcagc agcaggagct tctcggggac gcgctcccga ggccaggggc ccctggcggc
     4381 aatcatcgcc tgggcatcga gatcctgtgt gtcgaacatg tgccccgtcg tgcaaaggat
     4441 gtcctcgaac ccgtccaaat ccacatcgag aaagacaacc gaccacgtcc attccgatgc
     4501 cggcacaccc gcgtagtagg ccatctccgc gtaggtgccg tcgccgcgat tgagctgcag
     4561 cacggtgcgg tcgaattgct gccggtcgtc gaacacaccg acccgcacgg aataggaatc
     4621 catgccggca tcctgcatca ggcgccgccc atgctgccgg ctcatcatgt cggacaccag
     4681 gatgtcatcg tgaccgtccc ggttcacatc ggcgaagtcg gctgccatcg agaaggtggc
     4741 ggagttgcgg atggccagcc ggtcgatgag ccggaaacgg ccgcggccat cgttgatcca
     4801 aatcttgtcg ttcgagtgga aatcgttgca gacatacaag tcgggcgcgc cgtccccgtt
     4861 caggtcccgg aacatggccg acaagcccca atcgcgcggc gccctctcga gaggccggcc
     4921 atcttcgtcg agaaaagacc cgtcggtcca cgatgcgatg ctgaagcgcc cgccgccgtc
     4981 gttccggtaa aagaaatggg gctccccatg ctcgagcaca cggccctccg gggtgagttc
     5041 gagccgatcg cgatcctgcg ggcggatcat ccggcgaccg cccacgttga gaacaacaaa
     5101 acccgtcgac cgcacggtcg tggtccggta gttggccacg tacaggtcaa ggcccccgtc
     5161 gccatccgca tcggccagag ccgcggtcgt ggcgccatac gtgcgcgcca aaccggcctc
     5221 cggctgttcg gcgaagaccg cccggccccc gttgagaaac aagcgcgtgc ctgtgccgac
     5281 accgttgacg agcaaatcca ggtcgccgtc gccatccacg tcggcaaaca ctgccccgct
     5341 cgaatactgc cccccgcacg caacgcccgc caatgccgtg atattggtga aacaaccgtt
     5401 gccaaggttg cgatagaggg cgttctcgtt ctcgagcccg cacaaataga cgtcgcacca
     5461 tccgtcgcca tccgcatcgc ccaacgcaac acccgacccg ttgagccgga tttggttttc
     5521 cgcggcccgc gtgggcgtca acacgtttgt gaaggtcaca cccgactgcg ccggggagag
     5581 cagcgtgaag ccgggacgct tcccaggagg ctggaccacc ggacgaacgc gatagccggc
     5641 cacgtcttgc caggggggca agggaggaac ctcgaccgag tggcacacga cacaaagcag
     5701 cgcgcccata agccctgcca ggttgatcga aggcggtctg gtcatcgtgg gctgcccagt
     5761 gcaacgttca aaatccaacg tccaacgaac tgctcaggtg aacgttgaac gttgaacgta
     5821 ccaacggttt gcgcacacag tgcatgggga gcgttgacat tcatctgctg aatacgcgtg
     5881 ga
//
LOCUS       DAOQRO010000229         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13808_length_5860_cov_3.651680, whole genome shotgun sequence.
ACCESSION   DAOQRO010000229 DAOQRO010000000
VERSION     DAOQRO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13808_length_5860_cov_3.651680"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PKK20_09120"
     CDS             complement(<1..1227)
                     /locus_tag="PKK20_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV00086.1"
                     /translation="MIDLNFSSSASSVCLWAVVGLIAARCAAEWSLERLNQRHVRAHA
                     GRVPDAFAGVVDQPTYVRSVQYTLAKSRFHELECLWGTVVLVAILLSGVLPWTYTQFA
                     EACGVTPWALALFLLAVGVCLSFTDLPFDWHRQFRLEARFGFNTTTPRLWWMDRLKGL
                     LLSLVLGYPLLVLVLKIVEWTGPWWWLWAWAAVLGFQFLILLLAPVLILPLFNKFTPL
                     AEGTLRERLLALAARIRFRAQSIQVMDGSKRSRHSNAFFTGFGRFRKIVLFDTLIQQL
                     AEPELEAVLAHEIGHYRKRHVLKLLAWSALTLLAGFYAIAWLADQPWFFHAFGFIPGN
                     IMPALLLFVLLSDAVMFWFSPLANLWSRRFEYQADAFAAEVMGDSQSLIGALRKLNEK
                     NLSNLTGSYSKSNTAKR"
     gene            1662..5192
                     /locus_tag="PKK20_09125"
     CDS             1662..5192
                     /locus_tag="PKK20_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNV00087.1"
                     /translation="MGLTFRTACVAILGVGAVLMASEPQDIAFVATHDGSEQRYVLIL
                     PEPFDTAQSHDLLICLHGHGSDRWQFAREDRGEARAARDTAARHRMILVLPDYRAPTS
                     WMGPAAEADVVQIIHDMKGRHRIGGVVLAGGSMGATGALTFTALHPELVDGVVALNGH
                     ANHIEYSGFQEAIRSSFGGTKEAVPDEYRKRSAEFFADRFTMPVAITAGGRDATVPPD
                     SVLRLAGSILKHNPSVYLDYVQTREHETDYEASRAAFAFVVRALGTTPIRIGVSINGK
                     SLLPAHGSAAGIWFYADGADGSQLLLTGHAEVPGSWRLTASLNAGDRVRIAFGGDEPP
                     PIAVGFQAHALSSEPIECRAESGALVLEAGAAAGSVTLTHFAVRAMPVSFAPERRPFS
                     RAPVASSPDPRPAIAEALIEWDWQMQDGIQTPREARSFREAVGKLVRRTDALVAERLR
                     LGTLAPEQQLAWRELRAISEPAEGDASGWEARWLDVHRLRRRIVLSNPVFQFPALLFV
                     KHVPSVMSHQLTQMYGYCARPGGGLFVLENPGLGMRTRNITPPAMAPGNFMTPELSHD
                     AERLLFAYCPVKEAPMSWDFNDQTRHLRYHIHELSLSSGAVRALTRGDTDNFSPVVLP
                     SGEILFLSTRRGGYHRCGRGPCFVYTLARMGAGGEDPRSVSFHETHEWDPSLLNDGRV
                     VYTRWDYVDRNAVHYQQLWSAFPDGGDARIYYGNNTWNPTGIWEARAIPGSSRIMATA
                     APHHGMSAGSVILVDTTRGVDGPAPLTRLTPEVRFPESESPLAFGPSPATYDFDTPST
                     HFWSSPLVERWAEQTVPEEEKRWPGHCYKSPWPLSEAFFIVSYSYDRLVGEPGPNIPN
                     MFGLYCADAFGNRELLYRDPAISSLWARPLVRRAPPPNLAMPISNPESGTGTFFLQNV
                     ARSWPRLLPDTRIAQLRIIQVLIKTTPNADSPRVGAAFAAPGKQVLGTVPVEPDGSAF
                     FEVPARTPVMFQALDARGRAVQTMRSLVYLQPGENQSCIGCHEHRMTAVVGGAAPQAI
                     RRAPSKIRPGPDGSRPFSYPRLVQPVLDRLCVQCHDGREPGRPVLTGEPEAPFSRSYN
                     ALVSRVSFSAWNRPEQNFEPLTEPMRFGALGSALAKMLEEGHGEVVLTPEDEERLYTW
                     MDANALFYGTFDVVEQGKQLAGEAIEGPKQ"
     gene            5407..>5860
                     /locus_tag="PKK20_09130"
     CDS             5407..>5860
                     /locus_tag="PKK20_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00088.1"
                     /translation="MTTNPQLKAWFQGVQDHKRYELKKVLGVEYAHLILDTGDLYVTA
                     DGLVFLKLLDPSNYWFDKEWFDSHAVQLSGTSSIHKIRTKTVDGRSRDIVMKWNRMGQ
                     DIPGAEEVDALEGAAFNSPFEEFSLVMELRGKLYGMGRRVVVQKPLAIF"
BASE COUNT         1044 a         1743 c         1986 g         1087 t
ORIGIN      
        1 gcgtttggca gtattgcttt tggaatagga accagtcagg ttgctcaggt ttttctcgtt
       61 gagttttcgg agcgcgccga tcagggactg ggaatcgccc atgacctcgg cggcgaaggc
      121 gtcggcttgg tactcgaaac ggcgggacca tagattggcc aggggcgaga accagaacat
      181 gaccgcgtcg ctcagcagga cgaacagcag gagtgcgggc atgatgttgc ctgggataaa
      241 gccgaaggca tggaagaacc agggctgatc ggccagccat gcgatggcgt aaaacccggc
      301 caacagggtg agggctgacc aggccagcag cttgaggaca tggcgtttgc ggtagtgtcc
      361 gatctcatgg gccagcacgg cttcgagttc cggctccgcc aactgctgga tgagcgtgtc
      421 gaacaggacg atcttgcgga agcgtccgaa tcctgtgaag aacgcgttcg agtggcgcga
      481 tcgcttgctg ccgtccatta cctggatgct ctgggcgcgg aagcggatgc gtgcggccag
      541 ggcaagcaat cgctcgcgca gcgttccctc ggccaagggg gtgaacttgt tgaacagggg
      601 gaggatgagc actggcgcga gcaggaggat gaggaactgg aagccgagca ccgcggccca
      661 cgcccagagc caccaccacg gcccggtcca ttcgacgatc ttcagcacca gaacgagcag
      721 gggatacccg aggaccaggg aaagcagcag acctttgagc cggtccatcc accagaggcg
      781 aggggtggtt gtgttgaagc cgaagcgcgc ctcgagtcgg aactggcggt gccagtcgaa
      841 gggaagatcg gtgaacgaga gacagactcc gaccgccaac aggaacaagg ccaaggccca
      901 gggcgtaacg ccgcatgcct cagcaaattg cgtgtaggtc cacggcagaa ccccgctgag
      961 cagaatcgcc acaagcacca cggtccccca gaggcattcc agttcgtgga aacggctctt
     1021 cgccagagtg tattggaccg agcgaacgta ggtgggttgg tcaaccacgc cggcaaaggc
     1081 gtcgggcacc cgtcccgcat gcgcgcggac gtggcgttgg ttgagccgtt cgagtgacca
     1141 ctccgcggcg caccgggccg cgatgaggcc gaccacggcc cagagacaaa cgctcgaagc
     1201 gctcgagctg aaattcaggt caatcatccc gtcgactcta cgatgtaccg ccggcagagg
     1261 cgatcacttt gggtggagtg gtggggggcg atcaaaacca ctttgggtgg gttgacaccg
     1321 ccctgggacg gtgcgggcaa tggtgcgcga ggttgtcagg gaggtagcaa gcatgaagtc
     1381 agcgatcaaa gggaccatag ggacgcaagg gcattgtgaa caattccgcg cccgcggtcc
     1441 tgtggtcgct ctcgtctctt gggtcccttg catgttatga aatccatgcc ctaaggcttc
     1501 atccccgcgc acggcggcca ccgtaaactg ccgcagactc caggcgctct tgaagatgca
     1561 cttgaaggtt gaactggtgt ttcggccgcc taccctgcgc cggtgacttc gaacgagaac
     1621 cgccgactgc ttcgagggca gggcgccgga gcccgatgaa catgggcctg acgttccgga
     1681 cggcgtgcgt ggcgattctg ggcgttggtg ctgtcctgat ggcgagcgag ccccaggaca
     1741 ttgcctttgt cgccactcat gacggcagcg agcagcgtta tgtgctcatt ctgcccgagc
     1801 cgttcgacac cgcccagtcg cacgacctgt tgatttgcct gcacgggcac ggctcggatc
     1861 gctggcaatt cgcccgggaa gatcgcggag aggcccgtgc cgcccgggac acggctgcgc
     1921 ggcatcggat gattctcgtt ctgcccgatt accgggctcc gacgtcgtgg atggggccgg
     1981 ccgccgaggc ggatgttgtc cagatcatcc atgacatgaa gggccggcac cgcatcgggg
     2041 gcgtggttct tgcgggaggg tccatggggg cgacgggcgc cttgaccttc accgcgctgc
     2101 atcccgaatt ggtggatggc gtggtggctc tcaacggcca cgccaatcac atcgagtatt
     2161 cgggctttca ggaggcgatc cggagctcgt tcggcggaac caaggaagcc gttcccgacg
     2221 agtatcgcaa gcgaagcgcc gagttctttg cggacaggtt caccatgccc gttgcgatca
     2281 ccgccggggg gagggacgcc acggtgccgc ccgacagcgt tttgcggctg gccggctcga
     2341 tcctgaagca caatccgtcg gtctatctcg actacgtcca aacacgggag catgaaaccg
     2401 attacgaggc atcgcgcgcc gcttttgcat tcgtcgtgcg cgcgctcggc acgactccga
     2461 tccgcattgg cgtttcgatc aatgggaaga gcctcctgcc cgcgcacggc tcggcggcgg
     2521 gaatctggtt ctatgcggac ggcgcggatg ggtcgcagtt gttgctcacg ggccacgccg
     2581 aagtcccggg atcgtggcgc ctgacggcat cgctgaacgc cggcgacagg gtgcggatag
     2641 catttggcgg agacgagccg ccgccgatcg ccgttggctt ccaggcgcac gcgctgtcgt
     2701 ccgagccgat cgagtgccgc gcggaatcag gcgcgcttgt tctcgaggcc ggggccgcgg
     2761 ccggctcggt gacgctgacg cattttgccg ttcgggccat gccggtttcg tttgcgcccg
     2821 agcgccgtcc gttttcgcgc gcgccggtgg cgtcgagtcc ggatccgcgt ccggcgattg
     2881 cggaggcgtt gatcgagtgg gactggcaga tgcaggacgg cattcaaacg ccccgcgaag
     2941 cgcggtcgtt ccgggaagcg gtcgggaagc tcgtgcgtcg gaccgatgcg ttggtggcgg
     3001 agcggctcag gctgggaacg cttgctccgg aacagcagtt ggcctggagg gagttgcggg
     3061 cgatcagcga gcctgccgag ggcgacgcgt cgggctggga ggcgcgctgg ctcgatgtgc
     3121 accggttgcg ccgccggatc gtgctctcga atccggtctt ccagttccct gcgctgctgt
     3181 tcgtgaaaca tgtcccgtcc gtgatgagcc atcagctcac ccagatgtac gggtactgcg
     3241 cgcgcccggg cggcggtttg ttcgtgctcg agaatccggg tctgggcatg cgcacccgca
     3301 acatcacccc accggccatg gcacccggca atttcatgac tccggaactc tcccacgatg
     3361 cagaacgcct gttgttcgct tactgcccgg tcaaggaagc tccgatgtcg tgggatttca
     3421 acgatcagac ccgacacctg cgctatcata ttcacgagtt gtcgctgagc tcgggcgccg
     3481 tccgcgccct gactcggggc gacacggaca acttctcgcc ggttgtgctg ccctcgggcg
     3541 agattctgtt cctgtcgacc cgacgcggcg gctatcaccg atgcgggcgc ggcccgtgtt
     3601 ttgtctacac gctggcgcgg atgggcgcgg gtggcgagga cccgcgcagc gtctcgttcc
     3661 atgagaccca cgaatgggat ccctcgctct tgaacgacgg ccgggtggtt tacacgcgct
     3721 gggactatgt ggatcgcaac gcggtgcatt accagcagtt gtggtcggcg tttcccgacg
     3781 gcggcgacgc gcggatttac tacggcaaca acacgtggaa tcccaccggc atttgggagg
     3841 cgcgggcgat tccgggcagc tcccgcatca tggccacggc cgccccgcat cacggcatga
     3901 gcgccggatc ggtcatcctg gtggacacga cgcgaggtgt ggacgggccg gcgccgctca
     3961 cgaggctgac gcccgaggtt cgctttcccg aatccgagtc gcccctggcc tttggcccga
     4021 gtccggcgac gtatgatttt gacacgccgt caacgcattt ctggagcagc ccgctggtgg
     4081 aacgatgggc ggagcagacc gtgccggagg aggagaaacg ctggccgggg cattgctaca
     4141 agtcgccttg gccgctttcc gaggctttct tcattgtctc gtacagctac gaccggcttg
     4201 tgggcgagcc ggggccgaac attccaaaca tgttcggact ctattgcgcg gacgcctttg
     4261 gcaataggga actgctctat cgcgatcctg ccatttccag cctgtgggcg cgtccgcttg
     4321 tccgccgggc gcctccgccc aatctcgcca tgccgatctc caatccggaa agcggcacgg
     4381 gcaccttctt cttgcagaac gtcgcgcgga gttggccgcg tctgctgccg gacactcgca
     4441 tcgcgcagtt gcggatcatt caggtcctga tcaagaccac ccccaatgcc gattcgcccc
     4501 gcgtgggcgc cgcgtttgcg gcacccggca agcaggtgct cgggacggtg ccggtcgaac
     4561 ccgacggctc ggctttcttc gaggtccctg cccggacgcc tgtgatgttt caggcactcg
     4621 atgcccgtgg acgggcggtg cagacgatgc gcagtctggt gtacctccag cctggcgaaa
     4681 accagagctg catcggatgc cacgaacacc ggatgacggc ggtggtcggc ggcgcggccc
     4741 ctcaggcgat ccggcgcgcc ccttcgaaga ttcggcccgg cccggacggg tcgcgtccct
     4801 tcagctatcc gcgtctggtg caaccggtgc tcgatcggtt gtgcgtccag tgtcatgacg
     4861 ggcgcgagcc cgggcgtcct gtgctcacgg gcgagccgga ggcgccattc agcaggtcct
     4921 acaacgccct ggtcagccgc gtctccttct ccgcctggaa ccggcccgag cagaacttcg
     4981 agccgttgac cgagccgatg cgattcggcg cgctggggag cgcgctggcg aaaatgctcg
     5041 aagaaggtca cggcgaagtg gtgttgacgc ccgaggacga ggaacggctg tacacgtgga
     5101 tggacgcgaa cgcgcttttt tacggcacat ttgacgtggt cgaacagggc aagcaacttg
     5161 caggagaggc gatcgagggt ccaaagcaat gagggtcggg cacagggctt gccgccaccg
     5221 tccaatatcc ttaaaaagca ttgggctgct gtgggttgag tgcgtgatgg aagggcggaa
     5281 gcatcttttc agccaagctc gttgtttttc cttcgtttcg ccttgaagaa gcgtcgttca
     5341 ctcgggcatg ctgccattgt gcgatggcgc aaacatgttt gacgggttgt gactgaggcg
     5401 cgctcgatga cgaccaaccc tcagctcaaa gcttggtttc aaggcgtcca ggaccataaa
     5461 cgatacgaac tcaagaaggt cctgggtgtc gagtatgcgc atttgattct ggacacgggc
     5521 gatctctacg tgacggcgga cggtttggtc tttctgaagc tgctggaccc ttcgaattac
     5581 tggttcgata aggagtggtt tgactcgcat gccgtccagt tgtcgggtac cagctcgatc
     5641 cacaagattc gcaccaagac cgtagacgga cggtccaggg acatcgtgat gaagtggaat
     5701 cggatgggcc aggacatacc cggagcggag gaggtggacg cgctcgaggg ggcggcattc
     5761 aacagtccgt tcgaggagtt ctccctggtg atggaactgc gggggaagct gtacgggatg
     5821 gggcgtcggg tcgtggtgca gaagccgctg gcgatcttcg
//
LOCUS       DAOQRO010000230         5846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13858_length_5846_cov_3.980832, whole genome shotgun sequence.
ACCESSION   DAOQRO010000230 DAOQRO010000000
VERSION     DAOQRO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13858_length_5846_cov_3.980832"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="hisG"
                     /locus_tag="PKK20_09135"
     CDS             complement(<1..490)
                     /gene="hisG"
                     /locus_tag="PKK20_09135"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNV00089.1"
                     /translation="MKSERKRQPKKPILRLGLPKGSLQTATIEKMGKAGYNITLSSRS
                     YVPYVDDDELEIRFIRAQEVSRYVEHGHLDAGLTGYDWIQENGSSVHEVGEFLFSKAT
                     RQSARWVLAVPEASPIRSVMDLEGRRIATEVVNITKRYLRRHGVKAQVEFSWGATEVK
                     AHE"
     gene            complement(582..1208)
                     /gene="purN"
                     /locus_tag="PKK20_09140"
     CDS             complement(582..1208)
                     /gene="purN"
                     /locus_tag="PKK20_09140"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNV00090.1"
                     /translation="MTRATQFKLGVLGSGKGSNFVAIADACAAGRVPATIELVLSDLP
                     EAGILDRARERGIRAEHLAPGRYRTRLDEAAERAYVAGLQEAGVDLVVLAGFMRILKG
                     DFLGAFPGRVVNIHPSLLPAFPGLEAWKQALDHGVKVTGVTVHLVDAGVDTGPILAQA
                     PVLVRDDDTPETLHARIQEVEHRLYPATIARWLTDTFGACARGPNLEE"
     gene            1358..3562
                     /locus_tag="PKK20_09145"
     CDS             1358..3562
                     /locus_tag="PKK20_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00091.1"
                     /translation="MKTDCSFPVQRGVLALIAAGALLHCPAPQAASYAETVLADNPVV
                     YYRLEDPADTYTITNAVNPDAYQGDVIYDDFAAFPKFEQAGIDVRSAGFHVYTPDGGT
                     PQKSHIQVPYAPELNPEGPFTVELWARATSWAAGVRCIAGNVSSYSDGWWFRQEAGTT
                     PRWIYVQNGGGIYMAGGNIAKNEWAHLVVTYDGTTVRFYGNGEQQWTSTGTVPSINLG
                     GPLCLGGNPAINEEFFDGNIDEVAIYSSALTADQVRLHHEVGRANIALPPVPASIVQG
                     PASTSAYAGRIATFAVNADGTSPLAYQWHRNDSPIDGATNDILSFTCAYADNGATYKV
                     VVTNPYGSATSAPATLTVMTDLTVESQPASITRTAGSKAAFIAVSGGALPVTYQWYKA
                     ADPIPGATDQTLWLSSIQLADDQTTYSAKITNPWMSVETDPAVLTVVPRAVPVPVDGY
                     ARVVMADDPVAFWRLDEADASVTAVDAAGSFDGQYTLGSGAITYGVAPGIPQETNPAV
                     AVTGKAHVQIPWALELNPHGPFTVELWFQPASLNPGPDFRNVISSEGAGPNWNGPNGW
                     LLYHMPDDTLSWVLFSRGWDGKWLGGGGPVEAGTWYHAVLTYDGALFHTYLNGDPIAT
                     QAYPAFVPNGDGFTSLGFRFDGNGAGFEGAIDEVAFYRKALSPDQIRAHSSASVKLSI
                     ERSGDSIVLAWPFGTLQQADRIDGAFADLTAATSPWTVAIGGAAKYYRVRIE"
     gene            complement(3658..5607)
                     /locus_tag="PKK20_09150"
     CDS             complement(3658..5607)
                     /locus_tag="PKK20_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNV00092.1"
                     /translation="MLLAGLALAPGCWPVSAAAPTPLRLAGSFVQYQSELQTWAPETW
                     HAVLDRMRDLRLNTIIIQMLERENHDGTTHSFIGSSGLRDATEIILDYADAHDFEVYL
                     GLYLPNWNHDMTGGEFLAQTQGRMASTAQRAWARYQAGLPHRSFAGWYIPYEPWTADY
                     QPAEVARLKSFFRGIAAACAVISGDAPLAISPFISNQRPSPCRVEKVYRELLDQTGIG
                     LLLLQDSVGAQQWETDIVQRVAPYAQAFKNACDAAGVRFWANLESFRISDGVYMPCDA
                     LRLRRQFDATAPYVERFVTFDFLHYMNPVAFLSHWNQARREGMQRLYADYQTAFVSAD
                     YDPWASPTIAARLRGEQLTLIWEGNAGDRFQLQSMTDLLGVWTPVDAPITTNQTGAFL
                     ADTSPAPHRTRFYRVQRLSRLEMADSMRWVPAGEFVMGTPAEDTNRTAGELGQFRAGF
                     TRGFWISRHEVTQSEYQNLMCTNPAAYATNLDCPVERVSWHEAMDYCARLTQQERQAG
                     RLPDGFIYRLPSEAEWEYAARAGTSSAFCYGDDPALLGAYGWHSGNSGLRPHPVGELA
                     PNSWGLEDVHGNVFEWCWDWIESSPPEPVADLIGTITGPTRAVRGGGWSFPWVNCRCS
                     WRAGYPPVARHSFLGFRVVLAQPSL"
BASE COUNT         1076 a         1948 c         1801 g         1021 t
ORIGIN      
        1 tctcgtgcgc cttgacctcg gtggcgcccc acgaaaactc gacctgggcc ttgacgccgt
       61 gccggcgcag atagcgcttg gtgatattga cgacctcggt cgcgatgcgg cgcccttcga
      121 gatccatgac cgatcggatc ggtgaggctt ccggcacggc caggacccag cgggccgact
      181 ggcgggtcgc cttgctgaac aggaactccc ccacctcgtg cacgctcgac ccgttctcct
      241 ggatccaatc gtatcccgtc aggccggcat ccaaatgccc atgctcgacg taacggctga
      301 cctcctgtgc ccggataaac cggatctcga gttcgtcatc gtccacgtac ggaacgtagg
      361 agcggctgct cagggtgatg ttgtaccccg ccttgcccat cttctcgatg gtggccgttt
      421 gcagactgcc cttgggcaat cccaatcgca gtatcggttt cttcggctgt cgctttctct
      481 cgctcttcat gaatacacaa accacaaaag cccgtcggga ccttcttttc cccccatgcc
      541 ggggtcgccc cgcggcgtcc cggttccggg cgcgcggccc gctattcctc caagttgggc
      601 ccgcgggcgc aagcaccgaa tgtatcggtg agccacctgg cgatggtggc ggggtagagc
      661 cgatgctcca cttcctgaat gcgtgcgtgg agcgtctccg gcgtgtcgtc gtcccgcacc
      721 aggaccggcg cctgggccag aatcgggccg gtgtcgacac ccgcgtctac aaggtggacc
      781 gtgactcctg tcaccttcac cccgtgatcg agcgcctgct tccaggcttc gagaccggga
      841 aaggccggca gcaaagaggg atgaatgttg acgactcggc ccggaaaagc gccgagaaaa
      901 tcgcctttga ggatgcgcat gaaaccggcc agcacaacca gatccacgcc ggcttcctgc
      961 aagcccgcca cgtaggcccg ctcggccgcc tcatccaggc gcgtccgata gcgccccggc
     1021 gcgaggtgct cggcgcgaat cccccgctcg cgcgcccgat ccaggatgcc tgcctccggc
     1081 aggtcgctga gcaccagttc gatcgtggcc gggacgcgcc ccgcagcaca ggcgtcggca
     1141 atcgccacga agttcgagcc cttgcccgaa cccaaaacgc ccaatttgaa ttgcgtcgcc
     1201 cgtgtcatgc ggacgccttg gtatcaaagc cccgcgccaa aacaagcccg aaaccgccgg
     1261 gtatttcgtt gacgtccctc tggcgcgtcc tacagtgtcc tcagaatgca tgcccatggt
     1321 cggcagagct gcaacagcaa cacaccacac ggattcaatg aaaactgact gttccttccc
     1381 cgttcagcgc ggcgtcctgg cgcttatcgc cgccggcgcg ctgctgcact gtcccgcgcc
     1441 ccaggccgcc tcgtatgccg agaccgttct ggccgacaat ccggtcgtct actatcggct
     1501 cgaagatccg gccgacacgt acaccatcac caatgctgtc aaccccgatg catatcaggg
     1561 agacgtgatc tacgatgatt tcgccgcctt cccgaagttc gagcaggctg gcatcgacgt
     1621 ccgttcggct ggatttcacg tctacactcc cgatggcgga accccccaga aatcgcacat
     1681 ccaggtgccc tatgcgccgg aactgaaccc ggaaggtcca ttcacggtgg agctttgggc
     1741 gcgggcgacc agttgggctg ccggggtgcg gtgcattgcc ggcaatgtga gcagctactc
     1801 cgatggctgg tggttccgcc aggaggccgg gacgacgcct cgctggattt acgtccagaa
     1861 tgggggcggc atttacatgg ccggcggcaa catcgccaag aacgaatggg cgcacctggt
     1921 ggtcacctac gacggcacaa cggttcgatt ctatggcaat ggcgagcagc aatggacctc
     1981 gaccgggacc gtcccgtcga tcaacctcgg cgggcccttg tgcctgggtg gcaaccctgc
     2041 catcaacgag gagtttttcg atggcaacat tgacgaggtc gccatctact cgagtgccct
     2101 cacggcggac caggttcggc tgcatcacga ggtgggccgc gccaacatcg ccctcccgcc
     2161 ggttccggcg tcgattgttc aaggtcccgc gtccacctcc gcctatgccg gccgcatcgc
     2221 cacctttgcc gtcaacgccg atggaacctc gcctctggcc tatcaatggc acaggaacga
     2281 ctcgccgatc gacggagcga ccaacgacat cctctctttc acctgcgcgt atgccgacaa
     2341 cggcgcgacc tacaaggtgg tggtcaccaa cccctacggc tcagccacaa gcgcccccgc
     2401 caccctcacc gtcatgacgg atctgaccgt cgagtcgcaa cccgcctcga tcacccgcac
     2461 agccgggagc aaggcggctt tcatcgccgt gtccggcggc gcgctgcccg tcacttacca
     2521 gtggtacaag gcggccgacc cgattccagg cgcgacggac caaaccttgt ggctgtcgag
     2581 catccagctc gccgatgatc agacgaccta ctccgccaag atcaccaacc cctggatgag
     2641 cgtcgagacg gacccggccg tgctcaccgt ggttccgcgg gctgtgccgg tgcccgtgga
     2701 cggctatgcc cgcgtggtga tggcggatga cccggtcgca ttctggcgcc tggacgaggc
     2761 cgatgcgagt gtgacagcgg tggacgctgc cggcagcttc gatgggcaat acacgctcgg
     2821 gtcaggggcg atcacctacg gggttgcccc gggcattccg caggaaacca acccggccgt
     2881 cgccgtcacc ggcaaggccc atgtccagat tccctgggcg ctcgaactca acccgcacgg
     2941 gccgttcacc gtcgagttat ggttccaacc cgcctcgctc aacccgggcc ccgatttccg
     3001 caacgtcatt tcgtccgagg gcgccggccc caattggaac ggtccgaacg gctggctgct
     3061 gtatcatatg cccgacgaca cgctttcctg ggtgctgttc agccgagggt gggacgggaa
     3121 gtggctgggc ggcggcggcc cggtcgaggc cggcacctgg tatcatgccg tcttgactta
     3181 cgacggcgcc ctctttcaca cctatctgaa tggagacccc atcgcaacgc aggcctaccc
     3241 cgctttcgtt ccgaatgggg atggatttac cagcctggga ttccgctttg acggcaacgg
     3301 cgccggtttt gaaggcgcga tcgacgaggt ggccttctac cgcaaggcac tctcgcccga
     3361 ccagattcgc gcccactcga gcgccagcgt gaagctcagc atcgagaggt ccggggacag
     3421 catcgtcctc gcctggccct ttggcacgct gcaacaggcc gaccgcatcg acggcgcgtt
     3481 cgctgatctg acggcggcga cgtccccctg gacggtcgcc atcggcgggg ccgccaagta
     3541 ctatcgcgtg cgaatcgagt agtccttctc ggtgaggaat ccaggttggc cgggccggga
     3601 tatcaatgtc ccggcccggt tcgttcttcg cgcctgttca tgggcggcgc accgggttca
     3661 caaggatggc tgggccaaga ccacgcgaaa accgagaaat gaatggcgtg ccaccggtgg
     3721 atagccggcg cgccaactgc agcggcagtt cacccacgga aaggaccagc cgcctccgcg
     3781 gacagcgcga gtcggaccag tgatcgtgcc gatcaggtcc gccaccggct cagggggcga
     3841 agactcgatc cagtcccaac accattcgaa gacgtttccg tggacgtcct cgagtcccca
     3901 ggaattgggg gcgagttcgc caaccgggtg cgggcgcaat ccgctgttgc cgctgtgcca
     3961 gccgtacgcg ccaagcagcg ccgggtcgtc accatagcag aacgcactgc tcgtgccggc
     4021 gcgcgcggcg tactcccatt ccgcctcgga aggcagccgg tagatgaagc cgtccggcaa
     4081 gcgcccggcc tgccgctcct gctgcgtgag ccgtgcgcag taatccatcg cctcgtgcca
     4141 tgaaacccgc tcgacaggac agtcgagatt cgtggcgtac gcggcgggat ttgtgcacat
     4201 caggttttgg tattcggact gcgtgacctc gtggcggctg atccaaaagc cgcgtgtgaa
     4261 gcccgcccga aactgaccca actcgcccgc tgtgcgattc gtgtcttctg cgggcgtgcc
     4321 catgacaaac tccccggccg gcacccagcg catcgaatcg gccatttcca gacgcgacag
     4381 ccgctgcact cggtagaaac gggtccggtg cggcgcggga gaggtgtccg ccaggaacgc
     4441 gcccgtctga ttcgttgtga tcggtgcatc gacgggcgtc cacacaccga gaagatcggt
     4501 catggactgc agttgaaacc gatccccagc gtttccctcc cagatcagcg tcaactgctc
     4561 gccgcggagg cgggcggcga ttgtcggaga cgcccaggga tcgtagtccg cactcacaaa
     4621 ggcagtctgg taatccgcgt acaatctctg catgccctcg cgccgcgcct ggttccagtg
     4681 gctgagaaaa gcaaccggat tcatgtagtg caggaagtca aacgtcacga agcgctcgac
     4741 gtaaggggcg gtggcgtcga attgtcttcg caggcggagc gcatcgcatg gcatgtacac
     4801 gccgtccgaa atccggaagg attcgagatt cgcccaaaac cggacgcctg cggcgtcgca
     4861 ggcgttcttg aaggcctggg catagggcgc cacgcgttgc acaatgtcgg tctcccattg
     4921 ctgcgcgccc acgctgtctt gcaacaggag gagaccgatg ccggtttggt cgagcaactc
     4981 ccggtagacc ttctcgactc ggcagggaga cgggcgctgg ttgctgatga atggcgagat
     5041 tgccagcggc gcgtcccccg aaatgaccgc gcaggcggcg gcgatgccgc ggaagaacga
     5101 cttcagccgc gcgacttcgg ccggctggta gtccgccgtc cagggttcat aaggaatgta
     5161 ccagccggca aacgagcggt gcgggaggcc ggcctggtag cgcgcccagg cgcgttgcgc
     5221 ggtggacgcc attctcccct gggtttgagc caggaactcg ccccccgtca tgtcgtggtt
     5281 ccaattcggc aggtacagcc cgagatagac ctcgaaatca tgggcgtcgg cgtagtcgag
     5341 aatgatctcg gtcgcgtcac gcaggccgga ggagccaatg aaggaatgcg tcgtgccatc
     5401 atgattctcc cgctcgagca tctggataat gatggtgttc agccggagat cccgcatccg
     5461 gtcaagaacg gcatgccacg tttccggggc ccaggtttga agctcactct gatactgcac
     5521 gaaactccca gccagacgaa ggggcgtcgg tgccgccgca gagaccggcc aacacccggg
     5581 tgcaagggcc aacccggcaa gcaacatgga tatggtgcaa cgcatatttg ctcggtcgaa
     5641 accgatgcgg gggtgatcat gcgccaccag tgccgctgtg gcaacgtcag gcgcgaccgg
     5701 ctcgccctaa tccggagcac gatggaatgt cagactcgag aggccagcca ccgccaatga
     5761 ccgactgtcc ggcgaaaagc cgcaggcgtt catgcagcgg aagtcacaag ttccagagac
     5821 acgcgggcca gtccggcaag cgatgt
//
LOCUS       DAOQRO010000231         5824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_13942_length_5824_cov_4.475992, whole genome shotgun sequence.
ACCESSION   DAOQRO010000231 DAOQRO010000000
VERSION     DAOQRO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13942_length_5824_cov_4.475992"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKK20_09155"
     CDS             <1..231
                     /locus_tag="PKK20_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNV00093.1"
                     /translation="DVEELLSRSTDRLSRQTEIRRLLADTQGRPNISSLARQFKVARR
                     TIIRDLQELIRRGEVPPEIYPKWESSEENGLD"
     gene            complement(343..762)
                     /locus_tag="PKK20_09160"
     CDS             complement(343..762)
                     /locus_tag="PKK20_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNV00094.1"
                     /translation="MKSQTDYLWFDVPKRRGYLRITDQVETLVARSGVQEGLCLVNAM
                     HITASVYINDAEDGLLHDYDVWLEQLAPHAPTDQYHHNRTGEDNADAHLKRQIMGREV
                     VVAITKGKLDLGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            1045..1335
                     /locus_tag="PKK20_09165"
     CDS             1045..1335
                     /locus_tag="PKK20_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00095.1"
                     /translation="MNMTKPQMPTKPAPQAASGNNRPAQTIRLGAIKAAIWENTFGDT
                     TRYSVSLCRLYKDGDQWKTTESFGRDDLLLLAKVADQAHTWICEQHRPSERA"
     gene            1900..3195
                     /locus_tag="PKK20_09170"
     CDS             1900..3195
                     /locus_tag="PKK20_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNV00096.1"
                     /translation="MRYTPPDPQLFVSNRGRLTGLLQPNSIVIVNANDVLPTNADGTI
                     PLRANSDLFYLTGIEQEESLLLLCPDADDVKEREMLFVRESNPLLETWEGHKLSKDEA
                     RKLSGIDQVHWLSEFPKRFHRLMCESEHVYLNSNEHKRAVIEVETREARFVADCQRRY
                     PLHDYQRLARLMHRLRIVKSPPEIEIIRKASEITEQGVRRVLRFVKPGVTETEIEAEF
                     AHEFIRRNGNFAYPPIIATGGNACVLHYLENSAQCRAGELLLLDVAAGYANYNSDLTR
                     TIPVSGRFTRRQRQVYNAVLRVFRRCVQGLVPGKRVKEWQKEAEQMIEKELVHLGLLT
                     TRQIRQQNPANPAYRRYFMHGVGHPIGLDVHDVGFTTEPMQPGWVMTVEPGIYIREEN
                     LGVRLENTIVIGEESNIDLMSTIPLEADEIEAAMARRGR"
     gene            complement(3326..>5824)
                     /locus_tag="PKK20_09175"
     CDS             complement(3326..>5824)
                     /locus_tag="PKK20_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV00097.1"
                     /translation="IVGAAWGAGTDYGEIQARPAPVYRCPGLPCTRPNMFNISVAVHF
                     DATATQLDTIRDLIRDGSAVLFDVTDGQAEIGEAVIYNNAFGTDADLRVYPSSSPTWW
                     YANTGSYRVGGSAHVSYNYISSDSNPGPKLAHEFVHLIFDARDEYESRAVGCGDVLGA
                     AHCPDAAAQTAGEESCLMDHNGTELCWGHGDPSNLTDISGGNHDATLVTEQSRCRSNR
                     SCWEQVVWSWPDHFTMPPGAPDPDAGGAVVDPTHFVLADATVRVVLVLDESGSMSAES
                     PSRMERLKVAAKDFVTLAENGTELGIVSFSDDAEPANGHASVSIAALGAVRTAWHNAI
                     DALSPGEWTNIGDGLDKARAMIMAAGGVTANTFIVLMTDGINNRPQDDPAGHLNAALA
                     ALLTERIPVYVTCTGGDLGLSSQCSEIAAATGGFYVDSADAADLPQAFTELHEKISGR
                     EAINSMTGSLMKEPEPKTVLVEDGSESVTFALVWDDKKAGATFTIKDPLGNEYEGLPM
                     PQGRYLRRLKPAPGKWQMLVKARQPVDSRFIARGFVRHQIQSLTAAVRYPRVLPGEAI
                     HVYAYPRSIGGAITHPKETITARVLLPNGEQDGLELRDDGQGGDDLMGDGIFTGVFRN
                     TKVKGPYRFHLQAAINKWFQSTDRFRYDAEFMSPAFTREVRISAAVGEPGDTRFLAIV
                     DVTGCTLKGALVTFSIPVTPASARVPGNYSIKGLEVVGARVLSPTRVRLETSLQRPKT
                     QYTLVASNVRDTAGNRIDVPGNAFTFTSSEECVTLEFTRIVSRSGAVTVEWTGGGTLQ
                     VAPSVYGPWQDVPRASTPHKFEPAARQQFMRLRK"
BASE COUNT         1047 a         1883 c         1826 g         1068 t
ORIGIN      
        1 gatgtcgagg agctgttgag ccggtcgacc gatcgcctca gccgccaaac cgaaatccgg
       61 cggctgcttg ccgacacgca ggggcggccg aacatttcga gcctggcccg ccaatttaaa
      121 gtcgcccgcc gcaccatcat ccgcgatctg caggaactga tccgtcgcgg cgaagtcccc
      181 cccgagatct atcccaagtg ggagagcagc gaagagaacg gactggatta gcctcggagc
      241 gtcacgcgct acccgttggg acgctgggca cgccgatggc atggcgatcc gtgcatccga
      301 gaatccggtg ccgggacgag gatgcggctt tccgggctgt ggctattcgc cgatgatttt
      361 gacaagcacg cgtttgcgcc gccggccgtc aaactcgccg tagaaaatct gttcccacgg
      421 gccgaggtcg agcttgcctt tggtgatcgc caccaccact tcgcgtccca tgatctgacg
      481 cttgagatgg gcgtcggcgt tgtcttcgcc ggtgcgattg tgatggtact ggtccgtcgg
      541 cgcgtgcggc gccaactgct cgagccagac atcgtagtcg tggagcagtc cgtcctccgc
      601 atcgttgatg tagacgctgg cggtaatgtg catggcattg acaaggcaca ggccctcctg
      661 cactccgctt cgggccacca gcgtctccac ctggtccgta atcctcagat aaccacggcg
      721 tttgggcaca tcgaaccaga gataatcggt ctgtgatttc acgggcgtga ttggaaaggc
      781 gctccgggct tttgccaact ctaaaagacc acccgcgccg ggcctcgagc attcggtcca
      841 gggccgctcc ggcggattgc gtggggttcc cgggcggacg gcatccgggc agcgcatccc
      901 ttgacgacac gtgcccggtg catatcttac cggcacggcg cgccggggag cgtctttcat
      961 gcaactgcgg aaacacagcg cgcgtgcgct gtgttggaga ccgcgttcgg gccttccccg
     1021 cccgccgcat caaggaggaa cgacatgaac atgaccaaac cgcagatgcc gaccaaaccg
     1081 gccccgcagg ccgcttccgg caacaacagg ccggcccaga ccatccgcct cggcgcgatc
     1141 aaggcggcaa tctgggagaa cacgtttggc gacaccaccc gctacagcgt ctcgctgtgc
     1201 cgcctctaca aggacggcga tcagtggaaa accaccgaga gcttcgggcg cgacgacctg
     1261 ctgctgctcg ccaaagtggc tgaccaggcc cacacctgga tttgcgagca gcaccgcccg
     1321 tccgaacgcg cctgaaagac tgctcccccc cgggttggcc cgaccacacc tcctctcgat
     1381 gcatgtggag tgtccgggcc agacgggggg cagccgttct cgttttggtt ccgatcaagc
     1441 gcaccctttt gcgcttacac tgagcgccgg gccagtgtct ctggaggttg tcggtgagaa
     1501 tcgagtgtgc gctgcgtggg ggcctgcctg ccgcgcctgc ctgtgccgtg cccgacacgg
     1561 cagacaggcc gctgcgcttt gcggcgcagg cagggccccg tcgccggcaa ataccgtctt
     1621 cactcgcgtt ggccggggac gagccgtccc cgctccggcc tttgcggctt catgaattgg
     1681 cgatgcaccg ttttgtgatg acttcgagat tgactggcag atcatttggt gcgattgccg
     1741 ccaactgaaa cccgcatatg cacaggtcca gaacgcaatc cgcatcgtgt gacctggtac
     1801 tctcgagatt tcacatccct ccgctaccgc actcgagctt gtttctgtct ctcgccgcgc
     1861 gcagagtcac tgggcgtgaa tctcaactga ctcaagacca tgcgttacac gcctcccgat
     1921 ccccagttgt ttgtgtcgaa ccgcgggcgc ctcacggggt tgctgcagcc gaattcgatt
     1981 gtcattgtca acgccaacga cgtgctgccg accaacgccg acggcaccat cccgctgcgc
     2041 gcgaactcgg acctcttcta cctcaccggc atcgaacagg aggagagcct cctgctgctc
     2101 tgcccggacg cggatgacgt caaggagcgc gagatgctct ttgtgcggga gtcgaatccg
     2161 ctgctcgaga cgtgggaagg gcacaaactg agcaaggacg aggcgcgcaa actttcgggt
     2221 atcgaccagg tgcactggct gtccgagttt cccaagcgct ttcaccggct catgtgcgag
     2281 agcgagcacg tttacctgaa ctcgaacgag cacaagcgcg cggtcatcga ggtcgagacg
     2341 cgggaggcgc gtttcgtggc cgattgccag cggcgatacc cgctccacga ttaccagcga
     2401 ctggcccggc tgatgcaccg gctccgcatc gtcaagtcgc cgcccgagat cgagatcatc
     2461 cggaaggcct ccgagatcac cgagcagggc gttcggcggg tgctgaggtt tgtcaaaccc
     2521 ggcgtgacgg agacggaaat cgaggcggaa ttcgcccacg agttcatccg gcgaaacggg
     2581 aacttcgcct atccacccat cattgcgacg ggcggaaatg cctgtgtgct ccattacctc
     2641 gagaactcgg cccagtgccg cgccggcgag ttgctgctgc tcgatgtcgc cgccggatac
     2701 gccaactaca actcggacct gacgcgcacg attccggtca gcggccggtt cacaaggcgc
     2761 cagcggcagg tctacaacgc ggtgctgcgc gtcttccgcc gctgcgtcca ggggctcgtg
     2821 ccgggcaaac gggtgaagga gtggcaaaag gaagcggagc agatgatcga gaaggagttg
     2881 gtgcatctcg gcctgctgac cacgcgacaa atccggcagc agaatccggc caatccggcc
     2941 tatcggcgct atttcatgca cggcgttggc catccgatcg gactcgatgt gcacgacgtc
     3001 gggttcacca ccgaaccgat gcaaccgggt tgggtgatga ccgtcgagcc gggaatttac
     3061 atccgcgagg aaaacctcgg cgtccgcctc gagaacacga tcgtgatcgg cgaggagagc
     3121 aacatcgacc tgatgagcac gatcccgctc gaagccgacg agatcgaagc cgccatggcg
     3181 cggcgcgggc gctgaatcat cgctcggtct ttcgaggacg gggtcgttga ccaccactcc
     3241 tgaaagcctg cagggtgggt gcccttgcgc ggcaggactt ctggtgccgt gatcgagaaa
     3301 agcaaaaccg cattcggccc cgcgtctatt ttcgcagccg catgaactgc tggcgggctg
     3361 ccggctcgaa cttgtgcggc gtgcttgctc tgggcacgtc ctgccacggg ccatacacgc
     3421 tcggggcgac ctgaagcgtg cccccgcccg tccattccac cgtgacagcg ccgcttctgc
     3481 tcacgatcct cgtgaattcg agcgtcacgc actcctcact cgatgtgaag gtgaacgcgt
     3541 tgcccgggac gtcaatcctg ttccccgcgg tgtcgcggac gttcgacgcg accagcgtgt
     3601 attgggtctt gggccgttgc agactggtct cgagccggac ccgtgtcggg ctcaagaccc
     3661 tggcgccaac aacctcgagg cccttgatgc tgtagttgcc aggcaccctc gcgcttgcgg
     3721 gcgtcacggg aatcgagaac gtgacgagag ctcccttgag cgtgcatccc gtgacatcca
     3781 caatcgccag gaacctggtg tcgcccggct cgcctacggc ggccgagatc ctgacctcgc
     3841 gggtgaacgc aggactcatg aactcggcgt cgtacctgaa acgatcggtg gactggaacc
     3901 atttgttgat ggcggcctgc agatggaaac ggtaaggccc tttcaccttt gtgttgcgga
     3961 aaacgcctgt gaaaatgccg tcccccatca ggtcatcacc gccctggccg tcgtcgcgca
     4021 actcgagccc atcctgttcg ccgttgggca gcaggacgcg ggccgtgatg gtttccttgg
     4081 ggtgggtgat cgcgccgccg atgctccgcg ggtaggcgta cacatgaatg gcctctccag
     4141 gcagcacgcg cggatagcgc accgccgccg tcaggctctg aatctggtgc ctgacaaagc
     4201 cacgcgcgat gaatcggctg tccactggct gccgggcctt caccagcatc tgccatttgc
     4261 caggcgcggg tttgagtcga cgcaggtatc gcccttgggg catcggaaga ccctcatact
     4321 cgttgcccag ggggtccttg atcgtgaacg tggcgcccgc cttcttatcg tcccagacaa
     4381 gggcaaaggt caccgactcc gacccgtcct cgacaagcac cgtcttcggc tcgggctctt
     4441 tcatcagcga gccggtcatc gagttgatgg cttcccggcc cgagatcttc tcatgcaact
     4501 ccgtgaaggc ttgcggcaaa tcggctgcat ccgccgagtc gacgtagaag ccgccggttg
     4561 ccgccgcgat ctcggagcac tgagacgaga ggcccagatc gccgccggtg caggtcacat
     4621 acacggggat gcgctcggtc agcaatgcag ccaacgcggc gttgaggtgg ccggcgggat
     4681 cgtcttgcgg ccggttgttg atgccgtcgg tcatcaacac gatgaacgtg ttcgcggtca
     4741 cccctccggc ggccatgatc atggccctgg ccttgtccaa accgtcgccg atattggtcc
     4801 attctccggg gctgagggcg tcgatggcat tgtgccaggc ggtgcgcacg gcccccaggg
     4861 ccgcaatcga cacgctcgca tgcccgttgg ccggctccgc gtcgtctgaa aacgacacaa
     4921 tccccaactc ggtcccgttc tccgccaggg tgacaaagtc cttggccgca actttgagac
     4981 gttccatgcg ggacggggac tcggcgctca tgctgcccga ttcgtccagt acgagcacca
     5041 cccgcaccgt ggcgtcggcc agcacgaaat gcgtcggatc cacgaccgcc ccgccggcat
     5101 ccggatcggg agcgccgggc ggcatcgtga agtggtcggg ccacgaccag accacctgct
     5161 cccagcagga gcgattcgag cggcatcgcg actgctcagt caccagggtc gcgtcatggt
     5221 tgccgcccga gatgtcggtg aggttgctgg gatcgccgtg tccccagcac aactcggtgc
     5281 cattgtggtc catgagacac gactcttctc ccgcggtctg cgccgcggcg tcagggcagt
     5341 gagccgcgcc gaggacgtcg ccgcagccaa ccgccctcga ttcgtattcg tcgcgcgcat
     5401 cgaaaatgag gtgaacgaac tcgtgggcca gtttcgggcc gggattggaa tctgaactga
     5461 tgtagttgta ggagacgtgc gcgctgccgc ctacccggta ggacccggtg ttggcatacc
     5521 accaggtcgg gctcgaactg ggatacaccc gcagatcggc gtcggtgccg aaggcgttgt
     5581 tgtagatgac cgcctcgccg atttcggcct gcccgtccgt cacatcgaac aaaacggccg
     5641 atccgtccct gatcaggtcc cggatggtgt caagctgggt tgccgtggcg tcgaagtgca
     5701 cggccacgga gatgttgaac atgttgggcc gtgtgcacgg gagaccagga catcggtaga
     5761 ccggggccgg gcgagcctga atctcgccgt agtcggtccc tgcgccccag gccgcgccga
     5821 caat
//
LOCUS       DAOQRO010000232         5806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14006_length_5806_cov_4.360459, whole genome shotgun sequence.
ACCESSION   DAOQRO010000232 DAOQRO010000000
VERSION     DAOQRO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14006_length_5806_cov_4.360459"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..573)
                     /gene="rplF"
                     /locus_tag="PKK20_09180"
     CDS             complement(37..573)
                     /gene="rplF"
                     /locus_tag="PKK20_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNV00098.1"
                     /translation="MSRIGRLPVVVPPKVKVEVQGRTLQVEGPKGKLHVELPPRTRVA
                     LEGAQIKVSRDGEDAAAKALHGLSRSLIQNAVQGVHEGYVKKLEIHGVGFKAAVQGKQ
                     INMALGYSHQINYPLPEQIKVTVDENTKITIEGPDKQVVGQVASELRGFYPPEPYKGK
                     GIRYAGEKIVRKEGKTVQ"
     gene            complement(586..972)
                     /gene="rpsH"
                     /locus_tag="PKK20_09185"
     CDS             complement(586..972)
                     /gene="rpsH"
                     /locus_tag="PKK20_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNV00099.1"
                     /translation="MTDPIADLLTRIRNANHALLPAVEMPHSALKESIARLLKQEGYL
                     ADYSAEGTTVKKLKLKLKYQGRKGVIEGLRRVSTPGLRRYVGASEIPRVLGGMGIAIL
                     STPRGVLTGTEARRQNVGGEVLCYVW"
     gene            complement(977..1246)
                     /gene="rpsN"
                     /locus_tag="PKK20_09190"
     CDS             complement(977..1246)
                     /gene="rpsN"
                     /locus_tag="PKK20_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006791144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNV00100.1"
                     /translation="MSKLAWIERDKRKRAAVKKHAALRAELKAKKDYAALAQLPRDAS
                     PTRVVNRCKVTGRRHAHLRKFGVSRLTFREMALAGLIPGVTKASW"
     gene            complement(1248..1793)
                     /gene="rplE"
                     /locus_tag="PKK20_09195"
     CDS             complement(1248..1793)
                     /gene="rplE"
                     /locus_tag="PKK20_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNV00101.1"
                     /translation="MKPRLQQKYMDEVRPALQQKRAYINAHQIPRMVKIVIHMGVSSA
                     LEKTAIDDAAKDLGSITGRKPVIDKSRKSVANFKLRQGQPIGCHVTLRREVMYEFFDR
                     LVSTALPRIRDFRGLTPRAFDGRGNYSLGLDDQTIFPEIDLDKIKRHQGMDVTIVTTA
                     QTDAEALELLKLMGMPFAEGK"
     gene            complement(1802..2077)
                     /locus_tag="PKK20_09200"
     CDS             complement(1802..2077)
                     /locus_tag="PKK20_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNV00102.1"
                     /translation="MMQRFHVKRGDQVTVIAGTEKGKQGKVIAVLPRKQRVIVEGIKI
                     IKRHTRKSQQHPEGAIVKREGSLHISNVMRRDVFEARAAKRTAGSAA"
     gene            complement(2080..2445)
                     /gene="rplN"
                     /locus_tag="PKK20_09205"
     CDS             complement(2080..2445)
                     /gene="rplN"
                     /locus_tag="PKK20_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNV00103.1"
                     /translation="MLQVRSILEVADNSGARRAAAIGVLGRNQRYARVGDVIKAHVKE
                     ATPDGTVKKGEVVEAVVVRTRKAIHRSDGSYLRFDSNAIVIIDKEQNPRGTRIFGPVA
                     RELRDRKFMKIISLAPEVI"
     gene            complement(2452..2757)
                     /gene="rpsQ"
                     /locus_tag="PKK20_09210"
     CDS             complement(2452..2757)
                     /gene="rpsQ"
                     /locus_tag="PKK20_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNV00104.1"
                     /translation="MPETSASRNHRKERVGQVIGNRMAKTIVVRVERRIRHPRFKKVL
                     TRFKKLYAHDEQSAARLGDRVRIAETRPLSKLKNWRLVEILDRHEPIEPGAGSAAEV"
     gene            complement(2768..2965)
                     /gene="rpmC"
                     /locus_tag="PKK20_09215"
     CDS             complement(2768..2965)
                     /gene="rpmC"
                     /locus_tag="PKK20_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNV00105.1"
                     /translation="MKMSELKEKTAVELAALSRDLRQDLFNLHLQKASSQLEKPARLR
                     TLRRDIARVETQLTALKKKSA"
     gene            complement(2982..3401)
                     /gene="rplP"
                     /locus_tag="PKK20_09220"
     CDS             complement(2982..3401)
                     /gene="rplP"
                     /locus_tag="PKK20_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNV00106.1"
                     /translation="MPMMPARVKYRKQQRGNRRGLATRGNAVAYGEYGLQALERCWLD
                     TKQIEAARVAISRFMKRRGKMWIRVFPDKPITKKPLEVRMGTGKGAVESWVAVIKPAA
                     VLFEVDGVPEAVARESLRLAATKLPIQTKFISRHRLG"
     gene            complement(3414..4088)
                     /gene="rpsC"
                     /locus_tag="PKK20_09225"
     CDS             complement(3414..4088)
                     /gene="rpsC"
                     /locus_tag="PKK20_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNV00107.1"
                     /translation="MGQKTNPVGLRIAVSKDWLSKWYAGKKEFGRLLVEDKTIRGILK
                     KKLESAAVPRIQIERAANRCRVTIYTARPGVVIGRKGAEIDKLKEDLSRLTGKEIYVD
                     IQEIKDPDLDAQLVAENVALQLERRISFRRAMKKAVQTAMDKGADGIKIRCAGRLGGA
                     ELARTESYHEGRVPLHTLRAKIGYGFAEANTMYGKLGVKCWICRGEMKVEKRPATVAA
                     ASERVI"
     gene            complement(4093..4425)
                     /gene="rplV"
                     /locus_tag="PKK20_09230"
     CDS             complement(4093..4425)
                     /gene="rplV"
                     /locus_tag="PKK20_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNV00108.1"
                     /translation="MEVHAITRNVRMSPQKMREVVRQIQGMPAERAMAVLAVVPRKSA
                     RLVAKTLKSAIANAENNKNLKTGTLRVKEAVAGRAGILRRFTPKARGSPGPVLKRNCH
                     IKIVLSDH"
     gene            complement(4437..4718)
                     /gene="rpsS"
                     /locus_tag="PKK20_09235"
     CDS             complement(4437..4718)
                     /gene="rpsS"
                     /locus_tag="PKK20_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNV00109.1"
                     /translation="MGRSIKKGPFIDHHLMEKIVKAKDAKSKAPIKTWSRRSTITPEF
                     VGLTFNVHNGKIFSPVFVTENMVGHRLGEFSPTRVFRKHGAHTAKAEAK"
     gene            complement(4732..5586)
                     /gene="rplB"
                     /locus_tag="PKK20_09240"
     CDS             complement(4732..5586)
                     /gene="rplB"
                     /locus_tag="PKK20_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNV00110.1"
                     /translation="MPVKTFRPLTPSTRYATVADYREITKTKPEKRLVVTRKKTGGRN
                     CYGRITARGIGRGHKQKIRLVDFKRSKRGVAATVEAIEYDPIRSARLALVKYADGEKR
                     YIVAPNGLKVGAQVVSGPDAAPEVGNSLPLRSIPVGLPIHAIELVPGRGAQLVRSAGG
                     EASVMSRDQGYALVRLPSGEIRRIHEACYATIGQVGNLDHENIVLGKAGRSCHRGRRP
                     ITRGVARNPIDHPNGGGAGKSKSGGGWQQLTSPWGLLAKGYKTRVRSKYSNRFILVRR
                     DGRPMKLK"
     gene            complement(5596..>5806)
                     /locus_tag="PKK20_09245"
     CDS             complement(5596..>5806)
                     /locus_tag="PKK20_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNV00111.1"
                     /translation="QYTVVADPRASKTQIRRAVTELFKVKVVGIQTLNVRGKARRQRT
                     HQAGRSPSWKKAIVTLKKGDKIVLT"
BASE COUNT          935 a         1913 c         1699 g         1259 t
ORIGIN      
        1 taagccgtgc agcttccgtc agttcccgag cggcgtttac tggacggtct tgccttcctt
       61 gcggacgatc ttctcgccgg cgtaccggat tcccttgccc ttgtaaggct cgggcgggta
      121 gaacccgcgc agttcggatg ccacctggcc gaccacttgt ttgtcggggc cttcgatcgt
      181 gatcttggtg ttctcgtcga cggtgacctt gatctgctcg ggcaaagggt aattgatctg
      241 gtgcgagtag cccagcgcca tgttgatctg cttgccctgc actgccgctt tgaaaccgac
      301 tccgtggatt tcgagtttct tgacgtagcc ctcgtggacg ccctggaccg cgttttggat
      361 gagggaccgg ctcaatccgt gcagggcctt ggccgcggcg tcctcgccat cgcgcgacac
      421 cttgatctgg gcgccttcga gggccacccg ggttcgcggc ggcaactcga cgtgcagttt
      481 gcctttcggt ccctcgactt ggagcgtgcg cccctggacc tccaccttca ccttcggagg
      541 caccaccacg ggtaaacggc caatccttga cataaaaacc tcgctctacc aaacgtaaca
      601 caacacctcg ccgccgacgt tctgccggcg cgcctcggtc cccgtcagca cgcctcgagg
      661 cgtcgagagg atcgcaatcc ccatcccacc caggacgcgc ggaatctcgc tcgcgcccac
      721 atagcgcctt agcccgggcg tgcttacccg gcgcaatccc tcgatgacgc ccttgcggcc
      781 ctggtacttg agcttgagct tgagtttctt gacggtcgtt ccctcggcgg aataatcggc
      841 gaggtatcct tcctgtttga gcaaacgggc gatgctctcc ttaagggcgg agtgcggcat
      901 ctcgacagcc ggcagcagcg cgtggttcgc gttgcggatg cgggtgagca aatcggcgat
      961 gggatcagtc atacgcttac cagctggctt tggtgacgcc ggggatgagg cctgcaaggg
     1021 ccatttcgcg gaatgtcaag cgcgagacac cgaacttgcg gaggtgggcg tgccggcgcc
     1081 cggtcacctt gcagcggttg acgacgcggg tgggactggc atcgcgcggc agttgtgcca
     1141 gggcggcgta gtccttcttg gccttgagtt cggcccgcag cgccgcgtgc tttttcacag
     1201 cggcccgctt ccgtttgtca cgctcaatcc aggcgagttt cgacatatca ttttccttcc
     1261 gcaaacggca ttcccatcag tttcagcagc tcaagcgcct cggcatcggt ctgcgcagtg
     1321 gtcacgatcg tgacgtccat cccctgatgc cgcttgatct tatcgagatc gatctccgga
     1381 aaaatggtct ggtcatccag acccaacgag tagttgcccc gtccgtcaaa cgcccgcggc
     1441 gtcaagccgc ggaaatcccg aatgcggggc agggctgtag acaccaggcg atcgaaaaac
     1501 tcgtacatca cctcgcggcg gagagtgacg tggcatccga tgggctggcc ctgccgcagc
     1561 ttgaagttcg cgacgctctt gcgcgacttg tcgatcaccg gtttgcgtcc ggtgatcgat
     1621 ccgagatcct tggcggcgtc atcgatggcg gtcttctcca gcgcggagct gacacccatg
     1681 tggatgacga tcttcaccat ccgggggatc tggtgggcat tgatgtacgc gcgcttctgc
     1741 tgcagcgccg ggcgcacttc gtccatgtat ttctgttgca gtcttggctt catggctggt
     1801 ctcaggcggc cgaaccggcc gtccgcttgg cggcccgggc ctcgaacaca tcgcggcgca
     1861 tcacattcga gatgtgcagg gacccttccc gcttgacgat ggccccttcg ggatgctgtt
     1921 gactcttgcg ggtatggcgt ttgatgatct tgatcccttc aacgatcacc cgctgcttgc
     1981 ggggcagcac ggcgatcact ttgccctgct tgcccttctc cgttccggcg atcaccgtca
     2041 cctgatcgcc ccgcttgacg tgaaatcgtt gcataatcgc tagatgacct cgggggccag
     2101 ggagatgatc ttcatgaact tgcggtcgcg caactcgcgg gccaccggcc cgaagatgcg
     2161 ggtcccgcgc gggttttgct ccttgtcgat gatcacaatg gcgttgctgt cgaagcggag
     2221 gtaggagccg tcgctgcggt gaatggcctt gcgggtgcgc accacgaccg cctcgacaac
     2281 ctctcccttc ttcaccgtcc catcgggagt ggcttccttg acatgggctt tgatgacatc
     2341 gccgacccgc gcatagcgct ggttgcggcc gagcacgccg atggcggcgg cccgtcgcgc
     2401 tccggaattg tcggccactt cgagaatgga acgtacttgc agcataacac ctcacacttc
     2461 agccgccgaa cccgcgccgg gttcgatcgg ctcgtggcgg tccaggattt ccaccaggcg
     2521 ccagtttttg agtttggaca gcggccgggt ctcggcaatc cgcacacggt cgcccaaccg
     2581 ggccgcgctc tgttcgtcgt gcgcgtacag tttcttgaag cgggtgagca ctttcttgaa
     2641 acgcggatgg cgaatgcggc gctcgacgcg cacgacgatt gtcttggcca tccggttgcc
     2701 gatgacctgg cccacgcgct ctttccgatg attccgactg gcactcgtct caggcataca
     2761 tccccactta cgctgacttc tttttcaagg ctgtcagttg cgtctccacg cgcgcaatgt
     2821 cgcggcggag cgtccgcagc cgggccggtt tctccagttg gctgctcgcc ttctggaggt
     2881 gcaggttgaa caggtcttga cgcaagtccc ggctcaacgc tgccaattcg accgccgttt
     2941 tctccttcaa ctcagacatc ttcatggtca aagcgctcct gctaccccag ccggtgccgg
     3001 gaaatgaact tggtctggat cggcagcttg gtggcggcca gacgcagcga ctcgcgcgcc
     3061 acagcctccg gcacaccgtc cacttcgaac aacaccgcgg ccggcttgat cacggcgacc
     3121 cacgattcca cggcgccctt gcccgtgccc atgcgaacct cgagcggttt cttggtgatc
     3181 ggcttgtcgg gaaagacccg aatccacatc ttgccccgcc gcttcatgaa gcggctgatg
     3241 gcgacgcggg ccgcctcgat ctgtttggtg tccagccagc agcgctcgag cgcttgcagt
     3301 ccatattctc cgtacgccac ggcgttcccg cgcgtggcca accccctgcg gtttccacgc
     3361 tgttgtttgc ggtacttgac tcgggcaggc atcatcggca tatcaaatcc tcctcagatc
     3421 acccgttcgc tggccgccgc gaccgtcgcg gggcgtttct cgaccttcat ttccccccgg
     3481 cagatccagc acttgacacc cagcttgcca tacatcgtgt tggcctctgc aaatccgtat
     3541 ccgatcttgg cccgcaaggt gtgcagcgga acgcggccct catgatacga ttcggtccgc
     3601 gccaactcgg ccccgcccag gcgcccggcg cagcgaatct tgatgccgtc cgcccccttg
     3661 tccatggcgg tctgcaccgc cttcttcatg gcccggcgga acgagatgcg gcgctccaac
     3721 tgcagcgcga cgttctcagc caccaactgg gcgtccagat cgggatcctt gatctcctgg
     3781 atatcgacgt agatttcctt gcccgtcagg cggctcaggt cctcttttag cttgtcgatc
     3841 tcggccccct tgcggccaat gaccacaccc ggacgggccg tgtagatggt gacgcgacac
     3901 cggttcgccg cccgctcgat ttggattctc ggcacggcgg cggattcgag tttctttttg
     3961 agaatgccgc gaatggtttt gtcctcgacc agcagccggc cgaactcctt cttgccggca
     4021 taccacttgg acagccagtc tttcgagacc gcaatgcgca aaccgacagg atttgtcttc
     4081 tggcccataa tgttaatggt ccgacaggac gatcttgatg tgacaattgc gtttgaggac
     4141 cggtccgggg gaacctcgcg ccttgggcgt gaacctccgg agtatccccg cccggcccgc
     4201 aacggcctct ttcacacgca aggtcccagt cttgaggttc ttgttgttct cggcgttcgc
     4261 aatggcggac ttgagggtct tggcgaccag ccgcgccgac ttgcggggga ccaccgcgag
     4321 caccgccatg gcccgctcgg ccggcatgcc ttggatttgc cgcacaacct cccgcatctt
     4381 ctgcggggac atgcgcacgt tccgggtgat ggcgtgaact tccatatgtc tgctccctac
     4441 ttggcttccg ctttcgccgt gtgcgcgccg tgcttccgga acacacgcgt cggagaaaac
     4501 tccccaagcc gatgcccgac catattctcg gtgacaaaca ccgggctgaa aatcttccca
     4561 ttgtgcacgt tgaacgtcag cccgacaaac tcgggcgtga tcgtcgatcg acgcgaccag
     4621 gtcttgatcg gcgccttcga cttcgcgtcc ttggccttca caatcttctc catcaagtga
     4681 tggtcgataa acgggccttt tttgatcgaa cgtcccataa aaaatctcag gttacttgag
     4741 cttcatcggg cggccatcgc ggcggaccag aatgaaccgg ttcgagtact tgctgcgaac
     4801 gcgcgtcttg tatcccttgg ccaggagccc ccacggcgac gtcagttgct gccacccgcc
     4861 gccgctcttg gacttgccgg ccccaccgcc gttgggatga tcgatcggat tgcgcgcgac
     4921 accccgggtg atcgggcggc gcccgcggtg gcaggatcgc cccgccttgc ccaacacgat
     4981 attttcgtgg tcgaggtttc ccacctggcc gatcgtggcg tagcacgcct cgtgaatccg
     5041 gcgaatctcg cccgacggca gccgcaccag ggcgtagcct tgatcgcggg acatgacgga
     5101 cgcctcgcct ccggccgagc gcaccaactg ggcaccgcgc ccgggcacga gctcgatcgc
     5161 atggattggc agcccgactg gaatcgatct gagcggcagg ctgttgccaa cctcaggagc
     5221 ggcgtcgggt ccggacacaa cctgggcgcc caccttgagc ccgtttgggg cgacgatgta
     5281 gcgcttctcc ccatccgcat acttcaccag cgccaaacgc gcgctgcgga tcgggtcgta
     5341 ctcgatggcc tcgacggtgg ctgccacacc gcgtttgctc cgcttgaaat ccacgagccg
     5401 aatcttctgc ttgtggcccc tgcctatgcc tcgggctgtg atccggccat agcagttccg
     5461 tcccccggtc ttcttccgcg tgacgaccag gcgcttctct ggcttggtct tggtgatttc
     5521 ccgataatcc gccaccgtcg cgtagcgggt cgacggggtt aagggtcgaa atgttttgac
     5581 aggcataacc aatccttacg tcagcacaat cttgtcgccc ttcttcaggg tgacgatggc
     5641 cttcttccag ctgggcgatc gccccgcctg gtgtgtgcgt tggcgccgcg ccttgccacg
     5701 aacgttcagt gtctgaatgc cgacaacctt caccttgaac aactccgtca ccgcccgacg
     5761 gatctgggtc ttgctcgcac gagggtcggc gaccaccgtg tactgg
//
LOCUS       DAOQRO010000233         5765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14171_length_5765_cov_4.686865, whole genome shotgun sequence.
ACCESSION   DAOQRO010000233 DAOQRO010000000
VERSION     DAOQRO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_5765_cov_4.686865"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PKK20_09250"
     CDS             complement(<1..1107)
                     /locus_tag="PKK20_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNV00112.1"
                     /translation="MIASEAVRRIIRHFRPAPAEASAKSGAPFLVVLTGNPNCGKTTL
                     FNALTGLRAKVGNYAGVTVERREGRLTGADAASPIHVLDLPGAYSLSPQSLDEQIAGD
                     VLFHRVQDLPPPDLIVVVVDASNLKRNLYYATQVVELGYPTLVALNMVDVARDNGHLV
                     DAAELQKQLGVPVFPTVASTRKGVVALRSRILSAHRGEVAPGPVPLFANLPAPFEQEA
                     AALAQLLSEKAFANRTRFARPAARLILSDEKSLVSVPALYGPVVRDSVIAARERLQAA
                     GLDWRTLLIESRYARVSAIYDAAVSESVVAEETPSDRLDRVLTHRVWGLVIFTALMAL
                     MFQSIFAFAKLPMNALEAGVAWLGFDATWVKALPT"
     gene            complement(1202..1498)
                     /locus_tag="PKK20_09255"
     CDS             complement(1202..1498)
                     /locus_tag="PKK20_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNV00113.1"
                     /translation="MVLPVSTNAAAVPPCSSRMTCPLSKVRAGTCVRIKRLLGEPAQC
                     QRLREMGFCEEQQVRLLLRNRSVVCQVCNVRFGLSAGLADSIWVEPIPPERKAA"
     gene            1918..2151
                     /locus_tag="PKK20_09260"
     CDS             1918..2151
                     /locus_tag="PKK20_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00114.1"
                     /translation="MFVRDPTWRFQRWQVVGDAHPLVAEPAGLMYDHAEVTVVEPSAE
                     GDVEFKINETLAGLTLMVNGKKIKPASQSSREV"
     gene            complement(2188..3177)
                     /locus_tag="PKK20_09265"
     CDS             complement(2188..3177)
                     /locus_tag="PKK20_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV00115.1"
                     /translation="MDHPIASAQDRRALQMAMSLSLAVGVLMFVIKVSAYLLTGSAGI
                     LSDAAESVVHVAAVVFAFVSLRLTFKPADDSHLYGHAKISFFSAGFEGGMIVLAAIYI
                     LYESIHKWILGQPLERLGLGTLLTAVAAGINGLLGGYLVWIGRRKNALILEANGKHVL
                     TDCWTSVAVLVGLALTMLTGWQPWDPICGIFMAINILWAGCSLIRSAFGGLMDQADPA
                     VQKQLETILDRETAQRGLIYHQLRHRNIGNAHSVEVHLIFPAETSLVEAHRQATAIER
                     VIEQTIEPRAQVTTHLESITDHDDLHQHSIRNPSHADGLSDRAGAHDTRVNSR"
     gene            3276..3731
                     /locus_tag="PKK20_09270"
     CDS             3276..3731
                     /locus_tag="PKK20_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNV00116.1"
                     /translation="MRNKIFPMVAALTVLFAAGCAGPEKKLGRGINNLTEFARLGEIS
                     RSVEQTFLWDGPQSAYTTGLIRGINRSLVRTGVGLYEVVTFPIPSYDPVLRPGGPILH
                     DASVNPTHAASYTPHMISSSTFEPDAALGFSGGDIAPFVPGSRFRIFDY"
     gene            3782..4669
                     /gene="ispE"
                     /locus_tag="PKK20_09275"
     CDS             3782..4669
                     /gene="ispE"
                     /locus_tag="PKK20_09275"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNV00117.1"
                     /translation="MSVERLSPCKVNLCLNVLGPRPDGYHNLETLMHPLALSDRLECE
                     RTGTRVVLTCTRSDLPVDSSNLVVRAANAFRDEAGLTDGIRMHLDKQVPMAAGLGGGS
                     GNAAAALLALNELFGFPLSPQQLARLAAQLGSDVPFFLQSKPALATGRGEIIEPLDWF
                     QALEGAWMLLVHPGFGISTAWAYQSLERFPEALHGQPGRAQRFVESLQSRTLDEASAD
                     FYNSFEGPVFEKHPILALYKEFLRESGANAALMSGSGSAVFALTTSQEAARALRDRFL
                     ARFGTSPWTEMLPVGPSCL"
     gene            5040..5113
                     /locus_tag="PKK20_09280"
     tRNA            5040..5113
                     /locus_tag="PKK20_09280"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5072..5074,aa:Gln,seq:ttg)
     gene            5214..>5765
                     /locus_tag="PKK20_09285"
     CDS             5214..>5765
                     /locus_tag="PKK20_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00118.1"
                     /translation="MSLTRRQFLRRSSVAAATLALPMAAGWRVLGANEEIRIALVGCG
                     IRGGTHVAEFGKQPGVRIVAVCDPDHERVASFAKRVESGYGNRPVEVDDVRRLMERKD
                     FDVVAVATMQYWHALPTIWACETGRHVYVEKPLAHFIWEGRQMVNAARKHNRLVQVGT
                     QGRSRGTDQQLVQYLKSGQLGKIQ"
BASE COUNT         1044 a         1927 c         1742 g         1052 t
ORIGIN      
        1 ggtgggcagg gccttcaccc aggtcgcgtc gaagcccagc caggccactc cggcttcgag
       61 cgcgttcatc ggcagcttcg cgaacgcaaa gatgctctgg aacatcagcg ccatcagcgc
      121 cgtgaaaatg acaagacccc aaaccctgtg cgtcagcacc cgatccagac gatcgctcgg
      181 cgtctcctcc gccacaacgg attcgctgac cgctgcatcg taaatcgcag acacccgggc
      241 gtaccgggat tcgatcaaca gcgttcgcca atcgagtccc gccgcctgca atcgttcccg
      301 ggccgcaatc acggaatcgc gcacaaccgg cccgtagagg gcgggaacgg acaccaggct
      361 tttttcgtcc gagagaatca gccgcgccgc cgggcgcgcg aatcgagtgc ggttggcgaa
      421 ggccttctcg gacaggagtt gagccagggc tgccgcctcc tgctcgaaag gagcgggcag
      481 gtttgcgaaa agggggaccg gccccggagc gacctcgcct cgatgcgcgg agagaatccg
      541 cgatcgcagg gcgaccaccc cctttcgcgt gctggcgact gttggaaaga ccggcacacc
      601 aagctgcttc tgcagctccg ccgcatcgac caaatgcccg ttgtcgcggg ccacatccac
      661 catgttcaac gccaccagcg tcggataacc caactcgacg acctgcgtcg cgtagtagag
      721 attccgcttg aggttcgaag cgtccaccac aaccacaatc aggtccggcg gcggcaggtc
      781 ctgcacccgg tgaaagagaa cgtcccccgc aatctgctcg tccagcgatt gcggactgag
      841 gctgtaggcc cccggcagat cgagaacatg gatcggcgac gcggcgtcgg cgcccgtcaa
      901 acgcccctcc ctccgctcca cagtgactcc ggcgtagttc cccactttcg cgcgcaatcc
      961 cgtcagggca ttgaacaacg tcgtcttccc gcaattcgga ttgcccgtga gaaccaccag
     1021 gaacggggcc ccgcttttgg ccgaagcctc ggctggggct ggtctgaaat gacgtatgat
     1081 acggcgcacg gcttcggagg cgatcatggg aggcgaggct gtctcacccg gactctgggc
     1141 acagatgcat gctcccccgc cggcaggccg gtcgagggca agaggatttt cgccaggcgg
     1201 ttcaggccgc tttccgctcc ggtggaatcg gttcgaccca gatgctgtcg gccagcccgg
     1261 cgctgaggcc gaagcggacg ttgcagacct gacacaccac gctgcgattt cgcaagagaa
     1321 ggcggacctg ctgttcctcg cagaagccca tctcgcgcag ccgctgacac tgcgccggtt
     1381 caccaagcaa tcgcttgatg cggacgcatg tgcccgcccg caccttgctg agcggacatg
     1441 tcatccggct cgagcaggga gggacggctg cggcatttgt gctcacgggc agcaccatag
     1501 cggaattgag acttggttgc aagaggcttc tcggcccggc gcttgagcca agtcaattcc
     1561 tgcgcccaaa gacgccgccg cccacgccgc cgtacccccc gtgcgccaac ccgtctttgg
     1621 gctcgaccgc tcgtctccga tggccatccc gtccgggcgt atgagtctcc ggatatgccc
     1681 ggcttggggg cctctgcggc gcattgcccc gcgactgtcc ctcctcctcc tccccgccct
     1741 cgtcgcagtc ttcgccgcgg tcatcgccct ctcggaaaca ccacccccat ccgagcccaa
     1801 tggatttggt cgcagggcgg ggacgtccac gcctataacc agactggcga agtcgctgat
     1861 ccggagtcat tccgcgaccg gctgggccgc cgggcggagt cgatacaaca agacgccatg
     1921 tttgtccggg atccgacctg gcggttccag cggtggcagg tggtgggtga cgcgcacccc
     1981 ctggtcgccg agcctgccgg gctcatgtat gatcacgccg aagtcacggt cgtggagccc
     2041 agtgcagagg gtgacgtcga gttcaaaatc aatgagactc tcgccggcct caccctcatg
     2101 gtgaacggga agaagatcaa acccgcatcg cagtcatctc gcgaagtctg accggcctgt
     2161 gcgctgacgg gcagcgaaat gtgcgggtta gcgcgagttg acgcgagtgt cgtgtgcgcc
     2221 cgcccgatcc gacaatccgt ccgcatgcga cggattccgg atgctgtgct gatgcagatc
     2281 gtcgtgatcg gtgatggatt cgagatgcgt tgtcacctgg gcgcggggtt cgatcgtctg
     2341 ttcaatgacc cgctcgatgg ctgtcgcctg gcgatgcgcc tcgacaagcg aagtctccgc
     2401 cgggaaaatc aggtgcactt ccaccgaatg cgcgtttccg atgttccggt ggcggagctg
     2461 gtgatagatc agcccccgct gggccgtctc gcgatcgagg attgtttcga gctgcttctg
     2521 caccgcggga tcggcttggt ccatcaaccc tccgaacgcg gagcggatca ggctgcatcc
     2581 cgcccagaga atgttgatcg ccatgaatat gccgcagatc ggatcccacg gctgccagcc
     2641 cgtgagcatg gtcaacgcca atcccacaag caccgccacg ctggtccagc agtcggtcag
     2701 cacatgcttg ccgttggctt cgagtatcaa cgcgttcttc cggcgcccga tccagaccag
     2761 atagcctccg agcagaccgt tgatcccggc cgccaccgcg gtcaggagcg tcccaagccc
     2821 cagccgctcg agcggctgtc cgaggatcca cttgtggatg gattcataga ggatataaat
     2881 cgcggccagc acgatcatcc ctccctcgaa tccggcagag aagaaggaga tcttggcatg
     2941 gccgtagagg tgcgaatcgt cagccggctt gaacgtcagc cggaggctca caaaagcgaa
     3001 taccaccgcc gccacatgca ccaccgactc cgccgcatcg ctcaaaatcc ccgccgaacc
     3061 ggtcagaaga tacgcgctga ccttgatgac aaacatcaat accccgaccg caagcgagag
     3121 gctcatggcc atttgcagtg ctctgcggtc ctgcgccgaa gcgatagggt ggtccacggg
     3181 ccctgagcgt accggtttct ccccgttccg gaagcccgaa aagcgcttta caaggcccgg
     3241 ctgacggata acctgatgcc caacaaacga tctttatgcg taacaagatt tttcccatgg
     3301 ttgcggcgct cacggtgctc tttgcggcgg gctgcgccgg accggaaaag aaactcggtc
     3361 gcggcatcaa caatctcact gagtttgccc ggctgggcga aatcagccgt tcggtcgagc
     3421 agaccttcct ttgggacggc ccccagagcg cctacaccac gggtctcatt cgcggcatca
     3481 atcgaagcct ggttcgaacc ggagttggcc tgtacgaggt cgtgaccttc cccatcccca
     3541 gctacgaccc cgtcctccgc ccaggcggtc ccatcctgca cgacgcgtcg gtcaatccga
     3601 cgcacgccgc cagctacacg ccgcacatga tctcctcgtc gacgttcgag cccgatgccg
     3661 ccctcggctt cagcggcggc gatatcgcgc cgttcgtccc gggcagccgg ttccgcattt
     3721 tcgactatta gccccagcat ttccccaggg cgccaacgca tctgttggcg ccttttttgt
     3781 tatgtccgtc gagcgccttt cgccctgcaa ggtcaacctc tgcctcaacg ttctggggcc
     3841 gcgccccgac ggttaccaca acctcgagac cctcatgcac ccgctcgccc tctccgaccg
     3901 gctcgagtgc gagcggaccg gcacgcgtgt cgtcctgacc tgcacccgct ccgacttgcc
     3961 ggtcgacagc tcgaacctcg tcgtccgagc ggcaaacgcg tttcgtgacg aggccggcct
     4021 gacggacggc attcgaatgc atctcgacaa acaggtgccc atggcggccg gcctgggagg
     4081 tggcagcggc aacgccgccg ccgctttgct ggcgctcaac gagttgttcg gcttcccact
     4141 cagcccgcag cagttggcgc gcctggctgc gcaactgggc tccgacgtcc ccttcttcct
     4201 ccagtccaaa ccagccctcg ccacaggccg cggcgagatc atcgaaccgc tggattggtt
     4261 tcaggccctc gagggcgcgt ggatgttgtt agtccatcca ggcttcggca tctcgaccgc
     4321 ttgggcctat cagagcctcg aacgattccc cgaggccctt cacggccagc ccggacgcgc
     4381 ccaacggttc gtcgagagcc tgcaatcgag aacgctcgat gaggcgtccg ccgacttcta
     4441 caacagcttc gagggcccgg tattcgaaaa gcacccgatc ctggctttgt acaaggagtt
     4501 ccttcgtgag agcggggcga acgccgccct gatgtcggga agcggttccg ccgtgttcgc
     4561 tctgaccacg agccaggagg ccgcgagggc cttgcgcgat cgctttctcg cgcgcttcgg
     4621 gacaagcccg tggaccgaga tgttgcctgt tgggccttcg tgcttgtgac gactcaggtc
     4681 ctgttgtttc ggagttcctg atcgcccccc aagcgtcggt gcatctgaaa gttgtcgatg
     4741 ggaatggagt ttgcgctgcg tgggggcctg cctgccgcgc ctgcctgtgc cgtgcccgac
     4801 acggcagaca ggccgctgcg ctttgcggaa cacgaagggc acacggcctt gtttgctctg
     4861 ttcgcgcaca cggaggtgtt ggagtggggc gtcgcccctt cggcagtctc catccgttgc
     4921 agtcccggcc ccctcaccga gcgacagaat accgacgaca accagatgca ccgcacttct
     4981 ggctcgcccc acttttggct ttgcgcactc gtcagctcgc cggtagctta accgcgggtt
     5041 gtccgatggt gtaaaggtag cacagaggtc tttggagcct ttagtccagg ttcgaatcct
     5101 ggtcggacag ccataaaagt aagaatccgg ttcagggaat ccgtttcacc ttgcgtggcc
     5161 tctccgggcc gtgcaaactc aggccacatc gaacacgcca atccctatta ccaatgtccc
     5221 tcacacgccg tcaattcctc cgtcgttcgt ctgtcgcagc cgcgacgctg gccttgccga
     5281 tggccgccgg ctggcgcgtc ctgggagcca acgaagaaat ccgcatcgcc ctggttggct
     5341 gcggaatccg aggcggaacc cacgtcgcgg agttcggcaa gcagccgggc gtgcgtatcg
     5401 tcgcagtctg tgacccggac cacgaacgcg tggcgtcctt cgccaaacgg gtcgaatcgg
     5461 gttacggcaa tcgtccggtc gaggtggacg atgtgcgccg gttgatggag aggaaggatt
     5521 tcgatgtggt ggcggtggcc acgatgcagt actggcacgc gctgccgacg atctgggcct
     5581 gcgagacggg ccggcacgtg tacgtcgaga agcctctggc gcatttcatc tgggagggcc
     5641 ggcagatggt caacgcggcc cgcaagcaca accggcttgt tcaggtcggc acacaaggcc
     5701 gctcgcgggg cacggaccag caactggtgc agtacctcaa gtcaggccag ttgggcaaga
     5761 tccag
//
LOCUS       DAOQRO010000234         5756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14203_length_5756_cov_4.099982, whole genome shotgun sequence.
ACCESSION   DAOQRO010000234 DAOQRO010000000
VERSION     DAOQRO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14203_length_5756_cov_4.099982"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="PKK20_09290"
     CDS             <1..1809
                     /locus_tag="PKK20_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV00119.1"
                     /translation="QDQHAGLAARLQMERRLGKRDILAHYLDRAPFGGNIVGIEAAAR
                     RYFHKGADELCLAEAALLAGLPQSPSRLRPDRHPERARERQAWVLDRMQALGMITARE
                     RAEAIAQPIQVRPGSYPFSAPHFCDLVGVPARPACGSIVRTTLDVDLQRLAEGSLRRC
                     LQGGPVDCGAVVVIEVGTGAVRALVGSPDYARPRAGQVNGAVARRAAGSTLKPFAYAL
                     ALDCGLVTPATVLTDVPVRFRDFDPRNFSLDFLGRVSVRDALVLSLNLPAIDIEQRVG
                     QERFHAILRDVGLSSISQPASHYGLGLVLGNAEVRLLDLANAYACLARGGGWAPPRFV
                     ESPNPPATRRVFSPEACWLISDMLGGEERAMDATGHAADVRLPPMAWKTGTSAGLRDA
                     WTVAWNPEVVIAVWVGNSDGSASEHLVGRRIATPIAWDLLRRLYPDNRGPWFARPAGI
                     VTRAVCAESGCAPGPHCECRGEDWAIASVSRHALCPVHREGTKAAWPPSVAAFLNRGA
                     APAAGESAGSLRILSPARGCTYRYVPDLDVDAQRIAFDAATDRAGDPLHWFVDDRPVG
                     RSRVGQPMFWPIQRGRHRIVCSTLGGASDCVQITIE"
     gene            complement(1975..5208)
                     /locus_tag="PKK20_09295"
     CDS             complement(1975..5208)
                     /locus_tag="PKK20_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00120.1"
                     /translation="MAIWAAEPGHPSPSLAPGANVDVAPWGERASWENGRDVGVWWED
                     PRDILRVVVAFAETPPPPYQARLEWWQSQWPERRIPRDQPAGAGESGWGHLGDLYQGQ
                     WLEADADIESTGTTWEYTFRPIHQKEFSQLESFKARHRTTMRLRLLFEDTAPKIERIE
                     AFTDSVWKSVEVGIEWGGEGTGERCWDGQLSVFNGYVEALAPLVSGPGGSRVEITGPA
                     QWRSSVRDLPDGVRCRVWFADSPNVNTFDSTVVTLRTASGDFSFDPAAVAKGATILSP
                     MHDAVVGPADQIALEHLRQTRSQGVGCSAFRAAAERARTNGTPTLYARVFDEPEQSLE
                     RALAEQPPKRGRMYLPVGLEGGRQRFGINPSGSVFCVNDRIDAPPGKDTVRRRWGSQR
                     LDYDFGLPRSGLTRRWIEDDCLPIQHAEWECEGIRYSQTAFATRLDPGSLGYPDTQAD
                     DTTILMVKIAGRNLAQEYKPARVALSLRADGRRVPLHLEEGLVRAKLADGEVIRALIE
                     GQGQVYPPEVDGTIQYFGNMPPGTDGFIVVKIPFITLNAPDEIERLRNLEFDREFDRT
                     RRFWRSRVAAATRITTPIPELNNFHRAHVSHLLINCGREPGANRLAARVGGFSYGVFG
                     NESCMMITDLDRRGLHGEAERCLDTFLDYQGTVCLPGDYTSQEGVFNGAFGWEQGGYN
                     QHHGWIMWAMAEHYWFTGDKPWLARHADKLVKACHWITSQRARTRNLDGLRRIERGLL
                     PPGSLEDIGDWRCWMSNNGFSWWGLNAVAGALADIHHPEAQALRAEAMAYRRDILDAF
                     REARVRSPLVALRDGTWIPQIPSEVHRRGRTFGWITVTLEGSIYFLRTGLMAPNDPMA
                     VPIMQDFEDNLYLSERYGYSWEKHGAHWFSRGGISMQPNLLCSPHPYLLRDEIKHFLR
                     AYFNAFASCYYPDTQMMCEHPLPDLGDWRGDHYKASDESNSTYWLRLMFIEDDRDDNV
                     LRLGMALPRAWLADGQSPAIERAATHFGPMSLRYESRANAASITAVLDPPRRRTPDRV
                     LLRFRHPEAKPFRRVEVNGQPWTGFDTDKEWVELPKAEHLLTIVAFY"
     gene            complement(5537..>5756)
                     /locus_tag="PKK20_09300"
     CDS             complement(5537..>5756)
                     /locus_tag="PKK20_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00121.1"
                     /translation="ACSGLMDMLAEGHHGVALSRMEFEKIACWIDLLVPFCGDYAEAN
                     TWTAEEQSKYIRFLEKRRKSEKMTVAND"
BASE COUNT          944 a         1890 c         1869 g         1053 t
ORIGIN      
        1 caggatcagc atgccggcct ggccgcgcgc ctgcagatgg agcgccgcct gggcaagcgc
       61 gatatcctgg cgcattatct cgatcgcgcg cccttcggcg gcaacatcgt cggcatcgag
      121 gccgcggccc gccgctactt ccacaaagga gccgatgaac tgtgccttgc cgaggcagcc
      181 ctgctagccg gtttgccgca atccccatcg cgtttgcggc ccgaccgaca tccggagcgg
      241 gcgagagaac gtcaggcctg ggtcctcgac cgcatgcaag ccctggggat gattacggca
      301 cgggaacggg cggaggctat cgcccaaccg atccaggtgc gccccggttc ctatccgttc
      361 agcgcgccgc acttctgcga cctcgtcggg gtgcccgcgc gccccgcgtg cgggtccatc
      421 gtccgcacca ccctggatgt cgatctgcag cgcctggccg aaggcagtct gcgacgttgt
      481 ctgcagggcg gccccgtcga ctgcggagcc gtggtcgtga tcgaggtcgg aaccggcgcg
      541 gtgcgcgccc tggtgggttc ccccgactac gcgcggcctc gcgcgggaca agtcaacggc
      601 gcggtcgcgc ggcgcgcggc gggatccacg ctcaagccct tcgcatacgc gctggccctc
      661 gactgcgggc tcgtcacccc cgccacggtg ctgacggatg tgccggtgcg ctttcgcgat
      721 ttcgatccgc ggaatttctc gctcgatttt ctgggtcggg tcagcgtgcg cgacgccttg
      781 gtgctctcgc tgaatctgcc ggccatcgat atcgagcagc gggtgggaca ggaacggttc
      841 cacgccatcc tccgcgacgt cgggttgtcc tcaatctccc aaccggcatc tcactatgga
      901 cttggcctgg tgctgggcaa tgcggaggtg cggttgctcg acctcgccaa cgcctatgcc
      961 tgcctggcgc ggggcggcgg ttgggctccc ccgcgcttcg tcgagtcccc gaatccgcct
     1021 gcgacgcggc gggtcttctc gcccgaagcg tgctggttga tcagcgatat gctcggcgga
     1081 gaagagcgcg ccatggacgc cacgggccat gccgccgacg tgcgcctgcc gcccatggcc
     1141 tggaagaccg ggacctccgc gggtctgcgc gacgcctgga cggttgcctg gaatccggaa
     1201 gtcgtcattg ccgtttgggt gggtaactcg gacggatccg cctccgaaca tctcgtcggg
     1261 cgcaggatcg ccacgcccat cgcctgggac ctgctgcggc ggctctatcc ggacaaccgt
     1321 gggccgtggt ttgcccgtcc ggcgggtatc gtgacgcgcg ccgtgtgcgc cgagagcggc
     1381 tgcgcgccgg gaccgcattg tgaatgtcgc ggcgaggact gggccatcgc gagtgtctcc
     1441 cggcacgcgc tctgtccggt gcaccgcgag ggaaccaaag cggcctggcc tccgtccgtg
     1501 gcggcgttcc tgaaccgggg cgcggcgccg gcagcggggg agtccgccgg ctcgttgcgc
     1561 atcctctcac ctgcgcgcgg gtgcacctac cgctatgtgc cggacctcga cgtcgatgcc
     1621 cagcgcattg cattcgacgc cgctaccgac cgggcgggcg atcctctgca ttggttcgtg
     1681 gacgatcggc cggtcggaag atcgcgtgtc ggacagccca tgttctggcc gatccagcgg
     1741 ggccggcacc ggatcgtttg cagcacgctt gggggggcga gcgactgcgt gcagatcacg
     1801 atcgagtgag cgagccgcgc gcctctcctt ccttccactt cgttcggcgg ccagcccgca
     1861 tgtttcatgg actagtgacc actgtgaaac caacgccccc caaaaaatgc ggaccgctgc
     1921 aggccggaac gcgcccggag atcggtgtcc tcgtcccggc atggaatctc cgggctagta
     1981 gaaggccacg atagtcaaca gatgctccgc tttcggcagt tcgacccatt ctttgtccgt
     2041 atcgaacccc gtccagggct ggccgttgac ctcgacgcgc cggaagggct tggcctccgg
     2101 atggcggaag cggagcagga cgcggtccgg cgtccggcgc cttggcgggt cgaggactgc
     2161 cgtgatcgag gcggcatttg ccctggactc gtaccgcagg ctcattgggc cgaagtgcgt
     2221 cgcggcgcgt tcgatggccg gactctggcc gtccgccagc cacgcgcgcg gcagggccat
     2281 gccgagtcgc aggacattgt cgtcccgatc atcttcgatg aacatcaggc gcagccagta
     2341 ggttgagttc gactcgtcgc tggctttgta gtggtcgccg cgccaatcgc caaggtcggg
     2401 caggggatgc tcgcacatca tctgggtgtc cggataatag caagaggcaa acgcgttgaa
     2461 gtaagcgcgc aggaagtgtt tgatctcgtc tcgcaacaag tacggatgag gcgagcagag
     2521 caggttgggc tgcatcgaaa tgccgccccg gctgaaccaa tgggcgccgt gtttctccca
     2581 cgagtaccca taccgttcgg acaaatacag attgtcctcg aaatcctgca tgatcggaac
     2641 tgccatggga tcattcggcg ccatcagccc ggtgcggaga aagtagatcg atccttcgag
     2701 cgtgacagtg atccagccga acgtccgccc acgccgatgg acctcggacg ggatttgcgg
     2761 aatccacgtg ccgtcgcgca gcgccacgag cggcgaacgc acccgcgcct cgcggaacgc
     2821 gtcgagaata tcgcgtcgat aggccatcgc ttcagccctc aacgcctgcg cctcgggatg
     2881 gtgaatgtcc gccaaggcgc ccgcgacggc gttcaagccc caccaactga atccgttgtt
     2941 ggacatccaa cagcgccaat cgccgatgtc ttcgagcgat ccgggcggca agaggccgcg
     3001 ctcgatgcgt ctgaggccgt cgaggttccg ggtccgggcg cgctgactgg tgatccagtg
     3061 gcaggccttg acgagtttgt ccgcgtgccg ggcgagccaa ggcttgtcgc cggtgaacca
     3121 gtagtgctcg gccatcgccc acatgatcca tccatgatgc tggttgtatc cgccctgctc
     3181 ccagccgaat gcgccgttga agactccttc ctgcgaggtg taatcgcccg gcaagcacac
     3241 cgtcccctgg tagtccagga aggtgtccag gcagcgttcg gcttccccgt gcagcccgcg
     3301 gcggtccagg tccgtgatca tcatgcacga ttcgttcccg aacacgccgt aggagaaacc
     3361 gccgacccgc gcagcgagcc gattcgcccc aggctcgcgc ccgcagttga tgagcagatg
     3421 cgacacgtga gcgcgatgga agttgttcag ctccgggatg ggcgtggtga tgcgggtcgc
     3481 ggccgcgacc cggctcctcc agaaccgcct ggtccgatcg aactcgcggt cgaattcgag
     3541 attgcgcaaa cgctcgattt catccggcgc gttcagtgta atgaacggaa tcttgaccac
     3601 aatgaagcca tcggtgccgg gcggcatgtt cccgaagtac tggatcgttc catcgacctc
     3661 aggcggatag acctgaccct gcccttcaat caatgcccgg atcacttcgc cgtcagcgag
     3721 cttcgcccga accaaaccct cttcgaggtg aagcggcacg cggcgtccgt ctgcgcgcaa
     3781 cgacagcgca acgcgggcgg gcttgtactc ctgggccagg ttgcgcccgg cgatcttgac
     3841 catgagaatc gtcgtgtcgt cggcttgcgt gtcagggtaa cccaggctgc ctgggtcaag
     3901 ccgggtcgca aaggcggtct ggctgtagcg gatgccctcg cattcccatt cggcatgctg
     3961 gatggggagg caatcgtcct cgatccagcg cctggtcaag cctgaccggg gaaggccgaa
     4021 gtcgtagtcg agccgctgac tgccccatct ccgtcgcaca gtgtctttgc ccggcggcgc
     4081 gtcgatccga tcgttgacgc aaaacacgga gccgctcgga ttgatgccga agcgctggcg
     4141 cccgccttcg agcccgaccg gcaggtacat gcgacctcgc ttgggcggtt gttcggccag
     4201 ggcccgttcg agcgattgct caggttcgtc gaagacccga gcatagaggg tcggcgtgcc
     4261 gttggtcctg gcccgctcgg cggctgcgcg aaaggcggaa cacccgacgc cctgacttct
     4321 cgtttgtctg agatgttcga gggcgatctg atcggccggg ccgacgactg catcgtgcat
     4381 tggcgagaga atcgtcgcgc ctttcgccac tgccgccggg tcgaagctga agtcgcctga
     4441 ggcggtacgg agcgtcacga cggtcgaatc aaacgtgttg acattcggac tgtcggcgaa
     4501 ccagacacgg cagcgcacgc catccggcaa atctcggacg ctggacctcc actgagccgg
     4561 gccggtgatt tcaacgcggc ttcccccagg gccgctaacc aggggcgcga gggcttcgac
     4621 gtagccgttg aaaacgctga gttgtccgtc ccagcatcgc tcgccagtgc cttcgccgcc
     4681 ccactcgatg ccgacctcga cggacttcca taccgagtct gtgaaggcct cgattctctc
     4741 gatcttggga gccgtgtctt cgaacaggag gcgcagccgc atcgtggtcc ggtgcctggc
     4801 cttgaagctc tcgagttggc tgaactcctt ctggtgaatc gggcggaagg tgtactccca
     4861 ggtcgtcccg gttgactcga tgtccgcgtc ggcttccaac cattggccct gatagagatc
     4921 ccccaggtgc ccccagccgg actctccggc gccggcgggt tggtctcggg gaatccgccg
     4981 ctccggccac tgggattgcc accactcgag cctggcctgg tagggcggtg gcggggtctc
     5041 cgcaaatgcc acgacgaccc gcaggatgtc tcgaggatcc tcccaccaga cgccgacgtc
     5101 gcgtccgttc tcccaggaag cacgctctcc ccagggcgcc acatccacgt ttgccccggg
     5161 ggccaggctc ggagacggat gtccgggttc cgctgcccat atcgccatgc cgaggatcat
     5221 gcaagggagt gcccagccaa aataaggaag catcgcggtc acccctgtat tagatcactc
     5281 atcgagacaa cgggcaatca atgcgcgcag tctatccttt gctcatgatt gaataactcg
     5341 cccggacgtt tcgccagacg gcgctggaaa cggggatgag ctgcgccgaa gtgacgcgcc
     5401 atccactgac ggtggggctc tcgaaggtgc gctgacccgg ttccgttgtc cctcgaggat
     5461 gcggggcgca accagtgtgg ttgccaagcg cgggagtgaa atatcaacta tttagatgcg
     5521 accccattgt ccattgtcaa tcattcgcaa cggtcatctt ctccgacttg cggcgcttct
     5581 cgaggaagcg gatgtacttg ctctgctcct cggcggtcca ggtgttcgcc tcggcgtagt
     5641 cgccgcagaa ggggaccagc aggtcgatcc agcaggctat tttctcgaat tccatgcgcg
     5701 agagcgcgac tccgtggtgg ccctcggcca gcatgtccat cagcccgctg cacgcg
//
LOCUS       DAOQRO010000235         5752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14225_length_5752_cov_5.250834, whole genome shotgun sequence.
ACCESSION   DAOQRO010000235 DAOQRO010000000
VERSION     DAOQRO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14225_length_5752_cov_5.250834"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKK20_09305"
     CDS             complement(<1..705)
                     /locus_tag="PKK20_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00122.1"
                     /translation="MKLKPLLTLGVILFVVGWACAPSYAQQHRATHLGNPNTRFAPPL
                     KTPEDLRALFQNPQLQADIGAILAQSDWQGNQEDLRSAAATADIRELRIPPGTRLPAM
                     STRKKGQPVLLKDILWAGKKPFEAYEFKFISNDRRYRVVTPKACSNFWVERLERELYP
                     GLEAIKTFTEATVCTPFETRIVVRNTGKREILRIRVADTLPPGLKLVEEQTPLAWEIA
                     SLDPSEGREYRFKVVAA"
     gene            809..2116
                     /locus_tag="PKK20_09310"
     CDS             809..2116
                     /locus_tag="PKK20_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV00123.1"
                     /translation="MTLGRILFIAWVGSAWLALVAAEPWPQFRGPRGDGTSMSAGLPI
                     EWSEQRNVRWKTPIHGRAWSSPVVWGGQVWVSTATEEGDRLSAVCLDLDSGRIIHDLK
                     LFDVEAPQFAHRFNTYASPTPVIEEGRVYITFGSPGTACLDTATGRVLWGRRDFVCNH
                     FRGAGSSPILDGDLLIMNFDGSDHQFVVALDKRTGQTAWRRERSIDFRDLDSTGKPEL
                     EGDLRKAFGTCHAAVLDGRATLLSQGAKAVYAYDPANGQELWRVEERTSHSASTRPVV
                     GLGLVFVATGWSTGQVLAIRPGKPGEVLDANADADPGTALQVLWRAKRNVPKKPSLLL
                     LDDLLYGIDDSGVATCWEALTGKAIWNERVGGNYSASVLGAERRIYCFSEEGKTTVLE
                     AGRTYRKLAENLLADGFMASPAVAGKALIVRTKTAVYRIESMP"
     gene            2256..3635
                     /locus_tag="PKK20_09315"
     CDS             2256..3635
                     /locus_tag="PKK20_09315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNV00124.1"
                     /translation="MQTSGLGDDWAPDSWQRKPVTQQPVYPDAKALDSALARLSRLPP
                     LVTSWEIESLRRQLAEATLGERFLLQGGDCSESFEDCESDSIAAKLKILLQMSLVLVQ
                     GGKKRVIRLGRFAGQYAKPRSADTETRGGVTLPVYRGDMINRANFSLADRTPDPELLL
                     RGYERSALTINFIRALIDGGFADLHHPEYWELGFVRRSPLAAEYTRMVESIGESLRFM
                     ETLTGGTLGDSSQVDFFTSHEGLHLLYEQAQTRRVPWREGWYNLATHLPWIGNRTRAL
                     DGAHIEYFRGIQNPIAIKLGGTVSSDEAIALCERLNPRNESGRLTLITRFGADRIESL
                     LPPIVDAVRRAGKVVLWSCDPMHGNTESLSSGVKTRRFDRILDELEKAFHILQEGGTH
                     LGGVHFELTGDDVTECVGGASGVTEADLARAYRSQVDPRLNYEQALEMALLLSRLMAG
                     KRSRGSTSH"
     gene            complement(3679..4062)
                     /locus_tag="PKK20_09320"
     CDS             complement(3679..4062)
                     /locus_tag="PKK20_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00125.1"
                     /translation="MSSPLALIFYESLLIGNQLMNRLQDLGYRVVVVSDLTMLTGQAA
                     QSRPLVFVAELGAMTERVCAAVHSLRAAPETRHVPILGLLKTTDTKEGEALTLAAQQA
                     GFTLLAREHGFLAQLPQLLEQVLEI"
     gene            4092..4733
                     /locus_tag="PKK20_09325"
     CDS             4092..4733
                     /locus_tag="PKK20_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV00126.1"
                     /translation="MQRLSLEPPRESPSDEAQLLERCRLGDSLAWEEVFERHYPAVGR
                     FIFQLSPGFSLEDVEEVCQETFLSAIRKLGSFRGGSSLQTWLFRIAANKGRDYRERQM
                     AAKRGGGRASVSLDAEDPEHGSAPPIPSSDPQPDAALERTEQMQMLERCLELVGDPCR
                     EVIELRYFGDLSYEEISRTLGLNVKTVSSRLSKCLDRLERLAQNLESRPRLDR"
     gene            4773..>5752
                     /locus_tag="PKK20_09330"
     CDS             4773..>5752
                     /locus_tag="PKK20_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00127.1"
                     /translation="MAQEPKAAIHDRLTAYATERRRRLGPPPTLPPHTRRVLLDAVSR
                     QYRPLSAQARPQHPSWIGGWMRWAWTGAALVAVIAGALWMARNPRLESPLEAAREGEP
                     TDNRRVLATAPPPPMAAPPTPSELPLAGTPDAPAASVALARTPSTAPSQSERGVAFKS
                     GDPETSGTAEALPILRRFRIEGDGQTIRIVDADGSIYEGTLLAAAKPIAENLDVGLAF
                     RGGRTAQEGSSPPGARAARNGFRAGQPKMGAPAPHGPTSVGSSSTPKNLAFEVRGTNR
                     SLNQSVLFSGQFVLTNRSIAIPTQVERHADGLLDPFKDLVPQSAVRGQAVL"
BASE COUNT         1057 a         1786 c         1884 g         1025 t
ORIGIN      
        1 cgccgccacg actttgaacc gatattccct gccctccgac ggatcgaggc tggcgatctc
       61 ccaggccagc ggagtctgtt cctcgaccag tttcagaccg ggcgggagcg tgtcggccac
      121 gcgaattcgg agaatctcgc gcttgccggt gttgcgcacg acgatgcggg tctcgaacgg
      181 ggtgcagacg gtggcctcgg tgaaggtctt gatcgcctcg aggcccggat acaattcgcg
      241 ttcgaggcgc tcgacccaga aattgctgca cgccttggga gtgacaacgc ggtagcggcg
      301 atcgttcgag atgaacttga actcgtaggc ctcgaacggc ttcttcccgg cccagagaat
      361 gtccttgaga agcacgggct ggcccttctt gcgcgtggac atggcgggga gccgagtgcc
      421 gggagggatg cgcagttcgc ggatgtcggc tgtggcggcg gcgctgcgaa ggtcctcctg
      481 attcccctgc cagtccgatt gggctagaat cgcgccgatg tcggcctgca actgaggatt
      541 ctgaaacaag gcgcggaggt cctcgggcgt cttcagcggc ggggcgaatc gcgtattggg
      601 gttgcccagg tgcgtggccc ggtgctgctg cgcatagctg ggagcgcaag cccacccgac
      661 gacgaaaagg atcacgccca gtgtcagaag cggcttgagt ttcatggcag ccgccaagcc
      721 taggtgagac tgcgcctgaa cacaagacac aagaccctgg gacaaattcg ctgggccgat
      781 cggacgtctg ttggtacggt cggcccgcat gactcttggt cgcattctgt tcatcgcttg
      841 ggttggatcc gcctggctgg ctttggtcgc cgccgagcct tggccccagt tccgggggcc
      901 gcgcggcgac ggaacctcga tgtccgccgg attgccgatc gagtggagcg aacaacgaaa
      961 cgtccgatgg aagacgccga ttcacggacg cgcgtggtca tcgccagtcg tgtggggcgg
     1021 gcaagtctgg gtgagcacgg cgaccgaaga gggcgatcgg ttgtctgcgg tctgtctcga
     1081 tctcgattcc ggacggatca tccacgactt gaagctgttc gatgtcgagg ccccgcaatt
     1141 cgcgcaccgg ttcaacacat acgcctcgcc gacgcccgta atcgaggagg ggcgggtgta
     1201 catcacgttc ggttcgccgg gcaccgcgtg cctggacacc gcgaccggtc gagtgctgtg
     1261 ggggcgccgt gattttgtgt gcaatcattt ccggggcgcc ggatcctcgc ccattctcga
     1321 tggcgacctc ttgatcatga acttcgacgg cagcgatcac cagttcgtcg tcgcgttgga
     1381 caagcggacc ggccagaccg catggcgacg cgagcgctcg attgactttc gggatcttga
     1441 ctcgacgggc aaacccgaac tcgaaggcga cctgcgcaag gctttcggca catgccacgc
     1501 ggcggttctc gacggacgcg cgactctgct cagccagggc gcgaaggccg tgtatgcgta
     1561 tgatccggca aacgggcagg agctgtggcg cgtcgaggag cggacaagtc actccgccag
     1621 cacgcggccc gtggtgggcc tcgggctggt ttttgtcgcc accggttggt cgaccggaca
     1681 ggtgctggcg atccgccccg gcaagccggg cgaggtcctc gatgcgaatg cggacgccga
     1741 ccccgggacg gctttgcagg tgctttggag agccaagaga aacgtgccca agaagccatc
     1801 cctcctgctg ctcgacgacc tgctctatgg gatcgacgac agcggtgtcg ccacctgttg
     1861 ggaagcgctg accggaaagg ccatttggaa cgagcgcgtg ggcggcaatt actccgcgtc
     1921 cgtgttgggc gccgagaggc gcatctactg cttcagcgag gagggcaaga ccacggtgct
     1981 cgaagcggga cgcacgtacc ggaaactcgc ggagaacctc ctggccgacg gtttcatggc
     2041 gtcgcccgcc gtggcgggca aggccctcat cgtccgaacc aagacggcgg tgtaccggat
     2101 cgagtcgatg ccatgatggc tagcgcgcat ttctcaccaa cttctgaaga acgcggagcc
     2161 gatgtcatcg gaaggcggcg gcggctttac attcgccccg ggaataggga tactcgatac
     2221 tccagtgatt tgtggaagaa cgattgacaa cagacatgca gacctcgggg ctgggagacg
     2281 attgggcgcc cgattcgtgg caacgcaagc cggtgaccca gcaacccgtt tatccggatg
     2341 cgaaggcgct tgattcggcc ctggcgcggc tctcccggct gccgccgctg gtgaccagtt
     2401 gggagatcga gagcctgcgc cgtcagttgg ctgaggcgac cctgggcgag cggtttctgc
     2461 tccagggagg cgattgctcc gagagcttcg aggactgcga atcggactcg attgcggcga
     2521 aactcaagat tctgctgcag atgagcctgg tcctggtgca agggggcaag aagcgcgtga
     2581 tccggctggg gcggtttgcg ggccaatacg ccaagccgcg gtcggctgac actgagactc
     2641 gaggcggggt gacgctgccg gtctaccggg gcgacatgat caatcgggcg aatttcagcc
     2701 ttgcggaccg cacgccggat ccggaattgc tgctgcgagg ctatgagcgc tcggccttga
     2761 ccatcaattt catccgcgcc ctgatcgacg gcgggttcgc ggacttgcac caccccgagt
     2821 attgggaatt gggatttgtg cgccgatcac ccctggccgc cgagtacacc cggatggttg
     2881 aatccatcgg cgaatcgttg cggttcatgg aaaccctcac tggagggacc ctgggcgaca
     2941 gcagccaggt cgattttttt acgagccacg agggcctgca tctgctctat gaacaggccc
     3001 agacgcgtcg ggtcccgtgg cgcgagggat ggtacaacct ggccacgcac ttgccctgga
     3061 tcggcaaccg gacccgggcg ttggacgggg cgcacatcga gtattttcgc ggcattcaga
     3121 acccgatcgc catcaagctg ggcggcacag tctcaagcga cgaggcgatc gccctctgcg
     3181 agcggcttaa ccccaggaac gaatcggggc ggctcaccct gatcacgcga ttcggtgcgg
     3241 accgaatcga gtcgctgttg ccgccgattg tggatgcggt gcggcgggcg ggcaaggttg
     3301 tgctctggag ctgcgatccg atgcacggga acaccgagtc gctcagcagc ggagtcaaga
     3361 cccgccgatt cgatcggatc ctcgacgagc tggaaaaggc ctttcacatc ctgcaagaag
     3421 gcggcactca cctcggtgga gtccattttg aattgacggg ggacgacgtg accgagtgcg
     3481 tcggaggggc gagtggtgtc acggaggccg acctggcgcg tgcctatcgc agccaggttg
     3541 atcctcgctt gaattacgag caagccctcg agatggctct gcttttgtcc cgattgatgg
     3601 ccggaaaacg ctcgcgcggc tcgacgtcgc attgaacccc tcacccgccc ccccagtcgc
     3661 gggggcccgc tcttcccttc agatttccag aacctgttcg agcagttgtg gcaattgggc
     3721 tagaaagccg tgttcgcgcg cgaggagggt gaagccggcc tgctgcgcgg ccagagtcaa
     3781 agcctcgccc tccttcgtgt cagttgtctt gagaaggccc aggatcggaa cgtgacgggt
     3841 ctccggagcg gctcggagac tgtgcacggc ggcgcagacg cgctcggtca tggcgcccaa
     3901 ttcggccacg aagaccagcg gcctgctctg ggcagcctgc ccggtcagca tggtgagatc
     3961 cgagacgacc acgacccgat agcccaggtc ctgcaaccgg ttcatcaatt ggttgccgat
     4021 cagaaggctt tcataaaaga tgagcgccaa cggcgacgac atgtcaaaac ggtgaagcac
     4081 tccggggtca attgcaaagg ctttccttag aaccacccag agagagtcca tccgacgagg
     4141 cgcagctcct ggagcgttgt cggctgggcg actcgctcgc atgggaggaa gtgttcgagc
     4201 gacactatcc tgccgtcggt cgcttcatat tccaactcag tcccggtttc tccctcgagg
     4261 atgtcgagga agtctgccag gagaccttct tgtcggcgat tcgaaagctc ggctcttttc
     4321 gcggcggaag ctcattgcaa acctggctct tccgaatcgc agccaacaaa ggccgcgatt
     4381 atcgggaacg tcagatggcc gccaagcggg gcggcgggcg cgcgtctgtc tcgcttgatg
     4441 ccgaggatcc cgagcatggc tcggctccgc ccattcccag ctccgatcca caacccgacg
     4501 cggccctcga gcgcaccgaa cagatgcaga tgctcgaacg ctgcctcgaa ctggtggggg
     4561 acccatgccg ggaggtcatc gagttgcgct atttcggcga tctgagctat gaagaaatct
     4621 cgcgaacact cggactgaac gtcaagacag tgagctcccg actcagcaag tgcctcgatc
     4681 ggctcgaacg gctggcccag aacctcgaaa gccgcccgcg actcgatcga taggaggact
     4741 cacccctttt tgccgtccaa tgaactgacg acatggccca ggaacccaaa gcagcgatcc
     4801 acgaccggct gacggcctac gccaccgagc gtcgaaggcg attgggcccg ccgcccacgc
     4861 tgccgccgca cacgcgacgc gtccttctgg atgcggttag ccgtcagtat aggccattgt
     4921 cggcgcaggc gcgtccgcag cacccgagtt ggatcggggg gtggatgcgg tgggcatgga
     4981 cgggcgcggc tctggtcgcc gtgatcgccg gcgcgctctg gatggccaga aacccgcgtc
     5041 tcgaatcgcc actcgaagcg gcccgcgaag gcgagcccac cgacaacagg cgagtcctcg
     5101 cgacggcccc gccgccaccc atggccgccc ctcccacccc atcggaattg cccctcgccg
     5161 gaacacccga tgcgccggcg gcctccgttg ccttggcgcg cacgccaagc accgccccgt
     5221 cacagtccga aaggggcgtg gcgttcaagt cgggcgatcc ggaaacctcc gggactgccg
     5281 aggctttgcc catcttgcgc cgattccgga tcgaaggcga cggacagaca atccgcatcg
     5341 tcgacgccga tggctcgatt tacgagggga ccctcctggc tgcggccaaa ccgatcgctg
     5401 agaacctcga tgtcgggctt gcgtttcggg gcggccggac tgcacaggag ggatcatccc
     5461 cgccgggcgc gagggctgca cgcaacggct tcagagcagg acagccaaaa atgggagcgc
     5521 ccgcccccca cggaccgaca tctgtgggat cgagttcaac cccgaagaat ctcgcatttg
     5581 aagtcagggg caccaatcgc tcgctcaacc agtccgttct cttcagcggt cagttcgtct
     5641 tgaccaaccg ctcgatcgcg attcccaccc aggtcgagcg gcatgccgat ggcctccttg
     5701 acccgttcaa ggacctggtc cctcagtcgg cggtccgcgg ccaggcggtt ct
//
LOCUS       DAOQRO010000236         5738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14262_length_5738_cov_5.466127, whole genome shotgun sequence.
ACCESSION   DAOQRO010000236 DAOQRO010000000
VERSION     DAOQRO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14262_length_5738_cov_5.466127"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="PKK20_09335"
     CDS             complement(<1..1498)
                     /locus_tag="PKK20_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV00128.1"
                     /translation="MNDQLQALRVRDPERTQVRSGTIRGGLMLLLAVAALSWESPGAL
                     AQENAPEARLITLDACIRTALLRNRALQIQRLNPEIARDTLSGSRGYYDPVFIGDARR
                     ESSSDSGGFDPADYSRDAIYDAESETVRLGLAGVLPTGMSYAFSGSYANSYGYRNFLD
                     FDSYNLYAGVAIQQPLLKNFWIDQGRMTIRVNKKNLEITELGVAYLTMDVINQVQQAY
                     CELLAARDHLAVQERLVAMRSNFQAGLERQVEVGMLTPPDTHLARSQHATAQAEAATA
                     RNLVTLSENALRTLLGDAWTDSADVPLAPRERLTAVTALFDLSASWDHGLRQRPDLAQ
                     LRTDLERADIDLKYRRNQLFPSLDLVAGYGRRGASTIESSNLGARIAASSSDAFGQIS
                     GGDNPSDMIGVIFTMPLMRQTERANYRVGKHLKAQAELLVKQKEELVMREISDALNSA
                     RSGLERLRAAGRAVEFARTALTAEERKLAGGKSTLFFVLQLQRDLATAE"
     gene            complement(1495..2532)
                     /locus_tag="PKK20_09340"
     CDS             complement(1495..2532)
                     /locus_tag="PKK20_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HNV00129.1"
                     /translation="MTICPPAALCVLGLWLGLACTAPTHAAGLGSVRTYYGLCDASAA
                     AILDADHFAVGDDEDNIIRVYHRRAGVLPVFSLDLSPFLEADPENPEADLEGAARLGD
                     WVYWITSHGRNRKGEDSPGRQRFFAVAASVRDGLARLDPVGMPYKRLLDDLLADPRLE
                     RFDLAAAAKLPPKTRGALNIEGLTATPEGHLWIGFRSPVPEGQALLVPMLNPAEVIAG
                     HRARLGDPILLPLDGRGIRSITLWRGGYLIVGGSTGDDAPSELYLWSGGADRPQRLAG
                     AGFTGLNPEALADFALPGEDDLLVMSDDGNLLVGKKPCKKLKKPSQKRFRVGVLSPIA
                     ISNVPDRPAIR"
     gene            complement(2529..3635)
                     /locus_tag="PKK20_09345"
     CDS             complement(2529..3635)
                     /locus_tag="PKK20_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00130.1"
                     /translation="MDPLPPIPTSPRLLWREFRMRVVPWLFLSGLVAAIAVMWTRVNI
                     GQSIVGVGEGERALITSPQPGLLRELKVRPYQLVNEGEPIAVVHPADPRVPLDLLRLE
                     LDLQRLRLQPTLPEQNAMSYERVRSDLLRIKSELAVAKVNLERAQNEVDRNEPLYEEK
                     LVSEDIYDLSLKTRQAFEAEVAEKTAAVREMEQRIEELRSLGEPEATDAGDQFRQLLS
                     RLDAAQSLAASNWGPITLVAPISGMVSAVQRQQGEYILDGEPLVVVHSLWSDRVIGYL
                     RQPYPIDPEIGLPVKVTTRTHAREQFWTSISQVGAQVDIITNALAYVRQGALVDVGLP
                     IVIDLPSQSRIRPGEIVDLWIKPADARPQDRGGP"
     gene            complement(3638..4336)
                     /locus_tag="PKK20_09350"
     CDS             complement(3638..4336)
                     /locus_tag="PKK20_09350"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNV00131.1"
                     /translation="MNSSSLDWFFQGDAAMVPTNWRAMVLGLLIALACGHVVSWVYML
                     THTGLSYSRSFVNALLLLPPIVCLVMMVLANNLVTAFGMMAVFAIVRFRNILRDTLDT
                     TYILISLVIGMAVGTQRFASAILGCVIICLVMIYLWQTSYGSRHHYDLILNLHWTRSL
                     QEMPELHRLLKRHSWKWLLASQRTHEGYEGTDLSFRLLLRNTDRLRDLLAEIGDLPGV
                     SRVTTMPAEDQSEL"
     gene            complement(4431..5249)
                     /locus_tag="PKK20_09355"
     CDS             complement(4431..5249)
                     /locus_tag="PKK20_09355"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNV00132.1"
                     /translation="MSKRIQSARYERKFVVSEALAREIREYARGLLELDEHGIGHPDG
                     AYPVHSIYLDSDAMKTYWDTLNGVKDRFKLRARYYSGDADAPVYLEVKRRANRRIVKQ
                     RAAVRREAAVGILDGQDAEPEALVSSDRQALVAFEDFNRLVRELRARPKVHVAYLREA
                     YMPASDDNSTRLTLDRLVCCAREETGRISTVLGDALPVWRGAVVLELKFTDVLADWCI
                     NLIRTFDLRQCGAAKYFQSVSLMGGRMSQHQRLPIPNRDCQWPGGQEDSVSRRG"
BASE COUNT         1028 a         1784 c         1907 g         1019 t
ORIGIN      
        1 actcggcggt cgccaaatcg cgctgaagtt gcaggacgaa gaagagggtg ctcttgcccc
       61 ccgccagctt gcgttcctcg gctgtgaggg ctgtcctggc aaattcgacc gcgcggccgg
      121 cggcccggag gcgttcgaga cccgagcggg cgctgttcaa ggcgtcggaa atctcgcgca
      181 tgacgagttc ctccttctgc ttgaccagca gttcggcctg ggccttgaga tgtttgccga
      241 cgcggtagtt ggcccgttcg gtctggcgca tcaggggcat cgtgaagatg acgccgatca
      301 tatcgctggg gttgtccccg ccgctgatct ggccgaaggc gtcgctcgat gacgctgcga
      361 tgcgcgcccc gaggttcgag gactcgatgg tgctggcgcc gcgccgtccg tagcccgcca
      421 ccaggtccag cgagggaaac agttggttgc gtcgatactt gaggtcgatg tccgcccgtt
      481 cgagatccgt gcgcaactgg gcgaggtcgg ggcgctggcg cagtccatga tcccagcttg
      541 cggagaggtc gaagagggcg gtgacggcgg tcaaccgttc ccgaggagcc agcgggacgt
      601 ccgccgagtc ggtccaggcg tccccgagca gggtcctcag cgcgttttcg gagagtgtta
      661 ccaggttgcg ggcggtcgcg gcctcggctt gggccgtggc gtgctgggat cgggccaggt
      721 gcgtgtcggg cggtgtcaac atgccgacct cgacctgtcg ttctaggccg gcctggaagt
      781 tcgacctcat ggcgaccagg cgctcctgaa cggcgaggtg atcgcgcgcc gcgagcaact
      841 cgcagtaggc ctgctgcacc tggttgatca cgtccatcgt caggtacgcc acgcccagtt
      901 ccgtgatctc gaggttcttc ttgttcaccc gaatggtcat gcgcccctgg tcgatccaga
      961 agttcttcag gagcggttgc tggatggcga cgcccgcgta gagattgtac gagtcgaaat
     1021 cgaggaagtt ccggtagccg tagctgttgg catagctgcc gctgaacgcg taactcatcc
     1081 ccgtgggcaa gacccccgca agccccagcc gaaccgtctc cgattccgcg tcgtaaatcg
     1141 cgtcccggct gtagtcggcc ggatcgaatc cgccgctatc cgacgaactc tcgcggcgcg
     1201 cgtccccgat gaaaacgggg tcatagtagc cgcgggaacc gctgagtgtg tcgcgtgcga
     1261 tctccggatt gagccgctga atctgcaggg cgcgattgcg caggagcgct gtgcgaatgc
     1321 acgcatcgag ggtgatgaga cgcgcctccg gcgcattttc ctgggccagc gcgccaggcg
     1381 attcccagct caaagcggcc acagccagca acagcatcag tccgccgcgg attgttccgg
     1441 agcgcacctg ggtccgctcc gggtcacgca ctcgaagagc ctggagttgg tcgttcatcg
     1501 gattgcgggc cggtccggga cgttcgagat cgcgatcggg gagagcacac cgacgcggaa
     1561 ccttttctgg cttggtttct tgagcttctt gcagggcttc ttgcccacga ggagattgcc
     1621 gtcgtcgctc atgaccagca aatcatcttc gcccggcagg gcgaaatcgg cgagcgcttc
     1681 tggattgagg ccggtgaaac cggccccggc caggcgttgc ggacggtcgg cgccgccgct
     1741 ccagaggtag agctcggacg gggcgtcatc gcctgtcgaa ccgccgacga tcaggtagcc
     1801 cccgcgccag agggtgatgc tgcgaatgcc tcgaccgtcg aggggcagga gaatgggatc
     1861 gcccaatcgg gcgcgatgac cggcgatgac ctcggccgga ttgagcatcg gaacgagcaa
     1921 ggcctggcct tccggaacgg ggctgcggaa gccgatccaa agatgccctt ccggtgtggc
     1981 ggtgaggccc tcgatgttca acgcgccgcg ggtcttgggc ggcagcttcg cggcggctgc
     2041 caggtcgaag cgctcgagcc gcggatcggc aagcaagtcg tcgagcaggc gtttgtaggg
     2101 cattccgacg ggatcgagcc gcgccaggcc gtcacggacg gatgcggcga cggcgaagaa
     2161 gcgctgccga ccggggctgt cctcgccctt gcgattgcgt ccgtgggaag tgatccagta
     2221 gacccagtcg ccgagccggg ctgcgccctc gaggtccgcc tcgggattct ccggatccgc
     2281 ttcgaggaac ggggacaaat cgagggagaa cacaggcagg acgccggcgc gccggtgata
     2341 gacgcggatg atgttgtcct cgtcgtcgcc caccgcgaag tgatctgcat cgaggatcgc
     2401 cgccgccgag gcgtcgcaaa ggccgtagta ggtgcggacc gacccgaggc cggccgcgtg
     2461 agtcggggcc gtgcatgcga gtccgagcca aaggccaagg acacagaggg cggcgggcgg
     2521 gcaaatggtc acgggccacc ccggtcttga ggccgcgcgt cggcgggctt aatccagagg
     2581 tcgacgattt cgcccggtcg aatgcgactt tgggagggca aatcgatgac gataggcagg
     2641 ccgacatcga cgagggcgcc ctggcgcaca taggcgagcg cgttggtgat gatgtccact
     2701 tgggcgccga cctggctgat cgaggtccag aactgctctc gcgcgtgggt gcgggtggtg
     2761 accttgaccg ggaggccgat ttcgggatcg atggggtagg gctggcgcag gtacccgatc
     2821 acgcgatcgg accagagcga atgcacgacg accagcggct cgccgtcgag gatgtactcg
     2881 ccctgctgac gctggacggc cgagaccatg ccggagatgg gtgcgaccag cgtgatgggc
     2941 ccccagttgc tggcggcgag actttgggcg gcgtcgaggc ggctcaggag ctgccggaac
     3001 tggtcccccg cgtcggtcgc ttccggctcg cccagagagc ggagttcttc gatgcgctgt
     3061 tccatttcac ggacggcggc ggtcttttcg gcgacctcgg cctcgaacgc ctggcgggtc
     3121 ttgaggctca ggtcatagat gtcctcggac accagcttct cctcgtagag cggctcgttg
     3181 cggtcgactt cgttttgggc ccgttcgagg ttgaccttgg cgacggccaa ctcggacttg
     3241 atccgcagca aatccgagcg cacccgctcg tagctcatcg cattctgctc cggcagcgtg
     3301 ggctgcagcc gcagccgctg gagatcgagt tccaaacgga gcaaatcgag cggtacgcgg
     3361 ggatcggccg gatggacgac ggcgatgggc tcgccctcgt tgacgagctg gtagggacgg
     3421 actttcagct cgcgcagcaa tcccggctgc gggctcgtga tcagggcgcg ctcaccctcg
     3481 cccacgccaa cgatgctctg tccaatgttg acccgagtcc acatcacagc gatcgccgcc
     3541 acgagtccgg agaggaacag ccacggcacg acgcgcatgc gaaactcgcg ccagaggagg
     3601 cggggggatg tagggatggg aggcagtggg tccatgctca aagctcggat tgatcttcgg
     3661 cgggcatcgt ggtcaccctc gagacgcctg gcaaatcgcc aatctcggcc aggagatcgc
     3721 gcaaacgatc ggtgttgcgc agcaacaagc ggaacgagag atccgtccct tcgtagccct
     3781 cgtgcgtccg ctggctggcc agaagccact tccagctatg gcgcttgagg agtcgatgca
     3841 gttccggcat ctcctgcagg gatcgagtcc agtgcagatt caggatcagg tcgtagtggt
     3901 gccggcttcc gtaggatgtc tgccagaggt agatcatgac caggcagatg atgacgcaac
     3961 ccaggatcgc gctggcgaac cgctgggtgc caacggccat gccgatgacc aggctgatca
     4021 gaatgtaggt ggtatcgagc gtgtcgcgca ggatgttgcg gaagcggacg atggcgaaaa
     4081 cggccatcat gccgaacgcg gtgacaaggt tgttggcgag caccatcatc acgaggcaga
     4141 caatgggcgg cagcaggagg agcgcgttga cgaaactgcg cgagtaggac aaaccggtgt
     4201 gcgtcagcat gtagacccag gacaccacgt gcccgcacgc cagggcaatc agcagaccca
     4261 acaccatggc ccgccagttg gtggggacca tggcggcgtc accctggaaa aaccagtcca
     4321 agctgcttga attcattgcc tatgactcga tccgacctct gataaacccg gaaaggccgc
     4381 ccgccagggc gcctccggcc acaccttcgc ctgccggggg ctcgtcacgc tcaccccctc
     4441 cgggacacac tgtcctcttg gcctcccggc cactgacaat cccggtttgg gattggcaga
     4501 cgctggtgct gggacattcg tccgcccatg agcgacacac tctgaaaata cttggccgcg
     4561 ccgcactgcc gcaagtcaaa cgtgcggatc aggttgatgc accagtcggc gagaacatcc
     4621 gtgaacttca gctcgagcac gaccgcgccc ctccagaccg ggagcgcatc gcccagcaca
     4681 gtcgaaatcc gcccggtctc ctcgcgcgca cagcagacca gacgatcgag cgtcagccgc
     4741 gtcgagttgt cgtcgctggc aggcatgtag gcctcgcgca ggtacgccac gtgcaccttg
     4801 ggcctggccc gcagttcccg aaccaaccgg ttgaaatcct cgaacgcgac aagggcctga
     4861 cgatctgacg aaaccaaggc ctccggttcg gcatcctggc cgtccaggat gccgaccgcc
     4921 gcctcacggc gcacagccgc ccgttgtttg acgatccgac ggttcgctcg ccgcttgact
     4981 tcgaggtaca ccggcgcgtc cgcatccccg ctgtaatagc gcgctcgcag tttgaaccga
     5041 tccttcacgc cgttgagcgt gtcccagtag gtcttcatcg cgtcggaatc gaggtaaatg
     5101 ctatgcacgg gatacgcccc atccggatgt ccgattccgt gttcgtccaa ctccaggagc
     5161 ccccgcgcat attcgcggat ctcgcgcgcc aaggcctcgg ataccacgaa cttgcgctcg
     5221 taccgcgcgc tctggatgcg tttgctcatc gggagaaccg tgccgcaatc tgcttcgctg
     5281 gaaaagcccc gaaaccccta atgtcccgcc gtcaagtcag gtgcgaagct tctctctttc
     5341 ttctgagtcc aaaacaaggt ttctcagcat ggaatgtgcc agtcaacgtc gaggcgtcac
     5401 gaacccggca attgtcgctg cactctcctt gaaccgaacc ttggaggggg gacggctcat
     5461 ccccggcaaa aggagtcggg cattgtgttc gcgggcgacg aggccctgcc tgccctgcct
     5521 gcggcgccag gttcatgttc actgggctgc tccgctagag cctgaacggg tggctggaca
     5581 aaccgacggg acgtgttatt gtttttgcac acacaaattc cccccaaacc aggagacatc
     5641 tcatgagcgc aagttgcgtg tttggcgcaa agcaatccca ttcgaagact ctctttttga
     5701 cgtggccggt cgcggccggc ctggcggcga tcggaatt
//
LOCUS       DAOQRO010000237         5735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14271_length_5735_cov_4.432042, whole genome shotgun sequence.
ACCESSION   DAOQRO010000237 DAOQRO010000000
VERSION     DAOQRO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14271_length_5735_cov_4.432042"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1607)
                     /locus_tag="PKK20_09360"
     CDS             complement(174..1607)
                     /locus_tag="PKK20_09360"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNV00133.1"
                     /translation="MTKRIVIAGALAALVLLARAQDSSDAMSAWQSLFDGRSLDGWKA
                     GEHPASFKVVDGAIACDGPRAHLFYMGGAGGARFKNFEFEAEVMTRPGANSGVYFHTA
                     FQDSGWPRAGFEVQVNNSQPAHNDYFETKKTGSLYGLRAQHKSIVSDNEWFRLQVRVC
                     GRQVQIRLNGTLVVDYVEPKAAAGRGVPRRRLGEGTFALQCHDETSKVLYRNLRVRVL
                     PEAAGGPVEEPVVDDAYRQIIALQAANFPVINAHVHLKGGLTLDEALALSRKTGVYYG
                     IAVNCGVGFGVTNDAGIHRYIDEMKGQPAFVAMQAEGREWVNMFSREAIARFDYVFTD
                     SMTYTDNQGRRMRLWMPNEVFVDDPQSFMDTLVDRAVGILSTEPIDIYVNPTFLPDVL
                     AADYDALWTEERMRRVIDAAVKHQVAIEINARYRLPSPRFIKLAKQAGAKFSFGTNNG
                     DRDVGDIAYCLQMVRECELKWQDMFFP"
     gene            complement(1737..>5735)
                     /locus_tag="PKK20_09365"
     CDS             complement(1737..>5735)
                     /locus_tag="PKK20_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNV00134.1"
                     /translation="NSGSDTASLAGWFLTDDPANLTKWCFPEIELVAGGYRVVFASGK
                     DRTNPAAKLHTNFKLSAEGGFLALVDPAGQVVSQFGAAYPRQVTDVSYGWVLGEGHAL
                     GYFTNPTPGAPNASSGPGFAPKVQFSRAGGTFTNPFSLSLSSSLSGALIRYTLDGNLP
                     TNGSTLYEGPIEVTNSLQVRARAFADGLLPGPPRSEGYVLLATNVLGFTSDLPVLVLH
                     SHGKGAPNSTRLNFAQVCVFEPKSGRTRLTDEPALSARAGVQIRGSSTEGLAKSSYKL
                     ELWDEFGMDQKHPLLGLPAESDWVLYAPNQFEPVLIHNPFIHQISRDMGRYSPRTRFV
                     EVYFNRGKGPMTSTHYAGIYVLEEKIKIGRERLAIDKLEPENLAPPSVTGGYLLKIDR
                     LDPGDVGLVAGGVTLAYVDPKEREIRLAQRRPQQNYIRDYVNAFYKALMATNWLDARL
                     GYRAYIDVEAAIDYHVLEVLSGNVDALVLSAYLHKPRNGKITFGPHWDFDRALGSTDG
                     RDSNPRLWATGPFFSAAWWNRMFTDKDFWQRWVDRWQELRESHFSLNNLHGLIDRLAA
                     EVRQAQPREFAKWRIGLRGGSYESEVRLMKNWLSNRIDFIDRQLTQPPRFSRLGGSIA
                     PGFVLQIASPPNASVYYTLDGSDPRLSTGGVSAGALSYTGPITLDRNARVIARAHDPT
                     KRQTGGPPAVSSTPWSRPVAATFVVSTPRLVITEIMFHPEPPPKGDPDLEDEFEYLEL
                     SNLGLSPIDLPGFRLTNGVEFAFTSASGVTRLAPGERVLVVKNQAAFLRRYPGTTRIA
                     GEYTGTLHNASDRLTLLGPLEEPISDFVYHDAWAPLADGFGFALVLADETTDPADLGD
                     ARRWRLSATVGGSPGAPDPAPPALPAIWIQEVLSNTSPPEQDAIEISNPTRAPVDISG
                     WYLSDDFRTPRKYRFPQGTVLPPSGLWLVTEDAFNAPGPGAFGLSATGDEVYVFSADA
                     QGRLTGYMHGFEFGPADPHMSFGLHALGGNRQAFVEQIEPTLGRPNRGPRVGPVVISE
                     IMYAPATAPSGGLGEEFVELRNLTGEPVPLFDPARPESTWRLSGAVNLVFPPGIILPP
                     HGHIVAVGFDPDRDLGRLLAFRQQYHPPDGLVLTGPWEGRLDKTSQPLRLQRPGQPRS
                     GGSSGIETPYLLVEQVHYLASAPWPVEANGTGRSLARRFDGAYADDVDNWHAVVPSPG
                     AQDTDGDGLSDDWELANGLNPYSADGQDGATGDPDRNGLVNRDAYRSGRPAWYGPTRV
                     QCAVAPNDPDSVLLEFDAVVGRRYTVFSREARSGASWRLEYTAPAILFAQRVAVQLPR
                     PDGARWYRVRVE"
BASE COUNT         1082 a         1787 c         1867 g          999 t
ORIGIN      
        1 ccaccaatcc acttgggcgt cgggccgacg cttcctgagc aaccgcagca cgggcagcgc
       61 ctgcactacg tcgcccagcg agcttggctt caggatcaga atcttcaaaa cggcaggtca
      121 gcggacagtt gccgcccagc gatgctggac acggccgcga aaagggccgg gggttacggg
      181 aagaacatgt cctgccattt gagttcgcat tcgcggacca tctgaagaca gtaagcgatg
      241 tccccgacat cgcggtcgcc gttgttcgtg ccgaaggaga atttggcgcc ggcctgcttg
      301 gcgagcttga tgaagcgcgg actcggcagg cggtagcggg cattgatctc gatggcgacc
      361 tggtgcttca cagcggcgtc gatcacccgg cgcatccgtt cttcggtcca gagggcgtcg
      421 tagtcggcgg ccagaacgtc cggcaggaat gtggggttga cgtagatgtc gatcggctcg
      481 gtgctcagaa tgcccacggc gcggtcgacg agcgtgtcca tgaaactctg cggatcgtcc
      541 acaaacactt cgttcggcat ccagagccgc atccgccgcc cctgattgtc ggtgtaggtc
      601 atcgaatcgg tgaagacata atcgaaccgg gcgatggcct cgcgggagaa catattgacc
      661 cactcgcggc cttcggcctg catggcgacg aacgcaggct ggcccttcat ctcatcgatg
      721 taccggtgga ttcctgcgtc gttggtgacg ccgaagccca cgccgcagtt cacggcaatg
      781 ccataataca cgccggtctt tcgggacagg gcgagcgctt cgtccaaggt caggccgcct
      841 ttgagatgca catgcgcgtt gatcaccggg aaattggcgg cctgcagcgc gatgatctgg
      901 cggtaggcat cgtccacgac aggctcctcg accgggccgc ctgcggcctc cggcaacaca
      961 cgcacacgga gattccgata gagcactttg ctggtttcgt cgtgacactg cagtgcgaat
     1021 gtcccttctc caagccgccg ccggggaacg ccccgacccg ccgccgcttt cggctcgacg
     1081 taatccacaa ccaacgtccc gttgagccgg atttgcacct ggcgaccgca cacgcggact
     1141 tgcagacgga accactcgtt gtcgctaaca atggacttgt gctgggcgcg gaggccatac
     1201 aggcttccgg tcttcttggt ctcgaagtag tcgttgtgcg ccggctgcga attgtttacc
     1261 tggacttcaa acccggcccg gggccagccg ctgtcctgaa acgccgtgtg aaagtacacg
     1321 cctgaattcg cccccggtcg cgtcataacc tcggcctcga actcgaagtt cttgaagcgg
     1381 gcgccgcccg cgccacccat gtagaacagg tgcgcgcgcg gtccgtcgca ggcaattgcc
     1441 ccatccacaa ccttgaacga tgccgggtgt tcgccggctt tccagccgtc aaggctgcgg
     1501 ccgtcgaaca gcgattgcca ggccgacatg gcatccgagg aatcctgggc tcgagccagg
     1561 aggaccagcg cagcgagtgc gccggcgatg acgattcttt tagtcatggt gcttggtcgt
     1621 ggttcggaac gtatgtttga cgactcatgg aggactttgg cattcaacga tccaatgcgc
     1681 gcgggaacct tgaatcgcat acgtccaact ctcaactctc aacttcatgg gagcgctcat
     1741 tcgacccgca cgcggtacca ccgggcgccg tcgggccggg gcaactggac cgcgaccctt
     1801 tgggcaaaca ggatcgccgg tgccgtgtac tcgagccgcc atgaagcccc cgagcgcgcc
     1861 tcgcgcgaga ataccgtgta gcgccggccc acgacggcat cgaattcgag gaggaccgaa
     1921 tccggatcat tcggcgccac ggcgcactgg actcgggtcg gtccgtacca ggcgggccgg
     1981 ccggaccgat aggcatcgcg attgacgagt ccattgcgat cgggatcgcc cgtggcgccg
     2041 tcctgaccgt cagcggaata gggattgaga ccgttggcca gttcccaatc gtcagacaag
     2101 ccatcgccat cggtgtcctg cgcgccgggg ctgggcacga cagcatgcca attatctaca
     2161 tcgtccgcgt aggcgccgtc aaagcgccgc gcaagcgaac gaccggttcc gttcgcctcg
     2221 acaggccaag gcgccgacgc gaggtaatgc acctgttcga ctagcagata tggcgtctca
     2281 atccccgagg acccgccgga tcgcggctgc ccgggacgct ggagacgcag cggttggctg
     2341 gtcttgtcga ggcgcccttc ccagggaccc gtcaggacca gcccgtcggg cggatgatat
     2401 tgctgccgaa aagccaggag gcgcccgaga tcgcgatcgg gatcgaagcc taccgcgacg
     2461 atgtgtccat gcggtggcag aatgatacca ggcgggaaaa ccagattcac agccccgctc
     2521 aagcgccaag tcgactcggg ccgcgccgga tcgaagagcg gtacgggttc gccggtgagg
     2581 ttgcgcagtt cgacaaactc ctcaccgagc ccgcccgacg gggctgtcgc gggggcgtac
     2641 atgatttcgc tgatcacgac ggggccgacc cgcggtccgc gattcgggcg gcccagggtg
     2701 ggctcgatct gctcgacaaa agcctggcga ttgccgccca gcgcgtgcaa tccgaagctc
     2761 atgtgcggat cggcgggtcc gaactcgaag ccgtgcatgt aaccggtgag ccgcccctgg
     2821 gcatcggccg agaacacgta gacttcatcg ccggtggcgc tcaggccgaa ggcgccgggg
     2881 ccgggcgcgt tgaacgcgtc ctcggtgacg agccacaagc cgctcggcgg caggacggtg
     2941 ccctgcggga aacgatactt gcgcggcgtc ctgaaatcgt cgctcagata ccatcccgag
     3001 atgtcgaccg gcgctcgagt ggggttcgag atctcgattg cgtcctgttc gggcggactg
     3061 gtgttactga gcacttcctg aatccaaatc gcaggcagtg ccggcggcgc cgggtcgggc
     3121 gcgccgggcg atccgcccac tgtcgcgctc aatcgccacc ggcgggcgtc acccaaatcc
     3181 gccgggtccg tcgtttcgtc ggcaagaacc agggcgaagc cgaatccgtc ggccagcggg
     3241 gcccacgcat cgtggtagac aaaatcgctg atcggctcct cgagtgggcc caggagcgtc
     3301 agccggtccg aagcgttgtg gagtgttccc gtgtattcgc cggcgatccg ggttgtgccg
     3361 gggtagcgac gcaggaaagc agcctggttc ttgaccacca gaacccgttc tcccggagcc
     3421 aatcgggtca cgccgctggc ggacgtgaag gcgaactcga cgccgttggt cagccggaag
     3481 ccgggaagat cgatcggcga caagcccagg ttcgacaact cgaggtactc aaactcgtct
     3541 tcgagatcgg ggtcgccttt gggaggcggt tcggggtgaa acatgatttc cgtaatcacc
     3601 aggcggggcg tggacaccac gaaggtggcc gcgaccgggc gggaccacgg cgtcgacgag
     3661 acagcggggg gaccgccagt ctgcctcttg gtcgggtcgt gagcgcgcgc gatcacgcgc
     3721 gcgttgcgat cgagggtgat tgggccggtg taactgaggg cgccggctga cacgccgcct
     3781 gtggagagtc tcgggtccga accgtctaga gtataataaa ccgaagcatt gggtgggctg
     3841 gcgatctgca agacgaatcc cggggcgatc gaaccgccca gccggctgaa tcgaggcggc
     3901 tgagtgagtt ggcgatcgat gaaatcgatc cgattggaga gccagttctt catcaatcgc
     3961 acttcgctct cgtaggaacc gccgcgcagc ccgatccgcc atttcgcaaa ctcgcggggc
     4021 tgggcctggc ggacctcggc ggcgagccga tcgatcaggc catggaggtt gttcaaggag
     4081 aaatgcgatt cgcgcagttc ctgccagcga tccacccaac gctgccagaa atccttgtcg
     4141 gtgaacatcc gattccacca cgccgcggag aagaacggac cagtcgccca aagacgcgga
     4201 ttggaatccc gcccgtcggt cgagcccagg gcgcgatcaa agtcccaatg cgggccgaag
     4261 gtgatcttgc cgttgcgggg tttgtgcaga taggcgctca ggacgagggc atcgacgttg
     4321 ccgctgagca cttcgaggac atggtagtcg atggcggcct cgacgtcgat gtaggcgcga
     4381 tagcccagac gcgcatcgag ccagttggtg gccatcaggg ccttgtagaa cgcgttgaca
     4441 tagtcccgaa tgtaattctg ctgcgggcga cgctgggcca gtcgaatctc ccgctccttc
     4501 ggatcgacgt atgccagggt caccccgccg gctaccagac cgacatcgcc gggatccaac
     4561 cgatcgatct tgagcaaata gccgcctgtc acggacggcg gagcgaggtt ctcaggttcc
     4621 agtttgtcaa tcgccagccg ctcccgaccg atcttgatct tctcttcgag cacatagatg
     4681 ccggcgtaat gagtggatgt catcgggcct ttgccgcggt tgaagtagac ctcgacaaag
     4741 cgcgtccgcg gtgagtaccg gcccatgtcg cggctgatct gatggatgaa tgggttgtga
     4801 atcagcaccg gctcgaactg attgggcgca tagaggaccc aatccgactc cgccggcaat
     4861 ccgagcagcg ggtgcttctg gtccattcca aactcgtccc acagttcgag cttgtaactc
     4921 gatttggcca agccctcagt gctcgaaccg cgaatctgca cgccggcgcg ggcactcagc
     4981 gccggttcat cggtcagccg ggtgcgtcca gacttcggct cgaacacgca cacttgggcg
     5041 aagttcaatc gggtcgagtt gggcgcccct ttcccatggg agtgcaacac cagcacgggg
     5101 aggtccgacg tgaagcccag gacattggtc gccaacagga cgtagccctc gctccgcggc
     5161 gggcccggca gcaggccgtc ggcaaaagcc cgagcgcgca cctggaggct gttggtgacc
     5221 tcaatcggcc cttcgtagag ggtcgagccg ttggtcggta ggttgccatc gagtgtgtaa
     5281 cggatcaggg ctccgctcag actcgacgag agcgacaacg agaacgggtt ggtgaaggtg
     5341 ccccccgcgc gcgagaactg caccttcggc gcgaaaccgg gtccgctcga ggcattcggg
     5401 gcgccaggcg tgggattggt gaagtaaccc aaggcatggc cctcacccag cacccagccg
     5461 taggaaacgt ccgtcacctg ccggggatag gccgcgccga attgcgaaac gacctggccc
     5521 gccgggtcga ccagggcgag aaagccgccc tcggcggaga gcttgaagtt cgtgtggagc
     5581 ttcgccgccg gattggtgcg gtccttgccg gatgcaaaaa ccacccgata gccaccggca
     5641 accagttcga tctccggaaa acaccacttc gtgaggttcg cagggtcgtc ggtgagaaac
     5701 cagcccgcga ggctcgccgt gtcgctgccc gagtt
//
LOCUS       DAOQRO010000238         5720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14326_length_5720_cov_5.525154, whole genome shotgun sequence.
ACCESSION   DAOQRO010000238 DAOQRO010000000
VERSION     DAOQRO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14326_length_5720_cov_5.525154"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKK20_09370"
     CDS             <1..1044
                     /locus_tag="PKK20_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00135.1"
                     /translation="RQAVAAARKYNRIVQAGTQGRSNEGLREALDWVRAGNLGKIKVA
                     RGLCYKRRASIGKVSGPQPIPEGIDYDLWCGPALKLPLMRQRLHYDWHWVWNTGNGDI
                     GNQGIHQMDVARWALGKGELSPRILSVGGRFGYIDDGETPNTQFAIHDYGDALLIFEV
                     RGLPSGAPEPTAAQETGQKVREEMDKYRGQSIGHVIECENGYLTGTTVYDNDGKEIRK
                     FNARGENDHFANFIKAMRSRNVADLKSDILEGHLSSALCHTSNISYRLGQKADPGSIK
                     QALEAHPAGLETFERFQAHLARHKIDLNLDKAALGAFLEMDPATERFRGNDAANALLT
                     RPYRAPFIVPEIV"
     gene            1108..2877
                     /locus_tag="PKK20_09375"
     CDS             1108..2877
                     /locus_tag="PKK20_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00136.1"
                     /translation="MTPRFLSACAAACSLAAVLGQGNPAAFNPGVPWPATDALGRTLP
                     LSDEVGPPQPNRWVGIFYFLWHDNRGGKSPHWDGPYDITRILERDAEALKKPDSPLWG
                     PIGMYHYWGEPLYGYYLSTDPWVLRRHAQLLADAGIDTLIFDTTNAVIYRDVFLALCE
                     VFRQVRREGGRTPQIVFMVNTKAGETAQKIHRELYEKGLYHELWFHWQGKPLMICDPN
                     DASPVLREFFTLRRAHWPFEMIDTPHAWHWEAAYPQPYGYTDDPNQPEQVNVSVAQNL
                     RRSDAKVTNMSSGDARGRSFHQDRMESAPGAVNWGYNFQEQWRRAFDLKPPFVMVTGW
                     NEWIAGRWGKLDGPLVFVDQFDQENSRDIEPAKVGHRDNYYWQMIANVRRFKGAPPLP
                     AASPPTTIDIGRGFGQWNAVRPEFRDHLGETGPRDHDGAAGLHYADRSGRNDLLDFKV
                     ARNENTVFFYLRTRDAIAPADTLDGLWLLIDADQDPRTGWEGFDCIVNRTPIRDGKLS
                     LERNAGGWNWKSEGEVPFRAEHNELHLAVPRSALGLSSGPDSLSLDFKWIDNAQRPGD
                     LIDVYISGDSAPEGRFVFRYEAR"
     gene            2921..4384
                     /locus_tag="PKK20_09380"
     CDS             2921..4384
                     /locus_tag="PKK20_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNV00137.1"
                     /translation="MSGNVMTPSAPAGKLAEFRGRPFLPVAAALLMAGAWLVWLSFGP
                     DRTLTPRVPGSDRPPGAEGPVGGNPVLNGKLVRGEGRPANLPGLWPGFRGPDHSGIDP
                     GAAGLARTWPPGGPQVWWSVDVGEGYAGPAVRSGRVYLMDYDQEKKQDALRCLSLEDG
                     KEIWRFVYPVSVKRNHGMTRTVPAVSDKFVVAMGPKCHVVCLDAVSGELKWGLDLVRD
                     FGTTVPPWYAGQCPLIDGDAVILAPGGKDALLMAVELESGAVRWRTPNPNGWKMTHSS
                     VTPITAAGHRQYVYCAHLGVVGVSTQDGSLLWETTDWKISIATVPSPVALDAGRVFLS
                     GGYEAGSLMLEISGDAGQWKVSPLFRLDSTVFGAEQHTAILYGGHLYGMRTDGRFICL
                     TLDGKVAWASDSSEPFGRGGGAYLMADGLVYAFNDTGRLTMIEANPAKYTPLGHAQVL
                     QGRESWGPMALAGTRLLVRDLTRLVCLEVGAGRGTNK"
     gene            complement(4406..4669)
                     /gene="rpsT"
                     /locus_tag="PKK20_09385"
     CDS             complement(4406..4669)
                     /gene="rpsT"
                     /locus_tag="PKK20_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNV00138.1"
                     /translation="MPNTKSAMRRVRSNERRHAHNRAVKSRLKTCEKNYLATIASGKK
                     DEALKALSMVNSALDKAAKTGVIHKATAERRTSRLSRRLSALK"
     gene            complement(4882..5211)
                     /locus_tag="PKK20_09390"
     CDS             complement(4882..5211)
                     /locus_tag="PKK20_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNV00139.1"
                     /translation="MAAVSDPPTGPWPPSEPIELPIDGVLDLHAFPPRQVKALVTDYL
                     AACQERGILQVRIIHGKGIGALRRTVHALLSRHPEVIEFGLDHAIFSGAGATVVRLRP
                     SQARTVS"
     gene            complement(5211..>5720)
                     /locus_tag="PKK20_09395"
     CDS             complement(5211..>5720)
                     /locus_tag="PKK20_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNV00140.1"
                     /translation="RAAGPHLDIILFGDDLGMQSGPQVSPRMYREFFFPRQQVLWRRT
                     KELAPHIKIMLHCCGGVRELLPGLIEAGLDAINPVQITCRGMAPGELKREFGARLTFW
                     GGGCDTRSILSGGTPDQVRANVRELVSILKPGGGFVFQQVHNILADVPPANVVAMFEA
                     AFEAGRYEP"
BASE COUNT         1038 a         1827 c         1852 g         1003 t
ORIGIN      
        1 cgccaggcgg tggctgcggc gcgcaagtac aatcgcatcg tccaggccgg cacgcagggg
       61 cggtcgaacg agggcttgcg cgaggcgctc gattgggtcc gcgccggcaa tctcggcaag
      121 atcaaagtgg cccgcggtct ctgctacaaa cgccgcgcca gcatcggcaa ggtcagcggc
      181 ccccagccga tccccgaagg catcgactac gacctgtggt gcgggccggc tttgaaactc
      241 ccactgatgc gccagcgcct gcactacgat tggcactggg tctggaacac gggcaatggc
      301 gacatcggca accagggcat ccaccagatg gacgtcgccc gctgggcgct cggcaagggc
      361 gaactctcgc cccgcatcct gagcgtcgga ggacgcttcg gctacatcga cgacggcgaa
      421 acacccaata cccagttcgc catccacgac tacggcgatg ccctgttgat cttcgaggtc
      481 cgagggctcc cctcaggcgc gccggaaccc acggcagcgc aggagaccgg ccagaaagtg
      541 cgcgaggaga tggacaaata ccggggccag agcatcgggc atgtcatcga gtgcgagaac
      601 ggatacctga ccggcacgac cgtttacgac aacgacggca aggagattcg caagttcaac
      661 gcccggggcg aaaacgatca cttcgccaat ttcatcaagg caatgcgaag ccgcaacgtg
      721 gccgacctga agagcgacat cctcgaaggc catctgtcga gcgccttgtg ccatacctcg
      781 aacatctcgt atcgcctggg ccaaaaggcg gacccgggat cgatcaagca ggccctcgaa
      841 gcgcatccgg ccggacttga gaccttcgag cgtttccagg cgcacctcgc caggcacaag
      901 atcgacctca acctcgataa ggcagccctc ggtgcgtttc tcgagatgga cccggccacg
      961 gaacggttcc gcggcaacga cgccgcaaac gccttgctga cccgccccta ccgcgctccg
     1021 ttcattgtcc ccgagattgt gtgagttccc cccaccgcgc actcgccatc ccccaatcgc
     1081 cacctgctat ggagcactca acatagcatg acaccgcgct ttctctcggc ctgtgccgcc
     1141 gcctgctcgt tggccgcggt tctcggacaa gggaatcccg cggcgttcaa cccaggagtg
     1201 ccctggccgg caacggacgc ccttgggcga acgttgccgc tttcggacga ggtaggaccg
     1261 ccccagccca atcgctgggt cggcatcttc tacttccttt ggcatgataa ccggggcggg
     1321 aagagtccgc actgggacgg cccgtacgac atcacccgga ttctcgaacg ggatgcggag
     1381 gcgttgaaga aaccggactc gcccttgtgg ggcccgatag gcatgtacca ttactggggg
     1441 gagccgctct atggctacta cttgagcacc gatccctggg tcctgcgccg ccacgcccaa
     1501 ttgctcgccg atgccggaat cgacacgctg atcttcgaca caacaaacgc cgtgatctac
     1561 cgtgatgtgt ttctcgcgtt gtgcgaggtg tttcgccagg tgcggcgcga gggcgggcgc
     1621 actccgcaga tcgtcttcat ggtcaacacc aaagcgggcg agaccgctca gaaaatccat
     1681 cgcgagcttt acgagaaagg gctctaccac gaactctggt ttcactggca gggcaagccc
     1741 ctgatgatct gcgatccgaa cgacgcgagc ccggtcttgc gggaattctt caccctgcgg
     1801 cgggcccact ggccgttcga gatgatcgac acgcctcacg cgtggcattg ggaagcggcg
     1861 tatccccagc cctacggtta caccgacgat cccaatcagc ccgaacaggt caacgtgtcg
     1921 gtggcgcaga acctgcgccg cagcgacgcc aaggtgacca atatgagttc gggcgatgcc
     1981 cggggccgca gctttcatca ggaccggatg gaatcggcgc ccggcgctgt gaattggggg
     2041 tacaatttcc aggaacaatg gcgccgcgca ttcgatctca aaccgccgtt cgtcatggtc
     2101 accggttgga acgagtggat tgcgggccgc tggggcaagt tggatgggcc gctggtgttt
     2161 gtcgaccagt tcgatcagga aaacagtcgc gacatcgagc cggccaaggt cgggcaccgc
     2221 gacaactact attggcaaat gatcgccaac gtccgccggt tcaaaggtgc gccgccgctc
     2281 cccgccgcct cgccgcccac aacaatcgac atcggtcgag gcttcggcca gtggaacgcc
     2341 gtgcggcctg agttccggga ccacctgggc gagaccggcc cgcgcgacca cgacggcgcc
     2401 gccggcttgc attacgccga tcgatcaggc cgtaacgacc tgctcgattt caaggtggct
     2461 cggaacgaaa atactgtctt cttctatctc cggacccggg atgcaatcgc acccgcggac
     2521 acgctggacg gtctgtggct cctgatcgat gccgatcagg acccgaggac gggctgggag
     2581 ggatttgact gcatcgtcaa tcgcacgccc attcgcgatg ggaagctgag cctcgagagg
     2641 aatgcaggcg gctggaactg gaaatccgaa ggcgaggtgc cgtttcgcgc cgagcacaac
     2701 gaactgcacc tggccgtgcc gcgcagcgcg ctcgggctgt catccggacc ggattcgctc
     2761 agcctcgact tcaagtggat cgacaacgct cagaggccgg gcgatctgat cgacgtctac
     2821 atcagcggcg actctgcgcc cgagggacgg ttcgtgttcc ggtacgaggc gcgctgactt
     2881 tctattgtga aggcgtcggg aaggcgggta acctgccgat gtgtctggca atgtcatgac
     2941 cccgtccgcg ccggccggca agctcgcgga attccgcggg aggccctttc tgcccgtggc
     3001 ggccgcgctt ctcatggcgg gcgcatggct ggtctggctc agtttcggtc cggaccggac
     3061 cctgacgcct cgcgtgcctg gctctgaccg acctccggga gcggagggtc cggtcggagg
     3121 caatcctgtt ctgaacggaa aactggtgcg cggggaaggc cgccccgcga atctgccggg
     3181 tttatggccg ggctttcgcg gcccggacca ctcggggatc gatccgggtg cggcgggttt
     3241 ggctcggacg tggcctcccg gcggtccgca ggtctggtgg agtgttgacg ttggcgaagg
     3301 ctatgccggg ccggcggttc ggagcggtcg tgtctatctc atggattacg accaggagaa
     3361 gaagcaggac gccctgcgct gcctctcgct cgaagacggc aaggaaatct ggcgcttcgt
     3421 ctatcccgtc tcggtcaagc gcaaccacgg catgacccgc acggttcctg ccgtgagcga
     3481 caagttcgtc gtcgcgatgg gaccgaagtg ccatgttgtc tgcctcgacg cagtctcggg
     3541 cgagttgaag tggggcctcg acctggtgcg ggacttcggc acaaccgttc cgccctggta
     3601 tgccggccaa tgtccgttga tcgatgggga cgcggtcatc ctggcccccg gcggcaagga
     3661 cgcgctgctg atggcggtcg aactcgagtc gggcgcagtg cgttggcgca cgcccaatcc
     3721 gaacggctgg aagatgacgc attcatcggt gacaccgatt acggcggcgg gccaccgcca
     3781 gtatgtctat tgcgcccacc tcggcgtggt cggtgtttcg acccaggacg gatcgctcct
     3841 ctgggagaca accgactgga agatcagcat cgcgaccgtc ccgtcaccgg tggcgcttga
     3901 cgccgggcgc gtctttctct cgggcggtta cgaggcgggg agcctgatgc tcgagatttc
     3961 tggcgacgcc gggcaatgga aggtcagccc gttgtttcgt ttggactcga ccgtattcgg
     4021 ggctgagcag cacacggcga tcctgtatgg cggccatctc tatggcatgc gcacggacgg
     4081 gcgatttatc tgcctgactc tggacggcaa ggttgcctgg gcgagcgatt cgagcgagcc
     4141 ctttggccgt ggcggaggcg cttacctgat ggcggacggc ctggtttacg cgttcaacga
     4201 caccgggcgg ctgacgatga tcgaggccaa tccggccaag tacacccctc tgggccacgc
     4261 ccaagtgttg cagggacgcg aatcgtgggg gccgatggcc ctggccggaa cacgcttgct
     4321 ggtgcgcgat cttacccgtc tggtctgcct cgaagtcggc gcggggcgcg ggacaaacaa
     4381 gtaaaccaga ctcgggacgg agggcttact tgagggcgct caaccggcgg ctcagacggg
     4441 aggtgcggcg ttccgcggtg gccttgtgaa tcacgccggt cttggccgcc ttgtcgaggg
     4501 ccgagttcac catgctgagg gccttaagag cctcgtcttt cttccccgac gcaatggtcg
     4561 cgaggtagtt cttctcgcag gtcttgagtc tcgatttgac agccctgtta tgcgcgtgcc
     4621 gacgctcgtt gctgcggacg cgccgcatgg cggatttcgt attcggcata gttgtcttcg
     4681 gtcaaaaagt gggcgggatc gtaacggaat gaccggtgtt gtcaacggct cgtttccgat
     4741 gcgcgcgcgc caaagggcca gttcgtgatg cgcagcggcg acgcctcccg gccgcatgac
     4801 aggaccccgt cgccgacggg caccttcttc actcgcgtca gcaggggacg agccgtcccc
     4861 actccggcct tctcgccgtc atcaactgac ggtgcgcgcc tgggacggtc ggaggcgcac
     4921 cacggtggcg cccgccccgc tgaaaatggc gtggtccaaa ccgaactcga tcacctcggg
     4981 atggcgcgac aacagggcat gcaccgttcg tcgcaatgcg ccaatccctt tgccgtgaat
     5041 gatccgaacc tggagaatcc cccgctcttg gcaggccgca agatagtcag tcaccagcgc
     5101 cttcacctgc cggggcggaa acgcgtgaag gtccagcact ccgtcgatcg gcaactcgat
     5161 cggctccgaa ggcggccagg ggcccgttgg cggatcagac acggctgcca tcatggttcg
     5221 tagcgccccg cctcgaatgc cgcttcgaac atcgccacga cgtttgcggg cggcacgtcg
     5281 gcaaggatgt tgtgaacctg ttgaaaaacg aaaccgccgc ccggcttgag tatcgagacc
     5341 agctcgcgga cgttggcgcg gacctgatcg ggtgtccccc cgctcaggat ggagcgcgtg
     5401 tcacaaccgc caccccagaa agtcaggcgc gcgccgaatt cgcgcttgag ctcgccgggc
     5461 gccatgccac gacaggtgat ctgcacaggg ttgatggcgt cgagccccgc ctcgatgagg
     5521 ccgggcagca gctcgcgcac tccaccgcag caatgcagca tgatcttgat gtgcggggcc
     5581 agctccttgg tccgccgcca caggacctgc tgccgcggaa aaaagaactc gcggtacatc
     5641 cggggcgaca cctggggtcc ggactgcatc ccgagatcgt cgccgaacag gatgatgtcg
     5701 agatgcgggc cggcggcgcg
//
LOCUS       DAOQRO010000239         5720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14327_length_5720_cov_4.777405, whole genome shotgun sequence.
ACCESSION   DAOQRO010000239 DAOQRO010000000
VERSION     DAOQRO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14327_length_5720_cov_4.777405"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKK20_09400"
     CDS             <1..463
                     /locus_tag="PKK20_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00141.1"
                     /translation="ILQVALSPGGQRLLVLAKDGTVAFWSVPAGEAAGQLATPITGSS
                     SAVFSPDGRHVAVLRATSGIGEARVWDVANGQPVTPALPHAGVITSASFGPDGSLLLT
                     ASFDGSARVWNLATGVPPIPPLLDLHSPIPTTKDAVGSEDFIHAFLLKQNP"
     gene            584..3643
                     /locus_tag="PKK20_09405"
     CDS             584..3643
                     /locus_tag="PKK20_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV00142.1"
                     /translation="MSIGILLLLVMAMSVSAGEGALDSERVGDLGSEACLVFEGNQTF
                     SRHSILNVLGVSLEFHARAHPSAPLRDYLAWIEQTVLRSYQHGGFAKAAVQVRADHTA
                     QCVVVHIIEGPRFRCGDVKVSGLDPTLAQQLKDRLRSAADLLEARKEAGLPGFSWPWN
                     ERQPVPADPHTRVTMEQSVRKALAELNRHQAEVRIEFELDESRLEAALLIQVQNPGVV
                     GTLDRIEVTGLSVNSREDLLAFLGLRAGAALAGDVAADAVRRLHDSGRFRTHLAKLVP
                     QAEAGRFKLELALVECASAPPLSRELTAEEQAFLKLREWLLQWENRAEDWVLEFDAKG
                     TDRHHRIELVLGAAGLAIVHREPMVADPPAFSQVLVATPEFVGFYSTSERTKLVGNRW
                     RGQVKAFGGISADPTERTDGRAGLGFELGAGWQAGSQNAPFALRLTLAPVAFVILAHP
                     QHADCRVKQGLLSVRSHPHAEEVMELTADAATGRLIRYSVTGSGMPADGIRLTLMLRS
                     EEGALARVVREVATGSASYTNVFDPQHPWSSSLGSLGRTFRGLWQKEHAELLEKLGRK
                     IPGGLTPEELLGAVVALEKLPWRELLEPFDRLSNTAPEPDEAEPFPFVLDSPLPEATA
                     GSDWIKLFGGLLLRANDTYWPRGSWPWAVLRDATFLATGRLDCVTNDITNLSQSSEIG
                     PLGCLAAAATFGRFDPHLAVPFARQGATRSTIAAFQADLELLVRGETAGQQFLRTTLQ
                     RVPAVENQHLRPLLKLLRSENLPLIQDGLSALSANPDQPPDETLRPVIERHWESDLRP
                     RLLTAFAERCLAASPEKVAARATSLMTEMAGWLQQAAAQHYPPAQMLLSQFYLNGIGV
                     REDYAAAATLLRQAAERNHPHAGCTLARLYRDGRGVPRNLPEAVRWFRKEAEAGCDHA
                     MFDLAEVLLASAGGSTEETDEAVRWLRESASHGFAQAQSRLGSLLSDGISIKPDYPEA
                     WIWYTLAAAQGDRIAGMDARRIERKLTADQLESARQRVAHLGKTLKP"
     gene            3755..5182
                     /locus_tag="PKK20_09410"
     CDS             3755..5182
                     /locus_tag="PKK20_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV00143.1"
                     /translation="MNSTTSPVSTSLPERQTPFPGARPALVFLLLINLFNYVDRYVLA
                     AVVGPIKRTFFGEGDFAGREDALSLALNWFQQHVGFKPDDACLGLLATAFMAMYMIGS
                     PAFARLAERHSRWVLMGIGVVVWSLASGASGLAATFLALLVTRCCVGIGEAAYGPVAP
                     TVLSDYYPVKQRGRVLAIFYLALPVGTALGFMLGEMVAKSGIGAWGASTLGAKPESWR
                     WAFYLVVIPGIVLGVWSFFLREPPRGQADLTAAVAARKTEWRDYLILLRTPSYVLCTV
                     GMAAMTFAMGGIGFWMPYYLEGRPGAPANPITVFGAILVLAGLSGTLLGGWLGDKLRA
                     RFGGAYFLVSALAMLSGFPVFLAVLRAPFPWIWVLIFLTLFCLFFNTGPTNTILANVT
                     HPSMRAAGFALNIFVIHAFGDAVSPTIIGLLNGYFGGRPEDMNKSFLVVGLTFLAAGG
                     LWLTGMKFLKRDTELAPSRLRVPAQ"
     gene            complement(5351..>5720)
                     /locus_tag="PKK20_09415"
     CDS             complement(5351..>5720)
                     /locus_tag="PKK20_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00144.1"
                     /translation="VQTDAPHPGGFNVLLVDEFTEHQCLQAYRRGAFFGSQFGGQALT
                     FQRIELRDGKLTIETNSKGDLTVVTDQNRTGTADADRMVYEIPTNTQKIPTIKYVRIE
                     ASNGQERIFSQPIRFVRSAK"
BASE COUNT          950 a         1836 c         1828 g         1106 t
ORIGIN      
        1 gattctgcaa gtggccctca gtcctggagg acaacgtctg ttggtgttgg ccaaggacgg
       61 gacggtggcg ttctggagcg tgcccgccgg tgaagctgcc ggccaactcg ccaccccgat
      121 caccgggagt tcctcggctg tgttcagccc agacggccgc cacgtggccg tgctgcgcgc
      181 caccagcggc atcggtgagg cccgcgtgtg ggatgtggcc aacggacaac cggtcacacc
      241 tgcgctgccc catgcgggcg tcataaccag tgcctccttc gggccggacg gcagcctgct
      301 gctcaccgcc agcttcgacg gcagcgcccg ggtgtggaac ctggcgaccg gtgtgccgcc
      361 cattccaccg ctcctggact tgcattcgcc gatccccacc acgaaagacg ccgtcggctc
      421 ggaggatttc atccatgcct ttttgctcaa gcagaacccg taaacccctg agtctatgaa
      481 cacgcacctt gcattctggg ctgacaaccg atcacgcccg tcttcaccag ctcctgaagc
      541 gaatgagttc agccgcgcgc cgacggtccg accgagctgg ggagtgagta tcggaatcct
      601 gttgctcctg gtcatggcca tgagcgtcag cgccggcgaa ggcgcgctcg attcggagcg
      661 tgtgggagat ttgggcagtg aggcctgcct ggtcttcgag ggtaaccaga cgttctcccg
      721 gcacagcatc ctgaatgtcc tgggcgtgag cctcgagttc catgcccggg cgcatccctc
      781 cgcgccgctt agggattact tggcctggat cgagcagacc gtgttgcgga gctatcaaca
      841 tggaggattt gcgaaggccg ccgtgcaggt gcgggccgac cacactgcgc agtgtgtcgt
      901 ggtgcatatc attgaaggac cacgcttccg gtgcggcgat gtcaaagtgt ccggcctgga
      961 cccgacgctg gcgcagcagt tgaaggatcg gttgcgctcc gccgccgacc ttctggaagc
     1021 ccggaaagag gctggacttc ccggcttctc gtggccgtgg aatgaaaggc agcccgtccc
     1081 ggccgacccc cacactcgag taacgatgga acagagcgtc cgaaaggccc tagccgaact
     1141 gaatcgtcat caggcggaag tccggatcga gttcgagttg gacgaaagtc gcctcgaggc
     1201 cgctctgctc atccaggtgc agaaccccgg cgtggtcggc accctggacc ggatcgaggt
     1261 gacgggtctg agtgtcaact cgcgggaaga cctgctggcc ttcctggggc ttcgcgctgg
     1321 ggcggctttg gcgggcgatg tggctgcgga tgcggtccga cgccttcatg attccggtcg
     1381 ctttcgcacg cacctggcga agctggtgcc gcaggctgaa gcggggcgat tcaagcttga
     1441 actggcgctg gtcgagtgcg ccagtgcgcc gcccctgagc cgggaattga cggctgagga
     1501 acaggccttc ttgaaactgc gcgagtggtt gctgcaatgg gagaaccgcg cggaagactg
     1561 ggtgctcgaa ttcgatgcca agggaacgga ccgccaccac cgaatcgaat tggtgctggg
     1621 agcggcgggt ctggcgatcg tgcaccgcga accgatggtc gccgaccctc cggcgttctc
     1681 ccaggtcctc gtggcgaccc cggagttcgt cggcttttac tcgacctcag agcgaacgaa
     1741 gctggtcggg aaccgctggc ggggtcaggt aaaggccttt ggggggataa gcgccgatcc
     1801 gactgagcgg acggacggcc gggccggtct tggctttgaa ctcggcgccg gttggcaagc
     1861 cggctcccag aacgcgccct ttgcattgcg cctcaccctg gcgcccgtgg ccttcgtgat
     1921 cctggcccac ccgcaacacg cagactgccg cgtcaagcaa ggtctcttgt ccgtccgcag
     1981 ccacccgcac gctgaggaag tcatggaatt gacggccgac gcggccaccg ggcggctgat
     2041 ccgatattcc gtcacgggtt caggaatgcc ggcggacgga atccgtttga cgctgatgct
     2101 gcggagcgag gaaggcgcgc tggcccgcgt ggttcgcgaa gtggcgaccg gcagcgcaag
     2161 ttacacgaac gttttcgacc cccagcatcc gtggagcagt tccctcgggt cgctcgggcg
     2221 cacattccgg ggtttgtggc aaaaggaaca tgccgaacta ctcgagaagc tcggccggaa
     2281 aatccccggc ggtctgactc cggaagaact gctgggtgca gttgtcgccc tcgaaaagtt
     2341 gccgtggcgg gagttgctcg agccgttcga tcgcctgtcg aacaccgctc ccgagcccga
     2401 cgaggccgag ccctttccgt tcgtgctcga ttcgcctctg cctgaagcga cggcgggcag
     2461 cgactggatc aagctgtttg gcggcctgct cttgcgcgcc aacgatacgt attggccgcg
     2521 cggctcctgg ccctgggccg tgctgcggga cgccaccttc ctggctaccg gaagactcga
     2581 ctgtgtgacc aacgacatca cgaacctctc tcagagcagc gagattgggc cgttgggttg
     2641 tctcgccgcc gcggcgacat tcggtcgatt cgacccgcat ctcgcggtgc cgtttgccag
     2701 acaaggcgcc acccggagca cgattgcggc cttccaggca gacctggaat tgctcgtgcg
     2761 cggagagaca gcggggcagc aattcctgcg gactaccctt cagcgtgtgc ccgccgtgga
     2821 gaatcagcac ctgcggcccc ttctgaagct cctacgctcg gagaatctgc cgctcattca
     2881 ggatggtctc tcggccttga gcgcgaatcc cgaccagccg cccgacgaga cgctgcgccc
     2941 cgtgatcgaa cgtcattggg aatcggactt gcgcccgcgg ttgctgacgg cttttgcaga
     3001 acggtgcctc gctgcctccc ccgaaaaagt agcggcccgc gccacgtcac tcatgaccga
     3061 gatggccgga tggctgcagc aagccgccgc acagcattac ccccccgcgc agatgctgct
     3121 gagccagttc tacctgaacg ggatcggcgt aagggaggac tatgccgcgg ccgcgacgct
     3181 gctccggcag gccgccgagc ggaatcaccc gcatgcgggc tgtaccctgg cccggctcta
     3241 tcgtgacgga cgaggtgtgc cgcggaacct ccccgaagcc gtccgctggt tccgaaagga
     3301 agcggaagcc ggctgcgacc atgccatgtt cgatttggcg gaggttctcc tggcctcggc
     3361 aggcggctcg actgaagaga ccgacgaggc cgtgcgttgg ctgcgggaat ccgccagcca
     3421 cggcttcgcg caggcgcaat cgaggctggg cagcctgctc agcgacggca tcagcatcaa
     3481 gcctgactac ccggaagcct ggatttggta cacgctcgca gccgcgcagg gcgaccggat
     3541 tgccggcatg gacgcacgac gcatcgagcg gaagctgacc gccgatcaac tcgagagcgc
     3601 ccggcagcgc gtcgcgcacc tcggtaaaac cttgaaacca tgagtccttg ggccaccctg
     3661 tgcgcaggcg tccatcatca agagagagcc agcgagtttc ccatcgcaca aacgaccggc
     3721 agccagacga cgcgtctcaa gtgaatacga cactatgaac tcgacaacct cccccgtgtc
     3781 gacctctctg cccgaacgcc agacgccctt ccccggcgcg cgcccggcgc tcgtcttcct
     3841 gctcctgatc aacctgttca actacgtcga tcgctacgtg ttggcggccg tcgtcgggcc
     3901 gatcaagcgg accttcttcg gagaaggcga ttttgcgggg cgcgaggacg cgctgagtct
     3961 ggcgctcaat tggttccagc aacacgtcgg tttcaaaccc gacgacgcct gcctcggtct
     4021 tttggccacg gcgttcatgg cgatgtacat gatcggctcg cccgccttcg cccggttggc
     4081 ggagcgccac tcgcgctggg tgttgatggg catcggcgtg gtcgtgtgga gtctggccag
     4141 cggcgcgtcc gggctggcgg ccaccttcct ggcgctgctc gtgacgcgct gttgcgtcgg
     4201 cattggcgag gcggcttacg gaccggtcgc cccgactgtc ctctcggact actaccccgt
     4261 gaaacagcgc ggccgtgtgc tggcgatctt ctacctggcg ttgccggtgg ggacggcgct
     4321 cggctttatg ctgggtgaga tggtggcgaa gtccggcatc ggcgcgtggg gcgcctcaac
     4381 gctcggcgcg aagcccgaaa gctggcgttg ggccttctac ttggtcgtga ttcccggcat
     4441 cgtgttaggg gtttggagtt tcttcctgcg cgagccgccc cgtgggcaag ccgacctgac
     4501 cgccgcggtc gcggccagaa agacggagtg gcgtgactac ttgattctgc tccgcacgcc
     4561 gtcttacgtg ctgtgcacgg tgggtatggc agcgatgaca ttcgcgatgg gcggcatcgg
     4621 cttctggatg ccctattatc tcgaaggccg gcccggcgcg cccgccaatc cgatcaccgt
     4681 cttcggcgcg atcctggtcc tggcgggtct gtccggcacg ctgctgggcg gctggctcgg
     4741 cgacaaactg cgggcccgct ttggcggcgc ctatttcctg gtttccgccc tcgcgatgct
     4801 gagcggcttt ccggttttcc tcgccgtgtt gcgcgcgcca ttcccgtgga tttgggtgtt
     4861 gatcttcctg accttgtttt gtctgttctt caacaccggc ccgacaaaca ccattctcgc
     4921 caatgtcacg cacccctcga tgcgcgccgc tgggttcgcg ttgaacatct tcgtcatcca
     4981 cgcgttcgga gacgccgtct cgcccacgat catcggtctg ctcaacggct atttcggcgg
     5041 ccgcccagaa gacatgaaca agtccttttt agtggtcggc ctgacgtttc tggcggccgg
     5101 gggtctttgg ctcacgggaa tgaagttcct gaaacgcgac acggagctgg ctccgagccg
     5161 cctgcgcgtc ccggcccagt agtctcccgg ttcaggcagt ggatgtcgcc ctatgcacaa
     5221 catcatcatc acccccgtta cgtccaatgc caggccgtca gcaaacgctt cctgacggag
     5281 acgtttcccc ctgcacctga aatctgaacg atctcgacac gatgatcatt tcagcaaaaa
     5341 ttcccctcga ttattttgcg gaccgcacga agcggatggg ctgactgaaa attcgttcct
     5401 ggccgttgct tgcttcgatc cggacgtact tgatggtcgg aattttttgc gtgttggtcg
     5461 ggatttcgta aaccatccga tccgcgtccg ccgtaccggt gcggttttga tccgtcacga
     5521 ccgtcagatc gcctttggag ttcgtttcga tggttagctt gccgtcgcgc aactcaatgc
     5581 gctgaaaggt gagcgcctgc ccgccgaatt gggaaccgaa gaacgccccg cgccgatagg
     5641 cttgcagaca ttggtgttcc gtgaattcat ccacgagcag cacgttgaat ccgcccggat
     5701 gcggcgcgtc ggtttgcacc
//
LOCUS       DAOQRO010000240         5691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14446_length_5691_cov_3.696238, whole genome shotgun sequence.
ACCESSION   DAOQRO010000240 DAOQRO010000000
VERSION     DAOQRO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14446_length_5691_cov_3.696238"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5691
                     /locus_tag="PKK20_09420"
     CDS             <1..>5691
                     /locus_tag="PKK20_09420"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00145.1"
                     /translation="LEAVGALEVPDNLGTPGLPNSRRVANAGPAIWDVQHAPVLPAAA
                     QPVTVSARVHDPDGVASVVLKWRNDTAAGSFTTTPMTDPDGDGVYRATFPGLLTGQIA
                     TFSIEAADAAMPPATSRFPGPAPTGMPALECLVRFGDTLPPGVFGAYRVWVSSGNVAR
                     WQARPTRSNEPVDATFTYDNYRTVYNAAVRYRGNWREGGFEDYSRAAYMIDFPKTERV
                     LGDTQIALDFISLNYDNGTKQQEKHAYWIARQVDLASIAMRYVHVSVNGSALFRYDSF
                     SPSRSLCSRWYGDDDPHVYEQLYPHEPFANYTTTGGVKKQAKYRYCMRKKVTTVPDDD
                     FSPLYRVVDALRAPTDDLYVARVSALADIRSWAGYWVINRMCGNGDHYNSSGYPHNMY
                     TYIPPYGRSRLHVNDTDGAFQTVYSIFPDSGFLPGIMFAKPEFRRVYWRLACDVARGP
                     MDPAISTTRLNDWYQVFRDNRIAADSPNKMSNWITARRSSYLQSLAPMTNLAFKVSTP
                     NITTNTTPITVIGEAPIAVTAIEVNGLPHAVKWIGETVWQIRVALSPGANALAFRALD
                     EHGGIVGNAARTVTYTGAPVSLEGKLLISEIMYHPAAPASSFVEILNLSPTETLPLGG
                     LRVDGIDATIGHGRFIAPGEYAVVARSLPGYQSSYGNAEVVVAEFNGALDNGGETLTL
                     QWTDGTNTIVLDQVTYGGHPPWPAAADGAGASLQLIDVFRDNDRIGNWTAIQPGGTLP
                     LATPGQSNNVTARLPEFPLLWINEVMPSNTSALADNAGEFDPWIELFNAGTNAIALTG
                     CHLSNDPSNLRQWAFPDGSKIGPGERLLVWADAQPQQTDGSHLHATFHLESGSGTVVL
                     AWHYLGRTIVLDAISYADVPADCSYGSYPDGAPHARRLFHNPTPALPNSPAWPPLLVT
                     INEWMAANTRTIADPADGDFDDWFELHNAGPYPAYLGTYTLTDNLDIPTKFSIPAGTV
                     IPPGGFLLLWADEETNQNAPGQDLHVNFKLAQGGEQIGLFDPDGVPVDTVSFGPQSDD
                     VSFGRFPDGADLPAYEMVEPTPRTPNSLAGANRPPVFDPLPDQTVAEGSPLVFTVRAT
                     DPDAGDTVRYSLGLGAPPEAELNEQTGEFRWVPGEADGPSRLRFLVHAADNGTPVRIG
                     NLWVDVNVTEVNEPPRISPIADQIVDEGALLALEFTASDPDLPPNRLIFTLAPGAPED
                     AILTKDGAFTWIPDESRGDTTVTITVRVSDQGSPEYTDTTSFNVLVREVPNPPVMPSL
                     PPLFVAENSLFCLRIDAFDPDTPPSPLVFALDAAPHGATIAPLTGEIRWLPGEEIGPT
                     NATFVVRATELQPPYASSTRAYYVTVTEVNQAPVLLPIADQALREGDTLAIQAAALDA
                     DLPPQTLTFSFAGGAPADMSIDTATGRIFWPVGPDQGASTNDVTVRVTDDGPPPRYAE
                     RTFRVVVQPQSHLVINEIMYRPASNATEFIEIVNNSSSATADLDGVELTADALAFAFP
                     HGRTLEPGAFTVVVRNRTAFEAVYGTEVPIAGQWTGQLDSTADTVRLTRVAPGGQTDT
                     LDAVRFSSEAPWPSAANGQGGSLQLIDAKQDNTRVANWTAIPALGSVQSRRLLEMTNL
                     WRYEQSGRNFGVAWRNPGYDDSAWPSGRALLYADNAALPAPKNTRLTLGPTTFYFRTS
                     FLYDGPTAGARLKVNTIIDDSAIVYLNGTEVLRVGFNQDDKVAFNTFARRWVDNAALE
                     GPFLIDQDALRYGTNVVAVEVHQNNANSSDVAWGMDLEVEGTADAATPGRPNSLAHAL
                     TPFPPVFLNEAVAVNAAGPVDNAGEREPWIELLNDGPLPVSLEGWYLTDSFTDLTRWP
                     VPAGFELEAGELRLLWADGDPSQTTPTDAHTSFRIDNGGVVALVRVDGNFENGYT"
BASE COUNT         1047 a         2144 c         1552 g          948 t
ORIGIN      
        1 cctcgaggcc gtgggcgccc tcgaggtgcc cgacaacctc ggcacccccg gcctgcccaa
       61 cagccgacgc gtggccaatg ccggtccggc catctgggac gttcagcacg cgcccgtgct
      121 gcccgcagcc gctcaacctg tgactgtgtc cgcccgggtc catgatcccg atggcgtggc
      181 gtcggtagtc ctcaagtggc gcaacgacac cgccgctggt tcgttcacca ccacgccaat
      241 gaccgatccg gacggcgacg gcgtctaccg tgcgaccttc cccggcctgc tcacgggaca
      301 gatcgcaacc ttctcgatcg aggccgccga tgccgcgatg ccccccgcta ccagccggtt
      361 cccgggaccg gcgccgacgg gaatgccggc cctcgagtgt ctggtgcggt tcggcgacac
      421 actcccgccc ggggtgttcg gcgcatacag ggtctgggtg tcgtccggca acgtcgcgcg
      481 gtggcaggcc cgcccgactc gcagcaacga gcctgtcgac gccaccttca cctatgacaa
      541 ttatcgcacg gtctataacg ccgccgtccg ctaccgcggc aattggcgtg agggcgggtt
      601 cgaggactac agccgcgccg cctacatgat cgactttcca aagaccgagc gcgtcctcgg
      661 ggacacgcag atcgccctgg atttcatcag cctcaactat gacaacggca ccaaacaaca
      721 ggagaaacac gcctactgga tcgcgcggca ggtcgatctc gcctcgatcg ccatgcgcta
      781 tgtgcacgtc agcgtcaacg gttccgccct gttccgctac gactccttct cgccttcgag
      841 gtcgctctgc agccgctggt acggcgacga cgatccgcac gtgtacgaac agctctatcc
      901 gcacgagccc ttcgccaatt acaccacgac cggcggcgtc aagaagcaag ccaaataccg
      961 ctactgcatg cgcaagaagg tcaccaccgt gcccgacgac gatttctcgc ctctctatcg
     1021 ggtggtcgac gccctcagag ccccaaccga cgacctctac gtcgcccgcg tgtccgccct
     1081 ggccgatatc cggagttggg cggggtactg ggtgatcaac cggatgtgcg gcaacggcga
     1141 ccactacaac tcgtccggtt acccgcacaa catgtacacg tacatccctc cctacggacg
     1201 ctccagactc cacgtcaacg acaccgacgg cgccttccaa accgtctact cgatcttccc
     1261 cgactccggc ttcctgcccg ggatcatgtt cgccaaaccc gagttccgcc gcgtctactg
     1321 gcgtctggcc tgcgacgtgg cgcggggccc catggacccg gccatcagca ccacacgcct
     1381 caacgactgg taccaggtct tccgcgacaa tcgaatcgcc gccgactcac ccaacaagat
     1441 gagcaactgg atcactgccc gcagaagcag ctacctccaa tcgttggccc ccatgacaaa
     1501 cctcgccttc aaggtttcca ccccgaacat cacgacaaac acaacaccca tcaccgtcat
     1561 cggcgaggcg ccgatcgccg tcaccgccat cgaagtcaac ggcctccccc acgccgtcaa
     1621 atggatcggc gaaaccgtct ggcaaatccg cgtcgctctc agccccggcg ccaacgccct
     1681 tgccttccgc gccctggatg aacatggtgg catcgtcgga aacgccgccc gcacggtcac
     1741 ctacaccgga gcgcccgtgt cactcgaagg taagctcctc atcagcgaaa tcatgtacca
     1801 ccccgcggcc cccgcctcga gcttcgtcga aatcctcaac ctctccccca cggaaaccct
     1861 tcccctgggc gggctgcgcg tggacggaat cgatgccacc atcggacacg gacgcttcat
     1921 cgcgccgggc gagtatgcgg ttgtggccag gagcctgccg ggctatcaat cgagctacgg
     1981 caatgctgag gtcgtcgtcg cggaattcaa tggcgcactc gacaatggcg gagagacgct
     2041 caccctgcaa tggactgatg gcacaaacac aatcgttctc gaccaggtca cctacggcgg
     2101 ccatccgccc tggcccgccg ccgccgacgg cgccggcgca tcgctgcagt tgatcgacgt
     2161 gttccgtgac aacgaccgca tcggcaattg gaccgccatc caacccggcg gcacgctgcc
     2221 cctggcaacg ccaggccagt caaacaacgt caccgcgcgc ctccccgaat tccccctgct
     2281 ctggatcaat gaagtcatgc cgtccaacac cagcgcgctg gcggacaacg ccggcgaatt
     2341 cgacccctgg atcgaactgt tcaacgccgg cacgaatgcg atcgctctca caggctgcca
     2401 cctcagcaac gatccctcga atctgcgcca atgggccttc cccgacggat cgaagatcgg
     2461 cccgggtgaa cgtctgctgg tctgggccga cgcgcagccc cagcagaccg atggctccca
     2521 tctgcatgcc accttccacc tagagagcgg cagcggcacc gtggttctgg cctggcatta
     2581 cctgggccgt accatcgtcc tcgatgccat ctcctacgcc gatgtccccg ccgactgctc
     2641 gtacggatcc tatccggacg gcgccccgca cgcccgccgg cttttccaca atcccacgcc
     2701 ggcattgccc aacagccccg cctggccgcc gctgcttgtc accatcaacg agtggatggc
     2761 cgccaacact cggacgatcg ccgatcctgc cgatggcgat ttcgacgatt ggttcgaact
     2821 ccacaacgcc ggtccctacc cggcgtacct cggcacgtac acattgaccg acaacctcga
     2881 tatcccaacg aagttctcga tccccgccgg caccgtcatc ccgcccggcg gcttcctgct
     2941 gctctgggcg gacgaggaga ccaaccagaa cgctccaggc caggacctgc acgtcaactt
     3001 caagctcgcc cagggcggcg aacagatcgg cctgttcgat ccggacggcg tccctgtcga
     3061 cacggtctcc ttcggtccac agtccgatga cgtcagcttc gggcgattcc ccgatggcgc
     3121 cgatttgccc gcatacgaaa tggtcgaacc tactccccgc accccgaaca gcctggccgg
     3181 cgccaaccgc ccccccgtct tcgatccgct gcccgatcag actgtcgccg aaggcagccc
     3241 gctggtcttc accgtcagag ccactgatcc cgatgccggc gatacggtgc ggtacagcct
     3301 cggcctgggc gctccgcccg aggccgagtt gaacgaacag accggcgagt tccgctgggt
     3361 ccccggggaa gccgatggcc cgagccgcct gcgcttcctc gttcacgccg cggacaacgg
     3421 aacaccggtc cgaatcggga atctgtgggt cgacgtgaac gtcacggaag tgaacgaacc
     3481 gcctcggatc agtcccatcg cggaccaaat cgtggatgaa ggggctttgc tcgccctcga
     3541 gttcaccgcc agtgatcccg atctgcctcc caatcgcctc atcttcactc tcgcccccgg
     3601 cgcgcccgaa gatgccatcc tcacaaagga cggcgccttt acatggattc ccgacgaatc
     3661 gcgaggcgac accaccgtaa ccatcaccgt gcgcgtctcc gaccagggct cgcccgagta
     3721 caccgacacc accagcttca acgtcctggt tcgtgaggtg cccaatccgc ccgtcatgcc
     3781 gtcccttcca ccgctgtttg tcgccgaaaa cagtctgttc tgcctccgca tcgatgcgtt
     3841 tgatccggac acgccgccca gtccgctggt cttcgcgctg gatgccgctc cgcatggggc
     3901 gacgatcgcg cctctgaccg gcgagatcag atggctgccc ggcgaggaaa tcggtcccac
     3961 caacgccacc ttcgtcgtgc gcgccaccga gctgcaaccg ccttatgcca gttccacccg
     4021 tgcctactac gtcacggtga ccgaggtgaa ccaggcccca gtcctcctcc cgatcgccga
     4081 tcaggctctg cgcgaaggcg acacgctcgc catccaggcg gcggcgctag atgccgacct
     4141 gccaccgcaa acgctcacct tttcgttcgc cggcggcgcc cccgccgaca tgtccatcga
     4201 caccgccact ggccgcattt tctggcctgt cggacctgac cagggcgcca gcaccaacga
     4261 cgtgaccgtg cgggtcaccg acgacggtcc gcctcctcgc tacgccgagc ggacgtttcg
     4321 cgttgtcgtc caaccccagt cgcacctggt catcaacgaa atcatgtacc gccccgcatc
     4381 caacgcgacc gagttcatcg agatcgtcaa taactcttcc agtgcgacgg cggacctcga
     4441 cggcgtggaa cttaccgccg atgccctcgc gtttgcgttc ccgcatggca ggacgctcga
     4501 acccggcgcc ttcaccgtcg tggtgcgtaa tcgaacagcc ttcgaagcgg tttacggcac
     4561 cgaggtcccg atcgccggcc agtggaccgg ccagctcgat tcgaccgccg acaccgtccg
     4621 cctgacacgc gtcgcgccgg gtggccagac cgacacgctc gacgcggtgc gtttttcctc
     4681 ggaagccccc tggcccagcg cggccaatgg ccagggcggg tcgctccagt tgatcgatgc
     4741 caaacaggac aacacacgcg tcgccaattg gaccgccatc cccgctctcg gatcagtcca
     4801 gtcccgccgg ctgctcgaga tgacgaacct ctggcgctac gagcagtcgg gcaggaattt
     4861 cggcgttgcc tggcgcaatc ccggctatga cgactccgcc tggccctccg gacgcgccct
     4921 gctctacgcc gataacgcgg ccttgcccgc gcccaagaat acgcggctga ccctcgggcc
     4981 gaccacgttc tactttcgca cctcgttcct gtacgacggc ccaaccgcgg gtgcgcgcct
     5041 caaggtcaac accatcatcg atgactccgc catcgtctac ctcaacggca ccgaggtgtt
     5101 gcgggtcgga ttcaaccaag acgacaaggt cgcgttcaac accttcgcca ggcgatgggt
     5161 cgataacgcc gccctcgaag gcccgtttct catcgaccag gatgcactcc gatacggcac
     5221 caacgtcgtc gcagtcgagg ttcaccagaa caacgccaac agctcggatg tcgcctgggg
     5281 aatggacctc gaagtcgaag gcacagccga cgccgccacg cctggccgcc ccaactcgct
     5341 cgcccatgct ctgaccccgt tcccgcccgt tttcctcaac gaagccgtcg ccgtgaatgc
     5401 cgctggcccg gtcgacaacg ccggcgagcg cgaaccctgg atcgagttgc tcaacgacgg
     5461 ccccctaccc gtctcactcg aaggttggta tcttaccgat tcgttcaccg atctgacgcg
     5521 atggccggtc cccgccggat tcgagctcga agcaggggag ttgcgtctgc tctgggccga
     5581 tggcgacccg tctcaaacca ccccaaccga tgcccatacc agcttccgaa tcgacaacgg
     5641 cggcgtggtc gcgctggtcc gcgtggacgg caatttcgaa aacggctata c
//
LOCUS       DAOQRO010000241         5626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14685_length_5626_cov_4.258840, whole genome shotgun sequence.
ACCESSION   DAOQRO010000241 DAOQRO010000000
VERSION     DAOQRO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14685_length_5626_cov_4.258840"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKK20_09425"
     CDS             <1..429
                     /locus_tag="PKK20_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNV00146.1"
                     /translation="KVAPEHLHPDVLRRMRKPAVDFETFLKQFHRSSEAAGKEQYLVP
                     YFISSFPGCTDKEMAVIERFLKRQNWNLQQVQDFIPLPMTGAAAMYVTGLDLDTEQPI
                     PVVRNAGDRARQQRLLRPNRPRATPRGVSSLDDYDAGVSP"
     gene            complement(419..2500)
                     /gene="fusA"
                     /locus_tag="PKK20_09430"
     CDS             complement(419..2500)
                     /gene="fusA"
                     /locus_tag="PKK20_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV00147.1"
                     /translation="MPRDIQRIRNIGVIAHIDAGKTTVTEHMLFCSGSKHRAGEVDAG
                     TTETDCDPEEQDRGITIYSACVTFDWLDAHINLIDTPGHVDFTAEVERCLRVLDGAVV
                     VFSAREGVQAQSETVWRQADRYRVPRLAFVNKMDRDGAGFERVCGEIAQRLGAQPVPL
                     QIPVGAGPVLAGDAFRGVIDLIEMQMLEFDGESDGRVVRRGDLPEDRRAEAESWRNQM
                     LEKLYGHSDELTELALLEKPIPPALIRQVIRQATVALKIQPVLCGSALRGIGVQPLLD
                     AVAWYLPSPLDLPSVEGENPTRKGHIERRPPRSSEPFCGLVFKVLPAKTGDLSWVRIY
                     SGELKANTRALNAGKDKKENVSQLWRIHATKREQQVQKAAAGDIVGLIGLRHSITGDT
                     LCDPRHPILLESIQFPQTVISMAIEPESATEREKLADALALLQRQDPSLQVGNGETGQ
                     TLINGMGELHLEVIKNRLLRDFALNVRFHTPQVSYRETVAHGVDVTGVCQRQIAGQPL
                     FAELRIRIEPDDSGAQPVTVVNLCPPEALPAPFVTAAVEELKTCGDGGGRISGYPLTK
                     LRISILGGQMSQEHSNEMAFRIAANEAFQKGLEQAGPILLEPIMKLDILTPETYLGEF
                     LGDLQQRRALIVHTEHRDGLAAIEAHAPLHELFGYASAMRSLSQGRASCSMEPLTYAP
                     APPSTLKRIMG"
     gene            complement(2649..3068)
                     /locus_tag="PKK20_09435"
     CDS             complement(2649..3068)
                     /locus_tag="PKK20_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00148.1"
                     /translation="MNSHLSDAALGFLDRLLQLRVQDPLAAEFVMRFQQFTCPAMANG
                     GEDTAFYCYNRFAALNEVGGDPSRFGSSVNDFHRACLALLSEIPGLWDFSLVVTDNPR
                     PVDFDPRQVLMASVQAGIASGPTSGFLDELLSNQNRN"
     gene            complement(3065..3637)
                     /locus_tag="PKK20_09440"
     CDS             complement(3065..3637)
                     /locus_tag="PKK20_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00149.1"
                     /translation="MKHSRPHGNELETLARLHGVHTSYLDMARQRRFASPDTLLAILR
                     AMGVPVRRATDVPDALREARQTRACRALEPVHVVWRGRPGAIGLQLPERLPARSVRTE
                     GRLEDGEVRRCESRLDRLPETGRARANGELLVRRLLPVPRWLPTGYHRLRLECADGCW
                     ATHVFRTPKPRSVQKTTGPPHRRPSRRSAV"
     gene            complement(3655..4875)
                     /locus_tag="PKK20_09445"
     CDS             complement(3655..4875)
                     /locus_tag="PKK20_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HNV00150.1"
                     /translation="MDWLKYLLLALTQIRVTDFVDIAVVMLIIYTFLVALKRTRRSSL
                     IVVGIVIFGLLYVGAWKLDLRLTTTLLRGFFAIILVALVVIFQEDLRYFFERVGLWWR
                     ERSLPLYRRQARRLPRREVEILARTLGDLARARIGALVVVRGHDVIARHVQGGEEVNG
                     RLSEPLLRSIFDPHSPGHDGAVIIEGNQVDKLGVYLPLSKNLDELPRRGTRHAAALGL
                     SERSDALCLVVSEERGTISVVRRGRIRMVADSSELATILEAFYHEIAPQPEFRLWRDL
                     YHHNYREKVLAVVLAVSLWLVVVHRSQIVHRSFEIPVQYGLLGTNLVVTMVRPDSIVA
                     SFSAPRKEFDFLGSGDIRLVLQLWDARRGRRTFRITSTELSYPAKLNLENIEPRHVTL
                     EIDEAPAPNAKTAP"
     gene            complement(4882..>5626)
                     /locus_tag="PKK20_09450"
     CDS             complement(4882..>5626)
                     /locus_tag="PKK20_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00151.1"
                     /translation="QPVFGNNSFPGNRKATLVLDQHPGDTPPQAGLAIVDPGCGATCV
                     LVAQALLQASADIPARVATALAYGILTDTLDLYRARRGDVTQTYLEVMRHADMRALAR
                     IRNPLRSRKFFSVLGRGIREAVQYRRLVVSHLGLVDAPDRVSQVAEFLLTYERAQWCF
                     VSGRYKGRLHVSLRSVHQDAQAGEVLRNVFVQRTQAGGHGPIGGGSCRIGLDAPEEKW
                     SERERVLTERLRGRLRIRSTTEPRKPFAS"
BASE COUNT         1003 a         1672 c         1961 g          990 t
ORIGIN      
        1 aaggtcgccc ccgagcacct gcatccggac gtgctgcgtc ggatgcgcaa gcccgccgtc
       61 gatttcgaga cgtttcttaa gcagtttcat cggtcgagcg aggcggcggg caaggagcag
      121 tacctggtgc cgtatttcat ttcgagcttc ccgggctgca ccgacaagga gatggccgta
      181 atcgagcgtt ttctcaagcg ccagaactgg aatctgcagc aggtgcagga tttcatccca
      241 cttcccatga cgggggcggc ggcgatgtat gtgaccggat tggacctcga cacggagcag
      301 ccgattcccg tggtgcgcaa cgccggcgat cgagcgaggc aacagcggct gctgcgtccg
      361 aatcggccgc gcgccacccc gcgtggcgtc tcgagtcttg acgactacga cgccggcgtc
      421 agcccatgat tctcttgagc gtgctggggg gggcgggggc gtaggtgagc ggttccatcg
      481 agcatgaggc gcggccctgg ctcaggctcc gcatcgcgct ggcgtagccg aacaactcgt
      541 gtaacggggc atgggcctcg atggccgcga gcccgtcacg gtgctcggtg tggacgatga
      601 gtgcgcggcg ctgctggagg tcgcccagga actcccccag gtaggtctcg ggtgtcagga
      661 tatcgagctt catgatcggc tcgagcagga tcggtccggc ctgttccagc cctttctgaa
      721 aggcctcgtt ggcggcgatg cgaaaagcca tttcgttcga atgctcctgg ctcatttggc
      781 cgcccaagat cgagattcga agcttagtga gagggtagcc ggagattcgg ccgccgccgt
      841 cgccacaggt tttgagttct tcgaccgcgg ctgtcacgaa gggggcaggc agggcctcgg
      901 gcgggcagag gttgaccacg gtcacaggct gcgcgccaga gtcgtcgggc tcgattcgga
      961 ttcgcagttc ggcgaacagc ggctggccgg cgatttggcg ctggcagacg ccggtgacat
     1021 cgacgccgtg tgcgacggtt tcgcgatagc tgacctgggg tgtatggaac cgcacgttga
     1081 gggcaaagtc gcgcagcagg cggttcttga tcacttcgag atgcaactcg cccatgccgt
     1141 tgatgagcgt ctgtcccgtc tcgccgttgc cgacctgcag gctgggatcc tgacgctgga
     1201 gcaatgcgag cgcgtcggcg agtttctcgc gttcggtggc gctctcgggc tcgatggcca
     1261 tcgagatgac ggtctgcggg aactggatgg attcgaggag aatcgggtgc cgcgggtcgc
     1321 agagggtgtc gccagtgatc gaatgccgca ggccgatcag gcccacgatg tcgccggccg
     1381 cggccttctg gacctgctgt tccctcttgg tggcgtggat gcgccacaat tgcgagacgt
     1441 tttccttctt gtccttgccc gcgttcagag cgcgtgtgtt ggccttgagt tcgcccgagt
     1501 agatgcgtac ccaggacaga tcgccagtct tggccggaag gaccttgaag accaggccgc
     1561 agaacggctc ggaggagcgg ggggggcggc gctcgatgtg gcccttccgg gttgggtttt
     1621 ctccctcgac ggacggcaag tccagcgggc tgggcaggta ccaggcgacg gcgtcgagga
     1681 gcggctgcac gccgatgcca cgcaaggccg agccgcagag cacaggctgg atttttagag
     1741 ccacggtcgc ctggcggatc acctggcgaa tcaaggccgg cgggatcggc ttttcgagaa
     1801 gagccagttc cgtcagttcg tcgctgtgcc cgtagagctt ttcgagcatt tggttgcgcc
     1861 acgactcagc ttcggcccgc cgatcctcgg ggagatcgcc ccgtcgaacc acccggccgt
     1921 ccgactcccc gtcgaattcg agcatctgca tctcgatcag gtcgatgacc ccccgaaacg
     1981 catcgccggc gaggacgggg ccggccccaa ccgggatctg gagcgggaca ggctgggcgc
     2041 ccaggcgctg cgcaatctcg ccgcagacgc gctcgaaacc ggcgccgtca cgatccatct
     2101 tgttgacaaa ggccagacgc ggcacccgat agcggtcggc ctggcgccac acggtttcgc
     2161 tctgcgcctg gacgccttcg cgggcgctga agacgaccac ggcgccatcg agcacccgca
     2221 ggcagcgttc gacttcagcc gtgaagtcca catggcccgg agtgtcgatg agattaatgt
     2281 gggcatccag ccagtcgaat gtgacgcagg ccgagtaaat ggtgatcccg cggtcctgct
     2341 cctcgggatc gcaatcggtt tccgtggtgc cggcatcgac ctcgcctgcg cgatgcttgc
     2401 tgccgctgca gaagagcatg tgctcggtga cggtggtctt gcccgcgtcg atgtgggcaa
     2461 tgaccccgat attgcggatg cgttggatgt cgcgtggcat agagttgttt cgttttggag
     2521 agggaggtgc cgatcatgcg acgctgggtt tctctggaat ccttgatcgg ttgaatatgt
     2581 tgctgacaag tcaagtttct gtgctgcgtc aaacgggtca aggattctac taagagtctg
     2641 tgcaaaaact agttccgatt ttgattgctg agcagttcat ccagaaaacc ggatgtggga
     2701 ccggacgcga tccctgcctg gacggaggcc atcaagacct ggcgcggatc gaagtccacc
     2761 ggccgcggat tgtccgtaac caccagactg aaatcccaga gaccggggat ttcagacagg
     2821 agcgcgaggc aggcgcggtg gaagtcgttc acgctcgagc cgaagcggct cggatcgccg
     2881 ccgacttcgt tgagggcggc aaagcggttg tagcagtaaa aggcggtgtc ttcgccgcca
     2941 ttggccatgg ccgggcatgt gaactgttgg aagcgcatca caaactccgc ggcgagcggg
     3001 tcctggacgc gcagttgcag cagacgatcg agaaaaccga gcgctgcgtc ggacaaatga
     3061 gagttcacac tgcggacctg cgtgacggcc tccgatgcgg cggcccagtc gttttctgaa
     3121 cagatcgagg cttcggcgtg cggaagacgt gggtcgccca gcatccgtcc gcgcattcga
     3181 gccggagacg atgataccct gtggggagcc agcggggcac aggcaggagc cggcgaacca
     3241 acagttcccc atttgcgcgc gccctgcccg tctcgggcag gcgatcgagc cgcgattcgc
     3301 atcggcgcac ctcgccgtct tcgaggcgcc cctcggtgcg gaccgatcgg gccggaaggc
     3361 gttcgggcaa ttgcagacca atggcgcctg ggcgcccgcg ccagacgacg tgcacgggtt
     3421 cgagggcgcg gcaggcacgg gtctgacggg cttcgcgcaa ggcatccgga acgtcggtgg
     3481 cgcggcggac gggcacgccc atggcgcgga gaatggccag gagcgtgtcg ggactggcga
     3541 atcggcgctg ccgtgccatg tccagatagc tcgtgtggac gccgtgaagg cgggcgagcg
     3601 tctcgagttc gttcccgtgc gggcgtgagt gcttcatcgg cttctcacgt gtggtcaggg
     3661 cgctgtcttc gcgttcgggg cgggggcctc atcgatctcg agtgtgacat gacggggttc
     3721 gatattctcg aggttcagct tggccgggta ggagagttcg gtgctggtga tgcggaaggt
     3781 gcggcgcccg cgccgcgcat cccagagttg cagcacgagg cggatatcgc cggagccaag
     3841 aaaatcgaac tccttgcgcg gggcggagaa gctggccaca atgctatcag ggcggaccat
     3901 ggtgacaacg agattggtgc cgagcagccc gtactggaca gggatctcga aggagcggtg
     3961 gacgatctgg ctgcgatgga cgaccaccag ccacaggctg acggccagaa ccactgccag
     4021 caccttctcg cggtagttat gatggtagag atcgcgccac agacggaact cgggctgggg
     4081 agcgatctcg tgatagaagg cttcgaggat ggtggccaat tcgctcgaat cggctaccat
     4141 tcggatgcgg ccgcgccgga cgacggagat ggtgccgcgt tcctcggaca cgaccaggca
     4201 gagcgcgtcg ctgcgttcgg agaggcccag ggcggcggca tggcgggtgc ctctgcgggg
     4261 gagttcgtcg agattcttcg agaggggcag atagacgccg agtttgtcga cctggttgcc
     4321 ctcgatgatg accgcaccgt cgtggcctgg cgaatgggga tcgaagatgc tccgaagcaa
     4381 aggttcgctc aatcgtccgt tgacctcctc gcccccctgc acgtgacgcg caatcacgtc
     4441 gtgcccgcgc accaccacca gcgccccgat gcgggctcgg gccagatcgc ccagggtgcg
     4501 ggcgaggatc tcgacctcgc ggcgggggag gcgccgggcc tggcgccggt agaggggcag
     4561 gctgcgctca cgccaccaca ggcccacccg ctcgaaaaag taacggagat cctcctgaaa
     4621 gatcaccacg agcgcgacga ggatgatcgc gaagaaaccc cgcagcagcg tggtcgttag
     4681 tcgcagatcg agcttccagg cgcccacata aagcaggccg aagatcacga tcccaacaac
     4741 gatgagcgag gagcggcgcg tccgtttgag agctaccagg aacgtgtaga tgatcagcat
     4801 gacgaccgcg atatcgacaa agtcggtgac gcggatctga gtcagggcga ggagcaggta
     4861 tttcagccag tccatcggtg ttcagctagc aaagggtttg cggggctcgg tcgtgctccg
     4921 gatgcgcaga cggcccctca ggcgctcggt cagcacgcgt tcgcgttcgc tccatttttc
     4981 ttcgggcgca tccagcccga tgcggcaact gcctcccccg atcggcccat ggccgccggc
     5041 ctgggtgcgc tgaacgaaga cgttgcgcaa gacctcgccc gcctgggcgt cctgatggac
     5101 gctgcgcaac gagacgtgca ggcggccctt gtagcgccct gagacaaaac accactgggc
     5161 acgttcgtac gtgagcagga actccgccac ctgggagacg cgatcgggcg cgtcgaccag
     5221 cccgagatgc gacaccacca ggcggcggta ctggacggct tcgcggatgc cgcggcccag
     5281 gacgctaaag aacttgcggg agcggagcgg attccggatg cgcgccaggg cccgcatgtc
     5341 ggcatggcgc atcacctcga ggtaggtttg cgtgacgtcg ccgcggcgcg cccggtacag
     5401 atctagcgtg tccgtgagaa tgccgtatgc cagcgcggtc gcaacgcgcg ccgggatatc
     5461 ggcgctggct tggagcaggg cctgagcgac cagaacgcaa gtggcaccac aacccgggtc
     5521 gacgatggcc agaccggcct ggggcggggt gtcgccagga tgctgatcga gcacgagggt
     5581 ggccttccga ttgcccggaa acgagttgtt gccaaagacc ggctgg
//
LOCUS       DAOQRO010000242         5584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_14854_length_5584_cov_3.945017, whole genome shotgun sequence.
ACCESSION   DAOQRO010000242 DAOQRO010000000
VERSION     DAOQRO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14854_length_5584_cov_3.945017"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..1200)
                     /locus_tag="PKK20_09455"
     CDS             complement(388..1200)
                     /locus_tag="PKK20_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV00152.1"
                     /translation="MNTQLCAARLAPRNPVLVGSAASPAQPGPKPGAARGGRLNRFAA
                     LAVVACLTLGAASAHAAFTFFQTFDGLDLTVPDGSSLGASDTRQVSVGYGSILGVQVT
                     LELSGGYIGDLYALLIHDGVSAVLLNRPGRRTDDSLGYADSGLYVTFSDAAAQGDVHN
                     YRLTLQGSHNTPLGGPLTGTWQPDGRTSSPERVLDTDPRSGFLSSFISHTPDGAWTLF
                     LADLSPVGEARLESWGLELEYVPEPGACAGLAALGLLGWGTCRRTARRTGDH"
     gene            complement(1230..3794)
                     /locus_tag="PKK20_09460"
     CDS             complement(1230..3794)
                     /locus_tag="PKK20_09460"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV00153.1"
                     /translation="MKPMHCGMPARAQSESQSARRFSMRLWMAALAGWLALPCAFGQG
                     DLLLRETFSREVSVFVGGVQSPPVREIASREVSVFVGQEPVPPYAQALSREVSVLVTT
                     PTAPARITQLVVTPSPTGDTVTLSWEGYNQWAEHDVARYDIYMGTQPFSDVTGLTPYK
                     AVPGETFSLTLTGLEPWQDRFFAVVPVDALENSDHTVDYAAAYIIARETVSREFSVFV
                     GGEPEPPYQQALSREVSVLITTPTAPARITQLVVTPSPTGDTVTLSWEGYNQWAEHDV
                     ARYDIYMGTQPFSDVTGLTPYKAVPGETFSLTLTGLEPWQDRFFAVVPVDALENFDHT
                     VDYAAAYIIARETFSRELSFFVGGEPESPYQQALTREVSVLVSTAEIPAPVTGLGSTF
                     TAVMSTRAFGAIDLDWTGYNEIGQHDVVRYRIYAGPAYFNDVTNMDPIGYAPADTWQW
                     TLRGLTPLVVYHVAVVAEDALEHWNSEVRSVSAQASIGSLGEVRQLAAACGIDSLRFT
                     WLAPEGADPAANDFLAEYRVYFAGATAPVVLDRSATEYTVSGLLPAHGYPFRITTMNK
                     FGQESGGASLLAATLLEHPANLEAEPFDRMVRLTWDHAQPTELVQWYAVYAADADFAS
                     VAGMTPISLTKAARADLSGLENGRTYYFAVTTVNTAGCEVDAVQTVSAVPNPREPVNP
                     PVAMDDVAATTRDRAVDLPIARLLANDSDPEGDTLILSLPAPASAQGGSVAVAGEYVR
                     YVPPAGFTGVDTFDYVASDSEGNSDSATVTVIVREEESLGLNLISAVCNGDDSIGLVM
                     AGIPGQLYQIWFTADMAAGPWTLMATVPADPNGVVAYTDATAIGVPVRFYRLAEAP"
     gene            complement(4020..5315)
                     /locus_tag="PKK20_09465"
     CDS             complement(4020..5315)
                     /locus_tag="PKK20_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00154.1"
                     /translation="MRNLIVISLAAVALGSLVASAALNVPSDGSDGVLNITANTEIDL
                     SQAVTGTWSDNNSANAGKGIYDPVKWAVVFKYSNVNIAAGATVTFKNHPSRAPVVWLV
                     SDDATIDGEINLDGQTFVPPPGLSEGGPGGFRGGSGNYAPGATDAAGFGPGGGGRRVG
                     SNLYGYYGGAGSYGSVGLNGRPVYGNPSLIPLVGGSGGGGRSESIPFSQGRSGCGGGG
                     AILLACGGTLTISTSGKIQANGGNGLYGSSAHHNYDYPSGSGGGIRLIAHTHAGKGIV
                     QALGGPGYFYGGMGRIRLERVENTSTLQVTPDPSIVPLADGSTPQLWMPDNGPTVRIV
                     SIGGAEPPDDPRAEFGAIGADVVLPQVTTTPVVIETVNVVEASVVSVRVTPRSNGDFT
                     ETVATVTEVLDDDPKTVRWTANVPVKNGYAAMQVKVVRP"
BASE COUNT         1012 a         1864 c         1715 g          993 t
ORIGIN      
        1 gtcatcgacg ggattcacca cagagccgca gagaacacag agaagacctt aatcctcaga
       61 atttcatctg tggctctttg gatgcgctgt cctctgtggt ctctgccgtc ttggcgtctt
      121 ggcgttgaac tcggctcggc ccggagcgaa cgcaaaggcg cagggacccg gtgcgtcagt
      181 gcgtcggtgc gtcagtgcgt cggtgcgtcg gtgcgtcagt gggttgaatc ccctactcga
      241 tggctgtcac tgtcctccca atcgaacatt tcacgatgct gcgcatggtc cgatgctcgg
      301 ggtctggcat tgtgggcctg ttgcatcggg tccatcgatg ctctcattcc tgatctgccg
      361 cccgttgctg ttcagcttcg atcgctctca gtggtcgccg gttcgacgcg cggtgcgccg
      421 gcaggtgccc caccccagca agcccaacgc cgccaacccg gcgcacgcgc ccggctcggg
      481 cacatactcg agttcgagcc cccaggattc gagtcgcgcc tcgccgaccg gcgacaaatc
      541 ggccaggaac agcgtccacg caccgtccgg agtgtggctg atgaacgacg acaggaagcc
      601 cgagcgcgga tcggtgtcga gcacccgctc gggactgctc gtcctcccat ccggctgcca
      661 agtgcccgtc aggggtcctc ccagtggggt gttgtggctg ccttgcaggg tcaagcggta
      721 gttatggaca tccccttgcg cagcggcatc gctgaaggtc acatacaagc cgctgtcggc
      781 ataacccagc gaatcgtcgg tgcggcgccc gggccggttg agcaacacgg cggacacgcc
      841 gtcgtgaatg agcagcgcgt acaggtcgcc tatgtagcct ccgctcagtt cgagagtcac
      901 ctgcaccccg agaatcgaac cataacccac gctgacctgg cgcgtgtcgc ttgcccccag
      961 actcgagccg tccggcaccg tcagatcgag accgtcgaac gtctggaaga atgtgaacgc
     1021 tgcgtgagcc gaagcggcgc caagggtcag gcacgcgaca actgcaagtg cggcaaatcg
     1081 gttcaggcgc cctcctcggg ccgcgcccgg tttcgggccg ggttgggcgg gactcgccgc
     1141 cgatccgacg agcacaggat ttcgaggtgc aagacgcgcg gcacaaagtt gcgtattcat
     1201 agcaagaacc aaagctgatt gcgattgtct taaggcgctt cggccaggcg atagaaccgg
     1261 accggcacac cgatggccgt cgcatccgtg taggcgacaa caccgttcgg atcggccggc
     1321 acggtcgcca tcagcgtcca cgggcccgcg gccatgtccg ccgtgaacca gatctggtac
     1381 aactgaccgg ggattccggc cattacgagc ccaatgctgt catcaccgtt gcacaccgcc
     1441 gagatgaggt tgagaccgag cgattcctcc tcccggacga tcaccgtgac agtggcggag
     1501 tccgaattgc cctcgctgtc gctggccaca tagtcaaacg tatccacgcc cgtgaagccg
     1561 gcaggcggca catagcgtac gtactcgccg gctaccgcca cgctgccgcc ttgtgccgac
     1621 gccggggccg gcaggctgag gatcaatgtg tccccttccg gatcgctgtc attcgccaaa
     1681 agccgcgcaa tcggcaggtc aaccgcacgg tctcgagtcg tcgccgccac atcgtccatc
     1741 gccaccggcg gattgaccgg ctcccgcggg ttcggcacgg cgctcaccgt ctgcacagcg
     1801 tccacttcgc agccggccgt gttgaccgtg gtcaccgcga agtagtaggt ccgcccgttt
     1861 tcaagcccgc tgaggtccgc ccgcgccgcc ttggtcaggc tgatcggcgt catccccgct
     1921 accgatgcaa agtccgcgtc cgccgcatag accgcatacc actggaccag ctcggtcggt
     1981 tgcgcatgat cccaagtcaa ccggaccatg cggtcaaagg gctcggcttc gagattcgcc
     2041 ggatgctcga gcagggtcgc cgccaacaag gacgccccgc cgctttcctg tccgaacttg
     2101 ttcatcgtgg tgatccggaa cggatacccg tgcgcgggca acaaccccga gacagtgtac
     2161 tcggttgcag accggtcgag cacgaccggc gccgtcgcgc ccgcgaagta aacccggtac
     2221 tcggccagga aatcgttcgc cgccgggtcg gcgccctccg gagcaagcca ggtgaaccgg
     2281 agcgaatcga tcccgcacgc cgccgccaac tgccgcactt cgcccaacga cccgatcgat
     2341 gcctgggccg atacggagcg gacctccgaa ttccagtgct cgagggcgtc ctcggccacc
     2401 acggccacgt ggtagacgac cagcggtgtc aggcccctca aggtccactg ccaggtgtcg
     2461 gccggcgcgt aaccgatcgg gtccatgttg gtgacgtcgt tgaagtaggc cggcccggcg
     2521 tagatgcgat agcgcaccac gtcgtgctgt ccgatctcgt tgtagccggt ccaatcgagg
     2581 tcgatcgccc caaacgcacg cgtcgacatg acagctgtga acgtgctccc caatcccgtc
     2641 accggcgcgg gtatctcggc cgtagaaacc agcacgctga cctcacgcgt cagggcctgc
     2701 tggtacggcg actccggctc accacccacg aaaaacgaca actccctcga aaacgtctcc
     2761 cgcgcaataa tgtacgccgc cgcataatcc acggtgtgat cgaagttctc gagtgcatcg
     2821 accggcacga cggcgaagaa gcggtcctgc cacggctcga ggccggtcag cgtaaggctg
     2881 aaggtctcgc ccggcacggc cttgtatggg gtcaacccgg tcacgtcgct gaagggctgc
     2941 gttcccatgt agatgtcgta gcgcgccaca tcgtgctcgg cccattggtt gtacccttcc
     3001 catgagagcg tcacggtgtc gcccgtcggg ctgggtgtga cgaccagttg cgtgatccgc
     3061 gccggggcgg tcggtgtggt gatcagcacg ctcacctcgc ggctgagggc ctgctggtac
     3121 ggcggttcgg gttccccgcc cacaaagacc gaaaactcgc gcgacaccgt ctcgcgcgcg
     3181 atgatatacg ccgccgcata gtcgacggtg tgatcggagt tctcgagtgc atccaccggc
     3241 acgacggcga agaagcggtc ctgccacggc tcgaggccgg tcagcgtaag gctgaaggtc
     3301 tcgcccggca cggccttgta tggggtcaac ccggtcacgt cgctgaaggg ctgcgttccc
     3361 atgtagatgt cgtagcgcgc cacatcgtgc tcggcccatt ggttgtaccc ttcccatgag
     3421 agcgtcacgg tgtcgcccgt cgggctgggt gtgacgacca gttgcgtgat ccgcgccggg
     3481 gcggtcggtg tggtgaccag cacgctcacc tcgcggctca gggcctgcgc atacggcggc
     3541 acgggttcct gtccgacgaa aaccgacacc tcgcgcgacg caatctctcg aaccggaggc
     3601 gattgcaccc cgccgacaaa aaccgagacc tcgcgtgaaa aggtctcgcg cagcagcagg
     3661 tccccttgcc cgaaggcgca cggcaaggcc agccacccgg ccagcgccgc catccagagc
     3721 cgcatcgaga agcgccgggc ggattgcgat tccgattgtg cccgggccgg catgccacag
     3781 tgcattggtt tcatgttacg agttgctact gcctggtcgg ttgaatgcgc tacacagccc
     3841 cctcacctcg gccctctccc atccgacctg cctgccgaag cctgggcgca ggcaggtggg
     3901 agagggtgct cctgcaactg cggggcgggt gagggtcagg gtgtagccca tcccggtgat
     3961 tcctcagtaa accgcatagc ccggatcgat cacagcctcc gcgctgaggc taatgcgcgc
     4021 taagggcgga ccaccttgac ctgcatggcc gcatacccgt tctttaccgg cacgttcgcc
     4081 gtccaacgca cagtcttcgg atcgtcatcg agcacttccg tcacggtcgc cactgtttcc
     4141 gtaaaatcgc cgttcgaccg cggggtgacg cgcacggaga caaccgaagc ctccaccaca
     4201 ttcacggtct cgatcaccac cggcgtggtc gtcacctgcg gcaggacaac gtccgcacca
     4261 atggccccga actccgcccg cggatcgtcc ggaggctccg cgccgccaat cgaaacgatg
     4321 cggactgtcg ggccgttgtc cggcatccaa agctgcggcg tcgacccatc ggcgagcggc
     4381 actatgctcg gatcaggagt cacttgaagc gtgcttgtgt tctccactcg ttcgagacgg
     4441 attcgaccca tgccgccata aaagtacccg gggcccccaa gggcctgcac gatccctttg
     4501 ccggcgtgcg tgtgggcgat caatcgaatg ccgccaccgc tcccgctcgg gtaatcatag
     4561 ttgtggtgcg cagacgaacc atatagaccg ttaccgccat tggcttgaat cttgcctgat
     4621 gttgagatgg taagggtccc gccgcaggcg agcaggatcg ccccaccccc gccgcaacca
     4681 ctgcgcccct ggctgaaagg gattgattca gaccttccac ctccgcccga tcctccaacc
     4741 agcgggatca acgacgggtt gccgtagacc gggcggccgt ttaaacccac cgaaccgtaa
     4801 ctgccggcgc cgccatagta gccgtaaagg ttgctgccca ctcgacggcc accgccgcct
     4861 gggccgaatc cggccgcatc agtcgcccca ggtgcgtagt tccccgagcc tcctctgaat
     4921 ccgcccggcc ccccctcgga caaccccggc ggcggcacga aggtctgccc gtcgaggttg
     4981 atctccccgt caatggtcgc atcatcgctg actagccaga cgacgggtgc gcggctgggg
     5041 tggttcttga acgtgacggt ggcgccggcg gcaatgttca cgttcgagta cttgaagacc
     5101 acggcccatt tcacaggatc gtagatgcct ttgccagcgt tggcgctgtt gttgtcgctc
     5161 cacgtccccg tgacggcctg agacaggtcg atctcggtgt tggccgtgat gttcaggacg
     5221 ccgtcgctgc cgtcggacgg aacgttcaga gccgccgagg caaccaggct gccaagcgcg
     5281 acagccgcga gtgatatgac aatgagattt ctcataggga aggtgctgcg accggtggca
     5341 gccgaggttt tggatttccg gacgtaatac acggattgtc tctgtcgggc ggccacacac
     5401 cgcccgtttg ctctttcctc tgccatcgta agggaaaggt tacagaaaaa gtgcgggaag
     5461 tttggatgag cgagggcatc caaacccctt ctctgcgtgc tctgtgtctc tgtggttcaa
     5521 ttcgggaacg gtgacagcca tcgacgggat tcaccacaga gacgcagaga acacagagaa
     5581 gacc
//
LOCUS       DAOQRO010000243         5537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15031_length_5537_cov_4.004925, whole genome shotgun sequence.
ACCESSION   DAOQRO010000243 DAOQRO010000000
VERSION     DAOQRO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15031_length_5537_cov_4.004925"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PKK20_09470"
     CDS             <1..1102
                     /locus_tag="PKK20_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00155.1"
                     /translation="SWTTTRTATHPQILLTRRKGFKGAFSQDEAQLFAVSMGLTAQEA
                     KTKLRYVAGADTTKARKKLGFADKEAPLLNVLIYPISDEEAHNFDGDLASLNNVIRTK
                     MLGDKSAGIRGILDDLLKRIGPDDVVVLSSDHGFKELLQGDGVAVAEAEAKSAGMTME
                     ESIRWRYVEGFAPAAMPDAVKIAVGSQDVWMALGRRWFMREGTKTAPRYSHGGLSLAE
                     AVVPGVVLRRVTEKTARAELLDLPTVLQAEEDAVAELPFTVRNTGNCDIEFDVRVINN
                     LGQELFQQQAKLAPATTAKLTASVTARYRETPAREPDPAATVSAVTLRLRHTNLNGTW
                     QEALDGRASIPVKIKPKAVKFDTDALKGFDDV"
     gene            1115..2578
                     /gene="brxL"
                     /locus_tag="PKK20_09475"
     CDS             1115..2578
                     /gene="brxL"
                     /locus_tag="PKK20_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HNV00156.1"
                     /translation="MADEKVNEIEPSLGALDEKIARTLGDRVILKPLTRWNEAYKEFP
                     RYVMEYLVSRYVEPSNPVDGQKKIDRILSEHYSESAKKELIKSRIKERGQYTLLGQLS
                     VRLDQSRDHYWANVPALGDDTVRVAQKVLAQYGDVLLTSGAWGTMEIEYDGTYEIKSR
                     KFPFYIREFTPLQYTRLNLDDFVDKRVEFTADEWLDLLIQSIGFNPAKFERRVKLLML
                     LRLVPFVEANFNLIELGPRETGKTYTFRNTSNRAFVVSGGKATPATLFYNKASRKLGV
                     IGLKHLVFFDEIANTNFDDAEASISVLKDYMQTGKFSRGDQEFSAPCSVVLGGNIDTN
                     LELKQPEVGYKHLFAVLPEELQDPAFLDRIHAYLPGWEMPKIRPENYATGYGLLTDYM
                     AEIFAELRRRNFQTHVNAWVDFGNMTGRNQDAIKKTAAGLLKLLHPHRSPDTLASDEI
                     HPLMELAVEMRRRVTDQLAKILPTEFSNVQYGFKLTR"
     gene            complement(2562..2702)
                     /locus_tag="PKK20_09480"
     CDS             complement(2562..2702)
                     /locus_tag="PKK20_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00157.1"
                     /translation="MKASWATGDAQEPIGWLFADNAEAAVAEVARDGKAWVEALPIIE
                     SA"
     gene            2879..5029
                     /locus_tag="PKK20_09485"
     CDS             2879..5029
                     /locus_tag="PKK20_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00158.1"
                     /translation="MWTLIRIGLALGLLAGGVFWPCQAGPFQSGEVWIRQVYIHGGTL
                     TSVAEQRVRRHMRASAQYAKSVFHQVGIRLQVSEPALFASSDSQGLPLPVGMTDADFV
                     ALLAWYDREHRSPGVVPVFFVDHRASHPSGVACSEDKAVELGAPTPGVIIDHPSDWAL
                     VWQYSADELAHQLGHVLLNRHRYAPFGAENAHLTVAGALMCSAGPPVSRKRPAYQAAY
                     DQDDSVDIIDFDWVRDLDSGARASQVEGLYTGSSLVRKTETLAWGASVGLGQYTADDP
                     SPTPPSVLSIAYGADASGLAFGARLSVPVTRAIREIETGSSVPVTRVESTGATVEVSA
                     WSRRKTESMIEVCLDAVFNGLSATDKPSGFSPVPLLSFSDALTVTLGVIPEVEGPAQT
                     RVYVQRTPSDDMRELAPAEYVFAGATNLVVEKDLLGEGGVLRLRFTVPDLTAPLWFGG
                     DMNNDGAFNLDDILRLAARLGNEPSPFDMTGDGRVSLDDLSKLVRERIRTKLGDTNLD
                     GLVDERDFDIVKTNWMAAGNWPNGDFDGDRLVSETDLAILSANWDGRPMRWQGYPIIR
                     HTEIPVSILPITAARLDWASVADDRYRFQTSTDLAVWSEDGLPVIGTGGPLARWVQVR
                     TEPARYFRLAATDDSDSRLGVGAVGAAFRASWASVDAQWYQVQAWLPLGSHDESAWVN
                     QGAPVRGDGSLLTAIVPIGLADANRAGCRVLLVP"
     gene            complement(5176..>5537)
                     /locus_tag="PKK20_09490"
     CDS             complement(5176..>5537)
                     /locus_tag="PKK20_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV00159.1"
                     /translation="SVTFEAALRNERGQPREVDLFVHDNGPGLPPEAILSVLDPLIHR
                     TGAAQGSGLSLMACYFLVYHHGGKVAIEPKTGTGLSLRITLPLEPPAVNPQIESEAFL
                     LRAMTNERLWDRLLAGE"
BASE COUNT         1044 a         1575 c         1851 g         1067 t
ORIGIN      
        1 atcttggacg actacccgaa ccgccactca cccacagatt ctgctgacga ggcggaaagg
       61 gttcaagggc gcgttcagcc aggacgaggc gcaactgttc gccgtgagca tgggtctgac
      121 cgcccaggag gccaaaacga agctccgcta cgtggcgggc gcggacacga cgaaggcccg
      181 gaagaagctc ggcttcgccg ataaagaggc tcccctgctg aacgtgttga tctacccgat
      241 ctcagatgaa gaagcgcata acttcgatgg cgacctggcc tcgttgaaca acgtcattcg
      301 caccaagatg ctcggtgaca agagcgcggg catccggggc atcctggatg acctgttgaa
      361 gcggatcggc ccggacgacg tggtagtgct gagttcagat catggcttta aggagttgct
      421 ccaaggagat ggcgtggccg ttgccgaagc cgaggccaag agtgcgggca tgacgatgga
      481 ggagagcatc cgctggcgct acgtcgaagg ctttgcgcct gccgccatgc ccgacgccgt
      541 aaagatcgcg gtgggcagcc aggacgtgtg gatggccctg ggcaggcggt ggttcatgcg
      601 ggaaggcacg aaaaccgccc cgcgctacag ccacggcggc ttgtcgctgg ccgaggccgt
      661 ggtgcctggc gtggtgctgc gccgggtgac cgagaagacg gcgagggcgg agctactcga
      721 tctgccgacc gtgctccagg cggaggaaga tgccgtcgcg gaactgccgt ttacggtgag
      781 gaacaccgga aactgcgaca tcgagttcga cgtgcgggtt atcaacaatc ttgggcagga
      841 gttgttccag cagcaggcga aactggcccc ggcgaccacc gccaagctga cggcctccgt
      901 gacggcacga tatcgggaaa cgcctgcgcg tgaacccgat cccgctgcta cggtcagcgc
      961 cgttacgctg aggctgaggc atacgaatct gaacggaacc tggcaggagg cgctggatgg
     1021 gcgggcctcc attccggtga agatcaagcc caaggcggtg aaattcgaca ccgacgccct
     1081 caaaggcttt gatgacgtat agaaggaaat tttcatggca gacgaaaaag taaacgaaat
     1141 cgagccgtcc ctgggggcct tggacgagaa gatcgcccgg acgctcggtg atcgggtgat
     1201 actcaagccg ctcacgcggt ggaacgaggc ttacaaagag tttccacgtt acgtgatgga
     1261 atacctggtc agccggtatg tggagccgag caacccggtg gacggccaaa agaagatcga
     1321 ccgaattttg tcggagcact actcggagag cgcgaaaaag gaactcatca aaagccggat
     1381 caaggagcgc ggccaatata cgttgctggg ccagttgtcc gtgcggttgg accagagccg
     1441 tgaccattac tgggccaacg tgcccgccct gggtgacgac acggtgcggg tcgcgcagaa
     1501 agttttggcc cagtatggcg acgtgctgct gaccagtggg gcctggggca cgatggagat
     1561 cgaatacgac ggcacctacg agatcaagag ccgcaagttc cccttctaca tccgcgagtt
     1621 cacgccgctc caatacaccc ggttgaacct ggacgatttt gtggacaaac gggtcgagtt
     1681 cactgccgat gaatggcttg acctgttgat tcagtccatc gggttcaatc cggccaaatt
     1741 cgagcgccgg gtcaaactgc tcatgcttct gcgcctggtg ccgttcgttg aagcgaactt
     1801 caacttgatc gagcttgggc cacgcgagac cggcaagacc tacaccttcc gcaacacgtc
     1861 caaccgggcg ttcgtggtct ctggcggcaa ggcgacgccc gccacgctgt tctacaacaa
     1921 ggcatcccgg aaactggggg tcataggatt aaagcacctg gtctttttcg acgaaatcgc
     1981 caacaccaat ttcgacgacg ccgaggcgag catcagcgtg ctcaaggact acatgcaaac
     2041 cggcaagttc agccggggcg atcaggagtt ctccgcgccg tgctcggtcg tcctgggcgg
     2101 caacatcgac acgaacctgg aactcaagca accggaggtc ggctacaagc acctgttcgc
     2161 cgtgctcccg gaggagcttc aagaccccgc cttcctggac cgtatccacg cctatctgcc
     2221 cggctgggag atgccgaaaa tcagaccgga gaactatgcg actggctacg gcctcctcac
     2281 tgactacatg gccgaaatct tcgcggagtt gcggcgacgg aactttcaga cgcacgtcaa
     2341 cgcctgggtg gatttcggaa acatgacggg ccgcaatcag gacgcgatca agaagacggc
     2401 ggcgggctta ttgaagttgc tccacccgca ccgcagcccg gacaccctgg ccagcgacga
     2461 aatccacccc ctcatggaac tggcggtcga gatgcgccgg cgcgtcaccg accagctcgc
     2521 caaaatcctg cccacggagt tcagcaacgt ccaatacggg ttcaagctga ctcgatgatc
     2581 ggcaaagcct cgacccaggc tttgccgtcc cgggcaacct cggccacggc tgcctcggca
     2641 ttgtcggcga ataaccagcc gattggctcc tgggcgtcac cagtcgccca agaggctttc
     2701 atgacaattc atgacaactt ttgcccccgt tttgttcctt cgctccgact tgcctttttt
     2761 ataaacaatt ctaagctgtt ggcgtatggc aagttgcaga ggcatttttg aagaagttgg
     2821 aagcgaatcc gaagtgttcg aatcccttcg ctcgctccaa tcttcatctc ggaatctcgt
     2881 gtggacgctc attcgaatcg gattggcgct cggccttctg gccggtggtg tgttttggcc
     2941 gtgtcaggcg ggcccttttc agagcggtga ggtctggatt cgccaggtct acattcacgg
     3001 gggcactctg acgtccgtag ccgagcagag ggttcggcgg cacatgcggg cttcggcgca
     3061 gtacgccaag agcgtttttc atcaggtcgg tattcggctg caggtgagcg agccggcttt
     3121 gtttgcgtcg tcggattccc agggcctgcc gctgcctgtt ggcatgacgg acgcggactt
     3181 cgtcgcactt cttgcctggt acgatcggga acaccgttcg cccggcgttg tgccggtctt
     3241 cttcgtggat catcgggcgt ctcatccgtc gggtgtggct tgttcggagg acaaggctgt
     3301 agaactcggc gcgccgacac ccggggtgat catcgatcat ccatccgatt gggcacttgt
     3361 ctggcagtat tcggctgacg aactggcgca tcaactgggc cacgtgttgc tgaaccgaca
     3421 tcggtatgcg cccttcggag ccgaaaacgc acacttgaca gtcgcgggcg ccctgatgtg
     3481 cagcgcgggg ccgccggtgt ctcgcaagcg ccctgcgtac caggctgctt atgatcaaga
     3541 tgacagcgtg gacatcatcg atttcgattg ggtccgggat ctggacagcg gggctcgcgc
     3601 aagccaggtt gagggccttt acacgggctc gtcgttggtt cgcaagacgg agaccctggc
     3661 ctggggcgcg agcgtgggtc ttgggcagta caccgccgat gatccgtcgc cgacgcctcc
     3721 gtcggtgctg tcgattgcat acggcgcgga cgcgagcggc ttggcctttg gggcccgcct
     3781 ttccgtcccg gtgacgaggg cgattcggga gatcgagacc ggatcgtctg tgccggtcac
     3841 gcgggtcgaa tcgacggggg caacggtcga ggtttcggcc tggagccggc gcaagacgga
     3901 atcgatgatc gaagtgtgtc tggacgccgt tttcaacggt ctgtcagcca cagacaagcc
     3961 atccgggttt agccctgtgc ctctgttgtc attcagcgac gctttgaccg tgacgctggg
     4021 cgtgattccg gaggtcgagg gtcccgcgca gacgcgtgtg tatgtgcaga ggacgccctc
     4081 ggacgacatg cgcgaacttg cgccggcgga gtatgttttt gcgggagcga cgaatctggt
     4141 ggtggagaag gatcttctag gcgaaggcgg cgtgttgcgc ttgaggttca cggtgcccga
     4201 tctgacggcg ccgctctggt ttggcggtga catgaacaac gacggcgcat tcaatctgga
     4261 cgacatcctc cggctggccg cgcgcctggg caacgagccc tcgcccttcg acatgacagg
     4321 cgatgggcgc gtcagtctgg atgatttgag caagctggtg cgggagagga ttcgcacgaa
     4381 gctcggcgac acgaacttgg atggactggt cgatgagcgg gatttcgaca ttgtcaagac
     4441 gaattggatg gcggcgggga actggcccaa cggggatttc gacggcgacc ggcttgtgag
     4501 cgagacggat ctggccattc tctcggcgaa ctgggatggc cggcctatgc gctggcaagg
     4561 ttatccgatc atccgacata ccgaaatccc ggtgtcgatc ctaccgatca cggctgcgcg
     4621 tctggactgg gcatcggtgg ctgacgaccg ctatcggttc cagacctcga cggatttggc
     4681 ggtgtggagc gaggatgggt tgccggtgat cgggaccggc ggcccactcg cgcggtgggt
     4741 ccaggtgcgg accgaaccgg ctcggtactt caggctggcc gcaacggacg attccgattc
     4801 gaggctgggt gtgggggcgg ttggcgcggc attccgtgcg tcctgggctt ccgtcgatgc
     4861 tcaatggtac caggtgcaag cctggctgcc gctcggttcc cacgacgagt ccgcatgggt
     4921 gaaccagggc gctcctgtgc gtggcgacgg ttcgctgctg acggcgattg tgccgatcgg
     4981 cttggcggat gcgaatcggg cggggtgtcg tgtccttctt gtgccgtgag caggcggctg
     5041 cgtcgggcgg ggtggcgcgc tgcctttccc ggcctgcatg ctggcttctc ttgtgatctt
     5101 cggcctcgcg gtggcaaaaa gtggtgagaa atgcaggcaa gcgcccgcgg ctcgggtggg
     5161 gactcgagtc gcggcctatt ccccggcgag gagccggtcc cagaggcgct cgttggtcat
     5221 ggcgcggagc aggaaggcct cgctttcgat ctgcgggttg acggcaggag gctcgagcgg
     5281 gagcgtgatc cggaggctga ggccggttcc ggtcttgggc tcgatggcga ctttgccgcc
     5341 gtgatggtag acgaggaagt agcatgccat gagggaaagt cccgatccct gggccgcgcc
     5401 cgtccgatgg atcagcgggt cgaggaccga gagaatggct tcgggcggca agccggggcc
     5461 gttgtcatgg acgaagaggt ccacctcgcg gggctggccg cgttcgttgc gcagggcggc
     5521 ttcgaaggtg actgagg
//
LOCUS       DAOQRO010000244         5533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15047_length_5533_cov_3.695144, whole genome shotgun sequence.
ACCESSION   DAOQRO010000244 DAOQRO010000000
VERSION     DAOQRO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15047_length_5533_cov_3.695144"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="PKK20_09495"
     CDS             complement(<1..2287)
                     /locus_tag="PKK20_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNV00160.1"
                     /translation="MKTLRVVWIGLALALGMARLQAVMPTQEEMAACRLWIDRLTAAG
                     EASAAPASIEFAYEDVADSMTRGRSWRGTAYQLGEKVYTHGIAFNATKHLRVRLGRPA
                     QRFTADVGLENNDDTQRGEAMGAGSVTFHVLVKGTEVFASPVMRRKDGPRAIDIPLEG
                     ASEFEIRVKDGGDGRGWDQALWAEAVVTLEDGSRVRLQDLPWADAVGANPFAVSFVYG
                     GINSAGLLPQWPRAVEESSSRPQRLDRTIVRRDPATGLEIRIDAALFADWPAVEWVAR
                     LKNTGSSDTPILEAIQILDEVLPVSVSGQAKLHWAKGAVASFDDFAPAETVLKPGVKL
                     HLQPGGGRSSSQVMPFFNVEGADGGVVAAIGWSGEWAADFVCGVRGQVTMRAGLAQTR
                     LRLRPGEEIRTPSLALVFYTGDRWRGQNLWRQFVLAHHRPHLDGKPLVAPITSGNWGG
                     TRAEVHLDNIAKIIRHDLPVAYYWIDAEWYGTGGWPVNVGRWQVKRDLYPNGFKPLSD
                     ALRKDRRELMLWFEPERVFKGTPWHAERGDWLLDVGGDNLLFNLGIPEARQFLTDFIL
                     SKVDEFGLGCYRQDFNMDPLPFWQKADAPDRQGMSEIRYIEGLYAFWDGLMAGRPGLM
                     IDNCASGGRRIDLETLGRATPFWRSDGPRDAIAHQCHTYGLLAWAPLSATSQDRPGDD
                     YEFRSSMCSALCLNWWHTGDGPGERIPADFPFVWARVTLVQYLGLRDFYYRDYYPLTA
                     YSQARDQWMAYQLDRPDQGRGL"
     gene            complement(2306..3679)
                     /locus_tag="PKK20_09500"
     CDS             complement(2306..3679)
                     /locus_tag="PKK20_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNV00161.1"
                     /translation="MTLTEKILARAAGKAQVQAGENVWVKADILMTHDVCGPGTIGVF
                     KREFGADAKVWDRRGVVIIPDHYIFTADSKSNRNVDILREFVKEQGITYFYDVIDDPQ
                     GAWKFDPSRGVLKRQYGRRYAGVCHTALAEKGHTRPGEVLFGTDSHTCMAGAFNQFAT
                     GIGNTDAGFILGTGRLLLKVPETMRFRLEGQLEPGVMAKDIILHLIGQIGSDGATYRA
                     LQFDGPGVATLAMDDRMTIANMAIEAGGKNGIFPFDSRTAEYVEARTRLNGTKAAYEP
                     VEADTDQQFVFETTLDLSKIEPTVACPPDPGRRKAAKELGHIRLDRAYIGSCTGGKAS
                     DFVAFARVVRGRRVSIDTFGVPATPGVVRDLQETYWGGRSIWDLLLDAGVQMTENAGC
                     AACLGGPVDTFGRMNAPLKCISATNRNFPGRMGHKESQVFLASPATVAASAIEGRIVD
                     PREYWRS"
     gene            3786..4658
                     /locus_tag="PKK20_09505"
     CDS             3786..4658
                     /locus_tag="PKK20_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNV00162.1"
                     /translation="MKLHPIHWLSLALTGLLAIPAAAGEPAARAQDLCVMTFNLRYAS
                     ATPPNAWPQRRPVMKACIEQSAPDVIGTQEGVYHQLKDLAADLPAYAWIGTGRDGGSR
                     GEFMAVFYRKERLEPIEFDHFWLSDTPDVIGSSTWGNTNRRMVTWVRFRDLASGREFY
                     FWNTHLDHALQPAREKGAALIRQRIDDLKTKLPILLVGDFNAQAGVNKAYDILVKEAG
                     LEDTWLLAAERRNDGINTFHSFTGPRQGDVRIDWILGRGIERVRASEIVTFSQDGQYP
                     SDHFPVVAWLTLES"
     gene            4655..5254
                     /locus_tag="PKK20_09510"
     CDS             4655..5254
                     /locus_tag="PKK20_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNV00163.1"
                     /translation="MKPNPKPRVALAVVSLVLGIVSVLFCIGILTGIPAIITGHVVRR
                     KVRVNPIGYRGARLGMAGLVLGYLSLLQTFLLVIALQPALGKAKLQMEYFQCANNLRQ
                     IGMAVQIYATVNDGVFPQTADQFGPHMGFSRFLICPADAGFRNRKIDSKDFSQTSYIF
                     TLGGASAATLAEVIARCPFHNMTLTADGAVHRPDEPPQP"
BASE COUNT         1015 a         1925 c         1638 g          955 t
ORIGIN      
        1 ccagtccgcg cccctgatcc gggcgatcga gttgataggc catccactga tcgcgcgcct
       61 gcgagtaggc ggtcagcgga tagtaatcgc ggtagtagaa atcgcgcaga cccaggtatt
      121 ggaccagcgt cacccgcgcc caaacgaacg ggaagtccgc tggaatccgc tccccaggcc
      181 cgtcgcccgt gtgccaccaa ttgagacaga gggcgctgca catgctgctc cgaaactcgt
      241 agtcgtcgcc gggacggtcc tggctggtcg cactgagcgg cgcccaagcg agcaatccgt
      301 acgtgtggca ttggtgcgcg atcgcatcgc gcggtccgtc gctgcgccag aaaggcgtgg
      361 cgcgcccgag cgtttcgagg tcgatccggc gtccgccgct ggcgcagttg tcgatcatca
      421 gcccggggcg gccggccatc agcccgtccc agaacgcgta gaggccctcg atgtatcgga
      481 tttcgctcat cccctgccgg tcgggcgcgt ccgctttctg ccaaaacggc agcgggtcca
      541 tgttgaaatc ctgccgataa cagcccagcc cgaattcgtc caccttgctc agaatgaaat
      601 cggtgagaaa ttgcctggcc tcaggaatcc cgaggttaaa cagcaggttg tcgccgccca
      661 catccagaag ccagtcaccc cgctccgcgt gccagggcgt tcccttgaac acccgctccg
      721 gctcgaacca caacatgagt tcgcgccgat cttttctcag cgcgtcgctc agcggcttga
      781 atccgttcgg atagagatcg cgtttcactt gccagcggcc gacgttgacc ggccagccgc
      841 cggtgccgta ccactcggca tcgatccagt agtacgccac gggcagatcg tgccggatta
      901 tcttggcgat gttgtcgaga tggacctcgg cgcgcgtgcc gccccagttg ccagaggtga
      961 tgggggccac gagcggcttg ccgtcaaggt gcggacggtg gtgcgccaac acgaattggc
     1021 gccagagatt ctgcccgcgc caccggtctc ccgtgtaaaa gaccagcgcc aggctcggcg
     1081 tgcgaatctc ctcgcccgga cgcagccgca gacgggtctg cgccaggccg gcacgcatgg
     1141 taacctggcc gcgcacgccg cacacgaagt ccgcggccca ttcaccgctc cacccgatcg
     1201 ccgccaccac gcccccgtcc gctccctcga cattgaagaa cggcatcacc tggcttgacg
     1261 accgaccgcc gccgggctgc agatgaagct tcacgcccgg tttcagaacg gtctccgccg
     1321 gcgcaaagtc gtcgaatgaa gccaccgcgc ctttggccca atgcagcttg gcctggcccg
     1381 acacggacac aggcagaacc tcatcgagga tttggatcgc ttcgaggatc ggcgtgtcgc
     1441 tcgaaccggt gttcttgagg cgcgccaccc actcgaccgc gggccagtcc gcaaaaagcg
     1501 ccgcgtcgat gcggatttcg agtccagtgg ccggatcgcg ccggacgatc gtccggtcga
     1561 gacgctgcgg gcggctcgat gattcctcga ccgcgcgcgg ccattgcggc agcaaacccg
     1621 cgctgtttat gccgccgtag acgaaggaga ccgcgaatgg gttggcaccc accgcatccg
     1681 cccacggcag atcctgcaaa cgaacccggc tgccgtcttc gagggtcacg acggcctcag
     1741 cccacaatgc ctgatcccag ccccgcccat cgccgccgtc cttgacgcga atttcgaact
     1801 cggacgcccc ctcgagcggg atgtcgatgg cgcggggccc atccttgcgc cgcatcacgg
     1861 gcgacgcgaa aacctccgtc cccttcacca gcacatggaa agtgactgaa ccggcgccca
     1921 tcgcctcgcc gcgctgggtg tcgtcgttgt tctcgagccc cacatccgcg gtgaacctct
     1981 gcgccgggcg ccccaaacgc acgcgcagat gcttggtggc gttgaacgcg atgccgtgcg
     2041 tgtagacctt ctcgcccagt tgataggcag tgccccgcca cgatcgaccg cgggtcatcg
     2101 agtcggcgac gtcctcataa gcaaactcga tcgacgccgg cgccgcggag gcctcgccgg
     2161 ccgccgtcaa ccggtcgatc caaagacggc acgcggccat ctcctcctgg gtcggcatca
     2221 cggcctgcaa tcgcgccatg cccaatgcca gagcgagacc gatccagaca actcgcaatg
     2281 tcttcatgtt caccaatcaa agaccctacg agcgccaata ctcgcgcggg tcgacaatcc
     2341 gcccttcaat cgcgctggcg gcgacggtgg ccggcgaagc caggaagacc tggctctcct
     2401 tgtggcccat gcgcccggga aaattgcgat tggtggcgct gatgcatttg agcggcgcgt
     2461 tcatgcgccc gaaggtatcg accgggccgc ccaggcaggc ggcgcatccg gcgttctcgg
     2521 tcatctgcac cccggcatcg agcaacaggt cccagatgct tcgcccgccc cagtaggtct
     2581 cctgcaggtc gcgcaccaca cccggagtcg ccggcacgcc gaaggtgtcg atgctcaccc
     2641 gccgtccgcg aaccacgcgg gcgaacgcga cgaaatcgct cgcctttccg cccgtgcagg
     2701 agccgatgta ggcgcgatcg agccggatgt ggcccagttc cttagccgcc ttgcgccggc
     2761 ccggatccgg cggacacgcc acggtcggct cgatcttgga caaatcgagt gtggtctcga
     2821 agacaaactg ctgatccgta tccgcctcga ccggctcata ggccgccttg gttccgttga
     2881 gacgcgtccg agcctcgaca tactcggccg tccgactgtc aaaggggaag atgccgttct
     2941 tgccaccggc ttcgatggcc atattggcga tcgtcatgcg atcgtccatg gccagcgtcg
     3001 ccactcccgg cccgtcgaac tgcagcgccc ggtaggtggc gccgtccgaa ccgatctggc
     3061 cgatcagatg caggatgatg tccttggcca tcacgcccgg ctcgagctgg ccttcaagcc
     3121 tgaaccgcat ggtctcgggc accttgagca acagccggcc cgtgcccaaa atgaaccccg
     3181 cgtcggtgtt cccgatcccc gtggcgaact ggttgaacgc gcccgccatg cacgtgtgcg
     3241 agtcggtgcc aaacaggact tcgcccggac gcgtgtggcc tttctcggcc agtgcggtgt
     3301 ggcacacccc cgcatagcgg cgtccgtact gccgcttgag aacgccccgg ctcggatcga
     3361 acttccaggc gccttgggga tcgtcgatga cgtcgtaaaa gtaggtgatc ccctgctcct
     3421 tcacgaactc tcggaggatg tcgacgttgc ggttcgattt cgagtcagcc gtgaagatgt
     3481 aatggtcggg aatgatcaca acgccgcgcc gatcccagac cttcgcatcg gccccaaact
     3541 cgcgcttgaa cacgccgatc gttccgggcc cgcacacgtc gtgtgtcatc agaatgtcgg
     3601 ccttcaccca gacgttctcg cccgcctgaa cctgcgcctt gccggccgcc cgcgccagaa
     3661 tcttctcagt caacgtcata acacgaacct gaattgtatc ggaccggcgc tcagcgcgca
     3721 actcgggaac cgcagcccac agaaaaggcg tgcagaaacc ggtcgagtca ccccatgctg
     3781 tgtccatgaa acttcacccc atccattggc tcagcctcgc gctcaccggc ctcctcgcca
     3841 tcccggctgc cgccggagaa ccggctgccc gcgcccagga cctctgcgtc atgacgttca
     3901 acctccgcta tgccagcgcc acacccccca acgcctggcc tcagcgccgt ccggtcatga
     3961 aagcatgcat cgaacagtcc gcccccgatg tcatcggcac gcaagagggg gtctaccacc
     4021 aactcaagga cctcgccgcc gatctgccgg cctacgcctg gatcggcacc ggacgcgacg
     4081 gcggcagccg cggcgagttc atggccgtgt tctatcgcaa agagcgattg gaaccgatcg
     4141 agttcgatca tttctggctc tccgacaccc cggatgtgat cggttcgagc acatggggta
     4201 acaccaaccg ccgcatggtg acttgggtcc gatttcgcga cctggcgtcg ggacgcgagt
     4261 tctacttctg gaacacgcac cttgaccacg ccctccagcc ggcgcgcgag aaaggcgcgg
     4321 ccttgattcg gcagcgcatc gacgatctca agaccaaatt gcccatcctg ctggtcggag
     4381 atttcaatgc gcaggcgggt gtgaacaagg cctacgacat cctcgtcaaa gaagcgggcc
     4441 tggaagacac atggctgctg gccgccgaac gccgcaatga cggcatcaac acgttccact
     4501 ccttcaccgg cccccgccag ggagacgtcc gtatcgactg gatcctgggg cgcggcatcg
     4561 agcgcgttcg ggccagtgaa atcgtcacgt tctcccaaga cggacaatac ccgagcgacc
     4621 acttcccggt ggtcgcgtgg ctgaccctcg aatcttgaag ccaaatccca aaccgcgcgt
     4681 cgccctggca gtcgtcagcc tcgtcctcgg gattgtctcg gtcctcttct gcatcggcat
     4741 cctgaccggc attccggcga tcatcaccgg tcatgtggtc cggcgaaagg tcagggtgaa
     4801 tccgatcggt taccgcggcg cgcggttggg gatggccggc cttgtcttgg ggtacttgag
     4861 cctgctgcag acgttcctgc tcgtcattgc cctgcagccc gctttgggaa aggccaagtt
     4921 gcagatggaa tactttcagt gcgccaacaa cctgcgacag atcggcatgg ccgtccaaat
     4981 ctatgccacg gtcaatgacg gcgtcttccc ccaaaccgcg gatcagttcg ggccgcacat
     5041 gggattctca agattcctga tctgccccgc cgatgccggt ttcaggaacc ggaagatcga
     5101 ctcgaaggat ttcagccaga caagctacat cttcacactc ggtggcgcct cggcggccac
     5161 gctcgccgag gtcatcgccc gatgcccgtt ccacaacatg acgctgaccg ccgatggcgc
     5221 cgttcatcgg cccgacgaac ccccgcagcc ttgaaccctg gacggtggta aaccgccgcg
     5281 cttctcgcgg cgcgcatacg cgtcaaccta aggcaaccac gacatcgctg ctcccagaaa
     5341 aagcgcggta gatcttatga ggttgacgcg tgtgcgcgcc ctcgggagcg gtcctggatc
     5401 cagacaatca cggtcatcaa tcagggcctc gggtaactcg gccctccgag acgtctcgcg
     5461 aggttcgagg cggggcactg aaagcgagcc gcgcctgacc caggctggag acgggacgcg
     5521 cctcaagccg gcg
//
LOCUS       DAOQRO010000245         5499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15209_length_5499_cov_5.584864, whole genome shotgun sequence.
ACCESSION   DAOQRO010000245 DAOQRO010000000
VERSION     DAOQRO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15209_length_5499_cov_5.584864"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKK20_09515"
     CDS             complement(<1..223)
                     /locus_tag="PKK20_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00164.1"
                     /translation="MKTKAISCVLLAAVGAALTLTPPTRALPAAEKEKEKATYLGIAA
                     GEVDDTLREQLKLGRGAGIRVEAVVPGSPA"
     gene            complement(268..786)
                     /locus_tag="PKK20_09520"
     CDS             complement(268..786)
                     /locus_tag="PKK20_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00165.1"
                     /translation="MTATNDPLDDIAAELERLRPIQASDRLTRRLEQELESLPPSITA
                     RAVLPVRFRWVPVAVAAGLAFLAIVTVTWQARLRRPVAGVASSALENNRAEPAAGRMP
                     SAQALRCVDLANYLLTAEEDGLVYASETAPWRKVRWRYLSASEWRNDQEHTTFQVLVP
                     REEVVLLPVNIH"
     gene            complement(758..1426)
                     /locus_tag="PKK20_09525"
     CDS             complement(758..1426)
                     /locus_tag="PKK20_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV00166.1"
                     /translation="MRELTMQPREMNGEWRDLWAEYAPRLLLFARQQTPRLSDAEDIV
                     QDAFVRYWRAQQANPSLSPDVLFVMIRRIAIDYARKNHGRFVRESEAGALSGPAEVWF
                     VDPAEEQERKEEIEKALRSLPAEQREVLVLKVWGELTFERIGEMLEISPHTAASRYRY
                     GLNQLKRVLSGKQSTALDGHSSLAPCISPASTGASQTPACAASEGQSTSTQHRYDCHE
                     RSAR"
     gene            1779..2033
                     /locus_tag="PKK20_09530"
     CDS             1779..2033
                     /locus_tag="PKK20_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNV00167.1"
                     /translation="MGILSWIVLGLIIGALAKLIMPGKDPGGVIVTILLGIAGGFVGG
                     YLGSIAGLGRVTGFDIRSLLLALGGSIVLLILYRIFKKKA"
     gene            2380..3786
                     /locus_tag="PKK20_09535"
     CDS             2380..3786
                     /locus_tag="PKK20_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNV00168.1"
                     /translation="MAPDAFRRHGHAVVDWIADYLERVDSYPVLSQVAPGQIRASLPP
                     SPPAQGEPFEAMLADVEKLILPGITHWQSPSFFAFFPGNNSGPSILGDLLCSGLGVQG
                     MLWTTSPACTELETHVLDWVVDILGLPAHFKSDAAGGGVIQDSASSAAVCALLAARER
                     ATQGESNRRGCDGRLVAYGSTQTHSSIEKAMGVVGMGRDNLRLIDVDDSFAMRPDLLL
                     AQIRRDRAAGLTPCFACATVGTTSSNAVDPLPAIGRICREEQLWMHVDAAMAGTAAVC
                     PEYRFLQDGLDAADSYCFNPHKWMFTNFDCDCFFVADRAALIRALSILPEYLRNQATQ
                     SGAVIDYRDWHVPLGRRFRALKLWFVIRHYGVEGLRHHVRRHIELAHAFARWVRADDR
                     FELAVPPPLNLVCFRHRGGDDVNQRLMDRLNATGRLYLTHTRLGGRLTLRLCVGQTCT
                     EERHVRAAWDLIRQSVES"
     gene            complement(3960..5051)
                     /gene="mqnC"
                     /locus_tag="PKK20_09540"
     CDS             complement(3960..5051)
                     /gene="mqnC"
                     /locus_tag="PKK20_09540"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNV00169.1"
                     /translation="MDDNDAPLDGLLQKVWDGRRVDAAEALRLYRLPLAELGLVANRR
                     RELAKAGAYDGRGNEIVTYIVDRNINYTNICDVRCKFCAFFRREGQPDAYVITPEEMD
                     RKIEETAALGGTQILLQGGHHPTLPLSWYLDLMARIKARFPRINIHGFSPSEFVHFRK
                     VFQLPLDEILRQFRAAGLGSIPGGGAEILVDRVRRAVSPRKAMTDEWLEVMDTAHRLG
                     LRSSATMMFGHVETIEDRIEHLDRLRRQQDATGGFTAFIGWTFQPENTRLEARMAGSC
                     DYLRTQALARIYLDNFENVQSSWVTQGPEIGQVALKFGANDLGSIMIEENVVAQAGAT
                     FRMTADDMRRLIRDIGYEPRQRDNWYRLV"
BASE COUNT          964 a         1776 c         1698 g         1061 t
ORIGIN      
        1 ctgccggact gccgggcacg acagcctcga cgcggatacc ggctcccctg cccagtttga
       61 gttgttcgcg cagggtgtca tcgacttcgc cggccgcaat gcccagatag gttgcctttt
      121 ccttctcctt ctctgccgcc ggcagggccc gggtgggcgg cgtgagggtg agggcggcac
      181 ccacggccgc cagcagaacg cacgagattg cttttgtttt catagtattc acgaatcgac
      241 gaatcgtaac ggggtggacc tttggatcta atgaatgttg acgggcagaa gcacgacttc
      301 ctcgcgcggc accaggacct ggaacgtcgt gtgctcctgg tcattgcgcc attcgcttgc
      361 ggagagatac cgccatcgaa ccttccgcca cggcgccgtc tctgacgcgt aaaccaggcc
      421 atcctcctcg gcagtcagaa ggtagttcgc cagatcgaca cagcgcaaag cctgtgcgga
      481 cggcattcgc cccgccgccg gttccgcccg gttgttttcg agggcactcg aggcgacgcc
      541 cgcaacggga cgccgcagtc tcgcctgcca agtgacagtg acgatcgcca ggaacgccag
      601 gccggcagct acagccaccg gaacccaacg aaagcggacc gggagcacag cacgggccgt
      661 gatagatggg ggcagcgatt cgagttcctg ttccaaccgg cgggtcaacc tgtcgctggc
      721 ttgaatgggt cgcaggcgct cgagctcggc ggcaatatca tctagcggat cgttcgtggc
      781 agtcatagcg atgctgtgta cttgtcgatt gcccttcact cgccgcgcac gccggtgttt
      841 gactcgcccc ggtcgaggcg gggctgatgc atggggcgag ggaggagtgc ccgtcgaggg
      901 cggtgctctg tttgccagac aacactcgtt tcaactggtt caggccatag cgatagcgcg
      961 aggcggcggt gtgcggtgaa atctcgagca tctcgccaat ccgttcgaat gtcagttcgc
     1021 cccatacctt gagcaccagg acctcgcgct gttcggccgg caaggaccgc agcgctttct
     1081 cgatttcctc cttgcgctcc tgctcttcgg cgggatcgac gaaccagacc tcggccgggc
     1141 cggacagcgc gcccgcttcg gactcccgga cgaagcgccc gtggttcttg cgcgcgtagt
     1201 cgatggcgat tcttcggatc atgacaaaca gcacgtcggg tgagagcgac ggattggcct
     1261 gctgcgctcg ccagtaccgc acgaaggcgt cctggacaat gtcctcggcg tcgctcaggc
     1321 gaggcgtctg ctgacgggcg aagagcagga gtcgcggggc gtactccgcc cacaaatccc
     1381 gccactctcc gttcatctcg cgcggctgca tcgtgagttc tctcaccttt accctctata
     1441 gcgtatgtcg acgagggaat tgtctaaagc ggcggtagac cgccgcactc tcccgccaaa
     1501 gagggtctgc agctttcatc gccggagacg aggacgtccc cacaccaacg ccttcgagtg
     1561 cgcgaacaga gcacggcagt ctgcacgcca tgcctgcgcc gcaaagcgcc gcggcctgtc
     1621 tgccgtgtcg ggcacggcac aggcaggcgc ggcgggcagg ccccacacgg cgtaaactcg
     1681 attcccaccg acaatctctc ctgacacact gacgcagcgg ttctcttgac aatttctctc
     1741 ctttcgggtg taaccgcagg aacgagaaaa ggtctatcat gggcatatta tcctggatcg
     1801 ttctgggttt gatcatcggg gcgctcgcca agttaatcat gccaggcaag gaccccggag
     1861 gcgtgatcgt cacgatcctg ctcggcatcg ccggcggatt cgtgggcggg tatctgggct
     1921 cgatcgcggg cctgggcagg gtgaccggat tcgatatccg cagtctgttg ttggcacttg
     1981 gcggatcaat cgtgctgctg atcctctatc gcatcttcaa gaaaaaggca tagcagggtt
     2041 cgcttcggac aaggggacgt tcaacaagag cccggccgcg gactcaagcc ggtcgttgtc
     2101 ggcaacagca gttctcgttg tccggcgaga accgctgaag cctggattcc caaccacgtc
     2161 cgacgcttcg gcgcagtgtt cggtgtacca cctgcaggcg gctcgtggcc aaaatgagaa
     2221 ccgctgtgtc ggcgatgggt cccgccttca gacggcagtc aagcacatcg gcacacccca
     2281 tgccccggcc cggcagccgg tgtgtgtctc cagtttcccg ctggacgcac cccggtggtt
     2341 tgggattgca tggagcccat gccgacaact tcctatcaca tggcgcccga cgccttccgc
     2401 cggcacggtc atgcggttgt ggactggatc gcggactacc tcgagcgcgt cgactcgtac
     2461 ccggttcttt cgcaggttgc gcccgggcag atccgcgcca gcctgccgcc gtcgcctccg
     2521 gcccagggtg aaccattcga ggcaatgctg gcggacgtcg agaagttgat cctgcccggc
     2581 atcacgcatt ggcaatcgcc gagtttcttc gccttcttcc ccgggaacaa ctcggggccg
     2641 tcgatcctcg gagacctgct ctgctcgggc ctgggggttc agggaatgct ctggacgacc
     2701 agcccggcct gcacggaact cgagacccac gtgctcgatt gggtggtcga catcctgggc
     2761 ctgcccgccc atttcaagtc cgatgccgcc ggcggcgggg tgatccagga ctccgcttcg
     2821 agcgcggccg tctgcgctct gctggccgct cgcgagcgcg cgacccaagg cgagagcaac
     2881 cgccgagggt gcgacggacg actcgtcgcc tacggctcga cccagaccca ttcctcgatc
     2941 gagaaagcga tgggcgtcgt ggggatggga cgcgacaatc tgcgcctgat cgacgtggac
     3001 gactccttcg caatgcgtcc cgatcttcta ttggcgcaga ttcgacgcga ccgggcggca
     3061 ggcctgacgc cgtgtttcgc ctgcgccaca gtgggcacca cgtcctcgaa cgcggtcgat
     3121 cccctgccgg cgatcgggcg catctgccga gaagaacagc tctggatgca tgtggatgcc
     3181 gcgatggccg ggacggccgc ggtgtgtccc gagtaccgtt tccttcagga cggcctcgat
     3241 gcggccgaca gctactgttt caatccgcac aagtggatgt tcaccaactt cgattgcgac
     3301 tgctttttcg tcgccgaccg cgccgcgctg atccgcgcgc tgtcgatcct tcccgagtac
     3361 ctgcgcaacc aggcgaccca aagcggcgcg gtgatcgatt accgcgactg gcacgtgccg
     3421 ctcggacggc gcttccgggc gctcaagctg tggttcgtga ttcgccatta cggggtcgag
     3481 ggcctgcgcc atcatgtccg ccgccacatc gagttggcgc acgcgttcgc ccgctgggtc
     3541 cgggccgacg accgattcga gttggccgtt cccccccctt tgaacctggt ctgtttccgg
     3601 catcgcggag gcgacgatgt caatcagcgg ctcatggacc gtttgaacgc aacaggtcgg
     3661 ctctatctga cgcacaccag gctgggtggg cgcctgactc tccggctttg cgtgggacag
     3721 acctgcaccg aggaacgcca cgtccgcgcc gcatgggacc tgatccggca gagtgttgag
     3781 tcttgagcgt tgagtcgctt cttgagtaca aggccgcaaa catccgacct aacccctgtt
     3841 ccaaggttga acgaacgcac tcagccccac ctattatccg aatgccgcga tatttctctt
     3901 agagtccgcg ttcccttgtt tcgccgagcc ggacactgcc tgccccggcg tgtcgaccgc
     3961 tacaccagcc ggtaccagtt gtcccgctga cgcggctcgt atccgatgtc gcggatcagg
     4021 cggcgcatgt cgtcggcggt catgcggaag gtggcgccgg cctgggccac gacgttctcc
     4081 tcgatcatga tgctgcccag gtcgttggcg ccgaacttga gagcgacctg gccgatttcc
     4141 ggtccctggg tcacccacga actctgcacg ttttcgaaat tgtcgaggta aatgcgggcc
     4201 aaggcttggg tgcgcagata atcgcagctc ccggccatgc gggcttcgag ccgcgtgttc
     4261 tccggttgaa aggtccatcc gatgaacgcc gtaaacccgc cggtcgcgtc ctgctggcga
     4321 cgcagccgat cgagatgttc gatccggtcc tcgatcgtct cgacgtgtcc gaacatcatc
     4381 gtggcgctcg atcgcagccc cagccgatgc gccgtgtcca tcacctcgag ccactcgtcc
     4441 gtcatcgcct tgcgcggcga aacggcccga cgcacccggt ccacaagaat ctccgcaccg
     4501 ccgcccggaa tcgagccgag cccggccgcc cggaattgcc ggaggatttc gtcgagcggc
     4561 agttgaaaca ccttgcggaa atgaacgaac tcgctcgggc tgaagccgtg aatgttgatc
     4621 cgcgggaacc tcgccttgat ccgtgccatg agatcaaggt accaggaaag cggcagggtc
     4681 ggatgatgcc cgccctgcaa gagaatctgc gtcccgccca gggccgccgt ttcctcgatc
     4741 ttccgatcca tctcctccgg ggtgatcaca taggcatccg gttgcccttc ccggcggaag
     4801 aatgcgcaga atttgcagcg gacgtcgcag atgttggtgt agttgatgtt gcgatcgaca
     4861 atgtaggtca cgatctcgtt gccgcgcccg tcataggcgc cggccttcgc gagttcgcgt
     4921 ctgcgattcg ccaccaaccc cagttcagcc agcggcaatc ggtagagacg cagggcctcg
     4981 gcagcatcca cccgccggcc atcccagacc ttctgcagca agccgtcgag cggggcgtcg
     5041 ttgtcgtcca tcagactgga gaatgattcg aacccggcgg ctcggcaagc cccgcgagcc
     5101 ccgactcgag acggtcagcg tcgccatcca gggctcgcgg ggcggctccg gaacaacaat
     5161 tcatgggaga gcattgacat tcaacggtcg aatgcgcctt ggacccatga accggggaac
     5221 gttcaacgcc caacttccaa cgctcaacgt tcaacgaagc ggttcgttaa aagttggatg
     5281 ttgaacgttg cgcgttggac gttcgggtta ccccttttgt taattatcaa cggcttgcgc
     5341 aggcggttca tggaaagcat tgacattcaa cggtttaatg cgcacgggga gcatgaaccg
     5401 atttgtagcc gccgtcgtga gacggcgctg attcctgctt ttgacggagc gagtcagcgc
     5461 ggactcccgt ccgcggctac gaaacggccg cgggacggc
//
LOCUS       DAOQRO010000246         5494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15229_length_5494_cov_5.254642, whole genome shotgun sequence.
ACCESSION   DAOQRO010000246 DAOQRO010000000
VERSION     DAOQRO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15229_length_5494_cov_5.254642"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKK20_09545"
     CDS             <1..576
                     /locus_tag="PKK20_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00170.1"
                     /translation="EPDPDGNLLLNPGFEETRQGRTGGPGAAGVLAEWHYQFAGATPS
                     YVWQELDYQQHPDWGLPEFHTGQGALRTHTDKAGLIHVHQDVETAPNTEYAASVWVRA
                     ADLRGKGFGVAPEDSAGLVLIELDLEGKVLREHPKVELKQAGPYAQLSRTIGTLPDTA
                     QIRFQLETRIHCPYHEGHVTYDDCSLSRVRR"
     gene            complement(871..4005)
                     /locus_tag="PKK20_09550"
     CDS             complement(871..4005)
                     /locus_tag="PKK20_09550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNV00171.1"
                     /translation="MSEPTDSAHCERCGHPLDAELGGVCPVCALDDAAAAPASPEEDF
                     GAAQLMLDDVPLEGATHARLGNYELREPLGAGGMGVVYKAWQTDLERWVAIKLLPIGS
                     LGMPEARERFRQEALTLAKLRHPNIVSIHDFSERDGQAYLVMEYVEGANLEGRSSECL
                     EAPEKAAACVKAAAEGMQAAHEAGVLHRDLKPSNLLLGADGRVRITDFGTARPIAGQS
                     DLTGPGVLLGTLAYLAPEQLKGGAQTASVRSDVYSLGAVLYRLLTGRAPFVAASPAGV
                     LQQVTRADPPPLTLLNPLVPRDLALICSKCLRKDPRDRYGSARELAEDLGRFLANREI
                     KARPLSCIKRMRRWGQLNPGPATAVLLGAVVVATLGVIGALTLEKQVAAVRARIDAAR
                     ELAHREELRAYAQQLDEARSAFAANDPDRAQKALDATRTELRGWEYRFLDRPHTGGHR
                     SWQAHEQGVSCLAANADGTRIVSGDWGGNLRVWDADAGRVLLRRRLHPDRIRSVQFSP
                     DGRQLVTGSEVGTICVVDVETGMVLEWIGTQARLNQAAFFGKAGDVLTVNDRGRLELW
                     CAGNRSPPTVVMWRLPGLLALDIHARSEAYACGDAKGRIHVGLVAKETETSADPPEPE
                     SSPIAKRTWQAHAAEVMSVAWSPDGAHLASASADGQVRLWNPASGDLARSLRERGGGV
                     RCLAWSADGRSLAAATDDGVLLHWRLEGSAEAQVLSGHQGPIFAVAFLGQQHRLVSGG
                     SDRHLRVWDPSSIAVGETFRKVHASGTIRVRFAHRESWFVAAGPEGELWRQDAETGET
                     NWTVQSTRGRMGGLALTTDDAFVVSGGRAGRIEVRSSTNGALVREFTVGTFPIRALDE
                     HPGGRLVAAGGDDRVVQLLDVESGTVSVQFPVQTARIGRIAFSPDGEYVLSCGPDQRL
                     VAWPIRVTNALAATRVPTAVMDFDVGSGGIVTTIGRQEGAQTWLLPGMKPIARMENIE
                     TGFRAVVFAHEGQRLLASDDSGVLRCWDARTGIQLLQLPLPLPGRAVRDLQMSPNDGR
                     LALVGSGGELYLLP"
     gene            complement(4291..5034)
                     /locus_tag="PKK20_09555"
     CDS             complement(4291..5034)
                     /locus_tag="PKK20_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV00172.1"
                     /translation="MISQGQCGVGWPRRGGVAFPVTLWTMVLSARDQSMPALTRLCET
                     YQGPIKAFFRGAGCSRDYVDDLAQDFLVHILHPDVLKTVDPDRGRFRSFLLVCLRRYW
                     SQVQAHGRTIKKGGGVIHVPLDTGSDRPEFDVAHTGPRPDQAFDVEWGRTVVQRAVRR
                     LEAEAADPVRQRLVSHLLPVLYAKERIETQAELARQLGMTPGAVRIAIHRIRKRLRQL
                     IIEELAATVGAGADLEKELRELRAILAGN"
     gene            complement(5043..5447)
                     /locus_tag="PKK20_09560"
     CDS             complement(5043..5447)
                     /locus_tag="PKK20_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00173.1"
                     /translation="MNHRDAEDTEKAIARQSVFRPESVRDGLHTDSDSTFDRMVQASE
                     ERDFGGTLARVPCLDHLLALKLHILKQAWPRRTSKDADDVEMLSRRNGLDLKDARRRK
                     LKERVFEATGPCLQAGVATPMLWRCHFRRAEA"
BASE COUNT          978 a         1906 c         1683 g          927 t
ORIGIN      
        1 gagcccgatc cggatggcaa cctgctgttg aatcccggat tcgaggagac cagacaggga
       61 agaaccggcg gccccggcgc ggcgggtgtt ttggcggaat ggcactatca gttcgccggc
      121 gccaccccaa gctacgtctg gcaggaactg gattaccagc agcatccgga ttggggcttg
      181 cccgaattcc acaccggaca gggcgctctg cgcacgcata cggacaaggc gggcttgatc
      241 cacgtgcacc aggatgtcga gacggctccc aacaccgagt acgccgcatc tgtttgggtg
      301 cgcgccgccg atttgcgcgg gaagggcttt ggcgtcgcgc ccgaggactc agcgggactt
      361 gtgctgatcg agttggacct cgaggggaag gtgttgcgag agcatcccaa ggtcgagctg
      421 aagcaggccg gtccgtatgc gcaattgtcc cggacgatcg gcacattacc tgacaccgca
      481 cagattcggt tccagctcga aacccgcatc cattgtccgt accacgaagg ccacgtgacc
      541 tacgacgatt gctcgctgag ccgagtgcgg cggtgaccct tgcacgaaga gggggggaat
      601 cggagggacg tgttcgaggg cgacgaggcc ctgcctgccc tgcctgccct gcctgccggc
      661 aggcagggca ggcaggcggc aggcaggcgt cgccgctcca agatgcatgt tcgcgcaggc
      721 agtggcgggt tgaccgccgc ttttcgaaga caatgatcgg tcacgccccg cgcaaaacgg
      781 tgctgaatca cacgctccag acgtcctgcg ttgtcggaaa cgcttgcgcc catgagccaa
      841 gaactttcta cgctcaagca gcaaccaaca tcaaggcaac aggtacaact ctccgccgga
      901 ccccaccaag gccaaccgcc cgtcattcgg gctcatctgc agatcgcgca cggcccgccc
      961 aggcagcggc agcgggagtt gcaggagttg aattccagtc cgcgcatccc agcagcgcaa
     1021 cacgccggag tcatccgacg cgagcaaccg ctgcccctca tgcgcgaaga cgaccgcccg
     1081 aaagcccgtc tcaatattct ccatcctggc gatcggcttc atccccggca gcagccaagt
     1141 ctgggcgccc tcctgtcggc caattgtcgt cactattcct ccgctgccga catcgaagtc
     1201 catgaccgcg gttggcacgc gcgtggcggc cagcgcgttc gtgacgcgaa tcggccatgc
     1261 caccaaccgc tggtcggggc cgcagctcag cacgtattcg ccatccggac tgaacgcgat
     1321 gcgcccgatc cgggcagtct gaactggaaa ctggacggag accgtgcccg attcgacgtc
     1381 gagcaactgc acgacacgat cgtcgcctcc ggcggccacc aggcggccgc cgggatgttc
     1441 gtccagcgcg cggatcggaa aggtgccgac cgtgaattcc cggaccagag cgccgttggt
     1501 gctggagcgg acttcgatcc gtcccgcccg tccgcccgaa acgacaaacg cgtcgtccgt
     1561 cgtgagggcg agtccgccca tccgtccccg ggtgctttgg acagtccagt tcgtttctcc
     1621 ggtctccgca tcctgtcgcc acaactcgcc ttctggcccc gctgcaacga accacgattc
     1681 acggtgagcg aagcgcacgc gaatcgtgcc ggaagcgtgg acctttcgga aggtttcgcc
     1741 aaccgctatg gacgatgggt cccacacacg caggtgccgg tcgcttccgc cgctgaccag
     1801 gcgatgctgc tgacccagaa acgcgaccgc aaagatcggc ccttggtggc cagataagac
     1861 ctgcgcttcc gcggaacctt cgagccgcca gtgcaatagg accccgtcat ccgttgccgc
     1921 cgccagcgat cgtccatccg cactccaggc cagacagcgg acgcccccgc ccctctcgcg
     1981 cagcgaacgc gccagatcgc ctgaggcggg attccacaat cggacctggc catcggctga
     2041 cgcgctggcg aggtgggcgc cgtccggact ccatgccacg ctcatcacct cggcggcatg
     2101 ggcttgccag gtgcgtttgg cgattgggct cgactccggc tcgggtgggt cggcggaggt
     2161 ctcggtttcc tttgcgacca aaccgacgtg gatgcgtccc tttgcgtcgc cgcaggcata
     2221 agcctccgac cgggcgtgga tatccagcgc caggagcccg ggcaacctcc acatcaccac
     2281 tgtcggcggg cttcgattgc ccgcgcacca cagttcgagt cgaccgcggt cgttgacggt
     2341 caacacatcg cctgccttgc cgaaaaaggc cgcctgattt aggcgggctt gggtcccgat
     2401 ccactcgagc accatgcccg tctcgacatc gaccacgcag atcgtgccga cttcgctgcc
     2461 ggtcaccaac tgccgaccat ctgggctgaa ttgcaccgaa cggattcgat cgggatgcag
     2521 gcgccggcgc agcaacaccc gccctgcgtc agcatcccag acgcgcaggt tcccgcccca
     2581 atcgccgctg acgatccgag tcccatcggc gttggcggcg aggcaggaca cgccctgttc
     2641 gtgcgcctgc caggaccgat gccctccggt gtggggacga tccaggaacc gatactccca
     2701 gccccgcagc tccgtccgag tggcatcgag cgccttctgg gctcggtcag gatcgttcgc
     2761 cgcgaaggcg ctccgcgcct cgtcgagttg ctgggcgtaa gcgcgcaact cttcgcggtg
     2821 tgcgagttcc cgggccgcgt cgatccgggc ccgaacggcg gcgacttgtt tttcgagcgt
     2881 caacgctccg atcaccccca gcgtcgccac caccaccgca cccaggagca cggccgtcgc
     2941 cgggccggga ttcaactgcc cccagcgccg catccgcttg atgcaggaaa gggggcgcgc
     3001 cttgatttca cgattggcca ggaaccgccc caaatcctca gccagttccc gtgccgatcc
     3061 atagcggtcg cgcgggtctt tgcgcaggca tttcgagcag atgagtgcca ggtcgcgcgg
     3121 gaccagtgga ttcagcaagg tcagcggcgg tggatcggct cgtgtcacct gttgcagcac
     3181 gcctgccggt gaagccgcca cgaacggcgc gcgtccggtc aacaagcggt acagcaccgc
     3241 ccccaacgag tagacgtcgc tacgcacgct cgcagtctgc gctccgccct tgagttgctc
     3301 gggtgccagg taagccagcg tgcccagaag cacgccgggg ccggtcaggt ccgattgacc
     3361 cgcaatgggg cgtgccgtgc cgaagtcggt aatgcgcacc cgaccgtcgg cgccgagcag
     3421 cagattcgag ggcttgagat cccgatggag cacgcctgcc tcgtgggcgg cttgcatccc
     3481 ctcggcggcc gccttgacgc aagccgccgc tttctccggc gcctcgaggc actcgctcga
     3541 ccggccttcg agattcgcgc cctcgacata ctccatgacc aggtacgcct gcccgtcgcg
     3601 ttcgctgaaa tcatggatgc tcacaatgtt cgggtgccgg agtttggcga gcgtgagcgc
     3661 ctcttgtcgg aaccgttcac gcgcctccgg catgcccaaa gagccgatcg gcagaagctt
     3721 gatggcgacc cagcgttcga ggtcggtctg ccaggcctta tagaccacgc ccatgccgcc
     3781 ggcccccaat ggttcgcgca gttcgtaatt gcccagccgc gcgtgcgtcg cgccttcaag
     3841 aggcacatca tcgagcatga gttgggcagc cccgaaatct tcctccgggc tggcgggggc
     3901 ggcggctgca tcgtccagtg cgcacacggg acagaccccg cccaactccg catcgagcgg
     3961 atgtccgcat ctttcgcagt gggcactgtc agtcggttcg ctcatcggcg cagtctcccg
     4021 gggccgtcga ccctcgcccc agcccggcgc acccgcgcag cggactccgg ctggggagag
     4081 cacccgcgtg aggggctggg tctaacccga aatcaagcat cggactcgcg tcgagatcgt
     4141 ctgccccccc gcaactcttc accgtcctcc atcgccatga ctccgcggtg tccatgccaa
     4201 gagaaaacgg aaatctggcg gaatgttacc gagccgcccg ccgttccggc cagtcggttc
     4261 tttgttcctt gcgttctttt gcggcttccc ctagttcccc gccaggatcg cccgcagctc
     4321 ccgcaactcc ttttcgaggt ccgccccggc ccccaccgtg gcggccagtt cctcgatgat
     4381 caactggcgc agacgcttcc gaatccggtg gatggcgatg cgcaccgcgc caggtgtcat
     4441 cccgagttgg cgcgcgagtt cagcctgggt ctcgatccgc tccttcgcgt acagcacggg
     4501 caacaaatgg gagacgagcc tctgccggac cggatcagcc gcctccgcct cgagccggcg
     4561 gaccgcgcgc tgcaccaccg tgcgccccca ttcgacgtcg aacgcctgat ccggtcgcgg
     4621 accggtgtga gcaacatcga attcgggccg gtccgaacca gtatccaagg gcacgtggat
     4681 gaccccaccg cctttcttga tcgtgcggcc gtgcgcctgg acctggctcc agtaacgcct
     4741 caggcagacc aacaagaacg accggaaccg ccctctgtcg ggatcgactg tcttgagcac
     4801 gtcgggatgc aggatgtgga ccagaaaatc ctgtgccagg tcgtccacat aatctcgcga
     4861 gcagcccgcg ccgcggaaga aggccttgat gggcccttga tacgtctcgc agagccgggt
     4921 caatgcgggc atcgactgat cccgcgccga cagcaccatt gtccagagcg tgaccgggaa
     4981 agcgaccccg ccccgtcgag gccacccgac gccacactgt ccttgggaaa tcactgtgcg
     5041 acttatgcct cggcacgacg gaaatgacaa cgccaaagca tcggagtggc gacgcctgcc
     5101 tgcaggcagg gccccgtcgc ctcgaacacc ctttctttca acttgcggcg ccgagcatcc
     5161 ttcaaatcca gcccgttgcg tcggctcagc atctcgacat catcggcatc cttggacgtg
     5221 cgacgcggcc acgcctgttt gagaatgtgc agcttcaacg ccagcaagtg atccaggcaa
     5281 gggacgcgcg ccagggtgcc gccaaagtca cgctcctcgg aggcctggac catacgatcg
     5341 aaagtcgagt ctgaatcggt gtggagtccg tccctcacag attcggggcg gaacacagat
     5401 tgccgggcga tagctttctc cgtgtcctcg gcgtctctat ggttcattcc gcacggtgac
     5461 attcagcgag ggtgatcaac cacagaggca caga
//
LOCUS       DAOQRO010000247         5482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15271_length_5482_cov_5.284503, whole genome shotgun sequence.
ACCESSION   DAOQRO010000247 DAOQRO010000000
VERSION     DAOQRO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15271_length_5482_cov_5.284503"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..1330
                     /locus_tag="PKK20_09565"
     CDS             557..1330
                     /locus_tag="PKK20_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00174.1"
                     /translation="MSTIRVSRTPPPMQTEWIEEKQLAQTLRRHEAFWRGELETGPLM
                     WVTVPGARPSRPVPEPASEAQLWTDVDYVIAAAESALAGTHYAGDALPVFCPWLGPNQ
                     FAAWLGADLVLCPRQSTSWSKPFVQDWGNHADLRIDPGNRWWQLYLQLVRESVRAGRG
                     KWVTGYPDLHSGIDALSALRGPEQLALDLKLAPAAIHRAMRQMTALWKEVIDTVSALV
                     LPAGQGTSNWTMGWSQDRFLCIGQNDFSCMIGPKKPTPP"
     gene            1469..2857
                     /locus_tag="PKK20_09570"
     CDS             1469..2857
                     /locus_tag="PKK20_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00175.1"
                     /translation="MKAHRTRRQFLARTATSAFGFFLSTRHGFATAAAANEELNLALV
                     GVGGRGEWFLGALPGMRTRFAALCDTHAYRLNRAGEKFPEAKRYRDFRRMLEESADRL
                     DGVVVATPDHSHAVISARAMRAGKPVFCEKPLTRTISETRALRELAASTQVATQMGNQ
                     GTSSDPFRRSVEIVQSGVLGEIREVLTWNESGGGGPHPRPTTSEAVPEDLDWDVWLGP
                     AAARPYHREWANWHRWRDFATGQLGNWAVHSANLAFKAFRLDEFWKGTSAELPEGCHF
                     RVAAKVSEVLADTFPTWELIEFSAPRRRNQPAFAMIWCNGRAPGGRERIEERLGRKLD
                     WGDAGERKWNDHAGLLIVGTRGMLHSTGHNMTFSLLPEADFQDLLGPPRSLPRSPGHE
                     REWLDAIRGGASACSNFADYGSLLTEWLLLGNVATQTEGAIEYDPRAGRVVNNESANR
                     LCKALYREGWTL"
     gene            2870..3874
                     /locus_tag="PKK20_09575"
     CDS             2870..3874
                     /locus_tag="PKK20_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNV00176.1"
                     /translation="MRPQLPWVLSLIVGLACLSVAAGDAIPEHQRKQIWDAAPASPRV
                     APKQPRRVLIWNTPAHLMEKDPHKGYCIPYGAVALEAIGRKSGAFEPVISDDLSAFLP
                     GNLRAFGAVVLNNSSGAWITPTPANLTTDAFRALGADAESVERALRRSFLDYVANGGG
                     VVALHFAIAGNAHWPEFQQLIGARFTGHPWNEEIGVTVEEPSHPLVAAFGGKSFRIAD
                     EIYEYGPPYDRAKLRVLLSLDPARSNMGVKWINRTDNDFALAWVKAHGKGRVFNTSFG
                     HRTELFWEPRILAFYLDAIQFATGDLEAPTTPRTDRLIRSVPGSEPVPGRPGFREPFQ
                     "
     gene            complement(3897..4115)
                     /locus_tag="PKK20_09580"
     CDS             complement(3897..4115)
                     /locus_tag="PKK20_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00177.1"
                     /translation="MREHHAGELRRESAELRAEGIVAKELRRETALTVGWTARGLELR
                     TLKSAGVELHRWTKGTQERCVGDGKTTV"
     gene            4208..4465
                     /locus_tag="PKK20_09585"
     CDS             4208..4465
                     /locus_tag="PKK20_09585"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HNV00178.1"
                     /translation="MAKQTFRFKSPGALSVLLVGDFTHWQEKPIAMKKSGHGIWRASV
                     DLPPGVYHYRFMVDGEWRDDPESTLRVSNPFGSENAVRKVT"
     gene            complement(4762..>5482)
                     /locus_tag="PKK20_09590"
     CDS             complement(4762..>5482)
                     /locus_tag="PKK20_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNV00179.1"
                     /translation="NMDPRHRDCLAFVRICSGRFERDMTVIHTRTGKRVRLACSHKLF
                     GRKRETVDVAYAGDVVGLVGHSEFRIGDTLAENSALRYDEIPRFAPESFAWLHSASTA
                     QFKRFREGLAQLLEEGVVQSFRLPNATQRVTLIGAVGPLQFEVVQYRLQTEYGAESRL
                     EPAPWQLLRWVVDGMVGEPTLPSGARLAIDVAGQTVILFTEEWSCNFFAERNPGVKLA
                     VLPPRIPVDGPEAALSASGHG"
BASE COUNT         1015 a         1767 c         1733 g          967 t
ORIGIN      
        1 gatttacccc agtggccgtt tcgagtccag caaaaaaatg tccaaactcc aggtcgcgga
       61 caggagtccg cgctgactcg ctccggcgaa cgcagacacc agcgccatct cacgacggcg
      121 gctacggagg cgtagccgtc ccgcggccgc cgcgtagccg cggacgtcag tccgcgctga
      181 ctcgctccgg cgaatacaga catcagcgcc gtctgacgac ggcggctacg gaggcgcgtc
      241 gggctcccag aacaacgtcg aaaacggcga cgatgcgatc ctggcgacgg accagacggc
      301 ggcgagcggg aaacacagcc tgaggattgc agctcgccat ccagcccttc ctggggaaat
      361 aacaccactc ggagtcttaa gacaggctct gaggggggga aaggcatgac tggcagggag
      421 ctttgtgctg taagcttttg gcgatcagcg acaaatcttg catccgtgag gatcgcaact
      481 tagactccgg tattccaccg tgttcttggg ccgtgattga gcgaaaagag tacctggaac
      541 tgctgacgac acggttttga gcacaatccg tgtttcccgc acgccgccgc ccatgcaaac
      601 cgagtggatt gaggagaagc aactggccca gacactccgg cgccatgaag cgttctggcg
      661 cggcgaactc gaaaccggcc cgctgatgtg ggtgaccgtg cccggcgccc gaccaagccg
      721 accggttccc gaaccggcgt ccgaagccca gttgtggacc gacgtggatt acgtgatcgc
      781 agccgcggaa agcgcgttgg cgggaaccca ctacgccggc gacgcgctgc cggtgttttg
      841 cccctggctg ggcccgaatc agttcgccgc ctggctgggg gcggacctgg tgctttgccc
      901 ccgacaatcc acctcgtgga gcaagccttt cgtgcaggat tgggggaacc atgccgacct
      961 gcgtatcgac ccgggcaacc gctggtggca gctctacctt cagctcgtgc gggagtcggt
     1021 tcgggcaggc cgcggaaaat gggtcaccgg ctatcccgat cttcattcgg gcatcgatgc
     1081 gctgagcgcg ctccgcggcc cggagcaact cgcgctggac ttgaagctcg ccccggctgc
     1141 gatccaccgc gccatgcggc agatgaccgc cctctggaaa gaagtgattg ataccgtgtc
     1201 agccctggtc ctgcctgccg ggcaagggac ctcgaattgg acaatggggt ggagccagga
     1261 tcgcttcctt tgcatcggtc aaaacgactt ttcctgcatg atcggtccga aaaagccaac
     1321 gcctccctga ccaacgcagt tcaccttgag cgatggccgc ggtctgcttc gacaaccaaa
     1381 actccaggcc cccactcggg ccagcgccga gccggcataa tagagttgtc caccgcccgg
     1441 ttatgaccca cagtgccggt cagaaagcat gaaggctcac cgcacccgcc ggcagtttct
     1501 ggcccgcacc gccaccagcg cgtttgggtt cttcctcagc acacgccatg gcttcgcgac
     1561 agccgcggcc gccaacgagg aattgaacct cgcactggtt ggtgtgggtg ggcgtggcga
     1621 gtggtttctc ggcgccctgc cgggcatgcg gacgcgcttt gccgccttgt gcgacacgca
     1681 cgcctatcgg ctgaaccgcg cgggcgagaa gttccctgag gcgaaacgct accgggactt
     1741 ccggcggatg cttgaggaat cggccgatcg actcgacggc gtggtggtcg cgacgcccga
     1801 tcacagccat gcggtgatca gcgcccgagc tatgcgcgcc ggcaaaccgg tgttctgcga
     1861 gaaaccgctg acccgtacca tcagcgagac ccgcgcgctc cgcgaacttg cggcgtccac
     1921 gcaagttgcc acgcagatgg ggaaccaggg cacgtccagc gacccgtttc gccgttcggt
     1981 ggaaatcgtc cagtcgggcg tgctgggaga aattcgtgaa gtgctgactt ggaacgagtc
     2041 cggcggcggc ggaccgcatc cgcgcccgac caccagcgaa gccgtcccgg aggacctcga
     2101 ctgggacgtg tggctgggcc cggccgcggc gcgtccgtac caccgcgaat gggcaaactg
     2161 gcatcggtgg cgcgacttcg ccaccggcca gctcggcaac tgggctgtgc actcagccaa
     2221 cctggcgttc aaggcgtttc gtctcgacga attctggaaa ggcacctcgg cggagctgcc
     2281 cgagggatgc cacttccgcg ttgcggcaaa ggtgtccgag gttttggccg acacgttccc
     2341 gacgtgggag ctcatcgagt tttcggcgcc ccggcgcaga aatcagccgg cgttcgccat
     2401 gatctggtgc aacggccgcg cgcccggcgg acgcgagcgc atcgaggaac gcctcggacg
     2461 gaagctggac tggggggacg ccggggagag aaagtggaac gatcacgcgg gcctgctgat
     2521 cgtggggacg cgcgggatgc tccattccac cgggcacaac atgacgttct cgcttttgcc
     2581 ggaagccgac ttccaggatc tgctcggacc gccacgcagc ctgccgcggt cgcccggaca
     2641 cgaacgggaa tggctcgacg ccattcgcgg cggggcgtcg gcgtgctcga atttcgcgga
     2701 ctacggttcg ctgctgaccg agtggctgct cctcgggaac gtagccacac agaccgaggg
     2761 tgcgatcgag tacgatccgc gcgctggccg cgtcgtgaac aacgagtccg ccaatcggct
     2821 ctgcaaagcc ctctatcgcg aaggctggac cctctgaagg aggccgatca tgcgccctca
     2881 actcccctgg gtcctatccc tcatcgtggg gttggcctgc ctttctgtcg cggcgggcga
     2941 cgccatcccg gagcaccagc gcaaacaaat ctgggatgcg gcgcctgctt cgccccgggt
     3001 agcgccgaag cagccccgcc gcgtcctgat ctggaacacg cctgcgcact tgatggagaa
     3061 agacccgcac aagggttact gcatcccgta cggagccgtc gccttggagg cgatcggtcg
     3121 caagagcggt gccttcgagc cggtgatcag cgatgacctg agcgcctttc tgccggggaa
     3181 cctccgggcc ttcggtgcgg tggtcctgaa caacagctcg ggtgcctgga tcactcccac
     3241 tcccgccaac ctcaccacgg atgccttccg cgcattgggc gccgatgcgg agtccgtcga
     3301 gcgagcgctt cgccgcagct tcctggacta cgtcgcgaac ggcggtgggg tggtggcgct
     3361 tcatttcgcc atcgcaggga acgcgcactg gccggagttc cagcagttga tcggcgccag
     3421 gttcaccggc catccttgga acgaagagat cggcgtgacc gtcgaggagc catcacatcc
     3481 actagtggcc gcgtttggcg gaaaaagctt ccggatcgcc gatgagatat acgagtacgg
     3541 tccgccgtac gaccgcgcga aactgcgggt cctgctctcg ctcgatccgg cccgttcgaa
     3601 catgggcgtg aagtggatca accggacaga caacgatttc gccctcgcct gggtgaaggc
     3661 gcacggcaag ggacgcgtct tcaacacctc gttcggtcac cgcaccgagt tgttctggga
     3721 gcctcgaatt ctggcgttct acctggatgc catccagttc gccaccggcg acctcgaggc
     3781 gcccacgacg ccgcggaccg accggctgat acggtccgtg cccggttcgg aaccggttcc
     3841 cggacggccg ggttttcgtg agccttttca atgaacacga ttcatcgaca ctgtggtcaa
     3901 accgtagtct tgccatcgcc aacgcatcgc tcctgagtac ccttggtcca ccgatgcagt
     3961 tcgacccccg cgctcttcag cgtcctcagt tccagtccgc gcgcggtcca tcccaccgtc
     4021 aacgccgtct cccgcctcag ctccttcgcg acgatcccct cggcccgcaa ctccgcgctc
     4081 tcccgccgca actccccagc atggtgctcc cgcatatggt gtgaaacctg ttttgggttc
     4141 tttgtgtggg tttctgtgcc ggactttctt gcggacccgc ccgactgtgc tactcttgcc
     4201 cgatactatg gcaaagcaaa cgtttcgttt taagagtccc ggcgcgctga gtgtcctgtt
     4261 ggttggagac tttacccatt ggcaagagaa gccgatcgca atgaagaaat cgggtcatgg
     4321 gatttggagg gcgagcgtgg acctgccacc gggggtttat cattaccgtt tcatggtcga
     4381 tggagaatgg cgggacgatc ccgaaagcac gctccgggtc tccaacccgt tcgggtcgga
     4441 aaacgccgtt cggaaagtca cctgaccccg ggtgcaatcc ccagcccgga cgtttcacac
     4501 cggtgcgcgt gatcggatat ccgggctttc gaaaccgcct tgaatccttg accggatgaa
     4561 acacgttgca gacaagtcaa gtttccgtgc tgcgttaaac gagtcaagga ttctaatgcg
     4621 cgaggcgcgg cagagaagga cggtgaaaca cggggacgcc tcatggggag gactggattc
     4681 ggtctgatgc gctcctctcc agacatcata cgagagcgga ccccttgtgc attgacgggg
     4741 caggaaatag gcgcgttagg ctcaaccgtg gcctgacgcg ctcaaagccg cctccggccc
     4801 gtcgacagga atcctgggag gcagaacggc cagcttcacg ccgggattgc gctcggcgaa
     4861 gaaattgcag gaccactcct cggtgaataa gatcacggtc tgaccggcga catcgatcgc
     4921 caggcgcgct ccagagggta gcgtcggctc gccaaccatg ccgtccacca cccaccgaag
     4981 caactgccac ggcgcgggtt cgagtcggga ctcggcgccg tactcggtct gcaagcggta
     5041 ctggaccacc tcgaattgga gggggccgac tgcgccgatc agggtaacgc gctgggtggc
     5101 attgggcagg cggaacgact gcaccacccc ttcctcgagt agttgggcca agccctcgcg
     5161 aaagcgcttg aactgcgccg tgctcgcgct gtgcaaccac gcaaaactct ccggagcgaa
     5221 ccgagggatt tcgtcgtacc gcaatgccga gttctccgca agcgtgtcgc cgatccggaa
     5281 ctcggaatgg ccgaccaatc cgaccacgtc gcctgcgtac gcgacgtcca cggtctcgcg
     5341 cttgcgtccg aaaagcttgt gcgaacaggc cagccgcacc cgtttgcccg tgcgggtgtg
     5401 aatgactgtc atgtcgcgct cgaatcgacc ggagcagatg cggacgaagg cgaggcagtc
     5461 gcgatggcgc gggtccatgt tg
//
LOCUS       DAOQRO010000248         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15348_length_5462_cov_4.793971, whole genome shotgun sequence.
ACCESSION   DAOQRO010000248 DAOQRO010000000
VERSION     DAOQRO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15348_length_5462_cov_4.793971"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..4149
                     /locus_tag="PKK20_09595"
     CDS             250..4149
                     /locus_tag="PKK20_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00180.1"
                     /translation="MKPILPITLICLGLLAPRCGASEPDSSLSLWLPLDEGLGSLAID
                     RSPNQLEAELANVRWAKGAFGTAAHFGGTNAFIELPPVPSLNGATQCTISVWATWEGT
                     GRYPNLLTTHTWSPGGMMLFVSDNSCSFRMGRPGHRAGVPGNAWDEIGVGLLNALPMR
                     QWVHLCVVFALPNITTYVNGKPVDQGDWPYPVSADGLRLGGWSSPVSHHGLISDLRIY
                     GRALTGDEVSDLAENSTRTNAEYTLVDESAYAPPLAARFENRHATLAIDQQGRICSLR
                     SKASGRELLACRQELVSARLKDGRQITARKASFANNTLTFEFSPRRGVAVLAVDERED
                     FFTFTIQSVTVPDVASLTFFNLRVTPAKYRGNMVNMLSDDTDAVCLRGYDLPVEMALD
                     GNLPGLRVWTVAERGLTGWRAGLAAGPKPEMTAMLRAMTMEAGVPVSKLGGAWALGAE
                     ANRGSYLFADLSLASVDDWIDIARRGGFTHIHLHGWWDTLGHYGVNTNLFPNGLADMK
                     EAVARIHAAGLKAGIHTLTACIDPRDAWVTPEASPHLIATDSYTLARPMTPTDTAIYV
                     NEKPSPNHDVVFTYMGNGNAIRIGTEIVQYGEVVSQPPYAFAKCQRGAFKTVPAAHAA
                     GDPVDYLQQRYIAFYPEPGSPLAYELADHIANVFNTCQLDQIYFDGSEGMMSRYGIDF
                     MRRTIFERLKGEVLAEASEWGAHSWWFHSRLGAWDHPVWAVKRFHDLHIASDAQFRAS
                     ELIEPQMGWWAPREPSAHARGHFLDEMEYFAAKNLGLDSAMSIQGVNVSRHPLSFHVE
                     NQFTVLGWHEQLRLARYFDSQTIARVAVPGDEFRLRQDPGGAWKFTPVKMSAHRISAL
                     GNGSERWTSANPFADQPVAARIEALYSVAAYDSPKRICVADFADLEAFQQATSSSSVL
                     LQLRAVADDTRGGERNLSLRARNQGTARRGAWARAGLAFPAPYRNLSGAEALGVWVKG
                     DGKGALLNIQLCTPREYTHALSDHYVLLDFTGWRYVELLLRERDLEQMSDYEWPYGGG
                     YDLYRNPLDMAHVSEVNLYLNNLPPGDSAEVILGPVMALAVQPAELRNPVLTLNGQSL
                     ALPVTLKSGDFLEIDTTGNGTHYDHKGNLLTRVRPMTASGWPVLKSGENAIAFDCEKP
                     TGASARAEVTLTASGQPFGTVNPRARIDWKHLTREYELPRWITAPEGADNIWDVAVRP
                     DEQARLEIELSGGMDTPTLTVNGEVLRFPVVLKPAQRLICRGDRHWQIIDAERAQVTE
                     GDLTVAAPILKSGSNRVSFTCIAPERALVKLVKVYKP"
     gene            complement(4387..4647)
                     /locus_tag="PKK20_09600"
     CDS             complement(4387..4647)
                     /locus_tag="PKK20_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00181.1"
                     /translation="MPCLRRKAQRPVCRVGHGTGRRGRQALHAAHTDAHRRPSDALTF
                     QGRFAELMSPHAPPADQQALARQLLDAMGLTAAVLGGTPFNN"
     gene            4911..5411
                     /locus_tag="PKK20_09605"
     CDS             4911..5411
                     /locus_tag="PKK20_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNV00182.1"
                     /translation="MQDILFYVFAALTLVCGFLVVANPISRSPVTSALFLVLTIVSLS
                     GLFVLLHAFFLAAVQIVVYAGAALVLFLFVIMMLDLKAEERRRITRLGLIGGGLVAVA
                     MAWLFRVVPREAALVASGPAHVEGSTPALGALLFTQYLLPFEAISLVLVAGMVGAILL
                     SRKEPS"
BASE COUNT          945 a         1762 c         1684 g         1071 t
ORIGIN      
        1 cccacaccga ttcagggcgg aacacagatt cccgaagagg acaagttctt cctcacgaat
       61 tggcccaagg attgaatctg gcaaccgtaa cctaggcacc aagtagggcg gaatgtagtt
      121 gaaggcctga cgcaaaacgg ttagttagcc cgcggttggg gcttggtgtc ttttcatggc
      181 ctgccgtgtg gcaggttttc gtcaagcttc caaccgtttt ttttccgatt cctgtgggat
      241 ggctgtggaa tgaaaccgat cctgccgata acgctgattt gcctggggct gctcgccccg
      301 cggtgcggcg catccgaacc tgattcgtcg ctctcgctct ggctgccact ggatgaaggt
      361 cttggcagtc tcgccatcga tcgctcgccg aatcaactcg aggccgaact cgccaacgtg
      421 cgatgggcca agggggcctt tggcacagcc gcccatttcg gcggcaccaa tgcgttcatc
      481 gaacttccgc ccgttccttc cctgaacggc gcaacgcaat gcaccatctc ggtctgggca
      541 acatgggaag ggacggggcg ctatcccaat ctgctgacga cgcacacttg gagtcccggc
      601 ggcatgatgc tttttgtcag cgataactct tgttccttcc ggatggggcg gcccggccat
      661 cgggctggtg ttccgggcaa tgcctgggac gaaattggag ttggattgct gaatgcgctt
      721 cccatgcggc agtgggttca tctgtgcgtg gtctttgcgt tgccgaacat caccacatac
      781 gtcaatggca aaccggttga ccagggggat tggccttatc ctgtctcagc cgacggcttg
      841 cgcctcggag gctggtcgag ccccgtttcc caccacggcc taatcagtga cctgcgcatc
      901 tacggtcgtg cgctcacggg ggacgaggtc agcgacctcg cggagaattc cacgcgcacc
      961 aacgctgagt acactctggt ggatgaatcc gcgtacgccc cgccgctggc ggcgcgtttt
     1021 gagaaccgcc acgccactct ggccatcgac cagcaaggcc gaatctgttc gctgcgcagc
     1081 aaggcatccg gccgcgagtt gttggcctgc cggcaggaac tggtgtcggc ccggctcaaa
     1141 gacggacgcc aaatcaccgc ccgcaaagcc tcgtttgcca acaatacgct cacgtttgag
     1201 ttctcaccga ggcgtggtgt ggcagttctg gccgtggatg agcgcgagga cttctttacc
     1261 ttcacgattc agtccgtgac cgtgcccgat gttgcctctc tgaccttttt caacctgcgg
     1321 gtcacgccgg caaagtatcg cggcaacatg gtcaatatgc tttcggatga cacagatgcc
     1381 gtctgcttgc ggggttacga ccttcccgtc gagatggcac tggacggcaa tctccccgga
     1441 ctgcgagtct ggaccgtggc ggagcgcggc ctcaccggat ggcgggctgg gttggccgcg
     1501 ggtcccaaac cggaaatgac cgcgatgctt cgcgccatga cgatggaggc aggggtgcca
     1561 gtctcgaagc tcggcggcgc atgggcactt ggcgccgagg cgaaccgcgg ctcctacctc
     1621 ttcgccgatc tctcgctggc ttccgtggac gactggattg acatcgcgcg ccgaggcggg
     1681 ttcacgcaca tccacctgca cggctggtgg gacacgctcg ggcactacgg cgtcaacacc
     1741 aacctctttc ccaacggcct tgcggacatg aaagaggccg tcgctcgcat ccatgcggcg
     1801 ggcctcaaag cgggcatcca tacgctcacg gcctgcattg atccgcgaga cgcctgggtg
     1861 acacccgagg ccagcccgca cctgatcgct accgattcct atacgctggc gcggcccatg
     1921 acgcctacgg acaccgcgat ctacgtgaac gagaagccga gcccgaatca cgatgtcgtt
     1981 ttcacctaca tgggcaacgg caatgccatc cgcatcggca cggaaatcgt gcaatacggc
     2041 gaggtggtgt cccaaccgcc ctatgccttc gcaaaatgcc agcggggcgc gttcaagacc
     2101 gtcccggcgg cccacgcggc tggcgaccct gtcgattatc tccagcaacg ctacatcgcg
     2161 ttctatcccg agcccggctc gccactggcc tatgaactgg ccgaccacat cgccaacgtc
     2221 ttcaatacgt gtcaactcga ccaaatctac tttgacggct ccgaaggcat gatgagccgc
     2281 tatggcatcg actttatgcg ccgcacgatt ttcgagcgcc tgaaaggtga ggttttggca
     2341 gaggccagcg aatggggcgc gcacagttgg tggtttcatt cccggctcgg cgcctgggat
     2401 catcccgtct gggccgtcaa gcgatttcat gatcttcaca tcgcctccga cgctcagttc
     2461 cgcgcctccg aactgatcga gccgcagatg ggctggtggg ccccccgcga gccatcggct
     2521 catgcacgcg gtcatttcct ggacgagatg gagtacttcg cggcaaagaa cctgggactc
     2581 gactccgcca tgtccatcca gggcgtcaac gtttcccgcc acccgctgtc atttcatgtc
     2641 gagaaccagt tcaccgttct cggctggcac gagcaactgc ggttggcgcg ctacttcgac
     2701 tctcagacga tcgcacgtgt ggcggtcccc ggcgacgagt tccgtctgcg gcaggacccg
     2761 ggcggcgcgt ggaaattcac gccggtcaaa atgtcggctc atcgcatcag tgcactcggc
     2821 aacggctccg aacgctggac gagcgcgaat ccgttcgccg atcaaccggt cgcggcccgc
     2881 atcgaggcgc tctattccgt ggcggcctac gacagtccca agcgcatttg cgtagcggac
     2941 ttcgccgact tggaggcctt ccagcaggcc accagttcgt cctcggtgct gcttcaactc
     3001 agagcggtgg cggacgatac gcgtggaggc gagcgcaatc tgagcctgcg tgcccggaac
     3061 cagggcacag cacgccgcgg cgcatgggcc cgggcaggtc tcgcctttcc ggccccctac
     3121 cgcaacctct ccggcgccga ggcgctgggc gtgtgggtca agggcgacgg taagggcgcg
     3181 ctgctgaaca tacagttgtg cacgccgcgc gagtacacgc atgcgctttc cgatcactac
     3241 gtgctccttg acttcacggg ctggcgttac gtcgaactcc tgctgcgcga gcgggatctg
     3301 gagcagatgt ccgactatga gtggccgtac gggggcggct acgatctata ccgcaacccg
     3361 ctggacatgg cccacgtttc cgaggtcaac ctctacctca acaacctgcc tcccggcgac
     3421 agcgctgaag tgatcctcgg ccctgtcatg gcgctcgccg ttcagcctgc tgaattgagg
     3481 aatccggtgc tcaccctgaa cggacagagc ctcgccctgc ctgtcacgct gaaatccggc
     3541 gatttcctcg aaatcgatac gaccggcaac ggcacgcact acgaccacaa aggcaatctg
     3601 ctgacgcgag tccgtccgat gaccgcgtcc ggttggccgg tgctgaagtc gggcgaaaac
     3661 gcaatcgcct tcgattgcga gaagccgacc ggcgcgagcg cccgcgctga agtaacgctc
     3721 accgccagcg gccagccgtt tggcacagtc aatccgcgcg ccaggatcga ctggaagcac
     3781 ctgacgcgtg aatacgagtt gccgcgctgg atcaccgcac cggagggcgc ggacaacatc
     3841 tgggacgtgg cggttcgtcc ggacgagcaa gccaggctgg aaatcgaact gagcggcggc
     3901 atggacacac ccaccctgac cgtgaacggc gaagttctcc gcttcccggt tgtgctcaag
     3961 cccgcccagc ggctgatctg ccgcggtgac cgtcattggc agatcattga cgcggaacgc
     4021 gcccaagtta cggaaggcga tctcaccgtc gccgcaccca ttctgaagag cggatcgaac
     4081 cgtgtttcgt tcacctgcat tgctcccgag cgcgcgttgg tgaaactcgt caaggtttac
     4141 aaaccatgat ggccggacac ccccttctgg ctgtcgcttc atccaagtgg cttgattcca
     4201 gcgtttggtg ggccttccaa cgccctgctg tttgctttgc tgcgagaagg acccgtgcac
     4261 gccaatcaca cccgaccctt tcccagtcgg cttcttctgc gctctgcagg ggccgcgcgg
     4321 ccccgtcgcg attccgtgca ctcgatcttg gacccgcgcg gaatcgcagc agacaatggc
     4381 ggcacttcag ttgttgaacg gagttccgcc cagcaccgcc gccgtcagac ccatcgcgtc
     4441 gaggagttgg cgggccagag cctgctggtc cgcgggcggg gcgtggggcg acatcaactc
     4501 cgcgaagcgc ccctggaacg tcagagcatc tgaaggtcgt cggtgggcat cggtatgcgc
     4561 cgcgtggagg gcctgcctgc cgcgcctgcc tgtgccgtgc ccgacacggc agacaggccg
     4621 ctgcgctttg cggcgcagac agggcacgcg acctcgtgtg ctctgttcgc gcgcacgaag
     4681 gcgttggagt ggggacgccc tcgtcccccg gcgatgagag ttgcaggccc gctttggcag
     4741 gcgttacccc tccggcaatc tccatccgtt gcaggcccgg cgccctcacc acgcgacaga
     4801 acaccgagca caaccagatg caccgctcga acgcggcctc gagcgcgggc ttgcgcaaac
     4861 cggccggctc cggttcaatc gcgtcccaac aaacggcggc ccttcaaggc atgcaggata
     4921 ttctgtttta cgtttttgcg gcgctgacgc tggtctgcgg ttttctggtc gtggcgaacc
     4981 cgatcagccg cagcccggtg acgagcgcgc ttttcctggt cttgaccatc gtctcgctgt
     5041 ccggcttgtt cgtcctgctg cacgccttct tcctggcggc ggtgcagatc gtggtctatg
     5101 ccggggcggc gctggtgctg tttctcttcg tcatcatgat gctcgacttg aaggcggagg
     5161 agcgccgtcg aatcacgcgc ttgggcctga tcggaggcgg gttggtcgcc gtggcgatgg
     5221 cgtggctatt ccgcgtggtt ccgcgggaag cggcgcttgt cgcctccgga ccagctcatg
     5281 tcgaggggtc gacgccggct ttgggggcgc tgttgttcac gcagtacctg ctccccttcg
     5341 aggcgatatc gctggtgttg gtggcgggga tggtgggagc gattctgctg agcaggaagg
     5401 agccatcgtg accgtccgcc ttgcaccctt cctgacggtg agcgtggtgc tgttctgcct
     5461 gg
//
LOCUS       DAOQRO010000249         5457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15363_length_5457_cov_6.046279, whole genome shotgun sequence.
ACCESSION   DAOQRO010000249 DAOQRO010000000
VERSION     DAOQRO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15363_length_5457_cov_6.046279"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKK20_09610"
     CDS             <1..416
                     /locus_tag="PKK20_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00183.1"
                     /translation="AWQDEQTPTGTFAWDDQVGEGSARARKVMRGCFLQSIPVAPGQI
                     YVVKAKCRPQGNSLPNLMVRWQTSERQWTHEADDRTFPFQPDQGAWHAGSGTVTVPPD
                     TGRLVVLLNVHGQRTDDDVCWFDELEIYRLPELPF"
     gene            1122..1589
                     /locus_tag="PKK20_09615"
     CDS             1122..1589
                     /locus_tag="PKK20_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00184.1"
                     /translation="MSGASSKSTFYLAEYEFGVDEKRRLQVPAKWRPEDGGEGCEFYL
                     LRWQPTPKKPVCLLVLPPAAFQKLNDKVSALSFSDPNAEALRRSLTRASDVVTLDNAG
                     RMCLPKGLADAAGIRKKAVLVGMLDRFQIWSPEHYEIVRAEDEANTAAALALI"
     gene            2251..3183
                     /gene="rsmH"
                     /locus_tag="PKK20_09620"
     CDS             2251..3183
                     /gene="rsmH"
                     /locus_tag="PKK20_09620"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNV00185.1"
                     /translation="MADDYHQPVLLEEVMAALEPRTGRVYVDGTIGGGGHAEALLERS
                     SPAGRLLGCDLDPTAVSAAAARLGRFAGRFEIRSGNFADLSGWIPAGSSDGVLLDLGV
                     SSHQLDCARRGFSFRVDGPLDMRLDQRRGDTAADLVNNWPVTELARIFRELGEERRAW
                     RVARAVEWERRRTPFRTTRQLADLVERVAPRGGQGIHPATRVFQALRMAVNDELGSLR
                     LGLDAAWAVLKPGGRLAVISFHSGEDRVVKRFGQALAREYEVTGDADIPEWRRPRASL
                     LAWVNRGPVAPSAAEVASNPRARSARLRVMEKLG"
     gene            3190..3558
                     /locus_tag="PKK20_09625"
     CDS             3190..3558
                     /locus_tag="PKK20_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00186.1"
                     /translation="MARKRKTRSAGVRVAPLLLAVGICLFACGSGMGYIWHRNRNAQL
                     GREIQAATAQLEGLRSTNLLLDQQLDGLRSPRALEAAVRNLNLGLVMPQPEQILRIPE
                     GQPGAAVVSSHTLRMAVRRP"
     gene            3574..>5457
                     /locus_tag="PKK20_09630"
     CDS             3574..>5457
                     /locus_tag="PKK20_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV00187.1"
                     /translation="MHLVNTQSIRRIAGLATLLVLGFVGLAGRLAWLQIVEHEELAQI
                     VEKKLQRNEPRPARRGAILDLRGTVLATSVPAKTVCADPSLIYDHHELVAKTLAPLLK
                     VPEPGLLKKLLPQVTMSTNGVLRTNQYVVLKRKVPVDDWERVKRAMVDLDLGLEGKQL
                     STSLKRAVKTLRTRAVFASEDHLRQYPSRSLAAHVIGFTAGGEAETDHGNVFEDHGVT
                     GIELTLDDMLSGVHGWRKRGEEMAPTHGLDVVLTLDANIQNIVEDELARGFERLSPQG
                     MCALVVRPYTGEVLALANLPTFDPNEPGRAANGQRNRATADTYEPGSTFKVVAIVTAL
                     SDGRLTLNETVFCENGRWFYGGQWLRDYRAFGYLSFEEVVSHSSNIGTAKAALRLGEA
                     RLYNTITNFGFGQITGIPLDAQARGRILPPKTWSQISITRIPIGHEISTTPLQMAMAM
                     AAIANGGVLMQPKLVERLQDGAGRIVRQFSPHPLRRVVSESACNEMKRALRSVVSDDG
                     TAMRAQLDYYSVAGKTGTTEKYTHGTYKSGKYYYASFVGFLPVDRPELCILVGVDEPD
                     KSAGQGHTGGVVAAPVFRAIAERTAAYLRIPPDLTPETEGDAKGELSAELAHRRVTSR
                     TAHG"
BASE COUNT          985 a         1512 c         1959 g         1001 t
ORIGIN      
        1 gcgcgtggca ggatgagcag acgccgaccg ggacatttgc gtgggatgac caagtcggcg
       61 aaggctcggc ccgcgcgcgc aaggtcatgc gcggctgttt tctgcagagc atcccggtcg
      121 cgcccggtca gatctatgtg gtcaaggcga aatgccggcc tcaaggcaac tccctcccca
      181 acctgatggt tcgctggcag acgtccgagc gtcaatggac gcacgaggcc gatgaccgca
      241 cattcccttt ccagcctgac caaggcgcat ggcacgctgg ctcggggact gtcaccgttc
      301 cacccgacac gggccgcctg gtcgtcctcc tcaacgttca cggtcagcgg accgatgacg
      361 atgtgtgttg gttcgacgag ttggagattt atcgtttacc ggagctcccg ttctgaccgg
      421 cgcgatctgg tggccctgaa gcctttgatg cgtaacgagg tcggttctta caattgacaa
      481 tcgagcgtgg agcgagcaaa cccggggggc ggtggagaga aggggtgcct atgccgaggc
      541 cgttgcggat tcagtacgcc ggagcgatcg gaaggtcgcc aggcgcgact cgaccgcaac
      601 ggccagcgac cgattggact cttgattgcc gattgtgaga actgacccgt ttccgccctg
      661 aatctgtgag ggatagactc cacaccgatt caggacggaa cacagattga tcccgagact
      721 gaccgaattg gacttccgac aatccctgcc tcgaacgccc ccaaccccag ctcggctacg
      781 ctccagaggt tttcttccgc agaccgctac aaagctaatt cccgcgtgaa gcaaaagccg
      841 aaatgggggt gcgtggcatt tcgcgagagg ccgatgcgtc gagttcaact gccctggtgg
      901 gaggcgggac ttctggagga actcgccaca tgaggaggat ggatggctgc acgccgagga
      961 aggaggccat tgtaagcggt caagaagcaa tgagttccga aagacctgac aagtgttgag
     1021 acaacaattt ggacgcagaa aactattgac taggtggggc aaagtgggta gcagtgttgc
     1081 cttgtggctc ccaagtggtg aacttggagt tctcgttgtc aatgtctggc gcatcgagca
     1141 aatcgacttt ttatctcgcc gaatacgaat tcggtgtgga cgaaaagcgc cgtcttcaag
     1201 tgcccgccaa gtggcggccg gaggatggcg gggaaggctg cgagttctac ttgttgcgct
     1261 ggcagccgac tccgaagaag ccggtttgtc ttttggtatt gccgcccgcg gcgttccaga
     1321 aactgaacga caaagtgagc gcactctcgt tttccgatcc gaatgcagag gcgctgaggc
     1381 gatcgctgac gcgcgcgtcg gatgtggtga cgctcgacaa tgcggggcgg atgtgtctgc
     1441 ccaaggggct cgccgatgca gccgggatac ggaagaaagc cgttctggtt gggatgctgg
     1501 accgatttca gatttggtcg ccggagcatt acgagatcgt ccgggccgag gatgaagcaa
     1561 acacggctgc agcactcgcg ttgatttagc catgagcacg ccctctcaac ttttggctgc
     1621 aggaaggagt ttcatggggg tgcggaaggc gccgcgcccg tttcaggaca gcacgcatgg
     1681 attcctgccg cacttcgagg ccaaaccggt caagcggcgg cctgtcaatc cggagacggc
     1741 ccgactgggg cctttgttct cgcggcttgc agaggatcgc ggtccgaacg cggtggtgga
     1801 tggagggcct gcgcctggcg ggtcggctgc cagggaggag ccgatcgagt ccgggcagcg
     1861 gggggatgcg ggcagggcgg accgggcaaa ggcacctgtg cggcgcgagc ctgccgggct
     1921 gtttcgcggt ctgctgtggt cgggagggtt cgggctgagg tccaaatcgc gcccggcgcg
     1981 catggcggcg cagggggagt tgtcgttggc gacggtgcag gtgaagagga acgacctgca
     2041 cgacgcggat ttggagttgg tttgtgctcc gacttgtgct ggggtgaagt cacagactgc
     2101 gacggcgggg gggccatgcg ggcaaggagc gacagtgagc cgagaggagg ccgatggtgc
     2161 gagctgggct gcgcgggctt ctttctggcg gcggtggttt gagagccgat cggggttgtc
     2221 ccggtaggcc gccggcgcgg cgtatggaac gtggcagacg actatcatca gccggttctg
     2281 cttgaagaag tcatggcggc gttggagccg cgaacaggac gagtgtatgt ggacggcacg
     2341 attggaggcg ggggtcatgc ggaagccctg ctggaacgga gttcacctgc gggaaggctc
     2401 ctgggttgtg acctcgatcc gactgcggtc tcggcggccg cggcccggct tggtcggttc
     2461 gcgggccgat tcgaaatccg atcgggaaac tttgcggacc tgagcgggtg gattcccgcg
     2521 ggatcgagtg acggtgtgct gctggatttg ggtgtcagtt cgcaccaact cgattgcgcc
     2581 cggcgcggat tcagttttcg ggtcgatgga ccgctcgaca tgcggctcga tcagcgtcgg
     2641 ggcgacacgg cggcggatct tgtgaacaac tggccggtca ccgaactggc ccggattttc
     2701 cgcgagttgg gcgaggagag gcgggcgtgg cgggtggcac gggcggtgga atgggagcgt
     2761 cgtcggacac cattccggac gacgcggcag ttggcggatc tggtcgagcg ggtggcgccg
     2821 cgtggggggc agggcatcca ccccgcgacg cgggttttcc aggccttgcg catggcggtg
     2881 aacgacgaac tcgggtcatt gaggctcgga ctggacgctg cgtgggcggt cttgaagcct
     2941 ggcggccggc tggcggtgat cagttttcac tcgggcgagg accgggttgt gaaacgattt
     3001 ggacaagcat tggcgcggga gtacgaagtg acgggtgacg cggacattcc ggagtggcgg
     3061 cggccgcggg cgagcctgct ggcgtgggta aatcgcgggc cggtggcgcc cagtgcggcg
     3121 gaggtcgcat ccaacccgcg cgcccggagc gcccgtttgc gggtcatgga gaaactcggg
     3181 taactcggca tggcaagaaa gcgcaaaaca cgttccgcgg gcgttcgcgt cgcccccctt
     3241 ctgctggcgg tggggatttg tctgttcgcc tgtggatcgg ggatggggta catctggcac
     3301 cgcaatcgca atgcgcagtt gggacgcgag attcaggcgg ccacagcgca attggaaggg
     3361 ttgcgcagca ccaatctgct tctggaccaa caactggacg ggcttcgatc tccgcgcgcg
     3421 ctggaagcgg ctgtgcgcaa tctgaatctg ggactggtca tgccgcagcc ggagcaaatc
     3481 ctgcggattc ccgaagggca acccggcgcg gcggtggttt cctctcacac gctgcgcatg
     3541 gcggtgcggc ggccctgaac aggcatcgac actgtgcacc tggtcaacac ccaatcgata
     3601 cggcggatcg cgggtctcgc gacattgctg gtgcttggct ttgtggggct ggcgggccgg
     3661 ttggcctggc tgcagattgt cgagcacgag gagctggcgc agattgtcga gaagaagctg
     3721 cagcgcaacg agccgcgtcc ggcccggcga ggtgccattt tggacctgcg cggcacggtt
     3781 ctggccacga gcgttccggc caagacggtg tgtgccgatc cgtcgctgat ctatgatcac
     3841 cacgagttgg tggcgaagac cctcgcgcct ttgctcaagg taccggagcc gggcctgttg
     3901 aagaagctcc tcccgcaagt cacgatgtcg accaacgggg tattgcggac caaccagtat
     3961 gtggttctca agcgcaaggt gccggtcgac gattgggagc gggtaaagcg ggcgatggtg
     4021 gatctggacc tgggcctcga aggcaagcag ttgagcacaa gtctcaagcg ggctgtcaag
     4081 accttgcgca cccgggccgt ctttgcctcc gaggaccacc tgcgccagta tccgagccgg
     4141 tcgttggccg cgcatgtcat cggattcacg gccgggggcg aggcggagac cgatcacggg
     4201 aacgtgttcg aggatcatgg cgtgacgggc atcgagttga ctctcgacga catgctcagc
     4261 ggagtgcacg ggtggcgcaa gcggggcgag gagatggcgc cgacacacgg tttggacgtg
     4321 gttttgacgc ttgatgccaa cattcagaac attgtcgagg acgaactggc ccggggtttc
     4381 gagcgtctat cgccgcaggg gatgtgcgcc ttggtggtgc gtccgtacac cggcgaggtg
     4441 ctggcgctgg ccaatctgcc cacattcgat ccgaacgagc ccgggcgcgc ggcgaacggt
     4501 cagcgcaatc gcgcgacggc tgacacctac gagccgggct cgacgttcaa ggtcgtggcg
     4561 atcgtaacag cgttgagcga cgggcggctg acgctgaacg agaccgtgtt ttgcgagaac
     4621 gggcggtggt tttacggtgg ccagtggctg cgcgattatc gcgcgttcgg gtatttgtca
     4681 ttcgaggagg tggtgtccca ctcgtcgaac atcggcacag cgaaggcggc gttgcggcta
     4741 ggcgaggccc gcctgtacaa caccatcacc aatttcggat tcggccagat caccggcatt
     4801 cccctcgatg cccaggcgcg cggacgcatt ctcccgccca agacctggag ccaaatctcg
     4861 atcactcgaa tcccgatcgg acacgagatt tcgacgactc cgttgcagat ggcgatggcg
     4921 atggcggcga tcgccaatgg gggcgtcctg atgcagccga aactggtcga acggctgcag
     4981 gatggggccg gtcgcatcgt gcgtcagttc tcgcctcacc cgctgcggcg cgttgtgagc
     5041 gaatcggcct gcaacgagat gaagcgcgcg ctgcggtcgg tggtgagcga cgacggcacg
     5101 gcgatgcggg cgcagctcga ctactacagt gtggccggca agacaggcac gaccgagaag
     5161 tacacgcacg gcacctacaa gtccggaaag tactactatg cctcctttgt cggctttctg
     5221 cctgtggatc gtccggagtt gtgcatcctg gtcggagtgg acgagccgga caagagcgcc
     5281 ggccaggggc atacgggcgg cgtggttgcg gcgccggtct ttcgggcgat tgccgaacga
     5341 accgccgcgt acctgcggat tccgccggac ctaacgccgg agaccgaagg agacgcgaag
     5401 ggtgagctgt cggcggaact ggcgcaccgg cgcgtcacgt cgcgcacggc ccacggt
//
LOCUS       DAOQRO010000250         5442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15418_length_5442_cov_4.489326, whole genome shotgun sequence.
ACCESSION   DAOQRO010000250 DAOQRO010000000
VERSION     DAOQRO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15418_length_5442_cov_4.489326"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="PKK20_09635"
     CDS             <1..1675
                     /locus_tag="PKK20_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV00188.1"
                     /translation="FRHEQNAGDWANWSRFAKENRYGRHLALGVGAYLNTISNTLVQM
                     TSVRHATASAPPSEGVVCYSYAVPVKDGVPRDEFLAALTGPTSHHPNTPALFPTPVDP
                     PAMPWKSTTGPALLMGYVRDAQTGQPLEGVTIEVCDAATPFLRTDVNGFFGLTRPPNN
                     RLSLVTSAHGYAAQERWIALEPGVTTQVDFHLAPPDPDTAPENIRISTGANSALVSWS
                     TRTPTAGQVLYGLGGPCDSDGATASDQTVATNHTVFLDSLTRRDDNDPRPFRLRVVTW
                     TDRSTNLSNLRLFSPAWPRISDDTEARWTGSWTFVTNSAESLGRAYRRASAVSGTATA
                     ELTWRTRIYTPGLYDLSVLYPAGANGSSGVRYEIESSAGRTAVVVNQAAGGGLYRTVA
                     ANIRFAAGETAIVRLRNNTSSGTGSYVLADAVRWTLRPGQDPPSPGQTPEWWAAHFFI
                     EPSDPALDSDADGYTNYAEFVLGCDPTDPASLFQFRSVSFDDKGWHIRFHPRRQGRAY
                     RLLRAPDIESAVWQPVASASLAAGPDNEGVFTDSAPDPAQTFYRLEVDL"
     gene            1830..3083
                     /locus_tag="PKK20_09640"
     CDS             1830..3083
                     /locus_tag="PKK20_09640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNV00189.1"
                     /translation="MKTGCTSAPPSTSTLSASSIAGVSLVLIALFADGIHAASNADTN
                     RVPGVVIHHSPQSSGIYIGSPSLAVLPNGDYVASHDEFGPKSTEHQRAISHVYRSADR
                     GQSWQRIATINGAFWSTLFTQGDSLYLIGTDKHHGNAVIRRSKDGGRTWTEPRDHRSG
                     LLRDDGQYHCAPVPVIEHRGRLWRGMERRDPPVGWGVNYRAGMLSVAVDADLLDAGQW
                     TFATFLPSNTNWNQGDFGAWLEGNAVVAPDGELVDVLRVQTRSPDERAAIVRVVDEGK
                     TMRFDPDRGFVPFPGGAKKFTIRWDDRASLYWTLATIIPEPFRAPGPGSIRNTLALTA
                     SPDLRTWTVRCILLHHPDTAKHGFQYPDWLFDGEDIIAVVRTAFEDGLGGARNNHDAN
                     FLTFHRITGFRRLTPSDSVPIEMPR"
     gene            complement(3204..4487)
                     /locus_tag="PKK20_09645"
     CDS             complement(3204..4487)
                     /locus_tag="PKK20_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00190.1"
                     /translation="MRFLSDIGPCPPRPAQSKHHLSRRRFLGGALAAGAAASVNAQWA
                     GQTAESPATRSPSAEPGRKIKLGVVGTGGRGCWIAGLFRRHGGYQMWAVADYFQEVAD
                     TCGHGLGVDTSRRFSGLDGYRRLIESGVEAIALETPPYFFPEHARAAVDAGLHVYMAK
                     PVAVDVWGCLEIEAAARKAATLERVFLVDYQIPTDPQNIEVVRRIHAGEIGKVISINS
                     HYFAGLFPDPAKTKNVADRFRGLVWCNDVVLGGGYHVNACIHAVDAALWIAGQRPISA
                     MGCSRVGRGDPHGDSHDVFQLVFEFENGLVLSHRGKHLNNLTDFDVVCQAQGHVGTAQ
                     IAYGGRVASKGPNDGYNGEVQNLYEAGAVRNIAKFYQCVIDRNFENETVRRSVDGALA
                     TILGREAGLRRGKVSMAELLAEKKRLEVDLSGLRV"
     gene            4616..>5442
                     /locus_tag="PKK20_09650"
     CDS             4616..>5442
                     /locus_tag="PKK20_09650"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNV00191.1"
                     /translation="MTFQSTESSATGHWSGRFDDADFQQWATRLRSHLKAPQVSLGLV
                     FMAPWLAPHAAQVLEILRVHAQASLLVGCSGASLIAGGQEIEQHPGLVVSLYHIPGAD
                     VRAVRFTQADVEAEEGPSYWQSQAGLDPENVHGWLVFMDPFTVDGERWLGQWNEAWQG
                     CPTFGGMASGSSNEQGTQVYLDGSVHDDGAIAVAFCGAVSLVGAVSQGCVPIGEPWTV
                     TKAEGNLIHEIGNRPAYEVLARTFNELPRAEQERAQGNLMLGLVTNEYQEEFRRGDFL
                     "
BASE COUNT          965 a         1826 c         1655 g          996 t
ORIGIN      
        1 cttccgccac gagcagaacg cgggcgactg ggccaattgg agcaggttcg ccaaggaaaa
       61 ccgctatggg cggcacttgg ccctgggggt gggcgcctac ctcaacacca tctcgaacac
      121 cctggtgcag atgacctccg tgcgccacgc cacagcaagc gcccccccgt ccgaaggcgt
      181 ggtgtgctac tcgtatgccg tccccgtcaa ggacggtgtg ccgcgcgacg agttcctcgc
      241 ggccctcacc ggccccacaa gccatcaccc caatacgccc gcgctcttcc ccacgcccgt
      301 cgacccgccg gccatgccct ggaagtcaac gactggccca gcgcttctca tgggctacgt
      361 tcgagacgcc caaaccggcc aaccgctcga aggtgtcacg atcgaggtct gcgatgcggc
      421 caccccgttt cttcggactg atgtgaacgg attcttcggc ctgacgcgtc cgcccaacaa
      481 ccgcctgagt ttggtcacat ccgcccacgg ctacgcagcg caagagcgct ggatcgcgct
      541 tgaacccgga gtgaccacac aggtcgactt ccacctcgct ccgcccgatc cggacactgc
      601 gcccgagaac atccgcatct cgacgggcgc aaacagcgcc ctggtctcgt ggtccacacg
      661 cacccccacc gccggccaag ttctctacgg actcggcggt ccctgcgact cggacggagc
      721 aactgcatcc gatcagactg tggccaccaa ccacacggtg ttcctggatt ctctgactcg
      781 ccgcgacgac aacgatcctc ggccattccg tctccgcgtc gtcacatgga ccgaccgctc
      841 gacaaacctc tcgaacctcc gcctcttcag cccggcctgg ccccggattt ccgacgatac
      901 tgaagcccgt tggaccgggt cctggacgtt cgtcaccaac tcggccgaaa gcctcggccg
      961 agcctaccgg cgggcctcag cggtttccgg caccgccacc gctgaactga cgtggcggac
     1021 gcgcatctat acgccgggtc tctacgacct cagcgttctt taccccgccg gcgccaacgg
     1081 atcgtccggc gtgcgctatg aaatcgaatc ctccgccggc cgaaccgccg ttgtcgtgaa
     1141 ccaggcggcg gggggcggac tctatcgcac cgttgccgcc aatatccggt tcgcggcggg
     1201 tgagactgca atcgtgcgtc tgcgcaataa caccagctca gggaccggat cgtatgtcct
     1261 ggccgatgcc gtccgttgga ccttgcggcc cggacaagat ccgccttcgc ccggccagac
     1321 ccccgagtgg tgggccgcgc atttcttcat agagccgtcc gatcccgcgc tcgattccga
     1381 cgccgacggc tacacgaact acgcggaatt cgtgctgggc tgcgatccaa ccgatccggc
     1441 ttctttgttc cagttccgct ccgtgtcgtt cgacgacaaa ggatggcaca tccggttcca
     1501 cccccgccgc caggggcgcg cctatcggtt gctgcgcgcc cccgatatcg aatcggcggt
     1561 gtggcagccg gtcgcttcag ccagtcttgc ggccggccct gacaacgaag gagtgttcac
     1621 ggattcggct cccgacccag cccagacatt ctaccgactc gaagtcgatc tctagcgagc
     1681 gcgcctcaca ccaaatccca gtccttagag ccatgaaaag cccccgtgtt tccccgcccc
     1741 tctctgccgc gcctcgctcc ttggtccgaa tgttgcatga agctccacgg cgcctcttcg
     1801 cattcattcg tcaagtgtcc aggcttcatg tgaaaacagg ctgcacatcg gcgccgccct
     1861 cgacttcgac tctgtcggct tcgtcgatcg cgggtgtgtc tctggtcctg atcgcgcttt
     1921 tcgccgatgg cattcacgca gcttcgaacg ccgacacgaa ccgcgtgcca ggggtggtga
     1981 ttcaccatag cccgcaatcg tcgggcatct acatcgggtc gccaagcctg gccgtgctgc
     2041 ccaacggcga ttacgtggct tcgcacgacg agttcggccc gaagagcacc gaacaccaac
     2101 gcgccatcag ccatgtctat cgctctgccg accgcggcca atcgtggcag cgcatcgcca
     2161 ctatcaacgg cgccttctgg tcaacgctct ttacccaagg cgatagcctc tacctcattg
     2221 gcaccgataa gcaccacgga aacgccgtca tccgccgctc gaaagacggc ggtcggacct
     2281 ggaccgagcc gcgcgaccac cggtccgggc tcctccggga cgacggtcag taccactgcg
     2341 cgccggtgcc cgttatcgag catcggggcc ggctttggcg cggtatggaa cgacgcgatc
     2401 cgccggtggg gtggggtgtc aattaccgcg ccggcatgct ctccgtggcc gtggatgccg
     2461 acttgctcga tgcgggtcag tggacgttcg caacgttcct cccgagcaac acgaactgga
     2521 accagggcga ttttggcgcc tggctcgaag gcaatgctgt cgtcgcgccc gatggcgaac
     2581 tggtcgatgt gctccgcgtc caaacccggt ccccggatga aagagcggcg atcgtgcggg
     2641 tcgttgatga gggaaagacg atgagattcg atcccgatcg cggctttgtc cctttccccg
     2701 gcggcgccaa gaaattcacc attcgttggg atgaccgggc ctcgctgtac tggacgctgg
     2761 ccacgatcat acccgagcca tttcgcgccc ctggtccggg gagcatccgc aacaccctcg
     2821 ccctgaccgc gtcacctgat ctgcgcacgt ggacggtgcg ctgcattctc ctgcaccatc
     2881 ccgacactgc aaagcacggc ttccagtatc ccgattggct ctttgacggc gaggacatca
     2941 ttgccgtcgt gcgcacggcc ttcgaagacg gcctgggcgg cgcgcgcaac aaccacgatg
     3001 ccaacttcct caccttccat cggatcaccg gatttcgccg gctgactccg tccgattcag
     3061 tccccatcga gatgccgcga taacgggtca atcgcgttca aggggtcaag gattctccag
     3121 aacgaggcgc gggagagaag ggcggggaga gcaggggact cctcatgggg aagaggaaga
     3181 ctggatttgc tctgaggcgc tcgctagacc ctcaaaccgc tcaggtcgac ttcaagtcgc
     3241 ttcttctcgg cgagcaactc ggccatgctg accttgcccc ggcgcaggcc ggcttcgcgg
     3301 cccaggatcg tggccaaggc gccgtctaca gagcggcgga cggtctcgtt ctcgaagttg
     3361 cggtcgatca cgcattgata gaacttcgca atgttgcgca ccgcgccggc ttcgtagagg
     3421 ttctgcactt cgccgttgta gccgtcgttg gggcctttgg acgccaccct cccgccgtag
     3481 gcaatctggg cggtgccgac gtggccctgc gcctgacaga caacatcgaa atcggtcagg
     3541 ttgttgagat gtttgccacg atgactcaga acaagcccgt tctcgaactc gaaaacgagc
     3601 tggaacacgt catggctgtc gccgtgcgga tcgccccgac cgacgcgcga acatcccatg
     3661 gcgctgatgg ggcgttgacc ggcgatccag agggctgcat cgacggcatg aatacaggcg
     3721 ttgacgtggt agccgccgcc cagcaccaca tcgttgcacc agaccaggcc gcggaagcgg
     3781 tcggcgacgt tcttcgtctt ggccggatcg ggaaacaagc cggcgaagta atggctgttg
     3841 atcgaaatca ctttgccgat ttcacccgcg tggatgcgcc tgacgacttc gatgttctgg
     3901 gggtcggtgg ggatctggta atcgaccagg aacacccgtt cgagagtggc cgccttgcga
     3961 gccgcggcct cgatctcgag acagccccac acatcgaccg ccacgggctt cgccatatat
     4021 acgtgcaaac cggcgtcgac cgccgcccgg gcatgttcag ggaagaaata cggcggcgtc
     4081 tcgagagcga tggcctcgac gcccgattcg atcagtcgcc ggtaaccatc aaggcccgag
     4141 aagcgacgcg aggtgtccac tcccaagccg tggccgcagg tgtcagccac ttcctggaag
     4201 tagtccgcca ctgcccacat ctggtagccg ccgtgccgac gaaacagccc agcgatccag
     4261 caccctcggc cccctgtgcc cacgactccc agcttgatct tgcgaccggg ctcagcggac
     4321 ggacttcgag tcgcagggct ttccgctgtt tgcccggccc actgggcgtt gaccgatgcc
     4381 gcggcgcccg cggccagggc gccccccaga aaacgccgcc ggctcagatg atgcttggat
     4441 tgagccggtc tgggtgggca gggaccgatg tcagataaaa agcgcataag aattgtatgt
     4501 tggtcgccgt ggttttctgc gggcaacttc agaccaagcg cccgcggttg gcaagctcgg
     4561 acgacggggt gctaaggttc tggatttccg atccgagcgg ggttactgtc cggcgatgac
     4621 ttttcagtca accgaaagtt ctgcaacggg gcactggtcc gggcgcttcg acgatgccga
     4681 tttccagcag tgggcgaccc ggttgcggtc gcacctgaag gccccgcagg tttcgctggg
     4741 cctggtgttc atggcgccct ggctcgcgcc gcacgcggcg caagtgctcg aaatcctccg
     4801 tgtccatgcg caagccagcc tgttggtggg atgttcgggc gcaagcctca tcgccggcgg
     4861 tcaggagatc gagcagcacc ccggattggt cgtcagtctg taccacattc ccggggcgga
     4921 tgtgcgggcg gttcggttca ctcaggcgga cgtcgaagcc gaggaggggc cctcctattg
     4981 gcagagtcag gccgggcttg accctgagaa cgtccatggc tggctggtgt tcatggatcc
     5041 gtttacggtg gatggcgagc ggtggctagg ccagtggaac gaggcgtggc agggatgccc
     5101 caccttcggt gggatggcca gcgggtcttc gaacgagcag gggacccagg tatacctcga
     5161 tggcagcgtc catgacgacg gcgcaatcgc cgttgccttc tgcggcgcgg tgagccttgt
     5221 cggcgcggtc tcccaggggt gcgtccccat cggcgagccg tggaccgtga ccaaggccga
     5281 agggaatctg attcacgaga tcggtaatcg tccggcctac gaggtgctgg ccaggacatt
     5341 caacgaattg ccccgcgccg agcaagagcg cgcccagggc aacctgatgc tcggtttggt
     5401 gaccaatgag tatcaggagg aatttcggcg cggcgatttc ct
//
LOCUS       DAOQRO010000251         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15544_length_5417_cov_4.578888, whole genome shotgun sequence.
ACCESSION   DAOQRO010000251 DAOQRO010000000
VERSION     DAOQRO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15544_length_5417_cov_4.578888"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..694
                     /locus_tag="PKK20_09655"
     CDS             116..694
                     /locus_tag="PKK20_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV00192.1"
                     /translation="MNIDSELQKHYAQLLGVGSPWEVKNVELKPAEKRVEIELGWQWG
                     ADAQCPECGCRCSIHDCAPERTWRHLDTMQFRTEIRARVPRADCPEHGVRTMQVPWAA
                     PHRRLTLLFERFAVDVLLASASVNQGCKLLGIGWESAREILRRAVERGLERRQLEGLK
                     RLAMDEKSFGRGQSYIRLLSDLELSRVLDVVE"
     gene            1204..4107
                     /gene="gcvP"
                     /locus_tag="PKK20_09660"
     CDS             1204..4107
                     /gene="gcvP"
                     /locus_tag="PKK20_09660"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015202967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNV00193.1"
                     /translation="MINGTDSTDLPLHPLPAEAFAHPDRFVRRHIGPRPDDIRAMLGT
                     LGFASLDAMTQRAVPGAIRRRTALRLPEPLSEFEALVRLRAIAGQNRLFRSFIGQGYY
                     DCITPPVIQRNVLENPGWYTPYTPYQSEIAQGRLEGLLNFQTLVCSLTGMEIANASLL
                     DEATAAAEAMMMCHRLKAAEGRNVFLVAAGVFAQTLAVVRTRAGALGVKVRSVEPSRM
                     TFSPEVLGVLLQSPSAGGAIEDDEELIRRAHAAGALAVVATDLLALALLRPPGESGAD
                     IVVGSAQRLGIPLGFGGPHAGFFATRDAFKRQMPGRLVGISKDSRGRPAYRLALGTRE
                     QHIRRDRATSNICTAQALPANIAAAYAVYHGPDGLRSIARRVHRLTAALAAGIHKLGR
                     RVVNPTFFDTLTIEVGAGGSAAVHRAAADRSVNLRAVDADHVGISLDEATSIDDIRLL
                     LEILNGGQPTPFDVATLSIAEPALAPGRQTPFLEHPVFHRYHSETDMLRYLRRLESRD
                     LSLCQAMIPLGSCTMKLNATSEMLPITWPEFSRIHPFAPVDHTQGYQRLFRDLETWLA
                     EITGLDAVSLQPNAGAQGEYTGLLIIRRYHEQRGERHRNVCLIPVSAHGTNPASAVMA
                     GFKVVAVSCDDEGNVDLADLGAKAQSHANALAALMVTYPSTHGVFEPGIHEVCRIIHE
                     HGGQVYMDGANMNALVGLTSPGTMGADVCHLNLHKTFCIPHGGGGPGVGPICVARHLV
                     DYLPDHPLAGPSRNQSIGAVSAAPWGSASILPISWLYIACMGATGLTEATQVAILNAN
                     YLAHRLRDFYPVVYKGPGEMVAHECILDFRSFGKVTAEDVAKRLMDYGFHAPTLSWPV
                     AGTLMVEPTESESKEELDRFCEAMIAIHAEIAEIESGRVDTQNNLLKNAPHTADMLAA
                     DSWTRPYTRAQAAYPTRHQLEFKFWPAVGRVDNVYGDRNPVCTCVGMDAYTGA"
     gene            4239..4829
                     /locus_tag="PKK20_09665"
     CDS             4239..4829
                     /locus_tag="PKK20_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNV00194.1"
                     /translation="MNDWITGYVAAQKAALDSIPTDAVARLIRTFQKAWREDRQMFVF
                     GNGGSAATASHFATDLGKGASDKLPRRFRVLSLNDNVPWMTALGNDYSYEDVYLRQLQ
                     NYARPGDLALTMSVSGNSPNLVKAIHWAAANGLHTIALVGSKRGRLADLAHDVIVIDD
                     THYGRVEDCHMGICHMLCYAFMERPEMAASNGAAGS"
     gene            complement(5077..>5417)
                     /locus_tag="PKK20_09670"
     CDS             complement(5077..>5417)
                     /locus_tag="PKK20_09670"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNV00195.1"
                     /translation="QEMLSPTSYIVGMGLGDKVALITDGRFSGGTAGACIGHVSPEAA
                     EGGPIGLLKPGDRIRIDFPNRRIDSLLSDVELAERKRVWQPVRRDLSGWLARYRKLVT
                     NASHGGILNA"
BASE COUNT          957 a         1690 c         1769 g         1001 t
ORIGIN      
        1 gttgggatca accgcttcgc ggcggacttc gctccgctcc gctgcgctcc gccggtgtga
       61 gcctttgggc ggaagaggct ggtgaaggag ctggagtttt gggaagctgg cgctcatgaa
      121 catcgatagc gaactacaaa agcattacgc gcaactgttg ggcgtgggca gcccgtggga
      181 agtcaagaac gtggaactga agccggccga gaagagggtg gagatcgaac tgggctggca
      241 gtggggtgct gacgcccaat gcccggagtg cgggtgtcgg tgttcgattc acgattgcgc
      301 gccggagcgg acctggcggc atttggatac gatgcagttt aggaccgaga ttcgggcgcg
      361 tgtgccgcgg gcggactgcc cggaacacgg ggtcaggaca atgcaggtgc cctgggcggc
      421 gccgcatagg cgcttgacct tgctctttga gcgttttgcc gtcgatgtgt tgctggccag
      481 cgccagcgtc aaccaaggct gcaagctttt gggcattggg tgggagagcg cgcgcgagat
      541 tctgcgccgg gccgtcgagc ggggcctgga gcggcggcag ttggagggtc tcaagcgcct
      601 tgcgatggat gagaagagct ttgggcgcgg tcaatcctac atcaggttgc tgagcgacct
      661 ggagctgtcg cgggtgttgg acgtggtgga gtaaagaggt cgtggcgatt gccaacgcat
      721 ttactgccac gaatatgctt gcaagaggcg atccaggtgg cgcaggattc ggggcgcgtc
      781 gctttacttg ccgttatttc ttgggagaag cagactcaga ctctgcctga aataaccgcc
      841 cttgaggaag aggggaagtc ggctgcagtg gtctttaccg tactatttac tccggctaat
      901 gaaggccggt gaatgtggtg ggctgggcgg tgtttccgag gctgatgtgg gcggcggggc
      961 gtggcgggtg gcgtggggcg cgtggtggag cggcggtgta ccacggcggc tcgcatcgga
     1021 gtgcgtgtat tgcccgaacg cgtggtggaa ttgtttccgg agggcacgcc tcggggcgtg
     1081 ttcgatgccg ttagagcgct taaacacgcc agtccgaagg atcgccccga ctcgctcttc
     1141 gccacagtga gggtgacgcc gtcaacggaa ccgacgacga tttcacagac tgacgatact
     1201 accatgatca acggcaccga ttctaccgat ttgcccctcc accccctgcc cgcggaggcc
     1261 tttgcccatc cggatcgttt tgtgcgacgc cacatcggtc cgcgccccga cgatattcga
     1321 gccatgttgg gaaccctcgg gtttgcgagt ctcgacgcga tgacccaacg agctgttccg
     1381 ggcgcgattc gccgtcggac tgcgctgcgt ctgcccgagc cgctctccga atttgaggcc
     1441 cttgtccgac tgcgggccat tgcggggcaa aaccggctct tccgctcctt cattgggcag
     1501 ggctactacg actgcatcac gccgccggtg attcagcgta acgtcctcga gaatccgggc
     1561 tggtatacgc cgtacacacc ctaccaatcg gaaatcgccc agggccggct cgagggattg
     1621 ctcaacttcc agacgctggt ctgcagcctg accggcatgg agatcgccaa cgcctcgctc
     1681 ctggacgaag ccacggccgc ggccgaggcg atgatgatgt gccatcggct caaagcggct
     1741 gaggggcgca acgtcttcct cgtagccgca ggggtctttg cgcaaaccct cgccgtggtc
     1801 cgaacccgcg ctggcgccct gggcgtaaag gtgcgctccg tcgagccttc gcggatgacc
     1861 ttctcgccgg aggtactggg tgtcctgctg caatcccctt ccgccggcgg ggcgatcgag
     1921 gatgacgagg agttgattcg tcgggcgcac gccgccgggg cgctggcggt cgttgcgacc
     1981 gaccttctgg ctctcgccct gcttcgtcct cccggcgagt cgggagcgga catcgtggtc
     2041 ggcagcgccc agcgactggg gattccgctc ggctttgggg gcccgcacgc ggggttcttc
     2101 gcgacgcggg atgcgttcaa gcgacagatg cccgggcgcc ttgttggcat ctcgaaggac
     2161 tcgcgcggtc ggccggcgta ccggcttgcg ttgggcacac gcgagcaaca tatccgccgc
     2221 gatcgagcca ccagcaacat ctgcaccgcg caagccttgc cggccaacat cgccgccgcc
     2281 tatgcggtct atcacggccc ggatggcctc aggtccatcg cgcggcgtgt ccaccgcctt
     2341 accgccgcgc tggcagcagg aatccacaaa ctcggacgcc gtgtcgtgaa tccgacgttc
     2401 ttcgacacgc tcaccatcga ggttggcgcg ggcggctccg ctgcggtgca tcgggcggcc
     2461 gccgatcgga gcgtcaacct gcgcgctgtg gatgccgacc acgtcggcat ctcgctcgat
     2521 gaagccacat cgatcgacga catccgactg ctgctcgaga ttctgaatgg cggccaaccg
     2581 acgcctttcg atgtcgccac cctgtcaatc gcggagccgg cactcgctcc tgggcgccag
     2641 actccgtttc tcgaacatcc tgtcttccat cgctatcatt ccgagaccga catgctgcgg
     2701 tacctgcggc gcctcgagtc gcgggacctt tcgctctgcc aggccatgat cccgctggga
     2761 tcgtgcacga tgaagctgaa cgcgacttcc gagatgctcc ccatcacctg gcccgagttc
     2821 agccgcatcc atccgttcgc gcctgtcgac cacacgcaag gctatcagcg cctgttccgc
     2881 gacctcgaga cctggctcgc cgagatcacc ggcctggacg ccgtgtcgct ccagcccaac
     2941 gcaggggccc aaggcgagta cacgggtctg ctgatcatcc gccgctacca tgaacagcgg
     3001 ggggagaggc atcggaatgt ctgtctgatc cccgtctcgg cgcacggcac gaatcccgcc
     3061 agtgcggtca tggccgggtt caaggttgtg gcggtgtcgt gcgacgacga gggcaacgtg
     3121 gatttggccg acctgggggc gaaagcccaa tcccatgcga atgccctggc cgcgttgatg
     3181 gtcacctacc cttcgacaca cggggtgttc gaacccggga tacatgaggt ctgtcggatc
     3241 atccacgagc atggagggca ggtgtacatg gacggagcaa acatgaacgc cctggttgga
     3301 ctgacaagcc ccgggacaat gggggccgac gtctgccact tgaatctcca caaaacgttc
     3361 tgcattccgc atggcggggg cgggcccggg gtcggcccca tttgcgtggc caggcacctc
     3421 gtggattatc tccccgacca tccgttggcc gggccgagcc gcaaccagtc gatcggagcg
     3481 gtatcggccg cgccgtgggg cagcgcgagc atcctgccga tttcgtggct ctacattgcg
     3541 tgcatgggcg cgacgggcct gaccgaggcc acgcaggtcg cgatcctcaa tgcgaactac
     3601 ctggcccatc gcctgcgcga cttctatccg gtggtgtaca aggggccggg cgagatggtc
     3661 gcccacgagt gcattctgga tttccgttct tttggaaagg tcacggccga ggacgtcgcc
     3721 aaacggctca tggactacgg attccacgcg ccgactctga gttggcccgt ggcgggcacg
     3781 ctgatggtcg aaccgaccga gagcgagtcg aaggaggaac tggaccggtt ctgcgaggcc
     3841 atgatcgcga ttcacgccga aatcgcggag atcgaatcgg gccgggtgga cacgcagaac
     3901 aacctgctca agaacgcgcc gcacacggcc gatatgctcg ccgcggactc gtggactcgg
     3961 ccctacacac gcgcccaagc cgcctatccg acgcggcatc aactcgaatt caagttctgg
     4021 cccgcggtcg ggcgcgtgga caacgtgtac ggcgatcgca acccggtctg cacctgcgtc
     4081 ggaatggacg cctacacagg cgcttgaatt cccatgtcaa cagccaatgc gcatgcatct
     4141 caaagcccgc cctccggcag gcctccgcca gcgtcttgat cccgccgagc cgcagccgag
     4201 actggtcaaa cgcgcgggtt ctgccattct ccaggcgcat gaacgactgg attaccggtt
     4261 acgtggccgc gcagaaagcg gccctcgatt cgatccccac cgatgccgtg gcgcgtttga
     4321 tacgcacctt tcaaaaggca tggcgtgagg atcgccagat gttcgtcttt gggaatggag
     4381 gcagcgccgc caccgcgtcc catttcgcca ccgatctggg caagggcgct tcggacaaac
     4441 tgccgcggcg ttttcgggtc ttgtcgctca atgacaatgt gccctggatg acagcgctcg
     4501 gtaatgacta ttcgtacgaa gacgtgtatc tgcggcaact ccagaactac gcccggcccg
     4561 gcgatctggc gctgaccatg agtgtgagcg ggaattcacc aaatctcgtc aaggccatcc
     4621 actgggcggc tgccaacggg ctgcacacga ttgcgctggt cgggagcaaa cgcgggcgcc
     4681 tcgccgatct cgcccacgat gtgattgtga tcgacgacac gcattacgga cgggtcgagg
     4741 actgtcacat gggcatctgc cacatgctct gctacgcctt catggaacgg cccgagatgg
     4801 cggcatcgaa cggcgccgcc ggctcatgaa cgttggatcg ttggcacgga acccggttca
     4861 gacccgccgc aagcatcgag gaagggcaag ccaatcagcc ccaagacggc caatggcaac
     4921 ccgcccagcc gaaaaaactg gcgctcgaat tccgactctc aacgttcgac aacgcgcttt
     4981 gtcgagagcc ggacgccgag catcgagcgt cgggcgtttg attcatttca gcccggatga
     5041 ttccaaagct ccgcggagtc tcacatccgg gcgctctcaa gcgttgagga tgccgccatg
     5101 gctggcgttg gtgaccagtt tgcgatagcg cgccagccat ccggacaagt cgcgccgaac
     5161 aggctgccag acacgtttgc gctcggccag ttcgacgtcg gacagcaggc tgtcgatgcg
     5221 ccggtttggg aagtcgatgc gaatccggtc gcccggtttg aggagtccga tggggcctcc
     5281 ctcggcggct tcgggcgaga cgtggccgat gcaggcgccc gcggtgccgc cgctgaaccg
     5341 tccatcggtg atgagcgcga ccttgtcgcc cagccccatc ccgacgatgt agctggtggg
     5401 agagagcatt tcctgca
//
LOCUS       DAOQRO010000252         5411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15575_length_5411_cov_6.038835, whole genome shotgun sequence.
ACCESSION   DAOQRO010000252 DAOQRO010000000
VERSION     DAOQRO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15575_length_5411_cov_6.038835"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(967..1683)
                     /locus_tag="PKK20_09675"
     CDS             complement(967..1683)
                     /locus_tag="PKK20_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV00196.1"
                     /translation="MCSPRDKGQAPRNLPREAPSALEGSLFHDSLNTTGGLGGLLEAL
                     ASQALEDLRPDLEIAQTGFRDMVDQIHAQRDLSRAETNELIAVPTRLSSLQVLRRIVE
                     QYRKHPLAAEREIVLDPLAVDHEFTSDRTLLGRALNNLLKNALEACPAGGAVRLGCEK
                     TGETLCFWVRNPGVMPRTVQLQVFNRSFSTKGEGRGLSTYAVKLLTERYLAGSVSFTS
                     RPEDETIFRVTLPITLASVG"
     gene            1715..3550
                     /gene="typA"
                     /locus_tag="PKK20_09680"
     CDS             1715..3550
                     /gene="typA"
                     /locus_tag="PKK20_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNV00197.1"
                     /translation="MQHIRNIAIIAHVDHGKTTLVDCLLKQSGTFRANEAIAREERIM
                     DSMDLEREKGITIRAKNAAFKYRNYHINIVDTPGHADFGGEVERAMSMIDGVLLVVDA
                     ADGPQAQTRFVLRKALEAGAKPIVVINKIDREHANPKKVLDQVFDLFVQLGATDEQLD
                     FPFVYASAKDGYAQLELDHVRESMEPLFETIVKRVPPPRAFASQGFQMLVANLDYSDY
                     LGRIAFGKIHSGKVVQGQPAVCIHANGSKTEGKITMIYHFEGLKRIEVSEAHAGDIAG
                     LTGFEDVFIGETLADSPDRPALPSKPIDPPTIQMEFAVNDGPLGGKDGKLVTARHIGE
                     RLVKETRTNVALRVEPTSDPKVFNVSGRGEMQIAILVEQMRREGYEVLVSRPEVLWRE
                     GADGILLEPIERLFIEMPDDYMGAVMENLSWRKAEIINVTHHTHHISIEALIPMRGLI
                     GFESDLINLTRGLGAMSHLFEQYGPDRGEIAARKNGSLVSIEDGIATAYALNMNQERG
                     RLMIEPGDRVYKGMIVGENARENDIPVNPCKTKHLTNMRSQGEGKGIQLEPPLKMSLE
                     RALEYIGSDEYVEATPRHLRLRKKILDEVQRKRAAQNRAQRVVLE"
     gene            complement(3959..4807)
                     /gene="tsf"
                     /locus_tag="PKK20_09685"
     CDS             complement(3959..4807)
                     /gene="tsf"
                     /locus_tag="PKK20_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNV00198.1"
                     /translation="MAEISATLVVKLREMTSAGMMDCKRALVETNGDLAAAVDLLRKR
                     GVATAAKKAQREAREGAIAQCILPGARVGVLLEVNCESDFVAKNEMFRAFCDQAVRAL
                     AENPAASLETLRTDAIARIRENIKIARHQRFEVSGNGLVAAYIHTGAKVGVLVEVGAG
                     NEATTTRDDFRQLVKDITLQIAAASPVAVSRDQMDPAIVAKEKEIVAASDLLKNKPPQ
                     ALAKIIEGKLEKFFQTACLLDQGFVKRNSEITVGEHLATVAKQLDDQITIRRFVRFQV
                     GEAFAS"
     gene            complement(4898..>5411)
                     /gene="rpsB"
                     /locus_tag="PKK20_09690"
     CDS             complement(4898..>5411)
                     /gene="rpsB"
                     /locus_tag="PKK20_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNV00199.1"
                     /translation="GSIGDYGKQEQSMFRRELARLLKNLDGIRDMEALPSALFVVDIK
                     REHNAVAEARRLRIPVVAIVDTNCDPTEADYPIPGNDDAIRSVRIILALITQTIGQAR
                     AEYEAKHARRVSEAERAAAETPAQPAASDGAAALAAEPTPPVEAVPAAPLPAPVLAPE
                     VQPVPTAPAN"
BASE COUNT         1019 a         1656 c         1753 g          983 t
ORIGIN      
        1 cgtccgcggc tacgcgacgg ccgcgggacg gctgcgcctc cgtagccgcc gtcgtgagac
       61 ggcgcgatgt ctgcttttga ccggcagcag ccacgcctgg tcctgcggcg ctcgactgga
      121 accgagacac tgttccgacg tgggccggcg cgccgggtta gccagccttc ttgtcatcat
      181 gccctttctg gataagcatc tcaattcgtc gcaaacgctg tgcggactgt gaggtactct
      241 caatcacccg tgcgaatccg agcaaactca atccgctgac aactccgccg agaaaaacaa
      301 cccaacctgc gactgtgttc accgagccaa ctccgagtcc tgcaagtggt gcggcgacga
      361 gctcgaaaac agccatgcat gtcagaaaag cggcaactcc cgaaccctcc ataggcggca
      421 gttcgagtcc cggaggttgc ggcccgggca ctggtgcggg aatagtccct gcattcggtg
      481 gccgctctgg cggcttcggt cgctcgatag gacggccacg cagtggaact aagatggtct
      541 ctttgcatgt cgggcatgca atcgactgcg tggccaactc ttctggtgcg tcaagtgtct
      601 gaccgcagtt ggaacaatgg aaacgtatgt ctggcatagt gcgtgcggta gccggctaac
      661 agttagcaat gtatccgtcc cggcggggcc acgggcacac gattccgtcc cggtcgttct
      721 tcccaccggg acgaatacat tgatgttgga cgaagccgca gccctcgcgc gggaattcac
      781 aaggaaaagt aagcccaaga ccaggccgcg aaaagcaaga aacctcgcag ccgcacgtcg
      841 gcgcggccac cttcggcatt cggatcctcg gcgtcagcgc cccaccacag ccgccttccc
      901 cgcagagtgc gccccaagag cggaccagcg ccctgcgggc gcgaaaccct gcaaccgtca
      961 tcggattcaa cccaccgaag cgagcgtgat cggcaaggtc acgcggaaga ttgtttcgtc
     1021 ttctggccgg ctggtgaaac tgacgctgcc cgcgaggtat ctttcagtga gcaatttgac
     1081 cgcataggtg ctcaaccccc ggccttctcc cttggtcgag aacgagcggt tgaagacctg
     1141 cagttggacc gtgcgcggca tgacgccggg gttgcgcacc cagaagcata gagtctcgcc
     1201 cgtcttttcg cagcccagcc gcaccgcgcc gccggcgggg caggcctcga gggcgttctt
     1261 gaggaggttg ttcagcgccc gtccgagcaa cgtccgatcg ctggtgaatt catgatccac
     1321 agccaaaggg tccagaacga tctcacgctc ggcagccaac gggtgcttcc gatactgttc
     1381 gacgatcctc cggaggacct gcaacgatga aaggcgcgtg gggacagcga tcaactcgtt
     1441 ggtctcggct ctcgacaggt cacgttgcgc gtgtatctgg tccaccatat ctcgaaagcc
     1501 ggtctgggca atttcaagat cgggccgcaa gtcctcgagc gcctggctgg cgagggcttc
     1561 gagcagtccg cccaggccgc cggtggtgtt cagcgaatcg tgaaagagac tcccttccag
     1621 cgctgagggc gcttctcgtg gcagatttcg cggcgcttga cctttgtcgc ggggggagca
     1681 cactgccggg ctcgattgac gcgattcgcc ctatatgcag cacattcgga acatcgccat
     1741 tatcgcccat gtcgaccacg gcaagacgac actggtcgat tgccttttga aacagtccgg
     1801 gacattccgg gccaacgagg cgatcgcacg cgaggaacgg atcatggact cgatggatct
     1861 cgagcgcgag aaggggatca caatccgggc gaagaacgcc gcgttcaagt acaggaatta
     1921 ccacatcaac atcgtcgata cgcctgggca tgcggatttc gggggcgagg tcgagcgggc
     1981 gatgagcatg atcgacggcg tgctgctggt tgtggacgcg gcggatggtc cgcaggctca
     2041 gacgcgcttt gtgttgcgga aggcgctcga agcgggcgcc aagccgattg ttgtgatcaa
     2101 caagatcgac cgggagcacg caaatcccaa gaaggtgctc gaccaggtat tcgatctgtt
     2161 cgtgcaactg ggggcgacgg acgaacagct cgatttcccc tttgtgtacg ccagcgcgaa
     2221 ggacggctac gcccagctcg aactcgatca tgtgcgcgag tcgatggaac cgctcttcga
     2281 gaccattgtg aagcgcgtcc cgccgccgcg ggcctttgcg agccagggtt ttcagatgct
     2341 tgtggccaac ctggattatt ccgattatct cgggcgcatc gcgttcggga aaatccacag
     2401 cggcaaggtg gtgcaggggc agcctgcggt ctgtatccac gcgaacggtt cgaagaccga
     2461 gggcaagatc acgatgatct accacttcga gggactcaaa cgcatcgagg tgtccgaggc
     2521 ccacgccggt gatatcgccg ggctgaccgg gttcgaggat gttttcattg gcgagacttt
     2581 ggcggacagc ccggatcggc cggccttgcc gtccaagccg atcgatcccc ccaccatcca
     2641 gatggaattc gcggtcaatg acggtccgct ggggggcaag gacggcaaac tcgtcacggc
     2701 gcgccatatc ggagagcggc tcgtgaagga gacacgcacc aacgtggctc tgcgggtcga
     2761 gcccacgtcc gacccgaagg tgttcaacgt gagcggacgc ggcgagatgc agattgcgat
     2821 cctcgtcgag cagatgcgcc gggaagggta cgaggtgctc gtctcgaggc ccgaggtgct
     2881 ctggcgcgag ggcgcggacg ggattctgct cgagccgatc gagcggctgt tcatcgagat
     2941 gccggacgac tacatggggg ctgtgatgga gaacctttcg tggcgcaagg cggagattat
     3001 caacgtgacc catcacaccc atcacatcag tatcgaggcc ctgatcccga tgcgcggcct
     3061 gatcggtttc gagagcgacc tcatcaatct cacccgcggt ctgggcgcga tgagccacct
     3121 cttcgagcaa tacgggcccg accggggtga gatcgccgcc cgcaagaacg gctcgctggt
     3181 cagcatcgag gacggcatcg ccacggccta cgcgctcaac atgaaccagg aacgcgggcg
     3241 cctgatgatc gagccgggcg atcgggtcta caagggcatg atcgtcggcg agaacgcccg
     3301 cgaaaacgac atcccggtca atccctgcaa gaccaaacac ctgaccaaca tgcgctctca
     3361 gggcgagggc aagggcatcc agctcgagcc cccgcttaag atgtcgctcg aacgagccct
     3421 cgaatacatc gggtccgacg agtacgtcga agccacaccg cgtcatctgc gcctgcgcaa
     3481 gaagattctc gatgaggtcc aacgcaagcg cgccgcccag aaccgcgccc agcgggtcgt
     3541 gctggaatag cgcagggggg gcgagaaaag tctcggcgaa gggtggatgg gcatggcgtt
     3601 gttggcatcg ggttcctgat ggaaaaagcg tgttcgatcg ggtagtgtcg ccgtcagaat
     3661 aaagacggtc gcgctcctgg ccgcggcggg cgtcagtgca gcgctggccc agccattcgc
     3721 actcgatccc gatttcccca acagcagagg cgccgatgac ttcgtccgat cggttgcggt
     3781 ccagcccgac ggacgcattg ttgtggccgg ggatttcacc aatgtgcacg gcgcatttcg
     3841 ccccggcatg gcgcgtctcc tggccgacgg ccgactggac gattccttca agcccgcggg
     3901 atgggcatca gaagaggatg cgccggcgca tccttcgaat cgggtccggc tcgacgggtt
     3961 aactggcgaa ggcctcgccg acctggaatc ggacgaaccg gcggatcgtg atttgatcgt
     4021 cgagttgctt ggccacggtg gccaggtgct cgcccaccgt gatctcggaa ttgcgtttga
     4081 cgaagccctg atcgagcagg caggcggtct ggaagaactt ctcgagcttg ccctcgatga
     4141 tcttggcaag ggcctgaggc ggcttgttct tgagcaaatc cgaggcggcg acgatctcct
     4201 tctccttggc cacgatggcg ggatccatct ggtcgcgcga gacggcgacc gggctggcgg
     4261 cagcgatctg caaggtgata tccttgacca attgccggaa gtcgtcgcgg gtcgtggtgg
     4321 cctcgttgcc agccccgacc tcgaccagca cgccgacctt ggcgccggtg tggatatagg
     4381 cggccaccag gccgttgccc gagacctcga accgctgatg ccgcgcgatc ttgatgttct
     4441 cgcgaatgcg ggcgatggca tcagtgcgca gcgtctcgag actcgcagcc ggattctccg
     4501 ccagcgcgcg caccgcctga tcgcagaagg cgcggaacat ttcgttctta gcgacgaaat
     4561 cgctctcgca attcacctcg agcagcacgc ccacgcgcgc gccgggaagg atgcactggg
     4621 cgatggcacc ctcccgggcc tcgcgctgcg ccttcttcgc cgcggtggcg acaccgcgtt
     4681 tgcggagcaa atcgactgcg gcggcgaggt cgccgtttgt ttccaccagc gctctcttgc
     4741 agtccatcat gccggcgctg gtcatttcgc gcagcttaac aaccagggtt gcactaattt
     4801 cagccataca aaaacagtct gttcaggtgt gttcggacac gggatggcga cgcgtggttt
     4861 gtcgccaggc cgcccgtgtg tcctcgagtt aacttcttca gttcgccggg gcggtcggca
     4921 caggttgaac ctcgggcgcc aacaccggcg cgggaagcgg cgcggcggga accgcctcga
     4981 ccggcggggt cggttcggcg gcgagggcgg ctgcgccatc ggacgccgcg ggctgggccg
     5041 gggtctcggc ggcggctctt tccgcctcgg agacacgccg ggcgtgcttg gcctcgtact
     5101 cggcgcgtgc ctggccgatc gtctgggtga tcaaggcgag aatgatgcgt acggaccgga
     5161 tggcatcatc attgccggga atcggatagt ccgcttcggt ggggtcgcag ttggtgtcga
     5221 cgatggcgac gaccgggatg cggaggcggc gagcctcggc cacggcgttg tgttcccgct
     5281 tgatatcgac tacgaacagg gcgctcggca gcgcttccat gtcgcggatg ccgtccagat
     5341 tcttgagcag acgggccagt tcgcgtcgga acatcgattg ctcctgcttg ccataatcgc
     5401 cgatcgagcc g
//
LOCUS       DAOQRO010000253         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15687_length_5383_cov_4.406532, whole genome shotgun sequence.
ACCESSION   DAOQRO010000253 DAOQRO010000000
VERSION     DAOQRO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15687_length_5383_cov_4.406532"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKK20_09695"
     CDS             complement(<1..556)
                     /locus_tag="PKK20_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNV00200.1"
                     /translation="MRIRRANHLGMCFGVRNAIRLALDRARSQPLTVLGDLVHNETVL
                     GLLRRHGIRTVRLGESIATSAVMVTAHGASNRTKAALRARGLDVIEATCPLVRHAHLA
                     ILALARAGYHPVVVGQRDHVEVRGLTGDLDAFDVILTDQDVRQLRPHPRFGIAAQTTQ
                     PSDHVRHLAGLLRERFPEAEVKYVD"
     gene            complement(612..920)
                     /locus_tag="PKK20_09700"
     CDS             complement(612..920)
                     /locus_tag="PKK20_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNV00201.1"
                     /translation="MNPEPFLQLDRVVHEKGRLPIVTLLAANAELSFTEIRDTLQMTD
                     GNLSVHLRTLQEAGYVAITKSFYARRRLTTCSLTPAGRAAFRQYINLLEQIVREAKRV
                     "
     gene            complement(917..1600)
                     /locus_tag="PKK20_09705"
     CDS             complement(917..1600)
                     /locus_tag="PKK20_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00202.1"
                     /translation="MQSDLATEHLQVIRILMERSILYRRALAPMMLLAGALGTAAAVV
                     GGWAQIGAEEAAAHRFVVYWLAVALATMSAALVLVRRQALRASEPFWSLPTQRVVRAG
                     LPAAGSGLVLGLMVYGVLVLEPQEAGPWRAAAMRELPMAWAVLYGCALHAAGFFMPRG
                     IKWFGWVLILGGWALFAGVHLPVVEWVGLREVGRCLWAHGVMGLFFGVLHLIYGVYLT
                     CTEPKALSA"
     gene            1888..2916
                     /locus_tag="PKK20_09710"
     CDS             1888..2916
                     /locus_tag="PKK20_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNV00203.1"
                     /translation="MKIAAISTLGIDGPDNDGYAFGRLISQTTQSIMKHQYTASCSLR
                     LALCGAGWLALAAGLQAQPSFGAHYPIGVEGLKGGSVPPPGLYLRDYNQFYMSDRLKN
                     SGVDDFDLLVYAQAPRLIWITNLKLLGAHYGADVLVPFIYTDLEVRTPDGKFKDSTFG
                     LGDIFVEPITLSWHCQKFDLGVGYGFWAPTGDSDPGLSTAPGKGYWGHMFTLGGTWFP
                     DAQKTWSVSLLNRYEINHESDDYPAASHPYSITAGDVYSLEAGIGKSLSKVVEIGAVG
                     YYQQQTTKDRGTEASNDLDRVFAVGPEISLTFPEASFFASLRYLYEFGAKYRSEGHTL
                     TLTLTKRF"
     gene            2932..3861
                     /gene="trxB"
                     /locus_tag="PKK20_09715"
     CDS             2932..3861
                     /gene="trxB"
                     /locus_tag="PKK20_09715"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNV00204.1"
                     /translation="MEKVVIIGTGCAGLTAAIYTARANLNPLVLTGTMPGGLLTTTST
                     VENYPGFTDGIDGFALMEGMQKQAERFGARIQFGNVESVDLATRPFALAVDGSTVRAD
                     AVIVATGAGHRHLGLESEARLEKKGVTYCATCDGALPMFRNQPLVVVGGGDSACEEAL
                     YLTRFASDVYLIHRRDQLRASKIMADRVLAHAKIKPVWDSVVTEILDVAQDKVTGVRL
                     KQVKTGAETHLPCGGVFVAIGHVPNTHIFQGQLDLDANGYIVPRGGAETSVRGVFVAG
                     DCADHVYRQAITAAGMGCMAAIEAERYLAQSAR"
     gene            3877..4536
                     /locus_tag="PKK20_09720"
     CDS             3877..4536
                     /locus_tag="PKK20_09720"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HNV00205.1"
                     /translation="MKARTAQIERRTTETQIRARLRIDGEGRSVVDTGIPFFDHMLTL
                     FARHALLDLRLHCRGDLAVDAHHTVEDCGLVLGQAFARALGDKKGIRRYGAGFDPRNP
                     LWGEAFVPMDECLARCVIDFGGRPCLVWRGLDRLAYKVARAERAQDMSSAFRFGLARE
                     FFQAFANEARCNLHLELLYGEEPHHVAEALFKAFAKAVDSACQRDPRAPGQLPTTKGR
                     L"
     gene            4589..5215
                     /locus_tag="PKK20_09725"
     CDS             4589..5215
                     /locus_tag="PKK20_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV00206.1"
                     /translation="MSGRPSKPRQKRIEYPSAADEDLVRAAQGGDMVAFEELVGRHRD
                     KVYARAFSMMRNEDEAIDLSQEAWVKGWQRLQQFQGEASFVTWMTRVVINLCLDALRK
                     QKRLRTESIEHMDSESGGVERQMPPLIINPTEGLERQEVRQRIDQAMSQLSYAHRTVL
                     ILHEFEGLEYKEIAKRMECSIGTIMSRLFYARRRLAALLMDLKQERPT"
BASE COUNT         1032 a         1848 c         1622 g          881 t
ORIGIN      
        1 tgtccacata cttcacctcc gcctccggaa accgctcgcg cagcagcccc gccagatgcc
       61 ggacatgatc gcttggctgg gttgtctggg ccgcgattcc gaagcgcgga tgcggacgga
      121 gttgccgcac gtcctggtcg gtcaggatca cgtcgaacgc gtccagatcg cctgtcagac
      181 ctcgcacttc gacatgatcg cgctggccca ctacgaccgg atgatacccc gcgcgcgcca
      241 acgccaggat ggccagatgg gcgtgccgaa ccagcgggca tgtggcctcg atcacatcca
      301 gaccgcgcgc ccgaagcgcc gccttggtcc gattcgaggc cccatgcgcc gtcaccatca
      361 ccgcggacgt ggcgatggat tcgccgaggc ggacggtgcg aatgccgtgg cgtcggagca
      421 ggcccaggac ggtttcgttg tggaccaagt cgccaagaac agtcagcggc tgggagcgtg
      481 cccgatcgag ggcgaggcgg atggcgttgc ggacgccgaa gcacatgccc aggtgatttg
      541 cgcggcgaat tctcatgagt ctttgtgtag caaagtatct gaagggtgga tgaccgcggc
      601 cgacaatgcg ttcaaactcg cttcgcctcc cgcacaatct gctcgagcag gtttatgtac
      661 tgtcgaaatg ccgcccggcc cgccggcgtc agactgcacg tcgtcagccg acggcgtgcg
      721 tagaacgact tggtgatggc cacgtacccg gcctcctgca gcgtgcgcag gtgcacgctc
      781 aggttgccgt cggtcatctg gagcgtgtcc cgaatctccg tgaacgacag ctccgcattt
      841 gccgcaagca gcgtcacgat cggcaggcgg cccttctcgt ggaccacccg gtcgagctgg
      901 agaaacggtt ccgggttcac gcgctcaggg ctttgggttc agtgcaggtg aggtagacgc
      961 cgtagatcag gtgcagcacg ccgaaaaaca agcccatcac tccgtgagcc caaagacacc
     1021 ggcccacttc ccgcaatccc acccactcga ccacgggcag gtgcaccccg gcgaacaagg
     1081 cccagcctcc aagaatcaat acccagccaa accacttgat gccgcgcggc atgaagaacc
     1141 cggccgcgtg caacgcacac ccgtacagca ccgcccacgc catcggcaac tcgcgcatcg
     1201 ccgcggcccg ccacggcccc gcttcctgcg gctcgagcac cagcacccca tacaccatca
     1261 gccccagcac caacccgctg ccggccgccg gcaaacccgc ccgcaccacc cgctgcgtcg
     1321 gcagggacca aaacggttcc gacgccctca gcgcctggcg gcggacaagg acgagggcgg
     1381 cggacatggt cgccagggca accgccagcc agtacacgac aaaccggtgg gccgcggcct
     1441 cctccgcgcc gatctgcgcc cagcccccga ccaccgcagc cgcggtgcca agcgcgccgg
     1501 ccagcaacat catcggcgcc agggcacgcc ggtacagaat cgaccgctcc atcagaatcc
     1561 gaatcacctg gagatgctcg gtcgcaaggt cggattgcat gagagcactt tgcataacaa
     1621 agcttgcgtg tcaagaccta aaccccggaa tcctgcgcca agggcatccc atgtttctcc
     1681 cgctggcatt ctcgtgatcg taatcgtcat cgtcgattgc gacaggcgag aaaggctgaa
     1741 caagatgaga tgcaaggccg caccaagaag gtcggcactg cgtttcaacc ggctccggca
     1801 agaaccggct gaagccggga cacccaacca cgtccgacgc ttcggcgcgg tgttctgtgt
     1861 accgccttca ggcggctcgt ggccaaaatg aaaatcgctg caatctcgac actcggtatt
     1921 gacgggccgg acaatgacgg ctatgccttc gggcgtttga tcagtcaaac aactcagtcg
     1981 atcatgaaac accaatacac tgcctcgtgt tcgttgcgtc tggccctgtg cggggccggc
     2041 tggctggccc tcgccgcggg cctccaggcc caaccctcgt tcggcgccca ctaccccatc
     2101 ggtgtggaag gtctcaaagg cggcagcgtg ccgcccccgg gactctactt gcgcgattac
     2161 aaccagttct acatgtccga ccggctcaag aactccggtg tggacgactt cgatctgctg
     2221 gtctacgcgc aggcgccgcg cctgatctgg atcaccaacc tcaagctgct cggagctcat
     2281 tacggcgccg atgtgcttgt gcccttcatc tacaccgacc tcgaagtgcg gactcccgac
     2341 gggaagttca aggatagcac cttcggcctg ggcgacatct ttgtcgaacc catcaccctc
     2401 tcctggcatt gccagaagtt cgatctgggt gtcggctacg gcttctgggc gcccacgggc
     2461 gactccgacc ccgggctgag caccgccccg ggcaagggct actggggcca catgttcacc
     2521 ctcggcggca cctggttccc cgatgcgcag aagacctggt cggtctcgct gctcaatcgc
     2581 tacgagatca atcatgaaag cgacgattac cccgccgcct cccacccgta ttccatcacc
     2641 gcgggcgacg tctactcgct cgaagcgggc atcggcaaga gcctgagcaa ggtggtcgag
     2701 atcggagccg tcggctatta ccaacaacag accaccaagg accgcggcac cgaggccagc
     2761 aatgatctcg atcgcgtctt cgccgtcgga cccgagatca gcctgacctt ccccgaagcc
     2821 tccttctttg cctccttgcg ctacctgtac gaattcggcg ccaaataccg gtccgaagga
     2881 cacaccctga ccctcaccct caccaagcgg ttctgatcct cgacccgctc tatggaaaaa
     2941 gtggtgatca tcgggacggg ctgcgccggg ctcacggctg cgatctacac cgcgcgcgcc
     3001 aacctgaatc ccctggtcct gaccgggacc atgcccggcg gcctcctgac caccaccagc
     3061 accgtcgaaa actaccccgg cttcacggac gggattgacg gtttcgccct catggagggc
     3121 atgcagaaac aggcggagcg ctttggcgca cgcatccagt tcggcaacgt cgagtctgtg
     3181 gacctggcga cgcgtccgtt tgccctggcg gtggatggct ccaccgtcag ggcggacgcc
     3241 gtgattgtcg cgaccggcgc cgggcacagg catctgggcc tcgagagcga ggcccggctc
     3301 gaaaagaagg gcgtgaccta ctgcgccacc tgcgacggcg ccctccccat gttccgcaac
     3361 cagcccctgg tggtcgtggg aggcggcgac tccgcctgcg aagaggccct gtacctcacg
     3421 cgattcgcct cggacgttta tttaatccat cgacgcgacc agttgcgcgc ctcgaagatc
     3481 atggctgacc gggtgctggc ccacgccaag atcaaaccgg tctgggactc ggtggtgacc
     3541 gagattctcg atgtcgccca ggacaaggtc acgggtgtcc ggctcaaaca ggtcaagacg
     3601 ggggcggaga cccatctgcc gtgcggcggc gtcttcgtcg ccatcggcca cgtgcccaac
     3661 acacacatct tccagggaca gctcgatctg gatgccaacg gctatatcgt tccccggggc
     3721 ggcgccgaga cgagcgtccg gggcgtattc gtggcgggcg attgcgcgga tcatgtctat
     3781 cgccaggcga tcaccgcggc cggcatgggc tgcatggccg caatcgaggc ggagcgctac
     3841 ctggcccaat cggcccggta acctgtgcga tccgccatga aggcgcggac ggcccaaatc
     3901 gagcgacgca ccacggaaac acagattcgt gcccgcctgc ggatcgacgg cgaaggacgg
     3961 tcggttgtcg ataccgggat cccgttcttc gaccacatgc tcactctctt cgcgcggcac
     4021 gcgctgctcg atctgcgact gcactgccgc ggagaccttg ccgtggatgc ccaccacaca
     4081 gtcgaggact gcggcctcgt gctcggccag gcgttcgcac gcgccctcgg cgacaagaaa
     4141 ggcatccgac gctatggcgc cggctttgac ccccgcaatc ctctctgggg cgaggccttc
     4201 gttccgatgg acgaatgcct ggcgcgatgc gtgatcgatt ttggcgggcg cccctgcctg
     4261 gtctggcgcg gattggatcg cctggcctac aaggtcgcgc gcgccgagag ggcgcaggac
     4321 atgtccagcg ccttccggtt cggcctggcc cgggagttct tccaggcctt cgccaacgaa
     4381 gcccgctgca acctccacct cgagctgctc tacggcgagg agccgcacca cgtggccgag
     4441 gcccttttca aagccttcgc caaggccgtc gactccgcct gccagcgcga tccacgcgcc
     4501 ccgggacaac tgccgaccac caagggccgg ctctgaacgg acgccccccg cccatgccac
     4561 gccccatcca cctcgcccct gaatatccat gagcggccgg ccgtccaaac cgcggcagaa
     4621 acgaatcgag tatcccagcg ccgctgacga ggatctggtg cgcgcagcgc aaggcggcga
     4681 catggtggca ttcgaggagc tggtggggcg gcaccgcgac aaggtttatg cccgcgcatt
     4741 cagcatgatg cggaatgagg acgaagcgat cgatctttca caggaagcct gggtgaaggg
     4801 atggcaacgg ctgcagcagt tccagggaga ggccagcttc gtcacctgga tgacccgagt
     4861 tgtcatcaac ctgtgcctcg acgccctgcg caagcagaaa cgcctgcgga ccgagtcgat
     4921 cgagcatatg gacagcgagt cgggcggtgt ggaacggcag atgccgcctc tcatcatcaa
     4981 cccgaccgag gggctggaac gtcaggaagt gcgccaacgc atcgaccagg ccatgagtca
     5041 gttgtcctat gcgcatcgaa cggtgctgat tctccatgaa ttcgaaggcc tcgaatacaa
     5101 ggagatcgcc aaacgcatgg aatgctcgat cggcacaatc atgtcgcggc tcttttacgc
     5161 gcgccgacgc ctggccgctt tgttgatgga tctgaagcag gaacgaccaa catgaaccac
     5221 gaggcagcac tgagaattca ggcctgggtc gatggcgaac tgccctcgcc cgcagaccgc
     5281 gaagtcgccg catgggtgga acgtgatccc gacgccgccg ccctcgccac cgagctgcgt
     5341 catgcccgct ccgccctccg cgagggcgag cccgaataca gtg
//
LOCUS       DAOQRO010000254         5365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15764_length_5365_cov_3.998117, whole genome shotgun sequence.
ACCESSION   DAOQRO010000254 DAOQRO010000000
VERSION     DAOQRO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15764_length_5365_cov_3.998117"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..2956)
                     /locus_tag="PKK20_09730"
     CDS             complement(41..2956)
                     /locus_tag="PKK20_09730"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNV00207.1"
                     /translation="MKTRRLRLLILAAVLAFLHGLDTRAGVVVGNLRCEFRANPLGID
                     CRQPRLAWIMRSDRRAETQTAYHVLAASRPEILAREEGDLWDSGKVLSEDSIHVLYEG
                     EPLRSRQRVWWKVRVWATADEPSEFSEPAWFETALLDAEDWRAQWIQAEPDRAIPDAE
                     MFEDNPAPLLRKEFALEKEIRCARAYVSGLGYYELRLNGERVGDHVLDPGWTSYARRV
                     LYSTYDVSAQLKRGPNALGLMLGNGWFNPLPLRLWNHINLRDTLPHGEPRAILQLVVE
                     FVDGTSQTVVTDETWRACDGPIVRNNVYLGEVYDARREQPGWDTPGFDDSQWTHTLRS
                     TAPKLGSLRAQDAPPIRITTLIKPVNRTEPRPGLFIFDFGQNFAGWARLRVSGPAGTR
                     VRLRYGELLEPDGTLNGMTAVCGQIKGGGKDYRYDGVGQPKTAFQIDEYVLKGDAIDS
                     AAPGIAQNLGDPLTVSLSPTRGGRMSESELRGPATTGRTEVYTPRFTFHGFRYVEVTG
                     YPGTPGLDALEGLRLNSDVPSAGSFECSNDLFNRIQRMVLWTELSNIFSVQSDCPHRE
                     KFGYGGDIVASSEMAMLNFDMARFYAKAAQDLVDDVRPNGGFTETAPFVGISDEGLGD
                     KSGPVGWGTAQPLLLVQLRQYYGERRMIEDQYDAARRWVALLRAKAVEGILDNGISDH
                     ESLAPKPRALTGTAFYYFNARLLARLAGALGRDADAREANALADSIQNAFNRKFLDPD
                     TGRYDIATQACQAFALHYGLVPADRQARALDVLVRDVARHEDHLTTGIFGTKYLLDAL
                     TDAGRADVAYRIVNQRTFPGWGHMLERGATTLWEHWEFSDNTYSHNHPMFGSVSEWFY
                     KALAGIQPAPDAVGFNRILIKPQPIGDLRWVRAAHDCVRGRISSAWEKSADTFTLRLS
                     IPANATALVYIPSADPKRITEDGQPAASAPGIKFLRTDGNTAVFGIGSGDYTFQTPL"
     gene            complement(2946..3083)
                     /locus_tag="PKK20_09735"
     CDS             complement(2946..3083)
                     /locus_tag="PKK20_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNV00208.1"
                     /translation="MARFDGRTFEIINIATSTVSNHLRKIYAKLQDNSRAAAVAKLYE
                     N"
     gene            complement(3143..5107)
                     /locus_tag="PKK20_09740"
     CDS             complement(3143..5107)
                     /locus_tag="PKK20_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00209.1"
                     /translation="MKRTSALRGDRHLPAHVAFGLTLASVLALVLCGPVMPRIEAQSW
                     DFNTGTDTGWQHYTLPAYGQAVYTFPPDDTGGKAYRIYAPPTGNDPGGMGNSRAGSFR
                     PDVSLADRFVVSVDMLEWNDVWPQETGMLFYLSDIGLGTTDGYAATYSSGYRNLYVTR
                     IVDERDSGTVGVLEGILLDTSRAYRLEVSCHDGFTFLFRFFDKADLANPWCSIVCQDA
                     ASSYFSGLCALFVWQRNYPSATEGAEATFDNFQASTPAAGAMPAIVTDLSPQPAGKAT
                     AFYPTVSVSILDRDTYVEPTSITLSLDGMPVPSGSLTIDYVVHKADNPGVQDFGGATV
                     TYPISTVLPWGTRHTNVVSFVDSAGVTRSHTWTWTSAYPYLPASSSLPVGSLGVRGFD
                     VRMVQSANGGVLLANTLERARQQLAVPPLIPIDLAATSIVQVLSWDKGGTPESQPANV
                     PGLCSGTYENIAVESLAYLELKAGVHRFRIHTDDRAGLYSASRFAGADAQVLWENPGN
                     TADTYFEFYVEADGLYPVRSLWEETGGRAYHHLYSVDLNSLSETLINDPTDPDGVVRA
                     WYPMVCRSATSVAGPYTIEPKAVNAINTANVAGVDCSPTVVARMVTGGTFTVPIADGI
                     RFYVLDGPRATRITHLSRNGTNAVIAYQIE"
BASE COUNT          949 a         1567 c         1874 g          975 t
ORIGIN      
        1 tgcaagccgg cgctcgtgcg cccgtggatt cttcgcaaaa tcagagaggg gtctggaagg
       61 tgtagtcgcc ggatccgatc ccgaacacag cggtgttccc gtcggtgcgc aggaacttga
      121 tgcccggggc cgacgcggcc ggctgtccgt cttcggtgat gcgcttggga tcggccgatg
      181 gaatgtacac aagggcggtg gcgttggcgg ggatcgagag acgcaacgtg aatgtgtcgg
      241 cgctcttctc ccaggcactc gagatgcgtc cgcggacgca atcatgggcg gcgcgcaccc
      301 agcgcaggtc gccgatgggc tgaggcttga tgagaatccg gttgaaaccg acggcgtcgg
      361 gggcgggctg gatgccggcg agcgccttgt agaaccactc gctgacggag ccgaacatgg
      421 ggtggttgtg cgaatacgta ttgtcgctga actcccagtg ttcccagagg gtggtcgcgc
      481 ctcgttcgag catgtggccc cagcccggga acgttctctg gttcacaatg cgatacgcga
      541 cgtcggctcg cccggcgtcg gtcagggcat cgagcaggta cttcgtcccg aagataccgg
      601 tggtgaggtg atcctcatgg cgggcgacat cccgcacgag cacatcgagc gcacgggcct
      661 gtcggtcggc ggggacgagt ccgtaatgga gtgcgaaggc ctggcaggcc tgcgtcgcga
      721 tgtcgtaacg ccccgtatct ggatcgagga atttccgatt gaaggcattt tgaatcgagt
      781 cggcgagcgc attggcctcg cgggcgtcgg cgtcgcgccc gagcgcgccg gcgagccggg
      841 cgagcagcct ggcgttgaag tagtagaagg ccgtgccggt cagggcgcgc ggtttgggcg
      901 ccaggctttc atggtcgctg atcccgttgt cgagaatgcc ctcgaccgcc ttcgcgcgca
      961 gcaaggcgac ccatcgacgg gcggcgtcgt actggtcctc gatcatgcgc cgctcgccgt
     1021 agtactgacg gagttggacg aggaggagcg gctgcgccgt tccccagccg acggggccgg
     1081 acttgtcgcc aaggccttcg tcgctgatgc cgacaaaggg cgcggtctcg gtgaagccgc
     1141 cgttggggcg gacgtcatcg acaagatcct gggccgcctt tgcatagaag cgggccatat
     1201 cgaagttgag catggccatc tcgctcgaag cgacaatgtc gcccccgtat ccgaatttct
     1261 cgcgatgtgg gcaatcggat tggacgctga agatgttgct caactcggtc cagaggacca
     1321 tgcgttggat gcgattgaac aagtcgttcg agcactcgaa cgagccggcg ctcgggacat
     1381 cggaattgag gcgcagacct tcgagcgcgt cgaggcccgg cgtgccgggg tagcccgtga
     1441 cctcgacgta tctgaacccg tggaacgtga agcgcggcgt gtatacctcg gtgcggcccg
     1501 tcgtcgcagg gccccgcagc tccgattcag acatgcgtcc gccccgcgtg ggcgagagcg
     1561 acacggtgag cggatcgccc aggttctgcg caatgccggg cgcggccgag tcgatggcgt
     1621 cgcctttcaa gacgtactca tcgatctgga aggcagtctt gggctggccg acgccatcgt
     1681 atcggtaatc ttttccacca cctttgatct gcccacaaac ggcggtcatg ccgttgagcg
     1741 tgccgtccgg ttcgagcaat tcgccgtaac gcagacggac gcgcgtgcca gcggggccgc
     1801 tgacgcgcag acgcgcccac ccggcgaagt tctgtccgaa atcgaaaatg aagagacccg
     1861 gcctcggttc ggtgcgattg accggcttga tgagggtggt gatgcggatg ggcggggcgt
     1921 cctgggcgcg cagagaaccg agcttgggag cggtggagcg gagggtgtgc gtccactgcg
     1981 aatcgtcaaa gccgggtgtg tcccagccgg gctgttcacg ccgggcgtcg tagacttcgc
     2041 ccaggtagac gttgttccgc acgatggggc cgtcgcacgc gcgccaggtc tcgtcggtga
     2101 ccacggtctg ggaggtgccg tcgacaaact cgacgacgag ttggaggatg gcgcgcggtt
     2161 caccgtgcgg gagggtgtcg cgcagattga tgtggttcca gaggcgcagg ggcaggggat
     2221 tgaaccagcc gttgccgagc atcaggccga gtgcgttcgg gccgcgcttg agttgggcgg
     2281 agacgtcata ggtcgagtac aggacgcgcc gggcgtaact ggtccaaccc gggtcgagga
     2341 catggtcgcc gacccgttcc ccattcaggc gcagttcgta gtaaccgagc ccgctgacgt
     2401 aggcccgggc gcagcgaatc tccttctcga gggcgaactc cttgcgcagg agcggcgcgg
     2461 gattgtcctc gaacatctcg gcgtccggaa tggcccggtc gggttcggcc tgaatccatt
     2521 gggcgcgcca atcctcggcg tcgagcaagg cggtctcgaa ccaggccggc tcgctgaact
     2581 ccgacggctc gtcggcggtt gcccagactc ggaccttcca ccagacccgc tggcgtgagc
     2641 gaagcggttc gccttcgtag agaacgtgaa tcgagtcctc tgaaaggacc ttgccgctgt
     2701 cccacaagtc cccttcctcg cgggccagga tctcgggcct cgatgcggcg agcacatggt
     2761 atgcagtttg cgtttcggcg cgacggtccg aacgcatgat ccaggccaaa cgcggctggc
     2821 ggcagtcgat gcccagcggg ttcgcgcgga actcacagcg gaggttcccg acgacgaccc
     2881 ctgcgcgtgt gtccaggcca tgaaggaatg cgaggacggc ggcgaggatc aggaggcgga
     2941 gtcttctagt tttcatagag cttggccacg gcggcggcgc gggaattgtc ctggagtttg
     3001 gcgtagattt tgcggagatg gttgctgacc gtcgaggtgg caatgttgat aatctcgaag
     3061 gtgcggccgt caaaacgggc caatcctgtc ggggtgtcga tccacaggtg gccgtctggt
     3121 attgggagca gtcagggcga ggtcactcga tctgataggc gatgaccgca ttggtcccgt
     3181 tccggctcag atgtgtgata cgggtggcgc gtgggccgtc cagcacgtag aaccgaatcc
     3241 catcggcgat cgggaccgtg aatgttcccc cggtgaccat ccgggccaca accgtcggac
     3301 tgcagtccac gcctgccaca ttcgccgtgt tgatcgcgtt gaccgccttg ggctcgatgg
     3361 tgtagggacc ggctaccgat gtggcggatc gacagaccat cgggtaccag gctcgaacga
     3421 cgccgtccgg gtcggtggga tcgttgatca gcgtctcgga caggctgttg agatcgaccg
     3481 agtagaggtg atgatacgcg cggccgcccg tctcttccca cagggagcgc accggataga
     3541 gaccgtcggc ttcaacgtag aactcgaagt aggtgtctgc ggtgttcccc ggattctccc
     3601 acagcacctg ggcgtcggct ccggcaaagc gcgaggccga gtagaggcca gcgcgatcgt
     3661 cggtatggat gcggaagcga tggacgcccg ccttgagttc gaggtaagcg agcgactcga
     3721 cggcgatgtt ctcatacgtt ccggagcaca agcccggcac gttggccggc tggctttcgg
     3781 gagtgccgcc tttgtcccag ctcaagactt gcacgatgct ggtggctgcc aaatcgatgg
     3841 ggatcagggg cggcacggcg agttgttggc gggctcgttc gagggtgttg gccaggagga
     3901 cgccgccgtt tgcgctttgg accatgcgca catcgaaccc gcgaacgccc aggctgccga
     3961 cgggcaggct gctgctggcg gggaggtagg gataagcgct ggtccaggtc catgtgtggc
     4021 tgcgtgtcac cccggcgctg tccacgaagg acaccacgtt cgtgtgccga gtcccccacg
     4081 gaagcacggt cgagatcggg taggtcacag tcgcgccgcc gaaatcctga actccgggat
     4141 tgtcggcctt gtgtacgaca taatcgattg tcagggaccc gctcgggacc ggcatgccat
     4201 cgagcgagag cgtgatcgag gttggctcga cgtacgtgtc gcgatccaga atgctcacgc
     4261 tcacggtggg atagaacgcc gtcgcctttc cggcaggctg gggcgacaga tcggtcacaa
     4321 tcgcgggcat ggcgcctgcc gctggagtcg atgcctggaa attgtcgaat gtggcctccg
     4381 caccctccgt ggccgacggg taattgcgtt gccagacgaa cagggcgcaa agccccgaga
     4441 agtaactgct tgccgcgtcc tggcacacga tcgaacacca cggattggcg aggtccgcct
     4501 tgtcgaagaa tcggaaaagg aaggtgaaac cgtcgtggca actgacttcc aggcggtagg
     4561 cgcggctggt gtccaggagg ataccctcca acacgcccac agtcccggag tctcgctcgt
     4621 cgacgatgcg ggtgacatac aaattgcggt acccggagga atacgtcgcg gcatagccgt
     4681 ccgttgttcc aaggccgatg tcggagaggt aaaagagcat gcctgtctcc tgcggccaga
     4741 catcgttcca ttcgagcatg tccacactca caacgaaccg atccgcaaga gacacgtccg
     4801 gacgaaagct ccctgcgcgc gagttgccca tgccgcccgg gtcatttccg gtgggtgggg
     4861 catagattcg gtacgccttg ccgcccgtgt cgtcgggcgg gaacgtgtac acagcctggc
     4921 cgtacgcggg cagcgtgtaa tgctgccatc cggtgtctgt gcctgtgtta aagtcccagg
     4981 actgagcctc gatgcggggc atgactggac cgcagagaac cagggccagg acggaggcca
     5041 gggtgaggcc gaaggcaacg tgggcaggca aatgacgatc gccgcggaga gcagacgttc
     5101 ttttcatgga attcctttat cactcgtctc gtgaccgacc gtgggttggg cgcggtctcg
     5161 atatcatgtg tcggaaagtc tggtggggcg gtggtggcga gtcaagacgc cattcttccc
     5221 gaagaacaat gatcagcgcc gtctggcgac ggcggctacg gaggcggagt agccgtcccg
     5281 caactgcagg accagcgagc caaagcccct ccagcccttc ggggcggggc ggcaccgggc
     5341 catctgcgtc gttcctcggt cggtc
//
LOCUS       DAOQRO010000255         5320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15985_length_5320_cov_5.453371, whole genome shotgun sequence.
ACCESSION   DAOQRO010000255 DAOQRO010000000
VERSION     DAOQRO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15985_length_5320_cov_5.453371"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..832)
                     /locus_tag="PKK20_09745"
     CDS             complement(95..832)
                     /locus_tag="PKK20_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNV00210.1"
                     /translation="MSSSSAHLSAFGAASSPTHVAIIMDGNGRWAKERHLPRIEGHRN
                     GVESVRAVVRAAGEVGIKYLTLYAFSVENWNRPKDEVDMLMKYLARYLKSEIGELNRN
                     NVRLEVIGQTYRLPEFVQQQIDRTRAALSKNNGLTLILALSYGGRIEIVEAMRNIARQ
                     VKEGRIEPAEINEQVVAQNLYTRNYPDPDLVIRTSGELRVSNFLLWQISYAEFVVTPT
                     LWPDFRKPQFLAALEEYARRHRRFGGV"
     gene            complement(829..2103)
                     /locus_tag="PKK20_09750"
     CDS             complement(829..2103)
                     /locus_tag="PKK20_09750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNV00211.1"
                     /translation="MANTILIGAQWGDEGKGKIIDVLTEEADIVVRTQGGNNAGHTVF
                     IGAQKYVLHLIPSGILRPRKKCVIANGVVIDPIALVEEIDGLKKLGVKAANNLFISET
                     AHVVLPYHRDIDAQREVRKGQQKIGTTKRGIGPAYADKMARTGLRMIDLIAPDRFEAL
                     LRDRLKENNEVLKALGAKPLSFKSVHASYRAAGDRLRPYVTNTVVMLNEAIRRGQNLL
                     FEGAQGTFLDIDHGTYPFVTSSNTTAGGACTGSGVPPHRMDRVVGVMKAYTTRVGEGP
                     LPTESQDIADMLHAMGREFGATTGRPRRCGWFDAVATRHACMVNGVDDLAITNIDGLD
                     TVQTIRVCIAYRVGGATYDHVPNNAQLLAQCQPVYADFPGWLTPTGKATTWKALPSRT
                     RQYLKALAELSGAQLLIASVGPARDQTIVVRK"
     gene            complement(2184..3743)
                     /locus_tag="PKK20_09755"
     CDS             complement(2184..3743)
                     /locus_tag="PKK20_09755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV00212.1"
                     /translation="MKKPLRALIIEDSEFDAVLLVNHLRLGQYDVAWRRVDTAEAMSR
                     ALAEQPWDIVFSDHQMPQFSAPEALELLQRTGLDLPFIIVSGGIGEATAVALMKAGAH
                     DFLTKGQLGRLVPATERELRDAANRAARRQAEQQMRENEMRYRLLWHYSPDAILMADS
                     AGTIGFVNPAVQSVFGYTPEELLGQPVSLLLGAEANPPAPAEPEVADTDPDQAPMRES
                     VCRHKDGHQITVEVGFSALEWQGRSWRVAFMRDITARCHAERALRARDEEFRVAREIQ
                     RRLFPEQPPVIPGYDIAGSSFPADEAGGDYFDYIEMPEAGLGIVVGDVAGHGMGPALI
                     MAETRAYLRIVAFNHQRPCEALTRANRVLADDLGQSDRFVTVFFARIDAATRTLLYSS
                     AGHPAAYLLDASGGLKRRLARTGPALGLDEDTRYQDAPGVTLDAGDLLVVLTDGVTEA
                     VDAQGNLFGADRVLEVVRSHQHLPAQVIVKALCAATRAFADGGRQTDDVTVLVVKALA
                     DSVVDGRPTAS"
     gene            complement(3697..4179)
                     /locus_tag="PKK20_09760"
     CDS             complement(3697..4179)
                     /locus_tag="PKK20_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00213.1"
                     /translation="MCHAEHVPHEDYGIWGQTLAARIDTDGTMRVLLPSRHIEGGAGT
                     IGSASGPWSQPLRESGFTITAHGGRTVSLTRAACEVFDMEAEVVLRSGTARLTIEREP
                     VRDSLSVLRCEDDACFESAIAKARNCAECPSVRFGSGLSSPAHHEEAAAGVDYRGLGV
                     "
     gene            complement(4847..>5320)
                     /locus_tag="PKK20_09765"
     CDS             complement(4847..>5320)
                     /locus_tag="PKK20_09765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV00214.1"
                     /translation="RVQALLVYTIDRFTREELSDAEKAARRERVVKAIQRLQAGEEFT
                     KVAREVSEDPDIEKNGGEYTTVRDAIAYPELRVALFSLPIGQLSDIITTRAGFYVAKV
                     LERTPAGKVPFDQAEAEIRNALIGQEVQKRLPDFFDGLKKEFEVAYSTNSVSAVR"
BASE COUNT          898 a         1644 c         1787 g          991 t
ORIGIN      
        1 gacgcaccga cgcaccgacg caccgacgca ccgacgcacc gacgcaccga cccccaatct
       61 cccatctccg atcttcgaac tccgaactcc gatctcacac accgccgaac cgccggtgtc
      121 tgcgggcgta ttcttcgaga gcggccagga actggggctt gcggaaatcg ggccagagtg
      181 tgggggtgac cacgaattcg gcgtacgaga tttgccagag gaggaaattg ctgacgcgca
      241 actcgccgct ggtccggatc accaggtccg gatccgggta gttccgggtg tagagattct
      301 gggcgacgac ctgctcgttg atttcggcgg gttcgatcct gccttctttg acttggcggg
      361 cgatattgcg catggcctcg acgatctcga tgcgtccgcc atagctgagg gccaggatga
      421 gcgtgagacc gttgtttttg ctgagagcag cccgggtgcg atcgatttgc tgttgaacga
      481 actcgggcag gcggtaggtc tggccgatga cttcgaggcg cacgttgttg cggttcaatt
      541 cgccgatctc gctcttgaga taccgcgcca ggtacttcat cagcatgtcc acttcgtcct
      601 tgggccggtt ccagttttcg acggagaacg cgtagagagt caggtacttg atgccgactt
      661 cacccgcggc gcgcaccaca gcccgcaccg actcgacgcc gttgcggtgt ccctcgatgc
      721 gcggcaggtg gcgttccttg gcccagcgcc cgttgccgtc catgatgatg gcgacatgcg
      781 tcggcgagga ggccgcgccg aacgcgctga ggtgagccga gctcgagctc atttgcggac
      841 gacaatggtc tggtcgcgcg ccgggccgac cgaggcgatg aggagttggg cgccggacaa
      901 ttcagccagg gctttgaggt actggcgggt cctggacggg agggctttcc aggtcgtggc
      961 ctttccggtg ggggtcagcc agcctgggaa atcggcgtag actggctggc actgggccaa
     1021 gagttgggcg ttgttgggca catgatcgta ggtggcgccg ccaacgcggt aagcgataca
     1081 gacccggatg gtttggacgg tatccaggcc gtcgatgttg gtgatggcaa ggtcgtcgac
     1141 gccgttgacc atgcaggcgt ggcgggtggc aaccgcgtca aaccagccgc aacgccgcgg
     1201 gcggccggtg gtggcgccga actcgcgccc catggcgtgc agcatatcgg cgatgtcctg
     1261 gctctcggtc ggcagcgggc cttcgcctac ccgcgtggtg taggctttca tcacgccgac
     1321 caccctgtcc atgcgatgcg gcggcacgcc cgagccggtg cacgcgcctc cggccgtggt
     1381 gttcgaagag gtgacgaacg ggtaggtgcc gtggtcgatg tccaggaacg tgccttgcgc
     1441 tccctcgaag aggaggttct ggccgcgtcg gatggcttca ttgagcatga cgacggtgtt
     1501 ggtgacgtag ggacgcagcc gatcacccgc cgcgcggtat gaggcgtgga cgctcttaaa
     1561 ggagaggggc ttggccccga gggccttgag cacctcgttg ttctctttga gccggtcgcg
     1621 caaaagggcc tcgaacctgt ccggggcgat caggtcgatc atccgcaagc cggttcgggc
     1681 catcttgtcg gcataagccg gcccgattcc ccgctttgtg gtgccgatct tctgctggcc
     1741 cttgcggacc tcgcgctggg cgtcgatgtc gcggtgatat ggcagaacca catgagcggt
     1801 ctcgctgatg aacaggttgt tcgccgcctt gacccccagc ttcttgaggc cgtcgatctc
     1861 ctcgaccaag gcaatcggat cgatcacgac gccgttggcg atgacgcact tcttgcgcgg
     1921 acgcaggatg cccgacggga tcaggtgcag cacgtatttc tgggcgccga tgaacacggt
     1981 gtggccggcg ttgttgccgc cctgggtccg caccacaatg tcggcctcct cggtcagcac
     2041 gtcgatgatc ttgcctttgc cctcgtcgcc ccattgggcc ccaatcagaa ttgtgtttgc
     2101 catagtcctg tgaattgtgt cgggcagcac gaaaaagacc ccgaaaagcc ttcggggcct
     2161 gcactcgccg gttgcatgca aacctaggaa gcagtgggcc gtccgtcaac gacggaatcc
     2221 gccaatgcct tcactaccag cacggtgaca tcatcggtct gccgcccgcc gtcagcaaac
     2281 gcgcgcgtcg ccgcgcatag ggccttgacg atcacctggg cgggcaggtg ctggtgggac
     2341 cggaccactt cgaggacgcg gtccgctccg aagaggttcc cctgggcgtc gacggcctcg
     2401 gtgacgccgt cggttaacac caccaggaga tctccggcat cgagtgtcac ccccggtgca
     2461 tcctggtacc gggtgtcttc gtccaggcca agagccggac cggtgcgtgc caatcgccgt
     2521 ttcagtccgc cggatgcatc gagaagatag gccgccggat gcccggcgct gctgtagagc
     2581 agggtgcgcg tcgcggcatc gattctcgca aagaacaccg tcacgaatcg gtcgctctgg
     2641 cccagatcgt cggccagcac ccgattggcc ctcgtcaatg cctcgcacgg gcgctggtga
     2701 ttgaacgcca caatgcgcag gtaggcgcgc gtctccgcca taatgagagc cggtcccatg
     2761 ccgtgcccgg cgacatctcc caccacgatg cccagacccg cctcgggcat ctcgatgtag
     2821 tcaaaataat ccccgcccgc ttcgtccgcc gggaacgagg agcctgcaat gtcatagccg
     2881 ggaatgacgg gcggctgctc cgggaagagc cgacgctgaa tctcgcgggc gacgcggaat
     2941 tcctcgtcgc gggcccgcaa ggctctctcg gcatggcagc gcgcggtgat gtcgcgcatg
     3001 aaggcgacgc gccaggaccg gccctgccac tcgagcgcgc tgaatccgac ctcgacagtg
     3061 atctggtgcc catccttgtg acggcagacc gactcccgca tgggcgcctg atccgggtcg
     3121 gtgtccgcga cctccggctc ggccggggcc ggagggttgg cctcggcccc gagcagaagc
     3181 gagaccggtt gcccgagcag ttcttcaggg gtgtagccga acaccgactg gacggcggga
     3241 ttgacgaagc caatggtccc tgcggagtcc gccatcagaa tggcgtcggg cgagtagtgc
     3301 cagagcagac ggtagcgcat ttcgttttcg cgcatctgct gttcggcctg gcgtcgcgca
     3361 gcccggttgg ccgcgtcgcg gagttcgcgt tccgtggcgg gcaccagccg cccgagctgg
     3421 cccttcgtca gaaaatcatg ggcgcccgcc ttcatcaggg ccacggcggt ggcctcgccg
     3481 atcccgcccg agacgatgat gaatggcaag tcgaggccgg tgcgctgcag caactcgagc
     3541 gcctcgggcg cgctgaactg aggcatttga tggtctgaga acacgatgtc ccacggctgt
     3601 tcggccaggg cccgactcat cgcctcagcc gtgtcgaccc gtcgccacgc gacgtcgtac
     3661 tggcccaagc gcaggtggtt gacgagcaac accgcgtcaa actccgagtc ctcgataatc
     3721 aacgcccgca gcggcttctt catggtgcgc cggcgaggat agcccagagc cgaacctaac
     3781 agaagggcat tctgcgcaat tgcgagcttt cgcgatcgcg gactcgaagc acgcatcgtc
     3841 ctcacaccgg aggacgctca agctgtcgcg gacgggctca cgttcaatgg tcaggcgcgc
     3901 cgtgccggag cgcaggacga cctcggcttc catgtcgaaa acctcgcagg cagcgcgcgt
     3961 gagactgact gtgcgcccgc cgtgcgccgt gatggtgaag ccggactcgc gcagcggctg
     4021 ggaccacgga cccgacgcgc tcccgatggt tccggcgccg ccctcgatgt gacgagacgg
     4081 gaggagcacg cgcatcgtgc cgtcggtgtc gatgcgcgcg gcaagtgttt ggccccaaat
     4141 cccgtagtcc tcgtgaggca catgctcggc gtgacacacg gacccgtgct gccacaactc
     4201 ccaggcctcg ggatcgagcg cccgcgcgat gggcgcacgg aagatggcct ggaagttccg
     4261 gttgagcccg accgacgtgc gcgggctaga agcaggtgag acggtacaat gggagttact
     4321 ggaccagcgc gatctgcatt tggtgcggcg cgacgttccg gctccggaag cgcggcaccg
     4381 tgccagtcgt gctgaatgca tcagaacgag ctttgggagc tcatggacca gagtggtacg
     4441 gggcgttcgg tgacatcgtg aacccatgga actgtctggc cttggcgtct tggcgtcttg
     4501 gcgtgaggcc atgaactgtc tggccttggc gtcttggcgt gaggccatgg cgtgtgttga
     4561 gcagacggac ggaacacacc atccgcgttg ccttggatgc atatcattcg tgggattcac
     4621 ggcacggatg ggcgatgcac cgggattagg gccaaagagg gcgaacgagt cgaaccggtt
     4681 tcccggcatc gtggtgcggt gggagccggg agcctgcgtt ctttggacct tgcgttgggg
     4741 cctgggaccg gttttcgtct gccgctggtg gagaagccga atcgacggtc gaagatggcg
     4801 gcgggcgcgg cgtgtggtca cccggaagac atccgcgcca tcgaggtcat ctcaccgcgg
     4861 aaacgctatt ggtgctgtag gccacctcga actccttctt gagaccgtcg aagaaatccg
     4921 gcaggcgctt ctgaacctcc tggccgatca gggcgttgcg gatttcggct tcggcctggt
     4981 cgaagggcac cttgccggct ggcgtgcgtt cgagcacttt ggcgacgtaa aagcccgcgc
     5041 gggtggtgat gatgtcgctg agctgtccga tcgggaggga aaagagggcg acccggagtt
     5101 cggggtaggc gatcgcgtcc cgcacggttg tgtattcgcc gccgttcttt tcaatatccg
     5161 gatcttcaga gacctcccgg gccactttcg tgaactcctc gcccgcttgc agtcgttgaa
     5221 tagctttcac gacccgttcg cgccgcgcgg ccttttccgc atcggacaat tcttcccggg
     5281 tgaagcgatc gatggtgtag acgagcaagg cctggacgcg
//
LOCUS       DAOQRO010000256         5319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_15989_length_5319_cov_4.958397, whole genome shotgun sequence.
ACCESSION   DAOQRO010000256 DAOQRO010000000
VERSION     DAOQRO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_5319_cov_4.958397"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1592
                     /gene="dnaB"
                     /locus_tag="PKK20_09770"
     CDS             201..1592
                     /gene="dnaB"
                     /locus_tag="PKK20_09770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV00215.1"
                     /translation="MRADRLPPHSIEAEMGVLGCALMAPGEALGRCIERFKHGAMVFY
                     DLRHQVIFNALVELLDRQAVIDVVTVGQHLKDCNQLDAMGGFAYLSTLIESVPSAASL
                     DYYADIVREKFVLRRMIQTCTNVITRAYEEQSDVDGLLDEVERDILQVSEDRIEPPSR
                     TIKDLVADSVKTIEDYFHRRGALRGLGTGLADLDKITGGLHKGEMIVIAARPSMGKTS
                     LAMNIVEHVASELRQPVGVFSLEMTAESLVMRMLCSLARVNLRSIREGFMSERDFPKL
                     ITATGKLSGAPLYVDDTGGLSILQLRARARRMWQQFGVKLFVIDYLQLLHSTARRADQ
                     NRQQEISDISSGIKSLAKELNVPVIVLSQLNREVERDKSRKPRLSDLRESGAIEQDAD
                     VVGLLYKADPSNPKKAAGAEDEDEEATEADGVPVNLLIAKQRNGPVGDVFLTFLRSFT
                     RFESASRIPEDVA"
     gene            1606..4026
                     /gene="bamA"
                     /locus_tag="PKK20_09775"
     CDS             1606..4026
                     /gene="bamA"
                     /locus_tag="PKK20_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNV00216.1"
                     /translation="MRCRPRLSLALGAAVLLVANNLALQAQEAPRVSSIEIKHVGPPA
                     ASDGLIRAQIRVKEGDTYKPGSVADDVNSLWRTGYFYNIRVIEEPSDAGLKLIYLVQG
                     NPTLTQINFNGNRRLSEGKLRKKLTSKVGEPLNERKLFADTQEILKRYQKAGLQKTEV
                     KYVPVIDEALGKGTVTFEIVEAPKVKIDDVVFVGAQGFKQSKLRRVIKTRRHWMFSWL
                     TGSGVFKDEQFEDDKDRLAEFYRNEGYIDFQIREVKFEETNPKRMLIRIYTDEGARYQ
                     VGSVDFQGNKLFTASDIQTRAVTREGNKVRRGLSMGPGEVFTPRGLSKDREAVQDFYG
                     SRGYIDAQVSPARVPNTAAGAIDLTYKIDEGRKSYVEKIDIKGNTRTKDRVIRRELAV
                     HPGEVFDMVSVRLSTNRLYGLNYFAKIDAQPEPTEVPDRKNLVIAVEEKNTGDFRVGA
                     GFSSVDELVGFVEVSQGNFDLFKWPYFLGTGAGQKLRARVQMGTRRKDFLLTFIEPWF
                     LNRKLALSTDFYHRELSYYSDLYDTVLSGVRVGLTRTLWNDFWLGSIGYEIQNVGIVD
                     MPREPEDPDRDRVPQEIRAEEGYTLVSKIFGSIAYDTRNSVLLPNRGQRTALESEIAG
                     GPLGGDTDFYKLQLRSARYIRGLAPGHVLELSGRIGVVDNYGSDDLVHLFDRFFLGGM
                     YTLRGYRFREVGPYDSRGREPIGGRTYWFGSAEYSIPLIERLRLAVFYDIGNVYPLSY
                     SFDTLPDNYGPHPYGAYSDNWGVGIRFNIPMLGPLRLDYAWPLTHDNYTGDSGRFQFG
                     VGFTREDY"
     gene            4058..4693
                     /locus_tag="PKK20_09780"
     CDS             4058..4693
                     /locus_tag="PKK20_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV00217.1"
                     /translation="MNLSLTKTLIPSLSALCLGLALTGLTPTAQAQPKVAVINLKTVF
                     DGYWKTKQSDVSIKDRQAEFEKERKKMVEDYQKANDEYRKMSESASDPAVSGDERDRR
                     KKTAEGKLAEIKEIEQNIQQFDRQFRTQISDQIKRMRDNIMREIIDVVTSKAKAGAYN
                     LVLDTAAESVSQTPVILFNSGTPDLTEEVLTELNAKAPPGSLDKANEPKTP"
BASE COUNT         1104 a         1582 c         1656 g          977 t
ORIGIN      
        1 acgttcgggt tacccctttt gttaattatc aacggcttgc gcaggcggtt catgggaatt
       61 ggcaataact tgggattgtc tgccgccggt tctcggtgtc tgcttgtggc atggcagatg
      121 ccgcgaacca agcagccgtc aacagtgcga caccggactt gaaggtggcc caacgtcaac
      181 gttctggctc ggggggggca gtgcgggccg accgattgcc gccgcattcg atcgaggctg
      241 agatgggggt gttggggtgc gcgctgatgg cgccggggga agcgctggga cgttgcatcg
      301 agcggttcaa acacggcgcg atggtgttct acgacctgcg gcaccaggtg atcttcaatg
      361 cgttggtcga gttgctggat cgccaggccg tgatcgatgt cgtcacggtc ggacagcacc
      421 tcaaggactg caaccagctc gatgcgatgg gcggtttcgc ctacctctcg acgctgatcg
      481 agagcgtgcc ctcggcggcg agcctcgact actatgcgga catcgtgcgc gagaagttcg
      541 tgttgcgccg catgatccag acgtgcacga acgtcatcac ccgcgcctac gaggagcaga
      601 gcgacgtcga cgggctgctg gacgaggtgg aacgggacat cctccaggta agcgaggatc
      661 ggatcgagcc cccgtcgcgg acgatcaagg acctggtggc cgattcggtc aagaccatcg
      721 aggactactt ccatcggcgg ggggcgctga gggggctggg gacggggctg gccgacctgg
      781 acaagataac cggtgggttg cataagggcg agatgattgt gattgcggcc cggccgagca
      841 tggggaagac ctcgttggcg atgaacatcg tcgagcatgt ggcgtccgaa ctgcggcagc
      901 ctgttggggt gttcagcctc gagatgacgg ccgagtcgct cgtgatgcgc atgctctgct
      961 ccctggcacg ggtcaatctc aggagcattc gcgagggctt catgtccgag cgcgactttc
     1021 ccaagctgat cacggcgacg gggaagcttt cgggggcccc gctctacgtg gacgacacgg
     1081 gcggtctctc gattttgcag ttgcgggcgc gggcgcgtcg gatgtggcaa caatttgggg
     1141 tcaaactgtt tgtcattgac tatctgcaac tgctccactc tactgcgcgc cgggccgatc
     1201 agaaccgtca gcaggaaatc tccgacattt cgagcgggat caagagtctg gcaaaggaac
     1261 tgaatgttcc cgtcatcgtc ctgagccagt tgaatcgcga agtggaacgt gacaagagcc
     1321 gcaagccccg actgtcggac ttgcgcgaat cgggggcgat cgagcaggat gcggacgtgg
     1381 tggggctgct gtacaaagcg gatccgtcca atcccaagaa ggcggccgga gctgaggatg
     1441 aggacgaaga agcgactgaa gcggacgggg tgccggtgaa cctgttgatc gccaagcagc
     1501 gcaacgggcc ggttggggat gtttttctga cattcctgcg gtccttcacc cggttcgaaa
     1561 gtgcgtcgag aattccggaa gatgtggcct agcgtgacca aagccatgag atgtcggccg
     1621 agattgagcc ttgcgcttgg agcagcggtg ctgcttgtgg cgaacaacct ggcgctccaa
     1681 gcccaggaag ccccgcgggt cagttcgatc gaaatcaaac atgtcggccc cccggccgcc
     1741 agcgacgggc tcatccgcgc tcagattcgg gtcaaggagg gcgacacata caagcccggg
     1801 agcgtcgcgg acgacgtcaa cagcctttgg agaaccggtt atttctacaa catccgcgtc
     1861 atcgaggagc cttcggacgc ggggctcaag cttatctacc tggtccaggg caacccgacg
     1921 ctcacgcaga tcaatttcaa cggcaaccgg aggttgagcg aggggaagct caggaagaag
     1981 ctgacctcga aggtgggcga gcctctcaac gagcgcaagc tcttcgccga cacgcaggag
     2041 atactcaagc gctatcagaa ggccggcctg cagaagacag aggtcaagta cgtgccggtg
     2101 atcgacgagg cgctcggcaa aggcaccgtg accttcgaga tcgtcgaggc gcccaaggtc
     2161 aaaatcgatg acgtggtgtt tgtgggcgcc caggggttca agcagagcaa gctgcgccgg
     2221 gtgatcaaga cccggcgcca ctggatgttc tcctggctga ccggcagcgg cgtgttcaag
     2281 gacgagcagt tcgaggacga caaggatcgg ctggccgagt tctatcgcaa cgaggggtat
     2341 atcgatttcc aaatccgcga ggtgaagttc gaagagacca atccgaagcg gatgctgatc
     2401 cgcatctaca cggacgaagg cgcgcgctac caggtcggct ccgtcgattt ccagggcaac
     2461 aagctcttta cggcctccga catccagacc cgggccgtca cccgcgaggg caacaaggtc
     2521 cgacgcggcc tctcgatggg gcccggcgaa gtgttcacgc ctcgagggtt gtccaaggac
     2581 cgcgaggccg tccaggactt ctacggctcg cgcgggtaca tcgacgccca ggtgtctccg
     2641 gcgcgcgttc ccaacaccgc ggcgggcgcc atcgacctca cgtacaagat tgacgagggc
     2701 aggaagtctt acgtcgagaa gatcgacatc aagggcaaca cccgcacgaa agacagggtc
     2761 atccgacgcg aactcgcggt gcatccgggc gaggtcttcg acatggtgag cgtgaggctg
     2821 agcaccaacc gattgtacgg cctgaattac ttcgccaaga tcgacgccca gcccgagccg
     2881 accgaagtgc ctgaccgcaa gaacctggtg atcgcggtcg aagaaaagaa caccggcgat
     2941 tttcgcgtgg gcgccggctt cagctcggtg gatgaactgg tgggattcgt cgaagtcagc
     3001 caggggaatt tcgacctctt caagtggccg tatttcctcg ggacgggcgc gggacagaaa
     3061 ctccgggctc gggtgcagat gggaactcgg cgcaaggact tcctgctcac gtttatcgag
     3121 ccctggttcc tcaaccgcaa gctggcgctt tcgaccgatt tctaccatcg ggaactgagc
     3181 tactacagcg acctctacga caccgtgctg agtggcgtcc gggtgggttt gacccgcacg
     3241 ctttggaacg acttctggtt ggggagcatc ggctacgaaa tccagaacgt gggcattgtc
     3301 gatatgccca gggagcccga ggatcccgat agagatcgcg tgccgcagga aatccgggcc
     3361 gaggaagggt acaccctggt ctcgaagatt tttggctcga tcgcctacga cacccgcaac
     3421 agcgtgctgc ttcccaaccg cggccagcgc accgcgctcg aatccgagat cgccggcggg
     3481 ccgctgggcg gggacacgga tttctacaag ctgcaactcc gctccgcgcg ctacatccgc
     3541 ggtctggccc cagggcatgt gctcgaactg agcgggcgca tcggtgtcgt cgacaattac
     3601 gggtccgacg atctggtgca tctctttgac cggttcttcc tgggcggcat gtacaccttg
     3661 cgcggctaca ggttccgcga ggtcggcccc tacgattcgc gcggcaggga gccgatcggt
     3721 ggacgcacct actggttcgg ctcggccgaa tacagcattc ctctcattga acgtttgcgc
     3781 cttgcggtat tctatgacat aggcaacgtg tatccgctct cgtacagctt tgacacactg
     3841 cccgacaatt atggaccaca tccatacggc gcctacagcg acaactgggg cgtgggaatc
     3901 cgtttcaata ttcccatgct cggcccgttg cgccttgact acgcctggcc attaacccac
     3961 gacaactaca cgggtgactc gggccggttc cagtttggcg tcggcttcac ccgcgaggat
     4021 tactaatcac ccaaaccgca atccaatgct gcatcgaatg aacctctcgc tcacgaaaac
     4081 gctcatcccc tcccttagcg cactctgcct cggcctcgcc ttgaccggcc tgaccccaac
     4141 cgcccaggcc caacccaaag tcgctgtcat caacctcaag accgtcttcg acggctactg
     4201 gaagaccaaa cagtccgacg tctcgatcaa ggatcgccag gccgagttcg agaaggaacg
     4261 gaagaagatg gtcgaggact accaaaaggc caatgacgag tatcgcaaga tgagcgaaag
     4321 cgccagcgac cccgcggtct cgggcgatga acgcgaccgg cgcaagaaga ccgccgaggg
     4381 caaactggcc gagatcaagg aaatcgagca gaatatccag cagttcgatc ggcagttccg
     4441 cacgcagatc tccgaccaga tcaagcggat gcgcgacaac atcatgcgcg agatcatcga
     4501 tgtggtcacg tcgaaggcaa aagccggcgc ctacaacctg gtcctcgaca ccgcagccga
     4561 aagcgtcagc cagacgcccg tcatcctgtt caattccggc acccccgatt tgaccgagga
     4621 ggtcctcacc gaactcaacg ccaaggcccc tcccggttcc ctcgacaagg caaacgagcc
     4681 gaaaacccca tagcccggtt cgttgacagg ctccgcgctc gacctgatcc aggctggctt
     4741 gacaaccgcg gctctctccc ccccgcgcga ctcggcatca tccgacatca cgcggtgtgt
     4801 gagagccttt tccaacccgc atagcagtcg tctttcggcg tctgaccgcc ctttgcactc
     4861 acggggtatg caatatgcgg atcagaggct ggccccgagt tcccgatagt cctgggtccg
     4921 attcaacgcc tcgaggcgcc gtacccaacc ctcgtcctcg ggccgcacaa aggacccgcc
     4981 cggacaggcc gggccttcgt cggacagacc cgcgcctggt cagactctat gctgactttc
     5041 agccgctctg agtcagcata gacccccctt ttcaggaaca actattggat gcaccggaat
     5101 gaccacggtc ttttcatttg gagcggtgct tgacgattat ctggaaaggg atttggggat
     5161 tgttctggca gcgctcctgc agggccggcc aagaaggctc ggggtgaagg tccgccttga
     5221 gcgcgatcag gcagatgcgc agggccgaga ggttgcagtt gtataaagtg acaggttctt
     5281 aatttgacag tggtttgtcg agatcatcgc acgcctact
//
LOCUS       DAOQRO010000257         5305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16061_length_5305_cov_5.930286, whole genome shotgun sequence.
ACCESSION   DAOQRO010000257 DAOQRO010000000
VERSION     DAOQRO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16061_length_5305_cov_5.930286"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..783
                     /locus_tag="PKK20_09785"
     CDS             181..783
                     /locus_tag="PKK20_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNV00218.1"
                     /translation="MKTLRSPLAQMLVLCALISSSASAAESAKDTWDSLFNGKDLSGW
                     VGVNDTKFDVVEGNLRLVGGMGWLRTEKQYRDFVLEFEWRALVPQYDSGIFIRAGLEG
                     KPWPKDGWQVNLRYDALGGLVKGTRPVVPSETPRLPLNQWVKFRIEVKGKQITLDVDG
                     ERAWASDALDAIEGYLGIQAEEKAFDFRNIRIRPLAPAKP"
     gene            872..1864
                     /locus_tag="PKK20_09790"
     CDS             872..1864
                     /locus_tag="PKK20_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00219.1"
                     /translation="MRPSRRRPAARLRRGPAGAARTHLFPDRSGLELERQSEIGHQWR
                     ASKLKYFLYRRALQQVFDYVQDWNRRTGKRVRCYIPSYSLLNYAHWRIVSPESSPGRL
                     NGCDDYIAQVWTGTSRTPNLFRGKLKERTFETAFFEYGSMQNLVRSTGRTVWYLNDPI
                     EDNPDHDWVDDRTNWESTLVALLLQPDVRQYWIAPWPERVFGGRYHKKGNPQEREPFP
                     PAYAAELQTVFNTLNDLRQTRVRWDCGTEGLGILVSDSLMFQRGASAADPKCWHVEGV
                     AGTPAIVLLHAANPPKTVTLAGQQLDDWSWNPDQRLIWIRFGNTSTPRELSIRF"
     gene            2000..3583
                     /locus_tag="PKK20_09795"
     CDS             2000..3583
                     /locus_tag="PKK20_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNV00220.1"
                     /translation="MRSLSNPGFVRFPQSLSVTLLALVAALWLLPLSGLRADVRLPGL
                     FTDNMVLQQGRPLRIWGWADPQEAVSVQFRKQTVKTTTDAHGRWAVTLRPERAGGPDV
                     MVVTGKQRVEIGNVLVGEVWICSGQSNMEWPLHRSFEPQADIASAENPRIRLFTVPKK
                     KAVDPIDDVPANWALCSPATVPNFSAVGYYFGRALERSRQVPVGLIHTSWGGSPAEVW
                     MSQAALEADAGYQRDILDAYTAQKRDYDQDLARWEQEKVEADKAGRKFEKGRPIEPWR
                     PAELYNGMIAPLVPYGIRGAIWYQGESNAGRAWQYRTLFADMIRNWRRDWGQGDFTFL
                     AVQLAPWDRNKKRTLEAIAAEPVESDWAELREAQMHVAKTLRKVGVAVITDVGDKDDI
                     HPTKKVPVGERLALAARAIAYREKMDGLSPVYKSARFSGGRAVVKFDSVGRGLEARGG
                     ELTGFAIAGPDRQFVWAKAEIQGRNKVVVSSPSVPQPTAVRYGWADYPIVNLFNQCGL
                     PASPFRTDDFPMLTAPKTP"
     gene            3676..4587
                     /locus_tag="PKK20_09800"
     CDS             3676..4587
                     /locus_tag="PKK20_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNV00221.1"
                     /translation="MITRRCFCKRAATAAVTTAGAAVCLPRLSAVPPLDRKGKSQLRL
                     SLAAYSFRDFFNAKEAARRITLDDFIDFCADHGCVGTELTSYYFPKEFANDYLVGLKR
                     HAFLRGLAISGTAVGNNFAQPDGPDLDREIAAVKRWIDHAAVMGAPHIRVFAGAPKGI
                     DLPTAKKQCVRALEECGAYAADKGIWLGLENHGGIVADVAVLLEIVQAVQSPWVGVNL
                     DSGNFYGPGDPYADMARLAPYAVNVQVKVEINRAGKGKEPTDLARVMAILRQANYQGF
                     VALEYESAADPWKAVPEWLERMRAAMA"
     gene            complement(4871..>5305)
                     /locus_tag="PKK20_09805"
     CDS             complement(4871..>5305)
                     /locus_tag="PKK20_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00222.1"
                     /translation="QGLRPPFRPLPVYRMVADLDAVLLIGDQAIEFSRRPHPHRIWDL
                     GAAWFERTGLPFVFAGWALRRGAHTPALRDALRDMGARGVAALPRIVTQRTEFDLEFR
                     REYFRRHVYFELGPDEKRGLCRFMEGLRAHGQAPVFEPRFVS"
BASE COUNT          995 a         1716 c         1682 g          912 t
ORIGIN      
        1 gtctgcacta tgccatccaa accccgtcct gagaagaaaa gcggacattt cttgaaggcc
       61 ctatgtcagg agttctggat tcctcttgag cctccggctt cgcggtggca aaaagcaatg
      121 agaaatgcag gctagttgcc gctggatttt cagcccagcc ctgattaggg tcgcccccca
      181 atgaaaacgc ttcggtcccc tctggcgcaa atgctcgtgc tctgcgccct gatttcgagt
      241 tccgcatcgg cggctgagtc cgccaaggac acctgggatt ccctcttcaa cggcaaagac
      301 ctttcgggct gggtcggtgt caacgacacc aagttcgacg tggtcgaggg caacctgcgg
      361 ctcgtgggcg gcatgggatg gcttcgcacc gaaaagcagt accgcgattt cgttctcgaa
      421 ttcgagtggc gcgccctcgt gccccagtac gacagcggca tcttcatccg cgccgggctc
      481 gaaggcaaac cgtggccgaa ggatggctgg caggtgaact tgcgatacga tgccctgggc
      541 gggctggtga aggggacgcg cccggtcgta ccgtcggaga cacccaggct tccgctcaat
      601 cagtgggtca agttccgtat cgaagtgaag gggaaacaga tcacgctcga tgtcgacggc
      661 gagcgggcct gggcaagcga tgctctcgat gcaatcgagg gttacctcgg catccaggcg
      721 gaagagaaag cgttcgattt ccgtaacatt cgaattcgac ccctggcccc ggccaagccg
      781 taacccgctc cgcggcgtca gtcccgcaac cccgggcatc agaacagatg aggtcttgtg
      841 cagtgcccgg gctggtccgc gccctccgga cgtgcggcct tctcgccgcc gacctgccgc
      901 ccgccttcgc cgaggacccg ctggtgcggc tcgaacacac ctgtttccag accggtcagg
      961 gctggagctc gaacggcaat ctgagatcgg acaccaatgg cgcgcgtcga aactaaagta
     1021 cttcctctat cgccgggcgc tccagcaggt tttcgactac gtccaggatt ggaatcgccg
     1081 aacaggcaag cgagtccgct gctacatccc gtcctacagc ctgctcaact acgcccactg
     1141 gcggatcgtg agcccggagt cgagtccggg ccggcttaac ggttgcgacg actacatcgc
     1201 ccaggtctgg accggcacgt cgcgcacccc gaatctcttt cgcggcaagc tcaaagaacg
     1261 caccttcgag accgccttct tcgaatacgg ctcgatgcag aacctcgttc gctcgacggg
     1321 ccggacggtc tggtatttga acgacccgat cgaggacaat cccgatcacg actgggtgga
     1381 tgatcgcacg aactgggaat ccaccctggt ggccttgttg ctgcagcccg atgtccggca
     1441 gtactggatc gcgccctggc ccgagcgcgt ctttggcgga cgctaccaca agaagggcaa
     1501 tccgcaggag cgcgaaccgt tcccgccagc ctatgccgct gagcttcaaa cggttttcaa
     1561 caccctgaac gatctccggc aaacccgcgt ccgctgggac tgcggcacgg aagggctcgg
     1621 cattctcgtc agcgattcac tcatgtttca gcgcggcgcc tccgccgcgg atccgaagtg
     1681 ctggcatgtg gaaggcgtgg caggcacgcc ggcgatcgtg ttgctgcacg ccgccaaccc
     1741 gccgaaaacc gtcaccctcg ccgggcaaca gttggatgac tggtcttgga atccggacca
     1801 gcgcctaatc tggattcggt tcggcaatac ttcgaccccg cgcgagttgt cgatccggtt
     1861 ctgaccgccc cgcgcagcga tccccctgcc ggcgagtctc ccacggacct cgccgttgac
     1921 atcgaacgct gtgctcaatc tcaaatgcac ggatggtttc tccgttgacg aacgagtcgg
     1981 cttgcggtgg attagtccca tgcgttcctt atcgaatcct ggatttgttc gtttccccca
     2041 atccctgtct gtcacgctgc tggccctcgt ggcggccctt tggctgctac cgctcagcgg
     2101 tctgcgcgcc gacgtccggc tccccgggct cttcaccgac aacatggtgt tgcagcaggg
     2161 gcgtcccctc aggatttggg gctgggccga tccgcaggaa gcggtgagcg tccagttccg
     2221 caaacagacg gtcaagacga ccaccgacgc acacgggcga tgggctgtca cgctgcgccc
     2281 ggagcgggcc ggggggcccg acgtgatggt tgtgaccggc aagcagcggg tcgagattgg
     2341 caatgtgttg gtaggcgagg tctggatttg cagcggtcaa tcgaatatgg agtggcctct
     2401 ccaccgttcg ttcgagccgc aagcggacat cgcctccgct gaaaaccccc gaatccgcct
     2461 gttcactgtg ccgaagaaga aggcggtcga tccgatcgac gacgtgcctg ccaattgggc
     2521 gctgtgctcg cctgccaccg tgcccaactt ctcggctgtc ggctactatt tcgggcgcgc
     2581 actcgaacgg tcccgccagg tgcccgtcgg gctgatccac acctcatggg gggggtcgcc
     2641 ggcggaggtc tggatgagcc aggcggcgct cgaagcggac gcaggctacc aacgggacat
     2701 tctggatgcc tacaccgcgc agaagaggga ctacgaccag gacctggcgc gatgggaaca
     2761 ggaaaaggtc gaggcggaca aggccggtcg gaagttcgag aaaggccgtc cgatagagcc
     2821 gtggcgcccc gccgaactgt acaacggaat gatcgccccc ctcgtcccct acggcatccg
     2881 cggggccatc tggtatcagg gcgagtccaa tgccggccgg gcctggcagt atcgaacctt
     2941 gttcgccgac atgatccgga attggcgccg cgattggggg cagggcgatt tcaccttcct
     3001 cgccgtgcaa ctggcgccat gggaccggaa caagaaacgc accctcgaag cgattgccgc
     3061 tgaaccggtc gagagcgatt gggcggaatt gcgcgaggcc caaatgcacg tcgcgaagac
     3121 gctgcgcaag gtcggcgtcg ccgtcatcac cgatgtgggt gacaaggacg acatccatcc
     3181 gaccaagaaa gtgcccgtgg gcgaacggtt ggcgttggcc gcccgcgcaa tcgcctatcg
     3241 cgagaagatg gacggtctga gccctgtcta caagagcgcg cgcttctcgg gcggacgcgc
     3301 cgtcgtcaag ttcgactcgg tcggccgcgg gctcgaagca cgaggcggcg agctgactgg
     3361 attcgcgatt gcgggaccgg accgccagtt cgtgtgggcc aaggcggaga tccaaggacg
     3421 caataaggtc gttgtaagca gtccgtccgt gccccagccg acagccgtgc gctatggctg
     3481 ggcggattat ccgattgtga acctgttcaa ccaatgcggt ctgccggcct cgccgttccg
     3541 caccgacgac tttccgatgc tcacagcccc caagaccccg tgacgcacaa aggcctctcg
     3601 gacggtcggc agcccgcctg atggcgggcc gccggacccg agacacaccg ccccatcaca
     3661 aacccgagga ctcgcatgat cacacgtcgt tgtttctgca aacgggcggc gaccgccgcc
     3721 gtgacgaccg ccggcgccgc ggtctgcctg ccgcgcttga gcgccgttcc gcctctcgat
     3781 cggaagggca aatcccagtt gcgcctgagc ctggcggcct actcgttccg cgacttcttc
     3841 aacgccaagg aggcggcgcg ccgaatcacc ctggacgact tcatcgattt ctgcgcggat
     3901 cacgggtgtg tgggcactga gttgacgagc tattacttcc ccaaggaatt tgcgaacgat
     3961 tacctggtgg ggctcaagcg ccatgccttc ctccgcggtc tggcgatcag cggcacggcc
     4021 gtgggcaaca acttcgcgca gccggacggc ccggaccttg accgcgaaat cgccgccgtg
     4081 aagcggtgga tcgatcacgc cgccgtgatg ggcgctccgc acatccgcgt gtttgccggt
     4141 gccccgaagg gcattgacct tccgacggcg aaaaagcagt gcgtgcgcgc gctcgaagag
     4201 tgcggcgcat acgcggccga caagggcatc tggctcggcc tcgagaacca cggcggcatt
     4261 gtggcggacg tcgccgtcct actcgagatc gtccaggccg tccagtcccc ctgggtaggt
     4321 gtgaatctcg actcgggcaa cttctatgga ccgggcgatc cctacgccga catggcgcgg
     4381 ctggccccct acgcggtgaa cgtccaggtc aaggtcgaga tcaaccgggc cgggaagggc
     4441 aaggaaccga ctgacctggc ccgtgtgatg gccatcctgc ggcaggcgaa ctaccagggc
     4501 ttcgtcgctc tcgaatacga gtcggccgcc gatccctgga aggccgtgcc cgagtggctc
     4561 gaacgaatgc gcgccgcaat ggcctaaccc gcgcacttct tggcgcggca aagccgcaac
     4621 ccagttgggc cacaactaac actcagggca ggcagggccg gcagggcccc gtcgccgaca
     4681 cacgccgtct tcacttgaca tggcgggggg acgagccgtc cccactccga ccttttcgac
     4741 ttcatgaatt ggcgctgact gacagcctgt gcggcatgtt gacttcttcc aaaaaaaacg
     4801 aagttgacag agagtggtac agcgtcagtg cactgggcgg ccagactccg cacgaggact
     4861 gctatgcggg ttaagaaacg aatcgcggct cgaagaccgg cgcctggccg tgggcgcgca
     4921 ggccttccat gaaccggcac agaccgcgct tctcgtcggg cccaagctcg aagtagacgt
     4981 gccggcgaaa atactcgcgc cggaactcga ggtcgaactc ggtgcgttgc gtgacaatgc
     5041 ggggcaaggc agccaccccc cgcgcgccca tgtcgcgaag ggcatcgcgc agggcggggg
     5101 tgtgcgcgcc gcgtcgcaac gcccagccgg caaacacaaa ggggagaccc gtccgctcga
     5161 accacgccgc gccgagatcc cagatgcggt gcggatgggg gcgtcgggag aactcgattg
     5221 cctgatcgcc gatcaggagc acggcatcca ggtccgcaac catgcggtag acgggcagcg
     5281 gacggaacgg ggggcgcaac ccctg
//
LOCUS       DAOQRO010000258         5300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16086_length_5300_cov_3.924881, whole genome shotgun sequence.
ACCESSION   DAOQRO010000258 DAOQRO010000000
VERSION     DAOQRO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16086_length_5300_cov_3.924881"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1363
                     /locus_tag="PKK20_09810"
     CDS             230..1363
                     /locus_tag="PKK20_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNV00223.1"
                     /translation="MTAKQLQSLARQHGTPLVVVDHNVLRRNYTLFRKHLPRVQVYYA
                     VKANADPAIVRTLYNAGASFDVASLPEFMLVYDLIRHLPVKEQQDFIWDKIIYANPTK
                     PRETLVALDRYRPLVTYDNLNEIRKIRQFAPHAGVVLRLRVPNTGSMVELLSKFGCDP
                     GEAVDLILEAFRLGLVVEGLSFHVGSQCTNFENFVQALNMAAAVMNEARSRGHALKLL
                     DIGGGFPAPYNRHVKPFSLLAARINAEIDRLFPPETQILAEPGRFLVATAATSIARII
                     GKAVRDGKTCYFIDDSVYHTFSGIIFDHCQYPLRALKKGKTSICAVFGQTCDGLDVIS
                     QSEELPELEIDDLVYAEKIGAYSSASSTWFNGFPPAKVVHVNQ"
     gene            complement(1432..3009)
                     /locus_tag="PKK20_09815"
     CDS             complement(1432..3009)
                     /locus_tag="PKK20_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00224.1"
                     /translation="MKTNSHHRPTENDFPKSHKFPSGSPTTLEPAPEGPLSEGRSGAH
                     DSPGCPMRMPRRTFLQRGLGAAGVMAFPTILGRGVLGGEATPPPSERIGVGLIGRGAM
                     GAGHLRRLAGDSSFQLLGVCDVDRVRREAGKSTADDIYASEKAQGTYRGCVAYRDYRE
                     LLAREDLDAVVIATPDHWHTYQAIDAMKAGKDVYCEKPISMTIEEGQRTVEVARRYAR
                     IYQTGTQYRSIPTIRRVCQFVREGGLGKVKSVFALWQNLAGWIGSERCRPYASVLRPA
                     VCGQSYVPMDFALPSEPVPEGLDWDLWVGPAPWRPFNTLYHANPSPGVVPWAFADAFG
                     VTSLTWHLAHSADVIQYALGLERSGPVEVIHPSTGEFPTLTCRYANGTLLHLVDHWGM
                     AKDLYQAVPADARLAGNFGGLFVGERGWVTSMSAGGPVEAGPNSLLEEIELKTREVNI
                     GANDHHANWLAGIRTRQAPSTDEEIGHRSASLGHLANIACWTGRSVKWDPVKEEFPGD
                     DVANRMRWRAPRAPWRM"
     gene            3200..3931
                     /locus_tag="PKK20_09820"
     CDS             3200..3931
                     /locus_tag="PKK20_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00225.1"
                     /translation="MSVSTKTQFNRPIRPGRGIAGFTLVEMLVVIGIIGILMSLLMPS
                     LSRAKAKANQIKCLNHMRQLGVSLHLYAEDYDGQFPPRRTPPNAWPHKLKPFFIDWRI
                     ITCPNDRFGVVGLFTDDLNPKNSFLINGFNDFFMKHLPDADYLKHRNWVWPHGMREDS
                     IPSPSQTILFGEKRRGSFHIHMDLDQGVRGNDFEEIDHARHARGSNFAFGDASVRLLT
                     RNQALYPENLWAVTDEFRYPPAPPK"
     gene            3969..>5300
                     /locus_tag="PKK20_09825"
     CDS             3969..>5300
                     /locus_tag="PKK20_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00226.1"
                     /translation="MSLLVVCCALLCVARPGLGASPSRVVFSELMYHPFGDQDDLQFI
                     EIHNPGDVAVDLSRWAVRGGVDFAFPRGASIPPGGYRVVCRNTAAFRVCHGDSTNVIG
                     TFTGSLSRKGERIELADAQGEVRDAVHYGDRPPWPVAADGHGASLERICAGAASNDPA
                     NWCASGIGAGEAFAGTPGRRNSCDSPVPLPRIVEVCHDRPVPGQPIGVSARVTDDAGV
                     ASVALHWEAWLVDSPIAKGETPLIVDQAASDRARYVGAIPGQPESCLIRFVIEARSVS
                     GAVRLCPSATDLHPTFSCATHINTNTARIPFLKLITFGRIQKPGVARRVQALAEESAR
                     PPWATRMDWSCAAVILPPGETGLLMFDHVRVRRRKGGYKIEFQPDRPWQGLRAVNLIL
                     EGSPRYLLSEPLAFELYRRAGVPSPATDHVRLWLDQHALGCHLVVEQPNRAF"
BASE COUNT          924 a         1732 c         1609 g         1035 t
ORIGIN      
        1 gccgtcgtca gacggcgctg gtgtctgcgt tcgccggagc gagttagcgc ggactcacgt
       61 ccgcggctac gcgacggctg cgggacggct gcgatctccg atggatgtgc cgacgatcca
      121 gttaggaccc tactgcctgt tctttctcaa tgttctttct caatggctgt gaagtgtggc
      181 ttgccatttt ccacgcgggc gaggagtttc cccggacgtc gcgcgcacca tgacagccaa
      241 acaactgcaa agtctcgctc gccagcacgg caccccgctc gtggtggtgg atcataacgt
      301 tctcaggcgc aattacacct tgtttcgcaa gcacttgccc cgagttcagg tctattacgc
      361 cgtcaaggcc aacgccgacc cggccatcgt gcggactctg tataatgccg gggcgagttt
      421 cgacgtggca tcgcttcccg agttcatgct ggtttacgac ctgattcggc atttgcccgt
      481 caaagagcag caggacttca tctgggacaa aatcatctac gcgaacccga ccaagccgcg
      541 ggagacccta gttgcactgg atcggtatcg ccccctggtc acctacgata acctcaacga
      601 gatcaggaag atcagacaat tcgccccaca cgccggtgtc gtgcttcgcc tccgagtgcc
      661 caacacggga tcgatggtcg agttgttatc caagttcgga tgcgatccgg gcgaggcggt
      721 ggacctgata ctcgaggcgt tccggcttgg cctggtggtc gaggggctca gcttccatgt
      781 cggcagccaa tgcaccaatt tcgagaactt cgttcaggcg ctcaacatgg ccgccgcggt
      841 gatgaacgag gcgcggagcc gcggccacgc gctgaaactg ctggacatcg gcggagggtt
      901 tcccgcgccc tacaatcggc acgtcaagcc cttcagcctt ctcgccgcca gaatcaatgc
      961 ggaaatcgac cggctcttcc cgcccgagac ccagattctt gccgaaccgg gacgcttcct
     1021 ggtcgccacc gccgccacct cgatcgcgcg catcatcggc aaggccgtcc gcgacgggaa
     1081 aacctgctat ttcatcgatg acagcgtgta ccacactttt tccgggatca tcttcgacca
     1141 ttgtcagtat ccgctccgtg cccttaagaa ggggaagacc agcatatgcg ccgtgttcgg
     1201 ccagacgtgc gacggcctgg atgtcatctc ccagtctgag gaattgccgg aactcgagat
     1261 cgatgatctg gtctatgccg agaagattgg cgcctacagc agcgcttcgt cgacttggtt
     1321 taacggcttt ccgccggcca aggtcgtgca tgtgaaccag tgaccccggt ctcgaagggc
     1381 cgcacggcgc ggcgcagccg cactcggaag tccgaaggac cggactccac gtcacatccg
     1441 ccacggtgcg cgcggagctc gccagcgcat gcgattagcc acgtcgtctc ccggaaactc
     1501 ttccttgacc ggatcccact tcaccgaacg ccccgtccaa catgcgatgt tggccaggtg
     1561 acccaaagac gcggatcggt ggccgatctc ctcatccgtg ctgggcgcct gacgtgttcg
     1621 gatgccggcc agccagttgg catggtgatc gttggcgccg atgttgacct cgcgtgtttt
     1681 gagttcgatc tcctcgagca gcgaattcgg accggcctcg accggtccgc cggcggacat
     1741 cgacgtcacc catccgcgtt cgccgacgaa gagcccgcca aagttgcctg ccagccgcgc
     1801 gtctgcgggc acggcctgat agaggtcttt ggccatcccc cagtggtcca ccagatggag
     1861 cagcgttccg ttggcgtaac ggcatgtcag cgtcggaaac tcgcccgtcg atggatggat
     1921 cacctcgacc ggtccgctcc gctcgagacc cagtgcgtac tggatcacgt ccgccgaatg
     1981 cgccaggtgc cacgtcagcg aggtcacgcc aaaggcgtcg gcaaaggccc acggcaccac
     2041 gcccggcgac gggttggcgt gatagagggt gttgaacggg cgccacggag cgggccccac
     2101 ccagaggtcc cagtccaatc cctccggcac gggctcggac ggaagcgcga aatccatcgg
     2161 cacatacgat tggccgcaca ccgccggtct cagcaccgag gcgtacggtc ggcatcgttc
     2221 cgatccgatc cagccggcga gattctgcca cagggcgaat accgatttga ctttcccgag
     2281 tccgccttcg cgcacgaact ggcagacccg ccggatggtg gggatggacc ggtactgggt
     2341 gcctgtctgg tagatgcggg catagcggcg cgccacctcg accgttcgct gcccttcctc
     2401 gatggtcatc gagatcggtt tctcgcaata gacatccttg cccgccttca tggcgtcgat
     2461 ggcctggtag gtgtgccaat gatccggtgt cgcgatgacg acagcgtcca gatcctcgcg
     2521 ggccagcaac tcgcgatagt cccgataggc cacgcagccg cgatatgttc cctgagcttt
     2581 ctccgacgca tagatgtcat cggcggtgct ctttccggct tcccggcgca cgcgatcgac
     2641 gtcgcatacg cccagaagct ggaaactcga atccccagcc aggcgtctca gatgacccgc
     2701 ccccatcgcg ccccgcccga tcaggccgac gccaatccgc tcgctcgggg ggggagtcgc
     2761 ctcgccgccc aacacgcctc gacctagaat cgtgggaaac gccatgacac cggcggctcc
     2821 gaggccgcgt tgaaggaatg tgcgccgcgg cattcgcatc ggacagccgg gcgagtcgtg
     2881 cgctccggat cgaccctccg acaatggccc ctccggcgcc ggttcgagtg tggtgggcga
     2941 tccagagggg aatttgtggc tcttcggaaa gtcgttttcc gtcggtcggt ggtgcgaatt
     3001 ggtcttcatc gtctgtgtcc tcgtcccggc atgatatttc cgggtttagg cctaaccacc
     3061 ctgcgcggtt tggtcaagcc gctcctgccg aaatctcgag cggccggcga aacaaaacgt
     3121 ttggttccgg gttctgtcta tacaaacacc gccgggggca cagcggtcgc ggtgccatcg
     3181 cagggtgtcg cctggcatta tgagcgtgtc cacaaagacc cagttcaatc ggcccattcg
     3241 gccggggcga gggattgcag gtttcacatt ggtcgaaatg cttgttgtga tcggcatcat
     3301 cggcattctg atgtcgctcc tcatgccgtc tttgtcccgg gcgaaagcca aggccaacca
     3361 gatcaaatgc ctcaaccaca tgcgccagct cggcgtgtcg ctgcatctct acgccgagga
     3421 ctacgatggg cagttccctc cgcggcgcac gcccccgaac gcgtggccgc acaaactgaa
     3481 gcccttcttc atcgattggc ggatcatcac ctgcccgaac gaccgtttcg gtgttgtggg
     3541 attgttcacc gacgatctga atccaaagaa cagttttctg atcaacggtt tcaacgattt
     3601 tttcatgaag catctcccgg acgcggatta cctcaaacac agaaactggg tctggccgca
     3661 cgggatgcgc gaggacagca tccccagccc cagccagacg atcctcttcg gcgagaaacg
     3721 ccgcggttcc ttccacattc acatggactt ggatcaaggg gtgcgcggca atgatttcga
     3781 ggagattgac catgcccggc atgcgcgcgg ctcgaacttc gcgttcggcg acgcgagtgt
     3841 gcgtctgctc accaggaacc aggccctcta ccccgagaat ctctgggccg tgacggacga
     3901 gttccgctac ccgcccgcgc cgccgaagtg atctggaggg gatacggttc gtgcgcacca
     3961 gcccgctcat gtctctgctc gttgtgtgtt gcgccttgct gtgcgtcgcc cgtcccggtc
     4021 tcggggcgtc cccatctcgt gtggttttta gcgagctcat gtaccacccg ttcggcgacc
     4081 aggacgacct ccagttcatc gaaattcaca accccggcga tgtcgcggtc gatctctccc
     4141 gctgggcggt gcggggcggc gtcgatttcg cctttccgcg cggcgcctcg ataccgccgg
     4201 gcggctaccg ggttgtgtgt cggaacacgg cggccttcag ggtgtgccac ggggattcca
     4261 cgaacgtgat cggcacattc accggctcgc tgagccgaaa aggggagcgc atcgagttgg
     4321 ctgatgccca aggcgaagtc cgcgacgccg ttcactacgg cgaccggcct ccctggccgg
     4381 ttgccgccga tgggcacggt gcgtcgctcg aacggatttg tgccggagcg gcctcgaacg
     4441 acccggccaa ctggtgcgca tccgggatcg gcgccggcga ggcctttgca ggcacgccgg
     4501 gccggcgcaa ttcctgcgat tcacctgtgc ccttgcctcg gattgtcgag gtgtgccacg
     4561 accgccccgt gcccggccaa ccgatcggcg tatcggccag ggtgacagac gatgcgggtg
     4621 tcgcgagtgt cgcgctccac tgggaggcat ggttggttga cagcccgatt gcgaaggggg
     4681 agacccctct gatcgtggac caggcggcct ccgaccgcgc gcgctatgtg ggagccatcc
     4741 ccggacagcc ggagagctgt ctgatccggt ttgtcatcga ggctcgaagc gtctccgggg
     4801 ccgtgcgcct ctgtccatcc gcgaccgatc tgcatcccac attctcctgt gccacgcaca
     4861 tcaacactaa caccgcccgc atcccgttcc tcaagctcat cacgttcggc cgcatccaga
     4921 aaccgggtgt cgcgcggcgg gttcaggcgc tcgccgagga gtccgcccga cctccctggg
     4981 cgacacggat ggactggagt tgcgcggcgg tcattttgcc tcctggagaa accggtttgc
     5041 ttatgttcga ccatgtgcgg gtgcgtcgcc gcaagggcgg gtacaagatc gagttccaac
     5101 ccgatcggcc ctggcagggg ttgcgtgcgg tcaacctgat tctcgaagga tcgccgcgct
     5161 atctgttgtc tgaaccgttg gctttcgaac tctatcgccg cgccggggtg ccgtctccgg
     5221 ccacggatca cgtgcggcta tggttggatc aacacgccct gggctgccac ctggtggtcg
     5281 agcaacccaa ccgcgccttc
//
LOCUS       DAOQRO010000259         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16234_length_5270_cov_4.586385, whole genome shotgun sequence.
ACCESSION   DAOQRO010000259 DAOQRO010000000
VERSION     DAOQRO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16234_length_5270_cov_4.586385"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKK20_09830"
     CDS             <1..223
                     /locus_tag="PKK20_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV00227.1"
                     /translation="RLTPRWSFVGFLDVAGLARDIAEYPASEVVASVGAGIRWRTPIG
                     PVRLEYGYNLNRRHRDRAGSLHFSVGFPF"
     gene            complement(815..1303)
                     /locus_tag="PKK20_09835"
     CDS             complement(815..1303)
                     /locus_tag="PKK20_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV00228.1"
                     /translation="MIELKRIVAADKAAAYSELEAQLRALLAGERDFIANAANTAALL
                     YHTVPGLNWAGFYRLVGEVLLLGPFQGKPACARIPIGSGVCGAAAERRASVRVADCRA
                     FPGHIPCDPASNSELVVPLLQEGRLHGVLDLDSPVRNRFDAADQAGLERLAAVFTAAI
                     DW"
     gene            complement(1300..2991)
                     /gene="pgm"
                     /locus_tag="PKK20_09840"
     CDS             complement(1300..2991)
                     /gene="pgm"
                     /locus_tag="PKK20_09840"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNV00229.1"
                     /translation="MKLHSLAGKPPPTDLLIDVSKLEKAYFEGKPDPADPHQWVSFGT
                     SGHRGTPLNGTFTESHIVAITQAICDYRRLKGINGPLFMGKDTHAVSRPAEQTALEVL
                     AANGVEARIQSGDGFTPTPALSRAILAWNDGGESATADGIGITPSHNPPTDGGFKYNP
                     PNGGPADTDVTTWIQNRANELLRSGNREVRRVSYAAALRQSTTSQYDFVQNYIDGLPS
                     VLDLDAIRDSKVRIGVDPLGGASVAYWQPIAERFGLELTIVNPKIDPTFSFMTVDHDG
                     VIRMDCSSRCAMEGLVRFQKDFEIAFGTDPDADRHGIVTPSHGLMDANQYLAVAIHYL
                     HTRRPAWPLTAAIGKTVVSSAMIDRVAQSVGRRLAEVPVGFKWFAGGLHRGTYCFGGE
                     ESAGASFLRRNGSVWTTDKDGLLLGLLAAEIKARTGLDPAQYFYHLSAILGCPHYRRL
                     DAKATPEQKKLLRQVNADAVILKELAGEPVRAITTRDPENGNPIGGLKVTAPNGWFAA
                     RPSGTENIIKLYAESLESEEHLRRIVKDACDVIGQLSGDMTLGLEDGNSTCRPNA"
     gene            3114..4046
                     /locus_tag="PKK20_09845"
     CDS             3114..4046
                     /locus_tag="PKK20_09845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNV00230.1"
                     /translation="MLCEPLLQIDLPGVAKVKSGKVREIFDLGDALLLVATDRISAFD
                     CIMPNGIPRKGEVLTQLSHFWFERSAGLIPNHLLAGPEAPLPAVLQPHAAALARRSML
                     VKKAKPLAIECVVRGYLAGSGWKEYRARQTVCGIALPAGLTESAELPEPIFTPATKAE
                     SGHDENISFDEAAGIVGPELAAQARAASLALYTNARDYARERGIIIADTKFEFGLFDG
                     QLILIDEVLTPDSSRFWPADQYVAGRSQPSFDKQFVRDYLETLDWNKTSPAPALPPEV
                     VAKTQEKYLEAYERLTGNTLSKSPPAKPEFLHRP"
     gene            4403..>5270
                     /locus_tag="PKK20_09850"
     CDS             4403..>5270
                     /locus_tag="PKK20_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNV00231.1"
                     /translation="MPCSLARVNDMIQSPAPGEREVRFVGDIVRFTLRRRDDQPWPPR
                     WRAFLRTNLGRAAAARSEIIASVERERPPALGSWHDIPLKLEGSRWNCEVPLAEVGCF
                     RAKAYVRDERGWQHWPEGPDVSINVQPDFCRTGNIIYCAFTRMFGPTRTATSTVDPKL
                     DARLEVLDRRGYTVIPPSGKLRDLVRALPHVFGTLGCRILHLLPVNPTPTVYARFGRF
                     GSPYAAGDLLAIDPALVEFDRRTTGLDQFRELAWAVHERGGRLFLDMAINHTGWGSTL
                     QEEHPEWFARDRE"
BASE COUNT          893 a         1668 c         1713 g          996 t
ORIGIN      
        1 gcgactcact ccgcgctggt ctttcgtggg attcctcgac gtcgccggtc tcgcgcgcga
       61 catcgccgag tatcccgcca gcgaggtggt ggcgtccgtc ggagccggca ttcgctggcg
      121 cactcccatc ggcccggtgc ggctcgaata cggctacaac ctcaacaggc gccaccgcga
      181 tcgcgccgga tcgctgcatt tctcggtcgg ctttccgttc tgaccgcgcc agggaccgcg
      241 cgctgaaccg gatcggttct gggattggcg ttgtgggcca ggtcgggcag cctttcgcca
      301 tggctgcgac tggtggcgag tcttccgtcg ggactgacga ggtgcaaaac agaccgcctt
      361 gcccgtcccg ccgatgccgc ccgagtgaga ccgtggtgtc agggggcagg tctgtgatca
      421 ggagcgatgt gccggatgtg gcgacgggct cgccgaacct gtaaccgtcg gttgaacgat
      481 agacttggta tcccgtcgcc gccccgctgc cgccctgagc cgcggggggg aaaccaagaa
      541 acgaggattc ctgccggctg agccatggcg cggggattgg ccggcggctc cggcaggaag
      601 atcaagggag ctgcgtcgaa ctgggcgaag taccgcacga gtccctggta agcggcgcgc
      661 gcgcaccagg tccgcgaagt cttgtgagat gtccgcattg cgagattccc gacaagaaac
      721 tgaagggcgc gcagagcaca acgaagaccg atctgagtcc gcgtgtgagt ttcttgccgt
      781 cgatgcggct tcggaccgag ccgtaacatt gcgatcacca gtcgatcgcg gctgtgaaca
      841 cggcggccaa tcgctccaga cccgcctgat ctgcggcgtc gaagcggttc cggaccgggc
      901 tatcgaggtc gagcaccccg tgcaggcgcc cctcctgaag cagcgggacg accagctccg
      961 agttcgacgc cgggtcgcag gggatgtggc cggggaaggc gcggcaatcg gccacccgga
     1021 ccgacgcgcg ccgttccgcc gccgcgccgc agacgccact gcctatcgga atccgcgcgc
     1081 acgccggttt gccctggaac ggtccgagga gcaggacctc gccgacaagg cggtagaatc
     1141 cggcccagtt caacccgggc accgtgtgat agaggagggc ggcggtgttg gcggcgttgg
     1201 cgatgaagtc ccgttcgcct gccagcagcg cgcgcaactg cgcctccagt tcactgtagg
     1261 cggcggcctt atccgcggcg acgatccgtt tgagttcgat catgcgttcg gtctgcatgt
     1321 actattcccg tcttcaagtc cgagcgtcat gtcgccgctg agttgtccga tgacgtcaca
     1381 ggcgtccttg acgatgcgcc gcaaatgctc ctcgctctcg aggctctcgg cgtagagctt
     1441 gatgatattc tcggttccgg acggccgggc tgcgaaccaa ccgttcggcg cggtcacctt
     1501 cagaccgccg atggggttcc cgttctccgg gtcgcgggtt gtgatagccc ggaccggttc
     1561 gccggcgagt tccttgagga tcaccgcgtc cgcgttcacc tgcctgagca gtttcttctg
     1621 ttccggggtc gccttggcgt cgagtcgtct gtagtgggga cagccgagaa tcgctgaaag
     1681 atggtaaaaa tactgggcag gatccaggcc ggttctcgct ttgatctcgg ccgccaggag
     1741 gccgagaaga agtccgtctt tgtcggtggt ccagaccgag ccgtttcggc gcagaaagct
     1801 ggcgccggcg ctctcttcgc ccccgaagca gtaggtgcca cggtgcaatc cgcccgcaaa
     1861 ccacttgaag ccgaccggga cctccgccag gcggcggcca acgctttggg ccacgcggtc
     1921 gatcatggcg ctgctgacca cggtcttgcc gatggccgca gtcaagggcc aagccggtcg
     1981 gcgggtgtgc aggtaatgga tcgccacggc caggtactga ttggcgtcca tcagaccgtg
     2041 tgagggcgtg acaatgccgt gtcggtcggc gtcgggatcg gtgccgaacg cgatctcgaa
     2101 gtccttctgg aaccgaacca aaccctccat ggcgcatcgg ctcgagcaat ccatccgaat
     2161 gacgccgtcg tgatccaccg tcatgaagct gaaggtggga tcgatcttgg ggttcacgat
     2221 ggtgagctcg agaccgaagc gctcggcaat cggttgccaa taggcaaccg aggcgccgcc
     2281 cagcggatcg accccgatcc gcaccttcga atcccggatg gcatcgaggt ccaggaccga
     2341 gggaagaccg tcgatgtagt tctgaacgaa gtcgtattgg cttgtcgtgg attgcctcaa
     2401 ggcggcggca taggaaaccc gcctgacctc tcggttgccg ctgcgcaaca gctcgttggc
     2461 ccgattctgt atccaggttg tcacgtcggt gtccgccggg ccgccattgg ggggattgta
     2521 cttgaagccg ccatcggtgg gcgggttgtg tgacggggtg atcccgatcc cgtcggcagt
     2581 ggcggattcg ccgccgtcgt tccacgccag aatcgccctg gaaagcgccg gagtaggcgt
     2641 gaagccgtcc ccagactgga tgcgggcctc gacaccgttg gcggccagga cttcgagggc
     2701 cgtctgttcc gccggacgcg agaccgcgtg cgtgtctttg cccatgaaca gtgggccgtt
     2761 gatccccttg agccggcgat agtcacagat cgcctgagtg atggccacga tatggctttc
     2821 ggtgaaggtc ccgttcagcg gcgtgccccg atgcccactc gtcccaaaac tgacccactg
     2881 gtgtgggtcc gctgggtccg gtttgccttc aaagtaggct ttctcgagct tgctgacgtc
     2941 gatgagcagg tcagtcggcg gcggtttgcc ggccagcgaa tgcaacttca tatgccggcg
     3001 gagatcatgg agggatgccg catcggatgc cagcaaagaa aagcaaagcc catccggttt
     3061 gtcacatgac gttctggatt ctctcgaggc gcttcggtag actccgcccc attatgcttt
     3121 gcgaaccact gttgcagatt gatctgcccg gcgttgccaa agtcaaaagc ggaaaagtgc
     3181 gggaaatctt cgatctcggt gacgccctgc tgctcgtcgc caccgaccgc atctccgcct
     3241 tcgattgcat catgcccaac ggcattccgc gcaagggcga agtcctgaca caactgtcgc
     3301 atttttggtt cgaacggtcc gcgggtctca tccccaatca tctcctggcc gggcctgagg
     3361 cgcctctgcc ggcggtcctg cagccccacg ccgcggcgct tgccagacgc agtatgctgg
     3421 tcaagaaagc caaaccgctc gccatcgaat gcgtcgtgcg cggttacctg gccggctcgg
     3481 gctggaagga gtatcgtgcc cgccagaccg tctgcggcat cgcgctcccc gccgggctga
     3541 ccgaatccgc ggagttgccc gaacccatat tcaccccggc gaccaaggcc gagagcggcc
     3601 acgacgagaa catctccttc gatgaagcgg ccggaattgt cggtcctgaa ctggccgcgc
     3661 aggctcgggc cgcgagcttg gccctctaca ccaatgcccg cgattatgcc cgcgagcgag
     3721 ggatcatcat cgccgacacc aagttcgagt tcggtctgtt cgacggacag ttgattctca
     3781 tcgatgaggt gctcacgccc gactcctcgc gcttctggcc ggccgaccag tatgtggcgg
     3841 gtcgcagcca gccgagtttc gacaagcagt tcgtgcgcga ctatctcgag acgctcgatt
     3901 ggaacaagac ttcgcccgcg ccggccctcc ctcccgaggt ggtggcgaag acgcaggaga
     3961 agtacctcga ggcctacgag cggttgacag gtaacacact gtctaaatcc cctccagcaa
     4021 aaccggaatt tttgcacaga ccctaagcaa agcgcatgag tggttcctgg aagcagcgtc
     4081 gtcgttgttg tctaactcat tgggccgtag ttttttgcgg cattcgtcca cgctcaggtc
     4141 ggcgtcgagg tcgcggagcg tgtggagtgc ggcggtttat cgccgctttc cgagggtgct
     4201 tccctgtgca ttgccatata ccacgccaaa gcggtgctga agcacacgca ctccagacgc
     4261 ttcgcgctac cgcacacgtt tgtgcccggg cagcttcagc gaaaggtccg catctcagcc
     4321 gccccaaggt tgacgcgtat gcgcagccgc gggatcggcc ctcccgcagg ggcggcctcg
     4381 gagaacaggg taggttgatg ctatgccgtg cagtctcgcg cgtgtgaacg atatgattca
     4441 gagtcctgct ccaggcgagc gtgaggtgcg gttcgtcggc gacattgtcc gtttcactct
     4501 gcgtcgccgc gatgaccagc cgtggccacc gcgttggcgg gcattcctga gaaccaacct
     4561 cgggcgggcc gccgccgccc gctcggagat catcgcctcg gtcgaacgcg agcgcccgcc
     4621 ggccctgggt tcatggcacg atattccgtt gaagctcgaa gggtcgaggt ggaactgcga
     4681 agttccgctg gcggaggtgg ggtgctttcg ggcgaaggcc tatgtccgcg acgagcgcgg
     4741 gtggcagcac tggccggagg gccccgatgt cagcatcaac gtgcaaccgg atttctgccg
     4801 gaccggcaac atcatctact gcgccttcac gcgcatgttc ggtcccaccc gcaccgcgac
     4861 ttcgacggtc gatccgaagc tggacgcccg ccttgaagtc ctcgatcggc gcggctacac
     4921 ggtcatccca ccctcgggca agctgcggga cctggtccgc gcgctgcccc atgtgttcgg
     4981 cacacttggc tgccggattc tgcacctgtt gcccgtcaac ccgacgccca ccgtgtacgc
     5041 tcgcttcggg cggttcggca gtccgtacgc cgccggcgat ctgctggcga tcgatcccgc
     5101 gcttgttgaa ttcgatcgcc gcacgacggg tttggatcaa tttcgcgagc tggcatgggc
     5161 cgtgcacgag cgcgggggcc ggctgttcct cgacatggcg atcaaccaca ccggctgggg
     5221 gtcgacgctc caggaagagc atccggagtg gtttgcgcgg gatcgggaag
//
LOCUS       DAOQRO010000260         5208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16536_length_5208_cov_5.132350, whole genome shotgun sequence.
ACCESSION   DAOQRO010000260 DAOQRO010000000
VERSION     DAOQRO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16536_length_5208_cov_5.132350"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKK20_09855"
     CDS             complement(<1..634)
                     /locus_tag="PKK20_09855"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNV00232.1"
                     /translation="MRTFTVDCLAVRVHSTQSHLAADAADAVARHLEQALEARPQAAA
                     ILASATSQIQFLDALAGRSEIDWSRVTVFHMDEYLGISADHPASFRRFMRERVESRLR
                     PQVFHSIGGDCLEPLVECDRYAALLRAQPIDLCCLGIGENGHVAFNDPPVADFNDPRL
                     VKLVKLDEPCRRQQVGEGCFPTLESVPQYAFSLTVPALCAARKMVCVVPER"
     gene            complement(756..1544)
                     /gene="nagB"
                     /locus_tag="PKK20_09860"
     CDS             complement(756..1544)
                     /gene="nagB"
                     /locus_tag="PKK20_09860"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNV00233.1"
                     /translation="MELIIRPDADAAADLVAQIMARDLRSNPHLVLGLATGRTMERVY
                     ARLVQFHRDEALDFSLCSTFNLDEYVGLPSSDRHSYRYYMNHHLFLQVNIDLRNTHLP
                     DGTAPDLDLECQRYEQSIRRFNGIDLQLLGLGQAGHIGFNEPLSALRSRTRVKALAPA
                     TLAQNAGLFDDPALMPRRAITMGVGTILESRRCLLLATGEHKADIIAKAVEGPITAMI
                     SATALQLHQRCTVVIDEAAASRLEGADYYRWIFENEPEWEAFRR"
     gene            complement(2046..3371)
                     /locus_tag="PKK20_09865"
     CDS             complement(2046..3371)
                     /locus_tag="PKK20_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNV00234.1"
                     /translation="MKTLVALLAVTVGLPFAPWLRAEAARPNVVLIISDDQAWADYSF
                     MGHPHIATPNLDRLAAQSLTFTRGYTPVPLCRPSLASIATGLYPHRHGVVGNDPDLPD
                     KGVNAMAARGEPRYAAYYGAIIEHFQRRPNLIRDLTSRGYVAFQTGKWWEGDPIKTAG
                     FTHAMTQGEGKGGRHGDAGLTIGREGLDPVYRFIEQAEEKPFFVWYAPMLPHAPHTPP
                     KDLLQKYLARTPSEPVARYWACVEWFDQTCGDLLRHLEDRGLRENTIVLYTADNGWLQ
                     DPANANRFAPRSKLTSYEGGVRTPMMISWPGRVKPRFDRNHLASNLDLWPTLAALLET
                     PVPPGLPGLNLTDAKAVGKRTRLYGEQYAHNIADVDAPARSLQHRWMIDGWWKLIVPD
                     PRQLPAARPELYDLRRDPWEKADLAAEQTRRVKQLGQRLDTWWTPGYGP"
     gene            3470..4183
                     /locus_tag="PKK20_09870"
     CDS             3470..4183
                     /locus_tag="PKK20_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00235.1"
                     /translation="MNFNPQKRLLADRVEERGCQALTLIEVAVILVILAVLALLFLPS
                     LDRAKRKSQRITCANNLKQIGIATRLFATDSGDRVPMSVRTNLDSEVALFPGTVGPSN
                     WVRDPRHFYQAAAHELATPSVVLCPADPRAALETTGSASMVPPRPLSVPWEKSSYFVS
                     ADAGESRPPVLLAGDRNLTLDGQPVSPGWVLFSSKHALGWTDEMHRRMGNVLLSDGSV
                     AGLTGMVHTSVEPNRVLVP"
     gene            complement(4480..5001)
                     /locus_tag="PKK20_09875"
     CDS             complement(4480..5001)
                     /locus_tag="PKK20_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00236.1"
                     /translation="MSRFPPGRQALAFEECPCLGQFGRRQVQLLCIQRVRVRPRYDPA
                     TAHETDPSTPTRTRKTALAAFWGTGRLDRPIRQLAQVVRFNHLDPGLTSQRLDGGCGG
                     PACLGAKPARQVRLSGATPGGHRPYRRAGIGKHGIELRDPVCAQHAVQTRLARQPRTA
                     PRRGPASPAPRFP"
BASE COUNT          930 a         1581 c         1775 g          922 t
ORIGIN      
        1 ggcgctccgg cacgacgcac accatctttc gagccgcgca caaggcgggc acggtgaggc
       61 tgaacgcgta ttgggggacg gattcgaggg ttgggaaaca tccttcgccg acctgctggc
      121 gccggcacgg ctcgtccaat ttcaccagct taaccagtcg aggatcgttg aagtccgcca
      181 cgggtggatc gttgaacgcc acgtgtccgt tctccccgat gcccagacag cacaagtcga
      241 ttggctgggc gcgcagcaga gccgcgtacc ggtcgcactc gaccagcggc tcgaggcaat
      301 cgccacctat ggaatgaaac acctgggggc gaagccggga ttcgacccgt tcccgcatga
      361 atctgcggaa gctggccggg tggtcggcgc tgatgccgag gtattcgtcc atgtgaaaca
      421 cggtgacccg cgaccagtcg atctcggaac gcccggccag cgcgtccagg aactggattt
      481 gtgaagtggc cgacgccagg atcgcggccg cttgcggcct ggcctcgagg gcctgttcga
      541 gatgccgcgc cacggcgtcg gccgcatcgg cggccaaatg gctttgcgtg gagtgaacgc
      601 gcacggcgag gcagtcgact gtgaacgtgc gcatggggag cggagccgtc gtcatagggc
      661 aggggggaaa ggggccggac gcggtcgcgc ggagggatct cgcctcgggg gagtctgggg
      721 cgctgaccag gtgatctgat cggaggtcgg gcgcgtcatc gtcggaacgc ctcccattca
      781 ggctcgtttt cgaaaatcca tcggtagtag tcggcgcctt cgagcctgga ggcggcggcc
      841 tcgtcgatta ccaccgtgca ccgttgatgc agctgaagcg ccgtggccga gatcatggcg
      901 gtgatcgggc cttcgacggc cttggcaata atgtccgcct tgtgttcgcc ggtggccagg
      961 agcaggcagc ggcggctttc gagaatcgtg ccgacaccca tggtgatggc gcgacgcggc
     1021 atcagggccg gatcgtcgaa caggccggca ttctgggcga gagtggccgg cgcaagcgcc
     1081 ttgacacggg tgcgggagcg caaggccgag agcggttcgt tgaacccaat atggccggct
     1141 tggcccagac cgagcagctg gaggtcgatc ccgttgaaac ggcggatgct ctgttcgtaa
     1201 cgctggcact cgagatccag atccggcgcg gtgccgtcgg gaagatgcgt gttgcgcaga
     1261 tcgatgttca cctggaggaa caggtggtgg ttcatgtagt agcggtagga atggcggtcg
     1321 ctgctcggca aaccgacata ctcgtcgagg ttgaatgtcg agcacaggga gaaatccagt
     1381 gcctcatccc gatggaactg caccaggcgg gcgtaaactc gttccatggt ccggccggtc
     1441 gccaggccca ggacaagatg gggattggac cgcagatcgc gcgccatgat ctgcgcaacc
     1501 aaatccgcgg ccgcgtcggc atcggggcgt atgatgagtt ccacgggtca attcaaccac
     1561 gaccggcggg agcacacaac cggaaagcgt gcggaaagcg gtgcatctga tagttgttgg
     1621 tattctatcg cccggtgagg gcaccgggcc tgcgacggat ggaaactccc gctgtaggcc
     1681 gcgtgccctg cctgcgccgc aaagcgcagc ggcctgtctg ccgtgtcggg cacggcacag
     1741 gcaggcgcgg caggcaggcc ccacgcggcg ctggaatttg gacattattc ggtttggact
     1801 tcatcgcaca cttcatcgat ctgtggcgag cagcacgaag agggaaggat gttctccctg
     1861 gacaaactga aggtgtacga tcaagcgctg gccagcgcag acgagttgtc acgcgccgcc
     1921 tcggtctgag gcgaagcctc gctaacccgc atatttcaca ccccgtcgat gcacatgacg
     1981 gtcagacttc gcaagaggac caccatgcgg gttagaaact tcgccgagac cgagtcggag
     2041 cccgttcatg gcccgtaccc tggcgtccac cacgtgtcga gccgctggcc gagttgtttc
     2101 acgcgacgcg tctgttctgc cgccaaatcc gccttctccc atgggtcacg gcgcaagtca
     2161 tagagttcgg ggcgggcggc ggggagttgg cgcgggtcgg gaacgatcaa tttccaccaa
     2221 ccgtcgatca tccagcggtg ctgcaggctg cgggcgggcg catcgacgtc ggcgatgttg
     2281 tgggcgtact gctcgccgta gaggcgggtg cgtttgccga ccgccttggc atccgtgagg
     2341 ttgaggccag gcagaccggg cgggaccggc gtttccagca gggcggccag cgtcggccag
     2401 aggtcgagat tgctggccag atggttgcgg tcaaaccgcg gcttcacgcg gccaggccag
     2461 gaaatcatca tgggcgtgcg caccccgccc tcgtaggagg tgagcttcga gcgcggcgca
     2521 aagcggttgg cgttggcagg gtcctggagc cagccgttgt cggctgtgta caaaacgatt
     2581 gtgttttcgc gcaggcctcg gtcctcgaga tgacggagca ggtccccgca ggtttggtcg
     2641 aaccactcga cgcaggccca gtagcgggcc acgggctcgc tcggcgtccg ggcgaggtac
     2701 ttctgcagca aatcctttgg cggcgtgtgc ggcgcgtgtg gtaacatcgg ggcgtaccag
     2761 acaaagaacg gcttctcctc ggcctgttcg ataaagcgat agaccggatc gagcccttca
     2821 cgacctatgg tcaaaccggc atcgccgtgt cggccaccct tgccttcgcc ttgggtcatg
     2881 gcgtgggtga agccggcggt cttgatggga tcgccttccc accacttgcc ggtttggaac
     2941 gcgacatagc cgcgggaggt gaggtcgcgt atgaggttgg gccgcctctg gaagtgctcg
     3001 atgatggcgc cgtaataagc ggcgtatcgc ggttcgccac gcgccgccat cgcattcacg
     3061 cctttgtccg gcaggtcggg atcgttgccg acgacaccgt ggcggtgggg gtacaagccg
     3121 gtggcgatgc tggccagcga cgggcgacaa agcggcacgg gtgtgtaacc gcgagtgaag
     3181 gtcagcgact gagcggcaag gcgatccagg ttgggcgtcg cgatgtgcgg gtgccccatg
     3241 aacgaataat ccgcccaggc ctggtcgtcc gagatgatca ggacgacatt cggccgcgca
     3301 gcctcggcgc ggagccatgg cgcgaacggc aggccgacgg tgacggcgag caaagcaacg
     3361 agagtcttca tgacatcggt ctcggcggga gaatccaagg aaatggcggc cctgtcgagg
     3421 cacaacgcgg cattgaacct gggccggctt gcgctgtagg gtttgagcca tgaacttcaa
     3481 ccctcagaag cgcctcttgg cggaccgcgt cgaggagaga ggctgccaag cgctcacgct
     3541 cattgaagtg gccgtcattc tggtcattct ggctgtcctt gctctcctct tcctgcccag
     3601 cttggatagg gccaagcgaa agtcccaacg gatcacctgc gccaacaatc tcaagcagat
     3661 cggcatcgcc acccgcttgt tcgccacgga ttcgggtgat cgagtcccca tgtcggtccg
     3721 cacgaatctc gactctgagg tcgccctgtt tcccggcacc gttggcccct cgaactgggt
     3781 cagagacccg cgccatttct accaggccgc ggctcacgag ctagccaccc ctagcgtggt
     3841 gttgtgtcca gccgacccgc gcgcggctct cgaaaccacc ggcagtgcgt cgatggttcc
     3901 tccgaggccg ctgtccgtgc cctgggagaa gagcagttac ttcgtgagcg cggatgccgg
     3961 cgaaagccgt ccgccagtgc tcctggctgg cgaccgcaac ctgaccctgg acgggcaacc
     4021 ggtgtccccc gggtgggtcc tcttcagctc gaagcacgcg ctgggttgga ccgacgagat
     4081 gcaccgacgc atgggcaacg tgctgctctc cgacgggagt gttgcgggac tcactggcat
     4141 ggtccatacc agcgtcgagc cgaaccgcgt gctcgtgccg tagggaggga cgaacaccat
     4201 gatgaaatca ctatcgagcc tgctgccggt ggcagtctgg ctggccgcct ccttgtttcc
     4261 atcgcatgcg gagtctgaat tcgatccggc tgggaacccc gcggtcgctg cctagcctag
     4321 ccggtacggg cctttccgga acgactcctc gaaacccctt cccaaacacg cgtactcgcc
     4381 gttcgataag ctgaacgcct cgcgacccta ccagccggcg tccgggcggg actaccggga
     4441 cggcgagttg gtcgtcaagt tcgcgccgga cgtggcggtt tacgggaagc gcggagccgg
     4501 actggctggg ccgcggcgcg gtgcggtgcg gggctgacgc gccaggcgag tctgaaccgc
     4561 gtgttgggcg cacacgggat cacgcaactc gatgccgtgt ttcccaatgc cagcccgccg
     4621 gtacggacga tgtccgccgg gagtcgcacc gctcaaccgg acctgacgcg ctggtttcgc
     4681 gccaagacaa gcggggccac cgcacccgcc gtcgaggcgt tgactggtca acccgggatc
     4741 gagatggttg aaccggacta cttgcgcaag ctggcggatg ggccggtcaa gccgtcccgt
     4801 cccccagaag gcggccaggg cggtctttcg agttcgggtg ggagtcgaag ggtcggtctc
     4861 atgggcggtg gcgggatcat acctgggcct gacacggacc cgttgtatgc acagcaactg
     4921 cacctggcgg cggccaaact gcccgaggca tgggcattcc tcgaaagcca gggcctgccg
     4981 cccggggggg aaccgcgaca tcgtggtggc cgtgatcgac actggcgtgg attacacgca
     5041 ccccgaccta gtgtagtgtc tcataaataa ctggatctcc acggcagttc gaacacgcgg
     5101 tgttcgcacc gatgtccatt cccagcaact cgcgaaaaga ggtgctacta tgaaaaggtc
     5161 ttccttctgc ggcactgctg aactccatcc tgggcgtctg tgggtaca
//
LOCUS       DAOQRO010000261         5196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16601_length_5196_cov_5.240226, whole genome shotgun sequence.
ACCESSION   DAOQRO010000261 DAOQRO010000000
VERSION     DAOQRO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16601_length_5196_cov_5.240226"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1600
                     /gene="rho"
                     /locus_tag="PKK20_09880"
     CDS             176..1600
                     /gene="rho"
                     /locus_tag="PKK20_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNV00237.1"
                     /translation="MTDDAIRDGMRPEPTEESAPAAPSAVAPETAGESPVEAAPARPA
                     PSARPALPSGTTINIAKLQSMSMGELTHMAKEWGIENFGTMKKHEVIFEILKKNAERN
                     GVLFAEGVLEILSEGFGFLRSQSFNYLPCPEDVYVSPSQIRRFDLQTGDIVAGQIRPP
                     KEKEKFFALLKVEAVAHEDPDSAKEKTHFDNLTPLFPNKRVILETTSDELSTRVVDLV
                     CPIGKGTRGLIVAPPRTGKTVLMQKLANAILKNNPEIYLIILLIDERPEEVTDMERTC
                     KGAEVISSTFDEPPERHVQVAEMVIEKARRMTEHKKDVVILLDSITRLARAYNTVQPH
                     SGKILSGGVDANALHKPKRFFGAARNIEEGGSLTIMATALVDTGSRMDEVIFEEFKGT
                     GNMEVHLDRALVDRRIFPSINIERSGTRKEELLYHPDELSRIVLLRRALTGVPPVEAM
                     ELLLGKLKKTQSNIMFLLSMANVS"
     gene            1824..4568
                     /locus_tag="PKK20_09885"
     CDS             1824..4568
                     /locus_tag="PKK20_09885"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNV00238.1"
                     /translation="MNSRHSGCGLPPMRWGGLRAALFLTALLFGAGSMWSVADPAAPA
                     GRLDSRALEFRLAPSGAYEILDRRGQVTWRSNPVHDRFGLVTCAANGEQRTVDLAQPE
                     VVRAGSTLRAVFRPVPDQADATLTVSISLVDGGSALGFSYSADPRLGIESVRLLDDAF
                     GVSEHEGGHMLVPVREGLLIPSDTGLAFTQNFDTYAYEGCHLTMLGVGKRGAMVLVDW
                     EDPYVAAEVKSVLPSENPALNTQTLFASLVLRQSAQSFRVRFLGAADHVALARAYQQV
                     ARERGWVVPWAEKLKRHPERARLFGAINFKLWSALDRQMDEASTREERSHVNWTFAEA
                     GAVAAHLKHDLELERVLFLMGGWIHRGYDNQHPDILPAAPECGGNDGLAECARRVREL
                     GYLFGLHDNYQDMYRDAPSWNEDYLMRNADGTVVRGGRWAGGRAYLTCSQKAIELARR
                     DQNLPAVKRLTCADVYFIDTTYAAGLFECHDPRHPLKRGDDLRWKQEISDYARGLFGI
                     FGSECGREWALPHSDFFEGLTGVSGGYYHNADLLKKTGGRVVPLFELVYRDGIALYGK
                     YGYDIRRAGEYVLHHVAIGRPLNHHNVPPHLYWQNWTPPANELAVEPCRPELEELDEI
                     EPRAVRLSYQWIVRETPKRDWRVFVHFTDSDGKQYQENDHAPRVPTSKWLPGRIESAA
                     FTIRLSERLRGALDVRVGLWDPATGERARLHGSHDGSHRYVVGRLWLGEERVELLPPV
                     YRASDADAEAFVRGDNGWTAGLHPFDRFVKNTYKILSPLNALTSRAPMTGHAFLTQDG
                     LVQRSVFGQGRTAVTVIANFGAAEFAAESELGGKVRLPRYGFLVESRDFITFHALSWN
                     GIDYDGPVLFTMRGESGRALARAPGVRVYHGFGDSRLRWRDTLFEIEKERVVGNLGRD
                     "
     gene            4576..>5196
                     /locus_tag="PKK20_09890"
     CDS             4576..>5196
                     /locus_tag="PKK20_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNV00239.1"
                     /translation="MRAHSFAWWSALVYAGLCWMSPTPMAAEQVRLLVVHGGHEFETN
                     QFLQVFRDNTNVTFEVAQHPAAHARWQAEPAKEWDVLVLYDFWQEIGDEAKADLVARL
                     KEGKGLVVLHHALANYQKWPEFERIVGGKFYLEKQTVNNVEKPQSTYKHDVEFTVRIA
                     NNQHPVTQGLTNFVIHDEVYGGFDVSSQTHALLTTEEPVTFKNDRWD"
BASE COUNT          977 a         1659 c         1623 g          937 t
ORIGIN      
        1 ccgggttcac gatcaatgga ctgtcaactc attgccaact gattagatta tgccacgccc
       61 aaagaagtcc gaacggcctc gccccaaacc gatgcgtcgc ggcaaaacga ccgccgcttc
      121 gaagtcgaat gcccccgagc tcgcgctgga gacaccgtct gcgccgcccg accgaatgac
      181 tgacgacgcc atacgcgatg gaatgcgacc cgagccgacc gaggaatcgg ccccggcggc
      241 gccctcggca gttgcgcccg agacggccgg cgaatcgccg gtcgaagccg cacctgcgcg
      301 gccagcgcct tccgcccgcc ccgccttgcc gagcgggacg acgatcaaca tcgccaagct
      361 gcagtcgatg tcgatgggcg aactgacgca catggccaag gaatggggca tcgagaactt
      421 cggcacgatg aagaagcacg aagtcatctt cgagattctc aagaagaacg ccgaacgcaa
      481 cggcgtcctc ttcgcagaag gcgtgctcga aatcttgagc gagggtttcg gattcctccg
      541 ctcccagtcg ttcaattacc tgccgtgccc ggaagacgtc tacgtttccc catcccaaat
      601 ccgccgcttc gatctccaga caggcgatat cgtggccggc cagatccggc cgcccaagga
      661 gaaggagaag ttcttcgcgc tcctcaaggt cgaggccgtg gcccacgagg atcccgacag
      721 cgcgaaggag aagacgcact tcgacaacct gaccccgctc ttcccgaaca agcgggtaat
      781 tctcgaaacg acctctgacg agctctcgac gcgcgtcgtc gacctggtct gtcccattgg
      841 caagggcacc cgtggcctga tcgtggcgcc gccgcgcacg ggcaagaccg ttttgatgca
      901 gaagctggcc aacgccatcc tcaagaataa tcccgagatc tacctcatca tcctgttgat
      961 cgacgagcgg cccgaggagg tcaccgacat ggagcggacc tgcaaaggcg ccgaggtgat
     1021 cagttccacg ttcgatgagc cccccgagcg ccatgtgcag gtggccgaga tggtcatcga
     1081 gaaggcccgc cgcatgaccg agcacaaaaa ggatgtcgtc atcctgctcg attcgatcac
     1141 tcgcctggcc cgcgcttaca acaccgtcca accccactcg ggcaagatac tctccggggg
     1201 cgtcgacgcc aacgccctcc acaaacccaa acggttcttc ggcgccgcac gcaacatcga
     1261 ggaaggcggg tccctgacca tcatggccac cgccctggtc gacaccgggt cgcgcatgga
     1321 cgaggtcatc ttcgaggagt tcaagggcac cggcaatatg gaggtgcatc tcgaccgggc
     1381 tctcgtcgac cgtcgcatct tcccgtcgat caacatcgag cggtcgggca cccgcaagga
     1441 ggaattgctc tatcaccccg atgaactctc gcgcattgtg cttctgcgcc gcgccttgac
     1501 cggcgttccg cccgtcgagg cgatggaact gctccttggc aagctcaaga agacccagag
     1561 caacatcatg ttcctcctct cgatggcgaa cgtgagttga ccccgctctc cgaggatggt
     1621 cttccgtgcc ccatctccat gaaccgcctg cgcaagccgt tgataattaa caaaaggggc
     1681 aacccgaacg tccaacgcgc aacgttcaac atccaacttt taacgaaccg cttcgttgaa
     1741 cgttgagcgt tggaagttgg gcgttgaacg ttccccggtt catgggtcca atgcgcactc
     1801 aaccgttgaa tgtcaaaact cctatgaact ccagacactc aggatgcggt cttccgccaa
     1861 tgcgatgggg cggcctgcgc gcggccttgt tcctcacagc gcttcttttc ggcgccggtt
     1921 cgatgtggag cgtcgcggac ccggctgccc cggccggcag actcgatagc cgcgcgctcg
     1981 aattccggct ggcgccttcc ggcgcctacg aaatcctcga tcgtcgcggc caggtcacct
     2041 ggcggtcgaa tccggttcac gaccggtttg gtctcgtgac atgcgcggcc aacggcgaac
     2101 agcgaacggt cgatcttgcg cagccggagg ttgttcgagc cggatcgacc ttgcgcgccg
     2161 tgtttcgccc ggtgccggat caggccgatg ctactctgac ggtttctatc tccctggtgg
     2221 atggcggatc ggcgctcggt ttcagttact cggccgaccc gcggcttggc atcgagagcg
     2281 tacggttgct cgatgatgcg ttcggggtga gcgagcacga gggggggcac atgctcgttc
     2341 cggtgcgcga gggtcttctg attccgtccg acactggact ggcgttcacc cagaactttg
     2401 acacctacgc gtatgaaggg tgccatctga caatgctggg cgtggggaag cgcggggcaa
     2461 tggtcctggt ggattgggaa gatccgtacg tggcggccga ggtgaagagc gtgctgccga
     2521 gcgagaatcc cgctctgaac acccagacgc ttttcgcctc ccttgtcctg cggcaaagcg
     2581 cccagtcgtt ccgcgtccgg tttcttggcg cggccgatca tgtggcgctg gccagggcct
     2641 atcaacaggt cgcgcgggag cgcggctggg tcgttccctg ggcggagaag ctcaagcggc
     2701 atccagaacg cgcgcgcctc ttcggcgcca tcaacttcaa gttatggagc gcgttggacc
     2761 ggcagatgga cgaagccagc acgcgggagg agcggtcgca tgtgaactgg acctttgccg
     2821 aggcgggtgc ggtggccgcc catctcaagc acgatctcga actcgagcgg gtgcttttcc
     2881 tcatgggcgg ctggattcat cgcggctatg acaaccagca tccggacatt ctgcccgcgg
     2941 ccccggaatg cggggggaat gacgggctgg ccgaatgcgc ccggcgtgtc cgggagcttg
     3001 gctacctgtt cggcctgcac gacaactacc aggacatgta ccgcgatgcg ccttcgtgga
     3061 atgaggacta tctgatgcga aacgccgacg gaaccgtcgt ccggggcggg cgttgggcgg
     3121 gcggacgcgc ctacctgacc tgctcgcaga aggcgatcga gctggcccgg cgagatcaga
     3181 atctgccggc agtcaagcgc ctgacctgcg ccgacgtgta cttcatcgac accacctatg
     3241 cggccggtct gttcgagtgc cacgatccga ggcatccgct gaaacgcgga gatgaccttc
     3301 ggtggaaaca ggagatctcg gactacgcgc gcggcctctt cggcattttc ggcagcgaat
     3361 gcgggcgcga gtgggctctg ccgcacagcg atttcttcga aggcctgacg ggtgtcagcg
     3421 gcgggtatta tcacaacgca gatctcctca agaagaccgg cggacgcgtc gttccgctgt
     3481 tcgagcttgt ctaccgcgac gggatcgcac tctacggcaa gtacggctac gacatccggc
     3541 gcgccggaga gtatgtgctc catcacgtgg cgatcggccg cccgctcaat caccacaacg
     3601 tgccaccgca cctctactgg cagaattgga ccccacccgc caacgaactg gctgtcgagc
     3661 cgtgccgtcc ggaactcgaa gaactcgacg agatcgagcc gcgcgcggtg cgactcagct
     3721 accaatggat cgtgcgcgag acgcccaagc gcgattggcg ggtctttgtc cacttcaccg
     3781 attccgacgg caagcagtac caggaaaacg atcatgcgcc gcgcgtgccg acctcgaagt
     3841 ggctgcctgg acggatcgag tccgccgcgt tcaccatccg gctttccgag cggctgcgcg
     3901 gtgccctcga tgtgagggtc gggctctggg atccggccac gggcgagcgc gcccggttgc
     3961 acgggagcca cgacggctcg caccggtacg tggttgggcg gctttggctg ggggaggagc
     4021 gcgtcgagtt gctgccgccg gtttaccgcg cctcggacgc cgatgccgag gctttcgtgc
     4081 gcggcgacaa cgggtggacg gccggcctgc atccattcga ccggttcgtg aaaaacacct
     4141 ataagatcct ctctccgctc aacgcattga cctcgcgcgc cccgatgacc ggtcacgcct
     4201 tcctcacgca agacggcctg gtgcagcgca gcgtgttcgg ccagggccgc actgcggtga
     4261 cggtgatcgc caattttggc gccgccgagt tcgccgccga gtcggagctg ggtggcaagg
     4321 tgcggctgcc ccgttacggg ttcctggtcg agtcgcgcga cttcatcaca ttccacgcct
     4381 tgagttggaa tgggatcgac tacgacgggc cggtcctgtt cacaatgcgg ggcgaatccg
     4441 ggcgcgcgct ggccagggcg ccgggcgtgc gcgtttacca cggctttggc gactcgcgcc
     4501 tgcggtggcg cgacacgctc ttcgagattg aaaaggagcg cgtggtcggt aacctaggcc
     4561 gcgactagat ttgttatgcg cgcacattcg ttcgcctggt ggtctgctct cgtgtatgcg
     4621 ggtctctgtt ggatgtctcc aacaccgatg gccgccgagc aggtccggct tctcgttgtc
     4681 cacggcggac acgagttcga gaccaaccag tttctccagg ttttccgaga caacacgaat
     4741 gtgacattcg aggtggccca gcatccggcg gcgcacgccc gctggcaggc cgagccggcc
     4801 aaagagtggg atgtgctggt tctgtacgac ttctggcagg agatcggcga cgaggccaag
     4861 gccgacctcg tcgcccggct caaggaaggc aagggcctgg tcgtcctgca ccatgccttg
     4921 gccaattacc agaagtggcc ggagtttgaa cggatcgtcg gcggcaagtt ttacctcgag
     4981 aagcagaccg taaacaacgt cgagaagccc cagtccacct acaaacacga cgtcgagttc
     5041 acggtgcgga ttgccaacaa ccagcatccg gtgacccagg gactgacgaa tttcgtgatc
     5101 cacgacgaag tgtacggggg gttcgatgtc agttcgcaga cgcacgccct gctcaccacc
     5161 gaggagccgg tcaccttcaa gaacgaccgc tgggac
//
LOCUS       DAOQRO010000262         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16607_length_5195_cov_4.924708, whole genome shotgun sequence.
ACCESSION   DAOQRO010000262 DAOQRO010000000
VERSION     DAOQRO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16607_length_5195_cov_4.924708"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PKK20_09895"
     CDS             complement(<1..1375)
                     /locus_tag="PKK20_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00240.1"
                     /translation="MTCRSVSLIAIAAAAWLSALLDLDPAVGRAQEIRINELMSSNGA
                     NPADENGDFPDWFELFNPTSDAVSLDGWGVSDDVRRPFKWVFRNAVLGAGEHLLLFAS
                     GKDRQPASVDSMGPDTISGLRLWLRADAVNPNSSTQVRRDGTTVYVRQWRDQSGAGNH
                     AVQTTGANQPTWLAAGPTGTPALRFDGVDDQLRLPRPLATNSFSLLAVFRTTRPHETD
                     PEGKTGVGGVAGQRYLFGAQHGGDANAGAGLSVGTNGVAVYEHGSGYMPALAVYAGPL
                     GSGNVLVGVHYDAKQLSLDVQGLAARTGMVSPRVLVTAPVEIGLGAYGAFDGDLAEVL
                     LYDRPLAENERRGLARFLADKYGIVVAQPRHTNFQLSSKGEDLQLTRPDATIADSLRF
                     GPIPRDISYGRQPDGSGGFVFFREPTPGSPNTTPGATELLDAPVFSHPGGFHANGFDL
                     RLSITNAD"
     gene            1756..2016
                     /locus_tag="PKK20_09900"
     CDS             1756..2016
                     /locus_tag="PKK20_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00241.1"
                     /translation="MRTLIDRTFGRHFHAIYGDSELVIALNPMRVLQGDAPAWVRAWA
                     LDWVKTHEGDFRPVSSDRTGPHVSWNLERREAASCLRWASTM"
     gene            2099..3910
                     /locus_tag="PKK20_09905"
     CDS             2099..3910
                     /locus_tag="PKK20_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNV00242.1"
                     /translation="MSTVPVNPDAVPTPSDFIRDIVAADAQAGKYSQIVTRFPPEPNG
                     YLHIGHAKSICLNFGIAREFGGVCNLRMDDTNPTKEEVEYVESILEDVKWLIAGWADD
                     RIGLKRKGQTPDRVVRDGHPDFHLGAVPRNLLSATEVEPFYASDYFAQLYEYALELIR
                     RGRAYVCDLSPGDTDAYRGAPDRAGQDSPFRNRTVAENLDLFQRMKAGEFPDGTRTLR
                     ARIDMASPNVWMRDPVLYRIRHAEHHHTGDAWCIYPMYDFAHCLSDYIEGITHSICTL
                     EFEVHRPLYDWILEALDLPRPLPHQFEFARLNLSYTVMSKRKLLQLVSEGRVKGWDDP
                     RMPTISGLRRRGVTATALRRFAYNIGVTKYNGVTDVAVLEHAIREELNREAERRLGVL
                     RPLKVVLTNYPEGKVETVDAVNNPENPSAGRRPVPFGRELYIEREDFMEAPPPKFFRL
                     RPGGEVRLKYAYIVRCDEAVKDASGNIVELRCTADLESKSGGAHADRKVKGTIHWVSA
                     AEAVEAEVRLYDRLFTVAQPDSGGEGVDFKTFLNPRSLETVRARVEPSLAEADMGGRY
                     QFERLGYFCLDPDSRRGQLVFNRIIPLKDSWAKEARK"
     gene            3919..>5195
                     /locus_tag="PKK20_09910"
     CDS             3919..>5195
                     /locus_tag="PKK20_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,6-beta-D-fructofuranosidase"
                     /protein_id="HNV00243.1"
                     /translation="MNRSRCVCCSSLSRLLSSRVVLATLLAASVCGGSAAEPSARPDI
                     LIADFERADYGDWTTTGEAFGPGPARGALPDQMAVEGYLGERLVNSFFNGDGSTGTLT
                     SPPFRIERRYLQFLMGGGMHPGQACMNLVLDGQVVRSATGPNDRPGGSERLDWQQWDV
                     GEFGGLEARLQIVDSATGGWGHINVDHIRLSDRPLPGLLTNVARAFTVEGRYLNLPVK
                     HRAPKRRVRLEIESRVVREFEIELADAAPDFWVFLDLGPFEGRRGALVVDRLPEDSKA
                     LDAIDQTDAFRGADGLYREPLRPQFHFSSRRGWNNDPNGLVYYRGEYHLFYQHNPYGW
                     DWGNMHWGHAVSTDLVHWRELPIALYPMAYGDWAFSGSAVVDARNTGGFKTGSEEVLV
                     AAYTSTGRGECIVYSNDRGHSWTEYAGNPVVKH"
BASE COUNT          909 a         1545 c         1789 g          952 t
ORIGIN      
        1 cgtccgcatt ggtgatgctc agccgcagat cgaacccgtt ggcatgaaaa ccgcccggat
       61 gcgagaacac cggcgcgtcc agcagttctg tggcgcccgg cgtggtgttg ggtgagccgg
      121 gggtcggttc tcggaagaag acaaagccgc ccgaaccgtc cggctgacgt ccgtaagaaa
      181 tgtcgcgggg gatggggcca aagcggagag aatcggcgat cgtcgcgtcg ggtcgggtga
      241 gttggaggtc ctcgcccttg ctgctgagtt ggaaattggt gtgacgcggt tgggcgacaa
      301 cgatgccgta cttgtcggca aggaatcggg cgaggccgcg ccgttcgttt tcagcgagcg
      361 ggcggtcata gagcaggacc tcggccaggt cgccgtcgaa cgcgccgtac gcccccagtc
      421 cgatctcgac cggcgccgtc acgaggactc gaggcgagac catgccggtg cgggcggcaa
      481 ggccctggac atcgaggctg agttgcttgg cgtcgtagtg gacgcccacc aggacgttgc
      541 ctgatccgag cggcccggca tagacagcca gggcgggcat gtagcctgaa ccgtgctcgt
      601 acacggcgac gccgtttgtc cccacggaga gcccggcgcc cgcgttcgcg tcgccgccgt
      661 gttgcgcgcc gaacaggtag cgctggcccg ccacaccccc cacgccggtc ttgccctcgg
      721 ggtcggtctc gtgggggcgg gtggtgcgaa acacggcaag gagcgagaag ctgttggtgg
      781 cgagggggcg ggggagccgc aactggtcat ccacgccgtc aaagcggagc gcgggcgtgc
      841 ctgtgggacc ggcggcgagc cacgtcggtt ggttggcgcc cgtggtttgg acggcgtggt
      901 ttcctgcgcc gctctgatcg cgccactgtc gcacgtagac ggtggtgccg tcgcggcgga
      961 cctgagtcga ggaattggga ttcacggcgt cggcacgcag ccacaggcgc aggccggaga
     1021 tggtatcggg ccccatagag tcgacgctgg cgggctgtcg atccttgccc gacgcaaaga
     1081 gcagcaggtg ctctccggcc cctagcacgg cattgcgaaa cacccacttg aaagggcgcc
     1141 gcacgtcgtc cgacacgccc cagccatcca ggctgaccgc gtcggacgtg gggttgaaca
     1201 gttcgaacca gtcgggaaag tcgccgttct cgtcggcggg atttgcgcca ttcgagctca
     1261 tgagttcgtt gatgcgaatc tcctgggcgc gcccgacagc cggatcgaga tcgagcagcg
     1321 ccgacagcca ggcagcggct gcgatggcga tcagcgacac agaacggcaa gtcatgcaaa
     1381 acacgccagc atccttgaaa cccgaaatct cgggcagtca agtcaagttt tggaaagccg
     1441 gaatcctgag gtgcatctga ttccggatgt actgggaatc caggcccggg tcggcgggga
     1501 tgccggactt tgcacaccgg tatgcacgat ttgcacattt ggagagcggg tttcgggcca
     1561 gtcctgccgg ttttgggcgg attcgagcaa ttcttgggct ggcatccttt ctgcgtgtct
     1621 gggtctgcgc ggactgccag agggctgaaa ttgaaacaaa ccggagcatt tcagtgtcct
     1681 tgatgagcag cgtgcagaga actaagaacc gttggaaccg atatgccagt gcttgccaaa
     1741 tctcgcggga tcgtcatgcg gacactaatc gatcgaactt tcggaaggca tttccacgcc
     1801 atctacggcg attccgaatt ggtcatcgcc ctcaacccga tgcgggtgct gcaaggggac
     1861 gcgccggcgt gggtgcgggc ctgggcgctg gattgggtga agacgcatga gggtgacttc
     1921 cgccccgttt cgagcgaccg gactggtccg cacgtttcat ggaatcttga gcgccgcgag
     1981 gctgcaagtt gcctgaggtg ggccagcacg atgtagcatc gtggaattgc ctacccttcg
     2041 gactcgtgct ttgccacact tgcgtcgcac gtgaatccat gatggacgac gcaaagccat
     2101 gagcacagtt ccggtgaatc cagacgcggt tccgactcct tcggacttca ttcgcgacat
     2161 cgttgcagcc gacgcgcagg cggggaagta cagtcagatt gtgacgcggt ttccgcccga
     2221 gcccaacggg tatctgcaca ttggacacgc gaagtcgatc tgcctgaact tcggcatcgc
     2281 gcgcgagttt gggggggtgt gcaacctgcg gatggacgac accaatccga ccaaggaaga
     2341 ggtcgagtac gtcgagtcga ttctcgagga cgtgaagtgg ctgatcgcgg ggtgggcgga
     2401 cgatcggatc ggtttgaagc gcaagggcca gacgcccgac cgggtggtcc gcgacggtca
     2461 tccggatttc catcttgggg cggttccgcg gaatcttctg tcggcgacgg aggttgagcc
     2521 gttctatgcg tcggattact ttgcccagct ttacgagtac gcgctcgaac tgatccgccg
     2581 gggtcgggcc tatgtatgcg acctgtctcc gggcgacacc gacgcctacc gcggggcgcc
     2641 ggatcgcgcg gggcaggaca gcccatttcg gaaccggacg gtggcggaga atctggattt
     2701 gttccagcgc atgaaggcgg gcgaattccc ggacggcacg cggacgctgc gggcgcggat
     2761 cgacatggcg tcgccgaacg tctggatgcg cgatccggtg ctctaccgca ttcggcatgc
     2821 ggagcatcac catacggggg atgcctggtg catatacccg atgtacgact tcgcccattg
     2881 cctgagcgat tacatcgagg ggatcacaca ctcgatttgc accctcgagt tcgaggtcca
     2941 ccgccccctc tacgactgga ttctcgaagc gctcgatctg ccgcgtccgc tgccccacca
     3001 gttcgagttc gcgcgcctga acctctctta tacggtgatg agcaagcgca agctgctgca
     3061 actggtcagc gaggggcggg tgaagggatg ggacgatccg cggatgccaa ccatttccgg
     3121 cctgagacgg cgcggcgtca cggccaccgc gttgcggcgg ttcgcgtaca acatcggcgt
     3181 gaccaagtac aacggggtga ccgatgtcgc cgtgctcgag cacgcgatcc gggaggagtt
     3241 gaaccgcgag gccgaacgcc ggctcggcgt gctgcgtcca ctcaaggtcg tgctcacaaa
     3301 ctaccccgag gggaaggtcg agacggtcga tgccgtcaac aaccccgaga atccgtccgc
     3361 cggacgccgc ccggtcccgt ttggccgcga actctacatc gagcgtgagg atttcatgga
     3421 ggcgccgccc ccgaagttct ttcggctgcg acccggaggc gaggtccggc tcaagtatgc
     3481 gtatattgtc cggtgcgacg aggcggtcaa ggacgcgtcc ggcaacattg tcgaactgcg
     3541 ctgcacggcc gatctcgagt cgaagagcgg gggcgcgcat gcagaccgga aggtgaaggg
     3601 gaccattcac tgggtcagcg cggctgaggc cgtcgaggcc gaggtgcgcc tgtacgatcg
     3661 tctgttcacg gttgcgcagc cggacagcgg gggcgaggga gtcgatttca agacgtttct
     3721 caatccgcgc tcgctcgaga cggtgcgcgc cagggtcgaa ccgtcgcttg ccgaggcgga
     3781 catggggggg cgttaccagt tcgagcggct tggctatttc tgcctcgatc ccgattcccg
     3841 gcgcgggcaa cttgtcttca accggatcat ccccttgaag gacagttggg ccaaggaagc
     3901 ccgcaaatag aggctggcat gaaccgatca cgttgtgttt gctgctcctc tctgagccgc
     3961 ctgttgtcgt ctcgagtcgt cctggccacc ctccttgcgg cttctgtgtg cggtgggtcc
     4021 gcggcggaac cctcagcgcg ccccgacatc ctgatcgcgg acttcgagcg tgccgattac
     4081 ggcgactgga cgacgaccgg agaggccttt gggccgggac ctgcgcgtgg tgctttgccc
     4141 gatcagatgg ccgtggaggg ttacctcggg gagcgcctgg tcaactcgtt cttcaatggt
     4201 gacggctcca ccgggacact cacgtcgccg cccttccgaa tcgagcggcg ctatctgcag
     4261 tttctgatgg gcggcggcat gcatcccggc caggcgtgca tgaacctggt cctggacggg
     4321 caggttgtgc gcagcgctac gggtccgaac gatcggccgg gcggcagcga gcgtctcgac
     4381 tggcagcagt gggatgtggg cgagttcggt gggctcgagg ccaggctgca gattgtggac
     4441 agtgccaccg gcggttgggg ccatatcaac gtcgatcaca tccgtctgag cgaccgcccg
     4501 cttccgggtc tcctgacgaa cgtggcgcgc gcgttcacgg tcgaagggcg gtacctgaat
     4561 ctgccggtca aacaccgggc tcccaaacgc cgggttcgat tggagatcga gagccgcgtg
     4621 gttcgggaat tcgagatcga gctggccgac gcggcgcccg atttctgggt gtttctggat
     4681 ttggggccgt tcgaggggcg gcgcggggcg cttgtggtgg atcggctgcc ggaggattcg
     4741 aaggcgctcg acgcgatcga tcagaccgac gcctttcgcg gcgcggacgg gctctatcgc
     4801 gagccgctcc ggccccagtt ccacttctcc tcacggcgcg gatggaacaa cgatccgaac
     4861 ggcttggtgt attaccgcgg cgagtaccat ctgttctacc agcacaatcc gtacggttgg
     4921 gattggggca acatgcactg gggccacgcc gtcagcaccg acctggtgca ttggcgcgag
     4981 ttgccgatcg ctctctaccc catggcctat ggggactggg ccttttcggg cagcgcagtc
     5041 gtggacgccc ggaacacggg cggattcaag actggcagcg aggaggtctt ggtcgccgcc
     5101 tacacgagca cgggccgcgg cgagtgcatc gtctactcga acgatcgcgg acactcctgg
     5161 accgagtatg ccgggaaccc ggtcgtcaaa cacga
//
LOCUS       DAOQRO010000263         5147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16837_length_5147_cov_4.049097, whole genome shotgun sequence.
ACCESSION   DAOQRO010000263 DAOQRO010000000
VERSION     DAOQRO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16837_length_5147_cov_4.049097"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..572
                     /locus_tag="PKK20_09915"
     CDS             159..572
                     /locus_tag="PKK20_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV00244.1"
                     /translation="MKGAVLLDTGPLVAFLAAGLEHHEWVCEQWKRLHPPLLTCEPVL
                     TEAAFLLKREGRESDPLFALLERGVLRAGLEVEDQLADLRALMRRYRDRSMSLADACL
                     VRSAELHPGGIIFTLDEDFRVYRRHGNKVIPMLMP"
     gene            complement(686..2986)
                     /locus_tag="PKK20_09920"
     CDS             complement(686..2986)
                     /locus_tag="PKK20_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00245.1"
                     /translation="MKALIFACWLAALPLDAADAAVPVTLTAGTDWIPLEPDLDIEPG
                     SALDFSSMGFVDPPAGQHGRVIVSPDGHFAFERSARTPRRFYGVNLCFGAHFVSKPEA
                     DRLADRLVRLGYNALRIHHYERELTQGQRPTTTLNPQKLDQFDYLMAALIKRGIYLTT
                     GLFVSRGVPWREIGVDRNGEVPMDTFKILVPVHAGAYENWKQFSRSLLDHVNPYTQRR
                     YADEPALAWLSMINEGNFGNFLKDLRAFDEWRQAWNRWLAKRYADRAALAAAWGDELG
                     SDEDPAKASVALPDNIYDSKVRTRDCIAFFGDTERDMVLRMTRFLREDLGCRALVSNA
                     NSWTRFTTDQAARAVYDYVDDHFYVDHPQFLEGAWRLPSRCPNTSPIATGATGGRNLS
                     FTRLFDRPFTITEYNYSAPGRFRGVGGILTGALGALQDWDGIWRFAYSHGREAMFTPS
                     RMNYFDMACDPLGQAAERASLCLFLRGDLQPAPHSVAVVMTPDDLANPPRKIPTLAPR
                     WHWLAWITRIGTRVMPSPTLPLPHDILAPLGWKTSKDEFAAAAAAVSVPSAERTKPVL
                     LADPYDLDDAALWSAVQRLPLAKPGNLTDPGRKIYQSETGELTIDAPGDLLVLDTPRT
                     AGGYATVGHSIEAPGGGVSVRMDGSNATVWVSALDENPIRRSRRLLVTHLTDLQNTGI
                     EYAEETRQTLRNWGRMPHLVRAGKAHLAIALANPDRYEVWALAPGGRRLAQVPVTVEG
                     GALRFTADVAGDARQGARMLYEVAMK"
     gene            3119..4405
                     /locus_tag="PKK20_09925"
     CDS             3119..4405
                     /locus_tag="PKK20_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNV00246.1"
                     /translation="MKFETLCLHGGTQPDPTTLARAIPVYRTASYNFRSTEHAANLFA
                     LKELGNIYSRLTNPTTEALEKRVALLEGAPELGALATASGTSGVFYSIINLARAGDNV
                     VSARNLYGGTYTQFNDILPALGIEIRFVDSNDPENFGKVIDGRTRALFCEAVSNPALE
                     VTDLEGVAAVARREGVPLIVDATFSTPYLTRPLAHGADIVVHSLTKWFGGHGTGIGGI
                     VVDGGRFPWAAGKHPLYTEPDASYHGLRWGVDLPEPLRALAFILRMRTVALRNLGACL
                     SPDSSWLFLQGIETLPLRMERHCDNARAVARYLKSHPRVEWVRFPGLESDPMFELNTK
                     YLRGKGGSMVVFGIRGGKTSGAKFIDALKLISHLANVGDAKSLAIHPATTTHSQLTEA
                     QQRAGGIAPELVRLSIGLEHIDDLLADIEQALSRTA"
     gene            4569..>5147
                     /locus_tag="PKK20_09930"
     CDS             4569..>5147
                     /locus_tag="PKK20_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV00247.1"
                     /translation="MTRSIGKVRTQFFEFHNRKRPFQLRLGPVLPRFTLAYEMYGRLN
                     ANRSNAILLFHAMTGSQHAAGLNREVPGLDGRWTDEMHEGWWDDFIGPGKALDTRRFC
                     VICANYLGGCYGSTGPASVNSDTGRRWGPGFPVLRMRDIVDSQMALLDHLRVRRLHAV
                     IGASIGGFLCQVLATRHPDRVRIVVPIGTGIET"
BASE COUNT          886 a         1506 c         1792 g          963 t
ORIGIN      
        1 cctctcccac gagacctcaa gtgaacgaag gcgctggacg cgcgcgcgtc cagcgacaag
       61 gccacctgct acgatctggc gcgcgacctg gccgggtcgg tgaaatggct gccgcgcgat
      121 ctggccacga atccaaagta tctgaatggc tttgggcgat gaaaggcgcg gtgctgctcg
      181 acaccgggcc attggtggcg tttctcgccg ccggcctgga gcatcatgag tgggtctgcg
      241 agcagtggaa aagattgcac ccgccgctgt tgacttgcga gccggtgctc acggaagccg
      301 cttttctcct gaagcgggaa ggccgggaga gcgacccgct ctttgccctg ctggagcgtg
      361 gcgtgttgcg ggcggggctg gaggtggagg atcaactggc ggacctgcgc gcattgatgc
      421 gccgctaccg ggatcgctcc atgtccctgg ccgatgcctg tctggtccgg tcggcggaac
      481 tccatccggg tggcatcatc ttcaccttgg acgaggattt ccgagtctat cgccgtcatg
      541 ggaacaaggt catcccgatg ttgatgccgt aaaggcaatg ggttctggcc tgggtacagg
      601 cgcgcgtctg gtctcaactc ttgcgggcat tgaagggcca tgccggacca gtcggaacga
      661 attccatcgc accagggaaa gatggtcatt tcatcgccac ctcgtagagc atgcgggcgc
      721 cttggcgggc gtcgccggcg acatccgcgg tgaaacggag cgcgcctcct tcaaccgtca
      781 cggggacttg agccaatcga cgcccgcccg gcgccagggc ccatacttcg tagcggtccg
      841 gattggccag cgcgatggcc agatgcgcct tgccggcgcg gacgaggtgc ggcattcggc
      901 cccaattgcg cagggtctgg cgcgtttcct ccgcgtactc gatgccggtg ttttgcagat
      961 cggtgagatg ggtcacaagc aggcgccgac tacgacggat cggattctca tccagggcgc
     1021 tgacccagac cgttgcgtta ctgccgtcca ttcgcaccga gacgccgcct ccaggggcct
     1081 cgatcgaatg gccgacagtt gcgtacccgc cggcggtgcg cggggtgtcc aggacgagca
     1141 ggtcgcccgg ggcatcgatg gtcaattcgc ccgtctcgct ctggtagatt ttgcgtcccg
     1201 ggtcggtgag attgcccggt ttggcgagag gcagacgctg cacagcggac cagagggctg
     1261 cgtcgtcgag atcgtacgga tcggcaagca gcacgggctt cgtccgctcg gcgctgggga
     1321 cgctgacggc ggcagcggcg gcggcgaatt catccttcga cgtcttccag cccagggggg
     1381 cgaggatgtc gtgcggcagc gggagagtgg gggaaggcat gacccgcgtg ccgatgcggg
     1441 tgatccaggc cagccaatgc catcgagggg cgagggtggg gatcttgcgg ggcggattgg
     1501 caaggtcgtc cggggtcatc acgacggcga ccgaatgggg ggccggttgg aggtcgccgc
     1561 ggaggaagag gcagagcgac gcgcgctcgg cggcctggcc gagcgggtcg catgccatgt
     1621 cgaagtagtt cattcgggag ggagtgaaca tggcctcgcg cccgtggctg taagcgaaac
     1681 gccagatacc gtcccagtcc tgcagcgcgc ccagcgcccc cgtgaggatg ccgccgacac
     1741 cgcggaaccg gcccggggcg gagtagttgt actcggtgat ggtgaacggg cggtcgaaga
     1801 gtcgggtgaa gctcaggttg cgtccgccgg tggcgccggt ggcgatcggg ctcgtgttag
     1861 gacagcggct ggggagccgc cacgcgcctt ccaggaactg cggatggtcg acgtagaaat
     1921 ggtcgtcgac gtagtcgtag accgcgcgcg ccgcctggtc ggtggtgaat cgcgtccagg
     1981 agttggcgtt gctgacgagc gcgcggcagc cgaggtcctc cctcagaaat cgcgtcatcc
     2041 gcagcaccat gtcgcgctcc gtgtcgccaa agaacgcgat gcaatcccga gttcgcacct
     2101 tgctgtcgta gatgttgtcg ggcagtgcca ccgacgcctt tgcaggatcc tcatcggatc
     2161 ccagctcgtc gccccaggca gccgccaggg cggcgcggtc ggcgtaacgc ttcgccagcc
     2221 agcggttcca ggcctggcgc cactcgtcaa aggcccgcag gtccttgagg aaattgccga
     2281 aattgccctc attgatcatc gaaagccagg ccagggccgg ctcgtccgcg taccggcgct
     2341 gcgtgtaggg attgacatga tcgagcagcg atcggctgaa ctgcttccag ttttcgtacg
     2401 cgcccgcgtg gaccggcacc agaatcttga acgtgtccat cggcacctcg ccgtttcggt
     2461 cgaccccgat ctcgcgccac ggcacgcccc gggagacgaa caggccggtg gtcaggtaaa
     2521 tgccgcgttt gatgagcgcg gccatgaggt agtcgaattg atcgagcttc tgcggattga
     2581 gcgtggttgt tggacgctgg ccctgggtca gttcgcgctc gtaatggtgg atgcgcaggg
     2641 cattgtagcc cagccggaca aggcgatccg ccagtcgatc ggcctccggt ttcgacacga
     2701 agtgcgcgcc gaaacagagg ttcacgccgt agaaccgccg gggcgtccgc gcagaacgtt
     2761 cgaaggcaaa atgaccgtcc gggctcacaa tcacccgccc atgctggccc gcgggaggat
     2821 cgacaaagcc catcgaagag aagtccagag ccgagccggg ttcgatgtcc aaatcgggct
     2881 cgagcggaat ccagtccgtg ccggctgtga gtgtgaccgg gaccgcggca tccgcggcgt
     2941 cgaggggcag ggcggctaac cagcaggcga agatcagggc tttcatggcg taatacgttc
     3001 cagtgttttg tgatgcatca tcggacgccc cggggcggga ggcaagcgcg ctgttcgagc
     3061 gcgcctggcg tgtttcggat tggacagacg ggaaagtgaa ctgtaagatt tctacttaat
     3121 gaaattcgaa actctttgtt tgcatggcgg cacgcaaccg gatccgacga ctctggcgcg
     3181 ggcgattccg gtgtatcgga cggcctcgta caacttccga agcacggagc acgcggcgaa
     3241 tctctttgcg ttgaaggagc tggggaacat ctacagtcgg ctgaccaatc cgacgacgga
     3301 ggctttagag aagcgcgtgg cgttgctcga gggcgctccc gagttgggcg ccctggcaac
     3361 ggcctcgggg acgagcggcg ttttctactc gattatcaat ctcgccaggg ccggcgacaa
     3421 cgtggtcagc gcccgcaact tgtacggagg cacctacacc cagtttaatg acatcctgcc
     3481 cgcgctgggc atcgagatcc ggttcgtcga ctcgaatgac ccggagaact tcggcaaggt
     3541 gatcgatggc cggacacgcg cgctgttttg cgaggcggtg tccaatccgg cgctcgaagt
     3601 caccgatctc gagggcgtgg cggcggtggc ccggcgggag ggtgttccgt tgatcgtgga
     3661 tgcgactttt tcgacgcctt atctgacgcg acctctggcg catggggcgg acattgtggt
     3721 gcattcgctg acgaagtggt ttggggggca tgggactggg attgggggga tcgtggtgga
     3781 tggcgggcgg ttcccctggg ccgccggcaa gcatccgctc tacaccgagc cggatgccag
     3841 ctatcacggc ctgcggtggg gggtggattt gcccgaaccg ctgcgcgcgt tggcgttcat
     3901 actgagaatg cggacggtgg ccctgcgcaa tctgggggcc tgcctttcgc cggacagttc
     3961 gtggctattc ctgcaaggca tcgagaccct tccgctgcgg atggaacgtc actgcgacaa
     4021 tgccagggcg gtcgcccggt acttgaaatc gcatccgcgg gtcgaatggg tgcgctttcc
     4081 cggtctggaa agcgacccga tgttcgagct caacacgaag tatctgcggg gcaagggcgg
     4141 gtcgatggtc gtgttcggca tccggggtgg aaagacgtcc ggagcgaagt tcattgatgc
     4201 cctgaagttg atatcgcacc tggccaacgt gggcgatgcg aagagcctgg caattcaccc
     4261 ggccacgacc acgcattccc agttgaccga ggcgcagcag cgggccgggg ggatcgcgcc
     4321 cgagttggtc cgcttgagca tcggactgga gcacatcgac gatctgctcg ccgatatcga
     4381 acaggctctg agccgcacgg cgtaagcatc cgttcgggcc tctgcaacaa gcaacgtccc
     4441 attctcaccc gaactgccat tcctcgaacg aatgggtgat tcgcggagag cgttggcact
     4501 cagcggttga atgcggacgg ggcttgcgtc agggggcctg acgtcgcaca ctgcgcgccg
     4561 tttgtttcat gacccgttcc attggcaaag tacgaacgca gttcttcgag tttcataatc
     4621 gcaagaggcc ttttcagttg cggctgggtc cggtcttgcc ccggttcacg ctggcgtacg
     4681 agatgtatgg ccggctaaac gcgaatcgct cgaacgccat cctccttttc cacgccatga
     4741 ccggcagcca gcatgcagcc gggctgaacc gggaggtgcc cgggcttgat ggccggtgga
     4801 ccgacgagat gcatgaaggc tggtgggatg atttcatcgg gcccggcaag gctctggaca
     4861 cccgccggtt ctgcgttatt tgcgcgaact atttgggggg gtgttacggt tcgactggcc
     4921 cggcttcggt gaattcggac acgggccggc gctgggggcc gggatttccg gtgttgcgga
     4981 tgagggacat tgtggattcg cagatggcgc tgctggacca tctgcgcgtg cggcgactcc
     5041 acgccgtcat cggggcgtcg attggcggtt ttctctgcca ggtgctcgcc acacgccacc
     5101 ctgatcgggt gcggattgtc gtgccgatcg gcacagggat cgagacc
//
LOCUS       DAOQRO010000264         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16864_length_5141_cov_4.512584, whole genome shotgun sequence.
ACCESSION   DAOQRO010000264 DAOQRO010000000
VERSION     DAOQRO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16864_length_5141_cov_4.512584"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKK20_09935"
     CDS             <1..300
                     /locus_tag="PKK20_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNV00248.1"
                     /translation="ADAAASAKSPYEQMHVEFLQSIRDGTPFQRAKTLADSTMATVVG
                     QMAVYSGQKTTWKDAWDSGFAFQPAGEVTMDTEPPVKPGADGIYPVAIPGQTKLI"
     gene            332..2476
                     /gene="ppk1"
                     /locus_tag="PKK20_09940"
     CDS             332..2476
                     /gene="ppk1"
                     /locus_tag="PKK20_09940"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNV00249.1"
                     /translation="MAEGPNRYINRELSWLEFNQRVLDEALDCRNPLLERVKFFCIVS
                     SNLDEFFEVRVAGLKQQVQSGVAERSPDGLTPTECCRAIARRVRRMVKDQYTCWREQL
                     VPELGRHGIRFTRVADLDEEDRQWLDRFYRAEVRPVLTPLSIDPAHPFPQLLNKRLNL
                     IVQVRANIHGRKRGRLAVVEVPRILPRLIELPRDGGAKEYVFLGNLIGHYLADLFPGM
                     LILGHWLFRVTRNSELYLDEEDVANLLSAVETRLHRRLKGEAVRLEVERDCPIDIRDE
                     LLRTLHLDEDDLYVLDGPLNPTRLMTLCPNDRQPELHDPPFLAPAPLVLRDAPDLFAA
                     IRDRDILLHHPYESFECVVGFLQQAATDPRVLAIKQTLYRTGGDARIIGALMDAVHNG
                     KQVTAVVELKARFDEANNIEWSRQLEQAGVYVVYGLVGLKIHCKVTLVVRRDDDGIRR
                     YLHLSTGNYNPTTAKLYTDIGLLTCHPDFGEDATCLFNLLTGICQFQETRKFVVAPFE
                     LHDRMLALIRREADNARRGLPARIVAKMNSLVDRKIIDALYEASQAGVRIDLVVRGIC
                     CLRAGQPGLSETITVRSIVGRFLEHSRIFAFHNACRPEVWLSSADWMPRNFFKRIEVA
                     FPIEDGNLRDRVLWELDSVILGDTVKARALFPDGSYQRVRVPRGAKPLDSQAELIRRA
                     LEGAASTPGRSESASSYPKVRLAPRPRAKAGS"
     gene            complement(3189..4706)
                     /locus_tag="PKK20_09945"
     CDS             complement(3189..4706)
                     /locus_tag="PKK20_09945"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00250.1"
                     /translation="MNAPSMNRRHFLASSGAAATAFTIIPAAVLGRTGETAPNSRINV
                     AFIGVGSQGLRVMISFLKQPEVQAVAVCDPVKSAADYHQWGQSEFANEVHRLLGVSSG
                     WEWLSPNQPMLELTPTMKVPAGVCGREPAQKIVDAWNAKRSGATRSRGCSSYADFREL
                     LDRERDLDAVVVCSPDHLHAAISIAALKAGKHVYCQKPMTRTIHEAGRMAAVAKESGL
                     ATQVAVGPQASEDTRRLCEWVGAGAIGKVREVINWSSRPFWPQGIPAPAAGQPVPEGM
                     DWDLWLGPASERPFHHAYLPFVWRGWVDFGCGAFGDMGCYSFDTIFRVLGLDAPVGAE
                     ASSTNRYAASYPLASMVHLEFEARGGQAPVKLRWFDGGLRPARPSILDASEKLEAEGL
                     MFVGDQGTILADFVGGRPRLIPSAAMRAFKEPPKTLPRSPGNEREWLDAIGDRRKSTG
                     ANFVFSSKVTEALCLANMAIRCGEPLGWDSAALKVAGPAEAQQMVNPPAREGWGV"
BASE COUNT          914 a         1648 c         1645 g          934 t
ORIGIN      
        1 gctgacgccg cggcttcggc aaaatcgccc tacgagcaga tgcatgtcga gttcctgcaa
       61 tcgatccggg acgggacgcc gttccagcgg gcgaagaccc tggccgattc gactatggcg
      121 accgtggtcg ggcagatggc agtctactcc gggcagaaga ccacatggaa ggacgcttgg
      181 gattcggggt tcgcttttca acccgcgggc gaggtgacga tggatacgga gccgccggtc
      241 aaacccggcg ccgacggcat ctatccagtg gccattcccg ggcagaccaa gctgatttga
      301 cgcgggtcgc ggtcagcatg gcgaggatgc tatggccgaa ggtcccaacc gctacatcaa
      361 ccgggagctt agttggctcg agttcaatca gcgtgtgctc gacgaggcgc tggactgtcg
      421 caatccgctg ctcgagcggg tcaagttctt ctgcatcgtc agttcgaacc tcgacgagtt
      481 cttcgaagtc cgggttgccg ggctcaagca gcaggtgcag agcggtgtcg ccgagcgatc
      541 tcccgacggg ctgacgccga ccgagtgctg ccgggcgatc gcgcggcgcg tccgacgcat
      601 ggtcaaggat caatacacct gttggcggga gcaactggtg ccggagcttg ggcggcatgg
      661 gattcgattc acgcgggtcg ccgacctcga tgaggaagat cggcagtggc tggaccggtt
      721 ctatcgcgcg gaggtccgtc cggtgttgac accgctttcg atcgacccgg cccatccgtt
      781 tccgcagctt ctcaacaaga ggctcaatct catcgttcag gtgcgggcga atatccatgg
      841 tcgcaagcgc gggcggctgg ctgtggtgga ggtgccccgc atcctgccgc gtctgatcga
      901 gcttccccgc gacggcggcg ccaaggagta cgtgtttctc gggaacctga tcggccacta
      961 cctggctgac ctgttcccgg ggatgctgat cctggggcac tggctgttcc gagtgacgcg
     1021 gaacagcgag ctctacctcg atgaggagga tgtggccaac ctgctcagcg cggtggagac
     1081 gcgactgcat cgccgactca agggcgaggc ggtgcgtctg gaggtggagc gcgattgccc
     1141 gatcgatatc cgggacgaac tgctgcgaac cctgcatttg gacgaggacg acctttacgt
     1201 cctggacggg ccgctgaacc cgacgcgcct catgacgctc tgcccaaacg atcgccagcc
     1261 cgagctgcat gatccgccct tcctggcgcc ggctcccctg gtcctgcgcg atgcgcccga
     1321 cctctttgcg gcgatcagag atcgggacat tctgctgcat catccgtatg agagcttcga
     1381 gtgcgtggtc ggcttcctgc agcaggcggc caccgacccc cgcgtgctgg ccatcaagca
     1441 gactctctac cgcaccggcg gggacgcgcg gatcataggc gcgctcatgg acgcggtgca
     1501 caacggcaaa caggtcaccg ccgtagtcga gttgaaggcg cgtttcgacg aggccaacaa
     1561 catcgaatgg tcgagacagc tcgaacaggc cggagtttat gtcgtctacg gtttggtcgg
     1621 cctcaagatc cattgcaagg tgacgctggt ggttcggcgc gacgacgacg gcatccggcg
     1681 ctatctgcac ttgagcacgg gaaactacaa tcccaccacg gcgaagctct acacggacat
     1741 cgggctgctc acgtgccacc ccgatttcgg cgaggatgcc acctgcctgt tcaacttgct
     1801 gacggggatt tgccagttcc aggagactcg gaagtttgtg gtggcgccgt tcgagcttca
     1861 tgaccgcatg ctggcgttga tccggcgcga ggccgacaat gcgcgccgcg ggctgccggc
     1921 tcgcattgtc gcgaagatga actcgttggt cgaccggaag atcatcgatg ccctctacga
     1981 agcctcgcaa gcgggtgtgc ggatcgatct tgtcgtgcgc ggaatctgct gcctgcgggc
     2041 cgggcagccc ggtctgagcg aaacgatcac cgtgcggagc atcgtcgggc gtttcctgga
     2101 acatagccga atctttgcct tccataacgc ctgccggccc gaggtctggc tttcgagcgc
     2161 cgattggatg ccccggaatt tcttcaagcg catcgaggtg gcgtttccca tcgaggacgg
     2221 gaacttgcgc gaccgcgtcc tgtgggaact ggactcggtg atcctgggcg acactgtcaa
     2281 ggcccgcgcg ctgttccccg atgggagtta ccaacgcgtc cgcgtgccgc ggggagccaa
     2341 gccgctggac agccaggctg aactgatccg ccgggcgctc gagggagccg cgtcgactcc
     2401 aggacgcagc gagtccgcgt cttcgtaccc gaaggtgcgc ctggcgccgc gccccagggc
     2461 gaaggcgggg agctgagggc aagacggggc gcatctcagc tttttcctgg gggggtacca
     2521 tttgtgaacg accggtcagg tgattgaggc gtggggggag cggtggttgg tgagttcacg
     2581 ggttgggttt tcgggttggc tctggtggtc ctcaaacggc tgctccaagc ccgtcttcaa
     2641 agacatggcc gcccgcacct tgttgctcaa gctggagcaa cgcgggtgga ttcgtctgcc
     2701 ccccgccgct gttgcccgtc caaccgcatg cgccgggctc ttgcatcgcc ggaggtcccg
     2761 gcggtgcccc ccactgtgtt gatcgctccg ttgtcggcgc tgctccccct gcgcatcaca
     2821 gaaatcagca ggcagggctc tccgcccgtt caggtcgatt tccgggagat tgtgggcggg
     2881 cgcagcatcc tgaaagccgc atcggacgta tacgcggggc atcctctcgt cgtgatgggg
     2941 gaagcctctg gcgagaggag gcatgccggg gcaaaccgat ggcgagcgtg tggtgcgaac
     3001 gctgatcttg ttggtggcgt ttccggccct cgggcacacc aatcggcacg gcccgttccg
     3061 cttgcatttg ctcaagataa tctcgttcct caaacaacaa ctcggactcg cggttccggc
     3121 cgagcggcgg gaatgcatcg agcggatgct tgtcaccgct cgagctcgag ttcttcccgc
     3181 acaccacact acacgcccca gccttcgcgg gcgggcgggt taaccatctg ctgcgcctcg
     3241 gcagggccgg caaccttcaa ggcggcgcta tcccagccca gaggctcgcc gcagcggatc
     3301 gccatgttcg ccaggcagag cgcttcggtc accttcgatg aaaagacgaa gttcgccccg
     3361 gttgacttgc gccgatcgcc gatcgcatcg agccactcgc gctcgttccc cggcgaacgc
     3421 ggcagtgtct tcggcggctc cttgaaggcc cgcatggccg ccgacggaat caggcgcggc
     3481 cgccccccca cgaaatcagc cagaatggtc ccctgatcgc cgacgaacat cagcccctcg
     3541 gcttcgagtt tctcggaggc atcgagaatc gagggccgtg cggggcgcag accgccgtcg
     3601 aaccatcgca acttcaccgg cgcctgcccg ccgcgcgcct cgaactcgag atgcaccatc
     3661 gaggccaatg gatagctcgc cgcgtaacga ttggtcgaac tcgcctccgc gccaaccggc
     3721 gcatcgagtc cgagcacgcg gaagatggtg tcgaaactgt aacagcccat gtcgccaaac
     3781 gcgccgcagc caaaatccac ccacccgcgc cacacaaacg gcagatacgc gtgatggaac
     3841 ggacgctccg aagccggccc gagccagagg tcccaatcca taccctcagg cacaggctgg
     3901 cctgcggccg gcgccgggat gccctgcggc cagaacggac gcgaagacca gttgatcacc
     3961 tcgcggacct tgccgatcgc ccccgcgccg acccactcgc acaggcggcg ggtgtcttcg
     4021 gatgcctgcg gcccaaccgc aacttgcgtg gccaaaccgc tctctttggc cactgccgcc
     4081 atccgtcccg cctcgtggat ggtccgggtc atcggcttct ggcaatagac gtgcttgccc
     4141 gccttcagtg cggcgatcga tatggccgca tgcaggtggt ccggactgca cacgacaaca
     4201 gcatccaggt cgcgctcccg gtcgagcaac tctcggaaat cggcgtagct cgaacagccg
     4261 cgcgaacgcg ttgcgccgct gcgcttcgcg ttccacgcgt ccacgatctt ctgtgccggc
     4321 tcgcgaccgc agacgcccgc cgggaccttc atggtcggcg tgagttcgag cattggctgg
     4381 ttcggactca gccattccca gcccgagctc acgcccagca aacggtgcac ttcgttggcg
     4441 aactcgctct ggccccactg atgatagtcg gcggcgctct tgaccggatc gcacaccgcc
     4501 accgcctgca cttcgggttg cttgagaaaa ctgatcatca cacgcaaccc ctgcgagccc
     4561 acgccgatga aggccacgtt gatccggctg tttggcgctg tctcccccgt ccggcccagc
     4621 acggccgccg ggatgatcgt gaaagccgtc gcggcggcgc cgctcgaggc gaggaaatgc
     4681 cggcgattca tcgagggtgc attcatagac gatttcattg cgtgttgccg tacatgctaa
     4741 ccgctcggac acgccgcgca agccctgtcg tcccccgaca gcactggcgc ctcgaagacc
     4801 acgcatgaag cccaggggac tcgcccgttc attcgtgccc caccctggtt catgagggaa
     4861 gccgggcgcg gtgactgaca tcgaagggga ttcaccacag agacgcagag ggcacaaagg
     4921 ccatggtcgt catgaatgtt gtgcggatca tgaagggcgg gggactcttg cgttcatgtt
     4981 ccgccctaat tcatgaggag ccctgcgaat gagcaagcgg cggaacaaaa aaaaggggca
     5041 gccaagctgc ccctttcgag atgagattca atcgagcgtg tgcctgctag tgcttacttg
     5101 cactgttaaa cgatatattg tatgttgggg tgcatggcaa g
//
LOCUS       DAOQRO010000265         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16897_length_5134_cov_3.941327, whole genome shotgun sequence.
ACCESSION   DAOQRO010000265 DAOQRO010000000
VERSION     DAOQRO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16897_length_5134_cov_3.941327"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKK20_09950"
     CDS             complement(<1..247)
                     /locus_tag="PKK20_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV00251.1"
                     /translation="MKAQAGTILFACVVGLLGAGCGPSAPSTPLHSAVEDGDYPAVRR
                     HIAARSDLNAKDSSGWTPLHRAAMRGDLPMVQLLADAG"
     gene            542..1183
                     /locus_tag="PKK20_09955"
     CDS             542..1183
                     /locus_tag="PKK20_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNV00252.1"
                     /translation="MQEYKALHDAILSGDAATARAVTEKALAASADPLRLVNDLMVPA
                     MDEVGRRFECNEYFVPELLISARAMKAALELIRPLLIARGDEPAGRVAIGTVKGDLHD
                     IGKNLVAAMLEGGGFEVIDLGVNVSPEQFVKAVREGKANVVAMSALLTTTMPAMKTTI
                     EALEQAGLREKVKVLIGGAPITQKYAEEIGADGYSDNAPAAVSLAKKQLALGA"
     gene            1361..2296
                     /locus_tag="PKK20_09960"
     CDS             1361..2296
                     /locus_tag="PKK20_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNV00253.1"
                     /translation="MKPLLVRLLQDGPVITDGAWGTELQARGLAIGDFPDVWNLSHPD
                     RVTEVARAYVEAGSQIILTNTFGANRIRMADHGAPEQVAEINRRGAQLSIQAAGNRAS
                     VFASIGPTGKLLMAHEVTLDEVRAAFVEQAQALAQGGAHGLVIETMSDLDEAKAALDA
                     AQRTGLPVVVCMVFDAGREKDRTMTGATPEQVARELTGAGADVIGANCGLGIEGYVPV
                     CRRLKAATDLPIWIKANAGLPTLVGGNVVYQTTPDQFASHVPALIEAGASFIGGCCGT
                     NPSFIRAVRDVRGPLPSAERLIARSTRGEPGHPAT"
     gene            complement(2250..2681)
                     /locus_tag="PKK20_09965"
     CDS             complement(2250..2681)
                     /locus_tag="PKK20_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV00254.1"
                     /translation="MLTGLAPPSNIQVHIPSSLPRVRGVAGQLQRIFQNLLDNAIKFM
                     DKPLGVITVRATRLRNAWQFAVADNGPGIEPRYHAQVFGLFERLRVGPHNDGTGIGLA
                     LVKRILEAKGGEITLDSAPGEGSVFTFTWPDAPVRLESNEQ"
     gene            complement(2675..3517)
                     /locus_tag="PKK20_09970"
     CDS             complement(2675..3517)
                     /locus_tag="PKK20_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV00255.1"
                     /translation="MLGSAMQAETVLTVHSDIEEGLVSLRIESGLEQASAARIAKLEA
                     MVRERDELLRLLSVHAPVGFFQTDGAGRLIRTNARWREIARLSHIAAPRGVWWQMVDP
                     GDRERVVGAWKAAVRHGCEFQEEFRTNTGAGWTCYVRTRIVPVIEGGGAVGKCIGVSE
                     DITERRRLESALRRAHEDLERKVKERTAQLQAANMELGQFVHVVAHDLKAPLRAVSHL
                     ADWVASDCASQIDANGHNLLGLLRQRVQYMHDLIEGVLSYTRLGQTKEPEVELTSTPW
                     CVGC"
     gene            3661..>5134
                     /locus_tag="PKK20_09975"
     CDS             3661..>5134
                     /locus_tag="PKK20_09975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV00256.1"
                     /translation="MSADRLFSQPAAGENPIAVAGPIQASPAANSSVDRLNPGGARGR
                     DTLITCRNSQGVEIRATPLRLSRYEAVFEVYNPYSILQLSEVLSDFRITIGERIVYAG
                     RAVVSKLVNASIMVICEVSLESDWLDVEALSPLADPEWLRREFAAFLTDWSKIQAVSP
                     AFKAAVVDMETFLADLRRWLDQIELGIRSHPSHEVEKMEREVMGRLSGTVLDPINELF
                     SRFEELAKSIAPDHHPIHRTYARRQLHPLVLCAPFPHRTFQKPLGYAGDYEMVNMILN
                     DPFEGASLFAKSVNYWFLMQAPAAAHRNRIEYLFQTLVREVQRVAGAGRRARIFNLGC
                     GPAAEVQRFLETQSLSDAADFTLVDFNEDTLSFARHRLAEIKSRTRRSTDFQFIQQSV
                     HQVLKRSARGDKPSAQYDLVYCAGLFDYLSDRICQRLMQYFHEMLAPGGLLISTNVDP
                     SNPNRHGMEYLLEWHLNYRDETVMRTLHPTAARPECITTKA"
BASE COUNT          995 a         1728 c         1519 g          892 t
ORIGIN      
        1 cccccgcatc cgccagcaac tgcaccatcg gcaggtcccc ccgcatcgcc gcccgatgga
       61 gcggcgtcca gccggagctg tccttggcgt tcagatccga gcgggcggca atgtgtcttc
      121 ggacggccgg gtaatcgccg tcctcgacag ccgagtgcag cggggtgctc ggggcgctcg
      181 gcccacaacc ggcgccgagc aggcccacaa cacaggcgaa cagaatcgta ccagcttgtg
      241 ccttcatgcg gacgtgatac cccccggcgg ggcgactggc gagcggaaaa cctccattct
      301 cccgaggacc gggcatcgag ccgcgcgcgc cgctctgcgt ccgggtccgg gctagcccgg
      361 ctcggacgga agctccggaa caacccaaaa cgaaagagtg acaacggcgc cgaaagaatc
      421 catgctttcc gtccaacagc gccgcaggtt tggcagaacc actcgaagcg cgttgctcgc
      481 gatcaacgcg gctgtttgaa cggtccggca gcctggggca agatcatggc aaagacttgt
      541 tatgcaagag tacaaagcgc ttcacgatgc aatcctgtcc ggagacgccg ctacggcacg
      601 ggcagtcacc gaaaaggctc tcgctgcttc ggcggatccg ctccgtctgg taaacgacct
      661 gatggtgccg gcgatggacg aagttggtcg ccgtttcgag tgcaacgagt acttcgtgcc
      721 cgagttgttg atctccgccc gcgcaatgaa agcggcgctt gaactgattc gcccgctgct
      781 catcgcccgc ggcgatgaac ccgccggacg cgtcgccatt ggcacggtca agggcgacct
      841 gcacgacatc ggcaagaacc tcgtcgcagc catgctcgaa ggcggcggat tcgaggtgat
      901 cgacctgggc gtgaacgtct cgcccgagca gttcgtcaag gcggtccgag aaggtaaggc
      961 caacgtcgtc gccatgtcgg ccttgctcac cacaaccatg ccggccatga agaccaccat
     1021 cgaagccctc gaacaagcag gccttcgaga gaaggttaag gtcctcatcg gcggcgcgcc
     1081 gatcacccag aagtatgccg aggaaatcgg tgccgacggc tacagcgaca atgctcccgc
     1141 agccgtcagc ctggccaaga aacagctcgc gctgggcgct tgacgcccct gcgcgaatgc
     1201 ccagaagaaa tccggccacc acggccatga tcggcgcacg gaggcaggat gtcagcctgc
     1261 cggccgcgac tgcgctctta tctggcgcga cggcaatcgc gtgaaccgcg ttgccccagc
     1321 gatctttgac catgccgact ccagccccaa ctccccattc atgaaaccgc tgctcgttcg
     1381 cctgttgcag gacggacccg tcatcaccga cggcgcctgg ggaaccgaac tccaagcacg
     1441 tggattggcc atcggcgatt ttcctgacgt ctggaacctg tcccatccgg atcgggtcac
     1501 cgaggtggcc cgcgcctacg tcgaggcagg gagtcagatc attctcacga acaccttcgg
     1561 ggccaaccgg attcgcatgg ctgaccatgg cgcgccggaa caggtcgcgg aaatcaaccg
     1621 gcgcggcgcg caactctcga ttcaagcggc ggggaatcgg gcatccgtgt tcgcttccat
     1681 cggcccgacc ggaaaactgc tgatggcgca tgaggtgact ctcgatgaag tgcgggcggc
     1741 gttcgtcgag caggcgcagg ccctcgccca gggcggagcg cacggtctcg tcatcgagac
     1801 aatgtcggat ctcgatgagg ccaaggctgc gctcgatgct gcccaacgca ccgggctgcc
     1861 tgtggtcgtc tgcatggtgt tcgacgcggg cagggagaaa gaccgcacga tgacgggcgc
     1921 cacgcccgag caggtggcca gggaattgac aggcgcaggc gcggacgtca ttggtgccaa
     1981 ctgcggcctc gggattgaag gttatgtgcc cgtgtgccga cgactcaaag ccgcaaccga
     2041 tctgccgatc tggatcaagg ccaatgccgg cctgcccaca ttggtcgggg gaaacgtggt
     2101 ctaccagacc acgcccgacc aattcgccag ccacgtgccg gctctgatcg aggcgggggc
     2161 cagcttcatc ggcggctgct gcgggaccaa tccgtcattc attcgggccg tgagagatgt
     2221 gaggggtccc ctgccgtccg cggagcgtct cattgctcgt tcgactcgag gcgaaccggg
     2281 gcatccggcc acgtgaacgt gaacaccgaa ccttcgcccg gagccgaatc cagcgtaatc
     2341 tcgccaccct tggcctccag gattctcttc accagggcca ggccaatccc cgtgccgtca
     2401 ttgtgcgggc cgacacgcag acgttcaaac aaaccaaaca cctgggcatg ataccgcggt
     2461 tcgatcccag gcccgttgtc cgccaccgca aattgccagg cgttacgcag gcgtgtcgca
     2521 cgcaccgtga taacacccaa cggcttgtcc atgaacttga tggcgttgtc gagcaggttc
     2581 tggaatatcc gctgaagctg ccccgccacc ccgcgcaccc gcggaaggct cgatgggatg
     2641 tgaacctgga tgttcgaggg cggcgccagc cctgtcaaca cccgacgcac caaggcgttg
     2701 aggtcaactc gacctccggc tccttggtct gccccaggcg cgtgtaagaa agcacgccct
     2761 cgatcaggtc atgcatgtac tgcacccgct gacgaagcaa accaagcaaa ttgtgtccgt
     2821 tggcgtcaat ctggctcgcg cagtcgctgg caacccagtc cgccaaatga ctgacagctc
     2881 gaagcggcgc cttcaaatcg tgcgcaacca catgcacgaa ttgtcccagt tccatgttcg
     2941 ccgcctgaag ctgagcggtg cgctccttca ccttccgctc gaggtcttca tgcgcccgac
     3001 gcaaagcgct ttccagacgg cggcgctccg tgatgtcctc ggatacgccg atgcacttgc
     3061 ccacggcccc gcccccttcg atgacaggca cgatccgcgt gcgcacataa caggtccatc
     3121 ccgcgcccgt gttggtccga aactcctcct gaaactcaca cccgtgacgc accgcggcct
     3181 tccaggcgcc aacaacccgc tcgcgatccc cagggtccac catctgccac cacacgccgc
     3241 gcggcgccgc gatgtggctc agacgcgcaa tctcccgcca tcgcgcgttg gtgcggatca
     3301 ggcggcctgc gccgtcggtc tggaagaagc caacgggcgc gtggacgctg agcaggcgca
     3361 acaactcgtc ccgttcgcgc accatcgcct cgagcttggc aatgcgcgcc gcggaggcct
     3421 gttccagccc cgattcgatg cgcaggctga cgagtccctc ttcgatgtca gaatgcaccg
     3481 tgagaaccgt ttcggcctgc attgcgctcc caagcatgcc cacaccccat gtatcgcctc
     3541 aggccccaaa tcgtgcaagc cctcaatgcc gccgatcgga cttcataaaa cagactagct
     3601 attgattgtg cgtctcgttt attatgcccc ttggctcagg aatgtgggaa gtcaacgttt
     3661 atgagtgcgg accgcctctt ctcccagccg gcggcaggtg agaacccgat cgcggttgcc
     3721 gggccgatcc aagcgtcccc ggcagcgaat tcgagtgtgg accggctcaa ccccggcggg
     3781 gcccggggtc gggacacctt gatcacgtgt cgaaacagcc agggcgtgga aattcgggcg
     3841 acgcccctgc gcttgagccg ttacgaggcg gttttcgagg tctataaccc ctacagcatc
     3901 ctccaactct cggaggtctt gagcgacttc cgcatcacaa tcggcgagcg aattgtctac
     3961 gccggacgag ccgtcgtcag caaactggtc aacgccagca tcatggtgat ctgcgaggtc
     4021 agcctcgagt cggactggct cgatgtcgag gcgctctcgc cactggctga cccggaatgg
     4081 cttcgccgcg aattcgccgc gttcctgacc gactggagca agattcaagc cgtctctccg
     4141 gccttcaagg cggcggtcgt cgacatggag acgttccttg cagacctccg ccggtggctc
     4201 gatcagatcg agttgggcat ccggtcgcat ccatcccacg aagtcgaaaa gatggagcgg
     4261 gaggtgatgg gccggttgag cggcacggtg ctcgacccaa tcaacgagct gttcagtcgg
     4321 ttcgaggaac tcgcgaaatc catcgcgccc gaccatcacc cgatccatcg cacctacgcg
     4381 cgccgacaac tgcatcccct ggtcctctgc gccccgttcc cgcatcggac cttccagaag
     4441 ccactcggtt acgccgggga ttatgagatg gtcaacatga tcctcaacga ccccttcgag
     4501 ggcgcgtccc tgttcgccaa gtcagtcaat tactggttcc tcatgcaggc gcccgccgcg
     4561 gctcatcgga accgcatcga gtacctgttc caaacgcttg tccgcgaagt ccagagggtt
     4621 gccggcgcgg ggcgtcgcgc ccggatattc aacctcggct gcggccccgc cgcggaggtc
     4681 cagcggttcc tcgagacgca atcactctcg gatgccgccg atttcaccct ggtcgatttc
     4741 aatgaggaca ccctcagctt cgcgcgccac cggcttgcag agatcaagtc ccggaccagg
     4801 cgcagcaccg acttccaatt catccaacag tccgttcacc aggtcctgaa gcgttcggcc
     4861 cggggcgaca agccctccgc gcaatacgat ctggtctact gcgcggggct cttcgactac
     4921 ttgtcggacc gcatctgcca gaggctcatg caatacttcc acgagatgct ggcgccaggc
     4981 gggctcttga tcagcaccaa cgtggatcct tcgaatccaa accgccatgg gatggagtac
     5041 ttgttggaat ggcatttgaa ttatcgtgac gaaaccgtga tgcgcacgtt gcacccaaca
     5101 gccgcccgcc ccgagtgcat aaccaccaaa gccg
//
LOCUS       DAOQRO010000266         5118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_16979_length_5118_cov_4.746000, whole genome shotgun sequence.
ACCESSION   DAOQRO010000266 DAOQRO010000000
VERSION     DAOQRO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16979_length_5118_cov_4.746000"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..960
                     /locus_tag="PKK20_09980"
     CDS             205..960
                     /locus_tag="PKK20_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00257.1"
                     /translation="MLLKVSLVVAILASIATLVISHLQVATGITSLKDTLSQTQGDLA
                     RTQEELDGAKKDARTARDEAEKTARDLDETRSNLEVATANWKQQQERADKFEADWKKA
                     SGLLNDSQRELAAWGALAIPVEQVQNRLAELAKLRETTEALNEENKVLNRKVNELASR
                     IAVYESDKEVAPPLPPGLRGTVVGVDPQWDFVVLDIGGDQGLVERGEMLVNRNGKLVA
                     KVRITTVEPQRAIANVLPEWKQADVIPGDVVMH"
     gene            965..1144
                     /locus_tag="PKK20_09985"
     CDS             965..1144
                     /locus_tag="PKK20_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00258.1"
                     /translation="MAARTILRRGLAMVALAMGLLASICGCSTTNDAENRSERPWNAP
                     KSWEGGMPSGMFEGR"
     gene            complement(1199..2767)
                     /gene="rny"
                     /locus_tag="PKK20_09990"
     CDS             complement(1199..2767)
                     /gene="rny"
                     /locus_tag="PKK20_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNV00259.1"
                     /translation="MNLMAVIDWIQGVLGFLLGIGIGFLIWRWRCLAREKACASQQAE
                     RLEEAQRQSETLIREARLAAQEEAIRLRQEAEDALATRRQECDGWQQRLAEREQLVNQ
                     QLAGLVERERSLREHQREVEERQAKLKAQQSELNGLLVEQREQLQRTAHLSEAEARAA
                     LLKQVERDSLKDAADLSRHVLEEARTRAEDQARRILGLAIQRYAGDHTSETSTATVAL
                     DGADLKGRIIGREGRNIRAFEAATGVTVLIDDTPNAVVISGFDPVRREVARQAMEHLI
                     LDGRIHPTRIEEVVAKVTKEIEETIARAGEDAVFKVGLPPIHPELSNLLGRLRFRYSF
                     SQNVLDHSIEVAHLAGLIAAELGLDPALAKRAGLLHDIGKAVNHEVEGSHALVGADLV
                     RRCGESPEVVEAVAAHHDEVASNSLLAIVLSAADTISASRPGARSESMTTYLKRLENL
                     EQIGGDFPGVEKCYAVQAGRELRVIVQPGTIDDDQAQLLARDIARKIENNLHYPGQIR
                     VTVVRETRCVEFAK"
     gene            complement(2770..3363)
                     /locus_tag="PKK20_09995"
     CDS             complement(2770..3363)
                     /locus_tag="PKK20_09995"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNV00260.1"
                     /translation="MNTESCATKALLRRRIRARNAEVRGEARVEMSRLACGVLRSQPE
                     WNRAKCVLMYYPLPDELDLRPVLREALAAGRVVALPRYQADRGEYEAAIIQDEGRDLH
                     PGWAGIPEPRPSCPPVALKRLDLALVPGLGFDRGGRRLGRGKGFYDRLLVSVHGIRCG
                     VAGEWQVLGAVPAEPHDEPVDCILTPLRWICCRPRTV"
     gene            complement(3360..4793)
                     /locus_tag="PKK20_10000"
     CDS             complement(3360..4793)
                     /locus_tag="PKK20_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNV00261.1"
                     /translation="MSGDDLFTQEPRHAEQALSVESAPSPQTAPLAARMRPRDLAEYV
                     GQRHILGAGQLLRRAIEADRIQSLIFFGPPGTGKTSLAQVIARHTRSRFVRLSGVESN
                     VADVRRELAGAGNRLTNSGQRTILFIDEIHRFNKAQQDVLLPDVESGVVRLIGATTHN
                     PFFFVNAPLVSRSQVFELEPLSESDLLGLLRTALTDSERGLGMLRIRADEDALCHLAK
                     VADGDARKALNALEIGALTTPAGDDGSVHLTREVSEQSIQRKAVVYDADEDGHYDTIS
                     AFIKSMRGSDPDAALYWLAKMIHAGEDARFIARRIVICAAEDVGLADPMALVLALAAF
                     QASEFIGWPEARIPLAEATVYVATANKSNSAYMAIDAALEDVRSGRTLTVPQHLRDAS
                     YGGAKRLGHGVGYKYAHDYPGHFVPQDYLGAARRYYQPTDQGIEKKIKERVASWRASL
                     DQARARPAKPAPGPETAPDSSVSEHRP"
     gene            complement(4915..>5118)
                     /locus_tag="PKK20_10005"
     CDS             complement(4915..>5118)
                     /locus_tag="PKK20_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00262.1"
                     /translation="THVRQNRRRVNNYEPVTLYNCNLSALRICLIALKADLHPEPSWP
                     ALQERCQHNSQIPFQILVNRRSK"
BASE COUNT          980 a         1718 c         1494 g          926 t
ORIGIN      
        1 aattcgttaa gtccctcttc ctcaatcacc ccatcccagc tcagaaccgc cctggtcaga
       61 caaccgcggc cttgcacgtt tcctgctggc attgaataag gaccggcagc cggtctgccg
      121 cccgggaacg gatttcacac gagcgttgac accgggggcg gcgatgatta gcttgccgcc
      181 ggttcgcaaa accagactag aaacatgtta ctcaaagtca gcctcgttgt tgccattctg
      241 gcgagcattg ccaccttggt gatcagtcat ttgcaggtcg ccaccggcat tacctcgttg
      301 aaggatactc tcagccagac ccagggcgat ctcgcccgta cccaggagga actcgatggc
      361 gctaagaaag acgcccggac cgcccgggac gaggccgaga agaccgcccg cgacctcgat
      421 gaaacccgat cgaacctcga agtcgccacc gcgaattgga aacagcaaca ggagcgcgcc
      481 gataagtttg aagcggattg gaagaaggcg tcgggtctcc tgaatgattc tcagcgcgag
      541 ttggccgcct ggggcgccct ggccatcccg gtcgagcagg tgcaaaaccg cctggcggaa
      601 ttggccaagt tgcgggaaac gacagaagcc ttgaacgagg aaaacaaggt cctcaaccgc
      661 aaagtcaacg aactcgcatc caggattgcc gtctacgaaa gcgacaagga agtggcgccg
      721 cctcttcctc ccggtctccg gggcaccgtg gtcggcgtcg atccgcagtg ggacttcgtc
      781 gtgctcgata tcggcggcga ccagggcctg gtggaacggg gtgagatgct cgtgaaccga
      841 aacggcaagc tcgttgcgaa ggtccgtatc acaacggtcg agccccagcg cgccatcgcc
      901 aatgtcctgc ccgagtggaa acaggccgat gtgatccctg gcgacgtggt catgcactga
      961 gcttatggcg gcccgaacga ttcttcgacg cggtttggcg atggtcgctc tggcgatggg
     1021 attgctggcc tcgatctgcg gttgttccac caccaacgat gccgagaacc gctccgagcg
     1081 cccgtggaat gcgcccaaga gctgggaggg cggcatgccc tcgggcatgt tcgaggggcg
     1141 ctgatctcgg cggaagacgc ctccaagcac cgccggcctc ggaccgcggc gaggcggttt
     1201 atttggcgaa ctcgacgcat cgggtctcgc gcacaaccgt gacccgaatc tggcctggat
     1261 agtggaggtt gttctcgatc ttgcgggcaa tgtcacgggc cagaagctgc gcctgatcgt
     1321 cgtcgatcgt acccggttgc acaatcacac gcagttcgcg cccagcctga accgcataac
     1381 atttctcaac gcccggaaaa tcgccgccaa tttgttcgag gttctcgagc cgcttcagat
     1441 aggtggtcat cgactccgag cgcgctcccg ggcgggacgc gcttatggtg tcggccgcgc
     1501 tgagcacaat ggcgagcagc gaattggatg cgacctcgtc gtgatgggcg gccacggcct
     1561 cgaccacctc gggcgattcg ccgcaacggc ggacgagatc ggcgcccacc agtgcgtgcg
     1621 agccttcgac ttcgtggttg accgctttgc cgatgtcgtg gagcagcccg gcccgcttgg
     1681 ccagcgccgg atccagcccc agttcggctg cgatgagccc ggccaggtgc gccacctcga
     1741 tcgaatgatc gaggacattc tgcgagaaac tgtaccggaa cctcagacgc cccaggagat
     1801 tggaaagctc cggatggatc ggcggcagcc cgaccttgaa caccgcgtct tcaccggcgc
     1861 gggcgatcgt ctcctcgatc tctttggtca cttttgccac aacctcctcg atgcgcgtcg
     1921 gatgaatgcg cccgtcgaga atcagatgct ccatggcttg ccgggcaacc tcacgccgca
     1981 ccggatcgaa accggaaatc acaaccgcat tgggcgtgtc gtcgatcagc accgtcaccc
     2041 ccgtggctgc ctcgaaagcc cgaatgttcc gtccctcgcg cccgatgatt cgccctttca
     2101 gatcggctcc atcgagagcc acggtggccg tgctggtttc agatgtgtgg tcgccggcgt
     2161 agcgctgaat ggcgagaccc aggatgcgcc tggcctggtc ctcggcccga gtacgcgctt
     2221 cctcgagaac atgccgcgac aggtccgccg catccttgag tgagtcgcgt tcgacctgct
     2281 tgagcagcgc cgcacgggcc tcggcctcgc tgaggtgggc cgtgcgctgg agctgctccc
     2341 gctgttcaac caaaagcccg ttcaactcac tctgctgcgc tttcaacttg gcctgtcgct
     2401 cttcaacctc ccgctgatgc tcgcgcagac tccgttcccg ctcgaccaga cccgccaact
     2461 gctgattgac cagttgctcg cgctcagcca gccgctgttg ccatccgtcg cactcctgcc
     2521 tccgggtcgc cagcgcatcc tcggcctcct ggcgaagccg gattgcctcc tcctgggcgg
     2581 ccaggcgggc ctcgcgaatc aacgtttctg actgccgttg agcttcttcc agccgctcgg
     2641 cctgctgtga agcgcaggcc ttttctcgcg ccagacaccg ccatcgccag atcaaaaaac
     2701 cgatgccgat gccaaggaga aaccccaaaa cgccttggat ccaatcaatt accgccatca
     2761 gattcattgt caaaccgtgc gcgggcggca acaaatccag cgcaacggcg tcaggatgca
     2821 atccaccggt tcatcgtgcg gttctgccgg cacagcgcca agcacttgcc attcaccggc
     2881 aactccgcaa cggattccat gcacggacac aagcagccga tcgtaaaacc ctttgcctcg
     2941 acccaaccgg cgcccgccgc ggtcgaatcc tagcccaggc accagcgcca agtccagtcg
     3001 ctttaacgca accggcgggc atgaaggcct gggctcggga atccccgccc agcccgggtg
     3061 taggtcccgt ccctcatcct ggatgatcgc cgcttcgtac tcacctcgat cggcttgata
     3121 ccgcggcaac gcaaccaccc gcccagccgc cagcgcctcg cgcaagaccg gacggagatc
     3181 aagctcgtcc ggcagcggat agtacataag gacgcatttc gcccgattcc attccggctg
     3241 gctccgcaaa actccgcacg ccaaacgcga catctcgacc cgtgcctccc cgcggacttc
     3301 ggcattgcgg gcgcgaatcc gtcgtctgag taacgcttta gttgcgcaag attccgtgtt
     3361 catggacggt gctcggacac tgaagagtcc ggggccgtct cgggccccgg tgcaggctta
     3421 gccgggcgag cgcgcgcttg atcgagcgaa gcgcgccagg aagcaacgcg ctctttgatc
     3481 ttcttctcga tgccctgatc ggtcggctgg taatagcgcc gcgccgcgcc caggtaatcc
     3541 tggggaacga aatgccccgg ataatcatgg gcgtacttgt aaccaacacc gtggcccaga
     3601 cgcttcgccc caccgtaact ggcgtcgcga aggtgctgcg gcaccgtcag ggtccgtccg
     3661 gatcgcacgt cttcgagcgc tgcatcgatt gccatgtacg cgctgttgct cttgtttgcc
     3721 gtcgccacat aaaccgtcgc ctcggcaagc ggaatccggg cttcgggcca cccgatgaac
     3781 tcggaggcct gaaacgccgc cagcgctaag accaaagcca tcggatccgc cagaccaaca
     3841 tcctccgccg cgcaaatcac aatcctccgc gcaatgaaac gcgcatcctc gcccgcatgg
     3901 atcatcttgg ccagccagta caacgctgcg tccggatcgc tgccccgcat cgacttgatg
     3961 aacgccgaaa tggtgtcgta atgaccgtcc tcgtccgcgt catacacaac cgccttgcgc
     4021 tggatgctct gctccgacac ctcccgcgtc agatgaacgg acccgtcgtc gccggcaggc
     4081 gtggtcaacg ctccgatctc gagcgcgttc aaagccttcc gcgcgtcgcc atccgccacc
     4141 ttcgccaaat gacacaacgc atcctcgtca gcccgaatcc gcagcatccc cagaccgcgt
     4201 tccgaatcgg tcagagccgt ccggagcagg ccgagcagat cggattcgct cagcggctcg
     4261 agttcgaaca cctgtgatcg cgacacgagc ggcgcgttga cgaagaagaa cggattgtgc
     4321 gtcgtggcgc caatgagccg aaccaccccg ctctcgacgt ccggcaacaa gacatcctgt
     4381 tgggccttgt tgaaccgatg aatctcatcg atgaatagaa tcgtccgctg accgctgttc
     4441 gtgagccgat tccccgctcc cgccaactcc cgccgcacgt ccgcgacgtt ggattcgacc
     4501 ccgctgagcc gcacgaacct ggaccgggtg tgccgcgcaa tcacctgcgc caaagaggtc
     4561 ttccccgtcc ccggcggccc aaagaaaatc agcgattgaa tccggtccgc ctcgatcgcg
     4621 cgccgcagga gctggcccgc tcccaagata tgcctctgcc ccacatactc ggccaggtcc
     4681 cgcggtcgca tgcgcgccgc aagcggcgcc gtctggggtg aaggcgcgga ctcgacggaa
     4741 agcgcctgtt cggcgtgccg cggttcctgc gtgaacaaat cgtcaccaga catgccgcca
     4801 ttctaacgca gcgctgccca gccgcaacca aagccgcgcg gcgcggaaat ggcctttcgc
     4861 gcctttccgt gtcgagggac gatcgagacc ggcgccccgg cgcccacggt cttttcattt
     4921 ggagcggcga ttgacgagta tctggaaagg gatttgggaa ttgtgctggc agcgctcctg
     4981 cagggccggc caagaaggct cggggtgaag gtccgccttg agcgcgatca ggcagatgcg
     5041 cagggccgag aggttgcagt tatataaagt gacaggttcg taattattga cacgccttct
     5101 gttttggcgt acatgtgt
//
LOCUS       DAOQRO010000267         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17033_length_5107_cov_5.014252, whole genome shotgun sequence.
ACCESSION   DAOQRO010000267 DAOQRO010000000
VERSION     DAOQRO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17033_length_5107_cov_5.014252"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKK20_10010"
     CDS             complement(<1..257)
                     /locus_tag="PKK20_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00263.1"
                     /translation="MSLYDTDVRVIVRGKAGAKVEFGNRALIGENRQGVILDHQLFQE
                     SAPADSQLLFGSLLRVWEGTGRHVGSRRATVVFDRGGFSPAL"
     gene            complement(244..1050)
                     /locus_tag="PKK20_10015"
     CDS             complement(244..1050)
                     /locus_tag="PKK20_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00264.1"
                     /translation="MKGWSVAAKRAVSAREQIKFQQRSRRALRCTILHTLLQEDYRGF
                     SCQLASNPLYQWFCGIDALDAVRVPSKSQVQRFAHWLPAGEMRSVIEGLLRGAIEKPS
                     KLGLREALDLEEYLLDSTCLKANVHFSADWVLLRDGVRTLMKATLLIRKAGLRRRMVP
                     TRGVSAADESAEHSDVPADAADGQQEGAQASAAPDEEAGGCGARTCAAASGIAGPTLG
                     ADRVDATAGRPNLALARRGAGALAPGVRAGARADHRRAARGQRGEDLESL"
     gene            complement(1047..1205)
                     /locus_tag="PKK20_10020"
     CDS             complement(1047..1205)
                     /locus_tag="PKK20_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00265.1"
                     /translation="MPCSIIPFPQELRPQLPTIAGNVDYLTLRQHLEQIDILLCDGAW
                     SEISSNGL"
     gene            complement(1848..5051)
                     /locus_tag="PKK20_10025"
     CDS             complement(1848..5051)
                     /locus_tag="PKK20_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNV00266.1"
                     /translation="MTIDRPLDGADGLIPWLRRLHTIDDPTSRAGTRRSGDCRPHAIG
                     SALLGNSRLSAVGSFVLLFCAAWTLGAVASETPAIRVWPVDPLVKVFPDDPVGAECAA
                     RAEVARGEHASLQVVVRADTPIRSLRAAIRSLDASDGSGRLDAMPARFVGYVPVDRPT
                     QKPSQDQLRKPPADYPDPLLELESIDVAAGRAQAVWITVPIPLSAVPGMYRGALEITG
                     VVAEKPVRLTQRLEIQVYPASIARSRLWVTDWFAMQWRHLEVSPQPESEDYYALLRRY
                     ARNLAEHRHNVALISPLNLATFTVGPGGGLGIDFARFDRWVNIFREEGVIGRIEGGHI
                     GGRKAGWDSDFVVTIHRVEDGKVVSASVDPAGADAERFYAQFFPALVAHLKERGWLSD
                     YLQHLADEPTASNLGSYRAMAALIRKHAPELRVIEACHTKDLVGAIDVWVPQLNFLHD
                     DFAHYTTRRRAGDEVWFYTCVFPQGEYANRFIEQPLIKTRLLHWINFRYGVTGYLHWG
                     YNHWTENSPFTHTTRPHGGPPYLPAGDPWIVYPGKDGPLDSIRFEAMRDGIADHELLS
                     LLAQRDPEAAQQIAGRHVLAFDRYNTDVVAFRAARRELIERLSDRVSMPEPSASVTKA
                     PEAGLEVELTAIGDWDVRVTVTRREPGQSAARTNTVVRIAPPEAVEVAAERHDSLPLF
                     NPNTGGWMKGARLRLVQAQETTTPFLVDPGSVVLRAGPDLNAPVFKPRIDYDADLVWG
                     TIGRTAQGGIREGQPVYAAYRCYALRLDALVLRPDGTIDVRRGEPRAAAPKPGPVRAG
                     EVHLANIWLPGRVARLGSANLLPIRETSYPEPALLRPTPIEQFLPKTLARLETGQLLR
                     VLAWGDSVTDGSYLSGPDRERWQAQFVERLQAAFPKARIELVTEAWGGRNTASYLAEP
                     PGAPHNYREKVLGAQPHLIVSEFVNDAGLDAAQVEERYGRLLEDFSAIGAEWIILTPH
                     YVRPDWMGLDRECDIDNDPRPYVAGLRQFAGRHGVALADAARRYGRLWRQGIPYTALM
                     LNSINHPDADGMKIFADALMALGWTPETPSTPGLSSQ"
BASE COUNT          936 a         1690 c         1585 g          896 t
ORIGIN      
        1 agggccggac tgaaaccgcc ccggtcgaag accacggtgg cccgtcggga gcccacatga
       61 cgcccggtgc cctcccacac ccgcagcagg ctgccgaaga gcaattggct atcggccgga
      121 gccgactcct gaaacagttg gtgatcgagg atgacccctt gcctgttctc cccgatcagc
      181 gccctgttgc caaactccac tttcgctccc gccttcccgc gcacgatcac tcgcacgtcg
      241 gtgtcataga gactcaagat cttctccgcg ttggccacgg gccgcccgcc gatgatccgc
      301 tcgtgcgcct gctcttacgc ccggagcaag agctccagca ccccgtcgag ccagcgcaag
      361 atttggtcga cctgcggtcg cgtccactct gtctgctccc agtgtcggtc cagcaattcc
      421 cgatgccgcc gcgcaggtcc gcgcaccaca cccaccagct tcttcatctg gcgcagcact
      481 cgcttgcgcc ccttcttgct gcccgtccgc cgcgtctgct gggacatctg aatgctcagc
      541 cgattcatcc gccgcagaaa ctcctcgggt gggcaccatg cgtctgcgca ggccagcctt
      601 acggatcagg agcgtggcct tcatcaacgt gcgcacccca tcccgcaaca ggacccaatc
      661 ggccgagaag tggacgttgg ctttgaggca ggtgctatcc aagaggtact cctccaggtc
      721 cagcgcctcc cgcaggccca gcttgctggg tttctcgatc gcccctcgga gcagaccctc
      781 gatcaccgag cgcatttcgc ctgccggcag ccaatgggca aagcgttgca cttggctctt
      841 ggagggcaca cggaccgcgt cgagcgcatc gattccgcag aaccattgat agagcggatt
      901 gctcgccaac tgacaactga acccgcgata gtcctcctgc aacaaggtat gcaggatcgt
      961 gcagcgcagc gctcgacgcg agcgctgctg gaacttgatt tgctcacggg cgctcacggc
     1021 ccgcttggcc gccacgctcc atcctttcaa agcccgttgg acgaaatctc gctccacgcc
     1081 ccgtcgcaca aaagaatgtc gatttgttcg aggtgttgac ggagagtcag gtagtcaacg
     1141 ttcccggcaa tggtggggag ctgcgggcgc agttcctgag ggaacgggat gatggagcac
     1201 ggcatagagc tagtctgata gatagtttat ctcgtgccaa cttgaccgtc cattcaatca
     1261 aaagacaatg ccaacctcct ttcctttgcg cgttcaagcg attcgttcgc gcggacagca
     1321 accccggctc tatgtcagtt tcccgctggc cttggccgca gccatcggcc tggagccaga
     1381 cgaagaagtg caatcgaaac tgctcgaccg cggcgagatt catttgttgc gcaccaaccg
     1441 tccggatccc tcgactccaa atccagggtg cgcaagtaag cggctgcccc catcatagag
     1501 gcacgatttt cggctctttg gagaaatcca ccccctatct cgagcctccc tcagccctcg
     1561 gcatcaagcc ctatgcaccc ccgctcactt ctggacaggc tctactctgg cgctgcagcc
     1621 atccccaagg tcggggcggg ggagaatccc ttcccggcgc cgatacatca ggtggccatt
     1681 tcgcattcac tggaacgctc atccttcgac tcgagcccct ctcccacctg cctgcgccca
     1741 ggcttccgca ggcacgtcgg atgggagaag gccggggtga agggtctgtg tatggtatcc
     1801 tgccgactag acagtcagac tcgacgaagc tctgtgaatc aaccgggcta ttgactggaa
     1861 agcccgggcg tcgacggtgt ttccggtgtc catcccaacg ccatcagggc gtcggcgaaa
     1921 atcttcattc cgtcggcatc ggggtggttg atcgaattga gcatgagggc cgtgtacggg
     1981 atgccctggc gccaaaggcg cccgtagcgc cgggccgcgt cggccagggc gacgccatgt
     2041 cgcccggcga actgccgcag cccggcaacg tacggtctcg ggtcattgtc gatgtcgcat
     2101 tcgcgatcca aacccatcca gtcgggccgc acgtaatgag gcgtgagaat gatccactcc
     2161 gccccgatgg cgctgaagtc ctcgagaagc cgtccgtagc gttcctcgac ctgggcggcg
     2221 tccagaccgg cgtcattgac gaattcggag acgatgagat gcggttgggc gccgaggact
     2281 ttttcacggt agttgtgcgg agcgcccggc ggttccgcaa ggtaactcgc cgtgttgcgt
     2341 ccgccccacg cctcggtcac cagttcgatg cgcgctttcg ggaacgccgc ctgcaaacgc
     2401 tcgacaaatt gcgcctgcca gcgctcgcgg tccggaccgg atagatagct gccgtcggtg
     2461 acgctgtcac cccaggcaag aacccgcagc aattgaccgg tctcgaggcg cgcgagtgtc
     2521 ttgggcagga attgctcgat gggtgtcggc ctcaaaagcg ccggctcggg atagctcgtc
     2581 tcgcgtattg gcaggagatt ggccgaaccg agcctggcca cgcgtccggg cagccagatg
     2641 ttcgccaggt gaacctcgcc cgcacgcaca ggccccggct tgggcgcggc tgcgcgcggc
     2701 tcgcctcgcc gcacatcgat ggttccgtcg ggccggagca ccagggcgtc caaccgcagc
     2761 gcataacagc ggtaggccgc gtagacgggc tgtccctccc gaatgccgcc ttgggccgtg
     2821 cgcccgatcg ttccccaaac caaatcggca tcgtagtcga tccgcggctt gaagacaggc
     2881 gcgttcaagt cgggtcccgc gcgcaagaca acgctccccg gatcgaccag gaagggcgtg
     2941 gtagtttcct gcgcctggac cagccggagt cgggcgccct tcatccatcc gccggtattc
     3001 gggttgaaca gaggcagcga atcgtggcgc tcggcggcca cctcgacggc ctcggggggc
     3061 gcaatccgca cgacggtgtt ggtgcgagcc gcggattggc ccggctcacg ccgggtcact
     3121 gtgacgcgga catcccagtc ccctatcgca gtcagttcca cttcgagacc ggcctcgggc
     3181 gctttagtca ctgaagccga cggctccggc atcgaaacgc ggtcggacag cctctcgatc
     3241 aactcgcgcc gggccgcgcg gaaggcgacc acatcggtgt tgtagcggtc aaacgccagc
     3301 acatggcgtc cggcgatctg ctgcgctgcc tcgggatcgc gctgggccag caggctcagc
     3361 aactcgtgat cggcgatgcc gtcgcgcatg gcttcgaagc ggatcgaatc gagcgggccg
     3421 tctttgccgg ggtagacaat ccagggatcg cctgcgggca agtagggcgg gccgccatgg
     3481 gggcgtgtgg tgtgggtgaa ggggctgttc tccgtccagt gattgtagcc ccagtgcaga
     3541 tacccggtca ccccatagcg aaagttgatc cagtgcagca gccgggtctt gatcaaaggc
     3601 tgctcgatga agcgattggc gtactcaccc tggggaaaga cgcaggtgta gaaccagact
     3661 tcgtcgcccg cccgccggcg ggtcgtgtag tgcgcgaaat cgtcatgcag aaagttcagt
     3721 tgcggcaccc agacgtcgat cgccccaacc aggtccttcg tgtgacaggc ctcgatcacc
     3781 cggagttcgg gtgcgtgctt gcggatcagg gccgccatcg cgcggtagct gcccaaattc
     3841 gaggctgtcg gctcgtcggc caggtgctga agataatcgc tgagccagcc gcgttccttc
     3901 agatgtgcca ccagtgcggg aaagaattgc gcatagaagc gctccgcatc ggccccggcc
     3961 ggatcgacgc tggcggaaac caccttgcca tcctcgaccc gatggattgt gaccacgaaa
     4021 tccgaatccc atcccgcctt gcgcccgcca atgtggcccc cctcgatccg gccaatcacg
     4081 ccttcctccc ggaagatgtt cacccaacga tcgaatcggg cgaagtcgat gccgagcccg
     4141 ccaccggggc cgacggtgaa tgtggccagg ttcagcggtg agatcagagc gacattgtgg
     4201 cggtgttcgg ccaggttccg cgcgtagcgc cggagcaggg cgtagtaatc ctcggactcg
     4261 ggttgcggcg agacctcgag atgccgccac tgcatggcga accagtcggt cacccacagc
     4321 cgcgatcgcg cgatcgatgc cggatagacc tgtatttcga gccgctgagt caggcggacg
     4381 ggtttctcgg caacgacacc cgtgatctcg agcgccccgc gatacatgcc ggggaccgca
     4441 ctgagcggga tggggaccgt gatccatacc gcctgggccc ggccggccgc aacgtcgatc
     4501 gactcgagtt caagcagcgg gtccggatag tcggcgggcg gcttgcgcag ttgatcctgc
     4561 gacggtttct gcgtgggacg atcgactggc acatacccga caaaccgcgc gggcatcgcg
     4621 tccaaccgac ccgaaccgtc agacgcatcc agagaccgga tcgccgcccg caaggatcgg
     4681 atcggcgtgt cggcacggac gaccacctgt agcgatgcgt gttctccccg cgccacctcg
     4741 gcccgggcgg cgcactcggc gccaacggga tcgtccggga agaccttcac cagcggatcg
     4801 accggccaaa cgcgaatcgc gggcgtttcc gacgccacgg cgccgagggt ccacgccgcg
     4861 cagaacagca ggacaaatga ccccaccgcg ctcagacgcg agtttcccag cagggcgctg
     4921 ccaatcgcgt ggggcctgca atcgcccgac cgtcgagtcc ctgcgcgtga agtggggtcg
     4981 tcgatcgtat gcagccgccg aagccagggg atgagtccat cggcgccgtc gagtggtctg
     5041 tcaatcgtca tgcagagaga gtagagaggt gaaggcggaa tggaacaacg aatgtggcaa
     5101 acaccac
//
LOCUS       DAOQRO010000268         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17036_length_5107_cov_3.579375, whole genome shotgun sequence.
ACCESSION   DAOQRO010000268 DAOQRO010000000
VERSION     DAOQRO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17036_length_5107_cov_3.579375"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKK20_10030"
     CDS             complement(<1..333)
                     /locus_tag="PKK20_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00267.1"
                     /translation="MKHHTRQRVPWFVVQLLAATLLTGMATAFYTLRLNPEIAYLRGS
                     DRIKRAWARKMTLEHGAKTILFGGSSCEFSIDPEQLLERHGLPAVNLGRNAGLGASVL
                     IEATLIETR"
     gene            complement(335..1738)
                     /locus_tag="PKK20_10035"
     CDS             complement(335..1738)
                     /locus_tag="PKK20_10035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNV00268.1"
                     /translation="MNFADSRFWILLLSGLAVIALLRIPAGAWLGSRAAVFDRLALIA
                     LGLCLLMAVSWVTCVIFLVVAVFSYCAMCYLVRRGRRHSRRLLILFVPLQLLPLLYFK
                     YADFILNETLGGSFDTLRGLIIPAGISFYTFQMMSFVIDTLAFGRPLPRVLDFFNFAG
                     FFPQVVAGPIERREDLLPQMEQFKFRWSTENLNAGAAWVAVGMFFKCCLADNAAIYVD
                     RTSVSNPFSIWLANLMFGLRIYYDFAGYSLVAVGLGRCLGIRLTLNFLSPYCSTSIRE
                     FWHRWHVTLSGWFRTYVYLPLGGGRTRWWMFNVAVVFVVSGLWHGAGWGFLLWGTFHG
                     IALIVNRALGGAIRLPRFVAWVVTLVAVLFGWVFFYETRTPDLLTKVATLWNPMAYTA
                     AAFRETLTSMSGGHLFALTCLLLLVAVVWAVEWLSVARRDDPYCYLRHPVAAVVLVVL
                     TVLLAPGNDNAFIYFAF"
     gene            complement(1735..1926)
                     /locus_tag="PKK20_10040"
     CDS             complement(1735..1926)
                     /locus_tag="PKK20_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00269.1"
                     /translation="MAKSIALKLKVDDRTPDFEARFCRSPDDGLRKALDRSPHISSFL
                     ERKGFDCGFAGLRNIRAKA"
     gene            2049..2909
                     /locus_tag="PKK20_10045"
     CDS             2049..2909
                     /locus_tag="PKK20_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNV00270.1"
                     /translation="MTTSTIPQSLAVCSWSLQPVSAIDLFDKLAATGLNRVQIALDPI
                     RENAGGAWSDFGRLCGERGVTCVSGMFAAVGEDYSTLEAIKRTGGVVPDVHWEQNWRN
                     LQADAEVAKGLGIKSIAFHAGFLPHEESDPAFAKLMDRLQKIADFFAPLGIELALETG
                     QETADTLRTFLIKLDRPNVGVNFDPANMILYAKGDPIQSLRTLGPWLRSCHLKDAIGT
                     KVPGTWGEEVVLGTGQVDWRAFFTVLAELKFAGNACIEREAGNQRAADIRTAREYIQK
                     LLNAMPPAVA"
     gene            2964..3986
                     /locus_tag="PKK20_10050"
     CDS             2964..3986
                     /locus_tag="PKK20_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00271.1"
                     /translation="MTNIGIVGLGFMGLTHIKAYRQLPGANIVAICDAVRLPEDGNLT
                     PVAGNISTADQIKFDMSVVKGYRDYAQMLADPDVQLVDICLPTPLHADVAIAALRANK
                     HVICEKPLARSSSVCRQILDAAKSSKGYFMPAHCIRFWPEWAWAKQAISENRYGKVLA
                     ARLRRVSEPPGWSKATYLKGEDSGGALLDLHIHDTDFVQFCFGRPRRVFSQGFALISG
                     AIDHVVTQYEVDCGAAVSAEGTWVMHAGHGFNMAYTIVFENATADYDLTRGPNALKLF
                     QNDKPAQTITCEGGDGYVGELRHMLESIEAGRPPSIVTGEDALNAVAICEAEEKSIKT
                     KQLVTV"
     gene            complement(4037..4228)
                     /locus_tag="PKK20_10055"
     CDS             complement(4037..4228)
                     /locus_tag="PKK20_10055"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV00272.1"
                     /translation="MGSAYAEYQDHVEGTLTPGKLADVVILDRDIFTIPPTEIDQVRV
                     VCTIMDGRVVFDAEHDSAR"
     gene            4614..4850
                     /locus_tag="PKK20_10060"
     CDS             4614..4850
                     /locus_tag="PKK20_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00273.1"
                     /translation="MSVGDPVVLDLRLGLVAWDDDQSPSMSGLGRRPVELRLPMEARC
                     GFAGVRARARARVMDVPIRLESAVDEGASASGAG"
BASE COUNT         1096 a         1553 c         1571 g          887 t
ORIGIN      
        1 ccgtgtctcg atcagcgtcg cctcgatcaa cacgctcgcg cccaggccgg cattgcgccc
       61 gagattgacc gccggcaggc cgtggcgttc gagcaactgc tccggatcga tcgagaattc
      121 gcagctcgat ccgccaaaca ggatcgtctt cgccccgtgt tcgagcgtca tcttccgcgc
      181 ccaggcccgc ttgatccggt cgctgccgcg cagatacgcg atttcggggt tcagccgcag
      241 ggtgtagaag gccgtcgcca tcccggtgag cagcgtcgcc gcaagaagct gcacaacaaa
      301 ccacgggacg cgctgacggg tgtggtgttt cacactaaaa cgcgaagtag atgaaggcat
      361 tgtcgtttcc cggcgccaga agaacggtca gcaccaccag cacgaccgcc gccaccggat
      421 gccgcagata gcagtacgga tcgtcccggc gggcgactga gagccattcg accgcccaca
      481 caaccgccac cagaaggagc agacaggtga gggcgaacag gtgaccgccg ctcatcgagg
      541 tcaacgtctc gcggaaggcg gccgccgtat acgccatcgg attccagagc gtcgccacct
      601 tcgtcagcaa atccggagtg cgcgtctcgt aaaaaaagac ccacccgaac aacaccgcca
      661 ccaatgtcac cacccaggcc acgaagcgcg gcagccggat cgcgccgccc aatgcccggt
      721 tcacgatgag ggcgatgccg tggaacgtac cccacagcag gaatccccac ccggccccat
      781 gccacaggcc cgagacgacg aaaaccacgg cgacgttgaa catccaccat cgggtgcgtc
      841 ctccgcccaa cggcagatac acataagtcc gaaaccaacc gctgagcgtc acatgccagc
      901 ggtgccagaa ctcccggatg ctcgtcgagc aataggggct gaggaaattc agagtcaacc
      961 ggatgccaag gcagcgtcct aagcccaccg ccaccaggct gtacccggca aaatcgtaat
     1021 agattcgaag gccgaacatc aggttggcca gccagatcga gaacgggttg ctaaccgagg
     1081 tccgatcgac atagatcgcc gcgttgtccg ccagacagca cttgaagaac atgccgacag
     1141 ccacccaggc agcgcccgcg ttcagattct ccgtcgacca ccggaacttg aactgctcca
     1201 tctgcggcag caaatcctcc ctccgctcga tcgggccggc caccacctgc gggaagaacc
     1261 cggcgaagtt gaagaaatcg agaacgcgcg gcagcgggcg gccaaaggcg agtgtgtcga
     1321 tcacaaacga catcatctgg aacgtataga acgagatgcc ggccgggatg atcaggccgc
     1381 gcagggtgtc gaaagacccg ccgagcgttt cgttcaggat gaagtccgcg tatttgaagt
     1441 acaggagagg aagcaactgc aacggaacga acagaatcaa aagccggcgc gaatgccgcc
     1501 tgccccggcg aaccaggtag cacatcgcac agtacgaaaa cacagcgacg accaggaaga
     1561 tcacgcacgt cacccaactg acggccataa ggagacacaa tccgagggcg atcagggcga
     1621 gcctgtcgaa cacggccgcc ctcgatccaa gccaggcgcc cgccggaatg cggagcaacg
     1681 caatgaccgc cagacccgag agcagcagaa tccagaatcg gctgtcggcg aaattcatgc
     1741 tttggctcgg atgtttcgca gcccggcaaa gccgcaatcg aaaccttttc tctcaagaaa
     1801 agaagaaatg tgcggagagc ggtccaaggc tttgcggagt ccatcatccg ggctcctgca
     1861 aaaccgcgcc tcgaaatctg gggtgcggtc atccaccttc aatttcaacg cgatgctctt
     1921 ggccatgtgc ggcgaaggtt ttgattcaac gccaccatga agtcaaccgc cgatcgaaac
     1981 cggaaatcgc ggccgcggtt tggcgcttgt caggctgacg acaaccggta tgatgcctcg
     2041 caattacaat gacgacatca actattcccc aatccctggc ggtctgcagt tggtcactgc
     2101 agccagtgtc ggcaatcgac ctgttcgata aactcgcggc gacgggtctg aaccgggttc
     2161 aaatcgccct ggacccgatc cgtgaaaacg ccggaggcgc gtggtccgat ttcggacgtc
     2221 tttgcggcga gcggggtgtg acctgcgttt cgggcatgtt tgcggccgtc ggcgaagatt
     2281 actcgaccct cgaagccatc aagcgcacag gtggcgtggt gccggacgtt cattgggaac
     2341 agaactggcg caacctgcag gccgatgccg aggtcgccaa aggcctgggg atcaaatcca
     2401 tcgcctttca cgccggattt ctgccgcacg aagagagcga cccggccttc gccaagctga
     2461 tggaccggct tcagaagatt gccgacttct ttgctcctct gggcatcgaa ctggcgcttg
     2521 aaaccggaca ggagactgcc gatacgctgc ggaccttcct gatcaaactc gatcgtccca
     2581 atgtcggcgt caatttcgat ccggccaaca tgatcctcta cgcgaagggc gatccgatcc
     2641 agtcgttgcg caccctcgga ccgtggctgc gatcctgcca cttgaaggac gcgattggga
     2701 cgaaggtgcc tgggacctgg ggtgaggagg tggtgctggg caccggccag gtggattggc
     2761 gggccttctt caccgtgctg gccgagctga agtttgccgg caacgcctgc atcgagcgcg
     2821 aggcgggcaa ccagcgcgcc gcggatatcc gcaccgcgcg cgagtacatc cagaagctcc
     2881 tgaacgcaat gccgcccgct gtggcgtgac cgccgattgg ctgattcgac actgacacaa
     2941 acattcactc gacacacctt tttatgacaa acattggcat cgttggtctg ggcttcatgg
     3001 gcctgactca catcaaagcg taccgccaac tgcccggcgc caacatcgtc gccatctgcg
     3061 acgccgtccg gttgcctgaa gacggcaacc tcacgccggt tgcgggcaac atcagcacgg
     3121 cggaccagat caagttcgac atgtcggtgg tcaagggcta ccgggactat gcccagatgc
     3181 tggccgatcc ggacgtgcaa ctggtggaca tctgcctgcc aacgcccttg cacgccgatg
     3241 tggccatcgc ggctcttcga gcgaacaagc acgtgatctg cgagaaaccg ctcgcccgct
     3301 cgtcgtccgt ctgccgccag attctcgatg ccgccaagtc gtccaagggc tatttcatgc
     3361 cggcccattg catccgcttc tggcccgagt gggcctgggc caaacaggcc atctcggaaa
     3421 accgctacgg caaagtgctt gccgctcgat tgcggcgggt ttcggagccg ccgggctgga
     3481 gcaaggccac ctatctcaaa ggcgaagatt caggcggcgc cctgctcgac ctgcacatcc
     3541 atgacaccga cttcgtccaa ttctgcttcg gacgcccgcg tcgggtcttt tcccagggat
     3601 tcgccctcat tagcggcgcg atcgatcatg tcgtcaccca gtacgaggtg gactgcggtg
     3661 ccgccgtgtc ggccgagggg acgtgggtga tgcatgcggg gcacggattc aacatggcct
     3721 acaccatcgt gttcgaaaac gccaccgccg actacgacct tacgcgcgga ccgaatgccc
     3781 tcaagctgtt ccagaacgac aagccggctc agaccattac ctgcgagggc ggcgacggat
     3841 acgtcggcga actgcgccac atgctcgaaa gcatcgaggc cggacgcccg ccgtccattg
     3901 tcaccggcga agacgcgctg aacgccgtgg ccatctgcga agccgaggaa aagtcgatca
     3961 agaccaagca gttggtcaca gtctagcacg ggtcgatcac aggctgcgcg ctgaatcgga
     4021 ttcgggccgg ttggcgtcac cgcgcggagt cgtgctccgc gtcgaacacc acccgcccgt
     4081 ccatgatggt gcacaccacg cgcacctgat cgatctcggt cggcgggatc gtgaagatgt
     4141 cccggtccag aatcacgaca tcggccagtt tccccggtgt cagggtgcct tcgacatggt
     4201 cctggtactc ggcgtaggcg gagcccaccg tatagggggg agcggttttg ccagaaaaat
     4261 cgcgtataca gtgcctggct gcgggtactg atcacacaga gagttcaacc agatgggcag
     4321 gacggcgaga tgcattcaag tgccttggct gcgggtactg atcacacaga gagttcaagt
     4381 gccttggcta cgggtactga tcacacagag agttccagca gtcggggcag acagcggcga
     4441 tgcgttcaag tgcggtggcc ggggagatcg ggtgtctctt accctggccg cattttccgg
     4501 ttctcaggca agggggcagg gttcgcgtag gggaagttgc ggtgggggca ggcggatgaa
     4561 tcggtggttg ttcgggttgc cggagggtgc gtcgagggtg agagactggg cgggtgagcg
     4621 ttggggaccc cgttgtgctg gacctgcggc tcgggcttgt ggcttgggat gacgatcaat
     4681 cgccttcgat gagcggtttg gggcgcaggc cggtcgagct gaggctcccc atggaggcgc
     4741 ggtgtggatt tgcgggagtg cgggccaggg cgcgggcgcg ggtgatggac gtccccatcc
     4801 gactcgagtc cgccgtcgat gagggcgcga gcgcttcggg cgcgggctag ggcacgggcg
     4861 gaccgaggcg gttttgttac cacctgacgg agaccggtaa cactttcggt aacaaaatag
     4921 aaaggccaaa aggaagggcc tctatctatc tatctatctt ttctaagtat ttaataataa
     4981 agaagaagaa gaagaagaag gcccgttgac cggagggccg ttctgcgcgt ggtttaggcg
     5041 ttgttaccga tgttaccgat gttaccagag aaagactccc atataggcgg gctttttcgt
     5101 tttaggc
//
LOCUS       DAOQRO010000269         5050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17330_length_5050_cov_4.338338, whole genome shotgun sequence.
ACCESSION   DAOQRO010000269 DAOQRO010000000
VERSION     DAOQRO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17330_length_5050_cov_4.338338"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /gene="lhgO"
                     /locus_tag="PKK20_10065"
     CDS             <1..1194
                     /gene="lhgO"
                     /locus_tag="PKK20_10065"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005369004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNV00274.1"
                     /translation="PSMRSILIVGGGLVGLATAHRLLDHNHRRTVTVLEKESWVGQHQ
                     STHNSGVLHAGLSYLPGSVKARLAVEGIRQMVAFCRAEEIPHEICGKLVVAVELEELP
                     RLDELLRRGAANGLGGLQRLDAAELRRIEPHANGIAAARVPEEGIVDYRAVCDRLAMR
                     IQERGGRIATNARVLRFESRGRGWGALTTAGEYSGDFLINCAGLHCDRVAGLAGERRE
                     VRIIPFRGEYHKLKAERRHLVRHLIYPVPDPKFPFLGVHFTRLIHGGIEAGPNAVLAC
                     AREGYRLTTFDVADILDALSFPGLWRFLRQYPAMAWTELAQSVSRRRFCRSLQRLVPE
                     IRVEDLEPGEAGVRAQAMTPGGSLVQDFHIVARSNALHVLNAPSPAATACLAIGEHIA
                     DQLPA"
     gene            complement(1454..>5050)
                     /locus_tag="PKK20_10070"
     CDS             complement(1454..>5050)
                     /locus_tag="PKK20_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNV00275.1"
                     /translation="NPSFETDTFTVWPGYISDNSPITGWTANAEYRAGLNPATGSPFA
                     DNGAIPDGNNVAFLQSDDGINGTTLETTITGLTAGTTYKVMFRANARSAETPNLMVMI
                     DYVDVLYVTVYPVTETRPYAYIAFEFTASANQHLLTLLNDAAGDHTAVFDNFTIAPTS
                     GRWVVAPWSTDEDSGVDSNYVYTHAYNFGTADNTTINGIIFNGAAGPNPTVTGKFSTT
                     FLGNVFNGDNDANLTGGSQLLGRDFVYSGASVTAGSHQSIKIDGLTEGVEYVATIYSV
                     GFDNPGPAIRWATFSVGDDRLTVNQDQFGNNAGIRLSYRYTADASGSVTLNYAPLNPA
                     NVSFHTYGFSNREAVSRNVAPEITAQPRNVVVAANQPATFMVTATGIPAPTYQWRFKG
                     SDIAGATETSYTISAVTAANVGAYDVVVKNSMGTVTSSAATLTVGIALANPSFEEDIF
                     STWPGYVSDNWDITGWTALGGHGLNPVQDGRSPFADNGMIPHGAQVAFMQQNGALSQT
                     VTGLTTGADYYLHYYENARTGTTVPSMEVQLGSVTLVSAHSVPPVRGGVYYEIYGDAF
                     EAAASELLLSFIKGAPAGGDCTALIDNVAIVEIPSGTAPFVTRNPVSTTVSVGGTASF
                     SVQGIGSLPLVYQWFKNGEEIAGGTGATLTLSNVQKADEAAYSARVSNSAGPVTSAAA
                     NLTVYEPIPDLYSTGLDDARAPLADGAVDPHYTLIENPDVQSTDAIVQDTSVFPISTG
                     TWLPASSTSKWIGPKFNTVDSAVGIFVYRTTFDITGRDPSTVIIEGRWSTDNVGNEIR
                     VNGVATANAPNTGFNLWTPFTIRGTDVDLVAGVNTIDFVVENVQDVGYTGLRVEIIRS
                     NVTIPPGVAPEILIHPLSQTVPEGSTVTLVGNAQGSAPITFQWSKDGTPIPGATGPTL
                     TLADATYEQSGSYTFTAMNASGAATSNPAFVCVCLRPIPGIYSTGVDANGALLEDGAV
                     DPHYTMTASADWQYPGPDALVITNAWPIQAGVWLLNGPDSKWIAASADQREDINPGIS
                     GNAGGEYTFRTTFDLTGYDISKVLIKGQWAVDNTGVDILVNEESTGLVNTAGFGGWTP
                     FTLSEGLVAGPNTVDFVVYNTPNTTYDLNPLGLRVNLKGYLVLDPPAGRPTLTIGRDG
                     ANITVSWTPATATQELQSAPAVTGPWTKITGATSPYATTASGSASFFRVVNP"
BASE COUNT          827 a         1421 c         1781 g         1021 t
ORIGIN      
        1 ccctctatgc gttcgatact gattgttggc ggcggcctgg tcggcctggc gacggcccat
       61 cgtcttctcg atcacaatca tcgacgaacc gtaacggtcc tcgagaagga gtcgtgggtc
      121 gggcagcacc agagcacgca caacagcggt gtgctgcacg ccgggctttc atacttgccg
      181 ggatcggtca aggcgcgtct ggccgtcgag ggcattcgtc agatggtggc cttctgccgc
      241 gccgaagaga ttccgcacga gatctgcggc aagctcgtcg ttgccgtcga actggaggaa
      301 ctgccgcgtc tggacgaact cctgcggcgc ggcgcggcca acggcctcgg ggggctgcag
      361 agactggatg ccgccgaatt gcgccggatc gagccgcacg cgaacggcat cgccgcggcc
      421 cgcgtgcctg aggaggggat tgtggactac cgggccgttt gcgatcgact tgccatgcga
      481 attcaggagc gcggcgggcg gattgccacc aatgcgcggg tgctgcgttt cgagtcgcgg
      541 gggcggggtt ggggagcgct cacgaccgcg ggcgagtaca gcggggactt tctgatcaat
      601 tgcgcgggtc ttcactgcga tcgtgttgcc gggttggccg gcgagcgacg cgaggtgcgg
      661 attattccgt tccggggcga gtatcacaaa ctgaaggcgg agcgccggca cctggtgcgg
      721 catctcattt atcccgtgcc agatccgaag ttcccgtttc tgggggtgca ctttacgcgg
      781 ctcattcatg gggggatcga ggcgggtcca aacgcggtgt tggcctgcgc gcgcgagggt
      841 tatcggctga ccacgttcga cgtggcggac atcctcgacg cgttgagttt tccgggcctg
      901 tggcgattcc tgcgccagta tccggcaatg gcatggacgg agctggcgca atcggtgagc
      961 aggaggcggt tttgtcgatc gttgcagagg ctggtgccgg agattcgagt cgaggatttg
     1021 gagcctggtg aggcgggtgt gcgggcgcag gcgatgacgc ctggtgggag tctggttcag
     1081 gattttcaca tagtggcccg gtccaacgcc ctgcatgtgc tcaacgcccc gagccctgcg
     1141 gccacggcgt gtctggccat cggcgaacac atcgcggatc aacttcctgc gtagaatcct
     1201 tgacatgttt ctggaagggg caagccgcga aaacctggct tgcgccaatc atcgtaatcc
     1261 gccagggcct cttccgcgac cgcgggatca gccctccgag aagggtcggc tttgtgagaa
     1321 gaagagaaag aacccgggga ttgctccccg ggttcttgcg attcatgtgt gccgcgaggt
     1381 gactggcatg tccaggaccc cgcctggcca acctggcgcc ggctcgaagg caggcgccag
     1441 gcggtcgcgg accctacggg ttgacgaccc ggaagaagct ggccgagccg gatgccgtgg
     1501 ttgcgtaggg actggtcgcg cccgtgatct tggtccaagg cccggtcacg gccggtgcgg
     1561 actggagttc ctgggtggcg gtggccggtg tccaggagac cgtgatgttg gctccgtcac
     1621 gcccgatcgt cagggtcggc ctgccggcgg gcggatcgag gaccaggtaa cctttgaggt
     1681 tgacgcgcag gcccagggga ttgaggtcgt aggtggtgtt cggcgtattg tagacgacga
     1741 agtccaccgt gttcggaccg gcgacgagcc cctctgaaag agtgaagggc gtccagccac
     1801 cgaaaccggc ggtgttcacc agtccggtgc tttcctcgtt gacgagaatg tccacaccgg
     1861 tgttgtcgac agcccattgt cccttgatga ggaccttgct gatatcgtaa ccggtcaggt
     1921 cgaacgtcgt gcggaaggtg tactctccgc cggcgttgcc gctgatcccg ggattgatgt
     1981 cttcccgctg atcagcgctc gccgcaatcc acttcgaatc cggaccgttg agcagccaga
     2041 caccggcctg gatcggccat gcattggtga tgaccaacgc atcgggtccc gggtactgcc
     2101 agtcggcact cgcggtcatc gtgtaatgcg gatcgactgc gccgtcttcg agcaaggcgc
     2161 cgttggcgtc gacgcccgtg ctatagatgc ccgggatcgg gcgcaggcaa acgcagacga
     2221 acgccggatt gctggtggcg gcgccggagg cgttcattgc ggtgaacgta taagaaccgg
     2281 actgctcgta ggtcgcgtcc gcgagtgtca gggtgggccc tgttgcgccc ggaatgggcg
     2341 tgccgtcctt gctccattgg aaggtgatcg gagcgctgcc ctgggcgttg ccgaccagag
     2401 tcacggtcga cccttcaggc acagtctggc tgagcggatg gatgagaatt tccggggcga
     2461 ctccgggcgg gatggtgacg ttgctacgga tgatctcgac gcgcaggccg gtgtagccga
     2521 cgtcctgcac gttctcgacc acgaagtcga tggtgttcac gcccgcgaca aggtccacgt
     2581 ccgtgcccct gatcgtgaat ggcgtccaga ggttgaagcc ggtattcggg gcattcgcgg
     2641 tggcgacgcc gtttacgcgg atttcattgc cgacgttgtc ggttgaccat cggccctcga
     2701 tgatgactgt gcttgggtcg cgaccggtga tgtcaaacgt ggtccgatag acgaaaatcc
     2761 ccaccgcgct gtccacggtg ttgaatttcg gtccgatcca cttcgaggtc gagctggcag
     2821 gcaaccacgt gccggtcgag atggggaaga ccgatgtgtc ctggacgatg gcatcggtgg
     2881 actgcacatc cggattctcg atcagtgtgt agtgcggatc gaccgcgccg tccgcgagag
     2941 gtgcgcgcgc gtcgtcgagc cccgtgctgt acaggtcggg gatcggctcg tagacggtga
     3001 ggttcgcggc cgcgcttgtg accgggccgg cgctgttgct gacacgggct gaataggccg
     3061 cctcgtcggc cttctgcacg ttgctcaatg tgagcgtggc gccggtgcct ccggcgattt
     3121 cttcaccgtt tttgaaccat tgatagacca agggcaggct gccgatgcct tggacggaga
     3181 aactggccgt gccacccacg gacacggtcg tggacacggg gttgcgcgtt acgaacggcg
     3241 ccgtgcccga cggaatctca acgatggcga cattgtcgat cagcgcggtg cagtcgccgc
     3301 cggcgggagc gcctttgatg aagctgagca gaagctcgga ggctgcggcc tcgaaggcgt
     3361 cgccgtagat ttcgtaatag acgccgccgc ggacgggcgg gaccgagtga gcggacacca
     3421 gggtgacgct gccgagttgc acttccatgg acggcacggt tgttcctgtg cgggcgtttt
     3481 cgtagtagtg cagatagtaa tcggcgccgg tggtcagacc ggtgacggtc tgagacaacg
     3541 ccccgttttg ctgcatgaag gcgacctgag ccccatgcgg gatcatgcca ttgtcggcga
     3601 atgggctcct tccatcctgg acggggttga gtccgtgtcc gcccagggct gtccagccgg
     3661 tgatgtccca gttgtcgctc acatagcccg gccaggtcga gaagatgtcc tcctcgaagc
     3721 tcgggttggc gagggcgatg ccgacggtca aggtggcggc gctgctcgtc acggtgccca
     3781 tcgagttctt caccaccacg tcgtaggcgc ccacgttggc ggctgtgacc gcagagatgg
     3841 tgtaactggt ttcggtcgcc ccagcgatat cgcttccctt gaaccgccat tggtaggtcg
     3901 gggcgggaat gccggtggcc gtgaccatga acgtcgcagg ctgattggcc gcgacgacca
     3961 cgttcctcgg ctgggcggtg atttccggcg ccacattgcg gctgaccgct tcgcggttgc
     4021 tgaagccgta cgtatggaag ctgacgttgg cggggttgag cggggcgtaa ttgagagtga
     4081 cgcttccact ggcatccgct gtgtagcgat acgagagtcg gatgcctgcg ttattgccga
     4141 actggtcctg gttcaccgta aggcggtcgt cgcctacgct gaatgtcgcc catcggattg
     4201 ccggaccggg attgtcaaag ccgaccgaat agatcgtggc gacgtattcg acgccttcgg
     4261 tgagtccgtc gatcttgatg ctctggtgcg agccggcggt gaccgacgcg cccgaataga
     4321 cgaaatcgcg tccgagcaac tggctgccgc ccgtcaggtt cgcgtcgttg tcgccgttga
     4381 acacattgcc gaggaacgtt gtgctgaact tgccggtcac agtcggattg ggtccggcgg
     4441 cgccgttgaa gatgatgccg ttgatcgtgg tgttgtcagc ggtgccgaag ttgtaggcgt
     4501 gcgtatagac gtagttcgaa tcgacgcccg agtcttcgtc cgtggaccag ggagcgacga
     4561 cccagcgacc gctcgtgggg gcgatggtga agttgtcgaa aacggccgtg tgatcgccgg
     4621 ctgcgtcgtt gagaagagtc agcagatgct ggttggcgga ggccgtgaac tcgaaggcga
     4681 tgtaggcata gggcctggtt tcggtgacgg ggtagacggt gacgtaaagg acgtccacgt
     4741 agtcgatcat caccatcagg ttcggcgtct cggctgaccg cgcgttggcg cggaacatga
     4801 ccttgtacgt tgttccggcg gtcaggccgg tgattgtggt ttcgagggtc gtgccgttga
     4861 tcccgtcgtc cgactggagg aaggcgacgt tgttgccgtc cgggatagcg ccgttgtcgg
     4921 cgaatgggct cccggtcgca gggttcaaac cggcgcgata ctcggcgttg gcggtccagc
     4981 cggtgatcgg gctgttgtca ctgatgtacc ctggccagac ggtgaacgtg tcggtttcga
     5041 agctcggatt
//
LOCUS       DAOQRO010000270         5037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17398_length_5037_cov_3.452429, whole genome shotgun sequence.
ACCESSION   DAOQRO010000270 DAOQRO010000000
VERSION     DAOQRO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17398_length_5037_cov_3.452429"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..1258)
                     /locus_tag="PKK20_10075"
     CDS             complement(368..1258)
                     /locus_tag="PKK20_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV00276.1"
                     /translation="MNAATSILPSRSSVSHAGPLSAPSPPTREPRSHRSLAFTLIELL
                     VVIAIIAILAGMLLPALARAKSKANMTACLNNMRQLGIATVMYVQENGKYPGCLFASG
                     GFRYVWPLRLFSQMGTNRAVFSCPSARPDSRWDTNINKSLGAPYMTGSGRDPWGVSET
                     ALFSIGYNDWGAFPAFSDKGLGGDVDNPAYEVSEAAVKHPAEMIMLADSKPGDGSSAK
                     PRYGLFDGNIDPTTPAEWPSNRHNKRTVVMFCDGHAESAIRNLVIDPANDVWHRRWNN
                     DNSMSGSWTVNKAEASKTDP"
     gene            1382..4285
                     /locus_tag="PKK20_10080"
     CDS             1382..4285
                     /locus_tag="PKK20_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV00277.1"
                     /translation="MSMVRRQYPFHLIEPKWQRFWEERQSFRAWNPGETLPSEHPFAR
                     RHGLGGRLSSAEEMPPKYFILDMFPYPSGAGLHVGHPEGYTATDILARYRRARGWHVL
                     HPMGWDAFGLPAEQYAIKTGQHPRITSKRNIATFKRQIQALGFSYDWSREVDTTDPGY
                     FRWTQWIFLQLYNSYFDPEWNKATPIALLEDALSDAPRRESEWRGNPFLSGTHKAWIE
                     MPPEQKYGLRRQFLDRWRLAYVAEAPVWWCEALGTVLANEDVVDGKSEVGGHPVVRRP
                     MRQWMLRITAYAERLLADLDTIDWSPSLKDMQRNWIGRSDGAEIDFVVASSGGEASDG
                     APRIRVFTTRPDTLFGATYMVLSPEHKLMDEITTPAQREAVLEYKARAAAKSDLDRTD
                     LSKEKTGVFTGGHAFNPATGERIPIWIADYVLASYGTGAIMAVPAHDTRDYEFARKFS
                     LPIVQVVEPPAGTDWRGYVDDGVAVNSAAGDLSLNGLPTAQAKARMTEWLEAKGLGVR
                     TVNYKLRDWLFSRQRYWGEPFPIVWRQDASGNWFHEALPESALPVLPPELDDYKPTSE
                     GEPPLARAREWVHLGEGVRRETNTMPQWAGSCWYYLRFLDARNDAVFVSRAAERYWMG
                     TSDGRPRSVGPESDTDASGSSCAAADAAPRPGVDLYVGGTEHAVLHLLYARFWHKVLY
                     DLGHVSTPEPFYRLVNQGLILGEDGQKMSKSRGNVVNPDAILDEYGADAFRLYEMFMG
                     PLQDSKPWSTKGVEGVYRFLGRVWRLFIDERSETEFEQNCAAEPRRAAEHLEAIRLSP
                     KIEEAAPAPAQLKALHACIQKVTEDLDGLRFNTAISAMMVFVNEAMTWPNRPVPVLRD
                     FLILLQPFAPHLAEELASRLPPAGEWGAEGLDLAYQPWPRFDPALLIEETLEIPVQVN
                     GKLRDVVRVPVTATAPEFERAALAAPKVQPFIEGKTVKKVIVVPKKLVNIALG"
     gene            4299..4481
                     /locus_tag="PKK20_10085"
     CDS             4299..4481
                     /locus_tag="PKK20_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00278.1"
                     /translation="MTKQEQTVLWVVLALLLTGLAVKTYRAKSAGRSAPEPAAIQSDS
                     LHNPPDSVGDYADREH"
     gene            4462..4878
                     /locus_tag="PKK20_10090"
     CDS             4462..4878
                     /locus_tag="PKK20_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00279.1"
                     /translation="MRTENIDEILDRVTATDPRYRREAYHFLREALDYTQRAISKSNR
                     GKLRHITGQELLAGIRAYALAQYGPMALTLFHEWGVRSCADFGELVFNLVDQNVLGKT
                     ETDSRADFLDGYDFEEAFLKPFRPRSRPGSRVSPRS"
BASE COUNT          872 a         1469 c         1741 g          955 t
ORIGIN      
        1 ctttcctgtc gtggctggtc gacaacccgc ttgcccggcg ccgggcccag gcgggggggc
       61 gtcccgctga acgccagttc gagttgtccc cttccgtttg ccatgtcgat tgtcgtcgtc
      121 atggccgcag agtacgggat ggggtggggc aaatacggtc ccacccctag aaaaaaaacg
      181 ctgctcgcct gtcggcggcg gcaccgtttg cacttcttgc cgcggccgca cggatcaggt
      241 gtattgcacc cgattctccc cggttcccga cgaagatgcc tttccctcgt tgacggcgcc
      301 cgttgcccgc agtctgaatc gccacagcgt ctggtgcgga ttctacgccg tcagcagatt
      361 gtattcgtca gggatcggtc ttcgaggcct cggccttgtt gacagtccag gaaccgctca
      421 tggaattgtc gttgttccag cggcggtgcc agacgtcgtt ggccggatcg atgaccaggt
      481 tgcggatggc gctttcggcg tgtccgtcgc agaacataac gacggtgcgt ttattgtggc
      541 ggttcgaggg ccattcagcc ggggtggtgg ggtcgatatt gccgtcgaac agtccgtacc
      601 tcggtttggc gctgctgccg tctccgggtt tgctgtcggc gagcatgatc atttcggcgg
      661 ggtgtttgac ggcggcctcg ctgacttcat aagcggggtt gtcgacgtcc cctccgaggc
      721 ccttgtcgct gaacgcgggg aaggcacccc agtcgttgta gccgatcgag aagagggctg
      781 tctcgctcac gccccagggg tcgcgtccgc tcccggtcat gtagggcgcg ccgagggatt
      841 tgttgatgtt cgtgtcccag cggctgtcgg gtctggcgga ggggcaactg aacacggcgc
      901 gattcgtgcc catttggctg aacaggcgca ggggccagac gtagcggaag ccacccgagg
      961 cgaagaggca tccgggatac ttgccgtttt cctggacgta catgacggtg gcgatcccca
     1021 actggcgcat gttgttgagg caggccgtca tgttggcctt gctcttggcc cgagccaagg
     1081 ccggcagcag catgccggcg aggatggcga tgatggcgat gaccacgagc agctcgatga
     1141 gggtgaaggc aaggctccga tggctgcgcg gttcccgggt gggggggctg ggggcggaaa
     1201 gcgggccggc gtgcgaaaca ctggaacggc tcggcaagat ggacgtagcg gcgttcatga
     1261 tggtctcttc tgcgagtatt tgcttggctc agtgtacggt gttggacggg gctttgccag
     1321 tgttttaagc ggcaactcca cgattgccct cgaggcaggc tttgcgcaat atccgccgct
     1381 tatgtcgatg gtccgcagac agtatccgtt tcatctcatc gagcctaaat ggcagcggtt
     1441 ttgggaagag cgacagagct ttcgcgcgtg gaatccgggc gagaccctgc cgtccgaaca
     1501 tcctttcgcc cgacggcatg gactcggggg gcgcctgtcg agcgcggagg agatgccgcc
     1561 caagtacttc attctggaca tgtttccgta tccctcgggt gcgggtctgc acgtcggcca
     1621 tccggaaggg tacacggcca ctgacattct cgcgcggtac cggcgggcgc ggggttggca
     1681 cgtgctgcat ccgatggggt gggacgcctt cggcctgccg gccgagcagt atgcgattaa
     1741 gaccgggcag cacccgcgaa tcacatccaa gcggaacatc gccaccttca agcgccagat
     1801 ccaggcgctc ggcttcagtt acgactggtc gcgggaggtg gacacgaccg acccgggcta
     1861 ctttcggtgg acgcagtgga ttttcctgca gctctacaac agctacttcg atcccgagtg
     1921 gaacaaggcg actccgatcg cgctcctcga ggacgcgctg tcggatgcgc cgcggcggga
     1981 atcggagtgg cgtggcaacc ccttcctgag cggcacgcac aaggcgtgga tcgagatgcc
     2041 gcccgagcag aaatacgggc tgaggcgcca gttcctcgat cgctggcgtc tggcctacgt
     2101 ggctgaggcc ccggtgtggt ggtgcgaggc gctggggacc gtgttggcga acgaggatgt
     2161 cgtggacggc aagagcgaag tgggcggcca tccggtggtg cgccggccga tgcgccagtg
     2221 gatgcttcgg atcacggcgt atgccgagag gttgttggcc gatctggaca ccatcgactg
     2281 gagcccgtcg ctcaaagaca tgcagcgaaa ctggatcggg cggagcgacg gcgccgaaat
     2341 cgacttcgtc gttgcctcgt cgggcggcga ggcatcggac ggcgcccctc gaatccgcgt
     2401 gttcacgaca cgtccggaca cgttgtttgg ggcgacgtac atggtgttgt cacccgagca
     2461 caagctgatg gacgagatca cgaccccggc acagcgcgag gcggtgctcg agtacaaggc
     2521 ccgcgctgcc gcgaagagcg atctggatcg caccgacttg tccaaggaaa agaccggagt
     2581 gtttacgggc gggcatgcct tcaacccggc gaccggtgag cgcatcccga tctggattgc
     2641 cgactacgtg ctggccagct acgggacagg cgccatcatg gccgtgccgg cgcacgacac
     2701 ccgcgattac gagttcgcgc ggaagttcag tttgccgatt gtacaggtgg tggagccgcc
     2761 cgcggggacg gactggcggg ggtacgtgga cgacggcgtg gccgtgaact cggcggcggg
     2821 ggatttgagc ttgaatgggc ttccgacggc ccaggccaag gcgcggatga cggagtggct
     2881 tgaagcgaag gggctcggcg tccggacggt gaactacaag ctgcgcgact ggcttttcag
     2941 ccgccagcgc tactggggcg agcccttccc gatcgtctgg aggcaggacg cttccggcaa
     3001 ctggtttcac gaggctttgc ccgaatcggc gctgcctgtg ctcccccccg agttggacga
     3061 ctacaagccg acttccgagg gcgagccccc gttggcccgg gcccgcgagt gggttcacct
     3121 gggagagggc gttcggcgcg agaccaacac gatgccgcag tgggcgggca gttgttggta
     3181 ttacctgcgc ttcctggacg cccgcaacga cgccgtgttc gtcagccggg ccgcggagcg
     3241 gtactggatg ggaacaagcg acggcaggcc gcggagcgtg ggtcccgaat ccgacacgga
     3301 cgcttccggg tcgtcgtgcg ccgctgccga cgccgcgccg aggccgggcg tggacctgta
     3361 cgtgggcggg acggagcacg cggttttgca tctgctttac gcccgttttt ggcacaaggt
     3421 gctctatgat ctcgggcatg tgtcgacgcc cgaaccgttc tatcgtctgg tgaaccaggg
     3481 gctcatcctg ggcgaagacg gccagaaaat gagcaagagc cgggggaacg tcgtcaatcc
     3541 ggacgcgatc ctggacgagt atggggccga tgcgttccgt ttgtacgaga tgtttatggg
     3601 gcccctgcag gacagcaaac cctggagcac caagggggtt gaaggcgtct accgcttcct
     3661 ggggcgcgtc tggcgcctgt tcattgacga gcgcagcgag accgagttcg aacagaattg
     3721 cgcggccgag cccagacgcg ccgcggagca cctcgaagcg atccgtctga gcccgaagat
     3781 cgaagaggcg gcgcccgcgc cggcccaact gaaggcgctg catgcgtgca ttcagaaggt
     3841 gactgaggat ctcgatggac tgcggttcaa caccgccatc tcggccatga tggtgttcgt
     3901 gaacgaggcg atgacatggc ccaacaggcc ggtcccggtg ttgcgcgatt tcctgatcct
     3961 gctgcagcct tttgcgccgc atttggccga ggaactcgcc tccagacttc cgccggcggg
     4021 cgagtggggg gcggagggtt tagacttggc ctatcagccc tggcctcggt tcgatccggc
     4081 gctgttgatc gaggagacgc tcgagatccc cgtgcaagtg aacgggaaac tccgcgacgt
     4141 ggtgcgagtg cccgtgacgg cgacagctcc ggagttcgag cgggcagccc tggcggctcc
     4201 caaggtgcag ccctttatcg agggcaagac ggtcaagaaa gtgatcgtcg tgccgaagaa
     4261 gctggttaat attgcgctgg gttgagactg tgattcattt gaccaaacaa gagcagaccg
     4321 tgctgtgggt ggtgctggcg ctgttgctga ccggcctggc cgtgaagacg tatcgcgcaa
     4381 agtccgcggg tcgatccgcg ccggagccgg ccgcgattca gtccgattcc ctccacaacc
     4441 cacctgacag cgttggagac tatgcggaca gagaacattg acgagattct ggatcgggtg
     4501 accgcgacgg acccgcgcta tcggcgggag gcatatcact ttctgcgcga ggcgttggac
     4561 tatacccaac gggccatcag caagtcgaac cggggcaagc tgcggcacat caccgggcag
     4621 gagttgctgg cgggcatccg ggcctacgcc ctggcgcaat acgggccgat ggccctgacc
     4681 ctgtttcacg agtggggcgt gcgctcgtgc gccgatttcg gcgaattggt gttcaacctg
     4741 gtcgaccaga acgtcctcgg gaagaccgag acggacagcc gggcggattt tctggacggc
     4801 tacgattttg aagaggcgtt cctcaagccg ttccgtcctc gctcccgccc gggctctcgc
     4861 gtttcgccgc ggtcctaacc cgcatctttc acactccgtc atgcacatgg cgatcggatt
     4921 tcgcaagatg tctgctaggc gggttcgaag aacaaaaccg caccaggttc gagaggcatg
     4981 aaaatgaaca cactgttctt atggcaggac ggtttcagaa ctgagaggcg gctctgg
//
LOCUS       DAOQRO010000271         4962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17757_length_4962_cov_6.649277, whole genome shotgun sequence.
ACCESSION   DAOQRO010000271 DAOQRO010000000
VERSION     DAOQRO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17757_length_4962_cov_6.649277"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..3044
                     /gene="ileS"
                     /locus_tag="PKK20_10095"
     CDS             210..3044
                     /gene="ileS"
                     /locus_tag="PKK20_10095"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNV00280.1"
                     /translation="MDYKDTLNLPRTDFPMKADLAAREPNRLAAWEAARLYHQIQSAR
                     ATAPKFVLHDGPPFANGEVHVGTAWNKILKDITIKYKTLRGFQAPYVPGWDCHGLPIE
                     FKVGQEMRKSGVAATDPAVIRKACDAYARKFIDIQRAQFKRLGVLADWEKPYLTLDKE
                     YEAAELRLFADLIERGFIYRGKKPVYWSIPCRTALAEAEVEYQDHVSQSIYVKLPIVG
                     HPGLNAVIWTTTPWTLPANLAVAYNSTFSYSLVLAGTESYIVSTLRLSAVAQQCGWDG
                     YQIIQSLTGEHLAQLEYQHPFCQRTGKFFAGDEFVDNATGTGFVHVAPGHGLEDYHLG
                     MRVGLPIYSPVDDDGCFTHTPDLPDDQQMPTDMLGKSILEKHGKSDANETVLHALRVR
                     KALLHQENYHHSYPHCWRSKTPIIFRAMNQWFLRVDHAPPDAPAPFRQQALEAIGQVN
                     WVPAWGRTRIEAAVKGRPDWCISRQRAWGVPIPAFSDPQGNPILDARIVRRVADLVAR
                     HGSQIWFEQTAAELWPQVKPADWTSPDPVAKMTDTLDVWIDSGSSARSVLMQRPELQQ
                     PDSARSIHPWQADLYLEGSDQHRGWFQSSLLLSLAANGAAPFRTVLTHGFMVDADREK
                     ISKSKQSQGGYEKPQNAEAYVKRWGADILRLWVASQDFSSDVVVSEDRLAKVAETYRM
                     LRNTLRYQLSNLYDFDPAQHRVPDDQLTGLDRWILDAFAKLEGDVLKAYDSYQFHVVY
                     QRVSQFAAVDLSAVYHDVVKDRLYTDPPDSTRRRATQTALWRLVTGLCLMLSPILVFT
                     ADEVWEHVPGHPTPSVHLAAWSATRIDRTDAEQNLWKRLFALREAALPELEKARQAKL
                     IGKALEGRLAIRAPAAALIEVRSHCDALRELLNVSQIDLVTDDGATTPVLSVARAEGQ
                     KCERCWHWKTDVGQHGAHPTLCGRCVQAVS"
     gene            3343..3861
                     /locus_tag="PKK20_10100"
     CDS             3343..3861
                     /locus_tag="PKK20_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00281.1"
                     /translation="MTKHRHDRAHRLGKRKRRFPMGLVGLVLGTVLTAAIFIVAPRFS
                     GLPKSTPPRSTDAPAATTPASDPKAKANPQRLIGRWLRPDGGYILDIQGVKGDRSLEV
                     AYLNPNPIHVSRAETEEDGKTLRVFVELRDVNYPGCTYKLTYDPDKDQLTGIYFQAAL
                     NETFDVAFARMK"
     gene            complement(3884..>4962)
                     /locus_tag="PKK20_10105"
     CDS             complement(3884..>4962)
                     /locus_tag="PKK20_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV00282.1"
                     /translation="VAAISALGKIGGEAAFDALRAVAWRFDPALGEGTSISVNLSEDV
                     RRRLDVRYLFDALLMCAERLIDNDRADRAADTFARIYRSTSAPRPQRIAALVGLTRAA
                     PDRAVPLILQLLRGPDPASRAAAANLVGRLPPASAAPIVTLLGTLPTVQQALVIEAVS
                     GCSDRALLFPLLKATMSPDPAVRVTALRALGHQQGNRQIVQLLVAAASGGGAESAVAR
                     ESLSRIEGPEVDADLMAQLDQTSVAMRAEAARGLGVRGVTNAVPRLVKALGDANGPIR
                     FAAIESLGQLGGEQQLQDLLDYLGRADVAAEQEVAARAVLAIARRIASVSPGPARAAA
                     EKVADSTTAGPIQSEAKAILDSIK"
BASE COUNT          945 a         1815 c         1392 g          810 t
ORIGIN      
        1 caggttcttt attgatcgcg ctcaaggcgg accttcaccc cgaggcttct tggcccgccc
       61 tgcaggagcg ctgccagaac aatccccaaa tccctttcca gatactcgtc aagcaccgct
      121 ccaaatgaaa agaccgtggt gtctgaggcg cgcctcgtga ggtgacgctt gtcaacgcat
      181 ggcagggctt ggtacgttcc cgcgcttcga tggattacaa agacacactc aatctgcccc
      241 gcaccgactt ccccatgaag gcggatttgg cagcacgcga acccaaccga ttggccgctt
      301 gggaggcggc ccgtctctat caccagatcc aaagcgcccg cgccacggcc cccaagttcg
      361 ttctgcacga tggcccgccc ttcgcgaacg gcgaggtcca cgtcggaacc gcctggaaca
      421 agatcctcaa ggacatcacg atcaagtaca agaccctccg cggcttccag gcgccgtatg
      481 tgcccggctg ggattgccac ggtctgccca tcgaattcaa agtcggccag gagatgcgca
      541 agtcgggggt ggccgccacc gaccccgccg tgatccgcaa agcctgcgac gcctacgcgc
      601 ggaagtttat cgacatccag agagcccagt tcaagcgcct cggcgtcctg gccgactggg
      661 agaaaccgta ccttacgttg gacaaggagt acgaggctgc cgaactgcga ttgttcgccg
      721 acctcatcga gcggggcttc atctatcggg gcaagaaacc cgtctattgg agcatcccct
      781 gccggaccgc cctggccgag gccgaggtcg agtatcagga ccacgtcagc cagagcatct
      841 atgtgaagct ccccatcgtc ggacacccgg gactcaacgc ggtgatctgg acaaccacgc
      901 cttggacgct cccggccaat ctggccgtcg cctacaactc gacgttcagt tactcgctcg
      961 tgctggccgg caccgagagt tacatcgtct ccacactccg cctgtccgcc gtcgcccagc
     1021 agtgcggctg ggatggctac cagatcatcc agagcctcac cggcgaacat ctggcccaac
     1081 tcgaatacca gcacccgttc tgccagcgca ccggcaagtt cttcgccggc gacgaattcg
     1141 tcgataatgc caccggcacc ggcttcgttc atgtcgcccc cggccacggc ctcgaagact
     1201 atcacctggg aatgcgcgtc ggcttgccca tctactcgcc cgtcgatgac gacggctgct
     1261 tcacccacac ccccgacctg ccggacgacc aacagatgcc gaccgatatg cttggcaagt
     1321 cgatcctcga aaaacacgga aaaagcgacg cgaacgaaac ggtgctgcat gcgctgcggg
     1381 tgcgcaaggc gctgctccac caggagaact accaccacag ctatccccac tgctggcgca
     1441 gcaaaacacc catcatcttc cgcgccatga accaatggtt cctccgggtc gaccatgcgc
     1501 cgcccgacgc gcccgccccc ttccgacaac aagccctcga ggcgatcggc caggtcaatt
     1561 gggtgcccgc ctggggccgc acgcgaatcg aagccgcggt caaaggccgg cccgattggt
     1621 gcatctcccg ccagcgcgct tggggcgtcc ccatccccgc cttctccgat ccccaaggca
     1681 atcccatcct cgatgcccgc atcgtccgcc gcgtcgccga tctcgtggcc cgccacggct
     1741 cgcagatttg gttcgagcaa accgccgccg aactctggcc ccaggtcaag cccgccgatt
     1801 ggaccagccc ggatcccgtg gccaagatga ccgacacact cgatgtctgg atcgactccg
     1861 gctcgtccgc acgctcggtc ctcatgcagc gtcccgaact ccagcagccc gactccgctc
     1921 ggtccatcca cccctggcag gccgacctct acctcgaagg gtcggatcaa caccgcggct
     1981 ggttccagtc ctcgctcctt ctctcgctcg ccgcaaacgg cgccgcgccc ttccgcacgg
     2041 tgctcacgca cggattcatg gtggacgcgg atcgcgagaa aatctcgaag agcaaacaga
     2101 gccagggagg ctacgaaaaa cctcaaaacg ccgaggccta tgtgaagaga tggggcgccg
     2161 atatcctccg gctctgggtc gcctcccagg acttcagcag cgatgtcgtg gtcagcgagg
     2221 atcggctcgc caaagtcgcc gagacatacc gcatgctgcg caacacgctc cgctaccaac
     2281 tctcgaatct ctacgacttc gaccccgccc aacaccgcgt ccctgacgac caactgaccg
     2341 gcctcgaccg ctggattctc gacgcgttcg ccaagctcga gggggacgtc ctcaaggcct
     2401 acgacagcta tcagttccac gtcgtctatc aacgggtcag ccagttcgcc gcagtcgatc
     2461 tgtccgcagt ctaccatgac gtcgtcaaag accggcttta caccgatccg cccgattcca
     2521 cccgacggcg agccactcag accgctctct ggcgtctggt gactggcctg tgcctgatgc
     2581 tctcgcccat ccttgtcttc accgccgatg aagtctggga acacgtccct ggtcacccca
     2641 ccccctccgt ccacctcgca gcctggtccg cgacacggat cgaccgcacc gacgcggaac
     2701 agaacctctg gaagcggctc ttcgcgctcc gcgaggccgc cttgcccgaa cttgaaaagg
     2761 cccgccaggc caaactcatc ggcaaagccc tcgaaggacg cctcgcgatc cgtgccccgg
     2821 cagccgcgct gatcgaggtc aggtcgcact gcgacgcgct ccgcgagttg ctgaacgtgt
     2881 cgcaaatcga tctggtgacc gacgacggcg ccacaacccc cgtcttgagc gtcgctcgcg
     2941 ccgagggtca gaagtgcgaa cgctgctggc actggaaaac ggatgtcggc cagcacggcg
     3001 ctcatccgac cctctgcgga cgctgcgtgc aggccgtaag ttgatccgcg cgccaagccc
     3061 gggctcgctg ttccgaacag ggttcccggc aacgaacgga actcgaatgg ctcctgattt
     3121 tggggtcatg caggccttgc accagcctgc gatactgggc tccggcgaac gtgccgccca
     3181 gacaaccgtc ccgcaattcc acttccgcct ccagtccaac gagcgccgac accagtgcct
     3241 cccttccctc cggtattgcc tctgcagacc ggacgttcct agcgtctttt ctacgtgcca
     3301 atccaggcgg ttggtggttc tctccttttt gaacacaccc ttatgaccaa acatcgacac
     3361 gatcgcgcgc atcggcttgg caaacgcaag cgccgcttcc cgatgggact cgtggggctg
     3421 gtgctcggca cggttctcac cgccgcaatc ttcatcgtcg cccccagatt cagcggcctc
     3481 cccaaatcca ccccgccccg ctcgaccgat gcgccggccg ccaccacccc ggcctcggac
     3541 ccgaaggcca aggccaatcc ccagcggctg atcggacgat ggctccgccc ggacggcgga
     3601 tacatcctgg acatccaagg cgtcaaaggg gatcgctcac tcgaagtggc ttacctgaac
     3661 ccaaacccca tccatgtctc ccgcgccgaa accgaagagg atgggaaaac actccgtgtg
     3721 tttgtcgaat tgcgcgacgt caattacccg ggctgcacgt acaaactgac ctacgatccc
     3781 gacaaggacc aactcaccgg catctacttc caggccgctc tgaacgagac gttcgacgtc
     3841 gccttcgcac ggatgaaatg aagcgccaac gaggcgcgcc acatcacttg atcgagtcca
     3901 ggatcgcctt ggcttcggac tgaatagggc cggccgttgt cgagtcggca accttctccg
     3961 ccgctgcccg cgccggaccg ggcgacacgg aggcgatgcg gcgcgcgatc gccagcactg
     4021 ctcgggccgc tacctcctgt tcagccgcca cgtccgcccg accgaggtaa tccagcaggt
     4081 cctgaagctg ctgctcccca ccgagctgcc ccaggctctc gatggccgca aagcgaatgg
     4141 gaccgttggc gtcacccagg gctttcacaa ggcgcggaac ggcgttcgtg acgcctcgga
     4201 ccccaagacc acgcgcggcc tcggcccgca tcgccacgct ggtctgatca agctgagcca
     4261 tgagatccgc gtccacctcc ggcccctcga tccggctcag gctctcccga gccacggcgc
     4321 tctccgcacc cccgccgctc gccgccgcaa ccaggagttg aacgatctgg cggttgcctt
     4381 gctggtgccc caacgcgcgc agcgcggtaa cccggaccgc ggggtcggga ctcatggtcg
     4441 ccttcaacag agggaagagc aacgcgcggt cggaacaccc gctcaccgct tcgatgacca
     4501 gcgcttgctg gacggtcggc agggtgccga gcagcgtgac aatcggcgca gccgaggcag
     4561 gaggcaaccg ccccaccagg ttcgccgctg ccgcccgcga ggctggatcc ggaccgcgca
     4621 ggagttgcag gatcagcggc accgcgcggt caggagcggc gcgcgtcaac ccgacgagcg
     4681 cggcgatgcg ttgcggacgg ggcgccgacg tcgagcggta gattcgcgca aaggtgtcgg
     4741 ccgcccggtc tgcccggtcg ttatcgatca ggcgctccgc gcacatcagc agcgcgtcga
     4801 acagatacct cacatcgaga cgccgtcgca cgtcttcgga cagattgacc gagatcgatg
     4861 tcccctcgcc caacgcggga tcgaagcgcc atgcaaccgc gcgcagcgcg tcgaacgcag
     4921 cctctccccc gatcttgccc agcgcactga tggcggccac cg
//
LOCUS       DAOQRO010000272         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17764_length_4961_cov_4.616184, whole genome shotgun sequence.
ACCESSION   DAOQRO010000272 DAOQRO010000000
VERSION     DAOQRO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17764_length_4961_cov_4.616184"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKK20_10110"
     CDS             complement(<1..311)
                     /locus_tag="PKK20_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HNV00283.1"
                     /translation="MALNEKLEGLRTVLRGYGSCLVAYSGGVDSVFLAYVARQVLGDS
                     LLAAICDSPSLPRRDLDEALALAARLDIPVRVVQTAEFASSDYLSNSRERCYFCKHLV
                     MG"
     gene            436..>4961
                     /locus_tag="PKK20_10115"
     CDS             436..>4961
                     /locus_tag="PKK20_10115"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00284.1"
                     /translation="MASGSLWITLIERVMNIFLDRMNRLDSTAPPSKDRGRTRPAIVR
                     ARKPVSLSGHGFSALARLRATPLLVLAMCLWWNGANALRAGVVFGEDTEFRYFKGLEE
                     ASKPDSTAWRSIGFDDSAWLIGRGPFYYEDSSGFTGNTPLTDMRGRYSCIYLRKTFTV
                     QSPAAVSEMTFTIQADDGCVLWLNGTEIGRINMPDGEPAFNWLSLPAAGEPNVASFTV
                     TDAPSLLKAGANVVAVQAFNSGLSNSTDFLIAVACTSTLDITPPTLAEVAPAPGATVR
                     ALSVIQISFSENVTGVDASDLLINGAPAASLTAYSPRDYAFVFAEPPAGRVTVTWAPN
                     AGIGDLAIPPNLFQGGSWDYIVDPTVPAANIIISEFLADNGNGIRDDFGNRSDWIELL
                     NRGGEAASLLGWSLTDDRGNPAKWRFPDITIGPNSYLLVWASGRALADPLAALHTNFR
                     LSNEGDYLGLADPEGRIVSEFAPVYPPQRKDISFGRDRTSPELTGYFTTPTPGAGNAL
                     TGPGFAPDPVLSIKGGVYAASSLTLSMSAPSGTIRYTLDGSTPSGSSTAYTAPINISA
                     STIVQARVFQAGLLPSSIVVETYNLLGVGVANFSSNLPLLIINTAGRGISQDSRVPAF
                     VTAIDTYRGRAALRAPPTFQGKAQIEIRGQSSAGFPKKAYNLELNDAVGNDLEVPLLG
                     LPKESDWVLYAPYTDKPFLQNFLAFELHEKMGHYAPRRRFVEVFVDTSGGRLDYPRDY
                     AGIYILLEKIKVDANRVDIARLTPQHNAEPEITGGYIVKKDKDSPGDLNFSTSGGGSF
                     GGQALKYHEPKPREITIAQRNWIRNYLNQFERAMYAANWLGATGTNHYSHYIDVDSFV
                     DNHWIVEFAKQIDGYRLSNYMSKDRGGKLRMDPIWDWNLSFGNADYLEGWLTNGWYYT
                     QIDANAHIWLRRLMCGTTSSSGTTGDPDFNQRIADRWSVLRTNIFASSNVLARIDELA
                     ASLNEAQVRDFQKWPRLGAYVWPNPSMYVTPTTYAGIIANLKSWVHGRYHWIDSQFVK
                     APELSRGDCAVSPGFVLTTSGPAGTVYYTLDGSDPRAKGGGIAPGARAYASGIPIQTN
                     SRVVARVRSGNRWSGPAAATFLVETPPLVITELMYAPAAPDPKNGDEPGDLEFVELKN
                     IGTAPLDLRGFEFTHGIQFSFSSGQAVSLAPGQTVVVVKNAAAFARRYPHVQNVAGEY
                     RGSLANEGERVRLVGPMLEEVHDFRYEPDWYPATDGLGFALVVADEHQPRSAWNRREG
                     WRVGTAFLGTPGQLEPDAPDIPAVRVNEALTRAQSPLLRSIELHNPTSAAVDVGGWFL
                     SDDPDTPKYRIPSPTLIPAGGFLVFTERDFNPNPGVPPSFNLGAAGDDVYLFSADATG
                     RLTGYRHGFKFGPALAGVSFGRHVTSDGLEHFVAQTTCTLGNPNGPPRIGPVVISEIM
                     YHPPDVVTNNAAWNNTEDEYIELCNVTTSAVALHDPAAPTNTWRLRNAVQYAFPSSEY
                     LAPGERILVVSFDPS"
BASE COUNT          938 a         1731 c         1424 g          868 t
ORIGIN      
        1 cccatcacaa ggtgcttgca gaaataacag cgctcgcgcg aattggacag gtagtccgaa
       61 ctcgcgaatt cggcggtctg gaccacgcgg accgggatgt ccaaccgggc cgccaacgcc
      121 agcgcctcat cgaggtcgcg cctgggcaga cttggagaat cgcaaatcgc cgcaagcagc
      181 gagtcgccca agacctgccg cgccacgtat gccagaaaga ccgagtcgac tccaccggag
      241 taggcgacaa ggcacgagcc atagccgcgc agcaccgtcc ggagcccttc gagcttttcg
      301 ttcagagcca tggacgccaa agtggcacgc cttcagccag ttgtcgagga cccgacgcgc
      361 tccggcgcca acgcaacgct tgccgtcccg cgcctcagaa tcagcacggc agagaatctg
      421 tcccgcgcgt gacccttggc ttctggctcg ctgtggatta ctctcatcga gcgcgtgatg
      481 aacatcttcc tcgaccgcat gaatcgactg gactcgaccg ctccgccctc gaaggacagg
      541 ggccgcaccc gccctgccat cgtcagagcc cgcaaacctg tgagtctctc cggacacggg
      601 ttttccgcgc tggcccggct tcgggcaacg ccgcttctgg tgctggcgat gtgtttgtgg
      661 tggaatggtg ccaacgcttt gcgagcaggc gtggtctttg gggaggacac cgagttccgt
      721 tatttcaagg ggctcgagga ggcttcgaag cccgactcga cggcctggcg ctcgattggc
      781 tttgatgaca gcgcctggct gattgggcgc ggcccattct actacgaaga ctcgagcggt
      841 ttcaccggca acaccccgct gaccgacatg cggggccggt acagttgcat ctacctgcgc
      901 aagacgttca ccgtccaatc tccggccgcg gtctccgaaa tgacgttcac aatccaggca
      961 gacgatggct gcgtcctgtg gttgaatgga acagagatcg gacggatcaa catgcccgac
     1021 ggcgaaccgg cattcaactg gctgtcgctt ccggcggccg gcgaacccaa tgtggccagc
     1081 ttcaccgtca cggacgcgcc cagcctcttg aaagccggcg ccaacgtcgt cgcggtccag
     1141 gcgttcaact ccggcctcag caacagcacc gacttcctca tcgccgtggc ctgcacaagc
     1201 actctggata taacaccccc gacattggcc gaggttgccc ctgcaccggg cgccacggtt
     1261 cgggcgctct cggtcatcca gatctccttc agcgaaaacg tcactggcgt cgatgcgagc
     1321 gatctcttga tcaacggtgc gcccgccgca tcgctcaccg cctattcccc gcgcgattac
     1381 gccttcgtct tcgcggagcc tccggcgggg cgagtcactg tgacgtgggc cccaaacgcc
     1441 ggaatcggcg atctcgcaat cccgccgaac ctcttccagg gaggaagctg ggactacatc
     1501 gtcgatccga ccgttccggc agcgaacatc atcatttccg aattccttgc cgacaatgga
     1561 aacggaattc gggatgattt cggcaatcga tcggactgga tcgagttgct gaatcgtggc
     1621 ggcgaggcgg caagcttgct cggctggtcg ctgaccgacg accggggcaa tccagccaag
     1681 tggcgtttcc cggacatcac gatcggaccc aacagctacc tcttggtctg ggcgtccggt
     1741 cgggccttgg ccgatccttt ggccgcgttg cacacaaact tccgcctctc gaacgagggt
     1801 gattacctcg gactggcgga ccccgagggc cggatcgtgt ccgagttcgc gccggtctat
     1861 cccccgcagc ggaaggacat ctcgtttgga cgcgaccgga cgagtccgga actcacgggt
     1921 tatttcacca cgccgacgcc gggggcagga aatgcgttga cggggccggg ctttgcgccg
     1981 gacccggtcc tctcgatcaa gggtggcgtc tatgccgcgt ccagcctgac gctgagcatg
     2041 agcgcgccct cgggcacgat tcgctacacg ctcgacggct cgacgccctc gggctcctcg
     2101 acggcgtaca cggcgcccat caacatcagt gcaagcacca tcgtccaggc ccgggtcttc
     2161 caagccggtt tgttgccgag ctcgatcgtc gtcgagactt acaacctgct cggagtcgga
     2221 gtggcgaact tcagctcgaa cctgccgctg ctaatcatca acaccgcggg tcgtggtatc
     2281 tctcaagact cccgcgtgcc ggccttcgtc actgcgatcg acacgtaccg cgggcgggcg
     2341 gcgcttcggg cgccccctac ctttcagggc aaggcccaga ttgagattcg cggtcaatcc
     2401 tcggccggtt ttccgaagaa ggcctacaat ctcgaactca acgatgccgt cggcaacgac
     2461 ctcgaagtgc ccctgctggg cctgcccaag gaatcggatt gggtgcttta cgctccatac
     2521 acggacaaac ccttcctcca gaacttcctc gccttcgaat tacacgagaa gatgggccac
     2581 tacgccccgc gccgacggtt cgtcgaggtc ttcgtcgaca cctcgggcgg tcgactcgac
     2641 tacccgcgcg actacgcggg catctacatt ctgctcgaga agatcaaggt ggacgccaac
     2701 cgggtcgaca tcgcccgcct gactccccag cacaacgccg aacccgagat cactggcggc
     2761 tacatcgtta agaaggacaa agacagccca ggcgacctca acttcagcac gtcgggagga
     2821 gggagcttcg gcggccaggc cctcaagtac catgagccca agccgcgcga gatcactatc
     2881 gcccagagaa actggattcg gaactatctc aatcaattcg aacgtgcgat gtacgcggcc
     2941 aactggctgg gcgccaccgg cacgaaccac tattcgcact acatcgacgt cgattcgttc
     3001 gtggacaacc actggatcgt cgagttcgcc aagcagatcg acggctatcg cctgagcaac
     3061 tacatgagca aggatcgggg cggcaagctc cgcatggacc cgatctggga ctggaacctg
     3121 agcttcggca acgccgatta cctcgaaggc tggctcacca acggctggta ttacacccaa
     3181 atcgacgcca acgcccacat ctggctgcga cgcctgatgt gcggcactac atcctcgtcc
     3241 ggaacaaccg gcgatcccga tttcaaccag aggatcgccg accgctggag cgtgctgcgc
     3301 accaatatct tcgcctcctc gaatgtcctc gcccgcatcg acgaattggc cgcgtcgctc
     3361 aacgaagccc aggtccgcga cttccagaaa tggccgcgcc ttggcgccta cgtctggccc
     3421 aacccgtcca tgtatgtcac ccccaccacg tacgccggca tcatcgccaa cctcaagtcc
     3481 tgggtccacg gccgctacca ctggattgat agccaattcg tcaaagcgcc tgaactgagc
     3541 cgcggcgact gcgccgtctc acccggattc gtcctcacca cctccggccc ggccgggact
     3601 gtctactaca ccctggacgg ctccgacccg cgggccaagg gaggcggcat cgcgcccggg
     3661 gcgcgggcat acgcgtccgg catcccgatc cagaccaaca gccgcgtggt ggcccgcgtg
     3721 cgcagcggca accgatggag cggccccgcg gcggccacct tcctcgtcga gacccccccg
     3781 ctcgtgatca cggaactcat gtacgccccc gccgctcccg atccgaagaa cggcgacgag
     3841 ccgggcgatc tcgaattcgt cgaactgaag aacatcggca ctgcgcccct ggacttgcgc
     3901 ggcttcgagt tcacacatgg catccagttc tcgttctcgt ccgggcaggc cgtctcgctc
     3961 gcccccgggc aaacggtcgt ggtggtgaag aacgccgccg catttgcccg ccgctacccc
     4021 catgttcaaa acgtcgccgg agaataccgg gggtccctgg ccaacgaagg cgaacgggtg
     4081 cgcctggtcg gtcccatgct cgaagaggtg cacgacttcc ggtatgaacc cgattggtat
     4141 cccgccaccg atggactcgg attcgcgctg gtcgtcgcgg acgaacacca gccgcgttcg
     4201 gcctggaacc ggcgtgaagg ctggcgcgtc ggcactgcct tcctcggcac gcctggacag
     4261 ctcgaacccg acgcgcccga catccccgca gtgcgcgtca acgaagcgct cacccgcgct
     4321 caatccccgc tgctgcgatc gatcgaactg cacaatccga ccagcgcggc ggtcgacgtg
     4381 ggcggctggt tcttgtcgga cgacccggat acgcccaagt accgcatccc ctcgcccacc
     4441 ctgattccgg caggcggctt cctggttttc accgagcgcg atttcaatcc caaccctgga
     4501 gtgcccccca gcttcaatct cggcgctgcc ggcgacgatg tgtacctgtt ttccgccgac
     4561 gccactggcc ggctcaccgg ctaccggcac ggcttcaagt tcggcccggc cctcgcaggg
     4621 gtttcgttcg gacgccacgt caccagcgac ggactcgaac actttgtcgc ccaaacgacc
     4681 tgcactctgg gcaatcccaa tggccccccg cgcatcggac cggtcgtcat cagcgagatc
     4741 atgtaccatc cgcccgatgt cgtgaccaac aacgccgcct ggaacaacac cgaggacgag
     4801 tacatcgagt tgtgcaacgt gaccacgtcg gccgtcgccc tccacgatcc cgccgccccc
     4861 accaacacct ggcggttgcg gaacgctgtt caatacgcct tcccgtcaag tgaatacctg
     4921 gcgcccggcg aacgaatcct ggttgtcagt ttcgatccgt c
//
LOCUS       DAOQRO010000273         4943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_17857_length_4943_cov_5.089812, whole genome shotgun sequence.
ACCESSION   DAOQRO010000273 DAOQRO010000000
VERSION     DAOQRO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17857_length_4943_cov_5.089812"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKK20_10120"
     CDS             complement(<1..243)
                     /locus_tag="PKK20_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00285.1"
                     /translation="MWKRRSKRLARFLAIAALVCVAGLAVLKVWLDASYFDGYDPNAP
                     LNLELALVEERQAYTWTKFYYAGARGDRVPAVMATPK"
     gene            451..3516
                     /locus_tag="PKK20_10125"
     CDS             451..3516
                     /locus_tag="PKK20_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNV00286.1"
                     /translation="MKNTVFLIAWAGAVSVLDTACLGQNLLPNPSFERSQERVEGWTL
                     SGGKGRVVRAEGNAAAGQVCIGLEGDGKEPNWWRTADLALQPGGLYCFGFMGRRAAEA
                     HGGTAMSGPSRVNRDFPLDHRWGREEFIFMNPHNNPRDFVRLGQWEVDGRLDFDCVEL
                     VPVFAAHAETAEGIELGEAESVRAGVYRFEPNLNWRGANYHRPLVTNQAGFNSNRWLF
                     WPGAEVVYRFAVSGSPQISASLRAGINYHTGGVLRIDASRDGQAWVAVAEFDGQKRGG
                     RAELPMSLFPADQVLVRLSQSGQEAGFQVDTFEYEAALENPPPDADGETLFIESREAS
                     PEVGIALAGWTCRAADGDFCIAIVATNRAPRAVAFTASATCLGIGQEAGPAVRSMTAS
                     VPPGETGRFDLRLGFPGAGENRIALTITNDAGQGVFRGTSMLRVGLLEDASYGFWLAD
                     EGGVTAWWCESGWKVGRDRVAPVPARMGERRAIEIAAARNEFEAVQLVLRPDRETTLL
                     ETPAVTLRDERGDSSPIEVRFDEVAYVHVTQPTDASCVRGWYPDPLPPLKTPLKLAAH
                     RNQPLWITIRVPESARAGRHRGEIEVRTSAGTLRAGLEVDVYDFALPAETHLRSALGL
                     GTHEINRYHRLSHGVDKEAVFAKYLRNFAEHRISPYSFFEYAPFRVRFEGEGANKRAR
                     VDFAQFDVAARTWLDQHHFSTFQLPLRGMGGGTFHSRHLGELEGFQEGTPEHAALFRD
                     YLGQVERHLRENGWLGKAFTYWFDEPDPKDYAFVVAGQQRIKEAAPGLRRMLTEQPEK
                     ELIGHVDIWCGLTPEWTRERVRARKEAGEEVWWYICTGPKAPYVTEFIDHPGTELRLW
                     PWQSWQYGVSGILVWATVYWSSPLAYPEPNVQNPWADPMSWVSGYGNPVGYRRPWGNG
                     DGRFLYPPRRDPNASTAPNLDAPINSLRWDNLRDGMEDYEYLWLLEREVERVGDREAS
                     SALVREARSLLEVPESVSKDLTHFTTDPRPILAHRDKVARMIERLRLIE"
     gene            complement(3539..>4943)
                     /locus_tag="PKK20_10130"
     CDS             complement(3539..>4943)
                     /locus_tag="PKK20_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNV00287.1"
                     /translation="NLAGVTLGTKVATIGRAAFAGCTSLTAITIPNTVTSLGNEVFAD
                     CVNLAGVTLGTKVATIGRAAFARCTSLTGITIPNTVTSLGNEVFADCVNLASVTLGTK
                     VASIGRYAFCRCTSLTSITIPRSVTSIQAWAFWACTNLANVTIPNTVTTIGDEAFESC
                     TSLTSVTIPSSITTIGVAVFYRCTSLTSVTIPESVTRIGAYAFYGCSSLPSITIPSRV
                     TSIGPYAFCRCASLTAIAIPNRVTSIGRYAFFACTGLADVAIGPSVTSIGPYAFGSCT
                     GLTSVTIPKGVTSIDAWLFSDCAGLTSVTIPDTVTRIADGAFFGCTDLTRITLPPKVT
                     SIGEHAFAGCTGLASVCFLGNAPATSTAFDDCPATVYYLPETTGWEPTFSGRPTAVWV
                     PRIEAADADFGVHANRFGFDITWTPKSIVVVEACSDPANPIWVPVSTNTLATGLSSFN
                     DPKWTDFPARFYRLRSK"
BASE COUNT          831 a         1470 c         1744 g          898 t
ORIGIN      
        1 ctttggggtc gccatcacag ccggaacgcg atcgccgcgt gcgcctgcgt agtagaattt
       61 cgtccacgtg taagcttgtc tttcctcgac cagggccagc tcgaggttca agggtgcgtt
      121 tgggtcgtag ccgtcgaagt aagaagcatc cagccacact ttgagtacgg caagaccggc
      181 cacgcacacc agcgcggcaa tggcgaggaa cctggcgagc cgcttgcttc ttctcttcca
      241 cataactcac atcgtgttgt ggcgcctgcc gggcgcctcg gcgggccgac gccggccaag
      301 ccgggaagtg gcatgagggg acgaggacac cgatttccgg cgcgccatcc ccggcaccgg
      361 ccagcatagg ccgtcgactc ggctcctggc gacagtgaaa tactgttgtc agcctggatc
      421 aatgggcgta tggccagatg cagattgacc atgaagaaca ccgttttcct cattgcctgg
      481 gcgggcgccg tgtcggtgct cgacaccgcg tgtctcggcc agaacctctt gcccaacccg
      541 agcttcgagc gatcgcagga acgcgtcgag ggatggactc tttcgggcgg caagggccgt
      601 gtcgtgcgcg ctgagggcaa cgctgccgcc ggccaggtct gcattggtct cgaaggcgac
      661 ggcaaggaac ccaactggtg gcggacggcg gatttggcgc tgcagcccgg gggcctatac
      721 tgctttggct tcatgggacg acgcgcggcc gaggcgcacg gggggactgc gatgtccggg
      781 ccgagccggg ttaaccggga tttccccctc gaccatcgtt ggggacgcga ggagttcatc
      841 ttcatgaacc cgcacaacaa cccgcgcgat ttcgtgcgac tcgggcaatg ggaggttgac
      901 gggcgattgg atttcgactg cgtcgaactg gttccggtgt ttgcggcgca cgccgagacg
      961 gctgagggca tcgaattggg cgaggccgag agcgttcgcg ccggggtgta tcggttcgag
     1021 ccgaacctga actggcgcgg ggccaattat caccgtccgc tggtgacgaa tcaggccggc
     1081 ttcaactcga accgctggtt gttctggccg ggcgccgagg tggtctatcg gttcgccgtg
     1141 tccggcagtc cccaaatctc cgcaagcctg cgcgccggca tcaattatca cacgggcggg
     1201 gtgctgcgaa tcgatgcgag ccgggatgga caggcctggg tggctgttgc cgagttcgac
     1261 ggccagaagc ggggcgggcg ggctgaactt ccaatgtcgc tgttcccggc cgaccaggtc
     1321 ctcgtccgtc tgagccaatc gggccaggaa gccggattcc aggtggacac cttcgagtac
     1381 gaggcggcgt tggagaaccc gccgcccgac gccgacggcg agacgctgtt tatcgagtcg
     1441 cgtgaagcga gtccggaggt cggtattgcg ctggccggtt ggacgtgtcg ggccgcggac
     1501 ggggactttt gcatcgcgat tgtcgccacg aatcgcgcgc cgcgcgccgt cgccttcact
     1561 gcatcggcga cgtgtctcgg catcgggcag gaagcgggcc cagcagtccg ctcgatgacg
     1621 gcttcggtgc cgcctggtga gacaggccgc ttcgatctgc gccttgggtt tcccggggcc
     1681 ggggagaatc ggatcgcgtt gacgatcacg aacgacgcgg gccagggcgt ctttcggggc
     1741 acgtcgatgc tccgggtggg gctgctcgag gacgccagct acggattctg gctggcggac
     1801 gagggcggcg tgacagcctg gtggtgcgaa agcggctgga aggtgggacg cgaccgggtt
     1861 gcgccggttc cggcgcggat gggagagcgg cgggcgatcg agatcgccgc ggcccggaac
     1921 gaattcgagg ccgtgcaact ggtgttgcgg ccggatcgcg agacgaccct gctcgaaact
     1981 cccgccgtga ccctgcgtga cgagcgcggg gattcgagtc ccattgaagt tcgtttcgac
     2041 gaagtggcgt acgtgcatgt gacccagccg acggacgcct cgtgtgttcg gggttggtat
     2101 cccgatccgt tgccgccgct caagaccccg ctgaaactgg cggctcaccg caaccagccg
     2161 ctctggatca ccatccgggt gccggagtcc gcccgggccg gacgccaccg cggcgagatc
     2221 gaggtcagga cgtcggcggg gacactgcgg gcggggctcg aggtcgatgt gtacgatttc
     2281 gccctgcctg cggagacgca tctgcgcagc gcgttgggct tgggaacgca cgaaatcaac
     2341 cggtatcacc gcctgagcca tggggtagac aaggaggcgg tgttcgccaa atacctgcga
     2401 aattttgccg agcatcggat cagcccgtat tcgtttttcg agtatgcacc gtttcgtgtc
     2461 cggttcgagg gtgagggggc gaacaagcgg gcgcgggtgg atttcgccca gttcgatgtg
     2521 gcagcgcgga catggctcga tcagcatcat ttcagcacgt tccagcttcc gttgcggggc
     2581 atgggcgggg gcacctttca cagccggcat ctcggggaac tcgaaggatt ccaggagggc
     2641 acgcccgagc atgcggcgct attcagggat tacctggggc aggtcgagcg gcatctgcgc
     2701 gagaacggtt ggctgggcaa agcgttcacc tattggttcg atgagccgga tccgaaggac
     2761 tacgcgttcg ttgtggcggg ccagcagcgg atcaaggaag ccgcccctgg cctgcgccgg
     2821 atgctgaccg agcagccgga gaaggagttg ataggccatg tggacatctg gtgcggcctg
     2881 actccggaat ggacacgcga gagggtgcgg gcgcgcaagg aggccggcga ggaggtctgg
     2941 tggtatattt gcaccgggcc caaggcaccc tacgtgaccg agttcatcga tcatcccggc
     3001 accgaactgc ggctctggcc ctggcaatcg tggcagtacg gcgtctcggg catcctggtc
     3061 tgggccacgg tgtactggag cagtccgttg gcctatcccg agcccaacgt gcagaatccc
     3121 tgggccgatc ccatgagctg ggtctccggt tacggcaacc cggtcggcta ccgcaggccc
     3181 tggggcaacg gcgacggccg cttcctgtac ccgccgcggc gcgatcccaa cgcctcgacc
     3241 gcgccgaacc tcgatgcccc gatcaactcg cttcggtggg acaacctgcg ggacggcatg
     3301 gaggactacg agtacctctg gctgctcgaa cgggaggtcg agcgcgtcgg cgatcgcgag
     3361 gcatcgagcg cgttggtccg agaggctcgg agtctgctcg aggttccgga gagcgtttcc
     3421 aaggacctga cgcacttcac gaccgatccg cggcccattc ttgcgcatcg cgacaaggtc
     3481 gcgcgcatga tcgagcgttt gcgtctgatc gaatgacgcc gtccgattgc gtcgtctgtc
     3541 acttcgagcg gaggcggtag aatcgagccg gaaaatccgt ccacttcggg tcgttgaaag
     3601 acgaaaggcc cgtggcgagt gtgttcgtgc tcacgggcac ccagatcgga ttggccggat
     3661 cagagcaggc ttcgacgacc acaatgctct tgggggtcca tgtgatgtcg aatccgaagc
     3721 ggttcgcgtg gacgccgaag tcggcgtcgg cggcctcgat ccggggcacc cacacggcgg
     3781 tcggacgccc gctgaatgtc ggctcccaac cggtggtctc cggcaggtaa tagaccgttg
     3841 cggggcaatc gtcgaatgcg gtggacgtgg cgggggcgtt tccgaggaag cagacgctgg
     3901 ccagtccggt gcagcccgcg aacgcgtgct ctccgatgct ggtgacttta ggcgggagcg
     3961 tgatccgcgt caggtcggtg cacccgaaga acgccccatc cgcaatgcgg gtcacggtgt
     4021 cggggatcgt gacgcttgtc aggccggcgc aatcggaaaa caaccacgca tcgatgctgg
     4081 tcaccccttt ggggatcgtg acgcttgtca ggccggtgca gcttccgaac gcatacggtc
     4141 caatgctcgt gacgctgggc ccgatcgcga cgtccgccag gccggtgcag gcaaagaatg
     4201 cataccgccc aatgctggtg acacggttcg ggatggcgat ggcggtgagg ctggcgcagc
     4261 ggcagaacgc gtacggcccg atgctcgtga cgcggctggg gatggtgatg ctcggcagac
     4321 tgctgcaacc gtagaatgca tacgccccga tgcgggtgac actctcgggg atcgtgacgc
     4381 tcgtcagact cgtgcagcgg tagaagaccg cgactccgat ggtggtgatg ctgctgggga
     4441 tcgtgacgct cgtcaggctc gtgcagctct cgaacgcctc gtctccgatg gttgtgaccg
     4501 tgttggggat cgtgacgttg gccaggttgg tgcaagccca aaacgcccac gcctggatgc
     4561 tggtgacgct cctggggatc gtgatgctcg tcaggctcgt gcagcggcaa aacgcgtatc
     4621 gcccgatgct ggcgaccttg gttcccagtg tgacgctggc caggttgacg caatctgcaa
     4681 acacctcgtt ccccagactt gtgaccgtgt tggggatcgt gatgcccgtc aggctggtgc
     4741 agcgcgcaaa cgcggctcgt ccgatggtgg cgacctttgt cccgagtgtg acgccggcca
     4801 ggttgacgca atctgcgaac acctcgttcc ccaggcttgt gaccgtgttg gggatcgtga
     4861 tggcggtcag gctggtgcag cccgcaaacg cggcccgtcc gatggtggcg acctttgtcc
     4921 cgagcgtgac gccggccagg ttg
//
LOCUS       DAOQRO010000274         4903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18079_length_4903_cov_4.932343, whole genome shotgun sequence.
ACCESSION   DAOQRO010000274 DAOQRO010000000
VERSION     DAOQRO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18079_length_4903_cov_4.932343"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKK20_10135"
     CDS             complement(<1..720)
                     /locus_tag="PKK20_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNV00288.1"
                     /translation="MPKQPVSENPSPAALGFAMPAEWEPHEATWLGWPYHPTDWPGKL
                     DAIRWVYGEMVRKIAPGEIVRILVRSRGEQALARRYLERARADMRQIEFVMHPTNRGW
                     TRDSGPIFVRRRRPRRETAIVHFHFNAWAKYHDWQKDRRVPETAAKLLRKRLFNAEHD
                     GRDFVIEGGGIEVNGRGTLLTTEECYLHPTVQVRNPGLGRAEIESTLERYLGVTRFGW
                     LAAGPVGDDTHGHIDDICRFVN"
     gene            858..2156
                     /locus_tag="PKK20_10140"
     CDS             858..2156
                     /locus_tag="PKK20_10140"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00289.1"
                     /translation="MNAVTASVLPRRQFLTRAFAVTAPLILPGAVLGLNGATPPSEKI
                     VFGGIGMGNRALYILPNFLSQPDLHFAAISDAREDRRQAGKKAIDAFYRNSDCRVYHD
                     FRELLAQKGIDAVLIATGNRWHATASMFAARAGKDIYCEKPISLTIREGRELVDLCKR
                     YGAIYQAGTQRRSTESYQFARELVQQGKIGKIHTVEMQVWTGGTIPHQPNTRVPDGWD
                     YDQWLGPVQWRPFNPGRVNGWAYFWDTGEGMHTDMGTHYTDQMQWVLGTDHTMPVEFE
                     TSDMVWPDPKTWMSETAITGTFKCRYANGVQGILYQRKGFTDRYIRYIGDAGWVQVDD
                     ETDVVTAEPKSILQFRPEGGVSWSNASSHIRNLLACIRSRRQPLCHPEVAHRAQAIVE
                     SMTIAARLNRRLGWDAVEEVFDCSEANQMLYREPRAPWRV"
     gene            2312..4300
                     /locus_tag="PKK20_10145"
     CDS             2312..4300
                     /locus_tag="PKK20_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV00290.1"
                     /translation="MKTQFVPAIAWLVGAAMLQAADTAGSNLEALRQYESGSNVAVLR
                     HYEQQVAQAIDDPAARQKVESELVEVLAGQATFEARRFVCTQLAIIGSKASVPATAKL
                     LESDETVGIACLALAKNPSIQAGQVLRETLGRLKGQPLVQVINTLGTRRDREAVDALL
                     EFAKSEDRAVAGAAYLALGKIGGSSARIAMAEARQKNDPAVADAVAAGSFLFAERLAA
                     QNRRPGAVRIYDQLLAPDHADHVRRGAFEALLRLDADGGLQRATGALTGADAIFKPSA
                     IAAVATMQNAETSKSFAALLPKLEPTHQALLLEALAERGDAEARKAVAAEVDSPHALV
                     RSAAIVNLGRIGTASDVPLLAQATQAAKGAEELKLIETALAGLQGGAAVDQALIAQLR
                     NRMAGPKTPFLAALVRRANPSSLRVFLVEAGSTSPDMVKLAFQGLTRVGTAEDMPAVL
                     KALGGLRAESVRAEAMEATSQLLSRVGTPSANAGAIRAALEAAPSSSGTQTYLPLLAV
                     CPDADGLVLVARSAADADASVRDIGLRTLADWPDASAWGPLSDCYSKAATETERVLVL
                     RGLTRLLGEQNAHPDSEVVARYRALLAGAKTETDRKLILGALAGCHHPDALTVAVEQV
                     AQPGAQGEAKLTVRRIAEAIKAQHPEAAAAALKQIEGK"
     gene            complement(4278..>4903)
                     /locus_tag="PKK20_10150"
     CDS             complement(4278..>4903)
                     /locus_tag="PKK20_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNV00291.1"
                     /translation="SRVVRNSRIYGGLGMVPVFMIGLYVAWVILLLGAQVAYAYQNRR
                     TYLQEKQVEGIHQRGREFVALRLATEIARHYHQANGPVSVATLAERVGVPTSLTGQLL
                     RALTRAKLLVETTDTDTGYLPARPPDRITAQDVLQAVRAGHGMELATREDPGRALVRD
                     EFERIAAAERDAATGVTLQTLAKRVTEAGQSSQAARDGDPATCPRSA"
BASE COUNT          822 a         1538 c         1705 g          838 t
ORIGIN      
        1 gttcacgaag cggcagatgt catcgatatg gccgtgggta tcgtcaccca cggggccggc
       61 ggccagccag ccgaagcggg tgacgcccag atagcgttcg agggtcgatt cgatttccgc
      121 gcggccgagt ccgggattgc ggacctggac ggtggggtgg aggtaacatt cctcggttgt
      181 aaggagggta ccgcgcccgt tgacctcgat gccgccgccc tcgatcacga aatcgcgtcc
      241 gtcgtgttcg gcgttgaaga ggcgcttgcg gaggagttta gcggcggtct caggcacgcg
      301 acggtccttt tgccagtcgt ggtacttggc ccaggcgttg aaatggaaat ggacgatcgc
      361 ggtctcgcga cggggacgcc ggcgccggac gaagatgggg ccgctgtcgc gggtccagcc
      421 tcgattggtc gggtgcatga cgaactcgat ctggcgcatg tcggcgcgcg ctctctcgag
      481 gtagcgccgc gccagggcct gttcgccgcg ggaccggacc aggatgcgga cgatttcgcc
      541 cggggcgatc ttgcgcacca tttcgccata gacccagcgg atggcgtcga gtttgcccgg
      601 ccagtcggtt ggatgatacg gccagccgag ccaggttgcc tcgtgcggtt cccactcggc
      661 cggcatcgca aagcccaacg ccgcggggga cggattttcg gacacaggtt gttttggcat
      721 cgtcacgcga cctcatgata ggggtgccgg ccaggatgtc caggatgtcc aatagcttct
      781 gactttacat tcggggcggt gttgggaact ctgcgtccag cacgatggcc cctgttcata
      841 gtgaaaggat tccgccgatg aatgctgtca ccgcgtctgt cctgccgcgc cgtcagtttc
      901 tgacccgagc gtttgccgtc acggctccct tgatactgcc gggggcggtg ctgggtctca
      961 acggcgcgac gccgcccagc gagaagatcg tatttggcgg gatcggcatg ggcaatcggg
     1021 cgctgtacat tctgccgaat ttcctttcgc agcccgacct gcatttcgcc gcgatcagcg
     1081 atgcgcgtga ggaccgccgc caggcgggca agaaggccat cgacgccttt tatcgcaact
     1141 cggattgccg ggtctaccac gatttccgcg agctgctggc gcagaagggc attgacgctg
     1201 tgctgatcgc cacgggcaac cgctggcacg ccaccgcctc gatgtttgcg gcgcgggcgg
     1261 gcaaggacat ttactgcgag aaaccgatca gcctcaccat ccgggagggg cgcgagctgg
     1321 tcgatctctg caagcggtac ggggccattt accaggccgg cacgcaacgc cgctcgacgg
     1381 agtcgtatca gttcgcccgc gaactggtgc agcagggaaa gatcggcaag attcacaccg
     1441 tcgagatgca ggtctggacg ggcgggacga ttccgcatca gccgaatacc cgggtgccgg
     1501 acggctggga ctatgatcag tggctgggcc cggtgcagtg gcgcccgttc aatcccggtc
     1561 gcgtcaacgg atgggcctac ttttgggaca cgggcgaggg gatgcacacc gacatgggga
     1621 cgcactacac ggatcagatg cagtgggtgc tcggcacgga ccacacgatg ccggtcgagt
     1681 tcgagacgag cgacatggtc tggcccgatc ccaagacgtg gatgagcgag acggccatca
     1741 ccggcacgtt caaatgccgg tacgcgaacg gcgtgcaggg gattctctac cagcgcaagg
     1801 gtttcaccga tcgttacatt cggtacattg gggatgcggg ttgggtgcag gtggacgatg
     1861 agacggacgt cgtgacggcc gagcccaaat ccatactcca attccggcct gaaggcggcg
     1921 tgagctggtc caatgccagc tcgcacatcc gcaacctgct cgcgtgcatt cgcagccggc
     1981 gccagccgct gtgccatccc gaggtcgccc accgcgccca ggcgattgtc gagagcatga
     2041 ccatcgccgc gcggctcaac cgcaggctcg ggtgggatgc ggtggaggag gttttcgatt
     2101 gctccgaggc caatcagatg ctgtaccgcg agccgcgcgc cccgtggcgg gtgtagaatc
     2161 cctgaactcc tgaacatggc acgagcaagc caagtttgcg cctcgctagc ccgcatgttt
     2221 cgtggcgttg tgaccatggc gaagccgaag gctccatcca gaatgtgaac tcatgcgggc
     2281 tcgagagact ccaccgaacc aggttgattt tatgaagact cagtttgtcc cagcgattgc
     2341 gtggttggtt ggtgcggcga tgctgcaagc cgccgacacg gcgggcagca acctcgaggc
     2401 ccttcgccaa tacgagtccg gcagcaatgt cgccgtcctg cgccactatg aacagcaggt
     2461 ggcccaggcg atcgacgacc ccgccgcgcg ccagaaggtc gaatccgagc tggtggaggt
     2521 gctggccggg caggcgacgt tcgaggccag acgcttcgtg tgcacgcaac tggcgatcat
     2581 tggcagcaaa gcctccgtgc ccgctacggc caagctgctc gagagcgacg agacagtcgg
     2641 catcgcgtgt ctggccttgg cgaagaaccc gtcgattcag gccgggcagg ttctccgcga
     2701 gaccctcggc cggctcaaag ggcagccgct tgtccaggtc atcaacaccc tcggcacccg
     2761 gcgcgatcgg gaggccgtcg atgcgctgct ggagttcgcg aaaagcgagg atcgggcggt
     2821 ggccggcgcc gcgtacctgg cgctcggcaa gatcggcggt tcgtcggccc ggatcgccat
     2881 ggcggaggcc cgccagaaga acgatcccgc tgttgcggac gctgtggccg ccggatcgtt
     2941 cctcttcgcg gaacgactgg ctgcgcagaa ccggcgcccg ggcgcggtcc gcatttacga
     3001 ccaattgctc gcaccggacc atgcggatca cgttcgacgc ggcgccttcg aagccctgct
     3061 gcggctggac gcggacggcg gactgcaacg tgcaaccggg gctttgaccg gcgcggacgc
     3121 gatcttcaag ccctcggcca tcgcggctgt ggcgaccatg cagaatgccg agacatcgaa
     3181 gagcttcgcc gctctgctgc cgaagctcga gccgactcat caggcgctcc tgctagaggc
     3241 gctggccgag cgcggggacg cggaggcgcg caaggcggtg gcggcggaag tcgattcgcc
     3301 gcacgcgctg gtccgaagcg cggcgatcgt gaacctgggg cggatcggca cggcgtccga
     3361 tgtgcccctg ctggcccaag ccacccaggc ggccaagggg gcggaggaat tgaagctgat
     3421 tgagactgcc ctggctggac ttcagggcgg cgccgcggtg gatcaggcct tgatcgccca
     3481 actgcgcaat cgcatggccg gccccaagac gccctttctt gccgccctgg ttcgccgcgc
     3541 caacccatcg agcctgcgcg tgttccttgt cgaggcaggc agcacgtcgc ccgacatggt
     3601 caagctggcc tttcaaggcc tgacgcgcgt tgggacggcg gaggacatgc cggccgttct
     3661 gaaggcgctg ggcggcctgc gggccgagtc ggtccgggcg gaggccatgg aagccaccag
     3721 ccagttgctg agccgtgtcg gcacgccatc cgcgaacgcc ggcgccatcc gtgccgcact
     3781 cgaagccgcg ccgagctcga gtggcacaca gacatacctg cctctcttgg cggtctgtcc
     3841 ggacgccgac gggcttgtcc tggtggcaag aagcgccgcg gacgccgacg cttcggttcg
     3901 cgacattggg ttgcgcacgc tcgccgactg gccggacgcc tcggcgtggg gaccgctctc
     3961 ggactgttac tcgaaggcgg ccactgagac cgagcgggtg ctggtcctgc ggggcttgac
     4021 ccggctgctc ggcgaacaga acgcccaccc cgattcggag gtcgtcgccc ggtatcgcgc
     4081 cctgctggcc ggggcgaaga ccgagacgga tcgcaagctg attctgggcg ccctggcggg
     4141 atgtcaccat ccggatgcgc tcactgtggc ggtcgagcaa gtcgcgcaac cgggggcgca
     4201 gggtgaggcg aagctgacgg ttcggcgaat cgccgaagcc atcaaggcgc agcatcctga
     4261 ggccgctgcc gccgcgctca agcagatcga gggcaagtag cggggtctcc gtctcgagcg
     4321 gcctggctcg actgtcccgc ctcggtgacc cgtttggcca gggtttgcag cgtcacaccg
     4381 gtggcagcgt cgcgttcggc ggcggcgatg cgttcgaact cgtcccgcac caaagcgcgt
     4441 ccgggatcct cccgcgtcgc caactccatg ccgtgtccgg cgcggacggc ttgcaggaca
     4501 tcctgggctg tgatgcgatc gggtgggcgg gccgggagat agcccgtgtc ggtgtcggtg
     4561 gtttcaacca ggagtttggc gcgggtcaag gcgcgcagga gttgtccggt gaggctggtg
     4621 ggaacgccga cccgctcggc gagggtggcg acgctgaccg gtccattggc ctggtgataa
     4681 tggcgggcaa tttccgtggc cagccgcaga gccacgaact cgcgtccgcg ctggtggatt
     4741 ccctcgacct gtttctcctg gaggtaggtg cgccggttct gatacgcgta ggccacctgg
     4801 gcgcccagaa gcaggatgac ccatgccacg tagaggccga tcatgaacac cggcaccatg
     4861 cccagcccgc cgtagatgcg gctgtttctc accacgcgcg aga
//
LOCUS       DAOQRO010000275         4900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18093_length_4900_cov_3.611765, whole genome shotgun sequence.
ACCESSION   DAOQRO010000275 DAOQRO010000000
VERSION     DAOQRO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18093_length_4900_cov_3.611765"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..636)
                     /locus_tag="PKK20_10155"
                     /pseudo
     CDS             complement(103..636)
                     /locus_tag="PKK20_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
     gene            complement(668..1144)
                     /locus_tag="PKK20_10160"
     CDS             complement(668..1144)
                     /locus_tag="PKK20_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNV00292.1"
                     /translation="MRPSNIAEGSQASGTSKKMELKLTNVARASLEELRLDYEDFLRQ
                     RRLALYRPNHPALMRFKARRCATLDEVRAWVAEEVGKARTDSDGHKASKGVNVSERPC
                     VSVPKPEELVANAALSLLNLACYFLNRQLAAQAEAFEKEGGFTERLYRVRQQQRRH"
     gene            complement(1354..1995)
                     /locus_tag="PKK20_10165"
     CDS             complement(1354..1995)
                     /locus_tag="PKK20_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNV00293.1"
                     /translation="MSDDGNNAKKGTPGEKPSPDSHPSHEDHPIIPPRGDYQTLHSFH
                     KAEVVYDLTFRFAHKHLAKGDRTIDQMIQAARSGKKNLLEGSKAALTSKETEIKLTSV
                     ARASLEELLDDYRDYLRVREHREWDKDSREALYVRRLGRVVPQTYELYREFLDTRPPE
                     VVANIAICLIHQANYLIDQQLRRLEKDFLEQGGLRERMFQARLQHRRRHPPKP"
     gene            complement(2132..2207)
                     /locus_tag="PKK20_10170"
     tRNA            complement(2132..2207)
                     /locus_tag="PKK20_10170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2172..2174),aa:Val,seq:tac)
     gene            complement(2441..2932)
                     /locus_tag="PKK20_10175"
     CDS             complement(2441..2932)
                     /locus_tag="PKK20_10175"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNV00294.1"
                     /translation="MAQPDPFHVVLLEPEIAPNTGNVARLCAATRTCLHLIEPFGFEL
                     DDRRLQRAGMDYWQHVEWRRWPSWAEFRGQVRSDARLWFIESHGPRLYAEAAFARGDY
                     LVFGRETAGLPAALLAAHREWWLRLPMFHPRARSLNLANCVGIVLYEALRQQGFAGEI
                     GPS"
     gene            complement(3019..3819)
                     /gene="truA"
                     /locus_tag="PKK20_10180"
     CDS             complement(3019..3819)
                     /gene="truA"
                     /locus_tag="PKK20_10180"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNV00295.1"
                     /translation="MTGARVRLKLTIAYDGTGYEGWQIQKTGLGVQQRIEEALARLFA
                     SHPRVHGSSRTDAGVHAVGLVAHLDVPRDEWRMTGAKLRLAMNAHLPEDIRVLSAARC
                     RPSFHARFDAVRKQYRYLVWNHPAMNPLWRHRAWHVPRTLDAAAMRAAAVSLVGRRNF
                     QSFTANPGYGRASSVRTVFRCDVRRHGPMLTFIIEADAFLYKMSRGIVGTLVQVGLGK
                     IKPSDMSGILECRDRRVAGMTAPAHGLVLWKVTYGRKARPGRSDSGLG"
     gene            complement(3915..>4900)
                     /locus_tag="PKK20_10185"
     CDS             complement(3915..>4900)
                     /locus_tag="PKK20_10185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV00296.1"
                     /translation="ASGDVAAARTWIDAGLAPLLEGDPDLATLIPFDRHRWRTPRIWV
                     DAGRELARMRRARYDLVIDLQALARSSLIAWLARGRLTIGLDDTREGASALYDIRVPR
                     PSPTTHAVDWYLAVLRLLEIPVHRDFVWLPERVEVADRIRARWPGGQMDWVGISPGAR
                     WENKRWPAERYAALVARLAEECPQLRFAVLGAASDRALAEAVCAGRPDRCVNLAGQIS
                     LPEMVEWIRRCRVLVSNDTGPMHVAAALGTPVVALFGPTDPRRTGPYDQVDHVLQHPL
                     ACVPCLKSRCSHAPPLECLRLISVDQVCEAVRQRLGEPSGVATRDSHRESQ"
BASE COUNT          874 a         1624 c         1513 g          889 t
ORIGIN      
        1 ttggacgttc ggattacccc ttttgttaat tatcaacggc ttgcgcaggc ggttcctgga
       61 aagggccagt cgaacttcgc gcgagaagtg cgatgcgcac gaatcagcca cttcgctggc
      121 ctcgagatcg atgatgcgct gcatctcgga gagttggtcg tggccgaacc ccttctcagc
      181 cagcccctgc aacagtctct tgcgggtgtc cggcgcgctc caaatccggc gcaactcgtc
      241 ctcgtcgtgg aagaactccg gcagcctgcc aaagagcaat tccatgaact gttgcgcgct
      301 catgggcgtg ccgtcgggat gccagaagct cgtgcacgtc atgtgctgga tggtccgctc
      361 tttgccatcc gccagcttca ccttcacttt cttccggcac acgcacggac gctgaccgca
      421 cgtcgggcag ggttcgggcg cctcgggtgg ttcgggctcg ccatcccact cggggtccag
      481 gaaatgctgg tgcgccttca cgaagtcgta aatcgtgaag taatccttcc catcgaatag
      541 ccgcgtgccg cgcccgatga tctgcttgaa ctcgatcatc gaattcaccg gccgcatcaa
      601 cacgatgttg cggatgttct gcgcatccac gccggtcggc cttcgtctcg gcttcgttca
      661 tggcactcta atgtcggcgc tgctgctggc gcacacggta gagccgctca gtgaaacctc
      721 cctctttctc gaaggcttcc gcttgggccg ccagttgccg gttcaggaag tagcatgcca
      781 gattcagcag cgagagcgcc gcgttggcca caagctcctc cggcttcggc acagacacac
      841 acggacgttc agagacattc acgcccttcg acgccttgtg tccgtccgag tccgtccgtg
      901 cctttcccac ctcttccgcc acccaggctc gcacctcatc gagcgtagca cagcgccgtg
      961 ccttgaagcg catcagcgcc gggtggttcg gccggtagag cgccaatctc cgctgccgaa
     1021 gaaaatcctc atagtccagc cgcaattcct ctaggctggc ccgcgccacg ttggtgagct
     1081 taagttccat cttcttggac gtcccgctgg cctggctgcc ctccgcaata tttgaaggtc
     1141 gcacctgtac cgagcgagtc gagcaactcg attatgctgc tgagatagta atacagaaca
     1201 gcagcctcat gattttcgcg ggcacctcgg cactttgggc ggcgggggtg gcgggggtgc
     1261 gtaggcggcg gcacatttgt cccatagctc ccatgccccg caaaacgcgc gggcgggccg
     1321 cgggggaaac gggcggcatg gcctgagtgt gggtcaaggc ttcggcgggt gtcggcggcg
     1381 atgttggagg cgcgcctgga acatgcgctc gcgcaggccg ccctgttcca ggaagtcttt
     1441 ctccaagcgg cggagctgct ggtcaatcag gtagttcgct tggtggatga ggcagatggc
     1501 gatgttggcc accacctccg gcgggcgcgt gtccaggaac tcgcggtaca gctcgtaggt
     1561 ctgcgggacc acacgcccga gccggcggac gtagagggct tccctggagt ccttgtccca
     1621 ctcgcggtgc tcgcgtacgc ggaggtagtc gcggtagtcg tccagcagtt cctccaggct
     1681 ggcgcgggcg acactggtga gcttgatctc ggtttccttg gaagtgaggg cggctttgct
     1741 gccttcgagc aggttcttct tgccggagcg cgcggcctgg atcatctggt caatggtgcg
     1801 gtcgcctttg gcgagatgct tgtgcgcgaa gcggaaggtc aggtcataca ccacctcggc
     1861 tttgtggaac gagtgaagcg tctggtaatc gccgcgcggg gggatgatcg gatgatcttc
     1921 atgggaggga tgggaatcag gcgacggctt ttcgcccggg gtccctttct tcgcattatt
     1981 cccatcgtcg ctcataccag tttcctgatg cccgccagca cctccgagct cctcgcatcc
     2041 agcgcggcga cttcctttat gatctcctgc gggtggcggt gggtcacggc ctcgccgccg
     2101 ttggggttct tctcattttc ctcagcaaat ctggtgggcg atgcaggatt cgaacctgcg
     2161 acctactccg tgtaaaggag acgcgctagc cactacgcta atcgcccaag cccttccctg
     2221 accataaaca cccacccctg cttggcaact gagaaaggcg ctccccttct ggttatctgc
     2281 gccaattcat gaagccgcaa aggccggagc ggggacggct cgtccccagc caacacgagt
     2341 gaagacggta tttgccggcg gcagacaggc gtcgccgctc cacttcatgc attggcccag
     2401 tctggtcatc tgcgcctgtg ccgcgccgtg aagaatctga ctatgatggc ccgatttctc
     2461 cggcgaatcc ctgttgccgc agtgcctcgt aaagcacgat gcccacgcag ttcgccaggt
     2521 tcaacgaccg ggcccgcggg tggaacatcg gcaatcgcag ccaccactcc cggtgcgccg
     2581 cgagcagcgc cgccggcaac ccggcggtct cgcgtccgaa caccaagtaa tcaccccggg
     2641 caaaggccgc ttccgcataa agccgcgggc catgcgactc gatgaaccag agcctggcat
     2701 cggatcgtac ctggccgcgg aactcggccc aacttggcca acggcgccac tcgacgtgct
     2761 gccaatagtc catgcccgcg cgctgaagtc ggcggtcatc caactcgaaa ccgaacggct
     2821 cgatcaggtg caggcaggtc cgcgtcgccg cgcagagccg ggctacgttg ccggtattgg
     2881 gcgcgatctc gggttcgagc aacaccacgt gaaacggatc gggttgtgcc atagttggca
     2941 ttgcatgcgg ctcggaagcc ttagggtctg tgcaagaatc aatcggaagt agtttttgca
     3001 cagaccctta acccggaatc atcccagccc ggaatcgctc cggcccggtc gagccttccg
     3061 cccatacgtc accttccaca agacaagccc gtgcgccggc gctgtcatgc cggctacgcg
     3121 ccggtcgcgg cactcgagaa tcccgctcat gtctgacggc ttgatcttcc caagcccgac
     3181 ctgcacaagc gtacccacaa tgccgcgaga catcttgtaa agaaaggcat cggcctcgat
     3241 gatgaacgtt agcatcggcc cgtgccggcg cacgtcacaa cgaaacacgg tccgcacgga
     3301 cgaggcgcgt ccgtaaccgg ggttggctgt gaaggactgg aaattgcgcc gcccgacgag
     3361 cgatacggcc gcggcgcgca tggcggcggc atctagcgtg cgcgggacgt gccaggcccg
     3421 gtggcgccag agagggttca tcgccggatg attccagacc aggtagcgat actgcttgcg
     3481 cacggcatcg aaccgggcat ggaaactcgg acggcagcgc gcggcgctca ggacgcggat
     3541 atcctcgggc aaatgagcgt tcatcgccaa acgcagcttg gcgccggtca tccgccactc
     3601 atcccgcggc acgtccagat gcgccaccaa gcccacggcg tgcaccccgg cgtcggtccg
     3661 gcttgatccg tgaacccgcg gatgcgacgc gaacaaacgg gccagcgctt cctcaatgcg
     3721 ctgctgaacg ccaagaccag tcttctggat ctgccagcct tcgtacccgg tcccgtcgta
     3781 ggcgatcgtc agcttgagac gaacccgggc accggtcact gctggaacct tcgcgccctg
     3841 ctcaaccgcc cgtcgtcccg acgcgccgat gtgtcggcta tcgagggtcg aatgccccgg
     3901 gtcggacata tgcttcattg cgattcccga tgcgaatctc gagttgcgac tcccgagggc
     3961 tcgcccagac gctgccgaac cgcctcgcac acctggtcga ccgagatcaa tcgcagacac
     4021 tcgaggggag gcgcgtgaga gcatcggctc ttgagacacg gaacgcatgc caacgggtgt
     4081 tgcaggacgt gatcgacctg gtcgtacggc ccggtgcgcc ggggatcggt cggcccgaag
     4141 agggcaacca cgggcgtgcc cagagcagcc gccacgtgca tcggaccggt gtcgttgctg
     4201 acaagcaccc gacagcggcg tatccactcg accatttccg gcaagctgat ctgccccgcc
     4261 agattcacgc atcgatccgg acgcccggcg cacaccgcct cggccagcgc acggtcgctg
     4321 gctgccccaa gcaccgcgaa ccgaagctga ggacactcct ccgccagacg cgccacgagc
     4381 gcggcgtagc gctcggccgg ccaccgtttg ttctcccatc gcgccccggg cgaaatgccc
     4441 acccagtcca tctgaccgcc aggccagcgc gcgcggattc gatcggcgac ctcgacccgc
     4501 tcgggcagcc agacgaagtc gcggtggaca ggaatctcga ggagcctcag caccgccaga
     4561 taccaatcca ccgcatgggt cgtcggtgag gggcgtggga cgcggatgtc gtagagggcc
     4621 gacgccccct cccgggtgtc gtccaagccg atcgtcagac gcccccgcgc cagccaggcg
     4681 atcaggctgc tgcgcgcgag cgcctggaga tcgatgacca ggtcgtaccg cgcccggcgc
     4741 attcgggcaa gttcgcgccc ggcatcgacc caaatccggg gcgtgcgcca tcggtgacga
     4801 tcgaacggaa tcaacgtcgc cagatccgga tcgccttcga gcaacggcgc caggccggcg
     4861 tcgatccaag tgcgtgcagc ggcgacgtcc ccggacgcga
//
LOCUS       DAOQRO010000276         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18121_length_4894_cov_4.176896, whole genome shotgun sequence.
ACCESSION   DAOQRO010000276 DAOQRO010000000
VERSION     DAOQRO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18121_length_4894_cov_4.176896"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKK20_10190"
     CDS             complement(<1..840)
                     /locus_tag="PKK20_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV00297.1"
                     /translation="MQSVSDIHKVAFVGNYLPRKCGIATFTTDLCNAVDRLGPRFECA
                     VVSVNDLAEGYSYPPEVRFEMQEQHLPSYQQAADFLNLTNADVVCVQHEFGIFGGPAG
                     SHLLSLLRGLRAPVVTTLHTVLKDPNEDQRRVMRDLVRLSTRLVVMSAKGEVFLREIY
                     AAPASKIDLIPHGIPDMPFVDPNYFKDEFGVAGKKVLLTFGLLSPNKGIEYALQALPD
                     IVREFPEVVYVILGQTHPNLLREQGDAYRLSLERLAKDLAMEKHVVFFNRFVELEELK
                     VFIG"
     gene            complement(1202..2548)
                     /gene="purD"
                     /locus_tag="PKK20_10195"
     CDS             complement(1202..2548)
                     /gene="purD"
                     /locus_tag="PKK20_10195"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNV00298.1"
                     /translation="MKLLVLGSGGREHALVWKLAQSARARQIWCAPGNAGIAAERLAA
                     NDAPVECVPIGAEDLEPLLAFARQHRPDLTVVGPDNPLAAGIVDLFEQNGLRIFGPNR
                     SAARFESSKVFAQEFMERHGIPTARAGTFTEFAAASRFASGLGGRCAVKADGLALGKG
                     VLICRNMEQADQAIEQILVQKAFGPAGARIVIQELLEGTEVSLHALCDGKTARLFPTS
                     QDHKRALDGDQGPNTGGMGTCSPVPFLSEADLASIRRSILEPWLRGCAADGIDYRGIL
                     YPGLMLTRDGPKVLEFNARFGDPETQVYLMRLESDLADLLDACVSGALDRIDITWRPE
                     SAVCVVMASGGYPGSYPKHKPIQGLDSAGRLPNTKVFHAGTAHEAGRVVTNGGRVLGI
                     TALGADLEKARAAAYAAVREIRFDGAQFRRDIGMKALARVSHQLLMSSDRSPSHFT"
     gene            2905..3063
                     /locus_tag="PKK20_10200"
     CDS             2905..3063
                     /locus_tag="PKK20_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00299.1"
                     /translation="MTSTACYAHLIHINAYHQLGVQAGKIAADKVLELQQRVVAYLAG
                     APDCKPAP"
     gene            3328..4395
                     /locus_tag="PKK20_10205"
     CDS             3328..4395
                     /locus_tag="PKK20_10205"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009784997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNV00300.1"
                     /translation="MSKLVLAGDIGGTKTILQLVHAQAPAPGHSRPSLATLAEKTYHS
                     RDYPDLAPMVKRFLDESESALDGRGEVEHACFGIAGPVVNQASQLTNLSWSLSAERLQ
                     RDLRIGRVTLLNDFSAIGYGLAGLDDSALLTLQKGTPDRSAPIAVIGAGTGLGEGFLV
                     PDASGRYRTFPSEGGHTDFPSRSALEAQLLDHLREHLRVPQISVERVVSGPGIALIYQ
                     FLCKQHPEQETPAMARVREQSLLETDGETSTVDLSAAVSEAALSGGDALCLQAMRMFV
                     SAYGAEAGNLALKLLCKGGLFVAGGVAPKILPLLQDGTFLEAFLNKGRMRSLMEAIPV
                     RVVLNPKVGLIGAALCAGQDS"
     gene            complement(4471..>4894)
                     /locus_tag="PKK20_10210"
     CDS             complement(4471..>4894)
                     /locus_tag="PKK20_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNV00301.1"
                     /translation="RFVGQAHPVAVNQILASPRARDFAEHRMFPDEILFCGAMSVYVP
                     YADPGLPLAWQIRERTRDFIREHGMAPRLILLQNHGIVALGASAPEVLATVMMANKAA
                     LIFSGAAAMGGPTFLLPQHVDRILARPDEGHRQRLVDF"
BASE COUNT          913 a         1602 c         1528 g          851 t
ORIGIN      
        1 gccaatgaac accttgagtt cctccaactc gacaaagcgg ttgaagaaca ccacgtgttt
       61 ttccatcgcc aggtccttgg ccaaccgctc caggctcaac cgataggcat ccccctgttc
      121 ccgcagcagg ttgggatgcg tctggcccag gatgacatac acgacttccg ggaattcgcg
      181 cacgatgtcg ggcagggctt gcagggcata ctcgatgccc ttgttgggcg agagcaggcc
      241 gaaggtcagg agcaccttct tcccggcaac accgaactcg tccttgaagt agttgggatc
      301 cacaaacggc atgtcgggaa tgccgtgcgg gatcaggtca atcttgctcg ccggggccgc
      361 gtagatttcg cgcagaaaga cctcgccctt ggcggacatg accaccagtc gagtggacaa
      421 ccgcaccagg tcccgcatga cccgccgctg gtcctcgttc gggtccttca gcaccgtgtg
      481 caaggtggtc accacgggcg ctcgcagccc gcgcaggagc gacagcaagt gactacccgc
      541 cggcccaccg aagatgccga actcatgttg cacgcatacc acgtcggcgt tggtcagatt
      601 caagaaatcc gccgcctgct ggtaggaggg caggtgctgc tcctgcatct cgaaccgcac
      661 ttccggcgga taggaataac cctcggccag atcgttgacg gacacgacgg cgcactcaaa
      721 gcgcggtccc aaccggtcca ccgcgttgca cagatccgtc gtaaacgtgg cgatgccgca
      781 tttgcggggc aagtagttcc ccacgaacgc gaccttgtgg atgtcagaaa cactctgcat
      841 ggctcacagc ataactgaga cggcgcttca acgcgagccc gacacaggct tgttgcgcga
      901 ggactgcggc tccgcccacg aggctgtccg catcattgag gactacggct atcgcggcct
      961 ggaatcacgg gatcaggcgc ttggccaatc gccagaggcg ggtctgaccg tccgactccg
     1021 acctctgctt tggttgacgc atatgcgcga tcgtgggacc tgcgagccga agccccccag
     1081 tcctccgggg cggggcggca aaaccggaag taattcttgc accgaccctg ggaaatatgc
     1141 gggctatacg gctacggttc ctcgcgaagc atgtgaaata tccgttgggc taacccgcat
     1201 ttcaggtgaa atgtgacggt gaccggtcag atgacatcaa cagttggtga gaaacgcggg
     1261 ctaaggcctt catgccgatg tcgcggcgaa attgggcgcc gtcgaagcgg atttcccgga
     1321 cggcggcgta ggcggccgcg cgggccttct cgagatcggc ccccaaagcc gtgatcccca
     1381 gcacccgccc gccgttcgtg accacccggc ccgcttcgtg cgcggtgccg gcgtgaaaga
     1441 ccttggtgtt cggaaggcgc ccggccgagt cgagcccctg aatcggcttg tgcttggggt
     1501 agctgcccgg gtaacctcct gacgccatca cgacgcagac cgctgattcg gggcgccatg
     1561 tgatgtcgat ccgatcgagc gcgccggaaa cacaggcgtc cagcaagtcg gccagatcac
     1621 tctcgaggcg catgagatag acctgggttt ccggatcgcc aaaccgcgcg ttgaattcga
     1681 gcaccttggg accgtcccga gtcagcatca gccccggata caggattccc cgataatcga
     1741 tcccgtccgc ggcgcagccg cgcagccacg gctcgagaat gctccggcga atcgaggcca
     1801 ggtcggcctc gctcagaaaa gggacgggcg agcatgtgcc catgccgccg gtgttcgggc
     1861 cctggtcgcc gtcgagcgcc cgcttgtggt cctgggaggt cggaaagagc ctggccgtct
     1921 ttccgtcgca cagggcatgg agcgagacct ccgttccttc gagcaactcc tggatcacga
     1981 tgcgggcccc ggccgggccg aacgccttct gcacgaggat ttgctcgatc gcctgatcgg
     2041 cttgctccat gttgcggcaa atcaatacgc ctttgccaag ggccaacccg tccgccttga
     2101 cagcgcagcg tcccccgaga ccggacgcga atcgagaggc ggccgcgaac tcggtgaacg
     2161 tccccgcacg ggcggtggga atgccgtgac gttccatgaa ctcctgagca aagacctttg
     2221 aggactcgaa ccgtgcggcg gaacggttcg gcccgaaaat gcgaagaccg ttttgctcga
     2281 agaggtcaac gattcccgcc gccagcgggt tgtcagggcc gaccaccgtc aggtcgggcc
     2341 tgtgctgccg ggcaaacgca agcaacggct cgaggtcctc cgccccgatc gggacgcact
     2401 cgaccggggc gtcgttggcg gccaggcgtt cagcggcgat gcccgcattg ccgggggcgc
     2461 accagatctg gcgcgcacgc gcggactgag caagtttcca aaccagggcg tgctcccgcc
     2521 cgccggagcc aagcacaagc agcttcatgc ggacccaagt gaaacagcaa acagcccgct
     2581 acgaaagcaa ttccgcactc gcctgcatgt ttcacggcgt tgtgaccgcc gcgaagcgga
     2641 tgactccaca atgcgggccg ctgcgggcga gaggtgcatt tctccgttcc caagccgcgg
     2701 cccggtccct tatgcagccg gccggcagca gaacatacga cactatgaac gcgtggcgac
     2761 gattcccgga caactaccat ttttcccgta gatcgatctt gcggttgacc tcagtcgggt
     2821 ggatttcccg cccggctatc acgcctcgat gacggacaag cgagacctct gccgtgggcc
     2881 tgcttccggc cgcgcttcag ggctttgaca tcgacggcct gctatgcgca cttgatccac
     2941 atcaacgcct accatcagct cggcgtccag gccggcaaga tcgcagccga caaggtcctc
     3001 gaactccaac agagagtcgt ggcctatctg gccggcgcgc cggattgcaa gcccgcgccc
     3061 tagacggccg tcgagattgc ccaggcattg ccgtcgccct cagacctcaa gttcgccgac
     3121 cggccgcccc ggagaccgtc ttcaaggtct gcgagcatct ggcggccaac cctgggcgcg
     3181 gagtgcggcg cgtcggtggg cggttgcccg ccacggcccg cttggccgcg gaatagccgg
     3241 catcgcgtgc caccctttct cttgccgcgc agaggcgaaa gggagtacgc tcggcgcgca
     3301 gcttgagcgg gaacaggatt ttgtgctatg agcaaacttg tattggccgg cgacatcgga
     3361 ggaacaaaga cgattctgca actcgtccat gcccaggcgc ccgccccggg ccactcccga
     3421 ccgtcgctgg ccacgctcgc cgaaaagacg tatcacagtc gggattatcc cgacctggcg
     3481 cccatggtga agaggttcct cgatgaatcg gaatcggcat tggacggccg cggcgaggtc
     3541 gagcacgcct gtttcggcat cgcggggcct gtggtcaacc aggcctccca gttgacgaat
     3601 ctgagttggt ctctctcggc cgagcggctg cagcgcgact tgcgcatcgg acgcgtcact
     3661 ctcctcaacg atttcagcgc catcggctat ggccttgccg gactcgatga ttcggccctg
     3721 ctcaccctcc agaagggcac gcccgaccgt tccgccccca tcgcagtgat cggcgccggc
     3781 acaggccttg gtgaggggtt cctggttccc gacgcctcgg gacggtatcg cacgttcccg
     3841 tccgagggag gccacacgga ttttccgtcg cgttcggccc tcgaagcgca gttgctggat
     3901 cacctgcgcg agcaccttcg agtgccgcaa atctccgtcg aacgggttgt gtcagggccc
     3961 ggcatcgccc tgatttacca gttcctctgc aaacaacacc ccgaacagga aacgcctgcg
     4021 atggctcgag ttcgagaaca atcgctgctc gagaccgatg gagagacgag cacggtggac
     4081 ttgtcggctg cggtctccga ggcggcgttg agcggcggtg acgcgttgtg cctgcaggcc
     4141 atgcgcatgt ttgtcagcgc ctatggcgcc gaggcgggca acctcgcgct gaaactcctc
     4201 tgcaagggag gtctcttcgt cgccggcggg gtcgcgccca agattctgcc gctcctccag
     4261 gacgggacat ttctcgaagc attcctcaac aaaggacgca tgaggtcgct catggaggcg
     4321 atccctgtcc gcgtcgtgct caatcccaag gtcggcctca tcggcgccgc cctgtgcgcc
     4381 ggccaggatt cctagcctgc atgctgactt ctcttgagaa ctccggcttc gcggtggcaa
     4441 aaagtggtga gaaatgcagg ctagccgcct tcagaaatcc acgaggcgct gtcgatgccc
     4501 ctcgtcgggg cgcgccagga ttcgatcgac atgttggggc agcaggaacg tcggtccgcc
     4561 catcgcggcc gcgccgctga aaatcagcgc cgccttgttg gccatcatca cggtggccag
     4621 cacctccggc gcggacgcgc ccagggcgac gatcccgtgg ttctgcagca ggattaaccg
     4681 cggcgccatc ccgtgctcgc ggatgaagtc gcgggttcgc tcgcggattt gccaggccag
     4741 gggcagaccg ggatccgcat agggcacata aaccgacatc gcaccgcaga acagaatctc
     4801 gtccgggaac atccgatgtt cggcaaagtc ccgggcgcga ggcgaggcga gaatctggtt
     4861 gaccgcgacc ggatgggcct gaccgacgaa acgc
//
LOCUS       DAOQRO010000277         4867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18264_length_4867_cov_5.983167, whole genome shotgun sequence.
ACCESSION   DAOQRO010000277 DAOQRO010000000
VERSION     DAOQRO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18264_length_4867_cov_5.983167"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..298
                     /locus_tag="PKK20_10215"
                     /pseudo
     CDS             173..298
                     /locus_tag="PKK20_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
     gene            437..1486
                     /locus_tag="PKK20_10220"
     CDS             437..1486
                     /locus_tag="PKK20_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNV00302.1"
                     /translation="MSGKLCSPLLDLAASAILLTGCAVGPKAPLARQTGESVVFIGEE
                     PAALAHLPVRGEPLEVRDTYLPASNSVRYIEGRDFVVDYAAGTLKRLPDSWLPDFRTN
                     MLYGQENFDHTQFPGYGNSGYFAFVDYTHAAPASWPVQDDQTMFLRSTEAKLKAGEAL
                     KIVAFGDSITAGGDASKPDLIYWQRWADALQRKHPDSRITAVNGATGGDSTAQGLERL
                     QTKVLDEKPDLVLLGFGMNDHNVMGVPIPQFESNLIQMITRIRTEVGAEVILFSTFPP
                     NPKWKFGSHRMEEYAAATRRVARETACAYADVFGNWQALAGRKKPEDLLGNNINHPND
                     FGHGIYFQVFEALGL"
     gene            1789..>4867
                     /locus_tag="PKK20_10225"
     CDS             1789..>4867
                     /locus_tag="PKK20_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00303.1"
                     /translation="MRTYSAAALGLILTLTPPFVFGQPPAYGLLREVWEGIPSERLSM
                     LTDSPDYPDRPTSTNYVTDLFEAPTDVLDNYGQRMHGYVVAPQTGNYTFWIASDDNGA
                     LYLSTDENPANARVIATVNSWTSARGWDWEANQRSEPIALIGGRIYYIAALMKEGGGG
                     DNLAVRWLMPDGTDQAPMVATNLLPYGISFAAPVIAEHPANTSAIEGGAARFTVKTAS
                     VGVYSYQWQRNSIALIGATAAELVCDPVTLADHGARFRCVVSNKLGTATSNEGILTVS
                     PDTTPPALVLARNIGLTQVEVTFSERVSAPSATTAANYKIDALGISSAAFGPSSNVIY
                     LTTSPMILGATHTLTVNNVQDAAATPNAVSPNASIQFVVTDFATQDVGNPPLIGDAGY
                     VSGGVDVRGSGDIGGTADALQFAWQKRTGAFDVRARVVRFDSTDPFAKAGLMVREDLS
                     AGSRFVAALATPAAVGCVFMARSAPGAAASRSGYCAANYPETWLRLKRSANLFTAYAG
                     YDGLSWVELGSAQLALPETVYFGLASASRNTAALATASFRDIGAVSQATLMTSAPRGE
                     RLGPSSRQTPLVISEIMYRPLRRPDGKTGEFIELYNADLIAADLTGHRLGGSIQFAFP
                     DGFVLAAGGFAVIARNPVDFAAVYGIADALGPFIGGDGLPNDGGTVRLENPEGATLLE
                     VNYESDFPWPVAADGAGHSLVLVRPSYGEDDPRAWAASDVIGGSPGQMEAVRANPLAG
                     VLINEFLVRPDAAQRGFIELYNHSNNGADVGGCILTDDAQTARFHIAEGTVIPPRGFL
                     AFYEDQIRFGLGGAGGRLFLVNSNATRVLDAVRYGAQERGMAMGRYPDGAPGVRRLIE
                     PSPDNENRSQAIDVIAINEIMYNPISGDDADEFIELHNRGAQRVDLGGWRFTDGVDFQ
                     FPAGTIIEGGGYLVVAKSRLRLLASHPGLDPALVFGDYGGALANGGERVALAKPDLLV
                     LTNAFGLPETNRILIDVDEVRYGVGGRWGQWSDGLGSSLELTDPRSDHSSPSNW"
BASE COUNT          868 a         1482 c         1628 g          889 t
ORIGIN      
        1 taaacgcctc gggaaaagag tgctcgggtt ggtctagact ccgcccggga aagcgggtat
       61 gcgtccaaaa tcactgaggc attgcccggg atgactgagc agcgcctgac cgtgcgcaac
      121 tcacagaata tcaacgcaac acgttgtatc ggcactcgcg cattatcgcc aaaatgtcag
      181 gtctggccag gagctgctgg gccacaagag cgtcgagacg acacaaatct acacgcacgt
      241 gatgcgcaag ccggggttgg gggtgcggag tccgttggat cgggttgatg gtggatagtt
      301 gatagttgat ggatcatagt gttggaatgg ggggagcgag aggaggggcg ccaacgccga
      361 ccacccaagc cgatcgtcgg ggcgcgttgt tacggcttga ttgtcggtcc tggactgggc
      421 gagcctctcg ggaagaatga gtgggaaact ctgttcgccg ttgttggacc tggccgcctc
      481 tgcgattctc ctaaccggct gcgcggttgg gccaaaggct ccgctggccc gtcagacggg
      541 cgaatcggtc gtgttcatcg gcgaggaacc ggcggccctg gctcatttgc cggtgcgggg
      601 cgaaccgctc gaggtgcggg acacgtacct gccggcctcg aacagcgtcc gctacatcga
      661 gggccgcgat tttgtcgtgg attacgccgc ggggacgctc aagcggctgc cggattcgtg
      721 gctgccggat ttccgaacga acatgctgta tggccaggag aacttcgatc acacgcagtt
      781 tcccggctac ggcaacagcg ggtactttgc gtttgtggac tacacgcatg cggcaccggc
      841 gtcctggccc gtgcaggatg accagacgat gttcctgcgg tcgactgagg cgaagctgaa
      901 ggcgggcgag gcgctgaaga ttgttgcttt cggagacagc atcacggctg ggggcgatgc
      961 ctcgaagccc gatctgatct actggcagcg ctgggctgac gcactgcaac gcaagcatcc
     1021 cgattcgcgc atcaccgcgg tcaacggggc cactggcgga gattccaccg cgcaggggct
     1081 cgagcgactg cagaccaagg tgctcgacga gaagcccgat cttgtcctgc tcggcttcgg
     1141 catgaacgat cacaacgtga tgggcgtgcc gattccgcaa ttcgagtcga atctgatcca
     1201 gatgatcacc cgcattcgga ccgaggtggg ggcggaggtc atcctcttct cgactttccc
     1261 gcccaatccc aagtggaagt tcgggtcaca tcggatggag gaatacgccg cggccacgcg
     1321 tcgagtcgcg cgggagacgg cgtgcgccta tgcggatgtg ttcggcaact ggcaggcgct
     1381 ggcggggcgc aagaagccgg aggacctgct cggcaacaac atcaatcatc cgaacgactt
     1441 cggccacgga atctatttcc aggtgttcga agccctcggt ctgtgagcga ccggcgggca
     1501 agggttactg aggaatcggc gagatgcact ccaccgtcac ccccacccgc cctgcgcctg
     1561 cctgccctgt ctgccctgtc tgccgacagg caggcggcag gcaggggcac cctctcccat
     1621 cggatgggag agggccgggg tgaggggtct gtgtagcgta tccagccgac tcggcagtaa
     1681 gtcacgctga ttcctgaaga tgcgcccaac ctgcccgcgc tgtgcggtca gtcgttctgg
     1741 ctcgtcattt gctgtggaaa ggcgtctgct gggtcaagct acatgatcat gcgcacatat
     1801 tctgccgcgg cgctagggtt gattttgacc ctgacaccac cattcgtttt cggccaaccg
     1861 ccggcttacg gcctcttgcg cgaggtttgg gagggcatcc cgagcgagag actttccatg
     1921 ctgaccgact cgcccgatta tccggatcgt ccgaccagca ccaattatgt caccgatttg
     1981 ttcgaggcgc cgacggatgt tctggacaac tacggccagc gcatgcacgg gtatgtcgtc
     2041 gctccgcaga ccgggaacta caccttttgg atcgccagcg acgacaatgg cgctctgtat
     2101 ctgagcaccg acgagaatcc cgccaatgcg cgcgtgattg cgaccgtcaa ctcttggaca
     2161 agcgcgcgcg gctgggattg ggaggccaac cagagatcgg agcccatcgc actcatcgga
     2221 ggcaggattt actacatcgc cgccctcatg aaggaggggg gtggcggcga caacctggcc
     2281 gtgcgctggc tgatgcccga cggcacggat caggcgccga tggtcgcgac caacctgctg
     2341 ccttatggca tcagcttcgc cgcgccggtg atcgccgaac acccggcgaa cacgagtgcg
     2401 atcgaaggcg gcgccgcgcg ctttaccgtc aagaccgcga gcgtcggcgt ctatagctat
     2461 caatggcagc gaaacagcat cgccctgatc ggcgccaccg cggcggagct ggtttgcgac
     2521 cctgtgacac tggccgacca tggggcgcgg ttccgttgtg tggtcagcaa caagctcggg
     2581 accgccacca gcaacgaagg catcctgact gtcagccccg acaccacgcc cccagcgctc
     2641 gttcttgccc gtaacattgg gctgacgcag gtcgaggtga cgttcagcga gcgcgtttcg
     2701 gctccgtccg caaccaccgc cgcgaactac aaaatcgacg ctctcggtat cagcagcgcg
     2761 gcgttcggcc cgagttcgaa cgtgatctac ctgacgacat cgccgatgat cctgggcgcg
     2821 acgcacacgc tgaccgtcaa caatgtccag gacgccgcag ccacgcccaa tgccgtctcg
     2881 cccaacgcct cgattcagtt cgttgtgacg gatttcgcga cccaggacgt gggcaacccg
     2941 ccgctgatcg gagacgctgg atacgtcagc ggcggcgtcg atgtgcgggg aagcggcgac
     3001 atcggcggga cggcggacgc gctgcaattc gcctggcaga agcgcacagg cgcctttgat
     3061 gtgcgcgcgc gggtggtccg attcgactcg accgatccgt ttgccaaggc gggtttgatg
     3121 gtgcgcgagg atttgagcgc ggggagccgg tttgtggcgg cgctggccac acctgcggcg
     3181 gtgggttgcg tcttcatggc tcgaagcgcg ccgggggccg cggcgagtcg atcgggctat
     3241 tgcgccgcga actacccgga gacctggctc cgcctgaagc ggtcggcgaa cctcttcacc
     3301 gcgtatgccg gctacgacgg cctgtcctgg gtcgagcttg gctcggcgca actggcgctg
     3361 cccgaaacgg tctacttcgg tctggcctcc gccagccgca acacggccgc cctggcaacc
     3421 gccagtttcc gcgacatcgg cgccgtgtcc caagcgacgt tgatgacctc ggcgccgcgc
     3481 ggggaacggc tcgggccgag cagccgccag acgccgctcg tgatcagtga gatcatgtac
     3541 cggccgctgc ggcgtccgga cggcaagacg ggcgagttca tcgaattgta caacgccgat
     3601 ctcatcgccg ccgatctgac gggacaccgt ctgggcggca gcatccagtt cgcttttccg
     3661 gacggattcg tgctggccgc gggcggtttt gccgtcatcg cccggaatcc ggtcgatttc
     3721 gcggcggtgt acgggattgc ggacgcgctg gggccattca taggcggcga tggcttgccc
     3781 aacgacggcg gcaccgtgcg cctcgagaac ccagaaggcg ccaccctgct cgaagtgaat
     3841 tacgagtccg atttcccctg gccggtggcg gcggacggcg ctgggcattc gctggtgctg
     3901 gtccggccct cgtatggcga ggacgatccg cgcgcgtggg cggccagtga cgtgatcggc
     3961 ggttcgccgg gacaaatgga ggccgtgcgg gccaacccgc tggccggcgt cctgatcaac
     4021 gaattcctcg tccgtcccga tgctgcgcaa cgcgggttca tcgagttgta caaccacagc
     4081 aacaacgggg cggatgtggg cggatgcatt ctgacggatg atgcgcagac cgctcgattc
     4141 cacattgcgg aaggcacggt gattccgcct cgcgggttcc tcgcgtttta cgaagaccag
     4201 atccggttcg ggctcggcgg tgcgggcggg cggcttttcc tggtcaactc gaatgccact
     4261 cgagtgctcg atgccgtccg gtacggcgcc caggaacgcg gcatggccat gggacggtat
     4321 ccggatggcg cgccgggtgt gcgccgactt atcgagccgt cgccggacaa cgagaaccgg
     4381 tcgcaggcca tcgacgtcat tgcgatcaac gagatcatgt acaaccccat ttcgggcgac
     4441 gacgccgatg agttcatcga gttacacaat cgaggggcgc agcgcgtaga cctaggtggc
     4501 tggaggttca cagatggtgt cgattttcag ttcccggcgg gaacgatcat cgagggggga
     4561 ggttatcttg tggttgcgaa gagccggctg cgtctattgg ccagccatcc cggactcgat
     4621 cctgccctgg tcttcggcga ttacggcggg gcgctggcca acggcggcga acgggttgcg
     4681 ttggccaaac ccgatctcct ggtcctcacc aacgcgttcg gcctgccgga gacgaatcgc
     4741 atcttgatcg atgtcgatga ggtcagatac ggcgtcgggg ggcggtgggg gcagtggagc
     4801 gacggtctgg gcagcagtct cgagttgacc gatcctcgaa gcgatcactc gagtccgtcg
     4861 aactggg
//
LOCUS       DAOQRO010000278         4827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18470_length_4827_cov_4.190486, whole genome shotgun sequence.
ACCESSION   DAOQRO010000278 DAOQRO010000000
VERSION     DAOQRO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18470_length_4827_cov_4.190486"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PKK20_10230"
     CDS             <1..1346
                     /locus_tag="PKK20_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNV00304.1"
                     /translation="EIGTVTDTGRLVVRWQGQVVADIPTRQIADESPVYEREVREPRY
                     LESVRAFTLDGIPDTADPAPQLISLLSWSTIASKNWTYRQYDHMVRDGSVVCPGSDAA
                     VIRIKADSVPPPPAGAGGAGPAPAPSVPEKLVALTVDCNGTYVFLDPYEGGKIAVAEA
                     ARNLACSGARPVGVTDNLNAANPRDPGLFWQLRESVRGLGEACRIFQIPITGGNCSLY
                     NRVPDGPVDPTPTIAMVGLIDQPEHIATQWFKDEDDAVILLGEPCDAADPLQGLGGSA
                     FLQVVHGQKSGRPPRCDLRAAQTLHDALRGLICSGSVKSAHDCSEGGLAVCLAECCVS
                     QQVVRETPRLMGASIDLSSLAAASGAVEGSTEAPPIRLDALLFGETQNRVVITTHRLH
                     VAKVLERARLLGVAALHLGWVGGDRLTVRTARCEWSWALSELHDAWWNSIGRAMA"
     gene            1574..>1954
                     /locus_tag="PKK20_10235"
     CDS             1574..>1954
                     /locus_tag="PKK20_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00305.1"
                     /translation="MRAGFTLIELLVVIAIIAILAGMLLPALARAKAKAHNINCLSNL
                     KQLTLAWTMYAGDHNDTLVLNTLADTNAWIGGGVSAMPGATNLLDIINGKLYPYNSSP
                     EIYRCPVDTKMPPDIAAALRGKRRV"
     assembly_gap    1955..1964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1965..2409
                     /locus_tag="PKK20_10240"
     CDS             <1965..2409
                     /locus_tag="PKK20_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00306.1"
                     /translation="LSQVQRDAGWTHRVRSYSMCGRMGGHTDQNWVLNPSGPILYPPR
                     KKMTDITVPSPSEAFVFIEESYLTIDDGYFATKAPGVLIWQNSPTIRHGKAGELSFAD
                     GHAEVWKWRFLTTDQDLDTGVVVRGINSTPDLVRLQAAVARVGGG"
     gene            complement(2424..3770)
                     /gene="ffh"
                     /locus_tag="PKK20_10245"
     CDS             complement(2424..3770)
                     /gene="ffh"
                     /locus_tag="PKK20_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNV00307.1"
                     /translation="MFDSLTSKLQDVFRDLRGLGRISETNVSESLREVRLALLDADVN
                     FKVARDFIERVREKAIGQEVIRSIQPGQQIIKIIHDELVALLGSENASLDLGGSPACI
                     LMVGLHGSGKTTSSGKLARMLEKQGRAPLLIAADVYRPAAMDQLATLGEQLGVPVFVQ
                     KEEKDVVRIARAALVFAVANQRNVLIFDTAGRLQIDEPLVQELVRLRDEVHPREILLV
                     LDAATGQEAVNVATHFDQALGMTGAVLTKLDGDARGGAALSFKAVVGKPIKLMGVGEK
                     LEDFEPFHPERMAQRILGMGDVVSLVEKAAEAVDVEEAMRIEERMRKGQFTLQDFLDQ
                     LRQMKKLGPIETIVGLLPGGGDMLKGADLAKGEREFRRMEGIICSMTPQERRSPNILN
                     AKRRVRIANGSGVKVSEVNNLLNRFNQMQQMMKKMGKFKKMMARMGGAPLPGMFPR"
     gene            4160..>4827
                     /locus_tag="PKK20_10250"
     CDS             4160..>4827
                     /locus_tag="PKK20_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV00308.1"
                     /translation="MEAAGGIAASQPQPQLIASPAPGWPQWRGPRRDGICDEVGLLEE
                     WPADGPRLVWQTNGLGRGYSAPVIVGDRLFLAGDVGDQLRLFALDLTGRQLWQKPNGR
                     SWPGPYPGARASAAWHEGRLFHLNAHGRVAAFEAETGREIWAVDLFERYGGKNLTWAT
                     SECLLVDGSNVLVTAGGAEALMVALDVRSGETVWKAEPLRLGPSSDPRHQRVEEPAGQ
                     VDNA"
BASE COUNT          832 a         1499 c         1561 g          925 t
ORIGIN      
        1 cggagatcgg gacggtcacc gacaccgggc gcttggtggt tcggtggcag gggcaagtcg
       61 tggccgacat cccgacgcga caaattgcgg acgaatcgcc cgtgtacgaa cgcgaggttc
      121 gggagccccg ctacctcgag tccgtccgcg cattcacgct cgatggcatc ccggacaccg
      181 ctgatccggc gccgcaactg atctcgctgc tgtcgtggtc gaccatcgcg tccaagaact
      241 ggacctatcg ccagtacgat cacatggtcc gcgacggctc ggtcgtttgc ccgggtagcg
      301 acgccgcggt gatccggatc aaggccgact cggtcccgcc tccgcccgcc ggggcgggcg
      361 gcgcggggcc ggctccggcg ccgtccgtgc ctgagaagct cgtcgccctt accgtcgact
      421 gcaatggaac ctatgtcttc ctcgatccgt acgaaggcgg gaagatcgcc gtggccgagg
      481 cggcgcgcaa cctggcgtgc tcgggggcgc gtccggtggg agtgaccgac aatctcaatg
      541 cggccaatcc gcgcgatccc gggttgttct ggcaactgcg cgagagcgtc cgcggattgg
      601 gcgaggcatg tcgcatcttc caaatcccga tcaccggcgg caactgcagc ctgtacaacc
      661 gggtccccga cggacctgtg gaccccaccc cgaccatcgc gatggtgggg ctgatcgatc
      721 agccggagca catcgccacg caatggttca aagacgagga cgacgcggtc attttgttgg
      781 gagagccgtg cgatgccgcc gatccgcttc aaggcttggg tggcagcgct tttctccagg
      841 ttgtgcacgg ccagaagagt ggacgtccgc cccgctgtga tctccgggcg gcccaaacac
      901 tgcacgacgc gttgcgcggc ctgatttgct ccgggtcggt caagagcgcc cacgactgca
      961 gcgagggcgg gctggctgtt tgcctggccg aatgctgcgt cagccaacag gtcgttcgcg
     1021 aaaccccgcg tctcatggga gcatccattg atctgtcgtc ccttgcagcg gccagcggag
     1081 cggtggaagg ctcgacagag gcgcccccga ttcgtctcga tgccctgctt ttcggcgaga
     1141 cgcagaaccg cgtggtcatc accacgcatc gattgcacgt cgccaaggtc ctcgaacggg
     1201 cgcggttgct tggcgtggcc gcattgcatc tgggatgggt ggggggggac cggctgacgg
     1261 ttcggaccgc tcgatgcgag tggagttggg ctctgagcga actgcacgat gcatggtgga
     1321 actcgatcgg gcgcgcgatg gcttagggtc tgtgcactga cggggtgtaa tatgcgggtt
     1381 agcccggcat cgtctcaaac cgatgagggg cgtgaaagac agaagcgctc aacgctcaat
     1441 gctcaacgat caaccagggt gcttgacgac agcggacggg agtcttacgg tggcctcaaa
     1501 actgctcaaa tgaacctgta gacgagacat tattatgcgc gcattaacct caggtttgtc
     1561 tagccgctct cgaatgcggg ccggtttcac tttgatcgag cttttggtgg tgattgccat
     1621 catcgcgatt ctggcgggga tgttgttgcc ggcgctggcc cgagccaaag ccaaggcgca
     1681 caacatcaat tgcctgagca atctcaagca gttgacactc gcctggacca tgtacgccgg
     1741 cgatcacaac gacacattgg tcctcaacac tctggccgac actaatgcct ggatcggcgg
     1801 cggagtgagt gccatgcccg gcgcgaccaa cttgctcgat atcatcaacg gcaagctcta
     1861 tccctacaac agttctcccg agatttatcg gtgtcctgtc gataccaaaa tgccgcccga
     1921 catcgcggcg gccctgcgcg gaaaacgccg ggtcnnnnnn nnnngctcag tcaggtgcaa
     1981 cgcgacgctg gttggacgca tcgggtccgc agctactcga tgtgcggtcg aatgggaggt
     2041 cacacagatc agaattgggt tctcaacccg tccggcccca tcctctatcc cccgcgcaag
     2101 aagatgaccg acatcacggt gccgtcaccc tccgaggcgt ttgtctttat cgaggagagc
     2161 tacctgacca tcgacgatgg gtactttgcg accaaagcgc ccggtgttct gatttggcag
     2221 aactcgccga cgatccggca cggcaaggcc ggcgagttgt cgtttgccga tgggcatgcc
     2281 gaggtctgga agtggcgttt cctgaccacc gaccaagacc tcgatacagg cgtagtggtg
     2341 cgcggcatca actcgacccc cgaccttgtg cggttgcagg ccgctgtggc tcgagtcggc
     2401 ggaggctaac cgggttcggg cttctaacgc gggaacattc cgggcaaggg cgcgccgccc
     2461 atgcgcgcca tcatcttttt gaacttgccc attttcttca tcatctgctg catctgattg
     2521 aaccggttga gcaggttgtt cacctcggac accttgacgc cgctgccgtt ggcgatgcgc
     2581 acgcggcgtt tggcgttgag gatgttcgga ctgcgccgtt cctgcggtgt catgctgcag
     2641 atgatcccct ccatgcgccg gaactcgcgc tcgcccttgg ccaggtccgc gcccttgagc
     2701 atgtcgccac cgccgggcaa caggccgaca atagtctcga tcggccccag tttcttcatc
     2761 tgtcggagtt gatcgaggaa atcctggaga gtgaattggc ccttgcgcat ccgttcctcg
     2821 atgcgcatgg cttcttcgac atcgaccgcc tcggccgctt tctcgaccag gctgacgaca
     2881 tcacccatgc ccaggatgcg ttgggccatg cgctcgggat ggaacggctc aaaatcctcg
     2941 agtttctccc ccacgcccat gagcttgatc ggctttccga cgacggcctt gaagctcagc
     3001 gccgcgccgc cccgggcgtc gccgtcgagc ttcgtcagaa cggcgcccgt catgccaagg
     3061 gcctggtcga aatgtgtggc cacattgacg gcttcctgtc ccgtggcggc gtccaggacc
     3121 agcagaatct cgcgcggatg cacctcgtcg cgcagccgga ccagttcctg gaccagcggt
     3181 tcatcgatct ggaggcgtcc ggcggtgtcg aagatcagca cattacgctg attggccacg
     3241 gcgaaaacaa gcgccgcccg cgcaatgcgc accacgtcct tttcctcttt ctgtacgaac
     3301 accggcacgc ccagttgctc gccgagcgtg gccagttggt ccatggccgc ggggcggtag
     3361 acgtcggcgg caatcagcag cggcgcgcgg ccctgctttt cgagcatccg cgcaagcttg
     3421 ccgctcgatg tcgttttgcc cgatccgtgg aggcccacca tcagaatgca ggcgggcgaa
     3481 ccgcccagat cgaggctcgc gttttcagac cccagcagcg ccaccagttc gtcgtggata
     3541 atcttgatga tctgttggcc cggctgaatg ctccgaatca cttcctgccc gatcgccttc
     3601 tccctgaccc gctcgatgaa gtcgcgggcc actttgaaat tcacatcggc gtccaacagc
     3661 gccaagcgca cctcgcgcag cgattcggac acgttcgtct ccgaaatgcg gcccaatccg
     3721 cgcaggtcgc ggaagacatc ttgcagcttg ctcgtcagcg aatcgaacat gggccgcagt
     3781 ttagccggaa aggctgccga aatccagggc caaccgttca ggccggagat gggagatcgg
     3841 tgctgggaga tggggggttg accaccgaga cgcatagcac acagagaagg ccggttgaag
     3901 ctgagccgga tattggcctt ctccgcgcgc tctgtgcctc cgtggttcaa atcgggacgg
     3961 tgacagtcat cgaggggttg cgccgcagcc agtccgcgct aggcttcggc cacagatgat
     4021 ctcgcgagag cttcttttag ttcgcgtctt ggcgtcttgg cgtgaagtcg tccgcgccag
     4081 ttctgcagtc gcgcgaccca ggcgacgaat ggcctggttg gcgatggctt ttgcgcacgg
     4141 tctcagtagc tccgtgtgcg tggaggccgc ggggggcatc gccgcttcgc agccgcagcc
     4201 gcagttgatc gcttcgcctg caccgggttg gccgcagtgg cgcgggccgc gacgcgacgg
     4261 catctgcgac gaggtcggat tgctcgagga atggcctgcg gacggaccgc gattggtctg
     4321 gcagaccaac ggcctgggcc ggggatactc ggccccggtg attgtcggcg atcggctctt
     4381 tctcgcgggc gatgtcggcg accaactgcg cctcttcgcg cttgacctga ccggtcgaca
     4441 gctttggcag aaaccgaatg gacgttcgtg gcctgggcct tacccggggg cgcgggcgag
     4501 cgcggcatgg cacgaggggc ggctgtttca tttgaacgcg cacgggcggg tggctgcatt
     4561 cgaggccgag acaggtcgcg agatctgggc ggtcgattta ttcgagcggt acgggggcaa
     4621 gaacctgacc tgggcgacga gcgagtgtct gctggtggac ggctcgaatg tgctggtgac
     4681 ggcaggcggc gccgaagccc tcatggtggc gctcgatgtg cgcagcggcg agacggtttg
     4741 gaaggccgaa ccgctccggc ttggtccttc gtcggaccct cggcaccagc gcgtggagga
     4801 gccggcaggc caggtcgaca atgccag
//
LOCUS       DAOQRO010000279         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18770_length_4773_cov_4.915642, whole genome shotgun sequence.
ACCESSION   DAOQRO010000279 DAOQRO010000000
VERSION     DAOQRO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18770_length_4773_cov_4.915642"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKK20_10255"
     CDS             <1..268
                     /locus_tag="PKK20_10255"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNV00309.1"
                     /translation="LEDARFLAEAGAFAVVLELVWPAVALEVTRAVAIPTIGIGSGPD
                     CDGQILVVNDLVGTFPWFTPRFVTPRVRAFDQMRAAIEDWSRSV"
     gene            complement(592..1605)
                     /locus_tag="PKK20_10260"
     CDS             complement(592..1605)
                     /locus_tag="PKK20_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV00310.1"
                     /translation="MVRLVDIARQAGVSVMTVSRVLRDAPNVSTATRTRVHALAEQMG
                     YVPNAMAQSLRTRSTRLIAYVVPSVADPMATRILLALSEQAHDLGHDLLVSQSLNRLD
                     REETCIRRALARRVEGFFLQPVYRLDPSAPAYDEVRRHGAKVVVLGPKPPFCEAFVNV
                     EADEALAAGEATAHLIELGHRRIAFLSGPPTSPAAQNRFEGYRRALRKAGLEVDDGLI
                     FHAGETMEDGMKAAAQMLGESAAATAVQAVNDQVAMGAAAFFFDRGLRIPQDISVVGC
                     GNIPFSECFRVPLTTVRQPKYQLGLAAVGMMQALLRGESVSSRLLPGTLVVRASTGPP
                     RAG"
     gene            complement(1687..2571)
                     /locus_tag="PKK20_10265"
     CDS             complement(1687..2571)
                     /locus_tag="PKK20_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNV00311.1"
                     /translation="MKRLPDSIRRVGLVGNPEKVATRSVARKARALIAAAGRDVLVDA
                     GTAEFVGLSDARFESVTALAGECDLLIVLGGDGTILRVARQAAGSGTPILGINLGRLG
                     FLTASSSANLGRTLEAVWSGQCHVESRPLIEATGRGRHGRIHELALNDFVVSHGMVPR
                     LIELEVKVDREVLTSYRGDGLIVSSPTGSTAYSLAAGGAVVSPQADVFALTPICPHTL
                     SNRSVIVSLSSTVEVRLLSERVDTFLAADGQTRAELRVGDWLKVRRSRSTTHLVRLPE
                     SSFFGTLRQKLNWSGSSV"
     gene            complement(2555..3301)
                     /locus_tag="PKK20_10270"
     CDS             complement(2555..3301)
                     /locus_tag="PKK20_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNV00312.1"
                     /translation="MCERLDAALVRRNLSPSRDKAKRMVLAGQVRVDGQVARKPSEPV
                     RDSAVIAIDAPEKYVSRGGLKLEHALESFGIDARGATALDVGASTGGFTDCLLQHGAA
                     RVFAVDVGRGQLAWKLRQDVRVHAIEGLNARHLAAGCFPAPFAPVDIAVIDCSFISLR
                     LVLPPTAALVRPRGCLVALVKPQFEAGRAEASRGGGVITDPAVHRRVLGELEAFVGGF
                     GGLRWRAVTESPILGPAGNKEFLALIEKTT"
     gene            complement(3384..3833)
                     /locus_tag="PKK20_10275"
     CDS             complement(3384..3833)
                     /locus_tag="PKK20_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNV00313.1"
                     /translation="MPANAKALTEEFLRLLGFPCTVESSQLDDGMLIDIQCDDAGRLI
                     GRQGQTLSEMQYLINRMIFQQDKEHPKITLDVGGYRSQAREALVQKAMEAAEKVRRWG
                     DIVELEPMNAFDRRIVHNALKDDPGVETHSVEVDGTVKKAILLRPKH"
     gene            complement(3903..>4773)
                     /locus_tag="PKK20_10280"
     CDS             complement(3903..>4773)
                     /locus_tag="PKK20_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HNV00314.1"
                     /translation="DSRIKAKPVGYQAAFVYAPLAIPPGQALERRLEVFAGPKEYNTL
                     AKLGRNVDLVMGFGGFFGWFAKLLLTSMNGLHSLGLPYGATIIAITVIIKILFWPLTQ
                     ASTRSMKRMQALQPQMKALQEKYKSDPKKMNQKLMEFMKENKVSPLGGCLPLMLQIPV
                     FFGFFQMLRSAIELRGARFLWACDLSQPDTVALVFGFPINPLPLIMGATQIWQMRLTP
                     PSPGVDPVQQKMMQYMPLIFVFMLYNFSAGLALYWTVQNLLSILQMKLTKTLTPAQSA
                     APLKPPTRPAKGK"
BASE COUNT          864 a         1593 c         1526 g          790 t
ORIGIN      
        1 gctcgaagac gcgcgcttcc tggccgaggc gggggctttt gccgtggtgc tcgaactggt
       61 ctggccggcc gttgccctcg aagttacccg cgcggtagcc atcccgacca ttggcatcgg
      121 atccggaccc gactgcgacg gccaaatcct ggtcgtcaat gacctggtcg ggacatttcc
      181 ctggttcacc ccgcggttcg tgacgccgcg cgtccgggcc ttcgaccaga tgcgcgcggc
      241 catcgaggac tggagccgct cggtgtaact cgagcccttc acggcgcgca acacttgtgc
      301 acaaaactgc ttgccactcg gccctgccaa gactgtgggc ctctgcacgg aatccgagca
      361 tccgcatggc gcagcccgtg ccgtggcatt gccaaatcgg cggacttcct cgttggggcc
      421 gctcctggaa ttggggctat gattcacgca atgacgcgaa gacgcctgga caactgcccc
      481 accctgtccg gccttggcgg ctttgcgtct tggcgtgaga tcatggcctg tgcgaagcag
      541 gcgaccgcaa cccaccgatc cgcgttgccc tgaatttgtg tcatgcgcgg gctaaccggc
      601 tcggggaggt ccggtgctgg cgcggacaac gagcgtgccg ggcagcaacc gggaactgac
      661 cgattcgccg cggagcagcg cctgcatcat gccaacggcg gccaaaccga gttggtattt
      721 gggctgccgc accgtcgtga gcggcacgcg aaagcactcg ctgaacggga tgttgccgca
      781 gcccacgacc gaaatatcct gcgggattct caggccgcga tcgaagaaga acgcggcggc
      841 tcccatggcg acctggtcgt tgaccgcctg aaccgccgtg gcggcggcgc tctcgccgag
      901 catctgcgcg gccgccttca tcccgtcttc catcgtttcg ccggcatgaa agatgagacc
      961 gtcatcgacc tcgagcccgg ccttgcgcag ggcgcgacga taaccctcga agcggttctg
     1021 agcggcgggc gacgtgggcg ggcctgacag gaatgcgatg cggcgatggc caagctcgat
     1081 caaatgggca gtggcctcgc cggccgccag ggcctcgtcg gcctcgacgt tgacgaacgc
     1141 ctcgcagaac ggcggtttag gccccaagac aaccaccttc gccccgtgcc gccgcacctc
     1201 gtcgtacgcg ggggcggagg gatcgagccg atagaccggc tgaaggaaga agccctcgac
     1261 acgacgggcg agcgcgcggc ggatgcaggt ctcctcccgg tcgagccgat tgagcgactg
     1321 gcttacgagc aagtcgtgtc caagatcatg cgcctgctcg ctgagggcca gcagaatgcg
     1381 cgtggccatc ggatcggcga cgcttgggac gacataggcg atcagccgcg tgctgcgggt
     1441 gcgcaaactc tgggccatgg cattgggcac atagcccatc tgctccgcca gggcgtgcac
     1501 acgcgtccgg gtggccgtag agacgttcgg cgcgtcccgc aatacgcggg agaccgtcat
     1561 tacggacaca ccggcctgcc tcgcgatgtc aaccagacgt accatgtcat tgcggcttgc
     1621 ggaggcagac gcgtcgcgcg accgtcgaac ccgggcaagg ccgggcctga tcgccggctg
     1681 ctctcctcag acgctggacc cgctccagtt cagtttctgg cgcagcgtcc caaaaaacga
     1741 gctctccggc aagcgcacca agtgcgtcgt gctgcggctg cgccgcacct tgagccagtc
     1801 gccgacccgc aactcggcgc gggtctggcc gtcagcggcc aggaacgtgt ccacccgttc
     1861 gctaagcagg cggacctcga cggtcgaact caggctcacg atcaccgacc gattcgagag
     1921 tgtatgcggg cagatgggcg tgagggcgaa cacatcggcc tggggactca ccacggcgcc
     1981 gccggcggcg agcgagtacg ccgtcgaccc ggtcggcgaa ctgacgatca agccatcgcc
     2041 tcggtagctg gtcagcactt cacggtcgac cttcacctcg agttcgatca ggcgcgggac
     2101 catgccgtgg ctgacgacaa agtcgttgag cgccagctcg tgaatccgcc cgtggcgtcc
     2161 ccgacccgtg gcctcgatca ggggacgcga ctcgacgtgg cactgccccg accacaccgc
     2221 ctcgagggtg cggcccaggt tggcggacga actggcggtg agaaagccca gacggcccag
     2281 gttgatcccc agaatcggcg tgccggagcc ggcggcctgc cgggcgacac ggagaatcgt
     2341 gccgtcccct ccaagcacaa tcagcagatc gcactccccg gccagcgccg tgaccgactc
     2401 gaaacgcgcg tcggaaagcc cgacaaactc ggccgtgccg gcatcgacga gcacgtcccg
     2461 gccggcggcg gcaatcagcg ccctcgcctt gcgcgccacg ctgcgggtgg cgactttctc
     2521 cggattcccg accaggccca cccggcgaat cgaatcaggt agtcttttca atcaacgcca
     2581 agaactcttt gttgcccgcc ggccccagaa taggtgattc cgtcaccgca cgccagcgca
     2641 aaccgccgaa gccgccgacg aaggcctcga gctcgccgag gacacgccga tgcaccgccg
     2701 gatcggtgat gacccccccg ccccgactcg cctcggcccg accggcctcg aactgcggct
     2761 tcacgagagc cacaaggcag ccccgggggc ggacgagcgc cgccgtgggc gggaggacga
     2821 gccgcaacga gatgaaggaa cagtcaatca ccgcgatgtc caccggggcg aacggcgccg
     2881 gaaaacaacc cgccgcaaga tggcgcgcat tcagaccctc gatggcgtgc acccggacgt
     2941 cctgacggag cttccaggcc agttgaccgc gcccgacatc gacggcgaat acccgcgcgg
     3001 cgccgtgctg gagaagacaa tcggtgaacc cgcccgtgga agccccgaca tcgagggcgg
     3061 tggcgccgcg cgcgtcgatg ccaaacgact cgagcgcatg ctcgagcttc aatccgccgc
     3121 ggctcacata tttctcgggg gcgtcgatcg cgatgacggc cgagtcgcgg accggctcgc
     3181 tgggctttcg ggccacctgg ccatccaccc gcacctggcc cgctagaacc atgcgcttgg
     3241 ccttgtccct gctcgggctg aggttgcgcc gcaccagagc ggcgtcgagg cgttcacaca
     3301 cgagccgcgg ctcagaccga cgaggcgtgt cctcagttcc cttgcgctga ggcctggcca
     3361 tggcagcggc ggccgacgcc tcactaatgt ttcggccgca ggagaatggc cttcttgacg
     3421 gtgccgtcca cctcgacgct gtgcgtttcg acgccgggat cgtccttgag cgcgttgtgg
     3481 acaatgcgcc ggtcgaacgc attcatcggc tcgagttcga cgatgtcgcc ccagcgccgg
     3541 accttttcgg cggcttccat cgccttctgg accagcgcct cgcgcgcctg ggacctatac
     3601 ccgccgacat cgagagtgat cttgggatgc tccttgtcct gctggaagat catccggttg
     3661 atcaaatact gcatctcgct gagggtctgg ccctggcgtc cgatcaaccg cccggcgtcg
     3721 tcgcattgga tgtcgataag cattccatcg tcgagctggc tcgattcaac ggtgcatgga
     3781 aaccccagga gacgaaggaa ctcctcggtg agcgctttgg cattggcagg cataaatgtg
     3841 gcgaggtttg agtgttcaga aagcgtgtcg aaaccaccca tgggccgcaa agcgtatccg
     3901 catcacttgc ctttggccgg ccgggtcggc ggtttgagcg gcgccgcgga ctgggccggc
     3961 gtcaaggtct tggtgagttt catctgcagg atgcttagca ggttctggac ggtccagtaa
     4021 agcgccaacc cggcggagaa gttgtacagc atgaacacaa aaatcagcgg catgtactgc
     4081 atcatcttct gctggaccgg atccacaccc ggcgatggcg gggtcagccg catctgccaa
     4141 atctgggtcg cccccatgat cagcggcagc gggttgatgg gaaagccgaa caccaatgcc
     4201 accgtgtccg gttgcgagag gtcgcaggcc cagagaaagc gcgccccgcg caactcgatc
     4261 gcgctgcgca gcatctggaa gaagccgaaa aacaccggaa tctgaagcat gagcggcagg
     4321 cagcccccca gcgggctgac cttgttttcc ttcatgaatt ccatcagctt ctgattcatc
     4381 ttcttgggat cggacttgta cttttcctgg agcgccttca tctgcggctg cagggcctgc
     4441 atgcgcttca tcgaccgtgt gctcgcctgg gtcaacggcc agaagagaat cttgatgatg
     4501 acggtgatgg cgatgatcgt cgcgccatac ggcagcccca gactgtgcag tccgttcatc
     4561 gacgtgagca gcagcttggc aaaccacccg aagaacccgc caaagcccat gaccagatcg
     4621 acgttgcgcc ccagcttcgc cagggtgttg tactccttcg gcccggcgaa gacctccagc
     4681 cggcgctcga gcgcctggcc gggaggaatt gccagaggcg catagacaaa cgcggcttgg
     4741 tatccgaccg gcttggcctt gatcctcgag tcg
//
LOCUS       DAOQRO010000280         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18821_length_4763_cov_3.492566, whole genome shotgun sequence.
ACCESSION   DAOQRO010000280 DAOQRO010000000
VERSION     DAOQRO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18821_length_4763_cov_3.492566"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3411)
                     /locus_tag="PKK20_10285"
     CDS             complement(<1..3411)
                     /locus_tag="PKK20_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV00315.1"
                     /translation="MTSRTRSFFVLACLLGAFGFWQLAHRRAALEKIAPSDRLTRTAQ
                     TASESAEPTPDVRLLTNLTPLPAAVRPMDQSAAAIAPDSDAVEPTVDPQFPRRVRNTS
                     KPIRELAHSDSAILLRNALIDTASDVPLSLPPHLKTGEEPGSYVIQAKGPITEAFRAA
                     LADQGADIVSYIPNNAYLVRASASAAHALRVSGSARAVIPFEPYFKIDPSLLELAAEQ
                     QPLPPRARLRVTLLPESGAGARHALEAIGANILAEARSPFGPQFVVEPPPGEVAALAC
                     LPEVQLVEPWVARLPMNDLTGVRMGVLWNTVDGGASTNLTLPLTGSNICVNVNDSGVK
                     TNHPDLMLGGQHRIYGDTNAASLVDTNGHGTHVAGTIIGLGTSVTAGTNVYGSLSNAV
                     FRGIAPAASLFVLPIDLDGGPTISDAYLQETAANTRYGTNHSLTNILISNNSWGYPGR
                     FEYDSSAASYDAATRDALPYTPGPQALLFVFAAGNSGAGNDVGTGGEPGTITSPGTAK
                     NVLTVGATELNRFVYITNIVTNIIDVIDTNGEPITVTNIEAGQYTFSETDSDSQVAPF
                     SSRGNVGIGFESEVGRFKPDVVAPGTFVYSTWIDDWPTNGLVTNRVVQTYAQQIVFPG
                     ETNTYVYTVASNVVYLEIEALPNERTILPFSPLTIRARLNGADVTGQTNVVVTPLSEG
                     TLTYTITTDEAQAEAVDFDLRVTTLVENSLGIYVWPYTQSYTNSTPGARYRFNSGTSS
                     SAAAVSGMLALLQELFEQHLETTDSPALLKALIINGARSLGPEYSYGVKASYNYQGWG
                     LANLTNTLPLAITNALSDTSNWPVQWVDQSPSNALATGQTHEYQIALPTNASPSTLRV
                     TLVWTDPPGNPAAASKLVNDLDLEVQQGTNQIVYGNDFEPDSDFNTIHPVGTNGVPDA
                     PRDTINNVENVFLSLPSSTNYTVRVIGRRVNVNAVTAHTNNIVQDYALVVSIDSTNRL
                     ELRPLPTNSTAFTMPTPDVLTNGLPRLHDRVGANSPLLEYPLGTRLQWHFYVFTNAPT
                     PYDWYTNFGTNVAIALHTPLNLSVARNVEPDIDLYVSRDRRLLDLDPQAIAAAERSIQ
                     RGGSSETLMYSNAVVNPSEVFYIGVKSEDQMAAEYSIVALSTMEPFTTI"
     gene            complement(3451..>4763)
                     /locus_tag="PKK20_10290"
     CDS             complement(3451..>4763)
                     /locus_tag="PKK20_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00316.1"
                     /translation="LGNITTSAQQWRSWSAVSAEGRDKERSIDRLRLFCGLTPLTYTS
                     QRERDRLAQEIAGKLAIQAGYSPTRKVYQEITWQANDPLVHYTLGDLLDPFNPPNDPN
                     RTNAVRFAIPPQITLTNSNLGLLNQRYRPWGGNPNESADVLARDIRVKDPLIERSDDW
                     AFPTNKFPNVGWIGRVHRGTPWQTVYLKADIADTNTTTQTSWYRWAGSQGTHPTNDWT
                     LADLFTTAPTDNAQRGLLSVNQDNLAAWAAVLSGVSVLLPIDDTGALDELFLDQVSSN
                     LFEPSATNLSTLQKIVDGINRTRAQETNLLIGVHAPYFDSLGRILATPELTFASPPAT
                     PGSTGMLPRKLPVWPRDEVLERIPQQILSLLKADEPRFVVYAFGQTLKEAPNSLYFGE
                     GIFNRMCTNYQVKSECVTRSVVRLDGPLDTPRAVVENFNEVLSE"
BASE COUNT          772 a         1264 c         1797 g          930 t
ORIGIN      
        1 gatggtggtg aaaggctcca tcgtcgagag ggccacgatg ctgtattccg ccgccatttg
       61 atcctcggac ttgacgccga tgtagaacac ctccgacggg ttcaccaccg cgttggagta
      121 catgagtgtc tccgacgatc cgccgcgctg gatcgagcgc tcggcggcgg cgatcgcctg
      181 cggatcgagg tcgagcagac gccggtccct ggacacgtag aggtcgatgt ccggctcgac
      241 attgcgcgca accgagaggt tcagcggggt gtggagggcg atggcgacgt tggtgccgaa
      301 gttggtgtac caatcgtagg gcgtgggcgc attggtgaac acatagaagt gccactggag
      361 ccgagtgccc aacgggtatt cgaggagggg ggagttggcg cccacccggt cgtggagccg
      421 gggcaggccg ttggtcagca catcgggcgt aggcattgtg aacgcggtcg agttggtggg
      481 cagaggcctg agttcgaggc gattcgttga atcgatcgac accactagcg cgtagtcctg
      541 gacgatgttg ttggtgtggg cggtgacggc gttgacgttg acccggcgtc cgatcacgcg
      601 gacggtgtaa ttggtgctcg agggcaggct caagaagacg ttctcgacgt tgttgatggt
      661 atctctgggg gcgtcgggga cgccgttggt gccgacgggg tggatcgtat tgaagtcgga
      721 gtcgggttcg aagtcgttgc catagacgat ctggttggtg ccctgttgca cttcgagatc
      781 gaggtcgttg acgagtttgg atgccgccgc cggattgccg ggcgggtcgg tccagaccag
      841 tgtcacgcgc agcgtggatg gggaggcgtt ggtgggcaac gcgatctggt actcgtgcgt
      901 ctgcccggtc gcgagggcgt ttgaggggct ttgatcgacc cattggaccg gccagttcga
      961 ggtgtcggag agggcgttgg tgatggccag gggcagcgtg ttggtgaggt tggcgaggcc
     1021 ccagccctgg taattgtagg aggccttcac gccatagctg tactcgggtc ccagagaccg
     1081 cgccccattg atgatgaggg ccttgagcag ggccgggctg tcggtggtct cgagatgctg
     1141 ctcgaacaac tcctggagca gggccagcat gcctgaaacc gccgccgccg aggagctggt
     1201 gccggaattg aaacgatagc gcgcgcccgg cgtgctgttg gtgtaggact gcgtgtacgg
     1261 ccagacataa atgcccagcg aattctcgac cagcgtcgtc acacggaggt cgaaatccac
     1321 cgcctcggcc tgggcctcat cggtcgtgat ggtataggtg agcgtgcctt cggaaagcgg
     1381 tgtgacgacc acgttggtct ggccggtgac atcggcgccg ttcaatcgcg cccggatggt
     1441 cagaggcgag aacggcagga tcgtacgttc gttgggaagc gcttcgatct cgaggtaaac
     1501 gacgttggag gcgaccgtgt agacgtaggt gtttgtctcg cccggaaaga cgatttgctg
     1561 ggcgtaggtc tggaccacgc gattggtcac caggccgttg gttggccagt cgtcgatcca
     1621 tgtcgagtag acgaacgtgc ccggcgcgac cacgtcgggt ttgaagcggc ccacttcgct
     1681 ttcgaatccg atgccgacgt tgccgcggct cgagaagggc gcgacctggc tgtcgctgtc
     1741 cgtttcggag aaggtgtatt gccctgcctc gatattcgtg accgtgatcg gttcgccgtt
     1801 cgtgtcgatg acgtcgatga tgttggtgac aatgttggtg atatagacga agcgattgag
     1861 ctcggtcgcc cccacggtca ggacgttctt ggccgtgccc ggcgaggtga tggtgccggg
     1921 ctcgccgccc gtgccgacgt cattgcccgc gccgctgttg ccggcggcga agacgaacag
     1981 gagtgcctgc ggaccgggcg tgtagggcag ggcatcgcgc gtggcggcgt cgtagctggc
     2041 ggcggacgag tcgtattcga aacgtcccgg gtaaccccaa ctgttgttcg agatcaggat
     2101 gttggtcagg ctgtgatttg ttccgtaccg cgtgttggcg gcggtctctt gcaggtaggc
     2161 gtccgagatg gtcggtccgc cgtccaggtc gatcggcagc acgaacaggc tggcggcggg
     2221 ggcgatgccg cggaagaccg cgttgctgag cgagccgtac acgttcgtgc cggccgtgac
     2281 gctggtccca agtccgatga tcgtcccggc cacatgggtg ccgtggccat tggtgtcgac
     2341 cagggaggcg gcgtttgtgt cgccgtagat tcggtgctgc ccgcccagca tcaaatcggg
     2401 gtgattggtc ttgacgcccg aatcgttcac attcacgcag atgttcgatc cggtgagggg
     2461 cagggtcaga ttcgtcgatg ccccgccgtc gactgtgttc cagagcacgc ccatgcgcac
     2521 gccggtcagg tcgttcatgg gaagccgcgc gacccacggc tcgaccaact ggacttccgg
     2581 caggcaggcc agcgcggcca cttcgcccgg aggcggctcg accacgaact gcgggccgaa
     2641 tggggatcgc gcctcggcca ggatgttggc gccgatggct tcgagtgcat ggcgcgcgcc
     2701 agccccgctc tcgggcagga gcgtgacgcg caggcgcgcc cggggcggca gcggctgctg
     2761 ctcggcggcg agttcgagga ggctcggatc gatcttgaag taaggctcga agggtatcac
     2821 ggcccgggcc gagccggaga cccggagggc gtgcgccgcc gatgcggacg cgcgcaccag
     2881 ataggcgttg ttcgggatgt acgagacgat gtcggcccct tgatcggcaa gcgcggcgcg
     2941 aaaggcctcg gtgattggcc ccttagcctg gatcacatag ctccccggtt cctcgccggt
     3001 cttgaggtgc ggcggcaagg acaggggaac gtccgaggcg gtgtcgatca gggcgtttcg
     3061 gaggaggatg gcggagtcgc tgtgggcgag ttcgcggatc ggcttggacg tgttgcggac
     3121 gcggcggggg aactgcggat cgaccgtcgg ctctaccgca tcggagtcgg gcgcgatcgc
     3181 ggcggcggat tggtccatgg gcctgaccgc ggcggggagg ggggtgagat tggtgagcaa
     3241 ccggacatct ggcgtcggtt cagcgctttc cgaggctgtc tgagcggttc gggtcaagcg
     3301 gtccgagggt gcgatctttt caagggcagc gcggcgatgg gcgagttgcc agaagccgaa
     3361 tgcgcccagg aggcaggcca gaacgaaaaa tgaccgtgtt cgtgacgtca taaaacggat
     3421 ggaaggtggg atggcccgag gagcgtctcg ctattcgctg aggacctcat tgaagttttc
     3481 gacgacagct cggggggtgt cgagcggccc atcgaggcga acgacggacc gggtgacgca
     3541 ttcgctcttg acctggtagt tggtgcacat gcggttgaag atgccttcgc cgaagtagag
     3601 cgagttgggc gcttccttga gcgtctggcc gaaggcgtag accacgaatc ggggttcatc
     3661 cgccttgagc agcgagagaa tctgttgagg gatgcgctcg agcacttcgt cgcgtggcca
     3721 gaccggcagt ttgcggggga gcatgcctgt gctcccgggg gtggccggtg gactggcgaa
     3781 ggtcagttcc ggcgtggcca ggatgcgtcc gagggaatcg aagtagggcg catggacgcc
     3841 gatcaggagg ttggtttcct gcgcgcgggt gcgattgatg ccatcgacaa tcttctgcag
     3901 ggtgctgagg ttggtagccg agggttcgaa caggtttgat gacacctgat cgaggaagag
     3961 ttcgtcgagg gcgccggtgt cgtcgatggg gaggagcacg ctgactccgc tcaggacggc
     4021 ggcccaggcg gccaggttgt cctgattgac cgagagcagg ccgcgctgcg cgttgtcggt
     4081 gggggcggtg gtgaacaggt cggccagagt ccagtcgttg gttggatggg tgccctggct
     4141 gcccgcccag cggtaccacg aggtctgggt cgtcgtgttg gtgtcggcga tgtcggcctt
     4201 gagatagacc gtctgccacg gggtgccgcg gtggacgcgt ccgatccagc cgacattggg
     4261 gaatttgttg gtgggaaagg cccagtcgtc cgagcgttcg atcagagggt ctttgacccg
     4321 gatatcgcgg gccaggacat cggccgactc gttgggattg ccgccccaag gccggtagcg
     4381 ctggttcaac aaaccgaggt tcgagttggt cagggtgatt tgaggcggga tggcgaaccg
     4441 gacggcgttg gtgcgattgg ggtcgttggg cgggttgaag ggatcgagca gatcgcccag
     4501 ggtgtagtgg accagggggt cattggcctg ccaggtgatt tcctgataga ccttgcgtgt
     4561 gggcgaatag ccggcctgga tggccagttt gcccgcgatc tcctgcgcca aacggtcccg
     4621 ttcccgctgg ctggtgtaag tgaggggtgt cagaccgcag aacagacgca gccggtcgat
     4681 gctccgttcc ttgtcgcgtc cctcggcgga gacggcgctc caactgcgcc actgctgggc
     4741 gctggtggtg atgttgccga gcg
//
LOCUS       DAOQRO010000281         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18838_length_4760_cov_3.602763, whole genome shotgun sequence.
ACCESSION   DAOQRO010000281 DAOQRO010000000
VERSION     DAOQRO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18838_length_4760_cov_3.602763"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKK20_10295"
     CDS             <1..641
                     /locus_tag="PKK20_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNV00317.1"
                     /translation="ISGLASFSNACEPGGSEAFQRHARRMVDAAVLLGVPTLCMQTGM
                     PLPNMGRIEQIRQIVAKVYAPITAYAKEKGIRIAIENWHETCLQGIDTFECLLETIPD
                     DHFGLNYDPSHLVHQECNYLLPVRLFGPRIFHTHAKDTLVDAEARAHVGIYGRGWWRY
                     VIPGAGSIHWGEYINHLRTAGYKGVLSIEHEDSALGREQGFILAARHLAQFC"
     gene            851..1849
                     /locus_tag="PKK20_10300"
     CDS             851..1849
                     /locus_tag="PKK20_10300"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNV00318.1"
                     /translation="MRRLFAFGLAVPESLAILIWIVCCFACNGPCAVRAAESQRTFQN
                     PLLPGNLADPTVIRHGGIFYLYATGEVDGDNGTRAYTSTNLVDWEKGPVVFRPGKPHV
                     WAPDVWRDPASGRFYLYYTVDQTVGVADANNPLGPFTMRRKFFDQAIDAHLFRDEDGR
                     LYLYYAQLPGFHISVQAMSGPSEPSGEPRPLLRPESEWEKRSGQVTEGPWIIKHAGRY
                     YLLYSGSGADTPNYAVGYAIADSPLGPFTRAPHNPIVQRSEGLFGPGHGCAIQDDAGQ
                     WWHLYHQKANDRVEWNRFLCLDRLWFDVEGRLFGRATRGQAQPAPVIHTNGTGPAQ"
     gene            complement(1869..3218)
                     /locus_tag="PKK20_10305"
     CDS             complement(1869..3218)
                     /locus_tag="PKK20_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNV00319.1"
                     /translation="MNDHCGPAAGNRLYLVVICLVAALGGLLFGFDTAVISGTVGFFQ
                     AEFSLSAALKGWVASSALVGCFFGALAAGALSDRFGRKRVMLLSAVFFTVSAAWCGLS
                     GTAVQLVLARLVGGLGIGIASLISPLYIAEVSPPQIRGRLVALQQLAIVLGILGAFSS
                     NSLILHTELSDAAKWRWMLAVGAFPALAFLILIVPVPESPRWLCKQGLEDAARAVLAR
                     VAGSTAAEMELGQIREALALEGGSFSDLFKPGLRRALAVGVTLAVLTQVTGINAIMYY
                     APEIFKQAGAAAAAAYNDTVWIGLTNLVFTLISMAVVDRLGRKPLLLIGASCMGLALA
                     GVGYAFHTGATGHALLLGVLVYVGSFAVSMGVVGWVVISEIYPTRTRGRAMAVATSAV
                     WAACYLVSQTFPILVERIGSAATFWTYGAMCAVAIVFVARFVPETKGRSLEEIERSW"
     gene            complement(3322..>4760)
                     /locus_tag="PKK20_10310"
     CDS             complement(3322..>4760)
                     /locus_tag="PKK20_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNV00320.1"
                     /translation="AVYNDPDDGLDKGGFYGAFIAAPITPALAPFIQARVNDDPRESK
                     RVELFRLPQADNQAAVRFRFAHAGSDSWYWGIDNFGLYSIEQVDPPTITTQPQDQAAF
                     AGDAVSFTAAAGGIGPFTYQWLFNGQPIEGAAAPTLGIERARASDAGTYRVRVTNIGG
                     TTESRAAALVVNPLPDIVAGVWNFGPEGLERASGVGTLEYADGDGTSTLTTFEQGPPP
                     IADTEGTFMRVPAFYDSANGYNLTMPTRPVPTEGYINRYSMAWDILLPWDVNWMPFFN
                     SSPDNRNDADFYVNNAGALGIGALGYSAEGVVQPDTWHRVVFAANLVAGRVTIYIDGS
                     PVHARTGASLANGRFALYSGLDEGPDVRLFNEGDSNGDYTHEVLVSSFAFISRELTPA
                     EVAAMGGPTAAGITLPQPVTPITLGPATLADATITFSWTGGNGPFQVQKKASLSAPSW
                     DNVGDPTTARVATDATTGPAGFYRVIEP"
BASE COUNT          871 a         1442 c         1636 g          811 t
ORIGIN      
        1 agatcagcgg tctggccagt ttcagcaatg cgtgcgagcc ggggggcagc gaggcatttc
       61 agcgccatgc ccgcaggatg gttgacgcgg cggtgttgtt gggtgtgccg acgctttgca
      121 tgcagacggg catgccgctg ccgaacatgg gacgaatcga gcagatcagg cagatcgtcg
      181 cgaaggtgta cgctccgatc acagcctacg cgaaggagaa aggcattcgg atcgccatcg
      241 agaactggca cgagacgtgt ctccagggca ttgacacctt cgagtgtctg ctcgagacca
      301 tcccggacga ccattttggc ctgaactacg atccgtcgca cctggtacat caggaatgca
      361 actacctcct gcccgtgcgt ctgttcgggc cacgcatctt ccacacgcac gcaaaggaca
      421 cacttgtcga cgccgaggcg cgggcacacg tcggcattta cgggcgcggc tggtggcgct
      481 acgtgattcc cggcgcgggc agcatccact ggggcgagta catcaatcac ctgcggacgg
      541 ccggctacaa gggcgtcctg agcatcgagc acgaggacag cgctctgggt cgcgagcagg
      601 gtttcatcct tgccgcgcgg catctggcgc agttctgctg aggcgggtcg ggggcagtgc
      661 gccattgtat gaatcctcca tccgggctac ggctacgcgt cccattgcca ctggcgagac
      721 gacacgacga tcgcgtttca ctgcgacgga ccatcggggc acgggctcta tgagatcacg
      781 ggatgagaac tgcttgggca gacgcgcacc ctatcgacgc gcggcggcct gttgcgctag
      841 agtgcgccgc atgagacgtc tattcgcctt cggtttggcg gtccccgaaa gcctggcgat
      901 cctgatctgg atcgtctgtt gcttcgcgtg caacggccct tgtgcagtcc gcgcggcgga
      961 atcacagcgg acttttcaga atccgctcct gcccggcaac cttgccgatc cgaccgtgat
     1021 tcggcatggc ggcatctttt atttgtacgc gaccggcgag gtcgacggtg acaacggcac
     1081 gcgcgcgtac acctcgacca atcttgtgga ttgggagaag gggccggtcg tctttcgtcc
     1141 gggaaagccg catgtctggg cccccgacgt ctggcgcgac ccggcatcgg gacggttcta
     1201 tctctattac acggtcgacc agacggtggg cgtggccgac gcgaataatc cgctcgggcc
     1261 gtttaccatg cggcgcaagt ttttcgatca ggcgatcgac gcccacctgt tccgcgatga
     1321 agacgggcgc ctctatctct actacgcgca gttgcctgga tttcacattt cggtccaggc
     1381 gatgagcggt ccgtccgagc cttcggggga gcccaggcct ctgctgcgtc cggagtcgga
     1441 gtgggagaag cgcagcgggc aggtgaccga gggcccctgg atcatcaagc acgcggggcg
     1501 gtactacctg ctgtacagcg ggtccggcgc ggacaccccc aactacgccg tcggctacgc
     1561 gattgcggac agccccctgg ggccgttcac ccgggcaccg cacaacccga ttgtccaacg
     1621 ttcggaaggt ctatttggcc ccgggcacgg ctgcgcgatc caggatgacg caggccagtg
     1681 gtggcatctg taccatcaaa aggcgaatga ccgcgtcgag tggaatcgct ttctctgcct
     1741 ggaccgtctc tggttcgatg tcgaggggag gttgttcgga cgcgccacgc gcgggcaggc
     1801 gcagcctgcg ccggtgattc acacgaacgg gaccggccct gcccaataga ttgcgagggg
     1861 aattgcgctc accacgagcg ttcgatttcc tcgaggctgc ggcctttggt ttcgggcaca
     1921 aatcgcgcga cgaagacgat cgcgactgcg cacatagcac cgtaggtcca gaaggtcgcg
     1981 gcgctgccga tacgttcgac caggatgggg aaggtctggg aaacgaggta gcaggcggcc
     2041 cagaccgcgc tggtggccac ggccatggca cggcctcgag tgcgcgtggg gtagatttct
     2101 gaaatgacaa cccagcccac cacgcccatc gagacggcga aactgccgac gtagaccagc
     2161 acgcccaaga gcaacgcgtg tccggtcgcg cctgtgtgga atgcatagcc gacgcccgcc
     2221 aaagccagtc ccatgcagga agccccgatc agcagaagcg gcttccgtcc caaacggtcc
     2281 acgaccgcca tcgagatcaa ggtgaagacc aggttggtca ggccaatcca gaccgtgtcg
     2341 ttgtaggccg cggccgcggc cgcacccgcc tgtttgaaga tctcgggcgc ataatacatg
     2401 atcgcgttga ttcccgtgac ctgggtgagc acggcgagcg tgacgccgac ggccagggcg
     2461 cggcgcaggc ccggtttgaa gagatcggaa aacgaaccgc cttcgagcgc gagcgcttcg
     2521 cgaatctgcc caagttccat ctcggccgcg gtcgatccag ccacacgggc aagcacggcg
     2581 cgcgccgcgt cctcgagccc ctgtttgcac agccagcgcg ggctttccgg cacgggcacg
     2641 atcagaatca ggaacgccag ggcggggaac gcgcccacgg cgagcatcca gcgccatttg
     2701 gcggcgtcgg acagttccgt gtgaaggatc aggctgttgc tggagaacgc gccgaggatg
     2761 ccgagcacga tcgccagttg ctgaagggcg acgagccgac cacgaatctg gggcggagag
     2821 acctcggcga tgtagagagg cgagatcagc gaggcgatgc caatcccgag tcctcccacc
     2881 aaccgcgcca acacaagctg caccgcggtc ccgctcaatc cgcaccacgc ggccgacacg
     2941 gtgaagaaga cggcggacag taacatcacg cgcttgcgtc cgaaacggtc gctgagcgcg
     3001 cccgctgcga gcgcgccgaa gaagcagccg accagcgcgc tgctcgccac ccagcccttg
     3061 agggcagccg agagactgaa ttccgcctga aagaaaccga ccgtccccga gatcaccgcg
     3121 gtgtcgaagc cgaacaagag tccgccgagg gccgcgacga ggcagatgac gacgaggtaa
     3181 agtcggttgc cggcagcggg tccgcaatga tcattcatgg tgcatggaca ggatttggtc
     3241 tgaggcgctt tgcagcccgc atagcagtcc tcttgcgaag tctgagtgcc ctttgcacca
     3301 tcgggatgtg aaatatgcgg gctagggctc gatgacgcgg tagaaccccg ccgggccggt
     3361 tgtcgcgtcg gttgcgacgc gtgccgtggt ggggtcgcct acgttgtccc aggagggagc
     3421 cgacagcgac gccttctttt ggacttggaa cgggccgttg ccgcctgtcc atgagaacgt
     3481 gattgtggcg tcggcgaggg tggcggggcc gagggtgatg ggtgtgacag gctggggcaa
     3541 ggtgatgccg gctgccgtgg gaccccccat ggcggcgact tccgcgggtg tcaattcgcg
     3601 cgagatgaag gcgaagctgc tcaccaggac ttcgtgtgtg taatcgccat tcgagtcacc
     3661 ctcgttgaac agccgaacat ccggaccctc gtcgaggccc gaatagaggg cgaatcgtcc
     3721 gttggccagc gacgcgccag tgcgggcgtg gacgggcgag ccgtcgatgt agatggtgac
     3781 gcggcccgcg acgaggttgg cggcgaacac gacgcgatgc catgtgtccg gctggaccac
     3841 gccctccgcg gagtatccaa gggcgccgat gccgagggcg ccggcattgt tgacgtagaa
     3901 atcggcgtca ttcctgttgt caggagagga attgaagaag ggcatccagt tcacgtccca
     3961 ggggaggagg atgtcccagg ccatggaata gcggttgatg tagccttcgg tcgggacggg
     4021 tcgagtcggc atggtgagat tgtagccgtt ggcgctgtcg tagaaggccg gcacacgcat
     4081 gaacgtcccc tccgtgtcgg cgatgggcgg aggtccttgc tcgaaggtgg tgagagtcga
     4141 ggtgccgtcg ccatccgcat attcgagtgt tccgacgccg ctggcgcgtt cgagtccctc
     4201 ggggccgaag ttccagacac cggcgacgat gtccggcagg ggattgacaa ccagggcggc
     4261 ggcccggctt tcggtggtgc cgccgatgtt ggtgacgcgc acgcggtacg tcccggcatc
     4321 cgacgctcga gcgcgctcga tgcccagggt cggcgccgcg gcaccctcga tcggctggcc
     4381 gttgaacagc cactggtaag tgaaggggcc gatgccgccg gcggctgcgg tgaaggacac
     4441 ggcgtcgccg gcaaaggcgg cctggtcctg gggttgcgtg gtgatcgtgg gcggatcgac
     4501 ctgctcgatg ctgtagaggc cgaagttgtc gatgccccaa taccaactgt ccgagcctgc
     4561 atgggcgaag cggaaccgga cggccgcctg gttgtcggcc tgcgggaggc ggaacagctc
     4621 gacgcgtttg gattcgcgcg ggtcgtcgtt cacgcgggcc tggatgaagg gggcgagggc
     4681 cggcgtaatc ggggcggcga tgaaggcccc atagaagccg cctttgtcga gaccgtcgtc
     4741 ggggtcgttg tacaccgcga
//
LOCUS       DAOQRO010000282         4756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18859_length_4756_cov_4.464369, whole genome shotgun sequence.
ACCESSION   DAOQRO010000282 DAOQRO010000000
VERSION     DAOQRO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18859_length_4756_cov_4.464369"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="PKK20_10315"
     CDS             <1..118
                     /locus_tag="PKK20_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00321.1"
                     /translation="TEKLRAEILVKPKVELVPPGSLPVSEGKARRVIDQRVL"
     gene            complement(262..699)
                     /locus_tag="PKK20_10320"
     CDS             complement(262..699)
                     /locus_tag="PKK20_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV00322.1"
                     /translation="MVKLLIILLIGLVFEAIGVVLLSQGLKQIGPLNRVTAPEVVRIV
                     RRGAANARILLGVFFEALFFASLLVLMSRSDVSFVWPLTSLGFVLTTLAAHLILKEQV
                     SVLRWCGVCLIMLGAGLITWTEHQRSNDAHAFSEGRVPGGPDR"
     gene            complement(656..1564)
                     /gene="hpnK"
                     /locus_tag="PKK20_10325"
     CDS             complement(656..1564)
                     /gene="hpnK"
                     /locus_tag="PKK20_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HNV00323.1"
                     /translation="MSHTTATRRRSEARSIPRRLIVNADDFGRSDAVNQAVIQAHCEG
                     ILTSASLMVNEPACAEAARLARAHPRLGVGLHLTIVRGRAALTPQQAPGLVNPEQAFA
                     HDAIRAGLRYFFKRSLREALRQEIAAQFHRFDETGLVLDHVNGHLNMHLHPTVLRLLC
                     APSGARTIPSIRLTRDLFWTNAHLARGHWVYRLSHAVIFSLLCAWARPRLRRARMRHT
                     RAVFGLLQNGRVDTDYVCRLLRRLPKGDSELYCHPSLTDFRHELDALVSAQAREAVRR
                     CGIQLIRYQDLDHGETADHSSDRPRL"
     gene            complement(1564..2481)
                     /gene="hpnD"
                     /locus_tag="PKK20_10330"
     CDS             complement(1564..2481)
                     /gene="hpnD"
                     /locus_tag="PKK20_10330"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNV00324.1"
                     /translation="MNAPVSQTITRQSASNLALAFILLPPEKRRAMAALYAFCREVDD
                     IADEVSVPIDVRRSRLEQWRSDLHAIYARRQPRLAVMCELAPVVERHRLPRLLFEELL
                     EGVEMDLDVQRYPDYAATELYCYRVACVVGLLSIEIFGYRNPACRDYAIALGKALQLT
                     NILRDVGADADRGRIYLPLDELARCRVTPEEILRHQYSPRFRDLAEAVAARARAHYSQ
                     ARQALPPEDRSAMIAAESMAAVYWSMLEKLERLEFQVLGPTPLRLCRAKKLLLTIRTW
                     LRVKFGSATPNYGVVPTARNSDAPAVECR"
     gene            complement(3121..4560)
                     /locus_tag="PKK20_10335"
     CDS             complement(3121..4560)
                     /locus_tag="PKK20_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV00325.1"
                     /translation="MSLPLPPLLFVDDEKNARIQFPDMVAAMGFDDVSVVTADSAEQA
                     LKLLEQSEYFMVVTDVRLGGMSGYDLLRRARARWPDTPVLVITAYATPRQAVDAIKAG
                     AYDYLAKPYEPEALRQAITRSAEVYRLRKENASLRARSGEVYRLEHIIGESPKMVALR
                     QLIQTVAPTNATVLILGESGTGKELVAGALHSLSHRRERNYIRINCAAIPEALLESEL
                     FGHEKGSFTGALRQKSGRVEEADCGTIFLDEIGDMSRPLQAKLLRFLEDGTFTRVGSN
                     QELRVDVRLIAATNRDIIRAIRENHFREDLFHRLNVVQFMPPPLRERGQDVLLLAEHF
                     LRHFGASMSKSIRGISAPAQQKLLAHHWPGNVRELRNAIERAVILETRDKLQAESLPD
                     SQVENRLRKSGSPPVGEASLDDHLAGYERDLIVNLLEREGFDLNRTAEALRISRTALR
                     TRMQRLDIDADDPTARNAAGSATRKRSVA"
     gene            complement(4557..>4756)
                     /locus_tag="PKK20_10340"
     CDS             complement(4557..>4756)
                     /locus_tag="PKK20_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV00326.1"
                     /translation="IAPENTERIFAPYFTTREKGTGLGLAIVKHNTEMYGGQITVESE
                     LGKGVRFILNFPARTLMKLRK"
BASE COUNT          856 a         1449 c         1635 g          816 t
ORIGIN      
        1 caccgagaag ctgcgcgccg agattctggt caagcccaag gtggaactgg tgccgccggg
       61 cagcctcccc gtgagcgagg gcaaggccag gcgcgttatt gaccagcgcg tgctctgagg
      121 cgggcgcaag accgatgcaa atgtccggga ggcggatcgg cccgcccctg actctctgac
      181 cgaaaggaca gaacgcatcg cattcacgga ctggtgtcgg agggacgtgt cgaccgagtc
      241 ctcggatcga ggcaatcacc gtcaccgatc ggggcctccc ggaactcggc cctccgaaaa
      301 ggcgtgcgca tcgttcgatc gctggtgctc ggtccacgtg atgagcccgg cccccagcat
      361 gatgaggcac acgccgcacc agcgcagaac gctgacctgc tccttcagga tcaggtgagc
      421 agccagcgtg gtcagcacga accccagcga tgtcagcggc cacacgaagc tgacatcgct
      481 gcgggacatg aggacaagca gggaagcgaa gaaaagggct tcgaaaaaca cccccaggag
      541 aatgcgcgca ttcgcggcgc cgcgcctgac gatccggacg acttcaggcg ctgtgacgcg
      601 gttcagcgga ccgatctgtt tgagtccctg gctgaggagc acgacgccga tggcctcaaa
      661 gacgaggccg atcagaagaa tgatcagcag tttcaccatg gtcgaggtcc tggtagcgga
      721 tgagttggat tccacagcgc cgcacggcct cccgggcctg cgcactcact agcgcgtcca
      781 gctcatgcct aaagtcggtc agcgacggat gacagtaaag ctccgaatcg cccttcggaa
      841 gccgccggag cagccggcag acataatcgg tgtccacgcg cccgttctgc agcaggccga
      901 acacggcgcg ggtgtgccgc atgcgggccc ggcgcagacg cgggcgggcc catgcgcaga
      961 ggagagagaa gatgacggcg tggctcaaac ggtagaccca gtgcccgcgc gccagatgcg
     1021 cgttagtcca aaacaaatcc cgcgtcaggc ggatcgaggg aatcgtcctc gccccagacg
     1081 gcgcgcacag cagacgcaga accgtcgggt gcagatgcat gttgagatgc ccgttgacat
     1141 ggtcgaggac aaggccggtt tcgtcgaatc ggtggaattg ggcggcgatt tcctgccgca
     1201 aagcctcgcg gaggctgcgt ttgaagaagt accgcaatcc ggcgcgaatc gcgtcgtgtg
     1261 cgaacgcctg ctccgggttg accaggccag gggcctgctg cggtgtgagc gcggctctgc
     1321 cgcggacaat ggtcaaatgc aggcccaccc ccagacgcgg gtgcgcccgg gcgagccgcg
     1381 cggcctcggc gcatgccggt tcgttgacca tgaggctggc ggaggtcaga atgccctcgc
     1441 agtgcgcctg gatcaccgct tggttgacgg cgtcggaacg cccgaaatca tccgcattca
     1501 cgataaggcg tcgcgggatc gagcgcgcct cggaccgtct ccgggttgcg gtcgtgtgcg
     1561 acatcaccgg cactcgactg ccggcgcgtc agagtttcga gctgtcggaa caacgccgta
     1621 gttgggagtt gcgctgccga acttgacgcg aagccacgtc cggattgtca ggagcagttt
     1681 cttcgcgcgg cagagtcgca gaggcgtggg tccgagcacc tggaattcga gccgttccag
     1741 tttctcgagc atactccaat agacggcggc catcgactcg gcggcgatca tggcagagcg
     1801 gtcctcggga ggcagtgcct ggcgggcctg gctgtaatgg gcgcgggccc gggcggcgac
     1861 ggcctcggcc aggtcgcgga agcgcggaga gtactggtgc ctgagaatct cctcgggcgt
     1921 cacccggcag cgggcaagtt catcgagcgg cagatagatt cgtccacgat cggcatccgc
     1981 gcccacatcg cgcaggatgt ttgtgagctg gagggcctta cccagcgcaa tcgcgtaatc
     2041 gcgacaggca ggattgcgat agccgaaaat ctcgatactg agcaggccga cgacgcatgc
     2101 cacgcggtag cagtagagct cggttgcggc gtagtcggga taacgctgca catcgaggtc
     2161 catctcgacg ccttcgagca actcctcgaa caggaggcgc ggcaggcggt ggcgctcgac
     2221 cacgggcgcg agttcacaca tgacggcaag gcgcggctga cgccgggcgt agatggcatg
     2281 cagatcgctt cgccactgct ccaatcggga tcggcgcacg tcgatcggaa cgctcacttc
     2341 gtccgcaatg tcatccacct cgcggcaaaa ggcataaaga gccgccatgg ctcggcgctt
     2401 ctcgggcggc agcagaatga acgcaagggc caggttcgag gcgctctgcc gcgtgatggt
     2461 ctggctgacg ggtgcgttca tgcctgggtg ggaccgggca atcagccgcc agcgccggct
     2521 tggtcacgag cttttggcat gggcgcggct cacgtgaccg agggcggttg gagcgtggaa
     2581 cggggcggag gcaggccccc ggtttcggcc agagtggggt tgcgcattct gtgctcgcgc
     2641 cgtctccgtt tgcgtctgtg ccggcgatgg tgtctggcgc ggttggagtc ggattctcca
     2701 ctctcctcga tctgggtgcc gcggtggtgc ctcgagtggg agtggggccg ctttcggagg
     2761 aacaaagccc agatcaaaag cacgatcagc agcagaattc ccacgccgag gaccacgagc
     2821 atatcctgcg tcaagcccgg cacgcggctc caggcgggac tgtcactgct gccagtcggg
     2881 ggacctgagt aagtgacttg ggcgagcccg gcatcggaga cgactccgag agttgcgaga
     2941 ccggccagcc agaggacggc ggacccaatc gtggtcaatg gaccccacgg gtgcatcggc
     3001 gcgatttcgg ggcgggcaat cgggccggat cgtcggggcg gtcggatggc ggggcggcgg
     3061 caattcgaca ggctggggtt tggggggcgc gggtggagca ggcaagctgt catggcgcgt
     3121 ttatgcgact gagcgtttcc gagttgcgga tcccgcggcg tttcgggctg tggggtcgtc
     3181 agcgtcgatg tccaaacgct gcatgcgggt gcggagggcg gtgcgactga tgcgcaacgc
     3241 ctcggccgtg cggttcagat cgaagccctc gcgctcgagg agattgacga tgagatcgcg
     3301 ttcatacccg gcgaggtggt cgtccagaga ggcttcgccc acgggtggag agccgctctt
     3361 ccgaagccgg ttctcgacct gagaatccgg caggctctcg gcttgcagtt tgtcgcgagt
     3421 ctcgagaatc accgcgcgct cgatggcgtt gcgcagctcg cggacgttgc ctggccagtg
     3481 atgggccagc agcttctgtt gcgccggcgc gctgatgccg cggatgctct tgctcatgga
     3541 cgccccgaaa tgacggagga aatgctcggc cagcaaaagc acgtcctgac cccgctcgcg
     3601 caggggcggg ggcatgaact gaaccacgtt gagccggtga aagaggtcct cgcggaaatg
     3661 attctcgcgg attgcgcgga tgatgtcgcg gttggtggcc gcaatcagcc gcacatccac
     3721 gcgcagttcc tgattgctcc cgacgcgcgt gaacgtgccg tcctccagaa aacggagcag
     3781 cttcgcctgg agcgggcggc tcatgtcgcc gatctcgtcc aggaagatcg tgccgcagtc
     3841 cgcctcctcg acccgtcccg acttctggcg cagcgcgccg gtgaacgatc ccttctcgtg
     3901 gccgaacaac tcgctttcga gcagggcctc ggggatggcg gcgcagttga tgcggatgta
     3961 gttcctctcg cgccggtggc tgaggctgtg cagcgccccc gccaccaact ccttgccggt
     4021 cccgctctcg ccaagaatca ggaccgtggc gttggtcgga gcaacggtct gaatcaactg
     4081 ccgcaaagcg accattttcg gcgattcgcc gatgatatgt tcgaggcggt agacctcccc
     4141 cgagcgggcg cgcagcgagg cgttctcctt gcggaggcga tagacttcgg cgcttcgggt
     4201 gatggcctgg cgcagggcct cgggttcata cggcttggcg aggtagtcgt acgctccggc
     4261 cttgatcgca tcgaccgcct gtcgaggcgt ggcgtaagcc gtgatgacca ggacgggggt
     4321 atccggccag cgggcgcgcg ctctgcgcag gagatcgtag ccgctcatgc cacccagccg
     4381 gacgtcggtc accaccatga aatactcgct ttgctccagc agcttcaaag cctgctccgc
     4441 cgagtccgcg gtgaccacgc tcacgtcgtc aaaccccatc gccgcaacca tgtccgggaa
     4501 ctggatgcgc gcgttctttt cgtcgtcgac gaaaagcagc ggtggcagcg gcagactcat
     4561 ttgcgcagtt tcatgagcgt tcgcgcaggg aagtttagga tgaagcgcac gccttttcca
     4621 agctcagatt cgactgtgat ctggccgccg tacatctcgg tgttgtgctt cacgatcgcc
     4681 aggccgaggc ccgtcccctt ttcccgtgtg gtgaaatagg gcgcaaagat gcgctcggtg
     4741 ttctcaggag ctatcc
//
LOCUS       DAOQRO010000283         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_18979_length_4738_cov_3.951313, whole genome shotgun sequence.
ACCESSION   DAOQRO010000283 DAOQRO010000000
VERSION     DAOQRO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18979_length_4738_cov_3.951313"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKK20_10345"
     CDS             complement(<1..813)
                     /locus_tag="PKK20_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV00327.1"
                     /translation="MNRRSFLQHSLVLAGGCAAGLRAATAPDGLDRLKPIGQGKGVHP
                     GRVVWVHDPGVTDWKGPGDGHWWEGDRVKQGRVDAMLARAVCELTGEATVSRAWARLF
                     RHFNQSRGKGDVGYRAGERVMIKPNWVGLIYLEGHVNTDTYTFIRRHDYMNTAPQMII
                     ALLDQLVRVGVRPGDITVGDTLACLVHEYYSLLHGAFPEVCYEDCEGKFDRIKSKPSA
                     VPLFWSSRPEGKTGDSVPTSFAEADYLINFACLKAHTAGGVTLCAKNHYGSLV"
     gene            1116..1790
                     /locus_tag="PKK20_10350"
     CDS             1116..1790
                     /locus_tag="PKK20_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV00328.1"
                     /translation="MKVLVIEDEKKIASFVRKGLEAEGFVVEVSHHGDEGYMLGTSRP
                     YDVAVFDIMLPGRDGLSILRNLRDRKIPLPVILLTARGELNERLEGLNLGADDYLTKP
                     FYIEELVARIHAVTRRAAGTPQGILSVGDLSMNLLTREVKRGEAAIELTPREFTLLEH
                     LMRSPGRVLTRAQICERVWEYDFDPGTNLVEVYIQRLRKKVDAEAPVKLIETVRGVGY
                     RLKPRP"
     gene            1787..3301
                     /locus_tag="PKK20_10355"
     CDS             1787..3301
                     /locus_tag="PKK20_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV00329.1"
                     /translation="MKPVSFRFRIALLSALISGLVLGGFGVATWYLLHRQKVEAVDTE
                     IRSLGARHPGWLANRGSFDRLQSSIEFIFGEEHKGRILLLVRDAQGQDLYVSPAWPDG
                     LDPTQIDCSLEDDPAAPNHIATNPPPSPRGPPWRPGGGGGRGGMGRGLGPGGGGTSVL
                     FTKIPRFFTVRAGDSAWRLGILGSNEVRLVVGLNFDQVQAELNRMRNIFAVTLPLALL
                     LVASGGWLVAGRALRPLNTISAMAEGVTAQGLDKRIPASGSDVEITRLVRVLNGMMDR
                     LEASFRQAIRFSADASHELKTPLAVMQGELENALQAAEPGSPVQQVFGNLLEETQRLK
                     RITRGLLLLSQADAGQLQLAIEDVDLSAELEGLIEDARVLAGDSNLAFDVRIRPHVRV
                     QADRSLLQMALLNLLRNAVKYNEPGGALAVALEAGDTQALLTVCNSGPGIQPSDQPRV
                     FERFYRADQTRTGAQVGLGLGLSLAREILHAHRGRLVLVESRPGRTCFEVTLPL"
     gene            3404..3562
                     /locus_tag="PKK20_10360"
     CDS             3404..3562
                     /locus_tag="PKK20_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00330.1"
                     /translation="MLVGTAQLDITPEPGIELAEFAVRLQPATAVLDSLHALGTSFNW
                     QSRLDTTC"
     gene            complement(3626..3898)
                     /locus_tag="PKK20_10365"
     CDS             complement(3626..3898)
                     /locus_tag="PKK20_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00331.1"
                     /translation="MEPMFFLENRSEELAGGPGGQFETASPRLGQIVEEHWRLALVTL
                     FVASICGRQVPDHSMVQPTIAKCGTTPGLATAMNREKTSAFIVRAV"
     gene            3970..>4738
                     /locus_tag="PKK20_10370"
     CDS             3970..>4738
                     /locus_tag="PKK20_10370"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV00332.1"
                     /translation="MEPEVYTLKLAGQAPGESPILFVHGLLHGSWCWPAPWREFFNKR
                     GLTAHCLSLPGRDGAATGRRTLRWTSLDDFADAVVQAAAQFEQCPILVGHSLGAMLIQ
                     MRLHKLNPPAAVLLAPTHPGAFRWATWRRSLRQPGLYFRAHRLGRPRLVIRSLQSCRD
                     WFFSNRTPEEVVENCFARLGEDSYRVCLELLLRPKVVWRALTKTPVLVLGAGQDGCMK
                     RKSVEHVAALYGVQAEFFEDMGHDLMLEPGWERVAVRI"
BASE COUNT          869 a         1525 c         1459 g          885 t
ORIGIN      
        1 caccagcgag ccatagtggt tcttggcgca caacgtcaca ccgccggccg tgtgcgcttt
       61 cagacacgcg aagttaatca ggtagtccgc ctcggcaaac gatgtcggga cgctgtcccc
      121 agtcttgccc tcaggacgac tgctccagaa gagcggcacg gccgatggct tggacttaat
      181 ccggtcgaat ttgccttcgc agtcctcgta gcaaacctct ggaaacgcgc catggagcag
      241 gctgtaatac tcgtgcacca ggcaagccag cgtgtcgccg accgtgatgt cgccggggcg
      301 cacaccgacg cggaccagtt gatccagcag ggcgatgatc atctgcggcg cggtgttcat
      361 gtagtcgtgc cgtcggataa acgtgtaggt gtccgtgttg acatgcccct cgaggtagat
      421 caatcccacc cagttgggct tgatcatcac ccgctcgccg gcgcgatagc ccacgtcgcc
      481 tttgccgcgc gactggttga aatggcgaaa cagccgggcc cacgccctgg acaccgtcgc
      541 ctcaccggtc agttcgcaca ccgcgcgcgc cagcatcgcg tcgacgcgcc cctgtttcac
      601 ccggtcgccc tcccaccaat gcccgtcgcc cgggcctttc cagtccgtga ctccgggatc
      661 atgcacccag accacgcggc ccggatgaac cccttttccc tggccgatcg gcttgagccg
      721 atccagaccg tcgggcgcgg tcgccgcgcg cagcccggcg gcgcagccgc ccgcaaggac
      781 gagcgagtgt tggaggaaag agcggcgatt caacgccggc ctcaagttct cgaggcgacg
      841 agcagcatgt ccagtgggat cggcgcaggg cgttttcatg ccatgccaga ttgtctattg
      901 acgttcgagg actgaagagc caccacggct ttcatcgcag tcatttcacg cccgcgcgtc
      961 cgcaaaatca agggtgaaac atgcggcgag ccctccctca gggtattgac agcacacggc
     1021 ggacgcgcag agtcggccgc ggcggaacga ccgcgaacgg cgcgcggcag acacgcgccc
     1081 ggcacaacat ggtcggccgc ttcggttcaa acgttgtgaa ggtgctggtc atcgaggacg
     1141 agaagaagat cgcctccttt gttcgcaaag gacttgaagc ggaggggttc gtggtcgagg
     1201 tgtcgcatca cggcgacgaa ggctacatgc tgggcacaag ccgcccgtac gatgtggcgg
     1261 tgttcgatat catgctgccc gggcgcgacg gcctgagcat tctacgcaac ctgcgcgacc
     1321 ggaagattcc gctgccggtc atcctgctta cggcccgcgg cgaactcaac gaacggctcg
     1381 agggattgaa tcttggagcg gacgactatc tgacaaagcc gttttacatc gaagagctgg
     1441 tcgcccgcat tcacgccgtc acgcggaggg ccgcgggcac gccccagggc attctcagcg
     1501 tcggggattt gtcgatgaac ctcttgactc gcgaagtgaa acggggtgaa gccgccatcg
     1561 aattgacccc acgcgagttc accctgctcg aacatctgat gcgctcgcca gggcgggtgc
     1621 tgacgcgggc gcaaatctgt gaacgggtgt gggagtacga tttcgatccg gggaccaacc
     1681 tcgtcgaggt ctatatccag cgtttgcgca agaaagtgga cgccgaagcg ccggtcaaac
     1741 tgatcgagac cgtccgcggc gtggggtatc gcctcaaacc ccggccatga agccagtttc
     1801 gttccggttc aggatcgcgc tgctctccgc cctcatttcc gggcttgtcc tgggcgggtt
     1861 cggcgtggcg acctggtatt tgctccaccg ccagaaggtg gaggcggtgg acacggagat
     1921 ccggagtctt ggagcgcgcc atcccggttg gctcgccaac cgcggcagtt tcgaccggct
     1981 ccaaagctcg atcgagttca tctttggcga ggaacataaa ggccgcatcc tcctgctcgt
     2041 ccgggatgcg caaggccaag acctgtacgt ttcacccgcc tggccagatg gtctcgatcc
     2101 gacgcaaatc gattgttccc tcgaagacga tccggcagcg cccaaccaca tcgccacgaa
     2161 tccgcccccg tcgccgcgcg gaccgccgtg gcgcccgggc ggcggcggag ggcgcggcgg
     2221 catgggacgt ggtcttggcc ccggcggtgg tggcacctcc gtcctgttca ccaagattcc
     2281 ccgtttcttc acagtccggg ccggcgattc ggcgtggcgg ctgggcattc tgggcagcaa
     2341 cgaagtgcgc ctggtcgtcg ggctcaactt cgaccaggtc caggccgagt tgaaccggat
     2401 gcggaatatc ttcgccgtga ccttgccgct tgctctcctg ctggtggcca gcggcggctg
     2461 gctggtggcg gggcgcgccc tgcgtccttt gaacaccatc agcgctatgg ctgaaggggt
     2521 gacagcgcag ggactggaca agcgcattcc cgcgtcgggc agcgacgtcg aaatcacgcg
     2581 tctggtccgt gtgctcaacg ggatgatgga ccggctggaa gcgagcttcc gccaggcgat
     2641 ccgtttcagc gccgacgcct cgcacgaact caagacgccg ttggcggtga tgcagggcga
     2701 actcgagaac gcgcttcagg cggccgagcc gggctccccc gtccaacagg tctttggcaa
     2761 cctgctcgag gaaactcaac ggctgaagag aatcacccgc ggcctgctgc tgctctccca
     2821 ggccgatgcc ggccaactcc agctcgcgat cgaggatgtg gacttgagcg cagaactcga
     2881 aggactgatc gaggacgctc gcgtccttgc cggcgactcg aacctcgcgt ttgacgttcg
     2941 catcaggccg catgtgcgag ttcaggccga tcgcagcctt ctgcagatgg ctctgctcaa
     3001 cctgctccgc aacgccgtca aatacaacga accgggcggg gcgctcgctg tcgcactcga
     3061 agccggagac acccaagccc tcctcacggt ctgcaacagc ggaccgggca tacagccgtc
     3121 cgatcaaccc cgtgtcttcg agcgattcta tcgcgccgat cagacgcgca ccggagctca
     3181 ggtcggcctg ggattgggtt tgagcctcgc gcgcgagatt ctgcacgccc accgcgggcg
     3241 cctggttctt gtcgagagcc gccccggcag gacctgtttc gaggtgacac tccccctcta
     3301 gccttcatat ttcgcacccc tctggtgcac atggcactca gactccgcta caggactgct
     3361 gtgcgggctt gagaagcaaa agccgtccgg atgcaactct gaggtgcttg tcggcacagc
     3421 ccaactcgat atcacacccg agcccggcat cgaactggct gaattcgccg tgcgcctgca
     3481 gcctgcaacc gccgtgctcg attccctcca cgctttgggg acaagtttca actggcaatc
     3541 gagacttgac accacatgct gaagagcgga ctgcatcgcg cagaacggcc cgcatctccc
     3601 ttgaactgga ggctctcgcc ttgggtcaga ccgccctcac aatgaacgcc gaggtctttt
     3661 cgcggttcat cgctgtggcc agtcccggcg ttgtgccgca cttcgcgatc gtcggctgca
     3721 ccatggaatg atcgggcacc tgccgaccac aaatgcttgc gacgaagaga gttacaaggg
     3781 cgagtcgcca atgctcttcc acaatctgcc ccaatctcgg cgaggcggtc tcgaactgac
     3841 cgcccggacc gcccgcaagc tcctcagatc gattttctaa aaaaaacatc ggttccactc
     3901 gtaacatttc ttgcagaatt ccctataata ttacgtagag atgcatagtg ggtgtttact
     3961 agattcgaga tggaacctga agtctatact ttgaagttgg ccggtcaggc gcccggcgag
     4021 agccccattc tcttcgtgca cggcctattg cacggttcat ggtgctggcc cgccccctgg
     4081 cgcgagtttt tcaacaaacg gggtctgact gcccactgtc tgagcttgcc cggacgcgat
     4141 ggagcagcca ccggaaggag gacgctgcga tggacctcgc tggacgactt tgcggacgcc
     4201 gtggtgcagg ccgcggcgca gttcgaacaa tgtcccatcc tcgtgggcca ttcgctgggc
     4261 gccatgttga tccagatgcg tttacacaag ctgaatcccc cggcggcagt tctgcttgcc
     4321 ccgacgcatc ctggcgcctt caggtgggcg acctggcgcc gatctctgcg ccagccgggc
     4381 ctgtactttc gggcgcaccg cctcggaaga ccccgccttg tcatcagaag cctgcaaagt
     4441 tgccgcgact ggttcttctc caacagaacc cccgaagagg tggtcgagaa ctgctttgcc
     4501 cggctcggag aggattccta ccgcgtctgc ctcgagttgc tgttgcggcc aaaggtggtt
     4561 tggcgggccc tcaccaaaac gcccgtgctc gtgctgggcg ctggacagga cgggtgtatg
     4621 aagcgcaaat cggtcgagca cgtggcggcc ctctacggtg tgcaggcgga gttcttcgag
     4681 gacatgggac acgacctcat gctcgaaccg ggatgggaac gggtggcggt tcgcatcc
//
LOCUS       DAOQRO010000284         4640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_19577_length_4640_cov_3.919956, whole genome shotgun sequence.
ACCESSION   DAOQRO010000284 DAOQRO010000000
VERSION     DAOQRO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19577_length_4640_cov_3.919956"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1726
                     /locus_tag="PKK20_10375"
     CDS             146..1726
                     /locus_tag="PKK20_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00333.1"
                     /translation="MKGTLLTALLLSAFLAHSTATTASSPVALDLWSLAREQSDAHRF
                     STLFTAQDVRGRLANESGLAAAIEWCKRTAVTQVYIECFRDGYQADRTTLSNAMARFR
                     AEGFGVSGCVTPTHVGKRSTGWNVISCYTDPATQERVKAIFAFAASLSDEIMIDDFWF
                     TDCTCAACDAARQARTVTLGARTYPVNGDAWEDYRCELMVRLSRDCALRVAKEVNPKA
                     CLIIKYPQWYDRFHERGYEVLRQTHDFDRTWVGTETRDYRDPRWGGTVQYEAYFIMRW
                     LGNLSGAKCGGGWFDPYGTTERTYIEQARQTVLGGARESLLFCYGSLQGETGPRNVEA
                     LRAEIPDLFAVAREVGRRPVVGVAAYKPANSHPENEARVFDFVGMLGVPLVPCHEFPA
                     QAAAAFFSVHALKDPGFATKLEGFIRAGKPVLVTDGLAARLTNQVDLTAQNVQVLPVH
                     GDPKSLLALPQETLDRLRQPLLRPFETGFRAPNQVGLYLFKDGSWVIENFNDQPAQVE
                     LNGRKLVVEPRGWQKMWK"
     gene            2071..3282
                     /locus_tag="PKK20_10380"
     CDS             2071..3282
                     /locus_tag="PKK20_10380"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNV00334.1"
                     /translation="MRLRQCHALLGLVIAGLAGLWTALAAETDKTADLPDRRISAALQ
                     YPGITLGPDDTLNVDFLIKNRGRSDETVLVEIIEKPEDWTAEIKRYGTVVSGIFVASR
                     EEQTLNLTGAPANKETKKLPPGTYRFAVKAQSADGELTQGSAIVVTVVSAEKASERVS
                     IETSYPTLRGPSGDKFQFSLDVRNDTGQDAVFNFRATTPPGWEASFKPAYESKQISSL
                     QIAANASKTVEMEVTPPYNAQAGEYEFKVEAQSPKARAETDVRVVLTGTYGLKVGTSS
                     GLLSLVTERGRKSTVTLLVQNSGSAVQHKVRFEAFKPENWKVEFTPQTIEELEPGAIK
                     QTEISITPAEEALIGDYSVSLQTEGEKANQETELRITIRAASAWGWIGVGIIALVILG
                     LAAAFRILGRR"
     gene            3327..4067
                     /locus_tag="PKK20_10385"
     CDS             3327..4067
                     /locus_tag="PKK20_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV00335.1"
                     /translation="MAIIQTDQLTRRYGRQTAVDGLSIEIGERDIFGFLGPNGAGKTT
                     TILMLLGLTEPTSGRARVLGFDPARDPLQIKQQVGYLPENVGFYDDLTGCENLRYMGR
                     LNRLPEAQAPALIEEALSTVGLEGEGDKLVGAYSRGMRQRLGIAELLIKNPKLLILDE
                     PTLGLDPDGTNRMLELIVSLRRERGMTVMLSSHQLQQVQRICTRVGIMIKGKLVALGS
                     LDELSQGSPGDKARPNLEEIYMRYFKEA"
     gene            4071..>4640
                     /locus_tag="PKK20_10390"
     CDS             4071..>4640
                     /locus_tag="PKK20_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV00336.1"
                     /translation="MWTICRKELADHFGSIRFLILFCLILMVALVSTYMAATHVREAL
                     DGVSKPSQVFLLLFTTPGKFFSLVQFIAFFGPLLGIIMGFDAINRERQYRTLGKLVSQ
                     PIYRDAIVNGKFLAGVLTISIALLSMMLLLTGMGLMTVGVIPGIEEIGRLAVYLLVSI
                     AYVSFWLGLAILSSVLFRSLATSALAAVAL"
BASE COUNT          887 a         1495 c         1413 g          845 t
ORIGIN      
        1 gctcctgaga ttccccgccc tgacgcgtgc gcaggcgtac gaatgcctag ccttccatga
       61 ggatcaccgc gcggagattg acgcgcttgt cgccgagcag atggttgatt gaaaccacgt
      121 gtgcgagtgt gctatactcg gcgctgtgaa agggaccctt cttactgctc tgctcctcag
      181 tgccttcctg gcccactcca cagccaccac cgccagttcg ccggtcgccc tcgatctctg
      241 gagcctcgcc cgtgagcaat ccgacgccca ccggttctcg acgctgttta cggcgcagga
      301 cgtccgcggc cggttggcaa acgagagcgg cctggccgcc gcgatcgagt ggtgcaagcg
      361 cactgccgtc acgcaggtct atatcgagtg cttccgggat ggctaccagg ccgaccgcac
      421 cacgttgtcc aatgccatgg cacggtttcg cgccgagggc tttggtgtgt ccgggtgcgt
      481 gacgcccacg catgtcggca agcgctccac cgggtggaac gtcatctcct gctacaccga
      541 tcccgccacc caggagcggg tcaaggccat cttcgcgttc gccgccagct tgtcggacga
      601 gatcatgatc gacgacttct ggttcaccga ttgcacctgc gccgcctgcg acgccgcgcg
      661 acaggcccgc acggtcacgc tcggcgcgcg cacctatccc gtaaatgggg atgcgtggga
      721 ggactatcgt tgcgagctga tggtccgact ctcccgtgat tgcgcgctcc gtgtggccaa
      781 ggaggtcaat ccaaaggcct gcctgatcat caagtatccg caatggtatg accgcttcca
      841 cgaacggggg tacgaggtcc ttcgccagac gcacgatttc gatcgcacgt gggtcggcac
      901 cgagacgcgg gactatcggg acccgcgctg gggcggaacc gtccagtacg aggcgtattt
      961 catcatgcgt tggctgggaa atctgagcgg ggcgaagtgc ggcggtggct ggttcgatcc
     1021 ttacggcacg accgaacgca cgtacatcga gcaggcacga cagacggtcc ttggcggagc
     1081 ccgcgaatcg ctcctgttct gctacggcag cctccagggc gagaccggac cgcggaatgt
     1141 cgaagccctg cgcgccgaaa tcccggacct cttcgcggtc gcgcgggaag tcgggcggcg
     1201 tccggtggtg ggagtggccg cgtataaacc ggccaacagc caccccgaga acgaagcgcg
     1261 ggtgttcgat ttcgtgggga tgctgggggt gccgctggtc ccgtgccacg agtttcccgc
     1321 gcaggccgcc gcagcctttt tctccgttca tgcgctgaag gaccctggct ttgccacgaa
     1381 gctcgaggga ttcatccgtg ccggcaagcc ggtgctggtc acggacggct tggccgcgcg
     1441 gctcacgaac caagtcgacc taacggcgca gaacgttcaa gtgctccccg tccatggtga
     1501 ccccaaatcg cttctcgctt tgccccaaga aacgttggac cgtctccgcc aacccctgct
     1561 gcggccgttc gagaccggct tccgcgcgcc aaaccaggtc ggtctttatc tcttcaagga
     1621 cggcagttgg gtgatcgaaa acttcaacga tcagccggcg caggtcgaac tgaacgggcg
     1681 gaagttggtg gtggaaccgc ggggatggca gaagatgtgg aagtgacaga ggcgaggccg
     1741 cgaagtccgg cacgtctctg gcaactgttc gaatcttcaa tcgcgaatga acgccaatgg
     1801 gcacgaatag cccgacaaga gtccagagcg ttctcctgtg gcgccagcca cattcgcatg
     1861 cattcgcgcg gtgaatcgtc atcaacgtga aacgcccaac gttcaactcg caaccatcaa
     1921 tttgagaaag ccaactcgac ttgacaaggc cgagtcgctt gtacaaataa aacgccgatt
     1981 cgagttgcgg ccgcgcgcgg gatcgcacgc tgggtctgac gcctgccccg cccgggctgc
     2041 cgataggatt tgaagttgag gaacggactt atgcgattgc gacaatgcca cgctttgctc
     2101 ggcctggtca tcgccggcct cgccggcctg tggaccgcgc tggcggccga gacagacaag
     2161 accgcggatc tcccggatcg gcgcatttcc gccgcgctgc agtaccccgg catcacgctt
     2221 ggccccgacg acactctgaa cgtcgacttt ctgatcaaga accgcggacg cagcgatgag
     2281 accgttttgg tcgagatcat cgagaaaccg gaagactgga ccgccgaaat caaacgctac
     2341 ggaaccgttg tgagcgggat tttcgttgcc tcgagagaag agcagacgtt gaacctcacg
     2401 ggcgcgcccg cgaacaagga aaccaagaag cttccccccg gcacatatcg tttcgccgtg
     2461 aaggcccagt cggccgacgg cgagctcact caagggtccg cgattgtcgt gacggtcgtc
     2521 tccgcggaga aggcgtccga gcgcgtcagc atcgagacgt cctatccaac tctgcggggg
     2581 ccctcgggcg acaagttcca gttctctttg gacgtgcgga acgacacagg ccaggacgcc
     2641 gtgttcaact ttcgcgcgac gacgccgccg ggctgggagg cgtcgttcaa gcccgcgtac
     2701 gagtccaagc agatcagttc gctccagatc gccgccaacg cgagcaagac ggtcgagatg
     2761 gaggtgaccc cgccctacaa cgcccaggcc ggcgaatacg aattcaaggt cgaggcgcaa
     2821 tcgcccaaag ccagggccga gacggatgtg agggtggttc tcacaggcac ttatggtcta
     2881 aaggtgggca cttcgagcgg cctgctgtcg ctggtcaccg agcgcgggcg caagagcact
     2941 gtcacgttgc tggtgcagaa ctcaggctca gccgtacagc acaaggtgcg attcgaggca
     3001 ttcaagcccg aaaactggaa ggtcgagttc acgccccaga cgatcgagga attggaaccg
     3061 ggcgccatca agcagacgga gatcagcatc acgcccgccg aagaggcgct catcggcgat
     3121 tattcagtca gcctccagac cgaaggcgaa aaggccaacc aggagaccga actgcgcatc
     3181 accatccgcg ccgcatcggc ctggggttgg atcggcgtgg gaatcatcgc gctcgtcatc
     3241 ctcgggctcg ccgccgcctt ccgcatcctc ggacgccgtt gatcgcatcc cgtcatgacc
     3301 cggaccgcat cgggtgccgc gatgctatgg ccatcatcca aaccgaccaa ctgacccgcc
     3361 gctacggacg ccaaacggcg gtggacggac tgtcgatcga gatcggcgag agggacatct
     3421 tcggcttcct cggacccaat ggcgcgggca agaccaccac catcctcatg ctgctcggtc
     3481 tcaccgagcc cacctccgga cgcgcccgag tcctcggatt cgacccggcg cgcgacccgt
     3541 tgcagatcaa gcagcaggtt ggttacctgc ccgagaacgt cggtttttac gacgatctca
     3601 caggctgtga gaacctgcgc tacatgggcc ggctcaaccg gttgcccgaa gcccaggccc
     3661 ccgcgctcat cgaggaagcg ttgtccaccg tcggactcga gggcgaaggg gacaagctgg
     3721 tgggcgctta ctcgcgcggc atgaggcagc gattggggat tgcggagttg ctgatcaaga
     3781 atccgaagct gttgattctg gacgagccga cactcggcct cgatcccgac ggcacgaaca
     3841 ggatgctcga actcatcgtc tccctgcgcc gcgagcgcgg gatgacggtc atgctctcgt
     3901 cccaccaact ccagcaggtg cagcgcatct gcacgcgcgt gggcatcatg atcaagggca
     3961 aactggtcgc gctgggcagc cttgatgaac tgtcccaagg cagtcccggg gacaaggcca
     4021 ggccgaatct cgaggagatt tacatgcgat acttcaagga ggcctgagct atgtggacca
     4081 tttgtcgcaa ggaactggct gatcacttcg ggtcgatccg attcctgatt ctcttctgcc
     4141 tcatactgat ggttgcgctg gtgtccacct acatggccgc cacacacgtc cgcgaagccc
     4201 tcgacggcgt gtccaagcca tcccaggtgt tcctcctcct gttcaccacg cccggcaagt
     4261 tcttctcact cgtccagttc atcgcattct tcggaccgct cctcgggatc atcatgggat
     4321 tcgacgccat caatcgggag cgacaatacc ggaccctggg caagctggtc tcccagccca
     4381 tctaccgcga cgccatcgtc aacggcaagt tcctcgccgg cgtcctgacc atcagcatcg
     4441 ccctgctcag catgatgctc ctgttgaccg gcatgggcct catgaccgtc ggcgtcatcc
     4501 cgggtatcga ggaaatcgga cgtctggccg tctatctcct ggtcagcatc gcctacgtgt
     4561 cgttttggct cggattggcc atcctctcct cggtcctgtt caggtcgctg gcgacctccg
     4621 cgctcgcggc cgtggcgctt
//
LOCUS       DAOQRO010000285         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_19935_length_4587_cov_5.498235, whole genome shotgun sequence.
ACCESSION   DAOQRO010000285 DAOQRO010000000
VERSION     DAOQRO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19935_length_4587_cov_5.498235"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..2837)
                     /locus_tag="PKK20_10395"
     CDS             complement(60..2837)
                     /locus_tag="PKK20_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNV00337.1"
                     /translation="MKAAAAADKPVPRLAFEPRVATRLEFGGFVGERIEAMVDHWLLR
                     APGANPGMLEMFRLRDREPAPQIVPWAGEFVGKYLISAIQTLPLSARADLRALASDVV
                     AQLIATQADDGYLGPFRKPERLRGHWDLWSHYHALLALLMWHEATGDPAALASARRAA
                     DLVCATYLDTGHRLLDAGSDEMNLAIVHGLGWLHRVTGEDRYRRMMAEIEKDWERAGD
                     YLRTGLDGLEFWQSPRPRWESLHDLQGLVELYRITADARYREAFEHHWRSIARWDARN
                     TGGFSSGEQATGNPYAPTPIETCCTIAWMALSIDMLALTGDPRVADALELATLNAWAG
                     AQHPSGRWCTYSTPMDGAREASAHAIVFQSRAGTPELNCCSVNGPRGWGMLAEWAVMT
                     STEGVVLNWLGPLVATVALPDQSRLTLRVTGDYPWQLETDMAIEPEHEQPFTLRIRVP
                     AWSTAFSATCNNELLPPPAPGAYLAVNRVWRKGDRVSLKLDPHLRAVAGAREARGCVS
                     LYRGPILLAWDQRLHDFDENNLPPIDLSRLSQARVTAADNAGTPAWRRQLDPRLVLDL
                     PTANGPTLHLCDFASAGATGTRYRTWLRAESPLPPPVVTRTPRDGARIAGGKVAFRWT
                     RTARLDDSVIAQTLRISLTEDFRTPTLELPGLREAQTLVDIDARFEPDRWYFWDIVTA
                     NDAGQTRSPGPPARFRIDPSLPPAQETLDPPHATGPRGEWVSAALRGNPTPQVGQWQP
                     STGWRSAPGPDGANDGAVALDGNSERLVYTLGGWPENDYSLSIRFKIRSLPSGRIGQI
                     FSAWCAPMDDPLRLVIDDGRLFARIEAGSLTSTQGVPIEPGRWHHATVVKAEDRLTLF
                     LDGKEVTSTPASALISTRATDCALGGNPHYTGNEFLAIDLADFAFFPRALGPDEVGAL
                     GRAAASQSESR"
     gene            3621..4328
                     /gene="rsmI"
                     /locus_tag="PKK20_10400"
     CDS             3621..4328
                     /gene="rsmI"
                     /locus_tag="PKK20_10400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNV00338.1"
                     /translation="MESTALPELRPGTLYLVATPIGNLEDITCRALRVLRECDVVVAE
                     DTRHSGQLLHHFEIRRPLLSCFHFNEARRSEQVLERLREGGKVALITDAGSPGISDPG
                     ERVVRAVLDAGFRVEPVPGPCALVAALTASGLPTDEFHFVGFLPHKSGQRRRELDRLS
                     GVPGTLVLYESPYRVARLLGELAEVFPARQVVLARELTKKFEEFLRGTPVELIERAKA
                     RALRGEFVVLIAPGQNP"
BASE COUNT          819 a         1355 c         1567 g          846 t
ORIGIN      
        1 tgaaggaatc tgtgaaaacg tgaagccgta ttgcagacct ccgtgtcagc gcaggcccgc
       61 tatcgactct ccgattggga cgccgccgct ctcccgaggg ctccgacttc atccggtccc
      121 agagcgcgcg ggaagaaggc gaagtcggcc aggtcaatcg ccaggaactc gttgccagtg
      181 taatgggggt tgccgcccaa ggcgcagtcg gtggctcgtg tcgagatcaa cgccgacgcc
      241 ggggtcgagg tcacttcctt gccgtcgagg aagagggtca atcggtcttc ggccttgacg
      301 acggtggcgt ggtgccagcg accgggctcg atcggcactc cttgggtcga agtcaggctt
      361 cctgcttcga tccgcgcaaa taaccgccca tcgtcgatga ccaggcgcag aggatcatcc
      421 atgggcgcgc accatgcgct gaagatctgg ccgattcggc cggacggcag gctccggatc
      481 ttgaatcgga tggacagcga atagtcgttt tcaggccagc cgcccagagt gtagacgagg
      541 cgttcgctgt tgccgtcgag ggcgacagcg ccatcgttgg cgccgtcggg tccgggcgcg
      601 gatctccagc cggtcgatgg ttgccattgt ccaacttgcg gcgtcgggtt gccgcggagg
      661 gcggcgctca cccattcgcc ccgaggccct gtcgcatgcg gcggatcgag tgtttcctgt
      721 gccggcggca gactcgggtc gattcggaat cgcgcgggtg ggccggggct gcgcgtttgg
      781 cctgcgtcgt tcgccgtgac gatatcccag aaataccaac gatccggttc gaaacgggcg
      841 tcgatgtcga ccagggtctg ggcctcgcga aggcctggca gttcaagcgt cggcgttctg
      901 aagtcttcgg tgaggctgat tcgcagtgtt tgtgcaataa cagaatcatc gagacgggct
      961 gtgcgtgtcc aacggaacgc gaccttgcca ccggcaatgc gcgcgccatc gcggggcgtg
     1021 cgtgtgacca cgggcggagg caatggcgat tcggcgcgga gccaagtgcg gtagcgcgtg
     1081 ccagttgccc ctgcgctggc gaaatcgcag aggtgcagcg tgggaccatt ggcggtgggt
     1141 aggtccagga ccagacgcgg atcaagctgt cttcgccagg caggtgtgcc ggcattgtcg
     1201 gccgctgtaa cccgtgcttg gctcaggcgt gacaggtcga tcggcggcag attgttctca
     1261 tcgaaatcat gcagtcgctg gtcccaggcg agaaggatgg ggccgcggta gagcgagacg
     1321 catcctcgag cctcgcgggc ccccgcgacg gcgcgcaggt gcggatcgag tttcagcgac
     1381 acccgatcgc ctttgcgcca aacgcggttg acggccaggt aggcgcccgg cgcgggcggg
     1441 ggcaggagtt cgttgttgca cgtcgccgag aaggccgttg accacgctgg aacgcggatg
     1501 cggagggtga atggctgttc gtgctcgggc tcgatggcca tatccgtctc gagttgccac
     1561 gggtagtccc cggtgacgcg gagagtcaga cgcgattgat cggggagggc caccgtggcg
     1621 acgagcgggc cgagccaatt caggacgacg ccctctgttg atgtcatcac ggcccattcg
     1681 gcgagcattc cccagccgcg gggtccgttg accgagcaac aattcaattc gggtgtgccg
     1741 gcgcgggatt ggaagacgat tgcgtgggcg gacgcttcac gggcgccgtc catgggcgtg
     1801 ctgtatgtgc accagcgtcc gctggggtgt tgagcgcctg cccaggcgtt gagcgtggcg
     1861 agttcgagcg catcggccac gcgcgggtcc cctgtaagcg cgagcatatc gattgagagc
     1921 gccatccagg caatggtgca acaggtctcg ataggggtcg gagcgtaggg gttgccggtg
     1981 gcctgttcgc cggacgagaa accgcccgtg ttgcgggcgt cccaacgcgc gatgctgcgc
     2041 caatgatgct caaaggcctc ccggtaacgg gcatcggccg tgatacggta aagttcgacc
     2101 agtccctgga gatcgtgcag gctttcccag cgcggacgcg gcgattgcca gaactcgagt
     2161 ccgtcgagcc ctgtgcgcag gtagtcgccg gcgcgttccc aatccttctc tatctcggcc
     2221 atcatgcggc ggtagcggtc ttcgcccgtg acgcgatgca gccagccgag gccgtggact
     2281 atggccagat tcatctcgtc ggagcctgca tcgaggagcc ggtgtccggt gtcgagatat
     2341 gttgcgcaga caagatcggc tgcgcgacgc gcggaggcga gcgcggcggg atcgcccgtg
     2401 gcctcatgcc acatgagcag cgctagcaag gcgtggtaat ggctccagag atcccagtgc
     2461 ccgcgcagac gttcgggctt gcggaacggt ccgagatagc cgtcgtcggc ctgggtagcg
     2521 atcagttgcg cgacgacgtc cgacgcgagg gcgcgcagat ctgctcgagc gctcaacggg
     2581 agcgtttgga tggctgagat caggtatttg ccgacgaatt cgcccgccca aggcacgatt
     2641 tgcggcgcgg gctcccgatc gcgcagccgg aacatctcga gcatgccggg gttcgccccg
     2701 ggggcacgaa gcagccagtg atcaaccatc gcctcgatcc gctcgccgac gaaccctccg
     2761 aattccagtc gtgtggcgac ccgaggctcg aaggccaggc gcggcacagg cttgtccgcc
     2821 gccgctgcag ctttcacatg cattgaagcc gcaggcagga ggagagccgc ggcgcaacag
     2881 gcaaaagctg acgacgcaag gtccatgatg cgatggattt gttttcggga cgccaacccc
     2941 agtcccggca cagcccggtc gtggcactga acggctttgg cgatctccca ctcggatggc
     3001 aatgagaagc aacagatcat aggctgtatc ggctcggcgg ttcccgtaac agaatgagac
     3061 catccagtgc gctcaatccc ctgcgctggc ggcgaatccg gcattcgacc agccgggcgt
     3121 atccgaaacg gagacgcgcg gcggtggttg aggcgactgg ttcggcgatc aatctcagca
     3181 tgggatcacg gatcgcggcg cgattcaact gcagagacac caaggcatgc agagaacacg
     3241 cggttgggaa tcgccgctct tttcgtgctc ctgtgccctc aaaactgccg agctgacgag
     3301 gaatctcgga gggccgagtt cctcgaggtc ctgtttggtg acggtgattg tctcgagcca
     3361 gatgccaccc gcacggtctt ggtcacctca aactcgactc aagcgatttt gagtaggcgt
     3421 ttgattggct tgagtaggcc ccacgcaatc ggtcaaaggc cgttagcttt cattcagccc
     3481 caccacgaga cacgcgaaga gagcgcgttt tccgcctcaa ccgggccgca agcgcgccat
     3541 ggttccttgc agtccaccat tgaccgcttt gcgcggggaa tatgctcccc aaggcgtctt
     3601 ttccgtgaat gaatgggcgc atggaatcga ctgccttgcc tgaactgcgt cccggcacgc
     3661 tctatctggt cgcaacgccc atcggcaatc tcgaagacat cacgtgtcgc gccttgcggg
     3721 tgctgcgcga gtgcgacgtc gtggtggcgg aggacacgcg acattcggga caactgctgc
     3781 atcacttcga gattcgcaga cccctgctga gctgtttcca tttcaacgag gcgcgacgca
     3841 gcgaacaggt cctagagcgt ctgcgtgagg gcgggaaggt cgcgctgatc accgacgcgg
     3901 ggagccctgg catcagcgat ccgggcgagc gggtggtgcg ggctgtgctc gatgcgggtt
     3961 ttcgtgtcga gcctgtgccc ggcccgtgtg cccttgtggc ggccctgacg gccagcgggc
     4021 tgccgacgga cgagttccac tttgtcggct ttctgccgca caagtccggg cagcggcgaa
     4081 gggaactcga tcgtctgagc ggcgtgccgg gcacgctggt gctctacgaa tcgccctatc
     4141 gtgttgcgcg gctgctgggc gagttggccg aggtcttccc cgcgcgccag gtcgttttgg
     4201 cgcgggaact gaccaagaag ttcgaggaat tcctccgcgg tacgccggtt gagttgatcg
     4261 agcgagccaa ggcgcgcgcg ttgcgtggtg agttcgtggt gttgatcgcg ccggggcaga
     4321 atccttgacc ggaaaggctt ccaaaccgca tattgcgcag cgcggcaaag ccgcaaccaa
     4381 attgggccac aactgacggc ctttgcggga tgaaacttct ttcaagaaga aaagaagttt
     4441 acggagagtg gtacaccccg ttggtgcaca ggacggtcag attgcgcaag atgtcttgta
     4501 tgcgggctag agaagcaaaa ccgtgccagg ttcgagcgat agagaactgc accacactgt
     4561 tcccatgaga gcccattctc agggccg
//
LOCUS       DAOQRO010000286         4544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_20200_length_4544_cov_4.088216, whole genome shotgun sequence.
ACCESSION   DAOQRO010000286 DAOQRO010000000
VERSION     DAOQRO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20200_length_4544_cov_4.088216"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3396)
                     /locus_tag="PKK20_10405"
     CDS             complement(<1..3396)
                     /locus_tag="PKK20_10405"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00339.1"
                     /translation="MQHCLALLSACLAVELTLWSIRADVVMNEIHHSPDVKTEPVEFI
                     ELYNSGTTDVDLSGWAFTAGIDFVIPNGTTLPAGGYLVVAQNPSALKSKFGVDALGPW
                     KGRLAGEGEKVELRDAARQIVDSVDYRLGFPWPTVGDAPGYSMELIHPEIDNDLGGHW
                     RASFVGGGQQQSTLLFDAGAEWRVFKGTQPPPANWRDLAFNDSTWIGGPAPVGYDPSV
                     RMGTPLDDMRSGYLDFFIRRTFRVVDAASVTSLSLEALYDDGFKLWINGNNLLNLAMP
                     NGEVPYNQAANPARENNAYDRHEIALPPGLLREGDNVIAVQVANNNLGYSSDAFFDCR
                     LRATVGPTGIGPTPGRLNATFTARIPPAIRQVEHQPRQPGSGRPVRISAKVTSASGLA
                     HVTLEYQAIAPGNYIELSDESYASTWTAVSMNDAGLEGDAQAGDDIYTATLPASLQQH
                     RHLVRYRFKAADLAGVSVSAPYPDDPQPNFAYFCYDGVPAWTGAVCPGAAGALGRTFI
                     VGEEEMNRLPVYHLIAKRKAVEDCTWYDRSHGDEYFWQGTLVYDGRVYDHIHFRPRGG
                     VWRYAMGKNMWKFNFNRGHDFEPRDNWGRKLKTAWTKLNLGANIQQGDYMHRGEQGLF
                     ESVGFRLFQLVGLPSMHTMFVQFRIIDAPQETVPNDQYSGDFWGLYLAIEQPNGRFLD
                     EHGLADGNFYKMEGGWGDPNNIGPLGPTDYSDLRDFMNTYNSRPPESWWRANLNLRAY
                     YGYQAIVQAIHHYDIADGKNYFYHRDPQTGVWTVVPWDLDLTWADNMYRGGQTGGDEP
                     FKSRLLSNFSTNPAYPAIVREFRNRVREIRDLLWNSDEAFRLIDDYARLVRGTNAASI
                     IDADRAQWDYNPLMNNSSIVNLSKAGTGRFYQVGTPTRDFAGMVQLMKNYVGYRATNA
                     AFSLDTIADEPQRPARPTLTYIGPEGYPANRLRFRATPFNGSALAAIEWRAAEVSRAG
                     HPAFDPAQPGAYEITPVWESGPIADPSPEYALPQVAWRIGHLYRVRARYLDTAGRASN
                     WSPPIEFTPGPSDTADALVQHLCLSELMYNPIGGSDFEFIELHNRSLDFALDLGGATF
                     TAGVDFTFPSDTSLPPGGYLLLTRASTPEQQNAFRAFYQLDETV"
     gene            3614..3835
                     /locus_tag="PKK20_10410"
     CDS             3614..3835
                     /locus_tag="PKK20_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00340.1"
                     /translation="MERRRLKNHPALRQIGFAANRRVLQVEQLTHDCHIEAEAFDQLQ
                     KPAQVDGQHVSALPFGQERVQALFTVLVF"
     gene            complement(3839..>4544)
                     /locus_tag="PKK20_10415"
     CDS             complement(3839..>4544)
                     /locus_tag="PKK20_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV00341.1"
                     /translation="QKGELVADLVEHRQRFVLCRGGRGGLGNRNFATARRQAPRFAQP
                     GEPGESGEFRLELRLIADVGLVGFLNAGKSTLLTAISKARPRIAPYPFTTLTPKIGIV
                     EYPDWARLKVCDIPGLIEGAHRNLGLGHAFLRHIARCKVIVLVIDMAGTDGRQPWDDC
                     RQLLKELELHDPDLLNRPRLVVANKMDEPAAGPNLRSFKRRIPRTEVLRMAAAFDEGI
                     DEFKNRIRAAVQDASH"
BASE COUNT          817 a         1298 c         1578 g          851 t
ORIGIN      
        1 cacggtctcg tcgagttggt agaaggcgcg gaaggcgttc tgctgctcgg gtgtcgatgc
       61 gcgggtaagg aggaggtagc cgcccggggg gaggctggta tcgcttggaa aggtgaagtc
      121 gacgcccgcg gtgaacgtgg cgccacccag gtcgagcgcg aagtcgaggc tgcgattgtg
      181 cagttcgatg aactcgaaat cgctgccgcc gatcgggttg tacatcaact cggaaagaca
      241 caggtgctgc acgagcgcgt ccgcggtgtc ggatgggccg ggcgtgaact cgatgggagg
      301 cgaccagttg cttgctcgcc cggcggtatc gaggtagcgc gcgcgcaccc gataaagatg
      361 gccaatgcgc caggccacct gcggcagggc gtattcggga gagggatcgg cgatcggtcc
      421 actctcccaa accggggtga tttcataggc ccccggttgg gcggggtcga atgccgggtg
      481 accggcgcgg gaaacctcgg cggcgcgcca ctcgatggcc gctaacgcgc tgccgttgaa
      541 gggtgtcgcg cggaagcgga gccggttggc cggatagcct tcggggccga tgtatgtgag
      601 cgtggggcgg gcggggcgct gcggttcgtc ggctatggta tcgagcgaga aggcagcgtt
      661 ggtggcgcgg taaccgacgt agttcttcat gagttgcacc atgccagcga agtccctggt
      721 gggagtgccc acctggtaga aacgtcccgt gcccgccttg ctgaggttga cgatgctgct
      781 gttgttcatc agcgggttgt agtcccattg ggcgcggtcg gcgtcgatga tcgaagctgc
      841 gttcgtaccc cggaccaggc gcgcgtagtc gtcgatcagc cggaaggctt cgtcagaatt
      901 ccagagcaaa tcgcggattt cgcggacgcg attccggaat tcccgtacga ttgcgggata
      961 ggcgggattg gtggagaaat tggagaggag gcggctcttg aacggctcgt caccgcccgt
     1021 ctgtcccccc cggtacatgt tgtcggccca ggtcaagtcg aggtcccacg gaacgacggt
     1081 ccacaccccg gtctgggggt cgcggtgata gaagtagttt ttgccgtcgg cgatgtcgta
     1141 gtggtggatg gcctggacga tggcctggta accgtagtag gcgcgcaggt tgaggttggc
     1201 gcgccaccac gattcgggcg ggcggctgtt gtacgtgttc atgaaatccc gcagatcgga
     1261 atagtcggtc gggccgagtg ggccgatgtt gttgggatcg ccccagccgc cttccatctt
     1321 gtagaagttg ccgtcggcca gtccgtgctc gtcgagaaat cgcccgttgg gctgctcgat
     1381 ggccaggtag agcccccaga aatcgccgga gtactggtcg ttggggacgg tttcctgcgg
     1441 agcgtcgatg atgcggaact ggacgaacat cgtgtgcatg gacggcagac cgacgagttg
     1501 gaaaaggcgg aagccgacgc tttcgaagag gccctgctcg ccgcggtgca tataatcccc
     1561 ttgctggatg ttggcgccga gattgagctt ggtccaggcc gtcttcagtt ttcgtcccca
     1621 gttgtctcgc ggctcgaagt cgtgtccgcg attgaaattg aatttccaca tgttcttgcc
     1681 catggcatag cgccagacgc cccctcgggg gcggaagtgg atgtgatcgt agacgcggcc
     1741 gtcgtaaacg agggtgcctt gccagaagta ctcgtccccg tgcgaacggt cgtaccaggt
     1801 gcaatcctcg accgctttgc gcttggcaat caagtggtat accgggaggc ggttcatttc
     1861 ctcctcgccg acgatgaacg ttcgtccgag ggcgcccgcg gcgccgggac agacggcgcc
     1921 ggtccaggcg gggacgccgt catagcagaa atacgcgaag ttcggctgtg gatcgtcggg
     1981 atagggtgcg gaaacgctca cgcccgccaa atccgccgcc ttgaaccggt agcggaccag
     2041 gtggcgatgc tgttggagag atgcaggcag cgtggcggtg tagatgtcgt ctccggcctg
     2101 cgcatccccc tcgagaccgg cgtcgttcat cgacaccgcg gtccaagtcg acgcataaga
     2161 ctcgtccgac aattcgatgt agttgcccgg ggcgatcgct tggtattcga gggtcacatg
     2221 ggccagccct gaagcggagg tgacctttgc cgagatgcgg acagggcgac cggagccggg
     2281 ctggcgcggc tggtgttcga cctggcgaat ggcgggcggg atgcgcgcgg tgaaggtcgc
     2341 gttaagccgg ccgggcgttg gcccgattcc ggtggggccc acagtcgccc ggaggcggca
     2401 atcgaagaac gcgtcggaac tgtaccccag gttattgttg gccacctgca cagcaatcac
     2461 gttgtcaccc tcgcgcagca ggccgggggg cagggcaatc tcgtgccgat cataggcatt
     2521 gttctcgcgc gcgggattgg ccgcttgatt gtaaggcacc tcgccgttgg gcatggccag
     2581 gttcaggagg ttgttgccgt tgatccagag cttgaagccg tcgtcgtaga gcgcctcgag
     2641 ggagagcgag gtcaccgatg cggcgtcgac gacccggaac gtccggcgga tgaagaagtc
     2701 gaggtaaccg ctgcgcatgt cgtcgagggg tgttcccatg cgaacgctgg ggtcgtagcc
     2761 gaccggcgcc gggccgccga tccaggtgct gtcgttgaag gcaagatcgc gccagttggc
     2821 gggcgggggt tgcgttccct tgaaaacgcg ccactcggcg ccggcatcga acagcagcgt
     2881 gctctgttgc tggccgccgc cgacgaacga cgcgcgccaa tgcccgccca ggtcgttgtc
     2941 gatctccgga tgaatcagtt ccatcgagta gccgggcgcg tccccgacgg tgggccaggg
     3001 aaaaccgagt cgataatcga cgctgtcgac gatctggcgg gcggcgtcgc gcagctcaac
     3061 cttctcgcct tcaccggcca agcggccttt ccaggggccg agggcgtcga cgccgaactt
     3121 ggatttcaag gcggacgggt tttgagcgac gacgaggtag ccgccggcgg ggagcgtggt
     3181 gccgttgggg atgacaaagt cgattcccgc cgtgaacgcc cagcccgaga ggtcgacgtc
     3241 tgtggtgccg gagttgtaga gctcgatgaa ctcgaccggc tcggtcttca cgtcgggcga
     3301 gtgatggatc tcgttcataa ccacgtcggc ccggatcgac cagagggtca gttcgacagc
     3361 caggcaagcg ctcaacaagg ccaaacagtg ctgcatgaag ggcatcctag cagaaagcga
     3421 gccatggggc cagcggtcag agaatggacg ccgatcagcc ggagaccatc aggttcaacc
     3481 ccagaccaaa ccttcccatc cgtggctttc taaattccct tcttctttgc gtctctgcgg
     3541 ttcaatccga gacggtggct gtcatcggat gggattcacc acaaagtcgg ggagagcagg
     3601 gccaattcat caagtggagc ggcgacgcct caaaaaccac ccggcgctgc gccagattgg
     3661 ctttgccgcc aatcgacgcg tgctacaggt cgagcaattg actcacgact gccacatcga
     3721 agccgaggcg ttcgatcaac tccaaaaacc ggcccaggtc gacggccagc atgtctccgc
     3781 cttgcccttc gggcaggagc gggtgcaagc gctcttcacg gtgctggttt tttaaaactc
     3841 aatgactagc gtcttggacc gcggcgcgga tgcggttttt gaactcgtcg atgccctcat
     3901 caaacgccgc ggccatgcga agcacctcgg tgcgcgggat gcggcgtttg aacgagcgca
     3961 ggttggggcc ggcggcgggt tcgtccatct tgttggcaac caccaggcgc ggccggttca
     4021 acaggtccgg atcgtgcaat tcgagttctt tgagcagttg acgacagtcg tcccagggct
     4081 ggcgtccgtc cgtgccggcc atatcgatga ccagaacgat gaccttgcag cgcgcgatgt
     4141 ggcgcaggaa ggcatggccc agaccgaggt tccgatgcgc cccctcgatc aggccgggaa
     4201 tatcacagac cttgagtcga gcccaatccg ggtactcgac gatgccgatc ttcggggtga
     4261 gcgtggtaaa ggggtagggg gcgattctgg gccgcgcttt cgaaatggcg gtcaggagag
     4321 tcgacttgcc agcgttgaga aagccgacca agccgacatc agcgatcagt cgcagttcga
     4381 gtcggaactc gcccgattcg cctggttcgc cgggttgggc gaaccggggc gcctggcgac
     4441 gggcggtcgc gaaattccgg ttgcccaggc cgccgcgccc gccacgacac agcacgaaac
     4501 gctgtcgatg ctcgacgaga tcagcaacca gttctccttt ttgg
//
LOCUS       DAOQRO010000287         4530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_20284_length_4530_cov_5.211173, whole genome shotgun sequence.
ACCESSION   DAOQRO010000287 DAOQRO010000000
VERSION     DAOQRO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20284_length_4530_cov_5.211173"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            678..1181
                     /locus_tag="PKK20_10420"
     CDS             678..1181
                     /locus_tag="PKK20_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00342.1"
                     /translation="MTSFHSLFPELAMREVRCLHFGPGSALPAGEYIYLEFYCEDLAC
                     DCRRMFLQVISPTEPGRVFASINYGWEKESFYRKRMPYDPEAATGLVKGELDPINEQS
                     EFAELLLKGFQEFVLDEVYRLRVRRHHRLFREELARRAKGTARDSTGGNSATPEGGDA
                     GESERDL"
     gene            1556..3400
                     /gene="cas1"
                     /locus_tag="PKK20_10425"
     CDS             1556..3400
                     /gene="cas1"
                     /locus_tag="PKK20_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNV00343.1"
                     /translation="MLNEFVYCPRLFYYEFVEGVFVENADTLRGKGLHRRVDTGNGAL
                     PPAAGVGSKDKRSTSNAQRSTSKGESPAPTTATDDRGEPSTINREPSTDDVIHSRSVQ
                     MGSARLGATAKMDLIEVRTGGDDLFQALEVCPVDYKAGAPREGDDGNELWDADKMQLG
                     VQVLILRDNGYRCSEGVIYYRATKQRVRLPITPELEAWVVDTIARARAVATGSMPPPL
                     ANSPKCPRCSLVTVCLPDETRFLQTATAETAEEGRGRAPSPCPTPRRLIAARDDSRAL
                     YLTTQGLRVGRKGEVLQVKEERALLDEVRLKDVSHVALFGNIQISTQAVQALCEAEIP
                     VTYFSMGGWFYGITRGHELKNVFLRIEQFRRVGDDALALELSRQFVHGKIRNHRTMLM
                     RNHLEPPPGVITQLKQLADKALTATSREELLGLEGAAAAAYFAEFNGMLKVADELADA
                     RDPWDGQLRFNFEHRNRRPPTDPVNALLSLGYSLLAKDCTVAALTVGFDPYVGFYHQP
                     RFGRPALALDVMEEFRPLVAESVALACVNNRILSVKDFVRAGDAVNLTAAGRKRYFQA
                     YEQRLSTLLTHPLFDYKVSYRRALELQMRLLAKTLTGEIDEYVPLMTR"
     gene            3433..3636
                     /locus_tag="PKK20_10430"
     CDS             3433..3636
                     /locus_tag="PKK20_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00344.1"
                     /translation="MDHHIHTRDLSPAEQALVAELVADGLSIQNTFRPEPLCQKTGKG
                     HHESRTVEESQRARETTPGKAQP"
     gene            3959..>4530
                     /locus_tag="PKK20_10435"
     CDS             3959..>4530
                     /locus_tag="PKK20_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Cas7"
                     /protein_id="HNV00345.1"
                     /translation="MTNPEPLTLETLQQAVAGTAAAFRCRRRLQPGGGEGDKVFPPTF
                     AGAVYAVEQRRVPGRPDPVTCVVLDTVQSQANRMELALQEAVNAGRLQLPLIEVDFSP
                     WSPTGHKPEAERLIDDVGTITSLQVPHRLADAILRDSEIEEEADGKKVKLPFRKSSKG
                     RLLDTVSQRSATALYQLCPTALIFGMWDSSG"
BASE COUNT          872 a         1513 c         1398 g          747 t
ORIGIN      
        1 agggggtgga aggcttcctg gaggaggcgc tccagatcag cgcgccgcag gtcgcggtgc
       61 aattgcaggc cgaagcatta ggctatctct gattcccaca gaacggcgaa attatcgcca
      121 aaatgtcaag tccaagggac aactggaaga ggcaaaccgc cgcgctggta gctcggcttt
      181 gtcccaccga cgatcgcggc ggtccgcctc gacctttggg cggacgaaaa gcgcgcactt
      241 gaactcaggc aggcagcggg ccttgaacgc cctcgatgca tattcctgta ttccgggacc
      301 gaccccttta gggaccgacc cctttacgca ggcaaggagg cttggcttgg attcggatac
      361 ggccagcgca cccggccact tgcctgccag cgcctcggcg cacgcacgtc ggcgaagcgg
      421 attcgagtgg cgtgaacgat cgagttcttg tatgcatacg cacagtgtcg aagtttttga
      481 tttggcgccc tgttggcccg gcgccagcct ggcgcccgag catgcgccac gccgccctca
      541 ccggctgctc catcgccgtc aggcacacgc atagtctccg ctctccagcg gccggcgcgg
      601 tgaccgaatc cctgcgcccc gcctcctgac tctcaactct caactcatca cgctccactc
      661 atcactctcc actccgtatg acttcttttc attcgctgtt tcccgaactg gccatgcgcg
      721 aggtgcgttg cctgcacttc ggaccgggat cggcattgcc ggcgggcgag tacatctacc
      781 tggaattcta ttgtgaggat ctcgcttgcg attgccggcg gatgtttctt caggtgatct
      841 cgcccaccga acctggtcga gtgttcgcct cgatcaacta cggctgggaa aaagagtcgt
      901 tctatcggaa gcgcatgccc tatgatccgg aggctgcgac cggcctggtc aagggcgagc
      961 tggaccccat caacgagcag tctgagtttg ccgagcttct gctcaaggga ttccaggagt
     1021 tcgtcctgga cgaggtgtat cggctccggg tgcgacgaca ccaccggttg tttcgcgagg
     1081 aactggcacg ccgggcgaaa gggaccgcga gggattccac tggcggaaac agtgcaacac
     1141 cggagggggg cgatgccggc gagagcgagc gcgacttatg aaagcaaccc accataatca
     1201 gcctcaggca tccgcgggcg agggcaccgg cgcacgcagt cgaggcgcgt catgcacccc
     1261 gagcgatcag ccgacgcggt ccaggtgcga ggcagcagca ccaagccggc ctgcggcccg
     1321 tgacgcagtt gacgcggcgt gcgaacgccc gtcggggctg cccacgcccg aggaaatccg
     1381 ggagcgtgtg gtcgaagtca tgcggcagga gtatccgcca gccgaagcga acgggatcgc
     1441 cccagtcaat cggacggtcc aacagacggc ggccaccgac gccaatgctt cgctcccaac
     1501 tggccactcc gcgcgatcga ccgcggggac ggcgaccgac gcgatccccg cccgcatgct
     1561 caacgagttc gtctactgtc cgcggctgtt ctactacgag tttgtcgagg gcgtgttcgt
     1621 ggagaatgcg gacacgctgc gcggcaaggg gctgcatcgg cgggtggaca cgggcaacgg
     1681 tgcgctgccg ccggcggccg gtgtcgggtc caaggacaaa cgttcaacgt ccaacgccca
     1741 acgctcaaca tccaaaggcg aaagcccggc gccgacgacc gcgacggacg accgcggcga
     1801 accatcaact atcaaccgcg aaccatcaac cgacgacgtc attcattccc gctcggtgca
     1861 gatgggctcg gcccgcttgg gcgcgacggc gaagatggac ctgatcgagg ttcgtacagg
     1921 cggtgacgac ctcttccagg cgctcgaagt gtgcccggtc gactacaagg cgggcgcgcc
     1981 cagggaaggt gacgatggca acgagctgtg ggatgccgac aagatgcagc ttggtgtgca
     2041 ggtgttgatc ctccgcgaca acggctacag gtgttccgag ggcgtcatct attaccgcgc
     2101 aaccaagcag cgagtgcggc tgccgatcac gccggagctc gaggcctggg tggtcgacac
     2161 catcgcccgc gcccgcgcgg tcgccaccgg cagcatgccg ccccctttgg ccaattcgcc
     2221 caagtgcccg cgctgttcgc tggtcacagt ctgcctgccc gatgagacgc ggttccttca
     2281 gaccgcgacg gcggagaccg cggaggaggg ccgcgggcgg gctccatcgc cctgtcccac
     2341 gccgcgacgg ctgatcgcgg cgagggatga cagccgcgcg ctctacctga ccacccaggg
     2401 cctgcgcgtc gggcggaagg gcgaagtgct gcaggtcaag gaggagcggg ccctgctcga
     2461 cgaggtgcgg cttaaagacg tttcgcacgt ggcgctgttc gggaacatcc agatctcgac
     2521 gcaggccgtg caggctctgt gcgaagcgga gatcccggtc acgtacttct cgatgggcgg
     2581 ctggttctac ggcatcacac ggggacacga gttgaagaac gtcttcctgc gcatcgagca
     2641 gttccgtcgg gtcggcgacg acgccctcgc cctcgaactg agccgccagt tcgtccacgg
     2701 caagatccgc aatcaccgca ccatgctcat gcggaatcac ctcgaaccgc ctcccggcgt
     2761 gatcacccaa ctcaagcagc tcgcggacaa agcgctcaca gccaccagcc gtgaggaact
     2821 cctcggcctc gaaggcgccg ccgccgccgc gtactttgcg gagttcaacg ggatgctcaa
     2881 ggtcgcggat gaactggccg acgcgcgtga tccgtgggat ggccagttgc gcttcaactt
     2941 cgagcatcgg aaccggcgtc ccccgaccga tcccgtcaac gcgctgctgt cgctgggtta
     3001 cagtctcctg gcaaaggact gcaccgtcgc ggcgctgacg gtggggttcg atccgtacgt
     3061 cggtttctac caccaaccgc gttttggccg gcccgccctg gccctggatg tcatggagga
     3121 gttccgtccg cttgtggccg agtccgtcgc cctggcttgc gtcaacaacc gcatcctgag
     3181 tgtgaaggac ttcgtccggg ccggcgacgc ggtgaacctc accgccgccg gacgcaaacg
     3241 ctacttccag gcctacgaac agcgcctgag caccctgctc acccacccgc tgttcgacta
     3301 caaagtcagc taccgccgcg ccctcgaact ccagatgcga ctcctggcca aaaccctcac
     3361 cggcgaaatt gacgaatacg tgcccttgat gacccggtga accacggtgt acaattgcgc
     3421 gcatacaagc ctatggacca tcacatccac acccgggact tgtcaccggc ggaacaagcg
     3481 cttgtcgctg agttggtggc cgacggcctg agcatccaga acacgttccg cccggaaccg
     3541 ctttgccaga agaccggcaa gggccatcac gaaagcagaa ccgtcgagga aagccagcgc
     3601 gccagggaga ccacacccgg aaaggcacaa ccgtgaaacg cgcctggctg caacccttca
     3661 cctcgccgct ctcgatgccg tgagcaccaa gcgcctcctg cagcaggtcc gccgcaatgc
     3721 tggttttggc tcaccgccgg agacttcacg gcacccggcg ccctcatggc cgccacgaaa
     3781 aagtttcgac tctccccccg tatttgtccc accccctgtg ccagaattgc ccccaaataa
     3841 aacgaggcga agcgaggtcg aaaggcgccg ctccgctgcg cgggagcgtg ggtcttggag
     3901 actcacatcg acacccggct aacggccgct gcggtgccca acacccaagg aaccaactat
     3961 gaccaatcct gaacctctga ctctggaaac ccttcaacaa gccgtggccg gaacggccgc
     4021 ggcctttcgc tgccgtcgcc gtctccagcc tggtggtggc gagggtgaca aagtctttcc
     4081 acccaccttc gccggcgctg tctatgccgt ggagcaacga cgcgtgcccg gccgacccga
     4141 ccccgtgacg tgcgtcgtgc tcgacactgt ccagtcccag gccaaccgga tggaactggc
     4201 gttacaagag gcagtgaacg ccggacgact ccaacttccg ctcatcgagg tggacttcag
     4261 tccgtggtcg ccgacgggcc acaaaccgga ggccgagcgc ctgatcgatg acgtgggcac
     4321 catcaccagt ctccaggtgc ctcaccgcct ggcggatgcg atcctgcggg acagcgagat
     4381 tgaagaggaa gctgacggaa agaaggtgaa gctgccgttc cggaagagca gcaaaggcag
     4441 actgctcgac actgtcagtc agcggagcgc cacagcgcta taccaactct gcccgacggc
     4501 gctgattttc ggcatgtggg attcctccgg
//
LOCUS       DAOQRO010000288         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_20671_length_4473_cov_4.934812, whole genome shotgun sequence.
ACCESSION   DAOQRO010000288 DAOQRO010000000
VERSION     DAOQRO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20671_length_4473_cov_4.934812"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKK20_10440"
     CDS             complement(<1..455)
                     /locus_tag="PKK20_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNV00346.1"
                     /translation="MHHRIGLRQSSLFLLFLLVLSGSVVRGRVRAADGVWESVGVRCG
                     ISSHSSANRFIQTELWAAWNLTDLLDIGRKWSLQFQLDSSAGWLSGRGADAFVGGVGP
                     ALEIGRERLPISLRGGVSPTFLSRDSFGNKDFGILFQLTSHVGVEVHVLP"
     gene            complement(506..1576)
                     /locus_tag="PKK20_10445"
     CDS             complement(506..1576)
                     /locus_tag="PKK20_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNV00347.1"
                     /translation="MRDTSLEFLKTLVNTPSPSGHEARGQRVWLDYVGAYAQETFSDA
                     YGNCVAVLNKGGSPRLMLAAHADEIALTVNYIDKEGFLYVRKLGGVDAIVARAQRVVV
                     HTRNGPVRGVIGNVAPHLTKLDKEHKVPEIGDLFLDIGAADRKEAERLVQIGDPVTLS
                     DEFELLRNDLAVARAFDNRVGTFAVAEALRLLGANRRKLKAEVCAVSNIMEEVGLLGA
                     RQIAYSLKPDVALVVDVTHATDCPTVSRQLHGDIRIGKGPTLTHGGCNHPEVIARLEQ
                     VAKKKNIKLQHEAMSASSGTDTDVIFWTRGGIASGLISLPNRYMHSPVELVSLKDLEK
                     IPELMAAFALSLERGEQFKVRI"
     gene            complement(1864..2910)
                     /locus_tag="PKK20_10450"
     CDS             complement(1864..2910)
                     /locus_tag="PKK20_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNV00348.1"
                     /translation="MNTTREIRRHFASDNYGGICPEAWAALAEANSGHAVSYGEDAWT
                     AQAADLIRDVFEMACEVFFVFNGTAANSLSIASLGHSYHSILCHEIAHVETDECGAPE
                     FFSNGMKVLLMPGSNGKLDPCAIERMVDKRADIHYPKPRAVSVTQATEVGTVYSVEEL
                     KSIWAKTKQCGLKLHMDGARFANAVASLGVKPKEITWQTGVDVLCLGGTKNGMHVSEA
                     VVFFNRELAAEFDYRCKQAGQLYSKMRFMAAPWVGMLKDGAWLRHAAHANAMAELLET
                     ELAQLPEARILFPRQANSLFVELPPSVIEGLHKAGWRFYTFIGQGGCRFMCAWDIREE
                     DVRALVADIKRLIG"
     gene            complement(3338..4372)
                     /locus_tag="PKK20_10455"
     CDS             complement(3338..4372)
                     /locus_tag="PKK20_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00349.1"
                     /translation="MIWNPLEQRFLLVGLLLLLGLGCAAPRPVTPRVDSPPAPFIRAA
                     HRSDGTAALEVALRAFKPARGRGPTLWLVGVTHLGTTDYYGRLQRFLNAQDLVLFEGV
                     GATNKSFALQREAGFNLQAALAKALGLEFQLHAIDYTPRHFQNSDLSVAELARIFANS
                     SPGTQDTPSPQGASGSEPRSGERGGAEFDHLLAAMQGTGFLGAMARVTVALLEASPRL
                     QAAMQVVLIEVLSGLPNDLPQMPGLPPGFQNLLRVLIEERNEKVIEDVRRALEAKDPP
                     RSIAIFYGAGHMTDLEERLRSTLEYRPAVERWMTALAVNPERSALNARQLDFVRSWVR
                     GRMGELQRAP"
BASE COUNT          852 a         1401 c         1386 g          834 t
ORIGIN      
        1 ggcagcacat ggacttcgac tccaacgtgg cttgtcagtt ggaacaggat accaaagtcc
       61 ttgttgccaa atgaatcgcg ggacaggaac gtcgggctga cgcccccgcg caaagagatc
      121 ggcaggcgct cgcgtccgat ctccagcgct ggtcccaccc cgcccacaaa agcatccgca
      181 ccccgcccgc tgagccagcc ggcggaactg tccaactgga attgcaggga ccacttgcgt
      241 ccgatgtcca ggaggtccgt gagattccag gcggcccaca gttcggtttg gatgaagcgg
      301 tttgccgacg agtgcgacga gatgccgcaa cgcacgccca cggattccca aaccccatcg
      361 gctgcccgaa cgcgacctcg aacgacggac ccgctgagaa caagcaggaa gaggagaaac
      421 agcgacgatt gccgcagacc gatccgatgg tgcatggaca tctgaaggct caattcagcc
      481 cggtcgatcc ggggggaaag ggggctcaga tgcggacctt gaactgttcc ccgcgttcga
      541 gcgacagcgc gaaagcggcc atgagttcgg ggattttctc gagatccttg aggctgacga
      601 gttcgacggg agaatgcatg taccggttgg gcaggctgat gagcccactg gcgatgccgc
      661 cgcgcgtcca gaagatgacg tcggtgtccg ttccgctgct ggcggacatg gcctcgtgct
      721 ggagttttat gttcttcttt ttggcgacct gttcgagccg ggcgatcacc tcgggatggt
      781 tgcatccccc gtgggtcagg gtgggtcctt tgccaatccg gatgtcgccg tggagttgcc
      841 ggctgacggt cgggcagtcg gtcgcatgag tgacgtcgac aaccagtgcc acatccggct
      901 tcagcgaata cgcgatctgg cgcgcgccca ggagccccac ctcctccatg atgttcgaca
      961 ccgcgcacac ttccgctttg agcttgcggc gattcgcccc aaggagtcgg agcgcctcgg
     1021 ccaccgcgaa agtcccgacc cggttgtcga acgcgcgggc gacagccaag tcgttgcgca
     1081 gcaattcgaa ttcgtcgctc agtgtgacag ggtcgccaat ctgcaccaac cgctccgcct
     1141 ccttgcgatc cgccgcgccg atgtccagaa acaggtcgcc gatctccggc accttgtgtt
     1201 ccttgtccag cttggtcagg tgcggcgcga cgttgccgat gacgccccgg accggaccgt
     1261 tgcgggtatg gacgacgacg cgctgggcgc gcgcgacaat cgcatctacg cccccgagct
     1321 tacgaacgta gaggaatcct tccttatcga tgtagttgac ggtcagcgcg atttcgtcgg
     1381 catgcgcggc gagcatgagg cgtggcgaac cgcccttgtt caagacggcc acgcagttgc
     1441 cgtaggcgtc cgagaacgtc tcctgggcgt aagcgcccac gtagtcgagc cagacgcgtt
     1501 gtccgcgcgc ctcatgaccc gacggactcg gcgtgttcac gagtgtcttc aggaattcaa
     1561 gcgaggtgtc gcgcatggat cgagtatggg caaaccaggc tccgttgcca agcccgcgct
     1621 gattgccgca tgaacggtca gcacatatcg gagtcagcgc gcactcatcg cacacttcat
     1681 cgcgcgcttc atcgcacact ggatcgatct gtggcgagcg gcatgaagcg gcaaggatgt
     1741 tctccctgga caaatcgatg gtgcacgatc aaggccctgc agccacatcg acaagcatcg
     1801 agaatgccca aacgccagcc gtcgtcgtca gacggcgctg ttgcctgcat ttgacggggc
     1861 atctcatccg atcaggcgtt tgatgtctgc gaccagggcc cggacatctt cctcgcggat
     1921 gtcccaggcg cacatgaatc gacatccccc ctgcccgatg aacgtgtaga atctccagcc
     1981 ggctttgtgg aggccctcga tcacggacgg cggcagttcg acaaagagcg aattcgcctg
     2041 acgcggaaaa aggatgcggg cttcgggcag ttgggccagt tcggtctcga gcaattcggc
     2101 catggcattg gcgtgggccg cgtggcgcag ccatgcgccg tccttgagca tcccgaccca
     2161 cggggccgcc atgaagcgca tcttcgaata gagctggccc gcctgtttgc atcggtaatc
     2221 aaactccgcc gccagttcgc ggttgaagaa gaccaccgcc tcgctgacat gcatgccgtt
     2281 cttcgtcccg cccagacaga gaacatccac gccggtctgc caggtgattt ccttcggctt
     2341 caccccgagc gacgccacgg cgttggcgaa gcgcgccccg tccatgtgca gcttgaggcc
     2401 gcactgtttt gtcttggccc aaatggactt gagttcctcg acgctgtaga cagtcccgac
     2461 ttcggtcgcc tgcgtcacac tcacggcccg gggcttgggg tagtgaatat cggcgcgctt
     2521 gtcgaccatg cgctcgatgg cgcacggatc gagcttgccg ttcgagcccg gcatgagcag
     2581 gaccttcatt ccgttcgaga agaactccgg cgcgccgcat tcgtcggttt cgacgtgtgc
     2641 gatttcgtgg cagagaatgc tgtggtatga atggccgagc gaggcgatcg agagggagtt
     2701 ggcggccgtg ccgttgaaaa cgaagaacac ctcgcaggcc atctcgaaca catcacggat
     2761 caggtcggcc gcctgggccg tccaggcatc ttcgccgtag ctgacggcgt ggccgctgtt
     2821 ggcctcggcg agggccgccc aggcctcggg acagatgcct ccgtaattgt cgcttgcaaa
     2881 gtgacgtcgg atctctcggg tcgtattcat cctcaaaaag ccgcttctct aacatcgggc
     2941 tctgcccgct ggcgagcgga acgttgaaag gtcagaggtc agaggtggac gtcgagcgtt
     3001 gagcgttgaa cgtttttggt tcatggctcc cttgcgcatt caagctctgc atgccgcttg
     3061 ccgtgaatca cccgtgcgat tctggcacac caattcaaat gagaatgggg ttcgcaacaa
     3121 caccgtcttg gccgcgacct ttgtcggctt cgtcggtcgc cacgctgtct gacgacaatg
     3181 gtcccgacga aggcggaatc ggccaccagc aaccagcagg ccgccgtccg cagattcagc
     3241 accgtgacga atgccacaca gaaactgtcc ggacgaaagc tcgaatacaa cagcccggat
     3301 atcggcgtcg tcgtcgcccc agaaggtttc ggtatggcta cggggcgcgc tgcaactcgc
     3361 ccatgcggcc gcggacccag gaccgcacaa agtcgagttg acgcgcgttg agcgctgagc
     3421 gctcggggtt gaccgccaag gccgtcatcc agcgctcgac ggcggggcga tactcgaggg
     3481 tggaccgcag gcgttcttcg aggtccgtca tgtgccccgc accgtagaag attgcgatcg
     3541 accgcggggg gtctttggct tcgagagcgc ggcgcacatc ctcgatcacc ttctcattcc
     3601 gctcttcgat gaggaccctg agcagattct gaaagcccgg aggcaagccg ggcatctgtg
     3661 gcagatcgtt cggcagaccc gagagaacct cgatgagcac gacttgcatg gccgcctgga
     3721 gccgcggact ggcctcgagc agcgcaacgg tgacccgcgc catcgcgccg agaaagccgg
     3781 tcccctgcat cgccgccagc agatggtcaa actcggcgcc gccccgttca ccggatcgag
     3841 gttcgctgcc tgacgcgcct tgcgggcttg gggtgtcttg cgtgcctggc gaggaattgg
     3901 caaaaatgcg cgccagttcc gccacggaca ggtcgctgtt ttggaaatgc cgcggggtgt
     3961 aatcgatcgc gtgcagttgg aattcgagcc ccaaggcctt ggccagcgcg gcctggaggt
     4021 tgaagccggc ctcacgctga agcgcgaacg acttgtttgt cgcccccaca ccctcgaaca
     4081 gcaccaaatc ctgtgcgttg aggaaacgct gaagcctccc ataatagtcc gttgtcccga
     4141 gatgcgtcac acccacgagc cagagcgtcg ggccgcgtcc gcgcgccggt ttgaaggccc
     4201 tcaaagcaac ctcgagcgcc gcagtgccat cggagcgatg ggccgcccga atgaatggcg
     4261 cgggaggcga gtcaacccgc ggcgtgactg ggcgcggcgc ggcacaaccc agacccagca
     4321 gcaatagcag gccaaccagc aggaaccttt gttcgagagg attccagatc acgcccgatg
     4381 ctgatcatcc cgacgtcgcc tggcgagtcc aaaggttcct agtgtagtgt ctcacaaacg
     4441 actggagtta ttttcccttt gtttttcgca ttc
//
LOCUS       DAOQRO010000289         4442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_20910_length_4442_cov_4.450878, whole genome shotgun sequence.
ACCESSION   DAOQRO010000289 DAOQRO010000000
VERSION     DAOQRO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20910_length_4442_cov_4.450878"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4429)
                     /locus_tag="PKK20_10460"
     CDS             complement(<1..4429)
                     /locus_tag="PKK20_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNV00350.1"
                     /translation="MNLNENTVSVLLGDGLGAFAAPAKYPTPNGPYAVAVGDFDGDGH
                     LDLAVANHSGSTVSLLKGAGDGTFGPRVDYASGGSNPYHVVAADLNADGLPDLLASNY
                     GNSQLGVIFNKGDGTFGAPVSYPAGGNAISSVAGDWNNDGRMDIATANFGNNTVSVFL
                     GNDTEWLAFDPVGHGVRVAAGQGNLSNTGDYDYWSFSAKAGERLVVGVEVPGRPNASQ
                     LYYLIEAIDGQDLQGFYPDYRGWGQSGMITIPRDGTYLVRVSYNHDYQGEYQIRVVLT
                     PPEVAQEDEENGSVGSASALALTGGGGTRSGAAVGYIRTAGDLDYFDLGTLESGQTVF
                     LNARLFAGSTLAPVVSLHDAANGYVVEAPGGRPFDSVAEVRINTTGRYYAVVRGGANQ
                     GGLLQWYVLETQVMPTGSVAFPNLQVTSLLPPSGSGLESGDPVAVSFTVKNVGGLGTV
                     GATWTDRLVISPAAVYGDLNDIELAVFPHSGALESLDEYTVSQTVSLPEGIEGEFYTI
                     VMTDAGNAIAEFILEADNVTVSDTTFAVTRAAYSDLRVENLSAGLAGGSIEVEWTTAN
                     RGDGAARGGVVESVLLRNVVTGVVEYRTELAIAGDLGPGETAARSATLPLPAAGGYEV
                     RVVTDSDDALFEFDAFSHASAEGNNQAAVPLDVVVDLVVVSLRVEPAGALFSGQNVTI
                     AWQDSNLGNRPTSGSWRDRIVVRNTTSGQTLHDGTIQHNAQADGDLGPGASFARSYAF
                     QLPHLQPGVGNIEFKVTCDEANQIAEFNPQATAEANNASAVTRASTLAPYPDLAVTAI
                     VTPERADADQPFEVTYTVVNQGDAATSSAWSDHLYWSTRVDGTDLHYLTAVPQTSALA
                     ANGGSIEQTVEVSLPAGPSGSRYILVRTDALNQVGEKSEDLNDRYSDVIDLDVPNLTV
                     TVTGLPAAGEGGQDVEVEWTVRNEGDGDTFAASWRDRLWLGTAPDGSGALDVLRDVEH
                     TDTLEAGASVVRTATVTLPAGLPGNRYLVVRTDVGGAIGESDETDNTAVSDTPLDLRA
                     PDLEVISAAAPATAQFGQVIPLMFRLRNAGNGSANGAWHDAIYFSRDGVPAGANNVLL
                     AARPSGDASPLGPGASTANLTVNVTLPATGFADGTHYLLVRTDGYGVVTESNENNNWS
                     APVPITLTAQARPNLVVEDIAVPATAAPGEAFTVTWKTRNAGAADLTGSWSETVALST
                     DNLIGNDQTFATVDFLGTLAMGAVVERSAQVTIPLDGPAGLLYAVVTTDRGGNVIESN
                     EADNTALGMTPVNVPLRLRLTASSARVPESANTLGFTVSRNGSRDAALTVNLSSSDTT
                     ELTVPPTVEIASGQAAAYFNGTPQKDGVADGDQLVALNAKASGFADAAATVTVTDVNQ
                     PVLTLILPATLGEGQTANATVKRDAALPTPLMVTIGSSSPSQLTAPASVTIPADEDQA
                     TFRIAAVEDTQIESDTGYELTVSAVGAVGDTKSVKVIDND"
BASE COUNT          744 a         1537 c         1369 g          792 t
ORIGIN      
        1 ggtcgttgtc gatcaccttg acgctctttg tgtcgcccac cgcgcccacc gcgcttacgg
       61 taagctcata cccggtgtcg ctctcgatct gcgtgtcctc gaccgccgca atccggaacg
      121 tcgcctgatc ctcgtctgct gggatcgtaa cgctggcggg ggcggtgagt tgagacggac
      181 tcgagctccc gatggtcacc atcagcggcg tgggcagagc ggcgtcacgc ttgaccgtcg
      241 cgttggccgt ctggccttcg cccagagtgg ccggcaggat cagggtcaac accggctggt
      301 tgacatccgt cacggtcaca gtcgctgccg cgtcggcaaa cccgctcgcc ttcgcgttca
      361 acgccaccaa ctgatcgcca tccgccacgc cgtccttctg cggggtccca ttgaaatagg
      421 ccgccgcctg cccactcgcg atttccactg tcggcggcac cgtcaactcg gtcgtgtcgc
      481 tgctgctcag gttcacggtc agagcggcgt cgcggctccc gttgcggctg acggtgaacc
      541 cgagcgtgtt ggcgctctcg ggcacccggg ccgagctggc tgtcaaacgc aggcgcagcg
      601 gcacgttcac cggcgtcatc cccagcgccg tgttgtcagc ctcgttcgac tcgatcacgt
      661 tcccgccgcg atccgtcgtc accaccgcgt acagcaggcc ggcaggccca tccagcggaa
      721 tggtgacctg ggcggaccgc tccaccacgg cgcccatcgc cagcgtgccg aggaaatcca
      781 cggtggcaaa cgtctgatcg ttgccgatga gattgtccgt cgacagcgca acggtttccg
      841 accatgagcc ggtcaggtcg gctgcgcccg cgttccgagt cttccacgtg acagtgaatg
      901 cctcgccggg agccgcggtc gccggcaccg cgatgtcttc gacaaccagg ttcgggcgcg
      961 cctgcgcagt cagcgtgatc gggacgggcg cgctccagtt gttgttctcg ttcgactcgg
     1021 tcacaacccc gtacccgtcg gtgcgcacga gcagataatg cgtgccgtcg gcgaacccgg
     1081 tcgccggcaa cgtcacgttc acggtcagat tcgcggtgct ggcgcccggc cccagcggcg
     1141 aggcgtcgcc cgacgggcgc gcggccagga gaacattgtt ggcgccggcg gggaccccgt
     1201 cgcgggagaa gtagatcgca tcgtgccatg ccccattggc ggatccattg cccgcattgc
     1261 ggagtcggaa catgagcgga atcacctggc cgaactgggc cgtggcgggt gccgccgcgc
     1321 tgatcacctc gaggtcgggc gccctcagat cgagcggcgt gtcgctcacg gcggtattgt
     1381 ctgtttcatc ggactcgccg atggcgccgc cgacgtctgt tcttaccacc agataacgat
     1441 tgcccggcag accggccggc agcgtcacag tggcggtgcg cacaacactc gcccccgcct
     1501 cgagcgtgtc ggtgtgctcg acgtctcgaa ggacgtcgag tgcgccgctg ccgtccggag
     1561 ccgtcccgag ccagagccga tcgcgccacg atgcggcgaa cgtgtcgccg tctccctcgt
     1621 tccgaaccgt ccattcgact tcaacatcct ggccgccctc gcccgccgcc ggcaaacccg
     1681 tcacggtcac tgtcagattg ggcacgtcga ggtcgatgac gtcgctgtag cggtcattga
     1741 ggtcttcaga cttctcaccc acctggttca aagcgtcggt tcgcaccagg atgtaccggg
     1801 aaccgctggg accggcgggg aggctgacct cgaccgtctg ctcgatcgaa ccgccgttgg
     1861 cagccaatgc ggacgtctgc ggcacggcgg tcagatagtg caggtcggtg ccgtcgaccc
     1921 tcgtgctcca atacaagtga tcagaccatg cgctggaagt ggcggcgtcg ccctggttca
     1981 ccacggtata ggtcacctcg aacggctgat ccgcatcagc gcgttcgggc gtcacgatgg
     2041 ccgtcacggc gaggtccgga taaggcgcaa gagtcgaggc gcgcgtgacg gcggaggcgt
     2101 tgttggcctc cgcggtggcc tgcgggttga actcggcgat ctggttcgcc tcgtcgcatg
     2161 tcaccttgaa ttcaatgttc ccaacccccg gctgcaagtg cggcaactgg aaggcgtagg
     2221 atcgtgcgaa cgaggcgccg ggtccgaggt cgccgtcggc ctgggcgttg tgttggatcg
     2281 tgccatcgtg caacgtctgt ccgctggtgg tgttgcgcac gacaatccga tcgcgccacg
     2341 aacctgaggt ggggcgattt cccaagttgc tgtcttgcca ggcaatggtc acattctggc
     2401 ccgagaacaa cgcccccgcc ggttcgacgc gaagactcac aaccacgaga tccaccacca
     2461 catcgagcgg caccgccgcc tgattgttgc cctccgcgct ggcatggctg aaggcgtcga
     2521 actcgaacaa agcgtcgtcc gaatcggtta cgacacgcac ttcgtaaccg ccagccgcgg
     2581 gcaatggcag ggttgcggac cgcgccgcgg tctcgcctgg accgaggtcg ccggcgatcg
     2641 ccagctccgt ccgatattcg acaacccccg tcacgacatt gcgcaacagc acggattcca
     2701 caaccccccc tcgagcggcc ccgtctccgc ggttggcggt cgtccattcc acctcgatgc
     2761 tacctcccgc gagccccgcg ctcagattct cgaccctgag gtcggaatag gccgctcgag
     2821 tgactgcgaa ggtggtgtcg gagacggtca cgttgtccgc ctcgaggatg aactcggcga
     2881 tggcgttgcc ggcgtcggtc atgacgatgg tgtagaactc gccttcgatg ccctccggca
     2941 ggctcaccgt ctggctgacg gtgtactcat cgagcgattc gagcgcgccg ctatgcggga
     3001 acaccgccaa ctcaatgtca ttcaggtcac cgtaaacagc agcaggcgaa atcaccaacc
     3061 ggtcggtcca ggtggcgccc acggtcccca atccacccac gttcttcacg gtgaaagaaa
     3121 cagccaccgg atcgccgctt tcgagaccgc tgcccgacgg cggcagaaga ctggtcacct
     3181 gcaggttcgg gaaagcgacg ctgcccgtgg gcatcacctg cgtttcgagc acgtaccact
     3241 gcagcagacc gccctggttc gccccgcctc gaacaacagc atagtagcgg cccgtggtgt
     3301 tgatacgcac ctccgcaacc gaatcgaagg ggcgtccgcc cggtgcttcg acgacgtagc
     3361 cgttggctgc atcgtggagg ctcaccacag gcgccaaggt gctgccagcg aagagccggg
     3421 cgttcaggaa caccgtctgg ccgctctcca aagtgcccaa gtcgaagtag tccagatctc
     3481 ccgccgtccg gatgtaaccg accgcggcac cgctcctggt gcccccgccg ccggtcaacg
     3541 ccagcgccga cgcactcccg accgagccgt tctcctcgtc ctcttgcgcc acctccggcg
     3601 gcgtgagcac cacccgaatc tggtattcgc cctggtaatc gtggttgtag ctcacccgca
     3661 caagataggt gccgtcccgc ggaatggtga tcatacccga ttgcccccag ccgcgatagt
     3721 ccgggtagaa accctggagg tcctggccgt cgatggcttc gataaggtag tacaactggc
     3781 tggcgttggg ccggcccggc acctccaccc ccacgaccaa ccgctcaccc gcttttgcgc
     3841 tgaagctcca gtaatcgtaa tcgccggtgt tgctcaggtt gccttgaccc gccgcaaccc
     3901 gcacgccatg ccccactgga tcgaaggcca gccattcggt gtcgttgcca agaaacacgc
     3961 tgaccgtgtt gttgccgaag ttcgccgtgg cgatgtccat ccgcccgtcg ttgttccaat
     4021 cgcccgccac cgaactgatc gcgttgccgc ccgccgggta gctcaccggg gcgccgaacg
     4081 ttccgtcgcc cttgttgaag atcacaccca gttggctgtt cccgtagttg gacgcaagca
     4141 aatcgggcag gccgtccgcg ttcagatcag ccgcgacgac gtggtacgga ttcgatccgc
     4201 cgctggcata atcgaccctc ggcccaaagg tcccgtcacc cgcacccttg agcagactga
     4261 cggtcgagcc cgagtgattg gccaccgcca ggtccaaatg cccgtcgccg tcgaaatcgc
     4321 ccacagccac cgcgtaaggg ccgttcggcg tgggatactt cgccggagcc gcaaaagcgc
     4381 ccaggccgtc gcccagcagc acgctcacgg tgttctcgtt gagattcaca acggcgaggt
     4441 cc
//
LOCUS       DAOQRO010000290         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_20996_length_4428_cov_3.657672, whole genome shotgun sequence.
ACCESSION   DAOQRO010000290 DAOQRO010000000
VERSION     DAOQRO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20996_length_4428_cov_3.657672"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PKK20_10465"
     CDS             <1..1261
                     /locus_tag="PKK20_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00351.1"
                     /translation="KPLAIFVASETVDLWQSGRREHKMQSKLRTHYEVALDMHRLYAV
                     VYEWIKGIDAHQAFKDGIISRETMKGLALDAQQVMDDAGFKVCDNKPDHIIVRPRSDH
                     ELARTPKGQTVYALVDFELLQRTVSNEERVRQLKRLDYLKRQRDRFNVSRAENPYPNL
                     NLVTLLGVDYVYGRVESVRGRLWVVGKDPVLYDYFLPERWRHFPKTQISVYSDMYQTI
                     TKDGIHLVLQASRVGQQPDMDPFREDEQRILEHGYNSPFEEVSLAVDLAKRGVACIYP
                     RAIFMSDSTAEIGRTVFDGSRYSSHEGLLTPDGEPILKPHRDYIIIWGYWNGPDEKLA
                     DRDGDYYEGIHALRAYKEGLIDRPTYFALLEQARRKLGDVGVEDLNLRGNHILVSRDS
                     RSRLVVDDRGFPEIRLSNFEFLKRVKS"
     gene            1265..3067
                     /locus_tag="PKK20_10470"
     CDS             1265..3067
                     /locus_tag="PKK20_10470"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNV00352.1"
                     /translation="MKDTKTDHKRLLAWVDEVKSLCLPDRVVWTDGSKDEYNRLLADA
                     VRTGAATRLNARKRPNSYLFRSDPSDVARLENRTFIATRNEKDAGPTNNWIDPGELKS
                     TMRQLYAGCMRGRTMYVIPFSMGPIGSPIAKTGVEITDSPYVVVNMHIMTRVGQKVLE
                     ALGSDGEFIPCLHSIGAPLSPGEPDRPWPCAPIEQKYIAHFPEESLIWSYGSGYGGNA
                     LLGKKCLALRIASHLAHSEGWLAEHMLILRLTNPQGRRFHIAAAFPSACGKTNLAMLV
                     PTIPGWKAECIGDDIAWMRIGPDGRIRAINPEAGFFGVAPGTSNKSNPMAMRSIERNT
                     IFTNCALTDDGDIWWEGMDQACEHAIDWRGNDWTPDSDRLAAHANARFTAPASQCPVI
                     CPDWEDPAGVAIDMFIFGGRRSTTIPLVHQAFNFDHGVFLGASAASETTAAALDVGRT
                     LRRDPFAMTPFIGYHAGDYIAHWFRMGERLGAKAPAVFFVNWYRRDRSGRWLWPGFGE
                     NSRVLKWMCERVEGKIDAVRTPIGWMPRNGDLDVNGLTIRSDDFAELMRVSVEPLRKD
                     LSDAEQYFAQFGDRLPDKLRVELDAMRQRLAAAG"
     gene            complement(3117..3539)
                     /locus_tag="PKK20_10475"
     CDS             complement(3117..3539)
                     /locus_tag="PKK20_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00353.1"
                     /translation="MNLPATTRTPWCLILLLTGCVPTAPSYRPQPPIEAVTNAVAGPS
                     QSGATRPAPGPAPTRYPIVTVIERSIGKVTGVNTQAMFVVVDFGFNPLPRPEQRLTVF
                     RTNQAVGTVRMTGAPNGSFMAGDIMSGEARTGDEVRDE"
     gene            complement(3536..4309)
                     /locus_tag="PKK20_10480"
     CDS             complement(3536..4309)
                     /locus_tag="PKK20_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00354.1"
                     /translation="MKRSINWLAFLSLTGTLACIASETNTVDTAAFRARAERIYSAAG
                     ERRDRESSDPSAAWHFARACYDWADFATNDTQRAAIAQEGIAASRSVLRRHPRLAPAR
                     YYLAMNLGQLARTRLLGALSLVSEMEKHFKEVLDLDPDFDHAGPDRCLGLLYRDAPGW
                     PLSLGSKSKARAHLLRAVQRCPDSVENRLNLIETLLAWSEETEAARHYTDTAALLPTA
                     RTRYAGEEWAASWADWDCRWAAICQKLESRQFISRSPPQ"
BASE COUNT          825 a         1366 c         1441 g          796 t
ORIGIN      
        1 gaagccgctg gcgatcttcg tggcctccga gactgtggac ctgtggcaga gcggtcgcag
       61 ggaacacaag atgcagtcca aactgcgcac gcactacgag gtggcgctcg acatgcaccg
      121 cctctacgca gtggtgtacg aatggatcaa gggcatcgat gcccaccagg cgttcaagga
      181 cggcattatc agccgcgaga cgatgaaggg gctggccctg gacgcccagc aggtcatgga
      241 cgacgccggg ttcaaggtgt gcgacaacaa gcccgatcac attatcgtcc ggccgagatc
      301 ggaccacgag ctggcgcgca cgcccaaggg gcagaccgtg tatgcccttg tcgatttcga
      361 gctgctccag cgcaccgtgt cgaacgagga gcgcgtgcgg cagctcaaac gcctcgatta
      421 cctcaaacgc cagcgtgacc gcttcaacgt gagccgcgcc gagaatcctt atcccaacct
      481 caatctcgtg acgcttctgg gtgtggatta cgtttatggc cgcgtggaaa gcgttcgggg
      541 ccggctctgg gtggtgggca aggaccccgt cctttacgac tatttcctgc ccgagcgctg
      601 gcgccatttc ccgaagaccc agatttcggt ctacagcgac atgtaccaga cgatcaccaa
      661 ggacggcatc catctggtct tgcaggcctc gcgcgtgggc cagcagcccg acatggaccc
      721 ctttcgcgag gacgagcaga gaatcctcga gcacgggtac aacagtcctt tcgaggaggt
      781 gtcgttggcg gtcgatcttg ccaagcgggg agtggcctgc atctatccgc gtgccatttt
      841 catgtcggac tcgacggccg agatcggccg cactgtgttc gacgggtcgc gctacagctc
      901 ccatgagggt ctgctcacac ccgacggcga gccgatcctc aagccccacc gcgattacat
      961 catcatctgg ggctattgga acgggccgga cgagaagctg gcggacagag acggcgatta
     1021 ttacgagggc atccatgcgc tccgcgccta caaggagggg ctcatcgaca ggccaaccta
     1081 ctttgcgctg ctcgaacagg cgcggcggaa gttgggcgac gtcggtgtcg aggacctcaa
     1141 cctgcgaggc aatcacatcc tggtttcccg cgatagccgc agtcggctgg tggtcgacga
     1201 tcggggtttt cccgagattc gcctcagcaa tttcgagttt ctcaagagag tgaaatcctg
     1261 acacatgaag gacaccaaga cagaccacaa gagacttctc gcctgggtcg acgaggtaaa
     1321 gagcctgtgc cttcccgacc gggtcgtatg gaccgatggt tcgaaagacg agtacaaccg
     1381 tctgctcgca gacgcggttc gaaccggagc cgccactcgc ctgaatgcgc gcaagcgccc
     1441 gaacagctac ctgttccgat ccgatccatc ggacgtggcg cgcctcgaga accggacctt
     1501 catcgccacg cgcaacgaga aggacgccgg tccgacgaac aactggattg accctggcga
     1561 gctgaagagc acgatgcgcc agctttacgc cggctgcatg cggggccgca cgatgtatgt
     1621 gatccccttc tcgatggggc cgatcggttc tcccattgcc aagacaggcg tcgagatcac
     1681 cgattcccct tacgtggtgg tgaacatgca catcatgacc cgcgtgggcc agaaggtgct
     1741 cgaagccctg ggatcggacg gcgagttcat cccctgcctt cactcgatcg gcgcgccgct
     1801 ctcgccgggc gagcccgaca ggccctggcc ctgcgcgccc atcgagcaga agtacattgc
     1861 gcactttccg gaggagagcc tcatctggtc ttatggttcc ggatacggcg gcaacgcgct
     1921 gctgggcaag aagtgccttg ccctacgcat cgcctcccat ctggcccaca gcgagggatg
     1981 gctcgccgag cacatgctga tcttgcggct caccaatccc cagggtcggc ggttccacat
     2041 cgccgccgct tttccttcgg cctgtggcaa gaccaacctg gcgatgttgg tcccgacgat
     2101 ccccggttgg aaggccgagt gcatcggcga cgacatcgcc tggatgcgga tcggacccga
     2161 cggtcggatt cgcgccatca atccggaagc cggcttcttc ggcgtcgcgc caggcacctc
     2221 gaacaaatcc aatcccatgg ccatgcgctc gatcgagcgg aatacgattt tcaccaactg
     2281 cgccctgact gacgacggcg acatctggtg ggaaggaatg gatcaggcgt gcgagcatgc
     2341 catcgattgg cggggtaacg attggactcc cgactccgac cggttggccg cgcatgccaa
     2401 cgcccgcttt accgcgcccg cctcgcaatg ccctgtgatc tgcccggact gggaggatcc
     2461 agccggtgtg gcgatcgaca tgttcatctt tggcggacgg cgctcgacca cgatccccct
     2521 ggtccatcag gcgttcaact ttgatcatgg cgtcttcctg ggggcctccg cggcgtcgga
     2581 aaccacggcc gccgctctcg atgtcggtag aacgctccgt cgcgatccgt tcgccatgac
     2641 cccgttcatc ggataccacg cgggcgacta catcgcgcac tggtttcgga tgggcgagcg
     2701 gttgggagcc aaggcgcccg ccgtcttctt tgtgaactgg tatcgcagag atcgctcggg
     2761 ccgctggctc tggccgggtt ttggcgagaa cagccgggtg ctcaaatgga tgtgcgagcg
     2821 cgtcgaaggc aagattgacg ccgtgcgcac tccgatcgga tggatgccca ggaacggcga
     2881 cctcgacgtg aacggcctca cgatccggtc ggacgacttc gccgagctga tgcgcgtgtc
     2941 ggtcgagccc ttgagaaaag acctctcgga tgccgagcag tacttcgccc agttcggcga
     3001 caggctgccc gacaaactga gagtcgaact cgatgcaatg aggcaaaggc tggccgccgc
     3061 gggatgattc gatcgcgctt gtgtggggga cccatcaagg gactgacgcg atccgatcac
     3121 tcgtcgcgca cttcgtcgcc tgtgcgggcc tcgccgctca tgatgtcgcc ggccataaaa
     3181 ctgccgttgg gagcgcctgt cattcgcacc gtgcccaccg cctggttggt ccggaacacc
     3241 gtgagacgct gttcgggacg gggcagggga ttgaatccga aatcgaccac cacgaacatg
     3301 gcctgggtgt tgacccccgt cactttcccg atgctgcgct cgatgacggt cacgatcggg
     3361 tagcgtgtgg gagccggccc gggggcgggg cgggtggcgc ccgattggga agggccggcg
     3421 accgcgttgg tgacggcttc gatgggtggt tgggggcgat acgaaggcgc agtcgggacg
     3481 caccccgtca gcagcagaat cagacaccag ggcgttcggg ttgtggccgg caagttcatt
     3541 gtggggggct gcgcgagatg aattgacggc tttcgagttt ctggcaaatg gccgcccagc
     3601 ggcaatccca atcggcccaa ctggccgccc actcctcgcc ggcgtatcgt gtgcgtgccg
     3661 tcggcaggag cgccgccgtg tccgtgtaat gccgtgccgc ctcggtttcc tcgctccagg
     3721 caagcaacgt ctcgatcagg ttcaaacggt tctcgaccga gtccgggcag cgttggacgg
     3781 cccggagaag gtgggcgcgc gctttggact tgctcccaag gctcagcggc caaccggggg
     3841 catcgcgata gaggaggccc agacagcgat caggacccgc atgatcaaag tccggatcaa
     3901 gatcaaggac ctctttgaag tgtttctcca tctcagacac cagagagagg gccccgagga
     3961 ggcgggtccg ggccagttgc ccgaggttca tggccaggta gtaccgcgcg ggcgccagcc
     4021 tggggtgtcg acgcagcacg gagcgcgagg cggcgattcc ctcctgggcg atggcggcgc
     4081 gctgggtatc gttggtggcg aaatcggccc aatcgtagca ggcgcgggcg aagtgccagg
     4141 cggcagaagg atcggaggac tctcgatcac gacgttcgcc cgcggcgctg tagatacgtt
     4201 cggcccgcgc acggaacgcg gcggtgtcga ccgtgtttgt ttcggacgcg atgcaggcga
     4261 gagtcccagt cagagagaga aaggccagcc agttgatgga ccttttcata gggagggccc
     4321 atcccgcggt tgcgggagag cccccgattg gcgacggcaa ttgcctgacg ccctggagtg
     4381 gaggagttcg gccctccgag agtggttcag gtgcgaactg gctgctgg
//
LOCUS       DAOQRO010000291         4418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21066_length_4418_cov_4.913821, whole genome shotgun sequence.
ACCESSION   DAOQRO010000291 DAOQRO010000000
VERSION     DAOQRO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21066_length_4418_cov_4.913821"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="PKK20_10485"
     CDS             complement(<1..1976)
                     /locus_tag="PKK20_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNV00355.1"
                     /translation="MHSWMPFLLTLAACSAMAAGGDVDDLRLVPFPKEVRRLAGEFVL
                     GRALRLEAPGAQAEVLGRLLNEELRRAGIDPVQVRARGMPGPESSTGEMAGSHVKFLC
                     CVRRVEDSAERGAGEQGGETAGLVQEGEDRVGQRRASLGDRPESYELEVRGDEVVCAA
                     SDAAGLHYGLQTLCQLIRANRRGDALPCLAIRDWPSLRWRCFQDDMTRGPSSRIETLQ
                     FEASLGSFLKFNLMTFYMEYQYAFKKHPRIGPPDGSLTPDELSALVAYAKPLQIDILG
                     NQQSFGHFGHILKHPEYAALRETADVLTPMREETYRLLGDLYSEVCPLLPFPWFNVCC
                     DETWGLGTGPSKALASEIGVGGVYVRHIRRVHALLQEKHGKRMMMWGDIILKHPDQLE
                     QIPKDTLMLTWGYDARPSFEDQIVPFAKSGYEFFVCPGVNNWSRILPDFGVAITNIQH
                     FVRDGVKHGALGMLNTDWEDDGEAINAVKWHADAWAAECAWNGSTTSIESFNRRVGAV
                     LFGEAGDHFGRAVALLARTHRLRAMKGMMNARFWEDDFAPKAQPAAIESSATNLLAIV
                     RPAIEWLERCRAEARCNRQVLDAVLFGARRMELIGQRMLDGLEAARLYEAACSANAGA
                     EELVPVERLIRKNRDAHEALGRAFASLWLAESKP"
     gene            complement(2096..3580)
                     /locus_tag="PKK20_10490"
     CDS             complement(2096..3580)
                     /locus_tag="PKK20_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV00356.1"
                     /translation="MSGSADCPAAEPPTIFERLRRNFALRLNLWHALIFIACNGLLFL
                     LVYYVLVAAIERKESEVLEARLKEYALIYQSGGLSGLRRWAAQNKETGKQAQFFVRLV
                     DRWNQQFVVQVPDEWVSFRDVETGWEGYRRQVGVIRIPKDTERDFALVSMGFADGSLL
                     QVGRSANSRNTLLRPVQRAFLLALGATVLLGFTGGALLAHRASRPLRQIIATARSIIQ
                     TGQLDARVPLRESPDELNELARLFNTMLDKNQALIKAMRESLDNTAHDLRTPLTRMRG
                     VAELALQDPANAAKGREALADCVEESDRVLGMLKVLMDIAEAEAGMMSLQREHVEIGA
                     LLAQVIEVYAFVADEKRIAVHREFGEPRHVWIDANRLRQVFGNLLDNALKYTPQGGEV
                     RIRVDHDPRHVIVRVRDNGIGIPADEQNKIWGRLYRGDKSRSQRGLGLGLSLVKAVIE
                     AHGGRVSVSSESGRGSEFTVWLPELTDAKRASRRGPGGAVPLCA"
     gene            complement(3610..>4418)
                     /locus_tag="PKK20_10495"
     CDS             complement(3610..>4418)
                     /locus_tag="PKK20_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00357.1"
                     /translation="SDSLSEEERVQAFDDLINGADEKAVRAEAERWTKEAKEIVEPSA
                     EDINKAARISVALQQLMEREQAQGLAIGTCMGWLPKGFPCLGFARLRDVGIPAACEGD
                     MDSLLTMLVFGYAIDRAGFQGNATFDTSRNVVWTAHCTAPLKMEGVAGKQSPYLLRGH
                     SEVGGSGCVPEVLYRVGQTITRTKLVNLDTLLASTGKIVEVPSRSVRACRTQIVTEVR
                     DAAKMAANWSSVLETEDAMTLLHRVVFYDDQMDNARHLCRLMDMKLVEEG"
BASE COUNT          790 a         1462 c         1389 g          777 t
ORIGIN      
        1 taaggcttgc tctcggccag ccagagggac gcaaacgccc ggcccagggc ctcgtgtgca
       61 tcgcgattct tgcgaatgag ccgctcgacc ggtaccaact cctccgcgcc ggcattggcc
      121 gagcaggccg cctcgtacaa cctcgccgcc tcgagcccat cgagcatcct ctggccgatc
      181 aactccatcc gccgagctcc gaaaagaacg gcatcgagca cctgccggtt gcagcgcgct
      241 tccgcccggc accgttccag ccactcgatc gcggggcgca cgatcgcaag cagattcgtc
      301 gcgctcgatt cgatcgctgc gggttgggct ttgggggcaa agtcgtcctc ccagaagcgc
      361 gcgttcatca tccccttcat cgcgcgcagg cgatgggtcc gcgccaagag ggccaccgcc
      421 cggccgaaat gatcgcctgc ctcgccaaag agcaccgcgc cgacgcggcg attgaaactc
      481 tcgatcgagg tcgtggagcc gttccaggcg cactcggccg cccaagcgtc cgcgtgccac
      541 ttgaccgcat tgatcgcttc gccgtcgtct tcccagtcgg tattgagcat gcccagcgca
      601 ccgtgtttga cgccgtcccg gacgaaatgc tggatgttgg ttatggcgac gccgaaatcg
      661 ggcaggatgc ggctccagtt gttcacgccc gggcatacga agaactcgta gcccgacttg
      721 gcgaagggga cgatctgatc ttcgaagctg gggcgggcat cgtaacccca ggtcagcatc
      781 agcgtgtcct tgggtatctg ttcgagctga tccggatgct tgaggatgat gtcgccccac
      841 atcatcatgc gtttcccgtg cttctcctgc aacaaagcgt gaacccggcg aatgtggcga
      901 acgtacacac ccccgacccc gatctccgat gccaatgcct tcgacggacc ggtgccgagt
      961 ccccaggtct cgtcgcaaca gacgttgaac cacggaaacg ggaggagcgg gcagacctcc
     1021 gagtaaaggt cgcccaggag ccgatacgtc tcctcgcgca tcggcgtgag gacgtcggcg
     1081 gtctcgcgca acgccgcata ctccggatgc ttcaaaatgt ggccgaaatg tccgaacgac
     1141 tgttgattgc cgaggatgtc gatctgaagc ggtttggcat aagccaccag ggccgacaac
     1201 tcgtccggcg tcaacgagcc gtcgggcggg ccgatccggg ggtgcttctt gaacgcatac
     1261 tggtactcca tgtagaaggt catcaggttg aacttcagaa aggacccgag cgacgcctcg
     1321 aattgcagcg tctcgatcct cgaactgggg ccgcgcgtca tgtcgtcctg aaaacaccgc
     1381 cagcgcaaag acggccagtc ccggatcgcc aggcagggca aggcatcccc ccgacgattt
     1441 gcgcggatca gttggcacag cgtctggaga ccataatgca ggccggccgc atccgatgct
     1501 gcgcaaacca cttcatcgcc ccgcacctcg agctcgtagg attccgggcg gtcgcccagc
     1561 gaggcgcgtc tctgaccaac acggtcctca ccctcctgaa ccagccccgc cgtttccccg
     1621 ccctgctctc ccgcgcctcg ctcggcagaa tcctcgaccc ttctaacgca gcacaggaac
     1681 ttgacatggc taccagccat ttcaccggtc gaggattctg gtccgggcat cccccgggcc
     1741 cgcacttgga cggggtcgat cccggcccgc cgcagttcct cgttcaacag gcgccccagc
     1801 acctcagctt gtgccccggg ggcttcgaga cgaagggcgc gtcccagcac gaactcgcca
     1861 gccaatcggc ggacctcctt cgggaacggg accagcctca ggtcatcgac atcgccgcca
     1921 gccgccattg ccgagcaggc cgccagggtc agaagaaaag gcatccagga gtgcatgcgg
     1981 cacgataaaa caaccgcggg catggagctg gaaagacgaa aatagaagaa aaacgacttc
     2041 gaggtcccac tcggtttggg ggcttcggcc tgtctgtgct tccgcctatc cgcggctacg
     2101 cgcaaagcgg aacagcgccg ccgggcccgc gccgactggc tctctttgcg tccgtcagtt
     2161 cggggagcca gacggtgaac tcggagcccc ggcccgactc gctcgagacc gacacccgcc
     2221 cgccgtgggc ctcgatcacg gccttgacca ggctcaggcc caggcccagg ccgcgctggg
     2281 atcggctctt gtcgccccga taaagccgtc cccaaatctt gttctgctca tctgccggga
     2341 tgccgatgcc gttgtcgcgg acccggacga tgacatggcg cgggtcgtga tcgacacgga
     2401 ttcggacctc gccgccctga ggtgtgtatt tgagggcgtt atcgagcagg ttcccgaaca
     2461 cctggcgcag gcggttggca tcgatccaga cgtgccgggg ttcgccaaac tcccgatgga
     2521 cggcgattcg cttctcatcg gccacgaacg cgtaaacctc gatcacttgg gcaagcagcg
     2581 cgccgatctc gacgtgctcc cgctggagcg acatcatgcc cgcctcggcc tcggcgatgt
     2641 ccatcagcac cttgagcatc cccaggacgc ggtccgattc ctcgacgcaa tcggccaacg
     2701 cctcgcggcc tttggcggcg ttggccgggt cctggagagc cagttcggcg acgccgcgca
     2761 tccgggtcag cggggtgcgc aggtcgtgcg cggtgttgtc gagggattcg cgcatggcct
     2821 tgatgagcgc ctggttcttg tcgagcatgg tgttgaagag gcgcgccagt tcgttcaatt
     2881 cgtcgggaga ctcgcgcaac ggcacgcgag catcgagttg accggtctgg atgattgacc
     2941 gggcggtggc gatgatctgg cgcagcggtc ggcttgcgcg gtgggcgagc agggcgccgc
     3001 cggtgaaacc gagcagcacg gtcgcgccca gggcgagcag gaaggcgcgc tggactgggc
     3061 gcaggagcgt gttgcggctg ttggcgctgc gccccacctg cagcagcgat ccatcggcga
     3121 atcccatcga taccagcgcg aaatcgcgtt ccgtgtcctt cgggatgcgg atcacgccga
     3181 cttggcgtcg gtagccttcc cagccggtct cgacgtccct gaacgagacc cattcatcgg
     3241 ggacctggac gacgaactgc tggttccagc ggtcaacgag ccggacgaag aactgcgcct
     3301 gcttgccggt ctctttgttt tgggccgccc agcggcgcaa gccggacagt ccgccgctct
     3361 ggtagatgag ggcgtattcc ttgaggcggg cttcgagcac ctcgctttcc ttacgttcga
     3421 tcgcggcaac cagcacataa tatacgagca ggaacaggag tccattgcac gcgatgaaga
     3481 tcagggcgtg ccacaggttg aggcgcaacg cgaagttgcg cctcaggcgc tcgaagatcg
     3541 tgggcggctc ggccgccggg caatcagcag acccgctcat ctggcatcgg cggcccgccc
     3601 cgagtccgtt cagccttcct cgaccagctt catgtccatc agccggcaga ggtggcgcgc
     3661 gttgtccatc tggtcgtcat agaagaccac gcgatggagc aaggtcatgg cgtcttcggt
     3721 ttcgagcacg ctgctccagt tggcggccat tttggcggca tctcggacct cggtgacgat
     3781 ctgggtgcgg caggcccgaa ccgaccgcga cggcacctcg acgatcttgc ccgtcgacgc
     3841 aagcagggtg tcgagattga cgagcttggt gcgggtgatt gtctggccga ctcggtagag
     3901 gacctcggga acacagccgc tgccgccgac ctcggaatgc ccgcgcaaga ggtagggaga
     3961 ttgcttgccc gcgacacctt ccatctttag aggagcggtg cagtgggccg tccacaccac
     4021 gttgcgcgac gtgtcgaacg tagcgttgcc ctgaaaaccg gcgcgatcga tcgcataccc
     4081 aaagacaagc atggtcaaca aggagtccat gtcgccttca caggcggcgg ggatgccgac
     4141 gtcgcggagg cgggcgaagc ccaggcaggg gaagcctttg ggcagccagc ccatgcaggt
     4201 gccgatcgcg aggccctggg cctgttcgcg ttccatcaac tgctgcaaag ccacgctgat
     4261 gcgcgccgcc ttgttgatgt cctcggcgct gggctcgaca atctccttgg cctccttggt
     4321 ccatcgctcg gcctcggcgc gcaccgcctt ctcatccgcc ccgttgatca ggtcgtcgaa
     4381 cgcctggacg cgctcctctt cggaaagcga gtcggagc
//
LOCUS       DAOQRO010000292         4401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21214_length_4401_cov_5.582835, whole genome shotgun sequence.
ACCESSION   DAOQRO010000292 DAOQRO010000000
VERSION     DAOQRO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21214_length_4401_cov_5.582835"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PKK20_10500"
     CDS             <1..862
                     /locus_tag="PKK20_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00358.1"
                     /translation="ARWEKQEFMAPMGRALEGERALAALAYDPKRYSLRELVELTDRL
                     GSFNLFGASSASAPQSELEQLFQPVIELGGRCRQMMHLACWRFAWAKQDQLFHHRELQ
                     KQIETARRAAHDRRWKAKAAVPPDEDWQLYMRRGLFEKLNRYNRMRFWLALDTLTLSD
                     TALAKAAQMDVMREQIVAAIALKRHRLRHGRLPPTLEALVPEFLTSVPHDWFADAPMR
                     YRPVAGGAYELYSVNSDGVDDGGDSRNPRGDVRGWQSGRDLLWPRLASPEEVAAAESK
                     WVKAGGVKKR"
     gene            1005..1820
                     /gene="proC"
                     /locus_tag="PKK20_10505"
     CDS             1005..1820
                     /gene="proC"
                     /locus_tag="PKK20_10505"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNV00359.1"
                     /translation="MSATASIGFVGAGKMALALAQGWARAGLVAAKDIVSSDVREEGR
                     RDFVRDVGGRAVADNREVFGAVDVVVLAVKPDQAVAVLEAVRPAVAERHLIVSIAAGV
                     PLARLEGALFDGARVVRVMPNTPAMVGASASGFARGRWATAEDSSLTQRLFSALGVAV
                     EVPEKLLDAVTGLSGSGPAYVYQVIEALSDGGVAAGLPRSIATRLAAQTVFGAAKMAI
                     ETGLHPGVLKDMVTSPGGTTIEGLHELEKAGARGAFMNAVRAAADKARRLGEQ"
     gene            complement(2269..3129)
                     /locus_tag="PKK20_10510"
     CDS             complement(2269..3129)
                     /locus_tag="PKK20_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00360.1"
                     /translation="MPSKRVFDLSGESIAPRSRRAARIGHRRAFTLIELLVVIAIIAI
                     LAGMLLPALSGAKAKADRSLCSSNCRQWGVALQMYAVDNNDYFPDNRDGYDLSWVGTN
                     VVKFWDTYLIKSHKTKEEKNKFHVAFCPTDKWHRFADLWRNNDPSSENKPILTGYFFL
                     PHRNISGGWPYNSAGIQEWHARTKMGGPYRNAPVLIDRLQAIGSWSISANKGSVKWTT
                     VSDGKTVPTACHRVTGGKPSGGNFLFEDGHVQWRPFNLDNARATIDVGSFSGDWVLFY
                     KIPITTDTGM"
     gene            3378..4016
                     /gene="grpE"
                     /locus_tag="PKK20_10515"
     CDS             3378..4016
                     /gene="grpE"
                     /locus_tag="PKK20_10515"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNV00361.1"
                     /translation="MIESPKDEAAPDTDALPPCEPAMAAEPAVAASEVDGADTAASAA
                     GLALSAAEIEALQARAAKADENRNLYLRTAADFENFKKRAARERQESVRYANEALLGK
                     LISVLDNFEIALASAASLTGGSGESFRAGVALIQSQLRNVLVEAGLEEVDAAGQPFDP
                     NLHEAVAQRESSEVSDGHVLQQMRKGYKYRDRLLRPASVVVATALAPPASAD"
     gene            4039..>4401
                     /locus_tag="PKK20_10520"
     CDS             4039..>4401
                     /locus_tag="PKK20_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNV00362.1"
                     /translation="MAKRDYYEVLGVERAVTEEDLKKAYRKLALKYHPDRNPGDKVAE
                     ERFKELGEAYDVLSDPQKRAAYDQYGHAAFDPRQRAEAGGRWGGGGFHDPFEIFREVF
                     GGGGGIFEEFFGGGERRDP"
BASE COUNT          756 a         1252 c         1572 g          821 t
ORIGIN      
        1 ggcgcgctgg gaaaaacagg agttcatggc gccgatgggt cgggcgctcg aaggcgagcg
       61 cgcgctcgct gcgctggctt acgacccgaa acgctactcg ttgcgggagc tggtcgagtt
      121 gactgacagg ttggggagct tcaatttgtt tggagcctcg tcagcgagcg cgccgcagag
      181 cgaactcgag cagttgtttc agccagtgat cgaattgggg ggtcggtgca ggcagatgat
      241 gcacctggcc tgctggcggt tcgcctgggc caaacaggac cagttgtttc atcaccgcga
      301 gctgcagaag cagatcgaga ccgcccggcg cgcggcgcac gatcgacggt ggaaggccaa
      361 ggccgccgtt ccgcccgatg aggactggca gctatacatg aggagaggcc tgttcgagaa
      421 actgaaccgg tacaatcgaa tgaggttctg gctggctctg gacacgctca cgctctcgga
      481 caccgcgctg gccaaagccg cgcagatgga cgtgatgcgc gagcagattg tcgcagcgat
      541 cgccctcaag cgtcatcggc ttcggcatgg acggctaccg cccacactcg aggccctggt
      601 tccggagttc ctgacctccg ttccgcacga ttggttcgcc gatgcgccca tgcgctatcg
      661 accggttgcc ggcggggcgt acgagttgta ttcggtgaat tcggacgggg tagatgacgg
      721 cggagacagc cggaatcctc ggggcgatgt tcgcggatgg caatccgggc gcgatctcct
      781 ctggcctcgt ctggcctcgc ccgaggaggt ggcggcggcg gaatcgaagt gggtgaaggc
      841 ggggggagtg aagaaaaggt aggagtttcc tggtgcacgt ggagattatg attacgatta
      901 ggtcgtgtgt cgagtcatgg gccggcggtc tgaccccgag ttgggtggct gactattcgc
      961 ttttgccttt cgcgtgaacg gcttgggtgc taggctcagc ctcgatgtca gcaaccgctt
     1021 cgattgggtt cgttggcgca gggaaaatgg cgctggcttt ggcgcaaggc tgggcgcgcg
     1081 ccggcctggt ggcagcgaag gatatcgtct cgagcgacgt gcgagaggag gggcgccggg
     1141 attttgtccg tgacgtgggg gggcgcgcgg tggcggacaa tcgggaggtg ttcggagcgg
     1201 tcgatgttgt tgtgctggcg gtcaagcccg accaggcggt tgcggtgctc gaagcggttc
     1261 ggccggccgt ggccgagcgg cacttgatcg tatcgatcgc ggccggggtg ccgctggcgc
     1321 ggctcgaagg cgcgcttttc gacggggcgc gtgtggtgcg cgtcatgccc aatacgccgg
     1381 cgatggtcgg cgcctcggcg tcgggctttg ctcggggacg ctgggcgacg gccgaggact
     1441 cgagtctgac gcagcggttg ttttcggcgc tgggcgttgc cgtcgaggtg ccggagaagc
     1501 tgctcgatgc ggtcacgggt ctgagcggga gcgggccggc ctacgtgtac caagtgatcg
     1561 aggccttgag cgacggcggt gtggccgccg ggctgccgcg ttcgattgcc acgcggctgg
     1621 cggcgcagac cgtatttggc gcggccaaga tggccatcga gaccggactc catcccgggg
     1681 tgttgaagga catggtgacc agtccgggcg gcacgacaat cgaaggcctg cacgagctcg
     1741 agaaggcggg cgcgcggggc gcatttatga atgcggtgcg tgccgccgca gacaaggcga
     1801 gacggttggg ggagcagtga gggagcacag aagcgcgtcg cgcgatagcc attccacctc
     1861 caaccccgaa gcagacgcac atcgtccgga tggcggtcac gggggctgcg gtcgcactga
     1921 tcctggcgct gctggtcggt ctggtgtggg ggttggggcg gatactgtcg gtcctttcgc
     1981 gtgttctctg accgttggct gttaagaacg caaaggactc aatccccggg acacaccccc
     2041 ccgcaggccg acacaccgcc tctctccctc tccatctgtg ttccgccctg aacccgtgtg
     2101 gaacccctca ccgctcgagt gctcaggcag tccccacacg gattcagggc ggaacacaga
     2161 atggaatcga gacggtgatc ctgccggccg catccacgca gctttgtact gctcagtgtg
     2221 ccggatggcg tggcaggcgt ggaagcactg ctctcgccgg gatgtgtgct acattccggt
     2281 atcggtggtg atggggattt tgtaaaacag cacccaatcg ccgctgaatg agccgacatc
     2341 gatggtggca cgggcattgt cgaggttgaa gggacgccat tggacatggc cgtcctcgaa
     2401 caggaaatta ccgccgctgg gtttaccacc ggtgactcga tgacacgctg tgggcaccgt
     2461 cttgccatcc gatacggtcg tccacttgac gctccctttg ttggccgaga tgctccagga
     2521 cccgatcgcc tgcaggcgat cgatcagcac aggggcgttc cgataggggc cgcccatctt
     2581 tgtgcgggca tgccactcct gaatgccggc cgaattgtag ggccaccctc cggagatgtt
     2641 gcggtggggc aggaagaagt aacctgtcag aattggcttg ttctcggagc tggggtcgtt
     2701 gttgcgccag agatcggcaa accgatgcca cttgtcggtc ggacagaagg ctacatggaa
     2761 cttgttcttc tcctccttgg tcttgtggga cttgatcagg taggtgtccc agaacttgac
     2821 cacgttggtg ccgacccagc tcaggtcgta gccgtcacgg ttgtcgggga agtaatcgtt
     2881 gttgtcgaca gcgtacatct gcagcgccac gccccactgg cggcagttgc ttgaacagag
     2941 cgagcgatcg gccttggctt tcgccccgct cagagccggc aggagcatgc cggcgagaat
     3001 cgcgatgatg gcgatcacca ccagcagctc gatgagggtg aaggcgcgcc tgtgtccgat
     3061 gcgggccgcg cgcctcgatc gtggggcgat ggattcgccc gacagatcga acacccgttt
     3121 cgaaggcatc gggtcttgca tagcagttat ggagttcgag cattgttacc acgcgcccgc
     3181 ccagccgtca aacccggatg cgcaacccgc gcgcacggag atcataaacc gattcgtagc
     3241 cctcccgcgt tgcggggcgg ctagagaacc acggctgacg aagcgcgcag tgccttcttc
     3301 gaacagttgc ccagcgcccg cccttggtgt agtctctggg tctttaattt acggtcatga
     3361 gcaaagaaac tagtccaatg attgagagtc cgaaggatga agccgcgcct gacaccgatg
     3421 cgctgccgcc gtgcgaacca gcgatggctg cggagccggc ggttgccgcc agcgaggtgg
     3481 atggtgcgga caccgcggcc tcagcagccg gtttggcgct gagcgcggcg gagatcgagg
     3541 ccttgcaggc gcgggcggcc aaagccgacg agaatcggaa tctttacctg cgcacggccg
     3601 cggacttcga gaatttcaag aagcgggctg cgagggagcg gcaggaatcg gttcggtacg
     3661 cgaacgaggc tcttctaggc aagctgatct ctgtgctgga caacttcgag atcgcgctgg
     3721 cctccgcggc gagcctgacc ggtggttcgg gggagtcgtt tcgggcgggt gtggcgctga
     3781 tccagagcca gcttcggaac gtgctggtgg aggccggtct cgaggaggtg gacgccgccg
     3841 ggcagccgtt cgatccgaac ctgcacgagg cggttgcgca gcgcgagtca tccgaggttt
     3901 cggacgggca tgtgctgcag cagatgcgca agggctacaa gtatcgggac cgtctcctgc
     3961 gacccgcctc ggttgttgtg gccacggcgc tggccccgcc tgcatccgcc gactgagtgt
     4021 ttcttccacg gcaaggcgat ggccaaacgc gattactacg aggtgctcgg tgtggagcgg
     4081 gccgtcaccg aggaggacct gaagaaggcc taccgcaagc tcgccctcaa gtaccacccg
     4141 gaccggaatc ccggcgacaa ggtggcggaa gagcggttca aggagttggg cgaggcgtat
     4201 gacgttctga gcgaccccca gaagcgcgcg gcctacgacc agtacggcca cgccgccttc
     4261 gacccgcgtc agcgcgcgga ggcgggggga cgctgggggg gcggcggatt ccacgatccc
     4321 ttcgagattt ttcgcgaggt gtttggcggc ggcggcggga ttttcgagga gttctttggc
     4381 ggtggcgagc ggcgcgatcc c
//
LOCUS       DAOQRO010000293         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21249_length_4396_cov_5.295554, whole genome shotgun sequence.
ACCESSION   DAOQRO010000293 DAOQRO010000000
VERSION     DAOQRO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21249_length_4396_cov_5.295554"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..2145
                     /locus_tag="PKK20_10525"
     CDS             529..2145
                     /locus_tag="PKK20_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNV00363.1"
                     /translation="MNTPRDSESPHTSPLPDQGQRPPSGGALCGCHAHAETSAPSGFS
                     RRHFLQGLGAASAAVGALTLTVARVEGASERVAAAASFPRGATLRVKPVLVFDAPTRQ
                     EQTSWRSYGGIHTLDAAREEAGRIGKELTALKTQAEFPMHVHPVEVLDSQAKFRQMRV
                     DDTDACIVYAAGSCTEWPLEVPMIMFLRHRSGPFYLGLEIANWRFLRQSGDVPAVPGF
                     DADDLVVDSQAELLWRLRALYGVKNAKGTKMLTIGGLAAYSSLAQENGPRVAKEMWGY
                     EFVNVTDEEFTARLKAARENDAVVRQVEEQTAALLKLPNIKLETDRKFVFNSHMALHV
                     CRELLKETGASNFGFAQCMGHGQIAMLGTPACLVLSLANDEGYTAYCHTDLSHTMPGV
                     LLRWIASRPTFVCNSHHPHEGIFLVAHCQAPRRMNGQDYEPATIMTHYESDYGAACKT
                     EYKQDQVVTVVVPNLACNKWQGFRGKVIGSPSYPACRSQMEILVDGDWRRLQREMEGF
                     HTQVVYGDYLREVGYALKKVGRQIAWECFS"
     gene            2897..4300
                     /locus_tag="PKK20_10530"
     CDS             2897..4300
                     /locus_tag="PKK20_10530"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNV00364.1"
                     /translation="MIGAAGESSAPDRVVVKPEDNGAALINPDMGWTLMFYSNIPQNY
                     GSKLEPADTVDEWPGLSVVYLRLPWAFVEPEEGKFNWPVLDTPAQRWIAKGKRVAFRL
                     TCSENWTRFATPEWVKNAGAKGVFYEWGRGPKPDGPFWDPDFADPVFLAKLENFLRAL
                     AERYDGRPQVAFFDIGTYGMWGEGHTGGSSQIPQERANEIIRQHIDLHLKHFKRTRLA
                     IIDDVAGPQVEGAHQPLTDYALSKGITLRDDSICVQPPPNSWYHADMAGLFWPTLPVI
                     VEHEHYGPSKGRGAWGDGSLLIKAVEDYHASYLTIHWWPHEMLAETRETVDAINLRLG
                     YRLQLRSMSWPSRVAIGKPFEVESQWANAGVAPCYPGGFMALALKDAKGGIVAVLADG
                     TFNLRDLKPGPKGKAPVTSHRSRFTAGLIAPATASGTCDVFVSVGERDGTPVIALPLD
                     NDDGQRRYRLGSISLEP"
BASE COUNT          803 a         1364 c         1405 g          824 t
ORIGIN      
        1 gggcgggcag ttctcggaat tttcgacgca ccccgcttag gcgatgattt gggcccccca
       61 gcccttcgga atggggcggc accgggccat ctgcgtcgtt cctcggtcgg tcgagtatct
      121 caggggatac actccctccc tcgggccttg catctggccc ggtggcgctc ccgccaaaat
      181 cggaagtaat tattgcacag agcctaagga attgggtccg ctgccaagac ttgagagtat
      241 tgttgctgtt gcgagccggt gagctgcaac tgatccgcga cgctttcatg aagccgaaaa
      301 gatcggagtg gggacgcctg cctgccctgc ctacctgcac gctggcggca ggcagggccc
      361 gtcccccgcc atgccaagtg aagacggcgt ttgtcaacgg cggggttgcg cgcctctccg
      421 ttgccgggtt tgcacgcgcg cacgctgcat aacgaagctt ggcgcttgta acttgcggcc
      481 gggccaagta ttcacacggt cacggtcatc cagacatctg cataagccat gaatactcct
      541 cgcgattccg agtccccaca tacgtcaccg ctcccggacc aggggcagcg gccgccgtcg
      601 ggcggggccc tctgcggttg tcacgctcac gccgagacgt cagcgcccag cggtttctcg
      661 cgtcgccatt tcctgcaggg cctgggggcc gccagcgcgg ccgtcggggc tctgacactc
      721 accgtcgcca gggtcgaggg cgcgtccgaa cgcgtagccg cggccgcgtc gtttccccgc
      781 ggcgcgacgc tgcgagtcaa gccggtgttg gtgtttgatg cgccgacccg acaggagcag
      841 acctcgtggc gcagttacgg cggaatccac accctcgacg ccgcgcgcga agaagccggg
      901 cgcatcggga aggaactcac ggctctgaag acgcaggcgg agttccccat gcatgtgcat
      961 ccggttgagg tgctcgattc ccaggccaag tttcgccaga tgcgggtcga cgacacggac
     1021 gcttgcatag tctacgcagc gggttcctgc accgaatggc cgctcgaggt gccgatgatc
     1081 atgttcctgc gtcatcggtc ggggccgttc tacctcggtc tcgagatcgc gaactggcgg
     1141 ttcctgcgcc agagcggcga tgtcccggca gtgccgggat ttgacgccga cgacctggtg
     1201 gtggacagcc aggccgaatt gctctggcgg cttcgagcgc tgtacggggt caagaacgcc
     1261 aaaggcacca agatgctcac gatcggcgga ctggccgcct acagctccct ggctcaggag
     1321 aacggaccgc gcgtggccaa ggagatgtgg ggatacgagt tcgtcaacgt tacggacgag
     1381 gagttcactg cccgactcaa ggcggcgcga gagaacgacg cggtggttcg gcaggtcgag
     1441 gagcaaaccg ccgccctgtt gaagctgccc aacatcaagc tcgagacgga tcggaagttc
     1501 gtattcaact cgcacatggc cctgcacgtc tgccgggaac tgctcaagga gaccggggcg
     1561 agcaacttcg gcttcgctca gtgcatgggg cacggccaga tcgcgatgtt gggcacgccg
     1621 gcctgtttag tgttgagcct ggctaacgac gaaggctaca cggcctattg ccacactgac
     1681 ctgtcgcata cgatgccggg tgtgttgctg cgctggatcg cgagccgtcc gaccttcgtc
     1741 tgcaactcgc atcacccgca cgaggggatt ttcctggtag cccattgcca ggccccgcgc
     1801 cgcatgaacg gccaggacta cgagccggcg acgatcatga cgcactacga gtcggactac
     1861 ggcgcggcct gcaagaccga atacaagcag gaccaggtgg tgaccgtggt cgtgccgaac
     1921 ctggcgtgca acaaatggca ggggttccgc ggcaaggtga tcggctcgcc ctcctatccg
     1981 gcctgtcgtt cgcagatgga gatccttgtc gatggggatt ggagacgtct ccagcgggag
     2041 atggaaggat ttcacacgca agtggtctat ggcgactatt tgcgggaagt cggttacgcg
     2101 ctcaagaagg tcgggcgcca gatcgcgtgg gaatgtttca gttgagccga ctttccatga
     2161 atcgcctgcg caagccgttg ataattgaca aaaggggtaa cccgaacgtc caacgcgcaa
     2221 cgttcaacat ccaactttca acgaaccgct tcgttgaacg ttgagcgttg gaagttgggc
     2281 gttgaacgtt ccccggttca tgggtccaat gcgcattaaa ccgttgaatg acaacgcttt
     2341 ccataaatcg acactctcgg aggagccgat cccgcggcgg ccggagagga cctattaggt
     2401 ggtgatcatc gtctctggcc agggcgtctc ccgcgactgc gcatacgcgt caacctagtg
     2461 agatcccccg cgctgttcct ggaagcagcg ctgtcgaggt tgcctaagtc tctgtatcgg
     2521 cgttcgttgt ggcagtcgcc catgctctgg ttggcgtcga ggttgcagag cgtggggcgg
     2581 ggacgcctgc ctgccgcctg cctgccgcct gcctgccggc aggcagggca ggcagggcct
     2641 cctcgccccg aactccttca aatacattct tcggcggggg acgagccgtt cccccctcca
     2701 aggtacggtt catagagaac gttgacattc aacgctgtaa tgcgcatggg ggccagacca
     2761 gaagcgctca acgctcagcc gagaagacat gactcgccgc gccaaatgga gtagactcgc
     2821 cccgtatatg actcaattca cgaaagcacg tccttcgttt ctcttgtttg tcctcgtcgc
     2881 tgcggttttt gccgcgatga tcggggctgc gggcgagtcg tcggcgcccg atcgagttgt
     2941 cgtcaaacct gaggacaacg gcgcggcgct gatcaacccg gacatgggct ggaccctgat
     3001 gttctactcg aacatcccgc agaactatgg ctcgaagctc gagcccgcgg ataccgtgga
     3061 tgaatggcct gggctgtccg tggtctatct gcgcctcccg tgggcgtttg tcgaaccgga
     3121 ggagggcaag ttcaactggc cggtcctgga tacgcccgcg caacgctgga tcgccaaggg
     3181 caagcgcgtg gcatttcgcc tgacctgctc ggagaactgg acccggttcg ccacgcccga
     3241 gtgggtaaag aacgctggag ccaaaggcgt gttttacgaa tggggcaggg gaccgaaacc
     3301 ggacggccct ttctgggacc cagactttgc ggacccggtt ttcctcgcga agctcgagaa
     3361 tttcctgcgc gcgctggctg agcgttacga cgggcggccg caggtggcct tcttcgatat
     3421 cggcacctat ggcatgtggg gcgaaggtca caccggcggc agcagccaga ttccacagga
     3481 gcgcgccaac gagatcatac gccagcacat cgatttgcac ctgaagcatt tcaagcgcac
     3541 tcggctggcc atcatcgatg acgtggccgg cccgcaagtc gagggcgcgc atcagccgct
     3601 cacggattac gcgctctcga agggcatcac gctgcgcgac gacagcatat gcgtgcaacc
     3661 gccgccaaac tcgtggtacc acgcggacat ggccggtctt ttctggccca cgctcccggt
     3721 catcgtcgaa catgagcact atggcccgtc caaagggcgc ggcgcctggg gggatggctc
     3781 gctcttgatc aaggcggtcg aggactacca cgcgagctac cttactatcc attggtggcc
     3841 gcatgagatg ctcgccgaaa cacgggagac cgtggacgcc atcaacctcc gcctgggcta
     3901 ccggttgcaa ctgcgttcga tgtcatggcc cagccgcgtt gccatcggga agccgttcga
     3961 ggtggagagc cagtgggcca acgccggtgt ggccccgtgc tacccgggcg ggttcatggc
     4021 cctggcgctc aaggatgcca aaggcggcat cgtcgctgtg ctggccgacg ggaccttcaa
     4081 cctgcgcgac ctgaagcccg gtcccaaggg gaaggcccct gtcacatcgc accgcagccg
     4141 cttcacagcc ggtctgatcg ccccggcaac cgcgtcggga acctgcgacg tgtttgtgtc
     4201 cgtcggcgaa cgcgacggca cgccggtcat cgccctgccg ctcgacaacg acgacgggca
     4261 gcggcgctat cgactcggtt cgatttcact cgaaccgtag cccgcatggg gcaggttctg
     4321 gggttctcgg tttcgcggtt gtcacttacc acgtgaaggg gcgggcattc tgattttgcc
     4381 aaacaccgtc gcctcg
//
LOCUS       DAOQRO010000294         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21397_length_4374_cov_4.890947, whole genome shotgun sequence.
ACCESSION   DAOQRO010000294 DAOQRO010000000
VERSION     DAOQRO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21397_length_4374_cov_4.890947"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKK20_10535"
     CDS             <1..544
                     /locus_tag="PKK20_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV00365.1"
                     /translation="KHTTAWQDLQRAVRVVRSQAAAQGLDPDRIGIMGSSAGGHLTLM
                     GATSSKRRSYWPIDDLDKLSCSVQWAIAIYPAYALTDGADKPNDQGGNEDDARLAPEF
                     SFDLATCPILFIHGDADGWAAMNSVKCWEQLRRMGIQSDLHTLATREHCFQNKAAPGT
                     GSYTWMGRIWEFMNHKGFNK"
     gene            697..837
                     /locus_tag="PKK20_10540"
     CDS             697..837
                     /locus_tag="PKK20_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00366.1"
                     /translation="MKRFGIRLLPELYARLNPLPFGAAAQMEIDLAEDLRRAGYTVTG
                     GH"
     gene            917..1795
                     /locus_tag="PKK20_10545"
     CDS             917..1795
                     /locus_tag="PKK20_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNV00367.1"
                     /translation="MKSMTGYGRGEASRDGCKVTVEISAVNRRQAEVSLRLPPELEPL
                     ETRIREEINWRVARGRVNVRMTLHRSAKQWAASVRFNRPLVKAYACEFERLARELGID
                     EPVSLQAMLRIPGVVESTGADADPEALWPAVEAALGKALAGLGRMREREGAHLAKDLK
                     ARTAAMRRLSLDIARQAPGVVDRYRKQLLGRIRQAKLDLGADDDRLLKEVVMFADRAD
                     ISEELTRLQSHFRQFDDCMRSAEPVGRTLDFLAQEMNREVNTIGSKANDSGICQAVVR
                     MKAELEKVREQIQNLE"
     gene            1807..2427
                     /gene="gmk"
                     /locus_tag="PKK20_10550"
     CDS             1807..2427
                     /gene="gmk"
                     /locus_tag="PKK20_10550"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV00368.1"
                     /translation="MPIVLVISAPSGSGKTTVCSGLLAAAPGLARAITCTTRPPRPGE
                     RNGVDYHFLDPDTFGRRVAAGDFLEHATVYGHRYGTLKTELFDRLNARRDVLLNVDVQ
                     GAAAVRAEAARRPDLAAALVTVFLAPVSIDTLAERLRSRGQDSESVIGQRLAAARMEL
                     ERWREFDYLVVSGTMSEDLGCVQAIYTAEKLRCARMLRPPISEVQL"
     gene            complement(2418..3128)
                     /gene="radC"
                     /locus_tag="PKK20_10555"
     CDS             complement(2418..3128)
                     /gene="radC"
                     /locus_tag="PKK20_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNV00369.1"
                     /translation="MAPGSRIKDLPISERPRERLAQHGAESLSNADLLAILLRTGLRG
                     QSAVSVAEQLLSRFGRLDNLARASLAEIRSVRGIGLDKAIAVKAAFTLAQRMARDMAA
                     ESPLLDTPERVADLLREDNRLYEVECFQVVLLNTRRRLMRVEKLSQGTLDTLLVHPRE
                     VFRAAIAAGAAAIVVAHNHPSGDPTPSEQDIKVTRDLIRAGQLLKIEVVDHVILGRRT
                     AERSHDFVSLRELGYFYS"
     gene            complement(3226..>4374)
                     /gene="groL"
                     /locus_tag="PKK20_10560"
     CDS             complement(3226..>4374)
                     /gene="groL"
                     /locus_tag="PKK20_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNV00370.1"
                     /translation="AMDKVGKDGTITVEEAKSIETTLEVVEGMQFDKGYLSPYFVTNA
                     ETMECKLEDAYVLIYEKKISSLKDMLPILEKIAKVGKPLLIIAEEVEGEALATLVVNK
                     LRGTLNVCAVKAPGFGDRRKAMLEDIAILAGGRCITEDLGIKLENLEISDLGRAKSIV
                     VDKENTTIVEGNGKASEIQGRINQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVG
                     AATETEMKEKKARVEDALHATRAAVEEGIVAGGGVALVRCLGAIDAVKGANDDEKIGI
                     DIVKRAIVFPTYALADNAGVEGSVVVEEVKRRKGNEGYNVSTGQYEDMVKAGVVDPKK
                     VTRTALQNAASIAGLLLTTECLITEIPEKEKKTSGPGGHGMGGGDMDY"
BASE COUNT          736 a         1447 c         1408 g          783 t
ORIGIN      
        1 caaacacacg acggcgtggc aggacctgca gcgcgccgtc cgcgtcgtcc gcagccaggc
       61 cgcggcgcag gggctcgatc ccgaccgcat cggcatcatg ggctcctcgg ccggcgggca
      121 cctgacactc atgggcgcga ccagctcgaa acgccgttcc tattggccga tcgacgatct
      181 cgacaagctc tcttgcagcg tgcagtgggc gatcgcgatc tatcccgcgt acgcgcttac
      241 ggacggcgca gataaaccga acgaccaggg tggcaacgag gacgacgcgc ggctcgcccc
      301 cgagttctcg ttcgacctcg cgacgtgccc gatcctgttc atccacggcg acgcggacgg
      361 ctgggcggcg atgaattcgg tgaagtgctg ggaacagctc cgccgcatgg gcatccagtc
      421 cgatctgcac acgctggcca cacgcgagca ctgctttcag aacaaggccg cccccggaac
      481 cggcagctac acttggatgg ggcgcatctg ggagttcatg aaccacaagg ggttcaacaa
      541 gtagccgctt tggccgtgcg ccgtggaggg agcctcgagc ggttgttcca agactgcttg
      601 ctgcttttgg accagcagca gcgtcagagt cgcccctgac gcgtgcaatg gcttgcgcgg
      661 gaccagttgg gtcaagtgcg aaatgatgtc ccactggtga agcgcttcgg cattcgcctg
      721 ctgccggagc tttacgcgcg cctgaacccc ctgccgttcg gggcggcagc ccagatggag
      781 atcgacttgg ctgaggacct acgccgggcc gggtacacgg tcaccggagg gcattgatct
      841 tggtccgtgc tggcgcgctc ctgattcgca ttcctgattc gacatgcggc gcggccggcc
      901 tcatactcgg gttcgcatga aatcaatgac ggggtacggg cggggcgagg cgagtcggga
      961 tggatgcaag gtgacggtcg agatcagtgc cgtcaaccgg cgccaggccg aggtttccct
     1021 gcgtttgccg cccgaactcg agccgctcga aacgcggatt cgcgaggaga tcaactggcg
     1081 cgtggcgcgc gggcgcgtca atgttcgcat gacactccac cgcagcgcca agcaatgggc
     1141 ggcgtctgtc cgtttcaacc ggcctttggt caaggcgtac gcctgcgagt tcgagcgcct
     1201 ggcccgcgaa ctcgggatcg atgagcccgt cagcctgcag gccatgctgc gcatcccggg
     1261 cgtggtcgag tccacgggcg ccgacgcgga tcccgaagcc ctctggccgg ccgtcgaggc
     1321 cgctctgggc aaggcgctgg ccgggctggg gcggatgcgc gaacgcgagg gggcgcacct
     1381 ggcgaaggat ctcaaggccc ggacagccgc catgcgccgt ttgagtctcg acattgcgcg
     1441 acaggccccg ggggtggtcg accgctaccg caagcaactc ctgggacgca ttcggcaggc
     1501 gaagctcgac ctgggcgccg acgacgaccg cctcctcaag gaggttgtga tgttcgcgga
     1561 tcgggcggac atcagcgagg aactcacacg ccttcagagt catttccgac agttcgacga
     1621 ctgcatgcgg agtgccgagc ccgtcgggcg gacgctcgat ttcctggccc aggaaatgaa
     1681 ccgcgaggtg aacacgatcg gatcgaaggc caacgacagc ggcatctgcc aggcggtcgt
     1741 ccgcatgaag gccgaactcg agaaagtccg cgagcagatt cagaatctcg aatgacctcg
     1801 tcgtcgatgc cgatcgtgct cgtcatctcc gccccttcgg gaagcggcaa gacaaccgtc
     1861 tgcagcggcc tcctggctgc cgcgcccggc ctggcccggg ccatcacctg cacaacccgc
     1921 ccgccccgcc ccggcgaacg caacggcgtc gattaccact ttctcgaccc ggacaccttc
     1981 gggcgacggg ttgcggcggg cgacttcctc gaacatgcga ccgtgtatgg acaccggtac
     2041 ggcaccctca agaccgagtt gttcgaccgc ctgaacgcgc gccgggacgt gttgctcaat
     2101 gtcgatgtcc agggcgcggc cgccgtccgc gccgaggcgg cccgcaggcc ggatttggct
     2161 gcggcgctgg tcacagtgtt cctcgccccc gtctcgatcg acaccctggc cgaacgtctc
     2221 cgcagccgcg gccaggattc cgaatccgtg atcgggcagc gtctcgccgc ggcgcggatg
     2281 gaactcgaac gctggcgcga gttcgattac ctggtcgtga gcggcacgat gagcgaggat
     2341 ttgggctgcg tccaggccat ctacacggcc gagaaactgc gttgcgcacg gatgctccgg
     2401 ccgccgattt ccgaggttca actgtagaag taccccaatt cgcgcagcga cacgaaatcg
     2461 tggctgcgct cggcggtgcg ccggcccagg atcacatggt cgaccacctc gatcttgagc
     2521 aactgcccgg cgcggatcag atcgcgggtg actttgatat cctgctcgct gggtgtggga
     2581 tcgccgctgg gatggttgtg cgccaccacg atcgcggccg cgccggccgc aatcgccgcg
     2641 cgaaagacct cgcgcgggtg caccagcagc gtgtcgagag tgccttgaga gagcttctcg
     2701 acccgcatca gcctccggcg cgtgttcaag agcacaacct ggaaacactc gacttcgtac
     2761 agacggttgt cttctcgaag cagatcggcc acgcgctccg gggtgtcgag caacggcgat
     2821 tccgccgcca tgtcccgcgc catccgctgg gccaaggtga aagcggcctt caccgcgatc
     2881 gccttgtcca ggccgatccc gcgcactgac cgaatctcgg caagcgaagc ccgggcgaga
     2941 ttgtcgagcc gaccgaaccg agacagcaac tgctcggcca cggacacggc cgattggccg
     3001 cgcagacccg tccgcagcag aatagcgagc aggtccgcgt tgctgaggct ttcggccccg
     3061 tgctgggcca ggcgctcgcg cggacgttcg ctgatcggca aatccttgat gcgactcccc
     3121 ggcgccatcg cgcgaacagt cgaccaggat gagcggaaaa gcaagcgggg ccggcccagg
     3181 ccggccccgc atggtgaaac ctgcaagtcg aactgtcgaa ctggatcagt agtccatgtc
     3241 gccgccgccc atgccgtggc cgcccggacc cgacgtcttc ttctccttct ccggaatctc
     3301 ggtgatcagg cactcggtgg tgagcagcaa accggcgatg gacgccgcgt tctggagggc
     3361 ggtgcgggtg accttcttgg gatcgaccac gccggccttg accatgtctt cgtactggcc
     3421 ggtggagacg ttgtagccct cgttgccctt gcgacgcttg acttcctcga ccaccacgga
     3481 gccctcgacg ccagcgttgt cggccagggc gtaggtgggg aacacgatgg cccgtttgac
     3541 gatgtcgatg ccaatcttct cgtcgtcatt ggcgcccttg acggcatcga tcgcgccgag
     3601 gcagcggacc aaagccacgc cgccaccggc cacgatgcct tcttccaccg ccgcgcgggt
     3661 cgcgtgcaga gcgtcctcga cgcgcgcctt cttctccttc atttcggtct cggtggccgc
     3721 gccgacgttg atgaccgcga cgccaccggc gagcttggcc aggcgttcct ggagcttctc
     3781 gcggtcgtag tccgaggtcg tctcctcgat ctggcggcgg atctggttga tgcgtccctg
     3841 aatctcggaa gccttgccgt tgccctcgac aatggtggtg ttttccttgt cgacaacaat
     3901 cgacttggcg cgaccgaggt cgctgatctc gaggttttcg agcttgatgc ccaaatcctc
     3961 ggtgatgcat cgcccgccgg ccaggatcgc gatgtcttcg agcatggcct tgcggcggtc
     4021 gccaaacccg ggcgccttga cggcgcagac gttgagcgtg ccgcgcagct tgttcaccac
     4081 cagcgtcgca agcgcttccc cttcgacttc ctcggcgatg atcaggagcg gcttgcccac
     4141 tttggcgatc ttctcgagga tcggcagcat gtctttcagg ctgctgatct tcttctcata
     4201 gatcaggacg taggcgtctt cgagcttgca ctccatcgtc tcggcgttcg tgacgaaata
     4261 gggcgacaaa tagcccttgt cgaattgcat cccctcgacc acctcgagag tggtctcgat
     4321 cgacttggct tcctcgaccg tgatggtgcc gtctttgccc accttgtcca tcgc
//
LOCUS       DAOQRO010000295         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21425_length_4371_cov_4.291474, whole genome shotgun sequence.
ACCESSION   DAOQRO010000295 DAOQRO010000000
VERSION     DAOQRO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21425_length_4371_cov_4.291474"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKK20_10565"
     CDS             complement(<1..452)
                     /locus_tag="PKK20_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV00371.1"
                     /translation="MINLDSLNPPQREAVTTLRGPVLILAGAGTGKTRVITTRLAHMI
                     ERGVSPQHVLAVTFTNKAAREMLERVRALLPRPARPTDGASAPGPTICTFHALGVRIL
                     RENIERLGYKRHFVIYDESEQLATIKRILSRISVRGESVDPQAVLSML"
     gene            complement(461..1537)
                     /locus_tag="PKK20_10570"
     CDS             complement(461..1537)
                     /locus_tag="PKK20_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00372.1"
                     /translation="MSSPYTVLVVGMGKRGMHHATAFNANPKFKVTGICDIDAARLDA
                     AAAKLGHPEKSTNAAALARALRPDVFCFCTLPSLRLPMIRDGLEGGARLLAFEKPVAL
                     TSVELLDLRNLVRQAKAKAVVSHQHRYGQHYQKVKAIAASGALGRVHTVYGTATGWMT
                     HMLSHLIDYTCWFNDYEPGVWAMAQAAGRTKFADNHPSPDYIGGFVQFANGVRGIYEC
                     GAGAPDQPEVAKWWGKNRIGAQGTEGFAEVLTNGGWRAVTKSGGCQSGEGAMNYDLDM
                     PPYIQQMADWLDDDAKVHPCNLDHASLGAEIMLALQRSAAEGGQVALPLTVGTDEQAL
                     LKAKVPELPVLVSCEQNRKEFGLG"
     gene            complement(1750..2694)
                     /locus_tag="PKK20_10575"
     CDS             complement(1750..2694)
                     /locus_tag="PKK20_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00373.1"
                     /translation="MTRALLPRAWSDRPKPGYAMNNQSASASVAGIMGGVAAAFACLA
                     IVLALQNRSLQNQISDTRRLWSEAVASIADLRVQHAASTTDLSSHRQRLADLQQEIAE
                     LESRASSNVAVSSQPVPVRIFNGTRLVGWGWIPPSSTNRAGLAGEGVATVFLDSRNTS
                     VPPADNPTPGDPRPASPRIVTVTSSAFTYQHYPYFWPAIWLGPNPDRDHERGHDHPPQ
                     PSAPSAPEIQQRSTPTPAPAPAQRQVLRTVPQRLPTFSQPAARLAPFPQRPQVPAPIE
                     PFVAPSPSPSLRGSSAAIVRPAKSSAARQPIDRTTATH"
     gene            3025..3100
                     /locus_tag="PKK20_10580"
     tRNA            3025..3100
                     /locus_tag="PKK20_10580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3058..3060,aa:Thr,seq:ggt)
     gene            3160..4350
                     /gene="tuf"
                     /locus_tag="PKK20_10585"
     CDS             3160..4350
                     /gene="tuf"
                     /locus_tag="PKK20_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNV00374.1"
                     /translation="MAKEQFVRTKPHVNIGTIGHIDHGKSTLTAAIVEVQAAKGLATP
                     ISYADITKGGTVRDETKTVTIAISHVEYQSNLRHYAHVDCPGHADYIKNMITGAAQMD
                     GAILVVDAAEGPMPQTREHILLARQVGVPAIVVFLNKVDLVDDPELLELVDMELRDLL
                     TKYGFPGDKTPIVRGSSKKALAGDPEAMESIQKLLDAVDEFVPLPMREVDKPFLMSIE
                     DVFNIEGRGTVVTGRVERGELNRMQDVEIVGLRETRKTVATDIEMFRKLLDKASAGDN
                     VGVLLRGTKKEDVERGMVLAKPGTITPHTHFKAEVYVLSKEEGGRHTPFFTNYRPQFY
                     FRTTDVTGNVSLPEGVEMVMPGDNVSVTIKVIAPIAMERGQRFAIREGGRTIGAGRVT
                     EVIE"
BASE COUNT          796 a         1249 c         1528 g          798 t
ORIGIN      
        1 ctgagcatgc tcagcacggc ctgcggatcg accgactcgc ctcgcaccga gatccggctg
       61 aggatccgct tgatcgtggc caattgctcc gattcgtcgt agatgacaaa gtgccgcttg
      121 tagccgagac gctcgatgtt ctcgcggaga atgcggacgc caagcgcgtg aaatgtgcag
      181 atcgtcgggc ccggggccga tgcgccgtcg gttggccgcg ctggccgcgg cagcagcgct
      241 ctgacgcgtt cgagcatttc acgggcggct ttgttggtga atgtgacggc cagaacatgc
      301 tgcggtgaga cgcctcgctc gatcatgtgg gccaagcggg tggtgatgac gcgcgtcttg
      361 cccgtgcccg cgcccgccag gatgagcacc ggcccgcgaa gagtcgtcac cgcctcccgc
      421 tgcggtgggt tgagagaatc gaggttgatc atcgagaccg tcagcccagc ccaaactcct
      481 ttcggttctg ttcgcaggag acaaggacag gcagttcggg caccttcgct ttcagcagag
      541 cctgctcgtc ggtcccgacg gtgagcggga gcgcgacctg gccgccctcg gccgcgctgc
      601 gctggagggc gagcatgatc tcggcgccca ggctggcgtg gtccagattg cacggatgca
      661 ctttggcgtc gtcgtcgagc caatcggcca tctgctggat gtaaggcggc atatccaggt
      721 catagttcat cgccccttcg ccgctctggc agcctccgga ctttgtgacg gcgcgccacc
      781 cgccattggt gagcacctcc gcgaagcctt cagtgccttg ggcgccgatg cggttcttgc
      841 cccaccactt ggcgacctcg ggttggtctg gcgcgcctgc gccgcactcg tagatgccgc
      901 gcaccccgtt ggcgaattgc acgaaaccgc cgatgtaatc gggcgaggga tgattgtcgg
      961 cgaacttggt gcgaccggct gcctgggcca tggcccagac gcccggctcg tagtcgttaa
     1021 accagcaggt gtaatcgatc aggtgcgaaa gcatgtgcgt catccagccc gtcgccgtgc
     1081 catagaccgt gtggactcgt ccgagggcgc cgctcgcggc gatggctttg actttctggt
     1141 agtgctggcc atagcggtgc tggtgactga cgacggcctt ggctttggcc tggcggacca
     1201 ggttgcgaag atcgagcagt tcgacgctgg tcagggccac gggcttctcg aatgccagaa
     1261 gccgcgcccc gccttcgagt ccatcccgga tcatcggcag acgcagactc ggcagggtgc
     1321 agaagcagaa gacgtccggg cgcagagcgc gggccagggc ggcggcattg gtgctcttct
     1381 ctggatggcc aagcttggcg gctgcggcgt cgaggcgggc ggcgtcgata tcgcagatgc
     1441 cggtcacctt gaacttcgga ttggcgttga aggctgtggc gtggtgcatt ccgcgcttgc
     1501 ccatgcccac gacgagcacg gtgtagggac tactcacggt tttcagaatc gcgtattcaa
     1561 tcttccatga caaaacactg gcgcgatact gaagatgccc gggcctgatg accagtgcga
     1621 tttcggaggg ctgaactgcg agaggccctg attgatggcg gttattgcat cgcgtcaggc
     1681 actccccccc gcgggcccgg cccatcgagt ccggcctctc caagtccgga ttctccccca
     1741 aataaggatt caatgcgtcg ccgtcgtccg gtcgatcggt tggcgcgcgg cactgctttt
     1801 tgcggggcgg acgatcgccg cagatgagcc gcgcaggctg ggcgaagggg acggggcgac
     1861 gaacggctcg atcggagcgg ggacctgagg gcgctgagga aaaggagcga ggcgcgccgc
     1921 gggctgggag aacgtaggca gccgctgcgg gactgtcctg agcacctggc gctgagcagg
     1981 cgctggggcc ggcgtcggcg tcgagcgctg ttggatttcc ggcgcggatg gcgcggatgg
     2041 ttggggcggg tgatcgtgac ctctctcgtg gtcgcgatcc ggattggggc ctagccagat
     2101 ggcgggccag aagtatgggt agtgttggta ggtgaaagcc gagctggtga ccgtgacgat
     2161 cctcggagag gcgggccggg gatcgccggg ggtcgggtta tcggcgggag gcaccgacgt
     2221 attgcgactg tccaggaaga ccgtggcgac accttcgccg gccaggcccg cccggtttgt
     2281 ggacgatggc ggaatccagc cccagcccac gagacgggtc ccgttgaaga tccggacggg
     2341 gacgggctgg ctgctgacgg cgacattgct tgacgccctc gactcgagct cggcgatttc
     2401 ctgctgcaag tccgccagcc gctggcggtg cgaggagagg tccgtcgtgg atgcggcgtg
     2461 ctggacgcgc aaatccgcga tggaagcaac ggcttcggac cagaggcggc gggtgtctga
     2521 aatctggttc tggagcgagc gattctgcag cgccaggacg attgccagac aggcaaatgc
     2581 cgcggcgaca ccgcccataa ttcctgcgac cgacgctgat gccgactggt tgttcatcgc
     2641 gtaacccggt tttggccgat ccgaccaggc ccgcgggagc aacgcacgcg tcacgcgcgg
     2701 gctcgatcca cgggacaggg ccgtgctgtg cgacagccgc cgccggagtc gggtcggttg
     2761 ttccgacagg acagtggctt accggaaccg gtttggcaag cagcggtcct cgaccttgaa
     2821 gtgagcttcg cgctgggtcg caaaaagttc atcgaaattc gttgacaccg gggggtgcgg
     2881 tctcctaaat tcacgcgctc aaaactgatt tgtgaagagc tgacaaccga ttgtccccgc
     2941 ccagatggag caagccagag ggtgaggggc ggaggtggtt tgccgttttg agatggcgac
     3001 gaaacgggaa ttggtgtggg tttcgcccat gtagctcagc cggcagagcg cgtccttggt
     3061 aaggacgagg tcaccagttc aaacctggtc atgggctcca acttttaagt gattgattga
     3121 gagtggcaac cgaaatcgaa gagaacagca ggaatcgcaa tggctaaaga gcaatttgtg
     3181 cggacgaaac cgcacgttaa cattggcacc atcggtcaca ttgaccatgg caaatcgacc
     3241 ctgaccgccg cgattgtcga ggtccaggcg gcaaaaggct tggccacgcc gatctcctac
     3301 gcggacatta cgaagggtgg cactgttcgg gacgaaacca agacagtgac catcgcgatc
     3361 tcgcacgtcg agtatcagag caacctgcgc cattacgccc atgtcgattg ccccggccac
     3421 gcggactaca tcaagaacat gatcacgggt gcggcgcaga tggacggcgc catcctggtg
     3481 gtcgacgcgg ccgagggtcc gatgccccag acgcgcgagc acatcctttt ggcccgccag
     3541 gtgggcgtgc cggcgatcgt ggtcttcttg aacaaggtgg atctggtgga cgatccggag
     3601 ctgctcgagc tggtcgacat ggagttgcgg gatctgctga ccaaatacgg ttttcccggc
     3661 gacaagacac cgattgtgag gggaagctcg aagaaggcgc tggctggcga tcccgaggcg
     3721 atggagtcga ttcagaagct tctcgatgcg gtggatgagt ttgtgccgct cccgatgcgg
     3781 gaggttgaca agccgttcct gatgagcatc gaggacgtgt tcaacatcga agggcggggc
     3841 acggttgtga ctgggcgagt cgaacgcggc gaactcaacc gcatgcagga cgtcgagatc
     3901 gtcggtttga gggagacgcg caagaccgtg gcgacggaca tcgagatgtt ccgcaagctg
     3961 ctggacaagg ccagtgcggg cgacaacgtc ggcgtgctgt tgcgggggac gaaaaaggaa
     4021 gacgtcgagc gcggcatggt gttggccaag ccgggcacca tcacaccgca cacccatttc
     4081 aaggccgagg tgtacgtgct gtcgaaggaa gagggcgggc ggcacacgcc gttctttacg
     4141 aattatcggc ctcagttcta tttccggacg acggacgtga ccgggaatgt ttcgctgccg
     4201 gagggtgtcg agatggtcat gcctggcgac aacgtctcgg tgacgatcaa ggtgatcgcg
     4261 ccgattgcga tggagcgcgg gcagcggttc gcgattcgcg agggcggccg caccatcggg
     4321 gccgggcgcg tcaccgaggt catcgagtaa gcgagtaagt cgtgcgcccg g
//
LOCUS       DAOQRO010000296         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21570_length_4354_cov_4.234706, whole genome shotgun sequence.
ACCESSION   DAOQRO010000296 DAOQRO010000000
VERSION     DAOQRO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21570_length_4354_cov_4.234706"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..922)
                     /locus_tag="PKK20_10590"
     CDS             complement(404..922)
                     /locus_tag="PKK20_10590"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNV00375.1"
                     /translation="MLVRLWLTVERCFVLNLIRLFRIRGQSERVARGFALGLVVNFFP
                     TFGFGVVVSIALVRFLGGNLVAGVIGATSLSFVWPFLFYLNIRTGSLFVRPPLIIDEF
                     GDVNEKTMDALMWGQTFTLGSIVNSALAGLTAYLILRLLYRQIRPGALAYFRRHAREH
                     QRRFRRPARVQA"
     gene            complement(956..1669)
                     /locus_tag="PKK20_10595"
     CDS             complement(956..1669)
                     /locus_tag="PKK20_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNV00376.1"
                     /translation="MLYLIRELRQAYRLMRASHGHLHWWPASSPLEVCLGAILTQNTS
                     WMNVERAIANLKNAASIDPRHLLRLDHSKLAALLKPAGYFNVKTRRLRSFLSVLVNEF
                     DGELDRLFAGPTHQVRERLLGIHGIGPETADSMLLYAGGHLSFVIDAYTKRIFLRHAW
                     CPAEATYSHLQAVCVRSLSHTPRSARLDYWQDYHAQLVMVGKEYCRKRQPRCERCPLR
                     PLLPRSPALPRDCGGGVRS"
     gene            complement(1687..2928)
                     /gene="lepB"
                     /locus_tag="PKK20_10600"
     CDS             complement(1687..2928)
                     /gene="lepB"
                     /locus_tag="PKK20_10600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV00377.1"
                     /translation="MKLARGIWHWLTSRTVRQAVDQHRIVRRLHAYQHDRLPPENAAE
                     VQRALDDLRHALATAESLTAIHACSERLKSVATLRLDGLSRRLHRESLELVVMACVVV
                     LAARTFFLQPMTIPSGSMQPTLYGITVENLQSSPDVAVPTGLSRLIEWLVHGRRYFHF
                     VAKTDGQLTWIGPVEPAFPWLWFVPICHKQDFVVGGTRYRIWSPPPEVPNPFPSPQDR
                     AIFAHARLDPNRHYRTGEDLMKLVCTAGDRVLVDRLTYNFRRPRRGEIIVFRTTGITN
                     VQEGTYFMKRLVATGGERVRIGNDRHLVINGVRLDTDTPGFDQIYSFSGPPRDSRYSG
                     HMNDATARRYRIRPGTVAPRFPSERTEFVVRRGHCLVMGDNTLTSLDGRRWGDFRAEL
                     VIGRFWMVYWPISVRTGWSAH"
     gene            complement(2925..4094)
                     /gene="lepB"
                     /locus_tag="PKK20_10605"
     CDS             complement(2925..4094)
                     /gene="lepB"
                     /locus_tag="PKK20_10605"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV00378.1"
                     /translation="MHAILRWFLSRPFRDATELRKQVRMVLNSQRDLLPPRAIAEIET
                     GLAAFGDELRAASDKQAIRSASSRLEECAARWLQPYPNASARENIKEFLVSGVLILTV
                     FCFFLQPMKIPSGSAQPTLYGNVITPLPEGNPEAVIPPRAVRILHWFKGIDYHHWIAK
                     DSGPLRIEPVSRMLGFVRFQRFIVGKDSYTFYWPPDNLDRFCGAELGRHFEKGQTVLR
                     LKTSSGDRLFVDRFTYNFRRPKRGETIVFTSTGIPGLIQHTHYIKRLIAMGGERVRIG
                     DDRHAVVNGQRLDRHMRHFERVYAFSTPPRESVFSGHVNDKVAMEVLGRSIAPLFPDE
                     HREVIVRPNHYLAFGDNTLNSFDGRAWGDFPRDKVVGKGLFVFWPFTERFGLIYW"
     gene            complement(4100..>4354)
                     /locus_tag="PKK20_10610"
     CDS             complement(4100..>4354)
                     /locus_tag="PKK20_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979450.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNV00379.1"
                     /translation="LAAKLKEVIPRQQFDVAIQAAIGGKVVARETVSAFRKDVTAKCY
                     GGDISRKRKLLEKQKEGKKRMKSIGSVNIPQQAFIEVLKA"
BASE COUNT          849 a         1230 c         1441 g          834 t
ORIGIN      
        1 gaggctgcgg ccgccgcagt tgtcgcatcg agcccgttgg tgccggctgc ctgcacagcc
       61 agcgccacga caagcccggc gattccgatt gccagagggg cagcggcgct cagggcgtga
      121 agggagtgcc tcgatgattc agttcggtgt gcgttcatga cttcgttttc ctgttcgatc
      181 cagtcggcgt gccgcgtcct cgcgggagat ttcgtgccgg cgaatgccat cgcttgtgtt
      241 tacaggcgcg tctgtcgcca taatgtgcgt cgggtccgtt ggcctcatcc gtgcttgggg
      301 tctgtgcaaa aactaatcgg aagtaatgtt tgcacagacc cttaatcctc gatcgtggcg
      361 tcctcgctcg gatccatacc gtgctcggtt tcgatccgcc ggctcaagcc tgcacccggg
      421 cagggcggcg gaagcggcgc tgatgctccc gggcgtgacg acgaaaatac gccagcgcgc
      481 cggggcgaat ctggcgatag agcaaccgca ggatcaggta ggcggtgagc ccggccagcg
      541 cactgttgac aatcgaaccg agggtgaagg tctgtcccca catcaacgcg tccattgtct
      601 tttcgttcac atcgccgaat tcgtcaatga tcaagggagg gcgaacgaac aaactccccg
      661 tgcgaatgtt cagatagaaa agaaacggcc aaacgaagga cagagaagtc gctccgatga
      721 ctccggccac cagattgcca ccgaggaaac gaaccagggc gatcgagacg accacgccga
      781 acccgaacgt cgggaagaag ttgacgacga gccccaatgc gaatccgcga gccacccgtt
      841 cgctctgccc gcgaatgcgg aagaggcgga tcaggttcaa cacaaagcag cgctcaacgg
      901 tcagccagag ccggactagc atcggaccga atagtgtttt gtctccgtgc gccgttcaag
      961 agcgaacgcc tccgccgcaa tctcggggaa gggcagggga ccggggcagg agggggcgga
     1021 gcgggcagcg ctcacagcgc ggctggcgct tgcggcagta ttccttgccc accatgacaa
     1081 gctgagcatg gtaatcctgc caataatcga gtcgggccga tcgcggcgtg tggctgaggg
     1141 agcgcacgca gacggcctgg agatgcgaat aggtcgcctc cgcgggacac catgcatgcc
     1201 ggaggaagat tcgcttggtg taggcatcga tgacgaaact caaatggcca ccggcgtata
     1261 ggagcatgct gtcggcggtc tcggggccga tgccatgaat gccgagcaaa cgctcgcgga
     1321 cctgatgcgt agggccggcg aacaggcgat cgagctcccc gtcgaattcg ttgaccagga
     1381 ccgaaaggaa tgagcgcagg cgccgtgttt tgacgttgaa gtagcccgcg ggtttcagga
     1441 gtgcggcgag tttggagtgg tcgaggcgca ggagatgacg agggtcgatc gaggcggcgt
     1501 tcttgaggtt ggcgattgcg cgctcgacgt tcatccaact ggtgttttgg gtcaggatag
     1561 cgccgaggca gacttcgagc ggggaggatg cgggccacca atggaggtgc ccgtgcgagg
     1621 cgcgcatcag acgataggct tgacgcagtt cgcgaattaa atataacata tattgtgcga
     1681 attaaactaa tgcgctgacc agcctgttcg cacgcttatt ggccagtaca ccatccaaaa
     1741 tcgaccaatc accagttccg ccctgaaatc accccacctg cgcccatcca aactcgtcaa
     1801 tgtgttgtcg cccatcacca gacaatgccc ccgacgcacc acgaactccg ttcgctcgct
     1861 cgggaacctc ggcgcaaccg tccccggccg gatgcggtac ctccgtgccg tggcatcgtt
     1921 catgtggccc gagtatcggc tgtcgcgcgg tggacccgag aaactgtaga tctggtcgaa
     1981 acccggcgtg tcggtatcca ggcggacgcc attgattacg agatggcgat cgttgccgat
     2041 gcgcacccgt tcaccgcctg tcgctaccaa tcgtttcatg aaatacgtcc cttcctgcac
     2101 gttcgtgatc cctgtcgtcc tgaaaacgat gatctcgcct cggcgcggcc tgcggaagtt
     2161 gtaggtcaag cgatcgacca ggacgcggtc gcctgcggtg cagaccagtt tcatcaggtc
     2221 ttccccagtc cggtagtggc gattggggtc caggcgggcg tgggcaaaga tggcgcggtc
     2281 ttgcggagac gggaacggat tggggacctc gggtggaggc gaccatatcc gatagcgtgt
     2341 gccgcccacg acaaaatcct gtttgtgaca gattggcaca aaccacaacc atgggaatgc
     2401 cggctcgacg ggtccgatcc aggtcagttg accgtcggtt ttggcgacga aatggaaata
     2461 tcgtctgcca tggaccagcc attcgatgag acggctgagg cctgttggca cggcgacatc
     2521 gggcgaggat tggaggtttt cgacggtgat gccgtagagc gttggctgca tcgaccccga
     2581 ggggattgtc atgggctgga ggaagaacgt cctggctgcc aggacaacga cgcaggccat
     2641 gacgacgagt tcgagagact cgcgatgcaa tcggcgcgac aggccgtcga gccggagtgt
     2701 ggctacggac ttgagccgtt cggagcaggc atggattgcg gtcagggatt ccgcggttgc
     2761 gagggcatgc ctgaggtcat cgagggctcg ctgcacctcg gcggcgtttt ctggggggag
     2821 tcgatcgtgc tggtaggcgt ggagacggcg gacgatccga tgctgatcga cggcctggcg
     2881 gacggtgcgc gaggtcagcc agtgccagat gccccgagcg agcttcacca gtagatcaga
     2941 ccgaaccgtt cagtgaatgg ccagaagacg aagagcccct tgcccacgac cttgtcgcgc
     3001 gggaagtcgc cccaagcccg gccgtcgaag ctgttgagcg tattgtctcc aaaggccagg
     3061 taatggttgg gtcgcacgat cacctcccgg tgctcgtcgg gaaacaacgg tgcgatgctg
     3121 cggcccagga cttccatcgc caccttgtcg ttcacgtgac cggagaacac gctctcgcga
     3181 ggcggcgtgc tgaacgcgta aacgcgctcg aaatggcgca tatggcggtc gagtctctgt
     3241 ccgttaacca ccgcatgccg gtcgtcgccg atgcggactc gttcgcctcc catcgcgatg
     3301 aggcgtttga tgtagtgggt gtgctggatg agccccggaa tgccggtcga ggtgaacacg
     3361 atcgtctccc cgcgtttggg tctgcgaaaa ttgtaggtga accggtcgac gaacagccgg
     3421 tcgccgctgg aggtcttgag gcgcaggacg gtttgtcctt tctcgaagtg ccgacccagc
     3481 tcggcgccgc agaatcggtc gaggttgtcc ggcggccagt agaacgtgta ggaatccttg
     3541 cccacgatga agcgctggaa cctgacgaat ccgagcatgc gcgatacggg ctcgattcgg
     3601 agagggccgc tgtctttggc gatccaatga tggtaatcga tccccttgaa ccagtgcagg
     3661 atgcggaccg cgcgtggcgg gatgaccgct tctggattgc cttcgggcag cggggtgatc
     3721 acgttgccgt acaaggtcgg ctgggcagat ccggacggaa tcttcatggg ctgaaggaag
     3781 aagcagaaga cggtcaggat cagcacgccg ctcaccagaa actccttgat gttctctcga
     3841 gcgctggcgt tgggataggg ctggagccat cgcgcggcgc attcctcgag ccggctcgag
     3901 gcggaccgga tggcttgttt gtctgaggcg gctctcagtt cgtcgccgaa agccgccagc
     3961 ccggtttcga tctcggcgat ggccctgggc ggcaggagat cgcgctgaga attcaggacc
     4021 atgcggacct gcttgcgcaa ttcggtggca tcccggaatg ggcgggatag gaaccaacgg
     4081 aggatggcgt gcatgggcgt caggctttga ggacctcgat gaaggcctgc tgcgggatat
     4141 tgaccgagcc gatcgatttc atccgtttct ttccttcctt ctgtttttcg aggagcttgc
     4201 gtttgcggga gatatcgcct ccgtagcact tggcggtcac gtctttgcgg aaggcgctga
     4261 ccgtttcgcg ggccacgacc ttgccgccga tggcggcctg gatggcgacg tcgaactgtt
     4321 gccgcgggat aacctccttg agcttggctg caag
//
LOCUS       DAOQRO010000297         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21608_length_4348_cov_5.103890, whole genome shotgun sequence.
ACCESSION   DAOQRO010000297 DAOQRO010000000
VERSION     DAOQRO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21608_length_4348_cov_5.103890"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /gene="nikR"
                     /locus_tag="PKK20_10615"
     CDS             <1..336
                     /gene="nikR"
                     /locus_tag="PKK20_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNV00380.1"
                     /translation="NRSLAIGDMIRAHLVEHRQRLGDEEIAGTITLLYDHHQRHVQTA
                     LTDIQHDHHTVIIATLHVHLDHHNCMEVLAVRGKAAPIKRIADELIAAKGVKHGKLTV
                     TSTGKDLPG"
     gene            complement(333..1874)
                     /locus_tag="PKK20_10620"
     CDS             complement(333..1874)
                     /locus_tag="PKK20_10620"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNV00381.1"
                     /translation="MAAFSTQCLGAPPNVVIILADDLGFSDLGCYGSEIQTPHLDTLA
                     KQGLRFTQFYNTARCWPTRGALLTGYYAQQIRRDALPGLGGGAGGVRPHWARLLPDFL
                     KPAGYRSYHSGKWHVDGGVLAGGFDRSLDMRNQGNYFTAAGNRIDDAPLKSPANEQGY
                     YATIATADHAINCLKDHATSHAGKPFFSYIAFIAPHFPLHALPEDIAKYRDRYLAGWE
                     AMRADRFARQREMGLVRTTLSPLEPAVGPPYHFPEALEKLGAGEVNRPLPWSELTGKQ
                     RRFQATKMAIHAAMVDRMDREIGRIIGQLKAMGAYDNTLILFASDNGASAEIMVRDGG
                     HDPQAAPGCAATYLCLGPGFSSACNTPYRRHKTWVHEGGISTPLIAHWPKGITARNAL
                     RHTPAHVIDIVPTVLSVAGVEKPRAWAGQPIPEAPGMSLAPAFVEDVRIPRASLWWLH
                     EGNRAVRAGDWKLVASAGERWELYDLTTDRAEQHDLAAQRPDKVRELEEIWQRQTDSF
                     SELAR"
     gene            complement(2542..3630)
                     /locus_tag="PKK20_10625"
     CDS             complement(2542..3630)
                     /locus_tag="PKK20_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNV00382.1"
                     /translation="MAHLKLHIPGPVEVSEKTFRAFCSPMIGHRSQGFKDLYASIQPR
                     LQELLYTRQPVFLSTSSAWGVMEGAVRNLVHKKVLNCMCGAFSDKWNDVSKRCGKEAE
                     ALQVPWGSPIRTEDVDRKLATGAFDALTFIHNETSTGVMSPLAEVAALKAKYPDVMFI
                     IDSVSSMSAVKIEFDALGIDVLLAGSQKAFALPPGLTVFTCSAGALAKAAAAKDRGYY
                     FDLVEFQKNAAQSMTPSTPSIGHVHALASKLEDIFAEGLDARFARHAKLAGMTRDWAA
                     RHGFNLFPDKGFESMTLTCVNNGAKPGSRTVDVPKLQKLVKDQGFLIDGGYGKIKGTT
                     FRISNMGDETESSMQALYTALDQAMQQL"
     gene            3983..4216
                     /locus_tag="PKK20_10630"
     CDS             3983..4216
                     /locus_tag="PKK20_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00383.1"
                     /translation="MLVAATPSSLASIRAPYTPDANTKLLLHLDDATESGIAANAVAG
                     APSFIATANPSAATSRQPIPGILGAPGASGGVG"
BASE COUNT          787 a         1352 c         1432 g          777 t
ORIGIN      
        1 aaccgatcgt tggccatcgg ggacatgatt cgcgcgcacc tggtcgagca tcgacagcga
       61 ctcggcgacg aggaaatcgc cggcaccatc accctcctct acgatcatca ccagcgacac
      121 gtccagaccg cgctcaccga cattcagcac gaccatcaca cggtcatcat cgccaccctg
      181 cacgtccacc tggaccacca caactgcatg gaagtgctcg ccgtccgagg caaggcggcc
      241 ccgatcaagc ggattgccga cgaactgatc gccgccaagg gcgtgaagca cggcaagctg
      301 accgtcacca gcaccggcaa agacctgccg ggttagcggg ccagttcgct gaagctgtcg
      361 gtctggcgct gccaaatctc ttcgagttcc cgcaccttgt ccggcctctg cgcagccagg
      421 tcgtgctgct ccgcgcggtc ggtcgtcaga tcgtagagtt cccaacgctc gcccgcggac
      481 gcaaccagct tccagtcgcc cgcacgcacg gcccggttgc cctcgtgcag ccaccaaaga
      541 ctcgcgcgcg gaatccggac atcctcgaca aacgccggcg ccaggctcat gcccggcgct
      601 tcgggaatcg gctggccggc ccatgcccgc ggtttctcga cgcccgcaac cgacagcacg
      661 gtggggacga tgtcgatcac atgcgccggg gtgtggcgca aggcgttgcg ggcggtaatg
      721 cccttgggcc agtgggcgat gagcggggtg ctgatgcccc cctcgtgcac ccatgtcttg
      781 tggcggcggt acggcgtgtt gcacgcgctc gaaaaaccgg ggccgaggca aaggtaggtg
      841 gccgcgcagc ccggggcggc ctggggatca tgaccgccat cacggaccat gatctccgcg
      901 ctggccccat tgtcggacgc aaagaggatc agcgtgttgt cataggcccc catcgccttg
      961 agttgtccaa tgatccgccc gatctctcga tccatacgat ccaccatcgc ggcgtggatg
     1021 gccatcttcg tggcctggaa gcgccgctgt ttgccggtca actcgctcca gggcagggga
     1081 cgattcacct cgccggcccc cagtttctcg agagcctcgg gaaaatggta aggggggccg
     1141 acggcaggtt ccaggggcga cagggtggtc cggaccaggc ccatttccct ctggcgcgca
     1201 aaccggtcgg cacgcatggc ctcccagccc gcgaggtagc ggtcgcggta cttcgcgata
     1261 tcctccggca gtgcgtgcag cggaaagtgg ggcgcgataa aggcaatata gctgaaaaag
     1321 ggcttgccgg cgtgactcgt cgcgtggtcc ttcaggcagt tgatggcgtg atcggccgtg
     1381 gcgatggtcg cgtagtagcc ctgttcattc gccggcgact tcaggggcgc gtcgtcgatc
     1441 cggttgccgg ccgcggtgaa gtagtttccc tggttccgca tgtcgagcga acggtcgaag
     1501 ccgcccgcga gcaccccgcc gtccacgtgc cacttgccgc tgtgataact ccgatagccc
     1561 gcgggtttga ggaagtccgg cagcagccgc gcccagtgcg gacgcacccc tccagcgccg
     1621 cccccgagac cggggagcgc atcgcgcctg atctgctggg cgtagtagcc ggtgagcagc
     1681 gccccgcgcg tcggccagca acgggccgtg ttgtagaact gggtgaaccg cagcccctgc
     1741 ttcgccagcg tgtccagatg cggcgtctga atctcgcttc cgtagcagcc gagatcggag
     1801 aaccccagat catcggcgag gatgatcacg acgttgggtg gcgcgcccaa gcactgggtg
     1861 ctgaacgcag ccattgaaag aaagaccaac acgcgggcga acaacgcgct ggcgtttttc
     1921 ataatgcagc accttggacg attcatcgct tgcgggcaag aacctcgtcg tggacggcga
     1981 taaccaccgg catttgaagc gacggcgctg gacggagtcg gagttgggac gacgcgcgaa
     2041 aggggatgcg ggaaagctgg cgatcgccgt gcggttgcag gcggagacgg tgatggcggt
     2101 cgcaagatct ggtaactgcc aaactcgctg acggtgaaca cgatgtgccg cccgtcgggg
     2161 aacgggcggg gctgctgggc gctgaagcgg cgttccccat cggaaatgag ggtttcggga
     2221 ttgccccagc gattccggtc cgggtcgtag gggattggga cactgtcgta gcgaatggtg
     2281 cgcacctcgg ccaagggcag gtctggaccg ctgcaatagt agagagagag tccttcaggc
     2341 gcccagcatg ggcaattctc gtcacggtca ggcgcggcga tagcgggagg cttttcgatc
     2401 gtgcggtctc gcgcatgaag aatggcgagg tccgagcatt cagagtgacg aggtctcttc
     2461 gctgcttgcc atgcggtgct gagcagcgcg atcgcgaggg ggcagcggat caagcctgat
     2521 tgaggcgatc cgcgattcac gtcacaactg ctgcatcgcc tggtcgagag cggtgtagag
     2581 ggcctgcatg ctcgattcgg tttcgtcgcc catgttcgag atgcggaagg tggttccctt
     2641 gatcttgccg taaccgccat cgatgaggaa accctgatct ttgaccagct tttgcagctt
     2701 gggcacgtcg acggtcctgg atccgggctt ggccccgttg ttgacgcacg tgagcgtcat
     2761 tgattcgaag cccttgtccg gaaacaggtt gaatccgtgt cgggccgccc aatcgcgggt
     2821 catcccggcc agtttggcgt gccgggcaaa ccgggcatcg agtccttcgg cgaaaatgtc
     2881 ctcgagcttc gaggcgaggg catggacgtg gccgatgctc ggggtgctgg gggtcatgct
     2941 ctgcgcggcg ttcttctgga actcaaccag atcgaagtag tagccgcgat cctttgccgc
     3001 ggcggctttg gcgagcgctc cagccgagca ggtgaacacg gtcagtcccg gcgggagggc
     3061 aaaggccttc tgcgatccgg cgagcaacac atcgatgccc aaggcgtcga actcgatctt
     3121 gactgcgctc atcgaactga cggagtcgat gatgaacatc acgtcaggat acttggcctt
     3181 gagcgccgcc acttcggcca gcgggctcat gacgccagtc gacgtctcgt tgtgaatgaa
     3241 cgtgagcgca tcgaacgctc cggtcgccag tttgcggtcg acgtcctcgg tgcggatggg
     3301 cgagccccag gggacctgca gggcctcggc ctccttgccg cagcgtttcg agacgtcgtt
     3361 ccacttgtcg gaaaaggcgc cgcacatgca attgagcacc ttcttgtgca ccaggttgcg
     3421 aacagctccc tccatcaccc cccacgcgga ggacgtgctc aggaagacag gctgccgcgt
     3481 gtagagcaat tcctggagcc gcggctggat gctcgcgtac aggtctttga agccttggct
     3541 gcgatgtccg atcatgggcg aacagaacgc gcggaaggtc ttctcgctga cttcaaccgg
     3601 tcccggtatg tgcaatttga ggtgcgccat aaaaagaatg gccgcgaaat tttcacagcc
     3661 gatggccgat ggcaagccga gttttggagc agtcggcgac cgctcttcca taaaccgtaa
     3721 tcgcggacgt gagacagaca cgggtgtttc gagtgcattg cccccctgca cggcgcgctt
     3781 gtcatggaca tgcctgccag tctgtcctgc gtccacacgc acacaaactc agggctctcg
     3841 ggattccgcg gcagggctgg aactccggcc tcgatccggt ggaagccggg cgacccttct
     3901 cgagcgatcg actatcgatt atgggcacaa agccaactca acacagtccg ggactcctct
     3961 ggctgggggg gctgccgtcg ccatgctggt cgccgccacg ccgtccagcc tcgcgtcgat
     4021 tcgggcgcct tacacgcctg acgcgaacac caaactgctg ttgcacctcg atgacgccac
     4081 cgagagcggc atcgccgcca acgcagtggc cggggcgccg tcgttcatcg cgacggccaa
     4141 cccgtcggct gcgacgtcgc gccagccgat cccggggata ttgggagcgc ctggggcgtc
     4201 ggggggtgtg ggctaattgg gcagaaagca gttcccgcct cgagaacacc gagaaagggg
     4261 cttgactggc ggaggcggtc ggccttggga gtgcggcttt cgaggaaatc ggcaagttgg
     4321 ggcgcctgac aattcggctc gccaaccc
//
LOCUS       DAOQRO010000298         4315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21869_length_4315_cov_4.108216, whole genome shotgun sequence.
ACCESSION   DAOQRO010000298 DAOQRO010000000
VERSION     DAOQRO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21869_length_4315_cov_4.108216"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKK20_10635"
     CDS             <1..292
                     /locus_tag="PKK20_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV00384.1"
                     /translation="LLALAMGLARPQATEFSFMLGLPTLMAAAVKELMDFHETPSGFD
                     IDWSLVLIGSIASAVTAFLSVKWLLRFVQSHTFNGFGWYRIIVGGAILLFAW"
     gene            complement(275..1243)
                     /locus_tag="PKK20_10640"
     CDS             complement(275..1243)
                     /locus_tag="PKK20_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV00385.1"
                     /translation="MSIPSIRVLVTGGAGFIGSHLAQALCEQGAEVLVLDNLSSGLRA
                     NLDWRQSSHRLEFVEGDIRDEALVRRCVTGCHWVFHQAAIASVPYSVDHPAETNSQNL
                     TATLSLLEAARDAGVRRFVFASSSAIYGDGPDPVKHEGLPPLPLSPYALQKYASERYG
                     QLFHALYGIETVGLRYFNVFGPRQVFDSPYSGVIARFCTAFLEGQRPTIFGDGLQSRD
                     FVYVSDVALANIRAAMAPASRVAGRVFNIAGGRSVSLLDLVSSLNALTAQRLEPVFEP
                     ARAGDVRHSQADIAAAQAGFEFEASVSLSEGLESTLDFYRSSPGKQ"
     gene            complement(1321..2304)
                     /gene="cysK"
                     /locus_tag="PKK20_10645"
     CDS             complement(1321..2304)
                     /gene="cysK"
                     /locus_tag="PKK20_10645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNV00386.1"
                     /translation="MRQYYTDIVQTVGRTPLVRLNRLAAGLDATVLVKCEFFNPLGSV
                     KDRIGVAMIEDAERRGVLKPESVIIEPTSGNTGVALAFVAAAKGYKLILTMPDTMSLE
                     RRTLLAMLGARLVLTPGVEGMRGAIARAEQLAQETPKAWMPQQFENPANPDVHRRTTG
                     EEIWQDTDGLIDFFVASVGTGGTLTGCCEAIKPRRPGFQAIAVEPKDSPVISQTLAGQ
                     PLAPGPHKIQGTGAGFVPKNLRLKNAQGAPQITECIQVTNDDAFAMARRLAKEEGILA
                     GISSGANVWAALEVAARPENKGKLIVTVACSTGERYLSTPLAAEARASVGA"
     gene            2739..2885
                     /locus_tag="PKK20_10650"
     CDS             2739..2885
                     /locus_tag="PKK20_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00387.1"
                     /translation="MAPITGGDGRFDGEAKAYQPRLDLVHYRGSATFNYTTPERGMET
                     PDGE"
     gene            3066..>4315
                     /locus_tag="PKK20_10655"
     CDS             3066..>4315
                     /locus_tag="PKK20_10655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV00388.1"
                     /translation="MNIIQLTPGAGGMYCGNCLRDNALVAEYRRRGHTAIMVPLYLPL
                     TLDEPDLSAGTPVFFGGINVYLDHKTPLFRSAPAWLRRWLASPALLRWTGRFVAKTRA
                     RDVADLTLSMLRGEEGNQARNLDEFIVWLRAQPKPDVVCLSNALLIGAARRIRRDLGV
                     PVVCLLAGEDSFLDSMPEPWRAEIWKTLAARAADIELFVAPSRYFADRMRARLGIAAD
                     RIEVVPTAINLEGYPVPPAAHPQTSEAPQSTDQTSASAPDVARLTTGGPRIVPSGDTP
                     ILGYFARMCPEKGLDTLIDAFIAIRRRARVPTLRLKVGGGCGPTDEAFVEDMRARLRA
                     SGLLADVEFHPNLPRSEKIAFLRSLTVFSVPALYGEAFGMYLIEALASGVPVVQPNHA
                     AFPEIVQATGGGVVCAAGDHRALA"
BASE COUNT          743 a         1432 c         1352 g          788 t
ORIGIN      
        1 tctgttggcg ctggccatgg gtttggcgcg accacaggcg accgagttct cgttcatgct
       61 gggcttgccg accctcatgg ccgcggcggt taaggaactc atggacttcc atgaaacgcc
      121 gtccggcttc gacatcgact ggtcgctggt cctgatcggc tcgatcgcct cggcggtgac
      181 ggcgtttctt tccgtgaaat ggctgctccg cttcgtgcaa agccacactt tcaacgggtt
      241 cggatggtac cgaattatcg tgggcggtgc gatcctactg tttgcctggt gacgaccgat
      301 aaaaatcgag cgtggactcg agcccctcgc tgagtgagac gctcgcctcg aattcgaacc
      361 ccgcctgcgc cgccgcaatg tccgcctgcg aatgacgcac gtcgcccgcc cgggcgggct
      421 cgaacaccgg ctcgagccgc tgtgcggtca atgcgttcag actcgacacc agatccaaca
      481 acgacaccga gcgcccgccc gcgatgttga agacccggcc cgccacccgc gacgccgggg
      541 ccatcgccgc ccggatattc gccagggcca cgtcgctcac atagacgaaa tcgcgcgatt
      601 gcaggccgtc gccgaagatc gtcggtcgct gcccctcgag gaacgccgtg cagaaccgcg
      661 cgatcacccc cgagtacggc gaatcgaaca cctggcgcgg cccgaacacg ttgaaatacc
      721 gcaatccgac cgtctcgatt ccgtacaggg cgtggaagag ttgaccgtaa cgctcgctgg
      781 cgtacttctg cagcgcgtac ggagacaaag gcaatggcgg caggccctcg tgtttcaccg
      841 gatcgggacc gtcgccgtaa attgccgacg acgaggcaaa cacaaaccgg cgcaccccgg
      901 catcgcgcgc ggcctcgagc aggctgaggg tggcggtcag gttctggctg ttggtctcgg
      961 caggatggtc caccgagtat ggcacggacg cgatcgccgc ctgatgaaag acccaatgac
     1021 atcccgtcac gcacctgcgc accagggcct catcgcgtat atctccctcg acgaattcaa
     1081 ggcggtgtga gctttggcgc cagtccagat tggcgcgcag tccggaactg agattgtcga
     1141 gcacaagcac ttcggcgccc tgctcacaga gcgcctgcgc caggtgcgat ccgatgaatc
     1201 ccgcgccacc ggtcaccagc acgcgaatgg aaggaattga catcggaagg ctagcccgca
     1261 tagcagtcct cttgcgaagt ctgagtgctc tgtgcaccat cgaggtgtga aatatgtggg
     1321 ctaggccccg accgacgccc gcgcctcggc cgccagcggg gtgctcaggt aacgctcgcc
     1381 ggtcgaacag gccacagtca cgatgagttt acccttgttt tcaggacgcg cggcgacttc
     1441 gagcgcagcc cagacgttcg cgcctgacga aatcccggcc agaatcccct cctccttcgc
     1501 caaccgacgc gccatggcga acgcatcgtc attcgtgacc tggatgcatt cggtgatctg
     1561 aggcgctccc tgcgcgtttt tcaggcgcag gttcttgggc acgaatccgg cgccggtccc
     1621 ctgaatcttg tgcggacccg gcgcgagcgg ctggcccgcc agggtctgcg atatgaccgg
     1681 cgagtccttg ggctcgaccg caatggcctg gaaacccgga cgccgcggtt tgatggcctc
     1741 acagcagcct gtcagcgtgc cgcccgttcc cacgcttgcc acaaagaagt cgatcaggcc
     1801 gtcggtatcc tgccagattt cttcaccggt ggtcctgcga tggacgtccg gattggccgg
     1861 gttctcgaac tgctgcggca tccaggcttt gggcgtctcc tgcgccagtt gctccgcccg
     1921 cgcaatcgcc ccgcgcatgc cttcgactcc gggtgtcaat accaggcgcg ccccgagcat
     1981 cgccaacaag gtccgacgtt cgagggacat cgtgtcgggc atcgtcaata tgagcttgta
     2041 gcccttggcc gcggcgacaa aggcaagggc gacccccgtg ttgccgctcg tcggctcgat
     2101 gatcacgctc tcgggcttga gcacgcctcg acgctcggcg tcttcaatca tggccacccc
     2161 gatgcggtcc ttcacgctcc cgagcggatt gaagaactcg cacttcacca gaacggttgc
     2221 gtccagtccg gcggccaggc gattcaggcg caccagcggc gtgcgcccga cggtctggac
     2281 gatgtcggtg tagtattgcc tcataacatc cattagaatc gacagaaccc tcaccaatct
     2341 tgccgcgctg ctccgatgca gcaagaagat tcaatctggc agttcggtga tcggagttcg
     2401 gggatcggag atgggagcgc tcaagtatga acaacgtcgg ggtccccgcc tctcgatgac
     2461 ctcgttgccg cccgtgaggt ttcgccggtc atcgagcacc ctcccgatcg cggtcggtca
     2521 gcctccaaag acacacctcg aagacagacc gtatgcgggt ttggtgcttg cgcttcggcg
     2581 gggcgagccc gcgcaggccc gggtcaaggc gccgggtctt cgtgatgggc ggctggagac
     2641 gggagcggga tcgctggtcc ccttgggcgg cgtgtcgctg gagcaatcgt attgtcagtg
     2701 atggcgacgg agtttaggaa ccgaccgagt ccaactactt ggccccgatt accggcggcg
     2761 acgggcgatt cgatggcgag gccaaggcct atcaaccgcg ccttgatctc gtgcattatc
     2821 gaggctccgc cacgttcaac tacacgacgc ccgaacgcgg catggaaacc ccagatgggg
     2881 aatgaaatcc ccgtttgcta atcaaagcgg aaatcgcggt catcggaaga tgcgcgccgt
     2941 gtatctggcg atgcaccgag atacgcgccg cggcggtgca cgcgcttgcc tttcgccctg
     3001 ccgcgggcca gagtcgtccg catgatatcc cgaaacgcga agacgggctc gcttcggatc
     3061 ggactgtgaa catcatccag ctcaccccag gcgcgggcgg catgtactgc gggaactgcc
     3121 tgcgcgataa cgccctggtc gccgagtatc gccggcgcgg ccacaccgca atcatggtgc
     3181 cgctctacct gccgctgacc ctggacgagc ccgacctgag cgcggggacc cccgtcttct
     3241 ttggcggaat caatgtctac ctcgaccaca agacgccgct tttccgctca gcgcccgctt
     3301 ggctgcgccg gtggctggcc tcccctgccc tgctccgctg gacgggccgg ttcgttgcca
     3361 aaacgcgggc gcgtgacgtg gcggatttga cactgtcgat gctgcgcggc gaggaaggta
     3421 accaggcgcg caatctggat gagttcatcg tctggctgcg cgctcagccc aagccggatg
     3481 tggtctgtct gtccaatgcg ttgctgatcg gagccgctcg ccggatccga cgcgacctcg
     3541 gcgttccagt ggtctgcctg ctggccggcg aagacagttt tctcgacagc atgcccgagc
     3601 cgtggcgcgc cgagatctgg aagaccctcg ccgctcgggc ggctgacatc gagttgttcg
     3661 ttgccccaag ccgttacttc gcggatcgga tgcgcgcgcg tctggggata gcggcggacc
     3721 gcatcgaggt ggttccgact gcgatcaatc tcgaaggcta ccccgtcccg cctgccgccc
     3781 atccccagac ctccgaagcg ccgcagtcca cagaccagac gtccgcctcc gcgcctgatg
     3841 tcgcacggct cacgactggc gggccgcgga tcgtgccttc gggcgacacc cctatcctcg
     3901 gctacttcgc ccgcatgtgc ccggagaaag gcctcgacac gctgatcgac gcgttcatcg
     3961 cgatccgacg ccgcgcgcgt gtgccaacac tccggctgaa ggtgggcgga gggtgcgggc
     4021 cgaccgatga ggccttcgtc gaggatatgc gcgcgcgact gcgggccagc ggcctgctgg
     4081 ctgatgtcga gttccatccg aacctgcctc gctccgaaaa gatcgcattc ctgagatccc
     4141 tgaccgtgtt ttcggttccg gcgctctacg gcgaggcctt tggaatgtac ctgatcgagg
     4201 cactggcctc cggggtgccg gtggtgcagc ccaaccacgc ggcctttccc gagattgtcc
     4261 aggcgacggg cggcggtgtg gtctgtgccg cgggcgacca tcgggcccta gcgca
//
LOCUS       DAOQRO010000299         4307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21924_length_4307_cov_4.897460, whole genome shotgun sequence.
ACCESSION   DAOQRO010000299 DAOQRO010000000
VERSION     DAOQRO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21924_length_4307_cov_4.897460"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..531
                     /locus_tag="PKK20_10660"
     CDS             127..531
                     /locus_tag="PKK20_10660"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNV00389.1"
                     /translation="MRAVKVAAGLVFRDGKVLITQRHPADQWGGFWEFPGGKCEPDES
                     YPDALRRELREELDIEVTVVDRIECVDHPYADRTVHIEFFRCRWVTGEPRAVGCAQFV
                     WAGLDELDRYQFPTADTSLLARLRSMPDLWLE"
     gene            complement(551..991)
                     /gene="rplQ"
                     /locus_tag="PKK20_10665"
     CDS             complement(551..991)
                     /gene="rplQ"
                     /locus_tag="PKK20_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNV00390.1"
                     /translation="MRHLKRTAKLGRTSEHRKALLANLVCSLIKKRRITTTLAKARAA
                     RPVAEKMMTLAKGGTLHHRRLASSRLLQDEQAVRVLFNELAPAHRDRRGGYTRILKLN
                     QRQGDAASLAILEWVEGAPVATTSAGGSQPGAAAPDAAPPAKTK"
     gene            complement(1007..2020)
                     /locus_tag="PKK20_10670"
     CDS             complement(1007..2020)
                     /locus_tag="PKK20_10670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNV00391.1"
                     /translation="MPVRLGRFEMPKRLSKEEATATATYAKFIAEPFETGYGHTIGNS
                     LRRVLLSSLEGAAITSVKIAGAQHEFTVVDGVVEDVTDIVLNLKKVKFKAHSREPQTL
                     LLSVNKEGPVTAANIQLNQHVELVNPSQLICTIDRKKKFEMEIEVKVGRGFCPGDENK
                     KVDQAIGVIAIDSLFSPVERVRYAVENARVGQKTDYDRLLLEIWTDGRISPDDALVQT
                     SAILQRHLDVFVGYDKNAIEFEEVTDSQDEERTRMRKLLNMSVNEIELSVRAANCLNN
                     ANITTVGQLAMKSEQEMLKYRNFGKKSLNEIKEKLQALNLTLGMNLDAALLDPPKDEA
                     RKS"
     gene            complement(2132..2740)
                     /gene="rpsD"
                     /locus_tag="PKK20_10675"
     CDS             complement(2132..2740)
                     /gene="rpsD"
                     /locus_tag="PKK20_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNV00392.1"
                     /translation="MARYTGPRVRISRRFGTPIFGASKYLERRNYGPGVHGPKSRRKV
                     TDYGQGLIEKQKLRYYYGLLERQFRGVYERALRKRGVTGETMLQILETRLDSIVYHLG
                     FANTRASARQMVSHGHIRVNGRKVGIPSSAVRVNDVIEVRDRSVSRQMATKNLESATS
                     RAVPDWVSLNREAFRGTMVRMPTREDIQPIANEQAVVEFYSR"
     gene            complement(2785..3435)
                     /gene="rpsK"
                     /locus_tag="PKK20_10680"
     CDS             complement(2785..3435)
                     /gene="rpsK"
                     /locus_tag="PKK20_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNV00393.1"
                     /translation="MAEETKPQKATVKKEAKSEPAASEKAAVKPSAPAKGAAKPDAEA
                     GKTEAPKAARAAAAAPDQKAAGAPAPGGPVAGIAPTAADLLADEMAGKKIVRAKGAKN
                     ISAGVAHILATFNNTQVSITDMQGNLIGWSTAGKVGFRGSRKSTAFAAQQVAQDAARQ
                     AMSHGMREVEVHVKGPGSGRESAIRALQAIGLEISVIRDRTPIPHNGCRPRKKRRV"
     gene            complement(3459..3854)
                     /gene="rpsM"
                     /locus_tag="PKK20_10685"
     CDS             complement(3459..3854)
                     /gene="rpsM"
                     /locus_tag="PKK20_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNV00394.1"
                     /translation="MARILGVDIPGEKRIDIALRYVYGIGPSNAKQILEHAQIDPAVR
                     AKNLTEQQLSQIVHAIQEGEYVIEGDLRREIGLNLKRLQGIKCYRGMRHIRGLPVRGQ
                     RTQTNARTRKGPRKTVGVVRNPKAKTGIH"
     gene            complement(3870..3983)
                     /gene="rpmJ"
                     /locus_tag="PKK20_10690"
     CDS             complement(3870..3983)
                     /gene="rpmJ"
                     /locus_tag="PKK20_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNV00395.1"
                     /translation="MKVRSSVKKLCEHCKVVRRKGVVRVICTNARHKQRQG"
     gene            complement(3980..4207)
                     /locus_tag="PKK20_10695"
     CDS             complement(3980..4207)
                     /locus_tag="PKK20_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00396.1"
                     /translation="MLSRDTIEAFAVIVASGGGVPVRARLANGHRFVVCATRSARRAV
                     AGLAVGDRIRARFSPCDLSKGWYIVDSEGKQ"
     gene            complement(4171..>4307)
                     /locus_tag="PKK20_10700"
     CDS             complement(4171..>4307)
                     /locus_tag="PKK20_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNV00397.1"
                     /translation="LSDGWTVVSEDGLPSAHFEHTVLVGEGPAEILTCCPETPLRPLP
                     "
BASE COUNT          718 a         1355 c         1337 g          897 t
ORIGIN      
        1 cacccgtcgc gtccgcgaca acgacggtta cagccgcgcc ggcgacaccg ccatccaacg
       61 ccgtgtccac ggtcgagacc tcgacaccgg ctgcgacgac cgaggccgcc ccggtcgagt
      121 agttttgtga gggccgttaa ggttgcggcg gggctggtct ttcgggacgg aaaggtcctc
      181 attacacaac gccaccccgc ggatcagtgg ggcggttttt gggagttccc cgggggcaaa
      241 tgcgagcctg acgagtctta tccggacgcc ctgcgccgtg aacttcgcga ggaactcgac
      301 atcgaggtga cggtcgtgga tcgaatcgag tgcgtcgacc atccttacgc cgaccggacg
      361 gttcacatcg agttcttccg atgccgatgg gtaaccggcg aaccgcgcgc tgtggggtgt
      421 gcccagtttg tgtgggccgg cctggacgaa ctcgaccggt atcaattccc cacagcggac
      481 acaagcctcc tggcccgact ccggtcgatg cctgatctct ggctcgagtg actccgcgga
      541 acgatgcccg ctacttggtt ttcgccggag gggcggcgtc tggggcagcc gcgccgggct
      601 ggcttccacc cgcgctggtc gtcgcgacgg gcgcgccttc gacccattcg aggatggcca
      661 ggctggcggc atcgccctgg cgttggttga gcttaaggat gcgggtgtag ccgcctcgcc
      721 gatcgcggtg ggcgggagcg agttcgttga acagaacgcg cacggcctgt tcatcctgga
      781 gcaggcggct cgaggccagg cgtcggtggt ggagcgtgcc ccctttggcc agggtcatca
      841 tcttctcggc cacgggccgt gcggcgcgcg ccttggcgag ggtggtggta atccgtcgtt
      901 tcttgatcag actgcacacc aggttggcca gcagggcctt tcgatgctcg ctggtgcgtc
      961 cgagtttggc ggtgcgtttc aggtgtcgca taggggcggg agaaggttag gatttgcgcg
     1021 cctcgtcctt gggcggatcg aggagggcgg cgtccaggtt catgcccagg gtcaggttga
     1081 gggcctggag tttctctttg atttcgttga gcgatttctt gccgaaattg cgatacttga
     1141 gcatttcctg ttcggacttc atagccaact gcccaacggt ggtgatgttg gcattgttga
     1201 ggcagttcgc cgcgcgcacg ctcaactcga tctcattgac gctcatgttc agcagcttgc
     1261 gcattcgcgt ccgctcctca tcctgactgt ccgtcacttc ctcgaattcg atcgcgttct
     1321 tatcgtaacc gacaaacaca tcgaggtggc gctggagaat ggccgaggtc tgcaccagcg
     1381 catcatcggg cgagatccgt ccgtcggtcc agatctcgag cagaaggcgg tcgtaatcgg
     1441 tcttttgccc gacgcgcgcg ttttcgacag cgtagcggac ccgctcgacc ggggagaaaa
     1501 gcgaatcgat ggcgatcacg ccgatggcct ggtcgacctt cttgttctca tcgcccggac
     1561 aaaacccgcg tccgaccttc acctcgattt ccatctcgaa tttctttttg cgatcgatgg
     1621 tgcagatgag ctgggaagga ttgaccagct cgacatgctg gttgagttgg atgttggcgg
     1681 cggtcaccgg gccttccttg ttcacggaga gcaggagcgt ctgcggctcg cggctgtgcg
     1741 ccttgaactt gaccttcttc aggttcagga cgatgtcggt cacatcctcg acaaccccat
     1801 cgacgacggt gaactcgtgc tgggccccgg cgatcttgac cgaggtgatc gccgccccct
     1861 cgagcgagga gaggagcaca cggcgcagcg agttgccgat ggtgtggccg tagccggttt
     1921 caaacggctc ggcgatgaac ttggcatatg tcgccgtcgc agtggcctct tccttcgaca
     1981 accgcttcgg catttcaaac cgacctagac gtacaggcat ggctggatat gcgacaattt
     2041 taaactgatc cgcccgcgat cttcccgcaa tccgggcgaa tccatataat tgcgtccggc
     2101 atcccacgcg ggagccggca ggaactcgaa cctatcggga ataaaactcg accaccgcct
     2161 gctcgttggc aatgggctgg atgtcctccc gggtgggcat gcgcaccatc gtcccccgaa
     2221 aggcctcgcg attcagcgag acccagtcgg gtacggcccg cgacgtggcg gactcgaggt
     2281 tcttggtggc catctgccgg gacacgctcc ggtcccgcac ctcgatgaca tcgttgacac
     2341 gcacggcgct cgatggaatc ccgaccttgc ggccattgac ccggatgtgc ccatggctga
     2401 ccatctgccg ggccgacgcc cgggtgttgg cgaacccgag gtgatagacg atgctgtcca
     2461 gacgcgtctc gagaatctgg agcatcgtct cgcccgtgac accgcgcttg cgcaacgccc
     2521 gctcatagac ccctcgaaac tggcgttcga gcaacccgta gtaatagcgc agcttttgct
     2581 tctcgatgag accctgaccg taatcggtca ccttgcggcg cgatttcgga ccgtgaacgc
     2641 cggggccata attgcggcgc tcgaggtact tcgaggcgcc aaagatcggg gtgccgaaac
     2701 ggcgactaat gcgaacgcgc ggtccagtgt agcgagccat gaattaaagc ggatttgagg
     2761 ttcgagattc gagaattgag ggtgtcacac acgacgcttc ttgcgggggc ggcatccgtt
     2821 gtggggaatc ggcgtccggt ccctgatcac actgatctcg aggccgattg cctgcagagc
     2881 ccggatcgct gattcgcgtc cggagccggg gcccttcaca tgcacctcga cctcccgcat
     2941 gccgtgcgac atcgcctgac gggccgcgtc ctgggcaacc tgctgggcgg caaaagccgt
     3001 gctcttccgc gacccgcgga atccgacttt gcccgcggtc gaccatccga tcaggttccc
     3061 ctgcatgtcg gtgatgctca cctgggtgtt gttgaacgtg gccaggatat gcgcaacgcc
     3121 cgcggaaatg ttcttcgccc ctttggcccg gacaatcttc ttgccggcca tctcgtccgc
     3181 cagcagatcc gccgccgtcg gagcgatgcc cgccaccggg cctcccggcg caggcgcgcc
     3241 cgctgccttt tgatccggcg cggcggcggc cgctctcgcg gctttgggcg cctcggtctt
     3301 gcccgcttcg gcatcgggct tcgctgcccc tttggccggc gcagacggct tgacagccgc
     3361 cttctccgag gctgcgggct cggacttggc ctctttcttg acagtggctt tttgcggttt
     3421 ggtctcttca gccatagcaa tgactttgga tcagtgggtt agtgaatgcc ggtcttggcc
     3481 ttcggattgc ggaccacgcc gacggtcttg cgcggtccct tgcgcgtccg cgcgtttgtc
     3541 tgagtacgct ggccgcggac cggcaagccg cggatgtgcc gcatcccgcg gtagcacttg
     3601 atgccctgca ggcgcttgag gttcaagccg atctcccgcc gcaggtcgcc ctcgatcacg
     3661 tactcgcctt cctggatcgc gtggacgatt tgcgaaagct gctgttcggt caggttcttg
     3721 gcgcgcacgg cggggtcaat ctgggcgtgc tccagaatct gtttcgcgtt cgacggtccg
     3781 atgccgtaaa cgtaccggag ggcgatgtcg atccgcttct ctccgggaat atccactccg
     3841 agaattcgtg ccatagagcg ctttgcagtt taaccttgcc gttgtttgtg ccgggcgttg
     3901 gtacaaatga ctcgcaccac gcccttgcga cgcaccactt tgcagtgctc gcagagcttt
     3961 ttgaccgatg aacgtacttt cattgctttc cttcgctgtc gactatgtac cagcccttcg
     4021 acaaatcaca gggcgaaaac cgcgcccgaa tccggtcgcc aaccgccagg ccggcaaccg
     4081 cgcgccgtgc cgaccgcgtt gcgcagacca caaaccgatg cccattcgcc agccgagccc
     4141 gaacgggcac cccgccgccc gaggccacaa tcacggcaaa ggcctcaatg gtgtctctgg
     4201 acaacatgtc agaatctcgg ccggtccttc gcccaccaga acagtatgct cgaaatgggc
     4261 agaagggagg ccatcttcgc tgaccacagt ccatccgtca ctcaaaa
//
LOCUS       DAOQRO010000300         4303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_21951_length_4303_cov_6.645245, whole genome shotgun sequence.
ACCESSION   DAOQRO010000300 DAOQRO010000000
VERSION     DAOQRO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21951_length_4303_cov_6.645245"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..720)
                     /gene="tsaB"
                     /locus_tag="PKK20_10705"
     CDS             complement(58..720)
                     /gene="tsaB"
                     /locus_tag="PKK20_10705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNV00398.1"
                     /translation="MKILALEFSTGHRTVAALDTEAMDNARAPAIEIEEVQGRSVRAC
                     AMVESALRKAGLARESVDTVAVGLGPGSYAGIRTAIAFALGWHMARTVRLAGISSIEC
                     LVAGLESRGTSGSLLAAVDAQRGEFYLAGYELGPTGWREVEPLHLASGEELERRVRAG
                     ARVVGPDIPSHIRGVQRVFPDAAILARLAAGRAGLERGARLEPIHLRPTRFVKAPPPR
                     AV"
     gene            complement(701..1153)
                     /gene="tsaE"
                     /locus_tag="PKK20_10710"
     CDS             complement(701..1153)
                     /gene="tsaE"
                     /locus_tag="PKK20_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNV00399.1"
                     /translation="MVTFTSRSADETVSLGEAWGRQARRGWVIGLSGELGVGKTQLVK
                     GLARGLGATERVHSPTFTLVNEYASGRLALFHLDLYRLETWAQFESAGLDEYLYQPPG
                     VTVIEWIDRCDAWTGSGLLAPPEPPFRRVWIETVDDTSRRIRYEDTGA"
     gene            complement(1131..2063)
                     /locus_tag="PKK20_10715"
     CDS             complement(1131..2063)
                     /locus_tag="PKK20_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNV00400.1"
                     /translation="MNEFELIRRLTHDLPRHPRVVVGVGDDCAVLDLGLPDRWVLFKT
                     DAVVEGVHFDAGALPEQIGHKALARCLSDVAAMGGEASSALITLGLPTAFDVARIEGV
                     YRGLNALAARHGVAIVGGETTTNPDRLLLSVCLLGSVEKTRCVTRSGAQPGDALFVSG
                     VLGGSLRGRHLCFEPRLAEARWLTGHFAVHAMIDLSDGLAGDLRHLLESSRVGAELLA
                     AAIPISREAKRAARERPDAKSALSAALTDGEDFELLFSVSSRDAVPLLDAWRERFPDL
                     GLACIGKILAERGVRLRDERGVRPLEAHGYIHFA"
     gene            complement(2060..2773)
                     /locus_tag="PKK20_10720"
     CDS             complement(2060..2773)
                     /locus_tag="PKK20_10720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNV00401.1"
                     /translation="MLPRQDWKLESVLRLFMILFLGLATVGLASNLADRLAGGEPDEA
                     RQALVGLIGTMVFQVVALGAVGWFVRENRTTWGEAFGLREDRLAWLALVAVAATVAVF
                     PISLALMWVSQKVMMWLSLEVVAQPTVRALEAVNSIGQKLLFGAMAVLLAPVVEEVVF
                     RGILYPTIKGLGYRRAALWLTAFVFALTHANAVTFLSLVFLAVLLTLLYEQTGSLWAP
                     IGAHGLFNLANFTWLLLGR"
     gene            complement(2781..3917)
                     /gene="tgt"
                     /locus_tag="PKK20_10725"
     CDS             complement(2781..3917)
                     /gene="tgt"
                     /locus_tag="PKK20_10725"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV00402.1"
                     /translation="MFEIVQTDPASKARLGRLTTARGIIDTPVFMPVGTQASVKALDP
                     RELREMGTQIILANTYHLSIRPGLDIIEAAGGLHRFMAWDGPILTDSGGFQVFSLAKI
                     RKVKAHGVEFRSHLDGSPLFLGPKEAMAIQRALGSDIAMVFDECPPHDAPAKEQRLAV
                     ERTIRWAGECREQPRSPGQLVFGIVQGGSNAALREACALAITALDFDGYAIGGVSVGE
                     PEPEMLRAIEFAEPFLPAGKARYAMGLGTPAQMVELVARGVDMFDCVLPTRVARNGTA
                     FTRRGTFAIKGGACKAEVRPIDEICDCFACRHFSRAYIRHLLNVNEILGLRLVSIHNS
                     HFYLHVMREIRAHLAAGTFSDYRREFLAHYVPTRRVLASRHTEG"
     gene            complement(4027..>4303)
                     /locus_tag="PKK20_10730"
     CDS             complement(4027..>4303)
                     /locus_tag="PKK20_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00403.1"
                     /translation="ANTWVLIDEDADSLNDAAFAVGCLTPRWIDWPGTYHNMACGFAF
                     ADGHSEIHRWRQPDTKVYRGNTAQITPKNPSDWQWIAERTSARIPGK"
BASE COUNT          824 a         1499 c         1327 g          653 t
ORIGIN      
        1 ccaaatccct ttccagatac tcgtcaagca ccgctccaaa tgaaaagacc gtgcgcttca
       61 cacagcccgc ggcggcggcg ccttgacgaa acgggtcggg cgcaaatgga tcggttcgag
      121 gcgggcccct ctctcaagcc ccgcgcggcc agccgccagc cgcgcaagga tcgccgcgtc
      181 cggaaagacc cgctgcacac cgcggatgtg ggacgggatg tctgggccga caacgcgggc
      241 gccagcgcga acccgccgtt caagttcctc gccactggcc aaatgcagcg gctcgacctc
      301 gcgccagccc gttggcccga gctcgtagcc cgcaaggtag aactcgccgc gctgcgcatc
      361 gaccgcggcc aaaagcgatc ctgaggtgcc gcgcgactcg agccccgcga cgagacactc
      421 gatactcgag atgccagcca agcgaacggt gcgcgccatg tgccacccga gcgcgaatgc
      481 gatggcagtc cgaatgcccg cataactgcc cggccccagt ccaaccgcca cggtatcgac
      541 cgactcgcgc gcaagcccgg ccttgcgcag ggcggactcg accatggcgc aggcgcgaac
      601 ggatcggcct tggacctcct cgatctcgat ggcgggcgcc cgcgcattgt ccatggcctc
      661 cgtgtcgagg gctgccaccg tgcggtggcc ggtcgagaac tcaagcgcca gtatcttcat
      721 aacgaatgcg ccggcttgta tcatcaaccg tttcgatcca gacacgcctg aacggcggct
      781 ctggcggggc caaaaggccc gatccggtcc aggcatcaca tcggtcaatc cactcgatga
      841 cggtgacgcc cggtggctgg taaaggtatt catcgagacc ggcgctttcg aactgcgccc
      901 acgtctcgag ccgatacaaa tccaggtgga acaaggccag ccgcccgctg gcgtactcgt
      961 tgacgagcgt gaacgtcggc gaatgcaccc gctcggtcgc gccaagcccc cgggcgaggc
     1021 ccttcaccag ttgggtctta cccaccccca gttcgcccga caacccgatc acccaacccc
     1081 ggcgcgcctg gcgtccccac gcctccccca ggctgacggt ctcgtccgcg ctacgcgaag
     1141 tgaatgtaac catgggcttc cagaggccgc actccgcgtt catcccgcaa ccgcactccg
     1201 cgctccgcga gaatcttccc gatgcacgcc agccccagat cggggaaacg ctccctccag
     1261 gcatcgagca gcggtacggc gtcgcggctc gaaacgctga aaagcaactc gaaatcctcg
     1321 ccgtcggtca gtgcggccga caaggcggac ttggcgtcgg gacgttcgcg cgcggcgcgc
     1381 ttcgcctcgc ggctgattgg gatcgccgca gccagcaact cggcgcccac ccggctggat
     1441 tcgagcagat gccgcaagtc gcccgccagc ccatcgctca agtcgatcat cgcatgcacc
     1501 gcgaagtgcc cggtcagcca gcgcgcctcg gccagacgcg gctcgaaaca gaggtgccgg
     1561 ccccgcaacg aaccgcccaa cacaccgctc acgaacaagg cgtcgccggg ctgggccccg
     1621 gatcgagtga cgcagcgggt tttctcgacc gagcccagca ggcagaccga gagcaggagc
     1681 cgatcggggt tggtcgtggt ttcacctccc acaatcgcca cgccatgccg cgcggccagg
     1741 gcgttcaacc cccggtaaac accctcgatg cgggcgacat cgaatgccgt cggcagccca
     1801 agggtaatga gcgcgctcga cgcttcgccg cccatggcgg ccacgtcgct caaacaccgc
     1861 gccagggcct tgtgcccgat ctgctcgggc agggcgcccg cgtcgaagtg gaccccctcg
     1921 accaccgcat cggtcttgaa cagcacccag cggtccggca acccgaggtc gagaaccgcg
     1981 cagtcatcgc ccacgccgac caccactcga gggtggcgcg gcaggtcgtg cgtcaggcgc
     2041 ctgatcaatt cgaattcgtt catctcccga gaagcagcca tgtgaaattg gccagattaa
     2101 acaggccgtg ggcgccaatg ggcgcccaga gcgagccggt ctgttcgtac aagagcgtca
     2161 gcagcacggc caaaaacacc aaagacagaa acgtcaccgc attggcgtga gtcaaggcaa
     2221 acacaaacgc cgtcagccac agggccgcgc gccggtatcc gagtcccttg atggtcggat
     2281 agagaattcc gcggaacacc acttcctcga ccaccggggc cagcaggacc gccatcgccc
     2341 caaacaggag cttctgcccg atcgagttca cggcctcgag cgcccgcacg gtgggttgcg
     2401 ccacgacctc gagtgacagc cacatcatga ctttctgcga cacccacatc agcgccaaac
     2461 tgatggggaa cacggccacg gtggcagcga ccgccaccag ggccagccag gctaagcggt
     2521 cttccctcag accgaatgcc tcgccccagg tggtccggtt ctcccgcaca aaccatccaa
     2581 cggcgcccag cgcgacgact tgaaacacca tcgtcccgat cagcccgacc agggcttgac
     2641 gcgcctcgtc gggttcccct ccggccagtc ggtccgcaag gttgctcgcc aaccccaccg
     2701 tggccagccc gagaaacagg atcatgaaca ggcggaggac cgactccagt ttccagtctt
     2761 ggcggggcag catagaccgg tcagccttcc gtgtgccggg acgccaggac gcgtcgggtc
     2821 gggacgtagt gggcgaggaa ctcgcgccgg tagtcgctga acgttccggc ggccaagtgt
     2881 gcgcggattt cgcgcatcac gtggagatag aagtgcgagt tgtggatgct gaccagccgc
     2941 aggccgagga tttcgttcac gttgagcaga tggcggatgt aggcccggct gaagtgcctg
     3001 caggcaaaac aatcgcaaat ctcgtcgatc gggcgcacct ccgccttgca ggcgccaccc
     3061 ttgatcgcga aggtgccgcg tcgcgtgaat gcggtgccgt tccgggccac gcgcgtcggc
     3121 aacacacagt cgaacatgtc cacaccgcgc gcgaccaact cgaccatctg cgccggggtg
     3181 cccaggccca ttgcgtaccg cgccttcccg gcgggaagaa aaggctcggc gaactcgatg
     3241 gcccgcagca tctccggctc gggttcgccc acgctgaccc cgccgatggc gtatccatcg
     3301 aaatccaggg cagtgatcgc tagggcacat gcctcgcgca atgccgcatt cgagcctccc
     3361 tggacgatgc caaagaccag ttgcccgggg gaccgcggct gctcgcggca ctcacccgcc
     3421 cagcgaatcg tgcgctcgac agccaggcgt tgttccttgg ccggcgcatc gtgcggcggg
     3481 cattcgtcga agaccatcgc gatgtccgag ccgagcgcgc gctggatcgc catcgcctcc
     3541 ttaggcccga ggaacagggg cgagccatcg aggtgagagc ggaactcgac gccatgggcc
     3601 ttcaccttgc gaatcttggc caggctgaac acctggaaac cgccgctgtc ggtcagaatc
     3661 ggtccgtccc aagccatgaa ccgatgcagg ccgcccgcgg cctcgatgat atcgagtccg
     3721 ggccggatgc tgaggtgata ggtgttggcg aggatgatct gggtgcccat ctcgcgcaac
     3781 tcgcgcgggt cgagcgcctt cacgctggct tgcgtgccga ccggcatgaa caccggggtg
     3841 tcgatgatcc cgcgggcggt cgtcaaacgc ccgagcctgg ccttcgaggc cggatcggtc
     3901 tggacgatct caaacacggg gccgatcaag cccgaaaacg gcgcgcgtca caagctttca
     3961 gaacaaaacg ccggacggtc cacgggccgt ccggcgtgct gtggaggcga atgtcaggaa
     4021 gtccggttat ttgcccggga ttctggctga agtccgttcg gcgatccact gccagtcgga
     4081 cggattcttg ggtgtgatct gggcggtgtt gccccgataa accttggtat ccggttgccg
     4141 ccatcggtgg atctccgaat gaccatcggc gaatgcaaag ccgcaggcca tgttgtggta
     4201 cgtgcccggc caatcgatcc atcgcggcgt caggcacccc acggcgaacg cggcgtcatt
     4261 caaactgtct gcatcctcat cgatgagaac ccatgtgttg gcc
//
LOCUS       DAOQRO010000301         4296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22016_length_4296_cov_5.755246, whole genome shotgun sequence.
ACCESSION   DAOQRO010000301 DAOQRO010000000
VERSION     DAOQRO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22016_length_4296_cov_5.755246"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1640
                     /locus_tag="PKK20_10735"
     CDS             243..1640
                     /locus_tag="PKK20_10735"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNV00404.1"
                     /translation="MNAIVKSILILPLAATLAVVRAAELRVGAAAVDITPPLGIPMAG
                     YYHARGADGVLDPLLSKAMVIEADGARAAFVALDLISVTREVTDQSRAEIEKSTGIPG
                     AHVMISATHAHTGPELAGRGKRSGDMGGQRQLSVDYTEGLPAKIAESVRLANERLEPA
                     QLRAAKGLCDDLTFNRRYYLRDGTVGWNPGKLNPNIVMPAGPSDDEVGILYAEKSGAT
                     GPAQAIATCVNFAMHADTTGGSKIGSDWPGALSRVLDGYHGTNHLSLVTLGTCGNLNH
                     LDFGWDWPQGTPIEPHRIGTVLGASVFRAYKQLQPVAAGPLRVLSETVELALPEVTPA
                     QVEEARQTLAVIKDDRGANFMKQVRAYRVLDIAERAGRPYRVEVQAVALGADVAWVSL
                     PGEVFVELGLAIKKRSPFSHTYVVELANENIGYIPDRRSYAEGNYEPESARCAAGSGE
                     ALVEAAVRLLTALRQ"
     gene            complement(1895..2494)
                     /locus_tag="PKK20_10740"
     CDS             complement(1895..2494)
                     /locus_tag="PKK20_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00405.1"
                     /translation="MPGLRVEITGIGPRKAREAALLALEKSRPTLVLSCGFAGGLDPS
                     LPRGTVLCSVDDGFPLESALIEAEAIHGRFVCSDRIVVTAAEKAALWQQTGADAVEME
                     SGVIRAICRERGVPSATVRVISDAADEDLPLDFNRLSGPNFELSYARLAAELLRRPGK
                     ALELLRFRRVLRQAACCLAPPLVKALSDNSRTQAHPGSG"
     gene            complement(2569..3693)
                     /locus_tag="PKK20_10745"
     CDS             complement(2569..3693)
                     /locus_tag="PKK20_10745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV00406.1"
                     /translation="MSGLADVIRAEIEAAGPISFARFMDLALYHPEFGYYERRSPVGR
                     HGDYYTSVSAGGLFGELLAFAFAQWGAGLNDGPLQIVESGAHDGRLARDIVDWFRRRG
                     GGRSQIEYWILEPSIARRAWQKETLGPCAASVRWASDWDSLPDCGVQGVIFANELLDA
                     MPVRRWAWDAAAMNWREWGVGRIGDAFAWVRLDRPQSTGPDSSPTGMSDPNEFFQSDA
                     SLLAVLPDGFIVETCPAALTWWRNAATRLRRGWLLTFDYGLTFEELLSPSRSQGTLRA
                     YHSHQVSNAVLDRPGEQDLTAHVNFSALRAAGEAAGLKTEHFESQGSFLVRVVGEIER
                     QSGRFGDWTPGRRRQLKTLIHPEHLGHAFRVLAQSRQARL"
BASE COUNT          785 a         1370 c         1352 g          789 t
ORIGIN      
        1 acggagcgag atggcgcgga ctcacgtccg cggctacgga accacggttc atggggagtt
       61 cccgtgtttc acgacccctc gattgcagca caaaagtgtg acttgtttac aacaagttcc
      121 accggtcaag gattccagat gcgtccccat tgcctcgttg ccaacgatcg gaactgagtt
      181 gcgggccttg attgggtggg cgagttggga caacaatgcg tgagacacaa ttcaatcgag
      241 tcatgaatgc gatcgtcaaa tcaatcctga tcctgcctct ggctgccacc ctcgcagtcg
      301 tgcgcgccgc cgaacttcgt gtcggcgcgg cggccgtcga catcacgccg ccgctcggca
      361 tcccgatggc aggttattac catgcgcgcg gtgcggatgg cgtcctcgac ccgctgttga
      421 gcaaggccat ggtcatcgaa gcggacggcg ctcgggcagc cttcgtggca ctggatctga
      481 tttccgtgac gcgcgaggtc acggaccagt cccgcgcgga gatcgagaag tcaaccggca
      541 taccgggggc gcatgtgatg atctcagcca cccacgccca tacggggccc gagctggccg
      601 ggaggggcaa gcgaagcggg gatatgggcg ggcagcggca attatcggtc gattacaccg
      661 agggtttgcc ggccaagatc gccgagagcg tccgtctggc caatgagcgt ctcgagcccg
      721 ctcaactccg cgcagccaag gggctttgcg acgatctgac tttcaaccgc cgctattacc
      781 tgcgcgatgg gacggtgggg tggaatcccg ggaagctgaa cccgaacatt gtgatgcctg
      841 ccggcccgag cgacgacgag gtggggatac tctatgccga gaaatccggt gcgaccggac
      901 cggcgcaggc catcgcgacc tgtgtgaact tcgccatgca cgccgacacc accggcggca
      961 gcaagatcgg atccgattgg ccgggggcct tgagccgggt gctggacggt tatcacggca
     1021 cgaatcatct cagcctggtc acactcggca catgcggcaa tttgaatcat ctggactttg
     1081 gctgggactg gccgcaaggc accccgatcg agccgcatcg gatcgggacg gtgctgggcg
     1141 cgtccgtctt tcgagcctac aagcagcttc aaccggtcgc cgccgggccg ctgcgcgtgc
     1201 tatccgagac ggtggaactc gcgttgcctg aggtgacacc cgcccaggtc gaggaagctc
     1261 ggcagacatt ggccgtcatc aaggacgatc gaggcgccaa tttcatgaag caggtgcggg
     1321 cctatcgcgt gctggacatt gccgaacgcg cgggccggcc ctatcgggtc gaggtgcagg
     1381 cggtggcgct gggcgccgac gtggcctggg tcagcctgcc gggcgaggtc tttgtcgagt
     1441 tggggttggc catcaagaaa cggtctccgt tctcccacac ctacgtggtc gagttggcca
     1501 acgagaacat cggctacatt cccgaccgcc gcagctacgc cgagggcaac tacgaacccg
     1561 agagcgcccg gtgcgccgcc gggtccgggg aggccttggt cgaggcggcc gttcgcctgc
     1621 tgaccgccct gcggcaatag cccgtatatt gtcccgcacc atgtcgcgac ccctttgtgg
     1681 caaagctccc acactggcgc gcgcacattc tcatcgaatc attcgaagac ggggcagtcg
     1741 gccactccgc attcatgagt gatggaccgc gccgccaggg gcaatccgcg gcctgcgtgc
     1801 cttgaggcgt cgccgctcca cttcatgaat tggccctggc tgtcagttgt ggctcaattc
     1861 ggttgcggct ttgccgcgct ggctaatatg cgggttaacc ggagcccggg tgggcctgtg
     1921 tgcgggagtt gtcgctcagg gcttttacca gtggaggggc caggcaacat gcggcctggc
     1981 ggagaacccg ccgaaatctg agcagttcga gcgccttgcc cgggcgacgc aacagttcgg
     2041 ccgccaatct ggcataactg agttcgaagt tcgggcccga gaggcggttg aagtccagag
     2101 gcaggtcctc gtcggcagcg tctgaaatca cccggacggt cgcactgggc acgcctctct
     2161 cccggcagat tgcccggatc acgccggact ccatctcgac tgcgtctgcc cctgtctgct
     2221 gccacagcgc ggccttttcg gcggcggtta cgacaatacg atcggaacag acaaagcgtc
     2281 cgtgaatggc ttcggcctcg atcagggcgg attcgagagg gaagccgtca tcgacggagc
     2341 agagcactgt gccgcgcggg aggctggggt cgagcccgcc cgcaaagccg cagctcaaga
     2401 cgagcgtcgg gcggctcttt tccagggcca gaagcgccgc ctcacgcgcc ttgcgcggtc
     2461 cgatgccggt gatttcgacg cgcagaccag gcatcccacg cgccgtccga aggaagggtt
     2521 tagcttcctc agcgacagca aacaggcaga gccgttggcc cacggtgttc ataatcgcgc
     2581 ttggcggctc tgggccagga cgcggaaggc atgtccgagg tgctcggggt ggatcagcgt
     2641 tttcaactgg cgccgccggc cgggtgtcca gtccccaaac cgccccgact gacgctcgat
     2701 ttcgccaacc acacggacca ggaaactgcc ctgactctcg aaatgctcgg tcttcaagcc
     2761 ggcggcttcc ccggcggcgc gaagggccga gaaattgacg tgcgcagtca ggtcctgttc
     2821 tccgggccga tcgagaactg cattcgagac ttggtggctg tgataggccc gcagggtgcc
     2881 ttggcttcgg gatggcgaga gaagttcctc gaacgtcaga ccataatcga acgtcagcaa
     2941 ccaaccgcgg cgcagacgcg tcgccgcatt tcgccaccag gtcaacgccg ccggacaggt
     3001 ttcgacaatg aacccgtccg gcaacaccgc caacagactc gcatccgatt gaaagaactc
     3061 gttcgggtct gacatccccg tagggctgct gtccgggcct gtcgattggg gccgatccag
     3121 ccgcacccag gcaaacgcat ccccgattct gccaacgccc cactcgcgcc agttcatggc
     3181 tgcggcgtcc caagcccatc gccggactgg catggcgtcc agcaactcgt tggcgaagat
     3241 gacgccctgg acgccacagt cgggcagact atcccagtca cttgcccaac gaacagaagc
     3301 ggcgcatggc ccgagtgtct ccttctgcca ggcgcgccgc gcgatcgacg gttccagaat
     3361 ccagtactcg atttggctgc gcccgccccc gcgccgcctg aaccagtcga caatatccct
     3421 ggccaaccgg ccgtcgtgcg ccccgctttc aacgatctgg agggggccgt cattgagtcc
     3481 cgccccccat tgcgcgaacg cgaaggccag caactctccg aaaaggccgc cggcgctcac
     3541 gcttgtgtag tagtccccat gccgtccgac cggggatcgc ctttcatagt agccgaactc
     3601 cggatggtac aacgccaggt ccatgaatcg cgcgaaactg atcggcccgg cagcttcgat
     3661 ctccgcgcga atcacatccg ccagccccga catttcgcga cactgcgtcc ggtctgatgt
     3721 ccggcctgcg gccaaaccgc gcccggatag ggaactgtga gaggcccgtc gctcctgaag
     3781 gccgtctctt gtggaaacag tgtgccgcat tcttgtcttc cctgggccgg gcacggctct
     3841 tttcttctcg ttcgttctgg cggacttcac gggcgcacgc tgaggtggtg tctgacaatt
     3901 ggccggtgtt tccttgtgcc caaggcggtc gggggcgaag caccagccaa ttgtcggaca
     3961 ccacgctagc cggaaggaca cgcgatcggc aaggcgcatc gcggacagca ggagctccgc
     4021 ccatgaacct cgcatctggt ccgcagcgcg cgtgtgcgtc aatctcgatt ggccgagagt
     4081 gcggtggtcc ccacggttat ttcatttaaa ttcctgaagg gttgctgttg ttgaggttag
     4141 aaataacgct tatcaattgg cgtggtttgg tgcgtggttg gcgtgtgatg atctcgacaa
     4201 accacgaact gttcagtgcc gatccgcccg tttcctttct gaaaaaacta caacctcaac
     4261 tgcaacctct cggccctgcg catctgcctg atcgcg
//
LOCUS       DAOQRO010000302         4295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22024_length_4295_cov_4.091509, whole genome shotgun sequence.
ACCESSION   DAOQRO010000302 DAOQRO010000000
VERSION     DAOQRO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22024_length_4295_cov_4.091509"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKK20_10750"
     CDS             <1..262
                     /locus_tag="PKK20_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNV00407.1"
                     /translation="LVEGEQRVSIHGEKIAVKGKVHTLGGFSAHAGQTDLLNWFAALA
                     PSRPQVVLTHGEDEPRRALARKIQQAHGIEASLPKLEQTMTI"
     gene            272..2779
                     /locus_tag="PKK20_10755"
     CDS             272..2779
                     /locus_tag="PKK20_10755"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV00408.1"
                     /translation="MTSNNTTSPGRPAARTSSTLSRYECGPFTFSDKDYYDRHLMFDH
                     AVDIPEASPRQRYEAVACSVRDLLTQRWIRTQQTHDRRNAKRVYYVSMEFLIGRALAS
                     NILNLGVERWVAEDLQSDQRDWRAVFDEEPDAGLGNGGLGRLAACFIESLATLEIPAF
                     GYGLRYEYGIFRQGLANGWQNEQPDNWLRYPDPWEVVRPQESVEVKLNCSFHTRSGAL
                     KVLPGCPSTLIGIPYDRPVVGYGGKTINTLRLWAAGTPDYFDFQEFSSGDFVGALAET
                     LTAETLTRVLYPDDSTTAGQGLRFLQEYFLVACSLADMIRRFRKSNTDWADLPNKAAV
                     QLNDTHPALAVPELLRLLLDEAKLGWDDAWQITQATLAYTNHTLLPEALEKWPVRWLE
                     ALLPRHLELIYEINRRFLDKVRSHHAGDDARVRRVSLIEEGAERKVRMAHLAIVGTHS
                     TNGVSAIHTRLLKSRVVPDFAALFPDRFNNKTNGVTPRRWLLQANPPLARVITDAIGD
                     AWITDLAQLERLRPLADDAGFRAAFRQAKGAAKESFAAWTKNALGQAIDTETLFDSQI
                     KRIHEYKRQLLNALHIVVLYNRLRDNPHCDAPPRTFLFAGKAAPAYRLAKLTIKFIND
                     LAATIDRDPAVRGRLKVVFLPDYCVTLAERLIPASEVSEQISTAGYEASGTSNMKFMM
                     NGALTIGTRDGATIEMAEAAGEENLFLFGLTAEQVALSRGWYSPHWHYQNEPETRDAL
                     DRIFSGHFGSADPGVIEGLRDALLNHGDYYLHLADLKSYGEAHERIGQLYADPEAWAR
                     KAVLNIAASGRFSSDRAISEYAHEIWNIPPCPIPDEA"
     gene            2889..4034
                     /locus_tag="PKK20_10760"
     CDS             2889..4034
                     /locus_tag="PKK20_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00409.1"
                     /translation="MIATRHITLWLANLALSLVTLAAAGQDPTAPAPPDAARPLTQAY
                     RVRPLDYAMMAEELPHRVSIEVKLVELRGADAQAVMIGRLLDAQPAPADTNISAAGIH
                     RSAALGNLRNDARFSAIFAKLDEPQTNRTAELQGDQLDWPGRHLTNAANMRLSAELGP
                     AASAVLTDPQCRLLVRALGQNPHASVFSTPRITVASGQQTRVQMVNLISVRTGIHPDA
                     VVQPMTWVRTNTPVYTLAQVPVGPSLDLAPIVRADEQGIDLIVTATLTEFLGYDPPIR
                     SRRVQVWKNGAKKRIDVPLPRFRVRQMQAPVRIEDGQTVLVATWPGPDIDPTQRPNSR
                     SAGLSGRSQPRPAFQRFLVFLTPRLAHPVAPSPSATRRLEAAPSASP"
BASE COUNT          821 a         1548 c         1222 g          704 t
ORIGIN      
        1 cctggtcgaa ggagaacagc gggtcagcat ccatggcgag aagatcgccg taaaaggcaa
       61 ggtgcacacc ctcggcggat tcagcgcgca cgccggccag accgacctgc tcaactggtt
      121 cgcggcgctc gctcccagcc gaccgcaggt ggtcctgacc cacggtgaag acgagccgcg
      181 ccgggccctg gccaggaaga tccagcaggc gcatgggatc gaagcgtctc tcccgaaact
      241 cgaacagacc atgacaatct gaaggaaacc gatgacttcg aacaacacca cgagcccggg
      301 ccgcccggct gcccggacct cttccactct gagccgctac gaatgcggcc cgttcacctt
      361 ttcggacaag gattactacg accgccacct gatgttcgat catgcggtcg acatcccgga
      421 agcgtcgccg cggcagcgtt acgaagcggt ggcctgctcg gtgcgcgacc tgctgaccca
      481 gcgctggatt cggacgcagc agacccacga tcgtcgcaac gcaaaacgtg tgtattacgt
      541 ctcgatggag ttcctcatcg ggcgtgccct ggcctcgaac atcctcaacc tcggcgtcga
      601 acgctgggta gccgaggatc tccagtccga ccagcgtgac tggcgcgccg tgttcgacga
      661 ggaacccgat gccggcctgg gcaacggcgg tcttggacgt ctggcggcct gctttattga
      721 atcgctagcc acactcgaaa tccccgcctt cggatacggc ctgcgttacg agtacggcat
      781 cttcagacaa gggctggcca acggctggca gaacgaacag cctgacaact ggctgcgtta
      841 ccccgatccc tgggaagtcg tgcggcccca ggaatcggtc gaggtcaagc tcaactgctc
      901 gttccacaca cgcagcggcg ccctcaaggt ccttcccgga tgcccctcga cgctcatcgg
      961 catcccctat gaccgcccgg tcgtcggcta cggtggcaag acaatcaata cactgcgcct
     1021 ctgggcggcc ggcacgccgg attacttcga ttttcaggag ttcagcagcg gcgatttcgt
     1081 gggcgccctg gccgagaccc tcacggccga gactctcacg cgggttctct atcccgacga
     1141 ttcgaccacc gcaggccagg gtctccggtt tctgcaggag tacttccttg tcgcctgctc
     1201 tctggctgat atgatccgcc gcttccgcaa aagcaacacc gactgggcgg acctgcccaa
     1261 caaggcggcc gtccagctca acgacaccca ccccgctctg gctgtgcccg aactgttgcg
     1321 gctcctgctc gacgaagcca aactgggctg ggacgacgcc tggcagatca cccaggccac
     1381 gctggcctac accaaccaca ccctcctgcc tgaagccctc gaaaaatggc cggtccgatg
     1441 gctcgaagcg ctcctccccc gccacctcga actcatctac gaaatcaacc gccgattcct
     1501 tgacaaggtc cgctcacacc atgccggcga cgacgcccgt gtccggcgcg tcagcctgat
     1561 cgaggaaggc gccgagcgca aagtgcgcat ggcgcacctg gccatcgtcg gcacccacag
     1621 caccaacggc gtttcggcaa tccatacccg gttgctcaag agccgcgtgg ttcccgattt
     1681 cgcggccctg ttccccgacc gattcaacaa caagaccaac ggagtcacgc cccgacgctg
     1741 gctgcttcag gccaacccgc cgctggcccg cgtgatcaca gacgccatcg gcgacgcctg
     1801 gatcaccgac ctcgcacaac tcgagaggct gcgcccgctt gccgacgatg ccggttttcg
     1861 cgcggccttt cggcaggcca agggagccgc caaggagagc ttcgccgcgt ggaccaagaa
     1921 cgccctgggg caagcgatcg acaccgagac gctcttcgac tcccagatca agcgcatcca
     1981 cgagtataaa cgccaactcc tcaacgccct ccatatcgtg gtgctgtaca accggctgcg
     2041 cgacaacccg cactgtgacg cgccgccacg cacgttcctg ttcgccggca aagcggcgcc
     2101 cgcatacagg ctggccaagc tgaccatcaa gttcatcaac gacctcgccg caaccatcga
     2161 ccgcgacccc gcggtccgcg ggcgcttgaa ggtcgtgttt ctgcccgact attgcgtcac
     2221 cctggccgaa cggctgatcc cggccagcga agtgtcggag caaatctcga ccgccggcta
     2281 cgaggccagc ggcaccagca atatgaagtt catgatgaac ggcgccctca ccatcggcac
     2341 gcgcgatggc gccaccatcg agatggccga ggcggccggc gaggagaacc tgttcctttt
     2401 cggcctcacc gccgagcagg tggccctaag ccgcggctgg tacagtccgc actggcatta
     2461 ccagaacgaa cccgaaaccc gcgacgccct cgaccgaatc ttctccggcc acttcggttc
     2521 cgccgatccc ggagtgatcg agggtctccg cgacgccctt ttaaaccacg gcgattacta
     2581 ccttcacctg gccgatctca aatcgtacgg cgaggcgcat gaacgcatcg gacagctcta
     2641 cgccgacccg gaggcctggg cgcgcaaggc cgtcctcaac atcgccgcct ccggacgctt
     2701 ctcgagtgac cgggcgatct ccgagtacgc ccacgaaatc tggaacatcc cgccctgccc
     2761 catccccgac gaagcctgag ccgcggcgag ggctggcccg attattttac acgcttcgcg
     2821 aggaaccata agcccgccaa gctcggccga cccgagcgga tgggcgacga tctgcttgga
     2881 ttttgtctat gatagcgaca cgccacatca ccctctggct cgccaacctg gccctgagcc
     2941 tcgtgactct ggcggccgct gggcaagacc cgaccgcccc ggcgccccct gacgccgcac
     3001 gcccgctcac gcaagcatac cgcgtccgcc cgctcgatta cgcgatgatg gccgaggagt
     3061 tgcctcatcg agtgtcgatc gaggtcaaac tggtcgagct aaggggcgcc gacgcgcagg
     3121 ctgtcatgat cggacgcctc ctggacgccc agccggcgcc cgcggacaca aacatatccg
     3181 cggccggcat tcatcgctca gcggcgctcg gcaacctgcg gaatgatgcc cgcttctccg
     3241 caatctttgc caaactggat gagccccaga ccaaccggac cgccgagttg cagggagacc
     3301 aactcgattg gccaggccgg cacctcacca acgccgctaa catgcgcctc tcagccgaac
     3361 tgggtcccgc cgcgtcagcc gtcctgaccg acccccagtg tcgcctcctg gtccgggccc
     3421 tgggccagaa tccccatgcc agcgtcttct ccaccccgcg catcacggtt gccagcggcc
     3481 aacagacgcg ggttcagatg gtgaacctga tcagtgtgcg cactggcatc catcccgatg
     3541 ccgtcgtgca gccgatgacc tgggtccgca caaacacccc cgtctacacg ctggcacagg
     3601 tgccggtcgg tccgagcttg gacctggccc cgatcgtgcg cgccgatgag caggggatcg
     3661 acctgatcgt caccgccact ctgacggagt tcctcggata cgatccgccg attcgaagtc
     3721 gcagggtgca ggtctggaag aacggcgcaa aaaaacggat cgatgtcccg ctcccgcgat
     3781 tccgcgtgcg ccagatgcag gcgccagtca gaatcgaaga tgggcagact gtgctggttg
     3841 cgacctggcc cggcccggac atcgatccga cgcaaaggcc gaactcgcga tcggcgggtc
     3901 tctccggccg aagtcagcca cgacccgctt tccaacgctt tctggtcttc ctgaccccca
     3961 gactcgccca ccccgtcgcc ccctcgccct ccgccacacg ccgactcgaa gctgcgcctt
     4021 cagcctcccc gtgacacgcg tacgcgaaag gaaaggccta tggcaaagca gacctcttca
     4081 tcgaaccctc cgcggcccat atcaatagct tcagccagtt tactctgccg cgtctcgcca
     4141 cgttcacata gaataggcat tctcagcttg tgggccgcgt gcccctgacc cggcgcctgc
     4201 accgatgtct gtcctcgcgc cgcgtggggg cacgcggcct tggagtttgg acattctgaa
     4261 gacggatttt ggcggggggc agtgtgaggc ggcga
//
LOCUS       DAOQRO010000303         4291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22058_length_4291_cov_3.996695, whole genome shotgun sequence.
ACCESSION   DAOQRO010000303 DAOQRO010000000
VERSION     DAOQRO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22058_length_4291_cov_3.996695"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKK20_10765"
     CDS             complement(<1..241)
                     /locus_tag="PKK20_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV00410.1"
                     /translation="MKNTLETRVGFLFALAMVAGMILLEMVGGLDFLRGGLRLNARFK
                     NIQELKKGDPVKMAGKPVGRVEDIQFADSMVLVQMV"
     gene            complement(238..1005)
                     /locus_tag="PKK20_10770"
     CDS             complement(238..1005)
                     /locus_tag="PKK20_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV00411.1"
                     /translation="MNATAQQGVNLRARDVCKSYDGQRVLNGIDFHVERGEVFVLMGP
                     SGSGKTVLLKHLIGLEVPDRGEILIEGQPIHSPGVVDRYRLAMVFQSGALLNSLSVAD
                     NVGLYLSEHRLHPPETIARIVTEKLALVGLKGIENKLPSELSGGMKKRVSIARALVIE
                     PQLILYDEPTSELDPLIAVTIGAEILRLNKRIHVTSIVVTHDRELAFGIADRIALMNE
                     GELIALGPPDQVKANPHPLVQRFLQADFRFNPESITP"
     gene            complement(1005..1748)
                     /locus_tag="PKK20_10775"
     CDS             complement(1005..1748)
                     /locus_tag="PKK20_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV00412.1"
                     /translation="MFQNVGEILMLLWRTAWALPMTWRQRRKVFDQLFEIGNASLLMA
                     CLLSLFIGGVLALQTGPVLAERGLTSFIGQIVGLSMCRELAPAMMAMLIAGRIGSSMA
                     AELGSMTVYQEIDALRSMNINPIQYLVLPRLVAISLALPVLVVFSILIGWLGGAVVSV
                     YNYQIAISYEGYFNSLRDGIDLIDVVNGLFKSAAFAVVIGLVSCHQGLHTIGGPRGIG
                     RSVTKAVANSIILILILDYFLTRLLMHLD"
     gene            complement(1729..3261)
                     /locus_tag="PKK20_10780"
     CDS             complement(1729..3261)
                     /locus_tag="PKK20_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00413.1"
                     /translation="MRWVVLALLSAGCGVGVPLCGGAQAQDAAWGPFYHRFRLTLEPG
                     ERTEALGPFYYRQDVWEEWPEELGVLDLPGEPTPVQDAATTLALPPLFSSLRCPGVPA
                     LSWDFLYPIMTYDRYGAESRLQVAQLLSFGAGRSQDGTASRGFSLFPLFFFRRSDDPA
                     RDYSAVFPFYGHTQQRLFRDEWRVVLWPLYAQSRKKDVVTDNYLVPLFHRRHGDGLRG
                     WQFWPLIGQERKETTWRTNSLGGVETLWGHEALFVLWPMFFHNTVDIGSTNAMRQRVL
                     LPFYSLQLSPAKETRTYLWPLGPTFVDAHAEGYRQVGLPWPLIVFARGEGKTTDRVFP
                     LYSHSRYREGETTWYLWPILWQRHIRTQALERDLTRVGFFLYTDIAERQKATGRTRRR
                     TDVWPLFASRRDFDGNERFQVFAPIEAILSSNPSVTRNWSPLWALWRSEKNARTDARS
                     RSLLWNLYRSEAAPGVKKCSILFGMIRYEAGPTGARWRWLGLFGGKPGRGATRDHVPE
                     RR"
     gene            complement(3320..>4291)
                     /locus_tag="PKK20_10785"
     CDS             complement(3320..>4291)
                     /locus_tag="PKK20_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNV00414.1"
                     /translation="KPDIIVNNRSGAPGDYDTPEQRIGAFQIARPWETCMTICQQWAW
                     KPNDAMKSLKECLQTLVLCAGGDGNLLFNVGPMPDGRIEPRQVERLREMGAWLARHGQ
                     TIYGTRGGPWKPSKALASTRRGREVFVHILRWDSDTITVPTLPMKIIDSTLLTGGRVR
                     VTQTSEGVSFAVAPEDRSDIDTIVRLELEGPAMDIAPMSLQSAIQASASTVYQNMAEY
                     GAESAFDDDDHTRWATDAGTKTAWVARDFGRPLTFHRVRIREAYPGRVERFELQYKTG
                     SDWKTIFAGTQLGENFTRSFEPVSAREVRLNILEANEGLTIWEVRLD"
BASE COUNT          858 a         1415 c         1272 g          746 t
ORIGIN      
        1 aaaccatctg caccaacacc atcgaatcgg cgaactgaat gtcctcgacc cggccgaccg
       61 gcttgccggc catcttgacc gggtcgccct tcttgagttc ctggatgttc ttgaagcggg
      121 cgttcaaacg caggccgccc cggaggaaat cgaggccgcc caccatctcg aggagaatca
      181 tgcccgccac catggcgagc gcgaaaagaa agcccacgcg cgtttcgagt gtgttcttca
      241 tggggtgatt gattcggggt tgaagcggaa atccgcctgc agaaagcgct gaaccagcgg
      301 atgaggattc gccttgacct ggtccggcgg gccgagggcg atcagctcgc cctcgttcat
      361 gagcgcgatc cggtccgcaa tgccgaacgc cagctcgcgg tcatgcgtca ccacgatcga
      421 ggtgacgtga atgcgcttgt tgagtcgcag gatttcagcg ccgatcgtca cggcgatgag
      481 cggatccagt tcgctggtcg gctcgtcgta aaggataagt tggggctcga tcacaagggc
      541 ccgcgcgatc gagacccgct tcttcatgcc gcccgacagc tcgctaggca gcttgttctc
      601 gattcccttg agcccgacca gggccagttt ttcggtgaca atccgcgcga tggtctcggg
      661 cggatgcagt cggtgctcgc tcaggtacag gccaacgttg tcggccactg agagggaatt
      721 gagcaatgcg cccgactgga acaccatggc cagtcgatac cgatccacca cgccaggcga
      781 atggatcggt tgcccttcaa tcagaatctc accgcggtcc gggacctcga gcccgatgag
      841 gtgcttgagc agcacggtct tgccgctgcc gctcggcccc atcaagacga acacctcgcc
      901 gcgctcgaca tggaagtcga tgccattgag cacgcgctga ccgtcgtaac tcttgcacac
      961 gtcgcgtgcc cggaggttca ccccctgttg tgccgtggcg ttcatcagtc caggtgcatc
     1021 agcagtcgcg tcagaaagta atccaagatc agaatcagga tgatcgagtt ggcgacggcc
     1081 ttggtcacgg agcgcccgat gccccgcggc ccgcctatgg tgtgcaggcc ctgatggcac
     1141 gagaccagac cgatcacgac ggcaaatgcc gcgcttttga acaggccgtt cacaacgtcg
     1201 atcaggtcaa tcccgtccct gaggctgttg aaatagccct cgtaggaaat cgcaatctgg
     1261 taattgtaaa ccgacaccac ggcccccccc agccacccga tcaggatcga gaacacaacc
     1321 aacaccggca gcgcaagact gatcgccacc aaccgcggca acaccaggta ttggatcgga
     1381 ttgatgttca tcgagcgcaa cgcgtcgatc tcctggtaca cggtcatcga gcccagctca
     1441 gccgccatcg atgatccgat gcgtccggcg atcagcatgg ccatcatggc gggagccagt
     1501 tcccggcaca tcgaaagccc gacgatttgg ccgatgaagc tggtcaggcc gcgctcggcc
     1561 aacaccggac cggtctggag ggcgagcacc cccccgatga agagcgagag caggcaggcc
     1621 atcaataggc tcgcgttccc aatctcgaac aactggtcga acaccttgcg ccgctggcgc
     1681 cacgtcatgg gcaaagccca cgccgtgcgc cacagcagca tcagtatttc accgacgttc
     1741 tggaacatgg tctctcgtcg ccccccgccc cggcttgccg ccaaacaggc ccagccagcg
     1801 ccaccgggca cctgtaggcc cggcttcata ccgaatcatg ccaaagagga tcgagcactt
     1861 tttaacgcct ggagccgctt ccgaacgata gaggttccag agcaaggaac ggctgcgcgc
     1921 atcggtccgc gcgttcttct ccgagcgcca caacgcccag agaggcgacc agttccgcgt
     1981 gacgctcggg ttcgaggaca ggatcgcctc gattggcgcg aagacctgga atcgttcgtt
     2041 gccatcgaaa tcgcgccggg acgcaaacaa cggccaaacg tccgtccgcc gcctggtccg
     2101 gccggtcgcc ttctgacgct cggcaatgtc cgtgtaaagg aagaaaccga cgcgtgtcag
     2161 atcgcgctcg agcgcctgcg tgcggatgtg cctctgccaa agaatcggcc agaggtacca
     2221 ggtggtctct ccctcccgat accggctgtg ggaatagagc ggaaaaaccc gatcggtcgt
     2281 cttgccttcg ccgcgcgcga acacgatcag cggccaaggg agcccgactt gccgatagcc
     2341 ctcggcatgg gcgtcgacaa atgtcggacc caacggccat agataggtgc gcgtctcctt
     2401 ggcgggcgac aactgcaagc tgtagaacgg cagcaagacc cgctggcgca tcgcgttcgt
     2461 cgagccgatg tcgacggtat tgtggaagaa catcggccac agcacgaaca gtgcctcgtg
     2521 cccccagagc gtctcgacac cgcccaggct gttcgtgcgc cacgtggtct ccttgcgctc
     2581 ctgtccgatc aaaggccaga actgccagcc tcgcagcccg tcgccgtgtc gccgatgaaa
     2641 caggggcacc aggtaattgt cggtcaccac gtccttcttc cggctttggg cgtacagcgg
     2701 ccaaagcact actcgccact cgtcccgaaa caaacgctgt tgggtatggc cgtagaacgg
     2761 aaacaccgcg ctgtagtctc gagccggatc atcggatcgc cggaaaaaaa acaacgggaa
     2821 caggctgaat ccgcgtgatg ccgtcccatc ttgcgaccgg ccagccccaa aactcagcaa
     2881 ctgcgccact tgaagccgcg attccgcccc gtagcgatca taggtcatga tcgggtagag
     2941 aaagtcccac gacaacgccg ggacgccggg acaccggaga ctcgagaaaa gcggcggcaa
     3001 agccagggtc gtggcggcgt cctggacggg tgtcggttcg ccgggaagat cgaggacgcc
     3061 gagttcttca ggccattcct cccaaacgtc ctggcggtaa taaaaaggtc ccagcgcttc
     3121 cgtgcgctcg cctggttcga gcgtaagccg aaagcgatga taaaacggcc cccacgccgc
     3181 gtcctgcgct tgcgccccgc cgcagagagg gacgccgacg ccgcatccgg ccgacagcag
     3241 cgccaacacc acccatctca tgggaacaag gcaacgcact gtgctccagc ttgagagccg
     3301 acaccccgca ccgtcaagtc tagtcgagcc ggacctccca gatggtcagg ccttcgttgg
     3361 cctcgaggat gttcaaccgc acctcgcgcg cgctcaccgg ttcaaaggac cgggtgaagt
     3421 tctcccccaa ttgcgtccct gcgaagatgg tcttccagtc gctgcccgtc ttgtattgca
     3481 gctcgaaccg ctcgacgcgc ccgggatagg cctcgcggat gcgcacccga tgaaaggtca
     3541 ggggccgtcc gaaatcgcgg gcgacccacg cggtcttcgt cccggcatcg gtcgcccacc
     3601 gcgtatgatc gtcgtcatcg aaagcgctct cggcgccata ttccgccatg ttctgataaa
     3661 ccgtcgaggc gctggcctgg attgccgact gcaggctcat cggagcgatg tccatcgccg
     3721 gcccctccaa ttcaaggcgg acaatcgtgt cgatgtcgga ccggtcctca ggcgcaacgg
     3781 cgaacgagac accttcggac gtctgcgtca cgcggacgcg gcctcctgtc aggagcgtgc
     3841 tgtcgatgat cttcatcggc agagtgggca cggtgatcgt gtccgaatcc cagcgcagga
     3901 tgtgaacgaa cacctcgcgc ccccggcgtg tgctggcgag cgccttcgag ggtttccacg
     3961 gaccgccgcg cgttccgtag atggtctggc cgtgccgggc cagccacgcc cccatctcgc
     4021 gaagccgctc gacctgacgc ggctcgatcc gtccgtcggg catcggcccc acattgaaca
     4081 acaggttgcc atccccgccc gcgcacaaca cgagtgtctg aaggcattcc ttgagcgatt
     4141 tcatggcgtc gttcggcttc caagcccact gctggcaaat ggtcatgcac gtctcccacg
     4201 gccgagcgat ctggaatgcg ccgatccgct gctcgggtgt gtcgtaatcg ccgggcgcgc
     4261 cgcttcggtt gttgacgatg atgtccggct t
//
LOCUS       DAOQRO010000304         4277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22159_length_4277_cov_4.114401, whole genome shotgun sequence.
ACCESSION   DAOQRO010000304 DAOQRO010000000
VERSION     DAOQRO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22159_length_4277_cov_4.114401"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1506
                     /locus_tag="PKK20_10790"
     CDS             280..1506
                     /locus_tag="PKK20_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HNV00415.1"
                     /translation="MRFPFALSAKIAGHIIKHKLRGTPKFAMVLQLEPLHACNLSCTG
                     CGRIREYSTSLKDSVPLDTCLAAAAECGAPMVSICGGEPLIYPQVEALVEGLLAQGRI
                     VYICTNGVLMRRKLRDYLAHTHSPAREGLLRRLLAEELITASQAETIRRGRTGEGPVI
                     APSRWLYWNVHIDGLESTHDRIVEREGVFRECVAAIRLAKELGFQVATNTTVYRETSV
                     REIEEMFAFFSWMGVDGHTISPGYDYDAAKRDMAQRLGKRPEDFFLTRAMTREKFAGI
                     LDWGRRFTIFGTPVYQEFLAGRRELTCSAWAIPTYNIKGWKAPCYLITDDHYAGYGQM
                     LERVDWDRYGVVDGVFRDPRCENCMVHCGYDPSGALGTDYRRGDHWKNFIYNFGPRPP
                     PAIEGQRVNAFNRTAS"
     gene            1503..2960
                     /gene="hpnJ"
                     /locus_tag="PKK20_10795"
     CDS             1503..2960
                     /gene="hpnJ"
                     /locus_tag="PKK20_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HNV00416.1"
                     /translation="MSRVLFLSPPSFDGFDGGAGSRYQARREVTSYWYPTWLAQPAAL
                     VPGAKLMDCPPHEIGIEECLRHAHGYDHVIIHTSTPSLRNDCRVAEAIKARRPDTTIG
                     FVGAHAAVLPTETLKASPAIDWVGRKEFDYTCKEVAEDRPLADIAGLSYRKEGRIIHN
                     RERDLIADMDALPWVVDVYKRDLVIEKYFIGYLLHPYLSLYTGRGCPGQCTFCLWPQT
                     IGGHRYRVRSAGNVADEMAYAKRLFPEVKEFFFDDDTFTANLPRARDVARKLKPLGLV
                     WSCNSRANLNFDTIKLMKDCGLRLLLVGYESGSDEILRRAKKGVTVDQMRAFTKACHD
                     IGVIIHGTFILGLPIETRETIEETIRFAQELDVFSIQVSLAAPYPGTELYEMARQNGW
                     FAREDKTDILHQDGLQHSALEYPGLSKEEIFEAVEVFYRRYFLRPKPILRILKTMLED
                     KEVCVRRLREGYEFFRTMAQRREIQGGACEPATTR"
     gene            3077..>4277
                     /locus_tag="PKK20_10800"
     CDS             3077..>4277
                     /locus_tag="PKK20_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00417.1"
                     /translation="MIKRNIQLSDFEQRASPGDAAFEVFEGELRRFIQDSVPFGTGLE
                     RDDSDEQEARFQRLALALFRLQHRAVGPLFRLCQRCGIAPEDIGDWRAIPALTTTAFK
                     DYAVTSLHLEARTRVFHSSGTTGHRPSRHFHSAGSLAVYEASLLPFFRARLMGGVGQG
                     KGEFTFLALTPDPAEAPHSSLAHMFATVIEAWGADDSAFVGAVECDGGWRVDTAASTA
                     LLRRCARNGRPVCVLGTAFGFVHLLDAMHQDCLQCALPPGSRALETGGYKGLSRSVLK
                     PDLHRLIGTTLGIAPTHIVSEYGMSELSSQAYDACVPASPLARGAVDPPARGATNSGA
                     APGIETGAPQWGEEDRRLSAGHSAGEPAPAGARVFHFPPWARSVVGSPENGIEGRDGR
                     GGAVGRRA"
BASE COUNT          756 a         1322 c         1387 g          812 t
ORIGIN      
        1 gaactggctg ctggagttcg tatttgccat cgagacagtc gggtctatag tgcccgagtg
       61 aaatttagct gcaaccagat gctctttgca actttggtgg caatcgcatt gctcgccagc
      121 ggttgcggcg gtttggcgct ctctccctca atttccccgc tcatgtttct gattcccggt
      181 ctcggacagg cccagccgca atcggctcca ggcagcccgg tgtcgacccc ctccgcgccg
      241 cctgtgcccg cactggccca ggcttcgtag cgccagcgta tgcgctttcc gttcgcgttg
      301 tcggcgaaga tcgccgggca cattatcaag cacaagcttc ggggcacccc gaagttcgct
      361 atggtcttgc agctcgaacc gctgcacgcc tgcaacctgt cctgcaccgg gtgcggacgg
      421 attcgggagt actcgacgag tctcaaggac agcgtgccgc ttgacacgtg tctggcggcg
      481 gccgcggagt gcggggcgcc gatggtgagc atctgcgggg gcgaaccgct catctatccg
      541 caggttgaag cactcgtcga ggggttattg gcgcagggcc gcatcgtgta catttgcacc
      601 aatggcgtgc ttatgcgccg gaagctccgc gactacctgg cacacacgca cagcccggcc
      661 cgggaaggct tgctgcgcag gcttctggcc gaggagttga tcaccgccag ccaggccgag
      721 acgatccggc gcggcaggac cggagagggg cctgtcattg cccccagtcg atggctctac
      781 tggaacgtcc acatcgatgg tctcgaatcg acgcatgacc gcatcgtcga gcgcgagggg
      841 gttttccggg agtgcgtggc cgccatccgc ctggcgaaag agttgggctt tcaagtggcc
      901 accaacacga ccgtctaccg cgagaccagt gtgcgcgaaa tcgaggagat gttcgccttc
      961 ttctcctgga tgggtgttga tggtcacacc atttcgcccg gatacgatta cgacgccgcc
     1021 aagcgcgaca tggcgcagcg actggggaaa cggcccgagg acttctttct tacccgggcg
     1081 atgacgcggg aaaagtttgc cgggattctc gattggggca ggcggttcac gatcttcggc
     1141 acgcccgttt accaggagtt tctggcgggc cggcgcgaat tgacatgcag cgcctgggcc
     1201 attccgacat acaacatcaa gggctggaaa gccccctgct atctcatcac cgacgatcat
     1261 tatgccgggt acggccagat gctcgaacgg gtggactggg acaggtatgg cgtggtcgac
     1321 ggcgtgtttc gggacccgcg ttgcgagaat tgcatggtcc actgcggcta cgatccgagc
     1381 ggcgcgctgg gcaccgacta ccgccgcggc gatcactgga agaactttat ctacaacttt
     1441 gggccgcgcc cgccgcccgc catcgagggg caacgggtga acgccttcaa tcggaccgca
     1501 tcatgagccg agttctattt ctctctcctc cgagcttcga tggcttcgat ggaggcgccg
     1561 gatcgcgcta ccaggccaga cgcgaggtga ccagctactg gtatccgacg tggctggccc
     1621 agccggcggc tttggtgccc ggggcgaagc tgatggactg tccgccgcat gagatcggga
     1681 tcgaggaatg tctccgacac gcgcacggct acgaccacgt tatcatccac acctcgacgc
     1741 cctctctgcg caacgactgc agggtggccg aggccatcaa ggcccggcgc ccggatacga
     1801 cgatcggatt tgtcggagcc cacgcggccg ttctgcccac cgagacgctc aaggcctcgc
     1861 cggccatcga ttgggtgggg cgcaaggagt tcgattacac atgcaaggaa gtggccgagg
     1921 accgccccct ggcggacatt gccggcctgt cctaccgcaa agagggccgg atcatccaca
     1981 acagggaacg ggatctgatc gccgacatgg acgctctgcc ttgggtggtc gacgtgtaca
     2041 agcgggacct tgtcatcgag aagtacttca tcggctatct gcttcatccc tatttgagtt
     2101 tatacaccgg acgcggctgt ccggggcagt gcacgttctg cctttggccg cagaccattg
     2161 gcggacacag gtatcgcgtc cgctcggcgg ggaatgtcgc ggacgagatg gcgtatgcga
     2221 agcgactctt tccggaagtg aaggagttct ttttcgacga cgacaccttt acggcgaatc
     2281 tgccgcgcgc gcgcgatgtg gcccgcaaac tcaagccact gggcctggtc tggtcgtgca
     2341 acagccgcgc caatctcaac ttcgacacga tcaagctgat gaaggactgc ggcctgcggc
     2401 tgttgctggt cggctacgag tcgggcagcg atgagattct gcgtcgggcg aagaaggggg
     2461 tcacggtcga ccaaatgcgc gcgtttacga aagcctgtca cgacatcggc gtcatcatcc
     2521 acggcacgtt catcctcgga ctgcccattg agacacggga gaccatcgag gagaccatcc
     2581 ggttcgccca ggaactggac gtgttcagca tccaagtatc tctggccgcg ccgtatccgg
     2641 gcaccgaact ctacgaaatg gcccgccaga acggctggtt cgcccgagag gacaagaccg
     2701 acatcctgca tcaggacggt ctgcagcact cagccctcga ataccccggc ttgagcaagg
     2761 aggagatctt cgaggcggtc gaggtcttct atcgacgcta cttcctgcgg cccaaaccga
     2821 ttctgcgcat tctcaagacg atgctcgaag acaaggaagt ctgcgtgcgg cgtctgcgcg
     2881 agggttacga gttcttccgc accatggccc agcgccgtga aatccaaggc ggggcttgcg
     2941 aacctgcgac aacccgctaa catcgcccct ggctgtggcg gcgaaccagt caggcgcccc
     3001 tgttttcgat tgcacatcac ctcgagcgag ccgcggctca tcaaggtgat tcggaggggc
     3061 gcgcgaacaa aaccgcatga ttaagagaaa cattcaactg tcggatttcg aacaacgcgc
     3121 gtctccagga gacgcagcgt tcgaggtgtt cgagggggag ttgcgccggt ttattcagga
     3181 ttccgttccg ttcggaacag gtctggaacg ggatgattcg gacgagcagg aggcacgatt
     3241 ccagcgactt gccctggcgc tctttcgcct ccagcaccgc gcggtcggtc cgctgtttcg
     3301 actgtgtcaa cgatgcggga tcgcgccgga agacatcggg gattggcgcg cgattcccgc
     3361 gctgaccacg acggcattca aggactacgc ggtcaccagc ctgcatttgg aggctcggac
     3421 acgcgtcttt cattcgagcg ggacgacggg gcatcgcccg agccggcact ttcacagcgc
     3481 cggttcattg gcggtgtacg aggcgtcgct gctgcctttc tttcgggcgc gcctgatggg
     3541 tggggtgggg caggggaagg gcgaattcac attcctggcc ttgacgccag acccggccga
     3601 agcgccgcat tcgtccctgg cgcacatgtt tgccacggtc atcgaggcct ggggcgccga
     3661 tgactccgcc tttgtcggag cagttgaatg cgacggcggg tggcgggtcg atacggccgc
     3721 gtcgaccgcg ctgctgcgcc ggtgcgcgcg gaatggccga ccggtttgtg ttttgggcac
     3781 ggcatttggc tttgtgcatc tgctcgatgc gatgcatcag gactgcctgc agtgcgcgct
     3841 gccccccggc tcgcgcgcgc tcgagacggg gggttacaag gggctatcga ggagcgtctt
     3901 gaaaccggac ctgcaccggt tgattggcac cacgctcggt attgccccga cgcacatcgt
     3961 ctccgagtac ggcatgagcg aactcagctc gcaggcctac gacgcctgcg tgccggcgtc
     4021 cccgctcgcc cgaggcgctg tcgacccacc tgctcgcggc gccacgaact ccggcgccgc
     4081 tccgggcatc gagaccggcg ccccgcaatg gggtgaagag gatcgccgcc tgtccgccgg
     4141 gcattcggcc ggtgagcctg cgcctgcggg cgcgcgggtg tttcacttcc caccctgggc
     4201 gcggtcggtg gtcggttctc ccgagaacgg gatcgagggt cgggatggtc gcggcggtgc
     4261 cgtaggtcgg cgagcgg
//
LOCUS       DAOQRO010000305         4276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22164_length_4276_cov_4.655058, whole genome shotgun sequence.
ACCESSION   DAOQRO010000305 DAOQRO010000000
VERSION     DAOQRO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22164_length_4276_cov_4.655058"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1736)
                     /locus_tag="PKK20_10805"
     CDS             complement(33..1736)
                     /locus_tag="PKK20_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV00418.1"
                     /translation="MNKPSTPSTSDSPDIPVSSFYDTLHQLLSPEALSAPARPGTAGR
                     LDRYEVLQAIGQGGMGVVLLARDPTHRKPVALKVLRADLAKSLAARKRFLDEAQHMRR
                     LEHPGIVTVLDAGEEEGVPWFSMPYFEQGNLAKALAASGPMSDDRAVAITRSVAGALD
                     YAHRHGIIHRDLKPANILLSADGQAVVTDFGLARTVFNDSVVDPQRGHCEGTAPYMSP
                     AVARGEAEDTRCDIYSLGAVLYEMLTGQPPYKGPTSAAVLDQIKAGPPPPIRTLNAKA
                     NSRLAQVAETAMAADLRDRYARMADVVEDLDRVAAGREPLGARAIRRGLEERGLARGR
                     RLWFGVAAVLLAILGTWAWSAARPRIELVRSIDLGKGMASIEGWRTGDPPDFDADGVP
                     DFVVVKSKSFGLLSGDGAVLSHEVTVDEPHDSRTLSAGLPVDLNGDGYDEVSVSWAHE
                     STATLATVNMISHITARFTLPNTNSADRFGRPEWTRLCANALTDLEGDGRPELFATAN
                     SPRGMRARALVCYDVLSQTQLWSCAVAPNIDLVVPVDLNRDGCKEIVCGSYAPSAYLH
                     C"
     gene            complement(1932..2465)
                     /locus_tag="PKK20_10810"
     CDS             complement(1932..2465)
                     /locus_tag="PKK20_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00419.1"
                     /translation="MCSEKTYPSGEACETRFADLLEAGLESARPWRPEELAAAWDYHL
                     SAPIEFELGAMSAHRARALRDLAVAQGLTVSSVRALMQHPVPPIELLAMIARFAKACL
                     KSKDSPVPREVAGVLYYLAIAVAVTRLGTLVTRLRRGDLMRGLDWALARPWVDQSSKA
                     TLEQARASVTKATGGGV"
     gene            complement(2600..3361)
                     /locus_tag="PKK20_10815"
     CDS             complement(2600..3361)
                     /locus_tag="PKK20_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNV00420.1"
                     /translation="MQGHRARVGEEREGTFPSSHWSSISLATRPEEMGGDGALNRLLT
                     RYYAPLLAHLEYKFTLSREAAEDVLQDFMARKVLEGRLLGRADRSRGRFRTFLLNVLD
                     NFAKDGHRAQMRSGRRPSGGLVSLDDNLQEAERTVSRRDGDPFDRLWALTVIEEAEND
                     TRRFYEVKKRLDTWDAFYEGWFVPTRDGVERPTDEELARRHGFATARQASNAIVTAKR
                     GFGRMLRRAIARYAESESEVDDELLRLMSVFSRER"
     gene            3510..>4276
                     /locus_tag="PKK20_10820"
     CDS             3510..>4276
                     /locus_tag="PKK20_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HNV00421.1"
                     /translation="MATAQDAYEPDNTADNAKVITNGQTQNRSIHQAGNVDWAKFTIG
                     ASGATNVRIETSGASGDTEMWLYDPTRSPMQLAYDDDNGNGRFSLITLASLAAGTYYI
                     KITEYGNNGTIAAYTLRASWTEGGGGQPDLTKESDNISRTLGAPGDTFTITQRVRNGG
                     TAASGATKVYYYVNKDSRSTAAAAKVGERDLGALGAGSVSGEITFPWTAPANLPAGNY
                     FIGYWIDGQQSVAESNENNNIWWWDNITIQGANWQSEA"
BASE COUNT          817 a         1411 c         1282 g          766 t
ORIGIN      
        1 cttgccatgc accccaacat acaatatatc gtttaacagt gcaagtaagc actaggagcg
       61 tagctgccac acacaatctc cttgcacccg tcccggttca ggtccacagg gacgaccagg
      121 tcgatattcg gcgccaccgc gcaggaccag agttgcgtct gacttaaaac atcgtagcaa
      181 acaagtgcgc gcgcccgcat gcctctgggg gagttggccg tcgcaaacaa ctcggggcgc
      241 ccgtctccct cgaggtcggt cagggcgttc gcgcacaggc gggtccattc cggccttccg
      301 aagcgatcag cgctgtttgt gttgggaagc gtgaatcgcg cggtaatgtg gctgatcatg
      361 ttcaccgttg cgagcgtcgc cgtgctctca tgggcccacg aaacagagac ctcgtcatag
      421 ccgtctccgt tgagatccac aggtagaccc gccgatagag tccgactgtc gtgcggttcg
      481 tccaccgtca cttcgtgtga aagaacagca ccgtctcccg acaagagccc gaaggacttc
      541 gacttgacaa cgacaaagtc cggcacgcca tcggcatcga agtcgggcgg atctccggtt
      601 cgccatccct cgatactggc catccccttc cctaagtcga tcgagcgcac cagttcgatc
      661 cgcggacgtg ccgccgacca tgcccatgtc ccgaggatgg ccaataacac tgccgccacc
      721 ccgaaccaca ggcgtctgcc gcgcgccaag ccccgctctt caagacctcg ccggatggcc
      781 cgcgcgccca gcggttcgcg cccggcagcg actcgatcca ggtcctccac gacatcggcc
      841 atgcgcgcgt agcggtcccg caggtccgcg gccatcgctg tctcggcaac ctgcgccagt
      901 ctcgagttgg ccttggcatt cagggtcctg atggggggcg gcgggccggc tttgatctgg
      961 tccagtacag cggcggaggt cggccccttg tatggaggct ggccggtgag catctcgtag
     1021 aggaccgcgc ccagcgaata aatgtcgcaa cgcgtgtctt cggcttcacc gcgggcgacc
     1081 gccggggaca tgtacggcgc ggtgccctcg cagtggccgc gttgcggatc gacgacggag
     1141 tcgttaaaga cggtccgcgc caggccgaag tccgtcacga cggcctggcc gtcggcggag
     1201 agcaggatgt tcgccggctt caggtcgcgg tgaatgatcc cgtgacgatg cgcgtaatcc
     1261 aaggcgcctg cgaccgacct cgtaattgcc acggcgcggt cgtcgctcat cggccctgac
     1321 gcggcgagcg ccttggccag gttgccctgc tcgaagtaag gcatcgaaaa ccacggcacg
     1381 ccttcctctt cccccgcatc gaggacggtg acgatgcccg gatgctccag gcgccgcatg
     1441 tgttgggctt cgtcaaggaa ccgtttacga gcggcgaggc tctttgccag gtccgctctg
     1501 agcactttga gcgccacggg cttccgatgg gtggggtcgc gcgcgagcag aacgaccccc
     1561 atcccgccct gaccgatcgc ttgcagaacc tcgtagcgat cgagccgtcc agccgtgccg
     1621 ggccgggcag gtgccgacag cgcctcaggc gagagcagtt ggtggagggt atcgtagaag
     1681 ctcgaaaccg gtatgtccgg ggaatcgctt gtgctgggag tgctcggttt gttcatggct
     1741 ggttcgccaa gccgtgtcca ttcaatctaa gccacggatg gatgacggat tgagtcgctt
     1801 gcgattcgtt cgccggaccg ttttgcagtc actgaccagt ggccgaaaac ggccctcgac
     1861 tcacagcgcc aaacgcttcg gctgatccgt gtcccatccg tgtttcattc gtggccgctg
     1921 aatcggtgcg gctaaacgcc gccgcctgtt gcctttgtca cgctcgcccg cgcttgctcg
     1981 agcgtcgcct tgctgctctg gtccacccac ggccgagcca gggcccagtc cagcccccgc
     2041 atcaggtccc cgcggcgcag tcgggtgacg agcgttccca ggcgggtgac ggcgacggcg
     2101 atggccaggt agtaaagcac gccggcgacc tcgcggggca ccggggagtc tttcgacttc
     2161 aggcacgcct tcgcgaatct cgcgatcatg gccagaagtt cgatcggcgg cacgggatgt
     2221 tgcatcagcg cgcgcacgct cgacaccgtc aacccctgcg ccacagcaag atcccgcagg
     2281 gcgcgtgccc ggtgagcgct catggcgccc agttcgaact cgatcggagc cgacaggtga
     2341 tagtcccagg ccgccgccag ctcctccggg cgccagggac gagccgattc caatcccgct
     2401 tccaggagat cggcaaagcg cgtctcgcag gcttctccag aaggataggt cttctcggaa
     2461 cacatgtccg cattcttcta cgccgtccgc acgcgcttcc tgcaaatcac ctctgccaga
     2521 aatcccacct gtggggtggt cgcggggttc ctgccgaccc gcgggtgaga ttcccgagtc
     2581 tctgaaacct gcgccccgct catctctctc gtgagaagac gctcatcagt ctcagcagtt
     2641 cgtcatcgac ctcgctttcg ctctcggcat agcgcgcgat cgcgcggcgc agcatcctgc
     2701 caaaccctcg ctttgcggtg acgattgcat tgctggcttg gcgggccgtc gcaaaaccgt
     2761 ggcgccgagc cagttcctcg tccgtcggac gctcgacgcc gtccctggtc ggaacgaacc
     2821 agccttcgta aaaagcgtcc catgtatcca ggcgcttctt gacctcgtaa aaccggcgcg
     2881 tgtcgttctc ggcctcctcg atcacggtga gggcccacag gcgatcgaac gggtcgccgt
     2941 cccggcgcga aacggtccgt tccgcctcct gcagattgtc atcgagcgac accagaccgc
     3001 ccgacggtcg gcgtccagac cgcatttggg cccggtgacc atccttcgca aaattgtcca
     3061 ggacattgag cagaaaggtt cgaaaacgcc cgcgcgagcg gtcggctcgg cccagaaggc
     3121 gtccctccag gaccttacgg gccataaagt cctggagcac gtcctcggca gcttccctcg
     3181 acaacgtgaa tttgtattcg aggtgggcga gcagtggagc gtaatacctg gtcagcagcc
     3241 ggttgagagc gccgtctccc cccatctcct ccggtcgcgt cgccagagaa atcgatgacc
     3301 aatggctcga gggaaacgtc ccctcgcgct cttcgccaac tctcgcccga tgaccttgca
     3361 cgcttttgca ggaaatccga tccgccatcg taaagcaagg tgaaaagcaa atgaagacta
     3421 atggatctgc ggctaatcgc gggagtccct ctctcctgaa tattctcatc cttgttccag
     3481 cgttactgtt gtggctcgcc tcgattccct tggccacagc acaagacgca tacgagcccg
     3541 acaacaccgc cgacaacgcg aaggtcatca ctaacggcca gacccagaac cgatcgatcc
     3601 atcaagccgg caatgtcgat tgggccaagt tcactatcgg cgccagcggt gccaccaacg
     3661 tccgcatcga aacctccggc gccagcggcg acaccgaaat gtggctctac gacccgacca
     3721 gatcccccat gcaactggcc tacgacgacg acaatggcaa tggcagattc tccctgatca
     3781 cgctggcgag cctcgcagcc gggacgtact acatcaaaat caccgaatac ggcaacaacg
     3841 gcacgatcgc ggcctacact ttgagggcta gttggacaga aggtggaggc ggtcagccgg
     3901 atttgacgaa ggagagtgac aacatcagcc gcaccttggg ggcgccggga gacaccttca
     3961 cgatcacgca acgtgtgcgc aacggcggaa ccgccgcgtc gggagccacc aaagtttact
     4021 actacgtcaa caaggattcg cggagcacag cggcggcggc gaaggtgggg gagcgggacc
     4081 ttggcgccct gggcgcgggt tcggtctcag gggagatcac cttcccgtgg acggcgccag
     4141 ccaacctgcc ggcgggcaac tacttcatcg gctactggat tgacgggcag caaagcgtgg
     4201 ctgaaagcaa cgagaacaac aacatttggt ggtgggacaa catcaccatt caaggcgcca
     4261 actggcagtc tgaagc
//
LOCUS       DAOQRO010000306         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22308_length_4260_cov_4.307015, whole genome shotgun sequence.
ACCESSION   DAOQRO010000306 DAOQRO010000000
VERSION     DAOQRO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22308_length_4260_cov_4.307015"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKK20_10825"
     CDS             complement(<1..181)
                     /locus_tag="PKK20_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00422.1"
                     /translation="MSHDFPDPDAIGSMYGLQHLVQSAFGIEARIAYSGIIGRVENQA
                     MVRTLRIRMHRFHPVL"
     gene            complement(171..659)
                     /locus_tag="PKK20_10830"
     CDS             complement(171..659)
                     /locus_tag="PKK20_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00423.1"
                     /translation="MMIRVLDFWLDLEVDEFRLDAVPCLYERKETSCEGFPNPPRLSG
                     GVDFVARGGAGLPIPIATEPIPGMVDALSAVLDALGQYYDQVGTLDAKAQQPPPPTAA
                     RDSATQHPAPAGRIHLTIGDSRHDNARKRSPSQLCGSRPHLARVPRRASLGLVAAPDH
                     ES"
     gene            934..1959
                     /gene="nadA"
                     /locus_tag="PKK20_10835"
     CDS             934..1959
                     /gene="nadA"
                     /locus_tag="PKK20_10835"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNV00424.1"
                     /translation="MNEVQDSKPSGLDRCAGGQSLAPAQAHSLALEIRALKQRLNAVI
                     LAHNYQVPEIQDVADYVGDSLGLSQQAAATPADVIVFCGVHFMAETAKILNPNKIVVL
                     PDKDAGCSLEESCPPDQLAALQRTNPNFYTVTYINCSAAVKALSDVICTSGNAVKIVE
                     ASPKEKDLLFVPDENLGAWVMEQTNRPMTLWKGNCYAHVEFRRDSLLRMRQRYPDAKV
                     VVHPESLREVRDLGDAVCSTEKMISYCKTSPARQFIIVTESGIVHRLRKECPGKEFLC
                     APVFDAMRLPTDNCRCCECKYMKMNTLEKVRDCMKHLAPRIELPDDIRQRARLPIERM
                     LEISARP"
     gene            complement(1985..3298)
                     /locus_tag="PKK20_10840"
     CDS             complement(1985..3298)
                     /locus_tag="PKK20_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00425.1"
                     /translation="MPRKHLPVHGEETVGGHGAVTQQTFGGLSWDHPGWRSGTSDQSD
                     QSDQSDRSAKPETEDVGSEARASLIYEMQTDSAYGYANWSAGQDPDLLDAYPAEELVR
                     IRQAKVPRDWDTRWRAAAHKVGFDGVAKEPKVALKSSPIWVALPRFERAFPPFDFNSG
                     MWGEDVDRESAVRLGLITISEQVRPQEKPFAENWQTSVENLSKELLDSLLRAFPDRVK
                     IVGDKAAWTDRSESSKILPSTREKQQSKLAMPYEHRESTEQDACQMADSARTLKSAEA
                     NARGSGSPAPAHDEGRSERIARIRGEERRLGEWAEREGLIAQELPTADDLYGEHGVHY
                     DQKSGRYIKFTRHDRHQGYGIALSSYLHGASPAEYLDRIRLQNQIFSDDIRLEGIVKM
                     AGGRLSIITSQPRIVGEAPTLNDIDVLQVNTAFVGDGAVTAPLHK"
     gene            3474..3896
                     /locus_tag="PKK20_10845"
     CDS             3474..3896
                     /locus_tag="PKK20_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNV00426.1"
                     /translation="MALKIERIDTWAASLEDNPGNLAAKLNALAEAGVNLEFVMARRA
                     PDKPGTGVVFVTPIKGAAGCRAARQVGFKKTQSLHAVRIEGPDKPGQGARITQALGEK
                     GLNLRGLSAAAVGKNCVTHIAFDTAADAGKAIRIVRGL"
BASE COUNT          797 a         1315 c         1302 g          846 t
ORIGIN      
        1 gcaacaccgg gtggaaccgg tgcatccgga tgcggagggt ccggaccatc gcctggtttt
       61 cgacgcgccc gataatcccg ctgtaggcga tgcgggcttc gatgccaaaa gccgattgca
      121 ccaggtgctg aagcccatac atcgagccga tcgcgtccgg atccggaaag tcatgactca
      181 tgatcaggag cggcgacaag gccgagcgat gctcgcctag gaactcgagc aaggtgcggg
      241 cgtgatccgc agagttggga gggggaccgt tttcgggcgt tgtcatggcg agagtctcca
      301 atcgtcagat ggattcgccc agcaggcgca ggatgctgcg tagcggaatc tcgagccgca
      361 gtgggcggcg gcggttgttg ggctttggca tcgagcgttc ccacctgatc atagtattgt
      421 cccaaggcat ccagcacggc gctcaacgca tccaccatgc cgggaatcgg ttccgtggcg
      481 atcgggatcg ggagccccgc tccgccgcgg gcaacaaagt ccacgccgcc cgacaaacgc
      541 ggcgggttgg gaaacccctc gcaactcgtc tccttccgtt cgtacaggca cggcacggcg
      601 tccagccgaa attcgtcgac ttcgaggtcg agccagaaat ccagcacccg gatcatcatg
      661 attgatcaac ccgccgcgcc cgctctggcg cgcatccccg atgcctgcgt ctcggcggca
      721 gacagtcggg cgccacgacg ccttcgccct tgccttccgc gcgtcctcga aaccgagcgc
      781 accgaggcct gcgaccagcg atcccgcgcg tctggtttct gccaagcacg cgcggatact
      841 cggacgggcg atccgcggga tgcaacaggt ttttgcggag ccgaagcggc ccgctcaatt
      901 gacgtccgat ccggtccgcg ctagcgtgcc cccatgaatg aggtacagga tagcaagccg
      961 agcggcctcg atcgttgtgc ggggggccag tccctggcgc cggcacaggc acactcgctt
     1021 gcgctcgaaa tccgcgctct caagcagcgc ctgaacgccg tcattctcgc gcacaattac
     1081 caggtgcccg agattcagga cgtcgccgat tacgtcggcg attcactcgg cctgtcgcaa
     1141 caggcggcgg cgaccccggc cgacgtcatt gttttctgtg gcgtccattt catggccgag
     1201 acggccaaaa tcctgaatcc caacaagatc gtcgtgctgc cggacaagga cgccggatgc
     1261 tcgctcgagg aaagctgtcc gcccgaccag ttggccgcat tgcagcggac caatccgaac
     1321 ttctataccg tcacctacat caactgcagc gcggcggtga aggcgttgag cgacgtgatt
     1381 tgcaccagcg gcaacgcggt caagatcgtg gaggcgagtc cgaaggagaa ggaccttctg
     1441 tttgtgccgg acgagaatct gggcgcctgg gtcatggagc aaaccaaccg gcccatgacc
     1501 ctgtggaagg gcaattgcta cgcgcacgtc gagttccggc gcgacagcct gttgcggatg
     1561 cgtcagcggt atccggacgc caaggttgtg gtgcatccgg agagcctgcg cgaggtgcgc
     1621 gatcttgggg acgcagtctg ttcgacggag aagatgatca gctactgcaa gaccagcccc
     1681 gccaggcagt tcatcatcgt caccgaatcg ggcatcgtcc atcgcctgcg gaaggaatgt
     1741 cccggcaagg agttcctctg cgccccggtt ttcgacgcga tgcggctgcc caccgacaac
     1801 tgccgctgct gcgaatgcaa gtacatgaaa atgaacacgc tcgagaaagt gcgcgactgc
     1861 atgaagcatc tcgcaccgcg catcgagttg cccgacgaca tccgccaacg ggcccggctc
     1921 cccatcgagc gcatgctcga gatctcggcc cgcccctgat ccggtcttcg acttttgtca
     1981 gcgctcattt gtgaagtggg gcggtgacgg ccccgtcgcc gacgaacgcc gtattcactt
     2041 gaagtacgtc aatatcgtta agcgtagggg cctcgcctac gatgcgcggc tgggatgtga
     2101 ttatagacaa tcttccacct gccattttaa cgatcccctc cagcctgatg tcatcgctaa
     2161 aaatctggtt ttgcagcctg atcctatcca agtattcagc agggctcgcc ccatgaagat
     2221 agctgcttag cgcgattcca tatccttgat gcctgtcatg gcgtgtaaat ttgatgtacc
     2281 tgccggattt ttgatcgtag tggactccat gttcaccgta cagatcatct gctgtgggca
     2341 actcttgcgc tatgagccct tcacgctcag cccattcacc gagtcgtctc tcctcgccgc
     2401 ggatccgcgc aatgcgttct gatcgtccct catcatgcgc tggcgctggg ctgccacttc
     2461 ctctggcgtt tgcttcggct gatttaagcg tccttgcaga gtcggccatt tgacatgcgt
     2521 cttgttctgt tgattctctg tgctcataag gcatcgcaag tttcgactgt tgtttttccc
     2581 gcgtcgatgg gagaatttta cttgattcgg aacgatctgt ccacgccgcc ttatcaccaa
     2641 cgattttgac ccggtcaggg aacgctcgca gcagcgaatc gagcagctcc ttcgagagat
     2701 tctcgacgct ggtctgccag ttctcggcaa acggcttctc ctggggccgc acctgctcgc
     2761 tgatcgtgat cagcccgagc cggaccgccg actcacggtc cacatcctcg ccccacatgc
     2821 cgctgttgaa atcgaatgga gggaacgcgc gctcgaatct cgggagcgcc acccagatcg
     2881 ggctgctctt gagggcgacc ttcggttcct tggcaacgcc gtcgaagccg actttatgag
     2941 cggcggcgcg ccacctggta tcccaatccc gcgggacctt cgcctggcga atgcgcacga
     3001 gttcctctgc cgggtacgca tcgagcaggt cagggtcctg gccggccgac cagttcgcgt
     3061 aaccgtatgc gctgtcggtc tgcatctcgt agatgaggct cgcgcgcgct tcgctgccga
     3121 cgtcctcggt ctcgggtttg gcagaccgat cggactggtc ggactggtcg gactggtcgg
     3181 acgtgcctga cctccatcct ggatggtccc acgacaggcc gccgaatgtc tgttgagtga
     3241 cggcgccgtg gccgccgacg gtctcttcgc catgaacggg caggtgtttt ctcggcaaac
     3301 gcaggcaaga actctgcgcg tccttgatca gaattgtggt agtgattccg ctcgctccaa
     3361 cgtccggcga tctcccccac agaatcgaaa acgcgttgac cggtcaggcc tgcgagtcta
     3421 tgatcgccga ggttggaaaa gaagctatcc ggcaactgaa tggaggatgt gttatggcac
     3481 tgaagatcga acgaattgac acctgggcgg cctctctgga ggacaacccc ggcaaccttg
     3541 cggccaagct caacgcgctc gcggaagcgg gagtgaatct ggagtttgtc atggcgcgtc
     3601 gcgcaccgga caaacccggc accggggttg tctttgtgac cccgatcaaa ggcgcggctg
     3661 gatgtcgggc ggcacggcaa gtgggcttca agaagaccca gagcctgcac gctgttcgca
     3721 tcgaaggacc agacaaaccg gggcaaggcg ctcggatcac gcaagccttg ggtgagaagg
     3781 gactgaactt gcgcgggctt tcggccgccg cggtcgggaa gaactgcgta acgcacattg
     3841 cgttcgacac ggcggcggat gccggcaagg ccatccgcat cgtgcgcgga ttgtagggga
     3901 cggtcgctcg gagcgcttga atttggggcg gcagtggtga ccatgcgcaa agcgatgctt
     3961 ctttgggttg cgtcgatgtt cggcgtgctt tcgcacgatg gcattcgaac ctcctggacc
     4021 acgagcacgg cttgaggcgc ttggatcgac cgattcgctt cctccttgtg cagcgaggcg
     4081 ccttgcgccc cgatgctgaa ttccagccca tcccggttcc agccccccac gactggcgga
     4141 cagcgcgtcc tgagccgatg aaaggtcgcg cgtcgagcaa gcggttgccg agtgattctg
     4201 gccggcctgc caccggcgag atgaaactgg cccaaaacgg gcggtttgaa atgggatctt
//
LOCUS       DAOQRO010000307         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22487_length_4237_cov_3.916308, whole genome shotgun sequence.
ACCESSION   DAOQRO010000307 DAOQRO010000000
VERSION     DAOQRO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22487_length_4237_cov_3.916308"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1802
                     /locus_tag="PKK20_10850"
     CDS             189..1802
                     /locus_tag="PKK20_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00427.1"
                     /translation="MKPPTLTISLALSTALTALILTFTQQAQAQSTTVEDPLHGRRHF
                     NVPPGDFYTGRSPFTGGEMAPAAPRPARVVERPAAPAAPVEPIGGCQVIDTGWVHMTK
                     TMPREVVLGQEFMYQLDPRAVACVGNVVVTDQIPPGTTYVRSEPQAEVVGDRLVWKLG
                     DMDPGQIVPLKVWVRADREGEVGSCATVSADPRLCAKTLVGRPLLAIEKTGPEIAQLN
                     SDVAYNIVVANRGNAVARNVVVTDDIPEGLTHAGGQKQLTFNVGDLAPNQSKTIPVTL
                     KATQRGKFCNAASAVADNVPKVSDDACTTVVQPGLKIVKTGDERQLINKTANYQIKVS
                     NVGDTDLSGVVVTDTAPSPTAIVAAQDATVSGNTATWNIGTLPKGAEKTFTVSLTSPE
                     PGNYCNGVSVATAQGLRESAQACTEWIGVTGVLVEVVDDPDPIQVNEFTTFTIRVTNQ
                     GSTRNIEDINVKAMFQAEADPVTASSGGTVSGKNVSWPVLPTLAPKASVTYTLRAKGV
                     AAGDHRLEVQVTTRNRENPIIELESTTVY"
     gene            1938..3257
                     /locus_tag="PKK20_10855"
     CDS             1938..3257
                     /locus_tag="PKK20_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV00428.1"
                     /translation="MRTLHWYLLRQVMASVVMTVLVFTFVLLLGNVLKEILGLLVNRQ
                     ATLGIILEAIALLVPCVLVYALPMGLLTATLLAFGRFSADQELTAARASGISLIALAA
                     PVLLLSLVLSGLSGWFNLHLAPQCRMAYKELFYRLGIEKPTTLLAENQFIKDFPGYVI
                     YVGSARGNTLKNVVIYQMETNRPPPSPDGTENPAILPETPALTNEAPVTAIATNEPPT
                     TPIATNASPDTADATNAAPDLDAHPPSTPDSPPRSNLSPSIPRVELVLSAPEATVSID
                     STDGQVRLFLPEVEGVYRDSWQLGGARDRTLLLPVRLNAPRRETLRLSEMTFHRLMQE
                     YYDYRRRGVDTTPVAVQMHRQVAFSFACIGFALIGIPLGVRAHRRETSAGVAIALVLV
                     LLYYSFIILGQAWETRPERLPHLVLWIPNLFFEAVGAALLWRVNRRG"
     gene            complement(3311..4042)
                     /locus_tag="PKK20_10860"
     CDS             complement(3311..4042)
                     /locus_tag="PKK20_10860"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HNV00429.1"
                     /translation="MPDTRSHRGPAPEDERLFDAKSWPVLRAAIDELCWLLDRGYAPH
                     SAAELVGNRHALTARQRMAVSRSACTDEARERRRQHEVESSRLGAQELWIDGLNVLTG
                     VEAALAGGVLLLGCDGCLRDLASIHARHHHVEETLPALHWIGVTTNAWGVAGCRWWLD
                     RPVANTGRLKGTMLEMAAANGWAWEVELVYNPDKVLIESPAIVASSDSAILDRCGRWV
                     NLARHTIERHVPTARIIDLRRTIGL"
BASE COUNT          794 a         1508 c         1194 g          741 t
ORIGIN      
        1 tcgtggtttg tcgagatcat cgcacgccta ctacgcccca aaccacggca attgataagc
       61 gctctttcca acctccacaa cagcaaccct ttcaggagtt taaatgaaat gaccgtgccc
      121 cttgtgtttg tgacgccgat gtggaatttg acacgctgag cagaatgggc ttaaactccg
      181 cccgagaaat gaaacctcca acactgacca tctcgttggc gctgtcaacc gccctgactg
      241 cgctcattct aaccttcaca caacaggctc aggcgcaatc gacgactgtc gaagatccgc
      301 tgcacggtcg tcgccatttc aatgtgcctc ccggcgattt ctacacaggc cgctccccat
      361 tcaccggggg agagatggcc ccggcggcgc ccagacccgc ccgcgtcgtc gaacgccccg
      421 cggccccagc cgctccggtc gagccgatcg gcggttgcca ggtgatcgac accggctggg
      481 tgcacatgac caagaccatg ccgcgtgaag tcgtgttggg tcaggagttc atgtaccaac
      541 tcgatccgcg cgcggtggcc tgcgtgggca acgtcgtggt cacggaccag attccccccg
      601 gcacgaccta cgtccgaagc gagccccagg ccgaggtggt tggcgatcgt ttggtgtgga
      661 agttgggcga tatggacccg ggacagatcg tcccgttgaa ggtctgggtg cgcgccgatc
      721 gcgaaggcga agtgggctcc tgcgcgacgg tttcagccga tccgcgcctt tgcgccaaga
      781 cgctcgtcgg aaggccgctc ctggctatcg aaaagacggg gcctgaaata gcccaactca
      841 actccgatgt cgcctacaac atcgtcgtgg ccaatcgcgg caacgccgta gcccgcaacg
      901 tggtggtgac cgatgacatc cctgaaggcc tcacgcacgc cggcggccag aaacagttga
      961 ccttcaatgt cggcgacctg gcccccaacc agtccaagac aatcccggtc acgctcaagg
     1021 ccacccagcg cggcaagttc tgcaacgcgg cctccgcagt ggccgacaat gtcccgaagg
     1081 tcagcgacga cgcctgcacg accgttgttc agcccggtct gaagatcgtg aagacgggcg
     1141 acgagcggca attgatcaac aagacggcca actaccagat caaggtcagc aatgtgggcg
     1201 ataccgacct cagcggcgtg gtcgtgaccg acacggcgcc gtcgccaacc gcgatcgtcg
     1261 ccgcccagga cgccacagtg agtggaaaca ccgcgacgtg gaatatcggc accctgccca
     1321 agggcgccga gaagaccttc accgtctcgc ttacatcccc cgagccgggc aactactgca
     1381 acggagtctc cgtggcgacc gctcagggcc tgcgtgaatc cgcccaggcc tgcaccgagt
     1441 ggatcggtgt gacgggcgtc ctcgtcgagg tggtggacga tcccgacccg atccaggtga
     1501 atgaattcac cacgttcacc atccgcgtga ccaaccaggg ctcgacccgc aacattgagg
     1561 acatcaatgt caaagcgatg ttccaggccg aggccgatcc ggtcaccgca tcgagcggcg
     1621 gaaccgtcag cggcaagaat gtctcgtggc cggtcctgcc cactctggcg cccaaggcct
     1681 cagtcaccta cactctccga gccaagggcg tcgccgccgg tgaccaccga ctcgaagtcc
     1741 aggtgaccac gcgcaaccgg gagaacccga tcatcgaact cgaaagcacg acggtttact
     1801 aaccgtcgtg gctgtccggc ccgcccccga tcgaggcggc ggccggaccc gcctctctcg
     1861 gggccgcctt cattcgggaa ggcggccctt ttcttttgcc ggaaaccgcg ccaagataac
     1921 cgcgctccgc aagccggatg agaacgctgc attggtatct cttgcgccag gtgatggcga
     1981 gtgtcgtgat gacggtcctc gtcttcacct tcgtgctctt attgggcaac gtcctcaagg
     2041 agattctcgg actgctcgtc aaccggcagg ccactctggg catcatcctc gaggccatcg
     2101 ccctcctcgt gccttgtgtc ctggtctatg cgctcccgat gggcctgctt accgccacgc
     2161 ttctggcctt cggacgcttc agcgccgatc aggagttgac agccgcccgc gccagcggca
     2221 tcagcctcat cgccctggcc gcgccggttt tgctcttgag cctggtgctt tccgggttga
     2281 gcggttggtt caacctccac ctcgctcccc agtgccgcat ggcctacaag gaattgttct
     2341 atcgcctggg catcgagaaa ccgaccaccc tgctcgccga gaaccagttc atcaaagact
     2401 ttcccggcta tgtcatctat gtcggcagcg cccgcgggaa cacgctcaaa aacgtcgtca
     2461 tctaccagat ggagaccaac cgcccacccc catcgcccga cggcacagaa aacccggcga
     2521 tcctccctga aacccccgcc ctaacgaatg aggcgccggt taccgccatc gcgacgaatg
     2581 agccgccgac caccccaatc gccacgaatg cctcccccga cactgcggac gccacgaacg
     2641 ccgcgcccga tctggacgcc caccccccct cgacgcccga ctcaccgccc cgcagcaacc
     2701 tctccccatc cataccgcga gttgaactgg tgctgagcgc gccggaggca accgtgagca
     2761 tcgactccac cgacggacaa gtgcgcctgt tccttccaga ggtcgagggc gtttaccgcg
     2821 attcctggca gttgggcgga gcccgagatc gcaccctcct cctgcccgtc cgcctgaatg
     2881 cccctcgccg cgagacgctc agactctccg agatgacatt ccacaggctc atgcaggagt
     2941 actacgacta tcgccggcgc ggcgtcgata caacgcccgt cgccgtccaa atgcatcgcc
     3001 aggtcgcatt ctcgttcgcc tgcatcggat ttgccctaat cggcatcccc ctgggcgtgc
     3061 gggcgcatcg gcgcgagacg agcgccggcg tcgccatcgc cctggtcctg gtcctcctct
     3121 actacagctt catcatcctt ggccaggctt gggaaacgcg gcctgagcgc ctgccgcatc
     3181 tggtcctgtg gataccgaac ctgttcttcg aggccgtggg cgccgccctc ctctggcggg
     3241 tcaaccgccg cggataaccc acaagccgct ggggtcaaat ctctggttta gatctgaccc
     3301 gctcgccgga tcagagaccg attgtccgcc tcagatcgat gatccgcgct gtcggcacgt
     3361 gccgctcgat ggtgtgccgg gccagattca cccagcgtcc gcaacgatcg agaatcgcac
     3421 tgtccgacga tgcaacgatc gccggggact cgatcagcac cttgtccggg ttgtagacca
     3481 actcgacctc ccaagcccag ccattggccg ccgccatctc gagcattgtc cccttgagac
     3541 gcccggtgtt agccacgggc cggtccagcc accaacggca gccggcaacc ccccaggcgt
     3601 ttgtcgtcac cccgatccag tgcagcgcgg ggagcgtttc ctcgacgtgg tggtggcgcg
     3661 catggatgct cgccaaatcc cgaagacaac cgtcgcagcc cagcagcaga actccgcccg
     3721 ccagcgccgc ctccactccc gtgagcacat tcaatccgtc gatccacagt tcttgcgctc
     3781 cgagtcggct cgactcgact tcatgctgcc gacggcgctc acgcgcctca tccgtgcacg
     3841 ccgagcgcga caccgccatc cgctgccggg ctgtcagggc gtgccggttg ccgaccaact
     3901 ccgccgcgga atgcggggcg tacccccgat ccagcagcca gcacagctcg tcgatggccg
     3961 cgcgcaacac cggccaggat ttcgcgtcga acagacgctc gtcctccgga gcgggaccgc
     4021 gatggcttcg tgtgtccggc actcttccct ctctgtgtct ttgtggcgaa ccctattcga
     4081 ccacgggcag cgtcctggat tgaaccacgg agacacagcg ctcgcaaacc gtaaccagag
     4141 gtcggagagc cgctttagat gcgaccccat tgccgttcag gaatcgcgga cggtcgcctc
     4201 caggtcccgg cacaatcgct ccacgatctt ctcttgc
//
LOCUS       DAOQRO010000308         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22495_length_4236_cov_4.841665, whole genome shotgun sequence.
ACCESSION   DAOQRO010000308 DAOQRO010000000
VERSION     DAOQRO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22495_length_4236_cov_4.841665"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKK20_10865"
     CDS             complement(<1..362)
                     /locus_tag="PKK20_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HNV00430.1"
                     /translation="MKSPFPGMDPYMESHWGDVYHSLIQSIYKIRAILLVCVLLPLSA
                     LDVQAALRAGAAVVDATPPHLPVLVNGGFFSRSADTVRTPIQCGALVLDDGRERVAIV
                     IADSCMMPRSLLDEAKQLA"
     gene            577..1644
                     /locus_tag="PKK20_10870"
     CDS             577..1644
                     /locus_tag="PKK20_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV00431.1"
                     /translation="MSEVLDHGGPGYLQFAITNLCNAKCGFCGFAVDRFNPRQRRSVT
                     LDEAREVIDICVPNHIGYLLFVGGEPMVHRDLGSMVRYAAERGVRPMICTNGSLFTDA
                     NMLELARNGLTGVIMSIDAPDVARHERNRGLPGVCEKISRANAAFAKLGVQSTASVTV
                     SRLIEDYSKLPAFLRSLGFTSCTFSYPLTTLGSTYLSFSDSQLVAYRKEELIEVFEQI
                     KRMKRESGFPIVNPSESLTEMQRHLRGETERFGCLGGHKYFYLDWNLDLYRCHYWETP
                     MCPIREFNRSKLIRDGCTRCMIDCYRDPSVLQFIAVNACDAWQSLRRGRCLAAARHIC
                     DPRNLTSLRAVWENRAWIGDV"
     gene            complement(1729..2838)
                     /gene="purM"
                     /locus_tag="PKK20_10875"
     CDS             complement(1729..2838)
                     /gene="purM"
                     /locus_tag="PKK20_10875"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNV00432.1"
                     /translation="MKQKAYAAAGVDIDLGNRVKSKLPQLLAATRRPGQLGKVGGFGG
                     LFALDIRRYRNPVLVASVDGVGTKLKIAFALNRHDTVGQDLVNHCVNDIAVLGAEPLF
                     FLDYIGTGKLEPDVFTEIVKGFACACAANRCALIGGETAQMPGFYQPGEYDVSGTIVG
                     VVDRSAMLDGTKTVRVGDAVIGLASSGLHTNGYSLARKIFFEQLKLKPTSRIADFEGT
                     VGDELLKVHLSYGPLVRALLQEFNRRPRTPAAVGGARGGSRRNAWSGGAIKAFAHITG
                     GGFVDNIPRILPKTCDVVIRKGTWDVLPVFRVLADKGKIPEAELYQVFNMGIGMVAVV
                     AADQAEAVLRRCGRHGHRATLIGEIVRGRGETRIE"
     gene            complement(3219..3659)
                     /locus_tag="PKK20_10880"
     CDS             complement(3219..3659)
                     /locus_tag="PKK20_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00433.1"
                     /translation="MRTAIFKPAGALVGYLLQGAADRIDAAYQSKPGQVYKGRVTVRI
                     QCLFGCFELQRDYYYHECKQQGHYPADAALGLEVGYTPALARLLCLEGADENSYQKAQ
                     LHLAETGGIHVSGRQIQRVVQRVGPDAQKWQDVESDGAKGEGRH"
     gene            complement(3719..4072)
                     /locus_tag="PKK20_10885"
     CDS             complement(3719..4072)
                     /locus_tag="PKK20_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00434.1"
                     /translation="MTATKTALLTQIAQIQLMERGKLSAYTFKERARRATPYYKLQAW
                     ENGKNVTRYIRSEQVALVEEALAGHVKFQELVGQYAQLVIDQTREQLAAVGVKRKPGR
                     RPSSSSSRRSRKSSN"
BASE COUNT          791 a         1353 c         1255 g          837 t
ORIGIN      
        1 gccagttgtt tggcttcatc caagagcgaa cgcggcatca tgcaactgtc cgcgatgacg
       61 atcgccacac gctcgcgccc gtcatcgagc accagcgccc cgcactgaat cggcgttcga
      121 accgtgtcgg ctgagcgcga gaagaaaccg ccattgacca gaacgggcaa gtgaggcggc
      181 gtggcatcca caaccgccgc cccagcccgc agggcggcct ggacatcgag cgcgctcaaa
      241 ggcaacagga cacaaacgag aagaatggca cggattttgt atatggactg gatgaggctg
      301 tgatacacat cgccccaatg cgactccatg tacgggtcca tgcctgggaa tgggctcttc
      361 ataagacgga ccttatccca ttacgcatcg cctgtcaccc ctccgaggat catctgaaga
      421 aacatttgct ccgacggcaa ttcaccggca ctctgtcgct cctgctgcat gagccgcgtg
      481 cccgaaccca gctcttcatc gacacaggtg tcgcctccga ccgggatcga ccgattgcct
      541 ccggtcacgc cgccgcacaa gagtcttccg agactcgtga gcgaggtcct ggaccacggc
      601 ggcccaggct atctgcaatt cgccatcacc aacctctgca acgccaaatg cggcttctgc
      661 gggtttgccg tggaccgatt caatccgcgg cagcgacgca gtgtgacgct cgacgaggcc
      721 agggaggtca tcgacatctg cgttcccaac cacatcggct acctgctctt cgtcgggggc
      781 gagcccatgg tgcatcggga tttgggatcg atggtgcgct acgcggcaga gcggggtgtg
      841 cgaccgatga tttgcaccaa cggttcgctc ttcacggacg ccaatatgct tgagttggcc
      901 cgcaacggcc tgaccggcgt catcatgtcg atcgacgccc ccgatgtggc gcggcatgag
      961 cggaaccggg gccttcccgg cgtgtgcgag aagatcagcc gcgccaatgc ggcgttcgca
     1021 aagctcggtg tgcagagcac ggccagtgtg accgtgagtc ggctgatcga ggattactcg
     1081 aagctcccgg ccttcctgcg gagcctgggt ttcaccagtt gcaccttcag ctatcccctg
     1141 acaacgctcg gctcgactta cctgagcttc agcgactcgc agctcgtggc ctaccgcaaa
     1201 gaggaactga tcgaggtctt cgagcagatc aagcggatga agcgcgaaag cggattcccg
     1261 atcgtcaatc cgtccgagtc gctcactgaa atgcagcggc atttgcgcgg cgagacggag
     1321 cgattcggct gcctgggcgg acacaagtac ttctacctgg attggaacct ggacctgtac
     1381 cggtgtcact actgggaaac gcccatgtgc ccgattcgcg aattcaaccg gtccaagctc
     1441 atccgggatg gctgtacccg ctgcatgatt gactgttacc gcgatccgag cgtgctccag
     1501 ttcatcgccg tcaatgcatg cgacgcctgg cagagtctca ggcgcggacg ctgcctcgcc
     1561 gcggccagac acatctgcga tccccgcaat ctcacatccc tcagggccgt ctgggaaaac
     1621 cgcgcgtgga tcggcgatgt ctgaaccgat gggacgcagt tcgggctagc ccggaccagg
     1681 ttgagcgcgg agcctttgag cgatccgggc caacgccgcc gcgtacaatc actcgatccg
     1741 cgtttctcca cggccccgca cgatttcgcc gatcaatgtc gcgcggtgtc cgtgtcggcc
     1801 acagcggcgc aaaaccgctt ccgcctggtc cgcagcgacc acggccacca ttccaatgcc
     1861 catgttgaag acctggtaaa gctcggcttc gggaatcttc cccttgtccg ccagcacccg
     1921 aaagaccggc agcacgtccc atgtcccctt ccggatgacg acgtcacatg tcttcggtag
     1981 aatgcgcggg atattgtcga caaagccgcc gcccgtgatg tgcgcaaagg ccttgatcgc
     2041 acccccggac caggcgttgc gccggctgcc gccccgagcg ccgcccaccg ccgcaggcgt
     2101 cctcggcctg cgattgaatt cctgaagcaa cgcgcggacc agcggaccgt agctcagatg
     2161 gaccttcagc agttcatcgc caaccgtccc ctcgaaatcc gcgatccggc tggtgggctt
     2221 cagcttcagt tgctcgaaga agatcttgcg ggctagggaa taaccgttgg tgtgcagccc
     2281 gctcgatgcc agaccgatca ccgcgtcgcc cacacgaacg gtcttcgtgc catcgagcat
     2341 cgcggaccga tcaaccacgc cgacgatggt cccgctgaca tcgtactcgc ccggctggta
     2401 aaaaccgggc atctgagccg tctcgccgcc gatgagagcg catcggttcg cggcgcaggc
     2461 gcaggcgaag cccttgacaa tctcggtaaa gacgtcgggt tcgagtttcc cggtcccgat
     2521 gtaatcgagg aagaaaagcg gctcggctcc cagcaccgcg atgtcgttga cgcaatggtt
     2581 gacgaggtcc tggccaacgg tgtcgtgacg gttcagtgcg aaggcaatct tgagtttggt
     2641 gccgacgccg tcgacgctcg caaccaggac cgggttgcgg tagcggcgga tgtcgagtgc
     2701 gaagaggccg ccaaacccgc cgactttgcc gagttggccc ggacgccgcg tggcggcgag
     2761 cagttgtgga agtttggatt tgactcggtt gccgaggtcg atgtcgacgc cggcggcagc
     2821 ataagccttt tgtttcatgt cagaaatcgg gcatccgtgc cgatgcaggt gagaagatgc
     2881 ctccttggaa gaccacaggc gataggaatc tgaagcaacc accctccgct tcacaagccg
     2941 gacaagacat gccatcgtcg gatccacctg cgccggtgga attggtgtta tgatggcggg
     3001 tttgacgacg acctttgaag cgcttgggat tgcgttggga ctcggtctgt tggtcggcat
     3061 gcagcgtgca cgggcggctt cgcgccagtc tggttcaggt tctcattgag ctttgctctg
     3121 tcgttctttg gcccttcgct cctccgggct ttcgcccggt ctctttgcta tgcctctgct
     3181 gacttcccgc cacctctcgg cagcggaatc tccctgggtc agtgccgacc ctctcccttc
     3241 gcgccgtcgg actctacgtc ctgccacttc tgggcgtccg gacccacccg ctgcaccacc
     3301 cgctgaatct gccgccccga aacgtgtatc ccgcccgtct cagccagatg cagttgggcc
     3361 ttctggtaac tgttctcgtc cgccccttcc aaacacagaa ggcgggccag tgccggcgtg
     3421 tagcccactt ccaagcccaa cgcggcatcc gcagggtagt gcccctgctg cttgcactcg
     3481 tgatagtagt aatcccgctg cagctcgaaa cagccaaaca gacactgaat ccggaccgtg
     3541 actctcccct tgtacacttg gcccggcttg gattgatagg cggcgtcgat ccggtcggcg
     3601 gccccttgca gtagatatcc caccagcgca ccagccggtt tgaagatcgc ggtgcgcacc
     3661 aacacttcca gttcctgcac caagctcccc gtgggagctt gcccctggaa gcgcgtcatc
     3721 agttgctcga cttcctgctc ctgcgcgagg aggaggaact ggggcggcgg cccggctttc
     3781 tttttacgcc cactgccgcg agttgttcgc gggtttggtc gatcaccagt tgagcgtatt
     3841 gcccgaccaa ctcctggaac ttgacgtggc cagccagtgc ctcctcgacc agcgcgacct
     3901 gttcggagcg gatgtagcgg gtgacgttct tgccgttttc ccaagcctgc aacttgtagt
     3961 agggcgtggc gcgccgggcg cgttctttga aggtgtaggc cgagagtttg ccacgttcca
     4021 tgagttggat ctgagcgatc tgggtcagga gggcggtttt ggtggcggtc atatatgtga
     4081 ctatataatt agtcacacat ccaccgcaat tcaagccttt tcatcaatcc gaagaattcc
     4141 gtccgacacc ccgcgcgccc agggcggttc tgagctggga gggggtgatt gaggaagagg
     4201 gacttaacga atgtgcggaa caatcctccg cccatc
//
LOCUS       DAOQRO010000309         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22636_length_4216_cov_4.850997, whole genome shotgun sequence.
ACCESSION   DAOQRO010000309 DAOQRO010000000
VERSION     DAOQRO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22636_length_4216_cov_4.850997"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PKK20_10890"
     CDS             complement(<1..176)
                     /locus_tag="PKK20_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNV00435.1"
                     /translation="MPTLDLRPSDACHYDLAALGEIMLRFDPGEGRIRNARAFRVWEG
                     GGEYNVVRGLRKCFG"
     gene            326..1978
                     /locus_tag="PKK20_10895"
     CDS             326..1978
                     /locus_tag="PKK20_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNV00436.1"
                     /translation="MKEAKSVSISPTGEKFPLPSPKDYKKEFARLEALAKARRKQGQE
                     VVVVVGLGFVGAVMAAVVADARDKKSGRPNKLVIGMQRPSPRSYWKIPLLNRGISPVK
                     AEDPEVETLIGRCVKESKTLVATYTEEALRLADVVVVDVQCDYLKESLGDVRTGSADM
                     EALEASIGTIAERIPAGALVLIETTVAPGTTEQVAYPIMKKIFERRRIASEPLLAHSY
                     ERVMPGRNYVASIRDFWRVCSGINDEARDKVVRFLKDVLNTEKFPLTVLDRPIESETA
                     KIVENSYRATILAFLDEWSMFAERNGVDLKKVIEAIRVRPTHSNLIFPGPGIGGYCLP
                     KDGGLGMWAYKHIFGWEDNIFKITPAAININDTRSLHAPQLVRDALRNMGKPLAAAEV
                     LILGAAYREDVGDTRYSGSEVMVRKLAEMGADVKVHDPYVEHWWEFEAQDTYPHPGYS
                     LKRFFHRQDQLVNLRIQPDLKAALSGVDAVVFAVRHQQYLKLDPDAVFRQVGKPFAVV
                     DCFCILSDAQIRRYLELGCDVKGMGRGHIKRIKDSISASKRP"
     gene            complement(2006..3298)
                     /locus_tag="PKK20_10900"
     CDS             complement(2006..3298)
                     /locus_tag="PKK20_10900"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNV00437.1"
                     /translation="MIKPAKLELDGRVYELPTLRGTEGEPAVSIAALRDQTGHITLDD
                     GYSNTGSCRSAITFIDGENGILRYRGIPIEELAEKSTFVETAYLLIYGNLPTRTQFRA
                     FSELLTDNENLHEDMKYHFEGFPSTAHPMAILSAMINASSCFYPGLNGPRNSERFDIH
                     AARLISQVRTIAAFSYRKSRGLPIIYPKRAYKYTANLLHMMFSDPYEDYNLQPEIVRA
                     LDLIFLLHADHEQNCSTATVRMVASSQANLFACAAAGVCALWGPLHGGANQAVVEMLQ
                     EIHKSGDDGSKFIAAAKDKNSGRRLMGFGHRLYKNYDPRATIIKKACDQILKRIQVAD
                     PLLDIAKRLEEAALNDSYFVERKLYPNVDFYSGIIMRAVGIPIEMFTVIFAIGRMPGW
                     IANFKEIMEDPKCRIYRPRQIYVGSGLRHYVPLEQREQ"
     gene            complement(3414..3713)
                     /locus_tag="PKK20_10905"
     CDS             complement(3414..3713)
                     /locus_tag="PKK20_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNV00438.1"
                     /translation="MTGDFLHLQGDEVLLAIRVQPRSSRNELAGAIGPELKVRIAAPP
                     VDDAANEEVLRFLARTLGCPRNQLRLAHGRASRHKRVGIRGFSVDQVRQKLGAVG"
     gene            complement(3710..>4216)
                     /locus_tag="PKK20_10910"
     CDS             complement(3710..>4216)
                     /locus_tag="PKK20_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00439.1"
                     /translation="HLWLLLLSMVNRRVPDTDPWQRLVRQYLGRLEPWPVAVKLTAPI
                     LLVGALWWFAHAELVRQGMTAAPGSAAHLAQQSLLVGLGTILAWKYLVIGLLFLGLLN
                     TYLYLGSWSVWAYVQTTSRNLLRPLHLIPLRLGRIDLLPAVGLGLAWLAFAYAEAGIS
                     HLFGRLPL"
BASE COUNT          808 a         1272 c         1403 g          733 t
ORIGIN      
        1 ccaaagcact tgcgcagccc gcgcaccacg ttgtactcgc cgcccccttc ccagacgcga
       61 aaggcccggg cgttgcggat gcgcccctcg ccggggtcga agcggagcat gatttcaccc
      121 agagccgcca gatcgtagtg gcaggcgtcg gacggtcgga gatcgagtgt tggcatgggg
      181 gtgcgagtct gtggcggcgg tgcgtttgtc ggggtggaga atgccgcgcg cgtaggtgga
      241 agacaacgcc caagtcttga ccgggggcaa gcctgctcct ataaacgcgc cgcgatcgac
      301 cgcacaacgc attcgcaaac tctctatgaa agaagccaag tccgtttcca tcagtccgac
      361 cggcgagaag ttcccgttgc ccagtccgaa ggactacaaa aaggaattcg cgcgtctcga
      421 agctcttgcc aaggcgcggc gcaagcaggg tcaggaggtg gtcgttgtgg tcggattggg
      481 ttttgtgggg gcggtgatgg cggcagtggt ggcggatgcg cgggacaaga agagcggccg
      541 gccgaataag ctggtgattg ggatgcagcg gccgagcccg cggagttact ggaagatccc
      601 gttattgaac cgggggattt cgcccgtgaa ggccgaggat ccggaggtcg agacgctcat
      661 tgggcgctgc gtcaaggaga gcaagacgct ggttgccacc tacacggagg aggcgctccg
      721 attggccgac gtcgtggtcg tggacgtgca atgcgactat ctgaaggagt cgttgggaga
      781 tgtgcggacc ggctcggcgg acatggaggc gctcgaagcc tcgatcggca cgatcgccga
      841 acggattccc gccggcgcgc tggtcctgat cgagacgacg gtggctcccg gaacaaccga
      901 gcaggtggcc tacccgatca tgaagaaaat cttcgagcgc cgcaggatcg cgtcggagcc
      961 gctgctcgcc cacagctacg agcgcgtcat gccgggacgc aattacgtgg cgtccatccg
     1021 ggatttctgg cgcgtgtgca gcgggatcaa cgacgaagcc cgcgacaagg tcgtccggtt
     1081 cctcaaggac gtgctcaaca cggagaagtt cccgctgacc gttctggacc ggccgatcga
     1141 atccgagacc gccaagatcg ttgagaacag ctaccgggcg acgattctgg cgttcctcga
     1201 cgaatggtcg atgtttgccg agcgcaacgg ggtcgacctg aagaaggtga tcgaggcgat
     1261 ccgggttcgc ccgactcatt cgaacctgat ttttccgggg ccgggcattg gcgggtattg
     1321 tctgcccaag gacggcggcc tcggcatgtg ggcctacaag cacatcttcg gctgggaaga
     1381 caacatcttc aagatcaccc cggccgccat caacatcaac gacacccgct cgctgcacgc
     1441 gccccagttg gtgcgcgacg ccttgcgcaa catgggcaag ccgctggcgg ccgccgaggt
     1501 cctgatcctg ggggcggcgt accgggaaga cgtcggggac acgcgttaca gcggctccga
     1561 agtcatggtg cgcaagcttg ccgagatggg ggctgacgtg aaggtgcacg atccgtacgt
     1621 cgagcactgg tgggaattcg aggcccagga cacctatccg caccccggct acagtctcaa
     1681 gcggtttttc catcgccagg accagctcgt caatctgcgc atccagcccg atctcaaggc
     1741 cgccctcagc ggcgtcgacg ccgttgtctt cgcggtccgc caccagcagt acctcaagct
     1801 cgatcccgac gccgtgttcc ggcaggtggg caagccgttt gccgtcgtcg actgcttctg
     1861 catcctgagc gacgcgcaga ttcggcgcta tctcgaactc gggtgcgatg tgaagggcat
     1921 gggccgcggc cacatcaagc gcatcaaaga ctcgatctcc gcctcgaagc ggccctgaac
     1981 ccgatcggcc gggagagaga cccggctact gctcccgctg ctcgaggggg acgtagtggc
     2041 gcagccccga gccgacgtag atctggcggg ggcggtagat gcggcacttg ggatcttcca
     2101 tgatctcctt gaagttcgca atccaacccg gcatgcgccc gatcgcgaag atcacggtga
     2161 acatctcgat cggaatcccc accgctcgca tgatgatgcc gctgtaaaag tcgacgttcg
     2221 gatacagctt ccgctcgacg aagtaagagt cgttgagagc tgcttcttca agacgtttgg
     2281 cgatgtccag cagcgggtcc gcgacctgga tgcgtttgag aatctggtcg caggccttct
     2341 tgatgatcgt cgcccgggga tcgtagttct tgtagaggcg gtgcccgaag cccatcagtc
     2401 gccgcccgct gtttttgtcc ttggcggccg cgatgaattt cgagccgtca tcgccggact
     2461 tgtggatttc ctgaagcatt tcgaccacgg cctggttggc gccgccatgc agcggccccc
     2521 acagggcgca gacgcccgcc gccgcgcagg caaacaggtt ggcctgactc gacgccacca
     2581 tccgcaccgt ggccgtcgag cagttctgct cgtggtcggc atggagcagg aagatcagat
     2641 cgagcgcgcg cacaatctcc ggctgcagat tgtagtcctc gtacggatcc gagaacatca
     2701 tgtgcaggag gttggcggtg tacttgtagg cgcgcttcgg gtagatgatc ggcagccccc
     2761 gcgacttccg gtacgagaag gccgcaatgg tgcgcacctg cgagatgagc cgcgcggcat
     2821 ggatgtcgaa tcgctcggaa ttgcgcgggc cgttgagccc cggatagaaa caactgctgg
     2881 cgttgatcat cgccgagagg atcgccatcg ggtgcgccgt cgaggggaaa ccctcgaagt
     2941 ggtacttcat gtcctcatgg agattctcgt tgtcggtcag cagctcggag aacgcgcgga
     3001 attgggtgcg ggtcgggaga ttcccataga tcagaagata cgcggtctcg acgaaggtgg
     3061 atttctcggc cagctcctcg atcgggatgc cgcggtacct cagaatccca ttttccccgt
     3121 cgatgaacgt gatggcgctc cgacaggaac cggtgttcga gtatccgtcg tccagcgtga
     3181 tatgccctgt ctggtcccgc agcgcggcga tcgagaccgc aggttcgcct tcggtgcccc
     3241 tgagcgtggg cagttcgtag actcggccat cgagttccaa cttggctggc ttgatcatga
     3301 gtgttttcag gcttccaatc gcgcccgtgg gcgcaacatt cgaaccgcat tttgaaaaca
     3361 aagcgccgcc ttggcaagcg gcgaaacacc acgcgtcggt gaaaagcgga agatcacccg
     3421 accgcgccca gcttctggcg cacctggtcg accgagaagc cgcgaatgcc gacgcgtttg
     3481 tgtcgcgagg cacgcccgtg cgccagacgg agctggttgc ggggacagcc cagggtgcgg
     3541 gccaggaagc ggagcacctc ctcgttcgcc gcgtcgtcga caggcggggc ggcgatccgg
     3601 accttgagtt cgggaccgat cgctccggcc agttcgttgc gcgagctccg aggctgaacc
     3661 cggatggcga gaagaacctc gtcgccctgg aggtggagaa agtcgccggt cataacggga
     3721 gccggccgaa gaggtggctg atgccagcct cagcgtaggc gaaggccagc caggccaatc
     3781 ccagcccgac cgctgggagc aagtcgatgc gaccaaggcg caacgggatc aggtgcaggg
     3841 gcctgaggag gttgcgcgag gtggtctgaa cataggccca gacgctccaa ctgcccagat
     3901 agagatacgt gttgagcaag cccagaaaga gcaagccgat caccaggtac ttccaggcca
     3961 ggatcgttcc cagaccgacc aggaggctct gctgcgccag gtgtgccgcg gagccggggg
     4021 ccgccgtcat cccctggcgc acaagttccg catgggcaaa ccaccagagg gcgcccacca
     4081 gaaggatcgg ggccgtcagc ttcacggcga ccggccacgg ctcgagccgg ccaaggtact
     4141 gccgcaccag gcgttgccac gggtccgtgt cgggcacccg ccgattcacc atcgagagca
     4201 gcaggagcca aaggtg
//
LOCUS       DAOQRO010000310         4187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_22863_length_4187_cov_4.466360, whole genome shotgun sequence.
ACCESSION   DAOQRO010000310 DAOQRO010000000
VERSION     DAOQRO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22863_length_4187_cov_4.466360"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKK20_10915"
     CDS             complement(<1..378)
                     /locus_tag="PKK20_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNV00440.1"
                     /translation="MSGPIWFVAHTRPRCEKKLVQYCEREGFSTTLPCYRSVKKYRGK
                     KVVFYKPLFPGYVFLQLQRTERQAVYQSDYVANLLDVPDQEEFAAQLADILQALETNV
                     EVRLAPTIGPGMKVRIKSGPLQGM"
     gene            complement(375..1793)
                     /gene="argH"
                     /locus_tag="PKK20_10920"
     CDS             complement(375..1793)
                     /gene="argH"
                     /locus_tag="PKK20_10920"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNV00441.1"
                     /translation="MRKISSTAARSGRFARGPAAEVTAFSESVSFDWRLWRHDIAGSM
                     AHAAMLRRAGLLTRAELKSILGGLTAIGREIAAGTFRWRPELEDVHMNIEAELTRRVA
                     AGAKLHTGRSRNDQIALDLRLWLRDETIAMGQDLRMLQRALVALAQANVAVVMPGYTH
                     LQRAQPVFFAHHALAYVEMFDRDHDRLGDSYDRLGVCPLGSGALAGSTLPLDRAFVAR
                     QLGFVDRRGRPRLTQNSMDAVSDRDFAIEFCAICALIAVHLSRLAEDMILWASAEFGF
                     IRIGDAYTTGSSLMPQKKNPDVAELTRGKSGRVIGNLMSLLTLLKGLPLTYNRDLQED
                     KERLFDTADTVRSCLRLMAGMLSDTKVCAEACRAAAEDSQLQATDLADDLVRQGVPFR
                     EAHHLVGAAVALAEKAGRRLHELTLGEWRRISTKFGSKAPVMFDLDRAMRRRALPGAP
                     SPREVRRQLARWRRVLGNPHLP"
     gene            complement(1814..2203)
                     /locus_tag="PKK20_10925"
     CDS             complement(1814..2203)
                     /locus_tag="PKK20_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNV00442.1"
                     /translation="MDSQLSQYLPVLMLAAVAVAATAGMMILSMMLGKLGRRSKAKDT
                     AYECGMIPVGNPTPRLSMRFYLVAMLFILFDIEVIFLYPWAVVYRRFLEHPAMANPIL
                     FSMVSFLGVLLVGYLYALKKRAFEWRN"
     gene            complement(2268..>4187)
                     /gene="purL"
                     /locus_tag="PKK20_10930"
     CDS             complement(2268..>4187)
                     /gene="purL"
                     /locus_tag="PKK20_10930"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNV00443.1"
                     /translation="RDIFTMGARPVAVLGSLRFGSLANDKVKHLFKEVIRGLAHYANQ
                     AEVNAVGGETCFDESYEGNPLVNAFVVGIVEQKQIVRGTTAGQGKAVYYIGGDTGRDG
                     VGGASFASQEITEASASSSSAVAIGNPALGRRLREACLELIGKGLVAGMQDMGAAGLI
                     CSTCETASRGGTGIEFDVALIPQREPNMTPYEILLSESQERMIAIVEEGKEDDAIAIL
                     RKWSVPVVRIGCVTQDGMVRVKHNGRVVAEVPAKALTENAPLYDRPTQEPSYLGEAQH
                     LALDGLPVPADCSAVLSALLDSPTIASKEAAYRSFTFREKDTVLLGAGSDAAVVRVQG
                     TRKALAMSVDCNGRYCYLNPYQGAMLAVAEAARNIVCAGARPLAITDGLNFGNPMKPE
                     SYWQFQKCIEGLAAACRALDTPVVSGNVSFYNENPKGAVDPTPIVGMVGLIEDAETFV
                     TQEFKADGNLVALLGTPTGRLDGSEYLHLFHGLKKGNPQIDINFEKSVHNACLDAIQA
                     GLVRSAHDTSEGGLAVCLAESCISNPQQMRGAQIDLQALPTEGRRLDEILFGESPSRI
                     VVTVDPQNLPRLEAIAAKHSVPCFKLGSVGGDRLVIRHNGVKAIDLAVQAMSNTWRGS
                     IPAKAGMAPPPTALA"
BASE COUNT          778 a         1367 c         1280 g          762 t
ORIGIN      
        1 catcccttgc agcggaccgg atttgatccg caccttcatg ccgggaccga tcgtcggagc
       61 tagtcggacc tcaacgttgg tctcgagggc ctggagaata tccgccaatt gcgccgcgaa
      121 ctcttcctgg tccggcacat cgagcaggtt ggcgacgtaa tcgctctggt aaaccgcctg
      181 ccgctccgtg cgctggagct gcaagaagac gtatccggga aacagcggct tgtaaaagac
      241 caccttcttg ccccggtact ttttcacgct ccggtagcag ggcagcgtgg tggagaaacc
      301 ctcgcgctcg caatactgca ccagcttctt ctcgcaccgc ggacgcgtgt gcgccacgaa
      361 ccagatcgga ccgctcatgg aagatgcgga ttgcccaaca cccgacgcca ccgcgccaat
      421 tgccgccgga cttcgcgcgg gctgggcgcc ccgggcagcg cgcgccgtcg catcgcacgg
      481 tccagatcga acatcacagg ggccttcgat ccgaatttcg tcgaaatccg gcgccattcc
      541 ccgagcgtca gttcgtgaag ccgcctgccc gccttctccg cgagagccac ggccgctccc
      601 accaggtgat gcgcctcgcg gaacggcacg ccttgacgca ccaggtcatc ggccaaatcg
      661 gtcgcctgaa gctgcgaatc ctccgccgcg gcgcggcacg cctcggcgca gaccttcgtg
      721 tccgacagca tgccagccat cagtcgcaga cagctccgga ccgtgtccgc cgtgtcgaac
      781 aaccgctcct tatcctcctg caaatcccga ttgtacgtca aaggcagtcc tttcaggagc
      841 gtcagaaggg acatcagatt tccaatcacc cgcccgctct tgccgcgcgt cagctcggcc
      901 acatccgggt ttttcttctg tggcataagc gacgatccgg tggtgtaggc gtcgccgatg
      961 cggataaacc caaactcggc gctcgcccac aggatcatgt cctccgccaa ccggctcaag
     1021 tgaacggcaa tcagcgcgca aatcgcgcaa aactcgatcg caaaatcgcg atcgctcacc
     1081 gcatccatcg agttctgcgt cagccgcgga cgcccgcggc gatcgacgaa tccgagctgc
     1141 cgggccacga atgcccggtc gaggggcagg gtgctgcccg ccaaggcccc gcttcccagc
     1201 ggacacaccc ccagccggtc gtaactgtct cccaaccggt catgatcccg atcgaacatc
     1261 tcgacgtaag ccagcgcgtg atgcgcgaag aagaccggct gcgcgcgctg cagatgcgtg
     1321 tagccgggca tgacaactgc aacattcgcc tgggccaggg caacaagcgc cctctgcaac
     1381 atgcgtagat cctgtcccat ggcgatcgtc tcgtcacgca gccagagccg caggtccagc
     1441 gcgatctggt cgtttcgcga ccgtccggtg tgcagctttg cccccgccgc cacccggcgt
     1501 gtcagctccg cctcgatgtt catatgcaca tcctcgagtt ccgggcgcca gcggaatgtg
     1561 cccgcggcga tctcgcgtcc gatcgccgtc aagccgccca gaatggactt cagttcagcc
     1621 cgggtcagca gccctgcgcg acgcaacatc gccgcatgcg ccatcgaccc ggcaatgtca
     1681 tgccgccaaa gtcgccaatc gaaggaaacg gattccgaaa acgcggtcac ctcggccgcc
     1741 ggcccgcgtg cgaaccggcc cgaccgggct gccgtcgacg atatcttcct catgaacgaa
     1801 agcggaatgt cggctagttg cgccactcga acgcccgttt cttcaaggcg tagagatagc
     1861 ccaccaggag cacgccgaga aacgagacca tcgagaagag aatcggattt gccatggccg
     1921 gatgctcgag gaatcggcgg taaacgaccg cccatggata gagaaaaatc acctcgatat
     1981 cgaataggat gaacagcatc gccacgaggt agaaccgcat cgagagccgc ggagtcggat
     2041 tcccgacggg gatcatgccg cattcgtagg cggtgtcttt ggccttggac cgcctcccca
     2101 gtttgcccag catcatcgac aagatcatca tgcctgcagt ggctgcgaca gccacggcgg
     2161 ccagcatcaa gaccggcagg tactgggaca actgcgaatc catgtgccga cgctaaaaag
     2221 accctcgcgg gttgacaagg ggaaacggac gcgcaggcgc taattcgcta cgcgagagcc
     2281 gttggcggag gcgccatgcc ggctttcgcg gggatcgagc cgcgccaggt gttgctcatg
     2341 gcctggacag cgagatcgat cgccttcacg ccgttgtgcc ggatcacaag gcggtctccg
     2401 ccgactgaac cgagcttgaa acagggcacg gagtgtttgg cggcgattgc ctcgagtcgg
     2461 gggagattct ggggatcgac ggtgacgaca atccgcgacg gactctcgcc aaacaggatt
     2521 tcatcgagtc gcctgccctc tgtcggcagg gcttgcaggt cgatctgcgc gccgcgcatc
     2581 tgttgcggat tgctgatgca cgattcagcc aggcagacgg cgagaccgcc ttctgacgtg
     2641 tcgtgggcgc tccggaccag tccggcctgg atggcatcga ggcaggcgtt gtgaaccgac
     2701 ttctcgaagt tgatgtcgat ttggggattg cccttcttga ggccgtggaa gaggtggaga
     2761 tactcgctgc catccagcct gccggtgggt gtgcccaaca gcgcgacgag gttgccgtcc
     2821 gccttgaatt cctgtgtgac gaaggtttcg gcgtcttcga tcaaaccgac cattccaacg
     2881 atcggcgtgg ggtcgacggc gcctttggga ttctcgttat agaagctcac attgccgctg
     2941 accacgggcg tgtcgagagc gcgacaagcc gcggccagcc cttcaatgca tttctggaat
     3001 tgccagtagc tttccggctt catcgggttg ccgaagttca ggccgtcggt gatggcaagc
     3061 ggtcgggcgc cggcgcagac gatattgcgt gccgcttcag ccacggcgag catggcgccc
     3121 tgataggggt tgaggtagca atagcggcca ttgcagtcca cgctcattgc cagggccttg
     3181 cgcgtgcctt ggacccggac caccgcggcg tccgacccgg cgcccagcag gacggtgtcc
     3241 ttttctcgga aggtgaagct gcgataggcg gcttccttgc tggcgatggt cggcgagtca
     3301 aggagcgcac tcagcacggc gctgcaatcg gcgggcaccg ggagcccgtc gagcgccagg
     3361 tgttgcgcct cgcccaaata ggatggttcc tgggtcgggc gatcgtagag cggcgcgttt
     3421 tcggtgaggg ccttggccgg cacctcagca accactctgc cgttgtgttt gactcggacc
     3481 atgccgtcct gggtgacgca cccgatccgc acaacgggga cactccattt cctgaggatc
     3541 gcgatcgcgt cgtcttcctt gccttcctcg acaattgcaa tcatgcgctc ctgcgattcg
     3601 gaaagaagaa tttcgtaggg cgtcatgttc ggttcgcgtt gggggatcaa cgcaacgtcg
     3661 aactcgattc cggtgccgcc gcgcgaggcc gtctcacacg tcgagcagat gagtcccgcg
     3721 gctcccatgt cctgcatgcc ggccaccagc cccttcccga tcagttcgag gcaggcttcc
     3781 cgcaggcgcc ggcccagagc cggattgccg atggcgaccg cgctcgacga ggaggcggat
     3841 gcctcggtga tttcctggga agcaaagctc gcgcccccga cgccgtcgcg tcctgtgtct
     3901 ccgccgatgt agtaaaccgc tttgccctgg ccggcggtgg tcccgcgcac gatctgtttc
     3961 tgctcgacaa tcccgacgac gaacgcgttg acgagcggat tgccctcgta gctttcgtcg
     4021 aagcaggtct cgccgccgac ggcgttgacc tcggcctggt tcgcataatg ggcaagtccg
     4081 cggatgactt ccttgaacag atgcttcacc ttgtcattgg ccagactgcc gaagcgcagg
     4141 gagcccagga cggcgacggg cctcgcgccc atcgtgaaga tgtcccg
//
LOCUS       DAOQRO010000311         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_23009_length_4170_cov_4.850790, whole genome shotgun sequence.
ACCESSION   DAOQRO010000311 DAOQRO010000000
VERSION     DAOQRO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23009_length_4170_cov_4.850790"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKK20_10935"
     CDS             <1..578
                     /locus_tag="PKK20_10935"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNV00444.1"
                     /translation="MSGWNRTRTWKATRLPWVPTSPNVKSAETGLFLVATGVLGEIGG
                     VSIGMGTDMPFQCVAAPWLKSDALASHFAGLRLRGVQFSPVSYTPSRGMFQGQRVSGV
                     RIRFSEPDQAPLVAINYYALEAVRQVAGRDLFALALKRGQSFQMLDRVNGTDSVRRAL
                     QEGRSARSIVASWKAGEDTFRKKRAPYLLYR"
     gene            complement(644..1279)
                     /locus_tag="PKK20_10940"
     CDS             complement(644..1279)
                     /locus_tag="PKK20_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine esterase"
                     /protein_id="HNV00445.1"
                     /translation="MLSTDLMPAREERSRRLMIVLHGLGDSMEGYRWLPPALNLPWLN
                     FLLVNAPDDYFGGYSWYDIYGDAEPGIVRSRTLLFDLLDSLGGRGFAVADTVLFGFSQ
                     GCLLTIDVGMRYPQRLAGLVGISGYAHDLDSLLQEMSPLAKQQRFLVTHGTQDPLLPI
                     EPVRGQMQRLRRAGLKLEWREFAKAHTIAGEAEIDVIRTFIENCFASNAGA"
     gene            1394..2377
                     /locus_tag="PKK20_10945"
     CDS             1394..2377
                     /locus_tag="PKK20_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00446.1"
                     /translation="MRRTSVFLLLLAMAGLFWGPAWAADYKLLDGSTISGEISDGNDY
                     GVAFRLDIGGFSKRISYSKFTQEALKLLAENPKLKPMVEPFIELPPEFTKPKPKPTIR
                     LRDVPRADRPPGRTTFFSSFTTPLGLVILGVLYLANLLAAYEIARYRNRPVALACGLS
                     ALLPVLGPIIFLVSPSLEPAEESEATAPEEALGGPAIPQASSPAGATSRKVGVPAPLA
                     AGGLRMAAAAKTEERTEAKAYKRGDYTFNRRFFETQFSGFFRVVPTDADKDMVLVVTT
                     AKAEYVGKRISRISAADFFLQLLMPAGKEVGISFAEVAQIAVKHKDEIRTR"
     gene            2826..3809
                     /gene="fmt"
                     /locus_tag="PKK20_10950"
     CDS             2826..3809
                     /gene="fmt"
                     /locus_tag="PKK20_10950"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNV00447.1"
                     /translation="MHPMRIVFFGTAELACASLEALAATPAFSLLGVVTQPDRPRGRD
                     LRLQCSPVKASALKLGLPVLQPEKCRHSDFIQHIQSLRPDVGVVVAYGQILPPALLGI
                     PPHGCVNVHASLLPKYRGAAPIQWAILNDESETGVTIMQIDAGLDTGPILAMEATPIR
                     PDDNARTLHDRLARSGADLLVRTLSEQVSGRLVPRPQPEEAATYARKITKEDGRLDWN
                     RPARELWNRVRGLAPWPGTYTHLRSDPKPMLLKVHEASVRPGPRGAAGEILAADRAGI
                     VVGCGEGALCLTAVQREGGRRLTAAEFLAGHPLRPGQCFQNLEVSTREPAG"
     gene            complement(3806..>4170)
                     /locus_tag="PKK20_10955"
     CDS             complement(3806..>4170)
                     /locus_tag="PKK20_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HNV00448.1"
                     /translation="NGVLVMNTFGDFLSGRDFLLASIEQTLKSVFPSTRIHAAGSGNV
                     FFVASPQAGLEVRRTPDFSSLPPDLRWQAEQAFGSKPATDPAHGMVLTDDFNPADYRD
                     AFNREDLRRRLAMSYRPD"
BASE COUNT          731 a         1348 c         1324 g          767 t
ORIGIN      
        1 cgatgtccgg ctggaaccgc acgaggacgt ggaaagcgac acgcctgcct tgggtgccca
       61 cctctccgaa tgtgaagtcg gccgagaccg gcctgttcct ggtcgccacg ggcgtgctgg
      121 gtgaaatcgg cggcgtgagc atcgggatgg ggaccgacat gcctttccaa tgcgttgcgg
      181 cgccgtggct caaatccgat gccctggcgt ctcacttcgc gggtctgcgg ctgcgcggag
      241 tgcagttctc gcccgtcagt tacacgccgt cgcgcggcat gttccagggc cagcgtgtct
      301 cgggcgtgcg gattcgattc agcgagccgg accaggcccc gctggtggcg atcaattact
      361 acgcgctcga agcggtccgg caagtggcgg ggcgggacct gttcgctctg gcgctcaaac
      421 gcggccagtc gtttcagatg ctcgacaggg tcaacggcac cgattccgtt cggcgcgcgc
      481 tgcaggaggg ccgcagcgcc cgcagcattg tggcgtcgtg gaaagccggt gaagacacgt
      541 tccgcaagaa gcgcgcaccc tacttgcttt atcggtaacc gggggccgcg gtgccgacta
      601 gccggcggcg cggcgctctt gacctgcgaa gcgctgccgg aggtcaagcg ccggcgttcg
      661 aggcaaagca gttctcgatg aacgtccgga ttacgtcgat ctcggcctcg ccggcgatgg
      721 tgtgggcttt cgcgaactca cgccattcga gcttcagccc agcgcggcgc agccgctgca
      781 tctggccccg gaccggctcg atgggaagga gtggatcctg ggtcccgtgt gtcaccagga
      841 agcgctgctg tttggccaaa ggcgacattt cttgcagaag cgagtcgaga tcgtgcgcgt
      901 agccgctgat gcccaccaac ccggccagcc tttgcgggta gcgcatgccc acatcgatcg
      961 tgagcaggca accctgggaa aaaccgaata ggacggtgtc ggcgactgca aagccccgtc
     1021 cgcccagact gtcaagcaga tcgaacagga gcgttcggct tcggacgatt cccggctccg
     1081 catcgccgta gatgtcgtac catgagtatc cgccgaaata gtcgtcgggc gcgttgacca
     1141 ggaggaaatt gagccagggc agattcagag cgggcggaag ccagcgatac ccttccatgc
     1201 tgtcgcccaa tccatgcaac acaatcatca gtcggcgcga tcgttcctcg cgggccggca
     1261 tcaagtccgt actcagcatt tgcggattca accgggaacg cgccgccaag accagcctgg
     1321 aatcgtccgg gcgggaggac gccgtgtgaa tttcttcctt ggccgcgggt gaaatgtcgt
     1381 tcagtctccg gacatgcgta ggacatcagt tttcctgctg ttactggcta tggccgggct
     1441 gttctgggga ccggcctggg cggcggatta caagcttttg gacggaagca cgatcagcgg
     1501 cgaaatctcc gacggcaacg attacggagt cgctttccgg ctcgacatcg gcgggttctc
     1561 gaagcgcatt tcttatagca agttcacgca ggaggccttg aagctgctgg cggagaatcc
     1621 gaaactcaaa ccgatggtcg agccgttcat cgaattgcct ccggagttca ccaagcccaa
     1681 acccaagccg acgatccgcc tgcgggacgt gccaagggcc gaccgtccgc cgggacgcac
     1741 cacgttcttc agttcgttta ccacgccgtt gggactcgtc attctgggtg ttctctacct
     1801 ggccaatctg ctggctgcct atgagatcgc ccgctatcgc aaccgcccgg tcgccctggc
     1861 ctgtggattg tccgcgttgc tgcccgtgtt ggggccgatc atcttcctgg tttcgccgtc
     1921 cctggaacct gcggaagagt cggaggccac cgcgcccgag gaggcattgg gaggaccggc
     1981 gataccccaa gccagcagcc ccgcgggtgc cacctcccgc aaagtgggtg tgccggctcc
     2041 gctcgccgcc gggggtctcc ggatggcagc cgccgcaaag acggaggaga ggaccgaggc
     2101 caaggcctac aagcgtggcg attacacgtt caatcgccgc ttcttcgaaa cccagttctc
     2161 cggcttcttc cgcgtggtgc cgactgacgc cgacaaggac atggtgctgg tggtgaccac
     2221 agccaaggcc gagtacgtgg gcaagcgcat cagccgcatc tccgcggcgg atttcttcct
     2281 ccaattgctg atgccggccg gcaaggaggt gggcatttcg ttcgccgaag tggcccagat
     2341 cgccgtcaaa cataaagacg aaatccgcac ccgatgaggc tctgccgcat tcgtctgctc
     2401 gacacaccgc cgcgcggcac cgatgatctc cgcggcgttt cggcctttag agtccttgac
     2461 tccttcaacc ctctccatgg acttgacttg tccacatcag gcctcactgg acaacgattc
     2521 tagcgcccat gactcagcag ttcggcgatt tcaccgccag cgaattgtac tgccccaagt
     2581 gccgccgggc gatgccggtg cggcagaagc tcctgctcat tctcccgagc ggcgaactct
     2641 acgaatacct ctgttccggc tgcgccacat ccctgggtga aaggacggtc tcgagtccaa
     2701 gcgtgcgtgc cgccagcccg aggcctcccg cccgacgttc gcgttcgccc cgtgggtccg
     2761 gcccaacccc gcgatcgata cgcggattgt tgcgttagcg agacgcgcct cagaccatcc
     2821 ctccgatgca tcccatgcgc atcgtcttct tcggcaccgc cgagctcgcc tgcgccagcc
     2881 tggaagccct cgcggcaacg cccgctttca gcttgttggg ggttgtgacg cagccggatc
     2941 ggccccgtgg gcgtgatctc aggcttcaat gctcgcccgt caaagcgagt gcgctcaaat
     3001 tgggtctgcc ggtcttgcag cccgagaagt gccgtcactc ggacttcatc caacacatcc
     3061 aatcgctccg gcccgatgtc ggtgtggtcg tcgcttacgg tcaaatactc cctcccgcgc
     3121 tcctgggcat cccccctcac gggtgcgtga acgtgcacgc ctcgctgctg cccaagtacc
     3181 gcggggccgc ccccatccag tgggcgatcc tcaacgacga atcagagacc ggagtgacca
     3241 tcatgcagat cgacgccggg ctcgacaccg ggcctattct tgcgatggag gccacgccga
     3301 ttcgacccga cgacaatgcg cggactctgc acgaccgtct ggctcgatcc ggcgccgact
     3361 tgctggtccg cactttatcc gagcaggttt cggggcgcct cgtgccgcgc ccccaaccgg
     3421 aggaagccgc cacgtacgcc cgcaagatca caaaggagga tgggcgtctg gactggaacc
     3481 gcccggcgcg cgaactctgg aaccgcgttc gcggacttgc gccgtggccg gggacgtaca
     3541 cccatctgcg gagcgatccc aagccaatgc tgctgaaggt tcatgaggca tcggtgaggc
     3601 ccgggcctcg aggcgcggca ggcgaaatcc tcgctgccga tcgagcgggc atcgtggtgg
     3661 ggtgcggcga gggcgctctc tgcctgacgg cggtgcagcg cgagggtggg cgccggctca
     3721 cggcggccga atttctcgcc gggcatcctc tgcgtcccgg gcagtgtttc caaaacctcg
     3781 aagtctcgac ccgtgaacct gcaggctagt cgggccggta actcatcgcc aacctccggc
     3841 gcaaatcttc ccggttgaag gcatcccgat agtcggctgg gttgaagtcg tctgtgagca
     3901 ccattccgtg ggcggggtcc gtcgccggtt tggacccaaa ggcctgttct gcctgccagc
     3961 gcagatcagg cgggagggat gagaaatcgg gtgtgcggcg cacctcgagg cctgcctggg
     4021 gagaggcgac aaagaacacg tttccgctgc ctgcggcatg gatgcgtgtg cttggaaaga
     4081 cgctcttgag cgtctgctcg atcgaggcaa ggagaaagtc gcgccccgag agaaagtcgc
     4141 cgaaggtgtt catcaccagg acgccgttgg
//
LOCUS       DAOQRO010000312         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_23152_length_4154_cov_4.098073, whole genome shotgun sequence.
ACCESSION   DAOQRO010000312 DAOQRO010000000
VERSION     DAOQRO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23152_length_4154_cov_4.098073"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKK20_10960"
     CDS             complement(<1..499)
                     /locus_tag="PKK20_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV00449.1"
                     /translation="MSRSFGNAVRDFTASFASLARLPRAFWIVIGAFVVESSAYFGVL
                     TLMASYVSRDLGWSDVRAGFTVSLFTMAVTLFMLGAGSYAEGFGLRRAILFALLICTG
                     GRALYSLAPVWSSTIAGGAVLVSLLAVAVGAGILQPVCYSGVKQYTDQRTSSMGYALI
                     YAIMNL"
     gene            complement(560..1453)
                     /locus_tag="PKK20_10965"
     CDS             complement(560..1453)
                     /locus_tag="PKK20_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNV00450.1"
                     /translation="MLPSDRKAACPHSLWFRGADAGPARVAAFTLIELLVVIAVIAIL
                     AALLLPALTAAKQAGLSARCQGNLRQLGLSLLMYTEDYSRFPVYNFDPDAFEPMEFWH
                     EKLTPYVQARWTNEIYRCPAYRGATVDGNDVATPLGSYGYNANGTQWSYSELGLGGRY
                     SKMVWEGDSPDGNTSLVAITQSTVKVPSDMIALGDAHLIWVNPLLLRILYDLKSQETY
                     SGMALIDINSRNNVQAPKWPGSAGVLKATRQRHRNRSNVLFCDGHVQNIREEKLFERT
                     DSALRRWNNDNEPHAEKLINL"
     gene            complement(1463..2566)
                     /locus_tag="PKK20_10970"
     CDS             complement(1463..2566)
                     /locus_tag="PKK20_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00451.1"
                     /translation="MNTRSSFETLSVVALLALLAPQTTRAQVQIKASDMFNQPGQYYR
                     AYAGSSDVDVSGRLGGTGGPQAWDFTSGPQGITLRFDYIAIDQADHACDFPGATFAER
                     KTNESDGSTAWMYLEQATGRGRMNYGFYDQAFSDTLPSCPFTLPIRDFPESIGYGDGW
                     SVATVFLSELRIPDIGDDEGGGEDFVIPLQITYSASAKADAYGIVNQPGIDFGECIRV
                     NELAQWDIAVDLGLGDGFQTISTQYTRNYYWLRPGRGIVVQITSKQLDTPPLDEFPVA
                     AEFIRMFETNHPDSVPDKPSIKDFKITVSKDGALLTWTKGSGLTRYQVDVTDNPGLPG
                     SWTTLATTTWNYHIDSAANKPETPRRFYRVVGH"
     gene            3150..>4154
                     /gene="gap"
                     /locus_tag="PKK20_10975"
     CDS             3150..>4154
                     /gene="gap"
                     /locus_tag="PKK20_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNV00452.1"
                     /translation="MAVKVAINGFGRIGRLVYRAMVEQGLVGKDVQVVAVGDIVPAGN
                     LGYLLKYDSVQGRCAATISSEKSSPDKPDDDVLVVNGQKIQVVSAKDPSQLPWKAMGV
                     DIVIESTGLFTNVEMAKGHLAAGAKKVIITAPAKGDCPTLVIGVNENVYNPTQHHVVS
                     NASCTTNCLAPIVYVLLKEGFGVEEGLMTTIHSYTATQKTVDGPSKKDWKGGRTAAIN
                     LIPSTTGAAKAVGLVLPEVKGKLTGMAVRVPTPTVSVVDLTVRTVKETSYAEISAAMK
                     RASETYLKGILEYTEDEVVSTDFIHCPASSIYDKGSGIELNKRFFKLVSWYDNEWGYS
                     C"
BASE COUNT          849 a         1241 c         1272 g          792 t
ORIGIN      
        1 ccaggttcat gatcgcatag atcagcgcgt agcccatcga actcgttctc tggtcggtgt
       61 attgcttcac gccggaatag cagaccggtt gcagaatgcc cgccccgacc gcaaccgcca
      121 gcaacgacac aagcacggca ccgcccgcga tggtgctgct ccagaccggc gccagactgt
      181 agagcgcccg gcctcccgtg cagatcaaca gggcaaacag aatcgcccgc cgcagtccga
      241 acccttcggc gtaactgccc gcgccaagca tgaagagcgt gacggccatc gtgaacaggc
      301 tcacggtgaa ccccgcccgc acatcgctcc acccaaggtc cctcgagaca taagacgcca
      361 tcagggtcag aactccgaag tacgcgctcg actcgacgac aaacgccccg atgacaatcc
      421 aaaaagcgcg tggtaaacgg gccaggcttg cgaacgacgc cgtgaagtcg cgcactgcat
      481 tcccgaagga ccggctcata caggacaggc ggccccagcg gctgcagatg tgattctcgt
      541 ggtcacgggg accgacagcc taaaggttga tgagtttctc ggcgtggggt tcgttgtcgt
      601 tgttccagcg gcgcagggcg gaatccgtgc gctcgaagag cttctcctcc cggatgttct
      661 ggacgtgccc gtcgcagaac aggacattcg accggttgcg atggcgctgc cgagtcgcct
      721 tgagaacacc ggcactgccc ggccacttcg gagcctgaac gttgttgcgc gaattgatgt
      781 cgatcaaggc catgccgctg tacgtctcct ggctcttgag gtcatacaga atgcgcagca
      841 ggagcgggtt cacccaaatc agatgcgcat cgcccagcgc gatcatgtcg ctggggacct
      901 tgacggtcga ttgggtgatg gcgactagcg acgtgttgcc gtcgggcgaa tcgccttccc
      961 agaccatttt cgagtagcgt ccgccgagtc ccagctcgct gtagctccat tgggtcccgt
     1021 tggcgttgta gccgtaactg cccaagggcg tggccacgtc gtttccatcc accgtcgcac
     1081 cccgataggc cgggcaccgg tagatctcgt tggtccaccg ggcctggaca taaggcgtga
     1141 gtttctcgtg ccagaactcc atcggctcga aggcgtcggg atcgaagttg taaacgggga
     1201 aacgcgagta gtcctccgtg tacatcagca gcgagaggcc gagttggcga agattgccct
     1261 ggcagcgcgc gctcaggccg gcttgcttgg cggcggtcaa ggccggcaac aggagggcgg
     1321 ccaggatcgc aatgacggca atgacgacca gaagttcgat cagcgtaaag gccgcaacgc
     1381 gcgccggacc tgcgtccgcc ccgcggaacc acagggagtg agggcaagcc gctttgcggt
     1441 cgctgggtag cataggtggc gtctagtgac ccacgacccg atagaagcgg cggggcgttt
     1501 cgggcttgtt ggccgcgctg tcgatgtggt aattccaggt cgtcgttgcc agtgtggtcc
     1561 acgagccagg caggcccgga ttgtcggtca catccacttg gtatctggtc aggccgctgc
     1621 ctttggtcca ggtcaacaac gccccgtcct tgctgacggt gatcttgaaa tccttgatgc
     1681 tcggcttgtc gggcacgctg tcagggtggt tcgtctcgaa catgcgaatg aactcggccg
     1741 ccacgggaaa ctcatcgagc ggcggtgtgt cgagctgctt ggatgtgatt tgaaccacaa
     1801 tcccacggcc cgggcgaagc cagtaataat tgcgggtgta ctgggtcgag atcgtctgga
     1861 atccgtctcc cagaccgagg tcaaccgcaa tgtcccattg cgcaagctcg ttgacgcgga
     1921 tgcattctcc aaagtcgatt ccgggctggt tgacgatccc gtaggcatcc gcttttgccg
     1981 acgcgctgta agtgatttgc agcggaatca caaagtcctc gccgccccct tcgtcatccc
     2041 cgatatccgg aatcctgagt tcggaaagaa agacggtcgc caccgaccag ccgtcgccgt
     2101 agccgatcga ctcggggaaa tcccgaatgg gcagtgtgaa tgggcatgag gggagagtgt
     2161 cgctgaacgc ctggtcgtag aagccgtagt tcatccgccc acgcccggtc gcttgctcaa
     2221 ggtacatcca cgccgtcgag ccgtcgcttt cgttcgtctt cctctcggca aaggtggcgc
     2281 ccggaaaatc gcaggcgtgg tcggcttgat cgatcgcaat gtagtcgaag cggagcgtga
     2341 tgccttgcgg tccgctcgtg aaatcccacg cctggggtcc gccagtcccg cccagacgcc
     2401 cggaaacgtc gacgtccgag ctcccggcat aagccctgta gtattgaccg ggctgattga
     2461 acatatcgct ggctttgatc tgaacctggg cacgcgtcgt ttgcggcgcc aacagagcca
     2521 gcaacgccac cacgctcagg gtctcgaaag aagaacgagt gttcatgatc gggtttcttt
     2581 tcccctgcat tctgacacaa gcggagacaa aattccactt tgttcctggt tatcaccggc
     2641 atcccacgtc cgccggcgat cttggggcgc gcccagggtc aacacgcccg ctgaatccga
     2701 gtccaagctc aatccgtgcc agattccgag tcaggcagcc tggcgatcga gagcgaaaga
     2761 cacaacggac acctcgtcgc gatcaggtcc acacgccgcg tccgcccgcg tgttgcgccc
     2821 gaccatgcgc ttgatgcact ggcacttgga tattgattgc gggagaatgc tcacggagca
     2881 gggcgccttt caagtggaat cggggtcgat tgcgacaatc cgacaatctt ccggcgcaag
     2941 tcggaggtgg cggcaacctc catccggtgt ggagtgggga cgacctcgtc cccggtgatg
     3001 agggttggag accctgtttg gcgggcgacg aggggggggc cagtgtcgcc gccctgaatt
     3061 ccgttgacgg ttgggcggtg cccagtaaca tccggtcttt gattatttgg cgagtgatta
     3121 accacatctg aaaacgaaag aaaacgtgta tggcagtgaa agtcgcaatc aacggtttcg
     3181 ggcggatcgg tcgcttggtg taccgcgcca tggtcgaaca aggtcttgtt ggcaaagacg
     3241 ttcaagtcgt tgcagtgggc gatattgtcc ccgcaggcaa cctcggctac ttgctcaaat
     3301 acgattccgt ccagggtcgc tgcgcggcga ccatttcatc ggaaaaatcc tccccggaca
     3361 agccggacga tgatgtgctg gtggtcaacg gacagaagat tcaggtggtg agcgcgaagg
     3421 acccgtccca gttgccttgg aaagcaatgg gagtggacat cgtcatcgag tccaccggcc
     3481 tgttcacaaa cgtcgagatg gccaaaggcc acctcgccgc gggagccaag aaggtgatca
     3541 tcacggcccc cgccaagggc gattgcccca ccctcgtcat aggcgtgaac gaaaatgtct
     3601 acaaccccac ccagcaccat gttgtgtcga acgcttcctg cacgaccaac tgcctcgctc
     3661 cgattgtcta tgtcctcctc aaggagggat tcggagtcga ggaaggtctg atgacgacga
     3721 ttcactccta caccgcgacc cagaagaccg tcgatggacc gagcaagaag gactggaaag
     3781 gcgggcgtac cgcggccatc aacctcatcc cctcaaccac cggcgcggcc aaggcggtcg
     3841 gattggtgct gccggaagtc aagggcaagc tcaccggcat ggccgtccgg gtcccgaccc
     3901 cgactgtctc ggtcgtggat ttgacggttc gcacggtgaa ggaaacgagc tatgctgaga
     3961 tcagcgccgc catgaagcgt gctagcgaga cgtatttgaa gggcatcctc gagtacacgg
     4021 aggatgaggt cgtgagcacg gatttcatcc attgcccggc gtcctcgatc tacgacaagg
     4081 gatcgggcat cgaactcaac aagcgtttct tcaaactcgt gagttggtac gacaacgagt
     4141 ggggctatag ctgc
//
LOCUS       DAOQRO010000313         4087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_23695_length_4087_cov_4.092758, whole genome shotgun sequence.
ACCESSION   DAOQRO010000313 DAOQRO010000000
VERSION     DAOQRO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23695_length_4087_cov_4.092758"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="rpoB"
                     /locus_tag="PKK20_10980"
     CDS             <1..1172
                     /gene="rpoB"
                     /locus_tag="PKK20_10980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463141.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNV00453.1"
                     /translation="VGEAKKQARQVEEEHRQKTEELREQLTEALSNILLGEKIPLDVV
                     NSETGEIIIPANRKITKTLLRKLAQVYDRIEIDPSPIRNKINEIIGGFKKKFDDLQMQ
                     FDEEMERVEAGDELDTGIIKSVKVYIASKRKLSVGDKMAGRHGNKGVVARIVAEEDMP
                     FLADGRAVDIVLNPLGVPSRMNVGQVLETHLGWAAHQLGLRFSTPVFDGIKEKDIRTY
                     LKQAAAKQREAAEGLLVAENGKSILYDGLTGEPFDQEIVVGYIYMMKLGHLVADKIHA
                     RAVGPYSLVTQQPLGGKAQYGGQRFGEMEVWAMEAYGAAYTLQELLTVKSDDVQGRTR
                     IYESIVKGDNALEAGIPESFTVLVKEMQSLGLDVKVGYTNPVDQAAQPTALGVLG"
     gene            1212..>4087
                     /gene="rpoC"
                     /locus_tag="PKK20_10985"
     CDS             1212..>4087
                     /gene="rpoC"
                     /locus_tag="PKK20_10985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV00454.1"
                     /translation="MLSTKESARKLLGLEKVNLVDYVAISVASPETIRSWSKGEVKNP
                     ETINYRTFKPEKGGLFCERIFGPVKDWECSCGKYKRIKHRGVVCDRCGVEVTLSRVRR
                     ERMGHIDLAVPVSHIWFFKCMPSRIGLMLDMTARNLERVIYYEDYLVIDGGDTPLVRH
                     QLLSEMEYREARETYGPEAFTAKMGAEAVRDALQNIELQKTIDELQQAMTETRSKQIR
                     KKLAKRIKLFQGFLTSRTRPEWMILTVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRV
                     INRNNRLKNLLQLKTPEVIIRNEKRMLQEAVDALFDNGRHGRAVTGAGNRALKSLSDM
                     LKGKSGRFRQNLLGKRVDYSGRSVIVIGPELKLHQCGLPKKMALVLFEPFIIRRLKEL
                     GYVHTVRSAKKMIERQSPEVWDILEEVTKGHPVLLNRAPTLHRLSIQAFEPVLIEGEA
                     IRVHPLVCTAYNADFDGDQMAVHVPLSVEAQLETRLLMMAPLNVFSPSSGRPIMTPTQ
                     DITLGCYYLTAEPRTPRKPRQRLMLFGSKAEALTAHVDGALGMHDRVLLANPDRGRQT
                     TYGEPDRSTIETTVGRIIFSEIWPPELGFFNKVAGKAQLGQIIWNCYKLCGHERTIVA
                     LDRLKEVGFREATGSGCSIGIDDMIIPKEKDQEIAAAQRQIGEVEKQYRRGIITPGER
                     YNKIIDIWTHCTDQISNVMIQTLESNQGKREYNPVWLMVDSGARGNKAQVRQLAGVRG
                     LMAKPDGSIIEKPIVSNFREGLTVLEYFISTHGARKGLADTALKTADSGYMTRKLVDV
                     AQDVIIREEDCGTTNGIWVHAIYEGEDEVVKLTDRIVGRVACDDIPDPRDPKHKLVQA
                     NQEIDEQIAREIEASDLERVRIRSVLTCESKKGVCAACYGRNLATGGKIKIGEAVGII
                     AAQSIGEPGTQLTMRTFHIGGTASQVFKQPQIKAKYDGIVRYNDL"
BASE COUNT          865 a         1173 c         1331 g          718 t
ORIGIN      
        1 ccgtgggcga ggcgaagaag caggcgcggc aggtcgagga ggagcatcgt cagaagaccg
       61 aggagttgcg ggagcaactg acggaggcgt tgagcaacat cctgctgggc gagaagattc
      121 cgctggacgt ggtgaactcg gagacgggtg agatcatcat tccggccaac cggaagatca
      181 caaagaccct tctgcgcaag ctggcgcagg tctacgatcg catcgagatc gatccgtccc
      241 cgatccggaa caagatcaac gagatcatag gcggcttcaa aaagaagttc gacgatctcc
      301 agatgcagtt cgacgaggag atggagcgcg tcgaggccgg cgacgaattg gacaccggga
      361 tcatcaaatc ggtcaaggtc tatatcgctt cgaagcgcaa actctccgtg ggcgacaaga
      421 tggccgggcg gcacggaaac aagggcgtgg tggccaggat tgttgcggag gaggacatgc
      481 cgtttctggc ggatgggcgg gcggtggaca ttgtcctgaa tccgctgggt gtgccctcgc
      541 ggatgaatgt ggggcaggtt ctcgagacgc atctgggctg ggcggcgcat cagttgggtc
      601 tgaggttctc gacaccggtt tttgacggca taaaggagaa ggatatccgg acctacctca
      661 agcaggccgc ggccaagcag cgcgaggcgg cggagggttt gctggtggct gagaacggca
      721 agtcgattct ctacgacggt ctgacgggag agccgttcga tcaggaaatc gtggtgggtt
      781 acatttacat gatgaagctt ggccatctgg tggccgacaa gattcacgcg cgggccgtgg
      841 ggccgtactc gcttgtgacg cagcagccgc tgggcggcaa ggcgcagtat ggcggacaac
      901 ggttcggcga gatggaggtc tgggccatgg aggcctacgg ggccgcgtac acgcttcagg
      961 agttgctgac ggtgaaatcc gacgatgtcc aggggcgcac gcggatctat gagagtattg
     1021 tcaagggcga caacgccctg gaagccggca ttccggagtc gttcaccgtt ctggtaaagg
     1081 agatgcagtc tctggggctg gacgtgaagg tcggttatac gaatccggtg gatcaggccg
     1141 cccaacccac ggccctggga gtgctgggtt gaggagcgat gatcggttca agcctttgac
     1201 cccatttgtc tatgttgagc accaaagagt cagcacgcaa gctgttgggc ctcgagaagg
     1261 tcaacctggt agattacgtg gccatcagcg tggcttcgcc cgagaccatt cgttcctggt
     1321 ccaagggcga agtcaagaat cccgaaacca tcaactaccg cacgttcaag ccggaaaagg
     1381 gcgggctttt ctgcgaacgc atctttggcc cggtcaagga ctgggagtgt tcctgcggca
     1441 agtacaagcg cattaagcat cggggtgtgg tgtgcgaccg ctgcggcgtc gaggtcacgc
     1501 tctcccgcgt ccgtcgggag cggatggggc acatcgatct ggcggtgccg gtgtcacaca
     1561 tctggttttt caagtgcatg ccttcgcgga tcggactgat gctcgacatg acagcccgca
     1621 atctcgagcg cgtgatctac tacgaggact atctggtcat cgacggcggc gacacgccgc
     1681 tggtccgcca ccagttgctc agtgaaatgg agtaccgcga ggcccgcgag acctacggtc
     1741 cagaggcgtt cacagccaag atgggcgccg aggccgtccg cgacgcactc cagaacatcg
     1801 agttgcagaa gaccatcgac gaactgcaac aggcgatgac cgagacgcgc agtaagcaga
     1861 tccgcaagaa gctcgccaaa cggatcaagc ttttccaggg attcctgact tctcggaccc
     1921 gtccggaatg gatgatcctg accgtcctgc cggtgattcc gcccgacctg cgtcccttgg
     1981 tgccgctcga gggcggacgg tttgcgacct ccgatctcaa cgatctttac cgccgggtca
     2041 tcaatcgcaa caaccgcctt aagaacctcc ttcagctcaa gacgccggag gtcatcatcc
     2101 gcaacgagaa gcggatgctc caggaggcgg tggacgccct cttcgacaat gggcggcatg
     2161 gcagggccgt caccggggca ggcaaccgcg ccctgaaatc gctctccgac atgctgaagg
     2221 gcaaaagcgg gcgattccgt cagaacctcc tgggcaaacg ggtcgattac tccggacgct
     2281 cggtcattgt gatcgggccg gaactcaagc tgcaccaatg cggtttgccc aagaagatgg
     2341 ccttggtgct tttcgagccg ttcattatcc ggcgcctgaa ggaattgggg tacgtgcaca
     2401 ccgttcgctc ggccaagaag atgatcgagc gccaatcgcc ggaagtctgg gacattctcg
     2461 aagaggtgac caaaggacac ccggtgctgc tcaaccgggc gcccaccctg catcgattgt
     2521 ccatccaggc gttcgagccc gtgctgatcg agggcgaggc gatccgggtc catcccctgg
     2581 tctgcaccgc ctacaacgcc gatttcgacg gcgatcagat ggccgtgcat gtgccgttgt
     2641 ccgtcgaggc ccagttggag acccggctgc tgatgatggc cccgctcaat gtgttcagcc
     2701 cttcgagcgg gcgcccgatc atgacgccga cccaggacat caccttgggg tgttattacc
     2761 tgaccgccga accgcggacg ccccgcaagc cccggcagcg cctcatgctt tttgggtcga
     2821 aggccgaggc gctcaccgct catgtggacg gcgccctggg catgcacgat cgcgtgctgc
     2881 ttgcgaatcc ggaccgcggc cggcagacca cctacggcga accggaccgg tccaccatcg
     2941 agacgacggt tggccggatc attttcagcg agatctggcc gcccgaactc ggcttcttca
     3001 acaaggtggc tggcaaggcg caactgggcc agatcatctg gaactgctac aaactgtgcg
     3061 gtcatgagcg caccattgtg gccctcgacc gcctcaagga ggtcggcttc cgcgaggcga
     3121 ctggctcggg atgttccatc ggcatcgacg acatgatcat tcccaaggag aaggatcaag
     3181 agattgccgc tgcccagagg cagattggcg aggtcgagaa gcagtaccgc cgcggcatca
     3241 tcacgccggg cgagcggtac aacaagatca tcgacatctg gacgcactgc acggatcaga
     3301 tctcgaatgt gatgatccag accctcgagt ccaatcaggg caaacgagaa tacaacccgg
     3361 tttggctgat ggtcgattcg ggggcgcgcg gcaacaaggc ccaggtccgg cagttggccg
     3421 gggtgcgtgg gctgatggcc aagccggacg gatcgatcat cgagaagccg attgtgtcga
     3481 atttccgcga gggcctcaca gtgctcgagt acttcatctc cacgcacggg gcccgcaagg
     3541 gcctggccga caccgcgctg aaaaccgccg attccggtta catgacgcgc aagctggtgg
     3601 atgtggccca ggacgtcatc atccgcgagg aggactgcgg gacgaccaac ggcatctggg
     3661 tgcacgcgat ctacgaaggg gaggacgaag tcgtcaagct gaccgatcgg atcgtcgggc
     3721 gggtggcatg cgacgacatt ccggatccgc gcgacccgaa gcacaaactc gtccaggcca
     3781 atcaggagat cgacgaacag atcgccaggg agatcgaggc gtcggatctg gaacgggtgc
     3841 gcatccggtc cgtgctcacc tgcgaaagca agaagggcgt ctgcgctgcg tgctatggtc
     3901 ggaacctcgc caccggcggc aagatcaaga tcggcgaagc ggtcggcatc atagccgccc
     3961 agtcgatcgg agagccgggc acccagctca ccatgcggac gttccacatc gggggcacgg
     4021 cttcgcaggt gttcaaacag ccgcagatca aggccaagta cgacggcatc gtgcgataca
     4081 atgatct
//
LOCUS       DAOQRO010000314         4069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_23837_length_4069_cov_3.894121, whole genome shotgun sequence.
ACCESSION   DAOQRO010000314 DAOQRO010000000
VERSION     DAOQRO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23837_length_4069_cov_3.894121"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..518
                     /locus_tag="PKK20_10990"
     CDS             183..518
                     /locus_tag="PKK20_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNV00455.1"
                     /translation="MKPQETGVTMENPLPSELSYWARMVQRKVTKYWAVPGGIRIDPN
                     NNQAKIAFWIDRQGNLIGKPEVVQHANDPALAESGLRAVQLATPFPQLPDTYMEPEVQ
                     VIYTFTLVR"
     gene            562..1827
                     /gene="tolB"
                     /locus_tag="PKK20_10995"
     CDS             562..1827
                     /gene="tolB"
                     /locus_tag="PKK20_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNV00456.1"
                     /translation="MCAPLAFGQVEIGTTGQAEGRIPIAVPDCCTAPGQEAIGKAMAR
                     VLAYDLYFSGVFGVVPPNAYPRTFTGFTRDPQTIDFEAWRSTPAEFLVHAYVTSDAQS
                     LTAECRLFDVLSGEMVSGKRLATRGQEWHRLVAHQFADEIVRHLTGVAGCASSRICFS
                     GGSTGKKEIYIADYDGANMKQVTQHGSISILPKFSPDGRKIAYLSYKDRYPFLYVLDL
                     DTGESRPLSKQVGLNVAPAWSPDSRQLAIVQSKDGNSEIYLVNVDGSNPRRITNDSAL
                     DTSPAFDPSGTRIAFVSERGGAAQIFVMAADGSGVKRISYQGGRAYDPVWSPDGSKIA
                     YVVDKSGDGFEIYVMNADGAGAKRMTNSAGNNESPTWSPDSRHVMFASSRSGRWELGA
                     VNVESGEEQRIAIDAELSLQGPSWGPRRQ"
     gene            1933..2394
                     /gene="smpB"
                     /locus_tag="PKK20_11000"
     CDS             1933..2394
                     /gene="smpB"
                     /locus_tag="PKK20_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNV00457.1"
                     /translation="MGEKTIVQNRRARHDYHILEQFETGIALQGTEVKSIRAGQITLK
                     DSYAEVDGGELFLIGVHIAPYEQGNIYNHDPERRRKLLMHKREIVRIGHTIAEKGFTL
                     VPLRVYFKDGRAKVELGLCRGKHTIDKRQTLRKREQDRDVDRALKDLKRKK"
     gene            complement(2430..3953)
                     /locus_tag="PKK20_11005"
     CDS             complement(2430..3953)
                     /locus_tag="PKK20_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00458.1"
                     /translation="MNVDVVLAVVNESEALLEARINVISEAYGLDISRTKSGHYAVRG
                     AHRWHARSERDRTFLIEIKVSGYKPRVLEFGRDFQAKEDHLCVVLQEGPFERRESVFS
                     SVTGRPPTDEEVAKMPHARRVRLRESEYRRVAAEGHVCPEELPPKPKEHGISLQGRHY
                     PPPPECGSLIPVMARSLEGVAGLPCVAAVDGRGSPVDRIFVQWAMHMGPDRVTGVPRG
                     VLFKDAPVRTLSSPDGKFRVPNNCVIWSEGTEQVFVPAPYSDDPGLREVILPLSRESK
                     GKWVARRRLGQGEDRPKASRAEIREIIEDLEDSPGLNGSAREERFHKALEQARMYRVT
                     QAARTIRTLLLKWEFKGDFDFWYTDELVATLVDIQGDGAIRFLEQTASDTTVNPLARR
                     SAVIGLGRIGSERSVAAFERLRDAARSLPEAPQAQESYTHQERICEAVEMVCIWIPGV
                     DPKSRVAFQPIARTASVSGDYITASVHCGSPFELTEVELKRFGDEWLVIEIGGTLCF"
BASE COUNT          874 a         1403 c         1089 g          703 t
ORIGIN      
        1 agccgaaacc ggagccgaaa ccggagccga aaccggagcc gaaaccggag ccgaaaccgg
       61 agccgaagcc cgagccgaag cccgagccaa aacctgagcc caagcctgag cccaagcccg
      121 agcggaccct ggagccgccg gacgagcctc tcgaaccgcc ggcagaacca attgccgcgc
      181 ccgtgaaacc gcaggaaacg ggcgtgacca tggagaatcc gctcccctcc gagttgtcct
      241 actgggcgcg gatggtccag cggaaagtaa cgaaatactg ggcggtgccg ggcgggattc
      301 gcatcgaccc gaacaacaat caggcgaaga ttgccttctg gatcgaccgg cagggcaatc
      361 tgatcggcaa accggaggtg gtgcagcatg ccaacgatcc cgcgctggcg gagtccggat
      421 tgcgggcggt gcagttggcc acgcccttcc cccagttgcc ggatacctac atggaacccg
      481 aagtacaggt catttacacg ttcaccctgg tacgatagga gacggatcgt gaaaacttgg
      541 tgtctcactg caattgttgc gatgtgcgcc cccctcgcct tcgggcaggt cgaaattggc
      601 accaccggac aggccgaagg ccgcatccca atcgccgtgc cggattgctg caccgccccc
      661 ggccaggagg ccatcggcaa ggccatggcg cgcgtcctgg cctacgacct ttatttctcg
      721 ggcgtcttcg gggtcgtccc ccccaacgcc tacccccgca cgttcaccgg gttcacccgc
      781 gacccgcaaa ccattgattt cgaggcctgg cgaagcaccc ccgccgagtt cctggtccac
      841 gcctacgtga cctccgacgc acaatccctc acggccgagt gccgcctctt tgacgtgctg
      901 tcgggcgaga tggtcagcgg aaaacgcctc gccacccgcg gccaggaatg gcaccgcctc
      961 gtcgcccacc agttcgctga cgaaatcgtg cggcacctca ccggcgtcgc cggctgcgcg
     1021 tcctcgcgca tctgtttcag cggcggcagc acgggcaaaa aagagatcta cattgccgac
     1081 tacgacggcg ccaacatgaa gcaggtcacc cagcacggct ccatctcgat ccttcccaaa
     1141 ttctccccgg acggccgcaa gatcgcctac ctctcctaca aagaccgcta cccgttcctc
     1201 tacgtgctcg acctggacac cggcgaatcc cggcccttgt ccaaacaggt cggcctgaac
     1261 gtcgccccgg cctggtcgcc cgacagccgc cagctcgcga tcgtccagag caaagacggc
     1321 aacagcgaaa tctacctcgt caacgtcgac gggtcgaacc cccggcgcat caccaacgac
     1381 agcgccctcg atacctcccc cgcgtttgat ccgtccggca cgcgcatcgc cttcgtcagc
     1441 gagcgtggcg gcgccgcgca gatcttcgtc atggccgccg acggctccgg cgtaaaacgc
     1501 atctcctacc agggcggccg agcctacgac cccgtctggt cgcccgacgg ctccaaaatc
     1561 gcctacgtgg tcgacaaatc cggcgacgga ttcgaaatct acgtcatgaa cgccgacggc
     1621 gccggcgcaa aacgcatgac caactccgcc ggaaacaacg aatcgcccac ctggtcgccc
     1681 gactcgcgcc acgtcatgtt cgcgtccagc cgcagcggac gctgggaact cggcgccgtc
     1741 aacgtcgaat ccggcgaaga acagcgcatc gccatcgacg ccgaactctc gctccaggga
     1801 ccctcctggg gaccgcgcag gcaataacca cgcgcaaaaa acggaccggc tctgaaaacg
     1861 ccgcatcgaa cggccgcacc gcgccccggg cgcgttgaaa acccggcccg tttgcgccac
     1921 actggaacca ccatgggcga aaaaaccatc gtacagaacc gccgcgcgcg gcacgattac
     1981 cacatcctcg aacagttcga gaccggcatc gccctccagg gcaccgaggt caaatcgatc
     2041 cgcgccggac agatcaccct caaagacagc tacgccgagg tcgatggcgg cgaactcttc
     2101 ctcatcggcg tccacatcgc cccctacgag cagggcaaca tctacaacca cgaccccgaa
     2161 cgccgacgca aactcctcat gcataaacgc gagatcgtgc gcatcggcca caccatcgcc
     2221 gaaaaaggct tcaccctcgt cccgctccgc gtctatttca aagacggccg cgccaaagtc
     2281 gaactcggac tctgccgcgg caaacacacc atcgacaagc ggcaaaccct ccgaaaacgc
     2341 gaacaagacc gcgatgtcga ccgcgccctc aaggatctca agcggaagaa atagaacttc
     2401 cagctatcgt ggctggaaaa gaatgcgagt cagaagcaca acgttccgcc gatttcgatt
     2461 acgagccact cgtcgccgaa tcgcttcaac tccacctccg ttaactcaaa aggcgacccg
     2521 cagtggacag acgccgtgat gtaatctccg ctcacggacg cggtgcgtgc tatcggctga
     2581 aaagcgactc gcgacttggg gtcaactcct ggaatccaga tgcataccat ctccacagct
     2641 tcgcagatgc gttcttgatg cgtataggat tcttgagctt gcggcgcttc aggaagcgac
     2701 cgggcagcat cccgcaggcg ctcaaacgcg gcgacactgc gctccgaccc gatccgccca
     2761 aggccgatga cagcgcttcg ccgtgccagc ggattcacgg tggtatccga tgctgtctgc
     2821 tccaggaatc ggattgcccc atcgccctgt atatcgacca gtgtcgccac cagttcatcc
     2881 gtataccaga aatcgaagtc tcctttgaac tcccatttca gaagaagagt tcgaatcgtt
     2941 cgcgcggctt gggtgacacg atacatgcgg gcctgctcga gcgccttatg aaagcgttct
     3001 tctctggcac ttccattcag accgggggaa tcctcgagat cctcaatgat ctcccgtatc
     3061 tcagcgcgcg aggccttcgg acggtcctcc ccttggccga gtctccgacg tgctacccat
     3121 tttcccttcg actccctgga gagcggcagt attacctccc gcagaccggg atcatccgaa
     3181 tagggcgccg gtacaaacac ttgttccgta ccctcagacc atataacgca gttatttggc
     3241 accctgaatt tgccgtccgg gctgcttagc gttcgtacag gcgcatcctt gaacaggaca
     3301 ccgcgtggca caccagtcac tctgtcagga cccatgtgca ttgcccattg gacaaagatt
     3361 cggtcgacgg gagaacccct tccgtcaacg gctgcgacgc acgggagtcc tgcaacgcct
     3421 tctagcgaac gggccatgac tggtatcaaa gatccgcatt cgggaggtgg cggatagtgc
     3481 cgaccctgca agcttatgcc gtgttctttc ggcttcggag gcagttcctc gggacacaca
     3541 tgtccctcgg cggccacgcg gcgatactca gattcacgga gtcttactct gcgcgcgtga
     3601 ggcatcttgg ccacctcctc gtctgtcgga ggacggcctg tgacggatga gaagacagac
     3661 tcgcgccgct cgaaagggcc ttcttgcagt acaacacaca agtgatcttc ctttgcctga
     3721 aaatctctgc cgaactccag aacccgcggc ttgtagccac tcactttgat ctcgatgaga
     3781 aatgtgcggt cccgttcact tcgcgcgtgc cagcggtgag ctcctcgcac tgcgtagtgc
     3841 ccgctcttag tacgactaat gtccaacccg taggcttccg agatgacatt gatccgtgct
     3901 tccagcaaag cctcggattc gtttaccaca gcaaggacga cgtccacatt cacagaatct
     3961 ccgttatcgc tttgtgcgtt ttgggccacg aggccaagca ggagcgaagc ggccgtcagc
     4021 cgagccagcc atttcttttg aaccaacacg ttctatcgaa cctccgctt
//
LOCUS       DAOQRO010000315         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_23862_length_4066_cov_5.331090, whole genome shotgun sequence.
ACCESSION   DAOQRO010000315 DAOQRO010000000
VERSION     DAOQRO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23862_length_4066_cov_5.331090"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKK20_11010"
     CDS             <1..404
                     /locus_tag="PKK20_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00459.1"
                     /translation="VKYVDTVCRATKERQWAAVELAQGCDVVVVVGGARSNNTRQLAA
                     TCARHCARVHHVESPEEVRPEWFRAADTVGLTAGTSTPDDVIAGVDARLRAIARVADA
                     NPGPDAQAELLAAAVPLGEIERAPASCLATP"
     gene            410..1501
                     /gene="hemH"
                     /locus_tag="PKK20_11015"
     CDS             410..1501
                     /gene="hemH"
                     /locus_tag="PKK20_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNV00460.1"
                     /translation="MKGVLLVNLGSPDSPAVGDVRRYLREFLMDGRVVDIPWLARFFL
                     VHGIILPRRPQQSAEAYRKIWTPEGSPLVASSRRLAAALRERVGVSVGLAMRYRNPSI
                     SDALEGLEAEGVTEVLLIPLFPHYAMSSYETAVERVLELARRRGGPRIEVVPPYYDHP
                     QYIRALVATAAEHLTGDYDRLLFSFHGIPERHLRKSDPTAHHCLGRADCCAVASPAHR
                     TCYRRQCFRTAEAFVEAAGIPGSRWSVAFQSRLGRAAWLKPYTDEELARLAAGGVKRL
                     LVICPAFVTDCLETIEEIGLRGRELFVGAGGQDLIRIPCLNEHPAWLDALTRMVERFT
                     LGPCARAGSVYKEKKHATIRHESGSMDGD"
     gene            1473..2945
                     /locus_tag="PKK20_11020"
     CDS             1473..2945
                     /locus_tag="PKK20_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV00461.1"
                     /translation="MNQGPWTEIDAQHDWYNLPRSAPPKRSVKERVADFLEVYGDYDE
                     ATAREQARRCVECAEPLCVSGCPLGNRIPEWMLLTAEGQFLEAAALSRSTSNMPEICA
                     RVCPQERLCEGACVLNGRAEPVAIGAIERFINEYAFAHGAVDPVPAPPNGMRVAVLGS
                     GPGGLSCADELARRGYAVTVYESQPTLGGLLVNGIPAFKLEKSVVARRVSLLEKRGVV
                     FKVGVTVGRDISLTGLLLEHDAIFLGFGAQQARPLGIPGADLAGIYDALPFLVQKNTD
                     TPLDMPPIDVRGKRVAVLGGGDTAMDCLRTAIRCGAAEALCIYRRDRANMPGSRKEYD
                     NSIEEGARFSFLTSPVSLLGDAEGRVTTVRCVRMELGAPDSSGRRSPRPVRGSEFDVP
                     VDVVLTAYGFELVPFPPGSDLATIKRNDRGEIVVDDQQMTSMSGVFAGGDLVRGPSLV
                     VHAVRDARRAALGIHRYLSHRRMFELDADQTDHLSPELTT"
     gene            3027..3656
                     /locus_tag="PKK20_11025"
     CDS             3027..3656
                     /locus_tag="PKK20_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNV00462.1"
                     /translation="MGLAAVLEASAADSHSRWENDIQAFEASDRTNAPPSNAILFVGS
                     SSIRLWKTLAEDFPEWPVINRGFGGSQVADSVAFVDRIVTPYRPRQIVLYAGDNDLAA
                     ERTPEQIEADFKTFVRRVHKRLPRCWITFIAIKPSPARQTLIEKARAANERVREVTRA
                     HPRLSFVDIFTPMLGADGQPRPELFMADKLHLNVEGYRLWTRLTRPYLK"
     gene            complement(3864..>4066)
                     /locus_tag="PKK20_11030"
     CDS             complement(3864..>4066)
                     /locus_tag="PKK20_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00463.1"
                     /translation="DPISNRPFWASFISPVAHYPYHQILHIIEAPDGISTGIVFTNHF
                     SVVIETMQLVVYSGAVGASIPI"
BASE COUNT          699 a         1193 c         1420 g          754 t
ORIGIN      
        1 aggtgaagta tgtggacacc gtgtgccgcg caaccaagga gcggcagtgg gcggcggttg
       61 aactggcgca ggggtgcgac gtcgtggtgg tcgtcggcgg cgcccgcagc aacaacacac
      121 gccagctcgc ggcgacctgc gcgcggcact gcgcccgggt ccaccacgtc gaatcgcccg
      181 aggaagtgcg gcccgagtgg tttcgagccg ccgataccgt cggcctcacg gccgggacgt
      241 ccacgcccga cgatgtcatc gccggtgtcg acgcccggtt gcgggccatt gcgcgggtgg
      301 ccgatgccaa tcctggcccg gacgcccagg cggaactgct ggccgcggct gttcccttgg
      361 gcgaaatcga aagggcgccc gcgtcctgtc ttgccacgcc ttagggtctg tgaagggcgt
      421 tttgctggtc aatctgggat cgccggattc tcccgcggtg ggggatgtcc ggcggtattt
      481 gcgagagttt ctgatggacg ggcgcgtcgt ggacattccg tggctggcgc ggttctttct
      541 ggtgcatggg atcatactgc cgaggcgccc gcaacaatcg gccgaggcgt accgaaagat
      601 ctggacgccg gagggctcgc cgctggtcgc gagcagtcga cggttggccg cggcattgcg
      661 ggagcgcgtt ggtgtgtcgg tcggattggc aatgcgctat cgcaacccgt cgatttccga
      721 tgcgctcgaa gggctcgagg cggagggtgt gaccgaggtt ctgctgatcc cgctgtttcc
      781 gcactacgcg atgtcgagtt acgagacggc tgtcgagcgg gtgctggagc tggcgaggcg
      841 gcggggcgga ccgcggatcg aagttgtgcc gccctactac gatcatcccc agtacatccg
      901 ggcgctggtg gcgactgcgg cggagcactt gacgggcgac tatgaccggc tcttgttcag
      961 ttttcatgga atccctgagc gccacctgcg aaagagcgat ccgacggccc atcactgtct
     1021 gggccgcgcg gattgttgcg ctgtcgcgag tcctgcacac cggacgtgct atcgccgcca
     1081 gtgttttcga accgccgagg ccttcgtgga agcggcgggc attcccggat cgaggtggtc
     1141 cgtggccttt caatcgcgcc tggggcgggc tgcgtggctg aagccgtata cggacgagga
     1201 attggcgcgg ctggctgccg ggggtgtgaa gcggttgttg gtgatatgtc ccgcgtttgt
     1261 gaccgattgt ctcgagacga tcgaggagat tgggctgcgt ggacgcgagc tgttcgtggg
     1321 tgccggcggc caggacctca tccgcatccc gtgtctgaac gagcatccgg cgtggctcga
     1381 tgccctgacg cggatggtgg agagatttac cctcggccct tgcgctcgag ccgggagcgt
     1441 gtacaaagag aaaaaacacg ctacaattcg gcatgaatca gggtccatgg acggagattg
     1501 acgcccagca cgactggtac aacctgccgc gatcggctcc gccgaaacgt tcggtgaagg
     1561 agcgggtggc cgactttctc gaagtctacg gagattacga cgaggcgacg gcccgcgagc
     1621 aggcgcgccg gtgcgtggaa tgcgcggaac cgctttgcgt gtccggatgc ccgctgggga
     1681 accggattcc ggagtggatg ctgctgacgg cggaagggca attcctcgag gcggcggcgc
     1741 tgtcgcgttc caccagcaac atgcccgaga tctgtgcgcg ggtctgcccg caggagcgcc
     1801 tgtgcgaggg agcgtgtgtg ctcaatggac gcgccgaacc cgtcgcgatc ggagcgatcg
     1861 aacgcttcat taacgaatac gctttcgctc acggagcggt cgatcccgtc cctgcgccgc
     1921 ccaacggaat gcgcgtcgcc gtgctagggt cgggtccggg cgggctgtcg tgcgccgacg
     1981 aattggcccg gcgcggctat gcggtgacgg tgtacgagtc ccaaccgacg ctgggcggtc
     2041 tgctggtcaa cggcattccg gcattcaagc tcgagaaatc ggtggtggcg cgcagggtca
     2101 gcctgctcga gaaacggggg gtggtcttca aagtcggggt gacagtgggg cgcgacatca
     2161 gcctgacggg attgctgttg gaacacgacg cgatcttctt gggatttgga gctcagcagg
     2221 cgcggccctt gggcatcccg ggggcggatc ttgcaggcat ctacgacgct ctgcccttcc
     2281 tggtgcagaa gaacaccgat actccgctgg acatgcctcc catcgatgtg cgcggcaagc
     2341 gggtggcggt tctgggggga ggcgacaccg caatggactg cctgcgcaca gccatccgct
     2401 gcggggcggc tgaggccctc tgcatttacc ggcgggaccg ggcgaacatg cctggcagcc
     2461 gcaaggagta cgacaactcg atcgaggagg gggctcgttt cagtttcttg accagtccgg
     2521 tctctctcct gggcgacgcc gaaggccggg tcacaaccgt tcggtgcgtg cggatggagc
     2581 tgggggcccc ggactcgagc gggcgccgct cgcctcggcc cgtgcgcggt tcggagtttg
     2641 acgtgccggt ggacgtggtt ctgacggcgt acggattcga gttggtgccg tttccgccgg
     2701 ggagcgatct ggccacgatc aagcgcaacg acagggggga gatcgtggtg gacgaccagc
     2761 aaatgaccag tatgagcggg gttttcgcag gcggcgatct ggtgcgcggg ccgagcctgg
     2821 tggtgcatgc ggtccgggac gcccgccgcg cggcgctcgg cattcaccgt tacctgtcac
     2881 accggcggat gttcgaactc gacgcggacc agaccgatca cctgagtccg gaactcacca
     2941 cctgacgcgc gtctatgaaa acagaatggg ttgctcatgg aaaggcgcgc gcaggttgga
     3001 tgtggggctt gtcggtgtgt ttgccgatgg gcttggcggc cgtgctcgaa gcgagtgcag
     3061 ccgattccca ttcgcgctgg gaaaacgaca ttcaagcgtt cgaggcatcc gaccgcacca
     3121 atgcgccacc gtcgaacgcg attctcttcg tcggaagttc gtcgatccgg ctttggaaga
     3181 ccctggccga ggattttccc gaatggccgg tcatcaatcg aggttttggc ggctcgcagg
     3241 tggcggactc cgtggcgttc gtcgatcgga tcgtgacgcc gtaccgtccg cggcagattg
     3301 tgctctacgc gggcgacaat gatcttgcgg cggaaaggac gcccgagcag atcgaagccg
     3361 atttcaagac gttcgtccgc cgggtgcaca aacgcctgcc ccggtgctgg atcacgttca
     3421 ttgcgatcaa accaagtccg gcgcgccaga ctctcatcga gaaggcccgc gccgccaacg
     3481 aacgcgtccg cgaggtcaca cgagcccacc ccaggttgtc atttgtcgat atcttcaccc
     3541 cgatgctcgg ggcggacggc cagccgcgtc cggagttgtt catggccgac aagcttcacc
     3601 tcaatgtcga gggctaccgg ctttggacgc ggttaacgcg tccgtatctc aagtagggtc
     3661 tgcgcggcga ggcgtgcggc ttgtcggagc ccggtttcct atctcaggaa gccccagccc
     3721 cgcgagtcag cgcgaaccac gaagcttcac gcccgaccaa cgtccggctc accgatgacg
     3781 aagagcgcgc caaggacacc cagattggag ccgggacgtg cccgcgctcc tcagcattcg
     3841 gtcaagcgtc ttcggcagga taatcatata ggaatggaag ctccaactgc cccagaatac
     3901 acgacgagct gcatcgtctc gatcacaacg gagaagtggt ttgtaaaaac aatacccgtg
     3961 gatatgccat ccggtgcctc gatgatatgg agaatctggt ggtatggata atgtgccacc
     4021 ggcgagatga aactggccca aaacgggcgg tttgaaatgg gatctt
//
LOCUS       DAOQRO010000316         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_23963_length_4055_cov_4.749000, whole genome shotgun sequence.
ACCESSION   DAOQRO010000316 DAOQRO010000000
VERSION     DAOQRO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23963_length_4055_cov_4.749000"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKK20_11035"
     CDS             <1..199
                     /locus_tag="PKK20_11035"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008487959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNV00464.1"
                     /translation="ARRAHQLGLRVNAGHGLNYRNLPALLEVPHLVELNIGHAIISRS
                     VVVGLARAVRDMLALMRPYDG"
     gene            189..617
                     /gene="acpS"
                     /locus_tag="PKK20_11040"
     CDS             189..617
                     /gene="acpS"
                     /locus_tag="PKK20_11040"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019110380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNV00465.1"
                     /translation="MMDEHPQPPAPDAPPQTVLGIGVDIIEVERIRSSAEKFGDRFLT
                     RILRPTELAYCATFRHPAPHWAARFAAKEAISKAFGTGIGAKLGWLDMEVLRHPSGQP
                     YAALHGRGAVLLRERGGSALHLTLSHTAHHAVAVALLERG"
     gene            703..1083
                     /locus_tag="PKK20_11045"
     CDS             703..1083
                     /locus_tag="PKK20_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HNV00466.1"
                     /translation="MSQTDRLSEIFRLQRSLNERIGVQTSGMSQADKVTWTLNYCRAM
                     SQEIAELTDSVPWKWWAKYQEFDEQNARVEVVDLFHFLISLAQVLGMDAEDVYAAYLK
                     KNQVNVQRQEHGYAHKDINDSRHI"
     gene            1158..1778
                     /gene="hpt"
                     /locus_tag="PKK20_11050"
     CDS             1158..1778
                     /gene="hpt"
                     /locus_tag="PKK20_11050"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNV00467.1"
                     /translation="MWARPRRVTQTHVVPPQWRADLREVLITEAQIKRRVAELARAIT
                     RDYVGRDPLVVALLTGTLMFLADLLRRLAVPLKLDFIGVSSYREGTISRELVFTKDLR
                     LEVAGRDVLIVDDILDTGRTLKTVRARLEKLKPGSLRTCVLLDKPDRRTEPIEADYVG
                     FVIPDLFVVGYGLDYAERYRNLPFVGVLRSELYQVPPKETGLGPTT"
     gene            complement(1820..3202)
                     /gene="larC"
                     /locus_tag="PKK20_11055"
     CDS             complement(1820..3202)
                     /gene="larC"
                     /locus_tag="PKK20_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNV00468.1"
                     /translation="MKAVYFDPFSGVSGDMILGALLDLGVEIGSLRKDLARLPLGGYH
                     LHATRRTKAGICGTKFDVHLGEAQEHSHAAGDEGHHHEPQTAPDAQSGMHKGHTHPHP
                     GHSQPSIPDRCHVHSGPHHGEAEVRDFRSIATMVSGSSLPGPVRDRALGVFRRIAEAE
                     GKIHGCPPESVHFHEVGAVDSIVDVVGGCLALEALGRPRVFAAPLIEGTGWVRCAHGQ
                     FPVPAPATLEILAARGLTLRQCEEPQEMVTPTGAAFLAEFAEAFGPMPAMRLERIGYG
                     LGMHDMRTRPNVLRAVLGELESEPGAPVLPHDWEGDHVGCLETNLDDANPEVLGHFVD
                     RALAAGALDVFYGSVQMKKNRPGVVLTVLCPVAECDRFSELILCETTAFGVRRTVLER
                     RKLRREFLSVDTPVGRIKVKVGRLDGRIVQVAPEYESCRAVAAERGMPLQAVYTAAVR
                     AVPASSGDSR"
     gene            complement(3199..3960)
                     /gene="larB"
                     /locus_tag="PKK20_11060"
     CDS             complement(3199..3960)
                     /gene="larB"
                     /locus_tag="PKK20_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNV00469.1"
                     /translation="MTTNDAVRLLEQFRAGAVTRAQVLRAFRAPAVSNLGFARVDLDR
                     GLRKGFPEVIFGEGKTPAQVVAIARQIWRHEGRVLVTRTTPGHARAMKRAFPRAVHHE
                     NARCITLERTPLPKRGGPITVLCAGTTDLPVAEEAAVTAQIMGNRVERVYDVGVAGLH
                     RLLGSLEAIQNAHVIIAVAGMEGALPSVVAGLVSRPVIAVPTSVGYGASLGGLAALLA
                     MLNSCASGVTVVNIDNGFGAGYAASQINALSRDTA"
BASE COUNT          759 a         1341 c         1259 g          696 t
ORIGIN      
        1 cgcccgacgc gcccatcagc tcggcctgcg ggtgaatgcc ggccacggcc tcaactaccg
       61 caacctgccg gcgctcctcg aagttccgca ccttgtggaa ctgaacatcg gccacgccat
      121 catcagccgg tcggtcgtgg tcggtctggc acgggctgtc cgcgacatgc tggccctcat
      181 gcggccctat gatggatgag catccgcagc cgcctgcccc ggacgcaccg ccccagacgg
      241 ttctaggcat cggcgtcgac attatcgaag tcgagcgcat tcgatcgagc gccgagaagt
      301 tcggcgatcg ctttctgact cggatactgc ggccgacgga attggcctat tgcgccacct
      361 tccgtcaccc ggcgccgcac tgggcggccc gattcgccgc gaaagaggcg atttccaagg
      421 cgtttgggac ggggattgga gccaagttgg gatggctgga catggaagtt ctgcggcacc
      481 cgtcggggca gccttacgcc gcgcttcatg gccgaggggc ggtcttgctt cgggagcgtg
      541 ggggaagcgc tctccatctc acgctcagcc atacagccca tcacgccgtg gccgtcgccc
      601 tgctcgaacg gggctaacca ctacccctcc gccaatccgc caatccgcca tacccgccag
      661 ttccggcttg cgtcctttcg acacactccg aatgctcctg ccatgtcaca gactgaccga
      721 ttgagtgaga ttttcaggct gcagcggtcc ctgaacgagc ggattggggt gcaaacgtcg
      781 gggatgagcc aggccgacaa agtgacgtgg accttgaact actgccgtgc gatgagccag
      841 gagatcgccg agttgaccga cagcgtcccc tggaaatggt gggccaagta tcaggagttc
      901 gacgaacaaa acgcgcgggt cgaggtggtc gatctgtttc acttcctgat cagccttgcc
      961 caggtgctcg gcatggacgc cgaagacgtc tatgcggcct atctcaagaa gaaccaggtg
     1021 aatgtccagc gccaggaaca cggttacgca cacaaggata tcaacgattc acgccacatt
     1081 tgaaccctcg cactgtccca tcgccttcgc caagaccgcc cgacgacgcc ccgcgacccg
     1141 ccgcctccgc atccgaggtg tgggcacgcc ctcggcgcgt cacccaaacc cacgtggtgc
     1201 ctccccagtg gcgggccgac ctgcgcgaag tgttgatcac cgaagcccag atcaaacggc
     1261 gggtcgcgga gctggcgcgc gcgatcactc gcgactacgt gggccgggat ccgcttgtcg
     1321 tggcgctcct gaccggcacg ttgatgttcc tggccgacct gctccggcgt ttggcggtcc
     1381 cgctcaaact cgacttcatc ggcgtctcga gctaccggga aggcacgatc tcccgagaac
     1441 tggtcttcac caaagacctg cgcttggaag tcgcgggacg cgatgtgctg atcgtggacg
     1501 acatcctcga caccggacgc accttgaaaa cggtccgtgc aagactcgaa aagctcaaac
     1561 cgggatcgct ccggacttgt gtgctcctgg acaagccaga ccggcgtacc gagcccatcg
     1621 aagccgatta tgtcggcttt gttatccccg accttttcgt tgtggggtat ggactcgact
     1681 atgccgaaag gtatcggaat ctcccgtttg tgggggtgtt gcgctccgag ctgtaccagg
     1741 tcccgcccaa agagacaggc ctcggcccga caacctgaca gggctccgca cggtcgaatc
     1801 ttggggccgc agggctggac tacctcgaat cgcctgacga ggccgggacg gcccgaaccg
     1861 cggctgtgta aacggcctgg agaggcatcc cccgctctgc cgcgaccgcg cgacaggatt
     1921 catactcagg agccacctgc acaatccggc cgtccaggcg tcccaccttc accttgatcc
     1981 gtccgacagg cgtgtcgaca ctcaggaact cccgccggag tttccgacgc tcgagaacag
     2041 tgcgccgcac accaaaggcg gttgtttcgc acagaatcag ttcgctgaac cgatcgcact
     2101 cggccaccgg acacagaacc gtcaaaacca cacccggacg gttcttcttc atctgaacgg
     2161 agccgtagaa aacatcgaga gctccggccg ccagtgcacg gtcgacaaaa tgccccagca
     2221 cttccggatt ggcgtcgtcg agattcgttt ccaaacagcc aacatgatcc ccctcccagt
     2281 catggggcag caccggggcg ccaggttcgg attcgagttc gcccagaacg gcgcgcagca
     2341 cattgggacg cgtcctcatg tcgtgcatgc ccaggccata gccgatccgc tccagtcgca
     2401 tggcgggcat gggaccgaag gcttccgcga actccgccag gaaggccgcg ccggtcggag
     2461 tgaccatctc ctgcggctct tcacattgtc tgagggtcag gccccgcgcg gcaagaatct
     2521 cgagagtggc cggcgccggc acaggaaatt gcccgtgcgc gcaccggacc catcccgtcc
     2581 cctcgatcaa gggcgccgcg aacacgcgcg gtcgaccaag ggcctcgagc gccaagcacc
     2641 ccccgacaac gtccacgatc gagtccacgg cgccgacttc atggaaatgc accgactcag
     2701 gcgggcaccc atgaatcttg ccttccgcct cggcaatgcg tcggaacacg cccagcgcgc
     2761 ggtcgcgcac cggaccggga agcgaactcc cggaaaccat ggttgcaatg gaccggaagt
     2821 cgcggacttc ggcttcccca tgatgcggtc cggagtggac atggcagcga tcgggaatcg
     2881 agggttggga atggccgggg tgcgggtggg tgtgcccctt gtgcatgccg gactgcgcat
     2941 ccggagcggt ttgcggctcg tggtgatgcc cctcgtcccc ggcggcatgg gaatgttcct
     3001 gcgcctcccc caggtgcacg tcgaacttcg tcccgcagat gcccgccttg gtccggcgag
     3061 tggcatgcag atggtagcca ccaagaggga gacgagcgag atccttccgg aggctgccta
     3121 tctcgacccc caaatcgagc agagctccga gaatcatgtc tccactgacg ccgctgaagg
     3181 gatcgaagta gacggctttc atgcggtgtc tcgactgaga gcgttgattt gacttgcggc
     3241 gtagccggcc ccgaatccgt tgtcgatgtt gactacggtc acgccgctgg cgcaactgtt
     3301 gagcatggca agaagggcgg ccaggccgcc aagactggcc ccataaccga cgctggtggg
     3361 cacggcgatg acggggcgtg agaccagtcc ggcgacgacg ctgggcaaag ccccttccat
     3421 ccccgccacg gcgatgatga catgggcgtt ctggatcgct tcgaggcttc cgaggaggcg
     3481 atgcaagccg gccacgccga catcgtagac ccgttcgaca cggtttccca tgatctgggc
     3541 cgtcacggcc gcctcttcag ccacgggcag gtccgttgtc ccggcgcaca agacggtaat
     3601 cgggccccct cgtttgggca gaggcgtccg ttcgagggtg atgcagcgcg cattctcatg
     3661 atgcacggcg cgtggaaaag cgcgcttcat cgcccgagcg tggccgggcg ttgtccgggt
     3721 cacgagcact cggccctcgt gacgccagat ctggcgtgcg atggcgacga cctgcgccgg
     3781 tgtcttgccc tcaccgaaaa tcacctcggg gaatcctttg cgcaggccgc gatcgagatc
     3841 cacgcgggca aagccgagat tgctcaccgc tggggcccgg aaggcgcgca acacctgggc
     3901 tcgagtgacc gcgccggcgc gaaactgctc caggagtctg acagcgtcgt tcgtcgtcat
     3961 ggggtcaggc tgtaaagtcc gggcgccagc gatccgaatg gatcgatcct cacagcgtag
     4021 cagggcgccc cagcgatgca gttcgcggag gcaac
//
LOCUS       DAOQRO010000317         4048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_24028_length_4048_cov_4.099925, whole genome shotgun sequence.
ACCESSION   DAOQRO010000317 DAOQRO010000000
VERSION     DAOQRO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24028_length_4048_cov_4.099925"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /locus_tag="PKK20_11065"
     CDS             <1..2547
                     /locus_tag="PKK20_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNV00470.1"
                     /translation="VPGVQLTASHNPASYNGIVAMGSLPNAVVLRGTVLVYNNGWHLL
                     AEGGTIQAGRWSHLAFVYAPGHCTLYLDGEPLLSAASGLDFDAAELGLGARLDLEGSL
                     GGPFGTGYGGMMDDVSIWDGALQPSSIRKLASGVSPKLVVDSPATEPPSIFAHPAGAT
                     VLLGTPATLSVVASGARALTYQWEKDGQPVDGATGSTLTFPAVTLADAGEYRVVVNNQ
                     YGTAISHVARLIVDPGVPVLVTQPQSQTKRVGAEAMFTVEATGALPLTYQWHKDGQAI
                     TGAIEPTYHVAAVSAADAGAYTVVVRNVSGSVISEVATLVLVFTPPAVNVDLAVASGQ
                     NVYTGVAAAPDPGTVWNQVTENALRSALDRTLALVDSDGLPSSIGFTSASFGEPGSGL
                     FANNGGGNALQATYWHSGVGNESPPFGFTNLDPAVRYDVYVYGIATDFGLGYTQRISR
                     VGGESQALNQVVNTGFPILHEDYALFRDITGVTEVMFTAGESGNGAVFSTVTGLQIIP
                     ATDTPKILQSPTDRFVRQGASVTFTVVATGTAPFTYQWRKNGVAIQGATGDTYVIASA
                     RADDEGLYSVVVSNAAGSVTSESGRLTLQTAPPAVNINLIAAADSSEYAGTAAAPDVG
                     TVWNQVTEEALRADADREMALVDSDGLPVDARFFSGSFGEPSSTWFAENGGGNFLQRS
                     YWHVGGGKASPEFGFRNLEAGKTYDVYVYGIATDFNLGWGQAYTLVGGPTQTLKQVPD
                     TGFPVVNEDYVVFKTISGVAEIKLTSGQAGAFFSTVAGLQLIESAKGVPATLTITRSV
                     TGEIVIGWTGSGVLEQADAVTGAWKRVANPANPYTAAPAGAARYYRVVQD"
     gene            2782..>4048
                     /locus_tag="PKK20_11070"
     CDS             2782..>4048
                     /locus_tag="PKK20_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00471.1"
                     /translation="MSLGAIRVLGVIVGLAMFTDAARCATVQPRYYGHAAVCDAHGVI
                     APWYRGLNGQCDFRIRVAAETLKRYPWTDTNSAIAAYPDYLFTSLWQIDPDGRITPKA
                     SDAWMNGDLGQRSTSLLNGWVDYYRYSGDPAAVAHLTYLGEFLLDHCLTPPDHPWPGL
                     FISVPAKGKAYGNCDPEGMIQLDICASTGQGLLRAYQLTGRTRWLAAARHWGDLLAER
                     CRKDPGEAPWGRYANPEAAPWKDNQQTGGVTMILAFLDDLIRLGYTGQDNAIVSARDA
                     GRAYLRDRLLPAWTVDDTWGRYFWDWANPTQNCATTPDAARYLLHHQAEFPNWQTDVR
                     NILTLFLNRSGVAPESRGDVYSGAWAFPESSSCCGRSLWYSPFLLATALAEYACHTRS
                     DWARELAYRMMVLQTYDAHETGVTEDNIDG"
BASE COUNT          708 a         1313 c         1322 g          705 t
ORIGIN      
        1 gtgcccggcg tccagttgac cgcctcgcac aacccggcca gctacaacgg catcgtggca
       61 atgggcagcc tcccgaatgc cgtcgttctg cgcggcacgg ttctggtcta caacaacggc
      121 tggcatctcc tcgccgaggg cggcacgatc caagccggcc gctggtcgca cctggcgttc
      181 gtttacgccc ccggccactg cacgctctat ctggacggcg agccgctgct cagcgccgcc
      241 tcgggtctgg atttcgacgc ggccgagctg ggtctggggg cgaggctgga ccttgaaggc
      301 agcctgggcg gcccgttcgg gacggggtat ggcggaatga tggacgacgt ctcgatctgg
      361 gacggcgctc tgcaaccgtc ctcgattcgg aagctggcca gcggcgtctc gccgaagctg
      421 gtggtggact cgccggcgac agaaccgccg agcattttcg cgcaccccgc aggtgcaacc
      481 gtcttgctgg gcactccagc gaccctgagc gtggtggcgt ccggggcccg cgccctgacc
      541 taccaatggg aaaaggacgg ccagccggtg gatggcgcga ccggaagcac gttgacattt
      601 ccggcggtga cgttggctga cgccggcgaa taccgtgtgg tcgtcaacaa tcagtacggc
      661 acggcgatca gccatgtcgc gcgcttgatc gtggacccgg gtgttccggt cctcgtcacc
      721 caaccgcaaa gccagaccaa acgggtgggc gccgaggcga tgttcacggt tgaggccacc
      781 ggggcgctgc cgttgactta ccaatggcac aaggatggcc aggcaatcac cggcgccatc
      841 gaaccgacgt accatgtcgc cgccgtgagc gccgcggatg ccggcgcgta cacggtggtt
      901 gtgcggaatg tctccggctc ggtcatcagc gaggtggcaa cactggttct cgtgttcacc
      961 ccgcccgcgg tgaacgtcga cctggcggtc gcgtccggac agaatgtgta taccggcgtt
     1021 gccgcggctc ccgacccggg aacggtctgg aaccaggtca cggagaacgc cctcagatca
     1081 gcccttgacc gcacgctggc gttggtggat tcggacggcc tgccgtcgtc catcggcttc
     1141 acctccgcga gtttcggcga gcccggcagc ggtctgtttg ccaacaatgg cggcggcaat
     1201 gctctgcagg cgacctattg gcacagcggc gtgggaaacg agagtccgcc atttggcttc
     1261 acgaatctcg atcccgcggt gcggtatgac gtctatgtct acggcatcgc cacggatttt
     1321 ggcctcgggt acacccagcg catcagccgc gtcggaggcg aatcccaggc cctcaaccag
     1381 gtggtgaaca ccggattccc gattcttcac gaggattacg cgctgttccg cgacatcacc
     1441 ggcgtgacgg aggtgatgtt caccgccggc gagtcgggca atggcgccgt gttcagcacc
     1501 gtgaccggtc tgcaaatcat ccccgccacc gacactccga agattctcca atcgccaacc
     1561 gatcgcttcg tgcgccaggg cgccagcgtc acgttcacgg tggtggcgac cggcaccgcg
     1621 ccgttcacgt accagtggcg gaagaacggg gtagcaatcc agggggcgac gggcgacact
     1681 tacgtcattg catcggcccg ggccgacgac gaaggcctct acagcgtggt ggtgagcaac
     1741 gccgccgggt cggtgaccag cgaaagcgga cgcctgactc tgcagacggc gccgccggcg
     1801 gtgaacatca acctgatcgc cgccgccgac agctcggagt atgccgggac cgcggcggct
     1861 ccggatgtcg ggaccgtctg gaatcaggtc accgaggagg ccttgcgcgc ggatgcggac
     1921 cgcgagatgg cgttggtgga ctcggacggt ctaccggtgg acgctcggtt cttttcggga
     1981 agcttcggcg aaccctcgag cacatggttt gcggagaatg gcgggggcaa cttcctgcaa
     2041 cgcagctatt ggcatgtcgg cggaggcaag gcttcgcccg agttcggttt tcgcaacctc
     2101 gaggccggca agacctacga tgtgtatgtg tacggcatcg ccaccgactt caacctcggc
     2161 tggggccagg cctacaccct ggtcggcggc cccacccaga cgctcaagca ggttccagac
     2221 accggttttc ccgtcgtgaa cgaggactat gtcgtgttca agaccatcag cggcgtcgct
     2281 gagatcaagc tgacgtccgg ccaggcgggc gccttcttca gcacggtcgc cggccttcag
     2341 ctcatcgaga gcgccaaagg cgtccccgcc accttgacga tcacgcggtc tgtcacgggc
     2401 gagattgtga ttggctggac aggcagcggc gtgctcgaac aggccgacgc cgtaaccggc
     2461 gcctggaaaa gggtggcaaa tcccgccaac ccgtacactg ctgcgcctgc gggcgccgca
     2521 aggtactatc gcgtcgtgca agactgagag accatgcgca aaaacgcctc cccattgcga
     2581 acgcccgcca tagacggccg gcaatcgaag aacgccctgg cctttcgacc atgggcagcg
     2641 accttgaatc gttcctggag gaaccttccc gcggctgccc gagtgttctt gtatcgagac
     2701 ggcggcagtt gccagtcagg acctatcccg cagccgcggg atcggcccac cgagagaggc
     2761 agtccgtgga aagcgtccct gttgagcctg ggcgccatcc gggttctcgg cgtcattgtc
     2821 ggtctggcga tgttcacgga tgccgcgcgc tgcgccacgg tccagccgcg gtactacggg
     2881 cacgccgccg tctgcgacgc gcacggcgtg attgcgccct ggtatcgggg actcaacggc
     2941 caatgcgact tccgcatccg cgtcgcggcg gagactctga agagataccc gtggaccgat
     3001 accaacagcg ccattgcggc gtatccggac tatttgttca cgagcttgtg gcagatcgac
     3061 ccggacggtc gcatcacgcc caaggcgtct gatgcctgga tgaacggcga cttgggccag
     3121 cgctcgacca gcctgcttaa cggttgggtg gattactacc gctattcggg cgatccggcc
     3181 gcggtcgcgc atctgaccta cctgggcgag ttcttgctgg atcactgcct gacgccgccg
     3241 gatcacccgt ggccaggctt gttcatcagc gttccggcca aaggcaaagc ctacgggaat
     3301 tgcgatccag aggggatgat tcaattggac atctgcgcat caactgggca gggcctgctg
     3361 cgggcctacc aactgaccgg gcgcacccgc tggctggccg cggctcggca ctggggcgat
     3421 ctcctggccg agcgctgtcg gaaggaccca ggcgaggcgc cttggggacg ctatgcgaac
     3481 cccgaggcgg cgccctggaa ggacaatcaa cagactggcg gcgtgacgat gatcctggcg
     3541 ttcctcgacg acctgattcg cctgggttac accggacaag acaatgcgat tgtgtcggca
     3601 cgcgacgccg ggcgggcgta tttgcgcgat cggttgttgc cggcgtggac ggtcgacgat
     3661 acatggggcc gctatttctg ggactgggcc aacccgaccc agaattgcgc gacgacgccc
     3721 gacgccgccc gctatctgct gcaccaccag gccgagttcc caaactggca aaccgatgtc
     3781 cgcaacatcc tcaccctgtt cctcaaccgg agcggcgttg cgccagagtc gaggggcgat
     3841 gtctacagcg gcgcctgggc ctttcccgaa tcgagttcct gttgcggacg ctcgctctgg
     3901 tacagcccat tcctgctcgc taccgccctg gccgagtatg cttgtcacac ccggagcgac
     3961 tgggcccgtg aactggctta ccggatgatg gttctccaga cctacgatgc ccacgaaacc
     4021 ggtgtgaccg aggacaatat cgacggcg
//
LOCUS       DAOQRO010000318         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_24213_length_4029_cov_5.468546, whole genome shotgun sequence.
ACCESSION   DAOQRO010000318 DAOQRO010000000
VERSION     DAOQRO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24213_length_4029_cov_5.468546"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKK20_11075"
     CDS             complement(<1..726)
                     /locus_tag="PKK20_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00472.1"
                     /translation="MNFPRFGTEKGTHAGPRGSHRHVALGALRLLIIAASLATNAGAS
                     ELHIGAASVDITPTLPVALCGQFNLRIATTAETPLTANVLALESREQDRTLDLAIMVS
                     CDLVGIPDEMLKMVRQEVRRQLPEVDTRKLFLNATHTHTAPVLDNAPGSSFLYPIPKD
                     GVLQPEEYVRFFVGRVSGAIVEAWNTRRPGSVTWGLSHAVVACNRRTVYSQRLPTPGA
                     FGDGTARMYGDTRVPEFRNLEGME"
     gene            complement(1074..3236)
                     /locus_tag="PKK20_11080"
     CDS             complement(1074..3236)
                     /locus_tag="PKK20_11080"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNV00473.1"
                     /translation="MRIPCAALCLLGLARLASSQPSASPGEGALRPWTDYRVIMWVGN
                     RAWAQPDKAHLFLDRLREMGIDTVMAYGTDAAAQACLDRQFPYYVENIVNRGLCLKWN
                     SSVTDWDKHVTAWAKNGRPETAFVRDYCLDDPEWLEWARDQMRAAARRHGPNAPLAYD
                     ISDELSVTVSANPFDYDFAPAALAGFRVWLRTQYRDLDALNRAWATSFAAWDDVRPFT
                     TDQIKHRMGSGEASPRGSPDWQALQRLDVRTLAFLKEPVRWNLAPWCDFRTYMDLCLE
                     RTLDALRRAVRAIDPETPVGIEGTQMPHAFGGYDLARLARALDWVEPYDIGNAREIFG
                     SFMPGRPILATVFEKETNPARRRLWHLLLEGDRGCIIWWSEDCIDWDRGEYPLTPKAL
                     ALAPVLREMRSSLARLFLEAERERDPIYIHYSQPSIQVNWLLESTVDGSTWHRRFSSF
                     EASHNRQAKVRNAWLKALQDLGWSPQFIAADRLGRFEPDPATDCTLVLPQSWAMSDPE
                     IEAVRRLVHEQPPRARPALRRTVLCSGAPGLFDQHGRLLASHRLDGLFPGTIDAKPSA
                     RHANASALRGLDLDLAAFPAERLKRVPDPALWRWLHEQIGCGPIRVPVEARARVHRFR
                     IGDVRLVAIERNIDYHMSENLEQAGGTYALEQPIELTAELTGSAGPLHVYDLRAERHL
                     GCLSRWTFTLDPWSPSLFALLEEPRASEGLIEALLESR"
BASE COUNT          723 a         1213 c         1403 g          690 t
ORIGIN      
        1 ctccatcccc tccaggtttc tgaactcagg gactctggtg tctccgtaca tcctagctgt
       61 tccgtcaccg aaagcgcccg gcgtcgggag cctttgtgaa tagacggttc gccggttgca
      121 ggcgaccacc gcgtggctca acccccacgt cacgcttcca gggcggcgcg tgttccatgc
      181 ctcgacgatc gctccggaga cgcgccccac gaagaaccgg acgtattcct caggttgaag
      241 gacaccgtct ttcgggatcg gatataggaa tgaagaaccc ggggcattgt cgaggactgg
      301 agccgtgtga gtgtgagtgg cattcaggaa gagctttcga gtgtccactt caggcagttg
      361 ccggcgcacc tcctggcgga ccatcttgag catctcgtcc gggatgccga ccagatcgca
      421 ggagaccata atggcgaggt ccagcgtgcg atcttgttct ctggattcca atgccaggac
      481 gttggcggtc aacggagtct ccgcggttgt cgcgatccgc agattgaact ggccgcagag
      541 ggccacgggc agtgtcgggg tgatatcgac agaagcggct ccgatgtgca attcggaggc
      601 gcctgcgttt gtggccaagg acgcggcgat gatcaggagt cgaagcgctc ctagcgcgac
      661 gtgcctatga gagccacgcg gtcccgcgtg cgtccccttt tcggtcccga atcttgggaa
      721 gttcatggtc ggcaccgtaa ccgacggaac cgccactgcc aagcttaaga tcccaggcgc
      781 aagcccaggc agtgcatctg gaggttgtcg gtgggaatcg agtatgcgcc gcgtggggcc
      841 tgcttgccgc gcctgcctgt gccgtgcccg acacggcaga caggccgctg cgctttgcag
      901 cgcaggcagg caggcgtcgc ccctccggca gtctgcatcc gttgtaggcc cggcaccatc
      961 accgggcgac agaataccga caactgtcaa atgcaccgaa gcccagacgg cagcacggat
     1021 tgagccgcgg ggacacggcg gagaaagacc agcgccgcct gtcgccctgg cggtcaacgg
     1081 gactcgagca gcgcctcgat caggccttcg gacgcccggg gttcctcgag cagagcgaac
     1141 agggaggggc tccagggatc gagcgtgaag gtccagcggc tgaggcaacc gagatggcgt
     1201 tcggcgcgca ggtcgtagac atgcaacggg ccggctgagc cggtcaattc ggctgtgagt
     1261 tcgatcggtt gttcgagcgc gtaggtgccg cccgcctgct cgagattctc gctcatgtgg
     1321 taatcgatgt tgcgctcgat ggcgaccagc cggacatcgc cgatcctgaa gcgatgcaca
     1381 cgcgcccggg cctcgaccgg cactctgatc gggccgcacc cgatctgttc atgcagccag
     1441 cgccacaggg cgggatcggg gacgcgcttg agccgttccg cggggaacgc ggccagatcg
     1501 aggtcgaggc ctcgaagcgc cgacgcgttg gcatggcggg cggacggttt agcatcgatc
     1561 gttccgggaa agagcccgtc gagccgatgg gaggccagga gccgcccgtg ttggtcgaag
     1621 aggccgggcg cgcccgagca caacaccgtg cggcgcaggg ccgggcgcgc gcgcggcggc
     1681 tgttcgtgga cgaggcggcg gacggcctcg atctcgggat cggacatggc ccacgactgg
     1741 ggaaggacga gggtgcagtc ggttgccggg tcgggttcga atcgtccgag ccgatcggcg
     1801 gcgatgaact gggggctcca gccaaggtct tgcagagcct tgagccacgc gttgcggact
     1861 ttggcctgtc ggttgtgact ggcctcgaaa ctcgagaacc gccggtgcca ggtcgagccg
     1921 tccactgtcg attcgagcag ccagttgacc tggatgctgg gctgagagta atggatgtag
     1981 atcgggtccc gctcgcgctc ggcctcgaga aacaaccgag ccaaagaaga ccgcatttcc
     2041 cggagcacag gggccagggc cagggccttg ggcgtgagcg gatactctcc gcggtcccaa
     2101 tcgatgcaat cttcgctcca ccagatgatg catccgcggt ccccttcgag gagcagatgc
     2161 cagaggcgcc ggcgagccgg gttggtctcc ttctcgaaaa ccgtcgccag gatcgggcgg
     2221 cccggcatga acgaaccgaa gatctcgcgc gcgttgccga tgtcgtaggg ctcgacccaa
     2281 tcgagcgcgc gggccaggcg cgcgaggtca tagccgccga aggcatgggg catctgagtg
     2341 ccttcgatgc cgacgggcgt ctccggatca atggcgcgca ccgcccggcg tagagcgtcg
     2401 agcgtccgtt cgaggcagag gtccatgtag gtgcggaagt cgcaccaagg cgccagattc
     2461 cagcggacgg gttccttgag gaacgcgagg gttcggacat ccaggcgctg cagggcctgc
     2521 caatccggac tgccgcgtgg cgaggcctct ccgcttccca tgcgatgctt gatctggtcg
     2581 gtggtaaagg gccgcacgtc atcccaggcg gcgaacgacg ttgcccaggc gcggttgaga
     2641 gcatcgaggt cgcggtactg tgtcctgagc cagacgcgaa agccggccag ggctgccggg
     2701 gcgaagtcgt aatcgaacgg attcgccgag acggtcaccg agagttcgtc gctgatgtcg
     2761 taggcgagag gcgcatttgg gccgtgccgc cgggccgccg cacgcatctg gtcgcgcgcc
     2821 cactcgagcc actccggatc gtcgagacag taatcgcgga cgaacgcggt ctcaggtcgc
     2881 ccgttctttg cccaagcggt gacgtgtttg tcccagtccg tcacacttga gttccatttc
     2941 aggcagaggc cgcggttgac gatgttctcg acgtagtagg ggaactgtcg atcgaggcaa
     3001 gcctgcgccg cggcgtccgt gccgtaggcc atgacggtgt cgatgcccat ctcgcgcagc
     3061 cgatcgagga acagatgcgc tttgtcgggc tgcgcccagg cgcggttgcc aacccacatg
     3121 atgacacggt aatcggtcca cggacgcagc gcgccctcac cgggggatgc ggacggctgg
     3181 gagctggcga gccgagccaa ccccaggagg catagtgcag cgcagggaat tcgcacgcgc
     3241 cgagcgtgcc gccgcggcgg cgtcgagtca acccgctcgg ccggactcgg agggccgagt
     3301 tccaagaggc cctggtcggt gacggtgatt gtctcgagac ggtgcccctc ctgcgactgc
     3361 gggatcggcc ctgcggcatt tggttgcggc tttgccgcgc taagggtctg tgctgggctt
     3421 ccaaagcggc gcctcggagc cggcgagatc ggcgatcacg cgggctcgcg ggtcgtcata
     3481 aggcgccgcg gacatcaggg cctcgatgcg gagggtggcc ggctgcgcgt cgaacggatt
     3541 ggagtcctgc cactcgagcg accacggcgc gtcgggcgcg gcaatgtggg caggggaacg
     3601 ggcgcggcga aagcggggtg ataaagtgac aggttcttta tttctgattc agggcggaac
     3661 acagattgac gccgagatgg ataaagacaa ggtgacaggt tctttatctg gaaggcatgc
     3721 acgccgcgct gtccagtgct ctttgggcct catctcctcc gtgcagcgcg gggtgccgtc
     3781 tttgggcgaa cagggctaaa gccgcgaaac ggacgccaac gacagccagg gaactggcgg
     3841 cgctgaagga tgtccatggg gagcgttgac attcaacgat ttaatgcgcc ttgcccacag
     3901 aaacctgaaa cgttcaaacg cccaattctc gactctcgac tctcgactct caacgcctca
     3961 ggtcactgtt cctggcagcc tgtcggactt agatcgagaa gcaagtgagg gtgatggtgg
     4021 taggagtcg
//
LOCUS       DAOQRO010000319         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_24639_length_3984_cov_4.019343, whole genome shotgun sequence.
ACCESSION   DAOQRO010000319 DAOQRO010000000
VERSION     DAOQRO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24639_length_3984_cov_4.019343"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /gene="hypF"
                     /locus_tag="PKK20_11085"
     CDS             <1..1818
                     /gene="hypF"
                     /locus_tag="PKK20_11085"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNV00474.1"
                     /translation="LWRPDASVIHTHHDALIEAARAVRAGAVVAVKILGGFHLVVLAA
                     DDLAVCRLRDRKGREAKPFALLFQSADQVRQVCAVSPLEDRLLRSAEAPIVLLRRHRT
                     CSRGEAEVAPAVAPDNPCLGVMLPNTPLHHLLMAEVGGPVVATSGNRSDEPLCTDERE
                     ALDRLGGIADLFLVHNRPIVRQVDDSIVRVVLDRELVLRRARGYAPLPIAVRDLMPPV
                     LAVGAHLKNTAALARGRHVFLSQHIGDLETPAAHTAFQRVVRDLPALFNVRPALYAAD
                     AHPDYLSTQWARASGRRVLAVQHHYAHALACMAENDVDPPLLGVSWDGTGLGIDQTIW
                     GGEFLRITAHGFERAGHLRCFRLPGGDKAAQEPRRAAIGLLEALLGDAAFANTNLASV
                     AAFTATELKGIRQMLERQVNTPATSSAGRLFDAVASLLGLRQKAGFEGQAALDLEFAL
                     ENAQTDAAYPIHIRHSTALVSHAAPAPGAGCAPPGAIHGVADTSKDPPPQFVLDWAPM
                     IQGILDDLRLQTPACEISARFHNTLAEGILAAARFTGERRVVLSGGCFQNRYLLEQTV
                     RRLRVEGFSPYWHQRVPTNDGGIALGQVMAVARARTKRS"
     gene            1829..2062
                     /locus_tag="PKK20_11090"
                     /pseudo
     CDS             1829..2062
                     /locus_tag="PKK20_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
     gene            complement(2103..3233)
                     /gene="mqnE"
                     /locus_tag="PKK20_11095"
     CDS             complement(2103..3233)
                     /gene="mqnE"
                     /locus_tag="PKK20_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNV00475.1"
                     /translation="MMHGLIERSEWRDLEDKVEAGARLCDKDARRLFASSDLNSMGVL
                     ANRAAERKVGRRASYILNRYINYSNYCILSCQFCAFARKKRDADGFELSIEQMVEKAR
                     KALALGITELHIVGGLHPALPFDYYTGLLRALKALDSRLQLKCFTAIEVLHLSRLSGQ
                     SVRDTLVALKKAGMDSLTGGGAEIFSPTVRQAIARGKETAEEWADVHRTWHRLGGRST
                     CTMLFGHVESASDRVDHLRRLRALQDETHGFTGFIPLPYQPENNAIPVRHAPTGFDAL
                     RTIAVSRLYLDNIDHITAYWVGMGLKLAQVALNHGADDLHGTIVEEHIFHMAGARSPQ
                     VQTEADMVRAIRDAGRIPVRRNTFYEPIRVWEEEGQSALPAS"
     gene            complement(3230..>3984)
                     /locus_tag="PKK20_11100"
     CDS             complement(3230..>3984)
                     /locus_tag="PKK20_11100"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNV00476.1"
                     /translation="LVARCAEAPPQVEWTARDGVGHAAWVIEDGRDLEFIERTFARMP
                     CLYIADGHHRTAAAARVCRVRGGRGGSGHFLAVIFPHNQLQILAYHRVVKDLNGHTPA
                     TLWTRFESIFEPAPGLPDQPGAKHEVSLYMAGHWRKLRFRQRFIVGADPVDRLDVTLL
                     QRHVLEPVLAIGDPRTSDRIGFVGGVRGTAELERLVDCGSSACAFALFPTVIEDLMVI
                     ADRGETMPPKSTWFEPKLRDGLFSHLLGQDEA"
BASE COUNT          705 a         1454 c         1146 g          679 t
ORIGIN      
        1 ctgtggcgtc cggacgcatc cgtcatccac actcaccacg acgcattgat cgaggcggcg
       61 cgcgccgtgc gggcaggcgc cgtcgtagcc gtcaagatcc tcggcggatt ccacctagtt
      121 gtcctggccg cggacgatct ggccgtctgc cgcctgcgcg accgcaaggg gcgcgaggcc
      181 aaaccgttcg ccctcctctt tcagtccgcc gatcaggtgc gccaggtctg cgccgtgtca
      241 cccctcgaag accgccttct ccggtcggcc gaggcgccca tcgtcctcct gcgccgacac
      301 cgcacatgca gcagaggcga ggccgaggtg gcgccggcgg tggcgcccga caacccttgc
      361 ctgggcgtga tgctgcccaa cacccctctg caccacctgc tcatggcgga agtgggcgga
      421 cccgtcgtcg ccacgagcgg caatcggtcc gacgaaccgc tctgcaccga cgaacgggaa
      481 gcgctcgatc ggctgggcgg catcgctgac ctcttcctgg tccacaatcg tccaatcgtc
      541 cgccaggtcg atgactcgat cgtccgcgtc gtcctcgatc gtgagttggt gctgcgccgg
      601 gcccgcggtt acgccccatt gcccatcgcc gtccgcgatc tgatgccgcc cgtcctcgcc
      661 gtcggtgccc acctgaaaaa caccgccgcc ctggcccgcg gacgccacgt cttcctgagc
      721 cagcacatcg gcgatctcga gacccccgca gcccacaccg ccttccagcg cgtcgtccgc
      781 gacctccccg cactcttcaa cgtccgcccc gctctctacg cggccgatgc ccatcccgac
      841 tacctctcca cccagtgggc acgggcctcc ggacgccgcg tgctcgccgt gcaacaccat
      901 tacgcccatg ccctcgcctg catggccgag aatgacgtcg acccgccgct gctcggcgtt
      961 tcctgggatg gcacgggact gggaatcgac cagacaatct ggggcggtga attcctgcgc
     1021 atcaccgcgc acggattcga acgggcaggg cacctgcgct gctttcgtct tccgggcggc
     1081 gacaaggccg cccaagagcc gcggcgcgcc gcgatcggtc tcctcgaggc gctgctgggc
     1141 gatgcggcgt tcgccaacac taacctcgca tcggtcgccg ccttcacggc aaccgagttg
     1201 aaggggattc gccagatgct cgagcggcag gtcaacacgc ccgcaacctc gagtgccggc
     1261 cggcttttcg acgccgtcgc ctctctcctc ggcctccgtc aaaaagccgg cttcgaaggc
     1321 caggccgccc tcgatctcga attcgctctc gaaaacgcac agaccgacgc cgcctacccg
     1381 atccacatcc ggcactccac agcgctcgtc tcccatgccg cccccgcccc cggcgcgggt
     1441 tgcgccccgc ccggcgcaat ccatggcgtc gccgacacat cgaaggaccc gcccccccag
     1501 tttgtgttgg attgggcccc gatgatccaa ggcatcctcg acgatctccg tcttcagacg
     1561 cccgcatgcg aaatctccgc caggttccac aacaccctcg ccgaaggcat cctcgccgcg
     1621 gcacgcttca ccggagaacg ccgcgtggtc ctctccggcg gctgcttcca gaaccgctac
     1681 ctgctcgaac aaacggtccg gcgcttgcgc gtcgagggtt ttagcccata ctggcatcaa
     1741 cgcgttccga caaacgacgg cggaatcgcc ctcggccagg tgatggccgt cgcgcgcgcg
     1801 cgaacgaaac gaagttgaag ctttgactat gtgtctcgca gttccaggac gagtgatcag
     1861 catcgcaacc gacggtgacg acctgtcccg cagcggcaag gtcagctttg gaggcgtcat
     1921 caaggaggtc aacctcgcct tcgttcccga agtcaaggtc ggcgattacg tcgtcgtgca
     1981 cgtcggcttc gccttgagcg tcgtggacga agccgaggca aacaaggttt tcgattacct
     2041 gcgccagatg gatgagttga cggacctcca gcccggtgag aagcccgcag aggaacccct
     2101 ggctacgacg ccggcagagc cgattgcccc tcctcttccc aaaccctgat cggctcgtaa
     2161 aaggtgttgc gacgcacggg aattcgcccc gcgtcgcgaa tggccctcac catgtccgcc
     2221 tccgtctgca cctgcggcga ccgcgcgcct gccatgtgga aaatgtgctc ctcgacgatt
     2281 gtgccgtgca ggtcgtcagc cccatggttg agtgcaactt gcgccagctt cagccccatc
     2341 cccacccaat acgccgtgat gtgatcgatg ttgtccagat acagccggct cacggcaatc
     2401 gtccgcaacg catcgaatcc ggtcggcgcg tgccgcacag ggatggcgtt gttctcgggt
     2461 tgatacggca ggggaatgaa gccggtgaag ccgtgggttt catcctgcaa tgcccgcagg
     2521 cgccgcagat gatccacccg gtcggacgcg gactcgacat gcccgaacaa catcgtgcaa
     2581 gtgctgcgcc ccccaagccg atgccacgtc cgatgcacat ccgcccattc ctccgccgtc
     2641 tccttgccgc gcgcaatggc ttgccgcacg gtcggactga agatctcggc gccccctcct
     2701 gtgagcgaat ccatgccggc cttcttgagg gccacaagcg tgtcccgcac cgattgccct
     2761 gagagccggc tcaggtgcag aacctcgatc gccgtgaaac atttgagctg caaccgggaa
     2821 tcgagcgcct tcaacgcccg cagcaggccc gtatagtaat cgaacggcaa cgcgggatgc
     2881 agccccccca cgatgtgcag ctcagtaatg ccgagcgcca gggctttccg agccttctcg
     2941 accatctgct cgatcgacaa ctcgaaaccg tccgcatccc gcttcttccg ggcaaacgcg
     3001 cagaactggc aactcaggat gcagtagttc gagtagttga tgtaccggtt gagaatgtaa
     3061 ctggcgcggc gaccgacttt ccgctcggct gcacggttcg ccaacacgcc catgctattc
     3121 aaatcgctgc tggcaaacag acgtctcgcg tccttgtcgc acagcctcgc gccggcctcg
     3181 accttgtctt cgaggtcgcg ccactcgctc cgctcgatca agccatgcat catgcctcat
     3241 cctgtccaag cagatggctg aaaaggccgt cgcgcagctt cggctcgaac catgtgctct
     3301 tgggcggcat cgtttcgccc cgatccgcaa tgaccatcag gtcctcaatc accgtcggaa
     3361 acaaggcgaa ggcacacgcg ctcgaaccgc agtcgaccaa tcgctccagc tccgccgtcc
     3421 cgcgcacccc ccccacgaat ccaatccgat cgcttgtccg cgggtcgcca atggccaaca
     3481 ccggctcgag tacatgccgc tgcaacaacg tcacgtccag acgatccacc gggtccgcac
     3541 ccacgatgaa ccgctgacgg aaccgcaact tgcgccaatg cccggccatg tagaggctca
     3601 cctcgtgttt cgcgccgggc tggtccggca agccgggcgc gggctcgaag atcgactcga
     3661 atcgggtcca gagcgttgcg ggcgtatggc cgttgaggtc tttcacaacc cgatgatagg
     3721 ccagaatctg gagctggttg tgcgggaaga tcaccgccag aaaatgaccg ctccctcccc
     3781 gtccaccccg gacgcgacag actcgggcgg cggcggcggt ccgatgatgg ccgtcggcga
     3841 tgtacaaaca cggcatgcgc gcgaaggtcc gttcgatgaa ctcgagatca cgcccgtcct
     3901 cgatgaccca cgcggcgtgg cccacaccgt cacgtgcggt ccactcgacc tgtggaggcg
     3961 cttcagcaca gcgcgcgacc aggc
//
LOCUS       DAOQRO010000320         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_24708_length_3975_cov_5.150000, whole genome shotgun sequence.
ACCESSION   DAOQRO010000320 DAOQRO010000000
VERSION     DAOQRO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24708_length_3975_cov_5.150000"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..379
                     /locus_tag="PKK20_11105"
     CDS             107..379
                     /locus_tag="PKK20_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00477.1"
                     /translation="MNCVSIIMHASRNPRPATASYTTFELLSGIIETAINEEMRTFDL
                     YLTDLLAAGVVMRQTALEYAVNRHRLEMALRGFASATPILRRDEDR"
     gene            469..1176
                     /locus_tag="PKK20_11110"
     CDS             469..1176
                     /locus_tag="PKK20_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00478.1"
                     /translation="MLTLLRIVLTVALLYGLMQAAKNAGENPQAGDLANAFWMAYCVI
                     IGIFAAITWAPVVGSRIAEPLTGILTDSTSVERPNWLMKLIRWLERKRCRFLVRWFCF
                     AEGIRHPWLPAQFITGLNHARPGSWLELVFAREVYRFNNLQNCMQAFRVLRGRGVTPQ
                     PHPNPEINLELMSLEITAKPDDAVLPVPASSTPHLKRNPRIRLAADDPASGPPPPSSP
                     TTSGPADAGSPNSADRP"
     gene            1173..1790
                     /locus_tag="PKK20_11115"
     CDS             1173..1790
                     /locus_tag="PKK20_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNV00479.1"
                     /translation="MKKGLFWTLHRAMKALVWCFWIAAAGFLYTQRATLEPAMDLAEL
                     WWNRPSSRPSELGRIEGRVTRIFAADSLQLRDDNGWLFNYGLAGASVPKTSPNATLND
                     RYAARAGLTNVTRLVLDQRVAIDVTLANPQTRTGLGLVRVGETNVNETLLRDGLARLK
                     RDQIRALPLLDQYALVRAERLARRERIGLWAPTAAEGLSPEPQAQ"
     gene            2001..2339
                     /locus_tag="PKK20_11120"
     CDS             2001..2339
                     /locus_tag="PKK20_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00480.1"
                     /translation="MIYLLDTCALVSAFARLETAFKRWAEALLRTIRLPLATCEPVVT
                     EAAHMLGSGDRLLEAMHRGLLPIGLGASRRRHEHQHAQRGDAGIAKDVAGGYFAPAAT
                     LRRSNPGGRV"
     gene            complement(2285..3817)
                     /locus_tag="PKK20_11125"
     CDS             complement(2285..3817)
                     /locus_tag="PKK20_11125"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNV00481.1"
                     /translation="MKTTLSILAMIALCGGATTLAQLTPGNPLSGLERLKDFEARRAS
                     SSDANWKDGNADARPIAPGGILTLAELEGPGVITHIWFTIAHEAPFYSRLLTLRMYWD
                     GEDHPSVECPVGDFFGIGHGVDKSFISIPIRVSSEGRARNCYWPMPFRKSARLTVTNE
                     SDKRCGAFYYYVDWQKQDSLPDNTAYFHAMYRQEHPCVMGRNYLLADLAGRGHYVGTI
                     QSVYHMSSGWYGEGDDFFFVDGAAEPQLRGTGTEDYFCDAWGFRQQDGPFYGTPLWEG
                     YSEGDRSTAYRFHIPDPVPFAKSLRVEIEHKGSQYFPDGQVSGFIERDDLMSSVALWY
                     QTEPHKPWPALPPGPDRLPFREQTLVVGWKAVPEAKHSEAPLEVQPIGGTTDGKQLWF
                     RPSTGESWLEVTFRTDAEMTAELWVKMLHAPDYGAYRVRLDGRDAGALDLYGPGIAPM
                     PHRLGTFTLASGEHTLRFEGVGKNSQSKGYLLGFDALTARVPVYTRPPGFDLRKVAAG
                     AK"
BASE COUNT          709 a         1266 c         1255 g          745 t
ORIGIN      
        1 ggctgagtgg ttaaaggcac ctgactcgaa atcaggcgta gtcgcaaggc taccgggggt
       61 tcgaatccct ccccttccgc cagtatttgc aaactggctc ccgagagtga attgcgtttc
      121 gatcatcatg cacgccagcc gcaatccccg cccagcgact gcgtcatata caacattcga
      181 actgttgagc ggaatcatcg agactgcgat caatgaagag atgcgcacat tcgacctgta
      241 cctcaccgat ctgctggccg caggcgtcgt catgcggcag acagctctcg aatacgcggt
      301 caaccggcat cggctcgaaa tggccttgcg cggcttcgcg agcgccacac cgatcttgcg
      361 ccgtgacgaa gaccgataga atcctggaat aatgaagcgc gttgctcgca tcacaggttg
      421 ggcgccgctc atggattggg cggaggcgcg tctcgacaca ttgtcgctat gctgaccctt
      481 ttgcgaattg tgctgactgt cgcgctgctg tacggcctga tgcaggcggc caagaacgcg
      541 ggggagaacc cgcaggcagg cgatctcgcc aacgccttct ggatggcgta ttgcgtcatc
      601 atcggcattt tcgccgccat aacctgggcg cccgtggtgg gcagccggat cgccgagcct
      661 ctgacgggca tcctgacgga cagcacctct gtcgaacgcc ccaattggct gatgaagctg
      721 atccgctggc tcgaacgcaa gcgctgtcga ttcctcgtcc ggtggttctg tttcgccgag
      781 ggaatccggc atccctggct cccggcccag ttcatcacgg gtctgaacca cgcgcggccc
      841 ggctcgtggc tcgaactcgt cttcgcgcgc gaggtgtatc ggttcaacaa tcttcagaac
      901 tgcatgcagg cgttccgtgt cttgcgcgga cgcggcgtca cgcctcaacc gcatccgaac
      961 cctgagatca accttgagct gatgtcgctc gaaataaccg ccaaaccgga tgacgcagtc
     1021 ctccccgttc ccgcgtcgtc aacccctcat ctcaaacgca acccgagaat ccggctcgcc
     1081 gccgatgacc cggcatcggg gccgccgcca ccctcgtcgc cgacgacgtc gggtccggcc
     1141 gacgccggct cgcccaactc ggctgaccgc ccatgaaaaa gggcctcttc tggacgttgc
     1201 atcgcgccat gaaggcgctt gtctggtgct tttggatcgc agcggccggc ttcctctaca
     1261 cgcagcgcgc caccctcgaa ccggcgatgg atctggcgga gctttggtgg aaccggcctt
     1321 cgagtcggcc atcagagttg ggccgcatcg aggggcgggt gacccggatt tttgccgccg
     1381 acagtctcca actgcgcgat gacaacggct ggctcttcaa ctacggcctg gcgggagcct
     1441 cggttcccaa aacgagcccg aacgcgactc tgaacgatcg ctacgcggcc agggcgggcc
     1501 tgaccaatgt gactcgcctg gtgctggacc agcgtgtggc catcgacgtc acgctggcca
     1561 atccccagac ccggaccgga ctgggcctgg tgcgggttgg cgagacaaac gtgaacgaga
     1621 cgctgctccg cgacggattg gcgcggctga agcgggacca gattcgcgct ctgccgcttc
     1681 tcgatcagta cgcactggtc cgtgccgaac gcctggcacg gcgcgaacgg atcggccttt
     1741 gggcgcctac cgcagccgaa gggttgtccc ccgagccaca agcccaatag accatcgcgc
     1801 tcccttgact cgacctgcgg tggcggggag gtgatccggc ctcaaccgct cgatcaatcc
     1861 aaaagggctg gtcgcatccc atcaaccgag gatcaactct gccagcagac tcgattcaca
     1921 gtccgcgcgc catgtccttc gcagggcgca gtatatgctg acccgtttaa ggccgtgcgc
     1981 cacgccctgc taagcggacg atgatctacc tcctcgacac ctgcgcgctg gtgagtgcgt
     2041 tcgcccgcct ggaaaccgcg ttcaaacgtt gggccgaggc cctgctgagg accatccgcc
     2101 tcccgttggc gacctgtgag cctgtcgtca ccgaagccgc ccacatgctg gggtcgggcg
     2161 accgtttgct cgaagctatg catcgcggtc tgttaccgat cggtcttggt gcttctcgcc
     2221 gtcggcacga gcatcaacat gcccaacgtg gcgacgccgg gatcgcgaag gatgtcgcgg
     2281 gcggctactt cgccccagcg gccactttgc gcagatcgaa cccgggcggg cgggtgtaga
     2341 ccggcactcg agccgtgagc gcgtcgaaac cgagcagata gcctttggat tggctgtttt
     2401 ttcccacgcc ttcgaaccgg agcgtgtgct cgcctgacgc cagagtgaat gtgcccagcc
     2461 gatgcggcat gggggcgatg cccggaccgt acaggtcgag cgcgcctgcg tccctgccgt
     2521 ccagccgcac tcggtaggcg ccatagtccg gcgcgtgcag catcttgacc cacaattcgg
     2581 cggtcatttc ggcgtcggtt ctgaaggtca cttcgagcca gctttcgccg gtcgagggcc
     2641 ggaaccagag ctgtttccca tccgtcgtcc cgccgatggg ctggacttcg agcggggctt
     2701 cggagtgctt ggcctcgggc acagccttcc aaccgacaac caatgtctgc tcacggaacg
     2761 gcaggcgatc cggacccggc ggcagggcgg gccagggctt gtgcggctcg gtctggtacc
     2821 acagggcgac cgacgacatc aggtcatcgc gttcgatgaa cccgctgacc tggccgtccg
     2881 ggaaatactg cgagcccttg tgctcgatct cgacccgcag cgatttggcg aacgggaccg
     2941 gatccgggat gtggaaccga tacgccgtgc tgcgatcgcc ctcgctgtat ccttcccaaa
     3001 gcggcgtgcc gtagaacgga ccatcctgtt gtcggaaccc ccaggcatcg cagaagtaat
     3061 cttcggtgcc tgtgccgcgc aattgcggct cggccgcgcc atcgacaaag aagaagtcgt
     3121 cgccttcacc ataccagccc gaggacatgt ggtagacgct ctggatggtc ccgacatagt
     3181 ggccgcgccc ggcgaggtcg gccagcaggt agttgcgtcc catgacgcac gggtgctcct
     3241 gtcggtacat cgcgtggaaa taggcggtgt tgtcgggcag ggaatcctgt ttctgccaat
     3301 cgacgtagta gtagaaggcg ccgcatcgct tgtcgctttc gttggtgact gtcaggcggg
     3361 ccgacttgcg gaacggcatc ggccaataac agttgcgcgc ccggccttcg gaggagaccc
     3421 ggatcggaat cgagatgaac gacttgtcga caccgtgccc gattccgaag aagtcgccca
     3481 ccgggcactc gacgctcggg tgatcttcgc cgtcccaata catgcgcagg gtcaggagcc
     3541 gggaatagaa tggggcctca tgcgcgatgg tgaaccagat atgggtgatg acgccggggc
     3601 cttcgagttc ggcgagagtc aggatgccgc cgggcgcgat cggacgggcg tctgcgttgc
     3661 cgtctttcca gttggcatcg gaggaagagg ctcggcgcgc ctcgaagtct ttgagccgtt
     3721 ccagtccgga cagtgggttg ccgggcgtga gttgggccag ggtcgtggcg ccaccgcaca
     3781 gggcgatcat ggcgaggatc gacagagtcg ttttcatggg cttatgcagg cgctcactgt
     3841 gacgcggccc ggggcgtccg acaagacgca attgggtccg ccaatccccg agccccgcta
     3901 ccctgacgat cactttttgt ccagggcggt tctgagctgg gatggggtga ttgaggaaga
     3961 gggacttaac gaatt
//
LOCUS       DAOQRO010000321         3962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_24816_length_3962_cov_4.359099, whole genome shotgun sequence.
ACCESSION   DAOQRO010000321 DAOQRO010000000
VERSION     DAOQRO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24816_length_3962_cov_4.359099"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1255)
                     /locus_tag="PKK20_11130"
     CDS             complement(191..1255)
                     /locus_tag="PKK20_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNV00482.1"
                     /translation="MLGFLAAGIGAQFAKAHGATVRAPGASAEEQAGETPGGEASGGV
                     SSAVSAMEAKAKVFEFLRARGHGSYVFGQVATWVHNENPDMDHPGNWIRKVLEHTGRM
                     PRYGCITYDFDDDPFTDAAWNEGARKLWERGMIVGIYNFFANPSGGRWNDPCEMEKIW
                     SPDDNPVRRNFFRQLDRMAANLQWLKDRGIPVVYTPFVESDDRNKWHAKGGSDAIIRL
                     YRLVHDYFQGTKKLDHILWAYHTTQNHGALERCYPGDDYVDVLGKSAYGTGLVFSEYE
                     WAVAKKKTGGKVIWWAELGIRGKGDPARDCLDVLRKLESSYPELAGFVFWSDEGFYNV
                     VGNLNGREFMEHPKIVTLGK"
     gene            complement(1267..1794)
                     /locus_tag="PKK20_11135"
     CDS             complement(1267..1794)
                     /locus_tag="PKK20_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00483.1"
                     /translation="MRRIDSSRRVLGSVARLRTDGRDRVRAYNDTVVLLDGKPFVSAE
                     GGTCGRSQAMRTALSALFAFNSRTNDAGLQTHWGLDGRARFLLKGKPERKVLHTVQVG
                     RNGFLHALAVGTSDENRGCEYLGASVHALDFVLSRHSESRRIPACSTRQCQPIKLFAS
                     HSFCGHRWVRSGRAR"
     gene            complement(2304..3353)
                     /locus_tag="PKK20_11140"
     CDS             complement(2304..3353)
                     /locus_tag="PKK20_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNV00484.1"
                     /translation="MKASSIILTALCVTIALRAQSDDLDPRNWIGHDRTRPLPPVVDP
                     GVGSTPDQAGKAPSDAIVLFDGTNLSKWVAMNGEPTKWIVKDGAMECVPGSGYVRTLQ
                     CFGDAQVHVEFASPAKPEGSGQGRGNSGFFFGGMRYEIQVLDSYQNDTYADGSCASIY
                     NQYPPLVNASRPPGQWQSYDILWTAPRFESDGKLRSPARVTLFHNGVLVHHNVELIGD
                     TGWVTRGPYRAHPEKLPLAIQDHGNPVRFRNIWVRELGRPGRPEFFLADKVLDGYVGK
                     YQVNRNWIVDVTRRDGCLFFQWAGEDFLVFATSPNRFFAKTTDVQVEFKTENNEDVAY
                     FSVGEDGGMRAKKVK"
     gene            complement(3573..>3962)
                     /locus_tag="PKK20_11145"
     CDS             complement(3573..>3962)
                     /locus_tag="PKK20_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00485.1"
                     /translation="VLHLIDGLFSGAHPRDAVPQRMEMPPFLGHWPCSLLASQDPVAI
                     DSVGFDFLWSERTDFPRKGGVDDYLHEAALAADPPSGTFYDPNHGTPTRRLASLGVHE
                     HWNNPEEKKYSRNLGTGEGIELVKADA"
BASE COUNT          734 a         1348 c         1134 g          746 t
ORIGIN      
        1 cagcgccgac tcacgtcggc ggctacaaat cggttcatgg tccccgtgcg cattacgtct
       61 ttgaatgtca acgctctcca tgaaccgaac gttggagggg gagacggctc gtcccccgcc
      121 gaagagtgtt tttgaaggag tttggggcga cgaggccgtc gcccctccgc aacatgccgg
      181 cgcggtcact tcacttcccc agagtcacaa tcttcggatg ctccatgaac tcgcgtccgt
      241 tgagattccc aaccacgttg tagaagccct cgtcactcca gaagacgaat ccagccagtt
      301 cagggtagct gctctcgagc ttgcgcagca catccagaca atcgcgcgca ggatcgccct
      361 tcccgcgaat ccccaactcg gcccaccaga tgaccttgcc cccggtcttt ttcttcgcaa
      421 ccgcccactc gtattctgaa aacaccaacc ccgtgccgta agccgatttg ccaagcacat
      481 ccacatagtc gtcgcccgga taacaccgct ccaaggcgcc atgattctgc gtcgtgtgat
      541 aggcccagag aatgtgatcg agttttttcg tgccctggaa atagtcgtgc accagccgat
      601 agagccggat gatcgcgtcg ctccccccct tggcgtgcca cttgttccgg tcgtcgctct
      661 ccacaaaagg cgtgtacaca accggaatcc cgcggtcctt cagccactgc aaattcgccg
      721 ccatccgatc gagctgccga aagaaattgc gccggaccgg attgtcgtca ggcgaccaaa
      781 tcttctccat ctcgcacgga tcgttccacc tgcctccgct cgggttcgca aagaagttgt
      841 agattccgac aatcatgccc cgctcccaga gcttgcgcgc gccctcgttc cacgcggcgt
      901 cagtgaacgg gtcatcgtca aagtcgtacg tgatgcagcc gtaccgcggc attctgccgg
      961 tgtgctcgag caccttccga atccagttgc ccgggtgatc catatccgga ttctcgttgt
     1021 gcacccacgt ggccacctgc ccaaacacat aggacccatg accccgcgcc ctgagaaact
     1081 cgaacacctt cgccttcgcc tccatggcac tgacagcgct cgagacgccg ccggatgctt
     1141 caccccccgg cgtctcgccc gcctgctcct cggcactcgc gccgggtgca cgcaccgtcg
     1201 caccgtgcgc tttggcgaat tgcgcgccaa ttccggcggc gagaaacccg agcatctcac
     1261 gccgggtcat cgcgcccttc ctgaccgcac ccatcgatgc ccgcaaaacg aatgactggc
     1321 gaacagtttg atcggctggc attggcgtgt cgaacatgca ggaatcctgc gtgactcgga
     1381 atggcggctc aagacgaaat ccagcgcgtg tacgctcgct cctaagtact cgcagccacg
     1441 gttctcgtcc gacgtcccga ccgccagggc atgaaggaaa ccgttgcgcc cgacctggac
     1501 ggtgtgaagg actttgcgtt ccggctttcc cttgagcaag aaccgggcgc gcccgtccag
     1561 cccccaatgc gtttgaagac cggcgtcgtt ggtccggctg ttgaacgcga acaacgcgct
     1621 cagagccgtc cgcattgcct ggctgcgtcc acatgtccct ccctcggcac tcacaaacgg
     1681 cttcccatcg agcagcacga cggtgtcgtt gtaggctcga acacgatctc ggccgtccgt
     1741 ccgcaggcgc gcgacactcc ccaggacccg acggctggaa tcgatccgtc gcatagcgcg
     1801 gctgcgccac cacaccttcg tcgctgaaca accccgaaag acacggatcc gacagcaccc
     1861 cagccacggt cgtggtggaa ccgttcaccc gcatggcttg atgcaccaac cgcacgccgt
     1921 ggctgtaatc cacccaactc acgccgtggc caaggtagag cggctggatc ggcaccccgc
     1981 cgattcgatg ccatccgtaa atcgcaacct tgccgggcgc ctcatcgagc ctggccgaga
     2041 tcacgacgtc cttcttgtgg cccgcaacaa gcgccccggg cggatgctcc gccatgcgct
     2101 cgagccgctg ggtgcgcaca atcccgttgt gcgctataaa caccggcacc gtcgtcatgg
     2161 ccgggctcgg agcaatcggg gccggctcga gcttgacctc tgcagcccgg taaaccgcgt
     2221 ccaccatcct ccgcgttgga agcatgcagc cgagttggtc tcatggagaa acgtcttcaa
     2281 caccgattgg tctctaatcc gggtcacttc actttcttgg cccgcattcc cccgtcctcg
     2341 ccgaccgaga agtaggcaac gtcctcgttg ttttccgttt tgaactcgac ctgaacgtct
     2401 gtggtctttg cgaagaaccg attgggggac gtggcaaaga ccaggaaatc ctctcccgcc
     2461 cattggaaga acaaacaccc gtcgcggcgc gtgacgtcga cgatccagtt cctgttcacc
     2521 tgatacttcc ccacgtatcc atcgagcacc ttgtcggcca ggaagaactc cgggcgccca
     2581 ggccggccca actcccgcac ccagatgttg cggaagcgca ctgggttgcc gtgatcctga
     2641 atcgcgagcg gcagcttctc cggatgcgcc cggtacgggc cgcgcgtgac ccagcccgtg
     2701 tcgccgatca actcgacgtt atgatgcacc agcacgccgt tgtggaacag cgtcacccgg
     2761 gccggcgacc gcagcttgcc gtcgctctcg aaacggggcg cggtccagag gatatcgtag
     2821 ctctgccact ggcccggcgg acgcgaggcg ttcacaagcg gcgggtactg attgtaaatc
     2881 gaagcgcacg agccgtcggc gtacgtgtcg ttctggtagc tgtccagcac ctgaatctcg
     2941 tagcgcatgc cgccgaagaa gaacccgctg ttgccccggc cctggcccga gccctcgggt
     3001 ttggcggggc tggcgaattc cacgtgcacc tgggcgtcgc cgaaacactg gagggtccgc
     3061 acatacccgc tgcctggcac acactccatc gcgccatctt tcacgatcca cttggtcggc
     3121 tccccgttca tcgccaccca tttcgagaga ttcgtgccgt caaagaggac gatggcgtcc
     3181 gatggcgcct tgccggcctg gtccggcgtg ctgccaacac cggggtccac caccggcggc
     3241 agtgggcgtg tgcggtcgtg cccaatccag ttgcgcgggt caaggtcgtc cgactgcgct
     3301 cgaagcgcga tggtcacaca cagtgcggtc agaatgatag atgatgcttt catacgagag
     3361 tccggctgtc gccatacggc ggatgcttga ccggagagtg aatcagtatc caccggggaa
     3421 atcaacctct ggttcgaagt gtgagcgcgg gccgatgcat ctgaaggttg tcggtgggaa
     3481 tcatccgttg taggcccggc gccagagtga tcgaggcaac ggttcgccct ggagggatgc
     3541 gcccccattc tgtttgcctg caacccgctg tgtcaggcgt cggccttgac cagttcgatg
     3601 ccctcgcccg tgccaaggtt gcgcgagtac ttcttctcct cgggattgtt ccagtgctca
     3661 tggacgccca ggctcgccaa ccgccgggtc ggggtgccgt ggttcgggtc gtagaacgtc
     3721 cccgaggggg ggtcagccgc gagcgctgcc tcgtgcagat aatcatccac cccgcctttg
     3781 cgcgggaaat ccgtgcgttc gctccacagg aaatcgaagc cgacggaatc gatggccacc
     3841 ggatcctgcg atgccagaag gctgcacggc cagtgcccga ggaacggcgg catctccatg
     3901 cgctgcggga ccgcgtcgcg aggatgcgcc ccggagaaca ggccgtcgat gaggtgaagc
     3961 ac
//
LOCUS       DAOQRO010000322         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_25276_length_3918_cov_4.376650, whole genome shotgun sequence.
ACCESSION   DAOQRO010000322 DAOQRO010000000
VERSION     DAOQRO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25276_length_3918_cov_4.376650"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2500)
                     /locus_tag="PKK20_11150"
     CDS             complement(83..2500)
                     /locus_tag="PKK20_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNV00486.1"
                     /translation="MHLKSLSALVALVASLAHAGIIESGEYADAKAAQAAWAPMAGSP
                     PPRVATIEGHKVLHFPCNFQGTTHERASWDRKVKLDLSACRGVQFKLWCGDPSPVSYF
                     SLYFQSGGGWYHAAFFPETTNGWNVITIDKSATTSEGEPAGWGRISAIRFSAWRGQDR
                     NTDLYFSDLRETGVLGDDALIAVVRGESIARVSSGEARSVERFTEAVAQALQASDLGC
                     AVVSDLDVTAERLRGVKLVILPHNPSLPDRAADELIAYVRSGGKLLVFYVVPGALRPV
                     LGVEGGQHISAPRPGHFSAMRFEKGALPGAPPVVGQKSWNISVSKPVPGSGRVLAEWI
                     DDQDQPTGHAAVVGSANGLVMTHVMLPDDAANKRRMLLAMAGHLVPELWRRAVEAGLE
                     RLGTIGEFRSYDEAIQGIRRLAGGNARTVEALAETEQSREVSRKRAAEGRYAEALDAV
                     VLASEKLERAYCLAQRSLPGEFRAFWCHSAFGVDGIAWDEAIRRLATNGFTAVVPNML
                     WGGVAFYQSAILPVAPSVAQRGDQIAECVAAARKHGVQVHVWKVNWNLGHAAPKEFVD
                     QIRTAGRLQSSREGKEESWLCPSHPDNQKLEIESMLEVARRYDVDGLHFDYIRYPDSE
                     HCFCAGCRQRFEQAARTTLGNWPADVLARSPLHEQWLEWRRGHITAVVKAVSEQARAI
                     KPKIKISAAVFPRWTTDRASIGQDWKLWCERGYLDFVCPMNYTPSDRNFENMLAQQVQ
                     WAGSVPCYPGIGVSASSSKFGVDRTIAQIQIARRHRTGGFIIFNYGVPESRDLLPLLG
                     LGITAPD"
     gene            2724..>3918
                     /locus_tag="PKK20_11155"
     CDS             2724..>3918
                     /locus_tag="PKK20_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNV00487.1"
                     /translation="MNTEQLYQERFNRYVTALRNEKPDRVPIRPFVAEFTAKYAGYTC
                     QDVAHDYPKAFEAAIKCAKDFDWDAVVPNMVYVWTGLAQAMGLRYYGIPGIGIPHTVG
                     FNYIEPPEDKAFMKADEYDALIDDPTGFLYNVWFPRVSTEANRIGEPSTYRNNLAFVK
                     GAMAMLAYFYAFGPQTARLRTECGTASAIAGIFKAPFDIIADKLRGYVGLTLDMQTQP
                     KKVLQACEALMPHLCHIGLATSDPNKQVPIGFWMHRGCVPLVNPAQYASHYWPTLKPI
                     IEEFWKNGRQTLFYAEGKWHHHFDSFRELPERSIVFHCDRDDIFEAHRRLHDKFCLSG
                     GIPNGLLSYGKPDEVRAFCTRVLEEVAAEGGYIMDAGAIMQDDTSIENVRVMTDVCRE
                     KGIYSA"
BASE COUNT          732 a         1235 c         1223 g          728 t
ORIGIN      
        1 gattccaaca catgtccaat gccggtctcg gcctccacaa tcccggcttg aatctccaca
       61 tgttcagcct gagttacgcg ttctagtcag gtgctgttat ccccagaccc agcaacggca
      121 gcaggtcgcg gctctcgggc acgccatagt tgaagatgat gaatccgcct gttcgatgcc
      181 ggcgtgcgat ctggatttgt gcaatcgtgc ggtcgacgcc gaatttggac gacgaggcgc
      241 tgacgccaat cccgggatag caggggaccg agccggccca ttggacctgc tgcgccagca
      301 tgttctcgaa attccggtcg ctcggcgtgt agttcatcgg gcagacgaag tcgagatagc
      361 cccgctcgca ccagagtttc cagtcctgtc cgatgctcgc acggtccgtc gtccagcggg
      421 ggaacacggc tgcggagatt ttgatcttcg gcttgatggc gcgagcctgc tcgctgactg
      481 ccttgacgac ggcggtgatg tggccgcggc gccattcgag ccactgttcg tgcagcgggc
      541 tgcgggcaag cacgtcggcc ggccagttcc cgagcgtggt ccgggcggcc tgctcgaacc
      601 gctgacggca tccggcacag aaacagtgct cgctgtcggg ataccggatg taatcgaaat
      661 ggagcccgtc cacatcgtaa cggcgcgcga cttcgagcat cgactcgatt tccagtttct
      721 ggttgtcagg gtgggatgga cagagccacg attcctcctt tccctcccga ctcgattgca
      781 gccgtccggc cgttcgtatc tggtcgacaa actccttggg cgcggcatgg ccgaggttcc
      841 aattgacctt ccagacgtgg acctggacgc cgtgcttgcg cgccgcggcc acgcactcgg
      901 cgatctggtc gccgcgctgt gcgacagacg gcgcaacggg gagaatcgcg ctttggtaga
      961 acgcgacgcc tccccagagc atgttgggga caaccgccgt aaagccattc gttgccagac
     1021 ggcggatcgc ttcgtcccag gcgatgccat cgaccccgaa ggcgctgtgg caccagaacg
     1081 cccggaactc gcccggcagc gagcgctggg ccaggcaata ggcccgttcg agtttctccg
     1141 aagccagcac gaccgcgtcc aaggcctccg catagcgccc ttcggcggcg cgtttgcgcg
     1201 agacttcgcg cgactgctcc gtttcggcca aagcttcgac cgtgcgcgcg ttccctccgg
     1261 cgaggcgcct gatgccttgg atcgcctcgt cgtacgacct gaattccccg attgttccga
     1321 gacgctcaag gcccgcttca accgcccgtc gccaaagctc agggaccagg tggcctgcca
     1381 tcgccaacag catccggcgt ttgttggccg catcgtcggg caacatcaca tgcgtcatca
     1441 caagcccgtt ggctgagccg acaacggcgg catgaccggt cggctgatcc tggtcgtcga
     1501 tccattcggc aaggacgcgg cccgagcctg gaaccggctt ggaaacgctg atgttccagg
     1561 atttctggcc gacgacgggc ggcgcgcccg gaagggcgcc tttctcaaac cgcatagccg
     1621 agaaatggcc ggggcgggga gcgctgatgt gctggccgcc ttcaactccc aggacaggcc
     1681 gcaacgcccc cggcacgacg tagaagacca ggagcttgcc gccgcttcgg acatacgcga
     1741 tgagttcgtc cgccgcgcgg tccggaagcg aagggttgtg cgggagaatc accagcttga
     1801 ccccgcgcaa ccgttcggcg gtgacatcga ggtcgctgac aacggcgcat ccgagatcgg
     1861 atgcctgcag ggcctgtgcg acggcttcag tgaaccgctc gacgctgcgc gcttctcccg
     1921 acgatactcg tgcgatcgat tcgccccgca cgacggcaat gagggcgtcg tcccccagca
     1981 cgcccgtttc gcgcaggtcg ctgaagtaga gatcggtgtt ccgatcctgc ccgcgccagg
     2041 ccgagaatcg gatggcgctg atccgtcccc agccggcagg ctctccctcg ctggtcgtgg
     2101 cggatttgtc gattgtgatc acattccagc cgttggtggt ctcgggaaag aaagcggcat
     2161 gataccaccc gccgccgctc tggaagtaga ggctgaaata cgagaccggc gaggggtcgc
     2221 cgcaccagag cttgaattgg acgccccgac acgcactcag atcgagtttg actttccgat
     2281 cccaggacgc ccgctcgtgc gtcgtgccct gaaagttgca ggggaaatgc aggaccttgt
     2341 ggccttcgat ggtggcgact cgaggtggag gcgatccggc catgggcgcc caggcggcct
     2401 gcgcggcttt ggcgtcggcg tactcgccgg actcgatgat ccccgcatgc gcgagagacg
     2461 cgaccaaagc gaccagggcg gacagtgatt tcagatgcat aacgcgaaga gtctaccggc
     2521 cagggcgtga gaacgtccag ctttgccggg gcgatgtcat cgagtggctc tcgatatcaa
     2581 ggagtactac aacgcgtcgt ggccgctgtc ggagaatctg ttcctcgcgg ctgtcgcagt
     2641 cgacgaaatt ccacaaactg cgattggagt tgtattccag ttgcaggccg cgttttgata
     2701 ggggccgaga cgcatcgaag cccatgaaca cggaacaact ctatcaggaa cgtttcaacc
     2761 gttacgtgac ggcgctgcgc aacgagaagc cggatcgggt gcccatccgg cctttcgtcg
     2821 ccgaattcac tgccaagtac gccggctaca cgtgtcagga tgtggcccat gattacccca
     2881 aggcattcga ggccgcgatc aaatgcgcga aggacttcga ctgggacgcc gtcgtaccca
     2941 acatggtcta tgtctggacc ggtctggccc aggcgatggg gctgcgctac tacggcatcc
     3001 cgggcattgg catcccgcat acggtcggct tcaactacat cgagccgccg gaggataagg
     3061 ccttcatgaa ggcggacgaa tacgatgcgt tgatcgacga tccgaccggt tttctctata
     3121 atgtctggtt cccgcgtgtt tcgaccgagg cgaaccgcat cggagagcct tcgacctacc
     3181 gcaacaacct ggcattcgtg aagggcgcga tggccatgct cgcctacttc tatgcgttcg
     3241 gcccgcagac ggcccggttg cggacggagt gtggcaccgc ctcggccatt gcgggcattt
     3301 tcaaggcccc attcgacatc attgccgaca agctgcgcgg gtacgtgggg ctgacactcg
     3361 acatgcagac ccagcccaag aaggtgcttc aggcatgtga ggcgctcatg cctcacctct
     3421 gccacatcgg cctggcgacc tcggacccca acaagcaggt gccgatcgga ttctggatgc
     3481 accgcgggtg cgtgccgctt gtcaatccgg cccagtacgc ctcgcactat tggccgacgc
     3541 tcaaacctat catcgaggaa ttctggaaga acggacgcca gactctgttc tacgccgagg
     3601 gcaaatggca ccatcacttc gattcgttcc gcgaactgcc cgaacgcagc atcgtgttcc
     3661 actgcgatcg ggacgacatc ttcgaggccc atcgccgact ccatgacaaa ttctgtctga
     3721 gcggcgggat accgaacggg ttgctcagct acgggaaacc ggatgaagtg cgggccttct
     3781 gtacccgtgt gctcgaggag gtggccgcgg agggcggata catcatggat gccggcgcca
     3841 tcatgcagga cgacaccagc atcgagaacg tgcgggtgat gaccgatgtc tgccgcgaga
     3901 agggcatcta ctcggccg
//
LOCUS       DAOQRO010000323         3916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_25294_length_3916_cov_4.380471, whole genome shotgun sequence.
ACCESSION   DAOQRO010000323 DAOQRO010000000
VERSION     DAOQRO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25294_length_3916_cov_4.380471"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKK20_11160"
     CDS             <1..304
                     /locus_tag="PKK20_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNV00488.1"
                     /translation="SHPSPYMTFTFDVAPAWRTRIPEVVHEDGTARPQIVTAESNPRY
                     HELLRKFEVKTGIGVLLNTSLNRRGEPMVCSPDDALNMFFGSDLRNLAIGDFLVVK"
     gene            410..1168
                     /locus_tag="PKK20_11165"
     CDS             410..1168
                     /locus_tag="PKK20_11165"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNV00489.1"
                     /translation="MRQGLWLNGLGRFLAAAAALLYPERCRICDVERATVAEGYVCAR
                     CRSGRYGVRWIRRPFCERCGLPFEGALTTRFECANCRGLGLHFSKARAAVLATGVVRD
                     AIHRYKYHRALWHEPFLARLLTDQAVPELLCERWDWLVPIPLHPVREREREFNQAQRL
                     AKRLSAASGIPLNVRMLLRVEPTKTQTRLTRRERAANMRQAFALRAGVSVCGQRIVLI
                     DDVLTTGATASACAQVLRAGGAGDVCVWTVARGV"
     gene            1186..2133
                     /gene="accD"
                     /locus_tag="PKK20_11170"
     CDS             1186..2133
                     /gene="accD"
                     /locus_tag="PKK20_11170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNV00490.1"
                     /translation="MATSFIPKRSIGLEGKEPPPPPEPKSAAGFIKKPKLGSGRARKR
                     EIPEGLWTKCPKCSTMIYDKELDDNLKVCPRCSHHFPIGARERIHALVETCSFEEMDP
                     QLISVDILGFTGVAAYADKLESYQRSTGLKDAVVTGIGRIGPYRVGLGVMDFSFLGGS
                     MGSVVGEKLTHLIESATDKGLGLVIVSTSGGARMYEGMFSLMQMAKTAAALAYHAQSR
                     LPYISVLTHPTTAGVMASFATLGDLIIAEPGAMIGFAGPRVIKDTTQAELPPGFQTAE
                     FLQERGLIDSIVSRKEMKERLIQYLGFLLHSQRHGIAAS"
     gene            2389..2574
                     /gene="ssrS"
                     /locus_tag="PKK20_11175"
     ncRNA           2389..2574
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKK20_11175"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2607..3425)
                     /locus_tag="PKK20_11180"
     CDS             complement(2607..3425)
                     /locus_tag="PKK20_11180"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNV00491.1"
                     /translation="MKQDRLQQQRLELKYIISEHKALAIRDYVRSYLDLDEYSQDQPD
                     YSYRIHSLYLDSDRLKTYWDTINGLKNRYKLRLRYYDDQPSSPVFFEIKRRMNDAISK
                     QRGGVHRNAVEWLLAGHLPEPRHLLSENPKELVALQRFSYLMLGIRAGPIMHVTYSRE
                     AWMSTHGNSIRVTMDRDVYCSPEFRAQLGTHLSNPVKPFGEKVILELKFTDRFPNWFR
                     ELVETFDLMRGAAAKYADGISLVGEPVFYDRASRSTSSRPSMLHPATLQPGLEN"
BASE COUNT          701 a         1154 c         1266 g          795 t
ORIGIN      
        1 cagccacccg tcgccctaca tgacgttcac gttcgacgtt gcgccggcct ggcggacgcg
       61 gattccggaa gtggtgcatg aggatggcac ggcgcgcccg cagatcgtca cggcggagag
      121 caacccgcgc taccacgagc ttctgagaaa attcgaggtg aagaccggga tcggggtgct
      181 gctgaacacc tccttgaatc gccgcggcga gccgatggtc tgctctccgg acgacgccct
      241 gaacatgttc ttcgggagcg acctgcggaa cctggcgatc ggggattttc tggttgtgaa
      301 gtgagggcgg ggtcgaaagc cccggagccg gagcgccgac gtcctctctt tgacagtcgt
      361 ttgaggatgc ggtatcggtg cgggggtgaa cacagggaga atgggctcga tgcggcaagg
      421 cctttggctg aacggcctcg gacgctttct tgcagccgcg gccgcgcttc tgtatccgga
      481 gcgttgtcga atctgtgatg tcgagagggc cacggtcgcc gagggctatg tctgtgcacg
      541 gtgccgttca ggacgctatg gtgtgcgttg gattcggcgg ccgttttgcg agcggtgcgg
      601 gctcccgttt gagggggcgt taacgacgcg gttcgaatgc gccaactgtc gcggtctggg
      661 cctgcatttc tcgaaggccc gggcggctgt cctggcgacg ggggttgtgc gggatgcgat
      721 tcaccgctac aagtatcacc gtgcgctctg gcacgagcct ttcctcgccc gtttgctcac
      781 ggaccaggcg gttccggagc ttctgtgcga gcgttgggac tggctggtgc cgattccgct
      841 tcaccccgtg cgcgagcggg agcgcgagtt caaccaggcg cagcggctgg cgaaacgcct
      901 cagcgcggcg tccgggattc cgttgaacgt ccggatgctt ctccgggtcg aaccaaccaa
      961 gacgcaaacc cggttgaccc gacgcgagcg ggcggcgaac atgcggcagg ctttcgcgtt
     1021 gagggccggg gtatcggtgt gcggacagcg gattgtgctg attgatgacg tgcttacaac
     1081 cggcgccacg gcgagcgcct gcgctcaggt ccttcgggcc gggggggcgg gcgacgtatg
     1141 cgtatggacg gtggcgcgcg gcgtctgaat gttcctgatg cttctatggc gacatcattt
     1201 atccccaagc gatcgatcgg gctcgaaggc aaggaaccgc caccgccccc tgagcccaag
     1261 agcgcggccg gtttcatcaa gaaacccaaa ctcgggtccg ggcgtgcacg caagagggaa
     1321 atcccggaag ggctgtggac caagtgcccc aagtgttcca cgatgatcta cgacaaggag
     1381 ctggacgaca acctcaaggt ctgtccccgg tgctcccacc acttcccgat cggggcccgg
     1441 gaacgcatcc atgcccttgt cgagacctgt tcgttcgagg aaatggatcc ccagttgatc
     1501 agcgtcgaca tcctcgggtt caccggcgtg gcggcctatg cggacaaact cgagagttat
     1561 cagagaagca ccgggctgaa ggacgccgtg gtgacgggca tcggccggat cggcccctac
     1621 cgtgtcgggc tgggcgtgat ggatttctcg tttctgggcg gatcgatggg atcggtggtc
     1681 ggcgagaaac tgacccatct gatcgaatcc gccaccgaca aggggctcgg cctggtgatc
     1741 gtttcgacta gcggcggcgc gcggatgtac gaagggatgt tcagcctcat gcagatggcc
     1801 aagaccgcgg ccgcgctggc ctaccatgcc caaagccgcc tgccctacat cagcgtcctg
     1861 actcacccca cgacggccgg agtcatggcc agctttgcca ccctggggga tctcatcatt
     1921 gccgagccgg gcgccatgat cggcttcgcc ggtccgcggg tgatcaagga caccacgcag
     1981 gccgaactcc cgccgggatt tcagacggcg gagttccttc aggaacgcgg cttgatcgat
     2041 tcgatcgtct cgcgcaagga gatgaaggaa cggctgattc agtacctcgg ctttctgctg
     2101 cacagccagc gccacgggat cgcggcgtcg tgaaagcctc ttctcgcctg ctctccgctc
     2161 atctcgcacc ctgtttgcgc gcacggcgat cgggcgccgc acgatgacct catctctgca
     2221 tccggtgagc gtaaatggcg ccaaactcga tgcagtggtg gggcagggtg gagcactttt
     2281 tctttttttt tgcttgagat atcggcttgg ttgggcgata agaagccgtg tcaggcggtt
     2341 gtgtgatgcg accgcggatc tttggcaaac catgaattcg acactggctt taccctgcca
     2401 ctgctcgtga ttagaaacta agtccttgaa ccgtagtctg acctagacac ggaatccgga
     2461 tgccgcacgg ttgccgacag gccttgactg gaagtcgaaa accacggcta tggttccacc
     2521 tgtttcttac aagttcaaag gaactgttac acacggcatt gtggcggggt tctttttttt
     2581 acacccgccg accggtcgcg cggccctcaa ttttcgagtc cgggttgcag cgtggccggg
     2641 tgcagcatcg agggccggga ggaggtcgat cgggatgccc ggtcgtagaa gacgggttca
     2701 ccgaccagcg agataccatc ggcatacttg gccgccgcgc cgcgcatgag atcgaacgtc
     2761 tcgaccagtt cgcggaacca attcggaaag cgatcggtga acttcagttc gagaatcacc
     2821 ttttcgccga acggcttcac tggattgctc aggtgggtgc ccagttgcgc gcggaactca
     2881 ggcgaacaat agacgtcccg gtccatggtc acgcggatcg agttgccgtg cgtgctcatc
     2941 caggcctcgc gggagtacgt gacgtgcatt attgggcctg cccggatgcc cagcatcagg
     3001 tagctgaacc tctggagcgc gaccagttcc ttgggattct ccgacagcag gtggcggggt
     3061 tcgggcaaat gacctgcgag gagccactcc accgcgttgc gatgcacacc gccgcgctgt
     3121 ttggaaatgg cgtcgttcat ccgcctcttg atttcgaaga agacgggcga ggatggttga
     3181 tcgtcgtagt accgcaatcg cagcttgtag cggtttttca acccattgat cgtgtcccag
     3241 taggtcttga gcctatccga gtcgaggtag aggctgtgaa tgcggtacga gtaatccggt
     3301 tggtcctggg agtactcgtc caagtccaga taggagcgga cgtaatcccg gattgcgagg
     3361 gccttgtgct cgctgatgat gtatttcaat tccagccgtt gctgttgcag ccggtcctgt
     3421 ttcatggctg gcctccgcgg cgatcgaaac tcaccgtaat cggatcacct cgttctcggc
     3481 tccgttggtt actctcgtcg accattttac ccaccacgat ccgcggacgg agcgcaggcg
     3541 gccggccttc gcggctgtac actcgcatcc gaatttccaa cgctctgagg ggctcctcgc
     3601 cttgcgacac cgtgatgcgc catcccgttg atgatagcag gtttagtacc aaaaaaatcg
     3661 cctcggacgc cctcgaccgc accgcaggca accgcacggg cgggcgatga cactcggtgg
     3721 ttggcggcgc aacgcgtctc tgccggcgct ggtgtgaggt gtctgccggc ccagggccgc
     3781 gcataccaaa cccgcacagc agacatcttg cgcaatctga ccgccctgtg cactcacggg
     3841 gtgtgaaaca tgcgggttag agccgcttca tttcgcgagg cctgacacat aggccaaggt
     3901 ggtccggcgc actggc
//
LOCUS       DAOQRO010000324         3912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_25334_length_3912_cov_3.789992, whole genome shotgun sequence.
ACCESSION   DAOQRO010000324 DAOQRO010000000
VERSION     DAOQRO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25334_length_3912_cov_3.789992"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKK20_11185"
     CDS             <1..846
                     /locus_tag="PKK20_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNV00492.1"
                     /translation="VDGRFIRWIPFDGIGRTVRNGLNFTERGFGVRPALGVSDRGHTA
                     ASQLRPGDFDWDHLFGNLGVRWFHTGGIFAALSDTTPQLVIEAVRKAKQYGTVVSYDL
                     NYRPSLWKSIGGPQRAKEVNREIARHVDVMLGNEEDFTACLGFHVEGADDRLLRLDAG
                     AFKRMMETAVKAYPNFKAIAITLRAVRTATRNNWAAMAWMNGRFYESRQYPDLEILDR
                     VGGGDSFAAGFIYGLMATGDPQQAVEYGAAHGALAMTTPGDTSMATRAEVETLMKGDA
                     ARVQR"
     gene            1022..2239
                     /locus_tag="PKK20_11190"
     CDS             1022..2239
                     /locus_tag="PKK20_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV00493.1"
                     /translation="MNETTRFLQSVAEGHDIEVGRLVNCLILDAARAGASDIHIEPWE
                     SSVAVRIRLSGVLVELVHLPLDLIDKVAGRLKIMANLVSYQNDLPQEGHVPASEEFGG
                     VELRISVFPTVRGEKIVIRIFDPRNRSFDMATLGFEPETLRAFIRLLSRPNGLLLLTG
                     PTGCGKTTAIYSALNHLVDRHGPTISISTVEDPVEFNLAMISQAQVRPVREFTYPIAL
                     RSLMRQDPQVIMIGEIRDPETANIAVQAGLTGHLVISTIHAGSTCGVFARLINMELEP
                     FLLASAISGVLGLRLIRTNCPDCSVPYQPEPSLMKYFPAETLTSADLRHGEGCERCLD
                     TGYSSRTALTELLEVDEVLREAILEKMPTRALQQVAIQQGMQTFWQNGLRRVLRGETT
                     IEEIMRVLAADAF"
     gene            2243..3451
                     /locus_tag="PKK20_11195"
     CDS             2243..3451
                     /locus_tag="PKK20_11195"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV00494.1"
                     /translation="MISLLLGALAVLSLALTLWQWLETRCFPLHRRIARPDFAPPLTL
                     FKPLKGGDAEMEACLRSWLRQEYPGKVQILFGVSSADEPAAPIVRALLAESPTRDAEL
                     VVCTQRLGANAKVSTIAQLSNRARHPIWVVSDADVHVPPDFLSNLVAPLKDPDVGLVN
                     SFYALDPAPTLAMRWEAIGVNADFWSSVLQSLRLAPQRFALGAAFAVRQTHVEQGGGF
                     ATLANVLADDFVLGRSVAAQNVRIALCPVVATCREEPQGWAAVWRHQVRWARTIRVCS
                     PWPYAASLAGNATLWPMLWALSDPQWTVWAGAAAFLAIRMATAWDSEARLTLSTKHWR
                     SLWMVLVKDLLQAALWALAFLGDRVEWRGERFRVLPNGELLPCNAPALGAPCTASEVS
                     PLEGKRPTAL"
     gene            complement(3518..>3912)
                     /locus_tag="PKK20_11200"
     CDS             complement(3518..>3912)
                     /locus_tag="PKK20_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNV00495.1"
                     /translation="LVIFNWQHWGIIIGSLQAIENYLDGSQNARILDRINHEAVQVYY
                     DVYNTGLTKGHDVPGDIHLLKGRIAQFHFKNGPDYLDNGKLKLGPIATAIKAIGYRGW
                     IVLETSSPSKDAVADVRRNAEFARGLFK"
BASE COUNT          700 a         1325 c         1159 g          728 t
ORIGIN      
        1 gtggatggcc gtttcatccg atggattccg ttcgacggca tcggccgcac cgtgcgcaac
       61 ggactcaact tcaccgaacg cggctttggt gtgcgccccg cgctgggcgt ctccgatcgc
      121 ggccacaccg cggccagcca gctccggccc ggcgacttcg attgggacca cctgttcgga
      181 aacctgggtg tccggtggtt tcataccggc ggcatcttcg ccgcgctcag tgacacaacg
      241 ccccaactgg tcatcgaggc cgttcgaaaa gccaagcagt acggaaccgt ggtctcgtac
      301 gacctcaatt accgcccgag cctctggaag agcattggcg gcccccaacg cgccaaagag
      361 gtgaaccgcg agatcgcgcg ccacgtcgac gtcatgctgg gcaacgagga ggatttcacc
      421 gcctgcctcg gattccacgt cgaaggggcc gatgaccgct tgcttcgcct cgatgccggc
      481 gccttcaaaa ggatgatgga aacggctgtg aaggcgtatc cgaatttcaa ggccatcgcc
      541 atcaccctgc gcgccgtccg gaccgccacg cgaaacaatt gggccgcaat ggcttggatg
      601 aatggtcgat tctacgagag ccgacagtac cccgacctcg aaatcctcga ccgcgtcggc
      661 ggaggcgaca gcttcgcggc cggtttcatt tacggcctca tggccacggg cgacccgcaa
      721 caggcggttg agtatggcgc ggctcatgga gcgcttgcca tgaccacccc gggcgacacc
      781 tcgatggcaa cgcgggcaga agtcgagaca ctgatgaaag gcgatgcagc cagggtccag
      841 cggtagcctc cacagcggtc gcctccatcc gctgataggc ccggttccct cacctggcaa
      901 gcgagcaccg acagcaacca gatgcaccgg cgaaacgccc gcctcagcgt tcaggcttgc
      961 ggaaccggca gaagcgggct tcgatctcaa ccgaaccacg cgcgcgtgta acggagaggg
     1021 catgaacgag acgacccgtt ttctgcagtc tgttgccgag ggccacgaca tcgaagtggg
     1081 ccgcttggtc aattgcctca tcctcgatgc cgcgcgcgcg ggcgccagcg acatccacat
     1141 tgaaccgtgg gaaagcagcg tcgccgtccg catccggctc tcgggcgtcc tggtcgagct
     1201 ggtgcacctc ccgctggacc tgatcgacaa ggtggccgga cggctcaaga tcatggccaa
     1261 cctggtctcc tatcaaaacg atctgcccca ggaaggccat gtgccggcca gcgaggagtt
     1321 cggcggcgtc gagctgcgca tctccgtctt ccccaccgtg cgcggcgaga agatcgtcat
     1381 tcgcatcttc gatccccgga accggagttt cgacatggcg acgctcggat tcgagccgga
     1441 gaccttgcgg gccttcattc ggctgttgtc gcgacccaac gggctgctgt tgctgacggg
     1501 gccgacgggc tgcggcaaga cgaccgcaat ctactcggcc ctgaaccacc tcgtcgaccg
     1561 ccacggcccg actatcagca tcagcaccgt cgaagatccc gtcgaattca accttgcgat
     1621 gatcagccag gcccaagtgc gccctgtgcg cgagttcacc tacccgattg ccctccgatc
     1681 cctcatgcgc caggatcccc aggtgatcat gatcggcgag attcgcgatc ccgagaccgc
     1741 taatatcgcc gtgcaggcgg gactcactgg gcatctggtg atcagcacca tccatgcggg
     1801 cagcacctgc ggcgtgttcg cgcgcctgat caacatggaa ctcgaaccct tcctcctcgc
     1861 ctccgccatc agcggcgtcc tcggcttgcg gctgatccgc accaactgcc cggactgttc
     1921 ggtcccctat cagcccgagc cgtctcttat gaagtacttt cccgccgaga ccttgacgag
     1981 cgccgacctg cggcacggtg agggctgcga gcgttgcctc gacaccgggt actccagccg
     2041 cacggccctg accgagttgc tcgaagtcga cgaggtcctg cgcgaggcca ttctcgaaaa
     2101 gatgccgacg cgcgccctcc aacaagtcgc catccagcaa ggcatgcaga ccttctggca
     2161 aaacgggctg cgccgcgtcc ttcgaggcga aacaaccatc gaagagatca tgcgcgtcct
     2221 ggccgcagac gcgttctagg caatgatcag tctcctgctc ggcgcgctcg ctgtcctcag
     2281 tctcgccctg acgctctggc agtggctcga gacgcgctgc ttccccttgc atcggcggat
     2341 cgctcgcccc gacttcgccc cgccgttgac tctgttcaaa ccgctcaagg gaggcgatgc
     2401 cgagatggaa gcctgcctcc gcagttggct caggcaagag taccccggca aagtccaaat
     2461 cctcttcggg gtttcatccg cggacgagcc cgcggcgccc atcgtccgcg cattgctggc
     2521 cgagtcgccg accagggacg ccgagttggt ggtctgtacc caacggctgg gcgccaatgc
     2581 caaggtttca accatcgccc aactctcgaa tcgcgcccgc catcccatct gggtggtcag
     2641 tgacgctgac gttcatgtcc cgcccgattt cctgagcaac ctggtcgcgc ccttgaaaga
     2701 cccagatgtg ggcctggtga attcgttcta cgccctcgac cccgcgccca ccctcgccat
     2761 gcgctgggaa gcgatcggcg tgaacgccga tttttggagc agcgtgctcc agtcattaag
     2821 gcttgcgccc cagcggtttg ctctcggagc agccttcgct gtccgacaga cccacgtcga
     2881 acaaggcggc ggtttcgcca ccctcgcaaa tgtcctcgcg gacgattttg tcctgggccg
     2941 gtccgtggcc gcccagaacg tccggattgc gctctgtccg gtggttgcca catgccggga
     3001 ggagccacaa gggtgggctg cggtctggcg acatcaagtt cgctgggcgc ggacgattcg
     3061 ggtttgctcc ccatggcctt acgccgccag cctggccggc aatgcaaccc tctggccaat
     3121 gctctgggcc ctctccgacc cgcagtggac cgtctgggcc ggtgcggccg cctttctggc
     3181 catccggatg gcaacggcgt gggacagcga agcgcgattg accctatcga ccaagcactg
     3241 gcgatcgctg tggatggtcc tagtcaagga cctcctccag gccgccctct gggctctcgc
     3301 ctttctgggc gacagggtcg agtggcgagg cgagcgcttc cgcgtcctgc ccaacggtga
     3361 gctgcttccg tgcaatgccc cagccctggg cgcgccctgc acagcgtcag aggtttcgcc
     3421 gcttgagggc aagcggccca cagcattgta ggccgggtcg cctgcctgcc ggcaggcctg
     3481 acctggcact gcatcatcta tgtcgatatg acgcgggcta tttgaaaagt ccgcgggcga
     3541 actcggcatt gcgccggaca tcggcgacgg catccttcga cgggctcgag gtttcgagca
     3601 cgatccatcc gcgataccca atcgccttga ttgcagttgc gatcggaccc aacttgagct
     3661 tcccgttgtc caggtaatcc ggtccattct tgaaatggaa ctgggcgatt cgccccttca
     3721 ggagatggat gtctcccggc acatcgtgcc ccttggtcaa cccggtgttg tacacgtcgt
     3781 agtagacctg caccgcttca tgattgatcc ggtccagaat ccgcgcgttc tgcgatccat
     3841 ccaggtaatt ctcgatggcc tgtagactgc caattattat tccccagtgc tgccaattaa
     3901 aaatcaccag tg
//
LOCUS       DAOQRO010000325         3911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_25338_length_3911_cov_4.968620, whole genome shotgun sequence.
ACCESSION   DAOQRO010000325 DAOQRO010000000
VERSION     DAOQRO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25338_length_3911_cov_4.968620"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2536)
                     /locus_tag="PKK20_11205"
     CDS             complement(197..2536)
                     /locus_tag="PKK20_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNV00496.1"
                     /translation="MNAVLSLSLVVPALLLGSHAARAVELHVAMHGHDANPGTKSSPL
                     RSIQRAANLAQPGDVITVHEGVYRERVNPPRGGASDEERIVYRAAPGERVEIKGSEIV
                     KNWVRVQDDVWKVSLTNAYFGGFNPYDDPIRGDWFDPRGRPHHTGAVYLDGEWLTEAA
                     QFEDLMLPAGTAPAWMSGGDPDYLLNVAWLRPGKGSGSEGRIPATRFAAKQGTQNAAC
                     EEGGDCIGWIAHGHWVRYDAIDFGSRTEQLELRAASATDGGLIEIRLGAPDGEVLGVC
                     PIPNTGGWQAWSSFNAAIKPISGVRSVCLVFKSRKPGASMPRPINPQLWFARVSETET
                     TLWAQFKGVNPNERCVEINVRRTVFYPDKPGRNFITVRGFALRQAATPWAPPTAEQVG
                     LIGTHWSKGWVIEDNTVSHSTCSGIALGKYGDQYDNTSANTAEGYVKTIERALANGWN
                     KATIGHHVVRRNTISHCEQAGIVGSLGAAFSTVSDNTIHDIHVRRLFSGAEMAGIKFH
                     AAIDCVIQRNHIHRTCLGLWLDWMAQGTRVSGNLFHENAGQDLFVEVNHGPFLVDNNL
                     FLSAVSLLDMSEGGAYAHNLFGGKIVSRPEPSRETPYHPAHTTEVASLANIKGGDNRF
                     YNNILVGNGPASASEGISPRKDPEWRGGYGLWVYDSREFPLRTGGNVYYHGARPYAQE
                     ANPVVREKHNPQVSLAQRDGHYFLDLTVGRELRDANTAPVSTALLGRAKIPGLAYENP
                     DSSPLEVDKDYFGKARNRRNPTPGPFERPGVGTLTLQVR"
     gene            2723..>3911
                     /locus_tag="PKK20_11210"
     CDS             2723..>3911
                     /locus_tag="PKK20_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV00497.1"
                     /translation="MRVYAALVAFVIGTGFLLLTAIDLPVSGPAMPGNALFGVLTWLS
                     LAAALSAGLFLTSDCVSEEKREGTLGLLFLTSLRGYDIVAGKLLATSIRGFHALLAIV
                     PVLAITLLMGGITGLRFAKTSVALVAAFLASLAVGLFVSALSRDSQKSMIATLVLILV
                     LAGGGPAADEIIAAVRQRPSEPMLGLTSPVSLFVMAGDSGRAPFWSGLLGNAIVAGLL
                     LGLTAVLIRHTWQQRAPARPRRVQTWMQWWRFGGTERRMALRQRLLDVNPVLWLACRE
                     RWQAVSLWVLALLLVGAFAALFIDSSGGVLEIAWHFLAIPVILLLYLGTASEATRLFV
                     EARRSGLLELMLSTPLSARRILEGQWRAWLRLFGVPLGLCLATLWVGMALVQHMTWSQ
                     MAIA"
BASE COUNT          593 a         1278 c         1271 g          769 t
ORIGIN      
        1 ccagatcatc gcccgtgagc gtgcggccct ggacgcggat cggttccact gcgccaacga
       61 tttaccccag tggccgtttc gagttcagca aaaaaatgtc caaactccag agccggctgc
      121 aagccggcgc tcgctgtgcg ttctcgaggg cgcgcgtgcc cgagcgactc cgtcgtgcgt
      181 gcgcggaatg cccacgtcac cggacctgca atgtgagcgt ccccacgccg ggccgctcga
      241 aaggcccggg cgtcggattc ctccgattgc gcgctttgcc gaagtagtcc ttgtccactt
      301 cgagaggcga gctgtccggg ttttcgtatg ccagcccggg aatcttcgct cgtcccagca
      361 gcgcagtgct caccggcgca gtgttggcgt cccgcaattc gcgaccaacg gtgagatcga
      421 gaaagtagtg tccgtcccgc tgagcgaggc tgacctgcgg gttgtgcttc tcgcgcacaa
      481 cgggattggc ctcttgggcg tacggacgcg ccccgtggta gtaaacgttg ccgccggttc
      541 ggagcggaaa ctcgcggcta tcgtacaccc agagcccata gccaccccgc cactcggggt
      601 ctttgcgggg cgagatgccc tcgcttgccg aggccgggcc gttgccgacg aggatgttgt
      661 tatagaaacg attgtcgccg cccttgatat tggccaggct ggcgacctcg gtcgtgtggg
      721 ccggatgata cggtgtttcg cggctcggct cgggccggct gacgattttg cctccaaaga
      781 gattgtgcgc ataggccccc ccctccgaca tgtcgagcag actcaccgcc gagaggaaca
      841 ggttgttatc gaccaggaac gggccgtgat tcacctcgac aaacaaatcc tggcctgcgt
      901 tctcgtgaaa gaggtttccc gagacccgcg tcccctgcgc catccaatcg agccagaggc
      961 ccagacacgt ccgatgaatg tggttgcgct gaatgacgca gtcgatggcc gcgtggaact
     1021 tgatcccggc catctcggcg cccgagaata gccgccgcac atgaatgtcg tggatggtgt
     1081 tgtccgacac ggtgctgaac gccgctccca ggctgccgac gatgcccgcc tgttcgcaat
     1141 gagagatcgt gttccgccgg acgacgtggt gtccgatcgt cgccttgttc cagccgttgg
     1201 ccagggcgcg ctcgatggtc ttgacgtatc cctcggccgt gttggccgaa gtgttgtcgt
     1261 actgatcccc gtacttgccc agggctatgc ccgaacaggt cgaatggctg accgtgttgt
     1321 cctcgatcac ccagcccttg ctccaatgcg tgccgatcaa tcccacctgc tcggcggtcg
     1381 gcggcgccca cggcgtagcc gcctgccgca gcgcgaaccc gcgcacggtg atgaagttcc
     1441 gacccggctt gtccgggtag aacaccgtgc ggcggacgtt gatctcgacg caccgctcgt
     1501 tcgggttcac gcccttgaac tgcgcccaga gagtcgtctc ggtctcgctg acccgggcga
     1561 accagagctg cgggttgatc ggacggggca tcgaggcgcc cggtttgcgg ctcttgaaga
     1621 cgaggcagac cgatctgacc ccgctgatcg gcttgatggc ggcattgaag gaggaccacg
     1681 cctgccagcc gcccgtgttc gggatcggac aaacgcccag cacctcgcca tcgggcgcgc
     1741 caagtcggat ttcgatgagc ccgccgtccg ttgccgacgc ggcgcgcagt tcgagttgtt
     1801 cggtgcgcga cccgaaatcg atcgcgtcgt agcgcaccca gtgtccatgc gcaatccacc
     1861 cgatacagtc gccgccttcc tcgcacgcgg cgttctgcgt cccttgcttc gccgcaaaac
     1921 gcgtcgcggg aatgcgccct tcgctgccgg agcccttgcc cgggcgcagc caggccacat
     1981 tcaggaggta gtccggatcg cccccgctca tccacgcggg agccgtgccg gcggggagca
     2041 tgaggtcctc gaattgggcg gcctcggtca gccactcgcc gtcgagatag accgcgccgg
     2101 tgtgatgggg gcggcctcgg ggatcgaacc agtctccacg aattggatcg tcgtagggat
     2161 tgaatccgcc gaaatacgca ttagtcagac tcactttcca cacatcgtcc tgaacccgca
     2221 cccagttctt cacgatctcc gagcctttga tctcgacccg ttcgcccggc gccgcccgat
     2281 agacgatgcg ctcttcgtcc gatgcgccgc cccggggcgg attgacccgc tcgcgataca
     2341 cgccttcgtg caccgtgatc acatcgcccg gctgcgccag attggcggca cgctggatcg
     2401 accggagcgg cgaggacttc gtgccgggat tggcgtcgtg gccgtgcatg gcgacgtgaa
     2461 gttccaccgc gcgagccgcg tgcgacccca gcagcagggc gggaacaaca agcgagagag
     2521 agaggacggc attcatggtt gacaccgttc gtacacggaa gccgcccgtt tgacaattat
     2581 cgagtcgggc ttgggacttg ccgtcaccag gcccatcggg tagcttcgcg ccacgttcca
     2641 gaaaccgaca accaccctga ctccgctgcc cgtagcccac cgcgaactcc aggtggcgtc
     2701 gcggaagcgc accacgttcc gggtgcgtgt ctacgccgct ctggtcgcct ttgtcatcgg
     2761 gaccgggttt ctcctcctga cggcaatcga cctgccagtc tcggggcccg cgatgcccgg
     2821 gaatgccctg tttggagtcc tgacgtggct gagcctggcc gccgcgttgt cggcgggttt
     2881 gtttctcacc tcggattgcg tcagcgagga gaaacgcgag ggcacgctcg gtttgctgtt
     2941 tctgacgagc ctccgtgggt acgacatcgt ggcggggaaa ctgctggcca cttcgatccg
     3001 ggggttccac gcgctgctgg cgatcgtgcc ggtccttgcg atcaccctgc tcatgggagg
     3061 catcaccgga ctgcgattcg ccaagacatc cgtcgctctc gtggccgcgt ttctcgcctc
     3121 gctggcggtt ggactgttcg tgtccgcgct cagccgggat tcgcagaagt cgatgatcgc
     3181 cacactggtc ctgattctcg tgttggcggg cggcgggccc gccgccgatg aaatcatcgc
     3241 cgcggtccgg cagcgtccgt ccgagccgat gctgggtctg acaagcccgg tttccttgtt
     3301 cgtcatggcg ggggattcgg gccgggcgcc gttttggagc ggactgctcg gcaacgccat
     3361 cgtggccgga cttctcctgg gtctgaccgc ggtcctcatc cggcacacgt ggcagcagcg
     3421 cgccccggcg cgtccgcgcc gggtacagac ctggatgcag tggtggcggt ttggcgggac
     3481 tgaacggcgc atggcgctgc gccagcgttt gctcgatgtg aatccagtcc tttggctggc
     3541 ctgtcgggag cgctggcagg ccgtgtcgct ctgggtcctg gctctcctgc tggttggggc
     3601 attcgcggct ctcttcatcg attcgagcgg gggggtgctg gagattgcgt ggcatttcct
     3661 ggccatcccg gtgatcctgc tgctctacct gggaacggcc tcggaagcca cccgcctgtt
     3721 tgttgaagcg cgccgcagcg gtctcctcga gttgatgttg tccacgccgc tctcggctcg
     3781 ccggattctc gaggggcagt ggcgcgcctg gctgcgcctg tttggagtgc cgctggggct
     3841 gtgcctggca accctctggg tcgggatggc tctggttcag cacatgacct ggagccagat
     3901 ggcgatcgcc g
//
LOCUS       DAOQRO010000326         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26021_length_3839_cov_4.656977, whole genome shotgun sequence.
ACCESSION   DAOQRO010000326 DAOQRO010000000
VERSION     DAOQRO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26021_length_3839_cov_4.656977"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2511
                     /locus_tag="PKK20_11215"
     CDS             <1..2511
                     /locus_tag="PKK20_11215"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00498.1"
                     /translation="RAAMPRFPLERGPLVLESQASPEQHFEALGEHAGVWGSLAHGVE
                     GWAYPFKLFDGWTLCLAHDDGPLADLRPFASRHVATPHMAQTVYDGGDWRLTLTWFVP
                     RSQPGMMLLLDLDGRRDLDLALRFRPVLAPMHLSATHPLDWSWDSARGEAVVREPGRQ
                     LELRVSSPTATAHRTDENGIHEIRFRLSHRQAERRHTPIAVALGCPARPGAPRVIDRL
                     LSDGGGLYRESMRHSARVIERAPHVETPNAEVNDALRWSAISLDQLRVCNPYLGCGLV
                     SGYSASGQGTRPKYCWFFEEPTLTACAYHWLGMSGHIREALRFLQRFQHASGKTAHEV
                     VQSLPFWTNYFREFPYAYMHSDGPVYYLFAYGDYYRATGDLDFLRAEWPRIRRTLDWC
                     FAAMDPADGLMRIEPGDWGSSESSVAVWKDTQLQGMWVRALRGLERLASALNEPDLAA
                     RCHAAAAQAGASIQAGLWDHTLGAYIWGLDRQGQPLKSLVPHQALSFWLGEFPPDRVR
                     RALERMAGADFRTDWGVRSLVLSDSNYDPQHYQSGSVWPVWNAGVIVADYRHERGIEA
                     YRTWLAMVRARTLDGLGPMPEVLDGRCFRRLDEGVPHQMFSELALVNGFFDGLLGLDV
                     DVPSRRIELAPTLPPAWDTLRVTRIPCGPGVLSLGLKKDRHEFQLSLDLDWPGGATVV
                     LAPHLPAGAKLWSVTLDGRPHACHIQESPAATRMRITLPHCAGKHTLRLRHADGIEWM
                     PLDDPLTPGAVSRNLRVIRAGLEGQAWRMRVEGLPGRSYPVDLFTDRDAVSTGASTRI
                     DRQKHGWRVTWTAPSSATTNAAGFVEGEISAALPARAR"
     gene            complement(2480..3016)
                     /locus_tag="PKK20_11220"
     CDS             complement(2480..3016)
                     /locus_tag="PKK20_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNV00499.1"
                     /translation="MSIPYIMDNATSFAAPRTEPAGQRILVLGLGNDILCDDAIGLRV
                     VREVRRRLRPDEEIEVEETEEMGLCLLDYISGYRGLVLVDSIQRGTLPAGEIHEIRAD
                     DLRTLSYSAPHFLGIGETLALGRELGLPMPDMIRILAIEVEEPHLLSTEMTPPLQRAL
                     PDLVQRVLTTARGLAEQR"
     gene            complement(3224..>3839)
                     /locus_tag="PKK20_11225"
     CDS             complement(3224..>3839)
                     /locus_tag="PKK20_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNV00500.1"
                     /translation="SWVDWMDFVEGPDTSVYSVAPLARLTAADGLGTPKAQAALEEFF
                     KALGGKPVHHTLANHLARAIEMIFAAERMEQLVNDPEITSPDVRRIPTSVPKEGIGVV
                     EAPRGTLFHHYQTDERGLITMANLIVATQNNAARIAMSVERAAKGLIKGGKVTEGLLN
                     KVEMAFRAYDPCLGCATHSLPGHLPLSVNIYDRDRTLVRRLEQP"
BASE COUNT          638 a         1317 c         1213 g          671 t
ORIGIN      
        1 cgggccgcga tgccgcggtt tcccctcgaa cgcggacctc tggtgctcga gagccaggcg
       61 tcgcccgaac aacacttcga agcgctgggc gaacacgcgg gcgtctgggg cagcctcgca
      121 cacggggtcg aaggctgggc ttacccgttc aaactcttcg atggctggac gctctgtctt
      181 gcccacgacg atggacccct tgccgacctg cgcccgttcg cctcacgcca cgtcgccacc
      241 ccgcatatgg cgcagaccgt gtacgatggc ggcgactggc gccttaccct cacctggttc
      301 gtcccgcgct cgcagcccgg catgatgctg ctgctcgacc tcgacgggcg acgcgatctc
      361 gacttggcgc tgcgattccg tccggtgctg gcgcccatgc acctctccgc aacgcacccg
      421 ctcgattggt cctgggattc agcacgaggc gaggcggtcg tccgcgagcc cggcaggcaa
      481 ctggagctgc gtgtgtccag cccgacggcc accgcccatc gcacggacga gaacgggatt
      541 cacgaaatcc gcttccgact gtcccatcgc caggcagaac ggcgccacac accgatcgcc
      601 gtggccttgg gctgcccggc gcgccccggc gcgccacgtg tgatcgaccg gcttctctcc
      661 gatgggggcg gcctgtatcg cgagtccatg cggcattcgg cccgcgtgat cgagcgggcg
      721 cctcatgtgg agacacccaa tgccgaggtg aacgatgcgc tgcgttggag cgcgatttcg
      781 ctcgaccagc tccgcgtctg caacccctac ctcggctgcg gcctcgtgtc gggttacagt
      841 gcctccggcc aggggacgcg cccgaaatac tgctggttct tcgaggaacc gaccctgacc
      901 gcgtgcgcgt accactggct gggaatgtcc gggcacatcc gggaggcgct gcgattcctc
      961 caacgctttc aacacgccag tggcaagacg gcgcatgagg tcgtgcagag cctcccgttc
     1021 tggaccaatt acttccgcga gttcccgtat gcctacatgc acagtgacgg ccccgtctac
     1081 tatctgttcg cctacggaga ttactatcgc gcgaccggcg atctcgactt cctccgcgcc
     1141 gagtggccgc gcatccgccg cacgctggat tggtgcttcg cggccatgga cccggcggac
     1201 ggcctgatgc gcatcgagcc cggcgactgg gggtcctcgg aaagctcggt cgccgtctgg
     1261 aaagacacgc agctccaggg catgtgggtg cgggccctgc gcggcctgga acgattggcc
     1321 agcgcgttga atgaacccga cctcgccgcg cgttgccacg ccgccgccgc gcaggccgga
     1381 gcctccatcc aggccggctt gtgggatcac acgctcggcg cctacatctg gggattggac
     1441 cgccaaggcc agcccctcaa gtcgctcgtc ccccatcaag ccctcagctt ctggctgggt
     1501 gagttcccgc cagaccgcgt ccggcgcgcg ctcgaacgga tggctggcgc ggactttcga
     1561 acggactggg gagtgcgcag tctcgttttg tcggattcaa actacgatcc gcaacactat
     1621 cagtcgggtt cagtctggcc cgtctggaat gccggagtga tcgtcgccga ctaccgacac
     1681 gagcgcggca tcgaggcgta tcgcacttgg ctggccatgg tgcgggcccg gacgctggac
     1741 ggactcggcc cgatgcccga ggtcctggat gggcgctgtt tccggaggtt ggacgagggc
     1801 gtgccgcatc agatgttctc cgaactggcc ctggtcaacg gcttcttcga cggcctcctg
     1861 ggactcgatg tcgatgttcc gtcacgacgc atcgaattgg cccccacgct gcctccggcc
     1921 tgggacaccc tgcgcgtgac ccgcattccc tgcggcccgg gcgtgttgtc cctcggattg
     1981 aagaaggaca gacacgagtt ccagctcagc ctcgatctcg attggccggg aggcgcgacg
     2041 gttgtcctgg cgccccatct acccgcaggc gcaaagctct ggtccgtcac ccttgatggc
     2101 cggccacacg catgtcatat ccaggagtcg cccgccgcaa cccgaatgcg aatcaccctg
     2161 ccccactgcg ccggcaaaca cacgctccgc ctccggcacg cggatggcat cgaatggatg
     2221 ccgctggatg acccgctcac acccggagcc gtcagccgca atctgcgcgt gatccgtgcc
     2281 ggactcgaag gccaggcgtg gcgcatgcgg gtcgagggat tgcccggacg cagctatccg
     2341 gtcgatcttt tcacggaccg cgacgcggtg tcgacaggag catcgacgcg gatcgatcgg
     2401 cagaagcacg gctggcgagt aacctggacc gcaccctcga gtgccacaac aaacgcggcg
     2461 ggcttcgtcg aaggagagat cagcgctgct ctgccagccc gcgcgcggta gtgagaacgc
     2521 gttggaccag gtcgggcaat gcccgctgga gaggcggtgt catctcggtg ctcaataagt
     2581 gcggttcctc gacctcgatc gcgaggatgc ggatcatgtc gggcattggc agccccagct
     2641 cccgccccag tgccagcgtt tcgcctatcc ccaagaaatg cggcgcggag tacgacagcg
     2701 tccggagatc gtcagcccgg atttcgtgaa tctcacccgc gggaagagtc ccccgctgga
     2761 tcgagtcgac cagaaccagt ccgcgatagc cgctgatgta atccagcagg cacaatccca
     2821 tctcctcggt ttcctcgacc tcgatctcct catcggggcg gagtcggcgc cggacctcgc
     2881 gcaccacccg cagcccgatc gcatcgtcgc agagaatgtc attccccaaa ccaagaacca
     2941 gaatgcgctg tccggccggc tcggttcgcg gcgccgcaaa tgacgtggcg ttgtccatta
     3001 tgtaagggat tgacatgcaa cgctcagatc gccatggcgc cccagctcgg atcgctcaca
     3061 gactccgagc tgaacccgat ccggcctctg gcgcggaaag acgtcgggcc acagcctcga
     3121 ccggctcggc aagcagcccc ccgtcccgat ctccccgcgt gatcgaactc gggaccggcg
     3181 attgatcgag gcgcgccgac gggccgcgcc actgtcagtg gcctcagggt tgttcgagcc
     3241 ggcgcaccag cgttcgatcg cgatcgtaga tgttgaccga caggggcaga tgacccggga
     3301 gcgaatgggt ggcgcagccc aaacagggat cgtaggcgcg gaacgccatc tcgaccttat
     3361 tcaggagccc ttcggtgacc ttgccgccct tgatcaggcc cttggcggct cgctcgacgc
     3421 tcatcgcgat ccgcgccgcg ttattctgcg tggcgacaat caggttcgcc attgtgatca
     3481 aaccccgttc gtcggtctgg tagtgatgga acagcgtgcc gcgcggggct tcgaccacgc
     3541 cgatgccttc cttgggcacg ctggtcggga tgcgtcggac atccgggctg gtgatttccg
     3601 gatcgttcac gagttgttcc atcctctcgg cggcgaagat catctcgata gcgcgcgcga
     3661 ggtggttggc aagggtatga tggacgggtt tgccgcccag ggccttgaag aactcctcga
     3721 gggcggcctg ggctttgggc gtccccaggc catcagccgc ggtcaggcgc gcaagcggcg
     3781 ccaccgaata cacactggtg tcgggcccct ccacaaaatc catccagtcc acccatgac
//
LOCUS       DAOQRO010000327         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26071_length_3835_cov_3.531481, whole genome shotgun sequence.
ACCESSION   DAOQRO010000327 DAOQRO010000000
VERSION     DAOQRO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26071_length_3835_cov_3.531481"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>784)
                     /locus_tag="PKK20_11230"
     CDS             complement(<1..>784)
                     /locus_tag="PKK20_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV00501.1"
                     /translation="TSGAGIWSHDAAHSSILIRGDHLYLNTGTGVDNTHRRIRTPDAP
                     SLVVLDKRTGRLLARDHERIAPLIYHCTWSSPSMGVVNGRPLVFFAGGDAVLYAFAPL
                     DRTPPDGDTAKLERVWKFDCDPTAPKENVHRYYQNRREGPSDIFGMPVFHDQRVYIAG
                     GGDLWWGKNEAWIQCVDATLEGDVTDTARLWSYPLVRHVMATPAVANGLVFIGDCGQT
                     VHCVDARTGKAHWTHEAGGEVWASPCIADGKVYIGTRRGQFLV"
     assembly_gap    785..794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<795..1356)
                     /locus_tag="PKK20_11235"
     CDS             complement(<795..1356)
                     /locus_tag="PKK20_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV00502.1"
                     /translation="MRAVRRIALRAAFATLLISLTAGDQPQWGHAWTRNLVYNETGLP
                     DSFDPASGRNVKWVADLGTETYSTPVIAGGRVFIGTNNEQPRDPKHQGDRGVLMCFAE
                     STGELLWQLVVPKRDEDPFFDWPRSGISSPATIEGDRVYIVSNRGEVLCLDVHGLANG
                     NDGPFRDEGRHMTPASEPALEPGPMDA"
     gene            complement(1602..2294)
                     /locus_tag="PKK20_11240"
     CDS             complement(1602..2294)
                     /locus_tag="PKK20_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00503.1"
                     /translation="MQTRPLSATTLNPIPLRGRVAFTLIELLVVIAIIAILAGMLFPL
                     LARSKEQARRMHCTNNLRQIGLGIMMYRDDHGDRPPLYLVNPTNKTYGFPGGNQPYLE
                     KGYVENTNSFICASDRTRGRIPIDLGWEYFGDFTTSYAYHLGPWFQTTTDGKKWLEDQ
                     LKRWSTRYIVAACPWHRHLFSGWTGSTYDPAKSFSRKTNVKDLCLRYDGAVDNFIWPA
                     ENWDEEPYVRLR"
     gene            2480..3604
                     /locus_tag="PKK20_11245"
     CDS             2480..3604
                     /locus_tag="PKK20_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNV00504.1"
                     /translation="MRLVLSALVLCLLPWTATRAADFHVAMIGNSMLNMGIDFGAFSS
                     AINREVEVKVAGGAGSLWKCCTLWYASKGSNRPKFVIVEVRSTELTDPLFRNTYGREA
                     IGSDLDLHAIVAAVESNPPPEVAGNHDFPAAVNHCYLPAMIQAARNGGVDLVIARHKA
                     PSYGIDENSPHDAWWDAVMKYRQDLTSCCQSNGVFFLDYQFAPEIKFAEHYLGPGDDH
                     LNAASKQIWTSLLSEDVNAILAGRRGANECTSFLRVPGDWEWQHGLSARDGLDEIADT
                     DGDGSSNYAEYVADTDPVDPKSVLAFAAGALESGRLKLTWRGGTASVQHLEARTALDG
                     SPGSWDSVRVYTPPTPAVNSFIEDGQSVPRRFYRLRAERP"
BASE COUNT          688 a         1098 c         1293 g          746 t
ORIGIN      
        1 gaccaggaat tggcctcggc gcgtgccgat atagaccttg ccgtccgcga tgcaggggga
       61 ggcccagacc tcgccgccgg cctcatgggt ccagtgagcc ttgccggtcc gggcgtcgac
      121 gcagtgcacc gtctgaccgc agtcgcctat gaagacaagg ccattcgcca cagccggcgt
      181 tgccatgacg tgccggacga gcgggtagga ccaaaggcgg gcggtgtccg tcacatcgcc
      241 ttcgagggtg gcgtcgacgc actgaatcca ggcctcgttt ttgccccacc acaagtctcc
      301 gccgcctgcg atgtagacgc gttggtcgtg gaagacgggc atgccgaaga tgtcgctggg
      361 tccttcgcgt cggttctggt agtacctgtg cacgttctct ttcggggccg tgggatcgca
      421 gtcgaacttc cagaccctct cgagtttcgc cgtgtcgcca tcgggcgggg tgcggtccag
      481 cggcgcgaac gcatacagca cggcatcgcc gccggcgaag aagacgaggg gacgtccgtt
      541 gaccacgccc atcgagggtg aggaccaggt gcaatgatag atgagcgggg cgattcgctc
      601 gtgatcgcga gccagcaggc gtccggtccg cttgtcgagc accacgagac ttggggcgtc
      661 gggcgttcga atgcggcgat gggtgttgtc cacgccggtg ccggtgttca ggtagaggtg
      721 atcgccgcgg atgaggatcg agctgtgggc ggcgtcgtgg gaccagatgc ctgcgccgct
      781 ggtcnnnnnn nnnncggcat ccatcgggcc gggctcgagc gccggctcgg aggcgggtgt
      841 catgtggcgg ccttcgtcgc ggaagggtcc gtcgttgccg tttgccaggc cgtgcacgtc
      901 gaggcagaga acctcgcccc ggttgcttac aatgtacacc cgatcccctt cgatggtggc
      961 gggggacgag ataccgcttc gaggccagtc gaagaacggg tcttcatcgc gcttgggcac
     1021 gaccagttgc catagcagct caccggtgct ctcggcgaag cacatcaaca cgccccgatc
     1081 gccctggtgc ttggggtcgc gcggctgctc gttgttcgtg ccgatgaaga ctcgtccgcc
     1141 cgcgatgacc ggcgtggaat aggtctcggt gccgaggtcg gcaacccact tgacgttgcg
     1201 ccccgacgcc gggtcgaagg aatccggcaa accggtttcg ttgtagacga gattgcgggt
     1261 ccaggcatgt ccccattgcg gctggtcgcc cgcagtcagt gaaatcagca gagtcgcgaa
     1321 agcggcgcgg agcgcgatgc gccttacggc ccgcaaagtc cttcgcaggc gaacggatga
     1381 cagaaggttc ctcatggtct tcgcatttgc attggcgcgc tacattgccc cggagatacg
     1441 ggaaagcaag gcttgaggct ggaccgcatg tttcatggag ttgcgaccac gtccatgccg
     1501 agaactccca agcgtggact catccgggcc aagggacccg cggcggtcct ccagagcata
     1561 aaccagagaa cgggcaacgc gcaatgctca gcgttcaacg ctcaacggag gcgcacgtag
     1621 ggttcctcgt cccagttttc agcgggccag atgaagttgt cgacggcgcc gtcgtagcgg
     1681 aggcagaggt ctttgacatt ggttttcctg gagaagctct tggcggggtc atacgtgctg
     1741 ccggtccacc cgctgaacag gtgtcggtgc catgggcagg ccgcgacgat gtagcgcgtg
     1801 ctccagcgct tgagctgatc ttcgagccac ttctttccgt cggtcgtcgt ttggaaccag
     1861 ggtcccaggt gataggcgta actggtagtg aagtcgccga agtattccca gcccaaatcg
     1921 atcgggattc gtccccgggt gcggtccgag gcgcagatga aggagttggt gttttcgacg
     1981 tagcccttct cgaggtaggg ctgattgccg cccgggaatc cgtaggtctt gtttgtggga
     2041 ttgaccaggt agagcggagg gcgatctcca tgatcgtcgc ggtacatcat gatgcccagg
     2101 ccgatctggc gcaggttgtt cgtgcaatgc atgcgccgcg cctgctcttt cgagcgcgcc
     2161 agcaggggga acagcatgcc ggccaggatc gcgatgatcg cgatgacaac cagcagttcg
     2221 atcagggtga aggcgacgcg cccgcgcagt gggatcgggt tcaaggtcgt tgcggagagt
     2281 ggtctcgtct gcatactcgt gctggtgctc tgaatatggc gcaacgcgac gggaaacgaa
     2341 agcccctatt tgttcgtgtt gcggagatct gcggaacagc gattctgccg acatgcggtt
     2401 gttttgcctt gagtcgcccc cgtgcctctt ggtagaagaa cgttcacatc gagcttgtat
     2461 gtttcggcat ctgacccgca tgcgtttggt tttgtcggct ctcgtcctct gtctgctgcc
     2521 gtggacagcg actcgggcag ccgactttca tgtcgcgatg atcgggaact cgatgctcaa
     2581 catgggaatc gatttcggcg cattttcgag cgcgatcaac cgcgaagtcg aggtcaaagt
     2641 cgcgggcgga gcggggagcc tgtggaagtg ctgcacgctc tggtacgctt ccaagggctc
     2701 gaatcggccc aagttcgtca tcgtcgaggt gcgcagcacc gagttgacgg accccctttt
     2761 caggaacacc tatgggcgag aggccatcgg cagcgacctc gatttgcatg cgatcgtggc
     2821 tgcggttgaa agcaaccctc cgcccgaggt tgccggcaac cacgatttcc cggcggcggt
     2881 gaaccactgc tatttgccgg ccatgattca ggcggccagg aacggcggcg tcgatctcgt
     2941 cattgctcgg cacaaagcgc cgtcttacgg catcgacgag aactcccctc atgatgcgtg
     3001 gtgggatgcg gtcatgaagt acaggcagga cctgacgagt tgctgtcagt cgaacggggt
     3061 gttctttctg gattatcagt tcgcgccgga gatcaagttc gccgagcact acttggggcc
     3121 gggcgacgac cacctgaacg ccgccagcaa gcagatatgg accagtctcc tgtcggagga
     3181 tgtgaacgcg atcctggcag ggcggcgggg cgccaacgag tgcaccagct ttctgagggt
     3241 gcctggcgac tgggagtggc agcacggatt gagcgcacgc gatggattgg atgagattgc
     3301 cgacacggat ggcgatggtt cgagcaatta cgctgaatac gtggctgata cggatcctgt
     3361 tgatccgaag tccgtcttgg cgtttgcggc aggagctctg gagtcgggtc gactcaagct
     3421 gacatggcgc ggcggcaccg cctcggtgca gcatctcgag gcccgaaccg ccctggacgg
     3481 ctcacccggg tcctgggata gcgtgcgcgt ctacacacct cccacgcccg cggtcaactc
     3541 cttcatcgag gacggtcaga gcgtcccgcg gcggttttac cggctgcggg ccgagcggcc
     3601 ttagcgcggc ctgtgtgatc gagttgtgac cgcgccgaag ccgaaagctc caaaacgcgg
     3661 accgctgcag gcgagaaggg ccacgccact ctagaatcct tgaccggtga aacacattgc
     3721 agacaagtca agtttctgtg ctgcgccaaa cgggtcaagg attctgctga gcgaggcgcg
     3781 ggagagaagg gcagggagac acggggactc ttcatgggaa ggcgaggact ggatt
//
LOCUS       DAOQRO010000328         3820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26218_length_3820_cov_3.040637, whole genome shotgun sequence.
ACCESSION   DAOQRO010000328 DAOQRO010000000
VERSION     DAOQRO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26218_length_3820_cov_3.040637"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKK20_11250"
     CDS             complement(<1..304)
                     /locus_tag="PKK20_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00505.1"
                     /translation="MIGNDNRDNVPFQLDITVSEPALVYLLIDNRRQDGSSADPPVYT
                     DGIEPEWWYSAMTWVGAEGFTPVVNGLNRAGDPFFPDEVGYDENANGTVDQWASVYF"
     gene            890..2200
                     /locus_tag="PKK20_11255"
     CDS             890..2200
                     /locus_tag="PKK20_11255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNV00506.1"
                     /translation="MGSLLLLLASGSGLGAPVLAAFVQEESHRQVEVHAVASSPSDAT
                     RVASSADTLHALETIRRQYALPALAVVVVKDGRICDRAALGVRKVGDSTPVTINDLFH
                     IGSCTKAMTATLAALLIEEGKLRWDTAIAEVFPELKGSMDRQYETVTVEQLLTHRGGC
                     PNKPPPNAWKRAWKQKGTPAEQRREFIGAVLAQPPEAAPGTKMIYSNQGYSVIGAMLE
                     RVAGESWEDLITRRLFTPLSMSSAGFGPPGTPGKVDQPWGHKRKDSVERGLERDRPSR
                     AGRESRGGIGGVHEAGDWDRFEARLAVARPDQVDNPPAIGPGGGVHCTLEDLARFTAM
                     HLEGERGGGLLRPETFRRLHSPPPGFDYACGWRSVHCDWARGQALTHDGSNTAWYVVM
                     WLAPEIRLSVVVGTNMGGSDASKGCDEVAGAMIRKWLTQPRQEE"
     gene            complement(3170..>3820)
                     /locus_tag="PKK20_11260"
     CDS             complement(3170..>3820)
                     /locus_tag="PKK20_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV00507.1"
                     /translation="KTIACGEGGAVIGDDEALMDKVYTVHNHGTSRRGKTEVIGPKYR
                     MNELEAALLLGQLDGVKERFQRRNENAAYLTSRLKGCPGLVPQKLYPGTTSGSFYLYA
                     MRYRKEHFNNVDRARFLKAMAAEGVPLSPYITIGLHREPWVDHIVQSRVYRKTFSERR
                     LKEFRDAMSCPRCDQVCEEMAMIWASGPLLGTRADMDDIADAVLKVYENRDQLASV"
BASE COUNT          690 a         1099 c         1307 g          724 t
ORIGIN      
        1 tgaaatagac cgaggcccac tggtcgacgg tcccattggc attctcgtcg tagcccactt
       61 cgtcagggaa gaacgggtcg cccgcccggt tcagtccgtt gacaaccggc gtgaatccct
      121 cggcgccgac ccaagtcatc gcggagtacc accactccgg ctcaatgccg tcggtataca
      181 cggggggatc ggcactcgat ccgtcctggc gtcggttgtc aatcagcaga tagaccagcg
      241 ccggttccga cacggtgatg tcgagctgaa acgggacgtt gtcgcgattg tcattgccga
      301 tcatttttca tggtcacgct tcgtgaaggg tcagtacaga gttgttgggt atcgcgacaa
      361 ttcatgtggt ttccgcccgc tgcgtcaacc tctttccaac acctctgcct ccacggtctt
      421 ttcatttcga gcggtgcttg actcaacaac agcaaccctt cgggaattta aatgaaatga
      481 cggtggggaa aagcaaaggg gccgcgtctg aatagttgac atgtgacgtt ctgtgatcag
      541 attggagcca gttcactttt ccattgatca gacaccggca cccatgtcac ggatcgaggt
      601 cattctcagg ccggctgcct tggtggcgca ctctgggctc ttgttgtgga gctattggca
      661 gagagttcgt ggtcgcccca aaccggcgac tgcgccgccc tggactcctg tggtgttgga
      721 cacgtcgcat ctgcgcatgt ttcgtggtgt cgtgaccgct gcgatgccgc ccactgcaaa
      781 acggggactc acgcaggcag acaaagctga gtcgcgctcc agcgcggaga ggcagcaata
      841 ccgcagactg ctgccgacac gcgaaggtct cggacaaatg gcgggggtca tgggatcgct
      901 tctgctattg cttgcaagcg gatctggatt gggtgcaccg gttctggcgg cgttcgtgca
      961 ggaggagagt catcgtcaag tcgaggtcca tgcggttgca tcaagtcctt cggatgccac
     1021 gcgcgtcgca tcgtccgccg acacgctgca cgcgctggag acgattcgca ggcagtatgc
     1081 cctgccggcg ctggcggtgg tcgtggtgaa ggacgggcgg atttgcgatc gcgccgcttt
     1141 gggggtgcga aaggtgggag attccactcc ggtgacaatc aacgacctgt tccacatcgg
     1201 gtcatgcacg aaggccatga ccgccacgct cgcggcgctc ttgatcgagg aaggaaagct
     1261 gcgctgggac accgcgattg ccgaggtctt tcccgagctg aagggcagca tggacaggca
     1321 gtacgaaacc gtcaccgtcg agcagttgtt gacccatcgc ggcggatgtc ccaacaaacc
     1381 gccgccgaac gcctggaaac gggcctggaa acagaaaggg acgcccgcgg aacagcgccg
     1441 cgagttcatc ggcgccgttc tcgcacagcc gcccgaagcg gcgccgggga caaagatgat
     1501 ttactcgaac cagggttatt ccgtcatcgg cgccatgctc gagcgggtgg ccggggaatc
     1561 atgggaagat ctcattacgc gaaggctgtt cacgccgttg agcatgagtt cggccggatt
     1621 cggtccgccc gggacgccgg gcaaagtgga tcaaccttgg ggtcacaagc gcaaggattc
     1681 ggtggagcga ggccttgagc gagatcggcc gagccgcgcg ggacgcgaga gccggggagg
     1741 catcgggggt gtccacgagg ctggggactg ggatcgcttt gaggcgcgcc tcgctgtggc
     1801 caggccggat caggtcgaca acccgccagc catcgggccc ggcgggggcg tgcactgcac
     1861 gctcgaggac ctggcgcgct tcaccgccat gcatctcgag ggggagcggg gaggcggcct
     1921 gttgcgtccg gagacgttcc gtcgactgca cagcccgcct cccggattcg actatgcctg
     1981 cggttggcgg agtgttcact gcgattgggc gcggggacag gccttgacgc acgatggttc
     2041 gaacacggcg tggtacgtgg tgatgtggct tgcgcccgag atcaggttat cggttgtggt
     2101 tgggacgaac atgggcggtt cagacgcctc gaaggggtgt gacgaagtgg ccggcgcgat
     2161 gatccggaaa tggctgacgc agccgaggca agaggagtga aaggcgttgg atcgggccgc
     2221 ctgcccaggg gatgggattg gtttgtgcgg gatgtggacg acgaccagtg ttgaatcgtg
     2281 atcgaggcga tggcggtcgc gatgcgcctc aattctccgg tgaacagagt ttcgatatgg
     2341 ccgtccccgt gctggactgt gtacgtgccc gcgggaccag acgccgaaag tcgcagggca
     2401 ttgccttcgc gccacagttc cactatgtcg ccatgtcggg ctgaggcggg ctggtcatgg
     2461 gccgcgccag gttgcgggcc ggcgtcgggg ctcttcttga gggtgggcgc ggaggcgacg
     2521 gcaacaatgg gttgtccgtc gcgggtggct gagaccacgc gctgacgaac ggctgcgtcg
     2581 gttgttgttc gaaagatcac gaacagggag ccacagggct cgaggcggag gggcagggcg
     2641 atctgtccga gcctcgatgt gacatcgcgc acgtccaccc tgcgcgtgtc ttcctcgccc
     2701 ggctcgggag agagttcggt cagcagagga gtggtatcgg gggccgagaa atgcagagtt
     2761 ctgtttgagc caccaccaat aggcgcgcac tcgggaggat cggggagggt taacccagcc
     2821 ggactcgagg ctcgacggcc tcgcttgagg cggcgcggcg ggcttgacct cgatgctggc
     2881 cggatcgcct gcgagcacat cggccgccgg cggcggatca agacaaacgg cggcaaaaaa
     2941 ggcgtatgcg gcggcccatg ccacggaggc agcagcgcga gtccaactgc gcgcggacac
     3001 tgggcgcggg caatgcttga atccgagtct gctcgtccgg tttggctggc tggcaaggcg
     3061 ttgtgaagtc atagccggtg tgtccaagca acggccattc gcaggccgat cgctcttccg
     3121 agagcggctt gggcctcagc cggacgcatc atccccgcgc gtccagcggt cagacggacg
     3181 cgagttgatc gcggttctcg taaaccttca ggacggcgtc ggcgatgtcg tccatgtccg
     3241 ccctggtgcc caggagcggt cccgaggccc agatcatcgc catttcctcg cagacctgat
     3301 cgcacctggg acagctcatc gcgtcgcgaa actccttgag ccggcgttcg gagaacgtct
     3361 tgcgatagac cctgctctga acgatgtggt ccacccaagg ctcgcggtgg agaccgatgg
     3421 tgatgtaggg gctcaagggc acgccttcgg cggccatggc tttgaggaaa cgcgcgcggt
     3481 cgacgttgtt gaagtgctcc ttgcgatagc gcatggcgta caggtagaag gagccactcg
     3541 tggtgcccgg gtagagcttc tgcggaacga ggccggggca gcccttgagg cgactcgtaa
     3601 gatacgccgc gttctcgttg cgacgttgga agcgttcctt gacaccatcg agttggccga
     3661 gcaacagcgc cgcttcgagt tcgttcatcc gatacttcgg gccgatcacc tcggtcttcc
     3721 cgcgccgcga tgtgccgtga ttgtggaccg tgtaaacctt gtccatcagg gcctcatcgt
     3781 cgccgatgac cgccccgcct tcaccgcatg caatggtctt
//
LOCUS       DAOQRO010000329         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26254_length_3816_cov_4.103962, whole genome shotgun sequence.
ACCESSION   DAOQRO010000329 DAOQRO010000000
VERSION     DAOQRO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26254_length_3816_cov_4.103962"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKK20_11265"
     CDS             complement(<1..767)
                     /locus_tag="PKK20_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV00508.1"
                     /translation="MAARDDYLLDSLVEMGYVTNEQLVPIRAEADSTGEGVVDTLVTK
                     GMLSPAVVAQAKATHFGAEFIHLSDLRLADDVIAAVPRHVAKRYSVVPVRKDESGIVV
                     AMVDPSDLDTLDGLQHALNSPIMPAVASPEDIETALSKYYGGAKDDSVSRMIQDITEG
                     EVEVGVVGGGGAAGMDDGSTVDADAPIIKLVNQMIVDAFKMRASDIHLEPLAKRFRMR
                     YRIDGVMHEMKSPPKRLQPSIISRLKIQSNMSIAERR"
     gene            1037..3514
                     /locus_tag="PKK20_11270"
     CDS             1037..3514
                     /locus_tag="PKK20_11270"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNV00509.1"
                     /translation="MNRLCTRALGLLILATMPQTFGSTTVVMCPDTAKSNAHYAGNRA
                     PLLPSPFIKLPLTAIRPEGWLRKQLELQAVGFHGHLEEVSRFMNKTNNAWLHPQGRGD
                     HGWEEVPYWLKGFGDCAYLIEDESQIREAKIWIEGAIASQQPDGWFGPGEERTGSATD
                     LKGREDMWPNMIMLMCLQSYYEYSGDPRVLDTMRRYFEYQMKVPEERFLVGYWAASRA
                     ADNLWSVHWLFNRTGEPWLLELAEKIQRRTANWTGTIPNWHNVNLAQGFGGPTFFWPQ
                     SKEAKHLAASERNWQTIRDQYGHVPGGMFGGDENCRPGYEDPRQAVETCGMVEMMFSH
                     ERLFLVTGNPIWADRCEDVAFNSLPAGLLADFKGLRYLTAPNLILSDRHNKAPGYQNG
                     GAMLLFDPYSHRCCQHNFGHGWPYFAEHLWTATPGNGLAAALYAPSRVRAKVGDGTEI
                     VIAETTHYPFEERIEFTLTAPRAVTFPLYLRIPAWCANPEVRINGAFEPIEARPLSFI
                     RLERQWNNGDAVRLDLPMEVALRTWTRNHGSVSVDRGPLTYSLKIGERYVRQGGTEKW
                     PAWEIHPTTPWNYGLVLDPIRPASSFRLVKTAWPDSEMPFTHAGVPIALTAKGRRIPE
                     WQADHLGLVGLLQDSPVRSSQPEEQITLIPMGAARLRIASFPVIGDGPEARAWKSPPP
                     SRPYKATASHVNESDTVFALKDDVVPKHSNDQEIPRFTWWDHKGTTEWVQYEFDAEQQ
                     VFGVEVYWFDDTPGGGCGVPASWRVLHQAGDDWKPVSNPQAGPVAKDRFNVMTFEPVR
                     TRALRIEVQLKPGLSGGILEWRVKSEG"
BASE COUNT          715 a         1319 c         1097 g          685 t
ORIGIN      
        1 atgcggcgtt ccgcgatcga catgttggac tggatcttga gccggctgat gatcgagggc
       61 tggaggcgct tgggagggct cttcatctcg tgcatcacgc cgtcgatccg gtaacgcatc
      121 cggaaccgct tcgccagagg ctcgaggtgg atgtccgatg cccgcatctt gaaggcatcc
      181 acgatcatct gattcaccag cttgatgatc ggcgcatccg cgtccaccgt cgatccgtcg
      241 tccatcccgg ctgcgccgcc tccgcccacc accccgacct cgacctcgcc ctcggtgatg
      301 tcctggatca tccggctgac cgagtcgtcc ttggcgccgc cgtagtactt cgaaagagcc
      361 gtctcgatgt cctcggggct ggcaacggcc ggcatgatcg ggctgttgag cgcgtgctgc
      421 agtccatcga gagtgtcgag gtcggacgga tccaccatcg ccacgacgat cccgctctcg
      481 tccttgcgca cgggcaccac actgtaacgc ttggccacat gacgcggcac cgccgcaatc
      541 acgtcatccg ccagccgcaa atcgctcagg tggatgaact cggccccgaa gtgcgtcgcc
      601 ttcgcctgag ccacaacggc cggcgacagc atccccttgg taacaagggt atccaccacc
      661 ccctcgcccg tcgaatcggc ctcggctcgg atgggcacca actgttcgtt ggtcacataa
      721 cccatctcca ccaagctgtc taaaaggtaa tcgtcgcgcg cggccacaat atcgttttct
      781 ctgttcctgg acctgtctgg accctttttc agtgggacgc tcgccatctc gtatcgggca
      841 aatccaagcc attgtcaaat ccatctcgcg ctcttctcgg attcatgcct cggccccgtc
      901 cgtgattcgg ctccgtccat tggaccaagc tgtcgaggcg ctgttacgag caggcttcgt
      961 gccggattcg tcgatttcag ttacgcggaa aggctggcgc aggcgccgcc tctctgctac
     1021 aacccgcgcc aagaatatga atcgcctatg cacgcgggct ctcgggctcc tgattcttgc
     1081 cacgatgccg cagacctttg gcagcacgac ggttgtgatg tgcccagaca ccgccaaatc
     1141 caacgctcac tacgcaggca atcgggcccc gcttctcccg agtccgttca tcaagctgcc
     1201 cctgacggca atccgacccg agggatggct gcgcaaacaa ctcgaattgc aggccgtcgg
     1261 cttccacggc catctcgagg aggtcagccg cttcatgaac aaaaccaaca acgcctggct
     1321 gcatccgcaa ggacgcggcg accatggctg ggaggaggtc ccctattggc tcaagggatt
     1381 cggcgattgc gcctatctca tcgaggacga gagccagatt cgcgaggcga agatctggat
     1441 cgaaggcgcc atcgcaagtc agcagcctga cggctggttc ggtcccggcg aggagcgcac
     1501 gggctccgcc acggatctca agggccgcga ggacatgtgg cccaacatga tcatgctgat
     1561 gtgtctgcag tcctattacg aatacagcgg cgatccccgg gtgctcgata ccatgcgccg
     1621 ctatttcgag tatcaaatga aggtgcccga agagcgattc cttgttggtt actgggccgc
     1681 cagccgcgct gccgacaacc tctggagcgt ccactggctc ttcaatcgca cgggcgaacc
     1741 gtggctgctt gaactcgccg aaaaaatcca gcgacgcacc gccaattgga ccggcaccat
     1801 tcccaactgg cacaatgtca acctcgctca gggcttcggc ggaccgactt tcttctggcc
     1861 acagtccaaa gaggccaaac acctcgccgc gtcggaacgg aattggcaga ccattcgcga
     1921 tcagtacggg cacgtccccg gagggatgtt cggcggcgat gagaactgcc gccccgggta
     1981 cgaggatccg cgccaggctg tcgagacctg cggcatggtc gagatgatgt tctcgcacga
     2041 gcgacttttc cttgtcaccg gcaacccgat ttgggccgat cggtgcgagg acgtggcgtt
     2101 caattccctc cccgccgggc tgctggccga tttcaaaggg ctgcgctatc tcacggcccc
     2161 caatctcatc ctctccgatc ggcataacaa ggcgccgggg taccagaacg gcggcgccat
     2221 gctgctcttt gacccgtatt cccaccgctg ctgccagcac aacttcggcc acggctggcc
     2281 ctattttgcc gaacacctct ggacggccac cccaggcaac gggttggccg ccgcccttta
     2341 cgccccaagc cgcgtgcgcg ccaaggtcgg cgatggcacc gaaatcgtca tcgccgagac
     2401 cacccattac ccattcgaag agcggatcga gttcaccctg accgcgcccc gcgcggtgac
     2461 atttccgctc tacctgcgca tcccggcctg gtgcgcaaac cctgaggtcc ggatcaacgg
     2521 cgccttcgaa cccatcgaag cccgcccgct gagcttcatt cgcctcgaac gccagtggaa
     2581 caatggagat gccgtccggc tcgacctgcc catggaagtc gccttgcgca cctggacgcg
     2641 caaccacggc agcgtgtcgg tcgaccgcgg cccgctcacc tactcgctga agataggaga
     2701 gcgctacgtc cgccagggcg gcaccgagaa gtggcctgca tgggaaatcc accccaccac
     2761 cccgtggaac tacggtctgg tgctcgaccc gatccgcccg gccagttcgt ttcgcctggt
     2821 caagacagcc tggcccgatt ccgagatgcc cttcacccac gcgggagttc cgatcgcact
     2881 gacggccaaa ggccggcgca tccccgaatg gcaggcggac caccttggtc tggtcggcct
     2941 gctccaggac agcccggtcc gatccagtca acccgaggag cagatcacac tcatcccgat
     3001 gggcgcagcc cggctccgca tcgcctcgtt cccggtgatc ggcgacggtc cggaggcccg
     3061 cgcgtggaaa agccccccgc cgtcccgtcc ctacaaggca acggcctcgc acgtcaacga
     3121 gagcgacacc gtgttcgcac tcaaggatga tgtcgtcccg aaacactcga acgaccagga
     3181 aatcccgcgc ttcacctggt gggaccacaa aggcaccacg gagtgggtcc agtacgagtt
     3241 cgacgccgaa caacaggttt ttggcgttga agtatactgg ttcgacgaca cgcccggcgg
     3301 cggatgcggc gtgcccgcgt cctggcgcgt gctccatcag gccggcgacg actggaaacc
     3361 cgtcagcaac ccacaggccg gcccggttgc caaagaccgc ttcaacgtga tgacgttcga
     3421 accggtccgc acccgcgccc tgcgaatcga agtgcagctc aaaccgggcc tgtccggagg
     3481 catcctcgag tggcgtgtga agtcagaagg ctgatcactc gatccgcagt gcaggaccac
     3541 ctgggcctcg ttcgctcctg acgcgagtgc ggcgccgccc agggtcaacc aggccgcaac
     3601 gagcctcagt ctcgtggtca tgaacaatac tctagaacac tgccctcccg ctgccaagcg
     3661 atcctactct cagaagcctg tcgcaaaggc gcccgcgccc ttgcggcctc gcggccagtg
     3721 catctggagg ctgtcggtgg gaatcgagtt tgcatcgcat ggggcctgcc tgccgcgcct
     3781 gcctgtgccg tgcccgacac ggcagacagt ccgctg
//
LOCUS       DAOQRO010000330         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26325_length_3809_cov_4.414491, whole genome shotgun sequence.
ACCESSION   DAOQRO010000330 DAOQRO010000000
VERSION     DAOQRO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26325_length_3809_cov_4.414491"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1115
                     /locus_tag="PKK20_11275"
     CDS             93..1115
                     /locus_tag="PKK20_11275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV00510.1"
                     /translation="MTDQSAQPSSSRRVEPGPGVRGERRSRPGEPMPGGLCSDAIERV
                     CPVCDGTQSEPFWEKGALRAVRCAQCGMVYANPVAEALATGRFYDALGTEYYLSPAKL
                     ESDYHPVRFERELRLFRSFCKRGAVLDVGCSSGAFLHHLRRCGDYRVTGVDVAGAPLD
                     YAEARGIEVVRAPFLEHDFGGRRFEAVTFWAVLEHLVNPRAFLDRAANLLVPGGHCCA
                     LVPNLGSLAIRLCGARYRYVMPDHVNYFSPATLRAFVAREDGFDLVTLRSTHFNPVVL
                     WQDWRRMRDRVPESERAQLLLRTTRLKQSRWLGPARLLYRGVEGLLGARLLADNLTIV
                     LRRRPA"
     gene            1387..2121
                     /locus_tag="PKK20_11280"
     CDS             1387..2121
                     /locus_tag="PKK20_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV00511.1"
                     /translation="MAGHSKWAKVKHFKGAIDAKRARIFAKLSREIAIAAKLGGGEPA
                     MNPRLRMVLLKCRSANMPSDNVERAIKKGTGGGDVAAYEDLTYEVFGPHGVAVLVECS
                     TDSRNRTAAEIRRLLSEHGGTLATAGGVTRLFQRKGQIIVASDAANEEQLLEIALEAG
                     AEDLKAEAEGYEILTEPGQFEAVHRAIEAQGIKCESAEVTFVPTLTVPVSGVAEGEVT
                     RLLDALEDHDDVKEVHSNAAFSGEEG"
     gene            2142..>3809
                     /locus_tag="PKK20_11285"
     CDS             2142..>3809
                     /locus_tag="PKK20_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNV00512.1"
                     /translation="MARESLMMRLLRLLARTVVRYPRLFVCPQFALACVCVFVTVDRL
                     KFDMNRNNLVGADKQYHRNFLTYAREFHGQGDLVVVVQSGDIEKKRQFVERLGRRLEG
                     EKDLFTDVFYKGDLKMMGHKALLFVPDEQVLVDMIERLKDARPVLRSFSEVTNLNGLF
                     RQVNREFRHASGEQTQEADALIKALPALARVATQAAEAIERPGLPPSPGVTALFDPGP
                     EAEENLYITLGTNRNRLYLVTARAAKEALNEEAVHRLREIVRQVQGEVPGLSVGLTGE
                     PVLEVDEMAQSQRDSTIASIVTLLLCAVLFVYSYRETGRPLKAVLCLVVGLAYTLGFT
                     TLAIGHLNLLTVTFLPILIGLGIDFGIHLVTRYEEELRGGHPEPVAMETAMVNTGIGI
                     FTGCLTTAGAFLAMGFTDFKGIEEMGLISGAGLIICLIPMLTLLPALLLRGRQNVLDH
                     HPAGTIERRGRIERMWLDRPALVLGGALSLAAAAVSQFGKVSFDYNLLHMQAHGLPAV
                     LTEQTLIDAASKSVIFGIVVADSLPEAVELDRRLRTLPTVASIDSMVP"
BASE COUNT          616 a         1055 c         1369 g          769 t
ORIGIN      
        1 gatgcccctg gccccctttc tgggctcgat tttgccagag acggcctctt tgtgctttcg
       61 ccctctgtgg gccggtccta acgtcgcctc ttgtgactga tcaaagcgca cagccttcct
      121 cgagtcgtcg cgtcgagccc ggacccggcg tgcgcgggga gcgacggtca cgcccgggcg
      181 aaccgatgcc gggcggcctg tgcagcgacg cgatcgagcg ggtctgtccc gtgtgcgacg
      241 ggacgcaatc ggaaccgttc tgggagaaag gggccttgcg tgccgttcgg tgcgcgcaat
      301 gcggcatggt gtatgcgaat cccgtggccg aggccctcgc gaccgggcgg ttttacgatg
      361 cgctgggcac ggagtactat ttgtcgccgg ccaagctcga gagtgactat catccggtgc
      421 gattcgagcg cgagctgcgc ctctttcgat cgttctgcaa gaggggtgcg gtcctggatg
      481 tcgggtgttc gagcggcgcg ttcctgcatc atctgcgccg atgcggcgat taccgggtaa
      541 caggcgtgga tgttgccgga gcgccgttgg actacgcgga ggctcgggga atcgaggtgg
      601 tacgcgcgcc gttcttggaa cacgattttg gcgggcgccg tttcgaggct gtgacgttct
      661 gggccgtgct cgagcatctg gtcaatccgc gcgcctttct cgatcgggca gcgaatctgc
      721 tggttccggg cgggcattgt tgcgctctgg ttcccaattt gggttcgttg gcgattcgcc
      781 tgtgcggggc gcggtaccgt tacgtgatgc cggatcatgt caactatttc tcgccggcga
      841 ctctgcgcgc gtttgtcgct cgggaggatg ggtttgatct ggtgactctg cgatcaacgc
      901 acttcaatcc cgtggtcttg tggcaggatt ggcgtcggat gcgcgaccgg gtgccagaga
      961 gcgagcgcgc ccagctactg ctgcggacga cgcgtctgaa acagagccga tggctggggc
     1021 cggccaggct gctttaccgc ggggtcgagg gccttctggg ggcgaggctg ctggcggaca
     1081 acctgacgat tgtgctcagg cggcgtccgg cgtgaggcgg cgctgacgct tcgccgcagt
     1141 tctcattttg gcctgggcct ccggcgagaa ccggctgaag ccgggacact caaccacgtc
     1201 cgacgcttcg gcgcggtgtt cggtgtaccg ccttcaggcg gctcgtggcc gaaatgagaa
     1261 tcgccggatg cctcacggcc tttctggaca acgggttggt ggggttggta catttcggct
     1321 gggcactccg cactcggccg cgggcaacgg gcgtcggcgg ggatggcgac aatcactttt
     1381 ttgcttatgg ctggacacag caagtgggcg aaggtcaaac acttcaaggg tgcgatcgat
     1441 gcgaagcggg cgcgcatctt tgcaaagctc tcgcgtgaga tcgccatcgc cgccaagctg
     1501 ggcggcggcg aacccgccat gaatccgcgt ctgcggatgg tgctcctgaa gtgccggagc
     1561 gcgaacatgc cgtcggacaa cgtcgaacga gccatcaaga aagggacggg gggtggcgat
     1621 gtcgcggcgt acgaagacct gacctacgag gtgttcggtc cgcacggggt cgctgtgctg
     1681 gtcgagtgca gcacggacag ccgaaaccgc acggcggcgg aaatccggcg tttgctctcc
     1741 gaacacggcg ggaccttggc cacggctggg ggtgtcacgc gtctctttca gcgcaaggga
     1801 cagatcatcg tcgcgtccga cgcggccaac gaggagcaac tgctcgagat cgcgctcgaa
     1861 gcgggggctg aggatttgaa ggcggaggct gagggctacg agattctgac ggagccgggg
     1921 caattcgagg cggttcaccg cgcgatcgag gcgcagggga tcaagtgcga gagcgcggag
     1981 gtgacgtttg tgccgacgct gaccgtgccc gtgagcgggg tggcggaggg ggaagtgacg
     2041 cggttgctcg atgcgctcga ggaccacgac gacgtgaagg aagtacattc caatgcggcg
     2101 ttctccgggg aggaaggctg agctgctctc gtgttgtcgc tgtggcgcgt gaatcgctca
     2161 tgatgcgcct gctcaggctg ctagcccgga cggtggtccg gtatccgcgg ctgtttgtct
     2221 gcccgcagtt cgctctggcg tgcgtctgcg tgttcgtgac ggttgaccga ttgaagttcg
     2281 acatgaaccg gaacaacctg gtcggggccg acaagcagta tcaccgcaat ttcctgacct
     2341 acgcgaggga gtttcacggg cagggcgacc tggtggttgt ggtccagagc ggggacatcg
     2401 agaagaagcg gcaatttgtc gagaggctgg ggcgacggtt ggagggggag aaggaccttt
     2461 tcacggatgt gttctacaag ggcgatctga agatgatggg tcacaaggcg ctcctcttcg
     2521 ttccggacga acaggtgctc gtcgacatga tcgagcggct caaggatgcc cgccctgttc
     2581 tgcggagttt cagtgaagtg acgaatctca acgggctatt tcgccaggtc aatcgggagt
     2641 ttcgtcatgc ctcgggcgag cagactcaag aggcggatgc cctgataaag gcgctgccgg
     2701 ccctggcgcg tgtggcgacc caggccgccg aggcgatcga gcggccaggc cttccgccct
     2761 cgccgggggt gacggctctg ttcgatccgg ggcccgaggc cgaggagaat ctctatatca
     2821 ccctggggac gaatcggaat cgcctctact tggtgaccgc ccgggccgcg aaagaggcct
     2881 tgaacgaaga ggccgtgcat cgcctgcgtg agattgtgcg gcaggttcag ggtgaggtgc
     2941 cgggattgag cgtggggctg acgggggaac cggtgctcga agtggacgag atggctcagt
     3001 cgcaacgcga ttcgaccatt gccagcatcg tgacattgct cctgtgcgcg gtgttgttcg
     3061 tctactcgta tcgggagacc gggcgtccgt tgaaggcggt gctgtgcctg gtggttgggc
     3121 tggcgtacac gcttgggttt accacactgg ccatcgggca tttgaatctg ctcacggtca
     3181 cgttcctgcc gatcctgatc ggattgggga tcgattttgg aatccacctg gtgacgcggt
     3241 acgaagagga attgcggggt ggccatccgg aacctgtggc catggagacc gccatggtga
     3301 acacgggcat cggaatcttc accggatgcc tgaccaccgc gggggcgttt ctggcgatgg
     3361 gtttcaccga tttcaagggc atcgaggaga tgggactcat cagcggagcg gggctgatca
     3421 tctgcctcat cccgatgctg acactgcttc cggcgctgct gctgcgcgga cggcagaacg
     3481 ttctggatca ccacccggct gggacgatcg aacggcgggg ccgcatcgag cggatgtggc
     3541 tcgatcggcc cgcgcttgtc ctgggcggcg cgctgtcgtt ggcggcggcg gccgtgtccc
     3601 agtttgggaa ggtgtcattc gactacaatc tcctgcatat gcaggcgcat gggttgcccg
     3661 cggtgctgac ggaacagaca ttgatcgacg ccgcctcgaa gtcggtgatt ttcggcattg
     3721 tggtggcgga ttcgcttccg gaggctgtcg agctcgatcg gcgtttgcgg accttgccca
     3781 cggtcgcgag catcgattcg atggttcct
//
LOCUS       DAOQRO010000331         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26526_length_3790_cov_4.282731, whole genome shotgun sequence.
ACCESSION   DAOQRO010000331 DAOQRO010000000
VERSION     DAOQRO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26526_length_3790_cov_4.282731"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PKK20_11290"
     CDS             <1..727
                     /locus_tag="PKK20_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HNV00513.1"
                     /translation="WLEHFPEIDALRGTPQDPEWHPEGDVFTHTCHCLDALVQLPEWR
                     AADSQSRIVYSLAVLGHDFGKPQTTQRVFREGRWRVVSPGHESVGGPVVEAFLARFRT
                     PEAIRRRVEPLVTNHQAHLQTVTDRSVRRLARRLEPETIHGLSVVITADAFGRPPRPR
                     ETPPGLRALQEKAAQLELQESAPKPILKGRHLCALGVPQGKAMGSLLAAAFEAQLEGR
                     FTDLEGARQWALDEIRRATDRDP"
     gene            complement(1074..1874)
                     /locus_tag="PKK20_11295"
     CDS             complement(1074..1874)
                     /locus_tag="PKK20_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV00514.1"
                     /translation="MGQGAFTLIELLVVIAIIAILAGMLLPALGRAKAKAQGIGCMNN
                     LKQLQVAWFMYVQDNQDQLPGVTGGSFAGSNTWASGWLDFSNNNPDNTNTLYLTDVRF
                     SQIGPYVQTPAIYRCPADRSMAPFGAKLYPRVRSMSMSCWMNYILNVDIGQDKYVIFR
                     KYSDIVNPGPSMTWILIDEREDSINDALFQTDLKSRGPAARIVDYPASYHGGAAGILF
                     ADGHAEIKRWRDARTTPPVKKGTAIPLNVPSPNNPDVAWLQERSSCLK"
     gene            2374..>3790
                     /locus_tag="PKK20_11300"
     CDS             2374..>3790
                     /locus_tag="PKK20_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV00515.1"
                     /translation="MNRARWILVAMTAAWVAWPIRAAESRPISLEECISLALEHNLDL
                     QIERLNTLMRNLDLWGSYSAYDPTFTGRYNHRYNMGIGGYDTSINTVTLGTEDDTDSG
                     SLGLGGLLPTGGRYELTANGSHTVGTQPAPTALDPSARMPYRTSDANAAAATLTQPLL
                     KNFWIDAARYNITLRKKELQYSEYVLQRRIMETVTAVETAYYGLIAAVEQVKVQKQAL
                     QEADQQLRENRKRVEVGVMAPLDEKDAESQVAASRANLLAAQKDLIVRQNALKALVTD
                     QYGEWAEMDLAPTESLSAVPVTFSRSDSWAKALSLRPDLLGMKVELEKRQLTLRYDRN
                     QLYPQLDIVGSYGQSGRRSTFWDALDDMREGVGNSYSYGVVLSVPLSNRQARYNYRKS
                     KQEIEQALLQLKRQEQQIMHEVEDAIAEARSSYEQIEARRQASEFAQAAYEAEQKKLE
                     NGKSTSFQVLVLQRQLTQRRYE"
BASE COUNT          740 a         1163 c         1234 g          653 t
ORIGIN      
        1 atggctcgag cactttcccg agatcgatgc cttgcgcggc acgccccagg acccggagtg
       61 gcatcccgaa ggcgacgtct tcacgcacac gtgccattgc ctggatgccc tggtccaact
      121 gcccgaatgg cgcgccgcgg attcccagtc ccgcatcgtc tacagcctgg ctgtgctggg
      181 gcacgacttc ggaaagcccc aaacgacgca gcgcgtgttc cgcgaaggac ggtggcgtgt
      241 ggtatcgccc gggcacgaaa gcgtgggcgg gccggtcgtc gaggcgtttc tggcccgatt
      301 ccgcacgccg gaagccatcc ggcggcgtgt tgagccgctg gtgaccaatc atcaggctca
      361 tctccagaca gtgaccgacc ggtcggtgcg gcggttggcc cggcgactcg agccggagac
      421 aattcatgga ctgagcgtcg tgatcaccgc ggatgccttt ggacgtccgc ccaggccacg
      481 agaaacgccg cccgggctgc gcgctcttca ggagaaggcc gcgcaactcg agttgcagga
      541 gtccgcgccg aagccgattc tcaaaggccg tcatctctgt gcccttggcg tgccgcaggg
      601 caaggcgatg ggatcgctgc tggcggctgc gttcgaggct cagctcgagg gtcggttcac
      661 cgacctcgag ggggcgcgcc agtgggcact cgatgagatt cgtcgggcca cggatcgcga
      721 cccatagccc gcatcgccca caggctgcgc gctgggccgc atccgcagcc acgatgcaag
      781 cacttgcagc catccaattc gcagggaagg gctggcgtcc agaaggagaa cagcccttac
      841 cgaatcgaca gtggagtggg gtcgtcctcg tccccgacgg atgggatcgc ggaccggatt
      901 cgtgggcgac gggaacgttg ccccaccaca cccttggcat tgtcgagaaa actcccgccc
      961 aagcagggct gttggttcta tgcatctgag ccgacattag gctgacgcct tcgcgcagcc
     1021 gcgggatcgt ccttcggacc tttgaatgcg ggtaagcgtt cgaggggcag cgactacttc
     1081 aagcaactcg accgttcctg cagccaggcc acgtccgggt tgttcggcga cggcacattc
     1141 agggggattg ccgtgccctt cttcacgggc ggcgtggtgc gggcatcccg ccagcgcttg
     1201 atctcggcat ggccgtcggc aaacaggatg cccgccgccc cgccatggta gctggccggg
     1261 taatccacaa tccgcgccgc ggggccgcga gacttcaggt cggtctggaa gagcgcgtca
     1321 ttgatgctgt cctcgcgttc gtcgatcaga atccaggtca tcgatggacc cggattcacg
     1381 atgtcggagt acttcctgaa gatgacgtac ttgtcctggc cgatatcgac attgaggatg
     1441 taattcatcc agcaactcat cgacatgctt cggactcgag gatacaactt ggcgccgaac
     1501 ggggccatgc tgcggtccgc aggacaacgg tagatcgcgg gggtctgaac gtaggggccg
     1561 atctgagaga agcggacgtc tgtcagatag agcgtgttgg tgttgtcggg gttgttattg
     1621 ctgaaatcga gccaacccga cgcccaggta ttcgagcccg caaacgagcc tccggtgacc
     1681 ccggggagct ggtcctggtt gtcctggacg tacatgaacc acgccacctg gagctgcttg
     1741 aggttgttca tgcagccgat gccctgggcc ttagccttcg ctcgccccag cgcgggcaac
     1801 agcatgcccg ccaggatggc gatgatcgca atcaccacca acagctcgat gagagtgaat
     1861 gcgccttgtc ccaagggcct tgcgccccgg acgagaacag gccgcagccc tcgatgcagt
     1921 tggtgtgtcc cggtttcgcc atttcgacac ggagtgcagg aaggaatcgg tgctaacgaa
     1981 tcgaacgtgc gcatcacgga tcatagtgtg gccccgcctc gcgctccttt caagcctcga
     2041 aaacgcagcg ccgcgatgaa ctcggaggcc agagcgccgg accgggccct tgccggttcc
     2101 aaaccggcga tacgccagat tgagaatccg cgctacgccc aaggcgcggt cgaaacatct
     2161 gagatgcgcc ttggatagga ggccgccgcg atcggcgcgg ctgccaccgc caatttatga
     2221 agtcgaaaag gtcggagtgg ggatggttcg tcccccgcca tgtccagtga agacggcgtt
     2281 tgccggcgac gccgctccac ttcatgaatt ggccctgcgc ggctgggatc ggcgtttgac
     2341 gaaccgcgtc ggatgcctca gtctccgcct ctcatgaacc gcgcacgctg gattcttgtg
     2401 gcaatgacgg ccgcttgggt tgcctggccg attcgggcgg cggagagccg cccgatttct
     2461 ttggaggagt gcatttcgct ggcgttggag cacaacttgg acctgcagat cgagcggctc
     2521 aacacgctca tgcgaaacct cgatttgtgg gggtcctatt cggcgtacga cccgaccttc
     2581 acaggccgct acaatcatcg gtacaacatg ggcatcggcg gctacgacac ctcgatcaac
     2641 accgtaaccc taggcaccga ggatgacacg gattcgggca gcttgggcct cggcgggctg
     2701 ctcccgaccg gcggtcgata cgaactgact gccaacggca gtcatactgt cggcacccag
     2761 ccggctccga cggcgttgga cccctcggcg cggatgccct atcgcactag cgatgcgaac
     2821 gcggcggcgg cgacgctgac gcagccgttg ttgaagaact tctggatcga cgcggcgcgc
     2881 tacaacatca ccttgcgcaa gaaagaactg cagtactcgg aatacgtgct gcagcgccgg
     2941 atcatggaga cggtgacggc cgtcgagacg gcctattacg gtctgatcgc cgcagtcgag
     3001 caggtcaaag tgcagaagca agctctccag gaagcggacc aacaactgcg cgaaaaccgg
     3061 aagcgggtcg aagtgggggt gatggcgccg ttggacgaga aagacgccga gtcgcaggtc
     3121 gcggccagcc gggccaacct gttggcggcg cagaaggacc tgatcgtgag gcagaacgcc
     3181 ttgaaggcgc ttgtgaccga ccagtatggg gaatgggcgg agatggattt ggcgccgacc
     3241 gaatcgcttt cggccgtgcc ggtgacgttc agccgatccg acagttgggc caaggccctg
     3301 agtttgcgcc cggacctgct cggcatgaag gtcgaactcg agaaacgcca gctcacactg
     3361 cgctacgacc gtaaccagtt gtatccgcag ttggacatcg tgggcagcta cgggcagtcg
     3421 ggacggcgta gcacgttctg ggatgcgctc gacgacatgc gggagggcgt gggcaactcg
     3481 tacagttacg gggtggttct ctcggtgccg ctcagcaacc ggcaggcccg ctacaattac
     3541 cggaagtcga aacaggagat cgagcaggcc ctgctgcaac tcaagcggca ggagcagcag
     3601 atcatgcacg aggtcgagga cgccatcgcc gaggcccgga gcagctacga gcagatcgaa
     3661 gcccgacggc aggcgagcga attcgcccaa gcggcctatg aagccgagca gaagaagctc
     3721 gaaaatggca agagcaccag cttccaagtc ctcgtcctcc agcgccaact cacacagcgc
     3781 cggtacgagg
//
LOCUS       DAOQRO010000332         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26597_length_3782_cov_6.007513, whole genome shotgun sequence.
ACCESSION   DAOQRO010000332 DAOQRO010000000
VERSION     DAOQRO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26597_length_3782_cov_6.007513"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3632
                     /locus_tag="PKK20_11305"
     CDS             <1..3632
                     /locus_tag="PKK20_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00516.1"
                     /translation="DLSGLESITGPEYSDRLLEFNVRNNGDIRLSSLRQVSQYVRFNI
                     EVPRYEMSALEAADHTAFVLGDRTVLEVPGLTSLSEGCSIGWGFNSEIKAPQLRNFVN
                     SGLSLSPGRILTVPAFTNVYGARFEVSGGRTFGVAATEYDTPANWTWYGTMFGADGAG
                     SLLDLSSLRTMRTRYCHWSGYDYRYKVMANNNGVVDLSGLESITGPEYSDRLLELTVG
                     TKGSLLLGPVDVLQYTSLRAMDSGSRLNFAALHLHAPVRLSVGTRATCAVQGDFLFEN
                     TDANSIVIEGAYLQMDGTQPQRLEVGGKDLGPTGATTRNFGYSQLIVGETNRASVVRL
                     VDTIDNGNRGAGGDPECLYLYGMDSAGLRLLNGSRLILNGLKVYALVNGQMQSLANLI
                     PAGTNSAPFGEGFIALTGGPKITNMVPAIALLPPVGSVEVSFDIPIDETSFSAGDVTM
                     LGPGGAVPITSVTKIEGNTYRLAFVPQAASGVYTVRVGPGVDELAGNLTGMDQDGDGL
                     SGEAEQDVFVGTFTIDGDAPQIVGAFSVQNGTKIGVEFDEIVDVTAATSVGTYRVNGA
                     APQQAELKDDERSVVLTVPALLGDSFELTVTDLGDALGNRATLDTIGTILGLEHRDLG
                     TPGTDPRERGSVVTFDGNRFENVAGGSAIWNKQDAGQWLFERRVGDFDVCVQLESLKK
                     TGSWTQAGLIARESADAKSRHVYVHVQYGTGNNRYAAGCRASTGGDTAYWPETPTSVH
                     VPVPNAWMRLKREGDVFIAMHGTNGTDWIEYARLTQSLPGTLLVGLATSAVNNNAGQA
                     TTAIYRHFDDLTPGFLSVPQNQALVSGATAVFGVVARGEAPLSYQWFHDGVPIDGATN
                     PTLELTNVQIADVGDYRVRVSNALGENLSTVATLSVDGVGVGGGLEADVSPSPLGDGA
                     VTIQDWVVSGRLVAALDEPVNSSQFQRADCAPAPCGDGRLSVADWTQTGLYAAALVEP
                     IPTGCGPTEPSGLIRPVGLAQQGPDGPSRHFWLKAIPSLGGLSVEVRVMVSAQGDENA
                     AGLSVVFDATALRFAGAAPGLAAADAVLQVNPRQAAEGRVGLALAQSAGAAFAAGDIE
                     LARLQFVALGTQEETAIDFGDRPIGREVVNIQAEVLEAGYQGTTIQVLARPKLESLAM
                     VKDGGLEFEFSAEAGLEWIIEVSSDLKAWTELARRVAIGGTLRFADDAPAEEGRRFYR
                     LRRVE"
BASE COUNT          694 a         1100 c         1296 g          692 t
ORIGIN      
        1 tggacctgtc ggggctggaa agcatcaccg gccccgagta cagcgaccgg ctgctcgagt
       61 ttaacgtgcg caacaacggc gacatccgtc tgtcgtcgtt gcgccaagtg agccaatacg
      121 tgcgcttcaa catcgaggtg ccgcgctacg aaatgtcggc cctggaagcg gccgaccaca
      181 cagctttcgt gttgggagat agaacagtgc tcgaggtgcc cggcctgacc tccttgagcg
      241 agggctgctc gatcgggtgg ggtttcaaca gtgagatcaa agcgccacaa ctgcgcaact
      301 tcgtcaacag cgggctgagt ctctcgcctg ggcggatact gaccgtgccc gcctttacca
      361 atgtttacgg ggcccgcttc gaggtgagcg ggggtcggac gttcggtgtg gccgcgaccg
      421 aatacgacac acctgcgaat tggacgtggt acggaacaat gtttggagcc gatggggccg
      481 ggtcgttgtt ggatctctcc tcgttgcgga cgatgcgcac gcgctactgc cattggagcg
      541 gctacgacta ccgctacaag gtgatggcaa acaacaacgg ggtggtggac ctgtcggggc
      601 tggaaagcat caccggcccc gagtacagcg accggctgct ggaactcacg gttggcacca
      661 aaggctccct gctcctcgga cctgtcgatg ttttgcagta cacaagcctg cgcgccatgg
      721 atagcggctc gcggttgaac ttcgctgcgc ttcacctcca tgccccggtc cggctttcag
      781 tcggcacccg cgctacgtgt gctgtgcaag gcgacttcct ctttgaaaac accgacgcca
      841 acagcatcgt catcgagggc gcctacctgc agatggacgg cacgcaaccg cagcggttgg
      901 aagtgggcgg gaaagacctg gggccgactg gggcgacgac acgtaatttc gggtacagcc
      961 agttgattgt gggcgagacc aaccgggcga gtgtagtgcg gttggttgac accatcgaca
     1021 atggaaatcg cggcgcgggt ggcgacccgg aatgcctcta cctctacggc atggacagcg
     1081 ctgggctgcg cctgctcaac ggctcccggc tcattctgaa tggactgaag gtgtacgcac
     1141 tggtgaacgg gcagatgcag tcgctggcca acctgatccc ggcggggacg aactcggcgc
     1201 cgttcggtga gggtttcatc gcgctcaccg ggggcccaaa gatcacgaac atggtgccgg
     1261 cgatcgccct gttgccgccg gtgggttcgg tcgaggtcag cttcgacatc ccgatcgacg
     1321 agaccagctt ctcggcgggc gatgtcacca tgctcggacc cggcggtgcc gttcccatca
     1381 catcggtgac gaagatcgag ggcaacacct atcgccttgc ctttgtgccg caagccgcca
     1441 gcggagtgta caccgtccgt gtcgggcctg gcgtcgatga actggctggc aacctgacag
     1501 ggatggatca ggacggggac ggcctgagcg gcgaggccga gcaggacgtc ttcgtgggca
     1561 cgttcacgat tgacggagac gcgccacaga ttgtcggcgc tttctcggtt cagaatggca
     1621 cgaagatcgg cgtggagttc gacgagatcg tcgatgtcac cgcggcgacg agcgtcggca
     1681 cttatagagt caacggcgca gctccgcaac aggcggaact gaaggatgac gaacgttcgg
     1741 tggtgttgac cgtcccggca ctgctgggcg attcgttcga gctgacagtg accgacttgg
     1801 gagatgcgct gggaaaccgt gcaacactcg ataccattgg aaccattctc ggcctggaac
     1861 atcgggatct gggtacgccg ggcaccgacc cgcgcgagcg gggttccgtg gtgacgttcg
     1921 atggcaaccg gttcgagaac gttgcgggcg gttcggcaat ctggaacaaa caggacgcgg
     1981 gccagtggct attcgagcgg cgtgtcggcg atttcgatgt ttgcgtccaa ctggaaagcc
     2041 tcaagaagac gggctcgtgg acccaggcgg gcttgatcgc ccgcgagtct gccgacgcaa
     2101 aaagccgtca cgtttatgtg catgtccaat acggcactgg taacaaccgc tatgcggcgg
     2161 gttgtcgcgc cagcacgggt ggcgataccg cctattggcc ggaaaccccc actagtgtcc
     2221 atgtcccggt cccgaacgcc tggatgcgtc tcaagcggga gggcgatgtc ttcatcgcca
     2281 tgcatggcac taacggcacc gactggatcg agtatgcgcg tctgacacag agcctgccag
     2341 gcacgctgct ggtgggtctg gcgacctccg cggtcaacaa caatgctggc caagcgacaa
     2401 cggccattta ccgtcacttc gacgacttga cgcccgggtt cctgtcggtg ccgcagaatc
     2461 aggccctggt ctcaggtgct accgccgtct tcggtgtggt ggcgcggggc gaagcgccgt
     2521 tgagttacca gtggttccat gatggcgtgc cgatcgacgg agcgaccaat cccaccctcg
     2581 agttgacgaa cgttcagatc gccgatgtgg gtgactaccg cgtgcgcgtg agcaacgcct
     2641 tgggcgagaa cctcagcacc gtcgccacac tgtcggtgga cggcgtcggt gtgggcggtg
     2701 gccttgaagc ggacgtatcg ccctcgccgc tgggggacgg cgccgtcacc attcaggact
     2761 gggtagtgag tggccggttg gtagcagcct tggatgagcc ggttaattcg agccagttcc
     2821 agcgggccga ctgcgcccca gccccgtgcg gagatggccg attgagtgtg gcggactgga
     2881 cgcagacagg cctgtacgcg gcggccttgg tggaaccgat tccgactggc tgcggtccca
     2941 ctgagccctc cgggttgatc cggcctgtcg gattggcgca acaggggcca gacggaccct
     3001 cgcggcactt ctggctcaaa gcgatcccgt cgctcggtgg actgtccgtc gaagtccgcg
     3061 tgatggtgtc cgcacagggc gacgagaacg cggcaggctt gagcgtagtg ttcgatgcaa
     3121 cggcactgcg tttcgccggg gcggctccag gcctggcggc ggcagacgcc gtgctgcaag
     3181 tcaacccacg gcaggcggcg gaagggcgcg ttggcctggc gctcgcgcaa agtgccggtg
     3241 cggccttcgc tgctggtgac atcgaattgg cccgcctgca gtttgtcgca ctgggaacgc
     3301 aagaggaaac tgccatcgat tttggagacc gtccgatcgg acgcgaggtc gtgaacatcc
     3361 aagcggaggt gttggaagcc gggtatcagg gcaccaccat ccaggtactg gccaggccga
     3421 agctcgagag cctggcgatg gtgaaggatg gcgggttgga gttcgagttc tcggccgaag
     3481 cgggtttgga gtggatcatc gaggtctctt ccgacttgaa ggcttggact gagttggcca
     3541 ggcgcgtagc tattggcggg acactccggt ttgctgacga cgcaccggcc gaggaaggac
     3601 ggcggttcta cagactgcgt cgcgtggagt gaatccggca agtgacccgg tatgattgtt
     3661 gatcgcgtga gcagaaccgc gacagagggt gggagaggag ccgcacagcg gatggcctgc
     3721 gcgccctcac cccggccctc tcccgtccga cgggagaggg agggtagacc ggcgccgtct
     3781 gg
//
LOCUS       DAOQRO010000333         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26740_length_3769_cov_5.699785, whole genome shotgun sequence.
ACCESSION   DAOQRO010000333 DAOQRO010000000
VERSION     DAOQRO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26740_length_3769_cov_5.699785"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKK20_11310"
     CDS             <1..1093
                     /locus_tag="PKK20_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HNV00517.1"
                     /translation="GVVNAIVLQPRGEVIVGGRFATVQAKSRASLVRLAANSQLDTTF
                     DPGLGPNGEVYCLALQPDSHILVGGLFDQVNGVPRFRLARLDASGAVDPEFDPYVASW
                     IAAPDVYSIVAQDDQRLVIGGYFDTVNGQTRSGLARLRPDGSLDSDFGGASLLDGDLP
                     IVTGIALDGDGRLLVIGTFSTVESEPRHGIARLDSEGTLDRSFDPGAGIAGGDLPAVY
                     GAALGPGGWWVIVGEFESFNGTTRLNLAVVRADGSLDPDFAPKGWMDRGALAAAVDAD
                     GNILVGGPFTTIDGKARQGLARFRSADPAPASVTIELDQATAVVSWKGPGTLQSSDSP
                     IGPWRDEPGLDSPATVSPSQPARFFRVVR"
     gene            1145..2065
                     /locus_tag="PKK20_11315"
     CDS             1145..2065
                     /locus_tag="PKK20_11315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNV00518.1"
                     /translation="MKVLYIHAHFDDYEFTAAGTFHVWRQRLGNDFRGKVIVCTDGAA
                     GHHFRTREETARLRLQEQLESARIGGYEFELLRLPCGQVPREACLRVTPEFMAALWKA
                     IRDFEPDYLLCPPLPADPLAGVHVDHLVVAQAVREVAYMINVPHAFTPEYPADETASV
                     PCTVPVIIAVYDGYMFGANTHDLAVDVEEAFDTLCAMTWCHQSQIKEWLPWVGRHHMD
                     VPRDQAEWKATLRRRFDRKNRELEIASPHAMEVFTVTAWGQIPQLERLLEDLPGLCHQ
                     ASHIDSLRERLIRWHGKAELGPDMKDKEDA"
     gene            2062..3150
                     /locus_tag="PKK20_11320"
     CDS             2062..3150
                     /locus_tag="PKK20_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV00519.1"
                     /translation="MNTNGEVERILKDAANGRPITKAEAVTLLGLPENSFGAALLRST
                     ATAVNRRRFANSGLLLGQIGVDMAPCDGDCAFCFFAKSHTTIQASILPTDAIAARCER
                     FASGGAQGVFLMTMHRFGFDWFRDLCAELRRRIPPQLEILANVGDISVAQLDELRAVG
                     VTGAYHVCRLREGVDSRMTPAARRATIERILQAGLSWYTMCEPLGPEHTPAEVADQMW
                     LGVDLPCTQHGVMQRFAVPGSPLCHYGQVSLSRLGQVAAVVTLATLGSKTVRSIAVNV
                     SNLVGLFSGANAFFPEAGEPVEQETKDAQADATLGSDGFTTALWRQSNEITTADCRAM
                     MLAAGFSHLMGTDGSPSVPLQAWAESRA"
     gene            complement(3356..>3769)
                     /locus_tag="PKK20_11325"
     CDS             complement(3356..>3769)
                     /locus_tag="PKK20_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00520.1"
                     /translation="VGLDTVDGGFTVFGRVAVGTNLLNRFNRTSVANGIFPLNLGGPL
                     YQVPVLSPAATFGDLIYSDFSLPEWPRVRIAVDAGRLRLEWNSLSNFVHHLEFASGFP
                     LSWQTLVSTNGTGQTIEVFDDRPGAASGCYRVRIE"
BASE COUNT          648 a         1250 c         1210 g          661 t
ORIGIN      
        1 cggcgtggtc aatgccatcg tcctccaacc ccggggtgag gtgatcgtgg gcggacgatt
       61 tgccaccgtg caggccaagt ctcgagccag cctggttcgc ctcgcggcca atagccaact
      121 cgacacgaca ttcgatccgg gcttggggcc caacggcgag gtttactgcc tggcgcttca
      181 gcccgacagt cacatcctgg tcggggggct gttcgaccaa gtcaacggcg tgccccgctt
      241 tcggctggcg cgcctcgacg cctcgggcgc cgtcgatccc gaattcgatc cttatgtggc
      301 gtcctggatt gccgcgccgg atgtctattc catcgttgcc caggacgacc aacgcctcgt
      361 catcggaggc tacttcgaca ctgtaaacgg ccaaacgcga tcgggtctcg cccggctgcg
      421 gcccgatggc tccctggatt ccgacttcgg cggggcgtcc ctcctcgatg gcgatctgcc
      481 gatcgtcaca ggcatcgccc tcgatggcga tggacgcctg ctcgtcatcg gcacattcag
      541 cacggtcgaa agcgaaccgc gccacggcat tgcccgcctt gactccgagg gaacactcga
      601 tcggagcttc gatcccggcg cgggcatcgc ggggggcgac ctgccggctg tctacggcgc
      661 ggcgctcgga ccgggcgggt ggtgggtcat tgtcggcgaa ttcgagtcat tcaacggcac
      721 gacgcggctc aacctggccg tcgtccgcgc cgatggctca ctcgatcctg acttcgcacc
      781 aaagggttgg atggatcgtg gggcactcgc ggccgcggtc gacgccgacg gcaatatcct
      841 ggtgggcggc ccgttcacca cgatcgacgg caaggcgcgg caagggctcg ctcgattccg
      901 atccgcggac ccggcgcccg cgtccgtcac gatcgaattg gaccaggcca cggccgtggt
      961 gtcctggaag ggacccggca ctctccagtc gagcgattcg cccatcggcc cttggcgaga
     1021 cgagcccggg ctcgacagcc ccgccaccgt gtcccccagc cagccagctc ggtttttccg
     1081 cgttgtccgc taatgtgttt cacggagcct cgatcatctc acctcgcccc agcgcttcgc
     1141 cctcatgaaa gtcctctaca tccacgcgca cttcgacgac tacgaattca ccgccgccgg
     1201 caccttccat gtctggcggc agcggctcgg gaacgatttc cgcggcaaag tcatcgtctg
     1261 caccgacggc gctgccggac accacttccg cacgcgcgag gaaacggccc gcctcaggct
     1321 gcaggaacag ctcgagtccg cgcggattgg cggttacgag ttcgagttgc tccggctgcc
     1381 gtgcggacag gtccctcgag aggcctgttt gcgtgtgaca cccgagttca tggccgccct
     1441 ctggaaggcc atccgtgact tcgaacccga ctatctgctg tgtccgccct tgccggcgga
     1501 tcccctggcc ggcgtgcatg tggaccacct ggtggtggca caggcggtgc gcgaggtcgc
     1561 ctacatgatc aatgtgccgc acgccttcac gcccgaatac ccggccgacg agacggcgtc
     1621 cgtcccctgc acggtgccgg tgatcatcgc ggtctacgac ggttacatgt tcggcgcgaa
     1681 cacccacgac ctggccgtcg acgtcgagga ggccttcgac accctctgcg ccatgacctg
     1741 gtgccaccaa tcccagatca aggaatggct gccttgggtc ggacgccatc acatggacgt
     1801 gccccgagac caggcggaat ggaaggccac tctgcgccgc cgattcgacc gcaagaaccg
     1861 ggaactcgag atcgcttccc cgcacgcgat ggaggtcttc accgtgaccg cctggggtca
     1921 gattccacag ctcgagcgac tgctcgagga cctacccggc ctgtgccatc aggccagcca
     1981 catcgattca ctccgagaac gcctgatccg ctggcacggc aaggccgagc tcggtccaga
     2041 catgaaagac aaggaagacg catgaatacg aatggagaag tcgagcgcat tctcaaggac
     2101 gccgcgaacg ggaggccgat cacgaaggct gaggccgtga cgctcctcgg ccttccggaa
     2161 aactcgttcg gggctgcgct gctgcggtcg accgcaactg ccgtcaatcg ccgacggttt
     2221 gcgaacagcg gcttgctgct cggtcagatc ggcgtggaca tggccccgtg cgacggcgac
     2281 tgcgccttct gtttcttcgc caaatcccac acaaccatcc aggcttcgat ccttccgacc
     2341 gacgcaatcg ccgcgcgttg cgagcggttt gcaagcggcg gggcgcaggg cgtctttctc
     2401 atgacgatgc atcggtttgg cttcgactgg tttcgagatc tctgcgccga gttgcgccgt
     2461 cgcataccgc cccagctcga gattctggcc aatgtcggtg acatcagcgt ggcgcaactg
     2521 gacgaactgc gcgccgttgg ggtgacgggg gcctaccatg tgtgtcgcct tcgcgaagga
     2581 gtggactccc gcatgacgcc tgccgctcgg cgggcgacaa tcgagcgcat cctccaggcc
     2641 ggactctcat ggtacaccat gtgcgagccg ctgggtccgg agcatacgcc cgcggaagtg
     2701 gcggaccaga tgtggcttgg cgtggacctg ccatgcacac aacacggagt catgcagcgt
     2761 ttcgcggtcc ccggctcgcc cctgtgccac tacggacaag tcagcctgtc ccgcttgggt
     2821 caagtggccg ccgttgtcac attggcgaca ctcgggagca agaccgtaag gagcattgcc
     2881 gtcaacgtct cgaacctggt cggcctgttc agcggcgcca atgccttctt ccccgaggct
     2941 ggagaaccgg tcgagcagga gacgaaggac gcgcaggcgg acgctacact gggcagtgac
     3001 ggattcacaa cggctctctg gcggcagagc aacgaaatca ctacggcgga ttgccgggcc
     3061 atgatgctgg cggccggttt ttcgcacctg atgggcacgg acggaagccc gagcgtgcca
     3121 ctgcaggcgt gggctgagag tcgcgcttga agagcggccc tcagttgcgc atcgtcgtga
     3181 gttcggcggc ccaggccatg tcctgctgcc gctcggatgc ggggacgcga acggagggat
     3241 cgccgctgtc ctttctgacc ggctgtctcg tgctggcgct cagccacccc ttgatttcgg
     3301 agtggccgcc ggcaaacgag aaaccggcgg cgccattgtg atggatttgg ggaatctact
     3361 cgatgcggac gcggtagcag ccactggcgg cgccgggccg gtcgtcgaac acctcgatgg
     3421 tctgcccggt cccgttggtg ctgaccagag tctgccacga caggggaaag cccgaggcga
     3481 attcgaggtg atggacgaag ttgctcaggc tgttccactc gaggcgcagg cgtccggcgt
     3541 cgacggcgat ccggacccgc ggccattccg gcagggagaa gtcgctgtag atcagatcgc
     3601 cgaacgtcgc agcgggcgac aagaccggca cctggtagag ggggccgccg agattgagcg
     3661 ggaagatgcc attggccacc gacgtccggt taaagcgatt caacaggttc gtgccgaccg
     3721 ccacacgtcc gaacaccgtg aagccgccgt cgaccgtgtc gagtccgac
//
LOCUS       DAOQRO010000334         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26821_length_3761_cov_4.525364, whole genome shotgun sequence.
ACCESSION   DAOQRO010000334 DAOQRO010000000
VERSION     DAOQRO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26821_length_3761_cov_4.525364"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PKK20_11330"
     CDS             complement(<1..1417)
                     /locus_tag="PKK20_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00521.1"
                     /translation="MKTIRSTARNLFAGSRPGWAAISLAAAILIVNPTGTGKAAQDAA
                     LSPRAGVSRRPQDRSTRPSPIEAGPHHAAWQFQTPGEGPAGTPRSAGSYIELASGLNR
                     WDAGRKAWRPAEARFDAQRDGCFVARSTQHQVILGPDLNSEGAVDLLAPDGVRLRSTI
                     LGLALVDTGSGRNVLLAEIKSCQGAQISPDEIVYPNAFDGLVADVRLLVRLDRFEQDV
                     ILRERIPPEVVSELGLDPTRTRLLVLTEFFDPPRPARKVRHLPLSDGRLLADEELSFG
                     AMSMAAGEASAAGADAPGIPVQKSWEILDGRQFLIEAVPYLALLSVSETLPAISGRRL
                     ESLRARVRRTVGVGAPERPGPGAGTGLELPGPRQASSRPHQIDAESPVRESPQRSTAQ
                     APRPSSAGLLADGVVIDYALTLVTIYRTQLDGINSHFESGATSLFAVGKYMDVIGLDA
                     GKANLKKRVVALDTRQVDIGSH"
     gene            complement(1963..2823)
                     /gene="pssA"
                     /locus_tag="PKK20_11335"
     CDS             complement(1963..2823)
                     /gene="pssA"
                     /locus_tag="PKK20_11335"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNV00522.1"
                     /translation="MADPNDHHASNPEPAGRIQIYLLPNLMTAGNLFCGFVALTKIVE
                     ADAAVGSFNTAIRHALFFILLACIFDLLDGRVARWGGHESPFGRQFDSLADLISFGAA
                     PAFLVHRIVLKDVFLEHQEIGWFIASVYLICGALRLARFNCLANLPGASGTKEFLGFP
                     IPAAAALVASLTLFIMWWEDREFPVGRWRYALPFILIFLSIMMISDVRYPSFKSINLS
                     VQRPFAKLVVAAVFIGVLIVLRDKILPLVLPIIFTAYLVYGFIRPRISRRMRREIEDA
                     DELEEPNQPP"
     gene            complement(2940..3626)
                     /locus_tag="PKK20_11340"
     CDS             complement(2940..3626)
                     /locus_tag="PKK20_11340"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNV00523.1"
                     /translation="MKHAGKATQAALRLIYWPLFFVLALMLVSVLAKFLGSILIYVAG
                     GLAVLWVLFGLFCLYFFRDPNPAIPSAPEAILAPAHGKVDTIDEIEELEFMGGRCRRV
                     SIFMSVLDVHVQRAPVSGRIAFHKRAAGQFLNALRTDSAAFNENVLLGFLSAERDGER
                     VAVRLIAGAIARRIVVWADGGTKVARGERISLIQFGSRCDLLLPLDVRIKVQLGQSVR
                     AGETVVANRP"
BASE COUNT          733 a         1102 c         1284 g          642 t
ORIGIN      
        1 agtgggatcc gatgtcgacc tgacgcgtat cgagcgccac gacgcgcttt ttcagattcg
       61 ctttgccagc gtcgagacca atcacgtcca tatacttgcc cacggcgaac aaactggttg
      121 cgcctgactc aaaatgcgaa ttgatgccgt cgagctgtgt gcggtaaatc gtgaccagcg
      181 tcagggcgta gtcgatgacc actccatcgg ccagcaggcc ggccgacgac ggtcggggag
      241 cctgtgccgt cgatcgctgg ggagactcgc gcacggggga ttcggcgtcg atttggtgtg
      301 gacggctgct ggcctggcgg ggtccaggca actcgaggcc ggttcctgcg cccggcccgg
      361 gacgctcggg cgcgccgaca ccgacggtgc ggcgcactct tgcgcggaga gattcgaggc
      421 ggcgtccgga gatggcgggc agggtctcgg agacactcag caacgcgagg tatggcacgg
      481 cttcgatcag gaactggcgc ccgtcgagga tttcccaaga cttctgcact gggattccgg
      541 gcgcatccgc accggcggcc gacgcctcgc cggcggccat ggacatggcg ccaaatgaca
      601 attcctcgtc cgccaggaga cgtccgtcgg agagcggaag gtgccggact ttcctggccg
      661 gcctgggcgg gtcgaagaac tcggtgagca cgagcagccg ggtgcgggtt ggatcgagtc
      721 cgagttcgct gaccacctcg ggcggaatcc gctccctgag gatgacgtct tgttcgaagc
      781 gatccaaacg gacgagcaga cgcacgtcgg cgacgaggcc gtcgaacgca tttgggtaga
      841 cgatttcatc gggtgagatc tgggcgccct gacagctctt gatttcagcc agcagcacat
      901 tgcgtccgga gccggtatcc accagggcca gcccgaggat ggtcgagcgc agccggactc
      961 cgtcgggcgc cagcaggtca acggcgccct cagagttgag gtcggggccc aggatcacct
     1021 gatgttgcgt ggatcgggcc acgaagcagc cgtcgcgctg agcgtcgaat cgagcttcgg
     1081 cgggtcgcca ggccttgcga ccggcatccc atcggttcaa gcccgacgcc aactcgatgt
     1141 aagagccggc ggatcgcggt gtcccggcag gaccttcacc gggagtctgg aattgccacg
     1201 ctgcatggtg cggtccggct tcgatggggc tcgggcgggt gcttctgtct tgcggtcgtc
     1261 gcgacacccc ggcgcgcggc gaaagcgccg cgtcctgtgc tgccttgcca gtccctgtcg
     1321 gattcacgat gaggattgcg gcagcgagtg agatcgcagc ccaacccggg cgcgaaccgg
     1381 cgaagagatt gcgcgcagtg gatcggattg ttttcatagt ctcctttcga cttcgtgtgt
     1441 ctggcatcga cagccgggac gggcgctgtt cgccacgcgg ggtttcggcc gaccaaccgg
     1501 ctacagtatg catcgagccg gtttgttccc cacccccacc tcttggtgtc cctccttgga
     1561 ccgccctcct tggcaggcgg cccctgggtt gttgcggcgt ggcgagaaac ctcccagttc
     1621 ccgaccgcgc ccttggcgaa acgagcagag gcaactggcg tgccaagctt caagcgaagg
     1681 cgctgcagag agcggatcgg tcgggcgagg ggggcttcag tcttagatat aagcgtttcc
     1741 tcatcaagca cttcaagcca gcgccccggc ctcgtcaggg gtgtgaattg gcaggttgac
     1801 aggcgatcgg gggctgacgg ggaggcagag agcgagcgcc ggacggtgtc ctgatgctgg
     1861 atggactgtc cggctttgag ccgccgcacg ctcctggggt tctgggagaa aggcccggtt
     1921 ggtggaggag ggatgcggct ggcaacagcg ccggcatgcg aatcaaggcg gctggtttgg
     1981 ctcttcgagt tcatcggcat cctcgatctc gcgtcgcatg cggcgcgaga tgcggggtcg
     2041 gatgaatccg tacaccaagt aggcggtaaa aatgattggg agcaccagcg ggagaatctt
     2101 atcgcgcagg acgatgagaa ccccgatgaa gaccgcggcg acgacgagtt tggcgaaggg
     2161 ccgctggacg ctcaggttaa tggatttgaa cgaaggatac ctgacatcgc tgatcatcat
     2221 gatcgacagg aagatgagga tgaacggcag ggcatagcgc cagcgcccga ctgggaactc
     2281 gcggtcttcc caccacatga tgaacaaagt cagggacgcg accagggcgg cggcggcggg
     2341 gattgggaat ccgaggaatt ccttcgtgcc gctggcgccg ggcaggttgg ccaggcagtt
     2401 gaagcgcgcc aggcgcaagg cgccgcaaat caggtagacc gaggcaatga accatccgat
     2461 ctcctggtgc tcgaggaaca cgtctttcaa cacgatgcgg tggacgagaa aagccggggc
     2521 ggcgccgaac gagatcagat cggccagcga gtcgaactgc cggccgaaag ggctttcgtg
     2581 accgccccac cgtgccacgc gcccatcgag caggtcgaaa atgcaggcaa gcaagatgaa
     2641 gaaaagcgcg tggcggatgg ctgtgttgaa actccccacg gcggcgtccg cctcgacgat
     2701 cttggtcaag gcgacaaagc cgcagaacag gttcccggcc gtcatcagat tcggcagaag
     2761 gtaaatctgg attcggccgg caggctcggg gttgctggcg tgatggtcgt ttgggtcggc
     2821 cataggatcg gcctaagggt ctgtgcaaaa attaagacac actccctccc tcgggctttg
     2881 catctggccc ggtggcgctc ccgccagaat cggaagtagt ttttgcacag accctaagct
     2941 taaggtcggt tggcgacgac ggtctcgccc gcccggaccg attggcccaa ttggaccttg
     3001 atccggacat caagcggcaa tagcagatcg cagcgagagc cgaactgaat gaggctgatg
     3061 cgttctcccc gagccacctt ggtgccgccg tcggcccaga ccacgatccg gcgcgcgatt
     3121 gcgccggcga tgagccggac cgcgaccctc tcgccgtcgc gctcggcgga caggaagccg
     3181 aggaggacgt tttcgttgaa cgcggcggaa tcggtgcgca aagcgttcag gaactggccg
     3241 gccgcccgtt tgtggaacgc gatccggccg gaaaccggag ccctctgaac gtgcacatcg
     3301 agaaccgaca tgaaaatcga cacccggcgg catcgtcctc ccatgaactc gagttcctcg
     3361 atctcgtcga tggtgtccac tttgccgtgc gcgggcgcga ggatagcctc gggggcggac
     3421 gggatggcgg ggttgggatc gcgaaagaag tagaggcaga agagtccaaa gaggacccac
     3481 aagacggcga ggccgccggc gacatagatg aggatgctgc ccaggaactt ggccagcacg
     3541 ctgacgagca tgagggccaa cacgaaaaac agcggccagt aaatcaggcg cagggccgcc
     3601 tgtgtcgctt tgcccgcgtg tttcatgcgc ccagtctaga ccaaacggcg cgcttgaaaa
     3661 gtgtggagtt cgagcctagc ccgcattttt cacaccccta tggtgcacag ggcactgaga
     3721 cttcgcaaga ggactgctgt gcgggctaga gaagcaaaac c
//
LOCUS       DAOQRO010000335         3759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26846_length_3759_cov_3.706263, whole genome shotgun sequence.
ACCESSION   DAOQRO010000335 DAOQRO010000000
VERSION     DAOQRO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26846_length_3759_cov_3.706263"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKK20_11345"
     CDS             <1..1029
                     /locus_tag="PKK20_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00524.1"
                     /translation="VAAANTPALAKIADRMVEVLPAQGACVLSDEVEDLLLLEARWRH
                     TRGVPPHVFVYANWLEFVKYHERLARQYPGRWPAIPDLAQMPEPLNPGLLTAMLGNLA
                     LTNAVYFINPSFGTYFEVLRLVPNGPIYRALTIPLDRIMPAPLTQDDLAAAQQFWEAT
                     APSIEPLVACTDSSLANAFLKGFYGRALNHWGVTLQRHGDVAGAARWFDLAARLSPDN
                     TPARVNRAYNAELRHALEPGEKPSDSIEVSVGQWDRFLRAFGPFDHPRWSLETGASFA
                     RAGFYRQALIEFERVSALSPTNTVVPLWRDNMAVMTRLRLGDTAARRNRRSHCRRRSR
                     ARRSPSRL"
     gene            984..1481
                     /locus_tag="PKK20_11350"
     CDS             984..1481
                     /locus_tag="PKK20_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV00525.1"
                     /translation="MQEAFPREEIAFEALTQVYLATGRVTNALANVERQLALNSTNAN
                     AMLNKAAFHMQLQEFPQAITTLDALLALQPQNQAALLNRAIAQLQDNRLDKAQQDYLL
                     LLKTAPEAPVVHYGLAEIDYRQTNYTAALAHYDKYLKFAPVGTDEYKAVQQRVQELRQ
                     SIRGR"
     gene            complement(1478..1615)
                     /locus_tag="PKK20_11355"
     CDS             complement(1478..1615)
                     /locus_tag="PKK20_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00526.1"
                     /translation="MRDAIAGTRARPERAWSGFAGLALGVGRFGPARALRMQTENPNA
                     D"
     gene            1614..2825
                     /locus_tag="PKK20_11360"
     CDS             1614..2825
                     /locus_tag="PKK20_11360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV00527.1"
                     /translation="MRVIHLITRLIVGGAQENTVASVLGLRRKPGLEVTLVAGLTTGP
                     EGSLESLVASESGLLTRLPALVRPVHPWLDWRAVRELTALFRKRRPDIVHTHSGKAGI
                     LGRLAARRAGVPTIIHTIHGPSFGPFQGPLANWAFCSAERYAGRFTTHFVVVANAMAD
                     QYLAAGIGRPSQYTRILSGFDLEPYLAAKPDPAWRARLGLAPDDIVVGKVARLFKLKG
                     HDDLIAAAPDLVQTCRRFKFLLVGDGLWRPTFEDRVRRLGVADHFVFAGLVPPAEVPR
                     YIGIMDVLVHLSRREGLPRALPQALAAGKPVIAFDCDGAREVCLDRETGFLLAPGDRV
                     GLRDRLLLLARDAALRGRLGGRGQALVRDAFSVDRMVDQLHDLYTRLAGTPGFDRAGE
                     HQAASRHPARL"
     gene            2822..>3759
                     /locus_tag="PKK20_11365"
     CDS             2822..>3759
                     /locus_tag="PKK20_11365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNV00528.1"
                     /translation="MKFPADAYVLAACGGFAATAATLPLWRAWCRRSGLVDDPGHRKI
                     HREPVPLAGGLAVLTGLLVPLGGAALLLASEWLAPASLRPLEYGWGRRGGQLLVLLLG
                     AIGTVVLGWWDDRRELRPALKFLGQTAIAAMVAASGIRITLFVPNPLFGYAITILWLV
                     TLMNACNFMDNMNGLCAGIGAISAFWFALKAAAAGQFLVASIAFLACGALLGFLPYNF
                     PRATVFLGDAGSHLVGYLLGVLAVLPHFYSDAHPVRLAVLNPLLILAVPLGDLVSVVV
                     IRWRLRRPVYVGDTNHLSHRLVRRGLGLQQRGEDLE"
BASE COUNT          578 a         1289 c         1180 g          712 t
ORIGIN      
        1 gtggcggcgg cgaacacgcc cgccctggcc aagattgccg atcggatggt cgaggtgttg
       61 ccggcgcaag gggcgtgcgt gctgagcgac gaagtcgagg acttgctgtt gctcgaagcg
      121 cggtggcgcc acacgcgggg tgttccgccc catgtgttcg tctatgcgaa ctggctcgag
      181 ttcgtcaaat accacgaacg gctcgccagg caatacccgg ggcgatggcc ggccatcccc
      241 gacctggccc agatgcccga accgctcaat cccggcctgc tgaccgcgat gctcgggaat
      301 ctcgcgttaa ccaacgcggt ctatttcatc aatccgagtt tcggcacgta tttcgaggtc
      361 ttacgcctgg tcccgaacgg gccgatctac cgggcgctga cgattcctct cgatcggatc
      421 atgcctgcgc cgctgactca ggacgatctg gccgcggcac aacagttctg ggaagcaact
      481 gccccctcga tcgaaccgtt ggttgcctgc acggattcga gtctggccaa cgcctttctc
      541 aaaggcttct acggacgtgc cctcaaccac tggggtgtga ctctgcaacg ccacggagat
      601 gttgctggcg cggcccgttg gttcgatctc gccgcgcgcc tgagtccaga caacacccct
      661 gcccgggtca accgggccta caacgcggag ttgcgtcatg cgctcgagcc cggggagaag
      721 cccagcgaca gcatcgaggt ctcggtgggt cagtgggacc gcttcctgcg cgcgttcggt
      781 ccgttcgatc acccccgctg gagcctggaa acgggcgcct cgttcgcgcg ggccggattc
      841 tatcgccagg cattgatcga gttcgaacga gtcagtgcgc tgtcgccgac gaatacagtc
      901 gtcccactct ggcgggacaa catggcggtc atgacccggc tccgactggg agacactgcg
      961 gcccggagaa acaggcgctc gcattgcagg aggcgttccc gcgcgaggag atcgccttcg
     1021 aggctctgac acaggtctac ctggccaccg ggcgagtgac caatgccctg gcgaacgtcg
     1081 agcggcagct cgcactcaat tcaacgaacg cgaatgcgat gctgaacaag gccgcgttcc
     1141 atatgcaact gcaggagttc ccgcaggcga ttacgacgct cgatgcgctc ctcgccctcc
     1201 agccgcagaa tcaggccgcc ttgctcaacc gcgccatcgc ccaactccag gacaaccgcc
     1261 tcgacaaggc gcaacaggat tacctcctgc tcctcaagac agctcccgaa gcccccgtgg
     1321 tccactacgg cttggctgaa atcgactacc ggcagaccaa ctacacggca gcgctcgccc
     1381 attacgacaa gtacctgaaa ttcgcccctg tgggaaccga cgagtacaag gccgtccaac
     1441 agcgtgtcca ggaactccgg caaagcattc gagggcgcta gtccgcgttc gggttctcgg
     1501 tctgcatgcg aagcgcccgg gctggtccga agcgccccac tcccagggcg aggccggcaa
     1561 acccactcca cgctcgctcc gggcgcgccc gcgtgccggc gattgcatcg cgcatgcgcg
     1621 tcattcacct gatcacacgg ttgatcgtcg gcggagcgca ggagaacacg gtggcttcag
     1681 tgcttggact ccggcgcaaa ccgggcctcg aggtcactct ggtcgccggc ctgacgaccg
     1741 gcccggaagg ctcgctcgaa tcgctggtcg cttccgaatc cggcctgctg acacggcttc
     1801 ccgcgcttgt gcgcccggtc catccctggc tcgactggcg cgccgttcgc gaactcacgg
     1861 ccctgttccg caagcgccgt ccggacatcg tccacaccca cagcggcaag gccggcatcc
     1921 ttgggcgtct ggccgcgcgg cgggctggtg tgcccacaat catccatacc attcacggcc
     1981 cctccttcgg cccgttccag gggcctctgg ccaactgggc attctgctct gcagagcgct
     2041 atgcggggag attcaccacc cacttcgtcg tcgttgccaa cgcgatggcc gaccagtacc
     2101 tggccgccgg cattggccgg ccttcccagt acacccgcat cttgagcggg ttcgacctgg
     2161 aaccctacct cgccgccaag cccgatccgg cctggcgcgc ccgcctggga ttggccccgg
     2221 acgacatcgt ggttgggaaa gtggcgcgac tgttcaaact caaaggacac gacgatctga
     2281 ttgccgccgc cccggacctg gttcagacat gccggcgctt caagtttcta ctggtcgggg
     2341 acggcctctg gcgtccgaca ttcgaagacc gtgtgcgccg gctgggtgtg gccgatcact
     2401 ttgtgtttgc gggcctggtt cctccagccg aggtcccgcg ttacatcggg atcatggatg
     2461 tgctcgtgca cctgtcgaga cgcgaaggtc tgccgcgcgc gttgccccag gccctcgccg
     2521 ccggcaaacc tgtgatcgcg ttcgactgcg acggggcccg cgaagtctgc ctcgaccgtg
     2581 aaacgggctt cctgctcgcc cccggcgatc gcgtcggtct gcgggatcgg ttgcttctcc
     2641 tggcccgcga tgccgccttg cgcggccgtc tgggaggtcg gggccaggca ttggtgcgag
     2701 acgccttttc cgtcgatcgc atggtcgatc aactccacga cctctacacc cgcttggccg
     2761 ggaccccggg attcgatcga gccggtgaac accaggcggc aagccgccac ccagcccgac
     2821 tatgaagttc cctgccgatg cctatgtgct ggcggcctgc ggcggattcg cggccaccgc
     2881 cgcaacgctt ccgctttggc gcgcctggtg tcggcgctcc gggttggtgg acgatcccgg
     2941 gcaccgcaaa atccatcggg agcctgtccc tctggccggc ggtctggcgg tgctgaccgg
     3001 cctgctggtt ccgctcgggg gcgctgcgct cctcctcgcc tccgagtggc tggctcccgc
     3061 ttctctgcgc ccgctcgaat acggctgggg acggcgcggc gggcagctcc tggtgttgct
     3121 gctgggggcg attggcacgg tcgttctggg ttggtgggac gatcggcggg agcttcgccc
     3181 ggcgttgaag ttcctcgggc agaccgcgat cgctgcaatg gtggcagcca gcggcatccg
     3241 gatcacgttg tttgtcccaa atccgctgtt tgggtatgca atcaccatcc tgtggctggt
     3301 cacgctgatg aatgcctgca atttcatgga caacatgaat ggcctctgcg ccggcatcgg
     3361 cgccatcagc gccttctggt tcgccctcaa ggccgccgcg gccgggcagt tccttgtggc
     3421 ctcgatcgct ttcctcgcct gcggagcatt gctcggattc ctgccgtaca atttcccgcg
     3481 cgccacggtc tttctgggag acgccggcag ccacctggtc ggctatcttc tgggcgtgct
     3541 ggcagtcctg ccccacttct actccgacgc gcatcccgtc cgtttggcgg tgttgaatcc
     3601 gttgctgatt cttgccgtgc cgctcggcga cctggtgtcg gtcgtcgtca tccggtggcg
     3661 cctgcggcgt ccggtctacg tgggcgacac caaccatttg tcgcatcggc ttgtccggcg
     3721 ggggctcggg ctgcaacagc gcggcgagga ccttgagca
//
LOCUS       DAOQRO010000336         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26929_length_3750_cov_7.491746, whole genome shotgun sequence.
ACCESSION   DAOQRO010000336 DAOQRO010000000
VERSION     DAOQRO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26929_length_3750_cov_7.491746"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..881)
                     /locus_tag="PKK20_11370"
     CDS             complement(210..881)
                     /locus_tag="PKK20_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00529.1"
                     /translation="MNQAVPPRKGRGCFFYGCLTLVVVVILGLAGLYLGVRSVAKQVI
                     QRYSDSSPITLPAVEISAEEAAAAQQQLKAFQSALDAGTPAEPLVLGEKEINALIAAS
                     PDFAMLKDRVHIAIDGDQIKGQISYPLDELPLPWFARIARGRYLNGSAGLSVSMTNGA
                     LLVTIQSLEVKGEAVPEQFMAGLRQQNLAQNVYKDANSMATLRKLESIAIKDGKLEVK
                     ARSDN"
     gene            complement(952..2151)
                     /gene="dinB"
                     /locus_tag="PKK20_11375"
     CDS             complement(952..2151)
                     /gene="dinB"
                     /locus_tag="PKK20_11375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNV00530.1"
                     /translation="MNPWFHSLVHLDADAFFAAVEQAADSRLRGKPVAVGGEKRGIVA
                     SASYEARRFGIETPMPTVRARKLCPQLIVLPGDFDKYERFSRWMFSYAYDFTPDVEIC
                     SIDEGYFDLTAVRRPPLEVAATIRDAIRQSLKISVSEGIGTSKLVSQIASKLRKPAAF
                     EFVSPGSEVSFLHPLPNRWLPGVGPKTAERLDAAGLALIRQVAATPVDWLGLLVGGAA
                     PQLHRFANGIDDRPVVPASQPAKSYSQQETFAQDQTDEAYLDAVLQRMADNLFAKVRA
                     DAKSIRTLTVKVRYNDFAEDQVSESLVEPTDLETDVYTRLNRMLRRAWRRRVSIRLVS
                     LKLSNLYDGLAHPELPLDRATVQRGARRRLAGIVDALRHQYGHDILMRGHDFVLRRGE
                     PAFSPLQ"
     gene            complement(2165..2809)
                     /gene="lexA"
                     /locus_tag="PKK20_11380"
     CDS             complement(2165..2809)
                     /gene="lexA"
                     /locus_tag="PKK20_11380"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNV00531.1"
                     /translation="MKTLTHRQQDVIEFIGQTIDNAGAAPTLREIAAHFRFRSVKAAA
                     DHVAALRRKGVLAGPPRRARALRIVDAVGDWATARALGRRLARLPVYGTIPAGLAEDR
                     RQEARGCVAVDLDTLGIRPTPRTFALEVRGDSMIGRHIVDGDLVVLEHGKMPRHGDVV
                     AALIDNESTLKTFVQERGRPPCLKAENPRYPKLHPVGELVIQGVMVGLIRKVKV"
BASE COUNT          706 a         1143 c         1209 g          692 t
ORIGIN      
        1 gtgaatcccg tcgatgactg tcaccgtccc gaattgaacc acagagacac agagaccaca
       61 gaggacagcg catccaaaga gccacagatg aaattctgtg gattaaggtc ttctctgtgt
      121 tctctgcgtc tctgtggtga aatcccgtcg atgactgtca gcgtgccgaa cagttggtga
      181 gaaatgcggg ctacagcgag cgaccgtggt cagttgtcgg accgggcttt gacctcgagt
      241 ttgccgtctt tgatggcgat ggattcgagt ttgcggagcg tcgccatgct gttggcgtcc
      301 ttgtagacgt tctgcgccag gttctgctgg cgcagcccag ccatgaattg ctcgggcacg
      361 gcctcgccct tgacttcgag actctggatc gtcacgagga gggcgccatt agtcatcgat
      421 acgctcaagc cggccgagcc gttgaggtag cggccccggg cgatccgggc aaaccagggc
      481 agcggcagtt catcgagcgg gtaactgatc tggcccttga tctggtcgcc gtctatcgcg
      541 atgtggactc ggtccttgag catggcgaag tcgggcgagg cggcaatcaa ggcgttgatt
      601 tccttctcgc cgagcaccag cggttcggcg ggcgtcccgg catcgagggc ggactgaaag
      661 gctttcaact gttgctgggc ggcggcggcc tcctcggccg aaatctcgac cgccggcagt
      721 gttatcgggc tcgaatcgct gtacctctgg atcacctgtt tggcgacgga gcggacgccc
      781 aggtagaggc cggccaggcc gaggatcaca accaccacga gggtcaaaca cccgtagaag
      841 aaacaaccgc ggcctttgcg ggggggaaca gcttggttca tagaactcgg gattgtttgt
      901 gcgcaccgca ccatgccgca gtgccgggtg gaagacaacg cgaaaatcgg ctcattgaag
      961 aggcgaaaac gccggctcgc cccggcgcag gacgaagtcg tgtccgcgca tcaggatgtc
     1021 gtgtccgtac tgatggcgca acgcgtccac gatgcccgcc aagcggcggc gggcgccgcg
     1081 ttggaccgtc gcgcgatcca gcgggagttc gggatgggcg aggccgtcgt aaaggttcga
     1141 cagcttcaac gagaccaggc ggatgctgac gcgccggcgc caggcgcgtc gcaacatccg
     1201 gttgagccgc gtgtagacat cggtctcgag gtccgtcggc tcgaccaggc tttcgctgac
     1261 ctggtcttcg gcaaagtcat tgtagcgcac cttgaccgtg agtgtgcgaa tgctcttggc
     1321 gtccgcgcgg accttggcaa acaggttgtc tgccatccgt tgcaggacgg cgtccagata
     1381 cgcttcgtcg gtctgatcct gggcgaaggt ttcttgctgg ctgtaactct tggccggttg
     1441 gctggcgggg acgacggggc ggtcatcgat gccgttggcg aaccgatgca actggggcgc
     1501 cgccccgccg accaacaggc ccagccaatc gactggcgtg gcggcgacct ggcggatgag
     1561 ggcgagtccg gccgcgtcga ggcgctcggc tgtcttgggc cccacgcccg gcagccagcg
     1621 gttgggcaga ggatgcaaaa acgatacctc gcttcccggc gacacaaact cgaaggccgc
     1681 cggcttgcgg agtttcgagg cgatttggct gacgagtttg ctggtgccga tgccttcgct
     1741 gacgctgatc ttgagcgatt ggcgaatggc gtcacggatg gtggcggcga cttcgagcgg
     1801 cggacgccgc acagccgtga gatcgaaata cccctcgtcg atcgagcaga tttcgacgtc
     1861 gggcgtgaaa tcgtaggcgt agctgaacat ccagcgcgaa aaacgctcgt atttgtcgaa
     1921 gtcgcccggc agcacgatga gttgcggaca gagcttgcgc gcccgcacgg tgggcatcgg
     1981 cgtctcaatc ccgaaacgcc gcgcctcgta ggacgcgctg gccacaatgc cgcgtttctc
     2041 gccgccgacg gcgacgggtt tgccgcgcaa ccgcgaatcc gccgcctgct cgaccgccgc
     2101 gaagaaggcg tcggcatcca gatgcaccag gctatgaaac cacggattca ccccttttcg
     2161 gtggttacac cttcactttc cggatcagcc ccaccatcac cccctggatc accagttcgc
     2221 cgaccggatg gagtttgggg tagcgcggat tctcagcctt caaacacggc ggacgccccc
     2281 gttcctgcac gaatgtcttg agtgtcgatt cgttgtcgat cagtgccgcc acaacatcgc
     2341 cgtgacgcgg catcttgcca tgctcgagca ccaccagatc gccgtcgaca atgtggcgtc
     2401 caatcatcga atcgccccgc acttcgagcg cgaaggtgcg cggggtgggg cggattccca
     2461 gtgtgtccag atcgaccgcc acgcatccgc gggcttcctg ccggcgatcc tcggcaagac
     2521 cggcggggat ggttccgtag acgggcaatc gcgccaggcg ccgccccaac gcccgcgccg
     2581 tcgcccaatc gccgacggcg tccacgatcc tcagggctcg ggcgcgtcgg ggcgggccgg
     2641 ccaagacgcc tttgcgacgc aaggcggcga cgtgatcggc ggcggccttg acactgcgga
     2701 agcggaaatg cgcggcgatt tcccgcaagg tgggcgccgc gccggcgttg tcgatggtct
     2761 gtccaatgaa ctcgatcacg tcctgctgcc gatgagtcag agttttcatg gttactatac
     2821 agatgtatag tgtatagtaa ttggaggcga tggtttggca agggcagaat tgttgccgtt
     2881 ctcatggtct gcaaaccgct ggccgcctcc gaagtgattt tatccagcaa tcgagagcta
     2941 ttaagccgca tttctcacca actgtcgatg tcatctgacc ggacaccgtc acatttcacc
     3001 tgaaatgcgg gttaagccac acttaaacga aagctccggc cagggccgtg cggctgggca
     3061 gcattggccg gggcgcttgc ggcggtcggt gcaggggccg tggaaggctg tcagataaag
     3121 gtgcggcgcc gaaggtggcc cacccggaac cgctgctacg ttcaagggcg gcgcacgcct
     3181 cgcgcagcat cggcggtttg gctaagcttc gagcctcacg gcgtcaagcg aatgcgcggg
     3241 ccggttgcgg gcgacaacgg cgcgaggtcg agcgagccag agaattccca caccgattca
     3301 gggcggaaca cagattcccg aagaggacaa gaccggctct ccgctcgttc aatctgtgtt
     3361 ccgacccgaa tctgtgtgga gtccctcacc gattcggtgc acggcacggg tcgaccgggc
     3421 caaagggtcc ccacaccgat tcagggcgga acacagattc ccgaagggga caaaatcggc
     3481 tctccgctcc tccaatctgt gttccgaccc gaatctgcgt ggagtccccc accgattcgg
     3541 tgcacgacac aggtcgagcg agccagagaa tccccacacc gattcagggc ggaacacgga
     3601 ttcccgaagg ggacaagacc ggctctccgc tcgttcaatc tgtgttccga cccgaatctg
     3661 tgtggagttc cccaccgatt cggtgcacgg cacgtgtcga gcgagccaga gaatccccac
     3721 accgattcag ggcggaacac agattcccga
//
LOCUS       DAOQRO010000337         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26934_length_3750_cov_4.699865, whole genome shotgun sequence.
ACCESSION   DAOQRO010000337 DAOQRO010000000
VERSION     DAOQRO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26934_length_3750_cov_4.699865"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /locus_tag="PKK20_11385"
     CDS             <1..1945
                     /locus_tag="PKK20_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00532.1"
                     /translation="VPGALIDSIEVTELRPRYFQPEESMSSLIGQLAVGDWRLEVVDR
                     RTGATNDIDPELLTWQLDLQFGPPLYPILTLTNGVTYTNTLPRATRYFMVNVPEGTER
                     VLNTLTNLAAAGIDLWFNAKGLPTGEPEYGDFRLLTNVVMDAPGYAIVATNGSWLTSS
                     DLQATNNFAGAPRLEPGRTYYLGVRNDQATTAEFALRVDFFPNENRDICPELDPLPPG
                     GVSAVMPASDDLQYYCYTVPQGAVCVRFHLEPLDGDLNLYIRNARSEPPLRPQPDWFE
                     YAANSPGTNAEVIVVNQRSCVPLFAGHWYLGIENREDRGVQYRLWVEEQHAYTDTPLA
                     ADQPIIATARPGNDTCSYFVFSVTNAVPAVQFDLESFSNPARLLVSRNSRPGPCDSLR
                     DDEAAPGLPTRILICTNDAVPDLRGDWYVAVINRAPTDTPFRLSAGYGLDLLPVLRAG
                     VTITNTIGSTLEGGGCIPDAYRFTVVPNATRALFRLTPLNGDADLALRFGQLPETWLF
                     DYLGGTPGITPEFIEVKTNSVPQPLVPGDWFVRVFNQTQSAVTYLLVAEQYADDDPTG
                     IFLDMPFIAPDGTVTLTWSTYPGLVFRLQYATTIPASSPIPWITIPIDITSPTSFYSF
                     VDDGAFTEPFTSFRIYRLLLVGP"
     gene            2230..2610
                     /locus_tag="PKK20_11390"
     CDS             2230..2610
                     /locus_tag="PKK20_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNV00533.1"
                     /translation="MKVIERLLELQELEMGPKAEAPGSRSIIDALRKEVPEPILAHYD
                     RLRARGKKGVALVRHGVCSGCQMRLATGVHAALLRDEDIAMCDNCARYLLVAPELPPP
                     TPEPPPKKAPVRRRRRKAAPDPSA"
     gene            complement(2776..3444)
                     /locus_tag="PKK20_11395"
     CDS             complement(2776..3444)
                     /locus_tag="PKK20_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV00534.1"
                     /translation="MNADWSDVANPNGVWSYNHGDVPIKTYQTFWWGQAGWGYYSNAD
                     GSILKGSPESGKDHDWLLGDVVMHSLSRNYGGATTFLNVKWTSPADGVVDIAGRAWEA
                     EIYADRDVRWTLSVAGQTVAARGSVQGLYRTDAGAQFAANVTAGSSLQNIPVNVGDVV
                     QFSVMSEQGYGHFVGVEENITLTVVPEPGVGALLVAGGAALGMMRRCRQRESGRGPLA
                     RMVE"
BASE COUNT          706 a         1357 c          995 g          692 t
ORIGIN      
        1 cgttcccggg gctttgattg actcgatcga ggtgaccgag ttgcgcccac gctatttcca
       61 gcccgaggaa tccatgtcga gcctcatcgg ccaactcgcg gtcggggact ggcgcctcga
      121 agtggtcgac cgccggaccg gcgcgaccaa cgacatcgat cccgagcttc tcacctggca
      181 gttggacctc cagttcgggc cgccactcta tcccatcctc accctcacca acggcgtgac
      241 ctacaccaac acgctgccac gcgccacacg ctatttcatg gtcaatgtgc cggaggggac
      301 cgagcgggtg ctcaacacgc tcaccaacct cgccgcggcc ggcatcgacc tctggttcaa
      361 cgccaagggg ttgccgaccg gcgagcccga gtacggcgat ttccggttgc tcaccaacgt
      421 cgtcatggac gcacccggtt atgcgatcgt cgccaccaac ggctcctggc tcacctcgtc
      481 cgacttgcag gccacaaaca atttcgccgg cgcgccccgc ctcgagccgg gccgcaccta
      541 ttatctgggc gttcgcaacg atcaggccac cactgccgag tttgccctcc gggtcgattt
      601 ctttccgaat gaaaaccgcg acatctgccc cgaactcgat ccgctgcccc cgggcggcgt
      661 gagcgccgtc atgcccgcgt cagacgatct tcagtattac tgctacaccg tgccccaagg
      721 agccgtgtgc gtccgattcc acctcgaacc cctcgacgga gacctcaacc tctatatccg
      781 caacgcgcgt tcggagccgc ctctgcgccc gcaacccgat tggttcgagt acgccgccaa
      841 cagccccggc accaacgccg aggtgatcgt tgtcaaccag cgctcctgcg tccccctgtt
      901 cgccggccac tggtacctgg gcatcgaaaa ccgcgaggac cgcggcgtcc aataccggct
      961 ctgggttgaa gaacagcacg cctatacgga tacgccgctg gcagccgatc aaccgatcat
     1021 cgccaccgcc cgcccaggca acgacacctg ctcctacttc gtcttctccg tcaccaatgc
     1081 cgtccccgcc gtccagttcg acctcgaatc attctcgaat cccgcccggc tcctggtcag
     1141 ccgcaacagc cggccgggac cgtgcgacag cttgcgcgac gacgaggccg cccccggcct
     1201 ccccacccgc atcctcatct gcacgaacga tgccgtgccc gatttgcgcg gggattggta
     1261 tgtcgctgtc atcaatcgcg ccccgaccga cacccccttc cggctcagcg ccggctacgg
     1321 cctcgatctt ctgcccgtgc tccgcgccgg cgtcacgatc acaaacacga tcggcagcac
     1381 tctcgaaggc gggggctgca tccccgatgc ctaccgcttc accgtcgtcc ccaatgccac
     1441 gcgcgctctg ttccgcctga ctcccctcaa cggcgatgcc gacctcgccc tgcgtttcgg
     1501 ccaacttccc gaaacgtggc tcttcgacta cctgggtggc acgcccggca tcactcccga
     1561 attcatcgag gtgaagacga acagcgtccc gcagccattg gtgcccggcg actggttcgt
     1621 gcgcgtgttc aaccagaccc agagcgccgt cacctacctc ctcgtggccg agcaatacgc
     1681 cgatgacgat ccgacgggca tcttcctcga catgccgttc atcgcccccg acggcacggt
     1741 gacgctcact tggagcacat accccggcct ggtctttcga ctccagtacg ccacaacgat
     1801 cccggcctcc agcccgatcc cctggatcac gatcccgatc gacatcacgt cgcccacgag
     1861 cttctactcg ttcgtcgacg atggcgcgtt caccgagcca ttcacatcgt tccggatcta
     1921 ccggctcctg ctggtcggac cgtgatcccg ggctccgtcc aactcaacac ctgacgcgcc
     1981 ggcagcgccg aatccggagt tcagcccgca tttctcgcca actgtcaatg tcatctgacc
     2041 ggtcaccgtc acgcttcacc tgaaaagcgg gctcacagac ccggaaatca cacccgcaga
     2101 tcgtccggaa tgccgcggtc gccccactgg tgagatttcc gggttagaga ttcttcacat
     2161 atttctctgg attttcagaa accaaatcgc aacactctct gcctgtcgcg cacacgaggc
     2221 ctcgaagaga tgaaagtcat agagcgtttg ctggaactgc aggaattgga gatgggaccg
     2281 aaagccgagg cacccggaag tcgctcgatc atcgacgctt tgcgcaagga ggtccccgag
     2341 ccgattctgg cgcactatga tcgcctgcga gcccgcggca agaaaggggt cgctctagtg
     2401 cggcatgggg tgtgttccgg ctgccagatg agactcgcca ctggcgtcca cgccgcgttg
     2461 ctgcgggacg aggatatcgc catgtgcgac aattgcgccc gttacctcct cgtcgcgccc
     2521 gagctgccgc caccaacgcc cgaaccgcct cccaagaagg cccctgttcg ccgcagacgc
     2581 cgcaaggctg cgcccgatcc ctctgcctga tccagccctc ctcgatcggg ccctcgatgc
     2641 gcaaaccgcg ccggcatcca ccaggatcgg catcagtcga gcttctggat caggtggcgc
     2701 ctttgttctt tcccggtcct gccttccatc atccacaagg ccgtgcccaa gggcttgcgc
     2761 gccttcatgc ctctcttact ctaccatcct ggccaatggc cctcgccccg attcacgttg
     2821 ccgacagcgg cgcatcatcc ccaaagcggc gccgcccgca accagcagtg ctccgacgcc
     2881 aggctcggga acgaccgtga gcgtgatgtt ctcttcgacc ccgacaaagt gaccgtaccc
     2941 ctgctcgctc atcacgctga actgcacgac gtcgcccaca ttgacgggaa tgttctggag
     3001 gctgctgccg gccgtcacgt tggcggcgaa ttgcgcgccc gcatccgttc gatacagtcc
     3061 ttgaaccgag ccgcgcgcgg ccacggtctg cccggccacg ctcagagtcc aacggacgtc
     3121 gcgatcagcg tagatctcgg cctcccaggc gcgaccggcg atatcaacaa ctccatcagc
     3181 ggggctggtc catttcacat tcaggaaggt tgttgcgcca ccgtaattac gagaaagcga
     3241 gtgcataacc acatcaccca acagccagtc atgatctttg cccgactcgg ggctgccttt
     3301 cagaatgctg ccatcggcat tcgaatagta gccccagccc gcttggcccc accagaaggt
     3361 ctgataggtc ttgatgggca cgtcaccgtg gttgtaggac caaacgccat tcggattcgc
     3421 cacgtcactc caatcggcgt tcaagctgta atgatactgg acgagatcag ccgcccatgc
     3481 gcaagacgcc atcaaccaaa cgatggcggt gccgactgcg aaccggagcg gactcggagg
     3541 ccgagacacg tttctcattc tcataagacg gatggaggtt tgattgttca gtgaagctgg
     3601 gctttaccag caatgcctca agcgtacacg ccctcgaggg gcgcgtcaaa gcagcgaagg
     3661 ccgtggactt tccccgatat tctgcacccc gctcctggaa agcattgaca ttcaacggtt
     3721 taatgcgcat tggacccatg aaccggggaa
//
LOCUS       DAOQRO010000338         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_26936_length_3750_cov_4.282003, whole genome shotgun sequence.
ACCESSION   DAOQRO010000338 DAOQRO010000000
VERSION     DAOQRO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26936_length_3750_cov_4.282003"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..2189
                     /locus_tag="PKK20_11400"
     CDS             117..2189
                     /locus_tag="PKK20_11400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNV00535.1"
                     /translation="MSRDYVLPRFQAPAAPQIDYARELNPQQFAAVTAVPGPALVIAG
                     AGAGKTRTLVYRVAFLIEQGIPPECILLLTFTNKAAREMMQRVRNLLENDLGGLWGGT
                     FHSIGHRILRRHAEAIGYPPNFTILDRDDAADLIRACLADAKVAPQEPLFPKADVLAE
                     IFSLATNMQIGIPETLERHFSSFEPLAEVIVDLQRRYSERKRQAGVVDFDDLLALWLA
                     LLRDHPDVLERMQRRFQFLLVDEYQDTNRIQSDLVDLLAARHDNIMAVGDDSQSIYSW
                     RGANFQNILRFPDRHPNTTVYRIETNYRSTPDILRLANAAIAPNIHQFPKELTAVRPP
                     GAKPALVVCRDGRVQAAFVATRAAELREQGTSLDAMAVLYRSHFHALELQLELTRRQI
                     PFSITSGIRFFEQAHVKDLTAILKLVSNHRDEVAFKRLVRLLPGIGAVVSGRLWAQFA
                     RQLPPPPSASSSVPDGPPPLSPPLIARSFEALASSVPKKASSAWTQLSATLGQLEQER
                     ARGKVSDLIALALDGFYRTHLETTYDNARARIDDLDQLASYGRQFASLHEFLAQLALL
                     SNVEAEGETRPQQDDERIRLTTVHQAKGLEFDVVFLIMLCDGLFPSARSAKSVEGEEE
                     ERRLFYVAITRARNELYLCYPATRSFGVGGAETPQQPSRFLEDLPAELMETWNLHPAA
                     WSEETRTL"
     gene            complement(2266..3123)
                     /locus_tag="PKK20_11405"
     CDS             complement(2266..3123)
                     /locus_tag="PKK20_11405"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNV00536.1"
                     /translation="MNPYHHLVSEYARYAREAKNYPLGGFPPAPKPELPANAPKALIF
                     SPHPDDECIVGGLALRLLREARMHVINVAVTQGSNPARQAERLEELRNACHYLGFGLL
                     QTGPTGLEKVNRKTREGQPAVWQPMVHTIAQILLEHQPRVIFFPHDQDWNSTHEGVHY
                     LVTDALQEVGTRLSCFSVEFEYWGQMDDPNLMVELTATDVADMVTALTFHVGEVKRNP
                     YHLVLPAWMLDNVRRGGELVGGQGQAAPDFVFATLYRLRRWTGTRLERFYDGGRNLSA
                     GVNPDSLFA"
     gene            complement(3133..>3750)
                     /locus_tag="PKK20_11410"
     CDS             complement(3133..>3750)
                     /locus_tag="PKK20_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV00537.1"
                     /translation="FEVANDGEVTALAGSMALGDNAVLGIALGTSTAGGYVTPQGNIT
                     SWLNELAFVPVDYNPGASVDEWSGDFGCGARYFSQQCVGRLVEVAGIPVAAGTPLPEC
                     LKQVQALMASNDERARRIYQTIGTYLGYTIAHFADFYEFRHLLILGRVTSGTGGEVIL
                     DEAKHVLQAEFPELAARVNLHTPDEKEKRHGQAIAAASLPVIPHP"
BASE COUNT          693 a         1285 c         1090 g          682 t
ORIGIN      
        1 ccgaaatccc cgagccgcgc gaggcgttga ttgccgattt gaccggcgac ggcaagaacg
       61 atctggtcgt cgtcgtccat gatcgcattc tggtctatcc ccaggagtag aatgccgtgt
      121 cacgcgacta tgtgctgcct cgatttcagg cacccgccgc gccgcaaatc gactatgccc
      181 gggagctgaa cccccagcag ttcgcggcgg tcaccgccgt gcccggccca gctctggtca
      241 tcgccggcgc cggcgccgga aaaacacgca cgctcgtcta ccgtgtggct ttcctcatcg
      301 aacaggggat tcctcccgag tgcatcctgc tcctcacctt caccaacaag gcggcgcggg
      361 agatgatgca gcgcgtgcgc aacctgctcg agaacgattt gggcggcctc tggggcggca
      421 cctttcactc catcggccac cgcatcctgc gccgacacgc tgaagccatc gggtatccac
      481 ccaacttcac catcctggac cgggatgacg ccgcggactt gatccgcgcc tgcctcgccg
      541 acgccaaggt cgcgccgcag gagcctctct tccctaaagc cgatgtgctc gccgagattt
      601 tctcactggc cacgaacatg cagatcggca tccccgaaac cctcgaacgg catttctcgt
      661 cgttcgagcc actggcggag gtcattgtcg acctccagag gcgctacagc gaacgcaaac
      721 gccaggcggg cgtcgtcgat ttcgacgatc ttctggcgct ctggctggct ctgctccgcg
      781 accacccgga cgtgctcgag cgcatgcagc gccgctttca gttcctcctc gtcgatgagt
      841 accaggacac caaccggatt caaagcgacc tggtcgatct gctggcggcc cgacacgaca
      901 atatcatggc cgtcggcgac gattctcaga gcatctactc gtggcgcggc gccaacttcc
      961 aaaacatcct gcggttcccc gaccggcacc ctaacaccac cgtctaccgg atcgagacca
     1021 attaccgcag cacgcccgat atcctccgcc tcgccaacgc cgccatagct ccgaatatcc
     1081 accagttccc caaggaactc accgccgtgc gcccgccggg ggccaagccg gcgctggttg
     1141 tctgccgaga cggccgggtc caagcagcct tcgtggccac gcgagccgcc gagttgcgtg
     1201 agcaagggac ctcgctcgac gccatggccg tgctctatcg ctcccatttc catgcgctcg
     1261 aactccagtt ggaactgacc cgtcgccaga ttccattcag catcaccagc ggaatccgtt
     1321 tcttcgaaca ggcccatgtc aaggacctga ccgccatcct caaactggtt tcgaatcacc
     1381 gcgacgaagt cgccttcaag cgcctggttc gcctgctccc cggcataggc gccgtcgtgt
     1441 ccggacggct ctgggctcag ttcgcccgcc aattgccccc gccgccgtcc gcgtcctcat
     1501 cggtcccgga cgggccgccg ccgctctccc ctcccctgat tgcccgctcg ttcgaggctc
     1561 tggcgtcatc ggtgcccaag aaagcatcct ccgcgtggac gcaactctcc gcgacactcg
     1621 ggcaactcga acaggagcgc gcgcgaggca aggtctcgga cctgatcgct ctcgcgctcg
     1681 atggctttta caggacacat ctcgagacaa cttacgacaa tgcccgcgcc cggatcgacg
     1741 acctcgacca actcgcctcc tatggccgac agttcgccag cctgcacgag ttcctcgccc
     1801 aactcgccct gctcagcaat gtcgaggccg aaggcgaaac ccgccctcag caggatgacg
     1861 aacgcatccg cctcacgact gtccaccagg ccaagggcct cgaattcgac gtcgtgttcc
     1921 tcatcatgct ctgcgatggc ttattcccct cagcccggtc cgccaagagc gtcgaaggcg
     1981 aggaggaaga gcgacgcctg ttctatgtcg ccatcacgcg cgcccgcaat gagctctacc
     2041 tctgttaccc cgccacacgc agctttggcg tcggcggcgc cgagaccccc caacagcctt
     2101 cgcggtttct cgaggacctg cccgccgaat tgatggaaac atggaatctc caccccgcgg
     2161 cgtggtccga ggaaactcgg acactgtagt tcgtcaggct acggtgcctc acgaggcgtg
     2221 tgaaataacc gctaacccgc ttcagaccgg tccatcgcta agggtctaag caaacagact
     2281 gtcgggattg accccggccg acaggttgcg tcctccatca taaaaccgtt cgaggcgtgt
     2341 gccggtccag cgtcgcaacc ggtacaaggt ggcaaacaca aaatcgggcg ccgcttgccc
     2401 ctggccgccg acgagttctc ctccgcgccg gacgttatcc aacatccaag ccggcaggac
     2461 gaggtgatac ggattgcgct tcacctcgcc cacgtggaac gtgagggcgg tcaccatgtc
     2521 ggcgacgtcg gtggccgtca gttccaccat caggttcgga tcgtccatct gcccccaata
     2581 ctcgaactcg acactgaagc atgagagtcg agtgccgacc tcctgcaggg cgtccgtgac
     2641 caggtaatgc actccctcat gcgtgctgtt ccaatcctgg tcgtgcggga agaagatcac
     2701 gcggggctga tgttcgagga ggatttgggc gatcgtgtgg accatcggct gccagacggc
     2761 gggttgaccc tcacgtgtct tgcgattgac tttctcgaga ccggtcggcc cggtctgaag
     2821 caggccaaag ccgagataat gacaggcgtt cctgagttcc tcgagccgtt ccgcctggcg
     2881 ggcgggattg ctgccttggg tgacggcgac gttgatcacg tgcatgcggg cctcacgcaa
     2941 caggcgcagc gcaagaccgc cgacgatgca ctcatcgtcc gggtgaggcg agaagatcaa
     3001 agccttgggc gcgttggccg ggagctccgg tttcggggcc ggcggaaagc cgcccagtgg
     3061 atagttcttc gcttcccggg cgtaccgggc atattcgctg acaaggtggt gatacgggtt
     3121 catagctgac aattacggat gagggatcac gggaagactc gccgcggcga tggcctggcc
     3181 atgtcgtttc tccttctcgt cgggcgtatg caggttcacc cgggcggcca attccgggaa
     3241 ctcggcctgc agcacgtgct tggcctcatc gagaatcacc tcgccaccgg tccccgacgt
     3301 gacgcgcccg aggatgagca gatgacgaaa ctcgtagaag tcggcgaagt gggcgatggt
     3361 gtaaccgagg taggtgccaa tggtctggta gatgcgacgg gcgcgctcgt cgttcgatgc
     3421 catcagggcc tggacctgtt tgaggcattc cggcaggggt gtcccggcgg ccaccgggat
     3481 gccggcgacc tcgaccagcc gacctacaca ttgttgggag aaatacctcg cgccacagcc
     3541 gaaatcgccc gaccactcgt ccaccgaggc gccgggattg tagtcgaccg gcacaaacgc
     3601 cagttcgttg agccaggagg tgatgttgcc ttgcggggtg acatagcccc cggcggtgct
     3661 tgtgcccagc gcaatgccca gcacggcgtt gtcgccaagc gccatcgagc ccgccagcgc
     3721 agttacctcg ccgtcattcg ccacctcgaa
//
LOCUS       DAOQRO010000339         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_27159_length_3731_cov_4.613983, whole genome shotgun sequence.
ACCESSION   DAOQRO010000339 DAOQRO010000000
VERSION     DAOQRO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27159_length_3731_cov_4.613983"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            546..1784
                     /locus_tag="PKK20_11415"
     CDS             546..1784
                     /locus_tag="PKK20_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV00538.1"
                     /translation="MLVLVGLMVLALPGCTTSHGKRVGYRYNPDYGVATLQFQQTLMG
                     QANGLIPGNRARLLNNGDAFFPAMLEAIRNARHSVNIELYIFARGRIGEQMTAALCAK
                     AREGVQVRVLVDAMGSRTGGLDRRMMAAGVHFRKYKPVTIPTITKTGDRTHRKIVTID
                     GRIGFTGGFAVDDRWQGNVRNSREWREVVLEIEGPVVQQFQRTFCENWLYATGEVIDG
                     PGQFPDPVPVGDLKAQVVGSTRTSQLSMAKLHHYLPIQAARERVWIANAYFLPDRDFR
                     RALCAAARRGADVRLLLPGRHNHLKTVRYASRRDYRRFLEAGVKVYEYRPSMLHTKMM
                     VVDSIWTSIGSVNFTSRSMKAQAEANLAIYDRGFARQAEAVIEEDFRDSDCMDLASWR
                     RRGLCERMKELYFSLYKRLF"
     gene            1871..2398
                     /locus_tag="PKK20_11420"
     CDS             1871..2398
                     /locus_tag="PKK20_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNV00539.1"
                     /translation="MNDAFAGFDRDEASAFIGSFDRATGVGASAISKHGGGHKVPVFS
                     QFVQLLDLLKPVLSARKWPVFCLTGDTENRCQLVSTFHESAGNAVFLISLKAGGFGLN
                     LTAASYVVLFDRWWNPAVETQAIDRTHWIWQSNTVIGYRLLSIRGRALAAHAASDKPG
                     ASEPEALDDRTPSEQ"
     gene            2470..>3731
                     /locus_tag="PKK20_11425"
     CDS             2470..>3731
                     /locus_tag="PKK20_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00540.1"
                     /translation="MQLPTTLTRRAFLARGSLAAGATLALPRLTLAQGSPNAKVNVGF
                     VGVANRAGDNLNEVARLADSVNVVALCDVDAKYLGAAKEKHPGAQTYADFRRLIDQKG
                     IDAVVVSTPDHTHAVACAAALRTGRHVYCEKPLTRTISEARVVANLARQHRRVTQLGT
                     QIHATANYRRVVELIQSKAIGLVREVHVWVAASYGGMERPTDTPPVPAGFDWDLWLGP
                     VEFRPYHPDYAPFKWRNWWAFGGGSLADFGCHFMDLPHWALDLQHPLSAEPVDGPPVH
                     PESTPPWAIVRYQHPARRSSAGETLPPVALTWYHGSKQPSFLTQEQAQYFRSGVMFVG
                     ERGNLLADYTRRQLLPEKDFADFKPPAPSIPDSIGHHKEWIEAIRSGGPTTCNFDYSG
                     ALTETALLGNVAYRLGRKIEWDARRLVS"
BASE COUNT          681 a         1145 c         1196 g          709 t
ORIGIN      
        1 tcgattcctc cgatcgatac tcctcgtcgc ccgccaggag aacgatgtgt ttgccgttgg
       61 ccttcccggc gggcggttcg tagacgaccc aatgggcggc gttgctattt tggctcagac
      121 aacttagaac catcagaagt gcaaggcgtt tcatgcggct gtgtatagcc tgcgttcccg
      181 tcggaggcga caggaaatgc gcatcctcga aaagagacgg tgttggcgaa tcgcccgggc
      241 cagcgggccc gaatcgagga ccggaccccc tgaccgatgc cacatccggg ctccagtgcg
      301 gccccgctcg gagcgctcgc ccgcctgcac cccaaaatga agactgcagg cggcagactg
      361 aaaggcgttg ccaccaaaag cccgagacga ttgactatcc ccccacggtg cgacccgaaa
      421 cgaagctgag cataacgaat cgattccgca gtccccgcgc tgttcccggc cggagatccg
      481 accgcgcgaa gcggtgcagt catgcgcccc agaatctacc cagcccgcga cgaagatggg
      541 gggtcatgct cgtgttggtg gggttgatgg tgctcgcgct cccgggctgt acgacttcac
      601 acggcaagcg ggtcgggtac cggtacaatc ccgactacgg cgttgccacc ctccaattcc
      661 agcagacgct gatgggtcag gccaacgggc tgattccagg caaccgggcg cggctgctga
      721 acaatgggga cgccttcttt cccgcgatgt tggaagcgat tcggaacgct cgacacagcg
      781 tgaacatcga gctctacatc tttgcccggg ggaggattgg cgagcagatg acggcggctt
      841 tgtgcgcgaa ggcgcgcgaa ggcgttcagg tgagggtttt ggtggatgcc atgggatcgc
      901 gcacgggagg gcttgaccgg cggatgatgg ccgcgggggt gcattttcgc aagtacaagc
      961 cggtgaccat accgaccatc acgaagaccg gagaccggac gcaccgcaag attgtgacca
     1021 ttgacgggcg gatcgggttc acgggcggtt ttgccgtgga cgaccgatgg cagggaaacg
     1081 tgcgcaattc gcgcgagtgg cgggaggtgg tcctggagat cgaggggccg gtggtgcaac
     1141 agttccagag gacattctgc gagaattggc tttacgccac cggcgaggtc atcgacgggc
     1201 cggggcagtt tcccgatccg gtgcctgtgg gcgatctgaa ggcgcaggtg gtgggaagca
     1261 cgcgcacatc ccagttgtcg atggccaagc tccaccatta cctcccgatc caggcggcgc
     1321 gcgaacgcgt ctggatcgcg aacgcatact tcctgccgga tcgcgacttc cgccgggcct
     1381 tgtgcgccgc ggcccgacgc ggggcggacg tacgcctcct gttgccgggc cgtcacaatc
     1441 acctgaaaac cgtgcggtac gcatcgaggc gggattaccg acggtttcta gaagcggggg
     1501 tgaaggtgta cgagtacagg cccagcatgc tgcacaccaa gatgatggtc gtcgacagca
     1561 tctggacctc gatcggatcg gtcaacttca cctcacgttc gatgaaggcg caggccgagg
     1621 ccaacctggc catctacgac cggggtttcg cccggcaggc cgaggcggtc atcgaggagg
     1681 atttccgcga ctcggactgc atggatctcg cctcatggcg gcggcgcgga ctgtgcgagc
     1741 gcatgaaaga actctatttc agcctctaca aaaggctgtt ctaacctcga tctatgaaac
     1801 gtgctctaaa caaatcgagt ctctttgctc cgctgaagag gccgacgatg caactggatt
     1861 ctccgacaga atgaacgatg cgttcgcagg attcgaccgg gacgaggcct ctgcctttat
     1921 cgggtccttc gatcgtgcca cgggcgtcgg ggccagcgcc atttccaaac atggaggggg
     1981 gcacaaggtg ccggtctttt cgcagttcgt gcaattgctc gatctgctca aaccggtgtt
     2041 gtcggcccgc aaatggcccg tgttctgtct caccggcgac accgagaatc ggtgccaact
     2101 ggtgagcacg ttccatgaaa gcgccggaaa cgcggtgttt ctgatctcgc tgaaggccgg
     2161 cggcttcggt ctcaacctca cagcggccag ttatgtcgtg ctgttcgatc gctggtggaa
     2221 cccggccgtc gagactcaag ccatcgaccg cacacactgg atttggcaat ccaacacagt
     2281 gattggctac cgcctgctca gtatccgggg tcgggcgctg gcggcgcatg cggcgtccga
     2341 caaacccggc gcatccgaac ccgaggcgct cgacgaccgc actccctcag aacaatgact
     2401 gtggctcttg catttgcagt cgtgatttcc tctttattcg cagccgattt gttgctagag
     2461 gattctccca tgcaattgcc tacgactctg actcgtcgcg cgtttctcgc tcgaggttcg
     2521 ctggccgcgg gcgccacgct cgctcttccg cggctgacgc ttgcccaagg ttcccccaac
     2581 gccaaggtca atgtcggctt tgtcggggtt gccaatcgcg ccggcgacaa cctgaacgag
     2641 gtcgcccgcc tggcggattc ggtcaatgtg gttgcgctat gcgatgtgga cgccaagtac
     2701 ctgggggccg cgaaggagaa gcatccgggg gcgcagacct atgcggactt tcggcgtttg
     2761 atcgatcaga aggggatcga tgccgttgtg gtgagcacgc ccgatcacac gcatgcggtt
     2821 gcgtgcgccg ccgcgctgcg gaccggacgg catgtgtatt gcgagaaacc gctgacccgc
     2881 acgatttccg aggcgcgcgt tgtcgcgaat cttgcgcggc agcatcggcg cgtgacccag
     2941 cttgggactc aaatccacgc cacggcgaat taccgaaggg tggttgaact gatccagtcc
     3001 aaggcgatcg gtctggtgcg cgaggttcac gtctgggtgg ccgccagcta cggcggcatg
     3061 gaacgtccaa ccgacacccc tccggtcccg gcgggcttcg actgggacct ctggctcggg
     3121 ccggtcgagt tccgtccgta ccatcccgac tatgcgccgt tcaagtggcg gaactggtgg
     3181 gcttttgggg gcggatcgct tgcggatttt ggctgtcact tcatggatct cccgcattgg
     3241 gcgctcgatc tgcagcatcc gctgtccgcc gaaccggtgg atggcccgcc tgtgcacccc
     3301 gagtccaccc cgccttgggc gatcgttcgc taccagcatc cggcgcggcg gagttccgcg
     3361 ggcgagactc tgcccccggt tgcgctcacg tggtatcacg gcagcaagca gccgtccttc
     3421 ctgactcagg agcaggcgca gtacttccgc agcggcgtca tgttcgttgg cgagcgcggc
     3481 aacctgctgg cggactacac gcggcggcag ttgctgcccg agaaggattt cgccgatttc
     3541 aagccgcccg caccgtccat cccggactcg atcggccatc acaaggaatg gatcgaagcg
     3601 attcgctccg gcggccccac aacctgcaac ttcgattact ccggcgcctt gaccgagaca
     3661 gccctcctgg gcaacgtggc ctatcgcctc gggcgcaaga tcgagtggga tgcgcggaga
     3721 ttggtctcga t
//
LOCUS       DAOQRO010000340         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_27262_length_3722_cov_4.407690, whole genome shotgun sequence.
ACCESSION   DAOQRO010000340 DAOQRO010000000
VERSION     DAOQRO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27262_length_3722_cov_4.407690"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKK20_11430"
     CDS             <1..1098
                     /locus_tag="PKK20_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV00541.1"
                     /translation="ARPAHGRLRQKLAEIQAWNETSDLNRVLPGDPALGIITSGVSYA
                     HVREAAPKASVLKLGLTYPLPLRRVAEFARGVVRCVVIDEGDPWLVDALRAAGIRVEG
                     KPDMYRFGELNVPRVRRILEGDVSPESKPPPGKPPQLCDACQYRVVFDTLRRHNCIVA
                     GDIGCYTLGVLPPFEAMDSCVCMGASIGVGLGLRHVLPPDEARRVVSVIGDSTFVHSG
                     ISGLVEMVYNPPPGGHVLIILDNSTTAMTGHQEHPGTGRTLDHEPTNKLVIEDLASAL
                     GVRRVHVVEPRVGSEDFERMLLECLASGELCVLIARRPCILIAKRLKELEQRHIAPKS
                     PPASTGVAAEATCCSSAVPAGAGADRLESGI"
     gene            1101..1604
                     /locus_tag="PKK20_11435"
     CDS             1101..1604
                     /locus_tag="PKK20_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV00542.1"
                     /translation="MTATVTNIVVAGLGGQGVLKASDVLSDALLRAGHDVKKSEVHGM
                     AQRGGSVRSDVRYGPEVFSPMVSPGEADYLVALDSSQVDVCRHLLRQGGIVIAPDAIE
                     AAALPNRRSLNVALTGALSVHLDLPAELWTAALHANFRQDLWAANEQAFRLGRLSQNG
                     SAKHAST"
     gene            1672..2997
                     /locus_tag="PKK20_11440"
     CDS             1672..2997
                     /locus_tag="PKK20_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV00543.1"
                     /translation="MSKNYQPGPFHPASAMDYLPLPQLRDLQFQRLRAMVQRACDRVA
                     LFRQRMEARSLKPAHLETLDDIAKLPFTVKTDLRDTYPFGLFASPLTEIVRFHASTGT
                     TGKPIVVAYTQEDLNDWTQVMLRSFAACGVHRGDIVHNAYGYGLFTGGLGAHYGAEAL
                     GASVIPISGGNTDRQVMVLRDFGATAICCTPSYFLHLIERAAGMGINFKDLPLRAGIF
                     GAEPWTESMRQRIEAESGIKAYDIYGLSEIIGPGVAMECLHQAGPHIFEDMFYPEIID
                     PETCELLPDGAEGELVLTTLCKRAMPMIRYRTRDITSLNSEPCACGRTLRRIRRIGRR
                     SDDMLIIRGVNVYPSQIESALLAVEGAQPHYQIVVDRQKGLDVLEVQVEVTAELFSDT
                     VGGLEKLQAKLSRSVESVTGLRAEVRLVAPHTLQRSEGKAKRVIDKRKM"
     gene            3028..3468
                     /locus_tag="PKK20_11445"
     CDS             3028..3468
                     /locus_tag="PKK20_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV00544.1"
                     /translation="MILKQLSIFLENKPGTLSAPCRALADAGINIQTFALADTQKFGI
                     LRLVVHEWEKARRVLLDGGWTVKVTDVVAIEVPDAPGGLASLFEVFERGSINVEYMYP
                     FTAKQEGKGLMLFRFDDPDAAVKALQANKVSVVGTVELFKRLGN"
     gene            3568..>3722
                     /locus_tag="PKK20_11450"
     CDS             3568..>3722
                     /locus_tag="PKK20_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00545.1"
                     /translation="MPDPTPDSIKPKYKRVLLKLSGEALGGEAGTGICPEVVHHMAGQ
                     IREVRELG"
BASE COUNT          683 a         1265 c         1152 g          622 t
ORIGIN      
        1 gcccgtccgg cccacggccg cctgcgccag aagctggctg agatccaggc ctggaacgag
       61 acctcggatt tgaaccgtgt gttgcccgga gacccggccc tgggaatcat cacctccggc
      121 gtttcctacg cccatgtgcg cgaagccgcg cccaaagcct ctgttctcaa gctcggtctc
      181 acctacccgc tcccgctccg ccgcgtggcc gagttcgccc gcggcgtggt ccggtgcgtg
      241 gtgatcgatg agggcgatcc gtggctggtc gatgcgttgc gcgctgccgg catccgcgtc
      301 gaaggcaagc cggacatgta ccgcttcggc gaactcaacg tgccgcgcgt ccggcgcatc
      361 ctcgaaggcg atgtcagccc ggaatccaag ccgcctcccg gcaaaccgcc ccaactgtgc
      421 gacgcctgcc aataccgggt ggtgttcgac acactccgcc gccacaactg catcgtggct
      481 ggggacatcg ggtgctacac gctgggcgtg ctgccgccgt tcgaggcgat ggattcgtgc
      541 gtctgcatgg gcgccagcat cggcgtcggc ctgggcctgc gccacgtgct gccgcccgac
      601 gaagctcggc gcgttgtcag cgtgatcggc gacagcacct tcgtccacag cggcatcagc
      661 gggctggtcg agatggtcta caatccgccc ccgggcggcc acgtgttgat catcctggac
      721 aactcgacca ccgcaatgac cggccaccag gaacacccgg ggaccggacg cacactcgat
      781 cacgaaccca ccaacaagct ggtcatcgag gacctggcca gcgccctcgg cgtccgccgc
      841 gtccatgtgg tcgaaccccg cgtcggatcg gaggattttg aacgcatgct gctcgagtgt
      901 ctggcctccg gggaactgtg cgtcctcatc gcccgccgcc cgtgcatcct gatcgccaag
      961 cgcctcaagg agttggagca acgccatatc gctcccaaat cgccgcccgc gagcacggga
     1021 gttgccgccg aggccacctg ctgctcgagc gccgttccgg cgggcgcagg cgcggatcga
     1081 ctcgagagcg gaatctgact atgacggcga cggtaaccaa cattgttgtg gccggcctgg
     1141 gcggccaggg cgttctcaaa gccagcgatg tgctcagcga cgccctgctg cgcgccggac
     1201 acgatgtcaa gaagagcgaa gtccacggga tggcccagcg cggcggatct gtccgaagcg
     1261 atgtccggta cggccctgag gtgttcagcc ccatggtctc gcccggagag gccgactacc
     1321 tggttgcgct cgattcgagc caggtggatg tctgtcgcca cctgttgcga cagggcggca
     1381 ttgtgatcgc gcccgacgcc atcgaggccg ccgcgctccc caatcgacgc agtttgaatg
     1441 tcgctctgac gggcgccctg agcgtgcacc tcgacttgcc cgcggaactc tggacggcgg
     1501 ccttgcacgc caatttccgc caggacttgt gggcggcaaa cgagcaggcg tttcgactcg
     1561 gacgcctcag tcagaacggc tcagcaaaac atgcatcgac ctgaacccaa cgcgcagacc
     1621 ggggctgcga ccgtctcctg gccgcgccct cccgatcgca actcaaccgc catgagcaag
     1681 aactatcaac ccggcccgtt ccatcctgcg agcgcgatgg attatttgcc attgcctcaa
     1741 ctgcgcgatt tgcagttcca acgcctccgc gcaatggtac agcgggcctg cgatcgggtg
     1801 gccctgttcc gccagcggat ggaggcccgc tcgttgaagc ccgcccacct cgagacgctc
     1861 gatgacatcg ccaagctccc cttcacggtg aagacggatt tgcgcgacac gtatccgttc
     1921 gggctctttg cgagcccgtt gacggaaatc gtgcgtttcc acgcctcgac tggcacgacg
     1981 ggcaagccga ttgtcgtggc gtacacgcaa gaggacctga acgactggac gcaggtgatg
     2041 ctccggagct tcgcggcgtg cggggtgcat cgcggcgaca tcgtccacaa cgcctacggc
     2101 tacggcctgt tcaccggcgg actgggggcg cactacgggg ccgaggcgct cggcgccagc
     2161 gtcatcccaa tctccggagg caacaccgat cgccaggtca tggtgctccg cgacttcggc
     2221 gcgaccgcca tctgctgcac cccaagctac ttcctccacc tcatcgagcg cgccgccggc
     2281 atgggcatca acttcaagga tctccccctc cgcgccggca tcttcggcgc cgagccctgg
     2341 accgagtcga tgcgccaacg catcgaggcg gaaagcggca tcaaggccta cgacatctac
     2401 gggttgtccg aaatcatcgg ccccggcgtg gcgatggagt gcttgcacca ggcgggtccg
     2461 cacattttcg aggacatgtt ctacccggag attatcgatc ccgagacgtg cgaactgctc
     2521 cccgatgggg ccgagggcga gctggtcctg accacgctct gcaagcgcgc catgccgatg
     2581 atccgttacc ggactcgcga catcacatcg ctcaactcag agccgtgcgc ctgcgggcgg
     2641 acgctgcggc gcatccggcg catcgggcgg cgcagcgacg acatgctgat catccgcggc
     2701 gtgaatgtct atccatcgca gatcgagtcg gctctgctcg ccgtcgaggg cgcccaaccg
     2761 cattaccaga ttgtagtcga ccgccagaag gggctcgatg tgctcgaagt gcaggtcgaa
     2821 gtcacagccg agctgttcag cgacacggtc ggaggactcg aaaaactcca ggccaagctg
     2881 agtcggtcgg tggaaagtgt caccggcctg cgcgccgaag tgcgtctggt cgcccctcac
     2941 accctccagc gcagcgaagg caaggccaaa cgcgtgatcg acaaacggaa gatgtaaacc
     3001 gggtcccgtc tcaagaaagg catgctcatg atcctcaagc aactctcgat cttcctcgaa
     3061 aacaaacctg ggacgctgag tgcgccctgc cgcgccctcg ccgatgcggg catcaacatc
     3121 cagacattcg ccctggccga cacccagaaa ttcggtatcc tccgcctcgt cgtccacgaa
     3181 tgggaaaagg ccaggcgggt cttgctcgat ggcggatgga cggtgaaagt caccgatgtg
     3241 gtggcgatcg aggtgccgga tgcccctgga ggtctggcga gtctcttcga ggtgttcgag
     3301 cgcggcagca tcaacgtcga gtacatgtac ccgttcaccg ccaaacagga gggcaaggga
     3361 ctgatgctgt tccgcttcga cgatccggac gccgcggtca aggccctcca ggcgaataag
     3421 gtcagtgtgg tcggcaccgt ggaactcttc aaacgcctcg gcaactagcc cgcatgtttc
     3481 atggacttgt gaccgcggcg aagctgaaaa ctcgaagacg cggatcgctg cgggctagaa
     3541 cggagcgatc ccccagccat ctgtcgcatg cctgatccaa cgcccgattc catcaagccc
     3601 aagtacaagc gcgtcctgct caaactcagc ggcgaagccc ttggcggcga agccggcacc
     3661 ggcatctgcc ccgaggtcgt ccaccacatg gccggccaga tccgcgaggt ccgcgaactg
     3721 gg
//
LOCUS       DAOQRO010000341         3681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_27691_length_3681_cov_5.295643, whole genome shotgun sequence.
ACCESSION   DAOQRO010000341 DAOQRO010000000
VERSION     DAOQRO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27691_length_3681_cov_5.295643"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..2771
                     /locus_tag="PKK20_11455"
     CDS             117..2771
                     /locus_tag="PKK20_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV00546.1"
                     /translation="MADRIRLGKVACLLVIILMPAGFVLDLCLYPDEAPIFLVARLVC
                     SVLVGGLLALHYTELVRQWIRPVSWLIALLPALFISGMIAVNEGANSPYYAGLNLILL
                     ATNVVVHWNLRESLLTSALIVALYVMAIAVNASPPVPGQPYAYLFFMIETAVIVITGN
                     YFYNRLRRREFEAQHALQLSQQNLEHSNKRLLELDELKGRFFANISHELRTPLTLILA
                     PLENLRQHPQVQRDPPLRDCIQTMHANSMRLLKLINDLLDLVRLDVGQMRLHRQAIDV
                     KPFLEGTLNAVRQFSEDRRLRLALTINSGLEKVVADPDKLEKVFLNLLFNAMKFTPAG
                     GMIRVQASADGGDALFEIIDTGVGMSPESVKHLFSRFWQADTSSQRKYQGVGIGLALV
                     KELVEAHAGSVTGESQLGQGTTFRVRLPLDAQPDADEPQADASATPAEPEPQLQAAAT
                     EAHTNWLHQLHRRAELFGNPTPLKDSLQHGSPPSRTGKPRVLIVDDEPDMLRFLRTLL
                     DAHYEVIEAADGDQALTLAAQYLPDVIVCDFMLPHKDGLAVCHELRSRELTRAIPVIV
                     LTARADEATKLSALEAGASDFLSKPFSLAELHVRVKNLADGYRAAKALARQKQTLEAT
                     LEELHDTEAQLIQAAKMASLGRMSAGIIHEINNPLNYALTALSVLSVEARHLPPDNRG
                     EFQETLKDVQDGLQRVGKIIADLREFTHPRGGDVETIEVNHAIEGALRFLKAEVAGKV
                     EIDNRVPDGFAVRAVASKLMQVFVNLVQNSVDALREKTFPEGTKPQIRFEAAIEHGAK
                     IVKVWDNGPGIPASQISKVFDPFYTTKEVNQGTGMGLSICYRLLQEVGAHITVRSEPD
                     VCCEFCIAFPDPANDQIELSTTSTTIAA"
     gene            2768..>3681
                     /locus_tag="PKK20_11460"
     CDS             2768..>3681
                     /locus_tag="PKK20_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV00547.1"
                     /translation="MNTLYDYKRFAILYVDDEEKSLKYFAKAFGDTFRIFTAQNAADG
                     YRILADHQDEIGLIMSDQRMPGEQGVQFLERARKLQPRIIRLLATAFTDLDAAIAAVN
                     NGAIYKYITKPWDIPNLEATLKRGLEFFMLQIERDLLLREKLSSLHRVLIADRILSLG
                     VLAAGLGHRLRNTIEAVQRFLDLAPQMLREEQIDLEQLRNPNFWQDFHRNVKEQVTTA
                     FRMLDDLSSETARPSRFDTETRLHDAVDSAARGLAESLAQKRIQVLNNVPNDLPPLRV
                     DGERFPKLFDLLLRDELANLPDGASVTF"
BASE COUNT          754 a         1290 c          979 g          658 t
ORIGIN      
        1 caccaaagcc gatgccaccg gtgtcaacat ctacctcgaa gtccgccgcc ccgaacgcga
       61 ctgacgttgc cgctcggccc gacccgagtg tgctgatgga cttcgaggcg aaagacatgg
      121 ccgaccgaat ccgcctgggc aaggtggcat gcctgttggt gatcatcttg atgccggctg
      181 gctttgtcct ggacctctgc ctgtatccgg acgaggcgcc gatcttcctg gtggcgcgcc
      241 ttgtttgctc cgtgcttgtg ggcggcttgc tcgccctcca ttacacggaa ctggtgcggc
      301 agtggattcg acccgtaagc tggcttattg ccctgctgcc cgccctcttc atctccggca
      361 tgatcgccgt caacgaaggg gccaactccc cctattacgc gggcctcaac ctgatcctcc
      421 tggccaccaa cgtggtcgtg cactggaacc tccgcgagtc gttgctcaca tcggccctga
      481 tcgttgccct ctacgtcatg gccatcgccg tcaacgcctc ccctcccgtg cccggacagc
      541 cttacgccta cctgtttttc atgatcgaaa cggcggtgat cgtcatcacc ggcaactact
      601 tctacaaccg cctgcggcgc cgcgaattcg aggcgcagca cgccctccaa ctcagtcagc
      661 agaacctcga gcatagcaac aagcgcctgc tggaactcga cgagttgaag ggacgcttct
      721 tcgccaatat cagccacgaa ctgcgcacgc cgctcacact catcctcgcc cccctcgaga
      781 atctgcgcca gcatccgcaa gtgcaacgcg atccgcccct ccgggattgc atccaaacca
      841 tgcatgccaa cagcatgcgg ctgctcaagc tcatcaacga cctcctcgat ctggtccgac
      901 tcgatgtggg tcagatgcga ctgcaccgtc aggcgatcga tgtcaaaccg ttcctcgaag
      961 gaacactcaa tgccgtgcgc cagttctccg aagatcgcag gctgcggctc gccttgacga
     1021 tcaactccgg actcgaaaaa gtcgtcgccg acccggacaa actcgagaag gtgtttctca
     1081 acctgctctt caacgccatg aaattcactc cggcgggcgg gatgatccgc gttcaggctt
     1141 cggccgatgg aggcgacgcc ctcttcgaga tcatcgacac cggcgtgggc atgagccctg
     1201 aaagcgtcaa gcacctgttc agccgcttct ggcaggccga cacctccagc cagcgcaaat
     1261 accagggtgt cggcatcggc ctcgccctgg tcaaggaatt ggtcgaggcc cacgcagggt
     1321 cggtgaccgg cgagagtcaa ctcggtcaag gcacaacctt ccgcgtccga ttgcccctcg
     1381 acgcccaacc cgacgccgac gagccccaag ccgacgcatc cgccactccg gccgagcccg
     1441 agccccagct tcaggccgcc gctaccgagg cccataccaa ctggttgcac caactccacc
     1501 ggcgcgctga attgttcggc aatcccaccc ctctgaagga ctcgctgcag cacggctcac
     1561 cgcccagccg gaccggcaaa ccccgcgtcc tgatcgtcga tgacgaaccc gatatgctgc
     1621 ggtttctgcg aacgctgctg gatgcccatt acgaggtgat cgaggccgcg gatggggatc
     1681 aggccttgac cctcgccgcg cagtacctgc ccgacgtgat cgtctgcgac ttcatgctgc
     1741 cgcacaagga cggcctcgcg gtctgccatg agctgcgcag ccgggaattg acgcgcgcca
     1801 tccccgtcat cgtcctcacc gcgcgagccg acgaggcgac aaaactcagt gccctcgaag
     1861 ctggggcgag cgatttcctc tcgaagccgt tctcgctcgc cgagcttcat gtgcgggtga
     1921 agaatctggc cgacggctac cgcgcggcca aagcgctggc gcgtcagaag cagacactcg
     1981 aagcaaccct cgaggaactg cacgacacgg aagcacagtt gattcaggcg gcaaaaatgg
     2041 cctcgctcgg acgcatgagc gccggcatca ttcacgaaat caacaatccc ttgaactatg
     2101 ccctgaccgc cctgagcgtc ctgtcagtcg aggctaggca ccttccgccc gacaaccgtg
     2161 gcgagttcca ggagactttg aaggatgtcc aggatggcct ccaacgcgtc ggcaaaatca
     2221 ttgcggacct gcgcgagttc acccatcctc ggggaggcga tgtcgagacc atcgaggtga
     2281 accatgccat cgagggagct ctgcgattcc tcaaggccga ggtggcgggc aaagtcgaaa
     2341 tcgacaatcg ggtcccggat ggttttgcag tcagagccgt cgcctccaaa ctgatgcaag
     2401 tgtttgtcaa cctcgtccag aactccgtgg acgcacttcg cgagaagaca ttcccggagg
     2461 gaacaaagcc gcaaatccga ttcgaagccg caatcgagca cggcgccaaa atcgtcaagg
     2521 tctgggacaa cggccccggc atccccgcct cgcaaatctc gaaggtcttc gatccgtttt
     2581 acaccaccaa ggaagtcaac cagggcacgg gcatgggact gagcatttgt taccgcctcc
     2641 tacaggaggt gggcgcccac atcaccgttc ggagcgaacc cgatgtctgc tgcgagttct
     2701 gcatcgcttt ccccgatcct gccaatgacc agatcgagtt atccacgacc tccactacaa
     2761 tcgccgcatg aataccctct acgactacaa gcgctttgcg atcctctacg tcgacgacga
     2821 ggaaaagtcg ctcaagtact tcgccaaggc cttcggcgac acattccgca tcttcaccgc
     2881 tcagaatgcc gccgacggct accgaatcct ggcggatcat caggacgaaa tcggcctgat
     2941 catgagcgac cagcgcatgc ccggcgaaca gggcgtccag ttcctcgaac gcgcgcgcaa
     3001 actccagccc cgcatcatcc gcctgctcgc caccgccttc acggacttgg atgccgccat
     3061 cgccgccgtc aacaacggcg ccatctataa gtacatcacc aaaccgtggg acattcccaa
     3121 cctcgaggcc acgctgaaac ggggtctcga gttcttcatg ctgcagatcg aacgcgatct
     3181 gttgctgcgc gagaaactct ccagcctgca cagggtcctg atcgccgatc ggattctcag
     3241 cctgggcgtt ctcgccgccg gcctcgggca tcgcttgcgc aacaccatcg aggccgtcca
     3301 acgattcctc gacctcgccc cgcagatgct ccgcgaagag cagatcgatc tggaacaact
     3361 gcgcaatccc aacttctggc aggattttca ccgcaacgtc aaagaacagg ttacgacagc
     3421 cttccgcatg ctcgacgacc tgtcctcaga aacagcacgc ccgtcccggt tcgacaccga
     3481 gacccgcctg cacgatgccg tcgactccgc cgcgcgcggc ctcgccgagt cgctggcgca
     3541 aaaacgcatc caggtcctca acaatgtgcc gaacgacctc ccccccctcc gagtcgatgg
     3601 cgagcggttc ccaaagctct tcgacctcct cctgcgcgac gagttggcaa acctccccga
     3661 cggcgcctca gtcaccttcg a
//
LOCUS       DAOQRO010000342         3681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_27693_length_3681_cov_4.918919, whole genome shotgun sequence.
ACCESSION   DAOQRO010000342 DAOQRO010000000
VERSION     DAOQRO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27693_length_3681_cov_4.918919"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PKK20_11465"
     CDS             <1..1252
                     /locus_tag="PKK20_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNV00548.1"
                     /translation="DLRRFIFERWADVARRYGFREYDGPPLEPLELYTTKSGAEIVSQ
                     LYNFIDKGGREVAMRPEMTPTLARMASAQERQYKKPIKWYAIPQLFRYERQQKGRLRE
                     HFQFNADIVGEAEPAADAELIALLIDSLRALGLTASDFVVRLSSRNAWQDFFSRHCSE
                     SSRACEFYQVIDKLEREPPEVSAAKLDALGFALDDVQAFITAGRPTDELRSILDNLAA
                     RGLGAFVKVDYGVIRGLDYYTGPVFEAFDLKGEFRAIAGGGRYDNLIKIMSGGRVDLP
                     ALGFGMGDVVLAELLKARGLTPDAKPRLAAFVVIENEALRADSLALIQRLRDANSSVD
                     YTLVPARSDKQFKRALELGARTTVRLERTPSGDLIAKVKDLATRIERAVAPDDVAGTV
                     ANVEDSHRSMQSAHSAQSDLRAEL"
     gene            1274..2827
                     /gene="xylB"
                     /locus_tag="PKK20_11470"
     CDS             1274..2827
                     /gene="xylB"
                     /locus_tag="PKK20_11470"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNV00549.1"
                     /translation="MRTLILGIDSGTQSTKALLLDVAKKRVVGTGSAPHCLLPGLPPG
                     AKEQRPETWRVATTKAIKAAMKAARAKPAEVRAIGVSGQQHGFVPLDAQGQVIRPAKL
                     WCDTSTAEECDAIMSALGGLEATIGALGNAVLPGFTASKILWLKRHEPRNFARLSTIL
                     LPHDYLNFWLTGRAVMEYGDASGTALLDVRTRRWCAKALEAIDPDLVSKLPPLHRSDE
                     PAGTLQASTAKALGLNPGVLVSAGGGDNMMGAIGTGNTRPGVITASFGTSGTIYACAQ
                     NPVVDPRGEIAAFCDSTNRWLPLLCTMNVTVATEMVRNDFGMSHEAFEKAAAAAPAGS
                     DGLLLLPYLEGERTPNVPSGTGVFFGVAPRTFSAPHFARAAIEGVTLGMNYGLRRLTE
                     LGVKPAQMRATGGGANSRLWRQVMADVFDAEVVTLKVGEGAAYGAALQAFWCWRLQRG
                     EKVSIAEITDAFVALDRKGIAQPNKARVAHYRELQALQDELSRSLRGAFDTHRALVTV
                     RKPVSSSRH"
     gene            2979..>3681
                     /locus_tag="PKK20_11475"
     CDS             2979..>3681
                     /locus_tag="PKK20_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00550.1"
                     /translation="MLPRSRRRLFRLVTVVLAPLLALALIEGSLRLTGYGYPTGFFRR
                     GMAEGRAVLLDNDKFGWRFFGPDIARAPRPIYLPAIKPPKTVRIFVLGESAAFGDPQP
                     EFGLPRMLEVLLRARWPERRFEVVNAAMTAINSHVLLPIARDCALADGDLWIIYMGNN
                     EVVGPFGGGTVFGPQIPHLALIRASLALNATRTGQLLASLPRLWREHPPDRSEWQGMS
                     MFIEHQLRQDDPRMAR"
BASE COUNT          643 a         1285 c         1138 g          615 t
ORIGIN      
        1 cgatctgcgc cgttttatct tcgagcgatg ggccgatgtg gcgcgtcgct acggattccg
       61 cgagtacgac ggccctccgc tcgaacctct cgaactctac accaccaaga gcggcgcgga
      121 gattgtctcc caactctaca acttcatcga caagggcgga cgcgaggtgg ccatgcgtcc
      181 ggagatgacg ccgaccctcg cccgcatggc gtcggcacag gagcgtcagt acaagaagcc
      241 catcaagtgg tacgccatcc cgcagctttt ccgctacgag cgccagcaga aaggtcgttt
      301 gcgcgagcat ttccagttca acgccgacat tgtgggcgag gcggagccgg ccgccgacgc
      361 cgagctgata gccctgttga tcgattcgtt gcgcgcgttg gggctgacgg cgtccgactt
      421 cgtcgtgcgg ctcagcagcc gcaatgcgtg gcaggacttc ttcagtcgtc attgcagcga
      481 gagctcgagg gcctgcgaat tctaccaggt catcgacaaa ctcgagcggg aaccgcccga
      541 ggtcagcgcc gccaaactcg atgcgctcgg cttcgcactc gacgatgtcc aggccttcat
      601 caccgccggc cggccgactg acgagttgcg gtcgatcctc gataacctcg ccgcgcgcgg
      661 cttgggcgcg tttgtcaagg tcgattacgg agtcatccgc ggactcgatt actacacagg
      721 cccggtcttc gaggccttcg atctgaaggg ggagttccgg gccatcgccg gaggcggtcg
      781 ctacgacaac ctgatcaaga tcatgtccgg cgggcgcgtc gacctgccgg cgctcggttt
      841 cgggatgggc gacgtggtcc tggccgaact cctcaaagcg cgcggtctga cgcccgatgc
      901 taaaccgcgc ctggccgcgt ttgtggtcat cgagaacgag gcgctgcggg cggattccct
      961 cgcactcatc caacggctgc gggacgccaa ctcgagcgtg gactacacat tagtgcccgc
     1021 gcggtccgac aagcagttca agcgcgccct tgaactgggc gcccgcacga ccgtccggct
     1081 cgaacgtacc ccaagcggag acctgatcgc caaggtcaag gacctcgcca cccggatcga
     1141 acgcgccgtc gcacctgacg atgtggccgg caccgtcgca aacgtcgagg attcccaccg
     1201 ttccatgcag tccgcgcact ccgcgcagtc cgatctccgg gctgagcttt gacaaacaga
     1261 acccttacac accatgcgca ccctcatcct cggcatcgac agcgggacac aatcgacgaa
     1321 ggcgctcctc ctcgacgtcg ccaagaagcg cgttgtcggc acgggttccg ccccgcactg
     1381 cctgttgccg ggcctcccgc caggcgccaa agaacagcgt ccggaaacct ggcgtgtcgc
     1441 gacaaccaag gcaatcaagg ccgccatgaa ggccgcgcgg gccaagccgg ccgaggtccg
     1501 agccatcggc gtgagcggcc agcagcacgg cttcgtcccg ctcgatgccc aaggacaggt
     1561 cattcgccct gccaagctct ggtgtgacac gtccaccgcc gaggagtgcg acgcgatcat
     1621 gtccgcgttg ggcggcctcg aagccacgat cggcgcgctg ggcaacgctg tcctgcccgg
     1681 gttcacagcc tcgaaaatcc tctggctcaa acggcacgag ccgcgcaatt tcgcgcgctt
     1741 gtcgaccatc ctgctgccgc acgactacct caacttctgg ctgaccggcc gggcggtcat
     1801 ggaatacggc gacgccagcg ggaccgccct gctcgatgtc cggacgcgtc gatggtgcgc
     1861 gaaagcgctc gaagcgatcg atccggacct ggtgtccaag ctgccccctc tgcatcggag
     1921 cgatgaaccg gccggcacgc ttcaggcctc aacggcgaag gccctgggcc tcaacccagg
     1981 cgtgctcgtc agcgccggcg gtggcgacaa catgatgggc gccatcggca ccggcaatac
     2041 ccggcccggc gtgatcaccg ccagcttcgg cacgagcggc accatctatg cctgcgccca
     2101 gaaccccgtg gtcgatcccc gaggcgagat cgccgcattt tgcgactcga ccaatcgctg
     2161 gctgccgctc ctgtgcacga tgaacgtcac ggtcgcaacg gagatggtcc gaaacgattt
     2221 cggcatgtcc cacgaggcat tcgagaaagc ggccgccgcc gcccccgccg gctccgacgg
     2281 cctgctgctc ctgccttacc tcgaaggcga acgcacaccc aacgtcccct caggcacggg
     2341 cgtcttcttc ggcgtcgccc cccgcacgtt cagcgccccc cacttcgccc gcgcggcgat
     2401 cgagggcgtc accctcggaa tgaactacgg tctgcgccgg ctcacggagc tcggagtcaa
     2461 gccggcccag atgcgcgcca cgggcggcgg ggccaactcg agactctggc gccaggtcat
     2521 ggccgacgtc ttcgatgcgg aagtggtgac gctcaaggtc ggcgaaggcg cggcttacgg
     2581 cgcggctctc caggcgttct ggtgctggcg cctgcagcga ggcgagaagg tctcgatcgc
     2641 cgagatcacc gatgccttcg tcgccctcga tcgcaaaggg atcgctcagc caaacaaggc
     2701 ccgcgtcgcg cattatcgcg aactgcaggc ccttcaggac gaactgtcgc ggagcctccg
     2761 cggcgcgttc gacacgcacc gcgcccttgt cacagttcgg aaacctgtgt cctcgtcccg
     2821 gcattagatt tcagggccag ggtgattcct ctggaagacc agtgcgcaat gcgccgagtc
     2881 agtcgttgaa agcccatcga caccggtcaa ggcgccggca accggcgccc ttatcgaacc
     2941 tacgcacggc agaccgcgca ccatcgagtc ccgagcccgt gctgccgcgc tcgcgtcggc
     3001 ggctgttccg gcttgtcacc gtggttctcg cgccgctgct ggcgcttgcc ctcatcgaag
     3061 gaagcctgcg gttgaccggg tacgggtatc cgaccggttt ttttcgccgt ggcatggccg
     3121 aaggacgagc cgtgctcctc gacaacgaca agttcggatg gaggttcttc ggtcccgaca
     3181 tcgcccgcgc cccgcgtccc atctacctgc ctgcgatcaa gccgcccaag accgtccgca
     3241 tctttgtcct gggcgagtcc gccgcattcg gcgatccgca gccggaattc ggtctgccca
     3301 gaatgctcga agtcctgctg cgcgctcgat ggcccgagag gcggttcgag gtggtcaatg
     3361 ccgccatgac ggccatcaac tcgcacgtgc tcctgcccat cgcccgggac tgcgccctgg
     3421 ccgacggcga tctctggatc atctacatgg gcaacaacga ggtcgtcgga ccgttcggcg
     3481 gcggaactgt cttcggacct caaatcccgc atctcgcact gatccgcgcc agcctggctc
     3541 tcaacgcgac gcggacgggg cagctcctgg caagcctgcc gcggctctgg cgggagcatc
     3601 cgccggatcg atcggagtgg caggggatga gtatgttcat cgagcatcaa ttgcgtcagg
     3661 acgacccccg catggctcga g
//
LOCUS       DAOQRO010000343         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_27959_length_3656_cov_5.283532, whole genome shotgun sequence.
ACCESSION   DAOQRO010000343 DAOQRO010000000
VERSION     DAOQRO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27959_length_3656_cov_5.283532"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKK20_11480"
     CDS             <1..270
                     /locus_tag="PKK20_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV00551.1"
                     /translation="SPRATASKKPGDKPKHVEVVFLKENDRARMVPVKIGISDDAYTE
                     ILEGVAEGQEVISGSYKALRSELDDGKKVRIGTLTPDKDKATETR"
     gene            267..968
                     /locus_tag="PKK20_11485"
     CDS             267..968
                     /locus_tag="PKK20_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV00552.1"
                     /translation="MSLIRLSRVSRRYQMGTETIHALREVSIEVGRAEYVAIMGPSGS
                     GKSTLMNLIGCLDTPSSGTYELNGTNVSDMDDNELAEVRNKEIGFVFQTFNLLPRANA
                     LHNVELPLVYAGIPSEERRQLALEALTNVGLADRVNHKPTEMSGGQRQRVAVARALVN
                     RPSILLADEPTGNLDSKTGAEIMSLFEALADQGHTIIVVTHEEEIARHARRILRIRDG
                     LIASDERADSHALVD"
     gene            952..2181
                     /locus_tag="PKK20_11490"
     CDS             952..2181
                     /locus_tag="PKK20_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV00553.1"
                     /translation="MHWWIELREGLRIAWDATRANKMRAGLTTLGIVIGIVTVTLMGT
                     AIQGLKRGFNQSISALGSDVLYLERYSWFINSHEEWIRQNRRREITWDQVRGLQRQIT
                     SARAVAPTVNTARPVKYKNRSASSVMVIGTTDQALYTGGTSVANGRFLTAGEAEGGRP
                     VCVLGAQTATNLFRGESPVGARVYVGGYPFDVVGVLEPQGEFLGAFSLDNQVIVPIGQ
                     FTSLFTQRPDYAIHIKARDMDSLEDTREEARGIMRKLRRLAPGQDDDFAINQQDQFIK
                     TFDKITGTIATVGIFITGLSLFVGGIGIMNIMFVSVAERTKEIGVRKAIGAKRRAILV
                     QFLLEAAMICLLGGLLGLAIAAPGTLLLRKVMPATISLSIVGLALLVSLLTGVLAGFL
                     PAWRAARMNPVDALRNE"
     gene            2183..3466
                     /locus_tag="PKK20_11495"
     CDS             2183..3466
                     /locus_tag="PKK20_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV00554.1"
                     /translation="MTVLSPRPRHAGRNLIWAEVKESFVMGIGALTTHKLRSSLTVLG
                     VLVGVFSIIVVMTAMRVLQSNIEKELSQLGSHTFQIRKWPAVHFGGPEGFEKYLRRKD
                     ITFAHGKAVMEKATLAQSVGIQCGFWTGEAISRYDKTPPNVSLVGATVGGFPARNWIV
                     GEGRGLIESDIDSAKDVCVLGGGLAKILFPHGSALGEKAKFDGIGYTVVGVLEPKGAS
                     LGGDQDNFAVIPLTTGLNRYGRVWRSLSILVQAQNQAMYEDTVEQVRGILRTARKVKP
                     GVEDDFEVFSNDSLISQFKSFTLTVRIGVAVVSSIALVAAGIGIMNIMLVSVTERTRE
                     IGIRRAIGAKKRNIMTQFIMEAIVLCQVGGVIGVLLGILGGNSAAYFLKLPPVIPLDW
                     VVIGLTICSVVGVLFGSYPAYKAANLDPIESLRYE"
BASE COUNT          670 a         1151 c         1191 g          644 t
ORIGIN      
        1 tcgccgcggg cgaccgcgtc gaagaagccc ggagacaagc ccaaacacgt cgaggtcgtc
       61 tttctgaagg agaacgaccg ggcgcgcatg gttccggtca agattgggat cagcgacgat
      121 gcctataccg agatactcga gggtgtcgcg gaaggccagg aggtcatctc cggcagttac
      181 aaggccctgc gctcggaact ggatgacggc aagaaggtcc gcatcggcac cctcacgccg
      241 gataaggaca aggcgacgga aacgagatga gcttgatccg tctcagccgg gtttcacggc
      301 gttatcagat ggggacggag acgattcacg cgctccgcga ggtctccatc gaggtgggcc
      361 gggccgagta tgtggcgatc atggggccgt cgggctcggg aaaatccacc ctgatgaacc
      421 tgatcgggtg tctcgacacg ccgagttcgg gcacatacga actcaatgga acgaacgtca
      481 gcgacatgga cgacaacgag ttggcggagg tccggaacaa ggagatcggg tttgtgttcc
      541 agacgttcaa cctgttgccg cgcgccaacg cgctgcacaa cgttgagctg ccgctggtgt
      601 acgccggcat tccctccgaa gagcgccggc aactggcgct cgaagccctg acgaacgtcg
      661 gcttggccga cagggtcaat cataagccca cggagatgtc gggaggccag cgccaacgcg
      721 tggccgtggc ccgcgccctg gtcaatcgcc cgtccatcct gctggcggat gagccgaccg
      781 gcaacctcga ctcgaagacg ggcgccgaga tcatgagctt gttcgaggcc ctcgccgatc
      841 agggccacac catcatcgtc gtcacccacg aggaggagat cgcccggcac gcccgccgca
      901 tcctgcggat tcgcgacggt ctgatcgcca gcgacgaaag ggcggattcg catgcactgg
      961 tggattgaac tgcgcgaggg gctgcggatc gcctgggacg ccacgcgggc caacaagatg
     1021 cgggccggcc tgaccacgct gggcatcgtg attggcattg tgaccgtcac gctcatgggg
     1081 accgcgatcc aggggctcaa gcgtgggttc aaccagagca tctcggctct gggcagcgac
     1141 gtcctctatc tcgaacgcta cagttggttc atcaattccc acgaggagtg gattcgccag
     1201 aatcgccggc gcgaaatcac ctgggaccag gtccggggac tccagcggca gatcacatcg
     1261 gcgcgcgccg tcgctcccac cgtgaacacc gcgcgcccgg tgaaatacaa gaaccgcagc
     1321 gcctcgtcgg tcatggtcat cggaacgacg gatcaggccc tgtacaccgg cggaacgagc
     1381 gttgccaacg gacggtttct gacggcgggc gaggcggagg gggggcgtcc cgtgtgcgtg
     1441 ctcggggcgc agacggccac gaacctgttt cggggcgagt cgcctgtcgg ggcgcgggtc
     1501 tatgtggggg ggtacccgtt cgatgtggtg ggtgtgctcg aaccgcaggg ggaattcctg
     1561 ggcgcgttca gtctggacaa ccaggtgatc gtccccatcg ggcaattcac atcgctcttc
     1621 acccagcggc ccgactacgc catccatatc aaggcccgcg acatggacag cctcgaggac
     1681 acgcgcgagg aagcccgcgg tatcatgcgc aagctgcggc gcctggcgcc gggccaggac
     1741 gacgattttg cgatcaacca gcaggaccag ttcatcaaga cgttcgacaa gatcaccggc
     1801 acgatcgcca cggtcggcat attcatcacc ggactctcgc tcttcgtcgg aggcatcggc
     1861 atcatgaaca tcatgttcgt gtccgtggcc gagcggacca aggagatcgg cgtgcgcaag
     1921 gcgatcgggg ccaaacgccg ggcgattctg gtgcagttcc tcctcgaagc ggcgatgatc
     1981 tgtctcttgg gagggctcct gggcctggcc atcgccgcac ccggcacgct gcttctgcgc
     2041 aaagtgatgc ccgcgaccat ttccctgtcc atcgtcggcc tcgccctgct ggtctcgctg
     2101 ctcaccggcg tgctggccgg tttcctgccc gcctggcgcg cggcgcgaat gaacccggtg
     2161 gacgcgttgc gcaacgagtg acatgaccgt tctctctcca cgcccgcgcc acgccggccg
     2221 caacctgatc tgggctgagg tcaaggagag ctttgtcatg gggatcgggg cgctgacgac
     2281 gcacaaatta cgttccagcc tgactgtctt gggcgttctg gtgggtgtgt tctcgatcat
     2341 cgtggtgatg acggccatgc gggtgctgca gagcaacatc gagaaggaac tcagccaatt
     2401 gggcagccac accttccaga ttcgcaaatg gccggccgtg cacttcggcg gaccggaggg
     2461 cttcgaaaag taccttcgcc gcaaggacat caccttcgcc cacggcaagg cggtgatgga
     2521 gaaggccacc ctggcgcaat ccgtcggcat ccaatgcggg ttctggaccg gcgaggcaat
     2581 ctcgcgttac gacaagacgc cgcccaatgt cagcctggtc ggcgccaccg tgggcggttt
     2641 ccccgcgcgc aactggatcg tcggcgaagg ccgcgggctg atcgaatcgg atatcgacag
     2701 cgcgaaggat gtgtgcgtgc tggggggcgg cctggccaag attctcttcc cccacgggtc
     2761 ggctttgggc gagaaggcca aattcgacgg catcggctac acggtggtcg gcgtgctcga
     2821 acccaaaggg gcctcgctcg ggggcgacca ggacaacttc gccgtcatcc cgctcacgac
     2881 gggtctcaac cgctacgggc gtgtgtggcg tagcttgtcc atcctcgtgc aggcccagaa
     2941 ccaggcgatg tacgaggata ccgtcgagca ggtgcgcgga atcctgcgca cggcacgcaa
     3001 ggttaagccg ggagtcgagg atgacttcga ggtgttctcg aacgactccc tgatctcgca
     3061 gttcaagagc ttcactctga ccgtccggat cggcgtcgcg gtggtcagtt cgatcgccct
     3121 ggtggcggcg ggcatcggga tcatgaacat catgctggtg tcggtgaccg agcgcacgcg
     3181 cgagatcggc attcgccgcg cgatcggagc caagaagcgg aacatcatga cccagttcat
     3241 catggaagcc atcgtgctct gccaggttgg cggagtgatc ggagttctct tgggcatcct
     3301 cggcggcaat tcggccgctt acttcctcaa gctcccgccc gtgatccccc tggattgggt
     3361 cgtgatcggg ctcacaatct gttcggtcgt cggcgtcctg ttcggctcct acccggctta
     3421 caaagccgcc aatctcgacc ccatcgagtc gctccgctac gagtgagttc cactgccccg
     3481 ccccgccctc acgcaacttt tccgtggcca gccgcgggtg cgccagcata acctccggcc
     3541 ccatgacgga ccgcaccact cgagtgaccc tgggcgcaaa gctttttctg ccctttgttg
     3601 tcggcgacct cctcctggtg gcggccgccc tggtgatctg gcagcaggcg caccgg
//
LOCUS       DAOQRO010000344         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_28107_length_3645_cov_3.949582, whole genome shotgun sequence.
ACCESSION   DAOQRO010000344 DAOQRO010000000
VERSION     DAOQRO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28107_length_3645_cov_3.949582"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKK20_11500"
     CDS             <1..189
                     /locus_tag="PKK20_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00555.1"
                     /translation="QPLWWSLLLALPAYVWWLTCQQNRLQRLVGVFLDTLGEDLDLTR
                     LPADSDARTSPRRSGDSG"
     gene            515..1501
                     /locus_tag="PKK20_11505"
     CDS             515..1501
                     /locus_tag="PKK20_11505"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNV00556.1"
                     /translation="MKHYLDFEKPIQELQRKLDEMRRHPQMHSLEISLDDEVLQIEKK
                     IAETRRDVFSNLTAWQRVQMARHPKRPYTLDYLGTTFSDFEELHGDRAYSDDRAVVGG
                     FARLQGEPILVVGTQKGRDTKENILRNFGSAHPEGYRKALRLMRLAEKFQLPIVTLID
                     TAGAYPGIGAEERHIAEAIAVNLREMMLLSVPIVAVVLGEGGSGGALGLGVADRVLIL
                     ENAYYSVISPEGCAAILWKDRSAAPKAAEALKITAKDLLELKLVDEIVPEPLGGAHND
                     LPTMAETLRVYLLRHLHEVEAVPPDARLRQRYQKFRSFGHFDEAVPSPPENG"
     gene            1520..>3645
                     /locus_tag="PKK20_11510"
     CDS             1520..>3645
                     /locus_tag="PKK20_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNV00557.1"
                     /translation="MLYPLVFQPILKEHVWGGRKLEELYAKALPPGLQIGESWEISDR
                     PEGVSVVINGPLAGKNLRWLMEHHAAELLGSAGATNGRFPLLVKLLDCREAPSVQVHP
                     PDDVAARSGDEPKTEIWYITRAEPDARLTAGLVRGVKRHQFERKLGEGRVLECLHTIP
                     VQAGDVMFVPGGRVHTIGGGIVLCEIQQNSNTTYRLFDWNRLGPDGKHRELHIQKALA
                     AMDYDDIEPDLVRTDFEGDDAAVTRRLADTPWFQVEEWQSHTPNRRVLNAPNRPIIVG
                     ILSGKATLVGAGETVSLGPGQFCLIPASVRDTAVQTDGKATCLLAVPSGASEVRDPVA
                     SQTSEAPRGIPPWRQHQARGGRMRTHHAAESKARRKASSSRLEIKLILGDRLNDWPLV
                     RLLVLHFWFRMVVIGLVMAFVLGVAVLAKVWPTTPSGFRPKVRVSLLDKVQAYMLRSS
                     AEKAVARGDHEAALLSWRNAFANDMGNPALARGVIRSTTALDPIPTASLGPTVQTSDW
                     LLRLTATNRADLELVCAFYDKAQLYGLANAELEPFSGELTGRLKPIYVKCLFFQGNFD
                     RYNELWAQLSSDEKAQPDMALIHAAYQVGWGRIGSASEVNQLLDKTARERGPLWFLAN
                     RLEMAISVAQSNPDRYRAALDRLQEAQLDNAVDNADYWMLLLGSGRRAEAVRLAQQFV
                     IPPRNQGELVRLATVKYCAATRFRSS"
BASE COUNT          672 a         1085 c         1187 g          701 t
ORIGIN      
        1 cagccgctgt ggtggtctct gcttctggcc ttgcccgcct atgtgtggtg gctcacttgt
       61 cagcaaaacc ggcttcagcg tttggttgga gtctttcttg acacattggg cgaggatctc
      121 gacttgacga ggctgccggc ggacagcgat gccaggacga gtccgcgccg gagcggcgac
      181 tccggctgaa gggggtcaag cgttccgcgg attgggaatt cgacgttgcg gggccatgaa
      241 gaggcgtcta cagtgcgccc catgcaggct ggataggcaa gaccgcgccg ggttcgataa
      301 accgagaaat gcaacatact gttcccatga caggacgcct tcagaaacga cgggaggctt
      361 gtggttgtct ttcttgtggc ggttccgcca cagccctgga ctcggcgttc tcgtcccggc
      421 atgggatgtt cgggccaggg gtgctcttag ccgtggtttg gagaggcatg ctgcttcggc
      481 gaacgaagtg gcttccgagc cgtggttccg tgctatgaaa cactacctgg acttcgagaa
      541 accgatccag gaattgcaga ggaagctgga cgagatgcgg cggcatcccc agatgcattc
      601 cctcgaaatc agcctcgatg acgaggtgct gcagatcgag aagaagatcg ccgagacgcg
      661 gcgtgatgtg ttttcgaacc tgacggcttg gcagcgggtc cagatggccc gccatcccaa
      721 gaggccgtac accctcgatt atctcggcac gacgttctcg gattttgagg aactgcatgg
      781 cgatcgggcc tactcggacg accgggctgt tgtgggtggg ttcgcccggc tgcagggaga
      841 gcccatcctg gtggtaggca ctcagaaggg acgggatacc aaggagaaca ttctgcgcaa
      901 cttcgggtcc gcgcatccgg agggctatcg caaggcgctg cgcctgatgc gcctggccga
      961 gaaattccaa ctgccaatcg tgaccttgat cgacacggcg ggcgcatatc cggggattgg
     1021 ggcggaagaa cggcacatag cggaggcgat cgccgtcaac ttgcgggaga tgatgttgct
     1081 ttcggtgccg attgtcgcgg ttgtgctggg agagggtgga tccggagggg ccctcggcct
     1141 tggggtggcc gatcgggtgt tgatcctcga gaatgcctat tactccgtga tcagtcccga
     1201 aggctgcgct gccattctct ggaaggaccg ttcggctgcg cccaaggcgg ccgaggctct
     1261 gaagatcacg gcgaaagacc tgctcgagct caagctggtc gacgagattg ttcccgagcc
     1321 tttgggcggc gcgcacaacg acctcccgac catggcggag acgctgcggg tctatctctt
     1381 gcgccacttg cacgaggtcg aagccgttcc ccccgacgcg cgcttgcgtc agcgctacca
     1441 gaagttccgc agtttcggtc atttcgacga agccgttccc agcccgcctg agaatggctg
     1501 atccgccaaa agacacgcca tgctgtaccc attggttttt cagcccattc tcaaggagca
     1561 cgtctgggga gggcgcaaac tggaggaact ctatgccaag gcgctgcctc caggactcca
     1621 gatcggcgaa tcgtgggaaa tctcggatcg tcctgagggc gtcagcgttg tcatcaacgg
     1681 acccctcgcc ggcaagaatc tgcgatggct catggaacat cacgccgccg aactgctggg
     1741 atcggcgggg gcgacgaacg ggcggttccc actgctggtc aagttgctcg actgtcggga
     1801 ggcgccctcc gtgcaggtcc acccacccga cgacgtggcg gcccgatcgg gtgacgagcc
     1861 caagacggag atctggtaca tcactcgggc ggagcccgac gcccgtttga ctgcaggcct
     1921 tgtccgcggc gtcaagcgcc accaattcga gcggaaactg ggcgaagggc gggtgctgga
     1981 atgcctccac accatccccg ttcaggccgg cgatgtcatg ttcgttcccg gcgggcgggt
     2041 tcacaccatt ggcggcggga tcgtcctgtg cgagatccaa cagaactcga acaccacata
     2101 ccgccttttc gactggaacc ggctggggcc tgacggcaag catcgagaac ttcacatcca
     2161 aaaggctctg gcagcgatgg actacgacga catcgagccc gacctggtgc ggaccgactt
     2221 cgagggggac gatgcggccg tgacccgccg gctggccgac acaccttggt ttcaagtcga
     2281 ggagtggcag agccatacgc ccaatcggcg ggtcctgaat gcgcccaatc gcccgatcat
     2341 tgtcgggata ctgtcaggca aagccacatt ggtcggtgct ggcgagaccg tgagtctcgg
     2401 cccaggtcag ttctgcctca tccccgcgag tgtgcgtgac actgccgtcc agacagacgg
     2461 caaggcaacg tgcctgctgg ctgttccgtc gggcgcgagc gaagtgagag atcctgtcgc
     2521 gtcccagacg tccgaggctc cccggggcat acctccgtgg cgacagcacc aggccagggg
     2581 cggcagaatg cggacgcatc acgctgccga gtccaaggct cgccgcaagg cgtcgtcgag
     2641 tcgtttggag atcaagctga ttctgggcga cagactcaat gactggcctt tggtcaggct
     2701 tctggtcctg cacttctggt tccgtatggt cgtcatcggc ctggtgatgg cgtttgtgct
     2761 cggcgtggcc gtgctggcca aggtctggcc gacgacaccc tcgggttttc gtcccaaggt
     2821 cagggtgagc cttctggaca aggtgcaggc ctatatgctg cgctcgagcg ctgagaaggc
     2881 cgtggcccgc ggggatcacg aggcggccct gctgagctgg cgaaacgcgt tcgccaacga
     2941 catgggcaac cccgcgttgg cgcgcggtgt gatccgcagc acaacagcct tggacccgat
     3001 tccgactgcg agccttggcc cgacggtcca gacatcggat tggctcttgc gtttgaccgc
     3061 gaccaaccgg gcggacctcg agttggtttg tgccttctat gacaaggcgc agctttacgg
     3121 attggcgaac gccgagctcg agcctttttc cggagaactc actgggcgcc tgaagcccat
     3181 ttacgtcaag tgcctcttct tccagggcaa ttttgaccgc tacaatgaac tgtgggcaca
     3241 gctttcttcc gacgagaagg cccagcccga catggctctg atccacgccg cgtatcaggt
     3301 cgggtggggg aggatcggct cggcgtccga agtcaaccaa ttgctcgaca agaccgcccg
     3361 agaacggggg ccgctctggt ttctggccaa tcgcctcgag atggcgatta gcgtcgccca
     3421 atccaatccg gatcgctatc gcgcggcgct cgatcggctt caagaggcgc aactggacaa
     3481 cgcggtcgac aacgccgact actggatgct cctccttgga agcgggcggc gggccgaggc
     3541 cgttcgactc gcccagcagt ttgtgattcc tccccgcaat cagggggagt tggtgcgatt
     3601 ggcgaccgtc aaatactgtg cggcgacccg cttcagatcg tccga
//
LOCUS       DAOQRO010000345         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_28214_length_3636_cov_3.405753, whole genome shotgun sequence.
ACCESSION   DAOQRO010000345 DAOQRO010000000
VERSION     DAOQRO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28214_length_3636_cov_3.405753"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKK20_11515"
     CDS             complement(<1..1062)
                     /locus_tag="PKK20_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV00558.1"
                     /translation="MSDKPRVSSLSRTVLLGLLGCGVSLTGCGRPQQARPPAGMPEVA
                     VVTVTNRPIVLTTEMPGLTSPYLISDVRPQVNGLVLKRLFIEGSDVKQGQQLYQIDPA
                     PFQAALDNATAALGRAEASLPALRSRVDRFRQALADKAVSQQDFDDAEAMLKQAEADA
                     AYWKASVESARINFEYAKVESPISGRIGKSSVTDGAIVTAYQPIPLATIQQLDPIYVD
                     VTQSATEFLRLQHRLDAGLLSQNTTSFNKVELCLADGTRYAEEGTLQFRDVSVDPTTG
                     SVTLRMVFPNPGSVLLPGMFVRAVVKEGANERAILVPQQAVSRDPRGNPLVMVVNGQG
                     LVEQRQIGLERALDDEWLVS"
     gene            complement(1171..1533)
                     /locus_tag="PKK20_11520"
     CDS             complement(1171..1533)
                     /locus_tag="PKK20_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV00559.1"
                     /translation="MDEKRIKPLIVEVDESTFDSEVFDSAQPVLVAFGAPWSRPCHIL
                     ETTLDEVATACAGSVKLVKVNADDNPDLSICYEVESIPTLLFFLEGQLAAKIVGTVSK
                     EAILARLRAVLDCGEAAS"
     gene            1968..3605
                     /locus_tag="PKK20_11525"
     CDS             1968..3605
                     /locus_tag="PKK20_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV00560.1"
                     /translation="METASDRRFDPNVAAQLLLDIAHEQSVERVLQKFLRRAVERPDI
                     VCMQVWLIGEGDVCSGCPHRLRCSDRSRCWHLVAGQCRPLGDQGVDGLRFDDPSGRIP
                     MGLGLLGSVAATGEDRLVEDMDGEAEGIVGVDLLRRHGVSGCGAVPIIYKSELLGAII
                     GFVRGDMPGHSRSWGRVFANQIGAAIANARAFEEIQRLKAQVELQNTYLQEEVVEAKA
                     FGDLIGRSAVLRQVVSQIDLVAPTDASVLILGETGTGKDLVAHEIHRRSGRRDGPMIR
                     VNCASIPKDLFESEFFGHVQGAFTGAIKDRAGRFETAERGTIFLDEIGEIPLEMQGKL
                     LRVLHEKRYERVGEDRTRHSDVRVIGATNRDLKKAVAAGRFREDLYYRLHVFPIHVAP
                     LRERRDDIPLLAEHFVELSVKELRCQRPRLTRAGVARLEAYDWPGNVRELRNVIERAV
                     ILARGGPLQFDLPVADPTPMLGASKARSRHTNAPGFFTEVEMENLERENLLAVLGKAS
                     WKIKGRNGAAELLGIKPTTLLSRMKKMGVERPNPHIS"
BASE COUNT          677 a         1021 c         1225 g          713 t
ORIGIN      
        1 cgaaacgagc cattcatcgt ccagggcccg ttccagaccg atctgtcgct gttccaccag
       61 gccttggccg ttcacgacca tgaccagcgg gtttcctcga ggatctcgcg atacggcctg
      121 ctgcggcacg aggatcgccc gctcattcgc gccctcctta accaccgcgc ggacgaacat
      181 gccggggaga aggacggacc ccggattcgg aaagaccatt cgcagtgtca cggaaccggt
      241 cgtgggatcg accgaaacgt cgcggaactg cagtgtccct tcctccgcgt acctggttcc
      301 gtcggcaagg cagagttcga ccttgttgaa gctggttgtg ttctggctca gcagacccgc
      361 atccaggcgg tgctgcaagc gaagaaactc ggtcgccgat tgcgtcacgt ccacatagat
      421 cgggtccaac tgctgaatcg tggccagcgg aatcggctgg taagccgtca caatggcgcc
      481 gtccgtgacc gatgacttgc caatccggcc cgagattggg gactcgacct tcgcgtactc
      541 gaagttgatg cgcgcgctct cgacagacgc tttccaatag gccgcgtccg cctcggcctg
      601 tttcagcatc gcctcggcgt catcgaagtc ctgctggctg accgccttgt ccgccagcgc
      661 ctgcctgaag cggtccaccc gcgaccgcag cgccggcagg ctggcttctg cccggccgag
      721 ggccgccgtc gcattgtcga gcgccgcctg gaagggggcc ggatcgattt gatagagttg
      781 ctggccctgc ttgacatcgg agccttcgat aaacaggcgt ttcaagacca gcccgttaac
      841 ctgaggacgg acgtctgaga tcaggtaagg cgaagtgagg ccgggcattt cggtggtcag
      901 aacgatcggc cgattggtca ccgtgacgac ggccacttcg ggcatgccgg cgggcggtcg
      961 ggcctgttgc ggccgaccgc agcctgtgag ggacaccccg caccccaaca gacccagaag
     1021 caccgtccga ctcagagatg agacccgagg tttgtcggac atggtggtcc gggccttccc
     1081 tccgtcttcc gattctttgg ccaaggaatt cgcctctcgc gttgccagtt tactcatttc
     1141 ggggttcatt gtcttcggtt actttcagag tcaggaggcg gcctcaccgc agtccaggac
     1201 ggcccgcaac ctggccagaa tggcctcctt gctgaccgtg cccacgattt tggcggcgag
     1261 ttgcccctcg agaaaaaaaa gaagtgtcgg aatggactcg acctcgtagc agatgctcag
     1321 gtcgggattg tcgtcggcgt tcactttgac caacttcaca ctcccggcgc aggccgttgc
     1381 gacttcatcg agcgtcgttt ccagaatgtg acacgggcgg ctccagggcg ccccgaacgc
     1441 caccaggaca ggctgtgcgg aatcaaacac ctccgaatcg aatgtcgatt cgtcgacctc
     1501 gacaatcagg ggcttgattc gcttctcgtc cattgttctg tgtacgtctc tgcagaaacc
     1561 gtcagttagc atggtttatg ccagtgcgtc attttattcg taaagtgctt gtgcgcaatg
     1621 tgaaacatat cgaacgacat caccacaaga tgcagggacc gtacgaaaac tcgaacgttc
     1681 aaggggcaag agaaggaacg agcgcaggga cgaacgcaga gggacgatgg cgcggctgcg
     1741 ggaacgcccc aggctcgagt caatcacagt cagcgatcaa ggcctcgtgt ggaattcggc
     1801 cctccgaaat gacgtagggc ggcgcatgga gaaccagggc tggcatctga taggccgcaa
     1861 atgccggctg ctctgcgcat gagtcacata ttcgaaacat cgaccgttcg agaattcgtc
     1921 ctttttgatt ggactcgttg tggggggtgg ggcacggttg gccgacaatg gaaactgcga
     1981 gtgaccgccg gttcgatccg aatgttgccg cgcaactgct attggacatt gcgcacgagc
     2041 agtcggtcga gcgagtgctg caaaagtttc tgcgccgcgc ggtggaacgt cccgacattg
     2101 tctgcatgca ggtatggctg attggggagg gggatgtctg ttcggggtgt ccgcatcggc
     2161 tgcgatgttc ggaccggtcg cgctgctggc acctggtggc tggccaatgc aggccgctcg
     2221 gagatcaggg ggtggacggg ctgcgtttcg acgatccgag cggcaggatt ccgatggggc
     2281 tgggtctgtt ggggagcgtg gcggcgacgg gcgaggatcg tctggtcgag gacatggacg
     2341 gcgaggccga gggcatagtc ggcgttgatt tgctgcgtcg gcacggggtc tccggctgtg
     2401 gggcggttcc gatcatctac aagagcgagt tgctgggggc gatcattggg ttcgttcggg
     2461 gtgatatgcc gggtcattcg cggtcttggg gccgggtttt tgcgaaccag atcggggcgg
     2521 cgattgcgaa cgcgcgtgcg ttcgaggaga ttcagcgtct caaggcgcag gtcgaactgc
     2581 agaacaccta cttgcaggag gaagtagtcg aggccaaggc gttcggggac ttgatcgggc
     2641 ggagcgcggt gttgcggcag gtggtgagcc agattgacct ggtggcgccg acggacgcat
     2701 cggtgctcat tctgggtgaa actgggaccg gcaaagacct ggtggcacac gagattcatc
     2761 gccggagcgg acgcagggac gggccgatga tccgtgtcaa ttgcgcgtcg attccgaagg
     2821 atctgttcga gagcgagttc tttggccatg tgcagggcgc gttcacgggg gccatcaagg
     2881 accgcgcggg ccggtttgaa accgcggagc gggggacgat cttcctggac gagatcgggg
     2941 agattccgct cgagatgcag ggcaagctgc tgcgtgtgtt gcacgagaaa cggtatgagc
     3001 gggttgggga ggatcggacg cgccacagcg atgtgcgggt gatcggggcc accaatcgcg
     3061 atttgaagaa ggcggtggcg gcgggccgct ttcgcgagga tctctactat cggctgcacg
     3121 tgtttccgat tcacgtggct cctcttcggg agcgcaggga cgacatcccg ctgctggcag
     3181 agcacttcgt cgagttgtcg gtgaaggaat tgcggtgtca gcggccccgg ctgacgaggg
     3241 cgggcgtcgc ccggcttgaa gcctacgact ggcctggaaa cgtgcgcgag ttgcgcaacg
     3301 tgatcgagcg cgcggtcatc ctcgctcggg gggggccgct tcagttcgac ttgcctgttg
     3361 ccgatccgac cccgatgctc ggcgcatcca aggcgcggtc ccgacacacc aatgctccgg
     3421 ggtttttcac ggaagtcgag atggagaacc tcgagcgcga gaacctcctg gcggttctcg
     3481 gcaaggcgag ttggaagatc aaaggcagga atggcgccgc tgaactgctc ggcatcaagc
     3541 cgacgaccct gctgtcgcgg atgaagaaaa tgggagtgga gcggcctaac ccgcatattt
     3601 cttagcccgt cagtgcacag ggcggtcaga ctacgc
//
LOCUS       DAOQRO010000346         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_28374_length_3623_cov_5.073430, whole genome shotgun sequence.
ACCESSION   DAOQRO010000346 DAOQRO010000000
VERSION     DAOQRO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28374_length_3623_cov_5.073430"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>3623)
                     /locus_tag="PKK20_11530"
     CDS             complement(11..>3623)
                     /locus_tag="PKK20_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00561.1"
                     /translation="SGWQLDGAVRFTFKPGTVLPARAALFVSPDVRAFRARSTSPRGG
                     ERRLVLGPYKGDLSARGETLTLSDNRGRLVHTRTYAGSPSLAQQFLRITEIMYHPAPL
                     AGHPDIEESEFEFIELRNTGRVPLDLRGVRFTDGIQFDFAAGSITTLEAGARVLVVRN
                     ADAFSLRYGTGLPVAGEYTGLLDNSGERLRLEDSYGEKILEFTYDDHWYPITDGHGFS
                     LAIADDTVEWTRWSDRQSWRPNGILHGTPGLLDPPLPTLPPVVINEVLAHSTGPQKDA
                     VELHNPTDAPIDIGGWLLTDTFSWPEKYRLPSPTIIPALGFVVFTETDFNPTPGQPPS
                     FALGANGDDVWIFSADDAGRLTGYVQGFDFEATASGVSLGRHTNSVGDVDYPPLAWVT
                     LGEPNAVPRAGPVVISEIMCHPAATGTTNQPASFIELANISTTNVPLADPAMPDSPWR
                     LRNGVQFAFPPGAALPSGHRLLVVGFDPVADAKARASFISLYAVPPDVPLHGPWQGRL
                     ANDEGTIELVQPAVEQGQETAFMLVDKVHYMDRAPWPTGADGSGASLQRRTLASYGNE
                     PTNWFASTPTAGAVNEPNALPTVQLTAPSQNAVLHWPADLLLSASAQDSDGRIVRVEF
                     FNHGQKLAQITEAPYTFVWTNPPPGAHLLRAVAVDDRLGATTSDATAVTVIAPPPVVR
                     MVAPADGAVLLAGSTIAVAAEAEPAAGQIDRVRLYAGPTVLAELLAPPFEFTWPAVPA
                     GVYDLQAVALDSFGLASTSAVARVGFSTGTIAASTIVPTGSRWKYLDDGSAPDRAWTT
                     LEFDDSGWKSGVAELGYGDAREGRPEATQIGFGPDAANKHITSYFRHLFTVTNVFAYR
                     DLTVGLMRDDGAAVYLNGIEVFRSNLPEGPIHSRTLASYAVSGAEESAIFTQSVTPAR
                     LREGPNMLAVEVHQASPGSTDLSFDLALSGTRVFLAPLLLAQPQSQSVARGATATFTA
                     WAGGTEPLAFQWRFNHVPILAATNSTLTLSDVQPSHAGAYQLVVRNALGAAVSEPATL
                     TLTNRPPTTVDDGILVPQGQTAFVSASLLTSNDSDPDGDAVRLISVSPLSEQGASVAV
                     VSGQVRYQPPAAFQGADRFTYAVADPQGLASTGNVAVFVYAGPLPTLNRLTVVPESTV
                     LRIRYSGGPGRTFEWQRSLDLRHWEVLTSGVIPPHSFVEWIEPLSTAGRAYYRVVQR"
BASE COUNT          646 a         1101 c         1266 g          610 t
ORIGIN      
        1 ggtgttgacc tcatctctgc acgacgcggt aataggctcg gccggcggtc gacaacggct
       61 cgatccactc gacgaagctg tgaggcggaa tgacgcccga ggtcagaacc tcccaatgcc
      121 tcaggtcgag actccgttgc cattcgaacg tccgccccgg tccgcccgag tagcgaatac
      181 gaaggactgt cgattccgga acgacggtca gtcgattcag agtcggcagc gggcccgcat
      241 acacgaacac ggcgacgttg ccggtcgagg cgagaccctg cggatcggcg acggcatagg
      301 tgaaacgatc ggcgccctga aaagcggctg ggggctgata gcgcacctgg cccgagacga
      361 ctgcgacgct ggcgccttgt tcgctcaagg gactcacgct gatcaagcgc accgcgtcgc
      421 cgtcgggatc cgagtcgttg gatgtcagga gcgaggcact cacgaaggct gtctggccct
      481 ggggcaccag aatcccgtca tccacggtcg tcggcggacg attcgtgagc gtcagcgtgg
      541 caggttcgct cacggcggcg cccagggcgt ttctcacgac cagttggtag gcgcccgcgt
      601 ggctcggttg gacgtcgctg agcgtgagcg tcgagttggt cgccgcgaga atcgggacat
      661 ggttgaatcg ccactggaat gcgagcggtt cggttccgcc ggcccaagcc gtgaatgtcg
      721 cggtcgcgcc acgcgccacc gactgcgact gcggttgggc caacagcagg ggagcgagaa
      781 agacgcgcgt gcccgaaagg gccagatcga agctcagatc ggtgctgccg ggtgaggcct
      841 gatgcacttc aaccgcgagc atattcggac cctcgcgcag gcgcgcaggc gtgactgact
      901 gggtgaagat cgcgctctcc tcggctccgg acacggcgta gctggcgagg gtgcggctgt
      961 gaattggccc ctccggaagg ttacttcgaa acacctcgat tccgttcagg tacacggcgg
     1021 cgccgtcgtc acgcatcaac ccgacggtga ggtctctgta cgcgaacacg tttgtgacag
     1081 tgaacaaatg ccggaaatac gatgtgatat gtttgttggc cgcatcaggc ccgaacccga
     1141 tctgtgtcgc ttccggccgg ccctcgcggg catcgccgta cccgagttcg gcgacccctg
     1201 atttccagcc tgaatcgtcg aactcaagag tcgtccaagc ccggtcgggc gccgagccgt
     1261 catcgaggta cttccatcgc gagccagtcg gcacgatggt ggatgccgcg atcgtgccgg
     1321 tcgagaaccc aacgcgcgca acagcggagg tgctggccag gccgaaggag tccagggcga
     1381 cggcctggag atcgtagacg cctgccggga cggcgggcca ggtgaactcg aacggcggcg
     1441 cgagcagttc ggcgagaacg gttggtccgg catagaggcg cacgcggtcg atctggccgg
     1501 cagccggctc ggcttccgcg gccaccgcga tcgtgctgcc cgcgagcaac acggcgccgt
     1561 cggcgggcgc aaccatacgc acaacaggcg gaggcgcaat cacggtgact gccgtcgcgt
     1621 ccgacgttgt cgcgcccagg cgatcgtcga ctgccacggc tcgcaacagg tgcgcgcccg
     1681 gcggcggatt cgtccatacg aatgtgtatg gcgcctccgt gatttgggcg agcttttggc
     1741 cgtgattgaa gaactcgacg cggacgattc ggccgtcgct gtcttgggcc gatgcgctca
     1801 ggagcagatc ggcgggccag tgcaacacgg cgttctggct gggtgcggtg agttgcacgg
     1861 tgggcaacgc gttgggctca ttcaccgcgc cagccgtggg tgtcgaggca aaccaattgg
     1921 tcggttcgtt cccgtaggag gcgagcgttc ttcgctgcag cgaggcgccc gacccgtcgg
     1981 ccccggtcgg ccacggagcc cggtccatgt agtgcacctt gtccacaagc atgaaagctg
     2041 tctcctggcc ctgctcaacg gccggttgca ccagttcgat cgtgccctcg tcgttggcga
     2101 gtcgcccctg ccaaggcccg tgcagtggca catcaggcgg aacggcgtag agcgaaatga
     2161 aagaggcgcg cgctttggcg tccgcaacgg gatcaaagcc gaccaccagc aggcggtggc
     2221 ccgaaggcag cgcggcgccc ggcggaaacg cgaactggac accgttgcgc agccgccatg
     2281 gggagtccgg catcgcgggg tcagccagtg gaacgttggt ggttgagatg ttggccaact
     2341 cgatgaaact ggccggctga tttgtggttc cggtggctgc cgggtggcac atgatctcgg
     2401 agatgacaac cgggcccgca cgcggcacgg cattcggttc gcccagggtg acccaagcca
     2461 acggcgggta atccacatcg cccacgctgt tcgtgtgccg cccgaggctg acgccactgg
     2521 cggtcgcctc gaaatcgaac ccctgcacgt agccggtcaa acgccccgca tcgtccgcac
     2581 tgaagatcca aacgtcgtcg ccgttcgcgc ccaaggcgaa actggggggc tgccccggcg
     2641 tcgggttgaa gtcggtttcg gtgaagacga caaagccgag ggcgggaatg atggtcggcg
     2701 aaggcaaacg gtatttctcc ggccaggaaa aagtgtcggt gagcagccat ccgccgatgt
     2761 cgatcggggc gtcggttgga ttgtggagtt cgacggcatc cttctggggg ccggtcgagt
     2821 gggcgaggac ttcgttgatg acaacaggcg gcagggtggg cagcggagga tcgagcagcc
     2881 ccggggtgcc gtggaggatt ccgttggggc gccaactctg ccggtcactc catcgagtcc
     2941 actcgacagt gtcatccgcg atggcgaggg agaagccgtg cccatccgtg atcggatacc
     3001 agtggtcgtc gtaggtgaat tcgaggattt tttctccata actgtcctcg agccggaggc
     3061 gctcaccgct gttgtccagc agcccggtgt attcgccggc caccgggagg ccggtgccat
     3121 accgcagcga gaacgcgtcg gcattgcgca ccaccagaac ccgggcgccg gcttcgaggg
     3181 tggtgatcga gccagcagcg aagtcgaatt gaatcccatc ggtgaatcgc acgccacgca
     3241 gatcgagagg gacgcgtcct gtgttgcgca gttcgatgaa ctcgaattcg gattcttcga
     3301 tgtccggatg cccggccaac ggcgccggat ggtacatgat ttcggtgatc cggaggaact
     3361 gctgggccag gctcggagag cccgcatagg tccgcgtgtg gaccagtcgc ccgcgattgt
     3421 cgctcagagt gagcgtttct ccccgggctg ataggtcgcc tttgtaaggg cccaggacga
     3481 gccgtcgttc gccgcctctg gggctggtcg agcgggcgcg gaaggcccgg acgtcgggcg
     3541 agacgaacag cgccgcgcgc gcgggaagaa ccgtcccggg tttgaaggtg aaacggacgg
     3601 cgccgtcgag ttgccagccg gag
//
LOCUS       DAOQRO010000347         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_28552_length_3611_cov_3.556805, whole genome shotgun sequence.
ACCESSION   DAOQRO010000347 DAOQRO010000000
VERSION     DAOQRO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28552_length_3611_cov_3.556805"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PKK20_11535"
     CDS             <1..1067
                     /locus_tag="PKK20_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV00562.1"
                     /translation="FNAQQLVALVRSFAPGDKVTLALVRQGDRQTLDVTLGETEIAPA
                     GKSLSDVWDGARDATGIGRLFGLPLDKAIIPNLTIQPPDKPSAYLGVELGYVISPLAT
                     QLGLEDESGALVNMVVDESPAARAGLKRHDIIVKLDGKKVRDPADLSARIDKHKKGDK
                     ITLTVIRGGKPIEITATLSEKAATDPARLQKLLREYYPIVPGIRMLRTGPDRNEIVVR
                     LDSENDGNAYHVERRIVVDPANARATAESAIVKPGHSTTSQVSVEQKVIVMKTDGGMS
                     TVLEEKDGHRHVTVKDAEGKVVFDGPVNSDGDRQRLPTEAREQLDRIEQSLKAGLDSV
                     GKDVIHELRLAPGPTVPDVI"
     gene            1145..1783
                     /locus_tag="PKK20_11540"
     CDS             1145..1783
                     /locus_tag="PKK20_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV00563.1"
                     /translation="MKTLTLCVWVVLALGFPFNEQGQAAGLVWHSSRAETVTAARNTA
                     KLILLLAGRDACGNCMYMKTTVCESPEVRALIDGNYVCWYCQIDYSTEWQAYRGGLGG
                     FTLPLICAIDPGDSLNYLDRSTSIQTISVFQPRLRSHLPTSEIRASPTFESGAPRLLW
                     HGESGFRYRVLESADFAAWSLVGALVRGDGSPIEFTDVSADTPRFYRIMGFK"
     gene            complement(2037..>3611)
                     /locus_tag="PKK20_11545"
     CDS             complement(2037..>3611)
                     /locus_tag="PKK20_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNV00564.1"
                     /translation="AAMYGLLSTTDMMWFFFIFWQLMTLPGYALIRYEHKQSQNIRAA
                     NKYLVMMQIACAATMVGAWFLAVMGALTSGGASLKYDFDTVSANLPAMLQAKPFDTGV
                     AFLLFLLGFGIKMGMWPFGQVWLPDAHPAAPSPVSAMLSGVMIKTGVYGLMRYFLWLV
                     PPEAHAQYPLARWGWVVAILGTITLFTGTMQALKQEQSKRLLAFHSIGQIGYILLGTG
                     ACMALLPASDPRAAGLAAIAFLGALFHVINHGLFKGLLFLNAGSLRHVTGTQDLNQMG
                     GLMKFMPLTAATALVASLAISGVPLLNGFASKWTIYVATIQGSVAAKYLPVCGVLAIL
                     TSALTLASFMKFFGVSFLGRASALVKARVAAQRKLEVGGLMQGPQVFLALLCVALGVV
                     PALAFHLMTAALEAAQQGFGTALAAAAPMTIGALSGIETVNRTALFAPVMLACVLGVM
                     LLLVRILSKLGGAKRRAAAPWLCGYATESEANRYAAHGFYGEVKRHFHWLGGAPRPTD
                     GDRPSASTTPARRPGH"
BASE COUNT          727 a         1098 c         1165 g          621 t
ORIGIN      
        1 tgtttaatgc gcaacaactg gtagcgttgg tgcgcagctt tgcgcccggg gacaaggtga
       61 ctctcgcgtt ggtccgccaa ggcgaccgcc agacgctcga tgttacactg ggcgagaccg
      121 agatcgcccc ggccggcaaa tccttgtccg atgtctggga tggagccaga gacgcgaccg
      181 gcatcgggag attgttcggg ctccccctcg acaaggccat cattcccaac ctgaccatcc
      241 aacctccgga caagccgtca gcctatttgg gagtggaact tggttatgtc atttccccac
      301 tggcgactca actcggtctg gaggacgagt ccggcgcgct ggtgaacatg gtggttgatg
      361 agagtcccgc cgccagagcc gggctcaaac ggcacgacat cattgtcaag ctcgatggca
      421 agaaagttcg ggatccagcc gacctcagcg ctcgcatcga caaacacaag aagggagaca
      481 aaatcactct caccgtcatt cgcgggggca agccgattga aatcacggcg actctttccg
      541 agaaggcggc caccgatccg gccaggctcc agaaactcct gcgggaatac tacccgattg
      601 tgcctggcat ccggatgctg cgcaccgggc cggatcggaa cgagatcgtg gtccgcctgg
      661 attccgagaa cgatgggaac gcatatcacg tcgagcgccg tatcgttgtt gaccccgcaa
      721 atgccagggc gaccgccgag agcgccatcg tcaagccagg gcactcgacg accagccagg
      781 tttctgtcga gcagaaggtg atcgtgatga agaccgacgg ggggatgagc acggttctcg
      841 aggagaagga cgggcatcgc catgtcaccg tcaaagacgc ggagggaaag gtcgtattcg
      901 acggtcctgt gaactcggac ggagatcggc agagactgcc gaccgaggcg cgcgagcaac
      961 tggatcgaat cgagcagagc ctcaaagcgg gattggacag cgtgggcaag gacgtgatac
     1021 acgaattgcg gcttgcgccc ggtccgaccg ttccggacgt catttagggc gatccgatcg
     1081 ggggaattcc gtcacgcggc cactgtgtct gggtcggagc gggcgcattc gacgaaacac
     1141 atcgatgaaa acgcttacgc tttgcgtctg ggtggtgctc gccctcggct ttcccttcaa
     1201 cgaacaaggc caggcggccg gtctggtctg gcattcgtcg cgtgccgaaa cggtgactgc
     1261 agcccgcaat acggccaagc ttatcctgtt gctcgcgggg cgagacgcat gcggcaactg
     1321 catgtacatg aagaccaccg tgtgcgaatc gcccgaggtg cgcgcgctca tcgacggcaa
     1381 ctacgtctgc tggtattgcc agatcgatta ctcgaccgaa tggcaggcct acaggggcgg
     1441 acttgggggg ttcacgttgc ctctgatctg cgcgattgat cctggagatt ccttgaacta
     1501 tctggaccga tccacctcga ttcagacgat ctccgtattc caacctcggt tgcgcagcca
     1561 tctgcccacc agcgagatca gagccagccc aaccttcgag tccggagcgc cgcgcctgct
     1621 ctggcatggc gagagtggat tccggtatcg agtgctcgag tcggcggatt ttgcggcctg
     1681 gagtcttgtc ggcgcgcttg ttcgcggaga cggttcgccc atcgagttca cagacgtctc
     1741 ggccgacacg ccgcgcttct atcggatcat gggtttcaag tagcgccagc ccggacattt
     1801 ctcagcgcgg caaagccaca accaaattgg gccatacctg acagccaagg cctattcatg
     1861 aagtggagcg gcgtcgccga catgcgccgt cttcacttga catggcgggg gacgaggcgt
     1921 ccccactccg accttctcga cttcatgagt tggcgctgac tggcagcctg tgcggcatga
     1981 gacttctttc aaaaagaaac gaagttgaca gagagtggga caaggctgcg cgcgatctaa
     2041 tgtccgggcc ggcgggcagg cgtcgtcgag gcggacggtc ggtcgccatc ggtcggtcgc
     2101 ggggcgccgc ccagccagtg gaagtggcgt ttgacctcgc cgtagaaacc gtgtgcagcg
     2161 tagcggttgg cttcggattc cgtcgcgtag ccgcagagcc agggagccgc ggcacggcgt
     2221 ttagcccctc cgagtttcga gaggatgcgg accagcagga gcatcacgcc caacacgcac
     2281 gccagcatca cgggggcgaa gagggccgtg cgattcaccg tctcgatgcc gctcaacgcc
     2341 ccgatcgtca ttggggccgc cgcggcgagc gctgtcccga agccttgctg cgcggcttcg
     2401 agcgcggcgg tcatgagatg gaaggcgagc gccggcacga cgcccagcgc cacgcacagc
     2461 agcgccagga acacctgcgg cccctgcatc agcccgccca cctcgagttt gcgctgcgcg
     2521 gcaacgcggg ccttgaccag ggcgctggcc cggccgagaa aactgacgcc gaagaacttc
     2581 atgaacgagg cgagcgtgag cgcgctggtg agaatggcca ggacaccgca caccgggagg
     2641 tacttggccg cgactgaacc ctggatggtc gccacgtaga tggtccattt gctggcgaaa
     2701 ccgttgagga gcggcacacc cgaaatcgcc agggaggcca ccagcgccgt cgccgccgtc
     2761 aggggcatga atttcatcag acctcccatc tggttgaggt cctgggtgcc ggtcacgtga
     2821 cgcagggacc cggcgttgag aaacagcaaa cctttgaaca ggccgtgatt gatcacatgg
     2881 aagagcgccc cgaggaacgc gatcgctgcg agtcccgcgg ctcggggatc ggatgcgggc
     2941 agcagggcca tgcacgcgcc ggttccgagg aggatatagc cgatctgacc gatgctgtgg
     3001 aaggcgagca accgcttcga ttgctcctgt ttcagggcct gcatcgtgcc ggtgaacagc
     3061 gtgatcgtgc ccaggatggc caccacccag ccccagcgcg ccagcgggta ttgggcgtgc
     3121 gcctcgggcg gcacaagcca gagaaaatag cgcatcaggc cgtacacgcc ggtcttgatc
     3181 atcactccgg aaagcatcgc gctcaccggc gagggcgccg ccggatgcgc gtctggcagc
     3241 cagacctggc cgaacggcca catgcccatc ttaatgccga agcccaggag gaacagaagg
     3301 aatgccacgc ccgtgtcgaa cggcttggcc tggagcatcg cgggcaggtt cgcgctgacg
     3361 gtatcgaagt cgtacttgag gcttgcgccg ccgctggtca acgcgcccat caccgcgagg
     3421 aaccatgcgc ctaccatcgt cgcggcgcag gcgatctgca tcatgacgag gtatttgttg
     3481 gccgcccgga tgttctgcga ctgcttgtgt tcgtagcgga tgagcgcgta gccgggcagg
     3541 gtcatcaact gccagaaaat gaagaaaaac cacatcatgt ccgtggtgct cagcaggccg
     3601 tacatcgccg c
//
LOCUS       DAOQRO010000348         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_28815_length_3590_cov_5.560679, whole genome shotgun sequence.
ACCESSION   DAOQRO010000348 DAOQRO010000000
VERSION     DAOQRO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28815_length_3590_cov_5.560679"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..3230
                     /locus_tag="PKK20_11550"
     CDS             168..3230
                     /locus_tag="PKK20_11550"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00565.1"
                     /translation="MKSTARRVDCINTTLERATPEPRSLPGASLRRVGALASECEVGA
                     VARQEGAIMSQLDFNGELRYLAQLTAAGLKPLSRWEGPAGSHIHAALERCGLCWRTIH
                     RTTRSGHPVAELVFSTESRCLDLYSARFEGRSLDRTLNDRRFEGWLFGYPSCCIESYL
                     AHGYRPNALEREDQRLLFHWACPRCQVTPILLPDYRRLHRACLTGERNGLRPGKPFVR
                     SSPRIRRVLRQSLAMAASFVALGAFSPAAWAGASPNPHQIPLDVWTDADRDGLDDAQE
                     LLLGLDPNRADQDNNGISDGVDWALKLSAAIDALPGEVSPTQPYRLAHMAFGLETCSI
                     CGEAVNMGFYEIVNPLENLSAAVPCIAKHYLEHGSLVYAGSEHEGVLNVPLLRTALES
                     AGQAHLLLEGAAVDRDRDGLRDDEERLFNTSPDNPDSDADGLLDGIETARKLRAQLDA
                     LPQFGRPEDGPGDRPFAVPHPMYGIETCPRCGQAVVMDIWHVYNLGNQGFIEVPSMAL
                     HYLKHGAFAWEGGQLAGGKGRVDPRQLLSVLTGQGDGHQSPVSPDQDEDLLTDREELD
                     LGTDPANGDENANHLPDGADLAAACAARIAGLPVKPSADHPYRLDFELKGLERCAVCG
                     TNVNMGHLTVCNARAGLYATLPYIALHHLEHGSFSHAGSVHGSGRLDPVLLRAALDET
                     APSHFVRAREDGDEDGLTGAEEKRLGTDPAVADTDGDGVPDGFAVARRLWESIVHLTR
                     ATTAPVHAVDHLMRGLVTCPVCGKQENMGYIEIVNVPEQMRVEVSYLALHFLRHGSLG
                     NGDGGRLNPRLIHLALEGDGGSHAVIVAGDSDRDGLLDSEERALGTDPERPDTNGDGI
                     VDGAEAARSAFRRIGELPREATAREPYALHAEADCYVPCEICGVDVNCGFVELVQPWA
                     ELRITLSYRLLHFMEHGSFAASLEERADPLQLEAFLRPSLLMVGDGQRPTLRWYGALG
                     RRYQVQTGDTPSGPWRSGPEFYGANAPLTFTVDSTDPAPRRFFRLLVW"
     gene            complement(3314..>3590)
                     /locus_tag="PKK20_11555"
     CDS             complement(3314..>3590)
                     /locus_tag="PKK20_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV00566.1"
                     /translation="PEIVGGAKNFLVENTTVTVTFVQEKAVQIELPAAVVLTVADAPE
                     GIRGDSASNVQKTVTLETGITVQVPLFIKTGEKLRIDTRTGKYIERA"
BASE COUNT          619 a         1180 c         1141 g          650 t
ORIGIN      
        1 tttgacggag cgagatggcg cggactcacg tccgcggcta cggaaccacg gttcaaggaa
       61 agccacctgt tccgttttga actgctcagt gggaatgaac ctggcaccgc aggcgtcccc
      121 cctccaaagt tcggttcatg gaactgccgg tgtccgggct tgcccccttg aaaagcacag
      181 cgcggcgagt agactgcatc aacacaaccc tggagcgcgc gacgcctgaa ccacgcagcc
      241 tccccggcgc gtcgctgaga cgcgtcggcg ccctggcgtc ggagtgcgaa gtgggcgccg
      301 tggcgcggca ggagggagct attatgtcac agcttgattt caatggcgag ctgcgctacc
      361 tggcgcagct cacggccgcc ggcctcaagc ctctgagccg ctgggagggc ccagccggct
      421 cgcacatcca cgccgccctc gaacggtgcg gcctgtgctg gcgcacgatc catcgcacaa
      481 cccgttccgg acacccggtt gcagagcttg tgttcagcac cgagtcgcgt tgtctcgacc
      541 tctattccgc ccgttttgaa ggccggtctc tcgatcgcac tctgaacgat cgccgcttcg
      601 aaggatggct gttcggctat ccctcgtgtt gcatcgagag ttaccttgcc cacgggtacc
      661 gccccaacgc gctcgaacgc gaggaccagc gtctcctgtt ccattgggcg tgtccgcgat
      721 gtcaggtgac acccatcctg ctgcctgatt accggcgcct gcaccgcgcc tgtctcaccg
      781 gcgagcgcaa cggcctgcgc cccggcaaac cgttcgtccg atccagtccg agaatccgtc
      841 gcgtcttgcg ccagagcctg gccatggcgg catcgttcgt cgctctcggc gcgttctccc
      901 ccgcggcatg ggccggagcc tcgccgaatc cgcaccaaat cccgctcgat gtgtggacgg
      961 atgccgatcg cgacggtctc gatgacgctc aggagttgct gctggggctg gatccgaatc
     1021 gggcggatca ggacaacaac ggcatatcgg acggcgtgga ttgggcgttg aaactgtccg
     1081 cggcgatcga cgcgcttccg ggagaggtgt ctccgaccca accgtatcgt ctcgcgcata
     1141 tggcgttcgg gttggagacg tgcagcatct gtggggaagc cgtgaacatg gggttctacg
     1201 aaatcgtcaa tccgctcgag aatctcagcg ccgcggttcc ctgcattgcc aagcactacc
     1261 tcgaacacgg cagtctggtc tatgcgggca gcgagcacga gggcgtcttg aacgtgcctc
     1321 tgctgcgaac ggcgctcgag agcgccggcc aggcgcacct cctgctcgaa ggggccgcgg
     1381 tggacagaga ccgcgatggg ctgcgcgacg acgaggagcg cctcttcaac acgtcgccgg
     1441 acaacccgga cagcgacgcc gacgggttgc tcgacggcat cgaaaccgcg cgcaagctgc
     1501 gggcgcaact cgacgccctc ccccagttcg gacgtcccga agacggcccc ggcgaccgcc
     1561 cgtttgcggt tccgcacccc atgtatggca tcgagacctg tccccgctgc ggccaggcgg
     1621 tggtcatgga catctggcac gtttacaacc tcggcaatca gggattcatc gaggtgccgt
     1681 ccatggcgct ccactacctc aagcacggcg cgttcgcctg ggaagggggc caactggcgg
     1741 gcggcaaggg ccgggtcgat ccgcgccagc ttctgtcggt cctgaccggg cagggagacg
     1801 ggcatcaatc gcctgtttcg cccgatcaag acgaggatct cctcaccgac cgggaagaac
     1861 tcgatctcgg gaccgatcct gccaatggcg atgagaacgc caatcacctg cccgacggcg
     1921 ccgacctcgc cgcagcatgc gccgcgcgca ttgccgggct tcccgtgaaa ccctcggccg
     1981 accaccccta tcgcttggat ttcgaactca aaggactgga acgatgcgcg gtgtgcggga
     2041 ccaatgtgaa catggggcat ctgaccgtgt gcaatgcccg cgccggcctc tacgccaccc
     2101 tcccatacat cgcgctccat catttggagc atggcagctt ttcgcatgca ggcagcgtcc
     2161 acggctccgg tcgcctggac ccggtgcttc tccgcgccgc gctcgatgaa accgcaccca
     2221 gccacttcgt tagggcgcgc gaggatggag acgaagacgg gctcacgggc gccgaggaaa
     2281 aacgcctcgg cacagacccc gcggtggcag acacggacgg cgacggtgtt cccgacggat
     2341 ttgccgtggc gcggcgcctc tgggaatcga ttgtccattt gacccgcgcc acgaccgcgc
     2401 ccgtgcatgc cgtggatcac ctgatgcgcg gcctggtcac gtgtcccgtt tgcggaaaac
     2461 aggagaacat gggttacatc gagatcgtca acgtgcccga gcagatgcgg gtcgaagtct
     2521 cctacctcgc cctccacttt ctccgccacg ggagcctggg caacggcgat ggcgggcggc
     2581 ttaacccgcg cctgatccat cttgcgctcg aaggggatgg cggatcgcat gcggtgatcg
     2641 ttgccggcga cagcgaccgg gacgggctgc tcgattccga ggaacgggct ttgggaacag
     2701 atccggaacg cccggacaca aacggcgacg gcatcgtgga cggggcggaa gccgcgcgca
     2761 gcgcgttccg acgcatcggg gaactgcctc gcgaggcaac agcccgcgag ccgtacgcac
     2821 tgcatgccga ggccgattgt tatgttccgt gcgagatttg cggagtggat gtgaattgcg
     2881 ggttcgtcga actggttcag ccctgggccg agttaaggat caccctctcc tatcgcctcc
     2941 tccattttat ggaacacggc agcttcgccg cctcgctcga ggagcgagcc gatccgctgc
     3001 agctcgaagc tttcctgcgc ccctcgctgc tgatggtcgg cgacggccag cggccgacgc
     3061 tgcgctggta tggggcgttg gggcggcgtt accaggtgca gaccggggac acaccgtccg
     3121 gaccgtggcg gtcaggcccc gagttctatg gagccaatgc gccgttgacg ttcaccgtcg
     3181 actccaccga tcccgcgccg aggcgcttct tccgtttgtt ggtttggtag cccggttgcg
     3241 attcctcgcg aagcgtgtga aataaccggc taagggtctg cgcaaaaaac cggaagtaat
     3301 ttttgcacag accctaagcg cgttcgatgt atttgccggt gcgggtgtcg atccggagtt
     3361 tctcgcccgt cttgataaaa agcggcacct ggacggtgat ccccgtttcg agcgtgaccg
     3421 tcttctgcac gttgctggcg gagtcgcctc gaatgccctc gggggcgtct gccacggtca
     3481 gaaccacggc tgcaggcagt tcgatctgca ctgccttttc ctggacgaaa gtcaccgtga
     3541 ccgtcgtgtt ctccaccagg aagttcttgg cgccgccgac gatttcaggc
//
LOCUS       DAOQRO010000349         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_28960_length_3577_cov_3.776547, whole genome shotgun sequence.
ACCESSION   DAOQRO010000349 DAOQRO010000000
VERSION     DAOQRO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28960_length_3577_cov_3.776547"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..788)
                     /locus_tag="PKK20_11560"
     CDS             complement(168..788)
                     /locus_tag="PKK20_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00567.1"
                     /translation="MTLTALANLLNRDSQDIIGKADYWKTFQCYLRSHRFRQLPPQPA
                     EPGPTRALSDIDAAKPSALNDGEVRLDCIETRQPWIDENLNPNTGDWIARTLLKQRRQ
                     PPDERGKDYNHSTFCDLVITGLVGLRPRPDDIIEVNPLVSDGTWDYFCLDGVRYHGRM
                     LTILYDRTGTRYDRGVGLQVLVDGQSVARSDSLKRITAPAAHAGSP"
     gene            complement(785..1960)
                     /locus_tag="PKK20_11565"
     CDS             complement(785..1960)
                     /locus_tag="PKK20_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00568.1"
                     /translation="MNTAPAPGPTPLGTPGIEPPTNRPDRRRPLLPAWCRLAFIALVL
                     AQIHGAEPFAQASPDPTPGQPVLKADAFQHYVESFNRNDRETVVNHIRNAAAWQSMSQ
                     NIPRFECADKNLEEVYYFRWWTYRKHIKKTPDGFIITEFLPPVPWAGKYNSINCPAGH
                     HFYEGRWLLEPQYLDDYALFWFRKGGEPRKYSFWAADSIHARSLVLGDDRIPQELLPD
                     LIANYEAWEKSHLDETGLFWQNDGNDGMEVSIGGSSCRATINSYQYGDAVAISRIAER
                     AHQHDLAQTWRAKAGALRHLVLTKLWDPEARFFKVLPRGKTTLADVRGLHGYTPWYFD
                     LPEPAMSVAWRELMDSNGFYTLRSVPQLPNNDTRLSQSPTQGTNASGTARVGLWQPL"
     gene            complement(2198..2983)
                     /locus_tag="PKK20_11570"
     CDS             complement(2198..2983)
                     /locus_tag="PKK20_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00569.1"
                     /translation="MLCADSTVIASESLSVDIALEAGTRAPVLQVNGASGLSCRLEYA
                     DVLAGSAPWQLLTNLTLSATGAVAVDRTTTGRTRFCRATHPTDETALLLWLRFNGTLD
                     GTDGERPLSHRGASYRPGQAGQAVYLDATGHIRYPISQNLESDEGTIEFWVQPDWDGT
                     DTEVRVFLEAGDNFNRGLLISKDGASNLRFPQWGDDPGTPAVESDVERGLGFDGSHWR
                     KGQWYHLAVAWRGTTRELAFYENGEVIRTAVNGVHLPSFGTSS"
BASE COUNT          620 a         1036 c         1228 g          693 t
ORIGIN      
        1 cgtgcagcat tacgatgccc tcctgagcgc cgtggctgcc gcccgtgtcg aggccgctgc
       61 cgggcgcgga ttgcccgctc cggacaggat cgggctggcc ttgccttagg gtctgccggc
      121 tcaggcgatg gactggctcg agtccgcgct caatccgaag cactcggtta cggcgatcct
      181 gcatgcgctg caggcgcggt aatgcgcttc aaggagtcgc tgcgcgcgac actctggccg
      241 tccacaagca cctgcaatcc gacgccccgg tcgtagcggg tccccgtccg gtcgtacagg
      301 atggtcagca tgcgtccatg gtaacgcacg ccgtcgaggc agaagtaatc ccacgtccca
      361 tccgacacga gcggattgac ctcgatgatg tcatcggggc gtggacgcag gccgaccagg
      421 ccggtgatca ccaagtcgca gaaggtcgag tgattgtagt ccttgccgcg ttcatcgggc
      481 ggctggcggc gttgtttgag cagagttcga gcgatccagt cgccggtgtt gggattgagg
      541 ttttcgtcga tccagggctg gcgtgtctcg atgcagtcga ggcgcacttc gccatcattg
      601 agagcgctgg gtttggctgc gtcgatgtcg ctgagggcgc gcgtgggccc aggctctgcc
      661 ggttgcgggg ggagttggcg aaaccggtgg ctgcgcagat agcactggaa ggtcttccag
      721 tagtcggctt tgccaatgat gtcttgggag tcgcggttga ggaggttcgc gagcgcggtg
      781 agcgtcacag aggttgccag aggccaactc gggccgttcc actggcattc gtgccctgcg
      841 taggcgactg agaaagacgg gtgtcgttgt tcggcagttg tgggaccgaa cggagcgtat
      901 agaagccatt ggaatccata agttcccgcc aggcaaccga catcgcgggt tcggggagat
      961 caaagtacca gggggtgtag ccgtgcagtc cgcggacgtc ggcaagcgtc gtcttgccgc
     1021 ggggcaggac cttgaagaag cgggcttcag ggtcccagag ctttgtgagc accagatggc
     1081 ggagggcgcc ggccttggcg cgccacgttt gggcgaggtc gtgctgatgg gcgcgttcgg
     1141 cgattcgtga gatggcgacg gcgtcgccgt actggtagct gttgatcgtg gcccggcagc
     1201 tgctgccgcc gatcgaaacc tccatgccat cattgccgtc gttttgccag aacagcccgg
     1261 tctcgtcgag atggctcttt tcccaggcct cgtagttggc gatcaggtcc ggcagcaact
     1321 cctgcgggat gcgatcgtcg ccgaggacca ggctgcgggc gtggatcgag tcagcggccc
     1381 agaagctgta cttgcgcggt tcgccgcctt tgcggaacca gaaaagagcg tagtcgtcaa
     1441 ggtattgcgg ctcgaggagc cagcggccct cgtagaagtg gtggccggcg gggcaattga
     1501 tcgagttgta tttcccggcc cagggcaccg gcggcaggaa ctccgtgatg atgaaaccgt
     1561 ccggtgtctt cttgatgtgc ttccgatagg tccaccatcg gaagtagtaa acctcttcga
     1621 gattcttgtc cgcgcactcg aaccgcggga tgttctgcga catcgactgc cacgccgccg
     1681 cgttccggat gtggttgacg accgtctcgc ggtcgttccg attgaacgac tcgacgtaat
     1741 gttgaaaggc atcggccttc aggactggtt ggccgggtgt ggggtcgggc gacgcctggg
     1801 cgaatggttc agccccgtga atctgggcca ggaccagggc gatgaatgca agccggcacc
     1861 aggccggaag cagcgggcgc cgtcggtcgg ggcgattcgt gggtggctcg atgccgggtg
     1921 ttccgagggg cgttggtccg ggtgccggcg cggtattcat cgcggttgat cagaccgccg
     1981 ccgtcggcat gaaacaaggc gaattcttga ccccttgtcc gcagcacaga aactcgagtt
     2041 gtctgcaacg agtctcaacg gtcggggttc ttgggcgcac ccccaggatc gaggaaatcc
     2101 tcccaaatct gggtggcggt gcgcatgcgg ttgaaaatgc gcaattcatc gaaggcggcc
     2161 accgcggcgc gggcgttgtc cgtttccgcc ccaatcgtca ggagctggtg ccgaacgagg
     2221 gcaggtggac gccgttgacc gcggtgcgga tcacctcacc gttctcgtag aaggcgagtt
     2281 cccgggtggt tcccctccag gcaacagcca gatgatacca ctgccctttt cgccagtgtg
     2341 aaccgtcgaa gcccaacccg cgctcgacat cgctctccac ggcgggcgtg cctggatcat
     2401 ctccccattg cgggaaccgc aggttgctcg ccccgtcttt cgagatgagc aggccgcgat
     2461 taaagttgtc gcccgcttca aggaacacgc ggacttctgt gtctgtcccg tcccagtcgg
     2521 gctggaccca gaattcgatc gtgccttcgt ccgattcgag attctggctg atgggataac
     2581 ggatatggcc ggtagcgtcc agatagacag cctgtcctgc ttggccgggg cggtaggagg
     2641 cgccgcgatg cgacaagggc ctttcgccgt ccgtgccgtc gagggtgcca ttaaacctga
     2701 gccacagcag gagagccgtt tcgtcggtgg gatgggttgc ccggcaaaag cgggtgcgcc
     2761 ctgttgtggt gcgatcgacg gccactgcgc cggtggcgct cagggtgaga tttgtaagca
     2821 gttgccaggg ggcggaaccg gccaggacat cggcgtattc gagtcggcag gacaggcccg
     2881 acgcgccatt gacctggagc acaggcgcgc gcgtgccggc ctccagggcg atatcgacgg
     2941 acaacgactc cgaggcgatc acggtgctgt cggcacacag catgaagaat gagacggcga
     3001 ggatcgcagg ggtgcgcatg gaaaaccgtt gaaccccgag gcgattcttc gaacaactat
     3061 ccatgagcgg atgctggcga acacggattg actgtcaaca gcgaatgccg ggcagaacac
     3121 cgtgcaaaac cgcgccatgt cgcagttcgg gccggggagc ctgtgcaccg ctcccagatc
     3181 gagatttccg tgccgcttgg aaggccgttc tccgatggca cggtttgcca ttctcaaggg
     3241 cgcgttcgcc ccgatcgttc acaggctccg cgctgaacct gatccgggct aatccgcatt
     3301 tgcccacatt ttggagtttt cagcttcgcc gtcgtcagga gtccatgaaa tatgccggtt
     3361 agttgtcgac gcggaggcgg taaaagcgct gcgcggccag gtcggtgtca atccaggaag
     3421 ccgagatgcc cgatgcgggt acagtggcga tcgttgtcca agcggcgccc tcgcccagtg
     3481 cggaggcgcg ttcgacgcga taggtgacgc cgggacttgc tgtccagcgc agttcgagtc
     3541 acctatcgcg tcgaacgcgc ctccgcactg ggcgagg
//
LOCUS       DAOQRO010000350         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_29119_length_3565_cov_4.488319, whole genome shotgun sequence.
ACCESSION   DAOQRO010000350 DAOQRO010000000
VERSION     DAOQRO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29119_length_3565_cov_4.488319"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKK20_11575"
     CDS             complement(<1..697)
                     /locus_tag="PKK20_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV00570.1"
                     /translation="MARPLRVLLEGGVYHVCFRGNERRVIFRDDTDRRRFLRQLKETV
                     DGHPVRVLLVCLMPNHVHLLIGTPRANLSRFMGRLLTAYAVYFNLRYHRVGHLTQGRF
                     KAQLVEGNEYLLRLSRYIHLNPVSGKRWLGISIQAKLEALRAYPWSSYRSYVGLEDPW
                     SVLDQGPILALVSGDRMSEAAEVYQSFVETGLVEDDATFALLYRRAKLSVGSELFNAS
                     VESRHRSLMARAFR"
     gene            complement(790..1581)
                     /locus_tag="PKK20_11580"
     CDS             complement(790..1581)
                     /locus_tag="PKK20_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00571.1"
                     /translation="MKAMYSRSAEERQAGFTLIELLVVIAIIAILAGMLLPALSRAKT
                     KATAIGCINNGKQLSLAWTMYANDYQDNLVANGDGTEDGWVGGWISVSNPINIRDCTN
                     INLLMPPHGKLYPYNQSLGIYKCPADKFLVVISGKKYPRTRSISMNGCMNGSSWHTDI
                     IKNKWWTYQKLSEITKPSTQFVFIDEREESVDDGYFLVMVDSPNQWGNLPAIYHNQAS
                     GLSFADGHAEIKRWMDPATITKNWSGAYIAPRDVPWINERASMLR"
     gene            2206..2847
                     /gene="clpP"
                     /locus_tag="PKK20_11585"
     CDS             2206..2847
                     /gene="clpP"
                     /locus_tag="PKK20_11585"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNV00572.1"
                     /translation="MNSFLIPMVVEQTGRGERGYDIYSRLLVDRIIFLGTPVDDMVAN
                     VVIAQFLFLQMNDPKKDIHLYIHSPGGSVTAGLAIYDTMQFLTCDVNTYCIGQAASMG
                     AVLLGAGTKGKRYALPNARIMIHQPWGGVQGQATDISIQAKEILRLKDRLNEILAHHC
                     GQSIESIARDTDRDRFMSADEAKGYGLVDEVVKSRKEIPSLAEGASAPAPAVQ"
     gene            2899..>3565
                     /locus_tag="PKK20_11590"
     CDS             2899..>3565
                     /locus_tag="PKK20_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV00573.1"
                     /translation="MSRPTQLTLCSFCGKSHAEVKKLIQGPGVFICDSCVLLCKSVLD
                     KELRTDSRHSLQALSVPRPADLKALLDQYCVGQEQAKKTLAVAVHNHYKRIQNQVRPR
                     GDAKAASPHDDVEIEKSNILLIGPTGCGKTLLARSLARALNVPFSIADATTLTEAGYV
                     GEDVENIVLRLLQNSDYDIERAQTGIIYIDEIDKIARKTENVSITRDVSGEGVQQALL
                     KILE"
BASE COUNT          718 a         1119 c         1039 g          689 t
ORIGIN      
        1 gtcggaaagc ccgggccata agacttcgat gccgactttc tacggacgcg ttaaacagct
       61 ccgatccaac actgagcttg gcccgacggt acaagagcgc gaatgtggcg tcgtcctcga
      121 ccaggcctgt ttcgacgaag gactgataga cttctgcggc ctctgacatc ctgtctccgg
      181 agaccagggc gagaatcggt ccctggtcga ggacagacca gggatcctcg agtccgacgt
      241 agctccgata actgctccag ggatacgccc tcaaggcctc cagctttgcc tggattgaaa
      301 tccccagcca tcgcttgcca cttacgggat tgaggtggat gtagcggctc aacctcaaca
      361 gatattcatt cccctcaacg agctgggcct taaatcgccc ttgtgtgagg tgcccaactc
      421 gatggtaacg caagttgaag tagaccgcat acgcggtgag aaggcgcccc ataaaacggc
      481 tcagattcgc tctcggcgtg ccgatcaaaa gatgaacgtg attcggcatg aggcatacca
      541 gaagcactcg aactggatgc ccatcaactg tctcttttaa ctgcctgagg aatcgtcgcc
      601 gatccgtgtc atcgcggaaa atgacgcgtc gctcattccc gcggaaacag acgtggtata
      661 cccctccttc aagcaggacc ctcaaaggcc ttgccatgag cccaacgtcg cacggtgctg
      721 gcctgtcgtc aacgcgtgga ttgctggcgt tccgcaaact tctaacttaa gggctgaccc
      781 tgccgcgctt cagcgcagca tgctggcgcg ctcgttgatc cagggaacgt cgcggggcgc
      841 gatgtaggcc ccggaccaat tcttggttat ggtcgcggga tccatccatc gtttgatctc
      901 ggcatggccg tcggcaaagc tcagtccgct ggcctggttg tggtagatcg ccggcagatt
      961 gccccattgg ttggggctgt cgaccatgac cagaaaatag ccgtcgtcca cgctctcctc
     1021 ccgctcgtca atgaacacga actgagtcga gggcttggtg atttcgctga gcttctggta
     1081 ggtccaccac ttgttcttga tgatgtctgt gtgccaactg ctgccgttca tgcagccgtt
     1141 catcgagatg ctccgggtgc gcggatactt cttgccgctg atcacgacca agaacttgtc
     1201 ggcggggcac ttgtagatgc cgagcgactg attgtaggga tagagcttgc cgtggggcgg
     1261 cattaggaga ttgatgttcg tgcagtcccg aatgttgatc gggttgctga cgctgatcca
     1321 ccctcccacc cagccgtcct cggtgccgtc gccgttggcc accagattgt cctgatagtc
     1381 gttggcatac atcgtccagg ccagactgag ttgcttgccg ttgttgatgc agccgatggc
     1441 cgtcgccttg gtcttggcgc gggacaacgc aggcaaaagc atgccggcga gaatcgcgat
     1501 gatcgcaatc accaccagca actcgatgag cgtgaaccca gcctgacgct cttcagcgga
     1561 tcgactgtac attgccttca taggtcgctc gtcttcacct gtaggccgct ccgccacacc
     1621 ccgccttgcc gaatcccctg cacggccgac ggcacttcac acgcggacat tgccgaacac
     1681 aaggcgaacc gtagactcga acccgcggat gctcaaggcc taatcgctgc caaactctgt
     1741 cgccgggaga ttcacccgcc tcatgcgcct gagatccttg gctgtcgatc gccgatcgcc
     1801 aacacccgct cggcgcatct gattgttgtc ggtgttctgt cgcctggtga aggcgccgga
     1861 ccgacaacgg atggagattg ccggaggggc gacgcctgcc tgccgcccgc cagacagcgt
     1921 ctgcaactcg catcgccgtg gacgaggacg tccccactcc agcgccttcg tgtcctcgaa
     1981 tagagcacgc gagatcgcgt gctctgcctg cgccgcaaag ggcagcggcc tgtctgccgt
     2041 gtcgggcacg gcacaggcag gcgcggcagg caggccccac gcggcgcctg ctcgattccc
     2101 accgataacc ttcggatgca ctgacacccg ccggtcggat gcaggcccgc ttgacgctcc
     2161 gggcaccgtc cgctacgttt cggcgcaagg tccgacgttt tgactatgaa cagtttcctg
     2221 attccaatgg tggtcgagca gaccggtcga ggcgagcggg gttacgacat ttactcccgc
     2281 ctgttggttg accggatcat ttttctcggc acaccggtgg acgacatggt ggccaatgtg
     2341 gtcattgccc agttcctttt cctccagatg aatgacccga agaaggacat tcatctgtac
     2401 atccattccc cgggcggcag cgtgacggca ggcttggcga tttacgacac catgcagttc
     2461 ctgacgtgcg atgtgaacac gtactgcatc ggccaggcag ccagcatggg cgctgtcctg
     2521 ctgggtgcag gcaccaaggg caaacgttac gccctcccca acgcccgcat catgatccac
     2581 caaccgtggg gtggcgtcca gggccaggcc acagacatca gcatccaggc caaggagatt
     2641 ctccgcctca aagaccggct caacgagatt ctcgcccacc attgcggcca gagcatcgag
     2701 tccatcgccc gcgacacgga ccgcgaccgg ttcatgtcgg ccgacgaggc caagggctac
     2761 ggattggtcg acgaggtggt caaatcgcgc aaggaaatcc cctccctggc ggaaggcgcc
     2821 agcgcgcctg cgcccgcggt ccagtaaacg agttggattg agagaactgc cagatcaccc
     2881 caaacaccga attcgcccat gtcgcgcccg acacaactga cgctttgcag cttctgcggc
     2941 aaatcgcatg cggaggtgaa gaagctgatc caaggcccgg gcgttttcat ctgcgacagt
     3001 tgcgtgctgc tgtgcaagag cgttctggac aaagaactgc ggaccgactc acggcattcc
     3061 ctccaggcgc tgagcgttcc gcgcccggcg gatctcaagg cgcttctgga tcagtactgc
     3121 gtggggcagg aacaggccaa aaagaccctc gcggtggcgg tgcacaacca ctacaagcgg
     3181 attcagaacc aggtcaggcc ccggggagat gccaaagccg cgtctcccca cgacgacgtc
     3241 gaaatcgaga agagcaacat cctgctcatc ggtcccacag gctgcggcaa gacgctgctg
     3301 gcccgatcgc tggcgcgcgc cctcaacgtc ccgttctcga tcgccgatgc cacgacgctg
     3361 accgaggccg gttatgtggg cgaggacgtc gagaacatcg tcctgcgcct gctccagaac
     3421 tcggactacg acatcgagcg cgcgcagacc ggcatcatct acatcgatga gatcgataag
     3481 atcgcgcgga agacggaaaa tgtctcgatc acgcgcgacg tctccggcga gggcgtccag
     3541 caggcgctgc tgaagattct ggaag
//
LOCUS       DAOQRO010000351         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_29181_length_3560_cov_4.553495, whole genome shotgun sequence.
ACCESSION   DAOQRO010000351 DAOQRO010000000
VERSION     DAOQRO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29181_length_3560_cov_4.553495"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKK20_11595"
     CDS             <1..426
                     /locus_tag="PKK20_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00574.1"
                     /translation="VFAYATTQDWPEFLGIREDVFLRVMDVVKESGTGFAFPSQTLYF
                     ARDNGLDVAQAQAAEARVRQWREQGCLPFPDFAPEQERQMQGSIVFPPPGSPASPRGR
                     SASGAVSSSSSDARGAKAGTEPGSSASSGAIFDPISGAG"
     gene            complement(583..3444)
                     /locus_tag="PKK20_11600"
     CDS             complement(583..3444)
                     /locus_tag="PKK20_11600"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HNV00575.1"
                     /translation="MGLLEKADWQARWIADPVSVTNILLSGPRNGYHSQLATNASTTK
                     WIALDLGQPVEFDAIALFPARPYDWQPDTPGFLFPLRWRLEAAQTADFSDARLLVDRT
                     GLDEPNPGASPAIVRFPATTARHVRLTATRLRERDAGQHGLALAEFEVLRGQDNLAAR
                     ASVTALDSIETGAWAMANLTDGATVTLKPGDVPGALPATLVRKSFQIQGAVKRSTAYA
                     TACGLYELRLNGQRAGDQLLMPEWTSYRKRIQYQVFDVTHLLQPGTNALAAALAEGWY
                     AGRLMAVGRNAYGSHPRFLLQLEIELADGRVQRIVTDESWRSTTDGPIRAAGIYDGEV
                     YDARKESDGWDEPGFNDRDWRPARVFDLGDVQLVWQRNEPIRVVRELHPIAQTEPRPG
                     VHVFDLGQNMVGWCRIAVRGSAGQDITIRHAEMLNDDGTLYTANLRGAQQIDRYIPRA
                     DGDVVYEPRFTYHGFRYVELTGLDKPPARGAVLGRVFHSTSPETGGFECSNPSLNQLM
                     RNIVWTQRANLMSSPNDCPQRDERFGWMGDIQAFSQTAIFNMDLAAFLTKFAQDIRDD
                     QADDGRYPDFAPHPGDPNSSFSGVPAWGDAGTVIPWRLFENYADTRLLEQHFESARRW
                     VEWIRRQNPDLIWTKGRHNDYNDWLNGDWIRQENWPNKGGAVPKEVFATAFFAHSTEI
                     VARMAAQIGRPDQARQYHELFEQIRAAFNRRFVSPDGHIEGGTQAGYALALNFDLLPE
                     SLRPEAARNLVDEIRRYQVHLSTGIQTTHRAMLELTRHGHADLGWQLLTNRTFPSWLY
                     MIDNGATTVWERWDGYVKGRGFQDAGMNSFNHWAFGAVGEWIWRHVVGLNPDTACPGW
                     KHFLIAPRPGGGVAWARGHYDSIRGRIACHWTIEDGRFRLQATVPPNTTATIRVPTSD
                     ASRITESGQSAQPSRVDENAALFEVEAGTYSFVSPFEAP"
BASE COUNT          594 a         1077 c         1236 g          653 t
ORIGIN      
        1 gtgtttgctt atgcgacgac ccaggactgg cccgagtttc tcggcattcg agaagacgtg
       61 ttcctgcggg tcatggacgt ggtgaaggag agcggcaccg gcttcgcttt cccgtcgcag
      121 actctctatt tcgctcgcga caacggcctc gacgttgcgc aggcgcaggc ggctgaggcc
      181 cgcgtgcgcc agtggcgcga acagggttgc ctgccgtttc ccgacttcgc gcccgagcag
      241 gaacgacaga tgcagggatc cattgtgttc ccgccgcctg gctcgccggc ctcgccccgt
      301 ggccgatccg cgtccggggc ggtttcctca tcttcttccg acgcccgggg ggcgaaggca
      361 gggacggaac cggggagttc cgcctcatcc ggtgcaatct tcgatccgat ctcgggcgcg
      421 gggtgatggc tctctggagt gagccgcgtt acgagaacgg cgcgttgcgg ttcgctgtca
      481 tgcacagcgc gaaccgcgag cggcacgccc tgcggatggt tgcccgcctg cggaagggcg
      541 ccgccgagaa tctggtcttt cacaccggcg ctgcgaaccg attcatggcg cctcgaaagg
      601 cgacacgaag ctgtaggtgc ccgcctcgac ctcgaacagg gccgcgttct cgtcgacgcg
      661 actcggctgg gcactttggc cgctttccgt gattcgcgag gcgtcggacg taggcacgcg
      721 aatggtagcc gttgtattgg gcggcacagt ggcctgtagc ctgaatcgac cgtcctcgat
      781 cgtccagtgg caggcgatgc gtccgcgaat cgaatcgtaa tggccgcgcg cccacgcaac
      841 gccgccgccc ggccggggcg cgatgaggaa gtgtttccag ccgggacatg cggtatcggg
      901 gttgagtccg acgacatgac gccaaatcca ttcgccaacg gcgccgaagg cccaatggtt
      961 gaaggagttc atgccggcat cctggaagcc gcggcccttg acgtagccgt cccagcgttc
     1021 ccagaccgtg gtggcgccat tgtcaatcat gtagagccag gatgggaagg tccggttggt
     1081 cagcagttgc caccccagat cggcatggcc gtgccgggtc agttccagca tggcgcgatg
     1141 ggtcgtctgg attccagtcg agaggtggac ttggtagcgt cggatttcgt ccaccagatt
     1201 ccgggcggcc tcggggcgga gtgactccgg caggaggtcg aagttgaggg cgagggcgta
     1261 accggcctgg gtgcccccct cgatatgacc gtcgggactc acaaatcgac ggttgaaggc
     1321 ggcccggatt tgttcgaaaa gctcatggta ctgccgcgcc tggtcaggcc ggccgatttg
     1381 ggcggccatt cgagcgacga tctccgtcga gtgcgcgaag aaagcggtgg cgaacacctc
     1441 tttgggcacg gcgccaccct tgttcggcca gttctcctgg cgtatccagt cgccgttcaa
     1501 ccagtcgttg tagtcgttgt gccgcccctt ggtccagatc aggtccgggt tctgtcggcg
     1561 aatccactcc acccaccggc gtgcggactc gaagtgctgt tcgagcagtc gggtgtcggc
     1621 gtagttctcg aacaagcgcc acgggatgac ggtgcctgcg tcaccccacg ccggcactcc
     1681 agagaaactc gaattcggat cgcccgggtg cggcgcgaag tcgggatagc gtccgtcgtc
     1741 ggcctgatcg tcgcggatat cctgggcgaa cttggtgagg aacgcggcga ggtccatgtt
     1801 gaagatagcc gtctgggaaa aggcctggat atcgcccatc caaccgaacc gctcgtcgcg
     1861 ctgcggacaa tcgttgggtg aactcatcag attggcgcgt tgcgtccaga cgatgttgcg
     1921 catgagttgg ttgagcgagg gattcgagca ctcgaagccg cccgtctcgg gcgaggtcga
     1981 gtggaagacg cgcccaagga ccgcgccgcg ggcggggggc ttgtcgagtc cggtcaactc
     2041 gacgtatcgg aagccgtggt aagtgaagcg cggctcgtag accacgtcgc cgtcggcgcg
     2101 cgggatgtaa cgatcgatct gctgggcgcc acgcaagttc gccgtataaa gcgtgccatc
     2161 gtcgttgagc atctcggcat ggcggatggt gatgtcctgg ccggccgaac cgcggacggc
     2221 gatgcggcac cagccgacca tgttctggcc gaggtcgaag acgtggaccc cgggacgagg
     2281 ctcagtctga gcaatcgggt gcaactcccg cacgacgcgg atcggttcgt tgcgttgcca
     2341 aacaagttgc acatcgccca ggtcaaagac tcttgccggg cgccaatcac ggtcgttgaa
     2401 tccgggctcg tcccagccgt ccgactcttt gcgagcgtcg tagacttcgc cgtcgtagat
     2461 accggccgcg cggatggggc catcggtcgt actgcgccag gactcatcgg tcacgattcg
     2521 ttgcacgcgg ccgtcggcca gctcgatttc gagttgcagg aggaatcgcg ggtgcgatcc
     2581 atacgcgttg cggcccacgg ccatcaggcg gccggcgtac cagccctcgg ccaaggcggc
     2641 ggccagagcg tttgtgcctg gctgcaacag gtgggtgacg tcgaagacct ggtactggat
     2701 gcgtttgcgg tagctggtcc attcgggcat cagcaactgg tcgccagcgc gctggccgtt
     2761 caggcgcagt tcatagaggc cgcaggcggt ggcataggca gtcgagcgtt tgaccgcgcc
     2821 ttggatctgg aacgacttgc ggaccagtgt ggcaggcagc gcgccaggaa cgtcgcccgg
     2881 cttcaaggtc actgttgccc cgtctgtaag gttggccatg gcccaggccc cagtttcaat
     2941 cgagtcgagc gctgtgacgg acgctcgcgc ggcaaggttg tcctggccgc gcaaaacctc
     3001 gaactccgcc agggccagac cgtgctggcc agcgtctcgc tcgcgcagtc gagtggcggt
     3061 caggcggaca tggcgcgccg tggtcgcagg gaaacggacg atcgcggggc tggcgccagg
     3121 gttgggctcg tcgagtccgg tgcggtcaac gagaagtcga gcgtcggaga aatcggccgt
     3181 ttgagccgct tcgaggcgcc agcgcagcgg gaacagaaag cctggcgtgt cgggctgcca
     3241 gtcgtacggc cgggcgggga acaaggcgat ggcatcgaac tcgacgggtt ggccaaggtc
     3301 gagggcgatc catttggtcg tgctggcatt ggtggccagt tggctgtggt aaccgttgcg
     3361 gggtccgctc aacaggatgt tcgtgacgct cacggggtcg gcgatccagc gggcttgcca
     3421 atcggccttc tcgagcaagc ccattgtcca tgtggatgct gagctccacc cgcagggtcg
     3481 gcccgccctg tcccagacat ggactttcca ccagacgcgc atacgggagc gaaggggctt
     3541 cccgacgtac gccacgtgca
//
LOCUS       DAOQRO010000352         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_29509_length_3534_cov_5.051739, whole genome shotgun sequence.
ACCESSION   DAOQRO010000352 DAOQRO010000000
VERSION     DAOQRO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29509_length_3534_cov_5.051739"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKK20_11605"
     CDS             complement(<1..336)
                     /locus_tag="PKK20_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV00576.1"
                     /translation="MERAVGLFVFLATLLLVAGFVYYVYQTAKRKGWLVTKVEYCTSL
                     YSAAGLKVGDPVKLMGFDVGEITLIDTESPDGVYNVYVQFSVRAPYFGYLWTAGSKAR
                     VSSDFLGKRT"
     gene            complement(382..1563)
                     /locus_tag="PKK20_11610"
     CDS             complement(382..1563)
                     /locus_tag="PKK20_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00577.1"
                     /translation="MNRQVTSGLRGAVLGLVVGAVAAAGWAAEVPGAPDKVTFSDSED
                     EGKGGARVDVAVPTPMFQRRVDAPRMGGSSLEGVRAIPFAPPSSLSPSVDSRRLRDAR
                     SNWILDPGKNLDLNKDNAFRAFGVRSGVDFEKFGEQSDRPMNFWEAYFSKEKADAKQS
                     RSPVKLDPMPSERMNRGELTPRFQPFQAFGSTEFDGFAPLSIDRERTGVLFSDTPSRS
                     SILGTLGDALDSNSRRDLVRDSLNLTTMPPGAKPVGGAEARIEQFRRMLGIGLPTDEK
                     PAASRAGVASVLSESGPNESMVESMDLIGVIADPTRRELNPVVARPAPGFDAGPKLEF
                     VDTIGGVNSRPAAQRRPLIDMSPVRSVLPATASADRNTGTAAPRTIYSPTTLEMPGRN
                     F"
     gene            complement(1647..>3534)
                     /gene="dnaE"
                     /locus_tag="PKK20_11615"
     CDS             complement(1647..>3534)
                     /gene="dnaE"
                     /locus_tag="PKK20_11615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV00578.1"
                     /translation="GLLKMDFLGLKTLTVIRNTCEMVRQTQGVAVPIDQLPLDDAATY
                     DLLNKANTLGVFQLESDRMRDLCRKFQISSIEHITALIALYRPGPMDLIPDFIRRRHG
                     ETAIEYAHPLLEPLCRETYGVMVYQEQVMQAAQVLAGYTLGAADLLRRAMGKKKAEEM
                     AKQRSIFVRGCKEKNGIAADRANQIFDQLEKFAGYGFNKSHAAAYAVVAYQTAYLKAN
                     YPVEFLCAMLTNDMADTDKLSLYIGEARAFGIEVLGPDVNESQIHFAPGGRDEDAGGR
                     KSIRFGLAAIKGVGEMAVASILKAREEGGAFESLADLCDRVDLRAVNRKVLEALVKSG
                     ACDTFGETRATLWAAIDGALARAASAAQDRARGQTSLFGLLEDQPAALGESIARVQEW
                     PQSELLAAEKELLGFYVSGHPLTPYADLLQQFALATSSVLAQLPHRTMTRIGGLITVA
                     QHGISKKTNKPYALATIEDLTGSMQLVCLNESYEKHKALLQPNTAVLVVGEVNTTDEK
                     PKVFAHEVILLDDAPQRYTEQVHFRLHTAHLNAERLEKARALATDHAGRCPLFLCLIH
                     PAGELVFIEAHDRYRVTPSLALQRAADALFGENTYYAKIDRTAPPRAARRWERSETSG
                     VNGE"
BASE COUNT          654 a         1218 c         1020 g          642 t
ORIGIN      
        1 agtgcgcttc cccagaaaat cggaactcac ccgcgccttc gatccggccg tccagagata
       61 cccgaaatac ggggctcgga ccgagaattg gacatagacg ttgtagaccc cgtcggggct
      121 ctcggtgtcg atcagggtga tctcgccaac gtcgaatccc atcagcttca ccggatcgcc
      181 caccttgagg ccggcagcgc tgtacaggct ggtgcagtac tcgaccttgg tcaccagcca
      241 acccttgcgc ttcgccgtct ggtacacata gtacacaaag ccggccacca gaagcagcgt
      301 cgccaggaaa acaaacaacc caaccgctcg ctccacgcga cccagacgcg tccgcagttg
      361 aggcgtaaga tcctgcactc gctagaaatt ccgcccgggc atttcgagcg tcgtcggcga
      421 gtagatcgtc cggggagccg cggtccccgt gttgcgatcc gcactggcgg tggcaggcag
      481 cacgcttcgc acgggactca tgtcgatcag cgggcggcgc tgggccgctg ggcgcgagtt
      541 cacgccgcca atcgtgtcga caaactccag cttcgggccc gcgtcaaagc ccggggcggg
      601 gcgagcgact accggattca gctcccgtcg ggtgggatcc gcaatcacgc cgatcaaatc
      661 catcgattcc accatactct cgttcgggcc ggattcggag agaaccgagg ccacgccggc
      721 acgcgaggcc gccggctttt catcggtcgg cagaccgatt cccaacatgc gccgaaactg
      781 ctcgatccgc gcctcggccc ctcccaccgg cttcgcaccc gggggcatgg tcgtgagatt
      841 gaggctgtcg cgaaccaaat cccgccggct attgctgtcg agcgcgtcgc ccaaagtgcc
      901 gagaatcgag ctcctcgatg gagtgtccga aaacaacacc cctgtgcgct cccggtcgat
      961 cgagagcggg gcgaagccgt cgaactccgt tgacccgaaa gcctggaaag gctggaaacg
     1021 cggtgtcaat tcgcccctgt tcatccgctc gcttggcatt ggatcgagct tgaccggcga
     1081 gcggctctgc ttggcgtcgg ccttctcctt gctgaagtac gcctcccaga aattcatggg
     1141 ccggtcgctc tgctcgccaa atttctcgaa atcaacgccg gaccggaccc cgaaggcccg
     1201 gaaggcattg tctttgttga gatcgagatt cttccccgga tcgagaatcc agttgctccg
     1261 cgcgtcgcga agccgtcgcg agtccacgct gggcgacagg ctcgatggcg gggcaaacgg
     1321 aatcgccctg acgccctcaa gagaactgcc tcccatccgc ggcgcatcca cgcgccgctg
     1381 gaacatggga gtcggcaccg ccacatccac gcgcgcgcca cccttgccct cgtcttcact
     1441 gtctgaaaag gtgaccttgt caggcgcgcc cggaacctcg gcagcccaac cggcggcggc
     1501 tacagccccc accacgaggc cgagcaccgc cccgcgcaaa cccgatgtca cctgtcgatt
     1561 catgtcctgc tcaattctta cgcccgaaga ggaagaccgt caaagggctt ttcccgccca
     1621 ttcgacccca aatcgggctg tttcgttcat tcgccgttga cgccggaggt ctcgctgcgc
     1681 tcccaccgcc gcgccgcgcg gggcggcgcc gtgcgatcga tcttggcgta gtaggtattc
     1741 tcgccgaaaa gcgcgtcggc ggcccgctga agggcaagcg aaggcgtgac acgatagcga
     1801 tcgtgcgcct cgatgaagac cagctcgccc gccgggtgga tcaggcacag gaacaggggg
     1861 caacggcccg cgtggtcagt ggccagcgcc cgcgccttct cgagacgctc ggcattcaga
     1921 tgcgccgtgt ggagccggaa atgcacctgt tcggtgtagc gctgcggggc gtcatcgagc
     1981 aggatcacct catgcgcaaa gaccttcggc ttctcgtcgg tcgtgttcac ctcgcccaca
     2041 accagcacgg ctgtgttggg ctgaagcaac gccttgtgtt tctcgtagct ctcgttcaag
     2101 cagaccagtt gcatcgatcc ggtcaagtcc tcgatcgtgg ccaatgcgta cggtttgttc
     2161 gtctttttcg aaatgccgtg ctgcgccacg gtgatcagcc ctccaatccg ggtcatcgtc
     2221 cggtgcggca actgggccag cacactcgaa gtcgccaggg cgaactgctg cagaagatcc
     2281 gcgtaaggcg tcagcggatg gcccgagaca taaaacccaa gcagttcctt ctccgccgcc
     2341 agcagttcgc tctgcggcca ttcctgcacc ctggcgatgc tctcgcccag cgcagccggc
     2401 tggtcttcga gcaaaccgaa aagcgaggtc tgcccgcggg cgcggtcttg ggccgcgctc
     2461 gccgcccgcg ccaatgcccc gtcgattgcc gcccacaggg tcgcccgcgt ctcgccgaac
     2521 gtgtcgcagg ctcccgattt caccagagcc tcgaggacct tgcgattgac tgcccggagg
     2581 tccacgcgat cgcaaaggtc ggccagcgac tcaaatgccc ccccttcctc ccgggccttg
     2641 agaatgctcg ccacggccat ctcgccaacg cccttgatcg ccgcaagacc gaatcggatc
     2701 gacttgcgcc cgcccgcgtc ttcgtcgcgc ccgccgggag cgaagtgaat ctggctctcg
     2761 ttcacgtcgg gtccgagcac ctcgatccca aaagcccgcg cctcgccaat gtaaaggctc
     2821 agcttgtcag tgtccgccat gtcgttcgtc aacatcgcgc acaggaactc gactggataa
     2881 ttggccttca gatacgcggt ctgatacgcc accaccgcat aggcggccgc gtgcgacttg
     2941 ttgaagccgt agccggcaaa cttctcgagc tgatcgaaaa tctggttggc tctgtccgcg
     3001 gcaatcccat tcttttcctt gcagccccgg acgaagatcg aacgctgctt ggccatctcc
     3061 tcggccttct tcttgcccat ggcgcgccgc aacaggtccg ccgctcccag cgtgtagccc
     3121 gccagcacct gcgccgcctg catgacctgc tcctgataca ccattacccc ataggtctcc
     3181 cggcagagag gctcgagcaa aggatgcgca tactcgatcg ccgtctcccc gtgccgcctc
     3241 cggataaagt cagggatcag gtccatcggc cccggacgat acagcgcgat caacgccgtg
     3301 atgtgctcga tcgaactgat ctggaacttc cggcacaggt cgcgcatgcg gtccgattcc
     3361 aattggaaca cgcccaatgt gttggccttg ttcaggaggt cataagtcgc cgcgtcatcc
     3421 agcggaagct ggtcgatcgg aacggccacc ccctgcgtct gccggaccat ctcgcacgtg
     3481 ttgcgaatga cggtcaacgt ctttaaaccc agaaagtcca tcttcagcag accc
//
LOCUS       DAOQRO010000353         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_29751_length_3514_cov_4.752241, whole genome shotgun sequence.
ACCESSION   DAOQRO010000353 DAOQRO010000000
VERSION     DAOQRO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29751_length_3514_cov_4.752241"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKK20_11620"
     CDS             <1..593
                     /locus_tag="PKK20_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNV00579.1"
                     /translation="EGVEIIGVILNKVLPEKLSHVEKFAGKGFRRQGLELLGVLPHQH
                     ILTSPTLGLIREELGAESLNPSPQDSNLVEEVVVGAMSVRQALKSFKKGVLVITPGDR
                     EDIVLAAATTLFGQGTDGLAGILLTDNQRPSPNVMRVIAALPFPVLLTKEESYRVASR
                     VHDLTVKTRPDDTAKILLIRDLIAQHVNVQKILESI"
     gene            616..1725
                     /gene="hisC"
                     /locus_tag="PKK20_11625"
     CDS             616..1725
                     /gene="hisC"
                     /locus_tag="PKK20_11625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNV00580.1"
                     /translation="MNASTSLLLNPYLEHLPVYQPGRPIDEVARELGLPADDVIKLAS
                     NENPLGPSPKALAAMQQSLRSLHLYPDGNAFYLKRHLADKLRVAPENLILGNGSNEVI
                     EFVGHALIMPGSEVVVSEFCFAVYPIVTHLCGGKLVSVPAREYGHDLPAMRRAITPRT
                     AVVFVANPNNPTGTLASDDEVLELIHEAPPHVLVVLDEAYIEFRDRPLDLLELIRSGS
                     KPNLLLTRTFSKIHGLAGLRLGYGIGGAEIIAACEKVRQPFNINTMAQVAALAALDDE
                     DHQRRTKENNAVGLRFFEEAFRALGLLFVRSHANFVLVKVGDGQRVFNDLQQQGVIVR
                     PMGGYRLPEWIRITVGTPAENQRYLASLKQALGRA"
     gene            1789..1986
                     /locus_tag="PKK20_11630"
     CDS             1789..1986
                     /locus_tag="PKK20_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00581.1"
                     /translation="MKRPALASQLQPGESVQLEDSPTPLVLTRQRLPRLSAQDIERDL
                     AALAQGIAPMDADTLLEDLRS"
     gene            1983..2432
                     /locus_tag="PKK20_11635"
     CDS             1983..2432
                     /locus_tag="PKK20_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00582.1"
                     /translation="MTIYPDTSFLVSFLAGWERHHALAMAWFRPRSEAEWLISPWAEF
                     ETLNILRQMCREPNGPPPNRVEAVRRYWRHLFRAGPFVRRPVDWLATTAGCQELSAAF
                     SVRQRMRSADTLHIAILDQILPDLFVTSDGNQHALALARGFESEKYR"
     gene            complement(3132..3338)
                     /locus_tag="PKK20_11640"
                     /pseudo
     CDS             complement(3132..3338)
                     /locus_tag="PKK20_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
     gene            complement(3390..3476)
                     /locus_tag="PKK20_11645"
                     /pseudo
     CDS             complement(3390..3476)
                     /locus_tag="PKK20_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          645 a         1122 c         1103 g          644 t
ORIGIN      
        1 gcgagggcgt cgagatcatc ggtgtcatcc tcaacaaggt gttgcccgaa aaactcagcc
       61 acgtcgagaa gttcgccggc aagggattcc gacggcaggg actcgagttg ttgggggtgc
      121 tgccgcatca gcatatcctg accagtccga cgctcgggct gattcgcgag gaactggggg
      181 ccgagtcgct caacccgagt ccgcaggact cgaatctggt cgaggaagtg gtggtcggcg
      241 cgatgagcgt tcgacaggcg ctgaaatcgt tcaagaaggg cgtgctggtc atcacgcccg
      301 gcgatcgcga ggatatcgtg ctggcggccg ccacaacact cttcgggcag gggaccgacg
      361 gcttggccgg cattctcctc accgacaacc agcgcccctc gccgaacgtg atgcgcgtca
      421 ttgccgctct gcccttcccg gtcctcctca ccaaggagga gagctaccgg gtcgcgtccc
      481 gtgtccacga cctcacggtg aagacgcgtc cggacgacac cgccaagatt ctgctcattc
      541 gtgatttgat tgcccaacac gtcaatgtgc agaagatact cgaatcgatt tgagcagata
      601 caataacacg atcctatgaa tgcatcgact tctctcctct tgaaccctta cctcgagcat
      661 ctgcccgtct atcagccggg ccggccgatc gacgaagtgg cgcgcgagtt agggctgccg
      721 gccgacgacg tcatcaagct cgcgtcgaac gagaacccgc tgggaccttc gcccaaggcg
      781 ctggccgcga tgcagcagag cctgcgatcg ctgcatctct atcccgacgg caacgccttc
      841 tacctcaaac ggcatctggc ggacaagctg agggtcgctc cggaaaacct gatcctgggc
      901 aatggctcga acgaagtgat cgagtttgtg ggccatgccc tcatcatgcc cggatcggag
      961 gtggtagtct ccgagttttg tttcgcggtc tatccgatcg tgacgcatct gtgcgggggc
     1021 aagctggtgt ccgtgccggc gcgcgagtac ggccatgacc tgcccgcgat gcgtcgggcc
     1081 atcacgccgc gcaccgccgt ggttttcgtg gccaatccca acaatcccac cggcacgctg
     1141 gcctcggacg acgaggtcct cgaactgatt cacgaggcgc cgcctcacgt gctggtggtc
     1201 cttgacgagg cgtacatcga gtttcgagat cggccgctcg atttgctcga actgatccga
     1261 tcgggctcaa agccgaacct cctgctcacg cggacattct cgaaaatcca cggtctggcc
     1321 ggactgcggc tgggatacgg gattggcggc gcggagatca ttgccgcctg cgagaaagtg
     1381 cggcagcctt ttaacatcaa cacgatggcg caggtggcgg ccttggcggc actcgacgat
     1441 gaggatcatc agcgccggac taaggagaac aacgccgtcg ggttgcgctt tttcgaagag
     1501 gcgtttcgcg cgttggggct gctcttcgtg cgttcgcatg ccaatttcgt cctggtcaaa
     1561 gtgggcgacg gtcagcgcgt gttcaatgat ctgcagcagc agggcgtcat cgtgcggccc
     1621 atgggcggct atcgtctgcc cgagtggatt cgaatcaccg tcggcacgcc cgcggagaac
     1681 cagcgttacc ttgcctcttt gaagcaggcc ctgggacgtg cgtgagcggg tgctgcctcg
     1741 gttgcgatcc ttgacgagac cctgccgaga ccattcaaac gttcgttggt gaagcgtccc
     1801 gcgctcgcct cgcagttgca acccggcgaa agcgtccagt tggaggattc gcccacaccg
     1861 ctggttctga cacgccagag actgccacga ctctcggcgc aagacatcga acgtgacctg
     1921 gccgccctcg cgcaaggcat cgcgccgatg gatgccgaca cgctcctgga ggacctccgg
     1981 tcgtgacgat ctacccggac acttcgttcc tcgtgtcgtt cctggccggc tgggagcggc
     2041 atcacgcgct ggccatggct tggtttaggc cacgcagtga agcggaatgg ctcatctcgc
     2101 cgtgggcgga gttcgaaacg ctgaacatcc tgcggcagat gtgccgcgag ccaaacggcc
     2161 cgccgcctaa ccgggtcgag gcggtgcgac gctactggcg gcacctgttc cgagcggggc
     2221 cgtttgttcg tcggccggtg gactggctgg ccacgacggc cggctgccaa gaactcagcg
     2281 cggcgttcag cgtgcggcag aggatgcgct cggcggacac gctgcacatc gccatactgg
     2341 accagattct gcccgatcta tttgtgactt cggacggcaa ccagcacgcg ttggcgctcg
     2401 cccgcggctt cgagtcggag aagtaccgtt agccggctct tcacgagcgg aattcgggtc
     2461 ataactcgac atctggcaat gggcgcggcc tcagggtggc gctggtactc gcggatgacc
     2521 gtcgtcaagg tgaggcaagg cgctggtgaa ttctaattgt cgtttggcag caagcagcct
     2581 gaacgattgc agtgcatgga acattctccc tacccaggga gattccgccg ccgagaggcg
     2641 gcgggaagtc agcagaggcc atagttgcag aaaactcatc tgcaggtgaa cccgcttgac
     2701 actgttattt atttacgccg ccttattcga gaaacccgga atggcgcaac gctacgcgca
     2761 gtcattttga cccgggccaa accagaccag tttccctttg acgtcccagc cgtagcgatt
     2821 ctccacgccc cagttctgaa accggcgagc cgatgaagac accagtgctc ttcttggcga
     2881 cgcgacaatg aggcaacgtc accccttgtc cgtgggaagc gggggcgggc tcttcggctt
     2941 tctcgcgggg gctttccagc agccgcccgt gctgaattga atccaggtcc atcgcagcca
     3001 acggattagc gccgcggcga gccagagggc ccaggccagc atcagcaacc ggtagaacca
     3061 gactgagacc gacaccacga ccggcagggg cagttcggtc ccgctgcgcg gctggaacca
     3121 gcgtaactcg agccgcgacg aaccgtttcc gcgatccgcg cttccttgga acccgtgacc
     3181 agatggtcga ccaacacccc gagacgccgc cccgggcccg gcttgaattc ggcgacgatg
     3241 tcgacgaggt cgtcgacacc gcccaacggc tcgacgacga cgccttcgac ccgaaggtcg
     3301 tggccccaca cctgctcgac cagttccgcg tcgtgccggc gcagggcgta cttgacgatg
     3361 gcgagtccga ggccggtgcc gccccgctgc cgggcccgcc cgccgtcggc ccggtagaag
     3421 cgctccgtga cccgggggat gtcctcctcg gcgatgccga tgcccgtgtc ctgcacggcc
     3481 aggtgcccgc cgtcgccgtc gacggaccag cgga
//
LOCUS       DAOQRO010000354         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_30138_length_3483_cov_4.607351, whole genome shotgun sequence.
ACCESSION   DAOQRO010000354 DAOQRO010000000
VERSION     DAOQRO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30138_length_3483_cov_4.607351"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1759
                     /locus_tag="PKK20_11650"
     CDS             86..1759
                     /locus_tag="PKK20_11650"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNV00583.1"
                     /translation="MFAAIRKHQTWLWGFIIAAVIVSFVIYFTPMARQGRGGGGRRSQ
                     FGTLNGRPISRQQYIDALGDVRLSYFLRFGSWPDDADARRMGFNVGQETQNRLVLIDR
                     LDALNIQVSESAVAQWIIDNFGGDQPATAKAKYDNFVAELRQRHGVSEQALQGFIRRD
                     IGINHLAEIAGLPGKLITPRQATELFQEENEKIVAEAVVFPASNHIASVQLDPAAIGQ
                     FYSNRLSVYRTPDRVQLHYLRFDVTNYLAQAEAALARRTNLAAEIERIYLSSNPNAFV
                     DTNGQVMAPEAAKERILNQTQRQQSLAEAHKAAALFASAFETMTNLTADTFVAQAAEK
                     GLVTALTQPFAETESPQGLRVRENFVSMAFKLTPEYPVSLSPIRGEDAVYLIALKQRI
                     PSQIPALEDIRTRVEQEYIQDQAVRLAREACTNFVIQLTNGLAQGKTWAAIHAEAGLT
                     PIALPKFGVASGPVPNWDRQVDLNQARRAVRTLDAGRPTDAISTRDGGMVLYVKERVP
                     VTDAELKEELPGYLAKLRQTSEYQAFNDWFRKQVEQSRIDTGLAKEETD"
     gene            1769..2572
                     /locus_tag="PKK20_11655"
     CDS             1769..2572
                     /locus_tag="PKK20_11655"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV00584.1"
                     /translation="MTSPIKLSSRSTHEYWEIPVLFEDDSLLALDKPAGLLTSPDRNE
                     PARPNLMALLHAGIRAGAPWARTRGLAYLAHADRLDADTSGVLLLAKSKQILITLANH
                     LGSGTPAKTFVALVQRAPAKAAFDIIAKLAPDPRQPGRMRVDAQRGKKSITACLVEES
                     FVGYTLLRCSPHTNRSHQIRAHLWSQRLPIVGDSLYGGQPLWLSRLKRAYRLKPGRQE
                     RPLMGRVALHAASLNLPHPVTGENIEIVSPWPKDLRVAVKYLRQFAPAA"
     gene            2867..>3483
                     /locus_tag="PKK20_11660"
     CDS             2867..>3483
                     /locus_tag="PKK20_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00585.1"
                     /translation="MKLPKLKITWAALLTAVAVVSARADYPGTVLSQGPAAYYRLNET
                     TAPPPLTLQAVNIGSVGAAGNGEFRNGVERAVAGAIVSDPSNLAVYMPGAEGNRVRIP
                     WNEAWNSETAISVEFWAKPGQTNELACPAASVDFITGPPAQRNGWLFYQGDTTLTGGN
                     GWLFRYYNNSSLTAVSGAAVTMDLSTDQWYHVVGVYDGTNIKIYV"
BASE COUNT          692 a         1176 c         1006 g          609 t
ORIGIN      
        1 aagcagattc gccgattgca gggcgttgct tttgcgtttg accgtcagct tgcgaggcgg
       61 atacgttgtc gctttgcttt gacctatgtt tgccgcgatt cgtaaacatc agacttggct
      121 gtggggcttc attatcgccg cggtcatcgt cagctttgtc atttacttca cgcccatggc
      181 cagacagggc cgcgggggag gcggcaggcg atcccagttc gggacgctca acgggcgccc
      241 gatctcccga cagcagtaca tcgatgcgct cggcgatgtc cgcctttctt acttcctgag
      301 gttcggctca tggccggacg atgcggacgc gcggcgaatg ggcttcaatg ttggacagga
      361 gacgcagaac cgcctggtgt tgatcgatcg gctcgatgct ctcaacatcc aggtcagcga
      421 atcggcggtg gcccagtgga tcatcgacaa tttcggcggc gaccagccag ccacggccaa
      481 ggcgaaatac gacaacttcg tcgccgaact ccgccaacgc cacggagtgt ccgaacaggc
      541 cctccagggc ttcatccgcc gcgatatcgg catcaaccac ctggccgaga ttgcaggact
      601 tccgggcaag ctgatcacgc cgcgccaggc gaccgagctg tttcaggagg aaaacgagaa
      661 gatcgtcgcg gaggctgtgg tctttcccgc gtcgaaccac atcgccagcg tccagttgga
      721 tcccgcggcc atcggccagt tctattcgaa tcgactgagc gtctatcgca cccctgaccg
      781 cgtccagttg cactacctgc gcttcgacgt gacgaattat ctggcgcagg ccgaggctgc
      841 cctggcccga cgcacaaacc tggccgccga aatcgagcgc atctacctgt cgagcaatcc
      901 caacgccttt gtcgacacca acggacaggt catggcaccc gaggcggcca aggaacgaat
      961 cctcaaccaa acccagcgcc agcaatccct cgcagaggcc cataaggcag ccgccctctt
     1021 tgccagtgcg tttgagacga tgaccaattt gaccgcggac accttcgtcg cccaggcggc
     1081 cgaaaagggg ctggtcacgg ccctcaccca gcccttcgcc gagaccgagt ccccgcaagg
     1141 gcttcgagtg cgcgagaatt tcgtcagcat ggcttttaag ctgacacccg aataccccgt
     1201 ctcactctct cccatccgcg gcgaagacgc ggtttatctg attgcgctca agcaacgcat
     1261 cccgagccaa atccccgcgt tggaagacat tcggacgcgc gtcgaacagg agtacatcca
     1321 ggaccaggcc gtgcggctgg cgcgcgaggc ctgcacgaat ttcgtgattc aactgaccaa
     1381 tggattggcg caaggcaaga cctgggccgc catccacgcc gaggcgggtt tgactccaat
     1441 cgccctgccc aagttcggag tcgcctcggg cccggtgccc aattgggacc ggcaggtcga
     1501 tctgaaccag gcgcgccggg cggtgcgcac gctcgatgcc ggcaggccaa ccgacgcaat
     1561 cagcacacgc gatggcggta tggtccttta tgtcaaggaa cgggtgccag tcaccgacgc
     1621 tgaactcaag gaagagctgc ccggatacct ggccaaactg cggcaaacct cggaatacca
     1681 ggccttcaac gactggttcc gcaaacaggt cgaacaaagt cgcatcgata ccggcttggc
     1741 caaagaggag acggattagc cgtctcgcat gaccagtcca atcaagctct catcgcggtc
     1801 cacccacgag tactgggaaa tcccggtcct cttcgaagac gattccctgc tggcgctcga
     1861 caaaccggcc ggactcctca cctcgcccga tcggaatgaa ccggcccgcc ccaacctgat
     1921 ggccctgctt catgcgggca ttcgagcagg cgctccatgg gcaagaacgc gcggtctcgc
     1981 ctacctcgct catgcggatc ggctcgatgc cgacacctct ggcgtgctcc tcctcgccaa
     2041 gagcaagcag attctgatca cactggccaa tcaccttggc agcgggacgc cggccaagac
     2101 cttcgtcgcc ctggtccagc gcgccccagc caaagccgct ttcgatatca tcgccaagct
     2161 ggctcccgat ccccgccagc cgggacgaat gcgcgtggac gcccaacgag gcaagaaatc
     2221 gatcacggcc tgcctggtcg aggaatcgtt tgtggggtac accctcttgc ggtgctcgcc
     2281 gcacaccaac cgcagccatc aaatccgcgc gcatctgtgg tcgcaacgcc tgcccattgt
     2341 cggcgattca ctctacggcg gccagccgct gtggctctcg cgcctcaagc gcgcctatcg
     2401 cttgaaacca ggccgtcagg agcgtccact catgggacgc gtggcgctgc atgcggcaag
     2461 cctcaatctc cctcatccgg tgaccgggga gaacatcgag attgtctccc catggcccaa
     2521 ggacctccgt gtggccgtga agtacctgcg ccagttcgcg cccgcggcct gaaacctgcc
     2581 caagccattc cggtccgagt ctgacatcga gaaccgcaca ggcgccgggt caggcctgcc
     2641 tgccggcagg caggcgaccc ggcccgcgga gcagacatcg gcgttgatcg gccggcgtgc
     2701 ccacaggcgg cctgccctcc gagattgtgc aggagaaacg cgactttgcc cgtctgcggt
     2761 cccaaccgct cgcggagaga caagttgctg ctttttctct cgattcgccc ggttcgcctc
     2821 tgctaaaagc ggactgttga gtcaataact caagaaagtg aaactaatga aacttccaaa
     2881 actcaaaatc acctgggccg ctctgttgac ggcggtggcc gttgtgtccg cccgcgcgga
     2941 ttaccccggc accgtccttt cacaggggcc cgccgcgtat taccgactga acgaaaccac
     3001 ggctccgccg cccctcacgc ttcaggcggt gaacatcggc agcgtcggcg ccgccggcaa
     3061 cggcgagttc cgcaacggcg tcgagcgggc tgtggccggc gccattgtca gcgatccgtc
     3121 gaacctcgcc gtttatatgc cgggcgccga aggcaaccgc gtccgcatcc catggaacga
     3181 agcctggaac tcggagactg ccatcagcgt cgagttctgg gccaaaccgg gccagaccaa
     3241 cgagctcgcg tgcccggcgg cctcggtcga cttcatcacg ggcccaccgg cgcagcgcaa
     3301 cggctggctc ttctaccagg gcgacaccac cctcactggc ggcaacggct ggctcttccg
     3361 ctattacaac aacagcagcc tgaccgccgt gagcggcgcg gccgtcacga tggatttgag
     3421 cactgaccag tggtaccacg tcgtcggggt ctacgacggg acgaatatca aaatctacgt
     3481 gaa
//
LOCUS       DAOQRO010000355         3474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_30265_length_3474_cov_3.754607, whole genome shotgun sequence.
ACCESSION   DAOQRO010000355 DAOQRO010000000
VERSION     DAOQRO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30265_length_3474_cov_3.754607"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            773..3343
                     /locus_tag="PKK20_11665"
     CDS             773..3343
                     /locus_tag="PKK20_11665"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV00586.1"
                     /translation="MPIGFWLLAFCLQPSALAQDSICARVKIEILQELTLEREAFEAR
                     MTINNGVTGMPIDNIGVDVNFADKDRQPVRASSNPNDTSALFFIRLQDGYSIPSSIAG
                     GTSGTIKWLIIPAPGAAGSSAQGELYYVGATLTYRAGGTDNEVEVDPDDILVKPMPAL
                     TLDYFLPSEVYGDDPFTENFVEPPIPFSLGVRVKNSGLGAARKLKIDSAQPRIVENKQ
                     GLLVDFKLHGCEVNGGAAQPTLLADFGDIAPNRSGVARWIMTSSLSGRFVEFTAYFTH
                     AAELGGQLTSLITAQPKTHFLVQDVLVDLPGRDAVRDFLVKDGFELRVYESENADTAV
                     NDQSATSSLVTDSGRYRVRTAPIPGFLFIKLIDPLAGRQPLRLAQRADGKLLPGPNAW
                     LSQTWDRNALRWDYYVNLFDVNNTAGADYLLQYKDAPARTNRPPVLDPISNWAIGPND
                     RLTFPITAIDPDGDRLAYQLESPAPAGASVTPEGTFAWQPGLAPIPSTNRMTVTVTDN
                     GLPALSDRRGFTVIVRDPTASEPPVLVLSSNRLVYPEQSGPIQLDPMARVTDEDTTVF
                     DQGRLTVAVIEGGTEKDALALAEPSATQTNITVTAENVVEYREVPVGTLTVFSGETNV
                     LTVDFNAKATIEVVEEVVRVVTFQNTEIRGASPERVIQLSLSDGQGGDSAPVDLAVEI
                     VPANRPPVAVDDEAATTADMPIALAIAKLVGNDSDPDGDALAFELLGSITAQRGIVEV
                     QGNAVLYFPDRLLVGMDSFNYRVTDPYGGWAEATVFIEVRAATTTTPSMVWIKPGPDG
                     TVEVNMMGIPNRTYTAQWSEDLLYWAYLRSVTATPTGQVQFIDQTTDQFWRFYRIVYP
                     "
BASE COUNT          663 a         1031 c         1178 g          602 t
ORIGIN      
        1 cgggtgaagg tcaaagaccc ctcaccccag ccctctccca tcggatggga gaggggggcg
       61 ctcggacgcg ctcgaagaac gggacgcttg cgggcgggcg gaggatgtcg gtcgtgtccc
      121 atctcccgtt ggccctgcct gccgaagcct gcgtttatgg atcggtggaa cctcggaggc
      181 cggcgcaggc aggcgggaga gggtcagggt gagggagccg tgcgaactcc tgcacgagcc
      241 tgaggacgat gtcgagtgaa cagtgagatg acatgggact gcacgggtga aggtcaaaga
      301 cccctcaccc cagccctctc ccatcggatg ggagagggag gacgctcgga cgcgctcgga
      361 gaacggggcg cttgcgggcg ggcggagggt gtcggtcgtg tcccctctcc cgttggacgg
      421 gagagggtca gagtgagggt gcagtgcgga cacctctacg agcctgagga cgatgatcag
      481 tgaacaatga aatgacatgg ggctgccggg tgaaggtcaa agacccctca ccccagccct
      541 ctcccacctg cctccgccaa ggcttcggca ggcaggtccg attggagagg gaggacgctc
      601 ggacacgctc ggcggttggc tggcgggtgg gaaacggagc gaacgtactg gaagatggag
      661 gcacacgttt atgaagagcc tgggatcatt acgagcagag gaccgtggag gttgccggtc
      721 agcgcgagac ttccgctctc gaggtttgtt ctaccgacta ccgatccagc tcttgccgat
      781 cggcttttgg ctcctcgcct tctgccttca gccttcagcc ttggcccaag actcgatctg
      841 cgcccgggtg aagatcgaga ttctccagga attgacgctc gaacgcgagg cgttcgaggc
      901 gcgaatgaca atcaacaacg gagtcaccgg catgccgatc gacaacatcg gtgtggacgt
      961 caacttcgcc gacaaggatc ggcaacccgt gagagcaagt tccaatccga atgacacgtc
     1021 ggccttgttc ttcatccggt tgcaggatgg ctacagtatt cccagctcga tcgcgggagg
     1081 cacctccggc acgatcaagt ggctgatcat cccggcgccg ggcgcagcgg gtagcagcgc
     1141 gcaaggtgag ttgtactatg tgggggcgac gctcacgtac cgcgccgggg gtacggacaa
     1201 cgaggtcgaa gtggacccgg acgacatcct ggtgaaacca atgccggccc tgacgctgga
     1261 ctactttctg ccaagcgaag tgtacggcga cgacccgttc acggagaact tcgtcgaacc
     1321 gcccatcccc ttctcgctag gggtgcgggt gaagaactcg gggctcggcg cggcgcgcaa
     1381 gctcaagatc gactccgccc agccgcgcat cgtcgagaac aagcagggct tactcgtgga
     1441 cttcaagctg cacgggtgcg aggtgaatgg cggggcggcg cagccgacgt tgctggccga
     1501 tttcggcgac atcgcgccga atcggtcggg cgtggcccgc tggatcatga ccagttcgtt
     1561 gtcggggcgc tttgtcgagt tcacggcgta cttcacccac gccgctgagt tgggcggaca
     1621 gttgacctcg ctgatcaccg cccagccgaa gacgcatttc ctcgtgcaag atgtgctggt
     1681 ggacctgccg gggcgagacg cggtccggga tttcctggtc aaagacggtt tcgagctgcg
     1741 ggtgtacgaa tccgagaacg ccgacacggc ggtgaacgac cagtccgcaa cctcgagcct
     1801 cgtgaccgac agtgggcggt accgggttcg caccgcgccc ataccgggct tcttgttcat
     1861 caagctgatc gatccgctgg cgggccggca gcccttgcgc ctggcccaac gcgctgacgg
     1921 gaagctgttg cccggcccaa acgcatggct gtcgcagacc tgggaccgca acgccttacg
     1981 ctgggattac tacgtcaacc tcttcgatgt gaacaacacc gcgggcgccg attacctgct
     2041 gcaatacaaa gacgcaccgg cccggaccaa tcggccgccg gttctcgatc cgatctcgaa
     2101 ttgggccatc gggccgaacg atcgtctgac gttcccgatc acagccatcg acccggacgg
     2161 cgaccggttg gcgtaccaac tcgagtcgcc cgcgccagcc ggcgcgtcgg tgacaccgga
     2221 ggggacgttc gcctggcagc ccggcctcgc gccgataccc agcacaaacc ggatgaccgt
     2281 gacggtgacc gacaacgggc tgccggccct gagcgaccgg cgggggttca ccgtgatcgt
     2341 gcgcgacccc acggccagcg agccgcctgt gctggttctc tccagcaatc ggctcgttta
     2401 tccagagcag agcgggccca tccaactcga ccccatggcg cgggtaacgg acgaagacac
     2461 gacggtattt gaccagggac gattgacagt ggcggtgatt gaaggcggga cggagaaaga
     2521 cgcgctggca ttggccgaac cgagtgccac ccaaaccaac atcacggtca ccgcggagaa
     2581 cgttgtcgaa taccgcgagg ttccggtggg taccctgacg gttttcagcg gcgagaccaa
     2641 cgtgttgacg gtggatttca atgcgaaggc gactatcgag gtggtcgagg aagtggtgcg
     2701 ggtggtgacg ttccagaaca cagaaatccg tggtgcctct cccgaacggg tcattcagtt
     2761 gagcctcagc gatgggcagg gcggcgacag tgcaccggtc gatttggcgg tcgagattgt
     2821 ccccgcgaac cgtccgcccg ttgcggttga cgatgaggcg gcgacgaccg ccgatatgcc
     2881 gattgcactg gcgatcgcca agttggttgg gaacgactcg gacccagacg gcgacgcgct
     2941 cgcgttcgag ctgctgggga gcatcaccgc gcaacggggc atagtcgagg tccagggaaa
     3001 cgcggtgttg tacttcccgg accggttgct cgtgggtatg gattcgttca actatcgggt
     3061 cacggatcct tatggcggct gggcggaggc gacggtgttc atcgaggtcc gtgcggcaac
     3121 gacaaccacg ccgagcatgg tctggatcaa accgggtccg gacgggacgg tggaggttaa
     3181 catgatgggg attccgaacc ggacctacac agcccagtgg tctgaggacc tgctgtactg
     3241 ggcgtacttg cgatctgtga cggccacgcc gaccggccag gtgcagttta tcgaccagac
     3301 gacggaccaa ttctggcggt tctatcggat cgtctatccg tgaagacgta tgagttcgaa
     3361 tcgcttggcg gcgcgccctc accccggccc tctcctgcct gccggagcct tagtttatgg
     3421 agggcgcgtg ttcgcaggcc ggcgcaggca ggcgagagag ggtaagggtg aggg
//
LOCUS       DAOQRO010000356         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_30999_length_3419_cov_4.145957, whole genome shotgun sequence.
ACCESSION   DAOQRO010000356 DAOQRO010000000
VERSION     DAOQRO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30999_length_3419_cov_4.145957"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKK20_11670"
     CDS             complement(<1..810)
                     /locus_tag="PKK20_11670"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV00587.1"
                     /translation="MNRPDLVACLERRMPEALEFLRRLVETNSYTGNRDGVNRVGAIT
                     AEMFAPLGFTTEHVASTNPVWGDHLILSRPGEGADAIAMVSHLDTVFPPEEEIQNRFH
                     WQREGNRIYGPGTHDIKGGTVMMWLVLMALREHAPNLFERVNWKLFWNSSEETLSHDF
                     GVVCLERIESNTRAALVFEAEGRRERVSRLVVARKGRATWRLRVSGRGAHAGGSHPHG
                     ANAIVQMAHSIQQVAALTDYARHITFNPGCVSGGGGLNRVPHEAVAEGEFRA"
     gene            complement(807..1853)
                     /locus_tag="PKK20_11675"
     CDS             complement(807..1853)
                     /locus_tag="PKK20_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNV00588.1"
                     /translation="MSVCAWPLFVQAASIDASTTDAPLLRFHARDASEAAAWQSEARA
                     RLFALMMGGNRPDRVRLQPVVLRELELADSASRLQEIRLQTLDDRTVHAWIAVPRQPT
                     VKVGGVLALHGHGGTGEKIVRGEGLYWYGRTLVEMGYVVVAPDIGQHDLQHTNWSLMG
                     ERVWDALCCVDYLVTRPEVDTNRLAVAGLSLGGETTMYVAALDERLRLACSSGWLTTV
                     DNMKRGHCPCWNFPGLEEHFDFADIFALVAPRLLVVELGEQERAPGGFPVQIGRGVFE
                     RIQSAYEVFGAKPAVVFTVHEGGHVFVGRDFWPLVRRELGMPRTPMPDPGAPSGQGGS
                     RDAAKPQSLDSGHQ"
     gene            complement(2107..2640)
                     /locus_tag="PKK20_11680"
     CDS             complement(2107..2640)
                     /locus_tag="PKK20_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HNV00589.1"
                     /translation="MKRLLVAVAVLLVMGCVRAEVFEVDLWNGLLVGQQEVPANTSAA
                     QGGEAGLGIRYNSESNELMINVAYGMFGWNRLEGNYTGSGIYAGQIGENGDLVLDLKP
                     IQVSLNPRYGFFQGTLVLPQQYELALYQGGLYMNIKSAMYPNGEIRGQLVIPEPGTMA
                     LLGLGLGGLFFIRRVRA"
     gene            3101..>3419
                     /locus_tag="PKK20_11685"
     CDS             3101..>3419
                     /locus_tag="PKK20_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV00590.1"
                     /translation="MDGKPTLLIVDDEKPTREGLRAALEDRFEVYLAEDVATATDLLE
                     RERFDVVLTDYRLPREDGLKLVDRAKRLTRPPICILMTAYGSEELVSRAFSENRIDDY
                     IRKG"
BASE COUNT          674 a         1110 c         1013 g          622 t
ORIGIN      
        1 cgcgcggaac tcgccctcgg ccacggcctc atgcggcacg cgattcagac cgcctccacc
       61 cgaaacacaa ccggggttga aggtgatgtg tcgcgcataa tcggtcaggg ccgcgacttg
      121 ctggatcgag tgggccattt gaacgatcgc gttggctcca tgcggatggc tgcccccggc
      181 gtgcgcgccc ctcccgctga cccgcagacg ccaggtcgcc cgccctttcc gggccacaac
      241 gagcctcgag acgcgttccc ggcgcccctc ggcctcgaac accagagccg cgcgtgtgtt
      301 gctctcgatc cgctccaggc acacaacgcc aaaatcgtgc gaaagggtct cttccgacga
      361 gttccagaac agcttccagt tcacccgttc gaacagattc ggcgcatgct cgcgcagagc
      421 catcagcaca agccacatca tcaccgtgcc ccctttgatg tcgtgcgtgc ccggcccgta
      481 aatgcgattc ccttcccgtt gccaatggaa ccggttctga atctcttcct ccggcggaaa
      541 gacggtgtcc aggtgcgaga ccatcgcgat cgcatctgcg ccctcgcctg gtctcgacag
      601 aatcaggtga tccccccaga ccggattcgt cgacgccaca tgttcggtcg tgaatccaag
      661 gggcgcaaac atctcagccg tgatcgcgcc gacccggttg accccgtcgc ggttgccggt
      721 gtaactgttg gtctcgacca agcggcgcag gaactcgagc gcctccggca ttcgccgttc
      781 gaggcaggcg acaagatcag gtcggttcat tggtggcctg aatcgagact ctgcggtttg
      841 gcggcatccc gactcccccc ctggcccgac ggcgcgccgg ggtcgggcat cggggtccga
      901 ggcatcccca actcgcggcg aaccaatggc cagaaatccc ggccgacaaa cacgtgcccg
      961 ccctcgtgca ccgtgaacac cacggcgggc ttggccccga agacctcgta ggcgctttgg
     1021 atgcgctcga agaccccgcg accgatctgg acagggaacc cgcccggcgc gcgctcctgc
     1081 tcgccaagct cgaccacaag aaggcgcgga gcgaccaacg caaagatgtc ggcgaaatcg
     1141 aaatgctctt cgagcccggg gaagttccag cacgggcagt ggccgcgctt catgttgtcc
     1201 accgtggtga gccaaccgct gctgcaggcc agccgcaagc gctcatcgag cgcggccacg
     1261 tacatggtgg tctcgccgcc caacgaaagt ccggccacgg ccaggcggtt cgtatccacc
     1321 tcagggcgcg tcacgagata gtcgacgcaa cagagcgcat cccagacgcg ctcgcccatg
     1381 agactccagt tcgtgtgctg gaggtcgtgc tgcccgatgt ccggcgccac caccacgtag
     1441 cccatctcga ccagggtccg tccgtaccag taaagtccct cgccgcggac gattttctcg
     1501 ccggtgccgc cgtgcccgtg caatgccagg acgccgccga ccttcactgt aggctgccgc
     1561 ggaacggcga tccacgcatg cacagtgcga tcatccagag tctgcagcct gatctcctgc
     1621 aaccgggatg cgctgtcggc aagttcgagc tcgcgcagga cgactggctg caatcgcact
     1681 cgatccgggc gattcccgcc catcatcagg gcgaacaagc gcgcgcgagc ctcgctctgc
     1741 catgcggcgg cctcgctagc gtctcgcgca tggaaacgca gcaatggagc gtcggttgtc
     1801 gaggcgtcga tggatgccgc ttggacgaaa agcggccacg cgcagacgct catcgagcca
     1861 agcgcgaatg ccagcttgac tgcacgaagc cgaccgagag gcccgccctc gtgctcttgt
     1921 cgtcttcgcg gcgagtttgg atcaggcatg gggagagcat tgacattcat aggcttgatg
     1981 cgcatgggga acatgaatcc gttcaggggg cagccctccg ggagaggcgg ttcgtgggag
     2041 gcacaacaca aggggcgcag ccactgctgc gcccctctgt cttttggaaa atctgaaatc
     2101 cgaggattac gcgcggactc tgcggataaa gaaaagaccg cccagcccga gtcccaacaa
     2161 ggccatcgtg cccggctcgg gtatgaccaa ctgaccgcga atctcgccgt ttgggtacat
     2221 ggcgctcttg atgttcatgt acaacccgcc ctggtacaaa gccagttcat actgttgcgg
     2281 tagaacgaga gtgccttgga aaaagccgta cctcgggttg agactgacct gaatcggctt
     2341 cagatccaaa accagatcgc cgttctcgcc gatttgcccc gcgtaaatgc ccgacccagt
     2401 gtaattcccc tcgagccgat tccagccaaa catcccgtac gcgacgttga tcatgagttc
     2461 gttggattcg ctgttgtagc gaattccgag accggcctcg ccgccctggg cggcgctggt
     2521 gttggccggg acctcttgct gccccacgag gagaccgttc caaaggtcca cttcaaatac
     2581 ctccgccctc acgcacccca tcactaagag aaccgccacc gccacgagca gtcgcttcat
     2641 atgaacttcc ctttcagcct gagttagatt agacgttgtc tggttagtca cgcgggccgt
     2701 ttctgtcaag agaaagttgg tgatattcag cgcgaccgag ttgtggtaag gaggggttgt
     2761 ggtacggccg ctttgaccga aaggcaccca aggcgaggtt gcgtcttcca aggcattaca
     2821 tgctgctggc gaacaacttg caagtctgca cccccctgag tgcctcgatc gggcaaacca
     2881 ccatccagcc aacagcgtac acggggaaca tgcctttgct ctaccatgac cctcgacacg
     2941 ttttcacgct gccttgggcg tagcgcggat tctcaatctg ccgtacagtt tctcgcctcg
     3001 gccatccgcg cgaagaatcc gaaatgcgcc ccagaatgcg aaggagcgaa atttgctgtg
     3061 gtcagtccgg cacggctgtg gttgaaatag acggacggat atggacggca aacctacgct
     3121 gctgattgtc gacgatgaga agccgacgcg cgaagggctt cgcgccgcac tcgaggatcg
     3181 tttcgaggtt tatctggcgg aggatgtcgc tactgcgacc gacctgttgg agcgggagcg
     3241 attcgacgtg gttctgaccg attaccggct accgcgggaa gacggtttga agctggtgga
     3301 tcgagcgaaa cggctcacgc gcccgcccat ctgcatactc atgacggcgt acgggtccga
     3361 ggaattggtg agccgcgcct tcagcgagaa ccggatcgac gattacatcc gcaagggaa
//
LOCUS       DAOQRO010000357         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31314_length_3396_cov_4.853038, whole genome shotgun sequence.
ACCESSION   DAOQRO010000357 DAOQRO010000000
VERSION     DAOQRO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31314_length_3396_cov_4.853038"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..3235)
                     /gene="secA"
                     /locus_tag="PKK20_11690"
     CDS             complement(131..3235)
                     /gene="secA"
                     /locus_tag="PKK20_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNV00591.1"
                     /translation="MLGFIIKKVIGSKNDREVRRLSRTVVPRINEIEQSLQSLPDEAL
                     REKTAAWKARLAAIEDKQEIQSALDEILPEAFAVVKNACRRLLGKDITVRGHVIKWDM
                     VPFDVQLIGGWALHKGRITEMATGEGKTLVATLPVYLNALAGRGVHLVTVNDYLAARD
                     SEWMGAVYRFLGLTVGCILHDQPPALRREQYNCDITYGTNAEFGFDYLRDNGIYITKE
                     EQVQRGHHYAIVDEVDSILIDEARTPLIISGPAVVSAGIELYMQFKGQVESIVRAQER
                     LCNRFLTEAQDLLKKLRPEDGSNPVDPEDLQRQIGLLLYRVKMGNPRSPNLHALLENP
                     ENAQMMNAAELELHADQKKVLLYGEKEQLLYAIDEKGHEADLTEKGRAFLSPQDPDAF
                     VVPDLITAFHEVDTGPEPDVRKRVEMKAKLQKEFEAKAQRIHCISQLLKAYGLYQRDV
                     QYVVQDNKVVIVDENTGRLMTGRRWSDGLHQAVEAKEGVRVEEENQTLATITIQNYFR
                     MYQKLAGMTGTAETEASEFWDIYKLGVLVIPPNKPCVRKDAHDTVYKTKREKYNAAVS
                     EIQSIHAQGRPILVGTISVEVSEHLSRMLKKVGIVHSVLNAKYHQQEAEIVSRAGQHG
                     SVTIATNMAGRGTDIKLGSGVADLGGLHVMGTERHEARRIDRQLRGRCARQGDPGSSH
                     FYISLEDDLMRLFGSDKIVKVMERVGLEEGQELEHPFLNRSIEQAQKRVEQHNFQIRK
                     RTLEYDDVMNKQREVVYGFRNEIIHGADTRDRLMDIMEEAVIDKVKQFTSADTEPDTW
                     NKRGLADWVNLSFPIGLPEDDILKIADTGQDVPVPGSLFDGLNAGQYAVARHIGKAVR
                     DLYELKISFENPDNLRSVERYTILSAIDKLWQEHLYHMDSLRNSIGLRAYGQRDPLIE
                     YKVEAFKMFDELMLNIKTEICRNIFLSASSLNAFEQFFRNLPQRTVHSQISAFGPAPA
                     TAAAGAAAPRSGHGGKRASDMVTEATEEIARARTPVRAMPKVGRNDPCPCGSGKKYKH
                     CCGR"
BASE COUNT          540 a          971 c         1141 g          744 t
ORIGIN      
        1 gtggcaacgg aggcaaccct cgaaggctcg tggtctcacg gaatggccgg atttgggcat
       61 gcgcgagtcc gggcgatcgc attgcgccaa agcattcaac agaccacgcg cagccttgcg
      121 aaatggcggt tcagcgtccg cagcagtgct tgtacttctt gccgctgccg caggggcagg
      181 ggtcgttgcg accgaccttg ggcatggcgc ggacgggggt gcgggcgcgg gcgatttcct
      241 cggtggcttc agtgaccatg tcgctggcgc gcttgccgcc gtggccggag cggggagcgg
      301 ctgcgcccgc ggcggcggtt gcgggggctg ggccgaaggc gctgatctgg ctatggacgg
      361 tgcgctgggg caggttgcgg aagaactgtt cgaaggcgtt gaggctcgag gcgctgagga
      421 agatattgcg gcagatctcg gtcttgatgt tgagcatgag ctcgtcgaac atcttgaagg
      481 cttccacttt gtattcgatg agcgggtccc gctggccata ggcgcgcaat ccgatgctgt
      541 tgcgcaggct gtccatgtgg tagagatgct cctgccacag tttgtcgatg gcgctgagga
      601 tggtgtatcg ctcgaccgag cgcagattgt cggggttttc gaagctgatc ttgagttcgt
      661 agaggtcgcg gaccgccttg ccgatatgcc gggccacggc gtattgaccg gcgttgagtc
      721 cgtcgaacag cgatccgggc acggggacat cctggcccgt gtcggcgatc ttcaggatgt
      781 cgtcctcggg cagtccgatg gggaaactca ggttgaccca atccgccaaa ccgcgcttgt
      841 tccatgtgtc cggctcggtg tcggcgctcg tgaactgttt gaccttgtcg atgaccgcct
      901 cctccatgat gtccatgagg cggtcgcgcg tgtcggcgcc gtggatgatc tcgttgcgaa
      961 agccgtagac gacctcgcgc tgtttgttca tgacgtcgtc gtactcgagg gtgcgtttgc
     1021 gaatctggaa gttgtgctgt tccacccgct tttgtgcctg ctcgatcgag cggttcagaa
     1081 acgggtgttc gagttcctgg ccttcttcga gaccgacacg ttccatgacc ttgacgatct
     1141 tgtcggagcc gaagagccgc atgagatcgt cttcgaggga gatgtagaag tgggacgaac
     1201 cggggtcgcc ctgtcgggcg catcgtccgc ggagttggcg atcgattcgg cgggcttcgt
     1261 ggcgctcggt gcccatgaca tgcaggccgc ccagatcggc aacgccggac cccagtttga
     1321 tgtcggtgcc gcgtccggcc atgttggtgg cgatggtgac tgagccgtgc tggccggcgc
     1381 ggctgacgat ttcggcctcc tgctggtggt acttggcgtt gaggaccgag tgcacgatgc
     1441 cgaccttctt gagcattctc gatagatgct cgctgacctc gaccgagatg gtgccgacca
     1501 ggatggggcg gccttgggcg tggatggact ggatctcgct gaccgcggcg ttgtacttct
     1561 cgcgtttcgt cttgtagacc gtgtcgtggg cgtctttgcg gacgcacggt ttgttgggtg
     1621 gaatgaccag cacgcccagt ttgtagatgt cccagaactc agaagcctcc gtctccgccg
     1681 tgccggtcat cccagccagc ttttggtaca tgcggaagta gttctggata gtaatggtgg
     1741 cgagggtttg gttttcttcc tcgacgcgca cgccctcctt ggcctcgacg gcctggtgca
     1801 gaccgtcgct ccagcggcgg cccgtcatga ggcgtcccgt gttttcgtcg acgataacga
     1861 ccttgttgtc ctggacgacg tattggacat cgcgctggta gaggccgtag gccttcagga
     1921 gttgggagat gcagtgaatc cgctgggcct tggcctcgaa ttccttctgc agcttggcct
     1981 tcatctcgac gcgcttccgc acatccggct ccggcccggt gtcgacctcg tggaacgcgg
     2041 tgatcaggtc cgggacgacg aaggcgtctg gatcctgcgg gctgaggaag gcgcggcctt
     2101 tctcggtcaa atccgcctcg tggccttttt catcgatggc gtagaggagt tgctcctttt
     2161 ctccgtagag cagcaccttc ttctgatcgg catgcaactc gagttcggcc gcgttcatca
     2221 tctgggcgtt ctcggggttc tcgagtagcg catgcaggtt gggcgagcgc gggttgccca
     2281 tcttgacgcg gtagagcagc agcccgattt ggcgctgaag atcctcgggg tcgacgggat
     2341 tggagccgtc ttcggggcgc agtttcttga ggagatcctg ggcctcggtg aggaagcggt
     2401 tgcagagccg ttcctgggca cggacgatcg actcgacctg ccctttgaac tgcatataca
     2461 actcgattcc cgcgctgacc acggcgggcc cgctgatgat gagcggcgtg cgcgcctcat
     2521 cgatcaggat ggagtcgacc tcgtcgacga tcgcgtaatg gtgcccgcgt tgaacctgtt
     2581 cttctttggt gatgtagatg ccgttgtcgc gcaggtaatc gaagccgaac tcggcgttgg
     2641 tgccgtaggt gatgtcgcag ttgtactgct cgcgtcgcaa cgcgggcggc tgatcgtgca
     2701 ggatgcagcc gaccgtcagg cccaggaacc ggtacaccgc ccccatccac tcgctgtcgc
     2761 gcgcggcgag gtagtcgttg acggtcacga gatgcacgcc gcgcccggca agcgcgttga
     2821 gataaactgg cagggtggcc accagcgtct tgccttcgcc cgtggccatc tcggtgatcc
     2881 gccccttgtg cagagcccat cctccgatca actgcacgtc gaaggggacc atgtcccact
     2941 tgatgacatg cccccgcacg gtgatgtctt tgcccaacag ccggcgacag gcattcttga
     3001 ccaccgcaaa ggcctccggg agaatctcat cgagcgcgga ctggatctcc tgtttgtcct
     3061 cgatggcggc gaggcgcgcc ttccacgcgg cggttttctc gcgcagggcc tcgtcgggca
     3121 gcgactgcag ggactgctcg atctcgttaa tgcgcggcac gaccgtgcga ctcagacgcc
     3181 tcacctcgcg atcgttcttc gatccgatga ctttcttgat gatgaaccca agcatgtgta
     3241 caaaacggtt tggagaacct acgggaaggg tgggagagcg tcaaaaggat tcaggacgca
     3301 ctgtgcgtct gaaaacggag cgagttcctg gcccgagtca gccaggggcg cgccgggcac
     3361 ctccaggaaa cgggtcagcg gctcccagcg tttgag
//
LOCUS       DAOQRO010000358         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31331_length_3395_cov_4.013772, whole genome shotgun sequence.
ACCESSION   DAOQRO010000358 DAOQRO010000000
VERSION     DAOQRO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31331_length_3395_cov_4.013772"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="PKK20_11695"
     CDS             complement(<1..2193)
                     /locus_tag="PKK20_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNV00592.1"
                     /translation="MRLHLLIATTAVLCSSHATGRSAASPASDVNLAVVATPTCSYVS
                     GDTRTSALNDGVSPRSSRDDRRGTYGNWPRQGTQWVQYDWTQPISTARIEVYWWDDAR
                     GVRLPKASRVKYWDGKDFIPVPNPTGLDIEANRFNVTTFDEITTSKLRLEMDGDGEFS
                     TGIIEWRVLDSGRSPRFPPRVAAGVDRTTVLGGQTYLSGQVQSLKPADTPLTLTWTKQ
                     AGPGRVTFANPNAADTAAGFDQPGDYVLALTADEGGASASATLKVRVEPPPPTHRLDV
                     VHTKRYKIDSPLWNSRARSLIVNWIPHCIDYINRTNLTQGQGGIDNFVEAAKALRGEA
                     HAPHKGYVFANAWVHQTVESICIALMVDPQGDYDVIEAQQAMRATLEDWIPKILAAQE
                     PDGYLQTAYTLADRSAWPERWSPRNRGDHEGYVAGYFIESAINHHTLTEGKDLRLYDA
                     AKRLADCWVANIGPGKKDWYDGHQEMEQALVRFGRFVNDVEGNGRGDAYIKLAKFLLD
                     CRNNGSEYDQSHLPVQRQYEAVGHAVRAVYSYSGMADVAAETADVDYQSAVLSLWDNM
                     VNRKYYVTGGIGSGETSEGFGPDYSLPHSAYCESCSSCGLIFFQHKLNLAYHDARYAD
                     LYEETIYNALLGAVDLEGKTFNYTNPLIGGRRTPWHACPCCVGNIPRTLLMIPTWTYL
                     KTADAIHVNLFIGGTITLERVAGTDVEIVQKTDYPWDGKVALTVNPREP"
     gene            2302..>3395
                     /locus_tag="PKK20_11700"
     CDS             2302..>3395
                     /locus_tag="PKK20_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNV00593.1"
                     /translation="MTSQVPRAELDDRMQRFRSRMEAICPDWELAAFLGRVNQYYLTG
                     TMQDGLLLVPRDGDAVFWVRRSLERACAESLFPDMRPMRSFRDAAQGMKPARGRRTIH
                     IEADLVPYGLVERFRKHFPCDELGSLEPVIGRVRSVKSPYELEIIERAGAIHRRAMED
                     KVPGLLREGMSEAEFACDLYRLMVSEGHEGTVRFAMFNVDIAVGQLGFGENSLYPTSF
                     NGPGGCRGVGPAAPVLGSRDRRLRVGDLVFVDLGCSVEGYATDKTMIYAFGRPLPREA
                     SASHARCVEIQGRIAASLKPGAIPSTIYSTVMESLDPGFLEHFMGFGSRRAEFLGHGV
                     GLQIDEPPVIAEGFDEPLIEGMVLAVEPKRG"
BASE COUNT          572 a          916 c         1214 g          693 t
ORIGIN      
        1 gggttccctg gggttgacgg tgagggcgac cttgccgtcc cagggataat cggtcttctg
       61 aacgatctcg acatcggtgc ctgccacgcg ttcgagggtg atggtgccgc cgatgaatag
      121 gttgacatgg atggcgtcgg cggtcttgag gtaggtccag gttgggatca tcaggagcgt
      181 gcgggggata ttgccgacgc aacaggggca ggcgtgccag ggcgtacggc gtccaccgat
      241 gagcgggttg gtgtaattga aggttttgcc ttcgaggtcc acggcgccga ggagggcgtt
      301 gtagatcgtc tcctcgtaga gatcggcgta cctggcgtcg tggtaggcga ggttgagctt
      361 gtgttggaag aagatcaggc cgcaactcga acaggactcg caataggcgc tgtggggcag
      421 cgagtagtcg gggccgaaac cttccgaagt ctcgccgctg ccgatgccgc cggtgacgta
      481 gtacttccgg ttcaccatgt tgtcccagag cgagagcacg gcgctctggt aatcgacatc
      541 ggccgtttcg gcggccacat ccgccatgcc cgagtagctg tagacggcgc gcacggcatg
      601 gccgacggct tcgtattggc gttggacggg gagatggctc tggtcgtatt cgctgccgtt
      661 gttgcggcaa tcgagcagga atttggcgag ttttatgtaa gcgtcgccgc gtccattgcc
      721 ctcgacgtca ttgacgaagc gtccgaagcg cacgagggcc tgttccattt cctgatggcc
      781 gtcgtaccag tccttcttgc ccggcccaat gtttgccacc cagcagtcgg ccaaccgttt
      841 ggcggcgtcg tagagacgga ggtccttgcc ctcggtgagt gtgtggtgat tgatggcgga
      901 ctcgatgaag tagccggcga cgtaaccctc gtgatctccg cgattgcggg ggctccagcg
      961 ttccggccag gcactgcggt ccgccagggt gtaggcagtt tgcaggtaac cgtcgggctc
     1021 ttgcgccgcg agaatcttgg gaatccaatc ctcgagggta gcgcgcatgg cctgttgcgc
     1081 ctcgatgacg tcgtaatccc cttgcggatc gaccatgagg gcgatgcaga tcgattccac
     1141 cgtctgatgc acccatgcgt tggcgaagac gtagcctttg tggggggcgt gggcctcgcc
     1201 gcgcaaggcc ttggcggcct cgacgaaatt gtcgatgccg ccctggccct gggtgaggtt
     1261 cgtgcggttg atgtaatcga tacaatgggg aatccaattg acgatgagcg atctggccct
     1321 cgagttccag agggggctgt cgatcttgta gcgcttggta tggacgacgt ccaatcggtg
     1381 cgttggcggc ggcggttcga ccctgacctt gagagtggcg gaggcgctcg ctccgccctc
     1441 gtcggcggtg agtgcgagaa cgtaatcgcc gggctgatcg aatccggcgg cggtgtcggc
     1501 ggcgttggga ttcgcgaagg tcacgcggcc gggtccggct tgcttggtcc aggtcagcgt
     1561 gagcggggtg tcagccggct tcagcgactg gacctgtccg ctcaggtagg tttggccgcc
     1621 gagaaccgtc gtccgatcca cacccgccgc cacccggggc ggaaaacgcg gcgaccgtcc
     1681 ggaatcgagg acgcgccatt cgatgatgcc tgtcgagaac tcgccgtcgc cgtccatctc
     1741 gagccgcagc ttcgatgtgg tgatttcgtc gaatgtggtg acgttgaacc ggttggcttc
     1801 gatgtcgagg ccggtgggat tcgggacggg tatgaagtcc ttcccatccc agtatttcac
     1861 tcgcgaggct ttgggcaggc gcacgcccct ggcgtcgtcc caccaataga cttcgatccg
     1921 cgcggtgctg atgggctggg tccaatcgta ttgcacccat tgtgttccct gacgcggcca
     1981 gttgccatac gtaccgcgac gatcgtcgcg tgaactgcgt gggctgacgc catcgttcag
     2041 ggccgacgtg cgggtgtcgc ccgagacata ggaacaggtg ggtgtcgcaa caacggcgag
     2101 gttgacgtcg gatgcgggcg aagcggcgga tcgcccggtc gcatggctcg aacaaagaac
     2161 cgcggtcgtc gcaatcagga gatggagtcg catgggagga ctgaatcaat tggccggagc
     2221 gatgtccagt gaagattgat tgtggcagca cttcctcctt ttcgcggacg ggcgggggcg
     2281 cataatggcg gcagccaagt catgacatca caggtgcctc gcgctgaatt ggacgaccgg
     2341 atgcagcggt ttcggtctcg gatggaggcg atttgccccg actgggaact ggcggcgttt
     2401 ttgggacgcg tcaaccagta ctacctgacc ggcacgatgc aggacggctt attgctggtc
     2461 ccgcgcgatg gagacgcggt gttctgggtg cgtcggagcc tggaacgtgc ctgtgcggaa
     2521 tcgctgtttc cggacatgcg cccgatgcgg agtttccggg atgcggcaca aggaatgaag
     2581 ccggcgcgag ggcggcgcac cattcacatc gaggccgatc tggtgccgta cggacttgtg
     2641 gaacgctttc ggaagcactt tccatgcgat gaactcggct cgctcgagcc ggtgattggg
     2701 cgggtgcgat ccgtcaagag cccgtacgaa ctggagatca tcgagcgggc cggggcgatt
     2761 caccgtcggg ctatggaaga caaggtgccg gggttgctgc gcgagggcat gagcgaggcc
     2821 gagttcgcct gcgatttgta caggctcatg gtgagcgaag ggcatgaggg cacagtgcgc
     2881 ttcgcgatgt tcaatgtgga cattgcggtg gggcagctcg ggttcgggga aaattcgctc
     2941 tatcccacca gttttaatgg cccgggtgga tgccgcggag tggggcccgc cgcgccggtg
     3001 ctcggaagcc gcgaccggcg cttgagggtg ggcgatcttg tgtttgtcga tctgggctgc
     3061 agcgtcgagg gatatgcgac ggacaagacg atgatctatg cgttcgggcg cccgctgcct
     3121 cgagaggcaa gcgcctctca cgcgcggtgc gtcgaaatcc agggccgcat cgctgcgtca
     3181 ctgaagcccg gggcgatccc atccacgatc tacagcactg tgatggagag tctggacccc
     3241 gggttcctcg agcatttcat ggggttcggg agtcgtcggg cggagtttct cggacatgga
     3301 gtagggttgc agatcgacga gccgccggtg attgcggagg ggttcgatga gccattgatc
     3361 gaggggatgg tgctggcggt cgaaccgaag cgagg
//
LOCUS       DAOQRO010000359         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31477_length_3384_cov_5.003004, whole genome shotgun sequence.
ACCESSION   DAOQRO010000359 DAOQRO010000000
VERSION     DAOQRO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31477_length_3384_cov_5.003004"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKK20_11705"
     CDS             <1..667
                     /locus_tag="PKK20_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV00594.1"
                     /translation="AGRGRVRTHLRSESSLPKVFDFVREKLGEGRQAYIVCPRIEEGD
                     GPAAVKTVREEFDRVRTLFAPHAVGLLHGRLPPQEKDRVMEGFRAGRIAVLVATSIIE
                     VGVDVPNATVMVIENAESYGLAQLHQLRGRIGRGREESHCILIAAACTDEARRRLEAL
                     EQTNDGFRIAETDLLLRGPGEFLGQAQSGMPRFRFVELARDLALVEQARTAAAGLLDS
                     DAA"
     gene            704..1066
                     /locus_tag="PKK20_11710"
     CDS             704..1066
                     /locus_tag="PKK20_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00595.1"
                     /translation="MKTMFSALLIALFVLTACGRKDSSSASGQTDAGAGGNPLTAPAD
                     YLGAIGQAQKHSLKTVDLASVTKAIQMFRTEEDRFPSDLKELVKEGYLPALPQLPAGM
                     AYQYDANTGQVKAVRQQP"
     gene            1090..2151
                     /locus_tag="PKK20_11715"
     CDS             1090..2151
                     /locus_tag="PKK20_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00596.1"
                     /translation="MTYPDLVAIACLALAHGLAFAAPPDQGPFEIPVLVISFFPTNGT
                     RIDIRETGDWDAPLDETRSKAQRLTREVMSALEQGSRYHGYKNPTAQPSLRYSVAGEL
                     EFLRPLPTVAKTGHTTPMTDYARIMADVDLREWVEKRGVREVWIWGYHGGKVDLWESN
                     MASPYGDVSNSDRDPHDLPVFGRTYTVYHYNYQRGASEAVENHMHQIEAVLNAVDGRD
                     RTPPERWPDLLFWGRFVGSDRSHKIVRPGCGWAHYPPNGEQDYDWANPRFVLTDIEDW
                     KPDGTGHKQKMNCERWGGDSLRWFVYWMQNLPGKDNGLSDHGKPLRNWWVFLGDYDAA
                     KRHGMTLADEPDSIERAPK"
     gene            2151..3113
                     /locus_tag="PKK20_11720"
     CDS             2151..3113
                     /locus_tag="PKK20_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNV00597.1"
                     /translation="MSVDEPSASGLATVALARAAMHTRFELLLHGADPVALRAAGEQA
                     LDEIERLEEQLSLYRPTSEIAQVNRRAFERPVPVSPNVFRLLECAAELSRATGGAFDP
                     TIAPLVRCWGFMGGKGCRPEDDAIDVARGQVGIDGIELDSAKFTVRFRRPGMMLDLGA
                     IGKGCAIDAAVDLLREAGIESAFIHGGTSSSYGLGFPPDASAWKAAIPVPPDLTRTGT
                     VCIRRGVPPSGDSTNLLGEVALTDLSLGVSGIWSKSFREGDRTFGHVIDPRLGEPVQR
                     ACLAAVVLPCATETDALSTALLVLGVEGLDVISRLRPNARFLLA"
BASE COUNT          608 a         1024 c         1140 g          612 t
ORIGIN      
        1 tgccggtcga gggcgggtgc gcacgcatct ccgctccgaa tcgagtttgc cgaaggtgtt
       61 cgattttgtc cgtgagaagc tcggcgaagg gcggcaggcc tacatcgtct gcccgcggat
      121 cgaggagggg gacggcccgg cggccgtgaa gacggtgcgc gaggagttcg atcgggtccg
      181 gacgctgttt gcgccgcatg cggtggggct gctgcacggg cgattgcccc cgcaggagaa
      241 ggaccgtgtg atggaagggt tccgggccgg gcgcattgcg gtcctggtcg cgacctcgat
      301 catcgaggtg ggtgtcgatg tgcccaacgc cacggtcatg gtgatcgaaa atgcggagag
      361 ctacggtctg gcccagcttc accaactgcg cgggcggatc ggacgaggcc gcgaggaatc
      421 gcactgcata cttattgccg cagcctgcac ggacgaggcg cgcaggcggc tcgaagcgct
      481 cgagcagacg aacgacggat tccggattgc agagactgac ctgctgctcc gggggccggg
      541 tgagtttctg ggccaggcgc agagcgggat gccgcgtttt cgttttgtgg aactggcgcg
      601 ggacctggcg ttggtggaac aggctcgaac ggcggctgcc ggccttctcg attcggatgc
      661 ggcttagtct tggatctcgg cgcggatttg gtagtgtcgg ctcatgaaaa cgatgttcag
      721 cgccctgctg attgccctgt tcgtcctgac cgcctgcggg cgcaaggact catcgtccgc
      781 gtccggccag accgacgccg gcgccggcgg caacccgctg accgcccctg ccgattacct
      841 gggcgcaatc ggtcaagcgc aaaagcactc gctgaagacg gtggacctcg catcggtgac
      901 caaggcgatt cagatgttcc ggaccgagga ggaccgtttt cctagcgatc tgaaggaatt
      961 ggtcaaggaa gggtatcttc cggcgctgcc acaactgccg gcaggcatgg cctatcagta
     1021 cgatgccaac accggccagg tcaaagccgt gcggcaacag ccctgaacat gccgaatccg
     1081 gccacgatca tgacgtatcc agatcttgtg gccattgcct gcctcgccct cgcgcacggc
     1141 ctcgccttcg ccgccccgcc ggatcagggg ccattcgaga ttcccgtgct ggtgatcagc
     1201 ttcttcccga ccaacgggac gcggatcgat atccgggaga cgggcgactg ggacgcgccg
     1261 ctcgacgaga ctcgatccaa ggcgcagcgt ttgacgcgcg aggtgatgag cgcgctcgaa
     1321 caggggtcgc gctaccacgg atacaagaat cccacggctc aaccgagcct gcgttattca
     1381 gtggcgggcg aactcgagtt cctgcgcccg ctcccgacgg tcgccaagac cgggcacacg
     1441 acgcctatga ccgattacgc gcgcatcatg gccgatgtcg acctgcgcga gtgggtggag
     1501 aaacggggcg tgcgcgaggt gtggatatgg gggtaccacg gcggcaaggt ggatttgtgg
     1561 gaatcgaaca tggcgagtcc ttatggcgac gtgagcaaca gcgaccgcga tccacacgac
     1621 ctgccggtgt tcggacgcac ttacaccgtg taccactaca actatcagcg cggcgcttcc
     1681 gaggcggtcg aaaaccacat gcaccagatc gaagccgtgc tcaacgccgt cgatgggcgc
     1741 gaccgcacgc ccccggagcg ctggcccgat ttgctcttct gggggcgttt tgtggggagc
     1801 gatcgctcgc acaagatcgt gcgccccgga tgcggctggg cgcactatcc gcccaatggg
     1861 gagcaggact atgattgggc caatccgcga ttcgtcttga ccgacatcga ggattggaag
     1921 cctgacggca cggggcacaa gcagaagatg aactgcgagc gctggggcgg cgacagcctc
     1981 aggtggtttg tctattggat gcagaatctg cccgggaagg acaacggcct tagcgaccat
     2041 ggaaaaccct tgcgcaactg gtgggtcttt ctcggcgact acgatgcggc gaaacgccac
     2101 ggcatgacgc tggccgacga acccgacagc atcgagcgcg ctccaaagtg atgagcgtcg
     2161 atgagccatc cgcatccggt ttggcgactg tcgcgctcgc gcgggcggcg atgcacaccc
     2221 gtttcgagct tctcctgcat ggcgccgatc ccgttgcgct ccgcgcggca ggcgagcagg
     2281 ctttggatga aatcgagcgg ctcgaagagc aactcagcct ctaccgcccc acgagcgaga
     2341 ttgcccaggt caatcgccgc gcgttcgaac gtcccgtgcc tgtcagcccg aatgtcttcc
     2401 gcctgctcga atgcgccgcc gagttgagtc gcgcgaccgg gggcgctttc gatcccacca
     2461 tcgcgcccct cgtgcggtgc tggggtttca tgggcggaaa ggggtgtcgg cccgaggatg
     2521 acgcgattga cgttgcgcga gggcaagtcg ggatcgacgg gatcgagttg gatagcgcga
     2581 agttcaccgt gcgatttcgg cgtccgggga tgatgcttga tctgggggcg attggcaaag
     2641 gctgcgccat tgacgcggcg gtcgatctgc tgcgcgaggc gggcatcgag agcgccttca
     2701 tccacggcgg caccagcagc agctatggcc tcgggttccc cccggacgca tccgcctgga
     2761 aggccgccat ccccgtaccg cccgatttga ctcgaaccgg caccgtgtgc atacggcgcg
     2821 gcgttccccc gtcgggggac agcacgaatc tgctcggcga agtcgcgctc accgatctct
     2881 cactcggcgt ctcgggcatc tggagcaagt ccttccgtga aggggatcgt accttcgggc
     2941 acgtgattga tccacggttg ggcgaaccgg tccagcgagc gtgtcttgcg gccgtcgtcc
     3001 tcccttgcgc caccgaaaca gacgccctct cgactgcctt gctggtcctg ggcgtcgagg
     3061 gcttggacgt catcagccgg ctccggccca acgctcgatt cctgctggca tgaaaaggga
     3121 aaagaggtca gtgctcacaa tcgacaatca agagtccaat agcaggcaat aaagccagca
     3181 tcggagcgga acacggagtg ggttgattct tgattgcatt cggcatgggt gatcgtaagt
     3241 gggcgctcgc atgggcgaac gaggtcagcg acgggagcca gccgccgatg aagttcgatt
     3301 tgtctctgag cgaatcggct ggctcccgtt cgctggaccg tctggttcgc ctattccagt
     3361 ttctgcttgg ctgggcggac ttca
//
LOCUS       DAOQRO010000360         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31565_length_3379_cov_4.925090, whole genome shotgun sequence.
ACCESSION   DAOQRO010000360 DAOQRO010000000
VERSION     DAOQRO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31565_length_3379_cov_4.925090"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /gene="secF"
                     /locus_tag="PKK20_11725"
     CDS             <1..638
                     /gene="secF"
                     /locus_tag="PKK20_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNV00598.1"
                     /translation="ESFPQAGMTVVGTDSVGPSVGAQIQRSAIIASLLAMFGILVYVA
                     FRYEFSFAVASVLALLHDVLLTMSIYFLSGRQLTAPMVAAILTIIGYSINDKIVIMDR
                     IREDLRLGIRGSFRELIDIALNQTLSRTIITGTAVLLATLALYLFGGNVINDLAFAFL
                     IGILSGTYSSLMIACPIVLWWHKGQRPKIGAEAVAIDQTVVAAPQGRVKPA"
     gene            690..1664
                     /locus_tag="PKK20_11730"
     CDS             690..1664
                     /locus_tag="PKK20_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNV00599.1"
                     /translation="MLAVIEIQWWHWVSFILVIIFFLALDLGVFHRKAHVVGFREALV
                     WTSVWFCMAMTFALTLVPVRGRGEATDFLQGYLIELSLSMDNVFVIAVIFRYFRVPSL
                     YQHRVLFWGILGALIMRGLMIWLGAELIHSYEWVLYIFGLFLVLTGLKMLTTDDTEEV
                     RPERNPVLRLVRRFFPFTTEYDGQRFFTRHHGHPALTPLALVLIMVETTDLVFALDSI
                     PAIFGVTTVPFIVFTSNAFAILGLRSLYFVLAGAVTYFRYLKVGLAVVLVFIGFKMLI
                     VDWVKIPSRWSLTVVAGVILGSILVSIIAERRDRAAKRLSEPAPPQDS"
     gene            1888..>3379
                     /gene="recJ"
                     /locus_tag="PKK20_11735"
     CDS             1888..>3379
                     /gene="recJ"
                     /locus_tag="PKK20_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNV00600.1"
                     /translation="MRHRWLLAAAQPERAHSIAAALGASPLLAQCLINRGQPDADQAS
                     AFLTPRLRHLADPNHLPNMPAAVDRLWRARQSDEPVVIFGDYDVDGVTATALLLEVLQ
                     PLGWRAVSYLPHRLDEGYGLTDEAVDRCLERSPARLLLAVDCGSTTCDTIARLKTRGV
                     DVIVLDHHQVSSPPPPAVALVNPQLAPPGPDNLQQLCSVGLAFKLAHALVKRGRALGC
                     PLAEQFDLRPLLDLVALGTIADMVPLTGENRVLASAGLARINALQRPGLLALKDVARV
                     PGAIGAWEVGFQIAPRLNAAGRLENAKAALDLLLARDPASAEAGARLLDAQNRERQQI
                     ERAIAEEVVAAVRARFQPDTDRVIVEGNLLWHVGVVGIVAARVLNEFYRPTLILGGDG
                     YAWRGSGRSIEGFDLAAALRACDDLLLRHGGHAMAAGLTIRPENLPALRERLNRLARQ
                     ALDLHDLSPPLRLDAVTPLRELTLERVDELRRLEPRGYTALHGGVSA"
BASE COUNT          517 a         1245 c          969 g          648 t
ORIGIN      
        1 aagagtcctt cccacaggcc ggaatgaccg ttgtggggac agacagcgtc gggccgagcg
       61 tcggcgccca gatccagcgc tccgccatca tcgcctcgct gctggccatg ttcggcattc
      121 tcgtgtacgt ggcattccgc tacgaattct cgttcgcggt cgcgtcggtg ctcgcgctct
      181 tgcacgacgt gttgctcacg atgagcatct acttcctgag cggacgccag ctcacggcgc
      241 cgatggtggc ggccatcctg accatcatcg gctattcgat caacgacaag atcgtgatca
      301 tggaccgcat ccgcgaggac ctcagactgg gaatccgcgg ttcgttccgc gagctgatcg
      361 acatcgccct caaccagacg ctgagccgga cgatcatcac gggcaccgcg gtgctgttgg
      421 cgaccctggc cctctacctg ttcgggggca acgtgatcaa cgacctcgcc ttcgccttcc
      481 tgatcggcat tctgagcggc acctattcga gtctgatgat tgcctgcccg atcgtgctct
      541 ggtggcacaa agggcagcgt cccaagatcg gcgctgaggc cgtggccatc gaccagaccg
      601 tggttgcggc gccgcaaggc cgggtcaagc cggcctgacg gcggccgcgg tttgtgcatg
      661 ccttcgaagg ctccagcctg accgcagtga tgctcgcagt catcgaaatc caatggtggc
      721 attgggtcag cttcatcctg gtcatcatct tctttctcgc cctcgacctc ggggttttcc
      781 accgcaaggc ccatgtcgtc ggattccggg aggcgctggt ctggacgtcc gtctggttct
      841 gcatggccat gaccttcgcc ctgactttgg tgcccgtgcg cggacgtggc gaagccaccg
      901 actttctcca gggctacctg atcgaactgt cgctctcgat ggacaacgtg ttcgtcatcg
      961 ccgtcatctt ccgctacttc cgcgtcccca gcctgtacca gcaccgcgtt cttttctggg
     1021 gcattcttgg cgcgctgatc atgcgcgggc tgatgatctg gctgggcgcc gaactgatcc
     1081 acagctacga atgggtcctc tacatcttcg gccttttcct tgtcctcacc ggcctcaaga
     1141 tgctcacgac cgatgacacc gaggaggtcc gccccgaacg caatcccgtc cttcgcctgg
     1201 tccgccgctt cttccccttc accaccgaat acgatgggca gcgcttcttt acccgccacc
     1261 acggtcaccc cgccctgacg ccgctggcgc tggtgctcat catggtggag acgacggacc
     1321 tggtgtttgc cctcgattcg atccccgcca tcttcggcgt caccaccgtc ccgttcatcg
     1381 tcttcacgtc caatgcgttc gccatcctcg ggctgcgctc cctctacttc gtcctggccg
     1441 gcgccgtcac ctatttccgc tatctcaagg tcggtctcgc cgtcgtcctc gtgttcatcg
     1501 gcttcaaaat gctcatcgtt gactgggtca aaatcccaag tcgatggtcc ctgaccgtcg
     1561 tcgcaggcgt tatcctcggc tccatactcg tctcgatcat cgccgagcgg cgggaccgcg
     1621 ccgcaaaacg cctgtccgag cctgcgccgc cacaggactc atgagttctg gcgaggcccg
     1681 cctcggacca agtcaaaacc ctcaacacca tgaaccatcc ccgctgcttc cccgccctcc
     1741 tctcccgcgc ctcgctcgtt ggaatccttg ccaaaaagag gggatagggg acatggcaga
     1801 acttgatttg attgcactgc gttctgagct tcgaggattc caccttatgc ccgccgcgcc
     1861 ctccccagaa tcgcccggcc accggttatg aggcatcgct ggctcctggc ggcggctcag
     1921 cctgaacgcg cccattcgat tgccgcggcg ctcggtgcct ccccacttct ggcccagtgt
     1981 ctcatcaacc gcggccaacc cgacgcagac caggccagcg cattcctgac cccccgcctc
     2041 cggcatctgg ccgatccgaa ccatctgccc aacatgcccg ccgccgtcga tcgcctctgg
     2101 cgagccaggc agagcgacga gcccgtggtc atcttcggcg attacgatgt cgatggcgtc
     2161 accgcgacgg cgctcctgct cgaagtgctg cagccgctgg gctggcgcgc ggtctcctac
     2221 ctgcctcatc ggctcgatga gggatacggc ctcacagacg aagcagtcga ccgctgcctg
     2281 gaacgttccc ccgcacggct cctgctcgcc gtcgactgcg gttccaccac ctgcgacacc
     2341 atcgcccgcc tcaagacacg cggcgtcgat gtcatcgtcc tcgaccacca ccaggtctcg
     2401 tctccgccgc cgcccgccgt ggcgctcgtc aatccccaac tcgccccgcc cgggcccgac
     2461 aaccttcaac aactctgctc ggtcggcctc gcgttcaagc tggcccatgc cctggtcaag
     2521 cgcgggcgcg ccctgggctg cccgctcgcc gaacagttcg acttgcggcc cctcctggat
     2581 ctggtcgccc tcggcacgat cgcggacatg gtgccgctca caggcgagaa ccgggtgctt
     2641 gcctcggccg gccttgcccg catcaacgcc ctgcaacggc ccggcctcct cgctctcaaa
     2701 gatgtggcgc gcgtgccggg cgcgatcggc gcatgggagg tcggattcca gatcgcgccc
     2761 cgattgaacg ccgcgggccg gctcgaaaac gccaaggcgg ccctggacct cctgctcgcc
     2821 cgcgatccgg cgagcgcgga ggccggtgcc cgcctcctgg acgcccagaa tcgcgagcgc
     2881 caacaaatcg aacgtgccat cgccgaggag gtggtggcgg ccgtccgcgc ccggttccag
     2941 ccggataccg accgcgtcat cgtcgagggt aatctgctct ggcacgtcgg cgtcgtcggc
     3001 atcgtcgccg cgcgcgtgct caatgaattc tatcgcccga ccctgatcct gggtggcgac
     3061 ggctatgcgt ggcgcggatc cggacgcagc atcgaggggt tcgacctggc agcggctttg
     3121 cgggcctgtg acgacctact cctgcggcat ggcgggcacg ccatggccgc cggactcacc
     3181 atccggcccg agaacctccc cgccctgcgg gaacgactca accgcctcgc ccgccaggcg
     3241 ctcgacctcc acgatctgag cccgccactg cgactggatg ccgtcacgcc gctgcgcgaa
     3301 ctcaccctcg aacgcgtgga cgaactccgg cgactcgagc cgcgcggtta cacggccttg
     3361 cacggcggcg tcagcgccg
//
LOCUS       DAOQRO010000361         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31610_length_3376_cov_4.962060, whole genome shotgun sequence.
ACCESSION   DAOQRO010000361 DAOQRO010000000
VERSION     DAOQRO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31610_length_3376_cov_4.962060"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..>3376
                     /locus_tag="PKK20_11740"
     CDS             121..>3376
                     /locus_tag="PKK20_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00601.1"
                     /translation="MKCLSILGCLAMGAVIALGANAPAYGILREVWEGIPGNDLAALT
                     NAPAYPAQPTSTNYVTDLFEAPTDVLDQYGQRMHGYVVPPRTGAYTFWIASDDNGALF
                     LSSDESPARARLIASVNSWTSSREWEKEANQRSSPVNLIAGRPYYIAALMKEGGGGDN
                     LAVRWLMPDGTDQAPIVATNLLPYGISFTPPVIASHPTNTTVIEGDLARFSITLSTVG
                     LYTYRWQRNGIAIAGADEPELVYGPVRLGDHQVRFRCVVTNALGSAVSNEAVLSVLPD
                     TTPPTLAGVRNLGLTRLEVAFSEPVAHSTALDPANYSLDGGLVVTAVNATSDPRVVEL
                     VTSAMTYGHIHTLTVNRVTDLAATPNVIAPDSQASYTVNEFVPTDVGSPALAGSTVSV
                     PGGVDVTGAGKTIGGTADQFQFGWQERTGNFDFQTRIEAVTVSDPFLHAGLMARETLD
                     ANARFAGVFAGSHQLGCFFESRTTVGGNSTTATPRGGFPVNHPSTWLRLQRTGTALQG
                     YASLDGRTWTPLGSATLSGLAPKVYLGFAVTSDSEQTLATARFRDSGVPESTATGAPA
                     LNKEPIGPSSRATGMVISEIMYHPPPRDDGRNLEFVELYNARSVFEDLTGWRLSGEIS
                     YRFPPGFRLQAGETVVIAAAPDDIRAVYGITNVLGPYTGALANEGGKIRLRNNADAIR
                     LEAVYSDASSWPAADGAGHSLVLARPSYGEADPRAWAASEFRGGSPGGPDPTMPAPET
                     SIVINEFLAHTDEPMLDFIELYNRSNDRVDLSHMVLTDRPHALRFRIPEGTWIEPRGF
                     LAWDQTQLGFALNSSGETVYLINAAGTRVLDAIRFGAQENGVSSGRSADGAPTIRRLA
                     QPTPGAANAPWRIEDVVINELMYNPISGDNNDEFIEIYNRLTTPLDLTGWRFVQGVNY
                     TFPAGVTLPANGYLVVARNAARLRTNYPHLNNANCVGDFQGSLSNRGERIALAKPDLV
                     VTTNATGRVETNVAHIVVSEVTYADGGRWGQWADGGGSSLELIDPRADLLRPSNWADS
                     DETSKAPWTTVETTGRIDNSSGSANRLHVASLGGGECLVDDIEVFRAGSTNVVNN"
BASE COUNT          600 a         1261 c          969 g          546 t
ORIGIN      
        1 cgaaacgaca tcgggacacg cgttggcttg cgacttgctg ctgccgtctg gtatgtttgc
       61 gccgtacacg atgaacagca acacttcatc cgcctgcgcg gcagacgggc tttcaatgcc
      121 gtgaaatgtc tctcgatcct cggttgtctg gccatgggcg cggtgatcgc gctgggcgcc
      181 aacgcgcctg cctatggcat cttgcgcgaa gtctgggagg gcatcccggg aaacgatctg
      241 gccgcgttga ccaatgcgcc cgcctacccc gctcaaccca ccagcaccaa ttacgtgacc
      301 gatctttttg aggcgccgac cgacgttctc gatcagtacg gccagcgcat gcatggctac
      361 gtcgttccgc cccgcaccgg cgcctatacg ttctggatcg ccagcgacga caacggcgcg
      421 ctctttctca gctcggacga gagcccggcc agagcccggc tcatcgcctc agtcaactcg
      481 tggacgtcct ctcgcgagtg ggagaaagaa gccaaccagc gatcgagccc tgtcaatctg
      541 atcgccggca gaccgtatta catcgctgcc ctgatgaaag agggcggcgg cggagataat
      601 ctcgccgttc gctggctgat gcctgacgga accgatcagg cgcccatcgt cgccacgaac
      661 ctcttgccct acggcatcag cttcaccccg cctgtcattg cctcacaccc caccaacacc
      721 accgtcatcg agggcgacct cgcccgcttc agcatcaccc tctcgaccgt cggactctac
      781 acctaccgct ggcagcgcaa cgggattgcc atcgccggtg ccgacgaacc cgagttggtc
      841 tacggaccgg tgcgccttgg cgatcaccag gtccgcttcc gttgcgttgt caccaacgcg
      901 ttgggcagtg ccgtcagcaa cgaggcggtg ctgtctgtcc tgcccgacac caccccgccg
      961 acgctggcgg gggtgcgcaa tctcgggttg acgcggctgg aagtcgcctt cagcgagccg
     1021 gtggcgcatt cgactgccct cgaccccgcc aactacagcc tggacggggg cctggtcgtc
     1081 acggcggtga acgccacctc tgacccacga gtggtcgaac tcgtcacttc cgcgatgacc
     1141 tacggccaca tccacaccct cacggtcaac cgcgtgaccg acctcgctgc aacccccaac
     1201 gtcatcgccc ccgactcaca ggcgtcctac accgtcaatg aattcgttcc gacggatgtc
     1261 ggatcccccg cgctggccgg ctccaccgtc tccgtccccg gcggcgttga cgtcaccggc
     1321 gccggcaaga ccatcggcgg cacggcggat cagttccagt tcggctggca agagcggacg
     1381 ggcaacttcg acttccaaac ccggatcgag gccgtgacgg tcagcgaccc attcctgcac
     1441 gccggcttga tggcgcgcga gaccctcgac gccaacgcgc gttttgccgg cgtcttcgcc
     1501 ggttcacatc aactgggctg tttcttcgaa tcacggacga ccgttggcgg caacagcacg
     1561 accgctaccc cccgcggcgg attcccggtc aatcacccct ccacctggct ccgcctccag
     1621 cgcaccggca ccgccctcca gggctacgcc agcctcgacg gccgcacatg gaccccgctc
     1681 gggtccgcga cgctgagcgg cctggccccg aaggtctatc tcggattcgc cgtcaccagc
     1741 gacagcgaac aaacgctggc gaccgcccgt ttccgcgact ccggcgtccc cgaaagcacc
     1801 gccaccggcg ccccggccct gaacaaggag cccatcggcc cctcgagccg cgccacgggc
     1861 atggtcatca gcgagatcat gtaccatccc ccaccgcggg acgacggacg caacctcgag
     1921 ttcgtcgaac tctacaatgc ccggtccgtc ttcgaagatc tcaccggctg gcgtctgtcc
     1981 ggcgagatca gctaccggtt cccacccgga ttccgtctcc aggcgggcga gacggtggtc
     2041 atcgccgcgg cgccggacga catccgggcc gtgtacggca tcaccaacgt gctcggtccc
     2101 tacaccggcg cgctcgccaa cgaaggcgga aagattcggc tgcgcaacaa cgctgatgcc
     2161 atccggctcg aagccgtcta ctccgatgcg tcatcctggc ccgcggcgga cggcgcgggt
     2221 cattcgctgg tgttggcccg gcccagctac ggcgaggccg acccgcgcgc ctgggcggcc
     2281 agcgaattcc ggggcggatc gcccggcgga cccgatccga cgatgcccgc ccccgagacc
     2341 agcatcgtca tcaacgagtt cctcgcccac accgacgagc ccatgctgga tttcatcgaa
     2401 ctctacaatc gctcgaacga tcgcgtcgac ctctcgcaca tggtgctgac cgaccggccc
     2461 cacgcacttc gcttccgcat ccccgaaggc acctggatcg aaccccgggg ctttctggcc
     2521 tgggaccaga cccagctcgg ctttgccttg aactcgagcg gcgagaccgt gtacctcatc
     2581 aacgccgccg gcacccgtgt gctcgatgcg atccgcttcg gcgcacagga aaacggtgtc
     2641 tcgagcggac gctccgccga cggcgccccg acaatccggc gcctggcaca gcccaccccc
     2701 ggcgccgcca acgcgccctg gcgcatcgag gacgtcgtca tcaacgagtt gatgtacaac
     2761 ccgatctccg gcgacaacaa cgacgagttc atcgagatct acaatcggct cacaacacca
     2821 ctcgacctga ctggctggcg cttcgtccag ggcgtcaatt acacgttccc cgccggcgtc
     2881 acccttccgg caaacggcta cctcgtcgtc gcccgcaacg ccgcgcgact ccggacgaat
     2941 tacccgcacc tgaacaacgc caactgtgtc ggcgacttcc agggctcgct cagcaaccgc
     3001 ggcgaacgca tcgccctcgc caagcccgac ttggtcgtga ccacaaatgc caccggccgc
     3061 gtcgagacca atgtggcgca tatcgttgtg tccgaggtca cgtacgccga cggcgggcgt
     3121 tggggtcagt gggccgacgg cggcggctcg agtctcgaac tgatcgatcc gcgcgcggat
     3181 ctcctgcgtc cctcgaactg ggccgacagc gacgagacct cgaaagcgcc ttggacaacc
     3241 gttgagacca ccggtcgcat cgacaacagc agcggcagcg ccaaccgact gcacgtcgcg
     3301 tcgctcggcg gcggcgaatg cctcgtcgac gacatcgagg tcttccgcgc cggcagcacc
     3361 aatgtcgtca acaacg
//
LOCUS       DAOQRO010000362         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31747_length_3366_cov_4.802175, whole genome shotgun sequence.
ACCESSION   DAOQRO010000362 DAOQRO010000000
VERSION     DAOQRO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31747_length_3366_cov_4.802175"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1979
                     /locus_tag="PKK20_11745"
     CDS             126..1979
                     /locus_tag="PKK20_11745"
                     /inference="COORDINATES: protein motif:HMM:NF027641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00602.1"
                     /translation="MSTRDLNLKGRRIVLLIGGLVLLAAVGSCFAAERAGNSSVLEFI
                     RAQSERRLTQVPREVLAFYYTWYGRPERHGRWVHWGNVDADRHDISESTHYPALGAYD
                     SHDPAVIDRHIDQAKAHGLTGFIATWWGQNTYDDRAFVTLLERAEKKNFKVTVYWETA
                     PATGQRQVDQAINDLAYVLQRYGSSPALLKVEGKPVVFVYGRVMGQVPPKSWPAIVQG
                     AREKAGDALLIADGYQAGYARMFDGVHTYNICDWVQGKRPDELRALSAQAFAHAVQLA
                     RTHGRISCLTVIPGYNDTKIRTPGINAERQGGLTYRVLWEEAIKADPDWVLITSWNEW
                     HEGSEIEPSWEDGDAYLKLTGEYAPRFRTNPVSKARGLSLAALAPEKARQVQAWFAGK
                     TVGLLPDFSGDAAFWLLDAGVDVRELTWADLLDSQRFNPQKLPLILHAGGEHYVRSVE
                     AEGDVEQALQRYLAAGGFLVAIPSLPFPFFYDDAAGRPAPIASKVGLPVTGGWETPPS
                     NVALTFHLNTELLPELSRTVAFPAAGDRRWRPAMREQTHPEDFYVSLARLKDADEKNY
                     GDGMAYVEHRAGARRGGKSLYVWMRMPDVLGLNECLYAVFQFAAQTLGSTE"
     gene            complement(2028..3029)
                     /locus_tag="PKK20_11750"
     CDS             complement(2028..3029)
                     /locus_tag="PKK20_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00603.1"
                     /translation="MRMPFGMWFLVAASLQIAHDACAGETFPKTRLALREGQWTINLR
                     PTHAGSAAEGLLMNVRMVNAVFEDRKRPQFDPEANTDAFIVRLPEYAAHGIDAFTLCL
                     QGGMPGYEGAVNSAFDPDGSLRPHYLTRVERVIRACDRQGLAVILGLSYQRQSAILRD
                     EAAVRAGVANAARWVRDRGFGNVVLEIANEYPHRGFVHAVIRDSKGQASLIRLARETA
                     PGLLVSASGYGDGAIDPEVAEAADFLLPHWNGTKVEQIPARIAALKRFGKPIVCNEDD
                     KTGAEAVAALRACLENGSSYGLMLKERNQFFPFRFEGAVDDPEFYAELRKVTTPQTP"
BASE COUNT          618 a         1015 c         1079 g          654 t
ORIGIN      
        1 ggtcgagtat ctcaggggat acactccctc cctcgagcct tgcatctgac ccggtggcgc
       61 tcccgcgaga atcggaagta attcttgcac agaccataac cacccgagct caccttgaaa
      121 ccacgatgag tacacgcgat ctgaatctga aagggcggcg gattgtattg ttgattggcg
      181 gactggttct gctggctgca gttggatcgt gtttcgctgc cgaaagggcg ggcaactcca
      241 gcgtgctcga gttcattcga gcgcagagcg agcggcgtct gacacaagtg ccgcgagaag
      301 tgttggcctt ctattatacg tggtatggcc ggccggaacg gcacgggcgc tgggtgcatt
      361 ggggaaacgt cgatgcggat cgacatgaca tttccgaatc gacgcactat ccggccttgg
      421 gcgcctacga ttcgcacgac cccgcggtga tcgaccggca catcgatcag gccaaggcgc
      481 acggcctgac gggcttcatc gccacgtggt ggggccagaa cacctacgac gaccgtgcgt
      541 ttgtcaccct gttggaacgg gcggagaaga agaacttcaa ggtgaccgtg tattgggaga
      601 cggcccccgc gaccggccaa cgccaggtcg accaggccat caacgacctg gcttacgtgc
      661 ttcaacgcta tgggtctagc ccggctttgc tcaaggtcga gggtaagcca gtggtttttg
      721 tctatggccg agttatgggt caagtgccgc ccaagtcctg gccggccatc gttcagggcg
      781 cccgggagaa ggccggcgat gcgttgctga tcgccgacgg ataccaggcc gggtatgccc
      841 ggatgttcga cggcgttcat acgtacaaca tctgtgactg ggtccagggc aaaaggcctg
      901 acgaactacg cgctctgagc gctcaggcgt tcgctcatgc cgtccaattg gcgcgcaccc
      961 acggccgcat cagttgtctg acggtcattc ccggttacaa cgacaccaag atacgcaccc
     1021 ccggcatcaa cgcggaacgt caggggggcc tgacctatcg tgtcctgtgg gaagaggcga
     1081 tcaaggccga tccggattgg gtgctgatca cctcgtggaa cgagtggcac gaaggctcgg
     1141 aaatcgagcc gagttgggag gatggcgatg cttacctgaa actgactggc gagtatgcgc
     1201 ctcggttccg gaccaatccc gtgagcaagg cgcgcggctt gtctctcgcg gcattggctc
     1261 cggaaaaggc ccggcaagtt caagcctggt ttgccggcaa gacggtcggc ctgctgcccg
     1321 acttctccgg cgatgcggcg ttctggctgc tggatgccgg ggtcgatgtc cgggagttga
     1381 cttgggccga tctgctggat tcgcagcggt tcaatccgca gaagctgccg ctgatcctgc
     1441 atgcgggagg cgagcactac gtgcgcagtg tcgaagctga gggtgatgtc gagcaagccc
     1501 tccaacgcta cctggctgct ggcggtttcc tggtggcgat cccgagcctg ccgttcccgt
     1561 ttttttacga tgacgctgcg ggccggcctg cgccgatagc cagcaaggtg ggccttccgg
     1621 tcaccggggg atgggaaacg ccgccgtcca acgtggcgct gacgttccat ctgaacacag
     1681 agttgttgcc agagctgtcg cgaacggtcg cttttccggc tgcgggcgat cggcgctggc
     1741 ggccggcaat gcgcgaacag acacatccag aagattttta tgtgtcgttg gcccgactca
     1801 aggacgccga cgagaagaac tatggcgatg gcatggctta cgttgaacat cgcgcgggag
     1861 cgcgacgtgg aggcaagagc ctttacgtct ggatgcgcat gccggatgtg ttgggcctaa
     1921 atgagtgcct ctacgccgtg tttcagttcg ccgcgcagac acttggatcg accgagtgag
     1981 ggaaatctgg gcgcttccgt gacgcacttg attggaagta agcgtcgtta gggagtttgc
     2041 ggcgtggtga ccttccgcaa ttccgcgtag aattccgggt cgtccaccgc accctcgaac
     2101 cggaacggaa agaactggtt gcgttccttg agcatcaggc cgtagctaga gccgttttcg
     2161 aggcaggcgc gcagggcggc gacggcctcg gcgcccgtct tgtcgtcctc gttgcacacg
     2221 atcggcttgc caaaacgctt cagcgccgcg atgcgagccg ggatttgctc gaccttcgtg
     2281 ccgttccagt gtggcaagag aaagtccgcc gcctcggcca cctccggatc aatggcgccg
     2341 tcgccgtacc cgctggcgga aaccaggagt cccggcgcgg tttcccgggc caggcggatc
     2401 aggctggcct ggcctttcga atcgcggatc actgcatgca cgaatccgcg gtgcggatac
     2461 tcattggcga tttccaacac cacattccca aacccgcggt cgcgcaccca gcgtgcggca
     2521 ttcgctacgc cggcgcgcac cgccgcctcg tcgcgcagga tggccgattg cctttggtag
     2581 gacagtccga gaatgaccgc cagaccctgc cggtcacacg cccggatgac tcgttcgacc
     2641 cgtgtcaggt agtggggccg caagctgccg tccggatcga acgccgagtt caccgcgcct
     2701 tcataccccg gcatcccgcc ttgcaggcac agggtgaaag cgtcgattcc atgagccgcg
     2761 tactccggaa gccgcacgat gaatgcatcg gtgtttgcct ccggatcgaa ctgcggcctc
     2821 ttccggtcct cgaagaccgc gttgaccatg cggacgttca tcagcagccc ctcggcggcg
     2881 ctgccggcat gggtcggacg caggttgatg gtccattgcc cctcacgcag ggcgagtcgg
     2941 gttttcggga acgtctcccc cgcgcatgcg tcgtgcgcga tctgtagaga ggcggcaacc
     3001 aggaaccaca ttccaaatgg cattctcata gacttgtcac ccgcggcaca gttcgatcag
     3061 ccatgcctcg attctgcgac gggcagaagt gctggggcac ttcgcgcagc aaccgatccc
     3121 ggcctcgcac ggcctggaga gtagtctccg ttgcgctgat ggacaaccgc cgttctggcc
     3181 gaaagagagg atcgacagga tgatcggtga tgggctccac tcagactcag gacggaacac
     3241 agatgggcac ctagacgttg atcccgctgc ggctgaccgg attcacgcag cttcgtgctc
     3301 tctgtggttt acacccctct cggtgcggtg caggcaccgt cttgggccga ggatttgggc
     3361 caccga
//
LOCUS       DAOQRO010000363         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_31826_length_3361_cov_3.366606, whole genome shotgun sequence.
ACCESSION   DAOQRO010000363 DAOQRO010000000
VERSION     DAOQRO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31826_length_3361_cov_3.366606"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKK20_11755"
     CDS             <1..507
                     /locus_tag="PKK20_11755"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNV00604.1"
                     /translation="PVSVTENYYVASKLAAGSTHEYRLAYVNQNGDRSPLSDPASGQT
                     WGDDANFDGLPDDWQAAFWGDDSASWPSPDADSDGDGASNRQEFLAGTDPTDPLKVLR
                     LNWRADGAGTSLEWNAQAGCIYQVQVSKDLNQWENIGSPRFAAGTTDAVPVDRADRKA
                     MYRVIRVR"
     gene            512..2014
                     /locus_tag="PKK20_11760"
     CDS             512..2014
                     /locus_tag="PKK20_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00605.1"
                     /translation="MMRLLRLLLVGGCLYGAWLPGRAFTLLGPPATWMTHQLGYDVSA
                     PLTTYGPMNLGEEYRWNVPILYYAFTSDFLNYFGEYGANEIDKAIAMINNLPPVSSLN
                     IDDFPAGSLRINHRAQAAGLTDLKSFALREMLYLLGIGDPTRYVFCIRNRWTPGSCPP
                     ILYHVIQRNFDPKTLRHSAYINGDLWTYTTIIDTCTPPPDYAFLAPEPVDPMALLGYR
                     HMPASSYFAAGSINGGFVTTLTRDDVAALQYIYRTNNYNVETIPAGVIGGTNYIFGAT
                     NSSPWGVPPYLLTNGYSTNLPWYPGYPWDAPPSLTNVTGTNDVTGTNSFIEPALRSGI
                     DKVLWRRLEYDSVLGNFFTPTTNVYTERVILNGRRVPQTIVRVVEVPDIIFDAADLQG
                     GEATDTVIGTAQVMTDWQNNDAINGLAGQIGPGTMVPSVGTTPAYVLTFNSVGPIWYN
                     QWPNFLSQADAIGPNQFLLWGSFDGTTNAPVVYPVGTSIEAIEAQVLSGR"
     gene            2039..>3361
                     /locus_tag="PKK20_11765"
     CDS             2039..>3361
                     /locus_tag="PKK20_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00606.1"
                     /translation="MKHLTLWALVAGMAWTTQVARAADIFINEGELNYAYGEAPQIDA
                     LAFYNAGTFTVWSAPLPYDFTRVMNYTNRGTMTGGVGFTFDNVTREGVRGPAATFVNQ
                     RGGVIRTVERFSFIASNTVYYPTYITVSASKIVNEGTLSVENSGILRLEGNKIDLTRG
                     GLSVGSVQPDFGAGYIPGGYDFRPEPGVYDLWWGVGSGPTNASGWPGQDSSSIARPLG
                     GGTSWQAVSPSHFVSDNNASGFVRVGTIATYFTGLTNVTGGIGLTITNVDGTTTNIFA
                     PTNIIRQAVFVGVSDTNNFAARARFFPSTVPDVAHATVAIELRLSNTNVVTATGETNA
                     IYFVDRLASETNLAFGTNIDITPVPTLRPYPYEVWRSPPPEFRFGFNGNALITTNFVY
                     DRGFSNRFVTNLYAGYSAYIDYLTVRPPIVAGMGPLDQPGRIEIIGNEV"
BASE COUNT          635 a         1150 c          937 g          639 t
ORIGIN      
        1 cctgtttcgg tgaccgagaa ctactatgtg gcgtcaaagc ttgccgccgg cagcacgcat
       61 gagtatcgcc tcgcctatgt caatcagaac ggagatcgtt ccccgctctc cgacccggcc
      121 agcggccaaa cctggggcga cgacgccaac ttcgacgggt tgcctgacga ttggcaggcc
      181 gcgttctggg gcgacgactc ggcctcgtgg ccgtctcccg atgccgacag cgacggcgat
      241 ggcgccagca acaggcagga attcctcgcc ggcactgatc cgacagaccc cctcaaggtg
      301 ctgcggctca attggcgcgc cgacggcgcg ggcaccagcc tcgaatggaa tgcccaggcc
      361 ggttgcatct atcaggtcca ggtctccaag gacctcaacc aatgggagaa catcggatcg
      421 ccccggttcg ccgccgggac aaccgatgca gtacctgtcg accgcgccga tcgcaaggcg
      481 atgtaccggg tgatccgagt gaggtgattt tatgatgcgt ctgcttcgac tgcttctggt
      541 tggagggtgt ctctacggcg cgtggctgcc ggggcgcgcc ttcacgctcc tcggccctcc
      601 agccacttgg atgacccatc aattgggtta cgacgtgtcc gcgccgctca cgacttacgg
      661 gccgatgaac ctgggcgagg aatatcgctg gaacgtcccg atcctctact acgccttcac
      721 ctcggacttt ctgaattact tcggggaata cggggccaac gagatcgaca aggcgatcgc
      781 gatgatcaac aacctcccgc ccgtctcgtc gctgaacatc gacgacttcc ccgcgggttc
      841 gcttcgaatc aatcaccgcg cgcaggccgc cggcctgacc gacttgaaat ccttcgcgct
      901 ccgggaaatg ctctacctgc tgggcatagg cgatccgacc cgctacgtct tctgcattcg
      961 aaaccgttgg acgcccggct cctgtccccc gatcctctat catgtcatcc agcgcaattt
     1021 cgatcccaag accctccggc acagcgccta catcaacggc gatctttgga cctacacgac
     1081 catcatcgac acctgcaccc ctccgcccga ctacgcattc ctggcgcccg aaccggtgga
     1141 cccgatggcc ctgcttggct accgccacat gccggccagt tcatacttcg cggctggcag
     1201 catcaacgga ggcttcgtga ccacgctgac ccgcgacgat gtggccgccc ttcagtacat
     1261 ttaccgcacc aacaactaca acgtcgagac catccccgcg ggagtcattg gtggcaccaa
     1321 ctacatcttc ggggcgacga attcgtctcc atggggcgtt cctccctatc tcctgacgaa
     1381 cgggtattcg accaatcttc cgtggtaccc gggctatcct tgggacgccc cccccagcct
     1441 caccaatgtg acaggcacca acgatgtgac gggaaccaac tcgttcatcg agcctgccct
     1501 gcgcagcggc atcgacaagg ttctctggcg tcggctcgaa tacgactccg tgctgggcaa
     1561 cttcttcacc ccaaccacca acgtctatac cgagcgggtc atcctcaacg gacgccgggt
     1621 accacagacc attgtgcgcg tggtcgaagt gcccgatatc atcttcgatg cggccgatct
     1681 ccagggcggc gaggcaaccg atacggtcat cgggaccgca caggtcatga ccgactggca
     1741 gaataacgac gccatcaacg gcctggccgg ccagattggc cccggaacca tggttccatc
     1801 cgtgggcacc acgccggcat atgttctcac gttcaactcg gtcgggccga tctggtacaa
     1861 tcaatggccc aatttcttga gtcaggccga tgccatcgga cccaaccagt tcctcctctg
     1921 gggttcgttt gacggaacca ccaacgcgcc tgtggtctac cccgtcggca cgtccatcga
     1981 ggcgatcgaa gctcaagttt tgagtggccg ctaggccgcg cgcacggtcc atcgcgatat
     2041 gaagcatctt acactttggg ctctggtggc ggggatggcc tggacaaccc aagtcgcccg
     2101 cgccgccgac attttcatta acgaaggcga actcaattac gcctacggcg aggccccgca
     2161 gatcgatgcc ctcgcgttct acaacgcggg caccttcaca gtctggtccg cgcctctccc
     2221 ttacgatttc acccgggtga tgaactatac gaaccggggg accatgacgg gcggcgtcgg
     2281 tttcaccttc gacaacgtca caagggaggg cgtccgcggg cctgcggcca cctttgtcaa
     2341 ccagcgcggc ggcgtcatcc ggacggtcga gcgcttcagc ttcatcgcct cgaacacggt
     2401 ctactacccg acctacatca ccgtctccgc ctccaaaatc gtcaacgaag gaacgctctc
     2461 ggtcgaaaac tccggaatcc tccgtctcga agggaacaag atcgacctga cccggggcgg
     2521 tctctcggtg ggctccgtcc aaccggattt cggggcgggc tatatccccg gcggctacga
     2581 ttttcgcccg gagcccggtg tttacgacct ttggtggggc gtcggttccg ggcccaccaa
     2641 cgcgagcgga tggcccggtc aggacagttc ctcgattgcg cggccgctgg gcgggggcac
     2701 ctcctggcag gcggtctcgc cgtcgcactt cgtgtccgat aacaacgcat cgggtttcgt
     2761 gcgcgtcggc acaatcgcaa cgtatttcac cggcctgacg aacgtgacgg ggggcatcgg
     2821 cctgacgatc accaacgtcg acggcacaac aacgaatatc ttcgccccga ccaacatcat
     2881 ccgacaggcg gtgttcgtgg gcgtgagcga cacgaacaac ttcgccgcgc gcgcccggtt
     2941 cttccccagc accgtgccgg atgtcgcgca cgcgaccgtg gccatcgagt tgcggctttc
     3001 caacacgaac gtcgtgaccg cgaccggcga gaccaatgcg atctactttg tcgatcggtt
     3061 ggcctccgaa acgaacctcg cgttcggcac gaacatcgac atcaccccgg tccccacgct
     3121 ccgtccgtac ccgtacgagg tctggcgttc gccgccgccg gagttcaggt tcggttttaa
     3181 cggcaacgcg ttgattacga cgaatttcgt ctacgaccgc ggattctcga atcgttttgt
     3241 caccaatctc tacgccggtt actcggcgta catcgactac ctgaccgtgc gcccgccgat
     3301 cgtcgcgggc atgggacccc tcgaccaacc cggacgcatc gagatcatcg gcaacgaggt
     3361 c
//
LOCUS       DAOQRO010000364         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_32433_length_3319_cov_3.725184, whole genome shotgun sequence.
ACCESSION   DAOQRO010000364 DAOQRO010000000
VERSION     DAOQRO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32433_length_3319_cov_3.725184"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKK20_11770"
     CDS             <1..297
                     /locus_tag="PKK20_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNV00607.1"
                     /translation="FAQAGSLMRKHARPDLAEPDLIELLRLDDSGFRSRFADTPLARA
                     KRRGLLRNVCVALGNIGTAVALPALECAARDHEPLIAEHASWALTRIQQRQGAH"
     gene            complement(300..>3319)
                     /locus_tag="PKK20_11775"
     CDS             complement(300..>3319)
                     /locus_tag="PKK20_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNV00608.1"
                     /translation="FISHAHRLAGCLAVGLVSTALGQHDRPAAGLPPVPVSGLAYTNA
                     FFPGATYRADVPAQESLIGFAAGERASTAAEIERCLKAWNAAAPDRTRLVEYARSHEN
                     RPLHYLLVTAPANLARLDQIQSSLARLGDPRSLSEADARGLIDTLPAVAWLAHTIHGD
                     ETEGSDAALALAYHLVAAEDSTTAQLLANLVIIIDPLMNPDGRDRFVKMIAEHRGALP
                     NLDDQSLVHEGYWPYGRGNHYLFDLNRDWALGVHPETRGRIREISRWNPQLFVDAHGM
                     GSQATHLFSPPREPVNPNIPPGRAEWGLLFAREQARAMDRIPFLYYTGEWHEEWYPGY
                     ADGWSSCRGAVGILYEQARVAEDGVRRPGGRLLSYRESVHHHIVGAMANLRTLERNAR
                     QLREYLYQTRKAALDPGGPYGQRTFAILPTANQSRLATLVDLLRLQGIELHQATAEIS
                     APRALDQLGRERQNCALPAGTLLIPNRQPLAHLVSALLEFDPHFKPEMLAEERKELLR
                     FGRSRIYDTTAWNLTMLFGLEAFTLPTDLPEGTKPFESPSKPAPILGEVDAPVAFVFD
                     GADDHAVTAAARLMERGIEVRASDKSFRLDDRDFARGSIVVTRLDNRDFSGNWRETIE
                     HTAVELGLPAVGLSTGLGEGDLPDLGGRHFRRLEPPRIGVLARGRFSSTDYGSIWQAI
                     DQHLGIRHSHLEIDSNPGDLTRYNVLIVPKTWSALPTGLLDSLKEWVRGGGTLIVVAT
                     SVNAFVAPKAEFSKARLLGDVLDRLPEYELAILREWEGWTGVVPPLDKVWRHMAKPDL
                     EYPWQTVKGAHPDEKELKKREAWQSLFMPQGAFLAARVDTDHWLTAGCDEMLPILADT
                     QPVLMAAEGVEAPVRYGHWVAARPVKYASADAGTGERRSRSDPSDPSDPQAKTTQDSD
                     KKEGKEPARLGWCAPPPGTETYLRMSGLLWPEAAHRLANSACVTRERLGRGQVILFSI
                     SPTFRGGTRGPLRIFLNAVVCGPGWGANQRIVP"
BASE COUNT          563 a         1055 c         1128 g          573 t
ORIGIN      
        1 tttgctcagg ccggatccct gatgcgcaaa cacgctcgcc ccgacctggc tgaacccgat
       61 ctcatcgagt tgctccgcct cgacgactcc ggattccgaa gccgtttcgc cgacacgccg
      121 ctcgctcgag cgaagcggcg cggccttctg cgcaatgtct gcgtcgccct tggcaacatt
      181 ggaaccgcgg ttgctctccc cgccctcgaa tgcgctgcgc gcgatcacga gcctctcatc
      241 gccgaacacg cctcctgggc cctcacgcgc atccaacaac gccagggggc gcactgaccc
      301 taaggaacga tgcgctggtt cgcaccccaa ccggggccgc agaccacagc gttgaggaag
      361 attctcagcg ggccgcgggt cccgccgcgg aaagtcggcg aaatcgagaa gagaatcacc
      421 tggccgcgcc caaggcgttc ccgggtgacg caggcggagt tggcgagtcg atgggccgcc
      481 tcgggccaga gcaagccgct catgcggaga taggtttccg tgccgggcgg tggcgcgcac
      541 cagccgagtc gggcgggctc ctttccctct tttttgtcgc tgtcttgcgt tgtcttggct
      601 tgcggatcgg acggatcgga cggatcggac cgactccgtc gctccccagt cccggcgtcg
      661 gcggacgcgt acttcacagg gcgggccgcc acccagtgac cgtaccgcac cggggcttcg
      721 acgccctcgg ctgccatcag gacgggttgg gtgtccgcca gaatcggcag catctcgtcg
      781 caaccggctg tgagccagtg gtcggtgtcg accctggcgg cgagaaaggc cccttgaggc
      841 atgaagagcg actgccaggc ctcacgtttc ttgagttcct tttcatcggg atgcgccccc
      901 ttgaccgttt gccaagggta ctcgagatcg ggcttggcca tgtgccgcca gaccttgtcc
      961 agaggcggga caacgccggt ccagccctcc cactcgcgca gaatcgccaa ttcgtactcg
     1021 ggaagcctgt cgaggacatc gcccaacagt ctggctttgc tgaactcggc tttcggggcg
     1081 acaaaggcgt tgaccgaggt cgcaacgacg atcagggttc cgcccccgcg cacccactcc
     1141 ttcagactgt cgagcagacc cgtcggcagg gccgaccacg ttttcggaac gatcaggaca
     1201 ttgtagcggg tgaggtcgcc ggggttgcta tcgatttcga ggtggctgtg gcggataccg
     1261 aggtgttggt caatcgcctg ccagatcgag ccgtagtcgg tcgaactgaa tcggccccgc
     1321 gccagcacgc caatgcgcgg cggttcgagg cggcggaagt ggcggccgcc aagatccgga
     1381 agatcgccct cgcccaggcc ggttgagagc cccacggccg ggaggcccag ttcgacggcc
     1441 gtgtgctcga tcgtctcgcg ccagttgccg ctgaagtcgc ggttgtccag tcgagtcaca
     1501 acgatcgaac cgcgggcgaa atcgcggtcg tccaagcgaa aggatttgtc ggatgcgcgg
     1561 acctcaatcc cccgctccat caggcgcgcg gcggcggtca ccgcatgatc gtcggccccg
     1621 tcgaaaacga acgcgaccgg cgcatcgacc tccccgagga tgggggcggg cttcgatggc
     1681 gattcgaatg gcttcgtgcc ttcgggcagg tcggttggga gcgtgaacgc ttcgagccca
     1741 aagagcatgg tcagattcca cgcggtggtg tcatagatgc gtgaccggcc aaaacgcaga
     1801 agctccttgc gctcctcggc gagcatctct ggtttgaagt gcggatcgaa ttcgagcagg
     1861 gcgctgacga gatgggccag cggttggcgg ttggggatca ggagcgttcc ggcaggcagc
     1921 gcgcaattct gccgttcccg ccccaactga tccagggcgc gcggcgcgct gatttcagcg
     1981 gtcgcctggt ggagttcgat gccttgaagc cggagcagat cgacgagagt cgccagccgg
     2041 ctctggttgg cggtgggcag gatggcgaac gtgcgctgac cgtatgggcc accggggtcc
     2101 agagccgcct tgcgggtttg ataaaggtac tcgcggagct gccgggcatt gcgttcgagg
     2161 gttctcaggt tggccatcgc ccccacgatg tggtggtgga cggattccct gtaactgaga
     2221 aggcgtccgc cggggcgacg caccccgtcc tccgccacgc gcgcctgctc gtagagaatt
     2281 cccacggcgc cacggcacga actccagccg tcggcatacc ccggatacca ctcctcatgc
     2341 cactcgcccg tgtagtagag gaatggaatc cgatccatgg cgcgcgcttg ttctcgggca
     2401 aagaggagtc cccactccgc ccggcccggg gggatgttgg ggttgacggg ctcgcgcggg
     2461 ggcgagaaca ggtgggtcgc ctgggatccc atgccatggg cgtcaacaaa caactgggga
     2521 ttccagcggc tgatctcgcg aatccggccg cgggtctcgg ggtgcacccc cagagcccaa
     2581 tcccggttca ggtcgaacag gtagtggttc ccgcgcccgt agggccagta tccttcgtgg
     2641 accagcgatt ggtcgtcgag gttcggcaaa gcgccgcggt gctcggcgat catcttcacg
     2701 aaccggtcgc gcccgtccgg attcatcagc ggatcgatga tgatcacgag gttggccagc
     2761 agttgcgctg tcgtcgagtc ctccgccgcg accaggtgat aagcgagcgc caacgccgcg
     2821 tccgagccct cagtctcgtc gccgtgaatc gtgtgcgcga gccaggcaac tgccggcagt
     2881 gtgtcgatca agccgcgcgc gtccgcttcg ctgaggctgc gcggatcgcc aagacgagcc
     2941 aagctcgatt gaatctggtc gagccgggca aggttggcag gggccgtgac cagcaggtaa
     3001 tggagcgggc ggttctcgtg gctgcgggcg tactcgacca ggcgtgtccg atcgggtgca
     3061 gcagcattcc aggctttgag gcagcgttcg atttcggcgg cggtgctggc gcgttccccg
     3121 gcggcaaatc cgatcaggct ctcctgggcg ggcacgtcag cccggtaggt cgcgccgggg
     3181 aaaaaggcgt tggtataagc caagcccgag actgggaccg gcgggaggcc ggccgcgggg
     3241 cgatcgtgtt ggcccaaagc ggtcgacacc aggccgacgg ccaggcaacc ggcaagacga
     3301 tgagcgtgag aaatgaaca
//
LOCUS       DAOQRO010000365         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_32876_length_3290_cov_4.794436, whole genome shotgun sequence.
ACCESSION   DAOQRO010000365 DAOQRO010000000
VERSION     DAOQRO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32876_length_3290_cov_4.794436"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKK20_11780"
     CDS             <1..1009
                     /locus_tag="PKK20_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNV00609.1"
                     /translation="ATNQGSAGLPQEAWRGLHVYLSDREDLPVMIRALEEQLAPNGLN
                     ALVVEVDFNFQYQSHPELGSKSGMTKADVRQLLAVCRRHGIELIPQFQCFGHQGSRPN
                     VFLSTYPELMAPPKPDYSDPNHYHVSWNPLDPRVNEIIFPLFDELIEAFEPKYFHVGM
                     DEVMLFPDETTPHYNGETPAEVFARAANDYHDYLVKQKGLTMLMWGDRLIDRSRIRYN
                     WMESSSNGTAPAIDMIPKDIIVCDWHYNRMPDYPSIGFFQEKGFRVWPATWKSPSAAM
                     ALLDSAKRQAQGDRMLGYMCTTWCDPGDFARAILGESENDEPSVAKRVAETFRVISEQ
                     W"
     gene            complement(1026..1433)
                     /locus_tag="PKK20_11785"
     CDS             complement(1026..1433)
                     /locus_tag="PKK20_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00610.1"
                     /translation="MIIIHGDADFVVPYYTDVLFAGRKFDVLPVPETVRFWAEINGCR
                     PEPIIEPIPNTVPGDGTTSVKETYYDDNGVPRVVHIRVRNGGHTWPGGYEPLPPFMTG
                     KQSKDFSATAMIWDFFSLHRLKTPGPAHREPRP"
     gene            complement(1459..2745)
                     /locus_tag="PKK20_11790"
     CDS             complement(1459..2745)
                     /locus_tag="PKK20_11790"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HNV00611.1"
                     /translation="MERASGVDLEAVELALRQALLREGVCVLETLLESVGVGRREQAV
                     RCRCGTPMRSVGVRTKQVQTLLGNVRFARSRYRCPECGKTRCPGDEELGIERTSRSPG
                     VQRQVARLGSKEPFREVARDLQELAGIRLSRKDAERVSERIGAEMEQWQERERMALRF
                     TEPPPPEAPKTIETLYVEFDGTGVPMVPNELAGRKGKQPDGSAKTREAKLGCVFTQTA
                     FDNEGRPIRDSGTTSFVGAIENAQTFGERIFAEAVRRGLYQAKRVVVLGDGAEWVKNQ
                     AGIHFGLAQYIIDFYHASEHVTDLCRALFDRDLKRLNRYRDRWLDALEDGAIETIVDQ
                     AREFLPKDPKSGKDARTQIGYFEKNKEYMRYARFKAEGYFIGSGVIEAGCKTVIGQRL
                     KQSGMEWTVRGANAIIALRCATLSRRAEEFWEQRAG"
     gene            complement(2754..3119)
                     /locus_tag="PKK20_11795"
     CDS             complement(2754..3119)
                     /locus_tag="PKK20_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00612.1"
                     /translation="MKRQNEILETIKGIRTMRRGTLSKQAYPDRAKRKNGKGAVGPYC
                     LWQGTVGGRRFGKRVSGKEAQRIEEGISRRHAFEALCEEYTELSCRLAELDKQGTGCE
                     QTLKKGLKSRSSKAGKSRG"
BASE COUNT          600 a         1036 c          958 g          696 t
ORIGIN      
        1 cgcgacaaac cagggctcag ctggcttgcc gcaggaagcc tggcggggac tccacgtcta
       61 tctcagcgac cgcgaggacc tgccggttat gattcgcgcg ctcgaagagc aactggcgcc
      121 gaatgggctg aacgcgctcg tggtcgaggt ggatttcaat ttccagtacc agtcccatcc
      181 cgagctggga tcgaagtcgg gcatgaccaa agcggatgtg cggcagttgc tggccgtgtg
      241 ccggcgccat ggaatcgagt tgattcctca gttccagtgt ttcgggcacc aggggagccg
      301 tccgaatgtc tttttgagca cgtacccgga attgatggcg cccccgaagc ccgattacag
      361 cgatccgaac cactaccacg tgagttggaa tccgctcgac cccagggtaa acgagatcat
      421 ttttccgctg ttcgacgaac tgatcgaggc gtttgaacca aagtattttc acgtcgggat
      481 ggatgaggtc atgctgttcc ccgacgaaac cacgccgcat tacaacggcg agacgcccgc
      541 cgaggtgttt gcccgggccg ccaatgacta ccatgattat cttgtcaaac agaagggcct
      601 caccatgctt atgtggggcg atcgcctcat cgaccgctcg cggatccggt acaactggat
      661 ggaaagcagc agcaacggca ccgcgcccgc cattgacatg attccgaagg acatcatcgt
      721 ctgcgactgg cattacaacc gcatgccgga ctacccgtca atcgggttct tccaggagaa
      781 ggggtttcgc gtatggcccg ccacctggaa gtcgccctcc gccgcaatgg cccttctcga
      841 cagcgccaaa cgccaggctc agggcgaccg catgctggga tacatgtgca ccacctggtg
      901 cgatcccggg gatttcgccc gggccatcct tggagagtcg gagaacgacg aaccgagcgt
      961 ggccaaacgc gtcgccgaga cctttcgggt gatctcggag cagtggtagg gtcccggaaa
     1021 tcacttcagg gacgcggctc gcggtgtgcc gggcccggcg tcttcagccg gtgcagcgaa
     1081 aagaagtccc agatcatcgc tgtcgcactg aaatctttac tctgtttgcc cgtcatgaag
     1141 ggcggcaggg gctcgtagcc gccgggccag gtgtggcccc cgttgcggac cctgatatga
     1201 accactctgg gcacgccgtt gtcgtcgtag taggtctctt ttacggaggt ggtcccgtcg
     1261 ccgggtacgg tgttcgggat cggttcgata atgggctcgg ggcggcaccc gttgatctcc
     1321 gcccagaacc tgacggtctc gggcaccggc agcacgtcga acttcctgcc cgcaaacagc
     1381 acgtccgtgt agtacgggac cacaaaatcg gcatcgccgt ggatgatgat caacgggcct
     1441 gcgtcacaaa cgtgggtttt agcctgcgcg ctgttcccag aactcctctg cgcggcggga
     1501 tagggtggcg cagcgcaggg cgatgatggc attggcgcct ctgacggtcc actccatacc
     1561 ggattgtttg aggcgttggc caataacggt cttgcagccg gcttcgatga cgccggagcc
     1621 gatgaagtaa ccttcggctt tgaagcgggc gtagcgcatg tactctttgt tcttctcgaa
     1681 gtagccgatt tgggtccggg catccttgcc cgattttggg tctttgggaa ggaattcgcg
     1741 ggcttggtcg acgatggttt cgatggcgcc gtcttcgagg gcatcaagcc atcggtcgcg
     1801 atagcgattg agacgcttga ggtcgcggtc gaacagggcg cggcacaggt cggtaacgtg
     1861 ctccgaggca tggtagaagt cgatgatgta ttgggcaagg ccgaagtgaa tcccggcttg
     1921 attcttcacc cattcggcac cgtcgcccag cacgaccacc cgtttggctt ggtaaaggcc
     1981 gcgacgcacg gcctcggcaa agatccgttc cccgaacgtc tgggcattct caatggcgcc
     2041 gacgaaggaa gttgtgccag agtcacggat aggccgccct tcattatcga aagcggtctg
     2101 cgtaaacaca caccccagtt tggcctcgcg ggttttggcg cttccgtcag gttgtttgcc
     2161 tttgcggccc gccagttcgt tgggcaccat cggcaccccc gtgccgtcga actccacata
     2221 cagggtctca atggtcttag gcgcttcggg cggcggcggc tcggtgaaac gtagcgccat
     2281 tcgttcgcgc tcctgccact gctccatctc ggcgccaatg cgctccgata cccgttcggc
     2341 gtctttgcgg ctcaaccgta tcccggccag ttcctgcagg tccctggcca cctcccgaaa
     2401 gggttctttg gaacccaaac gggccacttg ccgttgcacg ccgggcgaac gcgaagtccg
     2461 ttcgatcccc agttcttcgt cccccgggca gcgggttttg ccgcactcgg ggcatcggta
     2521 acgactgcgg gcaaaccgga cattgcccag caacgtctgc acctgttttg tccgaactcc
     2581 cacactgcgc atcggggtcc cgcagcggca gcgcacggct tgctcccgtc gacccacccc
     2641 aacgctttcg agtaaggtct cgagcacgca caccccttcc cgcaacagcg cttggcgcaa
     2701 agccaactcc accgcttcga gatcgactcc cgacgcccgc tccaccgctc gcactacccg
     2761 cgcgacttcc cggctttgct cgaccgcgac ttgagccctt tttttagcgt ctgttcgcaa
     2821 cccgtcccct gtttgtcaag ctcagcgaga cgacagctca actcagtgta ctcttcgcac
     2881 agggcctcga atgcgtgccg acgtgagatt ccttcctcaa tgcgctgcgc ttccttgccc
     2941 gacacgcgct tgccgaaacg cctgccgcca accgtaccct gccagaggca ataggggcct
     3001 actgcgcctt tgccgttttt gcgtttggcg cgatcgggat acgcctgctt gctcaacgta
     3061 ccgcgccgca tagtccggat acctttgatg gtttcgagta tttcgttctg ccgtttcatg
     3121 atttgctggg ccgtgataga cataacatgg cctcctttcg gtgtatatcg cattatacac
     3181 caaaggcccc caattcgcca cccctcatca aaacactcgt ttatgacgca ggccccctcc
     3241 gtcccggcgg tttgcgggaa taatggccct ttttccggca tttgctatac
//
LOCUS       DAOQRO010000366         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_32904_length_3288_cov_5.478194, whole genome shotgun sequence.
ACCESSION   DAOQRO010000366 DAOQRO010000000
VERSION     DAOQRO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32904_length_3288_cov_5.478194"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /gene="argS"
                     /locus_tag="PKK20_11800"
     CDS             complement(<1..1350)
                     /gene="argS"
                     /locus_tag="PKK20_11800"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNV00613.1"
                     /translation="MLHRFIARRLRDAIAPILPDADLSSVEVRPCRDPRHGDYQSSAV
                     IGLAKARRLNPRQLAADLVRSLDVSEWCEPVEIAGDGFLNFRIRTEALDAVLQRAVQG
                     EHLFFERASQPRTIVVDYSSPNVAKPMHVGHIRSTILGDCLVRTLRLLGHRVIADNHI
                     GDWGTQFGKLLLGWKRDLDRDALERDAVGEMERLYRAINAQCDANPDTLAAAREELVR
                     LQNGDPENLALWQDMNARSQHQFDSIYARLGIRFDTTLGESFYNPRLKAVVEELEQAG
                     VARESEGAKVVFFEDDAELQKHPAIVQKSDGAANYATTDLATLAYRIETWNPDEIVYV
                     TDARQQLHFRQIFAILRRWRPQARVKLAHVWFGSILGPDGKPFKTRSGDTVKLADLLD
                     EAEERALRLVTSKNPALPETERREIARVVGLGALKYADLLPNRQSDYVFDWDKMLALT
                     "
     gene            complement(1416..1898)
                     /locus_tag="PKK20_11805"
     CDS             complement(1416..1898)
                     /locus_tag="PKK20_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV00614.1"
                     /translation="MNLAEILQADTIVSDLAASDRWQVIDELIDCLVAAGKIKLEHRD
                     AVVAVVRKRETSMSTGIGFGIGIPHASTDLIDDVVGAFGRSRKGIQFDALDNQPVTLV
                     MLFLVPQGQFQKHLHTLADIAKLLHRKEFRQALEQAPDASTMYGIIRNNATEKPPRPV
                     "
     gene            2045..>3288
                     /locus_tag="PKK20_11810"
     CDS             2045..>3288
                     /locus_tag="PKK20_11810"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV00615.1"
                     /translation="MRSRSDSGKTAAEMTAGLRRYLYFTAACCGASILIVEILGAKML
                     APYLGTSHFVWTAQIAITMVALASGYATGGWLADRWPTLRWIYASLLMAAAWLCGAAL
                     VCEPVAFACLRLRLATGSLLAATVLFFLPLGVLAMVGPVFVRALTRSLTTVGQSMGRL
                     TSVSTIGSVAGTMLIGYVLIPFLPNSTTLCATAGVLVLLAAVYFLLWDRRHAGGVGTG
                     VAGCVLLLYLGASQRPFASVPGLTELHRRNSNFGLMQVVENRSGTRRYYLNDLLTQNG
                     YDPVRRQSASLFTHMLHGLSVAYAPHATNILCIGMGIGIVPMQLAQAGVQVEVVEINP
                     AVIPLAQQFFDFKPEAVRIHVGDGRYFLQTATNRYDVVVLDAFLGESPPSHLMTRESF
                     ESVRRCLVPNGVLRRHRAQDGD"
BASE COUNT          546 a         1013 c         1075 g          654 t
ORIGIN      
        1 cgtcagcgcg agcatcttgt cccagtcgaa gacgtaatcg ctctggcggt tgggcagaag
       61 atcggcgtac ttgagcgccc ccaagccaac cacccgggcg atctcgcgcc gttccgtctc
      121 gggaagcgcg ggattcttgg atgtgacgag ccgaagggcg cgttcctcgg cttcgtccag
      181 caaatccgcg agcttgaccg tgtcgccgga tcgggtcttg aagggcttgc catccgggcc
      241 caggatggat ccgaaccaga cgtgggccag ttttaccctc gcctgggggc gccagcgcct
      301 aagaatcgcg aaaatctggc ggaagtgaag ctgctggcgt gcgtcagtga catagacgat
      361 ctcgtcgggg ttccaggtct cgatccggta ggccaaggtg gcgaggtcgg tggtggcgta
      421 attggcggcc ccgtcgctct tctgaacgat ggcggggtgt ttttggagtt cggcatcatc
      481 ctcgaagaac accactttcg cgccttcgct ctctcgggcg actccggcct gttcgagttc
      541 ctcgacgacc gccttgagcc gcggattata gaaactctcg cccagcgtgg tgtcgaagcg
      601 gataccgagg cgggcgtaga tggaatcgaa ctggtgttgg gagcgcgcgt tcatgtcctg
      661 ccacagcgcg agattctccg gatcgccgtt ctgaagccgg acgagttcct cccgtgccgc
      721 cgcgagcgtg tccggattcg cgtcgcactg ggcgttgatg gcgcggtaga gccgctccat
      781 ctcgcccacg gcatcccgtt cgagggcgtc gcgatcgagg tcgcgcttcc agcccagaag
      841 cagtttgccg aattgcgtgc cccaatcgcc gatgtgattg tccgcgatca cgcgatgccc
      901 cagcagacgc agcgtccgga ccaggcagtc gcccagaatc gtcgatcgga tgtggccgac
      961 atgcatcggt ttggccacgt tcggcgagct gtagtcgacc acgattgtcc gcggctggga
     1021 tgcccgttcg aagaacaggt gctcgccctg aacggcacgc tggagcaccg cgtccagggc
     1081 ttccgtgcgg attcgaaaat tcaggaagcc gtcgccggca atctcgaccg gttcgcacca
     1141 ttccgacaca tccagagacc tcacgagatc ggccgccagt tggcgcgggt tgagccggcg
     1201 cgctttcgcc aatccgatga ccgccgaact ctggtaatca ccgtgtcgcg gatcgcggca
     1261 cgggcgcacc tcgacgctgc tcagatcggc atcggggagg attggcgcga tggcgtctcg
     1321 gaggcgtcgt gcgatgaaac ggtgcagcat gatggagacg ggacggggcg atgaggcaac
     1381 ctggctgtgg ggcgatgccg gctgcccggc gctgatcaaa cggggcgcgg cggcttttcg
     1441 gtggcgttgt tccggatgat tccatacatg gtggaagcgt ccggggcctg ctccaaggcc
     1501 tggcggaact cttttcgatg cagcagcttg gcgatgtcgg ccagggtatg cagatgcttc
     1561 tggaactgtc cctggggcac caggaacagc atgaccagag tcacaggctg gttgtcgagt
     1621 gcgtcaaatt gaatcccctt ccgcgagcgg ccgaaggcgc cgacgacgtc gtcgatgagg
     1681 tcggtcgagg catggggaat cccgatgccg aagccgattc cggtgctcat cgaggtctcg
     1741 cgcttgcgga cgaccgcaac gacggcatcg cggtgctcca gcttgatctt gccggccgcc
     1801 accagacaat caatgagctc gtcgatcacc tgccaacgat cgctggccgc aagatcgctg
     1861 acgatggtgt ccgcttggag gatctcagcc agattcatag acatcagatc aaatacgcat
     1921 ttcgcaccaa gcctcccggc atttcaagtg aatttaagac cgaatctgac actgacacgc
     1981 cgacacaccc gccgtccctc tgcaactgct ggacgggatc ggaggggatt ggtacaaagc
     2041 cgacgtgcgt tcgcgatccg actcaggcaa gacggcggcg gagatgaccg ccgggctgcg
     2101 ccgctatctg tatttcacgg ccgcgtgttg cggggcctcg atcctgatcg tcgaaatcct
     2161 gggcgcgaag atgctcgccc cgtatctggg cacatcccat tttgtctgga cggcccagat
     2221 cgccatcacc atggtcgcgc tggcgtcggg ctacgcgacg ggaggatggc tggcggatcg
     2281 atggccgacg ctgcggtgga tctatgcgtc gctgctcatg gctgccgcat ggctgtgcgg
     2341 cgcggctttg gtgtgcgaac cggtcgcgtt tgcctgcttg agactgcgcc ttgccacggg
     2401 ctctttgctg gccgccaccg tgcttttctt cttgccgctt ggagtcctcg cgatggtcgg
     2461 gccggtgttc gtgcgggcgc tgacgcgttc gctgacaacc gtcggccaga gcatggggcg
     2521 gctgacctcg gtcagcacga tcgggagcgt ggctgggacg atgctgatcg gctacgtcct
     2581 gatcccgttt ctgccgaact cgaccaccct ctgtgcgacg gccggcgtgc tggtcttgct
     2641 ggcggcggtc tactttctgc tttgggacag gcgtcacgcg ggcggggtgg ggaccggcgt
     2701 ggcggggtgt gttctgctcc tttacttggg ggcaagccag cggccgttcg cgtcggtgcc
     2761 cgggttgacc gagttgcacc gccgcaactc gaatttcggg ctcatgcagg tggtcgagaa
     2821 ccgatcgggc acccgccgct actatctgaa cgatctcctc acacagaacg gttacgaccc
     2881 ggtgcgccgg cagagcgcct cgctgttcac gcacatgctg cacgggttgt ccgtcgccta
     2941 tgcgccccac gccaccaaca tcctgtgcat cggcatgggc atcggaatcg tgccgatgca
     3001 attggcccaa gctggcgtcc aggtcgaggt tgtcgagatc aatccggccg tcatcccgct
     3061 cgcccagcag ttcttcgact tcaagcccga ggcggtccga atccatgtcg gcgatggccg
     3121 ctattttctc caaaccgcca caaaccgcta cgacgtcgtc gtgctcgacg cgttcctggg
     3181 ggaatcgcct ccctctcacc tcatgacgcg ggaatcgttc gaatccgttc ggcgttgtct
     3241 ggtgcccaac ggcgtcctgc ggcgccatcg agcacaagat ggggatca
//
LOCUS       DAOQRO010000367         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_32958_length_3285_cov_3.808669, whole genome shotgun sequence.
ACCESSION   DAOQRO010000367 DAOQRO010000000
VERSION     DAOQRO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32958_length_3285_cov_3.808669"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PKK20_11815"
     CDS             <1..1188
                     /locus_tag="PKK20_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV00616.1"
                     /translation="RFVDQILSEALRLQATDIHLEPYNATIKLRYRIDGVMQAVPVPS
                     ELRQVYGALVSRLKVMASLNIAEKRVPHDGRITMKTEDEEYDLRVSIVPTKHGESVCL
                     RVLGRQSLMLELKELGLETAQEALIQELTQLPQGLVLLTGPTGSGKTTTLYAALAHAN
                     DEGRKIITIEDPIEYQLEGVVQIQTREEVGLSFASGLRSVLRHDPDVVLIGEIRDSDT
                     AEIAVRAAQTGHLVFSTLHTNDSISAITRLLDMGIDPFLLGSSLVCSIAQRLAQRICP
                     HCKRPEPDLPTDLRQEMAAALGLAPESVEAFRGEGCIECQLKGHRGRVGIYEFFVMTD
                     EVVDLLGPSIKTGQLRAAARVHGWRTLREHAWSKVQAGLVSISEIQRLTRRIKPSALE
                     AAG"
     gene            complement(1185..1706)
                     /locus_tag="PKK20_11820"
     CDS             complement(1185..1706)
                     /locus_tag="PKK20_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00617.1"
                     /translation="MKLDVKDGADASAPPAASGSRPRSAASGWTPVVGLSALKAFGAV
                     FVFFLVLTVRCADTEAEAVDPTGIWKWSYAAGTGNVLEAEIRLRRVDARLVGEAVGAD
                     RKELPLEKIEFKDGRLRFEVTRLVVGKRFTTRYEGVITGDRIKGKSRAGSGASAPSRD
                     WEAVRVRAGAVSD"
     gene            complement(1715..2800)
                     /locus_tag="PKK20_11825"
     CDS             complement(1715..2800)
                     /locus_tag="PKK20_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNV00618.1"
                     /translation="MESTGNGLNRRQFGRTLALAGAGLAINAGGRAQVPARLGVKLGF
                     DNFSVRALGLKAGGLLDYAASQKLDSLYITSLDALESLEERYLREVRAKAADLGIDIL
                     LGTWSICPTSRAFNKKWGTAEEHLALGIRTAKALGSPVLRVVLGTREDRKSEGGIEAR
                     IADTVRVCQSARSQALDAGVKISVENHAGDMQAWELVTLIEAAGKDYVGATFDSGNST
                     WALEDPMASLEVLAPYIVSTHIRDSMVWEYEDGAKVQWTAIGEGCIDSPAFFAKLARL
                     CPGMPVHIEIISGFAVDFPYLKPGFWDAWPKARGADFARFLALAKRGKPLAPHRSPDN
                     TAEQEYQKSELERSLKYCKEVIGLGLR"
BASE COUNT          620 a         1195 c          882 g          588 t
ORIGIN      
        1 cgttttgtcg accagatcct ctccgaggcg ctccggctcc aggccaccga catccacctc
       61 gaaccgtaca acgccacgat caaactgcgc taccgcatcg acggcgtcat gcaggccgtc
      121 cctgtcccgt cggaactccg ccaggtctat ggcgctctgg tctcgcgtct gaaggtcatg
      181 gcgagcctca acatcgccga aaaacgcgtc ccccacgatg ggcgcatcac catgaagacc
      241 gaggacgagg agtacgatct gcgagtctcg atcgtgccaa ccaagcacgg cgaatcggtc
      301 tgcttgcgcg ttctcggacg ccagagcctc atgctcgaac tgaaggaact cggtctcgaa
      361 accgcgcagg aagcgctcat ccaggaactc acgcaattgc cccaaggcct tgtcctgctc
      421 accggcccca ccggcagcgg caagaccaca accctctacg ccgccctcgc ccatgccaac
      481 gacgaaggcc gcaagatcat cacgatcgag gaccccatcg agtatcaact cgagggcgtg
      541 gtccagatcc agacccgcga ggaagtgggc ctctcctttg ccagcggatt gcgctctgtg
      601 ctccgacacg atcctgacgt tgtgttgatt ggcgagattc gagattccga caccgctgaa
      661 atcgccgtgc gcgccgccca gaccggccac ctggtcttct ccaccctcca caccaacgac
      721 agcatcagcg ccatcacgcg tctcttggat atgggcatcg atccgttcct gctcggctcc
      781 tcgctggtct gcagcatcgc ccagcgcctg gcccaaagga tctgccccca ctgcaaacgg
      841 cctgaacccg accttccgac cgatcttcgc caggagatgg ccgcggctct cggcctggct
      901 cccgaatcgg tcgaggcctt tcgcggcgag ggatgcatcg agtgccagct caaaggccat
      961 cgcggacgcg tcggcatcta cgagttcttc gtcatgaccg acgaagtggt cgacctcctc
     1021 ggcccgagca tcaagaccgg ccagttgcgc gccgccgcgc gcgtccatgg ctggcgcacg
     1081 ttgcgcgaac acgcctggtc gaaagtccaa gccggactcg tctcaatctc cgaaatccaa
     1141 cggctcaccc gccgcatcaa accgtccgcc ctcgaagccg ccggctagtc cgacactgcc
     1201 cccgcccgca cacgaaccgc ttcccagtcg cgcgatggcg ccgatgcccc agaccccgcc
     1261 ctcgacttcc ccttgatccg gtcgcccgtg atgacgccct cgtatcgggt cgtgaacctc
     1321 ttcccaacaa ccagccgcgt gacctcgaat cgcagccgac catccttgaa ttcgattttc
     1381 tcaagaggca actcctttcg atcggccccc acggcctcac cgaccagtct ggcgtcgacc
     1441 ctgcgaagtc gaatctccgc ttccaacacg ttgcccgtcc cggccgcgta actccatttc
     1501 cagatgccgg tcgggtccac cgcctccgcc tccgtgtccg cgcaccgcac cgtcaacacg
     1561 agaaagaaaa cgaagaccgc cccaaacgcc ttcagcgcgc tcaggccaac cacgggcgtc
     1621 caaccgctcg ctgcgcttcg tggtcgtgaa cctgaagcgg cgggaggtgc ggacgcgtca
     1681 gcgccgtctt tcacatcgag tttcatggtc tgtctcaccg caagcccaat ccgatgacct
     1741 ccttgcagta cttgagactg cgttcgagct cgctcttctg atactcctgc tccgccgtgt
     1801 tgtccggaga acgatggggc gccagcggct ttccccgctt cgccaaggcc agaaaacgcg
     1861 cgaagtcggc cccccgcgcc ttgggccagg catcccagaa tccgggcttg agataaggaa
     1921 aatcaacagc gaacccggaa atgatttcga tatggaccgg catgcccggg caaagccggg
     1981 cgagcttggc gaagaacgcc ggcgaatcga tgcatccttc cccaatcgcc gtccactgca
     2041 cctttgcgcc atcttcatac tcccaaacca tcgagtcgcg gatgtgcgtg ctgacgatgt
     2101 agggcgcgag gacctcgagg ctcgccatcg gatcctcgag cgcccaggtc gagttccccg
     2161 aatcgaacgt ggcgccgaca tagtccttgc ccgcggcctc gatcagcgtg accaactccc
     2221 aagcctgcat gtcgcctgca tggttttcga ccgaaatctt gacaccggca tccagcgcct
     2281 gggaccgggc actctgacac acgcgcaccg tgtccgcgat ccgcgcctcg atgccgccct
     2341 cggacttgcg atcctcgcgc gtgcccaaca caacacgaag cacgggcgaa ccgagggcct
     2401 tggctgtccg gatgcccagg gccaaatgtt cctcagccgt cccccacttc ttgttgaacg
     2461 cgcgtgacgt agggcagatg ctccacgtgc caagcagaat gtcgatgccc aggtccgccg
     2521 ccttcgcccg cacctcccgc agatatcgct cctcgagact ctcgagcgca tccaggctcg
     2581 tgatgtagag cgagtcgagc ttctgcgacg cggcgtaatc gagcaacccg cccgccttca
     2641 gcccgagggc ccgcaccgag aaattgtcga agcccagttt cacccctagc cgggccggca
     2701 cctgggcgcg cccccccgcg ttgatcgcca gcccggcccc ggccaaagcc agggtccgac
     2761 caaactgacg ccgattcaga ccatttcctg tagactccat gagcaactcc atcaacggtc
     2821 gggcagtcgg ctcgaacccg cccgggggct acccggctgt ctcagcggtc catccgccgc
     2881 ccgcttcacc gcccttggtg gcatagatta cgccacgctg gcaagcgtga tccactctgg
     2941 gagttgagag ttgagagtcc agagttggga gttggaggtc aaacgtttct cgttcattgt
     3001 ctccgtgcgc attcaaccgt tcaatgtcat cgattcccat gaagcgctcc aggctccgag
     3061 agactccccc cgcacctgcc gtacacatcc ggcctcactc tgtaattgcg cggacgctcg
     3121 attcgctgtt tcctcgcacc caacaatgaa ccccggaagg caatcatggc tgtaaacact
     3181 ttgaatctgt cggttgccgg cccgatctcg gcggcagtgg accgcgtgcg gctcgtcctg
     3241 ttccaaccgt tcgacctcga gaagtggctg acactgggct tctgc
//
LOCUS       DAOQRO010000368         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_33180_length_3271_cov_4.143346, whole genome shotgun sequence.
ACCESSION   DAOQRO010000368 DAOQRO010000000
VERSION     DAOQRO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33180_length_3271_cov_4.143346"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKK20_11830"
     CDS             complement(<1..346)
                     /locus_tag="PKK20_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00619.1"
                     /translation="MELTRLNPCSPPTSRAVFSQYSAAHPRPGDRADRDGRAAPAEQI
                     AVALVKRRSAPGGRREERNPENLEAGAIDVFGSVETDIAKATEGIDPASQTVIRATKQ
                     RHTVFDSPEGGVG"
     gene            365..460
                     /locus_tag="PKK20_11835"
                     /pseudo
     CDS             365..460
                     /locus_tag="PKK20_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(481..1620)
                     /locus_tag="PKK20_11840"
     CDS             complement(481..1620)
                     /locus_tag="PKK20_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNV00620.1"
                     /translation="MCRNGRIASIERLAEATPTDYWIAPGLLDLQINGYAGVDFQADV
                     VSLDGLLTAARGLRRAGCHRFLATLVTDAWERMLDKLERLRALRNRSPELTEAIVGWH
                     IEGPFLSEQPGYCGAHDPGRMCDPLPERIRQLRAAAGDDLLLLTLAPERSGAIEAIAL
                     AISLGIRISLGHTDASADRISQAIEAGASGFTHLGNGCPQTLDRHNNILWRILETRSL
                     TVSMIPDGRHVSPSLFRLAHRLLPSRALVYITDAMAAAGAPPGRYALGEMQLEVGDDQ
                     VVRVPGQPYFAGSALRPVDGVWRAATMTRMPWPRAWWRFSQRPAQWLGLRSGLAVGQR
                     ADFCLLKQTGNEMPTRIRVFCGGEEAEPSSNPHSRHRARSDRPGR"
     gene            complement(1886..3091)
                     /locus_tag="PKK20_11845"
     CDS             complement(1886..3091)
                     /locus_tag="PKK20_11845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNV00621.1"
                     /translation="MTTALLRPGDADRVLAGHPWIYAGSILRLTGPAADGDVVQIKDH
                     RQRFLGIGMFNSRSKIRIRWLAPDRVAIDRRFFEQRLRNALAVRRRHMPDATSYRVVN
                     AESDFLSGLIVDKYEESLVLQISSLGMDRRKADIVAALEATLAPKTIVERSDVASRKF
                     EGLSPAQGTLFGPETTTVQARMNGLAFEVSLLEGHKTGLYLDQQINYLRVAELARGIP
                     NANVLDCFSFTGGFGLHAARAGAARVHMLDLSEEALAIARRQADANGLGACCSFEACN
                     VFDWLKTRTTTGPHEKLVPVFDVIVLDPPSFTRNRAAVPDALRGYKEIHLRALKLLKP
                     GGLLATFCCSHHVDARTFLDVLLSAAFDARKLLRRVATFSQSPDHPVLPSIPETEYLK
                     GYAIELANL"
BASE COUNT          602 a         1047 c         1027 g          595 t
ORIGIN      
        1 cgccaacgcc gccttccggg ctgtcgaaaa cggtgtgccg ctgcttcgtt gcacgaataa
       61 cggtctgact tgctggatcg atgccttcgg tcgccttcgc gatgtcggtt tcaacggacc
      121 caaagacgtc tatggccccg gcttcaaggt tttccggatt ccgctcctct ctcctgccgc
      181 ccggcgcgga ccgacgtttt accaacgcca cggcgatctg ttcggctgga gctgcgcggc
      241 catcacggtc ggcgcgatcg ccagggcgcg ggtgcgcagc agaatactgc gagaagactg
      301 ctctcgaggt cggcggactg cacggattca ggcgggtgag ttccattctt cgtggtgaac
      361 ggtcttttgc gtccagtacc atcccgaggc ctcgccaggg ccacatgact cggcgcctct
      421 tttcgacgag ttccgcgcga tgatcgaacg catcggctaa accgcacatt tgacacaccg
      481 tcagcgccca gggcggtccg atcgcgcacg atgtctgcta tgcgggttag aactgggctc
      541 ggcttcttct cccccgcaaa acacccgaat ccgggtcggc atctcgtttc cggtttgctt
      601 gagcagacag aaatccgctc gctggcccac ggcaaggccg ctccgcaaac cgagccattg
      661 cgccggcctt tgcgaaaagc gccaccacgc ccgtggccag ggcatccggg tcatggtcgc
      721 ggcgcgccac actccgtcca cggggcgcaa ggcgcttccg gcgaaatagg gttggccagg
      781 cacgcgcacc acttgatcgt ccccgacctc gagctgcatc tcgcccaacg cgtagcgtcc
      841 gggcggcgcg cccgccgcgg ccatcgcgtc ggtgatatac acgagcgcac ggctgggcag
      901 gagtcgatgc gcaagtcgaa agagcgaggg cgacacgtgc cttccatcgg gaatcatgct
      961 gaccgtcaga ctgcgggttt cgagaatccg ccagaggatg ttgttatggc ggtccaatgt
     1021 ctgcgggcat ccattcccga ggtgcgtgaa gccgctggcc ccggcctcga tcgcctgcga
     1081 gattctatca gccgacgcgt cggtgtggcc caggctgatc cggatgccca gggaaatcgc
     1141 cagggcaatg gcttcgattg ccccacttcg ttccggcgcc agagtcaaca acagcagatc
     1201 gtcgcccgcg gccgccctga gttggcgaat tcgctcgggc agtggatcgc acatgcgccc
     1261 cggatcatgg gctccgcaat acccgggttg ttcagacaaa aacggtcctt cgatgtgcca
     1321 cccgacgatg gcctccgtca attcgggaga acgattccgg agcgcccgca accgttcgag
     1381 cttgtcgagc atccgctccc aggcgtcggt cacaagcgtg gccaggaacc ggtggcaacc
     1441 cgctcgacgc aatcctctcg ccgcggtcag caagccgtcg agcgacacaa cgtcagcctg
     1501 gaaatcgacc cctgcgtagc cattgatctg taggtccagc aagcctggag caatccagta
     1561 gtcggtcggc gtggcctcag ccaagcgctc gatgctcgcg atccgtccgt tgcgacacat
     1621 cacacggatg ggacgttgcg atagataatg gcgcgcgacc acgctggaca cggtcgtttc
     1681 acgcgaggag gcgcggcctt ggcgcaccga ggcgcgaggg gtccttcgac gcttgcgttg
     1741 cggtccgcga ggcgaggcgg aggaggagga cgctgtgttc attgtcactc agatgcgggc
     1801 tggcgaggcg cggctcgttt tcgagagtcc ttgtcccctt caacccagca ctgaaacatt
     1861 acctgtctgc aaccggtttc atcggtcaaa gattcgccaa ctcgatcgcg taccccttaa
     1921 ggtactcagt ttccgggatc gagggaagga ctgggtgatc gggcgactgg gagaatgtgg
     1981 cgactcgacg caggagtttg cgggcatcga aggcggcgga gagcagcacg tccaagaagg
     2041 tgcgggcatc cacgtgatgc gagcagcaga aagtggcgag gagccctccc ggtttgagca
     2101 gcttgagggc gcgcagatgg atttctttgt acccgcgcag ggcatcgggc accgccgcgc
     2161 gattgcgcgt gaacgacggc ggatcgagca cgatcacatc gaacacggga accagtttct
     2221 cgtggggccc ggtggtcgtg cgcgtcttga gccagtcgaa cacgttgcac gcctcgaacg
     2281 aacagcacgc tcccaggccg ttggcatcgg cctgtcgccg cgcgatggcc agagcttcct
     2341 cgctcagatc gagcatgtgc actcgcgccg ctcccgcccg cgccgcgtgc agcccaaacc
     2401 cgccggtgaa ggagaaacaa tcgaggacat tcgcgttggg tatgccccgc gccaattcgg
     2461 ccacgcgcag atagttgatc tgttggtcga ggtaaaggcc tgtcttgtgg ccttcgagca
     2521 ggctgacttc gaatgccagg ccgttcatcc tggcctgaac cgtcgtcgtc tccggcccaa
     2581 agagcgtccc ctgcgccggc gaaagaccct cgaacttgcg cgaggcgaca tcgctccgct
     2641 cgacgattgt tttgggcgcc agcgtcgcct cgagcgcagc cacgatgtcc gccttgcgac
     2701 ggtccatgcc cagcgacgaa atctgcagca ccagcgactc ctcgtacttg tcgacgatca
     2761 gcccgctcag gaaatcgctc tcggcgttga cgacgcgata ggacgtcgcg tccggcatgt
     2821 ggcggcgtcg cacggccagg gcgttgcgaa ggcgttgctc gaaaaaccgc cggtcgatcg
     2881 caacccggtc gggcgccagc catcggatac ggattttcga tctcgagttg aacatcccaa
     2941 tgccgagaaa ccgttgccga tggtccttga tttgcaccac gtctccgtcg gctgccggtc
     3001 cggtcaggcg caggatcgag ccggcataaa tccatggatg tcccgccagc acgcggtccg
     3061 cgtcgcccgg acgcaagagc gccgttgtca tggattgcat ggttgcgtgc ataagtcggt
     3121 tcaagggcgg caggcccggc ccgccgcaaa gaacacacat caagacacac cgccgccctc
     3181 caaggaagct caaaccggag agccaaccca gaccttcacg gtcttttcat ttggagcggt
     3241 gcttgacgag tatctggaaa gggatttggg g
//
LOCUS       DAOQRO010000369         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_33625_length_3243_cov_4.073714, whole genome shotgun sequence.
ACCESSION   DAOQRO010000369 DAOQRO010000000
VERSION     DAOQRO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33625_length_3243_cov_4.073714"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKK20_11850"
     CDS             complement(<1..556)
                     /locus_tag="PKK20_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNV00622.1"
                     /translation="MKSRPRAVDRILDALRKHRTFCIVGHVRPDGDCVGCQLALAMAL
                     QNQGKEVTVWNEDRLPDKLAFLDPDKLFQLPRPGESFDCVVAVDAANYERLGRVGDCV
                     RSRACLINIDHHTSNTRFGDINWVDARAPSTGELLFRLLGCASWPVTPRIADCLFTAV
                     STDTGSFQYPSTRPATFTVAGALVK"
     gene            complement(635..1006)
                     /gene="rbfA"
                     /locus_tag="PKK20_11855"
     CDS             complement(635..1006)
                     /gene="rbfA"
                     /locus_tag="PKK20_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNV00623.1"
                     /translation="MQSRRHERVRELIKRELSSILLREFPVSEAGLLTVNEVALAGDL
                     QSAVVYVGVVGSPEQRRQGLAALNQQRARIQSMVASAVVLKYMPQLRFVADEAIARGN
                     RVLEIIEEIERAAPPKSAPAN"
     gene            complement(1071..>3243)
                     /gene="infB"
                     /locus_tag="PKK20_11860"
     CDS             complement(1071..>3243)
                     /gene="infB"
                     /locus_tag="PKK20_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNV00624.1"
                     /translation="QRPGRVDLAVEIPRPDAEKAPEGSTPAVEALPPSPPPPPPGPKL
                     GDKIGFIQLPNRPRFADRSGGKGTQRSTATRPDAARSRDPRGGQPAAPRPSGAPGQRA
                     GTPAAPPAREPGRWATLPPLPVATGPEISLKPPIIVRDLAEKLGKKPFQLIADLMELG
                     VFATVNQAIESEHAQRVCAKYNFRFRVEKRDKGVSRIHRPLPKKEPEDTEDRLEDLKP
                     RPPVVTIMGHVDHGKTSLLDVIRRSNVVAGEAGEITQHIGAYSISVPHPERPKELCPI
                     TFLDTPGHAAFSAMRARGAHVTDIVVLVVAANDGVMPQTLEALSHAQAAKVAIVVAVN
                     KCDHPNAEPMRVRQQLQERGLTPDDWGGDTIFVDVSAITKQGIDKLLGSILLQAELLE
                     LKANPSRPAKGNVVESGLEPGGPTATLLVRKGTLQVGQLLLCGQYYGKVRALIDEGGK
                     RLKAAEPSRAVKMLGLNGVPEAGAEFVVVESEKVARALCEERAAAHRDQEVEHRPKVT
                     LETIFQTMEEEQAKILRLIVKADTQGSIEAIVDSLRQIDSDKVSLDVIYSGVGAVTKE
                     DVLLASASKGIILGFHTRIDGSVADLAKREGVQIKLYAIIYELIDQVKEAMVGLLDPL
                     QREVVFGVAEVRQVFTLSKGGHVAGCVVLSGRLVKGKARVMRRRNLIHEGFIQTLRRY
                     QDDANEVRAGMECGIRFDGFDGFLEGDTIECYTIEVVRQQL"
BASE COUNT          518 a         1003 c         1102 g          620 t
ORIGIN      
        1 gtttgaccag tgcgccggcg acggtgaaag tggccggccg cgtcgacggg tactgaaatg
       61 agcccgtgtc ggtcgagacc gcggtgaaaa ggcaatcggc gatgcggggc gtgacgggcc
      121 aggaggcgca gccgaggagg cggaagagga gctcgcctgt cgagggcgcg cgggcgtcca
      181 cccagttgat gtctccgaaa cgcgtgttgc tggtgtgatg gtcgatgttg atcaggcagg
      241 cccggcttcg gacgcaatcg cccacccggc ccaggcgttc gtagttggct gcatcgacgg
      301 cgacgacgca atcgaacgac tcgcccgggc gcggcagttg aaagagcttg tcgggatcga
      361 ggaaagccag cttgtcgggc aacctgtcct cgttccatac cgtcacctcc ttgccctggt
      421 tttgcagggc catggcaagc gccaactgac acccgacgca atccccatcc ggacgcacat
      481 gtccgacgat gcagaaggtc cggtgttttc ggagggcatc gagaatgcgg tcgaccgcgc
      541 ggggtctgga cttcatggcg cgaaacggat ggccgacaga ggcatcggcg gcgggcgatt
      601 cgaggggaat gcggcggagc gcatcgcctt cacctcagtt ggccggggcc gatttcggcg
      661 gtgcggcgcg ctcgatttcc tcgatgattt cgagcacgcg gttgccgcgg gcgatggcct
      721 catcggcgac gaatctgagt tggggcatgt atttgagcac cacggcgctg gccaccatgc
      781 tttggattcg cgcgcgttgc tgattcaggg ccgcgagacc ttgcctgcgc tgctcgggcg
      841 agccgacgac gccgacgtag acgacggcgg actggaggtc gccggcgagg gccacctcat
      901 taacggtgag cagaccggcc tcggagaccg gaaactcgcg cagcaggatg ctgctgagct
      961 cccttttgat cagttcgcga acgcgttcgt gtcgtcggct ttgcatgatc gaaatcgggc
     1021 ggcgaatcgg gcggcgggca tccgggagcc gaggcgcccg ggagcgcccc tcagagttgc
     1081 tgtcgaacga cctcgatcgt gtagcattcg atcgtgtcgc cctccagaaa accgtcgaag
     1141 ccgtcgaacc ggatgccgca ttccatgccg gctcggactt cattggcgtc gtcctggtat
     1201 cttcgaagcg tctggatgaa gccttcgtgg atgagattgc gtcggcgcat gacgcgggcc
     1261 ttgcctttga ccaggcgtcc gctcaggacg acgcacccgg cgacgtggcc gcctttggac
     1321 aaggtgaaca cctgccgcac ttcggccacg ccgaacacca cctctctttg cagcggatcg
     1381 agcagtccca ccatggcctc tttcacctgg tcgatcagct cgtaaatgat ggcgtagagc
     1441 ttgatctgca cgccttcgcg cttggccaga tcggccaccg atccgtcgat gcgtgtgtgg
     1501 aatccgagga tgatgccctt ggatgcggag gccaggagga catcctcctt ggtgacggcc
     1561 cccacgccgc tgtagatcac atcgagcgag accttgtcgc tgtcgatttg ccgcagcgaa
     1621 tcgacaatgg cctcgatcga tccctgcgtg tccgctttga cgatcaatcg gaggatcttg
     1681 gcttgctcct cttccatcgt ctggaagatg gtttcgagcg tgactttcgg acgatgctcg
     1741 acctcctgat cccgatgcgc ggcggcccgc tcttcgcaaa gggcgcgcgc gaccttctcg
     1801 ctttcgacca cgacaaactc ggcgccggcc tcgggcactc cgttgagtcc gagcatcttc
     1861 acggcccgcg atggttcggc ggccttcaac cgcttgccgc cctcgtcgat cagcgcgcgc
     1921 accttgccgt aatactggcc gcagagcaga agctgcccaa cctgcagcgt tcccttccga
     1981 accagcaggg tcgccgtggg accgccgggt tcgagtcccg actcgacgac attgcctttt
     2041 gcggggcgac tgggattggc tttgagttcg agcaattcgg cttggagcag gatgctgccg
     2101 agcagtttgt cgatgccttg cttggtaatg gccgagacgt cgacgaagat ggtgtcgcct
     2161 ccccagtcgt cgggcgtgag gccccgttcc tgcaactgct ggcgaacccg catcggctca
     2221 gcgttcggat gatcgcattt gttcaccgcc acgacgattg ccaccttggc cgcctgggcg
     2281 tggctgaggg cttcgagcgt ctgcggcatg accccgtcgt tggccgccac cacaaggacg
     2341 acaatgtctg tgacgtgagc gccccgggca cgcatcgcgc tgaaggccgc gtggcccggt
     2401 gtatcgagga acgtgatcgg gcagagctct ttcggccgct cgggatgggg tacgctgatg
     2461 ctgtaggcgc cgatgtgctg cgtgatctct ccggcctcgc ccgccacgac gttcgatcga
     2521 cggatgacgt ccaggaggct ggtcttgccg tggtcgacat gccccatgat ggtgaccacg
     2581 ggagggcgcg gcttgagatc ctcgagccga tcttccgtgt cctccggttc cttcttgggg
     2641 agcggccgat gaatcctgct gacgcctttg tcgcgcttct cgacccggaa acggaaattg
     2701 tactttgcgc agacccgctg cgcatgctcg gattcgatag cctggttgac cgtcgcgaag
     2761 acgcccagtt ccattaaatc ggcaatcaac tggaacggtt tcttgcccag tttctcggcc
     2821 aaatcgcgga cgatgatggg cggcttgagc gagatttccg gccccgtcgc aaccgggagg
     2881 ggcggcaggg tcgcccaccg gcccggctca cgggctggag gcgcggcggg tgttccggca
     2941 cgttgcccgg gcgcgccgga gggtcggggc gccgccggct gcccgccgcg gggatcgcgg
     3001 gatcgagccg cgtcgggtcg ggtggctgtc gagcgttggg tgcccttgcc gcccgatcga
     3061 tcagcgaagc gaggccggtt ggggagttga atgaacccga tcttgtcgcc cagtttcggc
     3121 ccgggagggg gagggggagg actgggcggg agggcttcga cagcgggcgt ggaaccctcc
     3181 ggggccttct cggcgtccgg acgtggaatc tccaccgcca gatccactcg gcccggccgc
     3241 tgc
//
LOCUS       DAOQRO010000370         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_33748_length_3235_cov_4.573270, whole genome shotgun sequence.
ACCESSION   DAOQRO010000370 DAOQRO010000000
VERSION     DAOQRO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33748_length_3235_cov_4.573270"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1452)
                     /locus_tag="PKK20_11865"
     CDS             complement(79..1452)
                     /locus_tag="PKK20_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV00625.1"
                     /translation="MPVKPETNQTARHRILIVDDDPGQRSLLESLLRGQGFDTVVVAS
                     GEQALDALRTQLVSMMISDVRMPGLSGLETLRLARQAHPRLPVLLVTAYADIRDAVGA
                     MRDGALNYLTKPIDFDELLASVRQAIGLTQAPPMRFGERHQLPAHVVACSPLMQAVFR
                     DASLIAPSDSRILITGESGVGKEVLAEVIHAWSARANGPLVRVNCAALPENLLESELF
                     GHEKGAFTGAHAQRIGRFELAGKGTIFLDEIAEMSPQLQAKLLRVTQDGCFQRVGSNA
                     DRVTQARLLAATNRDLEDEVRQGRFREDLFYRLNVVELHVPPLRERIEDILPLAHAFL
                     ADYTGGRTRLSNPAVECMKRYVWPGNVRELCNAMERAALLCGGELILLDHLPARVRAT
                     TASAPTAPPTEGQQLEEVERQTILEALRKHGHNRTETAKALGISRRALIYKLQRFREL
                     GFDVDAP"
     gene            complement(1517..3214)
                     /locus_tag="PKK20_11870"
     CDS             complement(1517..3214)
                     /locus_tag="PKK20_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV00626.1"
                     /translation="MNRRSFLRRSAAALPLFTWAPLLADEDRQGGRERAADLVIVGGG
                     LGGCAAALAACGAGLRVILTEPTDWIGGQLTSQAVPPDEHPWIESFGCTRAYRQFREA
                     VRAYYRNHYPLTPEARANPRLNPGNGWVSRLCHEPRVALAVLEQMLAPHVSGGRLRLL
                     LEHEPVSALMGADRVRAVTLRNRRTDGRVTLTAPYFVDATELGDLLPMTGTEHVLGAE
                     GRADTGEPHAAAVADPRNQQAITSCFAVDYLPEEDHTIERPSEYAFWRDYVPDLKPGW
                     TGRLLSWDSTHPHTLKPRSLMFDPTMKAAGVNLWSYRRIADAANFVPGTFSSGISLVN
                     WPQNDYWVAPLIENTPDEIARNVQRAKELSLSLLYWMQTEAPRPDGGVGWKGLRLRHD
                     IVGTTDGLAKHPYIREARRIRAEFTVAEQHIGTDARMQATGKSRADVTAEPFADSVGV
                     GAYRIDLHPSSSGENYLDLSSLPFQIPLGALLPQRVENLLPAAKNIGTTHITNGAYRL
                     HPVEWNIGEAVGYLVAYCLRSKEPPRQVRRDAKRLAEYQQSLRNAGCELEWPRVRPL"
BASE COUNT          546 a         1022 c         1099 g          568 t
ORIGIN      
        1 gacgcaccga cgcaccgacg caccgacgca ccgacgcacc gacgcaccga cgcactgtcc
       61 cctccatctc cgaccgagtc acggtgcatc gacgtcgaac cccagttcgc ggaaccgttg
      121 gagtttgtag atgagggcgc gtcggctaat gccgagggct ttggccgttt cggtgcggtt
      181 atgaccgtgt ttgcgcaggg cttcgaggat tgtctgacgt tcgacctctt cgagttgctg
      241 cccttcggtc ggcggcgcag tgggggcgga cgccgtggtc gcccggacgc gagcggggag
      301 atggtcgagg aggatcaact cgccgccgca gagcagcgcg gcccgttcca tggcattgca
      361 cagctcgcgc acgttgcccg gccagacata acgtttcatg cactcgacgg ccggattcga
      421 caggcgcgtg cgcccgccgg tgtagtcggc caggaaggca tgggcaaggg gcaggatgtc
      481 ttcgatccgt tcgcggaggg ggggcacgtg aagttcgacc acgttcagcc ggtagaacag
      541 gtcctcgcgg aaccgccctt gcctgacctc gtcttcgagg tcgcggtttg tggcggccag
      601 caggcgggcc tgggtgaccc gatccgcatt cgatccgacg cgctggaagc acccgtcctg
      661 cgtgacacgg aggagcttgg cttgcagttg cggcgacatc tcggcgattt cgtccaggaa
      721 gatcgtgccc ttgccggcca actcgaatcg tccgatccgt tgggcatggg cgccggtaaa
      781 ggcccctttc tcatggccga acaactcgct ttcgagcagg ttctcgggca gggcggcgca
      841 gttcacgcgc accaacggtc cattcgctcg cgcgctccag gcgtgaataa cctccgccag
      901 cacctccttg cccacgccgc tctccccggt gatcagaatg cggctgtccg acggcgcgat
      961 gagcgaggca tcccgaaaga ccgcctgcat caatggactg caggccacca catgagccgg
     1021 caactgatgg cgctctccga agcgcatcgg cggcgcctgc gtgaggccga tcgcctggcg
     1081 cacggacgcc aacaactcgt cgaaatcgat cggtttggtg aggtagttca gtgctccgtc
     1141 gcgcatggcg cccacggcat cgcggatgtc ggcgtaagcg gtcaccaaca aaacaggcag
     1201 ccgcgggtgt gcctgacggg ccaggcgcaa cgtctcgagc ccgctcaacc cgggcatccg
     1261 cacatccgag atcatcatgc tgacgagctg agtccgcaag gcgtcgaggg cttgttcgcc
     1321 cgatgccacc acgacggtgt caaagccctg gccgcgaagc agcgattcga ggaggcttct
     1381 ctggcccgga tcatcgtcga cgatcaagat gcgatgccgc gccgtctgat ttgtctctgg
     1441 cttgacaggc atgatgcgag attgcgcgcg ggcagtgtag tgcgcccgtc gaacgctgca
     1501 accacgttcg cccgatttac agcggcctga ccctgggcca ttcgagttcg cagccggcgt
     1561 tacgcaggct ctgctggtac tcggcgaggc gcttggcatc gcggcgcacc tggcgcggcg
     1621 gttccttgct gcgcaggcaa taggccacaa ggtatcccac cgcctcgccg atgttccatt
     1681 cgaccgggtg caagcggtag gccccattgg tgatgtgcgt cgtcccgatg ttcttggcgg
     1741 cgggaagcag gttctcgacg cgttgtggca gcagcgcacc gagcgggatc tggaacggaa
     1801 gcgaactgag atcgagatag ttctcccccg aagaactggg gtgaagatcg attcggtagg
     1861 cgccgacgcc gacgctgtcc gcaaagggtt ccgcggtcac gtccgcgcgg gatttgcccg
     1921 tggcctgcat ccgcgcgtcg gtgccaatgt gttgttcggc gacggtgaat tccgctcgga
     1981 tgcgccgggc ctccctgata taggggtgct tggcgaggcc gtccgttgtg ccgacgatgt
     2041 cgtgtcgcaa gcgcagccct ttccagccga ctccgccatc gggacgcggc gcctcggttt
     2101 gcatccagta gagcagggag aggctgagtt ccttggcccg ttgcacgttc cgggcgatct
     2161 cgtccggtgt gttttcgatc aaaggcgcaa cccagtagtc gttttgcggc cagttcacca
     2221 aggaaatccc gctcgagaag gtcccaggaa cgaaattggc cgcatcggcg atccggcggt
     2281 aagaccacag attgacgccc gcggccttca tggtggggtc gaacatcaaa ctgcgcggtt
     2341 tgagggtgtg aggatgtgtc gagtcccaac tcaacaggcg ccctgtccag cccggtttca
     2401 aatcgggcac gtaatcccgc cagaaggcat attcggatgg ccgctcgatg gtgtggtcct
     2461 cttcagggag ataatcgacg gcgaagcagg aggtgatggc ctgttggttc ctggggtcgg
     2521 cgacggcggc ggcgtgcggt tctccagtgt cggcgcgtcc ctcggcgccc aggacatgct
     2581 cggtccccgt catcggaagc aggtcgccca actcggtggc atcgacaaag tacggggctg
     2641 tcagtgtgac gcggccgtcc gtgcgccggt tgcggagcgt caccgcccgg acacggtcgg
     2701 cgcccatcag agccgagacc ggctcgtgct cgaggagaag gcgcagtcgc cctccgctga
     2761 catgcggggc cagcatctgt tcgagcacgg ccaaggcgac gcgcggctcg tgacacaggc
     2821 gcgacaccca gccgttgcct ggattgagcc gcgggttggc ccgcgcttcc ggcgtcagcg
     2881 gatagtggtt gcgatagtaa gcgcggacgg cctcgcggaa ttgccgataa gcgcgggtgc
     2941 atccgaagct ctcgatccaa ggatgctcgt ccggcggcac ggcttgcgat gtgagctggc
     3001 cgccaatcca gtcggtcggc tcggtgagaa tgacgcggag tccggcgccg caggccgcaa
     3061 gcgcggcggc gcacccgcca agcccgccgc cgacaatgac gaggtccgcg gcccgctcgc
     3121 ggccgccctg gcggtcctca tccgccagaa gcggggccca ggtgaacagg ggcagtgcgg
     3181 cggcgctgcg ccgaaggaaa gaacgtcggt tcataccgct gagcgtagcg gtcaa
//
LOCUS       DAOQRO010000371         3232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_33786_length_3232_cov_4.601511, whole genome shotgun sequence.
ACCESSION   DAOQRO010000371 DAOQRO010000000
VERSION     DAOQRO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33786_length_3232_cov_4.601511"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            527..751
                     /locus_tag="PKK20_11875"
     CDS             527..751
                     /locus_tag="PKK20_11875"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNV00627.1"
                     /translation="MGPTLSVLIGGALGAALGYFGQCNSGTCPLTATWWRGGLFGGGM
                     GLLLFLSGENRHPRSGLIHDPPGLMKRSSL"
     gene            complement(994..1128)
                     /locus_tag="PKK20_11880"
     CDS             complement(994..1128)
                     /locus_tag="PKK20_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00628.1"
                     /translation="MSPTLVDAPRISNITADSEGAIRLLVEGVYGRAIAVEVSEDLAN
                     "
     gene            complement(1125..1490)
                     /locus_tag="PKK20_11885"
     CDS             complement(1125..1490)
                     /locus_tag="PKK20_11885"
                     /inference="COORDINATES: protein motif:HMM:NF037830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00629.1"
                     /translation="MKFLLDAHISPSVAAAMAQLGSRAVVLPIQNWHEGMYVAQHGES
                     DLPWLRMAGREGWIIVHDDRNTLLRDYALLAREGCEMPGFAVVGHAHRADVGWIARRL
                     VELERRCAKEKPGNVQVFL"
     gene            complement(1474..1923)
                     /locus_tag="PKK20_11890"
     CDS             complement(1474..1923)
                     /locus_tag="PKK20_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00630.1"
                     /translation="MKTVRAPRPTGRRPVRFNLPDDYDVRLASHEAAAGRPRNELARE
                     WAQERLDLLDFPCLEFRSNSAGRFAVVRGTRLAVWHLAALARTLGWGDDLAQHLNILP
                     EAVASAEGYYQRHRAEVEALIAANEAMGFDEVKHLLCQVRRLSEIPA"
     gene            2218..2355
                     /locus_tag="PKK20_11895"
     CDS             2218..2355
                     /locus_tag="PKK20_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00631.1"
                     /translation="MNHRAAERAEGGLFSAKPKRPILDVQQDGVIDHALFLDTTPNLE
                     A"
     gene            2365..2604
                     /locus_tag="PKK20_11900"
     CDS             2365..2604
                     /locus_tag="PKK20_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00632.1"
                     /translation="MVIGSHSVTLGLDVEMRNLTSINGDYFDTSTFAIINTGDSTLIN
                     PGRVLVQGIGRQPRFGAIDTFANQGTLEARDGPPC"
     gene            2667..2795
                     /locus_tag="PKK20_11905"
     CDS             2667..2795
                     /locus_tag="PKK20_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00633.1"
                     /translation="MTNAPVLVGDMYQLPIDFSAPHQFYRIGPPLGVLGLPAVKTR"
     gene            2871..>3232
                     /locus_tag="PKK20_11910"
     CDS             2871..>3232
                     /locus_tag="PKK20_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNV00634.1"
                     /translation="MNRILFKEQLAAEVFRFQIEAPLIAAGRKPGQFVILQQDTEFGE
                     RIPLTIADADPAKGTISVIFQRVGDGTQRLAELEAGDCITNLVGPLGRPTHIDKFGLV
                     VCVGGGIGVAPLYPIAQA"
BASE COUNT          606 a         1038 c          986 g          602 t
ORIGIN      
        1 gtcagatgca aggctcgagg gagggagtgt atcccctgag atactcgacc gacctcggtt
       61 ggcctttcgc tgttttggtt ggtgcctcag cggatcatcc ggcttcgaag gggcctgatc
      121 cgatcgatga cagcgcgcat cggcgggcgt cgagcgttcc tgcgagcggg gcctcttgac
      181 gcatcggaat aacggcctgc cagcgtcctc ctcgtctgag gaaagccccg gtggaacggg
      241 agatctgttc gcattcacgg agcctgaggt tgcagatgca gcggctttcg agcaagcacg
      301 gcgtgcggcc gcgcatctgg ttgttgtcgg tgttctgtcg cccggtgagc gcgccggacc
      361 tacaacggat cgagattgcc ggaggggcgg cgaggagatt cccgctccaa cgccttcgtg
      421 tgcgcgaacg gagcacacca ggccgcgtct tcccacgcgg cgcatactcg attcccgcag
      481 ggttgagacg taacctttgg ccgtcccgag gctcaatcac ggcatgatgg ggccaactct
      541 gagcgtgttg atcggtggcg cactcggagc tgcgctcggc tacttcggcc agtgcaactc
      601 ggggacctgt cccttgacgg ccacgtggtg gaggggaggc ctcttcgggg gaggtatggg
      661 actgctgctt tttctttcag gcgagaatcg acaccctcga tccgggctaa ttcatgatcc
      721 gcccggcttg atgaagcgct cttccctctg agtgctctgc gcctctgtgg tgaatcccat
      781 ccgatgactg gcaccattcc gaattgaacc acagagacac agagagcgca gggggcgggc
      841 caatcaatgc caagcggaag cgcccgatcg tggttgccca cctctgccag gtcaccgcaa
      901 cactgcgcgg tagaaaccgt acgcaacacc cgcggacgtc gggaactcga cctcgaccga
      961 ggattccacc cacaccgggt cggcgcacac acttcagttg gccaagtcct ccgagacttc
     1021 aaccgcgatc gcccggccat aaaccccttc gacgagaagg cgaatcgcac cctcgctgtc
     1081 ggcggtgatg ttcgagatgc gcggcgcgtc caccagtgtg ggactcacaa gaacacctgc
     1141 acattgcccg gcttctcctt tgcgcagcgg cgctcgagct ccaccagtcg gcgtgcgatc
     1201 cagccgacat cagcgcggtg tgcgtggccg accacagcga atcccggcat ttcgcagcct
     1261 tcgcgggcca gcaaagcgta atcccggaga agcgtgtttc tgtcgtcgtg gacgatgatc
     1321 cagccctcac ggccagccat tcgcagccag gggaggtcac tctcgccgtg ttgggcgacg
     1381 tacatcccct cgtgccagtt ttgaatcggc agaaccaccg cacggctccc caactgagcc
     1441 atcgcggcgg cgacggacgg gctgatatgg gcatcaagca ggaatttcac tcagccggcg
     1501 cacttggcac agcaggtgct tgacctcgtc aaagcccatt gcctcgttgg cggcgatcag
     1561 ggcttcgacc tctgcccggt ggcgctggta gtatccctcg gcggatgcca ccgcctcagg
     1621 tagaatattc aggtgttggg cgaggtcatc gccccatccc agagtgcgcg ccagcgccgc
     1681 caggtgccaa accgccagac gcgttccgcg gacgacagca aaccgccccg cggagttgga
     1741 gcgaaactcc agacacggaa aatccagcag gtccaaccgc tcctgcgccc actcgcgcgc
     1801 caactcgttg cgcgggcgcc cggcggcagc ctcatgactg gctaggcgca catcgtaatc
     1861 gtcgggcagg ttgaaccgga ccggtcgccg accagttgga cgaggcgctc gaactgtctt
     1921 cacagggggc cagtctaaca cgctcgggca cacacgcaag gacccgtgct ggaccgaccg
     1981 gacgcaagtc ctccatgcca gcgcctcagg acgtgatgag atcatccctc cgcccgcgcc
     2041 tggaagcctg gaaacgacgc gcgggaatag acgacaccgt tgaaagcctg aacagttcct
     2101 cctcgacttg gctctgcgct ggtttcgctt ccgcccccga attcataagg cgctcttccc
     2161 tctgtgtgct ctgcgcctct gtggtgaatg ccattcgatg actgtcaccg ttccggattg
     2221 aaccacagag ccgcagagcg cgcagaggga gggctattca gtgccaagcc caagcgcccg
     2281 atcctcgatg tccagcagga cggtgtgatc gaccacgcac tgttcctgga cacgacgccg
     2341 aacctcgaag cgtaagtcgc gtttgtggtc atcggtagcc actcggtcac actggggctc
     2401 gatgtcgaaa tgcggaatct cacctcgatc aacggcgact attttgacac cagcacattt
     2461 gcgatcatca acaccggcga ctcgacactc atcaacccgg gccgggtgct ggttcagggg
     2521 attgggcggc agccgcgttt tggagccatc gacaccttcg ccaaccaggg gactctcgag
     2581 gcgcgagatg ggccaccctg ctgatcgatc gagagttcac tcagagcgcc gggcggattc
     2641 tcctggtcaa cggagccagg ttcacgatga ccaacgcccc cgtcctcgtc ggggacatgt
     2701 accagttgcc gatcgacttc tccgcgccgc accagttcta tcggatcggg ccgcctcttg
     2761 gcgtcttggg tctgccagcg gtcaagaccc gttgaaaccc caaaaagaaa gttgtcgcct
     2821 gtggggccac aagaaactaa aaggaccgcg tcgtatctgg tctccgtgcc atgaacagga
     2881 ttctcttcaa agagcagctt gcggcggagg tcttccgctt ccagatcgag gcccccctga
     2941 tcgccgccgg ccgcaagccg ggccagttcg tgatcttgca gcaggacacc gagtttggcg
     3001 aacgcattcc tctgacgatt gcggatgccg atccggcgaa gggaaccatc agtgtgatct
     3061 tccagcgtgt gggtgacggc acccaacgcc tggcagaact cgaggcaggg gattgcatca
     3121 cgaatctggt cggtcccctg gggcgtccca cgcacattga caagttcggc ttggttgtgt
     3181 gcgttggggg cgggatcggc gtcgctcccc tctatcccat tgcccaggcg at
//
LOCUS       DAOQRO010000372         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_33901_length_3226_cov_4.846421, whole genome shotgun sequence.
ACCESSION   DAOQRO010000372 DAOQRO010000000
VERSION     DAOQRO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33901_length_3226_cov_4.846421"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKK20_11915"
     CDS             complement(<1..642)
                     /locus_tag="PKK20_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNV00635.1"
                     /translation="MRSRIPFTPVVYEHAARFVGRTPWEVSRDAGLLFEAHRAAYLEY
                     RQQVIAVGIDIYNLEAEAYGARVEVVEDDAIPAIRQPLLGCPEDGHDLPPFDPACAGR
                     IPMVLAVGQRLRREFPEADVRIPVAGPFSIAFNLRGITRLCEDAILRPEATARLLLRL
                     AENQAGFCRAIAEAGLDVAFFESAAAPPLLSPRQFHQIELPALRRIVNLAAECL"
     gene            complement(642..1382)
                     /locus_tag="PKK20_11920"
     CDS             complement(642..1382)
                     /locus_tag="PKK20_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00636.1"
                     /translation="MKPVKGAFTLIELLVVIAIIAILAGLLLPALSRAKENGKRAKCL
                     NNLRQIGIGMTVYAGDNNDLVIEARNGEVQICLNPPERGAAATIGLTITTNSTVSQIW
                     TCPNRPTFPTYEAEYPQWVIGYQYFGGIPKWINPAGTFNSRSPIRASISQPTWALAAD
                     AVMKIDGKWGGGRDSAFKDMPPHRGRGGIPDGGNVLTMDGSARWIRFDKMLFIHSWSP
                     GGDRDAYFYQEDLGPDIEKNIARLRPRP"
     gene            complement(1577..>3226)
                     /locus_tag="PKK20_11925"
     CDS             complement(1577..>3226)
                     /locus_tag="PKK20_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNV00637.1"
                     /translation="HPDFSVSLWAKGDYSIQTDRRVFAEGSTKNANPLFNIGTHNGGT
                     DGSVDIYIRTDTGGTVGDHHHSAGIAFDGDWHHILYTQRDLGGGVTTAHLYIDGVLDT
                     MKPDPVRPLTLDTTTIGGILRNTSSAWFTGQIDDVIVWNRGLTAAEAQLLATAPMPDP
                     PPNFQPLVISAFTSSMPEVVEGDSVRLRWDVRRTAEQILIDNGVGDVTSMSVSGAGSV
                     SVTPTATTTYTLTIKRGEEQLSARTTVTVLKGVEANWAALDNFDEHSPGPISRTAWWS
                     DTRGEYAQVENLNGNHMLSVRTTDSAVVLPIGSLKITEGQERTLFFRILVREPLPTAA
                     LRHVIGLTDKNIRSQGDTTSNIGPVLHANFDTAAPPWFLGVINYVGGAIEYATDPLET
                     GALYSVWIDIKNEPMNDPVSPYDIFSVYIKKDGAEERTELWKDYLSDRDALTPDPVLG
                     PMMPDLDKLFVTGNNTAASLWFDDFFISKSGYNATEPVPFNQGGTPEVSIAIQGANVV
                     LTFTGILTAADQLTGPWTDVAETSPLTISASGAQKFYRARR"
BASE COUNT          577 a          985 c         1037 g          627 t
ORIGIN      
        1 cagacactcg gcggccagat tgacgatgcg tcgcaacgcc ggcaactcga tctggtgaaa
       61 ctgcctcggc gaaagaagcg gcggcgcggc ggccgattcg aaaaacgcaa cgtcgaggcc
      121 ggcctccgcg atcgcgcgac agaacccggc ctggttctcg gccaaccgaa gcagcaggcg
      181 cgcagtggcc tcgggccgca ggatcgcatc ctcacaaagt cgggtgatgc cgcgcaggtt
      241 aaaggcgatg ctgaaaggcc cggcaaccgg aatgcgaaca tcggcctctg ggaattcccg
      301 tctcagacgc tgcccgacag cgaggaccat ggggatgcgt cccgcgcagg cagggtcaaa
      361 aggcggcagg tcatgaccat cctcaggaca gccgagcaat ggctggcgga ttgcggggat
      421 ggcatcgtcc tcgacaacct cgacgcgcgc gccgtatgcc tcggcttcga ggttgtagat
      481 gtcgatgccg accgcgatca cctgctgccg atactcgaga taagctgcgc gatgggcctc
      541 gaagagcagt cccgcatccc tcgaaacctc ccacggcgtt cgtcccacga agcgagcggc
      601 gtgctcgtag acgaccggag taaacggaat gcgcgaccgc atcacggtct aggtcggagc
      661 cgggcaatgt tcttctcgat gtcgggtccc aggtcttcct ggtagaagta cgcatcccga
      721 tccccgccgg ggctccagct atggatgaaa agcatcttgt cgaatcgaat ccaccgcgcc
      781 gagccgtcca tggtcagcac gttgccgcca tccgggattc cgccccgccc gcgatgcggc
      841 ggcatgtcct tgaatgcgct gtcgcggcct ccgccccact tgccgtcgat cttcatcacg
      901 gcatccgccg ccagcgccca ggtcggctgc gagatcgagg ctctgatcgg gctgcgggag
      961 ttgaacgtgc ccgcggggtt gatccatttc ggaatgccgc cgaagtactg gtaaccgatg
     1021 acccactgcg ggtattcggc ttcgtaggtt gggaatgtgg gacgattggg gcaggtccag
     1081 atctggctga cggtgctgtt cgtcgtgatg gtcaagccga tcgttgccgc cgcgccccgt
     1141 tcgggcggat tgaggcagat ttgcacctcg ccattccggg cctcgatcac cagatcgttg
     1201 ttgtccccgg cgtaaactgt catcccaatc ccaatctggc gcagattgtt gaggcatttg
     1261 gcacgcttgc cgttctcctt cgcccgagac agggctggca acagaagacc ggcaagaatc
     1321 gcgataatcg caatcaccac cagcaactcg atcagagtga aggctccctt aactggtttc
     1381 atagcaccaa tatgtttctc cgctgcgtgg gagagagagc attgacttca acgacttaat
     1441 gcataccggg accatgaacc aaaacggaga acggacaacg gacaacgtcc aactctcaac
     1501 tctcaactct caactcgtct tgattctccg ccggcgcctg gggttcgtca ccccgggcgc
     1561 cgagcatcct cggtctctac cttctggctc gataaaactt ctgagcgcca ctcgccgaaa
     1621 tggtcagcgg gctggtctcg gccacatccg tccacggccc cgtcaactgg tcagccgccg
     1681 tcaggatgcc tgtgaaggtg agaaccacgt tggcgccttg gatggcaatg ctgacttcgg
     1741 gcgtgccacc ttggttgaag gggacgggtt cggtggcgtt gtagcccgac ttgctgatga
     1801 agaagtcgtc gaaccagagg ctggccgccg tgttgttgcc cgtgacaaac agcttgtcga
     1861 ggtccggcat catcgggccg agcaccgggt ccggcgtcaa ggcgtcgcgg tcgctcaggt
     1921 agtccttcca cagctcggtc cgttcctctg cgccatcctt cttgatgtag accgagaaga
     1981 tgtcgtaggg cgaaacagga tcgttcatcg gctcgttctt gatgtcgatc cagaccgagt
     2041 agagcgcgcc cgtctcgagc gggtcggtgg cgtattcgat agccccgccc acgtaattga
     2101 tgacgcccag gaaccaggga ggcgcggccg tgtcgaagtt ggcgtggagc accgggccaa
     2161 tgttgctggt ggtgtcgccc tggctgcgga tgttcttgtc cgtcaggccg atcacatggc
     2221 gaagggccgc cgtgggcagc ggttcccgga ccaggatgcg gaagaagaga gtgcgctcct
     2281 gaccttcggt gatcttgagc gagccgatgg ggagcacgac cgcgctatcg gtggtccgga
     2341 cgctgagcat gtggttgccg ttcaggttct cgacttgggc gtactcgccg cgggtgtcgc
     2401 tccaccaggc ggtgcgcgag atggggcccg ggctgtgctc gtcgaagttg tccagcgccg
     2461 cccagttggc ctcgactcct ttgaggaccg tcaccgtcgt ccgggcgctc agttgctcct
     2521 caccccgttt gatggtcaat gtgtaggtgg tcgtggctgt cggcgtcacg ctgacgctcc
     2581 ccgccccgga gacgctcatc gaggtgacat cgccgacgcc gttgtcgatg agtatctgct
     2641 cggccgttct ccggacatcc cagcgcaacc tgacggaatc gccttcgacc acttccggca
     2701 tgctcgaggt gaacgcgctg atcacgaggg gttggaagtt gggaggcggg tcgggcatgg
     2761 gggccgtcgc caggagttgc gcctcggccg ccgtcagccc gcggttccac acgatcacat
     2821 cgtcgatctg tcccgtgaac caggccgagc tggtgttgcg caggatgccg ccaatggtcg
     2881 tcgtgtcgag ggtgagaggt cgcacgggat ccggcttcat cgtgtccagc acaccgtcga
     2941 tgtaaaggtg cgcggtcgtc acgcctccgc ccaaatcgcg ctgcgtgtag aggatgtgat
     3001 gccagtcgcc gtcaaaggcg ataccggccg agtgatgatg atcgcctacc gtgccaccgg
     3061 tatcggtccg aatgtagatg tccacactgc cgtcggtgcc gccattatgc gtgccgatgt
     3121 tgaagagggg gttggcgttc ttggtggaac cttccgcgaa aacgcgtcgg tcggtctgga
     3181 tgctgtagtc gcccttggcc cacagcgaca ccgagaagtc ggggtg
//
LOCUS       DAOQRO010000373         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_34190_length_3207_cov_4.475254, whole genome shotgun sequence.
ACCESSION   DAOQRO010000373 DAOQRO010000000
VERSION     DAOQRO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34190_length_3207_cov_4.475254"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..616)
                     /locus_tag="PKK20_11930"
     CDS             complement(164..616)
                     /locus_tag="PKK20_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00638.1"
                     /translation="MQPSRILPDLQASLLCEDVRQETSGNFIIIGVIGVIRVPQVPIT
                     AFKLCIFNRWTAGIGVFTQNVRIIAPDGITVLRQNPMRFGLQDASHHAVNVTVFTQIE
                     FPNAGVYHIEILVDDVMKLRYPIPLVLQPPAKGAPAADTPPPAPTETQ"
     gene            complement(653..1567)
                     /locus_tag="PKK20_11935"
     CDS             complement(653..1567)
                     /locus_tag="PKK20_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNV00639.1"
                     /translation="MTASDNLIDGRSIAEQIHRETAERIGSLKARGLAPGLTFVRVGE
                     DPASRVYVGMKERMSQRLGIRSTTEVLPEGATEGVLLELIGRLNADSGVHGILVQAPL
                     PRHIDAARVYKAVSPDKDVDGFHPVNVGKLLLGDTTGFVPCTPAGVHELLIRSGVRIE
                     GADVVVLGRGTIVGKPMAALLVQKARHANATVTVCHSLTRDIAVHCRRADILIAALGV
                     ARFVKADMIKAGAVVIDVGVNREPDSSVKTGYRLVGDVDFDSVRQVAGRLTPNPGGVG
                     PMTIAMLMQNTVRAAENSIRAPTLDSRT"
     gene            complement(1564..2679)
                     /locus_tag="PKK20_11940"
     CDS             complement(1564..2679)
                     /locus_tag="PKK20_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV00640.1"
                     /translation="MRVGPGVALALGMHGDTVAIVTGDRVNLRGRPTLSSEVVAQVLE
                     GETVVVLDSLELRPSRRGEVVQWHKIRLPEDTPVWVHAGFIDRTTKTVTASRLNVRAG
                     PGDRYSILGHLAKDESVREIRIVDDWMEIAPPSGTYAYMAASYAALQPAAVSATGPHT
                     AKTAPAATPPSVAKSVAASPHRARETPAASMIQPTPQAPPSTPAPDVQVSSMTAAVPV
                     APQSPAREPELVERPPAAVALDTVPRPEGRSGPMPGKPMPASRAALPAAPSSARRTDP
                     LWASRPASAVPAWGSHQIIQREGILLDAMHIQAPAKHKLRSLETGRDLNFVRPASVEV
                     QLTLYRGQRVIITGEERSAPGWPNLPILEARTIQLAP"
     gene            complement(2742..>3207)
                     /locus_tag="PKK20_11945"
     CDS             complement(2742..>3207)
                     /locus_tag="PKK20_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV00641.1"
                     /translation="LLTNDGDFCLRLTHPRYGVRKKYLATVEGRVTEDVLRRLEAGVM
                     ESGELLRAQRARLRSANATRSMVELELVEGKHREVRRLFESQGFPVLSLTRTQIGPIK
                     LGELPLGKYRVLAASEVRALTMAANATPERARADADAVVNAMRTGSDEGGRR"
BASE COUNT          571 a         1043 c         1034 g          559 t
ORIGIN      
        1 tgaccagcaa ggcaaccagc gccgccaccg ccagcagacg cccgccaggc gcggcagcaa
       61 acgcggcgac cccgccgaac gtcaacggtt gccacgcttc gagaggcgct tcgagaggag
      121 cggagcgggt ggaatccacg aaaggccgag gccgtctgcg ggcctactgc gtctcggtgg
      181 gcgctggggg cggggtgtcc gccgccggcg ctcccttggc gggcggttgg aggaccagag
      241 ggatcgggta gcgcagcttc atgacatcgt cgaccaggat ctcgatgtga tagacgccgg
      301 cgttggggaa ctcgatctgc gtgaagaccg tgacattgac tgcgtggtgc gaggcatcct
      361 ggaggccaaa gcgcatggga ttctggcgca gcaccgtgat gccgtcgggg gcgatgatcc
      421 ggacgttctg agtgaagacg ccaatgccgg cagtccatcg gttgaagatg cacagtttga
      481 aggcggtaat tggcacctgg gggacgcgga tcacgccgat gacgccgatg atgatgaagt
      541 tgcccgaggt ctcctggcgg acgtcctcac acaggagtga ggcttggaga tcgggaagaa
      601 tacgagaggg ttgcatagcg attcgatttc gagactgttg gattctgtgt gtctaagttc
      661 gggaatcgag ggtgggcgct cgaatcgagt tctcggcggc ccgcaccgtg ttctgcatca
      721 gcatcgcgat cgtcattgga cccactccgc ccggattggg cgtaagacgc cccgccacct
      781 gccggaccga gtcgaaatcg acgtcgccga ccaggcgata gccggtcttg acggacgaat
      841 ccggctctcg gttcacacct acgtcgataa ccacggctcc cgctttgatc atgtcggctt
      901 tgacgaatcg cgccacgccc agcgctgcaa tcagaatgtc ggcgcggcgg caatgcaccg
      961 cgatgtcccg cgtcaaggaa tggcaaaccg tgacggttgc gttggcgtgc cgggccttct
     1021 gcaccaacag cgccgccatc ggcttgccga caatcgtccc ccgcccgaga accaccacgt
     1081 ccgccccctc gatccgcacg ccagatcgaa tcagcagttc gtgcaccccg gccggtgtgc
     1141 aggggacaaa gccggtcgtg tctcccagca acaacttgcc cacattgacc ggatgaaagc
     1201 cgtcaacatc cttgtcgggt gaaaccgctt tgtacacccg cgcggcatcg atgtgccgtg
     1261 gcaatggcgc ctgaaccagg atgccgtgaa ccccggaatc cgcgttgagc cggccgatca
     1321 actcgagcaa gacgccctca gtcgcgccct cgggcaggac ttcggtcgtc gaacggatgc
     1381 ccaaccgctg actcatccgt tccttcatgc ccacatacac tcgggaagcg ggatcctcgc
     1441 cgactcgcac aaaggtcaac cccggcgcca gcccgcgcgc cttcaaggag ccaatgcgct
     1501 ccgccgtctc gcggtgaatc tgttccgcaa tcgaccgccc atcgatgaga ttgtcgctcg
     1561 ctgtcatgga gccaactgaa tcgtccgcgc ctctaagatc ggcaggttgg gccaaccggg
     1621 cgcactccgt tcctctccag taatgatcac ccgctgcccg cggtaaagcg tcaattgaac
     1681 ctcgacggac gccggcctga cgaagttgag gtctcggcct gtttccaaag acctcagttt
     1741 gtgcttggcc ggggcttgaa tgtgcatggc atcgagcaga ataccttcgc gctgaatgat
     1801 ttgatgcgat ccccacgccg ggacggcaga ggccgggcgc gaagcccaga gcgggtcagt
     1861 gcgacgcgcg gacgaaggcg ctgcgggcag ggcggctcgg ctggccggca tgggcttgcc
     1921 aggcatcgga ccactccggc cctcgggcct cggcacggta tccagtgcaa cggcggccgg
     1981 cggtcgctcg accagttccg gctcccgtgc cggcgattgg ggagcgaccg ggaccgcagc
     2041 cgtcatggat gagacctgga catcaggcgc aggggtcgac ggaggcgctt gcggcgtcgg
     2101 ctgaatcatc gatgcggccg gagtctctcg agccctgtgc gggctcgcgg cgacggactt
     2161 tgcgacggac ggcggtgtcg ccgctggagc tgtctttgcg gtgtggggtc cggttgcgga
     2221 cactgctgcg ggttggagcg ctgcatagga agccgccatg taggcatacg tgcccgacgg
     2281 cggtgcgatc tccatccagt cgtccacgat gcgaatctca cgaacgctct cgtctttggc
     2341 gagatggccc aagatgctgt atcggtctcc cggcccggcc cgcacgttga ggcggctggc
     2401 ggtgaccgtt ttggtcgttc gatcgatgaa acccgcatgc acccacacag gggtgtcttc
     2461 gggaaggcga atcttgtgcc actggacgac ctcgccccgg cgagacgggc gcagctcgag
     2521 cgaatcgagc acgaccactg tttctccctc gaggacttgg gccactacct cgctggacag
     2581 ggttggacga ccccgcaggt tgacgcggtc tcccgtgacg atggccaccg tgtcgccgtg
     2641 catgccgagg gcaagagcaa cccctggccc gacccgcacg accagacccc agactcgccg
     2701 gcagatcgac ctcgaaaacg gatgctgaat ccgggccgat ctcaacgccg tcctccttcg
     2761 tccgagcccg tccgcatcgc gttcacaaca gcgtcggcat cggcccgcgc tcgttcgggc
     2821 gtcgcattcg ccgccattgt cagggcgcga acctcgctgg ccgccagaac gcggtacttg
     2881 ccaagcggca attcccccag cttgattggg ccgatttgcg ttcgcgtcag cgagagcacc
     2941 ggaaagccct gcgactcgaa caggcgccgc acctcgcgat gcttgccttc gaccagttcg
     3001 agttcgacca tgctgcgggt ggcgttggct gaacgaagcc gcgcccgctg ggcgcgcagc
     3061 agttctccgc tctccatgac gcccgcttcg agccgcctca agacatcttc ggtcacccgc
     3121 ccctcgacgg tcgccaggta cttcttgcgc accccgtatc gcggatgtgt gagccggagg
     3181 cagaaatccc catcgtttgt cagcaga
//
LOCUS       DAOQRO010000374         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_34528_length_3184_cov_5.204538, whole genome shotgun sequence.
ACCESSION   DAOQRO010000374 DAOQRO010000000
VERSION     DAOQRO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34528_length_3184_cov_5.204538"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKK20_11950"
     CDS             <1..266
                     /locus_tag="PKK20_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00642.1"
                     /translation="MEEGRRQEGGGEEWAAIRRGWFFGANELKRQLLAQASERVGAQH
                     YGAERQESGQAKAERLVQEELASLGRQESDLAARRKGDPGKPR"
     gene            complement(263..>3184)
                     /locus_tag="PKK20_11955"
     CDS             complement(263..>3184)
                     /locus_tag="PKK20_11955"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNV00643.1"
                     /translation="LMPFQFTHPWWLLLLPAVSGWVLWLASRSYVQTGPWRRRVALAL
                     RLIVAWALVLGLAGMQWKRPLEGLNVYFLLDRSDSIPSTQQEAAREYVNRAGAAKKTV
                     DKGGIVVFGTGASIETAPNSKIDVPKIHAVVGTERTDIAGAIRLGTAAFPETGQKRLV
                     LLSDGNENLGEAVGAALAARALGVSIDVVPMGISRGGDVSVQKVTVPPLLKKGQTFEV
                     KIFAQTDTNREATLRLFRNDQFLGEQKIELTAGKNLFAFPQTLTEPGFYNYDIQLEAA
                     GDRVPQNNRAGTFTSVRGDPRLLVVSADPDQDAPLAGALRASNLEVELTDLGGFPDTL
                     AELQGFDAIFISNLAAGDLGTTTMRLIESAVRDFGVGLVCVGGDQTYAAGGYRDTPLE
                     AALPVEMELSSKKVLPSGAVILIMHGMEFNNGNQIARECAQGVLDALGPQDELGVVLW
                     DGTDRFLFPLSKVGDKREKGRMIAGMNQGDLPSFQHVMEMGYKGDANSPGLKQSTANL
                     KHMIVFSDGDPGPPSDALMQAIVNDRITVSTVLIAGHAGPETMIRIADLGRGRFYDVQ
                     DPNQLPQIFLKEAMVILKSAIFEEPFQPQMVAGSELLRGIGANELPTLRGYVCTTPKG
                     RAEVPLVSDKGDPVLAHWQYGLGRAVAFTSDAKAKWAVDWMAWPKYRQFWSQVAQWAL
                     RRLDLADFNAEISVDQGEGVLSVEALDAQGNYRNFLDLQAVVVTPKGEAQTIRLEQAG
                     PGRYETRFPTREVGTYLLNLMEMQNGQVRASQALGANVSYSPEFNATEPHLNLLRRIA
                     ESGNGRLLPPLMETDANAVNPFRHDRIRTYQPRDLWEGLLKLGLLLFLADVAVRRIYL
                     DRAEWLKATENLRRILFFWRRPARPVEADESLAALLARRGEVRAKHTALDADSRPDLF
                     APSHPTSTVTLPTSGPEKAPAAAPPPAPQPSEPAPPADRAATTSRLLEAKRRAAKRMD
                     R"
BASE COUNT          514 a          948 c         1153 g          569 t
ORIGIN      
        1 agatggagga aggtcggcgg caggaaggcg gcggggagga atgggcggcg atccgacggg
       61 gatggttttt cggggcgaac gagttgaagc ggcaattact ggcgcaggca tcggagcgag
      121 tgggcgcgca gcattacggg gctgagcgcc aggagagcgg gcaagccaag gcggagcggt
      181 tggtgcagga ggagttggcg agcctgggcc ggcaggaatc ggacctggca gcgcggcgca
      241 agggcgatcc cggcaagcct cgctaacgat ccatgcgttt ggcggcccgg cgtttggctt
      301 cgagcagccg gcttgtggtg gcggcgcggt cggcgggcgg cgcgggctca gagggttgag
      361 gcgccggcgg aggcgcggcc gcgggggctt tctccggacc ggatgttggg agcgtcacag
      421 tcgaggttgg atgtgagggg gcaaagaggt ccggacgact gtcggcgtcg agggcggtgt
      481 gtttggcgcg gacttcgccg cgtcgggcga gcaaagcggc gagggattca tccgcctcga
      541 cgggccgcgc tgggcggcgc cagaagaaga ggatgcgccg gaggttttcg gtggccttga
      601 gccactcggc gcgatccagg tagatgcgtc gaacggcgac atcggcgagg aagagcaaca
      661 ggcccagctt gaggagccct tcccagagat cgcggggctg gtaggtccgg attcggtcgt
      721 gccgaaacgg gttcacggcg ttggcgtccg tctccatcaa tggcggaagc agccggccgt
      781 ttccggattc ggcgatgcgc cggaggaggt tgaggtgcgg ctcggtggca ttgaattcgg
      841 gcgagtagct gacgttggcg cccagcgcct gggaggcgcg cacctgtccg ttctgcattt
      901 ccatgaggtt gagcaggtag gtgcccacct cgcgggttgg gaatcgcgtt tcgtatcggc
      961 ccgggccggc ctgttcgagt cggatggtct gcgcttcgcc tttgggtgtg acgacgaccg
     1021 cctggaggtc gaggaaattg cggtaattgc cctgggcgtc gagcgcctcg acactgagga
     1081 cgccttcgcc ctgatcgacc gagatttcgg cattgaaatc ggccaagtcg agtcgccgca
     1141 gcgcccattg ggcgacctgg gaccaaaact gccggtactt gggccatgcc atccagtcca
     1201 ccgcccattt cgccttggcg tcggaggtga acgccacggc gcggcccaac ccgtactgcc
     1261 agtgggccag gacgggatcg cctttatcgg acacgagcgg cacctcggcc cggcctttgg
     1321 gcgttgtgca gacgtagccg cgcagtgtgg gcagttcatt ggccccgatg ccgcggagca
     1381 actcgctgcc ggcgaccatc tggggctgga aaggctcctc gaagatggcg gacttgagaa
     1441 tgaccatcgc ttccttgaga aaaatctggg gcagttggtt cgggtcctgg acgtcgtaga
     1501 accggccccg tcccaggtcg gcgatgcgga tcatggtttc agggccggca tggccggcga
     1561 tgagcaccgt gctcaccgtg atgcggtcgt tgacaatcgc ctgcatgagg gcgtccgacg
     1621 gcgggcctgg gtcgccgtcg ctgaacacga tcatatgctt gaggttggcg gtggactgct
     1681 tgaggcccgg gctgttggcg tcgcccttgt aacccatctc catgacgtgc tggaagctcg
     1741 gcaggtcgcc ctggttcatg ccggcgatca tgcgcccttt ctcccgtttg tccccgacct
     1801 tcgagagcgg aaagaggaag cggtcggtgc cgtcccagag gacgacgccc aactcgtcct
     1861 gcggacccaa tgcatcgaga actccctggg cgcattcgcg ggcgatctgg ttgccgttgt
     1921 tgaactccat gccgtgcatg atgagaatca cggcgccgct gggcaggacc ttcttgctgc
     1981 tcagttccat ctcgaccggc aacgcggctt cgagcggcgt gtcgcggtag ccgcccgccg
     2041 cgtatgtctg gtcgccaccg acgcagacca ggcccacgcc gaaatcgcgc accgcgctct
     2101 cgatcagcct cattgtcgtc gtccccagat cgcccgctgc gagattgctg atgaagatgg
     2161 cgtcgaatcc ctgcaactcg gccagagtgt ccgggaagcc gccgaggtcg gtcagctcga
     2221 cttcgaggtt cgaggcgcgc agggcgccgg ccaacggggc gtcctggtcg gggtcggccg
     2281 acaccaccag gagccgcgga tcgccccgca cgctggtgaa cgtgccggcc cggttgttct
     2341 gcggcacccg gtcgccggcc gcttcgagct ggatgtcgta gttgtagaag ccgggttcgg
     2401 tcagcgtctg gggaaaggcg aagaggttct tgcccgccgt gagttcgatt ttctgctcgc
     2461 ccaggaactg atcgttgcgg aacagacgca gcgtcgcctc gcggttggtg tccgtctgcg
     2521 cgaaaatctt cacttcgaac gtctgcccct tcttgagaag cggcggcacg gtgaccttct
     2581 gtacgctcac gtcgccgccc cgggaaatac ccatcgggac gacatcgatg ctcacgccca
     2641 gagcgcgggc ggcgagggcg gctccgaccg cttcgccgag gttctcgttg ccgtccgaga
     2701 gcagcacgag ccgtttctgg ccggtctcgg gaaaagcggc cgtgccgagc cggatggcgc
     2761 cggcgatgtc ggtgcgttcg gttcccacca cggcgtggat ttttgggacg tcgatcttcg
     2821 agttgggagc cgtctcgatg ctggcgccgg ttccgaacac gacgatgcct cccttgtcga
     2881 ctgttttctt ggccgcgccg gcccgattca cgtattcccg cgcggcttcc tgttgagttg
     2941 aagggatgct gtcggagcga tcgaggagga agtagacgtt gagtccttcg agcggacgct
     3001 tccactgcat gcctgccaaa ccgagcacaa gggcccaggc gacgatcagc cgcagcgcga
     3061 gcgcgacgcg ccgccgccag ggaccggtct ggacgtagct gcgcgaggcg agccagagca
     3121 cccaccccga gaccgcgggg agcagcagca accaccaagg atgggtgaat tgaaagggca
     3181 tcag
//
LOCUS       DAOQRO010000375         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_34573_length_3182_cov_4.780940, whole genome shotgun sequence.
ACCESSION   DAOQRO010000375 DAOQRO010000000
VERSION     DAOQRO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34573_length_3182_cov_4.780940"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1109)
                     /locus_tag="PKK20_11960"
     CDS             complement(132..1109)
                     /locus_tag="PKK20_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00644.1"
                     /translation="MKLLRLVSLSALGLLGIATAADLRIGLIGLDTSHVIAFTSLIND
                     TTHASHVPGGKVVAAFKGGSPDIESSWSRVEGYTKQLEEKYGVKIVDSIEALCAEVDV
                     VMLESVDGRPHLEQVKPVLRARKPVFIDKPVAGSLRDAIEIYRLARQHQVPCFSSSSL
                     RYYPGLVELKKTDVGVVKGAVSTGPCHLEPHHPDLFWYGIHPVEALYAVLGVGCETVV
                     RTATADTDVVTGVWKDGKVGTLIGIREAAAPYRVTLFGTKAVVDQKPGGDYAPLVREV
                     MKFFQTGVAPVPAEETIELFAFMEAADESKRQGGRPVRVADVLRQHGGQ"
     gene            complement(1260..2741)
                     /locus_tag="PKK20_11965"
     CDS             complement(1260..2741)
                     /locus_tag="PKK20_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNV00645.1"
                     /translation="MKHRHLVLWATAIALLLSSTGCISLNGADATGTPIPFGPVPTER
                     QLRWHAMEFYGFLHFTVNTFTDKEWGYGDESPRVFNPTAFDADQIARTAREAGMTGLI
                     LTAKHHDGFCLWPSRFTEHSVKNSPWKEGRGDVVREISEACRRQNLRFGVYLSPWDRN
                     HADYARPEYIRYYRNQLRELLTQYGELFTVWFDGANGGDGYYGGARERRQIDNRTYYD
                     WPNTWAIVRELQPFACMFSDAGPDFRWIGNERGIAGDPCWATLDMTKPNRHPGGSSEG
                     LNSGERPGTAWLPGECDVSIRPGWFYHPAEDSKVKTPAELLDIYYKSVGRGACLNLNL
                     PPDRRGRIHENDVASLREFRRILDAAFATDLARGAKLTASATRAGNRSFAVENVSDGN
                     RGTYWTTDDAVTTPDLVLDLGRPARLNVVSLREFLPLGQRVEAFALDAWRDGAWHEFA
                     QGTSIGNRGLIRTAPLTTDKVRLRITQAPVCPAIAEVGLFLEP"
     gene            2842..>3182
                     /locus_tag="PKK20_11970"
     CDS             2842..>3182
                     /locus_tag="PKK20_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00646.1"
                     /translation="MNRVDFMLNLAGLLLWLEWRAVRLGAPLAAAGSISAVLKHAKPR
                     AARRWLHLAGLVALLGIRCVFYWRVGSKLGWVPSLDLGTITLPFNSVSLGRMALYSVL
                     SFGVVLAVCHLG"
BASE COUNT          532 a         1011 c         1039 g          600 t
ORIGIN      
        1 aacgagaagt gtgcgtttag aagcacagcc ggagctcggg cgcaggcgcc cgcaaccgct
       61 ctcatcagcg cctgtgcatg gagggcgacc agggccgccg cgcatcggct caccgctccg
      121 gtgagggcgg ttcactggcc accgtgttgg cgcagcacat ccgccacgcg aaccggtctt
      181 cccccctgcc gcttgctctc gtcggcagct tccatgaacg caaacagttc gatggtctcc
      241 tcggcgggaa cgggagccac accggtctga aagaacttca tcacctcgcg caccagcggc
      301 gcatagtccc cgccgggttt ctgatccacg acagctttgg tgccgaacag ggtgacgcga
      361 taaggggcgg cggcttcgcg gattccgatg agggttccga ccttgccgtc cttccagacg
      421 cctgtgacga catcggtgtc cgcagttgcc gtccgcacaa ccgtttcaca gccgacgccc
      481 aggaccgcat agagcgcctc gaccgggtgg attccatacc agaacagatc gggatgatgc
      541 ggttcgagat ggcagggacc ggtcgagacg gcgcccttga ccacgccgac atcggtcttc
      601 ttcagctcca ccaggcccgg atagtagcga agggacgagg agctgaagca tgggacctgg
      661 tgttgcctgg cgaggcgata gatttcgatg gcatcgcgca gcgagcccgc aacgggcttg
      721 tcgatgaaca ccggtttcct ggcgcgcaag accggcttga cctgttcgag gtgcggacgc
      781 ccatccacgc tctcgagcat gacgacatcc acctccgcgc agagggcctc gatcgagtcg
      841 actatcttga ccccgtactt ctcctcgagt tgcttggtgt agccctcgac acgcgaccag
      901 ctcgattcaa tatcggggct cccgcccttg aacgccgcca ccaccttgcc gcccggcacg
      961 tggctcgcat gcgtcgtgtc gttgatcagg ctggtgaacg cgatcacatg cgatgtgtcg
     1021 agcccgatca gcccgatgcg cagatcggca gccgttgcga ttccaagcag gcccagggcg
     1081 gacaaagaga cgaggcggag caatttcatg ccacggagcc tagcaggccg gggatgggcc
     1141 aggcaatctc tcgatgaatc gcattcgagt cgatctgagg cgcgcctttc cagcccgcgg
     1201 cggtccgcat ctcggagtcc tcggcttcgc cgcgatccca agtgcgtgaa acatgcgggc
     1261 tagggttcta ggaacaagcc gacctcggcg atcgccggac agacgggcgc ctgggtgatg
     1321 cgcagcctga ccttgtcggt cgtgagcggc gcggtccgga tgaggcctcg attcccgatg
     1381 ctggttccct gggcgaactc gtgccatgct ccgtcgcgcc acgcgtcgag cgcgaaggcc
     1441 tcgaccctct ggcccagggg caggaactcg cgcaggctca ccacgttcag ccgggctggc
     1501 cgccccagat cgaggaccag atcgggcgtt gtcaccgcat cgtcggtcgt ccaatacgtg
     1561 ccccggttgc catcggagac gttctcgacg gcaaacgacc gattcccggc gcgcgtggcg
     1621 ctggctgtga gcttcgcccc ccgtgccaga tccgtcgcaa acgcggcgtc gagaattcgg
     1681 cggaattcgc gcagagacgc aacatcgttc tcgtgaatgc gtccgcgcct gtccggcggc
     1741 aggttcaggt tcaagcaagc gccgcgtccg acagatttgt aatagatgtc gagaagttca
     1801 gcaggggttt tgaccttcga atcttcggcg ggatgataga accagccggg gcgtatcgag
     1861 acgtcgcatt cgcccggaag ccatgccgtc ccggggcgtt cgccgctgtt gagcccttcg
     1921 ctggagccgc cggggtggcg gttgggcttg gtcatatcga gcgttgccca gcacggatcg
     1981 cccgcgatgc cccgttcgtt gccgatccag cggaagtcgg gtccggcatc gctgaacatg
     2041 caggcgaagg gctgcagttc gcgcacgatc gcccaggtgt tcggccagtc gtagtaggtg
     2101 cgattgtcga tctgacgcct ctctctcgcg cccccgtagt agccgtcgcc cccgttggcg
     2161 ccatcgaacc agacggtgaa cagctcgccg tattgggtga gcagctcgcg cagttggttg
     2221 cgatagtagc ggatgtactc gggacgggca taatcggcgt ggttgcggtc ccaaggcgag
     2281 agatagaccc caaatctcag gttctggcgg cggcaggcct cgctgatctc gcgcaccacg
     2341 tccccgcgtc cctccttcca cggtgagttc ttcaccgaat gctcggtaaa ccggctcggc
     2401 cacaggcaga atccatcgtg gtgcttcgcc gtcaggatca gccccgtcat ccccgcttct
     2461 ctggccgtcc tcgcaatctg gtccgcgtca aaagccgtcg gattgaaaac ccgcgggctt
     2521 tcgtcgccgt acccccattc cttgtcggtg aacgtgttca ccgtgaagtg aagaaacccg
     2581 taaaactcca tcgcgtgcca acgcagttga cgctccgtcg ggacgggtcc gaacgggatg
     2641 ggggtgccgg ttgcatcggc gccgttgagg gagatgcagc cggtcgaaga gaggagcaga
     2701 gcgatcgcag tggcccagag aacgaggtgc cggtgtttca tgggcgcgca gtctagcggc
     2761 ggcgcatcca ggagtccaga caggccggcg aattgatgat tgaaccctgg gttgggtctg
     2821 gacacactcg atccgctgcc gatgaatcgc gttgatttta tgctgaatct ggcggggctg
     2881 cttctgtggc tcgagtggcg ggcggtgcgg ttgggcgcgc cgctggcggc ggcgggctcg
     2941 atttccgcgg tcctgaagca cgccaagccg cgcgcggcgc ggcggtggct ccacctggcg
     3001 ggcctggtgg cgctgctggg gattcggtgt gtgttctact ggcgcgttgg ctccaaactc
     3061 ggttgggtgc cgtccctcga cctcggaacc atcaccctcc cgttcaacag cgtttccctc
     3121 gggcgcatgg cgttgtactc cgtgttgagc ttcggcgtcg ttctggccgt gtgccacctt
     3181 gg
//
LOCUS       DAOQRO010000376         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_34786_length_3169_cov_5.763327, whole genome shotgun sequence.
ACCESSION   DAOQRO010000376 DAOQRO010000000
VERSION     DAOQRO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34786_length_3169_cov_5.763327"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKK20_11975"
     CDS             complement(<1..943)
                     /locus_tag="PKK20_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HNV00647.1"
                     /translation="MSSPFLHLWAQTLRLRRTTTCAIGLALVLAAAGKAANAPAIPLP
                     EHPRPDFERAEWLNLNGSWQFRFDKDNAGLAGGWAASQEAFPLKITVPFPWGSALSGV
                     GNEADIGWYSRQIRVPAEWRGKRVFLVVGASDWLTRGWLDGQSIGEHRGGYTPFEWDL
                     TPVVKWGEDQRLVLRVDDTPHAFKLEGKQGYGPAKGIWQTLYLEARPEAHLASLHFRP
                     DVDGAKVIVKAEFNQPLAGEGKLQLRARDQAFADITVPVPDGAGEFEFALPIPRPRLW
                     SLEDPFLYEVDAVLAAGGAQDRVSTYFGMRKISVIDLP"
     gene            complement(1115..2308)
                     /locus_tag="PKK20_11980"
     CDS             complement(1115..2308)
                     /locus_tag="PKK20_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00648.1"
                     /translation="MSDIRLCRLDDEASFAAVREFFEHDNPDLRLTRVSVDGSGHLRS
                     ECGGMRVFWVHRGRGEVFLPRGYRTQEGDGAPLPDQYRADALAPALAETLQLIESGLS
                     SLSPAARVPVAAILGRRREGSFVGDCLGELWRLDHVPEPWSTDERVVTALRSLYQVYR
                     QHGFSTKQVDSYEPVMEGDQIVACAQEAIPVRGCFQCLAMENVRQTASPVSTVRRLRF
                     LADTAGGCNPDFDPFRRLPLTWFYNYPGETGDGLNWVNSHVVNIPKETSPAHFHPPKA
                     VGGASGIPQREMYLVLDPIAYHLNTWGRSPSLIVFPDLRDLRRYEQHPLEPGLFIYIP
                     PGTGHRGLDVFVNVLTIPGFKPHNEYYLDRDIRDAAGGAAPFNENMLGAKNYERIEDL
                     LLTDP"
     gene            complement(2308..>3169)
                     /locus_tag="PKK20_11985"
     CDS             complement(2308..>3169)
                     /locus_tag="PKK20_11985"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00649.1"
                     /translation="NKARTAIGKRSEPLPIPETLDYDLWCGPARKEPIYRDRIQYDCS
                     FTWNMGDGESCNQGVHEIDVARWVLGETTLPRRVMSVGGRFVFDDAGDVPNTQIMCYD
                     YPSAPILYEVHNLRAGKDTQTMPSFRGSGVDTCVQCEGGYAMMHAGVVRDNRDKEIRK
                     FSGGEDHFGNFIRALRSGKREDLNAEILEGHLSTNICHAGNISYRLGRRASAVEVRAQ
                     IGDLPPFAEMFDRYLGHLKAHAIDPSESILGPWLECDPARECVKDNPRANEFVRGFYR
                     KPFEVPEVTA"
BASE COUNT          567 a         1041 c         1023 g          538 t
ORIGIN      
        1 ccggcaggtc gatgacactg atcttgcgca tgccgaaata agtcgacacg cggtcctgag
       61 ccccgccggc agccagcacc gcgtccacct cgtacaggaa cggatcttcc agcgaccaca
      121 agcgcggtcg gggaattggc agcgcaaact cgaactcgcc cgcaccgtcc ggcaccggca
      181 cggtgatgtc ggcgaatgcc tgatcgcgag cacgcaactg cagcttccct tcgcctgcca
      241 gcggctggtt gaattcggcc ttcacaatga ccttcgcccc gtccacatcc ggccgaaagt
      301 gcagggacgc cagatgggcc tccggacgcg cctcgaggta aagggtctgc cagattccct
      361 tggcggggcc gtagccctgt ttgccctcga gcttgaaggc atggggcgtg tcatcgacac
      421 gcaaaaccag gcgctgatcc tcgccccact tgacgaccgg cgtcagatcc cactcgaatg
      481 gcgtgtaccc gccgcggtgt tcgccgatcg actggccatc gagccacccg cgggtgagcc
      541 aatccgatgc cccgaccacc aggaagacgc gcttgccccg ccactccgcc ggcacgcgga
      601 tttgccggga ataccaaccg atgtctgcct cgttcccgac cccggacaag gctgagcccc
      661 agggaaacgg cacggtgatc ttcagcggga atgcctcctg actggcggcc caaccgcccg
      721 ccaggcccgc gttgtccttg tcgaatcgga actgccagga accgttcaga ttcaaccact
      781 ccgcgcgctc gaagtcgggc cgcgggtgct cgggcagcgg aattgcaggg gcgttggccg
      841 ccttcccggc ggcggccagg accagagcca gcccgattgc gcacgtggtc gtccgccgga
      901 gccgcaacgt ctgtgcccag aggtgaagga atggacttga catgtcctca gttcgccaga
      961 agcgcggccc gcctgcaagc cccgatgcaa agggtccgcg gcccggagcg ccaattcatg
     1021 aatacgaaaa ggccggagcg gagacggctc gcccccggcc aacgcgaatg aagacggtgt
     1081 ttctcggcga cggggccctg gcggctttgg ctggctaagg gtctgtcagc aacagatctt
     1141 caatgcgctc gtaattcttg gcgccgagca tgttctcgtt gaacggcgcc gcgccgcccg
     1201 cggcatcgcg gatatcgcgg tcgaggtagt actcgttgtg cggcttgaaa ccggggatgg
     1261 tcagcacgtt gacaaacacg tccaggccgc ggtgaccagt gcccggcggg atgtagatga
     1321 agaggcccgg ctccagtgga tgctgttcgt aacggcggag gtcccgcagg tcgggaaaca
     1381 cgatcaacga aggcgaacgg ccccaggtgt tcaggtggta ggcgatcgga tccaacacga
     1441 gatacatctc gcgctgcggg attccactgg cgccgccgac ggccttgggc ggatgaaaat
     1501 gcgccggcga ggtctccttg ggtatgttca ccacgtggct gttgacccag ttcagcccgt
     1561 cgcccgtctc gcccggatag ttgtagaacc aagtcagcgg caggcgacgg aagggatcaa
     1621 agtccgggtt gcagccgccc gcggtgtcgg ccagaaaccg caggcggcgg acggtcgata
     1681 cggggctggc cgtttgcctg acgttctcca tggccaggca ttggaagcag ccacgcaccg
     1741 ggatcgcctc ctgggcgcat gcgacaatct ggtcgccttc catcaccggt tcataggagt
     1801 cgacttgttt ggtcgagaag ccgtgctggc gatagacctg ataaagcgaa cgcagtgccg
     1861 tcacgacccg ttcgtcggtg gaccacggct ccggcacgtg gtcgagtctc cagagttcgc
     1921 ccaggcagtc gccaacgaac gagccttctc tgcggcggcc gaggatggcg gctacgggca
     1981 cgcgggcggc cggagacagc gacgaaagac cgctctcgat caactggagc gtctcggcca
     2041 gcgccggggc gagggcgtcg gcccggtatt ggtccggcag gggcgccccg tcgccttcct
     2101 gcgtgcgata cccgcggggc aggaacacct cacctcgccc ccggtggacc cagaatacgc
     2161 gcatgccgcc gcactcggac cgcaggtggc cgctcccatc cacggacacc cgcgtgagcc
     2221 gcaggtcggg gttatcgtgt tcgaaaaact cgcggacggc ggcgaaggac gcctcgtcat
     2281 ccaagcggca gaggcggatg tccgacatca ggccgtgacc tccggcactt caaacggctt
     2341 acggtagaag cctctgacaa actcgttggc ccgggggttg tccttgacgc attcgcgggc
     2401 cgggtcgcac tcgagccacg ggccgaggat cgattcgctc ggatcgatgg cgtgggcctt
     2461 gaggtgcccg aggtagcggt caaacatctc cgcgaacggg ggtaggtccc cgatctgcgc
     2521 ccgaacctcg acggcggagg cgcgtcgccc caagcgatac gagatgttgc ccgcgtggca
     2581 gatgttggtg gacaaatggc cctcgagaat ctctgcgttc aggtcctcgc gtttcccgct
     2641 ccgcaacgcg cggatgaaat tgccgaaatg atcctctccg ccgctgaact tcctgatctc
     2701 cttgtctcgg ttgtcacgca cgacccctgc gtgcatcatc gcgtaccctc cctcacactg
     2761 gacgcacgtg tcgacgccag agccgcggaa cgatggcatg gtctgggtgt ccttgccggc
     2821 ccgcagatta tgcacttcgt agagaatcgg cgccgacgga taatcgtagc acatgatctg
     2881 cgtgttcggc acgtcgcccg cgtcgtcgaa gacgaaccgt ccgccgacgc tcatgacgcg
     2941 ccgcggcaag gtcgtttccc ccagcaccca gcgggcgaca tcgatctcgt gaaccccctg
     3001 gttgcacgat tcgccgtcgc ccatgttcca ggtgaaactg cagtcgtatt gaatccggtc
     3061 gcggtagatc ggctctttgc gggccgggcc gcaccacagg tcgtagtcga gcgtctcggg
     3121 aatcggcaga ggctcgctgc gcttgccgat cgcagtccgc gccttgttg
//
LOCUS       DAOQRO010000377         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_35101_length_3150_cov_5.032310, whole genome shotgun sequence.
ACCESSION   DAOQRO010000377 DAOQRO010000000
VERSION     DAOQRO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35101_length_3150_cov_5.032310"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1659)
                     /locus_tag="PKK20_11990"
     CDS             complement(100..1659)
                     /locus_tag="PKK20_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00650.1"
                     /translation="MPTTLNPSRPPGGKYNTRHCPALDGVITPADCGAKRGSKLDCPD
                     DCPFFPFARAGFDLWVKVDNAWTAKALEYVERHMKLARFQEIVQRQRIPMPSEQLELE
                     CAVQNALYTVLFIQRDAAGSTLAQKWESENWAGLNNDEQVMMRCRLRGRPTVIEVQRI
                     VDAWCVECLDVLDPAAGPFRVLDRGLAARMVRFSRILTWLIPYPHFSRVSASAIEMPY
                     ALWPEWRATVEQRHQTARETRPDLTLKDFLAETIVETTMLVGELNDAHRARMLASLDV
                     EHCLTLFRYTCPRADLERVFDAKPDFERIDEGERSHADRTLVAYNWVGRGESATLAQG
                     TRGDAAAGTDSSAESVPVFGTARLFEGLVAFDTFSKRKQAFLRQIVERDFGSLLAFER
                     ETVKSLAAIMDERQRRGRILAFAEHLAFGDAAQAAPAAADPAPQNPDAAQKALAEQHL
                     RDYRGFLDAGIAALDGLSPRAAAADPEKRPRLIELMKQHLVILEQRNRDQGLNLTLDW
                     ALEELGLRELL"
     gene            complement(1776..2876)
                     /locus_tag="PKK20_11995"
     CDS             complement(1776..2876)
                     /locus_tag="PKK20_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNV00651.1"
                     /translation="MSISRRQFVARTAGATLSAAALSAVPALPADLPKGPDLEPSARK
                     LPRWRGFNLLEKFVKREGGNPPFLEQDFEWMSAWGFDFVRLPMSYLCWTDPTDWLRLQ
                     EDELKHIDEAIEFGKRHGVHVCLNFHRAPGYCVNPPPEPLDLWKDQKALDACAHHWTA
                     FARRYKGIPNRHISFDLFNEPDDQAEDNYARIAQRLVEAIRAEDPDRLIIADGLRWGN
                     KPVPALAPLGIAQSTRGYQPMRVSHHKASWVNGQNWPEPSWPLELGPGNTWDKERLRR
                     EQIAPWKALEKMGVGVHVGEWGAYQFTPHAVALAWMKDNLDLWKEAGWGWALWNFRGS
                     FGILDSARSDVKYEDWNGHRLDRAMLELLRQG"
     gene            complement(2890..>3150)
                     /locus_tag="PKK20_12000"
     CDS             complement(2890..>3150)
                     /locus_tag="PKK20_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV00652.1"
                     /translation="AKTHYSIAIHQQPGYPWGKSARSVGPLTNAETNAACCISLPMFP
                     ELRQDEVDYVIAKVKEWDAMFAAQAPAGKGPAQRESACACCK"
BASE COUNT          523 a          945 c         1092 g          590 t
ORIGIN      
        1 catcgaggcc taggcgccgg tcgataatgg ccagggcgcc ccagggacgg tcgtggcatg
       61 agccgaacac gcaggctggg gagcgaggag catggggagt cataacaact cgcgaagtcc
      121 cagttcctcg agggcccagt cgagtgtgag gttcaagcct tgatcgcgat tccgttgttc
      181 aaggatgacc aggtgctgtt tcatcaactc gatcaaccgc ggacgctttt ccggatctgc
      241 cgccgccgcg cgcgggctca atccgtccag ggcagcgatg ccggcgtcga ggaacccccg
      301 ataatcacga agatgctgtt ccgccagcgc tttttgggcc gcatccggat tctgaggggc
      361 gggatcggct gcggcgggcg ccgcttgggc ggcgtcgccg aaagccagat gttccgcgaa
      421 ggccagaatg cgtccgcgtc gttggcgctc gtccatgatg gcggccaagc tctttaccgt
      481 ttcccgttcg aacgcgagca acgatccgaa atcgcgctcg acgatttggc gaaggaaggc
      541 ctgtttgcgt tttgaaaacg tgtcgaatgc caccaatccc tcgaaaagtc gcgcggttcc
      601 gaacaccggc accgattcag ccgaggagtc cgtccccgcg gccgcatcgc cccgtgtccc
      661 ctgggcgagc gtggcggatt cgccccggcc cacccagttg taggcgacga gtgtgcgatc
      721 ggcgtgggat cgctcgcctt catcaatccg ctcgaaatcc ggtttggcgt cgaagacacg
      781 ctcgagatcg gctcgtgggc aggtgtagcg gaagagggtc aagcagtgct cgacatcgag
      841 actggcgagc attcgggcgc gatgggcgtc gttcagttcc cccacgagca tggtggtttc
      901 gacgatggtc tcggccagga agtccttgag ggtcaggtcc ggacgcgttt ctcgagcggt
      961 ttgatggcgt tgctcgacgg tggcacgcca ttcgggccag agggcgtaag gcatttcgat
     1021 ggcgctggct gatacgcggg agaagtgcgg atacgggatc agccaggtga gaatgcggct
     1081 gaagcggacc attcgagcgg ccagcccgcg atcgagaacg cggaacggcc cggccgcggg
     1141 gtcgaggaca tccaggcact cgacgcacca cgcgtcgacg atgcgctgga cctcgatcac
     1201 tgtcgggcgc ccgcggagtc ggcagcgcat catcacctgc tcatcgttgt tcaggcccgc
     1261 ccagttttcg ctctcccact tctgggcgag ggtactgccc gcggcgtccc gctggatgaa
     1321 caggaccgtg tagagcgcgt tctggacagc gcattcgagt tccagttgct cgctcggcat
     1381 cgggatgcgc tggcgctgga cgatctcctg gaaccgcgcc agtttcatgt gtctttcaac
     1441 gtactcgagc gctttggccg tccaggcatt gtccaccttc acccagagat cgaaaccggc
     1501 gcgggcgaag ggaaaaaagg ggcaatcgtc ggggcaatcg agcttgctgc cgcgtttggc
     1561 tccgcagtcc gccggggtga tcaccccatc gagcgcgggg caatgccggg tgttgtactt
     1621 gccgcccggg gggcggcttg gattcaatgt tgtgggcatg cgaaacgaga caacagaatt
     1681 cctcagactt gggcgggaag caaacgacgc agatggcccg atgccgcccc gccccggagg
     1741 gctggggggc tttggctcgc tggcttcgtt gctcctcagc cctgccgcag cagttcgagc
     1801 atggcgcggt cgagccggtg gccgttccaa tcctcgtact tcacgtcgga acgtgcgctg
     1861 tcgaggatgc cgaacgagcc gcggaagttc cacagggccc agccccagcc ggcttccttc
     1921 cagaggtcga ggttgtcctt catccaggcc aaggcgacgg catggggtgt gaactgatag
     1981 gcgccccatt cgccgacatg cacgccgacg cccattttct cgagcgcctt ccagggcgcg
     2041 atctgctccc ggcgcaggcg ttccttgtcc caagtgttgc ccggcccgag ttcgagcggc
     2101 cagctcggtt cgggccagtt ctggccgttg acccaactcg ccttgtgatg gctgacgcgc
     2161 atcggctggt agccgcgggt gctttgggcg atgcccagcg gagccagggc aggcacgggc
     2221 ttgttccccc acctcaagcc gtcggcgatg atcaggcggt ccgggtcttc ggcccggatg
     2281 gcctcgacaa gccgttgcgc aatgcgggcg taattgtcct cagcctggtc gtcgggctcg
     2341 ttgaagaggt cgaagctgat gtggcggttg gggatgcctt tgtagcggcg agcgaatgcg
     2401 gtccagtggt gggcgcaggc gtcgagcgct ttctggtcct tccacaaatc gagcggttcc
     2461 ggcggcgggt tgacgcaata gccgggggcg cggtggaagt tgaggcagac atggacgccg
     2521 tggcgtttgc cgaactcgat cgcctcgtcg atatgcttga gttcgtcctc ctgcaatcgc
     2581 agccaatccg tcggatcggt ccaacaaaga tacgacatgg gcagacggac gaagtcgaag
     2641 ccccaggcgg acatccactc gaaatcctgt tccaggaagg gcgggttgcc cccttcgcgc
     2701 ttgacgaact tctcgagcag gttgaagccg cgccagcggg gcagcttcct ggctgagggc
     2761 tccaggtccg ggcccttggg gaggtccgcg ggcaacgcgg gaacggcgct gagcgctgcg
     2821 gcgctcagcg tagccccggc cgtgcgcgcg acgaattggc ggcgggagat gctcatgggt
     2881 gtgtgaatgc tacttgcagc aggcgcaggc ggattcgcgc tgagcggggc ccttgcccgc
     2941 aggggcctgg gcggcgaaca tcgcgtccca ctctttgacc ttcgcaataa cgtaatcgac
     3001 ttcgtcctgg cggagttcgg ggaacatggg gaggctgatg cagcaggccg cgttggtctc
     3061 ggcgttggtg agcgggccga cgctgcgggc ggatttgccc cacgggtagc ctggctgctg
     3121 atggatggcg atcgagtaat gggtcttggc
//
LOCUS       DAOQRO010000378         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_35637_length_3120_cov_3.803915, whole genome shotgun sequence.
ACCESSION   DAOQRO010000378 DAOQRO010000000
VERSION     DAOQRO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35637_length_3120_cov_3.803915"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKK20_12005"
     CDS             complement(<1..632)
                     /locus_tag="PKK20_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNV00653.1"
                     /translation="MPPPSRRKRALPRIPRRDAWARTRGLGHAWPKPPSRGILTALEQ
                     LNAPVDRFEFERRLLCRVTRIAGVDEAGRGPLAGPVVAAAVVLPESFIRGGLSADLEG
                     LNDSKQLTARQRDRCFEALTHTPGVASAVSAVGIEVIDAINILRATHQAMAEALAQLE
                     PRPEHVLVDGLPVKSLAYPQTALVKGDARSYSIAAASIVAKVTRDRVMLE"
     gene            complement(580..1035)
                     /gene="rplS"
                     /locus_tag="PKK20_12010"
     CDS             complement(580..1035)
                     /gene="rplS"
                     /locus_tag="PKK20_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNV00654.1"
                     /translation="MNQALLDRIEAAHLRKRPLRFEVGDTVRVHTKVVEGDRERIQVF
                     AGVVIGRRGHGINETFTVRRISYGEGVERVFPSHSPRIERIEIERKGSVRRAKLTYLR
                     KRIGKSAVAVKEKEHHTADATAAAPTKQSEAVNAAAESPQAGAAADTKT"
     gene            complement(1061..1753)
                     /gene="trmD"
                     /locus_tag="PKK20_12015"
     CDS             complement(1061..1753)
                     /gene="trmD"
                     /locus_tag="PKK20_12015"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNV00655.1"
                     /translation="MKIDVLTLFPGMFAGPLDQSIVGRAREQGVLDLRIHNLRDFTHN
                     RHKTVDDKPFGGGPGMLMKPEPIFEAVECLRQPDTRVILLCPAGRLFQQCIARELAVL
                     PHLLLICGSYEGVDERVREHLVDDELSIGDYVLTNGALPAMVIVDVVARLLPGALGDD
                     SSARDESFSAGWLEYPQYTRPAQFRGWKVPDVLLSGHHAAISLWRAEQAQQRTRQRRP
                     DLMGPPGSEPGD"
     gene            complement(1819..2067)
                     /locus_tag="PKK20_12020"
     CDS             complement(1819..2067)
                     /locus_tag="PKK20_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNV00656.1"
                     /translation="MQAWLEFVVKGLTDRPDAVTVMPQERDGFTVYELRLHPTDVGKI
                     IGRQGATIHALRALLQVGSARQGQRCSLELVEEVPPKD"
     gene            complement(2082..2342)
                     /gene="rpsP"
                     /locus_tag="PKK20_12025"
     CDS             complement(2082..2342)
                     /gene="rpsP"
                     /locus_tag="PKK20_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNV00657.1"
                     /translation="MAVRIRLKRIGTTNAPAYRIVVADGRSPRDGRFIEEIGSYLPQK
                     REDNVTLALERAEYWLSKGARPTETVASFIKRARKSAPAPQG"
     gene            complement(2491..>3120)
                     /locus_tag="PKK20_12030"
     CDS             complement(2491..>3120)
                     /locus_tag="PKK20_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNV00658.1"
                     /translation="GRGEFGRIAGLPALTDGGYDDLAARLFQSRRFPSWGYEVVNGAT
                     TVWERWDSYTKEHGFNGASGNQNAAMNSFSHYAFGAVMEWAFRNLAGIDTDGPGYSRI
                     VIRPCPPMPDSNPEQTPIHWVKAEYQSIRGLIRSEWRRHADAFELRVSIPGNTTATVY
                     VPALNADSIRESGQPLAGAQGVRLLRKQGDRIVVAVESGDYRFTSSLSH"
BASE COUNT          530 a         1047 c          955 g          588 t
ORIGIN      
        1 aattcgagca tgacgcggtc tcgggtgact ttggcaacga tgctggcagc ggcaatcgaa
       61 tagcttcggg cgtcgccttt gacaagggcg gtttgggggt aggcgagcga tttcaccggc
      121 aacccgtcca ccaggacatg ctcgggtctg ggctcgagtt gagcaagcgc ttcggccatc
      181 gcctggtgcg ttgcccgcag gatattgatc gcgtcgatga cctcgatccc gaccgcacta
      241 acggcgctgg ccacgcctgg ggtgtgtgtc agcgcctcaa agcaacggtc tcgctgccgc
      301 gccgtgagct gtttcgagtc gtttaaaccc tccaagtccg ccgaaagccc gccgcggata
      361 aagctctccg gcaacacaac cgccgctgcc accaccggac ccgcgagcgg accgcgcccc
      421 gcttcgtcca caccggcaat ccgggtgacg cggcagagaa ggcggcgctc gaactcgaag
      481 cgatcgaccg gggcattcaa ctgctcgagg gcagtcagga ttccgcggct gggcggcttc
      541 ggccaggcgt ggccgaggcc gcgcgtccgg gcccaggcat cacgtcttgg tatccgcggc
      601 agcgcccgct tgcggcgact cggcggcggc attgacggct tcggattgct tcgtcggcgc
      661 agccgcggtg gcgtcggccg tgtggtgttc cttctccttg accgcgacgg cgctcttgcc
      721 gatgcgcttg cgcaggtaag tcagtttggc gcggcggacg gagcctttac gttcgatctc
      781 gatgcgctcg attcgaggag aatgcgaggg gaacacccgc tcgacgcctt cgccgtagct
      841 gatgcgtcgg acggtgaagg tctcgttgat cccgtgtccg cggcgtccga tcacgacacc
      901 ggcaaacacc tggatgcgtt ctctgtcgcc ctcgacgacc ttggtgtgaa cgcgcacggt
      961 gtcgccgact tcaaatctca gagggcgttt gcgcaaatga gccgcctcga ttctgtccag
     1021 tagtgcctga ttcataaatg gtcgtgctat cgttttctct ctaatctccc ggctcgcttc
     1081 ccggcgggcc catcagatcg ggccgccgct gccgcgtcct ctgctgcgcc tgttcagccc
     1141 gccagaggct gatcgcggcg tggtgtccgg acagcaacac gtccggcacc ttccatcccc
     1201 tgaattgcgc gggccgcgtg tactgcggat actcgagcca gcccgcgctg aaggattcat
     1261 cccgggcact cgagtcgtct ccgagtgcgc ccggcaagag ccgcgccaca acgtccacga
     1321 tgaccatagc cgggagcgcg ccattggtca gcacgtaatc gccgatcgac aactcgtcgt
     1381 ccaccagatg ctcgcgcacc cgctcgtcca cgccctcgta actgccgcag atcagcagca
     1441 gatgggggag aacggccagt tcgcgggcga tgcactgctg aaagagccgt ccggccgggc
     1501 agagcaggat gacccgagtg tccggctgtc gcaagcactc aaccgcttca aagatcggct
     1561 cgggcttcat cagcatgccc ggcccgcccc caaacggctt gtcatccacc gttttgtggc
     1621 ggttgtgggt aaagtcacgc aggttgtgta tccgcaaatc caacacgccc tgctcccgag
     1681 cccgacccac gatgctctga tcgaggggac ccgcaaacat tcctggaaac agggtaagca
     1741 catcgatctt cataccgccc ccgatcgttg gcctcgagcc ccatctcgca acacccgcct
     1801 ggtcaggccg ggatgggatc agtctttggg cgggacttcc tcgaccagtt cgaggctgca
     1861 gcgctggccc tggcgggcgc ttcccacctg gagcagagcg cgcagcgcat gaatggtcgc
     1921 accctggcgt ccgatgatct tcccaacatc ggtcggatgc agacgaagtt cgtacaccgt
     1981 gaagccatcc cgctcctgcg gcatcacagt gacggcatcg gggcgatccg tcaaaccctt
     2041 gaccacgaat tcaagccagg cctgcatgag accttccccg ctcaaccttg gggcgccggc
     2101 gcgctcttcc gggcccgctt gatgaagctg gcaaccgtct cggtcggacg cgcccctttg
     2161 ctcagccagt actcggcccg ctccaaagcg agcgtcacgt tatcctcccg cttctgaggc
     2221 aggtacgatc cgatttcctc gatgaaacgc ccgtcgcgcg gactgcgccc gtcggcgacc
     2281 acaatgcggt aagccggggc gttggtcgtc ccgatccgct tcaagcgaat tctgactgcc
     2341 ataatattgt gtatgatttt tcgcccggtc ccgcctcgca tttcgctggc ggccccggcg
     2401 tcatccgcat tggcggactc aaaagagcgg ggagcctaga ccgcaatcgg cgcgattggc
     2461 aagacctgaa ttcgcctccc cgtgctgcct tcaatgggac aggctcgacg tgaatcggta
     2521 atccccagat tccaccgcca caacgattcg atcgccttgc ttgcgcaaca gccgcactcc
     2581 ctgggcgccg gccaacggct gcccgctctc gcggatcgag tccgcgttca acgccggcac
     2641 atacacggtc gcagtcgtgt tcccggggat gctcactctc agttcgaagg cgtcggcgtg
     2701 gcgacgccat tccgaccgga tgagcccgcg aatactctgg tactcggctt tgacccaatg
     2761 aatcggtgtt tgttcagggt tgctgtcggg catcggcggg cacgggcgaa tcacaatccg
     2821 gctgtaaccc ggcccatcgg tgtcgatgcc ggcaaggttg cggaaggccc attccatgac
     2881 ggcgccgaag gcataatggc tgaagctgtt catcgcggca ttctgattgc cgctggcgcc
     2941 gttgaagccg tgttcttttg tgtagctgtc ccagcgctcc cagacggtcg tggccccgtt
     3001 gaccacctcg tagccccacg aggggaaccg ccggctctgg aaaaggcgcg ccgccaggtc
     3061 gtcgtagccg ccgtcagtca gggccgggag tcccgcgatg cggccaaact cgccgcggcc
//
LOCUS       DAOQRO010000379         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_35703_length_3117_cov_4.306009, whole genome shotgun sequence.
ACCESSION   DAOQRO010000379 DAOQRO010000000
VERSION     DAOQRO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35703_length_3117_cov_4.306009"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PKK20_12035"
     CDS             complement(<1..479)
                     /locus_tag="PKK20_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNV00659.1"
                     /translation="MDRLTFATVWLGGCSGCHMSFLDLDEWLVDLADMVEIVYSPIID
                     RKEYPENVAVCLIEGAVANEDNLEQVKLIRARTRLLISFGDCAVTGNVTAMRNPLRTA
                     EPVLQRAYLDTANLRPRIPQARGIVPPLLDQVLPVHEVVPVDYFLPGCPPPADRIRT"
     gene            complement(467..1183)
                     /gene="hoxU"
                     /locus_tag="PKK20_12040"
     CDS             complement(467..1183)
                     /gene="hoxU"
                     /locus_tag="PKK20_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HNV00660.1"
                     /translation="MPVKTLTIDGKPVSAVEGQTVLEAAQDAGISIPTLCHLDGVSDV
                     GACRLCLVEVAGVSRLLPACVTKVTEEMEVRTESERLKRYRRMILELLFAERNHVCAV
                     CVSNGRCELQNLAVAQGMDHVRFAYRFPPYEVDITHRMFGMDHNRCVLCSRCVRVCWF
                     IEGAATKNVAGRGADARIITDLSQPWGESETCTSCGKCVQACPTGAKFHKGSTVAEME
                     RDRSRLEYIVTAREKKQWIV"
     gene            complement(1240..2889)
                     /gene="nuoF"
                     /locus_tag="PKK20_12045"
     CDS             complement(1240..2889)
                     /gene="nuoF"
                     /locus_tag="PKK20_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNV00661.1"
                     /translation="MDLADLLEIKEKELRTRKPIRLRCCLAAGCVSSNGQAVKESLDK
                     AVQEAGLADEVEVRQVGCMRLCCEGPLVAVDPAGILYQKVAPEDAASIVAALKGGETQ
                     VKRGDLNHPFFTKQMSIVLENSGEIDPERIESYIAADGYQALNDALVEMKPKEVIDAI
                     LQSGLRGRGGAGYPTGLKWATVAKMQGSKKYVICNADEGDPGAFMDRSVLESDPHRLL
                     EGMAIAAYAVGADQGFIYVRAEYPLAISRLHTAIKQAKQHGLLGSGVLEAAFTFNVEI
                     RIGAGAFVCGEETALMASIEGERGTPRPRPPFPAERGLYAGPTLINNVETLANVPSIV
                     RKGADWFAGIGTEKSKGTKVFALAGKITNTGLIEVPMGTSLRTIVEEMGAGAPDGNQV
                     KAVQTGGPSGGCLPASQLDTPVDYESLQQLGSIMGSGGMIVMDQTTNMVDVARFFMEF
                     CMDESCGKCTPCRAGTVQLHALLDRILRRQATARDLELLEELCDLVKHTSLCGLGQTA
                     PNPVLSTLRFFRNEYLELLQPEIGAPAAFGTPPKPDVNPTG"
     gene            complement(2876..>3117)
                     /locus_tag="PKK20_12050"
     CDS             complement(2876..>3117)
                     /locus_tag="PKK20_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV00662.1"
                     /translation="CMGTACYVKGADRVLAAIEQHAGIKAGETTSDNQLSLLTARCIG
                     ACGIAPAVVYDGAVTARQSAEDALERVKGWTNGSR"
BASE COUNT          558 a         1096 c          889 g          574 t
ORIGIN      
        1 aaggtgcgaa tgcggtccgc cggaggcgga cacccaggca ggaagtagtc cacgggcacc
       61 acctcgtgca ccgggagcac ctgatcgagc agcggcggca caatgccccg cgcctgcgga
      121 atgcggggac gcaggttggc ggtgtcgaga taggcccgct gcaacaccgg ctccgccgtc
      181 cggagcgggt tgcgcatggc cgtcacattc cccgtgacgg cacaatcccc gaacgagatc
      241 aacaggcgag tccgggcgcg gatcagcttc acctgctcga ggttgtcctc gttagccacg
      301 gctccttcga tgaggcaaac cgccacgttc tccgggtact ctttgcggtc gatgatcggg
      361 ctgtagacaa tctcgaccat gtcggcgagg tccaccagcc actcgtcgag gtccagaaac
      421 gacatgtggc agccggaaca gccgcccagc cagacagtcg cgaaagtcag acgatccatt
      481 gcttcttctc ccgtgcggtg acgatgtact cgaggcgcga gcgatcccgt tccatctccg
      541 ccaccgtcga acctttgtga aacttggccc ccgtgggaca cgcctgaacg cacttgccgc
      601 aggaggtgca cgtctccgat tccccccacg gttgactcag atcggtgatg atgcgcgcgt
      661 ccgcgccgcg gccagccacg ttcttcgtcg cagcgccctc gatgaaccag cacacccgga
      721 cacaccggct gcaaagcacg caccggttat gatccatgcc gaacatccga tgcgtgatgt
      781 cgacctcgta gggcgggaac cgataggcga atcgcacatg gtccattccc tgcgcgaccg
      841 ccagattctg cagctcgcag cgcccgttgg atacgcagac ggcgcagacg tggttgcgtt
      901 cggcaaaaag cagttcgaga atcatccggc gataccgctt cagccgctcg ctctccgtcc
      961 gcacctccat ctcctcggtc accttggtga cgcaggcggg cagaagccgc gacacgccgg
     1021 cgacctcgac caaacaaagc cgacaggcgc cgacatccga caccccatcc aggtgacaga
     1081 gcgtcgggat ggaaatcccc gcgtcttgag ccgcttccag cacggtctgg ccctcgaccg
     1141 cgctgaccgg tttgccatca atcgtgagtg ttttgacagg cataatgcga tttcccgttg
     1201 ctcaacctaa tcgttccaca cagcgcgcct cttcgggatt caacccgtcg gattcacgtc
     1261 gggctttgga ggggtgccga aagcggccgg cgcgccgatc tcgggttgca gcaactcgag
     1321 atactcgttc ctgaaaaagc gcagcgtgct cagcaccggg ttgggcgccg tctgccccag
     1381 gccgcacagg cttgtgtgct tcaccagatc gcagagttct tcgagcagtt ccaggtcgcg
     1441 ggccgtggcc tgacggcgca gaatgcggtc caggagtgcg tgcaactgca ccgtgccggc
     1501 ccggcacggg gtgcacttgc cgcacgattc gtccatgcag aactccatga agaagcgcgc
     1561 cacgtcgacc atattcgtgg tctgatccat gacgatcatg cctcccgatc ccatgatcga
     1621 tcccagttgc tgcagcgact cgtaatccac cggcgtgtcc aactgggacg ccggcaagca
     1681 accgcccgat gggccgcccg tctgaaccgc cttcacctga ttgccgtcgg gcgcgcccgc
     1741 ccccatctcc tcgacaatcg tgcgtagcga cgtgcccatc ggcacctcga tcagaccggt
     1801 gttggtgatc ttgcccgcca gagcgaacac cttggtgccc ttgctctttt cagtcccgat
     1861 gcccgcaaac cagtccgccc ctttccggac gatcgacggc acgttggcga gtgtctcgac
     1921 gttgttgatc aacgtcggac cggcatacaa gccgcgctcc gccgggaacg gcggacgcgg
     1981 ccggggtgtg ccccgctcgc cctcgatcga cgccatcagg gctgtctcct cgccgcagac
     2041 gaaggccccg gcgccaatcc ggatttcgac gttgaatgtg aacgcagctt cgagcacgcc
     2101 actgcccagc aacccatgct gcttggcctg tttgatggcg gtgtgcagcc ggctgatggc
     2161 cagcgggtac tcggcccgga cataaatgaa accctggtcg gcgccgacgg cataggcggc
     2221 gatggccatc ccttcgagca ggcgatgggg atcgctctcg aggacgcttc ggtccatgaa
     2281 cgctcccgga tcgccctcgt ctgcattgca aatcacatac ttcttggagc cctgcatctt
     2341 ggcgacggtc gcccacttca gccccgtcgg atagcccgcc cctcctcgcc cccgcaaacc
     2401 actctgcagg atcgcatcga ttacctcctt cggcttcatc tccaccagcg cgtcgttgag
     2461 cgcctggtag ccgtcggcgg caatgtacga ttcaatccgt tcggggtcga tttcaccgct
     2521 gttctcaagc acgatcgaca tctgcttggt gaaaaacgga tggttcagat cgccccgctt
     2581 gacttgggtc tcgccccctt tcagggcggc gacgatcgac gccgcatctt ccggggcgac
     2641 cttctgatag aggatgccgg ccggatccac tgccaccagc ggcccctcgc aacacaatcg
     2701 catgcagcca acctgccgca cctccacctc gtccgccaac cccgcctcct gcaccgcttt
     2761 gtcgagactc tctttgaccg cctggccatt ggacgacacg cagcccgccg ccagacagca
     2821 ccgcagccgg atcggcttgc gtgtccgcag ttccttctcc ttgatttcca gcagatcagc
     2881 gagatccatt ggtccatcct ttcacgcgtt caagcgcgtc ttccgcgctc tgccgcgccg
     2941 tcaccgcgcc atcgtacacc acggccgggg caattccgca cgccccgatg caacgcgccg
     3001 tcagcagcga caactgattg tcggatgtcg tctcgccagc cttgatcccg gcatgttgct
     3061 cgatcgccgc caggacccga tcggcccctt tcacatagca ggccgttccc atgcaga
//
LOCUS       DAOQRO010000380         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_35744_length_3114_cov_4.522720, whole genome shotgun sequence.
ACCESSION   DAOQRO010000380 DAOQRO010000000
VERSION     DAOQRO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35744_length_3114_cov_4.522720"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PKK20_12055"
     CDS             complement(<1..1164)
                     /locus_tag="PKK20_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00663.1"
                     /translation="MKACNRREFAKTLAFASAAAGLSARASNVNDRVRLGFIGLGNRG
                     DQVLDAFLAQPDAQIVAVCDISQPYLDFAAKKIGTQPRQHKDYRKLLEQKDVDAVVIS
                     TPDHWHALQTIHACEAGKDVYVEKPLSLRVAEGRAMATAVRRYGRVCQVGIHRRSVDF
                     CRQAAEFVRAGGIGKVTVARAFHVQNEWPKGIGNPPDADPPADFDWDAWLGPAPKVPY
                     NKNRTFYRFRWFYDYSGGQVTNFGVHYIDFIQWALGQTAPMRVTALGGRFAGIDDNRE
                     IPDTLEAVWHYPGDTLVTFSQFNATAAPGSLPGCEVELRGTKGTLYLFGNRFEVVADA
                     ITPNEFPARTPTDRAYERRWRQGEQRMIENRKVTGDGDTAHHARNFLHCVKSRE"
     gene            complement(1230..>3114)
                     /locus_tag="PKK20_12060"
     CDS             complement(1230..>3114)
                     /locus_tag="PKK20_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00664.1"
                     /translation="RYERMDLAFERADWIATPAAEALIRNNRPLLAYLAGKPARFTSK
                     DHNFLPGEIVEKQMIVINNSRESVTCQCQWSCNLPQAVSARQQVAVPAGQQERLVLRC
                     PLPLGVPRGQYDLVARFQFGTGETQEDRILLHVLPAEPQPIGADSGRRESLPATTPDS
                     GSRATDPLPATIPESESAKPRVALFDPKGQTQASLKRLGWDPQPVQAETDLSAFDILI
                     VGKEALSVDGPAPDIHRVRDGLKVIVFEQTSEALEQRLGFRVQEYGLRQLFPRVPDHP
                     VLTGLATEHLRDWRGEATILPPRLKLDMRPRHGPTVQWCGLPVTRLWRCGNQGNVASV
                     LIEKPARGDFLPILDGGYSLQYSPLLEHRDNQGMVLFCQVDVTGRTEDEPAAEILVRN
                     LLRYVSAWKPPARRSLVYAGDPLGRSHLESVGIAVSTFDGHSLAPGALLVVGRGGGAA
                     LAPHQAAIADWLQAGGRVLGVGLGQEDLDAWLPVAVTTRKAEHIAAFFEPFAHQACFA
                     GVGPADVHNRDPRDVPLVTDGARPVGNGVLALHDRAPIVLCQLAPWEFIPTAQPNLRR
                     THRRLSCLLSRLLGNLGVRASTPVLDRFHRPVARASEERWLDGLYLDRPEEWDDPYRF
                     FRW"
BASE COUNT          542 a          942 c         1064 g          566 t
ORIGIN      
        1 ctctcggctc ttgacgcaat ggaggaaatt gcgcgcgtgg tgagccgtat ccccgtcgcc
       61 ggtcaccttg cggttttcga tcatccgttg ttcgccctgg cgccagcgac gttcgtaggc
      121 gcggtcggtt ggagtgcgcg cggggaattc gttcggggtg atggcatccg ccacgacctc
      181 gaaacggttg ccaaagaggt agagcgtgcc tttggtccca cgaagttcga cctcacatcc
      241 gggcaggctt cccggagccg cggtggcatt gaactgtgag aaggtcacca gtgtgtcgcc
      301 cgggtagtgc cagacggcct cgagcgtgtc gggaatctcg cggttgtcat cgatgcctgc
      361 gaaacgcccg cccagcgccg tcacgcgcat cggggcggtt tgaccgagcg cccactgaat
      421 gaaatcgatg taatggaccc cgaagttcgt cacctgccct ccggagtagt cgtagaacca
      481 gcggaaccgg taaaacgtgc ggttcttgtt gtaggggacc ttgggcgcag ggccgagcca
      541 ggcgtcccag tcgaagtcag cgggcgggtc ggcatctggc gggttgccga ttcccttggg
      601 ccattcgttc tggacgtgga aggcgcgtgc gaccgtgacc ttgccgatgc cgccggcgcg
      661 cacgaattcg gcggcttgcc tgcagaaatc gaccgatcgg cggtggatgc ccacctggca
      721 tacgcgaccg tagcgccgga ctgccgtggc catggcccga ccctccgcca cccgcagcga
      781 aagcggtttc tcgacgtaca cgtccttacc cgcctcacac gcgtggatgg tctgcagcgc
      841 atgccagtgg tccggggtcg agatgaccac ggcatcgaca tccttctgct cgagcagctt
      901 tcggtagtcc ttgtgctgtc ggggctgagt gccgatcttc tttgcggcaa aatcgaggta
      961 cggctggctg atgtcgcaga ctgcgacaat ctgggcgtcg ggttgcgcga gaaacgcatc
     1021 gagaacctgg tcgccgcggt tgcccagtcc gatgaacccg aggcgcaccc ggtcgtttac
     1081 gttcgaggcg cgggcggaga ggccggcagc ggcgctggcg aaggcaagtg tttttgcgaa
     1141 ttcacgacga ttacaggctt tcatatcgac ctcgagtcta tccgctctcg gttcccttgg
     1201 ccagcctgag aaagagcggg cgtgccgctc taccaacgga agaagcgata aggatcgtcc
     1261 cattcctcgg ggcgatcgag atagaggccg tcgagccagc gttcttccga ggctcgggcg
     1321 accgggcggt gaaagcggtc aagcaccggc gtcgaggcgc gcactccgag attgcccagg
     1381 aggcgtgaga gaaggcacga gagtcggcgg tgagtgcgtc tgaggttggg ttgggcggtg
     1441 ggaatgaact cccaaggcgc aagctggcag agaacaatcg gcgcgcggtc gtgcagggca
     1501 agcactccat tgccgacggg cctggctccg tcggtcacaa gcggcacatc gcgcgggtcg
     1561 cggttatgca cgtcggccgg tccgacaccc gcaaagcacg cttgatgagc gaagggttcg
     1621 aagaacgcag cgatgtgctc ggccttcctg gtggtgacgg caacgggcag ccaggcgtcc
     1681 agatcctcct gtccgagccc cactcccaga acccgcccac ccgcctggag ccaatcggcg
     1741 attgcggctt ggtgtggcgc caacgccgct ccgccgccgc gaccgacgac gagcagcgcg
     1801 ccgggcgcaa gcgagtgtcc gtcgaaggtc gatacggcga tgccgacgga ttccaggtgg
     1861 cttctcccga gcggatcgcc ggcatagacc agagatcgtc gcgcaggggg tttccaggct
     1921 gacacatagc ggaggagatt tcggacgagg atttcagccg ccggttcgtc ctcggtgcgt
     1981 ccggtgacat cgacctgaca aaacagcacc ataccctgat tgtcccgatg ctcgagcagc
     2041 gggctgtact ggaggctgta gccgccatcg aggatcggca ggaaatcgcc ccgcgcgggt
     2101 ttctcgatca acacggaggc cacgtttccc tggttcccac agcgccagag ccgagtgacg
     2161 ggcaggccgc accattggac ggtcgggccg tggcgcggac gcatatcgag cttgagtcgc
     2221 ggcggcagaa tggtcgcctc gccgcgccaa tcgcgcaagt gctccgtcgc gagccccgtg
     2281 agcacaggat ggtccggcac ccgcgggaag agctgtcgga gtccgtactc ctgaactcga
     2341 aacccaaggc gctgttcgag ggcctcgctc gtctgctcga acacgattac tttcagccca
     2401 tcgcggaccc ggtggatgtc gggcgccggg ccatcgacac tcaatgcctc tttgcccaca
     2461 atgaggatgt cgaaggcaga caaatcagtt tcagcctgga caggctgcgg gtcccagccc
     2521 aatcgcttca gagaagcctg agtctggcct ttggggtcga acagggcgac gcgcggtttc
     2581 gccgattcgg attctggtat ggttgcaggc aaaggatcgg tcgcacggct gccggaatcc
     2641 ggtgtggttg cgggcagaga ctctcggcgg cccgaatccg caccgattgg ctgaggctct
     2701 gccggaagca cgtgcaacag gatgcggtct tcctgtgtct cgcccgtgcc gaattgaaat
     2761 cgggcgacca gatcgtattg gccgcgcggc acgcctaggg gcagggggca gcgcaggaca
     2821 agccgctctt gctggcctgc ggggacggcg acctgctggc gtgccgacac ggcttgcggg
     2881 aggttgcacg accactggca ctggcatgtg acgctctcgc gggaattgtt gatgacgatc
     2941 atctgttttt cgacgatctc gccggggagg aagttgtggt ctttgctggt gaagcgggcc
     3001 ggtttgccgg ccaggtatgc cagcagcgga cggttgttgc ggatgagggc ctccgcggcc
     3061 ggcgtcgcga tccagtccgc gcgttcgaag gccagatcca tccgttcgta ccgt
//
LOCUS       DAOQRO010000381         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36053_length_3097_cov_5.075608, whole genome shotgun sequence.
ACCESSION   DAOQRO010000381 DAOQRO010000000
VERSION     DAOQRO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36053_length_3097_cov_5.075608"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="PKK20_12065"
     CDS             complement(<1..1996)
                     /locus_tag="PKK20_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-agarase"
                     /protein_id="HNV00665.1"
                     /translation="MCGALLVTSVNAADLRLVDFSMVQVGGLLALSDSQVTNIRSSSG
                     AALRVATGHRETWPGVTLKAPDGRWDLTAFAQVVAAVRNPGSKSVTVNCRVDNPGADG
                     TSHCITRSVTVGAGATNLLQVELRRAGDSTMGGKLFGLRGYPVASGGQGTIDPANITQ
                     ILVFVSNPQTDLVFEVGEFHATGRYTPPTAWVSDADPFFPFIDALGQYRHKAWPGKTP
                     SPASLASQRDAEARSLARVPGIRGWNRYGGWAAGPQLEATGFFRVQKHGGKWWLVDPE
                     GRLFWSHGIDCVRMLDATPIDERADWFVDFPGDQADFVEFITSAYALKGHYAGRSPKC
                     FSFAGANLKRKYGAEWREVYPDIVHRRLRAWGLNTIANWSDEGIRLMRRTPYTDTIGS
                     SHARRIEGSEGYWGKFPDVFDPAFREGLRRQMASKAGKSAGDPWCIGYFSDNEMGWGD
                     EISLATGALASPADQPAKRVLLDDLRAKYGEIGKLNAAWGSSHESWDALAASPAKADR
                     QKAGVDLRAFYTKAAEQYFSTIREVIKEVAPNQLYLGCRFAWGNDRAAAAAAKFCDVV
                     SYNLYQRSVADFRFNGNADVPLIIGEFHFGALDRGLFHTGLVPVGNQAERARAYQDYV
                     RGALRHPQFVGCHWFQYQDEPTIGRVLDEENYQIGFIDIAD"
     gene            complement(2220..2963)
                     /locus_tag="PKK20_12070"
     CDS             complement(2220..2963)
                     /locus_tag="PKK20_12070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNV00666.1"
                     /translation="MQTEFIFSPLVPTRDKDASLTVGTARVPLQFVRHRRARRYILRL
                     TAEGVARVTIPRGGSTSEGRRFAERQIEWIERQTRRRQEAALRPTGLFIGSRILFRGE
                     PILLSAGAADGAMVVRFADQAVRIGNLFDDVRPRLEQHLWSMAKAELPPRVQALAAHH
                     GFAVRGVRVRNQRTRWGSCSRHGAISLNWRLIQVPAHVRDYIILHELAHLREMNHSAR
                     FWSEVAKLCPGYREAEQWLRKQGKSLAAF"
BASE COUNT          634 a         1077 c          833 g          553 t
ORIGIN      
        1 tgtcggcaat gtcgatgaat ccaatctggt agttctcctc gtcgagcacg cgcccgattg
       61 tcggctcgtc ctggtactgg aaccaatgac agcccacaaa ctgcggatgt cgcagcgccc
      121 ctcgcacgta gtcctgatag gcccgcgcgc gctcggcctg gttgcccaca ggcaccaagc
      181 cggtgtgaaa aagcccccga tccagagcgc caaagtgaaa ctcgccaatg atcaacggca
      241 cgtcggcatt cccgttgaac cggaaatccg ccacgctgcg ctggtagaga ttgtagctga
      301 ccacgtcgca gaacttcgcc gccgccgccg ccgcgcggtc gttcccccac gcaaaccggc
      361 agccgagata gagctggttc ggggcgactt ccttgatcac ttcgcggatg gtgctgaagt
      421 actgctccgc ggcctttgtg tagaaagccc gcagatcaac gcccgccttc tgccggtcgg
      481 ccttggcagg actcgcggcc agggcgtccc acgactcatg ggaactgccc cacgcggcat
      541 tgagcttccc gatctcgccg tacttggccc gcaggtcgtc gagcagcacg cgcttggccg
      601 gttgatccgc cggagacgcc agtgcccccg ttgccagaga aatctcgtcg ccccacccca
      661 tctcgttgtc cgaaaaatac ccgatgcacc agggatcgcc cgcgctcttg cccgccttcg
      721 acgccatctg ccgccgcaaa ccctctcgga atgcgggatc aaacacatcc gggaatttgc
      781 cccagtaccc ctcgctcccc tcgatccgcc gagcgtgcga cgacccgatc gtgtccgtgt
      841 acggcgtccg gcgcatcagg cgaatgccct catccgacca gttcgcgatc gtgttcaggc
      901 cccacgcccg caggcgtcga tgcacaatgt cgggataaac ctccctccac tcggctccgt
      961 atttccgttt gaggttcgcc ccggcaaaag aaaaacactt gggcgaacgc ccggcgtagt
     1021 gccccttcaa cgcataggcc gacgtgatga actcgacaaa atcggcctga tcgcccggaa
     1081 agtcgacaaa ccaatccgcc cgctcgtcga tcggcgtcgc atcgagcatc cgcacacagt
     1141 cgattccatg ggaccagaat agccgaccct ccggatcgac aagccaccat ttgcctccat
     1201 gcttctgaac acgaaaaaaa cccgtcgcct cgagttgcgg tcccgccgcc caaccgccat
     1261 accggttcca cccccgaatg ccgggcaccc tggccagcga ccgcgcctcg gcatcccgct
     1321 ggcttgccag gctcgccggc gatggcgtct tgccgggcca ggccttgtgc cggtattgcc
     1381 caagtgcgtc aatgaacgga aagaacggat cggcatcgct gacccaagcc gtcggcggcg
     1441 tgtaacggcc cgtcgcgtga aactcaccca cctcgaaaac cagatccgtc tgcggattgc
     1501 tcacaaagac aagaatctgc gtgatgttcg ccgggtcgat cgtcccctgc cccccggacg
     1561 ccaccgggta accccgcaaa ccgaagagct tgccgcccat cgtgctgtcg ccggcccgtc
     1621 gcagttcgac ctgcagaaga ttcgtcgccc cagccccgac cgtcaccgag cgcgtaatgc
     1681 aatggctcgt cccgtcggcg ccaggattgt ccactcgaca attgaccgta acgctcttcg
     1741 atccggggtt ccgcacagcg gcgacgacct gggcaaacgc cgtgagatcc catcgcccat
     1801 caggcgcctt gagagtcacg cccggccacg tttcacggtg accagtggca acccgcaacg
     1861 cggcgcccga gctgctccgg atgttcgtga cttgcgagtc gctcagagcc agcaacccgc
     1921 cgacctggac catcgagaaa tcgaccagac gcaagtcggc ggcattgacc gaggtgacga
     1981 gaagcgcacc acacatcaac gcggatggcc caacacagat atgcagtaac ctggagaaaa
     2041 acatgttcaa tcccatagaa accgatgtat taacgttagt gttatcgtaa ataatatcat
     2101 ctcatgacgt cctatgttca tccgaaaaga ccccatcggc ctcggagggc cgagtctcaa
     2161 aaggctgcag accctcgcgg tgcgcttccc tgctagagcg ctcaccttga cagactcctt
     2221 caaaacgctg caagcgattt cccttgcttc ctgagccatt gctcggcttc ccgatatccg
     2281 ggacacaact tggcgacttc cgaccagaac ctcgcggagt ggttcatctc ccgcagatgc
     2341 gccagttcgt gcaaaatgat gtagtcgcgc acatgcgccg ggacctggat cagcctccaa
     2401 ttcaacgaaa tcgccccatg ccgcgaacac gatccccaac gcgtccgctg atttcgcacc
     2461 cgcacgccgc gcacagcgaa gccgtggtgc gccgccagag cctggacgcg cggcggcaat
     2521 tccgcctttg ccatcgacca aagatgctgt tcgagccgcg gacgcacgtc gtcaaacagg
     2581 tttccgattc ggacggcctg atcggcaaat cgaaccacca tcgcgccatc cgcagccccc
     2641 gccgacagaa ggatcggttc tccgcgaaac aggattcggc tgccgatgaa caaccctgtt
     2701 ggacgcaacg cggcctcctg ccgccgccgc gtctgccttt caatccactc gatctgtcgc
     2761 tcggcgaagc ggcgcccttc ggatgtcgat ccgccgcgcg ggattgtcac acgcgccacg
     2821 ccttccgccg tcaacctgag aatgtagcgc cgggcgcgcc gatgccgcac gaattgcagc
     2881 ggcactcgtg ccgtgccaac cgtcaaagaa gcgtctttgt ctctggtggg aaccaacggc
     2941 gaaaagataa actcggtttg cacagggtct ccaacccgtt ccgctcctgc ttttcggtga
     3001 cgagagttac agaagcgtcc gtatctgtca aagggaagcg atcgtccctg atcctcgcct
     3061 aattttctga aagtgtgaaa aaagtgtgtg tacatgc
//
LOCUS       DAOQRO010000382         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36065_length_3097_cov_3.469099, whole genome shotgun sequence.
ACCESSION   DAOQRO010000382 DAOQRO010000000
VERSION     DAOQRO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36065_length_3097_cov_3.469099"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKK20_12075"
     CDS             <1..1039
                     /locus_tag="PKK20_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNV00667.1"
                     /translation="ALLDAVDPAMLDTLLARLSSAGIEKIDYVVSHHTEQDHSGGIPR
                     ILAQYPEAQVLASDKAKGLLTDHLGLSEDRLRVVQDGETIDLGGLTLEFIYFPWVHWP
                     ETMLTWLPERRVLFTCDLFGSHLATSDVLAHGDPRILPAAKRYYAEIMMPFRGFIEKN
                     LDRVTALDPAMIAPSHGPVYDEPGLILAAYRDWVSGPPKSLVLLPYISMHGSTRILVD
                     HFVDACARRNVGVEQINLVDADVGKLAVLMVDAATIVLGTPTVLGGPHPRVAYAALLA
                     NLLKPKARHLSVIGSMGWGGKAVEQLAAAVSSLKLELLPPVLCKGLPKPADIEAVEAL
                     AEAIAGKHAGL"
     gene            complement(1153..>3097)
                     /gene="lptD"
                     /locus_tag="PKK20_12080"
     CDS             complement(1153..>3097)
                     /gene="lptD"
                     /locus_tag="PKK20_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNV00668.1"
                     /translation="VRLQRGAEVWTGQTLRYDFASRQIQASDFRTGRSPYFAAGHGLG
                     SDLTNKVHTATNGFVTTDDFAEPGYRVRAKSLTFKPGEYIEARGATLYVGKVPVMYLP
                     RYRRNLNRESNHWYALPGYRSLYGAYLLTTYNWSIATNAAASLHLDYRTERGVAGGPD
                     VEYDLGRAGDGRLSAYYAHDEDAGVGELSEPIPADRWRVGFSHSAFVHTNLSVKAAVN
                     AESDPYFRHDFLENEYRKDIMPKSFVEVDRQWSNFSLDLVAQPQINDHLQTVERLPDL
                     KLTGLRQQLGISPFYYESESSLAYMRHREGDFAPATNYAALRGDTYHQLLLPWNFFGW
                     LNVMPRAGGRYTYYGAEHDTLLDQDSQDRWVFNTGAEVSTKMSRLWRNARSRVFDVSG
                     LRHIVEPSVNYVYVPTPTSRPLDLPQFDYEWPGLEMMPIEYPDYNSIDAIDSQNVLRF
                     GLRNRLQTKRRDRVENLLHWALFMDWRLRPRADQETFGDLYSDIDFTPRTWLVLNSEL
                     RYDINTTSWRMANHTVTLRPNDVWQWKVGHRYLSEIPGFGPNSGNNLVLSSLHFRLNE
                     DWSFRTSQHFEARTGTMQEQYYTVFRDFRSWTGALTLRLRDRTETLRQKEDWTVAFQV
                     SLKAFPRSSTPQSRDDPIYLLGY"
BASE COUNT          546 a          951 c         1016 g          584 t
ORIGIN      
        1 cgcgcttctc gatgcggtcg atcccgccat gctcgatacc ctgctggccc gcctatcgag
       61 cgcgggtatc gagaagatcg attacgtcgt ctcgcaccac accgagcagg atcactcggg
      121 cggcattccg cgaattctcg cccagtatcc cgaggcgcag gtgcttgcca gcgacaaggc
      181 gaaaggactg ctcaccgatc atctgggtct ttcggaggac cggctgcgcg tggtgcagga
      241 cggcgagacg atcgatctcg gaggtctgac cctcgagttc atctacttcc cgtgggttca
      301 ctggcccgag acgatgctga catggctgcc ggaacggcgc gttctcttca cctgcgacct
      361 gttcggttcg catctggcca ccagcgatgt tctcgcccat ggggacccgc gcatcctccc
      421 cgccgcgaaa cgttactatg ccgagatcat gatgccgttc cgcggtttca tcgagaagaa
      481 cctggaccgc gtgactgcgc tcgatcccgc gatgatcgcg ccgagccacg ggccggtgta
      541 cgacgagccc ggcctgatac tcgctgcgta tcgggattgg gtgtcgggac cgccgaagag
      601 cctggtgctt ctgccctaca tctcgatgca tggttccacg cgcattctcg tcgaccattt
      661 cgtcgacgcc tgcgcgcggc gcaacgtggg cgtcgagcaa atcaatctcg tcgatgccga
      721 cgtcgggaag ctggcggtgc tgatggtgga cgccgcgacc atcgtgctcg gcacccccac
      781 tgtgctgggc gggccgcatc cgagggtcgc ctacgccgcg ctcctggcca acctgctcaa
      841 gcccaaggcc cgccaccttt cggtcatcgg ctcgatgggg tggggcggca aggcggtcga
      901 gcaactcgcc gcggctgtct cgagcctcaa actcgaattg ctccctcccg ttttgtgcaa
      961 ggggctgccc aagccggctg acatcgaggc ggtcgaggcg cttgccgaag caatcgccgg
     1021 gaaacacgca gggctgtgat cgggtgccag cccgcgaatc tcgtgcccca caggcacgcg
     1081 gttgttcggg cgctcaaggc ctctgctccg ccgctcacag cggccagatc agcaggagca
     1141 tcgggatgct gatcaatacc ctaggagata gatgggatcg tcacggctct gaggggtgct
     1201 cgagcgtgga aacgccttga gcgagacctg gaacgcgacc gtccagtctt ccttttgtcg
     1261 cagggtctcg gtccggtcgc gcaggcgcag cgtgagcgcg cccgtccaac tccgaaagtc
     1321 gcggaacacg gtgtaatact gctcctgcat cgtgccggtg cgggcctcga agtgctgcga
     1381 ggtccggaag gaccagtcct cgttgagccg gaaatggagg ctgctcagga cgaggttgtt
     1441 gcccgagtta gggccgaatc cggggatttc gcttagatag cgatggccga ccttccactg
     1501 ccagacgtcg ttggggcgga gggtgacggt gtgattggcc atgcgccacg acgtggtgtt
     1561 gatgtcgtag cggagttcgg agttcaagac cagccacgtg cgcggtgtga aatcgatgtc
     1621 cgaatagaga tcgccaaagg tctcctgatc ggcgcgcggg cggagccgcc agtccatgaa
     1681 cagcgcccag tgcaggaggt tttcgacccg gtcgcggcgt ttggtctgga gcctgttgcg
     1741 cagaccgaag cggagcacgt tctggctgtc gatggcgtcg atggaattgt agtccggata
     1801 ctcgatcggc atcatctcga ggccgggcca ttcgtaatcg aactggggca gatcgagcgg
     1861 acggctggtc ggagtgggga cgtagacgta gttgaccgac ggttcgacga tgtggcgcag
     1921 gcccgacacg tcgaagacgc ggctgcgcgc gttgcgccag aggcgggaca ttttggtcga
     1981 gacttcggcg cctgtgttga acacccagcg atcctgcgaa tcctggtcaa gcagagtgtc
     2041 gtgttccgcg ccataatagg tgtaacgccc gcccgcgcgc ggcatgacgt tcagccagcc
     2101 gaagaagttc cacggcaaca gaagctgatg gtaggtgtcg ccgcggaggg cggcatagtt
     2161 ggtggcgggc gcaaagtcgc cctcgcggtg gcgcatgtag gccagcgaac tctcgctctc
     2221 gtagtagaag ggcgaaatcc ccagttgctg acgcaggccg gtcagtttca gatccggcag
     2281 gcgctcgacc gtctgcagat gatcgttgat ctgcggctgc gcaaccaggt ccagactgaa
     2341 attcgaccac tgccggtcga cctcgacaaa cgacttgggc atgatgtcct tgcggtactc
     2401 gttctcgaga aaatcgtgcc ggaagtaggg atccgactcc gcgttgaccg cggccttcac
     2461 gctcaggttg gtgtgaacga atgccgagtg cgagaacccg acgcgccagc ggtcggcggg
     2521 gatcggctcg ctcaactcgc ccacgccagc atcctcgtcg tgggcatagt acgcgctgag
     2581 gcgtccgtcg cccgctcgac caaggtcata ttccacatcc ggcccgcccg ccaccccgcg
     2641 ctctgtgcga tagtccagat gcagagacgc ggctgcattt gtggcaatcg accagttgta
     2701 ggtcgtcagc aggtacgccc cgtacagact ccggtaaccg ggcagggcgt accagtggtt
     2761 ggattcccgg ttcagattgc ggcggtaccg cggaaggtac atgaccggca ctttgcccac
     2821 gtagagcgtc gccccgcgcg cctcgatgta ctccccaggc ttgaacgtca ggctcttggc
     2881 ccggacccga taacccggct ccgcgaagtc gtcggtcgtc acgaaaccgt ttgtggcggt
     2941 gtggactttg ttggtcagat cgctgcccaa gccatgcccg gccgcgaagt aaggcgatcg
     3001 gccggttcga aagtcggacg cttgaatctg tcgagacgcg aaatcgtagc gcagtgtctg
     3061 gccggtccat acctccgcac cgcgctggag gcggaca
//
LOCUS       DAOQRO010000383         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36290_length_3086_cov_4.449687, whole genome shotgun sequence.
ACCESSION   DAOQRO010000383 DAOQRO010000000
VERSION     DAOQRO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36290_length_3086_cov_4.449687"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3086)
                     /locus_tag="PKK20_12085"
     CDS             complement(<1..>3086)
                     /locus_tag="PKK20_12085"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00669.1"
                     /translation="GSTNVVNNGDFENGQTGWVFSGNHSTSTVDTTGAASGTRCLHVR
                     GQGDGDTGPNTLRNTLRTSLANNSTVTIRAKVRWLAGWPQILFRLHGNGLELAANMAV
                     PPNLGTPGLPNSRLVANAGPAIYDVAHAPPLPAANEPVRVTCRVSDPDRLAQVQLRYR
                     IDPSSTLSTVIMRDDGAQGDERAGDGLFSAILPAQSAGTLAAFRITATDAGTPAASAT
                     FPARAPAEECLVRWGDAIPFGTFAHYHLWFTRATANARQNALDNTYRDSTLVCSDHRV
                     IYNTGFRDKGSPYHGGAGDIAVTTPRDEPLLGTFDRVFASTGNGGSESTGIRSQLAAW
                     YAQQLGIPYLHADYMRLYFNGSLFRSDIMEDLEQPNHNYAERWFPTGDEGDLYKIAVW
                     FEFGDDNRNFQATGATAQRFTTLNNEYKLARYRWNFQRRSNDGDASNYGQFLDLVTAL
                     NDSSPSYAGRVSSQADMEQWMRVFCFDYAMGNWDAWTYNVGQNMYLYRPLGQRWVLMP
                     WDIDFVFGLGDGTSASLRGGGQDPTMSRAYSNAEFLRMNWRAYQDTINGPFLPANFQP
                     QIDARRAILLKNNVTGISAPGSITSWINGRRTYISNQLNAVDSKTFKIGDDFQSTTPT
                     VTLNGTAPIAVATIELNGVPYPVAWSTIRNFSVTVPLTQKTNLLTFVGKDLRGNPVPG
                     ATNTIEVRYTGAIERMEDFVVINEVHYDAAPSEPASTFIELFNRSSTTLFDLTGCVLQ
                     GVGYTFPKGALIAPRSFLVLARNRPGFEAVYGRGVPVFDLFPGSLDNDGERLALIRPG
                     GAGTPDLTVSDLRYWNRLPWPTNAAGLGPSLQLVDAIHGSWRVGNWAAPPTNHIDRAT
                     PGRANALAQTLAPFPPVWLNEVQPSNVAGPRDGAGDRDPWIELYNAGAETVDLSLLYL
                     TDAYTDLTRWRFPDGTTLGAGQFLVVWADGEPGESRPGEPHTSFRLAPDTGTIALCRL
                     QGSPTAPAVIDYIEYNQLSAGRSLGSIPDGEPRARRPFVEVTPGAPNNPSLPAVPV"
BASE COUNT          489 a          815 c         1226 g          556 t
ORIGIN      
        1 ggactggcac ggcgggcaag gacgggttgt tgggagcgcc cggggttacc tcgacgaacg
       61 ggcgtcgtgc gcgtggctca ccgtccggaa tagacccgag gctgcgtccg gcggacaact
      121 ggttgtactc gatgtagtcg atcaccgccg gcgcggtggg ggacccctgc aagcggcaga
      181 gggcgatcgt tccggtgtcg ggcgccaggc ggaagctggt gtggggctcg ccgggacggg
      241 attcgcctgg ttcgccgtcg gcccatacga cgaggaactg gcctgcgccg agggtggtgc
      301 cgtcggggaa acgccagcgg gtgaggtcgg tgtaggcgtc tgtcaaataa agaagtgaaa
      361 ggtcgaccgt ctccgcgccg gcgttgtaca actcgatcca agggtcgcgg tcgccggcgc
      421 cgtcgcgggg gccggcgaca ttgctgggct ggacttcgtt gagccagacc ggcgggaagg
      481 gcgcgagcgt ctgggcgagg gcgttggcgc ggcctggggt ggcgcggtcg atgtgattgg
      541 tgggtggtgc ggcccagttg cccacgcgcc aggagccatg gatcgcatcg acgagctgca
      601 aggacggtcc taggccggcg gcgttcgtgg gccaggggag gcggttccag tagcgcagat
      661 cgctcacggt caagtcgggt gtcccggcgc cgccgggtcg gatgagtgcc agtcgttcgc
      721 catcgttgtc gagcgagcct gggaacagat cgaacaccgg cacgccgcgg ccatagaccg
      781 cctcgaaccc cgggcgattg cgcgccagga cgaggaatga ccgcggcgcg atgagggcgc
      841 ccttcgggaa ggtgtagccg acgccctgaa gcacgcagcc ggtgaggtcg aaaagcgtcg
      901 tcgacgagcg gttgaagagt tcgatgaaag tcgaggccgg ctcgctgggc gcggcatcgt
      961 aatggacttc attgatgacg acgaagtctt ccatccgctc gatggcgcca gtgtagcgga
     1021 cctcgatggt gtttgtggcg ccggggacag ggttgccgcg gaggtctttg ccgacgaacg
     1081 tcagcaagtt ggtcttctgg gtcagaggca cggtcacgct gaagttgcgg atagtgctcc
     1141 aggcgaccgg gtaaggaacg ccgttgagct cgatggtggc gacggcgatc ggcgcggtgc
     1201 cgttcagggt gacggtggga gtcgtcgact ggaaatcgtc gccgatctta aacgttttgg
     1261 agtcgacggc gttgagctga ttgctgatgt aggttcggcg gccgttgatc caggaggtga
     1321 tggagccagg ggcgctgatg ccggtgacat tgttcttgag gaggattgcg cggcgggcgt
     1381 cgatttgggg ttgaaagttg gcggggagga acgggccgtt gatggtgtcc tggtaagcgc
     1441 gccagttcat gcggaggaac tccgcattcg agtaagcgcg gctcatcgtg gggtcctggc
     1501 cgccaccgcg cagggaagcg ctggtgccgt cgccgagccc gaagacgaaa tcgatgtccc
     1561 agggcatgag gacccaacgc tggccgagcg ggcggtagag gtacatgttc tggccgacgt
     1621 tgtaggtcca cgcgtcccag ttccccatgg cgtagtcgaa acagaacacc cgcatccatt
     1681 gttccatgtc ggcctggctc gacacgcggc cggcgtagct tggcgacgag tcgttgagcg
     1741 cggtgaccag gtcgaggaac tgcccgtagt tggaggcgtc gccatcgttc gagcgacgct
     1801 gaaagttcca gcggtagcgg gcgagtttgt attcgttgtt gagggtggtg aagcgttgcg
     1861 cggtggcgcc ggtggcttgg aagttgcggt tgtcgtcgcc gaactcgaac cagacggcga
     1921 tcttgtacaa gtcgccttca tcgcccgtgg ggaaccagcg ttcggcgtaa ttgtgattgg
     1981 gctgttcgag gtcctccatg atatcggatc ggaacaagga cccgttgaag tagagcctca
     2041 tgtagtcggc atggaggtaa gggatgccca gttgctgggc gtaccaggcg gccagttggc
     2101 tgcggatgcc ggtggattcg gagccaccgt tgccggtcga ggcgaacacg cggtcgaacg
     2161 tgcccagcag cggctcgtcg cgaggtgtgg tgacggcaat gtcgcccgcg ccgccgtgat
     2221 aggggctgcc cttgtcgcgg aagccggtgt tgtagatgac ccggtggtcg ctgcagacca
     2281 gggtggaatc gcgataggtg ttgtcgaggg cgttctgtcg ggcgttggcg gtggctcggg
     2341 tgaaccagag gtgatagtgc gcaaaggtgc cgaaggggat agcgtcaccc caccgcacca
     2401 ggcattcctc ggccggggcg cgggcgggga acgtggcgga ggcggccggc gtgccggcat
     2461 cggtggcggt gatgcggaag gcggccaagg tgccggcgga ctgggcaggc aggatcgccg
     2521 agaagagacc gtctcccgcc cgttcgtcgc cctgggcgcc gtcgtcacgc atgatgacgg
     2581 tggacagcgt cgaggaaggg tcgatgcggt agcgcagttg cacctgggcc aggcggtcgg
     2641 ggtctgagac gcggcaggtg acgcggacgg gttcgttggc ggcggggagg ggcggggcgt
     2701 gggcgacgtc gtagatggcg gggccggcgt tggcgacgag gcggctattg ggcaatcccg
     2761 gagtgccgag gtttggcggg acggccatat tggccgccag ttcgaggccg ttgccgtgga
     2821 gtcggaagag gatttgcggc caaccggcca gccaccggac cttggcgcgg atggtgacgg
     2881 tgctgttgtt ggcaagagac gttcgcaggg tgttgcggag cgtgttgggt ccggtatcgc
     2941 cgtctccctg gccgcggacg tggaggcagc gggtgccgct ggcggcacct gtggtgtcca
     3001 cggtcgaggt gctgtgattg ccgctgaaga cccagccggt ctggccgttc tcgaagtcgc
     3061 cgttgttgac gacattggtg ctgccg
//
LOCUS       DAOQRO010000384         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36554_length_3072_cov_3.331787, whole genome shotgun sequence.
ACCESSION   DAOQRO010000384 DAOQRO010000000
VERSION     DAOQRO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36554_length_3072_cov_3.331787"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKK20_12090"
     CDS             complement(<1..688)
                     /locus_tag="PKK20_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00670.1"
                     /translation="MNGHIPQTHRRTHALATVLAGALTFLCSADPPATNLAPRKVQVA
                     AGDLEGVFHVAADGIRLDQLRDRSTTTILATNAAPLFALTITNATTREHRIMTASEGW
                     GEVDLRVDRHRLRLRWGAPLSEGLAGIAVSAIAALDQQSSALRWTLHVDNRSTAWAVR
                     QTVFPQLAVPELAHDAAVLFPRGPGEVQRGLWRRPFTYRGNYPDGWCAMQFLAAYREG
                     ESPVGVYLGFH"
     gene            complement(685..1218)
                     /locus_tag="PKK20_12095"
     CDS             complement(685..1218)
                     /locus_tag="PKK20_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNV00671.1"
                     /translation="MSGQAETSTAEAQAIRQARLTRHGGFRFDSAAWRAKVQTRTHAI
                     QLAEYYGLITHMDEQIGRILTALEAAGHARRTLIVYAADNGLALGIVYPKIGHAQLFD
                     LQNDPHEITNLVDHSKHSVQVARLRLLMRQWQARVGDALEIPDSHKQPDPIDLTGRPR
                     VPDQWQPEWIVKKYFDL"
     gene            1287..2648
                     /locus_tag="PKK20_12100"
     CDS             1287..2648
                     /locus_tag="PKK20_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00672.1"
                     /translation="MTRRRFLRRSALAAGSTLVLVGPAWRGVFGADSAVRRAVSPNDK
                     LNIAIIGVGGRGGGNLADVSSENIVVLCDVHNAPVLAAAQKFPQAKRYRDLRRVFDDA
                     RDFDAVVVSTCEHTHAFAVLPALQLGKHVYCEKPLTYNVWEAHLIIEAARKAKVATQM
                     GTQIHATDNYRRVVELVQTGAIGPVNEVHVWVGRAWGWQSEEEAKAHGDIVSVRDRPS
                     GSSPIPQGLDWDLWIGPAPYRPFHEVYWPGPKWYRWWDFGNGTMSDLGSHWNDLPWWA
                     LELDHPRTVEAEGPPPHPEIAPASMHALYTYGARGDLPPVTLHWHQGTMRPKPYQEGT
                     IPKWDSGVLFVGAKGMLLADYGKHVLLPEDQYKNFKAPAPFIPSSKGHHREWIDACKT
                     GEPTTCNFLYAGPLTIANHLGNVAFRAAARLDWNPIALEAKNCPAATPFIRREYRPGW
                     RLG"
     gene            2714..>3072
                     /locus_tag="PKK20_12105"
     CDS             2714..>3072
                     /locus_tag="PKK20_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00673.1"
                     /translation="MNKTSLFSWTAVLAGVFLFIYSSPAAAPDAAPAPTHAPIPWDQI
                     GAKAGADYRGDGLRVTAQGESVKLNCMFQRLEGEATPEGLWLTSTVTNQVQDRFRVVA
                     TALGRTTSNPLSRPDGHP"
BASE COUNT          568 a          956 c          990 g          558 t
ORIGIN      
        1 catggaaacc gaggtagaca ccgacgggac tctccccttc ccggtaggcc gccaaaaact
       61 gcatcgcgca ccagccgtcc ggataatttc cccggtacgt gaaggggcgt cgccagagcc
      121 cgcgctgcac ttcgccgggg cccctcggaa agagcacggc tgcgtcatgg gccagctcgg
      181 gcacggcgag ttgggggaag accgtctggc gaaccgccca agcggtagac cggttgtcga
      241 catgcagagt ccagcgcagg gcgctcgatt gttgatccag agcggcgatg gcgctgacgg
      301 cgatgccggc cagtccctcg ctgagggggg caccccagcg gagtctgaga cggtggcggt
      361 cgacgcgaag gtcaacctca ccccagccct cggatgctgt catgatccgg tgctcccggg
      421 tggttgcgtt ggtgatcgtc aacgcgaaaa gcggcgcggc attcgtagcc agaatcgttg
      481 ttgttgagcg atcgcggagt tgatcgagcc ggattccgtc ggcggcgaca tgaaacacgc
      541 cctcgagatc gcccgcggcg acttggacct ttcggggagc cagattcgtg gcaggtggat
      601 cggcgctgca cagaaacgtg agcgcgccgg cgaggaccgt ggccagcgca tgggttctcc
      661 gatgtgtttg cgggatgtga ccattcataa atcgaagtac ttctttacga tccattccgg
      721 ctgccactgg tccggcaccc gcggacgccc ggtcagatcg atcggatcgg gttgcttgtg
      781 ggaatcgggg atttcaagcg catccccgac gcgcgcctgc cattgtctca taaggaggcg
      841 gaggcgcgca acctggacgg aatgcttgga atggtcaacg aggttggtga tttcgtgggg
      901 atcgttttgc aggtcgaaca actgggcatg cccgattttg gggtagacga tgcccagggc
      961 cagaccgttg tcggcggcat aaacgataag cgtgcggcgg gcgtgcccag cggcttcgag
     1021 tgccgtcagg atgcgtccga tctgttcatc catgtgcgtg atgagcccgt agtattcagc
     1081 aagttgaatc gcgtgtgttc gtgtctggac cttagcgcgc catgccgcgg agtcgaagcg
     1141 aaaaccgccg tgacgcgtaa gtcgcgcttg gcgaatggcc tgtgcttccg ctgtcgatgt
     1201 ttccgcttgt ccagacatcc catcgggtgt agtcgaacgg catgaccaaa tgcactgcat
     1261 cccgctcctc taagtcttcg catcctgtga cacgccgccg gttcctgcgc cgatccgccc
     1321 tggcggccgg cagcacgctg gttctggtcg gaccggcctg gcgcggcgtg ttcggcgcgg
     1381 actcggccgt ccgccgggcc gtcagtccga acgacaagtt gaacatcgcg atcatcggtg
     1441 tgggcgggcg tggcggaggc aacctggctg acgtgagcag cgagaacatt gtcgtgttgt
     1501 gcgacgtgca caacgcgccg gtcctggccg ccgcgcagaa gtttccccaa gccaagcgct
     1561 atcgcgacct gcggcgcgtg ttcgacgacg cacgggactt cgacgccgtg gttgtgagca
     1621 catgcgagca cacgcatgcg tttgctgtcc tgcccgccct gcaactgggc aagcatgtct
     1681 attgcgagaa accgctcacc tacaatgtgt gggaagccca cctgattatt gaagccgccc
     1741 gcaaggccaa ggtggccacg cagatgggaa cccaaatcca tgccaccgac aactaccggc
     1801 gcgtggtcga gttggtgcag accggcgcaa tcgggcccgt gaacgaggtc catgtctggg
     1861 tcgggcgcgc ctggggttgg caaagcgaag aagaggccaa ggcccatggc gacattgttt
     1921 cggtgcgcga tcggcccagc ggatcatctc caatcccgca gggactcgac tgggacttgt
     1981 ggatcggccc cgcgccctac cgtccgttcc acgaggtcta ttggcccggc cccaagtggt
     2041 accgctggtg ggactttggc aacggcacaa tgtccgatct gggcagtcac tggaacgatc
     2101 tgccttggtg ggcgctcgaa ctcgatcatc ctcggaccgt cgaggccgag ggtccgccgc
     2161 cacacccgga gatcgcgccg gcatcgatgc atgccctcta cacgtacggc gctcgcggag
     2221 acctgccgcc cgtgactttg cactggcacc agggcacgat gaggccaaaa ccgtaccaag
     2281 agggcacgat tccgaagtgg gatagcggcg tgctgtttgt cggcgcgaaa gggatgctgc
     2341 tggccgacta tggcaaacat gtgctgctgc cagaagacca gtacaagaac ttcaaagcgc
     2401 cggctccgtt catcccgtcc tcgaaaggcc accaccggga atggatcgac gcctgcaaga
     2461 ccggcgagcc gacgacctgc aatttccttt acgcggggcc gctgactatc gccaatcacc
     2521 tcggcaatgt cgcctttcgc gccgccgccc gactcgactg gaatccaatc gccctcgagg
     2581 caaagaactg ccccgccgcc acgcctttta tccgacgcga atacaggccg ggctggcgcc
     2641 tcgggtaacc gcgtttagcc cggttatttc ccgcgcttcg cgaggaaccg taacattcaa
     2701 accatcaaac cccatgaaca aaacctcatt gttctcctgg accgccgtct tggccggcgt
     2761 gtttttgttc atctacagtt ccccggcggc ggcgccggac gcggcgccag cgcccaccca
     2821 cgcgccgatt ccgtgggatc agatcggggc gaaggctgga gcggattacc gcggcgatgg
     2881 tctgcgggta acggcgcagg gcgagagtgt caaactgaac tgcatgttcc aacggctgga
     2941 aggagaagcg acgccggaag ggttgtggct cacctcgacg gtgaccaatc aagtccagga
     3001 ccgcttccgc gtggtggcca cggcactggg ccggacgacg tccaatcccc tctcccgacc
     3061 tgacggtcac cc
//
LOCUS       DAOQRO010000385         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36659_length_3066_cov_5.176021, whole genome shotgun sequence.
ACCESSION   DAOQRO010000385 DAOQRO010000000
VERSION     DAOQRO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36659_length_3066_cov_5.176021"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PKK20_12110"
     CDS             complement(<1..1331)
                     /locus_tag="PKK20_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00674.1"
                     /translation="MTHTIRSAAWVVALSVAGAGIAWGFVYETDHELIGTGDLDGNRR
                     QDVVIFDRATGKYRVGYQSADGLFNWVNYRVSGLKDPTWLTLGRLVSEDKDALAFTAA
                     DANLVHVVEAANPGSPGKPVAIEFESLGPGALVAVDIGGPGNTPLDDLAVSSVYDDPN
                     KIVLFRNDAGSFAVNAQPEIKATWVHPNRLALKRGGPLYVTGLWSGEDSDAFRVSDFA
                     SGSEVIVAEAGGLPKGSDYVVGFFRGAPLADVVTYKKGEKSIQFHPLTEAGGKLGVGE
                     AKTYELASPIMLLVAVPKAQGAGLLAVYGKGESAEVLALDAAGAPGAVQSITATSGDL
                     IFGAVGLDESFLVFTGADYMKFATDARAFKPEGAAYAAGELVKLASMQDNDDATVPDI
                     HERIVTILEKEAIRSANDMKLYTNTIPGTQVSYVMVPIPGGEFSMGSPDSEA"
     gene            complement(1648..1968)
                     /locus_tag="PKK20_12115"
     CDS             complement(1648..1968)
                     /locus_tag="PKK20_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNV00675.1"
                     /translation="MITTTTHTIEGRLVQEYLGIATGEAIIGANLFRDVFASVRDLVG
                     GRSASYERVLADARATALREMEEVAVARGADAVLGVDLDYEVLGAQNGMLMVTASGTA
                     VKLR"
     gene            2689..>3066
                     /locus_tag="PKK20_12120"
     CDS             2689..>3066
                     /locus_tag="PKK20_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNV00676.1"
                     /translation="MSTVSPAAAQERVLGADISYWNCGSSSTGISQANWNLAFTAGQR
                     RFVFIRATRGGTTGVDQPQGTPGGGASSTLSRRYDDPRFVQNITRATAAGMLAGPYHF
                     ARPDVAGNTGADEADHFIQMAGAW"
BASE COUNT          530 a         1032 c          946 g          558 t
ORIGIN      
        1 gcttccgagt cggggctccc catcgagaat tccccgcccg gaatgggcac catgacatag
       61 ctgacctgcg tgcccgggat cgtgttggtg tacaacttca tgtcgttcgc gctccggatg
      121 gcctctttct cgaggatggt gacgattctc tcgtggatgt ccggcacggt cgcgtcgtcg
      181 ttgtcctgca tcgacgccag tttcaccagc tcgcccgcgg cgtaggctgc gccctcgggc
      241 ttgaaagccc gcgcgtccgt ggcgaacttc atgtagtcgg cgcccgtaaa caccagaaaa
      301 ctctcatcga gaccgaccgc cccgaaaatc aggtcgcccg atgtcgccgt gatcgattgc
      361 accgccccgg gggcgcccgc cgcgtcgagg gcaagcacct cggccgattc gcctttgccg
      421 taaacagcca gcaagcctgc gccctgggcc ttgggaaccg ccacaaggag catgatcggg
      481 ctggccagtt catatgtctt ggcctcgccg acgccgagtt tgccgcccgc ctcggtcaga
      541 ggatggaact ggatggattt ctcgcccttc ttgtacgtga ccacgtccgc caagggcgcg
      601 ccgcggaaga acccgacgac gtagtccgag cccttgggca gtccgcccgc ctccgccaca
      661 atcacctcgc tcccgctggc gaagtccgag acgcggaacg cgtcgctgtc ctcgccgctc
      721 caaagtcccg tgacatagag cggtccgccg cgtttgagcg ccaaccggtt cgggtgcacc
      781 cacgtcgcct tgatctcggg ctgcgcattc acggcgaagc tgccggcgtc attccggaac
      841 aggacaatct tgttgggatc gtcatagacc gaactgaccg cgagatcgtc gaggggcgtg
      901 ttgccagggc cgccgatgtc cacggccacc agcgcgccag ggccgagaga ctcgaactcg
      961 atcgccaccg gtttgcctgg ggaaccaggg tttgctgcct cgaccacatg gaccagattg
     1021 gcgtcggctg ccgtgaacgc gagcgcatcc ttgtcctccg acaccagcct gcccagagtc
     1081 agccaggtgg ggtccttgag gccgctgacc cggtagttta cccagttgaa gagcccgtct
     1141 gccgactggt acccgacccg gtacttgccg gtggcgcggt cgaaaatcac cacgtcctgc
     1201 cgacgattgc cgtccagatc gccggtgcca atcaactcgt ggtccgtctc gtacacgaag
     1261 ccccaggcga ttccagcccc cgccacgctc aaggccacca cccacgccgc agatcgtatc
     1321 gtatgcgtca ttcgtttcat cacgttcgca aaaaatgtcg cgtactcttt ccgcacggac
     1381 cggttttgtc aattgggcgg accaggattc cgcggggcgg agggacgagg caagaatccg
     1441 cgccgattcg tcccgtgttg cgagacatct gcgaagcggt gccgaagcgc acgcacttca
     1501 cagactgcgc tcctccgcgc gcgggcggcc gaggtcgaga ggcgcgccgg agcgccggct
     1561 cgcagccggc ttaacacgcg cgctgcgtgc attgttccac gcacccatcc cggaatctcg
     1621 tggcgtgagc gatgcgaaga ttccagccta ccgcagcttc accgccgttc cgctggccgt
     1681 caccatcaac atgccgttct gcgcgcccag gacctcgtaa tccagatcta ctcccagcac
     1741 cgcgtccgcg ccgcgcgcga cggccacttc ttccatctcg cgcaacgccg tggcccgcgc
     1801 atcggccagg acgcgctcat acgacgcgga gcggccgccg accagatctc gcacgctggc
     1861 gaacacgtcg cggaagaggt tggctccgat gatcgcctcc cccgtcgcaa tgccgagata
     1921 ctcctggacg agcctgccct cgatcgtgtg agttgtggtt gtgatcatac atgaccgata
     1981 cctcagaggc ggcgtgaagc aattgaaaag aagcgcaggc ccccccgaac aaggttgaca
     2041 ccaatatggt cagcgccaat tcatgaatac gaaaaggctg gaggggggac ggctcgtccc
     2101 ccgccatcgc gagtgcacac ggtgtttctc ggcgacgggg ccctgcctgc cctgcctgtc
     2161 cgcaggcagg cggcaggcag gcgtcgccgc tccacttcat gaattggctc tgtcccgcgg
     2221 atcgcgcgtc gctcagggcg gtgttcggtg tactgccttt aggcggctcg tggccaaaac
     2281 aagaaccgct gagaaaaacc ggcttctgct tgtgtctccc cctcatttcg gccactttca
     2341 cccgcttgtg aatcgaaccg caaaccattg atgtcgatgc tagcccgcat gtttcatgga
     2401 gtcgtgacca ccgcgaagga ggagactacg gattgtggac tactgcgggc tggaacggaa
     2461 gaccgcacct ggccagacga tcctgaggag ggcgacactc tgtcgggatg gagcggctca
     2521 ccgatcgatt ggcgcttgtc tgctggagtc gagtcaggcg cggttttgcc atctggtccg
     2581 aatccggttc agcgcgcagc ctgtgatcga tccgggccag gcgtcatcgt gccaaacgct
     2641 cgagcgggaa tcgccggagc gatccttgcc gcggccgtga tcgtatcggt gagcacggtt
     2701 tctcccgcgg cggctcaaga gcgggtgttg ggcgcggaca tttcctactg gaactgcgga
     2761 agctccagca ccgggatttc tcaggccaac tggaatctcg ccttcaccgc cggtcagcgg
     2821 cggttcgttt ttatccgggc cacgcgcggc ggcaccaccg gcgtagacca gccgcagggc
     2881 acccctggtg gcggcgcttc gtcgaccctc tcacggcggt acgacgatcc gcggtttgtg
     2941 cagaatatca cccgcgccac cgccgccggg atgctggccg ggccgtacca cttcgcccgc
     3001 ccggacgtgg cggggaacac cggtgctgac gaggcggacc atttcatcca gatggcgggg
     3061 gcgtgg
//
LOCUS       DAOQRO010000386         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36739_length_3062_cov_4.592950, whole genome shotgun sequence.
ACCESSION   DAOQRO010000386 DAOQRO010000000
VERSION     DAOQRO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36739_length_3062_cov_4.592950"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="PKK20_12125"
     CDS             complement(<1..2504)
                     /locus_tag="PKK20_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV00677.1"
                     /translation="MSELLRQIDVDANARLVLSPDCTVAQAADAWQRFLRLETHRIRL
                     AHRRGASGRACCEAQARVFDRVLTSLLEKTSAEAGIEGAQPSSALALAAVGGYGCRDL
                     SSASDIELLILRDDGASTSGGWRTVVERVQAPDMAGLFGRVRALAQTTAGAVEAANRD
                     LWTKTALLDARLVAGDLRLFAQFQGDFAARCVVGQAEAFIAAREADREARRAKFGRPV
                     CLQEPHIKSGCGGLMDFQSLLWLAFFKHGCRTLLELETRGLVDQSERHDLDRAYDFLL
                     RVRNEAHYQAGRAADVLTKSIQPAVARGLGYAERSYSRRIERFMRDYYRHARTIDLLT
                     RSIQERLGLVPSPPRPFALPARRAGAGGGSPAYVMDGFEFVEDRIRAAGPGVFREDPA
                     RLMRVFRHAQQRGARLDPGTVRLVREEATRAGAMLRSVPGAAQTFLEVLNQRGDVAPT
                     LRAMHESGVLGAYLPAFGRLTCLVQHEFFHQYTADEHTLACIAKLDELAGCRDGPRAA
                     YGDIFNKAIERPYLLYLALLLHDAGKARSTKDHAEVGGRIAAGVARRLGLDAASSATL
                     RFLVRHHLDMVTVSQRRDLDEPAEIRRFAALLGSVDRLNLLTLLTIADSLGTSDDLWN
                     GFKDSLLLTLHERTRTILEGERAFARAESRQRERLAEEVRTLLPEAFPADEIAAHFAR
                     MPPRYFLLQEARSIAADLELAHRFIERALAEDEDPLEPVTQWQHDRDRGYSVVRIATW
                     DRSGLFSRFCGALTAAGLSVLSAEVFTRDDSIVIDRFIVVEAHRGTPAGDEEQLRVAQ
                     YLREARQAGRDLREAIEACRKGQPARIEANWDPIPTV"
     gene            2755..>3062
                     /locus_tag="PKK20_12130"
     CDS             2755..>3062
                     /locus_tag="PKK20_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00678.1"
                     /translation="MDQSVPVADRLLGRYGPGSIAGISFDKGFTRAANRELLRLYIPD
                     VVMPKRGRKSATQTAAESTKTFVALRRRHSAVESDIAAREHHGLEQKLQNGHKRGSPS
                     "
BASE COUNT          566 a         1050 c          961 g          485 t
ORIGIN      
        1 acagtgggga tgggatccca attggcctcg atgcgggccg gctgcccttt ccggcaggcc
       61 tcgatcgcct cacgtaaatc ccggccggct tgtctggcct ccctgagata ctgggcaacg
      121 cgcagttgct cctcgtcgcc cgcgggcgtc ccgcggtggg cctcgaccac aatgaacctg
      181 tcgatgacga tcgagtcgtc gcgagtgaac acctcggcgc tcagcacgct caggccggcg
      241 gctgtcagcg ccccgcagaa ccggctgaac aagccgctcc gatcccaggt ggctatccgc
      301 accaccgaat aaccgcggtc acggtcgtgc tgccactggg tgacaggttc gagcggatct
      361 tcgtcctcgg ccagggcgcg ttcgataaac cggtgcgcga gctcgagatc ggctgcgatg
      421 gaccgggcct cctgcaacag aaaataacgc ggaggcatcc gtgcgaaatg agccgcgatc
      481 tcgtccgcgg ggaacgcctc cggcagaagc gtccgcactt cctcggccag gcgctcccgc
      541 tgccggcttt ccgcccgggc gaacgcgcgc tcgccttcga ggatcgtgcg ggtccgctca
      601 tggagggtga gaagcagcga gtctttgaac ccgttccaga gatcgtcgct cgtcccgagc
      661 gagtcggcaa ttgtcagcag agtcagcaga ttcaaccggt cgacgctccc aagcagcgcc
      721 gcgaatcgcc gaatctccgc cggctcgtcc agatcgcgac gctgcgacac agtgaccatg
      781 tcgagatgat gccgcaccag gaaacggagt gtggccgacg atgcagcgtc gaggcccagc
      841 cgcctcgcca cacccgccgc aatccgtccg ccaacctcgg catggtcctt ggtcgagcgc
      901 gccttgcccg catcgtggag caacagagcg agatagagga gatagggccg ttcgatcgcc
      961 ttgttgaaga tgtcgccgta agcggctcgg gggccgtccc tgcatccggc cagctcatcc
     1021 agtttggcga tgcaggccag ggtgtgttca tccgccgtgt actgatgaaa gaactcgtgc
     1081 tggaccaggc acgtcagcct gccgaatgcc ggcaggtacg ctcccagtac acccgactcg
     1141 tgcattgccc ggagcgtggg cgccacgtcc ccccgctggt tgagcacctc gagaaacgtc
     1201 tgcgccgcgc cggggacgga acgtagcatg gcccccgcgc gcgtcgcctc ctcgcgcaca
     1261 agccgcaccg tgcccggatc gagcctcgcg ccgcgctgct gcgcatgccg aaaaacacgc
     1321 atcagccgcg caggatcctc gcgaaacacg ccgggtccag ccgcccgaat gcgatcctcg
     1381 acaaactcga atccgtccat gacgtacgcg ggcgatccgc cccccgcccc cgccctccgg
     1441 gcgggcagcg cgaaaggccg cggcggcgaa ggcacgagtc caagccgttc ttggatgctg
     1501 cgggtcaaca gatcgatcgt tcgggcgtgt cgataatagt cgcgcatgaa acgctcgatc
     1561 cgccggctgt aggaacgttc cgcatagcca aggccgcgcg ccacagccgg ttggatgctc
     1621 ttggtgagaa catccgctgc ccgccccgcc tggtagtgcg cctcgttcct cacccgcaga
     1681 agaaaatcgt aggcacgatc gaggtcatgc ctctcggact gatcgaccag gccccgcgtc
     1741 tccagctcga ggagggtgcg gcatccgtgc ttaaaaaacg ccagccagag cagactctga
     1801 aaatccatga gcccgccgca tccgctcttg atgtgcggtt cctgcagaca gacggggcgc
     1861 ccgaacttgg cccggcgcgc ctcgcggtct gcctctcgcg cggcgatgaa cgcctccgcc
     1921 tggcccacga cgcaccgggc cgcaaagtcc ccctggaatt gggcgaacag ccggaggtcc
     1981 ccggcgacca accgcgcatc gagcaatgcg gtctttgtcc acaaatcccg attcgctgcc
     2041 tcaacagctc cggctgttgt ctgcgccagt gcccgcaccc gcccgaacag accggccata
     2101 tcgggcgcct ggaccctctc gaccaccgtg cgccatcctc ccgatgtcga tgcgccatcg
     2161 tcgcgcagaa tgagaagctc gatgtcgctg gccgaactca gatcgcggca cccatagccg
     2221 cccacggctg ccagcgcgag ggctgagctc ggctgcgccc cctcgatgcc ggcttccgcg
     2281 gacgtcttct ccagcagact cgtcaagacg cggtcgaaga cccgcgcttg cgcctcgcaa
     2341 cacgcgcgtc cggacgctcc gcgccgatgc gccaatcgaa tccggtgagt ttcgagtcgg
     2401 aggaatcgct gccacgcatc cgccgcttgg gccaccgtgc agtccggaga caagaccagc
     2461 cgggcgttgg cgtcgacatc gatttgtcga agcagttccg acatccgggc ggaaagtagg
     2521 ccaccgccat gccggagcgc aagccgatgc gcaagcacca cgtaccgcag cggctgactc
     2581 tctttgtgtc cgaactcata atcccccgcc ggccgcgtct accgcgtccg tcgatcaacg
     2641 tcagcaggtc acccgccagg ccgaacgaga actggctgac ttacagattc cagttggtct
     2701 cggcgacctt gcgggttggt ccaggactac gatgaattaa tcggccgagt agacgtggat
     2761 cagagcgtgc cggtggccga ccggttgctg gggcgttacg ggccggggtc gatcgccggc
     2821 atcagcttcg acaaagggtt cacgcgggcg gccaaccggg aattactgcg gctctacatt
     2881 ccggacgtgg tgatgccgaa gcgaggacgc aagagcgcga cgcaaacggc agcggagagc
     2941 accaagacat ttgtggcgtt gcgacggcgg cacagtgccg tggagagtga cattgcggcg
     3001 cgggagcatc acggactgga gcagaagctg caaaatggcc ataaaagagg ttcgccgagc
     3061 aa
//
LOCUS       DAOQRO010000387         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_36893_length_3054_cov_4.982994, whole genome shotgun sequence.
ACCESSION   DAOQRO010000387 DAOQRO010000000
VERSION     DAOQRO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36893_length_3054_cov_4.982994"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..275
                     /locus_tag="PKK20_12135"
     CDS             123..275
                     /locus_tag="PKK20_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNV00679.1"
                     /translation="MWALLKEFLVFLRQEKKWWLVPLVVLLLGVGALLIFGTSSGIAW
                     ALYPFM"
     gene            292..2127
                     /locus_tag="PKK20_12140"
     CDS             292..2127
                     /locus_tag="PKK20_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNV00680.1"
                     /translation="MAGPVHILGLSAYYHDSAACLLRDGAIVAAAQEERFSRVKNDAQ
                     FPRHALEFCLRHGAIKPAQLDAIVFYDKPILKFTRMLESYLAVAPAGWRTFAGILPSW
                     FSEKLDLRKTIRDQVPDLSASVPILFTEHHQAHAAAAFFPSPFRESAIVTCDGVGEWA
                     TTTIGLGRRTELELLKELRFPHSLGLLYSAFTSYCGFRINSGEYKLMGLAPYGEPMYE
                     NTIRRDLIDLKEDGSVWLNLDYFDFLRGTTMTNERFHRLFGGPPRPPEGPIERRHLDV
                     ARSIQVVTEEAMLRLARHAREVTGLPHLCLAGGVALNCVANGRILREGLFERLWIQPA
                     AGDAGGALGAALAAWHSRWQGPRGRPDDDNPEADAMQSALLGPSFTDGEIEDALRIHG
                     AVYERLDSDALLRRVVAWLAAEKVVGWFQGRMEFGPRALGNRSILGDPRSPRMQSMMN
                     LKVKFRESFRPFAPVVLRENAADYFELETASPYMLLVAPIRPALRRPVPAGLEGLARL
                     AAVRSDLPAVTHVDYSARIQTVSQRQNPRLYDLLQCWRAATGCAVLVNTSFNVRGEPV
                     VCSPDDAYRCFVNTEMDALAIGSFVLERGAQPSRQIARRVPLQPD"
     gene            2142..2543
                     /locus_tag="PKK20_12145"
     CDS             2142..2543
                     /locus_tag="PKK20_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00681.1"
                     /translation="MKLVLQEDPRQWRRFALTALPAPALLGLLLWWKRVVPAPGLLLW
                     LGVLLGLALLALWRPTWFRGFYRAGMTAGFHASRVLGWIALSLLFFLALTPLGLVLRW
                     SGHDPLQLRRQRQAASYWRRPRQKHDLDRMF"
     gene            complement(2569..>3054)
                     /locus_tag="PKK20_12150"
     CDS             complement(2569..>3054)
                     /locus_tag="PKK20_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNV00682.1"
                     /translation="SRPSAVPPVQTSGSTALAQGGMAGIGFWIAGVPGAALLALLTCF
                     LSFVPVGPPMVWIPAVIWLFNQGSIGWAIFMLIWGLGVSSVDNVIKPWLISQGSAMPF
                     ILIFFGVLGGALGFGVIGVFLGPTLFALLYRLVEEWSAVRPGGIPHVPEPPIEEAEAN
                     A"
BASE COUNT          519 a         1023 c          927 g          585 t
ORIGIN      
        1 tatttccaac ctcaacaaca gcaacccttc aggaatttaa atgaaataac cgtgccggaa
       61 tgacgcggtg cccgaatgat gcagttgccg ggcggcaatc cgtcggttaa tctccctttc
      121 cgatgtgggc gctcttgaag gaattcctgg tcttcctgcg ccaggagaag aagtggtggc
      181 tggtgccgct ggtggtgctc ctgcttggtg tgggggcgct tctgatcttc ggcaccagtt
      241 cgggcatcgc ctgggcgctt taccccttca tgtgagccag cctcgtcaaa catggccggc
      301 cccgtccata tcctaggtct ttcggcatac tatcacgact cggcggcctg cttgctgcgc
      361 gatggcgcga tcgtcgccgc ggcgcaggaa gagcgcttct cgcgtgttaa gaacgacgcg
      421 caattcccca ggcacgcgct cgagttttgc ctcaggcacg gggcgatcaa gccggctcaa
      481 ctcgatgcca ttgtctttta cgacaagccg atcctgaagt tcacgcggat gctcgagagt
      541 tacctggccg tcgccccggc cggctggcgc acgttcgctg ggattctgcc cagttggttt
      601 tccgagaaac tcgacctgcg caagacgatt cgggaccagg tcccggactt gtcggcatcg
      661 gtgcccattc ttttcacaga gcaccaccag gcccatgccg cggccgcctt ctttcccagc
      721 ccgttccggg aatcggcgat tgtcacgtgc gacggtgtgg gtgaatgggc caccacgacg
      781 atcggtttgg ggcgccgaac ggaactcgag ttgctcaagg agctgcgctt cccccattcg
      841 ttgggtctgc tctattccgc cttcacgtcc tattgcggat tccgcatcaa ctcgggcgag
      901 tacaaactga tggggttggc accttatggc gagccgatgt acgagaacac gatccggcgc
      961 gacttgatcg atctcaagga ggacggctcc gtctggctga atctggacta cttcgatttc
     1021 ctgcgcggca cgaccatgac gaatgagcgc tttcaccggc tcttcggcgg cccgcctcgt
     1081 ccgcccgagg ggcccatcga gcgccggcat ctcgatgtcg cgcgctcgat tcaagtcgtg
     1141 accgaagagg ccatgctgcg gctggcccgg catgcgcgcg aggtgaccgg tctgccgcac
     1201 ctctgcctgg cggggggcgt ggcgctcaac tgcgtcgcca acggtcgcat cctgcgcgag
     1261 ggtctgttcg aacgcctctg gattcagccg gcagccggcg atgcaggagg cgccttgggg
     1321 gcggcactgg cggcctggca cagccgatgg cagggtccaa ggggccggcc cgacgacgac
     1381 aatccggaag cggatgccat gcaatcggcc ctgctcggcc cttcgttcac cgacggcgaa
     1441 atcgaagatg cgctgcgcat ccacggggcg gtgtacgagc gcctggattc cgatgccctg
     1501 ctccgccgag tcgtcgcatg gctggccgcc gagaaggtcg tgggctggtt ccaggggcgc
     1561 atggagttcg ggccgcgcgc cctgggcaat cgctcgatat tgggcgatcc gcgatcgccc
     1621 cggatgcaat cgatgatgaa tctcaaggtc aagttccgcg agtcgtttcg ccccttcgcc
     1681 cctgtggtgt tgcgcgaaaa cgccgccgat tatttcgaac tcgagacggc gtccccatat
     1741 atgctgctcg tggcgccgat ccgacccgcc ttgcgccgcc ccgttccagc cggcctcgag
     1801 gggctggccc ggttggcggc ggtccgatcg gacctgccgg ccgtcacgca cgtcgactac
     1861 tcggcccgca tccaaaccgt cagccagagg caaaaccccc gactctacga tctcctgcag
     1921 tgctggcgcg ccgcaacggg ctgcgccgtt cttgtcaaca cgtcgttcaa cgttcgaggc
     1981 gaaccggtcg tctgctcccc cgacgacgcc tatcgctgtt tcgtaaacac cgagatggat
     2041 gcgttggcga tcggcagttt tgtgctcgaa cgcggtgccc agccctcccg ccagatcgca
     2101 cgacgcgtcc cgctgcaacc ggattgaccc tcgacacccg tgtgaaactc gttctccagg
     2161 aagatccccg ccagtggcga cgtttcgctc tgactgcctt gcccgccccg gctctcctcg
     2221 ggctgctcct gtggtggaag cgcgttgtgc ccgctcccgg cctcctgctc tggctgggcg
     2281 tcctgctcgg tctggctctc cttgccctgt ggcgcccaac ctggtttcgc ggattctatc
     2341 gcgccgggat gactgccggc tttcacgcaa gccgtgtcct gggctggatc gccctgagcc
     2401 tcctcttctt cctcgccctc accccgttgg gtctggtgct ccgatggagc ggacacgatc
     2461 cgctgcaact gcggcgccag agacaagccg caagctattg gcgccgcccc cggcagaagc
     2521 acgacctcga ccgcatgttc tgatcggtca aggaacacgt gttcacgctc atgcgttcgc
     2581 ttccgcctct tcgatgggcg gctcaggcac gtgggggatg ccgcccggac ggacagccga
     2641 ccactcctcg acaagtcggt agaggagcgc aaacagcgtc ggacccaaaa acacgccgat
     2701 gactccaaag cccagcgcgc cgcccagaac gccaaagaag atcaggatga agggcatggc
     2761 gctgccctgg ctgatcagcc acggtttgat gacgttgtcc acactgctga cacccagacc
     2821 ccagatcagc atgaagatgg cccaaccgat cgaaccctga ttgaagagcc agatcaccgc
     2881 cggaatccag accatcggcg gtcccaccgg tacaaacgag aggaagcacg tcagcaacgc
     2941 aagcagggcc gcgcccggaa cgccggcaat ccagaaaccg atccccgcca tcccgccctg
     3001 agcaagcgcc gtgcttccac tcgtttgtac cggtgggacc gccgatggtc tgga
//
LOCUS       DAOQRO010000388         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_37297_length_3034_cov_3.835515, whole genome shotgun sequence.
ACCESSION   DAOQRO010000388 DAOQRO010000000
VERSION     DAOQRO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37297_length_3034_cov_3.835515"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKK20_12155"
     CDS             <1..865
                     /locus_tag="PKK20_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00683.1"
                     /translation="RLVLQIGKRAAEKNLKVAGGLMSRHSKPLEEAVRQIHDGLIGEV
                     ITAWAYRMHGPVSVSPRQAGMSELAYQIANYSCFTWLNGSFIVDWLIHNIDVCCWVKN
                     AWPVSAQGMGGRQVRTDADQLFDHYAAEYTFADGTRLFAQGRHMTRCYDFFGDIIHGA
                     KGCGILGEGQPKPRLFKGHNLASENLAWQYQGPPCDQYQVEHDLLFDAIRTDKPYNET
                     EHCAKSCFTAIMGRMACESGQMITWDDAFASNLELAPGLEQLTMDADAPVLPDANGRY
                     PIAIPGQTKVI"
     gene            complement(1070..2614)
                     /locus_tag="PKK20_12160"
     CDS             complement(1070..2614)
                     /locus_tag="PKK20_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00684.1"
                     /translation="MKTMPLLLALIAFPLCAALTQSAPVKVFTIFTSGGCGCTGSGGA
                     PASFMTHDEVVRNLQAACTGVDFVRWEGAAPAGYDEVKNHPGKYDGILIVGRMSGDYR
                     LAFTGLPTIVVYNLFEFMDAHPYHLFATGQTDEQNNVLKNGTNYSNGRILTAQLDRRN
                     LSAPSVREAMFQDLVYKIRLIQVVVELKKTRILMVKNQTNEIIASVNYRGDVNQTFPP
                     DHNERYARNLKELFGVEIVPVEADEFFEAYKNVDLKQAEEVAEEWIRGARKVEAGKSE
                     IIKTARGYLALDFLRTKHQCNAVSTHIRTVTGSGKREDLFNPGLGLELGFKRRGIQAV
                     CQNYPDLLVGQVLGHLLTGRPSMLGDFFYDTYNFVEVILHCGIPVNPYGDDRIMPYTI
                     RPHAESPVRDKPDEPGSSTGITAEWPVGEPVTLWEVHALNREIRLHTGQIVNGKEVYG
                     GSEDLDNVMCTAKVVACIENAEKLQREFRPYLYGIHRNATLGDLRQQIKDVGVFLGIP
                     VVERDR"
     gene            2838..>3034
                     /locus_tag="PKK20_12165"
     CDS             2838..>3034
                     /locus_tag="PKK20_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00685.1"
                     /translation="MKTCENRTSHTPRTGFAALAAATVLLTPLALGQLQIDSLGANGL
                     LSWSDPGGTGTHYAVQWSSSGL"
BASE COUNT          592 a          946 c          893 g          603 t
ORIGIN      
        1 ccggctcgtc ttgcagatcg gcaagcgcgc cgccgagaag aacctcaagg tcgccggcgg
       61 cctcatgagc cgacactcga aaccgctcga agaagccgtg cggcagatcc acgacggcct
      121 catcggcgag gtgatcaccg cctgggccta ccgcatgcat ggtccggtca gcgtcagccc
      181 acgccaggcc ggcatgagcg aattggccta ccagatcgcc aactacagtt gcttcacctg
      241 gctcaacggc agcttcatcg tcgattggct catccacaac atcgatgtct gttgctgggt
      301 caaaaacgcc tggcccgtct ccgcccaggg catgggcgga cgccaggtcc gcaccgacgc
      361 cgaccagttg ttcgaccact atgccgccga gtacaccttc gccgacggca cccgcctctt
      421 cgcgcagggt cgtcatatga cccgttgcta cgacttcttc ggcgacatca tccacggggc
      481 caaaggctgc ggcatcctcg gcgaaggcca gcccaaaccg cgcctcttca aaggccataa
      541 cctcgcctcc gaaaacctcg cctggcagta ccagggcccc ccgtgcgatc agtaccaggt
      601 cgaacacgac ctcctgttcg acgccatccg caccgacaag ccctacaacg aaaccgagca
      661 ctgcgccaag tcgtgcttca ccgccatcat gggacgcatg gcctgcgaat cagggcagat
      721 gatcacctgg gacgacgcct tcgcctcgaa tcttgaactg gctcccggcc tggaacaact
      781 gaccatggac gccgatgccc cggtcctgcc cgacgccaac ggacgctacc ccatcgccat
      841 ccccggacag accaaggtca tctagcttgt gtcgcataat gtccggaact tcggcggcgc
      901 gggtcgcttc ggcatcgaag cgatcacccc agccgagatg ctcagggaac agcgaggaat
      961 gacatgacga cgatcgaaga ggtccttggc acagaccctt agcaaccagg cggacaagcc
     1021 accgactcga tcctcgaccc ggcctctgtg acggttctga tgttctgtgt taccggtccc
     1081 gttccacgac cggaatgccc aggaacacgc cgacgtcctt gatctgctgg cgcaggtcac
     1141 ccagggttgc gttgcggtga atcccgtaga gataaggccg aaactcgcgc tggagtttct
     1201 ccgcgttctc gatgcacgcg acgactttgg ccgtgcacat gacgttgtcc aaatcctcgg
     1261 acccgccgta gacctccttg ccgttcacga tttggccggt gtgcaaccgg atctcccggt
     1321 tgagcgcatg cacctcccag agggtcacag gctcgccgac gggccattcg gccgtgattc
     1381 ctgtcgagga gccgggctcg tccggcttgt cccggaccgg gctctcggca tgcgggcgaa
     1441 tggtgtaggg catgatgcgg tcgtcgccat aaggattgac cgggattccg caatgcagga
     1501 tgacctcgac gaaattgtag gtgtcgtaga agaaatcgcc cagcatgctg gggcggccgg
     1561 tgagcagatg accgagcacc tggcctacca ggaggtcggg atagttctgg cagacggcct
     1621 gaattcccct tcgtttgaag cccagttcga gcccgaggcc gggattgaac aagtcctcgc
     1681 gtttcccgct gccggtgaca gtcctgatgt gggtggacac ggcattgcac tggtgcttgg
     1741 tcctcagaaa gtcgagggcg aggtatcccc gcgcggtctt gataatctcc gatttgccgg
     1801 cttcgacctt gcgggcgccc ctgatccatt cctcggcaac ctcttcagcc tgcttgaggt
     1861 cgacgttctt gtaggcttca aaaaactcat cggcttcgac tggcacgatt tcaacgccga
     1921 aaagctcctt gaggttgcgg gcgtaacgtt cgttgtgatc gggcgggaag gtctggttga
     1981 catctccgcg gtaattgacg ctggcgatga tctcgttcgt ctggttcttc accatcagga
     2041 ttcttgtctt cttcaactcg accaccacct gaatgagcct gatcttgtag accaggtcct
     2101 ggaacatcgc ttcccggacc gatggcgccg agaggttcct gcggtccagt tgggccgtca
     2161 ggattcgccc attcgagtag tttgttccgt tcttgaggac gttgttctgt tcatcggttt
     2221 ggccggtggc aaacaagtga taaggatggg cgtccatgaa ctcgaacaga ttgtagacca
     2281 caatggtcgg caatcccgtg aaggccaggc ggtagtctcc agacatcctg ccaacgatca
     2341 ggatgccatc gtatttcccg gggtgattct tcacttcgtc ataaccggcc ggggccgcgc
     2401 cctcccaccg gacgaagtcg acgccagtgc atgcggcctg gaggttcctg acaacctcgt
     2461 catgcgtcat gaagctcgct ggggcgcctc ccgagccggt gcagccgcac ccgcccgagg
     2521 tgaagatcgt gaagactttg acaggagcgc tttgggtaag cgcggcgcac agagggaacg
     2581 cgatcaatgc gagcaggagc ggcatggttt tcatgaggtg tgagaggaag tgtttggcgg
     2641 ttggcagggc attcgttgcg gaggcgatct taggcgaacg ccagccttgt gcaaatcaat
     2701 cccgcggcag ccctgcgcga attcgtcgtg gattctgaaa aaccttggcg cgtgtgtcct
     2761 gaaggaaaac ctcttgcggt tggcggccat catcatagag tcatcaccaa atggaacgac
     2821 gcagtctgct tgatcgaatg aaaacctgcg aaaacagaac ctcccacaca cctcgcacgg
     2881 gattcgcggc gctcgccgcc gcaacggtcc ttctcacacc cctggcgctc gggcagttgc
     2941 agattgattc cttgggtgca aacggactct tgagctggag cgatccgggc gggaccggga
     3001 cgcactacgc ggtgcagtgg tcttcttccg ggct
//
LOCUS       DAOQRO010000389         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_37369_length_3030_cov_4.875630, whole genome shotgun sequence.
ACCESSION   DAOQRO010000389 DAOQRO010000000
VERSION     DAOQRO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37369_length_3030_cov_4.875630"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PKK20_12170"
     CDS             <1..885
                     /locus_tag="PKK20_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNV00686.1"
                     /translation="HTVFALPFALAAMAVAARDQCGWPGWRTFALILAAMVCARTCAM
                     AFNCIADRRFDAANPRTAGRHLPAGTVSLAGALAFWAMSAIGFIGAAYLINRPCFYLS
                     PVALVVICFYSFTKRFTDFTHIFLGIALALAPLGAWLAVTGTFEFLPVRDGALSMRHS
                     ALLAVLLAIAVVFWLIGFDIIYALQDYAFDRSHGLHSLVVRWGVKNALGASFLSHFIM
                     WMVLAVFGFLARFWVAYMIGLGIIAFCLVLEHWLARKRSLKWINVAFFRLNALISVVF
                     LVVTVAEVAFPRFRYLAR"
     gene            complement(897..1598)
                     /locus_tag="PKK20_12175"
     CDS             complement(897..1598)
                     /locus_tag="PKK20_12175"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNV00687.1"
                     /translation="MKICAFGLADLPLGKHNLKDPRLDKAHELVEAKKKAYAQVDLVG
                     EDESLEADAILVHRDRFPDLILHDLEFVETRLGRNPPDAERVVLEKIRNELESERTVY
                     KAGLGKDELEAVAAHSFHTHKPVIAADASDLEQPDALMLRAFAEGGWISFLTVGGKEN
                     RAWPVMSGTTAWEAAGTIHSDMQKGFIRAEIINYADFLEAGGETEAKRAGKLRLEMKS
                     YVIQDFDLANFRFSK"
     gene            1876..2376
                     /locus_tag="PKK20_12180"
     CDS             1876..2376
                     /locus_tag="PKK20_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNV00688.1"
                     /translation="MTVEQLIGFVLALVLMGVGLVGCVVPGLPGTPLVLGAAVLHRIY
                     FGASSVGNAALALLVGLALLALLLDYLASVLGARALGATWRGMIGAIVGLACGLLGGP
                     VGIVVGPFLGATGLEMLSGRDIRQASRAGLGAFLGLLVGGMGKIACCVAMIGLFTFNV
                     IAGAMP"
     gene            complement(2497..2796)
                     /locus_tag="PKK20_12185"
     CDS             complement(2497..2796)
                     /locus_tag="PKK20_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00689.1"
                     /translation="MAGEFLKSDPSYRVETGRQVERTAIEIAACARPLLRVSEELVLV
                     EPNFKVVDDYNRLVYRFLDTLEELPRQRQKMPHPLKRLELHLERSPREVCSRHSA"
BASE COUNT          442 a          938 c          984 g          666 t
ORIGIN      
        1 cacacggtgt ttgcgctccc tttcgccctg gctgccatgg cggtcgcggc gcgggaccag
       61 tgcggctggc ccggctggcg cacgttcgcg ctgatcctgg cggcgatggt ctgcgcccgc
      121 acctgcgcca tggctttcaa ctgcattgcg gaccggcgtt tcgacgccgc caatccgcgc
      181 acagcggggc ggcacttgcc tgccggcacg gtgagtctgg cgggggccct ggccttttgg
      241 gcgatgagcg cgatcggctt catcggcgcc gcctacctga tcaatcgccc gtgcttttat
      301 ctctcaccgg tggctctggt ggtgatttgt ttctattcgt tcacgaagcg gtttacagac
      361 ttcacccaca tttttctcgg cattgcgctg gccctcgcgc cgctcggggc ctggcttgcg
      421 gtgaccggca cgttcgagtt cctgcccgtg cgcgatggag cgctgtcgat gcgccacagc
      481 gcgctgttgg cggtgttgct cgccattgcg gtggtgtttt ggctgatcgg gttcgacatc
      541 atctacgccc tccaggacta cgcgtttgac cgatctcacg gcctgcactc gctggtggtg
      601 cggtgggggg tgaagaacgc cctcggcgcc tcctttctct cgcatttcat catgtggatg
      661 gtgctggccg tatttggttt tctggcccgc ttctgggtgg cctacatgat cggcctgggc
      721 atcatcgcct tttgcctggt tctggaacac tggctggcgc gcaagcgcag cctcaagtgg
      781 atcaatgtcg ccttcttccg cctgaacgcg ttgatcagcg tcgtgttcct cgtcgtcacc
      841 gtggccgagg tcgcctttcc gcgtttccgg tatctggcgc ggtgatccgg cgggctctac
      901 ttgctgaacc ggaagttggc cagatcgaag tcctggatga cataggactt catctcgaga
      961 cggagcttgc cggcccgctt ggcctcggtc tcgccccccg cctcgaggaa gtcggcgtag
     1021 ttgatgatct cggcccgaat gaaacccttc tgcatatcgg agtggattgt gccggcggct
     1081 tcccaggcgg tggtccccga catcaccggc caggcgcggt tctccttgcc tccgacagtg
     1141 aggaaggaaa tccagccgcc ttcggcaaac gcccggagca tgagtgcgtc cggttgttcg
     1201 aggtccgatg catcggcggc gatgaccggt ttgtgcgtgt ggaagctgtg ggcggcgacg
     1261 gcttccagtt cgtccttgcc aagtcccgcc ttgtacaccg tccgctcgct ctcgagttcg
     1321 ttccgaattt tctccaacac gacccgctcg gcgtcgggcg ggttgcgtcc gagacgcgtt
     1381 tcgacgaatt cgaggtcgtg gaggatgaga tcggggaacc tatcgcggtg gaccaggatg
     1441 gcatcggcct cgagagattc atcttctccg accaagtcga cctgggcata cgccttcttc
     1501 ttggcctcga ccaactcgtg ggccttgtcc agacgcggat ccttgaggtt gtgtttgccc
     1561 agcggcaaat ccgccagacc gaacgcgcaa atcttcatcg gccatgatta gcgtctgtct
     1621 cctgcctgtt tcaacaggaa tccgcccgga tgttgctgca gagtcggtcg gatacgctgc
     1681 gcacttccct cactccggcc ctctcccatc cgacctgcct gccgaagccc gggcgcaggc
     1741 aggtgggaga gggtgtcccg cgatgcggga cgggtgagga taactgtata gtgcatcctg
     1801 gtgattcctc ggtgaatcca aaaggaggcg ctctccccgt tgcggcgcgc accgaatccg
     1861 ctacatttct gcccgatgac ggtcgagcaa ctgattggat tcgtcttggc gctggtattg
     1921 atgggagtcg ggctggtcgg gtgcgtcgtg cccgggctgc cgggaactcc gctggtcttg
     1981 ggggcggcgg ttctacatcg aatctacttt ggcgcatcga gtgtggggaa cgcggcgctg
     2041 gctttgctgg tcggtctcgc cctgcttgcg ctgctgctcg attacctggc cagcgtactc
     2101 ggcgcgcgcg cgcttggggc cacctggcgc gggatgattg gtgccatcgt gggtttggcg
     2161 tgcgggctcc tgggtggacc ggtgggcatt gtggtcggac cgttcctggg agcaacaggc
     2221 ctcgagatgc tgtccggacg cgatatcagg caggcgagcc gggccggctt gggcgccttt
     2281 ctgggcctgc tggtgggcgg catgggcaag atcgcctgct gcgtcgcaat gatagggctc
     2341 ttcaccttca acgtgatcgc gggcgcaatg ccctgacggg gagagccagt cagcaggtcg
     2401 gcggagtgct cgacgctggg ttcgagaggg tcagtccgcc gatgcgaatg cgatcgatgc
     2461 ccgttcggca cccgcatcgc cggtctgagc tcaaaatcat gctgaatgcc ggctgcagac
     2521 ctccctgggg ctccgctcca agtgcaattc gagccgtttg agcggatgcg gcatcttctg
     2581 ccgttgccgc gggagttctt cgagcgtgtc caggaaacgg tacacgagcc gattgtaatc
     2641 atccacgacc ttgaagttgg gctccaccag caccagttcc tcgctgaccc gcaaaagcgg
     2701 tctggcacaa gcggcaatct cgatcgccgt ccgctccacc tggcgccccg tctcgacacg
     2761 gtacgaaggg tcggatttca agaactcgcc agccacgagc acccggagat gctgttgtgg
     2821 gttgccctcc gcgacgattc catggaaagc ccgatccccg ttaagacccg tcgcgttcgc
     2881 gagccggggc ctggtaccgg ttcgccaggc ggggcgactc cgggtcctca agccgcgccc
     2941 ggtagcgcat atcggacacg aaggacttta ggttgccagc cggcgctgac agatgctgtg
     3001 cgcaataccc gcacgccgga cattcctgaa
//
LOCUS       DAOQRO010000390         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_37742_length_3010_cov_5.613536, whole genome shotgun sequence.
ACCESSION   DAOQRO010000390 DAOQRO010000000
VERSION     DAOQRO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37742_length_3010_cov_5.613536"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKK20_12190"
     CDS             <1..515
                     /locus_tag="PKK20_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNV00690.1"
                     /translation="DLKRDVVYRNDELYNLMQSLAIEGSIEGLRYEKVILATDADVDG
                     LHIRNLLITYFYRFFDRLVHDGHLHVLETPLFRVRNKDRTIYCYSEAEREAAIQSLGG
                     KPEITRFKGLGEINPAEFKQFIGKEMRLSRVEYAPRTEAGGILTFYMGKNTPERKEYI
                     MERLVVPVEE"
     gene            1204..2538
                     /locus_tag="PKK20_12195"
     CDS             1204..2538
                     /locus_tag="PKK20_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00691.1"
                     /translation="MTHRSKRISAAPSNLTLIRMTALAAWALLVVHTTWAADADTPPS
                     ASPAAGLPGVQADGTADDTAALQRVLDQAGQAGGQVLLPGARYKIAGSLRIPPGVTLQ
                     GVSESPVWSEPLKGSIILATGGRDQEDGPALFELGHSSAVRGLTVFYPDQHPTNIHPY
                     AWTFHLQGHDNTVENVTLINSYNGIRIGPEGNVRHRIRSVYGCVLRRGIFVDACTDIG
                     RIDNVQFHCHWWSAAAVGGDWKPVHEFMWKNCEAFIFGRTDWEYVNNTFVYPVNIGYR
                     FIRTQAGAANGQLSGIGADEAQVCVRVEHIQPMGLLISNGQFVCMHGDTRVPVLVEST
                     CQGSVRLVDCAFWGPNRQCVVSHSRSFVSLSDCYLSSTGRAKNPGVALIEADGGKLQV
                     RGCSFGTDEPGIALKKGLEHAIVTENNGVRGVEIRNEIGDKAIIANNEPELK"
     gene            2765..>3010
                     /locus_tag="PKK20_12200"
     CDS             2765..>3010
                     /locus_tag="PKK20_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00692.1"
                     /translation="MANPEAADRYPATLPLARLGDRPLARRSRRDATRPLRCDRDPDR
                     GERYDVLLTNPPFGTKGANQAPDRDDFTIETNNKQLNF"
BASE COUNT          604 a          958 c          919 g          529 t
ORIGIN      
        1 gggacctcaa gcgcgatgtc gtctacagga acgacgagct ctacaacctc atgcagagcc
       61 tggccatcga gggcagcatc gaggggctgc gctacgagaa ggtgatcctg gcgaccgacg
      121 cggacgtcga cggtctgcac attcgcaacc tgctcatcac ctatttctac cggttcttcg
      181 accgcctcgt gcacgacggc cacctccatg tgctcgagac accgctgttc cgcgtccgaa
      241 acaaggaccg cacgatctac tgctacagcg aggcggaacg cgaagcggcc atccagtccc
      301 tcggcggcaa gcccgagatc acccggttca agggtctggg cgagatcaac cccgcggagt
      361 tcaagcaatt catcggcaag gaaatgcgcc tcagccgcgt cgagtacgcg ccacgcaccg
      421 aggcgggcgg catcttgact ttctatatgg gcaagaacac ccccgaacgg aaggagtaca
      481 ttatggagcg ccttgtcgtg ccggtcgagg agtagttggc gaggctccgc ctcaaaccag
      541 cggggcattt caacagttcg tttgcgctcg gctttggcac tgacaatgaa actgagcgtt
      601 tcgtcgcgca gggtggtgtc gagaactttg atgccgccgg cgctgatcag gtccttcccg
      661 ttagggtctg tgcaaaaatt acttccgatt ttgctgaagg ggatttggct cgctggcgag
      721 gcgcgacgaa ggagcatagc cttgacctct gtgagtgagg agcaacgaag tcagcgagcc
      781 aaagcccctc cagcaaacat cgccacacgc gccggatcac ggcccctctg actggcgagt
      841 gaggtgcggc ggctctcgtg atgtcgtgtc ctctgcgcgc tctgcgtctc tgcggttcat
      901 tccgggaggg tgactgtcat cggatggaat tcaccacaga gacacagaga tcacagaggg
      961 aacagcacct catgaattcg gggcggatca tgaagcacgg atgacttccc gcgtttcctt
     1021 tcttgtgaga agaaccggag agaacgaagg tcgtgcaaag cacgaaggca gtgccgacag
     1081 gcgtttccgt gcccgtcgca acgtccggac tggtccgtcc gtctcagacc aatctcgatc
     1141 cccagctcgt cgggcctgga aatgccgtgc cttccgattg caccgtggta ggttttgggc
     1201 agtatgacac acagatccaa gcgcatctct gcagccccct cgaatctcac actcatccgc
     1261 atgaccgcgc tggcagcctg ggcgctcctc gtcgttcaca cgacttgggc ggcggacgcc
     1321 gacacgccac cctcggcatc cccagccgcg ggtctccccg gcgtgcaggc ggacgggacc
     1381 gcggatgaca ccgctgcgct ccagcgtgtc ctggaccagg ccggccaggc cgggggccaa
     1441 gtgctcctgc cgggcgcccg gtacaagatc gccggcagtc ttcggatccc gcccggtgtg
     1501 acgctccagg gcgtgagcga atccccggtc tggagcgagc cgctcaaggg atcgatcata
     1561 ctggccaccg ggggacgcga tcaggaggat ggtccggccc tgtttgaact gggccattcg
     1621 tccgccgtgc gcgggctcac ggtcttctac ccggaccagc acccgacgaa catccacccc
     1681 tacgcctgga ccttccacct gcaaggccac gacaacacgg tcgagaatgt cacgttgatc
     1741 aactcgtaca atggcatccg gatcggtccc gaaggcaacg tgcgccatcg catccgcagt
     1801 gtttacggct gcgtgctgcg ccggggcatt ttcgtcgacg cctgcaccga catcggacgc
     1861 atcgacaacg tccagttcca ctgccattgg tggtcggctg ccgcggtcgg gggggactgg
     1921 aagccggtgc acgagttcat gtggaagaac tgcgaagcgt ttatctttgg tcgcacggac
     1981 tgggaatacg tcaacaacac cttcgtctat cctgtcaaca ttggctaccg cttcatccgc
     2041 acccaggcgg gcgcggccaa cggccagctc tcgggcattg gcgccgacga agcccaggtc
     2101 tgcgtgcggg tcgagcatat ccagccgatg ggccttttga tctcgaacgg ccagttcgtc
     2161 tgcatgcacg gcgacacccg cgtgccggtc ctggtcgaga gcacctgtca gggctccgtc
     2221 cgcctggtgg actgcgcgtt ctggggaccc aaccgccaat gcgttgtgtc gcacagccgg
     2281 agttttgtct ccctctccga ttgctacttg agcagcaccg gacgcgcgaa gaatccgggc
     2341 gtcgcgctca tcgaagccga cgggggcaaa ctccaggtgc gcggctgcag cttcggcacg
     2401 gacgaaccgg gcatcgcact gaagaaaggg ctcgagcacg ccatcgtcac tgaaaacaac
     2461 ggcgtgcgcg gcgtggaaat cagaaacgaa atcggcgaca aggcgatcat cgccaacaat
     2521 gagccggagt tgaagtgaga gaagggttca aggaaaccac ggtgacgtgg aagcgagtgg
     2581 tgcggggagg cagggagaat gaccgcggat tgcgcggatg acgcggataa ggaaatcccc
     2641 atccgcgaaa tccgtgaaat ccgtggtcag gactccgtga agtctgcagg ttcacgtatt
     2701 tgctttcctc tctgcgagat cgcactacaa tcggcccggt tcagggcctc atgcaagaat
     2761 ccgcgtggca aacccggagg cagcggatcg atacccggcg acgctccctc tcgcccgcct
     2821 gggagatcgc cccctggcgc gccggtctcg acgtgacgca actcgaccgc tgcgctgtga
     2881 ccgagatccc gaccgcggcg agcgctacga cgtcctcctc accaatccgc ccttcggaac
     2941 aaaaggcgcc aaccaggccc ccgaccgcga cgacttcacc atcgagacca acaacaagca
     3001 gctcaacttc
//
LOCUS       DAOQRO010000391         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_37788_length_3008_cov_4.508974, whole genome shotgun sequence.
ACCESSION   DAOQRO010000391 DAOQRO010000000
VERSION     DAOQRO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37788_length_3008_cov_4.508974"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PKK20_12205"
     CDS             complement(<1..1434)
                     /locus_tag="PKK20_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00693.1"
                     /translation="MKRTIVHAGAALASAWLALSASASVTPQGWWHYGEVLDYYADSS
                     GNNRRFGFAYCSGGGGTTGAAIVPLGAGGPLGTTGHISTSSLYWTPAHYAAAAMWNPW
                     PCSTADCAWNPPATDYVIECWILPEDTGISTTANRTWFFGSGSGDFSQPDRPAGAGGG
                     GVYFAVTNNTELGKSQIGAFVIPNAGQGVPNTVQIGDYVDADTNSWMHIAVVSDNGTN
                     TFYVNGVAKGAPTDKNTIPNGNIFAGGSPGTLPSFRGYLDELRISTFAPGQFQVSDLL
                     LRPAGPSIVEQPKSATVWASGAASFGVVAAKDASLTYQWRRGGANLPGQTGAKLYLAT
                     VTAADNGAKFDCVLTSGGVSVTSAEVTLTVVTPTQGDIDDANAYRNLVKAESGLVAYF
                     PADDCVDATVINVVDNAHNGTLQLGALYDGQTDRALIQRSLAFDADGMVQIPSDPAFE
                     FPSGNGTVEALVYLTRTTIQNQTIFSVA"
     gene            1690..2988
                     /locus_tag="PKK20_12210"
     CDS             1690..2988
                     /locus_tag="PKK20_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV00694.1"
                     /translation="MNRFRISALAALVVLVGSLGAQSPQSTNAPAEPRPVSLDALVEE
                     IRDRHPELAFYRAEVAAARAGVRGARQWENPELSVEVGSKRVWDRGRGATLGDGAVWS
                     VALAQPFEWPGRVALRKAIANQQIEMAELGLAQFQAALANRARTLGRSVVAAQEQADA
                     TDEVAERFQALLAVLVQRDPAGVTPVLDQRIIEAHAITLSRQAAEARRAVQASLIEIN
                     QLRGLPAATPLAIEGALTVATNLPPLSLLLDVAATNNFDIRLRQAELAQQGFQVQLAR
                     NERYPRVTLAPFYAAEKANDEQRIVGLGVSLPLPLWHQNRAGVEVAAARQQQAEASLR
                     TICLEVERQVTGHALALETRLKEMARWRSDAPRQLREAAILADEHYRLGAVPVTTYLE
                     MQTRYLEALEAVLATRAEAIEHRQQLELLLGVALDSLPAR"
BASE COUNT          545 a          940 c          976 g          547 t
ORIGIN      
        1 ggccaccgag aagatcgtct ggttctggat cgtcgttcgc gtcagataga cgagtgcttc
       61 cacagtccca ttgccactcg ggaactcgaa ggccgggtcg ctcggaatct gcaccattcc
      121 atccgcgtca aacgccagag agcgctgaat cagtgcccga tcggtttggc catcatacaa
      181 cgcccccagt tggagagtgc cgttgtgtgc attgtccaca acgttgatca cggtcgcatc
      241 gacacaatca tcggccggga aataagcgac caggcccgat tcggccttga cgagattccg
      301 gtaggcattg gcatcgtcga tatcgccttg agtcggcgtc accacggtca gcgttacctc
      361 cgcactggtc acggaaacgc cgccgctggt caggacacaa tcgaacttgg caccgttgtc
      421 ggccgcggtg acggtggcca gatacaactt ggccccggtc tgcccgggga gattcgcccc
      481 gccgcgccgc cattggtagg tcagcgaggc gtccttggcg gcaaccacgc cgaacgaggc
      541 ggcgccgctg gcccaaacgg tcgcggactt cggttgttca acgatgctgg ggcccgccgg
      601 acggagcagc agatcgctga cctggaactg gcccggagcg aacgtgctaa tgcgcaactc
      661 gtccagatac ccccggaacg agggcagtgt acccgggctg cctccggcga agatgttccc
      721 gttgggaatc gtgtttttgt ccgtgggggc gcctttcgca acgccattca catagaatgt
      781 gttggtgccg ttgtcgctga cgacggcgat atgcatccaa ctgttggtgt ccgcatcgac
      841 atagtcccca atctggacgg tgttcggaac accctggccg gcattcggga tcacgaacgc
      901 gccgatctgc gattttccca gttcggtatt gttggtcacg gcaaagtaca cacctccgcc
      961 acccgcgccg gcgggccggt cgggctgcga gaaatcaccc gaaccgctgc caaaaaacca
     1021 tgtccggttg gccgttgtgc tgatccccgt gtcctcgggc agaatccagc actcgatcac
     1081 atagtcggtt gccggcgggt tccaggcgca gtccgccgtg ctgcatggcc acggattcca
     1141 catggccgcc gcggcgtagt gcgccggggt ccagtagagc gagctggtgc tgatgtggcc
     1201 ggtggtgccc aatggaccgc cggcgccgag cggaacaatg gcggcgcccg tggtgccgcc
     1261 tcctccgctg caataggcaa agccgaatcg ccggttgttg ccgctcgaat cagcatagta
     1321 gtccaagact tccccgtaat gccaccaccc ttgcggtgtg acggacgcgg acgcggacaa
     1381 ggcgagccaa gccgaggcca aggccgcccc ggcatgtacg attgttcgtt tcatgttcat
     1441 gttgagtact ctgttgcgat gacacagcga gctcaaacgg aactcgtgct ttgtgtcaga
     1501 gttactaccc tataccgtct cgaacgattg tcaaagtgga tcaccggcag ccctgcaacc
     1561 acgcaacacg ctcaggatca attcaccttg aattcgcttt actcaaaggg gttcaatgcc
     1621 acgcggaccc atgccgagac accgatgtcg tcggccggtt ccgctcagat cccgatgccg
     1681 attgagacta tgaatcgttt tagaatcagc gctctggccg ccctcgtggt tctggtcgga
     1741 tcgctcggcg cccaatcgcc tcagtccaca aacgccccgg cagagccccg cccggtttcg
     1801 ctcgacgcgt tggtcgagga gattcgagac cgccatcccg agttggcgtt ctaccgcgcc
     1861 gaggtggccg cggcgcgggc gggggttcgc ggggcgcggc agtgggagaa tcccgaactg
     1921 tccgtcgagg tgggaagcaa acgggtttgg gatcgggggc gtggggcgac gttgggggac
     1981 ggcgcggtgt ggtcggtggc tttggcgcag cccttcgagt ggccggggcg ggtggctttg
     2041 cgcaaggcga ttgccaacca acagatcgag atggcggagt tggggttggc gcagtttcag
     2101 gcggcgttgg cgaaccgggc gcgcacgctg ggacggagcg ttgtggcggc ccaggagcag
     2161 gccgatgcca cggacgaagt cgcggaacgt ttccaggcgc ttctcgccgt attggtgcag
     2221 cgcgaccctg ctggagtaac gccggtgctg gaccagcgaa tcatcgaggc gcatgcaatt
     2281 accctgagcc gccaggccgc cgaggcccgg cgcgctgtcc aggccagtct gatcgaaatc
     2341 aaccaactgc ggggcctgcc ggcggccacc ccgctcgcga tcgagggcgc cctgacagtc
     2401 gccaccaacc tgcccccgct ttcgctgttg cttgacgtgg cggcaaccaa caacttcgac
     2461 atccgcctgc ggcaggcgga actggctcag cagggttttc aggtccagtt ggcccggaac
     2521 gaacgctacc cgcgcgtcac cctcgctccg ttctacgcgg ccgagaaggc caatgacgag
     2581 cagcgcatcg tcggtctggg ggtaagtctg cccctgccct tgtggcatca gaatcgggcc
     2641 ggcgtcgaag tggcggccgc ccgccaacag caggccgagg cctcgctgag aacgatctgc
     2701 ctcgaggtgg aacgacaggt gacagggcac gccctcgcac tcgagacgcg actcaaggag
     2761 atggcccggt ggcgcagcga cgcgccccgg cagttgcgcg aggccgcgat tctggccgac
     2821 gagcattacc ggctgggcgc ggtgccggtg acgacctacc tggagatgca gacgcggtat
     2881 ctggaggcgc tcgaagcggt tttggccaca cgcgcggaag cgatcgaaca ccgtcagcaa
     2941 ctcgagttgc ttctgggggt tgcgctcgat tcacttccag cccgctaatg gtctgtgcaa
     3001 aaactaat
//
LOCUS       DAOQRO010000392         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_39050_length_2944_cov_5.966424, whole genome shotgun sequence.
ACCESSION   DAOQRO010000392 DAOQRO010000000
VERSION     DAOQRO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39050_length_2944_cov_5.966424"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..2091
                     /gene="istA"
                     /locus_tag="PKK20_12215"
     CDS             544..2091
                     /gene="istA"
                     /locus_tag="PKK20_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNV00695.1"
                     /translation="MPRRRLSMRKIKEVLRLKWEGGLSHRAIAGSCGIGVTTVREYLV
                     RAARAGLSWPLPEDLDERELEALLFKTPVSAPARKRPLPDWERIHQELKRKGVTLQLL
                     WQEYKQEHPEGYQYTQFCQHYCRWTGRLDVWMRQTHKAGEKMFVDYAGQTMPIVTGST
                     GEVRQAQIFVAVLGASNYTYVEAAWTQSLPEWVMAHTRAFSFVQGCPAIVVCDNLRSA
                     VSKACRYEPDLNPTYCAMARHYGVAVVPARSGKPRDKAKVENSVLNVERHILAPLRDR
                     TFFSLAELNQAIKDLVRRYNERPFQKLEGSRKSLFETLDRPALKPLPQTPYEYAEWKK
                     ARVNIDYHVDADGHYYSVPHELARQQVDVRMTVTAVEIFHNGVRVASHMRSHQKGRHT
                     TVTGHMPESHQAQQGWTPERIVRWARKFGEATAELVALIIESRQHPQQGYRPCLGLLR
                     LGDQFGPQRLEAACVRALAIQAPSYKSVKSILEKELDRRPVPEPAREPVVIDHPNIRG
                     PEYFNSN"
     gene            2167..2925
                     /gene="istB"
                     /locus_tag="PKK20_12220"
     CDS             2167..2925
                     /gene="istB"
                     /locus_tag="PKK20_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNV00696.1"
                     /translation="MLNHPTADKLRELKLTGMLQAWQEQLEMPDSDALSFEERFGLLL
                     DREATERANRRLKSRLKRAKLRQDAALEDIDYTGGRGLDKQLMLSLAGCQWIRERHNC
                     LITGPTGSGKSYLACALAHKACREGHTALYLRVPRLFQDLAIAKGDGRYAQLMNTYAK
                     THLLVLDDWGMAGLTDEQRRDLFEVIEDRYDRGSTLITSQVPVNNWHQVIGDPTLADA
                     ILDRLVHNAYKITLKGESMRKKRKSLTKTPKADT"
BASE COUNT          614 a          963 c          854 g          513 t
ORIGIN      
        1 ttatgcgatg cgattgcccg gttgagacca taagtcctca aggcggccga tgactattgc
       61 gtctattccc tgccgtatga cgtgaaaggt gagtctgtat ggaacggagg cttcaatacc
      121 tggggcacga gacccagtct ggaatgccat gagccgctct catcgggggg ttgcggagct
      181 cagtatcatt tcggcacggc accttcggga atatggttgg ccgctgctgg cacgtgtccc
      241 gcttgcatcc ggcccgtcca ctgcgtattt cggagcaatc cgatcagtga tttcggtttc
      301 atccgcccac ccatttcggt ttattccgat cagtgatttc ggacacgtgc acgtgtccgt
      361 ctcggaataa ccagatcacc cgtttcggcc ccgatcacgg ttccaggcgc gggtccggcc
      421 tcagccggtc cttaatgcat tgcgctaccc gtttcggggc gtgcgcgacg caggacagtt
      481 tctgacgcag gcggtatcgt gtgcctcttt tggccatggt ggccaagagt gaaggaggca
      541 gcgatgccaa gaaggagatt gtccatgcgt aagatcaaag aagtgcttcg actgaaatgg
      601 gaaggcggcc tgagccatcg agccatcgcc ggcagttgcg ggatcggggt cacgacggtg
      661 cgcgaatatc tggttcgggc ggctcgggca ggcttgtcct ggccgttgcc cgaggacttg
      721 gatgaacgtg agctggaggc gctcttgttc aagaccccgg tatctgcacc tgctcgcaaa
      781 cgtcccttgc cggattggga acggattcat caggaactca agcgcaaggg cgtcacgctc
      841 caacttttgt ggcaggagta caagcaggag cacccggagg gctaccagta cacccagttt
      901 tgccaacatt attgccgctg gacgggccgc ctggacgtct ggatgcggca gacccacaag
      961 gccggcgaga agatgttcgt ggattatgcc gggcagacga tgccgatcgt aacggggtcc
     1021 acgggcgagg tgcggcaggc ccagatcttt gtcgccgtgc tgggcgcgag caactacacc
     1081 tacgtggaag ccgcctggac gcaaagtctg cccgagtggg tcatggccca tacgcgcgcg
     1141 ttctcgttcg ttcagggttg tccagccatc gtggtctgcg acaatcttcg gagcgccgtt
     1201 tccaaagcgt gccggtacga accggacctc aacccgacct attgcgcgat ggcccgccat
     1261 tacggggtcg ccgtcgtgcc ggcccggtcg ggaaagcccc gcgataaggc caaagtggag
     1321 aacagcgtct tgaacgtgga gcgccatatc ctggcgccgc ttcgtgaccg gaccttcttc
     1381 tcgctggcgg aactgaacca ggccatcaag gatttggtgc gacgctacaa cgagcggccc
     1441 ttccagaagc tcgaggggtc gcgcaagtcc ctgttcgaaa ccctggaccg gccggccctc
     1501 aagcccttgc ctcaaacccc ctacgaatac gcggagtgga agaaggcgcg cgtcaacatc
     1561 gattaccacg tggacgccga cggccattac tacagcgtgc ctcacgaact ggcccgacag
     1621 caggtggatg tgcgcatgac cgtcacggcc gtcgaaatct tccacaacgg cgtgcgcgtc
     1681 gccagccaca tgcgctcgca tcaaaagggc cggcatacga ccgtcaccgg ccatatgccc
     1741 gaaagccatc aggcccagca aggctggacg cccgaacgca tcgtccgctg ggcgcgcaag
     1801 tttggggagg cgaccgccga gctcgtcgcg ctcatcatcg aatcgcgcca acatccccag
     1861 cagggctacc gcccgtgcct gggcctgttg cgcctgggcg accagttcgg cccccaacgg
     1921 ctcgaggcgg cctgcgtgcg cgcactggcc atacaggccc cctcctacaa gagcgtcaag
     1981 agcatcctcg agaaggaact ggaccggcgc cccgtgcccg aacccgcgcg cgaaccggtc
     2041 gtcatcgacc accccaacat tcgcggcccc gagtacttca acagcaactg aacgggcagg
     2101 ggagtcccga acggaaccgt cttttcccgc gggactcccg ccccttaacc caacaggaga
     2161 ataattatgc tcaaccatcc gacggcagac aaactcagag aactgaaact caccggcatg
     2221 ctccaagcct ggcaggaaca actcgagatg cccgacagcg acgccctctc cttcgaggaa
     2281 cgcttcggcc tcctgctcga ccgcgaggct accgagcgcg ccaatcgccg cctaaagagc
     2341 cgcctgaaac gggccaagct gcgtcaggac gccgcgctcg aggacatcga ctacaccggg
     2401 gggcgcggac tcgacaaaca actcatgctc tccctggccg gatgccagtg gatccgcgag
     2461 cgccacaact gcctcatcac cgggcccacc ggttccggta agagctacct ggcctgcgcc
     2521 ctggctcaca aagcctgccg cgaaggacat accgccctgt atctgcgcgt tccccggctc
     2581 ttccaggatc tcgccatcgc caaaggcgac ggacgctacg cccaactcat gaacacctat
     2641 gccaagaccc acctcctggt cttggatgac tgggggatgg cgggcctgac ggacgaacaa
     2701 agacgcgacc tcttcgaggt cattgaagac cgctacgacc gaggctccac cctcatcacc
     2761 agccaggttc ccgtcaacaa ctggcaccag gtcattggcg accccaccct ggccgacgcc
     2821 atcctcgacc gcctcgtcca caacgcctac aaaattaccc tcaaaggaga atccatgcgc
     2881 aaaaaacgaa aaagcttgac caaaacccca aaagctgata cataaaccca tgtccggcgt
     2941 cgct
//
LOCUS       DAOQRO010000393         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_39465_length_2923_cov_6.164923, whole genome shotgun sequence.
ACCESSION   DAOQRO010000393 DAOQRO010000000
VERSION     DAOQRO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39465_length_2923_cov_6.164923"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..621)
                     /locus_tag="PKK20_12225"
     CDS             complement(166..621)
                     /locus_tag="PKK20_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNV00697.1"
                     /translation="MKQDVAAVEIRGILPANNGVAVFVGNDEKVFVIQVEQQMGAVIG
                     MFLRDTPKERPLTHDLFIHLLTAFGISVERVVITELKNSTYFARLILQQQNELGRKIV
                     ELDARPSDCLALATAQKRPIYVTRSLLDQVEDMSEYLARMSESGGESAD"
     gene            complement(680..>2923)
                     /locus_tag="PKK20_12230"
     CDS             complement(680..>2923)
                     /locus_tag="PKK20_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNV00698.1"
                     /translation="GSPPPKPDQTRSTITRHLAKPVFASRTLSDMESTNGWSLFGPGT
                     MTLSTEHALEGKHALRLRSPTRTEKPGPVAGRPFAETGLRFDVPSEDWSGFNRLSVRV
                     RPHLPGFNVVSLLIKLRSEGTEGQSYTDGGLHFVLLRNHEWNHVVWEIAHLDRRQVRG
                     VELIYRLQGNEPGADDQVQYDFDQLELERVDPDHFLGWAVAPGRFSYNQLGYAPGAVK
                     RAFASGLETNSFSLVDSVSGQAVWRGAVVPGQDPEPAVQVLDFTAWRQPGEYRLQAGD
                     RVSEPFRIGSGLWRHTVESVLDFFHAERCGWAVPGIHEICHRDWLGVNGDLRMVINGG
                     WHDAGDLSQGLVNTAEATYAMFRLAERLRARSAADPSLLPLASRLIDEGRWGLDWILK
                     TRFPDGRRIAWATMDFWTDGILGTVDDVTAQASDAPFDTLQAAAACALAGRLLRAEDP
                     LLAARCLRTAEQDWRRALERLGEPRLELASAAGHAALELFRGTKRREFAEKAIEYAAV
                     VLECQETRTQPWSLPLAGFFYTSPKRERLLHYNHRSHEQAPVMLLADLHQTFPGDSRR
                     AEWSAAIRRYADYLKGAATVTAPYGMLPAGVYRADEPSRPEERAQVRQGMPLDDRHFL
                     RRFPVWGDFRGNLGVQLSQALALGAAARLLEDVEARSLVEAQLDWTLGRNPFAQSLMY
                     GIGHEYAPQYTAMSGDMVGSLPVGIQSRRAGDEPYWPPSNCYNYAEVWVHPAIRWLGI
                     MAELDPD"
BASE COUNT          510 a          991 c          926 g          496 t
ORIGIN      
        1 cccggtgccg ccccgccccg aagggctggg gggctttggc tcgatggtcc cgcggacgcg
       61 ggattgctcc tcggtcacgg accgctgcgg gtatgctcct ttgtcgcgcc tcgcccgcga
      121 gccaaatccc ctccagcaaa atcggacgta atttttgcac agaccctaat cggcgctttc
      181 cccgccggat tcgctcatgc gtgcgaggta ttcggacatg tcctcgacct gatcaaggag
      241 gctgcgcgtg acgtagatgg ggcgtttctg ggcggtggcc agggccaggc aatcgctggg
      301 gcgcgcatcg agttccacga tcttgcggcc cagttcgttt tgctgctgca ggatcaggcg
      361 ggcgaagtag gtcgagttct tcagttcggt gatcaccaca cgctcgacgc tgatgccaaa
      421 tgcggtcagg agatgaatga acagatcgtg cgtcagcggc cgctctttcg gggtgtcgcg
      481 caggaacatg ccgatcaccg cgcccatctg ctgctccacc tggatcacaa agaccttctc
      541 gtcgttgccg acgaacacgg cgacgccgtt gttggccggc aagatgccgc gaatctcgac
      601 agccgccacg tcctgcttca tggggtcaat gtaagagacc ggatgcaaaa gacaagtcca
      661 cgccatgacc cgccccggct cagtccggat cgagttcggc catgatgcca agccaccgga
      721 tcgccggatg cacccagacc tcggcgtagt tgtagcaatt ggaaggcggc cagtagggct
      781 cgtcgcccgc gcgccgggat tgaatcccca ccggaagcga tccgaccatg tcgccgctca
      841 tagccgtgta ctgcggcgcg tactcgtggc cgatgccgta catcaggctt tgggcgaaag
      901 gattccgccc aagcgtccaa tcgagttgcg cctcgaccag gcttcgcgcc tcgacatcct
      961 cgagcaatcg cgccgccgcc cccagcgcca gcgcctggga aagctgcacc ccgaggttcc
     1021 cgcggaaatc gccccagacc ggaaagcggc gcaggaaatg ccggtcgtcc aatggcatcc
     1081 cctggcggac ctgcgcccgc tcctccggcc tggacggctc gtccgcacgg tagactcccg
     1141 ccggcaacat cccgtacggc gccgtcaccg tcgccgcccc cttgaggtaa tcggcatatc
     1201 ggcgtatcgc ggcgctccac tccgcgcgtc tcgaatcgcc ggggaaagtc tggtgcagat
     1261 cggccagcag catgaccggc gcctgctcgt ggctgcgatg attgtaatgc aggaggcgct
     1321 cccgcttcgg cgaggtgtag aagaagccgg caagcggcag actccacggc tgagttcgag
     1381 tctcctgaca ctcgaggacg acggcagcat actcgatcgc cttctccgca aactcgcgcc
     1441 gcttcgtgcc ccgaaacaac tcgagggcgg cgtgccccgc ggcgcttgcc aactcgagtc
     1501 gcggctcgcc cagcctctcg agcgccctcc gccagtcctg ctcggcggtc ctcagacagc
     1561 gcgcggccag gagcgggtct tcggcccgca ggagccggcc cgccaaggcg cacgcggctg
     1621 cggcctggag cgtgtcgaac ggcgcgtcgc tcgcctgagc cgtcacgtca tccaccgtcc
     1681 cgagaatgcc gtcggtccag aaatccatcg tcgcccaggc gatgcgccgt ccgtcgggga
     1741 atcgggtctt cagaatccag tccaggcccc agcgcccctc gtcgatcaac cgcgaggcca
     1801 aagggagcag tgatgggtcg gctgcggatc gggcgcgcag ccgttcggcg agacggaaca
     1861 tcgcataggt ggcctcagcc gtgttcacca ggccttgaga gagatcgccc gcatcgtgcc
     1921 agccgccatt gatcaccatt cgcagatcgc cgttcacccc cagccagtcg cggtgacaaa
     1981 tctcatggat gcccggcacc gcccagccgc accgctcggc atggaagaag tcgagcaccg
     2041 actcgacggt gtggcgccag aggccggacc cgatgcgaaa cggctccgag acacgatcgc
     2101 cggcctggag tctgtactcg ccgggttgcc tccacgcagt gaagtccaac acctgcaccg
     2161 cgggctcggg gtcttggcct gggaccacgg cgccccgcca gaccgcctgt cccgaaacgc
     2221 tgtcgacgag ggagaacgag tttgtttcga ggccgctcgc gaaggcacgc ttgaccgcgc
     2281 ccggggcgta gccgagttgg ttgtaggaga atcgccccgg ggcaacggcc cagcccaaaa
     2341 aatgatccgg atcgacccgt tcgagttcga gttgatcgaa gtcgtactgg acctggtcgt
     2401 cggccccggg ttcgttgccc tggagccggt agatcaactc gacgccgcgc acctggcggc
     2461 gatcgagatg cgcgatttcc cagaccacgt ggttccattc gtggttgcgc agcaacacaa
     2521 agtgcagtcc gccatccgtg tagctttggc cttcggtgcc ctcgctgcgc agcttgatca
     2581 gcagcgagac cacattgaac cccggcaagt gcgggcgcac ccggaccgac agccggttga
     2641 aaccgctcca gtcctccgag ggcacatcga agcgcaaacc ggtttcggca aacggtcggc
     2701 ccgccaccgg ccccggtttc tcggtgcgcg ttggcgatcg caaccggagg gcgtgtttgc
     2761 cctcgagcgc gtgctcagta ctgagagtca tcgtgccggg accgaagaga ctccagccgt
     2821 ttgtgctttc catatccgac agcgtgcgcg aggcaaaaac cggtttggcg agatgcctcg
     2881 tgattgtcga ccgggtctga tcgggcttgg gcggcggcga tcc
//
LOCUS       DAOQRO010000394         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_39575_length_2918_cov_5.866224, whole genome shotgun sequence.
ACCESSION   DAOQRO010000394 DAOQRO010000000
VERSION     DAOQRO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39575_length_2918_cov_5.866224"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1525
                     /locus_tag="PKK20_12235"
     CDS             326..1525
                     /locus_tag="PKK20_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015301924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00699.1"
                     /translation="MDDDWERLHGLQVGVNDSADDPDADTLSNIQEFAANTDPKKADT
                     DDDGLTDGEELATYNTNPLVADTDGDGLTDGREVKTFGSNPLVADTDADGLTDGQEAD
                     VYHCDPTKPDTDGDGYNDGIEVALGSDPNSAASQPREVAVLGQAILGVKEPGDGGLET
                     PYANAGVFANINDGDLLTRVDTFGGGAAPTASYVGILWNQPVTAPVVRLKLSLAIFFD
                     GGWFGPNNVGPGSGGVLSPNYLLEPAVQVSTDHGATWTTVAHTSDYLTALDGHPLPAV
                     DFGAPTTAQATFTLTEPRSNINGIRLLGSEGGTASLGFLGVFELSVEVERPAAGVILV
                     SVARVGDQFRFEFDTQAGANYVVESRDAIATGDWQTLTTVAGDGTRKQVTDTAGAAQR
                     FYRVATQ"
     gene            1615..2793
                     /locus_tag="PKK20_12240"
     CDS             1615..2793
                     /locus_tag="PKK20_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00700.1"
                     /translation="MRIPTVAKLLGCSAAVFLIADAQAQLLFKTKFSALEGYTNGWII
                     GQPSIGAKWENANADWDWCAANEPEYVKLNNGKSWIPEGQTEPYYIVTATNTTALGGG
                     QMMVASDGNYGTNAQTYFWKIDFPKQMTGPITVTWDWQFHCTNEIPADYDPTNNNYNA
                     QLPGFDHGFGLADYANRTADGAYGNPNWVYSECSTGVRLSSRQDCRFNGLGVCGGSGS
                     WFDRGPEFKDGKVLHMKTIMYVANGVDDDLTYMNTYDNFAQRDGENVWQTAKYDGDPF
                     YTDQLGEKYMSEFGMRRCPGEVDPESGINCIMCWMNGNQYSRYVLVSNIRVWGPDGIP
                     TPTLRIDPAPPNKIKLTFTGWLQAADDIEGPWQDVALPSASPMTIDAASAAKFYRAEN
                     "
BASE COUNT          635 a          950 c          874 g          459 t
ORIGIN      
        1 gggcgccggc ggctatctgg acggcggatt gctgctcgaa cccacggtcc aggtccagac
       61 cgcgggcagc gcggagtgga cgaccgtcag tgccacttcg gactatctca tggcgctgga
      121 cggccatggc attggcggag gcggcaatcc caatccaacc tcggtcacgg cgcagttcac
      181 tctcgacacg ccgatcgccg gcgcgaccgg catccgcatc atcggtgaaa acggcggcaa
      241 cgccggcgcg gacgtcaacg gtttcattgg cgtcttcgaa ctggtcgtaa cagccggaat
      301 cgccaacgac tccgacaacg acggcatgga cgacgattgg gagcggctcc acggtctgca
      361 ggtcggcgtc aacgacagcg ccgacgatcc ggatgccgac accctgtcga atatccagga
      421 gtttgccgca aacaccgacc cgaagaaggc cgacaccgac gacgatgggt tgacggacgg
      481 cgaggaactg gccacgtaca ataccaatcc ccttgtggcc gacaccgacg gcgatgggtt
      541 gacggacgga cgcgaggtca agacgtttgg cagcaacccg cttgtggccg acaccgacgc
      601 cgacggcctc accgacggcc aggaagccga tgtctaccac tgcgatccga ccaagccaga
      661 cactgacggc gacggctaca acgacggaat cgaggttgca ctcgggagcg atcccaactc
      721 ggcagccagc cagccccgcg aggtggccgt cctcggccag gcgattctgg gtgtcaagga
      781 gccgggcgac ggaggtctcg aaacccccta tgccaacgcg ggcgtgttcg ccaacatcaa
      841 cgacggcgat ctcctcacgc gcgtcgacac cttcggcggc ggcgcggcgc ccaccgccag
      901 ctacgtcggc atcctctgga accagcccgt cacggccccg gtcgtgcgcc tcaagctgag
      961 cctggccatc ttcttcgacg gcggttggtt cggccccaac aacgtcggac ccggcagcgg
     1021 cggtgtcttg tcacccaatt acctcctcga gcccgcggtg caggtctcga ccgatcatgg
     1081 cgcaacctgg accaccgtgg cgcacacgtc cgattatctg acggccctcg atggacatcc
     1141 gctgccggct gtcgacttcg gagcacccac cacggcccag gccacattca cgctgaccga
     1201 gccgcggtcc aatatcaatg gcatccggct cctgggcagc gagggcggca cggcgagcct
     1261 cggcttcctc ggcgtcttcg aactgagcgt cgaggtcgag cgcccggccg caggcgtcat
     1321 tctcgtctcg gtcgccagag tgggcgatca gttccggttc gagttcgaca cccaggcagg
     1381 ggcaaattac gtggtcgagt ccagggacgc gatcgcgacc ggcgactggc agacgctcac
     1441 aaccgtcgct ggcgacggca cacgcaaaca ggtgaccgac accgccggcg cggcgcagcg
     1501 cttctatcgg gtagcaacgc agtaagcaaa cagaggaatc cgcatgatgc tcgacatcct
     1561 caccctcacc cgtcagtgaa acacaacaaa aacaacacaa cgaaggttat gagaatgaga
     1621 ataccaacgg tggcaaagct cctgggatgc agtgcggcgg tcttcctgat cgcagacgcc
     1681 caggcgcagt tgctcttcaa aaccaaattc tccgctctcg agggctacac gaacggctgg
     1741 atcatcggtc agccgtcgat cggcgccaag tgggagaacg ccaatgccga ttgggattgg
     1801 tgcgccgcca acgagcccga gtacgtcaag ctcaacaacg gcaagagctg gattcccgag
     1861 ggacagaccg agccgtatta cattgtcaca gccaccaaca ctaccgcgct ggggggaggg
     1921 cagatgatgg tggcttcgga cgggaactat ggaaccaacg cgcaaaccta cttctggaag
     1981 atcgatttcc cgaagcagat gaccgggccg atcaccgtca cttgggactg gcaattccat
     2041 tgcacaaatg agatcccggc tgactatgac cccaccaaca ataactacaa cgcccaactg
     2101 cctggcttcg accacggatt cggcctcgcc gactacgcca atcgcaccgc ggacggcgct
     2161 tatgggaatc cgaactgggt ctacagcgag tgcagcaccg gcgtgcgcct cagctcgcgc
     2221 caggactgcc gcttcaatgg actgggcgtc tgcggcggtt ccggaagttg gtttgatcgg
     2281 ggccccgaat tcaaggacgg caaggtgctc cacatgaaga ccatcatgta cgtcgccaac
     2341 ggggtggacg acgatctgac gtacatgaac acctacgaca atttcgccca gagagacggc
     2401 gaaaacgtct ggcaaaccgc aaaatacgac ggagacccct tctacaccga ccaacttggc
     2461 gagaaataca tgtccgaatt cgggatgcgg cgctgcccgg gtgaagtcga tcccgagagc
     2521 gggatcaact gcatcatgtg ctggatgaac ggcaaccagt actcgcgcta tgtcctggtc
     2581 tcgaacatcc gcgtttgggg ccccgacggc atccccacgc cgaccctccg catcgacccc
     2641 gccccaccga acaagatcaa gctcacgttc acaggctggc tccaggcggc ggacgatatc
     2701 gaaggaccat ggcaggacgt cgccctccca agcgcaagtc ccatgaccat cgacgccgcg
     2761 agcgcggcga aattctatcg ggcggagaat tgatctgcag atcgcattcc tcggtgcccc
     2821 tcggtggggc accgaggaga ttcaatcgca cgtaatcagg aggtggtggc tggacccaga
     2881 attgaactgg ggacacaagg attttcagtc ctctgctc
//
LOCUS       DAOQRO010000395         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_39771_length_2909_cov_4.425018, whole genome shotgun sequence.
ACCESSION   DAOQRO010000395 DAOQRO010000000
VERSION     DAOQRO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39771_length_2909_cov_4.425018"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..1781
                     /locus_tag="PKK20_12245"
     CDS             417..1781
                     /locus_tag="PKK20_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00701.1"
                     /translation="MHNRSHEQSQPAGRGRVRRALRWVWRVGLLLVVLVTVVILYWFR
                     LAFYNAFIRFPEEEANRASLRAMRVPVPPLTNWTEYRGVVHSHSEFSHDCEVSFPEIL
                     EALKETRTDFICMSDHCDNGKADFGRQWRGLREGKLFIPGFEMKEGLMPFGVASNIVL
                     DNAWENDRIAQTALANGGVLFYAHPEEPRDWNRPELTGMEIYNTHADFKDEEQALLRI
                     LPDFILNQKRHPDLVFRLMFDRPVENLRRWDDLNRTRHLTGIAGNDCHQNTGVRGVST
                     PEGTLRIEDTSPRTLRDVRLNFLTRWLLRLFFGPLEPGRVLCRVQLDPYARMARHVGT
                     HVLAQELTETAILDALRAGRAFVGFDMLADSTGCQWLAQGESGQAVMGETLDYTSDIR
                     LVARSPLPCRFTVIKDGEPVLQKEGRSIEWTPSGPGKYRVEAELWIRGEWVPWVYANP
                     IALR"
     gene            2099..>2909
                     /locus_tag="PKK20_12250"
     CDS             2099..>2909
                     /locus_tag="PKK20_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00702.1"
                     /translation="MRNRHFRNRAFCRRPWGALQPAAGLACLLFGANAATIPGLFNTG
                     VDASGALLSGGAVDPHWTLIQSADSSAPGPAAYVVRSGYPIPPWLDNGPDSRWIAPQA
                     SQTSGNSPGDYVYRLRFDLKGFEPATATVTFRWSSDNAGVDVRLNGSSSGIVYDGNFG
                     AFSGDFTLADGFVEGINTLDFVVNNAGDSVNPTGFRAELRGTAEPVAPPGTPPAITRQ
                     PVSITVPFRDPAMFTVGVYGSKPMRHQWRLDGQPILLTTNAQYFIPAAVLTN"
BASE COUNT          556 a          970 c          846 g          537 t
ORIGIN      
        1 caattcctgg tctttgcagc cgacaaggaa aagaaaatct tgagcacgat cgaactgggc
       61 gcccccatga acgcgagcgc cgtagcagcg aacggcacgc tgtatgtggc aacgatgaac
      121 cggctgttcg ctgtggcaag gtgatcaaag aagtgtgatc acaaagtggc atccgacgag
      181 ccgattcgcc agacgttcgc ctgttccaag acacgtaccc ggtgccctca ccgggcgata
      241 gaataccaac aactatcaga tgcaccagtg ggcgtaaatc ggatgccctc cccgccgccc
      301 gttccgcgcg gcgaggcgtc tgccactcag aacagttcga gcaacgcgaa ggttgccagg
      361 tcctaccttc gcgttttcgt ttgagccatg cagcaacgtc cggctaaatc atgcccatgc
      421 acaacagatc gcacgaacag tcgcagccag ccggtcgcgg acgcgtgcgt cgcgcgctcc
      481 gctgggtctg gcgggtgggc ttgttgctcg tcgtgctggt caccgtggtc attctctact
      541 ggtttcgctt ggcgttctat aatgccttca ttcgctttcc cgaggaggaa gccaaccggg
      601 cctccctacg cgccatgcgc gttccggttc cccctctgac caactggacc gagtaccgcg
      661 gtgtggtgca cagccactcg gagttctccc acgattgcga ggtctccttc ccggagattc
      721 tcgaggcgct taaagaaacc aggaccgact tcatttgcat gagcgaccat tgcgacaatg
      781 gaaaagccga cttcggacga caatggcgcg ggttgcggga aggcaagctg ttcatccccg
      841 gcttcgagat gaaggagggc ctcatgccct ttggcgtcgc ctcgaacatc gtgctcgaca
      901 acgcctggga aaacgatcga atcgcccaaa cagcactggc caacggcggc gtgctcttct
      961 atgcccatcc cgaggaaccg cgcgactgga atcgcccgga actcaccggc atggaaatct
     1021 acaacaccca cgccgatttc aaggacgagg aacaggctct gcttcgaatc ctcccggact
     1081 tcatcctgaa ccagaagcgg catcccgacc tcgtcttccg gctgatgttc gatcggccgg
     1141 tcgagaacct ccgccgatgg gacgatctca accgcacccg ccatctgact ggcatcgcgg
     1201 gcaatgactg ccaccaaaac accggcgtgc gcggcgtttc gacgccggag ggcacgctgc
     1261 gcatcgagga taccagcccg cgaacgctcc gcgatgtgcg cttgaacttc ctcacgcgct
     1321 ggctgctgcg gctcttcttc ggtccgctcg aacccggccg tgtcctctgc cgcgtccaac
     1381 tcgatcccta tgcccgcatg gcgcgccacg tcggcacgca cgttctggcc caggaactga
     1441 ccgagacggc gattctcgat gcgctgcggg ccggacgcgc ctttgtcggc ttcgacatgc
     1501 tggcggacag cacgggctgt caatggctcg cccagggcga atccggacag gcggtcatgg
     1561 gcgaaacgct tgactacaca tcggacatcc ggctggtggc gcgatcgccc ctgccctgcc
     1621 gattcactgt gatcaaagac ggcgagcccg ttctccaaaa ggagggccgg tccatcgagt
     1681 ggactccgtc cggccccggc aaatatcgcg tcgaagccga actttggatt cgcggcgaat
     1741 gggtcccctg ggtgtacgcc aatccaatcg cgctgcgctg agggctgctc tcccgcggcc
     1801 gcgcatacgc gtcaacctaa ggtctgtgca agaattagtc ccgatttcgc tggaggggat
     1861 ttggccggcc ggcgaggcgc gacgaaggag catagccttg acctctgtga cctcgaagat
     1921 caagggtcac gttgaaggga aatgcgacac cacttggatg cgcgtcaaac cgcaccgacg
     1981 accgcccaag aactcgccat gaaccgcctc gctccagaga atccttgacc ctttgaacgc
     2041 agcaaacaga ctcggcgtgt ttgcggcaag tcttgccggt cgaggattct cgatagcaat
     2101 gcgcaaccga cacttccgga acagagcttt ttgtcgccgg ccctggggcg ccctgcaacc
     2161 ggccgcgggc ctcgcctgcc tccttttcgg ggcgaacgct gcaaccattc cgggcctctt
     2221 caacacgggc gtcgacgcca gtggcgcatt gctctcgggc ggagcagttg atccgcattg
     2281 gaccctcatc cagagcgccg actcgagcgc gcccgggccg gcggcctacg tggtgcggtc
     2341 gggctatccg attccccctt ggctggacaa cggacccgac tcgaggtgga tcgcgcctca
     2401 ggccagtcag acgagcggca attctcccgg agactacgtc tatcggctcc gctttgacct
     2461 caagggcttc gaacccgcaa ccgcaaccgt cactttccgg tggtcctccg acaatgcggg
     2521 ggtcgacgtg cgcctcaacg gctcgagcag cgggatcgtt tacgacggca atttcggcgc
     2581 attctcaggg gatttcaccc ttgccgacgg attcgtcgaa ggcattaaca cgctcgattt
     2641 cgtcgtcaat aacgccggcg acagcgtcaa ccccaccggt tttcgcgcgg aactgcgcgg
     2701 cacggccgag cctgtcgcgc cccccggcac gccgccggca attacgcgcc aaccggtctc
     2761 gatcacggtc ccctttcgcg atccggctat gttcacggtc ggggtctatg gcagcaagcc
     2821 gatgcgccat cagtggcgtc tggacgggca gccgatcctc ctcacaacca acgcccagta
     2881 tttcatcccc gccgccgtcc tgaccaacg
//
LOCUS       DAOQRO010000396         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_40071_length_2897_cov_4.322660, whole genome shotgun sequence.
ACCESSION   DAOQRO010000396 DAOQRO010000000
VERSION     DAOQRO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40071_length_2897_cov_4.322660"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..580
                     /locus_tag="PKK20_12255"
     CDS             113..580
                     /locus_tag="PKK20_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00703.1"
                     /translation="MNPTSMIQMNPSLCVRQIQGNPYTLIRGWRPLVLDRSGVVDGGR
                     EWPAPIEPVAGTGCERSRRTVGAKALVLAMVVLFAAVVNLSLWGASRVDQQRRTYRLA
                     QQLRKSEALNQLLAVRNRSVNAFLALSEARVSSVDAIAASGQSNSKSLRRNKI"
     gene            629..2476
                     /locus_tag="PKK20_12260"
     CDS             629..2476
                     /locus_tag="PKK20_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV00704.1"
                     /translation="MNTTRQYRVLALMGVVVSVFFGIVALRLVDIQVWRHDELRVKAE
                     RFTRTCRYSEPWRGEVLDCRGRTLATGLPVKTVYADLTVCSNRFEEVAAVIARPLGMT
                     QDRVAEVMRRAAQGNGPDSSRAAKAILLQRSLPLWGWENLTNALAQADFGLPSEPANR
                     RQRLARERLRRRAVFARDDQVRVYPLTNVLGQVLGFVQPGPDGVGLKGVAGLELSLDS
                     LLAGVRGVCVSEQDAAGRELPFARSQWTPAVDGSSVELTIDAAIQQSVERILADAVMR
                     HEPRCASVIVLRPKTGEILAWASSPPANPVRPAEGPIDIWRIYGLSDQLEPGSVLKVL
                     VVAAAFEHGSATPDLQYFCEHGRMMFQGGSLRDHAPYGTLTVRDIIAKSSNIGAAKIA
                     LGVGPERLREVLLRFGLGQRTVLPLLGETPGYLPSADKLTRLVLARAAIGQGLAVSQL
                     QLTLAVGALANNGWLMRPLLVKEVRAPDGKPSFQASPVAVRQAVRPTVAQAVLELMRG
                     VIRPGGTGTAGQPRLYSAGAKTGTAQKANQHGYMRGAYTSWFVGFAPATDPELVVSVM
                     FDEPRRGYYGGEVCAPLFGAVVDEVGPRLGIRADLDTGRRVGNLASRSQ"
     gene            2503..2865
                     /locus_tag="PKK20_12265"
     CDS             2503..2865
                     /locus_tag="PKK20_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNV00705.1"
                     /translation="MNLKTAILLCAVGSLAAIGLVSIDSTMVDRGDPNRLRTCAWALA
                     LGGGLGLAAALIDYRRLSRHGLPRILLGLSLAALALVLVPGIGEEINGARRWLRCGGQ
                     PSEFAKVAVLVWLAACRT"
BASE COUNT          476 a          863 c         1015 g          543 t
ORIGIN      
        1 ccctgttgct cgggttggtg aatcgcttcg agatatggaa tccgaagcgg tatgccgagt
       61 tccaagcaga aagcaaacgc ctggcctccg aggcctacaa gcagatcgca ctatgaatcc
      121 gacctcaatg atccaaatga accccagcct ctgcgtccga cagattcagg gcaatcccta
      181 cactctcatc cgcggatggc ggcctttggt gctggatcgg tcaggggtgg tcgacggggg
      241 gcgcgaatgg cccgcaccga tcgagcccgt ggctggcacg gggtgcgaaa ggtcgcggag
      301 aacagtcggg gccaaggcgc tggtgctggc gatggtcgtg ttgttcgcgg cggtggtgaa
      361 tctgagtctg tggggcgcct cccgcgtcga ccaacagagg aggacctatc ggctggctca
      421 gcaactgcgc aagtccgaag ccctgaacca actgctggct gtccggaatc gcagcgtgaa
      481 cgcgtttctg gcgctgtcgg aggcccgagt cagttcggtc gatgccattg cggcatccgg
      541 ccaatcgaat tccaagtcgt tacgccggaa taagatatga ttcgtttgag catcgggacg
      601 ttacaccctc cacccatgat cgaccacaat gaacaccacc cgccaatacc gcgttctggc
      661 gctgatgggc gtcgttgtca gcgtcttctt cgggattgtc gcgctccggc tcgttgacat
      721 tcaggtctgg cgacacgacg agctgcgggt caaggcggag cgcttcaccc gcacgtgccg
      781 ttactcggag ccgtggcggg gcgaggtgct cgattgtcgg ggacgaacgc tggcgacggg
      841 tttgccggtg aagacggtgt acgccgatct gacggtttgc tcgaatcgct tcgaggaggt
      901 ggcggccgtg attgcgcggc ctttgggcat gacgcaggat cgagtcgcgg aggtgatgcg
      961 ccgagccgcg cagggcaacg gcccggattc ctcccgggca gccaaggcga ttctgctcca
     1021 gcgatctctt ccgctctggg gctgggaaaa cctgacaaat gccctggcgc aggccgactt
     1081 tggactgccg tcggagccgg cgaatcgtcg gcagcgattg gctcgggaac gcctccgacg
     1141 gcgcgcggtc ttcgcccgtg acgaccaggt gcgtgtgtat cccttgacca acgtgctggg
     1201 ccaggttctt ggttttgtgc aaccgggtcc ggacggcgtc ggcctcaagg gcgtcgcggg
     1261 tctcgagctg agcctcgatt ctctgctcgc cggggttcgg ggtgtgtgcg tgtcagagca
     1321 ggacgccgcc ggtcgggaac tgccctttgc gcggagccaa tggacgccgg cggtggatgg
     1381 cagcagtgtc gaactgacga tcgacgccgc gattcagcag agcgtcgagc ggatactggc
     1441 cgacgctgtg atgcgccacg aaccgcgctg cgcgtcggtg atcgtcttgc gtccgaagac
     1501 aggcgagatt ctcgcctggg catcgagtcc gccagcgaac ccggttcgtc ctgccgaggg
     1561 accgatcgac atttggagga tctacggact cagcgaccag ctcgaaccgg gatcggttct
     1621 gaaggtgctg gtcgtggcgg cggccttcga gcacggttcc gccacgccgg accttcagta
     1681 cttctgcgaa cacgggcgga tgatgtttca aggcggttca ctgcgagacc atgcgccgta
     1741 tggaaccctg accgtgcggg acatcatcgc caagtcgtcg aacatcggcg cggccaagat
     1801 cgctctcggg gtggggcccg aacgcctgcg cgaggttctc ctgcgtttcg gtctggggca
     1861 gcggacggtt ctgcccttgc tgggggagac gcccggatac ctgccatccg ccgacaagct
     1921 cacgcgactg gtcctggctc gcgccgcgat cgggcagggg ttggccgtga gccagttgca
     1981 gttgaccctg gcggttggag cgctggcaaa caacggatgg ctcatgcggc cgctcctggt
     2041 gaaggaggtt cgcgctccgg acggcaagcc gagctttcag gccagcccgg ttgcggtgcg
     2101 acaggcggtg cgcccgacgg tggcgcaggc ggtgctcgaa ctgatgcgcg gcgtgatccg
     2161 gccgggggga accggcacgg cgggccaacc caggctctac tctgcgggcg ccaagaccgg
     2221 gaccgcgcag aaggccaatc aacacggcta tatgcgtggc gcctacacct cctggtttgt
     2281 tggattcgcc cccgcaacgg accccgagct ggtcgtttca gtcatgtttg acgaaccgcg
     2341 ccgcggctat tacggcggcg aagtctgcgc gccgctgttt ggcgcggtgg tggacgaggt
     2401 gggtccccgt ctgggcatcc gcgccgacct cgacacgggg cgtcgggtcg ggaacctggc
     2461 gagccgcagt cagtaataac gaagcgcgca ccgcgtcgag tcatgaacct caagacagcc
     2521 attctcctct gtgcagttgg cagcttggcc gccatcggct tggtttccat cgacagcacg
     2581 atggtcgatc gaggcgatcc gaaccggttg cgaacgtgcg cctgggcgct ggcgcttggc
     2641 ggcggccttg gtctggctgc cgccctgatc gactaccgtc gcctgagtcg gcatgggctg
     2701 cctcggattc tgcttggatt gagcctggcc gcgctggcgc tggtcctggt tccgggaatc
     2761 ggcgaagaga tcaacggcgc gcgacggtgg ttgcgatgcg gcggtcagcc atcggaattc
     2821 gccaaagtgg cggtgctggt ctggctagca gcctgtcgga cttagatcga gaagcaagtg
     2881 agggtgatgg tggtagg
//
LOCUS       DAOQRO010000397         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_41168_length_2848_cov_5.475474, whole genome shotgun sequence.
ACCESSION   DAOQRO010000397 DAOQRO010000000
VERSION     DAOQRO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41168_length_2848_cov_5.475474"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKK20_12270"
     CDS             <1..695
                     /locus_tag="PKK20_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV00706.1"
                     /translation="HWSVVDAIAASDNAAAREAWERVAQWYWYPLYAFARRSRVGPED
                     AEDLTQSFFAHILEKNVLAKASKERGRFRSYLLCAFKFHLRHQRRQARTKRKGGHVAV
                     VPLEMKSAEERYAFEPPDCRDPERLYERRCALIVLERGVDRLRQEFVQKERRLVWERL
                     HPLLDGDPPSGGYAQIAADLGLTESNVKVTVHRMRRRLGEFVREELSRTVSNAAELEE
                     ELEHLKAVLSAD"
     gene            972..>2848
                     /locus_tag="PKK20_12275"
     CDS             972..>2848
                     /locus_tag="PKK20_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV00707.1"
                     /translation="MKRKPTHCPHCGRRLPADSPDGHCPACLAAAAVSMAKEEADGDV
                     RDTGLDGEAVESLRIWIKGYELFEELGRGGMGVVYRARQIEARRLVALKVLDRGLVPP
                     GDSSERFFREGRIVASLDHPGIVRLHDIGSDRGHPFFAMELIEGRSLDTVVREGPLPA
                     EPAARILTRIARAIAYAHERKVIHRDLKPSNVIIDAKGEPHVTDFGLARRLDASAALT
                     RTHQNLGTPAYMAPEQVSGAQGPPEPRSDIYSLGAMLYHLLTGHPPFQADTLEAILLQ
                     VVERDPVPLRRLNPNVPRDLEAICLKCLEKEPSHRYPAASALADDLDRFLDHSPVRAR
                     RLTFFHRLWRHSRRRPAIAALVATSMAAVLAGIAGVIWHWHQIEKARADTEQANARLQ
                     TLVSRLRFEHADNLARAGELRAALPHLALLLREAPLHPVTPARILSLLNSFNYFLPLS
                     PPISVAATPNSADLSPDGRLLALTGEGRAVHVIDLERGLPVTPPLRYDKTVTRLAFSP
                     DGRWLAASSLDGTARLWNLTNDQPVCPPIEHPGNVFFLDFAPDGRRLATTCEGRAGEL
                     PGWHWVFVWDLPDGKPACPPHTNRCWFHTVRFSPDGRLIAAASTDRNAHILEAATGRS
                     LG"
BASE COUNT          502 a          955 c          900 g          491 t
ORIGIN      
        1 cacattggag cgtcgttgat gccattgcgg cgagcgacaa cgcggcggcg cgcgaggcgt
       61 gggaacgagt cgcccagtgg tactggtatc cgttgtatgc gtttgcccgc cgaagccgcg
      121 tggggcccga ggacgcggag gacctgacgc aatcgttctt tgcgcatata ctcgaaaaga
      181 acgtcttggc gaaagcctcg aaggagcgcg gtcggttccg cagttacctc ctgtgcgcct
      241 tcaaattcca ccttcggcac caacgccggc aagcgcggac caagcgcaag ggcggacacg
      301 ttgccgtcgt gcccctcgaa atgaagtcgg ctgaagagcg gtacgccttc gagccgccgg
      361 attgccggga cccggagcgg ttgtacgagc ggcgctgcgc gttgatcgtt ctcgagcgcg
      421 gtgttgaccg cttgcggcag gagttcgttc aaaaggagcg ccggctggtg tgggaacggt
      481 tgcacccgct cctggacggc gatccacctt ccggaggcta cgcccaaatc gcggcggacc
      541 tgggcctgac cgaatcgaac gtcaaggtga ccgtgcatcg gatgcgccgt cgtttgggtg
      601 agttcgttcg tgaggagctc tcgcggacgg tctcgaacgc cgccgaactc gaggaagaac
      661 tcgaacatct gaaggcggtg ttgagcgccg attagaatcc ttgaccggtg aaacctgctg
      721 caaccaacgc gggttagaca agcgaggact tcggcgaact gagccgggcc gcgcgccaca
      781 gaaatgcggg gaaacacggc gactgctcat ggaaagaccg gtttcttctt tgggcagcca
      841 aagtcaccat ccagggcctc gtgtaactcc gccctccgag agggcggtgg ttcatggaga
      901 tcgggactgt aaccttcttg aacggacggc agaggaaaag gtgcatcgtg tcctgaactc
      961 tggagtctgg catgaagaga aagccaaccc actgtccgca ctgcggccgt cggctcccgg
     1021 cggattcgcc tgacggccat tgtcccgcct gcctggccgc ggccgccgtg tccatggcta
     1081 aagaggaggc cgacggcgac gtccgggaca ccgggttgga cggggaagcc gtcgagtcgc
     1141 tgcgcatatg gatcaaaggg tacgaactgt tcgaggagtt gggcagaggc gggatgggag
     1201 tcgtctaccg ggcgcgccag atcgaggcgc gccggcttgt ggcgctcaag gtgcttgatc
     1261 ggggcctcgt tccgccgggc gactcgagcg agcgcttttt ccgggaaggc cggatcgtcg
     1321 caagcctcga tcatcccggc atcgtccggc tgcacgacat cggcagcgac aggggacatc
     1381 cattctttgc gatggaactg atcgaggggc ggagtcttga caccgtcgtg cgggaagggc
     1441 cgttgcccgc ggaacctgcg gcccgcattc tgacccggat cgcccgtgcg atcgcctacg
     1501 cccacgagcg gaaggtcatc caccgcgacc tgaaaccgtc gaacgtcatc atcgacgcca
     1561 aaggcgaacc gcatgtgacc gatttcggcc ttgcccgacg gctggacgcc agcgcggcgc
     1621 tcacgcgcac ccaccagaac ctgggcacgc ccgcctacat ggcgcccgaa caggtcagcg
     1681 gcgctcaggg gccgccggag ccgcgcagcg acatctattc actgggggcc atgctctacc
     1741 atcttctgac ggggcatccg cctttccagg ccgacaccct cgaagccatc ttgttgcagg
     1801 tggtcgaacg cgacccggtt ccactccgcc gcctcaaccc gaatgtcccg cgcgatctcg
     1861 aggccatttg cttgaaatgc ctcgaaaagg agccgtcgca tcgctacccc gccgcctccg
     1921 cgctggccga tgacctcgac cgcttcctcg atcacagccc agttcgcgca cgtcggctga
     1981 cgttcttcca tcggctctgg cgacactcga ggcggcgtcc ggcgattgcc gcgctcgtgg
     2041 cgacgtcgat ggccgccgtc ctcgcgggca ttgcgggggt gatctggcac tggcatcaaa
     2101 tcgaaaaggc ccgggccgac accgagcagg ccaacgcccg gctccaaacc ctggtgagcc
     2161 gcctccgatt cgaacacgcc gacaacctgg ctcgagcggg cgaacttcga gcggcgctgc
     2221 cacatctggc cttgctcctg cgcgaagccc ccctccaccc cgtcaccccg gcgcgcatcc
     2281 tcagtctcct caattcgttc aactatttcc tgcccttgtc cccgcccatc tccgtcgcgg
     2341 cgacaccgaa ctccgccgac ctgagtccgg acggacggct cctcgcactg accggcgagg
     2401 gccgtgccgt gcacgtgatc gatctcgaac gcggcctgcc cgtcacgccc cctctgcgct
     2461 acgataagac agtgacgcgg ttggcttttt cgccggacgg acgttggttg gctgcgagtt
     2521 ccctggacgg aacggcacgg ctttggaatt tgaccaacga ccagcccgtt tgcccgccga
     2581 tcgaacaccc cggcaacgtg ttcttcctcg atttcgcccc cgacggccgg cgcctggcca
     2641 cgacgtgcga aggacgcgcg ggcgaactcc ccggctggca ctgggtcttt gtctgggacc
     2701 tcccagacgg aaaacccgcc tgcccgcccc ataccaatcg ctgttggttc cacaccgtcc
     2761 ggttcagtcc ggacggtcgc ctgatcgcgg ccgcttcaac cgacagaaac gcccatatcc
     2821 tcgaagccgc caccggacgc tcgttggg
//
LOCUS       DAOQRO010000398         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_42084_length_2808_cov_5.792953, whole genome shotgun sequence.
ACCESSION   DAOQRO010000398 DAOQRO010000000
VERSION     DAOQRO010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42084_length_2808_cov_5.792953"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKK20_12280"
     CDS             complement(<1..349)
                     /locus_tag="PKK20_12280"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00708.1"
                     /translation="MDSDPAKREVGDLLLAAGQLTQDQLETVRRRQQRWRVSQHSAIL
                     SLSYASEEDTYRALATLHNIEFIDLSKTSLPPNFKELVPVAVMLNHRVVAVAAENDTL
                     VLAFSEPPRQAEVS"
     gene            complement(353..853)
                     /locus_tag="PKK20_12285"
     CDS             complement(353..853)
                     /locus_tag="PKK20_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00709.1"
                     /translation="MLGALERTIWFSALLLAGLAILLALVLFGFGGGLPDLKSPAGSR
                     ASANPPLVEPIPDLFATSAVPALETSSNAINPFFTLYFQPPAPPPTKKVPLLYLGCVE
                     SSTRVLRAYVRVADKLQILPLGEKVIADYAIQNISLKALVLTNTTGQTSVFGFKTNAT
                     IEVPAN"
     gene            complement(877..1716)
                     /locus_tag="PKK20_12290"
     CDS             complement(877..1716)
                     /locus_tag="PKK20_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00710.1"
                     /translation="MASRAHSSRLPIATALAGLVLVLYYASVFKPLSRRVDDLDGPLT
                     NLWHSFVTTNRTHEACAGLDFTLVDRRVEELRGALKHLESAQIAVQTRLKLPPEVNAR
                     LAEPFQLIDFQNDRSRQAEALTRLAKEKGVACEPAVLNGLPEYSVDLVDPRLLWPRLY
                     FANQLLLAAVHSKVAGLRSLTQLPSISHRKDSVGDGLLEELPMRIELFGPTPAVAQFV
                     TSLPLRGEDLNLVGLDTLLTNKPVFFAGRILARKHSPERPEDVLLELTVSGWVVAAGG
                     PSE"
     gene            complement(1719..>2808)
                     /locus_tag="PKK20_12295"
     CDS             complement(1719..>2808)
                     /locus_tag="PKK20_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00711.1"
                     /translation="SKDVLAPPTGSPACVQAVAKWYADLQRAVVEWMDDYPELRIPWQ
                     SLPVYLCGGGASQPGLFDHLNRLGSLRFQPWPKCPGVDPDLAMDQYWVAYGAARAALG
                     RARSTVSLLPPELKSLARRHRRWEWLQAANIALLMLIAAALGLATWQQSKLLERKELL
                     IAKTRAALLSADTVVHLAQRLDAGYAALRPVFQRQQHSLDTIAALAAIRQIRTNDDFW
                     LALFADAASYATAPILPPPTNAVIESAPPPSTNAFPPRREFIAELCIPQQGDAARRIL
                     SQVVSDLKQKPIFERADALPPERKRAVVDPRVVITNRLFAIAMEVASGRTNGVIDALL
                     QPNRALPPKRPGPAPRPRDGASPGATEP"
BASE COUNT          494 a          754 c         1070 g          490 t
ORIGIN      
        1 tgctgacctc ggcctggcgt gggggctcgg agaaggcgag gacgagcgtg tcgttttcgg
       61 cggcgacggc gacgacgcgg tggttgagca tgaccgcgac ggggaccagt tccttgaaat
      121 tggggggcag ggaggtcttg gagaggtcga tgaactcgat gttgtggagt gtggccagcg
      181 cgcggtaggt gtcttcttcg gaggcgtaac tgaggctcag gatggcggag tgttgcgaga
      241 cgcgccagcg ctgctggcgg cggcgcaccg tttcgagctg gtcctgggtc aattggccgg
      301 cggccaggag cagatctccg acttctcgtt tggcaggatc agaatccaca ggtcagtttg
      361 ccggcacttc aatggttgcg ttggtcttga aaccgaacac gcttgtctgc cccgtcgtgt
      421 tggttaggac cagggccttg agcgagatgt tctggatcgc gtagtcggcg atgacctttt
      481 cgcccaaggg caggatctgg agcttgtcag cgactcgcac gtaagctcga agcacgcggg
      541 tggacgactc gacacagccg aggtagagga gggggacctt cttggtcggc ggcggcgcgg
      601 gtggttgaaa gtaaagcgtg aagaaggggt tgatcgcgtt gctcgaggtc tcgagcgccg
      661 gcaccgccga ggtggcgaac aggtccggga tgggctcgac gaggggcggg tttgccgaag
      721 cgcgcgaccc ggcgggcgat ttgagatccg gaagcccacc gccaaaaccg aagaggacta
      781 gcgcgagcaa gatcgccaga cccgccagta ggagggctga aaaccagatt gttctttcca
      841 gggcgccgag catgcagcga gttcttgtga gatcggtcac tccgacggtc cgccggcggc
      901 gacgacccaa ccagaaaccg tcagttcgag caagacatcc tccggacgtt ccggcgagtg
      961 tttgcgggcg agaatccggc cagcgaagaa gaccggtttg ttggtgagga gagtgtccag
     1021 accgacgagg ttgaggtctt cgccccggag ggggaggctg gtcacgaact gggcgaccgc
     1081 gggggttggg ccgaacagtt cgatgcgcat gggcagctct tcgaggagtc cgtcgccgac
     1141 gctgtccttc cgatggctga tcgaggggag ttgggtgagg ctgcgcaggc cggcgacctt
     1201 cgagtgcacg gccgccagca ggagttggtt ggcaaaatag agacgcggcc agagcaggcg
     1261 cggatcgacg agatcgaccg agtactcggg caggccgttg agaacagcgg gctcgcaggc
     1321 gacgcctttc tccttggcga ggcgcgtgag ggcttcggcc tggcggctgc gatcgttctg
     1381 gaagtcgatg agttggaacg gttcggcgag tcttgcgttg acttcggggg gcaacttgag
     1441 gcgtgtttgg actgcgattt gggccgactc gagatgcttc agagcgccac gaagctcctc
     1501 gacccgccgg tcgaccaggg tgaaatcgag gccggcgcat gcctcgtgtg tgcgattggt
     1561 cgtgacgaaa ctatgccaga gattggtgag cggcccgtcg aggtcgtcga cccggcgcga
     1621 caacggtttg aacaccgaag cgtaatagag caccagcacc agccccgcca gggcggtcgc
     1681 gatcgggagt cggctcgagt gcgcgcggga agccatggtt acggttcggt agcgccgggc
     1741 gatgctccgt cgcgaggtcg cggcgctggt ccgggacgtt tcggagggag ggctcgattc
     1801 ggttggagca gggcgtcgat cacgccgttg gtgcggcccg acgccacctc cattgcaatg
     1861 gcgaagaggc ggttggtgat gacgacgcgt ggatcgacca cggcgcgctt gcgctcgggt
     1921 ggcaacgcgt cggcgcgctc gaagatcggc ttttgtttga gatcgctgac cacctgactg
     1981 aggatgcggc gggcggcatc gccctgttga ggaatgcaca gttcggcgat gaactcgcgc
     2041 cgcggcggaa aggcgttggt cgagggcggg ggcgcggatt caatcaccgc attggttggc
     2101 ggaggaagaa tgggagcggt ggcatagctg gcggcgtccg cgaacagggc gagccagaaa
     2161 tcgtcgttgg tgcggatttg gcggatggcg gccagggccg cgatggtatc gagagagtgc
     2221 tgctggcgtt ggaagaccgg gcgaagcgcg gcgtagccgg cgtccaggcg ctgggcgagg
     2281 tggacgacgg tgtcggcgga gagcagggcg gcgcgggttt tggcgatcag gagttcctta
     2341 cgttcgagca acttgctttg ttgccatgtg gccaggccca gggcggccgc gatgagcatc
     2401 aggagcgcaa tgttggcggc ctgcagccat tcccaccggc ggtggcgccg cgccaggctt
     2461 ttgagttcag gcggcagcag cgaaacggtt gatcgcgcgc ggcccagggc agcccgcgcg
     2521 gctccgtagg caacccagta ttgatccatc gccaggtccg gatcgacacc ggggcacttg
     2581 ggccagggct ggaaacgcag gctgcccagg cggttcaaat ggtcgaacaa gccgggctgg
     2641 gaagcgcctc ccccgcagag gtacactggc agcgactgcc acggaattct caactcgggg
     2701 tagtcgtcca tccactcgac gactgcgcgc tgcagatcgg cgtaccactt ggcgacggcc
     2761 tgaacgcatg cgggcgatcc ggtgggaggg gcgagcacat ccttcgac
//
LOCUS       DAOQRO010000399         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_42741_length_2779_cov_6.970999, whole genome shotgun sequence.
ACCESSION   DAOQRO010000399 DAOQRO010000000
VERSION     DAOQRO010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42741_length_2779_cov_6.970999"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..742
                     /locus_tag="PKK20_12300"
     CDS             284..742
                     /locus_tag="PKK20_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV00712.1"
                     /translation="MRVTGTVDALLKAKGNVVWSISPDAMVYDAISMLAEKNVGALMV
                     MEDETLVGIFSERDYTRKVALLGKNSRTTRVREILSSEIVPVSLEATVEDCMRLMTEH
                     RIRHLPVVERNQVLGVLSIGDLVNWIITAQQAKIDQLENYIAGSYPSTTE"
     gene            complement(861..2678)
                     /locus_tag="PKK20_12305"
     CDS             complement(861..2678)
                     /locus_tag="PKK20_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV00713.1"
                     /translation="MDSQAIHRPRNLDWKRAAALLYGDWGTSKAYVIGLAFAAAGHAS
                     LPIIGAVCVLTALVGYNYILVCRHFPDGGGVYSAARDQSRLLAVIGALLLVANFLVTA
                     ALSCNSAMHYFGVSAGQVAAATVLVILIIGGINFFGPKHTGSLAVGLMVPMVVVVVAL
                     ILLSLPRLSTAHLEPPRGGIARNWQFFVATILALSGVEAIANLTGVMKLDPGATMDKP
                     CVSKTARKAILPVAAEVVLGTALLGWAMLSLSGAHAPDPVAHRDYMMRFLGQEYGTLM
                     FNAGVGKAIGAVVGLVVGLLLLSAVNTAIAAQIGLIYMLARDGEMPRFFTRLNAYGVP
                     WWPWTMATLLPILIVLVTGADIESLANLYAIGVVGAITVNLGSCTFNRSLRMAWYERG
                     IMGATFAVLFAVEITIAKVNHGALFFAICLLGVGLSLRTITQRRAGLRTVTLRREIAA
                     RVAPETAPEFELQLETGQTILVAARGLTPVLRFALEEARLRQGALYVLYVKELAVAMP
                     GLLQTAERPRWQDDPAAAAIMSSMLHLGARHNVQVVPLYAVSDDPANSILDLAATLGV
                     DILMLGSPQRRRLVQLLKGNVVTEVARNLPENIQLVIHG"
     gene            complement(2704..2778)
                     /locus_tag="PKK20_12310"
     tRNA            complement(2704..2778)
                     /locus_tag="PKK20_12310"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2744..2746),aa:Met,seq:cat)
BASE COUNT          588 a          894 c          840 g          457 t
ORIGIN      
        1 gcaatccagt tggaaacctt gtagcaagtc ccatggtatt gttcgatgtc cgagattctt
       61 cagaaaggaa acgggcggat cggcactgaa cagttcgtgg tttgtcgaga tcatcgcacg
      121 cctactacgc accaaaccac gccaattgac aagcgttatt tccaacctca acaacagcaa
      181 cccttcagga atttaaatga aataaccgtg gccacctccg tggcgctgtc ggggaggggc
      241 ttgacttctc cgaccaacgg atcaatcctc gcgctcgaac accatgagag tgacaggcac
      301 agtcgacgcg cttctcaagg caaagggcaa cgtcgtgtgg tcgatctcgc ccgatgcgat
      361 ggtctacgat gccatcagca tgctagctga gaaaaacgtc ggggctctga tggtcatgga
      421 ggatgagaca ttggtcggga tcttttccga gcgcgactac acgcgcaagg tggcgctgct
      481 gggcaagaac tcgaggacga cccgggtgcg ggagattctc tcgagtgaaa tcgtgcccgt
      541 ctcgctcgaa gccacagtcg aggactgcat gcgcttgatg accgagcacc gcattcggca
      601 tctgccggtc gtcgagcgga accaggtgct gggtgttctc tcgatcggcg acctggtcaa
      661 ctggatcatc acggcccagc aggccaagat cgatcaactc gagaattata tcgccgggtc
      721 ctatcccagc acgacggagt aaggcgcccc attcgatttt cccgcgcgat ccgcattacc
      781 ggttcctgcc tcctgaaccg cggagccgtt ctcttgcgaa gtggggccgg cgtgtgcgcc
      841 gacggggcgg aacatgcggg tcagccgtgg atgaccaact gaatgttctc gggcaggttc
      901 cgcgcaacct cagtcaccac attccccttg agcaactgga ccagccggcg cctttgcggc
      961 gaaccgagca tcagaatgtc cacgcccaga gtggccgcca gatccagaat ggaattggcc
     1021 ggatcgtcgc tgaccgcgta gagcggaacc acctgaacgt tgtgacgagc cccgaggtgc
     1081 agcatcgaac tcatgatcgc cgcggccgcc ggatcgtcct gccagcgggg ccgctcggcg
     1141 gtctggagca ggccgggcat cgccacggcc agttccttga cgtagagcac atagagcgcc
     1201 ccctgacgca accgcgcctc ctcgagggcg aaccgaagca ccggggtgag cccgcgcgcg
     1261 gcaaccagga tggtctgtcc cgtctcgagc tgaagctcga actcaggcgc ggtctcgggc
     1321 gcgactcggg cggcgatctc ccggcgcagc gtcaccgtgc gcagcccggc ccgccgctgc
     1381 gtgatggttc gaaggctcag gcccacgccc aacaggcaga tggcgaagaa gagagcgccg
     1441 tgattcacct tcgcgattgt gatttcgacg gcaaacagaa cggcgaaggt cgcgcccatg
     1501 atcccgcgtt cataccaagc catgcgcagc gagcgattga atgtgcacga gcccagattc
     1561 accgtgatcg cccccaccac gccgatggca tagagattgg caagcgattc aatgtcggcc
     1621 ccggtgacca gaacgatcag gatcggcagc aatgtcgcca tcgtccacgg ccaccacggc
     1681 acgccataag cgttcaaccg ggtaaagaaa cgcggcatct cgccgtcgcg tgccagcatg
     1741 tagatgaggc caatctgggc tgcgatcgcc gtgttcacgg cgctcaacag cagcaggccc
     1801 accacaagac cgacaaccgc gccaatcgcc ttcccgactc ccgcgttgaa catcagtgtc
     1861 ccgtactcct gcccgagaaa ccgcatcatg tagtcgcgat gtgcgacggg gtcgggcgca
     1921 tgcgcgccgg acaacgagag cattgcccag cccagcagag ccgtccccag cacaacctcg
     1981 gccgcgacgg gcaggatggc tttgcgggcc gtcttggaca cgcacggctt gtccatcgtg
     2041 gcgcccggat cgagcttcat cacccccgtc aggttggcga tcgcctcgac gccgctcagc
     2101 gccagaatcg tcgccacaaa gaactgccag ttgcgggcga tcccgccacg gggcggttcg
     2161 aggtgtgcag tgctgagccg gggcaggctc agcagaatca gcgcgaccac caccacaacc
     2221 ataggaacca tcagccccac cgcgaggctg ccggtatgtt tcggaccgaa gaagttgatc
     2281 ccgccgataa tcaggatgac caggaccgtc gccgccgcga cctgccccgc cgagaccccg
     2341 aaatagtgca tcgccgagtt gcaactcaac gcggcggtga ccagaaaatt ggcgaccagc
     2401 agcagggcgc caatcaccgc cagcagtcgg ctctgatccc gcgcggctga atacacgccg
     2461 cccccgtccg ggaaatgccg gcagacaagg atgtagttgt atcccaccag cgcagtcaac
     2521 acgcacaccg cgccgatgat gggcagcgag gcatggccgg cggcagcgaa agccaggccg
     2581 atgacgtagg ccttgctcgt gccccagtcc ccgtaaagca gggccgctgc gcgcttccaa
     2641 tcgagatttc gcgggcggtg gatggcttga ctgtccatga attgaatggg gccacgacga
     2701 gactggtgcc agaggaggga attgaacccc cgaccaaggg cttatgagtc ccctgctcta
     2761 ccgctgagct actctggca
//
LOCUS       DAOQRO010000400         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_42884_length_2774_cov_4.745127, whole genome shotgun sequence.
ACCESSION   DAOQRO010000400 DAOQRO010000000
VERSION     DAOQRO010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42884_length_2774_cov_4.745127"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKK20_12315"
     CDS             <1..585
                     /locus_tag="PKK20_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV00714.1"
                     /translation="HGALVIVNDSHAVGFMGRTGRGTHEHHQVMGRVDLLTGTLGKAL
                     GGASGGYVSGRRVLVELLRQRSRPYLFSNSVAPPIVAASIKVLEMLEGSTALRDRLEC
                     NTRFFRDGLSRAGLTVLPGTHPITPIMLGDAALAGRFADAMLRKGVYVIGFSYPVVPK
                     GKARIRTQVSAAHTEDDLAFALKAFAEVKAELSL"
     gene            634..1914
                     /gene="thiH"
                     /locus_tag="PKK20_12320"
     CDS             634..1914
                     /gene="thiH"
                     /locus_tag="PKK20_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNV00715.1"
                     /translation="MSFVSALDTLPLTSLVDRARTAPESAVRSSLARTTLSLADFANL
                     ISPAASGHLETMGRRSHALTVQRFGQTIRLFAPLYLSSECVNNCVYCGFSRKNTIRRV
                     TLSVDEVVREARALKAEGFRNILLVAGEHPKFVSNRYVRDCVAAVHAEVPSVSLEIGP
                     LDVDAYRHLVEAGADGLVVYQETYDRALYASLHPSGPKRNFDRRLETPERAYAAGFRR
                     IGIGALYGLADWRADALSLAAHALYLLRHCWKAYLTISLPRLRPCAGAFQPLTHLGDR
                     DLAQLVCAFRLLLPDVGLVLSTREPARLRDGLIPLGITLISAGSHTEPGGYSGAGREK
                     THVTRGGRMFESVHDPSQWTAAPPGITDADVQFAIADQRSASEVSARLRDLGYEPVWK
                     DWDSALTPSRNGEGEGGSKEERGKGETCGAKEMA"
     gene            1911..2120
                     /gene="thiS"
                     /locus_tag="PKK20_12325"
     CDS             1911..2120
                     /gene="thiS"
                     /locus_tag="PKK20_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNV00716.1"
                     /translation="MNSGVVSANGRPVTVALPCTIEQFLIARDLPPRTVVVEQNGEAV
                     APSEFPHRPVCDGDRLEIIRIVAGG"
     gene            complement(2217..>2774)
                     /locus_tag="PKK20_12330"
     CDS             complement(2217..>2774)
                     /locus_tag="PKK20_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00717.1"
                     /translation="VSCFGSGTIRKELIRCYDSPFAVETTHIKFRASDLSARIYRSLF
                     DVARQYRESFDVYGSKKTVEWPLIEHEPLIVHTAKLPEPKIPKPVRCPDFAGRLPKTI
                     ARYTTRGVYDSRKKTHLSFTQGAGHGGSHPHLAHEFATALAENRDPFPNARQSANWTC
                     VGLCAHQSALRGGAIVKLPAFTLGS"
BASE COUNT          446 a          904 c          921 g          503 t
ORIGIN      
        1 cacggcgccc tggtgattgt caacgactcg cacgcggtcg ggttcatggg acgcaccggg
       61 cgcggcaccc acgagcacca ccaggtgatg ggccgcgtcg atctcctcac cggcactctg
      121 ggcaaggccc tcggcggcgc cagcggcggc tatgtgagcg gacgccgcgt tctggtggaa
      181 ctgttgcgcc agcgctcgcg cccatacctc ttctcgaact ccgtcgcccc gcccatcgtg
      241 gccgcgtcca tcaaggtgct cgagatgctc gagggctcga ccgccctgcg cgaccggctc
      301 gaatgcaaca cccgcttctt ccgcgacgga ctgtcccggg cggggctcac ggtgcttccg
      361 ggcacccatc ccatcacgcc catcatgctt ggcgatgccg cgttggccgg gcgtttcgcg
      421 gacgcgatgc tgcggaaggg cgtctatgta atcggctttt cctacccggt cgtccccaag
      481 ggcaaggccc gcatccgcac gcaggtctcg gcggcgcaca ccgaggacga cctggcgttt
      541 gccctgaagg ccttcgccga ggtcaaggcc gagctgagcc tctgatccca atctgcgcct
      601 cgacttcgcc cccaactctg atagggtgag agcgtgagtt tcgtttccgc attggacacg
      661 ctgcctctga cgagcctggt cgaccgcgcg cgcacagcgc cggagagcgc ggtgcgctcg
      721 agcctcgcgc ggaccacgtt gtccctggcc gacttcgcca atctcatttc gccggctgcg
      781 agcgggcatt tggagactat ggggcgccgg tcccatgcgc tgaccgtcca gcgttttggc
      841 cagacgatcc gtctgttcgc ccctctgtac ctgtcgagcg agtgcgtcaa taactgcgtc
      901 tactgcggct tctcgcgcaa gaacaccatt cgtcgcgtga cgctttcggt cgatgaggtc
      961 gtccgcgagg cgcgcgcctt gaaagcggag gggttcagaa acattcttct ggttgcgggc
     1021 gagcatccca agtttgtttc gaaccgctac gtgcgcgact gcgtggccgc cgtgcacgcc
     1081 gaagtgccca gcgtctcact cgagatcggc ccgctcgatg tcgatgccta ccgtcatctg
     1141 gtcgaggctg gggccgatgg tctggtcgtc tatcaggaga cctacgaccg cgctctgtac
     1201 gcttcgctcc atccctcggg cccaaagcgc aatttcgacc gccgcctcga gaccccggag
     1261 cgcgcgtatg ccgccggatt tcgccgcatc gggattggcg ccctctacgg gctggcggat
     1321 tggcgggcgg acgcgttgag tctggccgcg catgcgctct acctgctgcg ccattgttgg
     1381 aaagcctatc tgaccatctc gctgccgcgg ttgcgaccat gcgccggggc cttccagccc
     1441 ttgacccatc tgggtgacag agacctggca caactggtct gcgccttccg cctgctcctg
     1501 cccgacgtcg gtctggtgct gtccacgcgc gaaccggccc ggctgcgcga cggcctgatc
     1561 ccgcttggca tcacgctgat cagcgcgggc agtcataccg aaccgggcgg gtactccgga
     1621 gccggccgcg agaagacgca tgtgacgcga ggagggcgga tgttcgagtc ggttcatgat
     1681 ccgagtcaat ggaccgcggc gccaccggga atcaccgacg ccgacgtcca attcgcaatc
     1741 gccgatcaac ggtcggcttc ggaggtctcg gctcgactgc gcgacttggg ctatgaaccg
     1801 gtctggaagg actgggattc ggcgctcact ccttcgagga acggggaggg agagggagga
     1861 agcaaagagg aaagggggaa gggcgaaaca tgcggggcaa aggagatggc atgaacagcg
     1921 gtgttgtgtc tgcaaacggc cggcccgtga ccgtggcgct gccctgcacg atcgaacaat
     1981 tcctgatcgc ccgtgacctc ccgccgcgca ccgtcgtggt cgagcagaac ggcgaagctg
     2041 tcgctccgtc cgaattcccg caccgccccg tgtgcgacgg ggaccgtctc gagatcattc
     2101 ggatcgtggc gggaggatga gtcggcccac cggagacgcc caggccctga tacctgggca
     2161 ccctggtcga cgcggaatgt ccgaacctgg tctgggcgcg agaccgactg ggccgttcac
     2221 gatccaagcg tgaacgccgg cagcttgacg atcgcccctc cgcgcagggc tgactgatgg
     2281 gcgcagagac cgacgcaggt ccagttggcc gactgtcggg cgttggggaa cgggtcccgg
     2341 ttctcggcga gcgcggtggc gaactcgtgt gccaagtggg ggtgactgcc gccgtgtccg
     2401 gccccctgag tgaagctcag gtgcgtcttc ttgcgcgagt cgtagacgcc tcgggtcgtg
     2461 tagcgggcaa tcgttttcgg caggcggccg gcgaaatccg ggcagcggac cggcttggga
     2521 atcttgggtt cggggagctt ggccgtgtgg acgatcagcg gctcatgctc gatcagaggc
     2581 cactcgacag tcttcttgct gccgtagaca tcgaagctct cgcggtattg ccgggcaaca
     2641 tcgaacaacg accggtagat gcgcgcgctc aagtccgatg cgcggaactt gatatgcgtc
     2701 gtctcgaccg cgaacggcga gtcgtagcac cggatcaact ccttgcggat ggtgcccgat
     2761 ccgaaacagg atac
//
LOCUS       DAOQRO010000401         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_43283_length_2757_cov_5.180607, whole genome shotgun sequence.
ACCESSION   DAOQRO010000401 DAOQRO010000000
VERSION     DAOQRO010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43283_length_2757_cov_5.180607"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2757)
                     /locus_tag="PKK20_12335"
     CDS             complement(<1..>2757)
                     /locus_tag="PKK20_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00718.1"
                     /translation="VELDAIRILLEPLPPHEPPPGFDQTDPAVASDASPLPLARGLVL
                     DWTMANGSQKDVTFRCGSTYLVAGVVNLSGTTTIEGGAVVKYTNNGLARLNVQGDLVC
                     ATAPYRPAVFTANADDSVGDITPRGAATDYYAAVALQMPADGAAISNVRILRARTALY
                     FPPAINSRPRDRVEHVQVVNCQNGVVASGIRYYNMVLRRTLHLGNGLFAREAGLRGTS
                     KAIVGAYLRGTAEHLTLDGWTYMAQATGESSSSLVVRNSVLANVVNTSPTPRLRLQGS
                     GWNGFYATPNFGNQCIEIHSNPFTRAGAGRYYLGAGSPFLDRGTDKIDPELMKELEER
                     TVWPPLARTAAKPVALDTTLGPTAPRDNDGRPDLGWHYAPLDWCWTGLTVAGTNGPAT
                     LLLTNGVAVGIHDSVGTILLDGARFLSEGSPSRYNRLVRCETVQEQPAPRNETGRTAA
                     LFSAAGTRSISKEVHLRFTEVAVLPGKRALFDNTTFLPLGFLSLRDSRFYGVSLDLSL
                     YPPGAAGPASLGLTNNLFHRSSLSLDQDTELQRPLLVVHLRNNLFEGGRLRLADYSGS
                     DSWGAYDNTFDTLESVASEGVPNAHNGYSGEVALSGSAGGDVTHLALSTRTAGSTRGA
                     SLAGLANPAPLVDAGSRSAADAGLYHHATVDGASKEADSQVDIGYHAVATIYGRPYDS
                     DSDGLPDYVEDANGNGVADDRTDWTEAATEPDATGYTDLQRLELASNILVNDPAQDYA
                     NEQNTQYQPGVVVLENTVVVGYWDSNRGIYGLASLDDPPRLCRMVSYSVSHDGGRTFV
                     DRDVLPLPEGATSGDAGDPVLAVDRVSGVIYYAATSERKAGTFKGIPLWKSVDRGARF
                     VRCPPLAEEIEGSDYPWIAVDDWPGSGQHDVYAVIQGSVAGSRRRQWLTVSRDGDVRA
                     WSRPV"
BASE COUNT          479 a          940 c          871 g          467 t
ORIGIN      
        1 caccggtcgg gaccaagccc tgacatcgcc gtccctggac actgtcagcc actggcgccg
       61 tcgcgacccg gcgacgcttc cctgaatgac tgcgtacacg tcgtgctggc cggagcccgg
      121 ccagtcatcg accgcaatcc acgggtagtc cgacccctcg atctcctcgg ccagaggcgg
      181 gcagcggaca aaacgggcgc cgcgatcgac cgacttccac agagggattc ccttgaacgt
      241 gccggccttc ctttccgaag tggccgcgta gtagatcacc cctgacaccc ggtccacggc
      301 cagcacggga tcgccggcgt ctccgctcgt ggcgccctcg ggcaacggca gcacgtcccg
      361 atccacaaag gtccgaccgc catcgtgaga gacggaatac gagaccatgc ggcacagacg
      421 gggcggatcg tcgaggctgg ccagtccgta aatcccccgg ttgctgtccc aatagccgac
      481 gaccactgtg ttttcgagga ccacgactcc aggctggtat tgggtgttct gttcgttggc
      541 gtaatcctgg gccggatcgt tcacaagaat gttcgacgcc aactcgagcc gctgcagatc
      601 ggtgtagccc gtcgcgtccg gttccgtggc ggcctccgtc cagtctgtcc ggtcatcagc
      661 cacgccgttg ccattggcgt cctcgacgta atccggcaaa ccgtccgaat ccgagtcata
      721 cggacgcccg taaattgtcg caaccgcgtg gtacccgatg tcaacctgag agtcggcttc
      781 cttcgaggcc ccgtccaccg tggcgtgatg gtagagcccc gcgtccgccg cactccggct
      841 gcccgcatcg accagcgggg ccggattggc gagccccgcg agtgacgcgc cgcgggtcga
      901 cccggcggtc cgggtgctga gcgcaagatg tgtgacgtcc cctccggcgg atcccgaaag
      961 ggcaacctcc ccgctgtagc cgttgtgcgc gttgggcacc ccctcggatg cgacgctttc
     1021 caacgtgtca aacgtgttgt cgtatgcccc ccacgaatcc gatcccgagt aatccgccag
     1081 cctcagcctg ccgccctcga acaggttgtt gcgcaaatgg acaaccagca atggacgctg
     1141 caactccgtg tcctgatcca gactcaaact cgaccgatga aacaggttgt tggtcagtcc
     1201 aaggctcgca ggccctgccg caccgggcgg atacagcgac aagtccaggc tcaccccgta
     1261 gaaccgagag tcgcgcagag acagaaaccc cagcgggagg aaagtcgtgt tgtcaaacag
     1321 cgcccgcttc ccgggaagca ccgcaacctc cgtgaaccgg aggtgaacct ctttcgagat
     1381 ggaccgggtg cccgcagccg agaacagggc ggccgtgcgt cccgtctcgt tacggggcgc
     1441 gggctgttcc tggaccgttt cgcaccgcac caggcggttg tagcgcgatg gcgagccctc
     1501 actcaaaaaa cgggctccat cgagcaggat ggtgccgacg ctgtcgtgga tgcccacggc
     1561 aacgccgttg gtgagcagca gcgtcgccgg cccgttggtg cccgccaccg tcagccccgt
     1621 ccagcaccaa tcgagcgggg catagtgcca ccccaggtcc ggccgcccgt cgttgtcccg
     1681 cggcgcggtc gggccgagcg tcgtatcgag tgcgaccggc ttcgcggcgg tccgcgccag
     1741 cgggggccag accgtccgct cttcgagttc cttcatcagt tcggggtcga tcttgtcggt
     1801 ccctcgatcc aggaatgggc tgccggctcc aaggtagtag cggccggcgc cggcccgcgt
     1861 gaacggattg ctatgaatct cgatgcactg gttgccgaag ttcggcgtgg catagaaccc
     1921 attccacccg ctgccctgca gacgcaggcg cggcgttggg ctcgtgttga ccacgttggc
     1981 gaggacggag ttccggacga ccagcgaact cgaggattcg ccggtggcct gggccatgta
     2041 ggtccagccg tccagggtca gatgctcggc tgtgcctcgg agataggcgc cgacaatggc
     2101 tttgctggtc cccctcaggc cagcctcgcg cgcgaacagc ccgttgccga ggtggagggt
     2161 ccgcctcaga accatgttgt aataccggat gcccgacgca acgaccccgt tctggcagtt
     2221 caccacttgc acatgctcga cccggtccct tggtcggctg ttgatggccg gcgggaaata
     2281 cagcgcggtt cgagcgcgaa gaatccgcac gttggaaatg gccgccccat cggccggcat
     2341 ctgaagcgcc accgccgcgt agtaatccgt cgccgcgccc cgaggcgtga tgtcgccgac
     2401 actgtcatcg gcgttggcgg tgaaaacagc cgggcgatac ggcgccgtgg cgcagaccag
     2461 gtcgccttga acattcagcc gcgcgaggcc gttgttggtg tacttgacca ccgcgccccc
     2521 ttcaatggtc gtcgtcccgc tcaggttgac cacgcccgcc accaggtacg tcgagccgca
     2581 ccggaatgtg acgtccttct ggctcccatt ggccatggtc caatccagca ccagaccccg
     2641 cgccaggggt aggggagacg catcggacgc gaccgccgga tcggtctgat cgaagccggg
     2701 gggcggctca tgcggaggca atggctcgag cagaatgcgg atcgcatcca actcgac
//
LOCUS       DAOQRO010000402         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_44955_length_2688_cov_4.917964, whole genome shotgun sequence.
ACCESSION   DAOQRO010000402 DAOQRO010000000
VERSION     DAOQRO010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44955_length_2688_cov_4.917964"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKK20_12340"
     CDS             <1..751
                     /locus_tag="PKK20_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HNV00719.1"
                     /translation="ISGNRKTYALCLIGGVRWDLTLELIAELRRVLDALEVEWRAVIK
                     AVLGDKNLQQRARGVGVADRSLVEELSLIGPVARAAGVPLDCRRDHPYAAYDRVEFEV
                     VTHEGGDVWARIVVRMKEVFESIEIIRQCLDKMEPGPIQLAIADELPVGRYGLSSVEA
                     PRGESHHFVITGENHRPRRWRVRAPTYQNLQAVPAMIKDQQLADMTITLGSIDPCFSC
                     TDRFEIVDLETSATRVWSAEELARLARAGRR"
     gene            762..1679
                     /locus_tag="PKK20_12345"
     CDS             762..1679
                     /locus_tag="PKK20_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNV00720.1"
                     /translation="MELLTILGCVANAGLVLALAPLGEGVLRKVTARLQSRQGPPLLQ
                     PYFDLLKLLGKEDIESGETPRVQRGAGYLALATVLTVACLLPLGFRTPLAGVGDVILL
                     VYLITLAGICTLLAGWGAGSTYALLGSSREMMTMMALEPLFAVSLVAGAVQTGSCQVD
                     RVLDGSVYGTGGVPWSGLILLMTMALAFPAFVQRLPFDVAEAETEIMEGALIEYSGPK
                     LALFKYAQMVKLLVYSALFVALFVPWGSDRGFPLGWVLFWGKVLVMVLLVTAVAASHA
                     RYRIDQALRHFARLLAFAVGALVLACYGL"
     gene            1711..2244
                     /locus_tag="PKK20_12350"
     CDS             1711..2244
                     /locus_tag="PKK20_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV00721.1"
                     /translation="MPMTSLLSKIREIMVGWNAGRVTLPYPAAPAPTPPRFRGRPIFD
                     ATKCIGCAGCANNCPAREILVYDVCQEIRILKYLGRRCTYCGRCADVCPEGAITMSGE
                     FENATPAIGDIEQRLELFMSTCQRCGRCFTEPSPLEQLKLKGYRFDDLEHDRWVFRSQ
                     GYLENSPVVDDLTIELD"
     gene            2257..>2688
                     /locus_tag="PKK20_12355"
     CDS             2257..>2688
                     /locus_tag="PKK20_12355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNV00722.1"
                     /translation="MLKNICQKMLRRSLWVYHANSGGCNGCDIEVLNVLTPYYDAERF
                     GIKLVGSPRHADVMLCQGPAMRSTACALRRAYEAMPAPKLVFAIGSCACGGGLWHDSY
                     SVLGPVEKLLPVDFYIPGCPPRPEAILFGVAAAAGLVDKKVA"
BASE COUNT          416 a          759 c          974 g          539 t
ORIGIN      
        1 gatcagcggc aaccggaaga cctacgccct ctgcctcatc ggcggggtgc ggtgggatct
       61 cacccttgaa ctgattgcgg aattgcgtcg ggtcctcgat gcgctcgaag tcgaatggcg
      121 cgccgtgatc aaggcggtgc tcggggacaa gaacctgcag cagcgcgcgc ggggtgtggg
      181 cgtggcggac cggagcctgg tcgaggaact ctcgttgatc gggccggtgg cgcgggcggc
      241 gggcgtgcct ctggattgcc ggcgggatca tccctacgcg gcctacgacc gcgtcgagtt
      301 cgaggttgtg acgcacgagg gcggggatgt ctgggcgcgc attgtggtgc ggatgaagga
      361 ggtgttcgag tccatcgaga tcattcgcca gtgcctcgac aagatggagc cgggtccgat
      421 ccagttggcg attgcggacg aactgcccgt ggggcgctac gggttgtctt cggtcgaggc
      481 gccgcgggga gagagccatc acttcgtgat cacgggcgag aaccatcgtc cgcgtcggtg
      541 gcgtgtgcgg gcgccgacct accagaacct gcaggctgtg ccggccatga ttaaggacca
      601 gcaattggct gacatgacga tcaccctggg aagcatcgat ccctgtttct cctgcacgga
      661 ccgtttcgag attgtggatc tggagacctc tgccacgcgc gtctggtcgg cggaggagtt
      721 ggcgcggctg gcgcgcgcgg gacgccgttg aaagagaggc catggagtta ctgacgatac
      781 tcggatgcgt ggcgaacgcg ggtctggttc tggcgctggc gccgctgggc gaaggcgtgt
      841 tgcgcaaggt gaccgcccgg cttcaatcgc gccaggggcc cccgctgctc cagccttact
      901 tcgatctgct caagcttttg ggcaaggagg acatcgaatc gggcgagacc ccgcgcgtcc
      961 agcggggggc gggttacctg gcgctggcga cggtgctgac agtggcgtgt ctgctgcctc
     1021 tgggttttcg gacgccgctg gcgggggtgg gggacgtgat tctcctggtt tacctgatca
     1081 cgctggcggg catttgcaca ttgctggccg ggtggggggc aggatcgacg tacgcgcttc
     1141 tgggctcgag ccgggagatg atgacgatga tggccctcga accgctcttt gccgtgagcc
     1201 tggtggccgg agcggttcag accgggtcct gccaggtgga ccgggtgctt gacggatcgg
     1261 tgtacggcac gggcggcgtc ccgtggtcgg gactgatcct tctgatgacg atggcgctgg
     1321 cgtttccggc ctttgtgcag cggctgccct ttgacgttgc cgaggcggaa acggagataa
     1381 tggagggggc cttgatcgag tactcgggac ccaagctggc gttgttcaag tatgcgcaga
     1441 tggtcaagct gctggtttac agcgcgctgt ttgtggcgct ctttgtgcct tggggaagcg
     1501 acaggggatt cccattgggc tgggtgctgt tttgggggaa agtgttggtg atggtgctgc
     1561 tggtgactgc cgtggcggcc agtcacgcgc ggtaccggat cgaccaggcg ctgcggcact
     1621 tcgcgcgtct gctggcgttt gcggtggggg cgctggtcct cgcctgttac ggtctatgaa
     1681 accgcggccg accacgagcg ggaaaggaac atgccgatga cgtcgttgct cagcaagatc
     1741 cgggaaatca tggtcggttg gaacgccggg cgggtgaccc tcccgtatcc ggccgcgccc
     1801 gcgccgactc cgcctcggtt ccggggccgt ccaatcttcg atgccaccaa gtgcattggc
     1861 tgtgcgggct gtgccaacaa ttgcccggcg cgggagattc tggtctatga cgtctgccag
     1921 gagatccgca ttctcaagta tctggggcgt cgttgcacgt attgcgggcg ctgcgcggat
     1981 gtgtgtccgg agggcgcgat cacgatgagc ggcgagttcg agaatgcgac tcccgcgatc
     2041 ggcgacatcg agcagcggct cgagctgttc atgagcacgt gtcagcgctg cgggcgctgc
     2101 ttcaccgagc cgagccccct cgaacagttg aagctgaagg gctaccggtt cgacgatctc
     2161 gagcacgatc gctgggtgtt ccggtcgcag gggtatctcg agaattcgcc cgtcgtggac
     2221 gatctgacga tcgaactgga ttgagtcagg aaggctatgc tcaagaatat ctgccagaag
     2281 atgctgcgcc gctccctgtg ggtctatcac gccaacagcg gcggctgcaa cggatgcgac
     2341 atcgaggtcc tgaatgttct caccccgtat tatgacgccg agcgtttcgg aatcaaactg
     2401 gtgggatcgc cccgtcacgc cgatgtgatg ctgtgccagg ggccggcaat gcgttcgacg
     2461 gcgtgcgcct tgcgccgcgc ctacgaggcc atgccggcgc caaagttggt ttttgccatc
     2521 ggctcatgcg cctgcggcgg tggcctgtgg cacgacagtt acagcgtcct ggggcccgtc
     2581 gagaaactgt tgcccgtcga tttctacatc cccggatgcc ccccccggcc cgaggcgatc
     2641 ctcttcggcg tcgcggccgc ggcggggttg gtggataaga aagtcgcc
//
LOCUS       DAOQRO010000403         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_45635_length_2662_cov_4.579210, whole genome shotgun sequence.
ACCESSION   DAOQRO010000403 DAOQRO010000000
VERSION     DAOQRO010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45635_length_2662_cov_4.579210"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKK20_12360"
     CDS             complement(<1..338)
                     /locus_tag="PKK20_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HNV00723.1"
                     /translation="MNQEIAALLDLSHELGREERQLAILSEGNASARLDGGQFALKAR
                     GARMASLVESDLTVCRGAPMIELLDRKTLPDATIEKALLDARVDFAAQMPGLDSIFHA
                     WLLTLENVRFV"
     gene            457..1644
                     /locus_tag="PKK20_12365"
     CDS             457..1644
                     /locus_tag="PKK20_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00724.1"
                     /translation="MKARSHSTDDASRTAGDRSRTHTPDRSVPARRSSSVQSVLHAVA
                     MPILCGCLGACTSGAEPGQTGRDQSLPQIRVTSVRRVFHNGEHNAFTDLVRFKGRLFL
                     TFRSCPDGHSVHPTASILILASDDDGRQWKQVHRFRVAHRDTRDPHFLVFQDKLFVYT
                     GTWYSGPTTLPPKDWDLNLHLGYAAWSQDGVQWHSPILLEGTFGHYIWRAGAFGGKAY
                     LCGRRKIGFAVGPKGEGREVESLMLESDDGLIWRKRAVFQEIAGDETAFLFEPDGRVL
                     GIGRRHGTAQLLRSAPPYKRWDRLDLDRSIGGPMLVKWGDRCLVGGRDSTPDRGPKTA
                     LWWLVGDRLHPCAELPSGGDNSYSGFVELDGGRALLSYYSSHELDASGKPITAIYLAE
                     IAL"
     gene            1815..>2662
                     /locus_tag="PKK20_12370"
     CDS             1815..>2662
                     /locus_tag="PKK20_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNV00725.1"
                     /translation="MQNTFHQPLDSDEAGRKSRQVVVSVRVLLRLSLLLLLSGCGERP
                     RHYQGSVSCKECHARFYELWEPSHHGRALQPWSASLAALVPPQHEPIEVDGHMYRADS
                     TPGKGWVEDETGLQYLIAYALGGKNYYNFLTRLDDGRLQVLPIFYDVREKRWRNTTLG
                     MVRHFENGLEDRPVSWRDPMLTFNAACFSCHVSQLESNYDPQTDSYATTWNEPGISCE
                     SCHGPASEHVRVCRAAPANRPPGDLEIIAWHDLSIEQQNDSCSGCHAKATPLTARFET
                     GDRFWD"
BASE COUNT          491 a          930 c          773 g          468 t
ORIGIN      
        1 acgaaacgca cattctcgag ggtcaacagc caggcatgaa aaatcgaatc caggccgggc
       61 atctgcgcgg cgaaatcgac tcgcgcgtcg agcagggcct tctcgatcgt ggcgtcgggc
      121 agggtcttgc ggtccagcaa ctcgatcatc ggcgcccccc ggcacacggt caggtcactt
      181 tcgacgagtg acgccatccg agcgcccctc gccttgaggg cgaattgccc gccatcgagc
      241 cgggcggatg cgttgccttc gctcaggatg gccaattggc gctcttcgcg cccgagttca
      301 tgcgagaggt ccagcaaggc ggcaatctcc tggttcatgc gccgcccatt gaacatcgaa
      361 tgccctcccg cgccaagaca caaacaccct cgctgcaaat gcgccccctg cgaatgagct
      421 tgcccgccag gaatcctcga gccgaatctc gcccctgtga aagcacgttc ccactcgacc
      481 gacgacgctt cgagaaccgc tggcgaccgc tcccgcacac acacacccga ccgatccgtt
      541 ccggccagac gatcatcctc ggttcagtcc gtcctccacg ccgtggcgat gccgattctc
      601 tgcggctgtc tcggcgcctg cacgtccggc gccgagcccg ggcagaccgg gcgcgaccaa
      661 tccctgccgc agattcgggt gacatccgtg cgacgcgtgt tccataacgg tgagcacaac
      721 gcgttcaccg atctggtccg attcaaaggc cggctgtttc tgacctttcg aagttgtccg
      781 gacggacact cggtgcatcc gacggcctcc attctgatcc tggccagcga cgacgacggg
      841 cggcaatgga agcaggttca ccggttccgc gtcgcccaca gggacacccg cgatccgcat
      901 tttctggtct tccaggacaa gctctttgtc tacacgggaa cctggtacag cggcccaacc
      961 acactgccgc ccaaggattg ggacttgaac ctgcatctgg gctacgcggc ctggtcccaa
     1021 gatggagtcc aatggcacag ccccatcctc cttgaaggga ccttcggaca ctacatctgg
     1081 cgcgcggggg cgtttggcgg caaggcctac ctgtgtggcc ggcgcaagat cggctttgcc
     1141 gtcggtccca agggagaagg ccgtgaggtc gaatccctga tgctcgaaag cgacgatggc
     1201 ctgatctggc gcaaacgcgc cgtgtttcag gagatcgccg gggacgaaac agcgttcctc
     1261 ttcgagccag acggccgggt gctgggaatc gggcgccgtc acggcacggc ccagcttctt
     1321 cggtcagccc ccccctacaa gcgatgggac cggctggacc tggaccgctc gattgggggc
     1381 ccgatgctgg tcaagtgggg cgaccgctgt ctggttggcg gacgcgactc gacaccggat
     1441 cgcggcccga aaacggccct gtggtggctg gtcggcgatc gcctccaccc ctgcgccgag
     1501 ttgcccagcg gcggcgacaa ctcctactcc ggctttgtcg aattggacgg cggccgcgct
     1561 ttgctctcct actactcgag ccacgaactt gatgcctcgg gcaaaccaat caccgccatc
     1621 tatctcgccg agatcgcgct ctaatctaat agagaatcct cgaccccatc aacccggcat
     1681 acatacttga cttctctgta cacagcctca gaagctccgc cgcgtgaggg cacgcggccc
     1741 acaggcttgt aggccgggtc gcctgcctgc cggcaggcag gcctgacccc gcgctgcatc
     1801 gtttatgttg ctctgtgcag aacacattcc accagcccct ggattctgac gaggccggtc
     1861 gcaagagccg ccaggtcgtc gtttccgtcc gagtgttgct caggctcagc ctgctgctgc
     1921 tgctgtccgg ttgtggggag cgtccgcggc attaccaggg ctcggtgagc tgcaaggagt
     1981 gccacgccag attctacgaa ctctgggaac cctctcatca cggacgtgcc ctccaaccat
     2041 ggtccgcctc tctcgccgcc ctggtgccgc cccagcacga gccgatcgaa gtcgacgggc
     2101 acatgtatcg cgccgactcg acccccggga agggatgggt cgaagacgaa accgggctgc
     2161 agtacctgat cgcctatgcc ctgggcggca agaactacta taacttcctg acccggctcg
     2221 acgatgggcg cctgcaggtg ctgccaatct tctatgacgt gcgcgaaaag cgctggcgca
     2281 acacaacgct cggcatggtc cgccatttcg agaacggtct cgaggatcgg ccggtgtcgt
     2341 ggcgggaccc gatgctcacc ttcaacgccg cctgcttcag ttgccacgtc agccaactcg
     2401 aatcgaatta cgacccccaa accgacagct acgccaccac ctggaacgag ccgggcatca
     2461 gttgcgagtc ctgccacggc cccgcctccg aacacgtccg cgtctgccga gccgccccgg
     2521 ccaaccgtcc gcctggcgac ctcgagatca tcgcctggca tgacctgagc atcgagcagc
     2581 aaaacgactc ctgctccgga tgccacgcca aagccacccc actcaccgcc cggttcgaaa
     2641 caggcgaccg cttctgggat ca
//
LOCUS       DAOQRO010000404         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_46077_length_2645_cov_3.759073, whole genome shotgun sequence.
ACCESSION   DAOQRO010000404 DAOQRO010000000
VERSION     DAOQRO010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46077_length_2645_cov_3.759073"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2645
                     /locus_tag="PKK20_12375"
     CDS             <1..>2645
                     /locus_tag="PKK20_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNV00726.1"
                     /translation="KAADTIELELPMEVQRIVSDERVFANRGFTALRYGPLIYCVERA
                     DQPVITGAIGPQPLATEWRGDFLHGVKVIKGTWDDGSPLLAIPYYARDNRPDTASSRR
                     RAESQVWIKTAPTDQQTATIGNNALSDETTAASSQPATASKQAEATSAPPWSGSELLA
                     VPPISIETITGTLPRLPRNIAGQFRDGTTGTVVRVIWPSPTNNQSVLEPGTFTVTGRV
                     AGTTIEPKAVVTVKPASSDSPAPTRGVEAFPLDRVSLDRDEQQRETPFIRHRNKFIQG
                     LAQTDPDRFLYMFRDAFGQPQPETAQPLGVWDSQTSRLRGHASGHYLSAIAQACASTG
                     YDPALQTNFLSKLTYLIDTLYDLSQKSGRPTREAGPCNADPTAVPPGPGVTNYNSNLT
                     SEGIRTDYWNWGLGFISAYPPDQFIMLEQGASYGDGNHQVWAPYYTLHKILAGCLDCY
                     EVTRNQKALEIARGMGLWVQQRLQAVPPAIRIRMWNRYIAGEYGGMNEVMARLFRLTG
                     DVRFLDCARLFDNSNFFFGDADHTHGLARNVDTLRGRHANQHIPQITGALEIYRNTGE
                     RPYLSVAEHFWGICTGGYMYSIGGVAGACNPNNAECFTAEPDSLFANGFNRGGQNETC
                     ATYNLLKLSRQLFMYDPDARYMDYYEQALYNHILASVAAEDSANTYHVPLNPAARKQF
                     GNGGMDGFTCCNGTALESATKLQDSIYFHGADHQTLFVNLFIPSTLRWTERNLTLTQR
                     TSYPFEETTRLVLAGNGAFDLKVRVPRWATRGFHVCINGQSKQVNAIPGSYLNLGRDW
                     KDKDTIELHAPFPFHLSRVMDQPNIASLFYGPILLAAEESRPRSDWRPVVLDAADIGK
                     SITGNPRTLRFDIGGVMFRPFFEMY"
BASE COUNT          538 a          998 c          680 g          429 t
ORIGIN      
        1 gaaagccgcc gacaccatcg aactcgaact gccgatggag gtgcagcgta tcgtgtcgga
       61 cgaacgggtc ttcgccaatc gcggtttcac agctctgcgc tacggccccc tcatctactg
      121 cgtcgagcgc gcggatcaac cggtcatcac cggcgcaatc ggcccccaac ccctcgccac
      181 cgagtggcgc ggcgatttcc tgcacggggt caaggtcatc aaaggcacct gggacgacgg
      241 ctcgcccctg ctggccatcc cctattatgc ccgcgacaat cggcccgaca cagcctcctc
      301 ccgacgccgc gctgaatcac aagtctggat caagaccgcc ccgacagacc agcagaccgc
      361 aacgatcggc aacaatgcgc tgtcggacga gacgacagcc gcgtccagcc agcccgccac
      421 cgcctccaaa caggctgagg caacttctgc gccgccgtgg tccggcagcg aattgcttgc
      481 cgttccgccc atctcgatcg agaccatcac aggcaccctg ccccggctgc cccggaacat
      541 cgccggtcaa ttcagggacg gaaccacggg aaccgtggtc cgcgtcatct ggccgtctcc
      601 caccaacaat cagtccgtcc tcgaaccggg cacgttcacc gtcaccggac gcgtcgccgg
      661 gaccacgatc gagcccaagg ccgtcgtcac ggtgaaaccc gcctcgagtg attcgcccgc
      721 tccgacccgc ggggtcgaag cgtttccgct ggaccgggtg agcctcgacc gcgacgaaca
      781 gcagcgcgag accccgttca tccgacaccg caacaagttc atccaggggc tggcccagac
      841 cgatcctgac cggttcctct acatgttccg cgacgccttt ggacaacccc aacccgagac
      901 tgcccaaccg ctgggcgttt gggattccca aacaagccgc ttgcgcggcc atgccagcgg
      961 ccattacctg tccgccatcg ctcaggcctg tgccagcacc ggctacgatc cggcgctgca
     1021 aaccaatttc ttgtcgaagc tgacctatct catcgacacc ctgtacgacc tgtctcaaaa
     1081 gtcaggccgc ccgacccgag aagccggccc ctgcaacgcc gatcccaccg ccgtgccgcc
     1141 cgggcccgga gtcacaaact acaactccaa cctgaccagc gaaggcatcc gcacggacta
     1201 ctggaattgg ggactgggct tcatcagcgc ctatccgccc gaccagttca tcatgctcga
     1261 acagggggcg agctacgggg acggcaacca ccaggtctgg gccccctact acacgctcca
     1321 caaaatcctc gccggatgcc tcgactgcta cgaagtgact cgaaaccaaa aggccctcga
     1381 gatcgcacga ggcatgggcc tctgggtgca gcagcgcctc caagccgtcc cgcccgccat
     1441 tcgcattcgc atgtggaatc gctacatcgc cggcgagtac ggcgggatga acgaagtcat
     1501 ggcccgcctc ttccgcctga ccggagacgt gcgcttcctt gactgcgccc gccttttcga
     1561 caacagcaac ttcttcttcg gcgacgccga tcacacccat gggctggccc ggaacgtcga
     1621 cacgctgcgc ggacgccacg ccaaccaaca catcccccaa atcaccggcg cgctcgaaat
     1681 ctatcgcaac acgggcgagc gtccttacct cagtgtcgcg gaacatttct ggggtatctg
     1741 caccggcggt tacatgtaca gcatcggcgg cgtcgccggc gcctgcaacc cgaacaacgc
     1801 cgagtgcttt accgccgaac ccgactcgct cttcgccaat ggattcaacc gcggcggcca
     1861 gaacgaaacc tgcgccacgt acaacctgct caagctgagt cgacagctct tcatgtacga
     1921 ccctgacgcc cgttacatgg attactacga gcaggctctc tacaaccata tcctcgcctc
     1981 cgtcgccgcc gaagactccg ccaacacgta ccacgtcccc ctcaacccgg cagcacgcaa
     2041 gcagttcggc aacggcggca tggacggctt cacctgctgc aacggcaccg ccctcgaaag
     2101 cgcgaccaaa ctccaggatt cgatctattt ccacggcgcc gatcaccaga cgcttttcgt
     2161 caatttgttc atcccctcga ccctccgatg gacggagcgc aatctcaccc tcactcagcg
     2221 caccagctac cccttcgagg aaaccacccg gctcgtcctc gcaggcaacg gcgccttcga
     2281 tctcaaggtc cgcgtgccgc gctgggcaac ccgcggattt catgtctgca tcaacggcca
     2341 gtccaaacag gtcaacgcca tcccgggcag ctacctgaat ctgggccgtg actggaagga
     2401 caaggacacc atcgaactgc acgcgccgtt cccattccac ctcagccgtg tcatggatca
     2461 acccaacatc gccagcctct tctacggccc catcctgctc gcagccgagg aatccagacc
     2521 ccgctccgac tggcggcccg tcgtcctcga cgccgccgac atcggcaaat ccatcaccgg
     2581 caatccgcgg acgctccggt tcgacatcgg cggcgttatg ttccgtccgt tcttcgaaat
     2641 gtacg
//
LOCUS       DAOQRO010000405         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_46326_length_2636_cov_4.977528, whole genome shotgun sequence.
ACCESSION   DAOQRO010000405 DAOQRO010000000
VERSION     DAOQRO010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46326_length_2636_cov_4.977528"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1011
                     /gene="metF"
                     /locus_tag="PKK20_12380"
     CDS             121..1011
                     /gene="metF"
                     /locus_tag="PKK20_12380"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNV00727.1"
                     /translation="MRFIHDIYSDARQAGRPVFSFEFFTPKTESGDRAIFERTLPALC
                     RLAPSYCSVTYGAGGSTRAKTLQMVERIQLEFGVPAMAHLTCVGSTRDQIVALLDEAR
                     SRGISNILALRGDPPEETGRFVRSDGGFEFAHELVTLTRDLGGFSIGVAGFPEGHLEC
                     REGKEVDWRRLAAKIGCGARFVITQLFFDNAFFYEFRDYLVGTLGITVPIVPGVIPIL
                     SSSQVERFVSLCGATIPSALAGRLNALGDNDADVTEFGIEFATRQCADLLRQGVPGIH
                     FYTLNRTEPTTRIVQNLRGG"
     gene            1052..1948
                     /locus_tag="PKK20_12385"
     CDS             1052..1948
                     /locus_tag="PKK20_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNV00728.1"
                     /translation="MYYLTGISDEAGASIDAQIKATCALGWRTLEARTVEVEGFPGAN
                     LHEIPERAFELAVEKLESAGIGVCCFGSTIMNWAKTVQTPFAVTLAETRRAIPRMQRL
                     GTRFVRIMSFKPADDADTIPAVVFERVREVTRMFLDAGLQPVHENCMNHGGMSARHAL
                     ELLENVPGLKWVFDTANPIFNPDRSRPKPWSKQDPWYFWTQVREATMHIHIKDATWNP
                     EKNDADYRWPGEGDGRVRDIVKDALSRGYDGGISIEPHMAVVFHDSNTRASAEAQYAN
                     YIEYGRRLAGLIGEVERELQLA"
     gene            complement(1961..>2636)
                     /locus_tag="PKK20_12390"
     CDS             complement(1961..>2636)
                     /locus_tag="PKK20_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNV00729.1"
                     /translation="DAAIEAKLLQRMLTHAVARSFNRITVDGDMSTNDTVLVLANGLA
                     GHQQLRWRPAAKPSREIGLFQAGLDHVCLELARMIVRDGEGVTRFVTLRLSGARTCAE
                     ADAAVRAVANSALVKASWFGGDPNWGRIIDALGYSPARIVEERVDIGYSAPGAPTVVW
                     SLKKGRPTTTSFKQLCAAVAPREFDLHIRLNLGPASAILYAADLTEEYVDFNKGDVSD
                     PTKLGG"
BASE COUNT          481 a          820 c          839 g          496 t
ORIGIN      
        1 gtccagcaaa atatgctttc cctgaaatca cccaaccaat ttgtcatcca cccccgctgg
       61 cgcacgagtg cgcgcgcggc ttgcgcttcg attgtgcatg gcgcatgata gccatccttg
      121 atgcggttca ttcacgacat ctacagcgac gctcggcaag cagggcggcc ggtgttctca
      181 ttcgagtttt tcacgccgaa gacagagagc ggtgatcggg cgatcttcga gcggacgctg
      241 cccgccctct gtcggttggc gccgtcctac tgctcggtca cctatggcgc cggaggcagc
      301 acgcgcgcga agaccctgca gatggtcgag cgtattcaac tcgagttcgg ggtgcccgcg
      361 atggcgcatc tcacctgcgt tgggtcgacc cgagaccaga tcgtcgctct gctcgacgag
      421 gcgcgcagca ggggaatcag caacatcttg gccctgcgcg gggatccgcc cgaggagacc
      481 ggacgctttg tcagatccga cggcggattc gaattcgcgc acgagctggt gacgttgacc
      541 cgtgatcttg gaggtttctc gataggagtt gcgggtttcc ccgagggcca tctcgagtgc
      601 cgggagggca aggaggttga ttggcgccgc ctggcggcga agatcgggtg cggcgcccgg
      661 ttcgtcatca cgcagttgtt cttcgacaac gccttctttt acgaattccg cgattacctc
      721 gtcggcacgc tgggcatcac ggtgccaatt gtgcccggcg tcatcccgat cctcagttca
      781 agccaggtcg agagattcgt ctctttgtgc ggagcgacca tcccgtccgc cctcgctggg
      841 cggttgaacg ccctgggcga caacgacgcg gacgtgacgg aattcgggat cgagttcgct
      901 acgcgacagt gcgccgacct cttgcgccag ggtgtgccgg ggattcattt ctacacgctg
      961 aaccggacgg agcccacaac gcggattgtc caaaacctca ggggcggctg agcgcgtgcc
     1021 tcgactgccc cggcaaccag tgttacggcc tatgtactac ctgactggca ttagcgacga
     1081 agcgggagca tccatcgatg ctcagatcaa ggcgacctgt gcactcggat ggcgtacact
     1141 cgaagcccgg acggtcgagg tcgaaggttt ccccggcgcc aatctccatg agatccccga
     1201 gcgcgcattc gaactggctg tcgagaaact cgaatcggcg ggcatcggcg tctgctgctt
     1261 cggatcgacg atcatgaact gggcaaagac cgtgcagacc ccctttgcgg tgaccctcgc
     1321 cgaaacgcga cgcgccatcc cgcgaatgca gcggctcggc acacgattcg ttcgcattat
     1381 gagtttcaag ccggccgatg acgccgacac gattcccgcc gtggtcttcg agcgggtcag
     1441 ggaggtgacc cggatgttcc tcgatgccgg cttgcagccc gtgcacgaga actgcatgaa
     1501 ccacggcggc atgagcgcgc gccatgcgct cgaattgctc gagaacgtgc ccggactcaa
     1561 gtgggtgttc gacactgcca accccatctt caatcccgac cgttcccggc ccaagccatg
     1621 gagcaagcag gatccttggt atttctggac tcaagtccgc gaggcgacga tgcatataca
     1681 tatcaaggac gccacatgga acccggagaa gaacgacgcc gattaccgat ggccgggcga
     1741 gggcgacgga cgggtgcgcg acattgtcaa agacgcgctg agccgcggat acgacggcgg
     1801 catctcgatc gagccgcaca tggccgtggt gttccatgac tcgaacaccc gcgcgtcggc
     1861 cgaggcgcaa tacgccaatt acatcgagta cggccgcaga ttggccggcc tgatcggcga
     1921 ggtggaaagg gagttgcagt tggcttgacg gcggccgcgg ctagccgccc agcttggtcg
     1981 gatcgctcac gtcgcccttg ttgaaatcga cgtactcctc cgtcagatcg gcggcgtaga
     2041 ggatcgcgct cgcagggccg aggttgagcc ggatgtgcag atcgaattcc ctgggagcca
     2101 ccgcggcgca gagttgcttg aacgacgtcg tggtcgggcg gccctttttg agactccaaa
     2161 ccaccgtcgg cgctccgggc gcgctgtacc cgatgtcgac gcgttcttcg acgattcgcg
     2221 ccgggctgta gccgagggca tcgatgatgc ggccccagtt cgggtcaccg ccaaaccaac
     2281 tggctttgac aagagcgctg ttggccaccg cgcgcacggc ggcgtccgct tccgcacagg
     2341 tcctggctcc ggataaccgg agtgtgacga agcgcgtgac gccttcaccg tcgcgcacga
     2401 tcattctggc caactcgagg cagacatgat ccaggccggc ctgaaatagc ccgatttccc
     2461 ggcttggctt cgccgcaggg cgccagcgca gttgctgatg gcctgcgagg ccattggcaa
     2521 gcacgagcac ggtgtcgttg gtgctcatgt cgccgtcgac cgtgatccgg ttgaagcttc
     2581 gggcgacggc atgggtcagc atcctttgca ggagcttcgc ctcgatggcg gcgtca
//
LOCUS       DAOQRO010000406         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_47461_length_2595_cov_4.512598, whole genome shotgun sequence.
ACCESSION   DAOQRO010000406 DAOQRO010000000
VERSION     DAOQRO010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47461_length_2595_cov_4.512598"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKK20_12395"
     CDS             <1..425
                     /locus_tag="PKK20_12395"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNV00730.1"
                     /translation="LGLVTNEYQEEFRRGDFLIRNLLGGDPSTGILAVGARPRMGQSV
                     QFQCRDAATATEDMIQTLRRVRRTLGERPVYGGCLCSCGGRGVRLFGTPHHDAALAQR
                     HFGPLGLAGFFGNGEIGPVGGRNYLHGYAASLALMVGK"
     gene            422..1096
                     /gene="trmB"
                     /locus_tag="PKK20_12400"
     CDS             422..1096
                     /gene="trmB"
                     /locus_tag="PKK20_12400"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNV00731.1"
                     /translation="MIQHTSPADPRSAVKPAPLLHPLTSIVDRLDWASVFPEKRPVEV
                     ELGAGDCSFLLELARLRPDHNFLGVERMLGRLRKLDRKGRRAGLTNLRGLRVEAAYCL
                     EYLLPLEGVEALHVYFPDPWPKRRHWRRRLVNERFPDLAARVLRPGGWIHLRTDHPDY
                     FQQMRHVFAPRTAFREVPVPDHLAALETDFEREFVARGVTILRLSYRCDTDGSPDAPR
                     TTGCVR"
     gene            complement(1149..2471)
                     /locus_tag="PKK20_12405"
     CDS             complement(1149..2471)
                     /locus_tag="PKK20_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00732.1"
                     /translation="MINETHRGLTRRQFLARGAAGAAALPCLIPASALGRDGAVAPSE
                     RIVMGGIGMGGRGAYDLSVLLGMPDVQWVAVCDVLKRQRQAAKNLVDNKMGNRDCAAY
                     SDMRQFLAERTDVDAVLIATGDRWHALASVLAMRAGKDVYCEKPACLTMAQGQMVAET
                     ARRYARVYQTGAQRLSEPHHVFAIEMARSGRLGPIHTAYADCRWRDGLRHDWLPAEAE
                     PSKDELDWDIWLGPCPWRPYNSGYVNGGGWYHFYDFATDVAMWGAHTVAQALAGLDMS
                     GVQSIEFEFAGPDQTMMTRLSNGVKLVLYRVPGSVWEKCEYWHGACGERFDGPEGWVA
                     AADGYSETDVSSPALLREYRKVLADYTARTQRPLNHVRDFIECIRSRRLTVAHHDVMA
                     QSMNICLAADICEQLKRNMTFDLRKAEFVGDAEANRLRSRAMRAPYLF"
BASE COUNT          449 a          885 c          762 g          499 t
ORIGIN      
        1 tgctcggttt ggtgaccaat gagtatcagg aggaatttcg gcgcggcgat ttcctgatcc
       61 gcaacctcct cggcggcgat ccctcgacag gcattctcgc ggtcggggcc cggcctcgca
      121 tgggtcagtc cgttcagttc cagtgccgcg atgccgccac cgcgaccgaa gacatgattc
      181 agactctgcg ccgagtccga cgcacattgg gggagcgtcc agtctatggc ggatgcctgt
      241 gcagttgcgg cgggcgcggc gtgcgcctct tcggcacccc acatcacgac gccgccctgg
      301 ctcaacggca cttcggtcct ctgggtctgg cagggttttt tggcaatgga gaaatcgggc
      361 ccgtgggggg gcgcaattac ctccacggct acgccgcttc tctggccctg atggtcggca
      421 aatgatccaa cacacttccc cggccgatcc gcgctcggcc gtaaagcccg cgccgcttct
      481 tcacccgctg acctcgatcg tcgatcgcct cgattgggcc agtgtctttc ccgaaaagcg
      541 tcctgtcgaa gtcgagttgg gtgcgggcga ttgctcgttc ctcctcgaac tcgcacgcct
      601 ccgtcccgac cataacttcc ttggcgtgga acgtatgctc ggccgattgc gcaagctgga
      661 tcgtaaaggc cgccgggccg ggctgaccaa tcttcgcgga ctgcgcgtcg aagcggctta
      721 ctgtctcgag tacctgctcc cgctcgaagg cgtcgaggct ctccacgtct acttcccgga
      781 tccgtggccc aagagacgcc attggcggcg gcgcttggtc aacgaacggt tccccgacct
      841 ggccgcccgc gtcctccgac ccggcggctg gattcacctc cgcaccgacc atccggatta
      901 tttccagcag atgcgccatg tgtttgcccc ccgcaccgcc tttcgcgaag tgccggtgcc
      961 cgaccatctc gccgcgctcg aaaccgattt cgaacgcgag ttcgtcgccc ggggtgtcac
     1021 aatcctgcgc ctgtcttatc ggtgcgatac ggatggcagt ccggatgcgc cgcggacgac
     1081 cggctgtgtg cgatgaaggc cctgcaacta catcgacaag catcgagaat gtccaaactc
     1141 cccgtgggct aaaacagata cggcgctcgc atcgcgcggg accgcagccg gttggcctcc
     1201 gcatccccga cgaattccgc cttgcggaga tcgaacgtca tgttgcgctt gagctgctcg
     1261 cagatgtcgg cggcaagaca gatattcatt gactgcgcca tgacatcatg gtgcgcgacc
     1321 gttagccgac gcgatcgaat gcactcgatg aaatccctta cgtgattcaa gggccgctgc
     1381 gtccgggccg tgtagtcggc cagcactttc ctgtattcgc gcaacaacgc cggagacgag
     1441 acatccgtct ccgaataacc gtcggccgcc gcaacccaac cctcgggccc gtcgaatcgc
     1501 tcgccgcacg ccccatgcca gtactcgcac ttctcccaaa ccgacccggg cacgcgatag
     1561 agaaccagtt tcaccccgtt cgacaaacgg gtcatcatgg tctgatcggg acccgcaaac
     1621 tcgaactcga tcgactggac gccgctcata tcgagcccgg ccagggcctg ggccactgtg
     1681 tgggcgcccc acatcgcaac gtccgttgcg aagtcgtaga agtgatacca cccgccgccg
     1741 ttgacgtagc cgctgttgta agggcgccag gggcaggggc ccaaccagat gtcccagtcg
     1801 agttcatcct tggacggctc ggcttccgcc ggcaaccagt cgtgacgcaa cccgtcgcgc
     1861 cagcggcagt cggcgtaggc ggtgtggatt ggtcccaagc ggcccgagcg cgccatctcg
     1921 atggcgaaca cgtgatgcgg ttcgctgagc cgttgcgccc ccgtctggta cacgcgcgcg
     1981 tagcggcggg cggtttcggc gaccatctgt ccctgggcca ttgtcaggca ggccggtttc
     2041 tcgcagtaga cgtctttgcc ggctcgcatg gccagcaccg acgccaacgc gtgccagcgg
     2101 tcgccggtgg caatcagaac cgcatccaca tccgtccgct cggccagaaa ctggcgcatg
     2161 tcgctgtacg cggcgcagtc ccgattcccc attttgttgt ccaccaggtt cttcgcggcc
     2221 tggcgctggc gcttgagaac atcgcagaca gcgacccact ggacatcggg catgcccaac
     2281 aacacactca agtcgtaggc gccgcgtccg cccatgccga tcccacccat cacgatccgt
     2341 tcgctcgggg ccaccgctcc gtcgcgtccc agcgcggagg ccgggatcag gcacggcagc
     2401 gccgccgcac ccgccgcgcc tcgggcaaga aactgtcgcc tcgtgagtcc tctgtgcgtc
     2461 tcgttgatca tatgcctctt tgctttgtca ggtcggttga cggaacgtgg tacccccacc
     2521 ctgaattgaa cagggatcaa cggtttagga agccaactgt gaatttgcat atttccaaac
     2581 aaatataggg ttttt
//
LOCUS       DAOQRO010000407         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_47489_length_2594_cov_5.095707, whole genome shotgun sequence.
ACCESSION   DAOQRO010000407 DAOQRO010000000
VERSION     DAOQRO010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47489_length_2594_cov_5.095707"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKK20_12410"
     CDS             complement(<1..340)
                     /locus_tag="PKK20_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00733.1"
                     /translation="MNILRHLGRRRSGFTLIELLVVIAIIAILAGMLLPALGKAKSKS
                     HQIHCLNNTKTLVLATFMYLNDFNAPVPYNGTATATMDNALWVTVLATNYGGINAARI
                     CPAAPSAPTKA"
     gene            complement(402..1688)
                     /locus_tag="PKK20_12415"
     CDS             complement(402..1688)
                     /locus_tag="PKK20_12415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV00734.1"
                     /translation="MKNVSNVLALCGGTPVCTGSWPPWPVFDDTERRLLSGVLESGKW
                     WFGERVRQFEAEFAAFQGASHAVTCTNGTTAIEMGLKALGVLPGDEVIVPPYSFIATA
                     SAVVTLGAVPVFADIRPDTLCLDPADVACKVTPRTRAIIPVHVGGCIADMEAINAVAR
                     AHGLRVLEDAAHAWGSQWKGRGAGVLGQCGTFSFQVSKNITAGEGGVLVTDDGELADM
                     CRSYTHCGRRKGAAWYDHDYLGSNLRLTEFQAAILIAQLTRLEAQIRCRQAAASILDR
                     ALAGLAGIRLLAPAPEMTRRSYHMYILRLDESALGVSRDLFVEALIAEGVPASGGWYR
                     PLYANGVFRNPSGEPLHGIRAPVADRASEYARTCCPVCEQVCRDAVWIPQNVLLSGAD
                     RIGEVARAIGKVVANAEALRVESRRRGCDRKGAAAS"
     gene            complement(1936..>2594)
                     /locus_tag="PKK20_12420"
     CDS             complement(1936..>2594)
                     /locus_tag="PKK20_12420"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV00735.1"
                     /translation="LDALRTLRDRSFAVFEITAFLFCIPLTLYWGFATMFLQDMGVAN
                     VVGTMTLGQASQIVFMLLLPLVLGRLGVKWVFVLALLGWATRYCLFHLGYEGAGAWSL
                     YLGVALHGVCYVFFFVLAYIYVDQEAPEAIRTRAQGFISLVTLGLGFFVGSIVAGWTV
                     QQFSFPNAETIASGEVSRPITLWGRVWLAGSLSSFILMMPFLLVFRHDRQKGNGTASK
                     "
BASE COUNT          544 a          875 c          761 g          414 t
ORIGIN      
        1 tggctttcgt gggggccgac ggagccgccg ggcaaatgcg tgccgcgttg atgccgccgt
       61 aattggtggc caggaccgtg acccacaggg cgttgtccat cgtcgccgtg gccgtgccgt
      121 tgtaaggcac aggcgcattg aagtcgttca ggtacatgaa cgtcgcaagc accagcgtct
      181 tggtgttgtt caggcaatgg atctgatggc ttttggattt ggccttgccc aaggccggca
      241 aaagcatgcc cgccagaatg gcaatgatag ctatgacaac cagcaactcg atgagcgtga
      301 atcccgaacg acggcgcccc aagtgtcgga gtatgttcat aagtcgagag tcattctgac
      361 acgtccgacg gattggtcaa ggttcggact caaggcgctc ttcaagacgc cgcagcacct
      421 ttccgatcgc acccccggcg cctcgattcc acccgcaacg cctccgcatt ggcaaccacc
      481 ttcccaatcg cccgggcgac ttcgccaatc cggtccgccc cggacagcag cacgttctgc
      541 ggtatccaga cagcatcccg gcacacctgc tcgcacaccg gacagcaggt tcgggcgtac
      601 tcggacgcgc ggtcggcgac tggagcgcgg atgccgtgaa gcggttctcc ggaagggttg
      661 cggaacacgc cgttggcata gagcggacga taccagccgc cggaggccgg cacgccctcc
      721 gcgatcagcg cctcgacgaa taggtcgcgg ctcacaccga gcgcggattc gtcgagccgc
      781 aagatgtaca tgtgatagct gcgccgcgtc atctcgggcg ccggggccag cagccggatc
      841 ccggcaagtc cggccaacgc gcgatcgaga atggacgcgg cggcttggcg acaacgaatc
      901 tgcgcctcga gtcgcgtcaa ctgcgcgatc aaaatggcag cttgaaattc cgtcagccgc
      961 aagttgctgc cgaggtagtc gtggtcatac caggccgccc ctttccgccg cccgcagtgc
     1021 gtgtagctgc ggcacatgtc cgccaactcc ccgtcgtccg tgaccaggac cccgccctcc
     1081 ccggccgtga tattcttcga gacctgaaaa ctgaacgtgc cgcactggcc aagcaccccg
     1141 gctccgcgcc ccttccactg gctgccccag gcgtgcgccg cgtcctcgag cacgcgcaag
     1201 ccatgcgccc gcgccaccgc attgatcgcc tccatgtcgg caatgcaacc cccgacatgc
     1261 accgggatga tcgcccgcgt gcgcggcgtg accttgcagg caacatccgc cgggtccaaa
     1321 cagagtgtgt caggccggat gtcggcaaac acggggaccg ccccaagagt gacgaccgcg
     1381 ctggcggtgg cgatgaacga atacggggga acgatcacct cgtcgccagg caacacccca
     1441 agggccttca gccccatctc gatcgccgtc gtgccgttgg tgcaggtcac ggcgtgactc
     1501 gccccctgaa acgccgcgaa ttccgcctcg aactgccgaa cccgctcgcc aaaccaccat
     1561 ttcccagact cgagcacgcc gctcagcagc cgccgctccg tgtcgtcaaa caccggccag
     1621 ggcggccatg acccggtgca aacaggcgtt ccgccgcaca gagcgaggac attcgaaaca
     1681 tttttcatcg gtattcgctc cacagcccgg acatcagacc acgcgcagcc ttgcgaaatg
     1741 tccgggctaa ccccgcatgc ttcacacccc gatggtgcac aggtcactca gacttcgcaa
     1801 gaagactgcg atgcggctta gcaagctgac aaatgcatgc gcgccatctt gtcttccggg
     1861 ccatccggga agaggctggg cggcgccgcg gatcgcaaac gctgtgttgc gatgccccat
     1921 tgagcgattt gtcaactatt tagatgcggt cccattgcct ttctggcggt cgtgccggaa
     1981 gacgaggagg aacggcatca tcaggatgaa gctgctcaaa gacccggcca gccagactct
     2041 gccccagagc gtgatcggtc gtgagacctc gcccgacgcg atcgtctcgg catttggaaa
     2101 gctgaactgt tggaccgtcc aaccggcgac gatcgatccg acaaagaagc ccaggccgag
     2161 cgtgacgagc gagatgaacc cttgcgccct ggtgcggatc gcctcgggcg cctcctgatc
     2221 gacgtagatg taggccagca cgaagaagaa cacgtaacag acgccgtgca gcgccacgcc
     2281 cagataaagc gaccaggcgc cagccccttc gtagccgagg tgaaacaggc aataccgagt
     2341 cgcccaaccc agcagggcca ggacgaacac ccacttcacc ccgaggcgtc ccagaaccaa
     2401 cggcaggagc agcatgaaga cgatctgcga cgcctggccg agtgtcatcg tgccgacgac
     2461 attcgccact cccatgtcct gcagaaacat cgtggcgaaa ccccaataca gagtcagtgg
     2521 aatgcagaaa aggaaggccg taatctcaaa caccgcgaac gaacggtccc tcagcgtgcg
     2581 caacgcgtcc aatc
//
LOCUS       DAOQRO010000408         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_47676_length_2588_cov_5.227004, whole genome shotgun sequence.
ACCESSION   DAOQRO010000408 DAOQRO010000000
VERSION     DAOQRO010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47676_length_2588_cov_5.227004"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKK20_12425"
     CDS             <1..205
                     /locus_tag="PKK20_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00736.1"
                     /translation="ERRAVAVVVIGGQALCLLLTLIVTPIAYSFLDDLGRKLQWRGWR
                     IETDSAPATAPAGMEMARVKSDE"
     gene            450..1019
                     /locus_tag="PKK20_12430"
     CDS             450..1019
                     /locus_tag="PKK20_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00737.1"
                     /translation="MTEGLIYAMVLFSVWAVASLPAWAVWTMNGAGYALGLLLTIKRL
                     TSRVTGYQPARWGEGAVRWPMRLLGALTVVLVLWCLISALNPRATVDLAQLRLGFRDG
                     TIPWLPHSCDAPSSWFAFWQYLGLACGFWAIRDWLLGTTRRERHDREPSPGSTSPNSE
                     LRTPNSELRTPNSELRTVTGLWPRACRPG"
     gene            992..2527
                     /locus_tag="PKK20_12435"
     CDS             992..2527
                     /locus_tag="PKK20_12435"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV00738.1"
                     /translation="MAEGLPARLKRLLWVLCLNGTAVAIVGILQRLSGTTKLLWIFEP
                     AHAGSNRTQFGPFDYYANAAQYLNLLWPVCLAFWWYLHHARRNSVRRAARVGDSPHVL
                     LLPSAFLLFGAAIFAGSRGGALVASALFVVSAGLVLAGSTPHVRKIGIVLVGLLGLAG
                     AGAARLGGWQAMNRLLEPPTHFSTGVQGGLSDFSLHFVIDCPTEGAARWWSLAYLVSD
                     SIDAGQNSAILYVYKDTLGFLLRGNPATNQIRVVCTNFLPLFGGRRVAVSAVRSQTNL
                     ALYAEGDPLRVVTSRSRFPPLTNRISASVLCVQRDRDPMRPSDTPTVHHAQLYAEALS
                     QMQVRALISSSGQRNGDQNGTNHPPAPCLDISRKTLTGPKSLSAQLQDRTEIHELGRR
                     IAADYPWFGSGPGTFSSMYEVYRRTRLTRPWYVHNDWLETRITFGRIGALLVFCALAM
                     AVLRPLLHGGPRVSAVFIGLVWAGLGGCLVHARFDYPLQVHSILFTFLVASAATSIVS
                     VRK"
BASE COUNT          412 a          807 c          818 g          551 t
ORIGIN      
        1 cgagcgccgc gcggtggcgg tggtggtgat cggggggcag gccttgtgcc tgctgctgac
       61 actgatcgtc actccgatcg cctacagctt tctcgatgat ctcgggcgca aactccaatg
      121 gcgtgggtgg cgcatcgaga ccgacagcgc gcccgccacc gcgcctgccg gcatggaaat
      181 ggcgcgagtc aagagcgatg agtgatcggc tcggatcgga gatggagctc ccgggatcga
      241 aggcgcacgc aatgggcgag agggcaagcc cggatttctc ccaaggctcc gcgcgatctg
      301 atgctgtgcc tgtgtcaagg ccaagccatg tccaagtggc gcacggttcc gatgccgccc
      361 ggtcattcgc atcccgctcg ggtcattctt cccgccgcgt tcatgcccac gagaccgctg
      421 cgccccgtct ctatcagtgg gccgacagag tgacggaagg gctgatctac gccatggtcc
      481 tgttctccgt ctgggccgtt gcttctctgc cggcttgggc ggtttggacc atgaacggag
      541 cagggtacgc tctgggtctt ctcctgacga tcaaacggct gaccagccgg gtcacaggct
      601 atcagccggc tcgatggggc gagggcgcag tccgatggcc gatgcgcttg ttgggggctc
      661 tgacggtggt tctcgtgctt tggtgcttga tcagcgcctt gaacccgcgc gccacagtcg
      721 acctcgccca actccgtctt ggctttcgtg acggcacgat tccctggctg ccgcacagtt
      781 gtgatgcccc gagcagttgg ttcgcttttt ggcagtactt gggcctggcc tgcggcttct
      841 gggccattcg cgattggctg ttgggaacga cccgacgcga acgccatgac cgcgagcctt
      901 cacccggttc gacatccccc aactccgaac tccgaactcc gaactccgaa ctccgaactc
      961 cgaactccga actccgaacc gtcacagggc tgtggccgag ggcttgccgg cccggctgaa
     1021 gcggctgctg tgggtgcttt gtctcaacgg caccgctgtg gcaatcgtgg gcatcctaca
     1081 gcgactttcg gggacgacca aactgctctg gattttcgag cctgcgcatg cggggagcaa
     1141 ccgaacgcaa ttcggtccat tcgactatta cgccaacgcc gcccagtacc tgaacctgct
     1201 ctggcccgtc tgtctcgctt tctggtggta tctgcaccat gcccgaagaa actcggtgcg
     1261 ccgcgcggct cgcgtcggcg attcgcctca tgtcctcctg cttccatcgg cgttccttct
     1321 gtttggcgcg gccatctttg cgggctcgcg cgggggcgct ttagtggcct cggccctgtt
     1381 cgttgtctcg gcgggtctgg tgttggcagg gagcacgcca catgtgcgga agattgggat
     1441 tgtccttgtg ggcttgctgg gccttgcggg cgccggggcc gcccgattgg gcggatggca
     1501 ggcgatgaat cgattgctcg aaccgccaac gcattttagc acgggcgtcc agggaggttt
     1561 gtctgacttc tctctgcatt tcgtcatcga ctgcccgacc gagggcgccg cgcggtggtg
     1621 gagcctggcc tacctagtgt cggattcgat cgatgccggg cagaactcgg cgattctcta
     1681 cgtctataag gacacgctcg gttttctgct tcgcggcaat cctgccacca accaaatccg
     1741 cgtggtctgt acgaacttcc ttcccctgtt tggcggacgc cgtgtggctg tgagcgcggt
     1801 gagaagccag acgaatctgg cattgtatgc cgagggcgac ccgctgcggg ttgtcacgag
     1861 tcggtcgcga tttccacctt tgaccaaccg gatcagcgca tcggtgctgt gcgtgcagcg
     1921 ggatcgggac ccgatgcgcc cttcggacac gcctaccgtg catcacgccc aactctatgc
     1981 ggaggcgttg tcgcagatgc aggtgcgcgc cctgatcagc tcatccgggc aacgaaatgg
     2041 cgatcagaat gggacgaatc acccgcctgc cccctgcctc gacatctccc gcaagacctt
     2101 gaccggaccg aaatccctca gcgcacaact ccaggaccga actgaaatcc acgaactcgg
     2161 acgccgcatt gccgccgatt atccctggtt cggctcgggc ccggggacgt tcagttcgat
     2221 gtacgaggtc taccgccgga caaggctcac ccggccgtgg tacgtccaca acgattggct
     2281 cgaaacgcga atcaccttcg gacgcatcgg cgcattgctg gtgttttgtg ccctcgcaat
     2341 ggcggtcctg cgtccgttgc tgcacggcgg tccccgtgtc tcagcggtct ttattggcct
     2401 ggtctgggcc gggctgggcg gatgcctcgt ccatgcgcgc ttcgattatc cgcttcaagt
     2461 ccactcgatc ctcttcacct ttcttgttgc aagcgccgcc acctcgattg tgtcggtgag
     2521 gaagtaggag atgggagatg ggagatcgga gatgggagat cggagatggg agatcggaga
     2581 tgggagat
//
LOCUS       DAOQRO010000409         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_48062_length_2574_cov_4.580786, whole genome shotgun sequence.
ACCESSION   DAOQRO010000409 DAOQRO010000000
VERSION     DAOQRO010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48062_length_2574_cov_4.580786"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>2574)
                     /locus_tag="PKK20_12440"
     CDS             complement(160..>2574)
                     /locus_tag="PKK20_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNV00739.1"
                     /translation="LETPAHRVSGRFGLRDFGVDGTQFTVNGRKTFLRGTLECAIFPK
                     TGHTPTDRETWRRVMRAAKAHGLNNLRFHSHCPPEAAFAAADEEGVYLHVECGTWANQ
                     STTLGDGKPVDAWVYREAERILSAYGNHPSFVMLLYGNEPGGDRHAAYLKAWVEHFRD
                     RDERRLYSSGAGWPQIPENQFHVVPDPRIQAWGGGMQSRINARPPETVTDYRDFIRAR
                     PVPVISHEIGQWCVYPNFAEIPKYTGYLKARNFEIFRDSLERGGMLDQAEAFLFASGR
                     LQTLCYKEDIESALRTPGMGGFQLLDLHDFPGQGTALVGVLDPFWESKGYVTADEYRR
                     FCNSTVPLARLAKRVFTTDEELVAGLEVAHYGPRPIEGATLVWRLVDDKGRALAESTS
                     SCPEIPLGSATPLGSVRVPLESMPAPARTKLVVSIEARGDQAAARRAAASFRGVRFEN
                     DWDIWVYPPAVSTALPADVTVTETLDENAKVVLGRGGKVLLVVPPSRVRPDPKRGRIA
                     LGFSSIFWNTAWTGRQPPHTLGILCNSRHPAFADFPTDGYSNWQWWYLVSRAGAMILD
                     DLPSDLRPTVQVIDDWFTNRRLGLVFEARVGSGRLLVSSIDLRCDLESNPVARQLRHS
                     LLNYMASQAFRPKVECSIAQIEGLMAPESLMQKLGAKVHRADSAHTGHDAALALDGDP
                     RTMWHTPWGEGAPGFPHEIQIVFERAVRLGGIRLTPRLDGNPNGTIKDYAVYVSPDGQ
                     NWSEPVARGTLAPGDAVQAIEIAPTPPVRFVRLVALSGHARGPWASLAEFDVWLAVPE
                     GENGDE"
BASE COUNT          472 a          861 c          818 g          423 t
ORIGIN      
        1 agacgttttc atggcgtgac ggcgcaatca tgcgcccggc agcgcagcgt ggaaagccaa
       61 aacgcggcac tctgattgtg accaggttca aagcgccggg gcgggagaac cctggcgcaa
      121 caccggggtg cacccggcga gttcatcggt cgcgggtcat cactcatcgc cgttctctcc
      181 ctccggcaca gccagccaca catcgaactc ggccagcgag gcccacggac cgcgcgcgtg
      241 gccgctgagc gccaccaggc gcacaaaccg cactggcggc gttggcgcga tctcgatcgc
      301 ctgcaccgca tcgcccggcg caagcgtccc gcgcgcgacc ggttcgctcc agttctgccc
      361 atcggggctg acgtagacgg cgtagtcctt gatggtccca ttcgggttgc catcgaggcg
      421 cggcgtcaga cgaatccctc ccaaccgcac ggcccgttcg aagacgattt gaatctcgtg
      481 cggaaatccc ggggcgcctt cgccccaggg cgtgtgccac atggtccggg gatcgccatc
      541 cagagcgagg gcggcgtcgt ggccggtgtg ggcgctgtcc gctcgatgca ccttggcgcc
      601 gagtttctgc atgagcgact cgggcgccat gagcccttca atctgcgcga tcgagcactc
      661 gaccttcggg cgaaaggcct gactggccat gtagttcaac agactgtgcc ggagttggcg
      721 ggccactggg ttggattcaa ggtcgcaacg gaggtcgatg ctcgagacca gcagcctgcc
      781 cgagccaacc ctggcttcaa aaaccaggcc gagccggcga ttggtgaacc aatcatcgat
      841 gacctgaacg gtcgggcgca ggtccgacgg aaggtcgtcg aggatcatcg cgccggcccg
      901 gctgacgaga taccaccact gccagttact gtaaccatcg gtgggaaaat cggcgaaagc
      961 gggatggcgc gaattgcaca gaatgcccag agtgtgaggc ggttggcgcc ccgtccatgc
     1021 cgtattccag aaaatgctcg aaaacccgag cgcgatgcgt ccgcgcttcg gatcgggccg
     1081 cacccggctt ggaggcacga cgagcagcac cttgcccccg cgacccaaga cgaccttggc
     1141 attctcgtcg agcgtctccg tgaccgtgac atccgccggc agcgcggtcg acacggccgg
     1201 cgggtagacc cagatgtccc agtcgttctc gaatcgaact ccgcggaaag aagctgcggc
     1261 acggcgggcc gccgcctgat ccccgcgcgc ttcgatcgag acaacgagct tggtgcgcgc
     1321 cggggcgggc atcgactcga gtggcacgcg gacactccca agaggcgtgg cgctgcccag
     1381 cggaatctcg gggcaactcg aagtcgattc ggccagcgcg cggcccttgt catcgaccag
     1441 ccgccagacc agagtcgcgc cttcgatcgg gcggggaccg taatgggcga cttccaaacc
     1501 ggccaccaac tcctcgtccg tcgtgaagac gcgcttggcg agccgcgcca acggcacggt
     1561 cgaattgcag aaccggcggt actcgtccgc cgtcacgtag cccttggact cccagaacgg
     1621 atcgagcacg ccgaccagcg ccgtgccctg cccggggaaa tcatgcaaat cgagcagttg
     1681 aaagccgccc atcccgggcg tgcgcagagc cgactcgatg tcctccttgt aacagagcgt
     1741 ctggagtctg cccgacgcga acaggaacgc ctcagcctgg tcgagcattc cgcccctctc
     1801 gagcgagtcg cggaagatct cgaagttgcg cgctttcagg tagcctgtgt atttcggtat
     1861 ctcggcgaag ttgggataga cgcaccactg gccgatctca tggctgatga ccgggacggg
     1921 acgcgcccgg atgaagtcgc ggtagtcggt gaccgtttcc ggaggacgcg cattgatccg
     1981 cgattgcatg ccgccgcccc aggcctggat gcgcgggtcc ggaaccacat ggaattggtt
     2041 ttcgggaatc tgcggccaac ccgctccgct cgagtagagc cggcgctcgt cccggtccct
     2101 gaaatgctca acccacgcct tgagatacgc cgcgtgccga tcgcccccag gttcgttccc
     2161 ataaagcaac ataacaaacg acgggtgatt cccataggcg ctcagaatcc gttcggcttc
     2221 gcggtacacc caggcatcga ccggcttgcc gtcccccagt gtcgtggatt gatttgccca
     2281 ggtgccgcac tcgacatgaa gatagacgcc ctcttcgtcc gccgccgcga acgccgcctc
     2341 gggcggacag tgcgagtgga agcgcaggtt gttgaggcca tgcgccttgg ccgcccgcat
     2401 cacccgccgc cacgtctcac gatcggtcgg cgtgtggccc gttttcggaa agatggcgca
     2461 ctcgagcgtg ccgcgcaaaa acgtcttgcg cccgttgacg gtgaactgcg tgccgtccac
     2521 gccaaaatct cgcagcccga accgtccgga cacgcggtgg gctggcgtct ccag
//
LOCUS       DAOQRO010000410         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_48240_length_2568_cov_4.216872, whole genome shotgun sequence.
ACCESSION   DAOQRO010000410 DAOQRO010000000
VERSION     DAOQRO010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48240_length_2568_cov_4.216872"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKK20_12445"
     CDS             complement(<1..846)
                     /locus_tag="PKK20_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00740.1"
                     /translation="MQTQRIDRRHFVIRGGLGLLGALTSPDLARASQNADSDRWDPAR
                     PFPAIGRKLRIQPVLMYHLPTRRDQASWRSWGGVQTEAAVTEEIGRIERELLEIGRRA
                     EFPHEFLPLAPVTSIDAARALAQRDSDVTLVYACTGGGNLLEACLAAKPDALIFVRHR
                     SGPVYYWYEALSTRFLARRDRGGVTTGRSPTPAAHLDDVVVDDPKEVLWRLRALFAVN
                     NLRGVITQLSAEIASLKSSIDNNAKASNAQIAKIVERVDRAQAEPAQRIQKLTEQVEK
                     LDRRIV"
     gene            944..2278
                     /gene="purB"
                     /locus_tag="PKK20_12450"
     CDS             944..2278
                     /gene="purB"
                     /locus_tag="PKK20_12450"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNV00741.1"
                     /translation="MITRYSRPAMRGIWTEENKLRIWLQIELLATEALVAEGIVPASD
                     FARIRVGVDRWLGDLEGLVRRQKELEKTLNHDVIGFTTAVAEAIDDPASRWFHFGLTS
                     SDVIDTAFALQMVQSADILIEDVKALRQVLARRAREHQFTPCIGRSHGIHAEPTTFGL
                     KLALMHDEFGRHLRRLESARQTVGVGKLSGAVGTNAHLSPGVETAVCARLGLRPAPIA
                     TQVVSRDIHAEFQAALAIVGAGIERWAVEFRHLQRTEVLEAEEPFGKGQKGSSAMPHK
                     RNPITWERLTGLARVLRGNALAAFENVALWHERDISHSSVERIIFPDSCTLLDYMFGL
                     LIRLIEGLNVYPDNMRKNLGLSLGMWNSQTVLLALISKGLTREEAYALVQDAAMKTWE
                     VKHAGRDDADFLAQLKANPAVSSHFEQGELERLCSIDFHLKAVEDRFRTLGL"
     gene            2360..2548
                     /locus_tag="PKK20_12455"
     CDS             2360..2548
                     /locus_tag="PKK20_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00742.1"
                     /translation="MKPILSVDAPNGGFQRPVIDYSGVENPVDGSSARQHRAGTPAVA
                     PRGRRDEGPAFTLIELLV"
BASE COUNT          459 a          846 c          809 g          454 t
ORIGIN      
        1 gacgatacgg cgatcgagct tctcgacctg ctccgtcagc ttctggatgc gctgcgccgg
       61 ctcggcctgc gcgcggtcga cacgctcgac gatcttggcg atctgcgcgt tcgaggcttt
      121 ggcgttattg tcgatcgagg atttcagcga agcgatttcc gccgacaatt gggtgatcac
      181 gccgcgcagg ttgttgaccg caaagagcgc gcgcaaccgc cagagcactt ccttcggatc
      241 gtccacgaca acgtcgtcca ggtgcgcggc cggcgtcggg ctgcggcccg tcgtcactcc
      301 accccgatct cgccgcgcca ggaaccgcgt gctcagggcc tcgtaccagt aatacacggg
      361 tccggagcga tgcctgacga agatcaaggc atcgggcttg gccgccagac aggcctcgag
      421 gaggtttcca ccgcccgtgc aggcgtacac cagcgtcaca tccgagtccc tttgcgcaag
      481 ggcgcgcgcc gcgtcgatgg acgtcaccgg cgccaggggt aggaattcgt ggggaaactc
      541 ggcgcgccgt ccgatctcca acaactcgcg ctcgatgcgc ccgatctcct cggtcaccgc
      601 cgcctccgtc tgcacaccgc cccaggacct ccaggaggcc tggtcccggc gagtcggcag
      661 gtgatacatc aggacgggct ggattcgcaa tttgcgtccg atcgcaggga aaggtctggc
      721 cggatcccat cggtcggagt cggcgttttg ggaggcacgc gcaaggtccg gcgatgtcag
      781 cgcgccgagc aagcccaggc ctccccgaat gacaaagtgt cgacggtcga ttcgttgcgt
      841 ttgcatgtga gttgacgcgc caaaccctgc gctttcctcc gggatcgtca accgcaattc
      901 cattgggctc gactggtcgg ttcccctgcc ctaccctcat ctcatgatca cacgctattc
      961 gcgtccggcc atgcggggca tctggaccga ggagaacaaa ctgcgcatct ggcttcagat
     1021 cgaattgctt gccacggagg ccctggtggc tgaagggatc gtgccggcgt cggacttcgc
     1081 cagaatccgt gtaggggtcg accggtggct gggcgatctc gaagggctcg tcagacgcca
     1141 gaaagaactc gagaaaacgc tgaatcacga cgtcatcgga ttcacgactg ccgtcgccga
     1201 ggcgatcgac gatcccgcca gccgctggtt ccacttcggc ctcacctcga gcgatgtgat
     1261 cgacaccgcg ttcgccctcc aaatggtcca atcggccgac attctcatcg aggatgtcaa
     1321 ggcgctgcgc caggtcctgg cccggcgggc gcgcgagcac cagttcacgc cgtgcatcgg
     1381 acgcagccac ggcattcacg ccgagccgac cacgttcgga ctcaagctcg cgctgatgca
     1441 cgacgagttc ggtcgccacc tgcgccggct cgagtcggcc cgacaaaccg tcggcgtcgg
     1501 caaactcagc ggggccgtgg gcaccaacgc ccatctctcg ccgggggtcg agacggctgt
     1561 gtgcgcgcgg ctcggcctga ggccggcccc gatcgcgacg caggttgtgt cccgcgacat
     1621 tcacgccgag ttccaagccg cgctggcgat cgtgggggcc ggcatcgaac gctgggcggt
     1681 cgagttccgc catctccagc gcaccgaagt gctcgaggcc gaggagccct tcggcaaggg
     1741 gcaaaagggc tcgagcgcga tgccccacaa gcggaatccc atcacatggg aacggctcac
     1801 cgggctggcc cgggttttgc gcggcaacgc cctcgccgca ttcgagaacg ttgcgctgtg
     1861 gcacgaacgg gacatcagcc acagctcggt cgagcgcatc attttccccg attcctgcac
     1921 gttgctcgat tacatgttcg gcctgctgat ccggctcatc gagggcttga acgtctatcc
     1981 ggacaacatg cgcaagaacc tcgggctcag cctcgggatg tggaacagcc agacggtgct
     2041 gctggcgctg attagcaaag gcctcacacg cgaggaggcg tacgccctgg tgcaggacgc
     2101 cgcgatgaag acttgggaag tgaagcacgc cgggcgcgac gacgccgatt tcctggcgca
     2161 gctcaaggcc aacccggcgg tctcaagcca cttcgaacag ggcgaactcg aacggctttg
     2221 ttcgatcgac ttccatctca aggcggtcga agaccgcttt cgcactctgg gattgtgaat
     2281 gcgcgaggcc cgctccgggc caagtttgcg attgccagcc atggcctttg cgccgaaaag
     2341 aacgtgaacc actgagtcta tgaaaccaat cctttccgtg gatgcgccga atggcggctt
     2401 tcagcgccca gtcatcgact attctggagt ggagaacccg gtggatggct cgagcgcccg
     2461 ccaacaccgc gcaggcacac cggccgtcgc ccctcgcggt cggcgggacg aggggcccgc
     2521 cttcactctg atcgagttgc ttgtgtgatt gcgatcatcg ccatcctt
//
LOCUS       DAOQRO010000411         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_48565_length_2557_cov_3.973221, whole genome shotgun sequence.
ACCESSION   DAOQRO010000411 DAOQRO010000000
VERSION     DAOQRO010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48565_length_2557_cov_3.973221"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKK20_12460"
     CDS             <1..244
                     /locus_tag="PKK20_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00743.1"
                     /translation="QLSSPDMLVTGVKPSDDGQALIFRFWGAAGREAQTEIGWNGFSA
                     RQLWHSDASEGPLRKLEGRVTVPAWGLTTVRADLEI"
     gene            247..1182
                     /locus_tag="PKK20_12465"
     CDS             247..1182
                     /locus_tag="PKK20_12465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNV00744.1"
                     /translation="MGSEVSFLKRIGTRIGLAALCAAWFFLGPSPILGAVAPGGATAK
                     PSEKSAPRPAPARSIPRDPYVGAIVVDVGAKRVLFEDRADVQGYPASVLKLMDLLIIL
                     ERIERGQLSFQDKVPVSAKAAATGGSQVWLAEREVFTVDEMLYALMVRSANDAAVALA
                     EKVAGSTSAFVGLMNQRARELGMTNTRFNSVHGLPPGPGQPPDVTTARDLARLCYAVL
                     QKRDALRYTSTRQRTFRPASSKSAVVMRNHNPLLAQVAGCDGLKTGYIAAAGFSIAVT
                     AQRQDRRLVAVVLGSADRKRRDAKAVELIERGFAN"
     gene            1338..2255
                     /locus_tag="PKK20_12470"
     CDS             1338..2255
                     /locus_tag="PKK20_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNV00745.1"
                     /translation="MNATVCAGIEVWSMRTRLCEFTLGLAVIASAVGLDLFCALGQPA
                     PGESAPRSKFTLACRLANYQGCEQAAWTHLPAIGVRHVFMSVPAADQVEIVRKRLADH
                     GLKAVVFRGDADLARPEGVDQLDGQLAVCERMGVKYLFLSAKSRDVPKALVYQRLRRA
                     GDLAARHGVTIAIETHPDLGTNGDVLLETMRQVDHPCVRVNFDTGNIHFYNDGMDAPT
                     ELRKILPFVATVEIKDHDGGVGEWRFPALGRGRVNIAAVLQILTEHGYSGPITMEIEG
                     VKDVSRTPQQIQEDIAQSAAYLRTLAAFE"
     gene            2291..>2557
                     /locus_tag="PKK20_12475"
     CDS             2291..>2557
                     /locus_tag="PKK20_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00746.1"
                     /translation="MITPVASRTLRLALFTLALSLCAGSKTMAGTAGQRASGRLIARI
                     HDSGFPQGHDTYNGMLAASDGRIYYVLCSEAYIWPARCTRTIPRR"
BASE COUNT          441 a          783 c          840 g          493 t
ORIGIN      
        1 ccaactgagt tcgccggaca tgctggtgac cggtgtcaag ccgagcgatg acggccaagc
       61 gctcatcttt cggttctggg gcgccgccgg acgcgaggcg cagaccgaga tcggatggaa
      121 cggattctct gcacggcagc tctggcatag cgacgccagc gaaggacctt tgcgcaagct
      181 cgaaggccgc gtcaccgtcc cggcctgggg tttgaccacc gtgcgggcgg atctggagat
      241 ctgagtatgg gaagcgaagt ttcttttcta aaacgcattg ggactcgcat cggcctcgcg
      301 gcgctctgcg cggcgtggtt cttcctcggt ccgtcgccaa ttctcggcgc ggtcgcgccc
      361 ggcggcgcga cggccaagcc ttccgaaaag tcggctccaa ggcctgcgcc cgcccggtcg
      421 attccgcgcg atccgtatgt gggcgcgatt gtcgtggacg tgggcgccaa gcgggtgctg
      481 ttcgaagatc gggcggatgt ccaaggttac cccgcgagcg tgctgaagct gatggatttg
      541 ctgatcatcc tcgagcggat cgagcgcggg cagctttcgt ttcaggataa ggtcccggtg
      601 agcgcgaagg cggccgcgac gggcggctcg caagtgtggc tcgccgagcg ggaggtgttt
      661 accgtggacg aaatgctcta tgcgttgatg gtcaggtcgg cgaatgacgc cgccgtggct
      721 ttggccgaga aggtcgccgg aagcacgtcg gcttttgttg gcttaatgaa ccagcgtgcc
      781 cgggaactgg gcatgaccaa cacccgattc aactccgtgc acggtcttcc gccaggccct
      841 ggccagccgc ctgacgtgac gacggcgagg gacctggccc gcctctgcta tgccgtcttg
      901 cagaaacggg atgcgttgcg ctacacctcg acgcgtcaga ggaccttccg ccccgcatcg
      961 agcaagagcg cggttgtcat gcgaaaccac aatccgctcc tcgctcaggt cgcgggttgc
     1021 gacggtctca agaccggcta catcgccgcg gctggattct cgatcgccgt caccgcgcag
     1081 cgtcaagacc gccgcctggt cgcggtggtc ctgggcagtg ccgatcggaa acggcgcgac
     1141 gcgaaagcag tcgagctgat cgagcgcggt tttgccaatt agcgcggctc cgtctcaaac
     1201 cgagggagct tgtcgccagt tcgtgtgcga tgggttttgg ccatggccac cgacggccga
     1261 cgcctaagac ttttgcttgt ttgtcggtcc gagcctggac tacgctggca tccggttcgg
     1321 gcaatcgagg tcacctgatg aacgcaacgg tctgtgccgg gatcgaggtt tggagcatgc
     1381 ggacgcgcct ctgcgagttc acgctgggtc tggcggttat cgcgtcggcg gtgggtctcg
     1441 acctgttttg tgcgcttggc caaccggcac ccggtgaatc cgccccccgc agcaagttta
     1501 ctctggcctg tcgtctggcg aactatcagg gatgcgagca ggctgcgtgg acgcatctgc
     1561 cagccattgg agttcggcat gtcttcatga gcgtgcccgc tgccgatcag gttgaaatcg
     1621 ttcggaaacg ccttgccgat catggtttga aggcggtggt gtttcggggc gatgccgatc
     1681 ttgcgcgtcc ggagggagtc gatcaactcg acgggcaact ggctgtctgc gagcgcatgg
     1741 gggtcaagta cctgttcctg tcggccaaga gccgcgatgt ccccaaggcg ctcgtttacc
     1801 agcgattgcg gcgggcgggc gacctggccg cccggcatgg ggtgacgatc gcgatcgaga
     1861 cgcaccccga tcttgggaca aacggggacg tgctcctcga gaccatgcgg caggtcgatc
     1921 atccgtgcgt gcgcgtcaac ttcgacaccg gcaacatcca cttttacaac gatggaatgg
     1981 acgctccgac tgaactgcgc aagattctgc cctttgtggc caccgtcgag atcaaggacc
     2041 atgacggcgg ggtgggggag tggcgttttc ccgccctggg ccgagggcgc gtcaacatag
     2101 ccgccgtgct tcagattctt actgaacacg gctacagcgg tccgatcacg atggagatcg
     2161 agggagtcaa ggatgtctcc cgcactccgc agcagattca agaggacatc gcccagtccg
     2221 cggcctatct gcgaacgctg gcggcgttcg aatgaagcga tagcaccgat acgatcccgg
     2281 acataacacc atgatcacac ctgttgcctc ccgcactctc cgactcgccc tgttcacgct
     2341 ggccctcagc ctctgtgccg gctcgaagac aatggccggc accgccggcc agcgcgcctc
     2401 agggcgtctg atcgcgcgca tccatgactc cggattcccc cagggccacg atacctacaa
     2461 cgggatgctg gcggccagtg acgggcgcat ctattacgtg ctgtgctcgg aggcgtacat
     2521 ctggcctgcc agatgtacgc gtacgatccc aagaagg
//
LOCUS       DAOQRO010000412         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_49058_length_2542_cov_5.845597, whole genome shotgun sequence.
ACCESSION   DAOQRO010000412 DAOQRO010000000
VERSION     DAOQRO010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49058_length_2542_cov_5.845597"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..690
                     /locus_tag="PKK20_12480"
     CDS             16..690
                     /locus_tag="PKK20_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNV00747.1"
                     /translation="MRKTILRWRANFFTGLLVVMPVVASLAIVLWLFSAVSGVTNSLL
                     FFLPKTLTHAGNGQGQMSWYWSVLALLISFLLVALVGRATRNYVGRQLIRGVDRLMSS
                     VPLLNKIYGTLKQVNEAFTSTQKSSFKQVVLIEYPRKGIHSIGFVTNDEIPEASVRLG
                     QTMVGVFVPTTPNPTSGFLMVVPHDQIIRLDMTVTDAFKYVFSLGSIAPELRAPSVVE
                     SRQIPE"
     gene            687..1373
                     /gene="recO"
                     /locus_tag="PKK20_12485"
     CDS             687..1373
                     /gene="recO"
                     /locus_tag="PKK20_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNV00748.1"
                     /translation="MTIERATGLVLRTRPLTETSLIVHWITPDYGRLATVAKGARRPT
                     SPFRGKLDIFYLAEFSFQRSRRSDLHTLREVGLRQTHPHLREDYARLRQAAYGALLVE
                     QMTEADTPLPVIYDLLVQLVDCLDSDGARPQYVLAFELKLLAEGGQAPDWGAARLPSA
                     SRDLARQLAAIELHEAARLDPPSKALAEVGTFLERFLVYHLDRVPRGRDEAIAARSPT
                     ASPTDARPGR"
     gene            complement(1336..>2542)
                     /locus_tag="PKK20_12490"
     CDS             complement(1336..>2542)
                     /locus_tag="PKK20_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNV00749.1"
                     /translation="HMLPPGDLRSLLVDGVIAGVGAVVVFLPQICLLFLFIGLLEDTG
                     YMARAAFIMDRLMNKVGLHGKSFIPLLSSFACAIPGIMATRTIENARDRLVTILIAPL
                     MTCSARLPVYTVLISACIPDTRALGFIKWPGLTLLSMYALGTVAALAAAWIFKRTLLK
                     GRTPLLMMELPPYRRPMLTAVLRHMWDRAALFLQQAGTVILGINILLWFLASYPRSAD
                     LERPYAEARRTLEARLAESGSEPEHSALREQIERLGVEQAGARLRNSVAGHMGRWIEP
                     IIRPLGFDWKIGVGIITSFAAREVFVSTMSVVYNVSRGEDTEGDSLALTLRRQVRPDG
                     RPQYTMLMGISLMVFYVFALQCASTVVVVRRETNSWKWPLFQWAYLGALAWMAAFLTF
                     QGGRLLGLQ"
BASE COUNT          481 a          853 c          752 g          456 t
ORIGIN      
        1 ggctagactc acgccatgcg caagacaatc ctgcgttggc gggccaattt cttcactggt
       61 ctgctggtgg tgatgcccgt ggtggcgtca ctcgccattg tcctctggct cttcagcgcg
      121 gtctccgggg tgacaaactc gctgctcttt ttcttgccca agaccctgac ccacgcggga
      181 aacggacaag gacagatgag ctggtactgg agtgtcctcg ccctcctcat tagcttcctt
      241 ttggtggcgc ttgtgggtcg ggccacccgg aattacgtcg gacggcagct cattcggggt
      301 gtcgaccgtt tgatgtcgag cgtcccgctg ctcaacaaga tctatgggac gctcaaacag
      361 gtcaacgagg ccttcacctc gacccagaaa tcctcgttca agcaggtcgt gctcatcgag
      421 tacccgcgca agggcatcca ttccatcggt tttgtgacaa acgacgagat tcccgaagcc
      481 tcggtccggc tgggccagac catggtgggc gtgtttgttc ccacaacgcc caatcccaca
      541 agcggattcc tcatggtggt gccccacgat cagatcatcc ggctggatat gaccgtgacc
      601 gacgccttca agtacgtctt cagcctcgga tccatcgcgc cggaattgcg cgctccgtcc
      661 gttgtggaga gccgccagat cccagaatga ccatcgagcg cgccacgggc ctcgtcctcc
      721 gcacccggcc cctgaccgag acaagtctga tcgtgcactg gatcaccccg gattacggtc
      781 gcctggccac ggtcgccaag ggcgcacgca gacccacctc gccattccgc ggcaaattgg
      841 acatctttta tctcgctgag ttctcgtttc agcgcagccg ccgctccgat ctgcatacgc
      901 ttcgcgaggt cggcctgcgc caaactcatc cccatctccg cgaagactac gccaggctcc
      961 ggcaggcagc ttacggagcc ctgcttgtcg agcagatgac cgaggccgac acgcccttgc
     1021 cggtcattta cgatctgctg gtccaactgg tggactgcct cgattccgac ggcgcccggc
     1081 cccagtacgt gctggccttc gaactcaagc tgctcgccga gggcggccaa gctcccgact
     1141 ggggggccgc tcgacttcct tcagccagcc gcgatctggc tcgacaactc gcagcaatcg
     1201 aactgcacga ggccgcgcgg ctcgatccgc cgtcgaaagc gctggctgaa gtgggaacat
     1261 ttctcgagcg attccttgtt taccatctgg atcgcgttcc gcgggggcgc gacgaggcga
     1321 ttgcagcgcg ttcgcctact gcaagcccaa cagacgcccg ccctggaagg taaggaatgc
     1381 tgccatccac gccaacgccc cgagataggc ccactggaac aacggccact tccaactgtt
     1441 ggtctcgcgc cgaacgacca cgacggtgct ggcgcactgc agcgcgaaga catagaagac
     1501 catcagcgaa atccccatca gcatcgtgta ctggggccgg ccgtccgggc gaacctggcg
     1561 gcgcagcgtc agcgccaggc tatcgccctc ggtatcctcg ccgcggctca cgttgtatac
     1621 aaccgacatt gtgctcacga agacctcgcg cgcggcaaac gaggtgatta tcccgacgcc
     1681 gatcttccaa tcgaatccca gcgggcgaat gatcggttcg atccatcggc ccatgtgtcc
     1741 ggcaacactg ttgcgcagcc gggcgccggc ttgctccacg cccagccgct cgatctgttc
     1801 gcgaagggcg ctgtgctccg gttccgatcc gctctcggcc agacgggctt cgagtgtgcg
     1861 gcgcgcctcc gcgtatggcc tctcgaggtc ggcgctgcgc gggtaactgg caaggaacca
     1921 gagcagaatg ttgattccca aaatgaccgt ccccgcctgt tgcagaaaga gcgcggcgcg
     1981 gtcccacatg tggcgcagaa ctgccgtcag catgggacgc ctgtagggcg gcaactccat
     2041 cataagcaac ggcgtgcggc ccttgagcag ggtccgcttg aagatccagg ccgcggccag
     2101 cgccgccacg gtcccgagcg catacatgct caagagagtc agccccggcc acttgatgaa
     2161 gcccagcgcc cgcgtgtcgg gaatgcaggc ggagatcagc accgtgtaga ccggcagccg
     2221 cgccgagcaa gtcatcagcg gggcgatcag gatcgtcacc agccggtccc gcgcattctc
     2281 gatcgtccgc gtcgccatga tgccggggat ggcacaggcg aaggaactca gaagcgggat
     2341 gaagctcttg ccgtgcaggc ccaccttgtt catgagcctg tccatgatga aggccgcccg
     2401 ggccatgtag cccgtgtcct cgagcaggcc gataaacaga aacagcaggc agatttgcgg
     2461 cagaaagacc accaccgccc ccacaccggc gatcacccca tccaccaaaa ggctgcggag
     2521 gtcgccgggg ggcagcatgt gg
//
LOCUS       DAOQRO010000413         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_49229_length_2537_cov_5.070105, whole genome shotgun sequence.
ACCESSION   DAOQRO010000413 DAOQRO010000000
VERSION     DAOQRO010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49229_length_2537_cov_5.070105"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKK20_12495"
     CDS             <1..1066
                     /locus_tag="PKK20_12495"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNV00750.1"
                     /translation="LEALRLGKPVVTANKALLSARGEELFAAAGRSGANIYYEASVAG
                     GIPIIKSLREGFIGNRILRLYGIVNGTCNYILTRMKLDGAEFADVLAEAQRLGYAEAE
                     PSLDIDGHDSMHKVGILASLAHGFWVKPRDIHVEGIRNVSQMDIQFAGQLGYTIKLLG
                     VVKQMHPPAGRGRGRAEAGPVLVAVYPALVPNSHVLASVNHAFNAVFVQGDVVGDTLF
                     YGRGAGQDPTASAVLSDLADAALDLTHESRRRVPPFVAHAKNGRVLALSEAVCRYYVR
                     LRVVDRPGVLGRVAAILGRNRIGISSVFQPEGHEGGVVPLILMIHEAPNRAMARALAQ
                     IGRLAAVKAPPVMFRVESFK"
     gene            1096..2487
                     /gene="thrC"
                     /locus_tag="PKK20_12500"
     CDS             1096..2487
                     /gene="thrC"
                     /locus_tag="PKK20_12500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNV00751.1"
                     /translation="MEPVVLESTNEQSPPVGLREALLRGQAPDRGLYWPRRFPSLSSE
                     EIAAFAGLPYWRIAWEVLRRYMSGVLDAEALEAVCRDAYDFAVPLEPIGDRIHVMRLD
                     RGPSASFKDFAARWMARVIGRFVREEGGELTILTATSGDTGSAVASAFHDVPGIRVIV
                     LFPVGEVSERQRRQMTILRGNIRAVAVGGKFDDCQAMVKRAFSDPALDAIPLTSANSI
                     NIGRLLPQSVYYFYAASRVAECGEPVVFAVPSGNFGDMMGAVVARKMGLALKRLVAPV
                     NGNDAFPRFLASGTYGKIEPSRACLSNAMNVGHPSNLARLVAAYGGRMDEAGAIHRQP
                     DLDRMRRDLYSTSVSDARTREAIRAAWERHRLLLEPHGAVGWRGLEDYLAVEPLGGSP
                     AVVLETAHPAKFPDEIVALLGFAPDVPPALAALEQCPEDYDRLEVDYESFRNYLLERH
                     SMGSAGRQSSTRS"
BASE COUNT          381 a          720 c          965 g          471 t
ORIGIN      
        1 cctcgaggcc ctgcggttgg gcaagccggt cgtgacggcg aacaaggcgc tgctttcggc
       61 gcggggggag gagttgttcg ccgcggcggg gcgatcgggc gccaacatct attacgaagc
      121 cagcgtggcc ggcggcattc cgatcatcaa gtcgctgcgc gagggtttca tcggcaatcg
      181 catcttgcgc ctttacggca tcgtcaatgg cacgtgcaat tacatcctga ctcgcatgaa
      241 gctcgatggg gccgagtttg cggatgtgct ggccgaggcg cagcggctgg ggtacgcgga
      301 ggcggagccc tcgctggaca ttgatgggca cgactcgatg cacaaggtgg gcatcctggc
      361 gtcgttggcg cacgggttct gggtcaagcc aagagacata cacgtcgagg ggattcgcaa
      421 cgtgtcgcag atggacatcc agtttgccgg ccagttggga tacacgatca agcttttggg
      481 tgtggtcaag cagatgcatc cgccggccgg ccgggggcgg gggcgggcgg aggccgggcc
      541 ggtgctggtg gcggtgtacc cggcgctggt gccgaacagc catgttctgg ccagcgtgaa
      601 tcacgcgttt aacgccgtgt ttgtgcaggg cgacgtggtt ggcgacacgc tgttctacgg
      661 acgcggggcg ggccaggacc ccacggcgag tgcggtgctg agcgatctgg ccgatgcggc
      721 gctggatctg acgcacgaga gccggcgccg agtcccgccc ttcgtggccc acgccaagaa
      781 cgggcgggtg ctggccctgt ccgaggcggt ctgccgctat tacgtgcggt tgcgggtggt
      841 ggatcggccc ggcgtgctgg gccgtgtggc ggcgatcctg gggcgaaacc gaatcggcat
      901 ttcgtcggtg tttcagcccg aaggccacga gggcggtgtt gtgccgttga ttttgatgat
      961 ccatgaggcc ccgaaccggg cgatggcgag ggctctggcg cagatcggac ggctcgcggc
     1021 cgtgaaggcg ccgccggtca tgttccgggt cgagagtttc aagtgagcgc atgtgtccga
     1081 cgcgggggtg acacgatgga accggttgtt ctcgaaagca ccaacgagca gtcgccgccg
     1141 gttggcctgc gcgaggcgct gctccgtggg caggcgccgg accgcggttt gtactggccg
     1201 aggcggtttc catcgttgag ctcggaggag attgcagcgt tcgccggcct gccgtattgg
     1261 cgaatcgcct gggaggtgct gcggcgctac atgagcggcg tgctcgacgc ggaggcgctc
     1321 gaggccgtgt gccgcgacgc gtacgatttt gctgttccgc tcgaaccgat aggcgaccgc
     1381 atccatgtga tgcgcctgga ccgcggcccg agcgcgtcgt tcaaggattt cgccgcccgc
     1441 tggatggcgc gcgtgatcgg gaggttcgtt cgggaggagg ggggcgagtt gacgatattg
     1501 acggcgacga gcggggatac gggatccgcg gttgcgagcg cttttcatga cgtccccgga
     1561 atccgtgtga ttgtgctgtt tcccgtcggt gaagtcagcg agcgccagcg ccggcagatg
     1621 accatcctgc gcgggaacat ccgcgcggtg gccgttgggg ggaagttcga cgattgccag
     1681 gcgatggtga agcgggcgtt ctcggatccg gcgctcgatg cgattccgct gacatcggcc
     1741 aactcgatca acatcgggcg gcttctgccc cagagcgtat actatttcta tgcggcgtcg
     1801 cgcgtggccg agtgcgggga gcctgtggtg ttcgcggtgc ccagcgggaa ttttggggac
     1861 atgatgggcg cggttgtcgc ccgtaagatg gggcttgcgc tcaagcgcct ggtggcaccg
     1921 gtcaatggca acgatgcgtt tccgaggttc ctggcgagcg ggacctacgg gaagatcgaa
     1981 ccgtcgcgcg cctgcctctc caacgccatg aatgtcgggc atccgagcaa cctggcgcgc
     2041 ctggtggcag cctacggggg gcgcatggac gaggcgggcg cgatccatcg ccagcctgat
     2101 ctggaccgga tgcggcgcga cctctactcg acctcggttt cagacgcgcg gacgcgggag
     2161 gcgattcgcg cggcgtggga gcggcatcgt ctgctcttgg aaccgcacgg ggcagtcgga
     2221 tggcgcgggc tcgaggatta cctggctgtc gagccgttgg gcgggtcgcc ggcggtggtg
     2281 ctcgagacgg cgcatccggc gaagttcccg gatgagattg tggcgttgtt gggttttgcg
     2341 ccggacgtgc ctcccgcgct ggccgctttg gagcagtgcc cggaggatta cgaccggctc
     2401 gaagtggact acgagagctt ccggaactac ctgctcgaaa gacactcgat gggctcggcc
     2461 gggcgccagt cgagcactcg gagttgaacc gtgcggcgcc cgtcgtacga gttcacctgg
     2521 ggcgtgcagg ccaggtc
//
LOCUS       DAOQRO010000414         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_50364_length_2502_cov_3.820188, whole genome shotgun sequence.
ACCESSION   DAOQRO010000414 DAOQRO010000000
VERSION     DAOQRO010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50364_length_2502_cov_3.820188"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2502)
                     /locus_tag="PKK20_12505"
     CDS             complement(<1..>2502)
                     /locus_tag="PKK20_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNV00752.1"
                     /translation="KQVPIVEKTSLGLVQTLRVTLDHPYAEAADAVVRLGAEQPNALR
                     LQPGRQTIEWRVPAVASAETAPLVLEVAGQTVVQTELRRAPVRPLTVYVLPHSHTDIG
                     YTEIQTEIEDKQVQNLVRGMEIARQTASYPPGARFVWNVEVLWAADLYLRRLGETQRK
                     EFIEAVRAGRVALNGLYLNELTGLCRPEELIRLFRMATRLGTETGVPVDAAMISDVPG
                     YSWGTVSAMTEAGIRYFSVAPNYFDRIGDILVQWENKPFYWVGPDGRSKVLVWIPFWG
                     YAMSHRYHAMSPRLVEDFCNGLAQRAYPYEIAYVRWSGVGDNATPDPAICEFIKDWNQ
                     THASPQFIISSTGTAFRAFEERHGAELEQVAGDWTPYWEDGAGSSALETAMNRASSDR
                     VIQAEALWAMLNPRTYPVDAFEDAWRNVLLYSEHTWGAWCSVSEPFRRETREQWAVKQ
                     SYAVAADLQSRDLLSRALAQSDGPAQNAAVDIFNTTSWPRTELVVVPKFLSEGKDRVT
                     DDSDQPVASQRLRSGELVLLARDVPPFAARRYRLASGAPHVDGKVTARGATLENGIVS
                     LCVDERTGGIVELRLAGLDANLADAADGQALNEYLYFNGDKPSNARRNGPVTIRERET
                     GPLVASLLIASDAPGCHRLEREIRLVAGRDAVEIVNTLDKSRLLASSYHAAEGKESLN
                     FGFPFAVPDGQMRLEVSYGVIRPDLDQIPSACKNWFSVNRWADVANDRFGVTWITLDA
                     PLVQVGELSANLLNSQANPEVWRKKVGPTQKLYSWAMNNHWGTNYRACQDGPHVFRFV
                     LRPHCGYDPLAASRLAIAASQPLLPTVARGSKPIG"
BASE COUNT          443 a          854 c          781 g          424 t
ORIGIN      
        1 cgccgatggg tttagacccg cgcgccacag tcgggagcaa cggctggctc gcggcgatcg
       61 cgagcctcga cgcagccaac ggatcgtaac cgcagtgcgg gcggaggacg aaccggaaca
      121 cgtgcggccc gtcctggcag gcccgatagt tcgtccccca atggttgttc atggcccacg
      181 agtagagttt ctgagtcgga ccgaccttct tccgccacac ctcggggttg gcctgcgagt
      241 taagcaggtt cgccgacagt tcgccgacct gcaccagcgg cgcatcgagc gtgatccagg
      301 tgacgccgaa ccgatcgttc gccacgtccg cccaacggtt cacgctgaac cagttcttgc
      361 aggcgcttgg aatctgatcg agatcggggc ggatgacgcc ataagacacc tcgaggcgca
      421 tctggccatc gggcactgca aaggggaagc cgaaattaag actctccttg ccctcagccg
      481 cgtggtagct cgatgcgagg agacggctct tgtccagcgt gttgacaatc tcgacggcgt
      541 ctctgccggc aacgagccgg atttcgcgtt cgaggcgatg gcaacccggc gcatcggacg
      601 cgatcaggag cgatgcgaca agcggacccg tctcgcgctc gcggatcgtg accggcccgt
      661 tgcgcctggc attggacggc ttgtccccgt tgaaatagag atactcgttg agcgcctgcc
      721 catcggcggc gtcggcaaga ttggcatcga gacccgccag acgcagctcg acgatcccgc
      781 cggtccgctc gtccacacac aggctgacga tcccgttctc cagcgttgcg cctcgcgcag
      841 tgacctttcc atcgacgtgc ggggcgcctg aggcgagcct gtaacgccgc gccgcaaacg
      901 gtggcacgtc tcgggcgaga aggaccagtt cgccgctgcg caaccgctgc gacgccacgg
      961 gctggtccga gtcgtccgtg acgcggtctt tgccctccga aaggaacttg ggcaccacga
     1021 ccagttccgt gcgcggccat gatgtggtat tgaagatgtc cacagccgcg ttttgggccg
     1081 gaccgtcgga ttgggcgagg gcacggctaa ggaggtcgcg cgactggaga tcggcggcga
     1141 cggcatagga ttgcttgacg gcccattgct cgcgggtctc gcggcggaag ggttcgctga
     1201 cgctgcacca ggcgccccat gtgtgttcgg agtagagcaa cacgttgcgc caggcatcct
     1261 cgaacgcgtc aaccggatac gtccgcggat tgagcatcgc ccagagcgcc tcggcctgga
     1321 tcacacggtc cgaactcgcc cgattcatcg ccgtctcgag tgccgacgac ccggcgccat
     1381 cctcccagta gggcgtccag tcgccggcca cctgctcgag ctccgccccg tgccgctcct
     1441 cgaaggcgcg gaaggccgtg ccggtcgagc tgatgatgaa ttggggcgaa gcatgcgtct
     1501 ggttccaatc cttgatgaac tcgcagatcg cagggtcggg cgtggcgttg tcccccacgc
     1561 cgctccaccg cacgtaggcg atctcatagg gatacgcccg ctgcgccagg ccattgcaga
     1621 aatcctcgac caggcgcggc gacatcgcgt ggtaccggtg ggacatcgcg taaccccaga
     1681 acggaatcca gaccagcacc ttgctccgcc cgtccggccc gacccagtaa aacggcttgt
     1741 tctcccactg caccaggatg tccccgatgc gatcgaaata gttcggcgcc acgctgaaat
     1801 accggatgcc ggcttcggtc attgcgctca cggtccccca cgaatagccc ggcacgtcgc
     1861 tgatcatcgc cgcgtccacg ggcacgcccg tctcggtccc caggcgcgtc gccatgcgaa
     1921 agagccggat caactcctcc ggtcggcaaa gaccggtgag ttcgttcaga tacaggccgt
     1981 tgagagcgac ccgcccagcc cgcacggcct cgatgaattc cttgcgctgg gtctcgccaa
     2041 gccgccgcag gtacaggtcc gccgcccaca gcacttcgac attccacaca aaacgcgccc
     2101 ccggcggata gctcgccgtc tggcgggcga tctccatgcc gcgaaccagg ttctgcacct
     2161 gcttgtcctc gatctcggtc tggatctccg tgtagccaat gtcggtgtgc gaatgcggca
     2221 gcacataaac cgtcaacggg cgcacaggcg cccgacgcaa ctcggtctgg accacggtct
     2281 gacccgccac ttcgagaacc aggggcgccg tctccgcgct cgccacagcc ggcacacgcc
     2341 actcgatggt ctgtcgcccg ggttgcagac gcagggcatt cggctgctcc gcgccgagcc
     2401 gcaccacggc gtccgcagcc tccgcatacg ggtgatctaa cgtgacgcgc aacgtctgga
     2461 ccaggccgag gctggtcttc tcgacaatcg ggacctgttt ga
//
LOCUS       DAOQRO010000415         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_50573_length_2495_cov_5.782377, whole genome shotgun sequence.
ACCESSION   DAOQRO010000415 DAOQRO010000000
VERSION     DAOQRO010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50573_length_2495_cov_5.782377"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            663..>2495
                     /locus_tag="PKK20_12510"
     CDS             663..>2495
                     /locus_tag="PKK20_12510"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HNV00753.1"
                     /translation="MNLRVLWLGTMIVLPALSGTPAPSQVITFDARSYLHTDVRDPAQ
                     ARRAWDALHLLAALQGLANRDAPRFYVLFAQGFQVATDQFWLDWFRKEDGWLRTTQLE
                     PVADIDGALAAMRSHYTGLVVYDPNVPATACVASTAAGCERLLPVRYDPSPDSLFTRC
                     VRTLNLPIGLWLVHPDGSSKFTGTGQIPDSTKPSSGSAKIDAYRWAAARYLQTGRCDP
                     HFAAYYMDADWLRRPGNAPADNHTLPNHDYFIAGRAFFFDLSPWGDEAPVDDPGQSVG
                     LDRRMLLDLLRAFHDGTSAGITKIGGFPPWPFKYTTHGGAGRHKPVPTEWEFTRLISQ
                     FNAYHEADAAGHGAMANASFFAHYPLTPPCRQPNPIPSRAQWQVRGYVTSDGRVASRF
                     FVGHYVGDYDAPSWLYKAVPAFFRDPDRGRVPLGWAFDPNLADRAPQALVYAYRHATT
                     NDFFIAGDSGAGYLNPRALTVRPDSGLPSGLAAWEAHCQRAYKDWGMTITGFALDGSA
                     GASTSTEFLAYKSFSPDGIGTHFEPQPRLIAGIPTCPEKDLPDGAAEAAALIARRAEA
                     RGAGPGFLWARSILKPPRWYAEVSALLRERHPHLPRVVVLRGELR"
BASE COUNT          407 a          811 c          788 g          489 t
ORIGIN      
        1 gggtctccac acagattcag gtcggaacac ggatggagaa ggagagaggc ggtgtaaagt
       61 ggtttgccgg cgggttcgag atggcgggtc gcggttccgt tgtccggggg gacatcgcac
      121 cggatgcgaa tgggctgagc gcggtgtcag gtcgtaggcg acactgcaga cggccatctg
      181 tgttccgccc tgaatcggtg tggggactct ctgtgcatag gatcagtgag ggtctccaca
      241 cagattcagg tcggaacacg gatggagagg gagagaggcg gtgtaaagtg gtttgccggc
      301 gggttcgaga tggctggtcg cggttccgtt gtccgggggt acatcgcacc ggatgcgaat
      361 gggctgagcg cggtgtcagg ttgtagccga cactgcagac ggccatctgt gttccgccct
      421 gaatcggtgt ggggactctc tgtgcatagg atcagtgagg gtctccacac agattcaggt
      481 cggaacacgg atggagaagg agagaggcgg tgtgtcggcc ccccgcgggt gtgttctgag
      541 gatcgagtcc gttgcatttc cttgtggccg gttccacctg gtccgatgct ggtcgcctcg
      601 gcatcagatt cccggactgg gccggcttgc caccgcgcca tccagaaggc acgatcccgc
      661 ggatgaacct ccgcgtgttg tggctcggaa cgatgattgt cctgccagcg ctctcaggaa
      721 ctccggcacc ctcgcaggtc ataacattcg acgcgcggtc ctatctgcac accgatgtcc
      781 gagatccggc gcaggcccgc cgcgcgtggg atgcccttca tcttctcgcc gccctgcagg
      841 gtctggccaa tcgcgacgcg ccacgtttct acgtcctctt cgcgcagggc tttcaagttg
      901 ctacagatca attctggctt gactggtttc gcaaggagga cggctggctg agaaccactc
      961 aactcgaacc cgttgccgac atcgatggtg cgctggccgc catgagatcg cactacaccg
     1021 ggctggtggt ctacgatccg aatgtgccgg ccaccgcatg cgttgcgtcg accgcggccg
     1081 ggtgcgagcg cctcctgccg gtgcgttacg atccgtcacc cgactcgctc tttacccgct
     1141 gcgtccgcac attgaatctg ccgattggcc tctggctggt ccatcccgac ggctcgagca
     1201 agttcaccgg cacgggtcag atcccggact cgaccaagcc gtcatcggga agcgcgaaga
     1261 tcgacgcgta ccgctgggcc gcggcgcgtt acctccagac gggccgatgc gatccgcact
     1321 ttgcggcgta ttacatggat gccgactggc tgcggcgccc cggcaatgcg cccgcggaca
     1381 accacaccct gcccaaccac gactatttca ttgccggtcg cgccttcttc ttcgatctct
     1441 cgccgtgggg cgacgaggcc cctgttgatg atcccggaca atcggtgggg cttgatcgcc
     1501 ggatgcttct tgacctcctg cgcgcattcc acgacggcac ctcagccggc atcaccaaga
     1561 tcggcggctt tcctccctgg cctttcaagt acacgaccca cggcggagcc ggccgacaca
     1621 aacccgtccc caccgaatgg gagttcacgc gattgatttc ccagttcaac gcctaccatg
     1681 aggccgacgc cgccggccac ggcgcgatgg cgaatgcatc tttctttgcg cattacccgc
     1741 tgacccctcc gtgccgccag cccaatccca ttccatcacg cgcccagtgg caggttcgag
     1801 gctatgtgac ctccgacggg cgggtcgcgt cgaggttttt cgtcgggcac tacgtcggcg
     1861 actacgatgc cccatcgtgg ctctacaaag ccgtgcccgc attttttcga gaccccgatc
     1921 gagggcgggt gcccctgggc tgggctttcg atcccaatct ggccgatcgc gcgccgcagg
     1981 cgttggtcta tgcctatcgc catgccacaa ccaatgactt cttcatcgca ggcgactccg
     2041 gcgccgggta tctcaaccca cgcgccctca cggtgcgtcc cgactccggt ttgccctcgg
     2101 gtctggccgc ctgggaagcg cactgccaac gcgcctacaa agactgggga atgaccatca
     2161 ccggtttcgc gctcgatgga tcggccggcg cgtccacctc gacggagttc ctggcttaca
     2221 aatccttcag ccccgatggc atcggcactc atttcgagcc tcagccgcgc ctgatcgccg
     2281 gaatcccgac ctgtccggag aaggatcttc cggacggcgc ggccgaagcc gccgccctga
     2341 tcgcccggcg cgcggaagct cgcggtgcgg gtccgggatt cctctgggcg cggagcattc
     2401 tgaaaccgcc gcggtggtat gctgaggtgt cggcgctgct acgggagcgg catccgcacc
     2461 tgcctcgggt agtcgtcctg cgcggcgaac tgcgg
//
LOCUS       DAOQRO010000416         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_50940_length_2484_cov_5.224784, whole genome shotgun sequence.
ACCESSION   DAOQRO010000416 DAOQRO010000000
VERSION     DAOQRO010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50940_length_2484_cov_5.224784"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="PKK20_12515"
     CDS             <1..1954
                     /locus_tag="PKK20_12515"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNV00754.1"
                     /translation="ARIHYTLDGSDPDETSPRHTEPLRIATRIGTPNGISMIPTVPGG
                     LPPAGEVFKAWVVRARALKPGALPSPTVTRTFWVDPRGRSRYSLPVVSLATDRANLFD
                     HAIGIYVPGDAPNGNYSQRGPEWERPAHVELYETDNQPAFAQEIDVKIHGNTSQGFPI
                     KGLDLDGTGGRGRQQFRYRLFPDRAREKLEHFLLRPAGHDQPTAFMRDELMQSLAAET
                     GAESQAARACVLFINGEYWGLHYLKEKEDAQFVAHYADRAEDAIDYLEGYASPKAGDT
                     LHYQAMIDFIATHDLADPAHYAHVATLMEVPNYIDYKACEIFFYRWDIGNHRLWRPRT
                     PDGRWRWLQFDNDVGWGGFWAEQPAWLFDMLAADLTPDGSLHGHNNEITTFLLRHLMR
                     NAQFRRDFVNRFQDLLNTLFLPSHTQARIDQMASVLAPEMAEHTRRWRSPGSLADWQN
                     NVHYLRMYARNRPAAMREHLRQYFRLDPSCTFTGGVRPSQAGALRLNSLELREPADQP
                     WSGLYFQRHPITVRAEPRPGFRFDRWDGLPGVTTNEVTLLLNGDFALTAQFAPDRATQ
                     PRFARIRRSGAGSVELTVAGAPHASLQLESSEDLSAWSTRQSLELDDAGYAVITEPLA
                     TPPRHQFYRLRSSDGTSGCCGCSVAR"
     gene            complement(2179..>2484)
                     /locus_tag="PKK20_12520"
     CDS             complement(2179..>2484)
                     /locus_tag="PKK20_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV00755.1"
                     /translation="TVIRFDNQSSDCRTVIEIEAEDRMGLLYTIAQVLAELDVDISVA
                     KISTERGVAMDTFYIAERDGSRVEAPARLGEIQERLRAAIDSHATVHGSPVYGLCKN"
BASE COUNT          451 a          912 c          693 g          428 t
ORIGIN      
        1 cgcgcgcatc cattacacgc tcgatgggtc cgatccggac gaaacatcgc cccgccacac
       61 cgagcccctg cggatcgcaa cacgcatcgg cactcccaac ggcatctcga tgatcccgac
      121 cgtgcccgga gggctgccgc ccgccggcga agtcttcaag gcctgggtcg tccgggcccg
      181 cgcgctcaaa cccggcgcgc tgcccagccc gaccgtcacc cgcaccttct gggtcgatcc
      241 gcgcggacga tcccgatact cgttgccggt ggtgtccctg gccacagacc gcgcgaacct
      301 gttcgaccac gccatcggca tttacgtgcc cggcgatgct cccaacggca actactccca
      361 acgcggcccc gagtgggaac gcccggccca tgtcgagctc tacgagaccg acaatcagcc
      421 ggccttcgcc caggaaatcg acgtcaagat ccacggcaac acctcgcagg gattcccaat
      481 caagggactt gacctcgacg gcactggcgg acgcggacgg cagcagttcc gctatcggct
      541 cttccccgat cgcgcccgcg agaaactcga gcactttctc cttcggcccg ccgggcatga
      601 ccagcccacc gcattcatgc gcgacgagct gatgcaaagc ctcgcggccg agacgggcgc
      661 cgaatcccag gccgcccgcg cctgcgtcct gttcatcaac ggcgagtact ggggtctgca
      721 ttacctgaag gaaaaggaag atgcccagtt cgtcgcgcac tacgccgacc gcgccgagga
      781 cgccatcgac tacctcgagg ggtacgcctc gcccaaggct ggggacacgt tgcattacca
      841 ggccatgatc gatttcattg caactcacga cctggccgat cccgcgcact acgcccacgt
      901 cgccaccctg atggaagtgc ccaactacat cgattataag gcctgcgaga ttttctttta
      961 ccgatgggac atcgggaacc accgcctgtg gcgcccacgc acgccggacg gacgctggcg
     1021 ctggctccag ttcgataacg acgtcggctg gggcggattc tgggccgagc agcccgcctg
     1081 gctgttcgac atgctggccg ccgatctcac cccggacgga tccctccacg gccacaacaa
     1141 cgaaatcacc actttcctcc tccgccactt gatgcgaaac gcgcagttcc gacgcgactt
     1201 tgtcaaccgc tttcaggacc tcctgaacac gctcttcctc ccctcccaca cccaggcgcg
     1261 catcgaccag atggcgtccg tgctggcgcc cgaaatggcc gaacacacgc gccggtggcg
     1321 ctcgcccggc tcgctggccg attggcaaaa caacgtccac tacctccgca tgtacgcccg
     1381 caatcgcccc gcggccatgc gcgaacacct gcgccaatac ttccgactcg atccgtcctg
     1441 cacgttcacg ggcggcgtcc gcccatccca ggccggcgca ctccgcctca actcactcga
     1501 gctccgggag ccggcagacc agccctggtc cggcctctac ttccaaagac atccgatcac
     1561 cgtgcgcgcc gagccgcggc cgggctttcg attcgaccgc tgggacggcc tgccgggtgt
     1621 gaccaccaac gaggtcacgc tgctcctgaa cggcgatttt gcgcttacag cccagttcgc
     1681 tcccgaccgt gcgacacaac cgcgctttgc acgcattcgg cgctccggcg ccggcagcgt
     1741 cgaactgacg gtggccggcg cgccccacgc cagcctccaa ctcgaatcct ccgaagactt
     1801 gagcgcctgg tccacacgcc aatcgctcga actcgatgac gccggatacg ccgtcatcac
     1861 cgaaccgctc gcaacgcccc cccgacacca attctaccgc ctgcgcagtt cggacggtac
     1921 ttccggttgt tgtgggtgtt ctgtcgcccg gtgaggagca tcgggcctgc aacggatgga
     1981 gattgccgga ggggcgatgt cctcgtcgcc cgctggagtt tggacattct cgatgcttgt
     2041 cgatgtggcg gcaggtcctt gatcgcacac ttcatcgttc tgcaggctct gttccgtcag
     2101 ttcgagccca aaccgcacag catcgttgcg attcggtccg gcagcgccat ggcgcacgct
     2161 tgctgaggag caacgaaatt agtttttgca caaaccataa accgggcttc catgaaccgt
     2221 ggcatgactg tcgatggcag cgcgcaggcg ctcttggatt tccccaagtc gcgccggcgc
     2281 ctcgacccgc gagccgtcac gctccgcgat atagaaggtg tccatggcca cgccgcgctc
     2341 ggtgctgatc ttcgccaccg aaatgtccac atccagttcc gccagcacct gggcgatcgt
     2401 gtagagaaga cccattcggt cctcggcctc gatctcgatg acggtccggc aatcgctcga
     2461 ttggttgtcg aagcggatga cagt
//
LOCUS       DAOQRO010000417         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_51963_length_2453_cov_4.140534, whole genome shotgun sequence.
ACCESSION   DAOQRO010000417 DAOQRO010000000
VERSION     DAOQRO010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51963_length_2453_cov_4.140534"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1919
                     /locus_tag="PKK20_12525"
     CDS             207..1919
                     /locus_tag="PKK20_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00756.1"
                     /translation="MKLHALLISLIACGGGCVVWPLNAHPAPTALAEASAQVEARLKS
                     PDWALSWHWVGTTRLAGNTNGTEARVILAQPASQRMLRTTLEKLARSPGAWLAPGAAT
                     NPPAASLLIPWIEELVASQSCGGIVFGAEDGVAIVAAVRVSNEKRNTLESTWLAWAGA
                     ADGTTPTEVQTENAKVRETRFAQTQGWSRVGCAGDWALIGMGTGSAAPFVALAKRVAG
                     LGAPEGWLEIEADGPKVAQILGWPEPASWPAIGIQIEGRGPILRTTASLTYSTPLDLK
                     LDPWRIPTHTLGDPVVGFTALRGIRPWLSRQAWWKQLELPTTPNQAVGWTIAEIPFAS
                     YVAWEAPEVTNVMTQLGPKVSAVLERYIPKVRFGDAMFQTNTTRLAWTNLPIAEPFVS
                     PATAQDPGFAVAGLFPLVKTKRPLPPELLSQIMDRIDLVYYSWELTAQRVNGWRHIKN
                     LYWMLAGYAPLPTNYTGEAWLFDTNVVDRLGNTGTELRLESPRHLVGVRNSALGLSGL
                     ELVALMRWLDDPDFPRLSEPAARPALLHRMRARQTGKPGAGVPPASPAPSNAPAAPPV
                     RPSP"
     gene            1927..>2453
                     /locus_tag="PKK20_12530"
     CDS             1927..>2453
                     /locus_tag="PKK20_12530"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNV00757.1"
                     /translation="MLKLGVNIDHVATLREARYRGLDAGEPDPVDAARRCEAAGAHGI
                     TAHLRKDRRHIQDRDVWRLRETIRTRLNLEMANSPPIVDLVLELKPEIVCLVPERRQE
                     VTTEGGLDVAGQCSALARTRRRLNKAGIEVSLFITPDLHQVAAAARVGSQFVELHTGR
                     FAKHFENLAGRKREL"
BASE COUNT          446 a          790 c          771 g          446 t
ORIGIN      
        1 gtgacaggtt cttaatttga cagtggtttg tcgagatcat cgcacgccta ctgcgcacca
       61 aaccacgcca attgataagc gctctttcca acctcaacaa cagcaaccct tcaggaattt
      121 aaatgaaata accgtgcccc ggcgcccgtt tccgcgcctt tccatttgct aatcggacag
      181 ttctttggca aggagttggc gtttctatga aactgcatgc tctgttgatc agcctgatcg
      241 cctgcggcgg cggctgtgtc gtctggccgc tgaacgcgca tccggcgccg acggccctcg
      301 ccgaggcatc agcccaagtc gaggctcggt tgaaatcgcc ggactgggcg ctctcctggc
      361 attgggtggg cacaacgcgc ctcgcaggca acacgaacgg cactgaggcg agggtgatcc
      421 tggctcagcc ggcctcgcaa cgcatgctgc gcaccacgct cgagaagctg gcccgatccc
      481 caggggcctg gctcgctccg ggcgctgcaa caaatccccc tgcggcatca ttgttaattc
      541 cgtggatcga ggagttggtc gcatcgcaaa gctgtggcgg gatcgtgttc ggcgctgaag
      601 acggcgtggc gatagttgcc gcagtgcggg tgtccaatga gaagaggaac accctcgagt
      661 caacgtggct tgcgtgggcg ggggcggcag acggcacaac gcccaccgag gttcagacag
      721 agaatgcgaa ggttcgggaa acccggtttg cccaaacgca aggctggtcc cgggtcggct
      781 gcgccgggga ctgggcattg atcggcatgg gcacaggctc ggccgcgccc tttgttgctc
      841 tcgctaagcg cgtcgccggt ctgggcgctc ccgagggttg gctcgagatc gaggcggacg
      901 ggccaaaagt ggcgcagatt ctgggctggc ccgagccggc gagttggccg gcgatcggga
      961 ttcagatcga gggacggggt ccgattctac gaacgacggc gtcgctgacc tactcgacgc
     1021 cactggactt gaagctcgat ccgtggcgca tccccacgca tactctgggc gatccggtcg
     1081 tcggtttcac agccctgcgg gggattcgcc catggctgag ccggcaggcc tggtggaaac
     1141 aactggagtt gcccaccaca cccaatcagg ctgtcggttg gacgatcgcc gagatcccgt
     1201 ttgcctccta tgtcgcttgg gaagcgcccg aggtgacaaa tgtcatgacg cagctcggtc
     1261 cgaaagtgtc cgccgttttg gaacggtaca tcccgaaggt ccggtttggc gacgccatgt
     1321 tccagaccaa cacaacccgt ctggcctgga ctaacctgcc gatcgccgag ccgtttgtca
     1381 gccccgccac cgcccaggat cccgggttcg ccgttgcggg tcttttcccc ctggtcaaga
     1441 ccaagcgccc actcccgccc gaactgctgt cccagatcat ggatcggatc gacctggtct
     1501 attatagctg ggagttgacc gcccaacgcg tcaacggctg gcgacacatc aagaacctgt
     1561 actggatgct ggcaggctac gcccccctgc cgaccaatta caccggtgag gcgtggttgt
     1621 ttgacaccaa cgttgtcgat cgtttgggca ataccggcac cgaactcagg ctcgagtccc
     1681 cgcgtcactt ggtcggggtg cgaaattccg cgctgggcct cagcgggctt gaacttgtgg
     1741 cgctcatgcg gtggctggat gacccggact ttccgcgcct cagcgagccg gcggcgcgcc
     1801 cggcgctatt gcaccgcatg cgcgccaggc agaccggcaa accgggcgcg ggcgttcccc
     1861 ccgcatcccc tgccccatcg aatgcccccg cggctccgcc cgtccgccca tccccctgat
     1921 gtcgccatgc tcaaacttgg ggtgaacatc gaccacgttg ccacgctgcg cgaggcccgc
     1981 taccgcggcc tggatgccgg cgaaccggac ccggtggatg ccgcccgacg ctgcgaagcg
     2041 gccggagcgc acggcatcac agcacacctg cggaaagatc ggcgacacat ccaggaccgc
     2101 gatgtgtggc ggctgcgaga gacgattcgg acgcgattga atctcgagat ggccaattcg
     2161 ccgccgatcg tggatttggt tctcgaactc aagccggaga tcgtctgcct ggtgccggaa
     2221 cgccggcagg aggtcaccac ggaaggcggc cttgatgtcg cgggccaatg cagcgcgctg
     2281 gcgagaaccc gccgccgcct caacaaggcg ggaatcgagg tgagtctttt tatcacgccc
     2341 gatctgcacc aggttgccgc cgccgcccgc gtaggcagtc agtttgtcga attgcacacg
     2401 ggccgctttg ccaaacactt cgagaatcta gccggcagaa agcgcgagct cag
//
LOCUS       DAOQRO010000418         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_51966_length_2453_cov_3.399083, whole genome shotgun sequence.
ACCESSION   DAOQRO010000418 DAOQRO010000000
VERSION     DAOQRO010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51966_length_2453_cov_3.399083"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..673)
                     /locus_tag="PKK20_12535"
     CDS             complement(239..673)
                     /locus_tag="PKK20_12535"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNV00758.1"
                     /translation="MTDQSASSASTPDASRHQTALFANLVIQQTNMALIFLGQAPHPQ
                     TGTTETDVEAASVFIDTLDMLAAKTKGNLSKSENDLLQQSLTNLRMAFVEAADASPPK
                     PSPPSSAPGDAPKDSPPASAPSAAPSDTPPEDDPRKKFVKKY"
     gene            complement(666..1370)
                     /locus_tag="PKK20_12540"
     CDS             complement(666..1370)
                     /locus_tag="PKK20_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNV00759.1"
                     /translation="MELPTEVPVMTLPNVILFPQAMLPLYIFEPRYRRMLSDTLETHR
                     MIAVAMQKPGRVRECPANVAGLGLVRASVKKRDGTSNLVLQGIARVELMRRVPHRPYR
                     IHGIRYLPRLAASAQAVQALAVRLLDLVTERLKLGFEPPFKTLAELTADAGLGAGPAT
                     PDETATEAFREVLRHLSQVDDPEQLVDLISATLLPSPRERQVILETVHLEDRLRHLVS
                     FLYHDIERRKSSENHD"
     gene            1530..>2453
                     /locus_tag="PKK20_12545"
     CDS             1530..>2453
                     /locus_tag="PKK20_12545"
                     /inference="COORDINATES: protein motif:HMM:NF020086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNV00760.1"
                     /translation="MNVPQHSGAVDSKGFLPMTRAEMSAWGWDALDILIITGDAYVDH
                     PSFGAAVIGRVLEAEGFRVGIVAQPDWRRLEAIEVMGRPRLFCGVTAGNLDSMLANYT
                     AARHRRKEDVYSEDGLPGRRPNHAAIVYAQMCRRAFPGLPVVLGGLEAGMRRFAHYDY
                     WEDKLRPSLLADAKADILVYGMGEVAVREIARRLRAGVTDLGGIRGTARLLGGRETST
                     VGFGDCIRLASWEELRRDPKLLLRLTKVVEAQQTPYCGKRLVQAHGNRALLQEPPALP
                     LDGRDLDAVYELPFMRAPHPGYRGRIPAFAAI"
BASE COUNT          419 a          692 c          862 g          480 t
ORIGIN      
        1 aagaaagtcg ccccggtgcg cttcaagcag gccgagttcc cgatccctcg ctatcacccc
       61 gaacacctcc gcgggcgcgg cgagttggtg gtctacgaaa aggcggaacg cgtctaactc
      121 ggatttctca acacttgaag gagtaatctg aaatacgggc tgacatttgc acttctcccg
      181 tgtactccgg cttcgcgttg gtcaaaagtg gtgagaaatg caggctaacc cggaccggtc
      241 agtatttctt gacgaacttt ttgcgcggat cgtcctcggg cggcgtgtcc gagggggccg
      301 cactcggtgc ggaggcgggg ggagagtctt tcggggcgtc gccgggcgcg gacgagggcg
      361 gactcggttt cggcggagag gcatcggcgg cttcgacgaa ggccatgcgc aagttggtca
      421 ggctttgttg gaggaggtcg ttctcgctct tcgagaggtt gcctttggtc tttgcggcca
      481 gcatatcgag cgtgtcgatg aagacgctgg cggcctcgac atcggtctcg gtggttccgg
      541 tttggggatg aggcgcttgg cccaggaaga tgagggccat gtttgtctgt tggatgacaa
      601 gattggcaaa cagggccgtt tgatgccggg aggcatccgg cgtcgaggcg gatgaggcgg
      661 attggtcagt catggttttc ggaggatttg cggcgctcga tgtcgtgata gaggaagctc
      721 accaaatggc gcaaccgatc ctcgaggtga acggtttcga ggatgacctg gcgctcgcgg
      781 ggcgagggca acagggtcgc ggaaatcagg tcaaccagtt gctcgggatc gtcgacctgg
      841 ctgaggtgcc gcagcacttc acgaaaggcc tccgtggccg tttcgtccgg agtggccggt
      901 cccgcgccaa gtccggcgtc ggcggtcaat tcagccagag tcttgaacgg cggttcgaag
      961 cccagtttga ggcgctcggt caccaggtcg agcaagcgga cagccagcgc ctgaaccgcc
     1021 tgcgctgacg ccgccaggcg cggcaggtat cggatgccat gaatgcggta tggccggtgt
     1081 ggaactcggc gcatcagttc gacccgggcg atgccctgga gcaccaggtt cgatgtcccg
     1141 tcccgtttct tcaccgaggc tcggaccagc ccgagcccag ccacgttcgc cgggcattcc
     1201 cggacgcgtc ctggtttctg catggccacg gcgatcatgc gatgggtttc gagtgtgtcg
     1261 gacagcatcc ggcggtaccg cggctcgaag atatagagcg gcagcatcgc ctgcgggaac
     1321 aggatcacgt tcggcaaggt catcaccggc acctcggtcg gcagttccat atgtggattc
     1381 tagtctgcag tgcgtgtaat gcaagaaggc gcgagcctgc cttgccggcg gcgggcggtc
     1441 gccgctccac gttatgcatt ggcgctgctc gtgatgccaa tcgctgattt gcgtctggca
     1501 aatccatgct tcattcttgg agaagcgaca tgaacgtccc gcaacactca ggcgccgtgg
     1561 acagcaaggg cttcctcccg atgacccggg cggagatgtc cgcatgggga tgggatgcgt
     1621 tggacattct gatcattacc ggggacgcct atgtcgatca cccgtctttt ggggcggctg
     1681 tgatcggacg ggttctcgaa gccgagggat tccgcgtggg catcgtagcg cagccagact
     1741 ggaggcggct tgaagcgatc gaggtcatgg gacggccccg cctcttttgc ggcgtgacgg
     1801 cagggaatct ggactcgatg ctcgccaatt acacggccgc gcggcacagg cgcaaggagg
     1861 acgtttacag cgaggatggc ctgccgggga ggcgcccgaa ccatgcggca atcgtctatg
     1921 cgcagatgtg ccggcgggct tttccaggcc tgccggttgt tctgggggga ctcgaagccg
     1981 ggatgcggcg gttcgcgcat tacgactatt gggaggacaa gctgcgtccg tccctgctgg
     2041 ccgacgcaaa ggcggacatc ctggtttacg gcatggggga agtcgccgtg cgcgaaatcg
     2101 cgcgccggct gcgcgcgggt gtgacggatc tgggcggcat tcgcgggacg gcgcgcctgc
     2161 tcggcggacg ggagacctcg acggtcggtt ttggagactg catccggctg gcctcgtggg
     2221 aggaattgcg gcgcgatccc aaacttctcc ttcgcctgac caaggtggtt gaagcgcagc
     2281 agacgcccta ctgcggcaag cgattggtcc aggcgcatgg gaaccgcgcc ttactccagg
     2341 aaccgcccgc cttgccactg gacgggcgcg atctggacgc ggtctatgag ttgccgttca
     2401 tgcgggcgcc gcatccgggg tatcggggca gaatcccggc cttcgccgcg att
//
LOCUS       DAOQRO010000419         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_52212_length_2445_cov_3.434728, whole genome shotgun sequence.
ACCESSION   DAOQRO010000419 DAOQRO010000000
VERSION     DAOQRO010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52212_length_2445_cov_3.434728"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKK20_12550"
     CDS             complement(<1..198)
                     /locus_tag="PKK20_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNV00761.1"
                     /translation="MKRTFLFNLAVALLGGAFSATAAEATLGIGDPAPKLAVGEWVQG
                     EPVKEFQKDKTYLVEFWATWCA"
     gene            complement(337..>1151)
                     /locus_tag="PKK20_12555"
     CDS             complement(337..>1151)
                     /locus_tag="PKK20_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00762.1"
                     /translation="GNDTTAAESWAYLYVPCEATPQAWEHILKRAPRCVEWSEAEVMA
                     LVGEEKFSQTMDVIRRISTPELMRFKVGRDGLGLRPPQSGGSAVAILGGTDAGPALAV
                     GGFCRQQLEATRRAGGRIGLICLAQPPALETVRAAVRDDWAADCLVVLVPAPEGRLLL
                     DGAARVGLKLLLNALSTCTMVRLGRVMGNFMIWVVASNLKLIDRATRYIQRLTGLEYT
                     DANRLLFEVVEYVEPRCKADKAYPPVVGLAVMRQRHGLTNEQAEERLMAELG"
     assembly_gap    1152..1161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1162..2200)
                     /locus_tag="PKK20_12560"
     CDS             complement(<1162..2200)
                     /locus_tag="PKK20_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00763.1"
                     /translation="MSNHATNRSAEFLKIADQFKLGVLVTESSHPVTANLSDASRRDI
                     KAGLRQLFDVDGDVVRKYREFVQSGRAASIAQTVLRALEQGGKLFFTGCGSTGRLSIQ
                     LVSIWRDFWQRQRARGLACHPEPSDWENRASSVMAGGDFALIKAVEGFEDFTAFGKKQ
                     IGDLGVAKGDVVFAITEGGETSFVIGTAWRGVEAGARVFFVYNNPDDILCQHVQRSRE
                     VILDPRIEKINLTTGPMAITGSTRMQATSIQLAVMLTVLEMVLRELMCRLHPDGPDAL
                     DPSTVPSEFLDQLTRMHATLKTPEVLDPLAGLVALEEAVYRAGRRNNYFADRFGIDVL
                     TDTTERSPTFCT"
     gene            2199..>2445
                     /locus_tag="PKK20_12565"
     CDS             2199..>2445
                     /locus_tag="PKK20_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00764.1"
                     /translation="MAGKMANAIPQGNAAFESLDTRCRSGWGFRARMLQPRTNHILAR
                     APGENRLQPRHLRVCAKITWKALTFNGLMRIEPMNRGT"
BASE COUNT          453 a          805 c          738 g          439 t
ORIGIN      
        1 cgcgcaccat gtcgcccaga actcgacaag gtaggtcttg tccttctgga actccttaac
       61 gggctcgcct tgcacccact ctcccacggc cagcttgggt gcgggatcgc cgatcccaag
      121 cgttgcttca gcggcggttg cggagaatgc gccgccgaga agagcgacgg ccaggttgaa
      181 taggaatgtg cgtttcatga acttgagggg gttgcgttcg tcaaataagg cttggtatgc
      241 cgatcaagga tgcgagcgta gcctcgatcg accggaataa caagcgggat tggacgaata
      301 cactggaccg cgccgctctt cgaaactgtc cgtgattcag cccaactcgg ccatcagccg
      361 ctcctcggcc tgttcgttag tcaatccgtg gcgctgacgc atcactgcca gacccaccac
      421 gggcggatag gccttgtcgg ctttgcagcg cggctcgaca tattcgacca cctcgaacag
      481 gagccggttg gcgtccgtgt actcgaggcc ggtcaaccgc tggatgtaac gcgtcgcccg
      541 gtcgatcagc ttgaggttgc tggccacgac ccagatcatg aagttgccca tgacccggcc
      601 caaccggacc atggtgcagg tggaaagggc gttgagcagg agcttcaatc ccacgcgtgc
      661 cgctccatca agcaggagcc ggccctcggg tgccggcacc aggaccacaa ggcagtccgc
      721 cgcccagtcg tcgcgcaccg ccgctctcac cgtttccaac gccggaggct gggccaggca
      781 aatcaagccg atcctgcctc cggcacgtcg cgtcgcctcg agttgctgtc tgcaaaaccc
      841 gccgacggcc agcgccggcc ccgcatcggt cccgcccaga atcgccaccg cgctgccgcc
      901 ggactgaggc gggcgcagcc ccaaaccgtc ccgaccgacc ttgaaccgca tcagttccgg
      961 tgtgctgatt ctgcggatca cgtccatcgt ctgggagaac ttctcctccc cgaccagtgc
     1021 catcacttcg gcctcgctcc actcgacgca ccggggcgcg cgtttgagga tatgctccca
     1081 ggcctgcggg gtcgcctcgc aaggaacgta caggtacgcc catgattcgg ccgccgtcgt
     1141 gtcgttgccc tnnnnnnnnn ngcgtgcaga aagtgggcga ccgttccgtg gtgtcggtga
     1201 gcacgtcgat gccaaaccgg tcggcgaagt agttgttgcg ccggccggcg cggtagacgg
     1261 cttcctcgag tgcgacgagc ccggccaagg gatcgagaac ctcgggcgtt ttcagggtcg
     1321 catgcatccg ggtcagttgg tccaggaact ccgacgggac cgtggacggg tcgagggcgt
     1381 cgggtccgtc cggatgcagg cggcacatca actcgcgcag caccatctcg agcacggtca
     1441 gcatcaccgc caattggatg ctggtcgcct gcatccgggt ggaccccgtt atcgccatcg
     1501 ggccggtggt cagattgatc ttctcgatcc gcggatccag gatgacttcg cggctgcgct
     1561 gcacgtgttg gcagaggatg tcatcggggt tgttatagac aaagaaaacg cgcgcccccg
     1621 cctcgacccc gcgccaggcc gtgccgatga caaacgaggt ttcccctccc tcggtgatgg
     1681 cgaaaaccac gtcgcccttc gccactccga gatcgccgat ctgtttcttg ccgaaagccg
     1741 tgaaatcctc gaagccttcc acggccttga tgagggcgaa gtcgccgcct gccatcacac
     1801 tcgaagcccg gttctcccaa tccgagggtt cggggtggca ggccaggccc cgggcgcgct
     1861 gtcgctgcca gaaatcgcgc cagatcgaga cgagttgaat gctcagacgc cctgtcgagc
     1921 cacagccggt gaagaacagc ttgccgccct gttccaaggc ccgcaacacg gtctgcgcta
     1981 tactggccgc tctgcccgac tggacgaact cgcggtactt ccggaccacg tcaccgtcga
     2041 catcgaacaa ctgcctcagc ccggccttga tgtcgcggcg gctcgcgtcg ctcagattgg
     2101 ccgtcaccgg atgggacgat tcggtcacca gaacgcccag cttgaattga tccgcgatct
     2161 tcagaaactc cgccgaccgg ttcgtggcat ggttgctcat ggcgggcaag atggccaatg
     2221 ccatccctca aggcaatgcc gcattcgagt cgctcgacac acgatgccgc tccggatggg
     2281 gattccgggc gcggatgctg cagccgcgca ccaatcacat tttggcccgg gcccccggcg
     2341 agaaccggct gcagccgcga cacctaaggg tctgtgcaaa aattacatgg aaagcattga
     2401 cattcaacgg tttaatgcgc attgaaccca tgaaccgggg aacgt
//
LOCUS       DAOQRO010000420         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_53549_length_2407_cov_4.795493, whole genome shotgun sequence.
ACCESSION   DAOQRO010000420 DAOQRO010000000
VERSION     DAOQRO010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53549_length_2407_cov_4.795493"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..995)
                     /locus_tag="PKK20_12570"
     CDS             complement(81..995)
                     /locus_tag="PKK20_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00765.1"
                     /translation="MTSPQLVTLKDGEGGSATIAPGLGGWLLRYSREFVAHGRVEALH
                     FDPAVIERYPREMYAGSPVLFPLASFNHVPGRDHHYEWQGRTYEMLQHGFARRHPWTV
                     LDQTDASVTIELTDSEATLAQFPFEFSLQLTYRLAGGRLFWQQKVINRSPDPLPFTTG
                     FHPYFAVPLAPRGLRSECFVEIPDCRRLTMHGRGDQFTVKPFPSQNWSVAEDVSMTLF
                     LSDLKRRELVLVDPISELEVALDWRDAPAFRFVAIWSKSTEAPFYCLEPWTAVSNPFT
                     RVKDRELIVLEPGKDFEAAFRMELRETP"
     gene            1131..1757
                     /locus_tag="PKK20_12575"
     CDS             1131..1757
                     /locus_tag="PKK20_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV00766.1"
                     /translation="MPIAVSIVEDSEQVRQTLARLINRAEGFRCASQYGSAEDAIAGL
                     PRDRPDVVLMDINLPGMNGVECVRRLKKTIPEVQFLMLTVYEDTDNIYNALLAGATGY
                     LLKRTSRDELLAAIRDVHRGGSPMTTHIARKVVQSLQRAGASPQPAENLSPREQEVLD
                     DLARGFLYKEIADNLGISYETVHTYIRRIYEKLQVRTRTEAVAKFLGR"
     gene            complement(2028..>2407)
                     /locus_tag="PKK20_12580"
     CDS             complement(2028..>2407)
                     /locus_tag="PKK20_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00767.1"
                     /translation="ANEPVSDEEIETARKSFIDTFPRTFGTKGRIAATFAADEFTGRY
                     ARQPDFWKTYRDKVAAVTKEDVRRVAQKYLTPDKLVVLVVGQKQEILKGHPDHAVQLG
                     DLVGNRFTDVPLRDPLTLKPLAQ"
BASE COUNT          473 a          777 c          738 g          419 t
ORIGIN      
        1 tgcgggtatg ctccctcgtc gcgcctcgcc agcgagccaa atcccctcca gcaaaaccgg
       61 aatcggtttt tgcacagaca ctaaggcgtt tcgcgcaatt ccatgcggaa cgcggcctcg
      121 aagtccttgc ccggttcgag cacaatgagt tcacggtcct tgacccgtgt gaacggattc
      181 gacacggccg tccagggctc gagacaatag aatggcgcct cggtcgactt ggaccagatg
      241 gccacaaacc ggaacgccgg cgcatcgcgc caatcgaggg ccacttcgag ttcgctgatc
      301 ggatcgacca ggacgagttc gcgccgtttc agatcggaca gaaacagggt catcgacacg
      361 tcttccgcca cgctccagtt ctggctcggg aacggcttga cggtgaattg gtctccgcgt
      421 ccgtgcattg tcagccgccg gcaatcggga atctcgacga agcactcgga acgcagcccg
      481 cgaggcgcga gcgggacggc gaagtaggga tgaaaacccg tggtgaaagg gagcgggtcg
      541 ggcgagcgat tgatcacctt ctgctgccag aacaaccgcc cgcccgccag ccggtaggtc
      601 aactgcagcg agaactcgaa cgggaactgg gccagcgtcg cctcgctgtc cgtcaactcg
      661 atcgtcaccg acgcgtcggt ctggtccagc accgtccacg gatggcggcg agcgaagcca
      721 tgctgaagca tctcgtatgt gcggccctgc cattcgtagt gatgatcgcg cccggggacg
      781 tggttgaaag aggccagggg aaacaacact ggagaccccg cgtacatttc ccgcggataa
      841 cgttcgatca cggccggatc gaagtggagg gcctcgacgc ggccgtgcgc gacaaactcg
      901 cggctgtacc gcagcagcca accacccagc cccggagcga ttgtggctga acctccctcg
      961 ccgtctttga gtgtcaccaa ttgaggactc gtcataacga acagcagtct attgttgttc
     1021 gacaaaataa tccactccgg tctggctccg gacgaattga cgcgcggtcc ggttgcaggt
     1081 aacactgcgt ggcggcagcg ggggacaaag ccccgcccgc cgcattcacc atgccgatcg
     1141 ccgtatccat tgtcgaggac agcgagcagg tgcgtcagac cctggcgcgc ctgattaacc
     1201 gcgccgaggg cttccgctgc gccagccagt acggcagcgc cgaggacgcc attgcgggcc
     1261 tgccgcggga tcgtccggat gttgtgctga tggacatcaa cctgccgggc atgaacggcg
     1321 tcgagtgtgt tcggcgtctc aagaagacaa tccccgaggt gcagttcctg atgctgacgg
     1381 tctacgagga cacggacaac atctacaacg cgctgttggc gggcgccacg ggttatctgc
     1441 tcaagcgcac atcgcgcgac gagttgctgg ctgccatccg cgatgtgcac cgcggcggct
     1501 cgcccatgac gacgcacatc gcccgcaagg tggtccaatc gctccagcgc gcgggcgcct
     1561 cgccccaacc ggcggagaac ctctcgccgc gcgagcagga ggtgctcgac gacctggctc
     1621 gcggcttcct ctacaaggag atcgccgaca acctcggcat cagctatgaa accgtccaca
     1681 cctacatccg gcgcatctac gagaaactcc aggtgcgcac ccggacggaa gcggtggcca
     1741 agttccttgg acgttgacta acccgcatct ttcacgcccc gccggagccc agggcgctca
     1801 gacctcgcag gatgactgct atgagggttg gaaaagcaaa accgcgccgg gtctgagaca
     1861 tccaggaatg cagtacactg gtccgacgac agaacgcccc cagaaccgac tagacgcccc
     1921 cggttctctt tctgggcgcg gttttgcctg agccgggata ggcggccccg cgaagccctg
     1981 aaatacccgc gccgaagccg tccggcgacg cgcttcgatc ggcagcttca ctgcgccagg
     2041 ggcttgagtg tcagcggatc gcgcaaaggc acgtcggtga atctgttccc caccaaatcc
     2101 cccaactgca cggcgtggtc gggatgaccc ttgagaattt cctgtttctg tccgaccacg
     2161 agcaccacca gcttgtccgg cgtcaggtac ttctgggcga cgcgccggac gtcttccttg
     2221 gtcaccgccg caaccttgtc gcggtaggtc ttccagaaat cgggctgacg ggcgtaacgg
     2281 ccagtgaatt cgtcggcggc gaaagtggcg gcgatcctcc ccttcgtgcc gaaagtccgg
     2341 gggaaggtgt cgatgaacga cttcctggct gtctcgattt cctcgtcgct gaccggttca
     2401 ttcgcga
//
LOCUS       DAOQRO010000421         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_54514_length_2380_cov_3.892043, whole genome shotgun sequence.
ACCESSION   DAOQRO010000421 DAOQRO010000000
VERSION     DAOQRO010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54514_length_2380_cov_3.892043"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKK20_12585"
     CDS             complement(<1..722)
                     /locus_tag="PKK20_12585"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV00768.1"
                     /translation="MRPATTDSTKAYEFLAWLELNKKRLAFGIGIGLLLFVVVYVYSH
                     FRGQKELAGNRALLALQAKQAGAKDSVVPAADLLKLAGEYESTSAGERALLLAAGNLF
                     AQGDYAQARKYFEQFQKDNSGSPLAPLAAFGAAASLDALDQVDAALAAYQAVVNQFPE
                     DAVALRARLAMGTLHEAKNQPEQAHRIYDQLSKQIGVGRIASEAGELLEKLERRFPHL
                     NQPTNAPVASATSRRRTPSTPRP"
     gene            820..>2380
                     /locus_tag="PKK20_12590"
     CDS             820..>2380
                     /locus_tag="PKK20_12590"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNV00769.1"
                     /translation="MAEAAVTSTLRLRGVRHNNLKNFDLDLPLGRLIAITGLSGSGKS
                     SLAFDTLFAEGQRRYIETFSPYARQFFDRMDKPQVDCIEGIPPAIAIEQRNAVRSTRS
                     TVGTMTEICDYMKMLWPHVAQLHCRQCGRPVRQDTPQQIWRALRAETNGCDRDVLITF
                     GLALSDRLDLAESLELVAKQGYQRLLLDGRIRRLDEVASERLAVERSLTRLTVIQDRL
                     RLADRNRARFIEACEQGLHFGKGKLAVYGFGGAGDLHAASASTGAVTQERPDAVVGPA
                     RLFSNRRHCAECDLEYREPSPALFSYNHPLGACPTCKGFGRTISIDSRRAIPDRTRTL
                     AGGAVRPWQSGVSAECQKDLLKFCRMRGVPTDVPFNRLSPEHQRWVIEGDPGYGTDSE
                     HEWPRAWYGVLGFFRWLESKAYKMHVRVFLSRYRSYRPCPDCRGRRLTPEALLYRIAV
                     PTRAPIARATSDAQGRKAGIASTESGLTLADFYALPIQDALAVANELIARVGAKPSDA
                     LGLALGEVRARL"
BASE COUNT          406 a          805 c          731 g          438 t
ORIGIN      
        1 ggcctcgggg tcgatggcgt ccttctccgg gacgtcgcgg acgcgacggg tgcgttggtg
       61 ggctggttca ggtgagggaa gcggcgttcg agcttttcga ggagttcccc ggcctcgctg
      121 gcgattctgc ccaccccgat ttgtttgctg agctgatcgt agattcgatg tgcctgttcg
      181 ggctggttct tggcctcgtg gagagtcccc atggcgagcc gggcccggag cgccaccgca
      241 tcctccggaa actgattcac aacggcttga taagcagcca gcgcggcatc gacctggtcc
      301 agtgcgtcga ggctggcagc cgccccaaaa gccgcaagtg gagccaaggg gctgccggaa
      361 ttgtctttct ggaactgctc gaagtacttt cgggcctggg cgtagtcccc ttgggcgaac
      421 aggttgcccg cggccagcag cagagcgcgt tcgcccgcgc tggtggactc atactcgccg
      481 gcgagtttca gaaggtcggc cgccggaacg accgaatcct tggcgccggc ttgcttggcc
      541 tgcagcgcca acaaggcccg gttgcctgcc agttccttct gtccgcggaa gtgggaatag
      601 acgtacacca ccacgaataa cagcaggcca atgccgatcc caaacgccag ccgcttcttg
      661 ttcaactcga gccaagccag aaactcatag gccttcgtcg agtctgtcgt cgcaggtcgc
      721 ataaaagacc gtgattctct ggacgcacct cgaaaaggca agcgggaaat ccctcgcgtt
      781 cgttgacaca tccggcgtcc cgtttacact gcgcccgcta tggcagaggc ggcagtcacc
      841 tccacgctcc ggttgcgcgg ggtccggcac aacaacctga agaacttcga cctcgacttg
      901 cccttgggcc gcctgatcgc gatcaccggc ctgagcggtt caggcaagag ttctctggct
      961 tttgacacac tcttcgccga agggcaacgg cggtacatcg agaccttctc accttacgcg
     1021 cgccagttct tcgaccgcat ggacaaaccg caggtcgact gcatcgaggg tatcccgcca
     1081 gccattgcga tcgagcagcg aaatgccgtc cgcagcaccc ggtcgaccgt gggcaccatg
     1141 accgaaatct gcgattacat gaagatgctc tggccgcacg tcgcccagct ccattgccgt
     1201 cagtgcgggc gcccggtccg acaggacacc ccccagcaga tctggcgggc gttgcgcgcc
     1261 gaaacaaacg gttgcgacag ggacgtgctg atcacctttg gcctcgctct ctcggaccgg
     1321 ctcgatctcg ccgaatcgct cgaactggtc gccaaacaag gttaccaacg gctgcttctc
     1381 gacggtcgca ttcggcgcct cgacgaggtc gcatccgaac gcctcgccgt cgagcggtcg
     1441 ttgacccgat tgactgtgat tcaggatcgc ctgcgtctgg ctgatcgcaa ccgcgcgcgg
     1501 ttcatcgagg cgtgcgagca ggggctccat ttcggcaaag gcaagctcgc cgtctacggc
     1561 ttcggcgggg caggggacct acacgccgcg tccgcttcga ccggggccgt cacccaagag
     1621 cgtccggatg ccgtggtcgg gccggcccgg ttgttctcaa accggcgcca ttgcgcggag
     1681 tgcgatctcg agtatcggga gccatccccg gctctattca gctacaacca tccgctcggc
     1741 gcctgcccca cgtgcaaggg gttcggacgg accatcagca tcgacagccg acgcgccatt
     1801 ccggatcgga cccgaacgct cgccggaggc gcggtgaggc cgtggcaaag cggcgtgagc
     1861 gctgaatgcc agaaggactt gctcaagttc tgccggatgc gcggtgtgcc gaccgatgtc
     1921 cctttcaacc ggctgtcccc cgaacaccaa cgctgggtca tcgaagggga tcccggctac
     1981 ggaaccgact ccgagcacga gtggccccga gcctggtacg gcgtcctggg cttttttcgc
     2041 tggctcgagt cgaaagccta caagatgcat gtccgcgtct tcctgtcccg ctaccgcagc
     2101 tatcgccctt gtccagactg ccgtggccgc cgtcttacgc ccgaggctct gctctaccgc
     2161 atcgctgtgc cgacccgcgc ccctatcgcc cgagcgacct ccgacgcgca gggccgcaag
     2221 gccggcattg cctcgaccga atccggtctc acgctcgccg acttctatgc cctgccgatt
     2281 caggacgcgc tggccgtcgc caacgaattg atcgcgcgtg tcggcgccaa gccctccgac
     2341 gccttggggc tcgcgttggg cgaagtccgc gcccgcctcg
//
LOCUS       DAOQRO010000422         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_54971_length_2367_cov_3.722318, whole genome shotgun sequence.
ACCESSION   DAOQRO010000422 DAOQRO010000000
VERSION     DAOQRO010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54971_length_2367_cov_3.722318"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1707)
                     /gene="ccsA"
                     /locus_tag="PKK20_12595"
     CDS             complement(28..1707)
                     /gene="ccsA"
                     /locus_tag="PKK20_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV00770.1"
                     /translation="MNKWLPWIVTGVFGLWALSGLRPPRPATDFDSIGFGRLPTVLNG
                     RLQPLDSVARNSLLLLCGKQSVGLGRGARMPAIDWLLEVAMKPEVADGRAAFKIDHPE
                     LRGLLDLAPDGSLYVGFDAIRSRVEEIETQARRASAVPVENRSSFEKAVVRLSSGVSL
                     YHRLKNSFRPEDTEDFAAVVAAYERSLGPGLAAWRLRQENRPFSQEDLDRLSKALQGF
                     DVLSSLAYPLLIPPRDLQTGREPWSNLGTSLIEAIHAGEIHPAVRFYAAMASAYRLGK
                     AEEFNRALADYRGWLAGPFALEVRKASREFIYNAWRPFVVSLALYLLAFLAGCASWFG
                     WFQVLNRTAIKLLAVGFLVHTAGIVFRMALEGRPPVTNLYSSAVFVGWGAVLLGLIIE
                     RLNRDSIGVVMAAALGLLTQIVAHNLSLGGDTMEMLRAVLDSNFWLTTHVITITLGYS
                     AMFVAGFLGIVYVVRRAFTGTFTEAIGRRLARMVYGVICFATLFSFVGTVVGGIWADQ
                     SWGRFWGWDPKENGALLVVLWCAIILHARGGGLVRERGLINLAIFGNVVTN"
     gene            complement(1704..>2367)
                     /locus_tag="PKK20_12600"
     CDS             complement(1704..>2367)
                     /locus_tag="PKK20_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNV00771.1"
                     /translation="RLARHKQIRHPGLPFEVRVIEYWPNSTHRPIEPGQGTARATQGA
                     GQRVQFVSATPETRMDRRNIPAAYVELVASGGSLGVWAVSLWIGAGQEFQHEGKNYEI
                     ALRPARHYRPFALKLLRFSHDKYMGTDIPSNFSSDVHLSNARTGESRDVRIYMNNPLR
                     YGGETFYQSGYDQQDPRVTILQVVRNPGWLTPYFSCVLVGAGLVVQFLSHLVSFVKRR
                     NA"
BASE COUNT          503 a          787 c          701 g          376 t
ORIGIN      
        1 tcgtatagga acgcggtcac actcagatca gttcgtcacc acgttcccga agatcgccag
       61 gttaatcagc ccgcgctccc gaaccagacc gccccctcgg gcatggagga taatggcaca
      121 ccagagcacc acgagcagcg cgccgttctc cttgggatcc cacccccaaa accggcccca
      181 ggattgatcg gcccaaatgc cgcccaccac cgtgccgacg aagctgaaaa gcgtggcgaa
      241 gcagatcacg ccatagacca ttcgggccaa ccgcctgccg atcgcctcgg tgaatgtgcc
      301 ggtgaacgcc cgccgaacca cgtagacaat gccgagaaac cccgccacga acatcgccga
      361 gtacccgagg gtgatggtta tgacatgcgt cgtcagccag aaattgctgt cgaggaccgc
      421 gcgcagcatc tccatcgtgt cgccgcccaa cgacagattg tgcgccacaa tctgggtcag
      481 aagacccaac gccgccgcca tgaccacgcc gatgctgtcg cggttgagcc gctcgatgat
      541 caaaccgagg agcactgcgc cccacccgac aaatacggcg gacgagtaca gattcgtgac
      601 cggcggtcgc ccttcgagcg ccatgcgaaa gacaatcccc gccgtgtgca cgaggaatcc
      661 gacagccaac agcttgatcg ccgtgcggtt gagcacctgg aaccagccaa accacgacgc
      721 gcacccggcg agaaacgcca ggagatagag cgccagactg acgacaaacg gacgccatgc
      781 gttgtagatg aactctcgag aagcctttcg cacttccaac gcgaaaggcc ccgcaagcca
      841 gcctcgatag tcggccaaag cccgattgaa ttcctccgct ttgccaagtc gatacgccga
      901 ggccatggcc gcatagaagc gaaccgcggg gtgaatctcg cccgcgtgga tcgcctcgat
      961 caggctggtt cccaggttcg accacggctc gcgcccggtc tggaggtcgc gcggcgggat
     1021 cagcaagggg taagccaggc tgctcagaac gtcgaagccc tgcagcgcct tggagagcct
     1081 gtcaagatcc tcctggctga atggcctgtt ctcctggcgg agccgccacg ccgccaaacc
     1141 cggaccaaga gacctctcat aagcggcgac gacggcggcg aagtcctcag tgtcttcagg
     1201 ccggaagctg ttcttgagcc ggtgatagag cgagaccccg ctggagagcc tcacgaccgc
     1261 tttctcgaaa ctgctgcggt tttcaaccgg caccgcgctc gcgcgacgcg cctgcgtctc
     1321 gatctcctcg acccggctcc gaatcgcgtc gaaaccaaca tagagcgagc cgtccggggc
     1381 taaatccaga agaccgcgca actccgggtg atcgatcttg aaggccgccc ggccatcggc
     1441 gacctcgggc ttcatggcga cttcgaggag ccaatcgatg gccggcatcc gggcgccgcg
     1501 gcccaatccc accgactgct tgccgcaaag aagcagcagg ctgttccgcg ccacggaatc
     1561 gagcggctgc aaacgcccgt tcagcacagt cggtagacgc ccaaatccga tgctgtcgaa
     1621 atcggtggcg gggcggggag gacgcagccc gctcagggcc cagaggccaa agacaccggt
     1681 gacaatccac ggcagccatt tgttcatgcg tttcttcgtt tcacgaagct cacgaggtga
     1741 ctgagaaact ggacaaccag ccccgcgccc accagcacgc acgagaaata aggcgtcagc
     1801 cagcccggat ttcggaccac ctgcaggatc gtcacgcgcg ggtcctgctg gtcgtagcca
     1861 gattggtaga aagtctcgcc tccgtaccgc aacgggttgt tcatgtagat gcgcacatca
     1921 cggctttcgc ccgtgcgcgc attgctcaga tgtacatcgc tcgagaagtt actcgggatg
     1981 tccgtgccca tatacttgtc atgcgagaat cgcagcaatt tgagggcgaa gggccggtaa
     2041 tgccgcgcgg ggcgcagcgc gatttcgtag ttcttgccct cgtgttgaaa ctcctggcct
     2101 gcaccgatcc agagcgacac ggcccagacg cccagggatc cgccggacgc aaccaactcg
     2161 acataggcag cggggatgtt gcgccgatcc atccgcgtct cgggcgtcgc ggagacgaac
     2221 tgaacgcgct ggcctgcgcc ctgggtggct cgggccgtcc cctgccccgg ctcgatcggt
     2281 ctgtgcgtcg agttgggcca atactcgatc acacgaacct caaatgggag gccgggatgg
     2341 cgaatctgct tgtgtcgggc caagcgc
//
LOCUS       DAOQRO010000423         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_56715_length_2321_cov_4.525596, whole genome shotgun sequence.
ACCESSION   DAOQRO010000423 DAOQRO010000000
VERSION     DAOQRO010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56715_length_2321_cov_4.525596"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="fabF"
                     /locus_tag="PKK20_12605"
     CDS             <1..937
                     /gene="fabF"
                     /locus_tag="PKK20_12605"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNV00772.1"
                     /translation="FLGSGIGGLHTTEEQHKILLSKGPGRLSPFMIPMLILNMASGLF
                     SMFHGLRGPNLATCSACATSSHAIGEAWRTLKMGDADVMFAGGSEATITPLGIGGFCA
                     MRAMSTRNAEPKRAARPFDADRDGFVMGEGAGVLVLENLEHARRRGARIYCEVMGYGN
                     TADAHHMTVPSPEGEGAARCMRMALRSAGMAPEQISYINAHGTATPQGDICEAQAIRT
                     VFGAHASRLAVSSTKGATGHMLGAAGAVEMAVCSKAIQTGVVPPTLNLDHPDPACDLD
                     LVPHAAREMTVDAIVNNSFGFGGHNACLVARRYVG"
BASE COUNT          415 a          758 c          701 g          447 t
ORIGIN      
        1 cttcctcggc tcggggattg gcggcctgca taccactgaa gaacaacaca agattctgtt
       61 gagcaaggga ccgggccgcc tgtcgccctt catgatcccg atgctcattc tgaatatggc
      121 gtcgggcctc ttctcgatgt tccacggcct gcgcggcccc aatctggcga cctgctccgc
      181 atgcgcgacc tcctcgcatg ccatcggcga ggcatggcgc acgctcaaga tgggggacgc
      241 cgatgtgatg ttcgccgggg gcagcgaggc caccattacg ccgctgggca tcggcggctt
      301 ctgcgccatg cgggcgatga gcacgcgcaa tgcggagccc aaacgcgccg cgcgtccgtt
      361 cgacgcggac cgggacggct tcgtcatggg agaaggcgcg ggcgtgctgg tgctcgagaa
      421 tctcgaacat gcgcgccgac gcggcgcgcg aatctactgc gaggtgatgg gctacggcaa
      481 cacggccgac gcccatcaca tgacagtccc ctcgcccgag ggcgaagggg ctgcccggtg
      541 catgcggatg gcgctccgct cggcggggat ggcgccggag cagatcagtt acatcaacgc
      601 tcacggcacg gccacccccc agggcgacat ctgcgaggcc caggccatcc ggaccgtctt
      661 tggcgcgcat gccagccggt tggccgtcag ttccaccaag ggcgccaccg gtcacatgct
      721 cggagcggcc ggtgccgtcg aaatggccgt ctgctcgaag gcgatccaga cgggcgttgt
      781 gccccccacg ctcaacctcg atcatcccga cccggcctgc gacctcgatc tcgtgcctca
      841 tgcggcgcgg gaaatgacgg ttgacgccat tgtcaacaat tccttcggct ttggcggcca
      901 caacgcctgc cttgtggcac gcagatacgt gggatgagaa aagaaattgt ccctgcccga
      961 tcgcgggacc agacagggac aaatctggga gtgggagttc gccacgctct cttccccggg
     1021 ctggcattag gcgcctctcg attgggctgc cagcttcccg acgccattct ccacggcaaa
     1081 cggagttgtc ggcctcatag cagggccgtc cagcgccttg cggtcgctga attatcgagt
     1141 gaggcctctc ggctcgactg cgctggtgtt tgcgccaccg gcttgcgaac cgggcaaaga
     1201 cacgtctatg accgttgctt cgcgtctttg ccgcttgcac cgggtcggcc accgcgtgtg
     1261 gcggtgcgtt ggcccggtgc cacgggtctg acctcttacc tctcaagctg ccttacgctc
     1321 ataggccagc ggtgccgttt ttaacatcgc cagcgctttg ggaggttttc acaggacctg
     1381 cctcgccctg tgccctcccc gcgacagacg ctcgctttgt ctcgcctttc ggcgcatttc
     1441 gtcgggcggc cctcattcgg aacaaccgcc agagccgtcc gccgcgtgct ggcgcgcagt
     1501 agcagctacg caccctctgg gtgactgcga ttggctcgag cccgaaacca gcccgagttg
     1561 acttgactcg actcggagcg gcttctcatc gcccctcgtc gctcagttcc ttgctcgcgt
     1621 cactgacggc gcgcgaatgc cgccagagga actggtgaat cctgcctgca agacgcgcag
     1681 acctcacgat ccgctcttcc tcacaggggc tgggccaacc agcctgcccc agcctttccc
     1741 cagtcgcctg ggaatatctt cggaaaggcc tgccggaccg ttcaggcccc cgcaatcctt
     1801 tcctgttctt tcaatgaact ggtcacacca tacatcagaa cacactcctg tcaacaaaaa
     1861 aaagttgcaa aatattgata gtaaacatgt tgtgaatatg ctgtgaataa ccttcccgag
     1921 cccctcgcgc ttcgtcggtt ctgccaacca agacggattt cctcgattcc gaataccgac
     1981 aacctacaga tgcaacgcga ctggagttgg gcaggcgcgc tggacattca atgctgagaa
     2041 tgacatgttg ggccaggagc gttctgatgg cggaccgggg cccagtttgc aggtgagacg
     2101 tgcggtgcga actcacgccc tgcgctgacg ccattgaagc tccgattctg gaaggcaggc
     2161 cgctgtgccg cgaaagcgca tcgaatggat ttccgcctca aaccgaccag atgcgcgcag
     2221 gcgcgggcgt atgccagcgg caactggtcg gtttgaggcg gagcttcgtt ggaagccctg
     2281 gacatttagc acccgactgt gagcgcatga gttgcgattc a
//
LOCUS       DAOQRO010000424         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_57150_length_2309_cov_4.699201, whole genome shotgun sequence.
ACCESSION   DAOQRO010000424 DAOQRO010000000
VERSION     DAOQRO010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57150_length_2309_cov_4.699201"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2309
                     /locus_tag="PKK20_12610"
     CDS             <1..>2309
                     /locus_tag="PKK20_12610"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNV00773.1"
                     /translation="LEWTGVLDNGDGGSANRLHIMMQGPGECLVDNVEVVPSTGGNRI
                     ANPGFESGLTGWVIQGNHRRSEVQASGGIDNSRCLHVRATGRGDTACNRIYIPISPAL
                     AQNTTVTLRARVRWLKGWPEFMLRTRGSYLEAAGRMALPSDLGTPGARNSRAVANAGP
                     AIVEVVHTPVLPAANQAVVITARLSDPDGLGTVRVRYRIDPGGTTSDLAMKDDGTAGD
                     AIAGDGVYSARLSGRPSGTLVAFHVEAADNAQPSATARFPADAPDRECLVRWGETQPV
                     GNLGVYRLWQRRADYDWLRTREPLANDNVDCTFVYDDGRVVYNVEMRGKGSPWHGGSV
                     GSDYIFAFPRDDRFLGARDVAVVTVGNLGNDDSAQREQAAFWIGRQMGIPTLHRRHVL
                     FFENGARKQTVYEDTEEPSGRYVDRWWPAGEDGDLYKVEDWFEFNDTGNSFTFSRDAT
                     LEKFTTTGGAYKLARYRWAWRKRAVTDSANNYTNLFNLVTALNLAGSALVPQVENQVD
                     VENWMRVFALQHIVGNWDAYGYNRGKNAYIYKPVNGRFGMIPWDIDMVLGSGSDGPTT
                     DIFGANDPTVTRLWNTPAFRRVYLRAYWDAVAGPLQNARFDPVVDGRFAALSANGVSV
                     ANPRAIKSWVAQRRAYLANRVAGMDVKEFAITSNGGNDWSTGQLQVTLSGTAPIAIKT
                     IELNGAVMPVTWTSATSWSAQIALSGRTNVLNLVGYDSRGAPVAGALDTVRIIYTGPE
                     LPAPADQVVINEIMYHPLAPNAAFIELYN"
BASE COUNT          423 a          716 c          789 g          381 t
ORIGIN      
        1 cacttgaatg gacgggtgtg ctggacaacg gcgatggcgg cagtgccaac cgtttgcaca
       61 tcatgatgca ggggcccggc gaatgcctgg tcgacaacgt cgaggtcgtg cccagcacgg
      121 gcgggaaccg gattgccaac ccgggcttcg aatcgggact caccggctgg gtgattcagg
      181 gaaaccatcg gcgcagcgag gtccaggcca gcggcgggat cgacaacagc cgatgcctgc
      241 atgtgcgggc tacgggtcgt ggcgataccg cgtgcaaccg catttacatt ccgatctcgc
      301 cagccttggc tcagaacacg accgtcaccc tgcgcgcccg agtccgatgg ctcaaaggat
      361 ggcctgagtt catgctgcgc acgcgcggca gctacctcga agccgcgggg cgtatggcgt
      421 tgccttccga cttgggcacg ccgggcgccc gcaacagccg cgcagtggcg aatgcggggc
      481 cggcgattgt cgaggtcgtt cataccccgg tcctgcccgc cgccaaccag gccgtcgtga
      541 tcactgcccg gctgagcgat ccggatggtc tgggaacggt ccgcgtgcgg taccgcatcg
      601 acccaggcgg cacgacgagc gacctggcca tgaaggacga cgggacggcg ggcgatgcga
      661 ttgcgggcga cggcgtttac tcggcgcggc tcagcgggag gccctcgggc acgctggtgg
      721 ctttccatgt cgaggcggcg gataacgcgc agccctcggc aacggcgcgt tttcccgcgg
      781 acgcaccgga tcgggagtgt cttgtgcggt ggggcgagac ccagccggtg ggcaatctgg
      841 gagtctatcg tctgtggcag cggcgcgcgg attatgactg gctgcggacg cgtgaaccgc
      901 tggccaacga caatgtcgat tgcacgttcg tctatgacga cggacgggtg gtgtacaacg
      961 tcgagatgcg cggcaaaggg agcccgtggc atggcggcag cgtgggcagt gactacatct
     1021 tcgcctttcc cagagacgac cggttcctgg gggcgcggga tgtggccgta gtgaccgtgg
     1081 gcaatttggg caacgacgac agcgcccaac gcgagcaggc ggcgttctgg atcgggcggc
     1141 aaatgggcat tccgaccctt catcgccggc acgtgctatt cttcgagaat ggcgcgcgca
     1201 agcagactgt gtacgaggac accgaggaac cgagcggtcg ctatgtggat cgttggtggc
     1261 cggcgggcga ggacggcgac ctctacaagg tcgaggactg gttcgagttc aacgacacgg
     1321 gcaacagttt caccttcagc cgggatgcca cgctcgaaaa gttcacgacc acgggcggcg
     1381 cgtacaagct ggcgcgctac cgctgggcat ggcggaagcg ggcggtgacc gattccgcca
     1441 acaactacac caacctgttc aaccttgtca cagcgctgaa cctggcggga tcggccctgg
     1501 tgccacaggt cgagaaccag gtcgacgtcg agaattggat gcgcgtcttc gctctccagc
     1561 acattgtggg aaactgggac gcttacggct acaatcgcgg caagaacgcc tacatctaca
     1621 aaccggtcaa cggacgcttc ggaatgattc cctgggacat cgacatggtg ctcggctccg
     1681 ggagtgacgg cccgacgacg gacatctttg gcgcgaacga tccgaccgtg accaggctct
     1741 ggaacacccc ggcgtttcgt cgcgtctatt tgcgggccta ttgggacgcc gtcgccggcc
     1801 cgctccagaa cgcgcggttc gatcccgtcg tggacgggcg gtttgccgcg ctgagcgcca
     1861 acggcgtcag cgttgccaac ccccgtgcga tcaagagttg ggtggcccaa cgccgggcgt
     1921 acctggccaa ccgggtggcc gggatggacg tgaaggagtt cgccatcacc agcaacggcg
     1981 gcaatgactg gagcacgggc cagttgcagg tgacgctcag cggaacggcg cccatcgcga
     2041 tcaagacgat cgagttgaac ggcgccgtga tgccggtgac gtggaccagc gcgaccagtt
     2101 ggtccgccca gatcgccttg agcgggcgca ccaacgtcct gaaccttgtc ggctatgatt
     2161 cgcggggcgc gcccgtggcc ggcgctctcg acaccgtccg catcatctac actggcccgg
     2221 aactgccggc gcccgccgat caagttgtga tcaacgagat catgtaccat cccctggcgc
     2281 cgaacgccgc gttcatcgaa ctctacaac
//
LOCUS       DAOQRO010000425         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_58196_length_2283_cov_4.596499, whole genome shotgun sequence.
ACCESSION   DAOQRO010000425 DAOQRO010000000
VERSION     DAOQRO010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58196_length_2283_cov_4.596499"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKK20_12615"
     CDS             <1..388
                     /locus_tag="PKK20_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00774.1"
                     /translation="EELLSIDVQYDKTQLTTADLLTCTVRVANNQPGKAKMVVVDVGI
                     PPGFEVVRDDLDQLVGEAIQKYQVAGRQIIFYLGELDHAAPVSLVYRLAAQYPIRAKT
                     PRSVVYEYYNPDVRGVAEPVEIAVTN"
     gene            395..1306
                     /locus_tag="PKK20_12620"
     CDS             395..1306
                     /locus_tag="PKK20_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00775.1"
                     /translation="MNASILRRLFPARDSRGWAHAGAALMLAAWVGQAVTISVEDVSG
                     VPGAQLVARIWVDQASGIAGGTFRMIVPDFAGIEAPSLASDTAGFVVAARVGNRRLDV
                     SMARAAGMRAGQAILFTVPIRIGRGAPEGTFAILWEEARLFDETTAPIQPIATSGAIT
                     VLPPLPDLDQDGLPDSWEMERFGHLNSGAEDDPDADGASNYSEYLAGTDPASAGSLFV
                     VTALAVSGQSGESFVALDWEGQPGRNYEVFWSPGPLGPGARWLPVYHPVYRIDGNRYR
                     WIDDGTRTGWFPFVDAERYYQVRVELP"
     gene            1664..>2283
                     /locus_tag="PKK20_12625"
     CDS             1664..>2283
                     /locus_tag="PKK20_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNV00776.1"
                     /translation="MTGLILLVGGYFFYGRVVAATVRPDPNRLTPAIALADGMDYVPM
                     PTWRVYLIQLLNIAGLGPVFGPILGALWGPQVFLWVVLGCIFGGAVHDLLTGTMSIRN
                     RGAGLPDLIGHYMGNAARQFSTFFILLLMVLVGTVFVKGPALLLVSVLPSETVGGWFG
                     EAAKEWLSQTYRGHGMWLWIVMVSIYVYYLAATLLPIDKIIGRFYP"
BASE COUNT          362 a          689 c          756 g          476 t
ORIGIN      
        1 cgaagaactc ctgtcgatcg acgtccagta tgacaagacg caactgacca ccgccgatct
       61 gctgacctgc acggtccggg tggcgaacaa ccagcctgga aaagccaaaa tggttgtcgt
      121 ggatgtgggg attccgcccg gattcgaagt cgttcgggac gatttggatc aactggtggg
      181 cgaggcgatc cagaaatacc aggtggccgg tcgccagatc atcttctacc tcggtgaact
      241 cgatcacgcc gctccggtca gccttgtgta tcgcctggcg gcgcagtacc ccatccgcgc
      301 caagacaccg cgatcggtgg tgtacgagta ttacaatccg gacgtgcgtg gggtggcgga
      361 gccggttgaa atcgccgtaa ccaattagcg tgctatgaat gcctcgattc tccgaagatt
      421 gttccctgct cgcgacagtc gcggttgggc gcacgcaggg gccgccctga tgctcgccgc
      481 ctgggtgggc caggccgtca caatcagcgt cgaagacgtt tccggtgttc cgggcgccca
      541 actcgttgcc cgcatctggg tcgatcaggc gtcgggaatc gccggcggga cctttcgcat
      601 gatcgtgcct gactttgccg gcatcgaggc gccgagcctt gcatcggata ccgccggctt
      661 cgtggtcgct gcccgggtgg gcaaccggcg tctggatgtc tcgatggccc gcgccgcggg
      721 catgcgggcc ggacaggcga tcctgttcac cgtcccgatc cgcattgggc gcggcgcgcc
      781 tgaaggcacg ttcgcgattc tgtgggagga ggcgcggctc ttcgacgaga cgaccgctcc
      841 aatccagccg atcgcgacgt ccggcgccat cacggtcctg ccgcccctgc ctgatctgga
      901 tcaggacggc ctcccggatt catgggaaat ggagcggttc ggtcatttga acagcggggc
      961 cgaagatgac cccgacgctg acggcgcctc gaactactcg gagtatctcg ccgggaccga
     1021 tccagcttct gccggctccc tatttgtcgt gacggccctg gcggtgtccg gtcagagcgg
     1081 tgaatccttc gtcgccctcg attgggaggg tcaacccggg cggaactacg aagtcttctg
     1141 gagccccggt cctctgggtc cgggcgctcg ctggctgcct gtctatcacc cggtctatcg
     1201 aatcgatggc aatcgttatc ggtggattga cgacgggacg cgcaccggct ggtttccgtt
     1261 tgtcgacgcg gagcgttact accaggtgcg cgtcgagctc ccctgaatca acgggactca
     1321 tgccgggacc gctcgggacg cggcgtgccg gagcaatgtg cgttcccagg cggcagcggc
     1381 agtcgaccgc ctccaacctc gcccgactgg cgcattcgcg ttctgctgtt accgacccgt
     1441 gcattctgcg cgtgacagcc cggtcggcat ctggcagcgt cgccctatga taggcggagc
     1501 tgaagggcga tttggcccgc gtgcgatttc cagactcatg ccaatccatc ggcacgatgc
     1561 gaagcgcagt tcatcgaaga cgcagcggcg gtccgtggcc agagcccggc gcgcctgtgt
     1621 cgactgtgtc agcaaataac cctcgactgg catgatcgcg tttgtgacag gactgatctt
     1681 gctggtggga gggtatttct tttacggacg agtcgtcgct gccacagtac gtcccgatcc
     1741 caatcggttg acgcctgcga tcgcgcttgc cgacgggatg gactacgtgc cgatgcccac
     1801 ctggcgggtc tacctgatcc aactgctgaa cattgccggc ctgggtccgg tttttgggcc
     1861 gatcctgggg gccttgtggg ggcctcaagt gtttctctgg gtcgtgcttg gatgcatttt
     1921 cgggggcgca gtgcacgatc tgttgacggg gacgatgtcg attcggaacc gcggcgccgg
     1981 gttgcccgac ctgatcggcc actacatggg gaacgcggcg cggcagtttt cgaccttctt
     2041 cattctgctg ctgatggtgc ttgtggggac cgtgtttgtg aagggaccgg cgcttctgct
     2101 ggtcagcgtg ttgccgtccg agaccgtggg cgggtggttc ggggaggccg caaaggagtg
     2161 gctctcccag acttaccggg ggcatgggat gtggttgtgg atcgtgatgg tgtcgatcta
     2221 cgtgtactac ctggcggcga cgctcctccc gatcgacaag atcatcggac ggttctaccc
     2281 gtt
//
LOCUS       DAOQRO010000426         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_58859_length_2266_cov_7.015830, whole genome shotgun sequence.
ACCESSION   DAOQRO010000426 DAOQRO010000000
VERSION     DAOQRO010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58859_length_2266_cov_7.015830"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..>2266
                     /locus_tag="PKK20_12630"
     CDS             319..>2266
                     /locus_tag="PKK20_12630"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNV00777.1"
                     /translation="MNSLGLPRPAYNRLLSASHAAAIAAVIAVMAHTTGSRADDSGGQ
                     ADTFDAPGNCNPIVPGYFADPTIKQFGDTFYLYATTDGNGGGRGPATVWVSRDFVHWV
                     LVPMNWPATPHYWAPDVVRRPDGRYYLFYNQPCNTFAGVSDSPTGPWTPLTPGDGLVI
                     PDRLVKDVITLDTQFFEDKDGTLYGYWGTWGIFANSGCGYGVFNPDMKSFARLGMIPN
                     TQAKDFFEAPFMLERNGVYYFTYSSGSCHDASYRVQYAVSNKPDGEFKMGPNNPILAT
                     TPDGKVHGPGHHSILRCGNDDFIVYHRHDIPVTPNGMHRQTCADKLVFASDGVIEKVV
                     PTHRGIGFLGPQARGPNLALAKTVTASSHYLDTLRKHSYKPEYAVDDNNATLWRPADN
                     RMGHWLTVDLGSPQRIRRTETQFEYATWFYQYLIETSLDGQTWTTFADRRQNTRWGSP
                     MVDDGDVQTRYLRLTVTGTEFLGLFGAIWNFKAYADAPADSLLTLANQAFENLPSSGR
                     QGRAREAVQSAASRVQSEVSSGRDSQSASIAPIIHLDVADLQLGAAVAAWTNHGTLGG
                     TFTSRDAQPAVDLASGRKAVRFAGKELLTASFPSPRALSGNSSFTVAAWVNNPEIAES
                     ECFLSWAGRGGPDAATAQFGYGTQP"
BASE COUNT          426 a          852 c          601 g          387 t
ORIGIN      
        1 ctcccgtccg cggctacgaa acggccgcgg gacggctgcg cctccgtagc cgccgacgtg
       61 agacggcgct gattcctgct tttgacggag cgagtcagcg cggactcacg tccgcggcta
      121 cggaaccacg gttcatggaa agcattgaca ttcaacggtt taatgcgcat tggacccatg
      181 aaccggggaa cgttcaacgc ccaacttcca acgctcaacg ttcaacgaag cggttcgtta
      241 aaagttggat gttgaacgtt gcgcgttgga cgttcggatt gcagctttgt ccaagtcacg
      301 atccaaaccc ttgaccatat gaacagcctc ggccttcccc gccctgcata caaccgcctc
      361 ctgtccgcca gccacgctgc cgccatcgcc gccgtcatcg ccgttatggc gcacacgacc
      421 gggtcgcgcg ccgatgattc cgggggccaa gccgacacct tcgacgcacc cggcaactgc
      481 aatccgatcg tccccggcta ctttgccgat ccgaccatca aacagttcgg cgacaccttc
      541 tacctctacg ccacaaccga cggcaacggc ggcggacgcg gccccgccac ggtttgggtg
      601 tcccgggact tcgtgcattg ggtcctggtg ccgatgaact ggcccgccac gccccattac
      661 tgggcgcctg atgtcgtccg gcgccccgat ggccgctact acctctttta caaccagccc
      721 tgcaacacct tcgccggcgt gtctgactcg cccaccggcc cctggacgcc gctcacaccc
      781 ggggatggac tggtgatccc ggaccgcctg gtcaaagatg tgatcaccct cgacactcag
      841 ttcttcgagg acaaggacgg cacgctctac gggtactggg gcacctgggg catcttcgcc
      901 aacagcggct gcggctatgg cgtgtttaac ccggacatga agtccttcgc ccgcctcggc
      961 atgatcccga acacccaggc caaggatttc ttcgaagccc ccttcatgct cgaacgcaac
     1021 ggcgtctact acttcaccta ttcctccggc tcctgccacg acgccagcta ccgcgtccaa
     1081 tacgccgtca gtaacaaacc cgacggcgaa ttcaaaatgg gcccgaacaa ccccatcctc
     1141 gccaccacgc ccgacggcaa ggtccacggc cccggccatc attcgatcct cagatgcggc
     1201 aacgatgact tcatcgtcta tcatcgacac gacatcccgg tgacacccaa cggcatgcat
     1261 cgccagacct gcgcagacaa actggtgttc gcctcggacg gcgtcatcga gaaggtcgtg
     1321 ccaacccatc gcggcatcgg ttttctcgga ccgcaggccc gtggccccaa cctggccctt
     1381 gcgaaaaccg tcaccgcctc gtcgcattac ctcgatacgc tccgcaaaca ctcgtacaaa
     1441 cccgagtacg ccgtcgatga caacaatgcc accctctggc gccccgccga caaccggatg
     1501 gggcactggc tcaccgtcga cctgggcagc ccccaacgca tccgccgcac cgagacgcaa
     1561 ttcgagtacg ccacctggtt ctaccagtac ctcatcgaga cttcgctcga tggccagacc
     1621 tggaccacct tcgccgaccg ccgccaaaac acccgttggg gcagccccat ggtggacgac
     1681 ggcgatgtcc agacccgtta cctgcgcctc acggtcaccg gcaccgagtt cctcggcctc
     1741 ttcggcgcca tctggaattt caaagcttat gccgatgcgc ccgcggattc cctcctgacc
     1801 ctcgccaacc aggcgttcga gaacctgcca tcttcgggcc gtcagggacg cgccagggag
     1861 gcagtgcaga gtgcagcgtc gcgagtccag agtgaggtca gcagcggccg cgattcccag
     1921 tccgcgtcca ttgctcccat catccacctc gatgtcgccg accttcagct cggcgccgcc
     1981 gtcgccgcct ggaccaacca cggcaccctc ggcggcacgt tcaccagccg cgacgcccag
     2041 cccgctgtcg atttggcgtc cggacgcaaa gccgtgcgct tcgccggcaa ggaactcctc
     2101 accgcgtcgt tcccatcccc gcgcgccctc tccggcaata gcagcttcac cgtcgccgcc
     2161 tgggtcaaca accccgagat cgccgaaagc gagtgcttcc tcagttgggc cggacgcggc
     2221 gggccggacg ccgccaccgc ccagttcggc tacggcacgc agcccg
//
LOCUS       DAOQRO010000427         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_60378_length_2230_cov_3.909425, whole genome shotgun sequence.
ACCESSION   DAOQRO010000427 DAOQRO010000000
VERSION     DAOQRO010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60378_length_2230_cov_3.909425"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..332
                     /locus_tag="PKK20_12635"
     CDS             168..332
                     /locus_tag="PKK20_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00778.1"
                     /translation="MLLERLCGKRYDLADLAQLAEFLTARPGPVEACGRVVKTAVRLA
                     AEVVLDEPPR"
     gene            complement(329..1228)
                     /locus_tag="PKK20_12640"
     CDS             complement(329..1228)
                     /locus_tag="PKK20_12640"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNV00779.1"
                     /translation="MKTSLIIGTGCAILMAIGAQDIVSPVHAQGNAKTPPKLTPATIE
                     RIEAAAPAEAVAKPRAARRILVFWRCEGFFHGEGIAGGNHALAHMGRRTGAYTADFSD
                     DYSVFDPNQLAKYDAIVLNNTTHLKFPDAVKKQALLDFVQKGKGIVGIHAATDCFYDW
                     PEAAAMLGGLFDGHPWGAGGTWAFKIEEPTHTLNRAWGGKGFKLQDEIYQFTAPYTRA
                     DRRVLVSLDLSDPATAAVKNGVKRTDGDFAVAWIKKHGQGRVFYCSLGHAANVFEDAG
                     VLRFYLDGIQYALGDLEADASPR"
     gene            complement(1297..>2230)
                     /locus_tag="PKK20_12645"
     CDS             complement(1297..>2230)
                     /locus_tag="PKK20_12645"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNV00780.1"
                     /translation="GRFYPFFAAALLVMVVGLGIAILRGQIVVPPFTLKNLHPEQLPA
                     WPLIFITVSCGAVSGFHATQSPLMARCLKSERHLRLVFYGAMIAEGLIALVWASVAQG
                     FYGSHDDLAAVLLKPGGGGPGAVVHDACTATMGVFGGGLAILGVVVLPITSGDTAFRV
                     SRLILADYFHVSQVRFLNRSLLITPLFVISFALNFVDFSVIWRYFGWANQALAAVALW
                     GGTIFLARRRTRWWLAFVPAVFMTVVTATYILVEDVGFRLNPTLGAWLGLLIGAAAAA
                     WFLVRLPKLPVEPEEPAITARPTAAERSTDSLAC"
BASE COUNT          450 a          706 c          679 g          395 t
ORIGIN      
        1 ggaagggttc ggcccggagg gcggcgccat cgtgaaagcc ctcactccgc tgcccggaat
       61 tgccgagcgc ggcgagacgt cggcgcggtg attggcgcgc ttcgcttggg cccgctcggg
      121 ccttctgcgc ccgtttcgaa catgccctgg gcagcaccca gtgcgttgtg ttgctcgaac
      181 ggctatgcgg gaaacgctac gatctcgccg atctggcgca actggctgag ttcctgactg
      241 cccgcccagg gcccgtcgag gcctgcggcc gcgtcgtcaa gacggcggtc cggctcgcgg
      301 cggaggtggt tttggacgag cctcctcgtt agcgggggct cgcatccgct tccaggtcgc
      361 ccaacgcata ctggatgccg tccagataaa agcggagaac gccagcatcc tcaaacacgt
      421 tggcggcgtg tccgagactg caataaaaga ctcgcccctg gccgtgtttc ttgatccagg
      481 cgacggcgaa atcgccatcc gtccgtttga cgccgttttt caccgcggct gtagccggat
      541 ccgagagatc cagactgacc agcacgcggc gatcggcgcg tgtgtagggc gctgtgaatt
      601 ggtagatctc gtcttgcagc ttgaatcctt tgccgcccca ggcccgattc agagtatgcg
      661 tcggctcctc gatcttgaac gcccaggtgc cgcccgctcc ccacgggtgc ccgtcaaaaa
      721 gaccgccgag catcgccgcg gcctccggcc aatcgtagaa gcaatccgtc gcggcatgga
      781 tgccgacaat tcccttgccc ttttggacga aatcgagcag ggcctgcttc ttgacggcat
      841 ccggaaactt caggtgtgtc gtgttgttca ggacaatcgc atcgtacttc gccaattgat
      901 tcggatcgaa cacgctgtag tcgtcactga aatcagccgt gtacgcgccc gtgcggcgcc
      961 ccatgtgggc cagagcatga ttgccgccgg caatgccctc gccgtggaag aaaccttcgc
     1021 atcgccagaa caccagaatc cgccgcgccg cccggggctt ggccacagcc tccgcgggag
     1081 cggctgcttc aatccgctcg attgttgccg gcgtcagttt cggcggcgtc ttggcgttgc
     1141 cttgtgcatg cacggggctg acgatgtcct gagccccgat ggccatgagg atggcacatc
     1201 ccgttccgat gatgagtgat gttttcattc gattcgagtt ttggtgatgg attgagtgag
     1261 tggaccagtg aaagagcgtt tcttagtctg cgccgattag caggcaagcg aatccgtgga
     1321 ccgttcggca gccgtcggtc gggctgttat cgccggttct tccggctcga ccggcagctt
     1381 cggcaatctc accagaaacc atgccgccgc cgcggctccg atgagcagcc caagccaggc
     1441 gcccagggtc gggttcagac gaaacccgac gtcctcgacc agaatgtaag tcgccgtcac
     1501 cacggtcatg aatacggcgg gcacgaaggc cagccaccat cgagtcctgc gccttgccag
     1561 gaagatggtc ccgccccaca aggcaacggc tgccagggcc tgattggccc agccaaagta
     1621 cctccaaatc acgctgaaat cgacgaagtt caaggcgaaa gaaatcacaa acagaggcgt
     1681 gatgagcaaa gagcggttga ggaagcgcac ctgggacacg tggaaataat cggccaggat
     1741 caagcggctg actcggaagg ccgtgtcgcc agatgtaatg ggcaacacga ccacgcccag
     1801 gatcgccaac ccaccgccaa aaacgcccat tgttgcagta caggcatcat ggaccactgc
     1861 gccaggtccg cccccgccgg gcttgagaag aaccgcggcc aggtcgtcgt ggctgccgta
     1921 gaaaccctgg gcgaccgacg cccagaccag cgcgatcaac ccctcggcga tcatcgcgcc
     1981 gtaaaacacc agacgcagat gtctctcgct cttgagacac cgggccatca ggggactctg
     2041 cgtggcgtga aaaccgctga ccgcgccgca actgacagtg atgaagatca aaggccaggc
     2101 aggcagttgc tcgggatgca gattcttgag agtaaagggc ggcaccacaa tctgtccccg
     2161 caagatcgca atgcccaagc cgaccaccat gaccagcaag gccgccgcga agaacgggta
     2221 gaaccgtccg
//
LOCUS       DAOQRO010000428         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_65894_length_2112_cov_4.015070, whole genome shotgun sequence.
ACCESSION   DAOQRO010000428 DAOQRO010000000
VERSION     DAOQRO010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65894_length_2112_cov_4.015070"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1089
                     /locus_tag="PKK20_12650"
     CDS             232..1089
                     /locus_tag="PKK20_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV00781.1"
                     /translation="MKSPCVRLTRSGFTLIELLVVIAIIAILAGMLLPALSKAKAKGQ
                     GISCMSNTKQLALGFLLYADDHDETLLGCLDGLPGRVNWVQGDLQGWDANAADNRFLT
                     NSPLFNYVGRSLSIFKCPADKAMVRVGGRMVPRIRSNSMSQVFAWGDWLDGSGTGRNQ
                     TRWRTYSKASHIVTPTKTFVFVDEHPNSINDAAFATQCTGADTMNTARIIDIPANYHN
                     GACGFSFADGHSEIKRWVSDRSGHDTGRGLRNMPVYFTGARNLALNFAAREAWVDAKW
                     MADNTTEPR"
     gene            complement(1105..>2112)
                     /locus_tag="PKK20_12655"
     CDS             complement(1105..>2112)
                     /locus_tag="PKK20_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV00782.1"
                     /translation="LQTIDAVQAGAHVYVEKPICHTILEGRAMVQAARNTGKTVQVGT
                     HRRVSPHNVSARAFIRSGKLGKVGMIRAFVHYGGGPERPRRNIEPPPELDWEMWCGPA
                     PMRPFNGDPDNPWSGGIHPRGFRNYLDYANGTLGDWGIHWMDQVLWITGEKWPRRISS
                     YGGRPIKGPPVSTAEEQTTDAPDHQVAIFDFEDFTLSWEHRQFAGNHAEKGESVGCYF
                     YGTEGTLHLGWKEGWVFYPVDARKPVVRESPRLHAPDDQNIKEVWADLLQSIKTGARP
                     VCDVEEVGRSTNLSLLGMLSLKLGRSVQWDGEKTEAIGDPEANSLLRREYRRGWKYPA
                     V"
BASE COUNT          392 a          696 c          627 g          397 t
ORIGIN      
        1 cgggcggatc ggcactgaac agttcgtggt ttgtcgagat catcgcacgc ctactgcgca
       61 ccaaaccacg ccaattgatg agcactattt ccaacctcaa caacagcaac ccttcaggaa
      121 tttgaatgaa atgaccgtgg aggtggccac agggctgggc ggttcggtct tgaccgcccc
      181 gtgcgacagg ctagcgttgg ggtgttgccc gaaagccaat ttgacgagct tatgaaatcg
      241 ccttgtgttc gcctgacccg ctcaggcttt actctgattg aactcctggt ggtcatcgcc
      301 atcatcgcga tcctcgccgg tatgctgctc cctgccctct cgaaggccaa ggcgaagggc
      361 cagggcatct catgcatgag caacaccaaa caactcgcgt tgggctttct gctctatgcg
      421 gacgaccacg acgaaacgtt gctggggtgc ctggatgggc tgcccgggcg cgtgaactgg
      481 gtgcagggtg atctgcaagg ctgggacgcc aacgccgccg acaacaggtt tctcaccaac
      541 agcccccttt tcaactacgt cggccgcagc ctctccattt tcaagtgtcc tgccgacaag
      601 gccatggtgc gggtgggcgg acggatggtg ccccgcatcc ggagcaactc gatgagccag
      661 gtgttcgcct ggggcgattg gctggacgga tcgggcaccg gccgcaatca aacccgctgg
      721 cgcacctact cgaaggcctc ccacatcgtc accccgacca agacattcgt ctttgtggat
      781 gaacatccca acagcatcaa cgacgccgca ttcgccaccc aatgcaccgg ggccgacacc
      841 atgaacaccg cccgcatcat cgacatcccc gccaactacc acaacggcgc ctgcggattc
      901 tcctttgccg acggccactc ggaaatcaag aggtgggtca gtgaccgctc ggggcacgac
      961 accggccgcg gcctgagaaa catgccggtc tatttcaccg gggcccgcaa cctggctctc
     1021 aacttcgccg cgcgcgaggc ctgggtcgac gcgaagtgga tggccgacaa caccaccgag
     1081 ccgcgttaga acagcctgcg cctctcagac cgcgggatac ttccagcctc gccggtattc
     1141 gcggcggagc aggctgttgg cttcggggtc gccaatcgcc tcggtcttct cgccatccca
     1201 ctgcacgctg cgtcccagtt tgagactcaa catgccaagc aggctcaggt tggtcgagcg
     1261 tccgacttcc tcgacgtcgc agacgggcct cgcaccggtc ttgatggatt ggaggagatc
     1321 ggcccaaacc tccttgatgt tctggtcgtc cggagcatgc aaccgcgggc tctcccgaac
     1381 gaccggtttg cgcgcgtcga cggggtaaaa gacccagccc tccttccacc ccaagtggag
     1441 cgtcccctcg gtgccgtaga agtagcagcc gacactctct cctttctccg cgtggttgcc
     1501 ggcaaattgg cgatgctccc aggagagcgt gaaatcctcg aaatcgaaga tggccacctg
     1561 atgatccggc gcatcggtgg tctgttcctc ggccgtcgag accggagggc ctttgatcgg
     1621 acgcccgcca taggacgaaa tcctgcgggg ccacttctcg ccggtgatcc agagcacctg
     1681 gtccatccag tgaatgcccc agtctcccag ggtgccgttg gcatagtcca ggtagttgcg
     1741 gaatccccgc gggtgaatgc ctccgctcca tggattgtcc ggatcgccgt tgaacggccg
     1801 catcggcgcc gggccgcacc acatctccca atccagttca ggcggcggct cgatgttcct
     1861 gcgcgggcgc tcgggcccgc ctccgtaatg gacgaaggcg cggatcatgc ccaccttccc
     1921 caatttgccg gagcggatga aggcgcgcgc cgagacattg tgcggcgaga cccgcctgtg
     1981 ggtgccgacc tggaccgtct tgccggtatt gcgcgcggcc tgaaccatcg cgcgtccttc
     2041 gagaatggta tggcaaatgg gcttctcgac gtacacgtgg gcgccggcct ggacggcgtc
     2101 aatcgtctgg ag
//
LOCUS       DAOQRO010000429         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_66224_length_2106_cov_4.728425, whole genome shotgun sequence.
ACCESSION   DAOQRO010000429 DAOQRO010000000
VERSION     DAOQRO010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66224_length_2106_cov_4.728425"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..78
                     /gene="ffs"
                     /locus_tag="PKK20_12660"
     ncRNA           <1..78
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK20_12660"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            164..1975
                     /gene="dnaX"
                     /locus_tag="PKK20_12665"
     CDS             164..1975
                     /gene="dnaX"
                     /locus_tag="PKK20_12665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNV00783.1"
                     /translation="MSYQVIARKYRPQRFADVVGQEHVTQTLANAVRQNRIAHAYLFC
                     GPRGTGKTTIARIFAKCLNCTNGPSVDFADEDSKCIEIAEGRSIDVLEIDGASNNGVE
                     QVRELRETVKYAPASSTFKIYIIDEVHMLSAAAFNALLKTLEEPPAHVKFMFATTDPQ
                     KVLPTILSRCQRFDLRRIPTALIVRHLEKIARAEGVQVEGAALYAIGRGADGGMRDAE
                     STLDQLISFCGNSIGEADVLTMFGLTAQAQILALANAMLGGQADVVLRELDALAQAGK
                     DLGRLLTDLLGHFRNLLVYQVSRGDLDLLEVSEAEAAALKEQAPRIDTEPLTRMLETL
                     TDTEARLGDAVSKKIALEVALLKTIQARSALSLDAVLQRLNQLRQESAAQDDPAPVGR
                     AETAAPPRSDVASPAPESIAAPAASRAFALSANEDAPSASASADDDVARPAAVGDDLE
                     TLWNRLLDAVGRVSPFTRSYLLKAFPASFERGVLVIGFDPEFADYLPLVDNARNQALL
                     QTKLREMGLAEAQVRFAKLSAPPPQTRKSSPAAPDPALAAPSESPATLAAPKASSAQP
                     EARETKPARRNPSEFKDDPLIKQAIEIFRGQIVEVRS"
BASE COUNT          394 a          690 c          651 g          371 t
ORIGIN      
        1 tggacttacg aaccccgcca gggccggaag gcagcaacgg cagtctgctc cgcatctgcc
       61 gtggtcaccc tggcattttc attttagtgc ggtgcaggtg gattccgaca gacaggcgcg
      121 ggggggaagt gtccgcggat taccgccgcc ccgaggcatt cgaatgagct accaggtcat
      181 agcccgtaag taccgtccgc agcgatttgc ggatgttgtg gggcaggagc atgtgacgca
      241 aacgctcgcc aatgcggttc ggcagaaccg gatcgcgcat gcgtatttgt tttgcgggcc
      301 gcgcggcacg ggcaagacca ccatcgcccg cattttcgcc aagtgcctca actgcacgaa
      361 cgggccgtcc gttgactttg cggatgagga ttcgaagtgc atcgagatcg ccgagggacg
      421 ttcgattgac gtgctcgaga ttgacggcgc cagcaacaac ggagtggaac aggtgcgcga
      481 acttcgcgag acggtcaagt acgccccggc ctcgtccaca ttcaaaatct acatcatcga
      541 cgaggtgcac atgctctcgg ccgcggcgtt caacgccctg ctcaagaccc tcgaagagcc
      601 gccggcgcac gtgaagttca tgttcgccac cacggacccg caaaaggtcc tcccgacgat
      661 cctgtcacga tgccagcgat tcgatctgcg ccgcattccc accgccctga tcgtccggca
      721 tctcgagaaa atcgcccgcg ctgaaggcgt gcaggtcgag ggtgcggccc tgtacgccat
      781 cgggcgcggc gccgatggcg ggatgcgcga tgcggagtcg acgctcgatc agttgatcag
      841 tttttgcggc aacagcatcg gggaggcgga tgtgcttacg atgttcggtc tgacagccca
      901 ggcccagatt ctggccctgg ccaatgccat gctcggcgga caggctgatg ttgtgttgcg
      961 cgaactcgat gcgctggccc aagccggcaa ggatctgggc cgtttgctga ccgaccttct
     1021 gggccatttc cgcaacttgc tggtctacca ggtgtcgcgt ggggacctcg atctgttgga
     1081 ggtgtcggag gccgaggccg cggccctcaa ggagcaggca ccccggattg acaccgagcc
     1141 gctcacgcgc atgctcgaaa ccctgaccga caccgaggcg cggctcggcg atgcggtgtc
     1201 caagaagatc gccctcgaag tggctctgct caaaaccatc caggcccggt ccgctctgag
     1261 cctcgatgca gtccttcagc gcctcaacca actgcgtcag gaaagcgccg cgcaagacga
     1321 ccctgccccg gttggacgcg cggaaacggc cgctccgcct cgatccgatg tcgcaagtcc
     1381 cgccccagag tcgattgccg cccccgcggc atcgcgcgcg ttcgcgctct cggccaatga
     1441 ggatgcaccg tcggcttcgg cgtcggctga cgatgacgtt gcgcggccgg cggctgtcgg
     1501 cgacgatctc gagacgctct ggaaccgcct tctcgatgcg gttgggcgtg tgagcccctt
     1561 tacgcgcagt tatctgctca aggccttccc ggcctcgttc gaacgcgggg tcctggtgat
     1621 cggtttcgac cccgagttcg ccgattacct gcctcttgtg gacaacgcaa ggaaccaggc
     1681 gctcttgcag accaagcttc gcgagatggg cctggcagag gctcaggtgc gctttgccaa
     1741 gctcagcgcg cctcctcccc aaacacgcaa atcgtctccc gcggcgcccg atcccgcgct
     1801 tgcggctccc tcagaatccc ctgcgactct tgccgctcct aaggcgtcca gcgcgcagcc
     1861 cgaagcccgg gagaccaaac ccgcacgacg gaatccatcc gaattcaagg acgatccgct
     1921 catcaaacag gcgatcgaga ttttcagagg acagatcgtt gaagtccggt cctgagactc
     1981 actccccgca tatggcgaac atcagcaaac tcatgaaaca ggccgcccgc ctgcagcagc
     2041 agatggaggc gactcaaggc caactggccg cacagaccgt cgaggcgacg agcggcggcg
     2101 gcgtgg
//
LOCUS       DAOQRO010000430         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_68382_length_2066_cov_3.694679, whole genome shotgun sequence.
ACCESSION   DAOQRO010000430 DAOQRO010000000
VERSION     DAOQRO010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68382_length_2066_cov_3.694679"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="tilS"
                     /locus_tag="PKK20_12670"
     CDS             complement(<1..1091)
                     /gene="tilS"
                     /locus_tag="PKK20_12670"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNV00784.1"
                     /translation="MTDLLAEVRTAILTRRLFERGERIVVAVSGGLDSMVLLRLLDEL
                     ASESAWSLIMAHFNHGLRGWEADRDEALVRDSAWRLGWPCVVGWADSALRKRQPGVSL
                     EMAARQARHRFLAEVAAQSGCSKVALGQHADDQIETVLLRLLRGAGGEGLAGIKWLGP
                     SPANPTIQLVRPLLGQTRATLRQWAGRADVSWREDRSNACADMSRNWLRIEIVPKLRR
                     RVGRGLAATVARTAALVGAEADLVNDLARRWRGARRRAAFGALHAAVQRRILVQQLIE
                     SGIEPEFELVEALRTEPGVSRTIGPGLTVRSDVRGHVEVERTAGVRFRGERHSVRLDG
                     DSGRIVFGGMTVVWRVVTRRGKDRYAILLP"
     gene            complement(1392..>2066)
                     /locus_tag="PKK20_12675"
     CDS             complement(1392..>2066)
                     /locus_tag="PKK20_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00785.1"
                     /translation="DVTRKTLEESAAMYLAQKALLKKYIANAITIDCLGGFYGGHIHA
                     YPCLGFHQLLNEGMVGACEADVRSTSAMIILTILSHGRPGYISDPVIDTSKREIVYAH
                     CVASNKPFGPRGKASPFDIMTHSEDRQGASVRSILPVGYLTTTVQFDQKRKEVVMHRA
                     RTVANDLDDRACRTKLCAEPIGDIEKLFRYWDQWGWHRVTCYGDFRNAVMELAATLEL
                     KVVEEA"
BASE COUNT          423 a          735 c          577 g          331 t
ORIGIN      
        1 ggcaacagta tcgcgtagcg gtccttgccg cgccgcgtca cgacccgcca taccaccgtc
       61 attccgccaa aaacaatcct ccccgaatcc ccgtctaatc gcaccgaatg ccgttccccg
      121 cgaaaccgca ccccggcagt tctttcgact tcaacatgcc cccggacatc gctccggact
      181 gtcaagcccg gaccgatcgt ccgggacacg cccggctccg ttcgaagagc ctcgaccaac
      241 tcgaactccg gctcgatgcc ggactcgatc aactgctgga ccaggatgcg gcgttggaca
      301 gccgcgtgca gcgcaccgaa cgccgcccgc cgccgcgctc cccgccacct gcgagccaga
      361 tcgttgacca gatcggcctc cgcgccaaca agcgccgccg ttctcgccac cgtagccgcc
      421 aacccgcgtc cgacacgccg cctcagcttc ggaacgatct cgatccgaag ccagttccga
      481 ctcatgtcgg cgcacgcatt gctccgatct tcgcgccagg acacatcagc acgcccggcc
      541 cactggcgca acgtcgcccg tgtttgcccc aggagagggc gaacaagctg gatcgtggga
      601 ttcgcgggcg acggcccgag ccacttgatg cccgccagcc cttccccacc cgccccccgc
      661 aacaggcgca acaggacggt ctcgatctgg tcgtcagcgt gctggccgag ggcgactttc
      721 gagcaacccg actgcgcagc cacctcggcc agaaaccggt gccgcgcctg gcgtgccgcc
      781 atttcgagcg agaccccagg ctgacgcttc cgcagcgcac tgtcggccca tcccaccaca
      841 cacggccatc ccagacgcca tgcgctgtcg cggaccagcg cctcgtcccg atccgcctcc
      901 cagccgcgga gcccgtgatt gaaatgggcc atgatcaacg accaagcgga ttcagaagcg
      961 agttcatcaa gcaaccgcaa caacaccatc gaatcgagtc cgcccgagac cgccaccaca
     1021 atgcgctcgc cgcgctcgaa gagccggcgc gtcaggatgg ccgtccggac ttcagcaagc
     1081 aggtcagtca tggcaagaac attagaccgc tccgagcatt gcagcgcaat ggcccgaggt
     1141 ccgcgcggca agccgcggac ctcagaggga cgatctcgag agaatcctcg atcccgtcaa
     1201 cgcagcacac agacctgacg cgtctgtaac gagcttcacc ggtcaagcat cccaagaaca
     1261 cgcgagaccc gcgtcacgag tagacgagga acaggacaag attgagcaaa aggacggcga
     1321 tcaccacacc ccagaggagg cgtttggcgg actttgcatc gtctggctgg ctcatcaggc
     1381 gctgaccgcg tctaggcttc ttcgacgacc ttcagttcga gggtggcagc caactccatc
     1441 accgcgttgc ggaaatcgcc atagcaggtg acccgatgcc aaccccattg gtcccaatac
     1501 cggaacaatt tctcgatgtc gccaatcggt tcggcgcaga gcttggtccg gcaggcccgg
     1561 tcgtccaaat cgttcgccac cgtccgcgcg cgatgcatga cgacctcctt ccgcttctgg
     1621 tcgaactgaa ccgtggttgt gaggtaacca accggcagaa tggaccggac ggatgcgccc
     1681 tgacgatcct cggaatgcgt catgatatcg aacggactcg ctttgccgcg cgggccgaac
     1741 ggcttgttcg acgccacgca atgggcatag acgatttcgc gcttggacgt gtcgatcacc
     1801 ggatcggaaa tgtagccggg ccggccatgg ctcaggatgg tcagaatgat catggccgac
     1861 gtggaccgca cgtcggcttc gcacgcgcca accatgcctt cgttgagcaa ttgatggaaa
     1921 ccgaggcacg gataggcatg gatatgcccg ccgtagaaac cgcccaggca atcgatcgtg
     1981 atcgcgttgg cgatgtactt cttgagcagc gccttctggg ccagatacat cgccgcactc
     2041 tcttcaagcg tcttgcgcgt cacatc
//
LOCUS       DAOQRO010000431         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_69200_length_2050_cov_4.271178, whole genome shotgun sequence.
ACCESSION   DAOQRO010000431 DAOQRO010000000
VERSION     DAOQRO010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69200_length_2050_cov_4.271178"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1184)
                     /locus_tag="PKK20_12680"
     CDS             complement(57..1184)
                     /locus_tag="PKK20_12680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV00786.1"
                     /translation="MKITPYGAAGGDVTGSAYLVETSEAKILVDCGLFQGGKEAEAKN
                     QAPVVPRAQDLDAVLLTHGHLDHTGRLPLLTMAGFKGPIHATPATIEMTGLILRDSAK
                     VQAQDLERHNRKRARAGEPSLAPLYTHDDVERLLEHLRPAPYHDPFPVAPGIRACMVE
                     AGHMLGSVSLQLLFEDEGRERAVVFSGDLGPLGAPILRDYEPFCRADAVVLESTYGNR
                     DHRPFRETVEQFVGVLQQAVADRGKVLVPTFAVGRAQVLTMLLASVFRKGLVKPFPVF
                     LDSPMAIEASRIYQRHLELFDDEMLDFLREGSIERDLATLQLTATADESKKINDVSGP
                     CVVMAGAGMCNAGRILHHLKQNLWRPETHVVIVGYQRFCFR"
     gene            1360..>2050
                     /locus_tag="PKK20_12685"
     CDS             1360..>2050
                     /locus_tag="PKK20_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HNV00787.1"
                     /translation="MIVPIPNELATVLAILPELEQAYLVGGCVRDALLGVPVKDYDIE
                     VFGVSCEQLAAALERHGRVDLVGHSFGVLKFTMHGGRTFDFSLPRHDSKIRSGHKGFR
                     IVTNPAMTLADAAARRDFTINALMFDPRRNEVLDFFNGRRDLDERVLRHTSPAFAEDP
                     LRVLRGMQLAARFDLQPAPETVALCRTIKGACGELAAERVREEWFKWASQSVRPSAGL
                     RVLEATEWLEHF"
BASE COUNT          363 a          646 c          663 g          378 t
ORIGIN      
        1 tcagatgatg cagaatgcgt ccggcgttgc atgtgcaacg ccggacgcat tctgcatcat
       61 ctgaaacaga acctctggta tccgacgatc accacgtggg tttccggcct ccagaggttc
      121 tgtttcagat gatgcagaat gcgtccggcg ttgcacatgc ccgcgcctgc catcacgaca
      181 cagggtcccg acacgtcgtt gatcttcttg gattcatccg cggtggctgt caactggagc
      241 gtggccagat cgcgctcgat cgatccctcg cgcaggaaat cgagcatctc gtcgtcgaac
      301 agctccagat gacgctggta aatcctcgat gcctcgatgg ccatcggact gtcgagaaag
      361 accgggaacg gcttgaccag ccccttccga aacaccgagg ccagtagcat ggtcaggacc
      421 tgggcgcgtc cgacggcaaa ggtgggcacc agcaccttgc cgcggtccgc cacggcttgc
      481 tggagaacac cgacgaattg ctcgaccgtc tcgcggaagg gacggtgatc gcggttgccg
      541 tacgtggact cgagcacgac tgcgtccgct cgacagaacg gttcgtagtc ccggaggatg
      601 ggggcgccca ggggccccag atcgcccgag aacaccaccg cgcgctcgcg cccttcgtcc
      661 tcgaacaaca gttgaaggct caccgagccg agcatgtggc ccgcctcgac catgcaggcc
      721 cgaatgcccg gcgcgaccgg gaacggatcg tgataaggtg cggggcgcag gtgctcgagc
      781 agacgctcga catcgtcgtg ggtgtagagc ggtgccagcg acggttctcc cgcccgagcc
      841 cgcttgcggt tgtgccgttc gagatcctgg gcctggacct tggcggaatc gcgcaggatc
      901 aaccccgtca tttcaatcgt cgccggcgtg gcgtgaatcg gtcccttgaa gccggccatc
      961 gtgagcagcg gcaggcgtcc ggtgtgatcc agatgcccgt gcgtcagcag caccgcatca
     1021 aggtcctgtg cccggggcac gaccggcgcc tggttcttcg cctccgcctc cttaccgccc
     1081 tgaaaaagcc cgcaatcgac caggatcttg gcttcagaag tctcgaccag gtaagcggac
     1141 cccgtgacat cgccgcccgc ggcaccgtag ggagtgatct tcatcgttcc ggccgggacc
     1201 atacttggat gcgaccgggt tcgcaaagag gatttggccg gcgagagcgc cccggtgcat
     1261 ccggttgttg tcggtgtttg tatcgcccgg ctagggcgcc gagttgcagc ggccgcgctg
     1321 gctgaaaccg gggatcgcgg gtagagttgc ggcgattcga tgatagtgcc gattccgaat
     1381 gaactggcga ccgtgcttgc gatcctgccc gagttggagc aggcctatct cgtcggcggc
     1441 tgcgtacgcg atgcgcttct cggcgttccc gtcaaggatt acgacatcga ggtgttcggc
     1501 gtgagctgcg aacaactggc cgccgccctc gaacgccatg gacgcgtgga ccttgtcgga
     1561 cactcgttcg gggtcctcaa attcacgatg cacggcgggc gcactttcga cttcagcctg
     1621 ccccgacacg actcgaaaat tcggtccggt cacaagggat tccgaatcgt tacgaatccc
     1681 gcgatgacgc tcgcggatgc cgcggcgcgc cgcgatttca ccatcaatgc gttgatgttc
     1741 gatccgcgcc gcaacgaggt gctcgatttc ttcaacggcc ggcgggatct cgacgagcgc
     1801 gtgttgcgac acacgagccc ggcgtttgcc gaggatccgc tgcgcgtgct gcgcggcatg
     1861 caattggccg cgcgcttcga tttgcagccg gcgcccgaga cggtggcgct ctgccgcaca
     1921 atcaagggag cctgcgggga actggccgcc gagcgggtgc gcgaggagtg gttcaaatgg
     1981 gcatctcaga gcgttcggcc ctcagccgga ctccgagtgc tggaagcgac ggaatggctc
     2041 gagcactttc
//
LOCUS       DAOQRO010000432         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_85187_length_1803_cov_4.753432, whole genome shotgun sequence.
ACCESSION   DAOQRO010000432 DAOQRO010000000
VERSION     DAOQRO010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85187_length_1803_cov_4.753432"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PKK20_12690"
     CDS             <1..1605
                     /locus_tag="PKK20_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00788.1"
                     /translation="YQGVLVGEPLAAPFARPGMGAWGFQNGHAASANGIRAPDGVALH
                     EPPSSSEPPMLVGITNLSLQFRAADATRPLQQVDLFVDGVLAFTVTNLPPAEGNAVSI
                     GIGPTSFDWTVPAGATLESVATNLATLVNSQSNATLVAATAVGDRIRLISLLPGRSGE
                     DVSLTAIESQGSGPSLTTLVHPAQPRFVDSAARPWRSYTIDATAAEGDVLQCTLIKTN
                     GESITVGATNPPAGTTANLVRQVLQEVNASPMLQGPDGVSAEDIFVMSDKLVSFFLYA
                     RSPGLAAAQIECAWSAPDHFQMDPSAPVRLDENRPDLAPRNHLYVTAGSAKLDLTFPF
                     DSAAYPDGHHELTAVAYEGSSVRTQTRVTTRVRICNTPLEATLRCPVGQTEFAVEAVM
                     PFDVMANRTDIDRIELFGTGGWLAAMTNAQTAAFVLPGTELGAGQHSVVAVVTASDGA
                     RYQTAPVEFRLIGPEVPVTVAIFPAPPTLSWTSVYGRTYEVLEGATATHIGEVAATLT
                     ASTTGITSWRIPVPLGTARFYRVRAR"
BASE COUNT          317 a          629 c          543 g          314 t
ORIGIN      
        1 tatcagggtg tcctcgtggg tgagccgctg gccgccccct tcgcccgtcc cggtatggga
       61 gcatggggct ttcaaaacgg ccacgccgcc tcggccaacg gaatccgcgc accagacggc
      121 gtcgcgctcc atgaaccgcc gtcatcgtcc gaaccaccga tgctcgtggg aatcactaat
      181 ctcagcctgc aattccgggc cgcggatgcg acgcgtccct tgcagcaggt ggatttgttc
      241 gtggacggtg tcctggcctt caccgtgacc aacctgcccc cggcggaagg caacgccgtc
      301 tcgatcggga tcggtccgac cagcttcgac tggaccgtgc ccgcgggagc gactttggag
      361 tccgtggcaa ccaacctcgc cacgttggtc aacagccagt ccaatgccac cctcgtcgcc
      421 gccaccgcgg tgggcgatcg catcaggctg atctcgctcc tacccggacg ctccggagag
      481 gatgtcagcc tcaccgccat cgagtcgcag gggagcggac catccctgac aaccctcgtc
      541 caccctgccc aacctcgatt cgtcgattcc gcggcgcggc catggcgctc ctacacaatc
      601 gacgcgaccg cggccgaagg cgatgtcctg caatgcacac tgatcaagac taacggcgag
      661 agcatcacag tcggagccac caatccaccc gccggcacca cggccaatct ggtccgccag
      721 gttctgcaag aagtgaacgc atctcccatg ctgcaggggc cggacggggt gtcagccgag
      781 gacatcttcg tcatgagcga caagcttgtc tccttcttcc tctatgcccg ctcgccgggc
      841 ctcgccgcgg cccagatcga atgcgcgtgg tccgctcccg atcacttcca aatggacccc
      901 tcggcgccgg tgcgacttga tgagaaccga cccgatctgg cccctcggaa tcacctctat
      961 gtgaccgccg gaagcgcaaa gctcgatctg actttcccct tcgacagtgc agcctacccc
     1021 gacggtcacc atgaactgac agccgtggcc tatgaaggca gttcggtgcg gactcaaacc
     1081 cgcgtcacca ctcgagtccg catctgcaac accccgctcg aagcaacctt gcggtgtccg
     1141 gtcgggcaga ccgagttcgc ggtcgaagcc gtgatgccat tcgatgtgat ggcgaatcgg
     1201 acggacatcg atcggatcga actgttcggg actgggggct ggctggccgc aatgacgaat
     1261 gcgcagacgg cggcattcgt cttgccggga accgaactcg gcgcgggtca acacagcgtg
     1321 gttgccgtgg tgaccgcctc cgacggcgcc cgctaccaaa ccgcgcctgt tgaattccga
     1381 ctgatcggac ctgaggttcc ggtcaccgta gccatttttc cagcgccgcc gacgctttcc
     1441 tggacctcgg tgtacggccg cacgtacgaa gtgctcgagg gcgcgacagc gacgcacatc
     1501 ggcgaagtgg cagcaacgct caccgcatcg accaccggca tcacatcctg gagaatcccg
     1561 gtgccgcttg gaaccgcgcg gttctatcga gtccgtgccc ggtaaccggc gcggcgtccc
     1621 ggcccatgtt ccgacgtccg tgtcgggctg gtccgcgcca gtgcatctga agattggcgg
     1681 tgttctgtcg cccggtgagg gcgccgggcc tccaacggat ggagactcac gctgtaggcc
     1741 gcgtgccttg tctgcgccgc aaagcgcagc ggcctgtctg ccgtgtcggg cacggcacag
     1801 gca
//
LOCUS       DAOQRO010000433         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_90974_length_1732_cov_4.806202, whole genome shotgun sequence.
ACCESSION   DAOQRO010000433 DAOQRO010000000
VERSION     DAOQRO010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90974_length_1732_cov_4.806202"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1732
                     /locus_tag="PKK20_12695"
     CDS             <1..>1732
                     /locus_tag="PKK20_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV00789.1"
                     /translation="TGFGSHLWADIVGKDVLRVKTSASYEVFYGNNGGNDIDGALLFL
                     SGFSAFNDVHPGPQFRPEPVPEGLVGRGVTTVESGNWIALTPLTLPALRPGQTGKAIF
                     GFVAGEVSNDSFVEVATLIPTAGACCANLTAAGGGVHANQAVASGVAPAPMRRGGAQL
                     AGVFPGIPDQDRAALNEALRVAWVYYPYDFAPMRWKQGHACYEAALHLQEQLYYTADA
                     PGNPMVNWRITVITKNLAGGHTSVLLTSPDGSRVYMVDNFLYPGVIPMVKVGADKWQI
                     DPRWYLANPSTIIGVLDTIFMGGYLNPWVPLGGLQVKCAVPYTGLGKKTIRVVGSADP
                     NDKSGLTGSGSARWIAGSETLAYTIDFENVPTATAPAQQVVVTDALDPRIDWTTVELG
                     DIRFNNLDMTINPGLQHFETTTRVATDPHPVRVSADFSPATGLLSWRVVSVDPVTGSA
                     PEDPFAGFLPPNKANRVGQGSVRFSARLRPGLAHGTTITNAASIVFDVNAPILTPLYV
                     NTIDALPPASAVHALPAEAWSEFILTWSGQDEGSGVAGYDIYVARDGGPYELWLSQTS
                     DTSARFSGQLG"
BASE COUNT          304 a          571 c          531 g          326 t
ORIGIN      
        1 gacgggtttc ggctcgcacc tctgggccga cattgtcggc aaggatgtcc tccgggtgaa
       61 aaccagcgca agctacgagg tgttctacgg caacaacgga ggcaacgaca tcgacggcgc
      121 cttgttgttc ctgtctggct tctcagcgtt caatgatgtg catcccggcc cccagttccg
      181 gcccgagccg gtccccgaag gtctcgtcgg acgtggcgta acgaccgtcg aatccggcaa
      241 ttggattgcc ttgaccccat tgacgttgcc ggcgctgcgc ccgggccaga ccggcaaagc
      301 gatttttggt ttcgtggcgg gcgaagtgtc gaacgactcg tttgtcgagg tcgccacgtt
      361 gattcctact gcaggcgcgt gttgcgccaa cctgactgcg gccggtggag gagtgcatgc
      421 gaaccaggcc gtagcgtccg gcgtcgcgcc ggcaccgatg aggcgcggag gcgcgcagtt
      481 ggccggcgtg tttccgggga ttcccgacca ggatcgggcc gccctgaacg aggccctgcg
      541 ggtggcctgg gtctattacc cgtacgattt cgcgccgatg cggtggaagc aggggcatgc
      601 gtgctatgaa gccgcgctcc atctgcagga gcaactctac tacaccgctg acgcccctgg
      661 gaatccaatg gtcaactggc ggattaccgt catcaccaag aatctcgccg gcggtcacac
      721 cagcgtcctc ctcacctcac ccgacgggag ccgcgtatac atggtggaca actttctcta
      781 tccgggcgtg atcccgatgg tcaaggtggg agcggacaaa tggcagattg acccgcgctg
      841 gtacctggca aatccctcga cgatcatcgg cgtcctcgat accatcttca tgggcggcta
      901 tctgaatccc tgggttccgc tgggcgggct tcaggtcaag tgcgccgtcc cttacaccgg
      961 tctcggcaag aaaacgatcc gggtggttgg ttcggccgat ccgaacgaca aaagcggtct
     1021 gaccggctcc gggtcggcgc gctggattgc cgggagcgag accctcgcct acaccatcga
     1081 ctttgaaaac gtcccaacag ccaccgcccc ggcgcaacag gttgtcgtca ccgatgcgct
     1141 cgatccccgg atcgactgga ccacggtcga actgggggac attcgattca acaacctgga
     1201 catgacgatc aatcccggct tgcagcattt cgagaccact accagggtcg cgaccgatcc
     1261 ccatcccgtg cgggtgagtg ccgatttcag cccggcaacc ggcctgctct cctggcgtgt
     1321 cgtctctgtc gaccccgtga ccggcagtgc gccggaagac cctttcgccg gattcctccc
     1381 gccgaacaag gcgaatcgag ttggccaggg atcagtccgg ttcagcgccc ggctccgccc
     1441 cgggctcgcc catggcacga cgatcaccaa cgctgcgtcc attgtcttcg atgtcaacgc
     1501 gccgatcctc acgcctctct acgtgaacac catcgacgcc ctgcctcccg caagtgctgt
     1561 ccatgccctt ccggctgagg cctggagcga gttcatcctc acctggagtg gacaggatga
     1621 aggctccggc gtggcgggct acgacatcta cgtggcccgc gacggcggcc cctatgagct
     1681 gtggctgagc cagacctcgg acaccagtgc gcggttctcc gggcaactcg gg
//
LOCUS       DAOQRO010000434         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_98561_length_1651_cov_5.754386, whole genome shotgun sequence.
ACCESSION   DAOQRO010000434 DAOQRO010000000
VERSION     DAOQRO010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98561_length_1651_cov_5.754386"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="PKK20_12700"
     CDS             complement(<1..1590)
                     /locus_tag="PKK20_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV00790.1"
                     /translation="MKATLEDEQRKALDALLASTNAVSVFRGGAGTGKSYVLRELVEQ
                     LRESGRCVVVLAPQRQQVVDMEKAGLPSPSTVANFLLKGELATGAVVVVDEAGQIGGR
                     QMLELLRFAREQNARVILSGDTRQHGAVEASDALLAIERHSGVKPVELHRIRRQDPAL
                     GRDDEERDRIRAYRKAVESAAAGKVGESFGRLDKMGAVVACGLADQAERLADEYLQLT
                     ERDVSAVVVSQTWAEVHRVNSRVRDALKEKGLLGVNDTVVQALDRLDLTTAQKRDERF
                     YPEDAVVVFNQKIRQAEPGAKGKLAGIVKAGVLVEVGGRFVTVSNKMLDRISVCLPRE
                     VAVAAGDRLHLKANRKLASGGRATNGELVTVKSVGTDGGIELTDGRVLDREFREFLAG
                     YAVTSYGSQGKTVDYVLFSDSTIKAATSAQQWYVTISRGRRGIRIFTPDKEQLRENVT
                     RSSHRPLALDLAPGIAPRRGVRLWDRLHGHLLRFGRRAADTFRLLRLSRRRHQSIEIH
                     EHKITRMLGERPERSRGQNRSI"
BASE COUNT          306 a          553 c          464 g          328 t
ORIGIN      
        1 gatacttcga ttttgaccgc gcgagcgttc gggtcgctcg cccaacattc gggtgatttt
       61 gtgctcatgg atttcaatgg attgatgacg acgacgagaa agtctcagca ggcgaaaggt
      121 gtcggcggcc cgtcgaccga accgcagcag atgcccgtgc agccgatccc ataggcgaac
      181 accgcggcga ggcgcgattc caggcgcgag gtccaacgcc aaaggcctgt ggctagatcg
      241 ggtcacgttc tcacgcaact gttccttgtc aggggtgaag atacggatgc cgcgtcgccc
      301 ccgcgagatg gtcacatacc attgctgcgc acttgtggcg gccttgatgg tcgagtccga
      361 gaacaacacg tagtccaccg tcttgccttg cgacccgtat gatgtaacgg cgtagccggc
      421 caggaattct cggaattccc tgtccagcac ccgaccatcc gtgagttcaa tgccgccatc
      481 ggtgccgact gatttcaccg tcaccagctc gccattggtg gcccgcccgc cggatgccag
      541 cttgcggttg gctttcaggt gcagccgatc tccagcagca acggccactt cgcgtggcag
      601 gcagaccgag attcggtcga gcatcttatt tgaaacagtg acaaaacgtc cgccaacctc
      661 gaccaacacc cccgccttga cgatgccagc caacttgcct ttcgcgcccg gttcggcctg
      721 tctgatcttc tggttgaaga ccacgaccgc atcttccgga tagaaccgtt cgtcacgctt
      781 ctgcgcggtc gtgaggtcca gcctatccag cgcctgaacc acggtatcat tcacccccag
      841 cagtcccttt tctttcagcg catcgcgaac gcgcgaattg actcggtgga cctccgccca
      901 ggtctgcgag accaccacgg ccgacacgtc tcgctcggtg agttggaggt attcgtcggc
      961 aagcctttcg gcttgatccg ccaggccgca ggccaccacc gcgcccatct tatcgaggcg
     1021 accgaatgac tcgcccacct tgcccgccgc cgccgattcg actgctttcc gatatgcccg
     1081 aatccggtca cgctcctcgt cgtcccgccc caacgccgga tcttgccggc gaatcctgtg
     1141 cagttcgacg ggtttaacgc cggaatggcg ctcgatggcg agcaaagcgt ccgatgcctc
     1201 gacagcccca tgctgtcggg tgtcaccgga gaggattacc cgcgcgtttt gttcgcgagc
     1261 gaaacgcagg agttcgagca tctgccgtcc accaatctgc cccgcctcat ccacgactac
     1321 gaccgcccct gtggcgagtt cgcccttgag gaggaaattc gcgactgttg acggtgacgg
     1381 cagtcccgcc ttttccatgt ccacgacctg ctgacgctgc ggggcgagga caacgacgca
     1441 ccggccagac tctcggagtt gttcgaccaa ctcacgcagg acgtagcttt tgcccgtgcc
     1501 cgcgccgcca cgaaacacgg aaaccgcatt cgttgaggcc aagagagcat cgagcgcctt
     1561 gcgttgctca tcctccaatg tcgccttcac tggtcgcgga ttggccacca gcggatggca
     1621 ggcggcaacg ccatctttcg cggtctggac a
//
LOCUS       DAOQRO010000435         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_102030_length_1617_cov_5.001921, whole genome shotgun
            sequence.
ACCESSION   DAOQRO010000435 DAOQRO010000000
VERSION     DAOQRO010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102030_length_1617_cov_5.001921"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..>1617)
                     /locus_tag="PKK20_12705"
     CDS             complement(237..>1617)
                     /locus_tag="PKK20_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNV00791.1"
                     /translation="GGALDRIHHDLFAARNDPKAGRLCGLGFVNEGLDFNPVVYDFLF
                     ELAWRDQPVDLTAWLREFARRSYGRANADAEMAWEILHQTVYQSAFDNPVAYTAVPSL
                     GGPPGTPYNNDRLANAGRLLLDAADALGGSDAFRFDLVNVTRQALGNDSAEFHQRIID
                     AYERKDRDELRSAMDDFREFLQDMDALLGTRPEFLLGGWLDDARRWGRTEPERRRFEW
                     NARRVITLWGDTTALRDYASRQWSGMLTGFYLERWELFAKHLDQELASNQPFNSDAFH
                     KEMFAFEKQWAESPDRSPPNARGDSISLARRLWAKYGALSTPESPSLTTGKPVACSSS
                     LDPHPAFLANDGRARSTEAFWATDVGRHPGDAWWQVDFEQPTTIARVIVVGYYGDDRF
                     YGFKIETSMDGAEWTLAADCRENRARSTRSGYTCRFEPRQARYLRITQTNNSANTGRH
                     LVEVMAFAE"
BASE COUNT          287 a          458 c          575 g          297 t
ORIGIN      
        1 ccggccatag gctcgggctg aggtcgtaga aggtgttgag cggcggcgtg gcggggaggt
       61 tggcgcccaa ggcggtgaag gcggttcgca gggaatcgct gcgaaggacg cgatacgtgc
      121 ggttggttga gtcccagcag ggtcagccct taagaaacaa gtttcgccgt ggttgtaaaa
      181 gatggcacgg agaaactgaa ttcttaaggg ctgaccctgc tcggttgttg cggctttcac
      241 tcggcgaagg ccatgacctc gaccaggtgg cgtccggtgt tggcagagtt gttggtttgg
      301 gtgatgcgga gatagcgggc ctggcgcggc tcgaaccggc aggtgtagcc gcttcgggtc
      361 gagcgggcgc ggttttcccg gcagtcggcg gccagggtcc actcggcgcc atccatcgag
      421 gtctcgatct tgaatccgta gaatcggtcg tcgccgtagt agccgacgac gatcacgcgc
      481 gcgatcgtgg tcggctgctc gaagtccact tgccaccatg catcgccggg gtgtcgcccg
      541 acgtccgttg cccagaacgc ctccgtcgag cgggcgcgtc cgtcgttggc caggaacgcc
      601 ggatgcggat cgagcgagga cgaacaggcg accggtttgc cggtcgtgag gctgggcgat
      661 tccggtgtcg agagtgcgcc atacttggcc cagagtcgcc gcgccaggct gatgctatcg
      721 ccgcgggcat tgggcggaga gcgatctgga gactcggccc actgtttttc gaacgcgaac
      781 atttctttgt gaaaggcgtc tgaattgaac ggctgattcg acgcgagctc ctgatcgagg
      841 tgtttggcga acaattccca acgttcgagg tagaagccgg tgagcatgcc ggaccactgg
      901 cgggatgcgt aatcgcgcaa agcggtcgtg tcgccccaga gggtgatcac gcggcgcgca
      961 ttccattcga accgccggcg ttcgggctcg gtccggcccc agcgcctggc gtcatcgagc
     1021 cagccgccga gcaggaattc cggacgtgtt ccgagcaacg cgtccatgtc ctgaaggaac
     1081 tcccgaaagt cgtccatcgc ggagcggagt tcatcccgat ccttgcgttc ataggcgtcg
     1141 atgatccgtt gatggaactc ggccgagtcg ttgccgaggg cctgtcgagt gacgttcaca
     1201 aggtcgaatc ggaacgcatc cgagccgccc aatgcgtcgg cggcgtcgag cagaagccgg
     1261 cccgcgttgg ccagacggtc gttgttgtag ggcgtgcccg gcggaccacc cagggacggg
     1321 acggcggtgt aggccaccgg attgtcgaag gcggattggt agaccgtctg gtggagaatc
     1381 tcccaagcca tctcggcgtc ggcgttcgcg cgcccgtaag aacggcgcgc gaactcccgc
     1441 agccaggcgg tgagatcgac cggttgatcg cgccaggcaa gttcgaacag gaaatcgtag
     1501 acgacggggt tgaaatcgag gccttcattg acgaagccca gcccgcagag ccgaccggct
     1561 ttcggatcgt ttcgcgcggc aaagaggtcg tgatgaatgc ggtcgagggc gccgccg
//
LOCUS       DAOQRO010000436         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_105667_length_1584_cov_4.776324, whole genome shotgun
            sequence.
ACCESSION   DAOQRO010000436 DAOQRO010000000
VERSION     DAOQRO010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105667_length_1584_cov_4.776324"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKK20_12710"
     CDS             <1..841
                     /locus_tag="PKK20_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HNV00792.1"
                     /translation="TGALSSLERRGKKRIESPLRPDFWRAPTDNDRGRDMVKSQGVWR
                     GAHEEAVVSAFAVTTQPDGRAVVVKTASSLPRVDATWENCYTVLPDGEILVEATFTPA
                     KTDLPKLPRFGTQMILPSGFDRIMWLGPGPHETYWDRNDARVGRYGGTMREQFCYDYV
                     KPGESGNKVNVRWVALTNARGEGLLAIAHPEQALSVNASHHTTEDLQSVTHPCDLPRR
                     DTVILNLDAKQQGVGGDDSWGAWPHPEYLIPCAPQTHRFRLRPIAAGEHPELLARTAW
                     PRL"
     gene            868..1440
                     /locus_tag="PKK20_12715"
     CDS             868..1440
                     /locus_tag="PKK20_12715"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNV00793.1"
                     /translation="MVLVIDNYDSFTYNLVQYLGEMQAELAVHRNDQVTLDQIRALRP
                     ERILISPGPCSPRESGLSNDIIRTFAGQVPIFGVCLGLQCIGHVFGANVVVNHRMMHG
                     KTSLIHHNGRDLFADMPNPFPATRYHSLVIQRESLPACLEVTAQTDEGEIMGVRHREL
                     PVHGVQFHPESILTDGGRTILANFLRLEVS"
BASE COUNT          299 a          528 c          482 g          275 t
ORIGIN      
        1 gaccggcgcg ctctcgtcac tcgaacgccg cggcaagaaa cgcatcgaat ccccgctgcg
       61 acccgatttc tggcgggcgc ccaccgacaa cgatcgaggg cgcgacatgg tcaagtcgca
      121 gggcgtgtgg cgcggcgccc acgaagaggc tgtcgtctcg gcctttgctg tcacgaccca
      181 acccgacggc cgcgcggtcg tcgtgaagac cgcgtcgagc ctgccccgag tcgacgcgac
      241 ttgggagaat tgctacaccg tgctgcccga cggtgagatt ctggtcgagg cgacattcac
      301 cccggccaaa accgacctgc ccaaactccc ccggttcggg acccagatga ttctgccgtc
      361 cggatttgac cggatcatgt ggcttggacc cggaccgcac gagacctatt gggatcggaa
      421 cgacgcgcgt gtgggccgct acggcggcac gatgcgcgag cagttctgtt acgactacgt
      481 caagccgggc gagagcggca acaaggtcaa tgtgcgctgg gtcgccttga cgaacgcccg
      541 aggcgagggg ctgctcgcca tcgcgcaccc ggagcaagcg ctcagtgtga acgcgtcaca
      601 ccacacaaca gaagacctcc agtcggtaac gcatccctgc gatctcccgc gccgcgacac
      661 cgtgatactg aacctcgacg cgaagcagca gggcgtcggc ggcgacgaca gttggggcgc
      721 atggccgcat cccgagtacc tgatcccgtg cgcgccccaa acccaccgct tccgcctgcg
      781 cccgatcgca gcgggcgaac atccggagtt gctggcccgc acggcctggc cgcggcttta
      841 gcctcgtggc actctacgcg ctgtcttatg gtcctggtca tcgacaacta cgattcattc
      901 acctacaacc tcgtccagta cctgggggag atgcaggccg agcttgcagt ccatcgcaac
      961 gatcaggtca cgctggatca aatccgcgcg ctgcggcccg agcgcatcct gatctcgccg
     1021 ggcccttgct cccctcgcga atcgggcctg tcgaatgaca tcattcgaac cttcgcgggg
     1081 caggtgccga tcttcggcgt ttgcctcggg cttcagtgca tcggccacgt gtttggggcc
     1141 aatgtcgtgg tgaaccatcg catgatgcac ggcaagacat cgctgattca ccataacgga
     1201 cgcgaccttt tcgccgacat gcccaatcca ttcccggcga cgcggtatca ttcgctcgtc
     1261 attcaacggg agagcctgcc ggcgtgcctc gaagtcacgg ctcaaaccga cgagggcgag
     1321 atcatgggcg tgcgccatcg ggaactgccc gttcacggcg tccagttcca ccccgagagc
     1381 atcctcaccg atggagggag aaccatcctg gccaacttcc tccgcctcga ggtttcctga
     1441 aaatccggat gtggcgccat caaacccagc gtgttatatc gaggcatacg cgggaactgc
     1501 tgggtaacag tgcgtgagcc gaacgcgggg ctttgatgag gctcgggatg tccacgatca
     1561 cggatttggg tgcgccgaac acgt
//
LOCUS       DAOQRO010000437         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1190
            NODE_108434_length_1560_cov_3.625914, whole genome shotgun
            sequence.
ACCESSION   DAOQRO010000437 DAOQRO010000000
VERSION     DAOQRO010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852533
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:38:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,544
            CDSs (total)                      :: 2,523
            Genes (coding)                    :: 2,489
            CDSs (with protein)               :: 2,489
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 7 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 3 of 34
            Pseudo Genes (multiple problems)  :: 7 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108434_length_1560_cov_3.625914"
                     /isolate="AMR_MDS_1190"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..>1560)
                     /locus_tag="PKK20_12720"
     CDS             complement(196..>1560)
                     /locus_tag="PKK20_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV00794.1"
                     /translation="PWPAAANGGGPSLQLIDPRQDNARVANWAAVTGPATNTQRNLVP
                     IDASWRYWQQSTDPAPGWSNIDYNDLTWPAGRALLYVESARLPAPKNTPLTIGQMSYF
                     FRHSFTFDGNPQGARLRLRPVIDDGAVFYLNGEPVFWLGMAEGKIPERDTPSTRTVVD
                     AAYEGPFEIPVTNLRLGANVLAVEVHQVNATSTDIVMGVSVDVIEMPREQYTPAYANS
                     TRATLAPFPDLWINEVLPLNETGLLDNAGDRDPWIELANFGLEPAAMGDWFLSNTYAD
                     PARWPFPNNASIAAGQFKLVWADAEPAETTAVDWHANFRLVGPSGVVVLSRLQQGQPA
                     VVDFLEYTELAADQSFGYPAPRLEDAGPVVLPEPTPGAANLLEPPAPPEFLALEIDSA
                     GAVTLRWTTVPGWTYRVEASHDVPFGAWDALGEIVATGAEASFTDTSAVERNTRFYRL
                     RIEW"
BASE COUNT          268 a          419 c          596 g          277 t
ORIGIN      
        1 aacccccgac caagggctta tgagtcccct gctctaccgc tgagctactc tggcacaacc
       61 ggcaaggcca gcattatgct gcggtccaat gggctgacaa gcagttttgt caggggtgtc
      121 ggcgccggtt tcgtgatgcc cctcgaggct tgagaatctg gctcgagaat ctgatttgtc
      181 cgaaaagacg cctgcttacc actcgatgcg caaccgatag aagcgggtgt tacgttcgac
      241 ggcggaggtg tcggtgaagc tggcctcggc gccggtggcg acgatttcgc ccagggcatc
      301 ccacgcgccg aaggggacgt cgtgagaggc ttcgacgcga taggtccacc ccgggacagt
      361 ggtccagcgg agcgtgacgg cgccggcgct gtcgatctcg agtgcgagga actcgggcgg
      421 cgcgggcggt tcgaggaggt tggcggcgcc gggcgttggc tcgggcagca cgacaggtcc
      481 tgcatcctcg agacgggggg caggatagcc gaaggactgg tcggcggcga gttctgtgta
      541 ttcgaggaaa tcgacgacgg cgggctggcc ctgctggagg cgcgacagga cgacgacgcc
      601 tgaagggccg acgaggcgga agttggcgtg ccaatcgaca gcggtggtct cggcgggctc
      661 ggcgtcagcc cagaccaact tgaattggcc ggcggcgatg ctggcgttgt tggggaaggg
      721 ccagcgggca ggatcggcat aggtgttcga gaggaaccag tcgcccatgg ccgccggttc
      781 gagaccgaag ttggccaact cgatccaggg atcgcggtcg ccggcgttgt cgagcaggcc
      841 ggtctcgttg agcgggagca cttcattgat ccaaagatcg gggaaggggg cgagcgtggc
      901 gcgggtcgag ttggcgtagg cgggcgtgta ttgctcgcgc ggcatttcga tcacgtcaac
      961 actgacgccc atgacgatgt cggtgctggt ggcattgacc tgatggactt cgaccgcgag
     1021 gacgttggcg ccgaggcgga ggttggtcac tggaatctcg aacgggcctt cgtaggccgc
     1081 gtcaaccacg gtgcgggtcg agggtgtatc gcgctcgggg attttgcctt cggccatgcc
     1141 gagccagaag acgggttcac cgttgagata gaacacggcg ccgtcatcga tgacgggccg
     1201 gagccggagg cgcgcgcctt gcggattgcc gtcgaaggtg aaggagtgtc ggaagaagta
     1261 gctcatctgg ccgatggtca ggggtgtgtt tttgggcgct gggagacggg ctgactcgac
     1321 gtagagcagc gcgcggcccg cgggccaagt caagtcattg taatcgatgt ttgaccagcc
     1381 gggcgcggga tcggtggact gctgccaata gcgccaggaa gcatcgatgg gcaccaggtt
     1441 gcgttgggtg ttggtggcgg gtccagtgac ggccgcccag ttggcgacgc gcgcgttgtc
     1501 ctgacggggg tcgatcaact gaagcgaagg ccctccgccg ttggctgccg cgggccaggg
//
